LOCUS       DAMJYC010000001        98869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJYC010000001 DAMJYC010000000
VERSION     DAMJYC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 98869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.001"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="PC472_000001"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="PC472_000001"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            286..477
                     /locus_tag="PC472_000002"
     CDS             286..477
                     /locus_tag="PC472_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2725380.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            632..2041
                     /locus_tag="PC472_000003"
     CDS             632..2041
                     /locus_tag="PC472_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2725381.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2172..2861
                     /locus_tag="PC472_000004"
     CDS             2172..2861
                     /locus_tag="PC472_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2725382.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2864..3799
                     /locus_tag="PC472_000005"
     CDS             2864..3799
                     /locus_tag="PC472_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2725383.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3796..4689
                     /locus_tag="PC472_000006"
     CDS             3796..4689
                     /locus_tag="PC472_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2725384.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4767..5513
                     /locus_tag="PC472_000007"
     CDS             4767..5513
                     /locus_tag="PC472_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2725385.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLIQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5514..6347
                     /gene="rsmI"
                     /locus_tag="PC472_000008"
     CDS             5514..6347
                     /gene="rsmI"
                     /locus_tag="PC472_000008"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2725386.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6370..7074
                     /locus_tag="PC472_000009"
     CDS             6370..7074
                     /locus_tag="PC472_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2725387.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDRDFFPIHLRKHVSNYLKTYIEEGINKLNVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7262..7690)
                     /locus_tag="PC472_000010"
     CDS             complement(7262..7690)
                     /locus_tag="PC472_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2725388.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7727..9157)
                     /locus_tag="PC472_000011"
     CDS             complement(7727..9157)
                     /locus_tag="PC472_000011"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2725389.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9289..9873
                     /locus_tag="PC472_000012"
     CDS             9289..9873
                     /locus_tag="PC472_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2725390.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMKRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9889..10410
                     /locus_tag="PC472_000013"
     CDS             9889..10410
                     /locus_tag="PC472_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HDF2725391.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10476..10722
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10806..12743
                     /gene="metG"
                     /locus_tag="PC472_000014"
     CDS             10806..12743
                     /gene="metG"
                     /locus_tag="PC472_000014"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2725392.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKGELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12773..13543
                     /locus_tag="PC472_000015"
     CDS             12773..13543
                     /locus_tag="PC472_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2725393.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13881..14312
                     /gene="phnG"
                     /locus_tag="PC472_000016"
     CDS             13881..14312
                     /gene="phnG"
                     /locus_tag="PC472_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2725394.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14327..14902
                     /gene="phnH"
                     /locus_tag="PC472_000017"
     CDS             14327..14902
                     /gene="phnH"
                     /locus_tag="PC472_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2725395.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14905..15990
                     /locus_tag="PC472_000018"
     CDS             14905..15990
                     /locus_tag="PC472_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2725396.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFISELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16010..16879
                     /locus_tag="PC472_000019"
     CDS             16010..16879
                     /locus_tag="PC472_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2725397.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16872..17738
                     /locus_tag="PC472_000020"
     CDS             16872..17738
                     /locus_tag="PC472_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2725398.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17779..18492
                     /locus_tag="PC472_000021"
     CDS             17779..18492
                     /locus_tag="PC472_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2725399.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18493..19662
                     /locus_tag="PC472_000022"
     CDS             18493..19662
                     /locus_tag="PC472_000022"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2725400.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19666..20502
                     /locus_tag="PC472_000023"
     CDS             19666..20502
                     /locus_tag="PC472_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2725401.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20503..21498
                     /locus_tag="PC472_000024"
     CDS             20503..21498
                     /locus_tag="PC472_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2725402.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21516..23204
                     /locus_tag="PC472_000025"
     CDS             21516..23204
                     /locus_tag="PC472_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2725403.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23216..24283
                     /locus_tag="PC472_000026"
     CDS             23216..24283
                     /locus_tag="PC472_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2725404.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24720..25715
                     /locus_tag="PC472_000027"
     CDS             24720..25715
                     /locus_tag="PC472_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2725405.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25733..27421
                     /locus_tag="PC472_000028"
     CDS             25733..27421
                     /locus_tag="PC472_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2725406.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27433..28500
                     /locus_tag="PC472_000029"
     CDS             27433..28500
                     /locus_tag="PC472_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2725407.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28692..29222
                     /gene="rnmV"
                     /locus_tag="PC472_000030"
     CDS             28692..29222
                     /gene="rnmV"
                     /locus_tag="PC472_000030"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2725408.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29393..30262
                     /gene="rsmA"
                     /locus_tag="PC472_000031"
     CDS             29393..30262
                     /gene="rsmA"
                     /locus_tag="PC472_000031"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2725409.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEIGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30328..31590
                     /locus_tag="PC472_000032"
     CDS             30328..31590
                     /locus_tag="PC472_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725410.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDSEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31741..32856
                     /locus_tag="PC472_000033"
     CDS             31741..32856
                     /locus_tag="PC472_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2725411.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKVLGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            33042..33938
                     /locus_tag="PC472_000034"
     CDS             33042..33938
                     /locus_tag="PC472_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2725412.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            34008..34805
                     /locus_tag="PC472_000035"
     CDS             34008..34805
                     /locus_tag="PC472_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725413.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34860..36212)
                     /locus_tag="PC472_000036"
     CDS             complement(34860..36212)
                     /locus_tag="PC472_000036"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2725414.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36431..37309
                     /gene="purR"
                     /locus_tag="PC472_000037"
     CDS             36431..37309
                     /gene="purR"
                     /locus_tag="PC472_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2725415.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37465..37743
                     /gene="spoVG"
                     /locus_tag="PC472_000038"
     CDS             37465..37743
                     /gene="spoVG"
                     /locus_tag="PC472_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2725416.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37956..39335
                     /gene="glmU"
                     /locus_tag="PC472_000039"
     CDS             37956..39335
                     /gene="glmU"
                     /locus_tag="PC472_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2725417.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39430..40380
                     /locus_tag="PC472_000040"
     CDS             39430..40380
                     /locus_tag="PC472_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2725418.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41220..41305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41467..41988
                     /locus_tag="PC472_000041"
     CDS             41467..41988
                     /locus_tag="PC472_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2725419.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            42140..43816
                     /locus_tag="PC472_000042"
     CDS             42140..43816
                     /locus_tag="PC472_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2725420.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43828..45036
                     /locus_tag="PC472_000043"
     CDS             43828..45036
                     /locus_tag="PC472_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2725421.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            45051..46763
                     /locus_tag="PC472_000044"
     CDS             45051..46763
                     /locus_tag="PC472_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2725422.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46756..47664
                     /gene="pilO"
                     /locus_tag="PC472_000045"
     CDS             46756..47664
                     /gene="pilO"
                     /locus_tag="PC472_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2725423.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47691..48257
                     /locus_tag="PC472_000046"
     CDS             47691..48257
                     /locus_tag="PC472_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2725424.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48294..48821
                     /locus_tag="PC472_000047"
     CDS             48294..48821
                     /locus_tag="PC472_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2725425.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48842..50380
                     /locus_tag="PC472_000048"
     CDS             48842..50380
                     /locus_tag="PC472_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725426.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50480..51562
                     /locus_tag="PC472_000049"
     CDS             50480..51562
                     /locus_tag="PC472_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2725427.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51543..52325
                     /locus_tag="PC472_000050"
     CDS             51543..52325
                     /locus_tag="PC472_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2725428.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            52427..53077
                     /locus_tag="PC472_000051"
     CDS             52427..53077
                     /locus_tag="PC472_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2725429.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            53091..53651
                     /locus_tag="PC472_000052"
     CDS             53091..53651
                     /locus_tag="PC472_000052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2725430.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53668..57054
                     /gene="mfd"
                     /locus_tag="PC472_000053"
     CDS             53668..57054
                     /gene="mfd"
                     /locus_tag="PC472_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2725431.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            57085..58080
                     /locus_tag="PC472_000054"
     CDS             57085..58080
                     /locus_tag="PC472_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2725432.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFDDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58204..58743
                     /gene="spoVT"
                     /locus_tag="PC472_000055"
     CDS             58204..58743
                     /gene="spoVT"
                     /locus_tag="PC472_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2725433.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58863..60476
                     /locus_tag="PC472_000056"
     CDS             58863..60476
                     /locus_tag="PC472_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2725434.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60583..62031
                     /gene="mazG"
                     /locus_tag="PC472_000057"
     CDS             60583..62031
                     /gene="mazG"
                     /locus_tag="PC472_000057"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2725435.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            62120..62398
                     /locus_tag="PC472_000058"
     CDS             62120..62398
                     /locus_tag="PC472_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2725436.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62470..62712
                     /locus_tag="PC472_000059"
     CDS             62470..62712
                     /locus_tag="PC472_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2725437.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            62774..63028
                     /locus_tag="PC472_000060"
     CDS             62774..63028
                     /locus_tag="PC472_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2725438.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            63029..63562
                     /locus_tag="PC472_000061"
     CDS             63029..63562
                     /locus_tag="PC472_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725439.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63568..63852
                     /locus_tag="PC472_000062"
     CDS             63568..63852
                     /locus_tag="PC472_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2725440.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63965..64885
                     /locus_tag="PC472_000063"
     CDS             63965..64885
                     /locus_tag="PC472_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2725441.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64975..67347
                     /gene="spoIIE"
                     /locus_tag="PC472_000064"
     CDS             64975..67347
                     /gene="spoIIE"
                     /locus_tag="PC472_000064"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2725442.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGSLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67698..69590
                     /gene="pepF"
                     /locus_tag="PC472_000065"
     CDS             67698..69590
                     /gene="pepF"
                     /locus_tag="PC472_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2725443.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69704..70669
                     /gene="galU"
                     /locus_tag="PC472_000066"
     CDS             69704..70669
                     /gene="galU"
                     /locus_tag="PC472_000066"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2725444.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            71169..72773
                     /gene="rho"
                     /locus_tag="PC472_000067"
     CDS             71169..72773
                     /gene="rho"
                     /locus_tag="PC472_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2725445.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72926..73126
                     /gene="rpmE"
                     /locus_tag="PC472_000068"
     CDS             72926..73126
                     /gene="rpmE"
                     /locus_tag="PC472_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2725446.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73271..74164
                     /locus_tag="PC472_000069"
     CDS             73271..74164
                     /locus_tag="PC472_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2725447.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74182..75030
                     /gene="prmC"
                     /locus_tag="PC472_000070"
     CDS             74182..75030
                     /gene="prmC"
                     /locus_tag="PC472_000070"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2725448.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            75074..76138
                     /gene="prfA"
                     /locus_tag="PC472_000071"
     CDS             75074..76138
                     /gene="prfA"
                     /locus_tag="PC472_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2725449.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76267..76947
                     /locus_tag="PC472_000072"
     CDS             76267..76947
                     /locus_tag="PC472_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2725450.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            76976..78016
                     /locus_tag="PC472_000073"
     CDS             76976..78016
                     /locus_tag="PC472_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2725451.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            78033..78482
                     /locus_tag="PC472_000074"
     CDS             78033..78482
                     /locus_tag="PC472_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2725452.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78499..78951
                     /gene="rpiB"
                     /locus_tag="PC472_000075"
     CDS             78499..78951
                     /gene="rpiB"
                     /locus_tag="PC472_000075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2725453.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            78987..79616
                     /gene="upp"
                     /locus_tag="PC472_000076"
     CDS             78987..79616
                     /gene="upp"
                     /locus_tag="PC472_000076"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2725454.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79911..80726
                     /locus_tag="PC472_000077"
     CDS             79911..80726
                     /locus_tag="PC472_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2725455.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80952..81389
                     /locus_tag="PC472_000078"
     CDS             80952..81389
                     /locus_tag="PC472_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2725456.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81574..81801
                     /locus_tag="PC472_000079"
     CDS             81574..81801
                     /locus_tag="PC472_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2725457.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81806..82192
                     /locus_tag="PC472_000080"
     CDS             81806..82192
                     /locus_tag="PC472_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2725458.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82195..82899
                     /gene="atpB"
                     /locus_tag="PC472_000081"
     CDS             82195..82899
                     /gene="atpB"
                     /locus_tag="PC472_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2725459.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            82968..83228
                     /gene="atpE"
                     /locus_tag="PC472_000082"
     CDS             82968..83228
                     /gene="atpE"
                     /locus_tag="PC472_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2725460.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83351..83848
                     /gene="atpF"
                     /locus_tag="PC472_000083"
     CDS             83351..83848
                     /gene="atpF"
                     /locus_tag="PC472_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2725461.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83845..84390
                     /locus_tag="PC472_000084"
     CDS             83845..84390
                     /locus_tag="PC472_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2725462.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84407..85909
                     /locus_tag="PC472_000085"
     CDS             84407..85909
                     /locus_tag="PC472_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2725463.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85937..86815
                     /gene="atpG"
                     /locus_tag="PC472_000086"
     CDS             85937..86815
                     /gene="atpG"
                     /locus_tag="PC472_000086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2725464.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86828..88222
                     /gene="atpD"
                     /locus_tag="PC472_000087"
     CDS             86828..88222
                     /gene="atpD"
                     /locus_tag="PC472_000087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2725465.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88225..88485
                     /gene="atpC"
                     /locus_tag="PC472_000088"
     CDS             88225..88485
                     /gene="atpC"
                     /locus_tag="PC472_000088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2725466.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88947..89327
                     /locus_tag="PC472_000089"
     CDS             88947..89327
                     /locus_tag="PC472_000089"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2725467.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89342..89803
                     /locus_tag="PC472_000090"
     CDS             89342..89803
                     /locus_tag="PC472_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2725468.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89824..90414
                     /locus_tag="PC472_000091"
     CDS             89824..90414
                     /locus_tag="PC472_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725469.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90429..91586
                     /gene="alr"
                     /locus_tag="PC472_000092"
     CDS             90429..91586
                     /gene="alr"
                     /locus_tag="PC472_000092"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2725470.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91619..91906
                     /locus_tag="PC472_000093"
     CDS             91619..91906
                     /locus_tag="PC472_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2725471.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91875..92258
                     /locus_tag="PC472_000094"
     CDS             91875..92258
                     /locus_tag="PC472_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2725472.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92429..93256
                     /locus_tag="PC472_000095"
     CDS             92429..93256
                     /locus_tag="PC472_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2725473.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93249..94190
                     /locus_tag="PC472_000096"
     CDS             93249..94190
                     /locus_tag="PC472_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2725474.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94613..95707
                     /locus_tag="PC472_000097"
     CDS             94613..95707
                     /locus_tag="PC472_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725475.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95933..96349
                     /locus_tag="PC472_000098"
     CDS             95933..96349
                     /locus_tag="PC472_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725476.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96531..97100)
                     /locus_tag="PC472_000099"
     CDS             complement(96531..97100)
                     /locus_tag="PC472_000099"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2725477.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLNKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            97254..98411
                     /locus_tag="PC472_000100"
     CDS             97254..98411
                     /locus_tag="PC472_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2725478.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        39586 a        10675 c        18140 g        30468 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatt ttttttattt
      181 tttgtcaaaa aaaacaaaaa atacgtattt cttttttcat ttcgggaaaa ctaaacacag
      241 gaattatgga ctttataaaa atttaattct tgaggagtga tataagtgaa aaaagttgct
      301 ttaattttag ctataatagg tgcgcttaac tggggtgcaa ttggtattct aggtacagac
      361 ttaataggta gtatctttgg tggaacatat gaaatggtaa gtagaataat atacttcatt
      421 gtaggtttag ctggattata cttaatacca agtttaatgt ctgatgatag agaataattt
      481 atgcttttat gttaaatacc tcattctaaa tgtttagaat gaggtattta attttataaa
      541 aatattaatt tgtttctttt ttaatggtaa aatagataag aattgaagtt ttaaaaagaa
      601 gtattatgta aaaaaggagt actattaata tatgtttatt attaatgaat taaataagat
      661 tgtaaatgaa gaagtaatat cttttcatat gcctggtcat aaaaagggac aggtttataa
      721 aaagttagga tataaagata tactaaaaaa tttgtataaa atggatacaa ctgagatatt
      781 aggaacagat aatttacact ttccggaagg aattataaaa aattcacaaa aaaaagcatc
      841 aagagtattt aaaagtgatt atacttgcta tttagtaaat ggtagtagct gtggaataca
      901 atctgcaata atgtcagtat gtagtcctaa aagtaaaata ataacaaata gagactgtca
      961 tcagtctgtt ataaatggat gtattttagg agatatagat atagagtatg tgccttgtga
     1021 gataagtgaa gagacaaata tcttaaaagg gatatctata agtaatgtga ttgatataat
     1081 agataaaaac ttggatgcaa aagcagtact gcttacttat cctacatatt atggaatgac
     1141 atatgattta gaatacatat gtaattatgc acatagcaaa aagatggtag tgatagtaga
     1201 tgaagcacat ggtgcgcatt tagggcttag cgagaggctt cctaaaacag ctcttgagca
     1261 aggcgcagat atagttgtac agagtacaca taaaactctt ccagcattta cacaatcgtc
     1321 aatgatacat atacagggag accgagttga tatagataaa atattgaaca tgttaagaat
     1381 tacagaatct tcaagccctt catatttatt attatcttct ctggaattag cagtagatat
     1441 atatgaaaat aaaggaaaag aattaatgga agagctttta aataatatta taactttcaa
     1501 aaataaagtt gattcaaata ttaatatata cgacacaaat gataaaacaa aaatatttat
     1561 atcatctaaa aacatgggtt taacaggtta tgaactagaa aatattttga gaaataaata
     1621 taacatacaa gtggagttat ctaattatta tggagttctt ttaatatgta caataggaaa
     1681 tactgttgag gatttctcta gtttagaaac agcattaaac aatattgtaa aggaagactt
     1741 taaatttgaa aagttagaaa ataaaaaata ccctgttgtt attccaaaga aaatattaac
     1801 acctagagaa gcattttata aaactaaaaa aagtgttaaa atatatgaca gcataggtaa
     1861 agtttgtggt gaatgtatag ttccataccc acctggaata agtgtaatat ctcctggtga
     1921 agtaataagt aaagaaataa tagactatct aatgttttgt cattctaaag gcatgattgt
     1981 gagtggattg aaagatgtag atttaaattt tatacaaata atagatttag aagacattta
     2041 aaaattatga tataaaagta gatagaagta gatagtttaa aattgtgaag agaaataaat
     2101 agactgctta tatagagcta tctaataaaa gtactaaaaa agtaaaaagt gtttgtatgg
     2161 aggatatgaa aatgaaaggt aagttaataa ttatagagag tggttcagat gcaagtggaa
     2221 aggctactca gacagaaata ttatatgaaa gattaaaaaa agaaggctat atgattaaaa
     2281 aggtagaata tccaaattat gaatctccat catctgcctt ggtgaaaatg tacctaaaag
     2341 gagactttgg taaaaatgca tcagatgtag acccatatat agcatcaact ttttttacag
     2401 ctgataggta tgcatcattt aaaaccgatt gggaggagtt ttacaatgaa ggtggaataa
     2461 ttatcgcaga tagatataca acctctaata tggtacatca ggcctctaaa atggatattt
     2521 gtgatagaga agagtattta aattggttag ctgattatga atttaattta tttaagatac
     2581 cacaacctga ttgtgttata tttttagatg tgcctattga atttagtaaa aagcttatgg
     2641 agaatagaaa aaataaaatt acaggggaag aaaaaaagga tattcatgaa agtgacatag
     2701 agtatttaga gaaatcttac aacaatgctt tatatatagc agataaatat gattggaaaa
     2761 aaataaattg tgttttagat aacaaattaa gaagtattga aagtatacat aatgaaattt
     2821 ataaaatagt tcttgattca ataaaaccta tggagagata aatatgtatt ttgaaaatat
     2881 tatagggcaa gattttgcaa agaaatattt aacaaattct ataaagaaaa ataaacttaa
     2941 taatgcgtat atgtttgaag gaatggatgg aataggaaaa aagaaatttg cagatgaatt
     3001 atcaaaacta ttacttgatt atgaaaatct cgaaaatagt ccagactatg tactcataaa
     3061 accagatgga aatagtataa aaatagctca aatcagaaat ttacaatcag atatagtaat
     3121 aagacctcat aaagactata aaatatatat aatcaataat gctgaaaaaa tgactgtgga
     3181 agctcaaaat gcacttttaa agacgctaga ggaacctcca aactatgcta taataatctt
     3241 agtcacaaac aacaaggaat ctttattaga aactataaaa tctagatgtg atataataaa
     3301 gttctcacct ataccaattg aagacttaaa acgttatttg ataaatacag gtatagaaga
     3361 agaaagagct caattattgg ctattttttc gagaggaagt attgagaatg ctctaaatct
     3421 atctcagtct tctgagtttt caatgatgag agaagatata caacagtata ttcagataat
     3481 gttagataaa aacatagtgg agattttaaa tataccaaat aatatggaaa aatatagagg
     3541 taagattata gctttattag atatgatgat aaactatttt cgagatatca tcctattaaa
     3601 agaaaatgta aacaagaaca tgttaataaa tgtagataag ctagttttca ttcaaaatat
     3661 gagtggaaaa attagttatt ctcaattgtc caagattatt gatataatag aagatgcaaa
     3721 gagtaaaata aaaagcaatt gcaatttcaa tataagcata caagttatgt ctttaaatat
     3781 atacgaggtg attaaatgat aaagatagta ggtataaggt ttaaaagtgc tgggaaaata
     3841 tattattttg accctgttga ttttaatata gagcaagata tggatgtagt tgtagaaact
     3901 gcaagaggct tggagtatgg gaaagtagtc gttggtccaa aagatatgga tgaaagcgaa
     3961 ttagtttctc ctttgaagcc aattataaga atagctacag aagaagataa aaaagtatat
     4021 ctagaaaata aggaaaaggc aaaagaaact tttgagttgt gccaacaaaa aataaaagaa
     4081 catgagttaa caatgttttt aatagattgt gagtatacat ttgataggaa taagttgatt
     4141 ttttacttta cagcagaagg aagaatagat tttagagaat tggtaaaaga cttagctgct
     4201 atatttaaaa ctagaataga acttagacaa ataggtgtca gagatgaagc taaatctata
     4261 ggaggtctag ggccttgtgg tagaaaactt tgttgttcat cttggttagg tgattttcaa
     4321 ccagtttcaa taaaaatggc aaaagaccaa agtctatctc taaatccaac aaaaatatct
     4381 ggaatatgtg ggagattatt ctgttgctta aaatatgagc atgatgtata tagtgaagca
     4441 atagaaaaaa tgcctgttgt aggtgcattg gtgcaatcag atgatggaaa aggaaaagtt
     4501 gtagaagtga atcctttatt agaacaaatt aaagtggaat ttcaagacaa gacagtaaag
     4561 atatacacaa gagaagatat aaaaatactt agagagccta aaaaatgtga tggctgtggt
     4621 ggtaaatgta aggataatga tggattagat gaagccacac taagagagct aaagaaatta
     4681 gaagattaaa tttaaggtag ggcttttcct acctttttta aagcaattaa tgcttaaagt
     4741 tattaatatt tgaggagaat ttatatatgc aattaaaaga ttcagaaaga atagatgatt
     4801 tacaattaaa aggccttaaa attatacaag atactaatgg tttttgtttt ggtatagatg
     4861 cagttttatt agccaatttt actaaaataa aaaaagatgc caaagtagta gatttgggta
     4921 ctggaactgg cattatacct attttgatag ctggaaaaag tgaagctaaa aaaattattg
     4981 gcgtggaaat acaagaagat gtatatgaaa tggcaactcg ttctataaaa ttaaatggat
     5041 tagaagaaag agttgagatt ataaatgaag atataaaata catagacaaa gtcttagaca
     5101 taaatggtta ccatgtagtc acatcaaatc ctccatatat gcatattgat ggaataaaaa
     5161 atccaaatga taaaaaagca atatcaagac atgaagtaag gtgcaatttg gaagatgtaa
     5221 ttagagctgc atccagactg gttatgccta gaggaaaatt cttcatgata catagaccaa
     5281 caagattggt tgatataata actttaggga gaaaatataa attagaacca aaggttattc
     5341 aatttgtgca tccaagacct caaaaagcac cgaacttagt tttgattcaa tttgtaaaag
     5401 atggaaggcc agagcttaaa atattggacc cattgtatgt ttatggagaa gatgggaatt
     5461 atacaaaaga attaaaagca atatataata ataaagatat aggagagttg taaatgagcg
     5521 ggaaattgta catatgtcca acaccaatag gaaacctaga agatataaca tatagaactt
     5581 taagaatatt aaaagaagta gacttgattg cagcagaaga tacaagacat agtataaaac
     5641 ttttaaatca ttttgaaatc tcaaaaccac taactagtta tcatgaacat aataaggatt
     5701 caaagggaga ttatttgata aataaactaa ttgatggtga aaatatagct ctgataagtg
     5761 atgcaggtat gcctggtata tctgacccag gagaagagat aataaaacaa gctatacaaa
     5821 ataacataga gatagaagta ttgccaggtg caacagcctt tgtaacggct ttagttggct
     5881 ctggtatgga cacacataga tttgtttttg agggcttttt agatagagat aaaaaagtta
     5941 ggaaagcaca attggaagaa ttaaaggaag aaagtagaac tataattttc tatgaatcac
     6001 cacatagatt gaaggataca ctaaaagata tgcttaaaat tcttggtaat aggaatattt
     6061 ctataaatag agaacttaca aaaaaatatc aagaaataat aagagaaaat atagaaacta
     6121 ccataaaaat atttgaggaa aaagaagtaa gaggagagtt tgtattaata gtagagggtt
     6181 ttcatggtga gaagtcaaag aaagaaaatt atgatgggct tacagatagg gaatatgttt
     6241 taaaactaat tgaaagtggt atgaacaaaa aagatgcaat aaaaatagtt tgtaaagata
     6301 gaaagttaaa gaaagatgtg gtttataaac aagttttaga tttataaaac ctgaatctga
     6361 ggtgatttta tggaaatagg aaaaattata atagagagtt taaagaggga agatacacat
     6421 aaaatattta tagaattaga taataagcat gaattagaaa aaataattcc taaaataagc
     6481 tgtatgaagt ctgtaggtga atgtaaatat catgtagtaa attgttttga acattctata
     6541 aatgcattaa aagaattaga aatagtatta aatgataggg atttttttcc aatacatcta
     6601 agaaaacatg tgtcaaacta tttaaaaaca tatattgaag aaggaataaa taagttaaat
     6661 gtattaaagc tgggaacatt tttacatgat ataggtaagc ctgacagtat gaccttagat
     6721 gaaaccggaa gagttcattt cacaaatcat gaaaaaatag gtgctcaaat aattgacaat
     6781 atgggaataa agttagagtt atcaacagag acatatagac ttataagtaa atatgtaaga
     6841 tatcatatga tacttttgtc tctatataag aaaaatgatt tgagtaggaa agaattaatt
     6901 aatgtattta acttagtaga tgaggatact ataggtgtaa taatgcttgg gtatgcagat
     6961 atagtgtcta cagtaaaatt attgggcaag agtggagaag tcagtgtgtt aaagacatat
     7021 atggaatata ttttaacaaa ttacttgtat aagagtaatt atacacatgt ataaatttag
     7081 ttaatataat tgaattttag atgattaatt gaagtttgaa atatacggat ttcaaaaaca
     7141 taaaagaagt tgtctttgtt gtaaaattat gaaagacaac tttttttata tcaaaaattg
     7201 aaattttatt aaggatactt aatggtctat taattaataa accattagta tttaatttat
     7261 attacaatat tgattttgct tgtattggcg ttgatagtat aacagaggtc ttagtagaac
     7321 caattttctt tatagtatca atcacatttt ctagctcgta catatctttt acaattactt
     7381 ttaaaagtaa gcaatcatca cctgttatat ggtgacattc tacaatccta gggtcctttg
     7441 cagctgactc aataaattct gtatatccat tgcttggaag agaaatatga ataaatgcct
     7501 ttataactct gcctaatgaa tctgggttga caatagcttt atatccttct ataacaccag
     7561 actcttctaa tcttttgact ctttctgaaa ctgcaggaga agttaaacca actatttttc
     7621 ctaagtcctt catagaaatt ctcccatcat cctgtaaaat ttctatgatt ctgtaatctg
     7681 taacatccat aaataaacag tcccccttaa ataaaaataa ttaatgctat aaaacttttt
     7741 tagaatctat catttgtgtt tttaaatacc aaagatttct agataaatca accttataaa
     7801 tgtgtggttt tgaaagtctt ttgtattgtt cccagatagt acttaattca tattgtacat
     7861 gattttttat ttcaagaaca gcttttttat tatatttgca tctgcctttt atatacaatg
     7921 gtttaagaag ctctttaact ttgtaatttg taaatacctt cgttttccat gtataagtag
     7981 gatggaagat ttctaaaggt ttagactcat ctataacttc atcatgtaac ataatcaaat
     8041 ctgcctctgc cttattgtct tcgttatata ttcttataac tttcttgtaa ccaggattat
     8101 ttattttttc tggattctca gaaatcttta tcttaggttc aagaactcct ttttcacaag
     8161 atgcagctaa tttataaact cctccaagag aaggtgaatc gaaagatgta atgagctttg
     8221 tacctactcc ccatgaattt atagtagctc cttctgcttt aagtgatgtt atggtatatt
     8281 cgtctaggtc attagatgca gtgatgctga tatctgaaaa gccagcatta tctaattgtt
     8341 ttttagcttc tttagataaa taagctaaat ctccagagtc aagtcttata cccataggtt
     8401 tgtatccttt ttcagaaata tttttgaata cttttattgc attaggaact ccactgttta
     8461 atacatcata agtatctaca agtagtaggc atttatctgg ataaacatca gcatatgctt
     8521 ggaatgcttc aagttcatta tcaaattttt gtacccaact gtgagcttgt gtaccaatta
     8581 taggtatatc aaacattttc cctgctagta cgtttgcagt tccagcacaa ccaccaacta
     8641 cggcagccct agccccatat atccctgcat ctggaccttg tgcacgacgt aatccaaatt
     8701 caaatacagg gtctccttga gctgcaaagc aaactctaga tgctttagta gcaataagag
     8761 actgaaaatt aactattgtt aacaatgctg tttcaataag ttgagcttga tataaaggtg
     8821 ccttaacagt aattaatggc tcattaggga acatgatagt tccttcctca acagcatata
     8881 tatctcctgt gaatttaaat tcttttaaaa attttaagaa cttgtcagaa aataaattta
     8941 agttttttaa gtactctaaa tcttcatctg aaaaatgaag attatctatg tattcaacaa
     9001 cctgatcaat accacaaact atagtatacc ctccatcaca agcgtttttt ctaaaaaaca
     9061 tatcaaatac aacaatgtct tcatgaatat tcttttcaaa atagccattt aacatagtaa
     9121 gctgatataa atcagtaaga agtgttagat ttctcattat ttctctcctt aattattatt
     9181 aaatattaaa aattgtaaaa actcccacac ttatactaaa acataatata gtacaacttt
     9241 caagtataaa gtattaccta actatcataa aattatttaa agaggaggat gattataata
     9301 ggaaaaatat ggttttatat ggtatcgata ggaataatag gaagcttgat ttctaaaaat
     9361 atgggtgaat taaataaagt tatattaagt gaaacatcaa aaggtataga gtttgcaata
     9421 agtctagctg gtatcatggc actctggatg gggataatga atatcgcaaa agaatcaggt
     9481 ttaattgaaa agataggtca aaaactaaat cctataatga aaagattatt tccttctatt
     9541 ccaaaaggtc ataaagcaat gtcttatata gttatgaata ttgctttaaa tatgttgggt
     9601 gctggaaatg gtgcaacagc atttggttta aaagcaatga gtgagcttca aactttgaat
     9661 aacaagaaag atacagccac taatgatatg attatgttta tggtaatcaa tatatcatct
     9721 atacaaataa ttccatttac tataatcaaa ttaaggatgg atatgggggc tcaaaatcca
     9781 agtgaaataa tttttacgac tttatttgca accataatat ctacagcagt agcaataata
     9841 acatgtaaat tttttcaaag aagatataga taaaggggat gtagatacat ggagatattt
     9901 tcaaacttac ttataccaat aataattctg tacatagtaa tttatggtaa atataaaaaa
     9961 atagatgtgt atgatagttt tgtaaaagga gcaattgatg gattaaaagc tgcatgggat
    10021 atcctaccat atataatagg tatatttttg gcaataggaa tattcaaaac tggtaaaggt
    10081 cttgatatgt tagaatggct atttacacca atagcaaata tgatgagtat accaaaagaa
    10141 ttaataggtc taataagtgt aaaaccttta tctggaagtg gtgctttagg gatgtacagt
    10201 gaacttgcaa atagagttgg aataggaagt ttagtagaaa aaatgggtgc tacgattgta
    10261 ggttcttctg agactatttt ttacacaatg gcaatatact atggaagtct gaagataaaa
    10321 aatactagac atacattatc atgtgctatg attagccatg ttgcaggtgt catagcagct
    10381 gtttttattt gctatgtaat atttgtataa tttgttgaca atatgggaaa aattttataa
    10441 tatagtgatt atatagaaac aaatagtaat acaaattaca agctatgaaa agaagagtaa
    10501 ataaaggttt tttctatcag agagctaggt taggtgaaag ctagtgtcaa aagctttatt
    10561 gaagatggtc ttggagcttt ttatctgagc aagtgtgcta gggtaaaacg gttggacacg
    10621 ttatagtcta ctaaggtatc tataaaatgt ttctacattt catgtgaaac ataactatta
    10681 tagatgaatt agggtggtac cgtgaataat ctcgccccta tgttaactca taggaggcga
    10741 ttttttatta tttaaatgaa agaatcatat gattacgaac gataaaaaat taggaggtaa
    10801 ataacatgag caaaccgagt ttttatgtaa caacaccaat atactatcca agtggaggat
    10861 tacacatagg acatacttat tcaacagtag ctgcagatac aatagctaga ttcaagcgtt
    10921 tctgtggata tgatgtaaag ttcttaacag gaacagatga gcatggtgaa aaaattcaaa
    10981 agaaagccat agaacaagga atgtcagaga tagagtatct tgatggaatg ataaaagata
    11041 taaaggcctt atggaatact atggatatat cttatgatga ttttataaga actacagaaa
    11101 aaagacatac agacataata caaaagatat ttactaaact atatgagcaa ggtgatatat
    11161 ataaaggtga atatgaaggt agatactgta ctccttgtga gagtttctgg acagaatctc
    11221 aattactaga aggaaataaa tgccctgact gtggaagaga aacatattta gtaaaagaag
    11281 aatcttattt ctttagatta tctaaatatg aagacagatt aaaagagtta tttaaagatg
    11341 atagtttctg ttttcctgct gctagaaaaa atgaaatggt ggcaaacttt ttagacaaag
    11401 gattagaaga tttaagcgta actagaacga cttttgactg gggtataaaa gttccttttg
    11461 atgaaaaaca tgtaatttat gtgtgggtag atgctttatg taactatata acagcattag
    11521 gttatatgac tgataatgat gaagaattta aaaaatattg gcctgccaat gttcaaatag
    11581 taggtaaaga aatagtgaga ttccatacaa taatatggcc agcgctttta atggctttag
    11641 gtctagaagt tccaaaacaa gtatttggac atggatggat actatttgct gatgataaaa
    11701 tgagtaaatc aaagggtaat gtggtttatc cagaacctat aatagaaaga tatggaatag
    11761 acacacttaa gtacttctta ttaagagagt ttgcatttgg gcaagatgga agttatactc
    11821 atagaaattt tgtaacaaga ataaattatg atttagcaaa tgatttagga aacttaataa
    11881 gtagaactgt agctatggtt gaaaaataca ataatggtat aataccaaca gcaaaagttt
    11941 caactgattt tgatgctgac ttaaaagaac aagctgtttc aactagagaa aactttgaag
    12001 ctgaaatgga taaaatgcaa ttccatgaag cattagagag tgtatggaaa ttagttagaa
    12061 gaactaacaa atatatagat gaaactatgc cttgggcact agctaaagat gaaacgaaga
    12121 aaggtgaatt agacacagtt ttatataatt tatgtgaatc tataagaata atcgcaacac
    12181 taataaatcc tataatgaat gagactgcta ataaaatata tgaacatata ggaataaaag
    12241 gtcaagatga tataactaca tgggaaagta caaaaacatt tggtcttatt ggagaaaatg
    12301 taaaagtatt taaaggtgaa cctttattcc caagattaga tgtagaaaaa gagatagaag
    12361 aattaactaa aatgttctct gggaaacctc cagtagaaga aaaaccttta gagcataaag
    12421 aagaaattac tattgatgat ttagacaaaa tagagcttag agtagggaaa ataataagct
    12481 gtgaaaaaca tccaaaagca aataaattat tagtatcaca agttaagatt ggaccagaaa
    12541 caagacaaat agtatcagga atagctgaat actataagcc agaggattta gtaggaaaag
    12601 aggttacagt ggtatgtaac ttaaaaccag ttaaattaag aggtgtagaa tctcaaggta
    12661 tgatattagc agcaggcgat gatggagacc catatgtttt accatttaca caaggggcta
    12721 aagatggatg cgaggttcgt tagtaggtaa ttaaatagta aggagattaa agatgttatt
    12781 tgactcacat gcacatttaa atgatgaaag ttttgatgaa gatagagatg aacttattgg
    12841 ttcattaaaa gataaaggtg tagatttggt tgtaaatcca ggagcagata tagagacgtc
    12901 gataacagca attgaacttg cgaaaaaata cgactttata tattcagcag ttggggtaca
    12961 tccacatgat gtatcaaaac tagatgatac agcaatagaa actctaagaa aacttgcaac
    13021 tgagaatgaa aaagttgtag ctattggaga gattggatta gattattatt atgattattc
    13081 tccaagagaa gaacaaaaag agtggtttaa gaagcaaatt gaattagcta atgagctaaa
    13141 acttccaata ataatacatg atagagatgc acatggagat acttttgaga taataaagaa
    13201 tactaaaaat cctgaaatag gatgtgtact tcattgttat agtgggaatg tagagttagc
    13261 tagagaatat gttaagatgg gttgttatat atcaattcca ggcacggtta cttttaagaa
    13321 taataaaaaa actagagaag tagtaagaga aatacctcta gaaagattat tcatagagac
    13381 tgattcacca tatatgtcac cagagccaca tagaggaaag agaaacaatc cttcccaagt
    13441 atcttttgtt gctgataaga tagcccagga aaaagggata tcttatgaag aagtatgtag
    13501 agttacaaaa gaaaatgcaa agaaattttt taatataaaa tagacaaatt aaatcttata
    13561 atgatatata taataagtca ttataagtat aggatgagag tgcctcaaaa tgagtttttt
    13621 agttcatgag gcactctttt ttttatgaaa tcaaatatat tttcatcatt tcaacaatga
    13681 aaaagaagtt tatcatattt tgagatagtt tgtttttatt tatttaattc atattttaaa
    13741 atatattttc ttaacattgt taaaaaagaa tttacaagat attcattaga attaactcaa
    13801 ctctaatatt cacttaactt aagttgttta ttatattatt aaagagaagt aaacgaaagg
    13861 tagattaagg aggttgaatt ttgtatacag ttgatagtac taagatattg ataaatatga
    13921 acttagagaa actagaaaag atatactcaa tgataaaaga tacatcaagt atttctataa
    13981 ttaaagaacc taacttggct acagtaatgg taagagctaa tgaaagtgta aaaaacacaa
    14041 catttaattt aggagagata ttagttactg aatgtagtgt aaaggtagat gaaagcttgg
    14101 gttacggaat agtttcagaa aacaataata agaaagccat ttatttagca gttatagatg
    14161 cagttttaca tagcaataac agtaaatttg atgagttaaa gaattacata aataaaagtg
    14221 tatatgaaga gaacttaagg tatgaacaag agattattga tgaatttagc ttgattaata
    14281 aaacgaaagt tcaattcaat gctatggatt aaataggagg agaatcatgg taaattatac
    14341 acaagaaata tatagaaaga tgctagatag tgcttcattt cctgggaaat taaataatat
    14401 agataatata aatatagaaa ataatacaaa actattgaat ggagcaattg gtatagcaat
    14461 cactttatta gaccaagaag ttaccttcta tatagaaaat tatactcaaa aagatataaa
    14521 aaatataaga gcgcttacaa tgtcaaatca aatagattat agaaaatcag attatatatt
    14581 tttagatatt aattcagaag tggatatatt acagattaaa ataggttctt ttgaatatcc
    14641 tgatgaaagt gcaactatag tacatcaagt aaatgattta tcaacgtact ctcaaactaa
    14701 gtatataaag cttgatttga gtggtcctgg tataaaatct aaaaatagta tatatataaa
    14761 tggaattaga gaagaattta tagaaagatt atctaccata aataaagatt atccaatagg
    14821 aatagatttg attttagtag ataaaaaagg agaaattgca tttataccaa ggtcatcaaa
    14881 attaagttgg gaggtattgt agaaatggct tatgtagcag ttaaaggtgg agaagaagcc
    14941 atattacagg ctaaaaatat acttgagttt tatagggtaa aaggagatag cttgcctata
    15001 gatgtaaagc agattgagga acaaatgtct tatgctgtag acaaagtaat gtctgaagga
    15061 agtttataca acaaggaact atcagcattg gctataaaac aagctcaagg tgatatatta
    15121 gaagcctctt tctatgtaag agctttaagg tcaacaatgc ctagaattac atattcagaa
    15181 cccataaata cagaagaaat gagaataaaa agaagaatat cagctacttt taaagatata
    15241 ccaggaggtc agtatcttgg ggcaactaga gactatgaaa atagaatatt gaatgtagaa
    15301 ttattagaag aagatgaaaa aaagaataga gattttagag atagttttat aagtgaatta
    15361 gaaacagtag atgaaatccc tgaatttaca aaaatatcta atatatttag aaaagaaaat
    15421 ttattacaag aagaagtttg tgaaaacaaa ttggaagaag ttattgatga tattacaaga
    15481 aatagtttaa gatttccagt ttctaggtca gcaaagttac aggcattaac tagaggtgaa
    15541 agtggagtag tgtcaggttt tgcttatgct attgtaagag gttttggtga tgaacatcca
    15601 tatataagtg aacttagaac tggtaatgta tttataaaag taaaacatcc catctatgaa
    15661 gatgaagaaa taataatagg tgatatgctg gttactgaat gtgaaatagt ttcaaaagca
    15721 tcacaaaata agactgaaaa ttctaaaact aattcggaag ataaaaataa gatgtcttta
    15781 ggatatggtc tttgtatggg gaataatgag aataaggcta tatctatggc catactagac
    15841 aaatctttgg agccaaatag tgaaaaacta gcacaagatg aagaatttgt attgcttcat
    15901 atagatggaa tagattcatt aggatttata tctcacttta agcttcctca ctatgtagat
    15961 tttaaagcgg atgtagaaag aataaaatag tttgtggagg taagtttagg tgatatataa
    16021 ctatggtttt ttagacgaaa acacaaaaaa agaaataaga agaaagatat taaaagcaat
    16081 atcaatacct ggttatcaag ttccctttgg ttcaagagag ttgcctatag ctaagggatg
    16141 gggtactgga ggtttacaat taacactatc attaattggt aaaaatgatg tagtaaaagt
    16201 aattgaccaa ggaagtgatg attctacaaa tgcttgtaat ataaggagct ttattgcaag
    16261 tgtaagtaat gtagaaacca caaaagatac attagaagct acattgattc aaacgagaca
    16321 tagaatacca gaagaaaaac ttaaatctaa tcaaatacta atatttcaag tgccaatacc
    16381 agaaaccctt agaatagtag aacctagtga agtagaaaca agaagaatgc attcagaaga
    16441 agattatagt aggatgtggg tatatcttta tgaagatata gttagattta atgatatatc
    16501 tatagcagtt gaatatccat gtaaagtaaa tgatagatat ttaatgaatc catcacctat
    16561 acctagattt gatataaaaa aattaaatat gtcagacaat ttattcttgt ttggtgcagg
    16621 tagagaaaaa agaatttatg ctattcctcc atatacaaag gtagaacctt tagagtttga
    16681 ggattataag tttgaagagg aaaagtttga aggaaaatac tgtagtttat gtaacagtac
    16741 aaatacattt ttagatgaag tatatgacag tgacactaat gaaaagtact atagttgctc
    16801 agatacaagt tattgtgaaa aagttagatt aaaaaataac agtatagatg tgacaatagg
    16861 aggtacttgg aatgagtaaa ataaatgact ctaaaagatt agaaaatata gacaagcaag
    16921 tattaatagg aagaaatatc aatcttattt atggagatgg gtgtgaaaag tgttttgaaa
    16981 gcacaggata tgaaagcaat aatatatgta agtattgtgg ttctatagtt gcatgtaata
    17041 atgtaaattt agaactttat gaaggagaag tccttggaat tgtaggtgaa tctggttcag
    17101 gaaaaagcac tttattaaaa atactgtttt tccaagaaca tgcaaatagt ggagaagtat
    17161 atatatccca ttacaataaa gacacaaata tactagagct ttcagaccaa aaaaagagat
    17221 acataaagaa tcattttatg ggtattgtgt atcaaaatcc ccaccttggt ttaaatttag
    17281 actttagtag tggtggtaat atagcagaaa aattattgat ggcaaattta tataatgttg
    17341 aaaaaataag agatagaggc aaagaacttt taataaaaac taatatacct gtagaaagaa
    17401 tagaccataa accaaaatac tttagtgggg gaatgcaaca acgagttcaa atagcaaaag
    17461 ctctttcaaa taaccctcca atattgctat tagatgaggt tacaacaggt ttagatgttt
    17521 ctgtgcaagc ggaagtattg gatttaataa gagaaatcca aagagaactt aagatttcta
    17581 tgatagttgt atcccatgac tttgatgtta taaagatgct tgctgatagg acaattgtaa
    17641 tgaaaaatgg aagtgtaatt gagtctggtc ttacagacca aataatggag gacccacaac
    17701 acccatatac tcagcaactt ataaattcat tgctataaat atcgtttgta aataagcaaa
    17761 atgggggttt ttaataaaat gaaagaaact atacttgaag taaataattt tagtaaagac
    17821 tttgagttac atgcattgaa caagactata acggcatgta gtaatataag ttttgaagtt
    17881 tcaaaaggtg agtttttagg aataataggt aaatctggag caggtaagtc taccatatta
    17941 aaaagtatct ataaaacata tataccaaca actggagaaa taatttttaa ttcagaaata
    18001 tatggtaatg tagacctggc taaaattgga gaccgagaaa tgataaatct tagaaagaaa
    18061 gaaataggat atgtatcaca gtttttaaag acacttccac gaataactgc aattgaactg
    18121 gtagtacatt ctttaattga gagtgggttt gaaaaagatt gttcgtatga tatggcaaaa
    18181 gatatactta accaatttga aataaaggaa aatttatggg atgcgtatcc aaataatttt
    18241 tctggtggag aaaaattaag attaaattta gctcaagcta tggttaaaaa gcctagactt
    18301 ttgttacttg atgaaccaac tgcatcactt gataatcaat ctaaaatata tgtaaaagaa
    18361 aaaatgttgg agcttaaaag tcaaggaact acaatgatag gaatttttca tgatatagag
    18421 tttatggaaa ctgttataga taagaccttt actatgacaa aagggactat aagtgaaagt
    18481 ggggtagcct agatgataaa actaattcat aatgcaaata ttgtattaga agatatgatt
    18541 ttagaaaatg gtcatttaat catagaggga gatacaataa aaaaagtatc aaatgataag
    18601 attgattacg catttgactt taatgagata gatgaaataa tagatgcaaa aaacttatat
    18661 gtaatacctg gtattataga tattcacagt gatgccattg aaaaggaaat agagccgaga
    18721 ccaagtacat tattaccatt taatatggca ttttatgaac tagataaaaa actacctgca
    18781 aatggaataa ctacagttta tcattctata tctttagggg atggagtagg agttagaagt
    18841 attgataact ctttaaagat gattaaaaat attgattcat ataaaaatat agatagtaag
    18901 agcataaatc ataaagttca tttaagatat gaagtgttgt attatgaagg tttagaaaag
    18961 gttttagaac tattagatga aaataaaata gactacttat caataatgga ccattctcca
    19021 ggacaaggtc agtatacaaa tccaacattt tataaagagt atgctactaa agtgtgggga
    19081 gttacagaaa attatgtaga tacatggtta gatgatttag taaatctgca tgataattta
    19141 gattggaata aaatagctaa tattataggt atagccaaaa ctaaaaatat aaatgttgca
    19201 tcacatgatg atgatacatt agaaaaaatg gatttcataa aaaatatagg cataaatgta
    19261 tctgaatttc ctataactct tgaagttgca aaacattcaa agaaattggg tgttctaact
    19321 tgtttaggtg caccgaatat tgtaagaggt aaatctcata ataacaattt aaaagcaatg
    19381 gatgctatat tggaagattg ttgtgacatt gtgtgttcgg attatcttcc atcagctatg
    19441 ataaagtcta tgtgtatagt tgcagaaaga ataaataatt taaataaagc agtcagttta
    19501 tttacctcaa atcctgctaa agcagttggt atatatgatg aaagaggtag tataaaagaa
    19561 aataaaaaag cagatttagt tttagtagat attgatagtg aatatccaaa ggttgtaaat
    19621 actattgtaa atggaaaaac agtttataaa agggaggtat aaagattgct aacagagtta
    19681 cattgtcata caagtatatc agataataat tttactactg aagaaataat aaaaaaagca
    19741 gcagaaaaga atataaatat cttagcaata acaaatcatg acacattatc tggattggat
    19801 gaagctcttg tgcttggaga gcattatgga ataacaataa taccaggtat agaaatttca
    19861 gcctatgatt ataaaaatag aaaaagagtt catattcttg gatataacat agatttaaac
    19921 agtcaagaaa taaaaaattt gtgcaatcca atggtatgtg acagacataa agcatctgtt
    19981 aagatggtaa ataaaattat agatttagga tacaaaatat ctctggaaga tgtaaagaaa
    20041 tattcatcaa agactggaat atttaaacaa catataatga aagctcttat agataaagga
    20101 tatacagata atatatattc aaatctatat aaagaattgt ttcatagagg aaacggcaag
    20161 gtatatgtat ctttaaaata tgtggattac agagatgcaa taaaagctat aaaaaattct
    20221 ggtggaattt gtgtgttagc tcacccgggt caaatggata attttagcgc tattgaagag
    20281 atggtaaaag taggattaga tggaatagaa gtatatcatc catctcacaa caaagaacta
    20341 gaaaaagagt cgctttctta tgctaagaag tataatctag tggtcacagg aggttcagat
    20401 taccacggat tttattctca acataattat gaacttggaa gcaagagctt aaataaagaa
    20461 gatttattta agtttaggtt agctttagga ggggtattat aaatgtcttt tttacaagta
    20521 aaaaatgtag gtaaaagtta tggacaagtt aaggtgttaa aagacatatc tattgatatt
    20581 gaaaaaggag agtttatttg tttactaggt ccaagtggtt gtggaaaaag tacactactt
    20641 agaataattg cagggcttga ggataaacat ggtggaaaaa taataataaa tgataaggat
    20701 atgactaatt caccacctga aagtagaaat tttggtatag tttttcaatc atatgcatta
    20761 tttccaaata tgaatgttta caaaaacata gcctttggac tagaaaataa aaacataagt
    20821 aaatctaata ttgataaaaa agttaaagaa gttctagaag ttgtagaatt aagtggatat
    20881 gagaaaaaat atccatcaca attatcaggt ggacaacaac aaagggttgc attagctcgt
    20941 gcgattgctt tagagccaga ctttttactt cttgatgaac ctttatctgc attagatgca
    21001 aaagtaagac ttaaacttag ggagcagatt agaagtttac atagaaaact aggcattaca
    21061 actataatgg taacgcatga ccaagaagaa gctctttgtt tagctgataa gatggttgtt
    21121 atgaatagag gagaaataat acaagttgga acgccaaagg aggtatataa aaatccagaa
    21181 acaccttttg tagctgattt tataggaaca ataaatttta tagatgatgg tattaataag
    21241 atagctatta gaccagaaga tataaaagta gagtctaata gagattctaa agataaggat
    21301 ataaaagtgg gagaaatttt agatatcgaa tttagaggat ttaactatag aattacagtt
    21361 gagtatcgtt ctaagcaaat gaaattagat gtagtttcca aagttgcaga acaaatgaaa
    21421 ctgtgcatag gttctaaaat aaactttaag atacctaaag aaggaattgt acaatataaa
    21481 tcagagggat gtgcttaata gctaggggtg aataaatgat aacatcaaaa aataaaagaa
    21541 gaataagtga tagcacaata caaaaatatt tcttgatttt ttcggtaatt gtactaatag
    21601 tatttatatt atttccatta gtatcattat taaaaaatgc atttatggat acaagtggaa
    21661 attttatagg aatttcaaac tttagtaaat atgttgaaaa tccaagtctt atagcatcat
    21721 ttaaaaattc aatttttgta tctacagtct catctttaat atcactatgt ttagcattta
    21781 tatattcata cgcaataaca agaacaaaca taaagtttaa agattttttt aaaactatgg
    21841 gaatgtttcc actttttgca ccaactatgt tatatggtat ttcacttata tacctttttg
    21901 gaaataaggg aatctttaca accatgggtc tggaagtacc actgtatggt ccattaggaa
    21961 taattatttc tcagattcta tttacatttc cacaagcttt tttaatatta tctgtagcac
    22021 tttcgatggc tgattacaga ctttatgaag cagcagatag cttgggtgct agtgaattta
    22081 agaaattttg caaaataaca attcctggta taaagtactc attacttagt tcattctttg
    22141 tatcctttat acttgcattt acagattttg gagcagctaa ggtagtcgga ggaaactaca
    22201 atgtactggc tactgacata tataaacaag tagttggaca atttaacata ccaatgggag
    22261 ctactgtttc tatggtaatg ttaattcctg tactaatagc atttacatta gataagatgg
    22321 caagtaagaa acaaggtatg accataactg caaagtctat gccatataag attaaggaaa
    22381 ataagtttag agatagagta tttttaatag tatgtagttt tataacattg ctaattttta
    22441 ttttaatatt cgtttcagtt ataggttcag ttataaagtt atggccatat aatttaagct
    22501 ttacaatgga acactataca tttaaaggta ttttgggaag tggaataaaa acatacttaa
    22561 gcagtttgaa aatatcaact ttaacagcaa tatttggaac tatatttgta ttttttagtg
    22621 cttatatgat agaaaaggtg gataagtttc ctaaattaag acaaacagct taccttttat
    22681 caatgatacc aatggcctta cctggcttag ttttaggtat atcatatata acttttttta
    22741 actcagcaag taatccatta aattttttat atggaagtac agcaatatta gttatagtaa
    22801 atgtagttca tttttattca gtttgtttta taacctcaaa ttcatcttta aaaatgttgg
    22861 ataaagagta tgaattagtt gcaaaatcca taaatattcc attttataaa gtatttttta
    22921 atataacagt tccaatgtct ataacatcaa tacttgaaat agtagtatat tattttgtaa
    22981 attcaatggt tactgtttca gcattaatat ttttatatac acctcaaact cagactgcat
    23041 caatatcaat actaaagtta gatgagatag gatatatagg accatcagct gctatggcag
    23101 tattagtgct tttaacaaat ataatagtaa gacttttgta cgaatttgta acaaagaaat
    23161 taaaaaataa cacacaaaaa tggcagcaaa aagatgttgc ataggggagg taaaaatgaa
    23221 atttaagaag ctagtttctt taggagtatt atcaacactt ttattaagtg taataacagg
    23281 atgtagtact gattcatcag gttcagattc aaagtctgat agtaagacaa aaggggattt
    23341 aacagtttac acagcaattg aagaagatag tatagagcca tatttagcta catttaaaga
    23401 gaaatatcca gatattaaat taaatattgt tagggcatca acaggagata taacagcaag
    23461 attattagca gaaaaggata atcctcaagc agatgttgta tggggagttg cagccacaag
    23521 cttattggtt gcagatgacc aggggatgtt agaaccatat gctccaaaag gttctgaaga
    23581 aatagagtct gcatttaaag atgataaaga agttccttct tgggttggga tagatgcttg
    23641 gatgacaggt ataacagtaa acactaaaga attaagtgat aagaacatcc cagttcctag
    23701 ttcttacgaa gatttaataa agccagaata taaaggtctt atatctatgc ctaatccatc
    23761 ttcttctggt acagggtact taacagtatc agcccttata caaattatgg gggaagaaaa
    23821 agcatggcag tatatggata agttacatga gaatattggt gtatacactc aatctggttc
    23881 agctccagct acatcagcag cttcaggaga atatcctatt ggtatatctt ttggatatag
    23941 aggtataaag ttaaaagagg aaggctatcc agtagaagta gtatttccaa aagaaggttc
    24001 tggatgggat atagaagcaa atgcacttgt gaaaaaagat aatataaaag aagcatcaaa
    24061 agtattttta gattgggcta taagtaaaga tgcaatgaac gaatactcta agaactatgc
    24121 agttactact atttctacag gaaatccaat accagaagga ttccctaaaa agccattaga
    24181 gcaaatgata gataatgatt taaaatcagc agcaaaaaat agagaagata tactaaataa
    24241 atggatatca aaatatgatg gtaagacaga aaaagagagt taagtaaaac tatacaaatg
    24301 gagtaaaaag aaagaatttt tttaagtaga ttttattata ataatataag ttgagaataa
    24361 tataggtcaa gaaaagtgaa tattctataa aaatgtatag agtaagtata ccattcagtt
    24421 tgaatggtat attttttatt tttagagatt aatttagaaa tagatatact acagtttaaa
    24481 atagtatatt ttaatttcat tattaaagta tagatattat atataaagta aataaattat
    24541 tgagcgatta ttaataagat atgtataaat tttaatttgg gaacttgtgt tatatatcat
    24601 gaaaaaagag aaaaaataaa aggtaaattg tttcttgata acttgaaatt ggaagtgaga
    24661 atatttaact agagaagaat tatttaagtt taagtcagct ttaggaggag gaattataaa
    24721 tgtctttttt acaagtaaaa aatgtaggta aaagttatgg acaagttaag gtgctaaaag
    24781 acatatctat tgatattgaa aaaggggagt ttatttgctt attaggtcca agtggttgtg
    24841 gtaaaagtac attgcttaga ataattgcag gacttgagga taaacatgat ggaaaaataa
    24901 taataaatga taaggatatg actaactcac cacctgaaag tagaaacttt ggtattgttt
    24961 tccaatcata tgcactattt ccaaatatga atgtatataa aaatatagcc tttggattag
    25021 aaaataaaaa tataagtaaa tctgatatag ataagaaggt tagagatatt ttagaagttg
    25081 tagaattaag tggatatgat aaaaaatatc catcacaatt atcaggtgga caacaacaaa
    25141 gggttgcact agctcgtgcg attgctttag agccagactt tttgcttctt gatgaacctt
    25201 tatctgcatt agatgcaaaa gtaagactta aacttaggga acagattaaa agtttacata
    25261 gaaaactagg catcacaact ataatggtaa cacatgacca agaggaagct ctttgtttag
    25321 ctgataaaat ggttgttatg aataatggag aggtaataca gattggaact cctaaagaag
    25381 tctataaaag tccaaaaaca ccttttgtag ctgattttat aggaacaata aattttatag
    25441 atgatgatgt gaatatgata gcaattagac cagaagatat aaaggtagaa tttaatagag
    25501 actttaaaga aattgatgta aaagtaggag agattttgga tatagaattt agaggattca
    25561 actatagaat tacagttgaa tattgttcta agcaaataaa gttggacgta gtttcaaagg
    25621 tagtggaaca aatgaaacta tgtgtaggtt ctaaaataaa cttcaagata cctaaagaaa
    25681 gaaccataca atataaatca gagggatgtg cttaatagct agaggtggat aaatgataac
    25741 aataaaaaat aaaagaagaa taagtgatag taagatacaa aaatgttttt tagttttttt
    25801 agtgattgca ttagtagtat ttatattatt tccattaata tcactattaa aaaatgcatt
    25861 tatggataca agtggaagtt ttgtaggaat ttcaaacttt agtaaatata ttgaaaatcc
    25921 cactcttata gcatcattta aaaactcaat ttttgtatct acaatttcat ccttaatatc
    25981 actatgttta gcgtttatat attcatatgc aataacaaga acgaatataa ggtttaaaaa
    26041 cttttttaaa actatgggca tatttccgct tttcgcacca gctatgctat atggtatttc
    26101 acttatatac ctttttggaa acaagggaat ctttacaatt ataggcatag aagtaccact
    26161 atatggtcca ttaggaataa ttatttgtca aatactgttt acatttccac aggccttttt
    26221 aatattatct gtagcacttt cgatggctga ttatagactt tatgaagcag cagatagctt
    26281 aggggctagt gaatttaaga agttcttcaa aataacaatt cctggtataa agtattcatt
    26341 agttagttcg ttctttgtat cttttatact tgcatttaca gattttggag cagcaaagat
    26401 agttggagga aactacaatg tattggctac tgacatatat aaacaagtgg taggtcaatt
    26461 taacatacca atgggagcta ctgtctctat ggtaatgtta attcctgtac taatagcatt
    26521 tacagtagat aagatagcaa gtaaaaagca agggatgact ataactgcaa agtctatgcc
    26581 atataagatt aaggaaaata agtttagaga tagagtattt ttaatagcat gtagttttat
    26641 aactttatta atctttattt tgatatttgt ttcagttata ggctcagtta taaaattatg
    26701 gccatatgat ttaagcttta caatggaaca ctatacgttt aaaggtatat caggaaatgg
    26761 aatcaaaaca tatttaagca gtttaaaaat atcaatttta acagcaatat ttggaactat
    26821 atttgtattt tttagtgctt atatgataga aaaagtggat aagtttccta gactgagaca
    26881 aatatcttat tttttatcaa tgataccaat ggcattacct ggtttggctt taggtatatc
    26941 gtatataacc tttttcaatt cagcaagtaa tccattaaat tttttatatg gaagtacaac
    27001 aataatggtt atagccaata tagttcattt ttattcagtt tgctttataa cttcaaattc
    27061 atctttaaaa atgttggata gagagtatga attagttgca aaatctataa atattccatt
    27121 ttataaggtg tttttttatg taacacttcc aatgtccata acatcaatac ttgaagtggc
    27181 tgtatattac ttcgtaaatt caatggttac tgtttcagca ttaatatttt tatatatacc
    27241 tcaaactcaa actgcatcaa tatcgatact aaagttagaa gagatagggt atatagggcc
    27301 atcagctgct atggcagtat tagtgttttt aacaaatata atagtaaaac ttttgtatga
    27361 attgacaaca aagaaattaa aaactaagac acaaaaatgg caacaaaaag aagttgcata
    27421 gaggaggtaa aaatgaaatt taggaaatta gtctctttag gaatattatc aacactttta
    27481 ttgagtgtaa taactggatg taataatgct tcatcaggtt caaatttaaa gtctaataat
    27541 aagataaaag gagagttaac agtttataca gcaattgaag aagatagtat agatacatat
    27601 ttaactacat ttaaagagaa atatccagat attaaattga atgtacttag attatcagta
    27661 ggagatataa cagcaagatt attagcagaa aaagataatc ctaaagcaga tgtgttatgg
    27721 ggggttgcag ctacaagttt gataatttta gatgacaaag aaatgctaga gccatacgct
    27781 ccgaaaggct ttgataaggt aaatcctgta tttaaagatg ataagaaagt tccttcttgg
    27841 gttggaatag atgcttggat gactggtata acagtaaata ctaaagaatt aagtgataag
    27901 aacataccag ttcctagttc ttatgaagat ttaataaagc cagaatataa aggtcttata
    27961 tctatgccta atccagcttc ttctggaaca ggatatttaa cagtatcaga acttatacaa
    28021 attatgggtg aagaaaaagc atggcagtat atggataaat tgcataaaaa tattggtgta
    28081 tacactcagt ctggttcagc gccagctata ttagcagctt caggagaata tcccattggt
    28141 atatcttttg gaaatagagg tataaagtta aaaggtgaag gttatccagt agaagtagta
    28201 tttccaaaag aaggttctgg atgggagata gaatcaaatg cacttgtgaa aaaagataat
    28261 ataaaagaag catcaaaagt atttttagat tgggctataa gtaaagatgc aatgaatgag
    28321 tattctaaga attttgcagt tactacaatt gacacgggaa atccggtgcc agaaggattt
    28381 ccaaagaatc cattagaaca gatgatagat aatgatttaa aatcagcagc aagaaataga
    28441 gaggctatac taaataaatg gatagaaaaa tatgatggaa agacagaaaa agagagctaa
    28501 gccttgcaat aaaatttagt acaaaaagcg cctagaatat aagttttata tctaggctct
    28561 tttgatatat ttatttaaaa tgcatatttc tatataaaaa taaatagttt agtaccttga
    28621 ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa acgaataaaa
    28681 ggtgaattta tatgataaaa gaaataatag tagtagaggg tagagatgac gttacagcag
    28741 ttaagagagc aatagatgca gaacttataa ctacaggtgg atttggattt ccaaaaggtg
    28801 taatggagcg tataaaagct gctaatgaaa gacgtggtgt tataatattt acagacccag
    28861 attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga agttccagga tgtaaacatg
    28921 catttctacc gagagaggaa gctaaaaagg atggagatat aggtattgag aatgctactc
    28981 ctcaaagtat aatagcagct ctgaataaag ttagaactga aaatacagaa aaaagagatg
    29041 aatttaagca agttgactta attagaaatg gtcttatagg taatgaagaa gcatcactta
    29101 gaagagatgc acttggaaaa atacttggaa ttggatatgg aaatgcaaaa caatttttaa
    29161 atagattgaa taattatgga gttgaaagag aagaatttat agatgcatta aaacaaatct
    29221 aaatgtaatg tttaatgtat gaaaaatatg tatataaaat aaattgtgga attacggtta
    29281 atttaagata aattatttat aatatatacc ttattttaaa tcaatgtaaa tacatataaa
    29341 taagtataat taataaaatt aaatatcaaa ataagttaaa aaggagtaaa taatggatag
    29401 actatcttca cataatgcaa caaaggaagt agtacaaaaa tataatttta aattttcaaa
    29461 gtctttaggt caaaatttct tgatagatag taatataatt gataaaatct tatctggagc
    29521 tagaatcaca agaggagata atatcataga aataggacct ggtataggaa ctcttactcg
    29581 tgagatgggt aaaatagcag aaaaagtagt ggctatagaa atcgatagaa atttaattcc
    29641 tatattgaaa gataccttgt cagatttaga taatacagag gttgtaaatc aagatatact
    29701 aaaagtagac atacaggaat tagtaaaaga taaattaaat ggtgggccag ttaagcttgt
    29761 agcaaatctt ccatattata taactactcc aatagttatg aaatttttag aggaagacat
    29821 acctgtaaca gacatagtag tcatggttca aaaagaagtt gcagatagaa tgaatgctat
    29881 tccgggtact aaagattatg gggcattatc tatagctgtt caatactatt gtgatacaga
    29941 gattgtggca aaagctccaa gacatatgtt tataccacaa ccaaatgtag attccacagt
    30001 tataggttta catgtaagag ataaaagaaa atatgatgtt cataatgaag acatattttt
    30061 taaaacagta aaagcatcct ttggacaaag aagaaaaact cttcttaatt cgttgggagg
    30121 tcttggtttt cttaataaag atgaaataag agaaatatta aaagaagcta atatagacga
    30181 aaaaagaagg ggtgaaactc ttagtataga agagttttca gttctttcaa atataataaa
    30241 tacaaaagta tcttctaaat agaaggtact ttttttaatt ttgttaacat atattgttta
    30301 gagaaactgt gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa aagagactat
    30361 ataatactag aagctaaaga tataaacttt agatacaaag aacgagtact gccaaaagct
    30421 tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt agaaaatcta
    30481 aaatatgtta aagatgggta taaagcagta gcaataaaag atgaccatgg attaatagac
    30541 ttaggaaata taattttaaa tgaacaagga agaggtgaat ttgttttaga tttagaagat
    30601 tatgatgatg agataaaagg tattgcatta ttgtatgaaa gaaatatacc tttaattgga
    30661 tttaagggaa ataagcttga aaattatgag gacatattat ttcaagaggt agaggaagaa
    30721 gaatatgaag aatatgaaga gttggaagaa gaagacgaag agatagagga taatgaggaa
    30781 agtgatagag acgaagatag tgaagaagaa tatattatag aagaatatga tgaagatgag
    30841 tatgaagact attgggaaga tgatgaagat agtgaagaag gagagttaaa agcaagaacg
    30901 gaagtagaag attctggtga tgaggaaata gaagagttca ataattataa ggaaatagaa
    30961 aattataata gggttaaagt acaggaagaa tatatagaca tagaatatga tgaggaagaa
    31021 gaggaagaag aagtttttga agtaaataga gaagaagata ggtttgtttc ctcaaaacct
    31081 actaaatcaa agaaagttca aaaagatcag aataatattg aaaaaataaa aagagctaat
    31141 ggagtaagtc aaggagagag tggaaataaa caaaccagta cattacttat gccaagacaa
    31201 ataaaaaaag gtttgaaatt atttaaagaa gtaaaacctt ttgtaacaga ttatatagat
    31261 aagacaagat ggtggaaaat agagataaat cctacaacac tgtgtggata tacaatgcct
    31321 tatttaggat atttaaacgt attgaattac actatgtata gtgatgcagt tttacattca
    31381 tataaataca gacactattt atttggagtt cagtatgatg aatataataa gagaaaacat
    31441 tatatttatg ctatcccagg aaatagaaat gaacagccag acaatggaag cacaggattt
    31501 ttaagatatc aagcttgtga taatagaaca aatagtttag gatattggct atgttttata
    31561 gattctagac aaagaaatat agttaggtaa aaataataaa aatgaaaaaa tttagactca
    31621 attcttattt tactgaaaaa attaatgaaa tgtagtacaa aactttaaaa actattgata
    31681 atatttaaat attacactac aatatatctt gaaataccta ttatattatg gaggaaaatt
    31741 atgataaacg agcaaagaat tttaaatgag tttcttgaat tagtacagat agatagttta
    31801 tctttaaaag aggggaatgt tgctaaagta ttagttaaaa aattagaaga aataggttgt
    31861 tcagtagtaa tagacaatgc tggtgaaaaa gcaaatggag aaactggtaa tataatagct
    31921 acattaaaag gaaataaaga aggaaagaaa atattattta gttcacatat ggatacagta
    31981 actccaggta taggggtaaa acctatagtg gatgaagcaa atggaataat aaaaagtgat
    32041 ggtactacaa tattaggttc tgatgataaa gcaggtatag cagctatatt agaaggctta
    32101 agatatataa atgaaaataa catagaacat actgatatac aagttgtatt ctctatatgt
    32161 gaggaatgtg gattagttgg agctaaaaat ttagactatg gaaaaataga ttctgaatat
    32221 gcatttatat tagatagtgg tggttctcca ggtgaaataa tagtaaaagc tcctgcacaa
    32281 gatgtaataa atgttaaggt attaggaaag actgctcatg caggtcttga acctgaagct
    32341 ggaataagtg ctataatggt agctgccaga gcaatagaaa atatgaattt acttagaata
    32401 gacgaagaaa caactgctaa cataggaatt ataaacggtg gaacagctac aaatatagta
    32461 acaggagaag ttaatatagt tgcagaagcc agaagtctaa aagaaaataa attagatgtt
    32521 caaactaaac acatggtaga gacttttgaa aaagctgcga aagattttgg agctcaaatt
    32581 gagatagatg ttaatagagc atacacacca atagatgtta gtgaggattc tgaaataata
    32641 aaacttgcaa agaaggcttt ttcaaactta ggtatagaag gtcatactga atctacagga
    32701 ggaggtagtg atacaaatat attaagtaag aatggtatcg aagctataac tttaggaatt
    32761 ggtatgaaaa atgctcatac actaagtgaa catatagcta taaaagactt atatgattct
    32821 gctaaaatgg tagtggagat aataaaggaa gcataaattt aaataatagt gtataatata
    32881 tatatcgcct agcttaagct aggtgatttt tcattttata gaagattgat aatctctatt
    32941 gaaagcaaag agtatacatt tgcatataag agagtgttta atttatgttc aaatgtaggt
    33001 tcttaataat atttagattg tttcataagg gaggaataaa tatggaaaca aggtatgaag
    33061 aagcaaataa aataacaata caatcaatac tttggaatat agtattaaca ataattaagg
    33121 ttatagctgg tgttattggt aattctagcg ctatgattgc ggatggatta cattcagcat
    33181 cagatattat aagttctata ggagtactaa taggcaatta tgtatcttct agacctggag
    33241 atagagagca taattatggt catgagaaag cagagacatt agtatcattt gtgttatcaa
    33301 tattgcttat tttcgtttct ataacaatag gtatagaggc aattaaatct ttatttaact
    33361 tagatgcatt aagtgttcca tcaatattac cattagtagt atcagttatt tcaatcctga
    33421 taaaagagta tcaatataga ataacaataa aagttgctaa aaagataaat tcaccagccc
    33481 taaaagctga tgcttggcat cacaggtcag atgctttatc ttcagtagct gcttttatag
    33541 gtataggtgg ttctatttta gggtttaaac cattagaccc aattgcgtca gttgtagtgg
    33601 ctatatttgt agctaaagta ggaattagta tacttataag ttctgttaat gaattgatgg
    33661 atgtttcagt tgatgaagaa gagataaaag aattaaaatt tattgtagca gatacagaag
    33721 gtgttaaaaa tcttggtgat ataaagacta gaaaacatgg agctatggca tatgtagatt
    33781 tgactatatg tgtggatgaa aatttgacag ttaagcaagg tcatgatatt gctaccaagc
    33841 ttgaaaaaca tataataaaa catatggaat ttgtaaaagg tataacagtt cacgttgaac
    33901 catgtactaa ttgccaagga aataagtgta ataattaatt aaaacttaaa aaaaagtgta
    33961 aaaagttata taattgtttt atgattataa actctagggg tggttaaatg agcaagaaaa
    34021 gaaaactaaa gaaaaaagct gcaatattaa taggagtagt tgttttcttt ataatatact
    34081 ttatatcctt tagaatagga ataaatatgg aaaaggataa aagtgaagtg ccaaagaagg
    34141 tagaaaaaga agaagaagat aataggtctt taaggactca aataaaagag aagaagaagg
    34201 tttatatatc agataaaaat atagaaaaca taaaaattga agaagatatg tgggatgaaa
    34261 taaagttatt tttctcagat tttaagaaag taagaaatac tacttcttct tacgaagcag
    34321 tttatgaagg ttattctgat gatggagtta aattttctac tgaccttaac ttttttagag
    34381 tatatactgt aaataaagaa gaatattata aagtaccagt tagttctaag gatgctttta
    34441 agaaactttt agatgagagt atatatactt cgtttgacct tttaagtcat tataaagatt
    34501 ggaaagaggt tactataact catggaaaag aaattaaaaa agttcataga tggaaatttg
    34561 atgatttagc acataagatg atttcaaaga gggttgtagg aaaagttcaa cctgagaaaa
    34621 gtaaggaaag aagtgactat aattttacta taaatataaa aggtgataat tttgatgtaa
    34681 aagttgatac aatgggaaaa gattatgtaa aagttacttc taagaaaaat gtatcttact
    34741 atgaggttca tacaagttta tttgattacc ttaaaaatga gatatttgaa attggaaaga
    34801 aataagactt taagaaggct atcaaattag atatttgata gcctaaatta ttttatatat
    34861 tatttttcaa ccaacaagtc tgcaacctta taaattggac cagcaccagc agttattact
    34921 aaatcattat ccttaacatt ttcacgtaag tattttacta tatcttcaaa ttcttttata
    34981 tagatggcat ctacattgtt ttgatataat ttctcaacca agtccttaga atgtatatct
    35041 cctgggtctt tttccctagc agcatatata tctgttatga taactttatc agcagcatag
    35101 aatgcctctg aaaattcacc taaaagagat tttgttctag tataagtatg tggctggaag
    35161 atacaccata aagttgattt ttttaatttt ttggcagcag ctaaagttgc ttttaactca
    35221 gttggatgat gtgcataatc atctactact aaagcattct tatagtaccc tttagtttca
    35281 aatcttcttc cgacaccttt ataaattttt atattttttc ttatagtctc taagtcaata
    35341 ccagaaacaa gagctgccat tatggcagaa gaagcattgt atatgttatg aagaccatat
    35401 acagaaagtt caaattctcc taaatctctt ccttcatatt ctattctaaa tattccatga
    35461 ccactattgt caaagtgaat atcttttatt acagcatcat tgtcagaatc tctgccgtat
    35521 tttataatag tagctttaac atcatacaat atgtcatcaa tattttcatc atcgccattt
    35581 attatgaaat agccttctgg gggtaataat tttccaaatt tgttaaaaga agccttgatt
    35641 tcatctattc cagagaaata atcaagatgg tcttcttcaa catttaaaac tatagatatc
    35701 tttgggttaa aattaaggaa actatctaca tattcacatg cttcagttat aaagtggttg
    35761 gaattaccta tcttaacatt accacctatc atacttaagt taccaccaac tagtatagtt
    35821 gggtctaagt ctgcatattc aaagattgta gataacatag atgttgtaga agtttttcca
    35881 tgtgttccag aaactgctat agaattttta tattccctca tgatttggcc taaaaaagtt
    35941 gctctgttca ttaccaattt atttttttct ttagcagcca caagttcttc attgtctgga
    36001 tgaactgctg ctgtataaac aaccatattt acatcatctg atatattatc ttttttctga
    36061 ccaatataaa tagttgcccc ttggtcccta agtttgtcta ataggtatga ttcgtttgaa
    36121 tccgaacctg aaacttgata acctttattg atacatattt cagctaatgc actcatgctg
    36181 ataccgccaa ttcctataaa gtgtatattc ataataaaac cacctttctt tttaattaag
    36241 tcctacaata tgaactattt ctgtggtata ataatataat gttcaatatt ttataattaa
    36301 tatgcatata atattatatg tattttattt ttgaatataa taaaacataa gtaatattat
    36361 accacaataa gaattgttat gcattagtta gtttaacgtt atataaaggt acttgtagga
    36421 ggaaataata atgaaattta aaagaacaga gagaattgga gcaatagtta aaatactgtc
    36481 tgataatcca aataaaatat atactttaag ttattttaca aatcaattta atgcagcaaa
    36541 atcaacaata agtgaagatt tattagttgt aaaaaatgta tttgagaagc tacatttagg
    36601 taaagttata actatttcag gagcagcagg aggagtaaaa tatataccta aaacttctat
    36661 agcagaaaat gcagagttct taatggaatt atgcgagaga atatgtgaca agtctagaat
    36721 actatctggt ggttttttat acttgattga cctaatttac gaccctacaa tagctgctaa
    36781 gataggaaag atattcgcat caaatattga gtatgtagat gcagattatg ttgtgacaat
    36841 ggaaacaaaa ggtataccta tggcacttat gactgcaaaa gctatgaatt tacctctagt
    36901 tataataaga aaagatataa aggtatcaga aggtcctaca cttagcatga catatgtgag
    36961 tggaaacagt tctaaagttg aaagtatgag tttgccaaga aaagctctta aaccagatag
    37021 taaagttata attatagatg atttcatgag gggtggaggt actataaaag gaatggtaga
    37081 ccttatgaat gaatttggtg cagaggttat aggtactgga gtattcatat ctacaacgaa
    37141 tccttcagaa aaaatggtaa aagattatat ttcattgatt caattggatg ttaatggaga
    37201 taaaatagta gtagaaccaa acttaaagac ttttaaagat gaatatagaa atgaagattt
    37261 agacgaagaa gaagattcag aatttgaatt tgagatagat gaagattaat aaaaaaaata
    37321 tttagaaaaa tatgtaaaaa taaaaagagg atatccctag ttgttcatag aattatttaa
    37381 aaaacagttt atattaattt attccagcga taataatttt aatataaatt aatttctgtc
    37441 aaaatcaaag ggggatattt tggtatgaag ataactgacg taagagtaag aaaattaaca
    37501 gaagaaggta agatgaaatg tatagtttca ataactttcg ataatttgtt tgtagtgcat
    37561 gatataaaag ttatagaagg ccataatggg ctatttatag caatgcctag cagaaaagta
    37621 ggcgaaggaa atttcagaga tatagctcat ccaataaatg cagaaatgag acaagtttta
    37681 gaagatgcag ttttacaagc gtatcatgaa gcattagttc aatgggaagt agctgctgaa
    37741 taataataaa tatataaaat ggagagagcc cgaacattta ggctcttttt aaatgtagaa
    37801 tttctattaa aatatgtact ttttagtaaa aaagtgctat attattagtg ataatgtata
    37861 tgttgtatac aaattgttag ataaatgtat tataatgtat attgattaag agaataatac
    37921 gaataaaatt aaattagatt gaatggagtg tatcaatgaa ctttaaagct ataatccttg
    37981 ctgctggcaa aggaacaaga atgaagtcta aatacccaaa agttatacat aaagtatgtg
    38041 gaaaagaaat ggttaaccat attatagatg tttcaaaaaa atcaggggta aaagatactg
    38101 tcgtgatttt aggacatgaa gctgatgtag ttaaagaaaa gcttgcagaa gaaattataa
    38161 tagccatgca aacagaacaa cttgggacag gacatgctgt aaagatggct aaagaatata
    38221 ttaatgatga agatacaata gttgtacttt gtggagatac tccacttata aaagaagaga
    38281 cactaaagag actatttgaa tatcatatag aaaataaata tcatgctaca gttcttacaa
    38341 caagagttgg aaatccaaca ggttatggta gaataataag agataagaag ggtgaccttc
    38401 ttaaaatagt ggaacaaaaa gatgcaaatt cagaagaaaa gatgataagt gagataaact
    38461 ctggaattta ttgttttaat ggaaagagtc ttagagaagc actagatttg ctaaataata
    38521 acaattcaca aggtgaatat tatttaactg atactgctaa aataatgaga gataaaggat
    38581 taaaagttgg tgcatttgca ggttcaacta ttgaagaact tatgggcgtt aattcaagag
    38641 tagaattatc aaaggcagaa gaaattatga gacgaagaat aaatgaatct catatggtaa
    38701 atggagtaac tataatagac actaactcta cctatataga atctgatgtt atgataggca
    38761 atgatactat aatatatcca ggtgttatgt tacaaggaaa aacaaggata ggttcagatt
    38821 gtataattgg aatgaattca tctataacaa attcagaaat aggtgatggt acagaaataa
    38881 aaaattcaac tataattgat agtaaggttg gagaaaattc tactgttggg ccatatgcat
    38941 acttaagacc aaagagtgac ttaggaaata atgtaaaaat aggcgatttt gtagaagtaa
    39001 aaaatgctat tatagaagat ggctcaaaag catctcacct ttcttatata ggagatgcac
    39061 atgtcggtaa aaatgtaaat attggatgtg gagtagtatt tgtaaactat gatggtaaaa
    39121 ataaatttaa atcaatagta aaagacaatg cgtttatagg ttcaaattct aatttagttg
    39181 caccagtagt tgtagaagaa aaaggatata tagcaacagg ttcaacaata acacatgatg
    39241 tcccagatgg tgctttggca atagcaagag aaagacaagt tatcaaagaa ggatgggtag
    39301 aaaaaaagaa tcaaaaagac gaccaaagta agtaattatg tagtttatta ttttggctaa
    39361 aaaatttagc tcgaaattat tataaattat attcattagt attttaaaat tttcaggagg
    39421 gttacataaa tgaatactag cggaagcgag attaaaatta ttgcaggtaa ttcatcgaaa
    39481 gagttagcac aaaaaatagc tgactatata ggtgtatcag tactagattg tgaagtaggt
    39541 acatttagtg atggtgaaat atgtgtaaac atgaatgaga cagtaagagg ttgtgatgtg
    39601 tttgtagtgc aatctactaa cagcccagta aatgataact taatggagtt attaatctta
    39661 attgatgcgt taaagagagc atcagcaggt agaataacag cagttattcc ttactatgga
    39721 tatgcaagac aagacagaaa ggctaaggca agagatccaa tcactgctaa attagttgca
    39781 aatttaataa ctgctgctgg agcagataga gtattaacaa tggatttaca tgcagctcaa
    39841 atacaaggat attttgatat accattagac cacttactag gtggaacaat attagcaaat
    39901 tattttaatg aaaagaaaat agaagattta gtagtagtat ctcctgactt aggaagtgtt
    39961 actagatcaa gaaaatttgc taatacttta aatggagaag ttccaatagc tataatagat
    40021 aaaagaagac cgaaagctaa tgtttgcgaa gttatgaact taataggtga tgttaaaggt
    40081 aaaaatgtta tactattaga cgatatgata gatacagcag gaacaatagt taatgctgca
    40141 aatgcactta aagagtttgg agcaaaagat gtttatgctt gttgtactca tggtgtgtta
    40201 tcaggtccag ctattgaaag aattgctaat tcagaaataa gtgagttaat agttcttgat
    40261 actatacaac ttcctgaaga gaaaagaata gataagataa agataaaaac agtagctcct
    40321 ctatttggag atgctataag aatgatattc tctaatgaat cagtaagtaa attattctaa
    40381 tttattgtat ttatgagagt tgtttaaaag gatataaacc ttttgagcaa ctcttttttg
    40441 attttatact tatggaatgt tatttttttc ttgttagaaa ggtatatatt attgtaaaaa
    40501 gagattatag tttaaaatta tgtatattta agtaaaataa acctaataaa gggtatatta
    40561 ttcaaaatat tatgaatata aaaaatatta caaaaaagta ttgaaaaaat tcagattatg
    40621 tagtattatg taaatataaa taaagaaaga ggtgtttcca atgggaaggt tagaagatga
    40681 acccctaata attaaatgat tcagtcacaa tctaaagtga atataaaaat ctattgaaag
    40741 tttatttata agaattgaaa atagtatcta ttctatcttt agtgtttgtg agagggttct
    40801 ggattatata agattactgt aaagagtaat tttgcacaat aagcatatct gtaaatgaag
    40861 agtttcgttt atataatgat tgtggctgaa tcataactat aatagataga atgtttataa
    40921 tagatgattt tataaaatac tatattgtaa tagcttttag ctttcaaata aatattagca
    40981 aaggatggaa atgacaatga taaaactaaa tgataattta taaatatctc cttacgatat
    41041 ataataattg tagggagatg tttctttttt tttgtatagc ataatttttc tagaaattct
    41101 gtttcaatat gtaaaaagtt ggtatatata aatagtataa acagtgttgc taaaatatgg
    41161 tagatattat ataatataag tggatatgta tatacattat gagttatata atttgaattt
    41221 aataaaatag aaacgttgat ttatgttctg taatgtgggc accttggagc atattgagtt
    41281 agtggtgcaa ccggctatga aattgtattt atttataaat actattattt tcatagcttt
    41341 ttttatttaa aaaatggtta tatgttagaa ataaatttgg ttagtacgtt taaataattt
    41401 tacttagatt tttaaccaga tatattgtaa gtaagtatta attaaaaaat ttaaggggga
    41461 ataaaaatga agttaaaaaa gaataaaaaa ggtttcactt tagtggaatt attggtagta
    41521 attgcaatta taggtatatt agcagtagtg gcagttccag ctttatttag taatataaac
    41581 aaggctaagg tagcaagtgt tgagtctgat tatagctcag ttaagagtgc agcattatct
    41641 tattattcag atactaataa gataccagtt acaacaactg ataaagctgg tttaactatt
    41701 ttagagactt atatggagtc tttgcctgat aaagctgata taggtggaaa atataaattg
    41761 attaaagttg gtaataaatt ggtattacag ataggtacag atactgaagg agttacatta
    41821 acagaagcac aatcagcaaa attattgagt gatataggta aagataaaat atatacaggt
    41881 gttacaggag ataattttgg agacgaatta acagatacta caaaaataga taataaagct
    41941 ctatatatag tacttataga taatactgtg atggactcaa caaaataatt aaaactatat
    42001 ttttataaag aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    42061 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    42121 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    42181 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    42241 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    42301 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    42361 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    42421 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    42481 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    42541 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    42601 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    42661 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    42721 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    42781 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    42841 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    42901 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    42961 gatagctatg agggttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    43021 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    43081 ggttgttggc cctactggaa gtgggaaaac cactacattg tattcaatgt taaatcaact
    43141 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    43201 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    43261 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    43321 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    43381 tacagcagtt ggagcaattt cacgtctttt agatatggat gtagaaagtt ttttaataac
    43441 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    43501 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    43561 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    43621 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    43681 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    43741 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    43801 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    43861 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    43921 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    43981 ttatctagta gaaagagaaa aaaagacttg ggagctgacc agataagcca cttttgcaga
    44041 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    44101 agaaggtcaa ccaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    44161 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    44221 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    44281 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    44341 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    44401 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    44461 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    44521 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    44581 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    44641 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    44701 tatattatag atagtgtaaa taacactatg gcagagaaga ttttagcatc agctaaagat
    44761 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    44821 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    44881 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    44941 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    45001 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    45061 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    45121 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    45181 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    45241 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    45301 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    45361 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    45421 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    45481 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    45541 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    45601 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    45661 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    45721 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    45781 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    45841 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    45901 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    45961 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    46021 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    46081 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    46141 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    46201 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    46261 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    46321 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    46381 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    46441 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    46501 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    46561 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    46621 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    46681 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    46741 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    46801 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    46861 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    46921 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    46981 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    47041 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    47101 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    47161 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    47221 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    47281 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    47341 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    47401 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    47461 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    47521 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    47581 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    47641 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    47701 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    47761 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    47821 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    47881 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    47941 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    48001 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    48061 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    48121 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    48181 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    48241 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    48301 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    48361 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    48421 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    48481 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    48541 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    48601 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    48661 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    48721 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    48781 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    48841 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    48901 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    48961 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    49021 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    49081 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    49141 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    49201 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    49261 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    49321 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    49381 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    49441 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    49501 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    49561 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    49621 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    49681 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    49741 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    49801 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    49861 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    49921 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    49981 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    50041 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    50101 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    50161 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    50221 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    50281 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    50341 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    50401 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    50461 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    50521 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    50581 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    50641 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    50701 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    50761 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    50821 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    50881 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    50941 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    51001 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    51061 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    51121 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    51181 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    51241 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    51301 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    51361 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    51421 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    51481 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    51541 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    51601 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    51661 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    51721 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    51781 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    51841 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    51901 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    51961 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    52021 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    52081 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    52141 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    52201 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    52261 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    52321 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    52381 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    52441 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    52501 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    52561 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    52621 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    52681 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    52741 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    52801 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    52861 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    52921 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    52981 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    53041 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    53101 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    53161 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    53221 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    53281 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    53341 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    53401 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    53461 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    53521 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    53581 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    53641 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    53701 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    53761 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    53821 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    53881 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    53941 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    54001 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    54061 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    54121 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    54181 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    54241 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    54301 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    54361 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    54421 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    54481 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    54541 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    54601 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    54661 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    54721 ttaaactcgt tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    54781 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    54841 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    54901 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ctatttctaa agatagagat
    54961 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    55021 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    55081 gcgtctaaga ctagcaataa aaaagttaaa aaaggaaaga aaatagaatc ctttttagat
    55141 ttaagtgttg gagactatgt agttcatgaa aatagtggtg taggtagata cacagggatt
    55201 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    55261 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    55321 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    55381 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    55441 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    55501 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    55561 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    55621 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    55681 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    55741 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    55801 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    55861 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    55921 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    55981 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    56041 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    56101 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    56161 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    56221 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    56281 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    56341 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    56401 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    56461 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    56521 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    56581 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    56641 aaaatgctta atgatgcaat taaaaaagtc aaaggtgaac ctattgtgga ggaaattgat
    56701 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    56761 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    56821 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    56881 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    56941 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    57001 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    57061 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    57121 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    57181 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    57241 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    57301 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    57361 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    57421 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    57481 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    57541 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    57601 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    57661 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    57721 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    57781 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    57841 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    57901 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgacgat
    57961 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    58021 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    58081 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    58141 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    58201 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    58261 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    58321 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    58381 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    58441 gattctataa tagctgtatc taagcttcct aaaaaagatt acaaagaaaa aagtataagt
    58501 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    58561 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    58621 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    58681 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    58741 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    58801 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    58861 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    58921 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    58981 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    59041 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    59101 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    59161 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    59221 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    59281 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    59341 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggcc tacttgctta
    59401 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    59461 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    59521 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    59581 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    59641 tagataatgt tattgtaata agaagactta tggaagcagg atttacatac aaagtggcaa
    59701 attcaatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    59761 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    59821 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    59881 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    59941 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    60001 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    60061 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    60121 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    60181 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    60241 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    60301 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    60361 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    60421 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    60481 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    60541 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    60601 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    60661 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    60721 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    60781 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    60841 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    60901 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    60961 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    61021 tcatatatag acttggatac aagcatgata ataactcagg tatatgataa atttatagca
    61081 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    61141 aatggagcag gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    61201 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    61261 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    61321 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    61381 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    61441 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    61501 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    61561 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    61621 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    61681 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    61741 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    61801 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    61861 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    61921 ataaatagat ttgactttat agaaaaaagt gcaatgaact tgaataaaaa attagaagac
    61981 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    62041 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    62101 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    62161 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    62221 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    62281 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    62341 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    62401 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    62461 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    62521 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    62581 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    62641 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    62701 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    62761 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    62821 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    62881 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    62941 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    63001 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    63061 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    63121 aagagggaat tttaaattca tcaattattt tgctataatc tttgatgtat tgttttggtt
    63181 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    63241 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    63301 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    63361 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    63421 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    63481 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    63541 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    63601 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    63661 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    63721 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    63781 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    63841 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    63901 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    63961 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    64021 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    64081 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    64141 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatgggaac
    64201 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    64261 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    64321 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    64381 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    64441 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    64501 tatgagttca tttatatatg gtgagattga caaaacgtta aatcatataa aagacagagg
    64561 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    64621 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    64681 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    64741 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    64801 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    64861 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    64921 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    64981 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    65041 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    65101 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    65161 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    65221 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    65281 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    65341 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    65401 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    65461 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    65521 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    65581 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc taccatggga
    65641 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    65701 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    65761 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    65821 attacttcta atatgatgca attaagagat atactattag gtagtttgat agtactagta
    65881 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    65941 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    66001 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    66061 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    66121 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    66181 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    66241 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    66301 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    66361 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    66421 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    66481 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    66541 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    66601 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    66661 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    66721 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    66781 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    66841 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    66901 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    66961 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    67021 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    67081 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    67141 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    67201 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    67261 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    67321 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    67381 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    67441 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    67501 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    67561 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    67621 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    67681 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    67741 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    67801 acagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    67861 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    67921 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    67981 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    68041 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    68101 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    68161 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    68221 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    68281 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    68341 ttccaagcca agtgtatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    68401 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    68461 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    68521 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    68581 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    68641 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    68701 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    68761 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    68821 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    68881 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    68941 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    69001 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    69061 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    69121 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    69181 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    69241 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    69301 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    69361 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    69421 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    69481 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    69541 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    69601 aatccacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    69661 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    69721 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    69781 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    69841 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    69901 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    69961 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    70021 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    70081 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    70141 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    70201 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    70261 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    70321 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    70381 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    70441 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    70501 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    70561 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    70621 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    70681 tgatgtatgg tggcaaaatc aagtcagaaa aaaattaata gaataatagc tgttgggatt
    70741 gtgggaatcc tcgtaataac gagtataaca gttgttttga gtatgattgc aactatgtaa
    70801 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    70861 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    70921 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    70981 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    71041 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    71101 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    71161 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    71221 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    71281 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    71341 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    71401 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    71461 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa
    71521 gaaatgacta taataagaca acgagtgtaa atgatagcaa taagtcatca aatatatcta
    71581 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    71641 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    71701 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    71761 acgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    71821 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    71881 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    71941 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    72001 atttaatttc acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg
    72061 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    72121 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    72181 ttgaaggaga cgttatatat tctacttttg accaagtatc aagtcatcat gtcaaggttg
    72241 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    72301 tactagatag tataactaga ttggcaaggg catataacct tacaatatct cctacaggta
    72361 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    72421 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    72481 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    72541 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    72601 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    72661 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    72721 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    72781 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    72841 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    72901 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    72961 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    73021 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    73081 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    73141 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    73201 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    73261 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    73321 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    73381 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    73441 aggttctttt gttatgatag atacaatgat tcaaggcata aaaagcttaa acttttcttc
    73501 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    73561 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    73621 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    73681 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    73741 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    73801 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    73861 atttaaaaga ttacatccta gatgtggtac gaatttccta tttatagtta tggctgtatc
    73921 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    73981 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    74041 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    74101 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    74161 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    74221 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    74281 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    74341 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    74401 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    74461 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    74521 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    74581 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    74641 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    74701 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    74761 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    74821 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    74881 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    74941 aatattatga aaagtaatgg atataaaaaa atatatacaa agaaggatat tcaaggcatt
    75001 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    75061 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    75121 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    75181 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    75241 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    75301 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    75361 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    75421 tggagctgga ggagatgaag cagctctatt tgcgggtgat ttatttagaa tgtactcaag
    75481 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    75541 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    75601 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    75661 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    75721 tccaaatgat ttaagaatag atgttttccg ttcttcgggt aatggaggac aaagtgtcaa
    75781 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    75841 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    75901 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    75961 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    76021 tgaccataga ataaatttaa ctttatataa gctagatgca ttcttagatg gagatataga
    76081 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    76141 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    76201 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    76261 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    76321 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    76381 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    76441 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    76501 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    76561 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    76621 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    76681 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    76741 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    76801 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    76861 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    76921 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    76981 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    77041 aaaacttctt agagaaggta aaacagttat ttttccaact gagacagtat atgggttggg
    77101 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    77161 tgacaatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    77221 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    77281 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    77341 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    77401 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    77461 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    77521 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    77581 taaagaagtt ttagaggaac ttcttggaga ggtaaagctt gacccatcat taagtaaaaa
    77641 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    77701 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    77761 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga
    77821 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    77881 gtttgatgct cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt
    77941 ccctaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    78001 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    78061 atacttgtag aagtcctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    78121 gaagtattga agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg
    78181 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    78241 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    78301 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    78361 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    78421 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    78481 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    78541 aaaaaagaaa taataagcta tttagaaagt aaaggtatag aatgtgtaga ttatggaact
    78601 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    78661 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    78721 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    78781 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    78841 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    78901 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    78961 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    79021 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    79081 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    79141 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    79201 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa
    79261 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    79321 ttgaatatta ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    79381 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    79441 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    79501 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    79561 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    79621 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    79681 aggttctttt ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga
    79741 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
    79801 aatagtagtc tattttaatg tggcatgtta gttgcttacg attataggta ttaagtgtat
    79861 tagttaaatt gagaattgaa tattaataat attattggga gtggtaatac atgaagaatt
    79921 gtatagtagt aagaggggct ggagatttag caacagcagt tattcataaa ttacacaaca
    79981 gtggttttaa agtagttgcg ttagaaatag aagagccatt agctataaga aggcaaattt
    80041 ctttttgtga ggctgtttat gaaaaaaaag taactgttga aggtgtagaa tgtatatttt
    80101 gcaatactga aaaagaaata gaggaagttt tatctcaaca taaagtagcc ttgattatag
    80161 acccaaaagg agaaaaatta tctaaattaa atccaatcgt tttggttgat ggaatactcg
    80221 ctaagaaaaa cttaggtaca actaaagata tggcaccact tacaatagct ttaggtcctg
    80281 gattttgtgc aggagaagat gtagatgttg ttatagagac aatgagaggt cataatctag
    80341 gaagaataat aactaatggt agagcagaaa aaaatacagg agtaccagga gcaataaaag
    80401 gtttttctaa agagagagtt atgcattcac agtatgatgg cataatggaa aatgtatgta
    80461 agataggtga tatagttgaa aagggagacc tactttgcta tattaataat tatgaaaaaa
    80521 aacatgaagt tagagcaaca atatctggag tccttagagg tttattaaag gatggagcaa
    80581 aggtaaacaa aaatttaaaa atactagaca tagaccctag aaaagaagaa gttaagaatg
    80641 cttttacaat atcagataaa gctagatgta tagctggagg ggttttagaa gccatactaa
    80701 acaatgggat ttatccccaa aattaattta tattagataa agctattaat attttattaa
    80761 gatgatataa taaatttaat agattaaaga gagcatatga cgggtattta aaatttaaat
    80821 ggtaaaaatg ctcttttttt attggacatt caattaacat gtgtatataa tataaaaata
    80881 agtatttatt taggttgaaa tctaataaat atataatacc agtacataag caaagaggag
    80941 aaaagtaaat tatgagacca tcatgggatg aatacttcat ggaaattgca gaagtagtga
    81001 aaaagcgttc aacttgtatt agaagacaag taggagctgt aattgtaaga gacaaacaaa
    81061 tattaacaac tggatataat ggttctccga gaaatcttga acactgtgaa aatattggat
    81121 gtaagaggca ggaattaaat attccctcag gagaaaggca tgaattatgc agagcattac
    81181 atgctgaaca aaatgcgata atacaagctg ctcataatgg tattagtgta gatggggcaa
    81241 ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa aatgtgcata aatgcaggta
    81301 tattgaaaat tgtttatgaa ggtgattatc cagatgatat gtctacagaa ttattaaaag
    81361 aagcaggaat agaactcata aaattttagt ggataaaatt tataagacaa taaatgtcaa
    81421 attatgacac tgaaatacaa tggtatcaaa tgattaaaag aaagtatttg aaaaatcatc
    81481 agattagtag taagatatat tttataatta aataaattta tgagatagat taattacatg
    81541 ttatttattt tataataatg agggatacta tttatgagta aaagaaatac acatacagaa
    81601 attgcaaagt tgtttagttt aataagtcaa ataggattga tgattgtaat atcaatatta
    81661 ggatgtactt tcttaggaaa atttttagat agtaaattga atactactcc agttctgaca
    81721 ataatatttt tattgttggg tgttggaggt gcttttagtg gtgtttataa aactctaata
    81781 gtatatacac gaaggaagtg atatattgga tataaaatta cttaaccagg taaggtcagt
    81841 aacaaaaggt atagtaatat ttgatgtagt tttaattgtg ctaatgttga taacatcaac
    81901 attaagtaaa ccagtattat taggacttat atttggctct ataatagcta tgttaaattt
    81961 tagattattg gcgatatcac tggaaaaatc agtgacattt cctgctggga aggcacaagc
    82021 ttatgcatca gcacaatatg ctgttagatt atttataatg gcagttgttc taattgcatc
    82081 agctacagta ccacatttaa atataatcgg aacagcattg ggattattga gcacaaagtt
    82141 tgtcatatta tcaaaaaact ttataaataa attaaagagg aaggaggcat aagtatgaat
    82201 caagcgcatt attatctcat aggaaacttt aagattagtg aaacaatact agtaagttgg
    82261 ggaataatgg cctttttagc gatagcatct tattttttga ctagaaactt aaaaaaagtt
    82321 ccaactagta aagttcaaat attcttagaa tttgcagtag gtggtcttgc taaaatggtt
    82381 acagatgtta tgggtaaaaa tacagtaaaa agaatgccat atatgatacc atacatagga
    82441 agcttgtttt tatttttcgc atgttcaaat ttaagcccat tacttggatt aaggtcacca
    82501 acaaccgact tagacacaac tgctgcatgg gcaatgatta cattttttat gatttatttt
    82561 gcaggagtta aatttaaggg gttatcatac tttaaagaat tggctcaacc acttgccata
    82621 atgttaccaa tcaatattat aggtgagtta gctagaccaa tatcattaag tttccgtcca
    82681 tttgggaata ttttaggtgg aacaataata atgggtctgt tttatcaact tttaggtttt
    82741 atatctagtt taataccagg attatcaata ccaattggag aaatattaat accagtccca
    82801 ttacacttgt actttgactt atttgcagga ttgttacaag catatatatt tataatgtta
    82861 acaatggtat ttatatcaag tgcaggagag ttagattaag aaatcaactt atattaagat
    82921 aaaaataaaa taatttaaaa aattaaagtt ttaaggagga aataattatg gaaacagcaa
    82981 tagtagcggc agcatcagca ataggagcag ggatagcagt tgcaacaggt ataggtgcag
    83041 gtataggtca aggtatagca gcagctaaag ctgcagaagc agtaggaaac cagccagaag
    83101 ctaaaggtga tataacatca actttattac taggagttgc tatagcagag tcttcagcaa
    83161 tatatggttt ggttatatca ataatacttc tattcgtaaa tccattcttt aaatacttag
    83221 gaatgtagat ttagtcctta aatattttga gataggttta taggtaaatc atctcaaact
    83281 tataatcatg agatgaactt tatcatctcg tgattataag tagttatgat ttatggaaag
    83341 gaggataatt atggaaaaag cactagtagg tataacgtgg gaatttgtgt tccaaattgt
    83401 taatactttt ataatatttt tacttctgag aaagctttta ttcaagccag tattaaatat
    83461 aattgaatca agagaaaatg atattaaaag tgatttggca gaaggcgaaa aagcaaaaaa
    83521 tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc aattttgcca aagatgaagg
    83581 tcaagaaatc ataaaacaag caactataag agcagagcag aaatcagatg atatagtaaa
    83641 tactgcaaag aaagatgctc ttgatataaa agaaaaagca aacaaagata tagaacaaga
    83701 aagacaaaag gttataaatg aaatcaaaaa tgatatatct aacatagcac tacttgcagc
    83761 atctaaagtt attgaaaaag atttagataa atctaaacat gaagaattga tagaaaactt
    83821 tataaaagag gtaggggaag ctaaatgata aatgtaatag caaatagata tgccgaagcc
    83881 ctatttcaat taggagaaga agaaaattca actgatgtct tattcaaaga actagaaaaa
    83941 gtagtagata tgatgactaa agttagtaaa gatttctaca aagttttaaa atcaccatta
    84001 gtttcaaaat cagaaaagaa aaacttggta gaaattattt ttagtaagga agtaagttct
    84061 aacataaaaa actttttaaa agttttagta gataaagata gaatatcata tttagaagat
    84121 atcgaattag cttataaaga attattaaat aagaaaaaca atgtaattga tggagtagca
    84181 ataagtgcta ttccaatgag tgaaacagat attaaagaac ttgaagtcaa actttcaaat
    84241 aaatacaaca aaaatgttac tatagaaaat gttgttgaca agactatact agggggagtt
    84301 ctagttagaa ttggaaatga acaaatagat ggcactgtga aaactcgtct tgataagatg
    84361 aaagaaaagc tatctgaagt aatatcttaa gaatggcggt gaacccatga acttaaaacc
    84421 tgaagaaata agttctataa ttaaacagca aataaaaaat tatgagaata aagttgagtt
    84481 gacagataca ggtagtgttt taacagttgg ggatggtata gcgagtgtat atggattaga
    84541 aaaagctatg tctggtgagt tgctagagtt cccaggtgaa atatatggta tggcacttaa
    84601 cttggaagaa gaagtagttg gagctgttat ccttggagat gacagtgaaa taaaagaagg
    84661 cgatatagtt aagagaactg gaagaatagt tgaagttcca gttggagaag ctttaatagg
    84721 tagagttgta aactcactag gtcagccaat agatggaaaa ggacctatag cgtacacaaa
    84781 aactagacca gttgaatctg aggcacctgg tataatagat agaaggtctg tttatgaacc
    84841 attacaaaca ggaatcaaat caatagattc aatgatacca attggtagag gtcaaagaga
    84901 gttaataata ggtgacagac aaactggtaa aacatctata gttattgaca ctatactaaa
    84961 tcaaaaagga aaagatgtta tctgtatata tgtagcaatt ggacaaaaac gttctactat
    85021 agctcaatta gttagctctt tagaaaaagg tggagctttg gattatacaa tagtagtttc
    85081 agcaacagca tctgaatcag caccacttca atatatagca ccatatgctg gagcagctat
    85141 gggtgaagaa tttatgtaca atgggaaaca tgtacttata gtgtatgatg atttaagtaa
    85201 acaagctgtt gcatatagag aaatgtcatt gttacttcga agaccaccag gacgtgaggc
    85261 atatcctgga gatgtattct atttacattc aagattactt gaaagagcag ctaagttatc
    85321 tgatgaattg ggtggaggtt caatgactgc cttgcctata atagaaactc aagctggtga
    85381 cgtttctgca tatataccaa caaatgttat atctataaca gatggtcaaa tatacttaca
    85441 accagagtta ttctattcag gtgtaagacc agcagttgac cctggtatat cagtatcaag
    85501 ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt gcaggaacat taaaacttgc
    85561 atattcacaa tatagagaac ttgcagcatt ctcacagttt ggttctgact tagatgaaga
    85621 tactaaaaag agacttgctc aaggtgaaag aatcgttgag atattaaaac aaggtgagca
    85681 tcaaccaata aaagttgaaa atcaagttat gataatatat gctgttataa ataatcattt
    85741 agaggatatt ccgatagata atatagctag atttgaatca gaattgtatg catttgtaga
    85801 taataattat ccggaaatat caagaaaaat attaggtgga gaagacttca ctcatgattt
    85861 aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt gaagtataat cctttttaaa
    85921 aaaggaggta aattaattgg caggagctgg aatcaaagca atcaaaacac gtatgggaac
    85981 tgttgaaagt acaaagcaaa taactaaagc aatggaatta gttgcatctt ctaagcttag
    86041 aaaagcaaaa gaaaaggctg aagattcaag agaatatttt aatactttat acaatacagt
    86101 aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa tttttaaaag aaagagaagt
    86161 aaaaaataaa tgctatgtag tagtagctgg agacagagga cttgcaggtg gatacaattc
    86221 taatgtatta aaggaatctg tctctcatat gaaaggtaaa aaggaaacag tgattactgt
    86281 tggaaagaaa gcacatgaat ttttttctaa aagaaactac aatatagcaa aagatataaa
    86341 tagtgttgaa gcttgtggat atgaagatgc gacagaaatt tcacaaacta ttattgacct
    86401 gtataaaaaa ggtgaagtag atgaagttta tattgcttat acaaaattta aatctgcttt
    86461 atcacaagaa gttgaaattt tgaaattgct tccattagtc tttgaaaaag atacagacga
    86521 acaaggaatg gcagtaaaag aagaaaaaca aaatagatca aaagtacaat atataccatc
    86581 agcagaagca gttttaagtt atattgtacc tcagtttata tcaggtacag tatatggtgg
    86641 aattttggaa tcttttgcat cagagcaagg tgctagaaga actgcaatgg aatcagccac
    86701 agacaatgcg aatgaaatgc tatctaattt agagttaagt tataacagag caagacagtc
    86761 agctgtaact caagaaataa cagagatagt tggtggagtt gaagctctaa aatagggagg
    86821 ttaggaaatg gcaaatgtag gtaaagtagt acagattgta ggagctgtac tagatgttaa
    86881 gtttgatagt gaacaaagcc tacctaactt attaaatgcg ttagtaataa agttaggaga
    86941 taaagaaata gttgcagaag ttgctcaaca tataggtgat gatactgtaa gatgtatagc
    87001 tatgagtgca acagacggac ttgtaagagg tatggaagtt gtagatacag gagggccaat
    87061 aagtgttcct gttggagatg aaactttagg tagaatattc aatgttcttg gaaaaccagt
    87121 tgatgggaaa ccagctccaa aatctgcacc taaattacct atacatagac cagcaccggc
    87181 gtatgatgaa ctagaaacta cagcagagat actagaaact ggtattaagg tagttgactt
    87241 actagctcca tatctaaaag gtggaaaaat aggtctattt ggaggagcag gagtaggaaa
    87301 gacagttcta atacaagagc ttataaacaa tattgctaag caacatggtg gtatttctgt
    87361 attttcagga gtaggagaaa gaacaagaga aggtaacgac ctttatggcg aaatgagtga
    87421 gtctggagtt ataaataaaa cagctctagt atttggtcaa atgaatgaac cacctggagc
    87481 gagaatgaga gttgctttaa ctggacttac aatggcagaa cattttagag atgagcaagg
    87541 acaagacgtt ttacttttcg ttgataatat attccgtttc acacaagctg gttcagaagt
    87601 ttcagcactt ctaggacgta tgccatcagc tgttggttat cagccaacat tagctactga
    87661 aatgggtgca cttcaagaga gaataacatc aactaagaaa ggttcaataa catctgttca
    87721 agcagtatat gtaccagcag atgacttaac tgacccagca ccagctacaa cattctctca
    87781 cttagatgca aaaacagttt tatcaagaca aatatcatca ctgggtatat atccagcagt
    87841 tgaccctctt gaatctacat caaggattct tgaccctagt atagtaggaa aagaacatta
    87901 tgaagtagct agaggtgtac aatcaatact tcaaagatat aaagaacttc aagatataat
    87961 tgctatactt ggtatggatg aattatctga tgaagataag ttaatagttg cacgtgctag
    88021 aaagatacaa agattcttat ctcaatcatt cacagttgct gagcaattta caggaaatcc
    88081 aggtcaatat gtacctgtta aagagactgt aagaggattt aaagaaattc ttgaagggaa
    88141 acatgatgac ttaccagagt cagcattctt atttgtagga acaatagaag acgctgttag
    88201 aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt cattgaaagt tgtaactcca
    88261 agtaatactt tttatgatgg agatgttgaa atgatagtag ttagaactac aaaaggagac
    88321 aaaggggtgt tgaaaggtca tataccattt gtttcaggta tacctgtagg aactttaaag
    88381 ataaaaaaag agggaagttt taaagaggcg aaaatagcag aaggattcat taatgtaact
    88441 caagataaaa ctgtcatatt gacagaatct gctgagtgga tttaataaat taatttagag
    88501 tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa tggctttttt gcgttttggt
    88561 gttaataaaa taacgataaa attgtaagtt tttagtgaca ataaattcac aaaatgttta
    88621 aatttactgc caaattatgt tgtaattaat aagataaaga agatttagat agcttttatg
    88681 taagtgaaaa agtcttcata taagaaatac atataaaatt tttataaaat ttaaaaaatt
    88741 tgcttagttg tgtagtataa taaataatat aatagatttg tatataatat tcattacaaa
    88801 taattttaaa agttacagtt ttattataat gattattaaa ttaagataag taacataaac
    88861 taaataataa tgtgtattat cgatagtaaa tttataaatt cattattaat tattgaaaga
    88921 agtattataa attttctgga ggaacaatga atatatttga tattggcgta gatataattg
    88981 aaattgatag aataagaaag gctgtagaca aaaataatag gtttttggag aagatattta
    89041 cagatagaga aatagagtat tttaattcta agaattttaa agcagaaagt atagcaggta
    89101 atttcgcagc caaggaagct ataagtaaat ctataggtac aggaattaga gtgtttaatt
    89161 ttaaagatat agaagttctc agagatgaaa tgggaaaacc aatagtaaaa acttataata
    89221 atctagcaaa aatgtgcata gattataatg ttttagaaat aaaagtttca atatcacatt
    89281 caaaagatta tgctatagca aatgcgataa ccataattaa ggactaagga gtgattacat
    89341 tatgatggat aaaacagcaa aagaaataat gacaacagat gtaatagtag caaaacaaga
    89401 tgatagtata gcagatgtag ctaatatgct aattgcagaa aaaataggag gtctgccagt
    89461 tgtagattca gagaatagag ttgttggaat aatttctgaa acagatattc ttaaaaaaga
    89521 aaaatatata gaagcacctc tctatataaa tctattacaa ggattgattt tcttagatga
    89581 cttaaaaaaa gttgaaaaag atataaaaca agtagctgct tataaggttg gagaacttat
    89641 gtctaaagat ataataaagg ttcatgaaga tgataagttt gatgatgtag ctaatgtaat
    89701 gattaaaaaa tcaataaaca gagttcctgt agtagatgat gataacaaac ttaaaggtat
    89761 aatttgtaga tatgatataa taaaagcttt atacaatgaa taaactgaag taggaggtgt
    89821 gatgtgagaa aaaagtggac catagtatgt attatgttct tggcactggt tataattgta
    89881 ataggatgcc aaaaacgaca gtccacaaaa gaagaagtat acaaagattt tcaaaagcag
    89941 atttctgata tgaattatta cagttgtaaa gctgaagtag aagtagttgg aaataaaagt
    90001 ccacacaatt atgttttaat tcatacttat aaaaaaaccg ataactataa attagaagta
    90061 atttccccaa aacatttaaa agggaaaagt atagaatatc aaggtgataa aatattagtt
    90121 aaaaatccaa agattagtga tgtagtggag ttacctaata caggaaaaaa taatcaatat
    90181 ctttttgtag gagactttat aaaaaattat cttcaaaatg aggagatgaa agtaaagtta
    90241 tccaaaggac atttggtttt agagactttt attcctgggg ataataaata ttttaacaag
    90301 caagtattat atgtaaatgc tgacacaaaa aatcctgaaa aaatggaagt gttagataaa
    90361 gagggagtgc caagatttac agtaaaatac aaagattttg aatacagaaa ctaaggggga
    90421 ctaaagacat gcaaaaaata acagtgccta catgggcaga gataaatcta gataacttaa
    90481 gatttaactt aaataatatt aaaaatttat tagaagaaga tattaagatt tgtggagtaa
    90541 taaaagctga tgcatatgga catggtgcag tagaagttgc aaaattgcta gaaaaagaaa
    90601 aagtagatta cttagcagta gcaagaactg ctgaaggaat tgaacttaga caaaatggca
    90661 taacacttcc tattttgaac ttgggatata ctccagacga agcttttgaa gattctataa
    90721 aaaataaaat aactatgaca gtttattctt tagaaacagc acaaaagata aatgaaattg
    90781 caaaatcttt aggagaaaaa gcctgtgttc atgttaaaat agactcaggg atgactagaa
    90841 taggtttcca acctaatgag gagtcagtac aggaaataat agaattaaat aaattagaat
    90901 atatagattt agaaggtatg tttactcatt ttgctacagc tgatgaagta agtaaagagt
    90961 acacttataa acaagctaat aattataaat ttatgtctga taaattagat gaggctggtg
    91021 taaaaatagc tataaaacat gtatcaaaca gtgcagctat tatggattgc cctgatttaa
    91081 gattaaatat ggtaagagca ggaataatat tatatggtca ttatccatct gatgatgtat
    91141 ttaaagatag attagaatta agaccagcca tgaaattaaa atcaaaaatc ggacatataa
    91201 aacaagttga accaggtgta ggaataagtt atggactaaa atacacaact acaggcaaag
    91261 aaacaatagc tacagttcca ataggatacg cagatggatt tactagaatc caaaaaaatc
    91321 caaaggttct tattaaggga gaagtgtttg atgtagttgg tagaatatgt atggatcaaa
    91381 taatggttag aattgacaaa gatatagaca taaaagttgg agatgaggtt atactatttg
    91441 gagaaggcga agttacagct gagcgtatag ctaaagactt aggaactata aactatgaag
    91501 tgttatgtat gatatcaaga agagttgacc gtgtttatat ggaaaataat gagcttgtac
    91561 aaataaacag ttatttgcta aaataaaacc atgatattat aatctgggag gcgattttgt
    91621 gtacgataag agtaaagaaa aaatagaagt tactttacct gactctctga tttctgaagt
    91681 tgatagtata gttcagatgg agaacagtaa cagagaagat tttgcaaaag ctgcttttca
    91741 gttctatata actcagaaaa agaaaattca tattaaagaa tctatgaaaa cgggttatag
    91801 agaaatgggt caaattaatc tgtcactagc tgaattaggt atgacagttg aagaagtatc
    91861 ttctgatgat aaaaatgaag ggaagattgc ccaaggtgag tactaattgt gaaataaagc
    91921 gtggtgattt atattacgct gatttaagtc cagttgttgg ttcagagcag ggaggcgtta
    91981 gacctgttct gataatacaa aatgatgtag gtaataaata tagtcctaca gttatagtat
    92041 cagctataac atctcagatt aataaagcca aattacctac tcatgtagag ataagttcca
    92101 atgaatatgg gcttaataaa gactcagtaa ttcttttaga gcaaattaga actatcgata
    92161 aaaagagact gagggaaaaa attggttgtc tagatgaaaa tatgatgata aaagtagatg
    92221 atggacttca aataagttta ggattgttta ctttttaaag agggatttgt cttttattag
    92281 acaatccctt ttttctttta aatatctctt aaggtttaat tttaatgtac tatgttaata
    92341 aaattaaaaa taacgcaaaa aaatagcata aaagtgatat aataatatgg aattgatata
    92401 aaattacgaa ctcaagggag gtaattgcat gagagaccat aaaggattaa atgagattgc
    92461 gcgtatcata agaagagata tagtttcaat gatacataga gccaaatctg gacatccagg
    92521 agggtcatta tcagtagttg aaatacttac tgcgttatac tttgatgaaa tgaatgtgga
    92581 ttcttcaaat cctaaaatgg aggatagaga tagatttgta ttatcaaaag gacatgctgc
    92641 accagcatta tatgctacat tggctgaaaa aggttatttt gataaggaag aactgaatgg
    92701 attaaggaag ataggaagga tgttacaagg tcatccagac atgaaaggca cgccaggtgt
    92761 ggaaatatct actggttctt taggtcaagg tttttcagtt gcatgtggta tggctatggc
    92821 atcaaagttg gataatgctc cgtggaatgt gtatactttg cttggtgatg gtgaggttca
    92881 agaaggaatt gtttgggaag ctgctatgag tgctgcacat tataaacttg ataatttaat
    92941 tgctttttta gataataatg gacttcaaat agatggagat atagaaagcg taatgagtct
    93001 aggttcaata gttgataaat ttaaagcttt tggttggaat gtaattgaga tagatggaca
    93061 tgattttgac caaatatttg cggctcttga tatagctaaa tcaacagtta aaaaacctac
    93121 aatgatagtt gcaaaaacta taaaaggcaa aggaatatct tttatggaaa atcaagcagg
    93181 atggcatggg acagctccaa gtgatgagga gctagaaaaa gccttgcttg aattaggagg
    93241 tgctgacaat gagtaaaatg gcgacgagag aagcatatgg taaagcatta gtaaaattag
    93301 gtcaaataaa tgacaatgtt gtagtattag atgcagattt atcaaagtct acaaaaacac
    93361 atgattttta caagtcattt ccagatagat tttttaatat gggtatagct gaacaaaatt
    93421 taataggcgc tgcttgtgga ttatcaactg ctggtaaaat accttttgca agtacttttg
    93481 ctatgtttgc tacgggaagg gcttttgaga ttataagaaa ttcagtttgt tatccaaagc
    93541 taaatgtaaa aatatgtgct actcatgcag gtcttactgt tggagaggat ggagcatctc
    93601 atgaaagtgt ggaagatatt gctataatga gagctatacc aaatatgaca gttcttgttc
    93661 cagctgatgg agttgaaaca gaaaaaatta tttttgaaat agctaagtat aatggacctg
    93721 tttatgttag attagggaga agttcagtac cagttttatt tgatgaagat tataaatttg
    93781 aaataggaaa aggtacagtc ttaagagaag gaaaggatgt atcaattata gcttgtggaa
    93841 ttatggtaaa tgaagctcta ttagcacaag aaaagttgca agaagaaggt ataagtgcta
    93901 gagttataaa catgtcctcg ataaaaccta tagacaaaga tttaatatta gaatcagcaa
    93961 aagaaacgaa tgctattgta actgtagaag aacatagcat aattggggga ttaggttctg
    94021 ctgtaagtga ggtagtagga gagtcttgtc caaccattgt aaagaaagtt ggaattaaag
    94081 atacatttgg agaatctggt actccaaatg aattgttaaa aaaatatgaa ttaacttgtg
    94141 atgacataat aaagactgtt aaagaagcta ttattgcaaa aagaatgtaa gagatataaa
    94201 ttattcatgt ttgttaataa tataatatat ttaaaagaga ggtataaagt gttatattaa
    94261 tttatatctc tctttttttt atatgatatc aatatatcaa aaatgtaata aaatattcaa
    94321 atttttacaa aaatagagaa aatattcaaa tatatattga ctattcagaa aaatatgact
    94381 aatatataat ttatagagaa actaatttta tatataatga ctacttagat aaaagaatct
    94441 aaaaaaatta tgagaggagg agtagggtat aaaatcagat tataagtttt aagttattat
    94501 actaaaaagc tataaaacag ttatcatgta ttttatgtta aaggaaattg taggtagttt
    94561 atcgaattgt ttaatcagaa gaaaaaattt cacatgggga ggtaactgag atatgtcaga
    94621 aaaagtaaat gttaaaacag tcatcagttt cgcaggtgcg tatgtagcaa cagtaatcgg
    94681 ttctggattt gcaacaggac aggaaataat gcaattcttc tctttttatg gttttgcagg
    94741 tattattggt gggataattt caatggtatt attctcatgg atgggtgctt cagttatgtc
    94801 aaaaggtaaa gagcttcaac ttaaagaacc tatcaaaatt tatcgtgttt attgtggtaa
    94861 gtatttagga atattctttg aatggtttgg acctttattc ttatttggtg ttttcgtagt
    94921 aatgatttca ggagcaggag caactttaac tgaatactat ggattaaatc catttgtagg
    94981 tagagtaggt atggcagtag tagcattatt aactgtttca ctaggtcttg ataaattatc
    95041 aaagatacta ggtggaatag ggcctataat aataatcttt acacttttag ttggtggtat
    95101 aagtttagct aaaaacatag gtaatatagg agaagctgca caagttctta gcactgtaaa
    95161 gatagctaaa ccagttccaa atgcctatct atctggtgta atatatacta cttataacac
    95221 aattgttgtt atggcattct taactggatt aggagcaagt gctgctaaca aaaaagaagc
    95281 tatttgtggt ggtatattag gtggagtagc acttatggct gcagctataa tgatgcactt
    95341 agctattctt tctgatatag gaaacttata ttcaaaagca ataccatcat tattcttagc
    95401 agataagatt tcaccagtaa ttggagtttt attctcaata attcttatat taggtatata
    95461 tacaacagct gtacctttat tatggtcagt aactaataga tttgtagatg ataaacatcc
    95521 taagtttaaa ttaataactt taatagcagc tatattaggt cttataggtg gcttcctacc
    95581 atttgataag ctggttggta ttttatatcc atatactgga tatatgggcg tcataatact
    95641 ggtttgtgtt ctttatagac agattacaaa aacatctgga tatcaagaag gaatcagtga
    95701 caaataagta ttttttaata ttgtaaactc aagagctata tgaaaacaat gaaaatgttt
    95761 tcatatagct ctttttattt tttagctcgt ttattatgaa atagaaaaaa catgtttttt
    95821 agttacatta catatattta taattatgta taaatatata ctaatcaata atttagagtc
    95881 taagataagc ttataaatca tatatttaga atatatggga ggagtgataa gattgaatgt
    95941 aaagtttaat attaaaggta taatttatgg agttatagct cttgtattcg ttataggggg
    96001 atttatattc ataccaagta tgtttgtaga gaaatctaaa cccatagatt atacagtatt
    96061 acaaagaaat tcaataccag aaaagatatt agatgtgatg gataagtata caaatgaaga
    96121 aagagcatta gcggctaaaa tcgataacaa aatatacatt atagtgacta gaggaaataa
    96181 taaatatggt atagaaatgg acaaaataga aagtgtggtt gaggaaggaa aggatgtttt
    96241 gagagtcaag ataaagtata aagataagga agattctttt ccatacatag ttgttgaaac
    96301 aaatatgagt gaacttccag atagaataga attaaatgcg acgaaataag aaggtatttt
    96361 ataattaaat aactgcatat tgtacaataa agtcatggtt tttattaatg taagtaaatt
    96421 gtatatggtt cttattgttg taaaagccat gacttaaaat gcttataaag taagatatta
    96481 acagtagtat ttattactac attaaatggg gtctggaaat acttaatatt ctaattattt
    96541 ggagtgataa ttctagtttc agtaactatg tagtctaatt gtgcatcatg agtttctttt
    96601 ggaatagaat cgaatatctg gaagtcaaat gctatgccga tagttatagc atcttttctt
    96661 aagttttcta aaaatctatc ataaaatcct ccaccatatc ctaatctata gcaatgtata
    96721 tcatatgcta cagctggtac aattacaaca tctaaggcct taatatcaat tgtaggtgat
    96781 ttttcattag gttctcttat tccatatgct ccaattctca atccctcttt taaatctgtt
    96841 atttgagtag ggattaatgt atgattttct tttattgtga tgggacttga tactatttta
    96901 tttagagaca aaagtttttt gattaaatta tctgtttgaa cttcattgtt aaaatttagg
    96961 taaagcatta tattagttgc atttcttata tcttccattt gaagtaattt ttcagttatt
    97021 aaatttgaat tactaagtac aaagtttgca ctcttatttt ttcttgattc aataactttt
    97081 tttctaaatt cttttttcat aaaataattc ctcccttaat atgttataat gtgtacagca
    97141 attgtattta atataaattt tagtataatt tattgtaata aagcaatata tttgggtaaa
    97201 tgtaataaaa ataacagcta gtcatataat aataatagtt tatgtaaaag gagatgtatc
    97261 ttgtgatttc aaagaagaaa gctatttttt taggggtgat tttagtaatt ataacagcta
    97321 tggttacatc agcatttcag ttgacattgg ggaataaagt tgtaatatcg aaagaactct
    97381 atgaagacta taaaaaatat gataagttgt taggactaga atctattata aaacaagatt
    97441 tttataaaaa agtatcagat actgatttgg taaatggagc agcaaaaggt ctatttctag
    97501 gaacaaatga taagtattct ggttattata ctaaagatga aatggagaat ctaattaatg
    97561 atagtgaagg ttcttatgtt ggtgtaggta tgtatatagg ggcatcaaaa gatggtggct
    97621 tagtagtagt tcccatgaaa gattctccag cagagaaagc aggtgtaaaa tcaggggata
    97681 aattagtaaa agtcaatgga aaatctgtct catataaaaa ttcagatgaa gctgttcgta
    97741 tgatgaaggg taaaaaaggt aaaacagttg aactgactat attaagagaa gataaacaat
    97801 tgaactttaa agtaaaaaca gaccagataa ttgagaagag tatagaaagt aaagtaattg
    97861 ataatgattt aggatatata gaaattaccc aatttatatc aagtacatat actgactttg
    97921 ataaagcttt aaaagaatta aaagcaaaaa atatcaaggg attagtaata gaccttagaa
    97981 ataatcctgg tggaatgcta gatatatgta aagaagttgc tgatgagtta attggtgaag
    98041 gaaccatagt atatacaaaa gataataagg gaaatactga gtacttgaaa tctgataaag
    98101 aaaaattggg acttccaata gtagtgttaa ctaatggaga aagtgcttct gctgctgaaa
    98161 tattaacagc tgcaatcgtt gataataaag aaggtatttc tgtagggact acaacttttg
    98221 gtaaagggtt ggtacaatca gttgtaagat tgaaggatgg aacaggatac aagttaacta
    98281 ctgctcaata ttttacacct aatggagatt atatcaatga aaaaggtata aagcctacta
    98341 ttgaagaaaa agatgaaaat aaacagttag atgtagcaac taagtggctt agagaacaga
    98401 ttgataaata ggatttaatt tttataaact tatactataa aataaagaac attataagag
    98461 agatgtctca aaatgaactt tttagttcat gagggagtct ggaaatattt atgtgtattc
    98521 tttaaataaa aatttatttt ttaagtgata attaaatttt tacagatagg gctgtggttt
    98581 taaaaatcat agccttattt tgatattttt taaaaacata ttgcaaccca gttgttccaa
    98641 atattatatc atttactttt aaaatatcgc aaactgcata ttatatattt attataatat
    98701 aatttatcta actagaacct cagtttacta attaaattag atgcatagaa taactttttt
    98761 aaacattgaa aataaattaa actctaaaca catttaaata taaatttata tatcattttg
    98821 acttggttaa acaatttcat acttatcgat ataaaaatag gttataata
//
LOCUS       DAMJYC010000002        94047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJYC010000002 DAMJYC010000000
VERSION     DAMJYC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 94047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.002"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            546..1577
                     /locus_tag="PC472_000101"
     CDS             546..1577
                     /locus_tag="PC472_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2725479.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1710..3008
                     /locus_tag="PC472_000102"
     CDS             1710..3008
                     /locus_tag="PC472_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2725480.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3122..3367)
                     /locus_tag="PC472_000103"
     CDS             complement(3122..3367)
                     /locus_tag="PC472_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2725481.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3515..4381
                     /locus_tag="PC472_000104"
     CDS             3515..4381
                     /locus_tag="PC472_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2725482.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4385..4921)
                     /locus_tag="PC472_000105"
     CDS             complement(4385..4921)
                     /locus_tag="PC472_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2725483.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5112..5315)
                     /locus_tag="PC472_000106"
     CDS             complement(5112..5315)
                     /locus_tag="PC472_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2725484.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5606..6370
                     /locus_tag="PC472_000107"
     CDS             5606..6370
                     /locus_tag="PC472_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2725485.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6759..8072
                     /gene="prdC"
                     /locus_tag="PC472_000108"
     CDS             6759..8072
                     /gene="prdC"
                     /locus_tag="PC472_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2725486.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8476..8561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9143..11290
                     /locus_tag="PC472_000109"
     CDS             9143..11290
                     /locus_tag="PC472_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2725487.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDSQTPDVEKPSDGQMPDVEKPLDEQTPEEEKPLEEK
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSKDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGGEENELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCDKLIERAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPP
                     VPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPSVSDTVEKTPELSRDDTIIK
                     SPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            11697..13457
                     /gene="prdR"
                     /locus_tag="PC472_000110"
     CDS             11697..13457
                     /gene="prdR"
                     /locus_tag="PC472_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2725488.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13886..15766
                     /gene="prdA"
                     /locus_tag="PC472_000111"
     CDS             13886..15766
                     /gene="prdA"
                     /locus_tag="PC472_000111"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2725489.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15847..16131
                     /locus_tag="PC472_000112"
     CDS             15847..16131
                     /locus_tag="PC472_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725490.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16169..16894
                     /gene="prdB"
                     /locus_tag="PC472_000113"
     CDS             16169..16894
                     /gene="prdB"
                     /locus_tag="PC472_000113"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16619..16621,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2725491.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16974..17732
                     /gene="prdD"
                     /locus_tag="PC472_000114"
     CDS             16974..17732
                     /gene="prdD"
                     /locus_tag="PC472_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2725492.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17747..18214
                     /locus_tag="PC472_000115"
     CDS             17747..18214
                     /locus_tag="PC472_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2725493.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18232..18714
                     /locus_tag="PC472_000116"
     CDS             18232..18714
                     /locus_tag="PC472_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2725494.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18734..19741
                     /locus_tag="PC472_000117"
     CDS             18734..19741
                     /locus_tag="PC472_000117"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2725495.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19861..20742
                     /locus_tag="PC472_000118"
     CDS             19861..20742
                     /locus_tag="PC472_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2725496.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            21410..22045
                     /locus_tag="PC472_000119"
     CDS             21410..22045
                     /locus_tag="PC472_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2725497.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            22229..22798
                     /locus_tag="PC472_000120"
     CDS             22229..22798
                     /locus_tag="PC472_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2725498.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            23010..24785
                     /locus_tag="PC472_000121"
     CDS             23010..24785
                     /locus_tag="PC472_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2725499.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKVDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            25209..26516
                     /locus_tag="PC472_000122"
     CDS             25209..26516
                     /locus_tag="PC472_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2725500.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            26844..27383
                     /gene="hpt"
                     /locus_tag="PC472_000123"
     CDS             26844..27383
                     /gene="hpt"
                     /locus_tag="PC472_000123"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2725501.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            27741..29417
                     /gene="bclA2"
                     /locus_tag="PC472_000124"
     CDS             27741..29417
                     /gene="bclA2"
                     /locus_tag="PC472_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2725502.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGVNGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(29924..30679)
                     /locus_tag="PC472_000125"
     CDS             complement(29924..30679)
                     /locus_tag="PC472_000125"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2725503.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            31119..32108
                     /locus_tag="PC472_000126"
     CDS             31119..32108
                     /locus_tag="PC472_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2725504.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLRMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(32560..33276)
                     /gene="dapD"
                     /locus_tag="PC472_000127"
     CDS             complement(32560..33276)
                     /gene="dapD"
                     /locus_tag="PC472_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2725505.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYDDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(33500..34249)
                     /locus_tag="PC472_000128"
     CDS             complement(33500..34249)
                     /locus_tag="PC472_000128"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2725506.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(34316..35203)
                     /locus_tag="PC472_000129"
     CDS             complement(34316..35203)
                     /locus_tag="PC472_000129"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2725507.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(35320..36321)
                     /locus_tag="PC472_000130"
     CDS             complement(35320..36321)
                     /locus_tag="PC472_000130"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2725508.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(36746..37627)
                     /locus_tag="PC472_000131"
     CDS             complement(36746..37627)
                     /locus_tag="PC472_000131"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2725509.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            37950..39143
                     /locus_tag="PC472_000132"
     CDS             37950..39143
                     /locus_tag="PC472_000132"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2725510.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            39349..40467
                     /locus_tag="PC472_000133"
     CDS             39349..40467
                     /locus_tag="PC472_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2725511.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYLKLLKYKEAID"
     gene            40598..40843
                     /locus_tag="PC472_000134"
     CDS             40598..40843
                     /locus_tag="PC472_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2725512.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            40830..41576
                     /locus_tag="PC472_000135"
     CDS             40830..41576
                     /locus_tag="PC472_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2725513.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLAKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            41859..42734
                     /gene="hslO"
                     /locus_tag="PC472_000136"
     CDS             41859..42734
                     /gene="hslO"
                     /locus_tag="PC472_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2725514.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            43125..43925
                     /locus_tag="PC472_000137"
     CDS             43125..43925
                     /locus_tag="PC472_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2725515.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGIHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            44452..46692
                     /locus_tag="PC472_000138"
     CDS             44452..46692
                     /locus_tag="PC472_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2725516.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWNWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEAEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            47106..48641
                     /locus_tag="PC472_000139"
     CDS             47106..48641
                     /locus_tag="PC472_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2725517.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            48657..49418
                     /locus_tag="PC472_000140"
     CDS             48657..49418
                     /locus_tag="PC472_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725518.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            49756..50625
                     /locus_tag="PC472_000141"
     CDS             49756..50625
                     /locus_tag="PC472_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2725519.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            50954..51205
                     /locus_tag="PC472_000142"
     CDS             50954..51205
                     /locus_tag="PC472_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2725520.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            51317..52096
                     /locus_tag="PC472_000143"
     CDS             51317..52096
                     /locus_tag="PC472_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2725521.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(52231..52881)
                     /locus_tag="PC472_000144"
     CDS             complement(52231..52881)
                     /locus_tag="PC472_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725522.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(52878..53381)
                     /locus_tag="PC472_000145"
     CDS             complement(52878..53381)
                     /locus_tag="PC472_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2725523.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(53430..53945)
                     /locus_tag="PC472_000146"
     CDS             complement(53430..53945)
                     /locus_tag="PC472_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2725524.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            54225..54752
                     /locus_tag="PC472_000147"
     CDS             54225..54752
                     /locus_tag="PC472_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2725525.1"
                     /translation="MHEQIDCCERKKIKTINIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            54736..56139
                     /locus_tag="PC472_000148"
     CDS             54736..56139
                     /locus_tag="PC472_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2725526.1"
                     /translation="MEEKYKETLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(56658..56969)
                     /locus_tag="PC472_000149"
     CDS             complement(56658..56969)
                     /locus_tag="PC472_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725527.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(57025..57618)
                     /locus_tag="PC472_000150"
     CDS             complement(57025..57618)
                     /locus_tag="PC472_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2725528.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            58469..59119
                     /locus_tag="PC472_000151"
     CDS             58469..59119
                     /locus_tag="PC472_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2725529.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLAQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            59333..60004
                     /locus_tag="PC472_000152"
     CDS             59333..60004
                     /locus_tag="PC472_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2725530.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKA"
     gene            60001..61131
                     /locus_tag="PC472_000153"
     CDS             60001..61131
                     /locus_tag="PC472_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2725531.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            61563..61673
                     /locus_tag="PC472_000154"
                     /pseudo
     CDS             61563..61673
                     /locus_tag="PC472_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            61999..64086
                     /locus_tag="PC472_000155"
     CDS             61999..64086
                     /locus_tag="PC472_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2725532.1"
                     /translation="MQLTKIILSNYRCFGKEPTTIEINNLTGFIGHNSSGKTALLSAL
                     IKLFGEKSSDRIIERSDFHIPESTDPDSVDMNELYIETQFEFPELERDEDNLSIPVFF
                     KNMVVNEPGGKPYLRIRLEASWEKSSNPEGSIDTNIYYITSTTEDINPEDKRNIKRHD
                     LDQIKIIYVPAIRKPDEQLRNVSGTIISRLLNGISWSEQTKTKIKTKANETEKIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTETHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDDKEEEIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNLISKKENSQTILTSHSPAIVKRIEPTDIRYFRICKEHECTKVKSII
                     LPNKEKEVEQFKFVKEAVLAYPELYFAKLVVLGEGDSEEIIIKKMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLIKIGRDKNV
                     LLDTKDGILQDNEFKDMHTWNVTKTEIMLEWIKMLKEYDVYFSSPLDIDFAMLQSFKD
                     KYIATLESSQGPIIKGYGKIQNLEINEDTEEGLKNAYQARIVSDIKSTLKQEGGDGST
                     YTEEEKKLMIWYSYFFLGRGKPTTHLSLFSNNTDLDFESFPECLKEFVNRVIEKMIDD
                     LGDEEDNEEELDC"
     gene            64064..65935
                     /locus_tag="PC472_000156"
     CDS             64064..65935
                     /locus_tag="PC472_000156"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2725533.1"
                     /translation="MKRNWIVKEEWHPASGFKLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCKYPKKILAISFKKDAADNLKERVELRCGKELALRFESKTYDA
                     FAKEIVDRFRNSLDEKYRPLNNYNIATNGDVRRAFEISGLNFRGNQTEFNKLYGNKLS
                     INKLPLHTNFVDKIFIDAWNVLLKGKEDNNFESSLTFEMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTTSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNRFNEDAIDLKTNEKWNHEDGEAYLRLFE
                     DYIKEADILSEEIVGLVESGIKINEICILVKQSVDIYSKEIIHKLSECNIKARNEAVY
                     QELLKEDIIKILVSLFRLSLNDRSPDDWDYIYDILGELYGIDEGYNPKVLNDFITKVE
                     LMINDIKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQQYKSKDYFDDLVDKFKKLV
                     WSEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQTHDCINEFYELFDDTKIVKRIEYCD"
     gene            66055..69891
                     /locus_tag="PC472_000157"
     CDS             66055..69891
                     /locus_tag="PC472_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2725534.1"
                     /translation="MKKKLKASKRILSFIMVFITIITTILTSSVSVYASELDMSEHTG
                     FNYLGISPITGRKINHNIYRMKMDNKVVFCVEPGIKTASGGGYISEEYINSKKDILSK
                     IAYYGYTDTNKSKYDYALTQIIIWEELGDKFISTTLPNYYKRKAEILEKVNKHNILPS
                     WNNEKVAVKSGETIELKDKNGIIREMKLESNNTGTNIVLEENTLKITANEKSTSGVIS
                     YRKIPENEVGTSIVYRKPNYQSLAEFHMASSMSAILNINVIKLGNIKAKKVDEDTGKA
                     LANTKLKFEYDGKGKEIITDSNGIANIEDIPEGTTVTISEVIAPNGYFNKGEIKKVVI
                     EANKTIEVTLGNKEQLGKVIISKSGKDFGIEMPNDSYKLEGAIYGIYNENDQKITTIT
                     TDKFGKATSENIKLGKYYALEEKAPEGYLISKEKIPFELKYAGQTVEVTSTSISQKDT
                     EQKGKAILIKEDEKTGSIPQGRAKLDGAVYELRKSNNDELVETVTIKGGKAKVENLYL
                     GKYYWIEKKAPEGYLLDTTKHYFEISYAGENVETAIKETLVKEKVITGGFDLIKFGEY
                     DWKEKIKNIFKTKNKEIKSLKDVEFSVFSDTTGKLVKTGYTDEKGYLKFERLPYDTYT
                     VKETKTPEGYIAAKDFKVTVKKQNETHHYAIQNRVIEEKLKVVKIDSVTKKIIPVKGA
                     EFKIKSLQTGDFITMPKNNEDEKTDMFYTNDEGYLITPEALSYGEYELTEVKAPDGYV
                     LAKDPIHFKVDGSSNGIIEIRFENTSQKGIVEFTKKGQTPKDIKIEEMKYGKLHEIIY
                     EYKPLENVAYDIIAKENITTNDGTIHFKKGDVVETLTTDEKGKWKSSELYLGKYQAIE
                     KSAPNGYIISSNPIEFELSYSGQLVELTKTSLTANNDLQSLLVNIFKNEEKIEGWKDN
                     KPVIKDIKGNGKIFGIFTREKQVISDNIKIPKDSMVGIKEVKDGVAELNIKLPEGKYY
                     LKEIDSGENHILNEKEYDFEFNSTNNEKEIKVNIYEDGVDFGDKNENIKPILNKLQLN
                     SFKIKKINEKAIKNKRNGFSFEFTELGQGSKFILEDKKGDIIQELTIGKGSIGEFNNI
                     PVGTFYLKEKETSNDKYILSNKVIRIESTKEGIKAFDEDNNLISEKNNNEDKILLEIK
                     NELIKGGAKLIKTDLVTGEALPNTGIRILNENKNTLIEGKTDDKGVFYFDNLPKGIYY
                     FQEYEAPKGYQIDETPMKFEIKENGEVVTCKMTNKKIEKVSIPKTGDYNSIILYGIVL
                     LASGTLLLKMRRKKDK"
     gene            70049..70363
                     /locus_tag="PC472_000158"
     CDS             70049..70363
                     /locus_tag="PC472_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725535.1"
                     /translation="MKLKHIIPNMEKTFGNLEYAGEGDVEQKRINGKMTVISRSYNLY
                     SNIQRADDIVVVLPANAGEKCFEVEERVKLINPKIIAEGYKIGTRGFTNYILLADDIV
                     KA"
     gene            70377..70718
                     /locus_tag="PC472_000159"
     CDS             70377..70718
                     /locus_tag="PC472_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725536.1"
                     /translation="MRLANGIVIDKEKTFGMLKFSALRREVRVQNDDGTPSNEIKERT
                     YDLKSRGQGRMIQVSIPASIPLKEFDYNAEVDIINPIADTVATANFNGTDVDWYIKAD
                     DIVLKNKKTSK"
     gene            70837..71856
                     /locus_tag="PC472_000160"
     CDS             70837..71856
                     /locus_tag="PC472_000160"
                     /inference="COORDINATES: protein motif:HMM:NF025637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725537.1"
                     /translation="MNQFKYEELESRYIYYMKNYNPIFNYVHDMGETQVIIDPIDKVC
                     RFCGKKYPEVKFKKKAHAISEMLGNKEFVLRNECDSCNIFFGQKLEDNLGKYLGLGRT
                     LSQIYGKEGVPSYKSRDRKSRVDFTDKGIVIQATIDSNFIEQYDNYIVFHSIRDTYTP
                     IAVYKALVKMALSLIPFEQMSLFKDTVEWLKEENHVISKHNMDNYTYVIERYVPGPKP
                     MQLRAMGFVRKYDVLLMPYYQFILEFANYSYQIIVPCKKKDKELCGHEIDFIPIPGTH
                     EVMCTATDRVLTELKYMGNKEKVKDEPLDICMNFEKCELYEGNGKSINEILKEEGIDL
                     SKRLK"
     gene            71908..73086
                     /locus_tag="PC472_000161"
     CDS             71908..73086
                     /locus_tag="PC472_000161"
                     /inference="COORDINATES: protein motif:HMM:NF027613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4935 domain-containing protein"
                     /protein_id="HDF2725538.1"
                     /translation="MLKYPLSVVLDTNIFIGCRYDLNEEGLLIKLKNLVNNNKVRIYI
                     SNIVLRETERHIKADISKAIQELKQARRNISKNISPTIVNNTSLENIFGLPCQNTIEK
                     TAITRFREFLDESKVIYLDNEGIDIDEILEDYFKGNAPFENNEAKKYEFPDAFIISKL
                     KKEFHKNRPVWVVSADRGFRKALDSRDGFNCLASINELLDMINKQDQKMYDTIVEYIE
                     NKDAYKEICNKIEEKIESDDIEVNGLDYDRKGCCGGYEYIDTYITDVSVEKFYLSSVD
                     EISNDIVNLTIFCKAKIDVSCFYNDYDNSIWDSEEKEYMFLLNEEVDEEHEPEFECRL
                     SLKVNHYGDKVEFSLSDISYDLVLDQSSRTKQSLVEPEDPRIAAEADMMDALEEYYNH
                     "
     gene            73180..74583
                     /locus_tag="PC472_000162"
     CDS             73180..74583
                     /locus_tag="PC472_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2725539.1"
                     /translation="MKFYRCRGKRIRASDKSLFFRFLTSILSAIFIISLILFNGKQIV
                     QINWKNISLVLENGLLFLSIFCNILTIVLSISICIVTTFLYYYFKRDRITQLIHRQKL
                     ARMILENKWYESESIKDDGFFKDLNSNKQKEKIVYFPKMYYELKEGLIYVKVEITLGK
                     YQDKLLNLEKKLESGLYCELVSKELKDSYIEYVLLYDTIANRISIDEIKVRNGSLKLM
                     KSVYWEFDKLPHMLIAGGTGGGKTYFILTIIEALLSTDSILYVLDPKNADLADLRTVM
                     PNVYYKKDDMISCINNFYDEMMKRSETMKAMSNYKTGENYAYLGLPANFLIFDEYVAF
                     MEMLGTKENTSVLNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGR
                     MSELGYNMMFGESNKEFFLKQIKGRGYVDVGTNVISEFYTPLVPKGHDFLKEIDKIIK
                     SRQVEQAACEAKADGSN"
     gene            74775..75962
                     /locus_tag="PC472_000163"
     CDS             74775..75962
                     /locus_tag="PC472_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2725540.1"
                     /translation="MSIEAWIKNLRNKRIEYGISQNKLALSVGITREYLNKIESGKII
                     IKNDMKEKLIKSIERFNPDLPLTMMIDYIRIRFPTTDVKNVVNDILKLKLEYMIHEDY
                     GFYSYSEHYVIGNIFVLVSQDIEKGVLIELKGQGCRQFENFLLAQHRSWYDFLLEALL
                     AGGVMKRMDLAINDMTGILDIKELTNKCNNEECISVFRSFKSYRSGELVRRDEKIGMG
                     NTLYIGSLKSEVYFCIYEKDYEQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLA
                     NRDAERTAFSIINRYIRFVDKQKDKSRSEWKINEHWNYFIGEGREKLKLTTSPEPYNI
                     HKTLNWLSRQVAPTLKTIKRLDILNNTTLIEDLIKYANLTDRHKKIIEQNSVNIEDII
                     YDE"
     gene            76031..76252
                     /locus_tag="PC472_000164"
     CDS             76031..76252
                     /locus_tag="PC472_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725541.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLMAIVVIGVYLGFKREFSKLIGFLV
                     VALIAVGLVFNAAGVKDILLKLFNQIIGV"
     gene            76317..76910
                     /locus_tag="PC472_000165"
     CDS             76317..76910
                     /locus_tag="PC472_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725542.1"
                     /translation="MRESVKNFLVKKYNELDYKSSCTCLKYHYKYNGVNVNIYFDAYD
                     LNSLSLYLVLIYEQKYYYTPLNISNTNIHNEYLKEIPQEILNQILENNHLVEFYSYME
                     EYIKNNNPIPNSYKKDKIFINTLKYSKDKTDLPFWYHIRRVRMSDETLKKLSAKADIS
                     IKTLKSIQNKNLTLVRTADVAKRKELTFILGGEGITI"
     gene            76999..77502
                     /locus_tag="PC472_000166"
     CDS             76999..77502
                     /locus_tag="PC472_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2725543.1"
                     /translation="MEEMRVYIANLGKYNEGELVGDWFTPPIDWDDLKERIGLNSEYE
                     EYAIHDWELPFEISEYTSIEEINRLYSMAEELASTDMGEAISDIQKAFFSSFEELYEH
                     KDDIVYYPGCSDMSDVAYYLIDDCNMLGEISENVRNYINYEAYARDLEINYSFVVTNH
                     GVFEYIN"
     gene            77819..78706
                     /locus_tag="PC472_000167"
     CDS             77819..78706
                     /locus_tag="PC472_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725544.1"
                     /translation="MKKNENVEITKQAYSILENGYALQKSSCCLQGSFFEGVSDEDYK
                     KIDYKLKAIRQDFAKILVIMNSIDRTYSLYQKDEYVPSYISIMGNQATNEIGCFIEYL
                     YAKYRVILEYFQQILEICIPPRLSDPQSEQYNKLKKSHKKYNFLLKYLAENIDETNNI
                     LNMEWFQHIRVERDFIIHDGATCLVFGDKENLLFKVMTTDALDREYDNKQDEFFSNEK
                     GLIYYNRYWGVQISKLIVFSETIFRFLISISNMSEENKFILEDLFPQERNKFIDSYGT
                     EYNDVQDVLINMLRKIIEI"
     gene            78887..79291
                     /locus_tag="PC472_000168"
     CDS             78887..79291
                     /locus_tag="PC472_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725545.1"
                     /translation="MKKIKSYTGIWNVEKVIYAINDFQLPFPVTFTQMTWFVLTLFII
                     ILFGKLPPLYMIEGVFLKYLGIPVFITWFMSQKTFDGKKPYGFLKSIILYSLRSKITY
                     SGKSIKFKNKIIDENITIVRSELYVPDKIYRK"
     gene            79263..81710
                     /locus_tag="PC472_000169"
     CDS             79263..81710
                     /locus_tag="PC472_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725546.1"
                     /translation="MFPIKYIENNLVFNKEGECFAYYELIPYNYSFLSSEQKFIVHDN
                     FRQLIAQNRNGKIHALQIATESSIRSTQERSKKNITGRLKEIAYEKIDEQTEALVSMI
                     GDNQIDYRFFIGFKLLVTEEEMNIKNIKKSILANLSDFIYDVNNKLMGDFISINNDEI
                     MRFSKMEKLLENKISRRFKVRRLDKNDFGYLIEHNYGKKGTVFEDYKYHLPTKKLKRE
                     TLVKKYDLIKPTRCLIEEKQKYLKIIHEDSESFVAYFTIESIIGEMDFPSSEIFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALTTVRNKKKELKDLDNHAWQAGNETGTNIVEALDAVN
                     ELENNLDQTKESMYKLSYVIRVSADSIDELKRRCDEVKDFYDDLNVKVVRPMGDMIGF
                     HNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQQLGENEGIYIGYSLDTGRNVYLKPS
                     LASQGVKGSVTNALAAAFLGSLGGGKSFANNLIVYYAVLFGGQAVLVDPKAERGNWKE
                     DLPEIAHEINIVNLTSDIENKGMLDPYVIMKEVKDSESLAIDILTFLTGISSRDGEKF
                     PILRKAIRNVTQSKCRGLLKVIDELRKDNSQISNSIADHIESFTDYDFAQLLFSDGNI
                     KGSINLEKQMNIIQVEDLVLPDKDTSFEEYTTMESLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWTFLNVAQGKTLSNKLVRAGRAMNAGVYFVTQNAGDVSDENLKNNIGLK
                     FAFRSTDINEIKRTLEFFGVDKEDDNNQKRLRELENGQCLIQDLYGRVGVIQIHPVFQ
                     ELLTAFDTRPPIRKEVD"
     gene            81710..83926
                     /locus_tag="PC472_000170"
     CDS             81710..83926
                     /locus_tag="PC472_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2725547.1"
                     /translation="MKSKVFRNISWMIILIVILVLFFIYISESIVYAEGLVDETVNSN
                     NVYSMYPLRNYQLDFYVDNSWGWLPWNWTDGIGKSVQYGLYCITNFIWIISLYLSNAT
                     GYVVQEAYKLDFINDMADSIGKSIQTIAGINQKGISNNGFYIGFLLLFILILGIYVAY
                     IGLIKKETSKAINSVINFVVIFIISASFIAYAPEYIKKVNDFSSDISTAALNLGTKIV
                     TSNSNSEGKDSINLIRDNLFSIQVQQPWMLLQYGNSNIEEIGKERVEKLISTDPGEDD
                     GKKREEIVKTEIEEKHNNNLTITEVMSRLGKVFFLIIFNIGITIFIFLLVSMMIFSQI
                     LFIIFSIFLPLSFLLSMIPSYESIGRQAVVKLFNTIMMRAGITLIITVAFSISTMFYT
                     ISKDYPFFMISFLQIVTFAGIYMKLGDIMGMFSLHSRDSQNMSSRIFRKPYRKMQNKS
                     KHIERYIRQALISRGKDKNKLSFNRSNKGNINNLQKRVDYLPSRDDYNKSINKESFLK
                     HMGNNIGAVIDTKKNIADKSRQVKENIKDIPIQTKYAFHNTKEKTKDNISDFKRGIVE
                     EKEKRQQGRDKKQQNHRETIAQKRAKLGNVVVSKDKNSKDNKNIDNYSNLKDKPSIGS
                     ENVKKKKGENIRPTVSEKIVNDKNLEKSNSINKIEMPTEEILHKKRDITNQGKESSNK
                     VKNNQNEKFKNSKAIKIERHNINSNSKDSRYNHINSKERKIKNKPNFTPENKVRKQ"
     gene            83923..84924
                     /locus_tag="PC472_000171"
     CDS             83923..84924
                     /locus_tag="PC472_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2725548.1"
                     /translation="MKIKISIIFISIFLCIFGVILLFIPLISDENFNQDRNQNIIGMN
                     LSPEVLKYKPIVERYAQKYDIKEYINYLLAIMQVESGGILVDVMQSSESLGLSPNTLG
                     TEESIEQGCRYFSNLLKTSKEKGCDLNSTIQAYNYGFGYLNYIQSNGKVHNYNLAERF
                     SQNMAQGVKVTYTNPIAIAKNGGWRYKYGNQFYVELVLQYIGTSEFSNETVQIIINEA
                     LKYKGWKYVFGGDNPNTSFDCSGLTQWCFKKAGINLPRTAQEQYDIMQHITLDQAKPG
                     DLVFFHGTYNAGTYVTHVGIYVGNNKMYHAGDPIGYADLTGSYWQKHLIGSGRIKNR"
     gene            84934..85827
                     /locus_tag="PC472_000172"
     CDS             84934..85827
                     /locus_tag="PC472_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725549.1"
                     /translation="MLRKKDKKHKVNKISVIRLGINRKSVVVLWIVLIVSIVFGVYKN
                     FTAIDKHTIHEKEIIEQKIIDTNGIENFVKNFAKEYYSWENNKESIERRTSKINEYLI
                     KELQELNRDTIRNDIPTSSSVKDIKIWSVNQNKENELDVKYSVEQTIKEGEDVKDVIS
                     SYEVVVRRDNSGNMIIIKNPTLSQMPEKLNYEPKKEETDSTIDQKTIKEITEFLNTFF
                     KLYPNASKNELEYYVKEDVLKPINKEYIFSELVNPIFTREEKNIKVSTSVKYIDQQTK
                     AIQISQYNLTLEKDGNWIIRK"
     gene            complement(86147..86548)
                     /locus_tag="PC472_000173"
     CDS             complement(86147..86548)
                     /locus_tag="PC472_000173"
                     /inference="COORDINATES: protein motif:HMM:NF013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2725550.1"
                     /translation="MENIMSFLGDANTPIAVSNIHNGKPQVRFISFKMIEGGKLYFLT
                     SKKKDFFNQLVENNNIQICSMPNENNELLRITAKVDFIEDLDLNKKAFEILPLLQKVY
                     ETPANKDIILLSISYQNIVKYSLSEILYCWL"
     gene            87081..87902
                     /locus_tag="PC472_000174"
     CDS             87081..87902
                     /locus_tag="PC472_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2725551.1"
                     /translation="MKKYIPSVILLCIFEIVAVTLWITMDNLFYMINFTYIGVCISIG
                     LALFANEKKYARRFVQFAVGSYMLIYLGVIQQENMQIEGFWYYLFLGVFEAATIHYTV
                     AKIFGPLIFGRGWCGYACWTVMILDLLPYKQPKSPRKNWGFVRYITFTLSLVLTIGFF
                     LIKIDNLDKIIFLLFIVGNVLYYIVGISFAFVLKDNRAFCKYICPVTVFLKPMSYYSI
                     IRVHCDEDKCIKCGKCLKVCPMNVEVNKDSRKRTNATECILCYECVKECPVNALR"
     gene            88224..92333
                     /locus_tag="PC472_000175"
     CDS             88224..92333
                     /locus_tag="PC472_000175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725552.1"
                     /translation="MIDINWKKFEVKHPKATDAFESLCYFLFCRKYGLNEGIRTDFNQ
                     VGLETEPIKYTDGKYYGFQSKFFDKNVSYDNIYDSIKKALDNYDNLDYIIIYINKECQ
                     TSCKSAKKIEDMCDSRGIKVEWYLPNNFKLALNQPNNLDLAEFYFGATNVRGLLADSK
                     SIRMTTLLQSKEYLELNLHNYEKTLTTTEYCKEVLSSKKKLHLVSGAAGTGKSVCMQK
                     MLYIYGGFDEETKNKQLEKVKSLGAICIYINLNNTALESLENIVLNYKNTFFADSDNN
                     NFIYLFDGIDEVPTSHITSTVLFIENLLEKENTKKIIISSRLSSYNKYILKASINEIV
                     EYSIENLKEDQVRDYFENKGNQEKVNKLNEVVSNNDNFFEHVTDILTVSLLWDQINNI
                     NGKSFFTNLMDLSVSTIINDIHHRKYLESLNLPNPKEKAIIEINKNISFYLFEKDIFN
                     FTYEDLHKLIGEIYPRCDYISLNKIIAYIAESFFDTSMTTGNYIFSYKHRRFSEYFTI
                     LKLEDKIRNDLNYLRENNIIINHDLFESMLIPYLQIKACNKKDLPLAFQIGLFNVYLG
                     NDKAWGIDKEFYIWSDWIIYAIAGLDNEIFESIMLDSSLPISKFFNEVPKKIKALLSS
                     KSKKNLSYNDELRQNFKIYVVLISLMHKFGKLEFLSVLLQDYKEIRRLCKENKFCFYS
                     TSNKDNFLVWRSIIYINTVILNDNIDELISLVLQNSKDTNIDGLLKDRTSMNILHLNS
                     LYYNMLIYHKNKCANMIENMNINQLSIFAITATNPECLNNIMKDKSLIEKIKQVLEDE
                     ISNENLSGVICLALKKFLGGSLVKKEEELVKKYLENIEFIGSSIFWKGHCDISAFILS
                     VFGKGQIKLKIDQEIKRYVNVYKVFLELIDKSCSIERFICCVQKNIFNNTQDSYYIRI
                     LLGKVLSLLNDEDFNIKGSLDYLNDSINDSRILIIYCTMKLYNPSRFKSLLSITELTK
                     LTEPRVYEDVNFTSTSESLFMLSFILSNHDKLLGHELLLKGISNGIMRMNDRKDTIGD
                     YQLLDSLEILLINNWISTEKLIILLKRILKITNKMNLYNIENDTHARVMDIIQKYDFA
                     AAEFYYNKIAHLEECYNLIHYRYALSMIQRGRNIKYIEACLDNIKLSFDRYHQKAEWG
                     SFYYKIEVYLKISASDFYSSYEQKLSFEKACYKIDELEHAGWSRELKKEEYDIYVELC
                     KKYNKKIDIEKEKENEYCYSVKPLEKENQDILEIINSINTEEDLCEFIKRLDGEYYLE
                     NLEINELFIKKCVDINGNIEVIIDMFTRYNYPSSLYYSRNSHNFWMTVLSALKNAKSK
                     NSMMQYLLENGGGHDGFSELIKIYGYLGNKDICIKLFERMLTCVEFLLC"
     gene            92677..93753
                     /locus_tag="PC472_000176"
     CDS             92677..93753
                     /locus_tag="PC472_000176"
                     /inference="COORDINATES: protein motif:HMM:NF025448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HDF2725553.1"
                     /translation="MKINDFIFRFKYRNDEKSLCRVRTFLGRENKIINLITDLEDLNI
                     SKVSLINSIEFACSELIEKNMTCEDAIFIEHTESESLEEQKFDMVTFNPRGGPIWKNI
                     NINEVKKILNCIDDELINTTKKNRNLVETIDRIRYRINPETDFLYKESPEVIKRKLEI
                     KENRISKSKLEDLIKNRSKEQEIARLLKSDLSVFGEVYSFPKDEYICFSEYPIYDRYI
                     DYVVITGRSRMQVYLIEIKGAEFNLVNKDSKFNEKINEADSQISRTLGYIFRNYMAFK
                     DDIHEKRRLVEKGEKIFNSFRGPISKLEVDRQKDITIHSVIIGGVTNDDIKESRLRHD
                     YENKNDFRIKLESWDTFIRKLSNR"
BASE COUNT        37189 a        10248 c        16264 g        30346 t
ORIGIN      
        1 agttgttaaa ataatattta tattagtttt tatacatact tggtataagt ttttcatata
       61 ttgattaaaa ttgatgttga tttattattt ttagtatttt ttcacctttt gattcatact
      121 ttgtagaatg gtgtattttc taaaactgat tataagacaa gttcattcaa attagcattt
      181 ttatatcata taaataattg ttccccctaa ataagattta gatgtatagt attaaaatca
      241 aaaagtataa aataaaattg atgcttaatc atagataaat attaataaaa tatacaaggg
      301 gattgtctaa taaaaagata atctcctttt ttacaaataa ttctaaattg tgatataaat
      361 ctaaaaattt tatcttaaca tttaaccatt taatgataat aattgatata ataaatataa
      421 attaaatata tggtgtactt attttatgtt tttagcaaga aatatagcat ttaaaagata
      481 aattgtgcaa cttaaatcaa agtttaagat gaactattaa catagatata ttaaaaactt
      541 ttgtaatgga gaatatatgt gatatgaata ataaagttaa ggttgcagta ttagatacag
      601 gaatagacaa agaacatgat tacttaaaag ataatttagt tggaggaatt gcttttgaat
      661 gtatgcatga ttatatcttt atatcagata aatttgatga tgaagatggt catggaactg
      721 cttgtgcatc aataataaag aaagagtatg aagatgtaga gttatttgta ataaaaattt
      781 tgagaaaaga tggtattaca aatataaaag ttcttgaaga agcattaaga taccttttag
      841 acactaacat aagattaatt aatttaagtt tatcagtcat aggagtagaa agtgttaagg
      901 gcttatttga agtttgttat gaattgttta gaaaaggaaa aataatagta tgttcactcg
      961 ctaatgattt tgacttaagt tatccagcta tgtttaacaa tgttataggt gtaagaggat
     1021 ttactttaga tggagatgat tcattttggt acaacaaaaa atatgatgtt caatgtgtta
     1081 tggatagcaa ttcatatata agttgtgata taaataattc ctatagatta ccaccaaaat
     1141 gtaatagtta tttatcagca aagcttactg gtaaaatagc taagatatta tcagaaaaac
     1201 caggcataac aatatctgat ttaaatgata agcttgaatc attagcaact agaaatcatt
     1261 gggatagttg tgattttgat gagtgtagta gaattgtaaa ctttaaattg gatttgtatg
     1321 acaaagaaaa tgctcttctt atgaaagttg ctgacgttgt tagggattgc ttagatattg
     1381 aagataataa tgaaaaactc tttaagggta gcctttttaa taaggagata ggcttggttt
     1441 atgataattg ttttaatctg ttgaaaaagc tagagcatag atttgacata aagtttaatt
     1501 atatggacat ctcaaaatat gacttagttt caatatatac actgactgaa ttagtggaaa
     1561 gatataaaaa cttataaata aagatttgta gctaaattta atgtagaagg tattaaatta
     1621 tatgtattct ttgtcggtag attaaaagta aaaaatatac tataattaga tagcataaga
     1681 gaaaatgcca atacaggggg agttagataa tgaaatatga agaagcatta gagtacatat
     1741 cacaaactaa taaattcggt ataagattag gattagaaaa tatcggaaag ttattagaat
     1801 tattaggaaa tccacaagaa actttgaata taatacatgt agctggaact aatggaaaag
     1861 gttctgtgtg ttcttttgtt tctaatatac taagagagtg tggatataaa gttggtttat
     1921 atacatctcc atatttagaa acttttactg agcgtataag agtaaatgga caaaacatac
     1981 cacaggatga tgttgctaga ataattgagt taataaaaga aaaaatagag attatggtaa
     2041 aagaaggcta tgcatatcca acagaatttg aagtggttac tgcaatggca ttttattact
     2101 attcagaaca aaaggtagac tttgtggcat tagaagttgg tttaggtggc agatatgatg
     2161 ctacaaatat aataacaaaa tcacttgtaa gtgtgatagt atctataagt ttggaccata
     2221 caggtatact tggagataca atagaaaaaa tagcgtacga aaaggctgga ataataaagg
     2281 aaaatggagt agtactagta tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag
     2341 tttgtaaaga gaaaaaagct aaatatatag aagttgattt tgatgatata aacataaaaa
     2401 aatcagatat aaattctcaa atatacgatt gtactgtaat gaaagaaact tatagagacc
     2461 ttgaaataaa attgataggc gaacatcaaa taaacaattc tatcttggca attagtgtaa
     2521 taaaatatct aaaagacata aataaattag caaatataaa tgaagaatct ataagaaaag
     2581 gacttataaa tactaaatgg ccaggaagaa tagaaaagat aaaggaaaat ccaattttta
     2641 taatagatgg tgcacataat gaagatggag caaaatcttt ggcaaaagca cttgataaga
     2701 attttaaagg tagaaaatta accttattaa taggtatgtt agaagataaa gatattgata
     2761 gtgttttaga gatattgttg tcacatttta ataaggtaat aacaacaaca ccaaataacc
     2821 caagggccat aaattcagat atattaagag aaaaagtatt aaaatatgta gatgatgtaa
     2881 catcaaagca tgagatagag gatgcagtaa attatacatt agaaacatca agtgaggatg
     2941 atattataat aagtgcaggt tcattatata tgattggtac agtgagaact ttagtaaaga
     3001 aattataatt ttataaaata agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa
     3061 tggacaactc tttttatata aaataagttt ttaataattt tactgatgtt tatataagta
     3121 attatataga ttgacgtata gctttagata attggtctgg gcaagaagta ccttttccac
     3181 cacagtcaat accatttaat ttatcagcaa cttcaagagc attttggcca attattaagc
     3241 ttctaagtcc tgataaatta ccattacatc ctcctataaa ctcagcatca acaatcacat
     3301 tattttcatc aacttcaaaa atcatttctc tacaacatac accacttgga ttaaatgtta
     3361 ctttcattaa aaagacctcc attaaatatt atttcttttt ttaacattaa taataatacc
     3421 aaatatatgc gtaataatgc aactataaag cataatacaa gctaatgaaa taatatatta
     3481 ttgtagtata tgtattgtag agaggagggg ttatatgaaa atctatattt ctaactactt
     3541 atcaaagagt attagcatag ttgattattc tacacttgaa ctagaaaaag aaatagtatt
     3601 agaagataat atatatccac accatttttg catagaaaaa gaaaaaaatt taatatacat
     3661 acctagttca agtaatggga tactctatgt cttagattta agtaatgata aaataattga
     3721 tactgtctct ataggaggaa gtcttagtca agttgtactt agcgataatg aattgtttgt
     3781 ggcaaatgaa gattctaata gtatatatgt actagataaa aacacattaa atccaatagg
     3841 aattattgga gttgataata tgccacatgg atttgacttt gatagagaaa gcaaaaagtt
     3901 atatgtaccg tgtataaact cgatagtatg tatagataca attaataaaa atatacaaaa
     3961 gaaaataaat atggacttta gagcttggca tatagaactt gataaacaaa agaaagaaat
     4021 gtatatatca acacttgatg gaaagcttat aataatagat gaagtaagca tggatataaa
     4081 aaaggtatta tatgaatttt tattaccagt agaaataaga tttaattata gtggcaaaaa
     4141 agtttatgtt gctgatttgg gttataataa tgtaagaatt ttagattata caacgagtag
     4201 atacattgga aatattgaaa taaatgggat tccccaagga ttagaaatat ctaaagatga
     4261 aaagttacta tttgtatcag atacacaaga aaattcagta aaagtttatg aaactgcaaa
     4321 taacaaatta ataaaagaaa tcaaggtagg aaaagagcct acaactataa tttgtttgta
     4381 ggctttaatg tatgtctctt ataagtaaat cgataacttc ggcatacata taagatgcat
     4441 tatttttcca gttattgcaa ataagttttg cttgtttctc tgatgaaaca tttagattta
     4501 aatctattaa gttagactga ttttctatga ctctaaggtt gacaataaat tcattatcac
     4561 tttgttttac aaaactagac tttacttgag tatctgctag tagattttct ttgttagaat
     4621 ctatatactt gtctattttt tcagtaacac tagtaggtat tcgattaaag aagtattcta
     4681 gactttctac gcctctttga gtaagagcat aatattctct gtcagagtct ttatatgtag
     4741 tcaaaaactt agattccata agctgagata aaagctgttg taatgaaaaa taattcatca
     4801 tctcagtctc taatactact tgagttattt gagaattagt taaatccatt ttaatttttt
     4861 ctaatatata taaaattaaa agtttgtgat aggctaattc ttcagacgag ttttcaaaca
     4921 taaatatcat cctccgtcaa actatttcta tatataatat attaaactaa tttgtacaaa
     4981 attaaaagaa caatttgtat tgtattatta atctttaata attcatataa aaacaatttt
     5041 ataaataaat atagagaaac agggctgcta attagcagcc ctgttgatac ccaggtgtca
     5101 aaatttacta tttattttcc agccatttgt ctttcagcca tttcaactaa tttcttagtc
     5161 atatatccac caacatatcc attttctcta gctgttaagt tacctttgtc tatgttttca
     5221 tagttagata aacctatttc gttagctatt tctaatttca tttggtttaa agcagctttt
     5281 gcttctggaa ctactgttct gtttgaattt gacatagtaa atttcctcct caaataatta
     5341 gtttatgatg tctaacaaca cttttattta tgtgatgtta atactaattt ctccaaaata
     5401 aaaataatat atacagaaaa ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa
     5461 atgagtattt tagagagttt aattaggtca atataggttt tattttttta gttattgtct
     5521 tataaattaa atattacagt acttttttga gctgaaaaaa aataaatttt aaatatagaa
     5581 gaaattattt caaaggggga atagtatgaa aagagaagtt aaaaggacta aagctttgat
     5641 atatagtgtt ttattttttt cattattatt agtgttaata tttgtttttg tttatggaat
     5701 aaaatacata tgttctaaat ctgacaattc aaaaatgcct atatataaag ttgatacaaa
     5761 tgagaaaaaa atagctttga gttttgatgt agcatgggga actgataata tggatgatat
     5821 tttaaaaatt ttagataaac ataatgtaaa agctaccttc tttttagttg gtagttgggt
     5881 ggatgataat gaagaaattg taaaagctat agatgaaaaa ggacatgaaa ttggaaatca
     5941 ttcaaatact catgccaatt tgaaggaaat atcaaaagaa gacataacaa aagagataga
     6001 aactacatca gaaaaaatat ataatataac aggaaaaagg actaatttat ttagacctcc
     6061 ttttggggac gtaaacaata aagctatgga catttgtagt gatttaggtt ataaagtaat
     6121 taagtgggat gtagattcta tagattggaa ggagcttgga ccaaatcatg taattgagaa
     6181 agtaataaaa gaatctcaac ctggttcaat agttttattc catgctaata taaatgatgt
     6241 agataactat ttggatacta taataagtcg actaaaaaaa gatggataca gtctagtcaa
     6301 aatatctgat ttgttgtata aagataatta tgtagttgat tccaatggag tgcaaaaaac
     6361 taagaattaa taagtaataa atgtatattt taagtaaagt tgtgataaat tatatagtat
     6421 atgttagaaa agtgtcgata aaaagacggt gtctattttt tgacactttt tatacatgta
     6481 tgcaatatca ataattatag attaaattga ttaaaaaagt gtctaataat tgactttttt
     6541 ttgatgggtt taaaaattta gaagaattaa tgaatgctgt aagacagtat ttccaaaggg
     6601 tataagtttg tttaaaagtt ggcatagaaa ttgctttgtt aataagcgat atgttctata
     6661 gaaataaaca atatgttaag accttataat agaaaaatag cattaacatt atttgaacag
     6721 cttataaaaa aattaatata cttaaggggg cgtactagtt gaacaaacta tattctattt
     6781 tattaaaaca acacgttggt ggtccagata agccagtagt aagtgttgga gatgtagtaa
     6841 aaaaaggaac tctaatcgca gaaccaactg gattaggggc aaatatttat gccagtgtta
     6901 gtggaaaaat aagtgaaata aatgaccaag cgatagtaat tgaagctgat gaagtacaag
     6961 aagatacttt tgaaccatta aaaggagaag gtatacttga cttaattaaa gaagctggag
     7021 tagtaggaat gggtggagca ggattcccta ctcatgtaaa attaaacata gatttaaatg
     7081 gtggaacaat attagcaaat gcagctgaat gtgaaccttt attagctcat aatataaaag
     7141 aaatagaaga aagaccagaa atagtttatc aaggtataaa atatgctatg gaagttacta
     7201 atgctggaaa aggtatgcta gcaataaaaa gtaagcatcc aaaagcaata gaagctttca
     7261 aaaaagtaat aaagccagga gacaacatag aagttgcaga attagttgat atgtacccaa
     7321 tgggagaaga gagagcaata gtaagagacg ttctaggaaa attacttgag ccaactcaat
     7381 taccttcaga ggctaatgca gtagtaataa acgtagaaac actaactaga atagtagagg
     7441 ctgttgagca aaaaagacca gttatatcta aaaatataac tgtagttggt caattaaaca
     7501 gtggaaaaga atctatagtg tttgaagatg tgccaattgg aacaactgtc ggagaattaa
     7561 ttgaaagagc tggtggaata aaaggtgaat atggtgaaat aatcctagga ggacctttca
     7621 ctggtaaagc tactacttta gatgctccaa taactaaaac tagtggtggt ataatagtta
     7681 ctatgccatt tgttaatgaa aagagaaaaa tgggtctttt agtttgtgca tgtggaccta
     7741 atgaagaaag aatgagagat atagcaacta aaatgggagt tactgatata gtatcagttc
     7801 aaaaatgtaa acaagctcaa gaaattaaag gtgcattaaa atgtgaaaat ccaggacact
     7861 gtccaggaca agctcaaaaa tgtattgaat ttaagaaagc tggagcagaa gttatattaa
     7921 taggaaactg tactgactgt agtaacacag taatgggttc tgcaccaaaa ttaaaattag
     7981 gtgtatatca tattactgac catgtaatga gaacagttaa tcacccatta ataagaagaa
     8041 taaaaggtga cgtaagatta aaaataaaat aatagataaa tagaagttag acatctttag
     8101 aagtaaaact taaaagttac ttctaaggat gttttttaat gtattttgag ggcaatatat
     8161 atattgtaaa ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataaca
     8221 atagctatta caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta
     8281 atatgcatat tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa
     8341 gatactctat agtatctttt ttattataaa aactaatttt tgttttttat tagataagta
     8401 tctaggatac taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa
     8461 aagaaaaatt aaaaacctat ttatagaaac tgtgaagtat atcttaaacc tgggcactta
     8521 aaagatatat ggagttagta gtgcaacctg ctataaatat aaataggagt aacttttaat
     8581 tgtcaaaaat atataaaata aatacataaa taagtaattg atagaagata gatataaata
     8641 tatttttgaa atgctccctg cattcataac gagtgtagag tggcagatat ctgcccaaaa
     8701 ctcctttaat tgctggaagt tcctaaagct aactaaacca caacataatc atgaaataag
     8761 gataagtgtg atagagtgtg catttaataa gcacttgagg ttacgaaagt agaaaaaatt
     8821 agttagatag cataaggtta aatcctaagt gccttgaaaa tggataatca gcaggtaagc
     8881 ttcgaaaaga agaaacttca acgactagag agaaatctcg tacctacaag tgtggggaaa
     8941 tggggagtac ctaatttatg gttaggtaat gagatagtct gtgcttaata gaaatattaa
     9001 gaagttcata ggagaactgg ttaggagtaa cgaccctaat tgaacacact aaagatgtat
     9061 tattgaataa gattcatata atatttaata attttaatta tattaagtga atacaatata
     9121 tgtttttagt atcaggagaa ttttgaaaac aaaaattaaa aaatcaagta taatcagttt
     9181 agtaattgct tttagtatgg tatttactgc atttacacca atagtatctt atgctgacga
     9241 agtgactagc aatgatacaa tacttaatgg agaagaacaa tcaaatggtc agacaccaga
     9301 tgtagagaaa ccatcagacg gtcaggtacc agatggggag aaaccatcag acagtcaagt
     9361 accagatggg gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagatgg
     9421 tcagatacca gatggagaga aaccatcaga tggtcagata ccagatggag agaaaccatc
     9481 agatggtcag ataccagatg gagagaagcc atcagatagt cagacgccag atgtggagaa
     9541 accatcagat ggtcagatgc cagatgtgga gaaaccatta gatgagcaaa ctccagaaga
     9601 agaaaaacca ttggaagaaa aaatagttat agaggaaatg agtttaaaac aagatattga
     9661 taaaattctt gatatgactt tatctcaaat aaataaaata gtatataact tttgggaaga
     9721 tgaagaagat gtaaaggcag atgaacaatc agaaattaac caagttttta cttcaaaaga
     9781 ctcatttatt agcttatggt atgacaaaaa agcaaaagta aaaaattctt gtttactaaa
     9841 agaaatagat ggttcagata gaccttatca tgatttaaga tttgatgata ttacaaagac
     9901 tttaactttt gactatgtaa taaagggtct tgtaggagca agctcaaaag acgacaaata
     9961 tgtaatagaa ggtgaagatg gagagcaaac tgcattttgt tacaataatc atttaagacc
    10021 accaagtagt aatggaaaga gtccatattt accagcagaa gattttaatg gtcaagaaaa
    10081 ccaaaatgaa gaagcagtta aatctatact atatgcaggt tctgaatttg atggatttgg
    10141 atataagcaa caatttaatt taggtggaga agaaaatgaa ttgatgactt actcagctac
    10201 tcaaagtgca atttggatta ttcttggaca aatggatgaa caagaaagac ttaagcagta
    10261 tcaaggttct ataaaccttt gtgataaact tatagaaaga gcaaaaactg aagaggaaag
    10321 agcggagtat cagaagagga aagaagtagc gataaataca aaagcttatt tagaagcatt
    10381 gttaaaagca ggtcgtgaag agttaaagcc aaatgataca ggaaaaccta gtttgagtaa
    10441 tggtttaact aagattaact ttgaaaaaaa tgaagatggt acttatgaaa cagaagctgt
    10501 agccttagta ggttatagtg gtgtagtaaa gttaaaacta ccagatggag taactgctta
    10561 tgatgtagat ggtaatataa taggtacagg cgaagtagaa atttcaacgc aacagaaatt
    10621 taaacttaag agtgttggaa aaccagatgc caaagctaat atttctgctg tatcttatga
    10681 ttatattttt ccaaaagcta ttcaatatta taaggcagtg cttgatttag gacaaaaaga
    10741 ccattcatct tcattaccat ctagcaagca aaatttatta agttatacaa tagagaaaaa
    10801 gaatggtcaa gaagttaact ttaatatagg tttaccaaca gatgatgatg ttgtaaatcc
    10861 accagtgcca ccaatagatg atgatgttgt aaatccaccg gtgccaccaa cagatgatac
    10921 aaatggacat aagccaaaac catcaccacc aatagatgat acagtaataa atccaccagt
    10981 accaccaatg gatgatacaa taataaatcc accagtacca ccaacggagg atgcagtgtt
    11041 aaatccacca gtgccaccaa tggaggatgc agtgctaaat ccaccagtgc caccaacaga
    11101 tgatacagtg ttaaatccac cagtaccatc agtaagtgat acagtggaaa aaactccaga
    11161 actatcaaga gatgatacaa taataaaatc accaaaaaca ggtgatgaaa ctcagctaat
    11221 gtcatatgta tttattagtg tcattgcaat ttgtggatta gcgtatcaat gtaaaataaa
    11281 aagaaattaa tctagattat tattaagttt aaaataaaaa gtctcaaatt gatttataag
    11341 tcattttggg gcttttttat gtaaatataa tatgcaaata tagtttgttt aaaaatatct
    11401 atcttaatta tattcttaaa tttatgattg gatacaggca ttcattaatt gtttgatgaa
    11461 tagaaaaatg ttttgaaggt gaaaatagta ttgaaaaaat aatatactaa gggtataatt
    11521 catttgattt catttgtata aatttaatta taaattttaa agtgattata taaatttaaa
    11581 tgctaattac ttaataaatt ttaataataa ttatttaata aataattatt ataatatgct
    11641 agaataaata tatataaata agttttgatg aggtactact ataaaggggg gactttatgt
    11701 tttcgatacc agaagtaaaa aaagtagaag aggtaatgga tacaaaattt acaactatag
    11761 acgaagacac aagaattgaa gatgcaataa aagaaatgat aaaaagcaac acaaaaactc
    11821 taatggtaat agattcatct gaccaattaa aaggcattat atccatgacg gatatacata
    11881 atctttatga gatgcataaa aagtatgaag gtcaaccagt aaaacttata atgaagaagg
    11941 atgtaatata tgtcaacgaa ggtttaacct tagatgaatg tagagatata atgatattaa
    12001 aaaatatagg gattcttcct gttcttagag ataataaaat tattggagtt ctaaaacaag
    12061 agcacattag ggactatcta tatatgcatt tagaagatta tggattaact ttgaagtata
    12121 ttataggtca aattaaagaa ggaatttgtg ctataaataa cgaaggtgtt gttatcttgt
    12181 ggaataagtt tatggaggaa aggtatgata taaagtctga agatatagtt ggtcgaccga
    12241 tgaatgaatt tttggaaaat actatttcag aaaaagtact aaatagtaaa gttggaatga
    12301 gtgacttgta ttttacagat aaaaaagaaa atatgtatgc attagttcat gcaaatccta
    12361 tattttataa agaagaattt atcggtgttg tatgtacaga agttgatgtt acagaagcta
    12421 agatattagc tcttgaactt gaaaaagtaa atgatacatt aaaatatcta aagaacgaag
    12481 tcaaaaattt atctaaaggt agttttgata agattttagg taaaagttat aaattggaaa
    12541 aatcgaaagc gattgcaaaa caagtggcta gaacaaatag tagcatattt atttggggcg
    12601 aaagtggtac aggtaaagaa gtatttgcaa gagctattca tgattatagt gagagaaaag
    12661 gtcaatttat acctgttaac tgtagtgcca taccaaatga gttatttgaa agtgagtttt
    12721 ttgggtatga atcaggtgcg tttacaggag caagtaaaaa gggtcgaata ggtatatttg
    12781 aacttgcaaa agatggaact gtatttttag atgaaattgc cgatttacct cttagtatgc
    12841 aagcaaagtt gcttagagta ttacaggaaa aagaaataag aagagttggt ggagatacca
    12901 caataaaaat aaatcctaga ataatatcag ctaccaataa agatttgagt aaaatggtaa
    12961 aggcagaaaa atttagagaa gatttatact atagattaaa tgttgttgaa ataaagattc
    13021 ctccacttag agagagaaaa gaagacatag gattattggt tcatagcttt ttagaggaga
    13081 tatgtaaaca aaataataag cctgttctta caattagcaa agatgtaata gatatatttc
    13141 agaattatag atggaaaggt aatattagag aattaaaaaa tacgattgaa aatattgttg
    13201 tattgtcaca aaattctaaa atagatgtgg atgatgtacc aagttatatg atggattcta
    13261 caaataatag tactgaagaa gaagagtatc ctttagattt aacgaaggca actcaaaaaa
    13321 ttgaaataaa gaatataacc aaggcactta aaatgtctaa tggaaataaa gctaaagcag
    13381 ctaagatatt aaatataccc agaacaactt tgtattataa gatagaccaa tacaaaatag
    13441 atgtgtctaa aatatgacac atgactaaat tttgacaaaa tgataaactt taatttaaaa
    13501 cctagtaaag ccaacgcttg tataagtgtt gtgtatatag atgtgttaaa aaaaatgaaa
    13561 aagtgttgat taattgacac tttttttgtt tgagaaaatg tttacaaata caaaaaataa
    13621 catattttaa ataaatattt atataatatt tggctaaaaa tagcgataga tgaagaatct
    13681 attatagaaa aaatcttttt gttaataagt tggcatagga attgcttata atataaagtg
    13741 actttaagtg agaaaaaaat aatattaatc ataacaaatt atgtaggttt taatttactt
    13801 aatttctcag ttaaggggtt acctgttgag gaattgagat ttaaaataat aaataagaaa
    13861 ataatgtaag gaggtatttt atactatgtc aataacttta gaaacagctc aagcccatgc
    13921 aaatgaccca gcagtttgtt gttgtagatt tgaagcggga acaattatag cgccagaaaa
    13981 cttagaagat ccagcaatat ttgcagactt agaggattct ggattattaa caataccaga
    14041 aaatggatta actataggtc aagtactagg agctaagtta aaagaaactt tagatgcact
    14101 ttctccaatg actacagata acgtagaagg atacaaagca ggagaggcta aagaagaagt
    14161 agtagaagaa acagtagaag aagcagctcc agtatcagaa gcagcagtag ttccagtaag
    14221 cacaggagtt gcaggtgaaa cagttaaaat acacataggt gaaggtaaga acataagctt
    14281 agagatacct ttatcagtag ctggtcaagc aggagttgct gctccagtag caaacgttgc
    14341 tgctccagtg gcaagtgcag cagcagaagt agctccaaaa gttgaagaaa agaaactttt
    14401 aagaagctta actaaaaaac actttaaaat agataaagtt gaatttgctg atgaaactaa
    14461 aatagaagga actactttat acatcagaaa cgcagaagaa atatgtaaag aagctaatga
    14521 aactcaagag ttagttgtag atatgaagtt agaaataata actcctgata aatatgaaac
    14581 ttacagtgaa gctgtattag atatacaacc aatcgctact aaagaagaag gcgaattagg
    14641 ttcaggtata actagagtta tagatggagc tgtaatggta ttaactggta cagatgaaga
    14701 tggagttcaa ataggtgaat ttggttcttc agaaggtgag ttaaatacta ctataatgtg
    14761 gggtagacca ggtgctgctg acaaaggtga aatattcatc aaaggtcaag taacaataaa
    14821 agcaggaact aacatggaaa gaccaggacc tttagctgct caccgtgcat ttgactatgt
    14881 aactcaagaa ataagagaag cattaaagaa agttgacaac tctttagtag ttgatgaaga
    14941 agtaatagag caatacagaa gagaaggtaa aaagaaagtt gttgttataa aagaaataat
    15001 gggacaaggt gcaatgcatg ataacctaat attaccagtt gagccagttg gtacattagg
    15061 agctcaacca aacgttgact taggaaacat gccagttgta ttatctccac ttgaagtatt
    15121 agatggtggt atccatgcat taacttgtat aggacctgca tcaaaagaaa tgtcaagaca
    15181 ttactggaga gagccattag taataagagc tatggaagac gaagaaatag atttagtagg
    15241 tgttgtattt gttggttctc cacaagtaaa tgctgagaaa ttctatgtat ctaagagatt
    15301 aggtatgtta gttgaagcta tggaagttga tggagctgta gtaactactg aaggtttcgg
    15361 aaacaaccat atagatttcg catctcacat agagcaaata ggtatgagag gtataccagt
    15421 agttggtgta agtttctcag ctgttcaagg tgctctagtt gttggtaata aatacatgac
    15481 tcacatggta gacaacaata agtctaagca aggtatagag aatgaaatat tatctaacaa
    15541 cactttagct ccagaagatg ctgttagaat aatggctatg cttaaaaatg ctatagaagg
    15601 tgtagaagtt aaagctcctg aaagaaaatg gaatccaaat gttaaattaa ataacataga
    15661 agctatagaa aaagttacag gagaaaaaat agtattagaa gagaatgagc aatctctacc
    15721 aatgagtaag aagagaagag aaatatacga aaaagacgaa aactaaaatt tagatactaa
    15781 ctaagattta gacaataaca taaatttaga tccaagttta aaacagtata gagaggtaga
    15841 attataatgg ataggttaaa atatatctca tcagagactt tttatgaagg tattatagtt
    15901 gacatcaaag gtggtggagt gactatagac ctaaaaggta gacttggaca atttaaaata
    15961 cctaatagaa tgcttataac tgactacgaa cttaagatag gtcaagaagt tggattcatg
    16021 ctaagctatc ctgaggtatt aagtccagag cctaatcaag agtatgtaga aaatattagg
    16081 agagaaaaag aaaaacaagc agaaatgcaa tcaaaacaag aagaaaaata ataaataatc
    16141 gtttgaaaat agaagggaga ggaaatatat gagccttaca acagtacaag gacttcaatc
    16201 tgaaatattc gttccaataa cacctcctcc agtttggact cctgtaacta aagaattaaa
    16261 agatatgact atagctttag ctacagcagc tggtgttcat ttaaaatctg acaagagatt
    16321 taatctagca ggagatacta catttagagc tatacctaat acagctacag ttgatgaaat
    16381 gatggtatca catggtggat atgacaatgg agatgtaaac aaagatataa actgtatgtt
    16441 ccctatagat agattacatg aattagctgc agaaggattt atcaaaggag tagctcctat
    16501 gcactatgca ttcatgggtg gtggaggaaa ccaacatgtc ttcactgaag aaactggtcc
    16561 tgctatcgct gctaaactta aggaagaggg agtagacggt gtagttatga cagctggctg
    16621 aggtacttgc catagaactg ccgtgatcgt gcagagagca atagaggaag ctggaatacc
    16681 tacaataata atagcagctc ttcctccagt agttagacaa aacggaactc ctagagcagt
    16741 tgctccatta gttccaatgg gtgctaatgc tggtgaacca cataatatag aaatgcaaac
    16801 acatatatta agagatacat tagagcaatt agttgcaata ccatctgctg gtaagatagt
    16861 tccattacca tacgaatata aagctcacgt ttaaaatgta atttatattt gaaaatatag
    16921 aacccttctc ctacacggtt aggagagggg ttttaagaac aaaggtggat attatggaag
    16981 aaaaaataat gagaagactt gttataaaac cgtttcatat gaatgaagtt aatttcggtt
    17041 caaaaacttc tataaagaaa gatgtactta caatagattt atcaagtata gatgaaataa
    17101 aagaaagaga agagttaata acagacataa aagtagacat tataaaacct ggagattatg
    17161 acagagaaat taatactatt atggacatta taccaatatc aacaaaagtt ttaggcagac
    17221 tgggagaggg tattacacat actttaacgg gtgtatatgt aatgttaact ggtgcagatg
    17281 aagatggaaa tcaaatgcat gaatttggtt cttcagaagg aattttgaaa gaccaaatgg
    17341 tatttgggag atatggaact ccatcagttg aagattatat aatccatgta gatgtaactt
    17401 taaagggagg tttacctttt gaaagaacac ttccacttgc agcattcaga gcatgtgatg
    17461 attttataca agaaataaga gcgtctttga aaatggaaga cggaagaaat gctactcaag
    17521 ttcgtgaata ttttgacaaa attcgaccaa atgcaaaaaa ggttgtcata gtaaaacaaa
    17581 tagcaggtca aggtgctatg tatgacaatc agttattctc aaaggaacct agtggatttg
    17641 aaggaggaac atcaattata gatatgggaa atgtacctat gattatatct cctaatgaat
    17701 acagagatgg tgcccttaga gctatgactt aagagaggtg gatattatgg gaataggacc
    17761 ttcaacaaaa gaaacatctc ttcatcactt tagagaccca cttttagaca tagttgaaag
    17821 tgataaggat gtagatttac ttggagtaat agtagtagga actccagatg gaaatgaaaa
    17881 taaaaccttt gtgggacaaa gaacggccgc ttggctggaa gccatgagag tagatggagc
    17941 tatagtttca tcagatggtt ggggaaactc acatgttgac tacgctaaca catttgagga
    18001 aataggaaaa agagatatac ctgtagttgg agttacattt aatgggactc aagctaagtt
    18061 tgttgtaagc aatcaatata tggataccat agttgatatg aacaaatcaa aagaaggtat
    18121 tgagacagaa gtagttggag aaaacaacac aaatgaaata gatgctaaaa aagctttggc
    18181 atttttaaaa cttaaaatga gaaaacatgg ataaaaaatt taagtaaggg aatgagtttt
    18241 gtgggaattt ctacatcaat gaaaaacaca tcacttcata tatttagaga cccgttacta
    18301 gatttgatag ataatgattc tgacatagac ttgttaggag tggttgtagt tgggacatca
    18361 gagcacaatg aatggaaaac atttttagca actagacttg gaagatggat tgaatcattg
    18421 cgacctgatg gagtgattat aactttagat atggcaggaa accaacatat tgattttaca
    18481 aatgctatag cagagtttgt aaaatcagat atacctacag taggtcttac tattatggga
    18541 gcagatggat tagtaattac caatccatat ttggataaag ctaccataat tgattataaa
    18601 aagacaacag gctacataga aacagaagta gttggagaca atcacatgga tgaagttgat
    18661 gttaaaaaag cattggcttt cctaaaatta aaaatgcgta aagacgcaaa ataaacctaa
    18721 gggggaattt gatatgaaat ttagcagaag tatacaagct atagactctc atacagcagg
    18781 tgaagcaact agaatagttg taggtggaat acctaacata aaaggaaatt ctatgcctga
    18841 gaaaaaagaa tatttagagg aaaatctaga ttatttaaga actgctataa tgttagagcc
    18901 aagaggacat aatgatatgt ttggttctgt aatgactcaa ccttgttgcc cagatgctga
    18961 ctttggaata atcttcatgg atggtggcgg ataccttaac atgtgtggcc atggtacaat
    19021 aggagctatg acagcagcta tagaaacagg tgtagttcca gcagtagagc ctgtaactca
    19081 tgtagttatg gaagctccag caggaataat aagaggagac gttacagttg tagatggaaa
    19141 agctaaagaa gtatcattct taaacgtacc agctttctta tataaagaag gtgttgaagt
    19201 tgacttacca ggtgttggaa ctgttaaatt tgacatatca tttggtggaa gcttctttgc
    19261 tataatacat gcaagtcaat taggtttaaa aatcgaacct caaaatgctg gtaaattaac
    19321 tgaactagct atgaaactta gagatataat aaatgagaaa atagaaatac aacatccaac
    19381 tttagctcat ataaaaactg tagacttagt tgaaatatat gatgagccaa ctcatccaga
    19441 agctacttac aaaaatgtag ttatatttgg tcaaggtcaa gttgacagat ctccatgtgg
    19501 aactggaaca agtgctaaat tagctacttt acatgctaaa ggtgaattaa aagtaggaga
    19561 aaaattcgta tatgaaagta tattaggaac tttattcaaa ggtgaaatag ttgaagaaac
    19621 taaagttgca gatttcaatg ctgtagtacc taaaataact ggttctgctt atataactgg
    19681 atttaatcat tttgtaatag atgaagaaga cccacttaaa catggattta ttcttaaata
    19741 agtaataaat aaattaggaa attaaaatag aatagtagcc acttgtcggt tgctattcta
    19801 tagcttttag gctataagat tcagaatata tgaaaaaata aaaaaatagg aggatggatt
    19861 atggtagcaa tcttaaaggc attatatgta gtattcgcag caatctttgc agttgtgttt
    19921 ggtaaagacg tagctaaagc aaacaaagag gggaaactag aagaacaaag tactccaaaa
    19981 gttgctgtaa caggatttat aacagacttc tttgatacat taggaatagg gtcatttgct
    20041 ccaacaatag caatgtcaaa ggctttaaag ttaaatatac cagataaaaa aatgcctggt
    20101 acattaaacg tagcacatac tattccagtt gttacagaag cattcatatt tacatcaatc
    20161 ataccagttg atggtgttac tttagtatct atggttgtag cagcagcagt tggttcatat
    20221 ataggtgcag gaataatagc taagatggac gagagaaaaa tacaacttgt tatgggtgta
    20281 actttagcat taacagcaat attaatgtta ttaggtcatc cttggattaa cgtattacca
    20341 ggtggaggaa atgaattagg tcttactggt gttaaattag taataggtgt aataggtaac
    20401 tttatactag gagctttaat gacagctggt ataggtttat atgctccagg tatggcaatg
    20461 gtttatttct taggaatgtc tgcaaaagtt gcattcccaa tagttatggg ttcttgtgca
    20521 ttattaatgc cagttgcaag tatgaaattc ataaaagaag atgcatatac taaaaaagca
    20581 tcagttataa tagctatatg tggtttaata ggagttttcg tagctgcata tatagtaaaa
    20641 actctaccaa tggatatatt aaaagcatta gtaatagtag ttatagctta tacttctgct
    20701 acaatgataa tggctgcaaa taaaaacaaa aagactgctt agttaaagac tacttagtgt
    20761 ttattaaata ttacagctaa taataaagat tataaactgc tttaaaatat tgaattaata
    20821 ttttagggca gttttttatt tactaaatta atataaagca attaacatgc caattatgta
    20881 atatatatag tgatttagta gatttaatgt aaaaaacgtt gtatttttaa agaataaagc
    20941 ataatttaaa gctaaatatt taaattttat aatcaataag ttcaataaat gttcatatat
    21001 taatacagat taataaaaat ataacctgta ataagtctaa tataacttat aattaagaaa
    21061 aataagttta taatgactca atattcatct gaattatttg aataaattta attcgttgaa
    21121 gttgcttata tagagtgaat aaatttaagt tatatatgac ttattaacta tataatagga
    21181 tttttataat taatataaaa ctgaaatagg tagtttaatt agtatatgtg gtacaaaaag
    21241 ataataattt tataattata aaagtgaaat attaaatgaa atttataact aaataaaaac
    21301 ttttaaacag caatcaaaga tttgtaataa aatacatccc ttataaatat atttattata
    21361 taagctgata ccagaaatat aagaattaac aattgtggag ggataaaata tgatagctgt
    21421 taaagatgag aataacgtag taaacttaag aggggagtta gacaataagc tagaatttag
    21481 ccatgagata tttggagaaa aattttataa tatgaaaata aaaataaaca gactaagcga
    21541 ttcttttgat atacttccaa tgactgtatc agaaagactt ctccaagata ttgaccttga
    21601 taaacaaaat ttagtcaatg tcgtaggcca attaagatca tataacaaaa cattaaataa
    21661 taaaaataga ttagtgctta cagtgtttgt cagagaaata aaaagcatag acgaggaaaa
    21721 taaagaccct aatagtatat ttttagatgg atatgtatgt aaggaacctg tgtataggaa
    21781 gacaccttta ggaagggaaa taaccgattt attagttgct ataaacagac cttataataa
    21841 atctgattat ataccatcaa tagtttgggg tagaaatgct aaatttgcaa agaatctaaa
    21901 ggttggtgat agaatacagc tttggggtag ggttcaaagt agagaatatg agaaaaaaat
    21961 agatgaaaat aatgtggtca aaaaaatggc atatgaagta tccatatcga aaataaaaaa
    22021 attggatgaa aataacaatg aataaacatg aaaaatagac tgatttttat gaataaaatc
    22081 ctaataaatt tggtataata tcatattaaa atgtataaag attgtttaaa aaaatactaa
    22141 tgaaatatct cttgaatagt tgctcgccat ttattataat gtaatctgta cataatttat
    22201 aattgaggaa atgaagaggt gaagcattgt gaaaagcata ttatgtcaga taaataaaca
    22261 gccggttaaa gctaaatata catctgatga attagaatgt tttttggata caattgagta
    22321 taatgtaggt gataaaatat tgcttgttgg tcatatagga aacctaggca agagattgag
    22381 aggtcttgga acttctgtaa ctatactgga gaatagtaat tatgaagatg tctgttattc
    22441 tctagtgtat aatgaaaatt gcaatgtagt taaaggtaag ttagagtttc taccatttga
    22501 tgataatcag ttcaacaaga taataatagt tgaccaattt aatgttatta cagattgtga
    22561 aaaagcttca tctgaaatac aaagagtgtt aaagggcaat ggagaggtta tattagagga
    22621 tttaaattta aagaatttaa aagttaagtt aaactatctt aaacataaat tttgtggttc
    22681 tgttgcaaag attcactatc cacatgaagt atttaatata ttttcaagat taaactttga
    22741 tggtatgatt aaagagtcaa ataaattaaa atatatttat gtgggaaaaa gaagataaat
    22801 gagttaaaat ataaatatgg atataatata aaagactagg ttaagcctag tcttttgtta
    22861 taaggagatg tttagatgga aataaattat gagttaataa taattgttat gctctttgta
    22921 atcctgatgt caatccaatt tactttaaat aagatatact tgattcttaa agaaataaga
    22981 caaatactta tgttaaagaa aaataaggaa tgatgattat gataggttta gaatatataa
    23041 aatctgatat tcaaaatata gctgaagcta tagtatcagt actcaatata gatgtaacta
    23101 tagtaaataa ggagttattt aggattgcag gtacaggagt atatatagac aaaataggtg
    23161 agaaagtaga taaatatacg gcttttaaaa aatcacttac agaacagatt acaatactaa
    23221 ttgatgaccc aaagagtagt gatatatgta gggaatgcta taaaagtggg gcttgtgtag
    23281 agtttgcaga ggtgtgttgt cctattatat gtgatggcga ttctcatggt gttataggcc
    23341 ttatagcctt tacaaaagaa caagcacaaa ttatagaaaa taataaaagt ggtcttataa
    23401 actttttagg caaaatggca gacctaatat ctaataaatt aaaagcacaa attaaaactt
    23461 atgagcttga attagaaaag aaaaaattag agacactatt aaacaatatg gacaaagctg
    23521 ttgtgtctat tgatgtggat ggaaaagttg ataaatataa ttcaaaattt aaagagcttt
    23581 tcaatataaa agatagtata actggcaaaa acatatttgt cgaattagat tttataaaga
    23641 aaccttctat aaataacttt aagaaggaca aatcctgtag ttttttttat aaaagatatg
    23701 gatatgattc gaagggaata tacaatatca ataaaatagt attaaaaaat gagttaaagg
    23761 gatatgttat tgattttata gataaaagag atgctataaa aaactacaat aagatgaata
    23821 aagattatag aataaaattg gaaaacataa ttggagaaag tgtagctata agaaattcta
    23881 aaaaggaagc cctaatagct tctaaatcaa catcaacagt acttattact ggggaaagtg
    23941 gaacgggtaa ggagctattt gcaagagcca tacacaacca tagcaataga gtagataatc
    24001 cttttatagc tgtaaactgt gctgctatac cggataattt attagagagt gaattatttg
    24061 gatatgaaga gggagctttt actggagcta aaaaaggtgg aaagcttgga atgtttgaag
    24121 tagctcataa gggaagtata tttttagatg aaataggaga tatgagtttg catttacagt
    24181 caaagttatt gagagtatta caagaaaagg aacttaataa aattggaagt aaatcaaatg
    24241 tttcaataga tgtaagaatt attgctgcta caaataaaga tttagaaaaa atggttcaaa
    24301 atggcacttt tagagaagat ttatactata gattaaatgt tatacctata aaattaccaa
    24361 gtttaagaga gagaaaagaa gatatcccac ttataatcaa gcatatggtt aaagagtatt
    24421 caaaaaaatt agataaaaat gttattgaca tagatagcag ggttgttgat gctatgatgg
    24481 aatataagtg gccagggaat ataagagagt tgcagaatat tattgaatat agtgtgaata
    24541 tgtcttcttc ttcaataatc acaatgaatg taattcctca aaaaattaag aatacctctg
    24601 tagaagagtt agaagttaaa agcgatagga taattccact tgatgaacta gagaagattg
    24661 agataataaa agctttaaat aagtacaaag attataaaaa agataaagaa ttaacagcta
    24721 aagcacttgg aatatcaaga gctacattat atagaaaaat tgaaaaatac aatataattt
    24781 catagtaata taataaaaag ctatataaaa tttaaatttt tctaaattca taactttaaa
    24841 gaaagcttta aacttatact ataagtgttt tgcagaatct ttttttatga attaaaaaat
    24901 ggtttttata agttaaaaaa taatacattt gagccgtctc aaaatgatat agaatctcat
    24961 tttgagactt ttttttgtct ttttgcgaaa ttgtcatatg tttatccatt ttaaagaata
    25021 taaatactct atgtaaataa aaaaatacat aaatagaagc agtcatacca atggttatag
    25081 aatataagac taaaaatata aaaagatata aaaaatatgg catgtatttt gctataaata
    25141 tagataagaa attatttaat taattatttg agtactaaat ctaaacttaa ttgggaggtt
    25201 tatatataat gagagatata agaaaagaat tagtggaaat gttaaaggca gaagttaaac
    25261 cagcagtagg gtgtactgaa ccagtagcct tagctctagc atgtgcaaag gcaaaggaat
    25321 tattaggaga agaaatagtt gaaaatcgta tgttagttag tcctagtatc tataaaaatg
    25381 gtatgtgcgt aggtatacca ggtactgaaa gattaggatt aaaaattgca gcagctctag
    25441 gtatagtagg tggacattct gaaaatggat taagtgtgtt agaaactcta acaaaagagg
    25501 aagttaaaat agctgaggat tatatggata atactccact atcaataaca cctgctgata
    25561 caagagagaa agtatttata gaagttgtgt taaaaggaaa aaatcatatt gctaaagtga
    25621 gaataagaac taaacatgat aattttacgt tcttagaaaa agatggagaa gtattattag
    25681 acaatgaacc aaaagtatca gcttcaaatg atgtggcaga aaaagctgaa agtttaatgg
    25741 atacagtaac tatacaagaa ttaataaaaa atgtagaagg aattgatttt aaagatatag
    25801 aatttttact agatggagta aaaatgaatg aagagatggc tgaatatgga ttaaaacaaa
    25861 aaactggtat tggtgtcgga tatggaatta aaaagtctat agaagaaggt cttttaggta
    25921 atgatgttat aaattatgca atgatgttaa ctgctggagc ttcggatgca agaatggcag
    25981 gggtaaaaat gcctgttatg agttcaaatg gaagtggaaa tcatggatta acagcaattc
    26041 ttccaatcgt tgcttataat aaaaaattcc cacaatctga tgaaagacta gcaaaagctt
    26101 tagctatatc tcatctagta actggttata ttaagaacta tacaggaaga ttatctgctg
    26161 tatgtggatg tggtgttgct gcttctactg gagcaactgc tggtatctct tggcttatga
    26221 atggtactga aaagcaaata gaaggagcta tagaaaatat gatagctgac ttaagtggaa
    26281 tgatatgtga cggtgcgaaa gctggttgtg cattaaaatt atcttctgca gcatctgcag
    26341 ctatacaaag tgctataata gctaagcaag attgttttgt accaccacta aatggtatag
    26401 ttggttcaag tgttgagcaa agtatacaaa acttaggtag agtaagtgac aaaggtatgt
    26461 ctataacaga tgagataatc ttaaatgtaa tgaatgatat gaataaagtt gactagtact
    26521 ttataatatg ttttgtatta tgagggataa caaaaatagc ataaataaat ttaattagta
    26581 ccctctaata aaatatgatt atgtataaat ttatgtataa tagaaaaaac ttgttagtgc
    26641 aagttttttc tttttttgtc taaaatatat agtatgtaat ttagtgtgga tatatgaact
    26701 acttaaaata taagatacat tgttaataat tgttgaatga acataaaaac tcaataatat
    26761 tgactaatat tgaataagag tattgaaaaa gtgagcaatc tcttataaaa ttaagaagga
    26821 ttattacaat tggaggtctt tttatggata tagaaacaaa aaaatgggaa gtattgtatt
    26881 cagaagaaaa aattaaagat aaattaaggg aattaggagc aattatagaa aaagattata
    26941 aagataaaaa tcttatggta gtttctctat taaaaggtag ctttatattt tgtgcagact
    27001 tagttagaaa tataaactta ccacttagag ttaattttat gactacatct agttatggaa
    27061 acaatgaaga aagtactgga aaggttaaag tagtttcaga tgtgactact gatatagcag
    27121 gatatgatgt tttggttgta gacgatatta ctgattctgc acttactatg gattttgtgt
    27181 taaaacattt aaaagctaaa aatcctgcta gtttaaaatg ctgtgttctt ttggataaac
    27241 caagcagaag aaaagtcgat ttagtgccag attattgtgg atttgaaata gaagataagt
    27301 ttgtggttgg atatgggttt gattttggtg attattatag aaatgttcct tatatattca
    27361 atgttactga tgaagataga taagcaatta tataattttg tggatgcctt aatatgattt
    27421 ttattaatcg tgttgaggtt tttttattta aaaagtaatt aaagtagtat taaattaatt
    27481 ttatgcaatt taattatcta ttttttataa cataataaaa aaattttaaa taattgatgg
    27541 gaaatgagtt ttttaattgt attaataatt gtaaaagatt ttacaaaaaa gtaatgaaat
    27601 tcaagaatca aagcatatat gttgatataa gcaaatagat acattatcac ataagaaatt
    27661 gtcgattcat ataattatat agggtaatac tcaatattta tagagtatta ctctaacttt
    27721 aaaaaaggag gattaattat atgagtgata tttcaggtcc aagtttatat caagatgtag
    27781 gtccaacagg gccaacaggt gctactggtc caacaggacc gacggggcct agaggtgcaa
    27841 ccggagcgac cggagcaaat ggaataacag gaccaacagg aaatacggga gcaactggag
    27901 cgaatggaat aacgggtcca acaggaaata tgggagcgac tggaccaaat ggaacaacag
    27961 gttctacagg accaacagga aatacaggag cgactggagc gaatggaata acaggtccga
    28021 cagggaatac aggagcaacc ggagcaaatg gaataacagg accaacagga aacaaaggag
    28081 caaccggagc aaatggaata acaggctcta caggaccaac aggaaataca ggagcgactg
    28141 gagcaaatgg aataacagga ccaacaggga atacaggagc aacaggagca acaggtccaa
    28201 ccggactaac tggagcaacc ggagcaaccg gagcaaatgg aataacaggt ccaacaggaa
    28261 atacaggagc aaccggagca aatggagtaa caggtgctac aggcccaaca ggaaatacag
    28321 gagcaacagg tccaacagga agtataggag cgactggagc aacaggaaca actggggcaa
    28381 caggcccaat aggagcaaca ggtccaacag gagcagatgg agaggtaggt ccaacaggag
    28441 cagtaggagc aacaggtcca gatggcttgg taggtccaac aggcccaaca ggcccaacgg
    28501 gagcaaccgg agcaaatggt ttggtaggcc caacaggccc aacgggagca accggagcaa
    28561 atggtttggt aggtccaaca ggagcgacag gagcaacagg agtagctggg gcaataggtc
    28621 caaccggagc agtaggagca acaggcccaa cgggagcaga tggagcagta ggtccaacag
    28681 gagcgacagg agcaacaggt gtaaatggag caacaggccc aacgggagca gtaggagcaa
    28741 ccggagcgaa tggagtagca ggtccaatag gtccaaccgg tccaaccgga gcaaatggag
    28801 tagcaggagc aacaggagcg acaggagcaa caggtgcaaa tggagcaaca ggcccaaccg
    28861 gagcagtagg agcaacggga gcaaatggag tagcaggagc gataggacca acaggcccaa
    28921 ccggagcaaa tggagcgaca ggagcaacag gggcgaccgg agcaaccgga gcaaatggag
    28981 caacaggtcc aacaggggcg acaggagcaa caggagtgtt agcagcaaac aatgcacaat
    29041 ttacagtgtc ctcttcaagt ttagtgaata atacattagt gacatttaat tcatcattta
    29101 taaatggaac taatataact tttccaacaa gtagtactat aaatcttgca gttggaggga
    29161 tatacaatgt atctttcggt atacgtgcca cactttcact tgcaggattt atgtcaatta
    29221 ctactaactt taatggagta gctcaaaata actttattgc aaaagcagta aatacactta
    29281 cttcatcaga tgtaagtgta agtttaagct ttttagttga tgctagagca gcagctgtta
    29341 ctttaagttt tacatttggt tcaggcacga caggtacttc tgcagctgga tatgtatcag
    29401 tttatagaat acaatagttt atatgatgaa aagactataa aataataaac aatataattc
    29461 cacataccaa taaatagaat aatatttaat aggtttatat gatgaaaaga ctataaaata
    29521 ataaacaata tgatttcata taccaataaa taggataata tttaataggt ttatatgatg
    29581 aaaagactat aaaataataa acaatatgat ttcatatacc aataaattag gataatattt
    29641 aataggttta tatgatgaaa agattataaa ataataaaca atatgattcc atataccaat
    29701 aaataggata atatttaata ggtttatatg atgaagagat tataaaataa taaacaatat
    29761 aattccatat accaataaat aagataatct ttaagaaaaa tgattatatt taaaaggtaa
    29821 gcataaaatt tataaacttt tagaatagaa atctcaatca attagatatt caaattgatg
    29881 aatatgaata cctaaataga ttgagattta ttatgtacaa aacttatcct agtatatctg
    29941 acattttata aagtccagat tgcttaccaa ttaaaaattt agctgcaact atagaaccgt
    30001 ttgcaaatac ttcatcagat tgagaatgat ggcttaatgt aagaacttca ttatcacctg
    30061 caaatataac atcatggtcg ctgattatat tgccacctct tatagagtgt attccaattt
    30121 catttggttt tcttttacaa tctctaccat atctaccatg tgtattttca ctttcaggca
    30181 agacttctct aattgcatta gctatcatag tagcagttcc actaggggca tcttcttttc
    30241 tattgtgatg tttttctatg atttcaatat caaaaccttc taacatcttt gaagcatcct
    30301 taactaattt tattaatata tttactccaa gagatgtatt tgatgataat aatataggaa
    30361 tgttttttga tgcttcttct attttattta agtcattttc gtcatatcca gtagtagcta
    30421 atactacagg tgtattattt tttagagcat atgacaatat tggctctaaa gtctcatgat
    30481 gtgaaaagtc tattatgaca tcagtttttt cagttacatc agtcattgtt ttgtaaagct
    30541 ttgtatcact atgatgacct ttttcatcag cagacctagc tacaccacaa accaactcta
    30601 attcattatc tttttttaca cattctgcta aaactcttcc cattgttcca ttatagccac
    30661 taattataac tttcaacata aattcctcct atgaaattaa taaaaatttg ttatatttct
    30721 ataagtttat tttatcacta atataattta tataaaataa tattagtgat aataagctta
    30781 caatttatct tacattaaaa aatttaagtg gatttttcaa aatatagata aatttctttt
    30841 tcattgttta aatgataaaa atatatttga cttcatacca aaaagagtgc cccatgaact
    30901 aaaaagttaa ttttgaaata ccatattcat atatcatatg atttatttgg aaatcaatat
    30961 taatacatta aaataactaa atacatgtat gcataaagat gtataaaaaa tatataaaca
    31021 tatagtatta attttaaatt caacaattta attgttaaaa gtgttattat taaattagag
    31081 atatcttaac ttaatatact gagatgggag tgatagatat gttaaaagaa gaaaaaactt
    31141 atcaagagaa agacgcaaaa gtattcaatg gagtggcagc tttgataatg attttattat
    31201 ttttagtagt ttcaataggt atcatggttt ttgggatagt aaaacttgat agtggattta
    31261 ctggaatagg ttcacttatg ttaattttgg gacttgtatt tatagtagta gattttatcc
    31321 tattttttgg gctaagaatg attaatccta aagaagctat agttctagtt ttgtttggta
    31381 actattatgg aactataaaa aaagaagggt actattgggt gaatccattt tgttcagcaa
    31441 taaacccagc agtatcaaga attaatgtat cgaaatcttc atctgatgaa aaggtagata
    31501 tatcaactaa ttcaaggggc aaaaaagtat ctttaaaaac aatgactctt aataatgaaa
    31561 agcagaaagt caatgattta ttgggaaatc caattataat aggagtggtt gtaatttgga
    31621 aggttataga tgcaacaaag gcagtattta atgtggataa ttataataca ttcttatcta
    31681 ttcagtgtga ttctactatt cgcaatgttt caagacttta tccatatgat gtatctgaag
    31741 atggagatga aaaatctctt cgtggtagta gtcaggaaat agcagataga ttaaaagatg
    31801 aattacaatc aagggttgac attgcaggaa ttgaagtgtg tgaagtgaga attactcatc
    31861 tttcttatgc accagagatt gcagcagcaa tgttacaacg tcaacaagca gaggctataa
    31921 tagcagcacg taaaaagatt gttgaggggg ctgtaagtat ggtagagatg gcattgacta
    31981 atttaagtga aaataatgtt gtgactttgg acgaagaacg taaagcagct atggtaagta
    32041 atctgttagt tgtgctttgt ggcaataaag atgcacaacc agtagtaaac agcagttcta
    32101 tttattaaat tataaaaagt ttaatagttt attttattga tatatagaaa atttagtagt
    32161 tcattttatt aaattataga aagattaatg gttcgcttta ttgaattata aaaagtttaa
    32221 taatttattc tattgatata tagaaaattt agtagttcat tttattaaat tatagaaaga
    32281 ttaatggttt tcactttatt aaattataaa aagtttaata gtttattaca ttgatatatt
    32341 gaaaaattga aggaaataaa aatacttgtc agcaagaaat aaaaatgata taagaatgaa
    32401 taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat tgtaaaaaag
    32461 agggtgcctc tggtgaatta aattcacaca gagacacctt tttaattaat tttttaaatt
    32521 agtatattat tgtttaatta atagtatagt actatttaat tagtctaaat ttctaaggtc
    32581 ttccataagc ttagttttgt cagccgtttt ttcatctttc atttttataa ctttagcagg
    32641 agaaccagca acaacagcac cagcttctac atcagtagtt acaacagcac cagctgcaac
    32701 aacagcacct ttaccgattc taacaccttc aagaataact gcattagctc ctattaaaac
    32761 atcatcttca actataacag gagtagcaga aggtggctct aaaacaccag caacaacagc
    32821 accagcacct aagtgaacat ttttaccaag agttcctcta gctccaataa cagcattcat
    32881 atctaccata gaaccttcac caataacagc tcctatattt ataacagctc ccatcattac
    32941 aacagcattt ttttctatag taaccatgtc tcttattatt gcacctggct caattcttgc
    33001 atgttcactt aaagtatttt ttagaggaat agcagaattt cttctatcat attctacatg
    33061 agtatctgtt atatcatttc catgtttatc taatacttcc attatatcat catagtcacc
    33121 tacaaatata taagaaccat tatttccaaa taccttaaat ttatcagttg attggaagtg
    33181 taaagagtta gtatttacat ataccttaac aggtgttttc tttttaactt cttttatata
    33241 ccttgctatt tcataagcat cgtttaaatt taccatttgt taattctcct tgtatttcat
    33301 tatatttctt ttattatata cttatttgtc aattttagta tatacttact tcttattatt
    33361 tatattttat tttagttagt ttttattgta tagacatagt attaaataaa tttattatat
    33421 atgtttgatt tgtattgcat attattatat atatattgta tatgtttaat ttatattgtg
    33481 tattattata tttatactat tatcccaaca tgctgtccat attataaaat cctggttttt
    33541 gcttaactaa gtatttggca gcagctatag aaccacttgc aaagatacca cgagattgag
    33601 cttgatggct tattgtaaga acctcattgt ctccagcaaa tagagcatca tgttcgccaa
    33661 caatatttcc acctcttata gcatgaatac caacttcatt tggttgtctt tttgcactac
    33721 gaccatatct accataatta ttttcaacgt ttggcaaaac ctcttttata gcgttagcaa
    33781 tcataacagc tgtgccactt ggggcatcta cttttctgtt gtgatgtttt tctattattt
    33841 ctatatcaaa tccttctagt aatttagctg catctttaac caattttact aatacattta
    33901 caccaagaga catattatat gattggaata caggaataac tttagccgct tcacgtattt
    33961 tgttcatttc gtcatcatta tatccagtag tagcaattac taaaggtgtt ttagtcttta
    34021 aaacgtaaga taagatatta tctaaattag agtgatgaga aaaatctata actacatcag
    34081 cttcctcaac tatagtactc atatcttcat aaattttaag gtcgccttca tagctatcca
    34141 gacctctagc tgcaacacaa accaattcta attcattatc ttctcttata gtatcggcta
    34201 aaatcttacc catttttcca tcataaccag ttgctataac ttttaacata atttcctcct
    34261 atgattaagg gctaactttt gaccaaactt actttttacc atgcattaat ctttactatg
    34321 cattaacttt tagtccaaag ttaactaatt ctttctttaa cacttctaaa tttgctgggt
    34381 ccatttctgc tagaggtaat cttaaatctc caacattgaa tcccaataaa ttcattgcag
    34441 tcttaactgg tataggattt acttctataa ataaagctgc gattagagca tccataccaa
    34501 gttgtaattt tctagaacct tcaatatcgc cttcaaagaa tttagctacc aaatcgtgag
    34561 tttcttgagg gcaaacatta gcaagtactg atataactcc ttgacctcct aaagataata
    34621 aaggaagtat agtatcatca ttaccagagt agatagcaaa gtcatctgga actaaactag
    34681 ctatttctgc aacctgtcct aggtctccac tagcttcttt tacagcaact atgttatcaa
    34741 ttttagcaag ttcaactata aggctaggac ttatatttac acaagttctt ccagggacat
    34801 tgtaaagtat aattggaagt ttaacactgt tagctattgt ttcaaaatga cgcttaagtc
    34861 cagctttatt agctttattg taataaggtg ttattattaa tagagcatct actcctaatt
    34921 tttcagcttc ttgacttaag ttaactgagt gcatagtatc attagaacca cttccagcta
    34981 taacaggaac tcttttatta actttttcaa caacatactt aatagtagca agttgttctt
    35041 catcagtcat agtattagct tcaccagtag taccacaaga tactatagca tcagtaccat
    35101 tagctatttg gtattcaact aattcaccta atttgtcaaa atcaactttg ttatcctttg
    35161 taaatggtgt aactagagca acagcagaac ctttaaatat catataaaat caatcccctc
    35221 ttttttattt ttgtgtttta tattacagtt acttctaatg cttgtttttg agttactatt
    35281 aatattttac aaaattatat atttatacaa gtttgtatat tatactaaat cgtatgataa
    35341 agccatttca gctatttgaa ttgcgttagt tgcagcacct tttcttatat tatcagcaac
    35401 aacccaaaga tttacaccat tatcaacact aaaatctctt cttattcttc caacaaatac
    35461 ttcatctcta tcatcaaatt ctacagcagt tggatattcg tttttagcag gattatcaac
    35521 tactacaaca cctggagctt cttcaagtgt ttttactaaa tcatctaact caaatggatt
    35581 gttaaactca acatttatag attcactatg accatttttt actggaactc ttacagtagt
    35641 tgcagttatt tttaaatcgt agttatcaag tattttcata gtttcattta ccattttcat
    35701 ttcttcttta gtgtatccat tttcagtgaa tgaatctata tgaggaagac agttgtaaga
    35761 gatttgatga gggtagaatc cagtaggatt accgttcatt cctttctcta aatcttcaac
    35821 acctttaaca ccagaaccag atacagcttg gtaagtagag taaacaactc tcttaatttt
    35881 gtatttatca tcaagagctt taaggggaac catagcttgt atagtagagc agttaggatt
    35941 agctattata cccttatgat tttttatggc ttctggatta acctcaggaa ctactaaagg
    36001 cacatctgga ttcattctcc attgactaga gttatctact actaagattc catttgaagc
    36061 agctattgga gcatactttt cactaatact tccaccagca gagaataatg ctatttgtat
    36121 gcctctatca gtaaatgaag tttcctttaa ttcttctact atatattctt taccacaaaa
    36181 cattacttta gaaccagctg atttagaaga agagaataaa aatagatttt ctattggaaa
    36241 atttctttct tctaatactt ttaaaaatgt tctacctacc attccagtag caccaactat
    36301 tgctacattt acttttttca tataaattca ccccaatttt aaattattaa aacattattt
    36361 tattttagtt atacaagtaa gatatgttaa tatttttata tatccaaaaa ctttaatatt
    36421 atattaacaa ctttaattat atacaaaaaa cgtacaaagg tatactttgt acgtctaatt
    36481 tatcactcaa agtaaaccct gtagctcatc acctacaaaa ataagtgaca gtattacaca
    36541 tgttatgtgc aatcccagaa aatattattg caagaaatat tttcttcggc tgtaaatcct
    36601 ttttccaatc attaacacct gccagctact attggatact cttagttaca gctacctcta
    36661 ccctaggtta ataggatatt aaaatttgat tattctaata ataatacata tttaagaaaa
    36721 aatcaactta tttatataaa tattgctaaa atttaaatcc aaaatttgtc aattcttgtt
    36781 ttaacacatt aagattagtt tcttccattt cagctagagg aagtcttaaa tcaccaacat
    36841 taaaacctaa aatgttcatg gcagttttaa caggaactgg atttacttct ataaataagg
    36901 cagcaattaa agcatccata tcaagttgta atttttttga gccttcaata tcaccttcaa
    36961 agaatttagt tactaaatca tgtgtttctt taggacatat gttagcaagt acagagataa
    37021 caccacttcc acctaatgat aatagtggta gtatagtatc atcattaccg gagtatatag
    37081 caaagtcttt aggtactaat ttagctattt ctgcaacttg agctaagtct ccacttgctt
    37141 ccttcacagc aactatatta tctattttag caagttcggc cacaacagat ggctttatat
    37201 tcactttagt tcttcctggt acgttgtata aaataattgg caacttaacg ctatttgcta
    37261 ttgtttcaaa gtgaagttta agacctttct cattagtctt attatagtaa ggagttatta
    37321 ttaaaagacc atccacccct aatttttcag cttcttgact catatgaaca gacaacatag
    37381 tattgtttga accagtacct gctataacag gtattctctt attaactttt tcaactgtat
    37441 attttattac agcaaaaatt tcagattcac tcatagtagt cgcctcacct gttgttccac
    37501 aaacaactag tgcatctgtg ccattctcaa tgtgatattc gattaattca cctaattttt
    37561 caaagttgac attattatcc tctgtaaatg gggtaactaa agcaacagca gaacctttaa
    37621 ataacatata atcaaatctc cttttttatc taaatttaat tttttaagta caataataat
    37681 tatagtacaa attaattaat tttaataggt atagttaata tcttaactgt ttaaataaga
    37741 tatttatatt atattaatat tagaaaaatt ttaaataaat gttttattct caaaatatgc
    37801 atattaattt aataaaatat accaaaatgg aaataaaaaa taaattttat gagaatttaa
    37861 taactaataa ttaatatatt taggttataa taaatatgtg cgtaaaatct aacaataagc
    37921 aataataccg taaatttagg aggaataata tggatacttt aaaggaactt tttaaaatag
    37981 gaagtggacc ttcaagttct catactatgg gaccacaaag agcagcagag agatttaaga
    38041 atgaaaaccc agatgcagaa agttttagag caatattata tggtagtttg gcagctacag
    38101 ggaaaggtca cttaactgac tatataattg aaaaaactat agcacctaaa aaagtcgaaa
    38161 ttgtttggga agaagatatt ataaaagatt ttcacccaaa tgggatgaag tttgaagctt
    38221 tagataaaga taaaaatgta acagctgaat ggactgtata ttctgtaggt ggaggaacta
    38281 tagcagaaga aggtcaaaga aacagtaaaa gcaatagtat atatcactta gatactatgg
    38341 atgagatagt aaaatggtgc aaagaaaata ataagacttt ggtagatttt gtgttggaat
    38401 gtgaaccaaa agacataaaa gattatataa aaactataaa agatgctatg agaaaatcta
    38461 ttgatgatgg actaagtaca gatgaaataa taccaggcaa gttattatta aaaagaagag
    38521 caagtacatt ttataatgca tataaaaaag ataagagctt ctcaacactt gtatatgcat
    38581 atgcactagc agcttcagag caaaacgctt ctggtaatat aatagttaca gcaccaactt
    38641 gtggttctgc tggagtaata ccaggaatat tctttgcaat gcaggatttc tataattatg
    38701 atgacgaaaa aataattgaa gcactacttg tagctggaat aataggtaat ataataaaaa
    38761 ctaacgcttc aatatctgga gcagaggtag ggtgtcaagg tgaagttgga gccgcttgtt
    38821 ctatggcagc agcagcagta gcatacttaa aaggtggaac tatagaccat attgaatatg
    38881 cggcagaaat agcacttgaa catcatttag gaatgacttg tgaccctgta tatggatatg
    38941 ttcaaattcc atgtatagaa agaaatgcaa tggcagctca aagagcttat gatgcagcta
    39001 attatgcatt attaactgat ggctcacatt ctgtctcctt agaccaagtt gttgaaacaa
    39061 tgaaagaaac tggtatagat atgatggata agtacaaaga aacagctaag ggtggacttg
    39121 ctaaacattt cttctcttgc taaaaataaa attttccaat aataattcct ctgttttgat
    39181 ttatgaaaca ggggaattat tattaatggt gaattattta ataatgcaaa atatttttat
    39241 attcaattta gctttttatt tttaattagt ataacatgct atataatatt ttaattataa
    39301 agaaatatag aaatattttt taataaatta gaaattggag gagattacat gagagaggca
    39361 tcatatttac aagaacaact ttataaacat aggaagtcat taaatcctat tgcagagatt
    39421 gggagaaaag agtataagac ttcaaaatat ataagagatt atcttgataa attaggaatt
    39481 gagtatgaag tttatttaga aacaggtata gtaggtgtaa tagaaggtaa aagacctaaa
    39541 aaagatattg cttttagagc tgacatagat ggtcttcata gtgaagaagg ggtaaaacat
    39601 ttatgtggtc atgatggaca tgctactatc ttacttggat taattgagta tttaaatgat
    39661 aataaagaag acttaaatga taatatagta tttatattcc aaccggcaga agaaggtcct
    39721 gggggagcaa aagctttaat agatgaagga gtgcttagaa aatacaatat tgatgaaata
    39781 tatgggcttc atatatatcc agagttacca gaaggttatg taggtacaag agagggctat
    39841 tttatggctc aaataggaga tattgacata gaaatagtgt ctaaaagtgg acatggggct
    39901 atgccacaaa atggtattga tggtatagtg atagcttcaa actttgtaag ctcacttcaa
    39961 actatagtgt ctagaaatat aagcccaata gacaatgcag tgttgactat tggcagaatt
    40021 gaagggggag ctagaagaaa tataatagct gaaaatataa agatggaagg tactatgaga
    40081 tgtttcagcc cagaagtgta tgagaaaatg aagcttagag ttagagaact tgctagaggg
    40141 tttgaattgg cttataactg taaggttaat ataaatatta tagatgatta tatcgctgtt
    40201 aaaaatgaca aggatttgta tcaagagttt gtagaggcta ttggaagtga tactgttgta
    40261 gagttagagc cacttatgat atcagaagat ttttcttatt atcaaaaaga agttccagga
    40321 ttatttttta tgttgggttc aagaaacgaa gaaaaaggat ttgtaaatgg tcttcataat
    40381 ataaacttta attttgatga aaagatatgt gtaaatgcat tgaatgttta tttaaaatta
    40441 ttaaaataca aagaagcaat agattaggaa aataaattat aaagattaaa aagcataaat
    40501 acctaaattt aaaaaaagtg tattaataaa taccccttac atatttgtat aaatttattt
    40561 taattgacaa aaatacaaat aagaaggggt gatttttttg aaagaaaaag tacaaagaga
    40621 aaagaagata actaatggca agaatactaa aatattgaca aatgatgata taatgaaata
    40681 tgagatagcc tcagaactag gtcttatgga taaagttggt gaacgtggat gggcaggact
    40741 tacagctaag gaagcaggaa gaattggagg tatgcttaca tcacgcaaaa agaggcttaa
    40801 aaagattgtg gaggagcaga ataaaaagga tgagtcaata tagtgatttt gcgtttgtat
    40861 atgatgaact tatgaatgaa gttgattata atggttgggt taaatatata gaagatataa
    40921 tagatagtga aggtgtaaaa gtaaaaaata tattggaatt ggcatgtggt acgggtaatc
    40981 ttacaattcc attggctaaa aaaaattacg atatagctgg tatagatata tctgatgaga
    41041 tgcttagtgt agcaagagaa aaggcagaaa aagaaggagt agaattagtt ttacttcaac
    41101 aagatatatc agagcttgat tttgaaatat ctgatttgga ctgtgtactt tgtgcttgtg
    41161 atggatttaa ttatataact tatgatgatg atttggaaaa tgtattttca aagacatatg
    41221 aattattaaa gaaagatgga atattcatat ttgatataag ttctttttat aaattagcca
    41281 acatattggg aaataacatg tatggagaaa atagagaaga tatagcgtat atgtggcaaa
    41341 attactttga tgaagaagag aatctaattg aaatggaact tacattcttt attaaagatg
    41401 aatgtggaaa gtttgaaaga tttgaagaga tacaccaaca aagagcatat actgaagatg
    41461 agatagttga tatgcttgaa aaatctggat ttaaggatat aaaatattat ggggatttta
    41521 catttgacaa acctagtgaa gaaagtcaaa gaatcttctt tgtatgtaaa aaataagata
    41581 ttcttgacta tataagataa ttattaaaaa taaccatgat ataaaaatat agaaaatgca
    41641 gttttataga ttaaaagtaa agtgatgata ctttatgact tttatttttt aataattata
    41701 aagttctata gataaaaatt aaaatatagt aactattact tagtaaaatt aaaataaaat
    41761 gggttttgcc caaggttaag atagataagt atttatagat aaattaaggt agtaatataa
    41821 taaaaataag acaattaacg ggacatggag gaatattaat gagagattat gtactaagag
    41881 cgacatcagg aaatggtcaa gttagagcat ttgtcgcaac tactagaaat acagtagaag
    41941 aagctagaag attacatgaa actacaaaag tagcaacagc agcacttgga agaacattaa
    42001 cagccacatc tataatggga ttaatgatga aaaatgatag tgataaattg actgtaataa
    42061 taaaaggtgg aggtccaatt ggaactatta tcgccacatc agactctaag ggaatggtaa
    42121 aaggatatgt aggaaatcca caagtagagg ttgaagacta tccaaatgga aagttaaatg
    42181 tagcagcagc agtaggtaca gaaggtgttg taaaagttat aaaagactta ggtcttagag
    42241 aaccatataa tgggacttat ccattggtaa gtggagaaat tgcagaagac tttacttatt
    42301 attttgctgt atcagagcag actccatcag tagtagcttt aggtgttctt acaaaagaag
    42361 atgaagtaga gtttgctgga ggatttatag ttcaattaat gccagatgca gaagaagaga
    42421 ctattgctaa gttagaggaa aatgtagcta aattaccttc tataactaac atgttaaaag
    42481 aaggaaaatc accagaggat atattaaata ttgttttgga tggtttggaa cctaaaatac
    42541 ttgatacctg tgaagttggg tttatgtgtg aatgttcaaa agaaagagta aaaactgctt
    42601 tggttgcaat tggtaaaaaa tcattggctc aaataataga agaagataaa aaagcagaag
    42661 ttggttgcca gttctgtaat aagaagtata tgtatagtga agaagagttg cttgaaattt
    42721 taaaggaaat gtagactata aaaatagttg tattgatata agtaatgtta tagtaatata
    42781 gtaaagtcgt attgatatag taattaaagt ataatttatt aataatagac atatttatat
    42841 taaaaaataa gtttaataaa tttttaagct taataaattt taagaattaa gaagctttct
    42901 ggctgtaatg tttgcaatgt tgcagttaga aggctttttt attttattat agttcgtgtc
    42961 aaagattgta tgaaacaata aaatattgta aaataatttg tatatttaga cttgatttac
    43021 acaaaggttt ataagatatt tgagttgatt atacgaaatt ctatgaagta gtaaccctgt
    43081 gactttaatc atgggaggtt cagaagaagg agaaaatttt aaatatggaa agtaaaacga
    43141 agtacatagg aataagtata ttaatattat tgatgatatt tttaattgca aagaatttta
    43201 acggaaaact tgaaatacct attcctgaga gtaatagtag aaaatttaaa tcagcagcag
    43261 tgttttatcc tcaacatcaa gatgatgagg ttttatgggg aggaagtgcc attgtagatg
    43321 ctataaaaca gtgtggtgtt gataatgtat atgtggtatt agtatcagat ggttctggag
    43381 ttaacgtttt caaagctaac acaaaattta gaaatttaac taggaagcaa aaggaagaac
    43441 ttagaaataa cgagtttaaa tcagctttga gagagcttgg agtgaaacct caaaatgtga
    43501 ttatacttgc agatatagat aaaaaggaag gcatacatta tgaattaatg gaaaagacta
    43561 tattggattt tgaacataag tttaaaggca atgtgaccca tatagcccat cactataagt
    43621 atgatgacca tataatgcat agaaagaatg gggaagtttt aaagaaactt aaacgtgaag
    43681 gtaagataaa agatgacttg tattttgtta agccaaaata tgtaaagtat ataccaagaa
    43741 atgaaagagc tatctataaa gcaatggatt ttgatgacta caataaggtt aagagtgctt
    43801 gttatgaata taaggttgta aattcaaagc gtaaaatgta tggtatagga tatatttcgg
    43861 cacatagtta ttttgaacat cttctaaatg accctgaact tacgtctgta ttaagtgaag
    43921 attaatttct tataagtagt aagttatgtt tgataagttg attggaattt tctatagttt
    43981 tgaaggtatt ggaaatttag ttttatttaa agatttaaag atagaggttt ttagtgttta
    44041 aaattttaaa gttaagttga gtaattgtat agaattattt gtgaaagaag aaacagtgta
    44101 aaacatagta tcttatttat ggttttaaaa tcataggcaa gatacttttt ttatgtaact
    44161 aataccattt tgagattatt aacttaagaa ttttatataa tttaacactc atatataatg
    44221 atgatattta ggtagaatag atgcaattac gcttaaataa aataactgta ttttttataa
    44281 aaatcaaacc ctacactata ctaccagata aaatctacat gctataattt gacctgtgtt
    44341 tataaaaaaa ggagtaatct aataaaatag tatttcactt atgtataact aacaactatt
    44401 attacgattt tctctaaata atttaaagaa aaataatgca ggaggtaaaa tatgaacaat
    44461 gaatttataa ttttaaaagg ttgcaaagag aacaatttga acaatatttc cttaaaaata
    44521 ccaaaaagaa aaataaccat attcacaggg gtgtcgggtt ctggaaagtc atctattgta
    44581 tttgagacaa ttgcaaaaga atcacaacgc caactaaatg aaaggtttag tacatttgta
    44641 agaagttttt tacctaagta tggagaggtt aaagctgatt gtatagaaaa tttgtcaaca
    44701 ccaattatca tagaccaaag tagacttggt ggaaattcaa ggtcaaccct aggtactatt
    44761 acagatgtaa attccttttt aagagcttta tattcaaggt ttggtagtga gtatataggt
    44821 aatgccaata tgttttcttt taataatatt gatggtatgt gcccacactg tcatgggctt
    44881 ggaaaaaagc tagttcctaa tatgaaccaa attcttgata tgaataaatc attaaatgaa
    44941 ggtgctatat tgctttcagg gtttggagtg ggttcttgga attggaatat attcgctgaa
    45001 tcaggatttt ttgatattaa taagaggata tgtgattatt cagaagtaga attggaaaag
    45061 tttttgcatg gtaaagcaga aaagattaag atagaaaatg ctggtcaaac taacatgaca
    45121 tatgagggac taatggttaa atttaatcgc ctatacttaa gtagagaggg agaaatatct
    45181 gaagctacta agaaaaaatt gagtaaactt cttatagaag atagatgtcc tatatgtgat
    45241 ggaagaagac ttaatgaaag agtatatagg tctttaatta atggttataa tatagcagat
    45301 ttaactagta tgcaaattga tgaattggct gaagttatta aaagtataga tgaggcagaa
    45361 gcacaacctt taattaaagg aataatagaa aagttaaaca gtatcataga tataggtctt
    45421 ggatatttaa cactggatag agagacatct tctctatcag gaggggaatc tcagcgtata
    45481 aagatggtta agtatttaaa tagtaatctt gtagatttaa tgtatatttt tgatgaacca
    45541 agtgtaggac ttcatccaaa agatgtgtat aaattaaata atcttttaaa aaaattgcgt
    45601 gataaaggga atacaattat agttgttgaa catgacccag atgttattaa gattgcagac
    45661 catattgtag atgtaggacc aaaggcaggt aagtatggtg gagaagttgt gtatgaaggc
    45721 agttatgaaa atctacttac aagtggtact ttgacaggga atgctttaag taaatttctt
    45781 cctattaaag aaagtgtaag agaacatagt ggatatttag aagttaaaaa ttgtaataaa
    45841 aacaatttaa aaaatgtatc gattaagata cctaaaggtg tattgacttt agttactgga
    45901 gtagcaggag caggtaaaag tacgttgatt aaggatgaat ttttaaagca aaatcctagt
    45961 gctgtactta tagaccaaag ccctgtttca gcaaattcaa ggtcgagttt ggcaacatat
    46021 agtggaatta tgaacaacat aagaagcata ttttcaaaaa caaatggcgt aaatgcttct
    46081 ttattcagtt caaattcaga aggagcttgt gataactgta agggttctgg aatagtagaa
    46141 atgaatcttg cttttatgga gagtataaag agtacctgca atgtttgtga aggtaaaaaa
    46201 tttaaaaaag aagttttaga atataggttt caagggaaga atataattga agttcttgaa
    46261 atgtctgttt tagacgcaat tgagtttttt aatttaaaac agattaaaac gaaattacaa
    46321 tgtattgaaa aaatgggtat tggatacctt actttaggac aaactcttga tactttgtct
    46381 ggaggagagt gtcaaagatt aaagcttgct agtgaattaa ataaggaaag ttcagtgtat
    46441 atattagatg aaccaacaac aggtcttcat atggctgata ttgagaattt tataagtata
    46501 attgaagaga ttgtagataa tggaaatacg gtcataataa ttgaacacaa tatagatgta
    46561 attaaaagag ctgattggat tattgagctg ggacctgaag gaggaacaaa aggcgggaga
    46621 gtaatatttg aaggtattcc aaaacaatta tgtaacaata aaatatcatt aacagcaaaa
    46681 tatatagtat aataaataaa atataataaa attataatga tattttaata aatataatgg
    46741 gtatataaaa tgataattaa acttaacagt aataaataat gtgagaaata ttcaaattta
    46801 attaaataaa gtgtcagaaa tttgtttaaa ttttagtatt ttaacggttc tgaccttttt
    46861 tcttattttt atggagaata tcgaggatgg agtctctaat agttactttt ttgtgtctac
    46921 ttgcaagttt gattaaaaaa tggctataca cacttgtgac ttcagtcatg ggaggttcag
    46981 attataatct taatatataa tttgtgaaat aatttgatga cgatttgctg atgtataaac
    47041 aataaagatt aactacatta aaatttaaat cttgttaagt aaaacttata aaaggagaga
    47101 gatggatgat aaatgatatt ctaaccttat tatctgaaag aagtgatttt tttataaagc
    47161 tatccataga acatttgatg atttctatta tttctataat aattgcgatt gttttgggtg
    47221 gtataattgg tattattgta agtgaatttc ataattcttc taaacctata ttgggaacta
    47281 tcaattttct atacaccatt ccatctattt ctatgctagg atttctgata ccattttccg
    47341 gaattggaaa tactactgct gttattgcac ttaccattta tgcattattg ccaatggtta
    47401 gaaatacata tactggaatt actaatgtgg ataaaaatat tcttgaagca gctaggggaa
    47461 tgggaagtac agattttcag atattgtata aaataaaact tccgttagcc ctacctgtta
    47521 ttatgtctgg tattagaaat atggtgatta tgacaattgc attagctggt atcgcttcat
    47581 ttataggtgc tggaggccta ggtgtagcca tatatagagg aatcacgaca aataacacaa
    47641 taatgacaat tactggaagt atacttattg ctctattagc tttaatattt gattttattt
    47701 tagggctggt agaaaaattt tttcaacgaa aaagaaaaaa gaaaaatagg aaaaaagttt
    47761 ttgccattct aggaagcctt attcttatag taattatctt tacgtcttat ttatttaatc
    47821 cttcaaagac tcaaactatt catattgcta ctaaacctat gactgaacaa tatattcttg
    47881 gtgagatgtt ggatatttta attgaaaaaa acacaggtct aaacgtggaa attacacaag
    47941 gtgtaggagg gggaacatct aatatccagc cagctatgga gaatggagaa tttgacattt
    48001 atccagagta tacaggtaca ggctggaata tggtacttaa gaaaaatgaa ttatatactg
    48061 aaagtatgtt tagtaaacta cagtcggaat acaaaactaa acttaaaatg caatggactg
    48121 gtatgtatgg tttttctaat agctatgcat tagcagttag aaaagaagtt gcagataaat
    48181 acaatttaaa aaatataacg gacttaaaag atgtatcttc taaattgaca tttggagctg
    48241 aatatgactt ttatgagaga gaggatggat atactccatt gtgtaataca tatggactga
    48301 actttaaaga tacagttgat ttagatattg gattaaaata ccaagctatc aatgaaggta
    48361 agattgacgt aatgcctata tttacaactg atggtcagta tagtgtttca gatgttgtag
    48421 ttctaaaaga tgataaaaat ttctacccat cttatcaatg tggaaatgtt gttcgtttgg
    48481 aagtattaga ggaaaatcca gaactatcaa aggtatttaa gatggttgga gatattttga
    48541 ctgataaaga aatggctaaa atgaattatg acgtagaaaa caacaattta gaacctagag
    48601 aagttgctcg tgagtttctg aataagaaag gtcttttata aattggagga aatataatga
    48661 cacctattat acaatttaaa aatattaaaa aacaatataa tgataaaact ataatagata
    48721 atcttaattt agatatagaa aaaggagagt ttttgactgt aattggttcg tctggttctg
    48781 gtaagacaac actcttaaaa atgattaatg gtctgatttt gccagatgga ggaaatattt
    48841 tgataaacca aacagatatc aaaaacgagg acttaattaa attaagaaga agaataggat
    48901 attgtgtaca gggaagtgtt ctttttcctc atatgactgt tgaagaaaat atttcatatg
    48961 taccaaatct cctaagtaaa aagaataaat tagaagtaaa aagtgctgta aacaagtgga
    49021 tggaaatagt gggattgcca aatgatatga aagtgcgtta cccatcagaa ttgtcaggtg
    49081 gtcagcaaca acgtgtaggg attgcaagag ctttagcttc ttctccagaa attttattaa
    49141 tggatgaacc ttttggtgca gttgatgaga ttacacgaaa gcaattacaa aaagagataa
    49201 aagaaattca taaaaaaaca ggagttacga ttatctttat tacgcatgat atttatgaag
    49261 ctttgatttt gggaacaaag actttggtat taaatcatgg agttgttcaa cagtatgata
    49321 ctcctgagaa tattttaaat actcctgcta accaatttgt agaccagtta ttgaatataa
    49381 gaaaatctat aatagatgat gataatttaa aagaataaat ttaaatttaa aatcaaattg
    49441 ttgtgataaa aactattgat ttagagctaa cttcaagttg tatacttgaa gtggaaaagt
    49501 tagattaagg tatagaaggg catttagcag ttggctgtag acgaggtaac tgctgaaggt
    49561 gctttcaata aatggctaga acctgtatct gttgaagatt ataataaatt gaaataagaa
    49621 gctcaatata cagagtagat aggcacttca aatagaagtg tcttttgtgt gccataaaat
    49681 aaatcaaaat attagcaaag aatgtgaaaa acaaaataaa ataatatgct atctttaaaa
    49741 taggagggat gctatatgga taacaatttg attaccattg aaaaaacttt agattatata
    49801 gaaaaacatc ttttagatga gatgttagat ttaaatgtaa tttctaaaga gataggctat
    49861 tcaaaatacc atttacatag aatgtttaca tcagttgtcg gatttagtgt acataattat
    49921 atacaaagac gtagacttac tgaagcagcg agaatgctaa tttttacaga aaaaccaatt
    49981 attgagattg ctttaatctc ggggtatgat acacaacgct ctttttcaag agggtttaaa
    50041 aaaatgttta aacgtagtcc taattttttt agaagacatc aagatttttt accactacaa
    50101 ttaaagtttg atattcataa tagaaaaaaa ttacgtggag atatggtttt agatgttaaa
    50161 aaagttaatt gtgcagatat aaatattgtt ggctattctg gtaaagtaag aaaaggattt
    50221 catattattg gaaaatgctg gagggatttg cataaaaata aagagatgat agaaaataag
    50281 gtagatttag gttttttaat tggagttagt gattatagca attttaaaga gattgagaat
    50341 actcctacat ttacatacat tgcaggtgct caagtagcta cttttggcaa attacctaat
    50401 ggaatggaaa aattcgtatt accgaaaagt aattatattg tttttacttt tattgggaaa
    50461 aatgaggata gtatgcaaaa tgttgtagaa tatatctatc aagaatggtt ccctaattca
    50521 agttgccagt ttaatgaaaa taatttatat gactttgtaa aatatggaga aaaaactgat
    50581 gagaatggtt tgagtgaaat tcagttttgg gttccaatca tttaaaagaa ggtattatgt
    50641 ataaagtaga tggatttata taggcaaagt tttacagcag tatgtattta taaataattt
    50701 atagtttgta ggaaagaaat aattttacta taagattatg ataaaataca caatactatg
    50761 acaaaatagt gttaaataat agatttaatt ttgcatagga aaagttctat aatggtgtgt
    50821 attcataaat aatttataat ctgtaggaaa gaaatagttt tactataaga ttatgataaa
    50881 atacacaata ctatgataaa atagtactaa ataatagatt taattttgca aaggataagg
    50941 tgttttaata aaaatgaatg tagaaaacga attacttaaa aacctagata gattgcatac
    51001 aacagagtta ggcgttgtaa gaattaagaa aaatctttct ttggagacaa atgatgtagt
    51061 gaactggtgt aaaactaaaa tagaatctcc aaatgccatt attaatagaa agggaaaaaa
    51121 ctggtatata agtgtatatg attgtattat aacagtaaac gctcacagtt atacaattat
    51181 tacagctcat aaagaaaaga aatgaattgc aaaatacaaa ggtcaaaagc attttccact
    51241 gtttgtagta cctatgaaga agtatataaa ataatattac ttttgaataa taatttaaat
    51301 tttggaggtt gaaaaaatga atgatactgt aaaagaaaaa ttggcagagt cactaacttc
    51361 tgatactact gatattttac cttatttgcc ttatttatta caagattttt gggaattagg
    51421 aagttcacca gaacaaatgt tacaattatt tagaaagcat atacctttta atgaggatac
    51481 aaaagttttg gatttagctt gtggaaaagg tgctgtatct gtgaagcttg caaaagagtt
    51541 gaatatacat atcaaaggta ttgatttgat acctgaattt attgattatg ctagaagaaa
    51601 ggctattgaa tataaggttg atgctttgtg tgattttgaa atgaatgatg ctaatagttc
    51661 tgtaaaaatt gagcatgatt atgattgtgt gatttttgga gctgtagggg atattttagg
    51721 taattataaa gaaactataa gtaagctatt acctacaata aaaagtaagg ggttcatttt
    51781 aattgatgat gcatatgtag tagataaatc tacaaatgaa cagcttaatt ttgagagggt
    51841 gtatcccact tacaatgagt ggtgtatatt gttcaaagaa ttaggtctga agctaattga
    51901 atgtatacca tgtttagaac ttgaaaatga tgttgaagag gaaatgaatt ggattataac
    51961 tcgtgcaaat gagctaatta aaaaatatcc ttctcaaaaa gatatgtttg aacaatatgt
    52021 tattagccaa aaaaatgaat atactgattt acagcaggac cttgttggag caacttggat
    52081 tttacaaaaa gaataagata aatattagac tagataatca atgttatagt gaattatccc
    52141 atgtctagtg aatataaata acataaaaaa ccgtcaagat gaatagttta ttcgtgcttg
    52201 acggttttta gtatttgttg aagtaaaatt ttatttcttt agtatatcac ttttagtcaa
    52261 tccatttgat ttagcttgag tattccattg atgattcccc ataagtttat ataggaatct
    52321 agggaagtta ggtgaaataa aaatagtact tttgttggaa tcatctatat gtatattatc
    52381 actaagtata ttttttgaaa gttcttttaa tgcaattcct aaatcttttt taggtccttt
    52441 acccaaaggt acggattcta gtgaaccaaa catctcccca gctccaattc caataccttg
    52501 aacccatgta aagttgattt ttttacacca atgctctatc atttgaattg ccaaatgatt
    52561 atgttcacct tcattgaatc cacagttaac tattgcatac actggaatag gttttttatt
    52621 ttgtgttttt aaaaattttt cacatttctc taaaaaatga agtaaatgag atggaatacc
    52681 atctacataa agaggagatg cgattattaa tctatcacaa ttaagtatgg cattaagagc
    52741 gtcaggatac ttagatgtgc tatctacttt aaatgtaaaa actttattat cattttgaat
    52801 aagttgtttt aattcagata aaaaatactg tgaattattt ttatttactt ttggactacc
    52861 atttatcatt actatattca tatggattca cctcttatat cttgtatatt ttcatagaaa
    52921 caaacagagt gttcttttat attgaaattt atagcatttc catttactaa tttcgtagca
    52981 gtttcttttt cagcttctgt aatacaatct ccatagaata tagcttttaa atttatctgt
    53041 ttatcatatc tgcttttatg acgcatcata ttatctttta ttacaaagta tggaagaata
    53101 taattaatgc ttttatcaag tacgtttttg acaaaagggc taaaccctcc gtaacagcat
    53161 ctgcttatta taataattcc atcagagtca tttataagtt tacccatgtt gctgtaataa
    53221 tcttttacaa cacattcaga aggagtttta atccagcaac caaaacaacc aatacatttg
    53281 tggatttctt ttgtgtttgg aagtattttt atattgtcat caagtttggg taataacttt
    53341 tctaattctt tgtcagttaa atcatgtatt aaaagcctca taaagtccat ctccttttat
    53401 tatgtatgtt tacttctaaa aattatatct tatagatttt tcagtaaatc tttacaccaa
    53461 ttaataacaa aattataata gtcaactcca taaatcattg ttgcatacat atatgtatat
    53521 tggtcaacac aatgattttc ttctgcatca tttttttgtt tacttaattt atttagaagt
    53581 aattctactt cagttattaa catttttaag tttgtttcaa ttatttcttt gggtagatac
    53641 ttgtaaaaga atatattaac aaggtggtct tgctttgttt tagaaaaatt tataggtttc
    53701 tttaaccatt caagaaatac ttctttacca gtattagtta tggaatatag tttttttaat
    53761 ttgctaccat ctataacttc gtgatagtgt atatatcctt ttgtttctaa tcttttaagt
    53821 gcaggataga tacttccaaa gctggcgtca aaaaagtacg aggtactttc tgacattatt
    53881 tgttttaagt cgtatccact taattctttt tccattaaaa atcctaaaat aatgtattct
    53941 aacatattac ctcctggtat caattagata tatatcgatt cgatatattt attattacag
    54001 ataataaatc ttacgtcaag atgcttttta aaataagcta aaaagaatta ggagttattg
    54061 ataaatatgc aaaaaaatta atataaaagg taattaaata tcaatgaaga tttaaaaata
    54121 gttaaatcaa agtaagagtc taatatataa aatttatagt aactttatgg tttaaatata
    54181 aacttgtgtg ctattgacaa agtataaaat tagcagtaaa ataattgcat gagcaaatag
    54241 attgttgcga acgcaaaaaa ataaagacaa taaatattaa aggagagaat atgagagagc
    54301 tattaaaatg ggtttccatt ataaatagat acaatacaaa ttatattgac aggaaattgg
    54361 cggatattgg aatcaataat agccaatatt tttatgtatt acacatatgt aataatccag
    54421 gtattacaca ggacactata tttgagaaaa ttcttgtgaa tcctagtaat ataacaagag
    54481 ctttggcaaa tttagaaaaa gaaggcttta taacaagaga accaggtgta aaggataaaa
    54541 gaacatggca tttatatcct acagaaaaat ctttagaatg ttatgatgaa atactaaaaa
    54601 taacaaatgg gtatgttgag gagttgttga gttcatttga gaaagaagag caagagttgt
    54661 ttatgtcaat gttaaagaaa gttgcatttt tggctattga tatgaatgaa gaagcaaaat
    54721 tggaggcgaa ggaaaatgga agaaaaatat aaggagacat tggaatatga aaaggtttca
    54781 aagttattaa ctaagtacgc aattcctagt attatagcaa tgttagtaag tgctttatat
    54841 aacattgttg accaaatatt tattgggcaa ggtgttggtg tattgggtaa tgctgctaca
    54901 aatgtcactt ttccactaac aacaatatgt actgcaattg cattattgtt aggaattgga
    54961 ggagcgtcta agtgttcatt ggaattaggt gctaaaaatt cagaaaaagc tacaaaagca
    55021 gcaggaaatt ctatatgctt gatggctgtt tttggaataa gtttatttat agtagtatca
    55081 atatttttaa caccactatt aaaattcttt ggctctacat cagaaatact accttatgca
    55141 cacacatata caaaaattac atcaattgga ttaccatttc ttataatgtc cactggaatg
    55201 agtaagttga tacttgcaga tgggagaccg aaagcttcta tgttttgtat gttaatagga
    55261 gcagttatta atacaatttt gaatccatta ttcatttttg tttttaatat gggaattgct
    55321 gggtctgctt tagcaactgt tattgggcaa cttatatctt ttggaataag tgttcattat
    55381 ataaagaatt ttcaacatat taaattacat aagaaaagtt ttaaattaga tagtataatt
    55441 tataaacata tatttgcgct aggttcaagc tcatgtttta atcaattagc aatgactgta
    55501 acacagatag ttatgaataa tacattggca tattatgggg ctaggtcagt ttatggaagt
    55561 gagattccac ttgcttgtgt cggtatcatt attaaagtaa atatgatttt tatatctata
    55621 attattggta tatcacaagg tatgcaacca attatagggt ataattatgg ggctgaaaag
    55681 tataatcgtg taaaagaagc atataagttt gcagtgggaa gtgcaactat tatatctgtt
    55741 attgcattct tatgtttcca attcttccca agacaaatta cgtctatatt tggtaacgga
    55801 aatgagactt attttatatt tgcagaacat tattttagaa ctttcttgtt cttaacattt
    55861 ataaatggtg ttcaaatagt atcatcaact ttctttacat caattggtaa atcagtgttg
    55921 ggacttttta cttctttgac tcgtcaaatt ttatttttaa ttccattgat agtaatttta
    55981 ccaatgttta tgggaattga tggagtaatg tttgcaggac caatagcaga tgcagcagcg
    56041 gcaatggtat gtatatactt tgcagttcat gaaatgaaat cattgactat aaagcaaaat
    56101 aatttgatga gtaaccataa taagattgca gaggtataag acaatattta agaagtatag
    56161 aaaattaatt taaagaagta taataaacta atttaggaag tatagaaaat taatctaaag
    56221 aagtataata aactaattta gaaagtatag aaaattaatc taaagaagtg taataaacta
    56281 atttaggaag tatagaaaat taatctaaag aagtgtaata aactaattta ggaagtatag
    56341 aaaattaatt taaagaagta taataaacta atttaggaag tatagaaagc taatctaaag
    56401 aagtataata aactaattta ggaagtatag aaaattaatc taaagaagta taataaacta
    56461 atttaggaag tatagaaaat taatttaaag aaatacaata cattaatttt taagtaacat
    56521 taaattaatg taagtaaaat aatgttcata tgaagattta aaggtgcatt ttctgaagct
    56581 aagtatatgt tgtagagtgt tatgtctctc tagcctgtga agtttaatag ctcaagaaaa
    56641 tgcactattt atgatgatta ttttgtttta tttccaattt tggaaataac tattctaatt
    56701 aaatctaaaa ttataccata aataagaaaa gaaagtatag atgctatgta ccaataaatc
    56761 gaaattgacc ctgtaattgt tctacatggg atttgcatac ctgaatccat aaaaacaaaa
    56821 aaactatcaa ctctggaaat aatagacaaa ataggattta aacctattag aacaatactt
    56881 tttgagtctt gaccaatttg atgcatataa atctcaaata atgcaattaa aaaaaaccaa
    56941 aatgtaaaag atttaataat ttttttcatc aaaatacctc acataatata aattttgttt
    57001 gcactatttg cttactacaa ctatttatct ccattcagtc acatcactta attgtaatct
    57061 aggtcttctt gatggttctt catcaggata acccaaagat attgctgcca ctaattgact
    57121 atctgtattt aaccattcac ataattcacg atatgcaaag tatacatcac aaatccaaag
    57181 actacctaga cctaattcca cagctgtaag cgacatattc tgaattgcag ctccaattga
    57241 ttgcatgttt gccatttcat agaatcgttc ttcaggggat agcttttcta aaggtgattt
    57301 acctaatata tttaaaataa aaattgttac tggagcttgt ttcatgattt caactgtgta
    57361 atttgcacca gcgatatgtt gacgacttcc tggaagaagc ccattatcat taatttcatt
    57421 ttgaatacct ttgctcatgg ctttaagcat agattctttt tccttttcag tgatgacaac
    57481 aaaacgccat ggttgcctgt ttttagatga aggtgcattt attccagctt caattatttt
    57541 ctctatagtt tcatgtgaaa tgctttgatt tttatatttt ctgatacttc gtctttttga
    57601 tatagaatca cttatcattt taagtaacct ccgtttattt tttggaaaaa tctatttaat
    57661 ttttattttc tattttaatt attttagcat taatcagaaa aaaagtcatt gattttagta
    57721 tacaattatt aatgattata ttaagaattg tatcaaaaag tatgtgatta tactaaggga
    57781 ttatattaaa tattatattt aaaattgtat gaaaggttgt atttaatatt atattgagag
    57841 agatatatat taggatttat atatattcag atttattatt atattgagcc ttatattaaa
    57901 ttaaggacta taatttgaaa ataagctata aaaactcaat attgttttgt tcagagataa
    57961 aaaaagaata ctttaggtga tttttattaa aaattaagag tgataagatt atatacttta
    58021 taaatgtaaa tatgacgaat aaaaatgata aatataataa tatatatatt tacattagga
    58081 gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt taggacttaa
    58141 aattgtctaa aaataaatat aagtgatgaa gaggtgatgt gatgagatta gatatggata
    58201 gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga agtatatttt
    58261 atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga gacatatttg
    58321 taaatagaat tacatattct gaagaacggt tgcctaaaca gtatatagtt tttcagaaat
    58381 atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta taaattctaa
    58441 agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat tgcaaggatt
    58501 attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac ttataacttt
    58561 aattgttaaa ttagtactta tgccactatc attaaaacaa agattttctg taaaaaaaca
    58621 gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg atactaaaga
    58681 attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc tgggatgtct
    58741 tatagttttg gctcaaatgc cagtagtata tgctttatat catgtatgtt tagacatgac
    58801 aaaggaattt acaacaatta taattccgtg gattgctaat ttaggtacat ttgataattt
    58861 gtttatatta ccttgtatat atacattaat tatgttagct tcaaatttag taaattatat
    58921 accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg tggtgactac
    58981 cataacaagt cttatactaa ctgttagaac tccagtagcc ataggtctgt actttataac
    59041 aagtgcaatt tattcattaa ttgaagatat ttgttttaga atatatttta atcgaaaaaa
    59101 tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc taacatagaa
    59161 aaagttagta ggctattttt atctatgata tcaattccat taagaagagt ctaaaaaata
    59221 ctatcaaaat gctataaaaa atactatcaa aatactataa aaaaatagtt gaaaaataaa
    59281 taaattaata taaactaaaa taaagcctac aaaaaagagg tgataaatag aaatggctaa
    59341 aatactaatt gtagaagatg aaaaaaggat gcaaagtatt attgtagaat atatgcaaaa
    59401 gggaggatat acttgtataa ctgctgatga tggagtcgag gcacttacaa tattaaagag
    59461 caacaatata gatttaatga tattggatat tatgatgccg tatttagatg gattttcagt
    59521 gtgtagagtt tcacgtgaaa tgactaatat acctattatt atattgacag ctaaaggtga
    59581 agaggaggac aaattaaagg gttatgaata tggcgcagat gattatataa caaaaccatt
    59641 tagcccaaaa gtacttttag caaaagttaa tgctttgtta cgtagataca ctactgatat
    59701 tcccaaaaat tcattgagtt taggaaagat ttttattatg gtagcatcaa gacaagtgta
    59761 tgtagaagat aaattgattg atttaacata taaagaattt gaattattgc gtttatttat
    59821 ggaaaaccct aatcaagttt tttccagaga gaaactttta aattgtatat ggggatatga
    59881 ttttgaagga aacacacgaa ctgtagatac acatataaaa accttacgca aaaagcttgg
    59941 tagtgaagga caccatatag ttacattaat tcgttcagga tataagtttg aggttaaggc
    60001 atgaatagtt taagaaagat tatagaaaaa ctgaaaaatg tagatatttc aaattggatt
    60061 aaacaaaatt ttggactata ttcagttaga aaaaaagtgt ttttactttc taaattatca
    60121 ggagcgttaa ttatattatt ttatctggtt gttgaagaat tttcgataaa ctctaaattt
    60181 gaattttgga tttggtttat aatactggtt atatttatta tgagtattga tttcttattg
    60241 ggaaaattta tttcagaacc aatcacatct ataaataaat ctgcaaaaag tatgtcccaa
    60301 cttgattttt ctaatccatg tactgtaaat actaatgatg aatttggaga actttctagg
    60361 agcctgaata caatgtctac aaatctacaa caggcattat cagacctaga aagtgcaaat
    60421 attcaacttg aaaaggatgt aaataaggaa agaatgctat tggaacaaag aaaggagttg
    60481 gtagatacaa tttcacatga aatgaagact ccacttggca ttatacgtgc gtatacagag
    60541 gggcttatgg atgaagttga tgaagaaaaa aagaaaaatt atatgaatgt tataatagaa
    60601 gaaactgata gaatgaacaa tatgattgta tcattattag acttatctgc attggaagca
    60661 ggagtttcta aacttaaccc agagcgtttt gattttatag agtttgttga aactgtggca
    60721 ggtcgtcttt taattgatat accagatgcc aatttttatt ttacatatga tttgccagag
    60781 tatgaagttt ttgtagtggc agataaaatg agaatggaac aagtagtaga gaatctagtt
    60841 attaatgcaa agaagcatgt tgtatataat ggtaatttag atttatcgat tacatgtgaa
    60901 aatggcttgc ttttatttga aatttataat gatggtaggt ttatagagca agatgagatt
    60961 tcaaagattt ggtctaagtt ttatcgtagt gttcaatctc agcgtacagg aggttcaggt
    61021 ctagggcttg caattgtttc acaaatactt acaatgcaag ggttagaata tggagtgaat
    61081 aatcgtgata gaggagtaga gttttatttt atgattccaa ttgatgaata atttagtatt
    61141 aatttataaa tcataagtat ctgatgtaaa atatttttat ttgtatatgt aagcatttaa
    61201 agagaataaa ataaaaaaac ttaatgaaaa aagcttaatg aaaaagtcta ataaaaagtt
    61261 taataaaaag cttaataaaa agtcttgttt tcctgatgaa aacaagattt tttattaagc
    61321 tttttattat ttcacttcga tttcacaaga gagacactct gatttcacca aatagaaata
    61381 aaataggtta aataaattaa aaaatagaag cgtaattaaa taaggttaca agggggagta
    61441 cttatgaaat ggtatacatg gttgactatt atattgataa tattgctttc tattctgctt
    61501 aaaattaaat ttataaaatg gtgggataga cgacaaaaaa atgataaagg aggttatgat
    61561 agatgataaa agtaagagag ttaaaatttt cttatggaaa agataaacag atattgcatg
    61621 gacttaattt tgaagtaaaa gaaggagaaa tctttggatt ccttggacct aatgaaccat
    61681 agatggatag ataataaaaa ataaaatcta taatcacatt aaaaagtcat ttgtatatac
    61741 agatgacttt tttagtgaaa taaaagaatt tatgtatagt atatattcaa tacttatata
    61801 taatatatct tctaaagaaa gaataatctt ttatgaagaa aaagtagagt atttaaaaag
    61861 acttttgaaa tattgaacaa tagaattatt tttattaata taatgatgga aaaaatttag
    61921 ggaaagatgt ataataaagg aaaatttcag aattaaaaac ataagatatg gaattgggtt
    61981 tgggggagga aaaccattat gcaattaaca aaaataattc ttagtaacta taggtgtttt
    62041 ggaaaagaac cgacaactat tgaaatcaat aatttaactg gattcatagg tcataatagt
    62101 agtggtaaaa ctgctttatt aagtgcactt ataaaacttt tcggagaaaa atctagtgat
    62161 agaattattg aaagatcaga tttccatata cctgaaagca ctgatccgga tagtgttgat
    62221 atgaatgaat tatatattga aacacaattt gaatttcctg aattggagag ggatgaagat
    62281 aacttatcga taccagtatt ttttaaaaac atggttgtta acgaaccggg aggaaaacct
    62341 tatttaagga ttaggcttga ggcaagttgg gaaaaaagtt caaatcctga agggagtatt
    62401 gacacgaata tatactatat aacgtctaca acagaggata taaatcctga agataagcgt
    62461 aatattaaaa gacatgatct tgatcagata aagattattt atgtacctgc cattagaaag
    62521 ccagatgaac aattaagaaa tgtatcaggg acaataattt caagattact gaatggtata
    62581 agttggtcag aacaaactaa aacaaaaatt aaaacgaaag caaatgaaac tgagaaaatt
    62641 ttctttgaag aaaaaggggt cgaatcttta aagaaatcat tagaaactca atggaatagt
    62701 tatcataaag atattagata ctcaaatcca aagataaact ttaatagtac tgatttagaa
    62761 acaattttaa agaaagtgga agtaactttt tcacctacag agacacatcg ggactatcgt
    62821 gttgaagaac ttggggatgg tttacgttct ttattttatc tttcgcttgt aaatacattt
    62881 ttagaaattg aagaagaaat tctaaaatct attaatgatg acaaagaaga agagatattt
    62941 acattaatac cacctgtgtt aacgattgtt gcagttgaag agccagaaaa tcatatttca
    63001 cctcatcttt taggaagagt agttgaaaaa ttgaatctta tttctaaaaa agaaaattca
    63061 caaacaatat tgacatcaca ttcaccagct atagtgaaaa gaattgaacc aacagatata
    63121 agatatttta gaatttgtaa ggaacatgaa tgtacaaaag tgaagtcaat tatattacca
    63181 aataaagaaa aggaagtaga acaatttaaa tttgtaaagg aagcagtatt agcatatcct
    63241 gaactttatt ttgcaaaact tgttgtattg ggtgaaggtg acagtgaaga gattattatt
    63301 aagaagatga ttgaattaaa agcagaatct gtagattcat cagaaatttc aatagtacca
    63361 ttaggtggta ggcatgttaa tcatttttgg cgtttattaa atgatttaga aattccattt
    63421 attacactat tagatttaga tagagaacga gatggaggtg gttggggacg tataaagtat
    63481 gcaatacaac aattgataaa gattggtcgt gataagaatg tattacttga tacaaaggat
    63541 ggaattttac aagataatga atttaaagat atgcatactt ggaatgtaac taagactgaa
    63601 attatgttgg aatggattaa aatgcttaaa gagtatgatg tttatttttc atctccgtta
    63661 gatatagatt ttgcaatgtt acaaagtttt aaggataaat atattgcaac attagaaagt
    63721 agtcaagggc ctataataaa agggtatggg aaaatacaaa atcttgaaat taatgaagat
    63781 actgaagagg gactgaaaaa tgcttatcaa gctagaatag ttagcgatat taaatcaaca
    63841 ttaaaacaag aaggtggcga tggatctact tatactgaag aagagaaaaa acttatgatc
    63901 tggtattcat atttcttttt aggaagaggt aaacccacta ctcatttatc tttattttca
    63961 aataatactg atttagattt tgaaagtttt ccagaatgtc taaaggagtt tgtaaataga
    64021 gttattgaaa agatgataga tgatttaggg gatgaagaag ataatgaaga ggaattggat
    64081 tgttaaggaa gagtggcatc cagcatcagg atttaaatta gaagttgagg cgatgaatac
    64141 agttattaat gataaaaata cacttgttgt agctggtcct ggagcgggaa aaacagaatt
    64201 attagcacag agagcttgct ttttgttaga gactaataca tgtaaatatc ctaaaaaaat
    64261 ccttgctatt agttttaaaa aggatgctgc tgataattta aaagagcgtg tagaattaag
    64321 atgtggcaaa gaattagctt taagatttga atcaaagaca tatgatgctt ttgcaaaaga
    64381 aatcgtagat agatttagaa atagtttaga tgaaaaatat agacctttaa ataattacaa
    64441 tatagctaca aatggtgatg ttagaagagc atttgagata tcaggattaa atttcagagg
    64501 aaatcaaact gaatttaata aattatatgg aaataaatta tcgataaata aattgccttt
    64561 acatacaaat tttgtagata agatttttat agatgcttgg aatgtactac ttaaaggtaa
    64621 agaagataat aattttgaaa gttcacttac ttttgaaatg atatcacgtt tagcagaata
    64681 tttgataaga accaataggt atattaaaaa agcaattgaa atgacttata gtcatgtgtt
    64741 tttagatgag tttcaagata ctacatctat tcaatatgat cttgttaaag cctgtttttt
    64801 taaaacagaa acaataatta ctgctgttgg tgatggtaaa caacgaatta tgttatgggc
    64861 tggtgctaga aaatcaatat ttgaagattt tcaaaatgat tttttaagtc aaaaaagaac
    64921 attaattatg aaccatcgtt cagcgccaag acttgtagag attcaaaaag caatgtataa
    64981 cagattcaat gaagatgcaa ttgatttaaa aactaatgaa aagtggaatc atgaagatgg
    65041 agaagcttat ttaagacttt ttgaggatta tataaaagaa gctgatatac ttagtgaaga
    65101 aatagtcgga ttggtagaaa gcggaataaa aattaatgaa atatgtatat tagtaaagca
    65161 gagcgtagat atttattcaa aagagattat acataaactt tctgaatgta atattaaggc
    65221 acgtaatgaa gcagtatatc aggaactatt aaaagaagat ataataaaga tacttgtttc
    65281 gctttttagg ttgtcattaa atgacagaag ccctgatgat tgggattata tttacgatat
    65341 tctaggtgaa ttatatggaa tagatgaagg ttataatcca aaagtattaa atgattttat
    65401 taccaaagtg gaattaatga ttaatgatat caaagtaaat ttaaatatta ctgatgaaaa
    65461 acagttctca gatttagttg attctaaggt tgagtatatt tcatacgaaa aatttgcatc
    65521 aaagtttcaa caatataaat ctaaagatta ttttgatgat ttggtagata aatttaaaaa
    65581 gctagtatgg agtgaatata atgaaactaa ggattggatt aaagcgattg ataactttga
    65641 agggaaaaat tcaattccaa taatgacaat tcataaaagc aaaggacttg aatatgatac
    65701 tatctttttt gttggtttag aagactcagc attttggaat tttaaaaatc aaccagatga
    65761 agatagatgt gcattttttg ttgcattatc aagagctaaa agaagaattg attttacatt
    65821 tagtttagat agaccagcag gattttcgaa ttcgcagacg catgattgta ttaatgaatt
    65881 ctacgaatta tttgatgata ctaagattgt aaaaaggata gagtattgtg attaagatta
    65941 tgaaaaattt agtatattat attttaagat attagttata tgagtagaca ttgaatttat
    66001 atattcgatg tctatttttt atataaaaat atattgaaga aaggatgata gttaatgaag
    66061 aaaaagttaa aagcatctaa aagaatttta agttttatta tggtttttat aactataatt
    66121 actactatac ttacatcatc ggtttcagtt tatgctagtg agttggatat gagtgaacat
    66181 acaggattta attatttagg tattagtcca attacaggaa gaaaaattaa tcataatatt
    66241 tatcgtatga aaatggataa taaagttgta ttttgtgtag aaccaggaat taaaacagca
    66301 agtggaggcg gatatatatc ggaggagtat ataaattcaa agaaagatat attatctaag
    66361 atagcttact atggatatac agatacaaat aaatcaaaat atgactatgc attaactcaa
    66421 ataattatat gggaggagtt aggagataaa tttatatcta caacacttcc caattattat
    66481 aaaagaaaag ctgaaatact tgaaaaggta aataagcata atatattacc ttcatggaac
    66541 aatgaaaaag ttgcagttaa aagtggagaa acaattgagt taaaagataa gaatggcatt
    66601 attagagaaa tgaaactaga atcaaataat acgggaacaa atatagtact tgaagaaaat
    66661 actttaaaaa ttacagcaaa tgaaaaatcg acttctggag ttattagcta tagaaaaatt
    66721 ccagaaaatg aggttggaac atcaatagtc tatcgtaagc ctaactatca aagcttagca
    66781 gagtttcata tggcaagttc aatgagtgca attttaaata taaatgtaat aaagttagga
    66841 aacattaaag caaaaaaggt tgatgaagat acaggaaagg cattagcaaa tactaagctt
    66901 aaatttgagt atgatggtaa gggcaaagaa attataacgg attctaatgg aattgcgaat
    66961 atagaagata taccggaagg aacaactgta acaatatcag aagtgatagc accaaatgga
    67021 tattttaata aaggtgaaat taaaaaagtt gttattgaag caaataaaac tatagaagtt
    67081 acattaggaa ataaagagca gttaggtaaa gtaataatta gtaagagtgg aaaagatttt
    67141 ggaatagaaa tgcctaatga tagttataaa ttagaaggag caatttatgg aatttataat
    67201 gaaaatgatc aaaagataac tactataacc acagataaat tcgggaaggc aacatcagaa
    67261 aatataaagc ttggaaagta ttatgcactt gaagaaaagg ctccagaggg atatttaata
    67321 agtaaagaaa aaataccatt tgaattaaaa tatgctggac aaactgtaga agtaacatca
    67381 acatctatta gtcaaaaaga tacagaacaa aaaggaaaag caatattaat taaagaagat
    67441 gaaaaaactg gatcaatacc tcaaggacgt gcaaaacttg atggtgcagt ttatgaatta
    67501 agaaaatcaa ataatgatga gttagttgaa acagtaacga ttaaaggggg aaaagcaaaa
    67561 gtagaaaatt tatatcttgg taagtattat tggattgaaa agaaagctcc agaaggatat
    67621 ttattagata ctactaaaca ttattttgaa ataagttacg caggtgaaaa tgtagaaaca
    67681 gctataaaag aaacactagt aaaagagaaa gtaattacag gtggatttga tttaataaag
    67741 tttggagaat atgattggaa agaaaaaata aaaaatattt tcaaaacaaa aaataaagaa
    67801 ataaaatctt taaaagatgt agagttttct gtatttagtg atacaacagg aaagttagtt
    67861 aaaacaggat atacagatga aaaagggtat ctaaaatttg aaagattacc atatgatact
    67921 tacacagtaa aagaaactaa aacaccagaa ggatatattg cagcaaaaga ttttaaagta
    67981 acagttaaaa aacaaaatga aacacatcat tatgcaatac agaatagagt aattgaagaa
    68041 aaattgaaag ttgtaaagat agattcagta actaagaaga taataccagt aaagggagct
    68101 gaatttaaaa taaaaagctt acaaactgga gattttataa caatgcctaa aaataatgaa
    68161 gatgaaaaaa ctgatatgtt ctatacaaat gatgaaggat atttaataac accagaagca
    68221 ttgtcatacg gagaatatga attaacagaa gttaaagctc cggatggtta tgtattagca
    68281 aaagatccta tacattttaa agtagatggt tcatcaaatg ggattattga aataagattt
    68341 gagaatacat cacaaaaagg tattgttgaa tttacaaaga aaggacaaac acctaaagat
    68401 ataaaaatag aagaaatgaa atatgggaaa ttacatgaga taatatatga atataaacct
    68461 ttagaaaatg tggcctatga cattatagca aaagaaaata ttactacaaa tgatggaact
    68521 atacacttca agaaaggcga tgttgtagaa acacttacaa ctgatgaaaa aggaaaatgg
    68581 aaatcatcag aattgtatct aggaaaatat caagctattg aaaagagtgc tccaaatgga
    68641 tacataatat cctcaaatcc gatagaattt gaattaagtt attcaggtca gctagttgaa
    68701 ttaactaaaa ctagtcttac agcaaataat gatttgcaat ctttattagt taatatattt
    68761 aaaaatgaag aaaaaattga aggatggaaa gataacaaac ctgtaataaa agatattaaa
    68821 ggtaatggta aaatatttgg aatatttact agggaaaaac aagtgatatc tgataatatt
    68881 aagattccaa aagattcaat ggtaggaatt aaagaggtta aagatggagt tgctgaatta
    68941 aatataaaac taccagaagg taaatattat ttaaaagaaa tagattcagg agaaaatcat
    69001 attttaaatg agaaagaata tgattttgaa tttaactcaa ccaataatga gaaggaaata
    69061 aaagttaata tttatgaaga tggtgtagac tttggagata agaatgaaaa tataaaacca
    69121 atattaaata aacttcaatt aaatagcttt aaaataaaaa agattaatga aaaagctata
    69181 aagaataaaa gaaatggatt tagctttgag tttactgaat taggtcaagg atctaagttt
    69241 atattagaag ataaaaaagg agatataata caagaattaa caataggaaa aggctcaata
    69301 ggcgaattta acaatatacc agtaggaaca ttttatttaa aagaaaaaga aacatctaat
    69361 gataagtata tattatctaa taaagtaata cgtattgaat caacaaaaga agggataaaa
    69421 gcctttgatg aagataataa tttaatttca gaaaaaaata ataatgaaga taagatatta
    69481 cttgaaatta aaaatgaatt gattaaagga ggagctaagt taatcaagac tgatttagta
    69541 acaggggaag ctttacctaa tacaggaatt agaatattaa atgaaaataa aaatacttta
    69601 atagaaggta aaacagatga taaaggtgta ttttattttg ataatttacc taagggaata
    69661 tattacttcc aagagtatga agcacctaaa ggttatcaaa ttgatgaaac accaatgaaa
    69721 tttgaaataa aggaaaatgg tgaagttgta acttgcaaaa tgactaataa gaaaatcgag
    69781 aaagtttcta tacctaaaac aggagattac aatagcatta ttttatatgg aatagtattg
    69841 ctagcatcag gaacattatt attaaaaatg agacgtaaaa aagataaata gaagtatgtg
    69901 aatgtcagat acaaataagt atctgttttt ttatacaatt ttttagggga ggtaccatga
    69961 attttaaagt agtgtttatt agatcaccga cacttaaaag tattaattta aagaattata
    70021 taattttaga aaagagaggt aaatgattat gaaattaaaa catattattc ctaacatgga
    70081 gaagacattt ggaaatttag aatatgctgg agaaggcgat gttgaacaaa aaagaataaa
    70141 cggtaaaatg acagttatat ctagatcata taatctttat tctaatatac agagagcaga
    70201 tgatatagtt gttgttttac cagctaatgc aggtgaaaaa tgctttgaag ttgaagaaag
    70261 ggtaaagtta ataaacccta aaattatagc agaaggatat aaaataggaa ctcgtggttt
    70321 tacaaattat attttattag cagatgatat agtaaaggct taaggaggat gagattatga
    70381 gattagcaaa tggaatagtt atagataaag aaaaaacatt tggaatgtta aagttttcag
    70441 cattaagaag agaagtacgt gttcaaaatg atgatgggac gccatcaaat gaaattaaag
    70501 aacgtactta tgatttaaag agtaggggac aaggaagaat gatacaagtt agtattccag
    70561 catcaatacc tttaaaagaa tttgattata atgcagaagt agatatcatt aatccaattg
    70621 cagatacagt agctacagct aattttaatg gtactgatgt agattggtat attaaagctg
    70681 atgatatagt attaaaaaat aaaaaaacta gtaagtaaaa atcaaaaata atacattaat
    70741 aagtttactt atatagtata aagagatata atatttttac aaataatggt ctaatgttta
    70801 tactatttag tattagaaat aaggggatgg ggaattgtga atcaatttaa atatgaagag
    70861 ctagaaagcc gttatatata ctatatgaaa aactataatc ctatttttaa ttatgttcat
    70921 gatatgggag aaactcaggt tattattgat cctattgata aggtttgtag attttgtggt
    70981 aaaaaatacc cagaagtgaa attcaagaaa aaagcacatg ctatatctga aatgcttggt
    71041 aataaagaat ttgtattacg aaacgagtgt gattcttgta atatcttttt tggtcagaag
    71101 ttagaagata acttagggaa atacttaggt cttggtagaa ccttatcaca aatatatggg
    71161 aaagaaggag ttccatctta taaatcaaga gataggaaaa gtagagtaga ttttacggat
    71221 aaaggtatag ttattcaagc aactatagat agcaatttta tagagcaata tgataattat
    71281 atagtatttc actcaatacg cgatacctat actccaattg ctgtttataa ggctttagta
    71341 aaaatggcat tatcacttat tccatttgaa caaatgtcac tgtttaagga tactgttgag
    71401 tggttaaaag aggagaatca tgttatctct aaacataata tggataatta tacttatgtg
    71461 atagagagat atgttccagg tccaaagcct atgcaattaa gagctatggg atttgttaga
    71521 aaatatgatg ttcttttaat gccttattat cagtttatat tagaatttgc aaactatagt
    71581 tatcagatta ttgtaccatg taaaaaaaag gataaagaat tatgtggaca tgaaattgat
    71641 tttataccaa tacctggaac acatgaagtg atgtgcacag ctacagatag agttttaacg
    71701 gaattaaagt atatgggcaa taaagaaaaa gtaaaagatg aacctttaga tatttgcatg
    71761 aactttgaaa aatgtgaatt atacgaaggg aatggaaaaa gtataaatga aatattaaaa
    71821 gaagaaggaa ttgatttgag caagagatta aaataaataa ttaagtattc aatctaattt
    71881 aatgttcaat atgaaggagg gaaataagtg cttaagtatc cattatcagt agttttagat
    71941 acaaatatat ttataggatg tagatatgat ttaaatgaag aaggtttact tataaagctt
    72001 aaaaacttgg tgaataataa taaggttagg atttatataa gtaacattgt tttaagagaa
    72061 actgagagac atataaaagc agatatttcg aaggcaatac aggagttaaa acaagctaga
    72121 aggaacatat caaaaaatat ttctccgact attgtaaata atacatcact tgagaatata
    72181 tttggattgc catgccaaaa tacaatagaa aaaactgcta ttactagatt tcgagaattt
    72241 ttagatgagt ctaaggtaat ctatcttgat aatgaaggta tagatattga tgagattcta
    72301 gaggattatt ttaaaggtaa tgctccattt gaaaataatg aagcaaaaaa atatgaattt
    72361 cctgatgcat ttattatatc aaagttaaaa aaagaatttc ataaaaatag acctgtatgg
    72421 gtagttagcg cagatagagg attcagaaag gcattagaca gtagagatgg tttcaactgc
    72481 ttagcttcaa ttaatgagct tttggatatg ataaataaac aagatcaaaa aatgtatgat
    72541 acgattgtag aatatattga aaataaagat gcatataaag aaatatgtaa taaaatagaa
    72601 gaaaaaatag aatctgatga tattgaagtt aatggactag attatgatcg taaaggatgc
    72661 tgtggaggtt atgaatatat cgatacttat attacggatg tttccgttga aaagttttat
    72721 cttagttctg tagatgaaat tagtaatgac atagttaact tgacaatatt ctgcaaagca
    72781 aaaattgatg tgtcttgttt ctacaatgac tatgataact cgatttggga ttctgaggaa
    72841 aaagaatata tgtttttatt aaatgaagaa gttgatgaag aacatgagcc agaatttgaa
    72901 tgtagattaa gtctgaaggt taatcattat ggtgataaag ttgaattttc attatctgat
    72961 atttcatatg atttggtttt ggatcaaagt tcaaggacaa aacagagttt agttgaacca
    73021 gaagatccta gaatagcagc agaagcagat atgatggatg ctttggagga gtattacaac
    73081 cattagcaag taggataact ataatagatt ttcattaata tttgaaacag tagatttgta
    73141 aaatttactg ttttttattt ttactaaaaa ggagagaaaa tgaaatttta tagatgtaga
    73201 ggaaagcgaa taagagctag cgataaatca ctgttttttc gctttttaac aagtatatta
    73261 tcagctatat ttatcatatc attgatactt tttaatggga aacagatcgt acaaataaat
    73321 tggaagaata taagcttggt tttagagaat ggtttattat ttttatctat attttgtaat
    73381 atattaacga tagtattatc tattagtatt tgtatagtta ctactttttt atattattac
    73441 tttaaacgag atagaataac gcaacttatt cataggcaaa aactagctag aatgatatta
    73501 gaaaacaaat ggtatgaatc agagtctatt aaagatgacg gattttttaa agacttaaat
    73561 tctaataaac agaaagaaaa aatagtatat ttccctaaaa tgtattatga attaaaggag
    73621 gggttaattt atgttaaagt tgaaataact ttaggcaagt atcaggataa gttattaaat
    73681 ttagagaaga agttagaatc aggtttatat tgtgagttgg tttctaaaga gttaaaagat
    73741 tcttatattg agtatgtttt actgtatgat actattgcaa atagaatatc cattgatgaa
    73801 attaaagttc gtaatggaag tttaaaactt atgaagtctg tatattggga gtttgataaa
    73861 ttaccacaca tgctaattgc aggaggaact ggagggggaa agacatattt tatacttaca
    73921 attattgaag cattactaag tacagactct atactatatg ttttagatcc taaaaatgcg
    73981 gatctcgctg atttaagaac agttatgcca aatgtttatt ataaaaaaga tgatatgatt
    74041 tcatgcataa acaattttta tgatgaaatg atgaagcgta gtgaaactat gaaagctatg
    74101 agtaattata aaactggaga aaactatgct tatcttggac tgccagctaa ctttttaata
    74161 tttgatgaat atgttgcctt tatggaaatg ttaggaacta aagaaaatac ttctgtttta
    74221 aataaattaa agcaaatagt aatgttaggt agacagtctg gattttttct tatattggct
    74281 tgccaacgtc cagatgcgaa gtatcttggt gatggtattc gtgatcaatt caattttaga
    74341 gtagcacttg gaaggatgtc tgagcttgga tataatatga tgtttgggga atcaaataaa
    74401 gagtttttct taaaacaaat taagggtagg ggatatgtag atgtaggaac aaatgttata
    74461 agtgaatttt ataccccgtt agtaccaaaa ggtcatgatt ttttgaaaga aatagataaa
    74521 attattaaga gcaggcaggt agaacaagcg gcgtgcgaag cgaaagccga tggttcgaac
    74581 tagcctgcta ttggtatggc tttgccatac caatatttat tagcccccgg tatctaacag
    74641 gggggcacaa atcgactaaa atcatttaag caaaacttat aaacgtaagg tagaaacaat
    74701 gttataagaa attaaaggta tgtgaaaaaa tttaatgtta tttcacatac cttttttaat
    74761 aggaggataa gcaattgagt atagaagcat ggattaaaaa tttaagaaat aaaaggattg
    74821 aatatggtat ttctcaaaac aaactagcat tatcagtagg tattacaaga gagtacttaa
    74881 ataaaataga aagtgggaaa ataataataa aaaatgatat gaaagagaag ttaattaaat
    74941 caatagaaag atttaatcca gatttgccac taacaatgat gattgattat ataagaataa
    75001 gatttccaac aacagatgta aaaaatgttg ttaatgatat attgaaatta aaacttgaat
    75061 atatgataca tgaagattat ggattttact cttactcaga gcattacgtt ataggcaata
    75121 tatttgtttt agtatctcaa gatatagaaa aaggagtttt aatagaattg aaaggacaag
    75181 ggtgtagaca atttgaaaac ttcttattag cacaacatag atcatggtat gactttttat
    75241 tagaggcatt attagctgga ggcgttatga aaagaatgga ccttgctatt aatgatatga
    75301 caggaatact agacataaaa gaattaacaa ataaatgtaa taatgaagaa tgtatttctg
    75361 tatttcgtag tttcaaatcg tatagatcag gtgaattagt tagaagagat gaaaaaatag
    75421 gtatgggaaa tactttatat attggttcat taaaaagtga ggtttatttt tgtatttatg
    75481 aaaaagatta tgagcagtat ataaaactgg atatacctat tgatgaagca aaaataaaaa
    75541 atagatttga aataagattg aaaaatgaaa gagcatatca tgcagcagtt gatttattag
    75601 caaatagaga tgctgaaaga acagcatttt caattataaa tagatatata cgttttgtag
    75661 ataaacagaa agataaaagt aggagtgaat ggaagattaa tgaacattgg aactatttta
    75721 taggtgaagg cagagaaaaa ttaaaattaa caacaagtcc agaaccgtac aatattcata
    75781 aaactttaaa ctggctatcg agacaagttg caccaacttt aaaaactata aaaagattag
    75841 atatattaaa taacacaaca ttaattgaag atttaataaa atatgcaaac ttaacagatc
    75901 gtcataaaaa aataattgaa caaaattcgg tgaacattga agatattata tatgatgagt
    75961 aaaagaaagt gtagttggtt caatagataa agcaatatag atttaataaa aaagaaggga
    76021 gatttgagaa atgaattttg gtcaaaattt atataactgg tttttatcaa atgcacaatc
    76081 attagtatta atggctatag ttgtaattgg tgtatatcta ggattcaaaa gagagttttc
    76141 aaaacttata ggatttttag ttgttgcatt aattgcagta ggtttagtgt ttaatgcagc
    76201 tggagttaaa gatatattac ttaagttatt taaccaaatt atcggtgttt aaaattggaa
    76261 aatattacat taactatgat ataatatgtc cataaaacga ataaggagga cattatatga
    76321 gggagagtgt taaaaatttt ctagttaaaa aatataatga attggattac aaatcttctt
    76381 gcacttgttt aaaatatcac tataaatata atggtgtaaa tgtaaatata tattttgatg
    76441 catatgattt aaatagttta tctttatatc ttgttttaat ttatgaacaa aaatattatt
    76501 atactccact taatatttca aatactaata tacataatga ataccttaaa gaaattccac
    76561 aggaaatatt aaatcaaata ttagaaaata atcatttagt tgaattctat agttatatgg
    76621 aggaatatat taaaaataat aatcctatac cgaattcata taaaaaagat aaaatattta
    76681 taaatacatt gaaatattca aaagacaaaa cagatttgcc tttttggtat catattcgta
    76741 gagtaaggat gtcagatgaa acattaaaaa aattaagtgc taaagcagat atttctataa
    76801 aaactttaaa atcaatacag aataaaaact taactcttgt tagaacggca gatgtagcta
    76861 aaagaaaaga gttaaccttt atattaggag gagaaggtat aacaatttaa aattagaaat
    76921 tataaatttt gcagtaaatc ttatatagat ttgctgcttt tttattatct aaaataaaaa
    76981 aggatgtgaa ataatattat ggaagagatg agagtatata ttgcaaactt agggaaatac
    77041 aatgaaggtg aattagttgg agattggttt acaccaccaa ttgattggga tgatttgaag
    77101 gaacgtatag gattaaatag tgaatatgaa gaatatgcca tacatgattg ggagttacct
    77161 tttgaaatta gtgaatacac ttcaatagaa gaaattaatc gtttatattc tatggcagaa
    77221 gaattagcat ctacagatat gggagaagca ataagtgata ttcaaaaagc ttttttctct
    77281 agttttgaag aattatatga acataaggat gatattgttt attatccagg gtgtagtgat
    77341 atgtctgatg ttgcatatta tcttatagat gattgcaata tgttaggaga aatatctgaa
    77401 aacgtgagaa actatattaa ttatgaagca tatgcacgtg acttagagat taattatagc
    77461 tttgtagtta ctaaccatgg agtatttgag tatattaatt aaattttaat agattttata
    77521 aaagacagtt aaatatagct gtcttttata ttttaaagaa agcttatgtt ttaattaagt
    77581 gttttttgta tgagtataag tatatttagt aaatatttta gatttattag caaagtaaaa
    77641 gatgttataa aatagagtaa tttaaaaata taatgttaag tttaatttgg tatttatgga
    77701 attataataa ataattcatg tataataaaa aatatatatt attgaataaa gctagtaaaa
    77761 gtctagttag tttgatttta aaactacttg ataaatagta attttaggag gtaattttat
    77821 gaaaaaaaat gaaaatgtag aaattacaaa acaagcatat tcaattttgg aaaatgggta
    77881 tgctttgcaa aaaagttcat gctgtttaca aggttcattt tttgaaggag tttccgatga
    77941 ggattataag aaaatagact ataaattaaa agcaataagg caagattttg ctaaaatttt
    78001 agtaattatg aattccattg atagaacata ttcattatat cagaaagatg aatatgttcc
    78061 atcatatatt tctattatgg gtaaccaagc aactaatgaa attggatgtt ttattgaata
    78121 cttatatgca aagtatcgtg taatattgga gtattttcag caaatactag aaatatgtat
    78181 tccacctagg ttaagtgatc cccaaagtga acaatataat aagttgaaaa agtcacataa
    78241 aaaatataat tttctattga agtaccttgc tgaaaatatt gatgaaacaa ataatattct
    78301 gaatatggaa tggtttcaac atattagagt agaaagagat tttataatac atgatggtgc
    78361 tacttgtttg gtatttggtg ataaggaaaa tttattattt aaggttatga ctactgacgc
    78421 attagataga gaatatgata ataagcaaga tgagtttttt tcaaatgaaa agggattgat
    78481 atactataat cgttattggg gagtgcaaat ttctaagtta attgtttttt ccgaaactat
    78541 atttagattt ttaataagca tcagcaatat gtcggaagaa aataaattta tattagaaga
    78601 tttatttcct caagaaagaa ataaatttat tgatagttat ggtacagaat acaatgatgt
    78661 acaagacgtt ttgataaata tgctaagaaa aataattgaa atttaaaata aataaggatt
    78721 ataaatatat gtattagact aataaattcc aattgagtga ttagaatttg aagaacaatc
    78781 ttaatatatt gcgaattaaa agtggacaat tagtttgttc acttttttta tgttcaatat
    78841 caacattagt ttaaaacaaa gcccaaataa agaaggagga atatgcttga aaaagattaa
    78901 gtcatataca ggtatttgga atgtagagaa ggttatatat gcaataaatg attttcaact
    78961 tccatttcca gttactttta cacaaatgac atggtttgta ttaacactat ttataatcat
    79021 attatttggt aagttacctc cattatatat gattgaagga gtatttttga agtatcttgg
    79081 aataccagtt tttataactt ggtttatgtc tcaaaagact tttgatggaa agaaaccata
    79141 tggattttta aagtcaataa tactatattc actaagatct aaaataacat actcaggaaa
    79201 aagtattaaa tttaaaaata aaattattga tgaaaatata actatagtaa ggagtgaatt
    79261 atatgttccc gataaaatat atagaaaata atcttgtatt taataaggag ggagaatgtt
    79321 ttgcttatta tgaattaata ccatataact attcattctt atcatcagaa caaaaattta
    79381 ttgtacacga taattttagg caattaatag cacaaaatag aaatggaaaa attcatgcac
    79441 ttcaaatagc cactgaaagt tctataagat ctacacaaga aaggtcaaag aaaaatatta
    79501 caggaagatt aaaagaaatt gcttatgaaa aaatagatga gcaaacagaa gcacttgttt
    79561 ctatgattgg cgataatcaa attgattatc gtttttttat tggctttaaa cttttagtta
    79621 cagaagaaga aatgaatata aagaatataa aaaagtcaat attagctaat ttatcagatt
    79681 ttatatatga tgttaataat aaattgatgg gtgactttat cagtataaat aatgatgaaa
    79741 ttatgagatt ttcaaaaatg gaaaagttat tagaaaataa aatttcaaga agattcaaag
    79801 ttagaaggtt agataaaaat gattttggct atttaattga acataattat ggaaagaaag
    79861 gtacagtttt tgaagattac aaatatcatt tacctacaaa gaaacttaaa agagaaacat
    79921 tagtaaagaa atacgatctt ataaagccaa ctagatgtct tatagaagag aagcaaaaat
    79981 acttaaaaat tatacatgag gattcagaaa gttttgtagc ttattttact attgaaagta
    80041 ttattggaga aatggatttt ccatctagtg agatattcta ttatcagcaa cagcagttta
    80101 catttcctat tgatactagt atgaatgttg aaattgtaac aaataaaaaa gctttaacta
    80161 cagttagaaa taagaaaaaa gaattaaaag atttagataa tcatgcttgg caggccggaa
    80221 atgaaacagg aacaaatata gtagaagcat tagatgctgt aaatgagctt gaaaataatt
    80281 tagatcaaac aaaagaatca atgtataagt taagttatgt aattagagta tcagcagata
    80341 gcattgatga attaaaacgt cgttgtgatg aggttaagga tttttatgat gatctaaatg
    80401 taaaggtagt acgtcccatg ggagatatga taggatttca taatgaattt ataccttcaa
    80461 gtaaaagata tataaatgat tatattcaat atgtaactag tgatttttta gcaggattgg
    80521 gctttggggc aactcaacaa cttggagaaa atgaaggaat atatattgga tatagtttag
    80581 ataccggaag aaacgtatat ctgaaaccat ctcttgcaag ccaaggagta aaaggttctg
    80641 taactaatgc attggcagca gcatttttag gttcattagg agggggaaag tcatttgcta
    80701 ataatttaat tgtttactat gcagtattgt ttggaggaca agctgttttg gtagatccaa
    80761 aagctgaacg tggtaattgg aaggaagacc taccagagat agctcatgaa ataaacattg
    80821 ttaatttgac aagtgatata gaaaataaag gaatgttaga tccatatgta attatgaaag
    80881 aagtaaaaga tagtgaaagt ttagctattg atatattaac ttttcttaca ggaataagta
    80941 gtcgtgatgg agagaagttt cctatactta gaaaagcaat cagaaatgtt actcagagta
    81001 aatgtagagg acttttaaaa gtaatagatg agttaaggaa agataattct caaatatcga
    81061 atagcattgc agatcatatt gaaagtttta cagattatga ttttgcacaa ttacttttct
    81121 cagatggaaa tattaaaggt tcaattaatc tagaaaaaca aatgaatata atacaagttg
    81181 aggatcttgt tttaccggat aaagatactt cttttgaaga atatacaaca atggaaagtc
    81241 tttcagtagc tatgttgata gtaattagta cctttgcatt agattttatt cattcagata
    81301 gaagtatttt taaaatagta gatttagatg aagcttggac atttcttaat gtagcacaag
    81361 gaaaaacttt atctaataaa ttagttagag caggacgtgc tatgaatgca ggagtgtatt
    81421 ttgtaactca aaatgcaggt gatgtatcag atgaaaatct aaaaaataat attggattaa
    81481 aatttgcatt tagaagtaca gatattaatg aaattaagag aacacttgaa ttttttggag
    81541 ttgataagga agatgataat aatcaaaaaa ggttaagaga gttagagaat ggacagtgtt
    81601 taatacaaga cttatatgga agagttggag taatacaaat acatccagtt tttcaagaac
    81661 ttcttactgc atttgataca agaccaccta taagaaaaga ggtggattaa tgaaatcgaa
    81721 ggtatttaga aatataagtt ggatgattat tttaatagta atacttgtat tattttttat
    81781 ctatataagt gaatctatag tttatgcaga aggactagta gatgaaactg taaattcaaa
    81841 taatgtatat tcaatgtatc ctttaagaaa ttatcagctt gatttttatg ttgataattc
    81901 ttggggatgg cttccttgga attggactga tggaataggc aaatcagtac agtatgggtt
    81961 atattgcata acaaacttta tttggataat aagtttatat ctatcaaatg caactggata
    82021 tgttgttcaa gaagcatata aattagattt tattaatgac atggcagatt caataggtaa
    82081 gagcattcaa acaattgctg gcatcaatca aaaaggaata tctaataatg gattttatat
    82141 aggttttcta cttttgttta ttttgattct tggaatttac gtagcatata taggattgat
    82201 taaaaaagaa actagtaagg caattaattc tgttattaat ttcgttgtga tatttataat
    82261 atctgcatcg tttattgctt atgctcctga gtatattaaa aaagtaaatg atttttcttc
    82321 cgatattagc acagctgctt tgaatttagg aacgaaaatt gtaacttcta attcaaatag
    82381 tgaaggaaaa gatagtataa atctaattag agataatttg ttttctatac aagtacaaca
    82441 accatggatg ttattacaat atggaaattc taacatagaa gaaataggaa aagaaagagt
    82501 agaaaaatta atttcgacag atccaggtga agatgatgga aaaaaacgag aggaaatagt
    82561 taaaactgaa atagaagaga aacataataa taatttgact ataacagaag ttatgagtag
    82621 attaggcaag gtgttttttc taattatatt taatatagga ataactattt ttatattttt
    82681 attagtatct atgatgatat tttcacagat actttttatt attttttcta tatttttgcc
    82741 attaagtttt ttactatcta tgataccctc atatgaaagc attggaagac aagcagttgt
    82801 aaagttattt aatactatca tgatgagagc tggaataact cttattataa ctgtagcatt
    82861 tagtatatca acaatgtttt ataccatatc taaagattat ccatttttta tgatttcatt
    82921 tttgcagata gtaacatttg caggtattta tatgaagtta ggcgatataa tggggatgtt
    82981 tagcttacac tcaagggata gtcaaaatat gagtagccgt atctttagaa agccatatag
    83041 aaagatgcaa aataagagta aacatataga acgttatata agacaagctt taatatctag
    83101 aggcaaagac aaaaataaac tatcatttaa cagaagtaat aaaggtaata taaataactt
    83161 acaaaaaaga gtagattatt tacctagtag agatgattat aataaatcaa ttaataaaga
    83221 aagtttctta aaacatatgg gtaataatat tggtgcagtt atagatacaa agaaaaatat
    83281 agctgataaa tcaagacaag ttaaggaaaa tataaaagat atacctatac agactaaata
    83341 tgcctttcat aatacaaaag aaaaaactaa agataatata tctgatttta aaagaggtat
    83401 cgtagaagaa aaggaaaagc gtcaacaagg tagagataaa aagcagcaaa atcataggga
    83461 aactatagct caaaaaagag ctaaattagg gaatgtagta gtatctaaag ataaaaattc
    83521 taaagataat aaaaatatag ataactactc aaatttaaaa gataaacctt caattggttc
    83581 tgaaaatgtg aaaaagaaaa aaggagaaaa tattagacca acagtttcag aaaaaatagt
    83641 taatgataag aatttagaaa aaagtaattc tataaataaa atagaaatgc ctacagaaga
    83701 aattcttcat aaaaaaagag atattacaaa tcaaggtaaa gagtcatcta ataaagttaa
    83761 aaataatcaa aatgaaaaat ttaaaaacag taaagctatt aaaatagaac gacataacat
    83821 caattctaat tctaaagatt ctaggtataa tcacataaat agtaaagaga gaaaaataaa
    83881 aaataaacct aattttactc ctgagaataa ggtgagaaaa caatgaaaat aaagatatct
    83941 attattttta ttagtatatt cttatgtatc tttggagtaa ttttactgtt tatcccttta
    84001 atatcagatg aaaattttaa tcaagataga aatcaaaata taataggaat gaacttatca
    84061 ccagaagttt taaagtataa gcctattgtt gagagatatg ctcagaaata tgatattaaa
    84121 gagtatataa attacttact tgctattatg caagttgaat caggtggaat tttagttgat
    84181 gttatgcaat cttcagaatc actaggcctt tcacctaata ccttaggaac agaagaatca
    84241 atagaacaag gatgtagata tttttcaaat cttttaaaaa cctcaaaaga aaaaggctgt
    84301 gatcttaatt caactataca agcatataac tatggatttg gatatttaaa ttatattcaa
    84361 agtaatggaa aagttcataa ttataattta gcagaaagat tttcacaaaa tatggcccag
    84421 ggagttaagg taacttatac taatccaata gctattgcta aaaatggtgg ttggagatat
    84481 aaatatggga atcaatttta tgtagaatta gtacttcagt atattggaac aagtgaattt
    84541 agtaatgaaa cagttcaaat aatcataaat gaagcattaa agtataaagg gtggaaatat
    84601 gtatttggag gagataatcc aaatacttct tttgattgta gcggtttaac acaatggtgt
    84661 tttaaaaaag caggaataaa tttaccaaga acagcacaag agcaatatga tattatgcaa
    84721 catattacac tagatcaagc aaagccagga gatctagtgt tttttcatgg gacatataat
    84781 gctggaactt atgtaactca tgttggaata tatgttggaa ataacaaaat gtatcatgca
    84841 ggagatccta ttgggtatgc agatttaacc gggagctatt ggcagaaaca tttaattgga
    84901 tcaggaagaa taaaaaatag atgaggtgaa aatatgttaa gaaaaaagga taaaaaacat
    84961 aaagtaaata agatatcagt tataagatta ggtataaata gaaagtctgt agttgtttta
    85021 tggattgttt taattgtaag tatagtgttt ggtgtttata aaaattttac tgcaatagat
    85081 aaacatacta ttcatgaaaa agagattata gaacagaaaa ttatagatac aaatggtatt
    85141 gaaaattttg tgaaaaactt tgctaaagaa tattattcat gggaaaataa caaagaatct
    85201 attgaaagaa gaacatctaa aattaatgaa tatctcataa aagaattgca ggaattaaat
    85261 agagatacaa taagaaatga cattccaaca agctctagtg taaaagatat taagatatgg
    85321 agtgtaaacc agaataaaga aaatgaactt gatgttaagt attctgtgga gcagactata
    85381 aaagagggag aagatgtaaa agatgttata tcttcatatg aagttgtagt tagaagagat
    85441 aattctggaa atatgataat tattaaaaat cctacattat ctcaaatgcc agaaaaatta
    85501 aattatgaac ctaagaaaga agaaacagat agtacaatag atcaaaaaac aattaaagaa
    85561 attacagaat ttttaaatac attctttaaa ctatatccaa atgcatctaa aaatgagtta
    85621 gaatactatg taaaagaaga tgtacttaaa cctataaata aagaatatat attttcagaa
    85681 ttagtaaatc ctattttcac aagagaagag aaaaatataa aagtatcaac tagtgtaaaa
    85741 tacatagatc aacagacaaa agcaattcaa atctctcaat acaatttaac tttagagaag
    85801 gatggtaatt ggattattag aaaataaaaa attactatat ataaataata aaaataaact
    85861 gtttagatta aaagttttgg agttcaaaac aaaaagagat aatctgtagt aatagaaaag
    85921 gagtggttaa tgccactcct aaaaagctag tttcaaacta tatggtctgc actttacacg
    85981 ctatataatt tggtctacat ttttgtacaa taaaattcta ataataattt tatattttct
    86041 aatttagtta agttaattaa gtattttttt attaaatgtt accttaatct ttaagtaaaa
    86101 cttctttttc gtcagataga agaatacttt acgatattca gataatttat agccaacaat
    86161 ataatatttc agataaagaa tattttacga tattctgata gcttatagac aacaatataa
    86221 tatctttatt tgctggtgtt tcataaactt tttgtaacaa tggtaaaatt tcgaatgcct
    86281 ttttatttaa gtctaagtcc tctataaaat ctacctttgc agttattctt aacaactcat
    86341 tattttcatt aggcatagaa caaatttgta tattattgtt ttcaactaat tgattaaaaa
    86401 aatctttttt cttagatgtt aaaaaatata attttccacc ttcaatcatt ttaaatgata
    86461 taaacctaac ctgtggctta ccattatgta tattagaaac tgcaataggt gtatttgcat
    86521 ctcctaaaaa actcattata ttctccatat tattatacct ccaactcaaa taactaataa
    86581 tttattcaat atgaaaattt aaaattttga ttgataataa aaaaagccat atcataaaaa
    86641 atatgacttt ttgagattat atagctttaa aattgtgaaa tttatgagat atacttcatc
    86701 aactgatata tatttaaatt tgttccaagt atcagagaaa tttttattat ttattttaac
    86761 tttaaatata tacattaata aaaataccta ttgctatata ttaaggatct ggttagaaaa
    86821 agatcttctt aagagtaaca tgatttcaat aaaaaaaatc ctcacattat tttttaaata
    86881 aataaaaaaa tgaatttgat tgcaatatgt aatagacctt tacagttatt agatagtgag
    86941 atagtataca cactttaaat acattcttta aacttataat ttaactttag agagatgatt
    87001 ttcattgttg tgatattttg agaatgttat aatgaaaacg taacaaaaca gtaaacatga
    87061 ttttagaatt gggggattga atgaagaaat atataccatc agttatttta ttatgtatat
    87121 ttgaaattgt ggcagttaca ttatggatta ctatggataa cttattttat atgataaatt
    87181 ttacatatat aggagtgtgt atttctatag ggcttgcgct gtttgcaaat gaaaaaaagt
    87241 atgcaagaag atttgttcaa tttgctgtgg gatcttacat gcttatttat cttggggtaa
    87301 ttcaacaaga aaatatgcag atagagggat tttggtatta tttatttctt ggagtttttg
    87361 aagcagctac catacattat actgttgcaa aaatatttgg tcctttaatt tttggtaggg
    87421 gttggtgtgg atatgcctgt tggactgtaa tgattttaga cttattaccc tataaacagc
    87481 ctaagagtcc aagaaaaaat tggggatttg ttcgatatat tacatttact ttatctttag
    87541 ttttaacaat aggattcttt ttaataaaaa ttgataattt agataaaata attttcttgc
    87601 ttttcatagt tggtaatgtc ctatattaca tagtaggaat atcctttgcc tttgttttaa
    87661 aagataatcg agctttttgc aagtatatat gtcctgttac agtattttta aaaccaatga
    87721 gttattattc tattatccgt gttcattgtg atgaagataa atgtattaag tgtggaaagt
    87781 gtttgaaagt atgtcctatg aatgtagaag tgaataagga ttcacgaaaa aggacaaatg
    87841 ctacggagtg tatcttatgc tatgaatgtg ttaaagaatg tccagtaaac gcattacgtt
    87901 aattataaaa atcctacagt atctcaaatt acagaaaaat taaatttata gaaaatgtaa
    87961 tatagaattt aaaattaata atttaaagga ttatgtttaa atctattata aatataatga
    88021 agatgtattt agtggaggta atgataaagt aatatacttt tcaattctta tatgtttaga
    88081 tctagtgatt ggcgtcgctg taaaaacata taatatcagt tctataaaag ataaatattg
    88141 tagatataaa tagagataaa atttcaatgt aatttatgaa gttatataat tataggaaaa
    88201 atagatgaag ggataaacaa agaatgattg atataaattg gaaaaaattt gaggtgaaac
    88261 atcctaaagc aactgatgca tttgaaagtt tatgctattt tttattttgt cgtaaatatg
    88321 gattaaatga ggggattcgt actgatttta atcaggtagg tcttgaaaca gagcccataa
    88381 aatatacaga tggaaagtat tatggatttc aatcaaaatt ttttgacaag aatgtatctt
    88441 atgataatat ttacgactct ataaaaaagg cgttagataa ctatgataat ttagactata
    88501 ttattatata tattaataaa gagtgtcaaa cttcgtgtaa aagtgcaaag aagatagaag
    88561 atatgtgtga ttcaagagga attaaagttg aatggtattt acctaataac tttaaattag
    88621 cgttgaatca acctaataat ttagacttag cagaatttta ttttggagca acaaatgtta
    88681 ggggattact tgctgatagt aagagtatac gtatgactac attacttcaa tctaaagagt
    88741 atttagaact taatttacat aattatgaaa aaactttaac tactacagag tactgtaagg
    88801 aagtattaag ttccaagaaa aaattacatc ttgtatctgg agcagcaggt actgggaaaa
    88861 gtgtgtgcat gcaaaaaatg ttatatattt acggaggatt tgatgaggag acaaaaaata
    88921 aacagcttga aaaagtaaag tctttgggag caatatgtat ttacataaat ttgaacaata
    88981 cagcattaga gagtttagag aatatagttt taaattataa aaatactttt tttgcagata
    89041 gtgataataa taactttata tatttatttg acggtataga tgaagttcca actagtcata
    89101 taacatctac agtattgttt attgaaaatc ttttagagaa agaaaataca aaaaaaataa
    89161 tcatttcttc aagattatca agttataata agtatatttt aaaggcttca attaatgaaa
    89221 ttgttgaata ttctattgaa aatttgaaag aggatcaagt aagagattat tttgaaaaca
    89281 aaggaaatca agagaaagtt aataaactta atgaagttgt ttctaataat gataattttt
    89341 ttgagcatgt tacagatata ttaacagtat cattactttg ggatcaaatt aataatatta
    89401 atgggaagag tttttttaca aacttaatgg atttaagtgt atcaacaata ataaatgata
    89461 tacatcacag aaaatatttg gagtctttaa atcttcctaa tccaaaggaa aaagctatta
    89521 tagaaataaa taaaaatata tctttttact tatttgaaaa agatatattt aattttactt
    89581 atgaagattt gcataaattg ataggtgaaa tatatcccag atgtgattat atttcattaa
    89641 ataaaataat agcatatata gcagaaagtt tttttgatac ttctatgaca acaggaaact
    89701 atattttttc atataagcat agacgttttt cagaatactt cacaatactc aaattagaag
    89761 ataaaattag aaatgattta aattatctta gagaaaataa cataataata aatcatgatt
    89821 tatttgaatc tatgcttatt ccgtatttgc aaataaaagc ttgtaataaa aaagatttac
    89881 ctttagcatt tcagattgga ttatttaatg tttacttagg taatgataaa gcatggggaa
    89941 tagataaaga attttacatt tggtcagatt ggattatata tgctatagct ggattagata
    90001 atgaaatatt tgagagtata atgctagaca gctcattacc aataagtaaa ttttttaacg
    90061 aagtacctaa aaaaataaaa gcattacttt cttcaaaaag taaaaaaaat ctttcttaca
    90121 atgatgaatt aagacagaat tttaaaattt atgttgtact tatttcgtta atgcataagt
    90181 ttggaaaact agaattttta tcagtattgt tacaagatta taaagaaata agaagattgt
    90241 gtaaagagaa taaattctgt ttttatagta cttcaaataa agataatttt ttagtttgga
    90301 gaagtattat atatattaat acagttattt tgaatgataa tattgatgaa ttaattagtc
    90361 tagtattaca aaattcaaaa gatacaaata ttgatggttt gcttaaggat cgtacttcaa
    90421 tgaatatatt acatttgaat tctttatatt ataatatgtt gatatatcat aaaaataaat
    90481 gtgcgaatat gatagaaaat atgaatatta atcaattaag tatttttgct attaccgcaa
    90541 caaacccaga atgtttaaat aatataatga aggataaatc attaatagaa aaaataaagc
    90601 aagtacttga agatgagatt agtaatgaaa acttaagtgg tgttatttgt ttggcattaa
    90661 agaagttttt aggaggttct ttagtaaaga aagaagaaga gcttgttaag aaatatcttg
    90721 aaaatataga atttattgga tcatcgatat tctggaaggg gcactgtgat ataagcgcct
    90781 ttatattatc tgtttttgga aaaggacaaa tcaagttaaa aattgatcaa gaaataaaga
    90841 gatatgtaaa tgtatataaa gtttttttag aattaataga taaatcatgt tcaattgaaa
    90901 gatttatatg ttgcgttcaa aagaatattt tcaacaatac gcaagattct tattatataa
    90961 gaattttgct aggaaaagta ttgtcattac ttaatgatga agattttaat ataaaaggat
    91021 cgcttgatta tttaaatgat tctattaatg atagtagaat tttaattata tattgtacaa
    91081 tgaaattata taatccatct agattcaaaa gtttattaag tataactgaa cttacgaaat
    91141 taactgaacc tagagtatat gaagatgtta attttactag tacgagtgaa tcacttttta
    91201 tgctatcatt tattttgtct aatcatgata aattactagg acatgaatta cttttaaaag
    91261 gaattagtaa tgggataatg aggatgaatg atagaaagga tacaatagga gactatcagc
    91321 tactagatag cttagagata cttctcataa ataattggat ttcaacggag aaattaatta
    91381 ttttgctaaa aagaatatta aaaattacaa acaaaatgaa tttatataat atagaaaatg
    91441 atactcatgc aagagtaatg gatattattc aaaaatatga ttttgcagct gctgaatttt
    91501 attataataa aattgcacat ttagaagagt gttataattt aatacattat agatatgcac
    91561 tatcaatgat tcagagaggc aggaatataa aatatataga agcttgtttg gacaatatta
    91621 aattatcttt tgatagatat catcaaaagg cggaatgggg aagtttttac tataaaatcg
    91681 aagtatattt aaagatatca gcaagcgatt tttattcatc atatgaacaa aagctttcat
    91741 ttgaaaaagc atgttataaa attgatgaac tagaacatgc tggttggagt agagaactta
    91801 aaaaagaaga atatgatatt tatgttgaac tatgcaaaaa atataataaa aaaattgata
    91861 ttgaaaaaga aaaagaaaat gagtattgct attcagttaa acctttagaa aaagaaaacc
    91921 aagatattct tgaaattatt aattctatta atacagaaga agatttatgt gaatttataa
    91981 aaagactaga tggagaatac tatcttgaaa acttagaaat aaatgaattg tttattaaaa
    92041 aatgtgtaga tattaatgga aatattgaag ttattattga tatgtttaca agatataatt
    92101 atccaagtag tttgtactat tcacgaaata gccataattt ctggatgaca gttctatcag
    92161 ctctaaaaaa tgcaaagtct aaaaatagta tgatgcaata tttattagag aatggcggag
    92221 gacatgatgg gtttagtgaa ttaataaaaa tttatggata tttaggaaat aaggacatat
    92281 gtattaaatt atttgaaaga atgttaacct gtgttgaatt tttattatgc taaaataaat
    92341 ttttataatt atgtcaattg acaaatattt catgtggatg gaagagggta tatcctcatt
    92401 gggaaatgat ggtatattaa ttggatatat aaagagttta atatttattg taataaagta
    92461 aaaatatgaa atattaattt aaattggagt atcgatactg taaagtatcg atgcttttat
    92521 atatttgaat aatttaagct ataaaactta aaacttgtta ttgcaagtta tataaagtgg
    92581 gctagttatg aaataactgt tatttgtaga gataatctat actccttttt tttctaaatg
    92641 gacattagta taataatatt agaggtggta gaagaaatga aaataaatga ttttatattt
    92701 agatttaagt atagaaatga tgaaaaaagt ttatgtagag taagaacatt cttaggaaga
    92761 gagaataaaa taattaactt aataacagat ttagaagatt taaatatctc aaaagtaagt
    92821 ttaattaatt ccattgaatt tgcttgttct gaattgatag aaaaaaatat gacatgtgaa
    92881 gatgctatat ttatagaaca tacagagtca gaaagtttag aagaacagaa atttgatatg
    92941 gtaaccttta atcctagagg aggacctatt tggaaaaata tcaatataaa cgaagtaaaa
    93001 aaaattttaa attgtattga tgatgaactt ataaatacaa caaaaaagaa taggaattta
    93061 gttgaaacaa tagatagaat tagatataga ataaatcctg aaactgattt tttatataaa
    93121 gaatcgccag aagtaattaa gcgaaaatta gaaattaaag aaaatagaat tagtaagagt
    93181 aaactagaag atttaataaa aaatagatca aaggagcaag aaatagctag attattgaaa
    93241 tcagatttat ctgtatttgg agaagtatat tcttttccta aagatgagta tatatgtttt
    93301 tcagagtatc caatttatga taggtatata gattatgttg ttataaccgg aaggtcaaga
    93361 atgcaagtat atttaattga aattaaagga gctgaattta atttagttaa taaagatagc
    93421 aaatttaatg agaagattaa tgaagctgac agtcaaatat ctagaacttt aggatatata
    93481 tttagaaatt atatggcgtt taaagatgat atacacgaaa aaagaagatt ggttgaaaaa
    93541 ggagagaaaa tattcaattc atttagaggg ccaattagca aattagaagt tgatagacaa
    93601 aaagatataa ctattcactc agtaataata ggtggagtaa caaatgatga tattaaagaa
    93661 agtagattaa gacatgatta tgaaaataag aatgacttca ggataaaact agaatcatgg
    93721 gatacattta taagaaaatt aagtaataga tagtttttag atattaaaaa attaattaac
    93781 tataattact attaagtttt ttatttaatt tgataggcat aaaaagaatt taatcgttaa
    93841 atatgtttag aataggtcaa tagagttaga caatgttata tgtaattagt agggaagggt
    93901 aatattagga agattagggc ccttcctctt tgatgttgtt atgattaatt atatacaatt
    93961 aaaatatata atagaagatg tttataggta attatactac tactgtctgt gaaactttat
    94021 atattgttac tatatatata acaaatt
//
LOCUS       DAMJYC010000003        92109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJYC010000003 DAMJYC010000000
VERSION     DAMJYC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 92109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.003"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..1345)
                     /gene="bclA3"
                     /locus_tag="PC472_000177"
     CDS             complement(<1..1345)
                     /gene="bclA3"
                     /locus_tag="PC472_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2725554.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGATGADGATGPTGATGAT"
     gene            complement(1412..2488)
                     /locus_tag="PC472_000178"
     CDS             complement(1412..2488)
                     /locus_tag="PC472_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2725555.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            complement(2800..3384)
                     /gene="clpP"
                     /locus_tag="PC472_000179"
     CDS             complement(2800..3384)
                     /gene="clpP"
                     /locus_tag="PC472_000179"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2725556.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            complement(3381..3839)
                     /locus_tag="PC472_000180"
     CDS             complement(3381..3839)
                     /locus_tag="PC472_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725557.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            complement(4062..5477)
                     /locus_tag="PC472_000181"
     CDS             complement(4062..5477)
                     /locus_tag="PC472_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2725558.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            5592..6260
                     /locus_tag="PC472_000182"
     CDS             5592..6260
                     /locus_tag="PC472_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2725559.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            complement(6437..6955)
                     /locus_tag="PC472_000183"
     CDS             complement(6437..6955)
                     /locus_tag="PC472_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2725560.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            7107..7430
                     /locus_tag="PC472_000184"
     CDS             7107..7430
                     /locus_tag="PC472_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725561.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            complement(7435..8019)
                     /locus_tag="PC472_000185"
     CDS             complement(7435..8019)
                     /locus_tag="PC472_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2725562.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSCVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(8068..8523)
                     /locus_tag="PC472_000186"
     CDS             complement(8068..8523)
                     /locus_tag="PC472_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2725563.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            9405..10943
                     /locus_tag="PC472_000187"
     CDS             9405..10943
                     /locus_tag="PC472_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2725564.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(11215..11928)
                     /locus_tag="PC472_000188"
     CDS             complement(11215..11928)
                     /locus_tag="PC472_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2725565.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(12376..13755)
                     /locus_tag="PC472_000189"
     CDS             complement(12376..13755)
                     /locus_tag="PC472_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2725566.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(14040..14834)
                     /locus_tag="PC472_000190"
     CDS             complement(14040..14834)
                     /locus_tag="PC472_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2725567.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYII
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(14831..15694)
                     /locus_tag="PC472_000191"
     CDS             complement(14831..15694)
                     /locus_tag="PC472_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2725568.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(15672..16667)
                     /locus_tag="PC472_000192"
     CDS             complement(15672..16667)
                     /locus_tag="PC472_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2725569.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            17371..18327
                     /locus_tag="PC472_000193"
     CDS             17371..18327
                     /locus_tag="PC472_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2725570.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(18727..19617)
                     /locus_tag="PC472_000194"
     CDS             complement(18727..19617)
                     /locus_tag="PC472_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725571.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLEKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGVSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(19795..20493)
                     /locus_tag="PC472_000195"
     CDS             complement(19795..20493)
                     /locus_tag="PC472_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725572.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(21029..22096)
                     /gene="rsgA"
                     /locus_tag="PC472_000196"
     CDS             complement(21029..22096)
                     /gene="rsgA"
                     /locus_tag="PC472_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2725573.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVYRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWKAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(22093..22494)
                     /locus_tag="PC472_000197"
     CDS             complement(22093..22494)
                     /locus_tag="PC472_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2725574.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      23290..23386
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            23440..23616
                     /locus_tag="PC472_000198"
     CDS             23440..23616
                     /locus_tag="PC472_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725575.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            24026..24664
                     /locus_tag="PC472_000199"
     CDS             24026..24664
                     /locus_tag="PC472_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725576.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQNYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(25158..26918)
                     /locus_tag="PC472_000200"
     CDS             complement(25158..26918)
                     /locus_tag="PC472_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2725577.1"
                     /translation="MQEVEVIKAKNVLSRQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSQYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKPQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(26981..27124)
                     /locus_tag="PC472_000201"
     CDS             complement(26981..27124)
                     /locus_tag="PC472_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725578.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(27443..27682)
                     /locus_tag="PC472_000202"
     CDS             complement(27443..27682)
                     /locus_tag="PC472_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2725579.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(27687..28103)
                     /locus_tag="PC472_000203"
     CDS             complement(27687..28103)
                     /locus_tag="PC472_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2725580.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGVMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(28100..28399)
                     /locus_tag="PC472_000204"
     CDS             complement(28100..28399)
                     /locus_tag="PC472_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725581.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFVVGYQIPPFTVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(29450..29617)
                     /locus_tag="PC472_000205"
     CDS             complement(29450..29617)
                     /locus_tag="PC472_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725582.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(29630..32395)
                     /gene="mgtA"
                     /locus_tag="PC472_000206"
     CDS             complement(29630..32395)
                     /gene="mgtA"
                     /locus_tag="PC472_000206"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2725583.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(32561..32872)
                     /locus_tag="PC472_000207"
     CDS             complement(32561..32872)
                     /locus_tag="PC472_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2725584.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(33119..33796)
                     /locus_tag="PC472_000208"
     CDS             complement(33119..33796)
                     /locus_tag="PC472_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2725585.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(33934..34437)
                     /locus_tag="PC472_000209"
     CDS             complement(33934..34437)
                     /locus_tag="PC472_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2725586.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(34453..37116)
                     /gene="mgtA"
                     /locus_tag="PC472_000210"
     CDS             complement(34453..37116)
                     /gene="mgtA"
                     /locus_tag="PC472_000210"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2725587.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARHSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGISTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(37209..37418)
                     /locus_tag="PC472_000211"
     CDS             complement(37209..37418)
                     /locus_tag="PC472_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725588.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSHPNETDLIELKNAISK"
     gene            37368..37704
                     /locus_tag="PC472_000212"
                     /pseudo
     CDS             37368..37704
                     /locus_tag="PC472_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37903..38805)
                     /locus_tag="PC472_000213"
     CDS             complement(37903..38805)
                     /locus_tag="PC472_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725589.1"
                     /translation="MFKKNKKSTENVKEKKVRTVKVGTHKKTVIALWVVLIESVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQAING
                     YLTKELQDLNVDTIRTDIPTSSTITDVIVWSIEQSGTDTFSATYEVDQQIEEGEQTTA
                     VKATYTVKVHVDDDGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKDSNWKIIG"
     gene            complement(38821..39828)
                     /locus_tag="PC472_000214"
     CDS             complement(38821..39828)
                     /locus_tag="PC472_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2725590.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFLGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVHVLLAIIQVESGGTAEDVMQSSESLGLLPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSVGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNKMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     E"
     gene            complement(39831..42035)
                     /locus_tag="PC472_000215"
     CDS             complement(39831..42035)
                     /locus_tag="PC472_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2725591.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIITVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRSERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARHRKTDGSATTGATRPHERPA
                     TASTVPKTSVEKMQEIKRPATAPTSKASEPVKTNVIKERPLSSGASDMKATQSAQPVH
                     RQNVEKVVSQETRQNYTKDRRTKVQQTQTVQKNQQTTEKNRNIVTKKGQKKK"
     gene            complement(42035..44485)
                     /locus_tag="PC472_000216"
     CDS             complement(42035..44485)
                     /locus_tag="PC472_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725592.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(44463..44861)
                     /locus_tag="PC472_000217"
     CDS             complement(44463..44861)
                     /locus_tag="PC472_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725593.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(44979..45482)
                     /locus_tag="PC472_000218"
     CDS             complement(44979..45482)
                     /locus_tag="PC472_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2725594.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(45500..46003)
                     /locus_tag="PC472_000219"
     CDS             complement(45500..46003)
                     /locus_tag="PC472_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2725595.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(45922..46218)
                     /locus_tag="PC472_000220"
     CDS             complement(45922..46218)
                     /locus_tag="PC472_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725596.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSYQTIKDVRKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            complement(46301..46942)
                     /locus_tag="PC472_000221"
     CDS             complement(46301..46942)
                     /locus_tag="PC472_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HDF2725597.1"
                     /translation="MEYKYTKIDLEKWNRGKLFQSYIDDMRIVMSLTVDIDVTNLIEF
                     SKSHDLKFYPAMIWVVSKVVNEHDEFKYSWDNKGNLIKWDYISPSYAEFHKEDENFTK
                     MVTDFSDSIFEFHERFMLDREKHKMERAFVANQPLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELERERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSIS"
     gene            complement(47150..47371)
                     /locus_tag="PC472_000222"
     CDS             complement(47150..47371)
                     /locus_tag="PC472_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725598.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(47372..47506)
                     /locus_tag="PC472_000223"
     CDS             complement(47372..47506)
                     /locus_tag="PC472_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2725599.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            complement(47519..48715)
                     /locus_tag="PC472_000224"
     CDS             complement(47519..48715)
                     /locus_tag="PC472_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2725600.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLNEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQATLSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(48899..50293)
                     /locus_tag="PC472_000225"
     CDS             complement(48899..50293)
                     /locus_tag="PC472_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725601.1"
                     /translation="MRKIWKKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLEKGLTLSPYNLITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNSFYEGMVQRSEEMKQHSNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLKTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(50360..50974)
                     /locus_tag="PC472_000226"
     CDS             complement(50360..50974)
                     /locus_tag="PC472_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725602.1"
                     /translation="MSEKLLDLLKIFLEKYLVPTVIAIVFSFIAYYVTPTDNKLLVKF
                     TIWGYAVFLFCVWFLIIELVIWLVQKIQYHNYSKGVEQRNKQRQAEEWQQSLEFLWTD
                     VDNLSSSDYKLLLRFIKNENQPYCSSGTPMGHCLLNSDWVHKTVSKPAKRVLIKSNQR
                     TSSKAIPIPVYETISETYQYILREDIYQMLKYSYEKYGKISHFK"
     gene            complement(51065..51448)
                     /locus_tag="PC472_000227"
     CDS             complement(51065..51448)
                     /locus_tag="PC472_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725603.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQTKK"
     gene            complement(51464..51790)
                     /locus_tag="PC472_000228"
     CDS             complement(51464..51790)
                     /locus_tag="PC472_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725604.1"
                     /translation="MEMKYVVPDMTQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(52029..55073)
                     /locus_tag="PC472_000229"
     CDS             complement(52029..55073)
                     /locus_tag="PC472_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2725605.1"
                     /translation="MKKMLKRLCTGFLALATIVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGMMKVEGETAYCIDINTDFKNGYKTRTDASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSISGATYGVFSDKDCTKQLATLTTDENGNTNVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAKFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDETTHYITKLANAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPKGYLLDGAYMQAGDKSEQIKGVYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGNFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEEMCCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNPVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LKGLEVGTKYKLVGWQMLKAENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDKPKKVTEHKDIEDEGQTITFKEKPEEPEKPETPQTPEKPN
                     RPSDSPKTGDSTNVMAFIVMLFASAGGLAGTYLYKRRKMKKS"
     gene            complement(55318..56682)
                     /gene="rlmD"
                     /locus_tag="PC472_000230"
     CDS             complement(55318..56682)
                     /gene="rlmD"
                     /locus_tag="PC472_000230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2725606.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSENLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETCVLLQ
                     RRTM"
     gene            complement(56857..58617)
                     /gene="pyk"
                     /locus_tag="PC472_000231"
     CDS             complement(56857..58617)
                     /gene="pyk"
                     /locus_tag="PC472_000231"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2725607.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(58669..59628)
                     /gene="pfkA"
                     /locus_tag="PC472_000232"
     CDS             complement(58669..59628)
                     /gene="pfkA"
                     /locus_tag="PC472_000232"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2725608.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(59837..63406)
                     /locus_tag="PC472_000233"
     CDS             complement(59837..63406)
                     /locus_tag="PC472_000233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2725609.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMNSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKVLEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(63563..64510)
                     /gene="whiA"
                     /locus_tag="PC472_000234"
     CDS             complement(63563..64510)
                     /gene="whiA"
                     /locus_tag="PC472_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2725610.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(64527..64952)
                     /locus_tag="PC472_000235"
     CDS             complement(64527..64952)
                     /locus_tag="PC472_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2725611.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(65021..66127)
                     /locus_tag="PC472_000236"
     CDS             complement(65021..66127)
                     /locus_tag="PC472_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2725612.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLKNGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVEHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(66139..66996)
                     /gene="rapZ"
                     /locus_tag="PC472_000237"
     CDS             complement(66139..66996)
                     /gene="rapZ"
                     /locus_tag="PC472_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2725613.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(67027..67740)
                     /locus_tag="PC472_000238"
     CDS             complement(67027..67740)
                     /locus_tag="PC472_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2725614.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(67744..68658)
                     /gene="murB"
                     /locus_tag="PC472_000239"
     CDS             complement(67744..68658)
                     /gene="murB"
                     /locus_tag="PC472_000239"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2725615.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(68931..69701)
                     /locus_tag="PC472_000240"
     CDS             complement(68931..69701)
                     /locus_tag="PC472_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2725616.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            70019..71500
                     /gene="cls"
                     /locus_tag="PC472_000241"
     CDS             70019..71500
                     /gene="cls"
                     /locus_tag="PC472_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2725617.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            71818..72315
                     /locus_tag="PC472_000242"
     CDS             71818..72315
                     /locus_tag="PC472_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2725618.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            72332..74218
                     /locus_tag="PC472_000243"
     CDS             72332..74218
                     /locus_tag="PC472_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2725619.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            74241..76022
                     /locus_tag="PC472_000244"
     CDS             74241..76022
                     /locus_tag="PC472_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2725620.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(76162..77958)
                     /locus_tag="PC472_000245"
     CDS             complement(76162..77958)
                     /locus_tag="PC472_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2725621.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(78172..79101)
                     /locus_tag="PC472_000246"
     CDS             complement(78172..79101)
                     /locus_tag="PC472_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2725622.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(79101..80918)
                     /gene="uvrC"
                     /locus_tag="PC472_000247"
     CDS             complement(79101..80918)
                     /gene="uvrC"
                     /locus_tag="PC472_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2725623.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(81177..84002)
                     /gene="uvrA"
                     /locus_tag="PC472_000248"
     CDS             complement(81177..84002)
                     /gene="uvrA"
                     /locus_tag="PC472_000248"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2725624.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(84021..85991)
                     /gene="uvrB"
                     /locus_tag="PC472_000249"
     CDS             complement(84021..85991)
                     /gene="uvrB"
                     /locus_tag="PC472_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2725625.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(86178..87440)
                     /locus_tag="PC472_000250"
     CDS             complement(86178..87440)
                     /locus_tag="PC472_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2725626.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(87503..88807)
                     /locus_tag="PC472_000251"
     CDS             complement(87503..88807)
                     /locus_tag="PC472_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2725627.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(88838..89698)
                     /locus_tag="PC472_000252"
     CDS             complement(88838..89698)
                     /locus_tag="PC472_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2725628.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(89742..90611)
                     /locus_tag="PC472_000253"
     CDS             complement(89742..90611)
                     /locus_tag="PC472_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2725629.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(90595..91665)
                     /locus_tag="PC472_000254"
     CDS             complement(90595..91665)
                     /locus_tag="PC472_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725630.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        28598 a        17588 c        12888 g        33035 t
ORIGIN      
        1 ctgttgctcc tgttgctcct gttggacctg ttgctccatc tgctcctgtt gcccctgttg
       61 ctcctgttgg acctgttgct ccatctgctc ctgtatttcc tgttgctcct gttgctcctg
      121 ttggacctgc tactccatct gctcctgtat ttcctgttgc tcctgttgga cctgttgctc
      181 catctgctcc tgttggacct gtcgctcctg ttgcccctgt tgctcctgtt attcctactc
      241 ctgttgggcc tgttgctcct gttggacctg ttgctccagg tgttcctgct gctcctgttg
      301 ctcctgttgg gcctactaat ccatttgctc ctgttgcccc tgttgctcct gttggacctg
      361 ttactcctgt tgctccgtct gctcctgttg cccctgttgc tcctgttgct cctgttactc
      421 ctactcctgt tgggcctgtt gctcctgttg gacctgttgc tccaggtgtt cctgttgccc
      481 ctgttggacc tattaatcct gttgcccctg ttatccctat tcctgttgct cctgttggac
      541 ctgttgggcc tgttattcct attcctgttg ctcctgttgc tcctgttggg cctgttactc
      601 catctgctcc tgttgcccct gttgggcctg ttgggcctgt tactcctgtt gctccatctg
      661 cccctgttgc tcctgttgct cctgttggac ctgttattcc tattcctgtt gctcctattg
      721 cccctgttgg acctgttgct ccaggtgttc ctgtcgctcc tgttggacct attaatcctg
      781 ttgctcctgt tactcctact cctgttgctc ctgttgggcc tgttggacct gttactccaa
      841 atcctgttgc ccctgttgct cctgttggac ctgttattcc atctgctcct gttgctcctg
      901 ttggacctgt tggacctgtt actccatctg ctcctgttgc tcctgtcgct cctgttgggc
      961 ctgttggacc tgttactcca aaccctatag ctcctgtcgc tcctgttggg cctgttgccc
     1021 ctgttgctcc tgttggacct cttaatcctg ttgctcctgt attccctgta tttcctgttg
     1081 gaccagtcgg accggttggt cctgttgggc ctgttgcccc tactcctgga cctgttggac
     1141 ccgttggacc tgttgggccg gttctacctc ttggacccat tgggcctgtt ggacctacac
     1201 atgatggtgg acagcaatga tggcaatcac agctattata atcatcacga ccattgttac
     1261 aatcatcata tttatcatag ccattgttgt aatcattatc atcaaatggt ccaaaatatt
     1321 tatttctact cattattaaa agcacctcct gatatatttg agcttttaag ctctatatat
     1381 aatatgtcaa atttagtatg attgtgacct atcaactatt tttaaattta ctaaaataat
     1441 tttcattgtg caaaacagca ctatcgttag gtttgatttc ttttgttttt tcatgataat
     1501 aaatagcttt atctgattct cctaacctgt cataacagac acttaattgt atagatggta
     1561 tatatccata acaatcattt gacttaaagc ctccattagt atcattctta tctcttgtta
     1621 atgctacctt ataccaaaaa atggcttctt tatatttttg tctatcaaac atatgctttc
     1681 ctatgtcaca acaaatttct gctcttggct catcaaattc gaaacttcta aacaatgaat
     1741 ataaggcact tttttcatca ttaattaagt aataacatgt ggctaaatct ctgcaagcac
     1801 tgatacaatc ctcaatccat cctcttgatg agtctaaaaa cttagtaaaa ccttctattg
     1861 cttctttata cctagcatta tagtacaact ctctagaata atagaattgg tgtcttggct
     1921 ccaatgtttt tccttctgaa atcatttttt caaaaattct aagatttctt agagggtcct
     1981 gtctatgaag ctttttatga gatattgcaa tgtcagaata aaatacctct ccaaaaagag
     2041 gtactacttc atgaataggg tcaacccatt tatgattaga acttctttta aacaatcgtt
     2101 ctctataata tgagagagtt gggttaccat tttcatcaaa ggatacattg tattttgcca
     2161 taacaatatc aatggaagta tccaagcttt ctttaatctt taataatttt tctctatcag
     2221 cttctaaaat tatgtcatct gcatcaagcc acatagtata gtctttagaa gctttagaaa
     2281 atgaaaaatt tcttgcagca gagaaatcat ctatccattc gaaatcgtac accatgtctg
     2341 tatattttga gactatttct tttgtggaat ctgtagaacc ggtatctaca attataattt
     2401 cgtctactat atcctttaca cattctaagc atctgcctat aacttcttcc tcattcttaa
     2461 caatcatgca caaacttatt gtaatcataa tgtacaactc ctttgtcttc tatatattgc
     2521 agacataagt aatatatggt attttctctt gttttgttat taaatttata aaaacttttg
     2581 acgacagaaa gttactaaag aaccttatct tttatgtatt ttttatagta gaatttatta
     2641 aacttgaaat ttggtaataa ttgtcgaaat gataaaacca tatttaattt catacaaaaa
     2701 agagtgtccc ataaactaaa aagttcattt tgagacactc ttttttaata ttatatatat
     2761 atttgtaaaa cagacatttt aatatgatac tttgatactt tattcattcc tattcattat
     2821 ataatcaatc agcccgtact ctttagcctc tagtgcagtc ataaaattat ctctatctgt
     2881 atctctccta atatcttcta ttgatttacc tgtattttca gaaagaattt tatctaatcg
     2941 ttcttttatt ttcaatatat tatctacagc tatttttaca tctgttgctt gacctttagc
     3001 tcctcccatt ggctgatgaa tcataatctc tgaattgggt aaagcacatc tttttccatg
     3061 tgttcctcct gcaagtaaaa aagcactcat gcttgcagcc ataccaatac aaatagtcga
     3121 tacatcacat ctgacataat tcatagtatc atatatagca aatcctgatg tagcagagcc
     3181 accagggcta tttatatata taattatatc tgaatctggg tcttctgatt ctaaaaacag
     3241 taattgagct actactaaac ttgcaatagc atcattaatt tcttctccta taaatataat
     3301 tctatctttt aataattttg aatatatatc ataagacctt tccccttgac ctgtttgttc
     3361 aattacatgt ggcactaaac tcatgctact ttcacctcac aattaatatt aaagttaaga
     3421 taagaattga tttcatctct tctttgttta taattcatat ttgcataaga aatacatttt
     3481 tcactttgaa tttgatgatt tagatttaaa acaaatctct gttgaatagg ataaaattca
     3541 tctttatttc tatatacttg tggagattct ttatataaat ttctaaatgc tagtgtaaaa
     3601 gattgatgtg tagaatatcc tactgtaata gctatgtcta taatatattt gtctgtaaat
     3661 ataagttgtc tagctgcttc agtaagtctc ctcctctgta tgtattgatg gatagtacat
     3721 cctacaacat ttgtaaacat tctgtgtaaa tggtattttg aataattagc aatataagct
     3781 attttatcta aatttagatt ttctgtaaga ttttcttcta tataatcaat tatctccata
     3841 ataacatctg tatttgtttt caactctatc ctctcctttt aagtattata acctatgcaa
     3901 aaagtttttt cttaatataa attgctatct taatttagtg tatagcaata aagtatcgat
     3961 aaaaaaatat caggtatttt tatacaaaaa tattaatata tatttctttt aaagtttaaa
     4021 cttaattaca tctattatat gtagattgct acatgtttta tctatgtacc ttgattcata
     4081 tgtattattt tgctgagtat ttttacacct ttttcaattt catcaaaact aagatgagca
     4141 taacctagta taatttcatg gtcatattct aatatattct caattgcata ttgttgtata
     4201 ggataaactt ttatacctgc ttcaaaaatt ttatgaatta aactttccgt aaatttaaca
     4261 ttatagaaat gagcaactat atgaagccct gctgcttcac cttttatttt aaattcattt
     4321 ttaaattccg tgtttaaaac ttttatgagg tacttacgtt ttctattgta caacttcttc
     4381 attctaaata tgtacttctc taaacctcca tttccaataa attcagctaa tacatgttgc
     4441 aatattgttt ctgtatgaac atcagagtac atttttaagg ttttatagct ttctaaaata
     4501 ttgtctggta ataacataaa tccaacacga atagctggag ataatatttt actaaaagaa
     4561 cctatgtaaa ttactttatt tgggctaagt tcataaagag aactaattgg ttgaccctca
     4621 tatctaaatt cactatcata atcatcttca attatatagt aatcattctc tgttgcacat
     4681 ttaataagct ctagtcttct ttgaataggt aaaatccctc ctaatgggta ttgatgagaa
     4741 ggtgtagtat aaacaaaaga aatccccttt attgatttta ttaagttggt attcattccc
     4801 ttatcatcta ctttaattcc aacaactgaa tatccaatag atgaaataac ctttaataag
     4861 cctttatgaa tagggtcttc tacagcaacc tttttattac ttttgtataa aagctgtgaa
     4921 attaaagata aaccttgtgt tgaaccagaa acaatcataa tgtttttggg attacatcta
     4981 attcctctag tacgaaataa atacttagat atttccactc ttaaaaccca tacaccagct
     5041 gtacttccat atctgaaatc cgaatctgat aaatcataca gtatttgttt atatagcttt
     5101 gaaagttctt tgcgtggaaa tagttttaat tctggaactc ctgaacgaaa atctatataa
     5161 tttttttgtt tattagtatg taaatctttt tcatctctaa aagtatcatt taacttaaaa
     5221 tcaactttat gccctgatat tcctttagct acaattgttc catatccatt ccttgtttcc
     5281 aaatatcctt ctgcaattaa ttgattatat gcatctaaaa ctgtattacg agatacagat
     5341 agttcttttg ataaagtcct agatgatggc agtttttgct caaactttaa gcttccatcc
     5401 agaatcatat cttttatctc acaataaatt tgtcttgata atgatatatt actatttcta
     5461 ttaatcgtaa tccacattct aatacctcca aaagtggttc tataaaatta ctataaaaat
     5521 ggaactataa aatccacttt taatttgcta agattatatc ataaaatata aaataaaaag
     5581 gagtctatat tatgttagag gtatcacata aagttaaaga aatttatccc aatattaaat
     5641 ttggagttat ggttataaat attacacatt caaaacctaa taaagaaaat tttttagttc
     5701 ttaaaaactc tacaattaaa aatattatag aacaacaccc agaatacaat cgcaaagaaa
     5761 aaattaaaac agagcctgtt tcaagttata taaagtatta taaaaaattc aagaaaactt
     5821 accctgtatt gcttcaatta gaatctattc tattaaaatc aaaaggtatc ccagatgtag
     5881 gtgtaacaat tgagtcaatg tttttagcag aattgaaaaa tcttcttcta acagcaggtc
     5941 atgaccttga taaaatggaa ttacctttga aaatagaatt agcaaatgga agtgaacatt
     6001 tttatggtat aggtggtaaa gaacatattt taacaaaaga tgatttattt ttatcagata
     6061 atattggtat attatctagt attcttaatg gccctgataa tcgaacagct ataacaaaag
     6121 atactaaaaa tattatgtat tttgtatatg gacctgataa aatatcagaa aagcaaattc
     6181 aagaccattt aaatgacata aaacactata tttcaagtgc ttttccagat ttaaaagtta
     6241 attcaattga tatattttag ttaatatatt aaaatcctca taaatatttt gaataaataa
     6301 attagtgaag tatacattct agttaaacta gatatacttc actaaaatta attatatgta
     6361 gatattattt attttgaatt ttgtttataa taaacacttt attacattta tcctaatgaa
     6421 taacttaatt ttataattaa agagtttcat aagaaacaat ttcacttaaa ggaacacgag
     6481 ttttttcatg tctctctggg ctctctggta tcttagattc atatcccatc aataatatat
     6541 ttattggttc tagattatct ggcaaactaa actcttctct aattatatct ggattaaaat
     6601 agcacaccca aacacttgcc aatccaagtt cagttgcttg taacatcata tggtctgtaa
     6661 ctatacttgt gtcaatatct gtaagttgtt tcccatcaaa aggtcttgtc caaactttgt
     6721 ctttatctcc acatacaaga attgctaatg gcgcatcata tatattggca gccttagaaa
     6781 gtttatttat accttctttt tcctgaataa caatcaatct ttgaggttga cgattaccac
     6841 cagttggagc tattcttgca acatctaaaa ccttctcaag tttttctttt tcaacctttc
     6901 tatcttgata attacggcaa gaatatcttt tttttgcaag ttcaacgaaa ttcatttatt
     6961 tacctcctat tgttttatta ggctaagttg atgatttatt taacctatgc tataatttta
     7021 tcactatggt actatattac aataatgcac ttttttgtaa tatagtacct aaaaagatac
     7081 tttaaaataa ataaaggagc ccaaaaatga aaaaatgttt agataattac agttgcccaa
     7141 tagaagcaac acttgcattg attggtggta aatataagac acttatactt tggcatctta
     7201 aagacactat attgaggttc aatgaattaa aaaaattaat tcctaaagca acgcctaaga
     7261 tgttaactca acaattacgt gaattagaat cagatggttt aattataaga gtggtttatc
     7321 ctgttgttcc tccaaaagta gaatactcgc tatctgattt tggaaagagt attattccaa
     7381 ttttagattc catgtgtgac tggggttctg attacttaga aaatctataa accattacag
     7441 tttaaatccc attgctaatt taacttcatc catttgttct ttagatgtca acatttctaa
     7501 aagcttaaaa gctacatgtg gagctgtttc tggacaatat gaggttataa tatttttatc
     7561 tacaacaatt ggttcattaa ctacatttac atcaaactca cttaactgtc tttgtctttt
     7621 accatttttc aaatgatatg tagtagcttt acgatttttt aaaacacaac tttttccaac
     7681 tggcaatgct gccacacaaa tagaagcgat tattttttct tttgaattaa actcccttat
     7741 tagatttaaa aaagatgaat catatgcttc atcataaaat ccaaattctt caaatcctcc
     7801 aggaatagct agagcatcat aatcatcaac acatacttct tctatcgtct tatcaactaa
     7861 tacttgtata ttaaatgtac tcataacttg ttttttaaat ccacaagtca ctacatcaat
     7921 atcatgccca taatcgttac gagcccatcc cataacatct acaaatacac taaattccat
     7981 agtttcaaaa ccttttgcta aaaaaactaa aactttcata ttctattttc ctcctaaaat
     8041 ataattaaca ctttactagt aaattattta aaaaatatct tttattgtat taaattcttc
     8101 tttaattgta gataaaacaa tggttgtatt agctttttga atccccccaa tttccttcaa
     8161 agtcactgat ataaactctt caagactact tgtatcttct gctaatactt ttattaagta
     8221 atcatactct cctgaaatat gatgacattc taatacagta tcatattcca atatagtttt
     8281 cttaaattct ttcatacttt tagataaatc agtattgata aatataaaag ccaaaagctt
     8341 atattttgtt ttttcccgat taatttgaat agtgtacttt tgaattaaat ttgactgctc
     8401 aattttcctt atacgctctg agactgctgg tactgataaa ttaatttgtc tgcttatttc
     8461 agaggttgaa gccctactat tcttttttag agaattaatt atttttaaat ctatttcatc
     8521 cagagctact cacctcctta attatttaat acaattttag ctaataattt acattaagta
     8581 aataaattta atcatttcaa taaaatttaa tacaaatata ttatttttcc ttaaaatttt
     8641 agttttcttt aatcaaaaca catataatac ttaaaattat ttaagttgaa ttaatgtttt
     8701 tattttgtat aatatattag aaccttaata tatcattgaa aattcaacaa tactagacat
     8761 atacttctac atgttgtaat atttatgaaa atagtatccc tatatcaaaa ttataaaact
     8821 cataatctta attttcttat agactataaa attttattga tagcgagtct tcaaataact
     8881 ttgtaatgat agatttacta aattaatata ataatagtaa atataacttt cttattgact
     8941 ataactaagt attatgataa cataacttaa gctataatta ataaaacata ttttaaataa
     9001 ataagataat ccacaaagta caaaagtaca atttaaatat agataaacta gaattaatac
     9061 taagattagg atgtgaataa ttatgattta tatagaatta gttaaacttg ttagaaaact
     9121 taactctaca caaatgtata cgatgaagcg ttgtgtagag gttaatctga aagcttaaag
     9181 ctttctatct tcatcggcaa atataggttt agtatagttt tttggctatg cttatttaaa
     9241 attacctatt tgccgataga taatatagat tttctggtca tagacaacaa tgcttgtcta
     9301 tgaccttttg tttttccaaa agcagaggtc ggcattttac aatgccattc ctctgctttt
     9361 tttatttcca taaatttttt aataaaagaa aaggagtttg aattatgagt ttattaaaag
     9421 taactaattt atcacaatgt tttatggata aatcactgta tgagaaagct aactttgatt
     9481 tattcaaagg tgaacatata ggtgtagttg gccaaaatgg tacaggtaaa agtacattaa
     9541 ttaaaatttt attgggagaa gttgttccag attcaggaga aatcaagtgg caacctaata
     9601 tcaatatagg ccatttagac caatatgcag aaatcaatag agatacaaca atatcactct
     9661 atttacacac tgcatttgaa gaactttata aaatagaaaa ggaaatgaat ttactctatc
     9721 aaaaaagtgc tatatctgaa aatgagcaat atctaatcaa agcatcagat tatcaggaac
     9781 aactaatagc aagtaatttc tattctatag ataatgaaat aaataaaata gcaaatggtc
     9841 ttggattaga ttcaatagga atgaatcgtg ttgttggaga gcttagtggg ggtcaaaggg
     9901 caaaggtaat tttagcaaaa ttactacttt ctaaccatga tgttctatta ttagatgaac
     9961 caacaaactt tttagataaa gaacatgttg aatggctctc aaattattta aatacattta
    10021 atggggcatt tatcgtagtt tcccacgatt ttgacttttt agaaaaaatt tctacaggta
    10081 ttcttgatat agaatttggc atgattaaga aatatcatgg aaaatattca gagttcctta
    10141 aacaaaaatc acatttaaga gaagattata ttagaaggta tcaagcacaa caaaagaaaa
    10201 tagaaaaaga agaaacattt atacgtaaaa ataaagctgg agtaaattca aaaattgcaa
    10261 gaggaagaca aaaacaattg gataaaatag aaagaatagc tcctcctagt tttacaggaa
    10321 aaccaaatat tcaattttca gaaattgaaa tatcggctca aaatgctctt actataacaa
    10381 atcttgaagt tggatactac tattctttac ttccaaaatt aaatttttct gttgatggag
    10441 gtcaaaaaat agttataact ggttttaatg gaatcggtaa atctaccttg ttaaagacat
    10501 tagtaaaaga tattcctcgt atatctggag atttccagtt ttcagaacaa gttaaaattg
    10561 gttattatga gcaagattta aaatgggaaa atcctgataa aaccccactt caaatagtag
    10621 cagataaata tccaaaatta aatacaaaag agattagacg tcatctagcc agatgtggtg
    10681 tgaaggaaga gcatgtttca aggagtgtat ctactttaag tggtggtgaa caatctaagg
    10741 ttaaattatg ttgtatgatg ttatctcctt gtaattttct tattctggat gaaccaacca
    10801 atcactttga tgcagagaca aaggatgctt tacaaaatgc tttgaaacag tttagaggaa
    10861 gtataatcct agtttcccat gaagaaaaat tttataaagg ttggattgat aaagtattta
    10921 atatagaaaa acaattagta taagtttata cacaatatac aatattattt aattggtatt
    10981 tcaatgcaaa ttttaatgtt ctagttaatg tttcactgca aataaactaa aaaaatttta
    11041 ttagaacata aagtacatta ccaggatata aacttaatga ggctatctca aaatagagat
    11101 agacctcttc ttcattgttg agataacgaa aatacatttg atttcaccca taaaagaatg
    11161 cctcattaac taaaaattca acttgagata tcttcattaa tattaaaatc tattctaatc
    11221 gattctattc acaagtttat atcctagtcc tttaactgtt ataagtattt taggatttga
    11281 ggggttatct tcaatttttt ctcttatatg tcgtatatgc accataattg tattgtcaca
    11341 aatactacta tatactcccc atacttgttc atagagtctt tcttttccta taattttatt
    11401 tacattttga gccatgtagc ttaaaagatg atactccctt ccagttaact caatctcatt
    11461 tctatcttta tatacacggt ttccttctat atcaatagta atatctccta ttttaataac
    11521 ttcactatct gatggtacaa tgctttgata ctgttgtcta cgtaattgcg ctttaactcg
    11581 aaaagcaatt tctcttggac tgaatggctt tgttatatag tcatctcccc cactactaag
    11641 acctagtatt ttgtcaatat catcattttt tgatgataaa aaaagaatag aacagtatga
    11701 aaactctcga atttttttac acacttctat accatctata tctggtaaca ttatatcaag
    11761 aatgacaaca tctggacatg ctatcttaca cagcgaaata gcatctagac cattataagc
    11821 tttaataatg tttttaaaac catcatttat gagtacttct tcaattaaat ctactatatc
    11881 cttttcatca tcaacaagta atattttttt attaaaacat gtatccatat tgaaatctct
    11941 ccataaaaat ttattatatt ctaatcttgc agtatcacta tcttgttttt tatattcact
    12001 actcttgctt ttacattact aatagtaaca tctctaatag acaaattcaa tcactatgaa
    12061 aattaagata agtttcttta aaataaagtg tatgatacat taaaacctat agaaaattaa
    12121 tttcatccaa agtttataat attagactaa tacacttaca gatacagatt aatacaatag
    12181 taaaataaaa ggagttgtct taaaattgac tttttctaat caaactttaa gttcaactcc
    12241 cttttaaact tttatatgtt atttttatta attctttata tcaattataa ctttaatttt
    12301 caatagtata tatctcccac taattgaatt acttattaaa acataaaata tctacctaaa
    12361 ctatttattt aatatctaca tatatttata tctattctta tacttacata tacaaaaaat
    12421 ggttaaaaat aaagttaaaa tctcagcact tggtactgct aaccatatcc catttatatt
    12481 taatatattt ggcaatatca ataaacctat agttataaat ccaaatgtac gtagaaaaga
    12541 taatattgca gacacctttc catttgataa tgctgtaaac atagacgaag taaaaatatt
    12601 atacccacaa aaaataaaac taaaagaaaa tatataaaat ccagaccttg caatttcata
    12661 aactcggctt cctctttgag aaaaaatctc tacaatgatt ggtgaacatg atatagaaaa
    12721 gataaatacc aataaagatg ctactgaaat aaataatata caaatcttaa aaacatgatt
    12781 taactgcgta ttattttcac ttccatagtt ataacttatt attggtgcaa cacccattga
    12841 gaaacctata taaagcgaag ttaaaagaaa ttgagaataa ataattattg taatagctgc
    12901 aaccccatct tcaccaatta atttcatcat catagcatta aataaaaaag tagtaactgc
    12961 tgttgatagt tgactgacca tttcagatga accattaaaa caagcttcaa ataatattct
    13021 tttatctagt ctgggtatac aaaacttaag taaccctttg ttttttgaaa agaaaaccat
    13081 acctgaaacg gtaggaatta ggtatcctac tccagttgca agtgctgctc cactaatacc
    13141 catttgtaac agaacaatga acacatagtc aagaattata ttagcacacc cagcacctaa
    13201 cactagccat aatccaaatt gaggctttcc agctgttaca aataaacatt gaaataatac
    13261 ttgtagtata tttgcagaag caaaaaccaa gataataaaa agatatgaac gacaatattt
    13321 aattaaaact tcacttgctc ctaaccatat aatgatataa tccataaata caatacctat
    13381 accagtaatt attacaccaa ttacaactcc tgataatacg attaaagaaa aagctttatt
    13441 tgctccctca atatcaccat ttcccatctt acgtgctaca atagcacttc ctccagtggc
    13501 aagcatagtt ccaattccaa ctataagatt aataattgga catacaatat taattgctga
    13561 tagagcattt gtattaataa atcttgatac aaatatagta tctacgattg tatacaaccc
    13621 cataaaaatc atcataaaaa tagtaggaaa tgcaaacttc aatagggtaa ataaattaaa
    13681 tctttgtgcc agtaaattat cttgctcata aagattatct tgttcatgat tttctgaata
    13741 atatacatca ctcattttgt ttatttttct gtacattaat tatttttaaa atatattcca
    13801 aacaattaga catttacaga cctcctttct gcataagaat tatatctaat tatttggata
    13861 tagttttaat taatcttgct tttttacaca attatatttc tactaaagaa acttttaaat
    13921 ccttacttta ataattttat agctttcagt gcctttagcg caaatacttg ttcacatagt
    13981 ttagtgtcat atgaaacata tttatatata gttatacatt gtctattttt actaatttat
    14041 taactacttg cacttttata atgtctcata cctaacttcc aaaatctcca cataaacaat
    14101 gtaaacaaaa atacaattaa aattgagtat attaatccat taattgttat tgtcccagcc
    14161 aaaatttgag tcggaattgt cgacataatt ccatatggta agattaaata gaataatact
    14221 ttaaaaacac cttcatatat tacaccagga attttcatat tcattggcaa taaattatct
    14281 tctaatctca ttatcgcaga agatgatata acaaaaaatg aaactgttct aagtatgata
    14341 catacatcat aaaaaagtaa tgtcatcaat aacaccatga aaatatatat tattatcata
    14401 gctaaagtta tctctgagtt taactcagat attccatata aaacaataag tatacttgct
    14461 agaattaatg gtgatgaacc aatattaata ttttcaaatg ttaacctaaa tagtggatct
    14521 aatggtttta taatatagta gtctaattct ccttcctgga tttttcgtgg aatatcataa
    14581 ataccaaaaa agaaaatcgt catatttatc gcatttatta atgagaaagt tcctataaat
    14641 attaacatct ctccttgttt ccatccccct attgagtcta catgagaata tatactatta
    14701 aacattagaa gttgtaatat aaataatgaa ctatctacaa aaaatgctcc aaaaaaatca
    14761 aatctaaata ccatcatctt tgataatttc atttttaata acataaatat tattttcaaa
    14821 ttttttttca tcatataccc accccatcaa ataatgttct cattctttta tacataactt
    14881 tatttactac agaaaaaaat aaaacccata atgctagaat aattaatcct tgtattgcct
    14941 cattgctgtt tctacctatt agtaacatag atggcaaata tgttgtataa taaaatggaa
    15001 ataccttcat aactgttact atagtttctg gtaagagtac aagtggtatc attgttcctg
    15061 taataaattc aacaatattt tcaattatca ttctaaaaaa actaatctca agaaatttaa
    15121 atgacaaaat cccaataaag aagtggagtt tcatcataaa aataaaccct agaaaaatca
    15181 tcaaaattga aattaatatt tgaactggat ttgatgtaaa ggcaaaatct attttaaata
    15241 taaaaatcca tacaaatgca gaaatcatat taaatacaaa ataaaaacat gtcattccaa
    15301 aatttttagc aataaaataa ttttgaacat taattggaat aatcatgtac tttgaaaagg
    15361 tgcccaattt tacacgatta catatctctt cactggtact ctttgacata tcaatacctg
    15421 ctaaaaatga gcttattata tagtaagata acatagagtt aaatgtaaaa ccagagacta
    15481 ttttattttc accaaaaatt gctttccata aaatataagc aaataaaatt tttacaatcg
    15541 taaacaccat attaaccacc acatcaaatc tccagataag ttgcacttta aaattaattt
    15601 tggctacttc ataatacctt ctaatactct gtttaaattt ataaaacatt gaataatttg
    15661 aacttgcttc attatagttg ctcataatac tcatcacttc ctttcttata tattctctct
    15721 acaatactac ctatatcttc ttcttcaatc attacatctt taacatcata tttatcaaaa
    15781 atccatttaa tcgtttcatt tcctctttct tttggcacaa ttactgatgt cttatgattt
    15841 gtttgttcaa caactttaca atcttttgga aatacaatgt tagtttcatt ggaaaaacta
    15901 actgttattt ttttatattt ttgaaaatga ttaaataatt tctctgtaga accatcatat
    15961 attttacgac tattttctac aacaatactt ctactacaga gactcttaat atcttccatg
    16021 taatgagaag taagtataat agtagttcct tttgtttcat ttacttcttt taggaaatac
    16081 ctaatttgtt tttgggctat agcatctaag cctatagttg gttcatccaa aaataatatt
    16141 tttggattat gtagaagggc tactattagt tccattttca ttctttctcc taaagaaagc
    16201 gtcctaactt gaacatttaa atagtcagat acctcaaata attcgataaa gtaatccaag
    16261 ttttttttat attcttcatt agatatatca tataattctt taaataaaag aaacgtatct
    16321 gctggtgtca attcaaagaa tagttgactt ttttggccca taacaacagc atattgtttt
    16381 ttaaaattat tttctaattt gttagggtaa taacccatta cagatatttc tccagaagac
    16441 ggtgcaataa taccagttaa catcttaata agagtagttt tgccagctcc attttgacct
    16501 attaatccaa caaattctcc ttcttctatc tttaaatcaa atgacgatat agctgttttt
    16561 tctgtatatt tcctattaaa taaagacttt acactcccca taaatcctgc ctttttctca
    16621 aatcttctat aagatttagt cagttcatta agttcaatta tactcatttt aactacctcc
    16681 atatttcata atttatataa aaaaaagtcc acaggtatat atctgccttt ttctgtgctt
    16741 atatttaaca gcacaaaaac agaaaactcc tgtggactag aaaaattaat gctctctatt
    16801 tcaaaataat tatcaaaata atactataca aaattaataa aagtgtagca gattaaagcc
    16861 tactacacca taatttaaca aagtaatata tattttaaat actatttgtt gtcatatgtc
    16921 agttatctat aaaggcagaa taagacacaa aaattaaatg tattaaaaat acacttgaaa
    16981 tttctgatta tacagaatat atgtgtctcc aactatttaa ttgttggtta ccattatata
    17041 actccgacat caaggcactc ctctcataat attattattg atatcatatc ataaaggtta
    17101 tttatgttca agcatggatt tatatactaa tacatttagc ttatttcttc aaggttgaat
    17161 ttaaatttca tttataatat tcgtttataa attaaaaata taatacgtat attaaaactg
    17221 gtcattttac tgtaatattt ttattgtaaa agatatactt ttacctatac aggatgtaat
    17281 ttctttagtt ctatattata ataatattgt aataagagtt caacttataa tacattaaaa
    17341 tataaactgt aaatcttaag ggggtaatct atggaaaata atacaataca aaaacaatta
    17401 agagatttat caaaaaaaat ttggcatatt tatattttaa atccagaaga agaaaacctt
    17461 aaatttatta tttccttatt agatgacaac cttgcattaa ttggaacagg aaaacatgaa
    17521 ttttacgaaa cattagaaga atttattata ggtcttgaaa aagaccaaac agaatcagag
    17581 caaatagact ttgagattat agatgagtgg tattcatgta ccaaagtcac agacgaagct
    17641 tttatagtat atggtactct ttgggtaata gaaaaagatt acctagaaaa agatatctat
    17701 gtagaaatgg atactcgatt tactatggta tacagagtta cagatgatat gattaaactt
    17761 atccatgttc accattctat tccaaacttt gaccaaatga aagatgagta ttaccctaaa
    17821 acaataactg aaaaagcaca agaagcacta gcaatggcaa atgaatttaa actaaaatca
    17881 gaaaaagacc ttatgactgg actatacaat agacattttt ttgaagtaca cataaaagaa
    17941 atgcttaaaa ctgtttcaca tggatacttt tacatctttg atttagataa ttttaaacag
    18001 gtaaatgata tctttggtca tgttcgagga gatgaaatac taatacaatt ttcaaatatt
    18061 ttgactaaag tattcgtaaa tgatgctatt attggaagac ttggtggaga tgaatttgct
    18121 gtttttgaat atagtaatca atcaaaagag aatgttgaaa ataaagcaaa gaaagtttta
    18181 gaattatgcc tagagagtaa ttcttgttct ataggtatat ctaaattaga aaataactct
    18241 gatactttcc agtctttata caaacaatcg gattatgctt tgtattattc aaaacgaaat
    18301 aataaaggaa actttagttg gttttagtgt aaaagatatt aaaataggca aatttattag
    18361 agtatcttgt cctatatact aataggtaaa gtaaacaaca agctatcttt gcatatattt
    18421 atataaacca actattttta ataaaactac aatagtcaac atgaccacta ttatagaata
    18481 taataaacta aaaacattat taacatgatt aatattacat tttaataatt ttatttttaa
    18541 aagtaaatcc aaaatgttat attttttgat taaaactgat gttgatataa aaaattttac
    18601 ttaaaaaaac atttagaaag gctttataaa gagccttttt tgcttttatt gattaaattt
    18661 tcatataaaa ttccttatta aattttaaat caataaaaaa ggctccataa gaagccttcc
    18721 ctatttttat tgattaaatc ctaatataga caatgcctta gcatcatagt aagtagtcat
    18781 ctctcttgtt gcatccttta atttacggta atcaaagtct aatgatccac caaaagctga
    18841 tatttcagac ccatcaaacc tataccattt cccatcaatt tgtatatagt tccaagcatg
    18901 atttaatgat ggatttaata tagatagtga atccattccc attaaccttg ttaataaatg
    18961 aaagctactt gcaaaacttc cacatacacc acgtttatca accagattac catagactat
    19021 gttacaatat ttagagtctg catattttgc ttcggttacc aattttgcag ctctattagc
    19081 tctttccatc tcagatgcat ttctccagtc aaaactattt aaaaaattcc tgattactgt
    19141 aagtgtatct tgagcttgtt tgtaagaaat ccaatttcca tcagctatta cttctatagg
    19201 gtcaagtttt ccaaagccta ttctctgata tgagacacct gacaatgctg ccgcataaaa
    19261 tcctagccct tccataccct tatttatata ataatattta ccatctgctc gaagctcaac
    19321 tccattggga tttcctccat agagtaaatc ccatccctct ttacctgttg tattaagacc
    19381 aagttgtgtt aacattcctt taagaggata tttagggtca taattagcac ctttacttat
    19441 tgcatttgtt tgcactctgc catccattgt gcctatatta gtctgattac gttcatttgc
    19501 actctgtttt tccaagctag atgtttcatc ttcttgatat ggctctgcca ttactgtcat
    19561 actcatcatc aatataataa tcatggtaat catagtagtt aatatattct tcttcatact
    19621 actccccctt atttaattct ttttaaaaca taaaattatt aactcataaa tatatggtta
    19681 ttagtaaatt gaaattaagt ctgattcata aattattggt ttaatagaag tgtattgaaa
    19741 ttgtaccttt atattttcat tatataataa acttattttt ttaaaacata ttgtttaaat
    19801 cagtacattt atatgttttt tttgttcatc tgttttatca aatatattag tctttgaatt
    19861 ttgtttgtat tcttcagcat ctttatctaa cttttctatt ctcgactcaa gtttcattat
    19921 ttcttcttgt gttaaattta taaaattttt aagcatattt tcaagctctt cacgagtttt
    19981 acctactaaa gataagtcct cagatacttt ctctttttta ttagaatcct ctttataatc
    20041 ctttttttgt tctaactctt ctaatttttc atcatttaat atttcttctt caattttttc
    20101 attagactct ttagtgatta aagattcttt ttcatcattt gtatttagat aagttgaatt
    20161 ttcatctaac attgtacttt tatttttatc ttctataatg ctatttttaa tacctatttc
    20221 tatattagaa aagttattag ttgtttctct tatttctgat ttacataagt tcttaattgc
    20281 gtcatttatt tttttagtat aaacctcttg tctatatttt aaatctctaa ttctatcatt
    20341 aagttcttgc ttttgtttca taatattaga taaataagtg tcttttccta cagtttcatt
    20401 tttcttaacc atactttcat ttattcttgt attatttata ttactttcat atctaaatga
    20461 atattgactt ctatattgac tagatatttt catttatttc acctctctaa aataaattta
    20521 tgtttataaa aaccaactta gcttaattat attttatcat caaaagtata catttacaaa
    20581 tattagttaa tagttttata ggttcgagca ttattttcaa caaaaaattt ttattataaa
    20641 ttaaaacaat aatttttata aatatattat ttgtatttta taattcatat atttttactt
    20701 tataaattag caatattgca gttttgctct taagaaacat ctctatcata cataagcata
    20761 tactctatta tgtattagta taaatatgcc ttttcttatc ttacataaga cctctttata
    20821 aaggagacta tcgtaacaaa tatgtataca ttatcacatg tatatatcat catataaaaa
    20881 atcaaacttt attaaaaaaa ataataaatt gttttaaata ataccacaaa tcaaatttgc
    20941 catcaaaatc acattaaagt aataaattaa tggcaaattt aatatattaa tagtatatag
    21001 actcatataa tgaacaaaaa aactacactt atcttctaat cttcttattt atttttgaga
    21061 tatttttaaa tttcttttct ttttttgcta agtattcgct tttattgtct gcaaatactg
    21121 cttcagtttt aagcctacga taagactccc atcttttagg cgaaagttct tgatgagcaa
    21181 tagctttcca gatagcacag cctggttctg aggtgtgagt acaattacga aaatgacatt
    21241 ttccgaaata agcttctata tctgcaaaac ttttatctaa accttccgaa acatcccaca
    21301 ttcccaactc tctcatacct ggtgtgtcaa taacaatacc accacctgaa attaaaaata
    21361 gctctcgtct agtcgtggca tgtcttcctt tatcatcatc tatgcgaatt tcctttgtat
    21421 ttaaaaattc tccacccaac aaacaattaa ttaatgaaga ttttccaact cctgaagaac
    21481 caagtaaggc aacagtacag cctggtttaa tatacttaga aatctgttta taaccatctg
    21541 gctctataga tgaggtaaca actacatcta cacccaaggc tatggaatta actacttgta
    21601 atttacgata cacattgtca cataagtctg atttagtaag tactactact gggatagctc
    21661 cactttccca tgttaaagac agatacctct ccaatctatg aatgttaaaa tccttattca
    21721 gtgacatgca caaaaaaaca atgtcaatat tagctgcaac cacctgttcc tcatttacct
    21781 ttccagcagc cttacgcaca aacaagcttt ttctatgaag cacatgatga ataatagcat
    21841 taccattttt attattgcaa cgatccaaca taacgaaatc accaactgta ggaaaatcag
    21901 attgtatagt agcctcaaaa cgcagctttc ctgaaacctc tgcaaacagt acaccattta
    21961 cacatacagc ttgataaaag ttcttttcct gcgagataat tcgagcaagg tataaatcat
    22021 gatattccat actttgagta ataaaattcc tatttagacc atagtcttgt aaattgattt
    22081 gtttcatacc attcattctg tttcctcctt ttccaacttt tcaatgattc tttctagtgg
    22141 gaaagggggc tgtgccagtg gttttaatgc aattgacatt aatttcactg aattatcatc
    22201 agctgcaata cgattagaac taagatgtcc acagcgtgta caacgatgaa taattgccca
    22261 ctcaccattt tttctcaccc acactccaat aggttccata ttagcatcac aatctgcagc
    22321 acggtcacca ggaatgatat ctacatgaag actatgtaaa caatgtggac aatgatttct
    22381 atgttgtgta ccagctcctt caggaactac aagacaacca caatttttgc aaataaagtt
    22441 atccatacag gaattttcct gataataagc tcgttttcta tttttataat tcatttttat
    22501 ttcctccgta tcgtgtattt tctacaaaaa aagtccacag gtatttatat ctgccttttt
    22561 atgtggtata catatttacc tacacaaaaa cagaaaaccc ctgtggacta gaaaatttag
    22621 tatcttctat ttcaacataa ttatattcaa aataatactg tgtaaaatta ataaaagtgt
    22681 agcagattaa agcccactac accacaattt aacaaagtaa tatgtaatat tattatttgt
    22741 tgtctaatgt tagttatcta taaaggcaga gtaagacaca acaatcaaat gtatcaaaaa
    22801 tacacttaaa aattctgatt aaacagaacc catatgcgtc tccaactatt tatttgttgg
    22861 ttaccattat atataactct gacatcaggc aagacactcc ttcactaata attattattg
    22921 aaattttatc ataaaatata agtatgttca agtataattt aatatattca tatatttatg
    22981 tttgtctcat tttcttattc tataaaatct agtggtattt taaaatttaa tgttcaccta
    23041 gtattaataa taaaaattaa ctgctattat ttgttttctc attttatgcc tattgctcaa
    23101 tttatctcac ttaaattaat aacaactgtc ttttcataca aaacctacaa tcaagttttt
    23161 ccatatattc tacatgcata gtctattttt ttgatataaa ataacgttca tatttgtaaa
    23221 tttatttaat tttcaaataa gtatattgaa taataagcaa ttctattgta ttattgtaat
    23281 tgtagaataa aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac
    23341 taacgcttat aaaataaact atgagttatg tcagccagtt gccaaaaaga gatattcttt
    23401 ttgtttttat gaaagttttt tagggggata ataatatata tgtttagaga taaaatggat
    23461 agatgtacac atatgttgac tgcttatatt ggtagttcat atgattattg tgattttata
    23521 gatacacaac tagatgattt tatactagag tacggagaaa atgtggtaga atcttgtttg
    23581 catcaagtga tggtattagt aagtaagtat aattaaatag atttggtttt ctagagaacc
    23641 ctttttagtt ttatctactt taaatagcct tatgttagaa agctaattaa gatagatttc
    23701 tagtattcat aattttattt tttatttaaa atacatataa taaagatgta aatttgtatc
    23761 aaataataaa tatgagaact taaattctct tatctatgtg aaaataaaca tctcttaaaa
    23821 tgaaattctg caaacataaa tgatttttta gtttgaagga ttagattaac aaaattataa
    23881 atttgcaaat aagctatatt aaaaaatata ataatttttt atataactta ttcttttata
    23941 acttttaatt ttaaaagagg agaatgtata taagagtatt caatgtacta gaaaaaatca
    24001 aaagaaatat tagaagaaat ggatattgag taatcaaaga aaagattttg atattacaaa
    24061 tgatatgttt caagaaagca ttcctatcac aacaaaaatt attcttgaag acatgccttc
    24121 aaatgatgaa ttaaatcatg ttttttcaaa aggttgtgaa cgaaaaatga aaaagagaaa
    24181 aattgtatta atcacactac tattaatagg tgttttgtta ttaggaagta tcctatataa
    24241 tcttttttta ggaaaaacag ctaatatttc aatgctaaag gaatcttgga attttgatat
    24301 accaatacct aacaaagaaa ttgaagtatt tgatactcaa gatagtatta atggagatgg
    24361 acaaaattat tttattcaag gattttctga aaaaaatttc aaaaaagttt ttaatttaaa
    24421 aggtggtata gttgtaagca aagataatat taatgaaata gaaaaatata ttgataaatt
    24481 taaaagagat tctgttaaca ttaataaaag taataaaaat aaaattgaag aagattttaa
    24541 aaaatataag ttagaagtta aaaaagatga taagtatata tataagagaa attatgagaa
    24601 ctacgtagtt ttaattataa aaaaagatga acaaaaatta tacagtttaa tatggaatca
    24661 gtaggtacat atgtattatt aagaattata aataataaaa ataaatttta gtcaatatac
    24721 aaaaaccttt taaattgtct ggaaatcacc tcttcatttg ttataaaaac atacaacaaa
    24781 tgaagaggtg attttatggt tttaaaaaaa ataattataa gtggatatgt gctttcacat
    24841 gtttatcaac atttgtaatg cctatatttg ctaatgaatt ataatataaa aatattaaat
    24901 aatattcaac tatagattat gtaaattatt ctatgaaaaa aatgagttgt ataaaatctg
    24961 gctactgtag attgacgatt tcttttaaaa ttacaattat aataagtttt atagaattaa
    25021 aaataaaact ttagagattt ttgataacat tgtagactaa atttgccacc gaaatcgctt
    25081 tgcagtggca aatcggtggc aaatacaata tattattagt atataatcta tataaacatt
    25141 actattgctt gattatctta gctacagttt ccacatgtgt cgttactact atcggaacac
    25201 attttatcaa cctcgttgct gtcatgtgaa cacaattcat tagtcctatg ccgtcctctt
    25261 gcatttttcc tttgtggttt aaatttagca ccatctacgc tattcttcaa gcctgtttta
    25321 taaacaaagg tcaactgtgc agggtcaaca tttccgtttt catcaagtcc acctacaatt
    25381 actttttcaa caatgctttc aaacacatag cggtcaaatt tatcaagaac ctcattctgt
    25441 tccagagttt tcttaaactc tttcaaacgt ttttttatgt ctttctcatt atcagaagtt
    25501 tcttgtaact tttgtctttc ttcaacaagc tgttcttgtt tacttactaa atcagcatat
    25561 ttactttcgt aggtttcctt atcaataatt tcttccagac gcatatccac aagtttactt
    25621 tttttcttct ctaatgcgtc tatctccttt tccgcttttg caagttgttt tgacaccaca
    25681 ctactactaa gagtatcgtc cattctctgc aaaaattctt ccagaacatc tttattatca
    25741 tcacataata aacgataact ttctacaaat gcttcttcaa tagctgtttc tggaatacct
    25801 ttgctatgtt tacaaaattt ctttcctttc ttcgtagccg ttacacattg ccatataacc
    25861 ttactgtatt gtgagccact gtgccaatta cgtcttgtta aagtaccacc acaaaagcca
    25921 cattctagca tacagctaaa tgcgtatttc ctgctatact tctcacgctt accgctgcct
    25981 accgttgttc ggttcttatt tctccgatta agaatttcct gtgctttttc aaaaacttct
    26041 tcactaataa tcggctcatg gtgatctttc atataaaatt tatcttcttc acccatgttt
    26101 tcaaggcgac gtttggaaat cggatcaaca gtaaaagttt tgcctaagag tatatcaccc
    26161 ttgtatttct cattcttaat aattccaata accgtagatg gtgcccatgt ggaactccca
    26221 tacttcgttt tataacctag actttcaagc tcctgtccaa taacagaacc accagcacca
    26281 tcaacataac ggttgaaaat atatctgaca atctctgctt cttcttgatt tacagttatt
    26341 gtcttatcct ctgggtgata atcatatcca atacaacctt gaaaacctac cagctcgccg
    26401 cgcttcattt tcattttcaa cccttttttt acattggctg aaatgttctc gacttcctgc
    26461 tgtgcaacag aactaagaac aacaagcaat aactctccat ccattgtaag tgtattgatt
    26521 ttttcatctt caaaataaac ggcgatattt ctctctttta gcatacgaac atatttcaat
    26581 gtgtctaaag tatttctggc aaatcttgat atggattttg taaataccat gtcaatttca
    26641 ccattcatac agtcatttat catacgctga aaatcttctc gttttgttac ttgtgtcccc
    26701 gtaattgctt catcagcata aatgtcagct aacacccatt ccttgttttt cttaatcatg
    26761 tcagtataat actgaacttg tgatttataa ctgtttaact gatcttcgct atctgtactt
    26821 acacggcaat atgcagctac acgcaaacgc tcaatggctt ttccacgggc ttgtcttgac
    26881 aaaacatttt ttgcttttat tacttcaact tcctgcatag tacacctcct tattttgtgt
    26941 tcctttcata ttgttattgt actactttaa aaaacacaat tcaagcagtt atgtcagaaa
    27001 gcaccttata gtctttcatc agcctttgtt ttacaagagt gtattctttt tcagaaatca
    27061 gtttacgggc aagcagttgt tttaacatag caatttgcat actataccta attatattat
    27121 tcattaaaac cctccttgct tttttattta tggaagtaat cagacggctt cggcagctcc
    27181 actggacttt caccattccc attctgatgg gtgaaccgtg tcatacgggt gtatcattat
    27241 tcagatatac gagtcatggc agcaaccttt ccaaagatcg cttgcggttc actggcataa
    27301 tcgctcgctg ctttccccct ttcagtggtc gtggcgttcc agtccgctgg ctcgtcgtat
    27361 atcaaacgta tctgattact gtattcactt gtcaaagaac ggaaaagagt taaaatgagg
    27421 tggtagatac tctctgttat atttacttca ctttgacttc aaaccctaag attactttaa
    27481 tcaacgctgc ccgtatcctg cctttcagtt ccatatcaag cacgatatac atattcccgt
    27541 gttcgtcata gaacgggcgt aggctggctt ttgatatgta agcgtcataa tggttcaaaa
    27601 tcttttcaat tgccacctcg ttaccgtcag ctgcagagct gatggtcgag aatgatggac
    27661 acttttttgt agacttcatc atgtttttat tcctcctcgt acatatcctt aataagcttt
    27721 aatgtgtgca gcctgtttct acatacggaa aatcgttcca tttccataac ctctgcaatt
    27781 tcagcgtcgg tcatttcaag aaagtaggac ataagcagaa tgttacgtcg gctttctgtt
    27841 aattctttga tagcttcaca taagcgttca tcatagatat ggacttctga accaaataca
    27901 tcaaatgacg taaactcaac gcaatactcg tccatgacac cgatctggtt taactccatt
    27961 tcagataaat cacagaacgg cgtttcacgt cttgcacgtc tgccaagctc tcggtcatag
    28021 ttctttacag ttcgtcctac aaccttgcga gccagacaat caaattgaag tctgatagcg
    28081 ccctcgaaag aagatggttt cataatctca ccccctttcc agcgaaagtt gctaaagcga
    28141 aaagacttct acctcttttc gcactaacac tcaccgtgaa aggtgggatc tgatacccaa
    28201 ccacaaaaat tttttgaaaa agttttatgc agtaaaaaag cacaagggca atacagacgt
    28261 actgatcttg tgctaaacaa attattcctg ttatatgctg taaaaaacca catataggag
    28321 cattatgttc tgtagaattt aaatgttttt ttaatgaact accgatggtc gaaagttgtt
    28381 gaatatgtaa gtgtttcata cggctacccc tagagccgat aaaacaagac catccagtaa
    28441 tttgtccaac gttaggcaaa aataaaacgg cggctatata accgtcgtct tattcaaaag
    28501 ttcaaaaaga actaaactaa accctctaaa gcagtatgta tttataatca gtcttgcaaa
    28561 ataacaaagt agaattgaaa tattaaggta gcaatccttg tgcatctaat aaacttatag
    28621 gggttacagc agaccacgga ataatatttt gtatactttc agatcaatat agtccaacaa
    28681 aaccaattcc cgatatttag taactaaaaa gtaaactaga gtagaaaaca tttctttgca
    28741 aaacacaaat aataagtaat ggactcattg acaatagatt gagaaatcca gttttttaga
    28801 cttagataga ttatctcact tgtaaatgat ttaagttctt tgacttcata tatagcggta
    28861 taatctgtca acgtgatcat tacaaaccat aatgcctata taacatactt tccaagcaaa
    28921 aactttattc tattagttgg ggaatacaaa atatttatca ttgtgcgttc aacatattct
    28981 ttttgtataa aaatgtaatt acaaaacacc actcaatata agatagataa taatttgaat
    29041 aggaattatc aatctacgta accattcatt acctgtaatt gttgttacaa tagatttggg
    29101 attctgtttc cattacataa tttttttatc cttctttcag agatctcctc attttatcga
    29161 agcttcttct acagacatac attccataat aaaatctacc tttgaatatt atttcacgat
    29221 ttcgttatag acgcgtaaac aaacaataaa aaacgaaatt tcttttaagt accataattt
    29281 ttatatcgaa attacaatta tccacttata tacagcattt ataggtaatt caatgataaa
    29341 aaacacatac tttactacta aattgccaca attgaatgcg ccttgatatg atagggagat
    29401 tccacaataa aaaactgtgc atctcccctg attcattcat actgatatct tatttataag
    29461 actcttttgt gtcttttttt ggtttcatgg accaaaacac tacattcatt acaataacaa
    29521 tcagcaacac aactgccata gccacccaaa agccgatatt aagaccaagc cattcggtag
    29581 ttccaaaaag ggtcatccaa agttctttcc agttcatcat tccacctcct taaagcagtt
    29641 ctccgaaatg acggacatag gcttttttta aacttgttgc caaagtcata tacagcagaa
    29701 tacatggaat cagataagca aagtatgctg caggcaatgc aacaaatcca agcatagttc
    29761 caaacacagt aaacggaata atggtcagaa ctataattcc agtcattgtt agcaaagtca
    29821 ccggagctga tgcacgactc tgaacaaaag gcagctttgg agtacgaatc atgtgaatga
    29881 ctaatgtctg gctccacata gattccacga accaccctgc ttggaacatc ccgatatact
    29941 gtgcctgaag cattgccagt tctgcaccgc tgaaatgagc aggcaaatca ttaaataata
    30001 caccattcga tacgaacaat ggacaaaata caaagtacat aaaaatatag gttgtaaaat
    30061 caaagataga actggttggt ccaatccaga tcataaaact tccaacacta gaagcgtccc
    30121 atttacgagg tttggtaata aactcctcat ccacattatc ccaaggaatt gcagtacagg
    30181 ataaatcata aattaagttt agaaatataa gatgaacact catcattggt aagaaaggta
    30241 acaaagcaga tgcagcaagc actgagaaca tattgccgaa gttggatgaa gcagtcattt
    30301 taatgtactt gatcatgttg gcatatgtct ttcgtccctc aataataccc tgttccaaaa
    30361 ccatcagatc tttttccaat agaataatgt cagcagattc ttttgccacg tccacggcgg
    30421 tatccaccga aataccaata tcggcggctt tcatagctgc agcatcatta ataccgtcac
    30481 ccatgaaacc gaccgtatga ccattttcac ggagaacaga aaccacacgg gctttctgat
    30541 caggggttag ctttgcaaat acatctgtag actctgccac tcttgcaagc tctgcatcgc
    30601 ccatttgttc caaatcagaa ccgagaagca tattgcgaac ctctaatccc acctgcttgc
    30661 agatagttcg agtcactttg tcgttgtcac ctgtcaaaat tttggttata acaccatggt
    30721 ctttcagagc tttaatagca tccgcagtag attcttttgg aggatcaagg aatgccagat
    30781 atccaatcag taccatatca cactcatctt taacaccaaa tgcgccgatt ggggaaggat
    30841 tactcttctg tgcaatagct agtacgcgga atcccttatc gttaagattg tccactgtct
    30901 ccagaattct gtaacgaacg tcatcagtca gaggctgcac tcctccatta cactctgcaa
    30961 acgcacaaat agagagcatt tcttccacag caccttttgt taccatctgg gtcttaccct
    31021 tattatcctg taccactgta gtcagacgac gacgtgtgaa gtcaaaagga atctcatcta
    31081 ctttcacata gttttcagac aggtcaatga gacgcgaatc tgcggcttcc tcttcctcag
    31141 tcttatggat aattgccaaa tccatcaggt ttttatagcc agtctggaag taactattga
    31201 ggtaagcgtg acgcagtact cgcgtatcgt cctcgccatt tacattcaga tggtattcca
    31261 gcacaacctt atcctgggtc agcgtgccag tcttgtcagt acagagaata tcaatagcac
    31321 cgaagttctg aatggaattg aggtttttaa caattgtctg cttcttgctc atggaaacag
    31381 cgcctttcgc aagacaggtt gttacaatca tcggcaacat ttctggtgtc aaaccaacag
    31441 caatagaaac gccgaataag aaggccgcca accaatcgcc cttggtgata ccattgacaa
    31501 agaataccag cggaaccata atgagcataa aacgaatcaa tacccaagaa acggcattga
    31561 cacccttggt aaagcttgtt tcaaccgctt ctccagcaac tgcggacgcc atagagccaa
    31621 acagagtatg atcgccaaca caaacaacaa cagctgttgc gctaccagaa attacattac
    31681 tccccataaa agcaatgttt gtgtaatcag tcacgctttc tttctgagca cttacattgg
    31741 gtgttttttc aataggttca ctctcaccag taagacttgc ctgactgaca aacaaatcct
    31801 tcgcatccaa aatacgaaca tccgccggaa tcatatctcc agcagaaaga tgcacaatat
    31861 ctcccaccac caaatcatcc ataggaagtt cttttttttc atagtttcta cgagtaactg
    31921 tacaagtggt cgtaatcatg gctaacaatt tctctgctgc atttccgctt ctggattcct
    31981 gtacaaaacg aagcgtaccg gaaatgaata ccattgttaa aatgattaca acagtcaggg
    32041 aatcaaagtc ctctggcaca ctgccaaaca gagaaaaata cggaaaaatc atatctgtaa
    32101 ttgtggatac taaagccaga caaaataaaa tggcggtaaa tggattgata aatgcgccaa
    32161 ccagtcgttt tgccagagat ttcttttttt catgagtgac tttattggta ccatatttag
    32221 cacgacttac ggatacttca tctacttcta agccacgaag tgtggtatga agttttttta
    32281 aaacttcttt aataggattg gttgccgcaa attgaatgcg gcggttctgt tcgtcacgga
    32341 tgactgcttt ttctacagtc tgacgaatag ccatacgatt ttcttttttg ttcattggtc
    32401 ttacctcctt gaattttcat aggggcaagg aatattatat gagcgacacg gaatctccaa
    32461 attctcatat aatattctct gccttcgtgg actgacatca gagtccatgt ctttcacctc
    32521 acaatcttaa aaaaatatct atgtgaaacc tcaatgtgga ttattggttg cgatagcgga
    32581 cacttttttc gatataccag acatcaaagc cggcaacaat cagccatgcg attattgcaa
    32641 atacaaacca gtggtttgaa aatcccggca aaaatccttt cattaagaat ataatcccat
    32701 ttaacgcaat catttctcca acattacgac aaagcgggac aatattaatt ttatctttct
    32761 cctcctgcgg catatttttc cacagggaaa gatgacgata tccttttcca aaggccaacc
    32821 aaaagcctgc aagagtaaat aaaattccaa aaaagataca tgtaaaatcc atcgtttgtt
    32881 cctcctttca atgcttaaac ctgtttctta gttttgtcag cttcagaagt ttaatgaaga
    32941 gcacaataat agtaacagca agaataaacc agatcacaat actcacctga taccatatct
    33001 taaagattac cattctttgt tcaaagaaat tccaacccaa atagctacat aagattgtaa
    33061 atacagggta gaaagcaaaa agaattgctc gtttcaaatc ttcgatttct ctcctcactc
    33121 acaacacctc ccaaccagca ctaatcactt ccggaattac caatacctgc ccaacgattc
    33181 cttctaaaat attgtttttg gattttccga cagaacagta ttctgcgaca atctcaacct
    33241 tgtcaccaac cacatcaccg ctttccagat tgttcaaaaa gagcgtctta caggaattgc
    33301 tgttaatcag caataagcgt atatcctgct ctgccttttc ctgacatgta accgaaatac
    33361 gatactgctt ttcagattca tcactggaag taattgggtg aaactttctt gcaaagggac
    33421 gtagaatcag gttggaacca attaaaatag tagcggcggt gatcgccatt gcatacattc
    33481 ccgtacttgc aagaattcca atcgctgcag tacaccataa ggtggcagcg gtattcattc
    33541 cacgtactgt ccctctgtcc ttgaaaataa caccactgca taaaaaacca acaccggata
    33601 taatactgct tccgacacgg aatacctgat ccgaaccgta gagcatagga aacaaggtaa
    33661 agaaacttgt acccatgcag attaacacat tgatacgaat acctgcagga tgaccagtca
    33721 attgccgttc taatccaata agaaatccaa gtgttaagga gagggcaatt ctcaaaataa
    33781 aatctgtgta aaacatatga aacacctccc tttaactact aacagtatat aaaaaatcca
    33841 aatacaatcc tatggctaaa gtcttgcgat tttcttgcga tttggtaaga aaatagacgg
    33901 actgaaaccg aaaatggtct tagtccgtct ttgtcatatc tgatcattaa atttgtatcc
    33961 aataccccag atggtcaaaa tatatgtggg acaatccgga gcaggttcaa tcttttttcg
    34021 caacttccgg ataaaagcca taatgttgct gttatccaga atataatctt cctcccatac
    34081 ggcacgataa atctgttcct ttgtaaaaac ttcacctttg ttctggacaa gaaagtacaa
    34141 aatatcaaat tcctttggag tcaggttgac ttcagaacct tttatcagta cttttctgcc
    34201 tcttggataa atttccagat cccctgccag gattctatct gacagagtac ccactggcca
    34261 atcgctttct tctgatgctc ctaacaataa cgaagatacc tctccacgaa tcatctcctg
    34321 aataccagaa agtaaatcct tcgcacaatc actgtccggt ggatctctta gtagtttttc
    34381 taatagccag atttcaagag ccgaatccat cggaatataa ctaatattct ttttcatcat
    34441 gaatgcctcc tcttaaatca actcttgata ttttttctta taaaaatatt tggctactga
    34501 attcagcagc atatagagag ataccaccag cagtaaaaat acgaaatacc aaagaggtaa
    34561 tttcgtcagt ccaaacaacg acgcaccgcc gacaaatgta agggaagtaa aagcaataat
    34621 ccccactaga gttatagaaa tgaccggtgc agatgccctg ctctggaaaa aaggaatctt
    34681 ggaagttcgc agaaaatgta aaatcaaaac ctgtgtccac atagattcca aaaaccatcc
    34741 cgtctgaaac agtgctacat actgaagttg cagggaagga tctgtaagct gcagataggt
    34801 tgcccctcca catagcatcg gacataggaa ataatacaaa aataggaagg tgacaatatc
    34861 aaacagagaa ctgatcggtc cgaaagacag cataaactgt ctcagtgttt tgccggacca
    34921 gtctcttggg gacagcatct cttcctcatc tacattgtcc caaggaagca caatacacaa
    34981 cgtatcatac agcagattca aaagcagaat ctgaattgaa gtcataggca ggaacggtaa
    35041 aaaggcactg gcacaaatga tggaaaaaat atttccaaag ttagagctag cggtgatttt
    35101 aatatatttc agcatattgg taaaagtttt tcgcccctcc attacacctt gctccaaaac
    35161 attcaagtcc ttttgcagca aaaccacatc ggcagcatct tttgctgcgt ccactgccgt
    35221 atctacagag attcccacat ttgcttcatt cagtgccgga atgtcgttga ctccatcacc
    35281 aagaaatccc acggaatgac cattatcctg catagcagag acaagacgca ccttatgtcc
    35341 cggagtaagc tctgcaaaaa catgagtatg ttccacagcc tttttaagtt cacagtctgt
    35401 catttcatcc agttgagtac cagttaagat acgctctgta gaaataccca cccggcggca
    35461 aacagacaca gcaatcgctg cctggtcccc tgtcagaatc ttagggatca cttttaatct
    35521 tttgagtgca gttacagact cctttgctgt ctgctttggt gcatcaaaga aggatagata
    35581 gccaactaga gtcatgcccc attcgtcatc aggagttatt ttcttttgcg ctcccacatt
    35641 ttttcgtgcc accgcaatga ccttcattcc gtcctgtaac atttcaccta ctacagaaga
    35701 tacactatgt ctcgcatcct gccccatggg gagaagtttt ccacgatatt ctacatggtt
    35761 gcagcgtgag aatatatgtt caatatcacc tttcataata agctgactat tcccattgct
    35821 atcctgtaca agtgtactga caaacttacg agcatagtca aagggaattt catcagcttt
    35881 ttggaatttt gtcagaagat ttgcgtaatg ggcttcccgc cctggcatgg tattgcaggc
    35941 aagaatagca ttatcaatgg tattgcagac accagaatga tagcagctat tcagataagc
    36001 aagatcaaga acctctgcat tttcatttcc aaaaatatcc atataatatt ccagcaggat
    36061 gctttcattg gtaagtgttc ctgttttatc catacaaagc acatccatgc taccaaagct
    36121 ctgcatggca ttaatatcct tgataatggt ctgctttttg ctcatagcaa gacttccttt
    36181 ggcaaggcag gctgtaataa ccataggaag catttccggc atcagaccta cagcaactga
    36241 cagggcgaaa gcaaaggatt ccatccattt tccatcagta atccccagta atataaatac
    36301 aatgggaact aatatcgcca taaaacgtat catcacccag gcgatggaat cggctccttt
    36361 tttaaaggaa tttttatcat cagaatccgg tttggtaaat cctccataca gggtatcttt
    36421 gcctactgcc agtacaatgc cttccccttt tccactgatg actgtggttg ccataaaagc
    36481 gaggttttca agctgagtca gtgtatcctg ctctccgtaa ctgagttttc ggcagctctt
    36541 ttcaatgatg gcgctctcac cagtaatcgc agcctgagag ataaagagat ccgtaatctt
    36601 cgtcaatctg atatccgccg gaacacgatc acctgcagag aatagtacaa tatcccctac
    36661 cactaattct tcgccaggaa tctctattaa ctttccatca cgcctcactg tgacgctttc
    36721 atgaactagg cgatcaagct gagcagcagc gctctttgct cgcagctcct gaatcagacg
    36781 aattacgcca ctaattacga tcatggaaaa gatgataatg gcggtagtag catttctcgc
    36841 aaaattggat gctacgaaaa catccgtaac aagagaaaca atgcccagaa caaaaagaat
    36901 cacatgaaac gggttaataa atgcccgtct caaccgttgc atcgtcgtat catttttccg
    36961 cccattgaag ctgttcgctc cgtatttctc ccgcattgaa ccgatttgct caatggataa
    37021 accatccgga gatgagccaa tgtctcgata aatttctgat gttctacagt atgcgtattt
    37081 tttgatacga ctatcaaata gtgttgcttt tgacacgaaa catcccctcc tttataagta
    37141 taagtaataa cattttaaca cagtgctctg aaaattctat atttatattc ttgctttttt
    37201 cttgcaattt actttgatat ggcatttttc agctcgatca aatccgtctc gttagggtga
    37261 gataaatcct tatcaaagtt ttcaaccatt ccttctaaat ttggagcacg gatggatacg
    37321 gcaagcattt tctcatagcg ctctcgcaca taaataggca tttccccttg acacatatga
    37381 gtgctgacaa acttcttctt ggttttcacc ttgaccatgt atcaaatttc caaataacta
    37441 cttagaaaag ttgctcataa taatatttgt attatgagtg atagaatgac aaaagtggca
    37501 gtatggaaaa cgagtgtaaa aacaatacaa ttagtttgta tggaatatct gaactacata
    37561 attgaccact ctgtaattta tgaaaaaata caatgcgcaa attggaacgg gacagaagaa
    37621 accaatagta tttttaataa ttccccttca ccattgatta ctcttatcta ttcttgcgat
    37681 ttacaacaaa ataaaacaac ataaatttta aatacgttga ctggcataat tcatatgtat
    37741 agtaagtcta caattaagat gcgctttttc taatccaaat atattaagaa aaaaattttg
    37801 cgaagccatt aatactttgt ttaagatgtc tttcataaat ataaagaata atgctcttaa
    37861 caaaagcgtg caaattccta aagaaatgca cgctttcaat aattatccta taatcttcca
    37921 gttgctatcc ttatgtagca ctaattcaaa ctgcgatacc tgtgttgcct ttgtctgatt
    37981 atcaatgaat ttcacggcaa ccttgacttt cacattgtcc ccatcaaccg taaagatagg
    38041 gtttaccaat tcagaataga ggtagtccct gccgatagac tcaagcacat ttccagacac
    38101 atagtaagca agctcctttt ctgtggctgt tgggtatagc ttaaagaaag tttccagaaa
    38161 tgcagtagcg tcatttatgg tatcagcgtc tacacttgcg tctgcttctg gtgtctttgg
    38221 ttcatagtct gatttctcga ttgctggtgc aagggtaggg ttctgaataa ttaccatatt
    38281 cccgtcatcg tccacatgaa cttttacggt ataggttgcc ttaaccgctg ttgtctgttc
    38341 cccctcttct atctgctgat ctacttcgta ggtagcagaa aaagtgtccg ttccagactg
    38401 ttcaatgctc catacaatca catctgtaat tgtggagctg gtcggaatat cggttctaat
    38461 ggtatccaca ttcaagtcct gcaattcctt tgtcagataa ccgttgattg cctgcgtcct
    38521 tgcttcgata gcttctttgc tgttactcca tgtataataa gactttgcaa agttcttcac
    38581 gaaattttct atcccgttgg tgtcctgcaa gcgaagctct atcacttctt tttcatgggt
    38641 cgtatgctgg tcgatagccg taaaattctt atacacccca aagctcacgc tttcgataag
    38701 cactacccac aacgcaatca cggttttctt atgtgtgcct accttgacag tacgcacctt
    38761 tttttctttg acattttctg tcgatttctt attcttctta aacatatttt caagtccttt
    38821 ctattctttt actcgtcctg caccgattaa gtgctgttgc cagtaactac tacttaggtc
    38881 tgcatatcct atcgggtcgc ctgcatggta cattttgtta tttcctacat aaatgccgac
    38941 gtgtgttaca taacttccag cgttataggt ggaatggaaa aacaccaagt ccccagcctt
    39001 tgcctgcgaa agaggaagat gttgggttgc gtcatactgg gcttgtgccg ttctcggtaa
    39061 ggagatacca gcttttccat agcaccattg cacaagtccg ctacaatcaa aggaagtgtt
    39121 cgggttacta ccaccataca catacttcca gccttgatat ttcaacgctt cattcataat
    39181 cgcttgtgcc gtcgcattat caaagtgtgc cacggtcaga tattgattga ctaattccac
    39241 atagaacata tttccatagc cataccgcca gcccccgttc ttcgcaacag ctatcgggtt
    39301 ggtgtaggtt actttctttc caaccgattt ctcacgtgcg aagctctctg caaggttaaa
    39361 ggtgtgtttc tttcctttgc ctgccacata tcctacatag ccaccgccat agttatagga
    39421 ttgtatcgct acattcaaat cgtcgatacc ttgatttttg caggaagaaa gcagggacgc
    39481 aaaatactta cacccctgct tgattgagct ttccgtatct aaagagttgg gcagtagtcc
    39541 cagactttcc gaactctgca taacatcttc tgccgtaccg ccactttcta cttgaatgat
    39601 agccagcaac acatggacgt actcggaaat gccattttct ctggcgtact tttctaccat
    39661 aggctgatgt ttcaagactt ctgcggataa gttcataccc gtaatgccag aagaaaaatt
    39721 gctgttctcg tcgtcgctgt ccgcactaat caagacaccc aaaaaaagca ccagagaaaa
    39781 gaggatagga aacagactgc caatgatagc gatatgtttc agtttcagtt tcattttttc
    39841 ttctgtcctt tcttcgttac aatgttacgg tttttctctg tggtctgctg gttcttttgg
    39901 acggtttggg tctgctgaac ctttgtcctg cggtctttgg tgtaattctg gcgtgtttcc
    39961 tgcgatacca ctttttctac attctgccta tgtactggct gtgccgactg ggtcgctttc
    40021 atatcagaag caccagaaga taacggacgc tctttgataa catttgtctt gactggctca
    40081 cttgctttag aagtcggagc tgtggcaggg cgtttgattt cctgcatttt ttcaacactc
    40141 gtctttggaa cggttgaagc tgtggctggt cgctcatggg gacgggtagc tcccgttgtc
    40201 gctgatccgt ccgtttttct atgccttgct tcttgtgcct tttgaagctc catacgcttg
    40261 acagcgatac ttttcttatg ctgttcctgc ttttccaagc gtcccgtctg tctggactgc
    40321 tgttcctgca ccataccacg cttgaaatca gacacgctgg actttgcctt ttcctttgcg
    40381 gaatacaccg cataagcggt ctgtgtgggc atatccttga tattctcttt gacagcgttt
    40441 gccttgtctt tcactttatt tttcgtatct aagacagcac ccacctttga gcctgcacgc
    40501 tgtcccatac tggaagtggt attgccccga ttttctttag aagccgtatt ttttctttcg
    40561 gatctcttgc cagcaacggc acttccagct accgcaccag ccacaccgcc cgaaataccg
    40621 ccagcactta cagcccttgc aatacggtgt tccatacgcc tagcccgatg tcgcatgaac
    40681 agatacggtc tgcggaatat cctgcgtccc atgctttgac tgtcgccagc gttgagagaa
    40741 aacatactca ttaagtcgcc cagcttcata tagataccag cgaaacatac tatctgcaag
    40801 aacgccacca tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgcta
    40861 aacgcaaccg ttataatgag cgttattcct gcccgtgtca ttatggtatt aaataccctc
    40921 acgattgcct gctttgccat gctttcatag ctcggtatca tggaaagtag aaagctgata
    40981 ggtaaaaaca tagcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc
    41041 aagaatacaa atatcgtaat accaagatta aagaacaaga ggaagaacac catacctaaa
    41101 cggtttacca cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc
    41161 acgacttctt ctctggtctt tccgtcctcg tcctctggac ttgccgatac gagagcttcg
    41221 acacggtctg tcccgatttc ctctgcgttg ctgtttccaa actgtaagag tagccacggc
    41281 tgttgtactt gaatagaaaa taggctgtcc cgtatcaagt ctacgctgtc cttgccctcg
    41341 ctgtcagagt tggggagcat gatttttgtt cccaaatcaa gcgaagcggt actgatgtct
    41401 gatgaaaatt catttatctt cttgatgtag tcgggagcgt aggcaataaa cgaagcggac
    41461 aacacgaaca ccaccacaaa gttgataaca gcgtgaagtg ccttgctggt ttcccgtttt
    41521 atcagtcccg tataggcaac atacattccc accactaaga taataagaag caggaaacca
    41581 acatagaagc ccgtagaaga aaagccgttc tcggtaacgc ctgcaagggt ctgtatgctt
    41641 tttccgatac tgtctgccat atcgttaata aaatcaagtt tataggcttc ctgcaccaca
    41701 tagccagttg cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa
    41761 agcccgtact gtaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag
    41821 ctattatcga cgaaaaaatc aagctggtag ttggaaagcg gatattttga atatagattt
    41881 tctgcgttta tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa
    41941 agcaggataa gggaaagtgc caccacgaac agagccattt tgagaaagtg aagtatcttc
    42001 ctttttgtga acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtacgg
    42061 gcggtctagt atcaaaggcg tgtagtaatt cttcaaagac tgggtgtatc tgcaccacac
    42121 cgacacgccc gtataagtcc tgcaataaac attgtccgtt ctccaaatca cgcaagcgtt
    42181 tctggttgtt ttcgtcgtcc ttgtcgatac cgaaaaattc taaggtctgc tttatctcgt
    42241 tgatgtcagt agaacgaaat gcaaacttca aaccgatatt gtttttcaga ctttcctttg
    42301 acacgtcata ggcagactgg gtaacgaaat aaacgcctgc ctgcatagct cgtccagcac
    42361 gaaccagctt atttgatagg gtttcgcctt gtgccacatt taagaacgcc cacgcttcgt
    42421 ccaaatctac aatcttaaaa atgcttctgt ccgaatggat aaaatcaagg gcaaaggtac
    42481 taatcacaat cagcatagac accgacaata attcaatggt cgtgtattcc tcaaaagtgg
    42541 tatctttatc tggcagtaca aggtctgcta cttgaatgat attgagctgg ttatcaaggc
    42601 ttatagcatt ttctaccgtc ccgtctgaaa acagcaggtg tgcaaagtcg tagtccgtga
    42661 agctgtcgat atggtctgcg atattgcgtg agatagcggt atcttctttt ctcaattcct
    42721 caataacgtg taacagtcct ctattttcac tctgggtaac agaacgcacc gcttttcgta
    42781 acacggggaa tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga
    42841 ttgccagact ttcagcgtct ttcacattct tcataatcac aaatgggtca agaagtcctg
    42901 cattgtcctt gtcgctggta aggtttacga tattgatttc atgggctatc tctgggagcg
    42961 tttctttcca gttgccacgc tcacttttag ggtctaacag aagtgcttga ccgccaaaca
    43021 gcaccgcata atatacaagt agattgttgc agaatgactt tccaccgcca agacttccca
    43081 caaaagcaga agcaagggca ttagtaactg tgccttttac tccctgcgaa gctaaagacg
    43141 gttgcaggta cacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt
    43201 cccctaactg ctgggtcgca ccaaaaccaa gcccagccaa gaagtctgac tttacatact
    43261 gcacatagtc attgatatat cgcttgctgg caggtaaaaa ttcagaatga agtcccaaca
    43321 tatcgccagc aggacgcacc agttttacat tgaggtcgtc gtaaaagtcc ttgacttcat
    43381 cacaacggcg tttcagctcg tcaagatcgg gtgcagacac ccgtatgacg tagcttaact
    43441 tatacatact ttctttgctc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag
    43501 cgtccaccac atttgagctg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt
    43561 tcaattcctt tttcttgttg cggactgtcg ttaatgcctt acggttctcc acgatttcta
    43621 cattcataga agtatcaacg gggaatgtga attgctgttg ctggaaatag aagatttcag
    43681 aggacggaaa atccaactcc ccgacaatta cattgacggt aaaataggac acatagcttt
    43741 ccttgtcctc atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg
    43801 gacggataag gtcgtagtat tttatcagcg tttctttctt caatttcttc tttggtagct
    43861 ggtactcata atcttcatag gcgataccgt ccctgccgta aagatgttcc atgagatacc
    43921 caaaatcatg gatttccaag cgacgcacct taaagcgacg ggagatttta ttttccagta
    43981 acttttccat tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca
    44041 tcagcgtgtg gttcacttca tgaagaaatt ccttgaacgt cagccatgcc gattttttga
    44101 tgttcttcag attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt
    44161 agtccacttg attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatttttt
    44221 ggactgccac ctcacgaagt ttgcctgtta ccagtttctt tgactgctcc tgcatacttc
    44281 taatggagct ttctgtggca atctgcaacg catgaatttt tccctcacgg gactgtgcga
    44341 ttagctgtcg aaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt
    44401 tgtacggtat cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttat
    44461 tgtcaatata ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc
    44521 aatatctgct tatgcagttt tacggctttt cctgcataag tgattttagg tcgcaaggca
    44581 taggttatct gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac
    44641 ataaaccaag tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg
    44701 gaaagtgggg gcatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta
    44761 atctgtgtaa aggtaacggg aaagggtaag ttaaagtcat tgattgcata taagactttt
    44821 tccacgttcc agatacccgt gtagctttta atctttttca agttctttca gcccctttcg
    44881 taagttcttc aaagagaaaa ggacagccat tttcagactg tcccttaaag agaaatacgc
    44941 tgtcagtagc aacaatgctt gttgctgatc ttccagcgtt aatatggtat ctcacagata
    45001 cctcggcttg tttcaatgaa tcgaccaccc atatccaaat ctcgcccgta ggcttcatag
    45061 tcaatatagt tctgtaagtt agctggaatg tccccaagtg cgttcaattc gtctatgtag
    45121 tagtaggcaa catctgtcat ggtttcacaa tcgggataaa aataaatatc gtccttatgt
    45181 tccacgactt cttccagcgt cccatagtgg ctgataaatt cgtccagaca ctctacgata
    45241 tagtcgggaa gttcctctat catttcatac atttcattga gttcttcaat ggaaacatat
    45301 tcccctatgt cgataggaaa gttgtcggta tcatggacag cgtattcttc gtactgttca
    45361 ttcaagccga ttttttcttt tacatcttcc tcgtcaatag ggaaagtgaa ccacgcaccg
    45421 actaaatatc cctcattgta tctgccaaga ttagcaatat aaaccgccat atcatcaatc
    45481 ataagcacca cctcatttcc tactctggca gttcaaagat accgtgttcc gttactaaaa
    45541 attttccgtg ttcctgcaag tgagaagcat aggcttcaaa gtcaaaatag tattcttgac
    45601 agtcctcgga taaatgtttg aatgtcgggt cgttcatcag cttttgcctt gcaacatcaa
    45661 tcatgctttt gcagtcggga taaacggtaa tcacgttcct gcaaatataa agtgcttcta
    45721 aattctcata cacggtaagt agtgatacat attcttcctg catatcactt gaaagctggc
    45781 ggtacataaa atctaattcg ttgagctgat acacgctggt gtgttcgtgg acttcatcag
    45841 cataaggcag taccttttcg ataatgcggt aatccccact ttctgcaccg acacctaatt
    45901 tttcttcaaa ctcggcaaca tctatcggca ggtcaaacca gtatgatatt gtttcttcgc
    45961 cagttgttcc tctcgtttcc accagcaccc ttgtttcctg cattgttcct cacgtccttt
    46021 ctgttgtttc ctcacatctt ttatggtctg gtacgacata ccaaacacat actttgaaaa
    46081 atcttctaac tgtttttctt cataatcggc agggttcagt cgtttcattt catgccacac
    46141 tttacgcttg taagagggca ggtcggaaat gtattcacag ccgacttttg cctgcatatc
    46201 cttaaaaata tcgttcattt catcactcca atctgaaatt ttggcaagaa aaaagcagtc
    46261 aatcctaaga ctaactgctt tgtgtatatc tggtattaga ttatgaaatt gagtttatca
    46321 attcttgtac ttcattaaaa aatctggata aatgaaaacc atctgcaacg gcatgatgta
    46381 tattcattgt aagaggcatt atatatcttt ctcgctctaa ttcgtattta ccccaagtaa
    46441 taactggtgc aagaaatttc ccttcgtcaa aaacatgaac atcaaaatga ttgtatttta
    46501 cccatggcaa acaagataca tcaaaaaagt tcaaaggttg atttgctacg aatgcacgtt
    46561 ccattttatg tttctctcta tctaacataa atcgttcatg aaattcaaat atgctatcag
    46621 aaaaatctgt aaccattttt gtgaaattct catcttcttt atgaaattca gcataggacg
    46681 gggaaatata gtcccattta attaaattac ctttattgtc ccaactatat ttaaactcat
    46741 catgttcatt aacgaccttt gaaacaaccc atatcatagc tggataaaat ttcaaatcat
    46801 ggctttttga aaattcaatt agatttgtta catcaatatc tacggttaag ctcataacga
    46861 tacgcatatc atcaatgtaa ctttgaaaca atttacctct attccatttt tctaaatcaa
    46921 tttttgtata tttatattcc atgtccattc tcctttacat tttgaaattc agtaaaatgc
    46981 aaggctctct ggataacatt tcactccatg caagtctatt ccattaagcc ttgtatggag
    47041 ataatacaat acacaatttc ataatatccc tccttatcca ataaacctta gtttattgtt
    47101 ttcatactca gattatacca ttcatatata ggctctacaa taaaaatcat tatgcaccaa
    47161 taatcttatt gaacagctct aacagtacat ctttcacacc gccagcgttg aatactaagc
    47221 cgacagcaat aagggcaact accaagaagc caataagttt tgaaaactca cgcttgaacc
    47281 ctaagtagat accgataacc acaatcgcca taagcactaa gctctgtgca tttgataaaa
    47341 accattgata caaattttgt ccgaaattca tttaattgtc ctcgctttct tctaattgtt
    47401 tcatttcata gtcagcttct ttgccgatac tcaaaataca catcaagact acgccgatac
    47461 ccattcccat agataccagc aggaagtctt ttactaattc ccacattttt tatcactcct
    47521 aactttccac caaatctttt gcaggggtcg tctgctgttt gattatcatt tcatgctttt
    47581 cactcaaagt tgcctgcttt tcaatcgttt ccatatagtc agtcccgttt cccttatcaa
    47641 tctttttcag cattttcaag gtaggtgcta cctgcctttg tacccaccgc aatgtacggt
    47701 ctaaggtgta aggctctggc tttgtcgtca gcttcaagct ctgtctgtta tcgccgataa
    47761 accaagccca gcggtcattg agtttccagt cattttttcg cttgtctggt tcttcatcaa
    47821 caaaccgcac atactggttg atgatagaaa aggcggtctg ttctgcgtca taataggtca
    47881 gcaaatctcg tactgcataa taggcacgct catttctaag ccgtatctca aaacggttga
    47941 taatgtcggc ttcattaagc ggtgtaccca acttgacgta ctgctcatag tccttttcat
    48001 agatacagaa atatacatct gatttcagcg aaccaagata aagagtgcgt cccatatatt
    48061 ctctgtcgtc ctctctgtgc ttgataagct cgcccgactg gtaaaactta taacttctgg
    48121 actttccgat atattcccgt ttcctgcatt tttccgcaag ctctggaata tccaaaatgc
    48181 ccgtatggtc gttgatagca aggtcgatac gcttcatcac gccaccgtct acgagtgcgt
    48241 ccatgaggaa gtcataccag cttctttgct gtgccaacag gtaactttca aactgcctgc
    48301 aaccacgccc ctttaactct aaaaggacac ctttttcttc gtcagccgac gtatagataa
    48361 agatgtcccc taaagagtaa tgctccgtat agctgtaatg tccgtaatct tcatggagca
    48421 tatagttgat attcagtttt aatatatctt tgatgatgtg ctgtatatcc agcgtgggaa
    48481 aacgaatttt tacataatca aacaacatgg taagcggtgc ttctggattg aaccttgcca
    48541 gttccttatg cagagtttcc agaagttcct ttgacggctt catttttccg ctttctatct
    48601 tattgagata ttcccttgtt ataccagaag ccaccgccag cctgccttgt gagataccgt
    48661 aagcaatccg tttctcccgt aattctttta tccattgttc ttcattcaga accatacccc
    48721 caatctgtaa agtgtgaagt ttttaggggc gacttcacaa ccgatttttg ctaggaaacc
    48781 atgccagaag ccttatgttt cctatgtttt ttgtctattg tctatttgca aacgtacccc
    48841 tctgttagat accgaggggt tttacctgct ggcgtggctt acgccacacc agcaacggct
    48901 agtccgtgcc gtcgcctttc gcttcgcacg tcgccgtccc gtcctgcctt gcttgtgcaa
    48961 gagaaccgat agtcttcaaa aaatcatgtc ctttcgggac taagggcgtg taaaattcgc
    49021 ttatcacgct tgttcctaca tcacaatagc cacgaccctt gatacgcttc tggaaaaact
    49081 gctttttcac atcagaaccg aacagcatac catatcctaa ctcgctgata cgccccaatc
    49141 ctacacggaa attgaagtta tctctgatac cgtccgagaa atactttgcg tctggacgct
    49201 ggcaggcaac gataaggaaa taacctgctt gtcgccccag cataacgatt ttctttaact
    49261 ggctaagtag gctcacgctt tctttcgttc ccagcatttc aaaaaaagcc acatattcat
    49321 caaagataag aaagcagggt ggcagtccca gataggcgta gttttcgcct gtcttgtagt
    49381 tcgagtgttg cttcatttcc tcacttcgtt gtaccatgcc ctcataaaag gaattgacgc
    49441 aatctatcat ttcttctttg gtgtggtaca cgtttcccat gactgttccc aaatctgcaa
    49501 ggtcagcgtt ctttgggtct aagatgtaaa ggacagcgtt ggtatgcagt aaggcttcaa
    49561 tgagcgtcag cagaaagtaa gtcttaccgc caccagtccc accagcaatc agggcgtgag
    49621 ggagtgcgtc atattcccag acaagatttt tcatcagtct aagacagccg ttttctgccc
    49681 gtacttcatc aatggtaatg cggtttgcta tcatatcata aagcagggta tattcaatat
    49741 aaccgtcatg cagtgttttg tcggttagct cacaatacaa gccactttcc aatttatcct
    49801 ctaaccgtaa aagctggtct tgatatttcc ccagcgtaat ttcacagcgg atatgaagca
    49861 gtcccttttc catttgataa taaatctttg gaaaccagac gattttttcc cttgatctgc
    49921 tttgcaggtc agtaaaaaaa ccgctgtctt gtacggtatc ggcttcatac cacttatttt
    49981 ccagtatcat tcttgccagc ttttgacggt gcaggagctt cttgaaactg tcgtaacaga
    50041 agcgataata cagaaaagcg accaatgcac aaacaccagt cgctatcagt atggttatca
    50101 agttgtaagg ggataacgtc aagccttttt ccaacaagct gaaatgttcc caatcggtac
    50161 gcatgagctg tttgatattc agtagcagga gaaccgccac gaatacaaac agaagcgtcc
    50221 ctatggaaaa gtgatagaca aggtgcttgt cgctggctct gatacggtgt ccttttttcc
    50281 aaatcttacg cataaatcaa tcactccttt ctgggtacga aaaaagcagt caatcctaag
    50341 actaactgct agttacgatt tatttgaaat gtgaaatctt tccgtatttt tcgtatgaat
    50401 atttaagcat ttgataaata tcttctctaa gaatatactg gtatgtttct gagattgttt
    50461 catacaccgg aataggaatt gcttttgatg aagtcctttg gttcgatttt atcaacactc
    50521 tttttgctgg cttagatact gttttatgaa cccaatccga attcaataaa cagtgtccca
    50581 taggtgtacc agaactgcaa tatggttgat tttcattctt aataaatcta agcaataatt
    50641 tataatcact tgaagataag ttatccacat ctgtccaaag aaattctaaa ctctgttgcc
    50701 attcttctgc ttgtctctgt ttgttacgtt gttccacacc ttttgaataa ttatgatact
    50761 gtattttctg taccaaccat attactaatt cgatgattag aaaccatacg cagaatagga
    50821 aaacagcata cccccaaata gtgaatttta ccaataactt attatccgta ggtgtaacgt
    50881 aataagctat aaaagaaaaa actattgcaa taactgtcgg tactaaatac ttttcaagaa
    50941 atatctttaa taaatctaat aacttttcac tcatgcatct atgcccctta attaaaaaat
    51001 cacattaaaa atattatacg atttcttaat tgcagaggca atcaaaatat atttgatgaa
    51061 ctaattattt tttcgtttgt ccctgcggtg tatggttctg gggattgcct gcgttttgat
    51121 tgcctttatt cttcaacaca atatcctctg cctttacata ccagtccaca tctgcacccg
    51181 tataggtctt tcttgatact gtatcggcta cgggattgac aagctccaca actgcattgt
    51241 acggaaattc ccttaacggt acttctggcg gtacggacac ggggataatt ccaccatgca
    51301 gactgcactt caaatcatag atacgctttt taagctgagt actcggtgtc ccgtcctcat
    51361 tctgcaagaa tacatcacgc accgccgtaa attttaattc tccaaatgtt ttctctttgt
    51421 caataacaaa cccgtttgat aatctcataa attcttcact cctttacttt tcttcaactg
    51481 ctacaatatc atcagcaact aacacataat cggtatgtcc catatccccg attgcgtaac
    51541 ctctgccgta taacttcgga ttgacaagtt ttactttctg ctcatacttg aaatgctttt
    51601 caccagcctg cacgggaatt tctaccacaa cattttcacc tttctgtacg tcagaataaa
    51661 ggttatagct tcgtctggct aaaaccctgc ggttattttt atctctttca aagactggct
    51721 cactttcgcc tgcaaattca agagttccaa aagactgtgt catatctggc acgacatatt
    51781 tcatttccat agtgttttac ctcgtttctt tctatttttg ataagttgat tgattgtgat
    51841 ttcttattct ggcggttggg taagtaccag caatccccca gatagtaaag ggtaaaatca
    51901 atgcttacgc acccttgact atccgtgttc ttgcaggttg tagacagaca agccagaata
    51961 agcggaagtc taccgtttga ccgcttcggc ggagaacgtg atttttcttt catcataccg
    52021 ttaccgcctt atgatttctt cattttacgg cgtttgtaaa gatatgttcc agccagtcca
    52081 ccagcagacg caaacaacat cacgataaat gccattacat ttgtactgtc gcccgttttg
    52141 ggactgtcgc taggtctgtt tggcttctct ggtgtctgtg gtgtttcggg tttctctggt
    52201 tcttctggct tttccttaaa ggtaatcgtc tgtccctcgt cctcaatgtc tttatgctcg
    52261 gtaactttct tcggcttgtc tggattgctt aaatcgtata attcctcaaa agttacaagc
    52321 tgtttgccgt caagggaagt agcgtcaaag gtaaaggcaa cttccacttt catagtttcg
    52381 ctgtcagcgg taaaggtata atcactttcc acacgttttc cattgataag aagctctgca
    52441 ttttctgctt tcaacatctg ccagcctaca agtttatact ttgtaccgac ttctaagccc
    52501 tttaaggtta cggtatcaat gattgtaacg tcttttcctg cttcaagctc tttgttgccg
    52561 tccttgtccg tagcagttgt atgaattttg atgatacgct ctgtgattaa aacagtttgc
    52621 ccgtcgtcct caatgtcctt atgttccgca actttcacgg gttcgtctgg attgctcaaa
    52681 tcatacaatt cttcaaaggt aacagggttc ttgccaccta aagcagacgc attgaatgta
    52741 taggaaattt ccactttcat ttcttcctca tcagcgacaa aggtataatc attttctaca
    52801 cgtttcccgt caatgataag ctcggcgttt tcttccttta acatctgcca gcctttgagc
    52861 tgatattttg tgccttttgt aagtccgtct aatttgacag tatcaacaat cgtaacctct
    52921 tttcctgcaa ggatagtctt ttcgccgtcc ttgctggttg ctgtggtatg gatagagatt
    52981 tctttttcgt attcatcagt caaagtccct aaatcaatca caagattatt tcttgatacc
    53041 acgatttcaa aaggtgggat aagctcaaag cctttgttac tatcacaaca catttcttca
    53101 attatgtagg tatcataagg taacgcaccc ttgctgtcgt ctggttcaga agtcccaaac
    53161 catacaccgt cctcgctggt ctttcctgcg ttggtattgt gcttgtgaga agcccagtca
    53221 gcagaagtgg agaattgccc gttatcgtca gttactacga catgactttc tcccgtcgtc
    53281 ttgcttgtga tccgaaacgg aacatcagca agacgcttgt gtgtgcctgc accgattttt
    53341 acgccctcaa tatctccacg cttaatctga ttgtagatag aatgggcttc gtcggttaag
    53401 tccacgattt ttccattttc tgtgattgca aaatcaatcg gttttgcacc gtctgtcaaa
    53461 tatccgtcgg gtgcttcact ctcaacgata cggaagtttc cataaggtaa aaggtcagca
    53521 gaagtagaag cgacaccctc aatatctgta cgaatagttt ttaccacttc atttttcttg
    53581 tataacttgc cttcaaccaa tactgcatta tcatttaagg aaatgatgtc aaaggcagta
    53641 tctttcaagg tagcacttcc ttgtggtttc gtatcgcctg tttctaaatc tcgtttctgg
    53701 attttcacac cgccacggat aaccttgtct gatacggaaa actggttact tccagtcaat
    53761 acggcaaggt cgccgtcctc agtaatctgt gttacatata cgccctttat ctgttcggat
    53821 ttatcgccag cctgcatata tgcaccgtca agtaagtagc cttttggagc ttttgtttcc
    53881 tcaacggtta gtgttcctaa aggaagaacc gccttgccgt cctgcatata gaaactgtcg
    53941 ccagatacct tgtatgcgtt cgctaatttt gtgatgtagt gggttgtctc gtcgctgtca
    54001 gtttcagcga ttgtctttgt aacccatgta cgagtagctt cggcagggag attgtctttg
    54061 ttatagaagc ctgcataata cttccatgta aatttcgcac ctgctagaga agcgttccct
    54121 tgcggattgt ctttctgtgt ttccatatca atcttgaaaa gctcaatcaa agtatccgtt
    54181 acctttggtg tatcagatac ttttaaggtt gctgtttcgc cagctttgat tgtcaatgga
    54241 tagactgttt tatccacttt atatcctgct ggtgcagaaa gctctttgat atatactgtc
    54301 cctgctttga cttctacaac attcgtattt ccgttttcat cagtcgtaag ggtggcaagc
    54361 tgttttgtgc agtccttatc agaaaagaca ccataagttg caccagagat agagtaattt
    54421 ccgttaccgt ctgtaatgct ggtattactg gaagtctttt gcagcttcgc atttccgaca
    54481 ttcagcttcg cccagaattg tcccaattcc tgcccctcgc cagagtagat ataaccgcca
    54541 cattcatagc gtcctttatt ttctttgaca aaggcttttg caccagcgaa aacttcgtcc
    54601 tgcgtagctt ttggtatttc atcataagaa gctcgcacgt tgtcgcattg ccagccaaga
    54661 tgtacgctta atctctgcca gacaacacat tgttccaaca gataaacttg tttgtaattc
    54721 aattccttgt gagcttcgcc atactgtttg acatactcta aggataacgc cacatctgaa
    54781 atctggtcgg cactcatgcg tgagcttgcg tcagttctgg tcttgtaacc attcttgaaa
    54841 tctgtattga tgtcaataca ataggcagtt tcgccctcaa ctttcatcat gccctcattg
    54901 aatgtagaac cgatagaacc gtcattcatt actttttcaa cgataccgac acgctctttt
    54961 gactccgtcc agtattgctt gctttctgca tgaacgggtg tagtcggtaa agcagtaacg
    55021 atagttgcaa gagctaagaa gcccgtacac aatcgtttta acatcttttt cataaatcta
    55081 atgctccttt cattttgggt ataaaaatag acgctcattt ctgaacgtct tacatttaaa
    55141 aagggatact attttaacaa gtacccctaa agtttagtat ttaattgttt gtgttcccac
    55201 actctatatc aactactacc gaaacattga tatagttttt tatctcttta tttctgattt
    55261 tatcgccctc gctcgtaaag acgatatgct tgaaaaagtg cctaaaatta aggatttcta
    55321 catagttcgt ctttgtagca acacacaagt ctccacatgt gtagaatgag ggaacatatc
    55381 tacaggttgt gcctcaacac atttaaatcc gtgaacattt agatagtcta aatcccttgc
    55441 taaagtagaa ggattacaag aaacatagac aattctttcc ggattcattg aaacaatagt
    55501 ctctaatacc ttttcgtcac atccctttct aggtgggtct acaactacta cattggcagt
    55561 cttaccctct ctatacatct taggtacaat ttcctctgcc tttccaacat aaaattcagc
    55621 attatgtata ttgtttaatt tagcatttat tttagcatct tttatggcat catctataat
    55681 ttctattcca taaacctttt tagccttttg tgccaaaaat aaagatattg tacctatccc
    55741 acagtaaata tcaaaaacag tatcagtatc atttaaattt gcatattcaa gagccttatc
    55801 atacaatact ttagtttgga cagggtttac ttggaaaaat gataatggtg aaatctcaaa
    55861 cactaagtca cctatataat cgtttatttt tccatctcca tatataacta tattttctct
    55921 tcctagaata gcattcgtat ctttttgatt tacatttaca accaaagtct taaatccagg
    55981 tatattttcc ttaagcactg aagctaattc tttaagatat ggaagtttgt ttccatttgc
    56041 tactaataca accattactt ctttagttgt aaaacctatt tttgttacta aatgcctaat
    56101 caaccccgtg tgagtctttt catcatatat acttacctta taggccctta tatatgtttt
    56161 tattatttta acaattttat catttatctc atgttgtatt acgcattttt ctgtcgatat
    56221 tacatcatga gtcttctttt tatagaagcc aataattgga ataccattat tcttttgtat
    56281 tggaaattgt gctttatttc tatatctata tggattatcc attcctatag tgtcatgaac
    56341 ttctacatcc tctaacttac ctattctcga aagtacttgt ttaacttcat ttgttttcat
    56401 ttcaagttgt ttattataat ctaagtcttg tatctgacac cctccacaat ccctaagatt
    56461 ctcactacac actcttttca ccctgaatgg agacttttct attatctcaa caatatctcc
    56521 tacagcataa tttttcttta ctttattaat cctaacttta actttatcct gtattaatcc
    56581 gccctctaca aaaactgtaa taccattgta ctttcctact cctactccgc cttgaccaat
    56641 gtctactata tctacaatat acatctcgtt tttcgaaagc ataaatcacc ctccaatcaa
    56701 tctattatat ttaaatcttt acaaactttt ttctttaaat ttatattaaa atattgctat
    56761 taattaaact atatttttta ctaagctaca atattttatt ttaccaaaaa aaaaggagct
    56821 tataaagccc cttagtaaat aaatttatta tttttcttat aaaactctag ttgaacctct
    56881 atataccaca cctcttgatg catccacagt tacaacttct ccatctttta ctagatttgt
    56941 tatatcagtt gcagacacaa taactggtat atctaaattt atacctacaa tagctgtatg
    57001 tgatgtcatt ccaccattct cagttactac agctcctgct ttttctatat aattgttcat
    57061 ttcagcatca gtagcagtag aaacaagtat gtcgccttca ttaaagtcta cacaagcatt
    57121 tccattcttt attatacgaa ctttaccttc taccgtttga gtacctacac ctataccttg
    57181 cacgatttct tcgcttatta catgaacctt tattaaatta gtagttccac ttactcctac
    57241 tggtactcca gcagttataa ctactaattc cccattatct atataatcag cttgtctagc
    57301 tgcctcaatt gccttttcta taacttcatc tgtatttcct gcaacagaag acttaactgg
    57361 atatactccc catgtaagag ctaatttatt catagtcttt tcattatttg tagtagctat
    57421 tattggagat tttggtctaa atttagatac cattctagca gtatatcctg aagaagtaga
    57481 tgttattata gctgatgcat ttaaatctac tgctgttgta caagttgcat ggcttattgc
    57541 atctgttaca gtaacattgt tagtaccatt ttcctttaat aatctatcat aatctaaagt
    57601 ctcttctgtt ctttttgcta ttgtagccat catttttact gcctctacag gatattttcc
    57661 agcagcagtt tctccagata acattatcgc atctgttcca tcatatatag cattggcaac
    57721 gtctgtaact tctgctcttg ttggtcttgg atttcttatc atagaatcaa gcatttgagt
    57781 ggcagtaaca actggctttg ctaattcatt acattttttt atcatcattt tttgaactat
    57841 tggcatctct tcagttggta tctcaacacc taagtcacct ctagccacca ttataccatc
    57901 agaaactttt aatatttcat ctaggttctc tacaccttct tggttttcta ttttagatat
    57961 tatttgtata tcagttgcat tattgttctc taatacttct cttattgcta gtacatcaga
    58021 tgcctttctt acaaaagaag cagctatata atctattcct tgacttattc caaactctat
    58081 atcacttata tctttaggag ttattgctgg taaatttatc ttaacaccag gtacatttac
    58141 tcctttatga tttttaacta ttcctgaatt ttcaacaaca caaactatat cttctccatt
    58201 tatttctttt actcttaagc caactaaacc atcatctatt agtatagaat ctcctgattt
    58261 aacgtcttca gcaagacctt tatagcttac tgtacacatt tctttagtcc ccataatatc
    58321 tttcatagtt atagtgaact tttgaccttc ttctaaaaat acctctgggt cttcaaagtt
    58381 acctgttcta atttctggac ctttagtatc taataatata gcaactggct tatttaattt
    58441 ttctctaact ttctttgcca tatcaattct ttctttatgt tcttcatgtg aaccgtgaga
    58501 aaagttaaat ctacacacat ttagaccatt ttgcataagt tgagttaaaa cttcctcact
    58561 ttgtgatgct ggacctagtg tacaaacaat ttttgtcttc tttacattat ttaacatatt
    58621 tgctcctcct gagatattat tccacagatt tatttttaat actaaaactt atatagataa
    58681 tatttttgcc atctcataag cttctttatc aaatactttc ttttgagcta aagcttcact
    58741 tatttctaaa tcaattattt tgttttcttt tattcctaca actctagctg attttccatc
    58801 taataataat tctactgctc ttactccaag tctacttgct agtattctat cagatacagt
    58861 tgggcttcct cctctttgga catgccccaa tacagtgact cttaaatctg ctcctgactc
    58921 ttttttaagt tccttttcta aatcactagc acttccgact ccttctgcta aaacgattat
    58981 actatgtctc ttacctcttt tttgagtagt ctttaatctt agagcaactt catccactgt
    59041 aatttctact tctggtacta ttatagtttc cgctcctcct gcaagacctg catataaggc
    59101 taaatctcca cagtgtcttc ccataacttc tactatgtta actctttcat gagatgatga
    59161 tgtatctctt atcttaccta tagcatctat aatagtattc attgcagtat caaatcctat
    59221 agtataatct gtatatgcta aatcgttatc tatagttcct ggtatgccta tagctgggaa
    59281 tcctaagtca ctaagttttt gagctcctgc aaatgaacca tctccaccaa ttacaactaa
    59341 gcaatcaatt ttatactttt taagaatttt tactgctttt aatctccctt cttctgtttt
    59401 aaattcttca catctggaag attttaatat agttcctccc ctgtgtataa tatctccaac
    59461 agaagataag ttcatttcag ttaaatcttc ttctagtaat cccttatatc ctctattgat
    59521 tccatatact ttgcatccat aatatattgc tgatcttaca accgctctaa tcgcagcatt
    59581 cattcctgga gcatctcctc cgcttgttag taatcctata gttttcattt tttacctcct
    59641 tggattttat gtcaattttt tattataatc attgttattg tattctaatg gacttttttt
    59701 gtccctatat cactttttaa tcccatcaag actaaaaaaa ttaaatgtcc ttaaatgtcc
    59761 attttgtgat attatatcac aaaacggaca atatttttca tattattata atatatttag
    59821 atttttatat aaaaaatcaa gcttttactt ttatgttatc tttaggaagt aactcttcta
    59881 agttatttat aaattcatca catatacaag tcaatgcccc ggtcatttta tatgctttac
    59941 gtttaccatt tatattttca tccatagggt ataagtatat gctgtcatta cctggatatt
    60001 gtttggctat ttctacaata ttattagttg tatcggtatc agacatacta tctattttca
    60061 aatacaattt tttaccactt tctggcttaa tattttctac atttctagtt cttcctgaat
    60121 tgtagtttct atctgcaaag tcagtatcat catttatatc ttttatctct ctagctataa
    60181 gttttgcatt ttcgccttct tttatactta atgttccttt tacatatatt atattatctt
    60241 cattcaaaat gacattatac ttttgtaata attgaggaaa aactattatt tctatagcac
    60301 catacaaatc ttctaattca ataaatgcca taatttcatt tcgttttgtt gttttaatag
    60361 ttttagttac aatcattcca cccattataa catctgtttc attcatttgt aggtatgact
    60421 cttcttcctc ttttaatgaa tttaattttc cattatccat agatgttttt tcttgtagtt
    60481 ctttttcaaa ttgtgataaa ggatgtccac ttacatacat tcccaagact tctttttcaa
    60541 ggttaagtct ctctctttct tcgtactctt gccttttagg taaactgtat atttgttgaa
    60601 aatcactatc ttcctcacta tctccaaaag catcaaataa agaaatttga ccttgaacat
    60661 ttttctttct atccatagat acactttcta ataaactttc ataaccagcc atcaaactag
    60721 ctctattttc acttatatta tcaaaagctc cacattttat tagactttct attactctct
    60781 tatttgtatc tttttggtct aatcttttga ctaagtctac taaatctaca aataatccat
    60841 tagattctct ttcagcgatt atattgttta ttatatttac accaacattt tttacagctg
    60901 ctagaccaaa tcttatattg tcgccttcaa ctgaaaactt tgaaaaactt ttgtttatat
    60961 ctggaggtaa tacatctatt tctagtactt tacattctct tatatactca actaccttat
    61021 cagtatttcc cattacactg gttatcaatg ctgccataaa ttcaactgga taatatgttt
    61081 ttagataagc tgtctcatat gctaaaaccc catatgcagc tgcatgtgat ttattaaatg
    61141 catatctagc gaagtctatc atgtcatcaa atattttatt agctatttcc tcttctacac
    61201 catttctgat acaccctgca atttctatat taccatcttc atcaaattta ccatgaataa
    61261 aatattgtct ttcttcttcc atcacgtcca tctttttctt actcatggct cttctaacta
    61321 ggtcacttcg tccataacta taaccaccta aatctctaac gacctgcata acctgctctt
    61381 gatataccaa acaaccatat gtaacttcca ttataggctt taatttttca tgtagatatg
    61441 ttacatcttt tgggttgctt ttatttttta tatatgcagg tatagaatcc attggacctg
    61501 gtctaaatag agatattccc gctacaatat cttcaaaatt gtctggtttt agctgtttca
    61561 taaaacttct catgccagca ctctccaatt gaaacacccc tagtgtattt cctgatgaaa
    61621 gcatttcata tactttcggg tcgtcataat ccatctttga aaagtctatt ttttctgtgt
    61681 agttttttat aactctattt ttttctatta aatcaagagt atctcttata actgtaagag
    61741 ttcttagccc aagaaaatcc atctttaaaa gcccaagttc ttcaagtgtt gtcatagtaa
    61801 actgtgttgt gattgcatct tgatgtttgt acaaaggtac atattcatct acgggatttt
    61861 ttgatataac aactcctgct gcatgagtag aagcatgtct tagcattcct tctatctgtt
    61921 ttgatatatt tattatttct tttgtttccg ggtcttggtc atacaattct ttaagattag
    61981 gatttgtatc tagggcctta tctattgtca ttcctaaagc aaatggtatt tccttggcta
    62041 ccttgtcaac tttattgtag cctatatcca atacccttcc aacatctctt attgcagctt
    62101 ttgcacccat tgtaccaaaa gttattatct gggctacatg gtcatcacca tattttctct
    62161 ttacataatc tataacttct tctcttctct cataacaaaa atctatatct atatctggca
    62221 tggatatacg ttctggattt aaaaaacgct caaataatag agagtacttt attgggtcta
    62281 tgtcagttat tttaagtgta tatgctacta tagaaccagc cgcactacct ctacctggtc
    62341 ctactataat attattttct ttagaaaaat ttataaaatc ccaaacaatt aaaaaatact
    62401 ctacataacc cattttttca attacatcta attcataatt taatctctcc agtatctcat
    62461 tgctaggatt atcatatctc tcttttaatc cattaaaaca caactccctt aaataagttt
    62521 ctggtgtata tccttctgga acatcatact taggaagatg tatagtatta aaatcaaact
    62581 ccacattaca catatcagct attttcacag tattgtcaat agcctctaat gcatatggaa
    62641 ataattcttc catttcttct cttgatttta aataaaactc atcactacca aatctcattc
    62701 tatttgggtc atttaatgtt tttcccattt gaatacacat aagaacatca tgtattttcg
    62761 aatcttcttt atttacatag tgaacatcat tagtagcaac tagtggtata ccagtctctt
    62821 ttgataattt tactaaacca gagtttactt ctttttgctc aggtaaattg tgatcttgaa
    62881 tctccaaaaa gaaattttct tctccaaata tgtctctgta gtcaagagca aattgttttg
    62941 ctttttcata atttctattc attaaagcac gtgctacatc tcctgcaagg catgcagaaa
    63001 gtgctataat ccctttgtta tgttttttaa gttcttccat gtcaactcta ggcttatagt
    63061 aaaaaccttc tacgtatgaa gttgaaacta tttttattaa atttttatat ccttccatat
    63121 tcttagctaa cagaactaaa tgtccttgat atttgtcata gtttgggtct ttatctctca
    63181 atccacgtgc agctgtatag acttcgcacc ctataattgg ctttattcct tcttttatag
    63241 ctgttttgta gaaatctata acaccaaaca tacaaccatg gtcagttata gctattgagt
    63301 tcatgtttag ttccttagcc ctttttataa gttttttcac cctcgaaaac ccatcaagta
    63361 aactatactc agtatgtaca tgaagatgag caaaatcttt gttcattaaa tcacctcctg
    63421 ctttataatt ttaattttac cacaaatata tgtactaaaa caaaaacaga gacttttatc
    63481 tcaaaatagg aattaacttc aacgtatacc attatatgtt ataaattaat gcttattctg
    63541 agattgtccc ctcaatttat tgctatgatt tttttttcaa atctgttgca atttcttcta
    63601 tttttctaag tctatgattg actccagatt ttcctattgg tggttctaac atctctccaa
    63661 gttctttgag agtcatatcc tcatactcga ttctaatctt agctatctct tgtaaatttt
    63721 ctggcaaact gctaattcct atagtctctt gtataagctt aatattttct acttgtctta
    63781 ctgctgcatt tactgtttta gaaaggttag ctgtctcaca atttactatc ctgtttacat
    63841 tatttctcat ttcttttact attttagtat tttgaagact taaaagagcg tggtgtcctc
    63901 ctataatact caacaaatct gatatttgtt cactctcttt gatataaaca acgtgattat
    63961 tttttctagc cacaatttta ctattaaaac ctaaagagtt tataagttct tttagagaat
    64021 ctgcaaactc ctcattatta gttacaaatt ccatatgata gttcttttct gggtcgctta
    64081 tagaacctcc accaagaaaa gctcctctta tgaaagctct cttacatcct tcatgcttaa
    64141 ttaagttctc tgaaattttg tttatagtaa aaaatccttc tttatcttca agtataccta
    64201 acttttttaa taacatttct gaacccattt cgcttgttac cattaataca taactgttat
    64261 ttctctttag catttgattc ttatttactg agattgtagt attgatatta aaattctttt
    64321 ttaataattt aaatacttta cgggctattg aatttaactc tgttgttatc tttagattta
    64381 gcttcttata tccaacaatt tgtatacttc cccctgattt aactagagct gacaattcag
    64441 ctatattaca acattcattc ccagatataa ctcttgctag ttcatttttg gtttcagttg
    64501 aaaaagacat atctatcacc tattaactaa tttttcttta accttttttt tatttcttgt
    64561 aaggcaacct ttattatctc cttttcatca tcatacctag ctaagtctac tactctatct
    64621 aggtggataa atttagcatc aataaagcct tcctcttttt gtggaatagt atccatattc
    64681 tttgcttgca tcaaatacca aaaaactgtt ttatgaactg ctctattttc atcccagttt
    64741 tctttgaatg tatagtgtat ttcacctaaa tatttcaata tatctgcttt tactccagtt
    64801 tcttcactta cttcacgtaa tgctgcctct tggttatttt ccccttcttc tacttttccc
    64861 ttgggtaaca cccaatctcc attgaacttc cttagtaaaa gaatcgtatt gccaaacagg
    64921 actacaccac cagcactaac ctcttctctc atcgatgttc actccttaaa actaacataa
    64981 tgataaggat ttctacacaa ttttttaaaa tcctttttta ctaacttttg ttgtaattat
    65041 gatttagggc taactcaata actatgttag atatatactt tgcatcatgt cttatataat
    65101 tattttttat ttcaattaaa ttcttctcta cagtttttat acccatctct tttaatttat
    65161 ctttttgctt tttatctaac aagacttgct ctgctccatc ttgtttatat aagtcaagca
    65221 tttcttctgg caatatctca ttatttgcga ttacataatc tattaaattc tcatcagtgt
    65281 gttctactat agcattaaca tgctctaata cattgtaccc ttctgtctca cctggttgag
    65341 tcattatatt tgctatatat acttttggag ctacagacga tttaatggcg tctacaatcc
    65401 cctctacaag taaatttggt attatgcttg tatataagct tccaggaccc ataattataa
    65461 tgtcagcatc ataaatagat gcaataacat cctttaatgg ttttacatct tttggattta
    65521 aaaaaatttt atcaatagag cttttttgaa tttttgactc ttcaggtata ctggattctc
    65581 catttattat attgccattt tttaatttgg caactaaatt tacatcttcc aaagttactg
    65641 gtaaaactct tcctgttata gcaaaaattt cacttaattt atatacagct ttttcaaagt
    65701 ttccatacaa cccattcata gcagccaaaa acaaatttcc aaaactctga ccttttaaaa
    65761 gcccttctgt aaacctatat tgcataactt cattcattgt tggctctata tttgcaagtg
    65821 ctaataaaca gtttcttatg tctcccggtg ggagcattcc taagtcttct ctcaatacac
    65881 ctgaacctcc accatcatca gcaacagtta caattgcagt tatattttgc gtagtgtgtt
    65941 taagacctct taaaaatacc gattgaccag tacctccacc tatcacaacc acatttggtt
    66001 ctctattttc aacaatagac ctttgaaata gttttacttt ttcatttttt gcttttttat
    66061 atataaataa cgatacaatt aatgcaatcc aacctaaaat tcctacaata ataagtaatt
    66121 tagccatgtt tgcctccatt atcgcaacat agagtctcta tgtttcttta caactctata
    66181 tcccttatta gataaatcct ccgctataag atttgttata gtaactgacc tatgtcttcc
    66241 acctgtgcat ccaacaccaa ttactagatg ttgttttcct tcctctatat attgaggtac
    66301 taaaaaatga atcatatcta aaagtttcac ataaaattct tcactgattt tagaattcat
    66361 aacataatct ctaacttctt tatcatcacc agttttagct cttaactctt caacataata
    66421 gggatttgga agaaatctta catcaaatac taaatctgca tcagcaagta taccatgttt
    66481 aaatccaaaa gatactacag aaatagttag gtttggatta tcttcacctg aagaatatat
    66541 tttctttatt tcttccttaa ggtctttagg tttcatgtta gttgtatcaa ttatacatgt
    66601 agataactcc tttaaaggtt ccattatctt tctttccatt tttatacctt ctggaatctg
    66661 catatctttt gccaatggat gatttcttct tgtcatttta tatctcttta acaatacatc
    66721 atcattacaa tccaaatata ccatttcata ttcataattt tctttttcta aataatttaa
    66781 gctttcatga agcgcttcaa aaaactttct ccctcttata tctatcccta gagcaacttt
    66841 atcaatactt gaatttggtt gataacataa ttctgcaaat ttagttatca aagcaggagg
    66901 tagattatct acacaataaa atcccatatc ctctaaagct ctcattgtct cactttttcc
    66961 agatcctgaa agacctgtaa ctataacaaa tttcattgct aactcctccc ttttatctta
    67021 tgtttattat attataatgt tttctactaa cgatatatcc agattagagt cagatatagt
    67081 cttcatagct agttcaaaat caccaactct ttctggacaa tgtgcatctg agcctattat
    67141 aaatttattt tctagccctt ttattttatt taattgattt acattcaaaa aagaatggct
    67201 actatttatc tcaagtctag tatttgtctt ttttgctgtc tttgctactt cttctatata
    67261 cacatcgcct ttatctcctg gatgagtaat tataaaaata tcattattac ttatcgcatt
    67321 tattacagct tttgtattat attcttttgc tttttcagtt ttaaaaatat agttattaca
    67381 atgatttaat aagcttctta atgatgttgg agtagaacca aagtgatatc cagccataat
    67441 ataatcaaaa tctctaataa atttatcatc catatctata tttcctttat catctaatat
    67501 gttacactcc atacctaaaa gtatatttat atcagtgtac ttatcattta aataatctac
    67561 ttcttctctc attttgagaa tatctttttt ctttacacca tatgcaatat gtttataact
    67621 atggtcagat attcctattg tttttattcc tttagaaatg gctaccttta cattatcctc
    67681 aatagtccct ttgccatgac tatataatgt atgtgtatga taatcagcca gtattttcat
    67741 ttgttaatcc tctccaagta ttttgacttc ttcttccaac ataaccccaa acttggcata
    67801 tacagcactt tttataacat atattaaatc caaaatatct ttagcacttg cttctccttg
    67861 attgactaca aatccacaat gcttctcaga tacttgtgct cctcttaaag taagacctct
    67921 caatccagtt tcttctatta atttacctgc aaaatatcct gttggtctct taaatgtact
    67981 tcctgcacta gcaaaattta atggctgttt agttattctt ctcgtcgcta aatcatccat
    68041 taaacttttt atttcatcat aatttccttc ttgtaattcc aattctgctg ataaagctat
    68101 atatccgttt ttcgatatta tactttttct atacccaaat tccatctgtt catttgataa
    68161 ttcaaatata ttgccttcca tatccatcag tcttactgac ttcactatgt ccttcatttc
    68221 accaccatat gctccagcat tcatggctaa tgctcctccc aatgtgccag gtattcctga
    68281 tgcaaactca aaccctttta attcttgctt ttggagagct ttccctaaaa ctgataataa
    68341 agctcctgac tgagcagtca tcctagtatc atttatctca taagaattga aattatcagc
    68401 tagttctatt accactcctc ttattccacc atctttaatt aaaatgtttg aaccatttcc
    68461 aattattaaa tacggaatag attcttcttt taccaactta agtacatttt ttaattgttc
    68521 ttcagttcta ggtctaacta atatatctgc tggtcctcct accctaaatg aaatatgttt
    68581 tttcataggt tcatcgactt ttatatcctc tttacttaat atatttaata aatttatata
    68641 tatatcttga ttattcattg tatttcctcc caaattatac tgaactttag ctgtcattat
    68701 tatttaaatt acattagtat atagtattaa ctatattcaa taatatatga ttttgcttat
    68761 catatggatt ttatatttta agcttcttaa ttacaaacta tattcaataa atatataata
    68821 aagtaaaaaa aaaaaaataa caacatataa aagaatataa ataatttaaa aaaagcatca
    68881 atatcactga tatcaatgct ttaaaaggca atttaattcg tattatttat ctaactattt
    68941 ttgacatagc aaagataata aatccctcca actactatgg aacctccaac gatatttcca
    69001 agtgttacaa ataccaaatt agttataaat tgaaccatag ttatatttgc tcctaatgcc
    69061 attcccattg gtataaaaaa catgtttgca acactatgtt ggaatccaca taatacaaat
    69121 agcattatta ctgtccaaca tgaagccaat tttgacatta tatcttgaga tgctgtagcc
    69181 atccatacag ctaaaacgac tagaatatta caaagtattg cttttaaaaa cattgatgct
    69241 atagttgaat taaccttagc ttctgccaca gctatagctt ttgtagctgc atctcctgtc
    69301 aatgtagaag aataaaacac aagtgcaact agcaaaattg aacctactaa atttgcaaaa
    69361 taaaccagtc cccagtttct taacattttt cctaatgtta tttttttgtc cataagtgca
    69421 atagccatta aggtgtttcc agtgaataat tctgctcctg ccactactac aagcataaga
    69481 cctactggaa agaccatagc acttaaaaag ctagctaccc ctgggtctat cttgccaaaa
    69541 gtttgactaa ctatcatact ccccaatgcc ccaaaaccta taaatgctcc tccaaataaa
    69601 cctaatagaa ttgcattttg tgtcgaaata tttgcttttt taatgcctgc acttatagtt
    69661 gcttgtgcag tttccccagg tgtcaagaat cctttactca taaatccttc ccccttaaaa
    69721 attatacttt cccttgtctt tttatggcaa taattatata ataacaatat gatatataat
    69781 aaattttatt aacataaaaa atatactata tacttatata tacttgtctc atataattat
    69841 actatttatt atataacaac tttgttaatt tcgaaataat ttatattgaa tttgaaaaat
    69901 ttaccttttg attatttatt ttacttttta attattgagg tattatatat aatagatata
    69961 tgtttgtgtt attatatata attacttatt ttaataaata tttgaaggag ggaattttat
    70021 gtttgatata tcatttttcg aaatgtcaat atatgaaata gcagttacta caatatatat
    70081 aattaatttc atggttatac taaaccttat ctttagagaa aaaagaaaca taaatacaac
    70141 tttaacatgg cttcttatat tagtattgat tcctgctctt ggattcatac tttatatagc
    70201 ttttggcaga aatatttcaa aaaacaatat gtttagattg aaagaaaagg atgataagat
    70261 aatcaaaagt aatatactag atactcaggt taaactccaa tctacatctg aaattgattc
    70321 agatatacat caacataagg atatgattta tgcattggct aattcaaata atgctcatta
    70381 taccaataat aacgatgtgt ggatttatgc tgaatctagt caatttttta acagtcttct
    70441 tgaagaactt aaaaaagcta aaaaatatat aaacatacaa ttttacattt tcaaagatga
    70501 caaaatcgga actgaaataa tagacattct tatagacaaa gctaaagaag gtgttgaagt
    70561 aagactttta tttgatgcag ttggtggtag aacactaaaa aatagtacac tttccagact
    70621 taaagaaagt ggtgttaaag ttggaagctt cttcccatca ttcttgaaaa ttgtaaactt
    70681 taacttaaac tatagaaatc accgaaaaat tgttgtcatt gatggtaagg taggatttgt
    70741 aggtggatat aatgttggtg atgaatattt aggtagaaat ccaaaatttg gtctatggag
    70801 agatacacat actaagctta ctggtgattg cgtaattgac ctaaatatga gattcatcct
    70861 tgattggaga tatacaacta aagaagattt agatttagaa aagtacttta tagaagaaga
    70921 tacaaattct aatcatcctt ctgaaaatat tgggattcag atagtatcaa gtggtccaga
    70981 tatatcagaa cttgatgaga ttaaatatgg ttaccttaaa atgattcaga aagctagaaa
    71041 gtatatatac atacagagtc cttatttaat acttgactca acttttatag atactttaaa
    71101 aatagcttgt ttatctggtg ttgatgtgag agtgatgatt ccttctaaac ctgaccatcc
    71161 ctttgtatac tgggcatcat attcatatgc tggagaactt ttaaagtttg gagctaaaat
    71221 ctatacttat ggtcaaaatg catttttaca tgctaagaca atagttatag atgactctat
    71281 atgctctata ggaacagcta atatggatat cagaagtttt gagcttaatt tcgaagttaa
    71341 tgcatttatg tactcttcaa aaaaagcatt ggaacaaaga attatatttg aaaatgatat
    71401 tttaaattct aaggaaataa cattagatat atataattct aggtctactt atataaaaat
    71461 caaggaatca atttcaagac tattatcagc agttttataa atcttttatt ttataaaaat
    71521 tttacatttt ggtttatttt ttaaagtttt ttatatgtat tatttttttt ttattgaatt
    71581 tagctcattt tttttatttt ttttaactat tctgaattat tttatattat aatagactag
    71641 tatagttttt atttttaata ggtaactttt ttgcattgtt tttataaata aagaaattgt
    71701 tgtatattat tctttcttta ttgttttggt tttgttcacc cacataatgt tggggtcaac
    71761 tcatggttaa ttattttatg aaaaataata actttaaaaa gaaaaggggg atttgttatg
    71821 tgcgatttta tttcacacaa caagcaatta tttgacgaac tagatatatt tattgactct
    71881 cttgcaacaa aggaaggtgc gttaatacaa gttctacatg aagcccaagg tatatttgga
    71941 tatcttccaa aagaagttca acttcatgtt gctagaaaac ttggagtagc tcctgcaaag
    72001 gtatatgggg ttgttacatt ctattcttat tttactacag aacctgtagg taagtataaa
    72061 atcagtgtct gtctaggaac tgtatgtttt gtaaaaggtg ctgacaaaat cttaagtgct
    72121 tttgaaaaac agcttggcat aaaagttgga gaaactacta gtgattttaa attttcttta
    72181 gaaggtttaa gatgtttggg ggcatgtggt ttagctccag ttgttactgt aaatggtaaa
    72241 gtttatggta aagtgaaacc tgaccaagta agcgaaatat tagatactta tagagaatta
    72301 gaattgaatt gttaagaggg gtgaataaat aatgcgtaaa gtaaattcat ttgatgaatt
    72361 aaaagtttta gctgatgagc tgaaacctaa tcttagctta agaaaaaatt attctaagga
    72421 aaatgtatta agaagagaat tacttgtttg ttgtgatact ggatgtacat cttcaaacag
    72481 tctagaaata gttagtgaat tagaaaatga aataaagaaa tcaggaatcc aagataaagt
    72541 tagtgttcgt ttgacaggtt gttttggttt ctgtgctcaa ggtcctattg taaaagttta
    72601 tcctgataac gttttctacg taaaagttga gccatctgat gctgaaaaga tagttcaaag
    72661 ccacttaatt agaaatacag ttgtcgagga attattatat gaggaagaat ctttaagtaa
    72721 aaaagttgaa gaacaagaag aaatgtcttt ctacaaaaaa cagttacgta tagctttaaa
    72781 aaattgtggt cttatagacc cagatagtat agaagaatat attgccaatg atggttattt
    72841 agctctaggc aaatgtttaa cttctcttac tcctcaagaa gttattgcag aggtaaaaac
    72901 ttctggcctt cgtggtagag gtggtgcagg tttccctact ggctctaaat gggaagctgc
    72961 ttcaaaaaat ccagctggtc caacagataa aaagtttgtt gtatgtaatg ctgatgaagg
    73021 tgacccaggt gcatttatgg atagatctgt acttgaaggt gaccctcata gtgtactaga
    73081 agctatggca atatgtggat acgctatagg ttctgataca ggatacatat acataagagc
    73141 agaatatcca aaatctatag aaagattaaa agtagccata gctcaagctg aaaaatacgg
    73201 tttacttggc gaaaatatct taggcactgg atttaacttt aaattagaat taaaatatgg
    73261 tgcaggtgct ttcgtttgtg gtgaaggtac agcgctaatg cattcaattg aaggtagacg
    73321 tggtgaacct agaatgaaaa cttatagttc ttctaagagt ggtctatgga aatctcctac
    73381 ttgcttaaat aacgttgaga cttttgctaa catacctgca attatactta agggtggaga
    73441 ttggtttgcc aacttaggta ctgaagattc tagtggtact aaagtctttg ctcttggtgg
    73501 aaaggtagaa aatgtaggac ttgttgaagt tccaatgggt actactttaa gagatattgt
    73561 atttgaaatt ggtgggggaa tccctgaagg taagaaattt aaggctgttc aaacaggtgg
    73621 accatctggt ggatgtatac caactgaaca tttagacact cctatagact ttggttcatt
    73681 aagttctatc ggttcaatga tgggctctgg tggtatgctt gttcttgatg aatctgactg
    73741 tatggtagat atagccaagt tcttccttga gtttactgtt gaagaatctt gtggtaaatg
    73801 tacaccttgt cgtataggta ctacaagatt attagaaata ttaactaaaa ttacagaagg
    73861 taatggtact ctacaagatt tagatgacct agaaaacctt gctgaaacta tacaaactgc
    73921 ttcattatgt ggtcttggta aagctgctcc aaaccctgtt ttaagtactc ttcaatattt
    73981 caaagatgag tatatagctc atgttgtaga taagaaatgt cctgctggta aatgtcagaa
    74041 cttactttct tactttataa cagatgactg taagggttgt acaaaatgtt ctagagtgtg
    74101 tcctgctggt tgtataacag gttctgttaa ggaacaacat acaatagata cttctaagtg
    74161 tctaaaatgt ggtgcttgta tagataattg tacatttaac gctataatca agaaataact
    74221 tatttggagg aggaataaca atgagtttag ttaatttaac tattaatggc aaacacgttt
    74281 cagcacctag tggaacttct atcttagatg ctgcgaaatt aataaatata aaaatacaca
    74341 acctttgtca tttacatatg aatgaaatag ataagcttga cacttgtgct tcttgtcgtg
    74401 tttgtatggt tgagacagag agaggattag ttccagcttg tggaacagtt ataaaggaag
    74461 gtatgaaagt tcagactaat agtgctaaag cattaaatgc tcgtagaact attgttgaat
    74521 tattgttatc agaccatcca caagattgtt ttgtctgtga aaaaaatgga gattgtgaat
    74581 tacagactat agctgctgat ttaggagtaa gaaaaataag atatcaaggt tctaaatctt
    74641 ttgcaggtaa agatacttct actaaatctt tagttaaaga ccattctaag tgtatattat
    74701 gtagaagatg cgagactgta tgtaatgaca tacaaacagt aggagcatta tctggagtta
    74761 atcgtggatt taatacttta gttagtacat tctttaactc tgatatggtt gaaacagagt
    74821 gtacattctg tggtcaatgt atatctgtct gtccaactgg agcattaaca gaagtagata
    74881 atgtacctaa actttgggat gttttaaata aaaaagaaaa gactatagta gtgcaagttg
    74941 ctcctgctgt aagagttgct attggtgaag agtttggatt agaaccaggg tcaatctcta
    75001 caggaaaaat ggtagctgct ttaaaagcac ttggatttga acatgtattt gatacaaact
    75061 ttggtgcaga ttttactata atggaagaag ctactgaatt tatagaaaga atccaaaaag
    75121 gtgaaaatct tcctatatta acaagttgtt gtcctgcatg ggtaaacttc ttagagcaca
    75181 attatccaga caagttaaac ttagcatcta gctgtaagtc tcctcaaggt atgtttggtt
    75241 ctatagctaa aaactactat gcacctaaaa tattaggaat caatcctgaa gaactttatg
    75301 ttgtatctgt aatgccttgt gtagctaaaa aatatgaagc ttcaagagaa gaattaagtg
    75361 aatctggaat actagatgtt gacctttcta taactactag agagttagct aaaatgataa
    75421 aagaagctgc tatcgattta cctaatcttc aagaccaaga ttttgataat cctctaggaa
    75481 aatctacagg tgctgcatct atatttggag caagtggtgg agttttagaa gctgccctac
    75541 gtacttcata tgagaagata actaataaga ctttagataa tgtaaacttc actaatgtta
    75601 gaggtcttaa aggaattagg gaagcaagta tagatgttga tggaactaca gttaatgtgt
    75661 gtattgtaaa tacacttaaa aacgctagaa agattatgga taaggtcaga agtggagaat
    75721 gtaaatacca tatcatagaa gttatggcat gtcctggtgg atgtgttgga ggtgctggtc
    75781 aaccttatca ccatggtaat actgaaatag ttgatagacg tgcaaatgcg ttatatgaaa
    75841 tagatagaaa taaggctatt cgtaaatctc atgaaaatcc tgatttacaa gctatttata
    75901 aagacttctt tggagaacca aatagtgatg tagctcataa ataccttcat actcattact
    75961 ttgataagag ttgtgtctat ggagagtgtc cacaagaatg tgcttgtgaa gaagctaagt
    76021 agtcataaca aagtataaaa tttattctta aaaaaggatt gtcttaaaat acttttacgt
    76081 aatttaagac aatccttatt aatttcacaa attaaattat atttctaaat agacttattc
    76141 tttaattaac ctatctatta attaaagaat acctgccatt ctcataagtt gttttgcatt
    76201 tgtagtttta catcttccat cttttatctt atagtcaaaa tgaattttat cgtctacata
    76261 gtactctgag aaatgatagt ttacaacttc aacatctcca ctacctgaaa ggtcacatag
    76321 ctcaaagtca tgtgttgtca ccatacttat aacccagggt tttgataatt tctttaccgc
    76381 ctcacttgct ccaataattc tatcagcaga gttagttccc ttaaaaattt catctactag
    76441 aaccaacatt ggcagttctt ttaaactata ttgaatcatt gattttattc taagaatctc
    76501 agcatagaaa gttgatattc cttgacttac atcatcttgt attctcatag aagtaaatat
    76561 agccatacag gtcgcatcaa aactcttagc gcatactgga gcacctgcat atgccagcac
    76621 taagtttaca ccgatacttc ttaaaaatgt tgtctttcca gacatatttg aacctgtgat
    76681 aatacaagtc tgagaattta attgtatcga atttgctatg acactttttt ctgctattaa
    76741 tgggtggtat acctcttcaa ctttcaatct tggagtatct tcatatttta ttactggaaa
    76801 acatgtattt tcctttacat gagaaacaac tgttaaactt attagagctt caaattctcc
    76861 aatagattca aaccaatctc taatttgatt tccatatata cctttccatc gctccaaagc
    76921 ttctgcacaa tgatagttcc acatcaaaac accacaggca attatatata gtatctgatt
    76981 ataccttagg tttacagcat ttcctattga atttaattgt ttaacacctc ttgaaactcc
    77041 acttccctta gaaagtttat cttgtaattc tttcaaatat gcactctcaa acgtctcttt
    77101 ttctaaaacc tcaaacattt tttcatatac ttgtatactt ctactaaatg aaaataatgg
    77161 agcaagagtt tgcataagtc ttggatatcc aaaaccacta aaccctaact ggattataaa
    77221 aagtaaaaaa acaggtaaaa caggtaaaaa tcctaacata caaaatataa aagataaaat
    77281 tgttgcagtt ggtagacccc aagtaaaaat atgcatccat tttggtatat acacattctt
    77341 attttctcca tactctataa acgattctat actactatct gaattatttt tttgctcttt
    77401 accaattata ttactgagtg tttgaatatg aatagaaaaa tcatcttttg ataaaagttc
    77461 ttttatagct ctctgtcttt ctaagattac attctcatct ggattttcat tccacaaata
    77521 tttggctaaa aaatttttcc cataagatgt attagctaca cagatatatt gatacaatga
    77581 tgctcttcca aataagtcta aatcttttgc ttgcgagttt tcatctttta agtattcttt
    77641 accatctatt ttaaattcct tccaaccatc tccaaatctc ttaatatact tatttaatac
    77701 ttcgctctta cttttaaaat atgtttcttc ttcttttact ttactatgaa taactaagag
    77761 tgctataaat attattattg ataaaactcc aacaaaaata cctactatat ttttattgta
    77821 aaatccaata gcaaaccctg ctatcactat caaaaaagat attaatcttg ctgtacttat
    77881 gttatttgac tttttactta agagcaattc tttttcagta ctactttcta aaagtttact
    77941 aaaatattct attcccataa aaaattccaa ctttcttgtt atataatgaa actcaaatat
    78001 aaaattaatc tagtattatt ataatctaac actgtacttt aaattatcct tatataatat
    78061 atcttattaa taaacattat atatatacaa cttgatttta atttataagg tgtaggacat
    78121 aatcctaatc aatatttaat taaatgatgt gtagttcact attattcagt actacttagt
    78181 atcgattaat tttgataatt ttttagtaaa ttcttgagct gcatcgtatc ccattcctct
    78241 ttgtctaaaa ttcattgctg ctacttctaa tatcatagca gtatttcttc caggtttaac
    78301 aggtacaact aatttctcta cattaacacc aagtatttct tcatattctt tatccagtcc
    78361 cagtctatca taatacttag tttcatccca agcttctaaa tgaactacta aatctatcat
    78421 tttagaattt ttgattgccc ctactccata taaacttcta acatctatta ttcctattcc
    78481 tcttatctca agaaaatgtc ttattagttc aggtgactga cccacaagca tactttcatc
    78541 taattttctt atttctacag catcatctgc aactaatcta ttaccccttt gaatcagttc
    78601 taatgctgtt tccgactttc ctatactact ttcacctttt atcagtacac ctataccata
    78661 aatatctact agcaccccgt gaattgttgt gtgtggtgct aacttattat ccaaatagtt
    78721 tgacagtcta tttataagtc tagtggtatt tctatttgtg ctcaaaagta ttcttttata
    78781 ttttttagcc ttttcaatta catctggctt gatttccaag tctctagaaa ttattaacgc
    78841 tggtatttca tatgagaaaa atctatctag tacttcttct cgaatatgac tatccattaa
    78901 tccaaaaaaa ttatattctg tctttccaac tatttgtatt ctttcatatg caaaataatc
    78961 aaaatatcct gcaaactgta atcctggtct atttatatct tgagagtaga cataataatc
    79021 tacatcatca ggcatgtata ctacttttaa cttcaactct tttattatct ctcttattga
    79081 tactttattg ctagtttcca ttaattttct tcctttctaa aaaaattata aacattatct
    79141 gcaatgcttt tattcattcc atctacttct aaaagttctt ccatagtagc tttttttata
    79201 gcatctacat ctttaaagtg atttaataat gatttctttc ttttttcacc tattccttgt
    79261 atatcatcta agatagattt tgtcaaagct ttatttctta gacttctatg atatgttatt
    79321 gcataattat gaacttcttc ttgtatacta gcaataaatc tatataaatt agttgtttta
    79381 tctagttcta tttctttttc tttgcatatc aatcctttag ttctatgctt atcatctttg
    79441 tacattcccc aaagaggtat atctacatca ttgagttcta gtactttttt tactgcacta
    79501 acttgtcctt ttccaccatc caacaatatc aagtcaggta aatttccatg ttttaatctt
    79561 ctatctacaa tttcagccat agaatcataa tcattaggtc caataactgt ctttatttta
    79621 tatctcctat attccttctt atctttttta gcattcgtat acactaccat agaaccaatc
    79681 gaatctacac cttgtatatt tgatatatca taagcctcta ttcttattgg caacttttct
    79741 agattcaata tttgctttaa ttcttccaat gctccaatac ttttttcata ttttctttta
    79801 ttcatatcag aaaatttttc aagatattct acagcatttt tcctaaccat ttcaactaag
    79861 cttttcttct ctcccttttg aggaactctt attgttactt tttgcccctt tttagatgac
    79921 agccattctt ctaatataga actatcttct atctcatctt ctataattaa ttctttaggt
    79981 atatactctt gttcattata aaattgtttt acaaatgaac ttaatataga agctcttgga
    80041 ctatccatta caccttctaa tataaaatgt tccctaccta caatcttacc atttcttata
    80101 aaaaatacct ggacacaagc ttcattatga gctcttgcca ttgcaaccac atcttggttt
    80161 aagtcactta cagtagcatc tattttttgt ttctgtatca tctcttcaag gctctttatc
    80221 ttatccctat atacagcagc ctcttcaaat ctaaagttca tagaactctc attcattttt
    80281 tcctttaaaa gttccatcaa tttttcttct ttaccagata aacacattat aatctcttct
    80341 atcatttttc catactcttc tttagaaaca tttcctgtac aaggtcctaa acatttattt
    80401 atgtgtagat ttaaacaagg tcttgtctta ttctttattg ctttatctat atctatttta
    80461 cagcttctta ttggatacgt actacttata agctccaagg tatcattcac agcagttata
    80521 ttggtatagg gcccaaaata ctttgcctta tctttaagaa ctcttctaac ttttaagatt
    80581 cttggataat cttcattcgt agttactttt atatatggat aagttttatc atctctaagc
    80641 actacattat actttggtct gtatcttttt attaaattac attcaagtat taatgcctct
    80701 aactctgaat ccgttataat atactcaaac ttatatatat tcttaaccat agattttact
    80761 ttagatgtat ggttttttga agattgaaaa tactgcctta ccctattttt tagagatatg
    80821 gcttttccca catatattat atggtcgtat ttgtctttca ttaaatatac tccgggctca
    80881 gaaggtaatt tttttaaatg ctcttgtata tcaaacatct cgccctccaa aacattaaat
    80941 tttatactaa aattataccc taaaatatga attttaaaaa gttatacaat ttccagtgct
    81001 ttagacaagt taaagcccat attcccttct ataattgatt tgaaaacaat tttaaccatg
    81061 aagagtaata tggactttac tttgtattac tatttacaac ttatatttga ctaatttact
    81121 tataacaaaa cagtttatcg tatatctgct ctaaaatttt tcacaattta tataacttaa
    81181 gaaaaatatt tctttaagaa ttgacctgta taagaaccat caactttaga tacttctttt
    81241 ggtgttccag ttgcaactat agttccaccc ttatctccgc cttctggtcc taagtctatt
    81301 acataatcac aagtctttat aacatctaaa ttatgttcta tgactataat tgtatttcct
    81361 gtatctgcta gtctttgtaa tacttggatt agcttgtcta catctgccat atgaagtcca
    81421 gtagtaggtt catctaatat atacaaagtc tttcctgttg gtcttttact aagttcagca
    81481 gctaatttta tacgttgtgc ttctcctcct gataattgag ttgaaggctg tcctaattta
    81541 atatatgaaa gtcctacatc cattaaagtt tctaattttc tttttatatt tggtatattt
    81601 tcgaaaaact caacagcttc ttccacattc atatctaata tatcagatat agttttatct
    81661 ttgtatttta cttgcaatgt ttctctattg tatctttcac ctttacaaat ttcacaagga
    81721 acatatacat ctggtaagaa atgcatttct atttttatta ttccatcacc cttacaagct
    81781 tcacatctac caccttttac attaaaacta aatctacctt ttttataacc tcttgcttta
    81841 gcttcattag tagatgcaaa aatatctctt ataaaatcaa atacacctgt ataagtagca
    81901 ggatttgaac gaggtgttct tccaatagga ctttggtcta tatttataac tttatctata
    81961 ctttcaattc cttttatctc cttatgctta ccagctcttt gcttaagtct attcacttta
    82021 cttgcaacac ctttgtaaag tatatcgttt attaatgtac tttttccaga acctgaaaca
    82081 cctgttatgc atgtaaactt acctaatggg aactttacat caatattttt cagattattt
    82141 tctgatgctt taactatttc tataaaactt ccatttcctt ctctagtagt ctcaggaagc
    82201 aatatttgtt ttttaccact taaatattga cccgttattg agttctcatt ttcaattatt
    82261 tcatctaaag taccctgtgc aactatttca ccaccatgta tacctgcacc tggaccaatg
    82321 tctataacat aatcagcttc tctcattgtg tcttcatcat gctctacaac gattaaggta
    82381 tttcctaaat ctgttagatg tctaagagta gttataagcc tatcattatc tctttggtga
    82441 agccctatac ttggctcatc aagtacatat aaaactccaa ccaatgcaga acctatttga
    82501 gtagctaatc ttatacgttg cgcttctcct cctgataaag ttccagcttt tcttgataaa
    82561 tttaaataat caagacctac atctcttaag aacatagctc ttccttttat ctcttttata
    82621 atctcatgag ctataaatct ctgtttttct gtaagattta tattttccat aaagtcaatc
    82681 aattcattta cagataagtt tgtaacttcc attatatttt ttccatcaac taaaacagat
    82741 aaaacttcct ttttaagtct catcccttta cactttggac atggtctctc tgccatgtat
    82801 tcttcaatct tatctctcat gtaatctgaa ttagtttctc tatatcttct ctctaagttt
    82861 actattacac cttcaaagta tgatttataa tttctagttc ctccaaattt tgactcataa
    82921 gtaaactcta caattatatt atctttacca taaagtaatt cctccacaaa atcatctggt
    82981 aaatctttaa acggtgttgt caaagataca ttaaaatgct ctgctaaact ttttatcatt
    83041 ttactgtaat aagtgtcatc attaacacct gttgaacccc atgctgccac tgcaccttgt
    83101 tttattgata aatctttgtt tggtactact aattctgggt caacttcttt actttcccca
    83161 agaccattac aaacatcaca agcaccaaag ggagcattaa atgaaaacat tcttggagat
    83221 agttcttcta tgccaattcc atgttctggg catgcaaatt tagttgaaaa aagaatttct
    83281 tctccatcta ttatttgggc tattacaaga ccatcagata acttaactgc agtttcaatt
    83341 gaatctgcta gtcttgattc tattccatct ttaattacta ttctgtccac tacaacttct
    83401 atattatgtt tttgattttt atttaagatt atatcatcac taacttcata gttttcacca
    83461 ttaactctaa ctcttacaaa tccttctttt ttgatattat caaagacttt tttatgtgta
    83521 cctttttgtc ctctaacaat tggtgaaaga atttgtaatt tcgttctatc tggaaaatct
    83581 agcattttat caactatttc ttgaatagtc atctgactta ttttttcacc acatgtaggg
    83641 caatacacat ctcctactct tgcaaataat agtctcaaat aatcatatat ttctgttact
    83701 gttccaaccg tagaacgtgg gttttttgag gtagtcttct ggtcaattga gatagctggt
    83761 gataaaccct ctatatattc tacatttggc ttttccatct gacctaaaaa ttgtcttgca
    83821 taagcagata gactttctac atatcttctc tgcccttctg catatattgt atcaaatgcc
    83881 aaagaagact tcccagaccc tgataaacct gtaaaaacaa taaatttatc tcttggtaat
    83941 tctaaatcta tattttttaa attatgttct tttgctcctc ttattataat tttatcttcc
    84001 ataatgttgt ttttaccttt ctatttttcc aatttttctt ctaattgttt cactttatct
    84061 ctaagttctg ctgctctttc aaattgtaag ttttgagcag cttccatcat ctctgatttt
    84121 aatttatcta tattagctct tatttcgtct ttatcatctg tatcagatat tccatataca
    84181 acttcttcct ctgcaacctt agtagcttct atactatctc ttatattttt ctgtatagtt
    84241 ttaggaatga tattgtgctc ttcattatat aaattttgta tatctcttct tctctttgtt
    84301 tcatcaatag cattttgcat tgagtcagtt atcctgtctg catacataat tactcttcca
    84361 ttttcatttc tagctgcacg tccaatagtc tgtattaaag ctgtttcgga acgtaagaaa
    84421 ccttctttgt ctgcatctaa aattgctata agtgaaactt ctggtatatc caatccttct
    84481 ctaagtaagt ttattcctac aaggacatca aacttcccta atcttaaatc tcttattatt
    84541 tctgttcttt ctaatgtaac tatatctgag tgtaaatatt taacttttat ccctatttct
    84601 ttaagatagt tagtcaagtc ctcactcatt tttttagtta aagtggttat aagcactctt
    84661 tcattttttt ctatagtttt agttatttct cctaccaaat catctatttg attatttata
    84721 ggtcttactt ctacaattgg gtctaaaagg ccagttggtc ttattatctg ttctgctatt
    84781 gtctctgagt gttgaatttc ataaggccct ggtgtagcac ttacaaacaa catttgattt
    84841 atatttcctt caaattcttg aaaatttaat ggtctattgt caagtgcaga aggtaatcta
    84901 aaaccattct ctataagtga tgtttttcta gacctgtctc ctgcatacat acctcttact
    84961 tgtggtatag tgacatgtgc ctcatctaca attatcaaat aatcatctgg gaagaaatcc
    85021 attaaagtat atggtctttc tccttctgac cttcctgtaa tatgtctaga atagttctct
    85081 atcccctgac aaaatcctat ttcctttaac atttctatat catacttagt tctttgttca
    85141 attctttgtg cttctaatag cctatcattt tccttgaaaa atttaatttg ttcttgaagc
    85201 tcgttttcta tttctacaat tgccttttca attctttctg gcgttgttac atagtgagat
    85261 gctgggaata taacaacatg atttctagta ccaacaatct tccctgttac ataatcaatt
    85321 tctgttattc tatctacctc atcaccaaaa aattctattc ttattgcttt ttcatcatta
    85381 ctagctggga atatttctag tatatctcct ctaactctaa aggtacctcg tgtaaagttt
    85441 atgtcatttc tttcgtattg tatctctatt aaccttttta ttacatcatc tctgtctctt
    85501 tgcatacctg gtcttataga taacataagt tctttatagt cttttgggtc accaagtcca
    85561 tatatacaag atacagatga tattattata gtatctcttc tctctaaaat agatgctgta
    85621 gcagaatgtc gaagtttatc tatttcatca tttatgcttg catctttttc tatataagtg
    85681 tcactgtgag ctacatatgc ttctggttga taataatcat agtaacttac aaagtattca
    85741 actgcattgt ctgggaaaaa ttctttaaac tcactgtaaa gttgtgctgc tagagtttta
    85801 ttatgagcca ttattagggt aggtttctta acttgttgaa tgatatttgc cattgtgaat
    85861 gtttttcctg aacccgttac acctaataat gtcgaaaatt tctcatttct atttatagaa
    85921 tccactattg atttaatagc ttctggttgg tcacctgtag gtttgaagtc tgattttatt
    85981 ttaaaatcca tagtatcact ccttttttcg aacattagtt cctgacatgt attattatat
    86041 cataccttta taaaattgtt taagttattt taaatatttt acccatttaa attttactta
    86101 aatctaaaac ataaaaaaag acttctatct ttgcaataga agtctataat tcatggaaaa
    86161 taaatacttt attcaactta cataataaaa tcaaatgctt ctttttcaac tccatctaat
    86221 tttaaattaa acatttttaa tatagttgct gcatgtgctc tcaggtctcc ttcttctata
    86281 accacaccac ctttaatatt tggtccaaaa gctataaatg gaggtctatt acctttagta
    86341 ggtaaatgac catgtgtcgc aacagcaaac ttgtaatctt ttatatctga atcttttata
    86401 atacttcctt cgaaatcatt tccaaatgaa attccttcaa gaccttcaat taccatttca
    86461 aaatcacctt caagaccaag gttagatgct tcctctttgg taaaaatatt ctctatgaat
    86521 aattcttctt taaattcttc aagcagttct cttaattctg ttaacctatc taagttattt
    86581 actattattt gagctgatat tccagaagaa tttacatagg catcaaaatc cttaacttct
    86641 ccattttcaa cttttataaa tccatttgat ttaagcaaga catttggatt tataacttta
    86701 tcaactctaa gatgaccatg gtccccaagt attataaaat ttgtatcttt gtatgtacct
    86761 gcatcctttg tagcctgaat aatatctcct aaccatttat cattcatttt taatgcttta
    86821 tctacttcgt cattaaataa gccataattg tgtcgtgtat ggtccaatgt agctaaatgt
    86881 attagcatta aatctggttt atattccttt attatatcta ctgaacagtt tactccaaat
    86941 aaatccatat tgggttctaa tttccaatcg atataatgac aatgtctatt gtaaataccg
    87001 tccattataa ccttactgga acttcgttca aaagtttctc ttgggtcatc ttctctacta
    87061 ggagcccata tttctgcaat attataatct atatttggat ttgctcccat tacaggccat
    87121 aatacactag aggtagttag attattttct tttgctacat ctataatagt ttttgctttt
    87181 atgtcctttg agtaccagta ccagtcttta ttttctttat ttgggtcaaa tttttcatta
    87241 tgatatatac catgtttttt aggatatact cctgtaacca tactagtatg acacggatat
    87301 gtcagagtgg gataaacaca atttatattt tttactatgg agcaattttc tagtatagat
    87361 ttaaagtttg gtagattttt tacaaattcc aagtcttttt caaataaaga atctactgat
    87421 aatactataa cctttgtcat ttacattacc tcttcattta tatattattg aatactttta
    87481 atcaacttac aagtctatat aattatttat ttgttttatc tattactgct tgcattgcag
    87541 aagttccttt gcttactaca tcttttggat ttgcatttaa atcagtaagc ccctttttaa
    87601 tctcatccat tagcttatca gttgcttcct tgtatccatt taacattgga agtgcaacag
    87661 catattgtaa ttggtcagtt gcaaccttat attgaggttt ttctttatat aatgtttgta
    87721 acttttctga gtttgttgca tcttcagttg ttggtaagta tcctgttgat gaactaaact
    87781 gaacgatttt atctgtttgt gtcatccatt ctataaattc tgcacttgct tctttttctt
    87841 tatctgttcc accttcaagc ataactagat tagctccacc tgtaggagtt gcgtattttt
    87901 gattttttgg taagaatgct gttgatattt caaacttacc ttctgtttgt tgcataagtc
    87961 ctgctaaatc tgctgttgat gtaaatatca tacctacttt accatttaca aaatcttgtt
    88021 ttgctacgtc ccaggcatta taatcttctc caggaggcat tttcattatt ccagctttca
    88081 tcatatcttg ccagaactta actggtgctt gtcctgcctc attattaaat gctacttttt
    88141 taccattagt tatttctcct ccactttgca ttaccatagc ttcatagaac caaatatcta
    88201 ttggcgtttc aaaaccataa gtatcttctg atttatttgt cattttacta gcataatcct
    88261 taagctcttc ccatgatttt ggacctgaag ggtcaagacc tgctttagtt gccatatctt
    88321 tatttatgta taatattggt gtacttctat taaatggcat accaacaaac ttatcctcat
    88381 aatatgaatt tgttaaaaga ccagaaaaga atctttcttg atatgtttta tcaaaatatt
    88441 tactcatatc tactaaagca cctgctgatg cgaatgtacc tgtttgagtt atttctaata
    88501 atgttacatc tggttggtct ttagatgtta aggctgcctg tagttttgtt gcattttcat
    88561 agtaatcttt ctgttttaac atatttactt gtacatcctt atgttcacta ttaaattggt
    88621 caaccatttt ttgagcagtt tctccattag ttccacctaa aggagcccag aaatctatct
    88681 tcgtaatatt gctatcagtc ttttctcctt tagatgcatt tgcatcatta gaatttgaac
    88741 acccaactaa agaacctaat aataatgtta ttgctaatcc tgttgctaat tttcttttaa
    88801 gcttcatttt catcctccaa atttagtctt taatttttta ttttattcct gaatacataa
    88861 aagcttcttt tactttttta ttcgcaaata tataaacaag aattattggt aaaattagaa
    88921 ttatatttcc tgccattatt acattccatg cagtaattcc ttctgaagat ttaaggagcg
    88981 caactcctat tggtaaagtt cttattgcat ctgtatttgt cattactaag ggccagaaat
    89041 aattattcca atggtatata aaattaaata atataaatgt tattactgct ggttttgcca
    89101 taggaaccat tattctaaac attattttta agtcagaagc attatctagt ttagctgctt
    89161 caataatttc atctgaaatc tgcataaaat tctgtctaag cagaaatatc ccaaatgaag
    89221 atgtcataaa tggaattata agtggaacat aagtatttat aaatccaaac ttactcatca
    89281 tcatatagac tggtagaaaa gttacttgtg gtggaatcat aagtcctaca agcaaaagcc
    89341 caaatagcat tttttcacct ttaaatttaa tcttagaaaa tgcatatgct gctggaacca
    89401 taactaaaaa ttgtacaatt aaaacacttc ctgttacaaa tacactgttt agcaaatatc
    89461 taaaaaatgg acctgaatgc catgctgtac tataattttc taacactata tcatttggta
    89521 taatagtggg cggtaattgc atcatttctt gtggattttt aaaggatgta gatagcatcc
    89581 atataaaagg gaatataaat attgcagata aaagtaatag aattaatatt tctattgatt
    89641 ttgatgctat tttttttata tcaactttgt cttttttgat aggttgtata atatccattg
    89701 tctcctcctt ttggataaac cttataatat ctactttttc tttattgata atgaactttc
    89761 tttgataaga gtctaaagtt agcatatgta gcaatcataa ctatagctaa caaaaacata
    89821 ctcgctgcac tcgcttgtcc tatgtcatag tatataaatc cagactcata tatataataa
    89881 gataacatgt tagttgaacc cactggccca ccttgtgtca ttatacttat ctcatcaaat
    89941 acttgaaaag atgcagttgt tgttgttatc ataagaaaaa atattgttgg tgataatagc
    90001 ggcaatgtca ctttaaagaa attcgttatt ttgtttgcat tatctaattt agatgcttca
    90061 tatatatagt ttggtatgga tttaagacct gataacacta ttaagatatt gtatccaaga
    90121 gtcttccata ttcctattag aattagtgac attaaagctg aactttcact tgaaagccac
    90181 tttgatgttg gtaaatgtaa tgtcgatagt atccagttta aaaagccata ttgtggctcc
    90241 ataatccaaa gccacaatac agctacagat actagtgaaa ttacatgtgg tgtaaagatt
    90301 atactttgcg ttaaattgtg tattatagaa tttttattta gccaaacagc aagtactatg
    90361 gctagtgata tagatatagt caccataaaa aaagtataaa ttgcagtgtt cgttatagtt
    90421 tgtataaact ctggattctt taaaagttct gtataattgt ttaagcctac attttcaaag
    90481 gttgaactaa gaagactacc agatttgaag cttagagata tcgtcttaat tattggatag
    90541 actataaaca ataaagttcc tataatagca ggtgttatat ataaatatgc tttattaaat
    90601 tgctttttca taaatatctc ctttaatttt agaatacata cttttacata cttttacatc
    90661 ttctattctt tctccatttt tatcaaaata atataaatct ttttctaata cacttattct
    90721 taaagttctt ccaatatcaa atatatcatt ttccgttttg actcttatct ctctattaag
    90781 aacagataaa caatatatag tttcatttcc caacacttct ttagtcacaa cttttccaaa
    90841 taggctaaat acatcatcat ttttcccatc tatgtttata ctctctagtg ttgctttttc
    90901 tggcctgaat ccaaatttaa tatcattttt tatatctagt atgttcatac aaggactccc
    90961 tataaattga gctacaaata aatttttagg attttgatat atcttctttg gggaacctat
    91021 ttgcattatc tttcctttgt tcattataac tatcttatct gccattgaca ttgcctcaac
    91081 ctggtcatga gttacataaa caaatgtaga ctttagtttt tgatgtaatt gtattaattc
    91141 tgttctcatt tgatttctta atttagcatc taaatttgac aatggttcat ccattaaaaa
    91201 tacctttgga tttttaacca tagctctagc aagtgccact ctttgtcttt gacctccaga
    91261 taagttgcct ggtttcacat ttaaatattc tctcaatcct actacatcaa taacttctgt
    91321 tataagtttt tctctttttt ctttttctac ttttttgttt ttaagaccaa attctatatt
    91381 ttctctaaca gtcatatgtg gatataatgc ataattttga aataccatag ctatatccct
    91441 tttactagca tccgtttttg taacatcatt ttcttcaatc ttcaacctac ctgaagttat
    91501 atcttcaaga cctgcaatca ttcttaacgt agtagattta ccacatccac ttgggcctac
    91561 caatactgta aaagaaccat cttctatagt aagatttaaa ttttctataa ctgtattttt
    91621 tttattataa gattttgtta cattttctag tcttatatct gccattttta tcctcccaaa
    91681 atatttaatt taattctacg atttacttat agattaataa tactcttaat ttgtaaagga
    91741 tatattaaga caaagtgaat atatggtaaa cttattaaaa taaaggttaa attataaaca
    91801 ttttttgtta aatatactga ggttaagatg atatacagct tatgttagct ataattttct
    91861 atatttttca tattcatggt ttgctatcac aatgtaattt taatttatga agagtcatat
    91921 aaaactattt attttttata taatttgatt tgaaagtaaa aaagacctct atgggggata
    91981 gaggtcttta agtgggggag attgagtaat ttatttttgc tatattaagt tttacaatta
    92041 tattatgtcc caaatttaca aggtttaaac ataaaaaata aaactttttt aatttttata
    92101 aatttaccc
//
LOCUS       DAMJYC010000004        90572 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJYC010000004 DAMJYC010000000
VERSION     DAMJYC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 90572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.004"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(72..845)
                     /locus_tag="PC472_000255"
     CDS             complement(72..845)
                     /locus_tag="PC472_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2725631.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1231)
                     /locus_tag="PC472_000256"
     CDS             complement(845..1231)
                     /locus_tag="PC472_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725632.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="PC472_000257"
     CDS             complement(1256..1573)
                     /locus_tag="PC472_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2725633.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="PC472_000258"
     CDS             complement(1585..1845)
                     /locus_tag="PC472_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2725634.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="PC472_000259"
     CDS             complement(1845..2141)
                     /locus_tag="PC472_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2725635.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="PC472_000260"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="PC472_000260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2725636.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="PC472_000261"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="PC472_000261"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2725637.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="PC472_000262"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="PC472_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2725638.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="PC472_000263"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="PC472_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2725639.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8034..9140)
                     /locus_tag="PC472_000264"
     CDS             complement(8034..9140)
                     /locus_tag="PC472_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2725640.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="PC472_000265"
     CDS             complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="PC472_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2725641.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="PC472_000266"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="PC472_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2725642.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="PC472_000267"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="PC472_000267"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2725643.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="PC472_000268"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="PC472_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2725644.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="PC472_000269"
     CDS             12234..12941
                     /locus_tag="PC472_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2725645.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /locus_tag="PC472_000270"
     CDS             12910..13539
                     /locus_tag="PC472_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2725646.1"
                     /translation="MRKSLKLIKSKSKEEAISKVIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="PC472_000271"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="PC472_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2725647.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15125..17020
                     /gene="mnmG"
                     /locus_tag="PC472_000272"
     CDS             15125..17020
                     /gene="mnmG"
                     /locus_tag="PC472_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2725648.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTNSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="PC472_000273"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="PC472_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2725649.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17895..18680
                     /gene="noc"
                     /locus_tag="PC472_000274"
     CDS             17895..18680
                     /gene="noc"
                     /locus_tag="PC472_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2725650.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18815..19588
                     /locus_tag="PC472_000275"
     CDS             18815..19588
                     /locus_tag="PC472_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2725651.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19590..20453
                     /locus_tag="PC472_000276"
     CDS             19590..20453
                     /locus_tag="PC472_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2725652.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20486..21628
                     /locus_tag="PC472_000277"
     CDS             20486..21628
                     /locus_tag="PC472_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2725653.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(22053..22952)
                     /locus_tag="PC472_000278"
     CDS             complement(22053..22952)
                     /locus_tag="PC472_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2725654.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLEIDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEINRISRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQEHPNIEIHILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEVEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGIAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSQYDSFA
                     VQKMKEHIVSYTKEKGFFNFKSF"
     gene            23222..24418
                     /locus_tag="PC472_000279"
     CDS             23222..24418
                     /locus_tag="PC472_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2725655.1"
                     /translation="MVKFAKRMENMKNSANIVRNLFNSMTDPEIISFGGGAPAIEALP
                     VDIVREISNEVLAKNKRGIEALQYSHPYGLKDLREAVAQKLLEPKGVHASPEDIMIVN
                     GGLETMNLICQVFIDPGDVILVESPTFVHCVEIFEMFQAKCIAVDMDEHGIIPEDIER
                     KIQKHHPKMVYVIPTFHNPTGRTLTTERRKILAGIGSKYDVLILEDDPYRDLRYSGEE
                     LPPIKAFDQTGHTILANSFSKIFSPGARLGYVLAEHAIIERLLDAKTATNSHTSAISQ
                     VLCAEFFNRGYFDEHLKNIRDIYRERRDVMIESIDKYLPKGTKRVFPDGGLFTWVELP
                     ENINTTELLKEAVEHKVAFVAGEGFFTEGGGKGRNCMRISFGNVPPEKIRIGMERLGQ
                     LIASKM"
     gene            24486..25232
                     /locus_tag="PC472_000280"
     CDS             24486..25232
                     /locus_tag="PC472_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2725656.1"
                     /translation="MTNKVPNLWNQLENLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSD
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNVSGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            25265..25768
                     /locus_tag="PC472_000281"
     CDS             25265..25768
                     /locus_tag="PC472_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2725657.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            26016..26851
                     /gene="panB"
                     /locus_tag="PC472_000282"
                     /pseudo
     CDS             26016..26851
                     /gene="panB"
                     /locus_tag="PC472_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            complement(28369..28944)
                     /locus_tag="PC472_000283"
     CDS             complement(28369..28944)
                     /locus_tag="PC472_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2725658.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            29204..30496
                     /locus_tag="PC472_000284"
     CDS             29204..30496
                     /locus_tag="PC472_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2725659.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            30601..31203
                     /gene="yedF"
                     /locus_tag="PC472_000285"
     CDS             30601..31203
                     /gene="yedF"
                     /locus_tag="PC472_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2725660.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            31276..31539
                     /locus_tag="PC472_000286"
     CDS             31276..31539
                     /locus_tag="PC472_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2725661.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            31554..31739
                     /locus_tag="PC472_000287"
     CDS             31554..31739
                     /locus_tag="PC472_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2725662.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            31842..33038
                     /locus_tag="PC472_000288"
     CDS             31842..33038
                     /locus_tag="PC472_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2725663.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            33252..33530
                     /locus_tag="PC472_000289"
     CDS             33252..33530
                     /locus_tag="PC472_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2725664.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            33567..34001
                     /gene="ssb"
                     /locus_tag="PC472_000290"
     CDS             33567..34001
                     /gene="ssb"
                     /locus_tag="PC472_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2725665.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            34020..34247
                     /locus_tag="PC472_000291"
     CDS             34020..34247
                     /locus_tag="PC472_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2725666.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            34390..34710
                     /locus_tag="PC472_000292"
     CDS             34390..34710
                     /locus_tag="PC472_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2725667.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            34718..35674
                     /locus_tag="PC472_000293"
     CDS             34718..35674
                     /locus_tag="PC472_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2725668.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            35685..37679
                     /locus_tag="PC472_000294"
     CDS             35685..37679
                     /locus_tag="PC472_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2725669.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            37680..38129
                     /locus_tag="PC472_000295"
     CDS             37680..38129
                     /locus_tag="PC472_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2725670.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            38148..39476
                     /gene="dnaB"
                     /locus_tag="PC472_000296"
     CDS             38148..39476
                     /gene="dnaB"
                     /locus_tag="PC472_000296"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2725671.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            39681..39989
                     /locus_tag="PC472_000297"
     CDS             39681..39989
                     /locus_tag="PC472_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725672.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            40071..41360
                     /locus_tag="PC472_000298"
     CDS             40071..41360
                     /locus_tag="PC472_000298"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2725673.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            41686..41761
                     /locus_tag="PC472_000299"
     tRNA            41686..41761
                     /locus_tag="PC472_000299"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41719..41721,aa:Lys,seq:ttt)
     gene            41768..41842
                     /locus_tag="PC472_000300"
     tRNA            41768..41842
                     /locus_tag="PC472_000300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41801..41803,aa:Glu,seq:ttc)
     gene            41847..41922
                     /locus_tag="PC472_000301"
     tRNA            41847..41922
                     /locus_tag="PC472_000301"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41880..41882,aa:Val,seq:tac)
     gene            41956..42030
                     /locus_tag="PC472_000302"
     tRNA            41956..42030
                     /locus_tag="PC472_000302"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41988..41990,aa:Thr,seq:tgt)
     gene            complement(42413..43405)
                     /locus_tag="PC472_000303"
     CDS             complement(42413..43405)
                     /locus_tag="PC472_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725674.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(43414..44472)
                     /locus_tag="PC472_000304"
     CDS             complement(43414..44472)
                     /locus_tag="PC472_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2725675.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            44597..46060
                     /locus_tag="PC472_000305"
     CDS             44597..46060
                     /locus_tag="PC472_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2725676.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            46162..46959
                     /locus_tag="PC472_000306"
     CDS             46162..46959
                     /locus_tag="PC472_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2725677.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            46972..49074
                     /locus_tag="PC472_000307"
     CDS             46972..49074
                     /locus_tag="PC472_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2725678.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            49142..49621
                     /gene="rlmH"
                     /locus_tag="PC472_000308"
     CDS             49142..49621
                     /gene="rlmH"
                     /locus_tag="PC472_000308"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2725679.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            49770..50081
                     /locus_tag="PC472_000309"
     CDS             49770..50081
                     /locus_tag="PC472_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2725680.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            50129..50446
                     /locus_tag="PC472_000310"
     CDS             50129..50446
                     /locus_tag="PC472_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2725681.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            50659..51360
                     /locus_tag="PC472_000311"
     CDS             50659..51360
                     /locus_tag="PC472_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2725682.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            51584..52885
                     /locus_tag="PC472_000312"
     CDS             51584..52885
                     /locus_tag="PC472_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2725683.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            53022..54116
                     /locus_tag="PC472_000313"
     CDS             53022..54116
                     /locus_tag="PC472_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2725684.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            54118..55251
                     /locus_tag="PC472_000314"
     CDS             54118..55251
                     /locus_tag="PC472_000314"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2725685.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            55269..56378
                     /locus_tag="PC472_000315"
     CDS             55269..56378
                     /locus_tag="PC472_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2725686.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            56443..57183
                     /locus_tag="PC472_000316"
     CDS             56443..57183
                     /locus_tag="PC472_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2725687.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            57188..58180
                     /locus_tag="PC472_000317"
     CDS             57188..58180
                     /locus_tag="PC472_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2725688.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(58368..59249)
                     /locus_tag="PC472_000318"
     CDS             complement(58368..59249)
                     /locus_tag="PC472_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2725689.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            59522..60910
                     /locus_tag="PC472_000319"
     CDS             59522..60910
                     /locus_tag="PC472_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725690.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            60938..61891
                     /locus_tag="PC472_000320"
     CDS             60938..61891
                     /locus_tag="PC472_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2725691.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            61951..62175
                     /locus_tag="PC472_000321"
     CDS             61951..62175
                     /locus_tag="PC472_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725692.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            62168..62680
                     /locus_tag="PC472_000322"
     CDS             62168..62680
                     /locus_tag="PC472_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2725693.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            62712..63566
                     /locus_tag="PC472_000323"
     CDS             62712..63566
                     /locus_tag="PC472_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2725694.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            64100..64894
                     /locus_tag="PC472_000324"
     CDS             64100..64894
                     /locus_tag="PC472_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2725695.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            64998..65804
                     /locus_tag="PC472_000325"
     CDS             64998..65804
                     /locus_tag="PC472_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2725696.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            65866..66387
                     /locus_tag="PC472_000326"
     CDS             65866..66387
                     /locus_tag="PC472_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2725697.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            66802..67464
                     /locus_tag="PC472_000327"
                     /pseudo
     CDS             66802..67464
                     /locus_tag="PC472_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(67636..67875)
                     /locus_tag="PC472_000328"
     CDS             complement(67636..67875)
                     /locus_tag="PC472_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2725698.1"
                     /translation="MKKEYGYPTLKVICQASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKIIMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(67880..68299)
                     /locus_tag="PC472_000329"
     CDS             complement(67880..68299)
                     /locus_tag="PC472_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2725699.1"
                     /translation="MNPSSFEHIVRIQFNALMMTVIKCTVKNRNRQFARRSKREVLFC
                     ELSDTKNVECVTKDNYSCDYISFEVLNFTIQISNEKLAVALYKLSNKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTVLKERN"
     gene            complement(68598..69293)
                     /locus_tag="PC472_000330"
     CDS             complement(68598..69293)
                     /locus_tag="PC472_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725700.1"
                     /translation="MCTRFVYNGIDKIVGFNFDIDLSVWDHTVITEENRFYIGIKMPD
                     GMYHSFHGINKNGNVGTLLYVHGNENARYIESEDCITISELTEKFIKAEISFDKALDI
                     VKKKKIIYAPDATMQAMLSDKKGRVLIIEPGIGYRYEQENFSLITNYSILKPDITKPY
                     IVSGDTRYEVAKELLAGYDKDFSVNDAFKVLKSVSQKDLWATRVSFVYSTEKNKVYYV
                     LNNDFSNVFEFQF"
     gene            69471..70028
                     /locus_tag="PC472_000331"
     CDS             69471..70028
                     /locus_tag="PC472_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2725701.1"
                     /translation="MKTTKEAVIKATSDLADEKGLNNVSLKAVAQKLNIRTPSLYNHI
                     ESLDNLLLEVAHNGMRDMNERMMKIAVGKIGKEAIKLVSIEYLNYMIEHPGVYETIQW
                     AVWHGTEETATIFNNYLSLLTTLIQSCSLNKDKTLEILNMLTGIIHGYTTLQLGNAFS
                     ASDKVRFELAEAIDTLLVGIFQKYK"
     gene            complement(70303..70938)
                     /locus_tag="PC472_000332"
     CDS             complement(70303..70938)
                     /locus_tag="PC472_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725702.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNFPTFDTLEKIMDVLGVMPNELLSGEWKYVNQSEKEVCQFLRIEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIVASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLKNNHFDDEDE"
     gene            71395..72486
                     /locus_tag="PC472_000333"
     CDS             71395..72486
                     /locus_tag="PC472_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2725703.1"
                     /translation="MTTHKNLSVKIDYISIVFDTATAEDVIMHILGLPTDIFNVYPAS
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFKHCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKL
                     QVPKQQNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSS
                     KLTAHLQMINRTDLIPYIQYDTKHGKGGI"
     gene            72486..72689
                     /locus_tag="PC472_000334"
     CDS             72486..72689
                     /locus_tag="PC472_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2725704.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEQVIDKLNAI"
     gene            72768..73961
                     /locus_tag="PC472_000335"
     CDS             72768..73961
                     /locus_tag="PC472_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2725705.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYFDDVLI
                     LLKTGLRISELCGLTIMDVDFNHEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSE
                     ETIKAFQRLMKKRPKAEPFVIDGRGNFLFVNQKGKPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            74136..75572
                     /locus_tag="PC472_000336"
                     /pseudo
     CDS             74136..75572
                     /locus_tag="PC472_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            75587..75874
                     /locus_tag="PC472_000337"
     CDS             75587..75874
                     /locus_tag="PC472_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2725706.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            75901..77172
                     /locus_tag="PC472_000338"
     CDS             75901..77172
                     /locus_tag="PC472_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2725707.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            77230..77829
                     /locus_tag="PC472_000339"
     CDS             77230..77829
                     /locus_tag="PC472_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2725708.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            77834..78739
                     /gene="dapA"
                     /locus_tag="PC472_000340"
     CDS             77834..78739
                     /gene="dapA"
                     /locus_tag="PC472_000340"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2725709.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            78845..79477
                     /locus_tag="PC472_000341"
     CDS             78845..79477
                     /locus_tag="PC472_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2725710.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVRKI"
     gene            79954..82461
                     /locus_tag="PC472_000342"
     CDS             79954..82461
                     /locus_tag="PC472_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2725711.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            82803..84965
                     /locus_tag="PC472_000343"
     CDS             82803..84965
                     /locus_tag="PC472_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2725712.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            84970..85587
                     /locus_tag="PC472_000344"
     CDS             84970..85587
                     /locus_tag="PC472_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725713.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            85776..86120
                     /locus_tag="PC472_000345"
     CDS             85776..86120
                     /locus_tag="PC472_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2725714.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            86690..87556
                     /locus_tag="PC472_000346"
     CDS             86690..87556
                     /locus_tag="PC472_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2725715.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            87669..88073
                     /locus_tag="PC472_000347"
     CDS             87669..88073
                     /locus_tag="PC472_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2725716.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            88237..88686
                     /locus_tag="PC472_000348"
     CDS             88237..88686
                     /locus_tag="PC472_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2725717.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(88842..89219)
                     /locus_tag="PC472_000349"
     CDS             complement(88842..89219)
                     /locus_tag="PC472_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2725718.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            89481..90398
                     /locus_tag="PC472_000350"
     CDS             89481..90398
                     /locus_tag="PC472_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2725719.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        34262 a        11182 c        15104 g        30024 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 actttatctc taaaatactt ttttatttct cctactatag ttggtgttgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatatta aatacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttctt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat catccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttaacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctatagcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcatccttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatctcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 gcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aaccagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagc tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctctcagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc agtatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaagtt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctaataaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccgtctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 tccccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttcg tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atgtattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggcaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcctca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccttt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatatat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat ttcaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtat tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagta tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aatttttgta
     8461 tcattaatta taaaaatagc atttttttcg ttaaatccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caaccgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta ggatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatga acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctgagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt gctattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggcc gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag acttttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagtta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag aaggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg cggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta caaatagctt agttgagctt ggtcttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag tacgatttag tgataaaaca tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggtttctaaa taaaaaggct gctgtagata aagaatttga
    16561 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaagg cgctggagat gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa actagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgactgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttat tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaaaatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
    17761 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
    17821 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
    17881 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
    17941 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
    18001 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
    18061 aactaattgc aggagaaaga agattaagag cagctaaatt agcagagctt aaaaccatac
    18121 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
    18181 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
    18241 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
    18301 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
    18361 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
    18421 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
    18481 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
    18541 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
    18601 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
    18661 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
    18721 cttgtaatat aaattgattt atagacatta tttatactta aaaaacacct attcaacatt
    18781 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
    18841 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
    18901 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
    18961 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
    19021 tattaaagaa gcaataataa gtactgaatt tgagaatgta gatgtagttc catcagctac
    19081 tgaactatca ggtgcagaaa tagagcttac atctaaaact aatagagagt atattttaaa
    19141 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
    19201 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
    19261 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactatca aattagtaaa
    19321 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
    19381 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
    19441 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
    19501 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
    19561 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
    19621 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
    19681 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
    19741 atgaagaaaa gataaaagta ttgtctgatt ctataaaaaa ttatggtgtt ttacagccaa
    19801 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
    19861 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
    19921 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
    19981 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
    20041 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
    20101 gacaagatgt tatagaaatg atagacagtg gtagaattac agcaggccat ggtaaggctt
    20161 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
    20221 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
    20281 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
    20341 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
    20401 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
    20461 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
    20521 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
    20581 aggtagagca ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
    20641 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
    20701 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
    20761 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
    20821 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
    20881 atcaataaag aataatacta aattgattgt aacaacacat gcgtctaatg tatgtggaac
    20941 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
    21001 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
    21061 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
    21121 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    21181 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    21241 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    21301 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    21361 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    21421 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    21481 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    21541 agtaaggttt agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    21601 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaatg
    21661 gggatgtttc aaaataaaaa tttgaataga gtattgaaat ttttatgtat attctttaaa
    21721 taaaaacttc aatactctat taattaaatt actaattttg ctaattttca taaaatctgt
    21781 taaattactc ataaagttat atattttcaa aggctcaagt cttgtatgaa attacttgtt
    21841 ttaatttttt atataactat gacttttgca atctttttag ttgaaaagcg ccttcaatca
    21901 tactaaaata ataatattac aatgctaaat tattttatcc tctattataa ttataaatac
    21961 aaaccggtgt gactcatctt atgaaaaaag tattgttcag cgttgataaa actgacaatt
    22021 aagtaataaa aatattatac taattttact ttttaaaaag acttaaaatt aaaaaaaccc
    22081 ttttcttttg tataacttac aatatgttct ttcatttttt gaactgcaaa agaatcatat
    22141 tgtgagttgg gataagctaa actaattaca cggtaatgct ctggtacaaa tgggtatgta
    22201 tcaacattgc caacacaact tttataaatt agttctgata aaatagcaat tccaaaccct
    22261 gcttctacca tggcaacaat agcactatca gtatctatgt gaaattgaga gtacacatta
    22321 agatgatatt cacgaagaat tttttctact tcctggtcat aatctttttt aggcataaca
    22381 aaggacatat tttggatatc ctctattgta acatacccag gattacaagg tacaaatcct
    22441 tttggaaaga cacaaatgat acggtctttg tgaagcggaa tagattcaat atgtggagga
    22501 agtactgtgt tggatataaa agctaaatcc acaatagaag tttcaatcca ttctaggata
    22561 ttaatatagt tcccttgaag aatatgtatt tcaatattag gatgttcctg ccgaaatgat
    22621 ttcataatct cagggatcca gtttattgta atactattaa aagttccaat acgaataata
    22681 ccactttctg aacgtgaaat acgattaatt tcttgttcta aaatttcgtt actatgtaat
    22741 aaaatacgaa tatgaggtaa aattttttca gcttctcttg ttaaactaat accatttcta
    22801 tttctaataa acaattgaaa acctacatct atttctaatt ttgcaatcgc atgacttaca
    22861 gctgacggag ttaagtttaa aatacttgct gcttttataa aactattttg ctctaaaact
    22921 gtttcaaaaa tagtatatcc ataaattgac atatgattta ctcctttcct ttagatgagt
    22981 aaaaactcat ctaaaacgat aaaaagattc gctttatttt ataataataa tctactatta
    23041 ttaaattata aatacaagtg atcatttgca ttttttactc aagataataa aaatagtacc
    23101 acccaaaata caagaattaa aatattattt ctgtttaaaa gtgagcggaa acggaacctt
    23161 tgctcatcta atctcatata attacagaca aatatcaact tgataaaaag gaggaactaa
    23221 tatggtaaaa tttgcaaaaa gaatggaaaa tatgaaaaat tcagcaaata ttgttcgtaa
    23281 tctttttaat tccatgacag atcctgaaat tatatctttt ggcggaggtg caccagcaat
    23341 agaggctttg ccagttgaca ttgtaagaga aatttctaat gaagttttag caaaaaacaa
    23401 aagagggatt gaagccttac agtacagtca cccatatggt ttaaaagatt tacgtgaagc
    23461 agttgctcag aaattattag aaccaaaggg agtacatgca agtccagagg acattatgat
    23521 tgtaaatggt ggtttggaaa caatgaatct gatttgtcag gtattcattg atccaggaga
    23581 tgtcatttta gtagaatcac caacatttgt acattgtgtt gaaatttttg aaatgtttca
    23641 ggcaaaatgc atagcagtag atatggatga acatggaatc attccagaag atatagaaag
    23701 aaagattcaa aaacatcacc caaaaatggt ttatgtaatt cctacttttc ataatccaac
    23761 tggcaggaca ttgactacag aacgaagaaa aatattggca ggtattggta gtaagtatga
    23821 tgttttgatt ttagaagatg acccttatcg tgatttgaga tatagtggag aggagttacc
    23881 tcctattaaa gcatttgatc aaacaggaca tactatttta gcaaatagtt tttctaaaat
    23941 cttttctcca ggagcaagac ttggttatgt tttagctgaa catgcaatca ttgaacgatt
    24001 acttgatgca aaaacagcaa ctaattctca tacatcagct atttctcaag tattgtgtgc
    24061 agagtttttc aatcgtggtt actttgatga acacttgaag aatattcgag atatttatag
    24121 agaacgcaga gatgtgatga ttgaaagcat tgataagtat ttaccaaaag gtactaaaag
    24181 ggtatttcca gatggtggtt tatttacttg ggtagagcta cctgaaaata tcaatacaac
    24241 tgaattattg aaagaagcgg tagaacataa agtagctttt gtcgcaggag aaggattctt
    24301 tacagaaggt ggtggaaaag ggcgaaattg tatgagaatt agctttggca atgtacctcc
    24361 tgaaaaaatt agaattggta tggaaaggct tggacaacta atcgcttcca aaatgtaaat
    24421 aattacttat ataaaataca actttatatt ttaagattta taatataacc aaaggaggaa
    24481 tacttatgac aaataaagta ccaaacttat ggaatcaatt ggaaaactta aaaaaatcat
    24541 ataaatgggt ggatttgagt cgtgaagtaa gtcctgagac acctcattgg acaggctttc
    24601 ctgcaatgga agtagaacct ttctacttat tggatagagg agatggattt aatgtaaata
    24661 aatatacact agtaactcaa tatggaacac atgtagatgc accaattcat tttgttgccg
    24721 atagaaagta tttggatgaa tatacgccaa tggatatggt acttccactt tgtgtcattg
    24781 ataaaagtga tgaggttgct aaaaattcag attatgtagt aactgttgaa gatattcaag
    24841 catgggaaaa agaatatggt aatattccag agggtgcatt tgtggcattt cgttcagatt
    24901 ggtcaaaaag acctgatgat tctttagata attatgattc agatggtaat aagcattttc
    24961 caggatggag tatagaagct ctgcaatata tggttgagaa gagaaatatt gctgcaattg
    25021 gacatgaaac ttgtgataca gatgcaccaa ttttatcaaa tgtaagtgga tatcaggcag
    25081 agacatatat tttaggagaa aatcgatatc aagttgaatt tttagtaaat ttgtctgagt
    25141 tacctgcgat aggagctatt atttttgtaa cattcccaag aatacacaaa gcatctggtt
    25201 ttactgcccg ttgttttgca atttgcccat aaggttttaa ctgttagaag ggagggggag
    25261 acagatgagt aaaaaagcga tgtttgtttg gtgtgttggc ttaggtctac tagatggcat
    25321 ctattgtatt ctttgttcct acttacccca tatacaaaac tatatgtgga ttggttttat
    25381 ttctcttcca atttactttt gtggaggtgc aaaattagaa gagatgccaa aatatttttg
    25441 ttgtgcagca agtggtatat tttggggtgg gctaacatta gttgtattag acataggatt
    25501 tatatccaat atacatttaa acatgcttgt gttcgtaaca attgttgtat gcattgcttg
    25561 ttttgtacat ctaggtgtat tgactgaaaa tgtatggaaa ggattgttct caagttgccc
    25621 aatgatgttt ggtggatttg cagcaatttt ttctcaagga ttagcggagt ggttttgggt
    25681 acttgtaaca cttacactag ggttgatttt tggttgtatc atggcttcaa tttcaacacc
    25741 tatcacaaaa tggattgata agaattgaaa taaagtagct attcaataat ttttaaaaag
    25801 ttaatttatt tatgaaatat aatggtatat tatttcaaga tggtttaaaa ggaaaagttt
    25861 tttaatactt ttccttttaa tttaaaatat aaatacattt gccacataag tgcttcttta
    25921 aaatctagta ttatctttac tagatatagg agtaagttgt tttaccaaat tagttttata
    25981 tgaagatatt tacaaaattt taggaggaaa aaacaatgaa aaatactata caaactttta
    26041 agaatgctaa aagtgaagga aaaaaactat ctatgcttac tgcttacgat tactcaatgg
    26101 caaaaataat ggatgagtgt aatataaatg gtcttttaat aggagattca ttaggaatgg
    26161 ttgtaaaagg cgaagaaaat actctctctg ttactataga tgaaattata tatcatacaa
    26221 aagctgtaaa aaatggggca aaaaatgcct taatagtaag tgatatgcct tttctatatc
    26281 ttaccatgtt tctatagaag atgctgttaa aaatgctggt agacttgtta aagaaggagg
    26341 agctcatgct gtaaaattgg agggtggagc aaatgttata aagcaaatag aaagcattgt
    26401 gaatgctcaa atacctgtaa tgggtcattt aggactaact cctcaatctg taaattcatt
    26461 tggtggattt aaggttcaag ggattactag tgaagcagca aaacaactta ttgaagatgc
    26521 taaattaata gaaaaagcgg gggcattttc tattgttctt gaaggtgttc ctgcgaaaat
    26581 atctgagata ataactaact ctatttcaat tcctacaata gggataggag caggaattaa
    26641 ttgtgatgga caaattttaa tttaccaaga tatgttaggt atatttgaag atttcgtacc
    26701 taagtttgtg aagcaatatg caaatgtagg agatataatg aaagattcaa ttaaaaatta
    26761 catactagaa gttgaaacag gggcatttcc acaagaaaaa catagttttt caataaaaga
    26821 atctgaatta gaaaaattat acgaagatta aggatgttat aagctgtcat atataacaat
    26881 ttattattat atttagagat ggattgtcca tcttttctgg ataaaaattt atccagtttt
    26941 atgattataa taaccaattg taaaatatat ctaaacaata tattatacta tgtagatttt
    27001 tcaatgaaat aacatcagat atttactgaa tatttttatt tcttattttt tagaggaact
    27061 tcttttaatt taaatataaa aataatgaat gtgtttatat agtaactgca tatattaata
    27121 gtttaaaagg acactattca ttcgtagaaa cttaaattga tgattttatt aaaaaatatg
    27181 tagaagatat tgtaggttta tgtcttcata gaataatgat tatattatat gaatgtgatt
    27241 tagcataaaa ttagatatat taaagttgag tgagaagaat cttgaacaaa gttcttctta
    27301 ccaactttaa tatatttcta ttatacaatc tattttttat cattattgaa attgtttaca
    27361 acttgattta actcaattaa tatacttcca acttccatat agagaatttt actataaaca
    27421 caagtaatac aataggaatg taattatcac ttgatttttt tattttttaa atcccataac
    27481 aaaacattaa gtgcaactat tggagttaac aattgttgta gtataaaagc tattaagtta
    27541 tctatatatt atcttcctaa aaaagttttc ttaacacttt aattattatt caaaaactat
    27601 ttaaacaaag ctataagaga gtttaagcta tcactccaat aagatttatt ctttatttta
    27661 tacatctaat atttcaaatt cgtaaattta cttccaatta tagaattata cttgtttcgt
    27721 atttattcaa tttatcttat aaaagctttt gttttgtaaa aatattaatt ttatattaaa
    27781 acaagactta gaaactatat acttcttgaa aaaattctaa tatagataaa tttaaatatt
    27841 ataaatgagg tattttttta aatgaggaag ataatggtag ataattaaaa atatattatt
    27901 ttatcatttg atatacattt atttattcaa aaactataaa agaacattaa cttttttaga
    27961 agtagaattt acaactaaag attgtaatat tgctattgaa tctaagcatt ataaaaaaca
    28021 ttggatacaa tttattggta tagcattttc aaaatgtcaa tatcacttgt tatattactg
    28081 taaattgtat agattatctt agccttgaaa gtatttgtgt atatatggtg actatgataa
    28141 ttgacaatct agtaaataag ataaagataa cattagtagg ataaaatatt ggtatataaa
    28201 agatctaaat agaacctgaa aggtgttgtt tcaaaattga ttttttataa tcaaattttt
    28261 atttctattt ttatgttttt gtatgtaaaa aagaaaggaa ggagataatt caaattttta
    28321 ttttgaacca tctccttcta tatgataatt tataattgtt tcaagaaatt actttaagta
    28381 atctccttct tcaagaacta tatgaccact ttcgtaatta gaaccatcaa cagtaatttt
    28441 tatttgttca acattcatat tttttatgta agtacgagca actgaatcaa gcattaaagt
    28501 ttctgcatca ctacctaagt ttgactttgt aaagtcagag ctaacatcta atataccagt
    28561 ttttacacca tcaatgtctt ttgtagtaaa tgaatttaat tttgcagatt ctggaacaac
    28621 tttaagtttt tgaagttctt caaataaagt gttctcatca attttattta aatcaacttt
    28681 attttcaatt aaatctgtct tatctacatc aaaagagtaa atagtagctg attgagtttt
    28741 agtttcttct tttggagtag gtttagcttt gttattagga acctcttttt cagtattgtt
    28801 ttcttctttt ggttgctcaa catttgtatt gctcttagtt tcttttttag atgaatcact
    28861 tccattttga gcattagtac aaccaactgc gaatatagat atagataata tacttaacac
    28921 taacattatt ttcttatttt tcataaaaat ttctcctctc ttaaaaacta ttcttatata
    28981 taattatagt cgttatgagc aaatatatga atacaaaata gtaacaaatt taaaaaatga
    29041 tttatttaaa ttaataaaac tcaaaataaa ctaaatttta gtcattttta tgggaaatca
    29101 tttaatgtac agtgaaaata ttgtaaaata aaagtagtgt ttttaaaata aatataagtt
    29161 ataattatat tataaaaaaa tgtcttcaaa aggtgggaat agtatggaaa ttttgagctt
    29221 aggagaaaag attaaaaaat taagaaaaga aaaaaacatg acattaaaag aattagctgg
    29281 agataggata acagcagcac aaataagcca tatagagagg gataaatctc atactagtta
    29341 tgaactgttg gaatatctag gcgataagct agatgtaagt gtagaatact tattagagac
    29401 caaggaaatg caatcaaaaa agataactga taatttaata ctacagagcg aaatttatat
    29461 aaaagaaaat gaattaaaca aggctgagga ccaaataaaa gaagttttag atatatgtaa
    29521 gagatacgat ttaatggaga actatggcag atgtaatttt ttaatggcta atattaattt
    29581 gaaaaaatct aattataatg atgctataga aaactttgaa aaggcattgt acttctttat
    29641 aaaaaatagt gatagtgaaa atatacttaa gtgttattta aacataggga aaatatatat
    29701 gcaggaagac ttttataaag gtgctataag ccactttaat tttgcagagg aaattttgtc
    29761 agagaataaa tttgaggatg ttaacataca caaggaacta tatagtaaga tggcatattg
    29821 ttacataaaa ctagataatc caaatatgtc attgaagtac atagaaaaga taaatgaaat
    29881 agataataga aatgatagaa aagaagatgt cgatattttg gtgttaaaag caaataatat
    29941 gcttgagatt ggaaaatatg aagaatcaag agattacttt aaaaaagcac taaaaatact
    30001 tgagagtgag gataataaaa ctggattagc aaatgtgtat ttaacaattt gtgatgttta
    30061 cagaaaaata ggtgaaacag atagagtact tgaatattca caaaaggtat atgacataaa
    30121 gaaaaatgat gaagatgaat acatgatgtc aggattattt aagataatag aagcatatat
    30181 agataaagaa gattatgatt tagctagaaa atattgtaag atagccttag cttcatcaat
    30241 aaaaaataaa aataaattca atgaatataa agcattgaga ttctatgcta atatgtataa
    30301 aaatcagaat gaagtaactc ttgcaattga atatctgatt aaatgcataa aaatagtatc
    30361 agatttaggc aacaatagaa tattggccaa tttatatata gacttaggtc aattatattc
    30421 aaatatatca aaagaaaaag agttagaata ttatcaaaaa ggtgtattta tgtacaaaaa
    30481 tttagaaata atgtagggta aataaaccta taagggtata gaaacaatct ataacaagca
    30541 agataatatc tatgataaaa tataaattgt atgaatattt ttaaggagat gaatcagaca
    30601 atgagtataa aaatagatgc gagagggctt gcttgtccta aaccagttat aaatacaaaa
    30661 agagaattgg ataacctaga agaaggtgtt gtagttacaa tagtcgataa tgaaacagct
    30721 aaggagaata tattaaaact tgctaaatct ttaagttgtg aagcaaatat tgttgacgaa
    30781 aaagagaatt ttatatctat agaaattaga aaaggtaaaa atatactaga taaacaaaat
    30841 gttgtagata ataaagaaag tgaattagac aatacatgta tatttatatc ttcagataag
    30901 atgggacttg gaaatgacga attaggaaaa gtgttaataa aaggatttat atatacatta
    30961 acagaatcta aaccttatcc taagtatgta cttttagtta atggaggagt aaaattaagt
    31021 gctgaaaatg aagaaaccat agaaaatcta aaaatacttg agaatatggg tgtagaagtt
    31081 ttatcttgtg gtacttgttt agattattat aatttaaaag acaaattaca agtaggtaca
    31141 gttacaaata tgtatactat agttgagaca ttaaagaatg catcaaatac catttctata
    31201 taatgtatgt ttgtttaata atataaaatt aataataaag attaactcaa atagatagtc
    31261 agaaaggatt aagtaatgaa tcaaatgtat atagtgtcat ttaattcgac acatcatgcg
    31321 ataagatcag aaaagttatt tggtgaaaat agtctaaaag taatggctct tccaacacca
    31381 agagaaataa cagcaagttg tggaatatca attaaatttt cttttgaaga tatggaaaag
    31441 ataaaaacta ttttagttga aaataatgta gatataaaag gaatttattg tataagtaaa
    31501 ttagaaaatg gctcaaaaga agtagagaag ttagattagg aggctaatta tttatgccaa
    31561 tggatttaaa aataggtgat attgttgaat taaaaaagca acatgcatgt gggtgtaaag
    31621 agtttgaaat cataagaacg ggaatggata taaagataaa atgtactaaa tgttcaaggc
    31681 taataatgct tgatagagag accttagaaa agagagtaaa aaagctaata aaaaagtgat
    31741 aaaaatttaa cacttaaaaa atgtcaaata ccgtatatta atatccaaaa ttttggtata
    31801 ataagaattg aagaaaacat tttcatgggg gtgctttaat tatggcagtt aaatatgcaa
    31861 aaagaatgca aggattacaa ggatcagaaa tacgtgagct tttaaaactt actcaacaac
    31921 cacaaataat atcttttgct ggtggaatgc cggctccaga gttatttcca gttgaggaaa
    31981 tgaaaaaagt atcagttgca gtgcttgaag aaaatggaag atcagctatg caatatacta
    32041 caacagaagg atatgaacca ttaagagaaa aaatagcagc tagaatgaat gataaaaaca
    32101 aaactaatgt aaacaaagat gatatattag ttacaagtgg ttctcaacaa ggtctagatt
    32161 ttgcaggaaa agtatttatt gatgagggtg atgtaatttt atgtgaaagt ccatcttaca
    32221 taggagctat aaacgcattt aaatcttatc aacctaaatt catagatgtt ccaacagatt
    32281 ctgatggaat gataatggaa gaattagaaa agatactaga aacaactgat agaataaaaa
    32341 tgatttatgt aatacctgac ttccaaaatc caacaggaag aacttggcca cttgaaagac
    32401 gtaaaaaatt catggaaata gtaaataaat ttgaaatacc agtaatagaa gataatccat
    32461 atggagattt aagatttgaa ggagagactt taccatcttt aaaatcaatg gatacaaaag
    32521 gattagtaat attcttagga actttctcta aaatattctg cccaggatac agattaggat
    32581 ggacttgtgc ttctccagaa attttatcta agtttaactt tgcaaaacaa ggtgcagact
    32641 tacaagcatc aactatatct caaatggaag taagcaaatt tatggatatg tatgacctag
    32701 atgcacatgt tgacaaaata aaagcagttt atgttaaacg tagagatgta atgcttaaga
    32761 ctatggaaga agagttccca gaaggattag tatttactca tccagaaggt ggattattta
    32821 catgggtaga gcttccaagt aatttaaatg ctaaagaatt aatgccaaaa tgtttagata
    32881 agaatgttgc ttatgttgct ggaggaggat tcttccctaa tggtggtaga gaaaatactt
    32941 tcagacttaa ctattcaaat atgccagaag aaaaaataat agaaggtata aaaaatatag
    33001 cagctgtatt aaaagaagct atgggtgtag aagcttaatt aaaaataata acttatatat
    33061 aatacaaggg atggttgttg ccatcccttg tttgtttgaa aagtatataa ttcaccttat
    33121 actaaacttt gtccaaaatt taaattgaca gaaataagaa taaatgataa aatgtgtagg
    33181 tatgagattt tagtctcatt tctctgctct aagagtgtag agccgttaag tccaaaggga
    33241 ggtgaattat agtgagaaat tatgaattag tttatgtggt aaagccaaac tctgatgaag
    33301 aagtaagaga ggctatacta aacaaagtta aggaagttgt tgcaactgac ggagaaatag
    33361 ttaaagttga tacttgggga actaaaaaat tagcttatcc aatagctaag tttacagaag
    33421 gtttctatgt attagttaac ttcaaatcag cagtagacgt tcctaaagag atagacagaa
    33481 acttaaagat aaatgaaaac gtaataagac acatgatagt tgttgcttaa taactggttt
    33541 tattataaaa aataggtggt gtttttatga atcaagttgt attagtggga aggttgacta
    33601 gagaccctga attaagatac atcccaggta caggaacacc agtggcatcc tttacaatag
    33661 ccattgatag agactatgta aaaaaagatg gatctaaaga aactgatttt atacctgttg
    33721 aagtaatggg taaatctgct gaattttgtg ctaactacat aactaaaggt agattagtag
    33781 cacttcaagg atctataaga gtagacaatt atcagacaca atcaggtgaa aagagaactt
    33841 ttacaaaagt aagtactaga tcagtacaag ctttggatag taagaataaa tcggaaaatt
    33901 catatagaga gagtgctcca gcttttgagc caagctttga acctcaaggt ttagacccac
    33961 aaggtttcca agctatagat gatgacgata taccatttta aaaaggaggg aaaaaagtca
    34021 tgatgaataa aaaaagacgt aagaaaaaaa gagtttgtca attttgtgct gacaaaaacg
    34081 ctaaaataga ttacaaaagt actcaaagat tacaaaagta tataactgaa agaggaaaga
    34141 tattaccaag aagaatatct ggaacttgtg ctaagcatca aagagaatta acagttgcta
    34201 taaaaagagc gagaaatata gcacttttac catatacatt agactaaaaa aatgagcctt
    34261 gtgctcattt tttattatat gtatttatat atctaaatga ggcgagctat aaagctcgtt
    34321 tttttgttta tatacttaaa tatattatta tttatgatat aatattatat attaaggagg
    34381 gttgtaaacg tgaaaataga taaaggttct gagataacga gaaacataaa gataatagaa
    34441 tggatgaaaa cagaaatatt aatgagtgta agtgatttgt ttaatttatt gtttaaaggg
    34501 gtaaaaccat tagatgaagc aattcaagat actttagcaa atataataat gataacatat
    34561 ctcttagcaa agagattagg aattagtttt agagatgtgg attataaagt caaggaaaaa
    34621 ataaaaattg gtatagatga ggaccatagc gttgaaagat ggtatggaga tttatctaat
    34681 ttaaaaaaac atatggataa tagggagtga tattaaattg aataataaaa ttagactatc
    34741 taaggcaatg tctataatag ttttagctat aataatcgct ttagtaatag cctatattcc
    34801 aatgctagga atatttagta tgttagcagc tgttccatat gtaattatag gtgctattac
    34861 tgatagaaag tattctctta tatctatttt ggttacattt tgtgtactga ttctatttgc
    34921 agacatttct tatgcattaa atatatgtat aatgtattct ttaccaggta tagtaatagg
    34981 aaagatgcta aagagaagct ttgaacaaga ggacagtaat aaattcgaac cgatatatgg
    35041 tggaaccata atatttgtac tttcaatgct tgtatatttt tttgtattaa agacattttt
    35101 aaatgtgaat ctattagatg aagtttctaa gatgatatca gaaattgtaa atattcaaaa
    35161 aaatagtttt tctgcaactg aacttaaagt gtttgataaa atgaagccag atgagattgt
    35221 aagttatttt acaaatatgc taccaatgat gttattttta caagggttgt tatcagcgtt
    35281 tgtcacatat tatttgagtg tattttttat aaaaaggatt acaaagatga atataagatt
    35341 tcctaagttt gctgattttt atttaccggg aaatgctata ctcacaactt tcttacttta
    35401 tttattagtc ttatttattc aaattattgg ttcaaaatta tatacagaat taataatgac
    35461 taatttacag ctagttttta atttaatgtt tgtaattcaa ggtatagcag tttgcattta
    35521 tttcttaaag aaatggataa gacagggacc aaataaaatt atgttttttt cagggataat
    35581 tttatgttta tttggattta tgggaatatc ttttgttggt atggtagata gtataattga
    35641 ctttagaaag gtaagaagct ataaatccac ttaggaggat agaaatgagt aataaacaaa
    35701 cctttaaatt aaatatgcca gaaataaatt tatatataat tgttattggt atttctagta
    35761 taattttgct ttattacaac ttgtatgtag ggtgtttatt tttctgtatt tttgtatata
    35821 tggtttttca caattggaga actactaata ttagacgtca tgaatggaca gagtatattc
    35881 aaaatttatc tttagatata gatgaaacaa ctaaaaaagc cataataaat cttccaatac
    35941 cattatgtat tttagaattt gatggaaata tatcctggta taatggaaag ttttatgata
    36001 tgattggtca aaaagattta cttgataaaa atatagagga tatagttaaa aatcttaatt
    36061 taagaaaagt attaaatgaa aataaagaga tgtacacaga aataaactat aaagaaaaag
    36121 aatatacaat aatttataat gttataaaaa atgatcaaga aaaaaatcct aaatatttga
    36181 tgatattata ctggatagac aagactgaat atttaaaagt aaaacaaaat tatgatgatg
    36241 aaaaaaatgc aatgatgcta atacaagtag atggatatga tgaagtatta aaaagtgcag
    36301 cagaagataa gagagcttta attaatgttg aagtagagaa aatactttct gctttagaat
    36361 taaattcaaa tggtgcattg agaagaactt caaaagacaa attcttcttg gtaatgcata
    36421 aaaaagagtt aaaaaaattg gaagctgaga agttttctat tttagataca ataagacata
    36481 ttgattatgg aaataatctt cctgttacta taagtatagg aattggtata gatggagata
    36541 cattaaatga gaacctaaaa cttgctacgg gtgctctcga tttagcactt ggtagaggtg
    36601 gtgaccaggc tgttgtaaaa acaaaagata aatttgtgtt ttatggagga aaatctaaag
    36661 ctgtagaaaa gaaaaccaaa gtaaaatcca gattaatagg tcatgcttta agagaggtta
    36721 tacaacaaag tgaccaagtt tatataatgg gacataaata tcctgatatg gatgctatgg
    36781 gagcagcagt tggtgtttat gatatatgta aatcctgtaa caaaactgca aacatagttt
    36841 tacaatctgt aaatgaatct atagaaatat ttataaataa aataaatgaa aataattact
    36901 ataaaaagtt gtttattgga aaagaggaag ctattgacaa ctgcactaaa aatacattag
    36961 ttgtagtagt tgatactcat agaccaaatt atactgaatg tgaagaactg ttaaagctat
    37021 cagagaaggt agtagttata gaccatcata gaagaggtgt agagtttata aatgatgcag
    37081 tgcttttatt ccatgagata tatgtatcct caacgtgtga aatggttaca gagttagttc
    37141 agtatatgga tgaagatgta acaataaata aacttacagc tgaagggctt ttagcaggta
    37201 taagtctaga tactaaaaac tttgcattca aaacaggtgt tagaactttt gaagctgctt
    37261 cttaccttag aaaagttgga gcagatacta tagaggtaaa gaaattcttc aattctgatg
    37321 taaaagattt cataataaaa gctgaaataa ttcaaagtac taaaataatt aataatagaa
    37381 tatgtttagc atattcaagt actgagatag atagtataaa tgttataata gctcaaactg
    37441 ctgatgagtt attaaatatc aaagaagtag aagcttcctt tgtattaggt gaaaaagatg
    37501 atactatatt tataagtgcg aggtctttag gacaaataaa tgtacatgta cttatggaaa
    37561 aattaggagg cgggggacac atagatatag ctggagcaca gttaaaaaat gtttctctaa
    37621 aagaagctta taagatggta aataaaataa tagaggagta tttggaggag gaagaataga
    37681 tgaaagttat attattaaaa gacgtaaaag gaactggtaa aaagggagaa atgaaagaag
    37741 taagtgatgg atatgcaaga aatttcttat ttcctaaaaa gatggctgtg caagcagata
    37801 gtgtagcaat aaaagaatta aatgaaaaga ataaatctaa agaaataaaa gcacaaaaag
    37861 agtatgaaga agcagtttta ttaggtaagc aaatggaaga aattaatata gaaatatatt
    37921 caaaagcagg agaaggtgga agattatttg gttcaataac ttctaaagaa attgctgagc
    37981 aattaaagaa acaaaaagac atagatgtag ataaaagaaa aatattatta gatgaaccaa
    38041 taagatcttt aggttcaact ttcgtagaaa taaaaataca tcaaaaagta actactaaaa
    38101 taagagtgga tgtaaaagaa aaacagtaat aactagaggt gattaagatg gaagatatga
    38161 cgagaattcc tcctcatagt gtagaatcag aacaatcaat attgggttct atacttctag
    38221 ataaagatgc tataataacg gttacagaaa ctataaagcc tgatgatttc tataaagagg
    38281 ctcacaaaat aatatatgaa tgtatgataa ctttaagtaa taaaggtgag cctatagact
    38341 tgataacatt aacagaagaa ctaagaaaac aagggcatct taatgatatt ggaggaatca
    38401 gttatattac aagtctttct actatagtac cgacgacttc aaatgtaaaa tactatgctg
    38461 atatagtaaa agaaaagtct gtacttagaa agcttataaa agcttctaat gaaataataa
    38521 atctaggata tagtggagca actaagattg aagatgtttt agaacaagct gaaaagagta
    38581 tatttgatat atcacaggag aaaactagtg atgattttaa atctataaac ttagttttga
    38641 tggatgccta tgatatgata gaaaaattat acactaacaa gagtgatgtc actggtataa
    38701 ctactggttt taaagattta aacaaaaaaa taaatggtct tcaaaggact gatttaatat
    38761 taattgcagc tagaccagca atgggtaaaa ctgccttttc tttaaacttg gttcaaaatg
    38821 cagccttaaa aggtgatgcc tctgtagctg tatttagttt agagatgtct aaggaacaat
    38881 tagtacaacg tatgttatct tcacaatcta gtgtagaatt aaaaaaaata aagactggaa
    38941 cacttaatga taatgattgg cctagaatta tagatgctat ggcagtatta tcagatgcca
    39001 aaatacacat agatgatact cctggtataa aaatatcaga actaaggtca aagtgtagaa
    39061 agttaaaaat agaaaaagga ctagatttag tgcttattga ttatcttcaa cttatggaag
    39121 gtgaaggtaa taatgaaagc agacaacaag agatatctaa gatatcaagg tcactaaaaa
    39181 tattagcaaa agaattaaac tgtcctgtag tagcactatc tcagttatca cgtgcaccag
    39241 aacaaagagc agaccataga cctatgttat ctgatttaag agagtctgga gcaatagaac
    39301 aggatgcaga tatagttatg ttcttataca gggacgaata ttatcatgct gattctgaga
    39361 gtaaaaatat aggagaagta ataatagcaa aaaatagaca cggtgaaact ggttctgtgg
    39421 aacttgtttg gcttggtgaa gttcaaagat ttggggataa gttgagagac ctataatcta
    39481 acgaatatcc acagaacgtc ctttctttct acaggaaaaa tgtggataca tgtcactaaa
    39541 tatgtccaaa cattgaaatt tagctaaaaa actatccaca ctatccacag atggcctgtt
    39601 gataagtgtg aataagtaga taaagcaagc ttttggcttg ctttatctgt gtataaatat
    39661 taattattag gaggaataat atggaaaaaa taagcttaaa atatatatat cccaatataa
    39721 ttaaagtact tgatgaaata aacttattta gagtaattga caataattta agagaatcta
    39781 ttgttgttta tgctaataat gtggataatc agtatcatat aaacatgact aatacaaact
    39841 ttggaaatat aattaacata tgtaaattag aaaaactttt agatgtggat aaatttatgg
    39901 aaaaagttat aaaatatgaa aaagaaataa tagagaaaga agagttttca aaaatagaag
    39961 aatatatgtt aaatattgga gaatactaaa aaagttgaaa aaaatacgaa tgtttgatag
    40021 aataagtaag taaagtgttc gagagtatat aaaatataga ggtgaattac atgaaaacag
    40081 ttgcaattgt tggttctcaa tggggagatg aaggtaaagg taaagttata gattatcttg
    40141 ctactcaagc agatgtagta gtaagagggc aaggtggaaa taatgcagga catacattag
    40201 ttgtagaagg taaaaaatac gcattacatc taattccatc tggagtttta aatccaaata
    40261 cagtgaatat aatcggaaat ggaatagtgt ttgaccctaa aggattttta gaagaattgg
    40321 aaatgtttaa aacggataat ataagtacag aaaatataaa aataagtgat agagcacatg
    40381 tcatattccc atatcataaa gaactagatg cattgtcaga agaagccaga ggcgatttaa
    40441 agataggtac aacaaaaaaa ggtattggac cttgttatat ggataaaaca gagagatctg
    40501 gtattagaat atgtgactta atggataaag ataaatttgc tataaaatta aaagctcaaa
    40561 tagatgctaa aaatgaaata gttaaaaata tatatggaaa agaagaatta tttgattttg
    40621 aaactatata taacgaatat ctaggatatg cagagcaaat aagaaaatat gtggcagata
    40681 cgtctgttat tgtatatgat gcagtaagag caggtaaaaa agtattgttt gaaggagcac
    40741 aagggacttt attagattta gatttaggaa cttatccatt tgttacatct tctcatccaa
    40801 catctggtgg atttgcaatt ggtgcaggta tagggccaaa tatgataaaa gacgttgttg
    40861 gtatagtaaa agcatatact acaagagttg gagaaggacc gtttgttaca gagcaaataa
    40921 atgaaactgg agataagata agagaacaag gacatgaatt tggggtaact actggtagac
    40981 caagaagatg tgggtggttt gatgcagtta tagttaagta tgcagctaga gttaatggat
    41041 tgactagtat ttcatttatg ttacttgatg tattaactgg atttgataag ataaaagttt
    41101 gtacgtctta taaaatgggc gataagataa taacggattt cccagcttca ttagatgatt
    41161 tagcaaaatg tgaacctgta tatgaagaat tagatggatg gaatgaagat ataactcaaa
    41221 tagataactt tgatgacctt ccagaaaatg caaagaaata tgtagcaaaa atagaagaac
    41281 tagttggagt aagtgtagat atggtttctg ttggtccaaa tagagctcaa actataataa
    41341 gaagaaatat atttgcttaa tattttatca attaaataat ttaaaatagt tgaagaagag
    41401 tatctcaaga taatgtttat agttattaga gatgctcttt tatatttgac ttacattgat
    41461 tttttattaa gttaaagcta cttatacata ttctaactga aattaaagtt gaatttatgt
    41521 agatacatat aggtttttta aaaagaattt tgttgaaaat aaatggctaa agatgatttt
    41581 aattatattc taaaaaaagt aaaaaaaact attgactaaa aattatatag acgctataat
    41641 ataacttgtg agtgaaagaa acgacgaaaa aagtcgaaaa aatgtggtct attagctcag
    41701 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
    41761 aaaatatggc tcgttggtca agaggttaag acaccgccct ttcacggcgg taacaggggt
    41821 tcaattcccc tacgagtcac cagtatggga ctatagctca gctgggagag cacctgcctt
    41881 acaagcaggg ggtcacaggt tcgagccctg ttagtcccac caaattttga attagagata
    41941 aatactagat tatatgctgg tgtggctcaa cggtagagca gctgacttgt aatcagcagg
    42001 ttgtaggttc gattcctatc accagctcca caaagtacat aaattcaaat taatataatt
    42061 ttctatgtta caggatactt ttaagtatct ttttttaatt tcatttttat tgtattaaaa
    42121 atgaaattaa acatacaaca aaattttgaa ttacttgcaa tgtgatagtt ttaaatataa
    42181 aatttagaat cattctagca taatattatg taatatagca aatttaattt atagtatcaa
    42241 taaatataga ctactaagaa tgtaaatgat ttgttagatt aaggtacatt agatatgtaa
    42301 atagaaaaat aaaaagtatg ttttaattta tgtgatataa gttttaaaca gagaagcaag
    42361 tttgctctta cctctctatt taaaaataaa aaaatgtttt ttaagggcta ttttatttaa
    42421 atctttccca tctcaaatca tctccagtaa attctaaaat cctaaactta tctaatattc
    42481 tagagagagt tctttgagtg taagtatttc ctatttgaga tggtgttaag ttggttgaaa
    42541 taatgatttt tttaccagca acaagtcttg tatttacaat attgaagatt tctccactag
    42601 taaaagaatt attaagttca gttccaaggt catctattat taataaatca caatcaaaca
    42661 agtttttata attatcttca cttatttgat tatttgtgtc tcttctaaat ttataatctt
    42721 caagaatatc taaaattcta aaagaagttt gatagattac aacattgcct ttatctaaca
    42781 attcctttgc aatacaattg cacatatagg tctttcctag tccagtagaa ccataaaaca
    42841 gtagattttc actattatcc ttcttaaaat catgaacaaa attttcgcag atacttaaat
    42901 tatttagcat attttctcta ggagatattt caccatcaga gcctttctta ggagaaaata
    42961 tattcaagtt aaaattcgaa aaattttcct gacttaaaat cctatctaaa ttagacattt
    43021 tataagcttc atttacaatt tcttgtttga ggcaagaaca tttttctcca ttaggtaaaa
    43081 atcctttatc tttacaagaa atacattgaa actttatttc caagtaatca agaggtatgt
    43141 tactttcagc taaaagtctt tctttcttta cttttaaaga ttctatattc tctttagtct
    43201 tctttacaat ttcatcttta gacttaggat ttaaaagtac tatcttagct aaactgagac
    43261 ctattttaga aatttcatca tctatagatt ttatctcagg gattctatta tatacctcat
    43321 tttttctatg ttctaataag agttcattat catctcttct cttagcatat ttagcaagta
    43381 tttttcttat tttatcctca ttcatttaat cacctactta aacttttctt tttgactttt
    43441 tttaataatc tcatcgagct catcagaggt atactgagtg aatgtttcgt tgaaattatg
    43501 aaacttagtt tttttgtaag tattgttgtt ttgagtagta ttaatctgct tcttattaat
    43561 attctcctta actatacgtt cttcctcttt ttgctttaaa tcatttaaat tttttatatt
    43621 tttttcattc caatttttaa ttataccatt tatataagat atacttgggt ttgaaatatt
    43681 cttagactta gaacatgcat ttattatcaa atctatatcc atattaaatt tatcaaacca
    43741 ggtgtccata agttcttttt ctgattttga tggttgtctt gaaaatccca attcattgaa
    43801 aatcgtttta tataaaatat atcgttcact tcttaccaaa aagctttctt caacatcttt
    43861 tggcgtatat aaatttgaat cataccaatt tctaataatg ccttcaatgt atttaactgg
    43921 ctttgatgta ccagttttat ctttgacgta ttcatatgcg caaagtatca tatcggggct
    43981 catattgtat ttattcatta tatccataat actcatcttt tcacttgggt cgagatatcg
    44041 cccaactatt ttatttatag aattaaacat ttttctaata cttggatttt cagaagtaga
    44101 tacaacttta tctgtattag attttataga tgaattattg cttaatatat tctcaatata
    44161 gaaatttttt aaatctaaaa attctattga ataattaaaa ttatcatatt cttcattgtc
    44221 atgtattttt ataatcttct tttcttccca gaatttccaa gcagataaca catcagacaa
    44281 tggtatattt aggtttttgg ctattgaatt attgtcgaac ttaggattag aagtaatatc
    44341 acatgcttgt ctatatccta aaagataaac ttttacgtat aacccgtccg ccattggcat
    44401 aaatatatct ataaagatat tgggaatagt gatttcacct aaatctattt cattgctttc
    44461 tttaaaaaac attttatcac ctcagattaa attatatcat tttttatatt atatttgtct
    44521 gtaaacaaat aaaaggtcac aagattagca tgtaaatatt ttctaaaagt attcattttg
    44581 aaaaggagga gatattttgg ttataaaatt caaccaaaaa gtaagaagaa cattatcagt
    44641 agcgattcta tttatggtat taataatagg ttgctttata gaaaaggatg acagtttaga
    44701 aacatacaaa gaaaaaacta attcaaaaat aaatcagtat aatatggatg ttatatttga
    44761 tgatgagact aagaggttaa tgtgcaatca gaatgttgac tacataaata atacaaaatc
    44821 aaacatagat aaaatatatt tccatatata tccgaatgct ttttccaaga aagattttgc
    44881 tccttttgaa aaaagtgaaa tgacaagggc ttatcctaat ggatttaacg aaggatatat
    44941 agatataaag aatatattaa ataataatag taaaatggag tataaaatta aaggagacaa
    45001 aaatgacata ttagaaatag aattagggaa agagttaaaa ccaaatgaaa gaatgtcatt
    45061 agatataaaa tataacgtaa aaataccaaa ttcaattgga agatttggat atggagaaaa
    45121 tactataaat gttactaatt ggtttcctat agcttgtgta aatgatgaaa ggggatggaa
    45181 tttaaagagt tatgaaacta ttggcgaccc attttatagt gagactagcg attttcatgt
    45241 gaaactattg attccaaata aatataaaat tgctcataca ggaaaactta ttgatgataa
    45301 acatgataat aagaaaatgc tatatgaaat agaagctgaa aacgttagag attttgcatt
    45361 tatactaagt agtaaatttg atgtaaacaa agtagattca aagggagtag ctatcaatac
    45421 atataattta aataaaaagc tttctaaaaa agcaactgaa gtggcaaaag attcaataaa
    45481 tatatttagt gaattatttg gaaagtatcc ttacaaagaa ttttctgttg tagcaagtga
    45541 cttttttata ggggggatgg aataccctat gcttgttatg atagaccaaa gtttatataa
    45601 tgaaaaaaat gaatttttac tagaatatgt tattgcccat gagacagcac atcaatggtg
    45661 gtattctgct gttggaaatg atgaaataag tgaaccctgg ttagatgaag cacttactga
    45721 atattctaca atagtttatt ttgaacaaaa gtatggtaaa gatatggcaa acaagcttat
    45781 aaaaactatg gagatacaga caaaaagtta tttgagtgaa aatatattta agccagcaaa
    45841 tgagtataaa aattctactg aatacagctt gaatgtttat acaaaaggcg caatagcatt
    45901 taatgaaata agaaaagaag ttggagataa agtattcttt gagactttaa atgagtacta
    45961 taataaatat aagcataaaa atgctaatgg aagagctttt gtggaactat ggaatagtaa
    46021 aggtgtagat ataaataaga ttataagtga atgtaagtaa atagtgaatg taagtaaatt
    46081 ttgtgcagag tattaaattt tatttaatac tctgtttttt tggtataata taatttataa
    46141 ggcagaaaaa gaggtgaagg aatgctaaag tattgttcag tagggagtgg tagtagtggt
    46201 aattgccact atatagggta taaaaatact aatatactag tagatgcagg attaagtggc
    46261 aagagaataa ctacaggtct taaagacatt ggtgtagatg cagataaatt aaaaggaata
    46321 tttataacac atgagcatgt tgaccatata aaaggagcag gtatattatc aagaaagttt
    46381 gatataccag tatttgctaa tataaaaaca tggtgttcta tgaaggacaa attaggtgac
    46441 atcaaagata aaaatatgaa agtatttgaa aatgataaaa cctactcatt aggtgatatc
    46501 atagtaagac ctttttcaat agaacatgat gcgtctgacc cagttggata taatttttat
    46561 acagagagtg atgaaaaaat gtctatagct actgatatag gatgtataac tcaaaatata
    46621 aaaaaacatc tatataaatc taaattagta gtattggaat caaattatga ccctaatatg
    46681 cttatgatgg gttcatatac atatgcttta aagaaaagag taatgtcaag tactggtcat
    46741 ttatccaatg aagatgcagc taatttctgc gttgaactta ttaatgaagg tacagaatct
    46801 atattattag cacatttaag caaagaaaat aattttcctg agttagcgta tgagacttca
    46861 aaaggagtat tagcatcaaa tgatatagta gttggacaag atgtaaaact agatgtacta
    46921 tcacgaaatg atgtttctaa tgtttatgag atgaagtaga ggtgaataac tttgctaaag
    46981 agaaattttg aaaatgttga ggataataaa attttatact ctataaagca gggtatgatt
    47041 ttagcgatac ctgctattat gactggttca actgctcttg taattttgaa tcttccaata
    47101 aaggcttatc aagaatattt atcaggcttg tttaatggag aggttacaaa tattcttaac
    47161 tttataaatg attctacttt aggaattatt tcattaataa tattattaac aataagctat
    47221 agttatggaa aaatatatgg gagcaagtat acagtacttg ttcctatagt tgcaatgtgc
    47281 tcttttttag tattttcaca tggtaatgag cttaattctt atatagaaat atttaaaaca
    47341 aagtggttat ttacatctat aattgtttct atgacatctt ctgtattgtt tgtaaaactt
    47401 acagaatctt ttgttacaag tgtaaagttt catacagaag gggctgatgc agactttaat
    47461 atggtagttt cggctatagt tcctttcata atagtagtat ttttgttttc tatttttaga
    47521 gtggtaatga taagtctaat tggcagttca aactttcaag atatatttta taatttattt
    47581 tcaaatgttt ttaataaaat gggaacaaac cttatgagtg ctctgttgtt tattttttta
    47641 atgcacttta tgtggttttt tgggattcat ggtagtaatg tattagatac cgtcgcaaaa
    47701 aatttatttg aaaatagtat ggcaattaac ataaatttag ttaataataa tcaattacca
    47761 acagaaattt ttactaagac cttttttgat acaatggttt tattgggagg atgtggctct
    47821 ttactatgtt tagtaattgc aatttttctt agtgaaaaac gtataaatgt aagaaagtta
    47881 gctaaaattg cttcaatacc agctcttttt aatatcaatg agatgctagt tttcgggata
    47941 ccaattgttt ttaactatat tatgtttgta ccttttgtaa ttactcctat aatcttaacc
    48001 gttacaagtt atgtggctat gtctacaggt attgtaccat gtacagtttc agctgtagaa
    48061 tggacctcac ctatattttt aagtggatat atgtctacag gttcaataag aggtagtttg
    48121 ttgcaagtat ttaatttatg tgttggtgtt atgatttata ttccgtttat aaaaatgtcg
    48181 caaaatagat atacatatat gtttaaaaat aatatagaaa atttaacaaa cttattaaaa
    48241 aggtatgaat taacaggtga acagcctaat cttctaaacc ataatggtaa aataggttca
    48301 atttcaaaaa tgttggcatc agacttgaag tttgctatga aaagaggaga aatagagctt
    48361 ttttatcaac cacaagtcaa ttataatgga aatgttgtgg gagcagaggg attacttaga
    48421 tggaaacatt caataggagg gttcatttat ccaccactag ttataatttt ggctaaagaa
    48481 gaagggttct tagatgagct tggagagtat attattaata aagcatgtat tgatttaaag
    48541 aagagtgaaa agctattgag aaatcctgtt aagttctctg taaatatatc tccagaccaa
    48601 cttgatgacc ctaagttacc agagcaaata aaaaatatca ttagtagtaa tgatataaat
    48661 cctaatatgt taggtataga aataacagag caaattgcat tatcaggttc acaaataatt
    48721 attgaacgta tcaatgcgat acataacttg ggaataaagt taattatgga tgattttggg
    48781 atggggcata gctctcttat atatcttcaa aataataact ttgatatagt taaattggat
    48841 ggttcattag ttaaagagat acttacaaat aaaagaagta gtgaaattat aatgactatt
    48901 gtaaatcttt caaaaaattt agattttgat atagttgttg agtatgttga aaatatagac
    48961 caaagggata agttatatgg tttaggatgt gaaatatatc aaggatatta ttatagcaaa
    49021 gcaatacctt ttgaagagtt tgtagaatat gcaaatcaag aaagagagat ataattttat
    49081 aagagcttgt aaattctagt aatgcataga tttgtaataa aattaagttt aggagaaata
    49141 tatgaatata agtatagtag tagtaggtaa aattaaagaa aaatatttaa aattaggtat
    49201 tgatgagttt aagaaaaggc tttctaagta ttgtaagtta gaaattatag agttggatga
    49261 tgaaaaagct ccagagaatt tgagtgttaa ggaaatggaa attataaaag acaaagaagg
    49321 aaagaaaatt ttagggaaga taaagcataa tagttatgtt atagccttgg ctattgatgg
    49381 taagggttta tcctctgaag agcttgctaa gactatgagt gatttagcag ttagaggtaa
    49441 tagtagtctc tgttttataa taggaggttc tttagggctt tctgatgagg ttttaggaag
    49501 ggctgattat aggttatcct tttctaagat gacttttcct catcagatga tgagattgat
    49561 attgttagaa cagatttata gggcttatag gataaataat ggagaaccgt atcataaata
    49621 aatgatacgg tttttgttaa tttgacactt aataatatta aaataacaaa aaagtattga
    49681 aagtatattg catttaaggt agaattaaat aaaatattat aacattataa tgtttatatg
    49741 gttgttaata aaaatttgga ggaaaatata tgataaaaat aatgttggcc tgttcagcag
    49801 gaatgtctac aagtttatta gttactaaaa tggaaagtgc agctaaagaa aatggtatag
    49861 agtcgcaaat ttgggcaata ccagaatcaa ctatacaaaa tgaaatagaa aaatgtgatg
    49921 tattattact aggaccacaa gttagatatg tattacctaa ggctcaagaa attgctaagc
    49981 catataatat accagtagaa gttataaata tgatgcacta tggaacagtt aatggagaag
    50041 ctgttttaaa tagagcgata gaacttaaca acactaagta atataaatta taatgtattt
    50101 ttaatatata aattgggaga atcaaaggat ggatgaaaag actatagaaa tatcttttaa
    50161 tataataggt tatgcaggtg aaggtaaagg attagctttt gaagcaataa aagaggctaa
    50221 gaatggaaat atagaaaaag caaaagaatt attaaaagag tctagggaag ttataaataa
    50281 agcacataga tatcaaacag aattaataca aaacgaagct agtggaaaca ggactgaaat
    50341 aagtgtcata cttatacatg cacaggacca cttaatgaat ggcatgaatt ttcaacagtt
    50401 agctgaggaa atcgttgatt tacatttgaa attacaagat aaataaaaat taaagataat
    50461 aaattaagag agttcttaaa agatacatta tgtgtagcac actaattgtt agattattga
    50521 ttcaatattt agtgagtaat acattaggat tattttgagg actcttttta tatttagttt
    50581 aaagttaata aatatttatt attaataata taagattata tgttataata ttataacata
    50641 taatttaaga ggtgaatcat gttaagaaat caaccattat atatacaagt tgtagatata
    50701 ttaaagcaaa aaatattaaa taacgaatat aaaattgatg aaaatattcc aacagaaagg
    50761 gagttagagc aagagtttgg agtcagtaaa attactatta gaaaagctat tgagatatta
    50821 gaatcagaag gatatgtaga gaaaaaaagt ggaaaaggga caaaagtaat aagtaatagc
    50881 ctttttaata agctatcaaa agcagagtct ttttcaagta tacttttatc taaaggtcat
    50941 aagttatcta aagagactat ttcaattgaa aaaataataa atgatacaaa ttcacaagta
    51001 tttagtatat ttggagaaaa atgtagcaag attactagaa tgtattattt agatagtaag
    51061 ccatatatat tttttactca ttatattcct tttgatgaaa atctaactgt tgaaagtttt
    51121 aataatgaaa gttctttata tatgtactta tataggagaa atataaatat aactagattt
    51181 gaagatgaat tttttataga agaatcagaa ctagaggtat caaaggcttt aaatctagct
    51241 aaagacatat tattaggaag aaaaagaaag acctatgatg agaatgataa tatagttgag
    51301 ctatcatttg caagatataa tacagaagta caaaactatg taattaaata cgaaatataa
    51361 taagaatcct gagtttattg aaatgataat atgctcagga tttttgttta gttgtaaaaa
    51421 taaaaaatat tataacatta taatgtttac aaccaaacat tataatgtta taatattttt
    51481 aataaacaag tgatattatc aattacataa aaattagttt atgttatgaa atcgttattg
    51541 cattataaat aaaggataat atcaataata cagggggata actatggaaa aattcgcaag
    51601 ttttttagag cgcaagatta tgcctgtagc agcgaagata agtaatcaga gacatatgag
    51661 agctgtaaga gcaggtataa tagcaacatt accactaaca atagtggggt catttttcac
    51721 aatattatta aacataccta ttgatgctta tatggatttt atagctccat ttaaagcaat
    51781 attagatata ccatttagat atactgtagg atttttatct ctatatgcta cttttgggat
    51841 agcatcatca cttgctaaaa gctacgattt agatacaaca ggtgttggta tgcttgcagt
    51901 tatggcattt ttagtttcta caatagttcc aactcaagta gttgaaccag taggtggagt
    51961 tatagaagct ggtagatgga tggatatggc aaaacttaat tcgccatcat tatttggagc
    52021 tatcgtaaca tcattaatta cagttgaaat atatcgtttt atgaaggaaa agaatattac
    52081 aattaagatg ccagcaggag ttccagtaga ggtttcaaat tcatttactg cattattacc
    52141 gacagcagta attttaatgt tattctgggt aataagacat attctaggat ttgatataag
    52201 tgttgcacta agcgctatgt tatcaccttt aaaaggattc ttatcaggaa atagtttagg
    52261 tggtgggtta ctgacagtat tcctaataac tttattctgg gtacttggaa tacatggtcc
    52321 agctataatg ggaccagtaa taagacctat atgggacatg tctatagcag aaaatataac
    52381 tgcattcaca gaaggagtat cagctcatgc aatgccaaac atatttacag aacaattttt
    52441 acagtggtat gtatggatag ggggagcagg ggctactctt gcattagttg taatgtttat
    52501 gttttctaag tcacaatatc taaaaagttt aggtagatta ggatttttac cagggttatt
    52561 caacataaat gaacctataa tatttggagc accaatagtt atgaatccaa tactagggat
    52621 accatttata attggaccta taataatgac aattctatca tatgttctta ctattacagg
    52681 ggttatacct atgatggtag caagattacc atttgcaatg ccagcaccaa tagcagcagt
    52741 aatgagtact aactggagta tactagcagg tatcttagta ttaatcaact ttgtgatatc
    52801 atttgctgtt tattatccat tcttcaaagt ttttgaaaaa caacaattac aaagagaaca
    52861 agaagaaatg agtgaaaatt tataaaaata catagatata gaggttttgt actatataaa
    52921 atatagaaaa agcttataag ttattaaggg tgaaggtagg aggaatgacc tttgccttta
    52981 attttatata ttttaaataa tgtaatttga ggaggagaaa gatggaaaga agattaggaa
    53041 tatcagtata tccagagcat agtaacatgg aagatgataa ggcatatata aaaatggctt
    53101 caaaatacgg atttaaaaga atatttatgt gcatgctttc aattacaaga cctaaggaag
    53161 aagttaaaaa acattttaaa gagattataa cctatgcaag agaattagga tttgaagtaa
    53221 tccttgatat agctccaaac atatttgata tattacaaat atcttatgat gatttaacat
    53281 tttttagtga gttaaatgct agtggaataa ggcttgacta tggatatgat ggaagtaaag
    53341 aagctatgat ttctttcaat ccatatgatt taaaagtgga actaaatatg agtaatgatg
    53401 tagcatactt ggataatata attacttaca agccaaatgt aaataatata tatggttgtc
    53461 acaattttta tcctcaaagg ggtacaggct tgccatatga tttttttgta agatgttcaa
    53521 atagatttaa aaagtatgga ataagaactg ctgcttttgt taattcaaaa tacgcaacaa
    53581 tagggccttg gaatataaat gatgggctgt gtacgcttga aatgcataga aacttagata
    53641 ttgctacagc aaccaagcac ttattcgcaa caggattgat tgatgatgta attgtaggga
    53701 atgcatatgc aagtgaagat gaattaaaaa gaatgtctga agtaaataaa tatcaagttg
    53761 tatttaatgt agtaactgaa gaaaaaactt ctgaagtaga aaagacaata atggcaaatg
    53821 agcaacactt tagaagaggg gatataacca accaagtagc aaggtctaca gaagtaagaa
    53881 aaaaatatgc taaagaaaat ttccctaagc atgataatac gaccatcttt aaaagaggag
    53941 atgtagtgat tggtaatgat gactttggaa agtataaagg ggaattacag ttgatacttc
    54001 aagattgtgc agatgaaaga aaaaataagg tgggtagaat aaaggaagac gaaataatac
    54061 ttttagactt tattgaacct tggagtaaat ttaaacttat tttagaggga gagtaaagtg
    54121 aagatagaca tattaataaa atgtggaaaa acagtagata aaagtataat tgatatagct
    54181 atattagacg ataaaataat agaagtaaaa aatcagatag atgattctaa gtatcaggca
    54241 aatcaaatta taaacctaga tggagaaaaa tttattagtg ctggttggat agatatacat
    54301 acgcactgct acgaaagact agacctttat aaggattatc cagatgaggt tggaataaaa
    54361 agcggtgtta ctactgtagt agatgcagga acaacaggag ctttagatat aggtaaattt
    54421 tatgaagata ctaaaaaata taaaacaaat gtatatgctt taataaatat tgctaagcag
    54481 ggaataacat ctcaagatga actttcaagt atgatgaata ttgatgaata tgaacttaga
    54541 agagcagtta aaaaatacaa agattttgtt gttggaatca aagctagaat gagtaaaagt
    54601 gttgtaattt caaatgatgt tgaaccttta aaagtagcta aaagaataaa aaatgaactt
    54661 aatctaccta tgatggttca ctttgggagt tctcctccaa cgatagagga tatatttgac
    54721 tatatggaaa aaggagatat attaacccat atatataatg gaaagccaaa tggaatactt
    54781 agaggcaatg aggttaaaaa agaaatcata gaagctagag aaagaggtat aatccttgat
    54841 gtaggtcatg gaaccgaaag ttttagtatg gatattgcaa tgaaatctaa ggatgcagga
    54901 atttttccag acactataag tacagatata tatattaaaa atagaataaa tggtccagtg
    54961 tataatctat ctacaacaat ggaaaagttt atatatatgg gctattcact tgaagatata
    55021 atagataaag ttactaaaaa tgcagcagat gcaataagtt taaaaaataa aggtttaata
    55081 aaagaaggat atgatgctga tttaactata tttgatgtgg ttaatgaaga aaaagaactt
    55141 caagattctt tgaataagtc agtaatcaca agtacgtcta taaagccaag agcagtagta
    55201 gtcaatggag aatatttaaa tataggtaag tctataggag agaatgagta gaagggagaa
    55261 actattgtat gagtagtata tttgaaaaat actctgtaag acgtgttata aatgcttcag
    55321 gcaaaatgac tatactagga gtatcaacag tagataagga agttattgaa gcaataggtg
    55381 aagggtgtaa aaatttcttt ataatagaag aacttataaa taaaacaggt gaatatatag
    55441 caaatttatt agggtgtgaa agtgcactaa tagtttctag tgcatctgct gggattgcac
    55501 agagtgtggg agcaatgata gcaaaagatg atatgagtct agtttacaat gtaaataatc
    55561 ctaagaaaca agttaaaagg gaaataataa ttccaaaggg acataatgtt gattatggag
    55621 ttccagtaga agtaatgata agacttggag gaggagaagt tgttgaggcc ggttatgcta
    55681 atatgtgtaa caaggaacat ataataagag agataaatga aaatacagca gcaattatgt
    55741 atattaaatc tcatcattgt gttcaaaaga gtatgctaag tgtattagat gcagtacaag
    55801 ttgctaaaga atataattta cctttgatag tagatgcagc agcagaagaa gatttaaatg
    55861 tatattatga attgggagca gacctagtga tatacagtgg tgctaaggct atagagggtc
    55921 ctagctcagg gttagtaata ggtaagaaaa aatacataga taatgtaaag cttcaatcaa
    55981 agggtatagg aagagctatg aaaataggaa aagaaaatat tgtaggtcta acatgtgcaa
    56041 ttgagagata tataagtaat gataaagtaa ctttaaaaga aatggaggat aagctaaatc
    56101 catttataga caagataaat actataaagg gagtgagtgc tagtataact agggattcag
    56161 caggtagaga aatacttcgt ggagaaattg actttaatga agatataata aataagtcaa
    56221 cacaacaaat tataaatgaa cttagaagtg gagagatagc tatatacact agagactata
    56281 aggctaatga aggcaagatt gagattgata taagaagtgt aactggaagt gatttagata
    56341 ctatttataa taggataaaa aatatagtag aaagttagag tgtagagtaa gtgacattat
    56401 aatgatttaa taaattaaat tttaccaatg gaggggataa atatgaattg tataccacaa
    56461 tattataaaa atagagtttg tttaaatgtt cttgcaggaa gcataaaaaa tgctaaagaa
    56521 gtatatgatg cttgtgacgg gcatgtattg ataggaatac tatcaaagaa ttatagcact
    56581 gtagaagcag ctattgaaga tatgaaaaaa tactcaaagg aaatagataa tgcaatatct
    56641 gtggggcttg gagctggaga cccaaatcag tcaaatatgg tatctcagat ttctaaagaa
    56701 cttcagccac aacatataaa tcaagtgttt acaggtgtgg ctacgtctag agcattgctt
    56761 ggtcaaaatg atagtataat aaatggatta gtttctccaa ctggtaaacc aggatttgta
    56821 aaaatatcca caggtccatt aagttcaaaa gaaaaagatg gtatagttcc tatagaaaca
    56881 gcaatagcta tgatgaaaga tatgggagga agctcaatta agttttttcc aatgggaggg
    56941 cttaaaacaa aggatgaata tatagaagtt gcaaaagctt gtgcaaagca taatttttat
    57001 ttagaaccaa cagggggtat agacttagat aacttcaaag agatagttca aattgcactt
    57061 gatgctggtg tagaaaaagt tatacctcat gtttatacat caataattga taaagaaaca
    57121 ggtgaaacga aagtggaaga tataggagta ctttataaaa taatgaaaga attattggga
    57181 tagatttatg aatatattag cttttggtga aataatgatg agactttcag tacctgacta
    57241 caagttctta acacaaacca atgaactaaa ttatataact acaggcacgg gattaaatat
    57301 attatctgga cttaaaaact ttggatacaa tacctatatg ttgacaaagt tgcctaataa
    57361 taatgtaggt aaagcatcaa gtgcaaatat aagaaagctt ggagtaaaag atgactttat
    57421 cacgtatgga gggaatcata taggagtgta ttttcttgaa aatgggtatg gagaaagacc
    57481 ttctgaagtg acttatttga ataggttaaa tagttcattt tgtgagagta aattacaaga
    57541 ttatgatatt gataaatgtt tagaagaaat ggatatagtc catatttgtg gtattgctat
    57601 gcaacttaca gattcagtta aagagattgc tttagaacta gctagaaaat cttatgaaaa
    57661 ggggataaaa gtattctttg attttaattt tagacaatca ttaaatccag aaaggactta
    57721 tgatgattta gtaaatgatt ataagaaaat actaccatat tgtcatgggc tgtttgctag
    57781 ttatagagat ataaataaga tacttaatat agatagtcta aatataaaat ctagttcaga
    57841 attgattaaa aaagagtatg atatagatat tgtagctgga actatgagaa gatttgatga
    57901 tatgaataat agatttataa agggatatat atcaaatgat gatggttatt ttgaaagtag
    57961 ggagtataag ctagaaatat atgacagggt tggtgccgga gatggatatg tagcaggaat
    58021 tatatattca tatttggaaa atatgaagag tgaagatata gttgagtttg gggttgctag
    58081 ttcggtgtta gctcatacaa cttatgggga taattcttta gtaagcacag gtcatgtact
    58141 tagactgatg aataatgaaa aaatagatat attgagataa tacatttgta tataataaaa
    58201 tttttatctt ttttagacac atttttgact tgcttatatt tgttaaacag agttattttt
    58261 aatgattata aatattaaat taaaagttaa ataataaaat agtagaaaat agcattaact
    58321 atagaaaaaa tggttaatgc tatttttatc gttaatattg attaatttta tattatagaa
    58381 atgaactttt ctaatgctgg atttgtattt tttttattcc atacaactac aacttcaatt
    58441 ctattttcta cttcttttat aggtactatt gcaatatcac gttctatcat tgtatctgta
    58501 acacaaaatg aaggtaaaat ggatattccc atgttagaag ataccaatga ttcaatagta
    58561 ttaagattat ttgaacgttg taccacatta ggtacaaacc ctgcttcacg atatctattc
    58621 tgtatcattg acttttgtcc atactcatcc cctgtttcct ttacaaaaat aaatttatca
    58681 tgttgtaatt caattaaatc gatagaatcc tcattgtaaa gtggatgacc aggaggtaaa
    58741 agagctacca aagaatatct ttctacagtt ttatattcaa aatcttttaa tggttgattt
    58801 tctggattaa taacaaaagc gaggtccata gaattatttt ttactaagtt taaaagttcg
    58861 gttatttcat tagtacaaat atccatagat atattagggt atgtattaga aaattctttt
    58921 aaatattgtt gaagtttgtt tttttcatat cctattaagg ttccaatgct caaagttcca
    58981 aaaaaaccat gtgcaaattg ttgtgttttt gcaatagcat cattgatatt ttttattata
    59041 gtacgtgcct cagataaaaa aacttttcca gctggtgtta gttcaacatg acgtttactt
    59101 cttgtaaata gagttacatt tatttgttct tctaaagctt ttatttgctg tgtaacagca
    59161 gtttgagaaa tataaaattt ttctgctgtt ttggtaaaac ttagattttc tgctacagat
    59221 ataaaatatt ctaattgttt agtattcaaa taatcacccc tatttaaact tgattgtttg
    59281 taaaccttat ttatctataa ttaaagctta ttatagtata agaaaattta cttttaaaga
    59341 atattagaaa atatttgtat aaaaatatat gttttaagta tatctttaca ttagatttta
    59401 taaatttatt aataagaata acttatatat tccaatgaaa acgatattgg atttaagtaa
    59461 gtttgtctca taaaatgaaa gtatatcaat tgataatact ttaaataaga agggagaaaa
    59521 aatggaagat aaattttatg caaaaggcaa cggaaataac ggatatatta aaaatcttga
    59581 agtttgttcc tttaataact tagatggaac ttgtggaatg tttcaaatgg ctctgtacaa
    59641 aagagatgaa aaatactatt tatatggatg ctgttttgga ggaaataaaa aaaatggagt
    59701 aatgattagc gatattacag acccttataa tccacaattt ataaaacatt ttcaaatgtt
    59761 agaccctaaa gagtatccta caacaacaac tcccaaaatt caaatagcag atgatttaat
    59821 gatagtagca atgagttgtg gaagtggacc aggagcactt gttgaccaag ctaaattagc
    59881 aaatattaag tgtgaagcag gaattagaat atatagttta aaagaagacc ctttaaatcc
    59941 taagttttta ggatattggg attgtggctt aaagcatgta atgggtgttc atagattcat
    60001 gtacaatggt ggaagatatg tacatttatc aagtgattgt gttggctttg aaggtctgat
    60061 ttatagggtc atagatataa taaatcctac taatccagtg gaaataggta aatggtggag
    60121 accagaccaa tatgcaaatg gatatccaaa tagaactttt gatgcaggag cacctcattg
    60181 cccagaattt atggataaag gatggcttca tggacctcca tttgtaagag acggaaaagc
    60241 atattgtggt tatggaggag ctggtttagt tgtattagat gttgaagatt taacaagacc
    60301 aagatgctta ggtgaattgc catttatgcc tgcattttct agtagacttg caggtgcaag
    60361 aactcataca gcattaccat tgccaggaag agatttagtc gttgttcaaa atgagggaga
    60421 aagattccag ttctttaaac cagataacat tacagatgtt caagctatga ataatataca
    60481 tatggttgat gttagtgacc caacaaaacc aacattaatt gctcaatttc catatcctga
    60541 agttccaaaa gatttccctt atcctaactt taatgttgcg ggattaggaa aaccagggcc
    60601 atttggccca cataatcttc atgaaccaat ggataataag ccatggttag agcaaagagg
    60661 agatagagta tattgctgtt atttccatgc agggctaagg gtttatgatg tatcagaccc
    60721 atattatatc aaagagctag catattttat accaccaaat ccaaataaaa caccagaaga
    60781 atcttatttc ccaggattcc caggaccacg cttggcagta acagaagatc ttatcgttga
    60841 tgatagaggc tacatcatca tagatgcttt agatgatgga ttctatatat taaaaatgaa
    60901 agaagattaa ataaatacta aagagggaga gttgaaaatg aaaatactag gtatttcttt
    60961 tggaacaaaa aatggaaata atgacacaat gtgtaaagtt gcacttaaag gtgccaaaga
    61021 agcaggggct gaagtagaat ttattcaaat gtcatctatt aacattaaac attgtactgg
    61081 atgttgtgcg tgtgttaaaa ctttattatc tgggaaaggt agtatgtgtg tattaaaaga
    61141 tgattttgaa tggttgcttg acaaaatgaa agatgcagat ggtatagtag tttcagaccc
    61201 tatttttgaa gaaggggcat caggattatt ccatactata atggaccgtt ttggacccag
    61261 agcagataga ggaaataaca ttataggaac aaaagttgca gaagctatag gaggaaaaat
    61321 tccagattca agaatgctta atgataaagt aatatcattt atgggaatag gaggttctga
    61381 ctggggtaca agagttcaat gtgaacatgc tatgctagct ctcataccaa tgtggaaagt
    61441 tatagataat gcttggttcc catgggcaaa ggaattagta atggatgata caaggcttgt
    61501 tcaagttcat caaattggtt taaatatagt tgaagcagca aaagattttg aaaaagcttc
    61561 atatcaagga gaagaaggtg tatgtccaca ttgccacaat agattattct accttgagcc
    61621 aggtactaaa aaggcaattt gtgctctatg tggtatagtt ggagagttag atactataag
    61681 tggaaaaact atatttagtt ttccagaaga gcaattaggt catgctcatg atactttacc
    61741 aggtaagttt atacatggtg atgatattaa gaaaatggaa ggtcattatg cagaagttcg
    61801 taaaacacaa gaatttaaag aaagaaaaac atcttataat tttatagagc cattaactaa
    61861 agaaaagcaa gctggagttt cattaggata ataattaaaa tactaagact cttttttaag
    61921 gtatgttaga tgttatagga ggcttattgt atgaatgaaa agtcaagttt taaacaaatt
    61981 gaaattattt tagcagttat ttttttggta cttacatatt acaaggttag attaactcca
    62041 gcatttattg ttatatttat accactatat ttaagttatt ttgcagtatc tatgattgta
    62101 aatcaaatag caattaagct attggagata aggcaagcta gggaagctac taattacaat
    62161 ataggaaatg gatagaaaga aaaaagaaat attaacactg tctatgggga ttgctttact
    62221 acctccttta tgggcagtat tagcacctta tataggaata aaaactggag ctgtagcact
    62281 aatctgtgca ggattatatg taactaatgg taataaacaa aaagatggat taaaaataat
    62341 gtttggtttt tggtgtggag atttatgggc agtgttagcc attttaatta tgggatatat
    62401 gaatttcaat caaaacctag aattattttt aacacttagt atattaggtt tttttgcagt
    62461 tgtaattgca tccttatttg aaaaaataat tttcctacca tcatggcttt gtggttgggc
    62521 tataggtcta actattatga caggtgaaaa tattattaat ttacaagata tttgtattca
    62581 aattgctgta gctatggcag taggagtatg gtacgtgggt gcaggtgtag atttgtttac
    62641 aatatatatt ttgagtaaag ataggaaaaa gggaaattga aagcatgtga atatactttg
    62701 attataggag aatggctatg ggttttgaat ttaaaataat gagaagttta atatatgtag
    62761 gacttgccaa ggaagaatat agacctaagc taatggactg gttatatcgt caccatattc
    62821 cagatagtat tagcactttt ggaccatatt gtactaaata tgccttttat caagcatatc
    62881 ctacaccaaa tgaaggtgag cgttttggtg cacgtaagat gcaactaaca gaacattatt
    62941 ggcttgtaga tgaacatatg cctgagatgg caaatagaat tatgacagaa tatatgccta
    63001 tggatgttct acgttggcaa gggtgtatac cagatgtaga aaataaaagg gttcatgaaa
    63061 atgcagaaag tggagatgca ggacgtgcag taggtggaga taatggatgt ccaccattta
    63121 tatttgcctt tgttccaata aactgggaag aagactttag aggaaaagga cgtactgtac
    63181 aagatggacc aaactatcgt tggcaattta tgattaagta tccagatggt atctctaaag
    63241 aagaaggaga aaaatggttc tatgatgagg tagtgccata ctttacaaac tgttgctatg
    63301 ttaatcgttt tgtcagtagt aaaataatga ttaattatgg agcaactgct tttgaccgtg
    63361 tatcagaact atggtttgaa ggggaagaag aatggtataa agctgtggtt gaagagacaa
    63421 agtcgtttat taaaaaacca gaatgggcac aagaagagga gttcccatat ttaaaaccac
    63481 aattcaatat cgcatcagta ttcttaggtg atatagcaac tatggatgca tactcacagt
    63541 atcgtggata tataccaatg agataaaaat tatagtttgt gataagtaga ggaggcattt
    63601 actcttactg gtagaagaag acttattgta tccttaaaag taggtgaata tatagtttta
    63661 tatttaaatt tatataaaac taagactaat aaatatgaaa caatagaatt agaataattt
    63721 gataatattt gttaaaagag catttagaat gtgtttaaag attaagatat tccttttatg
    63781 gtagcagata gttgaaataa taaaactgtt atcatgaaag gaatattttt atgtatagga
    63841 aatttttaaa aataaaagtt aaatatgttt aagaacatct taaagtgaaa gaatcattgt
    63901 tagaaatatg tagacaatat agtctttata acgtatcata ttaacaagtt tacacttgga
    63961 ttatgaaata aaaagagtaa ttccaaaggt tgtaaatact atgctgactt tgatacttca
    64021 aagtttaggt gattttgtaa ttgttaatag aaatacgttt aatgagcata aagaatttat
    64081 ctaataatag gagaatataa tgtatacaat tggacaagta tctaaatttt taggaatatc
    64141 tagagataca ctaaaatttt atgaggacaa aggattagta aatcctaaga aaaatgatga
    64201 aaatggatat agaatatata atcaggttga tatctatgat attgcaacaa taaactttta
    64261 tagagaaatt gatatagaaa ttaaaaagat tcaagaaata agaaaaagta agagtataaa
    64321 caatttagaa ctcttactag aagagaagga acaaatcata ctaaaagaga tagaatataa
    64381 aaagcttctc ttaaaaaagg ttcagttagt caaagaagat tgtgacaaaa taaaaacata
    64441 ttttggaaaa tacacaataa aagaaatgaa gcctttggaa gtaaaaggta aaataaaaca
    64501 tgttactaac tatgaggaat atgaagcctt aaaagagaat acaaacaact taaaaaaagc
    64561 agttacatta actgatttaa gaagagtaat aaattttaat gaagaaggta taatagaaga
    64621 aaaatttata attgtaaggg aagtagatga ctacaacaaa atcatagaag gtgaagttct
    64681 atctcatccc aattgtattt atactattat tgaagatggc agatggtcaa ctggaggaga
    64741 aaatattgat aataaagtgg aagacagatt aagagaaata gcaatagaaa atgaatgtga
    64801 attacttgga atagtctatg ttaatatatt gcttactact tatgaagaag ggattgagcg
    64861 tatattttta gagatttatg cacctataaa ataatataaa agcattgacc tgggacttac
    64921 tcataggttt aaactcatga tataagcagt tataacttat atagcaatta aatattaatt
    64981 tgaaatgagg attacttatg agaacagaga aatcattaaa aaggcctata gccagtttta
    65041 ttattctaac aaatataatt tttttaccac tttttttatt ggtagtagtg actataatgt
    65101 taaaacttcc aacagtgata tctgacattg cgttgtgtat atcagcgtgg tcatccactt
    65161 ttgcatttat gatattattt aaaaaaattt atccaggtaa gaaattttta gtacacgtaa
    65221 aagatagatt tagaaataaa cttaaatttt caacagtatc tataataatt agtatacaag
    65281 tcctaatagc tgtagtagtt atatttatat tagcatctaa aaatcatgga ataatgccta
    65341 actttacagt ttcatcatgg ggagcattta tatatttatt tttaaaaaat ttatttgcag
    65401 gatcattagg tgaggaatta ggttggagag gatttgttca aaatcatctt cagaaaagac
    65461 attctccttt aaaagcatcc ataatagtag gtttttggtg ggggatgtgg catttaccaa
    65521 tatggtttac aacaggattc aaaggacttg atttaataaa atatattata ttttttatga
    65581 ttgcaattat atcaatcagt attataatga caacttttta taatctaaat aaaactttga
    65641 taatacctat tgtaatacat caactattta attttttaat agggataatt aatggagatt
    65701 taatagagct tataaattac tatgcaatgt tatatttaat atttgcaatt gcattaatag
    65761 ttataaatcc aggaaatgta ttatataaaa atattagaga gtaatatata tctttatggt
    65821 aagatataaa gatattatta gaagaacatc caaggaggaa taaacatggt tttactgatt
    65881 gttgatgcac aaaagttaat aacaaacgag aggttatata aatttaatga atttgtagta
    65941 aatgtagaaa atttaattga tacagctaga aaaaataata tagaagtaat atatgtacgt
    66001 catgatgatg gagttgaaaa tgaattgact aaaggtaaaa atggctttga aatatacgaa
    66061 aaatttaaac catgtaatgg agaaaaaata tttgataaaa aagtaaatag tgcttttaaa
    66121 gaaactggtc tattagaata cctaacaaat aatggtgaaa aagatattat tattgctggt
    66181 ctccaaacag attattgtat tgatgccact ataaaatgtg gatttgaaca tggatttaat
    66241 ataatagttc cagcttattc aaatacaact gttgacaatt tttttatgtc agcagaacaa
    66301 tcatataaat actacaatga atttatatgg aatgaaagat atgcgaagtg tatttcatta
    66361 gaggaaacac ttagaaaaat gaaataaaaa tagagtatga attgtatatg tacataagtt
    66421 gatttcataa aaaaggatgt taaattccaa gttaaaaatt agattaaata tattatatta
    66481 tagataaggc acatcaattt tattttaaat taatgtgctt tttaattgta gtaatttatt
    66541 tataatcaat atgattttta ttatttaaat caatttttct tgagtttctt tgtctaaaga
    66601 aacttttttt atttataaaa tctacagtta tattatagta tctattttag agaagtttaa
    66661 tttttgtatc aatctaaaac aaaagttata cttaaatgaa attgaaaacg ataaaattct
    66721 caaataaaat aagtattgtg gaggtgatga aagaatccaa tgataatatg aagactaatc
    66781 ttaataagaa agtaggggtt aatggtagtg gacaaagaaa ttgtatcaat tctccagatt
    66841 ttattaaata gcacattgat tacaactaat ggattacaag aggagtctaa ttcatcaaag
    66901 agacaaatta cttatagaat aaataaaatc aatgacatgc ttaaagtaaa aaatgtacca
    66961 cttatatgtt taagagctga taaagatatt atcgttagaa aagaaacaaa aaaagcaatt
    67021 aaagaaattt tagaaaaaaa ctattctaag aatacttatt attttagtaa gacagaaaga
    67081 cttttatata tgtatttaat gctttttatt aacatggatg atatttcaat taatcatttt
    67141 ataaattcta taaaagtaag taggtctact gtaaatcttg atttcaagga tttgatacca
    67201 gagcttgaaa agaagaacat taaagttaag aacaatcgta taaatggata ttacatagtt
    67261 ggcaatgaga tggaaattag gagagtgctt attaaaaata ttatagaaac tttgtctaat
    67321 gagaaaagtt caagagtatt tgatattttt attaaagaat ataatttaga tagttttgaa
    67381 gaggctaaaa aaattatatt aaagctaata aaaaaatatg aaattacttt tgttgaagat
    67441 agattagtag aaattttata tctgattttt gattatccgc ataaacacta tactttcaaa
    67501 cattttgaag tatagaaaac tattcatttt accacgaatt taccacgatt gaatgagcct
    67561 tgatatgaca caaaaacaac atgggagata gcacaaatat ctgtgtatct cccgtttttc
    67621 attcatgtag tatgattatt ttactctgat ttcaaatttc aagattgctt tcatcatttc
    67681 tgtatggatt tgacctttta attcttcatc aacctgcata atgattttac cactttcaga
    67741 gtggtaaaac ggacgcaagc ataatttaca tatataagcg tcataaaact tcaaaatctc
    67801 tcttatggca gtttcatttc cagcagacgc ttgacatatt acttttaatg tagggtagcc
    67861 atattctttt ttcattggtt caattccttt ctttcaatac tgtttttagt ttcttcaatc
    67921 cgttacttct tctgcgataa atagcagaac gtgatacatg atataattcg gctatttctt
    67981 ggtcgctcat actttgaaaa tagcgtaata gaattacatc acgctcttta ttcgataact
    68041 tatataaagc aacagcaagt ttctcatttg atatttgaat tgtaaaattc aagacttcaa
    68101 acgaaatgta atcacaagaa tagttatcct tagttacaca ttcaacattt ttcgtatctg
    68161 ataattcaca gaataaaact tcacgcttag aacgtcttgc aaactgtctg tttctgtttt
    68221 ttactgtgca tttaataaca gtcatcatca aagcattaaa ctgtattcta acgatatgct
    68281 cgaaagaaga tgggttcata atctcacctc ctttccgcat acgctgaaat atataaacgg
    68341 agataccgtc tatatctttg gaaaatacaa aaagaagaac aagaaaaaag tccgtacaaa
    68401 atataccgtc tgtacgaact tttaatattg taaaatattt agttgttgat aaaatgctgt
    68461 taggtagtgc ataatcgtat aagattattc ctaaaacttt ccacagcagt tactttcaat
    68521 gcttttatat cttgcttttt tagtcctatc ataataacct ttcttatcat aattgcaaat
    68581 ttataaattc attagtatta aaactgaaat tcaaatacat tactaaaatc attgttcaag
    68641 acataataaa ctttattttt ttctgtagaa tatacaaatg aaaccctagt tgcccacaag
    68701 tctttttgag aaacagattt taatacttta aatgcgtcat ttactgaaaa atctttatca
    68761 tatcctgcca ataactcttt agccacttcg tatcttgtat ccccagaaac aatgtacggc
    68821 tttgttatat ctggttttag aatagaataa tttgtaataa gtgaaaaatt ttcttgttca
    68881 tatctatacc caattccggg ttcaataatt aaaactcgtc cttttttatc agaaagcata
    68941 gcttgcatag tagcatctgg tgcataaata atttttttct ttttaacaat gtccaaggct
    69001 ttatcaaaag aaatctctgc cttaatgaat ttttctgtta attcagatat tgtaatacaa
    69061 tcctcgcttt ctatatacct tgcattttca ttaccatgaa cataaagaag tgttcctaca
    69121 ttaccatttt tatttatccc atgaaatgaa tgatacatac cgtcaggcat tttaatacca
    69181 atataaaatc tattttcttc ggtaattaca gtatgatccc atacagaaag gtcaatatca
    69241 aaattaaaac caacaatttt atctatacca ttgtaaacaa atcttgtaca catataatca
    69301 ctcctcaaaa tactacttca aatagttatt taactaattc aattagtatt ttaaaatgtt
    69361 tttgtaggaa agtcaataaa taatcataat tttctaattg tgttattgac tacactaatt
    69421 caattagcta gaattgtgtt tgtatgatat tttgaatgag gtgcataact atgaaaacca
    69481 ctaaagaagc agtaataaaa gcaacatcag acttagcaga tgaaaaagga ttaaataacg
    69541 tttcgttaaa agctgttgct caaaaattaa acattcgtac accatcttta tataatcaca
    69601 tagaaagttt agataatctt cttttagaag ttgctcataa tggtatgcgt gacatgaatg
    69661 aaagaatgat gaaaattgcc gttggaaaaa tagggaaaga agcaattaag ttagtttcta
    69721 tcgaatacct aaactatatg attgaacacc ccggagttta tgaaacaatt caatgggctg
    69781 tgtggcacgg aacagaggaa acagcaacca tttttaacaa ctatctttca ttattaacta
    69841 ctcttatcca atcgtgcagt cttaacaaag acaaaacact tgaaatatta aatatgctta
    69901 ctggaataat tcacggttat acaactttgc aattaggaaa cgctttttct gcctcagata
    69961 aagttagatt tgaattagct gaagcgatag atactttatt agtcgggatt ttccagaagt
    70021 ataaataata tatttaggct tgcaaatcaa tacagaactg caagcctttt tcttttatcc
    70081 aacaatcttc cagttactat tccttatata gcccaaactt gtaccgtgac aatttcattg
    70141 atattatccg atttaccaca aatcaaattt tatcttttct tcctcttgat taccctccac
    70201 agaaaagaaa aagccctgtc aagagccttg ccaccattgg tggtgctttg cactctttac
    70261 tgggcttttt gtttgctgta tcttctacaa agaggaaaaa agttattcgt cctcatcatc
    70321 aaaatggtta tttttcaata cttcccgtaa taattccata tcttcttttg aattggggtt
    70381 tatcattttt acgacttgat aaggtgtcct atatttatgt ccctctatgc tttccccgaa
    70441 catatccatg ctgtataaat catcatagcg gtctaacagt ttttgaaact tcaaatgctg
    70501 gataaattct ttgattggat aaaggtcgga cgctacaata cttttcccgt tgatgtagtc
    70561 ggtaatatat tcccgtaact tttccaactc atattccagc cattcttcct cgctgtcaaa
    70621 gaagttgtca taatgtccat gttttagttc tgcatttaat cgttcttcaa ttcttaaaaa
    70681 ctggcagact tccttttcgg attgattaac atacttccat tctcccgata ataattcatt
    70741 gggcattaca cccagcacgt ccattatctt ttctaatgta tcaaaggtag gaaaattcac
    70801 acctcgttca atcttggaaa ggctctgcat attgataccg attttatccg caagttcctg
    70861 ttgtttcatt cctctgtgtt ttcttatggt ctgtatgttc tctcctaaga aactgatttt
    70921 cttttcataa tgctccataa cttagtataa ccgctccttt cgttgctttt cttggtattt
    70981 ataagcatat catacttaat ctacatctaa aagtcaacaa aaacttcttg acaagtaatt
    71041 ctaatggcgt tattatcgta tcagatagag tgattaagca cgatagggtc aatcacacgc
    71101 ttgagtaagc atagagaaga tacattttca ctttgatagc atgaataagc atggactatc
    71161 agaccgaaaa tagtggtttc caaaaggggc tggctggacg gggcacgtcc cccagacgtg
    71221 ttcggacaga cagttccttt tggaaaagga acgcctaaag gcgttcgcag agggcgtata
    71281 tgtaacaccg ccctgcgcat acatgtcata gtacctagaa actgtgataa ataaaggaaa
    71341 aaacacgatt tttaccccgc aaaaaaacgg gaagtacgaa aggagtaatg cactataact
    71401 acacacaaaa atttatccgt taagattgat tacatcagta ttgtattcga tactgcaacc
    71461 gccgaagatg tcatcatgca cattttaggt ttaccgactg atattttcaa tgtctatccg
    71521 gcaagcatta aattcaagac ttatcaagca cgctggcaga ttggagatat ttatgtatcg
    71581 ggggacgcaa gaaagacaga ggacaaccca caagggctag gctgttatct tgttatgact
    71641 ggcagaggtt gtgatgatat tttccgtatt cttgatagta ggaattatac ctttggagat
    71701 atgttcaaac attgtgagcg aagatatgga ctggataact tccatttcac tagactggat
    71761 attgccattg atgataagaa cgaaaagcca ttttttacca tagagcagat aaagaagaaa
    71821 tgcgaaaaag aggaatttat ctcgaatagt gagggctacc actttgacga aagcaagttt
    71881 gatgatttcg acaccgcaaa gaccgtttat atcggtgctg ggaaatcggg attgtcctac
    71941 cgcttttatg acaaggataa ggaagtctgt tcaaaacata ataagacact tgatgaagtc
    72001 ggcagttgga aacggacaga aatgcaactg cgtgatgata aggctcatgc ttttgccatg
    72061 acattcaagg acagaccgct ggaactggga gaactggctt tcgggctatt ggcaaacaac
    72121 ctacgctttg tcgtaccaaa cagaaacgaa agtaataaga gcaggtggaa aacttgtcgg
    72181 ttttgggaac gctttttagg ggctgtggaa gtcttaaaac tgcaagtacc aaaacagcaa
    72241 aattcccttg aagaaacaca gcaatggctc acagagggtg gcgtgatttc cgctgtcaaa
    72301 agtttttact tcttggaaga acatgacgca ttgggtggac tggaaaaagt gggaactatg
    72361 cttgataagg caagatacag taattccctt tccagtaaac taaccgcaca tttacagatg
    72421 ataaaccgca ccgaccttat cccttatatc caatatgaca cgaaacatgg gaaagggggt
    72481 atctgatgaa taacaacgat attcccgtat gggaaaaata cacccttacc attgaagaag
    72541 cgtcaaaata tttccgtatc ggagaaaaca agttaagacg attggcagag gaaaacaagg
    72601 acgctggctg gctcattatg aatggcaacc gcatacagat taaacgccga cagtttgaac
    72661 aggttattga taaattgaac gcaatctaat gcaaatgagc cttgtatgtg ttatgatgaa
    72721 cacaagtcat atcaaggctc tttccaacaa ggaaaggagc agacaccatg aaagaaaaaa
    72781 gacgggatag caaaggacgt atcctgcata ctggagagag ccaacgaaca gacggaaaat
    72841 acttatataa atatgtggac gcatttggaa acacaaaata tgtgtatgct tggagattga
    72901 cacccacaga cccgacacca aagggaaaac gggaaaaacc ctcacttcgt gaactggaac
    72961 agcagataag acgggatatt gaggacggta tcgatagcac aggcaagaaa atgacgcttt
    73021 gccaactcta cgccaaacag aacgcacaaa gggcaaacgt gaagaaaagc acacagaaac
    73081 aacgggaaca actcatgcgg ttattgaaag aggacaagtt aggtgctagg agtattgata
    73141 cgataaaacc ctctgacgct aaagaatggg cgttacgcat gaaagacaaa ggcttttcct
    73201 ataacaccat taacaaccat aaacgctcgt taaaagcgtc attctatatc gccatacaag
    73261 acgattatgt aaggaaaaat ccttttgatt tcaagttaag tgaagtctta gaaaatgata
    73321 caaaagagaa agtcgcattg acagaggaac aagaacaagc cctactgtca ttcatcaaga
    73381 cggacaatgt gtatcataag tattttgatg atgtgctgat actgttaaag actggacttc
    73441 gtatctcgga actgtgcgga ttgacaatca tggacgttga ttttaaccat gaggttgtga
    73501 ttatcgacca ccagttacta aagagcaagg aacaaggcta ttatattgaa acgcctaaaa
    73561 caaagagcgg aacaaggcaa gtaccgttga gtgaggaaac aatcaaagca tttcaacgat
    73621 tgatgaagaa acgcccaaag gcagaaccat ttgtgataga cggacggggc aacttcctat
    73681 ttgtcaatca gaaaggcaag cccaaagttg ccattgatta caacgcctta tttgtccgta
    73741 tggtaaagaa atacaacaag caccataagg ataacccctt gccacatatc acaccgcaca
    73801 cgctacgcca tacgttctgt acaagactgg caagcaagaa catgaacccg aaagatttac
    73861 agtatatcat ggggcattca aatatcagta tcacaatgaa ctggtacgct catgcgtcca
    73921 tagataccgc aaaatcagag gttcagcgtc taattgcata gaagtattta ccacgatttt
    73981 aaccacgttt gatagcgaaa atataagaag atagacctag atatgtgagg tttaccacaa
    74041 aagcaaaatg cccgtagagc cgataaaata aggctttgcg gacatttaag aagatataaa
    74101 aagatagtca aaaagacata tataatttaa gataatttat atatatattt atctttctaa
    74161 aggcaaggat gtattcaact agaatagtta ctcaggaaaa tgtagatatt cctaatattt
    74221 cagttatgag ttcaatgaag gagtataaat ttgctaagga attattagaa gtatatggga
    74281 gtgaagataa gataaagcct cacaatataa tgtatattag tgcttggatt ttaggtatat
    74341 cagtaggaaa ttatgaagaa gatacagaag ataaggatgt aatttcaaaa attgtaaata
    74401 aaatgatgac aaagtttgga tatttaagcg gagtttacta tataagccaa gaaaaaatat
    74461 ttaaacaatt atattctcat tttagacctg cctactatag acttttattt aaattaccta
    74521 tttataatcc gatttgtgaa aaagtaaaag aagaatataa attagtttat agaattgtaa
    74581 gtaatgcaat gaaagagttt tgcggattat ttggagaaga aattcaggaa gaagagttag
    74641 catatcttac aatgcacttt gcaacacttt tttcaaataa aaaagaattt gatggtttta
    74701 gaaaaagaat agcattaata gtttgttcaa atggcgttgg tagttcagct attttacatg
    74761 cagaacttac aaacttattt cctgacttac actttatacc aatgtcggaa tcagttggat
    74821 tagaaaatat aagtgaacct gtagatattg tatttactac taattataac gcagagggtt
    74881 taaaaacaga tgtaccagta attaaagtaa gccctgttat gaccacaaaa gaaaaatata
    74941 agattacacg tgaagtgtat attcaattag gtgatatttt tttaagacaa cctaaaatag
    75001 atgaggttat ggatataata aaaaaatatg caaatgtaac ctctgaaagt attctttcaa
    75061 gtgagctatt agcttatttt acacagatag agaattttac atctaaggaa ggagaggggc
    75121 ctatgctaag tgagattact aatgaaacac tagtaaaaat aaaggtaaaa gctaagaatt
    75181 gggaggaagc tattagacag tcagcttctg tattagttga aaataacaaa gtaactgaag
    75241 ggtatgtaga tgctatgatt aactcagcaa aagcatctgg accatacata gtaattacaa
    75301 aacatgttgc acttccacat gctagaccag aagctgggtc aaaagagatt gcaataggca
    75361 ttgctacttt agatagtcct attgagtttg ggaataacga aaacgaccct gtaaaatatg
    75421 tattttgtct aagtgcaata gacaataata gccatttacg tgctatgtct gagctagttg
    75481 aactattgga agaagatgag ttttttaagg ttttagatag tgctaaagaa gctaaagaaa
    75541 ttattgattt tataaaaaca cacgaattat aaaggaggat ttaattatgt ataaaatttt
    75601 aatcgcatgt agagcaggag ttggctcaag tttaatgtta aaaataaaga ctcaacaaat
    75661 tattaaggaa aataactttc caattgaagt agaacatggt tcattggatt cactaaatgg
    75721 attcaatgga gatgctgtag aaacattaat tgatgttgct gaagaattaa aaaataaaaa
    75781 tttgaagttt gatataattg ggattcacaa tattgttgac cgtaatgaaa taaagacagc
    75841 actagaaaaa ttccttgctt caaaagaggg ataaatttaa gatgataagg aggaatattt
    75901 atggagttta ttattagttt gctgagtaca cctgctgttt tgcttggcgt agttgcatgt
    75961 ataggattga ttcttcaaaa gaaatcagct gttgaagtat ttacaggctc ttcaaaaaca
    76021 cttatcggtt ttttgatatt tggtattggt gcctctgcta tgacaggagc acttcaaaat
    76081 ttcaataaac tgttccaaca tggctttgga attactggtg ttatagcttc accagaagca
    76141 gctacagcac ttgcacaagg tacatatggt tttgcagttt catgtacatt aattcttgga
    76201 ttcatattaa atttagtatt tgcacgtatt acaaagttta agaatatttt ctttacaaca
    76261 ggtcatagtt tgttcttttc ttgtgtatta gttttaatta tgaaagcaca tggatttgat
    76321 aatacaatca ctattttaat aggtggaact atcttagggt ttatgtctgc tgcactacct
    76381 caattctgtc aaccatttat gcgtgaatta acaggtggcg acgaacaagc tattggtcac
    76441 tttaacatga ttggttatgg tttatcaggt tatattgggc gtttattttc aaaacataaa
    76501 gatgatacaa cagagactat agaatttcct aagtggttat caatattcag agacttctta
    76561 atgggtctat ccatagttat gttaatctta ttttatattg caacattaaa agctggtcaa
    76621 gcatttactc aagaaattgc tggttcaaca cattggttgg tttttccgct tatagaggca
    76681 tttacatttg tggcaggtat gtcaatattg atgtcaggag ttcgtatgtt cttagctgaa
    76741 ataactgcag catttgtaat catttcagaa aagtatattc caggttcacg tcctgcgcta
    76801 gatgttccaa ccgtatttcc atatgcacca aatgcagtta ttattgggtt tatatcagca
    76861 tatgctgctg gtctattagc aattgctatt atggcatcat ttcctagtat atttcctgta
    76921 gtaattattc cagctgctca catatgtttc ttctcaggag gtacagctgc aatatttggc
    76981 aatacttcag gaggatggag aggggctatt gctgggtcat ttgttgtggg gttattactt
    77041 gcatttttac cagttatact atatccagtt tatgggactc ttggtataga aggagcaact
    77101 ttccctaata ttgattacaa cgtagttggt tcgatattac ataatatttt acaatttata
    77161 aaacctattt agactaaatt atataaaagt atagtcatag ttactatgat ttcaaggagg
    77221 atacatctta tggaaaatac attatctgaa tttttagata atatatctaa tgaattgtca
    77281 gaatttattg cctcaataga tgagacatct ataaataaag catgcgaatt aattcttgaa
    77341 gctgaaaaaa atcacggacg tgtacatgtg actggtattg gcaaaccagg acatgtatcg
    77401 ggatatattt catcattgct ttcatctact ggaacgtcag catatatatt gcatggtaca
    77461 gaagcagtac atgggtcaag tggtcaagta gttgaaggag atgttgttat tgcaatatca
    77521 aatagtggtg aaactcaaga gttaaaagca actcttaaga ctctaaaagt aaatggagca
    77581 aagataattg gtgtaagtgg taatgagagt tcatttttaa aaaatatttc tgatatattc
    77641 ctgtttgcag gtgttaagca ggaaggagat tgtctcaata aagctcctcg tgcttcaatt
    77701 ttagctgaaa caataatatt acaatcatta agtgttgtat tacaatatgc taaaggtctt
    77761 aatacacagg agtatttgaa gtggcatcca gcaggaagtc ttggaaagtc tattagagag
    77821 ggaatataat tttatgaaat ataaagggat tatatgtgca atgataacac catttgatga
    77881 aaatcaaaat attaattcac aagcaacatg tcaattaatt gattatttga ttgaaaaggg
    77941 aatttatgga ttatttattt tgggcactaa tggtgaatgt catgtattaa cagatgatga
    78001 aaaagttgaa tttgcaaaga ttgttattaa tcatacaaat aatcgtgtac ctgtttttgt
    78061 tggaacaggg ggtaattcaa ctcgtgaagt tgtagattta tctaagagaa tggaagcgat
    78121 tggagcaagt gcattatcta ttattacacc atactttgtt acaccaacac aacaggaatt
    78181 aatcttacat tacaaaacta ttgctaattc agttaattta cctattataa tgtataatat
    78241 gccaggtaag actggaatta atattgaacc agagtcagta cgtgaattat caaagataaa
    78301 gaatattgtt ggaattaagg atagtagtgg taaacttgac aacatgaaag cttatattga
    78361 agcaacgaag aatgagaatt ttagtgtgtt ttcaggctca gattcactta ttctggatag
    78421 tctaaaagct ggtagccaag gagctgttgc agcaacaaca aacttcctca cagagattgg
    78481 tatctctatc tatgaaaact ttatgaaagg ggatttggaa gaagctcaaa aatctcaaag
    78541 tagtatagaa gaacttcgac gtatactgaa acttggaaca attccatctg ttatgaagaa
    78601 aactattgtc ttgaatggaa ttaatgttgg aactgcacga cttcctgtaa ctgaaccaac
    78661 tggggaagta cttgaagaaa ttaaacgagt agttgaaaat tataggacaa tacttaataa
    78721 aagtagtgag aatagataat ataaataggg aaattacact ggtatataat taatatatat
    78781 attataaaac atgactaaaa ttaatgaata agtaaagtgg tttgatgagt atagaggtga
    78841 taatatgaaa aagatgaata taaccaaaag aatgtcagaa tgtggggctc ttgcaattgt
    78901 aagagaagaa aatcttaacc gtgcatgtga aattgctgaa ggatgtataa aaggtggtat
    78961 aacagttata gaaatgagtt ataccctaaa taatgctgga gaaataattc aagggcttaa
    79021 taaaaagtat ggtgaaatac tttgtgtggg agcaggaaca gttttggata gtgaaacagc
    79081 tcgtcatgct atattacatg gagcccaatt tattattgca ccaaattata atgaaggtgt
    79141 agcgaagatt tgcaatagat atcagattcc ttatgctcca ggatgtacat cattgactga
    79201 ggcagtagac gcactaagtt taggagcaac ttttataaaa gctttcccaa tatcagattt
    79261 ctatggacct aaactagtaa aggtatttaa gacaccaata cctgatatgc cagttttagc
    79321 aagtggaggg cttaatattg aaaacctaca tatatggtta gaaaatggag ttgatgtatg
    79381 tggatttggt ggtttattaa ctaaaggttc tgttgaagat attgctaaaa atgctaaaaa
    79441 gattcgtgaa atcattaaaa atgtaagaaa aatttaacat agggctgtat catagtatct
    79501 tataattact atgatacagc ctttttaata ttattcaaat ttaaaacagt ttacatttct
    79561 attatattac atataatttc agtaatttta tcttaatttt tctatctgga aaaaaatgat
    79621 ttaaaactta agttatgata taatttcatt aaaagaaggc gatttttagt aaatatgaga
    79681 aaataatgtg tttttagggg aaaagttaaa tacagattta agtgaacaag aatcgataat
    79741 taagatatta aatggtgaat agggcatcaa aaaaattact aaattaaaag ttttagaggg
    79801 agaatcttag caagtgtgtg tgaaaattca atttcaaaca aaaataattg taaatgaaat
    79861 caatttatac acgttgaaga ttcactttta tcattatatt aaaaagcaga gtgaattaaa
    79921 agtgagtcta aagataggag gataattaaa acaatgaaag aagacattag gttacatcga
    79981 atgtgtaaaa aagaacaagc aacaaagaat gaagttatat cagaggatat tctaaaatct
    80041 atgagtattg gggtcagtaa acatttttta gatgattcat ttacaataat atggggaaat
    80101 acatcattct atcaattatt aggatataca gaagaagaat ttttagttaa attttctagt
    80161 ttaaaagatt attatgaaca gaatctttat cattttgaat ctatgaaaaa tcatttttta
    80221 gaggcttatc atcatggcga aaagagtata gaatatcaaa tttgcatttc agcaaataat
    80281 ggagaccctg tttgggtaat aatgactggt atattaattg aatcacaaga taatgagaaa
    80341 tcaatggtct atatattgta ttcaaaaatt aatgagttaa aaataaagca agaagaacta
    80401 tctgatatgg aaaaagagag tacagagaat tttaaatgga tgatgtcaga gtatgctggg
    80461 aatgtttata ttagcgatat ggatacatat gaattactat acttaaataa gcactcatgc
    80521 aatactttac aggcatcagc aaatgaactt attggaagaa aatgttatga ggtaattcaa
    80581 ggtagaactt cgccatgtcc gttttgtaca aattcgtatt taaaaaaaga tgacatatat
    80641 gaatgggaat tttataataa aaaactgaaa cgaacattta taattaaaga ccgtatgttg
    80701 aattggcaag ggcgaagagt tcgtattgaa ttatcttatg atatgtatag tgctgaatat
    80761 aaattagcaa aaaaagatca ggaaagagaa tcaattttga aaactattcc agcaggaatg
    80821 gttagattag atgcaagaga ttatcttact atactttggt gtaatgatat atttttgaat
    80881 atgattgaat atacaaagga acagtttgca gaggaactta ataatcaatg tggatattta
    80941 ttttctgaag attataaacg tagtaagaaa ctggcacaag atttgaagga gtctggggat
    81001 aatgttgtat ttgaagcaaa aatatataca cgttcaaggg aagaacgtat atggactgtt
    81061 acattgtgtt atatcagtgg ggaagacagt tgggatggaa taccatcttt ttatagtata
    81121 ggtattgaca taacaaagga aagaaaacag attgagaaat tacagcatat atcagagaaa
    81181 gatgcattaa caggaattta taatcgtgct gagactgaaa ggcagattaa gaaatatttt
    81241 gaaaaaaatt taaatgttat gggtgcactg tttatgattg atacagataa ttttaaacag
    81301 attaatgata cagaagggca tatgattgga gatatagtac ttactgagat ggcatcaggt
    81361 atgaaaaaaa taatgcgtga cagtgatgtt gttggaagaa taggtggaga tgagtttaca
    81421 atttttatga agaatatttc ctcagttaaa gatgcagaaa agaaagcaga agaactattg
    81481 tatatgttcc gtcatctatt tcagaaagaa aaaagttttc taaaagtaac ttgtagcata
    81541 ggaatagcta tatatccaaa ggatggtact acattcaagg aaatatatgc aagagctgat
    81601 aaagccctgt atcaagcaaa aaatatggga aaaaataatt atgtaatcta caaccaagat
    81661 tattttaaag aattagaaga atttgattgt tcttctcttg gtacagttat tgattcagaa
    81721 aaacaatacg ctgaatatcc tgataatctt acacgttata tattccgtat gctttatcag
    81781 actgatgata tagaccaggc gattaatatg atacttgagg ttgttgggaa acagtttgat
    81841 gtaagtcgtg tttatatttt tgaaaataca gaagatggta gatatacttc taatacttat
    81901 gaatggtgta atgaaggaat ctcttctcaa atggcttatt tgcaaaacag caattatcaa
    81961 gactatgagg attatgaaaa gctttttgag gatgaattag ttttttattg tcgagatatc
    82021 catacattaa gtcctaaact agaagaattg ttttctagtc aaggtatcca ctctacactt
    82081 caatgtgcat ataaagaaga tagagttttc agtggatttg ttggatttga tgaatgcaca
    82141 ggactacgtc tatggtcaca agaggaaata agtacattat cattgatttc tcaaattata
    82201 tcaatgtttt tacaacgtaa aaaaaataat aaacttaatc aacagattca acaataccag
    82261 aatattttag ataatattga ccagtgtatt tgtgtgatta ataaagaaaa taatttttta
    82321 ttatatgtaa ataaaaaatt taaaaataca tattctaaat tccaaattgg acaaccttat
    82381 tatcttgatt ctatagatat tcataagata tccattatat ggaataaaaa agaagcatac
    82441 ctatgtacta gcacgattta atggtaaagt aaagtttata ttggacttaa attaaatttc
    82501 gttttaaata agatattatt ttagttgctt actgaaaaga ctagaatcta tattaatgaa
    82561 ttatactaat tttataccac tattaaaata ttgtttgcta aaaataaaaa atagccttaa
    82621 aacagttgat tataaaaaat taactgcttt aaggctattt ttatctttgt gacgaaatag
    82681 acacttgcca tttagaatgt gacaatatag tagttgttta gaaaaggcac aaagcattat
    82741 actttaacta aagtaaaatg tattttgctt ttagaataaa aacaattagg agggagctat
    82801 aattgaaaaa gataatatat gtattattta caattgaact attaatagta tctttattag
    82861 gtctaaatat agttaaagat aatgaaatta ataatatttt atataacgat acaacaagta
    82921 tatctgtaat gtttaaagat tataaaaagt taaataaaaa ttatagtaag tggattaaag
    82981 atattgcaga tgaaaataat gtggctatat caaagtatgt ttttaataat aatgaaaaat
    83041 tatctatata tacgactgat acaagtttga ataacaatat aaagttaaaa tcaggaaaat
    83101 ttcctaatac taattcaaat gaatttatta gtaatataaa cactaataat agtaatcaag
    83161 taggtaaatt ctcaactatg agtaaagaca tatcaatttt aattagggat tttggtaaaa
    83221 taagtgaagt tggagaatca ggaatcttat atatttcaac tcaaaatgaa gatacaataa
    83281 ataaaatatt aagagaacta aatgtagaca gaagtatttt tgtagcaaga ttacatgata
    83341 gatatgttaa ctcaaattta tacttaaatc cttcaataat tagagattta atactaatta
    83401 tcctttgttt cttggctaca ataatacatt attcaattag tgtgagtaga gaggcttcta
    83461 ttttaagatt aaatggatac tctaagctag atataattgc tagagttata aaaaatatac
    83521 ttagaattat gattttatca tctgtcacag cattattaat atatataatc tatattttaa
    83581 aattaaatat aggtgttaga gcatgcttat actttgctat gttttcgatt atatgtattt
    83641 tgataaatat tttgatatct atattaatag ttggttttaa tagtagaagt tctaaatatg
    83701 tgctatctct aaatggaaaa aaatcttatg gttttgtaaa tataatgcat ttcacattga
    83761 aaattgtatt tgtatcattt cttatattat ccataaataa ctgtatttta aattataata
    83821 tgctacaaac tcagttagga aatctatctg agtgggataa agcaaaaaat gtttataatc
    83881 ttactcttaa agatacaggc gaacaatctt taggaaagga agaagtaatt aaaaatgcca
    83941 agattaaagg tttctataaa aatttggtag aagaaaaaga tgctttctta atagattcaa
    84001 ctaattttga aaaacttgaa gatggaagtt acatgtatga aataaattca aagggtaaaa
    84061 acccagaagt aagtcaatat ggtaaaaata taaaaataaa taaaaactat cttaaagtta
    84121 accctattta ctcaaatggc aaagaaatat ttaatctaat cgattatgac gataagacta
    84181 taaatttatt agttcctaaa aagttacaag tacatgaaaa tgagattaaa aaagagttta
    84241 gagaattatt ttattttgaa aaaatagaag tagaaaatat gtacaataaa gagttaaata
    84301 gagagttaaa taaaacaaag aagtccgatt taaatgttaa tataatatat gtagataata
    84361 atcaaagcta ttttacttat aactcccttg ttatggatga caataggaat ctcattgatg
    84421 acccaatagc aatagttgac atagataata ttgatgattc tttttatctt tcttatatat
    84481 ctagatgtgt atattttaat tctaagaaat tagatgccct tgcagatata agtagcatta
    84541 ttgaagctca aggtgtagaa gcgcatatcc aaagtttaca tgctgtttat aatgagtatg
    84601 gtttagaaat aaataaacta gaaaaatatt tgaatagtga aatttttact ataataataa
    84661 tagcaatatc aaacttaatg attacctaca atatagtagc tagctactat gaaagaaata
    84721 aatataagtt atatattaaa aaaatatttg gttactcaac tacagctaga agtatgttgt
    84781 taatagtctc attaatatta acaaacatta ttccaatagg cattatatca acaagagtag
    84841 atttaagtaa taatattatc ttatttggac ttctaatatt agttatagaa gtaattgtat
    84901 ctattgtatt agacaagatt atctcaaata gttcatttaa taaaattata aaaggagaac
    84961 attaaaaaga tgatttcatt aataaatata tctaaaaaat ttaaagataa atctataata
    85021 gacaaattta acctagaaat ttctaagggc gagtttgtag cgataacagg aaatagtggt
    85081 tcaggtaaga caactttact taatataatg ggattattag aaaaacctaa ttctggtgat
    85141 attataataa ataatataaa aaatccaaat agtaaacaga taagaatttt acaaagggat
    85201 ttttatggct atctatttca aaattatgct ttaattgaga atgaaaatgt tgaaaataac
    85261 ttgaaaattg ctttaaagta tcaaaaaaat atcaatcata atgaaaaaat aaattacgca
    85321 cttgagtctg taggtcttag aaaatacaat aaaaaaaaga tatacgaatt gagtggagga
    85381 gaacaacaaa gggtagcatt agcaagagta atattaaaag aatgtgaagt aatatttgca
    85441 gatgaaccta caggtaattt agataaaaaa aatagagaca tagtatttga tatacttaaa
    85501 agtctaaata agaatggtaa aactatcgta tttgtaactc atgatttaga tttatcggag
    85561 caggcagata gagttataaa attgtaataa tataaactat aataaatata agaaagagat
    85621 tacagctaaa ttaagtttaa taataatacg aatagaattt aaacattaag taaattagta
    85681 gtttgtaaat ataagtataa tatgatatca taaaggctca aaagaaattt tatataataa
    85741 gaaatctaaa aataacaaat ggaggacata taaagatgga aaatttacca aattgcccga
    85801 aatgtaattc agaatatact tatgaagatg gaactctttt agtttgtcca gaatgctctc
    85861 atgagtgggc attaggattg ggagctgaag aagattcaaa tattatcaaa gatgtaaatg
    85921 gaaatgtatt aaatgatgga gattctgtta cagtaattag agaccttaaa gtaaaaggaa
    85981 gttcatcatc tataaagatt ggaacaaaag tgaaaaatat ccgtttaatc catgactcat
    86041 cagatggaca tgatatagaa tgtaagatag atggttttgg agctatgaag ctaaagtctt
    86101 ctgttgttaa aaaagcgtaa tattataaat aagctgtagt gtttattgtt tttcagtaca
    86161 gtataaaacc ataagaatct attaagtaat aaaagtttgt ttatttatct ttatattttc
    86221 ttatggtttt attaaatata aattttattg aacaccaaaa tattctctcc tctacaaata
    86281 agccaatctt atttaaacta atatactttg tactatttat actgaaattg taaatttaaa
    86341 tgctatgtta tttaggtttg tatagttaat ttatatattt ctgattttat aaattgtatt
    86401 tattattata tcttgattct aaaatcatta aaaatttcaa cttctataaa gatttacaat
    86461 ttatcaaata cattatgagt atataaacaa tttattatta cagtgctaca tttaaaataa
    86521 gtcttagtat aaaggtattg ttacaagtat aaagaaactt atgtatgcta ttggaataaa
    86581 taatacctta aattataatt aagaataaga actgttaaat aagggacttg attatcattt
    86641 agttatagat aatataatat taaaaataaa gtttaagagg agaaaaaata tgatagatat
    86701 acataaaaat atatatgata ataagttatt tgaggagcta aagattgact gtaagaagtg
    86761 ctttggactt tgctgtgtag ctctttattt ttctgcttca gatggatttc ctatcaataa
    86821 ggaatcagga aaaccctgta ttaatttaca accagatttt aaatgttctg tacataatag
    86881 tcttattaag cgaggtttta aagggtgtac tgcatatgat tgttttggtt caggtcaaaa
    86941 agtagcacaa gtaacatata aggggataga ctggatgcaa tcaccagaac taacaaatca
    87001 aatgtctgaa gtgtttttaa ttatgagaca attacatgaa atgttatggt atcttaaaga
    87061 agcatctgta ttaaatatat cagatacaat caaatcaaaa attgatttaa taattgaaga
    87121 aactgaaaag ataactaata tgggcccaga acagataatt aatttggata tcatatcaca
    87181 tagaacaaag gttaatttat tattatcaca agcgagtgag tctatgatgg gaaaggttaa
    87241 atcatttatc aagacttcta ctcttaaaaa catgaaaaaa ttaagtaaaa atatagattt
    87301 aataggagca gaccttagaa aaataaatct aataggagca gatttaagag gtagatttct
    87361 aatagcagca aatttaagaa atacagattt gagtggggca aatttaatag gagcagactt
    87421 aagagattgt gatattagag gtgctaatct tgaaaatagt atatttttaa cacaattaca
    87481 agtaaatact gcaaaaggag attctagtac gaagttacca gcttcactaa tacgccctaa
    87541 atactgggaa aaataattaa attaatacat aattttacaa aatatgaact tttttgactt
    87601 taattcatat tatacattag agattcttta caagaatttc ttaatataat atagggagag
    87661 atataaaaat gagttatcaa aattataaat gtgaaagaga ttatgtgtat gatgattata
    87721 aatacaataa gtgcaacaag tgctataatg attatgagga aatgacacat gttcatgaat
    87781 attcagaaag tgttaagtta gcagaagaat gtgaggatag acataaccac cgtgcagcag
    87841 gggttactgg tgaagttata ccaataaatg gaggtacaaa tcatgttcat aaaataaatg
    87901 ataatgtaga tttccttgac catttccata agatatgtgt tacaacaggg ccagcaatta
    87961 gaatacctgg aactgataaa cacattcact taatatgtgg agaaactact gttaatgatg
    88021 gtcattgtca taaattcctt tttactactc aaatagaagc acctttagta taagctgatg
    88081 tgaattattg tagtgaattt gctagattat agtataatat ataatagata atatttaaac
    88141 ttaatgagga ctatgatatt taaccttcgt agtcctttct aagttttgta taaaaaagtg
    88201 ttatgcaatt aaaagtgata tagggaggaa atttttatga aatctggaat aattcttgaa
    88261 atgaaacaaa taaattcaac agaatatgac gaaatgttgc aagtctggga atcatcagta
    88321 agagcaacgc atgatttttt aacagaaaaa gatattgaat ctttgatacc attagttgag
    88381 attggactta aagaagtaga aaatatagtt tgtataaaag ataatgatat aataaagggc
    88441 tttataggaa tagataaaga taaaattgag atgctcttta tagaagataa atatagagga
    88501 aatggtattg gtaaaaaatt aataaaatat gctatagata aatacaatgt aaaatatgtt
    88561 gatgtaaatg agcagaataa aaaagctgta ggattttata ttcacttagg atttaaagtg
    88621 tttgataggt ctgaaataga tggacaaggg aacccatttc caatactaca tatgaaatta
    88681 atttaaagtt atacttccca cttattcgca aaaatattta cttaaatttt tatacaaata
    88741 aaagtcgtga cagtcttagg acacgacttt tttactacac tattaaattg aaatttatct
    88801 gttctatatt atgattatta gatgtagttt ttttatttta actatgtact taaataccaa
    88861 gtatacccat acctatcaag tacttcagca gcgccatcat tttgatgtgg tggtgggtgt
    88921 ggtaaaaatt cttgtacaag tgtaccacct tcggcaagtt ttacagatat ttcttctatc
    88981 gcttctcgag atggtagaac aacacaaatc gccatgccag aaattacaac aggcttgtct
    89041 gtatccgcac ccattataca accttcgtat tttgtgaaag taacacttgc gtgcataata
    89101 ggattgtttg gattgctgtc taatcgcgca aatatagttt ttcctcctcc gaaagcctca
    89161 gcataaaaat taaatgcttt ttcacaagta ccattaaata ttaaatatgg agttagcatg
    89221 accaatatat cctccttgat atagaataat ttatagttgc acctgataat ctaaattatg
    89281 atttagtaaa ttgtatcata gggaaacctt gaagtatata attttttatt attatattat
    89341 acaaatttat taatttaaaa aaatgttata tgtacggaag gtttacagga taaaatataa
    89401 taaaaagatt aaggtgacat tataaaaaat gtatattgac attgacgcta cgtaattgta
    89461 tatactttgt cttaaaggag atggtaaata tgaatattat agaaattcgc tcagataaaa
    89521 tatacaagaa gataatggat gcaccaataa acaaaaaaga agatatatac agatatgaat
    89581 taatgaagcc ttttgaattt aagtggaagt gtatgaatgt tccaatagtt gctagacaga
    89641 aaggtggata tgatgtaatt atagcaagtg aaatgttagg ggttttatcg cctaaggata
    89701 ttgatgaaaa gcaaaaaaag aatataaatg tgttatctgc tgataaaatt tggggcactt
    89761 gcaaagaaac catagaaaac tctataaatg cttttataaa agaagggtat gatttaaaca
    89821 ttaaggacta taaatattca atattattgg cgaatccaaa tagtccttat acaatattaa
    89881 gtgatggata ctggggtgat ggtgggattc ctggatatat atttctatca ttggttccta
    89941 atgaatatac tatcaataga ttaccagtat taatagcaca tgaatgtaat cacaatatta
    90001 gatttcagtt tatagagtgg aataataata taacattaga agaaatgatg ataaatgaag
    90061 gtcttgcaga aaattttgca acatggatgt ttggagagga aatgttagga ccttgggtca
    90121 gtagaacaga tatcgaaaca ttaaatactt atataaagcc aataataaaa agtgctttaa
    90181 aagaaactgg atttcaaaat ataacatctt atctttatgg tgatgatata gctaaaatgc
    90241 aaggatattt tccagtaggg ttgccttatt gtgcaggata tgcttgtgga tattatatga
    90301 ttaagtatta tttagaaaag acaaataaat caataatcga agcgacttta ttgccttata
    90361 gtgagataat cgaagcagta aaagagtttt gggaataaat tttataaatt gcataatttg
    90421 aaataaatta tgcaattatt ttatttttaa gaaaaaataa attgacaata atttataaga
    90481 aatatacaat aatatatata aagaaatatt tcaatattta aaagttttaa gatttcatat
    90541 ctagagtagc taacattaca tgttagtttc tt
//
LOCUS       DAMJYC010000005        84732 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJYC010000005 DAMJYC010000000
VERSION     DAMJYC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 84732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.005"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(170..3505)
                     /locus_tag="PC472_000351"
     CDS             complement(170..3505)
                     /locus_tag="PC472_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2725720.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYTNDSDLLLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3555..3983)
                     /locus_tag="PC472_000352"
     CDS             complement(3555..3983)
                     /locus_tag="PC472_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725721.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLTCEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3973..4353)
                     /locus_tag="PC472_000353"
     CDS             complement(3973..4353)
                     /locus_tag="PC472_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2725722.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTKALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4386..4694)
                     /locus_tag="PC472_000354"
     CDS             complement(4386..4694)
                     /locus_tag="PC472_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725723.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4727..5827)
                     /locus_tag="PC472_000355"
     CDS             complement(4727..5827)
                     /locus_tag="PC472_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2725724.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVKVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            complement(5831..7294)
                     /locus_tag="PC472_000356"
     CDS             complement(5831..7294)
                     /locus_tag="PC472_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2725725.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(7804..8673)
                     /gene="gatY"
                     /locus_tag="PC472_000357"
     CDS             complement(7804..8673)
                     /gene="gatY"
                     /locus_tag="PC472_000357"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2725726.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(8693..10609)
                     /locus_tag="PC472_000358"
     CDS             complement(8693..10609)
                     /locus_tag="PC472_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2725727.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10612..11532)
                     /locus_tag="PC472_000359"
     CDS             complement(10612..11532)
                     /locus_tag="PC472_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2725728.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11634..12386)
                     /locus_tag="PC472_000360"
     CDS             complement(11634..12386)
                     /locus_tag="PC472_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2725729.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12778..14223)
                     /locus_tag="PC472_000361"
     CDS             complement(12778..14223)
                     /locus_tag="PC472_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2725730.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(14245..14598)
                     /locus_tag="PC472_000362"
     CDS             complement(14245..14598)
                     /locus_tag="PC472_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2725731.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(14627..15874)
                     /locus_tag="PC472_000363"
     CDS             complement(14627..15874)
                     /locus_tag="PC472_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2725732.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(15882..16199)
                     /locus_tag="PC472_000364"
     CDS             complement(15882..16199)
                     /locus_tag="PC472_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2725733.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16441..18354
                     /locus_tag="PC472_000365"
     CDS             16441..18354
                     /locus_tag="PC472_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2725734.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(18477..19631)
                     /locus_tag="PC472_000366"
     CDS             complement(18477..19631)
                     /locus_tag="PC472_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2725735.1"
                     /translation="MKMLISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(19910..22525)
                     /gene="mngB"
                     /locus_tag="PC472_000367"
     CDS             complement(19910..22525)
                     /gene="mngB"
                     /locus_tag="PC472_000367"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2725736.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(22669..24579)
                     /gene="mngA"
                     /locus_tag="PC472_000368"
     CDS             complement(22669..24579)
                     /gene="mngA"
                     /locus_tag="PC472_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2725737.1"
                     /translation="MIRNLVSFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEYLEAVM
                     YRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELIFLIAV
                     PESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKEEIRIQ
                     NESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHTPDIIK
                     RADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKGDYIEK
                     SSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQELYNQEG
                     SWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGFLGGML
                     GGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAALNNGL
                     INWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAIFASVK
                     MVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVIPSLVA
                     GSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIISAILLI
                     ITRKQKNQRKIH"
     gene            complement(24730..25920)
                     /locus_tag="PC472_000369"
     CDS             complement(24730..25920)
                     /locus_tag="PC472_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2725738.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(25998..26201)
                     /locus_tag="PC472_000370"
     CDS             complement(25998..26201)
                     /locus_tag="PC472_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2725739.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(26574..26828)
                     /locus_tag="PC472_000371"
     CDS             complement(26574..26828)
                     /locus_tag="PC472_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2725740.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            complement(26831..27250)
                     /locus_tag="PC472_000372"
     CDS             complement(26831..27250)
                     /locus_tag="PC472_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2725741.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            complement(27517..27747)
                     /locus_tag="PC472_000373"
     CDS             complement(27517..27747)
                     /locus_tag="PC472_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725742.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            complement(27771..27962)
                     /locus_tag="PC472_000374"
     CDS             complement(27771..27962)
                     /locus_tag="PC472_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2725743.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            complement(28126..28842)
                     /locus_tag="PC472_000375"
     CDS             complement(28126..28842)
                     /locus_tag="PC472_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2725744.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            complement(28876..28965)
                     /locus_tag="PC472_000376"
     CDS             complement(28876..28965)
                     /locus_tag="PC472_000376"
                     /inference="COORDINATES: protein motif:HMM:NF025568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725745.1"
                     /translation="MIIFCPECKHNFLISAEELKMNIVISHKY"
     gene            complement(28962..29588)
                     /locus_tag="PC472_000377"
     CDS             complement(28962..29588)
                     /locus_tag="PC472_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2725746.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNVLYQKCLVNGPISYIEDIFNEMIKKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKSQLTTHVNQFVKKYITIEKVK"
     gene            complement(29880..30020)
                     /locus_tag="PC472_000378"
     CDS             complement(29880..30020)
                     /locus_tag="PC472_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725747.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            complement(30072..30983)
                     /locus_tag="PC472_000379"
     CDS             complement(30072..30983)
                     /locus_tag="PC472_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2725748.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            complement(30995..31693)
                     /locus_tag="PC472_000380"
     CDS             complement(30995..31693)
                     /locus_tag="PC472_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2725749.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLRAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            complement(31690..32373)
                     /locus_tag="PC472_000381"
     CDS             complement(31690..32373)
                     /locus_tag="PC472_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2725750.1"
                     /translation="MRLGRLIRGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFA
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            complement(32370..33215)
                     /locus_tag="PC472_000382"
     CDS             complement(32370..33215)
                     /locus_tag="PC472_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725751.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            complement(33319..33900)
                     /locus_tag="PC472_000383"
     CDS             complement(33319..33900)
                     /locus_tag="PC472_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2725752.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVIFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            complement(33978..34835)
                     /locus_tag="PC472_000384"
     CDS             complement(33978..34835)
                     /locus_tag="PC472_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2725753.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIETFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            complement(35048..35517)
                     /locus_tag="PC472_000385"
                     /pseudo
     CDS             complement(35048..35517)
                     /locus_tag="PC472_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(35533..35730)
                     /locus_tag="PC472_000386"
     CDS             complement(35533..35730)
                     /locus_tag="PC472_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725754.1"
                     /translation="MLKQGAKLSGVPLSEIQENIEATIDEAMNSTDPEVQANFKKYFG
                     NKRPTPEEYIYTMTKKSRFKV"
     gene            complement(35844..36461)
                     /locus_tag="PC472_000387"
     CDS             complement(35844..36461)
                     /locus_tag="PC472_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2725755.1"
                     /translation="MKNNIMLILPIAFILDLLIPFILAPTYRGYSHLTQVMSVLGNSK
                     APLHNVYNTWLVIFGMTLAVINFKVYAVVSKGSRLIAIILFIVLLIYAIGGCILSGLF
                     SVGEVKSLNTLSEKIHGYGSVVGFMCLSFAPLLLGIYAYKINDIKFSFFCIGCFILAI
                     LCFSCFVMGDKPNFRNTFLAFEGLWQRLSLLFMYFPLGCLILFET"
     gene            complement(36451..36690)
                     /locus_tag="PC472_000388"
     CDS             complement(36451..36690)
                     /locus_tag="PC472_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2725756.1"
                     /translation="MPMKKGQHIFGTVKVGEKGQIVIPKEARQIFDIKAGDTLLVLGD
                     EEQGIAIVKADVMKELAVKILKGLGKLSNGIDDEK"
     gene            complement(36823..37677)
                     /locus_tag="PC472_000389"
     CDS             complement(36823..37677)
                     /locus_tag="PC472_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725757.1"
                     /translation="MRTVKVGTHKKSVIALWVVLIASVSFGVYKNFTAIDMHTVHETE
                     TIQLRLNDTNGIENFVKNFCKSYYTWDNSKEAIEARAQAISNYLTKELQDLNLDTIRT
                     DIPTSSIVTNVLIWDIEQSGTDDFIATYEVDQQIKEGEQTTNVKATYTVKVYVDKDGD
                     MVIVQNPTLAPAMEKSDYEPKAQEADNSVDADTTADATAFLETFFKLYPTATETEIAY
                     YVVGNALEPINGDYLYSELVNPVFTADEDNIKVKVAVKFIDNQTKATQISQYELTLHK
                     DGNWKIIG"
     gene            complement(37731..38347)
                     /locus_tag="PC472_000390"
                     /pseudo
     CDS             complement(37731..38347)
                     /locus_tag="PC472_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(38614..39165)
                     /locus_tag="PC472_000391"
     CDS             complement(38614..39165)
                     /locus_tag="PC472_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2725758.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKNEKNPELLFNKPEKATKSFHWGIVHKQDSKVIGELWIYLIENDRMAKVAFRL
                     SKQYQGNGLMSEVLKGVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRE
                     GKMVNTYCDYYLYGILKSDYIIS"
     gene            complement(39179..39655)
                     /locus_tag="PC472_000392"
     CDS             complement(39179..39655)
                     /locus_tag="PC472_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2725759.1"
                     /translation="MTTYKVNSIGTIYNNEKGAFIQLEAPYIPALKELDGFSHINVLW
                     WFSDFDNADFRSVLETDQPYKKAPEKMGIFATRSPVRPNPIALSAVEIINIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWENEFNF"
     gene            complement(39736..40551)
                     /locus_tag="PC472_000393"
     CDS             complement(39736..40551)
                     /locus_tag="PC472_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2725760.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALACSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKELLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCTIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            complement(40654..41556)
                     /locus_tag="PC472_000394"
     CDS             complement(40654..41556)
                     /locus_tag="PC472_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725761.1"
                     /translation="MFKKNKKQTENIKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDMHTIHETETIQLRLTDTNGIENFVKNFAKSYYSWNNSKEAIDARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSAVTDVIVWNIEQFGTDIFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDADGDMVIVQNPTLAPAIQKSDYEPKTPEADGKLDADTINDATAFLE
                     TFFKLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPILTKDGDNVKVKVAVKFLDK
                     QTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(41576..42580)
                     /locus_tag="PC472_000395"
     CDS             complement(41576..42580)
                     /locus_tag="PC472_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2725762.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLISADTDDDNSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ENFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQISGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQTQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     "
     gene            complement(42577..44763)
                     /locus_tag="PC472_000396"
     CDS             complement(42577..44763)
                     /locus_tag="PC472_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2725763.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTMAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNENNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTISNGKPTRKTSTKDSRGNTSSSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVHSAKEKAKTSVSDFKRGVVQ
                     EQQSRQTGRSEKLEQHKKNIADKRMELQKAQEARQARKMDGSATTGATRSHERPATAL
                     KPSVEKTQEVKRPATTSTSKVSEPVKTNGVKERPLSSGASDRKTTQSAQPVYRQNIEK
                     VVSQETRQNYKTERTTKTQTFEQSKHTTEHTEKNRNLVTKKGQKKK"
     gene            complement(44763..47213)
                     /locus_tag="PC472_000397"
     CDS             complement(44763..47213)
                     /locus_tag="PC472_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725764.1"
                     /translation="MYPIKYIDNNLVWNKDNEVYAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLREVAVRKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIDDFGYLMEHLYGRDGIAYEDYEYQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKVVRSVTQSETRGLLHVIDELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(47200..47589)
                     /locus_tag="PC472_000398"
     CDS             complement(47200..47589)
                     /locus_tag="PC472_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725765.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFFI
                     ILFGDMPPLSMVEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQILNETITAVRSVNYVPD"
     gene            complement(47699..48202)
                     /locus_tag="PC472_000399"
     CDS             complement(47699..48202)
                     /locus_tag="PC472_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2725766.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQC
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIEELPDYIVECLDEFIGHYGTLEEVMEH
                     KDNIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(48220..48723)
                     /locus_tag="PC472_000400"
     CDS             complement(48220..48723)
                     /locus_tag="PC472_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2725767.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVYENLEALYICR
                     NVIAVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLLENRRYLVTEH
                     AVFELPE"
     gene            complement(48642..48938)
                     /locus_tag="PC472_000401"
     CDS             complement(48642..48938)
                     /locus_tag="PC472_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2725768.1"
                     /translation="MNDIFKDMQAKVGCENISDLPSYKRKVWHEMKQLNFADYEEKQL
                     EDFSNYVFGMSCQTLKDVMKQQKGREEQCRKQGCWWKRAEQLAKKQHHTGSTCR"
     gene            complement(49042..49584)
                     /locus_tag="PC472_000402"
     CDS             complement(49042..49584)
                     /locus_tag="PC472_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2725769.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNHCGGCGNGNQSCKIARC
                     SLEHGMIEYCYECKQYPCEKYQHIDDYDIFITSRRQKADLERAKIIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EMFQKIADRRNIKLKLIKKK"
     gene            complement(49659..50189)
                     /locus_tag="PC472_000403"
     CDS             complement(49659..50189)
                     /locus_tag="PC472_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2725770.1"
                     /translation="MCKILLVRPSKQYADQVMSYKEEMSQNGDSFDGCAGLENVQSFE
                     EWIDFEARLKAKYKDGYVPSEVFLAIRKKDNYVIGMIDFRHPLSDFLFNFGGNIGYSV
                     RPSERQKGYASEMLRLILPICHEFGESKVLLTCDKKNEASQKTIVRNGGVLEKEIPDT
                     VGLSESGIIQRYWISI"
     gene            complement(50273..50494)
                     /locus_tag="PC472_000404"
     CDS             complement(50273..50494)
                     /locus_tag="PC472_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725771.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(50495..50629)
                     /locus_tag="PC472_000405"
     CDS             complement(50495..50629)
                     /locus_tag="PC472_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2725772.1"
                     /translation="MWELLKDLLLVSFGIGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(50637..51836)
                     /locus_tag="PC472_000406"
     CDS             complement(50637..51836)
                     /locus_tag="PC472_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2725773.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLVAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCEHEECISKFRSFKSYGSGELVKHNETDKYD
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPESY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(52038..53432)
                     /locus_tag="PC472_000407"
     CDS             complement(52038..53432)
                     /locus_tag="PC472_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2725774.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSKEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(53549..54034)
                     /locus_tag="PC472_000408"
     CDS             complement(53549..54034)
                     /locus_tag="PC472_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725775.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQVFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(54132..54515)
                     /locus_tag="PC472_000409"
     CDS             complement(54132..54515)
                     /locus_tag="PC472_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725776.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(54531..54857)
                     /locus_tag="PC472_000410"
     CDS             complement(54531..54857)
                     /locus_tag="PC472_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2725777.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(55093..58137)
                     /locus_tag="PC472_000411"
     CDS             complement(55093..58137)
                     /locus_tag="PC472_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2725778.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTASHNEISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYKCGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDNSGNTEVAEVKATTVYIKELTAPMGFKVDKT
                     IYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYTKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTCEETKSPTGYLLDGAYMQANGSEEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFAITENGKIVDLTDEAHSV
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DDKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            complement(58602..59327)
                     /locus_tag="PC472_000412"
     CDS             complement(58602..59327)
                     /locus_tag="PC472_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2725779.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(59590..60369)
                     /locus_tag="PC472_000413"
     CDS             complement(59590..60369)
                     /locus_tag="PC472_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2725780.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(60362..61789)
                     /locus_tag="PC472_000414"
     CDS             complement(60362..61789)
                     /locus_tag="PC472_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2725781.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            62180..62902
                     /locus_tag="PC472_000415"
     CDS             62180..62902
                     /locus_tag="PC472_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2725782.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            63099..64766
                     /locus_tag="PC472_000416"
     CDS             63099..64766
                     /locus_tag="PC472_000416"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2725783.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(64862..66181)
                     /locus_tag="PC472_000417"
     CDS             complement(64862..66181)
                     /locus_tag="PC472_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2725784.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(66222..66968)
                     /locus_tag="PC472_000418"
     CDS             complement(66222..66968)
                     /locus_tag="PC472_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2725785.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(67212..69221)
                     /locus_tag="PC472_000419"
     CDS             complement(67212..69221)
                     /locus_tag="PC472_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2725786.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKRNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(69659..71092)
                     /locus_tag="PC472_000420"
     CDS             complement(69659..71092)
                     /locus_tag="PC472_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2725787.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYKNVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYAFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(71108..72532)
                     /gene="ascB"
                     /locus_tag="PC472_000421"
     CDS             complement(71108..72532)
                     /gene="ascB"
                     /locus_tag="PC472_000421"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2725788.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDIWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(72619..74535)
                     /locus_tag="PC472_000422"
     CDS             complement(72619..74535)
                     /locus_tag="PC472_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2725789.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(74609..75469)
                     /locus_tag="PC472_000423"
     CDS             complement(74609..75469)
                     /locus_tag="PC472_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2725790.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKRRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(75820..77007)
                     /locus_tag="PC472_000424"
     CDS             complement(75820..77007)
                     /locus_tag="PC472_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2725791.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(77021..78412)
                     /locus_tag="PC472_000425"
     CDS             complement(77021..78412)
                     /locus_tag="PC472_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2725792.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(79007..80707)
                     /locus_tag="PC472_000426"
     CDS             complement(79007..80707)
                     /locus_tag="PC472_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2725793.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(80679..82082)
                     /locus_tag="PC472_000427"
     CDS             complement(80679..82082)
                     /locus_tag="PC472_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2725794.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(82346..82837)
                     /locus_tag="PC472_000428"
     CDS             complement(82346..82837)
                     /locus_tag="PC472_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2725795.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(82863..83675)
                     /locus_tag="PC472_000429"
     CDS             complement(82863..83675)
                     /locus_tag="PC472_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2725796.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(83688..84647)
                     /locus_tag="PC472_000430"
                     /pseudo
     CDS             complement(83688..84647)
                     /locus_tag="PC472_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT        25551 a        16809 c        11308 g        31064 t
ORIGIN      
        1 ttcatagtta tttatcaatt aaaaagagat attgcataat tattcaatac ctctctttgt
       61 atatttacat tatccatatc tagttatcta tttcaaccca atattagact acctactaat
      121 aaatttattc tctacatcat taacaataat ttttcactaa ataacatcat tagcctatct
      181 ctgatgcatt ttctttaatt ttatttatag ttttaatcaa tttatttctc tcttcaatat
      241 gattttcaaa taaatcaatt atacttccaa cagttctaaa tgggtcttcc atcaacactt
      301 tgttatcctc tataaatcca ttcttgacta cataatcgac tataagttta acaaaatgta
      361 tttgcttagt attaaagctc ttattattta aaaactctga aaaagcttca tttgcagctt
      421 ctctatccaa tcccactagt tttctcacta attgacttac tggagtatct ccaaactcct
      481 tcttataatc atcacaatta cctaactgtt ttaagagtat ttcctccaac gtttcaacat
      541 cttgctttgt tatcttttta ttatttctta atttaaatat tgctaattca tctttatggc
      601 tatttaaata atactctacc ttctttttat aattcttaag attgtttgca ttatatacag
      661 aatcatttct atcttctgct acaatcatat cttcaaaact tgtataatat atcttttgac
      721 tagacttttc aagatactta ataagctctc ttaatgatat acgtacttct tctacctcaa
      781 atatatctga atcttcccaa aaatcctctg tcattgcttt ttcaatagta tatttttgtt
      841 gctgaatctg tggtatagtc ccaagttttg aaagtttttc tgctgtagcc attacatgcc
      901 ttataccttt acttgcatta ttattttgta agtatgcaag ttgtatatta tacataacta
      961 catcaaatct tttagccaat tcatcatcac tatatgaagt tatgagtggt gatatatatt
     1021 ctcttatctc attttgtgct actgctccaa tattgctcca aacattttca tttttatact
     1081 tatgtacata acttaaattc atcctaacta tataactatc ctcatttaaa gcttttacag
     1141 aagttataag ttcttctaac aattcttttc taagttctgc atactcacct tctatatatt
     1201 taatatcttg aagttcttta accatactta cctttatatt aaataatttt tcagtaagtg
     1261 tttctgtttt atttccctca aatcctttag gattaaattt aaaaaattca aaattattac
     1321 aaaaatcaaa tattaagaat ctttccttat ccatatcaac acctaataaa tctggacata
     1381 atctagttcc tctacctatc atttgccaaa acttagtctt acttctcact ttcttgaaaa
     1441 aaacaagatt tagtatttca ggtatgtcaa ttcctgtatc aagcatatct actgaaactg
     1501 ctatttgagg taatttattc ttatcagaaa aatcatctat tatagaatct acataattta
     1561 tactatagtc aactgctttt acaaagttcc caccatactt tggatacagt ttattaaatc
     1621 tttcaactat tgccctagca tgctttgtat tctttgcaaa aattatagtc ttaccaatct
     1681 tttcttctcc ttctattttc aacccctctg tcattagctt atttaaaact aaatctattg
     1741 tgtcagaatt aaatagccac tcatttactg cattattccc tatttcgtct ccaatactct
     1801 catcatcatt aaatgtattt tcatattctt ccttctcttc atcagaaagc tcatcatact
     1861 taattccatc ttccataatc ttagtcttaa actcaatact agtataatct actaaatatc
     1921 catcctcgac tgccttatta tactcataag catatgtagg tactccattt tccatgtcaa
     1981 aaactgaata agtattttta tctatctcat cctttggtgt agcagtaaga cccattaagt
     2041 aagaatcaaa ataatcaaag attgacttat actttttata aatacttcta tgcgattcat
     2101 ctattataat taaatcaaaa tgaccaggtg taaacaatct ctttccatcc ttagacttcg
     2161 tatcatcaat tgcattcatc attgttggat atgtagaaaa tatcatccta gcatcttctg
     2221 ggctatcttt gctatccaat aaattacata acgccaagtt aggtaatagc ttagtaaaac
     2281 tttttttagc ttgctttacc aatgcttttc tatctgctag aaataatata tttttaatcc
     2341 aattatgtct tgaaagtaca tcaactatag atatagcaat tcttgttttt cctgtaccag
     2401 tagcacatac caaaagagtt ttgcgttgct tattttcaag agcctcacat acagccctaa
     2461 tagcttcttt ttgataatat ctattggata tttcatcatt tattgttaca gaagttaaag
     2521 acttcttact ctttcttcta tctatcataa gttgtaattc tgatttttta taaaatccat
     2581 aaactttcct atctgaatag tcatcccaaa tatacatatc aaaaccatta gtcaaaaata
     2641 ttaccggtct ttgactatat ctcttttcca aacaatctgc atataattta gcttgttgtt
     2701 gaccaatttt cgggtcttta ctcgttcttt tcgcctcaac tactgctaat ggcttaccat
     2761 tttcaccata aagaacataa tctacatatc ctacaccact ttcattaggc ataccttgta
     2821 cttctatttc ttcccatata tcttttccaa attcccaacc tgcaagcttt aattctatat
     2881 ttatatatct ttttcttgtc tcaaattcgc ttacttcgtc tatcttgaaa tcatagtttg
     2941 cagtattaac ttttctcttt tcagttagtg actttcttaa ttcctcattt tcttttatta
     3001 cttcttcaag ctttttatct tttgaactta atctctcata aagattttta agttcgtctg
     3061 gtctcgtcct tttctcttcc ccatgaagta ataaatcttc attaaagtct tctgctgtat
     3121 attcatctga ataacaatag tctatccaac taacaaattg atgcaaattg ttaagtgaaa
     3181 gtattgcttc tcctctctct atgttcgcac tagtatgtac agataaatta cctaatttaa
     3241 ctatatattt taataaaggt aacatatcat agtctataag ttctataaaa ctattattat
     3301 gaatcaacgt actaagatta tcttgataag gcaataataa atcactatca ttagtatata
     3361 gccattttac tgcaagctct aaagctcttc ttgttatagt tgcacaagta gatgggctaa
     3421 ctagtatact tttttctgct tctaagcaag cagtattaaa actattgaaa tttttcttct
     3481 tttgtaaaaa atcaaaatta cccaaggctt ttctccccct tttgtccctt agttaaaata
     3541 aatctctcta aaaactattt cttacagctg tttcttatac taacaaatac ttcttcattt
     3601 tctgtaaaca attcgtacat atcttcaaac tgtggtatat catatccatg agaatcatca
     3661 tttctcaatt ttctaagtgt ttctataact atagaagaat ttttaggtaa taaattgtga
     3721 tactcacaag ttaaaaggtg gtcataaaag tttttagatt ttatatttaa atcaattgat
     3781 ttataataca acattgcttt tgttaattct agaaatgata ccatatagct tttaaagcct
     3841 tcttttatgt atctagccca taactcaggc atttcttcct tatcatataa gattttgaag
     3901 ttttgcaggt catacaagca agaaccaaaa tccctattta cttcttctat tgagttccaa
     3961 gataatttgt ttctaattac catacatgtc cctccaatct aagatatcta ttacatcact
     4021 gttttcaaaa tagaatttat ttatataatc cttaaaatct ttagattcat aaataacttc
     4081 accttctaaa atagcttttt taagtgctat tgtataatta tctgtaacaa tttttaggtc
     4141 tacatcaatg tctaatatat ttcttagagc ttttgtctta gttgcaagtt ctgtaattgg
     4201 tacttttttg ataggaatcc aagctatgtc tatatcactt tctgatgtat gtttatctgt
     4261 tccataacta ccaaatatta tgattgctag agtattttct tttggaaata catttattat
     4321 tgagttttta atctcatttg gtatatttct catttaatca cctcttgttt attaggtaat
     4381 attgcttaat tgaatttata tccataatct tttaatctat ttatacatat ttctctttct
     4441 tcatcagtaa acaactcttt atacttttct tttaatacta atacttctaa tgataaatct
     4501 aatctcttac actcccatag ccttgtaaat ccttcagtag ctccttcttt attgattaat
     4561 tctttagcag ctaataaggc tcctttgtta tttatcagtt gtaaaaatct tgttggctta
     4621 tagttacatt gtaaatcagc ttctttatag atatttacca tgtcatgata taaatctagc
     4681 tctatttgat tcatagattt tattccccca ttttaatact tgtattttag ttgaataact
     4741 ctcctttaaa agctttttgc attaatgagt taaagttgtt ttctagttct tttaagctat
     4801 cttccattct tgatttcaat ttgtctattt gtctgacaaa gtccacaaac ttattttgaa
     4861 gatatacagg tactttaggt atttcaactt catttaattt gcctactgta agacctggtt
     4921 gtgctgctcc agttgataat cgatttaaat ctaactcttt aagtgtaaaa tatagccagt
     4981 aactatttat atcaacttta ggttgtacta caactgcatg ttctgtagca taaaactttc
     5041 catttacata ttttacatta ccacataatg ctccttgcct acctatgaga ttaaatgttc
     5101 ccttatgtga atacattttt acatatcccc ttaaaccatt tcctccataa caaggatata
     5161 attcatgtga atttttttca taaatatctt ttgcttttat attttttcca gcttttaagt
     5221 ttgatatctc tcctaataac tctgtatccc aatctttact attagtaatc acatttccaa
     5281 acatctcaat aaatcttgat ttaactaatt catccaatgc ttctatttgc tcttttcttt
     5341 tatttattaa ctcttgagct ttatttaaaa cttttaccat tttattttgt atacaaattg
     5401 tgaaatatgg tatttttata tcatttaact tgcttttatt taaagttgca cccataactg
     5461 ctctatcagt attattaaaa tttaaagttc tcaaagcata atataaatat tcagatgtta
     5521 taagttcatt attttttatt tgaaagttag caattgcttc atttgtaaac atgtcttctt
     5581 ttaatatggc aactctacct atagacaact taaaactcat tacaacggta tttttaggta
     5641 ctaatttcat attagctttt tcaacagcta aatctgttat ctcttctttt gattttgata
     5701 tatatttttc tttcaaatca gaaattgata accatctatt accacttccc caataatcag
     5761 aagtatttct agatggtgtt ttgccaatat ttatatagca caattcattt aattttatat
     5821 actccatacc ctatacctct aacattcttt ctaattcttc taatccaaca gctatttcct
     5881 tctctaactc cctaactcta cctataatca cactaggagc atcataaact acttcctcat
     5941 acactacctc tttatactta ttaatagata aatcataccc attctctctt atctcttcca
     6001 catcaacaaa aaagctttgc tctgttcttt ttctatctac ttcaccatca agattatcaa
     6061 atctcttaat aatatcctct atatcactat cctttatctc atttctctta tcatccaaag
     6121 aataaccatc agctttcata tcatagaacc ataccttatc agttccacct gttcctgttt
     6181 tagtaaatat cattatagct gtagatactc ctgcataagg cttaaacact ccacttggca
     6241 ttgatattat agcttccaat ttatgattat caactatttc ttttcttatc gatttatgtc
     6301 cacctgttga accaaacaat actccatctg gcactataca tgcgcatctt ccaccaattt
     6361 ttaaaactct taaaaaaagt gccaagaata ataactctgt tttctttgtc ttagtcactt
     6421 ttaataaatc tgctgacact gcttcataat ctaaacttcc cttaaaagga ggatttgcta
     6481 acaccaatgt aaacttatct ttatctgtat taacctccga taaagaatcc ttatactcta
     6541 tattaggatt gtcaacgcca tgcaacatca tattcatagc acctattcta agcatagttc
     6601 tatccatatc aaatccatag aacataccat tattaaaatg ctccttcagc ccttgcacta
     6661 agaaaagact tgaatgatta tcccttaagt attgttgtga tgatactaaa aaacctgctg
     6721 aacctgctgc cgggtctact ataatatctt ctggtgttgg tttaataagt cttactatca
     6781 tatctattat gtgtcttgga gtcctaaatt gaccattagt acctgctgta gccactttgg
     6841 ataataaata ctcatacaaa tctccctttg tatctctatc cttacttaat tctaaattac
     6901 taataccatc aactatttta gataacataa gaggtgtagg aattttaaat attgcatctc
     6961 ccatgtactt tgcataagct gagtctttat ttccatgtaa tttttttatg aaaggaaaca
     7021 cttcattttg tactatttca tacatttttt ctgaatttcc ttcattagaa aacttactcc
     7081 atcttaaatg ttgatgttca tcatcaaata tcctttcaaa agaaactccc aaaagagttg
     7141 cttctgcttc attttttgtt tctacttcat ccaatccttt aataaataaa agatatgtaa
     7201 attgttctat aacttctaat ggattagtta atccaccagt ccaaaatgtc tcccaaatct
     7261 tgtctacttt gctccttaat tctcctgtaa tcatatatga ctttccctcc agtgttcata
     7321 aaataattta aatatatatt ttagtcgtac gccttaataa ttagtgtctt atattaaatt
     7381 caaaatttta ctacacagtt attaaattat ttttcctatt tacatgtttt agttaaaaaa
     7441 ctaatgcaat actcttattt tatgattatt atacatccca tacaaaatta ttttatcata
     7501 tatccagcat agaagtcatc tacctggata actcttacca tattataact ttttaatatt
     7561 aatttaaact tagaaagaaa ttttttattt aattatatca tcttccttat ttcaaatttc
     7621 tcaattacaa tctcaatcaa aaaataaaat aagatgctgt ctcaaaatag atataatctt
     7681 cttttttatt ttcggaatga tgaaaatata tttaatttca tacaaaaaag agtgcctcct
     7741 gaactaaaaa gttcattttg agacacccct taatttaatc ttacttattc tctcccttta
     7801 ttattaacac ttcactatct ctattgcttt tcctacacat ccacaaactt ccatcctact
     7861 cttaacaaaa tctttaactg actctaaacc ttttttacca tatttcttag ggtcaattac
     7921 ctctgggtca gaattaagca catccttaac cccattgcta taagcaatcc ttaattcagt
     7981 tgcataatta accttgcaaa tccctctttt tatagattcc ttaataactt catctggtat
     8041 tccagaccca ccatgtaata ctaatggaac tgatacaaca tctctaattt cagataatct
     8101 atctaaatca agctttggct ctcccttata taatccatgt gccgtaccta ttgctactgc
     8161 taaagatgat acccctgttc tctctacaaa ctcttttgct tctaatgggt ctgtatatgc
     8221 tcccccatca ccaccatcta aatcgtcttc cttacctcca actttaccta attcagcttc
     8281 aacaggtata tctgaaggca tacatgcatc tacaacagat ttagatattg aaatattatc
     8341 ttcataactt tcatgagaac catcaatcat aatagaagta tatccaactc taagtgcctg
     8401 catagctagt ccaaagcttg aaccatggtc taagtgcatt gctactggaa cacttgcctt
     8461 ctctgatgct acctttacat tcgctaaata ataatctagt cctgcatact ttattgttga
     8521 aggtgttgtt tgcaatatta ctggtgattt aagttcttca gcagcctcta ttactgccat
     8581 aaccatctcc atattttcaa cattaaatgc acctactgca taatgccctt cttgtgcatc
     8641 taataatatt tgtttagttg tcactaatgc cataatttct cctcctatat gtttatactg
     8701 atttgatttc ttcgttattt aattttgata ctatttctct acttgttttg ctatttaata
     8761 actcatttaa aaattcttca ttcattaatt ttgaagatag ttgagataat gtttttaagt
     8821 gtaaattatc accattatct gtagctgcta tcataaatat aatatgtgct aagcttccat
     8881 cttctgattc aaagtcaaac ccttcttttg atacacctac tgctacacgt ggtaccttta
     8941 ctgcatctgt ttttgcatgt ggtatcgcta tccccatacc aaaacctgta gatgatattt
     9001 cttctctttt aaggatttct tctttaaact tctttttatc taaaagaaca ccagatttat
     9061 caagtattcc aatcatttca tcaattacat ctgttttatt ttttccctta acatctaaaa
     9121 ttactgtttc ttcgctatat aaagagatat tattagatac gttttccgat tctgcacttt
     9181 catttttatg ttcatctttt gttacatttg tattttctat agtatttttc tcatttacta
     9241 ctttaggtgt aaccatcata taaagtacag cacctactaa tgaacctaca aatatacacc
     9301 ctacccataa aagtggctta ttgactaatc ctagaaccaa aaaccctcca tgtggagctg
     9361 gtacttctat tttcattata taagttaaaa ttgatgatat tgatgatgca atcataagta
     9421 ctggcaacac ttttaatgga ttttttgctg caaaaggtat tgccccctct gttatatgag
     9481 tactacctag tatataattg attaatcctg ctgctctatc ttcttctgta aatctatttt
     9541 taaataaagt agatgctatt gctattacca atggtggtgt tatacaagct gcagatacac
     9601 ctgccataaa taaataattt ccctgtccta gaagcatagt tccagttaca tatgctgctt
     9661 tatttactgg acctcccata tcaaatgctg acatacatcc tattactatt cccaaaatta
     9721 tagggcttga attttgtaac cccgatagaa agttcatcat agcattgtta agtgctgaac
     9781 atggcccacc aagtaacaac attgctacac ccacaagacc tacgctgaca ataggaatta
     9841 taaaaatcga tttaagacct tctaattgtc gtggtaattt tcttaacaaa tgtacaaata
     9901 gtttaactaa gtaacctgct aaaaatcctg atatgattcc acctaaaaac cccgaacctg
     9961 tatcaaatga tatcattcct ccaacaagtc ctgccaacat accagctctt cccgatatag
    10021 attgagctat aaatgctgcc aatacaggaa ccatcaagtt catagaatat ccaccaacag
    10081 attttaatgt agctgctatt tgattatatt gttctgaatt tgggtcaaat gaataaatcc
    10141 cccataaaaa tgatatagca gtaagtactc cacctgctac tactaaaggt aacatgtgag
    10201 atacaccatt cattaagtct ttatacaact tttgaccaaa cgataaattt ccttcttctg
    10261 caattttact gcttgataag tttccttttc taatagctac ttttccatca atacatcttt
    10321 gtataagttc attagcctta tgtatacctt ctttgacagg tacttctatt actggctttc
    10381 cattgaatct gtccatatca acatttttat cacaagcaac tattattgca tctgccattt
    10441 cgatatcttt tgctgttaac ttattttcta cacctatagc tccattcgtt tcaactttta
    10501 tgtcacagcc taattccctg gctgaatttt ttaatgcttc ttctgccata aaagtatgag
    10561 ctattccagt tggacaacct gttaccccta gaatcatttt ctttcccatt tttataccac
    10621 ctttctgact cttatatttt ttatataatc ttccacattc tccaaattac ctataccatt
    10681 actttcagcc acacttgctc ctgttgctgc tgaccttttt aatgcttctt caatatgttc
    10741 tggattttcc aaccatatac ttaaaaaagc agctaatgca gaatcaccag cacatgcaga
    10801 gctgactact gtaactggtt gagcactagc ataataaatt gattttccat tataaaaata
    10861 agaacctttt tctcctaaag ttagcaatat attttgagca ccttttaaat gcagtaattt
    10921 aagtacatcc ttaatatctt cttcatctct tacaataatt ccaaatatat ctttgatttc
    10981 ttcatcattt ggttttatca aatatggatt atactgtagt aactccttta attttggtga
    11041 acttatatcg agtattgtct ttatactttt atttttacaa attttaaata tatcctcata
    11101 gtaattatca tcaataccag gaggtagact tccacttatt gataagtaac ttatatctgc
    11161 taatgactgt atcttatcta gcatatctag tttttgtttt tcatttacat atgacccaga
    11221 gtttacaaat ttaaattcat cacttccatc atttaaaaat atatttatac gagttgtatc
    11281 ttctacccaa gtaggcaata cgtcaaatcc tctcttttcg ctttcttcaa ctatatattt
    11341 tcctgaaaat cctccaaaaa atcctaatat cttagattct ataccataat gacctagtac
    11401 aaaagttaca tttaatccct ttccattagg agaatataca gcatttgatg tacgatttac
    11461 taattttctc tttatcccat tagtagatat attcatatct attgcaggat tcgtagttaa
    11521 tgtatatatc attgtcttcc cccttgctcg tgctgtgtat gttttcatta taataaaaaa
    11581 gtctatgaaa atcttttcac aaatagaaaa tcgattttca tagacttttc aatttaattt
    11641 tcagaattat tatatctgac catataatct ataattatcc tagatataat atacaagggc
    11701 aatctggaag atacttcata ttcagaaatt gatatatgtt catgtttgta tcctacaata
    11761 tataaatccg aatactttat aagttttgca ctttcactac aacagcaagt tataacctta
    11821 gctccaacta aattggcatt ctgtgctgat tctacaagct ctatggtatt tccattcaat
    11881 gaaaatataa ttataagttc gtcttttttt attgatttag attttatttt cattatgttg
    11941 gggtcattta taaaaacaac attaaaatca agtaattgca atttaagact aaactctttt
    12001 gctacatatt cagtaagccc tcttgccaaa atatatatct tactagattt atttatagtt
    12061 tttattatat tgattagtgt agttaaagat atttgttcta taactctctg tgcttctata
    12121 aaggatttat ttattatatc ccccatattt tgtatatcat cattttcatc ttttactgtt
    12181 aatctatacc taagttcatt gaatccattt aaaccacatt ttctaattgc tcttgaaacg
    12241 gttgctggag atgaatatgt ttcatgagca atctctacta tagataattc tggtaatatt
    12301 gattcatttt catttataaa tttaattatt tcaagttccg tcttcgttag attttcagaa
    12361 ttacttttgt ttagctcaat tatcatatgt tatctcccct tatataagtt ttatttgtct
    12421 aaatttatcg agtaatataa tatattacct cttaactact tatttttata tacaattaag
    12481 atttagtcaa tgtctatata tgtaaattta taatataata attttttgct taaagtttat
    12541 tatgttatct tatagcattt taaaataaaa aagaattgta cctctaccaa aatttggatg
    12601 aagtacaata gatattcctc atactgatat gtttatgatt aaaagtataa atataaattt
    12661 cgctatgaat catataatta ataaatcatt ttatttacaa ttataacccc ccttataaaa
    12721 ctatattaat aatatataca gtatttataa ggggggttgt ttatatttaa aaactattta
    12781 cttttctctt aattcttctc cattagttct tattacattt tgataccaat aaaaactatc
    12841 ttttttataa cgttctttac ttccatttcc attgttatct atatccacat atataaatcc
    12901 atatcttttt tccatttctt gagatgaaca acttactata tctattggcg accacataca
    12961 ataagctaat atttcggttc catcagctat agcttcacct atttgttcta tatgttttcc
    13021 taaataatct attctgtaac tatcatgaac ttttccatct tcaaatttat catgcattcc
    13081 aaatccattt tcagctatca ttattggctt ttggtatcta tcataatatt gagataaagt
    13141 attgtataat ccttttgggt caattgccca cccccaagga tttgctttta aatatggatt
    13201 ctttatattg tcagttggat tcatggtgtt tttacttgct gatactgttg tagaataata
    13261 gtatgataaa gctagaaaat ccatagtatt ttcttttaag attttatcat caccttcctc
    13321 cataactata tttatgttat tttctttgaa aaatctctta atatagactg gatactctcc
    13381 cctaaaactt acatcagtga aaaaatattg tagcctattt ctttttaaag caaatactac
    13441 attttcaggg tcacaatctt ctggataaga tgtacaatct gccatcatca tacctatcat
    13501 catatctgaa tttatttctt ttccaagctt cttagtcaaa gctgtagcta tgaattgatg
    13561 atgaatagct tgatatctag tctcttctac attatcaact tggtctacac aaaatgcaac
    13621 tgaattaaat gattcataat gtattaaatt tatttgattt ataactatcc aatatttaac
    13681 tttattttta tatctattta gtattaattc tgcatatcta acaaagaaat ctaccacttt
    13741 tctattagac catcctttat attttaatac taaattcaaa ggagtctcat agtgaagtag
    13801 tgttacaata ggttccatac cattatctat tacttcattt ataagattat cataaaactt
    13861 taatccctct tcattaggct ctaaatcatc tccatttgga aatattctag cccaatttat
    13921 tgaaaatcta aaacacttta accctagctc tttcaaaagc tttaaatctt ctttgtatgt
    13981 atgataaaag tctattccac tacgcttagg ataatatcca ttattatctt tcatagcaaa
    14041 ctttattctt tctatagtca tatcagactc ataaggttta gatatatctc tatctttatc
    14101 atacatatgt acatcagcta aacaccaacc tttgccacct tcattccatg caccttctaa
    14161 ttgattagct gctacagctc ctccccataa aaaaccttta ggaaacttac ccaatttaat
    14221 cactccttat tttatttatt taaatcattt actattattt accttatatt ctatagactt
    14281 tactattcct actaaagaca tagttatatt taactcgctg aatatggtca taagagtatc
    14341 ttgagcatgt gcaaataaca gcgaatgtgc tcttttctct ccaccagctt ccccttgtat
    14401 aatcttggtt tgagctctat gtgcttctgt cataaaccct tttgctttag ctagttttaa
    14461 atcagcattt ctataatcaa acttttccaa atcattaaga gcatgtttaa tttctgttct
    14521 agcctcacct gcacgaagta ttatttgcat tgctacttct actaattctt catctattat
    14581 atgttcttca attgtcatat ttataatttc ctttcaattt ctaaatttaa acctcttgac
    14641 tctctaattt tatctcttct ttctcataag ctttaaaaaa tggataccat atcataaata
    14701 gcaatactat atttattgct aaaacaataa gacctctaac atcttgattt accataaaag
    14761 tagatatacc aaaaggaaca taccataaat taaacacctt acttggaatt gtaacaaatc
    14821 ccatactcat tgataaataa gtagttattg atattactac tgttcctatc cacataggta
    14881 acattaaaaa tgggttccat gccactggag ctccaaatat tactggttca tttatattca
    14941 ttatagaagg gacaatacat gctcttccta ttgcttttat tcttccaact ctggataaac
    15001 acatcattat aactaatggt aatgtagcac ccaaccctcc taaagttatc cagcctgttt
    15061 gaataacttc gtaaacatat atattagttg ctactcctcc tgatgctact gctgctgcat
    15121 tagctgcaat tgcaccaagt tgaattgaat aagttatagg agatagtacc cacccactta
    15181 ttcctaaaga atatataaat actactaaga agtttaagag tacaaatcca ggtaatgtct
    15241 gtgctatatc tgatactggt ttaaatactc taactattat ttcaaacatg tcaaagttga
    15301 gtccataaat taatccccag cctacaaaca ttatcactgt tataggtaac atattatcaa
    15361 accacttgct tacaaaatcc ggaagtgctg aatcttctcc gaaaaatgaa aaagaaccaa
    15421 ataaagacat tattaatcca gagaataatc ctgcaactag cgatacaagc attccttctc
    15481 ctccaaacct gttgaagtta aatatagctc cttcattcgt aatctctgga cgaatcatca
    15541 ttaacattaa tccagcacct gcaagaccag ctactacact catactttca aatttcttat
    15601 tcttcattgt ttccataggc aataaaaatg ctataaataa tcctaaaagt ccaaatgtat
    15661 attgatttat tggacttaaa tcaggtaaaa agcttatgta attcttcaag acagaaacta
    15721 tagttactaa tgagcctaca aataccattg gcaaaacttt cattatagtt gattgaactg
    15781 attgtaccca tatattctta gtcatattat ttacaacagg agtaaatttt tcacttaaaa
    15841 aatctataaa tttattcata aacactctcc ccataaatat tttatttgtt tgatattagt
    15901 tctagtgctt cttccaaagc ctttgcacca tctagtcttc catatgcagt tttagatata
    15961 catgctactg gaacgttata ttcattagcc ttttgttgaa tttcttcctt catataatct
    16021 aaatgtggtc ctagtaataa acaatctatt tctgataaat acttatctat ctctgactca
    16081 cttctcgctt ttatatctgc ttctatacct tttttctttg cactctttct catactagtt
    16141 gccataaaac ctgtacttgc tcctgaacca cacgccaaaa gcactcttat atttttcata
    16201 ataatacctc caatattttt atccattaat ttatttctta atgaactttt tattttatta
    16261 tgtttatatt ttacttccca aataagttca tttcacataa atatatttcc taataatagg
    16321 aaatcattta ctcttaatta gacctattaa tatagtataa tgtatcttat aatacgtaca
    16381 ttcaattata taattatcat atcatacaga ttttatttta aaactatgcg agggaatcta
    16441 atgaaaacat ctaaaaaaga gctattttta aatttcataa aaacgcaaca tgattggata
    16501 gattcatcca cattagctaa ttaccttaat gtatcaacac gttcgattag aaaatatgta
    16561 aacgagatta attctaatgg tgcatttata ttatcttcta aaaagggcta taaagtaaat
    16621 ctcaatacta attgtcaaac taagattgat agttcagaga atatatcacc tgataacaga
    16681 ttaaatttaa ttttaaaaga acttatagtt aatagtaatg gaattaatat atttgattta
    16741 agtgaagaat tatttgttag cccagctact attgaagggg atatagtcaa agctaataaa
    16801 tttataggaa gttataattt aaagataaag caatcgaaat ttttattgaa actagttgga
    16861 aatgagtctg caaagcgcaa gttaatgagc tctataatat ttaaagaaac gggaagtgat
    16921 tttttaagct tatttgatgt acaaaaaata tatcaagagt ataatttgac taaactaaaa
    16981 gaaaatataa tatatatatt aaaaaaatac aatttattta taaatgagta tgctataaac
    17041 aacatacttc ttcatttaat gataactata gacagaataa aaaagaataa ttacatagac
    17101 tctgttgaag tagtaaacta catagacaat aacgtagata taaatattgc aaaagacata
    17161 tcaaattttt tagaatctga atacaatata actttaacta gtgcggaatt atactattta
    17221 gtatttcagt taacaaacaa aactacagtc ttaaactaca atcaaatgga tactaaatca
    17281 ttgtcaaatt atatagatga acactttgtt aaattaacta aaaaaataat taaaaatgta
    17341 tatgacttat atcttataga tttatctgat gaggaatttg ttgtaaaatt tactcttcat
    17401 gtaaaaaatt taatcagtag agccaaaaat aatcaagttt taagaaatca aatacctcaa
    17461 aagttaaaag attcctaccc tttgatatat gatatatctg tttatatttg caatcagatt
    17521 caaactttag aaaatgtaga tatagacgaa gatgaaatat cttatatatc tcttcatgta
    17581 ggttcattct ttgatagaca aaaattatta gaagataaag ttctatgtgc attaattact
    17641 ccaaattact atgatttaca attcaaaata gtaagagacc ttgagaagag atttaatgaa
    17701 tccatagaaa ttattcagat tttttcagat actcataatc ttgattttga caataaggta
    17761 gacatggtta ttactacttt accaattaac gatagatgcc ctataccttt tgtttatgta
    17821 aatccttacc taaatagaaa ggattatgat aatattcaaa gtaaatttat tcagattaaa
    17881 gatagaaaaa atatacttac tgtacaaaat catttagaaa tgtactttag tgaatctttg
    17941 tttatgaaaa atatatattt agatagtgct aaagattata taagatttat gggaaatatt
    18001 ctgtacgaaa ataaatatgt caaacccaat tatatagatg atgttcttat aagagaaaaa
    18061 atgtcttcaa ctgcatttaa taacaatgta gcaattcctc atagcatgaa aatggatgct
    18121 ttaaaaaccg gagtatgttt aattgttaac gataaaccag ttaaatgggg tgaggaaaaa
    18181 gttcaaatta tagctatgat acctataaat gaaaaagaaa aagaaaaatt caattacata
    18241 tttgaaagct ttattgaaat attatctgaa tggaataatg ttaaagaact tactaaggca
    18301 gataactata gtagttttat gaatagaata gcttatctta ttcaaaatat ctaaatccat
    18361 taatttattt ccaatatatg tttcttaagt tattaacata aaatagataa gctgtcacac
    18421 taaaaagtct aaattaatat gtgacagctt tttacctaat ataccttgtt ttaatactac
    18481 atattttttg actgttgtat actagttata agattgaata tttgtgttgt tgtagctgtc
    18541 atattctcac tagcagttgc tttatccatt gcttcttcta ttgtcatagc tctatttact
    18601 atagaaaaat aagcatctat accctcttca ttacatttaa ctgcatcttc tgttgtggct
    18661 ccagccaaac ctattacttt taccccatat ttttttgcca gttttgcaac tcctattgga
    18721 gccttgccca tagcagtttg attgtccaat ctaccttcac cagttatgac aaaatctgca
    18781 tcttttaact cttcctctaa tttaatttct tctaatatta tttttattcc agattctaat
    18841 tttgaatcta aaaatgctaa aaaagcaaaa cctaatcctc ctgctgctcc tgctccttct
    18901 acattagcta catctttact taagtaattt ttaactactt tggaaaaatt tctaagccca
    18961 ttatctaact cctcaattat ttctgatgtt gcaccttttt gagacccgta tatgtatgca
    19021 gctccatttt tcccaaacag cggattattt acatcacagg ctattttaaa cttacattca
    19081 tctaattcct ttaatctatt ttctattttt atatgtctta tttcatttaa aacttttcca
    19141 cctaatccta ctaacttgtt atttttatca taaaactcaa accctaaggc ttgaagcata
    19201 cctatcccag catcattagt agcacttcca cctatcccaa ctataaaatt tctatagcct
    19261 ttatttattg cttcctttat tatttcccca acaccataag ttgtagtgta taaaggattt
    19321 ctaagttcag tcggaaccaa tgttaatcct gatgcttgag ctatttctat tattgctgta
    19381 cttgtatttt ttaataatcc atatgttgca tccacttttt tagctattgg tccagtaact
    19441 tctatagtct cttttttacc attcatccct tgaactaaag catctactgt cccttcacca
    19501 ccatcagcaa gtggcaatat ctttacttgc gcatcttctt taaccttcaa tatccccttt
    19561 tttattgcat ttcctgcctc tattgaagat aagcttcctt tcaaagaatc tatagaaatt
    19621 aacattttca tacttatcta ctccttttta ttactcctta ttttaaacat ataattttat
    19681 aagcttaatt tatcttttat tttatactct acatctctct ttgtatacaa cttataccat
    19741 taatattctt ttatcttata aaaagggagt gtctcaaaat aaatttctta gttcatgaga
    19801 cactcttttg tataaaatca aatatatgtt tattatttca acaatgataa ggaggattat
    19861 ctctatttta agataattcc tttttagcac tatctaatca tttttagcac tatttaacca
    19921 gtatagtttt aacttggttc attaagcact ctacatttac agagttatta tctattgata
    19981 tagattctaa ttctttttca tttaggttaa tccatttagc tgttttaata tttttgttaa
    20041 atactatgtt agctgatttt gtttcttctt tagaattata aaatctaact actaatgcct
    20101 cttctttttc agcctttttt acaacactta acacaaggtc cttattagtt tccttaagta
    20161 aactatatga ataatctact tctatttctg attgattcaa tttcatagca ttatatggca
    20221 ttttattata agtttgtatt tttgttaaat actctttagc catatttgca acatttgctt
    20281 ctagtgtaga tttttcatga gtaactattg ctaagtctat ttttatatct cctatcattt
    20341 gagaatctgg agtaggcaac tttattcctg atggacgacc tgggcgtctt gccatttctt
    20401 ctttacctag gaatcctaca cttctaaata atgttatagc tattgtgtca aatttttccc
    20461 ctactatttc aaactctctt gtactgtttg taagaacagc tactccatgc tctttgtcag
    20521 acaatccaac gaatgttagc attggatata tagaatctgg tctttcactc cagtcttcct
    20581 gttgccaaac ttccatagca tcatcataca cacttctctt tatatgtcca aactgattat
    20641 cagacacaga aaactttgaa gctatatttg ttggtatata agttctaacc ctacaatctc
    20701 ttgcttggtt atttatgttg aatactacat ctattatagc tttatcattt ggaacattta
    20761 taactatatc aaagtcaata aatgaatctg ctctattatt ttttctagct tgtatatctt
    20821 ttggtacttt taatctataa tttatcttta tctcattatt aaatctattt ttctttatag
    20881 tggaatttgc tactatatta tcgttaaata ttaatttctc atttggaagt ggtgaaaaat
    20941 catactcatc tccttcatct cctccatttt ccatcaataa tacattttca aaagttttat
    21001 ttaatttttt atcaaatatc tttaatgtcc cgttttcatt aacttgaata gtataaaagt
    21061 cattatctac catgtcacat acttcataag ctttttctaa catatgttca acttctttta
    21121 tcatatacgc cttatacccc attgcaggta ttttatcttt aaagtttatt gtatatacca
    21181 taaatgaatc atagtttcca taatgaacaa tttgacggtc tattaatcct gcatctatta
    21241 cttctttatg taccacttca aaatccaact tattattatc tttatcaaat atttcaaatg
    21301 ctttcatttt agttataact tgggcgctca ctatttcatt cctatcgtaa ggcattaagt
    21361 taaatgctat taacttatct aaagccattt cacaactcat tgcatcaact attcttcttt
    21421 tgtaaaatgt tattaattgg tctacttttt cctcagctaa gaaaaatcta ttcattattt
    21481 cctggtgaac tttatctgaa caacaacatc caatgctgtc atgagcatga tttttcatta
    21541 tttctttcca aattaactct attaatccat gatgatattc aaatcctaga gaatatgcaa
    21601 tagaagctag tggctccaat atgttagtta tctttgattc tattctagca tttgctgatt
    21661 ttatatccat tcttgttgag aatatacttc tatgaaccct catatatttc ccatctaaaa
    21721 attccccttt tattgtatct atatcctcat ttttttctag ctctttaaat atgttttcat
    21781 atctacttaa gaaaaatttt ctgtctggat aacattcttt taatttatat ataacttcaa
    21841 atatattctt ttgaattggc atttggtcat gtccatttgg aagtattata tggtcagtag
    21901 ttgcaccctt atcaagaacc ggtaaatatt tatccattct tgttcttagc tcatcttcat
    21961 tcattggtag atattttcct attgcataac ctaatggaag tatttgaacc aaaacagaag
    22021 acccatcatc actcttccaa ttaaactctg ttttattagt tccttttctt tcagaacatc
    22081 ctctccaaaa cattgagtat tttatatcaa acccatttag tatttgtggc atttgagcac
    22141 tttgaccaaa tgaatctggt aaatatccta ttttcatata ttctccaaat tcatcacaat
    22201 cttttattcc atatagtaaa tttctaagta tggattctcc accaactacc atttcatcag
    22261 tttgagtata ccaaggacct attattagcc ttccttcttg aactaatttt tttattctct
    22321 ccttacattc aggcttaact gctaaataat cttctaatat agcagtttga ccatccatca
    22381 cataatatgg atagttatga ttattttcta acatatccat tatttcttcc atattattta
    22441 caagtaatat tctagattcc tcagttgaga agtaccattc tctatcccaa tgcatatgag
    22501 gaactatgtg tacatttttt ttcattgctt atcctccaaa acttatctga taatattttc
    22561 tgtctaatta aaaatccaat aaaaatacta ctgagtttac aaaatttttt aatatttatt
    22621 ttatcactat aattaaacct ttgatttaaa tatctaataa cgtatctttt aatgtatctt
    22681 tctttgattt ttttgtttcc tagttattat taataatatt gctgatatta tagctccaag
    22741 tactgccgca aagaaccata ttgatgctat aataaatcct gacatacttc cctctaaaag
    22801 cgccaatgag aatatccccg ctccaggcac ttgaagacct atattgaaga atgctactat
    22861 agacccagtt atggctgaac cagctaccaa tgatggtata acacggattg ggtcctctat
    22921 cataaatggt attgcacctt cagttattcc agccaatcct aatatccaag tttgctttcc
    22981 tatttccttt tctgattctg aaaataaatc tctgcctaaa acagtagcta ttgtaactcc
    23041 aaaagctgaa accatcttaa ctgatgcaaa tattgcataa ggtataaaat ttccacttgc
    23101 tattgctcct attgcaaatg tataggctgc tttatttatt ggtccaccta agtcaaatga
    23161 aaccatagct cctattattg cacctagtag tattgcattt actccagtca tcccatttaa
    23221 ccaatttata agaccattgt taagtgctgc tacaggcttt cctactacaa ataacatcaa
    23281 taaacctact accaaagaac caactactgg atatacccaa aaacttacga atccagctaa
    23341 tgtgcctttg gctggtatat tcttctttaa aaattttact atataaccag caagtaaacc
    23401 tcctaacata cctccaagaa atccactacc tattaaatca gctgctagac ctgctgcaaa
    23461 tcctggtcca agacctggtt tttcagctag tgagtaagac atatatgctg ataatactgg
    23521 aaccatcaat gtacctaaca tacctccaga aagctttctt aattgccata accaagaacc
    23581 ttcttgattg tatagttctt gtagaccaaa tgcttgagat gctaatactg caaatgcaag
    23641 tatcatacct cctgccacaa taagtggtat tatatgagat actcctgtaa gtattgctga
    23701 ttttacatct tttttccatg atgatttttc acaatatgat atatcagatg atttttctat
    23761 ataatcacct ttaccttgtt tttgtgctac ttctaaagct tcgtatatta tttcttttgc
    23821 tctttttagt ggagctgcta ctgacgtttt tatgtttggc aaatgttcaa atctttctgt
    23881 atttttaggt gctacatctg ttgcatatat tattgcatct gcccttttta ttatatctgg
    23941 cgtatgaggg tccaccatac cattagctcc ttgcttttct acatacacat ccacacctat
    24001 ttctttacca gctttaatta atgcttctgc tgacatatat gtatgtgcta taccggcagg
    24061 gcatgctgtt attgctaata taattttttt tgattcattt tgaatcctaa tctcttcttt
    24121 tatttgttct ttcttttctt ttatatctaa gttattaaat aactctttct tagtttttga
    24181 atttaacaac atattcatat aatcttcatt tgacagtcta gtaactaact cagaaagtat
    24241 ttgtatgtgt gtatttccac cttcactttc tggtactgct attaaaaata ttagctctac
    24301 tttattattt gggtctacgc tttcccaatt ttctattggt gtctctaagg tcatagctgc
    24361 aaatgcagct tctttaactg acttagattt tccatgagga attgcaagcc ctctttcaaa
    24421 acctgttgga gataaagttt ctctgtacat tacagcttct aaatattcct cataagatgt
    24481 taacttgcct tcatcatata gctttcttgc taagaacttt attgcgtctt cctttgtttt
    24541 tattttttgt tttaaaaaac ttactaaatt tctgattatc ttttcataag ttggtaaatg
    24601 ccgatatatc ccatattttc gggctttatt ggcattttcc ttttggaaga tacctcgcat
    24661 aagatggcat ttaccagcat gaaaatgcaa ccaaaagtag taaataagta gtaagataca
    24721 ttttcgatat taagaagcca gcctttctaa ttcagccttt gcagactgga aagtaccgtg
    24781 agcataatac cctaatgtca tggttatgtt tgcgtgcccc atgatgtatt gcagggtgtt
    24841 agggttcatt cccttatttg ccatgttcgt acagtaagta tgtctgaatg aatggggcgt
    24901 gatgtttggc aacttgtcct tatgcgtctt gttgtacttc ttaatcagcc cacgcaccat
    24961 actttcataa tttcctgcca cctttggcaa tccctcacgg ttcaagaaca agaagttgct
    25021 gtaaccgtca acaacaagcg gttgtgcttt tcctcggttc tttaaaattc gttcaagtgc
    25081 ctgatatgct ctttctgtca atggaagctc acgcttcccg ttttttgtct taggtgtgga
    25141 aatatagtaa cccacctctg tatcacgcaa tagctggtgg tctatgttaa tcacacggtt
    25201 aagcatatca atgtgtgttg tcagtccgca taattcagag atacgaagtc ccgtttccag
    25261 cagaagaata acttcatcac gatacttgct gtagacatta tctgtttcca taaaggcaag
    25321 gagtttttct tcctgctctg gtgttaggat aacctttgct tccgtatcat cttccagaac
    25381 atcactcaac ttgaaattga aaggattttt tctgatacag tcgtcttgta tcgccatata
    25441 gaatgaagcc tttaaggaac gcttatagtt atctatcgtt ttgtaggaat aacctttgtc
    25501 attcattctg atagcccatt ctttagcgtc ggactgcttg accgtatcaa tcgccctcat
    25561 acccaatggg tcattttcca gtgcgttcat aagatactgt cgtcctattt ttgtgtttct
    25621 cttgatgttc tttctttgat tgttcttctt ttcgtaaagc tggcagactg tcattttacc
    25681 acccactgtg tcgataccgt cgtcaaggtc tttttttatc tgcttttctt tttccctcaa
    25741 cgatatatca tcacgttttc cagcaggtgt tttgtctgtc ggtacaagtt tccaagcata
    25801 tacaaactgt ggcttcccga atatatcggt atatttataa acgtatcttc cgtcttttct
    25861 ctggctctct ccaagacgca aattgcgtcc tttactgtct tgtcttttca cttttgacat
    25921 ggtgtaaagc tcctttccgt catggaatga gccgtgatac gctactaata ttataccata
    25981 tctacggctc taagacatca gatttcgtcc agtgtatcaa taattttttc aaactgtttt
    26041 cgttttatct gaatacggtt tccattgacg ataacccagc cagagtccag attttcctcg
    26101 gcaagcctac gcaacttctt ttccccgata cgaaaatatc gggacgcttc ttctatacta
    26161 agggtatatt tttcccagat aggcacatca gtattgttca tgttgcaaca ctcctttctg
    26221 tatgtcttat cataagcaga cagacggctt cggaaagctc cgttagtatc tcaactattc
    26281 ccattcttgt gggcagaacc gcaccatgcg gacgtatcat tattctgtga tagcaggtca
    26341 tggcaaagcg accattccac aagttgctct cggatcactg cgtgggtcgc tcactttctt
    26401 gtcggaaagg tcatggcgta cagtccccgt ggctcgctgt atcacaaacg tatctgtctg
    26461 ctttattcag ttgtcaaaga acaatcggcg aaagccgtag gcttccatgt ataaaagcag
    26521 gagcagggca ggaaagaggg ggatttaacc aatcacgccc tgctggtatc tgcttattct
    26581 tcttcggttt ctatatctat atcaatctca aagtctaaaa tcattttgat taaggcttct
    26641 cgaatacgtc cctttaattc catatcaaca acaatataaa cattgccgta ttcatcatat
    26701 aaaggacgta aacaacactt tgatatgtac gggtcataga ataccagtaa tttctcaatc
    26761 gcagtttcat ccccgtccat agctgatgaa atcaaataat aagacgggtg cttgtacttt
    26821 ttcttcattt ctatttcttc tcctttagta tctctttcat agtttctagg gcacggtgtc
    26881 tgtgtttgaa cacagcccct ctggtataat tcataatatc tgcaatctct gtatcactca
    26941 tttccaagaa gtaatacatc aatacattgt tacgtttttt ttcgggtagc tgtttgagag
    27001 cttctgcaag ttcatcatca aggactttaa tatctatgcc acacacatta aaaattgtgt
    27061 agtttgtttc gtagtcgtcc catgttgcca gactttctac cgtaatgtct ggtaattcgc
    27121 taaaagatat ttccttgttt tttcttcgtt ttaattcttt acggtagttc cagataatac
    27181 ctttgacaac ttttttcagc ttacattcaa actgacactg aattgttttt tggaagtcag
    27241 acggtttcat aatctcacct cctttccagc tatgaattga aaagtgttta tccctctttc
    27301 cgcaccatat ctgtacagca aggtgtgatt tgcttccaaa aaaatgaaaa taattaaaaa
    27361 aacttttcgg gtattaaaaa agcccgcaca aaatataaat tttgtacgag ctactggtat
    27421 agcgtattta attgtaaata gtatgctgtt aaattgggct tgaatatact gaattgtaca
    27481 taaagcgttt catacggcaa gcccccttta caacatttaa accttaacct ttgtcttttt
    27541 ggtaatcgta taaatatatt cctctggtgt tgggcgctta tttccaaagt atcgtttaaa
    27601 atttgcctgc acgtctgggt ctgtactttc catagttttg tctatggtat cttgtagcat
    27661 agccctaaat tcagatacag aaacaccttg agctttagca ccaactttca taatataatt
    27721 catatttcgt cttttaagtc ctatcattag aaccaactcc ctaaaataag ttaatcttct
    27781 ttttcaaaaa ttaaatctat ctttcgcata cagccttttg cgtcaatttc tgtgggtaca
    27841 aaccctgcgt agtgataccc cgtttttgcg taactgtcaa taatttcttt gtgtttatca
    27901 gtacaaagga atacaactcc tttcgcatta tagttaattt ctacatactc atattttttc
    27961 atttttcaaa actcctttca aatacaaatt tatctttgga tttattgact tttacaaact
    28021 ggaattattc ttctattttc aaatcattac cctcaaaaat aaaattattg atgtcaatcg
    28081 gtaaacatat tagaacgcaa attatagtgt ttggttctaa agaatttact ttttttgtag
    28141 aattatagca ataaaattca gataatctcc accgccagct tctattgcct tacggtcatt
    28201 tattgcatat tcattatttt gctttatcca atccgaccaa acttccgaat tactttccat
    28261 ttcatatatt gaaattaaat ctgccttttt acaatgacta attatgttag accaatatgt
    28321 tatatcatgt atataatcaa gttcttctgg tgtccatgat aacaataact cttctggaag
    28381 tttctcatgg caatctttct tcatgccagg aactgtgata taaacaaacc cacctttctt
    28441 aacaaatgga agcaattttt catctaaata tatagggtca cgtccaaaat aattatatga
    28501 atctgtactt actacagcat caaaaaactc tttctcaaat ttaagttcat tggcgtcagc
    28561 ttttacagat ttaatttgtg cagaagataa tcccattttt ttgaaaaatt cttcattttc
    28621 ttttggctca ctccataagt caactgcaaa aacaacaaat ccatattctt ttgcaagaaa
    28681 tacagaagtt aaaccttgtc cacttccaag gtcacagaca tagctgccag acgggatttt
    28741 atgattttgt aatagttcct cttgtaattt tataggattc ggtcccataa tttttgtctg
    28801 taagtcgggt gtaaaatatt ttttactgcg aatatattcc attttctcat ctccattatt
    28861 gtaatatttt aatagttaat atttgtgact tataacaata ttcattttga gttcctctgc
    28921 tgaaattaaa aagttatgtt tgcattcagg acagaatatg attattttac tttttcaatt
    28981 gtaatatatt tcttgacaaa ttgattaaca tgtgtggtta attgagattt taaatcccaa
    29041 caattttcaa ctgcgtctgc tacacttatc aatagaaata atggcgcata atattcaata
    29101 gcaagctgtt ttgtatcaca ctcttgaaga acaccttttt taatcatttc attaaatata
    29161 tcttctatat aactgatagg accatttaca agacattttt gataaagcac attcattttt
    29221 aaatttctat attgctctag tattaacatt ttgcgaaaat tagacgcaaa ttcatcagtc
    29281 acccaaaaat cgtattgtgc aagagtaaat gttattagac ttccaatagt tgtatttctg
    29341 tatgcattag gggttacctc tattgtgctt tctggtactt gataatcctt agctcgctta
    29401 ttatcaattt gatacatacg ttctacgata cagtcaaaaa tatcttgctt atttttataa
    29461 tgtttatata atgctccttt tgtcattccc aaggcttccg caatcatact aacagaaaca
    29521 gcctcataac cattcactgc aaataagtgt agtgcaattt ttaatatatt ttcttttgtg
    29581 tcagacattt acaacctcct tctttagtaa acgaacgttt acttctgtaa accattatag
    29641 taaacgctcg tttacttgtc aagtagtata aaatgtagtg tgcctttggt tcataggttg
    29701 atctctcgat cgctggtgct ttatgccttg caccatattt cctacataca tttctctatg
    29761 caaaagactg ttgattgtaa agaattgtac tcaaagcttt ccttaacgtt agccccccct
    29821 taaagcactt tctagtatct aaaaacatca ttctacaaca taacagattt caataaacct
    29881 taatacatct tgtcgtcctc gctatctttt gaacgattgt tagaatttct cctttggtta
    29941 tacttcttca taattacaat tttaagtaag ccaacgacaa tcaaaaccac aacaatgata
    30001 atccaccagt attgttccat aacctacttc cctcctttcc ctttcaatag ccttaaatca
    30061 tcttccctgt cctatgcaga atagagaagt tcattcgtat taacatgtac catacaatgc
    30121 ttacattgtg gaaagttgtt ttcaatcgtt tgatggactc cctcagcaat tctatgagcg
    30181 tcataaagag ctaaattacc atcagctaat atttcaatat ctacatacat tttcgctcca
    30241 aacatgcggg tttttaattc atcaatacca tcaacccctt gctgctgaag cacaacttcc
    30301 ctaatctttg tttcagtagc attgtcacaa ctatggtcta ccattttgtt aatgctttct
    30361 ttgaaaatgt ccaaagcagc ttttaagata caaccgcaaa tcacaacgct tgcaataggg
    30421 tcgagaatag catagcctaa acgcgctcct aaaatgccaa ttaaggaacc aaccgacgag
    30481 agtgcatcag agcggtggtg ccatgcgtca gccataagtg cgtcagaatt tgtatgcttt
    30541 gcagcatttc ttgtaaacca gtacatccac tcttttataa caatggataa gactgccgcc
    30601 gtaagtgcga ttccactagg gacagaaagc gtattgtact gtcctgaaaa gattttcttg
    30661 atacctgaat atcctattcc agcacccgtt atagccaatg caacagagag aataatggaa
    30721 gccacacatt ccatacgttc atgcccataa ggatgttctc tatcaggctg tttttctgat
    30781 attttaattc caaccatcac aacgattgtg cttaatacat cagaagccga gtgtacagcg
    30841 tccgacagca tggcacttga ctttcctata tatccggcag cgaatttaag taacgataaa
    30901 cttaggttga taaatagtgt gattttggaa acctgaatag ctctatttac ttttccgttt
    30961 tgcagaagtc tacccttttc cataatatgc ctccttatcc tgccagcttc cgaaacagac
    31021 gttttgtaag gaagtagtac cagactaaag ctaccaccaa tcctatagag aaataaagaa
    31081 ccgcctcatt ctgcatggct ttcgctaccc aaaatgatgg aaggaagcca accgcgaact
    31141 ggtagtaact atcaataaaa aagggggctg gtattcccag caatgtaagt gcagctagtt
    31201 ttgtaactgc cataccctct aatttattgc ttgataatgt aataatcata agtgaaacaa
    31261 taattgcctg tactgagccg agaagcgcca aacatattgt cattccaatg gacaactttt
    31321 ctaatgaaaa aagtaacagt acaataaaag cgatgaccgc cgaaataatt gagggtactc
    31381 ccaaccttgt aaagagatac cccgacttac caagaggggt aatcgaaaag taccgtgaca
    31441 ctttatcgtc gatttcctct aatgtaatca ttgcaaatgc aaagcagagt aatacaggag
    31501 ccatgataga aagtagtaaa tcaaaaatcg gatagtatgc tcgcaaagaa aaatctgtta
    31561 ttctttcaag aaagggaata cctaatttaa tcaaagctcc catgacaaaa ggcgtaaaac
    31621 aagctgcaaa catcataggg tcgctcttga tttgtctaaa tacctgtatg gtactggata
    31681 atatcctttt catagcttta cacctcctaa acttttccac atatgcttaa ctgtatgtcg
    31741 cgcaactatg tagaggataa tcaacgtagc aattacaagt accatatcaa acgatagtaa
    31801 aacgcttttt ccagttataa gattcatgca agctgtgaat ggaaaaaagc ggaacagata
    31861 tggtaacttc acaaacagac caacaatagg tggtgcaaag caaacaattt ctatcggcat
    31921 aatcacaatg agaaattggt tcaagtttga aatttttgta gctgcaataa ttccaagcat
    31981 agtaaagatt gctgaggtca aagccgtacc aactgtgata ccaagtaaat gattgctgcc
    32041 ggaaactacc cctaatatca atgcgattac cgtagaaata gcaatcaacg caactgtttt
    32101 tgaaagtata tattccgata tttttacagg ggataccacc atagcattta ataccttttg
    32161 acttttttcc aaaagaacaa tcgctcccat gaaaaacaat cccattgctg ccgggtcaga
    32221 gtagatcata atagaagcaa tatccgtttt ccaatgttcc ggcaaagcgg caatcccgca
    32281 cacatataaa actgttaata taaagtagat aaaatagaaa ccatatttcc attggaaatg
    32341 tatatcacca cgaattaaac gtcccaatct cattgaagtg tcctccctgt aatatcaaca
    32401 aaaatatcat ttaatgtagg ctcactacta tgaatagaaa gcaaacggtt ttcggaaatt
    32461 aaacttttta acagcttatc ttctgtggtt ctatcaagca gacattctcc ggttttctcg
    32521 cctttatcat agtaggtata ttgtattttg gcagctcctc ttgacataat caaattatgc
    32581 gggctatcca atgcactgac ttttccggca acgataaagg ctacttggtc acatagctct
    32641 gttgcgtcaa acatattgtg agtagtaatg ataatcgttt ttccgcgttt cttttccgca
    32701 agaataatat ctttcattaa acgactattt gtagggtcaa gaccgcttgt aggttcatca
    32761 aggaataata tatcagggtc atggactaac gctttaatga aattcaagcg agaacgcata
    32821 cctttggaaa agtcagcaac ttttttgtcc ccgtcatttt ccaagcctac catatgcaat
    32881 aattcatcaa tagaccgagg ttgcttttca tatagagaag caaaataggc gagattttgt
    32941 cgtgctgtga atttctcgta gcaggttgaa aattcaaagt ctaccccaat attttcataa
    33001 aactgatttg tatgttccct tatttctgta cctgcaactt ttacgctgcc tttatatctt
    33061 gtattaagcc cagttaaaac tttttgaata gttgattttc cagcaccaga gggtcctaaa
    33121 aaaccaaaaa tttctcctct cccaacatgg aaacttacat tctccacaaa cggtttgtca
    33181 gtatagctaa aatacagttt ttttacatca atcattattc attcctcctt taactttatg
    33241 acgtttatag atatatagtc atataaactg taaaaaattt gtttggctac ctgcctgtca
    33301 aataaccaaa caaaattgtt actctgctac tacttgctga ataataccat tcactaaaag
    33361 attttgtatc atattcagtt cttcgtcctc tttgaattgt tccaaacagg ataaaacaga
    33421 atacagggat tttatgataa ctgtatgcgg aaccaaaaat ataaccccta aatcttccag
    33481 tatctccaat atacgaatat cactgtacat atgattttca aaaatatctg acgatatttt
    33541 tctttgcaga tagtccatta aagataaatc aatctgcgaa agaataggcg aagtaatcac
    33601 ccccgttaaa cttagcataa taacgtcctt tgctaaatct ctatggttct tatacttttt
    33661 ctttttaatg gttgtttcta tacttgtaaa taactctttt tgtaagcgaa aattgatttc
    33721 aacatataga tgttctttac tgggataaaa agcatagaaa gaaccttttg atatgtttac
    33781 agcagctact aaatcatcaa ctgttacttt ttttaagccg tgtaatgcaa acagcttttc
    33841 tccctctatc aaaagtttac tgtttatgat ttctttttct tgttccgtaa atcgtggcaa
    33901 actcataacc tccttatgac taaaatattt atatcgtcat gaaaagtata acatttactg
    33961 attatataat caatatgcta tccaagatat ttatgcaagg tagcttccaa tgcgtttatt
    34021 gtagtgtccg tgtcctcatt aaaaatcatg tgacgattaa gctctaaagc aagtaattcc
    34081 ttttgcggta catgaattaa atcaaatact ttgttggtat aatcaaagct aaaaagcggg
    34141 tcgcctttgg cagtaattaa aataacagga cattgtatag ccccattctc caggcacgat
    34201 aagtcagcat taaataagct ggcgacaaat cttaaaggat aatagggtaa aagcataggg
    34261 tcatttaagc aatcttgttt tgcctgttca ctataaaata ctctgtcata gtctaaataa
    34321 gcgtctcctg gaaccttgta gttaggcagc aattttccac ctacacgaaa cccccactgt
    34381 atcagcccca taaaacggtg cgaccatgcg ggatattttg ttaagctcat agtttctttt
    34441 aatgtagtca gcatgatatt atgtggaaaa acggctttta accttgtgtc cctacctgcc
    34501 gccattgcag ctaaaatccc gccttgactg gaacccatga cagctatgtt ttcaccgaaa
    34561 gtttctattc cgtaagttgt tgcgtcatat acattatgca acatatcttc cattatataa
    34621 ttttttttga ttttagggct ttttccgtgt gaaagatagt gaacacctat tatattaaac
    34681 cctctttcgc tcaatccaca tagaaaattc tcatacatga gagggtgtga catagtacct
    34741 ggcaaaaaaa caatggtagg actacttctt tctgtatacc aaatagaaag tgttatcgga
    34801 acattatttg aagaaatgcc aacttcttta tacatacacc atgcctccta tatgactgat
    34861 ttaattttat agtcatatta tagcttattg caagcagaaa gtcaatagct ataaatatat
    34921 gatttaaggt aaaattgtca agaaagattc cattgcttaa aatacaatga aatagcttgt
    34981 cagtgcagta catttgctac agaaaaggca gactatatcc gaaaatagag cctgccaaca
    35041 aaatgtctta cataataatc ttccagttac tatccttata tagtacaagc tcatactgcg
    35101 aaactaaagt tgcctttgtc tgattatcaa ggaatttcac ggctactttg actttcacat
    35161 tgtccccgtc ctttgtaaat actgggttta ccagttcaga ataaaggtag tccctgccga
    35221 taggttcaag cacatttccc gttacataat aagcaagctc tttttctgtt gctgtcgggt
    35281 acagattaaa gaaagtttcc aaaaatgcgg tagcgtcatt gacggtatct gcgtccacgc
    35341 ttgcgtcggc ttcttgtgcc tttggctcat agtctgattt ttcaatcgct ggtgcaaggg
    35401 tagggttctg aacgataacc atatcgcctc tttatctaca tggattttaa cggtataggt
    35461 tgctttgaca ttcgttgtct gttcgccctc ttttatctgc tgatctactt cgtaggtagt
    35521 aaataaaggc tctcatactt taaatcttga tttctttgtc attgtataaa tatattcctc
    35581 tggcgtgggt cttttgttgc caaaatattt tttgaaattt gcctgtactt ctgggtcggt
    35641 gctattcata gcttcatcaa tggtggcttc tatgttttct tgaatttccg atagtggaac
    35701 accactcaat tttgcccctt gtttcagcat tttttaatat cccgcttttt agtcttatca
    35761 tggtatcact ccaatcctat tttttagcta cggctaaata actgaattta tttatctttt
    35821 gatatggtat acattgaaaa ctgtcatgtt tcaaacaata tcagacaccc taaagggaaa
    35881 tacataaata ataaagataa tctttgccat aaaccctcaa atgctaaaaa cgtattccta
    35941 aaattaggtt tatctcccat aacaaaacat gaaaaacaca atattgctaa tataaaacac
    36001 ccaatgcaga aaaatgaaaa ttttatgtca tttattttat acgcataaat tcctaacaga
    36061 agcggtgcaa atgacaaaca cataaatcca acaacagagc cgtaaccatg tattttttct
    36121 gaaagagtat taagactttt tacttctcca acagagaata agccagaaag aatacagccg
    36181 ccgattgcat aaatcagcaa tactataaaa aggattattg caattagcct tgaccccttt
    36241 gaaacaaccg catatacctt aaaattgatt actgctaaag tcattccaaa gattaccagc
    36301 catgtattat atacattatg caacggtgct ttactatttc caagaacact cataacctgt
    36361 gttaaatgac tatatcctct atatgtggga gctaagataa aaggaataag taaatctaaa
    36421 atgaaagcta tcggaagtat aagcataatg ttatttttca tcatctatac cgttgcttaa
    36481 tttacccagc cctttcaata ttttcacagc taattctttc ataacatctg ctttcacaat
    36541 agctattccc tgttcttcgt cgcccagcac taataatgta tctcctgctt taatatcaaa
    36601 aatttgtcgt gcttcttttg gaataacgat ttgtcctttt tctcctactt taaccgttcc
    36661 aaatatgtgt tgtccctttt tcattggcat aattacacct caattctaac tttttatttt
    36721 tgttatactt ttcatactta caaaacaagt ataacataag gagtgtagtt tgtcaaattt
    36781 aaggaagcgt gtaccccttt gcagatatac acgccttatt gattaaccta tgattttcca
    36841 gttcccgtcc ttatgaagcg tcagctcata ttgtgaaatc tgcgttgcct ttgtctgatt
    36901 gtcgataaac ttcacagcaa cctttacctt aatgttatcc tcgtcagccg taaatacagg
    36961 attgacaagc tcggaataga gatagtcccc gtttatcggt tcaagggcat ttcccaccac
    37021 atagtaggca atctccgttt ccgtagccgt agggtacagc ttgaagaatg tttccaaaaa
    37081 cgccgtagcg tctgccgtgg tatcagcgtc cacgctgttg tccgcttcct gtgcctttgg
    37141 ctcatagtcc gatttttcca tagcgggagc aagggtcggg ttctgtacga ttaccatatc
    37201 gccgtcttta tctacatata ctttgacggt ataggttgct ttgacatttg ttgtctgttc
    37261 tccctctttt atctgctgat ctacttcgta ggtagcgata aaatcgtccg ttcccgactg
    37321 ttctatatcc caaatcaaga catttgtaac aatggagctg gtcggtatat ctgttcttat
    37381 ggtatcaagg ttcaagtcct gcaattcctt tgtcagatag ttactgattg cctgcgccct
    37441 tgcttcgata gcttccttgc tgttgtccca tgtgtaatag gacttacaga agttcttcac
    37501 gaaattttca atcccgttgg tgtcatttaa tctaagctgt atcgtttctg tttcatgtac
    37561 ggtgtgcata tcaatagcgg taaagttctt ataaactcca aagctcacac ttgcgataag
    37621 cactacccat aatgcaatca cagatttctt atgtgtgcct accttgacag tacgcacttt
    37681 ttttctttta ctggtttttc tttcttttta aacatggttt ccaatccttt ctactgtttt
    37741 attcttccag caccaatcaa gtgctgttgc caatacgaat tattaaggtc ggtatatccg
    37801 atagggtcgc ctgcatgata catctgatta tttcctacat agataccgac atgggttaca
    37861 tagcttcctg cattgtaggt actgtggaaa aagaccaaat cgccagcctt tgcctgcgat
    37921 agtgggatat gctgtgttgc gtcatactgc atttgtgcgg ttctcggtaa agagatacca
    37981 gccttgccat aacaccactg ggtcagtccg ctacaatcaa aagaagtgtt cgggttactg
    38041 cccccataca cgtatttcca accttgatac ttcaacgctt cattcattat cttttgtgcc
    38101 agttcgcccg atacttgatt gacgacaagg tactggttca caagctccac atagaacata
    38161 tttccatagc cataacgcca gcccccccgt tttttgccac agcgataggg ttcgtatagg
    38221 ttactttctg accgctcgat ttatcccttg cgaagttttc tgcaaggtta aaagtatgtt
    38281 tctttccttt tcctgccaca tagcccacat aacccccgcc ataattgtaa ttttcatatg
    38341 cgtacatatc agtacctcct tgtataattt ataatcaaag tatacatgaa aattttttgt
    38401 atagcttgtc gcacaatgcc attatcttat aagaaatcaa aagatataaa aaaccaattt
    38461 tgtctatatg gtaactttta ttcctttggt actttttaat gagccatatc taataaattc
    38521 atcacatatc ttttaattct tttcgtatca tagacctttg gaactgtatt gtccattgct
    38581 tcgtcaatcg ttgcttcaat attagccata acatcaacta atgatatagt cagatttcaa
    38641 aatcccatat agataatagt cgcaataggt atttaccatt ttcccctcac gaatatgtcc
    38701 ttccctttga aaacccactt tttctaatgt tttataagaa gcatgattta aaatatgcac
    38761 atctgtccat attttttgta attctgtttt tgaaaaacaa aattcaatta cgcctttcaa
    38821 cacttcactc attagtccat taccttgata ttgcttagaa agtctgaaag ccacttttgc
    38881 cattctatca ttttcaataa ggtatatcca cagttcgcca atcactttgc tatcttgttt
    38941 atggacaatt ccccaatgaa aacttttagt agctttctct ggcttattaa aaagtaattc
    39001 tggatttttt tcgttttttg aaggacgttt cccccaatac tcgtataaag aaatatcact
    39061 aatccattct ttcaaatcat ctatgtcatt tttattcatt gttcttaaaa taagatgttc
    39121 tgtttcaatt actggttttt caaaaatgta atcctgcaaa ctcatggttc atcactcctt
    39181 aaaaattaaa ttcgttttcc catgcaaaag aagcagattc ttctaagctg tgaggccaat
    39241 gactacacca atctggaact tgcggatttt ccactctatc caaactcggc gtgtatggtt
    39301 taatatccaa gataggcgtt ttgtcattag cgtcaatata agcaatttgt atcagcccac
    39361 tcatataatc aatattgata atttctactg ctgataaagc aatcggattt gggcgtactg
    39421 gcgaccttgt tgcaaaaatc cccattttct ctggagcttt tttataaggt tggtctgttt
    39481 ccaatacgct tctaaaatcc gcattgtcaa aatcgctgaa ccaccataaa acattgatat
    39541 gactgaatcc gtctaattcc tttaatgctg gaatatatgg tgcttctagt tgaatgaaag
    39601 cacctttttc attgttgtag atagtaccta ttgaatttac tttataagtt gtcattatat
    39661 tttcctccgt tttcttcttg ataaataaac gataaacctt aacaccatgt taaagtcaag
    39721 gcagattatc attttttaca agagattttc taaaggaatt tggatttctg ttagatagtt
    39781 ctctggattt ttctcgttcc acggtccacg atgaactatt tgacggcttg aacctttcat
    39841 cttatattga ctgttttctt gaagccattt tgcaaatgaa cgataagcat aagaaatatt
    39901 agaaaaatct ccatatacca tagtacaagc catcatagaa actggttcgg taaaacgaaa
    39961 cttaaaatct cctgtagctt ttcctatttt atttacaatg gtacataatt ctacatcaac
    40021 atctttttct ttatattctt cattgtgata tattgagaat gtttcatcag aaatatttat
    40081 tttattttct ttagcaaaag cagagagttc tttccagagt tcgccctcat aataataatt
    40141 tggtatttct tttcttaaag ataaaacgca ataactcggt atctctttta tggttatgtt
    40201 aaaatacaat tcacttttgc catgcaacaa ttctttttta gcaatctcaa ttttctttag
    40261 tttttcttgt tctttttgta tattatccat aatttcaata gactttttat caagttgttc
    40321 tataatggta tcattactgc aagcgagggc taatgcaatt tcagcgacat tgaaaccact
    40381 atcacgtaaa tatataattc tatttaaagt tgagatttgt tctgctgaat acatacgata
    40441 tccagttaat gaatcaattt tagcaggttt taataaacct gtttcatcat aataacgcaa
    40501 catacgagta gaaacttgtg tcaattttga aaactcacca attttgaaca ttttatccct
    40561 ccattcatgt acctaattat aattattgtt aatttatact tcaatataaa aagggcttgc
    40621 ataccataat tatgatacaa gcccatttcc atgttatcct acaattttcc agttgctatc
    40681 cttatgtagc acaagctcat actgcgaaac ctgcgttgcc tttgtctgct tatcaaggaa
    40741 tttcacagcc accttgactt tcacattgtc cccgtcttta gttaagatag ggtttaccaa
    40801 ttcagaataa agatagtccc tgccgatagg ctcaagcaca tttccagcta catagtaagc
    40861 aagttctttt tctgttgctg tcgggtacag cttaaagaat gtttccagaa atgcggttgc
    40921 gtcatttatg gtatcagcgt ccagtttacc gtctgcttct ggtgtctttg gttcatagtc
    40981 tgatttctgg attgctggtg caagggtagg gttctgcacg attaccatat ccccgtcagc
    41041 gtctacatgg acttttacgg tataagttgc cttgactgct gttgtctgtt ctccctcttt
    41101 tatctgctga tctacttcgt aggtagcaga aaaaatgtcc gttccaaact gttcaatatt
    41161 ccacacaatc acatctgtaa cggcagagct ggtcggtata tccgttctga ttgtatcaac
    41221 attcaagtcc tgcaattcct ttgtcagata accgctgatt gcctgcgtcc ttgcgtcaat
    41281 ggcttctttg ctgttattcc atgaataata ggacttcgca aagttcttca cgaaattttc
    41341 aatcccgttg gtatcagtaa gtcttaattg tatggtttct gtttcatgta tggtgtgcat
    41401 atcaatagcg gtaaaatttt tatatacgcc aaagctcacg cttgcgataa gcaccaccca
    41461 caacgcaatc acggttttct tatgtgtgcc taccttgaca gtacgcacct ttttttcttt
    41521 gatattttct gtctgtttct tattcttctt aaacatattt tctaagtcct ttcttttatt
    41581 ttactcgtcc tgcaccaatt aagtgttgtt gccagtagct actacttagg tctgcatatc
    41641 ctatcgggtc gcctgcatga tacatctgat tatttcccac atatattccc acatgggtaa
    41701 catacgaacc agcgttatag gtcgaatgga aaaataccaa gtccccagcc tttgcctgcg
    41761 ataaggggat atgctgggta gcgtcatact gcgtttgagc cgttctcggt aaggaaatac
    41821 cggcttttcc atagcaccat tgcacaagtc ctgaacagtc aaaggaagtg ttgggattgc
    41881 taccgccata cacatatttc cagccttgat atttcaacgc ttcattcatt accttttgtg
    41941 ccagttctcc cgatatctgc ggtacagtta aatactgatt gactaattcc acataaaaca
    42001 tatttccata gccataccgc cagccaccat ttttcgcaac ggctatcggg ttggtgtagg
    42061 ttactttctt accgcccgat ttttcacggg cgaagttctc tgcaaggtta aaagtatgtt
    42121 tctttccttt tcctgctaca tatcccacat agccacctcc atagttatag gactgtatcg
    42181 ctacattcaa gtcgtcgata ccttgatttt tgcaggaaga aagcagggac gcaaaatatt
    42241 tacacccctg cttgattgag ctttccgtat ctaaggaatt aggcggtaaa ccaagacttt
    42301 ctgaactctg cataacatct tctgccgtac cgccactttc tacttgaatg atagccagta
    42361 acacattgac atactcggag ataccgtttt ctctggcgta cttttctacc ataggctgat
    42421 gtttcaaaac ttctgcggat aagttcatac ccgtaatgcc cgaagaaaag ttgctgttat
    42481 cgtcatcggt gtcagcacta atcagcaccc caaagaaaag gacaatggaa aagagaatag
    42541 gaaacagact gccaataata gcgatatgtt tcaatttcat tttttcttct gtcctttctt
    42601 cgttacaagg ttacggttct tttcagtatg ttctgttgta tgttttgatt gttcaaaggt
    42661 ctgtgtcttt gtggtacgct ctgttttgta attctggcgt gtttcctgcg ataccacttt
    42721 ttctatattc tgcctatata ctggctgtgc cgactgggtc gtttttctat cagaagcacc
    42781 agaagataga ggacgttctt tcacaccatt ggttttgact ggctcactta ctttggaagt
    42841 tgaagttgta gcaggtcgtt tgacttcctg tgttttctca acactcggct ttaaagctgt
    42901 agctggtcgc tcatgggaac gggtagctcc cgttgtcgct gatccgtcca tctttcgtgc
    42961 ctgccttgct tcttgtgctt tttgaagttc catacgcttg tcagcgatat tcttcttatg
    43021 ctgttccagt ttttcagagc gtcccgtctg tctggactgt tgttcctgca ccacgccacg
    43081 cttgaagtcc gacacgcttg tttttgcttt ttcctttgca gaatggacgg cataagcggt
    43141 ctgtgtcggc atatccttaa tattttcttt gacagcgttt gccttgtcct tgactttatt
    43201 tttcgtatct aagacagcac ccacctttga acctgcacgc tgtcccatac tggaagaaga
    43261 tgtatttccc cgactgtctt ttgtggaagt tttccttgtc ggctttccat ttgagattgt
    43321 actacctgcc accgcaccag ccacaccgcc agcggtaaca gcaccagcaa gccgtctttc
    43381 catacgccta gccctatgtg ccatatataa atatggtctg cggaatattc ttcgtcccat
    43441 gctttgactg tcgttagcgt tcaaggagaa catactcatt aaatcgccca gtttcatgta
    43501 gataccagcg aaacacacta tctgtaagaa cgccaccata aagaacggat agtccgtgga
    43561 tatgttataa aacatactgg aaatactgaa cgccaccgtt acaataagcg taattccagc
    43621 ccttgtcatt atggtattaa atactctcac gattgcctgc tttgccatgc tttcatagct
    43681 cggtatcata gagagcagga aactaatagg caggaacata gcgaaaataa taaaaagtat
    43741 ctggctaaat aacatcatgc ccgtaagcaa gaatacaaat atcgtgatac ccaagttgaa
    43801 aaacaagagg aagaacacca tacctaaacg atttaccacc tgcggtatgg tcagattgtt
    43861 gttctcgtta tcctcgattt ctgtttttac gacttcctct ctggttttcc cgtcctcgtc
    43921 ctctgggctt gccgatacaa gagcttctac acggtctgtc ccgatttctt ctgcattgct
    43981 gtttccaaac tgtaagagta gccacggctg ttccacttga atagagaata ggctgtccct
    44041 tatcaagtcc acgctgtcct tgccctcgct gtcggaattg gggagcatga tttttgttcc
    44101 caagtccaaa gaagcggtgc tgatgtctga tgaaaattca tttatctttt tgatgtaatc
    44161 gggagcatag gcaataaatg aagccgacaa tacaaacacc acaacaaagt tgataacagc
    44221 gtgaagtgct ttgctggttt ctctttttat cagccccgtg taagcaacat atagtcccac
    44281 cactaagata atgagtagca ggaagcccac atagaagccc gtagaagaaa aaccgttctg
    44341 tgttacgcct gcaagtgtct gtatgctttt tccgatactg tctgccatat cgttaataaa
    44401 atcaagttta taggcttcct gcaccacata gcccgtggca ttagataagt aaaggcttat
    44461 cgtccagaca aaattagtga tacagtaaag cccgtactgt accgattttc cgataccgtc
    44521 cagccagttc cacggcaacc acgaccagct attatccaca taaaaatcaa gctggtagtt
    44581 ggaaagagga tattttgagt aaagattttc cgcatttatg gtatcgtcca caagtcccgt
    44641 tgcatgagcc atcgtcccaa gaagtgaaag caggataagg gaaagtgcca cgacaaatag
    44701 agccattttg agaaagtgga aaatcttctt ttttgtgaac gcactctgta tcctttcttt
    44761 catcactcca cctcatttct ctgtatgggc ggtctggtat caaaggcatg taagagttct
    44821 tcaaagactg ggtgtatctg caccacaccg acacgcccgt ataagtcctg caataaacat
    44881 tgtccgttct ccaaatctcg taagcgtttc tggttgtttt cgtcgtcctt gtcgataccg
    44941 aaaaattcta aggtctgctt tatctcgttg atgtcagtag aacggaaagc gaattttaag
    45001 ccgatattat ttttcaaact ttcctttgaa acatcatagg cagactgggt aacaaaataa
    45061 acgcctgcct gcatagctcg cccagcacgt accagcttgt tagataaggt ttcgccttgt
    45121 gccacgttca agaacgccca cgcttcgtcc aaatcaacaa tcttaaaaat gcttctgtcc
    45181 gaatggataa aatcaagggc aaaggtacta ataacaatca acattgccac cgataataat
    45241 tcaatggtcg tgtattcgtt aaaagtagta tctttatctg gcaataccaa atctgctact
    45301 tgaatgatat tgagctggtt gtcaaggctg atagcatttt ccaccgttcc gtccgaaaat
    45361 aataaatgtg caaagtcata gtctgtaaaa ctgtcgatat ggtctgcgat atttcgtgag
    45421 attgcggtat cttcctttct aagttcgtct atcacatgaa gcaagcccct tgtttcactc
    45481 tgggtaacag aacgcaccac cttacgaagt acggggaatt tttcgccgtc cctagaagaa
    45541 atacccgtaa ggaatgtcag tatatcaatc gcaagacttt cagcgtcttt tacattcttc
    45601 ataatcacga acgggtcaag aagtcctgcg ttgtccttat cgctggtaag gtttacgata
    45661 ttgatttcat gtgcaatctc tggaagtgtt tctttccagt tgccacgctc tgatttcggg
    45721 tctaggataa ccgcttgtcc cccaaagaga acagagtaat acacaagcag gttgttacag
    45781 aacgactttc caccgccaag cgaacccaca aaggcagaag caagggcatt tgtaaccgtt
    45841 ccttttacgc cttgactggc aagggacggt tgcaggtaca catttcttcc cgtatcaaca
    45901 gaatagccca tatagatacc cgtagtttcc cctaactgct gtgtcgcacc aaaaccaagc
    45961 ccagctagaa aatctgattt tacatactgc acatagtcat tgatatatcg cttgctggca
    46021 ggtaaaaatt cagaatgaag ccccagcata tccccagcag gacgcaccag ctttacattg
    46081 aggtcgtcgt aaaagtcctt gacttcatca caacggcgtt tcagctcgtc aagatcgggt
    46141 gcagacaccc gtataacata gcttagctta tacatacttt ctttggtctg gtctaaatca
    46201 gtttccagct cgtccacgct gtctaatgcg tccactacat ttgagcttgt ttcacttcct
    46261 gcttgatagg cgtgattgtc aaggtctttc aattcctttt tcttgttgcg tactgtggta
    46321 agagcttttc tattttccac gatttctaca ttcatgctcg tatcaacggg gaatgtgaat
    46381 tgctgttgct ggaaataaaa gatttcagag gacggaaaat caagttcccc cacaatcgca
    46441 ttgacggtaa agtaggacac atagctttcc ttgtcctcat gttccagtcg taagtatcgc
    46501 tggctttcct caatcacaca tctggtcgga cggataaggt catagtattt tatcagcgtt
    46561 tcttttttgt atttcttctt tggtagctga tactcataat cttcataggc aataccgtcc
    46621 ctgccgtaga gatgttccat gagatagcca aaatcatcga tttctaatcg gcgtactttg
    46681 aaacgccttg agattttatt ttccaacagc ttctccattt tcatgtagcg gttgatttca
    46741 tcattcggca tggaaacaaa gtcattcatc agcgtgtggt tcacttcatg gagaaactct
    46801 ttgaatgtca gccaagccga ttttttgatg ttcttcagat tgagctgttc ttccgttacc
    46861 atgagcttga agccaagaaa aaagcggtag tccacttgat tgtccccaat catagatact
    46921 aacgcttcgg tctgttcatc aatttttctt accgccacct cacgaagttt ccccgtaacc
    46981 aatcgtttgg actgttcctg catacttcta attgagcttt ctgtagcaat ctgcaaggca
    47041 tggattttac cctcacggga ctgtgcaatt agctggcgaa agctgtcatg cacaataaat
    47101 ttctgttccg cagatagaaa gctgtaattg taaggtatca gctcatagta ggcgtacact
    47161 tcattgtcct tgttccagac aagattgttg tcgatatatt taatcgggta catagttcac
    47221 actccttact gccgtaatcg tttcgtttaa tatctgctta tccagtttta cggcttttcc
    47281 tgcataagtg attttgggtc gcagggcata ggttatctgt gatttcaaaa aactgtacgg
    47341 cttctttccg tcaaacgttt tctgggacat aaaccaagta agagcaacgg gaataccgaa
    47401 gtattttaaa aatgctccct caaccatgga aagtggtggc atatccccaa acagaatgat
    47461 gaaaaactct gtaatcacaa accatgtaat ctgtgtaaag gtaactggaa aaggtaggtt
    47521 aaagtcattg attgcatata agactttttc tacattccag atacccgtgt agcttttaat
    47581 ctttttcaag ttctttcaac tcctttcaaa atggaaaagg acagccatgt ttcagactgt
    47641 cccttaaaag agaaatacgc tgtcagtagc aacaatactt gtcgctgatc ttccagcgtt
    47701 aatatggtat ctcgcacata cctcggcttg ttgcaatgaa gcaaccgccc atatctaaat
    47761 ctcgcccata ggcttcatag tcgatatagt tctgcaagct gggtggaatg tctccaagtg
    47821 cctgcaattc gtctatgtag tagtaggcaa cgtctgtcat ggtttcacag tcgggataat
    47881 aataaatatt gtccttgtgt tccatgactt cttccagcgt cccgtaatga ccgataaatt
    47941 cgtccaaaca ctctacgatg tagtcgggaa gttcctctat catttcatac atttcattga
    48001 gttcttcaat ggaaacgtac tccccaatct caatagggaa gttgtcggta tcatggattg
    48061 cgtattcttc gcattgttca ttcaaaccaa ttttttcttt cacatcttcc tcgtcaatag
    48121 ggaaagtgaa ccaagcaccg actaaatagc cctcattgta cttgccaaga ttagcgatat
    48181 aaactgccat atcatcaatc ataagtacca cctcatttcc tactctggca gttcaaagac
    48241 agcgtgttcc gttacaagat agcgtctgtt ttccagtaag tgagaagcat aggcttcaaa
    48301 gtcaaagtag tattcttgac agtcctcgga taaatgtttg aacgtcgggt cgttcatcag
    48361 cttttgtctt gcaacatcta tcatgctttt gcagtcggga taaaccgcaa tcacgttcct
    48421 gcaaatataa agtgcttcta agttctcata tacggtaaga agtgctgtat attcttcctg
    48481 catatcgctt gaaagctggc ggtacataaa atctaattcg ttgagctggt acacgcttgt
    48541 atgttcgtgg acttcatcag caaagggcag taccttttca ataatgcggt aatccccact
    48601 ttctgcaccg acacctaact tttcttcaaa ctcggcaaca tctatcggca ggtcgaacca
    48661 gtatgatgtt gtttcttcgc cagttgttcc gctcgtttcc accagcaccc ttgtttcctg
    48721 cattgttcct cacgtccttt ctgttgtttc atcacatctt ttaaggtctg gcatgacata
    48781 ccaaacacat agttagaaaa atcttctaac tgtttttctt catagtcagc aaagttcagt
    48841 tgcttcattt catgccacac tttacgtttg taagagggca ggtctgaaat gttttcacag
    48901 ccaacctttg cctgcatatc cttaaaaata tcgttcattt catcactcca atctgaaatt
    48961 aaagtaagaa aaaagcagtc gatcttaaga ctaactgctt tgtgtgtatg gatatgaagt
    49021 tgtcaaactg aaatttaaaa atcacttttt cttgataagt ttcagtttta tatttcttct
    49081 gtcggcaatc ttctgaaaca tctctaccac atacaagcat tgtgccttaa atggtagtaa
    49141 ggtcaattcg tcatttgatt gtatttgttt cattgcttct tgcaattctg aaagttctaa
    49201 taaattgact gctacacaaa agaaagtctt tctacgcccg tcattatagt tagaaagtaa
    49261 gtaagaaaga atttgtgttt tttcctgctg ttcaagatta tattgctcaa tgcctatgat
    49321 tttcgctctt tctaaatctg ctttttgtcg tctgctcgta ataaaaatat cataatcatc
    49381 aatgtgttga tatttttcac acggatattg tttgcactca tagcaatatt caatcattcc
    49441 atgttcaaga ctacatcttg caattttaca tgattgatta ccattgccac aaccgccaca
    49501 atgatttcct aaaagcatag gacacaaccc acaattcaat ccacaaagag agaataattg
    49561 gtttttgcgt tcaaatcctt tcatagtatt agtctcctaa cttcaaattt atctttgtat
    49621 ttattgactt ttacaaactg gatttgatga aagagagtct atattgaaat ccagtatcgt
    49681 tggataattc cactttcact aagtcctact gtatcaggga tttctttttc caacacgcca
    49741 ccatttctta caatcgtttt ttgtgatgct tcattctttt tatcacatgt tagtaaaact
    49801 ttgctttccc caaactcatg acagataggt aaaatcaatc ttaacatttc ggacgcataa
    49861 cctttctgcc gttccgatgg acgaacacta tatccgatat taccaccaaa attaaagagg
    49921 aagtcggata atgggtgtcg aaaatcaatc atgccaataa catagttatc tttttttcgt
    49981 atagccaaaa acacttctga tggaacatat ccgtccttat attttgcttt taatcttgct
    50041 tcaaaatcaa tccattcctc aaatgattgt acattttcaa gaccagcaca accgtcaaaa
    50101 ctatctccat tttgtgacat ttcttcttta tatgacataa cttggtctgc atattgttta
    50161 gacggtctga ctaacaatat tttacacatc ttacttctcc cccaaaattc ccatttgtcg
    50221 ctttcttcat atccacatta tagcattctc atatcagcac cacaataaaa ttttacgcac
    50281 cgataatctt attgaacagc tctaacaata cgtctttcac accgccagca ttgaatacca
    50341 aaccgacagc aatcaaggca actaccaaga agccgataag ttttgaaaac tcacgtttga
    50401 accctaagta gataccaata accacaatcg ccataagcac taagctctgg gcgttactta
    50461 aaaaccatgt gtataaattt tgtccgaaat tcatttaatt gtcctctctt tctttgattt
    50521 cttccataca tctgtcagct tctttgccga cagttaaaat gcacattaag actacgccga
    50581 tacctattcc aaatgatacc agcaataagt ctttcaataa ttcccacatt ttttcatcac
    50641 tcctttacgt tggcaaccat ttcttcaacc gtggctgtct gttgcttgat tatctggtag
    50701 tgtcgttcgg tcagttttgc ctgcttttca atcgtttcca gatactccgt ctgattgtcc
    50761 ttgtctatct gtttcaacat cttcaaggtt ggtgcaacct gcctttgtag ccagttgagt
    50821 gtacgctcca atgtgtaaga ttctggtttt gtcgtcagct ttacgggcgg tcgcccctcg
    50881 ccaatgaacc aagcccagcg tacattggtt ttccagtcgc tttttcgctt gtctggttct
    50941 ttatcagcga agcggatata ttgattgata atagaaaatg ctgttagttc tgcgtcataa
    51001 tgtgtcagca gttccacaac agcataatag gcacgttcat ttttcaaacg tatctcaaat
    51061 ctatttttta tggggacttc ctcaatcggt ataccaagtt tggcgtactg ttcgtaattc
    51121 ttttcataac aacagaagta cacttcactt gaaaatgaac cgatatacag cgtgtgtccc
    51181 atatcgtatt tatccgtttc attatgcttt accagttccc cagaaccata gcttttgaaa
    51241 gaacggaatt tagaaataca ttcctcatgt tcacactttt gtatcagctc tggtatatcc
    51301 agcaatcccg ttctgtcatt gattgccaaa tcaagacgct tcatcacacc gccctcaacc
    51361 aatgcgtccg tgaagaagtc ataccagctt cgctcttgtg ccacaaggta gctttcaaac
    51421 tggcgacagc ctttgccttt cagttccagc aggacacctt tttcctcgtc ctgcgataca
    51481 tacacaaaca catctccgat atggtaatgc tcggtgtatt tgtaatgtcc gtaatcttcg
    51541 tgaagcatat agtcgatatt gagctttaat atatccttga tgatatgttt tatatccatt
    51601 gtgggaaaac ggatacgcac ataatcaaac agcaggaaca tgggattgtc gggattgaat
    51661 ttttccaccg cttctaaaag cgtatgtttc atgtcctcgg tcagcgttac ctttcctgct
    51721 tcgatacggc tcaaatgttc acggctgata tttgccatga ttgcaagcct tgtctgcgat
    51781 acgccgtatg cacagcgttt ttctttcaag gcggttaaaa aatcagtttc attcagttgt
    51841 cataccccca atcgtgataa ttttgtgata ttgtgataat ttcaaggctt tatcacaaaa
    51901 ggcgaaatgt acgaaaaacc cttattttat gcgggcttgc aaaggttttt gtggaagttt
    51961 ccgccgtaat tgtgcccctc tgttagataa tgaggggctt gtatctgctg gcgtggctaa
    52021 cgccacacca gcaacggcta gtccgtgccg tcgcctttcg cttcgcacgt cgccgtcccg
    52081 tcctgccttg cgtatgcaag agagccgata gtctgtaaaa aatcatgtcc tttcgggact
    52141 aaaggcgtgt aaaattcact tatgacactt gttcccacat cacaatagcc acgtcccttg
    52201 atacgcttct ggaaaaactg ctttttcaca tcagagccga acagcatacc gtaacccaat
    52261 tcgctgatac gccccaagcc aacacggaag ttaaaattat ctctgatacc gtccgagaaa
    52321 tattttgcgt ctggacgctg gcaggcaaca ataaggaaat atcctgcttg tcgtcctaac
    52381 atgacgattt tctttagctg gctaagtagg ctcacacttt cttttgtccc cagcatttca
    52441 aaaaacgcca catattcatc aaagataaga aagcagggcg gtaaaccaag ataggcatag
    52501 ttttcgcccg ttttatagtc tgggtgtcgc ttcatttctt tgcttctttg taccatgccc
    52561 tcataaaagg cattgacaca atcaatcatt tcttctttgg tgtgatacac atttcccatg
    52621 accgtaccta aatctgcaag gtcagcgttc ttcgggtcta atatgtaaag aacagcgttc
    52681 gtatgtaaca gagcttcaat aagcgtcagc agaaaatacg ttttaccgcc accagtccca
    52741 ccagcaatca gagcgtgagg gagtgcgtca tattcccata caagattttt catcagccta
    52801 agacagccgt tttctgcttt cacttcatca atggtaatgc ggttcgctat catgtcataa
    52861 agcatggtgt attcgatata accgtcatgt aacgtcttgt cggtcagctc acaatacaag
    52921 ccactttcca atttatcctc taaccgtaag agctggtctt gatattttcc taatgtaatt
    52981 tcacagcgga tatgaagcag tcctttttcc atttgataat aaatctttgg aaaccagatg
    53041 attttttccc ttgatctgct ttgcaggtca gtaaaaaaac cgctgtcttg tacggtatca
    53101 gcttcatacc acttgttttc cagtatcatt cgtgccagct tttgacggtg caggagcttc
    53161 ttgaaactgt cataacagaa gcggtaatac agaaaagcga ccaatgcaca aacaccagtc
    53221 gctatcagta gggttatgaa gttgtaagtg gaaagcgtca aaccgttgtc taataagctg
    53281 aaatgctccc aatcggtacg catgagctgt ttcatattca gtagcaggat aaccgcaatg
    53341 aatacaaaca gaagcgtccc tatggaaaag tggtagacaa ggttcttgtc gctggctctg
    53401 atacggtgtc ctttgtttaa aatcattttc ataaatcaat cactcctttc tgggtacgaa
    53461 aaaagcagtc aatcctaaga ctaactgctt tgtgtgtatg gatatgaagt tgtcaaactg
    53521 ggatttattg ttcttaactt ttctgtcgtt ataaagctga cttacgcaca ttaacctctg
    53581 cacagaccag cccagctata tgctccgctg tttcatttgt cagtacaaag agcattccgt
    53641 cattacggca ttttttatgt gtgtcgccat tcaaggcata aacaaaacct ttcacacagg
    53701 tatcactaca ggcattaggg tcaatcagtc tcttgcagtc acgagaagta gtcattttct
    53761 tttgcatatc agcaggaagc gaagcgatta tatcctcata ctttccagcg ttgtctccgt
    53821 aaatccgtgc aaaaacacct gtcttgcgaa acacccaatt cattactgtt ttcttctcta
    53881 gttggtatga agcggtgtaa ccgctttttg cttcctttat tacaaggtca cagccctgct
    53941 caataagttt ggtattaagt ttctcgacaa acacttggtg ttctggcgcg attgccgata
    54001 gaaattcttt gaatgaacct ttttcttttg ccattatctt tttcctccac aaatttcaat
    54061 ttatccgttt cttcatacac atattataac agttgcttat atccgctaca atgaaaatct
    54121 agtttgccag cttatttctt tggttgcccc tgcggtgcat ggttctgtgg attgcctact
    54181 tgaccgcctt tattcttcaa gataatatcg tcagccttga tataccagtc cacattgaca
    54241 cctcgatagc tggcgtttgc taccgtatcg gctacgggat tgacaagctc tacctctgca
    54301 ttatacggat agtccttgac gggaatatca gcaggaattg atacttgtat catacgttcc
    54361 tgcacactac atttcaaatc ataggttctt cgtgtgattt cttcacttgt tgaaccgtcg
    54421 tcattttctg ctcgcacctc atgtctgagg gcagagaact tcaatacacc gaaagttttt
    54481 tccttgtcta atacaatacc gtttgctaat ctcataatca tttccccccc ttacttgctt
    54541 tcctcggctg gcaacatatc atcagccgtt aagatatagt ttgtgaaccc tcgtcctttg
    54601 accgctttac cgtctgcggt aatctttgga ttgataagtt ttactcgctg ttctggtttg
    54661 aaatgctttt cgcctgcttt cgctggaatg gttacgataa tatcatcagc cctttgtacg
    54721 tcagagtaca agttgtagct tcttgatacg ggcgtaaagc gtccgttaat gcgttgctgt
    54781 tcaatcttgt tttcgccagc aaattccaag tttccaaaag tctgcgccat gttcggcact
    54841 acatatttta attccatagt gttttacctc gtttctttct tcttttttaa gtttgttgat
    54901 tgtgctttct tattctggca attagggaaa taccagcaat cccccagata gtaaagggta
    54961 aatcaatgct tcgcaccctt gactatccgt gtgcttgcag gttgttggca gataagccag
    55021 aataagtaaa cgtctgccgt ttgacagctt cggcggagag cgtgattttt cttttctcat
    55081 accgttaccg ccttatgatt tcttcatttt acggcgtttg taaagatatg ttccagccag
    55141 tccaccagca gatgtaagca acattaccag aaatgccatg acatttgtgt tgtcgcccgt
    55201 tttaggactg tctgttggtc tgctcggttt ttctggtgtt gttggcgttt cgggtttctc
    55261 tgggacttct ggtttttcct taaaagtaat cgtctgtccc tcgtcctcaa tgtccttatg
    55321 ctcagtaact tttttcggct cgtttggatt gcttaaatca tacaattctt caaaagttac
    55381 aagctgtttg tcgtcaagag aagtagcgtc aaaggtaaag gtaacttcca ctttcatagt
    55441 ttcgctgtca gcgataaacg tataatcact ttccacacgt tttccattga taagaagctc
    55501 tgcattttct tctttcaaca tctgccagcc cacaagttta tactttgtac caacttccaa
    55561 gccctctaag gttacagtat caatgattgt tacgtctttt cctgcttcaa gctctttgtt
    55621 accgtcctta tcggtagcag ttgtatgtat tttgatgata cgttctgtaa taagtaccgt
    55681 ctgcccgtcg tcctcaatat ctttatgctc cgcaactttc acgggttcgt ctggattgct
    55741 taagtcgtat aattcctcga atgttaccag atttttacca ccaagctcgg acgcattgaa
    55801 tgtataagaa atttccactt tcatttcttc atcatcagca acaaaggtat aatcgctttc
    55861 tacacgtttc ccgtcaatga taatctcggc gttttcttcc tttaacatct gccagccttt
    55921 taactgatac ttagtgcctt ttgtaagtcc gtctaatttg acagtatcaa cgattgttac
    55981 ctcttttcct gcaaggattg tcttttcgcc gtctttgctg gttgctgtgg tatggatagt
    56041 gatttctttt tcgtattcat cagtcaaagt tcctaaatca atcacaagat tatttcttga
    56101 taccacgatt tcaaatggtg gaataagctc aaaacctttg ttgctgtcag agcgtaattc
    56161 ttcaataata taagtatcgt aaggcaatgc acctttgctg tcgtctggtt cagaagttcc
    56221 aaaccacaca ccgtcctcgc tggtctttcc tgcgttggta ttgtgcttat gtgaagccca
    56281 gtcagaagaa gtggagaatt gcccgttatc atcagttacc acaacatgac tttcgcccgt
    56341 tgtcttgctt gtgatcctaa agggaacatc agcaagacgc ttgtgtgtgc ctgcaccgat
    56401 tttcacgccc tcaatatctc cacgtttaat ctgattgtag acagagtggg cttcgtctgt
    56461 taagtccacg atttttccgt tttctgtgat tgcaaaatca atcggcttcg caccgtctgt
    56521 caagtaaccg tttggagctt gtttctcaac gatacggaaa tttccgtaag gcaaaaggtc
    56581 agcggaagta gaagcgatac cctcaatgtc cgtatggatt gtttttacga cttcattttt
    56641 cttgtatagc ttgccctcaa ccaaaacaga attatcattt aaggaaatga tgtcaaaggc
    56701 agtatctttc aaggttgcac ttccttgtgg tttggtatcg cccgtttcta aatctcgttt
    56761 ctgaattttg acaccgccac gaatgacttt gtctgataca gaaaactggt tacttccagt
    56821 taatacggca aggtcgccgt cctcagtaat ctgtgtaagg tataagcctt ttatctgttc
    56881 ttcactaccg tttgcctgca tatatgcacc gtctaataag tagcccgttg gtgattttgt
    56941 ttcttcgcaa gttagtgtac caagaggtaa aaccgctttt ccgtcctgca tatagaaact
    57001 gtcgccagat actttgtatg cgtctgctaa ttttgtgatg tagtgggttg tcccgtcgct
    57061 gtctgtttca gcgattgtct ttgtaaccca tgtacgagta gcttcggcag gaagattgtc
    57121 tttagtgtag aagcctgcat aatatttcca tgtaaactct gcaccctcta aagaagcgtt
    57181 accttgtggg ttggctttct gtgtttccat gtcaatcttg aaaagctcaa tcaaagtatc
    57241 ggtaactttt ggcgtatcag atactttcaa agtcgctgtc tttccagctt caacttttaa
    57301 ggaatatatg gttttatcca ctttaaatcc cattggtgca gtcaattctt tgatatatac
    57361 ggtagtagct ttgacttctg caacctctgt atttccgctg ttgtcggtcg taagggtagc
    57421 aagctgtttt gtgcagtcct tatcagaaaa gacaccgtaa gtcgcaccag cgatagagta
    57481 attcccgtta ccgtctgtaa tgctggtatt attggaagtt ttttgaagtt tggcatttcc
    57541 aacatttaac ttcgcccaga attgccctaa ttcctgcccc tcgccagaat agatataacc
    57601 gccacattta tagcgtcctt tgttttcttt gacaaaggct tttgcaccag caaacacttc
    57661 atcttgcgta gcctttggaa tttcatcata agaagctcgc acgttatcac attgccagcc
    57721 taagtgaaca ctcaatcttt gccagacaac aagctgtctt aaaaggtagg catgattgtt
    57781 gcttatctca ttgtggctgg cggtgtattg cttcacatat tcgatagata aagcaacatc
    57841 acttatctgg tcggcactca tacgggagct tgcgtcagct ctggtcttat agccgttctt
    57901 aaaatctgtg ttaatgtcaa tacagtaagc gtcctcgcct tcaacggtca aatgcccctc
    57961 gttaaaggta gaaccaatcg aaccgtcatt cattaccttt tcaacgatac caacacgttc
    58021 ttcactttcc gtccagtatt gcttactttc tgcatgaaca agtgtagtcg gtaaagcagt
    58081 aacgacagtc gcaaaggcaa gtacgcccgt acacaatcgt ttcaatatct ttttcataat
    58141 tctaaatgct cctttcattt tgggtaataa aatagccgtt caataagaac ggctggaaat
    58201 agaaaaggaa cgtcatttta ggcgttccat agtctatgaa atattcaatt tttttcttgc
    58261 ttcaatactg atgtgccgta ccatatcttt gcatatctag gaaaactagc gtatcaaggc
    58321 tcattcattg gtagtaaatt agtagtaaat tgatgtgttt gttttcttga aaatgctgat
    58381 agatactgtg ttttagcgga taatcaactt tttagtatgt tttttttgag gaaaaaaaca
    58441 aggttttcat ttgtgatttt cttaaattcc aaaattacgc cctccttaaa tcatttaatg
    58501 ctatgaatat agcataaaat aaattatgaa aatgtttttt tgaaattata cacacaattt
    58561 cttcttaaaa aataaagatt tgtgtattta tacacaaatc tctatcttaa ccaatattgc
    58621 tctattattg ctctcataat catcataatt ggaaccttat ctgatatatt gtacccattt
    58681 acttgtttaa ttgttgaata gcaatataaa cttatatctg caatttgttg caattgattt
    58741 tcacatatat gtgttaaagc tactacctta caacccttat taactgctgt ctttgctgct
    58801 tctataactt ctttcgtttc tccacttaaa ctaagtgcaa aaaacatatc ttgttcacct
    58861 aattttccta tatcatgaag tacttcatgt ctttgactat agtaagttat attatcatgc
    58921 ccacctctaa tttcttttac taatatctca cataaaggaa tgttttgacc tattccataa
    58981 actataatac tcctagattt ttgaattata tctgctactt tatttatttt ttcataatct
    59041 attagctcta atgtcttttt tatatctgat tcaaaaccat tttttggctc tttatcctca
    59101 ttttttaact cttcttttaa attatttttt agttgagaga aaccatcata ctccattttt
    59161 ttacatagcc taactatggt atttgggaca gtaaaaaatt tatctgctaa agattgtatt
    59221 gataatttta ctatttcttc tttgtttttt ctcatatatt caattatcat gtcttctgta
    59281 tctgtaaatt tatactcata tttttttgct ctttcataaa attccatagt ttctcctcca
    59341 tctattttat tattcataag taaaattcaa aaatattagc taaaattttc gttaatataa
    59401 ttataattta atatttttaa tttattaatg catactactc tttttattat acataaaaaa
    59461 tgtgtacttc ctatattgat agagatgatt caagttttta ttttgataca tctctatcaa
    59521 tattttttta gaaaatacac atttttttca aatacttatt tttaattaaa actttaggta
    59581 atctattctt tatatataat tattagactt gaaaatatct ttaaaatcaa ccaattcaat
    59641 attatttctt tttaaccaca ttttaacttc cttatcacat agcattgcat gttcatatac
    59701 tctctcttga ataagagaag atgattttat aagactataa tctataaatc ctggatgagt
    59761 aaccatcaat gttatatctt tatctaaatt tatatatttt tcttcaaata atttaacaaa
    59821 agatttattt aacttgtaaa aatcattctg aggatattta cttggcatgc taatacatga
    59881 atccaaataa tagggacaac ttggtatacc gtattctttt gcagcatcac acattgctga
    59941 tattgataca tcttcttcta taccatgtat atcaatatac tcaggcaatt tactcatata
    60001 caacttgtaa gtttcaattt gagcacaaac ttcttcatat acttcttcat aattaaaaac
    60061 ctctttacct gtcagtagtt gcgttcttct tattttgcta gaaacaaatt ttccaccttc
    60121 atctaacaaa ttctttatct taatatgttc tttgcaacaa ggttctccta ttactaaatt
    60181 aacatgtaaa ccaaaacata catcatcatt ttttaccatt tctatagctt gtcttgaaaa
    60241 aggcatattc accatgattc cagcacttgt aacaataccg ttcttataag aagatattat
    60301 ccctagtgta acaccttctg aatatcctat atcatctgcc ctaactacta atttcttcat
    60361 tttatgcatc tgcctgcttt ctcaactcat caagttgcat ttccatctca tcctttactt
    60421 taacaacatc cgttcctatg atgatttgaa ctgatgtatt actcaattta attacccctt
    60481 tggctcctgt ttttttaata ttatcatcat tcaaaatagt ggcatccttc acttctaaac
    60541 gtaatctcgt catacaatta ttaagagtca ctatattttc ataaccacca caatttttaa
    60601 ttatctgttt agccatatat gaaaaattac catgagaaag ttttaaattc ttttcttttt
    60661 cactaacttt atcacctatc tctatctcaa cttcacgtcc tggtgtttgc acattatctt
    60721 ttattattat atatttaaat gttacataat ataatacaaa gaatacaact cctacaggta
    60781 taactaacca cgctttatct ccataactaa aacttaagac atagtcaatt atacttgctc
    60841 cattaaatga acccaagcga gcacccaata aatatagtac aactccacct agaccagaat
    60901 aaaaagaatg aattatatat aactttggag atgcaaataa aaacgaaaat tctattggtt
    60961 cagttactgt tgccataaaa gaagtcaatg ctccagcggt cattgcacct tttacttctt
    61021 gcttcttttt atcttgtgct gttttatata tagcataaga aatagcaggt attgaaaaca
    61081 tcataattat gtaaaatcct gtccagaaga atcctgcatt tgggtctcct gccataaatc
    61141 taggaatttc tcctcttata acttctcctg ttgatgttgt atattcaccc atttcaaata
    61201 atatgtaagc atttaaaact tgatgtaacc cagttggtat tagtgcacgg ttacagaata
    61261 aaaacaagaa tattcctatt gcaccagctc ctcccaacca aaaaccaaat tgattcattg
    61321 cagcttgtgg atatatccat aataaactaa atatatatga taaaattaaa gcaaccactg
    61381 gtgatagtgt aattacacat ttttctcctg caaagaagct taaaaatgct ggaactttaa
    61441 ctttatatgt tttattgtaa gtccatgcta tacaagctcc aacaattatc ccaccaaata
    61501 cacctgcatt taatgtatca ttcaataact ctaaatttga cttaaatatc atatatgcaa
    61561 ctacggctgc aaccattaca cttgtcttgt ctttagactt tgagtaggca ctagctgctc
    61621 ctaataaaaa taagtagtcc acatttgaaa atactatatt agatatatta gatatagctg
    61681 gcacattgag catatttgga ccaaataata atcctaatat acctgctgct ggcattacag
    61741 ctattggcac cagcatagtc tttcctaaaa gtgacagttg ttgcatcatt ttgtttaaga
    61801 atctcttcat atccaatccc ctttctattt cggtttaatt ttttgtgaaa acgatttatt
    61861 cacattttat attttaaata ttatatttgt ttaaacaaat tgtcaatatt ttttattcat
    61921 tttattttta tcatttagtt taacatataa atccttttaa gtgtattgct atatctagtc
    61981 tattttttta attcattttt tttattttaa aaatatgttt aaccatgtat ttgaaacaac
    62041 ccacaaaata attctatttt gtccttttta tggttgatat gattctattt tcactataca
    62101 tgtctaaaca catttgatat aatttgttca tatcagttca ataatattac taaagacctt
    62161 taaggaggga tagaggtata tggctaaaaa taaattttca gagatatatg aagtattgaa
    62221 agaggaaata cttgatggta aatatacaag taatatgatg ctgcctactg aacttcaact
    62281 tattgaaagg ttttcatgta gcagaaacac tgtacgtaga gcaatttctc aattaaacac
    62341 agaaggatat gttcaaagta taaaaggcaa gggtgttgta gtgttagaaa atagttgttc
    62401 taatgatttt tttctaaata tgcacaattt caaaggtgta gaatcgattg tagaagataa
    62461 aaaggttaat actgctacta gtgttctcca cttcagcaaa atcttaattg ataataagtt
    62521 atctaaaaag acaggtttta aagttggtag tgaagtatat taccttcaca gactaagata
    62581 tattgataac atacctaaaa ttctcgacat taactatttt ctatgtagta tagttaaaga
    62641 ccttgatgtt tctatagcac aaggctctat ttataaatat atagaagagt gtctaggtac
    62701 aaaaattgtt tcatccagaa agatatttaa aattgaaaaa gctacagagc ttgagttaaa
    62761 aacgctacct ctaaatgatt ataattgtgt tggtgttatt aaaaatagtg tatatactga
    62821 tgatggaaag ctttttgaat atactgaatc aaagcataca cctgaaacat ttgtattcat
    62881 ggatgttact caaagatatt aaaaaaatta tgtttaatta aacataattt ttttacgttt
    62941 agacacttta tcccatacta acctcactat tctatataca attatatgtt tatacaaata
    63001 attatttatt gtatatttta gctttaatat tagtttaacg cttgacaatt tgtttaaaca
    63061 tattataatt aacttataaa ttacaggagg tatcaagtat gaaaaattgg tggaaaaaag
    63121 caactgtcta tcaaatatat ccaaaaagtt tcaaagatag caacaatgat ggtataggtg
    63181 atataaatgg tattatagaa aaattagatt atctgtattc tttaggtgtt gatttattat
    63241 ggttgactcc tatgtacgtt tctcctcaaa gagataatgg atatgatata gaagattatt
    63301 ataatataga cccaaaatat gggacaatga gtgactttga aaaactctta aaagaagctc
    63361 ataaaagaga cataaaaata atgatggata tggtcctaaa ccatacatct actgaacata
    63421 aatggtttaa agaatctaaa aaaagcaaag acaatccata tcgtgattat tatttctgga
    63481 aagatgctaa acctgatggt tctgttccta ataactggat atccagattt agtggaactg
    63541 cttggaaata tgatgaaacc actaaccaat attatttaca tttatttgaa gaaactcaag
    63601 ctgatttaaa ttgggaaaat gaaaaggttc gtgaagaatg ttacaaaata ctagaatttt
    63661 gggctgataa aggtattgat ggttttcgtt tagatgtagt caacctactc tccaaaacac
    63721 ctggattacc tgatgaccct ataacaggac caaaaggaga tggaagaact cactacgctg
    63781 atggtcctcg tatacacgaa tatttacata atatgaatca aaaagttttc aaacctaaaa
    63841 atatagttac tgtaggtgaa atgtcatcta ctacacctga agagtgtatc aactatacta
    63901 gagaaaatcg agaagaacta tctatggttt ttagcttcca tcatatgaaa acagattata
    63961 aaggaggcgc aaaatggact aatgaaatgt tctctctgga taaattaaaa aaagcacaat
    64021 ctgactttca atataaaatg tatgaaggaa agggatggaa tgctctattt tactctaatc
    64081 acgaccaacc tagagcttta tctcgatttg gagatgatag attttttcgt caagaaagtg
    64141 ctaagatgtt agccataaca ttatttggtc tacagggtac tacatacata tatcaaggtg
    64201 aagaaatagg tatgacaaat gcctacttta ctaaaataga tgaatatgat gatgctgaaa
    64261 gtaagaatgc atactatcat atgattaaat ctggtattga tgaaaaggag gcattgctta
    64321 tacttcaaca aaaatccaga gataatgccc gtactcctat gcaatgggat aatactattt
    64381 ataaggggtt ttctaatcat aaaccgtggt taaaagttaa taatgacaat attagtgtag
    64441 aaaatgagct taatgattct cgttcagtat tttatactta tcaaagatta atcaaatatc
    64501 gtaaacaata tgatattttt acagaaggta cttatagatt gttagatgaa aatcataaaa
    64561 atctatttgt ttatgaaaga gagtataaaa accaaaatct tcttgttata agtaatttta
    64621 ctcatgataa tgtagtatat aaattacctg aaacttattt gaataaatta aactacacaa
    64681 tacttgaaac caattacact agagatataa ttgaaaataa aatggaaata aaaccttacg
    64741 aaagtataat gatatattat aaataaagtt atttatgtaa aaatcggtat aacaccctgg
    64801 tgttatacca attttactaa attcaaagta atatttatag aatatctata gattacaata
    64861 attatttgac ttctacatca ttatatttct ttgaatatac tttagtcata tagaaatata
    64921 taggaactac aattatcatc ccacctaatc cccaaactat cttcataggt tcatcaatac
    64981 ctgcttttat cagtaataat atacttataa taactgcaaa tattggtata attgctcctc
    65041 caggtattct aaaatttaca tctttaccag catacttctt tctcaatatt agtacagcta
    65101 aacatgtagg aatatattga ataaatctag atacaacact tatttgagat aatgttgtga
    65161 atgaacccga ccatgctatt agtaaagttc ctatgacaga tactataatt gctatataag
    65221 gagctccatt tttattagtt tttcttatag acttaggcat taatccttgt tcaaccaaag
    65281 cagcagcaga ccttggtgta aatatggatg atgcaatatt tatacctcca acagatacta
    65341 tagttcctat tgtaattata tacccaccta taggacctaa catcttaatg gcagcatctg
    65401 caacaggagt agctgaccct gctaagccat tacccaacac ccctatactt attcccagta
    65461 taagcatata tattactgat actacaaaca ttaccattac caatgcttta ggtacatttt
    65521 tcttagggtt ctccatatct tttgctgcaa cagctaaaga ttcaaatcct gtaaaagcat
    65581 aaaataaagt tatagatact gctacaaaag ctgatgacgc actttgacct ttttgtatta
    65641 ttacaaatgg ggtaaaatta gaacccttta taaagaataa tcctacagct ataaataaaa
    65701 ctattggtaa taattttcct atagtcatga tattatttaa gtgcttagat tgtttcattc
    65761 ccataaaatt tataatccca agtcctacaa ctaatatacc aattattata tttttagtaa
    65821 gtggttctgc tgcttgtggc catacagatg atagtgttac agcaaagaaa tttgccatag
    65881 tagcccaagc aatcatacta actatccact tcataaatcc aacttcaaat ccaacaaaat
    65941 ctccaaatgc ctcttttgca taaagatagg gccctccatc agtatcaaac caactacctg
    66001 cttcagcaaa acataaagca atagaaacag ctaaaagcat acaaaaaact aatactaata
    66061 tacttgctgg acctatatct gtataagcat caccaggtaa cccgaatata cctgacccta
    66121 ttattgcatt gattcctaat agtacaatac tccaaaaacc taacttaatg cctttttcca
    66181 ttgttattcc ccccaaagcc aacttaatat atttatattt tttatttaga tgcttcttta
    66241 actattgcca taaatatttt tttcatatta tcataatctc tagtcatcat ttctggatgc
    66301 cattgtatcc ctattacaaa ctcacttcct tccttttcta tagcttcaat taaaccgtct
    66361 ttagatgtag ctgaaacaat atatcccggt gctactttgt tgacaactaa gtgatgaaaa
    66421 ctattaacta aaacttcttt ttctcctaat atttcatata attttgtccc ctcctttatc
    66481 tgtactgtat gtgtaggtac attagataga tgttgctgat tgtgtttaat ataagcacct
    66541 tcaattaaag ataaatcttg atacaaacta cctccttcag ttacatttat aatttgttct
    66601 cctctacata tacctaaaac tggttttttc atatctaaag catgttttaa taatttcata
    66661 tcaaaactat ctctctttgg tgatattgcc cccaacttat tatgaggttc ttctttccat
    66721 atgagtggat ttacatcttg tcccccagat agtataagcg catctatatt agatacctgc
    66781 tcctttataa tctcatcatc atatacaatc ggtacaatat atggtatagc tttacacata
    66841 actactgatt gtatataatc attatttaca taagcccttt catagccagg aaacattcct
    66901 ccttcatcaa ttagtaaatt acctgatatt cctattattg gatatttctt tttagccaat
    66961 ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc tacttctctt aaagagcaag
    67021 tagcatacca agattttatt tatttgaaca ttataattta ttttagaaat actttttttg
    67081 aatacgagta atataaataa ctttatttac ttatatttta ttttaatcct gtttagtttg
    67141 acttttttaa attgttttaa gtacaactaa taggtaaaat aggcatttag attttccttt
    67201 ttatcaacct attaaatact gtttaatata ttttttcccc ttctatttaa ttcacttccc
    67261 tttctaccag attcacatat tatcaaatcc agctctttta acatattaag tattcccctt
    67321 acctcacctt ctgttatatt gtagcctaat tcatttattg aattaacaat accatttcta
    67381 cctatatttt tattaagatt attggcattt tttaaacact ccattactaa ctttactttc
    67441 tctatagatg ttctatcctt taatatactt atttctttac taaaatcttc agttatattt
    67501 aataagttat gaggtaaatc atttattaaa ataacatcct cagaactcat aatgttaata
    67561 tacatagcag tattttttaa ttctcttata ttacctggcc attcatatgc attaataatg
    67621 tcttgaacat cttgactaat tgctctcttc cttttcataa aatgctttaa cataacttct
    67681 atatcttctt ttcgctctct taaaggtggt atatttatgg gcaatacatt tatcctatag
    67741 tataaatctg ctctaaatct atctcctttt atcatttcta ataaattttt atttgtagcc
    67801 gcaattactc taacatctat atctattaca ttttctcctc caaccctcat tacttgattc
    67861 tcttgtaata ctctaagtaa tttgctttgt aagtatatag gcatgtctcc tatttcatcc
    67921 aaaaatatag ttccattgtt agcctgttca aataatcctt ttttacctcc ttttaaagct
    67981 cctgtaaaag caccttcttc atatccaaaa agttcacttt caagtaaatt ttctggcata
    68041 gctgcacagt ttattgctat aaatggctga ctatgtcttg gtgatgcatt atgaatagac
    68101 tgagccatga gttcttttcc cgttccactt tctcccgtta taagaattgt ataatcagat
    68161 tcagctattt tcatacctaa tttcttagtt ctttccatct tcgttgaaac tgtttttata
    68221 tcatcaaaag tatatctagc aacttgtccc ttttctctaa gcttctttga taggttttgc
    68281 tccaattttc ttatgtaagt tatttcttga atacaaaaat aatatccagt aactcttcca
    68341 aaactctcta tattatactt atttacgttt aagtacttat tattaaattt tataacttca
    68401 tcatgtgcct tactcaaact aaaaatcttt ttaatattct cattctctaa ttcatctata
    68461 tttttcccta taatattact ttgaatatct agtatatccc ttaaactatt attgcaaata
    68521 attatgtttc tcttatctga taccattgcc atacctaagt ttgataaatt tattatagaa
    68581 tttagttcat ctgcttttat agtaagttct ttgtatttag tatttattcc tgaatataaa
    68641 ctaattattt catctgtata cttaattagc ctctcagtaa tttctttatc atccagtttg
    68701 agttttgcta ttatttgaat aaacgttgaa ctatcaatat acctatggcc taaatcaata
    68761 acttcttcta tatattttgg aactaaactt gactctcctg gcgttatagc tatcttaata
    68821 tttctatatt cttcattggg attgtatggt ataaaattca gatgatttat tcctatattg
    68881 tataaattag atattgtttc caatacagta tgcttattat cgtttactac taatacatct
    68941 attccttctg gcaatgaaaa taacttatat atatcctttt gctttatgct cctatttata
    69001 gttattatct ttgagacatc ttctacatat ttttttattt ttaatactct atcatctatc
    69061 attaccaata ttatgtcatc ttttattaat tccttctctg ttattttatt taaataataa
    69121 ttttttatca ttactttatc tttaaatatc atttctagat ttttatttaa gaaacattct
    69181 aatttagaca acccatcagt tactactgct attgttttca tttgattact ccttttaaaa
    69241 tatactctac tcttttataa tatacatctg attttcactt taatagcaca aaaaattgta
    69301 catttagtat actaacatat tcctttatta gttttatgtc cctaaatatc atagttgcaa
    69361 tatctatcat atttcctaaa cttctataga gcaaaaattg tgccaaatta ataattttct
    69421 taattttatt ctaaagttcc tcaatagtat ttacagactt agcacttata gcttataagt
    69481 ctttatatta ttaatctatt tttataggtt aattgtacaa atcatatatc acttttaccc
    69541 attaaatgca ttataaaaaa gatggaataa cgattcgtca ctccaccttt tttataataa
    69601 aaaattgtat atgtaaacac tatttactac tcttctaagt acaaacagta tctacatact
    69661 ataacctttc accattagat tctattactt gtttatacca gtcaaaactc tttttcttaa
    69721 tcctagacaa gtctccactt ccatcatcat acttatccac gtatataaac ccatatctct
    69781 tggccatctc accagtagat gcacttacta aatctataca tccccacgga gtatatccaa
    69841 ataaatcaac accatcttct acagcttcct tcatttgctc tatatgtgct tttaaatact
    69901 ctatacgata gtcatcattt atcactccat tagattcttt tttatcataa gctcctaaac
    69961 cattttcaac taccatcata ggtattctat atctatcata aatttcattt agtgcatatc
    70021 taagcccttc agggtctatt tgccatcccc attcactaga cttaagatat ggatttttca
    70081 ctccacctaa tatgttgcca cttgtattat cttctttaga atcactactt acacatgtag
    70141 acatataata actaaatgca taaaaatcta cacaaccttc ttttaaaatt tctaaatctc
    70201 ctttttccat ttttatttca atattatttt ctccaaaata tctattcata taagttggat
    70261 attcgccacg cacttgtaca tctccacaga aataattcat tactctacta tctctttgag
    70321 ttttaactgc atcatctgga ttacaagtat aaggatacat tgttgcaaat atttgcatac
    70381 atccaacttg ataatttgaa tctatttcat gagctaactt aacagcctta gcacttgcta
    70441 caaattgatg atgtaaccct tgaaaacgaa gttgaggtat atctacttgg tttataaaat
    70501 ctgtagtccc ttcatttaat attccttgtg ataagtagcc tcccataggc attgtagctg
    70561 aatttatttc attaaatgtc agccaatatt ttacttttcc tttatatctt ttaaatattg
    70621 tttcacagta attcatatac aagtctatta catctcttga aacccatcca ttgtatttct
    70681 ctgttattgc aaatggtatt tcatagtgag atattgttat taatggttct atattatatt
    70741 ttaaacattc atctatcaca tttttataaa atttaagtcc tgcttcattt ggttctttct
    70801 ccattccagt tggaaatatt ctagtccatg ctattgaaaa tctaaatacc ttaaatccca
    70861 tttctgcaaa taaagctata tcttctttgt atctgtgata aaaatctatt gcttcatgac
    70921 ttggataaaa tgttcctttt tctataactc tcgttattct tttacttact ttttgagaac
    70981 ctccagtaca catatctgaa caagaaggac ctttcccatc taaattccat gctccttcac
    71041 attgatttgc tgctgttgct ccaccccata aaaaatcttt tttaaatgac atacctatat
    71101 cctcctatta taattcttca ccatttgatt ctattacttt tttataccag gcaaaactct
    71161 tctttttact gcgttttaaa gttccatttc catcattatc tttatccaca tatataaatc
    71221 catagcgctt cttcatttct ccagtacttg cacttactaa gtcaatacat ccccatggtg
    71281 tgtaccccca taaatcaaca ccatctaact caacagcatc tatcatggct tttatatgag
    71341 cacgtaaata atcaattcta taatcatcat ttatatttcc attttcatct ggattatcta
    71401 ctgctcctag tccattttca actataaata atggtttttg ataacggtca tataaagagt
    71461 tcatagttat tctaagccct aatgggtcta tttgccatcc ccactcactt gcatctagat
    71521 atggattttt aagacttgca aaaacatttc cttctgttaa aatatttact tttgggtctg
    71581 cacttgtaag tcttgatgag taatatgaga acgaaataaa atctactgtg ttctctttaa
    71641 gtatcttttt atcatcttct tccatcacta tatttattcc ttttctctcc aattctttta
    71701 atgcatatgt tggatattct ccacgagatt gtacatctat aaaaaagtaa ttttccctat
    71761 ctttttctat agatttccat atatctagtg ggttacaagt attaggataa gtatttccag
    71821 ctgctaacat acaccctatt ttaaattctt gatttatgtt atgtgctagt ttagtaacta
    71881 acgcactcgc aactaattga tgatgtgcag cttggtattt tatttgttct tcattttctc
    71941 cctcttcaat atagagacct gcacctataa atggtaaatg aagtatcatg tttatttcat
    72001 taaatgttag ccaatacttt accttacctt taaaccttgt aaaaataact tcacagtatt
    72061 ttacatataa gtctactagc ttacgatttc tccatgctcc atattttttt accaaataca
    72121 tgggaacatc aaaatgacag attgttacta ctggttctat tccatacttt aaacactcat
    72181 caaataaatc ttcatagaat tttagtccct cttcatttgg aacatcctca actcctgttg
    72241 gaaatatcct agtccatgct attgacatac gatagcattt aaatcccatc tcagcaaata
    72301 attttatatc ttctttataa ttgtgataca tatctattgc ttcatgagct gggtaatagt
    72361 gttcatcatc acattcaaac attttcattt tcccagccaa aactggaaat ctatcttttc
    72421 cttgcggtgt aacgtcaaca ttagctagac ctctaccatc ttctttatat ccaccttcac
    72481 attgatttgc tgctgttgct ccaccccata aaaaatcttt tctaagtgcc ataattctac
    72541 ctccaataat ttatttatag taactttatt ttaaaataaa aatacatata tataattctt
    72601 aattaataga actctctttt acgctaaaac agttatcaat ttctgactat tatttacatg
    72661 cttttgtgta ttttctacaa tatccacata atcagaataa ttagtaacaa ctactggtgt
    72721 ttctaagcaa tatccttctt ttgaaatact ttctatgtca aaagttatta atgtctgacc
    72781 ttttttaatc ttatcccctt gattaacaaa agatttgaaa tatttacctt ccaactgaat
    72841 agtatttaac cctatatgta tcaatagttc acaaccatta tcagatataa ttcctattgc
    72901 atgttttgtt ggaaataaag tcattacagt cccatcaaat ggtgccacta cttttccttc
    72961 tttaggtgtt attgctacac cttttcccaa tatacctgtt gaaaacgctg catcttgtat
    73021 ctgctccaaa gcctttactt caccttttat tgggctatat actacttctc ttttgttttt
    73081 ttctataacg ccttgcccat tatcatcttt agaaatttct actatagcat catctttcca
    73141 aaaaaataat gttaaaacaa atcctactac aatagctact attattgcta ttatagttgc
    73201 ataaacacct ttcatatctc cagttgttgg atttatataa ttaggtatac caaatattcc
    73261 aagtccaccc attacataac tttttacatt cattatcccc attacagctc ctgccacacc
    73321 accacctaca cagctatata tgaatggtgt ttttcttggt aaagttattc catatattgc
    73381 aggctctgtt acaccacata tccctgaaat aactgcaggt atacacagct cttttatctt
    73441 cttatctttt aatttaaagt acattgctgc tactactgct gtttgtgcaa aagatggtac
    73501 aaatgctccc actaatattg tgtcaaaccc catagtcata aggtttgaca tagctattgg
    73561 tactaatccc caatgtagac caaatattac aagcacttgc cacaataaac cagttaatgc
    73621 accacataaa attggtgaaa agttaaataa agctaaaaat ccatttccaa ctatattaga
    73681 agcataggta gctataggcc ctataaccat aaatcccaat ggtaaagaaa ctaataaaac
    73741 tgccattggt acaaagaaat tctttactac atctggtatt attttagcaa acactttctc
    73801 caacttagct gctaataata ctattaaaat tactggtact actgttgaag tgtagttcat
    73861 tgctattact ggtaatccta agaaagttat atatactggt gattcaaata ttgttccagt
    73921 aaatagattg tataaaggtt gacctgtaga tgctagagta tctaattgaa tacctgaata
    73981 gcataaaact gcacctataa gcattccaat aaatggtttt aatttgaatt tcttagcagc
    74041 tgtatatcca agaattattg gcatatactg gaacattgca tctccaattg catttaacat
    74101 aatatatgca ccatcagttt ctccatacaa acccaatgct gtaaatactg cattaaatcc
    74161 ttttatcata ccagcagcac acagaactcc taatattggt tgaaaaactc ctgatattat
    74221 gtctataaac ttattaagta atccttttgg cgcatctgag tctgaattat ctattgtatc
    74281 tcctcctaat ccagccaaat cgcacacaac ttcataaaca tctggaacat gattaccaat
    74341 aactacttgg tattgacctg cacttttcat tacagtaact ataccatcca ttttttttag
    74401 cacatcatca tttgctattg actcatcttt taatttaaaa cgtagcctcg ttatacagtg
    74461 agataaactg ctaatattat cctttcctcc tacattcgca atgatttctt ttgatagttg
    74521 ttcatacttt ctcatttttt ctcctccatt ttctttttga aatatgaagg tttggccaac
    74581 ttaatgtcac cacccaatat atataaacct aattattagg atttatatct gacttaatac
    74641 ttttacttac cactctttca acgtgaatag ttaagtataa tttttcctca tctgttaaat
    74701 cgtagttgta ttttttatgt ataaattcat ccaacttttc tatgaattta taagcatttt
    74761 tatatttaac ttttattaca tttaataaat catcttcatt attttcatgt aaattattac
    74821 taacaattcg ttctgcaaaa tactttaagt gagtaataaa tctatgataa taaactgaat
    74881 cttcatcaaa tactattctg taatggtatt taaccatatt tgaaatttct tgtattattt
    74941 gtgtaatctc atatatagtt tttatacttt gatccatttg tgcattaact atatgcagtg
    75001 ctataaatcc agcttcatct tctgatagtc taatttcaaa ttttttttct atataatcca
    75061 atgcctctaa ccctatttta aattcactct tataaaatct ttttatatcc caaagtaata
    75121 cattttttac acttactcct tcacgagctc tttctattgc agtatacata tggtctgtta
    75181 aagaaatata aatactatca tttaatcttt tccctagttt ttctttagca tataaaatta
    75241 tttcatctga taattccata tatcttatag gaatatctgc tattagttct ttaaatttat
    75301 cagatatatt tgggtctgaa agtttgaata tcttatcaat tttatccttt gatatatgct
    75361 ctcctgtacg tcttttataa gctaaacctc tccccataac tactatttcc ttatgatttt
    75421 catcagttgt aataattaca ttattattga gtattttttc aataatcatt gtgatgcccc
    75481 ctttattagc attaagttaa aataaatctt ttttaattat ttagaatatt taaaatgtaa
    75541 tttattacat aaaaaaaaac ctatttatta cacattaaat agactcccat gagtcctgag
    75601 taataaatag gtttagcttg tatttaaaca atcactatcc gtttgtattt tattttaatt
    75661 attatttatt atatgttagc aaatttattt tttagtgtca actttttctc aatattttaa
    75721 ttttaaataa attatataaa ttgaatcttc aaagtactat ttttatcaat aaacaaaata
    75781 aaaaaggtat tactttcttt aagtaatacc catttatttt taatttaagt ggtttttctt
    75841 aatctcatca agtagtccat cttctaaata aatttctaat gcacatccta ccattgcctt
    75901 tactgctttg tgaagtttat cataagcttc ctcagaattt gcataattta gaaattcttt
    75961 ttcatgaaca tataccattg gacttatatc taaggctatt tctgtataca tagtagggca
    76021 aacttgactt acattaccta tatctgtaga acctactgga ccatctttac cttctaatat
    76081 atctgttata cctgcctcaa tcaaattatt ctttgttatc ttttgaagta ctttgttatt
    76141 tattatgttg tcaaaagaat tttcaaagta tctataagaa agcttagctc ctgtcattaa
    76201 ctctgcacct tttgcacagt ttataacttt tttagttact tcatctaaat aacttctttc
    76261 agcagctctt acgaaaaact tacaagctgc tttttctgga actatatttg gagcctctcc
    76321 tccatttgaa actataccat gtattctaac atctgatttt acatgttgtc ttagtgcatt
    76381 tatcccagca aaagtaagtt gtactgcatc tagtgcattg actcccatat gagggtgact
    76441 agctgcatgt gctgataccc cttcaaaact aaactctaat gaatctatcg ccaaagcctt
    76501 acagtttata ttattaaaag cttctatatg catttgataa cttacatcaa catcatcaaa
    76561 agctcctgcc ttaactaaat cacattttcc tcctgttgtt tcttcagctg gtgttcctat
    76621 tagtacaatt tttcctttaa aattttcttt taacttactt aatactatac aagctcctag
    76681 tgtagatgct gcaatccagt tatgtccaca agcatgagct ggttctttat tttctccata
    76741 tccaggaagt gcatcatact cagctaaaaa tgctatggta ggtccatcat tatctcctaa
    76801 ttcagctcta aaagcagtct ccatattaca atatggataa actgtatcaa atccatattc
    76861 cttcattttt tctactaagt attttgatga tatgtattct tcttccccta attcaggatt
    76921 gttaaatata aaattacaaa cttcttcaat ttcttttcca agttcattct ctatctcata
    76981 agctctcttc tttatactct ctatcataat ttacctccta ctagaatcct agatataatc
    77041 ctattgtcaa aaatgccatt acaagaattg ttaatactac cattaaagga attacgaatc
    77101 taacccactt tgaccaagga atattggcta ctgctagtgc tcccattaaa acacctgatg
    77161 taggtgttat caagttagtt acacctatac caaattgata agctgtaact atcatttgtc
    77221 ttgatacatc tactaagtcg ccaagtggtg caagtacagg tattgtaagt gatgcaagcc
    77281 ctgatgctga tggtattaaa aatgctatta atccttctat taagaaagtt aaatttataa
    77341 atatagtttt tggtaatcca tttaataaat ttgctgcaga atttaaaatt gtatctatta
    77401 taaatccatt ttctgctatt attacgattc cacgtgcaaa tgctatacac aatcctgctg
    77461 taattaaatc tcctgctcca cttataaatg aattaactat ctcatcttgt ttaagccctg
    77521 atgctattcc tgatattata cctatagctg taaatatcat tgcaatttca gttatgtacc
    77581 attctagtct taaaactcca tatatcataa tcgccatacc tattgtgaaa atggctatta
    77641 ccatggcttc tctttttgta aattccttta tatttgtatc actattaacc attaattctt
    77701 gatttagtga tatatcatgt actaaagatt ttttagggtc ttttttaact ttattggcat
    77761 acatcataac aaatcctata gatatagcca tatatattat aaacataaca actctaaact
    77821 ctattccaga acctggtact atttgtgata atgcttgtgc tattcctaca gagaaaggat
    77881 tcgtagtagc tgctccaaat ccagctgttg ctcctataaa tactgtggcc actgctgtca
    77941 atgaatcata tcccatactg gtcataagtg gaataaatat catataaaaa ggtagtgtct
    78001 cttcactcat tccaaacgtc gtcccaccta gtccaaatag aatcattgaa atcggtatta
    78061 ttaaaaattg aaacttcttc attttattta cagtatgagc tataacagct tctatagctc
    78121 ctgttttatt aactatccca aaagctcctc caactattaa aacaaatcca actacttcag
    78181 ctgcatctat aaacccgttt ataggtgctg taaatacatc agtaattcct tgtggatttg
    78241 atggtgcttt ttcataagtt ccagctacaa caactgacct tccgtctata tctactcttt
    78301 caaaatttcc acttggcact atccaagtaa gaattgccat taacactatt agcaaaaata
    78361 ttattgtaag tgtctgtggc atagtaaact ttttcttttt taaactgctc atttttaatc
    78421 tccccacttt ttaaattaaa tattttatta ttgcatccct ttatcctatt ttgtatagat
    78481 gtaaaaaagc tcaacatgga tacatgttga gctaaatgtg ctaaatacaa catacctgca
    78541 tcttcccttt atcttgttta agatagctct ccatcaaaat tcgggataat tttgatgaca
    78601 gtactgtacc tattaaatac agtcccagca ctagcttcgg acactagcac ttcggcactt
    78661 actcctttta tattgcctca ttggtttcca acctcttcaa tattaatctt agtaaatgca
    78721 acctctacct caatctgttt gttagatgag gtaattctta ttaattttta ttaattcaaa
    78781 gtaattgtag cataatatgt cgagattgta aatattttca gtataataat tcttacttta
    78841 tgatttcatt ccatttttca caaataattt tttctccata taatttaaat actgtagtat
    78901 attttaaata cacacaaact ttccacaaat tttaaattat tataatacct ttataataaa
    78961 aaataaaagc tatgtaaact tatcaattta cacagcttta tatacattaa tctactccaa
    79021 gtacagcttt aagctttgat gctattgtaa taactcgtgg accaaatata acttgaactg
    79081 agtttttctt tttaacaact ccacttgcac ccaattcatt tacccagaag ccatcttcat
    79141 agaatccatc aattgaattt agctctaccc taagtctagt tgcacagttt gtaacatttt
    79201 ttatattatt acttcctcct aaaccttcta ttatagaatg tacaagcttt gtatctttat
    79261 catctacttc ctcttctact gcattgttgt atgctgtttt accagcatct acagattgtc
    79321 tttctctata ttctttctta ctataaagct taacttcttc tgaacttctt cctggtgttt
    79381 ttatatcaaa cttcttgatt gcccatgtaa atattatata agtaactata aaaaatattg
    79441 gtatcaaaaa taataatggt aacgcatcca ctttttgtgg ttgtaataaa ttaggaaaca
    79501 tatttctaaa tgattctccc attatagaaa cattaaacat ttctgtaaat acatacgtca
    79561 atcctgccag tggtacatat gctacaaaat acaataatgg tgcaacaaat aggaatgtat
    79621 actctattgg ttccgttacc cctaccaaca tggctgttac tattgctggt atcaatatac
    79681 ttattgcttt tttcttatta gcattatcag ccaacttata catagctaaa gctgctgctg
    79741 gtaatattgc aaaatgtatt aataaacgac ctgttgtgaa gttacgagtt atataagata
    79801 gtgtatctgg gtctcccatt tgagctaatc ttatattatt aacaccctcg taaattgctc
    79861 cacctacttc cattgttcca cctactcttg tatattctat agggaatgca attaagtgat
    79921 gtataccaaa tggtaataat gctttatcca ttgctccaaa agtaaatgtt cctaataatc
    79981 cactagaacc tatccaatta ccaagtttag ataatgccaa ccctactggt ggccatataa
    80041 agtacattgc tgtacccaat actattgatg ctataaacat cataatcatt gaaaatcttg
    80101 gacctgagaa aaaagataat gcactaggaa gttctatgtc aacaaatttg ttatgtactg
    80161 cactagctac aaatccagct attatagctc caaatatact acaatcatat gaaaagattc
    80221 ctaagaaaga accatataaa gaacttcgac tatatgcttc tgcctctcct aatccagatg
    80281 ccataaacgc ttctgctgta actgtatctg aatttatacc atttgctgct aacaatgtac
    80341 ttataactgt gtgcattcct ataaataaaa caattcctcc taatgcaccc catccttttt
    80401 cctttttaga taatccaaag gctatacctg taacaaagaa tattggtaag tatctcatta
    80461 ccataaatcc taaatcgttt attactttaa agaatacatt tataaatgac ccttctttta
    80521 ttagtccgct aagccctaat gcagaaacgt tttccatatt tgcaaaagct gaccctaatc
    80581 ctataaatac tcctgctaca acaagtaata taacaggtat catcattgct ccagaaagta
    80641 cttgtaattt atccttcata gtattatttc ctccattttt attgaaatgc caggctatat
    80701 aatgcatata tatatacttc tattgctttg tataatgtat ctatacttat attttcattt
    80761 gcctgatgtg aatttggatt atccccctct attgtgggtc caaatgcaac tccatttttt
    80821 aacactcttg cataagatgc agctcctttt gtaaaaagtt cggcttcttt tccaaaacct
    80881 tgtttatatg catttgataa tttatttatt aattcactat ctggttttac atacagtaat
    80941 tttaaatcct tatatatatc aactattaaa ttttcttttt ttccaatttc attaattcta
    81001 ttagttataa actcccaact tataccctta ggatatctaa agtctatttt catattcaat
    81061 tcatttttct ctaagaagat actcattata catattgtag aaactcccat atctacatct
    81121 tctttataca tccctaattt ttttccatga ttatcatctg taaagtatga atttaaaata
    81181 tcccttatta taacgccttt atcatttagt tttttaattt tctctaaatc ttttgctaaa
    81241 ctaaatgcac agttatatga cctgtgtgga tttctcgata aagccctttc tcctgtatat
    81301 cttactaata ctttaccttc atctaattct tctatctctg tataataagc taatgattct
    81361 actaaatctt tcttatcatt agttttaaaa acagcctcaa ttttgtcaca tacaaatcca
    81421 tcttctttgt ttgactttat atcaactaaa ttgtgatttc taaatttatc tttatctatt
    81481 ttctttctta gattgaagta taaaatacct ttttctccat ttactattgg gaagtttcca
    81541 tctggagaaa atgcaaattt tggctgttca ttgacactaa agtaatgctc catacattcc
    81601 caagtcgtct cttcagctcc acccactatc aatctaatct ttctcttcgg cttttcatta
    81661 agttctctta agaaaagtaa tgcatataaa atccctatca aaggtccttt gttgtcatta
    81721 actcctctac cataaagata gttatctttt tgacatactt taaaaggttt actcttccaa
    81781 agttctttat tatatattgg caccacatct atatgattaa gcacccctac tacttcttct
    81841 ccttctccat attctatatg aattgcatat ccatcaaagt cttttacaac aaaatctttg
    81901 tcttttgcta tttgaataag cttatcgaag gcatttctta tttctatccc aaatggttga
    81961 tttatatctg ttgtactttc atctctaatt gaaggtattt caactaaact tctaatatca
    82021 tttatgatat tttcttgatt ggattttaaa taatcaataa ttagttcttt gtttactatc
    82081 atcttaatta tttatcaccc ctttatatta attttacaaa gcatttcagt tcatatctac
    82141 attataaatt ttgaatatta ttttttcaat atttcaaata cttctctaag ttaccataat
    82201 gatacggtaa acaatcaaca gattatgaca aaatcatact atacctatta ctaatatttt
    82261 aacttatcta ttacgtcttt tttaatgtaa gtttattcca aaaatacata aaaaaagatg
    82321 tagccttagc tacatcttct tatatctatt caacttccat tattatgcta ttaatatcta
    82381 catttccagg ttctatccta tcttttactg ttctcttaac agaatcagca tcatccatta
    82441 taactacagg agttattata tttaaatcat ttgacgttat aaaatctaaa tctacttcca
    82501 ttattaaatc tttagctttt accttatctc ctgcctttac cttaacatca aacggctttc
    82561 cttctagtga cattgtctct aaacctacat gtattaatat atttgtatta tcattactct
    82621 taatcattat tgcatgcttt gtatcaaata cattgactat ttcaccatca acaggagaat
    82681 ataacttacc ttcacttggt attattgcta ctccatctcc catcatcttt tgggcaaaaa
    82741 ctgcatctgg aacatcttct aagttcacta aatttccctt aaaagggcta tatattttct
    82801 caatcttatt ttcttttttc ttaaatatat taaacataac acactctcca cattataaaa
    82861 ttttatgata atatttttct taaagctttt gctacaccat catttacatt agtatcagtt
    82921 acaaaatctg ccatagattt tacttcatct gtagcatttc ccatagcaat accaagacct
    82981 gcaaatttaa tcatggatat atcattttca ttatcaccta tacaaataac ttctttgcta
    83041 tctataccta atatatcaca aaatttttta actgcaatac cttttgaagt acctttactc
    83101 ataacttcaa agtttatctt tccagaactt actacttcta aatcttcttg ttttttaaat
    83161 tcttcctttg cttctctcaa agaatctacg ttttctgaga aaagtataca ttttaaaatt
    83221 tcattatcag ctttttctaa taaagtttca caacttgcat tttctattat ttcaattctg
    83281 tcttcttctt tatttttact atttattaat ctatatggat gttcttcacc tatttcattg
    83341 ttggatataa ctattttaca gcctttaaaa tgtttgttta aattatactt ttcacctatt
    83401 gtatagattt tctttagagc ttctttgctc aaaaccttct tatactcata tcccagtaat
    83461 ttaaaatatg tcccattagt tgatataata tatatatcat cccctaaaac acttgtaaaa
    83521 tatttcatag cattataagg tcttcccgta actaaggcta ccttgatacc cttttccatg
    83581 gcttctttta gtgctttaat attttcttct gatacttcaa atcctttacc catcaaagta
    83641 ccatccatat cagtacaaat taatttatat cgcatttgta tcacatccta gtaagatttt
    83701 tctaatattt caacaaggtc ttctttcttt ccaaaaggta aaaatcctaa aactggacag
    83761 cttgttattt gttctaattg ttcctttgtt acattaaact cagacatttt tgtcttaagc
    83821 cccactctct ttaaaaaact ttctatttca tttcctaaag ttttagctgc ttcatcatct
    83881 tccacttttt ctaattcttg gtttagtatt ctcgctactt tggcaaattt ctctatatta
    83941 tccttataga acttttctgc aaattttggg aacactatag ccaaagcctc accatgagga
    84001 atatgtgtaa ctcctcctat tatttcactt agtggatgtg gtgctgctgc tccagcattt
    84061 gccagtgaac ttcctcctaa tgtatctgct actgctaact ttgttctgta ttctatatta
    84121 tttttatctt ccattacttt aggtagatag ttaatgacta attccataga tttttcagac
    84181 attatctctg taaaatcatt agcatttgga tttatataac tttcaaatgc atgagtaaaa
    84241 gcatcaaatg ctgttgatgc agtaattctc tctggcagag ttaacataag ctctgggtca
    84301 actatacatt cttttgagaa acaattttga tgaaatattg tatttttctc gcttcctctc
    84361 gatattactg ctgcttgtgt aacttggctt cctgttccag aagtagttga tacagctatt
    84421 aatggtaatt ctttttcact taccttatcg tatattttaa atggatttgt atatgtacta
    84481 aacatataat cccaatctat tttttctaaa ccaaaagtca atgctaatac ttttgcagta
    84541 tctatacttg aacctcctcc aacagctaaa acaacgtcta cagattcttt tttagctaat
    84601 tcaaaccctt cgtttattaa ttctacagtt gggtttggct ctactctctg aacctcccat
    84661 gactaaagtc acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa
    84721 ttctccacag gc
//
LOCUS       DAMJYC010000006        76993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJYC010000006 DAMJYC010000000
VERSION     DAMJYC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.006"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(2..1393)
                     /locus_tag="PC472_000431"
     CDS             complement(2..1393)
                     /locus_tag="PC472_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2725797.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1404..2624)
                     /locus_tag="PC472_000432"
     CDS             complement(1404..2624)
                     /locus_tag="PC472_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2725798.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2638..3426)
                     /locus_tag="PC472_000433"
     CDS             complement(2638..3426)
                     /locus_tag="PC472_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2725799.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3443..4717)
                     /gene="larA"
                     /locus_tag="PC472_000434"
     CDS             complement(3443..4717)
                     /gene="larA"
                     /locus_tag="PC472_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2725800.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4835..5548)
                     /locus_tag="PC472_000435"
     CDS             complement(4835..5548)
                     /locus_tag="PC472_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2725801.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5673..6932
                     /locus_tag="PC472_000436"
     CDS             5673..6932
                     /locus_tag="PC472_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2725802.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7212..7778
                     /locus_tag="PC472_000437"
     CDS             7212..7778
                     /locus_tag="PC472_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2725803.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7979..9184)
                     /locus_tag="PC472_000438"
     CDS             complement(7979..9184)
                     /locus_tag="PC472_000438"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2725804.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SVKKLLKID"
     regulatory      complement(9287..9393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9485..9853)
                     /locus_tag="PC472_000439"
     CDS             complement(9485..9853)
                     /locus_tag="PC472_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2725805.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9905..11182)
                     /gene="obgE"
                     /locus_tag="PC472_000440"
     CDS             complement(9905..11182)
                     /gene="obgE"
                     /locus_tag="PC472_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2725806.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11293..11583)
                     /gene="rpmA"
                     /locus_tag="PC472_000441"
     CDS             complement(11293..11583)
                     /gene="rpmA"
                     /locus_tag="PC472_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2725807.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11597..11914)
                     /locus_tag="PC472_000442"
     CDS             complement(11597..11914)
                     /locus_tag="PC472_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2725808.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11929..12240)
                     /gene="rplU"
                     /locus_tag="PC472_000443"
     CDS             complement(11929..12240)
                     /gene="rplU"
                     /locus_tag="PC472_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2725809.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12341..13762)
                     /locus_tag="PC472_000444"
     CDS             complement(12341..13762)
                     /locus_tag="PC472_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2725810.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13780..14481)
                     /locus_tag="PC472_000445"
     CDS             complement(13780..14481)
                     /locus_tag="PC472_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2725811.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14483..16327)
                     /locus_tag="PC472_000446"
     CDS             complement(14483..16327)
                     /locus_tag="PC472_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2725812.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16646..17839
                     /locus_tag="PC472_000447"
     CDS             16646..17839
                     /locus_tag="PC472_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2725813.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18051..19133)
                     /locus_tag="PC472_000448"
     CDS             complement(18051..19133)
                     /locus_tag="PC472_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2725814.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(19697..20488)
                     /locus_tag="PC472_000449"
     CDS             complement(19697..20488)
                     /locus_tag="PC472_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725815.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20492..20821)
                     /locus_tag="PC472_000450"
     CDS             complement(20492..20821)
                     /locus_tag="PC472_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725816.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(21306..21719)
                     /locus_tag="PC472_000451"
     CDS             complement(21306..21719)
                     /locus_tag="PC472_000451"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2725817.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21760..22890)
                     /gene="rodA"
                     /locus_tag="PC472_000452"
     CDS             complement(21760..22890)
                     /gene="rodA"
                     /locus_tag="PC472_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2725818.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(22975..23259)
                     /gene="minE"
                     /locus_tag="PC472_000453"
     CDS             complement(22975..23259)
                     /gene="minE"
                     /locus_tag="PC472_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2725819.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(23277..24074)
                     /gene="minD"
                     /locus_tag="PC472_000454"
     CDS             complement(23277..24074)
                     /gene="minD"
                     /locus_tag="PC472_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2725820.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24098..24781)
                     /locus_tag="PC472_000455"
     CDS             complement(24098..24781)
                     /locus_tag="PC472_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2725821.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(24879..27857)
                     /locus_tag="PC472_000456"
     CDS             complement(24879..27857)
                     /locus_tag="PC472_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2725822.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(27869..28357)
                     /gene="mreD"
                     /locus_tag="PC472_000457"
     CDS             complement(27869..28357)
                     /gene="mreD"
                     /locus_tag="PC472_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2725823.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(28372..29265)
                     /gene="mreC"
                     /locus_tag="PC472_000458"
     CDS             complement(28372..29265)
                     /gene="mreC"
                     /locus_tag="PC472_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2725824.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(29275..30285)
                     /locus_tag="PC472_000459"
     CDS             complement(29275..30285)
                     /locus_tag="PC472_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2725825.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(30406..31065)
                     /gene="radC"
                     /locus_tag="PC472_000460"
     CDS             complement(30406..31065)
                     /gene="radC"
                     /locus_tag="PC472_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2725826.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31110..31694)
                     /gene="maf"
                     /locus_tag="PC472_000461"
     CDS             complement(31110..31694)
                     /gene="maf"
                     /locus_tag="PC472_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2725827.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(31914..32885)
                     /locus_tag="PC472_000462"
     CDS             complement(31914..32885)
                     /locus_tag="PC472_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2725828.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(32901..33476)
                     /gene="rsxA"
                     /locus_tag="PC472_000463"
     CDS             complement(32901..33476)
                     /gene="rsxA"
                     /locus_tag="PC472_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2725829.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(33491..34084)
                     /locus_tag="PC472_000464"
     CDS             complement(33491..34084)
                     /locus_tag="PC472_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2725830.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34097..34666)
                     /locus_tag="PC472_000465"
     CDS             complement(34097..34666)
                     /locus_tag="PC472_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2725831.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(34666..35643)
                     /locus_tag="PC472_000466"
     CDS             complement(34666..35643)
                     /locus_tag="PC472_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2725832.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(35666..37003)
                     /gene="rsxC"
                     /locus_tag="PC472_000467"
     CDS             complement(35666..37003)
                     /gene="rsxC"
                     /locus_tag="PC472_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2725833.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQVPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(37219..38163)
                     /locus_tag="PC472_000468"
     CDS             complement(37219..38163)
                     /locus_tag="PC472_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725834.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(38274..39548)
                     /locus_tag="PC472_000469"
     CDS             complement(38274..39548)
                     /locus_tag="PC472_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2725835.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(39680..40051)
                     /locus_tag="PC472_000470"
     CDS             complement(39680..40051)
                     /locus_tag="PC472_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2725836.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(40279..40878)
                     /locus_tag="PC472_000471"
     CDS             complement(40279..40878)
                     /locus_tag="PC472_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725837.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKQLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41079..41291)
                     /locus_tag="PC472_000472"
     CDS             complement(41079..41291)
                     /locus_tag="PC472_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2725838.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(41364..43079)
                     /locus_tag="PC472_000473"
     CDS             complement(41364..43079)
                     /locus_tag="PC472_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2725839.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43079..43633)
                     /locus_tag="PC472_000474"
     CDS             complement(43079..43633)
                     /locus_tag="PC472_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2725840.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(43626..44228)
                     /locus_tag="PC472_000475"
     CDS             complement(43626..44228)
                     /locus_tag="PC472_000475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2725841.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(44237..46885)
                     /gene="polA"
                     /locus_tag="PC472_000476"
     CDS             complement(44237..46885)
                     /gene="polA"
                     /locus_tag="PC472_000476"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2725842.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47037..47124
                     /locus_tag="PC472_000477"
     tRNA            47037..47124
                     /locus_tag="PC472_000477"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47071..47073,aa:Leu,seq:caa)
     gene            complement(47311..47754)
                     /locus_tag="PC472_000478"
     CDS             complement(47311..47754)
                     /locus_tag="PC472_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725843.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(47757..47954)
                     /locus_tag="PC472_000479"
     CDS             complement(47757..47954)
                     /locus_tag="PC472_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725844.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(48146..49012)
                     /locus_tag="PC472_000480"
     CDS             complement(48146..49012)
                     /locus_tag="PC472_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2725845.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(49238..50107)
                     /locus_tag="PC472_000481"
     CDS             complement(49238..50107)
                     /locus_tag="PC472_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2725846.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(50613..50834)
                     /locus_tag="PC472_000482"
     CDS             complement(50613..50834)
                     /locus_tag="PC472_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725847.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(51335..52315)
                     /locus_tag="PC472_000483"
     CDS             complement(51335..52315)
                     /locus_tag="PC472_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725848.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(52479..53351)
                     /locus_tag="PC472_000484"
     CDS             complement(52479..53351)
                     /locus_tag="PC472_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2725849.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(53515..54066)
                     /locus_tag="PC472_000485"
     CDS             complement(53515..54066)
                     /locus_tag="PC472_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2725850.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(54086..54988)
                     /locus_tag="PC472_000486"
     CDS             complement(54086..54988)
                     /locus_tag="PC472_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2725851.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(55003..57375)
                     /locus_tag="PC472_000487"
     CDS             complement(55003..57375)
                     /locus_tag="PC472_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2725852.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(57596..58285)
                     /locus_tag="PC472_000488"
     CDS             complement(57596..58285)
                     /locus_tag="PC472_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725853.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(58526..59903)
                     /locus_tag="PC472_000489"
                     /pseudo
     CDS             complement(58526..59903)
                     /locus_tag="PC472_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(59897..60610)
                     /gene="rgbR"
                     /locus_tag="PC472_000490"
     CDS             complement(59897..60610)
                     /gene="rgbR"
                     /locus_tag="PC472_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2725854.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(60860..61420)
                     /locus_tag="PC472_000491"
     CDS             complement(60860..61420)
                     /locus_tag="PC472_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2725855.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSVLFMNMFVFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYVVQLYRRLMYYLRLKKFK"
     gene            complement(61516..62319)
                     /gene="zupT"
                     /locus_tag="PC472_000492"
     CDS             complement(61516..62319)
                     /gene="zupT"
                     /locus_tag="PC472_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2725856.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(62470..63633)
                     /locus_tag="PC472_000493"
     CDS             complement(62470..63633)
                     /locus_tag="PC472_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2725857.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(63693..64880)
                     /locus_tag="PC472_000494"
     CDS             complement(63693..64880)
                     /locus_tag="PC472_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725858.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(65243..66943)
                     /locus_tag="PC472_000495"
     CDS             complement(65243..66943)
                     /locus_tag="PC472_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725859.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(67198..67473)
                     /locus_tag="PC472_000496"
     CDS             complement(67198..67473)
                     /locus_tag="PC472_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2725860.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(67592..68164)
                     /gene="mobB"
                     /locus_tag="PC472_000497"
     CDS             complement(67592..68164)
                     /gene="mobB"
                     /locus_tag="PC472_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2725861.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(68148..69329)
                     /locus_tag="PC472_000498"
     CDS             complement(68148..69329)
                     /locus_tag="PC472_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2725862.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(69540..70274)
                     /locus_tag="PC472_000499"
     CDS             complement(69540..70274)
                     /locus_tag="PC472_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725863.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            70636..71541
                     /locus_tag="PC472_000500"
     CDS             70636..71541
                     /locus_tag="PC472_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2725864.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(71701..72543)
                     /locus_tag="PC472_000501"
     CDS             complement(71701..72543)
                     /locus_tag="PC472_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2725865.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(72527..73285)
                     /locus_tag="PC472_000502"
     CDS             complement(72527..73285)
                     /locus_tag="PC472_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2725866.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     YTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(73302..73772)
                     /locus_tag="PC472_000503"
     CDS             complement(73302..73772)
                     /locus_tag="PC472_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2725867.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(73772..74515)
                     /locus_tag="PC472_000504"
     CDS             complement(73772..74515)
                     /locus_tag="PC472_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2725868.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(74519..74932)
                     /locus_tag="PC472_000505"
     CDS             complement(74519..74932)
                     /locus_tag="PC472_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2725869.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(74958..75542)
                     /locus_tag="PC472_000506"
                     /pseudo
     CDS             complement(74958..75542)
                     /locus_tag="PC472_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(75727..76110)
                     /locus_tag="PC472_000507"
     CDS             complement(75727..76110)
                     /locus_tag="PC472_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2725870.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            complement(76220..76597)
                     /locus_tag="PC472_000508"
     CDS             complement(76220..76597)
                     /locus_tag="PC472_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2725871.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYRWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     regulatory      76806..76901
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT        24270 a        14150 c         8158 g        30415 t
ORIGIN      
        1 tctaatatat tttccctgga tttaaaatat ttttagggtc aaaagctaac tttatatttt
       61 taattaacat catataagca tctgaatttg attcatgtaa gtactctttt ttagcatatc
      121 ctatgccatg ttcaccagaa acttgaccac taagttctct agatttttta tacatatagt
      181 caaaagtttc ttttaatctt attttccatg tattatcatc cataccatct tttaaaatat
      241 atatgtgtag atttccatct cctgcatgac caaaactttt tattcttatt tttaatttgt
      301 cttgaagctc gtgagtgtat cttataaact ctgctatctt atctcttgga acaacgacat
      361 cacattcatc catttgtgtc gtagatgcct ttatagcttc taaaaatgca cctctggctg
      421 accatataga atcgtttctt tcttgtgtat ctgatataaa tacatctaat gcaccatttt
      481 ctaaacataa attagctact ttttcatatt ctttttctat atcttcagtg ctatttccat
      541 caaatgtaag taaaagataa gcatctgatg tattatctgg aaacttttta cctaaaaatt
      601 cttctgctgc caatattaca tctctttcca taaattctat agccgttggt attgattttg
      661 attttattat tttaggaaca gtttctattg ccattgataa atctggaaat ggtataagca
      721 aacttatact tttctttggt aaaggaagca attttaatat agctttagta actattccta
      781 aagtaccttc tgagcccact aataaatcct ttatactata tccagaacta tttttaacta
      841 cctttccacc tacatttata atttctccat ttggtagtac aacttcaaga cctcttacat
      901 aatcccttgt aacgccatat tttactgctc tcataccacc tgcattagtg tttatattgc
      961 ctgcaattgt agcacttttt tcacctgggt caggtgggta aaataaatct ctatcttgaa
     1021 catattgacc tattgtcatc aataaaacac ctggttctac tgtcaatgtt aaattttcat
     1081 aatcaacttc aagtatttta ttcattttac atagattaat cattattcca ccatttatgg
     1141 caacagcagc acctacaaga ccagtacctt gacctcttgg tgtgactggt atattatttt
     1201 tatatgcgta tttcattatt ttagatactt gttcagtttc aagtacattt actaaaactt
     1261 cagggtactt ctcaatccct ccaagttcat catgtgaaaa atcttcattt atatcacttc
     1321 caactaatac attttcttct ccacaagtat ctattaaaaa atctatatct tttttatcga
     1381 ttaatttata catttctatc cccctattta gctgctgatg ccatatattt ttgattatta
     1441 tctttgttat tttctatttt ttcaatcaat ttaggaataa tatcataaat atcacctatt
     1501 aaagctaaat gtgctacatc aagtataggc gctttatcat ctttatttat tgctactata
     1561 tagtcagcac cctgcatccc tgcaacaaat tgaacagctc cagatacacc acaagttatt
     1621 atcaatttag gttttacagt tctaccacta agacctattt gttttttagc atctatccaa
     1681 cctgcttcta ttacaggtct tgttcctgcc acttgagcgt ttaacttatc agctaattta
     1741 tatatcatct ccatatcttc ttgttttttt attgctctac ttgccacaac tatcacttca
     1801 gcttcttcca gaccaacttc tttatttttt tcttttacac tcagtacctt aattttagat
     1861 tttagttctt ttttttctac tttacataaa gttatctttc ctgtagtatt ctctattttt
     1921 tctggtgctg aaaatatttt atatcttaca gtggcaaatt gtggtctatt attaggtgtg
     1981 tttatatgag ccattatatt tccaccaaat gctggtctta tttggtctaa atcagtattt
     2041 gattgaatat caagtattgt acaatctgct gtaagacctg ttctaaatct agcggcaagt
     2101 cttggagcta atgacctacc taatgtagtt ccaccaacta atactatcgt tggttttatt
     2161 ttatctataa agttttctat tgcttttgaa tatggttcta tcctaaaatc tttaaattca
     2221 tcttcatcat atacaaatac ttcatccacc ccataagaaa tcaaattact gcacttatct
     2281 tttatgtctt ttcctacaaa tacacaatac acaggttgtt ttatttttga tgccatctca
     2341 cgagctttac ctataagctc gtacgttaca ggatgtatat ttccattatg atgttcaaca
     2401 tatactgcaa ttcccctcca ttcatcttta ttaatttgta ctttactact ctctataaat
     2461 tcaaatgcgc cttttggtcc acttttgaca cacaacttgc acatcttaca gccagcactt
     2521 atttctacta tacctgactt ttcttctata gccccaaaag gacatatttc taaaattctc
     2581 tttaagtcag ttattttatc ttgatttaca actatctttg acatttatta ctccccctta
     2641 caccaacttt aattcctcta atttatttac aagtgcaaaa cttagttcat ctgcatttcc
     2701 tcttacaata tttttatctg tatttggctt tggaggaaat attcttacaa cttgagtagg
     2761 agaaccatta agtccatagt tattttcatt tttatcttca aagtcatcta agctatatac
     2821 aggtatttct ctatctttag tattaagttt taatttaaat gaaggtagtc ttggttgaaa
     2881 aatgtctttt tctacagtta ttaaacatgg atattcaatc tcacatactt gtaaatcatt
     2941 tggcatatct atttcaacaa ctatactttc atctttaact tcaattaatt ttgtaacgtt
     3001 agtaacatga ggtatatcta aaaattcagc tacttcaggt cccacctgtg ctgtatcacc
     3061 atcagtagtt tgttttccac aaattattag gtctaccttt cccatcttct taataccttg
     3121 tgatatggta taggaagtag ccaatacatc tgctcctcca aatctcctat cacttattaa
     3181 tgctccttca tcagctccca tcataaaact ttcttcaatt accttctttg cctgaggagg
     3241 acccatgcta agtactttta gattcgcttt cttatcttcc tttattctaa gagctgtttc
     3301 taaagcatat aggtcatagg gattcatttt tgaatctact ccatctcttt ttagtactcc
     3361 tgtagtttca tcaacttcta ctgaagtcgt tccaggaact tgtttaatac atgtcaaaat
     3421 ttccataaag tacactcctt ttttatttaa ctataactga tactccatca ggtataacta
     3481 ctactttgct ctccttacca actatactat tcgccatttc taaagcttct ttaaagttaa
     3541 atgcatgttt catatgcatt ttttttatca tttcaggatt acacttatct gttaccatta
     3601 tcacagtata tttagataat attctcgcta gtatctgaaa ttcccattgg tctggaatcg
     3661 tatctactct atcgacactt ctaattttat ctaaaagttg ttttggagaa tctgcatttg
     3721 ctacattttc ataaaaagat tgacctccat gaccatcatt acatgctgaa atcattatta
     3781 ttactccacc gtctttacaa gtagcttcag ctgctgtcat tcctttaaca gattgataaa
     3841 tattttggtc tagtggatat ccaccatttg tagttacaac aatatcagat tttatagaat
     3901 ttacttttga taactctgtt acaaatctac atcctttagt atgtgctttt tcgacatcac
     3961 cagcaaagga agcaattatt tttttatctt tatctattac aacattcaat atgaatgcta
     4021 attttgcaac tttagctgca tataccatat cctcatgaat aggattatta ttcaattttc
     4081 cagttcttga attatcacta tctataaagt cactacagtg attgtacatt atagtctttg
     4141 cagatgcgat tcctggaagt acgctttttc tgccacctga aaaacctgca aagaaatgag
     4201 gttctataaa accttctgct atcaaaagtt ctgcctcata tgctagtttg tttatatata
     4261 aatctccacc tgaaggcaat atccctactt tacacataga tttatcatca gtagatatat
     4321 gcataactat atcttcatta tctactattt cttctcccat tttataaatt agctcttccc
     4381 ttgtacttgg cctatgaaat ccagttgcca ctataatctt cacatcaatt tctggattaa
     4441 cattcctaat ccttcttaat ataattggca tagttattct acttggtaca ggtcttgtat
     4501 ggtcacttgt aattatgact acattctttt tccccttaac cagttcttcc agagaattag
     4561 agcctatagg attattcata gattcttcaa ctatctcttc ttgagtctta tgttctatat
     4621 aattatccga ttttgactcc aaaacaccga gaaaacgttc atcaggtatt tctaaaatca
     4681 ttccttgttt tgaatacggt acttttaact ttaccatttt taatctccca ttataataaa
     4741 ctataaaatt ggtatgtggt cataccagtt ttacatttat ataatagtac cattatctac
     4801 catttgcaag aataatttat tttataacaa acttttaatt ttcatttttt ctataatttt
     4861 tacttataaa atccatatgt tcctttgctt tctcacatgc aagttctaca tcattttcta
     4921 caactgcatt gtatatagct tcatgctgtt taagcaatat attttcatta aaaggttctg
     4981 cgattatttt ttctcttgca tcttctataa atccatcaaa taattttgat gctgtcataa
     5041 aaagactttg aattaaataa tttttactta atgttgctag tttactgtga aatttttggt
     5101 ctaataatac cagtactcga ttattatctt ttttattagt ttctaccctc atttcatcta
     5161 ttatatgttt caattctata acttcagaag aagttattct ctttgaagca agttttgcaa
     5221 tctcaatttc taggattatt ctaagctcta atatatcttc aagtgtacca ttattaagtt
     5281 taaaaatcat agataaaggt tctattaaag ttttttctat atttgtacat ataaaatttc
     5341 cttcaccctg tctgctttct atcactccca tagtctctaa taccctaata gcttccctta
     5401 tggaagttct actcacattc atttgttcag acagttctct ttctgatagt aatttatccc
     5461 ccttttttaa ctctccattt agtattttat attgtatttg ctcaattact tgttcataaa
     5521 cttttttact agctatacta ttaaacattt tttcctccaa tatgataatc gttgttgtaa
     5581 tatatatcta ttatatatct taagccatta cattcatata tttttattac ttttaatata
     5641 atatcttata actcaaattt acaggtgata ttatgaaaaa aaataaaatt attgcttttt
     5701 ttgctccatc tatcatagtt ggagttctag ttttagggat tatcctatac ccaaatgaag
     5761 ctataaaatc tgcaagagaa ggcttttcta tatggtctaa tgttttagtt ccatcactac
     5821 taccatttat aattggagca aacttaattg ttgatttaaa aatagtggat attatagggt
     5881 ttatcatcaa tcccataact agatttgtat ttaatgtttc aggaaagagc gctttagctt
     5941 ttgctatatc tacagtatct ggttatccag taggagtaag tttagcttct gaatttagga
     6001 gtaatggaca aatttctaag tatgaagcac aaagacttgt gtctttttgt tctacatcag
     6061 gacctttatt cataataggt tcggttggta caggtatgtt ccaaaattca aacttaggtt
     6121 atttaatgat tctctgccat tatctaggaa ctatattggt tggtcttttc tttagaaatt
     6181 atggtaatga aaaccttcca aagacaaaat tggatttaaa aacaaatata aaaaatgtaa
     6241 tcaacattag aaattcaagt gggaatggtt tttttgtttt atttggaaat gctgtattta
     6301 atggagtaaa tacattactt acagttggtg gatttgtaat tgttttttca gtagtattta
     6361 aaatactatc tctttttaat gtaatatcat taatagcatc agttatatat ttgcctcttt
     6421 catttttagg tgttagtaaa gaactctgtc aagcatttgt aagtggtcta tttgaaatta
     6481 ctataggatg caataaagta tctagtataa caagttctcc agaaatttta agagcatctt
     6541 tagctagttt tttgatagga tttagtggat tatccatcct agctcaatgt tgtacatttc
     6601 tatcaaaaac ggacatagat ttaaaattat acatactaag taaatttgcg catggagttt
     6661 tagctgctat ctttatattt atactttatc caattgcaaa ctctgctgtg ttggtttcta
     6721 gctttgctga tacatacaac gttatatata ataatttaat ctggttttat tatcttagct
     6781 attatgatac aattcttcaa attgtagtaa taatttatct aatctcagcc gtatttattg
     6841 caaaaaaaca tatgaaaact ttatctacaa atgggaaact aatgcgatat aaaaatactt
     6901 ttttcactaa gaaattttca cgaaaaaaat aaaactaaaa ataaaactaa taaaacttaa
     6961 ctaaatactt ccattttttt attatatttg tcataatttt aataaccttt ttaattagtt
     7021 taacagcata gttatttcat cacaatttaa taattttatt agaaatattt ttcaaaaaat
     7081 tctccttaat cacatatatc acacttgtat aagtttactg ggaaattttt ccattatata
     7141 atatttcatt tatattatat aataaatacc gacctaaact taccaaatca aactaagagg
     7201 agtggttgaa aatggcaaca agacctatag aaatagacga atataaaaag ataatggaac
     7261 tattacatac tggctttact tatagtgaaa atggcgttga aaaaagattt agaaaaaatc
     7321 ctaaagttgc attggcacta atgttagaag ctaatttagg tctaagaata tcagatattt
     7381 taagattaaa gataggtaat tttaaaggta atatgttgga aataaatgaa aaaaagacgg
     7441 gcaaattaca gtatagacca gttaataaaa atataattga gtccataaat gaacatgcaa
     7501 gaaagtataa tcttaaatca aatgactact tagtaaatat aaagacaaag gctattcaaa
     7561 aacagttgag gataatttgt aaacacttaa atctgtataa tataagtaca catagcttta
     7621 gaaagctcta tgctactact caatttgaaa aaagtaacaa taatttagag ttggttaaag
     7681 aacttttaaa tcacagctct gtagccacta cacagcgata tataagggta actcagcaag
     7741 ctattaatga agctagtgaa aatttcttta ttggttaatt ataacgtata catttttcta
     7801 ttatcttatt aattcaccag atattaaaaa agagtgtctc aaaatgaact ttttagttca
     7861 tgagacactc tttttttata aaatcaaata tatttttatt atttcaacac tggaaaaaag
     7921 gcttatctct ctattttgag acaccctatt aaattttcat aattcttgca taaacatatt
     7981 aatctatctt caataatttt ttaactgatt cactgtaatt atttcttttt acaatttcca
     8041 aaactctatc aatctcatgt tctgactttg cacttacaaa aatcgccaat tcttcatcat
     8101 ctataatatc ataactatct acatatttat ctaaacattc taaaattcca tctttattat
     8161 tatacccacc aaatctaact actgcactac actcatcttc tataagtcca cttaaatcca
     8221 atttcccttt tgaaaaagac tttatatttg aacttctatt atctattata tctataatat
     8281 ctgcataaac agcacttcct gttgcacctc ttccagctcc cttacctaca aaagaaagtt
     8341 cacctactga atcacctact aaaataacta cgttgaactc attatctatc tttgaaagca
     8401 tattattatt gtccactaat gctggtctaa caaatccact tacttctcct gattcatact
     8461 tacttctagc aaccaatttt atcttacaat ttaatttttt tgcatattct atatcttttt
     8521 catctattga agttattcct tctaataata aatctttcca atatactatt tttccataag
     8581 ataaagtaga caatatagat aactttctag cactatcata tcctaaaaca tctgcatctg
     8641 gattaacttc tgcaaatccc aactcttgtg cctgttttaa agcgacatca tacggtaaat
     8701 tttcatcata catctttgat aatataaaat tacaagtccc atttaaaata gcgtttatgc
     8761 tctttatatt gttgccttcc agtgattcta tcaatggttt taaaactgga ataccacctg
     8821 ccacagatgc ttcaaacttg atgctaacat tatttaagtt agctaattct ataaggtcac
     8881 tacctttttc agccagtaaa tctttattag ctgtcactac atgtattttt ttatttaacg
     8941 ctcttgttac ataatcaaaa gctggattta atcctccaat tacttcaact agtatatcaa
     9001 tatccttgtc aaatatatct tctatatcag tagttatctt atcaaaatac ttcttatact
     9061 tgtgtttttc tttattttta actaaaattc caacaacttc tatatctctt tttgaagtat
     9121 tattttctat taaatcaatt aatccacttc ctactgttcc ataccctaat attgctatct
     9181 tcactgtaac atacccccat cattttgatt atattaaatt atttatatat ataaatatac
     9241 caataaaaaa agcttgagta taaactcaag ccatatgacg ggtttatact catctttcag
     9301 cataaatgct gcaggattta gcaccaatgt ataattaaat atactggttg ccgggcttca
     9361 cagggccagt ccctctgcca ctcttgataa gattgctaat ttaaatttat gtttaatagt
     9421 ataatacaat ttcttccaat tgtcactatt tttttaactt atttccctaa aaggatataa
     9481 actattatct aacagctctt ttagttggtt tacccttttt tgtaacatta ttatttttag
     9541 tctttgcttt cgcctgttca tgtcctggaa aaagtatttt aggctcttca atattcctct
     9601 tgtaaatagt aaatttaaaa ccaatagcct gcacaaattc agctcttaaa gcttcacata
     9661 ctgcattagc agtttcttta gtatcaagac ctgcattttc taatatagta acttttacta
     9721 attctctagt tcttaacata ccatctaatt gctctaagaa actctctgta acaccttctt
     9781 taccaatttg tgtagtaggc tttaaagtat ttgccaaaga tcttaaatac gccctttgtt
     9841 ttccttttaa catattctca cctctataat tctatactta aattatctat aatctatatt
     9901 taaattaatt gtaaaactca aattctaatt cataaatttt aaccacatct ccatcttcta
     9961 ttcccatttc tctaagccta tcaaaaacac cttgactctc catagctttt tggaaatatt
    10021 gaagggattc catatcatca aaatttacag aatacattat tcttctaagt gctttacctg
    10081 taactacata cacaccatct tcaatatcta tacttaactc ttcttcagta ccaatatcaa
    10141 gttctggtct atacatttct tcttctgaaa ctagctccac atcttctact tcttttagca
    10201 ttttagaaac ataggctatt acttcatcta tgccttgacg agttgctgct gacattttaa
    10261 atactgtata tcctctgcct tctaattcac tcttaaactt ctcaaactta ctctcatctt
    10321 ctaatatatc aaatttattt gcaactacaa cttgtggtct ctttgaaagc ttttcattgt
    10381 acaatttaag ctcatcattt atcttgtcaa aatcttcaat agggtctctt ccctctaatc
    10441 cagatatatc tacaatatgt atcaatacct ttgttctctc aacatgtctt aagaactcat
    10501 gacctaatcc tatgccttca gaagcaccct ctataattcc aggtatatct gcaagtacaa
    10561 aactatctcc aaacttagtt tgaacaacac ctagatttgg tgtaagtgtt gtaaaatgat
    10621 agtttgcaat tttgggttta gctttagtta caactgatag aaatgttgat ttacctacat
    10681 ttggaaatcc taaaagacca acatctgcta tcattttaag ttcaagagtt atccatcttt
    10741 cttctccatc tgtaccagat tttgcaaaag ctggagcttg tcttactgca tttgcaaagt
    10801 gttggttacc tttaccacct ataccacctt ttgcaactat agctgtatct ccttcttttt
    10861 ttaaatctgc taatacaaga cctgtagcct catctcttat tactgtacct gctggtactt
    10921 ttaatactaa attttctcca tttttaccag ctctttttcc tttgcttccg tcgccaccat
    10981 tttgagcttg atattttttc tggtacttaa agtccattag agttctaagc cccaaatcaa
    11041 cttcaaatat gatgcttgct ccacgaccac catctccacc gtctggacca ccagctggaa
    11101 cgtatttttc tcttctaaat gctaccgaac catttccacc atttccagcc ttaacaaata
    11161 ttcttgcctt atctataaac aaattcttca cctacttaat taaaaacttt taattagcat
    11221 taccaaaaat accattataa attcatcagt attataagaa aaaggagtgc ctcaacactc
    11281 cttttatgta tattactcag ctattgctac tggatatata ctaacttttt ttctcttctt
    11341 gtcttttctt tcgaatttaa cagtaccatc aactaatgca aataaagtat catcactacc
    11401 ttttccaaca ttagttcctg gatgtatttt agttcctctt tgtctaacta ttatacttcc
    11461 agcagttact aattgaccat cgaatctctt aacacctaat cttttagata tagaatctct
    11521 accattttta gaagaaccta cccctttttt agatgctaat aattgtaaat tcatgtttaa
    11581 catatggagt caccccctac ttttttaaaa tttttatatt ttttgggtat tcccgtttaa
    11641 tttctttaac cgctaattga aagtgattta gtaataattg tgcgtctttt acatgtgtat
    11701 ctttgactat acactctata tgaccttctt ctcctactac tttttcaaat tcaatctttt
    11761 gtaatttgtc taaagaattt ataactgcaa tagcattaga tgtaactgca gcacatacta
    11821 tatcatagcc tatttcagca aaatcagcat gtccctttaa acaaaaacct tgcaacaaga
    11881 aatcatcatt ataataatat ttaactttta tcataactta accaataact aagcgtttat
    11941 tttttctaca actatcttag tgtatgattg acgatgtcct tgttttcttc tgtaatcttt
    12001 ttttgcttta tacttgaaaa ctataacttt cttagcttta ccttgttcaa caactttagc
    12061 ttgaacagat gctccttcta ctactggaga acctaatttt aattctccat ctttagagca
    12121 agctaatact tcgtttaaag taacaacatc acctgcgtta gcttctaatt tttcaacgaa
    12181 taatacatca ccttcagaaa ctttatattg ctttccacca gtctttacta tagcgtacat
    12241 taaatacacc tcctcggcct caaactcgcc agtacaaggt acttcatttg aagatttaaa
    12301 cctttactgt gcggcattac aattattaat tataaaatat ttacatttta ttgtcaacta
    12361 ttttatcgaa aataacattg gttttattgt gctttaatgt actatttgcg tttaaagaaa
    12421 tctttatatt atatttttca ccaattttat caattatatc catataatat tcttttattt
    12481 gttcaaaaat aaaatcatta aattctacca ttatgttttt gtaaacagta tgttcactta
    12541 ttctcataat ttctttttca atactatcta gtatataatg tacagatttt attgtttgtt
    12601 caccatcaca acatggacaa gacattaaat aatacttatc aatggattct ttttctctac
    12661 gcctagcaac ttctactaaa ccaagttttg tcatacctaa tacttcagct tttcttttat
    12721 ctttattaaa ttcttcttct aggatgtgta aaatctcctt ttgatgcttc tttttatgca
    12781 tatcaataaa atcgattatt attatcccag ctatgtctct aattctaagc tgtcttacaa
    12841 tttcttttgc agcttcaaga ttagttttat aaacggtttc atcaagttta ccagttccaa
    12901 taaacttacc cgtatttaca tctataaccg taagagcttc tgttttttca attattaaat
    12961 aacctccact ctttaaccat acttttttat ttaataattt ttctatttta gattctattc
    13021 tgtataaatc aaatacatct ctattttcct caagacacag tttatcaaca taagtcttat
    13081 ctatacctct taaaatagac ttaagttcag agtacttatc ataactattg gtaattatag
    13141 attcaatatc atcattcaca ttatctttta catatttagt tgcaaaatct aaagacttat
    13201 ataataattt cggacctata ccaagtttat attcttttaa aatgttttca tactttaatt
    13261 tcaactcttc tatatctttc tttatttcat catgagtgca accttgagcc tcagtcctta
    13321 ttattaatcc tagattttcc ttatttactg ccttagtaat tttttttaat ctaaatcgtt
    13381 ctttttcatc agtaatttta tttgatattg tagttctatc attggaaggt atatagacaa
    13441 tataccttcc agaaatacta atctcagtgt ttaattttgc gcctttagtt cctatttctt
    13501 ctttatttat ctgaaccaaa atatcctgac cagattttat agtacttccc ttttttaatt
    13561 gtaaataagc aagtttctta aatccaatat ctacaaaaca agcttctatt cctggtataa
    13621 ctttttttac gattccacga tatatattgg atacagtctt cttatttaaa ttgtcttcaa
    13681 caaaaagttc tgttaatctt tcatcttcaa gtactgctgt tttctgggac cctattaggg
    13741 actcaataac tatctttttc acaacttcac ctctcgagct tacataggag tcactaattc
    13801 cccatcttct aatacatata aatctcttct caatatatca acatctaatg ggtcaatatc
    13861 aagttcaaac atctctaata tctttggtat aagtatatta gtatttaaat tagtttttga
    13921 gcctgtagct atagtagcag taagagtaat atgttcatct tctaaattta aaacatcaaa
    13981 ggttcttatc ataggtctta tatctacttc aaccatctta ccttttttat tcttttttgt
    14041 tatgattatt tctttattat tcataaagtc aattactttt ctttttacaa actctttcgt
    14101 cataggtctt ttcaaatcta tattaaacaa atattctcca tagtctatca cagaagaaag
    14161 agaaggtgtt tgtctatcaa tttcttttgc tttgataaaa tctatcccat ctggaagttg
    14221 aattgatact ttatttaaaa attcctctac tgataaatca tcttcttcta tttcaatatc
    14281 tacatactca ccttgacttt ctgtccctaa agccaaagca ttgccataac tcattttagg
    14341 gtgaggatta aatccttgag aatgtgataa gtttatttca gctcttctaa acgctctttg
    14401 taacaatctc tgaaggtcaa gatgtgatat atatatcatg tcgccttcct ttttaaattt
    14461 tactcttatt atcttactca tattagcaaa gcccccttcc tatatcatgt atatttattc
    14521 cacaaccatt gcagttatgt ctacaatcag aagtgatttt ttctttttta gcattttcgt
    14581 cttctctaat taagaatttt ttactaatac ctacatctat atggtcccaa ggaaatacct
    14641 cttcatagtt tctatttcta tgtgcataaa aatctataga aagattattc ttttccatag
    14701 cttctttcca tatatctaaa ttaaaatgtt ctgcccatcc atcaaattta gcacctagtc
    14761 taaatgcatc atatataact tttcctattc ttctatcgcc tctagctact actgcttcca
    14821 tcaaactagt ctttgaatca tgataattgt attttatatt gttatttctt aatttattta
    14881 ctaaatgtct ctgcttatta ataacttctt ctgtagtatc ttgaccatgc cattggaaag
    14941 gtgtaaatgg ctttggtaca aatgtagaag tactcactgt aacactaaaa cttctcttta
    15001 gctttcctcc atttactttt ctataaatat caataacttt ataagctaat ttagctatac
    15061 catctaaatc ttcataagtc tcagttggta aaccaatcat aaaatacaac ttaacactat
    15121 tccaccccat ctcaaaagct ctttctgttg cattttccaa atcttcttct gtaacacctt
    15181 tatttataac atctctaagt ctttgagttc ctgcttctgg agcaaaagta agtccagatt
    15241 ttcttacttg ttgtatttta tcagcaattt ccattgaaaa attatctaat ctaagggatg
    15301 gaagcgaaat cccaatatta ttagatgcat attcatctac caaataatca gtcaactctg
    15361 ataacttact ataatcactc gtactaagtg atgataatga tatttcatca tatccagtat
    15421 tcttaactaa tttatctaat atttctttaa gtcttttaac actcttttct cttattggcc
    15481 tgtatatcat acctgcttga cagaatctac atcctctagt acatcctcta aataattcta
    15541 gtactattct atcatgaaca gtatctatat aaggtactat tagtttttct ggataatcta
    15601 cagtctcaac atcttttatt atccttttag taggattttt agtaatccct tctctattag
    15661 gagttacact ttgaactgta ccatcttcat tgtacttgac atcataaaaa cttggtatgt
    15721 atactccttc tatgctagat attttatata aaaaatcttc tctagtcgtt ttatttttct
    15781 tccattcttt atactcattt actacttcta aattaacctc ttcaccttca cctaatatta
    15841 ctacatctac aaaatctgct aatggttcgg aattataagc acaaggacca ccaaccatta
    15901 taaatgggtc ttccaaagtt ctttcttctt ttaatattgg tatattggct aaatctaata
    15961 tatttaagat attagtgtat gaaagttcat attgaagtgt aaaagtgaca aaatcaaagt
    16021 ttgttatagg ctctcttgac tctaatgcaa ataatggtat acttttttct ctcattatat
    16081 tttccatatc aactgcagga gaatacactc tctcacaaaa tacatcttca tccttattta
    16141 ttacatcata taatatatga ctccctaagt gactcatacc aacttcatat aaatctggaa
    16201 agcaatgtgc atatcttatc aattcactat tactagtatc tttatgaatt gaatttattt
    16261 catttccaag atatctagca ggcttttcta ctttttttaa gatttttttc aagtctactt
    16321 tattcattta ttaaaattcc tccaaacaag ctctttattt ttattcttat ttataagtaa
    16381 tattatacac gataaaatat tttctatcaa aaatataact aaaatattga atttaaaata
    16441 catattattc acaaaataga atagctaaaa atatttaaga atattttcta ttaaattttt
    16501 attgaaatac aattttttca caaagtatga tatattataa acataaaact attgttttac
    16561 atgacttatc atgacatctt ttgtttcatt cacctcaaaa tggggtatgt tacttatata
    16621 atgataaatt ttaggagggt taaaaatgag taaagttttt gaagtaaaaa aagatatcta
    16681 ctttactggc gtagtagatg aaggtttaaa agtattcgac ataataatgg aaactgagtt
    16741 tgggacaact tacaactctt atttaatcaa ggacgaaaaa acggttttat ttgacactgt
    16801 aaaggcaaat tttaaagatg agtttttaag taatttatca gaggttactg atatagctaa
    16861 aattgactat gttgtaattc atcacacaga accagaccat gcaggtagtt taaaatactt
    16921 attagatata aatcctaata tagaagttta ttgtactaaa gctgctaaac tatacttaga
    16981 tggtcaaata aatagacctt ttaactgtca tgtaataaaa gatggtgaaa ttttaaatat
    17041 cggtaaaaga aatcttagat ttataactgc tccattctta cattgggttg atacaatgtt
    17101 tacttacatt gaggaggaca agactctttt aacttgtgat gcatttggtt gtcactttgc
    17161 aagtgttgat gcagaggtag tgaatagtga agattatcta aaatcagcta aacactacta
    17221 tgattgtata gttaagccat ttgctaaaca tgttttaagt gctgtagata aggtggtagg
    17281 actaaacata gaatttgata ctatattgac ttctcatggt ccaatgctta ctaaagaccc
    17341 aatggctgct gttaaaagat atgttgagtg gtctacagaa gctgttaata caactaatca
    17401 aaatcaagtt tctatattct acttatcagc ttatagtaat actttagaga tggctaaaaa
    17461 gataaaagaa ggacttgata aagaaggtgc aaaggctgaa ttatatgatt tagaagatat
    17521 gacattaaca gaaatgcacg atacattagt agtgtcaaaa gtaatattac taggctctcc
    17581 aactataaac aaaactatgg ttaaacctat gtgggattta ttctctgtta tagacccaat
    17641 ggctaaccaa ggcaagatag caggagtatt tggttcattt ggttggagtg gtgaaggtat
    17701 aactatggct gagactttac ttaaatctat gtcattcaaa atgcctgtag aatctcttaa
    17761 gaaaaaattc ttccctagtg aagagacatt aaaagagtgt atggcatttg gtgcagaatt
    17821 tgcaaaatta gttaaatagc cattaatcac acataatatt atttaaaata aatgatttta
    17881 tatttaaaaa taaattaaaa tggagttgtc tcaaaattaa tttttctaat ctaattttaa
    17941 gttcaactcc ttttttattt tctaaaatga ctgatataat gataaatgca ccctttgtgc
    18001 gagggtgcat tatttataat atgttctatt tgtacactag tatatttgta ttaattcaca
    18061 tatctagcgt atgctaccat ccctcttcca gaattagatg ccgtttcacc aatttccact
    18121 ttaataatta cattattttc tttacacttt tttgataatt ctgctgcaga aaggttctta
    18181 ccagtaaaat ctaaagaaaa tggcttatca tattcaaaag gatactcatc aggtacacca
    18241 tctggagcta atgcaaccat accatatggt gtattcataa accctattac agtaactgta
    18301 tgctttgatg ggtctaaatt tgaatttttg cttgtaaact taattgtatt tattgatgag
    18361 ttgattaaac tacacggaac tccctcatac atttcaattt ttggacgtag attatttcca
    18421 taaacttcat caagtattgc cccttgttga tctgctatca tagtgtgatt cccaggtcca
    18481 actggcgcag aaggcttagc tggcgtaggt ttcttaacag ttggcgttgg attctgtgtc
    18541 tttactactt cgccaacagc tggatatgct ttcttgtcaa atggtaatgg gtgagtaaga
    18601 ctcttgtata tgatgtctgc tcctgctgat ttgttggcat caatatctac atgatacaaa
    18661 cgtccatcct caaaacgaat aagattccac tgatggtcat ttgttgtact atgtacataa
    18721 taacattcaa ttcccatagc atcacataaa gctttcaagc tcctacacat tccagaacaa
    18781 gcaccttttt tagtaaccaa tgtataagca gtattggctc cctctgctgc gttccagtta
    18841 acatatgttg tgttgttttt aatgtgatta taagcaaccc taatcttagc atctgtggtc
    18901 atgttctttg taattttctt gttcataaag tctgctgttg cttgtgctac cgaattaagt
    18961 tgagcttgtg ataactttgg accatatata ctgttggttc cagctacata acttttcggt
    19021 gtctttgcag taacatcttt agctgtcata ccataaactg ttacactcat acatgaggtt
    19081 aaaaccatcg ttgcaatcaa tagtttagta gctaatttcc tgattttttt catattagca
    19141 ttctccttta taatatattt tactttcaat tccctaccat taattaagtt cattttagat
    19201 ttaaaatatg gattatcaac atgtatgcat agcaattcac gtggtcatag aatatttaga
    19261 tttcttggaa aatctgttct atatttccta tatagttcgt atacaatcca actcacaaac
    19321 ctgcattttt gatatcatca caataaaatg ataataatta atatattcaa aaatgttggt
    19381 ctgtaacagg tatgtcaatt ttccaattat tggtaatggc gagagtgagg agaattgtca
    19441 ataatacatt taaaattaaa attgtataca cttcatacat tctcccctaa caacattata
    19501 cagattataa tttgtatatt caatacattt tttggaaagt ataattatat caaaatatgt
    19561 gattatgtat agatacaatc cactatggag aaatataaaa gaaaagagta taacaaacaa
    19621 taaaaaataa gatgattcaa attttatttt gaaacatctt atttactcta caaattaaga
    19681 ttatttggtg aattatttaa ttattatttt ttattaaaga aattttaaat cttaatataa
    19741 aatacgctat tatacataat aatccaaatg caaatgctac tggaattaac atctccgatt
    19801 caaaataaaa gaaatattct aagaaaatat ctggatttac tatattccca ttcttgtcta
    19861 aaaaatcttt tccattatat cctaaatgct gtataaaatt acttatgtta ttataatccg
    19921 cttccctctc atataccata ttagcattca atccagaaga acttaaatta gtgtttccat
    19981 ctttcatatc tattacatat agtctaagag ctttgacttt actatactta tctttatcaa
    20041 gtattttttc aagttctaac ttatattcac caatctttgt catatcatgt ctttctgcca
    20101 attgctttat ttctccatca agttcttttc ctaaattata catattatgt tgtaaactat
    20161 tattgtaata ccacataaat ccagaaatta aaaatgcaat tatacttatg taacctaata
    20221 cttttttaaa ccccattaca attgatttat gcctatccaa tgataaagat agttctttta
    20281 ttatattaca taattcatac tgcatttcat caccatcatc aaatctttca attgcttttt
    20341 tacatgcttc ttcttcatct aatccttcta atttaaattc atttgcagat tctataagat
    20401 ggcatctcat ttcctgtttt aattctatag tacttttatt gtcacatttt ttatatagtt
    20461 tatctaaata ttcatctatt agtttcataa attaatccac ctctcccaaa aattgattca
    20521 taaccttttt aataaatctc cattcttgtt tctttatttc taaaaaatct ttaccttcat
    20581 tagttaaatt ataatatttt cttcttccac ctgaactttg ttcattgcta taataagatt
    20641 taattaaatt tttagattcc attcttttaa gtgcaagata cattgttcct tcttttaatt
    20701 caaactcact tctttctctt acttgctttg caatttcata tccataacaa tctttctttt
    20761 ctaatataga aacaattaat atatcgatat aaccttttaa cacttcttta tttaactgca
    20821 ttatgtacca ccgccttttc acctactact ctataatgta tagtacttat atttactata
    20881 ataatacata gtactctata atacaaggtt tatattctac aatagactta aaaataataa
    20941 atagctgtat attaatattt aatctataaa tactaatata cagctattta tcaaatttta
    21001 aaaataaatt tttgttttat taatgttgtc ataacaacat ttacttggtt gaaatagtat
    21061 tacctataag tttaaatata tataataata gacttgattc tatttcatat tattaaaata
    21121 gagtaatagt ttaaattcta ctactctatc tataatctat ttcctaatta tttaatctca
    21181 aatttagctc ttcatcattt aaatctttca taagatttct acaggtacta gatttattcc
    21241 aacagaaaaa ctagggcatt aaacacttat atatctgttt ctttagactg taatattaat
    21301 attttttaaa tacgttgact tttgcttttt ctaacttctc tccatgatag ttctcctcta
    21361 tcaagagctt ttataaatat ttctgctgaa gctagatttg tagctattgg aacatgataa
    21421 acatcacaaa gtcttattaa cgcttgtata tctgtttcat gagcctgaga tgtcaatggg
    21481 tctcttaaaa aaataactaa atctatttct tgtgttacta ttaaagaacc tatttgttgg
    21541 tctccaccta aaggaccaga tgctaatcta tttatattta attcagtttc atccattatt
    21601 ctttttcctg tagttccagt tgcatatagt ccatactttt ttaaaattga ttcataacct
    21661 atacaaaatc cgaccatagt gtttttcatt tggtcatgtg ctactaatgc tatattcata
    21721 tatctaatct cctcaattta tgtttagatg tatttaaatt tagaaattta ttttctttct
    21781 tctcatacac acatttagta ctaatcctaa gtttgctaaa gatgtcaata acgaacttcc
    21841 tccataactt acaaaaggta atgttactcc agttactggt attaacgcaa ccgtcatacc
    21901 tatattttgg attatctggt aaccaaacat tcccataaca ccaaccacta tgagggttcc
    21961 ataaaaatct tttgcatctc ttgcaattgc taaaagcctt aataggaata tcataaataa
    22021 gattatcaac actgccattc ctattacacc aagttcttca cctacaacag caaatataaa
    22081 atcactatct tgtacaggta aaaaatcttc ttgattttga cttccattgt aaagtccttt
    22141 accagttacg cctccagaac caattgcaat cagagattgc ataacttgat agtttccttt
    22201 tagagttaca tcttctggat ttagaaaagc ttctattctt accttttgat ggtctgccat
    22261 cattaaatac atcagtggca ttgacactaa tagtatgatt attcctcttt ttatcaactt
    22321 tgagctaaga cctgctgtga acagcattgc aaatatcata caacaaaata caattcctgt
    22381 tcccaagtct ggttgtgcaa tcaataatcc gataaatgga agagaataaa ctacaacagg
    22441 cataacttcc tttaacgtat ttaatttatc cttcttggat tcaagtatct ttgcataact
    22501 tagtacaaat gttaatttga ctatttcaga tgtctgcaag tcaagtggtc ctaagttaat
    22561 ccacgaccta gctccacctt ttactgtccc aattccaggt aataaaacta tggctaacaa
    22621 aataagactt attatataaa gcgctttata atacctgcct agaaggttat agtcaaaaaa
    22681 taatatgact ataatcattc ctataccaag tacaaaagct aatccttgtt taatcaattg
    22741 attatatgaa cctgttgaat tagcatgtgt tgcactacta agtataacaa gcccaaaaat
    22801 aaagatagct aatactgtaa ctataagttt ccagtctaac tgttttatca acttaattgt
    22861 cgacttataa ttaatattga tttttttcaa agaatcaccg tctttctatc tttaaacata
    22921 taattaaata aaaggggcat ataccaataa ggatatcccc cttttaagca ctaattacat
    22981 acatctatct tttatattct ttagtggtat attagcaact aatgcagata caggtttgtc
    23041 atcatgctca cctctgcatt tagacatttt tatttcaagg ccatcatctt ctatttctgc
    23101 ataattagct attactttta aaatgtctgt ttttatcatc tctaaaagtt gtggtgaaca
    23161 atctactcta tcatgaacta aaactagctt taacctctct ttagcaacag atttactagt
    23221 tttagcctcg ttagaaaaaa ctctaaataa atctaacaca gatgtatcct cctttgctac
    23281 ttagccatgc taaacatttt tttaagtcta ccaaagaatc catcttcaac ttcaagatca
    23341 agtagaggaa cttcttcatt tagtatcctt tttgcgatat ttttgtatgc ttgaccagca
    23401 agtgacttag aatcaagtat agctggctct cctttatttg ttgatataat tatgctttca
    23461 tcatcaggaa caagacctaa caaatctatt gctaatattt ctattatatc ttgtttatcc
    23521 atcatgtctc cacgcttaac catatcattt ctaattctat ttataactaa tcttatctct
    23581 tttatttcat ttgcttctaa tagacctatt attctatcag catctcttac tgctgatatt
    23641 tctggattag taactacaat agctctatct gctccagcta ctgcattttt aaagccttgc
    23701 tcaatacctg ctggacagtc tattattata tactcgaaag attcttttag tttctcacac
    23761 aggtcaatca tctgctcaac tgagacagca tttttatctc tagtttgtgc tgctggtaat
    23821 aaatatagat tatcaaatct tttgtctttt attagagctt gcttaagcct acaagttcct
    23881 tcaactacat ctactatgtc ataaactatt cgattttcaa gacccattac tacatctaag
    23941 tttctaagtc caatatctgc atccacaact actgtttttt tattttctag gcttagcgca
    24001 gttcctaagt ttgctgcggt agtagttttt ccaaccccac ctttaccaga tgttataact
    24061 ataacttcgc tcataatact accccctatt tttctttcta tttgtctaac tttgacaaat
    24121 agctttcgat tacaattctt ccattactta caaaggctat ttctgggcta atttctgatt
    24181 cactttcata attttcttca tcatctggag cttctgcaat attatctgct atttgtaata
    24241 ttttaggatt aagatttcca gcaacaacat atgcaaattc attgccttct gcacctgcat
    24301 gtacaaaacc ttctatttta cccattacaa aaacattacc tcctgctaca accttagcac
    24361 cagcatttat atctcccata acaactgtat ttgattttga aaggactttt gaaccaggtt
    24421 tcatatcatt caatataact acatcacctt caaattcaat attttcacca gatcttaggg
    24481 tattgacata ttttgttgga aaattactat tttcttcaat tttttgatct tcaacaaatt
    24541 ccacatcaaa tctagaagta attccttctt taagttctag aatctccatg tcagttaaac
    24601 aatcactatt aattttacta attttagccc cattaaaaaa gccaacatat gcttccaatt
    24661 tgtttattat tttttcctgt atttcctcaa atggagcttc tctttttata tttactatta
    24721 tccctctttt attacccttg aactctacta gttcttgact acaaatttct cttaaagaca
    24781 taacaacctc cagagtgata tatcatttat attcttcaaa tacgctttaa acccttttat
    24841 tttttctata taaagctaaa gtttatacat agattgagct atctattaat ttgtgactcg
    24901 aaatttatat tttcgtcaga agaattgtta acatcattcg tcttgttgtc atttttacta
    24961 ttagttggct ctaatcccat ataagttcca ataacttctc tagttggtaa aagtgagaat
    25021 acacttgaat caccttgagg aattacacat acaacagcta tttctgggtc atctgctgga
    25081 gcaaaagcaa cagtccacgt aaaagaacca taatcttgtt taaatctatc tatttggtca
    25141 tctgttattt ttggatttaa ttcttttata gcttttctta gatatgaaga attcacctta
    25201 tcagtgtcct ctaatctaac ttttactcca tcttctaatt cttcctctaa tttctttctt
    25261 tcttcgtctt tcaaatcttt attttctaat ttctttttta tttccttttc cttagctaat
    25321 gaaagctctt tttctttttc tgctttcatc ttatcagcta gtttaatcgc atcatttagg
    25381 ttgacattat aagatgacat atgacttttt aaatattcat actcgttatc tgtaggtatt
    25441 ttcccacttt tctcagctgt accagttttt gctgctacat ctattggaaa atcagcaaaa
    25501 gcactcttcg ctgtaccttg tcttgcaacc aatttcatac cttttgttaa ttcttttaag
    25561 ttatctggat ttttaaaagg tattttttcg actttcttct gatcattaat tttaactgat
    25621 gaataatcac ttgaaacagc tctatcaact acagataatt ctactaaatt acctccattt
    25681 gctatagcag ccacatatct tgatatctga gctggtgtat aggcattctc accttgtcct
    25741 atagctaagt tgaatgtatc tgcagtactc cattttgcaa agttaaaata actgaatact
    25801 gctaaatctg ctacttcttc aattctatct tctttaacat ccaatttttt tagcctattc
    25861 attgtttcaa ctctaccagg agttttcttt tctgctgccc agcttacaat ctcattgatt
    25921 cttttctcat attctttagg atttttttct ctagttatat caacaaaatc atttgccatt
    25981 tctctttcta aatttgactt tagaagagct tgtgttgatt tcaatttatc ttctgtgcta
    26041 ggaactttac ctgctctttc ttctacttca tcttgaagcc ctgtattttg gtcaagccca
    26101 aataattttg catactcaag tattttattt gcattcatat ctataccaat gctttttcca
    26161 tctggccatg ttttacctgt accaattgtt gccatatata tattacaaga ctcttgtatt
    26221 gctttatata agtttgtcat accatggttt cctctaccat ggttccatac atagtcacca
    26281 aaagattttt tacctaacat tataacccca gggtcattta tagtaaagtt tggatccaga
    26341 ccattttcta acgctgccat tgaagttacc atcttaaatg aagaacctgg ctgaaattcc
    26401 ccttgtgtaa ccaagtttaa aagtggactt cctgctagta agtcatttgg attttttggt
    26461 tgaagtgctt tataatcctc tgtagaaata ccttttgcaa atttatttgg gtcataatta
    26521 ggataacttg atgaagctaa tacttctcca tttttaacat caatagctat aagagctgct
    26581 gactgtgctt ttcctgcata tgcactaatt ggtttgtccc caaatttact tttaaaagta
    26641 cctcctttag aagcaacttc gattatctgt tttaatgcat tatctgaaac ttcttgtaaa
    26701 tctttatcta gtgtaagata cacagtatct cctgatttag gttttttaga ttcaatttct
    26761 ttactgattc tacccaatgc atcaacttta accattttat aaccatcagt tcctcttaat
    26821 ctactttcat tactcttttc tactcctgca agacctacca tatctccagt ttcataacct
    26881 ttttgaaggt atgattctat ttgagtggat ggcatttttc ctacatatcc taacatatga
    26941 gaagctaatg acccattagg ataatatctt actggttcta ctgctattga aacaccaaca
    27001 aggtccatag cactttcttc aatttgagca atcgtagttt ctcccacatc cttagcaata
    27061 gttactggat tatattgaga atatcctttt gattttatca aatcccttac gaccattatt
    27121 tttcttgcat cttcatcact caaactttta tcaatttcta atgcatcact atttcttact
    27181 ttttcaaacg cttctttggc actcagttta gtttctttta tcttataact agccaaccaa
    27241 tctctcttat ctctttctgc tgtgaacatc catttactaa ctaaaatagg tggataatat
    27301 ccattttcat ttagttttgc ctgtaatctt gtcgcatcca atcttttatc ttcttgagat
    27361 aatattccag ctgatataag tttgtctacc aagtaataaa aactttcctt agcattataa
    27421 tcatttggaa taccattttc tgatttatat tccctaatat ctctgtcata tgtataatag
    27481 tactttccat tttctacata tataggaaac tcatctacat attcttcacc atttctctcc
    27541 agcaaattta taagttttaa tgatatttca tttgctcttg aatgcttatt tgcatctttc
    27601 ttattaatat cgttacctga aacttgaaca gtaaataagt ttttatttcc tgccaacagt
    27661 cttccatatc tatcttttat ttctcctctt ggagcttcta ttgccaactt tttatatgtt
    27721 ttattctcag ctaactcatt gtaatattca tatttaaatg tagtcatata gattattttt
    27781 actaaaatag ctaaaaatat cacgcccatg actatcttaa ttatttttag tctatcaact
    27841 tgtttatttt cttccatgaa atcaccaact aatcttcttt aagttttaaa actgctcttt
    27901 tagataagcg atatattact aaaccaatta aactattcac tattggtgct actagtagac
    27961 cttttacaaa aagtgttgat atgttgtagc tttgataaat tatactagat gtacctatat
    28021 tgacaattga atcaattaaa ctgcctataa aaattaaaac gaaagtaata atataactac
    28081 ctttatatag ctgatctctc atgtatccaa ttgcatatgc aataataaac aaaattaatg
    28141 catttacacc aaaaattcca ccaacggtta tgtctttaat aagccctatt attgctgcta
    28201 taatcccacc ttctaattca tctaaataaa gagagattat ggttacatat ataagaacta
    28261 aattaaagct tatgccaaaa atatctatat aatttgttat actattttct atagtcataa
    28321 gcacaatccc caacagacaa agtaaaactt ttttcattgt ttttatcccc tttatcctat
    28381 atttcttggt tctatgacta caacgtcatt tatattctta aaatcaacat aaggttttac
    28441 aactacatat tttaatgact tatctttatc atcaaccact ttttcaactt cgccaattgg
    28501 tataccatct gggaagaatc caagacctga tgttgttatt atatctcctt gaattacatt
    28561 atatgatgag tcaaacatat atccttgcaa atatccttta ctattataac tatctttatt
    28621 atctaaagta gtattttgag taattgtacc tttagcattg gcatcctttg ctagtttaaa
    28681 gcttacagat gccttagaat ccaatagtga aattgcctta ctataattgt tggaaacttc
    28741 ataaactatg ccaactaatc cactaccatt cataactata ctttcttttt ttacacctga
    28801 attttttcct gcacctatga caaaactctc ataccagttt ccatcatttt taccaacaac
    28861 acttgctgaa atacttgtcg ccttatactc ttcttgtaca aagttcagtg tcttttttaa
    28921 ctcttctagc gattctgttt tatctagttt ggcatttaaa gctatcatat tttttttaag
    28981 tttttcattt tcttcttcta atttttttac ctttttagaa ttatctttaa aatttgctac
    29041 atctttaaaa ccatttttga taaaagaaaa tccatcattt attcccttac ctacacttgt
    29101 aatagcatct gaagctactc ctaaatttac tggactacca ctagaaaatt taccaattga
    29161 tatacccaca attccaatta aagtgatagc aactacgccc gttgctatca ctttaacatt
    29221 aatccttttt ttctcatttt tgttcttgtc aaatctcaag gccatcacca acttctaatt
    29281 cttagtattc atcattaata ctttttcaaa gatttcttgg tcttcaacag atttacctgt
    29341 tccaagtgca acacaatcaa gaggtgtttc tgctatttgt acagacatac cagtttcctg
    29401 tgtaataagc ttatcaagac ctctaagcaa agcgcctcct cctgttagca ttattccgtt
    29461 ttccattata tctgaggcta actctggtgg agttctttct agtgtagatt ttattgcatc
    29521 tactattgaa cttacaggtt cttttaatgc ttctctaact tctgtagagc atatttctat
    29581 tgttttaggt aatcctgaga ttaaatctct acctcttatt tgcatattta tttcttcatc
    29641 atctttaaat gtagaaccta tgttaatttt tacattttca gcagttcttt cacctatcat
    29701 aagattatat tcttttttta cataagccac tatagactcg tcaaattcgt ctccacctat
    29761 tcttatagat ttagcagtaa cgattcctcc taaagatatt acagctattt ctgaagtacc
    29821 tccaccaata tcaactacca tattaccttc tggttcttca acttttaatc cagcccctat
    29881 agccgctgcc attggctctt ctataaggaa tgcatcttta gctcctgctt gtcttgcagc
    29941 ctcttctata gctctctttt ctacttctgt aactccaaaa ggaacacata tagctattct
    30001 tggacttaca acaccttttt tatcagccgc tttttgtata aaatagctaa tcattgattg
    30061 agttatgtca aaatcagcta ttactccatc tttcataggt cttatagcta ctatatttcc
    30121 tggtgttcta cctatcattt ttttggcttc ttcacctact gctaatacct ctttactatc
    30181 atctcttatt gcgacaactg atggttctct aacaacaata ccttgtcctt ttatgtaaac
    30241 taatgtattt gcagtcccta aatctattcc catatccttt gtcatcttat taaaactaaa
    30301 aaaacctttt ttttctttct ttttttcctt agccatgatt ttactccttc ctactattaa
    30361 gaatcatgtt taaaacattc tcctattatt ttatcacata taaagtcaaa ttatcatatt
    30421 ttctttaaag ctaaagtata atccatctcc aataattata tggtctatta tttctattcc
    30481 aattatttct ccacatttaa taagtcttga tgtgatattt ttatcttctc ttgatggttc
    30541 tacacttcct gaagggtgat tatgcatcac tattatctta cttgcacttc ttcttatagc
    30601 ttctttaaaa acctctcttg gatggactaa tgacgagctt aaagtcccta tagatacatc
    30661 tacatcagat attatctcat tttttgtgtt taaaagaacc gctttaaaaa cctctttttt
    30721 taaatacctt aggctatcca tataatacct ctgtatatcc caaggattcc ttattttata
    30781 ctttataggt ttaaaactag ctactctaga gcctagttct aaagctgctt taatttgggt
    30841 tgattttgat aacccaattc catctatatt acacagctcc tctattgtca tatcttctaa
    30901 atgtctaata ccctgaatat ctctatttat tatatagttg gctaattcaa tagcattttt
    30961 attcttgttg ccagttctta aaagtatagc caacaactca gcatttgata agctttttac
    31021 gcctttggca agcatctttt ctctgggtct ttcctctggt gccatttctt ttaccttaat
    31081 ggttgaatta aatgactttt tcagataaat cacactccat aaaaaagatt tatactaaaa
    31141 tattttttta ataggtcgcc taaccttgaa attggaagac caactatatt aaaataatct
    31201 cctctaattt cttcaactaa caaagctcca taaccttgta taccataggc tcctgctttg
    31261 tcaaatgact cattagtttt cagataatcc tcaatatcct gctctgataa cgttttaaat
    31321 ttcacattgc tgattacata gtcaattacc tttttatctt cgcacaagtt tattaagcta
    31381 atacctgtta tgacttgatg ctctcttcca gatagtcttt ttaacatatc tcttgcacaa
    31441 gactcatctt ttggttttcc taaaatagca ttgtctaaaa caacaactgt atcagctcca
    31501 ataacaacat cttcattatg ttcagatgct acagacatac ttttttcaaa agctaatctc
    31561 ataactaaat gttttggtgt ttcaccttcc aatataatct catcaatttc atttttaatt
    31621 atatcaaatc ttacattcgt attctctaga atttctttcc ttcttggaga ggctgatgcc
    31681 aagattatat tcatttattc acctcatttg tacgtaattt gtacctattt agtctatcac
    31741 tatattgtaa aaaattcaac agaaatgtcg aacctttttt gttacactat tgttatattt
    31801 attaccatta gaataaatat acataaaata gaggtatgga aattattcca tacctctgtt
    31861 ttatgtactc attagagata tggaaattat tccatatcct tattttgtcc atattacaaa
    31921 attttaatag ctttctttgg acatttttcc atacataaat ggcatcctac acatttatct
    31981 gcatctacct tatgtttttc ttttaattct ccctctattg catcaaactt acattgtttt
    32041 ttgcacactg tacatccaac acaaagttct tggtcaatag taactttctt tttcttagtt
    32101 atgtcacctg atattacttt tgtagggcat tttgctacac attgcataca ccctacacac
    32161 ttattagggt ctatcttagc tattttgtct tcaaatatta ttgcatcaaa tttacaagct
    32221 tttacacaca ttccacagcc aatacatcca gcagtacatt tttccttaac agctttacca
    32281 aactctttgc tattacactc tacaactact tcttgacttt ctggttttgt gattattata
    32341 cccttaggac aaacttcttt acattttcca caatttacac acttttcttc atctattact
    32401 gctattccat caactataga aatagcatca aatttacaag catctttaca tgttccaaga
    32461 cctaaacatc caaatttaca catcttagca cctgagttta aaacatttgc tgctctacaa
    32521 tctgatattc cttcatactc atatttatct tttgcacttg aacaagttcc tttacaaata
    32581 acttttgcta cttgtttctc acctgcttca gcacttattc ccattatttc tccaactttt
    32641 gcaccaacat ctgctccacc tacagggcaa ccattaactg gtgcattgcc ttcaactatc
    32701 gcatttgcaa gacctccaca acctggaaat ccacaacctc cacagttggc tcctggaagt
    32761 actcctagta tagcttccac tctctcgtct acttctacag caaacttttt agatgcaaaa
    32821 tctagtacta tcccgaagat taagcccata attcctaaaa ctaatacagc tgtaagtatc
    32881 accattttca cccctctttt ttatagcttt ataagtcctg taaatcctaa gaaagctatt
    32941 gacattaaac ctgctgatat aagcgttata gggaaacctt taaatgcttc tggtatatca
    33001 gctaattcta gtctttctct tatacctgcg aatattacta ttgctaaagt aaatccagct
    33061 ccggctccaa atccgtttat tattgtttct actaaattat aacttttttg aacattaact
    33121 agagctatac caagaactgc acagtttgta gttataagag gtaaataaac tcctaatgct
    33181 tgatataaag atggactcat cttttgaatt accatttcaa cgaactgaac tatagaagct
    33241 ataaccaata taaaagctat tgtttgtaag aatccagttc cagtttttac taataaaatc
    33301 tgtataaaat aagttataat tgaagctaga gttaaaacga acgtaactgc aactcccatc
    33361 cctactgctg tatcaacctt cttagaaact cccaagaaag gacatatacc caagaattga
    33421 gatgttataa cgttattaac aagaacgata cttaaaaata aaagtattaa attcatttat
    33481 tacacctcca ctatgctttt ttacttctta atttattaaa tcctgcaaat aagaatccta
    33541 atgttaagaa tgcacctggt ggtaatataa atattaatac tggttggaaa gatgctccaa
    33601 ataatgtcat tccaaataaa ctaccatttc caagtaattc tcttactgca cctattattg
    33661 taagtgcaac agtaaatcca agaccttgac ctacaccatc aactattgaa ggcatagctc
    33721 ctgttttgaa agcaaaactt tctgcacgag ctaatattaa acagttaaca actattaatg
    33781 gtatatatat accaagagcc ttatcaagag caggaacata tgcttttaaa agcataccta
    33841 ctattgtaac aaatgtagct ataactacta taaatatagg tattcttatc ttatctggtg
    33901 taatttttct tattaaagat ataactaaat ttgcacatat taaaactgct gctgttgaaa
    33961 gacccatacc aattccattt attgctgaag ttgtaacggc tagtgttgga cacataccta
    34021 taacttgaac aaatgttggg ttctcatcta ttagcccatt tttaaatact ttagctaaat
    34081 tcataattaa ccctccttat ttattaagag cactattgaa aacatctata gcagcattta
    34141 ctcctgtagt aactgctgta gatgtaatag ttgcacctga tattgctaat atttgattat
    34201 ctgctgttgt acttcctttt gctacttcaa gaggctcagc agtttttcct ttgtattgac
    34261 ccttaaatgc atcatcctta gcttttgctc caagacctgc tgtttctgac atattaccaa
    34321 tgtcaacacc tgtaacttga ccttcagatg atatacctac cataacttca atttctccac
    34381 catatccact tggtttagct tttaaagtat atccaaccac ttcagaacca tctaaacctt
    34441 catacacttc agctataaga tcatctccaa ggcctttgta agtatcttcc ttcatttctt
    34501 taaattcttt tgcttctgga aggactgctt ttctaagttc attatttgct tgtatatttc
    34561 tttgttctat tactggagct gtaacctgat tagtagctcc aagagctaat gcagctattg
    34621 cacttatagc aagtaaagta ccacctaatc tcaccatact gttcattatt tagccacctc
    34681 cccaaatact ctatttctaa caaacttatc tataagtgga gcaagacagt tcactaatag
    34741 tattgagtaa gatacacctt ctggatatcc accatacatt cttataacag ttgttataag
    34801 accagctaat acagcgtata ttatttgacc ttttttagtc ataggcgaag ttgtataatc
    34861 cgtaagcatg aagaatccac ctaacataag tcctccagca aacagttgat atattgcgaa
    34921 agttatatta aatccaccta aaaggaatgt taatataaat acagttgcta tgtaaaatac
    34981 tggtataaca taatttatta tgcctttata cattaggtat actccaccta ataataataa
    35041 tattgctgat gtctcaccta tacatccacc aatattccct atcatcatgt cagcaagact
    35101 aattccattg ctagaaaggt ctgccagtcc tgctgctcca tttttcaaaa agcttaatgg
    35161 agttgctgta gtaactgcat ctaaattttt acctatccag ttaacacctg gttgagtcca
    35221 agcagtcatt gctactggga atgatgctaa taaaaatgct cttgcagcaa gtgctggatt
    35281 gacaaagtta catccaattc caccaaatac cattttaact actaatattg caaatattcc
    35341 tcctactaga cacatccacc aaggaagtga tgctggtacg ttaaatgcta ataataatcc
    35401 tgttacaaca gctgaaaaat ctcctatagt acttttttgc ttcgtaaatt tttgatataa
    35461 aaattcagta cctactgtac caagtataca aaaaaacata gcactcaaag cattaagtct
    35521 aaagaagtat actcctgcta ctgctgctgg aaggagtgct ataataactt gtttcattat
    35581 atatgaagta tcttcattac ttctcacatg aggagaagat gatactatca acttattttc
    35641 catcgttttt cccccttaac tttctttatt tctgagcagc ttgctttctt ctctttgctc
    35701 ctatttctct ctttgctgat cttatagatt gaagtaatgg tcttcttgct ggacaaataa
    35761 atgagcaaga cccacattct atacaatcca ttgctctatg atattcagct gtgtcatagt
    35821 catcttttaa agaatatgca cttatgaaaa gtggttgtaa gaatgatgga catacatctg
    35881 tacatcttcc acatcttaaa cagttttgaa tatctggagt acgtgattct tcctcatcca
    35941 aacataaaat tcctgaagaa cctttgttag tcgctatttc agtagtatat tgagctattc
    36001 ccatcatagg tccacccatt ataaccttac caatcttctt atcttcttta aatccaccac
    36061 attgttctat tatttctgaa actaatgttc caacttttgt tattaggttc tttggctctt
    36121 tgatgcaact acctgttatt gtagtgattc tttcaaccaa aggcatacct gttttaatag
    36181 acttagctat ctgtgctgct gtggcaacgt tgtcaacaac tgcacctgct gctattggta
    36241 atccaccaga tggaacttct ttgcctgtac acgcataaat aagttgtttc tctgctcctt
    36301 gtggatattt cacttgaagg ccaacaactt cgatttcgct atagtcttta gctacatttt
    36361 gaattgcttc tatagcatct ggtttattcg tttcaattcc tataaatcct tttttgacat
    36421 ctaatacctt cattaatgct cttaaaccaa acacaacatc ttctggattt tcaaccatta
    36481 atctgtggtc tgcagttaag tatggttcac attctgcacc atttaatata actacttctg
    36541 ccttgctatc tggtggagga gatactttta catggttagg gaatgtagct ccacccatac
    36601 ctactattcc tgcctctttt atgataccta ctatatcttc tttgctcaaa tcctctaact
    36661 gaccttttgg ttgaacactc tcatgtaatt cttccttaaa gtcattttcg ataactacac
    36721 attgagctga acctcctggt acttcatgtt gttcaatagc aataacttta cctgatactg
    36781 aagaatgaac gttacatgaa acaaaacctt gttgttcacc tattttttgc ccaaacttta
    36841 cttcatcacc aacttcaaca ataggtttag ctggtgcccc tatatgttgc tgaagaggaa
    36901 tgtaaactat ctttggaact tgagcttttt caagtgcctt tgtgttagag tactcttttc
    36961 tatatggagg atgtatccct cccttaaaag ttaagagttt cattgtttta cccccttatc
    37021 tataaaaaaa cattttttct gccttctata agtatactat atatattaaa aaatcttaac
    37081 aattgttaaa aaaatatccg tatttttata atttatttta taaatcaaca cctctatatt
    37141 aagtcaaatt gaggaaaagg attgcttaat taaacaatcc ttttcatata gctttctaaa
    37201 gtccaaattt tctgtatttt attttataga atttataaac atagagtact cctgcatgga
    37261 tgataaaagc agacttttac ttacactata tttttcttca tttgctgctt ttgaagctgt
    37321 atcaattttt tcattcacat tctttacata ctctattaaa ttatctttaa ataaacgcat
    37381 ttcagaataa tcaatctttt cagcttcttt tgatatattt tgtgatattg cctttctatt
    37441 tgtcaaaata gtattatatt tttccatatc tacattattt tcagcattcg accaaaaact
    37501 tgattcttct ttaaagtttg ttattaattt atttaattct ttagatatac tctctatata
    37561 agcgtagtta cttgtatact ctaaagacaa cataggtgca tctaagtgtg ataaaaatgc
    37621 atctggaaac acctttctta cactgcttat ctgctttcta acatcatttt catcaaaaga
    37681 ggaataggtc tgtatttttt ttactccatc cttctccata actgaaaaag gtatgtcatt
    37741 attaactaat gatgtctgta tttttttcaa atcatcatta ttatctattg cagcaacttg
    37801 aatagtatac atatcccagc cttctatagt agctaatttg acttcttctg gctctttagt
    37861 gttatttacc gctttttctg gattagtttc ttgtttatca tctgaattgg tttgtgattt
    37921 agattttttc ccttttttta ttgaatttaa ttcatcagat atatctttat aagttatatc
    37981 tgatgaatta tttagaaatg gtattttagc cgatacatac tccaatatct tgctatcttt
    38041 tatttttgta taagaataat agccaattag acacaagcaa gcaatcgtta taacatattt
    38101 acgtaaatca atcttatgtc tattattatt aaatcctctg tatatcgttt ttcttctatt
    38161 cattagtttg tccctcccaa aattatatta ttaataaagt atatttttaa gagtcacaat
    38221 ttatgaatat aaataattat ttcttctaat tttttaataa gaaattgtac ttactaaaca
    38281 aatcttcttg caccaagtaa tactttagac caatatgaac tagttaaatt atccacttta
    38341 accttattag gattagtctg agtaccacta caatggataa atttactatt tcctacatat
    38401 atacctacat gattagttgt tccatcacca tcagtgtcaa aataaactaa atctcctggt
    38461 gcataattcc ccatagaaat tgcacttcca aaattagcct gttctctaga aactctagga
    38521 atagaaacgc ctacagaatg tttaaaaaca tactgtgtaa atccagaaca gtcaaattta
    38581 tcaggccctg aagctcccca ttgatatggt attccaataa gagtatatgc aaaatctacg
    38641 attgttgaac cttttgctgt agatttatct gaatttccag taaatgatgg ctttgagtta
    38701 ctattaggtt gagaattttg gttagatgat gaatttggtt tttcttttgt accttcatta
    38761 gtctcagaaa tataacttgc actcacccat ccaattgttc cactcgataa ctttacctta
    38821 taccaaccat tacttttagc ttttaattca acaacatctc caccatttaa ttttcctatt
    38881 atagaatagc ttgtcccagg accacttctt acatttaaac tcacagcaga tgttacctta
    38941 ccattcttgc cttcaattga ttcttcgcta gatggttttt tatcagaatt attagtacta
    39001 tttgaattac tagaattgtt cgttccaaca tcttctgatg ttttagaaat atattggcta
    39061 ctagcccatc cagtaactcc atttgatagt tttattttat accatccatt gctttgttct
    39121 agtaacttta ctacatctcc attatttgct ttaccaacta atgaatagtt agtacctgca
    39181 ccacttctta cattcagtct agaactcaca tttactttac catttccact aatagttgtc
    39241 ccacttgaag ttggattatt ttgacttgat gtaccttcag acgaaccact tgttgatatg
    39301 tattttgcac ttccccatcc aacaacagaa ctcgataccc ttactttata ccatccatta
    39361 gacttttcta aaatctctac agtttttccc ttgtataatt ttgctacctt atcgcaatca
    39421 gaacttggtc cacttcttat attcaaggca cttgctgtta ctgttccctt ttcaagagca
    39481 cttgcattta tggagctaac agatactgct actgccccaa ttcctaaagc tgctatagct
    39541 tttttcacta ctatcactcc ccccaacaaa tttttatagt cataaattca taacattatt
    39601 atccattttt agtccctaca atttaatgat aacttatttt cttattaatt gtaaagaaat
    39661 tataaacata tccttaaaat tagtgatttt taggaagtat ttcaatccaa taattatctg
    39721 ggtccgctat aaaataaatt cccatacttt cattttcaaa acatatgcaa tctaaatctt
    39781 tatgaagttt ttttgcctca tcaaactcct caacttctaa agccagatga aattcattgt
    39841 ctcctaaatt atatggtcta tcccaatctc taagccaagt taattctaat gtatgactag
    39901 taatgccatc tcctaaataa actaatataa aacttccatc ttctgcttcc ttgcgcttta
    39961 cctctttaag ccctaaagct tttttataaa aatctaaact tttttctaaa ttagtaacat
    40021 tgaaattgtt atgacaaaaa ttaaacttca ttttgaaatc actctccaat ttttattttt
    40081 tttatattat atcattatta taaatttatt taaatcatat taagttaaaa atttgaatga
    40141 tgatgaccaa ttaccaaatc atggtaaatt atttaaaaga atatgtcaaa aaaagaataa
    40201 gctctttcaa atgcataaaa aatttatacc caagttatac ttaatcatgc aaattggcag
    40261 aacttattct ttaaaactct aacttttaag tttttcttta ataatataat ttattacttt
    40321 ttttctaata tcttcagcat tataatcatc ttctacttct atatccaaaa gttttgctat
    40381 tgacctcata tcttctctag tctttgacct atagcttgat attaattcat cttttatttt
    40441 ctctggttct aaaagatatt cttcatctcc tcttttgaaa acaacgtatt tttgagcata
    40501 tgaacttcta ttgtagtata tatatcttga tatttttctt aaaatttgat tatatgaaag
    40561 ttgcttgtta atatcaagtt ttaagtactc tgaaaaagca aatagctctt tttttgattt
    40621 aaataatgct tctttcttta tagttcttga tattgaagtt acactttctt gatatgcaca
    40681 cttttttaac atttttaaat atactctatt aagaccaata ctttgattga atttttttat
    40741 attattatta ataatcttat ccaagttacc ttcaaagtat ttttttgctc ctttttgtga
    40801 tacataactc tcaagttctt tgtgggccat atcaagtatc atttttttta aatcctcttt
    40861 atatccccta gattccatga tactccctcc cttagctatt tatagtatat tcaaaaagct
    40921 caagaaatgt gagtcattac aaaatttttt tactttttat gtatatatta atttaaaagg
    40981 ggagataata gtaatgtaaa atcaaacata ctcaatctcc cccattttta gacctctatt
    41041 actcccccaa tagaggtctt tttttagttt atcaatttct attcaattcc ctttagttca
    41101 aatcctaatt ctgctatcac ttcttttaat ttttcaatag atacatcatc tttcatatct
    41161 acagatgcac acttattttc taaatctaca tctaaaactt caataccatc tatatcttct
    41221 gttaatgcag ttttaagatg gtttacacaa tgaccacaac tcataccttc tactaatagt
    41281 ttcttcttca tttaattacc tccttatacc ctataagggt attctatcat atagtttata
    41341 ctttttaaat agaaattaat catttaattt gattcattcg attttatatc taaattagat
    41401 attccctcat atacatcaaa atcatttgga gtaattttac cttttaatct actattactt
    41461 acaatatagt tgtttctcac agctaaactt ataaatgttg cattatcttt tatatatgtc
    41521 tgtacatctt tgtatgcttt ttttgtttca atttcagacg tagaacttgt aagtgcagac
    41581 atatatccat ttaacttatc atcagtatat ccactactcg ctataatact acttgcatct
    41641 ggtataattg atagtttcca tccaactaat gctaaatcat aatttttaga attaagagca
    41701 ttttccatat ccttatctga cagctgattt actgtactat ttattcctat agcttttaag
    41761 ttttcattta ttatgtttgc agtttttatt ctctcactat tatctctgtt tactattatc
    41821 tttaagttca tttcagaaag acttttcttt acttcctctt tttctctatt ttttttctct
    41881 tcagcttctt ttttgattct atcttcttct gcttttttag cttttgcttc ttcttctggc
    41941 gtaagcttct ttgagttctt tttagctgat tttttctgat tttttacttt attgtcattt
    42001 tgatttactg agtttacatt tgcatattct accttagcaa gacactctat cgccttatct
    42061 ttattaaact ctaaatcctt catttcacta ttatagtatt ttgattttga attaagtgga
    42121 aaattaactg gagtagcatc atccatataa ccttcactaa taattctatc tttatctata
    42181 gagtgtgcaa ttgcttttct aaaattaaca tctttgaaaa aaggattatt ataattaaat
    42241 aatacacact cataatctct tccttgatat tttgttatat taaactcttt ttcttgaaac
    42301 ttacttaaat catttaatgt aacattggtt atatcactat caagagccat aaccatagat
    42361 gtccttgcat cttcattagg caccatacct acttttatgt tcttcatggt ttttggaact
    42421 tcttcatagt aatctttgtt tacagataaa accattcctt ctctttctgt atatgattct
    42481 attttatatt tcccatttcc tatcatatta ttattcttgt catttacatc ttttgaatct
    42541 aaatgatttt gagatacaat tggaaaaatt aaactatcaa ttgaaaaagc atatctagct
    42601 ttaaatttta ttgtaaaatc tttatctgat ttaatattca cagaagctat atttttagta
    42661 aattcattat atggactatc tgcacttttt tgaattaaac caatagtata gtttacatca
    42721 tttgaagtaa cattttttcc atcatgccac tttgcatctt ttagctttat atctatactc
    42781 attccatcct gagctatatt atactcctta actaattgag gaattacatt gtagttctca
    42841 tctatagtaa ataaactatc ataaactaaa ttcattatat aaccaacaga cttatcagta
    42901 ttagttatag gatttatagt ttttggagtt accattgtta agtttatata cttagcatct
    42961 atagaacttt gactttgtaa acttgaatca ctactacttt gttttgtttt agtattacta
    43021 caaccagcta ccataagagt tatagctaaa acgactgtta agacttttat tctcttcatt
    43081 agttttttcc tttcttgcca tatatcttat tataatgttc gtaattattt tttacttttt
    43141 ctacatattt tgaagtttgc ttaaaaggta tatcatgtag attttcgcca tctttactat
    43201 attcattatt ttctaaccat tttttcacat tacctgaacc accattatat gcagctatca
    43261 ctaaatctaa tttaccaaat tctttgtata acttacttaa ataccaacag ccaagtctta
    43321 tattagtctc tgggtcgaaa atatccacat tttttaaatt tagttcctct gctccccaat
    43381 ctctagttat gtctaatatt tgcattaatc cttttgcttc cttttttgaa acagcagaac
    43441 tattaaactt actttcggct ttaataacac tgtaaactat attttcatct aaattaaatt
    43501 ctttcgaata cttttctaca tactctgaat attttttagg atataaaaat ttatgtatta
    43561 ctttgctttc cattaatagt gccccaaata agattataaa aatagataga atcaatactt
    43621 tcttactatt cacgccaatt ctccttcatg tactcaataa atttatgtgc tttactttct
    43681 aattcagtta ttgttcccga attatctatt atataatcac catatttgga tttttcttcc
    43741 tgactcatct gtgaatttat cctactaagt gcttcctgct cactgcaatt atctctcttt
    43801 tgtattctac taatttgtac ttcttgttta caagtaacta ctaatagctt gtcaaccata
    43861 tctaaaaaac cactttctac caatattgcc gcatctaaca ctactatatt ttcgcctttt
    43921 tttctcaatt tttctatttc acttattatt ttttctctta ttcgtggatg agtcaagtta
    43981 tttaattcct taagtttttc ttcatcacta aaaactattt tcccaagagc ttttctattt
    44041 agagttccat catcattttt tatactttgc ccaaaatgga caaatacttt ttccaataac
    44101 aacttatcct caaatatctt cctggatata atatcagcat ccactatagg aatgttaaga
    44161 tttctaaata tatttgatag gctgcttttt ccacagccta ttcctcctgt aagtcctaat
    44221 atcaacataa aatcacctac tttgtctcat accaagaact acctacattt aaatctacat
    44281 ctaagtgaac atccatattt acagcatttt ccatttcttc ttttactatt ttcttaacta
    44341 tatctatttc atcatcaatt gcttcaacta tcaactcgtc gtgaacttgt agtataagtt
    44401 ttgatttcaa attgttctct tccaattttt tatgaacatt tatcatagca atttttatta
    44461 tatctgctgc acttccttgt attggtgcat tcattgcaaa tctctttcct ctatttcttt
    44521 ccataaaatt gcttgacttt atttctggta tatatcttct tctgtttaaa attgttacag
    44581 catatccatc ctcactagcc ttttctactg ttgtgtccat aaactctttt attttatggt
    44641 atttagcaaa ataactttcg atatattctt tcgcctttgg aacaggtata tttaaatcat
    44701 cagctagacc aaagtcactt ttaccatata taataccaaa gtttacagct ttagctgcac
    44761 ctctcaattc actagtaaca tcttccatag gaacattaaa aacttgtgaa gctgttttgg
    44821 tatgaatatc ttcatgatgt ttaaatgctt ctatcatatt ttcatcctga ctcatatgtg
    44881 caagaactct tagctctact tgtgagtaat cggcatcaac caacttacag ttatcatctg
    44941 caataaacac ttttcttatt tttctaccca tttccatttt tataggtata ttttgaagat
    45001 ttggttctgt agatgaaatt cttcctgtag tcgttatagt ttgattaaat gatgaatgta
    45061 ttctattact ttctggattt attatattta aaagtccttc aacgtaagta gaatttaact
    45121 ttactatttg tctatactca gttatcttat ctattattgg atgcttatct cttaattttt
    45181 ctaaaacatc tgcattagtt gaatatccag ttttagtttt tttaattatt ggaagctcta
    45241 atttttcaaa taatataaca cccaattgtt ttggtgaatt tatattgaat ggctctcctg
    45301 ataattcata aatttccttt tctaatgtag ctattatttc tttaaactct acactcaatt
    45361 catccaatat acttttgtct acttttatac cttcatactc catatgacct aagacttcta
    45421 ctaaaggcat ctctacatga taaaaaagac catccatact catatccatt agacttcttt
    45481 ccatcatagg aactgtttct ttaactgtgc atattatttg acccatatac tctgctaaat
    45541 cctcaaattc taaatcctca tagtttttag atttaacacc ctttcctaat aattcttctt
    45601 ttgatttaac cttttttgat aaatacttca tagctattgc actacaatca tatgaggtag
    45661 atgaagttga atctattaag tactcagcta ttgttatatc aaaatacata ttttctaagt
    45721 ttatattata tggttttaaa gatatataat catcttttaa attatatcca tactttttaa
    45781 tttcattatt tgaaaatata ctatctaact ttgacacttc atttgaatct acataatata
    45841 ttttttctcc atctacactt ataaaaatat atattatatt tttttcaagt atattacctt
    45901 gtcctctaac agtttttaaa ataacttgtc cttttttgtt tacttcatct ataaattttt
    45961 ctatatcttt taattttaat atttctattt tttcttctac gtgttctgtg tcatctgaat
    46021 cgaccaaatc aactaatctt gataagagac ttgtaaatcc aaagtatcta aatttatcta
    46081 aaagttcatc tttatcataa tcgccaaaaa tcatactatc taaatccact tctattggca
    46141 catctcttat aattgttgca agtcttttac tttgaatagc tatttctttg ttttcctcta
    46201 ttttcttttt tacactacct ttaagcttat ctgtattctc tattagattc tctatactag
    46261 aaaactcttt tataagcttt atacctgttt tttcacctat tccaggtact ccaggtatat
    46321 tatcagactt gtctcccata agacccttta aatcgataaa ttgtgttgga gtcatctcat
    46381 atttttcaat tacagaatta aagttgtatt cttctacttc tccaacccct tttttcgtta
    46441 taagggtagt tgtattatct gaagcaagtt gaatagcatc tttatctcca gttactatgt
    46501 ataccttata tccattttct tctgcttttt tagaaacagt tcctataatg tcgtctgcct
    46561 catagccatc tatttccaat ctatttattt tgaatttatc taaaaggtct tttaatggct
    46621 gtaattgttc tgccagttca tctggcattc cctttcttcc agccttatac tctttatatt
    46681 ctaaatgtct aaatgtaggt gcttttctgt caaatgctac acttatatgt gttggtttat
    46741 atatatctat cattttaaat agcattgttg taaatccata tattgcattg gtctttagcc
    46801 cttctttatt attcatttct ggaagagcat aaaaagctct atttattatt gaatttccat
    46861 ctattataat taattttttt tccaaattat atcactccta aaaattatct atttttgtaa
    46921 catatatatt atataccaat atactgttat ttaatattcc cataataata atagtatatt
    46981 aaaattaaat atgttgcaaa caatatattt ttttgctata ataatataca tgacatgccc
    47041 gagtggtgga attggtagac gcagtggact caaaatccac cggactaata ctccgtgccg
    47101 gttcgagtcc ggcctcgggc accattaaaa actcctaaag ctatttattc aatagattag
    47161 gagttttttt atttttgatt taaattttaa gttatttttt gaaattactg tgtcttttta
    47221 aaatttatat aattatataa atttatcttc tagcattaat ttaaaaacta atttattttt
    47281 atataatttt ctattttata tcataactta ttacaacttt ttatctaatt tattgtatac
    47341 aacaacacta caaataaatt gaaataaaat aagcaatcca agaacaacta aaacccactt
    47401 gcttattacc attacagaaa atacactcaa tatagaaaca ataaatataa gaattacaag
    47461 aacataggac atgatttgat tagtgatacg tccagcttta tcacgtaaca taattcttct
    47521 ttcatcattc atctctatcc tttgattttt tatcttttct tcataatcta ctgctctttc
    47581 aggtttactc caatacatat attgaagaat catacataca cctggaccta aagcagctcc
    47641 agataatccc caaataaaag cctcaaatga aaactcagta aatgtagcca ataaaacaag
    47701 taaaattcca caaataaaat atataattcc aacatataac atattctttt tcatttttac
    47761 tcctcctcat aaataaatat ttcttcaata ctcatatcaa agtatctagc tatcttaaag
    47821 gctaagatga tagaaggatt atacctacca ttttctaatg aacctatagt ttgtcttgat
    47881 acttgcaaaa tagaagcaag ttcttcttgt tttatgcctc tttcttttct tatttcttct
    47941 aatctatttt tcacatctat ccccctttaa tatctcaact acggaaagtc tactttccat
    48001 aattatacaa ctatattcca tttatgtaaa gcttactttc catatttaca aaaaaataac
    48061 agactatttt cagcctgtaa ttttagaatt tcatgttgtt tattaatttt aatgttattt
    48121 attaattcca tattatttat taattttaac ttggtttctg aattggaatc caaacttctg
    48181 tagtataatc aggagcattg atatcgccat tgggataaag ttcaatatct ggtaaattag
    48241 catacacata cccagaactt ggtaaccact cagtaataat tcttttttgc aattcctgta
    48301 ttgccagagc atttggcatt ggagaaacac attcaaatac tgcccatgtg cctgatggta
    48361 ttaagtattc atgagtatct tctaatacag gcttatctgt tggagcagca atataataat
    48421 ctaaaccctt actatttgta cacacactaa ctccaagcaa tccacaaggt tcattattta
    48481 ttaactctga aagtttatca attgttccat tttttatgca tttagcccac aacttaggaa
    48541 ctttctcaaa gcactcttct acaatcatgt tcatactttc ttttatacca acaatacgaa
    48601 accctttctt ttctacaata cgatattgca tttctatatt tccttttaca taaatgctta
    48661 atgtcatctt aggatatgct tttaaaacaa caccctttgc tctagctgca gatggagaaa
    48721 tgttatgtat attttgaaaa gctctattaa aagatgttgg tgaatcgtaa ccatacttct
    48781 cagatagttc taaaattttg atgttactgt tctgaagttc aaatgcagcc ttagtcatac
    48841 gtcttcgtct aatatattca gataatggta cttctgcaat atatgaaaac attctttgaa
    48901 aatgaaaggt tgaacaacat gcaatttttg ctgcttctgc ataatccact ccactttcga
    48961 gattttcttc aatataagta atagcttgat ttaatttatt tatccattcc atttgatatt
    49021 acctccaagc aaattataca gtttaataaa ataatatacc tctctattca tgcacaaaat
    49081 tgtaagcatt taaattcata agtatttttc aatcaataca ataaatataa aaattgtatt
    49141 atacaataaa aaagttcaat aacattatat tataaaatgt tattgaactt tttataaaaa
    49201 ttaatttaag tgtagttaaa ttaagtatta cactatctta tatattacaa tatcctacta
    49261 tcttatagtc ttctggcact ttataatctt tgtctggagt acctaaattc cattttaaat
    49321 caaacatagt tgcacttatt attgttgcaa aataaagcat aactatacca atgtctattt
    49381 tttcttcata taaattagta tgaccatctt ttctaacagc taaaactaca gtgcctccat
    49441 caactataaa tctccatggt tgtctattta atgtagatgg agctaatctt gcatagtgaa
    49501 atgcatcaag aagtgctctt tcttctaaga tttctatact tgcaacattt ccccattcgt
    49561 tcatataaac aacatcttct acacctagtc tagtagaccc cttatctgtt acaacttgca
    49621 tatcagattt tgaataattt tgcccagttt ttgtagcatt taatacttta gcattactta
    49681 cattttctcc atatccaata gctataattc ctgttacctc tttatctgaa ttaattccaa
    49741 gtttttcttt tacctttttt ccatcttcaa aagtaaccca acaagaacca attccaagtt
    49801 ctgttacttt aaagattata ctttctccta tgtaccctga attttcaatg taattatctt
    49861 ttatgtcaga taatattata atataacttg gagaatctat catatttcct ttgtaccctg
    49921 ctactttatc taaattatta aaaacttcac ttctattcat aattcttaca tcaacatcta
    49981 tttctggaac tagtttctta caagattctg agtatttctt aatttcacta agatattctt
    50041 ctttaatatc tgtgttctta tattctctaa cagactttct atttaatatg attgttttat
    50101 aatccataat tttatcctcc ccaaactagt attgtcttaa ttatataccc attaatatat
    50161 tattttactc atttatttaa taaataaaga ttacaatttt gtaattatta tttattaata
    50221 atattccact tttacattaa taatgaccag ttgtgcaaaa tccattgcca accaccaaat
    50281 aaaaacatat tataaaggta atataaaata acaaacaaat cttaacaaaa aattaaactt
    50341 tttttacacg aaagaaggct tcccgttgcc ttctttattt ttataaaaaa ataagctgtt
    50401 tctaaaatga tttcacattt ttgaaacagc caagttattc tataatgaca actataattt
    50461 gaggatgtga gggagagttg tcattataaa tttagggtaa ttatattatg ttttaagcta
    50521 gttctttcta tatttatata atacatttta tatatgaatt ttatatgaga ttggtataaa
    50581 tgatatatga aattacatat gtttatatta tattaaattt tattattaca ttttccacaa
    50641 caatactcat atgtctccac attatttgga atatttacaa atacaaattc tttacagttc
    50701 tcacaatact ttaatataga tatagttcca caagtgtaac attcaaatat tacacaatcc
    50761 tcattttcct ctactatttc aaacttttta ctatgttcac cacactctgg gcacttgtga
    50821 ttatttacat tcataaatac tcctccttat tagtatagtt acatgtcaaa aacttaatta
    50881 atgtattcta tattacttat tatatattct ataataaatc atattctaat ataataaaac
    50941 aaattaaata aaaaaaaacc ataaatttta aatttatgga ttaattcttg taatgataac
    51001 tctatttgag gatgtgaggg agggttatca ttacaatagg gtattatatt atggttttta
    51061 agctagtctt tctacacctc atatgataca tatgctatat gaattttata tgatttttac
    51121 ataaatattt tatgaaattt aaatttttta gtcattttga aatttgtaaa aagttctcta
    51181 aaaatccaca gcatcataaa cagccaaaat ttaagtaaaa catcataaaa aaatttgtcc
    51241 aataatttta acaagtaatc tcacctcatt ataattattg acacaaactt aaataaacaa
    51301 tctataaatc ttctataaat cttaaacttt ccccctattg atacacaatt tctagtttgt
    51361 caattttatt ttgcatttct aagaaaattt cttcaacaga attgttaaat atattgccta
    51421 aatagattct tttatctttt tgaaatgaat aagattttac actaccatca gaagaaatat
    51481 ataaatgact ccactctgct ccatattttg aaaacctctc ctttgtatct ttaaacctca
    51541 tatcttctat ttttattgct ttttctctgt ctcctcttac tacagtgtga ccccttttta
    51601 ttaaactggt tatttttata tcttctttta atttaatacg tgtatcatca ttttttgaat
    51661 atactttcca tatcacttta tgacgataat tatctaattc ctttattaag cttctatcaa
    51721 tagtaaaact atctttagtt accacaatta aatcaaaatg gtctaacata tattgaaata
    51781 tttctctcat acttttattt tttaaaatat cctcaccaat aacctctata aatctacagc
    51841 ctttctcata catgcagttc aataaacttg tgatttcaga aaaataatca tttgtattat
    51901 tatctccatc tatttctata gtaaagtaca aaggagtaat gtatgattta tttcctgtta
    51961 tctcaaatac acttgatagt gtctgtatat catcttcaaa cgttaagtat acattctcat
    52021 aaaacataaa aaattccata actttttctt tcatattatg gatatttcta tatctattgg
    52081 ataatatatt tattatatcg tcagtagtat ttttcccatc acattcagcc agtataacaa
    52141 tttgcttcca acttaagcta tccacatttc cgatactttt tttatatcca tcttcttcat
    52201 cataaatagg gccaattata atgttttctt ttggccacag atgaacccta gttccatctt
    52261 ttaatttcgg atatttattt tttacagact cataaaatct acttgtatca cccatgattc
    52321 tttactaccc cccatagttt aaatattcta tgctaaaaat agcactttat ttttattaat
    52381 tcaacaatta ttcaaaattg gtcattatat atgtttaaat ggatttaaca tcaaccttat
    52441 ttatatgtta ttgatgttta tatatgttat ataaaatctt atttctcata cttcaatgta
    52501 atcttaacta tctctggttt acaattgagc ctaattaaaa cttttgagcc tcctaagcct
    52561 ttatttaaaa tcatagtgct attatctttt tcatattttc cccagtcata cttaggaaag
    52621 aactctctat ttggagataa tacacctcca acaaaaggta ttcttattat acctccatga
    52681 acatgtcctg ctaaaaccaa atctgctccc cattttgcat atttttcaaa gaaaaatggt
    52741 gtatgtgcta aaagaatgtt atattcattt tcattaactt tacctagatt gtcttctaca
    52801 aaattgtcac ttagttctaa ttttttgtta ttagcaaata aatatggata gcattccaat
    52861 ggtataatta aaccatataa attaagatgg ctattacctc tatatattct tacagattca
    52921 ttttccatat ttattatttt ctttgaatat agttccttaa aataatcctt atatagatgt
    52981 ttatactttt ttattaaaga tttttgttca tgatttccaa ttatatgata tacttcatat
    53041 ttttcaacca aattatctat taaatctaat gcaacttgaa aatttttatt ttctccatct
    53101 actaaatctc ctgttataaa tataacatct gggtttaatg attcaatttt atctatcaat
    53161 accttattat ttttatcaaa caccttatta tgtaaatcac tcacttgaac tattttaaag
    53221 ttatcaaatt cctttggaat ttttttgttg aaaatagtat attctgtcgt ttctatatgc
    53281 tgagtttcat acttatacag aatatacaat aatattaata ctaatactgt cattgaaacc
    53341 tttataatca tattctcttc ctctttattc tattttgtta atattattat aatgctttta
    53401 tgtttttttt aaaacaataa catcatcaca tacatataaa tcttttcgac attataaaaa
    53461 taagttatcc ttttctaaaa ttttcattta ttttagaaaa ggataactat aaaattatct
    53521 ataaacctct atattgtgaa atatagtttt tcctttattt aaatttttat atatatgctc
    53581 taaagttcca tcaaacctaa tagaatgtcc agactctaaa atataagttt tcccatccac
    53641 attaaggtca agtttacctt ctattacgag cacatattct tctaaaacat gactatgtgt
    53701 agatgaaaca tgagtacagt tttcctcaag ctcaattata aaaagttcaa accttctttg
    53761 agggctaaaa gggtatattg gatataactt catgttccct ttttgctcaa caataggctc
    53821 tacttcttct ttctttataa cctcataggt atttgtattt tcatttaata gtgtagtaaa
    53881 atttatttta aatccagatg aaattttcca taaagtagat actgtcggac ttgattcacc
    53941 tctctcaatc tgtccaagca ttgctttact gacacctgtc atctttgaag ctttgtctaa
    54001 acttatgccc atttcttgtc tcaataacct taaattttct cccacattag gaaatttatt
    54061 atccatgtta aaatcctcct gatagctatt tgtattctat gatttttact cctgtaagtt
    54121 ctactatctc ttttaatttt aacatatgat tgttacaaat ttcatcaaat tcatatatat
    54181 aatcttcaag ccctaatgcc ttatatttat cgattccaaa tttatgatat ggaagtaact
    54241 ccatcttgcc tcctggaaca ttttgtttaa caaataatgc agtatttctt atattttctt
    54301 cactatcatt tactccataa ataattggca ctcttataac catagattta ttcaactttg
    54361 aaagcctaca tatattatct aatataattt tattacttac tcctgtatat tctttatgaa
    54421 tattatcatc catagatttt atatctacaa atatgtggtc tattttctca aatacatcat
    54481 ttaccttatt ccaatcaaaa tatccacaag tttcaattgc aatatttatg cctttatcat
    54541 aaaaaatatc aactaattct cttaaaaaat caatctgaag tgtaggttca cctccagaaa
    54601 aagttatccc tccattagat tcaaagaaaa atataaaatc tttttcaact tcctctacta
    54661 tttcttcaac actcatttct tcggtcatta tacaaatggc atctttaaga cataccttga
    54721 tacactctcc acataaatca catttattat ttatttgcgt tctatcaaat agtgaactta
    54781 tattttgagg acaaacatct atacatctat tacaggatac acatttatct ttcataacac
    54841 ctaattttac tatgttactc caggaatctg gattagaaca ccacttacat tttaatttac
    54901 atccttctaa aaatatagta gtccttattc catctccatc atttactgaa aaatgttgta
    54961 cttttacaac tctacctttt ttactcataa tctacctcat aattaaaaat tgctatgtga
    55021 atttctagct attatagcat cttgcatttc tcttgataaa ttaacaaatt gagtactata
    55081 tccagcaacc ctcactaata aatctttata ttcttctgga ttttcttgag cttttaatag
    55141 agtatcattt gatatcgtat taaattgtac atggaatcct cctaaagcaa aatatgattt
    55201 aatcatagat gttagatttc taagtcctct ttcagtttcc accaaatctt gattcagtct
    55261 taaatttaga agtgttccac cagtatgaac atcatgattt atttttgatg cagatttcat
    55321 tgctgctagt ggacttgttg tatcagtacc aacaaatgga gaaactccct ctgtcaaagg
    55381 ttttgtcgct tttcttccac aaggagttgc acctactatt tttccagttg gtacatagtt
    55441 tgatataccc ataaatgcac tattaaattt agagccaaaa atatccttgt attttctagt
    55501 ttcagtataa taaaaatcag aaacttcaat tgctaatgaa tctacataat cattatcatt
    55561 tccatactta ggaacatcca acgcaagttt tcttaattct tcataacctt cccaatcatt
    55621 atttagtgct tttgttaatt cttctaacgt agtcacctta tcctcaaata ctaatttttt
    55681 tatagctgcc aatgaatttg aaactacacc taaccctata ccagttaata caggacctat
    55741 attatatttt gctcctcctt tacttaaatc aactccctta tccaaacatc catctacaca
    55801 agtagataaa aatggtcttg gtaccatttc tttatgaatt tgttgagcaa cagtagttcc
    55861 tataacagaa ctttttacaa gatatgctaa ttgctttaaa aactcatcct taagttcttc
    55921 aaaagaagta aattctgtga tattttttgt atctaacccc attttttctc cagttaatct
    55981 actcttacct tcattcatag catattctaa agctgctgca aaattgatat ttacggctga
    56041 agtccactgt cctgtttttc taaaatgagg aactacacaa ccacagttac tccaatctct
    56101 agcatcttct gcatcatatc catcttgtaa taacatttgt gcccctgcac tatcgctgtg
    56161 tatagcaggg aaaccagttc cttgttttac caatttagca actgccatta cgaaattatc
    56221 tggtgctcct tgactcactc taacacttaa tcctggctgg tgagttttta cactttctgt
    56281 agcctttaaa cacatatatg agatatcatt cgttccatca gtaccatttc tctttttacc
    56341 accaacagta agattttgga attgattata tcctgcaaag taatttgctg tatttgctga
    56401 tatagtccat acccattctg ataacttaat ccaaagtgct tctatcaatt cttgagcttc
    56461 tgattcagtt attttacctt ctctagcatc attttcataa tatggataca tatattggtc
    56521 aaatctacct aaatttaatg ccaatggatt ttctgataga ataccaccta attgaacaaa
    56581 ccatacaaat tgtattgctt cataaaaatt agttggaggg tttgctggta cattcataca
    56641 tatctctgaa atttttataa gttctaactt tctcttttca tctgtttctt gcttactcat
    56701 ctcttttgca agatttgaat atctttgtgc caatgtcata ataccctgtg caactataga
    56761 aacagatttg taaaaatcta tcttttcaat attttctgaa actgatatat ctaattcttt
    56821 aattttttta tctgcatctt ccttaatccc tttatatcct tttacaaaca atatatcttg
    56881 ataatctggt gttacttcac caactgcctg tctccactta gaatcattat ctattattcc
    56941 tgtatctact aatatcttag ctgtatcttc tgggattctt gctaagaaaa cttcttccaa
    57001 agattttccc ttccaatatg gaaatatttc ttttcttata aattctattt gttcttttga
    57061 tattacatat gggtcttgag ttcttttatc aaaagtatcc atttctttgt ctacccattg
    57121 ccaagaaaac tctggtgtca atatccctgt tcttctaaat tttcctgctg tacctactat
    57181 taattcattt tcaaagattt ttataggaag ttctttacat gtctccaaaa aagctttagc
    57241 tcttcttata cagatagctt ctccctcagt ttttttatgt gattctgtaa aaattctagc
    57301 cctttcacaa catatcatag gtttagaatt aatatattct tgtctaaatg cttctattct
    57361 attagtttga ctcatttaaa atcccccttt ggataatata tactacattt ttgtatgtta
    57421 taacatactt taattatatt atactgtcct ttagcataaa atggaagcaa aataacttat
    57481 tttttttcat ctaccttgaa cacctatata ttaatacttt ttttgttaaa tttataaaat
    57541 ctaaaaaaat tttctatgat atattttcta tattgttata gttatttcta atatactaat
    57601 gatttgaaat atcaaatttg tatactagac ttagttttaa aaccttagtt gattgtttaa
    57661 ctttgttaac ttcaacatgt atatccttca tataaggaag ctctacaaca cctactccct
    57721 tattaaagtc ttcttcaacc atagaaaaaa tttctttctc agttaagttc gtattagtca
    57781 atgatttaaa taaattgtat ataaatttaa tatatttatc actctcagat aacttatcat
    57841 cttctggata ctctttagca cagagaatat ctattgttac ttttccttct ttatcgtctt
    57901 cagacataga atagaataaa cttcgaatat ttttttcagg tggtttttca gcaccattta
    57961 tagatactac aaagctacca tctgattttg agttagtaat actataacca ctgtctatta
    58021 tagctttcga aaatctttga gataaatctt tttttacttc ttctctacct ttcgcatcat
    58081 taatcttttg tgagtaatta ttaaatagct cgatagattc atctttttta tcttcaactt
    58141 gttctttcaa tgaatcttta ctaatttgtt ccgagctact taagttctta tcagatacag
    58201 gagacataca tcctaccatc aatgctaagc taagatttaa agccacaatt attgccactc
    58261 tatttatatt cctttttact ttcatacaca atcctcccat cacactaacc gctctaaagt
    58321 attattcatt gcactagaat atatttatat tttctttata ttaaagattt attattcaat
    58381 acatatcacc ttcctatcca attcttataa cttactatta gtaaaattat aagctcctta
    58441 atacagaaat aaggatgtta tcttttgtta ttaaaagata acatccttat ttttttgtat
    58501 caatacatat gtcccaactg gtaacttaat cgccccaact gtcagtatct tggtccaatg
    58561 gtatatatat atttagtata aatttatcga tactattttc aaataataat tctccattat
    58621 atttttgtaa agaagattta atactctgaa tacctattcc atgtacgaac ttatccattt
    58681 tgtttgttaa caatatattt ttattaaaag taagctgatt tagtttacta ttttcacatt
    58741 taagtacaaa aaatgacctt gttatagttc ctttaattct aatatattta ttaatatttt
    58801 catccactat tttatcacat gcttctattg cattatccaa aatattagaa aatatgcttg
    58861 acacatctat aggttttaca aaatttactt ttgaaaaatt tatagagcaa gtaaatttaa
    58921 ttcctttttt taaacatact tcactctttt cgttgattat aatgtctaat atcatatttc
    58981 ctgtattata gatatattta aatgttttta tttcatcttt taaattgtta acatattcat
    59041 taatttcttt gctattgttt ctaaggttgt ctatacaata aatgtgatta tttatatcgt
    59101 gatatagttt cttaactcgc atatgagatt cttgaatact taaataatgc gcatattgca
    59161 tatctaactt attttttatt aattcagctt gagattttaa tctattctct tttattattt
    59221 ctttaaacaa tagtataaga aatatactac tagctcctac caattttgga ttaacaaaat
    59281 tatcaaaagt atccttctga atgtaatgac ccaaaccgtt ctaatttttt taatacataa
    59341 tttacaagca tattcattgt attaactaat aaggcaaata ataaatatac ataatttctc
    59401 ttatcttggc taattaattt tatagacttc ctaaaaaagc taaatataag atatataaga
    59461 ataattatta aattactata tatctgaatt tttatagatt ctaagtgagg tactaataag
    59521 gattctttag atgaaataac attacctgta attgcaaaaa atagtattct atatataaca
    59581 gcatccaagc atttataata tacactaaat agataaaaga aaatggaata cttaatataa
    59641 cccatcttat aaaaataatt atagtatcct ccccaagcaa taaatgctaa tatataaaat
    59701 aaatagttta tattactaaa gtaatttcct tcattaaata atctacttat ctgaattata
    59761 atataaataa gaaacaagac aatatagaat actatttttt catttttttt actatccttt
    59821 ttataaatta aaattccgtt cattatagaa aactgtataa atatagataa aactactaat
    59881 agctcagtca taaagtttag catattgtat caccaagaaa ttttaagaat ttctccttaa
    59941 cttctttata tctatatcta ctaataggta cctcttctcc actttccagt attgcagcat
    60001 taggctttat attttcaaca tatcttaaat ttactataaa acttttatgg catcttataa
    60061 atttatatgg atttaattct ttttctattt tacctaaact atattttata tcaaaattct
    60121 tgtttattgt gtgtattagc atatcttttt tttgaacttc tatatatttt atttcattta
    60181 aataaatctt atatgtatta gacttatttt ttattgtaat atgactttcc ttatttattt
    60241 caatatcttt gatacaagtt agcacatgtt tttttagttc ttccaactct attggcttta
    60301 gtaaatacct ataagctctt acctcataac cttcttgcac ataatctatt aatgaagtta
    60361 caaatattat ttccatttca ttgtctactt ctctaatctt tcttgcagta tccattccat
    60421 ttaatttttc catttttata tcaagaagta atatatctat atcttttaaa ttactttcca
    60481 gtaactcttc tccagatata tacttttgta tctcatattc tatacttata tcttttaata
    60541 tattctcaag gtaatcttct attaaaagtt gtgtttcttt ttcatcttca caaactgcta
    60601 tcttaatcat aaaatgcctc cttacataat ttctagttaa tacaattaaa atatttataa
    60661 tatatttaaa gataattttg tatatctgat actttaggat aacattttta aattattttt
    60721 atcaatactc aatcccacac aactattatt tgatatttca atttaaagac agaaattact
    60781 ataattaaaa acagtactta ctgacaaaag aagatgtctt aaaatattaa aaaaataatt
    60841 ttaggacacc ttattttatt tatttaaact ttttaagcct taaataatac attaatctcc
    60901 tatataattg tactacatat aaaatcaaca atataatctg tagtacaata gcagttacag
    60961 atgctccata accactatat aaatttctta tttcaatatt caataaatat ataaatatta
    61021 ctactaacat aaagaataca aacatattca taaataaaac tgactttact ttatttgttt
    61081 tatcagaatc actatataat ttaggaggta tgtctttgta ttctttcgac catatatacc
    61141 atgcagatgt acccataaaa ctaccaatac tcctaaatac aagattccag ccatttgatt
    61201 tatgttcatc aaaataatta ttctctacat catagtagta atcaacagtg tattttattt
    61261 ttcttggact tcccttatga aaataaaata ttcctccaaa tttatttatc ttatataaat
    61321 tatatccttt agcttcaata tcttctaacc atttctctat cttatcaggt gaactatacc
    61381 agtcaaattt gaattttatt ttcttttcca tagcactcat tctccaaaat gatattttta
    61441 aatagcttaa aaatctattt aaataattat atcattttga tactaatttt aggttacaat
    61501 catatattta aaaagctatt taaatagtaa taaactaata gccataacac ccattcctgc
    61561 aatcaatcca taaatagata aatgatgttc cccgtactct ctagctgatg gcaataattc
    61621 atctagtgat atgaatacca taattcctcc tacaattgca aatacaattc caagagttat
    61681 atcatttaaa aaatttctaa gaagtacata tccaataatc gctcctaaag gctcagacat
    61741 accagataaa aaggaataat aaaaagcttt ctttttatct cctgtggcat aataaactgg
    61801 tacagataca gatatacctt ctggtatatt gtgtattgca attgcaattg ctatcggaat
    61861 agctattgta acatcatcaa gagctgatat aaatgtagct aatccttcag gaaaattgtg
    61921 aattgctata gccattgctg taaatatacc tgttctcaaa agagacttat tttttttgat
    61981 attcttttct tctatattct ccattttctt tatttcatgt ggattttctt cttgtggaat
    62041 tagcttatca ataacagcaa tgatggccat acctataaaa aaagctatta cagttgacca
    62101 acttcctaat ttctctccca tagattttgt caaagcatct tgtgctttag gaaaaatttc
    62161 tatcatagaa acataaatca taacaccagc agaaaatcct aagcttatag aaagaaactt
    62221 agtatttgtt tttttagcaa aaaatgctat acaacttcct ataccagtag ataaaccagc
    62281 caataatgtt atcaaaaatg caaatattac attattcaaa taaatcacct tctttttaat
    62341 tgatattgat tatcattttc tttatgctat catatatatt ttatatagtc aatatttata
    62401 aaaaatatac acaataaaaa agcagagata taaacttagt ctctgctttt tctttatagt
    62461 atattaaaat tatttattta agaaatccac tgcatattgc acatataaag cagttccaat
    62521 ttctagagca tcttcatcta tattaaagca tccatgatga tgaggataac aagcaccttt
    62581 tgactcattt ctagctccta caaatgctag tgcaccaggt gctatattca taaactcagc
    62641 taaatcttct gctcctgtta ctttttcaaa tagtgttata cagtcttctc caaatagctt
    62701 aattgctgca tctgtagcta tctttgaaca ttctttgtca tttataactg ctggtgttaa
    62761 gtacccatac tctaattcag catctgctct atatgcttct gctgtagatt ttgctatacg
    62821 ttctaaaatt ccaggaattt gctttctaag ctctggatta aatagcctta ttgtaccttc
    62881 taagatagct tcactcgcaa ttacattaaa tctagttcca gaatttaaaa ctccaacact
    62941 cacaactaat ggttccagag gacttacttc tctactaacc atagattgta gattcataac
    63001 tattgctgaa cttgcaagca ctgcatcaac accttgatga ggaagtgaac catgaccacc
    63061 tctacctttt acagtaattt taaagaaatc agcagaagcc attcttggtc cttcttctac
    63121 cgatatggtc ccagattcta catcagtcca aagatgtatt ccaaacacac tgtctacacc
    63181 ttccatagca ccatcttgaa tcatagctct tgcaccttta ccaacttctt ctcctggctg
    63241 aaaaaataat tttacagttc cattgatact atccttaata tcatttaaca cttttgctgc
    63301 ccctagaagc attgatgtgt gaccatcatg accacaagca tgcataagtc cttcattttt
    63361 agatttgtat tctacatcag tgcattcaac aacttgtaga gcatccatat cacccctaag
    63421 tgctacagtc tttcctggat tagctccttt tatagttgca attacacctg tacctccagc
    63481 tgatacatat ggtatcccta ttttatcaag ttcttcttta actctctttg atgttctaac
    63541 ttcttccata cttttctcag gattttcatg aaattccctt ctaagcttaa ttacatagtc
    63601 tttataactc ttcgttattt ctttaatatc cattccatac ctccaagtag tacacattct
    63661 tcttatttaa aatacttttt atcacttaaa tactatatta gatttacaaa gatacctgct
    63721 attactacag aagctattgt aactgtagta aatcctccaa caagcatctt tggaagcatc
    63781 ttatccatta aaaattcttt ttcttctggt gtttcagcca aagcctttac tgcctcttca
    63841 gttaatatat agtttggtgg aaaaccataa agtgatgtaa gcgaaactga aaaagccatt
    63901 tctttacttt cacctaaaag cttacctact ataatagaga atattcccat acctattaca
    63961 ccaaatatta tgattcctac aagaggaact gccatttcaa caagcattgc tggtgtagct
    64021 tttgctaacc ctgcaaatat aaaagccatc aatgatgtca ttaaccatcc aaatgaacca
    64081 gatttattaa gtggttttct atcaactatt ccaagttctg atgctattac accaaatatt
    64141 aaacatataa catatcttga tacaacttca tttgtaagat ttgtaaattg tactgctaac
    64201 catgctacaa atcctaatct aagtattatc atatatgtcg tatcatattt ttctggtaag
    64261 tctggtatta tcttaaatct gcttttttgt tcaggtaaat ccttaacttt ttcttcatct
    64321 gtatttacag ttacttttcc acttctaaag tctgataaaa gtctttttcc ttctttttta
    64381 agcattaatg ctgtaatagg ataacccgca aatccttgtg caacatacat caaaattgca
    64441 agtactgata aacttgcaag accttttgct gtagcagcat ctgccatcat tatagatgca
    64501 actagccctc cagtaagagg aggtgtagct attactgctg tatcccatcc aaatactact
    64561 gtccctaatg ccaaagcacc tacacatatt cctactattc ctgccaaggc tattactatt
    64621 gttttccatt gacctgctaa ttccttaata ctcatcatag ttcccatatg agttattaac
    64681 aaaagcatac ctaattgagc taatggtgtt cctaaagcgg ctaagtccac aatattttct
    64741 gggaaaaaag tccaaaatcc tactataaat ataattgctg atacaaaaac cgacggaaca
    64801 aaagctttag tttttgctga aactatatct cctatcgtca gtgatactaa aactagacaa
    64861 aaagctgtta ttgcctccat acaaaatccc ccctcataat aaatatattt ttataaaatg
    64921 tatgtatagt tatccattat tttactactt ttgaaaaaca tttcaagtca aaattcgatt
    64981 tttttcataa aattcgataa attccataaa ttaaagttag ttttctaaat tattcttttt
    65041 gtaaaaacgt tatcccgacc attggctatt taactctatt cttacaaaga gaattaaatg
    65101 taatttgcaa aaaataaaac ataggataca ttttattcat aaaacatctt ataaatataa
    65161 aatgaattgt ctttatgaat cttaatatat cctatgtttg taagtttaat gtgtatgtta
    65221 gctatggtgt aaaatttgta tactagttat tgtgtaacct actatatact ccatttttgt
    65281 ttactagctc ttcatgagta cctctttctt caattccttt atccgtcaat actatgattt
    65341 catcagcatt ttttatagta gtcaatctat gtgcaacaac taaatttgtt ctatctttac
    65401 ttaattcctc taaagactct tgaatttctc tttctgttac attgtctaaa gcagatgtag
    65461 cctcatccaa tattattatt ggaggattct ttaaaaagat tcttgaaatt gaaattcttt
    65521 gtttttgccc acctgataat tttacgcctc tttctcctat ataagtatca tatccatctg
    65581 gcaaagtctc tacaaagtca tgtattctag cttttttagc agcagctatc atttcttcat
    65641 ctgtagcatt agggttccca caaagaatat tatctcttat agttcctgta aataaaaata
    65701 catcttgttg aactatacct atattttttc tcaatgattt taaactaaca tcttttatac
    65761 tctttccatc aatttttata tcacccttat caaattcata aaatcttggc aatagattac
    65821 ataatgtagt ctttcctcct ccagaaggtc caactaatgc tatagtctta ccagctttta
    65881 tagataaact taaattatcc aaaacttgag tcttatcttc atatgtgaaa gatatatttt
    65941 ctatctcaat gtcacctttt acattttcaa gttcaatagg atttttagct tctttttgat
    66001 ggtcttgttc cattatctct atgaatcttt taaaacctgt cataccattt tgatattgct
    66061 ctgtaaagtt tattaatttt ttcataggtt gtataaacat ttttacatat agtatataag
    66121 cagcaaaatc acccaaagta atctttccta tataagtaaa ataaccacca gctattacaa
    66181 ctacccactc taagatatcc atgtataaat tcataccaga gtaatattct gccattactt
    66241 tataagaatc ttgtcttgct cttctaaata atttattgct tcttacaaac ttatttaact
    66301 cttctttctc agtacaaaaa gacttagtta ccttcatacc tgctatacta ttttctaaag
    66361 tagcatttac atcacctata gtaactctac tcttcataaa agcaatattc atcttatctt
    66421 ttttagctat tgcaaaccaa attataaacg gtaatatagc aaatattata agcgtaagag
    66481 gaatatttat atctataagt ataaagaatg aaccaaccaa cattactata gaaatgaata
    66541 aatcttcagg cccatgatgt gcaagttctg ttatatccat taaatcattt actattctag
    66601 acatagttac tccagatttg ttattatcaa aatacgtatt tggaagtctt tgtaagtgtg
    66661 taaatacctc atttctcata tcagcttgca tatctacacc aataacatgt ccccaatact
    66721 gcataaaata gttaagccct gcttttgcta taaatataag tataagtgct actgcaaaaa
    66781 ctactaacat tcttaagttt ttatttggaa caacgtcatc cattatattt ctagttatca
    66841 taggataaac taaatcacat aaggaaaata gaaaagctgc tataaggtct agtgtaaaaa
    66901 ttttcttata tggcttataa tatttgacaa attgttttat cataagtatt aaatccctcc
    66961 tttattagta cttctattag tacttctaaa aaatacccat aaattagtat aacataacaa
    67021 taactttaaa tcttttataa acttacaaag taataacaat ctttttcata aaattgaaga
    67081 tttatatcat atagttgtat acttgaaaca acataaaaag agctatatct ctatatttta
    67141 aattttataa agatacaact ctatatttaa tactaaattt tttttataaa tacaaaacta
    67201 cttcaatgca tttaatatct ttttatctaa tgctgttgta tttatacctg ttatatatcc
    67261 tgttgccttt attgtaggta tatcataagt tgttggtagt atagtagtag atacgattaa
    67321 atcagcacct tcttgttttg ttgaacattc agcaatttta cattgctcaa cttctgcttg
    67381 tatattattt tctttaacca atctttctac tctgtcacaa actattgttg atgttgcgat
    67441 tcctgctcca caggctacta atattttttt catatattgt cctcctacaa tttctttaat
    67501 attttataac tcaaaataaa attgataaat ttcttatatc tttatttata taaataatta
    67561 tatcatttta tataagaaga acatcttaaa attattcttt taatatgttt tttatttcaa
    67621 cattgcttat acttaaaatt tcatttttta tacaagactc tatgtacttt gatatatttt
    67681 tataatcatt taaattaaac tgttcaatat tcaatttata ttctatatca ctgacaatac
    67741 ctatcaaatc agacttatta gataaaatat cctcatactt accttttcta gttacttcca
    67801 atcttttaaa tctataattt ttatatcctt ctatgataat caagtccata tcttcatata
    67861 attgaatcat tttatataat ggattcttct cttttagttc ttcaatatat gctactcgtt
    67921 tatctgatat tatacagact tttctcgccc cagattttct atgtttataa gtatcttttc
    67981 cttcctcatc catatcaaaa tcatgacagg tatgtttaat aactccaaca cgataccctt
    68041 ttttatttaa ttcttttatt atgccttcaa tcaacgtagt tttacctact cctgaatatg
    68101 agacaactga caatatatta tacataaaat tgtgtccttt cataaaacta tctaataaca
    68161 acatccacta attgattttc ttttattgtc tcattctttt taatcttaat catacaatta
    68221 cttcttagag ttgcagatag tctagctttt gcatctttct tatcatttag ctctacatac
    68281 aatttacctt cacttgtata tagttttcca cttacaaatc tatctttatt tgaaagttta
    68341 ttaaattctc ctttaaatat agcttttata tgatgtgttc ccttcttata tgttaaatac
    68401 tcaataacag gaactatcac attatcaaat accactattg ctgcatgtgg attaccagaa
    68461 agaccaaata atagcttttc tcccaatgta cctacataac aagctccacc aggtttaatg
    68521 tttatcttcc aaaataattc tgttgcccct atttttttga atacatctgg aattaaatct
    68581 ttatctccaa cagaaacacc acctgtggta attaaaaaat ctacactctg taacatattc
    68641 tttacctttt tttctaactc caatatatca tcagaacaat tgcatattgt atctgcttgt
    68701 attccaagtt ttaataatct tgaatataag gtatatctat tactatcgta tattttacca
    68761 ttctctaaag tctcatttat atcttgtagt tctgtacctg tattcaaaat tccaactttt
    68821 aaatcatcat aaacagttac tttggatatt ccaagacttg ctaatactcc aataccatca
    68881 tatcctatat tctgattttt attaataatc aattgcccct tttttatatc ctcaccctta
    68941 aatatgtaat tatcgtattc ctttaatttt atatttaaag ttatcatatc acctataact
    69001 gttacatctt ctttttttat tacacaattt gcaccctgtg gtatctttcc accagtcatt
    69061 atcttcactg cttcattttt atttaatacc ctattaaata catctcctgc atacaaagtc
    69121 cctataacct ttaaatttct attttcagaa tttgcattat atgcataacc atcatagggt
    69181 gacctatcaa atggaggact atcaattgtt gaatatatat cttctgcaac taccttattt
    69241 actgcatcta taagatttac tatcttgacc ctgtttattg gtaaaacatt tttttcaata
    69301 atttttatgg catcttctaa ttctatcatt ttaaatactc cccaatatat tttttattta
    69361 agatatttct atgtaatata ttatagatta taaaagttgg aaatactttt atcttatatt
    69421 taattataac taatagttat ctataagtgc agttcaaatt ttataaatct aataaaaaaa
    69481 ctgttcccag ctagaaattt ccttctatct tgaaacagca tcatatgctt tagaatattt
    69541 tattctaata aagttccttt tattattttt acaattttat cactttcaat agtttttata
    69601 gattttattt tgttatccac aatacttatt gtaagtacct ctttttttaa tattttgtta
    69661 tcttctttca atctataact cttaactact ttactacctt catgattata tgctgttaaa
    69721 tcataattcg catctcctac aatttcattt attctggaat cttcaacacc aacatactct
    69781 cccaggtcaa tatgtctaaa attttctagt gatacagccg atttttcatg gtcacttttt
    69841 tcaatcacta tactagattc agcatcaatg actgatatac cttgtgatga gaaagaattg
    69901 gtttcaacat tgacaacttt attttcgtta atatcaacat ataaagcact tccatctagt
    69961 tcatcatttc cagtatattt tggataaact aatgtcatat aacttgatgt atttatatct
    70021 tgcattgtat ttgattcttt taaaacttta ggttttatat aataaaatgt attataaggt
    70081 atacccatat tctttataat gtacttataa tctttattca taatttcatt aacatcattt
    70141 tgaacttttg tcatagtttt ttgctgtgtt gctttacttg ctacttgatt tttctcttta
    70201 gtattttctt tatgacatcc aacaagtata atacctaaaa tagttacaca aactaatgac
    70261 ctttttaact tcataaatca atcctccttt cttaaatttt atagtattta tctatatctc
    70321 aatttacagt tatctctttt ttcaataatt atatttatta tattataaaa taattatata
    70381 atatttattt gaatttaata agattaagaa gctaattgta atttttctgt aataaaaaac
    70441 actttaaagt tgaattttga aaattcaatt tacgatattt ttattgtaag taccttacaa
    70501 cattatacca aattattcct aaacaatcaa aaactccatg aatatttaaa ggttttctat
    70561 tttaaaaatc acctttgact attaatttat caaatatgtt ataatagttt tagataattt
    70621 aaggaggatt aaataatgga ttttaaacaa cttgaagtat ttgttgctgt tgcaaaacat
    70681 cagagttttt caaaggctgc aagagaactt tttttaactc aacctactgt aagcgcacac
    70741 atacagaatt tagagagaga gctagaaact gttcttataa atagaagcaa caaagttatt
    70801 acactcacaa aatctggtga aattctttat gaacatgcta tatatatatt gaataattgc
    70861 aaacgtgcaa tttatgatat aaaagaatat tcaggaaaaa tagaaggtat catagatata
    70921 gcatgtagtt ccatacctga aacttatatt ttacctgatt ttatgaagag tttttctatg
    70981 agttatccag atgtaaaatt ttccataagc cattatgatt ctcaatatgc tatatcagaa
    71041 attttaaatg aacgaataag ctttggatta gttggttcta aaatcaataa tcctcaaata
    71101 gagtacctag acttattaga tgatgaactt gttcttatca ctccttcaga ttttaaaatt
    71161 gataataaaa ataattgtat cgatatagga gaattggctt atctaaattt tattatgaga
    71221 aaagaagggt caggtacaag aaacttaata ctaaacacac ttagtaaaaa taactttcca
    71281 gtaagtaaat taaatgtaat cgctcatgta gaatcaaatg aggctattaa ggaaatggtt
    71341 agattaggtc ttggagtgtc ctttatatcg tacatttcag ctatcgatta tctcaatgca
    71401 ggtaaaataa aatgttataa aataaaggat gttgatttta ctagaaagtt tttcttcatt
    71461 tactctaaaa agaaaacttt ttccccatta gaagataaat ttctaaacag attatgtgag
    71521 tatttcgaaa ttataatata ataactttaa taaagtagtg cttaattttg agtttataac
    71581 taattaataa gctaattata actatcacat aaatgcttat aaattattat gaaccttctt
    71641 tcttttttat ttaatttagt caaaaaatcc ctcgttcaaa tgaacttggg atttttttat
    71701 ttatactact ccaatagcag ccaatactat acaaataatc ataactgaaa ttaatatcgt
    71761 agttgtcttt accttttttc ctaataacca atacataata ccgaatatca tagctggaag
    71821 catacatggc attatttggt ctaagtaagt ttgaataggc tctgcccatt ctccactacc
    71881 tacattaaca ctaagtttta acattacatt agatgcagtc ataccaccaa ttaccataag
    71941 ccctaacatt gaagctgctt ccataacttt tgaaactata cttgaaccac tcacatcttt
    72001 aaagaattga gttccatact taaatccttt atctagacat atatagcgta taataaaatg
    72061 aggaacattt attattaaaa ggaataaaat cggtcccatt atgttaccct gagaagctaa
    72121 agctatacca accccagctg ctataagctt taatgttccc cagaaaaatg aatctcctat
    72181 cccagaaact ggacccatta aacttgattt aacagcattt atactattag catcaaattc
    72241 tttattcttt gcattttctt cttccatagc tccagatata cctaccaaaa gagtacttat
    72301 atgaggagta actgacataa attctaaatg tctttttaaa gccttagatt tcttttcttt
    72361 atcatcttta tataatctac ggattattgg tgccatcatg tatgcatatg ctatattttg
    72421 catacgttcg tagttccaag atgaatctaa tgtcaatgac cttctaaaac atttccaaaa
    72481 gtctttcctc gttattttac tatctggtga aattgtctct aatttcttag aactcgttgt
    72541 catctactat tacctccttc tctcctacaa agttaacata tattaaagct attgctaatc
    72601 caaacaatgc aactcctgtt actgttatgt ttaggtaagc aaccaatata aatcctaata
    72661 ataaaaatgc tactgttttc ttattaagca tcattctagc taacatggca aaacctattg
    72721 caggaatcac gcctgctgca acatccattc catgagttac atatgatggt ataaatgaaa
    72781 gtattttttc tattactgga acacctaatg aaaatcctat acctactatt attgctaata
    72841 ttaatttatt tcctatacca aataataatt gcacacgttc tacgccttca atattcccct
    72901 cttctgcata acggtcagca atatgagctc cccatgtaag tactactgtc attaagaagt
    72961 tatctataag caatactaga gttgctatag gaactgcaag tacaactgct gctgatacat
    73021 cttgacccgt taatattaca aacgctgtgg caataataga accagacaca aaatcaggag
    73081 gattacttgc cccaacagga aatgcaccca taaatactaa ttctaatgtt gaacctatta
    73141 ctatacctgt ataaagatta cctagtgcta aaccaacaag tggtgcaaca actataggtc
    73201 tattatgcat tgtagaacct acaaaactat gcatatatgc aaagaatgca attagagtta
    73261 tcaaaataac ttgtgtaaat tccattttca tccccctaaa attatattaa tttcattaca
    73321 tttgtagcat catcatcagg aactaattgt acaaatacat ttacgccttc tgaatctaac
    73381 tctttaatca tctttatatc atcttttgat acatgcactg ctttagatat attttcacga
    73441 ttatcatctg attttgttcc accaaggttt atagatttaa tagcttttac ctctttagca
    73501 agtctataaa catcttctac agattcacaa agtatcaata agttgtattt atcagtaaca
    73561 cctgagttaa gtgcttttat agaatctgct atgcttttca ttactaactt aacattagat
    73621 ggtttagcca ttttaagtcc tgccattctc aattcatctt ttaataagtc atcacttgct
    73681 atcaatatac agtctgtgct taaaaacttt gtccatgtaa atgctacttg tccatgtata
    73741 agtctgtggt ctactcttac taatttaatc attataattc ctccctaatt tttttgattt
    73801 catccatgaa tgtttttacg tgttctacac taacatcccc aaaatctttg taattatctt
    73861 taaagtaact tcctattatt gccatatctc ctaatttaag ctgttttttt gcattttcta
    73921 atgtaactcc agcagctata accaatggga agtctcctaa atttttacga aacagttcta
    73981 ttttttccat tgatgtttct tgacctgtag cattttctgt aactgctata gcatcacatc
    74041 ttgtcattcc tatttttaaa tcttcttcaa catcattttc tgataatact ggctgatatt
    74101 taaaacgtac gcctccaatt aaatacgctg ggcattttga acgttgtaat ttaaaaaact
    74161 cttctaaagt agcttcatct ctaggtttaa catgacctac tacagaatca acctgtatat
    74221 aactagcatt atattttgtt gccagttcaa atcccatagt atcaacattt aaacagttta
    74281 ctccatatgg tattgataaa ttagcttttg atacatattc aagtattctt tctaaatcat
    74341 aataattacc atagtaattt tctaacatta ttccatctac accattttct tcaaagattg
    74401 taatctcttt tttagctctt tcaaagatat cttctggagt atctcctttt aagtgtatca
    74461 ttgctataat tggtttttta cttttaaata ctgataatat tccctttttt ttcataaact
    74521 aaaatacctc ttcttcttct ttaatttgta atgtcacgct ttgtattcct cttctagatt
    74581 cttccactaa ttggtctata tcttcaactg ttaattcatc agaaaacctt gtaagaagtt
    74641 ctaataccat attcaaattc attccagaaa ataacctaac attatgtaga ttagttaatc
    74701 ttaacattgc tgtacataca cttgctccat ataagtcaga taaaattata tattcacatt
    74761 ctttatcttc tgatattgtt ttttctaatt ctaaagcaaa atctgaagca ctttgtccag
    74821 ggtacaaacc atacgaactg acacaatctg ctaaatctcc tactatcatt tttactgcat
    74881 ttaaaagccc ttttgaaaag tctccatgag atgctaatat tatctttttc atactcatcc
    74941 tcctactaaa ttatagatta tatttctgct gatactgcat atttgtactt atcacttctt
    75001 ataaattgct cattgtaata taagacatca ttattttctg tatacaccga ttgtcttact
    75061 ttcaatagtg gcatattact tgcacattta agatggtcag aatattcttt gtttgaatgt
    75121 tttacttcaa atatattttc tactcgttta ggtaatgtgc ttatattatt agatataacc
    75181 tccattaaag atttatcttc taattcttct tttaataaaa acatgtaatc tattctaaaa
    75241 tagtcaactt caaaaataac aggtacttca tctattaatc taagtctttt aatacatatt
    75301 accttctctt ctagttcaat acttaaagct tcagctactt gcttatctgc tttttttatt
    75361 aaaacagaga ttattttggt acttggtttt gcattaatac gatgacaaga attagtaaaa
    75421 ctatttcctg cacatgttgc ttctattctc tctggtattg ttacataagt ccctttacca
    75481 cgttttttac ataatatatc ttcttttaca agttcattaa gtgcatttct cacagaaatt
    75541 cttatatttt atttatgttt atttatattt atttatgttt ttatgagtac atttatttac
    75601 aacctatcta aaacaatttc tttattctat tttatttata tttctttatt tgtattttat
    75661 tttattaaac tgtcatggtg gcaaaaaagg tggcaaaaat aatttgccac ctaaaataat
    75721 ctttatttat tctttctctt ttaaattttt aaaatgttct tcgagttcaa ttaatttatc
    75781 ttcatccact tcatcatctt tactatgtaa cgctgatatt aaacttgaaa ttgaattctt
    75841 atgtatgttc ttcaaaaatt cctttgtttc aaatcttaga tattttttct cttttaccat
    75901 tgcattatag tgtgtaagtt tacctatttt ctctgtactt aaaaactccc ttcgctctaa
    75961 cctttttaaa actgtgaatg tagttgattc attccattcg tatttttctt ccattgcggc
    76021 aactaattcc ctagacgtta atacatcgtt agcttcccaa atatatctca taacttttaa
    76081 ttctgcttct ggtaatttac taattttcat agtcacaccc ctttatcgaa ttatgtatat
    76141 gttaaattct atctcaataa agttttccaa acaagagaat cacactaaat attaccatcc
    76201 aattttttcc atctcgaaat tattcttcta aatttctata atattcttct aatgcgtcta
    76261 gtttttcttt acttatagac ttatgtgtag tagtaagaga acgcattatt tttctgcttt
    76321 tgctagattt taacactttt ttcttaaaag cataatattc tttgttatct attattattt
    76381 tataatgaga ttgaaacttt ataatatctc tggccaaata acctttgtca actagccttt
    76441 ttagtagtat ttttattgta cttttatgcc atctataaac ctgtctcatt tcttttacaa
    76501 tttctttttt agtcactaga gaatcttttt tccataaata ctccattact attaattctg
    76561 ctcttaataa tttttctact aatatttctt ctttcaactt atgtcactcc ttcgattgtt
    76621 ctagttcaag ttaataataa gagcttttta agacaaaaac tataggtaat ttttaccaaa
    76681 tattctactg ttttagtttg ttttcttaga agaaaaataa ctttcatatc tatagaatta
    76741 ttcaattttc aaactatcct gttgaataat aactaattct attgtattat tttagttgta
    76801 gaataaaact aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaag
    76861 atttataaaa ttcttataaa ttatgccagc cagttgccaa aaagatattg ttctttttgt
    76921 ttttatgaaa gttttttagg tggctaataa tatatatact tagagataaa ataaaatata
    76981 tttctagcat tca
//
LOCUS       DAMJYC010000007        73566 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJYC010000007 DAMJYC010000000
VERSION     DAMJYC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.007"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(402..1124)
                     /locus_tag="PC472_000509"
     CDS             complement(402..1124)
                     /locus_tag="PC472_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2725872.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1183..1860)
                     /locus_tag="PC472_000510"
     CDS             complement(1183..1860)
                     /locus_tag="PC472_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2725873.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1888..2703)
                     /locus_tag="PC472_000511"
     CDS             complement(1888..2703)
                     /locus_tag="PC472_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2725874.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2929..3139)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3815..5881)
                     /locus_tag="PC472_000512"
     CDS             complement(3815..5881)
                     /locus_tag="PC472_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2725875.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5958..8987)
                     /locus_tag="PC472_000513"
     CDS             complement(5958..8987)
                     /locus_tag="PC472_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2725876.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(9040..10500)
                     /locus_tag="PC472_000514"
     CDS             complement(9040..10500)
                     /locus_tag="PC472_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2725877.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10592..10960)
                     /locus_tag="PC472_000515"
     CDS             complement(10592..10960)
                     /locus_tag="PC472_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725878.1"
                     /translation="MGNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKIDDI
                     INKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVKAL
                     FSIFVIVCFLIAFKYVVNPV"
     gene            complement(11136..11876)
                     /locus_tag="PC472_000516"
     CDS             complement(11136..11876)
                     /locus_tag="PC472_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2725879.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEAGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11897..12685)
                     /locus_tag="PC472_000517"
     CDS             complement(11897..12685)
                     /locus_tag="PC472_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2725880.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12773..12863)
                     /locus_tag="PC472_000518"
     tRNA            complement(12773..12863)
                     /locus_tag="PC472_000518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12827..12829),aa:Ser,seq:gct)
     gene            13122..13886
                     /locus_tag="PC472_000519"
     CDS             13122..13886
                     /locus_tag="PC472_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2725881.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14167..15282)
                     /locus_tag="PC472_000520"
     CDS             complement(14167..15282)
                     /locus_tag="PC472_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2725882.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15323..16849)
                     /gene="glpK"
                     /locus_tag="PC472_000521"
     CDS             complement(15323..16849)
                     /gene="glpK"
                     /locus_tag="PC472_000521"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2725883.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17142..18239
                     /locus_tag="PC472_000522"
     CDS             17142..18239
                     /locus_tag="PC472_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2725884.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(19064..19576)
                     /locus_tag="PC472_000523"
     CDS             complement(19064..19576)
                     /locus_tag="PC472_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725885.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLNDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            20129..21010
                     /locus_tag="PC472_000524"
     CDS             20129..21010
                     /locus_tag="PC472_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2725886.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKISEG
                     EIELPDPIIMENDKLMK"
     gene            complement(21323..22102)
                     /gene="hisJ"
                     /locus_tag="PC472_000525"
     CDS             complement(21323..22102)
                     /gene="hisJ"
                     /locus_tag="PC472_000525"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2725887.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(22647..22997)
                     /locus_tag="PC472_000526"
     CDS             complement(22647..22997)
                     /locus_tag="PC472_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2725888.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(23013..23429)
                     /locus_tag="PC472_000527"
     CDS             complement(23013..23429)
                     /locus_tag="PC472_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2725889.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(23448..23639)
                     /locus_tag="PC472_000528"
     CDS             complement(23448..23639)
                     /locus_tag="PC472_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2725890.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23790..24605)
                     /locus_tag="PC472_000529"
     CDS             complement(23790..24605)
                     /locus_tag="PC472_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2725891.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(24652..25590)
                     /locus_tag="PC472_000530"
     CDS             complement(24652..25590)
                     /locus_tag="PC472_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2725892.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVMISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(25587..26459)
                     /locus_tag="PC472_000531"
     CDS             complement(25587..26459)
                     /locus_tag="PC472_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2725893.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(26484..28412)
                     /locus_tag="PC472_000532"
     CDS             complement(26484..28412)
                     /locus_tag="PC472_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2725894.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(28524..28901)
                     /gene="gcvH"
                     /locus_tag="PC472_000533"
     CDS             complement(28524..28901)
                     /gene="gcvH"
                     /locus_tag="PC472_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2725895.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28988..31114)
                     /gene="cdhC"
                     /locus_tag="PC472_000534"
     CDS             complement(28988..31114)
                     /gene="cdhC"
                     /locus_tag="PC472_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2725896.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(31171..31977)
                     /locus_tag="PC472_000535"
     CDS             complement(31171..31977)
                     /locus_tag="PC472_000535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2725897.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(32027..33394)
                     /locus_tag="PC472_000536"
     CDS             complement(32027..33394)
                     /locus_tag="PC472_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2725898.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(33425..34369)
                     /locus_tag="PC472_000537"
     CDS             complement(33425..34369)
                     /locus_tag="PC472_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2725899.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(34389..35159)
                     /locus_tag="PC472_000538"
     CDS             complement(34389..35159)
                     /locus_tag="PC472_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2725900.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(35215..36600)
                     /gene="lpdA"
                     /locus_tag="PC472_000539"
     CDS             complement(35215..36600)
                     /gene="lpdA"
                     /locus_tag="PC472_000539"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2725901.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(36644..37525)
                     /locus_tag="PC472_000540"
     CDS             complement(36644..37525)
                     /locus_tag="PC472_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2725902.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(37560..38234)
                     /locus_tag="PC472_000541"
     CDS             complement(37560..38234)
                     /locus_tag="PC472_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2725903.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(38271..39143)
                     /locus_tag="PC472_000542"
     CDS             complement(38271..39143)
                     /locus_tag="PC472_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2725904.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(39235..39867)
                     /locus_tag="PC472_000543"
     CDS             complement(39235..39867)
                     /locus_tag="PC472_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2725905.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39972..41648)
                     /locus_tag="PC472_000544"
     CDS             complement(39972..41648)
                     /locus_tag="PC472_000544"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2725906.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41897..42676)
                     /locus_tag="PC472_000545"
     CDS             complement(41897..42676)
                     /locus_tag="PC472_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2725907.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42986..44905)
                     /gene="cooS"
                     /locus_tag="PC472_000546"
     CDS             complement(42986..44905)
                     /gene="cooS"
                     /locus_tag="PC472_000546"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2725908.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(45512..46423)
                     /locus_tag="PC472_000547"
     CDS             complement(45512..46423)
                     /locus_tag="PC472_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2725909.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(46539..47117)
                     /locus_tag="PC472_000548"
     CDS             complement(46539..47117)
                     /locus_tag="PC472_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2725910.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            47343..48266
                     /locus_tag="PC472_000549"
     CDS             47343..48266
                     /locus_tag="PC472_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2725911.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(48438..49580)
                     /locus_tag="PC472_000550"
     CDS             complement(48438..49580)
                     /locus_tag="PC472_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2725912.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(49598..51352)
                     /locus_tag="PC472_000551"
     CDS             complement(49598..51352)
                     /locus_tag="PC472_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2725913.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            51488..51694
                     /locus_tag="PC472_000552"
     CDS             51488..51694
                     /locus_tag="PC472_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725914.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(51699..52622)
                     /locus_tag="PC472_000553"
     CDS             complement(51699..52622)
                     /locus_tag="PC472_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725915.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52876..54576)
                     /locus_tag="PC472_000554"
     CDS             complement(52876..54576)
                     /locus_tag="PC472_000554"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2725916.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(55038..55451)
                     /locus_tag="PC472_000555"
     CDS             complement(55038..55451)
                     /locus_tag="PC472_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2725917.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(55542..57920)
                     /locus_tag="PC472_000556"
     CDS             complement(55542..57920)
                     /locus_tag="PC472_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2725918.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            58239..59690
                     /locus_tag="PC472_000557"
     CDS             58239..59690
                     /locus_tag="PC472_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2725919.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(59894..62407)
                     /locus_tag="PC472_000558"
     CDS             complement(59894..62407)
                     /locus_tag="PC472_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2725920.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(62630..63451)
                     /locus_tag="PC472_000559"
     CDS             complement(62630..63451)
                     /locus_tag="PC472_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2725921.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63969..64445)
                     /locus_tag="PC472_000560"
     CDS             complement(63969..64445)
                     /locus_tag="PC472_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2725922.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(64479..65057)
                     /locus_tag="PC472_000561"
     CDS             complement(64479..65057)
                     /locus_tag="PC472_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2725923.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(65161..67761)
                     /locus_tag="PC472_000562"
     CDS             complement(65161..67761)
                     /locus_tag="PC472_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2725924.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTNKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(68214..68897)
                     /locus_tag="PC472_000563"
     CDS             complement(68214..68897)
                     /locus_tag="PC472_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2725925.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(69117..69707)
                     /gene="zapA"
                     /locus_tag="PC472_000564"
     CDS             complement(69117..69707)
                     /gene="zapA"
                     /locus_tag="PC472_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2725926.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(69732..72125)
                     /locus_tag="PC472_000565"
     CDS             complement(69732..72125)
                     /locus_tag="PC472_000565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2725927.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(72142..73161)
                     /gene="pheS"
                     /locus_tag="PC472_000566"
     CDS             complement(72142..73161)
                     /gene="pheS"
                     /locus_tag="PC472_000566"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2725928.1"
                     /translation="MQEKLLALREAALSEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(73211..73445)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        22827 a        13775 c         8056 g        28908 t
ORIGIN      
        1 tattatatta tacatatact tatttttact ataaaagtta aatttataag cttacttgtt
       61 ataaaaataa cttttataat ttatttcaaa aaaaatattg atttttgtgt cgcattttgt
      121 tataataaat taaggttatc cccctggtaa ccctagttag ttaatatatc tattcccaat
      181 accctttcat aaggatatat tttatattta ttaaaaatcg gattagtaac ggaaattaat
      241 tcggtggaaa aaaagagctt accccgagct cttttttttt ctgcaaaaaa tcgtaaaaat
      301 aaagttaata ttaatataac tttaatttta aaattaatat taaccatcac taaaaaagga
      361 tgtattgaat ttctaatatc aataacatcc tttttatata attatagcac ttttgctaaa
      421 aatgcttttg ttctttcatg ttttggattt ccgaaaactt cttctggagt attatcttca
      481 agtatacttc ctccatctat aaaaataact ctatctgcaa cttccttagc aaatcccatc
      541 tcatgagtta caattgccat tgtcatacct tctttagcaa gttcttttat aacatccaaa
      601 acttcgttta ccatttctgg gtcaagagca gatgttggct catcaaacaa catcatatct
      661 ggttccatag caagtgctct agctattgct attctttgct tttgtcctcc agataattgt
      721 gatggatatg aatctttttt atctaaaaga ccaactctac tcaacaaaat ttctgctttt
      781 ttttctgctt cttccttagt catctttttt aatttaattg gtgctaatgt tatattatct
      841 aaaatagtct tatgtgggaa taaattaaag ttctgaaaca ccatacctat attttgtcta
      901 acctcatcaa tattagtttt tttatcgacc aaattttttc cttcaaatat tatttctcca
      961 cctgtaggta tttctagtaa attcatacat ctaagaagtg tacttttacc agaaccactt
     1021 ggacctacta taaccattat ttcgccttta gcgatttcta agtctatatt ttttaataca
     1081 tttaaatctc caaaagattt acttaaattt tttattgtaa tcatattatc actttccttt
     1141 ttactattct atgttttccc taaatgctaa acatgctctc atctagttag acacagatag
     1201 tttcttttct aataatccaa ctaatcttga tagagtaaat gttataacaa agtaaattgc
     1261 tgctgctact attaatggct ctaatgctct aaatgctgca ttttttacta tatcagcagt
     1321 acgcattatc tcaacaactc ctattacaga tattattgaa gattctttta caagtgtaat
     1381 aaactcattt gctaatgaag gtaatatatt ttttatagct tgaggtatta ttatatatct
     1441 catagttgac caataattta atcctaatga tctacttgct tccatttgtc cattatcaac
     1501 tgattgtata cctgatctaa gtatctctgc cacgtatgca ccagagttta ctgataatgc
     1561 aaatgcacta gctataaatt cacttggtat accaaacaac atagggaatc ttattcctaa
     1621 ttgaggtaaa ccaaaccata ctatatatat ctgtactaat aatggtgtcc ctcttaaaac
     1681 ctcaatatag attgatgata ttgctcttaa tactttgctc tttgatattt ttgccataca
     1741 gataattata cctattataa atccaaagca taatgccaca agagaaattg atactgtcac
     1801 acctgttcct tccaaaaaag aagtcccaaa tctacttaaa aaactaaaat ctaaactcat
     1861 aatttcatct cccacataac tttcttacta atttgaagca gcttcagtag atgcttcatt
     1921 taaaaattct tctatcttct tgccatcttg taattctttt atctttttgt ttaataattc
     1981 tataaaatct ttgttattgt cagatttttt gattgctgct gccatacctt ctgttacatc
     2041 tttcccaact tcagtatttg ccatttttat tccatcatat tttttaacat ttattaaaga
     2101 aactgcttca ttagtaacaa ctgcatctat gtttccattt tttaattctg taattaaatc
     2161 aggtattgct ttcaaagatt ttgtagttgt catttttaaa gtgttaacta catattcttc
     2221 ttggatactt cctgcttgaa ctccaactct aagtttcttt aaatcatctt cagtttttac
     2281 tttgtctata tctgcatctt taactataac tgcatttcta cttaaataat ataattctga
     2341 aaaatcaaca ctcttttctc tctctggtgt tggagacatg cctgcaagaa caatatcaac
     2401 ttggtctgca tttaaagcac ctattaatcc atcaaatgac atatccttaa tttcaacctt
     2461 tacaccaagc tcttttgcaa attcttcagc caacattata tcgaatcctt ttattgaatc
     2521 ttttccatct actaccttat gaaattcaaa tggaggaaat tctgcactag taccaacaac
     2581 taattttcca ttgtctttta tttgttctaa ctttgttttt tctcctccac cactacatcc
     2641 tactagcgat aatgtcaatc ctaatactaa tcctaaactt aataattttt taaatacttt
     2701 cattttaact acccctctct tttaattata atttccctca gattttaata cttaaatgtt
     2761 tcgagttaca aaaaaagtct ctcagtcttt aatgactaag agacgacttt accgcgttac
     2821 cactcttatt gataaatact tatattactt tcaaaatttg tgactgttag cttttaagct
     2881 gtatggatat ttttatttta aaatcaaaaa agtcccttag ctttacagct aagagacgaa
     2941 ttatatccgc gttaccactc tttttgataa gtaagtactt atcctctcaa tcccttaagg
     3001 cgggtaaacc aattatcata ctaaagtttc tgataatctc ctccagagct ctcttcacat
     3061 agacttcatt gttaggctta caccaacccc taactcgctg taaatccata tatgctactt
     3121 tctctatcat tggatttgtt tccttaaatt gttaatacaa ttgtacttcc ttataatatt
     3181 tttgtcaata agttttttat ttttttaata atttttactt gtaaattatc taaatactac
     3241 tgtttatatt tattgatatt atttgtgtaa ttataaatag tatatttcat atttttagct
     3301 atttcttaaa taatactaaa taatatcttt cacattgttg ctttttctta aacaatacta
     3361 aataatatct ttcacattgt tgcttttctt aaattacact aaataatatc tttcacactg
     3421 ttgctttttc tttaataata ctaaatagta tctttcacac tgttgccctt tcttaaacaa
     3481 tactaaataa tatctttcgc attgttgctt ttcttaaatt acactaaata atatctttca
     3541 cactgttgct ttttctttaa taatactaaa tagtatcttt cacactgttg ctttttctta
     3601 aattacacta aataatatct ttcacattgt tgcttttctt taataatact aaataatatt
     3661 ttccacattg ttgctttttc taaacaacta taaataatat attaaaccct tatttctttt
     3721 ttctaataag ttaaataata cattttacac atatctattt gtcttgcaca taataaataa
     3781 tacattatat ttctatctaa atttagattc taaattaaat agtataaatt atattcttca
     3841 ataaacaagt tttcaaatcc aagattcgct ctctatcttt aaactttatt gtaaacataa
     3901 ttccatcttt tttaattatt tctccttcat gaaatttttt atggtatacc ttatccccta
     3961 caccaatact gtcaatttct tttttcgttg gagattttat atcattaata aataatgatt
     4021 tatcaacttt cttaccatac ttatttaaag tagagcttat acataacttt tcttcagctc
     4081 ttgtaatagc aacatacatc agtcttcttt cttcttctaa ttgttcttct tttttctctt
     4141 ccttacaaat atcatatgat ttttcatgag gtattgttcc ttcatttaca cctataatat
     4201 aaacatttct aaactctaat ccttttgcac tatgcatagt tgtaaatatg acaccctcag
     4261 tctgcttatt cttgttattt tccgttaact cagtctttac tctatcaata tgctctaaaa
     4321 actctgtaac agtattaaaa ttagttgctg aactctcaag ttcatttaat atctctacaa
     4381 gtccattagt ctttatcttt ctgttgctac aataatctaa aacatatcta tcataatcaa
     4441 gactagttct aatataagat attgcatttt taggatttaa agtatttaaa taacttaaat
     4501 ctatttccaa atcatttatt gttttaactt gtttaggatg aagattacac ttatttatta
     4561 atgctgttat aaagtctttt tcatccttaa ccatatttat actgtctttg gatatatatc
     4621 taaaaggctt gtttataatt ctcaaccaat ctttatttga ttttggatta atacctattc
     4681 ttaaataggc tagtatatct tgacttgccc aatggtcata tattgtaatt actgagtctt
     4741 ttactacaaa tggaatcctc atatccataa atacatctac taatgctctt gactgtatat
     4801 tagttctata aattactgca aaatctgaat actctacata atctttttgt atttcgtcaa
     4861 ttatttccct ggcaatatat aaggcttctt cctctgaatc atgcggagaa atataggtaa
     4921 cacttcctcc atccccctga ctacacttaa tgattttttc atatctattt ttatttttgt
     4981 cgataagtct atttgcagta tgcactatct cacttttaga cctgtaattt atatctaata
     5041 ttattttttt agtattatta aagtattgtt caaattcaag caaaaaatct ggtctagcac
     5101 ctctaaatcc atatatactt tggtcttcat ctccaactgc aaatatattg tttaatgggc
     5161 tacaaattaa ctttaaaact tcaaactgta ctttatttat atcttgaaat tcatctatca
     5221 atatatattt atacacattt ctaaccattt ctagtgcaga cttattgttc aaaagtaaat
     5281 aataagttct tataagcata tcatcaaaat ctattttatt aactttagat ttttgttctt
     5341 cgtataagtt gtatactttt aaaaactcat cttttgttaa tacttctgaa ttaaattcat
     5401 ttttatccat aagttcattt tttacatagg aaatttcatt tataacttgt cctatattct
     5461 catcatcatc agcattctct atatttaaac tttttaggat tgccttgatt gtcattcgct
     5521 ttgacttttc atcaaatata ctgtctaagt tgtatctttc aaagtatctt agaattttaa
     5581 aaaatacaga gtgaaaagta ccataggtta ccttattaag tcttatatca tcacttaaat
     5641 tcaaagctct atttttcatt tctattgaag atgccttggt aaaacttatt gccaagattc
     5701 ttgttggtgc tatgtttttg tttacaacca tattggctat tctatatgta attactctgg
     5761 tcttacctga accaggccct gcaagtatca tgcaaggccc atctatatgc tcaactgctt
     5821 ctaattgatt ttcatttaac tttcttatat ctatatttgt tgtgattatt agctccacca
     5881 ccttgattta taattatatt attttctttt taattataaa ctaaaagttc ataaaaataa
     5941 atttaatttt tcctctatta aaccacatca ttcctatttt catgacatat cttgagttct
     6001 tttttaattt tgtcgcttat atcttcaggt ataaatgatg gtttactaat atagtagcct
     6061 tgcatataat ctactccaag attaattagt gtttccattt caccttttgt ttctacacct
     6121 tctgctatca ctttaatatt tctttcgtta gaatatgaga taagattttt taatattttc
     6181 tgtctgtttt catctttatc aatacctctt acaatagaca tatcaatctt tacatagtct
     6241 ggcattatat ataatagtac agcatctccg ttgtaacctg tcccaaagtc atctaaagca
     6301 atttctatat tccaatggtc tatatatgct ttctttttct caataaaatc ttcattaatc
     6361 ttatcattct ctgttatttc tagtactact ctatttaaat attcaccata taaatcttca
     6421 atttcctcaa aatctacttt tgacaaatta ttattaggaa tagaatttaa gaacagtttg
     6481 caatcgccaa atttttcttt gtatttttta aatgatttaa gtgctttaaa ccatgtaata
     6541 cgctcaattt gatataattt agactgtgaa cgagctaatc taatgacatc tattggtgaa
     6601 ctaaaagttt ttattttagg tctcataagt gcttcatatg caaaaacttt acctttacaa
     6661 tctacaatag gctgaaatgc atactcaact aattgaccat caattaactt gtttaattct
     6721 tctctattac tcaataagaa tgaatcctta ttatatatat taatatcaaa ttcactaact
     6781 tcacccttga ttgtattttt aatttgatac atagcaaaat ctgcgtatct cattaattca
     6841 tcatagttgg ttgaatcatc agggtaccag gaaattcctg ctgatgccct tattttaaat
     6901 tctgttccat catgaagttt taataaggta ttattcatct gtttcttaac agaattaata
     6961 atatctctta tactatcttt actttcatat ccatatataa atacataaaa ctcatcacct
     7021 gaaagtctag atactatacc attccatgat ataaacttct taagtatgtc tgcagcacac
     7081 ctaatatatt catcaccagc atcatgtcca tatgtatcat taatatattt tagattatct
     7141 aaatcccaca taataaatgc tgctatctta agttcatctt tattcttaaa tttttcttgt
     7201 atctgtgaat gaaaagctct cctatttaaa agattcgtta aaatatcaaa atctctttca
     7261 tattctatct ttcttttctc aataaattct tttgttacat caacaacaac accaagaacc
     7321 tttagctcat cttcaaagat attcattctt atccacttaa gttttttatc tttagattca
     7381 aacttatata tatatgtatt ttctgtttca taactttctt gcaaatattt atctagtccc
     7441 cttaattttt tcaggaactt atttttttcc atatatccat attcttcaaa tttgatttca
     7501 gtagtaaaca cgctaaaaaa ttcttttgta caaaaaacat tttctgcgtt tttgtataac
     7561 tcaaaagccc caattggcat atctaccatt tcaataatct gtgataattt tgacgaagaa
     7621 tctgcaacat ttgcactgag caactctata gctgaagata attcatctat ttcagatata
     7681 tttatcttat caagatttac tggttttgat ggattactgt ttctaacttt gttggctaaa
     7741 agaacaattg gttttgtaaa taatcttgat gcaatgtaaa tacctattag acctattaat
     7801 aaagaaattg ctatagatat tataactaat ttctctaccc tttttgaaaa tgacaaaaga
     7861 cttttttctt caataatacc caaaagagcc caacgttcat tttcaaatgg agtattggag
     7921 ttatataaat ctaagtattg tacactagca taaatgttgc cattaccaca ttcattatct
     7981 ttatttttaa tcttatatat atttttatac gattccttct gctctaaatt agaatgtaaa
     8041 ccatcccaaa atttttcttt taatgagtat ccatttgaaa ttacattttc aaaggccata
     8101 tcattatttc tatctacacc taaaatataa ttaccctttt tattcccatt tatctctgaa
     8161 tatggaagta attctcttaa ataatttaca cttaaatcta ttccaagtac tccatataca
     8221 gttccatctt cataaataag gggaactgta tatgtcataa gcctatcacc tgcatttgtc
     8281 aataaaaact tacttcccca gtatcctaaa ttagaacttg atacatttgg attatcaatt
     8341 gcagctctaa atggtttata aaaaaaatca tctccatctt gctcattttc acttttaaat
     8401 gaaaacttag gttcccagta actgtccata gatattccaa aacttcttgc tatgtctgat
     8461 gaacctctct caattaacaa atcagaatta tcatttggat taaacttagg gtctaaatcc
     8521 cttatataca atcctgtcct acttatttca cctttactat ctattgactt atcgttaata
     8581 tttttgtcac ttaggattat aaaagctcct gtaacagaat tttttctcaa aagatatatt
     8641 aaatcatttg atatgctttt tataatatct ttgttaattt cttcattgtt gcctacatcc
     8701 tcaattaagg catttttttc atttaatact ttttttacag aggaattaat tgtagcttcc
     8761 gtttcacttg tatttgacca tctttgtaac atttcatttt gtatataatt tttacgatta
     8821 attactcttt catttaagat gtcaaacgaa ttatcattta atttcttaat agttcctccc
     8881 cataaaattg tggcacaaaa taaacttgtt tgaattagca taacaataat taggggaaat
     8941 atcatctttt tcattattga attgtttttt cttagattgt tttccatacc cattccacct
     9001 atacctttgt gctcgtcaac acactaactg ccaattcatc tattttttta tagcattttc
     9061 aagctcagcc ttaaaacttt caaaccaagt tttaaaattt ttatcattat taaattgtgc
     9121 tactgcatcc ttcttagaag ttccactttt tactaattca tttattttat ttgaatcttc
     9181 tttagcttta tcaagcattg aatattcaag tatttgtcta gctttagttc caccatcaaa
     9241 tgctttatta gtatataaat tacaactgtt aatctgttca atagatgagg ctattgaacc
     9301 ttcaacttta tcagataaac tcaaattatc tttcttcata acttctttta taacatccaa
     9361 atcattcgtt tttttcttta ctggtaagta tccagaagca atagagaatt ttatattagg
     9421 ttctttatct gtaaaccatt ttaaaaattc tactgatgca tattcttctt ctttagtaga
     9481 ttttgttaca gccattcctg caccttgttg tacagcatat tttccttctc ctttaaaacg
     9541 tggtgcttga aaaacagatg tttcaatatt ataactctca ttatcattta gcattactct
     9601 atctggaaag tatgctgctc ctgaactaga acctacaaga gctaatatat cacctgtttt
     9661 tgcatcatca gaacgaaact taccataaga agcaaaatat ccatttatat atggtacata
     9721 gaaattatcc catagttttt tcataacttt ttcatcaata tttagtgtaa ctttaccatc
     9781 tttaacagaa aaaatttcta ctcctagttg tttacttcct ataataatat aatttgctaa
     9841 agcatctctt ccaaaaaatg ctttaccatc attaggctct ttcgttaaac tatctgtcca
     9901 ttcataatat ttttttgatg tctctactaa tccttcaatt gtttttatat catttatatt
     9961 tgaatttgta gcttttgcaa atttatccca atcagtttta tttaacatca aaatttcagt
    10021 tgattttgca attgggaaaa gtttaaactc atccttagaa tttattctgc cttcttctac
    10081 atattcgtct atgtattcat ttaattcttc tttacttatg tacttatcca tatctactag
    10141 taaccctaat ttatccattt catatgcagt atctgagtat gctgcaaata tatttggtac
    10201 atcttcagaa ccaacttttt tatttgcaga atctaaaact ttctctgata aatcaactac
    10261 attaccttga ctgaatgctt caacaataat tcctttttca gcacctacag tgtcattaaa
    10321 ttccttaaca agcttatcaa aagccatttt ctgctgtcca ttataataat gccaaatttg
    10381 tattgatgtc ggattttctg ggtctaactt ataattatct tccttcttta aactacatcc
    10441 tgtaagtaat aaaacaatca ttgatgtgat aattaatttt gcaaacttac acctattcat
    10501 aaaatctact ccttcaaatt ataaaattta taatagcatt aaaactgtaa cttcactatt
    10561 tatattaatt atagaacgtt tattgatata tttaaacagg atttacaaca tatttaaaag
    10621 caattaagaa acatactatt acaaatatag aaaataaagc ttttacataa tttttatttc
    10681 taaatgcacc aaatgacacc aatccttgtt gaatagctac tgtaattact aaaagtatca
    10741 ttattatatt aagttgaacc agtttaaata agtaaagtaa tgaaaacact actaaaagta
    10801 cacataatac aatactagat ttattaataa tatcatcaat cttatctacc ttcttagatt
    10861 gcttattaac tttatttaat ttttctttat ttaaattctc aactttttta ttataccttt
    10921 ctcttgtttt atctttatat ttagtatttt tatttcccat ttattttcca tcctctataa
    10981 attttttaat tgagcttata cattatttaa atattatata tttaagatac catatattcc
    11041 tacaaaaagg taacttacta tgtatctatt tttgattttc gcataaaaaa gccatctcct
    11101 ttagaattaa ttctgtattg agacagctaa tttaactatt tatcctttga aacattttct
    11161 acattatcat atttactatc aactaaaact atatcaactc ttctattttt tgacctacct
    11221 tcttcagatg aattttctgc tattggtcta aactcaccat atcctacagc agataattta
    11281 tctggtgcta tacctgcatt gtcaataaga agttgaacaa cattggttgc tctcataact
    11341 gataaatccc aatttgactt aaactctgaa ttttttatag acatattgtc tgtatggcct
    11401 tctactctca tgtaactatt catttcattt aacatcttac ctatttgaat aatcttatct
    11461 ctagaattat ccttcacaat cgctttccct gtatcaaata gtattgtatc tttaaggctt
    11521 attactaatc ctctatcttg aactcttaat gatactgaat tagccatacc attttcgttt
    11581 aaatacttct ggatttcttc accaattttt ttaaacttag cattttgtaa agtatctaaa
    11641 tcattacttc ctgcttcaac tactggttct atcttgctat taccaccttc aataactcca
    11701 gaagaaccac caaaagcaga atttagagat tgagataatt gtttatattt ctccgcatca
    11761 acattactca tagaatacat tattacaaag aaaatcataa gtaatgttat gaggtctgaa
    11821 taagttaaaa gccatcgctc attgttttct tctttttcat cttcatcata caacataaac
    11881 attccatcct tttatattat tcagctctac caccaatttc ttcaagttca attagttctt
    11941 ttgaatttaa aaatattttt aatttttctg tcattaaatt agaattatca ccctcaagta
    12001 tggataatat tgcttctatc accaaaagtc tttcacttac ttcttgttga tttatagcct
    12061 taagttttga tgctattggt aaccatatta aattggcaga accaacacca tacaaagtag
    12121 ccaaaaatgc aacagaaatt tgaggtccta atgcatctgg attacttgaa agattaccta
    12181 aaatatgtac taatcccata actgtaccta ttatccccag tgtaggtgca tatccacctg
    12241 ctgaatcaaa aattgctatt ccttctttat gtcgttttga tgttatctgt atctgtgatt
    12301 ccaatatatt tcttatagta actgcatcca ctccatctac cataagttca agcccttttc
    12361 taataaaagg gtctaattca tcattctcac ttatttcttt gtcaatacta agaagcccct
    12421 ctttcctagc ctttgttgca atttctttga aatagattat attttctatg ttattaggct
    12481 ttttattttt aaaagctaat aacattatct ttatagactt tttaaacaca gggaatggag
    12541 tcgaagctcc aacagctcct atagttccac caaacacaac aagtgctgat gaaaatacaa
    12601 ataatgctcc aacatgtccc ccatcaataa caaaagcaac taccaaagat gctatcccca
    12661 ctatgaaaaa aattaaagtt gtcatctatt ccctcctttc tttagttata catatctaat
    12721 catttatgac attataaaaa gactatcttt aataaaaata gcctttaata attggcggag
    12781 aaggagggat tcgaaccctc gcaccggtta cccagcctaa tcccttagca ggggatcctc
    12841 ttgagccact tgagtacttc tccatatctc tatactagta aattatataa taaaatcata
    12901 ttttagtcaa ttgttttata aaatttgagt tattttgaat tattaaacat ctatttttac
    12961 ttttttgctt aaatgtaagt tttaaatatg gtaaaattat cttgcataac acttataata
    13021 ttatttgata aatatacata cattaacaaa tatacctaaa ctctgactta attgtaagtt
    13081 atgccaaaac taaaataata ctagtccaag gaggatattt tatgttacca caggaaagat
    13141 atgagaagat tttaagtatt ctcaaatcag ataatataat taaaataaat gacatagttc
    13201 aaatgtttga tatatcaata gaaactgcac gaagagattt agcccatatg gagagtatcg
    13261 gtcttttaaa aagagtttat ggtggtgcta taccagttat agcaagtagc gtagaactta
    13321 actatgttaa aagaaaaaaa ataaactcaa acgacaaaaa acagattgct attaaatgta
    13381 gtgagtttat aaatacaaat gatactattt ttatggatac tggaactact gtacttgaag
    13441 tttcaaaaaa tttgaaagat aaaaagaatc taactgtaat aaccaactca attttagttg
    13501 caaacgaact tatgaatact aatataaaag tatatgtgct tgggggctat cttagagaaa
    13561 ctgaaggttc tttttctggt ccgcttacac tgtcagctat tgaacaattt aatgttgata
    13621 aagctatttt aggcgctgga ggtatcactc ttgaaaatgg aatctctgac tatcacttag
    13681 aagaagcact tgttagaaaa aaaatgattg aaaggtctag aaaatctata gtagttgtcg
    13741 aacctcctaa gtttggtata aattcatttg catcagtagc tcctatagaa catatagata
    13801 ctattattac ctctgctagt atagaccata atataataat agagttttct caaaaaggaa
    13861 tacctttagt catagctgat aattagctta tatgattgat ttttagttcc ttgatgtaat
    13921 aaattaaaat taaggagcca aataattatt tatttagctc catttatgtc taaactatac
    13981 tttatatttt aattatgtaa ctactttcag ttagcttatc atttttagtt atttgattgc
    14041 cattttattt aatttataag ttttagttat tcgattattt tttaattgtc tgtcttttta
    14101 atttattttt agttatttaa actcctattt taaaataatt taaatttgac ttaagtattg
    14161 tttattctac actgcatctt tcttcatttt aatactttgt tcttcttcca aggttaaatc
    14221 ttttgtcata agtatagcaa gtgcaattgc caatactcct ccaaccaatt ttccaattac
    14281 aacaggtgta atcatctctg gaactactcc tgctgtaaat cctaaatggt cccccaaagc
    14341 tgctgtagca caaaccagcc aagcagtatt tactactttc ccctttggac tcatatcttt
    14401 catcatttta tatactggaa tactattggc taaagtaaaa actaatcctg ctgcactagt
    14461 ggcatccata cctaattttc cacccactat tgtaagtggt ttattaagaa ctttaactaa
    14521 taaatgtaat attggaaaag tacctaataa cacgattgca atacttccaa ttacttgaat
    14581 accttctctt ataggtgtca tgccttttat aagtactata cctgttattt cttgaaatgc
    14641 tgctgctccc aaacctatcg ttatcaatat agatataaat tttccaaaaa ctaatgaccc
    14701 atttatcata agcgtagaat taaattttaa acctactgcc aatattactg ccaatattat
    14761 cactggtatc atatttataa ttaccattga taagttaaat cctgctatta atcctcctat
    14821 aatacctcca aaaggtattg ttatcaagcc tatcagtaac ccttttgcaa agaatggtct
    14881 gtcttcatat tctataagac ctagtcctac tggaattgaa aatactaatg tacatccaag
    14941 catggatgac acaaccaatc ctgcaagcat tcctgcctct ttattgtgtg caagttccat
    15001 agccaatgga taccctccca tatcattcgc taaaatagtt gcaaacatag atgggtcagc
    15061 tccacagaga ttgaagattg gtattattat aggacctagt acatctgaaa ttactggagc
    15121 taaacaaact ataccaacca ttcctaaagc caatggaccc attgaattaa acccttcttc
    15181 aaactgctca ccaagtccaa atttatttcc aattattcta tctaacgctc ctaaaagcac
    15241 tcccacagcc attatgtata atataatttt atcaaagggc atttttaatt cctcctatac
    15301 atgtaaaata ctctattgta acttaattag cacacacctt tttggtatcg ttttcccaat
    15361 tcatagctct ttttactgct tggttccatc caagtaatat ttcttctctt ttttgagtag
    15421 tcatatttgg aatatatttt ttgcttattg taaattcact tctaatttca tcaatactgt
    15481 tccagacacc acaagaaaga cctgccatat atcctactcc aagtactgtc gtctcaggat
    15541 tttctgcaat ttcaattggt atacctaata tgtcggattg aaattgcatt gtaaagttat
    15601 ttttagtaat tccaccatct actctcaaca tttttatttt tttccctgag gctaagttca
    15661 tagaatcaac aacatctttg acttgaagtg caacagactc aagagtcgct cttactatat
    15721 gctctttttt agtacttcct gttatcccaa ccattgtacc tctagcatat tggtcccaat
    15781 aaggtgctgc tatacctgca aatgctggta caaaatatac acctccagta cttgatatgg
    15841 aatttgccat gtcatcagtt tctgaataat tttttataac tccaatgcca tctctaagcc
    15901 attgtattgc agcacctgct atatacactc caccatcata agcatatgta accttatctc
    15961 ctattttcca agcaacagtt gtcaaaatac cattacctgc tatatttagt tcctcaccta
    16021 tattcatata taggaaacat cctgtaccat aagtattttt aaccattcct ggttcaaaac
    16081 atccattgcc aaataaagct gctggttggt ctaccattga agcagtaatt tttatagaaa
    16141 ccccacaaaa tgcctcctta tctgtagttc caaaatcacc actcgtaggc attatatctg
    16201 ctaaaatact tctaggtact cctataatgt caagcaactc tctatcccaa tccatatctc
    16261 ttatattaaa aagcattgtt cttgctgctg tagatacatc agttaaaaaa gattttcctc
    16321 ctgtcatttt ccatattaac caactatcta atgttcctgc aagtacttct ccatttttaa
    16381 ttttttcttt tacacctttt acattatcta ttacccattt aatttttgta cctgagaaat
    16441 atgggtctat aattaaccca gtcttatcta ttatcttatt ttctaaacct ggtatattct
    16501 ttaaattttc cacataatca taagttctcc tgcactgcca cacaatagca ttgtaaagtg
    16561 gtttacctgt atttttatcc cataacatta ctgtttcacc ttgattatct ataccaattc
    16621 cttttatatc actttcattg attccatttt caaaagcctg ttgtaataca ttattactca
    16681 cagtttttgt attattccaa atttctatag ggtcatgttc aacatatcca gattgtggat
    16741 atatttgagt atgttccata taatcgctaa aaacaaactc tgatttttcg tttatcaata
    16801 ctgctcttgt tcctgttgtt ccttggtcaa ttgctaatac atatttcact ttaaatgtcc
    16861 ccctttattt tcagaattaa taaattcttt tttatttttt gaatatataa tctattcaag
    16921 ttatttatat tttatatcat catgtggatt tatgcaatat tcattacaat attttttgtt
    16981 atttttaatt atagtgatta aaaaagtatt tttattgaaa tgttattgca atatttatta
    17041 ttttattcta taatctagtt aaaataacac ataacatttt aatatttaca cataatcagt
    17101 cattcaaaaa tttaaattta actttttaag gaggctttac aatgaaaaaa acatatctat
    17161 tcagtcctgg accagtaatg gttacagata aagtcagaaa ttcacttctt cactatgata
    17221 tatgccatag aggaaatgag tttatggatt tattcaaaga tacacaacaa aaaatcaata
    17281 aactatataa tgcaacttct aattactact cattaattgt atctggctct ggaacatctg
    17341 taaatgaatg tgtactatct tctatctttg acaaagaaga tgcagttctg ctagttagta
    17401 atggtgtttt tggagaaagg ctagaagaaa tattatctac ttataatgta aaaacatata
    17461 aacctaattt tgaatgggca gaatacccgg acctagacat tttagaaaaa tacataattg
    17521 aaaattctga tataaaagta attgctatgg tgttccatga aactagttca ggaatgataa
    17581 atccagtccc tgaactagga aaacttgcta agaagtataa caaaatattt tttgtagatg
    17641 caataagtgc ctcagcaggt gaatatattg atgtggaaga gtttaatata gatataataa
    17701 caggtgttgg tggcaaagct cttggagcat ttccaggttc tgcatacctt tgtgctaaag
    17761 agagtatttt acaaaatatt aaagaaaatc aatgtaagaa tgtgtatctt aatctttata
    17821 aacactataa agttgctaaa tcatcttctc aaacaccaaa tacacctaat gttactttaa
    17881 tatttgccct taatgaagct ttaactgagt ttttggaaga tgacagtaaa atagaaagat
    17941 acaaagaatg ttctgctatt ttaaggaatg gtatggaaga attgggtcta acatttttac
    18001 ttccagataa gtatatgtct aatacagtaa catctgtatt tctacctaaa gaaatcaatg
    18061 taaatgagtt tatattaggg ctagaaaggg aaagtggcta tgttgtctat ccaggaaaag
    18121 gcaaattcct agatgctaac atattccaag tagcaaatat gggagaaata tatccagaag
    18181 attgttataa attcttagat atcttaaaat caaaattgga gataaaaaaa gttatataac
    18241 agtttgtttg taaaacccta caatatatca gtataaatta aatagactga tttttttaaa
    18301 ttttcctata ataaattata ttttatattt ttgtataagg gtgaactata atatgtaaag
    18361 ttcaccccta tttcaaatct ttttccttca gtataatctt tttcttttaa tttattttat
    18421 atttttgaca taattattct tacatttaat tgttttttat ttttaacaca atcgtttttt
    18481 gttaaatcat tttatattct tgatacaatc attcttacat tctattattg tttatttttg
    18541 acgcaatcgt tttttgttga atcattttat attcttgata caatcattct tacacttaat
    18601 tattttttgt ttttgacaca atcgtttttt attcagtcat ttaatatttt caacacaatc
    18661 attcttacat tctattattg tttgtttttg atacaactgt ttttattcag tcatttaata
    18721 ttcttgacac aattattctt acattctatt attgtttatt tttgacgcaa tcgtttttta
    18781 ttcagtcatt taatactttc gacacaatca tttttacatt ctattattgt ttgttttgat
    18841 acaactgttt ttattcagtc attttatatt ttcgacataa ttattcttac attctattat
    18901 tgtttatttt tgacgcaatc gttttttatt cagtcatttt atattttcga cacaatcatt
    18961 cttacattct attattgttt gttttgatac aactgttttt attcagtcat ttaatattct
    19021 caacacaatt accttttcat tcaatcaaag taatctttaa gcattatttt tcaagctcat
    19081 caaagtaatt atatacgata acctcacctg tctctttatc tgtagtcttt ataatgccct
    19141 tagctccatt tacctcttct tttgtaaatt catcatctag cacaactttg aatttatctt
    19201 cctttccata accttgttga aattcatctc tcaaaattgc atttttttct gcttttggct
    19261 tcaactcaaa ttcaattcca gatttcattt catcaaaata atttaatact acaacctcat
    19321 tattcatttt attagttgtc tttataatcc ctttagctcc atttacttct tcttttgtaa
    19381 attcatcatt caatgttata ttaaatttgt cttcttttcc atattcctct tgaaaatccc
    19441 tcttcatttc attgtattta gtttcaaact ctttactctt gtttaattcc atagctgata
    19501 catttgtcaa tgatgctcca attgccatta ctgttaatgc cattactata ccacttttaa
    19561 atttttttac tttcatatct tacctcctaa attaaatgtt ctcaatgaaa ttatataaag
    19621 aaaatcctaa gttttacagt ttcattcttg taactaaaat tactattatg taaccaaact
    19681 ttttgcttca actattcctt aaaagttgct tttttgagga atagttgaag ttaaataatt
    19741 agattttatc tatatttata gatttaaaat ctaatttatc catataaata aatacttatt
    19801 tttgcaaaat aaaaattttt aattactcca atccttctta tttacttaaa atcctatgat
    19861 aagaaattta aaagtcttta ccttctttaa caataattta tttactttta acatacaaaa
    19921 tatagagagt atatcaaaat cattttactg aatattctaa aaataagtac cccctattag
    19981 tatatatatt tacatattaa aaaaaagttt taaataaagt tatatttttt acaacagttt
    20041 tgtaattaaa aacatattga aggtaaattt aaaggtacta tatactataa atatgtcgac
    20101 acaataaatt taaaaaggag aactaaatat gaaaaaaaga ttaataataa tgatattgtc
    20161 tgttgtctta gtactatcaa gtatacttac gatatttgca tattcaaata ttaagtataa
    20221 taataataaa ttgatatatt ctaatatgat agataaaaaa actcaaaata gtgtaaagga
    20281 aatcctaaaa gaaaataaaa ttaatgaaaa agatatagat acatttatta aagcagtcaa
    20341 caattataat aaattgcaag ttaaaatact tcaaaataat ataaatatat ctaaatctgg
    20401 atattcttca atcaatgcaa aacaagttcc atataaccta gaaaaattgc aagataattg
    20461 ggtgaaaaaa ttccctgatt atatggatgt aaattgtaga ataactgctt ttagactttt
    20521 taaagatttt ataaattcaa ataagaaatt tactggagac tctattgatt tgaatgtcga
    20581 cttggacact ataatgaata ataaagatgc taaatttagc accaaagatg tagaaaaatt
    20641 tatcaacttc ttttctgcaa taccagctaa agatacagat gatacaatca aaattgcaga
    20701 acaaataaaa aatgaatgga aaaaaagaaa aatatctttt aaaaataata aaaatatatc
    20761 tattataaat ggatttcttc gttatccaga aactaaaaat gtatttatag gtcatacagg
    20821 catatgtatt aaaactaaaa atggcattct atttttagaa aaatatggtg ttacatcacc
    20881 ttatcaagtt actaaattta aaaataaaga agatgtaaaa aattatatgt ttaatagatt
    20941 gaagatctca gaaggtgaaa ttgaattacc tgaccctata ataatggaaa acgataaact
    21001 tatgaaataa taactttaaa cgcaaaatag tatttataat tactaagtca atataataga
    21061 gaaggtctat agatttgtgt aaatacaagc ttatctacaa tcttatataa gaatgtttat
    21121 ataaataaga tagattaatt ctaagtccta ttattctatt gaattttatt attattttat
    21181 ttagattcta ttgttatttt acttagaact tattattatt ttgtttaagt tctattacta
    21241 ttttcaatca tataaattta caaaagactg tacataaatt tattttatac aaacttatct
    21301 acagtctttt gtgtgaatat atctaaataa tatcgtattt atttatttcc tctgcacagt
    21361 gagaatcaga acctaaaact aatggaatat cgtatttttt taccaagtct aataagtatc
    21421 catctatata aggctctttg cagtactctt ttctatttcc agaaacatta taatcaagtt
    21481 cataaccttt ttctttaatt aactttatta actcttcaag aattttattt cctttataat
    21541 cgtatggaaa tacctgatta aactttctaa ctaaatttaa atgacctatt ctttttggtt
    21601 tatgtatacc taaatctgaa tttacagctt ttatcaatgt ttcataatac ttattataaa
    21661 cagcttcaat acttcctaat ttgtcgatta aattttgaaa ctcctctaca ctgtaatcta
    21721 cacaataata atcatcatca atcttaatta tatgtactga taaaagagaa tcctctaatt
    21781 cttctccata tttatttaaa ttctccttta tcccttcttc gtatccttct ataaaatcca
    21841 cttccgaccc aacatttatc ttgattttac cctcatactt ttctttcaac ttcttgacat
    21901 cattaaaata ctttggtaaa tcttcaatac tcatacaact atcattttca ggagcagggt
    21961 ctctaaatcc atttgcatac ggaaaatgtt ctgtaaatgt catttctgtt attccaacat
    22021 ccattgctct ttgtacatat ttttcaaaat catccttact tccatgagga caataaggtg
    22081 aatgtatatg accatctttc ataaataatt cactcctttt ttaaagaaaa aatctaatat
    22141 aaatgcataa atcttttaac ttcaaaataa tatttttaat catgctaatt tttattatat
    22201 ctaatattag agtaatttta aactgccttt taagtatctt atcattagta ctattttcaa
    22261 cttaacatta atattatttt ttaacttaac attaatacta tctttaaata gtaatactca
    22321 atatataatt acttaacata aaataactca aattgcttga tgaggctttt gcagtattta
    22381 aagaatatat aattatttaa cattaaatta tccaatcttg taataaaatt tactcattaa
    22441 agtatattac agaaaatacc tcaaaataaa ctttttagtt catgagatac tcttttttac
    22501 atgaaattag atatattttt atcatttaaa caatgaaagg gagcttatat ccatctttag
    22561 acaacccctt atataaaatt tttaataatt aaacatcaca aaatataaaa atcgcttata
    22621 tcaaacgtat aagcgattaa ttatatttac atatgttttt tagcctctaa caacatttta
    22681 ttagcttctt ccatatactc tatagccttt tcaatactct ttgctgtttc ttcttttcca
    22741 atagctctag ccttttcaac ccactctcta aatccttctt catgagtttc attgtgattt
    22801 atccaatgta ctaaaagtat ttttaaagtt ttttcatcct tagactctga tgattcctca
    22861 tgactatgtt cgtggtcgtg gtcgtgagca tggtcatgtg gatgttcatg acatatatct
    22921 ccatgagtat gttcgtggca gtggtcatgg tcatgatgtc catgatgata tcttacaatc
    22981 tctttatcaa aaatcattag ttattccccc tgttatttta tattaaactc tttttttata
    23041 aggtttacag aatcttttac aagtagtgct aatggtttac cttctaatcc aactaactcc
    23101 acattttctg gcataatagg taaaagtatc ttaagtgcct cactgtctgc aatagcttca
    23161 cttatattac aagtaacttc tcccatcata gaattaggta ttattacaga aataggtgca
    23221 actattatat ttgctttttt agctgatact actatagcat tttcccctgt agctccttta
    23281 ttagcatgag ctttcatcat agaactagtt gctatagaat tagtccctag tgcataaatt
    23341 tctacatatg taggtaactc ttctctcaaa gaagaaacta tctgggctcc aatacctcca
    23401 cccattccat ctatcactgc tattatcata tgaaattccc ctattaatta attttcttgt
    23461 attattatct tatggtccat caatcttatt tctttaatta tcccatctat tatcttttgc
    23521 tctcctaata aatccgttaa ataaatcttt ccatcacaag ggtctatatt aaccacattt
    23581 tccataacct tttctaactc attattactt ttacaaatga aagctgatga ttcacacata
    23641 taaatctcct cctaatatga tttttattaa aaatcaaaaa aagattccct taggtatttc
    23701 aatacaccaa agaaaccttc atgatttcag tttttgtata ttattatacc ttaagtatat
    23761 cactttaatt tatgtaaagt caattaaagt taacgctttt tattatactc ttcattcttg
    23821 tatttttcat atagttcttt aactaattgc ttctcataat cagtcaaatc gccctccaca
    23881 aactcaacat taaattgttc agctaatccc tttactatgt ttttctgtag aatatctatg
    23941 tctatttttc tgccaatttc attttctatt ccacttgctt ttttagctgt aaatttcata
    24001 gctctttctt tcaattctgg tgtcttagtt tttattaatt taaacaattt ttctacatca
    24061 aaatccagta ttattgaacc atgttgtaat aaaacaccat cttttctgct ttgagcactt
    24121 ccaattacct tcttattatt aatagtaact tcatatgatg catgtgcatt aaaacatgca
    24181 gctgataaat tttccctact tattctctcg cctctattta aattatctgt ttctatacca
    24241 cttagattca atcctttaac tagaccttca cttatatatc tataagatag gttgatgctc
    24301 ttatcgatta aaggattatc ttctcctata attatactat aggttagctc attatcatgt
    24361 aatacagctc ttccacctgt aattcttcta gtatagtcta tattcataca tctacactta
    24421 tctaaatcta tctcatcttc caatttctga aaatatccta tactcaaaca agctggttcc
    24481 catgtataaa atctaagagt tggcttatta tgtcctttct tatatgcagt aaatatagct
    24541 tcgtcaattg ccatattcat tgctccttca tatgatttat tatgtataac tctccactga
    24601 ttcatgatta ttacgccccc atttttagta taaatatatc ctagaaatcc tctatagatt
    24661 tatttctctt attgctaaat ctatctcact ttgatttaaa ggtcttgaat agttgtatat
    24721 agtacttcct ggtctttcat tatagtatgg tctattacaa tccttacaac ctcttatttc
    24781 aaaaggatag cctttgttta tgtcttttaa aatatcattg tcaagcttta tactatttaa
    24841 gtatccattt ttaaacttaa aatactcttt tggataacct ttatttatca tataagacat
    24901 taactgtacc cttctataac tttctatgct tggttgactt atcttttcca ttttagttcc
    24961 tctaactgga gtaaatgcaa ataaactaat cattacatca ttttccttta aataagtata
    25021 taaattgtat atatcttcat gactttcacc cataccaact attatatgtg tacttatttt
    25081 attgggatat gattttgaca tttcagttat aaattttaac ttttcatcat agttatttcc
    25141 ttttattttt tcatatagtt ctttatttgc tgcatctatt gctattccta ttttatcaac
    25201 tcctactgaa aaaaacttct ttatctgttc atcattttct aattttgcag atagtgaaat
    25261 tggcatgtct atcttaccac ttatatagtt tataaagtct aaaacagagt tatgtgcttc
    25321 ttcacttgcc attgattgaa tacatattct ctttatattt tttcctttat aagcttttaa
    25381 agcatctaat atttcttctt ttgaaaactc tggccaaatt actcttgata atttatcttt
    25441 tctagtacta ctttctattg attgagaaca aaaactgcat ttatttatgc atttttctcc
    25501 aatcattata tatgctgtgg taggaggtat atcgcttttt ttgtttaata tcccaagttc
    25561 tattgctgtc ccaactgata gtctaatcat aaaacgcaac actccttaca ctcatttatt
    25621 gtcatattca tttctatagc cttattcttt gcagaccttg atggtaatac tattctattg
    25681 actccacaca ttatagataa ttggtctagc tcttttctat acactcctct tggtctcata
    25741 caacctagat taatttcagt atctggtaaa ttaattctag cttcacataa aatatctgct
    25801 accccttcta actcaggagg ttctacattt tcatactcag ttccttttgt tggaataagg
    25861 actataaatg taagcttatt aggaggattt ttctgtaggt attctataat ttcatattca
    25921 ccctttattt gtcctccttt taaaccaata catatatgtg gtgctacttc tgtatgttct
    25981 tgtattgtat tatagacttc aatataatct tctttactct ttttcatctt ataaacttct
    26041 cttattgtct catcatcaaa cactaaatca aaagaaacta tatctaaata cttacacaac
    26101 tctacaatgc tatctttatc cataagacct aaatgagcat tcaatctata tccttgctct
    26161 tttaagtttt taatggtatt aatatgagta tttataggga catctccctc ataactacaa
    26221 ccgccactca atagaaagct agttatattc cttgattgta ctttttcctc atattcttct
    26281 attggcacca tattgtttaa gtagtgaccg ttgcaatggg cacatttcaa actacattga
    26341 ttgcttgtag tactaagagc tagtgtctgg ttaggataag caaactctat tttatttcca
    26401 aaattcttta atttaatatc aaatgctatt ttcatttttt cagatagatt cttattcata
    26461 gtttattcct tcttaagtac attttactct tctagtaatt tctttgctgt tgggaactgc
    26521 tctatattcg tatgtggtaa gaaacatgca gaaataaatt catccatata acttggatat
    26581 acactaagtt caacataagt tatttgatta cctatttctt caagtttatt ttttgcatct
    26641 ttacttataa gagctaagta agaaccaaca agtgaactat tccctatata agtaaatttt
    26701 tctctatcaa tatcaggaag taaccctata attattgagt tttctatgtc taggttgttt
    26761 cctataccac ctgctatata aaccctatct aatatactaa aatccatacc taaactatct
    26821 acaagagtgt atgcgcctga atatatagct cctttagctc ttatgaaatt atctatatca
    26881 acttcattga ctactatatc attttctaaa tcaaattctt ctttaaatgc taatacatac
    26941 tctcctatct catgctcatt aaatctaact ctcttattat ctaaatccct gtatatttta
    27001 cctcttctat ctattacacc tttggtaatc atttgacaga ttaaatcaat tataccagaa
    27061 ccacatatac caactggtgc acattcatca ataatcttta atgttggctc taaagtatct
    27121 ttatcaataa ttactttctc tattgctcct gctgatgctc tcattccaca gcttatccct
    27181 ccaccttcaa aagcaggtcc tgctgaacat gcacagctca tcatataatc tttatttcca
    27241 aatacaatct ctccattagt tcctaaatct ataaataaaa cattttcttc acttgaccat
    27301 atccctgctg ataatactcc tgctgttata tctcctccta cataacttgc aacagatgga
    27361 gctaagtaca catatgcact gtcatttaca aaaagaccaa cattttcacc cataagtttt
    27421 ggagatttta aaaatggtgg tatgtaaggt tcttgtctta agaagtctgt atacacaccc
    27481 aagaataaac ttgtcatagt tgtattccca gcagccacta atgtaactac atcttcctta
    27541 tctattccat ttctttcata tattgttttt aatagtggat ttatagtttc ctcaactata
    27601 gctttatgaa gtgtctctaa tccatttttc ttagttgaat atataattct atttataaca
    27661 tctgctccat attttatctg tgcatttcca gaagatgctt tatctactac ctcttttgag
    27721 tataggtcta ctaaacaaac aactactgaa gttgtaccaa tatctattgc aactccgtat
    27781 aaggaatttt ctttatttcc ttgttctata tttatgattg taagtttctt ttgtttttga
    27841 acatatgtta ttgtaacctt aaaatcactc tttctaaata cagttggcat ttttctaagt
    27901 atatccaatc taaaatctat ttcattatat cctaggttat ttctaacata tctttccaat
    27961 ctatctacat cacttatatt atcatctaaa tttggttctt ccatttctat gtatttcttt
    28021 tttatatttg ttttaaattg aaggttatgt tcttcaatta tttttttagc tctttcaaaa
    28081 atttccctat cctctttttt attactacct tctatcttca taccatgcat agaagatgat
    28141 actttagaag gaacttcaat ttctatatct gatattacct ttgtacaaca agctagtata
    28201 taaccttgct cccactcatc atctgttata tgtcttgttt tttctgtatc aacttttcca
    28261 ttcaataatt ttactttaca cttcccacaa gacacgtttc cattacaagg agcatctatg
    28321 aatatatctg catttcttgc aacctctagt aatatatccc cctcattaca ataaacctct
    28381 ttattgtttg gtgtaaaact tacttttatc atgttatccc tcccaatttg ttctatatat
    28441 gataaaagga aagtatagct aaatttgcgt gcactcaaat tttactatac tttccccaat
    28501 tataataata tcttaatctt taattattct aatccagcct cgtattcttt atcacttaat
    28561 aacgtatcaa gttcagccac atcagctagt tttactttta atatccagtt ttcatatggg
    28621 tcttcattta tacattctgg ctcatcttct aatttttcat taacttccaa tacctcacca
    28681 ttaacaggag atattaaatc agaagctact ttagaagact caactactcc aaaatctact
    28741 cccttagtta cagtatcttc tacttctggt gtttcaacaa acaatatttc tcctaattga
    28801 tcttgagcat agtctgttat acctatatat acaacatctc catcaattac ttttacccac
    28861 tcatgatctt tagagtattt taattctggt aataatttca tttcgataga cccccaatat
    28921 tatattattg attaaattac ttaaatcata ttaatattat atcatattat tttaaatttt
    28981 ccatcaatta cattacactt tccatagcta atgctggatg acctttttct tctaagaaag
    29041 ctaatactcc ttcagaatcg tcagctatag tttcatcaca aaccatatca cagaagtttt
    29101 ctattccagt catttcttta actgtagcat ttaatttatc agctacataa tcttttaatt
    29161 cttttggcat ccaaactatt ctttctggtc caccttctgc ataagcgaat tttttagaag
    29221 atatgaaatg tcttccgtgt cccataaatc ctggagtttg aactcctcca cctgtcatag
    29281 aagcaagttc tccgaaagtc ataccaactg gagtaacact tgcaaattct ctgttaacta
    29341 caacaaatcc atttgcttct ggcattatac cacagataca ttcgaagcat ccacaagaag
    29401 tcattgggtc ttctagtata gagtataatg taacagattc aactgcacct tgagatattt
    29461 ggtttacagt ttcatttact gaattccata cacctgttct atcatctaag cactcgcctt
    29521 tttctattgg ttgacaagga cctgttgggt ctaattcttt agtagcctta gcatctaacc
    29581 agctaactgc tccacaaaga ccaagtcttt caggagtaac aacacaaacg tgagctggtg
    29641 caaatgattg acataagtta caagaataga aagtgtcaac actttcatca actaatgaag
    29701 ctaatctttc atctctagca ttgtacttag ctattgcttc tccttttaat tcagaaactt
    29761 tttcagcatc tgtaattata gttatttcac acttatcaac tactgattca aattcatcta
    29821 acatcttagc atataaaact tctcctatat gctcaagtct aaatccttta tcaaatgcat
    29881 ctttagagat tcttacccaa gccatatctc tttgtccaac gtgcattaca ccttctatat
    29941 agtttaagaa gtagtggaaa cgtctttcaa gaactggctc gaaatcttct tgcatatttt
    30001 taccagctat tttaactatt acagcaagtg gtaatcttaa tacaccatct gcatcaactt
    30061 catctatatt tggtcctatt atttctatct tatggtcttc aacttctgtt gcttctttct
    30121 taacaacaag ctcccaagct tcagttctgt ttccaccaaa ctcagcaaac atatcgctct
    30181 ttctaattct ctcaccttca aatgctgctg caaatgaaac tgggataggt atttcagtaa
    30241 cttttatttt tattcctcta gcttctaatg aagtagctac tatcttatcg taatcttttt
    30301 gagttaatag gttcattgga acttcaagaa ctgtttggtc agttataact gggaatccta
    30361 aagctattgc tccagcacct gcagatacaa ctaattcact taatggtcca aatgcattaa
    30421 caaatgcagg aactctatta gctgtgtact ctaataatcc gtttaaatca ccaggagtta
    30481 atccaccgaa tattaatgct gctcttactg caacagatac aacgtgtata acagaagtta
    30541 catcatatcc tagaggtata actcttagct caagacccat ttttactcca gcttctatag
    30601 cttggtctat aactttacca actaagaaag ttagtaaacc tttagattgg taatctttta
    30661 taacttttgc tgctgattct gcatctggac actcaccaag aacaactgca acaccaggta
    30721 tatctcctgt aactaatggt acaccaagtg atctgattat agggtcagtt atatgacctg
    30781 cacatggctc actatatgga gcatccatag ttgaatattt taatgcttca attacttctg
    30841 ctgctaaagc tgtagctaaa ccagcattaa aagcatgttc tagtaaatga gttctattta
    30901 ttaaagattt tactacttct aaagcacctt ctaagtcccc taaagtgttc atcttttgtc
    30961 ctgttgcagc atatatacaa ggtaatgaat atgctgtatc agggaaagca actttatgct
    31021 cttttccatt cttttctata gcttcagcta acatagcttg agccgcacct aaagcttgtt
    31081 ctgaccctgt aaagattata ttatatagat tcattctatt tcccccttta atatttacac
    31141 ctatttttta ttaactatac cctaaaaacc ctagaaagca aatccacctt catttaactc
    31201 taagaaagag tctgcatata aagatgcccc gttgattatg tcacaagatt ttacagtttt
    31261 cattaattct gggtcacatg gattcattat agctgaacta aatccattag ccattgccat
    31321 tgctaagaat gcactatcta aaacaggtct aacatgctta ggacatccat tagatacgtt
    31381 agataatcct cctgtagtta aaagtcccat ctcagtcatc atttttatag cttctaaaac
    31441 ttctacttgt ttttcttgca taccttttat aactaagcat aatgggtcaa atagtatatc
    31501 agttggctcc attcctaaca ttaagccttt ttctaatatt tcttggcagt aagccatacg
    31561 ctcatcatta tctcttggga taccttcttt tgcacataaa cctataacca ttgcttcgta
    31621 ttcagctgct atatctatta aatcaaatct tgagccagca tcagcagagt ttattattgg
    31681 ttttgcatta gttctatcat aaactttaag tccagcttcg atagcttttt tattagctgt
    31741 atctaatgct ataggaacat tgttgaactc agattgaagt aatttgattc cccatgtcat
    31801 tatttcttcg ccatctcttt cggcaggtcc tatattaaaa tctatatagt gtgctcctgc
    31861 ttctaattgc tcttttgctc tttttaagat tggagctggg tctctttctg ctaatgcttt
    31921 tcttattgaa ggtgatatac aatgtattct ctcacctata atcataaatt tttccattta
    31981 taatgtcccc ctttaaattt aatattaata gttagttaat attttattag tttgtttggc
    32041 atgctttttc ttggtatcct tttaagaact taggtaattc catagattcc tctggtccta
    32101 taactacatt ccatcctggt aagttatctt ctatatcacc tttaagtact gcaacttttc
    32161 ctggaatgat aaggtcttta caattagtta cttcttctac cttactttct ttaatgaaag
    32221 cagagattga gtttccacca aatttaccag cagcccaaga tgtaagaact gaatatccac
    32281 ctgcatctgg tattactaac catactggaa cttttgatct ttctatatca ccagcaacta
    32341 tgaagtaagt taatgcaaag tcaacagtaa ctaatactgg agaattttca tctggattgt
    32401 tgattggata aatctttggt tcaactctca ttggtctttg tgggtcagtg tatatgtttt
    32461 gtcttagtgc aaataatggt aatgccttag cataactaat atcatctatt actattatag
    32521 aaccgtattt tatagtgaat attgatgata aagcaacttc catcataggg tttgaattag
    32581 ataatctatt tgcaaatact attgaaggat atccaaatgt tctatcttgc tcttttaaag
    32641 ctgttcttct aacttgtata gcattagtgt aagtatcttt tatgttttct cctgttacat
    32701 ctaatactaa ttctttatat ccagcggctt gaattaattc aactgtttca tataattctt
    32761 ctaagctacc agcttttaca cctaatggta aacttgctcc tttaactact tctatcatat
    32821 ctttgtagtt ctctttagtt gctccatata ccattggtct agcatctttt aatacttcaa
    32881 cagcttcttt aactacttgt gcatcatcac aagctaaaat ataagctact tctaatccac
    32941 tgccttttat tttatctatt aattttaaat acgcatcttt atctccacaa tactctaaaa
    33001 cagccatttc aactttcatt tgttcaccaa ttcttacata gtcaactttt ttcatgttag
    33061 ctattttagc atctactgct tcatcactca tacaagtaca gaatgaaact gcatatctat
    33121 ttctacttac taatgttttt tcatgtctaa ataatactgt ttctccacct agttcatact
    33181 cagatgcacc agcaccaact tttaaagctt tcattaaagg tgcagtagcc tctgataatt
    33241 ttgctatagc gtcatcagac atatgtgggc atttacctac ttctacagct cctgaagcaa
    33301 ccttcataga gaaagccata caagttggga atccacaatc cttacagttt ttctttggtg
    33361 ttaatttaaa tatatctaaa gcttttaatg ccattatttt ttccccctaa ccttaacaat
    33421 ttaattaagc taatgcgtta accaattctt ttatagtttc tattgatttt ggatgacgaa
    33481 gtataactgc gtttgaacca cctactaaaa cacttgctgc agttgaaact tccattgcta
    33541 tagttctttc ttctggacaa ccccaatctg gctcatcttc tattggagct atagcttctt
    33601 taacatgacc tacttcaaaa gctactggtg ttatgatagg catttgtaat gtcttatcat
    33661 tttgaccaaa tgctgcaagt ctaattctat ccatagtaga tgcaacgtac tcatatccat
    33721 aaccaactgc tgaacatcca acattcataa ctatattttc acctttaacg cctagttgag
    33781 ttaacaatac gtttaattgt ttagctaggt ttatatcaac agaagattca gctcctactt
    33841 tatgtgcata agccatacca gctgatgctc ctactccttt gtagttatct tctactgctg
    33901 acataaataa gcagttatgt ccatccaatg tttgagctaa tttttcaaat aatttaccat
    33961 ctttttcatg atttcctgaa ccagctacaa ctaaaggtaa ttttattgct tcaattactg
    34021 ccttagctac atcagcacat tcatcaactg acttatctaa tccatttggg tcagaaccat
    34081 caaattttaa acaaataaaa tctgcttgtg tattagcttc aacatattta gcccactcta
    34141 ctggacaatt agctacatct ttgtataatt ccttatatga atcagtccag ctttcaggat
    34201 aaacgtctga tatttgtata cctatttttg gagaattacc tacttctcca tcaaaactat
    34261 aaaaaggtaa tacattttcc ccacctaatt taattgcttt ttcccctatt cctacttcaa
    34321 cttctgatat ttttccagaa tatttttgag tagacatttt aaatgccata tttttttccc
    34381 ccttaagcct attttaattc taatttagca aaaatttctt tcatagccac tttagatttt
    34441 gaatcttcag gtaaattaac taaaggaata cctgtagaat catattcata tatcaattca
    34501 tccataggta caacaccgat tagctctagg ttatgctttt caatttcttc tttaacacca
    34561 tcattcataa ccccatttgg aactttattt acaatcaaaa gtattcttcc aacacttaac
    34621 tttagttcct cagctaaatc tcttattcta gcaacagctt gtatgctacg tcttgagcaa
    34681 tcactaacca ataaaagtgt atcaacatgt ctagtgactt ttctacttag atgttccata
    34741 ccagcttcgt tgtccatgac taagtatttg taatgaccag atattttgtt tacttgttct
    34801 ctaagtatcc catttacaaa acagtaacat ccttcacctt ctgacctacc cattactaat
    34861 aagtcatatc cttcgccttc ttcgattata gaatttaatc taaattgaag atattgtgcc
    34921 tttgtcatac caccagggaa tgcattgcct aacttttctc tttgatttac ttcttctctt
    34981 atttctccta ttgttgcttc aacttcgata cctaacactt catttatgtt agcattggca
    35041 tcagcatcta caactaatac tggtcccttt ttgtccttaa ccaaataatc aatcaaaagc
    35101 cctgtgagac ttgttttacc agttccacct tttcctgcaa cagctatatt gtatcccatt
    35161 ttaagacttc ccccttaaat ctagccacag agccttatgt actctgtctg tctattataa
    35221 tttaggtaca gaatgaacac attctccatg tacatcatga agtgcttcca ttagcccttc
    35281 agataaactt ggatgtggat gtattgcatc ccctacttgt tctactgtta atcctagatg
    35341 aacagcaagt gacaactctg tcaataaatc tgtagcatga ggtcctacta ctgctgcacc
    35401 tattatctta tcagtttctt tgtcagctat aacttttaca aatccttgga aatgtcctat
    35461 agcttgtgct tttccaagac ctctaaaatc aaattgacct acattgtatt ctacaccttc
    35521 agcttcaagt tgcttttcag tcttaccaac accagcaact tcaggttcag tataaacaca
    35581 tcttggaatt gctctataat ctactacctt agtcttacct aaagcatttt caactgctac
    35641 gataccttct ttagaagcaa catgtgctag gaaaggagta tctattatat cgcctattgc
    35701 gtatatacct tctacattag tttcaagatg ttcattaact actacttttc ctctttccat
    35761 ttcaattcct atatcttcta ctccagaatt gtcaaggtta ggtcttctac caacacatac
    35821 taatgcatat tgtgcttcta taacctttcc attagaaaga gttgcaacag ccttaccatc
    35881 aactacttca caagtttgta ctccaatacc tgtaataacc ttaattttat ctttcttaaa
    35941 ttgtctaagt agttgtttag ctacatcttt gtcttcattt aatagtattt ggtcaaccat
    36001 ctcaactata gttacttctg ttcctaaggc tctaaagaac tgccctatct cacatcctat
    36061 aactccacca cctactatta acatagactc aggtatctct tcaagtccaa gaacttcatc
    36121 actagttatt acaacttttc catcatatgg gaacattctt ggtacaactg gaacagaacc
    36181 atttgctagt attatcttat ctgcttttat agtttctact gttccatcat ctttagttac
    36241 ttctatagtg tttttatcaa ttaattttcc aaaaccatta actaagttta ctcctctttt
    36301 ttcaaataag aactctatac cacttattaa ttggtttaca actttatttt ttctttccat
    36361 tatggcagtg aagtttggct taattgtacc atctatttct ataccaaaat cctttgcttc
    36421 ttttacagta ttcaatactc ctgaagaagc aagaagtgct ttagttggta tacatcctgc
    36481 atttaagcaa gttcctccaa ctcttctttt ttcaacaact gttacatctg caccaagcat
    36541 agaagctttt atagctgcta catatcctcc tggtccacca cctactacta ctatcttcat
    36601 ctttaattcc cccaaaccta atataatcta caatcttgca atactataaa cctgcggcat
    36661 ccagtatagc aggtatattc tcttctgccc caattgccat tatatgaaca ccatcacata
    36721 aatcttcttc ttttaattgt ttgataagtt cccctgccat ttttattcct tcacttaccc
    36781 agttttcttt tccagctgct cttaatcttt cgatttgttc atctggaaca aatattcctg
    36841 gtacatttgc agtcataaat ttagccattc ctggtgattt taaaggtact attcctgcta
    36901 ataccttgca atccatatcc cttgttaatt ctctaaactt tctcattgta tttatgtcat
    36961 aaagtgcttg tgtttggaaa aatttagccc ctgcttttat tttctttttc atttttaata
    37021 attgaacttc tattggagaa tattctggag ttactgatgc tcctagataa aaatctggag
    37081 aaccttttaa cttattacca gccatgtccg tacctgccat taaagtctca gcagtttgaa
    37141 gtatccccac agaatctaaa tcaaacactc ccttagcttg tggatggtct cctacgctag
    37201 tatggtctcc tgttagtgcc aacagattgt ttatcccaaa tacacctgct gacaacattt
    37261 ctccttgtat agctattcta tttctatctc tccctgttat ttgaataact ggctctaatc
    37321 cagcatcttt taataattta catgtagcaa gagaagttgc tctcataact gccgattgaa
    37381 agtctgttac atttgctgca tgaactcttc caactaacgg ctttgcacat tctattaaat
    37441 gtgaaaggtc agtccctttt ggaggagcca tttcagtagt aactgcaaat tttccacttt
    37501 ctaatgtttc tcttaataag ctcatctcta atcccccttt taaccttcta aaaaaacttt
    37561 tatgaatttg ctgctgcttc tttctttttc tttgcactta aacttcttgg attattttgc
    37621 ttagaatagt cttttggtgg tcttatttct gctaagttat ctaattgtcc tatagcttct
    37681 agtctttcat atatctttat ccaagcacaa tcattctctg agtttacttc acacttacca
    37741 tttttagctc caccacaagc cccattcata agacctttag cacacatagt tactggacat
    37801 atcccacctg tccaaccaag ttcacagtct ccacaagcct tacaggcttc ttcatattct
    37861 ccaactcttt gtacttcccc tataaacaaa gtattgttgg ctggataaac tggcttatct
    37921 tttaaattct taactattgt ctgtgtacca tctccacatg ctaaagataa aactgcatca
    37981 gcttccttag tctcttcttt tagagccttt aaatcctttt tagactttaa aaggttacaa
    38041 gcagggtcaa gcattacata tcctaagatt tttttgcctt caccttcaag agtttccttc
    38101 atttttaaaa cttcttcttc cccaccactt ttacaagttg ctgcacattg attacaacca
    38161 actaaaacca gcttgtcaaa atcttttaag taccctagca cttcttctaa tggtttattt
    38221 tcagaaatta tcatatctac ttccccccta aaccttccac atatatatat ctatttattg
    38281 ttttgtaatt tgcaagcctt aactacatgt tttgcaagta tagatgttgt aactgaacca
    38341 acccccgctg gaactggagt tatcattgat actttgtcta aaactgcatt tgtatcaaca
    38401 tctccacata aatttccttc ttcatcaaca tttataccaa cgtctataac taccgctcca
    38461 tctttaacaa aactttcatc aaccatctta gctctaccaa tagctgctat tagcacgtct
    38521 gcctctgctg ctacacctga tagattttta gtttttgaat gacatattgt aacagttgcg
    38581 tgttcactta ataaaagcat tgatactggc tttccaacta ccattgatct tccaagaact
    38641 gttaccttac ttccttttaa atcaacatta tagtgtttta atatctcaac tactgctgta
    38701 ggtgtacatg gtggaaatcc acttttatcc ccttccatta ccttagcaga gtttattgga
    38761 ctaaaacaat ctacgtcttt ttccggtgca ataacatatt ttataacttc ttcatttaag
    38821 tgttttggaa gaggtctaaa acataatata ccattaacat ttttgtcatc attaactctc
    38881 tttaatacat caatatattc ctcttgtgtt atatcctcag ctaactctaa cacttctgtt
    38941 gttataccta tgttttgaca tctctttagt gctcctcttt cataagataa atcatcactt
    39001 cttgccccaa ctctaactat tgttagttta ggatttatac cttctttaat tagtaaatcg
    39061 acttctttta ttaattcttc actaatctta tcagctacag gttttccttt tataatttgt
    39121 cctttagttg acattccttc catacattca caactcccct acaagcaaac ttaaattaat
    39181 ttattctata taccacatat taataacata gtatctactt tcgtcattta aaaattattt
    39241 tccaagagct ttttcaactt tggcataaac ttcatcacaa atttttacac catcttcaac
    39301 taacttatct acttctgttt ttaccttatt aacatattct ttatcttgta tagaatttat
    39361 atttataact acatttaatt gtccaccaag tatagcagct cttaaacact gaactcctac
    39421 accaacatca cttatagcaa gtcttgaacc tttatcaact aaatcttcat gtaacttaat
    39481 agcttcatag caaactctta ctatgtttaa tggtacttca caagcaactt ttaatgcctt
    39541 ttccattgtt tctgctttta ttcttttttc ttcttctgtt tctttaggaa gcccgtaagc
    39601 ttttgataat ggtaagaaac attctgcatc atcatctatc atttttaata aatctttttc
    39661 taactttcct gccttactta atatctcttt tacactttct tcatactcag catacttctt
    39721 cttccctatt gtaaggttac ataccatact acctaaagcc atacctattg cacctactag
    39781 tgcagcagct cccccaccac ctggtactgc ttctttggaa gatagtactt ctacaaaatc
    39841 aacacaagtt ttatctgcta ttttcatgat aatcctcctt gaaaggatgt aggaaaatat
    39901 ctcaaattgt tcgagatatt ttacgaacta ttttaagata tctcctactc tttatttata
    39961 aattaatttc cttagaataa tccagctatt actccatttt catcaacatc tattttttct
    40021 gctgctggaa cttttggtaa tcctggcatc ttcattattt ctccagtaag tgcaactata
    40081 aatccagctc ctgctgaaat tgttgcttgt cttacagtta ttctaaatcc tgttggtcta
    40141 cctaatttag tttggtcatc agttaaagaa tattgagtct tagccataca tactggaact
    40201 ttagtaaatc caagtttttc taagttagct atttccttat cagcttcagg agtataatct
    40261 acaccatctg ctccataaat cttagtagct atagcattta tcttgtcttt tatagataaa
    40321 tcatcttcat agcagaatct aaagttattt tcttcttcat ccaataatct taaaacttct
    40381 ttagctactg caattccacc ttctccacct tttgcccata cttcagaaag agcaacatta
    40441 acacctaatt ccttacactt gtcttcaaca agtttaagtt cagcttcagt atcaagtggg
    40501 aatctgttga ttgcaacaac tgctggtaat ccatatactt gagttatgtt ttcaacatgt
    40561 tttaataagt ttggaagtcc tttttcaaga gcttctaggt tttcgttatt taagtctgct
    40621 ttagcaactc caccattgta ttttaatgct cttacagtag caactattat aacagcatct
    40681 ggttttaagt ttgccattct acattttata tctaagaatt tttctgctcc aaggtcagca
    40741 ccgaaaccag cctcagttat tacataatct gcaaagtgca tagccattct tgtagctata
    40801 acagagttac atccatgagc tatatttgcg aatggtccac cgtgtacaaa tgatggtgtt
    40861 ccttcaagag tttgaactag gtttggtttt aaagcatctt ttaataatgc agccatagca
    40921 ccattagctt ttaattgtct agctgttaca ggttcacctt cgtagctgta tccaactatt
    40981 atgtttccta atctttcttt taaatcagat atatcacttg ccaaacagaa cgctgccatt
    41041 atttcagaag caacagttat atcgaatcca tcttgtctaa ctgctccatc acctttttta
    41101 cccataccat caacgatatt tctaagttgt ctatcgttca tgtctacaca tcttctccaa
    41161 gttattttct tagggtctat tctaagtgca tttccttggt gaatatggtt atcaagcata
    41221 gcagctaaaa gattgttagc agctcctata gcgtgaaaat ctcctgtgaa gtgtaagttt
    41281 atatcttcca taggaacaac ttgtgcatat cctccaccag ctgctccacc tttaacacca
    41341 aatactggtc ctagagatgg ttctcttaaa gcaactaata catttttgtc tagtttagca
    41401 aatgcatcag caacacctat agtagtagta gtttttcctt cacctgctgg agttgggttt
    41461 atagctgtag ttagtattaa cttagctttt ttaccagtct ctctttttag tagattgtag
    41521 tcaacttttg ctttgtattt accataaagt tctacatcat cttcacctag tcctaatttt
    41581 tttgcaacct ctctaatatc ttgaggctta gcttcttgag ctatttcaat gtcagattta
    41641 aatcccataa ctgaatctcc tcccacaacc taaatagatt taaaatataa tttttgtaaa
    41701 aattgtatta atatgtaaaa attatatttt ataagataat tatacatgta aaattactta
    41761 aaaactagct actaagctat taaaatattc aaacttaata gctagttatt tatctaatta
    41821 cttacttatt acctatctat tgcctatgtc taacctttaa ccaacttaaa atcccaaatc
    41881 aaaatttcta tctattttat ttatccttaa gtgacattaa tttatctttt attgctttaa
    41941 tctgttcttt cgttgtttca ctagaatcat atggagattg attagctcta tcagaatcaa
    42001 taacatcttg gttataataa ataaatccta aagattctcc atcttctaag ttttggatta
    42061 taaattcttc atcttcctta tttctaattt tatttcctac tacaaatact ttatttacac
    42121 ctatatcatg tccaagtttc ttaacttttc tataagtctg tagacttctc tctcctggtt
    42181 ctacaactac tataaaagca tctacacctt gggctgttcc tcttcctaag tgttcaattc
    42241 ccgcttccat atccataacc actacatctt tattttgtag tattaaatgt gagcagagtc
    42301 tcttcaaaag aacatgctct ggacatacac atccagtacc accagaatct acggttccca
    42361 atgtaagtaa tcttactcca ttgtattctt tacagaaatt ttcagggata tcgtctactt
    42421 taggattcat cttaaacatt tttccaaatg aacctacaga agcagctgtt ctatctgaaa
    42481 ctaatttttt catttctgat ataggtacta ttgattcata tacttctttt ggaaaaccta
    42541 gtgctaaagc taaattagca tcagggtcgg catcaacagc aacaactctg tatccgtctt
    42601 cagcaaacat cctagagagc attgaagaaa aagtcgtttt acctactcca cctttacctg
    42661 ttattgctat cttcataatc cctaccacct tttattacta tttgcttttt agtagttaat
    42721 tttacagtta aatatttttt atactctata tttttataca ctaaaaataa actacagcta
    42781 gtccttacag gaaccaatta aatttaccct actagctttt caaagcagca tgtatagttt
    42841 tattattctt taatattgtt tatattttat ttattcagta tattcattat attttgatgt
    42901 tctttacttt aatatttgac tttattcaac tttattcaac cagaaacttc ttataaaaac
    42961 gtttattcta taacttcact attttttaag cttcagcctt ttctgccatc ttttcttcaa
    43021 ctaatgcttc aaagtgaaca cgttttttct ctatatcttc tattattgta gctgctaact
    43081 cttttggatt tgtatttaca gtaaatgtag ctccaacttt atcttttaat tcactagtta
    43141 atatatccac tgctcttgaa gaaccagcta ttggaggcat tattcctaaa taagtgtcta
    43201 taccagaagc tacaacatag catcctatag caactgcttt ttctgacatc cactctggag
    43261 ctatacctac aactggaagg tcacacatat ccatatctaa gtaattagct gctgctccaa
    43321 ctaaatctag tatacgactt atatcaacac aagaacccat atgtagtact ggtgggattc
    43381 ctactaattt acagacagtt gcaagacctt tacctgcata tttttcagcc gcatcagatt
    43441 ccattaaacc aaacttagct gctgctgaag ctccacaacc tgtagtaact actattatgt
    43501 cgtttttgat taattctttc attattgtta cgtgagcctc atttgaaact cctttagcat
    43561 tgttacatcc aactacacca gctgctcctc ttaatactcc agattttaaa cagtctgtta
    43621 atggcttaac tgttcctgct gggtctatat gagagtttac aactctgtct aattgattta
    43681 ctactgcttc tacactatac ccaactgttg cgcctgattt taattcaggt ataagtactt
    43741 ttgatttatc tctattttta aagtttaaaa tagcttcttt aactattgct ttagcatcat
    43801 caagtgcttg ttcttctcta aattcaatat aagttgaacc tgttatctta gcttttggag
    43861 aagttgttac aaatttagta tggtaacaat ctgcaactct agctaatgca gggaatatac
    43921 attgaacgtc aactattaca gcttcaactg ctccagttat tattgcaagt tcttgttgat
    43981 ggaagtctcc tgctattttt acaccatgtc tcattgtaat ctcattacct gtacaacaca
    44041 tacctgctaa gtttattcca tcagcaccaa cttctttagc taaagctact aagtctggct
    44101 cttctgatgc taaaactatc atttctgata atgctggctc atgtccatgt aatattatgt
    44161 taactttatt ttcttctaat actgctaaat tcgcctctgt tcttcttggc actggagttc
    44221 caaataagat atcgcttaga cgagtaccta tcatagaacc accccaacca tcagccatag
    44281 aagttctaag actcatgtgt attaaactat ctatatcacc agtacatcct atatgagtag
    44341 agtgcattat tgttgcaatt tctctatcta tagctcttgg ctctatagcg tattctttcc
    44401 atattttttg tcttggttct ggtgcatttt ttataaatct taatgtccca aaaggttttc
    44461 caaattccat taatgcaatt tctgccactt catgtgctac atcatagata tctctacctt
    44521 ctgtctctac atcccattct ttagctaaag taattaactt agcttcgtct cttattttat
    44581 agtttccatt tggatcagct aatgctaatg tatgtgctat atctctagca tggtctgaat
    44641 gggcagctgt cccaccagca accattcttg caaagtttct acctactata gtatgctcat
    44701 ctgctccaca taaccccctt ggagtcttag ggcttattct acatggtccc attccacata
    44761 ttctacagca aacaccttga agaccaaaac cacattgtgc cttaaagcct tcttttctat
    44821 catacattgt ttctacgcct tcttctctag ctttagctat catcttctgg ctgtttaaat
    44881 caattgttaa cattttttca tccatattcc tatccccttt ttttgatttt taaaatcccc
    44941 caggtgactg acttcatgtc ataaaataat aaaataaatg ctttctaagt tgattatata
    45001 ctaattttca gtgctttgct ttagatttaa atattttttt aacaaaatta gataatacat
    45061 aaaaatttta ttttctacac ttaatttaag ttgattttat cgcttgtttt tggctatttg
    45121 ttaatatcta aacctacttt tttgatactt cagtttaatt aaattatgtt ttgatttttt
    45181 taaatcaaat ttgaaatttt tctagtcatt tttatattat tgcagattgt ttgtgtattt
    45241 attatctttc actactgttt ggacttatta cagattttta ctccctaaac ttaattagta
    45301 aaaacttctt agaatagcat tttttggact aatttttttg aattttttta tttttcctac
    45361 tttaatatta attatagctt caaattgaac atttgtatat attcatttga agctataatt
    45421 attttttata taaaactttt ttaatacagc attaatttag acattttgac tattttttct
    45481 actttaattc atttatttca agcgatttat cctatttcat atttttagct acaatagccc
    45541 catatgcaat agaatataat ttagattcat gtgaatcagc tcttgacact aaaactatag
    45601 gagcttttgc acccataatt atacctgctg atttagagtc tgccatatat gtaaaggctt
    45661 tccctattcc attaccaacc tctattgttg gaaccaatat gatatctaca tctccagata
    45721 cttcactttt aaaccctttt atctcactag cttcttttga aacagctaaa tcaaaagcca
    45781 gaggaccttc tacaattaca ttttcaccaa attctccttc tttacaagct tttgctaaca
    45841 tatctgcatc tacagttgct tgcatcttag gatttacttt ctctttagct gccaaacatg
    45901 caacttttac agtttccatt cctaaagctt ttgcagcttt tatagaattt tttaatatct
    45961 ttgccttttg ttcataatca ggagctatat tcatacctcc atctgtagtt ataagtaatt
    46021 tatggtattt ttctaactca tataccataa catgacttaa taaactatca gttctaagac
    46081 catagtcttt atttaacact tcttttaata tgacagatgt atctaataat cctttcatta
    46141 caaagtcggc ttctttactt gatactaatt taactgctat ttctgcacac tctttgatat
    46201 catccttatc cactattttg ataccactta aatcaaatcc tatttctttt gatatttctc
    46261 ttattttgcc ttcatttcca accaatatag gagtaattat atttctttct acagcatcct
    46321 ttattgccat taaaacttct ttatcatgag ctgctgctac agagagtaca attttctctc
    46381 ctcccttaag ttgttctaaa atatcatcta atttttttat caaaagtaac ccctccctta
    46441 tatatattta actaaataat accatatttg ctataccttt tctcaattta ttttttatgt
    46501 tataaagaat ttatataact aaaagacagt ttaatatttt attctgggat ttcatcaata
    46561 actcttaaaa gctttgcagg aacaccacct acaatagtat tttctgcaac attttttgtt
    46621 acaactgctc cagcagcgat aattgcatta tctccaatag taactcctgg aactacagtt
    46681 acattagcac caatccatac attttttcct attgtgattg gagaaggata agttgtacca
    46741 cgtttctctg gtgcaatacc atggttaagt gtagccaata ccacattatg cccaatttgc
    46801 gtattatctc caatctcaat ccccccctgg tcttgaaaat ggcaactgct atttataaaa
    46861 acattttttc ctattgtgat gttttttcca caatctgtat aaaatggtgg aaacataaaa
    46921 aatgattcat ctaccttttt tccaattaac tcagaaaata aactacggac ttcttctggt
    46981 tcatgataag aagaatttaa cttacttgtg attttcattg cttcattact caacttaacc
    47041 ataaattcat gtatttctga tttactagaa acaggttttc tttgattgat atgtgttaaa
    47101 aattcattga tattcatttt atacatcctt tcaatatgtg ctttaattta tttgtacatt
    47161 ctaaataata ttttaataaa aattaattta atttattttt attaaaatat cattgcttct
    47221 tttagtgtaa tatacaatta tatctatatc aaatatctat agtaaatact taactatact
    47281 taaaaagcat ataaattgat ataattatat aatagataag ttaatattga ggagggataa
    47341 ttatggaaat aagagtatta agatattttt tagcaattgc aagagaagaa agtatatctg
    47401 gtgcggcaac ctttttacat ataacacaac caacactttc tagacaaatt atggaacttg
    47461 aagaagagtt gggagttaaa ttattggttc gaggaaatag aaaaatcacc ttaacaaatg
    47521 aaggtaggct tttacataaa agagcagaag aaattatatc attagttgat aaggttgaat
    47581 tagaatttga caatcccaat gagattatta gtggtgatat atatatagga agtggtgaaa
    47641 ctgaagctat gcgacttata gctagaactg cccatcattt tcatcaaaaa taccccaatg
    47701 taagatatca cctttttagt gggaatgctg atgatgtgac tgaaaaatta gacaagggac
    47761 tgcttgattt tggtatttta attgaacctg caaatataca gaagtatgat tatgcacaaa
    47821 ttcctgctac tgatgtatgg ggtttactaa tgcgtaaaga tagtgccctt gcatcacaaa
    47881 gcacaataaa tccaaaagat ttatgcaata tacctcttat tatttcaaga cagacacttg
    47941 ttgataaagc aatttctaaa tggcttcaag acagtaatga aaaactaaac attattgcaa
    48001 catacaattt agtttataac gcttctcttt tggtagaaga aggtctgggc tacgctctgt
    48061 gtttagatag actcgtcaat actacagaaa acagtaatct ttgttttaga cctctatctc
    48121 ctcctttaga agctaaatta aatattatat ggaaaaaaca tcaaatattt tcaaaggcag
    48181 cagaaaaatt tttacttcaa atacaaaaag atttttcttc tatcatgttg gatacatcta
    48241 agttggatac acctaaagaa aaataaatca taataaaagg tgagagtgtc tcaaaatgaa
    48301 ctttgcactg tataaggcac tcttttttat acaaagtaaa atatatgttc atcatttcag
    48361 cactaaaaaa gaggcttatc tctattttga gatagcccca cttaaccaat aaaattaaat
    48421 acaacttaac aatatcctta cttatttatt ctaagttctc ttagacaagc aatagtaatt
    48481 tcatcttgtg gatttaattc ataagctctt tctatatagt aagttgcatc atcaatatca
    48541 ccattattta aataagccat tcctaaattg caaagtattt ctgggtcttc ctttatctta
    48601 gccgcttgtt caaaatactc tattgcttta tctaaattat tcttataagc ctcacaaagc
    48661 cccaattcca ctatagtatc cacttgatta ggctttatta tcaatatctt ctcaagatac
    48721 atcttagcct gctctatttc gcccataccc ttatatccaa gtgctatcat aaacagcaag
    48781 ttccaccaat ccatatgttc ttcttctaat ggaagtaact tctctaatcc ttccttaaac
    48841 ttaccttgga atacaagtgt atatccttct tcatattgaa ctttaaagtc catcttgccc
    48901 agattctctt gtacctcagc tactaaatca gcatctaacc ctagccttaa cgcttcttcc
    48961 caagtcagct tagtctttaa atattgacct tgattataat aatggtagcc taagtgataa
    49021 taacctaatg caaaattctc atcaacatca attacctttt caagcttagc catggcttca
    49081 agtaagaatg cattcattgc cttatcatcc atatcttttt gatactgttt agctatctct
    49141 tggcaaacaa tcgcatagtt atacattgca tctaaatcat ctggatacat tgttataaat
    49201 gcttttatat atattaaaga atctgtatgt tcaccaatct caaagtattt tctcgacata
    49261 tatcccatat acgatttcaa atccaaatca atatcttttg ataaagcatt taaaaacttc
    49321 tcatactctg cattgttttt aaacttacta tctattccaa ttatatatat cattgcatct
    49381 gctatagaca ttgaatttaa cttatcttga gcagtctttt cttttatccc ttttactaat
    49441 acctcatttt ttataggtac atctaatcca ccttttggca cttcatagct ttttaattta
    49501 aactctccac catgttttat agttataaaa gctaattctt ctgctttttt tagcaaatat
    49561 ttttctatct taaaatccat tttatcctcc taaatactta aatctctact ttatgagttt
    49621 tagatttctc aaatattttt ttatcatcat actcaaataa gactacattt tctacttctc
    49681 ttatttctgt atttatatca ttttttaaat ccacaacatt aaatctaaat ggctcaaaat
    49741 gaacatattt tattatatgt ttagctggtt tatcaacaaa actaggtaaa tatgttgata
    49801 agttatcatt atcttgtaaa aatacatggc atctattctt aaaatcatcc acatctagct
    49861 tattaaaaaa ctgtggtata tttgatgttt tacccagtga tatatagtca tattttaata
    49921 tatcgttgaa tacatcatta tctatgttga ttttttcatt atagaaatcc atcaatattt
    49981 tatataactg attctttcct tgtgccaaat caaagtagcc attttcatca aaatacttgg
    50041 caaaaacctc aaagaattta aaagggcttt ttttataaaa tctgcctata atatatctca
    50101 tagataacac aaagttttta gaattgtagt atctctccag tatatcttct atatccttta
    50161 gcttcaaagt ttcttcatag cttatataat tattgtataa aaactcatat ggagcataat
    50221 ccttatattt aaaaccatgc tcatctgcaa cttttctcat acctgtccct ttaatcatct
    50281 ttaaaaagcc taattgcaaa tgctctatac ctagattaaa tacatcatta aaagaatttt
    50341 caaagctctt ataatcttca taaggcagtc ctgcaattaa gtctaagtgc tgatgtatat
    50401 ttctataaga tgctacagtt tgtacaacat gagacaactt tttaaaatca tcccttctac
    50461 ctactgcatc aagtgttttt tcattcgttg actgaacacc tatttcaaat tgaaaaagac
    50521 cttctttaca atcagccaaa aactcaagca tcttatcatc tatcaaatac gctgtaactt
    50581 caaaatggta agttgtatat ccattatcat ttttcataag gaaatccatg atttccatag
    50641 ccacttttct atttgcattg aatgtcctat ctataaactt tatctgtgat actcttgcat
    50701 ctattaaagc ttttaaatca ctctttatcc tgtctaaact aaaatacctt agacctggaa
    50761 tagtagatga taaacagtat tggcagttaa aagggcaacc tcttgatgat tcataataca
    50821 ctattctatt ttcatattct tttggattca agttttcata aggactagga atctcatcca
    50881 aatcttgaag taattccata ggcttattca ctattatctc attgccttgt ctatatacaa
    50941 gtccatttac atcatttatt tccatctttc cttgcaagtg aagtactaag tctctaaaaa
    51001 ttaattctcc ctctccataa agtatataat ccacaaaatc atactttttc attgcctcat
    51061 aactatcata actcacttcc ggtccgccca aagctatttt agtattaggt ctaatttttt
    51121 ttagattatc acaaagttta actatatctc ccacattcca gatatatgtt gagaataata
    51181 ttatatcata ttcattttta tatatatcct ttaaaatata gtccaaatcg ttatttatcg
    51241 tgtattcttt catattaact tctatcaaat ctcttacaaa ttcttttaga tatcttatag
    51301 ccaaatttgt atgaataaac ttggaattta atgttgttaa taaaattttc aaatatttct
    51361 cacctctttt taagattata ctgtcttttt gtacccctta taggttaaaa tagtatatgt
    51421 aaagtctaaa taatatctat ataattaatt tattaatttc aagtaatgtg aggaaaggaa
    51481 gttttttatg gaattaattg aaaaagtatc tatcaactct ttatcaaaaa gagatttact
    51541 acttattata aagtctcttg aatacactaa tgaaaatact caaattagtg atttcattga
    51601 ccttaggaat aatatagtaa aggaattatg ttttttaact gacactaagg aaaaagattt
    51661 tttagattat ctagaaaaaa atagcacact ctaataacct aaaccttttt aaactttaat
    51721 atataaatac ctgatttaga atttattttt atgcttaata tattatcatt aaaggttttc
    51781 gacataactg ttatttgatt aaatataggg tctattatag tattaccttc ttcaagtctt
    51841 ataagacttc ttgggtctat gcttttatta tatgccttaa ctccaatttt atattttctc
    51901 aatttaacaa catattcgga catagaaaaa ataaaatcta tattatcttc atttaattta
    51961 ctatcttcac ccaaaatgga taatatagat ttttttatgc tattaattcc ttcattttct
    52021 acagttctat cttccatata tacatcatat agaacattta aaacatttaa taaaatactt
    52081 tcttcatcat aatcttcaga tttcaaatca atgtacttat caattaattg tattgcttta
    52141 atccttgcca tctgaaactt tacaacatct gatttgtcta attctataga aaatcctatt
    52201 atcttgtctt taatactctg tagtaattcg acatactcta ttttactatt atttataaga
    52261 ttgtgcataa tggaattata caccacagaa cctaataaat aatcaaataa aagttcttct
    52321 tttttaaaat aaataacata cttttttatt agtttaataa cctcatcttc acttttttga
    52381 atattagtat ttgataaaat tagtggtaat aaaatattta aaaaattgat atttcctatt
    52441 ttttctccat aaaatctata gtaaataagt tcatctctca gtctattttc gtctatatac
    52501 acacatcttt gcgttttatc atctattaag gattttgtat ttattatagt taaatcttta
    52561 agagccctac ttgcactact aaaagaagat gtgtagactt tttttagtaa ttcgtcattc
    52621 ataaatatat cctttctgaa aaattgaatg taaataaatt gttattaaaa aaaaagagac
    52681 agcccgttta agaagctgtc atatataggg ggatagtagt gtacaaaaat cattgtaatc
    52741 tactatctat atattattat acgatactac ttttttatat gtaaagcatc agttttcgac
    52801 atccctatat tttacagtta tacaaattgc gtactaagtt caattttatt ctttttattt
    52861 caacatttta tcatgctaca ttctttcagg agcgtgcatt cccaacaatt tcagagaatt
    52921 ttctaaagtt tgttttgttg ctaaaactaa tgcaagtctt gcttttctta tttccaaatt
    52981 ttctactaaa attggattgt catgataaaa tttattaaac gcttgagcta aatctagcat
    53041 aaatctagtc actatatgtg gctcattttt ctttagagca agtaaaatat tcttattgaa
    53101 tgattctata actctaagaa cttctgcact atcttcatca gctaatagac tataatctat
    53161 atcagaagtc acttctactt ctgcttttct taaaactgca cagcatctag catgtgtata
    53221 ctgaacatat ggtccagtct caccatcaaa actaagcgtt ctttcccaag agaaggtata
    53281 gtctttaatt ctactattag ataactcttg gaatactact gccccaacac ctacttgctt
    53341 agatatttca tccacatttt tagcatttgg gtttttagct agtataattt ccttagtttt
    53401 atctatagct tgatttaaag catcttctaa aaatactact cttccttttc tagtcgacat
    53461 agtaccttct tcaagtgcaa ccataccaaa tccaacatga attaaatctt ttgcccaatc
    53521 atatcccata agctcaatta ccttaaacca ttgttggaaa tgtaagtttt gttgagaacc
    53581 aactacatat atacacttgt caaaatcata agtttctttt ctatatattg cagcagctaa
    53641 atctcttgtc atatataacg ttgagccatc attcttagta ataagtgctg gtggcatttt
    53701 atactcttct aaatcaacaa ttcttgctcc tttagactct tttaaaagat ttttctcttc
    53761 taatagttcg ataactctgt tcattttatc agaataaaaa ctctctcctg ctaaagaatc
    53821 aaattctata tctaaaagtt tatacactct gttaaactct tttaaactct catttctaaa
    53881 ccattgccat aattcttgtg cttcttcgtc accattttct aatttattaa accatgctct
    53941 tgcttcatct tccatttctg ggtgttgttc agcttcatca tggaatctta cgtataattt
    54001 tagtaattct ggaattggat tagactctac tacttctttt tctccccact ttttaaaggc
    54061 cactattaac ttaccaaact gagttccata gtcacctaaa tgatttattc ttatagtctt
    54121 gtatccttgc gaatcatata tcttatataa agcattacct ataactgtag ttcttatgtg
    54181 ccctatatgg aaaggttttg ctatatttgg agaagaatat tcaactatta cagttttatc
    54241 ttttccaaaa tcactatgtc cgtaattttc tttttcatct aaaacttttt taattacagt
    54301 ttctgctaat tgagatttat ttacaaagaa atttacatat ccgcctaatt gtattacttt
    54361 tgttatctct ccacttggtt ctatactttc cgccaattca cttgctatca tgtttggagc
    54421 ttttctaaac actttggcta atttaaagca tggaaaagca tagtccccca tatccttatt
    54481 tggtggaact tctataagtg cctctatctc ttctttcgat aaatcttcta ttttttcttt
    54541 aaggcaattt gatattgcca ccttaaaatc ttgcattctg ttgctcctct ctacattgtt
    54601 cctggtttat attgtgctta tctctttatg ttaaatcaaa tttcgtgttt tttcaagtat
    54661 ttcattacag tgtaaaacac tttagtaatt atgttttctg tatatattaa aaatactata
    54721 attgcagttt ttaatcacac tattattgat tttttataag acttttttct attttattgt
    54781 ttcttgtact atccagttga ctttatatac tttttttaat cttttatcta ttttcatacg
    54841 tcaatgctaa ttttatatac ttttctttta atcttttatt aatttttata catcaatgtt
    54901 aactttatat aagttttttt aatcttttat ctgttttcat acatcaatgt cagctttata
    54961 tacttttttt acttttttat ctatttttat acatcaatat tagctttata tacttttttt
    55021 aatcttttat ctattttcta taaatctttt tcactcttaa cttttattcc ttcttcttct
    55081 aatagccttg ctgttatacc tttccctggt atctttttac ctgaaaaact accatcatat
    55141 attagattac atccacaaga ggggctaccc tcttttagta ttgcttcgct acatacatat
    55201 aaatttgcta tttttagagt ttcattagcc cctcttacaa agttttctgt tacatcctta
    55261 ttatctttag taattacttt tccatcaact atctcagcag gtggtcttgg tgtagataga
    55321 ccacctaact gctctgggca tacaagtgta aattctttgc cttttaaata ttccttgact
    55381 ttttcattat cattattatc gccactatat ttacaattta ttcctattaa acatgcagat
    55441 accaatatca taaaaatgtg ctcctataca aattcttaat atattctcat acaaattact
    55501 aatgtattct tatacaaatt actaatgtat tcttatacaa attattttaa tttaacaatt
    55561 gttactcctg ctccaccttc tccatattga ccacctcttt gtgattttac atgtctattt
    55621 cttcttaaaa tgtcttgtag ccctgctttt aatactcctg tacctattcc atgtattact
    55681 gttacagact ctaaacctgc cacatatgca tcatcaagat acttttctac ttccattata
    55741 gcttcttcta ggtttaaccc tctaaggtca acttcatttt ttacactacc agattttgac
    55801 cttataatgt ttctcgttga tttagttaca tttgttgaaa catctttgcg tgttttttgt
    55861 aaagacttaa aaggtagagt catcttcatt ataccaattt gcactactgc ttcttttctc
    55921 tttttatcta ctgaaactac actaccattt tgatttaaag ttattacctt aacttcttca
    55981 cctggcttta agtcttttat ctccttattt gatacctttg gaactatcat actttttact
    56041 gttggctgaa gacttcccat agagctggtc aattcttttc taagttcttc tatcttacga
    56101 ttcttttctt ttgaagctct ttcttgttca agacttctaa gttcttttat tataatatct
    56161 acttcttctt tagcttgtct tataattgaa aatgcttctg atttagcttt ttcaatcatc
    56221 ttatctcttt gactgaccaa tttttcaagc ttttcgtcat actctacttt cagtttttca
    56281 atctcttctt ttaatctttc agcttcttct ctatcttcta ctgctttgat tctattcttt
    56341 tcaacattct gtaacacatc ttctaaagct atgttttctg tatttatata ttctttagca
    56401 cgtgaaatca cataatcact tagcccaagt tttctagaaa tctcaaacgc atttgatttt
    56461 ccgggaacac ctataagtaa cttatatgtt gggcttaaag tttctatatc aaattcaact
    56521 gctgcatttt ctacacctgg tttagtaaga gcataatttt taagctcact atagtgagtg
    56581 gtagctatac atttagctcc tacagaattt atatcttcta atactgctat tgcaagggca
    56641 gctccttcaa cagggtctgt tcccgctcct aactcgtcaa atataactaa agagtctgct
    56701 gttacgtttt gtaatataga tactatattc gtcatatgag atgaaaatgt tgatagactc
    56761 tgttcaatac tctgctcatc tcctatatct gcaaatacat tatcatatac acacatacta
    56821 cttccataat cagcaggtat atgaagtcca ctttgtgtca taagtgcaaa tagacctaca
    56881 gtttttattg taacagtctt accacctgtg tttggacctg ttatcaccaa tgtatggaag
    56941 tctcttccta aataaattgt atttgctact accttctttt tatccagcag tggatgtctt
    57001 ccatttttta tgtttaaata tttatcctca tttaaagttg gttctattcc tctcatctgt
    57061 atagaaagtt ttccctttga aaatgcaaag tccaatcttc caagtatctc ttgattagaa
    57121 attaagtctt cactaacttc tccaaccata gcagaaagct ctgataaaat tctttctatt
    57181 tcttcttgct cacctagttt taactgtctc aattcattgt tcatctctac tatagtcatt
    57241 ggctctataa aaagtgtcgc accagaagat gactggtcat gcactatacc agctacttgc
    57301 gacctatatt cagattttac aggtactaca aatctatcac ctcttagaga aattattgca
    57361 tcttgtaagt acttttgata agtagttgaa cttattattg aatttaattt tgacctaatg
    57421 gattgatttt tttgggctat tcttcttctg atatctctaa gtgtacttga agcattgtca
    57481 gatatttcta cttcactaat tatagcattg tatatctggt cttctatgtc cttgtataca
    57541 tatagagaat ttgatagaga ttgtattatt ggataattaa aatcttcttc atcactacta
    57601 gataagctat ttcttaatct tctagccact cttaaggtat ctgctatcat aatcagactt
    57661 cctgggtcaa gagaagcacc tatataagct ctctttacct tatcttctat atcatgaata
    57721 ccttccaacc ctactgaacc tctctttata ataattgatt gagcttcact agtttcttct
    57781 agcatagatt tcacttctac aaaatcagta tttggtacta aattttctat atattttaga
    57841 cctaaagaag atgaggcttt cttctttaac aaatctatta ttttattgta ttctagaact
    57901 cttaacgatt tctcattcat tttctatatt caccatcctc gatattttaa cttttttatt
    57961 taattttaaa aatgtatgtt ttcaagcatt tccaataaaa actgcaagtt ttatttcttt
    58021 ttcatttcta aaattattat attttttctc ttttagatat attttacaca tattttgtat
    58081 tatactatgt atgtagctat ctatatatta tacataaaca tgctttattt gtaatataat
    58141 ctgttaaaaa atcttcttta atatttttaa aggagtttat tttagcttat aaaaaaaaat
    58201 agctacttat atagataaat taaggaggaa catgagtcat gggaaacgta ttagaaggat
    58261 taaaaccaga aagtgttttt aaaaactttg agaaaatatc tcaaattcct agaggttctg
    58321 gaaatgaaaa aggcataagt gattttttac ttagctttgg taaaaattta ggtcttgaaa
    58381 ctattcagga tgaaagctta aacataataa taagaaagcc tgctacaaaa ggatatgaaa
    58441 attgtccagg tgtagttctt caaggacata tggatatggt ttgcgaaaaa gaaaagaatg
    58501 ttgaacatga ttttcttaaa gaccctataa aattaagaat tgatggtgac atgatatatg
    58561 ctactggaac tactctagga gctgacaatg gtatagctgt tgctatgggt atggctatat
    58621 tagaagataa tactttagag catcctgcac tagaagtttt agttactgtt aatgaagaag
    58681 atggtatgaa tggtgcagat gcactagacc ctagcttaat taaaggtcaa tatatcctaa
    58741 atatggactc cgaggaggaa ggatacttat tagttagctg tgctggtggt aaaacttgtg
    58801 ttgttagttt acctgttgaa tacaaggaag ttaaaggtga caaacaagga ttattagttg
    58861 aggttactgg actattaggt ggacactctg gtatggaaat tgttttacaa agagcaaatg
    58921 ctaacaaagc aatagctaga gtattaagtg tattgaatgt tgattatgaa ttagcttctg
    58981 ttgatggtgg tactaagcac aatgctatac ctcgtgaagc taaatgtgta atagctgtta
    59041 ataaagctga tgttgaatct gctaaaaaac aaataaatga cattttaact gcatttaaac
    59101 atgagtttac tacttctgac cctggcatga cttactcagt agctgaaact tctgtagaca
    59161 aagttcttac taaagattgt aaagaaaaag ttgttcaaat gtcttgctta actcctcatg
    59221 gagttcaatc agtaagtctt gatatagaag gattagttga aagttctact aacttcgcta
    59281 taatcgaaac taaagaatct actattgaat ttttaacttc tgttagaagt tctgttatgt
    59341 ctattagaga tgaaatcgct gatagaataa gacttttggc tcaagcttta ggtgctaatt
    59401 acgaccttat agctcaatac ccagcttggg aatttaaaaa aggctcaaaa ttagaaaaaa
    59461 tatgcagtga aacttatgag aaattaactg gtaaagttcc tactgttatg gctctacatg
    59521 ctggtcttga gtgtggatta ttacttgata aacttcctca tgctgaagct atctctatag
    59581 gaccagatat gtttgatgtt catactccaa atgaacatgt tagtatccct tctgtagcta
    59641 atgtttggga ttatgttatt gaaatattaa aatctatgaa ccaatattaa atttaagttt
    59701 tgattctaat tagattttaa ttttaagtaa tctatataaa attaactctg taaattaata
    59761 ctactgttag tataatattt actttcaata ttaacttata gaaataaagc ctaatgatag
    59821 ataataacat gtatcattag gctttattgt aatttaaata ttattaagct ttattctgtt
    59881 ttaaaattaa ttcttatact attttaaatc tatttcttcc ttgtctctta gaatcataaa
    59941 gagcattatc tgctatttga tacagttcat catactcttg accaggcttt gctataacta
    60001 cccccatact aatagataaa ctttctccat ttggcaactt aatgagatga atatgattgt
    60061 atacatcatt acatttatta ataagttctt ccttatttat tatatcattg atataaacac
    60121 taaattcatc tccacccaaa cgacctacta tatcattttt tgcaaaactt tcatcaagta
    60181 cctttgcaac atcaataagt actttatctc ctaacaagtg accataagta tcattaatcg
    60241 acttaaaata atcaatatct ataatcaata aagcaccatt tttttcagtt gataaattca
    60301 acaaattttg tgcagttaat tttctaatat atgctgcatt ataaatattg gttaaactat
    60361 ccctttgagc cttatctaac aatctattct tttcctcttt ttcagaatca atattacata
    60421 ttttcccgat tacatacatt ggtatttttc tatcattata aattattttt gaagtaactc
    60481 taatccaatg aaaagtatca tcaagcataa tagaatacat ttcagttgtt ccattttctt
    60541 gtgattttat tacatcaaaa aactttttat tatattcttt ccttatttta tctctttcat
    60601 tttctatatt gccattttca ctttccttat catttttatc caaaccataa taagtctcaa
    60661 tacccttttc tggcatagaa attatcaatt tatcttgctt ataatcatac tcaaaaaagt
    60721 attcacttgt catttcatac aactgagtat gttttttgag ttctaaagat atcttctcgt
    60781 tcatacttgt acgagtatgc aaaccaaatg ccataaatcc aataatacaa attaataaac
    60841 tggctacaat aaaaatagtc tcaattggat tctgttgcat aatataaagc aacgagaagt
    60901 cttgtctata tgtagtattt tggtagacaa ttgattgcat tttttctgat gaaatactta
    60961 taatagcttt attaaatata ttcaataatt cttgtttatc ttttttaatt ataccaaaac
    61021 aaatactctt tgctttataa gtttgaggaa ctagacgcac atttttatac ttaggttgat
    61081 tcatatagta ctgcatagaa taaccttcgc cataagtgta atctgcatag cctttattaa
    61141 cagcttctat acactcttct aaattatcat accaaactat attctcttca aactgaccat
    61201 aataattaat acctttggct agagctacac gttttccatc taaactattc tcatttaatt
    61261 tattattaat catcatagta tattgagtag aaagaaaagg ccgagacata actatattat
    61321 gttcacgaga gtattcataa tcataaatca ttcctgctac aatatcaatt tcacgctttt
    61381 tagtcatgtt ttctaaatct tcttgagatt ttacaggcac aagttcaaat tctaagcctg
    61441 ttttatcaca tatatgattt aacaaatcaa ttgatatacc tcttaattca cctgtatctt
    61501 tatctatatt ttgaaatggt ggtttgttta aaagtactcc tacttttaat ttttctgtct
    61561 ctttaatgta atcagtttct tcatcagata aaaacatttg attgttctct ggtgtaaagt
    61621 atttttcata taaagtagtt gaaaaatatg ggtctttctg ttcaatatta ttaattgctg
    61681 agttaagctt ttgagttaaa tttgtactac ctttaggagt aatgaaataa aatggttttg
    61741 atgaaaattt tgctacagta cgtacacctt ctagtgggtt catactcaca ttaagaagta
    61801 catctgcttt accactcttt agtgcttcta gttgctcatc atttgtttta caatatacca
    61861 attcaggtga cataagattc atcttacaat actcatctaa ctctttaagc ctagtatcag
    61921 aattttctat tacagcaata cggatttttt tcacatcttg tgaactcatt atgttatcaa
    61981 tatctgtatt ttcatataat gcttgtagta ctgtatttgc tactccataa ttattccctg
    62041 cataattata tattttttgc attggctcat tgtataacat acctcccatt atatcaattt
    62101 caccattttc taacatcttc attaattttg taatagattc atttatatct ccaggaactt
    62161 gaacaaattc ataattccat ccagtatact gtgcaatttc tagtaaatat tcatatgtat
    62221 acccaaaata gttaccttgc tcatctattt cagttaaacc tttttgaata ggataagcaa
    62281 cacgaatggt acgattatca ttacctatag cgttgatacc aaaagaaaat atgcaagaag
    62341 tgaatataga tgtaattaca ataattaata aaagtaattt ttttaaattc actacacaat
    62401 cctccatata gttaagattt acagtataaa ttactgcata aacttgtaga catgtaaaaa
    62461 tagattagta tataaaagga tatataaatc tacttagaaa tttaaatatc cttaatatat
    62521 acaatatatt taaattataa ctcatttata tgtctattac aacaaattta ccctgctaaa
    62581 tacctactct atatcccata attttacaag ttgtagtgaa gtttaagttc tatctcacct
    62641 caaatttagc agtgtttcta tttacagttc ttttatattt cacatctgga taacccaata
    62701 taaatgaagt gataacagct tcgttctcgc tcattcctaa cagttctttt atctttggct
    62761 ctacatttga tgccataaca aaaaatccat tataacaaat tcctaatcct tgagaatagg
    62821 ccataagttc catattagat gcagccagtc ctccatctac ataagcactt ccacctagag
    62881 acatatctcc tacaactacc ataagtagtg gggcatcata aaataaagaa tcatttccgt
    62941 tctctttata tcttttgtac attttcttaa atgtattttt atagatagaa cgtacagggt
    63001 cattgtcatc agtatctaat gccaaattat aaagaccttg tattgccagt tctttcactt
    63061 cttttagctt ttcttttact aatatgtatc ttataggctg tctattacca cctgttggtg
    63121 tgtatctacc agcttccact atgtttttta ttttttcttc ttctacctct atatctttat
    63181 attgtcttat acttcttcta aatttaataa aattcaataa cttttcgggt tctaattcaa
    63241 aactttcacg attgtattct attacttcat ttttatcaaa tccttgcatt tctattgcat
    63301 ctgttgggca aattgccata cagtgaccac agttaataca gagaatattt ttaacctttg
    63361 ctttatcatc cttaactttt attgcattta caagacagtc tgattcacac ataccacagc
    63421 ctacacattt ttctaaattt atgtctatca ttccaaatta ttcctccttt taaaaaattt
    63481 actttagtat aaaacaaata gttgtctaag tcaattttta tttttttaac aatatactag
    63541 ccttaactac actttaatgg agaaaaatat acataaattg gaacttaaat tagaacttat
    63601 tcaaagtaaa agataaaaga agtagatgtc taaaagtgaa ctttttagtt aattagatat
    63661 tctttattgt atgaaatcaa gtggatattt atcatttcaa caataaaaaa ggcttacctc
    63721 tattttggat atgtccctgt caagtagaca gactaaaaaa aggctatctt tatgcactga
    63781 gatagcccct tttcccaact actattgttt atcttaatat tagtcaataa atttaatttg
    63841 ctataacctg taagttacat tttaatttga tgtaacttta aattacattt ttaagtaata
    63901 tctttaaatt atattcttaa aatatatcat taaactacat tttaattaat ctaaaaacta
    63961 tatcttaatt aatctaagaa acttacatta taatctttta gtttcccatt atcatataca
    64021 atctttgcac taaagatatt tccagtctta gcccaattta agaacagcct gtcaccttcc
    64081 cacatattta aatcgaatat tttatcatta tcaacccaaa ttaaatttcc ttctttacaa
    64141 tcatttaaat ctttttcaga taattcacct tcaaactcat ctgagaaata caaaaacata
    64201 taccaatctt cttcatcttt aaattttgga aaagttatca accctttata tgacaatgat
    64261 ttaagagtaa gaccagtctc ttcttttact tctctaatta cacattcttc tggggtctct
    64321 ccttgttcaa actttcctcc aactcctaca tacttacctt catgtatatc atctttcttt
    64381 ttatttctgt acaacatcaa agtcttgtca tctttttcta tataacaaat agttgtaagt
    64441 ttcatgtatc tcgccttgct ttctctttat attctcaact aattattttc agcactttca
    64501 agcacatcac ttatattctt cattgcatta agagaaagct ctgcaaattc atcaaatgga
    64561 atccctatat cttctatact tctcattgca tctctattta catttgaagc aaatctcgtt
    64621 tctttcattc ttttaataac agacttagtc tttacactgg taagtttttt atctggataa
    64681 actaaagcta tagcagttat aaacccagtt atagggtctg ctgcatatat tgctttttcc
    64741 attaggctag ttcttgtaac tcccattcca tcatgatgac caagtattgc tctatacatt
    64801 tcttcgtcac cgaagttttc ttttttaagc agttctactg ttgttgtagc atgtccttca
    64861 catatttctc ctggctttct tccaactata tctgaatcca aatcatgtaa aagacctgcc
    64921 actgcccact cttcttcttt ttctggctct agtcttttgg ctaattctct cataacagct
    64981 tcaacagcat aacaatgttt tctcattcta tcagtttcta aatacttaaa taaaatttct
    65041 aaagcttctt ctctcatttt caccaatccc cctgtttttt cacatatatg atatttttat
    65101 atattaatac acataaattc aagtttttat atatattcat gtatattttt catgttatat
    65161 ctattctaca cctttatagt agtgtcctgc aactgttcca atttcatcta atttcttata
    65221 caagtctgta gccttttttc caagtttata attattactc aatacctcaa tgtgagcttc
    65281 aataaccttt ttaaccatct cactatcttc aactgtaaaa tctatcctaa atacatctat
    65341 tccaatttcc tctaactcat atagattgtc caacatacaa gtaaccttcg aattgtaaat
    65401 tgtacttcta cagaatatat cttgtgttat cctataactt gcatccgtaa agtctcttaa
    65461 agcataacta ctttccttac atttagcaac tctcttgtcc tttttacaat ctctaacaac
    65521 tactcccata ggacagtatt ctgtaaccat cataggagca tatccatata ttactgcttc
    65581 aatttctaca tctgtatatt ttaaagtttg tttaatttca tttaagttta gttcttgtga
    65641 caaacaaact gtacttgctc cctcttcttt gaagtggttt atactttcac tattgaatgc
    65701 atttaagtag caatctatat ttaagtcttt tttcttaaag tattccatag agccccagtt
    65761 accaatttga accttattaa ttccttttat attatccaat atctcaaatt gcttgtactc
    65821 tttatttcta acaatccttg gtgctgaata gattaatttt ttgttagtga ctgctgtatt
    65881 atagtggttc ttattcaaag tatctatttt acttgtattt gacaaagtat ctatgttatt
    65941 tgtatttgaa cccattagtt ctttaggagt atctaaagac gaagaagtat ctaaatccat
    66001 agaaatactt gaatctatag aaatatttga atctatagaa atacttgaga ccatttgtaa
    66061 agcttttgat aatgtattta tatcttcata atagattaaa tctacattat aatttaatac
    66121 tgcttctaat tgctccaagt tcttaacttt aactctaatc ttaggaactg tatcaagttt
    66181 tttctgttta tttactctat ttggctggta ttttaatcta ttcttcttaa attttctatc
    66241 ttttatcttt attctttctt catctaaaag ctcaatacaa tcccttctca tttgatttaa
    66301 cacacttata ggcatactta caccttcatc caggtcaatt tctatctctt ttaacttata
    66361 tggtgtattt ccaagctttt gaatttgtgt ttctattttt tctttactta aagccacctt
    66421 tatagctttt tcaactattt tgtctccttc taccaccgat ttatttccac aaaaatcact
    66481 tataaaaagc tctggctttt tacctagttt aatactcact tttgcacgta tattagtttt
    66541 tatgaactct ttatcttgct gaaatgtagc ttgtactcta tccatcaatt cgctgtcaga
    66601 agtcttatat ataagctgat ttctctttgc ttctccaaca aaatctaatt ctatcaaatc
    66661 ccctttatgt gctattgtag ttatctctcc atcctttatt attcttccaa tagtacctcc
    66721 acctaagtta attccatcac ctttcttaag cgtattttct agcattactc taagtctttt
    66781 gttctttcta ttataatcta aaacttttcc aatatatagc ccttgattat taggtctatt
    66841 agagttcatt atatccttac caacttcacc taatatatat ccttttgtaa actttctatt
    66901 aaatatggta tacaaattat ttattatttc ctcagaaata cttatttttt tcttatctac
    66961 aaattcatta atcgcttctc tatatcctga aaccacagta gctacatact ctggcttttt
    67021 cattctacct tcaattttta aagataatac acctgcttca attattttat ccaaatcttc
    67081 tatagtactt aaatctctag gactcaacaa ataatctcca ttacttttta ctacttctcc
    67141 tgtatttata tctatcaact catacttttg tctacatggc tgtgcacacc tacctctatt
    67201 tccagaccta ttgcctatca tactactcat aaggcattga cccgagtaac atacacataa
    67261 agccccatgt acaaaaactt ctatatctac agttgtattt tcacaaatcc attttatctc
    67321 atcaacattt aattctcttg ctaaaacaac tctatcaaac cctacactct gaaggtattt
    67381 tacatcttct aaggaatgtg ctaccatttg agtgcttgca tgtagttcaa agtcaggtaa
    67441 tagttttttt ataagtcttg ccattcctat atcctgtact attaaagcat caatatctat
    67501 atcgtataaa aattttacat actctacaaa atcttctatt tcatactgtt ttatcaatgt
    67561 atttacagta acaaagactt gcactcccct tatatgagag tatttaacag cctcaatcag
    67621 ctcatttcta tcaaagttat ttgcagatgc tctagcacta aagtctttac cacctaaata
    67681 cacagcattt gctccgtttt gaactgctgc ttttaatgaa tcaaaagagc ctactggtgc
    67741 aagtaattct atttttttca tatttttctc ctctatttat acttgttagg ttaatataat
    67801 tatatctata acttaaatat ttgtattaaa tttatatatt tcttactata ttttaaattc
    67861 ttcaatcaat ttatatatta gttatttata tttggttcat ttgagttaac atatacatta
    67921 tttactatat ttagtatatt tctattaact tatatattta ttactgtatt ttaaattctt
    67981 caattaattt atatactgct tactaaattt aaaacatttt aattaatttg tacactgttt
    68041 actatacttg aaatacttct attaatctat atattgccta ctatacttaa gacattttaa
    68101 ttaatttata cactgcttac tatattttaa attctttaat taatctatac aatatttgct
    68161 atatataaag tatatcaaat aacagcttca tttttttgta aatttacaga aagttaattt
    68221 tcattaaatg aatcatcttt attgataaac ataattccaa agaccactgc caaaataaaa
    68281 aaacatatta gtattgattt ttccacgaaa atttttgtaa aaaaagttcc taaaatagct
    68341 ccagatataa agaatataat aatagcataa tactgtaaac tcttttttat cataaaaata
    68401 ttcttagtat gtatataatg aaataaagtt tctgtagcac ttcttaaatt tccagtacac
    68461 attgtcgttg caaaactatt cccatttact tttctaaaac tctctacttg cattgaacaa
    68521 ataaaagaaa tagcaacatt tacaaacata ttaaatcttc ctctaggaat aaatgaaaca
    68581 ataagtattg taatcatttc aattataatg ataacctgac gccaatgaat ctttgtctta
    68641 ttatttgtaa aatactttct tatcttctcg gctataaata cacctaacat aaatgccaaa
    68701 ataggaatca aataataaaa tgattccttt acattacctt ctgctatatt aagcccaaac
    68761 aaaactatat ttccagtttg tgcattagca aagactttac ctcttaccac ataagtataa
    68821 atatccaaaa atcctcctgt tattgccaac aaagaacata aaaagaatga ttcagacatc
    68881 tgactcttgc ttgtcatata taaacctctc gctttctaat gatagataca aaatcaaaat
    68941 cattttagat tctatttttc attgatataa aaaaattatt tatatttaaa tattgtctat
    69001 atttatacat catttatatt tatttatatt taaacattat ctatatctat atagcattta
    69061 tatttataca ttaaaagaag ctgtctcaaa aaacaagaga cagcaaatct tttagtttat
    69121 tccacatttt ttgcatcatt atttaaccct gtacatttta actgtaaatc ataagctttg
    69181 ttttgaaact cagaagctaa gttttcagct atagtagctc tttcatttgc ttcttcaagt
    69241 tgagtactta actcctcaat cttgttttta taagcatcta actctgctct agaaccttct
    69301 gtcgtattac taagttcgaa tatttcttgc tttttatttt ctataatctc aattaactct
    69361 ttcatcttag tttcagcttc acgattttct gcttcttttt ctgctatcgt aagctttaaa
    69421 gatttgattt ctaattgaag ctcttcatta gatgctccaa ccttcttact caattcttca
    69481 ttcgctttaa tcaacttctc attctcatct gaacattcaa ataaaaggtc tgatatattt
    69541 aatgcagtta atatcgcagc aactgataag ctcaatttcg ggttttgacg tgttatttta
    69601 tccatttctt tgtctacaaa atcagcaata ctaatcataa acttctctga tttgtctcca
    69661 accattggat attcagcgcc atggatttta accattactt tgttcatcct aacctccccc
    69721 gacattagtt gttaatttga tcttaagtta gcatttaatt tttcgcttaa ttcacttaat
    69781 atcttatcat gcaccttagc tacatcttca tctgttagag ttttatcttt acttctataa
    69841 gttattgaat atgcaattga cttgtatcca gcttctattt gagaaccttt gtaaacatca
    69901 aataacttgt agctttctac taatccttga ccattttcct ttattatatc ttctatttgt
    69961 tttacaaata catcatcttt aacaataaga gctatatctc ttgatgtaga tggatatttt
    70021 ggtaatggca cataattctt tgttctatca gaattatcaa atacaaagtc aatatctatt
    70081 tcagcgacat aaactctttg acctagattg tagttttcta taacatctgg atgcaattca
    70141 cctaatgtac ctacatattt attattatac actattttag cacatctacc agggtggaag
    70201 gtagtattat tatcttgagg ttcaatttca taacctttaa atccgacatt tacaagtata
    70261 gtttctatag ttccttttag tgcaaagaag tcaacatctt taccatacat acctacacac
    70321 attctatttt cttccttagg taattctgaa tcttgtggga tgaatatatg tccacactca
    70381 aatgcagata cttcttctat tttatgagat acattagttg ataaaacatc taacatattt
    70441 ggtattaaag tagttctcat tacagaagtt tcttctccta atgggttaag taatttaaca
    70501 aaatttctct ttgcattacc ttctggtact cttatcttat ctactccctt agggcttaca
    70561 aatgaatatg ttaatatttc atttaatcct acagcagttg aattatcttt taaagcatcc
    70621 atgaattttt gttttgaagt tttaattcct gctgtcgtat ttccttctaa ttgtacagaa
    70681 ggtatattct caaatccata aattctagct atttcttccc atacatcagc ttcttgttcc
    70741 atatctgttc taaagcttgg tacatctatt tctaatttat cattagctac caaattacat
    70801 ttaaactcta atgattctaa aattcctaca aactgctcca ttggtacatc tacacctaat
    70861 aggtggttaa ttctttgagg atttactacc aattttttag gttctggttt atttggatat
    70921 acatctacaa caccttttaa aacttttcct gctcctaaca tttcaacaag ttgtgcagct
    70981 ctatttgctg ctatctctgc aagattaggg tctaagtctt tttcatttct tgaagatgct
    71041 tctgacctaa taccaacttt tttagctgtc attcttatgt tttctggttt gaaattggca
    71101 ctttcaaaaa gtacagtctt cgtattagaa gttatttctg agttagcacc acccattaca
    71161 ccagctaaac caagtgattt ttctccattt gttataacta gcatatcttt gtctaatgtt
    71221 ctctcaacat catctaatgt tacaagtttc tctccatctt tagcatttct tactactatt
    71281 cttccagtct ctacttgatt tatatcaaaa gcatgaagtg gttgaccaag ctctaacatt
    71341 acgaagtttg ttatatcgac tatgttactt ataggtctta ctcctgcttc tgtaagtctt
    71401 ctttgcatcc aatatggaga aggttctatt tttacatctg taaccattct agcaacatat
    71461 cttctacata aatctggatt atctatctca actttgaaat ctatctcttc gtcactttct
    71521 tttacttcga tttcaggata ttttactttt gttcctatag ttgcagctgc ttctctagct
    71581 atacccatca tgcatttaca atcaggtctg tttgaagtta attcaaaatc tattaaagca
    71641 tcttttaatc ctaaaacatc ttttatatct tttcctaatt cataagaatc ttcgtggtct
    71701 aaaatatata taccacctct tttatactcc tcaatgtaat gttcatctat acctagttca
    71761 gctgctgaac acatcatacc atctgataat tcacctctga aatcagtctg tttgatttca
    71821 actcctccag gtaacttaga accatttaca gctactggaa tataatctcc ttctgatata
    71881 tttgtagctc ctgtaactat ttgaacaact ttatctccaa tatctacttt agttacaacc
    71941 aacttatcag catttggatg ttgttttatt tctaaaatct ttccaaccaa tatattttct
    72001 atttcttcac cataataatc tattgtttca actttagttc ctgtcattgt cattttatca
    72061 gcgaactctt ttacatccat gtctatatca acatagtctc taagccattt taaagatact
    72121 aacatctaca taccctccta attaaaattg atttaagaat ctcatatcat tttcgaataa
    72181 taatctaata tcatctattt cgtatttaag cattgcaagt ctttcaaccc caactccaaa
    72241 tgcaaatcca ctgtaaactt ctgggtctat tccacaattt ctaagcacat ttggatgaac
    72301 catacctgaa cctaatattt ctatccaacc ttcatattta cacattgggc aacctttacc
    72361 accacatttg aaacaagtaa catcaacctc tgcacttggt tctgtaaatg ggaagttgtg
    72421 aggtctaaac ttagttttga tatcagaacc aaacaatttt tcaacgaaga tatccatagt
    72481 tcctttaaat tctgccatag taacatcttt tccaacaaca agcccttcta tctgatggaa
    72541 cattggtgag tgtgtagcat ctggcgagtc tgacctaaaa catctacctg gtgcaattac
    72601 ttttattggt aactcttgac ttctcatagt tcttacttga actggagatg tttgagttct
    72661 aagtaatacc ccatcattga tatagaatgt atcgctcata tctcttgatg gatggtcttt
    72721 aggagcgttt aatgcgtcaa agttgttttc aacagtctca acttctggcc cttctgctat
    72781 agagaatccc attccgataa atatatctgt tacttcatct ataatttgag ttattggatg
    72841 cttcttccca acctttaaaa cttttcctgg ttgagtaaca tctatcactt cctcttttaa
    72901 ttttctttct ttttcaatag catttatttc ttctttttta gaatttatgc taagttcaat
    72961 gttttctctt acctcattgg caaccttacc aactactggt ctttcttcag cagataattt
    73021 acccatttct ttaagtatgg cagttatctc accttttttt cctaagtact taactcttaa
    73081 actttctaca ctttctatgc tttgtgcttc ttttatttca gacaaagctg cttcacgtaa
    73141 agcaagtaat ttttcttgca cacatatcac cctttcgttt ttttgtataa aaaaagcccc
    73201 tcaatccctg tctagggacg aaaggctata gtttcgcggt accaccctag tagtttaaca
    73261 atttaaaaat catcaaacca cctctaacaa acttaacgcg tttttgacgt caaagcctac
    73321 tgtaaaattt cagctctgtt actccagagg gaacttctca tgacctctta gaaaacactc
    73381 ccagcctagg tgtttatctc tttactaagt ttaatcaaaa tactttcctc tttcattgca
    73441 tttacaatat tatattgttt attttagtat tatatcattt ccattcaaat tatgtgacta
    73501 tttttaatat atatccaata cataattaat ttaattattt tttatataat atatatctaa
    73561 tgcata
//
LOCUS       DAMJYC010000008        71771 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJYC010000008 DAMJYC010000000
VERSION     DAMJYC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.008"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            186..563
                     /locus_tag="PC472_000567"
     CDS             186..563
                     /locus_tag="PC472_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2725929.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            556..1704
                     /locus_tag="PC472_000568"
     CDS             556..1704
                     /locus_tag="PC472_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2725930.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLESIVKKKGRSGILIAILVIMTSITSFIEVDAQIKINNSINEINNNINDFILNKST
                     DKHQTFYYKDAPSKIRKFYEDNCKLKGTTPKDYDTIIINTKDFNELMK"
     gene            1721..2320
                     /locus_tag="PC472_000569"
     CDS             1721..2320
                     /locus_tag="PC472_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2725931.1"
                     /translation="MLKKSKKTLAIMCCLMLTFTSVAFADASKGEGVSKEAVSEKNTY
                     SFNISGYVYTYENAPQKIRDEHKQNCEDVNIAPKPDDKIFVPTSANLMNDDDKAVYTN
                     YAVAYKPDEKTITVIGGGEFITISTTKVIRKGDTGKEVTVAQIMLSKLGYNTSPDGIF
                     GSKTYNAVVSVQKKYGLSADGVIGPATWDKLGRLTDSSF"
     gene            2458..3571
                     /locus_tag="PC472_000570"
                     /pseudo
     CDS             2458..3571
                     /locus_tag="PC472_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3841..4863)
                     /locus_tag="PC472_000571"
     CDS             complement(3841..4863)
                     /locus_tag="PC472_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2725932.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPAIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(5192..5374)
                     /locus_tag="PC472_000572"
     CDS             complement(5192..5374)
                     /locus_tag="PC472_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725933.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            5687..6106
                     /locus_tag="PC472_000573"
     CDS             5687..6106
                     /locus_tag="PC472_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2725934.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            6237..8078
                     /locus_tag="PC472_000574"
     CDS             6237..8078
                     /locus_tag="PC472_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2725935.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            8105..9990
                     /locus_tag="PC472_000575"
                     /pseudo
     CDS             8105..9990
                     /locus_tag="PC472_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            9998..11998
                     /locus_tag="PC472_000576"
     CDS             9998..11998
                     /locus_tag="PC472_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2725936.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            12260..13519
                     /locus_tag="PC472_000577"
     CDS             12260..13519
                     /locus_tag="PC472_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2725937.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLILVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            13580..15367
                     /gene="iorA"
                     /locus_tag="PC472_000578"
     CDS             13580..15367
                     /gene="iorA"
                     /locus_tag="PC472_000578"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2725938.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVEHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            15369..15944
                     /locus_tag="PC472_000579"
     CDS             15369..15944
                     /locus_tag="PC472_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2725939.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            15997..17067
                     /gene="buk"
                     /locus_tag="PC472_000580"
     CDS             15997..17067
                     /gene="buk"
                     /locus_tag="PC472_000580"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2725940.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQCSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            17285..17986
                     /locus_tag="PC472_000581"
     CDS             17285..17986
                     /locus_tag="PC472_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725941.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            18046..18552
                     /locus_tag="PC472_000582"
     CDS             18046..18552
                     /locus_tag="PC472_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2725942.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            18727..19749
                     /locus_tag="PC472_000583"
     CDS             18727..19749
                     /locus_tag="PC472_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2725943.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(19896..20204)
                     /locus_tag="PC472_000584"
     CDS             complement(19896..20204)
                     /locus_tag="PC472_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2725944.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(20264..20692)
                     /locus_tag="PC472_000585"
     CDS             complement(20264..20692)
                     /locus_tag="PC472_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725945.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(20915..22372)
                     /locus_tag="PC472_000586"
     CDS             complement(20915..22372)
                     /locus_tag="PC472_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2725946.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            22789..23703
                     /gene="nadA"
                     /locus_tag="PC472_000587"
     CDS             22789..23703
                     /gene="nadA"
                     /locus_tag="PC472_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2725947.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            23716..25017
                     /locus_tag="PC472_000588"
     CDS             23716..25017
                     /locus_tag="PC472_000588"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2725948.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            24998..25834
                     /gene="nadC"
                     /locus_tag="PC472_000589"
     CDS             24998..25834
                     /gene="nadC"
                     /locus_tag="PC472_000589"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2725949.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            26090..26422
                     /locus_tag="PC472_000590"
     CDS             26090..26422
                     /locus_tag="PC472_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2725950.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            26584..26769
                     /locus_tag="PC472_000591"
     CDS             26584..26769
                     /locus_tag="PC472_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725951.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            26796..27881
                     /locus_tag="PC472_000592"
     CDS             26796..27881
                     /locus_tag="PC472_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2725952.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQRYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            27874..28332
                     /locus_tag="PC472_000593"
     CDS             27874..28332
                     /locus_tag="PC472_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725953.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            28367..29458
                     /locus_tag="PC472_000594"
     CDS             28367..29458
                     /locus_tag="PC472_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2725954.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            29463..29687
                     /locus_tag="PC472_000595"
     CDS             29463..29687
                     /locus_tag="PC472_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725955.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            29697..30260
                     /locus_tag="PC472_000596"
     CDS             29697..30260
                     /locus_tag="PC472_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725956.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            30221..30850
                     /locus_tag="PC472_000597"
     CDS             30221..30850
                     /locus_tag="PC472_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2725957.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            30890..31027
                     /locus_tag="PC472_000598"
     CDS             30890..31027
                     /locus_tag="PC472_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2725958.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            31027..31779
                     /locus_tag="PC472_000599"
     CDS             31027..31779
                     /locus_tag="PC472_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2725959.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            31792..32277
                     /locus_tag="PC472_000600"
     CDS             31792..32277
                     /locus_tag="PC472_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725960.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            32284..32433
                     /locus_tag="PC472_000601"
     CDS             32284..32433
                     /locus_tag="PC472_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725961.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(32569..33390)
                     /gene="yidA"
                     /locus_tag="PC472_000602"
     CDS             complement(32569..33390)
                     /gene="yidA"
                     /locus_tag="PC472_000602"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2725962.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYMQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            33883..35577
                     /locus_tag="PC472_000603"
     CDS             33883..35577
                     /locus_tag="PC472_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2725963.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      35909..36019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            36123..36494
                     /locus_tag="PC472_000604"
     CDS             36123..36494
                     /locus_tag="PC472_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2725964.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            36590..37537
                     /gene="trxB"
                     /locus_tag="PC472_000605"
     CDS             36590..37537
                     /gene="trxB"
                     /locus_tag="PC472_000605"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2725965.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            37641..37958
                     /locus_tag="PC472_000606"
     CDS             37641..37958
                     /locus_tag="PC472_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2725966.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            38227..39513
                     /locus_tag="PC472_000607"
     CDS             38227..39513
                     /locus_tag="PC472_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2725967.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            39625..40098
                     /locus_tag="PC472_000608"
     CDS             39625..40098
                     /locus_tag="PC472_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:39757..39759,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2725968.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            40130..41440
                     /gene="grdB"
                     /locus_tag="PC472_000609"
     CDS             40130..41440
                     /gene="grdB"
                     /locus_tag="PC472_000609"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:41177..41179,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2725969.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            41706..43238
                     /locus_tag="PC472_000610"
     CDS             41706..43238
                     /locus_tag="PC472_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2725970.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            43249..44394
                     /locus_tag="PC472_000611"
     CDS             43249..44394
                     /locus_tag="PC472_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2725971.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDILLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            44704..45783
                     /locus_tag="PC472_000612"
     CDS             44704..45783
                     /locus_tag="PC472_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2725972.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYAKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            45895..46494
                     /locus_tag="PC472_000613"
     CDS             45895..46494
                     /locus_tag="PC472_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725973.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(46681..47553)
                     /locus_tag="PC472_000614"
     CDS             complement(46681..47553)
                     /locus_tag="PC472_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2725974.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIDYMASIHIN"
     gene            47970..49373
                     /locus_tag="PC472_000615"
     CDS             47970..49373
                     /locus_tag="PC472_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725975.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            49400..50944
                     /locus_tag="PC472_000616"
     CDS             49400..50944
                     /locus_tag="PC472_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2725976.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            50975..52366
                     /locus_tag="PC472_000617"
     CDS             50975..52366
                     /locus_tag="PC472_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2725977.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            52559..54055
                     /gene="abfD"
                     /locus_tag="PC472_000618"
     CDS             52559..54055
                     /gene="abfD"
                     /locus_tag="PC472_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2725978.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            54190..54465
                     /locus_tag="PC472_000619"
     CDS             54190..54465
                     /locus_tag="PC472_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725979.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENIEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            54489..55796
                     /locus_tag="PC472_000620"
     CDS             54489..55796
                     /locus_tag="PC472_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2725980.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            55816..56934
                     /locus_tag="PC472_000621"
     CDS             55816..56934
                     /locus_tag="PC472_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2725981.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            57221..58141
                     /locus_tag="PC472_000622"
     CDS             57221..58141
                     /locus_tag="PC472_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2725982.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILHIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            58256..59413
                     /locus_tag="PC472_000623"
     CDS             58256..59413
                     /locus_tag="PC472_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2725983.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(59498..60997)
                     /locus_tag="PC472_000624"
     CDS             complement(59498..60997)
                     /locus_tag="PC472_000624"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2725984.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            61593..63017
                     /locus_tag="PC472_000625"
     CDS             61593..63017
                     /locus_tag="PC472_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2725985.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            63052..65181
                     /locus_tag="PC472_000626"
     CDS             63052..65181
                     /locus_tag="PC472_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2725986.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            65211..65633
                     /locus_tag="PC472_000627"
     CDS             65211..65633
                     /locus_tag="PC472_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2725987.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            65652..66803
                     /locus_tag="PC472_000628"
     CDS             65652..66803
                     /locus_tag="PC472_000628"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2725988.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      66909..67010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            67193..67765
                     /locus_tag="PC472_000629"
     CDS             67193..67765
                     /locus_tag="PC472_000629"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2725989.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            67829..68479
                     /gene="fsa"
                     /locus_tag="PC472_000630"
     CDS             67829..68479
                     /gene="fsa"
                     /locus_tag="PC472_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2725990.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            68631..71555
                     /locus_tag="PC472_000631"
     CDS             68631..71555
                     /locus_tag="PC472_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2725991.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGLGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        28347 a         7758 c        12886 g        22780 t
ORIGIN      
        1 tttaattaaa tatgtaaaaa gtaaaatatt taaatagata tattttaaaa tctaactttt
       61 aatattgtaa aaaaattaga aatttaacaa aaagtacaaa aattcacatt tttttcataa
      121 agtgtttgac taaaaaattc acatgttgta gaattaaggt agcaggagtt ttgagggagg
      181 tgtaaataat gataagaaaa ataccacaag cagaactaaa agtaatgaaa ttcatttgga
      241 aagtagatgt tacagtgaca tcaaaagatg ttattgaagc tatggaacaa aaatatggct
      301 ggaaacaaac caccacactg acacttttat ctaggctggt aaaaagagga ttcttagatg
      361 ctcaaaaaat agacagatat acacattaca caataagagt aaaacacaaa gattatttaa
      421 gttttgagac aaaggatttc ctaagtaata tacatgatga ctctttgaga agtttaattt
      481 cagcattaca tgatgatgaa aatataggta aagataagct agattttcta gaaagttatt
      541 ttgataattt aaaggatgaa taattatatg ctatatgttt ttgaaaatct tataaagaca
      601 actattgtat gtagtcttgg tatttgtcta ttattatttt tgaaaagata tttatttaag
      661 aagtttagta agagattcaa ttattatatt tggttgataa tagtctttag aatgttactt
      721 tttctattta attatacaat agtatatgaa gtaaaagaac ccaaagaaaa tgcattagga
      781 aataatatta cacaaattga tatttctaca gataataatc ttatgctgta tgtagcttat
      841 ttatggttat ttgtaactat agtcattgca gtttatacat ttataaagta tacaagattt
      901 aaaaatctag ttgttgatgt atcttatgat attgaagata atgatataaa ctgtatctac
      961 aaaaacttgt taaaagaatt aaatataaaa aagaaaattg aattaagagg ttctgatgaa
     1021 ttgataagtc cagcaggcat gggattattt aaatcttaca tattcttacc agactatcct
     1081 tatagtaaag atgaactaac ttggattttg aaacatgaat taatgcattt taaaaataaa
     1141 gatattttaa taaagttttt agttttgtct gttagaataa tatattggtt taatccactt
     1201 gtatatgtaa tgagcaataa agtaaatcta gattgtgaat tatgttgtga tgaaagtgtt
     1261 ttgactgatt gttctttaaa agataagaag gagtatgcat tagcacttat aaagtcaatt
     1321 aaacttagta agaattataa tagtggtatt ttaacaactg aatttaataa aacaaattta
     1381 gaaaagagac ttgagagtat tgttaagaag aaaggtagaa gtggaatatt aattgctata
     1441 ttagtaatta tgacttctat aacatctttt attgaagttg atgctcagat taaaattaat
     1501 aatagtatca atgaaataaa taacaatatt aatgacttta tactgaataa gtcaactgat
     1561 aaacatcaaa ctttttatta taaagatgca ccaagtaaaa ttagaaaatt ttatgaagat
     1621 aactgtaaat tgaaaggtac aacacctaaa gattatgata caataataat taatactaaa
     1681 gatttcaatg aattaatgaa ataattataa ggaggatttt atgttaaaaa aatcaaaaaa
     1741 gacattagct attatgtgtt gcttaatgct tacttttaca agtgtagcat ttgctgacgc
     1801 aagtaaagga gaaggagtga gtaaagaagc tgtttcagag aaaaacacat attcttttaa
     1861 tatttctggg tatgtatata catatgaaaa tgctccacaa aaaataagag atgaacataa
     1921 acagaattgt gaagatgtaa atatcgcacc taagccagat gataaaatct ttgttccaac
     1981 atcagctaat ctaatgaatg atgatgataa agctgtttat acaaattatg ctgttgctta
     2041 taaacctgat gagaaaacca taacagttat aggtggtggt gaatttataa ctataagtac
     2101 aacaaaagtt atacgtaagg gtgatactgg aaaagaagtt acagttgcgc aaattatgct
     2161 atctaaacta ggttacaata caagtccaga tggtattttt ggaagtaaga catataatgc
     2221 ggtcgtaagt gtacaaaaaa aatatggttt atctgcagat ggagtgattg gtccagctac
     2281 ttgggataaa ttgggtagat taactgattc tagtttttaa taggattaaa atctaatatt
     2341 aaatagtgtt ccaatgtgat ttcttaagag cttttaaaat aaagtgtgta ttcttctaaa
     2401 aagtgatgat aattaaaata taaaatatca ctatacaaag gaaggtacac actttttatg
     2461 tttaacaaaa acgaaatatt aaaaaactta ttgaagaata tgatgtcaaa acaactacaa
     2521 atattcaaga catactaaag gttttatttg caagtacaat tcgtcaaatg ttggaaacgg
     2581 agtttgatga ctatttaggt tatgataata agaataaaac tgctaaaaac tctagaaata
     2641 gatatagaag taaaaatgtt gaattagatt ttggagaaat aactttaaat attccaagag
     2701 actgaaattg agattttgaa ccaaatgtaa ttagaaacta tgaaagtgat atatctgaaa
     2761 tagagaacca agtgattgga atatactcaa aaaaggtatg tctactcaag atatatcaag
     2821 gcacattaaa agtatttatg gaatggacat atataattaa ttaatagcta aagctactga
     2881 tatagttata tctttagctc atgaatagca aagtagactt cctgatatga tttattcaat
     2941 tgtatttatg atactatata gtataaagtt ataagttaaa atagaataaa taaaaaagca
     3001 gcatctatag ctacaggtat taacttaaaa gggataaatg aggttttatg aaccatgggt
     3061 tggagaaatg aaagtttaaa atgttgccta aaggtactta cagatattag aaacagagga
     3121 gttaaagata tattaatagc ttctgtagat ggattagtta gattttctga tgcaattaaa
     3181 gcagtatttc ctgatactga aatacaaaga tgtgttgtgc atcaaatcag aaatacttta
     3241 aattatgtat catataaaaa tagaaaaaaa tttgcaagaa atttaaagaa aatataggat
     3301 tcaattatag aagaataagc gttaagtaag ctattgctct taaaagaaaa aaggggcaat
     3361 caatatgcga tagcattaag aagttggtga atcagttgaa atttacaata gattattaag
     3421 aaaagttact aaatcgaagt ctatttttcc ttcagatgat tctttgcaca aatcattata
     3481 cttagcaact atatatattt ctgaaaaatg gactcaaatt ttatcttatt taagtattta
     3541 ttttaaagaa agaaatttaa atctaaatta ataagaaata tttcaaaaaa tcaagaattt
     3601 ttcttaaagt gataataatt tttgcaaaaa tggaaaaaat atcaaaataa ggctatgatt
     3661 tttaaagcta tagccttatc agtaaaaatt taattatcat ttaaaaaata aatttttatt
     3721 taaagaatag acataaatat ttccagactc tctcaatatg atttattaaa cactaaatga
     3781 gatactattt tgtatacgtt aaaggatata ttatttaaat ataaatgtta taaaactatt
     3841 ttacaataaa ttccatataa ctctaccatc tatatcctta acattttggc aaccagtcaa
     3901 aatcatagtc tcacataact cattcctaac cttctctata aaaaactcca caccatcaag
     3961 accaccgcca aaagaagcag taacaaaagg tcttcccatc aaaacagcat cagcacccaa
     4021 tccaagcatc ttaactacat caacaccagt cctaacgcca ccatcaacaa gtattgttat
     4081 ctttcccttt acagacttag ctatagctgg aagtacctca caagtaccag gagtataatc
     4141 aagtactctt ccaccatgat ttgaaacaat aatggcacta gcaccagact ctacagccat
     4201 caaagcatca tctacagtca taattccttt tataataaaa ggtaatctag tggattcaac
     4261 caattctcta atctcatcaa ttgttttagg agaaaaagga ttttcttgaa attgattatt
     4321 aataagtcca caagcatcaa ggtctacacc aactgcaaaa gcacctgctt cttcagacaa
     4381 tctaattttt tctattatct ttgaattatt ccaaggcttt ataaatacaa ttccatttcc
     4441 tctattatcc ttcaacacat ctaaattatc cagtaaaaaa gtatctacat tagtatcgcc
     4501 aaccatagca taaattccag aattagaaca acctctaaca acaggctcta tgtattcttt
     4561 ttcggaaact ttaccaccca tgttcaatat agtacctgat acaggtgcag cgaatatagg
     4621 agaactcatt tttctaccaa ataattctat ggatgtatct ggctttgaaa cattatgtat
     4681 gactctcata tttatcttta ttttttctag actttttcta ttttcaatga aagacgaacc
     4741 acttccttta ccacccatac caggaacatc tccagcacaa gctaatccat tacaaatttt
     4801 acatacttta catgttccat taaagttctc tctagctgat tttagtaagt cattgtaatt
     4861 catacaaaat ctctcctttt tataatatta aaattgagta ttactatatt gaaaatatct
     4921 taattgtaaa tatagggatg ttattatgat gagctaaact agataataca tattagatat
     4981 tttttattgt tgtagatagt taaattatat catattagat agtcaaattt tgaaaaaaac
     5041 taacttattt aagtatacat tttgaaaaaa ttaaaaaagc tatatataga tttttaaaga
     5101 aatctatata tagcttttaa gtttatgtca atcttttttg ttaatgaata aataatatag
     5161 aatacaatat acaaaaaagt ttcttgaaat gctaagataa tccccattta caaagcagca
     5221 tcattatact gtgtaaattt gaatcaacaa tgttttcacc atatttcatc ataaaatcct
     5281 ctaattgtgt gtcaataaat gaataatatc cacttatact atcgatgtat gcagtcatta
     5341 agtgtataca ttcatcactt ttatctttat acatatatat ctccccctat atacaatata
     5401 taaatttaga taccaaatcg tatcatatta aatagttaag tgtgtaacat ctatgcagtt
     5461 atatatttat atcattatat taataactaa agtgagtttt gacaatagat tttgggaaac
     5521 tggatgttca aagtgcaaac ttggatttta aaatcattaa ttaagatagc gcataggaat
     5581 atatgtatac ataacttaat tataaagatt ataaacaatt acttttatta actttattta
     5641 aatggtagaa taacttggag attaataatt ataaggagga tataatatga gtttatataa
     5701 gtttatatat gatgataagg aatatttatt aaaagaggag aactgtagtg ctttaataaa
     5761 tgatgaagaa aatccagttc aaggagtaag tatttcaaaa ataattgata tacttaatga
     5821 agcagaagaa gttgattttg atgtagaata ttatcaagaa gcatgtccat tatgtttaga
     5881 aggagtaaaa gaaaagaaaa aattctttcc ttttttagaa tatcactttt atattttcag
     5941 taaagatgga gaatatgtaa taagcaatat atctgttgat tataaaggac tatcttttaa
     6001 taaactatca agggcaaata aagttgataa tagttatatt gtaagtgtga taatttgcga
     6061 aaattgtcag gattatatag ttcagataga aaattgtata gtttagtgta aagtatgtct
     6121 taatttaaga gaaatttcta aattgagaca tcaacttttt tcaaaaatag tatggaaaat
     6181 tactaaattt atgagataat gtaagtcgga ggtgagttac aatatatagt aaatatttgg
     6241 aagagataga tgatacaatt ttagattcaa tgtttaaact aaccttgaat gaaagattat
     6301 tgactaataa cgattttatt gaaaataaaa ttgatgtagt aaagaattta attgattgct
     6361 ataaaactga acaaaatagt ttaatgttat taaaatcaaa ttttttgcta gctttattat
     6421 atggtattca agggtttagt gaaaaaatga aagagtatat tttgatttcc tgtaaatata
     6481 ttgataaatg cttacccaaa gattataaat ttttagcacg attttattca caaatagcta
     6541 ttttaagttt aaaaaatgaa gatgtaaata aggcaaattt atatatggat aaatttaatc
     6601 ttatttgtga tgaaaataat tttctaattg aagaaattat ttttaaatca caacttattt
     6661 acattaaggc aagtaagtgt atagaatctt ctgtaataat taaagaaata gaaacgttgt
     6721 atataaagat aaaagaaatt aacgatttta attgtaaaaa aatatatttt tttattattg
     6781 gtaaaatata ttttttgttt ttggatgatg ctctaattgc taaggtaaat tttttaaaag
     6841 caagaaaata tgcagattta ctaaaggata tgcaagtatc ttcactatgt aatataaaga
     6901 taggtgagtg tgaatgttcg tttgaaaatt atgaatgttc catggaatat ttcaatgaag
     6961 taataagcaa taaaaagtat agaaatgtaa atataattca aaaatataga gcatcaaaca
     7021 acataactaa aatattaata aagactagaa attatcctgt cgcaataaat aatttagcaa
     7081 aatctgaagt atatctagaa aaaattaaaa atcatagctt aaaagaagta gagaagtttg
     7141 atttatttat aagtttagcc atgtattatg caaaaagtaa tgaaaaatct tttgaacaat
     7201 caatatgcta tttagataga gctaaaaaca tattaaatat gataaaaaat atagaagaat
     7261 atataatata tgatttagaa ataatttatc accaggcaaa gatatattat atctttggat
     7321 attatgacaa tgccttaaac acaaataaaa aactccttga aaaatctaaa aatgctaaaa
     7381 attataatta tgtgaagtta gcatacaaga gtatatattt gtgttttgaa aagattcaaa
     7441 attatgaaat gagtatgaaa tactttaaaa tgtattatga gttaaaaatg atgtatataa
     7501 aagctcgaaa tagaaattat atagattcct taaattatag acatgagtat atagaaaaag
     7561 aaataaataa catgagaaaa gtaaaacttg atttagataa aaaaaagtat gttgaccatt
     7621 tgacaagtgc atataataga acttttttga acgatttttt agaaaaacaa gaagttagtg
     7681 aatattgcac agcatttatg atagatgtag attactttaa agaatacaat gatacttatg
     7741 gacactataa tggagatatt gctcttaaaa atataactat aataataaaa aaatatctaa
     7801 aaaaagacat gcttatgata aggtatggtg gagaagaatt tctgatatta tctgtatgta
     7861 aagattataa aaaaagtaaa atatttggaa gaaaattatg taggattgta aagaaatttt
     7921 taaacaataa tttgacagtt agtattggca ttgatacatg taaaaatagt agtatagata
     7981 taaaagaaat aattgaaaat gctgataaag ctttatataa agcaaaggaa agtggtagaa
     8041 atagatgttt gcattatcat gattttaaga atttttaact gttgaaatag gattggtgat
     8101 ttacttgaaa aggagtttaa tagaagattt aaagagaaat ataaacaaga tagaatacga
     8161 tatgttgact aaattctatc taaatttgac agatttaaac tacatcttgg attatcacaa
     8221 agacctttta aatacaaaat tagaagataa tagtagagtc tatactttat atttaattag
     8281 tcttataaac tatataaaaa aagatagaga agcatcatac aactatttga tagagtcatt
     8341 aaagtatatt aaaaaattgt cattaaaaga tgattcagat ataatatcta aaatttatat
     8401 ttatcttact ttatcagcta tttctataag aaaatatgat aaggtaaata atttttattt
     8461 ctcagcaaaa aaattataag acaaaatcat ttaaatgagt tgttggtatt gttaaatatg
     8521 agtttatgtg tagaaataag tgctttatat cagaccaaaa cggatgtaat agctttagta
     8581 gaagaagtgt ctttttttaa aactttaaaa aataaagctc ttgtaagtag ggcaaattat
     8641 atatttggga gagtttatct atatttattt aataacttta taaaaagtat ggaatactta
     8701 attgaatgtc taaaattggc tcaagaaaat aatttatact ctatagaggc tttttgtaaa
     8761 tgtataataa gtttgtgtta tttagagagt ggaaataatg tagaagctat aaaatacata
     8821 aataatgtat tagatgttgc acacagtaat aactcaatat cagtaactga aagagtgtct
     8881 ataaagatag accttatatg ggcatattta aaagaagata tgaataaaga agcagaagtt
     8941 attttattaa agactataaa attaatgaaa gttgttgaag gtgtatataa ggattattta
     9001 tattcattta tatttttata ttcagctgaa attgagttaa agaaagataa ttgtagtttt
     9061 gaaaaggtaa acacctattt aattttatca aaaaatatat ataaaagttt tggagatgtt
     9121 tttatatata attctaaact agattacata tataagttgt atggagattt atatataaaa
     9181 tttggtaata tagatgaagg tataagttgt tatataaaag gtttaaattt agttaaaaag
     9241 agttcattga atcttagaaa aatatctata ttttatgggc taatagcaaa tggatatgag
     9301 ctaaaaaaag attttaaact ggcaatagaa tattataaaa gtatggattg gtattctgat
     9361 aggtgggaaa aaaataattc ctataaaaca tctcaagcaa ttcacaaaca atatgaactc
     9421 agacaaaaac aagagagttt aagattacta gtggatgata ataagaaatt agaaaatgat
     9481 attttaaaag atggacttac taaactatat aatagaagat atttggaaca gcagttggat
     9541 ttatttaatg gaaccagaga caaaaatatg gtcgcaatac tggtcgatat agattgtttt
     9601 aaaagttata atgataatta tggtcatcta gcaggagata aagttatagt taaggtaact
     9661 gatattataa gaagtgtatt tatagatatt accgaacaca taatcagata tgggggagaa
     9721 gaaattttaa tattagtaga ttctatagaa tcaatcggtg ttgaagagat tgataatata
     9781 gaattatata ttaaaagagt tttcaaactt cttgaatttg agggtattga acatatatat
     9841 tctaaaataa aaaactatat aactataagt gctggagtaa gtataaaaag gtgtcgtagt
     9901 agaaaagatg ttgaaatatt aattgaagat gcagataaga acctatataa atcaaaaaaa
     9961 gcaggtagaa atcaatacat tataatttaa aattgaggtg attttgttgc gaaaaaaaat
    10021 aggaattata gcctctgata ttgaattaaa agaaagaata gaagaattat atagagaaga
    10081 tgtagaaaat ggaactataa taatcgacat acttaaccta gacttaatgg aaaatcaagg
    10141 tcgtatactt gttgaaaaag gtgcccaagc tataattggt agaggtggag gttatagctt
    10201 agtaatagat acagtaaatg tacctgttat accaatgaat atgaagtcaa ctgacctttt
    10261 aagagcaata gaaatagcaa agaaatattc taaaaaagta gtattgattc ttggagacaa
    10321 tgaagtttct tttgactatg ttggctggag aaatgtcata agtactgaaa taactgaaga
    10381 atggtttgaa tctaagtatg agataagaag taaagttgta aaatatatag accaaaaaga
    10441 tgaagttgta atcgtaggag gaggtttggc atgttctttt gcaaggcaat atggaattga
    10501 tagtgtattt gcaactgcta gtgatgaatc tataagagaa gctgtagaat attgtaaaaa
    10561 attattagat acattaggtg aagaaaagtt taataatgaa gtgttacgaa atatacttga
    10621 tggtataaaa gatggagtaa ttgctataga tagtaatggc agtataattt tgtacaatga
    10681 aagtgcaaaa aacatgctta aagtagagag aaaatgtgca cttaataaat acatcttaga
    10741 tgtatttcct aaaatggagt ggatgttaga ttgtttacat gaaaaagagg acgtagaaga
    10801 tagaaagata agaaatataa ataatctaat tgttaatact aggacaacat tgataaaagt
    10861 tgataatagt acttatggag ttttaggaat aatacaagat ataaccaagc tacaaaactt
    10921 agaaagaaaa ataagatttg acttgaatca aaaaggtctc tatgctagat atacttttga
    10981 tgatttttta tttaaagata aattaactaa agaatttatt gaagaagcta aaaaaattgg
    11041 aaaaagtgat tatacaactt tactttatgg agaaagtggg tctggtaaag aaattatagc
    11101 tcatagtata cacaacataa gtaaaagaaa agataggcct tttgttgcta taaattgtgc
    11161 tactatagca gaaaatttac ttgaaagtga actttttggc tatgaagaag gtgcatttac
    11221 tggtgctaga aagggtggaa aaagagggct atttgaactt gctcatggag ggactctttt
    11281 tttagatgaa ataaatagtc tatcctttaa tattcagaca aaactattaa gagtaataga
    11341 ggaacgtcaa ataatgagaa ttggttctga ttatataata cctctggata taaggattat
    11401 agcagcaacg aacgaatctt taaccgaaaa aatagttatg ggtacattta gagcagactt
    11461 attttataga ttgagtagtt tagagataaa tatacctcct ttaagagaca gaagagaaga
    11521 tataatacct ttgtttaaca attttgtaaa tgaagtatta aaggatgatg gtctcaatgg
    11581 aataaacagt atagatgaaa attttgtttt gactaaagat gaaatggata agctatataa
    11641 ttatagctgg cctggaaatg taagagagtt aaagacaata gctcaaaaat atgttgtgac
    11701 aggtaaaatt aaattgagac aagatagaga ttttaaaact aaaaaattac ttcctaactc
    11761 tgaagtagac aaatttaatt ctgaaacgac agcaagtgta gaagtccaag atgaatctat
    11821 aaatataagt aagataaatg atggtaagat aagtatagat ataaaagaag taaataagta
    11881 tgttgaagaa aaaataataa gtatgctttt tgctcaagga ctttctaaaa atgaagttgc
    11941 acaagttctt ggaataagca ggacatcttt atggaagaaa tacaataaaa atatttaaat
    12001 ataattacat ataaattatt ttgaagtaat gttaattttt taaaacaagt gttaagaaaa
    12061 attaacatta cttttttgca aatttttatt tttttagaca ggcatatata tttataagaa
    12121 aaatagttaa aacacttaat ttaataatga aaaaaataat aattctaaac aaaatcaatt
    12181 ttacataaag tgggatttat tggcatagta attgcttaat atataatgta agagtaaata
    12241 caattttagg ggtgatggta tgagtcaaag tatggttggt aagcatgcaa tgtggccaaa
    12301 agaaaatgat gttatattta gcatatctgg cagagctcaa gcagcagaga aggcttttgg
    12361 tatggataat gtaataaatg caacaatagg cgctctaatg gatgattcag gaaaattaat
    12421 aacaatgaaa actgtatatg aagaatataa agcattagat aactgtgaga taggtgcata
    12481 tgcagctctt gaaggtcaac cagattactt agaagcagtt aaaaaagtat ttttcagaga
    12541 ttacttacca gaaggacata taagagtatt agcttcacca ggaggaagtg gtgcaataaa
    12601 attagctgta tggaactata ctaatgaagg agatgaggta ttaacatctg attggttctg
    12661 gagtccatat gtaagtatag ctgaagaggc taatagaaaa gtagttaatt accaactttt
    12721 tgatgaaaat agaaggttta attttgagtc gtttaaagaa aaatttgtaa atatagcaga
    12781 aaaacaaggt agagtcttta caattataaa cacaccagcg cataatccaa ctggatatag
    12841 tgttgcagat gatgagtggg ataaaatatt agacttatca aaagaagtcg caaaagataa
    12901 agacaaaaag ataatatttt tcgtagattc agcatatatt gactttgcag gtgatgatga
    12961 tgtatgtaga aagtttttta agaaattttc taatttgcct gaaaacgttc tcatattggt
    13021 tggattcagt atgtcaaaag gatttacagc ttatggtatg agaatggggg ctataatttg
    13081 tatatcttca agtgaagatg tagctgaaga gttccattac tcatgtgttc attcatgtag
    13141 agcaaattgg tcaaattgta atagaagtgc catggcagta ttatctaaca ttgtaaatga
    13201 ccctaaaaaa ttcaaagaat atgaggatga aaaagaaata tacaaaaaca tgcttactag
    13261 aagagcagat gtatttgtaa aagaagctga aagagtagga ttagaaattc ttccatatat
    13321 agcaggtttc tttgtaagta taccatgtga taatccaaaa gaagtatgtg aagaattgac
    13381 taaacataat ttatttgcag ttccattaaa aatgggctta agatttgcag tttgtgcagt
    13441 ttctgaagat aaatgtaaga aagcaccaag tattataaaa gaagcacttg aaagtttaga
    13501 agtaaaaata aataattaat ttcattgaac aaattgggtg gactatgaat ttaataattg
    13561 tagatggagg gaattttata tgaaacaatt gatgacaggt aatgaagcaa ttgcacgtgg
    13621 agcctatgag gctggtgtta aatatgcatc tgcatatcca gggactccaa gtacagaaat
    13681 attagaaaat atagcaacat ataaagatgc aatagttgca gagtgggctc caaacgaaaa
    13741 ggtagcactt gaggctgcta tagggggttc aattgcagga gcaagaacaa tggcttctat
    13801 gaaacatgtt ggtttgaatg ttgcagctga tccaatattt acttatgcat atacaggggt
    13861 aaatggtggt atggtactta ttacagcaga tgagccggga atgcattcat ctcaaaatga
    13921 gcaagacaat aggatgtatg caaaatttgc aaagatacct ttatttgaac catcaactag
    13981 tcaagaagct aaagatatga taaaagaagc ttttgaagtt agtgaaaaat acgatacacc
    14041 agttttatat agagttacaa caagactttg tcattcaaaa ggattagtgg agtgctatga
    14101 tagagaagaa gttgaaatta aagagtatgt taaaaatgct aagaaaatgg taactgttcc
    14161 agcaaatgct caaataagaa ggggcgttgt tgaagaaaga atggaaatcc ttaaaaaatt
    14221 ctcaaatgaa actgatttaa actactatga aataaatgat acaaaaatag gtgttatagc
    14281 ctctggtatg tgttgtaatt ttgccaaaga agtttttgga aaaaatgcat catatatgaa
    14341 actaggattt acaaatccac taccatatga aaaaataaaa gaatttgcag aaaaagttga
    14401 taaaatatat gttatagaag aaaatgaccc atttatagaa gaacaaataa aagcatatgg
    14461 aatagattgt attggaaaag atgtaatacc accatatgga gaaatgacac cagatgtttt
    14521 aagaaaagct atatttggaa aaacaaatga tactatagaa tacaaatctg aattagtaac
    14581 accaagacca ccaagtttct gtgcaggatg tccacataga ggattcttct atgaacttgg
    14641 aaagagaaaa aatttaatag ttggtggaga tataggttgt tacactcttg gatttgctcc
    14701 tccatataat ggaatagact atgttgtttg tatgggttct gcttttggaa ctgcacatgg
    14761 agctcaaaaa gttcttaata tgaaggatga taatgaaaaa agattggtag gtgtacttgg
    14821 agactcaaca ttcttccata caggaataaa tggactttta gatgttgttt acaatagagg
    14881 taattctatt tcggttatat tagataatag aataactgga atgacaggtc accaagaaaa
    14941 cccaggttca ggatatactt tacaaggtgc taaaactaaa gaagttgata tagaaggatt
    15001 agttaaagct tgtggaatag aacatgtaag agttataaat cctaacaatt taaaagaagt
    15061 aaatgaagca ctagattggg ctttagctat agaagatgaa ccatcagtaa taataacaag
    15121 atggccatgt gtacttaaaa aattctctaa agaagatata gaagaattta ataatccatt
    15181 taagactaag tgtaaagttg agcatgataa atgtatagga tgtaaattat gtttgaaaac
    15241 tggatgtcca gctctatcat ttgataaaga gaataagtta tcaaatatag atagaaatca
    15301 gtgtgtagga tgtggagtat gtgcacaagt ttgtccaaaa caagcaatag ttaaggagga
    15361 aaaataatat gactaagagt atacttttgg taggtgtagg aggtcaagga actatacttg
    15421 ctagtaaatt attaactatg ggattgatgg aagctggata tgatgtaaag atgagtgaaa
    15481 tacatggaat gagtcaaaga ggaggttcag tttcctcaca agttagatat ggagattgtg
    15541 ttcattctcc agttatagaa attggtggag cagatatatt agtatctttt gaaaaaatgg
    15601 aagctttaag atggttcaat tatttaaaac cagaaggaaa agctttagta aataatcata
    15661 ggatagattc tatgactgtt ttaatcggag gagctgaata tcaagaaagt gatatagatg
    15721 cagagttaag tagattaaat gcaaaagtta taaatgcagc tgataaagct gaagaactag
    15781 gaaattcaaa aataatgaat gttattttat taggatgtct agttaaatct atgcaattag
    15841 agtctattga ttgggaaaaa attataagtg aaaatgttaa accaaaattt gttgaattaa
    15901 acataaaagc tttccatgaa ggtatggaaa tggttagcaa atagtttata atagatataa
    15961 atagactata gaaattaagg tgaaaggaag aaaattatga cttacagaat attagccata
    16021 aatccaggtt ctacttctac aaaaatagca gtatatgatg gagaagaaca aattcttgtg
    16081 aagacgatag accatccagc tgaagagatt gcaaaatata atactataca agaccagttt
    16141 gaaatgcgta aggaagcagt tttgaatatt cttaaagaaa atagtataga cttaaaatct
    16201 cttagtgcaa tagtaggaag aggtggagtt ttaccaccag taaaatcagg agcatattta
    16261 gtaaatgaag aaatgattga tgtactaaga catagaccag tacttgaaca cgcttccaat
    16321 ttaggtgctg ttgtggcaca tgcaatatca gaacctcttg gaatcaactc atatatttat
    16381 gattctgttg cagtagatga gcttatagat gtagcgagaa tatctggact ttgtggaatg
    16441 gatagatcaa gtgcagggca tgcattaaat actagagcaa tggctttaaa atatgctaag
    16501 gataaaggaa aagattataa gagcttaaac ttaatagtag ctcacattgg tggaggagta
    16561 agtatttatc ttcatgaaaa aggaagaatg gttgatatgc tatctgatga tgaaggacca
    16621 ttttctccag aaaggtcagg aagagtacct gctacaaaat tagtggctgc ctgttattca
    16681 ggtcaatgtt cagaaagaga aatgactaaa aagataagag gtaaaggtgg tatagtttca
    16741 tacctaaata ctgtagatgc tagagaagtt gaaaaaatga tagcagaagg aaatgaagaa
    16801 gcaaaaatta tttatgaagc aatggcttat cagttagcaa aaggtattgg agagttagca
    16861 actgtagtag atggaaaggt agatgctata attataacag gtggaattgc atattctgaa
    16921 atgtttactt caatggttaa aaagaaagtt gagtttatag caccagtaga aattatggca
    16981 ggagaaaatg agttggaatc acttgctttt ggaactttaa gagtactaaa tggagaagaa
    17041 gaagctagaa tttatagtga aaattaataa gaaaaactaa aaatgtttat atactaaaaa
    17101 agtggtatat ccgtttttta tacacgaaag cttagagtat aggtgaaaat gtataaaatt
    17161 tggattgcca ctttttataa ttaaaatttt atcttaacct aataaataat tctgtaaaat
    17221 ttcgtattta tatatatatt ttattggtat ttattgttat tattattatg aggagtgatt
    17281 tttgatggga aaaataagtt taaaaagtat gataagatta ttttcaggtt tttttatatt
    17341 tgcaatttct tctgtgctta tgattaatgc acatgtaggt cttatgcctt gggatgtact
    17401 tcaccaaggt ttatctataa agctaggcat aactataggt caggcttcta taatggtagg
    17461 agttgtaatt gttatattgg atgcagtttt tggtgaaaat ataggatggg gtactttact
    17521 caacatgaca tttataggaa tttttattga tttagtaata ttttctggag tgatacctca
    17581 tgcaagcaac acttatatag gtgtgtttat ggttgttata ggaattattt tggcagctat
    17641 agcaagtttt ctatacttag gtgtttgttt aggaagtgga ccgagagatg ggcttatgat
    17701 tgctcttcag aaaaaaacta ataaatctgt acgtcttgtt agaacaattc ttgaaatact
    17761 agcattagtt gttgggtggt tattaggagg ttctgtaggt attggtacgc ttgtaagtgc
    17821 tttaggttta ggatatgtat tacaaatagt gtttagaata tttaaatttg acacaaaatt
    17881 gttgaaacat agatttatta tagatgatat aagagaatgg aaggaaaaga aaagcaatga
    17941 acataaatgt aaatcaagta ttgtaataaa aaatgagcag aattaattat gctaaaatat
    18001 atttgtagtt tatctaattt atagtaaagt caaggagatt ttgaaatgga attatgggat
    18061 ttatataatg ctgatggaat taaaacagga aatgttataa aaagaggtaa ttctattgaa
    18121 gaaggttatt accatttggc agttgaagtg tggattttaa atagtaactc tcaaatttta
    18181 attcagaaaa ggtctaaaag taaaaagaca ttgccaaata tgtggggaat gacaactgga
    18241 tgtattgttt ctggggaaga cagtttagaa ggagcaattc gcgaagcaaa ggaagaaata
    18301 gggatagata taactaaaga tgagatgaaa gtttttagaa gtatgattca tgaagatact
    18361 ctttgggatg tatacttagt taaaaaagaa tatgatatat ccaaagctat tttacaggag
    18421 gaagaagtaa gtgatataaa gtgggtaagc acagatgaaa taagacaatt actaaaagag
    18481 ggtttgtttt ttgaatatcc agagatatac gaattgctat atgagataga taataataaa
    18541 ttaaatatat gatgtagagc agatttcagt ataagtaaga ttatttaaaa tgtatcttct
    18601 ctaaatttta atattgtttt atgaaaatta ataagttatt tttagtattt ttctacttgt
    18661 tttttgagat taaaataaaa agggcagtca ttataaagtc ataaaaattt ggggaggatt
    18721 taaactatgg aagataacaa aggattggca cgtgacagtt caaaatataa aattgtttct
    18781 atatctgtcg tagtaattat tctaattaca ttttggtata tgttaaatat ggtgctttta
    18841 acatttatta tgacttttat tttctataac ttgcttgtag ccacaagaaa acgaattaag
    18901 aagttttctt ctttaaatat tccagattca ttgataatta tagtgttata tgcactattt
    18961 gctatattgt tagtattgat tagctatgca gttgtaccaa taattatagt tcaacttaca
    19021 gagttgagta gggtttttag tgattttgat gtaaatcaat ttgctcaatc tttaggacca
    19081 aaattgtatc ctattgtctc aaagctggat tttaataaat atatttcaca agcagggcta
    19141 cttatagcat caacagcaac aaaagttgga agttttggag taaatatatt attggcattt
    19201 ttactgagtt tactcctttt acttgaaaaa aatgaaatta aaaattttgg agataaactt
    19261 tctgatagta agatttcttt tatttataat tcgcttgtat tttttggaaa gagctttgtt
    19321 aaaaattttg gtgaagtaat gaaagtacaa gttatgattg cctttataaa ttctgttgtt
    19381 tctatgattt ttttaggttt catgggattc cctcaaatat gggctttagg atttatgatt
    19441 tttgtgttgg gtcttattcc agtagcagga gttatagtgt ctttgattcc attaactgta
    19501 atagcattta atacaggagg aataacaaag gtttttggag ttttacttat gatttgtata
    19561 gtgcatgcag tagaaacata catattaaac cctaagttga tgtcgaatag aactaaactt
    19621 ccagtttgtt ttgtttttat tattttactt gtaggagaac attatttagg tgtatgggga
    19681 ttactaattg gagttccaat atttatgttc cttatggata ttctgggtgt taaatttact
    19741 tctaggtaat atattaaaat aataatttat ctttatatat aaattataaa ttgttttaaa
    19801 aaagaaccta taaatagaca tcattaataa agacatctat ttgataggtt caattttgct
    19861 ttgtataaat actataaaac taaataaaaa gaaatttatt tcatttcaaa agtttcacaa
    19921 cttgcatttc tttcatttat atgaactttt tcagctttgc aaagttcatt tttattgtat
    19981 ttacatttta cagcttcaca atgaatgtta tctgtatcaa cttggttagt ttcattagaa
    20041 cgactagtta taccagatgt atatttgtct tcaaaagaag cacaataagt attttctgta
    20101 gtagttgctt gcattccatc tacttttata ctaccagcat aacaatatcc actagtatta
    20161 taagcacaat tagtagcagc acaatttaaa ttattatttt tcataaataa cctcctaagt
    20221 tttatttaaa cttattatgt gaagttatct aaattttata cacttattta caattacatt
    20281 tatcacattt aacaactaca tctttggaag tttcccttcc taaattttta ttaacatgat
    20341 acacatcaac attttcagtt gatccacata tagaacattt aaaattgttt tctagtaggg
    20401 ttctttcttt aaataacttc caatacatag aattgatgta atcattataa tttaccttga
    20461 ggcatgtctc aagaaagttt atgtatagat tgtctaaatc tcctgtattt aattctgtat
    20521 attctggttc aaaatattca caacatcctc taaaatcggg ctttgtatct gaaataacac
    20581 atctaatatc atatctatag tctaggctag gccccttaaa tgaaagatgt gtgcaaaaag
    20641 aacaagatgt ttctggtaga tttttttcaa gttcatgtaa tttattattc ataagtattc
    20701 ctccaaaaaa tttattttaa gtatataaac gaatagatta ttttaaaact tttatttatt
    20761 tatattagag atttaggata aatattccaa taaggagtaa atacttaagt atttattact
    20821 gtattaaata aaaaagaggc aaatctaact tgagattgcc tactttttta tcatttttta
    20881 ataaaattta ttctaagtat tattttattt agatttagtc agccaattta gtatctgctt
    20941 tgctttttaa ctttctacca gagcgtacag ttaaaatagc atataataca gtaaatatgg
    21001 cagctaaaac atatgatata tttatgttta agttaaatcc aatttgagca ttaaatatat
    21061 aactgaatat aacaaaggaa taaaacatac caggaattaa gcttataata taattctttt
    21121 cttttattat aaggtaaact gtaatcattg caaatgcaaa tatggcaata gtttgattac
    21181 tccaagagaa gtatctccac aatatattaa atccagaagc actaagcttt gcaaatataa
    21241 ggatggctat gacaggaata aatatacaga ttgacaatat aacacgatgt ttcttttcct
    21301 tttggtcata atgaaagtaa tctgcaagca ttaatcttaa agatcttaaa gctgtatctc
    21361 cagaagttat tggtaaaaca attacaccta ttatagctat gataccacca atagaaccaa
    21421 gtaaatctct tgcaaccaaa cctattacat caggagaacc tactaattcc ttaggtattc
    21481 ctttattgta aatacccata gcagctgcag cccaaatcat tgcaattaaa ccttctaaaa
    21541 tcatcatatt ataaaaagta gtttttcctt ctttttcatt tgaaactgat cttgctataa
    21601 gagtagcttg agttgagtgg aatccagaaa caataccaca agcaactgta acaaagaatg
    21661 taggaataag aggaatacca tcaggatgaa tacctttcca atttgcaaga cttaaattag
    21721 ctaaatcata accttgtgta aagattccaa ttccaacacc agcagcagat aaaagtagaa
    21781 tagctccaaa tattggatat acctttccta tgattttatc aattggaaat agtgttgcac
    21841 acaagtaata tagaaagata agtccataaa ctatccagac aactggatta tttatagtcg
    21901 atttcatatt taaaatttga gtaacaacta aatccccagg tgtataaata aaaactgcac
    21961 caactaaaag cattaaaaga caaaggaata tattatagac ttgaaatact ttattaccta
    22021 aaaatctact gataagagaa ggcatttgtg ctcctttttc tcttatacta atcattccac
    22081 tcatatagtc atgcaaagct cctccaaata cacagccaat aggtattagt atgaatgcta
    22141 ttggaccaaa caatatacct tggataggtc ctagaattgg tcctgtacca gctatattaa
    22201 gcaactcaat tagactattc ttccattttt tcattgggac atagtctact ccatcacttt
    22261 gagcaagtgc aggtgttttt ctgtcatctg ggccaaatac tttttcacaa taagcaccgt
    22321 ataaagcacc tcctattagt aatatagcca agccgattaa gaatgttgtc ataatatcac
    22381 cccatttata aaattttttg gtctttgtat acatattatc taaatgtgta gatttatatt
    22441 gaatttaatt ctggacaacc tacaaattct ataaagtatt acaactaata taatttcaga
    22501 aataaataca tatacataac catgtactta gtatacaggt atttggtaat attttcaatg
    22561 ttatagcaat aaagaatata aacaaaattc tgataatatt ttacaaaaaa attaaaaaat
    22621 ataaaaatta gtttaaatag atggagaaaa aggaaaatca attgagaata taaatgtcta
    22681 tatcttaaca atattaagag gtttaaaatt cttgaagaaa gaatagtcaa gtgatataat
    22741 gggtttacag gtgtatatac actagtacat atagaaagcg gtgcaattat ggataaagat
    22801 ttaacatatc aaatcaaaga gctaaagaaa gaaaaaaatg caataatact tgcccatttt
    22861 tatcaaccac cagaaataca agaattagca gatgcagttg gtgactcata ttatttaagt
    22921 gagattgcta gagattgtaa agaagaagtg gttgtatttt gtggagtaag gtttatggga
    22981 gaaagtgcta agatattatc acctgaaaaa actgtattga tgccagtaag taacgctggg
    23041 tgtgctatgg cagatatggt agatgaagaa ggcgtgataa aattaaaaca acagtatcca
    23101 aatgctttgg ttgtatgtta tataaactct acagctaaag ttaaggcaca ttgtgatgtt
    23161 tctgttacat cttctagtgc aattaaaata ctagaaaaca tagataataa agaaattata
    23221 ttccttccag ataaaaattt aggaggatat attgcagaac aatttccaga taaaaacttt
    23281 atattttggg acggatattg taaataccat aacaatataa gagctgaaga aataattgag
    23341 ttaaaagata agtataaaaa tgcagaagta ttagtacatc cagaatgtaa aaaagaaata
    23401 agagatttag gagattatgt aggtagtaca tcaggaataa taaaatatgc tactaatagt
    23461 aaaaacaaag actttataat agcaactgaa gaaggtatat tacatgaatt aaagaaaaat
    23521 aatcctaata aaaactttta tattcctggt ggaaaaatac tatgtacaga tatgaaaaag
    23581 acaacattag aaaatttata tagtacactt aaaaatatgg aaaatgaagt tattgtagaa
    23641 gatgaaatca tggaaaaggc tttaaattca ctgcttaaca tgcacaaatt agcagaagga
    23701 tagagggata gacttatgaa tttagaacaa gatgttttaa tagttggaag tggagtttca
    23761 ggattatact gtgcattaaa tttagataaa agtttaaatg tgctagtagt atcgaaatca
    23821 acaattgaaa acaacaatac atatcttgca caaggtggaa tttcaactgc tagaaatatt
    23881 gatgatatag aatcctttgt agaagataca atgaaggctg ggcagtataa aaatagagtt
    23941 gaagcagttc aagtacttgc agatgaatct atagaaaatg taggtcagat agtagaatat
    24001 ggtatgcctt tagacaaaga aaatggagag atagattaca caagagaagg agcacatagt
    24061 gtaaatcgaa tagttcatag taaggataat actggagaag ttgtttttaa aactttatta
    24121 aaagaagcta aaactagaga aaatataact cttatagaag atgcatatct tttagatata
    24181 ttaaaagatg gcaataaatg tataggagct agaattttta aatctaaaaa agagattcat
    24241 gttttttcaa aaatagtagt tttagctaca ggtggaattg gtgggctttt taaaaattct
    24301 actaatcaaa gacatcttac aggtgatgga attgcaatag ctttaagaaa taatataaaa
    24361 atagaaaatt tagaatatat tcaaatacat cctacagctt tttatgaaga aaataatgaa
    24421 ggtaggagaa tgcttatatc tgaatcatta agaggagagg gagctaaact tttaaataaa
    24481 aataaagaaa gatttgtgga tgaacttctt cctagagatg tagtttcaaa agctattttt
    24541 gagcaaatgg aaaaagataa attaccttat gtgtatttag atgcaacaca tttagatagt
    24601 gaatatttaa ttaatagatt ttcttttatt tataatgaat gtttagctag aggaacagac
    24661 ataacgaaag aatgtataaa ggtatctcct gcacaacatt attttatggg cgggatacat
    24721 gtagatttgg attcaaaaac atctatggaa aatttatatg cagttggtga aattagttgt
    24781 acaggtgttc atggagccaa tagattggca agtaattcat tacttgaagg gcttgtattt
    24841 tcaaaaagag cagctaaaaa tataaactca gttatagata atgtaaaagt taagtttata
    24901 gatgtaccag atatggatat agatatagaa caggtaaaaa aagaaaataa aatattagtc
    24961 ataaaagaaa ttgaaagaac aagtgaggat tttggcgatg aattatttga ttattgatag
    25021 aatgatacag gatgctttga tagaagatgt tccaagtgaa gatataacaa ctaattctat
    25081 tgtggatgaa aattctaaat ctactgtaga tttaatttgc aaacaagatg gtataatagc
    25141 tggcttaggt gtatttaaga gagtttttga gatacttggt gatgtagatg taaaattata
    25201 taaaaatgat ggtgatagag taaagaatcg tgaaaagata gctgtattaa caggtagtac
    25261 tagaaactta cttgtaggag agcgtgtagc attgaattat ttacagagaa tgagtggtat
    25321 agctactatt acaagtaggt atgttgaaaa gcttgaaggt acaaatacaa aattattaga
    25381 tagtagaaaa acgataccta atcttagaat attagataag tattctgtta aagtaggtgg
    25441 aggatgcaat catagattta atttatctga tggaatattg cttaaagaca accatattgg
    25501 ggctgctggt agtgttaaga aggcagttga attagctaga aaaaatactt cttttgttag
    25561 aaagatagaa gtggaagtgg aaactttaga catggtaaaa gaagcaatag aagctaatgc
    25621 agatataata atgttggata atatgagttt ggaaatggca aaacaagcag tagatattat
    25681 aaatggaaga gccattgtag aattttccgg aaatgtaaat ttagatacta tagaagatat
    25741 aggaaaaatt ggtgtggatg ttgtttctgt aggagcgctt acacattctg ttaaagctct
    25801 cgatataagt atgaagaatt tgagaaatat ataaatttta tattgaaata aacttaatta
    25861 aaatagactc cttaaatttg ttttataaat ttaaggagtc tattttttat tcattaaaat
    25921 taataaaaat agaaaaaaat caatcaataa tttaataaaa atagataaat actatataca
    25981 cttttaataa tatagttaat atctatttta cagataaata agaataaact tataatataa
    26041 ttgtatctgt aaaattttgt aaaatataaa aagtataggg ggaataaaaa tgaaagtagc
    26101 ttatattttt agtagtacta atacacataa gattctagac aaaatgataa taccacaact
    26161 tgaacaagga actcatggag tagacgttct agggatgttt ttctttatgg ataatacctt
    26221 atttttatct aaaggtaatc ctgtaggcga gagactaagt aaattacatg aaaaaactga
    26281 tatgataata atggcttgtg accaatgtgc tatagaaaga ggtatagaga ataatttggt
    26341 tgatggagct acaattggat gtttcccaaa cctatatgct tgtttaggca gtgcaggtgt
    26401 ggaccaagtt ataacgttat aaattagtat aggatacaat ccatagaaaa atttattttc
    26461 tgtggattat tttaagtaaa agataaattg tttaaagtag gggattaaca aatgtctaaa
    26521 attttagttg aatttataaa cacttgacca tcttgtgatg aacatggagt gtttataaaa
    26581 aaaatgtcag aaaagtatgc tgataaattg gaaataaaat tatatcaagc agggaaagat
    26641 ttttcgtata taaaaaaata tgggattata acaaaaggaa ctttaataat aaatcagaaa
    26701 aagaaatatg atagattaaa taaagataca attgaaaggg caatagtaga agctataaac
    26761 aataattaaa agtttaaaaa ttaggagtat caaaaatgaa tatattagaa attgttttaa
    26821 atggagttaa agatactata gtagcttcta tagttacact gaatggagca tcaggatggc
    26881 ttatgataag ttttatattg gcaggtgtac ttcacaatgt tttaactcca agaaggtttc
    26941 aaaaacaact tggaaataag aaattttctt ctttaattaa aagtactatc tcaggaatgt
    27001 ttttaccaat atgtagttgt ggagtaatac cacttggaat aagtatgtat tattcaggag
    27061 catatttagg accagtactt gcttttatga catcaactcc aataataaat ccaatagctg
    27121 tagttctttg tctaggtctt ttaggtccta aagttacaat catttatcta atagctggtt
    27181 ttttagtacc atttttagta ggatttttag gaaattgttt tggaaaagaa gaattatcac
    27241 ttcataatga ttcagaggaa gaagtaatag aattagaaga agaaaaaaga agttttatgg
    27301 gaaatatgat tgatggtctt aaatggtctt ttggtgaatt agcccttact attagtaaat
    27361 atgtagtaat gggtatggtt tttgcaggat ttataacaac tatttttcca aatagtataa
    27421 ttcaaagata tttaggaaat ccaggtttgc tatctcttgg aagtatagca atacttgcat
    27481 gtgttatgta tgtatgtgct gtaggacata taccgtttat agcagccctt gttgcaagtg
    27541 gagcatctcc tggtatagcg ataacatttc taatagctgg agcagctaca aatctaccag
    27601 aacttataag tatgtttaaa cttattggaa aaagaactgt tattatatat tctgtaactt
    27661 tgacactgtc ttcaataata attggatata taacaaattt acttttagtg aataataact
    27721 cagtaaattt agatgcaaca aattcttcaa tacagttggc aaataaacta atgttaaaca
    27781 tacctgatag ttttgagtat gtatgtacta ttttaattat tttatttgca tttaaatcaa
    27841 taataccaaa gatgaaagag gtcataggaa atcatgctta aaaagatagt tataatatcc
    27901 tgtatagtag ttgtgttaat cattttatca aaaatagttg atgataacat caaagaagat
    27961 gcttctattc caaatgtgaa taaagaaaca ttagaatatt ttagaaaaaa ttataaagaa
    28021 gacataataa cttgtgctga agaagactta aataatgatg ggaaaaaaga tttagtagtt
    28081 atatataaaa aaagcaataa ttcaaatgaa atggtagtcg tagtttctga taaaaatagc
    28141 cactatataa caaagccaat accagcacct atagagaatc aaactataac ctttaaaaat
    28201 attgatgata aagcaccaat agaagtaatt gtatcagggt ctaaaaatgg taatgtagga
    28261 tatgctattt acagagtaga gggtaaaaaa tttgttgatt tatttggcga agatatggac
    28321 aaatgttgct aaaaataaaa ataatacata aaggtgggaa atttatatga aattatctaa
    28381 gaagattcaa gcattaatct tatttggtgt aatgggtaca tctatattaa caggatgtag
    28441 tagtaaacca aaagaaaaag aagaagcaaa aactggaaaa gatgattata caataacagt
    28501 aggttattat aattgtgacc atatgactgc gggaccagta gcagaatcgg caggaatata
    28561 taaagattta ggtcttaatg taaaaactgt tggaaatggt aaagttcctg aagctatggc
    28621 agcaggaaaa atggatgcag gttacatagg aactaagggg ttagttggag caatacctaa
    28681 gggttcgcca ataacaatag cagctaacaa ccatactggt ggttctgaat atttaatagt
    28741 ttcaaaagat ataaaagaac ctaaagattt aataggtaaa aaaattgcaa cagatatgag
    28801 tgatttctta tggacaagtg attatggtcc agaaacaggt ctaccaactg acccatcaaa
    28861 atatgaagtt gtaaatatgg attcagacaa agataaatat ttagctctta aaactgggaa
    28921 aattcaagct tttacatctt gtgacccttg gggttctgta gctgaaaatg acggtgcagg
    28981 aaaaataata gcatctactc aatataaaga aaaggctaat ggcaaagaat ataactgttg
    29041 ttcattctca ttaaacaaaa actttataaa agaacatcca gatttagcaa aaaaattggt
    29101 tctagcacat acaaaagcaa tagaatatat atatacaaat ccagctgaag cagctaaaat
    29161 atttgcaaaa tattataatg tagaagaaga agttgcactt agaactatct ataagaagac
    29221 tgttggagaa ggtagaactc ttacttggaa agttacaggt gaagaatata aaaataattt
    29281 acaaatgtat aaagatttaa aggctttaga tgatatacct aaatatgaag atacgattga
    29341 tacaagctta ttggactctt gtggagctga tgattttgac aaatttataa aagaaaaagt
    29401 taacccagag tttcctgaag gtatgagtta tgaagactgg aaagcaaagg tattataaat
    29461 aaatggagaa tatgaaaaat ataataatac tttttatcat tattttggga agtatattta
    29521 taatattttt taattttgaa gataaagaag aaataaaagg aaaattaaga ataggggtat
    29581 ctgatgatac ctctggtttt gtaataaatt atatggtaaa tagagactac tttgaaaata
    29641 taaaagtaag tgatataatg gatattttta caattaatga ttgctgagca agtacaatgc
    29701 agtgggctct gagttcagag gaaatagata tagcaatcat atgtaaagat gctgcaaaac
    29761 aatatgtaaa tgtaaatagt ggatttgaaa ttgttggtac tgtagttcaa aattctgata
    29821 tatttctatt aagtgataat aatcctgaaa agataggagt cattcaaaat agagattacc
    29881 agtcagaatt ggttaaaaaa tactacaaga atgtggaagt agctccctta ttagggactg
    29941 cactagcata tggattagaa agtaatttag tagatggtgt tgtaatagat tgcataaagt
    30001 caattgggtt agagggtaaa agaaaatcta caactaaatt aggagattat gatacctatg
    30061 tattagttgt aaataaagag tttaaaaaaa gtaaagcata tagtgatttt ataacacttt
    30121 acaataaaag tgttgatgct ttaaagaata aagctacatt taagaaagta ttaaaagagt
    30181 acaggaatat aagaaatata gatatggagg aaataagcaa ttggaaatta aaatttctaa
    30241 agataaaagc gatagattaa ctaagaaaaa aactgatggt agtatggata ttttatataa
    30301 aataatacta ttagttatta tatttttagt ctggcatttt gcagcaaaag atatagggag
    30361 ttctttgtta cttccaatgc cagtagatgt aataaaaggt ttctttttct gtgtgacaga
    30421 tgcagagaca gtcactaatc tttttataac attgcagaga gtacttaaag gttttatgta
    30481 tgcattattg tttgggcttc caataggatt tattatggga ttttctaaaa cttttgaaag
    30541 ggttttatct ccagtagtag attctgtaag gcaagttcca ataatggcat gggttcctct
    30601 tactatagta tggtttggta taggagatgg tccaacaata tttttaatag ctttttcagg
    30661 ggttttccct ataattttaa atactattca aggagtaaga gcaatatcaa aggattatta
    30721 taatgcagct agaagtatgg gagcatcgcc aattgtgata tttacaaatg taattgttcc
    30781 cgcatctctt ccagatatac taactggttc aaggattgct ataagtacag gatggatgtc
    30841 tgttatctga gccgagttta tagctacgag tgctggtctc ggttactcaa tggtacaagc
    30901 tcaaacatta atgcatacag atgtattaat tgggcttatg atatttgcag ctttaatagg
    30961 atttataata gatagggtat taaagtttat aaataaaatc ctttgtagat ggaggtttgc
    31021 agattaatga agttaagtgt aagagatata aataagacat ttgtaaataa tagagtacat
    31081 acaaaggttt tagaagatat aagtatggat atagatgatg gacaatttgt ctgtctttta
    31141 ggaccatcag gttgtggaaa aacaacttta cttactataa taggtgggtt tcaaaaatca
    31201 gaacgtggag atgtttttat aaatgataaa agagttaaaa aaccgggcat agatagagct
    31261 tttatatttc aaaattatgc tttatttcct tggaaaacta taagaggtaa tgttctattt
    31321 cctatgaaac agcagaaaat accaaaagaa aaaagagaag aaatgctaga agaacttttg
    31381 gttatgtcag acttgaaagg aaaagaaaaa ctgtttccac atcaattatc tggaggaatg
    31441 aaacaaagag tggccatgat aagagcttta gcatgtaatc cagaggtatt actaatggat
    31501 gaaccattag gagctgtaga ttttcaaatg agacaaaatt tacaggaaga gctagaacga
    31561 atatggataa agaagaaaat aactgcatta atggtaactc atgatgttga tgaagctgta
    31621 tatatgagtg atagagttat agtaatgtcg agagataaag gaagaattat tgatgatata
    31681 aatatagata ttccaagacc tagaattaga ggaagccaaa aatatgaaga gtacaaaaac
    31741 aaattaactg atactttatc aaagtgttat gaagtttaaa gaggagtaga catgagtcaa
    31801 atagaaaaat acaatagttt ggataataca caacagaaaa taagaatctc tattatggat
    31861 atgataatag ataaaggttc tagtgttaca cttcaagaag tgactgaata tataagtaaa
    31921 aagttgaata tagaaaaaga atatatagaa aggacactac aatattttat ttataaaaat
    31981 ataatggttg ttgatggaaa tagtataaat tttatatatc ctgtatcagc attgccaaca
    32041 aatcataaag taactttgag agataataga agtttcagtg caatgtgtgc tatagatgct
    32101 ataggaacct cttgcacatt caaccaagat ataaaaataa actctatttg tagtgctaca
    32161 ggtaaggaaa tagaagtaat tataaaaaat gaaaagattg agtatgtgaa taatccaaac
    32221 ttaagagtgc ttcatataaa tcttgataag catctaaatt gggctgctag ttgctgaaat
    32281 ataatgaatt tcttctggac gaagagtgat tttgatgctt ggacaaacga agcgggatta
    32341 tctgatgatg aagatatata ttgtttagat attaatgagg caatagtaga atcatataaa
    32401 atatttaaac tcaaacaaaa ggtactatca tagatataca tatatgaaaa actaaaaatg
    32461 acctttagat aaaaatgata ggtaatatcc atactatata aattagtata gagtagggat
    32521 gtgaaaagta tttaaatcta aaggtatatt tttagttttt ttagatgatt agatagcctt
    32581 attaagtata tatttattta taacctttgc aactccattt tcattattag tatcagtaac
    32641 ataatctgca atttccttta tactattacg tgcattcccc atagccacac caagacctgc
    32701 atactttatc atatgttggt cattttcttc atcgccaaca gcaatagcat tttctatagg
    32761 tatatttaga ttcgtacata atgttttaag agcagttcct ttattagtag ttttacctaa
    32821 aaattctaga taaaaaggag cagaccttac tatagtatat ttattgaata aatttttagg
    32881 tagttgctgt atgatttctt ccaatctttc tggttcatca actatcataa ttttacagat
    32941 tttaatagat tcatcaattt tatctttttg catatatttg acttctatat tatttaattt
    33001 cgcttcatga agtgtatatt tgccaggcgt ttcatttggt gtaattaaat ttgtcattgt
    33061 atttatgtgt attttagtct tatatttttt acaaacgtta ttatatataa aatcaaaatc
    33121 atctaaactc atttctacat catgtatgat tttctcactt ttaacttctt gaataattgc
    33181 tccattaaag catataacat aatcaccttc atctgttaaa tttagttgat ttagaagttc
    33241 ttttacacca ggaagaggtc tgcctgttgt tataactact tttgccccag ccctttttgc
    33301 ctcctgaatg gactcaaaga cttcaggagt aatccttttt tgtgtattta atattgttcc
    33361 atcaatgtct agtgcgatta atttgtacat atcaacctcc aataaattat atactcaaat
    33421 ttcctgtgaa aacgttgaca cagctagtgt tgaacaaagt ttcagtggga aatatggttt
    33481 gttttgtata ctattatatc aagagataag taatttagaa agtttaataa tattaatttt
    33541 ttgataaaaa tattacttat gtgagatgag aatgtaaaaa ctttgtaatc aataattcca
    33601 aaattcttaa ttacaaataa tggtatcgtt tgcattgtta atataaataa atgtatatta
    33661 tatagttaga agtttgaata aatagactga tggtaatttg ttatattagt tagttttgtg
    33721 ttataaaata ttgtaattcc attaatcata tgttattatt caacatgatt ataatataaa
    33781 gaatatttga tatatataat aatttataaa agagtattac atagtacatg taaatctata
    33841 tttataaata caataaagta aagttgtaaa ggagtgcttt aaatgaagtt ttcagagttt
    33901 aagtatgaaa gacccaatta taatagtatg aaaaaagagt ttttgagttg tgtagaagat
    33961 ataaataatt ctcgtaatta taaagagcaa caaaaaaata tacataagat taatttatta
    34021 agaaataaaa tagaaacgtt gtcaaatata gcgtcgataa gatatagtac agacacattt
    34081 aataaattct acaaagaaga aaaaaattat tgggatgaat atatgccttt gtatgaagaa
    34141 ttaaattcac atttttataa tgccattgta aattcaaaat ttaaatatga tttgataaaa
    34201 gagtttggag agcaattttt cacaatagta gagtattctt taaaaagttt ttccaaagaa
    34261 ataataagtg aattgcagga agaaaataaa ttgtgttcag agtatactag acttttagct
    34321 tcagctgaga taatgtttga tgggaaaata agaaatttat ctggtatggg gaaattcatg
    34381 tattctaaga atagaaaaac aagagaatta gcaaacaaag catattataa tttctttgaa
    34441 gaaaatgaaa ctaaatttga tgacatattt gataaattgg ttaaacttag agataagatg
    34501 tctaaaaaat taggttttga agattttgta gagcttgggt acgttagaat gatgagaagt
    34561 gactacagag aatatatgat aaaaaatgtt agaaaacaag tactaaaata tgttgttcca
    34621 atggcaaatg aactttatga aaaacaagct aaaagaattg gtctagaata tttaagttac
    34681 atagatgaag gcgtggaatt tttaacagga aatgcaagtt taaagggaga ttcaagatac
    34741 ataattcaaa atggtaaaag gatgtattct gaactgtcaa aagaaacaaa tgaatttttt
    34801 gattttatgt tagaaaatga acttatggat ttagaaacga agaaaggaaa aggagctgga
    34861 gggtattgta cttatatacc agattataaa tcaccattca ttttttctaa ttttaatcaa
    34921 actgctgatg atatagatgt gttaacacat gaggcaggac atgcatttca gttgtatatg
    34981 tctagatgga ttgatatgcc agaaattaat tttccaacgc ttgatagttg tgaaatccat
    35041 tcaatgagta tggaatttat aacatggccg tggatggatt tattttttaa agaagataca
    35101 gataagtaca aattcactca cttatcatct tcaattaaat ttatacctta tggagtaatt
    35161 gttgatgaat ttcaacacta tatttacaaa aatcctaatg tagataagtc aaaacgaaaa
    35221 gaaatctgga gatttttaga gaaaaaatac ttacctcata gaaaatatgg tgataattct
    35281 tttttagaga gaggttgttg gtggtttaaa caaggacata tatttaaaaa tccgttttat
    35341 tatatagatt atgtattagc tcaaatttgt gcgttacaat tttggaaaaa aatgattcaa
    35401 gatagagatg caggatggaa agattatata aatatctgta aagttggagg aacaaaatca
    35461 tttttagata ttgtgagtat gggaaatttg tattctccat ttgatgatgg ctgtattgag
    35521 tctattatag gtgatgttaa atcttggttt gatgaaataa atgacagcaa attataaaaa
    35581 attatgagaa ttttattgta taaaagtgaa aaattaaaaa atatataaaa agtgattatt
    35641 tgttgtgaaa atagtcttgt ttttaactaa attgataaaa taaactaaaa aagatttgta
    35701 cttaaaaaaa aaagaaaata caagaaatat gcaatatcaa catttggaaa aaaaagtaaa
    35761 aatgtataca aaatacatat aaaaaaacta ttgcaaattt tatagaatgt ttatatactt
    35821 aagtcaacag agatagaaaa aagctattta tagaaaacaa aggataagtt gcttaatttt
    35881 aaattttctg aaaaaaataa taatccggat gaagagtatg ggagactgct ttataaaaag
    35941 cgaccgaagg gacaagttta atcttactta cccaaaagat tagatgaaat tctcaggtaa
    36001 aggtgactgt acttggacgg ggctctgaaa atgattttaa cagggaagac aaattgggag
    36061 atttgttttt cctgtttttt atttatcaag taataaataa aagtttgaaa gagagtaaaa
    36121 aaatgataat aattacgaat aatccaaaag taaaagaaga agttcaaggc agagaagttt
    36181 tgtttaaaga cacaacttat attggaatct tagaagcaag tagagatttg atacatgaag
    36241 gttatgaact tttatctcat ccactctatg ggagtgtaaa gccaaatgaa acaccttaca
    36301 ggactgttat actaaagaaa ggaaatcgtt tggatataaa ctctttaacc ttaattgaag
    36361 aagcaatcat cactgcaagt aaatttcaga ataataaaaa gactccaaaa tggacagaaa
    36421 gtgttcaaga tgattttaga gtaattgatt atgatatatt ttataataca atacaaagaa
    36481 tgcagtatga ataaaaaaaa ttgttaaaaa gttattaaaa cagcctaaat attaaatcta
    36541 atttaatatt aaataaaatg agaaatttaa ttggggagga taagtcaaaa tggaaaatgt
    36601 gtatgactta gtaataattg gttcaggacc agcagggctt gcagcaggtc tttatggagc
    36661 aagagcaaag ttaaaaactc taatcctaga aaaagacaag actggtggac aaattgtaat
    36721 aacacatgaa attgcaaatt atccagggtc agttccaaat gcaacaggac caagtttaat
    36781 agctagaatg gttgaacagt gtaaagagtt tggagcagaa atgctaagag acaacattgt
    36841 tgatactgag ctagatggag atataaaagt tttaaaaggg gaaaaggcag aatatagagc
    36901 aaaagcagta ataataggaa caggagcaac accaagaaag attggttgtc ctggagaaaa
    36961 agaacttact ggtaaagggg tttcatattg tgcaacttgt gatgcagatt tctttgaaga
    37021 ttttgaagta tttgttgtag gtggaggaga cagtgcatta gaagaagcaa tgtatttaac
    37081 taaatttgct agaaaagtaa caattgtaca tagaagacaa ggatttagat gtgctaagag
    37141 tgttgaagaa aaagcaaaag ctaatccaaa gatagagttt ttattagata cagtaattga
    37201 agaaatcaaa ggtgatggaa tattagaatc agttgtattt aaaaataaag ttactggaga
    37261 aactcatgaa tactttgcag atgaagaaga tggaacaatg ggagtatttg tgttcgttgg
    37321 attagatgct caaactgatt tattcaaagg taaagttgat atggatgaaa aaggttacat
    37381 aataactgat gaagatatga gaactaacat accaggcgta tttgcagcag gagattgtag
    37441 atctaagact ctaagacagg ttgtaactgc aactaatgat ggagcaattg catctatagt
    37501 agctgagaaa tacattgatg aaaaatttgg gaactagaat ctgtaaatta agaaatagtg
    37561 tctgtaaatt taatgaataa tatttaaaat taaaattaat atttaaaatt aaaataaaaa
    37621 tttcaagggg gaataagaaa atgttagatt tagataaggc tacatttgaa gaagaagttt
    37681 taaatgcaga aggttttgta tttgttgatt tttggagtga aggttgtgag ccttgtaaag
    37741 ctttaatgcc agatgttcat aaattagctg agacttatgg ggataaaata aaattctgta
    37801 agatggatac aacaaaagct agaagattag ctataaaaca aaaagtatta ggtcttccaa
    37861 ctatggctat atataaagat ggagaaaaag tagacgaagt tactaaggat gatgctacag
    37921 ttcctaatat agaaaacatg attaaaaaat atctttaata tgttgataaa ttattaacat
    37981 taaaagaaaa aatctatttt tatctgaaga taagaagaaa aaggttttta tgtccaactt
    38041 tgttaaaaaa ataattttaa ataaaataat ttttttacaa taaatatttt ttagtaataa
    38101 aataaagatt taaggcataa aaacacaata atatatcttt aaaaaaataa gataaattgt
    38161 aaaatgatat aattttgaga aaaactcaaa tgcatataca caaattaaat aacaggaggt
    38221 gcaagtatgc gtcttgaatt agggaaaatc tttataaaag atattcaatt tggtgatgta
    38281 acagaagtaa aagatggagt attattcatc aataagcaag agatgctaca agaaattggt
    38341 ggagatgagc atattaaatc tatagatata gaattagctc gtccaggtga aagcgtaaga
    38401 ataacacctg taaaagatgt tattgagcca agagtaaaag ttgaaggtaa tgggggaatc
    38461 ttccctggta tcatgtctaa agttgacact gtaggagaag gaaaaactca tgctttaaaa
    38521 ggtgtagctg ttgtaactac tggaaaaata gtaggtttcc aagaaggtat aatagacatg
    38581 actggagaag gagctaagta tactccattt tctaaattaa ataatgtagt agtaatagct
    38641 gaacctattg atggtttaaa acaatacgct catgaaaaag ctgttagaat gataggattc
    38701 aaagcagcaa tgtacttagg agaagtagca agaaatctta ctccagatga agtttcagta
    38761 tatgaaacta aacctcttct tgaatcaata aaagaatatc cagagcttcc aaaagtagga
    38821 tatgtttaca tgcttcagac tcaaggttta ttacatgata cttatgtata tggagtagat
    38881 gcaaaacaaa tagtaccaac tttattatat ccaacagaat taatggacgg agcaattgta
    38941 agtggtaact gtgtttctgc atgtgacaaa aacccaagtt atgttcatat aaacaatggg
    39001 gttgttgaag acttatatgc aagacatggt aaagatataa actttgtagg tgtaattata
    39061 actaatgaaa atgtttacct agcagataaa gaaagatctt caaactggac ttctaaatta
    39121 tgtaaatatt taggattaga tgcagttata gtttcacaag aaggttttgg aaatcctgat
    39181 actgacctta taatgaactg taagaagata gaaatgcaag gtgttaaaac agttatagtt
    39241 acagatgaat atgctggacg tgatggaggt tctcaatcac ttgcagatgc agatgtaaga
    39301 gctgatgctg ttgtaacagg tggaaatgcc aaccaagttg tagttcttcc taagttagat
    39361 aaagttattg gacatttaga agtagtagat gtaatagctg gtggttctga tggaagttta
    39421 agagctgatg gaactattga agttgaaatc caagctataa caggtgcaac taatgaaaca
    39481 ggatttggtc atttaactgc taaaggatat taagaagtat tttattaaac gattgagtaa
    39541 tgttaataaa acatattata gataattata ataagtgtta tagatatata aaaatatatt
    39601 agaaatataa taaggaggat tattatgagt ttacttagta ataaaaaggt tcttataata
    39661 ggtgaccgtg atggtatacc aggacctgcg atagaagaat gtgtaaaaac agtagaagga
    39721 gcagaggttg ttttctcatc tacagaatgc tttgtctgaa cagctgctgg ggctatggac
    39781 ttagaaaatc aaaacagagt taaagatgct gctgataaat tcggagctga aaatgttgtg
    39841 attttactag gtgctgctga agccgaagct gcaggtcttg cagccgaaac agtaactgct
    39901 ggagatccaa ctttcgctgg accacttgct ggagttgcct taggattaag tgtttaccac
    39961 gttgttgagg aaccaataaa atcattattt gatgaaagtg tatatgaaga ccaaataagt
    40021 atgatggaaa tggttttaga agttgaagaa atagaagaag aaatgtctgg tataagagaa
    40081 gaattttgta aattttaatg cctaaaagca aaaggggtga aactaagaga tgggaaaact
    40141 aaaagcagta cattatatta atcagttctt tgctggtata ggtggagaag aaaaagctga
    40201 tactaagcca catgtggcag aaactttacc tccaataagt ttacaacttg ataaattatt
    40261 aggtgaagat attgaaatag ttggaacagt agtttgtgga gatagctatt tcaatgagaa
    40321 tatagatagt gcaagtgaag aagttttatc aatggtaaaa ggttttgaac cacaactttt
    40381 catagctggt cctgcattta atgctggtag atatggggtt gctgctggaa caataactaa
    40441 agttgtaaaa gatgctttga atataccagc tttaacaggt atgtatgttg aaaatccagg
    40501 agcagatatg ttcaaaaagg atgtttatgt agtagaaact tctgattctg cagcaggtat
    40561 gagaaaggca cttcctaaga tagctaaact tgcagtaaaa cttgctaatg gagaagaaat
    40621 tggaactcct aaagatgaag gatacatagc tagaggaata agagttaact acttccatga
    40681 agacagaggt tctaagagag ctgttgacat gttagttaag aaaataaaag gtgaaccatt
    40741 tgaaactgaa tacccaatgc caaactttga tagagttgac ccaagtaaag ctgtaaaaga
    40801 tttatcaaaa tgtaaaatag ctttagttac ttctggtggt atagttccaa aaggaaatcc
    40861 agatagaata gagtcttctt cagcttctaa atatggaact tattctatag caggagttat
    40921 ggatttaaca gaagagactt atgaaactgc acatggtgga tatgacccag tgtatgcaaa
    40981 tttagatgca gatagagtat tacctgttga tgttcttaga gatttagaaa aagaaggtgt
    41041 aattggaaaa ttacatgaga ctttctatac aactgtaggt aatggtacat ctgttgcaaa
    41101 ctctaaaaaa tatgcttctg aaattggagc agcattagtg gctgatggtg tagatgcagt
    41161 aatattaact tctacatgag gtacttgtac tcgttgcggt gcaacgatgg taaaagaaat
    41221 agaaaaaact ggacttccag tagttcatat gtgtacagta gtacctattt cattaacagt
    41281 aggggctaat agaatagttc caactatagc aatacctcat ccacttggaa atccagcgct
    41341 tgaccctaca gaagaaaaag ccctaagaag aggccttgta gaaaaagcat taaatgcttt
    41401 aactacagaa gtagatggtc aaacagtatt tgaaaaataa aaataacaca cacatatatt
    41461 atatagataa aaatataaac acacactaaa aataaaaaat aactatacac aaaaaaaata
    41521 atatagattt catgaatgga taaattggag ttattcattc atgaaatctt aaaaaatgcc
    41581 aaaatacata aacaataata gaatcaaata tacaataaaa tacaatgatt agagctttta
    41641 ataagtttaa taagattaat aagattaaaa aataagtgtt cataaaattt atggaggtgc
    41701 acaaaatgac ttatccagtt ttaaaagggg caggctatgt acttattcat actccagata
    41761 tgatagtgca aaatggaagt acttgtacag ttgaaagagc aacaaatcca gattcagaat
    41821 ttttaaaaga agtaagtaat catataagaa gttatgaaga tgtagttaac tacatgccaa
    41881 atcaagttta tataggaaat agaagaccag aagaattaag agatttacca atgccatggt
    41941 gtgaacaaaa gattgaggga actagaaatg gtaagtttgg agaaataatg cctcaagatg
    42001 agttcatagc tttaatgcaa ataagtgatg catttgattt agtaaaatta tcacaagaat
    42061 ttattgatga agtaaagcca aaaatcgaaa ataactaccc tgaaattgca ccatttgtag
    42121 gtaaattaaa gggtgatgat atagaagaag gtaaagaatt agtagcaaca cacatagctg
    42181 aaggattata ccatgatgga aaatttgttg gttatgtaaa aagagcacat gatgtagatg
    42241 ttaacttaaa tgcccataca atgtttgaaa atctagtagt aaaagcatct ggagttttat
    42301 ctgctattca aatgctaaga catagcaaaa tagaccctgc tgaaatagat tatgttattg
    42361 agtgttcaga agaagcttgt ggagatataa accaaagagg tggaggaaac tttgctaaat
    42421 caatagctga gatagcagga cttcaaaatg caactggttc tgacacaaga ggattttgtg
    42481 cagcaccaac tcatgcatta attcaagcag cagctttagt aaaagctggt atacataaaa
    42541 atgtaatggt agtagcaggt ggagcaagtg ctaaacttgg aatgaatgct aaagaccatg
    42601 ttaaaaaagg tcttcctgta cttgaagatg ttgttggagg atttgcagta cttgtatctg
    42661 aaaatgatgg tgtaaatcca gttataagaa ctgatttaac aggtaagcat actgttggaa
    42721 ctggttcttc accacaagca gttatgacag cacttataac ttcaggtctt gatagagcta
    42781 atttaaaaat aacagatgtt gatgtgtatt cagttgaaat gcaaaaccca gatataacta
    42841 agccagcagg agcaggagat gttccagaag caaactacaa aatgataggg gcacttgctg
    42901 ttaaacgtgg agacttagaa aagaaagaat tgaaagattt tgtttctaac aaaggattac
    42961 caggatgggc accaactcaa ggacatatac catcaggagc tccttatata ggatttttaa
    43021 tagatgattt aactacagga aatagaaata gagctatgat agttggaaaa ggtagtttat
    43081 tcttaggaag aatgactaac ttatttgatg gagtatcttt tatagctgaa agaaatacag
    43141 gtgttactga agagacttca ggaatatcta aggatgaaat taagaagata atagctgaat
    43201 ctatgaaaaa actagcatta gatatgttag aagaataggg gtgaggatat gtcaaaaaaa
    43261 gtaatcgcag atgtattctt agaagtagct aatgcaatag aaagcggcga atttggcaaa
    43321 aaagtaaaaa ttggtgttac tacacttgga agtgaacatg gtgtagaaaa catggttaat
    43381 ggtgcacaat tagcaaaatc taatttattt gatattctgc ttattggacc aaaagtggaa
    43441 actgaccttg aagtagttga agttaatgat gaaaaagaaa tgcatgcaaa aatggaagaa
    43501 ttattagatt caggatatat tgatgcttgt gttactatgc attataattt tccaataggt
    43561 gtatctacag taggtagagt aataactcca gcaaaaggaa aagaaatgat acttgcaaca
    43621 acaacaggaa caagtgctac taatagaata gaggcaatgg taagaaatgc catttatggt
    43681 attgctacag ctaaatcaat gggtaacaaa tgcccaaaag ttggaatact taatgttgat
    43741 ggagcaagac aagttgagaa gtgcttaaaa gaattaaaag ataatggata tgacatggag
    43801 tttgcggatt caattagagc agatggtgga tgtgtaatga gaggtaatga tttacttgta
    43861 ggtgcacctg atgttatggt tactgataca ttatctggaa atatatttat gaaagtattt
    43921 tcttcatata ctacaggtgg agattatgaa gctcaaggct ttggatacgg acctggagtt
    43981 ggagaagact atgatagaaa ggtacttata gtatcaagag catcaggttc tccggtagta
    44041 gcaaatgctt taaaatacgc ttatgatgtt gtaaagggag atataagtaa tgttgcaaga
    44101 aatgaatttg caaaggttaa aaaagcaaaa tttgatgaca ttatatcctc tcttactaaa
    44161 aaagaagtta aagctgaaaa agtagaagtt aaaatgccag ataaggaaat tgttactcgt
    44221 caaatagctg gtgttgatat aatggattta gaagatgcag tatctgagtt atggaaaaac
    44281 ggaatctatg ctgaaagtgg tatgggttgt acaggtccaa tagttttagt aaatgaagct
    44341 aaaggagact tagcagttga aactttagtt aaagctggat atactgcaaa ataagtacat
    44401 tttttatatg taaataagaa catatagtta taattttatt gattataatt atatgttctt
    44461 tttattttat atcttgtcaa tatttatata ttaacacaat ccatttattt gattctctag
    44521 ctaatttatt atatttattc aattaaaatt aagctttcta actaaataaa aactaatgaa
    44581 gtattaaaat gaaattgata aaatatgaaa aaattttagg aataaagtat ctgttagata
    44641 aaaataagat taattagtga tataatataa ttaagataaa atttattatt tggaggtaaa
    44701 atcatgagca gagtaaaaaa tgtagtagaa ttgttggaaa ctaaaggtgt tgatgcacta
    44761 tatctaacaa agaaaactaa tgtaaactat ataagtggtt ttcctgatga agaagcatat
    44821 gcagtgattt gcaaagatgg aaacttttta gttactgata gtagatatat ggagcttgca
    44881 gaaaaggttt gcaaagattt tgaaattata aattggcata attttgatag aagtgtggca
    44941 aaggcagtta aaagtgtatg tgataaagtg ggtataaaga aacttgggtt tgaaagaact
    45001 aatatagtgt ttgataaata tgaagaactt aaaaatctga ttgagaagga taatggagaa
    45061 ttaataccta cagaaaatat tgtagagact ttgagatatg caaaagacaa agatgaaatc
    45121 aaaaatacaa gaaaagcttg tgaaatagcg gataaggcat tagaagaatt gattccacat
    45181 ataaaagctg gagttagcga aattgaactg gctacaaagt tagagtactt tatgaaaatg
    45241 aacggagcac aaaatatagg gtttgaaact atactgatat caggagcaaa gacttcattg
    45301 cttcatggta agcctagtga taagattata gaaaaaggtg attttgtact tatagactat
    45361 ggtgctatgt ataatggcta tatatctgat acaactagaa cttttatagt tggaggagct
    45421 tctgaaaagc aattagaaat atataattta gttaaggaag ctcaaaatgt aggggttgag
    45481 aacatgaaag ctggtgtaca tgcgacaatt ccagatgctg agataagaaa agttgtcaaa
    45541 aaatatgaag actattacta tcaaggtata ggacatggag tcggtagaga tgttcatgaa
    45601 gaacctttca ttggtaatta tggtgataaa atcatagaag agggatgtat aataacaatg
    45661 gaaccaggta tttatttccc tggatggggt ggagttagaa tagaagatac tgttttgatt
    45721 actaaaaatg gtccagaaag acttactaaa ttccctaaag atttgatgat tttagacaaa
    45781 taatttaatt tatatatatt ttttacataa aatataacat aatctcacta ttaagctcta
    45841 tttacatatt atagtaaaga gggcttaatt ttattgtgag aggtgatagt atatatgtat
    45901 aaaaaacata aatttataaa aaatataaaa aaagcctggt ataaaatcaa attaattgat
    45961 agatgcctta taattgtaat ggcaatatta atgtttcaat ctatatataa tttatttgtt
    46021 aatgaagtaa attcacaaga tactacaact attgatgtag tagtaagaac cacttcagca
    46081 gctatatttg ggtatttttt aagtgctaat tttataaaga gacctttaag gaagtataca
    46141 aatactgatg tatcttcaaa tctttttata gataataatt tatccccaaa aggtcaagag
    46201 tctagtcaaa ataatataat gaatgtcaaa aataccattg gatttacttc agaagctgat
    46261 aattatgaaa agaaaatacc tattaataat aatgaaggtt ttgaagaagg agaaactagt
    46321 gaattgcaaa ttattatagc tactgtaatt tgtatagtag cattaacagt actttttgtt
    46381 gtaagaaatt ttacaactac aacatcagct tctttaggta ctatttcaca gatgagagat
    46441 tttgtctcag gatgcgtggg ttttttgcta ggttgtccaa gtaaacctga ttaaaaaagt
    46501 attatcaata aaaggtattt taaaaagtat cagtataata atatttagat ttaaatgtat
    46561 acaattataa ttctataaca ttctatattg tatttttata ctgatactct aaactatact
    46621 atttattaaa atattttaac attgtattta aacaaaatat tatatctgta taatataaat
    46681 ttagtttata tgaatagatg ccatataatc aataaaaagt ttcatactat gagatatgta
    46741 ttgatttctt ctccaaagaa ccccaatatt tgcagtaatt gcacgttcta atggtattga
    46801 acagagattt tctgtatttt gaaatatttc tttatacaat attgttgaag ccaagtcatt
    46861 ttctagtaaa taacgtatgg tagaaagttg atttgagtgt aaaattatat ttggcttagc
    46921 tttttcaagt ctttttgtaa taatcttata gtgataagaa ccttcagata agattacaaa
    46981 aggtacattt tttaacatat aattcgatat aatgggatgt tttgccaatt catttttggg
    47041 atttgtaact aagtataatt ctgttttata gattgtcttt gaatctatat catcataaat
    47101 atcactgtca attataccaa tacaaaaatc aagtgtagat tcttcaatca ttttagctcc
    47161 aacaaatgtt ggaacttcaa aaattttaag ctctatatct ggataaatta cattaaatct
    47221 aggaagtatt tttgaaagca aaaacgtacc taaaactgta ggaataccta ttttgagaga
    47281 agcttttctc ttttttccta aatcaagtgc gtcacattga aaatcctcaa attgttttat
    47341 aaaatgtttt gacttgtcta aaaaaatttt tccttcaggc gttaactcta ttttattacc
    47401 tattctgtaa aaaagttcaa agccaagttc tttttctaat tctttaattg ctaatgttat
    47461 ggatggttga gaaatatgaa tactttctgc agccttagtc atgtttttat atttacagac
    47521 agtttgaaaa tagtataaat agtttatatt catttggtat tagttccttt catttacata
    47581 ttagtgacaa gtatataatt aacaatagca taagttttgt atattgttgt aaaaaaatag
    47641 aatagtttta aactatcata gcataagttt ttgatatttt caatctatta agaatagtgc
    47701 tattattaaa ttaacaaaag agaatagctg gtatggttta gagtaaatca atctatatta
    47761 cagttatatc aacgaatatc aaaaaattat ctattagaaa ataaaaatta tttttacaat
    47821 taaattgtta attaaatcat aaagaaaaaa gtgatattaa caagataaaa tttttttaat
    47881 cagaaatgtt aatttaataa caaaatagag tattggggag aaaggagaaa tatatttttt
    47941 aataatattt gttaatttaa taacgaaaaa tgaaagggag tgatttagtg aagtttgaat
    48001 tgatggattt tttaatgaat ccttttgtcc ttatgtttgc tgcagtaatt acaggaattt
    48061 tatttggaaa aattaaattt gggaagttta attttggggt atcaggagca ttatttacag
    48121 gattatttat tggatggctt gcatatagct tggggaatct aataatagca aaaggggaaa
    48181 cagcagcagg atataaggca gcaactgtaa tgatggggaa tggaattatt tcttctgact
    48241 tctttgattt ctttttaata atatttgttg cagcagttgg tctattggca gcaaaagata
    48301 tgaaagctgt attaaagaag tatggagcaa gatttgtaat tttaggagtt cttataactt
    48361 ttataggtgg ctctatgact tatgcaatga ctttactatc aagtgataaa ggaagtagtg
    48421 cttatgaagt ttctggcgtt tacacaggag cattaacaag ttcgcctgga ttagctgctg
    48481 ctttagaaac agctggtaaa catgcagaag atgtatcaaa agaatttgaa aaagcttcaa
    48541 taaaagataa aaaagaaatt ttaaaagtag tcgacccaga aggcaaattg gatgtgaata
    48601 caactacatc actgactcaa gagcaaatag ataagtatat tgcatatgct gaagctggtg
    48661 ttggtatagg tcatgcagtc gcatatcctt ttggagtttt aatagtaatc ttaggtgtaa
    48721 atttccttcc aaaattattt aggatggacc taaaagaaga aagaagaaaa tatgagaagg
    48781 aaatgaagga agcaagagat agtgtttctg gtaaaacgat accagaagtt ccatttaata
    48841 taataacatt tttcttgaca tgtttagctg gatacttagt tggaggaata catgtattta
    48901 tgggaccact agggtacttc acactaggcg caacaggagg ttcactaatt gtatctctag
    48961 ttcttggata tataggtaag ataggggtag taaatttcag aatggaagaa aaggttttaa
    49021 atattttaaa acaaataggg ctagtatttt tcttagcaat agttggatta agatatggtg
    49081 gaaaagtagt tgattcaatt atgacatctg gtatgcacct agccttagta gcaatagcag
    49141 taggtgttac agctatgatg ataggattct tagttggcaa atacgtattt aaattaaatt
    49201 ggattttatt atctggagct gtctgtggtg gtatgacatc aacaccaggt cttggagctg
    49261 ctgttgatgc tcttgatagt gatgacccag cagcaggtta tggtgctacg tatccatttg
    49321 cattattaac taaagttata ttggttatag ttttacacaa acttccaatg taaataagat
    49381 tttatatggg gggatattta tgaataatga aggagcattt gaagaattaa gtggaagatt
    49441 aagaaataaa gaattgtgtg atttaataat gaatgcagat gaagctgcta aaattataga
    49501 ggatggcatg gttattggag ttagtggatt tacaccttca ggctatccaa aagcagtacc
    49561 attagcagtt tcagaaagag ctaaaagtgg agaaaagata aaattaaccg tatattcagg
    49621 agcatcttta ggcccagaag ttgatggagc atggtcagaa gcaggtataa ttgaaagaag
    49681 acttccttat caaactaatt caatacttag aaataatatt aataaaggtg ttgttgatta
    49741 tatagatatg cacctaagtc attctactca atttttaaat tatggaacta tacctaaagt
    49801 agatgtagct atagtagaag cacttgcaat aactgaagaa gggaatatta taccaactag
    49861 tgggataggt aattctccat cttttataaa aagtgcagat aaagtaatag tagagataaa
    49921 tcttgcaaaa ccaatggaaa tggaaggtat ggcagatata tatattacgg aaaatccacc
    49981 aaatagaaaa ccaatagaaa taaatcatcc taaggataga attggaacaa catatatacc
    50041 ttgtggtttg gataaaattg caggagtagt aattaccaat atgcaagata agacccgtcc
    50101 attgggggtt gtagatgaag cttcgaaaaa aatatcaaat aatattattg cttttttaag
    50161 agaagaagta aaggcaggca gattatcaaa gaatttactt cctcttcaat ctggtgtagg
    50221 aagtgtagca aatgcagtat tatatggatt atgtgaatct gaatttgaaa atttaacttg
    50281 ttatacagag gttgtacagg actctatgtt ggatttaata agaatgggta aagttacaat
    50341 ggcatctact acatctgtaa gtccatctcc agaaggatta ataaaatttg aaaaagatat
    50401 tgattttttt aaggataaga ttatattaag acctcaagaa attagtaaca atccagaaat
    50461 agccagaaga attggtgtaa ttgcaatgaa cactgcaatt gaagttgata tttatggaaa
    50521 tgtaaattct actcacataa tggggtcaaa gatgatgaat ggaattggtg gttctggtga
    50581 ttttgctaga aatggagcaa taactatatt tagtactgag tcgattgcaa aaaatggtga
    50641 tatatcatca atagtaccta tggtatctca cgtagaccac actgaacatg atgtaatggt
    50701 aattgtaaca gaacaaggat atgctgattt aagaggtctt gcacctagag aaagagctat
    50761 aaaaataata gaaaattgtg cccatccaga ttataaagaa caacttaggg attatttaaa
    50821 tagagcttgt aaaagtggag caaaacaaac acctcacata ttggacgaag ctttatcatg
    50881 gcatagtaaa tttatgagta caggtacaat gaaaaaggct gaaactttta aaagtgcatt
    50941 ataaaatatt taataaattg gaggtagatt ttaaatggaa aaagcagtgg aaaactttga
    51001 agatttaagt aaggaataca ttaatggtta tattgagaga gcaagaaaag ctcaaagaga
    51061 gtttgaatgt tatactcaag aacaagtaga taaaatagta aaaatagttg gaaaagtagt
    51121 gtattataat gctgaatatt tagctaaact tgctgtagaa gagactggaa tgggagtata
    51181 tgaagataag gtagctaaaa ataaaagcaa agcaaaagtt atatataaca atttaaaaga
    51241 taaaaaatca gttggtataa ttgatataga tagagaaact ggaataacaa aggtagctaa
    51301 accagttgga gtagtagcgg caataactcc ttgtacaaat ccaatcgtta cacctatgag
    51361 caatgcaatg tttgcactta agggaagaaa tgcaattatt atcacacctc atcataaagc
    51421 aattggatgt agtacaaaaa ctgtagagat gattaatgaa gaattagaaa aaataggagc
    51481 tccagaaaat ttaatacaaa tacttgacca acaatcaaga gaaaatacaa gaaatttaat
    51541 ttcatcagca gatgtagtaa ttgccactgg aggaatggga atggtaaaag ctgcgtactc
    51601 aagtggaaaa ccagcacttg gtgtaggggc aggaaatgtt caatgtatca tagatagaga
    51661 tgtagacatt aaagaagcag tacctaaaat catagcagga agaatttttg ataatggtat
    51721 catttgttca ggtgaacagt cagtaatagt agcagaagaa atgtttgata aaataatgga
    51781 tgaatttaaa aataataaag gttttatagt tagagataaa gtacaaaaag aagctttcag
    51841 aaatgctatg tttgtaaata aatctatgaa taaagatgca gtaggtcaat cagttcatac
    51901 aattgcaaaa attgcaggtg ttgagatacc agaagataca aaaataatag taatagaagc
    51961 tgatggacca ggagaagagg atattattgc aaaagaaaaa atgtgtccag ttatatctgc
    52021 atacaagtat aaaagttttg aagaaggtgt agctattgca aaagcaaatt taaatgtaga
    52081 aggaaaagga catagtgtat ctatacattc aaatacagtt aaaaatatag aatatgcagg
    52141 agaaaatata gaagtatcaa gatttgttat taatcaatgt tgtgctacta gtgctggagg
    52201 aagtttcttt aatggtcttg cacctactaa tactctaggt tgtggttctt ggggaaataa
    52261 cagcatctct gaaaatcttg attataagca tctaattaat atttctagaa ttgcttatta
    52321 tatgccagaa aatgaagtcc ctacagatga agaactttgg ggataaaatt tagacaaaaa
    52381 atattcattc taaattaaag taggaatata ttttaattta gaatgaattt aagacataaa
    52441 ttaaatttaa attagtttat tggactaaat ttaaaatatt aagagacata tagaagaagg
    52501 aagtattata atttaaaata aataaaaaaa tctataaaag agaaggagtg gttttattat
    52561 ggcattaatg acaggagctc aatatattga aagtttgaga aaattaaata ctaaggttta
    52621 tatgtttggt gaagaggtta agaattgggt tgaccatcct atgattagac catctataaa
    52681 ttgtgtagca gcaacttatg acttagctca tgacccagaa tatgcagatt taatgacagt
    52741 tacttcaaat atcacaggtg aaaaaataaa tagatttggt catcttcatc aaagtgttga
    52801 tgatttgata aaaaaagtaa aaatgcaaag actatgtggt caaaaaacag cttcttgttt
    52861 ccaaagatgt gttggtatgg atgcgtttaa tgcagtgtat tcaacaacat ttgagtgtga
    52921 taaagcccat ggaactaatt atcatgataa ttttgtaaaa tacttaactt atatacaaga
    52981 aaacgattta gtagttgatg gagctatgac tgaccctaag ggagatagaa gtctatcacc
    53041 tagtgctcaa cctgacccag atatgttcct tcatatagta gaaagaagag aagatgggat
    53101 aattgtaaga ggagcaaagg cacatcaaac tggttcaata aattcacatg agcaccttat
    53161 aatgcctact ataagtatga ctgaagcaga taaagactac gctgtatcat ttgcagttcc
    53221 ttcagatgct gaaggagtgt ttatgattta tggaagacaa tcttgtgaca ctagaaaatt
    53281 agaagaaggt gcagatgttg atttaggaaa taaggagttt ggaggtcaag aagctttagt
    53341 agtatttgat aatgtatttg ttccaaatga tagaattttc ttatgtggag aatgggattt
    53401 ctctggtatg ttagtagaga gatttgcagg ataccataga caaagttatg gtggatgtaa
    53461 agtaggagta ggtgatgtaa taataggagc agcagcttta gcagctgatt ataatggagc
    53521 aaacaaagct tcacatataa aagataaact aatagaaatg actcacctta atgaaagctt
    53581 atattgctgt ggtatagcat gttcatcaga aggtcacaaa acagaagctg gaaactatca
    53641 aatagattta cttcttgcaa atgtgtgtaa acaaaatgta actagatttc cttatgaaat
    53701 agtaagactt gcagaagata tagctggagg gcttatggtt actatgcctt cagaaaaaga
    53761 ctttaaatca gaccttaaag ttggaacaag tggaatgact attggcgaag tatgtaacaa
    53821 gtattttaaa gcttcttcag tggcttcaac agaagaaaga atgagaatat taagattctt
    53881 agaaaatata tgccttggtt catctgctgt tggttataga actgagtcaa tgcatggggc
    53941 aggttcacca caagctcaaa gaataatgat ttcaagacaa ggaaacataa atcaaaagaa
    54001 agaattagct aagaaaattg cgggaataaa aaaagaggaa gctttaaatt tataaaaagt
    54061 tgatatataa agggaaatta tatattaatg tgtattaatt aatgaaaaaa tatgtagtat
    54121 aagagatagt tataaaatta tggtggtttt tagctaagta aagaaatttt taataaggag
    54181 aatttacaca tgacctgtgg agttagattt tgtgggggct gtaaccctag atacgaaaga
    54241 gggaaggctc ttacaataat caaagagcac ttcaatggta gggtagattt tagtatagca
    54301 caagagaaca tcgagtatga tttgctactt ataattggag gatgcgcaac tctatgcgca
    54361 tcctatcaac aatataaagt gaaaaatgaa tgtgttaaga tgtgggattt tagccatata
    54421 gaaagagtta taaatgtgat tgaatcagca atttgtgaaa cttaaaaaag ctaaatgggg
    54481 ggatttcatt gagttggcaa gaattgtatc aaagtaaatt atgtagtgct acagaagctg
    54541 taaaacagat taaaaacggt gatactgtag tatttgcaca ttgtgtaggt gaacctcctg
    54601 cactagtaga ggctatgatt gaaaatgctg aacaatataa agatgttgag attaaacata
    54661 tggtaagctt gggtagtggt ggatatactg ctaagggaat ggaagctcat tttagagtaa
    54721 atccaatgtt tgtaagtgga aatgtaagaa aggcaattga aaatggagat ggtgatttta
    54781 cacctgcatt cttccatgaa gtaccaaagt tgcttagaga aaaaagatta aaatgtgatg
    54841 ttgttttagc acaggtaact ccaccagatg aacatgggta ttgtagctta ggaacatcag
    54901 ttgattatac ttatgaagcc attaaaactg ctagaacagt aattgttcaa gtgaatgacc
    54961 agtttcctag aacctatggt gaggtagtcc atgtatcaga gtttgactat atagtggaaa
    55021 aatcacaacc tttatttgag ctacaacctg caaagattgg agaagtagaa gaagcaattg
    55081 gtaaaaattg tgcttcatta attgaagatg gttctacttt acagctaggg attggtggga
    55141 taccagatgc agttatgtta tttttaactg ataaaaagga tttagggatt cattcagaaa
    55201 tgatttcaga tgggactttg gccctttatg aaaaaggtgt tataaatggt aaatataaaa
    55261 attttgataa agaaaaaatg acagttacct tcttaatggg tactaaaaaa ttatatgact
    55321 ttgcaaataa taaccctgca gtagaggtaa aaccagtaga ctatgtgaat catccagcaa
    55381 ttataatgaa acaacataag atggtttcta taaattctgc tatacaagtt gatttaatgg
    55441 gacaggtagt tgcagaggct atgggactta gacaatttag tggtgttgga ggtcaagttg
    55501 actttatacg tggagtgtct atgggtgaag atggaaaggc aattatagct atgccttcaa
    55561 taactacaaa aaaagatggt acagtaattt caaaaatcgt atctattgtg gatgaaggtg
    55621 cacctataac gacatcaaga aatgatgttg actatattgt aacagaatac ggtatagcag
    55681 aattaaaagg aaaatctctt agagaaagag caagaaattt aataaatata gctcatccaa
    55741 gtgtaagaga acatctagca gtagaatttg aaaagagatt taaggagaaa tattaataat
    55801 agaggggtgt taataatgga agcacttaga gtagttccaa agatatttta ctttgataca
    55861 tttaaagaat ttaatgaaga atttaaaata ggaaaaaatg atttagtaat tactaatgag
    55921 tttatatatg agccatatat gaaacctctt ggaatagata caaatttaat ttttcaagaa
    55981 aagtttggaa caggtgaacc atctgatgaa atgatagact ccatgacaaa ggaaatgaag
    56041 aaatacaatt ttgatagaat tattgcattt ggaggaggaa caatagttga tatatgtaag
    56101 atattagcac ttgatgttcc agaaaagtct attgatttat ttgaaggaga agtatcacct
    56161 aaaaaagtaa aagagttagt agtagttcct acaacatgtg gtacaggtag tgaagtaaca
    56221 aatgtagcta ttgcagagtt aaaatctaaa catactaaaa aggggcttgc agtagaagaa
    56281 acatatgcag actatgcagt gttaattcca gagacagtaa aagggttacc ttacaaattc
    56341 tttgttacaa gttcggtaga tgctcttata catgctattg agtcttatct ttctccaaaa
    56401 gcaagtccat ttacagagat gtattctcta caagcaataa aaatgattat ggatgggtat
    56461 aagaagatag ttgataaagg agaagaagag agatttaatc atcttagaga ctttgtactt
    56521 gcatcaaatt atgcaggaat tgcttttgga aatgctggtt gtgcagcagt tcatgcacta
    56581 tcttattcaa taggtggagc tttccatgtt gcacatggtg aagcaaatta tcagttcttt
    56641 actgaagtat ttaagatgta ttctagaaaa aaacctaatg gaaaaattat aaaatgtaca
    56701 aaaattttag cagatgccct tgaatgtgac cctaactgtg atgtttatgg agaattagaa
    56761 aagtttttaa ataaattaat agctaaaaaa gctttgagag aatatggaat ggtggaaagt
    56821 cagatagatg aatttacaga tagtacaata gcaaatcaac aacgactact tgctaataat
    56881 tatgtagagc ttagtagaga ggaaataaga gagatatttg caaatcttta ttaatacaat
    56941 tttttattta tatgtatagt caacataaac aatgctaatt atatttatgt acaaataaaa
    57001 ataagggaac ttaaatttta attaaagtta agttctctta tttttatatg gagttaaata
    57061 taagtatatt tttaatttaa aatatgctta tatttaagtc tattacttta tttgaaacaa
    57121 tttgtagaat ttcaaattcc ttgacagtaa taagtaagta attgtataat caaactgcaa
    57181 tatattaata atattagtga aaatgaaagg attataaaat atgtataaat cagaaaattt
    57241 ttcaaaaaaa tctgtattaa taaattcatt tttaagtaaa tttttaaaaa taattggatg
    57301 gggtttggta agcttattta cattatcagc cttagtactt tcaactattt ccattataat
    57361 tggatgggct gtaatacttc atattcaagt atgtgcagta tttttactta taggaggagt
    57421 ttgtattttt aaaggtaaga gattaggaga ccaaatttct cgttataaaa aatattgtac
    57481 aattataaac aatagaaata ttattccagt agaacttatt gcagaaaaca catctaaatc
    57541 attaaatttt ataataaaag atgtacaaaa gatgatagac aaaaactact ttataaatac
    57601 ttatattgat aaaagaaata atcagattgt gttaacaaat gaagattttg ttccaccaag
    57661 atatgaagat gtagcatatg aagtcaaaga agaagaaaag actgatgaat ccaatgaagt
    57721 tgatacaatt ataaataaag gaatgaatta tctaacacaa ataaaagaag ctaataaaaa
    57781 tattaaatca gaatcaatgg gcgataaaat tgtacaagta gaagatgtta caagtaaaat
    57841 atttgatgtt gtaaaacatg acccatcaaa acttactcaa atacaaaaat ttatggatta
    57901 ttatcttcca accactttaa aactattaaa ttcatatcat acattggaag aacaaggaat
    57961 tgatagagaa aatataacaa caactatgga aagtattgaa aatacaatga atacgattgt
    58021 agtagctttt gaaaatcaat tggactatct gtttgaagat gaagccattg atatttctac
    58081 agatattact gttttagaga atatgttagt ccaagaagga ttaacgagtg gtaaatttta
    58141 acttcaatag aaaaaatatt ctgttatttt taaacaatta attagtcata aagtataaaa
    58201 tgtgtattat ataagttaat cagtacttta tgaaggaaga ggagagaaag atacaatgaa
    58261 taatttagat gatataccag ttatgccaac tttgacactt gacccatttg gagaagaatc
    58321 aaacactaat gatatagata attctgactt attaatgaaa aaggatgaaa aagacccaga
    58381 agaagaaaaa ctgtctgaat ctgaacgcaa gatggtaaag gaatttgcgg aaaaaataga
    58441 tattacaaat acaaatatga ttttgcaata tggagctggt gctcaaaaaa aggtagcaag
    58501 tttttctgaa acagctttaa agagtgtaaa gacaagagat ttaggagaag ttggagatat
    58561 gcttacaaat ctagttactg atttaaaagc attttcagca gatgaaaaag aacaaagtgg
    58621 ttttttagga atttttaaaa aggcaaataa taaaatttca aatttaaaaa ctaagtatga
    58681 tagtgcagag gtaaatgtgg ataaagtaag caaagagctt caaaagcatc aggttaaaat
    58741 attgaaagat atagcaatgc ttgataagat gtatgaattg aacttagctt actcaaaaga
    58801 gcttactatg tatattatag caggaaaaca aaaattaaag gatatgaaag aatatgagat
    58861 gccaaagttg agagaaaaag ctcgtttatc tggttcaaca gaagatgctc aatctgtaaa
    58921 tgatatggtt tcactttgtg acagatttga aaagaagatt catgacttag aattaactag
    58981 aatggtatcg cttcaaatgg ctcctcagat aagattggtt caaaatagta ataatctgat
    59041 ggcagaaaag attcaatcaa ctatagtaaa taccattcca ttatggaaaa atcaaatagt
    59101 gcttgctctt ggaatttctc acttaaatca agctatgaaa gcacagcgtg aagttagtaa
    59161 tatgacaaat gagcttttat taaagaatgc agaaactttg aagatgggaa caatagaaac
    59221 agcaaaagaa tcagaaagag gaattgtaga tatagacaca attaagaaaa ctaatcaatc
    59281 tctaatatct actattgatg aggtagttaa aatacaacat gatggtagaa taaagagaca
    59341 agaagcagaa gttgaattat caaaaataga aaatgaactt aaaagtaagc ttcttgaatt
    59401 tacagttaaa taaaaattat actaatataa aaagagactg tctcaaaata gagataagtc
    59461 tctttttcat tgtttaaatg attaaagtat atttatatta taaatttcta ttatctttta
    59521 acataacatc atgagctcca ccttcaacta tactagtaga agatacaagt gtaagtttag
    59581 catttttttg aagttcagga atgctaagtg ctccacagtt acacatagta gaacgaactt
    59641 tacttaaaga aagagttaca ttgtctttca aagaaccagc ataaggcaca taagagtcaa
    59701 caccttcttc aaaagaaagt ttcttatctc caccaaggtc atatctttgc cagtttctag
    59761 cacgagcaga accttctccc caatactctt tcatataact accatttata ttaactttgt
    59821 tagttggact ctcatcaaaa cgagagaagt atctaccaag cattataaaa tctgcaccca
    59881 tagctaaagc taaagttata tgatggtcat aaacgatacc accatcagag caaattggaa
    59941 tataaatacc tgttttttca aagtattcgt ctctagcttg agcaacttca attatagaag
    60001 tagcttgtcc acgtcctata cctttttgtt cacgagttat acatatagaa ccaccaccaa
    60061 taccaacttt aacgaaatca gcaccagctt cagctaggta aaggaatcct tcgcggtcaa
    60121 ctacatttcc agctcctacc ttaactgtat caccatattt ttcacgaatg aaatcaagtg
    60181 ttattttttg ccactcagaa aaaccttctg aagaatctat acataaaaca tcagcaccag
    60241 cttcaactaa agcaggaaca cgttcagcaa agtctctagt attaatacca gctccaacta
    60301 catatctctt agaagaatct aaaagttcaa gtggattttc tttatgagaa gagtaatctt
    60361 tacggaatac catatacata agattaccat tatcatcaag cacaggaaga gaattaagtt
    60421 tgtggtccca tataatgttg tttgcttcct ttaaagtaac atctttgtta gcataaacta
    60481 tagatgacat aggagtcata aattcactaa ctttagtatc taaatccata cgacttatac
    60541 gataatcacg acttgcaaca atacccaata atttaccagt tgaagttcca tcttcagtaa
    60601 cagctactgt agaatgtcca gttttttctt ttaagtcaag tatgtctttt aatgtgttat
    60661 caggtttaat atttgaatca cttacaacaa aaccagcttt gtgggattta acacgtgcta
    60721 ccatagcagc ttcattttca atagtttgag aaccgtatat aaatgaaata ccaccttctt
    60781 tagcaagtgc aatagccatt ttgtcatcag aaacagattg cataatagca gaagtcaaag
    60841 gtatattcat ataaatatct gcttcctcgc cctttttaaa tttagtaaca ggtgttttta
    60901 aacttacatt agcaggtata caatcagtag aagagaaacc tggtacaaga aggtattcac
    60961 tgaaagtatg tgatggtgta tcaaaataaa acgccatttt ttaatcactc cttatatttt
    61021 tatacttttt ataattgata gtattaatta taacttattc tttaatttta ttaaagtatt
    61081 ttgtcaaata ttgagatttt tgaaaaattt aaactatttt aattaaattt gtttaatata
    61141 tattgcctta tagggtaaag ttgtatatta aataatgttt agtgtttaat agtagctaat
    61201 ttaaaattaa tctttagtga ttcatataat tacatttgta tagtttacat tgataatatt
    61261 actagagagt gtataaacct caaaaataca gataaattaa atctatatta gtatatccaa
    61321 aagtacattt aataaattta tttaactgaa ttattatttt catttctatt aaatttaaat
    61381 attttataaa aatagtatta tttacaaaat catatactcc aaaatactac ataaataaaa
    61441 ttaaacaaaa actaaaatga caaatgtcat tatctaaagt gacatagaaa cggtttctta
    61501 aagttgttac tataaaatat aaactctata ataaaagtat aacaaaaggg aatcatatcg
    61561 atatagaata taattcaaga ggaggtaaaa ttatgagtag ttctaatcaa tcgacaataa
    61621 agacatctat acagcgagta gggaaatttt taagtggtat ggtaatgcca aatataggag
    61681 cttttatagc atggggtctt ataacagcat tatttatacc aacaggatgg atgcctaatg
    61741 aaaaattgag tactataggt gacccaatga taaaatacct tttaccacta ttgatagctt
    61801 atactggtgg taaagcaata gcaggacaaa gaggtggcgt aataggtgca gcagcagcta
    61861 tgggagtaat tgttggtgca gatataccaa tgtttatcgg agcaatgata atgggaccat
    61921 ttgcaggatg ggtaattaag aagtttgata aatttgtaga tggaaagata ccaacagggt
    61981 ttgaaatgtt agtaaataac ttttcaatag gtataatagg tatgcttctt gcaatactag
    62041 gattttatgc aattggacca gcaatagtag cagggacagc tcttatagaa tctggagttc
    62101 aatttatagt tagcaaaagt ttattgcctc tagtttcagt atttatagaa cctggaaaag
    62161 tattattctt aaacaatgca ataaatcatg gtatattagg gcctataggt atagctgagg
    62221 caaaagaagc aggtaaatct ataatgttct tactagaaag taaccctgga ccaggtttag
    62281 gagttctatt agcatattgg atgtttagta gaggaagtgt aaaacaatca gcaccaggag
    62341 ctgtaataat acatttcttt ggtggtatcc atgaaattta cttcccatat attttaatga
    62401 atccagtttt aatattggct acaatggcag gtggagcagc aggaatattg actttttcaa
    62461 tattaggagc tgggcttgta gcagcaccat caccaggaag tatatttgca cttatggcac
    62521 tagctccaaa gggaggatta cttccagtat tagcaggagt tgcagtggca acagttgtat
    62581 cattcttagt ggcagcacca tttgttaaga gagcttcagc aaatcaaagt gaagaagaca
    62641 gtacttcttt agaagaagct aaagcaaaaa tgagtgatat gaaatctgca agtaagaact
    62701 ctgagaaaaa tattgaggaa aaacagcttg aagtaaatga gattaagaag attgtatttg
    62761 catgtgatgc aggaatgggt tcgagtgcaa tgggagcttc cagatttaaa aatagaataa
    62821 aaaacttaga cttaaatata gaaattacaa attcatctgt tgataatctt ccagatgata
    62881 cacaaatagt agtaactcac aatacattag ttgaaagagt tgctaagaat aattcgagtg
    62941 ttgagatagt ttcaataaac aattttttaa atgacccaaa tttagataca ttatttaaaa
    63001 gattagaatc taaataaaaa taaaaagtac cactaattag gagtggataa tttgaaaaaa
    63061 aagaagatta catctaggca aaaaaagata atactaatga tagtagaaaa ttcaaagaaa
    63121 aacattccaa taactatttc tgagattgca gggactttag agttaagttc aagaactgta
    63181 cttagagata tgtctggaat tgaaaaatgg tttgatgaaa atgattttaa ctttgtaaaa
    63241 aaaccaggag tgggattgat tttagaggaa aatatagaaa atcaaaactt tataattgag
    63301 ttgttagaag aagaaaaaat agaaaaagaa tacagcaagg aagaaagaaa tttaattata
    63361 ttatcaaagc tactagtttc taacgagcca gtaaaatcct attattttgc aaaaatatta
    63421 aaggtatctg aaggggtact gaataatgat tttgccttag caagtaaatg gttggaaaga
    63481 tttgatattg agcttgttag aaaaccaggg cttggtgtct atttaaaagg tcaagaaaaa
    63541 agctttagag aggcatatgt aaatcttata tacgattcat tcaatgaaaa agaaatacta
    63601 gatatggtca gaaatataag tgaaaatata caaacagata aagcaataga aattttaagt
    63661 gaaaatagac ttttaaattt aatggacagg tgcataataa gaaaagttga agctacatta
    63721 acaaagaagc tttcagattt agatgtaaat ttagctgata gtgcatatat agggcttgta
    63781 gttcatattt cattagcctt acagaggata aaaaatggtg aaaatataac tatggataaa
    63841 gagtttctaa aagaattatc tataacagaa gagtttaaat tagctaaaga aatcgtaaaa
    63901 ggtatggaaa cagattttaa catggatata ccagtagatg aggttggata tataactatg
    63961 catataagag gtgcaaagca aaggtcatct tcaaaccata aggctttaaa cttagatgat
    64021 atagaaataa tggaaattac aaataagatg atagatttgg cggaagatga atttaaaata
    64081 agtttaaaaa atgacgagag gttatttaaa gacctagcaa atcacttagg gccttctata
    64141 aataggttga atatgggatt agaaataaga aatcctcttt tagatgaaat aaaaagcaag
    64201 tattcatatg cctatgatgg agttgaaaag atttctagaa ttataaaaga taaactaaat
    64261 ataaattcaa tacctgaatc agaaatagga tatattgcaa tgcactttgc atcagccata
    64321 gaaaaaaatc ttatgatgaa tactaatata aatattgttg tggcatgtcc cactggaata
    64381 ggtacttcaa gattcttatc aactaagatt gaaaataaat ttcctaattt aaatatactt
    64441 gaaaccatat cagctataaa catagatgaa gagtatttaa aggaaaaaga tgttgattta
    64501 atagtgtcta cagttgagtt aaatacaagt ttaaattata tatgtgtagg tccatttatg
    64561 agtttagatg atgaacagat aatcaaggaa aaaataaaat ctatagctca aaataaatta
    64621 atcaatttaa atgcgaaaaa tgatactaag agtaagaata aagtatatga acagataaca
    64681 gaaagtatga atataggaaa agatattctt caatttttag aggaaattag atttgaaaaa
    64741 tttgaaagta aagatttaag tgagcttatt gaagactcat caaaaatatt tgcaaaatct
    64801 agtgaagata taatctctat aaaagaatcg ttaaaagaaa gattaaaaat ttcaattcca
    64861 tatatagaag aatcaaaaat tctattgtta cattgtatga gtgaaagaat agatattatg
    64921 aagttatcaa taataaaact agagaataga atcgtgctag attcaaatga agaaatcgac
    64981 aatgtagttt ttatgttact tcctaaaaat tcaccaagtt atcaaagaca aataatgagt
    65041 gaaataagtg gttcattaat agacaatata atttttacta ataagataaa taaattctct
    65101 atagaagaga tggcattaga ggtaaaagat atagttttta atttttatac aaacagatta
    65161 aaagcattta tagataaata aaattaatat taaaaaggaa ggttgatact atgagtaaag
    65221 tattgaatga gaataatata tttttagggt tagatagtgt ttcaaaggaa gaagctataa
    65281 ctttagcagg aagaaaatta gttgaaaatg gatatgtaaa agaagaatat ataccagcaa
    65341 tgttagaaag agaaaaagta atgactacat atatgggaat gggagttgct attccacatg
    65401 gtgtaaatga agcaaaaaaa gaaatattat cttctggaat agttatatta caattcccta
    65461 atggtataga ttttgatgga gaaaaagcat atttattaat aggaatagct ggtgttggag
    65521 atgaacattt agaaatacta tctaatatag cagtagtttt agatgatgac ttaacagaaa
    65581 gactaaaaaa ttcaaatgac aaacaagctt ttatggaagc ttttgccaac taatcttagg
    65641 agggatttaa aatgaaaaag gcaattcagt ttggagcagg aaatattgga aggggtttta
    65701 ttggaggctt gttagttaag tcgggttatc atgtagtatt tgctgatgta aatgaggata
    65761 tattaaactc aataaacaaa gataaaaagt atactataca cataagagat gtagaatgta
    65821 tagatgaagt aattgataat ataagtgcgg tttcatctat aaaagaagaa ataatagatg
    65881 agattgtaca ggctgaaata ataactacag cagttggacc tttagttttg actaaaatag
    65941 cctctacaat tgcaaaaggt ataaaggcta gaaaagaaaa gggattaaca agtaatctaa
    66001 atataatagc atgtgaaaat gcaatatacg caagttcttc tttaaaagaa gaggtactta
    66061 agtatttaaa taaggaagaa gtagaatatt tagagatgta tgttggtttt ccaaactgtt
    66121 cagttgatag aatagttcct cctggtaaaa acgaaaatcc tcttgatgta acagttgaaa
    66181 atttttatga gtggaatgtt gaaaaacaag gatttaaagg ggaaatacca actatagttg
    66241 gaatgaattt agctgataat cttatggctt atatagaacg taaattgttt acactgaata
    66301 caggtcatgc tataacagct tatataggat atttaaaagg atataagact atagaagaga
    66361 gtataaaaga taaatttatc tgtgatatag tcaaaagtgc aatggtagaa agtggagaag
    66421 gattgattaa aaaatacaat tttgactcag aagtgcacta taaatatatt gataaaattt
    66481 taaatagatt taaaaatcca tatcttaatg atgatgtatt aagagtcgga agggaaccat
    66541 taagaaaact aagtgataaa gatagattga ttaaaccttt aatgacagca aaatcatatg
    66601 gattatcagt tgataatttg attcttggaa taggtgcagc gcttcattat aataatagtg
    66661 aagatactca aagtgtagaa ttacaagagt tgataaagtc tattggggta aaaaaagccg
    66721 ttgctaaaat agctaatata agcaatgacg aagaactttt aaataatatc gaaaaaagtt
    66781 atatatttat gaaaaattta taaatttact tgaaaaaaag tagttctgat aaagtaacta
    66841 taatatatta ttctacacag tttgaattat ttgttgaata atagagaatt ataatatata
    66901 attaaaagga ataaattaat atatttacct gtatatgttt gataatatgg ttcaaatgtt
    66961 tctactaagc taccattaat agcttgacta caggagtgaa gaagatatag tttatactta
    67021 ttttaaatta tatattaagt taattacttt gaattttaag ctcctgtcta tgatatagac
    67081 aggagctttt ttagtatttt aatataaaat acttacaaga atagatagca aaattagaac
    67141 aaatataatt tttttattaa tataatttta gaaagggcta ggtggaagtt caatgaaggc
    67201 tttacaagaa aagatattga gagaaggcag tgtttcagga aatgatatac taaaagttga
    67261 tagtttttta aaccatcaaa ttgatgtagc ttttttaaat gaaataggaa gagaatttaa
    67321 agagagattt aaaggagaaa aggtggataa gatatttaca atagaggctt caggaatagc
    67381 tatagcatca attgtttcac agtattttga taatgcacct gtagtatttg caaaaaaatc
    67441 agaatctaaa aatttggata aagatgtata tgaaactaat gtatattcat ttactaaggc
    67501 aagagagtat tcagttaaag tttctaaaaa gtacataaac aaaggcgaaa atatattgat
    67561 agtggatgat tttttggcaa atggaagagc tgctcttgga cttaaagatt taattgagca
    67621 agcagaagct aacttagttg gagtaggaat tgtcattgaa aaaggatttc aagctggagg
    67681 agcactttta aaggctaatg atgtaagatt agagtctttg gcagtagttg aatctataga
    67741 taatggaact gtaaaattta ggtaatttat gatattatat acttgtgttt agcacatatt
    67801 ttaactatgt aattatggag gattttttat gagatttttt atagacacag ccaatattga
    67861 agaaataaaa gaagcaaatg atttaggtgt aatctgtggt gtaactacta atccgtctct
    67921 aatagctaaa gaaggtagag attttataga ggtagttaaa gaaataagtg aaatagtaga
    67981 tggaccaata agtgcagaag taataagttt agaacataaa ggtatgatag aggaagcaga
    68041 aaaattatct aagatacata aaaatatagt tataaagata cctatgacag cagaaggtct
    68101 aaaagcggtg aaagtactat caagtaaagg tataaagact aatgtaacgc ttatattctc
    68161 tgctggacaa gctcttttag ctgctagagc tggagctaca tatgtaagtc cttttgttgg
    68221 tagattggat gatatatcac aaaatggtct tgatttaatt gaagaaatcg ttgatatatt
    68281 tagtgtaaat agtattgagg ctcaaataat agttgctagt gttagaaatc caatacatgt
    68341 tttacaagct gctagaatgg gagcagatat agcaacagtt cctcttaaag ttataaacca
    68401 aatgattaag catcctttga cagacaaagg aatagatagc tttatgaagg attgggaagg
    68461 tgcttcttta aaattataat tataaactac gctttataag tatattaaag aggttattat
    68521 ggaaattcta agtaataagg ctaggctatt ttgcctagtc ttattttggt gtataatata
    68581 ttgaaacata ttattgaaat agattaattt tttaagtgag gaggcgaaaa atggataaaa
    68641 tattaagaga gatacaaaga gaagataaaa aaaatccata tactgaccaa gaacttgctg
    68701 aaatgttaaa tgtagctagg tctgaagtga tatcagttag gaaaaaaaat aatattttag
    68761 attcgagaga aagaagaaaa aaaatattaa taaaagatat atcaaaaatt gtaagtgaaa
    68821 atcctcaaat gtctgaaaga aaaataactg aaatattaat agaaaatgga tataatatat
    68881 cgagaagtgc agtttcaaaa ttattaaaag aggaaaattt aaatcagtca aagattggga
    68941 ttaaggataa agacccagca tcatcaggaa agtatgtgaa ggtgagtaaa gttgtagaag
    69001 aagatggatt tgaagaattg ataggtataa agggaagttt gaaagaaaag gtaaatttag
    69061 ctaagtcagc aataatgtat cctcctaatg gccttcatac tataatatat ggagaaacag
    69121 gagtaggtaa aagtgaatta gctacatgta tgtataaata tgctataaaa aataacatta
    69181 aagaagagaa cagcccattt atagtgttta actgtgcaga ctatgcagaa aatcctaatc
    69241 tattgatagc tcaactcttt ggggtagtaa aaggtgcata tacaggagca gatgcaaata
    69301 gagaaggtct tgtagaacaa gctaataatg gaatattgtt tttagatgaa attcatagat
    69361 tgcctgctgc tgggcaagaa atcttatttt cattaataga tagaggagaa tttagaaggt
    69421 taggagaaag tagttctaat agaaaggcaa atgttctcat aataagtgct acaacagaaa
    69481 atccagattc taatttactt caaacattta gaagacgtat accaatggta ataaatttac
    69541 ctaatatgag tgaaagacca aaatctgaaa gatatgaaat aataataaag ttttttgaaa
    69601 gagaagcaaa gcgagtaaac aagaatttta taataactaa agaagttatg tgtgcactta
    69661 tgaattataa gtgtactgga aatataggtc agttaaagag tgatatacaa gtaacttgtg
    69721 ctagagcttt tagtaaatcg atattttcta gcgataaggt aattatagat ttagatagtt
    69781 taagggatta tataaaaagt ggatattatg attgtaattg taaaaatgaa aagtattctg
    69841 agttttcagt tgaggatata tatatagata ttagtgaatt ggtgagtgga aaaaatagaa
    69901 ttttatctga taatgagata agtgagatat ataattatgc agaaaaagag cttaaggttt
    69961 tggaaagtaa gtgcttttca gaagaagagt taaaaaatgc atttatagaa aagttagata
    70021 agaagtttaa tgctattaaa aacaataaaa atttatctga tagaagaagt cgaatagact
    70081 ggggaggaca attaaaagag tctacactgg agataatgga taaggtaatt gtatttatta
    70141 aggagcagtt taagcatgta aatcaaggtc tgtacttggc tctagcaatt catattgagc
    70201 atgctatagg taggataaaa gatggaaaaa ctataataaa tccgagttta gataaaataa
    70261 aagcatcaat gccaatggaa tatgagttgt caagatatat aatgggtata gtagaagatt
    70321 tgacacaagt aacatttcca gaagatgagc ttggatattt ggcttattat ataaataaat
    70381 tttgttataa tgaggaaagt ataaaagata aagttaaagt agtaatagtt acgcatggta
    70441 aagttggaat tgaaatgtct aaagttgtaa atcatatttt aggtatagaa tgtacactag
    70501 gtatagaaat agcacttaca gattcaccat cagaggggat agagcatgta ttagaagagc
    70561 ttcaaaagat agaagctaga aaaggtattc tagttctaat tgatatgggt tctttggtaa
    70621 ttcttggtga tgaggttgaa aaaaggcttg gaattagatg taaaactgta aatagagtag
    70681 atacattatt ggcaatggaa gcaggaaaac ttgcaactat tgaaggaaaa agtttagatg
    70741 gtataatagc tgatttaaag aaaaataaga attatgctat gataaataca aataaattta
    70801 gttacagaaa aaacgagtat ggaaagaaaa atgtaatcat tactttatgt ttgagtggtt
    70861 taggtacagc tttaaattta aaagagcata tagaaaaaca aattaaggaa tatgacactt
    70921 taattgaagt aaagccagtt gcttttttaa ataataattt agaagaagag ttaaatgata
    70981 ttgaagctga atataatatt attgctatct gtggaactat tgatatagat tataaaaacg
    71041 tgccttttat ttcttataat gaggtacttg gtaaaaatgg tattaataaa gtattagagc
    71101 atttaaatag taaacaacca gctttaaata ttgaaaacca gttgaataat ttaatacatg
    71161 aagatttgat tttatacaat tttgaaggaa tttcaaagga gtatataatt gatactcttg
    71221 tttcaaaact tgaagaaggt ggatatgttg actcaaagta tatattaagt gtatataaaa
    71281 gagaagcaat gggaagtgtt gtaatggcct ctaaggtagc cgtgccacat ggacttccag
    71341 aaaacgttat aaagcctgca atagcaatcg cgaggttgaa taaacctata gtatgggaca
    71401 ataaatttat ggtggatttg gtggtactac tggcacttaa agaaaataat aagaaagaaa
    71461 tacgaagctt attttcaaag attaatgacc agcatacttt agaagtttta ttaaatactg
    71521 aagataaaaa tgaaataaaa gaacttttaa gctaattatt agtaccgaga agaaagacaa
    71581 aaaaatttgc tttagtataa aatttaaaac tactcaattg ctaagtagtt aattactcta
    71641 tataaggcta gttctatgaa atttatttat atgattttat agaattagct tttttcttgg
    71701 taaataaaat atatgaattt atttatgtaa aaagtaaatt ttattggaca attctatgag
    71761 aatttttcct a
//
LOCUS       DAMJYC010000009        68729 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJYC010000009 DAMJYC010000000
VERSION     DAMJYC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.009"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            80..379
                     /locus_tag="PC472_000632"
     CDS             80..379
                     /locus_tag="PC472_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2725992.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            703..2358
                     /gene="ilvD"
                     /locus_tag="PC472_000633"
     CDS             703..2358
                     /gene="ilvD"
                     /locus_tag="PC472_000633"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2725993.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2702..3331
                     /locus_tag="PC472_000634"
     CDS             2702..3331
                     /locus_tag="PC472_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2725994.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFSLIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3357..4169
                     /locus_tag="PC472_000635"
     CDS             3357..4169
                     /locus_tag="PC472_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2725995.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            4717..5808
                     /locus_tag="PC472_000636"
                     /pseudo
     CDS             4717..5808
                     /locus_tag="PC472_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
     gene            5807..6218
                     /locus_tag="PC472_000637"
                     /pseudo
     CDS             5807..6218
                     /locus_tag="PC472_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6355..7008
                     /locus_tag="PC472_000638"
     CDS             6355..7008
                     /locus_tag="PC472_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2725996.1"
                     /translation="MNNIYSDIYEKLSTLQWIMKRHQMFCQAESGPFADTSRGQGRIL
                     AMLKIQPKIATKELAYLLGIRQQSLNELLNKMEKNGYVERKPSENDKRVMIVHLTEKG
                     KQVQQPKTDYQNIFNCLLPEELLQMSQYLDRIIEAFQLQNGNALEENNMIDWMAQARE
                     RMGDEHFEQLMSMRERAFGHMRPPKDIPGAERFSENYNGYVPDRDGFQPRNFKPDIK"
     gene            7097..8419
                     /locus_tag="PC472_000639"
     CDS             7097..8419
                     /locus_tag="PC472_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2725997.1"
                     /translation="MNQKNENYWLLDAPVTKAIWHMAIPMMLGMSINIIYNITDTFFI
                     GRLNDTAALATISLLLPFTTILMAIGNLFGTGGSTLFSRLLGSENTDRTKQCSATTLW
                     LSFLFGLLTAIISIIFSNYIIRLLGADSNTFAYVKQYLIFYGMGAPFIIANFTLEQLI
                     RGDGKSVESMIGMMISIGANIILDPILMFGLQLGIRGAAIATVIGNAFAVIYYIVCIQ
                     RADNQLSALPKYFRLEKQMLKEIFLVGLSAMLLDILLIVSSLMFNYYALKYGDYVLAG
                     FGISQKLVQIVDLIGMGLYMGVIPLIATAYGARNELRVKEIIKKTALYLALVITCLFT
                     ILFTCRNFIVHCFSNDSDVIRIGAYILTVQLCSSFFAAGAGLLTGIFQAKGEGTPAVV
                     MSVMRGIMLIPAIIFGNYLFKMNGVIFSLLVAEAISCITGLVLYKLKK"
     gene            8532..8743
                     /locus_tag="PC472_000640"
                     /pseudo
     CDS             8532..8743
                     /locus_tag="PC472_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8841..9200)
                     /locus_tag="PC472_000641"
     CDS             complement(8841..9200)
                     /locus_tag="PC472_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2725998.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFHLFHDLVTMFNISVDEYFYPAKNVEKSTVSRQIDSSLDLLSDDELK
                     IIQGTIDGILNSRENKK"
     gene            9524..9898
                     /locus_tag="PC472_000642"
     CDS             9524..9898
                     /locus_tag="PC472_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HDF2725999.1"
                     /translation="MAKTIFEELGGKYERQGDYLIPCLTVPAEEEQPIGTWGQRHLDY
                     LKQYRKVTYTNLLTSGRLNTYLADIDRQAQERFERLIEGMKQAQGITERLKEENALEW
                     TGRMNNIRACAREIVNEEIIFA"
     gene            10142..11065
                     /locus_tag="PC472_000643"
     CDS             10142..11065
                     /locus_tag="PC472_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726000.1"
                     /translation="MKNVYKLEFHSGSKEELLPDFESEFPYIASNAELDKYIGRSAPW
                     HWHRAIELFYMESGTLEYYTPKKKLSFSAGSGGFINSNVLHMTKAVSRTEKNIQRLHI
                     FDTALISGNYGSKIEKKYVMPITANPQIEIYAFRPDNFIHADILDLIKQTFTLCENDI
                     GYEIKIRESLSRIWLKLFELFSASFDGKGKYDKNDERLKKMLIYIHEHFADKIAVSEL
                     ATVACLSERECFRLFQSYLHTTPTKYIQSYRLQEACEMLINGQDTITDIGHACGLGNS
                     SYFSKIFREYQNCTPIEYRKKWQDSHILCPK"
     gene            11141..11731
                     /locus_tag="PC472_000644"
     CDS             11141..11731
                     /locus_tag="PC472_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2726001.1"
                     /translation="MFYEAKDFLETADKETLHIISNARKLTREYYFTDYDNTKRKNEI
                     LKELLGAVGKNVAIDVPLHCDYGKNVFLGDDVVINMNCTFVDNKPIRIGSRVLIASNV
                     QFYTSSHPVLPQERFVSDWEETGTTFFRTYARSIEIQDNVWIGGGSIILAGVTIGENS
                     VVGAGSVVTRSIPANCVAVGNPCKVIRCFTEKRGKA"
     gene            11731..12351
                     /locus_tag="PC472_000645"
     CDS             11731..12351
                     /locus_tag="PC472_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016304801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2726002.1"
                     /translation="MKYQHIVFDIDGTLINTEHAILHSLQDTLEECIHEKVPVKQLLF
                     VMGITGEDALRQLKINNIPLSLKLWEENIKKYSNTITVYDGIQELLNSLSECDCKIGI
                     VTSKTKAEFKDDFDRFGLKHYFKTIICADDTTGHKPSTEPLLKYIEISGAYNEQILYI
                     GDSKYDSLCAKNAEVDFALAMWGSHTRELEADYFIETPNDLLGAIK"
     gene            12393..12590
                     /locus_tag="PC472_000646"
     CDS             12393..12590
                     /locus_tag="PC472_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2726003.1"
                     /translation="MNIEKWILCPVCGGKTRNKIREDTVLINYPLYCPKCRQESLIVA
                     KNMQISIVIEPNAHTYKAVNY"
     gene            12622..13242
                     /locus_tag="PC472_000647"
     CDS             12622..13242
                     /locus_tag="PC472_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2726004.1"
                     /translation="MDNNEKWLQWAVELQSLAQAGIFYGKDVFDKERYERIREIAAEM
                     ISYKSEISMDKVKNLFCNEIGYQTPKIDCRAAVFQNNKILLVQEKNGTWSLPGGWVDV
                     NVSVKENVMKEVKEEAGLDVMADMVIAVQDREKHNLPVYAYKVCKIFILCSVIGGEFK
                     SNIETISSNYFGMNELPILATEKNNEEQIKMCFEAYQSENWKTFFD"
     gene            13824..14291
                     /locus_tag="PC472_000648"
     CDS             13824..14291
                     /locus_tag="PC472_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726005.1"
                     /translation="MELSYTDFCRRRQADQSYMDKLFIPVQGCLLEVVREQYADFYKE
                     KERWRYLKKLDTNHKLLSLEGFTDSEGNVLDFVIDETVDVAETVVHAVMVDSLKAALS
                     LLSGREQALINAIFFEELSEREVGLRLGVTQSVVNKRKSKILAKLRKIIENKI"
     gene            14380..14805
                     /locus_tag="PC472_000649"
     CDS             14380..14805
                     /locus_tag="PC472_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726006.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKILRECGVDEETIEQIRTDDRADF
                     NSNRRFYRWASDFGEYLEGMADREKQAEVKSVSDLLDEIEDETLYLALVTVDRRTLQI
                     VLLKMQGYSTKEIAPLVHLTAGAVYARLNHLRKKLRKIL"
     gene            14888..15361
                     /locus_tag="PC472_000650"
     CDS             14888..15361
                     /locus_tag="PC472_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726007.1"
                     /translation="MAYGAKTYKLREESTESGARYFISFKDGQREHHELEVSEQLFIE
                     FRQMERRNRNLQQWNQRHREFNEVWDETLYRRALRVPKTLDERMIEKERNEMFLKAVA
                     RLPEIQRRRFLLYYEYDFNFYQIGAMEHCTASAVQKSVSVAREKVKAEMRKYLQS"
     gene            15512..16543
                     /locus_tag="PC472_000651"
     CDS             15512..16543
                     /locus_tag="PC472_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2726008.1"
                     /translation="MQKLQTVNADTLLYEPLEKPSFVVDGLIPTGLTLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDMPTREGDVLYLCLEDTFCRIQDRLFRLTDEASGRLYFAVA
                     SDKLSDGLIVQLEDYLKEYPDSRLIVIDTLQKVRTASKDNAYASDYGDISLIKDFADR
                     HSLAVIVVHHIRKQNDSDVFNKVSGTTGLTGSADATFVLEQESRASNAAKLYVTGRDT
                     PYQEFTLRFRDCSWELVERKEQEQLAKEAIPDVLFRLVDFMQDKEEWTGTATELLAAM
                     GETETVPTVITKWLNEYRTTFLSENHICYQYSRKKDGRQIALARQTGDSGDSDDSGIG
                     IPPVTVIDT"
     gene            17067..18032
                     /locus_tag="PC472_000652"
     CDS             17067..18032
                     /locus_tag="PC472_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDF2726009.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLIAPPKYTYKKEINRMVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKEYFQ
                     TALDFISERVGKKNILSAVVHMDERTPHMHLCFVPLTPDNKLSAKSILGNQKSLSEWQ
                     TAYHERMFARWSELERGQSSMETKRKHIPTWLYKLGGRMDKQYEEIVSALSDINAFNA
                     GKKRDKALELLSAWLPDVEKFSKEISKQSAYIDSLKERIGQESDYAGRMRDEKYEQER
                     KVQKANQRIFELQKTNQQMERLLKKIPPEVLEELQKSNRNRARER"
     gene            18041..18895
                     /locus_tag="PC472_000653"
     CDS             18041..18895
                     /locus_tag="PC472_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2726010.1"
                     /translation="MKKQDFKVLKTADLYPFPDNPFHVVEDEMLSELAESIKEFGIVT
                     PIITRPKEDGNGYEIIAGQRRVRASELAGINTIPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYKMKSEAMKRQGFRTDLTSSQVGTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKGEQENLFATMESEEATPSLSQAQRMKSLS
                     QSRRLNMDAIFAIMTEEKGNQKETVKIGMERLKKYFPKGTTPKQMEDTIIKLLERELQ
                     RKRGRDSR"
     gene            18974..19204
                     /locus_tag="PC472_000654"
     CDS             18974..19204
                     /locus_tag="PC472_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726011.1"
                     /translation="MKEIQYEIVKEIAVLSTSESGYTKEINLISWNGKEPKYDIRSFS
                     PNREKCGKGITLTADEAAALLKALQEELNSGD"
     gene            19276..19467
                     /locus_tag="PC472_000655"
     CDS             19276..19467
                     /locus_tag="PC472_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2726012.1"
                     /translation="MGEDKKANKKRKRIVARQPVQMIVSREYVGTQTVAEAFIPIISE
                     DIRRKIAEGDTFDNEDISA"
     gene            19522..20406
                     /locus_tag="PC472_000656"
                     /pseudo
     CDS             19522..20406
                     /locus_tag="PC472_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            20707..23751
                     /locus_tag="PC472_000657"
     CDS             20707..23751
                     /locus_tag="PC472_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2726013.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     gene            23984..24310
                     /locus_tag="PC472_000658"
     CDS             23984..24310
                     /locus_tag="PC472_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2726014.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            24326..24709
                     /locus_tag="PC472_000659"
     CDS             24326..24709
                     /locus_tag="PC472_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2726015.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            24877..25284
                     /locus_tag="PC472_000660"
     CDS             24877..25284
                     /locus_tag="PC472_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726016.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            25300..25626
                     /locus_tag="PC472_000661"
     CDS             25300..25626
                     /locus_tag="PC472_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726017.1"
                     /translation="MNLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGIGLLFLAIGVIGI
                     ICATLI"
     gene            25727..27121
                     /locus_tag="PC472_000662"
     CDS             25727..27121
                     /locus_tag="PC472_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726018.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            27305..28501
                     /locus_tag="PC472_000663"
     CDS             27305..28501
                     /locus_tag="PC472_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2726019.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            28514..28648
                     /locus_tag="PC472_000664"
     CDS             28514..28648
                     /locus_tag="PC472_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2726020.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            28649..28870
                     /locus_tag="PC472_000665"
     CDS             28649..28870
                     /locus_tag="PC472_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726021.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            29074..29850
                     /locus_tag="PC472_000666"
     CDS             29074..29850
                     /locus_tag="PC472_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726022.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            29935..30231
                     /locus_tag="PC472_000667"
     CDS             29935..30231
                     /locus_tag="PC472_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726023.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            30150..30653
                     /locus_tag="PC472_000668"
     CDS             30150..30653
                     /locus_tag="PC472_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2726024.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            30671..31174
                     /locus_tag="PC472_000669"
     CDS             30671..31174
                     /locus_tag="PC472_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2726025.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            31292..31690
                     /locus_tag="PC472_000670"
     CDS             31292..31690
                     /locus_tag="PC472_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726026.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            31668..34118
                     /locus_tag="PC472_000671"
     CDS             31668..34118
                     /locus_tag="PC472_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726027.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            34118..36313
                     /locus_tag="PC472_000672"
     CDS             34118..36313
                     /locus_tag="PC472_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2726028.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            36310..37317
                     /locus_tag="PC472_000673"
     CDS             36310..37317
                     /locus_tag="PC472_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2726029.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            37333..38244
                     /locus_tag="PC472_000674"
     CDS             37333..38244
                     /locus_tag="PC472_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726030.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            38365..38742
                     /locus_tag="PC472_000675"
     CDS             38365..38742
                     /locus_tag="PC472_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2726031.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            38743..39606
                     /locus_tag="PC472_000676"
     CDS             38743..39606
                     /locus_tag="PC472_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726032.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            39603..40247
                     /locus_tag="PC472_000677"
     CDS             39603..40247
                     /locus_tag="PC472_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2726033.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            40260..40451
                     /locus_tag="PC472_000678"
     CDS             40260..40451
                     /locus_tag="PC472_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HDF2726034.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            40656..40844
                     /locus_tag="PC472_000679"
     CDS             40656..40844
                     /locus_tag="PC472_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2726035.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(40952..41311)
                     /locus_tag="PC472_000680"
     CDS             complement(40952..41311)
                     /locus_tag="PC472_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726036.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            41498..41713
                     /locus_tag="PC472_000681"
     CDS             41498..41713
                     /locus_tag="PC472_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDF2726037.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            41730..42002
                     /locus_tag="PC472_000682"
     CDS             41730..42002
                     /locus_tag="PC472_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDF2726038.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            42004..42153
                     /locus_tag="PC472_000683"
                     /pseudo
     CDS             42004..42153
                     /locus_tag="PC472_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            42214..42555
                     /locus_tag="PC472_000684"
                     /pseudo
     CDS             42214..42555
                     /locus_tag="PC472_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            42680..43417
                     /gene="erm(B)"
                     /locus_tag="PC472_000685"
     CDS             42680..43417
                     /gene="erm(B)"
                     /locus_tag="PC472_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDF2726039.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            43563..43703
                     /locus_tag="PC472_000686"
                     /pseudo
     CDS             43563..43703
                     /locus_tag="PC472_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            43722..44630
                     /locus_tag="PC472_000687"
     CDS             43722..44630
                     /locus_tag="PC472_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDF2726040.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            44627..45169
                     /gene="sat4"
                     /locus_tag="PC472_000688"
     CDS             44627..45169
                     /gene="sat4"
                     /locus_tag="PC472_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDF2726041.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            45274..45606
                     /locus_tag="PC472_000689"
     CDS             45274..45606
                     /locus_tag="PC472_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2726042.1"
                     /translation="MARKYDHEYKVQAVKLAKEIGGAKAAKELGIPEGTIHTWLKAVR
                     AGTLDIGDGAHTPESAMSLAEELAMLRKRVKDQDKEIRRLKEENEFLEEASAFFAASR
                     RKSARTKE"
     gene            45603..46511
                     /locus_tag="PC472_000690"
     CDS             45603..46511
                     /locus_tag="PC472_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2726043.1"
                     /translation="MKFIAIKTEDGVIKGKLSFYCRMLGVSRQGFYKYLAIKDRPWKY
                     QDLADAMRAINSEDECNDTYGRIRMYQALLLKNLEGLKIPSERTVYRVMDEIGLSHRP
                     KRKPNGITRADREARKSDDLLKRDFKSNKPLEKCVTDITEIKAKDGKLYVSAIFDCFD
                     SSVLGLAMETNMKATLCEHTLDNAYLAYPNLRGAILHSDRGTQYTSETYRKALAKYGI
                     IQSMNSAGGRCHDNARCESMWARMKSELLYDRYNTETMTTEELKVLIWRYFISYWNNR
                     RICSANGGLPPIIKRQRYYQSLDLAA"
     gene            46629..47423
                     /locus_tag="PC472_000691"
                     /note="aph(3')-IIIa"
     CDS             46629..47423
                     /locus_tag="PC472_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDF2726044.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            47699..48271
                     /locus_tag="PC472_000692"
     CDS             47699..48271
                     /locus_tag="PC472_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2726045.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            48244..48651
                     /locus_tag="PC472_000693"
     CDS             48244..48651
                     /locus_tag="PC472_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2726046.1"
                     /translation="MVLNRFCRLRNEYRNFRVDRIKSICIEEELCQSHDGSLEQILKQ
                     MLSYKKLYNVILRAEKGETYNSIKNRYSLGFLEETDLGSKMEIEFQTDSFEILSKQLI
                     EYGSGIEIVQPDELKCITRKHLAQITNHCLNLI"
     gene            48762..49421
                     /locus_tag="PC472_000694"
     CDS             48762..49421
                     /locus_tag="PC472_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HDF2726047.1"
                     /translation="MKPKIIMHTQISLDGRIKGFDNPEVYYQVAGGIHSDAVLFGSNT
                     VFTAFEKYPAETEADFEKIITSPEDPRPIGVIPDSRGILRSLHCLRNLGYLKEIVILV
                     STATPKEYLNYLEERHYPYIVSGNDHVDYEKAFQILHEQYGCKYMRTDSGGGLTNKLL
                     ENGLIDEISLVISPCFVGNKEKQLFDNLLLSEKVNLELQGTENAEHGCLSLRYAVKNK
                     D"
     gene            complement(50142..51746)
                     /locus_tag="PC472_000695"
     CDS             complement(50142..51746)
                     /locus_tag="PC472_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
                     /protein_id="HDF2726048.1"
                     /translation="MYQNYTINQLCLPIDLEIKLEENDFAHAIVQFVDSIPDEVFLPY
                     YQSMGRPQYHPRMLLSIILCAYIQGVYSGRQIQNMLIDSIRMRYLSQEQFPNFRTINR
                     FRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIEADANKYSFVWKKSILKY
                     KDSLDQKALENYQQMVETKILPELIDDLKDELSIEEIERIRQSLEEKENQLIEAIDQT
                     ETVEERKLLRKEKSEVHKQKKRFDDFAQRKMSYEEQLVIMGVRNSFSKTDHDATFMRM
                     KEDHMLNGQLKPGYNIQLATENQFALAYDCYPNPTDTRTFIPFLEKIESKIGLPENIV
                     ADAGYASEENYCYVLDETESTPIIPHQGYLKEKKRAHKQNQFHRDNWTYNELDDYYIC
                     PNQKRVVFSHYSQRKDRNGFIRNFKIYECEDCTGCPLREKCTKAKEGKNRRLNINPTW
                     EYIKIKANEKIESKETASYYAQRKIDVETVFGNIKQNMKFKRFHVRGAEKIFKEMGLV
                     FLAHNFRKLVTRVRKYEGKTIIQNQI"
     gene            51979..52857
                     /locus_tag="PC472_000696"
     CDS             51979..52857
                     /locus_tag="PC472_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2726049.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            53305..53721
                     /locus_tag="PC472_000697"
     CDS             53305..53721
                     /locus_tag="PC472_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726050.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            53728..53976
                     /locus_tag="PC472_000698"
     CDS             53728..53976
                     /locus_tag="PC472_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726051.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            54227..54628
                     /locus_tag="PC472_000699"
     CDS             54227..54628
                     /locus_tag="PC472_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726052.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            54647..56092
                     /locus_tag="PC472_000700"
     CDS             54647..56092
                     /locus_tag="PC472_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HDF2726053.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(56301..56377)
                     /locus_tag="PC472_000701"
     tRNA            complement(56301..56377)
                     /locus_tag="PC472_000701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56341..56343),aa:Arg,seq:cct)
     gene            56447..57424
                     /locus_tag="PC472_000702"
                     /pseudo
     CDS             56447..57424
                     /locus_tag="PC472_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
     gene            58041..58979
                     /locus_tag="PC472_000703"
     CDS             58041..58979
                     /locus_tag="PC472_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2726054.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            59455..59883
                     /locus_tag="PC472_000704"
     CDS             59455..59883
                     /locus_tag="PC472_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2726055.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGDMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(60036..60242)
                     /locus_tag="PC472_000705"
     CDS             complement(60036..60242)
                     /locus_tag="PC472_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726056.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            60564..61220
                     /locus_tag="PC472_000706"
     CDS             60564..61220
                     /locus_tag="PC472_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2726057.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTIRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            61304..62383
                     /locus_tag="PC472_000707"
     CDS             61304..62383
                     /locus_tag="PC472_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2726058.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQQFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAVAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLSELKNTTIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCILLEEIAPKLAK"
     gene            63655..65310
                     /locus_tag="PC472_000708"
     CDS             63655..65310
                     /locus_tag="PC472_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726059.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            65369..66121
                     /locus_tag="PC472_000709"
     CDS             65369..66121
                     /locus_tag="PC472_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2726060.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(66637..66696)
                     /locus_tag="PC472_000710"
     CDS             complement(66637..66696)
                     /locus_tag="PC472_000710"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2726061.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            67089..67382
                     /locus_tag="PC472_000711"
     CDS             67089..67382
                     /locus_tag="PC472_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2726062.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            67363..68004
                     /locus_tag="PC472_000712"
     CDS             67363..68004
                     /locus_tag="PC472_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2726063.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            68248..68580
                     /locus_tag="PC472_000713"
     CDS             68248..68580
                     /locus_tag="PC472_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2726064.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT        23352 a        11134 c        14705 g        19538 t
ORIGIN      
        1 aaaaatttta tcgtatgcat aaatattata aatatatgga aataataata gtatattttg
       61 gtaaagtagg aggaatttta tgaaatggat taaatggtat agtattacat gcatatgtat
      121 ttttatagta gtagcttttt atatgtttat atttcctaat aaaatagaaa ctattgatac
      181 ttcatctgca tatagctttg ttgaaaagaa agtaccaaat agtgctgtgt atcaaggata
      241 caaaaagaat ccagttgatg gtactactac aatttattac agctatgata attcaactca
      301 tatagttaga ttatcacatc cagaagacta ttcaaggaag attaattggg ataaagtttc
      361 aaacatcaga tttgattaat ctttaatatt tataaaagtc tatatatcta aggcaaatat
      421 attattaatc ttattcatag ttgagcactt tctattatag aagtgcctct tctgatttat
      481 atcataaaat attactcaac aatttgtaaa atatattagt ttataaaata tattgaaaag
      541 ataatattat ataatacgat tgtgtaagat attgataaaa aatcgtaata aattttcatt
      601 agtatatgct aatatttttt tacaattttc gtaattaatt aatagaaaaa tatattataa
      661 taatattatg aaaatttaaa aaagtaagaa agaggtaata ttatgagaag tgatataaaa
      721 aaaggaatag aaggagctcc aaagagagcg ttaatgtatg gaatgggtct tactaaagaa
      781 gaaattgaaa gaccattaat aggtatagta aatgcacaaa atgaggtgat tcctggtcat
      841 ctgcatcttg atgaaatagc agaagctgca aaaaatggag taagaatgag tggtggtttg
      901 ccattagaat ttcctgctat aggggtatgt gatggtatag ctatggggca tgtcggaatg
      961 aattattccc tggcttcaag agaattaata gcagattcaa tagaagctat ggctatggca
     1021 catggttttg atgctttggt acttatacct aattgtgaca agatagtgcc aggtatgtta
     1081 atggctgctg caagacttaa tataccaagt attgttgtga gtggagggcc tatgcttcca
     1141 ggtaaaaaaa atggaaaagt atatgatttt aactctgcaa tggagggtgt tggagcatgt
     1201 aaagatggaa cagtaagtga agaagaatta gaagcgttag ccatgaactc ttgtcctgga
     1261 tgtggttcat gttctggtct tttcacagca aatagcatga attgtttaac agaagcttta
     1321 ggcatgggga taccatataa tggtactgct gcgtcacatt ctggagaaag aaaaaggatg
     1381 gcaaaatatg caggtatgta tgttatggag ttacttaaga acgacataaa acctagagat
     1441 attttaacaa tagatgcttt taaaaatgct atagctgtgg atatggcaat ggctggttct
     1501 acaaatacag tacttcactt acctgcaata gcttatgaat caggaataga gcttaactta
     1561 gatttttttg atgaaataag tgaaaaaact ccttgtttaa caaaattaag tccaagtgga
     1621 aaacatcata ttgaagattt acatatggca ggaggaatac cagctataat gaatgaactt
     1681 tcaaagataa atggaataaa tttagattgc aaaaccgtaa caggcaagac tataggggaa
     1741 aatataggaa attgtgaaat agaaaatgaa gaagtaatac atacattaaa gaatccatat
     1801 agtaaccaag gtgggcttgc aatattgaaa ggaaatcttg ctctaaatgg agctgttgta
     1861 aaaaagtcag cagttgcaga agaaatgtta gttcatgaag gaccagcaag agtttttaat
     1921 tcagaagaag aagctgtaaa tgctattttt ggtaaaaaaa taaacaaagg tgatgttata
     1981 gttataagat atgaaggtcc aaagggtggt ccaggaatga aagaaatgct atctcctaca
     2041 tcagcagttg caggaatggg acttgataag catgtagcac ttcttactga tggtcgtttt
     2101 tcaggggcaa ctagaggagc atctataggc catatttctc cagaagctat ggaaggtggt
     2161 ttaatcgggt tagttgaaga aggagatata atttctataa acataccaga caaaaaatta
     2221 gagttaaaag tagatgaagt tgaaatagaa aatagaaaat taaaatttaa acctttagaa
     2281 ccgaaaataa agcatggata cttaagtaga tatgctaaat tggtaacatc agcaaataca
     2341 ggagcagttt taaaatagta gactatattt atgtaaatta gaaaaagtta agaaaatatt
     2401 aattggatat cttaggtgtt atagcactaa tgaggtatcc tttttatttt gtatgaaaaa
     2461 cacagcaaaa tcttgattat ctttgaaaac aatacatctc tattataagg aatacgagta
     2521 cagcatagag atgtatcttt tagaccttgt ttaggtaata caacttttta aaagtttgat
     2581 gtggaatgct tttcgttcca tctgaactat gaagaaattg aaagtcagtt aattcttgac
     2641 ataataaata ttaaaatgta taataaaaaa caaacagaca gtctgttttt aggagggaaa
     2701 tatgaataga gaagaaaaaa gtaaaaatag taaagaaaaa attatccaat cagcattttc
     2761 actattttcg tctaaaggat atgattcaac atctacacaa gatattatca atttatctgg
     2821 tctatctaga ggtgcaatgt atcatcactt taaaactaaa gaagatatac tgagaagtgt
     2881 cacaaaagaa ctttactcac aaatgaataa ttttttagag catcttgttg ctgatgacac
     2941 ccttacagca aatgaaaaaa taatagaatt ggttgttcat agtgcgaatg attacacacg
     3001 tagaaaaatg gtacattgta gctggttaga aaaaatccca ttctctttaa tagaggaagt
     3061 tcgtaatctc aacaatgtag ttgcacccaa tattgctaag ataattaaac aaggtgttga
     3121 aaataaagaa ttttcttgtg aatatccaga ggaattagct gaaatgctcg tttttagtat
     3181 tgatatttta cttgaccctg tattatttaa acgtgaatac agtgaagtat gtaataggtt
     3241 ggatttttta ttattcatgt taaaaaaaat ggatattcct ctaattgatg agtacggtat
     3301 tcaaaaattt aaagatttat ttaaacaatg ataataggaa aagtgaggaa tagactatgt
     3361 tttataatgc aaaaagctgt caaatgatga taggaagtaa tacagtgaat tatattgaat
     3421 ttggtagtgg taagaagaca ctaattattt taccaggatt aggagatggt ttatttcctt
     3481 tacatggaaa aatacaagcg attgcttttg cttttagata taagcaattt gcaaaagatt
     3541 ataaggttta tgtatttagt agaaaaaatc aaattacaga aaaatattcc acaagggata
     3601 tggctaaaga ccaagcagat ataatgaaaa aacttggaat tatgaaagca gaagtaatgg
     3661 gtgtttctca aggtggaatg atagctcaat atctggcaat tgattatcct gagttagttg
     3721 agaaacttgt gttggcagtt acttcttcaa agcaaaatga tactatccaa aatgttattt
     3781 gtagttggat tgatatggcg aagaagcaga attacaatga tttaatgatt gacacagcaa
     3841 agaaatccta ttctgaaaga tatttaaaaa aataccaatt atttattcct tttttaggaa
     3901 aagtaggtaa accaaaggat tttaaacgat ttattattca agcgacctct tgcatagaac
     3961 ataatgcctt ttccgaatta aataaaataa catgtccaac cttaattata ggaggtgcta
     4021 atgataaaat tgttggaaat aatgcttctt ttcatttagc agaaaaaatt aaaaaaagcg
     4081 aaatatttat ctatgaggga ttgggacacg ctacatatga agaagcacag gattttaatg
     4141 aaagagtttt agagttttta aataagtaaa cacctttgtt aaaaatatac atatgaagat
     4201 tggaaattta atgttaaaaa tagaaacatg aaaatatgct taactggtat ttttactatt
     4261 cataaccaat ttttgataca ttatctacta taaatacaaa tatagcttca atgtgattat
     4321 atattggtgt attggtaaag cacttataca aacagaggaa ttttgtaaat tcatattata
     4381 cccacatttg ttaacttatg aaaatataat caaaattttt atgagatttt tatataaaaa
     4441 acgccctaaa aatcttatta tccccataaa cactgtatct aaaagcatat tcaataggaa
     4501 ataaatcgtg atattactac gaatttacta ctaatgaatg agccttgata cgtcttattt
     4561 cccagatatg caaagatatg gcatggcaca tcagtaaaaa ttgaatactt atatagacta
     4621 tggaacgtac acttttggcg tttcttttct atttccagat gttcttttca gagcgtcttt
     4681 ttttcatacc caaaatcgaa aggagaaaga gaaaatatga ataagctagt aaagcgattg
     4741 ctgacaggga cgctcgccat tgcaaccatt cttaccgcat tgcctgtgac ggtggttcat
     4801 gcttctggca atcaatactg gacagaatca gcagaacgtg tcggctacat tgaacatgtt
     4861 atgaatgatg gttctatcaa atccacatta aatgagggac acatgaaagt tgagggcgaa
     4921 actgcctatt gcgtggacat caatacaaat ttcaaaaatg gatataaaac aaggtatgac
     4981 gcaagctccc atatgagtag cgatcagatt gcggacattg ctctttcctt agagtacatc
     5041 aagcaatata ctgcttctca tacaaacttg aattacaagc agggttactt attggaacag
     5101 tgtgttgtct ggcagagatt gagtgaacag ctcggctggc aatgtgataa cgtcagagct
     5161 tcctataatg aaatctcaca ggcggtacag aataaagttt acgctggtgc gaaagtattt
     5221 gtgaaagcaa ataagggatg ctatgaatgt ggcggttaca tctacactgg cgaaggacag
     5281 gacattggac agttctgggc gaagttgaat gtgggaaatg aaaaggtcaa aaagacttct
     5341 tccaattcca ccctctacag atggcaatgt caattattcc tttgaggggg caacatttgg
     5401 cgaatcccat attgtagtca cagataaaaa cggtcagttc tctactgctt caagttgggc
     5461 tttccgtaag gtaaatacaa atgctggaaa atccagtgag gatggtgtat ggtttggtac
     5521 ttctgaacca gacgacagta aaggtgcatt acctatgtga ttgaggaatt aaagtgtgat
     5581 tccaacgccg gatttaagct gattccagct tttgaggtgg tcgtatccag aaaaaaagtg
     5641 accgtagatt tagggacact tactgatgaa tatgaaaaag aaatcacaat ccataccaca
     5701 gctaccgaca agaaaacagg cgaaaaaatg attgttgccg gaaaagacat caagatcgtg
     5761 gacaaagtca cacttgatgg cttggaaact ggcagtcaca gaaaattaca ccgtgaccgt
     5821 tcatgtgtat acagatggtg aaatgggtca ttacccagaa tcctaccctt gctccggtgg
     5881 tacagaaatc aaagtatgaa ccgaaagcac agggagcaga tgtcagcgtc agctccgata
     5941 cgttcaaaga cgctactact ttcttggaaa cattctttaa actctatccg acagctacag
     6001 aaaaagaact tgcctattat gtcaaagacg gtgtgcttgc tcctgtttct ggcgactatg
     6061 tattttcgga actggtaaat cctgtcttta ccaaagatgg cgataatctc aaggtcagtg
     6121 tgtccgtgaa atatctggat aacaagtcga aaatgacaca aatctcacag tatgagcttg
     6181 tgcttcacaa ggacgataat tggaaaattg taggataaat attacagcag accagctatg
     6241 acaatttcaa atacatatac taagttttaa aaatatacat gtaccctatt gactttttgg
     6301 gaaggtacct gtatattatt tgttaaaggt acctgttgaa aaggaggtca tactatgaat
     6361 aatatttatt ctgatatcta tgagaaacta tcaaccttgc aatggattat gaaacgccat
     6421 cagatgtttt gtcaggcaga gtctggtcca tttgctgata catcacgagg acaaggaaga
     6481 attcttgcta tgttaaaaat tcagccaaaa attgcaacaa aagagttggc atatttattg
     6541 ggaatacgcc aacaatccct aaatgagttg cttaataaaa tggagaaaaa tggctatgta
     6601 gaacgaaaac catctgaaaa tgataaacgc gttatgattg ttcatttgac agaaaaggga
     6661 aagcaagttc agcaaccgaa aacagactat caaaatatct ttaattgtct acttccagaa
     6721 gaattgctac aaatgtcgca atatttagat cgtattattg aagcttttca attgcaaaat
     6781 ggaaatgctt tagaagaaaa caatatgatt gactggatgg ctcaggcaag agaacgtatg
     6841 ggcgatgagc attttgaaca gttaatgtct atgcgtgaaa gagcttttgg acatatgaga
     6901 ccacccaaag atataccggg agctgaacgc ttttctgaaa actataacgg atatgttcca
     6961 gatagagacg gatttcagcc aagaaacttt aaaccagata tcaaataaca aaaggcactg
     7021 taaaattaca gcaataagcc ctatggagat tttttgaact ccatagggct tattttttga
     7081 aaaggagacg aacaaaatga accagaaaaa tgaaaattac tggcttttag acgcacctgt
     7141 cacaaaagct atatggcata tggcgattcc aatgatgctt ggaatgtcaa taaacattat
     7201 ttacaatatt acagatacat tcttcatagg cagattaaat gacacagcgg ctcttgccac
     7261 aatctcactg ctgttacctt tcacgaccat tttaatggca attgggaatt tgtttggaac
     7321 aggtggaagc actttgtttt cacgactgtt aggaagtgaa aatacggaca ggacaaaaca
     7381 atgttcagct acaacattat ggctatcttt cctgtttgga ctattgactg caatcatatc
     7441 catcattttc agtaactata ttatccgact tctcggtgct gatagtaata cttttgccta
     7501 tgtcaaacag tatcttattt tttatggaat gggtgctccg tttattattg ccaattttac
     7561 gctagaacag ttaattagag gtgacggtaa atctgtagaa tctatgattg gaatgatgat
     7621 aagcattggt gctaatatca ttcttgaccc aattctgatg tttggattac agcttggtat
     7681 tcgtggggca gccattgcta cagtaatcgg aaatgctttc gctgttatct attatattgt
     7741 ctgtatacaa cgagcagaca atcagttatc tgctcttcca aaatatttca ggcttgaaaa
     7801 acaaatgcta aaagaaattt ttttagttgg attatctgca atgttgttag atattctttt
     7861 gattgtttca agccttatgt ttaattacta tgcactaaaa tatggagatt atgtgcttgc
     7921 cggatttggg atttctcaaa aacttgtgca gattgtcgac ctaatcggca tgggacttta
     7981 catgggagta attccactta ttgctactgc ctatggtgca agaaatgaac ttcgtgtgaa
     8041 agaaatcatt aaaaagactg ctctctattt agcactagta attacatgtt tgtttactat
     8101 tctatttaca tgcagaaact ttattgttca ttgtttttca aatgattcag atgtaattcg
     8161 tataggtgcg tacatcttaa ccgttcaact ctgttcttct ttctttgcgg caggagcggg
     8221 tcttctaacg ggaatttttc aggcaaaagg agaaggaact ccggctgttg ttatgtccgt
     8281 tatgagaggt attatgctca taccagcaat tatcttcggc aattatctct ttaaaatgaa
     8341 tggagttatt ttttctctgc ttgtagccga agctatttca tgcattacag ggcttgtatt
     8401 atataaattg aaaaagtaat gctgaactcc atcagacttg ttaacaaagt tagtgatggt
     8461 tataaaaatc atcaacaata aattaagaga tttctataga atgagtatag attggtagga
     8521 gctggaaaaa tatgattggg cttaaaaaaa gaaatataaa aaaatgttga aaactggtga
     8581 tagagctgga gatgtatcat tagctgatat tcgggctaat atagaagcca ctattgatga
     8641 agacatgaac agtccagccc cagaggtaca ggcaaatttc aaaaagtatt ttggcaataa
     8701 acgtcctaca ccagaagaat atatttacaa gattacaaaa aaacaaaagt ttgatttacg
     8761 actatttgtt gcctgcggag tatatcaaca aataagcgtt aaagaatctg cacagaaaaa
     8821 cagagaaaat aagaagcgaa tcacttctta ttctctctgc tgttcaagat accatcaatc
     8881 gtaccttgaa tgatttttaa ttcgtcatca ctcaataaat caagtgacga atctatctga
     8941 cggctaactg tgcttttttc cacattcttc gctggataga aatactcgtc aacagatata
     9001 ttgaacatgg taactaaatc atggaataaa tgaaagctag ggtgtttccc gatattttca
     9061 atatctgcga tatgacgttc tccgtaaaag actttatcgc ctaaatcatt tctggaaagc
     9121 cctgctttct ctctggcttc tctgattgca agtcctaaag gacgaaaatc aaagctgtgc
     9181 gtatctttct tttttctcat ttttaatcac ctagcatgag tttatactat tttcacaggt
     9241 ttgctctgct caaggagcaa tttgtgattc taagtaaaac aggcagccgg caccgacaac
     9301 cgaatttatt cctttttttt agtttatcat cagcccttga gcatgaatta aaaactcata
     9361 tatcagatgt tttaaataca gtctatttgt tattagtgca gataggctgt tttcatattt
     9421 tcaaaaagtt tttcaattct gcaacaaatc acaccctgct gtacagatgt agaacggatt
     9481 ttgcggacat tcaaaataaa aaagaatgtg gaggtaacag acaatggcaa agacgatttt
     9541 tgaggaactg ggcggcaaat atgagcggca aggagattat ttaataccgt gcttgactgt
     9601 acccgccgaa gaagaacagc cgataggcac atggggacag cggcatctgg actatctgaa
     9661 gcagtaccga aaggttacat acaccaatct tctcacaagc ggcaggctca acacttacct
     9721 tgccgacatc gacaggcagg cgcaggaacg ctttgaacgg ctcatagagg gcatgaaaca
     9781 ggcgcagggc ataacagaac gcctaaagga agaaaatgcc ctagaatgga caggaagaat
     9841 gaataacata agggcgtgtg cgagggagat tgtgaacgaa gaaatcattt ttgcataagt
     9901 agagaagggc ggcagagtgt aaaagctctg ctgtcactgt tttaaattaa caatctgttt
     9961 tcgttgcata tatgacattt tcaaataaat cgacagagag ctgccctatg ctattgatac
    10021 aattcattgg aacccctata ctatatggtg tatagctatt tcccacaaaa gtgagaaatc
    10081 tgctgatgtg aaaaaaatct gaaaacaaac tgaatgatac aatgtgaaag aggaattttg
    10141 tatgaagaat gtatataaac ttgaatttca ttcgggaagc aaagaagaat tattgcctga
    10201 ttttgaatct gaatttccgt atattgcatc taatgcagaa cttgacaaat atattgggcg
    10261 ttctgcgccg tggcattggc atagggcaat agagcttttt tatatggaga gtggcacatt
    10321 agaatattac acgccgaaaa agaaattgtc tttttcggca ggttctggag gttttatcaa
    10381 ttcaaatgtc cttcacatga ctaaggctgt ttctaggaca gaaaaaaata tacagcggct
    10441 gcatattttt gacacagctc ttatttctgg aaactatggg agtaaaattg agaaaaagta
    10501 tgttatgcca attactgcaa atccccaaat tgaaatatat gctttccgcc ccgataattt
    10561 tatacacgca gatatattgg atttgataaa acagactttt accttatgcg agaatgacat
    10621 tggatatgag ataaagataa gggaatccct gtcccgaata tggctaaagc tttttgagct
    10681 gttttctgct tcatttgatg ggaaaggaaa atatgataaa aatgacgaaa gacttaagaa
    10741 gatgttaatt tacatacacg aacattttgc ggataaaatt gcggtttcag aacttgccac
    10801 agttgcatgt ttaagtgaaa gggaatgttt caggctgttt caaagctatt tgcatacaac
    10861 gcccacaaag tacatccaaa gttaccgttt acaagaagct tgtgaaatgc tcataaatgg
    10921 gcaggacaca attacagaca ttggacatgc ctgcgggtta gggaacagca gctattttag
    10981 caagattttc cgtgaatacc aaaactgcac tcccatagaa tatcggaaga aatggcagga
    11041 tagtcatatc ttatgtccga aataaaatag ataaataaat aattctgcgt tataatgata
    11101 tatagcacat attttgttat atcattttaa ggagaacgct atgttttacg aagccaaaga
    11161 ttttttggaa accgcagata aggaaacttt gcatattatt tccaatgcaa ggaaacttac
    11221 aagggaatac tattttacgg attatgataa tacaaaaaga aaaaatgaga ttttgaaaga
    11281 attgttggga gctgttggga aaaatgttgc cattgatgtc ccgcttcatt gtgactatgg
    11341 aaaaaatgta tttcttggtg atgatgtcgt tataaatatg aattgtacat ttgtggacaa
    11401 taagcccata cggattggca gtcgggtatt gatagcatct aatgttcagt tttatacatc
    11461 ttcccatcct gttttgccac aggagcgttt cgtttcggac tgggaagaaa ctgggacaac
    11521 atttttcaga acttatgccc gttccattga aatacaagac aatgtatgga ttggaggggg
    11581 aagcattatt ttagctggcg taacaatcgg tgaaaacagt gtggttggag caggaagtgt
    11641 tgttacccgt tcaattcccg caaattgtgt tgctgttggg aatccgtgta aagttattcg
    11701 ctgttttaca gagaaaagag gaaaagcata atgaaatatc aacatattgt ttttgacatt
    11761 gatgggactc ttatcaacac agagcatgca attttgcact ctttacaaga cacattagaa
    11821 gaatgtatac atgaaaaagt acctgtcaag caattgttat ttgtaatggg aattacggga
    11881 gaagatgcat taaggcagtt aaaaatcaat aatattccgt tgtctttgaa actatgggaa
    11941 gaaaacataa aaaaatacag taatacaata actgtttatg atggaatcca agaattatta
    12001 aacagtctat cagaatgcga ttgtaaaatt gggattgtca cctcaaaaac aaaagcagag
    12061 tttaaagatg attttgatag gtttgggctg aaacattatt ttaagacgat tatttgcgct
    12121 gatgatacaa cagggcataa accatccact gaacctttgt taaagtatat agaaatttct
    12181 ggggcataca acgagcaaat attatacata ggtgatagca aatatgacag cttatgtgct
    12241 aaaaatgcag aagttgattt tgcattggca atgtggggaa gccataccag agagttggaa
    12301 gccgattact tcattgaaac tccaaacgat ttactaggcg ctataaaata agcaggaatg
    12361 ataagaaaat aattgggcaa agaggtggtt ttttgaatat agaaaagtgg attttatgcc
    12421 ccgtttgcgg tggcaagaca cgtaataaaa ttagagagga tactgtttta ataaattatc
    12481 ctctttattg tcccaaatgc agacaggaaa gtttgattgt agcaaaaaat atgcaaatca
    12541 gtattgtcat agagcctaat gctcatacat acaaagcggt taattattag aatgatatta
    12601 acttgcagac aaggaggaca catggataat aatgaaaaat ggctgcaatg ggcagtagaa
    12661 ttacaaagtc tggcacaggc aggaattttc tatggaaagg acgtttttga taaagaacgc
    12721 tatgaaagaa ttagagaaat tgcagcagaa atgatttctt ataaatcaga aatttcaatg
    12781 gataaggtca aaaatctatt ttgtaacgaa attgggtatc aaacacctaa aattgactgt
    12841 cgtgctgctg tttttcaaaa taacaaaatt ttgcttgtgc aagagaaaaa tggaacatgg
    12901 tcattgcctg gaggttgggt tgacgttaat gtttcagtga aagaaaatgt gatgaaagaa
    12961 gtaaaagaag aagctggttt agatgtaatg gctgatatgg ttattgccgt acaggacagg
    13021 gaaaagcata atttaccagt ttatgcctat aaggtgtgca aaatatttat attatgttca
    13081 gtcatagggg gcgagtttaa atccaatata gaaaccatat cgagtaacta ctttgggatg
    13141 aacgagttgc ctatccttgc aacagaaaaa aataatgagg aacagataaa gatgtgcttt
    13201 gaagcatatc agtctgaaaa ctggaaaaca ttttttgatt agtatttttt agaaagagcc
    13261 agacacttta gatgcagagc caatgaactt gtgaaataga atcacagttt attggctcta
    13321 tttcatttca taagatttta aggcaaacgc ccaccaaata aaaaaaacga ggttcggtgg
    13381 gcggtattgc tatgcccgaa aagacttaac agacactctc cagacctgtt ttagagtttg
    13441 gagggatttt tttatgttct tttttcgggt agttattcat ctgctcatgc aacgcagagt
    13501 ttacccatac atagcatatc ggggaatggc aggaagccag tcaaaaaagt tcctgcttat
    13561 gcaacgctac ttcacgcctt gaaaccagaa gtagaatctc cacctaacag aatatgtatc
    13621 tcctataacc gtagaagtca cagaactttt gcggtttttc ttttgtcgtt ttttatcgga
    13681 atgtgccgca gggctagttc ttgtgttggc tgtcgttcgc cgccttttcc atctggattt
    13741 taacaattca aaaattcaga tgggaggtac ttacggtgaa gtatgcacca agaaaagtat
    13801 atatcaagaa aaatggcggt tatatggagt tatcctatac ggacttctgc cgccgcaggc
    13861 aagccgacca gagttacatg gacaagctgt ttatccccgt gcagggctgt ctgcttgagg
    13921 tagtgcggga acagtacgca gatttttaca aggagaaaga gcggtggcgt tatctgaaaa
    13981 agttggacac aaaccacaag ctgctctcat tagagggctt tacggacagt gagggaaatg
    14041 tgcttgactt cgttattgat gaaacggtgg acgttgcgga aaccgttgtc catgcggtga
    14101 tggtggacag tctgaaagcc gccctgtctt tattgtcggg cagagagcag gcattgataa
    14161 acgccatctt ctttgaagaa ctttccgagc gggaagtcgg gttgcggtta ggcgtgacac
    14221 agagcgttgt gaacaagcgc aaatccaaaa tccttgcgaa gctgaggaag ataatcgaaa
    14281 acaaaattta aggcgttcag cccccttgtt ttttcctttg ggaaaatgag ggggcttttc
    14341 cctgccccct caatcaattc tgattggagg aaaaaaagaa tggcatacaa ccacggacgg
    14401 gaggacagga aatggcgcat ctggaaagaa gcggaagaaa agattttgcg agagtgcggc
    14461 gttgatgaag aaaccattga gcaaatccgc acagacgaca gggcagattt taattccaac
    14521 aggcggtttt accgatgggc gagcgacttc ggcgaatacc ttgagggcat ggcagacagg
    14581 gagaagcagg cggaggtaaa gtctgtttct gatttactgg acgagattga ggacgaaacc
    14641 ctgtaccttg ctttagttac ggtagacagg cgcacgttac aaatcgtcct gctgaaaatg
    14701 cagggctatt ccactaagga gattgccccg cttgtgcatt taacggcagg ggctgtctat
    14761 gcgaggttaa accatctgcg gaagaagctg cggaaaattt tataagcgtc tcatatatcc
    14821 tgctttccta tgggctatcg ggtggggagg gaaaactctc tcctactttt tagcaggagg
    14881 aaagggaatg gcatacgggg caaagacata caagcttcgg gaggaatcca cggaaagcgg
    14941 cgcaaggtat tttatcagct ttaaggacgg gcagagggaa catcacgaac tggaagtgtc
    15001 tgaacaatta ttcattgaat ttcggcagat ggaaaggagg aacaggaacc ttcagcaatg
    15061 gaaccagcgg cacagggagt ttaacgaggt gtgggacgaa accctttaca gacgtgcgtt
    15121 aagagtgcct aaaacgcttg atgaaagaat gattgaaaaa gaacgaaatg aaatgttcct
    15181 taaagcggtt gcccggctgc cagagataca aaggcggcgt ttcctgctct attacgagta
    15241 tgatttcaat ttttaccaaa tcggcgcaat ggagcattgc acagcttccg ctgtccagaa
    15301 atctgtttcg gttgcaaggg agaaagttaa ggcggaaatg aggaagtatc tccaatctta
    15361 acgattactg cccgaaaaaa aaattcattt ttatcggcgt ggggatggcg gcattacata
    15421 ctctcacttc tttgtgtggg agtaaatcta tttaaggagg tcacgcctat gtgcaacaaa
    15481 accaaagaca ctgcccgaaa ggaggaaagc catgcagaag cttcagacag tcaatgccga
    15541 cacgctcctc tatgagccgc ttgagaagcc gtcctttgtg gtggacggtc tgatacccac
    15601 gggcttaacc ctgttctgcg gctcacagaa aatcggcaag agctggctta tgctaaagct
    15661 ctgcctatgc gtgtcgcagg gaatcccctt gtgggatatg ccgacacggg agggcgacgt
    15721 gctttacctc tgccttgagg acacgttctg ccgcatacag gacagattgt tccgcttgac
    15781 ggacgaagca agcgggcggc tctactttgc ggtggcgagc gacaagctgt cagacggtct
    15841 tatcgtgcag ttggaggatt atctgaaaga ataccccgac agcaggctca ttgtgattga
    15901 caccttgcag aaagtccgca ccgcatccaa agacaatgcc tatgcaagcg actacgggga
    15961 catctccctt atcaaagact ttgccgacag gcactcatta gcggtcattg tcgtacacca
    16021 catccgaaag cagaacgaca gcgacgtgtt caacaaagtg tctggcacga cgggattgac
    16081 ggggagtgcg gacgcaacct ttgttctgga acaggaaagc cgtgcgtcca atgccgcaaa
    16141 gctgtatgtg acgggcaggg acacgcccta tcaggagttc acgctccgct tccgtgattg
    16201 cagttgggaa ctggtggaac ggaaagagca ggaacagctt gccaaagaag cgataccaga
    16261 tgttcttttt cggctggtgg attttatgca ggacaaggag gaatggacgg gaacagcaac
    16321 ggagctgtta gccgctatgg gggaaacgga aaccgtcccc acggtgatta cgaaatggct
    16381 caatgaatac cgcaccacgt ttttaagcga aaatcatatc tgctaccagt acagccgcaa
    16441 aaaagacggc aggcagatag ccctcgcaag gcagacgggt gacagcggtg acagtgatga
    16501 cagcggtatt gggatacccc ctgttactgt cattgacacc taaagccatg taaagctggc
    16561 ggctctgccc tgcgggtgac agcggtgacg gtgatgacgg caatattggg atacccccgc
    16621 cgctgtcatc cctacgggag aacaccccgt aaacgcaaga tgcaggcgtg ggcattactc
    16681 gcccttttcg gctcgtaaag ccacccctgc ggtcagaaaa atctaccgat tttcccgact
    16741 gcgtttacag ggtgtaacac actacacttt gccctgcaaa gagccgtgtg ccagacgttc
    16801 cctctggact cccttaaagc agggcttacg ccctgcccat cctgcgcctt gcggcttcgg
    16861 atggaagaat gtgcggtgac agtcggcggc taagaggggg tatcccaaaa acaccgtcat
    16921 catcgttata accgtcacgc accagagaaa gacggcagga cggtgacagt gacagccaag
    16981 aggggggtat cccaaaatca ctgtcatcac cgtcaccact gtcacccaaa ggacagttac
    17041 ccgccgaaga aaggaggaat cctgccatgc cctatgcaat cctgcgtttc cagaaacgca
    17101 aggcaggcgg cgttgcggct tgcgaacgcc acaacgagcg gaagaaagaa gcctataaaa
    17161 gcaatccaga tattgatatg gaacgctcta aaaacaatta ccatctcata gcaccgccaa
    17221 agtacaccta taaaaaagag ataaaccgaa tggtagctga agcaggatgc aggacgagga
    17281 aagacagcgt gatgatggtg gaaacgctca tcaccgcatc gccagaattt atgaaccagt
    17341 taccgcccga agaacaaaaa gagtatttcc agacggctct tgactttatt tcggagcgtg
    17401 tgggaaagaa aaacatcctc tccgcagtcg tccacatgga cgagaggacg ccccatatgc
    17461 acctatgctt tgtgccgctt acgccagaca acaagctgtc agcgaaatcc attttaggca
    17521 accaaaagag cctttccgag tggcagaccg cctaccatga gcggatgttc gcaaggtgga
    17581 gcgaattgga aagggggcaa tcctcaatgg aaacgaagcg gaaacacatc cccacatggc
    17641 tctataagtt gggcggcagg atggataaac agtatgaaga aatcgtgtct gccctctccg
    17701 acatcaacgc ctttaacgca ggaaagaaaa gggataaggc gttagaactg ctctccgcat
    17761 ggcttccaga cgtggagaaa ttctcaaagg aaatcagtaa acagagtgct tatattgaca
    17821 gtttaaaaga gagaatcggg caggaatccg actatgcggg gcgtatgcgt gatgagaagt
    17881 acgagcagga acgaaaggta cagaaagcca accagaggat atttgagttg cagaaaacca
    17941 atcagcagat ggaacggtta ttgaaaaaga taccgccaga agtgttagag gaattgcaga
    18001 aaagcaatcg gaatagagca agagaaaggt aggtagcaga ttgaagaaac aggattttaa
    18061 ggtattaaag accgcagatt tatacccgtt tcccgacaac ccgtttcatg tggtggagga
    18121 tgaaatgctg tcagagttgg cggaaagcat taaggagttt ggcattgtaa cgccgataat
    18181 cacacgcccg aaagaggacg ggaatggata tgaaatcatt gcgggacagc ggcgtgtccg
    18241 tgcttccgag cttgcaggga taaacacaat ccccgctttt gttctgccct tagaccgtga
    18301 ccgagccatc atcacacttg tagacagcaa tctccaaaga gaaaatatcc tgccatcgga
    18361 gcgggcattt gcctacaaga tgaaatccga agccatgaag cggcagggtt tccgaacaga
    18421 tttaacctcg tcacaagttg ggacgaagtt gcggacggac gataaagtgg cacagggctt
    18481 cggcgttggc aggatgaccg tgcagagatt tatccgatta acggaactga taccgcctat
    18541 tttgcagatg gtggacgagg ggaaaatcgc ccttacgcct gcggtggagc tgtccttttt
    18601 gaagaaaggc gagcaggaaa acctattcgc cacaatggag agcgaggaag cgacgccctc
    18661 actctcacag gcacagcgga tgaaaagcct aagccagagc aggcggctta acatggacgc
    18721 tatctttgcg attatgacgg aggaaaaggg aaaccagaaa gaaaccgtga aaatcggcat
    18781 ggagcggtta aagaaatact tcccgaaagg aacaacgccg aagcagatgg aggacaccat
    18841 catcaaactt ttggagcgtg aattgcagag gaaacggggc agggacagcc gctaatcttc
    18901 tcttttcgga gggtaaatac aaggaactgg acgtaagaaa ggtcaaggca ataagaccag
    18961 aaaggggcag gaaatgaaag aaatccagta tgagattgta aaggaaattg ccgtgttgtc
    19021 tacgagcgaa agcggctaca caaaggaaat caatttaatc tcatggaacg ggaaagagcc
    19081 gaaatatgac atccgcagct tttcccccaa ccgtgagaag tgcggaaagg gaatcacgtt
    19141 gaccgctgat gaagcggcgg ctctccttaa agcattgcag gaagaattga acagcgggga
    19201 ttgattgtgt ctgattggca gggcgggagc atttttatat actcccctgc cctgtctgga
    19261 aaggaagaat tgagtatggg cgaggataag aaagcgaaca aaaagagaaa gcgcattgtg
    19321 gcaagacagc cagtgcagat gattgtcagc cgtgaatatg tcggcacaca gaccgttgcc
    19381 gaagcgttca tcccgattat ctccgaggac atccgcagga agattgccga aggtgacacc
    19441 ttcgacaatg aggatatatc cgcttagaat gtacgcaatg gaacatgaaa acagttaggg
    19501 cagatacgga ggttttacag tatggcagga atcaaagaag aaaagaaaat ctattcagtc
    19561 ggcatttact gccgcttatc taaggatgat ggcacggaaa acgagagtgc gagcattgcc
    19621 acacagaaat ccatcctcac ggattatgtg aaaaagcagg gctggcatct ggcaaaaacg
    19681 tatgtggacg acggttactc tggtacaaat ttccaaagac cgagtttcca gaacatgatt
    19741 aaggacattg aaaacgggct gataaactgc gtgattacaa aggatttatc ccgtctgggg
    19801 aggaattatc ttgactgcgg actatatctg gaagtctttt tcccagaaaa taatgtgcgg
    19861 tatatagcgg tcaatgatgg cgttgacacc ttaaataaat cggcgatgga catcacgccg
    19921 ttccgcaaca tcctaaacga aatgtattca gccgatgtgt cggtcaagat aaaatcggca
    19981 tatcgagcga ggtttcagca gggaaaattc atggggacat atgcacccta tggctatatc
    20041 aaagaccccg ccgaccacaa ccatttgctg atagatgata aggtggcgca cgttgtaagg
    20101 gagatattcg accttgcatt agcgggaaat ggacttgcaa aaatccgcaa gcacatcaac
    20161 agtcagcata ttttacgccc tgccgcttat gcggcggagc agggggcggc aggctatgaa
    20221 cggtactttg agggtaatga ggagaaccgc tatatttgga gcgagaacag cgtgaggggc
    20281 attttgagaa gcccaatata tgcgggaaac cttgcaggct acaagcggat tgccgccaac
    20341 atgaaaagca agaaacgccc ctctaagctg cccgaagaat gggaagtgat acccgacacc
    20401 catgagttct ctgcgaaccg tggtagctag aagttcgatt atcctcttgg gagcgtttaa
    20461 tgccgtaaat taaagcataa gtgcttgtta tgtttaattg ttgatactct ctggtgtacc
    20521 ctcatacacc gtagagtgtt agcaggttgt tagcaacaaa ggctaattta aagctttaat
    20581 ttatcagaaa acattacaaa caaactataa atcatttatc actagaaatc aagatactcg
    20641 tatgttatag acgttcagaa atgagcgtct atttttatac caaaaatgaa aggagtatta
    20701 aaatttatga aaaagacatt gaaacgattg tgtacgggct tcttagctct tgcaactatc
    20761 gttactgctt taccgactac acccgttcat gcagaaagta agcaatactg gacagaaagt
    20821 gcagagcgtg tcggtatcat tgaaaaagta atgaatgacg gttctatcgg ctcgacattc
    20881 aatgagggca tgatgaaagt tgagggcaaa acagcctatt gtatcgacat taacacagat
    20941 tttaggaatg gctacaagac cagagctgac gcaagctcac gcatgaatgc caaccagatt
    21001 tcagatgtgg cgttatcctt agagtatgtc aaacagtatg gcgaagctca caaggaactg
    21061 aactacaagc aagtctatct gttggaacaa tgtgtagtct ggcagagatt gagcgtacat
    21121 cttggctggc aatgcgataa cgtgcgagct tcttatgatg aaattccaaa ggcaacgcag
    21181 gacgaagttt tctctggtgc aagaaccttt gtcaaagaaa ataaaggacg ctatgaatgt
    21241 ggcggttata tctactctgg cgaggggcag gaattggggc aattctgggc gaagctgaat
    21301 gttggaaatg cgaaacttaa aaagacttcc agtaatgcca gcattacaga cggtaacggg
    21361 aattactcaa tcgctggtgc gacctatggt gtctttactg ataaggactg cacgaaacag
    21421 cttgccaccc ttacgactga tgaaaacgga aatacagatg ttgcagaggt aaaagcaggc
    21481 acagtctata tcaaggaatt atccgcacca gcaggatata aagtagataa aactgtatat
    21541 tccttaaaga ttgaagctgg aaagacggcg actttgaacg tatctgatac gccaaaagta
    21601 acggacactt tgattgagct tttcaagata gatatggaaa caaagaaaga caatccgcaa
    21661 gggaacgctt ctttagcagg tgcggaattt acatggaagt attatgctgg cttctataac
    21721 aaagacaatc tccctgccga agctactcga acatgggtta caaagacaat cgctgaaaca
    21781 gacagctacg ggacaactca ttacatcaca aaattagcgg acgcatacaa ggtgtctggc
    21841 gacagcttct atatgcaaga cggaaaagcc gttcttccac ttgggacact aaccatagaa
    21901 gaaacaaaag ctccaaacgg ctacttgtta gagggtgcat atatgcaggc tggcgataag
    21961 tccgaacaga taaagggctt atatgtaaca cagattaccg aggacgacga ccttgccgta
    22021 ttatctggaa gtaaccagtt ttccgtatca gacaaggtta tccgtggcgg tgtgaaaatt
    22081 cagaagcgag atttagaaac gggcgatatg aagccacaag gaagtgcaac cttgaaagat
    22141 actgcttttg acatcatttc cttaaatgat aattctgttt tggttgaggg caagctatac
    22201 aagaaaaatg aagtggtaaa gacaattcgt accgatattg agggtgtcgc ttctacttct
    22261 gctgacctct taccttatgg aaaattccgt attgttgaga gtgaagctcc cgacggttac
    22321 ttaactgacg gtgcaaagcc gattgatttt acaatcacag aaaatggaaa aatcgtggac
    22381 ttaaccgaca aagcccattc tatctataat cagattaagc gtggagatat tgagggtgta
    22441 aaaatcggtg caggcacaca caagcgtctt gctgatgttc cctttaggat cacaagcaag
    22501 acgacaggag aaagtcatat cgtggtaact gacaataacg ggcagttctc cacttctgct
    22561 gactgggctt ctcataagca caataccaac gcaggcaaga ccagcgagga cggtgtgtgg
    22621 tttggaactt ctgaaccaga cgacagcaaa ggtgcgttac cttacgatac ttacgtcatt
    22681 gaggaattac gctctgaaag caacaaaggc tttgaactta tcccaccttt tgaaatcgtg
    22741 gtatcaagaa ataatctttt gattgattta ggaacgctga ctgatgaata cgaaaaagaa
    22801 atctctatcc ataccacagc gaccagcaag gacggcgaaa agactatcct tgcaggaaaa
    22861 gagattacga ttgttgatac tgtcaaatta gacggactta caaaaggcac aaaatatcag
    22921 ctcaaaggct ggcagatgtt aaaagaggaa aacgccgagc ttatcattga cgggaaacgt
    22981 gcagaaaatg attatacctt tgtcgctgat gaggaagaaa tgaaagtgga aattgcctat
    23041 acattcaatg cgtctgcttt aggtggcaag aaccttgtta cctttgaaga attgtatgat
    23101 ttgagcaatc cagacgaacc cgtgaaagtt gcggaacaca aagacattga ggacgacggg
    23161 cagaccgttt taatcacaga gcgtatcatc aaaattcata cgactgctac gaataaggac
    23221 ggcaacaaag agcttgaagc tggaaaaaac gttacaatca ttgataccgt aaccttagag
    23281 ggcttagaag ttggtacaca gtacaaactt gtgggctggc agatgttgaa agaagaaaat
    23341 gcagaacttc ttatcaatgg aaaatgtgtg gaaaatgatt atacgtttac tgctgacagc
    23401 gaaactatga aagtggaagt tgcttttacg tttgacgcta cttctcttga cggtaaacag
    23461 cttgtaactt ttgaagaatt gtacgattta agcaatccag acgagccgaa gaaagttacc
    23521 gagcataagg atattgagga taagggacag acgattacct ttaaggaaaa gccagaagaa
    23581 ccagagaaac ccgaaacacc acagatacca gaaaagccga gcagaccgag cgacagtccc
    23641 aaaacgggcg acagtacaaa tgtaatggca tttcttgtga tgttgcttgc gtctgctggt
    23701 ggactggctg gaacatatct ttacaaacgc cgtaaaatga agaaatcata aggcggtaac
    23761 ggtatgatga aagaaaaatc acgctctccg ccgaagctgt caaacggcag acttttgctt
    23821 attctggctt gtctgccacc aacctgcaag aacacggata gtcaagggtg cgaagcattg
    23881 attttaccct ttactatctg ggggattgct ggtacttccc aaaccgccag aataagaaat
    23941 tacaatcaat ttatcaaaat agaaagaaac gaggtaaaac attatggaaa tgaaatatgt
    24001 cgtgccagat atggcacagt cttttggaac tcttgaattt gcaggagaaa gcgagcctat
    24061 ctttgaaaga gataaaaata accgcagggt cttagccaga cgaagctata acctttattc
    24121 tgacgtacag aaaggcgaaa atgttgtagt ggaaattccc gtgcaggctg gcgaaaagaa
    24181 gttcaagtat gaacagaaag taaaacttgt caatccgaag ttgtacggca gaggttacgc
    24241 aatcggggat atgggacata ccgattatgt attagttgct gatgatattg tagcagttga
    24301 agaaaagtaa aggagtgaag aatttatgag attatcaaac gggtttgtta ttgacaaaga
    24361 gaaaacattt ggcgaattaa aatttacagc ggtgcgtgat gtgtttatgc agaatgagga
    24421 cgggacaccg agtacccagc ttaaaaagcg tatctatgac ttgaaatgta gtctgcatgg
    24481 tggaattatc cccgtgtccg taccgccaga agtgccgtta agggaatttc cgtacaatgc
    24541 agttgtagag cttgtcaatc ccgtagccga tacggtatca agaaagacgt ttggtggtgc
    24601 agatgtggac tggtatgtaa aggcagagga tattgtgttg aagaataaag gcaatcagaa
    24661 cgcaggcaat ccacagaacc atgtaccgca gggacagacg aaaaaataaa tctaggtcat
    24721 agattatact ctgaaattac agtataatga gccaaaagag ccgtatttaa gctatgtaaa
    24781 ataattttga caaggaaaaa aaggatttgt cgaagatatt ggatagacat ttgagggtta
    24841 atatatgaca atgacaccga aaggagcaat cggaatatgg aaataggtaa gaaacttaaa
    24901 aatgcacgag tacagtctgg aatgacacaa gaaaatgttg cagaaaaaat caatgtatca
    24961 aggcagacaa tttcaaactg ggaaaatgaa aaatcttacc cagacattat caatgtaata
    25021 gagttgagca acctatattc tataagtctt gacgaattgc taaagggtga tgaaaaaatg
    25081 attgaacatt tagaagaaag tacaaatgtt gtaaaaagca accaaaaaat gatatgggca
    25141 attattgtaa atatcattgt cgtagcattg ctgatagcat taaatatgtt tataccagat
    25201 aaccgttatt tcttagttgg aatattttgt cttatggtta tcacttcatc agcattactt
    25261 taccagataa tcaaaaaatt ttaaggagga tattttgtta tgaatttgaa catagtttta
    25321 gcggtaattt gtggagcagt tgcacttgta ggagcgtttt gtgttgtgtt tcagatttat
    25381 catatgactg ttatagacgc aacagccaga ggattaaagc accctaaatt ttggggagta
    25441 tttacaatga gtgggaataa ttcaagtggt ttgcttatgt atctaatcgg aagaagaaaa
    25501 taccctattg taaatatgag tgaaagcaac tcaaaagaat tagaaaaacg taaaaaatca
    25561 gcaggtattg gactgttatt tttggctatt ggtgtaattg gcattatatg tgcgacctta
    25621 atataactaa aaatgcagat ttatgattat atcttgcaac atttacaaag cagttagtct
    25681 atatgattga ctgctttttt cgtacccaga aaggagtgaa tgatttatgc gtaagatttg
    25741 gaatagagga caccgtatca gagccagcga caagcacctt gtctaccatt tttccatagg
    25801 gacgcttctg tttttattcg tggcggttct cctgctactg aatatcaaac agctcatgcg
    25861 taccgactgg gagcatttca gcttgttaga gaacggcttg acgctttccc cttacaactt
    25921 cataaccata ctgatagcga ctggtgtttg tgcattggtc gcttttctgt attaccgctt
    25981 ctgttacgac agtttcaaga agctcctgca ccgtcaaaag ctggcgagaa tgatactgga
    26041 aaataagtgg tatgaagccg ataccgtaca agacagcggt ttttttactg acctgcaaag
    26101 cagatcaagg gaaaaaatcg tctggtttcc aaagatttat tatcaaatgg aaaagggact
    26161 gcttcatatc cgctgtgaaa ttacgctggg aaaatatcaa gaccagcttt tacggttaga
    26221 ggataaattg gaaagtggat tatattgtga gctgaccgac aagaccttac atgacggtta
    26281 tatcgaatat accctgcttt atgatatgat agcgaaccgc attactattg atgaagtacg
    26341 ggcagaaaat ggctgtctta aattgatgaa aaatcttgtc tgggaatatg acgcactccc
    26401 tcacgctctg attgctggtg ggactggtgg cggtaaaacc tattttctgc tgacgctcat
    26461 tgaagcctta ctgcatacca acgctgtcct ttacatctta gacccgaaaa atgcggactt
    26521 agcagactta gggacagtta tggaaaatgt gtatcacacc aaagaaaaaa tgattgattg
    26581 cgtcaatgcc ttttatgagg gtatggtaca gcgaagtgag gaaatgaagc gacacccgaa
    26641 ctataagacg ggcgaaaact acgcttatct gggactgcca ccctgctttc ttatctttga
    26701 tgaatatgtg gcgttttttg aaatgctggg gacaaaagaa agcgtgggct tacttagcca
    26761 gttaaagaaa atcgttatgt taggacggca agcaggatat ttccttatcg ttgcctgcca
    26821 gcgtccagac gcaaagtatt tctcggacgg tatcagagat aacttcaatt tccgcgtggg
    26881 acttgggcgt atcagcgaat taggttacgg tatgctgttt ggttcagatg tgaaaaagca
    26941 gtttttccag aagcgtatca aggggcgtgg ctattgtgat gtgggaacaa gcgttatcag
    27001 cgaattttac acgcctttag tcccgaaagg acatgacttt ttgcagacta tcggctctct
    27061 tgcacaagca aggcaggacg ggacggcgac gtgcgaagcg aaaggcgacg gcacggacta
    27121 gccgttgctg gtgtggcgta agccacgcca gcaggtaaaa cccctcggta tctaacagag
    27181 gggtacgttt gcaaatagac aatagacgaa aaacatagga aacataaggc ttctggcatg
    27241 atttcccagt aaaaatcggc tgtgaagtcg ccttaaaaaa cttcacactt gacagattgg
    27301 gggtatggtt ctgaatgaag aacaatggat aaaagaatta cgggagaaac ggattgctta
    27361 cggtatctca caaggcaggc tggcggtggc ttctggtatc acaagggaat atctcaacaa
    27421 gatagaaagc ggaaaaatga agccgtcaaa ggaacttctg gaaactctgc ataaagaact
    27481 ggcaaggttc aatccagaag caccgcttac catgctgttt gattatgtga aaattcgttt
    27541 tcccacgctg gatatacagc acatcatcaa agatatatta aaactgaata tcaactatat
    27601 gctccatgaa gattacggac attacagtta tacggagcat tattctttag gggacatctt
    27661 tatctatacg tcggctgacg aagaaaaagg tgtcctttta gagttaaagg ggcgtggttg
    27721 ccgacagttt gagagttacc tgctggcaca gcaaagaagc tggtatgact ttctcatgga
    27781 cgcactcata gacggtggcg tgatgaagcg tatcgacctt gctatcaacg accatacggg
    27841 cattttggat attccagagc ttgcggaaaa atgcagaaaa cgggaatata tcggaaagtc
    27901 cagaagttat aagttttacc agtcgggcga gcttatcaag cacagcgagg acgacagaga
    27961 atatatggga cgtacccttt atcttggttc gctgaaatct gatgtatatt tctgtatcta
    28021 tgaaaaggac tatgagcagt atgtcaagtt agggacacct ctggaagaag ccgatattat
    28081 caaccgtttt gagatacggc ttagaaatga acgtgcctat tatgcagtac gggatttgct
    28141 gacgtattac gacgcagagc agaccgcctt ttctatcatc aatcagtatg tacggtttgt
    28201 tgatgaagaa ccagacaagc ggaaaaatga ctggaaactc aatgaccgct gggcttggtt
    28261 tatcggcgat aatcggcaaa gtctgaaact gacgacaaag ccagaacctt ataccttaga
    28321 tcgtacattg cgttgggtac aaaggcaggt agcaccgacc ttgaaaatgc tgaaaaagat
    28381 tgataaggga aacggtacag attacatgga gacaatcgaa cagcaggcaa agctcacaga
    28441 aaagcatgaa atgataatca aacagcagac gacccctgca aaagatttag tagaaattta
    28501 ggagtgatga aaaatgtggg tattattaaa agacttcctg ctggtatcta tgggaatggg
    28561 tatcggcata gtcttgatgt gtattttgaa tgtcggcaaa gaagctgact ataaaatgaa
    28621 acaattagaa gaaagcgagg acaattaaat gaatttcgga caaaatttgt atcaatggtt
    28681 tttaagtaac gcacagagct tagtgcttat ggcgattgtg gttatcggta tctacttagg
    28741 gttcaagcgt gagttttcca aacttatcgg cttcttggta gttgccttga ttgctgtcgg
    28801 tttggtattc aatgctggcg gtgtgaaaga tgtattgtta gagctgttca ataagattat
    28861 cggtgcataa aattttattg tggtactgat atgcgaatgt tataatttga atgttgtatt
    28921 acataaaacg ctgtacttac cagaaaaatc tatgaatttt gtatattata caaggttgca
    28981 acttgaagtt gctaatgtgc atttcaaaaa atatagaata taacattatg gcttgataaa
    29041 atttattcat caaatcatag gaggtatcac acaatgagtt taggagaaaa aatcttcaag
    29101 ttaagaaaag aaaaaggaat atcgcaagaa gcactagctg aacaaatagg cactactagg
    29161 caggcaatta gtaaatggga aaacaatcaa gggtttccag aaacagaaaa gcttttacaa
    29221 ttatcaaaca tttttgaagt atctacggat tatcttttga aagacgaaaa gaatgtaaag
    29281 aatagtgaag aacgaggata ttatgtaagc aaggaaatgt cggtagggtt tatcgctaat
    29341 cagaaaaaat taggacttta tcttgcttta ggggttatgc tttgcatttt tgctggaata
    29401 ccatatacct tgtttttaga taatatgact tggcgttatt taggtatggc attttgcttt
    29461 gtggcaggaa ttatattttt tgtcgtagca attttttatg aaaaacaaga atacaacatt
    29521 ttaaggcaag aacctttact acttgattat gaatatctaa aagaattgaa aagcgaatac
    29581 acttcaaaaa agaaaaaata tgtaatagtt gctgttcctt gtactgttct ctttatagca
    29641 ggactaattt tctttctatt tactgtgaaa ggctatattt catggacaga atatcattcc
    29701 tttgcatttt tcggtcttgc tgttggaatg ttgggttttg tatatacaat caatgttatg
    29761 gaaacctatg aactattagt gaaaaacgag caatattcaa cgagtttctt tttcaaacta
    29821 aaaagaaaga ttaaaaagag attaggataa gctataattt aatattcaca caaagcagtt
    29881 agtcttatga ttgactgctt ttttcatacc aaaatttcag attggagtga ttacatgaac
    29941 gatattttta aggatatgca ggcaaaagtc ggctgtgaat acatttctga cctgccctct
    30001 tacaagcgta aggtgtggcg tgaaatgaaa cggctgagcc ctgccgatta tgaagaaaga
    30061 caattagaag atttttcaaa gtttgtgttt ggtatgccgt accagacctt aaaagatgtg
    30121 atgaaacaac agaaaggacg tgaagaacaa tgcaggaaac aagggtgctg gtggaaacga
    30181 gagaaacaac tggcgaagaa acaacatcat actggttcga cctgccgata gatgttgccg
    30241 agtttgaaga aaagttaggt gtcggtgcag aaagcacggc gtaccgcatt atcgaaaagg
    30301 tgttgcctta tgctgatgaa gtccacgaac atacaagcgt gtaccagctt aacgaattag
    30361 agtttatgta ccgccagctt tcaagcgata tgcaggaaga atatgtatca ctacttacgg
    30421 tgtttgagaa tttagaagca ctttatattt gcaggaacgt gattacggtt tatcccgact
    30481 gcaaaagcat gatagatgtt gcaaggcaaa agctgatgaa tgactcgacg ttcaaacatt
    30541 tatccgagga ctgtcaagaa tactactttg actttgaagc ctacgcttct cacttgcagg
    30601 aacacgggaa attcttagta acggaacacg gtatctttga actaccagag taggaaatga
    30661 ggtggtgctt atgatagatg atatggcggt ttacattgct aatctcggta aatacaatga
    30721 gggttattta gtcggtgctt ggttcacgtt ccccattgac gaggaagatg taaaagaaaa
    30781 aatcggcttg aatgaacagt atgaggaata cgctatccat gataccgaca acttccccat
    30841 tgcgattggc gagtatgttt ccattgaaga actcaatgag atgtatgaaa tgatagagga
    30901 acttcccgac tatatcgtag agtgtctgga cgaatttatc agtcactacg ggacgctgga
    30961 agaagtcgtg gaacacaagg acgatattta ttattatccc gactgtgaaa ccatgacaga
    31021 tgttgcctat tactacatag acgaattgca ggcacttggg gacattccac ccagcttaca
    31081 gaactacatt gattatgaag cctacgggcg agatttggat atgggcggtt gctttattga
    31141 aacaagccga gggatatgcg agataccata ttaacgctgg aagatcagca acggcatggt
    31201 tgctactgac agcgtatttc tcttttaagg gacagtctga aaatggctgt ccttttctct
    31261 ttgaagaact tacgaaagga gctgaaagaa cttgaaaaag attaaatctt atacgggtat
    31321 ctggaacgtg gaaaaagtct tgtatgcaat caatgacttt aacctgcctt ttcccgttac
    31381 ctttacacag attacatggt ttgtgattac ggaatttatc atcattctgt ttggggatat
    31441 tcccccactt tccatgattg agggagcatt tctcaaatac ttcggtattc ccgttgctct
    31501 cacttggttt atgtcgcaga aaacctttga cggaaagaag ccgtacagct ttttgaaatc
    31561 acagataacc tatgccctgc gacctaaaat cacttatgcg ggaaaagccg taaaactgca
    31621 taagcagacc ttgaatgaaa caatcacggc agtaaggagt gtgaactatg ttcccgataa
    31681 aatatattga caataacctt gtctggaaca aggacaatga ggtgttcgct tactatgagc
    31741 tgataccgta caactattct tttctatccg cagagcagaa atttatcgtg catgacagtt
    31801 tccgacagct tatcgcacag tcccgtgagg gtaaaattca tgcgttgcag attgctacgg
    31861 aaagctcaat ccatagtatg caggagcagt caaagaagct ggtaactgga aaattaaagg
    31921 aagttgcctg ccagaaaata gacgaacaga ccgaagcgtt agtatctatg attggtgaca
    31981 atcaagtgga ctaccgcttt tttcttggct ttaagctcat ggttacggaa gaacagctca
    32041 atctgaagaa cataaaaaaa tcggcgtggc tgacgttcac ggaatttctc catgaagtga
    32101 accacacgct gatgaatgat tttgtttcca tgccgaatga tgaaatcaac cgttacatga
    32161 aaatggaaaa gttactggaa aataaaatct cccgtcgctt caaggtgcgt cgcttggaaa
    32221 tcaatgattt tgggtatctc atggaacatc tttacggcag gaacggtatc gcctatgaag
    32281 attatgagta tcagttacca aagaagaaat tgaacaaaga aacgctgata aaatactacg
    32341 accttatccg tccgacaagg tgtgtgattg aggaaagcca gcggtattta cgattggaac
    32401 atgaggacaa ggaaagctat gtgtcctact ttaccgtcaa tgcgattgtc ggggagcttg
    32461 attttccgtc aagtgaaatc ttctatttcc aacaacagca attcacattc cccgttgata
    32521 cttctatgaa tgtagaaatc gtggagaatc gaaaggctct taccacagta agaaataaga
    32581 aaaaggaatt gaaagatttg gataatcacg cctatcaagc aggaagtgaa accagttcaa
    32641 atgtggtgga cgcattagac agcgtggatg agctggaaac ggacttagac cagacaaaag
    32701 aaagtatgta taagctctct tatgtagtgc gtgtatcggc tgatgatttg gacgaattaa
    32761 aacgccgttg tgatgaagtc aaggactttt acgacgacct caatgtaaag ctggtgcgtc
    32821 ctgctgggga tatgctgggg cttcattctg aatttttacc tgccagcaag cgatatatca
    32881 atgattatgt gcagtatgta aaatcagatt ttttagctgg gcttggcttt ggtgcgacac
    32941 agcagttagg agaaactacg ggtatctata tgggctattc cgttgatacg ggaagaaatg
    33001 tgtacctgca accgtccctt gccagtcaag gtataaaagg tacagttacc aacgcccttg
    33061 cgtctgcttt tgtcggttcg cttggcggtg gaaagtcgtt ctgtaacaat cttctggtgt
    33121 attattcggt tctctttggg ggacaggcgg ttatcttaga cccaaaatca gagcgtggca
    33181 actggaagga aacacttcca gaaatcgcac atgaaatcaa tatcgtaaac ttaaccagcg
    33241 ataaaggaaa tgcaggactt cttgacccat tcgtgattat gaagaatgta aaagacgctg
    33301 aaagtctggc aatcgacatc ttaacattcc ttacgggtat ttcctctagg gacggcgaaa
    33361 aattccccgt acttcgtaaa gcggtacgct ccgttaccca gagcaacagt cggggcttgc
    33421 tccatgtgat agacgagcta cgccgtgaag atacgcccat atcaagaaat atcgcagacc
    33481 atatcgacag cttcacggac tacgactttg cacatctgct gttttcagac gggacggtag
    33541 aaaatgctat cagtctggat aaccagctca acatcattca agtagctgac cttgtactgc
    33601 cagataaaga taccactttt gaggaataca cgaccattga attattatcg gtttctatgc
    33661 tgattgtgat tagtaccttt gcccttgatt ttatccattc ggacagaagt atttttaaga
    33721 ttgtagacct tgacgaagcg tgggcgttct taaatgtggc acaaggagaa acgctctcta
    33781 acaagctggt tcgtgctgga cgagctatgc aggcaggcgt ttattttgtt acccagtctg
    33841 cctatgatgt ttcaaaggaa agtctgaaaa acaatatcgg cttgaagttt gcctttcgtt
    33901 ctactgacat taacgagata aaacagacct tagagttttt tggtatcgac aaggacgacg
    33961 agaacaacca gaaacgcttg cgtgatttag agaacggaca atgcttattg caggacttat
    34021 acggacgtgt cggtgtggtg cagatacacc ccgtctttga agaactgcta cacgcctttg
    34081 ataccagacc gcccgtacag agaaatgagg tggagtgatg aaagaaagga taaaaggtgc
    34141 gttcacaaaa aagaagattt tccactttct caaaatggtt ctgttcgtgg tggcactctc
    34201 ccttatcctg ctttcacttc tggggacggt ggctcatgcg acggggcttg tggacgatac
    34261 cataaatgca gaaaatcttt actcaaaata tccgctgtcg aattaccagc ttgattttta
    34321 tgtggataat agctggtcgt ggcttccgtg gaactggctg gacggtatcg gaaaatcggt
    34381 gcagtatggg ctttactgca ttaccaactt tgtctggacg ataagccttt atttaagcaa
    34441 tgccacgggc tatgtggtgc aggaagccta taaactggac ttcattaacg atatggcaga
    34501 cagtatcgga aaaagcatac agaccattgc aggcgtaaca cagaacggtt tttcttctac
    34561 gggcttctat gttggtttcc tgcttctcat tatcttagtg gtgggacttt atgttgccta
    34621 tacgggactg ataaaacggg aaaccagcaa ggcacttcat gccgttatta actttgtggt
    34681 ggtgttcgtg ctgtccgctt cgtttattgc ctacgctccc gactacatca aaaagataaa
    34741 tgaattttca agtgatatta gtaccgcttc gttggacttg ggaacaaaaa tcatgctccc
    34801 aaactctgac agcaagggca aggacagcgt ggacttgata agggacagcc tattttctat
    34861 tcaagtggaa cagccgtggt tactcttaca attcgggaac agcaacgcag aagaaatcgg
    34921 ggcagaccgt gtagaagctc tggtatcggc aagcccagag gacgaggacg gaaagaccag
    34981 agaggaagtc gtgaaaacag aaatcgagga caacgacaac aacaatctga cgataccgca
    35041 ggtcgtgaac cgtttaggta tggtgttctt cctactgttc ttcaacttag ggataacgat
    35101 atttgtattc ttgcttacgg gcatgatgtt attcagccag atacttttta ttatctttgc
    35161 tatgtttctg cctatcagct ttttactttc catgataccg agctatgaaa gcatggcaaa
    35221 gcaggcaatc gtgagggtgt ttaataccat aatgacacga gcaggaataa cgctcattgt
    35281 aacggtggcg ttcagcattt ccagtatgtt ttataacata tccacggact atccgttttt
    35341 catggtggcg ttcttacaga tagtgtgttt cgctggtatc tacatgaagc tgggtgactt
    35401 aatgagtatg ttctctttga acgctaatga tagtcaaagc atgggacgaa gaattttccg
    35461 cagaccatat ctatacttgg cacatagggc tagacgtatg gaaagacggc ttgctggtgc
    35521 ggttactgct ggtggtgttg ctggtgcggt ggctggtagc acgatttcaa acagaaaagc
    35581 gacaaggaac acagctacaa aagatagtcg gggtaataca tcttccagca tgggacagcg
    35641 tgtaggttca aaggtgggtg ctgtcttaga tacaaaaaat aaagtgaaag ataaggcaag
    35701 tgctgtcaaa gagaatatca aggatatgcc gacacagacc gcttatgccg tccactccgc
    35761 aaaggaaaag gcaaagtcca gcgtgtccga cttcaagcgt ggcatggtgc aggaacagca
    35821 gtccagacag tcgggacgta tggaaaaacg ggaacagcat aggcagaata tcgccgataa
    35881 gcgtatggag cttcaaaagg cacaagaagc aaggcaggca cagcgaaagg ctgacggatc
    35941 agcgacaacg ggagctaccc gtccccatga gcgaccagcc acagcttcaa ccattccaaa
    36001 gccgagtgct gaaaaaacgc aggaagtcaa acgccctgcc acagcgacga cttcaaaaac
    36061 aaatgagcca gtcaagacaa cggttatcaa agagcgtccc ttatcttcgg gtacttctga
    36121 taggaaaacg agccagccta cacagccagt acataggcag aatgtagaaa aagtggtatc
    36181 gcaggaaaca cgccagaatg acaccaaaga ccgcaggaca aaagttcagc agacgcaaag
    36241 cgtccagaaa aaccagcaga ccacagagaa aacccgtaac cttgtaatga agaaaggaca
    36301 gaagaaaaaa tgaaactgaa acatattgct attatcggca gtctgtttcc tatcctcttt
    36361 tcactggtgc ttttctttgg ggtgttgatt agtgcggaca gcgacgacga gaacagcaac
    36421 ttttcttctg gcattacggg tatgaactta tccgcagaag tcttgaaaca tcagcctatg
    36481 gtggaaaagt acgccagaga atacggcatt tccgagtatg tcaatgtgtt gctggcaatc
    36541 atacaagtgg aaagtggcgg tacagccgag gacgttatgc aaagctctga aagtctggga
    36601 ctaccgccca attcattaga tacagaaagt tcaatcaagc aggggtgtaa atattttgcg
    36661 tccctgcttt cgtcctgcaa aaatcaaggt atggacgatt tgaatgtagc gatacagtct
    36721 tataactatg gcggtggtta tgtgggatat gtggcaggaa aaggaaataa acacactttc
    36781 aaccttgcag agagctttgc ccgtgaaaaa tcgggtggaa agaaagtaac atacaccaat
    36841 ccaatagctg ttgcgaagaa tggaggctgg cggtatagct atggaaatat gttctatgtg
    36901 gaattagtca atcagtattt aactgttccg caggtgtcgg gagagctggc acaaaaggta
    36961 atgaatgaag cgttgaagta tcaaggctgg aagtacgtct atggtggtag taatcccaac
    37021 acttcctttg actgttcggg actgacacaa tggtgttatg gaaaagctgg tatctcctta
    37081 ccgagaacgg cacaagcaca gtatgatgct acccaacatc tcccactctc gcaggcaaag
    37141 gctggggact tggtattttt ccattctacc tataacgctg gttcgtatgt aacccacgtc
    37201 ggcatttatg taggaaataa ccagatgtac catgcaggcg acccgatagg atttgcagac
    37261 ctaagcagta gttactggca acagcactta atcggtgcag gacgagtaaa acaatagaaa
    37321 ggacttgaag atatgtttaa gaaaaataag aaacagacag aaactatcaa agaaccaaaa
    37381 gaaaaaaagg tgcgtactgt caaggtaggc acacataaga aaactgtgat tgccttgtgg
    37441 gtggtgctta tcgcaagcgt gagcttcggg gtgtataaga attttaccgc tatcgaccag
    37501 cacaccaccc atgaaaaaga aatcattgag cttcgcttgc aggacaccaa cgggatagaa
    37561 aatttcgtga agaactttgc gaagtcatat tacacatgga ataacagcaa agaagcgatt
    37621 gaagcaagga cgcaggcaat cagcggttat ctgacaaagg aattgcagga cttgaatata
    37681 gataccatta gaaccgacat accgaccagc tccacagtta cagatgtgat tgtatggcat
    37741 atcgagcaat cgggaacgga tactttttct gctacctacg aagtagatca gcagataaaa
    37801 gagggcgaac agacaagcaa tgtgaaagca acctatactg taaaagttca tgtagacgct
    37861 gacggggata tggtaatcgt tcagaaccct actcttgcac cagcaatcga aaaatcagac
    37921 tatgatccaa agacaccaga aacagacgca agtgtagacg ctgatactgc aaatgacgct
    37981 accgcatttc tggaaacatt ctttaagttg tacccaacag ccacagaaaa cgaacttgct
    38041 tattatgtgt cggggaatgt gcttgaacct atcggcaggg actaccttta ttctgaactg
    38101 gtaaacccta tctttacaaa agacggggag aatgtaaaag ttaaggttgc tgtaaaattc
    38161 cttgataatc agacaaaggc aacgcaagta tcgcagtatg agcttgtgct acataaggat
    38221 agtaactgga agattgtagg ataattgtaa aaggcttgta tcatattttt atgctctgca
    38281 agccttttaa tattgacaac tgtattttta agtattatgc tgtacttgta taagctatac
    38341 agtatagaac aaggcggtgt ttaaatggaa atctttatca ataatcatgc aagcatacct
    38401 atttatgaac aaatttcttc gcaaataaaa gcattgatta taaatgggga attaaaggct
    38461 ggcgaagctc taccatcaat aagaggactt gcaaaatcat tgcatataag tgtattgact
    38521 gttcaaaaat cttatgatgt gcttcaaaaa gacggtttta tcgaaacaac agcaggcaaa
    38581 gggtgttttg tatctgctca aaatcaagat ttttatatag aagaacaaca gaaaaaaatc
    38641 gaagatagat ttgtagaagc tattgaaata gcccgtgcaa gtggcataaa ccttgataaa
    38701 ttgattggat tattaaaatt attatatgag gaggacgagt aaatggctga aaatatttta
    38761 gaagttaaaa accttacaaa agattatggc gattttgtat taaatgactt aagtttttcc
    38821 attcctagag gtgtaattat ggggcttatt ggcgaaaatg gagctggaaa atcgaccaca
    38881 gtaaattgta ttttgaacga aattaagaaa aacagtggaa acattttgat ttttggaaaa
    38941 gaccacataa ttgatgaatg tgaaataaaa gataaaatcg gaattgtatt tgacgaaaac
    39001 cactttccag atatttttac accaaaagaa attgaaaaat atatgtcggg aatatatacc
    39061 aattgggaca gtatagcatt tcgtaattat ttgaaacagt tcgaattacc agcaaagaag
    39121 aaaattaaag atttttctaa aggaatgaaa gtaaaacttg cattcgctgt tgctctttca
    39181 cataaagcag aattacttat attagatgaa gcaacaagtg gattggaccc tattattcga
    39241 gatgatattc ttgatatact gattgatttt gtgcaagatg aaaattactc tattttgttt
    39301 tcatcacata ttacaagtga tttagaaaaa atagcagatt atattacttt tattcataaa
    39361 ggacggttag ttttctgctc attaaaagac gacttaattg ataactatgg tgttgtaagt
    39421 tgtggagcaa gtgtctttga tacgctagat aagacagaaa ttatttccta tcaaaaagaa
    39481 gattatcaat ataaggttct tgttcgtgat agaaacaaga ttgcaaaaga ttttccaaaa
    39541 gcacttgtag aacctgctac tattgaaaat attatgttgt tttacgtaaa gggggaatta
    39601 caatgaaagg cttattgatg aaagatttat tagtaattca gaaaaaatat ggaattacta
    39661 gaattattat ggatatattc attatagtag ctttgatgtt atttttaaaa gggacaggag
    39721 ctatttacat tagtttattg ttaattccag ttgaaataat gacaatgata atttccttaa
    39781 cgacttgcga cgaacaatgg aaatggggga aatatgttgt tgcactaccc gtttcaaaaa
    39841 ctacgattgt aaaaagcaga tatatagttg ctttgattat gacttgtatt ggctttgggg
    39901 tagctttgct tgttaatacc ataacatatt ttatattccc tcaatttgca tttggttatt
    39961 atatgtttat tgcttgtgct tcttttgccg taaccttagt atttttgtca tttgtattgc
    40021 cttcaaatta ttcacttgga gttaatgcag gttttgcggt tatgtttact ctagtgatta
    40081 ttcttgttgt attggcagtt tggacaaatg tgactaacaa ttctatttta tggtttgtag
    40141 tcgataattt tgaattgagt atcggcatag cttttataat agttatagct cttgctttca
    40201 gttcttatct tctatcaaaa gcatttttta ctagaaaata caattagtga ggtattgtta
    40261 tgagaaaatg tcttagatgt catgaagaaa tggtcgaaaa tttagatgtt aaagttgata
    40321 tgcagggata tggtattaga ataactacta aaggcgtttt tggaactacg gtagaaaaac
    40381 ctaaagttgc agtttgtcca aaatgtggcg aagtttcctt atatattgaa aattttaaat
    40441 caataaaata aatgattaaa atctgcatat agactgaggg tttattgttt gctaacgtcc
    40501 gagcggatat tgaagaattt atgaatagta catacccaga gatgcaagca aatttcaaaa
    40561 ggtattttgg aaatagacat tttacgccga aaaaatatgt ttatatcact acgaaaaagc
    40621 aaaaattaaa tttcttaaaa gaataagggg gggctatgga taagcaatta tgggtattat
    40681 gtcctatttg caataataag acacgaacta aagtgcgaga agatacagaa ctgataaatt
    40741 ttcctttgtt ctgtccgaag tgcaaaacgg aaactttaat caatataaag catggaaaag
    40801 taactattat caaagagcca gacgcaaaga cgcagagccg ataatttgta ggttattatg
    40861 acttatagat tgttggctct tatttgttac ctgcggagca gatcagcaaa tagataatgc
    40921 agaatagcaa aaagggaaaa caagaagctg attacttctt gctctccctg ctgttcaaga
    40981 taccgtcaat cgtaccttga atgattttta attcgttatc actcaataaa tcaagagagg
    41041 tttctatctg acggcgagct gtacttttta cgacgttctc tgctggatag aaatattcat
    41101 cgaccgatat attgaacatg gtaactaaat catggaataa ttgaaaacta gggtgtgaac
    41161 caacattttc aatatctgcg atatggcgtt ctccatagaa cactttatcg cctaaatcat
    41221 tacgagaaag ccctgctctc tcacgggctt ctctgattgc cagtcctaaa ggtctgaaat
    41281 caaaagtttg tgtttctttt gcttttctca ttttaaatca cctagcatga gtttatactt
    41341 catgctgtat atttagaatg tgttaaaatt gagcgaatca ttcaattaga aaacgaaaaa
    41401 gaatagaatc acaaatcaca agtgattaat cacaaatcac ttgtgatttg tgattgttga
    41461 tgataaaata agagtaagaa gaaatagaaa gaagtgagtg gttgtgggaa atttaggcgc
    41521 acaaaaagca aaacgaaatg atacaccaat cagtgcaaaa aaagatatta tgggggataa
    41581 gacggttcgt gttcgtgctg acttgcacca tattataaaa atcgaaacag caaagaatgg
    41641 cggaaacgta aaagaagtta tggataaagc cttagaagaa tatattcgga aatatttacc
    41701 tgacaaactt taaaaggagt gaaaatgaaa tggcagttac ttatgaaaaa acatttgaaa
    41761 tagagatcat taacgaatta tcggcaagcg tttataatcg agtattaaac tatgttttga
    41821 atcatgaatt aaataaaaat gactctcaat tattggaagt caatttatta aaccaattaa
    41881 agcttgcaaa acgtgtaaat ctttttgatt attctttaga agaattacaa gccgttcatg
    41941 agtattggcg gtcaatgaat cgctattcaa aacaagtttt gaataaagag aaagtggctt
    42001 aatatggcaa atatagtcaa ttttactgac aaacaatttg aaaatcgctt aaatgataat
    42061 ttagaagaat tggttcaagg aaaaaaagcg gttgaatcgc caaccgcttt tttacttggt
    42121 gggcaaccag ggtcagggaa aacgagtttg cgaagaagat gattgagcag gcaggaacaa
    42181 agtgataaat tcaaattgtg agggaaaata tagatgaatc aaggaagaat tattgtgatt
    42241 acaggctcac cggggacagg aaaaacaaca actgcgtcaa ttgtcgcaaa ggaatcagat
    42301 atggataaat cagtgcatat gcacaccgat gacttttttc attacttaag caaaggcgca
    42361 ataccaccac atttgccaga atccaacaag caaaatctgg ttgtcattga agccttttta
    42421 gaggctgcaa agcgatatgc tcgtggcgga tatgatgtaa ttgtagatgg gattgtcggg
    42481 ccatggtttt tggagccatg gaaagctctt gcccaagaag attacgaggt acactatatt
    42541 gtattaagag cgagtatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg
    42601 cagtatctta aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta
    42661 attaagaagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
    42721 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
    42781 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
    42841 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
    42901 tactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
    42961 gtataaaatt gttgggagta ttccttacca tttaagcaca caaattatta aaaaagtggt
    43021 ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
    43081 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
    43141 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
    43201 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
    43261 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
    43321 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
    43381 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
    43441 ttggaaagtt acacgttact aaagggaatg gagataaatt attaggtata ctactgacag
    43501 cttccaagga gctaaagagg tccctagcgc ctacggggaa tttgtatcga taaggggtac
    43561 aaattcccac taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata
    43621 ttgtattaaa aaaatcgttt tatggttgtt caaattatcc tgaatgtaag tttactttag
    43681 ctgaacattt tagaaagaaa aaataaacga ggagtatgta aatgagatca gaaaaagaaa
    43741 tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt gtgacccttg
    43801 aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat gatattacat
    43861 attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat caatttggga
    43921 atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa gaaaagggat
    43981 tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta ttgcccttgg
    44041 aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt gataaagatt
    44101 gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga aagccaagcg
    44161 caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat gttattaaag
    44221 gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt gttcgccatg
    44281 agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag
    44341 gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag aaacttttgt
    44401 ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta tgccatcaat
    44461 tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat ccggagtatg
    44521 ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt aaaaccggct
    44581 gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga ttacagaaat
    44641 gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa
    44701 gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc
    44761 atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga
    44821 atatattgac aatactgata agataatata tctttactac caagacgata aatgcgtcgg
    44881 aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata tcgccgtatg
    44941 taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta tagaatgggc
    45001 aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg
    45061 taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
    45121 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
    45181 cagtgaattg gagtctggtg tggtataaaa aaagttgaca tcttattctc aaggatttca
    45241 tgatagaatc aagagaataa ggaggtactc aaaatggcac gtaaatatga ccatgaatac
    45301 aaagtacagg cagttaagtt agcaaaagaa ataggtggtg ctaaagctgc taaagaatta
    45361 ggtatcccgg agggaactat ccatacatgg ctgaaagcag ttagagcagg cacattggat
    45421 attggcgatg gtgcacatac tccagaaagt gcgatgagtc ttgctgagga gcttgcgatg
    45481 ctccgcaaac gcgttaagga tcaagataaa gaaatccggc gtctgaaaga ggaaaatgaa
    45541 tttctcgagg aagcaagtgc ttttttcgca gccagccgtc ggaagtcagc aagaaccaaa
    45601 gaatgaagtt cattgccata aaaacggaag acggcgtgat taaggggaaa ctctcattct
    45661 actgccggat gctcggtgtc agccgccagg gtttttacaa atatcttgct attaaagatc
    45721 gtccctggaa atatcaggat ctcgctgatg ctatgagagc gattaattct gaggatgaat
    45781 gcaatgatac ctatgggcgt attcgcatgt atcaggcact tctccttaag aatctggaag
    45841 gactcaaaat ccccagtgag cgaactgtct acagggttat ggatgaaata ggccttagcc
    45901 atcggccaaa gcgtaagcca aatggtatta ccagagctga tcgggaagcg cgtaaatcgg
    45961 atgatcttct gaagcgagat ttcaaatcca ataagccact tgaaaaatgt gtaactgaca
    46021 tcacagagat caaggctaaa gatggaaaac tgtatgtttc agctatcttt gactgctttg
    46081 attccagtgt ccttggtctg gcgatggaaa ccaacatgaa agcaactttg tgtgagcata
    46141 ccctggataa tgcctatctt gcatatccta atctgcgagg cgccatttta cactctgaca
    46201 gaggaacaca atataccagt gaaacctatc gtaaggctct ggcaaaatac ggtattattc
    46261 aaagcatgaa cagtgctggt ggcaggtgcc atgataatgc ccgatgtgaa agcatgtggg
    46321 ccagaatgaa aagcgagctt ctctatgacc gctacaatac ggaaactatg accacagagg
    46381 aactgaaggt tctcatttgg agatatttca tcagttactg gaataacagg aggatctgct
    46441 ctgctaatgg tgggcttcct ccgatcatca agcgccagag atactatcaa tctttggacc
    46501 tggctgcata gtcagtgata tctttgagat aaatgtgtca accaatattg acaatatcag
    46561 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
    46621 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    46681 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    46741 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    46801 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    46861 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    46921 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    46981 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    47041 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    47101 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    47161 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    47221 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    47281 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    47341 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    47401 ttttactgga tgaattgttt tagtacctag atttagatgt ctaaaaagct ttaactacaa
    47461 gctttttaga catctaatct tttctgaagt acatccgcaa ctgtccatac tctgatgttt
    47521 tatatctttt ctaaaagttc gctagataga gttctatatt agagatgtaa agagttttgg
    47581 tgaagaggtt tgtgagtatg gcttttataa ccagggtgca atgagaagca ctaacagcag
    47641 ctagcttaag aacgcatagg atatactttt tatggaagtc acagaggagg aataaaattt
    47701 gaacaggatt aacagagtaa cctctattct tattcagctg caatcaaaaa aaataattcc
    47761 tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca gagatatccg
    47821 gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat attttcttgt
    47881 agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg cattgattac
    47941 agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt ttgattcagc
    48001 tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac aggagttgga
    48061 gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg ataacgttat
    48121 tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat atcctgcatc
    48181 aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt tttatgaaca
    48241 gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt tcgtgtggat
    48301 cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg ctctttggaa
    48361 caaatattaa aacaaatgct ttcatataaa aaactatata atgtaattct tagagctgaa
    48421 aaaggggaga catacaattc tatcaagaac aggtattctc tcggcttcct tgaagaaacc
    48481 gatttgggaa gtaaaatgga aatcgaattc cagacagatt cctttgagat attgagcaag
    48541 cagctgattg aatatggatc aggcattgaa atagtccaac ctgatgaatt aaaatgcata
    48601 acccgtaaac acctggcaca aattacgaat cactgcctaa acttgattta gagaaagcga
    48661 ttggtctgtt atgtattttt tgtaaggttt catatcccct gctgacatac agctgtcagt
    48721 gtattatgat attctactat caaaaaggag gattgaaaaa tatgaaacca aagataatta
    48781 tgcacacaca aattagcctt gatggccgca ttaaaggctt tgataatccg gaagtttact
    48841 accaggttgc cgggggaatt cattcggatg cggtgctgtt tgggtccaat actgttttta
    48901 ctgcatttga aaaatatcct gctgaaacag aggctgattt tgagaaaatc ataacaagcc
    48961 cggaggaccc cagaccaata ggagtcatac ctgacagcag aggtatcttg agaagtcttc
    49021 attgcttaag aaatcttgga tatctgaagg aaattgtcat tctggtatca actgccactc
    49081 cgaaggaata tttgaattat ttggaggaaa ggcattaccc atatattgtg tcaggaaatg
    49141 atcatgtgga ctatgaaaaa gcctttcaga tcctgcatga gcaatatggg tgtaaataca
    49201 tgcggacaga cagtggcggt ggattaacca acaaattatt agaaaacgga cttattgatg
    49261 aaatcagtct tgtgatatca ccatgttttg taggtaataa agaaaaacaa ctatttgata
    49321 accttctgtt atctgaaaaa gttaatttgg aattgcaggg aacagagaat gcagaacatg
    49381 gctgtctgtc attgcgctat gctgtgaaaa ataaggatta gtgcagcata ctttaaaaag
    49441 tgtctgttgg gacagtgtcc taaaggaagc ataagctcat ggctagcggc ggtgttaaag
    49501 taacggcact cggccccaat atatagttga tatgccccta agcctttgag ggcaaggcgg
    49561 tcaaccgcgc cttttccggt ccgcatacag tcgttcaatg agggtatcaa gctcggcaat
    49621 gcggcggtgc ttctcggtgg ttcgtttctt cgccgccgtt ttcggctaat tctaattgat
    49681 ccgaactgta aggataagca attatccggg gctagttaag tatcttagag aggaagataa
    49741 agataagtaa accaatttta aagttgtacc gaataaagcc ttcaagagga caacatgctt
    49801 ttgaaggctt tattgcttac aattacttgg attgtaacgc aaaagcaggc accaaatcaa
    49861 tgaaaaatag cctatccagg cgactgtttc agaaatatgg gcttccgcca aggggatacc
    49921 agtggggtgc gacatctttt ttgcccactc aaggcatgtg gagtgtgtgg cgttgttgcg
    49981 tagaaagtaa ggctggagtc cttatatctg gctgagctta atctctggta attttgaggg
    50041 ggctgaccca ttagtccctt ttgaaccaaa aaatcgaagg cacccgtgaa agatgtcttc
    50101 gatttttgtt ttttttggat gaaatataga ggattttccc cctatatttg gttttgtatg
    50161 attgtttttc cctcatattt ccttactcta gttactaatt tcctaaaatt gtgcgcaaga
    50221 aatacaagtc ccatttcctt aaaaattttt tcagctcctc ggacatggaa tcttttaaat
    50281 ttcatatttt gtttaatgtt tccaaaaact gtttcgacat ctatctttcg ctgcgcatag
    50341 tatgaggctg tttctttgct ttctatcttt tcattcgctt ttatttttat atattcccag
    50401 gttggattaa tattcaaacg tcgatttttt ccttcttttg ctttggtaca tttctctctc
    50461 aatggacatc ctgtacaatc ttcacattca tagattttga agtttcgaat aaatccattt
    50521 ctatctttac gttgtgaata atgtgagaac actactcttt tctgattggg acaaatatag
    50581 taatcatcca actcgttgta agtccaatta tctcgatgaa actgattttg tttatgggcg
    50641 cgtttcttct cttttaagta accttgatgc ggaatgatgg gggtggattc tgtttcgtct
    50701 aacacataac aataattttc ttcacttgca taaccggcat ctgcgacaat attttctggt
    50761 aaacctattt ttgattcaat cttttcaaga aatgggataa atgttcgtgt atctgttgga
    50821 tttggataac aatcataagc aagagcaaat tgattttctg tcgcaagttg aatattataa
    50881 cctggtttta gttggccatt aagcatgtga tcttctttca ttcgcataaa agtagcgtca
    50941 tgatccgtct ttgagaaact attgcgtaca cccataatca ctaattgttc ttcgtagctc
    51001 atttttcttt gggcaaaatc atcgaatcgc ttcttctgct tatgaacttc agatttttct
    51061 ttacgaagta attttcgttc ttctactgtt tcggtttggt caatcgcttc aattaattga
    51121 ttttcctttt cttccaaaga ttgacgaatc ctttcaatct cttctataga aagttcatcc
    51181 tttaaatcat ctattaactc aggtaagatt tttgtttcca ccatttgttg gtagttttca
    51241 agggcttttt gatcaaggct atccttatac tttaaaatac tttttttcca aacgaaagag
    51301 tatttattcg catccgcttc aatttttgta ccatcaatgt atagagcact gccactgatt
    51361 aagccacttt gaaccagtag ctcacgaaat tgaacaaatg aatgatctag aatatcgttc
    51421 ataatcggat gaacgcgaaa acgattaata gtacgaaagt tagggaattg ctcttgagat
    51481 aagtagcgca tccgaatact atctatcagc atattttgta tttggcggcc cgaataaacc
    51541 ccttgaatat aggcacaaag aataatggaa agtagcatac gaggatgata ttggggtcta
    51601 cccatagatt gataataagg aagaaagact tcatcgggaa tagagtcaac aaactgcaca
    51661 atggcatgag caaaatcatt ttcttctaat ttaatttcaa gatctatagg caaacaaagt
    51721 tggtttatgg tataattttg atacatgata aagcacctct ctcaaatgga ttaatcacct
    51781 atattatagc agagtgtgct ttatttttgt attcactttc aaaagataaa tagaggtctt
    51841 tccactacta tctaaatgga aagacctcta tttttcatat aggagctact ttttggtcag
    51901 ccctatatat taatcacaaa tcacttatca caaatcacaa gtgattaatc acaaatcact
    51961 tgtgatttgt gattcctaat gatacaatat tactatacaa aaaaagaatg gggcgtagtt
    52021 atggagaagg aagaactcaa aatacttgaa gaattaagac gtattttaaa cagtaaaaat
    52081 gaagcgattg ttatcttaaa caattatttt aaaggtggtg ttggaaagtc aaaattatcg
    52141 actatgtttg cttacttgac agacaaattt aatttaaaag ttttaatgat cgataaggac
    52201 ttacaagcaa cattgacaaa agacttagca aaaacattta aggtagaatt gccacgtgtt
    52261 aatttttatg aaggactgaa aaatggaaac ttggcttctt ctattgttca tttgactgat
    52321 aatttagact tgatccctgg cacgtttgat ttgatgttac tgccaaaatt aactcgctca
    52381 tggacttttg aaaatgaaag tagattgctt gctactcttt tagcaccttt aaaaagtgac
    52441 tatgatctca ttattattga tactgtacca acgccaagcg tttatacaaa taatgcaatc
    52501 gtggcaagtg attacgtcat gatcccttta caagcagaag aagaaagtac aaacaacatt
    52561 caaaactata tttcctattt gattgattta caagaacagt ttaaccctgg actagatatg
    52621 atcggttttg ttccttattt agttgatacg gacagcacaa cgataaaatc aaacctggaa
    52681 gaactgtaca agcaacataa agaagataac ttggttttcc aaaatattat caagcgaagt
    52741 aataaagtaa gtacctggtc taaaaacggc attacagaac acaaaggcta tgacaaaaaa
    52801 gttttatcta tgtatgagaa cgtatttttt gaaatgcttg catatagtat gcagtaataa
    52861 ttcaaaagct gttatcgtgt gcggtaaata aaaaaaaccg ttcctagata atacactttt
    52921 gtgcgtcttg aaaaataacg ggacggtttt ggatataacc agaactgtcc ttttttctta
    52981 ggagtaaata aggcatagct ctggttaaag ggggctatac catgattgca tacggaagaa
    53041 cgttctgccg ttctcaactg aataaatatt tcagtccact attacaatca ttacaccctt
    53101 tacaagaatt aaaaattcat atcaatagaa tttataaaac agtctgtttg tgtaattaca
    53161 cattatcggg ctgtttttgt tttgttaaaa atttttttca acttttttcg gtttcaactt
    53221 agcaaatcac acctgtcgtt ccctatatgg tgcggaaaga gggataacca cttttcatat
    53281 cgtaacggaa agggggtgag attgatgaaa ccatctgact tccagaaaac agttcaatgc
    53341 cgttttgaaa gttgtttgaa gaaagttgtc cgacatgttg ttaaggacta tcagaagaaa
    53401 ttaaagcgac gacaaaaggg agaaacgctt ttttgtgaac ttccagaaat cgttgtagaa
    53461 aatctggctg tctgggacga ctatgatacg gactatacga ttttcaatgt atgtggtagt
    53521 gatattcgta tttgtgatga tgaactggca gaagccttga aacatttgtc tgagcgtaat
    53581 cgagaaaatt tgttgatgta ttatttcttg gaaatgagcg ataccgagat tgcaaaaaga
    53641 caaaatattt caagaagtgg tgtttttcaa aaccgacaca attcactaga gcttatgaaa
    53701 aaaatactaa aggagaaatg atagcaaatg aaacacacaa tgaagaagcc gtcctactac
    53761 ttgctttcgc aggcaatgga cggggacgaa aaagcgattg aaaagatact ggcattttat
    53821 gacccttaca tatcaaagtg ctgtctacga ccactctatg atgaatacgg caatgtctat
    53881 attgttgtag atatggaatt aaagggactt atcagagaag cacttattaa aatgatttta
    53941 gggtttgata ttgccttaga aatcgaagaa gaataacaag cagtaaccgc agagcatgat
    54001 ttgttcaatc ccctctttcc tgctctgccc ctgcttttat atgaaagcaa cacgctttcg
    54061 ccacagctct ttgacaactg aataaagcag acagatacgt ttgtgagata gcgagccacg
    54121 ggaactgtac gccacgacct tttcaaaaga aagcgagcga cccacgcagt gatccgagag
    54181 cgactgttgg aaaagtcgtt ttgccatgac ctatcctcac agaataatga tacgtccgca
    54241 tggtgcggtt ctgcccacaa gaatgggaat agttgagata ctaacggagc tttccaaagc
    54301 cgtctgcctg cttataattg atagtttcag caacaaaatc tatctactct ttttatttag
    54361 gtgtgttata atgaccttat tagacgagtg tattttgttg ctgtcacaaa tgaaaggggt
    54421 cttaatagtg ataagtgata cacaaaatgc acaatctcct acaaaggaaa aaaagactta
    54481 tacagtagag gaaattgctg aacaactgga cattagcaaa aaggtagctt attcattagt
    54541 aaaaagcggt caattcagtt atgttcgtgc tggtaaagcc atacgggtat caaaggtttc
    54601 tttcgataaa tggttaaatg aaatttaact ataaggagag aaaattatgg cttcaatcaa
    54661 acaaagaaaa tcaagttttt cagttattta ttggtactta gatagtgcag gagaaagaaa
    54721 acaaaaatgg gacacgctgg aaacaagaaa agaagcaaaa cagcgaaaag cctttataga
    54781 atactatcaa gagaaatttg gatatgtgat agttccatta gaggaacaat ttgcacatca
    54841 gattgaaaat tctaaaaagg agttagatgt tgtagacaaa gatattacat tgcgtgattt
    54901 cctagaaatt tttgttaatt tatatggcac atctaaatgg tcgccgagta catttagtag
    54961 taaggtaggc agtattgaaa attatatcaa tccgattatt ggagattgga aactgaatga
    55021 aataactaca aaaagtctat caaaatacta caatgattta ttgagcgttc cagaagtacc
    55081 acgggcaaac agaaaagcaa ctggtcgttg tgttcaacca gcgaacatca aaaaaataca
    55141 tgatattatc cgctgtgcct taaatcaagc tatccgctgg gagtatattg atacaaacaa
    55201 aagaaaccct gcgtctttgg ctacgttacc aaaaattcca aagactaggc gaaaagtgtg
    55261 gagtgttcaa acatttcgag aagcggtaaa agccacggaa gatgatttgc tttcaatttg
    55321 tatgcactta gctttttcct gctctatgcg aattggagaa attacaggac ttacatggga
    55381 agatgttatt attgatgaag agtccattgc tacaaataat gcaaaggtaa tcatcaataa
    55441 agaactttca agagtaaatg cagaagctat gcagaaatta aaggaaaagg atattttaaa
    55501 gattttccca acgcaaaaac cgcattgtac cacaaggctt gtcttgaaaa cgccaaaaac
    55561 agaaacaagt aatcgtgttg tctggttacc taaaacagtt gcggaactat tggtgcaata
    55621 taaaaaagac cagcaggaat taaaggaatt tttaggaagt gcctacaatg attacaacct
    55681 tgttattgcg ttggataatg gcaatcccgt ggaaagccgt attgttagag ataggtttca
    55741 aaagctctgt gaagaaaacg attatgaagt tgtggtattt cacagcctac gccatttaag
    55801 tacgggttat aaactcaaaa tgacaaacgg agatgttaag tccgtacaag gcgatactgg
    55861 tcacgcagaa gcagaaatgg ttacagatgt atattcagaa atcattgacg aggacagacg
    55921 tttcaatgca caaaaaatgg ataaagaatt ttattctacg cttaatgatg atacagacaa
    55981 tccaaagcaa gataccgact taaccgatag tgatatggcg ttattggaac tgattaaatc
    56041 tttatcgcca gaaatgaaag agcaattttt aaagcaggca cttcaaagat agaatttcaa
    56101 aagtgttagc aaattccaaa aaaaccagcg atttttcttc aaaaataaaa aagtgttagc
    56161 aagcctattt ccttaaaagt cttaaaaaaa tatcgaataa aaaatgacag attgaaaaca
    56221 attcgtattc agcgtaaata gaaagccata aataaagaaa cgctgaaaac cttgtaaaat
    56281 aaggctttca gcggagtttg tggcgtccca gacaggagtc gaacctgcga cgtttcgctt
    56341 aggaggcgaa ccctctatcc tgctgaggta ctgggacatt cttaaaaatc tattcaattt
    56401 tatctagtta tcatatcaaa aggcgtttgt tccacctagc ccttaggagg ggatagtcac
    56461 gcaggaggaa tttgatattg tccaacagct tatgacgagc cgcagacggg aaaagaataa
    56521 gggcggcttt gagaatattt tttcaggcgt tatcaaatgt gcggactgcg gctatgctct
    56581 gcgggctatg agtgcaaaca ggaggaaacg ccccgacatc atcgactgca tacaatacac
    56641 ctgtaataat tatggcaggt acggcaacgt catgtgtacc gcacacgcca ttgaagcgag
    56701 ggacttattc aacgccgtcc ttgcagacat caaccgtttt gcggatatgg cggtgaatga
    56761 cgagcgggcg gtaagggcga ttgagaagcg gctcacggaa acagaccaga gcaaggcaaa
    56821 ggcgatggag aaagaacgaa agaagctgaa taaacgcctt gcggagcttg acaggctgtt
    56881 ttcctctctt tatgaggata aggtcatgga gcgtatcacc gagcggaatt tcgagatgat
    56941 gtcggggaaa taccagaaag aacagcttga aatcgaagcg agattaaaag aggtaacgga
    57001 aactcttaac gaaagctatg agaaatcgca gggaatccgt gacttcctct ccttaatccg
    57061 caactatcaa ggcttaaagg aactggacgc aacggttgta aatgcactga tagacaagat
    57121 acttgtttcg gagcgtgaga aatcagcgga cggaacagtg aaacaggaaa tcaagattta
    57181 ttataaattc atcggctttg tcggtgaatt acatatcaca cctacaaaac ggtggacagc
    57241 gttacccgct aaacactgta cggtgtgcgg cattgaatac gtccccggct ctggcatatc
    57301 aaagtattgc cctgcttgtg ccaagaagat acagagggag aagtcaaacg agagcaaacg
    57361 caggagcagg gagcagaaaa ggatggcatg tattgaactg tccgcaaaaa atgaccgact
    57421 gatatggtgc agaaagaggg atacatacct ttcacttcat agagaaaggg ggtatgattt
    57481 tgaaaccatc agacttccaa aagacgatac agtgtcagtt ggactgtaag ctcaaaaagg
    57541 ttgtgaaagg cagtgtccgt aactaccgca aggaattagc cagatgacag gcaaaggaag
    57601 tacccttttg tgagcttcca gaaattgtta ttgagaaatt gattgtctgg gatgattacg
    57661 aaagtgacta tgcgacattc gatgtgtgca gtacggaaat ccgtgtgctt gatgaagaac
    57721 ttgaaaaata caggatatat cggcatttac caacttatga aaagataatc agaaatttag
    57781 tgtattttta taataaaaat ataatgtttg tatacaaaaa tattaaagac attttagtaa
    57841 gttttgtata taagcaaaca tgtatttttt aatatagtta tctaaatatt attttatata
    57901 caataaaata ggactcctaa aaataaaaaa tatatcataa atataaataa gtagatatag
    57961 gttttaattt gatttataaa cctattataa aataaaattc atgattttta taagcaataa
    58021 aatttttagg aggatatatg atgaataaaa gaatgaaact aattccatat gaaataaatg
    58081 aaaatctaag aggtgcaaaa aataaattcc catatggaat aaaacaaatg aatgctaggg
    58141 gaatgtggga tgaaggttat actggtaaaa atattgtagt tggtataata gatacaggtt
    58201 gtgatatatc tcatcctctt ttaaaaggaa aaataattgg tggtgcaaat tttagtgatg
    58261 acagtaatgg aaataaaaat atatatgagg attttaatgg tcatggaact catgtggcgg
    58321 gtattatagc tgcatctaat tataataatg aagttatggg agtagctcca gattgtaaat
    58381 tattaatagc aaaagcatta aataaagatg gtaccggaac atatcaaagt ataattaatg
    58441 ctattaactt tgctgtaaat aacaaggttg atattatatc tatgtctctt gggggaaaca
    58501 aagatgataa gaatttaaaa aatgctgtca tgcaagcagt aaaaaataat atttctgtag
    58561 tgtgtgcagc aggtaataat ggagatggtg attctagtac aagtgagtat agttatccag
    58621 ccagttatgc tgaggtaata gaagtaggtg caataaatga aaactatttg gttgaaaagt
    58681 ttagtaattc aaatactaca atagatttgg tggctccagg aagaaatatt atatcgactt
    58741 atatggataa taaacttgct attatgagtg gtactagtat gagtgcacca tacgtatcag
    58801 gctcattagc actaattaaa gaatgggcaa gagaggagtt tgaaagagat ttagatgaag
    58861 ctgaactgta tgcacaatta ataaaatgta cgagagcgct tggaatacct agaacggaac
    58921 aaggaaatgg atacttatat ttaaatcttt ataaatacaa gaataatagc aaaagataat
    58981 ttttgatttg atgataaaat agctatatta tatagagtcg agacaaataa taaaaattac
    59041 ttaggtgtaa gattttacat caatgtataa aggtattaag tgataaaatt tataaacata
    59101 ttagctagtt agaattgaaa aataaatata cgatattata gatagcacat ctggaaaagg
    59161 tgtgtttttc tatgtactta tacatataaa agatttataa gagatgcaaa agtactataa
    59221 gttagaaatt tttctatgaa agattagaaa tacgatacag tgttttgata ttataaaatg
    59281 aatataaagt ataaatgata aatgttatca ataagtattg aatttcaaaa tcaattgtga
    59341 tattctatat ttagaaaaat aaagagattt cagataaaga aatcttttgt tttttagatg
    59401 ttagttagct ataactaact attgattaat taaaaaatac tacaggaggt aaaaatgagt
    59461 aaaatatata ttgtttattg gagtggaaca ggaaatactg aaaaaatggc aaattttgtg
    59521 gctgaaggtg tgaagttaaa aggtaagaca ccagaagttt tagatgtgag cttactgaaa
    59581 ccaagtgatt taaaagaaga agataaattt gcattaggtt gcccatctat gggagcagag
    59641 caactagaag agggggatat ggagccattt gtttcagaat tagaatctat ggtatcaggt
    59701 aaacagattg gattatttgg ttcatatgga tggggaaatt gtgaatggat gagagattgg
    59761 gaagaacgta tgcaaaatgc tggtgctaca attattggtg gagaaggaat tacagcaatg
    59821 gaagacccaa atgaagaagc aaaagatgag tgtatagaat taggcaaaac gttagctgaa
    59881 taaatttgta tattataaaa atagtataaa tagcaactaa tgatgatagc agtatataat
    59941 aataagagag actaatatta tgatacataa gaaaatatcc ttaatagaga tagataaaat
    60001 attagtctct tttaatataa aattagattt ataatttaat gttgtttttg aattggtaca
    60061 atctcattat aaaaagataa taattgattg ataacataag tgataaaggt agtatcttgt
    60121 gccaaacaaa gcatatttgc tccaatatta acccattctt caatactttt cttttctatt
    60181 gtaggaaaaa taataaaggg catcttatat tttttgcata tttcagcagc atgagaaatc
    60241 atatctttaa aacgccgcca ttggagacaa tgagcggcgt tttggtgtaa tggctaagga
    60301 gaggagggat aggaatgaat atccgagggg caaacagggc aggtgtggcc atacctgcct
    60361 tgcttacaga tataatcaaa gccggtaaag attgcaaatt taccaagggt tccatctagg
    60421 ggaaagtcgc attagtctaa ttaatacctg tctaaccacc aaaccttatt gttttgccat
    60481 aaacataatg ttgcttgaca gaagcaggaa tagcttatat aataaaaacc aacagtcggt
    60541 ttttaataag aaggagttat ataatgacta gaaagaacaa tccaaagcaa gctattgaaa
    60601 atatcattac catttctgca aagttgtttg ctgaaaaagg atacgacaaa acaagtatgc
    60661 aaaatattgt tgatgcttcg ggaatgtcaa agggcggaat ttttcatcac ttcagttcaa
    60721 aagaagatat ttttaatgtg gtcatggaaa ggcgctttga acagataata gagacagtaa
    60781 atcaatggct cggtgaaatg catggactta cagcaaaaga gaagcttaga agtttagtta
    60841 ggcggcattt aacggatgag gtgataaaag aatctagtaa catgattaca agcgcgatag
    60901 aaagtccgca tatcatactg gcatttacac aggataattt gaaaaaacta gcaccaatat
    60961 tagcaaatgt tttacgagaa ggcatagagg atagaagcat catcacagag ttcccagatg
    61021 aatgcgcaga ggtaattttg ctgctcttta acttttggtg tgatacagat gtatttcagg
    61081 gggactttcc tacaattcgt aaacggtttc aattcttaca gtttcttatg agacaaatag
    61141 gagttgatat cctggaagat gagattatag aatcaattaa tattttttat gaaaggaaac
    61201 caatgggaca ggtagagtaa caaaactaaa atagcaacat acttttttta atgttagagg
    61261 agatatctat ggctaaaaaa attcatgaaa gaaggtactt aaaatgatat taatcacact
    61321 ttgcatacta gttgctgttt ttgttctggc gtttgccggt ttgcgtctga tagcccccac
    61381 gattatacgt acaagccata aaatagaagc tcctggaatc gaccgcatgg aaatggtgga
    61441 gatcggcggc atacagcagg cgttatatat ccggggacaa aacgcagaca accccatcat
    61501 cctttgggtt catgggggtc ctggtaattc aatgatgcca tttttacatc tgtaccagta
    61561 tgagtgggaa aatgatttta ctgtggtgaa ctgggatcag cgaaacgtag gcaaaacata
    61621 ctttgcgaac gatccggcag cagttttgca gaccatgagc gcggagcgtg ttctgcaaga
    61681 cgtccatgag gtgacagcat atttgaagca acaattcaac aaggagaaaa ttatccttat
    61741 aggtcatagc tggggaacgg tgctgggaac tatggctgtg caaacctatc cggaggatta
    61801 cagcgcgtac attgggattg cgcagaccgt taatatggtg gacaatgagc gcgtgggcta
    61861 tgaaaagaca cttgaaatgg tgaaagctgc aggaaagcaa aaagatatca aggcgctgga
    61921 agctgttgca ccatatccgc cagcaaaata tggtccaagc tatttggcgg ctcttatgac
    61981 agtaagaaca taccaaagca agtatgggct ttctataaat atggatttgg gcaatataat
    62041 cagtatactg gcaaccccgt attattcatt gagcgagtta aaaaatacaa ccattgtcaa
    62101 tccaaatgcg attgacaatc aaggtgatgt attgcggttt ttagtggaag aagctgacat
    62161 aagaaattat ggagttgact atcaggttcc tgtatactac atcatgggag aaaatgacta
    62221 taatagcccc tatcttccag cgaagaaatt ttttgatgaa atagtggcgc cagacaaaca
    62281 gatttttttt ataccagatg cagaccatct gccatttctt agcaaaaagg cagaatttga
    62341 ctgcatattg ttagaggaaa tcgctccaaa gttagcaaag taacaactag gacagtaacg
    62401 tggatatggt ggcaatttct tttactattc cagaacgctt cagtttgttt ccagcaactg
    62461 gtttcaatga ggtgcttgag atatatctat ttagagggtt ttaagagagg actatcactt
    62521 atggacattt tgtccataag tgacggggtc agcaatcgga aagggtggtc atgctatgag
    62581 tgaactgaga cctttatatc agacagttaa aaaccatgga gtgattatga aaccataaga
    62641 tgattcttgt aatgtacagg agtcatcttt tatatttatt ttctttttta gatttaattc
    62701 tctacttgat ttagatacaa gagaggcttc gccttgtttt aagtatattt ttctacttat
    62761 accattattt cttttttatt tgcttttcca ctttaaataa tacaacttca acacaatttg
    62821 aagctaatat cttttattga ctattgtata aagtattttt ataatgttct tattcatata
    62881 aaattttatt ccacttacta ttgaatttta ttaagaaaaa gatttattac tttctatatg
    62941 atagaaaaat aagtggtatt agttatttct aggaagagaa tatagagtat cctatattta
    63001 ttacaagatt aaaaaaagca ggtttatata ttgaagatat aagaatgctt tcatttttgt
    63061 gatataagaa acaatatgtt tgtgatataa gagacaatat gatagataaa agaatttaat
    63121 tatttcagca atataagcaa gatattaaaa taaaatcatg aactagaaga gtgtatatac
    63181 aaaatagctg agaagataga ctatttaagc aaaaaaggta tgtaaaaaat aatttttcta
    63241 aaagaatcca acagtttttt atttctataa gaaaaataat attaattgga acaatttaat
    63301 aatattcttt cttagatgta tttctatata gtattggaca atgttatata cagttattta
    63361 tatatgcaat aaaaaggaaa attatgataa catatattga tgtaagcagt aaaaatactt
    63421 tcatttatag atataattat tttgggaaaa cactgatttt acaaaataaa aaagtaatta
    63481 tagaatttat caatatatta aaaaactgaa ttgtagtaaa ctaagtgcaa ctaaaattac
    63541 aggagtatta aaagagtatg atgattttat agtaatgaaa gttaatgatg atgatattat
    63601 tttaatataa gaagggaaga aaaaatattt agacaaaaac tacgaggtga gtttatggtg
    63661 gattttattg agtcaaataa tgaagtacaa aaatttgatt ggggagaggt tatgtggata
    63721 cacgaaccat caaaaacaca attcaataga cttagtgctg gaattgtaag attttttccg
    63781 ggaaactgcc aagaaaagca ttttcattta agtgaagaac agcttttata tgtgattcag
    63841 ggagaaggaa tacaaataat tgatggtaaa aaggtcaata taaaggaaac atctatagtt
    63901 tattgcccac catattcaga acatgaaatt ataaacactg gtaaaattga tttagtaata
    63961 ttaattactt atgttcctca taaattttcg agtttgagac aaatacctat agtattttct
    64021 gaaaacaata tacaggagtt agttaatgta aacataattg aaaatcttgc aaatcaaata
    64081 tctaatatat tgaagcttag aatatctgta tatgacttaa agtataaaga aatatttgag
    64141 actaagaaag aaaataaatt ttgcgatatt tgtaaaaata taaaaccgtg tactaaaaaa
    64201 cttgttaaaa gcataaataa tgataacttt gaaaaagtgt atcattgtga gtatggtgtt
    64261 tccagtttag agatacctat agtattagaa gaaaatatag ttggatatat agaatgtggt
    64321 aattttatag tatataagtc tatttatata gataaaaatt tatctgaatt atcaagtagt
    64381 tctggtattg atataaaata cataaacaaa atttacaatg aatttccatt aaatcctaaa
    64441 agtagattat atgtattaaa agaaaattta ttaatgatgt ctgaatttat tcaggaaata
    64501 gctaagagga atttttttga aaatcaactt agtataaaag atgaggagat attgaagagt
    64561 agaaaagaaa atataagact tgaagaggca cttaaaaaag caaatagtag attattagaa
    64621 gaagagtata ttattaatcc gatagatatg ttcaataatt cttcaaaaga atatcccttt
    64681 gaattagaac tttatattga aaaagaaatt aaaaatatga atttagaagg tgtttataat
    64741 ctaatagata caaacaaatt aagatataat gatgttagag atactattca ggaaatgata
    64801 tttgtattgt ctagaacagt gcttagagat ttagaggatt taaaacttat atcttattta
    64861 agaaataaat ataataaaaa aatatcactt gtaaacagtg atgaagattt atggaatgta
    64921 ttatttgaat tttccaaaga gtgtattgat aagaatagag cattttggag aaaagataaa
    64981 ggaaatctaa ttgaaaatat aaacgaatat ataaaaaaat attataaaga aaatataaat
    65041 ttgaatagta tatcagatgt tttttttata agtccaaatt atttgagttc tatttttaat
    65101 gaaagaaata aagtttcaat aactgagtat ataaatcttc ttaggataga agaatctaaa
    65161 aaatatctgt tagatagaag catgagtatt agtgatattt gtaagaaagt aggttttaat
    65221 aacagtagtt atttttcaca aatatttaag aagtttaata gtataactcc aaatgaatat
    65281 agaaagaata tgctggataa taagtattaa ataagattat ataatatata ttgtaagaaa
    65341 aattgaaaga gaatgatgga ggaattttat gttttcagtt atttttttat gtataggggg
    65401 gtttttagct gcttttgtag attctatagc aggaggagga ggacttataa gcatgcctgt
    65461 tcttatggca attggagtgc cagtacatct tgccattggt acaaataaat ttgcagcatc
    65521 agcaggatgt ataagtagtg cctatagata tgcaaagtct ggaaaaatta ataacaacct
    65581 tttaaaaaaa ttagtaccat ttactattat tggtagtgtt ctaggtgtga gatgtgtatt
    65641 atcaataagt gaagagatat taaatgtttt ggttgtggtt atgattttaa tagtggctat
    65701 ttacaccttt atttctaaaa atttaggtca agaagataat tttgaagctg ttaataagaa
    65761 aaatttaaga ctagggatgc ttatggctct tataatgggt ttttatgatg gtttttttgg
    65821 tccaggtaca ggaacttttt tgacttttgg atttataaaa atatatggat atgatttttt
    65881 acatgcttct gcaaatacaa aaatattaaa cttaacaagt aatataactt cattgttatt
    65941 attcatgata aatggtcaag tagattataa aattgctata gtgtttgcat tagtgatgat
    66001 aatgggagct tatgtaggtg ctaaagttgc tataaagaaa ggttcaaaga tgataaaacc
    66061 aatattttta gtaatggcat tatttatggt cgtaaaattg gtatatcaaa cattagttta
    66121 gcttatagac tttttaaatt tataagtaat tgtaaagctt ttatatttaa tctaatcata
    66181 tagctttata aaaattctaa ttatcttcta atatagttca tatataatac acatataaac
    66241 tgtaatctta caatagaagg attatatatc ataaaaatat aaccttgatt tatatatatg
    66301 aaatatatgg taatttgtag gaggaatgtt tatgaacata tcaagaagag ctatgaagat
    66361 aattgaactg gcacaaaaaa ttgctaataa aagaggtgta acagtacaag acgcttggaa
    66421 tgatgcgatg aaagaatata aagaaaaata tgaatatgtt gcataagata tgcgttatat
    66481 tttaaatata agaaaatata aaacaaaaaa gattgtctca aaataagatt gtactgagac
    66541 tcaaaagtta gattttatac aggaatggaa ttttcattcc tgtattttta attttacata
    66601 actttaaatt catattcaaa tctatattta acaaggttat gctaaattaa ttttttttat
    66661 tcttttgtta ttataataat aaatatattc tgttatagtt gtatctaatt agtagatgtt
    66721 tttatgtact tttatacagt atatttctcg tttcattctt ttctatttaa ataggactta
    66781 actacttttt aaattgggtt tagctacttt taatttaaaa ttaaaagtat tgttatatat
    66841 ataagtacct caatcattag gtttttgctt taacaattat ggtgcaatac acttttgtta
    66901 agttaaaaaa attagataaa gtaagattaa ataaaagcga cttaagtaaa ttgagaatct
    66961 taatataaaa attttcaata aatatttaga aataactatt tagaaaaatt tctaaatagc
    67021 tattgaaata aaatggatgt tatgttatat tctatttaga tgaaattcta aatagcgagg
    67081 tgatggtgtt ggggtttcca gaaacattca aagctctttc agaccctata agaagagaaa
    67141 tattggttat gttgagggag aataaaatgt cagcaggaga aattagtgaa aaatttaata
    67201 tgacgaatgc tacaatttct tatcatctat ctcaactaaa aaaggcagga ctcatatttg
    67261 aaacaaagta taagaacttt attttctacg aaataaatat ttcagttttc gaagaggtca
    67321 tgctttggtt ttcacaattt atggagaagg gagaaaaaga taatgaaaaa agcgataaat
    67381 aaacaattta ttttatctac tttaatatgt tttataccat ttattgtatc aatatatttt
    67441 tataatagat taccaaatga agttgcaatt cattttgata attatggaaa tccagataat
    67501 tatgctccaa aggttatagc agcttttggt gtaccattgt taatgctatg tatccatttg
    67561 tatacatggt ttagacttga gaatgagcct aagaaagcta atttttcaaa tgttatacaa
    67621 aatttatcaa aatggggagt tgctatactt tcagtaatta tacaaataat gatggtttta
    67681 tattcaacag gagttagttt ggagtctaat ttttatacag gattaatttt aggaataata
    67741 gttattttat ttggaaacta tcttcctaaa tgtaaacgaa attactcagt aggaataatt
    67801 ttaccatgga ctcttaatga tgaagataat tggaataaga cacaccattt agctggatgg
    67861 atttggctta ttggtggaat attattaatt attaatgcat ttattaatat tcctttttat
    67921 aatatctttg taatctttgt aattgtaata ctaccattta tatattctta tttgatgtat
    67981 aaaatttcag gtaaaaataa ctaatataat taatcactat aataatgttt ggataagaat
    68041 aatatatttc agaactgaga tagataataa agaaattact aagatttcta ttttagttct
    68101 gaaaaaatct tacttatatg ataacttatt gagatttcaa aaaataatat aaattaggct
    68161 aatgaacata aaatgaaaat ttgtatttaa tattgaaaat ataagtacgg tgtgatattc
    68221 ttataagaaa aacataggag gtagaagatg aatactagag aaatgaaaga atatagggta
    68281 ttttacaata aaaaattaag cgaaacagat gagtttttta gagtatttgg aagtcttgat
    68341 gataagactt attcagaagg ggctataagt aaaaaaaata aggaattaat gggactagct
    68401 atttcaattt taagccgatg tgatgagtgt atttgttatc atattgaggg ttgtattaat
    68461 tctggagcaa gtaaagatga aattatagaa gctataaaaa taggtgtaat tggtggcggg
    68521 tctattacat atccaaacgc aagatttgca ataaaaattt tagaagaatt taatatataa
    68581 ttttgataga cgtagtttat ttagactaat aataatagtc tatttaagtc tttgaaatta
    68641 agctttattt tctaagttca cttcttatta agtttttaga attgcatata taaatataaa
    68701 tttttaaata atatagcagt tttaaatta
//
LOCUS       DAMJYC010000010        59512 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJYC010000010 DAMJYC010000000
VERSION     DAMJYC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.010"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            224..469
                     /locus_tag="PC472_000714"
     CDS             224..469
                     /locus_tag="PC472_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2726065.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            504..1229
                     /locus_tag="PC472_000715"
     CDS             504..1229
                     /locus_tag="PC472_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2726066.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1251..2564
                     /locus_tag="PC472_000716"
     CDS             1251..2564
                     /locus_tag="PC472_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2726067.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            2770..3309
                     /locus_tag="PC472_000717"
     CDS             2770..3309
                     /locus_tag="PC472_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726068.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3398..5383
                     /locus_tag="PC472_000718"
     CDS             3398..5383
                     /locus_tag="PC472_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2726069.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5607..6575
                     /gene="spoIIIAA"
                     /locus_tag="PC472_000719"
     CDS             5607..6575
                     /gene="spoIIIAA"
                     /locus_tag="PC472_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2726070.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6565..7086
                     /locus_tag="PC472_000720"
     CDS             6565..7086
                     /locus_tag="PC472_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2726071.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7103..7300
                     /gene="spoIIIAC"
                     /locus_tag="PC472_000721"
     CDS             7103..7300
                     /gene="spoIIIAC"
                     /locus_tag="PC472_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2726072.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7316..7702
                     /gene="spoIIIAD"
                     /locus_tag="PC472_000722"
     CDS             7316..7702
                     /gene="spoIIIAD"
                     /locus_tag="PC472_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2726073.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7787..8992
                     /gene="spoIIIAE"
                     /locus_tag="PC472_000723"
     CDS             7787..8992
                     /gene="spoIIIAE"
                     /locus_tag="PC472_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2726074.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9052..9669
                     /locus_tag="PC472_000724"
     CDS             9052..9669
                     /locus_tag="PC472_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2726075.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9681..10235
                     /locus_tag="PC472_000725"
     CDS             9681..10235
                     /locus_tag="PC472_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2726076.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10262..10951
                     /locus_tag="PC472_000726"
     CDS             10262..10951
                     /locus_tag="PC472_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2726077.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNNDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11061..11423
                     /locus_tag="PC472_000727"
     CDS             11061..11423
                     /locus_tag="PC472_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2726078.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11508..12014
                     /gene="nusB"
                     /locus_tag="PC472_000728"
     CDS             11508..12014
                     /gene="nusB"
                     /locus_tag="PC472_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2726079.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11998..13068
                     /locus_tag="PC472_000729"
     CDS             11998..13068
                     /locus_tag="PC472_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2726080.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13065..14270
                     /locus_tag="PC472_000730"
     CDS             13065..14270
                     /locus_tag="PC472_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2726081.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14272..14487
                     /gene="xseB"
                     /locus_tag="PC472_000731"
     CDS             14272..14487
                     /gene="xseB"
                     /locus_tag="PC472_000731"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2726082.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14490..15377
                     /locus_tag="PC472_000732"
     CDS             14490..15377
                     /locus_tag="PC472_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2726083.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15407..15844
                     /locus_tag="PC472_000733"
     CDS             15407..15844
                     /locus_tag="PC472_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2726084.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15878..17743
                     /locus_tag="PC472_000734"
     CDS             15878..17743
                     /locus_tag="PC472_000734"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2726085.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDEGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17876..18688
                     /locus_tag="PC472_000735"
     CDS             17876..18688
                     /locus_tag="PC472_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2726086.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18708..20387
                     /gene="recN"
                     /locus_tag="PC472_000736"
     CDS             18708..20387
                     /gene="recN"
                     /locus_tag="PC472_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2726087.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20601..21467
                     /locus_tag="PC472_000737"
     CDS             20601..21467
                     /locus_tag="PC472_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726088.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVTVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDGIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21560..22051)
                     /locus_tag="PC472_000738"
     CDS             complement(21560..22051)
                     /locus_tag="PC472_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726089.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22112..22552)
                     /locus_tag="PC472_000739"
     CDS             complement(22112..22552)
                     /locus_tag="PC472_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726090.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22713..23771
                     /locus_tag="PC472_000740"
     CDS             22713..23771
                     /locus_tag="PC472_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2726091.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23913..24737
                     /gene="spo0A"
                     /locus_tag="PC472_000741"
     CDS             23913..24737
                     /gene="spo0A"
                     /locus_tag="PC472_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2726092.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25150..26271
                     /locus_tag="PC472_000742"
     CDS             25150..26271
                     /locus_tag="PC472_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726093.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26289..27164
                     /locus_tag="PC472_000743"
     CDS             26289..27164
                     /locus_tag="PC472_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2726094.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27188..27880
                     /locus_tag="PC472_000744"
     CDS             27188..27880
                     /locus_tag="PC472_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2726095.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27888..28712
                     /locus_tag="PC472_000745"
     CDS             27888..28712
                     /locus_tag="PC472_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2726096.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28717..29784
                     /locus_tag="PC472_000746"
     CDS             28717..29784
                     /locus_tag="PC472_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2726097.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29786..30322
                     /locus_tag="PC472_000747"
     CDS             29786..30322
                     /locus_tag="PC472_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2726098.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30390..30995
                     /locus_tag="PC472_000748"
     CDS             30390..30995
                     /locus_tag="PC472_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2726099.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            31007..31921
                     /locus_tag="PC472_000749"
     CDS             31007..31921
                     /locus_tag="PC472_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2726100.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            31963..33135
                     /locus_tag="PC472_000750"
     CDS             31963..33135
                     /locus_tag="PC472_000750"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2726101.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33182..33994
                     /locus_tag="PC472_000751"
     CDS             33182..33994
                     /locus_tag="PC472_000751"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2726102.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34198..35523
                     /locus_tag="PC472_000752"
     CDS             34198..35523
                     /locus_tag="PC472_000752"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2726103.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            35736..36815
                     /gene="mltG"
                     /locus_tag="PC472_000753"
     CDS             35736..36815
                     /gene="mltG"
                     /locus_tag="PC472_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2726104.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            36987..37661
                     /locus_tag="PC472_000754"
     CDS             36987..37661
                     /locus_tag="PC472_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2726105.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            37648..38895
                     /locus_tag="PC472_000755"
     CDS             37648..38895
                     /locus_tag="PC472_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2726106.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            38967..40631
                     /locus_tag="PC472_000756"
     CDS             38967..40631
                     /locus_tag="PC472_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2726107.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(40728..41369)
                     /gene="sigK"
                     /locus_tag="PC472_000757"
     CDS             complement(40728..41369)
                     /gene="sigK"
                     /locus_tag="PC472_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HDF2726108.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            41936..42298
                     /locus_tag="PC472_000758"
     CDS             41936..42298
                     /locus_tag="PC472_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726109.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            42282..42998
                     /locus_tag="PC472_000759"
     CDS             42282..42998
                     /locus_tag="PC472_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726110.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            43018..43437
                     /locus_tag="PC472_000760"
     CDS             43018..43437
                     /locus_tag="PC472_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726111.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            43427..43951
                     /locus_tag="PC472_000761"
     CDS             43427..43951
                     /locus_tag="PC472_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726112.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            44263..44820
                     /gene="efp"
                     /locus_tag="PC472_000762"
     CDS             44263..44820
                     /gene="efp"
                     /locus_tag="PC472_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2726113.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            44912..45367
                     /locus_tag="PC472_000763"
     CDS             44912..45367
                     /locus_tag="PC472_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2726114.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            45530..46240
                     /locus_tag="PC472_000764"
     CDS             45530..46240
                     /locus_tag="PC472_000764"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2726115.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            46294..47364
                     /locus_tag="PC472_000765"
     CDS             46294..47364
                     /locus_tag="PC472_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2726116.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            47392..50946
                     /gene="smc"
                     /locus_tag="PC472_000766"
     CDS             47392..50946
                     /gene="smc"
                     /locus_tag="PC472_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2726117.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            50973..52253
                     /gene="ftsY"
                     /locus_tag="PC472_000767"
     CDS             50973..52253
                     /gene="ftsY"
                     /locus_tag="PC472_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2726118.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMNSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            52356..52724
                     /locus_tag="PC472_000768"
     CDS             52356..52724
                     /locus_tag="PC472_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2726119.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            52721..54073
                     /gene="ffh"
                     /locus_tag="PC472_000769"
     CDS             52721..54073
                     /gene="ffh"
                     /locus_tag="PC472_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2726120.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            54111..54383
                     /gene="rpsP"
                     /locus_tag="PC472_000770"
     CDS             54111..54383
                     /gene="rpsP"
                     /locus_tag="PC472_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2726121.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            54448..54675
                     /locus_tag="PC472_000771"
     CDS             54448..54675
                     /locus_tag="PC472_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2726122.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            54783..55298
                     /gene="rimM"
                     /locus_tag="PC472_000772"
     CDS             54783..55298
                     /gene="rimM"
                     /locus_tag="PC472_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2726123.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            55307..56002
                     /gene="trmD"
                     /locus_tag="PC472_000773"
     CDS             55307..56002
                     /gene="trmD"
                     /locus_tag="PC472_000773"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2726124.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            56187..56537
                     /gene="rplS"
                     /locus_tag="PC472_000774"
     CDS             56187..56537
                     /gene="rplS"
                     /locus_tag="PC472_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2726125.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            56858..57754
                     /gene="ylqF"
                     /locus_tag="PC472_000775"
     CDS             56858..57754
                     /gene="ylqF"
                     /locus_tag="PC472_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2726126.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            58143..59435
                     /gene="brnQ"
                     /locus_tag="PC472_000776"
     CDS             58143..59435
                     /gene="brnQ"
                     /locus_tag="PC472_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2726127.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        24471 a         5855 c        10947 g        18239 t
ORIGIN      
        1 ataggttttt aaaataggat tagataggta ataggttaag ttagaataac ccattaccta
       61 tttttatttg taagaaaagt gtacatagaa ttagaagtaa aaaatacaac taataaaact
      121 attgaaaata ctagataaat aataaataaa aataattata gattttggca tacatattgc
      181 taattataga gataagacga caaaataatt gggaggttta atgatgaaat acaaaacaac
      241 agggacttgt gctacagaaa ttgaatttga agtaaaagaa aataaagtta caaatgtaaa
      301 ctttataggt ggatgtgatg gaaatttaaa agggctaaaa gtattagttg aaggcatgaa
      361 tatagaagat gtaatacaaa agttaaaagg aatagagtgt aaaacaaagc caacatcttg
      421 cccagaccaa ctgtctctag cattggaaaa ttatatgaat ataaagtaat acataaaaca
      481 atcgattagt taggagttta attatgaaac caataatagg gattttagga aatttaataa
      541 taatggaaaa tggtatgttt ccaggtcttg aaaggtcata tgtaaataat gattatataa
      601 atgcagtgct aaaaggtggc ggtagtccag taataattcc tgtaaataca gataaagaag
      661 ttataaaaaa acagattgaa atggttgatg gagtcttaat atctggggga tgggatatca
      721 atcctcaact gtatggagaa gaaacaagag aagaaacgac atttatatat ccagaagtgg
      781 atgaatttga tttaattgca ataagtatag cattagaact taaaaagcct atattaggtg
      841 tatgtagggg actacaaatt ttaaatgtaa gtttaggagg aactttatat caagataata
      901 atcttataga aggaagttat ataaaacata cacaatcttc taaaagacat gtagctactc
      961 ataaagttga tgttaaagaa ggaagtattt tagagggaat tttaggaaaa caattactta
     1021 caaatagtta tcatcatcaa agtgtcaatc agcttggaaa aggattaaaa gccatagcat
     1081 actcaaaaga tggtataata gaagctattg agaaagagga tgaaaatttt gtagtaggag
     1141 tacagtggca tccagaaatg atggtagatt actgtgataa aatggaaaaa ctatttaaat
     1201 attttataaa tatttgttcg aatttataat ataccttggg gggattgaac atgaacgaaa
     1261 agaacaaaat gggattaata agtattatac ttttaggtat taatgcagta gtaggagctg
     1321 gagtttttct attaccagga gatgcaatga aatcatttgg tgtagcaagt atatttgtat
     1381 atatatttga tatgttgtta gttttatcta tggcattttg ttttgctgag gttgcaggta
     1441 aattcaataa aaatggtgct gcctatgtat atacaaaaga agcttttgga gatttttgtg
     1501 gttttgaagt aggtcttatg aaatgggtaa taggatgtat ttcttgggga gctttaatag
     1561 taggttttcc tacatcatta tcagcagtat gggctccagc aggtgaacca catatacaaa
     1621 aaattataat tgtagctatg atagttgggc ttacaattat aaacttatta ggtgtatctc
     1681 tatcaaaaat tgtacaaaat gttataactg taggtaaact aattccatta atactattta
     1741 taggaatagg tatatttttt ataaaaggag ttaattttac ttcttcaaca atggtacctc
     1801 caggagctgg agcaacagag tttggagcag cagcactttt aatgttttat tcatttacag
     1861 gatttgaatc tatagcagtt gcagcagaag atatggaaaa tccacaaaaa aatataccaa
     1921 tagcaattat aagtgtaata gtaattgcat caataatata tatattaaat caagttgtat
     1981 gtgttggtat actaggagat tcgttatcta gtacatctac accagtggca gatgctgcaa
     2041 gaatatgctt tggaaatatg ggagctggat tggttacctt tggaacattg gtttctgttg
     2101 gtggaatttg tatgtgtggt gcttttgtaa atccaagaag ttgtgtagct ttagctgatg
     2161 ataaaatgtt accgagaata tttgcaagaa aagataaaaa aggtacacca tatgtagcga
     2221 taatagctac tatgttaatc actataccaa tagctctatc tggaagtttt gctgaattag
     2281 cagcaataag tgcagttgct agattcatac aatacatacc aacatcttta tccgtattag
     2341 tgtttagaaa gaaaagacca gaattagtag gtacttttaa aactcctttt ggagcagtga
     2401 ttcctctaat tgctgtatgt gttggaacat ggttgttatt ccaagcatct atgcatcaat
     2461 taattatggg attaggagct ttggcaattg gagtaccatt atactttata atgaagtctt
     2521 ataatagaaa agtctatggt gaaaatttaa agaaaatagt ttaattatgt tataacaatt
     2581 tattgagtta aaattagaca ttggcagaaa attaaataca aataagtatc tacaaggcta
     2641 tattaaaata ttattaattt tttattttaa tatagcctta tattgttatc aataaatgaa
     2701 aaatattgta acaacagaag aacaatagta ttatgtttat agacaaaaaa cttattagga
     2761 ggtaaattta tgccaacaat aacattaaaa aatggtgtag acgtacttat tagagaaggt
     2821 gtaagagaag atgctcaaag cattatagat ttttataatg aagtgggtgg agaaactcat
     2881 tttctgtcat ttggaaagga tgaatataaa atatctttgg aggaacaaga aaatgctata
     2941 gaatcagcaa aagcatctga taattctgtt aaactgattg catttataga tggtgaaata
     3001 gttgggattg caacgattga ttctaatcaa aaagcaaaag gaaagcatgt tggagtatta
     3061 ggaattgttg taaaagaaaa atattgggga attggattgg gtaaaaggct tatgttagac
     3121 cttatagaat ggtgtaaatc aaatggaata accaagaaga taactcttgt cactaatgaa
     3181 gaaaattata atgctatagg attatacaaa aaagttggat ttgaagtaga aagcatacta
     3241 aagaaagaat gttattataa tggtgtttat actgatttaa taggaatgtc acttttattg
     3301 ggaatataat tagtgtacat tttatctcga aaaaagtata ttaagtatgg tataattaaa
     3361 aaagaatata acttaatata attttggagg acgaattatg aaaaatctgt gtaaaatatc
     3421 agatgattat ttaaacagca agcttaagta tttaaagtta ttatcaaaac aatatccaag
     3481 tatatcaaaa gcaagtacag aaataataaa tttagaggct atattaaatc ttccaaaggg
     3541 aacagagcat tttataacag atgtacatgg tgaatatgaa ccatttgtac atgtattaaa
     3601 aaatggttct ggtgtaataa agagaaaaat agaagaattg ttttcaaata caataagaga
     3661 cagcgaaaaa aaaatgttag caactcttgt atattatcct gaacaaaagt tagacctaat
     3721 aattaagcaa gaggaaaata tagatgattt ttatagaata aatatttata gacttataga
     3781 actttgtaaa tatgcttcta gcaaatatac taggtctaaa gttagaaaac tgttaccaga
     3841 aaattttaaa tacataatag aagaactttt gcatgagcat gttaaaagtg aacataaaga
     3901 agagtattat aaaagtatag ttgaaacaat agttgacata ggaatagcta aggagtttat
     3961 aattgctatt tctacagtta tacaaaaatt ggttgtagat agacttcatg taataggaga
     4021 tatttatgat agaggtccaa gaccagatat aattgtcgat aagcttattg aacatcattg
     4081 tgtagatatt caatggggaa atcatgatat attgtggatg ggtgcagcct caggtgaaaa
     4141 aacttgtata gcaaatgcac tgaggatatc agctagatat gcgaatttag acattgtaga
     4201 agacatatat ggaataaatc tattgccttt agccactttt gctatagaaa tgtataaaga
     4261 tgacccttgt aaagaattta ttccaaaggt taatgaccaa agtgtaacaa caacagaaaa
     4321 gtctttaatg gcaaaaatgc acaaagcaat aagtataatt caatttaagc ttgaaggtga
     4381 ggttatcaga cgaagacctg aatttgaaat ggaacataga ctcttgctta atatgataaa
     4441 ctatgatgaa ggtacgatta atttaaaagg taaaacatat aaattaaaag atacttattt
     4501 acctacaata gacaaaaaag acccatataa attaactatg gaagaaagaa atgttattga
     4561 taaattagta tcttcattta gaggtagtga aaaactacaa aaacatgttt catttttgtt
     4621 ttctaaagga agtatatatt taaaggctaa ctcaaattta ctgattcatg gatgtgtacc
     4681 gttaaatgaa gatggaagct ttatgtctat gaatataatg ggaaaagagt ataaaggtaa
     4741 ggctcttatg gataagatgg agtctctagc aagagaagga tttttcttta aagataaggc
     4801 agaagaaaaa ctgtatggca tggatattat gtggtattta tggactggga aatgttcatc
     4861 attatttggt aaagatgata tgactacatt tgaaagatac tttatagctg aaaaagagac
     4921 tcataaagaa aataagaacc catattttaa acttagagaa aatgagatgg cttgtaaaag
     4981 attatttgaa gaatttgatt tagaacttga tgaatctcat ataataaatg gtcatgttcc
     5041 tgtggagagt aaaaatggag agagcccaat aaaagctaat ggtaaaattc tcgttataga
     5101 tggtggtttt tctagggctt accaaaaaac tactggtata gctggataca ctttgatata
     5161 caattctaga actttgcaat tagtatcgca tgaaccattt aattctgctg aagaagccat
     5221 tgcaaatgaa agtgatatct tatcgacaac tgttgtagta gagcataagg caaagagaaa
     5281 aatggtgaga gatactgatg aaggaataaa gatacaagag gaaatagaag atttaaaact
     5341 acttttaatg gcttataaga aagggctaat aaaagaaatg tagatgttat agaggcactt
     5401 catgattgaa gtgtcttttt gttaatactt attaaatata ttataaagat taataatagt
     5461 agtttatatt atacttacta agtgaatcca agataaatgg gttcactttt tttatttata
     5521 aacattatta aaaaaatata gatgttttgg ttatattcct tgtcctttat aaatagaatt
     5581 atataaacaa aggaggagga caagagatga ataaactttc tgatgaaata ataaactctc
     5641 tttctacaac tataagagaa aaggtagaaa aagtctcaaa caataatctg aacatagaag
     5701 aaattaggtt acgctcacaa aaaccattga tactaaatgc aaactctaaa gattattttt
     5761 acaatcaaaa aactatgaca cttgatttaa atcaacaaaa ctcatatgta gtaacaagag
     5821 aagatgtaga gcaaactttt caaataatat gtaaatattc aatacattca tttatggatg
     5881 acataaaaaa aggattcata actttgagag gaggtcatag agtagggtta gttggaaaag
     5941 caatagtaga agatggacaa gtcaaaaata taaagcatat atcatcttta aatataaggg
     6001 tatcaaggga aattattgga tgctcggata agattttaag tcatatcata aagggaaaaa
     6061 atcagattaa taatactttg ataatatcac cacctcaatg tggaaaaaca actttaataa
     6121 gagatatagt aagaaattta agtaatggta atgaagatta tggatttaaa ggtttaaaag
     6181 tggctttagt agatgagcgt aatgagatag caggagctta tctaggtgtt ccacaaatgg
     6241 atgtgggaat aaggactgat ataatagaaa cttgcccaaa agacttgggt attacaatgc
     6301 ttttaaggtc tatgtcacca aatgttattg tgacagatga aattggaagt gaaaaagaaa
     6361 taaaggcatt atacacagca ttaaatggag gtataggatt aataactact gtgcatggag
     6421 attcaataga ggatatacaa aatagaaaag aattaaatag acttctggat aaagagttat
     6481 ttaaaaaggt tataattctt tctgcaaaaa gaggagcagg aacaattgaa aaaatttatg
     6541 atttagaaga aaagagatgg tattttgcaa attaaaataa ttattatagc tttcttgata
     6601 ggaagtagtt atttgatagg tgaacaaata tacaaaactt atactcgtag acataaacaa
     6661 ttaaacgatt taataagagt tttagaaatt ttgaggatgg atttatcttt tggactttat
     6721 acattagaag aaatctttaa taggataggt ggaaataaag agttttgttt ttggaaattt
     6781 ttctatcaaa tatcagaagg acttcacaat gaacaaagta aaactttgga aataataata
     6841 tcagaaaata ttgatgtctt aagtaaagaa acttatctag gcaataaaga aattgaggaa
     6901 ctgaaaaatc taatacttac tttagggaaa agcgatatag agtcacaaca aagaatgata
     6961 gatttatcta tagaaaattt gaaaaaacaa acctatgaga caaaggaaga tataaacaaa
     7021 aaaggcgtat tgtataaaaa attagtgaca ttcataggta taggtatatg tattatattg
     7081 atttaatatg gggaggttta ttatggaaat atcattgata ttaaaggttg caggtgtcgg
     7141 tattctaata tcagtattga atatgatatt ggaaaaaaca gatagaaaag attgggcagg
     7201 tcttactaca ttagcaggtg taataatcgt attgggtatg gtaataacag aaataagtga
     7261 cttgtttaat actgtaagaa caatgtttca actttactaa agaggaggga gtaatttgga
     7321 aataatgcaa ttaataggaa ttgcaatcat ttcaactaca ctctgtttag taattaaaaa
     7381 agatagacca gagatagcaa atttcatagc tataataact ggagtaataa tactattgtc
     7441 agttatgttt aagctaaatt ttatagtaga tagtatacaa gatttggcta acaaggctaa
     7501 catcccgact atgtatatat cattaataat aaaattaatt gggatagcat acttaatgga
     7561 atttgcgata caactttgta aagattgtgg ggaaggaaac attgcttcaa aactagaatt
     7621 tggaggtaaa ataatagtta tgtcaatgtc tttccctata cttttgtcca ttgtagaaat
     7681 ggtggtgaat ataattccat agatgtgtat cttggaatga atataagtat attagagagc
     7741 tttcgttgta ttgaggttta tagagtgaag agaggcatgg ttgcttatga aaaaaagatt
     7801 tttaccaatg attataggtt ttttatttac tttctttttt gtaaatatct ttgctattat
     7861 gatatttgct aatgaagtac caagtagtga agataaagaa tatggtgaaa caaaaaatag
     7921 catagataag tatatagatg gtcaattaga caagctagat ataaatgaga ttcaagacta
     7981 tataaataaa gaaatagtta ttaatgatgt taatttaaaa tcatttgtaa aagatttaat
     8041 tagtggagaa aaaaatatct tagatttatt caataaagat ggattgaaga tattgatgtt
     8101 tgatgaattt aaagcaagtt taaaggtagt agcagtaatt ttggtactgg ctctgttatc
     8161 atctatttta aagagtctgg aaaactcttt ttcatcaggt gcagtaagtc aaatagcaac
     8221 atatataata tttatcacaa tggtatcact tacacttatt ggttttaaag atgtattaca
     8281 gatttgctat gatgcaatag accatactgt aggtcttatg caagttataa tgccaatatt
     8341 aataacattt ttacttttaa ttggattccc aataacgtcg actactttga atcctatctt
     8401 cataggagga gtaacattta taaatgtatt ttttaaaaat tttctatttg tatctattac
     8461 tgttgcattt ggaattttaa taataaacaa tttatcaaaa aatataaggc taaagagatt
     8521 tttctccttt gtaaaacaaa taaattatgt ttctattgga gctatgttta ctgtttactt
     8581 aggtcttgta tccatacaag gactgtatgt aaccagtttt gataaattca gtgtaaagac
     8641 tgctaaattt gctataggta attttattcc tgttgtaggt ggatttgtat ctgactctgt
     8701 ggatatttta ttatcttcat ctcagcttat aaaaaatata tttggaggaa tagggcttat
     8761 cttacttgtt ggaatttgct tacttccagt aataaaaatt ttatcagtaa tagtagtgta
     8821 caaattagca gcaataattg tagagcctgt tggagaggat ggaatatcta attttttaaa
     8881 tgaagtggca aatttaatga taataatgct agcatcagta attgctataa cagtaatgtt
     8941 ctttgtaact gtagctatct tgacctcgat aagtgtcgta agtcaagggt aatttatata
     9001 tcagtgaaaa ataattacat ttaatctaat gtttttagag aggaggatgc tatgttagag
     9061 ggcataaagg catggatagt tagtgttttg ataggagcat ttatagtaaa tatagtagat
     9121 atgattttac caagttctaa gataaaacct tatgtcaacc tagttttaaa ctttatgttt
     9181 gtatttattg taataactcc agtagtagga tttttttcaa aagatatgag tttagaagat
     9241 agaatattaa aatctatggg aaactacaac aagcagtatg tagatagtac aaatgctttg
     9301 gcaaaagaga ctggaaataa tagtctatcc aagggatatg aggatgggtt aaaagaagta
     9361 cttaaattaa aacttgatga atatggttat gatttagaag atatagagct taatggggca
     9421 aatataaata atataaaaat aaaagaaaag aataatagtg caaaaagtaa taacagttcc
     9481 aatgaagaaa ataaaaataa tagtactaaa agagaggaag aaaacataaa ctctaatgac
     9541 aaagaaaatt ctaaacaagt cttcaaaaaa ggtacagagt atgggttgaa cctaaatgaa
     9601 gaaaaattaa aaaacgactt aataaaggtg ctggatgttt ctatagaaga tatacaaatt
     9661 gataaatagg agttgggaga atgttgaaaa acttaaatga caaagataag agaaaggtat
     9721 attcactaat aactatagca ggaatatgtg tggtttcatt agtattatta tcttgttttc
     9781 ctagtagcaa aagcgataaa caagttggaa aaacagagac taataaaaat gctgaaaagc
     9841 aagtcactaa agaacaagaa aaagatgatt tagagtcaaa attaacagca atactatcta
     9901 aaattgatgg tgcaggagac gttgatgtaa tggttacatt tgaatcaagt gaagaaatac
     9961 aacctgcttt taattcaaat aatacaacag aaacaacaga ggaaaaagat gcacaaggtg
    10021 gagaaagaac agttacgact tctagtgaaa ataaaacaat gataacttca aattcaagtg
    10081 accctgtagt tataaaaact acagagccaa agataaaggg agtgatagtg gtagcaagtg
    10141 gagcaagtga cccaagtgtt aaagagacac tttatagtgc agtacaaaca tcattacaag
    10201 tagctggtca tcaagtagaa atatattcta aataaataca tataaccggg gggataaatg
    10261 aatgaagttt aattataagg gaagaggatt tgtaataata actttaactg caatgttagt
    10321 ggtagtagga acagtgaatt atcaactaag taagaaatct ttattagaaa catcaaaaga
    10381 gtttaaggca tatgaagaag cacagttgca aaaaaataat gatgagagtg atagtagtaa
    10441 gacagaggat tctagtaata tagataaaca agatggtaag gaaagtgctg atattgatat
    10501 agttgatagc aaagctagta aagtaaaaga aaaggcaact gagacaagta aggaaataaa
    10561 agctcaatta tcatcagaga aaaatatgaa aaaggcatct tatattttag atatgaaaat
    10621 gaatagagaa aaacaaagaa atgagttagt tcaagattta aatgaaatga taaataatcc
    10681 atctacaaca gaagaatcta gaaaagaagc ttctaatatg aaattaaata tagttaaaat
    10741 tcaagaaaaa gaacttcaaa tagaaaacct tttgagtaca aaagggtatg aagaagcgtt
    10801 agtttatata agcgataaca aagttaatgt agttgtaaat gaagctaaat tagaaaagaa
    10861 agatgcagca aaaatatttg atttagttgc agaacaagcc aatgtgaagt atgaaaatat
    10921 taaacttaca aataataata gtaataagta aagtataaac ctcagaatta actgaggtta
    10981 ttttcttgca aagtagactt gtatttggta ttattatact tataaaaata gttccttaaa
    11041 atcaggaggt aagttttaat atggaagata ataaatttgg acaagtaaaa atatctaatg
    11101 atgtaatagc tacaatagct ggtttagcag cattagaagt tgaaggcata gaaactacag
    11161 caacattaac agataagttg ttaaaaaata atggagtaaa aatacaaatc gaagaagagg
    11221 atgttaattt agatgtaatg gttacgataa agtatggaat gtcaatacca gatacagctt
    11281 ttaaagttca agaaaatgta aaaaatactg ttgagacaat gacaggactg aaggtttctc
    11341 aagtaaatat acacattcaa ggaatcagct ttaagaagga taaagttgat aaagaagaag
    11401 caaaagcaac taagaaaaat taatattagc cctctgaaaa gtcagagggt tttttgctgg
    11461 taattgattt tatatggata attaaactta aaggagaatt ttgattgatg aaaaaagata
    11521 gggcacaaaa gagtacaaca agagaatata taatgaaact tatataccaa ataaatatta
    11581 ataaagaaga ttttgaaact ttagaagata aagtagataa ttttttaaaa gataattcag
    11641 aacatattat aaatagatat aaagaacttg cacttcaata ctcaaaaaat acaaatttaa
    11701 agttagaaga tactgaaatt gaagatgtta tagacaaaaa atatataaat acagtatgca
    11761 aagcattgaa agaaaatcat gacaaaatag atgagttaat aaataagcat gctaaaaact
    11821 ggacagtaga tagaatgcca aaggtagatg tttctatact tagattgtct gtgtgtgaaa
    11881 tactttattt agacactcct aataaagtat caataaatga agcagtagaa cttgcaaaaa
    11941 tatattgtga tgataagtct cctaaattta taaatggtat attgggaagt gttgttgatg
    12001 aaattgggaa ataatataat aattggaatt gatactagct gctatactac ttctatagca
    12061 gctatctctt tagataagaa agttatattc aatgaaaaaa taatgttaga agtaagggat
    12121 aattcaaaag gtttaaggca aagtgaagct gtatttcaac atatcaataa tttagggatt
    12181 cttagtgata ggataaaatc ttttaaagat aagtttaatg tagaaggagt ttgctcatca
    12241 aaaaaaccga gacctgttga aaactcatat atgcctgtat tcaatgtagg tcataatttt
    12301 ggaaaattat tgtctagtat atatggttgt aggttttatg agactactca tcaagagaat
    12361 catattgaag caagtttatt aaatagtaaa ttaaaaaata ataataaatt tatttctgtt
    12421 catatgtctg gtggtactac agagatactt ttaacaagca aacaagatag tcatcacaat
    12481 gtatgtgata ctaatttagg taaaattgcc aaaataagta ttaaaaaaga tgataaaagt
    12541 aagttataca ataattttgg atataatata gatataattg gaggaagtaa agatattagc
    12601 tttggtcaac ttatagatag agtaggaatt aagcttggat ataaatttcc ttcaggaaag
    12661 tatttggatg aaaatgcttt aaattgtaat cttaaaatag aaagtggtct gaaaacttct
    12721 gtaagagatg ggtatatgaa tttatcagga ttagaaaatc aggtaaataa gattataaat
    12781 gataatggat ataatactaa tcaaaaagaa tatatatcta aattggtatt agattcagta
    12841 gttagaaata tgtttaaatc tttagtgtat ttatgtgaaa cttataatgt aaatgaagta
    12901 gtatttgctg gtggagtttc tgcaagtaag tatattctta gagaattaag cgtgaagtta
    12961 agaaaaaagc atatagaagc ttattttaca gaaccacaat attcaactga taatgctgtc
    13021 gggtgtgcta taataggatt gaataacttt ttaggagaaa gagtatgaaa ctaagagctt
    13081 tagatataag tgaagcaaac tcatatatta aaagaatttt aataaacgac ccaatacttt
    13141 ctaatcttaa agttaaagga gaaatttcta attttaaggt tcatagtagt gggaatgtat
    13201 atttatcact taaagatgaa acatcaaagt taaactgtgt aatttttaaa agtaatttta
    13261 atcgcaattt aaaattagat aatggtgtaa aaattatagc taatggatat atatcagtct
    13321 atgaaagaga tggtgcgtat caattataca ttaatgaaat tgaaatagag ggaataggaa
    13381 atttacacat agagtttaat agattaaagg aaaaacttaa taaagaagga ttatttgacc
    13441 ctaaatacaa gatacctata cctaaaatgc caaactcaat tggcgttata acatctccaa
    13501 caggtgctgt tataagagat ataataaatg taataaaaag aagatatccg aaagttaata
    13561 taaagcttta tccagtaacg gttcaaggag ataaatcagc tgaagaaatc tgtgaagcaa
    13621 ttagattttt caatcatatg aagaatgttg atacacttat agttggtaga ggtgggggtt
    13681 cgatagaaga actttggtct tttaatgatg aaatggtagc aagagaggtt tttaattctc
    13741 aaattccgat aatatcagca gttgggcatg aaacggattt tactatttgt gactttgtgt
    13801 ctgatatgag agccccaacg ccatcagcag cagctgagat tgctactcca tcattggatg
    13861 atataaacta taaacttgga aacataaaaa gtaggatgag taagtctctt acaaatcaaa
    13921 tagagctaga ccaatatagg ttagaaacag tatttaataa gataaataac tatttagatt
    13981 cgtacacaat aaaagataaa gttatacaat tagacaaaat atatgataaa ataatttttg
    14041 gaatagaaaa taatttaaaa ttagaagatg aaaaattagt aaaaattgga gcattactcc
    14101 ataatttaag tccattagct actatggata gaggttatag tataactcaa aaaaatggaa
    14161 aagtaataaa tagtataaaa ggattaaaaa taaaggatag tatagatata gtattaaaag
    14221 atggaaattt agaatgtatg atagataaaa ttgaaaataa agagggatag tatgaattta
    14281 acctacgaag aagcttataa aagattagaa agtattttaa gtgaattaga atctaaaaat
    14341 gctagcttgg atgaatcttt aagcctatat gaagaaggta taagccttta taaacattgt
    14401 aataagcttt tagatgatgc taaattgaaa ataagtaaat ttaatcaatt gggaatagaa
    14461 gaagacttta aaatagagga ggaataaaat tggaatttaa acaatgtcta aaagaaaaag
    14521 caagttttgt agaaaaagta cttaaagaat atatgcctaa agaagaaggg tatcaaaaaa
    14581 ctgttattga agctatgaac tatagcttaa gtgctggagg taaaagatta agaccaatac
    14641 ttacattgga ggcttgtaaa atagttggag gtaatgaaga tgaagctatt ccttttgcta
    14701 ttgcaattga aatgatacat acatattcac taatacatga tgatttgcca gctcttgaca
    14761 atgatgattt aagaagagga agaccaacaa atcataaagt atatggagaa gctatgggga
    14821 ttttagcagg agatgctttg ttaaactatg cttttgaagt aatgcttgca ggctcaatca
    14881 ataaagaaaa tccagaaaag tatctaaaag ctataaatga aattgccaaa ggtgctggta
    14941 tatatggaat gataggtggt caagtagtag atgtagaaag cgaaaataaa caaattgaaa
    15001 aagaaaaatt agattatata catatgaata aaactgctgc aatgatggta ggctgtatga
    15061 gagcaggagc tactataggt ggagcaaatt ctgaacaaat ggaagaaata actaaatatg
    15121 caaaaaatat cggattatca ttccagatag tagatgatat actagacata gtaggagatg
    15181 aagctaagtt aggtaaaaag gttggaagtg atatagaaaa tcataagtca acatacccat
    15241 ctcttttagg tttagataaa tctaaagaaa ttgcacataa tttgattgat gaagcaaaga
    15301 aaagtataga aaaattatct gatgatgtag attttttaaa gggattggct gaatatataa
    15361 tagatagaga atactaaaat atacagctaa aatcgaggag ggaaaaatgg attttttttc
    15421 ggagattttt aacaatgggg ctttgggaat aagtctgatt gcttgtttct tagcacaatt
    15481 tataaaaata tttactggta aggaaaaaag aattgagtta tcaagaatac ttatttcagg
    15541 aggtatgcct agttcccata gttcttttgt tacaagttta gctacagtgg taggtattga
    15601 aaaagggttt aattctactg attttgctat aattaccgtg cttgccttaa ttataatgta
    15661 tgatgcagca ggtgttagac gggctgttgg taaacaagct actatattaa atcaaatggt
    15721 ggcagatata cagcatggaa agcatataga gcagaagaaa ttaaaagaat taataggtca
    15781 tacaccacta gaagtttggt ttggagcttt acttggaata gtaacagctt taatattgat
    15841 gtaaattgaa aatttacaga ataaaggtga ggataatatg tataaatatt tagataaagt
    15901 aaattctcca aaggatataa aaaatatgag tatagaagaa atggatttac ttgcaaagga
    15961 tataagaaag ttcttagtaa aatctgtttc aaagacagga ggacacttag cttctaatct
    16021 aggagtggtt gagttaacat tagcacttca taaagtattt gatagcccta aagataagat
    16081 tgtatgggat gtagggcatc aatcttatgt tcataaaata gttactggaa gaaaagattg
    16141 ttttgtatct ttgagacagt ttaatggact aagtggtttt ccaaaggaga atgaaagtcc
    16201 tcatgatatt tttgatacag gacatagcag tacatctatt tctatagcta caggtattgc
    16261 ctgtgctagg gatataaaaa aagaaaacta tagtgtgata tctgtgatag gagatggttc
    16321 tataacagga ggtatggcac ttgaagcttt aaatcaatta ggatatatag ataccaatat
    16381 gatagtaatc cttaatgata atgagatgtc catagataaa aatgttggag gaatgtctaa
    16441 atatctgtca agtattataa gaaactcaac agttgagaaa atgacagatg aagttgataa
    16501 aattttaaat gttacacaaa caggtgaaat tttatctaag actgcacata gatttaaaga
    16561 taaactaatg tatagctttt ctcctcagga ctgttcattt tttgattcat taggtataag
    16621 atattatgga cctatagatg gccataatac taaagaacta atagatatat tgagaaaagc
    16681 taaacacaaa aaagggcctg ttcttttaca tgtaattacg aaaaaaggaa aagggtacag
    16741 atttgcagaa gaacaacctg ataaatatca tggagtatca aagtttgata taaagacagg
    16801 tgttacatct gcaaaagtta agtctatgtc aattagtgtt ggagaaaaac ttgttgatat
    16861 ggctaatagt aatgaaaata tagtagcaat aacagctgca atgccatctg gtacagggct
    16921 aaacctgttt gaaagtgcat atccaaaaag atattatgat gttggtatag ctgaacaaca
    16981 tgcaacagga tttgcagcag gtcttgcaaa aaatggtatg aaaccttact ttgctgtgta
    17041 ctcatcattt ttgcaaaggg catatgacca agttattcat gatgtatgca taacaaaaaa
    17101 gccagttact tttcttatag atagagctgg attagttgga aatgatggag aaactcatca
    17161 tggtatgttt gatttaagtt acttaaattc tattccaaat attgtagtaa tggctccaaa
    17221 agacacaaga gagatggagc ttatgatgga tttgtcatta aaattagatt gtccactagc
    17281 aattagatac ccaagaggaa gtagttatta cctagataag ggagagtatg gagagattgt
    17341 actgggtaaa tatgaggtat tagatgaggg tcaagataca gtcatacttt gtattggaag
    17401 tatggtaaaa catgctttag aggctaaaga aatattatca agagaaggaa taaatccgac
    17461 aatagttaat gctagattct taaagccaat tgatgaaggt atgttaaaag cattattaaa
    17521 aaatcataaa aatgtagtta ctatagagga taatattgta actggaggat ttggaagtag
    17581 aataaataag tttattatag ataatgaata taacgtaaat atacttaata tagcaatacc
    17641 agaagaattt gtaaaacatg gcaatataga tgaattatat gactttgtag gattatcgcc
    17701 aaaaagtatt gctgataaga taaggaaatt agttattgag taaaaatcta atgtagatat
    17761 tgtaaattaa aatttatata aaatatagaa tttatcaatt agaataaata tcaagtgtgt
    17821 tacttcaagc tactttagtt atcattaaag tagtttttaa cagaaaggta attgtatgaa
    17881 aaaaagaata gatttattat tagtagaaca agggtacttt gaaagtagag aaagagccaa
    17941 aaaagcaata atggctggat tggtttttgt tgataatcaa aggtgtgata aggctggtac
    18001 agaagtaaaa gaagattgta gtatagaagt aaaaggaaat ccaataccat atgtcagtag
    18061 aggtggttta aaacttgaaa aagcaatgaa aaactttgat ttgactattg atggaaaggt
    18121 atgtatggat ataggggcat ccacaggtgg ttttacagat tgtatgctca aaaatggagc
    18181 aataaaagta ttttctatag atgttggtta tggtcaactt gcatggaaat tgagacaaga
    18241 tgatagggtt gtgtgtatgg aaagaaccaa tataagaaat gtgacaatag aagatacaaa
    18301 gcagtttgct gattttgcgt caattgatgt gtcttttata tcgttaaagt tagtacttcc
    18361 aaaggcaaag gagttagtac gtcatgatgg ggaagttgtc gctttaatta aacctcaatt
    18421 tgaagcagga agagaaaaag taggtaaaaa aggagtagtt agagaaaaat ctacacatat
    18481 agaagttata aaaatgattt ctgatttctc agtcgaaaat ggatttgaga ttttagggtt
    18541 agatttttct cctattaaag gtcctgaagg taatatagaa tatctaattc atttgagaaa
    18601 tggaaatgaa ggatatgaat ttgatggtga gacatacaat aacaaaatag tagaagttgt
    18661 tgaagcttct cataatttag acaaataggt taaaaggggg cttagatttg atcctcgaat
    18721 tatatatgaa aaattgtgcc ttagttgaag aactaaggtt gaatattgat aaaaacctaa
    18781 atatacttac aggtgaaact ggttcaggaa aatctataat aatagatgca cttggacttt
    18841 gtttaggtga taaatatgat aggtcttttt taagaaaagg aacagataag ggacttgttg
    18901 aggctgtatt tttttcagac aatagatatc ttaaaaaaat attagaagaa aatgatataa
    18961 gtatggaaga tgataattta ttggtaataa ctaggttgat atactcagat ggtaagagta
    19021 ctgctagagt aaatggaagg actgttaaag tgtctcttct taaagaaatt gcatctacac
    19081 ttatagatat acatggtcaa catcaaaatc aagctttgtt taataaagat actcatttaa
    19141 aatttctaga cctttttgga gaaaatgaat tggaagagtt taaaattgct tataaaaatg
    19201 tgtaccataa gtatagtgaa gtaaaaaaag ctttaaattg cttgacagaa aataaagatg
    19261 agatgcaaat tcaacgagaa atagatttat taagatttca aataaatgaa attgaagctg
    19321 caaatctaaa taagaatgaa tatgaagatt tattaaaaca aagagaagtt tatagaaata
    19381 gtgaaaagat atataataat ctaaattcga gttatagcaa gttacataat ggtgagtata
    19441 atgttattga cctaataggt cttgcatcta aggaacttaa tgacatatct aaatatgaca
    19501 gtgttttaag tgaatatagt gatactgtag aaagaatcat gtatgagtta caagacatat
    19561 ctggtgagat aagaaattac aaagataata tagactttga accttatgag ctagagcaga
    19621 ttgagcttag aatcgacgag attaataact taagaagaaa atatggagac agtatagatg
    19681 atatatttga gtattatgga aaaattaaag atagattaga tgaaatatta aacagagatg
    19741 aaagagttga acagttaaga agcaaactga tgaatataga agaagattta aaaataaaag
    19801 ccagtaaatt gactaaagca agaatagaag tagcaactac tttagaaaaa atacttcttg
    19861 atgaattaaa aagcttaaat atgaaaaatg taatgttcaa ggttaatttt gaaaaaggtc
    19921 catttacttt agatggagta gatgatattg aatttatgat atcttttaac ttaggagaag
    19981 atattaagcc tatatataaa gttgcatcag gtggtgagat gtccagattt atgttagcgt
    20041 ttaaaaccat attggctgat atagatgata ttgatacatt agtgtttgac gagatagata
    20101 ctggaataag tgggatagca gcacagatag ttggtgagaa attgagtgat atagcaaaga
    20161 aaaaacagat aatatgtata actcatttac ctcaaattgc tgcaaatgca gacactcatt
    20221 attgtataga gaaagatact tcaaataata ggacgtttac caatgttagt aagttaaatg
    20281 aatctcaacg aaaaaatgaa attgccagac ttatagcagg taacaatata acagaaaaaa
    20341 ctattgaaca tgcaagtgaa attattgagt tggctaaaaa atgttagtga aaaacaagca
    20401 ttttaatagg tgtaaagaat ttttatagaa atgctttaca tctatttttt atgatgaaat
    20461 tattttaaaa tatctagtgc ctatattgaa gaaaagtaga tttaaaaata gaagaaaatt
    20521 tgtataaatt tgtaactttg aaatttctaa atgcttaagt ataatattat ttattaaggt
    20581 agtagtgagg gggacataaa atgaataaaa aagatactat gcacttaatt tttacaattg
    20641 taatttttat tttattatgg tatattgtgg taacggtaag tgagattaat aaaaatattg
    20701 aaagttgtag taataaatta gattatttgt cacaagagat tatgagtact aaatcggata
    20761 ttagtaatac tataaatgaa gaaatgtcaa aaagttatat aacaaagagt attgacttaa
    20821 aagtaaaaaa aatcgagaaa aaagagtgtg ttattgatgt agatgttcaa ttaagtaaac
    20881 ttggaaaaaa tggaaaagta ttttttatgt ataaagaaga tagtgataaa tggaatgaaa
    20941 ctgagatgac aaaacatgga gaattatcat atgcatgtga aataaagata aatacaggaa
    21001 gtgaatatga ctataaagta gttacaagtg gagctatatc tgaaagttct gatgtgcaaa
    21061 aattgactaa atctgattat atgcctgaac aacccatatt taattctggg ataagagata
    21121 atagagaata ctttattgaa atagtagaaa atagtaatct tttcaatcat gagagtgaaa
    21181 agtcaaaaaa taaagtttat gataatatta gattagaaaa agtagatatt attgtaaatg
    21241 aaggaaaaaa agacactata tacaaagcca aattagctga aggcttagat gatggcaaaa
    21301 ctaataattc aaatagaaat caagtgaatt atgaagtaag ttttcctaaa agggatattg
    21361 atggcataat ttatattaaa gctaaactta catacaataa tggagtagaa tatacaaagg
    21421 atataactga agatataact atgggattac aagatttaga aaaataaata catatttaaa
    21481 taaatattca aatagatttg ttcaataaaa tagagtataa ataaaactgt agtagtttta
    21541 cttatactct attaaaaaat tataatttta attccatata tctagcccct tcaattggat
    21601 tataagtata aggttctata tcataaaaac caaacttttc atataatcct tgtgcacttt
    21661 tcatgcttgg aagagtatct aatctcatgt gtgtatatcc aaatttttta gcttcttcaa
    21721 tgatttcttc caatagtatt tttcctattt ttaatcctct aaattgattt cttacatata
    21781 atctttttaa ttcacatatt ttaccttcaa gtttttttaa agcaacacaa cctgccaaat
    21841 tttcatcaac aaatgctaat atcaatgagc ctgaaggttt tttatatttt cctggaagtg
    21901 tttttaattc attattaaag tcttgaaaac ataagtctat atttagagaa tttgaatatt
    21961 cagtaaatag aagttttacg ttttcaaggt tttctaaatc tttttcttca acaaattgta
    22021 aagttatatt ttgagccatt ttatccgcca ttatataaac atgtttatat aatgacttca
    22081 cctccttgat atatgtatat ttttcttaat atcaattaga ttttaatacc attacttttt
    22141 caccaccata gtcaagctct ccattaaact caaatccaaa atctttataa agcttaatag
    22201 ccattttatt gtcttcgaat atactaagat atagttcatc acagttatat tcatttttaa
    22261 gatgatttac aagaaaatct aatgatgctt ttccatatcc tttaccttga tattttgaat
    22321 caatcataaa tctatcaagc caaactctat catcttctaa acgaccatac atagcaaatc
    22381 ctattaaaga attttcatca tatattccaa caggtaccca agctgttata aatttagatt
    22441 cagcaataga aagtgcattg ctttctatat aatctaactg acttttatct acagacaaac
    22501 taactaaatc atgccaatta tttatgtctg caattcttac atttaatttc aagatattac
    22561 cgcccttcaa actttttgtt acaatctaat actatcatat tatgtattaa aaatcactta
    22621 ttaagatttt aaataaactt tttaaatgaa taaattctca ggaaaaagga attataaata
    22681 cataataata cagcatagga gtagatggat atatgaacaa aataaataaa aatttcaaaa
    22741 atatttggat aatattagga gctgtatcga tttttaccat ggttataatt tttaataaaa
    22801 attgtaatgt taataaagag actcaaatag aagcttttac aattaagaat catgataaaa
    22861 aatttgtata tcctatggga aatataattg gagtaaaagc aaatacagat ggtgtattgg
    22921 tcttaggata tgaggaagag gatgtagact atattggagg catacaaatc ggtgataata
    22981 tagtaaaaat agataataaa aggataaaaa atagtcaaga tgtatcagag attttgaata
    23041 agattaaaaa aagtaaagtt gaagtgactt ttgagagaaa aaataagtat aaaacagaag
    23101 taatagagac taaaaaagaa aatggcaagt ataaacttgg attatgggta agagataaaa
    23161 tatctggaat tggaactatg actttttacg accctagtat ggaaaagttc aaagcaatcg
    23221 gtcatgccat aaaagattcg gatactaatg aactattaaa gataaagcaa ggatatatat
    23281 ataagccaga acaattaaaa atagtaaaag ctagtaatga aaaagtaggt aaaataaaag
    23341 gagattttaa tgacagcaat ttaatgggta atttctcaaa taattcagag ttaggcataa
    23401 gtggtaatat tacagaaaac cataataaag agtttaatgt agcaaataaa gaaaagcaat
    23461 taatagaagt tggaagaccg caagatgtaa aaataggaga tgcagtaatt ctttttgagg
    23521 ataaaaacaa aaatataaca agctatgata taaaaataga aagtatagta tatgataaag
    23581 gaaattatag agatatggta ataaaagtag tagatgacaa gttattggaa tacacaggag
    23641 gtatcgtaca ggggatgagt ggagctccaa taatacaaaa taataaaatt attggtgcaa
    23701 taactcatgt ttttagagat aatccgaaaa aaggatatgg tatttttata gatgaaatga
    23761 taaaattgta ggtgaggcat taaaaaattt tattatttta tcaattatct aggaggaata
    23821 taattttgga gtgtcgaata tgctttagag tagataatta ggaagcaatt gtgtaaaaag
    23881 tttagttttc tgtaataaga agatgttttt taatgggggg atttttagtg gaaaaaatca
    23941 aaatagtttt agcagatgac aataaggatt tttgtcaggt attaaaagag tatttgtcta
    24001 atgaagatga tatcgatata ttaggcatag ctaaggatgg aattgaagca ttagacttag
    24061 taaaaaagac acaaccggac ttattaatac tagatgtaat aatgccacat ctagatggat
    24121 taggtgtaat tgaaaaattg aatactatgg atatacctaa aatgccaaaa ataatagtac
    24181 tatcagcagt aggtcaagat aagataactc aaagcgcaat aaatctagga gcagattact
    24241 acatagtaaa gccatttgat tttgttgtgt ttataaacag aattagagag ctagtttcta
    24301 atagagttac acaagttgag ccaaaaccta gaccagttca agagactcaa atgactagaa
    24361 gtgattttgt taagaatgta ggaaatatag agacagaaat cacaaatata atacatgaaa
    24421 taggagtacc agctcatata aaaggatatt tatatttaag agaagcaata aaaatggtta
    24481 ttgataatgt tgagctttta ggtgcagtaa caaaagagtt atatccaagt atagctaaaa
    24541 aattcaatac aacaccaagt agagttgaaa gagcaataag acatgcaata gaggttgcat
    24601 ggagtagagg aaaagttgac acaataaatc aattatttgg atatacggta cacaatacta
    24661 aaggaaaacc aactaattca gaatttatag caatgattgc tgataaatta agactagaac
    24721 atagtatggt taaataaaca agacataaaa agtaaggctt tttaattaag gcattggcta
    24781 taaatgcgta ttacaagcag cgaaacggta taaccactag ggttataccg tttcgctatt
    24841 ttaataaata taaaaatttt ctttattatt tgcttactat atcaatataa taactttatt
    24901 atactatgga tatagtatgt gtctttacaa gttgtaaact gacagtggtt tattttttaa
    24961 tataaatatt gactttgatg caggtaaact ttgtattttt aagcgtatta tggaatatgt
    25021 taaataaaaa aatgatgaaa tatagtattg taaatgccaa agatgcaaaa caaacttaaa
    25081 acatttattt tattgttaag taatgctata atataatgtg attttaataa tgatagtgga
    25141 ggtttaaata tgagagtcga ggcccctata aaagtagatc gaaaaaccaa aaaacttgct
    25201 aaaagagttg aaagtgggga aatagcagtt ataaatcata tagacataga tgaagttgct
    25261 gcaaactctt tagtagaagc taaaataaaa cttgtcataa atgcggctcc ttctataagt
    25321 ggtaggtatc ccaataaagg tccagggata ttaactgaaa ataatatact aataattgat
    25381 aatgttggag aagagttatt tgaagaatta aaagaaggag aaactataga agttgtagat
    25441 ggaaagatat atagaaaagg aaaattttta ggtgcaggag aagttttaga caagtatgag
    25501 gtttcacacc aaataaaggc tgcctatgag aatttagctg tagaattgga tagatttata
    25561 gataatacta tagattatgc taaaaaagaa aaaggattta tacttggaga agtagaaatt
    25621 ccaaaagtga aaactaatta tgctaataaa catgtattga tagttgtaag aggtcaggac
    25681 tacaaagaag atttaagtac tatgctttct tatatagaag aggtaaagcc agtattggtt
    25741 ggtgttgatg gaggagcaga cgctttaatt gagtttggat atactccaga tgtgattgta
    25801 ggtgatatgg atagtgtaag tgatgaagct ttaaaaaagg ctagtgaaat agtagtacat
    25861 gcttatacag atggaagagc accaggtctt aaaagggttg aggaattagg cttagatgca
    25921 gttgtatttc ctgcaccagg aactagtgaa gatatagcta tgcttatcgc ttatgagtat
    25981 aaggctgagt taatagtagc agttggtaca cattcaaata tgattgattt tcttgaaaaa
    26041 ggaagaaaag gtatggcaag tactttttta gttagactaa aaataggttc aaagcttata
    26101 gatgctaaag gcgttaattt gttatacaga agtaaattaa aaataaagta catatgggca
    26161 ttaatagcaa cagcattatt tcctgtgttg gtagttgcat cattgtcacc aggagttcaa
    26221 cagttcatac aactaatgca attaaagttt agagtattat tacagatgta aattttagga
    26281 ggataaatat gcatattaat atgaaatatt atatagtcac tataggggct atatttatag
    26341 ctttgggtat aggcatgtta gtaggattta atttaaataa taatcaacaa ttaagtgaac
    26401 aacaagcaaa cataataaat gatttggatg ataagtttga tatcttaaaa gaaaaaaatg
    26461 ataaacttga cagtgactta gcaagtgtaa atagagatta tgaagaggca gttaatttta
    26521 taaataaaaa tgtagataag attttagctg gttcgctgaa tggtaaaagt ataggaataa
    26581 tatctgctaa tgaaaatgac gattatacaa aaaatataga agacataata aataaatcta
    26641 atggaagcat tgcatttaat ataatattaa aagaaaacat aacaaatcca gaaaagttaa
    26701 aggaaatgtc aactaaacta ggtacagatg taaaaaatgc aaatgatgca gttaattata
    26761 taattgacac tcttaaaaag gaagatgcaa gtgacatttt aacatatctt caagaactag
    26821 atgtaataaa gtttaatttt attggagata catatctaaa atatgattcc gttgtaatag
    26881 ctggtggaaa tgatgcaaaa gacagtacaa aacagtttga aaaaatagaa aaatttgtag
    26941 tttcaaaact taaatcagag aataaatatt tagtagaagt tcaaaataca ggagttaaaa
    27001 cttcttatgt agaactatat tctaaaaaca aagttgcaac tatagacaat attgatgaag
    27061 gtataggaag tatatctcta gctattcttc tacaacaagg taatatagtt ggtaattttg
    27121 gtagattgga tactgcaact tcattgttgc catctattaa atagaaataa ttttaaggag
    27181 agaaaaaatg gattcttata taagtataat aataccagct tataatgaag aaagtagaat
    27241 aaaatataca ttagaaagca tattggacat taagcaaata aatgaaataa tagttgtgga
    27301 tgatggttca attgacaata ctgctaaggt tgtatctgag ctagaaaatg aaaagattaa
    27361 attttttaag ttagataaaa atagaggtaa aggatatgct ttaaattatg gtatcaaatt
    27421 agcaatgaaa aatgcagata ttataggttt tctggatgga gatttaggaa gttctgctaa
    27481 agaagttgaa aaattaatag tacctatatt aaatggtgag gcggatgtta caatagccaa
    27541 gtttcctcct gcaaagagaa aaggaggttt gggatttgta aaaggtttag ctaaaaattc
    27601 tgtgtttgag atgactggtg ttgagttaga tgcaacttta tcaggtcaaa gaatatttaa
    27661 aagagaagtt ttagaaaagt ttgatgaaat accttttgga tatggagtag aagttggaat
    27721 gaccattgat atattaaagt atggatttaa aattaaagaa gtattagtaa atatgactca
    27781 taatgaaaca ggaagaaatt taaaaggttt tattcataga gggaaacagt attatcacat
    27841 aaaaaaggtt ttaggacaaa aaaagaaaga atggaggtaa tatcttagtg aaagattata
    27901 tactttatac cattttatta ttaactggat tatttggaac ctatgctgta attccactat
    27961 ttaaaaattt attgataaat ggaaatgtac ttagaccaaa ctataaaaaa gatatgatac
    28021 cagtaagtat gggaattgta tttttgccta tgattgtaat caatggaata atacttgctt
    28081 tctttactac agaatttaag gatttagcgt atatatttat gtttatgttt ggtatgatat
    28141 ccatgttttt tgcagggata ctagatgatg tcattggaaa tagagatgta agtggtttaa
    28201 aggggcattt taaaagttta tttaaaggaa gtcttacaac aggaggtttt aaagctttat
    28261 ttggtggatt tgtagggctt atagtttctg ttgctatatc aaaagacata attgatataa
    28321 tagtaaatac actaataata gctctatcaa ctaatttaat gaatcttttg gatttaagac
    28381 caggaagggc aataaaagca tatttattca taatggtaat tatattttta acattgacag
    28441 gatttgttca agttcttcca ttacttatag taccaaatgt acttgcatac tttaattatg
    28501 atttaaaagc aagggctatg atgggagata ctggttcaaa cgtccttgga atatctattg
    28561 gaatgctcat ttcttttggg tatccattta acattagatt aggatggtta atatttgtag
    28621 ttattatgca tatattcact gagaaatact cattaactaa gataatagaa aaaaataaac
    28681 tattaaattt tatagataaa ttgggaaggt agtattatga taagcaaaag agtggggatt
    28741 gttgaatcaa tagtaagtca aactgaaaca ctagatgata taagagtaaa tataaatgga
    28801 gaaatacaaa gggcatataa ttatcccaaa atatctggaa ctataaatat aggtgacgaa
    28861 gtggttttaa atacaacagc agtagaactg agcttaggta caggtggata ccattttgta
    28921 ataactaatt taaataatat agaatctact ttaactgaag gtggacatat aatgaaactt
    28981 agatatacgc ctcttcaaat aaaagtggat tctgttgagg aacaagaaag tatttatcat
    29041 gataaatttg cgaattttaa aggattagaa ggtctttctg ttgtagtagg gactcttcat
    29101 agtatgttaa ctccatttgc agcttcattt aaaagaaata atccaaataa aaaattagtg
    29161 tatataatga ctgatggagc atctcttcca atttatttaa gtaaaaatgt ggatacttta
    29221 aaagaaaaaa aattaataga tagtacaata actataggaa atgcgtttgg tggagattat
    29281 gaatgtataa atatatatac tgcattaatt acagcaaaag aaatactaaa agcggatgca
    29341 gtatttgtca gtatgggacc tggaattgct gggacaggaa caaaatatgg atttacaggg
    29401 attgaacaag gctctattct tgatgctgta aaaaaacttg aaggaagagc aattgcaatt
    29461 ccaagaatta gttttgctga caaacgagaa agacatcaag gtatttcaca tcatagtatg
    29521 actgtattta aagaaattgt aaatgtaaat gtagacatac caataactat atatgatgat
    29581 gaaaaattga actttataaa agagcaaatc aatttaaatg gtttagacaa aaagcataat
    29641 ataatttata tagataataa taaaacgaaa gaggatttag actattttaa tctaaaagta
    29701 aagagtatgg gaagaaatta tgaacaagat gaggcatttt tcaaagctgc aagcacagca
    29761 gcatattact taatggaggt ttaagatggt attagaagaa aaaactatta gtagtgatag
    29821 ggtttataca ggtaaagtaa ttactttaaa ggttgatact gttgaaattc cagggcaagg
    29881 atatcaaaaa agggagttag tagaagttgg tggagcagta ggaatagttg caatcacaga
    29941 tgataataaa gttgtgttag ttaagcaatt tagaaaacct atagaaaagc caatctttga
    30001 aattccagct ggtaaactag aaaaaaatga aagtccaaaa gaatgtgctg aaagagaatt
    30061 aaaagaagaa acagggtata gtgcaaaaaa tattaagcta atacataaat ttttcacttc
    30121 agcaggtttt tctaatgaga taatgtttgt atatttagca acaggtctta ctccaggaga
    30181 gaataattta gatgcagatg agtttttaga tgtctatgaa attgagcttg aagaagcata
    30241 taacatggta ttaaaaaatg acgttgaaga tgcaaaaaca tctattggat tattattagt
    30301 aaaagatatg tttaaaaatt aatatttata aaatgaataa ccctttgtcc taaagcatat
    30361 tattttataa aggacaaggg ggaattaaat tgagaagaac atatagaaaa aatgatttta
    30421 gagaattaaa tagacaaata attattatag gattgttgtt tatgatgtct attgttgttg
    30481 ggtcttacat aaataaaata ttgccaggtt ctagtaacaa tatattaaac aatataaatc
    30541 ctgctgtaga gtattataat ctaaatataa gcataaaaga tacagtgata caaaatttaa
    30601 aatcagatgc aatctttatg ggctcaatag ctctattaag tctatttgta gtaacaattc
    30661 cagcagtgtt ggtagctttt gtattaaaag ggatgtcaat tggttataca ataaatagtt
    30721 gtatattggc attaaaattt aaaagtataa aaatgatttt aataatatta tttaaaaatt
    30781 tgattattat tccatgtgca atcatattag cattaattag tttaagttat tttaaagaaa
    30841 tggtttatga atttaaaaag aaaaatagaa aaaatatgca atttttaatt aagaggtata
    30901 tactaaatat tataattata atagcactat cattggggtt gcaattaata ttaaatactg
    30961 caagtattgg tattattaag ttcctagcta aataaaggag tttataatgg atattataga
    31021 gggatatata gattacataa aagataaaaa aaaattatca gagaatacag tggcatcata
    31081 ctttatggat ataaaaaaat atatggaata tataaatcaa aaaagcatta aactagtaga
    31141 tatagtagaa aatgatatta taagttactt aataaagttg gaaaaagata atgtatcaat
    31201 agctacaata gcgaggatga tatcctctat aaaatctttt catgattatt tatttttaaa
    31261 tcatatatgt acaaataatc ctgcaaagga tataaaaaaa cctaaaataa aaaaagaaaa
    31321 tataaatata cttacagaag aagaaattga aagactatta aattttccaa agttaacaac
    31381 acctaaattg attagggaca aggctatttt tgaagtatta tatggtactg gtataaaagt
    31441 atctgaatta gtagagatga atatagagga tattgaccta gatattgatt atatatattg
    31501 taattcatgt tgtaaaaatc aaagagtgat accactttgt gatattacaa aactttactt
    31561 agaaaagtat ctaaaagaag caagacctaa aatggctgta gaaggtgaaa aatcattatt
    31621 cgtaagttca ttaggtcaaa ggtttacacg acaaggtctt tggaaagtca taaaaaaata
    31681 ttctaatcta gccaatatag ataaaaatat aaatcctaca atgcttagac attcatttgc
    31741 tattcatttg ttaaatgaag gtgctaacat tgctgttgta agtaaaattt taggaaatgt
    31801 taatttatct agtttgcaag tatatttaaa tcatatagat aaaaacgtga gaagagaaat
    31861 aaaggagaaa catcctagaa atgatgttga tgtagaatta caaaaagcag aagctaaata
    31921 gactatatag caaattttat aatttagaag gagcatattt atatgagcag agtaatttgg
    31981 attgtaattg atagtgttgg gataggagca ttgcctgatg ctgaaaaatt tggtgatagt
    32041 aaggatgtaa gtacccttgg aaatatattt aaggaatatc cagatattca gattcctaat
    32101 atgagaaatt taggtatagg aaacatagat ggtatagatt tttttgaaag tataaaagaa
    32161 ccaataggtt gttttggaaa gtgtaaggaa atgtctcagg gaaaagatac tactacaggt
    32221 cattgggaaa tgactggtat aatagttgac aaaccattta agacatttga acatggattc
    32281 tctaaggaga taatagaaga gtttgagaaa aaaactggaa gaaaagtagt aggaaataaa
    32341 ccagcttcag gaactgtaat aatagatgag tatggagaac atcaaataaa aactggagat
    32401 gtaatagtat atacatcagc tgatagtgta tttcagattg ctgctaatga agaagtaatt
    32461 cccctagaag aactctataa tatgtgcaaa atcgctaggg aaataatgat gggggataat
    32521 gcagtagcta gagtaattgc tagaccattc ataggtaaga aaaaaggtga gtttgtgagg
    32581 acttcaaata gaagagatta ttcactagac ccatttgaac caacagttct tgataatata
    32641 aaagaatcag gacttgatgt tttagcagtt ggaaaaatag aagatatttt taatggaaaa
    32701 ggaattacag atgctatcca cactaaaagc aatatggatg gagtggatga aactttaaat
    32761 tatatgaaac aagataataa aggtctaata tattcaaatc tcgttgattt tgattctaaa
    32821 tatggtcata ggagagaccc agaaggatat aagaaagctc ttgaagagtt tgatagtaga
    32881 cttcctgaaa taatggctaa tatgagagaa gatgatattt taataatcaa tgcagaccat
    32941 ggaaatgacc caacatataa aggtacagac catacaagag aatatatacc tgttatgatt
    33001 tatggcaata agattaaaaa aggttttaat ttaggagtaa aagatacttt tgctgatatt
    33061 ggagcaactg ttgcagatat actaaatgta aagttgccta aacatgggtc aagctttaaa
    33121 gaagatttat tttaaattat aaaatgataa aaacttagct taataggatg gagagattgg
    33181 aatgtttgaa aagatagcac aaagtagtaa atttataaat tctaaaagta atataaaacc
    33241 taagattggt ttgatattag gttctggact aggtgattta gcaaatgata tagaagaccc
    33301 tgtcacaatt aaatatagtg aaattccaaa cttccctgta tcaacagtag caggacatgc
    33361 tggacaactt gtaattggaa aacttgaagg aaaagaagtt attgctatgc aaggtagatt
    33421 tcattactat gaaggatatt ctcagaaaga agctactttt ccagtaaggg ttatgaaaga
    33481 attaggagtc gagattctta tagtaactaa cgctgctgga ggcgtaaata aagaatttaa
    33541 agcaggagat ttgatgctta taagagacca tataaacttt agtggaagta atccattagt
    33601 aggaaaaaat gatgacagat ttggtgctag atttccagat atgtcagatg catattcttc
    33661 taaatatgta gatgtagtta aaaactgcgc taaagaatgt aatatagatg tcaaagaagg
    33721 cgtttatatg ttttttagtg gtccaaacta tgaaacacca gcagaagtga gaatggcaca
    33781 aattttggga gctgatgctg taggtatgtc tactgttcca gaagtcatag tagcttctca
    33841 ctctaatata ggagttattg gtatatcttg tataacaaat atggctgctg gcatcttaaa
    33901 tcaaccacta agtcatgagg aagtaataga aactactcaa aaagttaaat cagaatttat
    33961 gaatttagtt aaaacaacgg ttaaaaattt ataagttaaa gcttattata gattttttga
    34021 ggaagtttat aataaaatag ttaatgttta atttgaaaag aattaataaa aagctgtctt
    34081 gatatagaaa gttatttcta ataaaggaca gtttttttat aatactacaa catttgaaag
    34141 tgttttaaag tttaatattt atttaaatta ttataggtta gaatggagat ttcaattatg
    34201 agaatgtatg atattataaa aaaaaagaga gataacctag agcttagtaa aaaagagata
    34261 aactacttta tagaaaacta ttcaaaggga gatatacctg actatcaagc ttctgcacta
    34321 ttaatgtcga tttatttaaa taagatgaat aaacaggaaa ctgtacatct tacagaagca
    34381 atgatgaact ctggagacat gataaaccta tctgagatag atggaataaa ggtagataag
    34441 catagtactg gtggtgttgg agataaaact accatagctc ttataccttt ggttgcttca
    34501 tgtggagcac cagttgcaaa gatgtcaggt agaggtcttg gtcatactgg tggtactatt
    34561 gataaactgg agtctatacc aggtttttcg acagaaatgg agataagtaa atttattgat
    34621 tctgtaaata agtctaaaat agcagtttgt ggtcaaagtg caaaggtagc aattgcagat
    34681 aagaaaatat atgctcttag agatgttaca gcaactgttg ataatatatc attgatatca
    34741 tcaagtgtaa tgtctaaaaa gttagcttca ggagctgatg caattgtgct agatgtaaaa
    34801 actggtgatg gagcatttat gaagacatta gaagagtctt ttgagttagc taaatctatg
    34861 gttgatatag gtactggtat gaatagagat acaataggga ttgtaacaga tatggatgaa
    34921 cctttaggtt ttgcagtggg aaactcttta gaagttatag aggcaattga gacattaaaa
    34981 ggaaatggac ctaaagacct tgttgagcta tgcgaagtat taggagcata tatgttggtt
    35041 ttatcaaagg tagcacatga ttttgaagat ggtgttgaaa aaataagaga atcaataaaa
    35101 tctggtactg ctatacaaaa actcaaagag tttatagaga atcaaggtgg taataaagat
    35161 gtagtagatg attattcttt attcaaaaaa tcaaagtata aatatccagt taaatcaact
    35221 aaatcaggtt atataagcaa aattaaagct gaagatgttg gtgtatcggc tatgattctt
    35281 ggagcaggta gagaaactaa agatgatatt ttagatttat cagcagggat aattttagaa
    35341 aagaaagtag gagattatgt aaatgaaggt gaagttttag cttacatgta ttataatgat
    35401 gaacaaaagc tttcattggc aaagggaaga tttattgatt cctactctat tgtagattca
    35461 aaaatagaaa aaaataaatt aatatatggt attgtaacta aagaagagat taaaaagttt
    35521 tagaaaattt tagaaaagtg attataaaag gcctaacatg tcaaaaataa tgttaggttt
    35581 tttatttttt gatagactat aaattatacc aaaaatatat attttaaaac attttttaca
    35641 atataaataa gtttattcta caagatgatt gaaaattaaa gtatctagtt ttataataga
    35701 aaatggataa atatacataa ggagtagatt atattatgaa ctttaaagaa aatagattga
    35761 aaatagcagt attaataatt gttattttga ttatcttagc aggtatattt gtgtttatac
    35821 aaataggtcc ttatgataaa aataacaaaa aagatgtaat tatcgacgtt ccaagtggag
    35881 catctgttgg aaaaatatct gacatattat atgaaaataa attaataaag aatgaactat
    35941 tatttaaact tttggtaaag gttagcaata aagctccgtc tataaaatca ggaacatact
    36001 tacttaatca atcgtattca aacaatgata taataagtct tttagtttca ggtaaaatat
    36061 atcaggatgg tataaaagtg actattccag agggagctac atctaaagaa attatagcaa
    36121 tgttagtaag taaaaattta ggagataaag ctacttttga aaatttaata aaaaaacctc
    36181 aagaatttta tgataaattt ccatatttga aggaagatgg aataacctct ttagaagggt
    36241 tcttgtatcc agaaacttat tattttaatt caaagaaaca atcagaagaa gatattttaa
    36301 gcgaaatgtt aaaagtattt gacagtaaat acacggacaa gtttaaaaag aaacaaaaag
    36361 aattaaatat gactcttcaa gaagtaatgg aaatggcatc aataattgag aaagaagctg
    36421 ttcttgacaa agacagacct ataatagcaa gtgtgtttta taatagactt aaggtaggta
    36481 tgcctcttca atcagatgct acaatacaat atatatttga agagagaaag aaaattgtaa
    36541 catatgatga tttaaaaatt gattcaccat ataatagtta taaaaataag ggattacctc
    36601 ctacaccaat atcaaatcct ggtataaaat ctattgaagc agcattatat ccagagaaaa
    36661 cagactattt atattttgta gctaagattg atggtggaaa taattatagt acaaactatc
    36721 aagaccattt aaaatacgtt aaagaatata aagaagcaag ggataaacaa tcaaaagata
    36781 caaaagcaac aaataaagaa aatacaaaaa aataaaccat gattataaaa agtctaacag
    36841 ggtaagaata atgttgggct tttttattga aaattgttat tcttagattt ataataaaga
    36901 aagtgtaaat atatataaca atatgaattt aaaaaataat aaattaatat aaaacttgaa
    36961 tatttattat tttagggagt gtttttatga gtaatatagt taatgatttg gttgaagatt
    37021 atataagagt gacattaaaa gaaaaagaag gatttttaaa agatttagaa atttatgcag
    37081 aggaaaatag tgttcctata atacataaag aagtttcaga tttattaaaa gtcttgttaa
    37141 aggttcaaaa accaaagaga attttagaag taggatgtgc aattggttat tcttctattt
    37201 tttttgcaac tattatagga aaagatgcag atataataac tgttgagaga aatgaaaaaa
    37261 tgatagaaaa agcaaaagaa aacataaaat tagctggatt tgacaataac ataactatac
    37321 tagaaggaga tgcagaagaa aaacttagtc aagtccaagg tgaatttgac cttatattta
    37381 tagatgcagc caaaggtcaa tataagctat tttttgattt agttatagat aaattaaaag
    37441 atgggggact tttagtatct gataatattt tgtataaagg tatggttgct catgatgatt
    37501 ttgttgtaag aagaaaaaag actatagtta aaagaatgag aaattattta gattatatat
    37561 gtaattgtga ctatttaagc acatcattgg taccaatagg tgatggtgta gcactaagtt
    37621 ataaagaaag taaaagaggt gatgtagatg gaattaaata aagtagaact tttagctcca
    37681 gctggtgatt tggagagatt gaaaatagct ataacatatg gagcagatgc cgtttatata
    37741 ggtggagaaa tttttggaat gaggtctgca gcgaagaatt ttagtaagga agatatggca
    37801 gagggagtag cttttgcaca tgaaagaggc aagaaagtat tcgtaacagt aaatataata
    37861 cctcataatg aagattttct acaattagaa gactatttgt tggaactaga agagatagga
    37921 atagatgcag ttataatagc tgacccagga gtgcttagtg tcattaaaaa agtaattccg
    37981 aatatggaaa tacatttaag tacacaagca aatacaacca attatctaag tgcaaacttc
    38041 tggtacgagc atggaataaa aagagttgta gtggcaagag aattatcttt tgatgaaata
    38101 tctgagataa gagcaaaaac acctttagat atggatatag aagcgtttat gcatggtgct
    38161 atgtgtattt cttattctgg cagatgttta ataagcaact atatgactgg aagagatgca
    38221 aacaaaggtt cttgtgctca atcttgtaga tggcaatatc atttggtgga agaaaaaaga
    38281 cctggtgagt attttccaat atacgaagat gaaaggggta cattcttctt taactcaaaa
    38341 gatttatgta tgatagagta tataccagag ttaatcaaat ctggtataac aagtttaaaa
    38401 atagaaggta gaatgaagac tgcatactat gtagctactg ttgtaagagc atacagaatg
    38461 gcaatagatg aattttacag agacccagaa aactggaagt tcaatccaat gtggatggaa
    38521 gaactaaaaa aaggtagtca tagacatttt acatcaggtt tttacttgaa taaaccaaca
    38581 acagaagacc aaaattatca atcagcatca tatgttagaa actatgattt tataggtata
    38641 gttagagaaa cagaagatga agatggtctt attgttgttg agcaaagaaa taaaatgtgt
    38701 gttggtgatg aaatagaagt tatgggacca tataaagaaa ctatgtttac taaaattgaa
    38761 gaaatgtaca atgaagaagg agaagctata gaatcagctc ctcatcctag acaaatagtg
    38821 aagttaaagc tatcagtaaa agttggtaaa gattatatgc ttagaaaagt tatagaagaa
    38881 aaggttgaag aataataaaa aattgataaa aatagtatta gccccttaga aattgtcaat
    38941 aatgaaatta tctaaggggt gatttcatgt caaaaaagaa gactccattt ttgaaaaaag
    39001 tgggaaagag aagctggtgt atatttacta taattttaat aatttatagt gttttaattt
    39061 ataggctagt tgatatacaa gtattaaaag gtgataaata caaacaaagt gttgaatcac
    39121 aaagcgttga gaaagtcgag ttaaatagtg gaagaggaat aatctatgat agaaacaaca
    39181 agaagcttac agatacaagt aaatctcagg tattaattgt tgaaaaagaa aaattaaata
    39241 ataactataa aattttggag cttattaaga aagctactaa gatgaatgat ttagatatat
    39301 ataaagctgt acaagagcaa ttgacacgcc ctataataca gattcagact aaaaatatcg
    39361 ataagagtat gaaaaaagaa cttgagaaaa atggaataat ggtagaagaa aaaactatga
    39421 ggtacgcaaa agatggttta ttatcacata caataggata tataaaagaa gatgataaat
    39481 caggtcaatc aggaattgaa aagagtatgg atagtgtact tagaaattca aatgaaaagt
    39541 atataagtgc ttttaaagct ggagatgcag gaaatgaaaa atctctaaac atactaaagg
    39601 gaagtgtaaa aactgttgat aataaggata aagatagaca tctaaaaact actattgatt
    39661 ataacattca aaaaaaatta gaacaaatac taaataaaga agaaaatcct acagcagcaa
    39721 taatctcgga agcatctaca ggtgaaattc ttgcaatgtg ctctagacct aattttgacc
    39781 aaaatgacat atcaaaaagt ttaaaaggaa aaaatggaga atttgaaaat agagttataa
    39841 aagcgacata tccaccaggg tctgtattta agatggtagt tttattttct gcacttgaaa
    39901 atggtgttat agatgagaac tatacttaca actgtacagg taagactaaa gttgggaata
    39961 ctaatgaaat actgcgatgt aataaaagag atggtcatgg tttccaaaac ttaagacaag
    40021 ctttttctaa ttcatgcaat cctgcatttt tagatatagc tatgaaactt ggaaaagaaa
    40081 agatactcaa atctgctgaa aaattacatt tatttgaaaa ggttgatatt ggtcttgatg
    40141 aagaaaaaat tagagaagct ccaaaaaaca tatcaataag aaatttagct ataggtcaag
    40201 aaaatataga atttacacca cttcaaataa atcaaatgac tcaaataata gctaataatg
    40261 gaacattcaa accactatac ttatataaaa gtcttgtgga caataatatg aatacaataa
    40321 aaacttataa gtcttcaaaa aaagaagaac ttatttctcc ttatgtgtgt acacaagtta
    40381 aagaatatat gaagtctgta tctaggattg gtacagctaa agacctaaaa gatattgaag
    40441 gtggttgtgg agttaaaact ggtacagctc aaagtagttt aaataaaaaa gctatagacc
    40501 atggatggat aacaggattt tatcctgaag aaagacctaa gtatgtaata actgtgttag
    40561 tagaaggaac tcaaaaagga aataaatctg caacacctat atttaaggaa atatgtgaaa
    40621 gtataaaata aattatgtac tataaaattt aaggatatat cgtaactttg attttagata
    40681 gaaaattttg tctaatgatg tacgatatat ccttttaaaa tatagtatta actttcttga
    40741 acaagctctt tttctagttt tttaagagct tttttttcga ttctagatac atatgacctt
    40801 gatatatcga gtttttgtgc aatttctctt tgtgtcttat agccataagg agttaaccca
    40861 tatcttaact ggacaatttc tctttctcta ggagtaagaa ttttgtttag ttgttcgtaa
    40921 agttttccaa cttgaacttt tagttcaact tcatcaagaa catagttagc ctctgtacca
    40981 agtatatcta aaagacttat ttcattacct tctttatctg ttccgatagg gtcttgtaga
    41041 gatacttgag ttttattttt tttgttagat ctaatattca ttaaaatctc attttcaata
    41101 catctagaag catatgttgc taatcgagtt cctttatcta tgttataagt ttctatagct
    41161 ttaattaatc ctattgtacc tatggatatt aaatcatcct gatcttccgt tgaattatta
    41221 tatttctttg ctatataagc tactaatctc atgtttcttt caattaatgt atcttttgca
    41281 cttttatcgt tttctatttt aaatttagtt aaatactcaa tctcttcttc aggggttaag
    41341 ggtttttcaa aagatttaag agctgccaat aggtcacccc cttttcaacg gtcactttaa
    41401 attatatgaa ggagacttat ccaagttgac tgttatctaa taaaataata tgcatatttt
    41461 tagaatttaa aacatatatt tgcaaacttt tgaatggaat atgacataaa gagtgggaat
    41521 ttaaatttaa gtttttgttg tatatatatg aaataaggcg aagaaattat tgatttataa
    41581 agtataaatt ggaattgagg tgtcaataaa tataaaagga aatagtattt atatatgttt
    41641 tggtgttgaa gtaaaagatt aatgcaaagc attagagtct ataaaaatat gaatatagga
    41701 ggtgtaattt tacaatactt aaaaaagtaa atgtaattaa ttgtttgtat aaagttgatt
    41761 acaagcaaaa gtatcagtca tagttaatac tgttaagggt gaaggtggtg tgttaattta
    41821 tatatgtatg ggaagaaatt ttgattacaa tgaaaaaact tacaaaagat atttatgaaa
    41881 aaacttatag ttaaatgttt atatataaaa tattatttag gggttgtttt tattattgaa
    41941 aataagaaag agtggtttta tatcaattga atgtataata agtattgcta tattatatgt
    42001 ggctgtttat ttagtttcta catcattgta taattgttat agttttatca gtagaaatat
    42061 atctgacaga gaaatgttaa gtacagcaaa aaaatatata gaagatgaga agtatagaat
    42121 acaaaatagt aagtatgagt taattgaaga taagatagaa aaaaattaca taaatggata
    42181 tgaaattaac agtaaaatag agcaaatttt agattattat caatgctatg aaataaatat
    42241 agagataaaa aatgaattta aaaaactgag gtttaatagc tatgttacta gaaaataaag
    42301 gctctatatt aataataaca ttgataatat tttctattat aagtactgtg tgcattatgt
    42361 gtataggttt aatttataca cataataata tgtttgagtt aggatacaag gacattcaac
    42421 taaaagaaat ttctttaagt ggaatagaaa tatcagtaag taatgtactt tcttgtgttg
    42481 aggaagctat tgatgtatct aataatgaag acgaatttag agaatatttc ttaggaaata
    42541 atatgattga atgtataaaa aaaataaaag atacatctta tagtagttta gaagaacttt
    42601 ctttaaaagt agaaaataat actatttatg acagaggaga tttttttgaa tttgatatag
    42661 aaagtaatgt tagagaaaaa gaatttttaa aagcaataaa agtaagagtg aaaatcaaaa
    42721 atccatggat aggaattgac attgaagatt atatggataa atctaataac aataaaattg
    42781 atgacaaaac attcaataat tataaagcat ctgaaaataa tgaaattcta aatcaattta
    42841 aaaatgaaag tgaaaatata aggaatcaag aggttggtgg atttacagaa gaagatgaag
    42901 aatatgaaga agaacttaaa caaaacataa ttgaaaatat aaagaagaat tttgatgaaa
    42961 aaaacctagt acaggtatat aagtataggg gtgtataact ttgaaaaaag aaggaatgtg
    43021 actgtaatag aattaatagt atctatcagt ataatactct tgatagttac attatctttt
    43081 ccaaaagata atcttgaaaa tcataaaata aatttatttg cacgtcaact ttgttcagat
    43141 ataaggtatg ttaggcgaat taatatgttt agcaactaca atacatatat atattatata
    43201 aatcaaggta aacacgaagg ttatgtattg agaaaagatg gtaaaaacat taaatcaata
    43261 atgctaccta aaaatgcaaa aatagctagg agtattgaaa taataaagtt taaatctgat
    43321 gggtcgcctc aaaaggcgtc tacaatagag atatataata atagattaag aaaaactata
    43381 acaataacac ctgtaagtgg aagagtactt ctgaaagagg gaaaatatga aacataaata
    43441 tggttacttg cttttggaaa gtgtcgtatc attgtcttca atggtgataa ttatactagt
    43501 tttatattct atatttttgt ctacaataaa cttaaagtta aaagtagaag ataagataga
    43561 actacaacaa caaagtcttg aaatcataaa atctatggaa ggaattatta gcaattctat
    43621 gggaataatg aatgtatcta attatgaaga aacatttaaa aagacaacat ctataaaatg
    43681 tagatattta gatgaaaata ataatgaaga aggcataagt aataaagaaa ttatattgaa
    43741 tgaaagacga aataagttat ttgtaaattc tttaaatggc gaaagtagtc aagcaggtgg
    43801 ttatgaaatt ggagattatg tagatgagat gtatgtatca attactaata atggtcaata
    43861 tgttaatatt aaattgaaat tatcaaaacg tagtcaaaaa tatgagactg atttcaaaat
    43921 aaaagtatgg aattttagtg aaagtatata aaaatacata aatttaagag aaagaagtga
    43981 catcaataag tcatataaaa tataattatt gagaactata taactaaata atatcaatat
    44041 gtaaagtgta tagattaaga atatagagga taataataaa aatatagaat aagtaatttg
    44101 ccaaatactt gtttatattg tataacttat gatattatga tattagtaaa aatttgtaat
    44161 cctgacttga cagaattcaa gtaagagata taattttaaa ggcatagtaa tgtaaaattt
    44221 actaaaaaat aagaaattta ttttaatatg gaggaacaaa aaatggtatc agcaggtgat
    44281 tttagaaaag gtgttacatt tgaaaaagat ggacaaccat gtttagtagt tgattttcaa
    44341 cacgttaagc caggtaaagg agctgctttc gttagaacta aatacaaaaa cttaaaaaca
    44401 ggagctataa gagaagaaag tttcaatcca agtgaaaaat tccctaaagc tgttatagat
    44461 acaagacaaa tgcaatatct atacaatgat ggtgagttat attattttat ggatcaagaa
    44521 aattttgaac aaataccatt aaactatgag caagttgaag atgcaattaa gttcttaaaa
    44581 gaaaatgaag ttgctacaat aagattttac caaggacaac cattccaagt ggaagcgcca
    44641 aactttgcag agctagaagt tacagataca gagccaggta taaaaggtga tacagcaagt
    44701 aatgtaacta aagcagctac agttgaaact ggagcagttg ttcaagttcc attatttata
    44761 aatactggag ataaagtaaa gatagatact agaacaggtg aatatttatc aagagtataa
    44821 aaatttatgt atataaaagc ttatatgtct taataatata agatatatga gcttttagtt
    44881 tatatataaa aaagaaagcg aggggtgtta catgaaacat ttatatgaag aagttgcata
    44941 cttaaaaggc ctagcagaag gactagaaat aagtgctgaa agtaaagaag ggaaaatgat
    45001 acataaaata gttgatgcat tagaagtatt tgcagatgct attgtaacat tggatgaaga
    45061 acaagaagaa cttcaagatt ttgttgaatc tatagatgaa gatttagcag atttagaaga
    45121 agatattgct gacatggaag aagatcttta tgaagaagat gacgatgagg atgatgagga
    45181 cttcagctat atagaaatgg aatgcccaaa ttgtggagag ttagtagaaa tagacgaaga
    45241 tttgttatat gatgatgaag tagatgttgt ttgtcctgat tgtaaggctg tgatattatc
    45301 atcagaagat gattgtgatg atgattgtac atgtggtggt tgttcaagtt gtgatgatag
    45361 agagtaaaaa ttcataagaa ttaaaactgc ctatgatgcc tatgagatat tttgcttcat
    45421 aggtagtttt atttttatga ggaagggtat tatgtatttt ggttttgcaa tacttggaca
    45481 aaaatagtac actagaattt atagaatagg attttggagg gatgtaaata tgaaaataag
    45541 taaaaaatta ctagataata tacaaagatt tgaaaatgtt ataaattata aatttaaaaa
    45601 taaagaatat atactagaag ctcttactca tagttcatat tctaatgaaa ataaaaaata
    45661 taattttaac gaaagattgg aatttttagg ggattcggtt ttaggcatag tgataagtga
    45721 ttatttattt aatgaagaag ctaatctgcc cgaaggggaa ctaactaaac ttagagcaaa
    45781 tatagtttgt gaagattcat taagtgaagt agctaatgat ataaatttgg gtattcacat
    45841 gttattaggt agaggagaag aagcaacagg aggaagacat aggacttcaa tattagcaga
    45901 cgcatttgaa gctgtaatag ctgctatata tcttgatgga ggttttgaaa gtgcaagaca
    45961 gtttatactt catcatatgg aaaatataat ttatgattca agaaaaggaa atatatttag
    46021 agactacaaa acgcatcttc aagaggtgtt acaagggaat ggagaaaata atatctggta
    46081 caggcttata gaagaaaaag gaccagacca taataaaaga tttgtaatgg aagttggaat
    46141 taatgacgat gtacttggta taggagaagg caagagtaaa aaagaagctg aacagttagc
    46201 tgctaaaata gctttaaaga aaaaattatg ggaaaaataa atataatatg taaaaatata
    46261 aaaagaaata tttatattag aaggatgttg tagatgaaaa agagaattat acctatattt
    46321 gttcctcaca aaggatgtcc tcatgattgc atattttgta atcaaaaaaa aataacaggt
    46381 gtgagtactg atgttactag tgaagatgca agaaatataa ttgaggaatg tttagagact
    46441 atagataaag atgcagatgt agaaatagca ttttttggtg gtagctttac tgctatagat
    46501 attgatatac aaaaaagttt attatcagtt gctaaagaat atgtagaaaa aggtcttgta
    46561 aaagatatta gaatgtctac aagaccagat tgtattgatg aagatatatt aagtatgtta
    46621 aaagaatata agacaagtat aattgaacta ggagtacaat ccttagatga aaaagtatta
    46681 ttagatagta taagaggtca tcaatctgaa attgtataca agagttcaaa gatgataaaa
    46741 aatagtggaa tcaaacttgg attacagatg atgattggtc ttccagctga cacagaagaa
    46801 aagtgtatat ttacagcaaa aaaatttgta gagctaaaac cagattgtgt tagagtctat
    46861 ccaactttgg tagtaaaaga tacaggtctt gaaaagttaa tggaacaaaa taaatataat
    46921 ccatttactc ttgaagagag tgtaaatata gtaaaaaaag ttcttgtact gttttatgtt
    46981 aataatatca atgtaataag agttgggctt caagctactg atgatataca aattggaaaa
    47041 gctgttttag caggaccata tcatcctgca tttagagagt tggtagaagc agatatgata
    47101 aaagattatt tagaatttgt tattttgcaa aataaaaata taaagcaaat gttggtcaaa
    47161 tctaataaaa agaatatttc taaaatcata ggaaataaaa aaacaaatgt taaatatatg
    47221 gaagaaaaat ttggtgtgct tttaaaaaca caagaatcag atttagatat aaatcaatta
    47281 gaaattgtgt tagatgggaa aagtttaatt agtgctaata tgagagacat tcatagaaaa
    47341 ttgtatgaca tttatgacct ttagttttaa taatgaaggg agagaaaaga tttgtattta
    47401 aaaagactag agttaaaggg atttaaatcc tttccagtaa aaacagatat tatttttaaa
    47461 gaaggaataa cagccatagt aggaccaaat gggagtggaa aaagtaatat atctgatgct
    47521 gtaagatggg tattaggaga acagagtatt aagagtctta gaggtgataa actcgaagat
    47581 gtaatatttg caggtacaga tacaaaaaaa cctatgaatt attgtgaagt ggctcttact
    47641 attgataatt ctgaaaatca attggaatta gattttacag aagtaactat aagaagaagg
    47701 gcttatagaa atggtgaaag tgaatttttt ctaaataata aaagttgtag attaaaagat
    47761 ataaaagaag tctttttaga tactggtatt ggaaaagatg gttattcaat catagaacaa
    47821 ggaaaagtag atgaaatatt aagtaataat cctttaagca gaagaaaagt ttttgatgaa
    47881 gcttgtggca tatcaaaata tagatataaa aaacaggaag cagaaaggaa tttaagtaat
    47941 acaaaggaaa atttagaaag aatagatgat gtatatatag aaattgaaaa tcaattaaag
    48001 cctcttttta atcaacagac aaaggcaaaa aagtacttgg aaataagtga aaaattaaag
    48061 acacttgaag taaatagctt tataagagaa attgaaggaa tagaaaaaga attaagtgag
    48121 gttaatgaac atcgtaaggt tattgaaaag gagctcaatg aaaaagaaga acaaaaaaat
    48181 gttgtagaga aaaagcaaga agatattaat aaagaggtag aggtattaca agatgtaatt
    48241 gaaaaatctg tagattatat aaattcaatt aaaggagtta tatcaaaaaa agaatcccaa
    48301 ataaatttaa ttaaggaaag aataagaaat tttacgaatg aaattagtag aaaaaattta
    48361 gaaataaatg atataaaaga gaagctaaat gaaaataagc aatacataaa agaattagaa
    48421 agtaataaat tatcaggtag tgaagaacta tctaccttgc aggaaaatat aaaagtattg
    48481 gaaggaagta aagataaaca aaaaattaag ttagaatccc taaataatga gatagaatta
    48541 ttgaaagaaa gtataattga tatattaaat aagaaacaag agtttagtaa taagctgtct
    48601 actttaaacg caaataaaga aaatatgaat atcagagatg aaaatataaa ttcagagata
    48661 acagagttaa acaaaaatat tgaaataaaa tcatctgaat tagatactat aaataaagaa
    48721 tttaatatgc aaaatgaaaa tttaaagaat gtaaataata gacataaaga attatcaatt
    48781 aatttgcagg attcaattag tgaacacaat aaactagaag atgaaataca gaaaagtaaa
    48841 tataatttaa atggatataa ttcaaagtta aatgtctata tagatatgga aaatcattat
    48901 gaaggattta atagaggagt aaaagaagta ctaaaaaata aaaatttaaa aggagttcat
    48961 ggagcattag gacagattat aaatgtacct gaaaagtatg agaaatctat agaagcagct
    49021 cttggtgctt atatgcaaaa tataataact gataatgagt ttagtgctaa gtctgcaata
    49081 aactatctaa aacagaataa tttaggtaga gtaacatttc ttccattaaa tataattaaa
    49141 tcaaataaaa taagtctagg aaatttaaaa gcaaatacta agttcatagg aatagcaagt
    49201 gatttaatta catttgatga aaagtataga aatataattg aaaatatact tggaagaaca
    49261 atacttataa ataatataga tgagggcata aaatttgcaa aagaaactgg tcataggttt
    49321 aaaatagtaa ctttagatgg tgaaatctta aatccaggag gttctttgac tgggggaagt
    49381 ctaaaaacta atgggaatat actatcgaga aaaagataca ttaatgaata tacagaaaag
    49441 ataagtaata tcaagaatga aatttcacat ctagaattaa aaagagaatc tttagacaaa
    49501 gatgtgaaaa atataaaaaa tgaaattgat tcacatgaaa gtaaaataaa agatttagaa
    49561 aaaagtataa taataaaaag tacaagtata aagaatgttg aatctgaaat tgaatcgtta
    49621 aaaggtagta ttactaaatt agaaaatgag aagaatgatt taaattctaa cctaaattat
    49681 acacttgaaa aaagtgatga tgttagaaaa gatatggaag agttagatga tttatacaat
    49741 aaaaataaag aaaaaattga tgcattgaat gaggaaataa aaagatacaa tgatttatat
    49801 gataaagaaa aatctgaatt tgatgaattg aacttaagtt tagtgaagaa aacagaagtt
    49861 tacaatagta tagttagaga tataaaaaga atatctggtg aaaattgtga gttagaagaa
    49921 aaaaataaac aattagaaga gtcattaaat tatgaagaac atgaaataat taaattacaa
    49981 gattctatac tcactgaaga aaaagaaaaa gaaaatttaa caaaacaact aggggatagt
    50041 aatagaaatc ttgaaactag aaaaatagca aaagatgatt taaaaaatag ttttgatgaa
    50101 attaataaag aattaaaaac tatagataga cagcatatag aacttaaaga aagtttattt
    50161 aaagttggtg gaagattaga aagattaaaa actagtcaag acacatatat aaataagttg
    50221 tttgagcaat atgatatgac cttagtacaa gcattggaaa ttaaagatga agatttggat
    50281 atagatagaa aattcttaga aagtctaaag agagaaataa gaagtcttgg aaatataaat
    50341 atagactcta taaaggaata tgaggaaata aaagaaagat acgattttta tagcgaacaa
    50401 aagcaagatt tagaagagtc tatggaagag atagaaaaac ttatacatac tttagaagaa
    50461 aatatgaaat ctgaatttga aattaagttt gaagaaataa gtaaaaattt taaatatgta
    50521 tataagagat tatttggtgg aggttgtgga gaattaacta ttttagataa agaaaattta
    50581 ttagaaagtg atatactaat aacagctcaa cccccaggta aaaaaatgaa aaatttaaac
    50641 ctattatctg gtggagagaa agctttaacg gcaataagta tattatttgc tatattgatt
    50701 acaaagccaa caccattttg tatattagat gagattgaag cgccacttga tgacgcaaat
    50761 atatttagat ttggagaatt tttgaaggat ttatctaaag agactcaatt tatatcagta
    50821 actcatagga gaggaactat ggaagctgct gattatatct acggagttac aatgcaagaa
    50881 aaagccattt caaaagttat tagtttaaaa ttaaaagaag ctcaggaaat aacagatatt
    50941 atataactta gattgacata ggaggaataa tagtgttaaa aaaattattt ggttttggaa
    51001 aagacaagga aaaggaaata gaaaaaaaag atgctgaaga agaaatagaa gttgaagaca
    51061 gtgttgataa tttagaaaac ttggaagaaa ctattttttc aggacttgaa gaagaagtga
    51121 tagataaagt ggaagatgtt gaagaaaaaa atgaggaaag tgtcaataat gaaggcactg
    51181 aagaaatcga aaactttgaa aaaatagaag ttgagatgaa tagtaatgga aaagaagtag
    51241 aaaatatttc aaataatgat tctcaagaag agataatcga agaattagaa aattatgatg
    51301 aaattgagtc taaagagata gaagataaag aagataagaa agttaacttg tttgaaagac
    51361 taaaacaagg attgacaaaa gcaaagcaag gaataactga tagaatagat gaagttttaa
    51421 aatcatatac aaagatagat gaagaattac ttgaagattt agaggaaatt ttaataacag
    51481 cagatgttgg tgtaaataca actatggata taattgaaag actaagagat aaaataaagc
    51541 aaaaaggtat tactgaacca ataaaggtaa gagaagaact taaatctata gttgaagata
    51601 ttcttacaaa tgaaaactct acactagata tagaaccagc accatgtata atattaatgg
    51661 tgggtgtaaa tggggtcggt aagactacta caataggtaa actagcaaat agatacaaaa
    51721 aagatggtaa aaaagtttta ttagcagcag cagatacatt tagagcagca gcgacagagc
    51781 aattagaaat ttgggcaaat agaaccaatg tagacataat aaaacatcaa gagggagcag
    51841 accctggagc tgtagtattt gatgctataa aagctgcaaa agctagaaaa acagatgtat
    51901 taatatgtga tacagcagga agattacaca ataaggctaa tcttatgaat gaacttggaa
    51961 aagtatttaa aatagtagat agagaatttc cagaagcaaa acgtgaagta cttcttgtgg
    52021 tggatgctac aacaggtcaa aatgcagttg ttcaagcaaa gacatttaaa gaagttgcag
    52081 atataacagg tatagtactt acaaaacttg atggaactgc aaaaggtgga gtagtccttg
    52141 cggtaaaatc agaagtagat gtacctgtaa aacttattgg agttggagaa agtgtagaag
    52201 acctacaaga tttcaatgca aaatcatttt cagatgcatt atttggtaac taatgttctt
    52261 ttaaaaataa tggttgacaa gagtgttgtt atgatataaa atacaatagt gtaaagtaaa
    52321 taacctttac agtatactgc taggaagtgt aagatatgaa tatagaaaaa atggtcgaaa
    52381 ttggattgtt atttgaacag tataaagaat tattaactga caagcagaaa gaaatagttg
    52441 ctctatacta tgaagaagat tattctttag gtgaaattag tgaaaatctg aatgtatcaa
    52501 gacaaggtgt ttatgatacc ttaaagcgtt cagaaaaaat actaagagat tatgaagaaa
    52561 agttacactt agtatcaaag attcaagagc aggaaaaaaa tattaagatt ataaaagata
    52621 aaattattga cattaaagaa gatttattgc acaatagaga ttgtgctaat ttaatcccta
    52681 agctagaaaa tatagaagat gtatgtaggg agatgataaa atgatatttg aaggattgtc
    52741 tgataaacta caaggggcat ttagtaagtt aaaatctaaa ggaaaactta cagaagtaga
    52801 tgtaaaaaat gctatgagag aagttaaact tgctcttctt gaggcagatg ttaactttaa
    52861 agtagttaaa gatttcataa aaaaagttca agagagatgt gttgggcaag aagttatgca
    52921 aagcttaact ccagctcagc atgtaatcaa aattgtaaat gaagagctta caagtttaat
    52981 gggagatgta caaagtaaag taatgatttc ctcaaagcct cctacaattt taatgatggt
    53041 tggattgcaa ggtgcaggta agactactac agcaggaaaa cttggaggat attttaaaaa
    53101 gcaaggtaaa aaacctctat tagtagcttg tgatatttat agaccagcag ctataaagca
    53161 acttcaagtt gttggagaaa aattggatat acctgtattc aatatgggag ataaagaaaa
    53221 tccagtaaat attgcgaaag caggtttaag ccatgcgatt aaaaatgcta atgatttagt
    53281 tattatagat acagctggta gacttcatat agatgaagta cttatggatg agttaaagtc
    53341 tattaaatca gaagttaagc cacatgagat acttttggtt gtagattcta tgacaggtca
    53401 agatgctgta aatgttgcag aaagcttcaa tgaggcactt ggtgttgatg gagtagtatt
    53461 aactaaattg gatggagata ctagaggtgg tgctgcactt tcaataagag cagttacaca
    53521 aaagcctata aaatttatgg ggatgggaga aaagctagat gatatagaac ctttccaccc
    53581 agatagaatg gcatcaagaa tattaggtat gggagatgtc ttaagtttaa tagaaaaagc
    53641 acaagaaaat atagacttag agaaagctaa agaattagag caaaaaatta aaaaacaaga
    53701 gcttgatttt gaagatttct tacaacaaat ggaacaaata caaaatatgg gacctcttga
    53761 caagatactt ggtatgatac ctggtatggg aaatgtaaaa gaccaacttg gagatgtaga
    53821 tttgaatggc aaagaaatga agagaacaaa agccataatt cagtcaatga ctacatatga
    53881 gagaagaaac ccaggagtat taaatgcttc aagaaagaaa agaatagcta aaggcagtgg
    53941 aactagtgta caagatgtaa atagactcat aaaacaactt aatgaaatga aaaaaatgat
    54001 gaagatgttt gctggttcac agaagagtat gaagaaaagg ggcggcttag gcggtttacc
    54061 tttttttaaa taaaatataa aaaagtaaaa attattcgga ggtgactgta atggcagtta
    54121 aaataagatt aaaaagaatg ggagctaaca aaaaaccttt ctacagaata gtagtagctg
    54181 attcaagagc tccaagagat ggaaaattta tagaagaaat aggatactat aatccaattt
    54241 ctgaacctaa acaagtaaga ataaatgatg aaaaagctat aaaatggtta gctactggtg
    54301 ctcagccaac agaagtagtt aaaaaattac ttgtaaaaaa cggagtaata gaaaaattcg
    54361 aagcttctaa acaagcaaaa taattttttc ttggcaatta gtttaagtaa gggctcattg
    54421 ctaaggttac tttaggagat gaaatatatg aaagagctag ttgtagatat agctaaggct
    54481 ctagttgata atcctgactc agttgttgtt gaggaatttg aagataatga tggtatcgtc
    54541 ttaaagctta cagttgctca agaggatatg ggtaaggtta ttgggaagca gggaagaata
    54601 gctaaagcta taagaactgt cgtaagatct gttgcaaata gagaaaatat aaaagtttct
    54661 cttgagatag tataaaatag gaggattagg tataatgcct agtccttttt atataaatgc
    54721 gtagtttaag attttatgat gatatgacca cataagatta aattttttaa gaaggtgata
    54781 acatgaaaga aaaattaact cattttaagg taggtcaaat agtaaataca caaggtttaa
    54841 aaggtgaagt aagagtttat ccacttactg atgatataga gaggtttgat gaattaaaag
    54901 atttctatct gggaaaagat ttaaataata aattggctgt tgaaaaagta agatacaaag
    54961 gttctatggt tataatgaag ataaaaggca tagacagcat agagaaagca gaaaaactaa
    55021 aaaataaatt tatgtatgta tctagagaag atagcagaga gcttgaagaa ggcgaatttt
    55081 ttatagcaga tatgattgga atggaagttt taactgtaga tggaaaatat gtaggaatac
    55141 tagaagatgt tcttcaatac tcagcaaatg atgtctatgt agtaaaaggt gaggaagata
    55201 aagaatttat gataccagct ataaaaaaat ttgtacctac aatagatatt gatgaaagaa
    55261 aaatgattat agaccctatt aagggaatga tagattaggt ggtaatatga gatttcatat
    55321 aatgacactt tttccagaaa tatttaattc gtacatggat gaaagtataa tgaagagagc
    55381 agttgaaaaa ggtataattg aggttcatat atataatata agggattttt ctaataataa
    55441 acataaaaaa gtagatgatt atccatttgg aggaggagcg gggatggtaa tgactcctca
    55501 acctatatat gacacatata agcatataat aacaactcat aatataaata acccaagtgt
    55561 aatttatctt actccaaaag gtaaggtata caatcaatct atggcaaagc agatgtcttt
    55621 aaaagaagat ataatactac tatgtggtca ttatgaagga atagatgaga gaatcattga
    55681 tttaatagtt actgatgaaa tttcaattgg agattatgta ttaactgggg gagagctccc
    55741 agcacttata atgatagatt ctatatcgag attaatacca ggtgtattaa accaagaaga
    55801 atcatttgaa gaagaatcat ttaaagacaa cttattggaa tatcctcatt acactagacc
    55861 tagagatttt gaaggattaa aagtaccaga agtgctttta tcagggaatc ataagaagat
    55921 agatgagtgg agaagagaag aatctataag gataaccaaa gaaagacgat ttgatttata
    55981 taaaaagtct aatgaaaaat aaaaatataa tgaaaaatta atatatatgt atacaataaa
    56041 gtacctataa atggaattaa aaacataaaa gtgcttgtaa taactattga agtatagtat
    56101 aatataaaat gttgaaaata cgggcgttcc tctgtttaca ctggtcgggt agttaagaac
    56161 gtctatgata tctaggagga agaaagatga atgaaatgct aagagcaatt gagcaagaac
    56221 aattaaaaaa tgaagttcct aattttggac ctggggacac agttaaagta cacgttagaa
    56281 ttatagaagg aaaaagagaa agaatacaag tattcgaagg tgtagtatta aagagacaag
    56341 gtggaggagc aagagaaact ttcacagtta gaaaaatgtc tttcaatgtt ggtgtagaaa
    56401 gaactttccc agttcactct ccaaaaatag aaaaaataga agttactaga aaaggtaaag
    56461 taagaagagc taaactaaac tacttaagag gtagagtagg taaagctgct aagataaaag
    56521 aagctagaaa taaataatta aaaactttac ttataaatgg ggggctgatt tatcagtcct
    56581 ctttctattt taatgaaaat tacagcaaat aattatacta gaaataaata gaaatatttg
    56641 ttgaaaatat acattattta tctatgaatt tatagctttt aaaatgaaaa ataaaattgg
    56701 taaatattgc attatatttt gtaacaatat taaaaataag agaacagtat tatgtaaagt
    56761 atgacgttaa aaagatgaca atattatagt ttgttaagat agatttttta agtggaatta
    56821 ggatataata atataatatt aataaggagt gatacatgtg tcaaatgaat acgaagagta
    56881 tcttatgaat gacaatctac atataaactg gtatccaggt catatgaaaa aaactaagga
    56941 gttagtaaaa aataacttaa aattgattga tgtagtaatt gaacttttgg atgctagaat
    57001 cccgtttagt agtaaaaatc cagacataga caggcttgta ggagataagc caagagttgt
    57061 cgtacttaat aagagtgata tggcagatag ggataaattg aatcaatgga ttgaatacta
    57121 taagaaaatt aatatcaaag ctataccagt agatacaata aaaggcgttg gtataaataa
    57181 gataatcgaa gaatgtaaaa atgtaactag agaaaaaatg agttctttaa aagacaaagg
    57241 gagaaaagaa agagctatac gaataatgat agttggagtt ccaaacgttg gtaaatcgtc
    57301 tcttataaac aaattaacag gtagaaaaag cactcaaact ggagataagc caggagtaac
    57361 gaaagggaaa caatgggtta gattaaaggg aaatcttgaa cttttagata cacctggaat
    57421 actttggcct aagtttgaag accaagaagt tgcattaaat ttagcattta gtagagctat
    57481 aaaggatgag attttagata ctgaaacttt ggctttaaga cttatagaaa aacttatgaa
    57541 aatagaacct gagaagttga aagccagata taagttagat tgtctaggag aaactccaat
    57601 tgaaactatg gatatgattg gccataaaag agggtttata acaggtaaaa aagaactaga
    57661 ttatacacgc atagccacaa ctgtattaaa tgaatttaga gatggaaaaa taggaaatat
    57721 aacattggaa gttccagaaa acgttaaaag atagatttta aaacaaaatc aaaaatttaa
    57781 tgttaaaaat ttaaaaaagc cttttagcaa aggctttttt atttttttga aatatagaca
    57841 aatatctcca tataaaagta taaattaggt tagaaaacct ttaaaaatat taatttaaga
    57901 caaatatacc taatattttg aaaattaaaa tgagtgtgat tatttaaaat tttagcatat
    57961 tgacttgctt gtttcgtata atatataata agcacaaata aatacaatat attaaaaatt
    58021 taatttattt aattttatta atttaataat aagttcttat tgtttaattc aacttactta
    58081 tgcaagcgta aacaatcgag acattttatt aatttaataa ggttaaaaag ggggatttta
    58141 taatgggtaa gaccaaggat gttatagtat ttggatttgc gttatttgca atgttctttg
    58201 gagcaggtaa cttaatattt ccaccttact tgggaataat tacaggtcca gaatggttaa
    58261 ttgccttctt aggatttacg tttgctgatg ctggattggc acttctagca gtaatggcta
    58321 cagctaaatt cgatggaaac gtagttgaaa tgtttaaaag atgtggtatg aagttaggaa
    58381 ttcttatagg ttgtgcagat attttatgta taggcccatt cttagctata ccaagaacag
    58441 gtgcaacaac ttatgaaatg ggtataatgc ctttgtttgg aacttcaatt ccagtgttat
    58501 tattctgtat actttttttt gcgatttcat atgtactaac aataagacct tctaaggtcg
    58561 tagatatagt tggacagttt ttaactccag ctcttctaat agctttagca tttataatta
    58621 taaaaggtat tatatcacct cttggagata tagtagataa acctatgata cctaatgtat
    58681 ttgcagaagg tataggtcaa ggatatcaga caatggatgc ttttgcagca atagcacttg
    58741 catcagtgct aatagtatct ttaaatgata aaggatattc tactattagt gacaagttga
    58801 aaatgattgg taaggcgggt gttcttgctt gtggaggatt agctctagtt tatggaggat
    58861 tatgtttcct tggggcaact gtatcaacaa tgtatggaac agatgcagta caatcacaag
    58921 taatagtaaa tataactgaa ggattgcttg gaaatgttgg gaaagcgatt ttagcagtag
    58981 tagtatcatt agcatgttta actacatcta taggtcttac atctgccaca ggtcaatatt
    59041 tctctagact tacaaaaggt aaattgagtt atgaaaaaat agtattagct gtatctgtat
    59101 tcagtgcagt agtagcatct tttggtgttg gaactataat aaagatagca tctcctatac
    59161 taagtatagt ttatccacca tcaattgttt taataatttt agctttcttc aatgaaaaaa
    59221 ttaagaatga taatgtatac aaaggagcag tatatatgtc attgcttgta agtatactaa
    59281 cagttatatc tagttatgga gtagctgttc cagtagtaaa ttctttacct cttaattcat
    59341 taggatttaa ctgggtggtg ccagttataa tagctggaat aattggtaat tttataccat
    59401 caaagagtca atctaataca cttggaacaa actaagtttt gtttaattaa aatataatca
    59461 attgtaaata aatgagttaa ctaaaagtaa aaggagttat ttgtacaaat aa
//
LOCUS       DAMJYC010000011        59486 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJYC010000011 DAMJYC010000000
VERSION     DAMJYC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.011"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            114..476
                     /locus_tag="PC472_000777"
     CDS             114..476
                     /locus_tag="PC472_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726128.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            642..1160
                     /locus_tag="PC472_000778"
     CDS             642..1160
                     /locus_tag="PC472_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726129.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(1224..2810)
                     /locus_tag="PC472_000779"
     CDS             complement(1224..2810)
                     /locus_tag="PC472_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726130.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(2786..3307)
                     /locus_tag="PC472_000780"
     CDS             complement(2786..3307)
                     /locus_tag="PC472_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2726131.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            complement(4009..4197)
                     /locus_tag="PC472_000781"
     CDS             complement(4009..4197)
                     /locus_tag="PC472_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726132.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            4451..4627
                     /locus_tag="PC472_000782"
     CDS             4451..4627
                     /locus_tag="PC472_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726133.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
     gene            complement(4846..5022)
                     /locus_tag="PC472_000783"
     CDS             complement(4846..5022)
                     /locus_tag="PC472_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726134.1"
                     /translation="MFKNKMDKCTHMLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(5077..5174)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5383..5772
                     /locus_tag="PC472_000784"
     CDS             5383..5772
                     /locus_tag="PC472_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2726135.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKKRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKG"
     gene            5866..6249
                     /locus_tag="PC472_000785"
     CDS             5866..6249
                     /locus_tag="PC472_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2726136.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            6928..8358
                     /locus_tag="PC472_000786"
     CDS             6928..8358
                     /locus_tag="PC472_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2726137.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(8506..8856)
                     /locus_tag="PC472_000787"
                     /pseudo
     CDS             complement(8506..8856)
                     /locus_tag="PC472_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8851..9372
                     /locus_tag="PC472_000788"
     CDS             8851..9372
                     /locus_tag="PC472_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2726138.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(9501..10295)
                     /locus_tag="PC472_000789"
     CDS             complement(9501..10295)
                     /locus_tag="PC472_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2726139.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(10519..13947)
                     /gene="uvrA"
                     /locus_tag="PC472_000790"
     CDS             complement(10519..13947)
                     /gene="uvrA"
                     /locus_tag="PC472_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2726140.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCWQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTVSIL
                     DGATYYFGKLRRKKPNGNWMLGELYAIAKDKNIDLDIPWNELPREFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSSPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPSTYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVTEAKTFFSDCSNIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            14266..16500
                     /locus_tag="PC472_000791"
     CDS             14266..16500
                     /locus_tag="PC472_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2726141.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITIGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            16756..18786
                     /locus_tag="PC472_000792"
     CDS             16756..18786
                     /locus_tag="PC472_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726142.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            18783..19235
                     /locus_tag="PC472_000793"
     CDS             18783..19235
                     /locus_tag="PC472_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726143.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            19237..20292
                     /locus_tag="PC472_000794"
     CDS             19237..20292
                     /locus_tag="PC472_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2726144.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            20359..20667
                     /locus_tag="PC472_000795"
     CDS             20359..20667
                     /locus_tag="PC472_000795"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2726145.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            20771..22036
                     /locus_tag="PC472_000796"
     CDS             20771..22036
                     /locus_tag="PC472_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2726146.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            22124..22840
                     /locus_tag="PC472_000797"
     CDS             22124..22840
                     /locus_tag="PC472_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2726147.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(23035..23328)
                     /locus_tag="PC472_000798"
     CDS             complement(23035..23328)
                     /locus_tag="PC472_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2726148.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(23334..23549)
                     /locus_tag="PC472_000799"
     CDS             complement(23334..23549)
                     /locus_tag="PC472_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726149.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(23961..24473)
                     /locus_tag="PC472_000800"
     CDS             complement(23961..24473)
                     /locus_tag="PC472_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2726150.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            24923..25399
                     /gene="purE"
                     /locus_tag="PC472_000801"
     CDS             24923..25399
                     /gene="purE"
                     /locus_tag="PC472_000801"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2726151.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            25405..26103
                     /locus_tag="PC472_000802"
     CDS             25405..26103
                     /locus_tag="PC472_000802"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2726152.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            26115..27482
                     /gene="purF"
                     /locus_tag="PC472_000803"
     CDS             26115..27482
                     /gene="purF"
                     /locus_tag="PC472_000803"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2726153.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            27476..28552
                     /locus_tag="PC472_000804"
     CDS             27476..28552
                     /locus_tag="PC472_000804"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2726154.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            28546..29139
                     /locus_tag="PC472_000805"
     CDS             28546..29139
                     /locus_tag="PC472_000805"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2726155.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKIKLQGRRVFINE"
     gene            29132..30664
                     /gene="purH"
                     /locus_tag="PC472_000806"
     CDS             29132..30664
                     /gene="purH"
                     /locus_tag="PC472_000806"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2726156.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            30675..31925
                     /gene="purD"
                     /locus_tag="PC472_000807"
     CDS             30675..31925
                     /gene="purD"
                     /locus_tag="PC472_000807"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2726157.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            31950..35756
                     /locus_tag="PC472_000808"
     CDS             31950..35756
                     /locus_tag="PC472_000808"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2726158.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTGIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            35962..36834
                     /gene="rfbD"
                     /locus_tag="PC472_000809"
     CDS             35962..36834
                     /gene="rfbD"
                     /locus_tag="PC472_000809"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2726159.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            36943..37812
                     /gene="rfbA"
                     /locus_tag="PC472_000810"
     CDS             36943..37812
                     /gene="rfbA"
                     /locus_tag="PC472_000810"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2726160.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGINITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            37861..38418
                     /gene="rfbC"
                     /locus_tag="PC472_000811"
     CDS             37861..38418
                     /gene="rfbC"
                     /locus_tag="PC472_000811"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2726161.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            38456..39439
                     /gene="rfbB"
                     /locus_tag="PC472_000812"
     CDS             38456..39439
                     /gene="rfbB"
                     /locus_tag="PC472_000812"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2726162.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVNSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKVILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            39469..40137
                     /locus_tag="PC472_000813"
     CDS             39469..40137
                     /locus_tag="PC472_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2726163.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            40146..40574
                     /locus_tag="PC472_000814"
     CDS             40146..40574
                     /locus_tag="PC472_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2726164.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            40598..40912
                     /locus_tag="PC472_000815"
     CDS             40598..40912
                     /locus_tag="PC472_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2726165.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            41064..41339
                     /gene="flgM"
                     /locus_tag="PC472_000816"
     CDS             41064..41339
                     /gene="flgM"
                     /locus_tag="PC472_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2726166.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            41344..41760
                     /locus_tag="PC472_000817"
     CDS             41344..41760
                     /locus_tag="PC472_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2726167.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKKTTTYNSYGNIRK"
     gene            41778..43088
                     /gene="flgK"
                     /locus_tag="PC472_000818"
     CDS             41778..43088
                     /gene="flgK"
                     /locus_tag="PC472_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2726168.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            43107..44039
                     /gene="flgL"
                     /locus_tag="PC472_000819"
     CDS             43107..44039
                     /gene="flgL"
                     /locus_tag="PC472_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2726169.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            44055..44447
                     /locus_tag="PC472_000820"
     CDS             44055..44447
                     /locus_tag="PC472_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2726170.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            44441..44653
                     /gene="csrA"
                     /locus_tag="PC472_000821"
     CDS             44441..44653
                     /gene="csrA"
                     /locus_tag="PC472_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2726171.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            44675..45070
                     /gene="fliS"
                     /locus_tag="PC472_000822"
     CDS             44675..45070
                     /gene="fliS"
                     /locus_tag="PC472_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2726172.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            45070..45426
                     /gene="fliS"
                     /locus_tag="PC472_000823"
     CDS             45070..45426
                     /gene="fliS"
                     /locus_tag="PC472_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2726173.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            45447..46970
                     /gene="fliD"
                     /locus_tag="PC472_000824"
     CDS             45447..46970
                     /gene="fliD"
                     /locus_tag="PC472_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2726174.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            46973..47308
                     /gene="fliT"
                     /locus_tag="PC472_000825"
     CDS             46973..47308
                     /gene="fliT"
                     /locus_tag="PC472_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2726175.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            47410..48282
                     /locus_tag="PC472_000826"
     CDS             47410..48282
                     /locus_tag="PC472_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2726176.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            48375..50516
                     /locus_tag="PC472_000827"
     CDS             48375..50516
                     /locus_tag="PC472_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2726177.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            50540..52417
                     /locus_tag="PC472_000828"
     CDS             50540..52417
                     /locus_tag="PC472_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2726178.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            52410..54524
                     /locus_tag="PC472_000829"
     CDS             52410..54524
                     /locus_tag="PC472_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2726179.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            54533..55528
                     /locus_tag="PC472_000830"
     CDS             54533..55528
                     /locus_tag="PC472_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2726180.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            55620..56852
                     /locus_tag="PC472_000831"
     CDS             55620..56852
                     /locus_tag="PC472_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2726181.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            56872..57816
                     /locus_tag="PC472_000832"
     CDS             56872..57816
                     /locus_tag="PC472_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2726182.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            57910..58317
                     /locus_tag="PC472_000833"
     CDS             57910..58317
                     /locus_tag="PC472_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2726183.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            58404..>59486
                     /locus_tag="PC472_000834"
     CDS             58404..>59486
                     /locus_tag="PC472_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2726184.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIIN"
BASE COUNT        23417 a         6366 c        10155 g        19548 t
ORIGIN      
        1 ctttataaag cattggattt tattaataga tagataaaag ttatcaacta gaagtggttg
       61 tttgttgtga taactctatt attgtaatat aatgtaaata tattattgtg ataatggagg
      121 atttatgtat ggatgcattg gtttatgaga gatttgtaag agcagctttt agtattaaac
      181 ttaataactt gattaataga agcgaagatt taggtggatt ggctgaagct gatatcttta
      241 gagcagcaaa taatttacat gaattaaatg agataaaaat aggtactggg tatgctattg
      301 caatatttaa taatgagata ttagagtctt gtaatgtttc tgataatgat agcaatagaa
      361 tgattgaact atttgataga agtttaattg caacttctag ggaggaaata ttggatatta
      421 taagagaata tgaaacttat agaggacgat atcttacttt taattggaag agatagaatg
      481 tttaagttag gaagtagtaa ttatagttag ttattacttt ctttcacata tttttattat
      541 ttcaataaga ttatttccaa ataataattt attcaatgat aataagtaga aaataaaatt
      601 ttgaatcaat ataaaattta atgaaatgag gtagtaaaat catggataaa aaaatagaca
      661 tagcgaagtt tatagtaaat agaattgatt actatattga aaaatcagat aataaagcta
      721 gttttttact attattaaat agtgcaatta taggtttttt attttcaggg aaggaaaaaa
      781 taatgaatca cttaagtata agtaatatca gatgtttaga gatgctattt tacattgtta
      841 tattatgtat atttgttatt tcaatttatt tttcgattat ggttttaaag ccaaggaatt
      901 ctaaaataga aaatgaatat aaatctattt tttattataa agagatagca tctttaaata
      961 atgaacagta caaagaagca tttgaaaatg catttaaaga cgaagaaaac ttgattaatg
     1021 atgcacttgt tcaaataaaa gaattatctt taatctgtga taagaaaatg tataatgtaa
     1081 aaaagtcagt agaagctttt attttaggtg gtattatatt gtttatatat atacttgtag
     1141 tagtttttaa tgctatttag aaaatgtttt atttggttag tattcctcta tgaagataga
     1201 atcaaatcat agaagaatac tatttacata tttgtagatt cttgtaattg attttcattt
     1261 aattttttaa atgctagttt taaagtttct ttaaaatcag gtactgtgtt ttcattaaaa
     1321 tcttcaagtt catcctctcc tatccatgca agttcattat gttttgatgt gggttcaaat
     1381 ttagaaatat cataatcatt tattatttct gcgagagtta taatgccttt gtgtaaacca
     1441 tcactttttt taacttgata aattgctaga ggtataggtt ggcaatcatc ttttcttgtg
     1501 caatcagtaa taggttcaat gtttatatta aaatcttttt catattcatc tttaatagta
     1561 tttataattg aagtttctag acttgcctta gcacaaccaa attcccattt actcgcatta
     1621 ttatttctat tatcacttct tttcgcaatt aatatttttg aagttgattt attataacaa
     1681 accgcaacac agtgtaattc cattaaatct acttttatat agtctttatc tattgtattt
     1741 ttatcataac tggggtttgt tttagaaatt acgtcaccta ttttttcaat cttatcacta
     1801 aggtttctgt ctataagtat tttatccaaa gccttgtcta cagaattaaa cataaaagaa
     1861 tcaattagta atttactttc tcctttttta ttaaaaaaat attctcgaac caattcattt
     1921 tcttctattt catcaaaaga aaaactatca tcaaattcta tatcattatt ttttccttta
     1981 ttatgatacc aaatgatagg gtatagttta tctttccaga taccctttaa ttttttatat
     2041 gtaattatat gtaatttaga taaaatatca gtttttcttg ataatatata agctagttca
     2101 aaactaagaa caagtctttc tgggcatgtt tgctttgata ttctgaaacc agcatcaata
     2161 tcatttccta agaactcaaa tattttataa ttattggata aatcgtacat agcagaaata
     2221 ttttcatatt gttcattatt ttctaaagca ttaaaattag gatttcttga tactatagca
     2281 atccaggccg ctccttttaa tgaaataata ttttgaagtt tcattaagta tttttcagat
     2341 tcagaaaatc cttctagtgt agagaatata ttaccacttt ttatatcttt agcagtgctt
     2401 gttaaaatat caaagattat atctatgtat ttataaattt catcataatt ttttagtacg
     2461 ataatgaaaa ttacctcatc accaagaact ctccaaagtt gtgctttaag gttttcataa
     2521 actctatatt gtaacttacg aattatatgt gataatacct tagcccatcc gctataattt
     2581 atatttttat ataatgatga attagctata tcaaaagaaa agaacaaaat cacttctgga
     2641 tgattaggtt ctttttcttt ttcataaata tcagtattaa tattttcttg aagtttttcc
     2701 ttatactttt taagattaaa agcgttttcg tttttagctg aattatgaac attatttaca
     2761 ttttgagttt tatattcttt atcatctacc attgtaaata tcctaaccat ttccctctat
     2821 tagatgctgc ataagaggaa acattaaagt attctgcaat ttttgaagtg tcgaccttat
     2881 tacctactgt gttttcatcc ataattcttt tatattcatg tttaggcatc aacaaggctg
     2941 ctgcaaattc attggattga tactccttat tggtattgcc acttctaaaa tactgatttc
     3001 catcttgact attccatagt tcatcatcaa tgccataacc catgtgtaaa aataaatgtc
     3061 caagttcatg agcaatggta aaattctttc tggtatctgg ttgataagga gataccacta
     3121 tttcaaatga atcatcaact tttctaataa atccatcaga gtatccactt aaagaactat
     3181 cttctattac cttgcctcct aaagcatcaa caacttgatt tatatttact attggaattt
     3241 ggattttgta tgtttggata atatcttggg ttatatcatt aattagctct ctgattttga
     3301 aatccataaa atcacatcct tatataatat tttatttaaa taattattta ttataatatt
     3361 cattatatca aaaactattt tttttggtac atttaatatt aattattttc attaaccttt
     3421 tgtattatac aatataatta taccatataa aaactgtttt ttgtcgaatg tatgtttgaa
     3481 aaaataaaaa ttagtaaatt atcttaaaaa taattagata tactttggaa gcaataagaa
     3541 tattaacaat aagataaatc gttgaaatac caacatgtgt taaataataa caataaacaa
     3601 ataataaaat tttagatttc agaatatgaa agaaccaaaa aacgattaac tagtaatctt
     3661 aattaaattc atttagttga tgttaaagta tgaaaactat agtagaagtt gtaataataa
     3721 aacgaacgaa aattatcata ctaaaacata atacatgcta taattgtatt agataaatgc
     3781 ttgaatatat accaaaagta ctctttttat aagagtgctt atttttttga aattcatcaa
     3841 catataaact atcaagaaca ttactcaaca caccttaaaa ttgatttaaa ttctttttca
     3901 tacacaaagt tatatgatat aataaaaaag taaatatgta accccaatac atctttacta
     3961 agtcaaacat tattatatag agcattcttc attatggaga gtgctttttc atttctttga
     4021 ataaccatgt tgattattta taatattttc atttaattta tctttatcta tcaaatttaa
     4081 tgcacaatta atacaaatgt tagttttcat atgacttttc ttatgaaaag taatatactt
     4141 attatcatct ttatttattc ctttattaca gtaatcacat aatatagtct tagtcatatt
     4201 tttattcctt tcattttata ttttccataa ttaagtttaa catatttagt atatacctat
     4261 gtatatatat ttaaaacaat gttttttatt gcaatataga atatattgta taaataaaaa
     4321 aatggagaat agatatttga taattgtaaa attatgttat aattaaaatg caagaatata
     4381 tcttgcaaga ggctacaatc taagggcgaa agttttcgga aatcaccttc gaacgaaagg
     4441 gggtgagtat atgaaaacac ttatgtttat acttgttata gcgtattaca ttactattgt
     4501 aataaaatct attacaacaa tagtaaagtc tataacaaga ttattagatt ctataagtag
     4561 attaaaagaa tctttacata aactaagaaa tcgtcctgac ggcaatcaag acgatttcaa
     4621 tgaataaatc attacttaaa ttattttgaa tttgaaactt cgctctaggc taatagattg
     4681 tagtttcttt ttttgttttt gatacgaatt tacatcttta ttatatcgca ttttaagaaa
     4741 aaataaaact atgaatatta taaatctatt ttaattaatt ttttatcata tcattattta
     4801 aaagtagata aaactaaaaa gggttctcta gaaatcaatg tctatctaat tatacttact
     4861 taccaatacc atcacttggt gtaaacaaga ctctactaca ttttctccgt actctaatat
     4921 aaaatcatct aactgtgtat ctaaaaaatt acaataatca tacgaactgc taatataagc
     4981 agtcaacatg tgtgtacatt tatccatttt atttttaaac atataaatta ttatccccct
     5041 aaaaaacttt cataaaaaca aaaagaacaa tatctttttg gcaactggct gacataactc
     5101 ataagatatt tatataagtc ttagtcccta tagctttgcg tcactaaatt tctctagttt
     5161 tgccgattta gttttattct acaactaaaa taatacaata gaattagtta ttattcaaca
     5221 aaattgtttg aaaatttaat aattttatag atatgaaagt tattttcttc taagaaaaca
     5281 gactaaatgc gtagaatatt tggtaaaact tacctgtatc aattgaaatc agaaggtttt
     5341 attattgatt tagattagaa caatttgagg gagtgacata aaatgaaaca acaagaagta
     5401 gttaataaac tacgcagggg agaattgatg ttgatggaat acttgtggag gaaaaattct
     5461 attttatcta aaaaagaaat aatagaagca atgaaaaaaa gatataagtg gaggaaaagt
     5521 actacagaaa ttctattaaa aagattagta aaaatgaaaa cattaaaaaa gaagaggata
     5581 ggttttcagt ttagttatga agtgctagta actaaaaaag aatatttaaa tgcaataaaa
     5641 gaagatagaa aggtaactaa atatgatagt ttttttatac gaatgtttac cactatgcat
     5701 aaaaaagaaa agatgacaga agaacaaata aaaaaatata tagaaaatat ggaaaaaatt
     5761 ggaaaagggt aatttatata tatattaatt atttttaaaa taaatttgtt ataatattta
     5821 aatatacata aacatacata aatttacaaa aagggggtgt ggcttatggg attcaagaaa
     5881 ttgccagatt ctgagttaaa agtaatgaag tttatctggg gattagatgg aaataaggta
     5941 gcatcaggag aagttgtgga agcaatgaaa caaatttatg actggaataa aaatacgaca
     6001 ttaacaatac tgtctaagct tgcaaagaaa aactatttgt attctcaaaa aacaagcaag
     6061 tgtacatatt atactatcgc tgtaagagaa aaagattatt taaaagttga aacaaaaaaa
     6121 ttctttagtt tttttcataa taattcttta aaaagttttt tcactgcttt aaatgatgaa
     6181 gaaaatttaa gtgatgataa attagatatg ttggaggaat gggtaaaaaa ttgggaagaa
     6241 gatgaataaa atgaatgtga tactaaaatg atactaatat gttttatagc gtgttaaaaa
     6301 atatagaaaa tatagaaata atagaagaaa attaatgtaa tagataaagt tttatcagat
     6361 taaaaagaaa atcaatagaa tgggaataaa atatagaatg ccaaaaatcc acataaatac
     6421 aggatttgag gtctctctta cttctatgtt atactggttt gatactatca atatttgcca
     6481 gtatcttatg gattaataag caaaaaacat cctgctgaat gataaattca gcggggtgtt
     6541 tttattttgt tttagaactc ctttgctctg aaactcgaag tatttaacac tgatgatgtt
     6601 gttatctttt agttcctttt gtgtcatgct ctgaaagaca aattgataca ataaaaaagt
     6661 aatttcttaa tcattgatgg agatatgtat ttatgttact cttgatggta atttgagtaa
     6721 tattgttcat agctttttat attcacttaa ttagaaaacc atttggtata atcataaata
     6781 aacttgatga tataatagaa aagatttata attattagaa gtatataaaa gtattttatg
     6841 tctatgaaat gtatgtctat aaaacatata gatgactttt gtatattaat gtatgaatat
     6901 tgatattata gaagaattga gttgtcaatg atactaaatc aaatatttat agaagctgga
     6961 gggattatga gaaataaaat aattatagga attacttttt cacttgtatt actaatgtct
     7021 ggatgtgctt caaaacaaac cgcagaaata aacgtattag caactactga tttacatgga
     7081 gttatacctt atgagttaac ttcttatgta aaagaagaaa gaaaaaaaga taaaaatcta
     7141 actttagtag atgcaggaga tttttttgat agtggagaag tgtttggttc tagcatggat
     7201 agttatttta gtgctagata taaagatggt gataataaca ctgaaagtta tattgagacg
     7261 cctatttcaa aagatatgaa agaggtaggt tatgatgttg cagttcttgg gaatcatgaa
     7321 tttatatcca acaataaatt ttatctagat aatatgatat ctgattttga gaagcaaaaa
     7381 ataaatattt tatcggcaaa tacgtataaa aaaaatggag atagttatac aaaaccatac
     7441 ataataaaaa atattaatac acctgaagga aacgtaaaac taggaatttt agggcttact
     7501 ataaaagaag ttggtgagag gaaacagtgg aaagatggaa aacttgttga ctctaagtct
     7561 ttagaactaa aagaccaaga gggttacaat ggtgaattgt atatgaatga tttagtagaa
     7621 gatgctaaaa agtgggttgg tataatggaa aaagagaagc ctgacattat agttgctgtg
     7681 gcacatacag gtgagaaacc taagaaacct aaaaacccag gaaatagaat acaagactta
     7741 gctcaacaag ttgatggaat tgatgctata gttgcaggtc ataatcatgt tcaaattgag
     7801 caacatgatt ataaaaataa atcaggagaa aatgttatag ttactgagcc aggaaagcat
     7861 ggggaatgta tttctaagat taattttaaa ttagaaaaaa acaatgataa atggaatata
     7921 gttgacaaat ctagtaaatt aattaaattt gaaaaaaatc aagaaagtga taaatttgga
     7981 gaatttattt cttcaattaa taaagtaagt aaagataaga aagaaataag attatcagaa
     8041 gtaaagactc ctgaatggga taaaatatat gtttttaaat ctggaacatc tgcagatgat
     8101 atatataaaa caataggata taaatggaga aatatatcat ctccaaaggg tgatgacatg
     8161 gtgcagattg ttgtaatgaa taataataag cctgttagct atgtatatgg tgaagctgaa
     8221 gttatgccaa ttaatataaa atgtgatgaa tcagagtata aagataatgt tgtaactatt
     8281 tatcctaata aaaatgataa atttaaaatt gaaaaaggta aacaagaata tagtattaat
     8341 ttaaaacata tacaatagaa acttttatct tatttaagtt tagattgtac ttaaaataaa
     8401 gtgtgtatta ttttaaaaat tgatgataat taaaatcaca acattaagga atagtacaca
     8461 ctttttatgc taaaaaatag aatgttaaaa aattaaacct ttaaattaaa gtacattgct
     8521 tatattatcc aatattattt tcaaaggttg tgtaatattt ttatccttat gtaatattgc
     8581 taacatttta ttttccatat ctatcccatc taattcaatc aatcgcaatt ttttatgtaa
     8641 gagtgaatct aataataaat tttctggcaa aatggttata cctaaccctg cttgtgcagc
     8701 tttaataagt gcaaaagaat taacactttc ccaaacagga taagcttttt ggtttgaaag
     8761 agataataca ctgtccaaag tattgcgaat ggcacttcct tgctcacgta acaataacgg
     8821 aaacgtgcat aactgtcgta gagataaggc ttgtttagta ttggtggtgg ttatgcaatc
     8881 atacttttaa tccaagagaa agtagtaaat caaactggat ggataagtga gaaaatgttt
     8941 acagatatta taactatttc acaaatgaca ccagggccac tggctgttaa tacttctact
     9001 tttgttggac ttcaaattgg agggttttgg ggagcagttg cagccacttt aggttgtgta
     9061 ttatgtggtg tagtaatatc tctattttta tataattttt ttcaaaagca tcagaactct
     9121 acttatatat ttgaaatatt aaatggtcta aaatcagctt cattaggttt aattatctca
     9181 gctgcggcta caattataat ccttgcatta tttgggacga ataaagtgta cttcaattct
     9241 atatttgaga tgctaaactg gactgcacta ataatctttt ttgttacact actgatagta
     9301 cgtaagtgga aaattaatcc tattgtgatt ataataataa gtggtatact gggaatattt
     9361 ttttatttat aagtttttgg taagtagagg atatctattt ttttatatct taaatccaaa
     9421 tagctaataa agtaaggcta tttggatttt taataaattc tgaaaagata atcagaaacc
     9481 catataacat ttaaattata tcactggttt tctacaaacc aatcataagc ttcttggcaa
     9541 aatttctcac tatctatata gtaactatta tgaacattat taatttcaac aagtttcttt
     9601 ttacatttta gtgaattata atagctatca aaatattggg ttttaaaata tctatcatga
     9661 agtgttttaa agacaagagt aggtatgttg actccatctt ttatcttact atcttttgca
     9721 ataaagcact ctaacattgt attaattatc tttgtactat ttttccagat atgaggattt
     9781 ttcttttcaa atattttata aaattcatta tcatagactt cattataacc tttatattta
     9841 ggtggtttta ctgttatgtg aaaattagga agaaacttaa agaaaaagtt taataattta
     9901 acagtgagat aggtatagcc tactcctgtt gtgtttttat aatcaacaca agcaaactga
     9961 gcaaaacttt ttaacttatt gtctgaacat gcataatatt gtgcaaacat accaccaatt
    10021 cccgtattcc catatagatg tatgttggaa gaataatttt tttctatgta tgtaatgaca
    10081 gaatctaagt cttttacaat tgaattgcga gaaaaatctt tttcatatcc atcacttttc
    10141 cctgttcctg aaaaatcaaa agcaaatata tttacattat gtttggcaag tggttcatat
    10201 gctttttgca tctcaagcgt agtgacaata ggagtatgtg taacaacaat attcatggat
    10261 gattttctgg taataaatga gttacaaaaa atcattgtat ctccatttgg tatttttata
    10321 tgtttccaac tcatttgatt ttcctttctc tattataaaa tataataatt tttatgttct
    10381 gttttatata ttattcatat tttatcacag ataatatact aaagttaatt tttttattgt
    10441 acacctatta taataattct tctataatat aagacattct ataattttat atttaagttc
    10501 atgtgtattt aattgttttt atataaaatg tccagtaata ctatttggat ttatttttat
    10561 ttcatcagga gttccagaag caacaatttc ccctccattt attccactgt ctggacctat
    10621 atcgataatc caatcagaag cacgtataac ctctgtattg tgttcaataa ttaaaagtga
    10681 atgctgttca tttacaagtt ttctaaaagt aattaacaaa ttttctatat ctttaaaatg
    10741 aagtccagta gtaggctcat ccaatatgta cagcattcgt tttttagttt ttgtccctaa
    10801 ttcttttgct aattttatcc tttgagattc tcctccactt aatcctaatg tattttgtcc
    10861 aagctttaga tatccaagtc ccacttcatc taataaggag agttttctat atatattaga
    10921 gcaatcgcta aaaaatgttt ttgcttctgt tacttccatg tcaagtatat ctgcaattgt
    10981 ctttcccttg tattgtatag acagtatttc tttttgatat ctcttacctt gacattcact
    11041 acaagttacc cattggtctg ccatatattg cagaataatt tttgttttac ctaatccttg
    11101 acagtttggg cattgcccct ttttgctatt aaagctaaaa tagctttcat ccaataattt
    11161 ttgttttgct tgtggagttt cagcaaatag agaacgaatt tcatcaaaaa tattgatata
    11221 tgtagaaggt gtagaacggc tagattttcc aataggtgtt tggtccatta agataaaatc
    11281 atcaatagca tcaaatccag taaattcagt ataattttta tctgggctag atttttgttt
    11341 caatttttct tctagagcag gaagtaaact gtgaaataca agagagctct taccagaacc
    11401 actaacacct gtaatagagc acatacagtt taagggaatt tctacatcaa tattttttaa
    11461 gttatttgca tggcatcctt ttaagcttaa ccatttactt ggtatagttt tattttgtga
    11521 acctgtttta ttgtatgtac ttaaatattt acctgttata gagtttggat tttttataat
    11581 ttcttgggtt gtaccttctg caataatgaa cccccctttt gtaccagcat ttggacctac
    11641 atcaataata taatcagcag acagtattgt atctttatcg tgttccacaa ctataacagt
    11701 attaccctta tctcttagct cttctatcat tttgatgatt aaattatggt ctcttgggtg
    11761 taaaccaata gatggttcat ctaaaatgta ggttaagcct actaattcac ttcctagctg
    11821 acttgataat cgtactctct gcagttctcc tccagataaa gttggagctg ttctatctgt
    11881 tgatatatag ctaagaccta cctttaatag ataactaact cgaattttta attctgttaa
    11941 gatatcattt accaaagaga gctcattttt ttgaagttga tttggtaatt cacacaacca
    12001 attccataac tgttctatcg tcatttttgt tagttcaggg aagcgataac ctttgattgt
    12061 agtgaatcta gcctcaatgc acagcaattc gccaccacat gtattgcaag gaattttgtt
    12121 catatattga tgcaaggtat tattttcaga actagattct ctaaaaagtc tctgaatgtg
    12181 gttgattgct cctgaagcag gtcttctaat ctttgattct cgtccctttg attcaaagct
    12241 aaattcatat atcttatcgt ctgtaccata taagattgca tctataaatt ctcttggtaa
    12301 ttcattccaa ggtatatcta ggtctatatt tttatctttt gctattgcat ataattcacc
    12361 taacatccaa ttaccatttg gctttttcct cctcaatttt ccaaaatagt aagtagctcc
    12421 atctaatatt gaaacagttg ggtcttttac aattaattga ggattaactt gataggtaaa
    12481 acctaatcca ttacaatcgt gacaagctcc agtatgggag ttagcattga aggttgaagt
    12541 atttatctta gaaaaaatag tttgacaatg tggacaataa ttttttgtat tattcttttt
    12601 ttctaatgga attttgcaat atggacagta actttttccg attgatacaa ataacattct
    12661 taaataatcc caaatgcttg taagagtacc aacagtagag cgaggatttt ggcttccttt
    12721 tttctgttcg atagctatag taggggttaa tccctgaagt aaatcaaatt ccggtttttg
    12781 aagctgttct gaaagttttg ctccattacc tagattttca aaataacgtc tttttccttc
    12841 ttcataaatc gtatcgaaag ctaaagaaga ttttccacta ccacttacac ctgtaataac
    12901 agttatttga ttttttggaa ttgtaacctc aacatccttt aaattgtttt gccttcctcc
    12961 ttttaaatag atagagcttt tattatgaca aagtgaattt ttaattgatt tatttattga
    13021 ttttgtaaat ttgaatttct tttctagttc aagaggattg cctaattctt ttatttccga
    13081 aatacctaat atttttaact gagaaaagat tgatttcaaa taatcttcag gcaatttttc
    13141 tattaacaaa ttaaggtctt ctatttttat atttccgctt gataatattt ctgccatttc
    13201 atctaataaa tttttttctg ctcttgttaa ttgattgtat tttttggaac ttaattctgt
    13261 ccataaatcc catggaagca attctaaacc aatcatatta ttaaagtcat gtagaagatt
    13321 accctttaca gaatgccaac ctttccaatt tttattatat ccaaagtcat ctggatgagc
    13381 atgtccttgc caacatctta tccaggcttc tgaaccagtt aaaaaaccat ctgtttttcc
    13441 gacatcatga gtgtcaaaat taatttgaaa ataatttttt tgtatatcat ctatttgtgc
    13501 atctattctt atccatctct ttgttagagt atcatagtat tctacaagcc agtggtcttc
    13561 ataagtaagt tcactttcaa aataattagc aaaacctgct ctcattctag cctcaaaacc
    13621 tcgatatctt aggagcgata caagaagtaa agaataatct ctgcacattc caacaagcct
    13681 ttgttttggg tttcttggaa tagttagtgg gttagaattt atttcaaata ttctttttaa
    13741 catttgagga attgtacgaa gcaattcctc atcagattgt tgtttattaa aggatatacc
    13801 atataatttt ccttggtctc catgaattac taggctttga accactttta ataattcaat
    13861 tggagtcttt ggtaaatctt gatatttatt ttcaaatttt ttaggatttg tgatgatgct
    13921 ttgattttta tagttattta ttttcattta tatttcacct ctctaaatgg atgatatacc
    13981 ctgacatggt gtcatatgca aggacaattt agattttttg gccaaaattt aaaaataaat
    14041 aaaaaatgat acagtgataa attgtaatac aaatatagtt tcaaattgca tataatataa
    14101 ttatttaata aaaccattat ctatggaatg ttaatgaaat gttaagatag cattcttgaa
    14161 tattaagaaa tagaatacta taatatagtg tgaaggattc ttagtaaaaa aatttgtaag
    14221 tgttaatata cattaaaaaa taggtttata atttggagga aaaaaatgaa attatcaagg
    14281 tataagaaaa atagtttagt tctgtctata attttatgtt cacttattat ttcaatcctt
    14341 gtaattactg tttttaatac cgtaaaacta cacaatatat ctaataaaca atctaaaaac
    14401 tatttaaacg atgtatctac acagattgta atgaatgttg attctaaaat caaatttatt
    14461 ttatccgact taagaataat ggcagatttt attaagcaat atgaaggtga tagtcgatat
    14521 gagtatttat ctaaaagaaa gactagttat aagtattatg atgttggaat aatagattta
    14581 gaaggtaaag ctgagttttt aagtggaaaa aagtttgact taaagaatac tgcttcctat
    14641 aaaaaaggaa ttgaaggtaa agaatactca gaagtaatag agtcaatggg ttttgttctt
    14701 tatagtgtac ctatatttaa tgataatcat gaagtatcta gtattttaat aggtgtatcc
    14761 aataaagaaa gtatgaatga gattcttaat atagataact ttaatggaga aggaactatt
    14821 gaaattatta atagtaaggg aaaacctctt ttcataggaa aaaatactga gttaataaaa
    14881 gaccttagcc ataaatataa ggattctgca gatgaacctt gggcacaaaa gatgcttaat
    14941 gattttaaaa atgataaatc agggaatatt acaataacct catcaaaagg agtacaatgc
    15001 ttattaactt atcatccaat tacaataggt ttaaatgatt ggcactttct tctcatagta
    15061 cctgaagatg ctgtacttgg tgaacttaat aagttaaatt cttttacaat aacaatgaca
    15121 tttataatta catgtattat tggaattata acattaattt tatatattat aagaagaaaa
    15181 tatgtaaatc aaatagaaaa tattgcatat ttagatagta taacaaatgg cataaattca
    15241 actaagttta gtatgctagt caaaccttta atttcagaat cacctgatag tacttatatg
    15301 atgatagcaa tgaatataaa agattttaag ctaataaatg attgtttcgg tagtgaaaag
    15361 ggaaatatga cactaaaaca tctttacaat attttgtata aaaacattga tgaaaatgaa
    15421 gaatttgtgt gccgtcatga tgcagacctt ttctatttgc ttattaagaa tagaccagtg
    15481 ttagaagccc tggagttatt atataaaata gaagctgaca caaattattt taataaaaat
    15541 agggaaaatc catacttttt aagactatca gtaggtattt acacaattga aaatcataat
    15601 gaagatttaa ttacaataca agaccatgca aatacagcaa gaaaaagctt taataaaact
    15661 catcaaagtg atttttcttt ttatagtgat atagaaagaa aacgattaat atctgaaaag
    15721 gaaatttcaa atttaatgga aaaagccctt gaaaataaag aattttttat gtgcttacaa
    15781 cctaaaattg atataaaaag tggaaaaatt agtggagcag agtctcttgt gcgttggaaa
    15841 aattcagaaa agggcataat ttatccaagt gattttattc ctcttttcga aaaaagtgga
    15901 tttatatgta agttagattt atatatacta gaagaaacat gcaaattgat ttctaagtgg
    15961 ataaaagaag gtaaagaagt gttgagaatt tctgttaatg tatcacgtca acatttgaat
    16021 gataaattgt ttttagaaaa atataagcat atatgcaata aatacaatgt accaacatgt
    16081 ctgatagatt tagaacttac cgaatctatt ttcttagaaa atccagaagc tatagatata
    16141 atagaggata ttcattctaa tggatttaaa tgttctatag acgattttgg atttgggtac
    16201 tcatcattag ggatattaaa ggattttaaa gttgatataa ttaagttaga ccgttcattc
    16261 tttgttagca aaaataatat agaccgtggt aaggtagtta ttaagtctat tattgaactt
    16321 agcaaaaggc taggtatgaa agtaacagct gaaggaattg aagaattaga acaagttgag
    16381 ttcctaaaaa atgtgggatg tgattatatt caaggttatg ttttttcaaa accattatta
    16441 atttctgact ttgaaaattt tgcatatgaa aataataaaa ttaaaaatat ccattattaa
    16501 atgtacaaga taaaaaattt aggcaagtta tataattaaa aatataactt gcctttttta
    16561 tttatatatt taataaaaat atatttgcag attttgtaaa aataattttc aaatattgta
    16621 agttgtggct aaagctttat tgtattattc tgaatataga aagatagtta ctgtcttaga
    16681 taaaaactga aacatacata ataatataaa atttgctata ttaataactc taattatttt
    16741 ggaaggaggg aaaatatgat aagtgggaga atgttactca tcatgaattt tctaaaagga
    16801 aaaaataaga caagttacaa ggatattagc aaacaactta gtatagaaga aagaaaagtt
    16861 agatatgata taaataattt aaatattgtt ttgcgttcat taaacaagtc agtaatacaa
    16921 aagatgaaca aaggtgtgtt aattattcct gatgacttcc aagtagtatg tataaataat
    16981 aacgaatatg tattttcctt aactgagcga gtatctatta tgaaaattat atcaatgttt
    17041 aaaatagata atctaaactt agaaaaatta agtaaagaat ttcaggtttc tagaagttca
    17101 attaaaaatg accttaatgt tttgtcaagt gaattagaaa gaaaccaaat aactattact
    17161 tatgataaat cttttaaaat aagtggagaa gaagacatta tatataagca aagattgaac
    17221 atattaaaat cgtattctta tctttttggt aaggaaaaat ctgatttaag tgtatttgag
    17281 aagtatttag tgaatgtaat tgaaaaaata ttcaaaggga tttatttgat tgatatatat
    17341 aattggtcta tgaatttatt attgcaaatg ggatggactt taaatgatga atcttttaca
    17401 tggtatgtgt caaacatttt cctttttaca tggtatatac attcagatga aaaacatcct
    17461 ttagaatctg cttcattaag tgagccgtgt tttgaaaata agtttgacca agaatttgaa
    17521 acaattattc aaaaaaagtt gactaaggaa caaatttttc tgttaaagag ttttgtattt
    17581 tttacaaata aatatgcaag tttaaacgag gatatggatt tgattagtac agaaattata
    17641 gttcaggact taatttacaa tatgtcagaa atattagaga ttagctttaa agaagatatg
    17701 attctttata aaggattact caatcatatt gccccattga tagagagaat caagcagaat
    17761 atacagattt atgaggaatt acttcatgta ataccagata aatatcaata tgtacttgaa
    17821 gctactaagg tttctattca aaaaaacaga gttttaagtc aaataaagaa tgaaaatgaa
    17881 acaatacttt tggcaataca tttcttagga agtatacata gaaatgagag gaataactac
    17941 aaaaatatat tgttagtttg tggatttgga tatggtgtaa ttgctatgtt aaaggatagt
    18001 ttagtaaatg attaccagat aaatatagta gagagtattc catcatataa gttacaagat
    18061 tataaagaat gggaaagtat tgacttcatt ataacaacat caaagatagt aatagacttg
    18121 cctaaaacaa ttattgagat aaatccattt ttacaagaag aggattattt gaagtttgaa
    18181 gaaaaaggga taaaaagaaa gaacgtctta actaattata tgtcaataaa aaaaaggttg
    18241 gactttttag aagaagatgc ccaaaaaaga gtattaagta ttatatgtca agaacttgga
    18301 tacagtgatg agagaatatt atttagacaa cttaatttaa gtgatttaat agggattgat
    18361 accataagga taattgatgg agatatgaaa tgggaagatg ctgtaaaatt tagttcaaac
    18421 atacttgcta attgtgattt tgtatcagca caatatgctg agaatattat agatatttta
    18481 aaaaatgtgg gtttttatgc tgtaaaagat aacgaatttg ctttactaca tggcaatgat
    18541 agttcattag tcaaagtaag ttcgataagt ttattaatat gtaaagaagc agttagtttt
    18601 ggagataaaa aggttaaaat catattttgc ctagcaagca gagataaaaa agagcaaatt
    18661 cctgcaataa ttaatctaac taaaatggtt tataagacta attttattgc tatgttagaa
    18721 agtgcaaaga caaaagaaga agcagaagtt ttaattcata aatatgagaa ggaggtcact
    18781 gtatgaataa attagttaat gtaaaatgtt catttaaaat acatgttaat tcaaaagaag
    18841 aagccattat atcgttagtt gatgtagtgt caaaagaagg atatttaata gataaaaatc
    18901 aatttttaaa agatgtttta aagagagaag aaacattgtc gacttatatt ggacatggta
    18961 taggtcttcc tcatagtcaa agtgtaggtg ttaaaaactc ttgtattaca ataggaaagc
    19021 ttgatactcc tattgaatgg acagaagaag gagaaaaagt ggatttaata tttttgatat
    19081 cagtaacaaa agacaatgaa aataatcttc atttaaagat tttatcaaaa ttagcacgtt
    19141 tattgatgca tgaaagtttt agaaatcaaa ttagatgttc agatgaacaa actgtttata
    19201 atcttataaa agaaaaaata gaggaggaag attaaaatga gaattttaaa gtttttgcaa
    19261 aaacatctta tgacagcaac atcttatatg attccatttg tggttgcagg aggaatatta
    19321 tttgctttat cagtaacttt aagtggacaa gctgctgttc ctgaaacagg ctggttggct
    19381 aaactgaatc aaattggagc tgctggcttg gctttattca ttcctatatt aggtggatat
    19441 atagcattta gtatggcaga taaaccagga ttagctccag gtatgatagg tgcatattta
    19501 gcaaaagaag taaatgcagg ttttataggt ggtatcatag caggatttat agcaggattt
    19561 gttgttttac aattaaagaa aattaaatta gctcctacaa tgagaacttt aggttctata
    19621 tttatttatc ctttagttgg tacattgatt acaggtggta taattgtatt tttaattgga
    19681 gaacctatag cttcttttat gacatggatg acaaactggt taaatggaat gtctggtgtt
    19741 tctaaaattc ctttaggtgg aatcttagga ggaatgatag cctcagatat gggtggccct
    19801 ataaataagg ttgctgctac ttttgcccaa actcaagttg acacacttcc atatttaatg
    19861 ggtggagttg gcgttgcaat ttgtatacct ccaattggtt taggattagc tacattattg
    19921 tttccaaaaa aatttagcaa ggaagaaaga gattcaggta aagcttcact attaatggga
    19981 tgtgttggaa ttacagaagg agctattcca tttgcaacat cagatccagt tagagtaata
    20041 ccttgtatta tggctgggtc gattgtagga aatattatgg cttttttatt aggatgttta
    20101 aatcatgcac cctggggagg tctgatagtg ttacctgtag ttgacaatag gttggggtat
    20161 attgcatcag tattaactgg agcagtagtt gttgcggtat taatgaaatt agtaaaaaaa
    20221 gacgtgaaag aagatgaaga aattgaagaa gatttagatg aatcaattga attaatattt
    20281 gaagaattat aaatataaat ggaaggaatt tgtataaaaa agtaaatata aaactataaa
    20341 tttaaaggag ataagaatat gaaaattgta gcagtaacat cttgtcctag tggagtagca
    20401 catacttaca tgtcggctga atcacttgaa ttatcagcca aaaaatttgg tatagaaata
    20461 aaggttgaga ctcaaggaag ctctggaata gataatgaat tgtcacttaa agatattgat
    20521 gaagcaactt gtgtaatatt gactaatgat gttgagatta gaaatatgga acgttttaaa
    20581 ggaaagaagg tagtaagaat gagtgtatct gagataatta agaaatctga tgcattaatt
    20641 aagaaaataa aagatacttt tcaataaaaa ttgatttatt ttacatataa ctcaaagatg
    20701 tttttaaaga aatttttatt aaaagcatct ttgaaaatat cttaaaaata gaataaaagg
    20761 agatatagtc atgaaaaagt taccaataaa gactgtcgtg caaagattac taaacttaca
    20821 agaagagggg aaaagtgcaa ctcttttagg aattggtcct atgtcaccaa acttgttaca
    20881 agcaagtttt gaactagcaa aagatgatga ttttccttta atgtttatcg caagtagaaa
    20941 tcaagtagat gcagatgaac taggtggcgg ttatgtaaat ggttggaatc aggaaacttt
    21001 tacaaaagat attaagaagg ttgccgacaa agttggattt gatggtttat attatttatg
    21061 tagagaccat ggtgggccat ggcaaagaga taaagagaga aatgaccatt tgcctgttga
    21121 tgaagcaatg gagttaggaa aaaaatctta tttagcggac attgaagcag gttttgacct
    21181 attgatgatt gaccctacaa aagacccatt tgaaattgga aaagtaattc cactagatgt
    21241 agtactagaa agaactgtag aattaattga gtattgtgaa aacgagagaa aaagacttaa
    21301 tctaccagac ataggttatg aggttggtac agaggaaacc aatggaggtc tgacttcaac
    21361 tgagacgtat gaaacattta taactagact aaaggttgaa ctcgataata gaggtttgcc
    21421 aatgccaaca tttatagttg gtcaaactgg tactttaact agaaagacag aacaagtagg
    21481 aacttttaat tttagaaatg cttatgattt agctcaaatg gctaagaaat atggagttgg
    21541 tttaaaagag cataatggcg attacttaga tgatgtaact ttattagaac atataccatc
    21601 tcaaattatt gctacaaatg tagctcctca atatggtact gaagaaacta gggcatattt
    21661 aaaattagca gaagttgagt gtaaattaga aaaagagggt ttagttgaga aaacttctaa
    21721 tatcagacat gttcttcttg ttaatgcaat tgaatgtgga cgttggagaa aatgggtagt
    21781 aggagaacaa aaaaatctaa ctactgaaga gatatttaag gatgaagttt tatctaatga
    21841 aatactagat attgcaggac attatacatt taataatgat gatgttaaaa aagaaataga
    21901 agtgctttat gataatttaa gtaagaataa tattgatggt caaagatttg ttgtagacca
    21961 tattaaacgt cctttgcgtg attatgcaga atgctataac ttaaaaggtg ttactacgag
    22021 gattttaaat aaataatagg acttatagtg gtgagtattg ttatactagg tatttatagt
    22081 tataattata cttaccacta ttaaatttag gaggactaat attttgatta atttaatttt
    22141 tgatgtagat gatactttat ataatcaatt aacgcctttt tacactgcat ataataaagt
    22201 gttttcttca ataaaggata tttctattga agatttatat atgagtagta gaaagtatag
    22261 tgatgaggta tttcatatga ctgaaaatgg agaaatgcca atcaaagaaa tgcatattta
    22321 taggataatg aaagcttttg aggagttggg aaattctatt acagaaaaag atgcacaaag
    22381 ttttcaagat gagtatatat atcaacaatc acaaattaca ctaataccag aagttgagag
    22441 gattttaaat ttttctaaag aaagaaatat taatctagga attataacta atggcccatc
    22501 aaaccatcaa agaatgaagt taaagcagtt aaatattgaa aactgggttg ataaaagtaa
    22561 tatttttatt tctagtgagg ttggattttc taagccagat actaatattt ttagagtagc
    22621 tgagaatgtt atgaatttag atagagaaaa tacatattat gtgggtgatt cttatagaaa
    22681 tgatgtatta ggtgctaaaa aagctggttg gaagagtata tggttgaacc atagaggaca
    22741 tgaggtagaa gagttgtttt ataaacctga ttttgtaatt ttggaacata aggatttgat
    22801 atctttattt ataaaaatat gtagttataa aaatatgtag taaaaaaatg ttaataatag
    22861 aattgtataa aataattaat aataactata taaaattata tgtttagatt gtgagcaatc
    22921 tattttatgt taaaaataga ttatttagaa gaaggggaaa tcttaaaaaa atttgaatat
    22981 ccccttttta ttatttaatt aaaatgaaat agttagaaat atattttatt tagtttagtc
    23041 aataacattt tgggattctt tataactaca tgttaactgt tgaataggtt tatcatatgg
    23101 tttaacccat cctttattaa tacttaattc tgtaagtgct gtgtgacctt ctaccatttg
    23161 agttaatgaa gctgaatata tagctcttaa ttcaggtgta ctacttgtca atgcagaatt
    23221 aagatacata ttggcagatg ctttagcaga tgaaagcata cttaacatta ttactttatc
    23281 gtctatataa gtattgttct taattagatt ttctatcata tttctcatgt tatctatacc
    23341 tcctcagaaa ttagtatttc attttcaact ataaattgtt gtatagcttt aattcttcct
    23401 tctgttgcta atatactaga ttctgattgt ctttttaggt cttcatctga tattaacata
    23461 tttattgctc tttgcataag taatccgtca gactccattt ttataattgc tgctaaagat
    23521 attttttcag tttctgaaag tattctcatt taatcatctc ctatattgta atttatgctt
    23581 taatagtatg tctttctttt caaggaaata accattacac ttttttacag tattttatat
    23641 tctctttata tcaaaattta gtcgacctaa ttaatataat tttgtaggat atttatcatt
    23701 aaatatctat aaaataaatt atttgaatat tcataaagca aaatttattt gtatgtactt
    23761 cttcaagagt caatatgagt tttttcaaaa aattaatttt tatgataatc attaagggct
    23821 atatttttct taaagttgtg attatcatta ttaatttacc ttagaggtta attaggtgtt
    23881 taatcaaagt taatgccttt agaatacaaa tgtattctaa aggcattttt actatgtatt
    23941 ttcatttata ttactaaaca ttatctattt ttatatacat ttctatgtat tttattttca
    24001 tcaaatcggt ctataaactc atttttttct tcactatatc caatagatat aagtgcataa
    24061 ggaactaatt ttaatggtaa gtctaaagtt tgtctaactt tatttaccac tttattttta
    24121 ggaaatactc ctgcccaaca agcagctaga ccttgattag taacttctaa taataaattt
    24181 tgtgtgcaag cacctaaatc ttgtatccat ttaccaggtt ttaagaattt atctctattt
    24241 ccaagcacta ctatacataa tggtgcaaat tctatatgct ttgctctatg ctgtgacttt
    24301 gagagctgtt tcagcagttc cttatcatct actataataa attcccaagg ttgagcattt
    24361 tttgatgaag gtgcttgcat agctgatttt agtatttcag ttattttatc ttcttctata
    24421 tttttatcta tatatgttct tattgaacgt ctatttttta taacttctaa catacagatt
    24481 ctcctttaaa taaaattatt tctatttata taatatcatt actttaaatt ttagctatta
    24541 cttatataaa gaataaactt tgtgttggtt ttatctaagt aaaagtaaca ttcagaacta
    24601 aaaaacaaga ctataaatcc aaagtattaa ttaaaaaaga agtaataaaa aataatatac
    24661 aagatttaaa aaagagtatt gggcttaaaa tttaattaga aaaccaattt tgagataatc
    24721 tgttttttat tttaacaaaa aaacataaat aaagttcgat acgtagcgaa tgaaaaaatt
    24781 acaagaaata ttttttttaa aaaatttcaa aaaagagttg ataatatatg tatattaata
    24841 taaaatttaa ggtgtataat tcgtataata atacgaatga tttgtagtgt aaaataactt
    24901 ataaacggag aggggaagtc taatgaaagt agcagtagtt atgggttcaa aatcagatta
    24961 tccaaaatta gaagaaggga taaaactgct tgaaaaatat ggaatagaag ttgtagcaag
    25021 agcattatca gctcatagaa caccagaaca actttcaata ttcctaaaag aaatagaaga
    25081 tgatactgat gtaataatag cagcagcagg aaaagcagca cacttaccag gtgtaatagc
    25141 ttcacaaaca ttaattcctg taataggatt acctattaaa tcatcaacta tggatggact
    25201 agactcactt ttatcaatag ttcaaatgcc aaaaggaatc ccagttgcaa cagttacaat
    25261 agacttaggt ttaaacgcag ctttattggc attacaaata atgactttaa aatacccaaa
    25321 attaaaagag gatttgaaat catatagaga agagatggca caaaaggtgt tagaagacga
    25381 caagaattta agggggtagc agaaatgtta ttatatgaag gaaaagcaaa acaagtatat
    25441 tctacagata atgaaaatga atatgttgtt tattataaag atgatgcaac agcatttaat
    25501 ggagaaaaaa aagcagaaat atcttcaaaa ggtatactaa ataataagat atcaacaata
    25561 atctttgaaa tgttaaaaga aaataatata aatactcact ttataaaaag tttatcagat
    25621 agagaaatgt tagttaaaaa agtagagatt ttaccattag aagtaatagt aagaaatatt
    25681 gctgctggtt ctatctgtaa aagagttggt cttgaagaag gtgtagtttt cgatgagcca
    25741 atttttgaaa taagctacaa aaatgatgct tatggggacc caatgctaaa tgatgattat
    25801 gcagtggcaa tgaagttagc aactagagaa gagttaaaat tcttaagaga agaaacttta
    25861 aaaataaatg agttactaaa agcatttttc ttaaaattaa acctaaagtt agttgacttt
    25921 aaaatagaat ttggtaagga ttctgaagga aatataatat tggcagatga agtatcacca
    25981 gacacttgta gactttggga tgtaaataca aatgaaaagt tagataagga cagatttaga
    26041 aaagaccttg gagaccttgt agaaggttat acagaagtac tttcaagaat gaataataaa
    26101 taggaggcaa atgtatgtgt ggagttttag ggatttactc taataaagat gtaactaaag
    26161 aactatatta ttctttatat tctatgcaac acagggggca agaaagctgt ggtttagctc
    26221 ttttagatga tggagaaatt aagtacaaaa aagatatggg attagttgga gatgttttta
    26281 aggaaaatga attatctaag ttaaaaggaa atatagggat tggacatgtt agatattcaa
    26341 cagctggagg aagtcatgtt tctaattgtc aacctttagt tggaagttgt agaaaaagac
    26401 aattagccat agctcataat ggaaatctag ttaatgcaaa ttatttaaaa gatatgctag
    26461 aagaagatgg atatatgttc caaactaatt cagatacaga agtgatttta tatatacttg
    26521 ctagatatta caaaggggat atagtagaaa gtttaaaagt gacaatggat tatataaaag
    26581 gtgcatatgc tcttgtaata atgagtcaag aagaattggt tgcagttaga gacccacatg
    26641 gattcagacc tcttgtactt ggtaaaaagg gtgatgaata tatatttgca tcagaaaatt
    26701 gtgcaataga tatacttggc ggagaagtta taagagatgt agagccagga gagataatag
    26761 tagtcaaaga tggagaacta aaatcatatt tttattcaga aaattataaa ccagttaaga
    26821 aaagttgtat atttgaacac atatactttg caagaaatga tgcaactata gacaatgtaa
    26881 atgcatatga atttagaatt aaatgtggtg agagattagc tcagaatgaa actgtaaagg
    26941 cagatatggt agttccagtt ccagattcag gttggccagg agctattggt tatgcaaatg
    27001 ctagtggatt aaaaataagt gaaggcttag ttaaaaatag atatgtagga agaacattta
    27061 taaaacctac acaagaagaa agagaaatag cagtaaaaat aaaattaaat cctctttcaa
    27121 caataataaa aggaaaatct ataattttag tagacgactc aattgtaaga ggaactacat
    27181 ctaaacaatt agtaaaatct ttaagagaag caggagcaaa agaaattcat cttagaataa
    27241 cttctcctcc agtagcttac tcttgttact atggaataga tactccaaat cgctctaaat
    27301 taattgcatc aagtaacaac gtagaagaaa tgagagaata tattggatgt gatagtttaa
    27361 aattcctaga tatagaagga atgttagatg caacagaaca taagtctact ttctgtaaag
    27421 cttgttttga tggagaatac ccagttaaga aaattgataa ggaggaacta ctttcatgtt
    27481 aacatataaa gaatcaggtg tagatataga tgaaggaaat agagcagtag accttataaa
    27541 aggtaaaata aaaggaactt atgatggaaa tgtagttgga gatttaggta acttcagtgg
    27601 attatacagt ttaaaagatt ttgtaggtat gaaagaacca gttttgcttg catctacaga
    27661 tggtgttggg actaagttaa aaatagctca gatgatggac aagcatgata ctgttggaat
    27721 agatttagtt gcaatgtgcg tgaatgattt aatttgtcaa ggagcaaaac cattattctt
    27781 ccttgactat atagctcttg ggaaattagt gcctgagcat atagaaaaaa tagttggtgg
    27841 aattgcagat ggttgcaaaa tgtctggttg tgcactaatt ggtggagaaa ctgctgaaat
    27901 gcctggaatg tatggagaag atgactatga tttagcagga ttttctgtgg gaatagcaga
    27961 caaagaaaag attgtttctg gaaacaatgt taaatcagga gatgtattgg ttggtatatc
    28021 ttcaagtgga gtacacagca atggattttc ttttataaga aagatatttt tagaaactta
    28081 caattataaa atggaacaat atgttgaaga actaggaatg acagttggag aagcactttt
    28141 aacaccaact aaaatatatg ttaaattagc tttagatgta ttagctaaac atgacataaa
    28201 agctatagct cacataactg gtggagggct tatagaaaat ataacaagag ttatacctaa
    28261 agggttagga ctagatatca ataaaaaatc ttgggagaag cctcctatat ttaaaatgat
    28321 agaaggtttt aatgctgttg atgagagaga acttcataag agttttaaca tgggtattgg
    28381 gctagtttta atagtagata aagaaaatgc tgatgatgtt gtaaacttta taaataatag
    28441 agaaaatgac aatgctgatt atgtagataa aaaatatagt gaattattag aagataaagc
    28501 ttatataatt ggtgaagtag tagatagtca tgaaggtgta gaattatgct aaatatagga
    28561 gttttaatat ctggtggggg aacaaattta caagcagtta tagatggtac tgagtctgga
    28621 gaaataaaag gtcaggttaa agtagttatc tcaagtaagc aaggtgctta tgggctagag
    28681 agagctaaaa atcataacat aaaagcaatt tgtgaaactg atgaagacaa gataatagaa
    28741 attcttaagg aaaataaaat agatttagta gttttggctg gttatttaaa aataatcagc
    28801 ccaaagctgg ttaatgagtt tagaaacaaa atgataaaca tacatccatc attaattcct
    28861 tcattttgtg gagctggatt ctatggagaa aaagttcatc aaggtgttat agattacgga
    28921 gctaaagtaa ctggtgctac agttcatttt gttgatgaag gtgctgatac tggaccaatc
    28981 ataatgcaag atgtagttaa ggtaaatcaa gatgatgatg caaagacact tgcaaaaaga
    29041 gtgttagaag ttgaacatag gattttaaaa gaaagtataa gtcttttttg tgaaaataaa
    29101 ataaaacttc aaggtaggag agtgtttata aatgagtaag agagctttaa taagtgtaac
    29161 tgacaagact ggtgtagtgg aatttgctaa agagttaaat aaattagatt atgaaattat
    29221 atctacagga aatacattta aaacattaaa agaaaatggt gttaatgtaa tgcaagttga
    29281 agatgttaca aattttccag aaatattaga tggaagagta aaaacattaa atccatatat
    29341 acatggtggt atactttata aaagagataa agaatctcat gtagaaactg taaatgaaca
    29401 caaaatacat tcaatagatt tagtagctgt aaatctatat gattttgaag gaactttaaa
    29461 agctggaaaa agtcatgatg aaataattga aaatatagat attggtggac catctatgat
    29521 acgttctgct gccaaaaact ataaggatgt aatagtagta gtagatataa aagattatga
    29581 ttctataata gaaaaattaa aaacagatac aatgacatta gaagacagaa aaaaattatc
    29641 atataaagct ttctcaacta caggaagata tgatgcgtta atatctagtt attttgctgg
    29701 agaagttgga gatacgtatc ctgacatatt aaatttaact ttccaaaaag aacaaacttt
    29761 aagatatgga gaaaatccac atcaaaatgg tttcttatat tcacagtcaa atgcaaaaaa
    29821 tccaatctta aattatgagc aattaggcgg taaagaactt tcttttaaca acttaaatga
    29881 tttacatgga tgtttagaag taatgagaga atttaaagat agtgaagaag tagtttcagt
    29941 agctataaaa catgctaatt catgtggggt agggttagga aaagatgctt ttgaagcata
    30001 tacaaaatgt tatgaagcgg ataaggtttc aatatttgga ggaatagtag gaataacttc
    30061 tactatagat aaagctacag cagagaaatt aaatgaaata ttcttagaaa tagtagtagc
    30121 atatgatttt gaaccagaag ctttagagat acttaaacaa aagaaaaatc taagaatatt
    30181 aaaattagct aaaatagaaa atagtttaca accatatgaa atgaaatatt tagatggaaa
    30241 attattgata caagatagaa acaatatatt agcagaaaaa agtgaaaatg taacaaagga
    30301 aaaaccaaca gatgcacaac ttaaggacat ggaatttggt atgagagtag taaaaaatat
    30361 gaaatcaaat gcaattgcaa tagtaaaaaa tggacaaact ctagctttag gttgtggaca
    30421 aacatcaaga atttgggcat taaaaaatgc tcttgaaaat aacaaagata aagattttac
    30481 aggagctgtt ttagcgtcag acgcattttt cccatttgat gattgtgtaa cattagctca
    30541 tgaatatgga atatcagcag tagttcaacc aggtggctct ataaaagata aagattcaat
    30601 agaagcttgt gacaagtatg atatggttat ggtatttaca ggaataagac acttcaaaca
    30661 ctaaggagga tattatgaag atattggttg taggcggtgg cggaagagag catgctatat
    30721 gctggaaact aagcaaagaa aaaaatgtag aaaaaattta ttgtgcacca ggtaatgcag
    30781 gaattgcaaa cgtagcagaa tgtgtaaata taggtgatac taatatagaa gaacttttaa
    30841 aatttgccaa agaaaatgaa ataggtctga caatagtagg accagaagta ccactagtta
    30901 tgggaatagt agatgagttt gaaaaagaag gattaagagt atttggacct aataaaaaat
    30961 gtgctcaact tgaaggaagt aaagcttttt caaaagaatt tatgataaaa cataacatac
    31021 ctactgcaaa atataaagaa tatacaaact tagaagaagc tatatcagaa atagatagtt
    31081 ttggatatcc agtagtaata aaagcggacg gattagctgc tggaaaaggt gttgtaatac
    31141 ctgaaaatag agaagatgct atagcaactt taaaagaaat gatgtcagat aagaagtttg
    31201 gagctgctgg agataaaata gtaattgaag agtttttaaa aggaatagaa acttcaattc
    31261 tagcatttgt agataatgat acaatagttc ctatggcaag tgcaaaagac cataaaaaag
    31321 taaacaacta tgagcaaggt ccaaacactg ggggaatggg aactttttca cctagtgaaa
    31381 tatatacaga agaattagca aataaagtaa aagagacagt attagaaaaa actttagaag
    31441 gcttcaaaaa agatggactt aattttaaag gtattttatt tgttggtctt atgataactg
    31501 aagatggaga aaaagtactt gaatataatg taagatttgg agaccctgag acacaatctg
    31561 ttttatttag attagaaaca gatttacatg agataatgga agctatacta gataataagt
    31621 taaaagatat agaaataaac tatagtgatg atgaagctgt gtgtgttatg cttacatcag
    31681 gagggtatcc agacagttat gaaaaaggga aaattataac tggtcttgaa aacttggatg
    31741 atgatatagt agtattccat agtggcacta agatgtttga tggtaatttg gttaccaatg
    31801 gaggaagagt aattggtata actgcaaagt caactacagt taaggatgca gcagaaaaag
    31861 tttatgaaaa cataaagaag ataaactttg aaggaatgca ctatagaaca gacataggaa
    31921 gataattttt ctaaaatgga ggataaggta tgttgaatac tgaaaataaa gactcaatgg
    31981 ttagaagagt attagttgaa aaaagagaag gttttgacct tgaggctaaa gctttaaaaa
    32041 aagacttagt agaaagttta cacatagata atatagaaaa tttaagaata ttaaatagat
    32101 atgatgtaga aggaatatca gaagaagttt atgaaaatgc agcaaagaca atattctctg
    32161 aaccaaattt agatgtagta tattatgaag aaataccaaa attaaatgat gaaagagtat
    32221 ttgcaataga atttcttcct ggtcaatacg accaaagagg tgattgggca gctcaatgtg
    32281 tacaaattgt aaatcaaggg ataagacctg ctattaatac agcaaaagtt tatatattat
    32341 caggcaaaat aactgatgaa gagttttcta aaataaaaga ttactgtata aatccagtag
    32401 atagtagaga agcatcttta gaaaaaccag aaacgttaaa aatggaaact gaaataccaa
    32461 caacagttga agtattagat ggatttatag atttagatga aaatggatta aaaacatttg
    32521 taagtgaaaa aggattagca atgactttag gagacttaca acacgttcaa aaatacttta
    32581 aagatacaga aaagagaaat ccaactataa cagaaataaa agtattagat acttattggt
    32641 ctgatcattg tagacatact acttttatga ctggaataga aaatgtaaag atagaagatg
    32701 gtaagtttaa tgatatagta aaagaagcgt atcaaatgta tcttaactca agagacaatg
    32761 tatatgtgaa cagacataaa gatatatgtt taatggatat agccactgtt gcagtaaaag
    32821 aattaaagaa aaatgggaaa ttaaatgacc tagatgaaag tgaagaaata aatgcatgta
    32881 gtataaacgt agatgttgaa gttgatggta agatggaaaa atatctagta atgtttaaaa
    32941 atgaaactca caaccatcca actgaaatag aaccatttgg tggagcagct acttgtttag
    33001 gaggagctat aagagaccca ttatcaggaa gaagttatgt ttatcaagca atgcgtgtta
    33061 caggaagtgc tgacccaaga acgactttag aagatacttt acctggaaaa cttatgcaaa
    33121 gaaaaataac tacagaagct gctcatggat atagttcata tggtaaccaa ataggtctta
    33181 caactgggca agttgcagaa gtatatgatg aaaactttgt tgcaaagaga atggaaatag
    33241 gtgcagttat agcagcagca ccaaaagaga atgtagtaag agaaagacct gaagcaggag
    33301 atgtaatcgt tcttcttgga ggaaaaacag gaagagatgg ctgtggagga gcaactggtt
    33361 catctaaaga acatagtgaa gagtcaatct taacttgtag tgcagaagtt caaaaaggag
    33421 atgctcctaa cgaaagaaaa atacaaagat tctttagaaa taaagaagtt gctcaaatga
    33481 taaaaagatg taatgatttt ggagcaggtg gagtttgtgt tgctatagga gagattgctg
    33541 atagcttaga tataaactta gacttagttc ctaaaaaata tgatggactt gatggaactg
    33601 agcttgcaat ttcagagtca caagagcgta tggcagtagc cataaaaaaa gaaaataaag
    33661 ataaatttat acaattagca gtagaagaaa acttagaagc tacacatgta gctacagtaa
    33721 cagatacagg atatttaaga atgttctgga atggaaaagc aattgtagat ataaatagag
    33781 agtttttaga tacaaacgga gtaaaacaaa ctactgatgt tcatgttact aaagtagatg
    33841 aagaaaatac attctttagc tctaatgaaa tagtaaaaga tgttaagtgt gcaagtatga
    33901 aagacaagtt tacaaaagta ctatctgacc ttaatgtttg ttctcaaaaa ggattagtag
    33961 agatgtttga taatacaatt ggtggtaata cagtccttat gccatttgga ggaaagtatc
    34021 aagcgactcc aacacaaggt atggttgcta agataccagt acttggagga gaaactaata
    34081 cttctacaat aatgacttat ggatacaatc caaaggtagg aaaatggagc ccattccatg
    34141 gtgccttata tgcagtagta gaatctgtgt gtaaattggt tgctattgga ggaaattata
    34201 gtacaacaag acttacattc caagagtatt ttgaaaaact tggtaacaat ccagaaaaat
    34261 ggggtaaacc attctcagca cttttaggag cattttatgc acaaagtaaa tttgagatac
    34321 ctgctatagg tggaaaagac agtatgtctg gtacatttaa agatattgaa gttccaccaa
    34381 cattagtttc atttgctgtt gacacagtag atgcgaagaa agtagtatct ccagaattta
    34441 aaaaagcaga ctcaaaagta gtaatgttat gtgtaaacaa ggcagaaaat gatgttgtag
    34501 attttgagga attaaagaga aacttagata aagtaagaga acttatacat ggaaataaag
    34561 ttttatcaac atatgcatta ggatttgcag gtgtaggtga agcaataagt aaaatggctt
    34621 ttggtaataa aataggattt aagtttagtg aagaggctga aaaagcattt acagatgata
    34681 aattatttga ggctagttat ggaaatatag tacttgaatt agcaaatgat gatttaagta
    34741 tgttagaagg atacaattat gttgtgcttg gaagtacagt taaagaagct agtatattta
    34801 tcaaaggtga agaacttgca ttagatgaat tatataaagc acattgtagt actttagagc
    34861 ctatattccc aactaaaaca gaagaagtaa aatcaaagat agaaactata agttatatat
    34921 cacaaggaga agctaaaaaa tcatcattaa gtatagctac tccaagagta tttataccag
    34981 cattccctgg aacaaactgt gaatatgact ctgcaagagc atttgaaaga gcaggagcta
    35041 atgcaagtat aagagtattt aagaatttaa cttataaaga catagaagat tctatagata
    35101 caatagtaaa cgaaataaaa tcttctcaaa taataatgtt accaggtgga tttagtgctg
    35161 gtgatgagcc agatggttct ggtaaattta tagctactgt atttagaaat cctagagtgc
    35221 aagaagctat aaatgaattt ttaactcaaa aagatggact aatgttaggt atctgtaatg
    35281 gattccaagt attaattaaa ttagggttag ttccatatgg agaaataaga gttccaagtg
    35341 aaagtgctcc aacacttact tacaataata ttggtcgcca tcaagctaag attgctagaa
    35401 ctagaatttc atctaataaa tctccttggt tagctcaaac taatgttgga gatattcata
    35461 atatagcaat ttctcatgga gaaggtaagt ttgttgccag tgaagatgtt atgagagaat
    35521 taattgctaa tggacaagta gcaactcaat atgttgactt taataatgaa gctacttatg
    35581 atattgagtt taatccaaat ggttcattct atgctgttga aggtataact agtgctgatg
    35641 gtagagtgtt tggtaaaatg ggacattctg aaagaattgg tgaggaagta tataagaata
    35701 ttataggtga aaaagaccaa aagatatttg aatctggagt taaatatttt agataaagct
    35761 ttgatgttta attgatagct agaatatata atttagatat ttagatttaa agagatgtaa
    35821 taatatgatg ataatcattt aatattacat ctctttttta tttatctaag aaataataag
    35881 ttcaacctaa gttaaaatac aagctgtgtt ataattaatt attaagtaaa attgaatatt
    35941 tattggaggg caacataatt aatgaaaata ttaataacag ggtctaatgg tcaattagga
    36001 aaagaattgg ttaaccaatt agaagcaatt aatcaaagta taaaccaacc aaaatatgtt
    36061 atattagcta caacaagaag tgatttggat atatccaatc agactaatgt agataatttt
    36121 attttgcata ataaacctga tgtagttgta aactgtgcag catatacaaa ggttgatgca
    36181 tgtgaagata atatcgaaat tgcatacaaa ataaatgctt taggagtgag aaatctagca
    36241 atagcatcag aaaaagttaa tgctaagctt attcacatat caactgacta tgtattcaat
    36301 ggtttttcta aatatccata tagggaagac aataaaactg aacccaattc tgtatatgga
    36361 aaaagtaaac ttatgggaga aaaatttgtt gaacaattct cacataagta ctttatattg
    36421 agaacagcat ggttatatgg taatgggaat aattttgtaa agaccatgat aaaactttct
    36481 ttagaaaata aagaggttaa tgtggttaat gaccagtttg gaactccaac aagtacagta
    36541 gacttagcaa aaattataat aagaatcatg gaaacagaac attatggagt ttatcatgct
    36601 acatgtgaag gtgagtgtag ttggtatgat tttgccaaaa agatatttaa actaaaaaat
    36661 atagatataa aagtaaatcc aattaagagt aacgaattta aatgtaaggc tccaagacca
    36721 ctatattctg tattagataa ttttatgttg aagttaattg gtttaaattc ttttagaaag
    36781 tgggaagaat ccattgaaga atatttagaa agatatgata taaatgaaaa gtaaatgtat
    36841 tattttgaga aaagaaaata agatattata aaaaatataa atatttcttt tatttatccg
    36901 ataatcataa tatgacaaaa acaaatttaa ggatggaaaa agatgaaagg aattatttta
    36961 gcaggagggt ctggtacaag actatatcct ataacaaaat gtatatcaaa acaaacactt
    37021 ccaatatacg ataaaccaat gatatattac cctatgtcgg ttcttatgtt ggcagggata
    37081 caagatatat taataatatc tacaccaaga gatataaaag catttgaaga attatttgga
    37141 gatggaagtt cactagggat taacataaca tatgcaattc aggagaaagc aaatggaatt
    37201 gcagaagcgt ttataatagg agaagaattt ataggaaatg acagagtgtc tcttgtatta
    37261 ggggataata tattttatgg atatggattt agtgaaaggt tggagaatgc agttaaaaga
    37321 aaaggagcaa ctatatttgg gtatcatgta agtaatccaa attcgtttgg agttgtagaa
    37381 tttgataagg attttaatgt attgtcaata gaagaaaaac ctacagttcc taaatctaat
    37441 tatgctatac caggattata tttttatgat aatgatgttg tagagatagc aaagctagtt
    37501 gaaccgtcag atagaggtga attagaaata acctctataa acagtgaata tctaaaacga
    37561 ggaaaactaa aagtggaact tctaggaaga ggtatggctt ggcttgatac tggtacacat
    37621 aaaggtctat tagatgcttc taattatgtt gaagtggttc aaacaagaca gggattgtat
    37681 atagcatgtt tagaagagat tgcatataca aaaggatata taagtagaga acaattaatg
    37741 aaactagcca agccactgat aaaaactgaa tatggtcaat atctattaaa attggctagt
    37801 gaagatatgt aatatggtag ataaaattta ttataaattt tattaaggga gtttattata
    37861 atgggaaaaa tcaatgttat aaagactaaa atagaaggat tatatatagt cgaaccaaat
    37921 gtattttatg atgaaagagg atattttatg gaaagctata ataaaaagga ttttatagag
    37981 cagggtcttg atatgtgttt tattcaagat aatgagtcta gctcaagaaa aggcgtttta
    38041 agaggaatgc attttcaggt taaatatcct caaggaaaat tagtcagagt tacagaggga
    38101 gaagttttcg atgttgcagt tgatttaagg aaagattcac aaacatttgg gaaatgggaa
    38161 ggtgtaatat tgtctgataa gaataaaaaa caattttata ttccagaagg gtttgcacat
    38221 ggatttttag ttttatcaga aaaagcagta tttagttaca aatgtacaaa ttattattac
    38281 ccagaatttg agaatggttt tatatggaat gataagcaaa tatctattga gtggccttta
    38341 aaaggaattg aagaaatatt tttatcagaa aaggacaaaa aacaaaaaag ttttaaagat
    38401 atttgtacaa gacgttaata aaaatttatt agaattaaca tttaggagaa aaaatatgaa
    38461 aaaaatactg ataactggag gagcaggatt tattgggagt aattttatac attatatgct
    38521 taacaagtat gtagattatt ttattgttaa cctagacttg ttgacttatg ctggtaattt
    38581 agagacatta atagaaatac aagacaattc caattataaa tttgttaaag gagatatttc
    38641 aaatagggag caggtgtata agctatttga agaagaaagg tttgatatag ttgttaactt
    38701 tgccgctgaa tctcatgtag ataggtcagt tgagaatcct gatttattta ttaaaacaaa
    38761 cattatagga acacaagtac ttttagatgc atctataaaa tatggagtta aaaggtatca
    38821 tcaaatatct acagacgaag tgtatggtga tttaccaatt gatagaaagg atttattctt
    38881 tacagaacaa tcttctatca atccaagttc tccatattca gcttctaaag catcagcaga
    38941 tttattggta aattcttatt atagaacata tggattgtta acaactatat ctaggtgttc
    39001 caacaattat gggccatatc attttcctga aaaattgatt cctcttatga ttttaaatgc
    39061 tttggaaaac aaacaattgc cagtatatgg gaatggggaa aatgtaagag attggttaca
    39121 tgtatatgac cattgtactg ctatagactt aataatacat aaaggaaata taggggagat
    39181 ttacaatata ggaggtcata atgaaagaag taatctagaa gtagtaaaag tgatattaaa
    39241 tcttttaggt aaatctgaag agttaatatc ctatgtaaat gatagacctg gtcatgattt
    39301 aagatatgct attgatgcaa ctaaaataga gaatgagtta ggttggaagg caaaatatga
    39361 ctttgatttg ggtataaaag aaactgttaa gtggtatata gaaaatgaat cttggtggaa
    39421 agctgtttta tcagtataac tttaaaattt agaaaggggc gttttatatt gaatgagata
    39481 agttcactaa ttaatatatt agcattgact caattatctg gtagtaatac tacaaatcaa
    39541 tgtaattgta catataataa ttcatctgga tttgatatga ttatgatgac cttgttaaaa
    39601 gcattatcac aaaataatca acatacttct aatagctaca atttattgtc taattctgag
    39661 aatttattta atgaacttga taatgtagta aataatacca ctagtagatt tatagatgtt
    39721 gatacaaaag ataaaaatgt aaagttgaga attgaaaatg ctgtagatca agcatcaaaa
    39781 aaatataacg tagattcaaa tcttataaaa gcaataatta aagttgaatc agattttaat
    39841 ccaaataccg tttcttctgc tggtgctaag ggtcttatgc aacttatgcc agaaaactgc
    39901 agagacttag gagttacaga cccttttaat atagaacaga atatagatgc tggaactaga
    39961 catattaagg aatacattga tatgtttgga gggagcatag aaatgggatt gatggcatat
    40021 aatggtggtc ctggaagaat gaaaagtaga ggagtagaat caatctctga cctgtataaa
    40081 atgccaaaag aaacacaaaa ttatattcct aaggttatga aatattatag agggtaggct
    40141 ttattatggg aataaatata gataagctaa acataagtcc aagtataaat atggaggtta
    40201 aaaaactaaa agagagtaat aaattttctg acaaattaaa ctctttaaaa caatcttctt
    40261 cattggaaga tataaaacga aatttggaaa gtgtaaaaaa ggcaggagaa aggcttgtac
    40321 ttactcaaaa ctatggcgat attagtatat ataaaaatgc agtaaaagaa tacctaaaat
    40381 ctgttgtaga taatatgtat tcacttgata aaaatagtag cttttgggag catcagtatt
    40441 ataagaatgt aaagattgtt gatagtaaac ttgaggatat gactagtaag ttaattagtg
    40501 aagaaaaaga aaacatagat attgtttcta ctgttgacat gatacaaggc ttattgatag
    40561 atatgtatag ataaggttat acaaggagaa aaattttatg aatagcaaaa aagaggatat
    40621 atatgaattg aattttgggc aattaataga agaaaaatct ggtgttatag aagaaaatca
    40681 aaatgttttt aatgcaaaag taaatgtagc tgtttctata ggaaatacaa cagaaacaat
    40741 aaaaaatata ttagatttaa aagaaggctc tataataaaa ttaagtaaga atgtggatga
    40801 aaaattagat atatatgcaa acgatagatt gtttgcttat ggagaaagca ttattacaaa
    40861 tgagaggtta tctgtaagat tatctaaaat agaagatcca gatgtaaatt agcattttaa
    40921 atagtatccc tttaggctat tctgtatgag tttagctaag gggatatttt tataaaaaat
    40981 ttaaaatatg tgtgaattat ataaatattt ttattttatg agcgataact aaattataag
    41041 tatgtaattt tggaggtgaa actatggata ttaaaaatgt aagttcaaac attgtagtga
    41101 ataattattt aaagaataca tctggtataa aaaaagtgga agaggttaaa aaaattaata
    41161 aaacagacaa gatagagtta tctaaatttg caaaggaaat ggcaagtgtg tcaaatatga
    41221 gtgatgagaa tattagaaat aaaaaagtcg atgaaataaa aaatgcatta gaaaatggaa
    41281 cttacaaaat aaaaccaaaa gaaattgcga aaaaaatgat agaggatatg cgaggataaa
    41341 gtcatgatta cagacttaaa agttataata tatgaagaaa aaaatataat tagtaagatg
    41401 atggaattat taaaagaaca atttgattat atagttgaaa aagacttaga aaatttaaat
    41461 atgttaaatc ctaaactaga agatattagc agggagcttg catccataga aataaagaga
    41521 agacaacttt ttggtgatga tatttcaatg agtgaagttg ttgaaaatag taatgatgga
    41581 tacttaaaag aaatatatac agacttgaaa cacattcttg ctttagctaa aaatcaacaa
    41641 gaatcaaatg attctctaat aaagaaagaa cttattttta cgaaaaaaat gattaatttc
    41701 ataaaaccag ctgataagaa aactacaact tataattctt atggaaatat tagaaaataa
    41761 atattaggag gagaaatatg gcaggattat tttccatttt aaatacagct aattcaggaa
    41821 tgaatgcgca ccaaaaagca atacagacaa tttctcataa tataagtaat ctagatacag
    41881 acggttattc taggcaaagg accgagtttg ccacaaatag tccaatgtat atgcctagtt
    41941 tgagtaattc aattgcaaga ggacaattag ggactggagt acatgtaacg gatgtaacaa
    42001 gagctagaaa tagtttttat gattaccaat ttagggcaga agctcataag tatggtaaga
    42061 cagtttcaaa atatgattat tacaatacta tagaaactat actaaatgaa ccatcagact
    42121 atggaatatc tgcaggtata gatgattttt ttaaaggatg ggagtcatta tcaaaggata
    42181 taaatagtga cagtaaaaaa agtctagtgg tagaaaacgc atctaattta gcaactttaa
    42241 taagtgaaag ttataagaag cttgataagt taaaagaaga tatagattct aatatagaca
    42301 ctgagataaa acaaataaat gacatgttaa aaagtcttga agatttaaat aaatctattg
    42361 atattatatc tggttctggc tctacaccaa atgatttact tgatgaaaga gatagaattt
    42421 tagataactt gagttttaaa ctagactttg aaaatagtga tgttaaaaat atgcttgcag
    42481 atggtaaatt agaacttaat gaattaaaaa atccagatgg aacttggaaa acaggtatat
    42541 cagggacctt acaaggcttg tttgaaatgc atggtaagat tgatacatat aaaagcgatt
    42601 taaaagatgt atctgatgga ttagctaaac agataaatga tgtttataat acgtctgcag
    42661 gaataacagt aacagatttt tttataactt caaatgtaga tggtgaagat attataaaga
    42721 taaatccagc tataaagagt aacccaaatg aattaaagct tactgctgat gaagcttcaa
    42781 aaattgctaa gttaaaggat gaaaaaatag atattggggt ggctggtgga aaagtaagta
    42841 caataagtga ccactataaa gcctttgcag aatcaatcgg tttagattct caaaaagtaa
    42901 atcaagatga agcaaatcaa cgtaaaatta taaataatat tgataactca agaatgagtg
    42961 tttcaggtgt atctttggat gaagaaatga cagagcttat aaaaatacaa cgttcatatc
    43021 aagctagtgc aaaagttatg tcaacagctg tgcaactact ggatgttgta ataaatggaa
    43081 taatttaata gaagggggaa tttataatga gagtcagtac aggtatgatg tctagcagct
    43141 atctaaactc actacaagat aatctacaaa gattggataa ggttaataga caagttaata
    43201 caacaaaaga aataaacaag ctatcagata atccatataa agcaataaag atattaaact
    43261 caaaaagtga aataaagact atggagactt atgttgagaa ttgtaaagat acagggaatt
    43321 ggttagaaac tactgatact gcattagatc agcttggaaa attattatca gacataaaga
    43381 aaactttggt ggcaagtggt gatggtgcat actcagatga tgaattaaag actcttagta
    43441 atgaaactaa tgaaaaaatg aaagaaattg caaatgcttt aaatgcaaca catgaaggga
    43501 agtacatatt ttcaggttca aatacggggg taccacctgt tgaatgtata gataatgctg
    43561 atggttcagt gagtttaaag tttaattcta gtctaaatct taataaatta aatgactctt
    43621 tgagtatgga tatagctcaa ggaataagtg tagattataa cgttaaattg agtagccttg
    43681 gatttgaccc aacaaaaact gacccatttg aagagttaaa taacatatca aaaaaattag
    43741 caaatccaga tgctgatgct ataaaagagc ttacaacaac ttgtttgggc gatatggaac
    43801 aactaataga aaatacagta aatgttaggt ctatatatgg gacaaaagca aatacagtag
    43861 aggctatgaa ggaaaaaaac gatgatagtt tgatacagtt aaaggatatt ttgtcacaaa
    43921 atgaagaaat agattatggt gaaaaacttg ttcaattaaa ggcagcagag ttaacttatc
    43981 aagcttcact acaaacagga ggaaagttat ttaatgtatc tatattagat tacatataat
    44041 aaaataacgc agatatgatg aaggttacat taaaaaaagg tatattaggt tttgaaaatt
    44101 taaaagaata tgagctttta gatatagaag ataatgatta tttaaaagaa tttaattcta
    44161 ctgaagaaga aggtatagga tttgttgttg tatctccatt tgagattatc aatgaatatg
    44221 aaatagtttt aaatcaggaa acaatagaaa aactagaagt gaaatctcca aaggatgttg
    44281 tattatttaa tataattact cttggtcaga catttgaaga atcaactgtg aacatgaaag
    44341 ctcctatagt gataaatata aaaactaatt ttggaatgca aataatttta caggatgata
    44401 aatattcaat aaggcatcca ttattgagag gtgataatgg atgctagtaa tttcaagaaa
    44461 aaaagatgaa gcagtactta taggagaaag catagaagta aaaattgtag gagtagatgg
    44521 aaacaatgta aaactagcta tatcagcgcc taacaatata agtatattaa gaaaagaaat
    44581 atacgaaaaa gtaaagagtg agaatataaa agctacaaat aaaaatataa aaattttaaa
    44641 gtcattaaaa taataagctt ggaggaagta atatatgtat ggagaaaatc cttataattc
    44701 ttataaacaa aatgctgttt ttatggcatc aaaggaacaa ttgttactta tgcttgttga
    44761 tggagcagta aaatatacca aaatagccaa aacagctatc ttagataaaa atacacaaaa
    44821 agcgcataga gagcttataa gagttcagga tatatttaca gaactaatgg ttacattaga
    44881 ccaaaatgca ggtcaatggg caaaggatat gtacagagtt tatgattttg taagatatga
    44941 actttcaaga gctaatatta gaaaagacat tcaaataata gataatgtgt tgcctgtaat
    45001 agaacaaatt aaagatactt ggcatgaagc tgataaaaaa agtaaagaag aaagaagtag
    45061 gtacaaataa tggaattaaa tttagctgaa ttagataagt tatcaaaaga ggaattacta
    45121 ttgatgcttg tagatggaac agtaaaatat accaatatat ccaaagaagc tcttttaaat
    45181 aacgactatt tgaaagcaca taatgaactt gtaagagttc aaaatatatt tacagaacta
    45241 atgactacat tagaccaaga tgcaggtcaa tgggcaaagg atatgtataa agtttatgaa
    45301 tttataaaat ctgaattagc aatagctgat gaaaataaag atattaaaat aattgatgat
    45361 atattgccaa tagtaaaaca aattagagat acatggcatg aagtttacaa tcaactaaaa
    45421 atataattgg atggagggat aaaggtatgt caagtataag tcctataaga gttacaggtc
    45481 tttcaggaaa ttttgatatg gaaggcataa tcgaagctag tatgattaga gacaaggaaa
    45541 aagttgataa agcaaaacaa gaacaacaaa tcgttaaatg gaagcaagaa atatatagaa
    45601 atgttataca agaatcaaaa gatctttatg ataaatatct aagcgtaaat tctcctaata
    45661 gtatagtaag tgaaaaagca tactcttcta caagaataac cagttctgat gaaagtatta
    45721 tagtagcaaa aggctcagct ggtgcagaaa aaataaatta tcaatttgca gtttctcaaa
    45781 tggctgaacc agcaaaattt actattaaat taaattcaag tgaacctatt gttcaacagt
    45841 ttcctccaaa tgctagtgga gcgagttctt taactatagg agatgtaaat ataccaatat
    45901 ctgaacaaga tactacaagt actattgtaa gtaagataaa ctccctttgc gcagataatg
    45961 atataaaggc ttcttatagt gagatgacag gtgaattgat tatttcaaga aaacagactg
    46021 gttcgtcatc agacattaat ttaagagtaa ttggaaatga caatttagct cagcaaattg
    46081 ctaatgataa tggtatcaca tttataaatg atgctggtgg aaacaaagtg gcaaatgtat
    46141 atggaaagaa tctagaagct gatgtaactg atgaacatgg aagagtaact catataagta
    46201 aagaacaaaa ttcatttaat atagataata ttgactataa tgtaaattca aaaggaactg
    46261 caaagttgac ttctgtcact gatactgaag aagctgttaa aaatatgcaa gcatttgtgg
    46321 atgattataa taaactgatg gacaaggtct atggtttagt tactactaaa aaaccaaaag
    46381 attatccgcc tcttacagat gcccaaaaag aagatatgac aactgaagaa atagaaaaat
    46441 gggaaaagaa agctaaagaa ggtatactta gaaatgatga tgagttaaga ggttttgttg
    46501 aagatattca atctgcattt tttggagatg gaaaaaatat tattgcatta agaaaactag
    46561 gtatcaatga aagtgaaaat tacaataaaa aaggtcaaat atcatttaat gcagatactt
    46621 tttcaaaggc tcttatagat gatagtgata aggtatacaa aacactagca ggttattctt
    46681 cgaattatga tgataaggga atgtttgaaa agctaaaaga tattgtatat gaatattctg
    46741 gaagttcaac ttctaaactt cctaaaaaag caggtataga aaaaactgct tctgctagtg
    46801 aaaatgtata ttcaaaacaa attgcagagc aagaaagaaa tataagcagg ttagttgaaa
    46861 aaatgaatga taaagagaaa agactttatg ctaaatattc agccttagaa tctttgttga
    46921 atcagtattc ttcccaaatg aattatttct cacaagcaca gggtaattaa ttatggataa
    46981 attaaaagaa aaattaaatt tatataaaga tatatcttta caaattataa atttgattga
    47041 aaaggaagaa tatataaaga taagtattaa gttaggtgaa agacaagaga ttataaatag
    47101 tgtaagtgaa attgatagaa atgattttat acagctttat aatagaatgg aattaattga
    47161 aattgatagc aggataagag atattttaca aggacaatta ttagaagtaa aaaaagaatt
    47221 acatgagtat aagcttacta aacaagtgaa cacaatgtat tataacttaa atagagaaaa
    47281 agtaaatatt tttaataaaa aagtataaag ttatgtacca tcttgtccga ttatataaat
    47341 gaagaattaa taaaaaagat taaaagtaga aatgacaagg atgtcaacta tactaaggag
    47401 ggtaaaataa tgagagttaa tacaaatgta agtgctttga tagcaaataa ccaaatggga
    47461 agaaacgtaa atgcacaaag taagtctatg gagaagctat cttctggtgt tagaattaag
    47521 agagctgctg acgatgctgc tgggcttgct atatctgaga aaatgagagc tcaaataaaa
    47581 ggtttagacc aagcaggaag aaacgttcaa gatggtatat ctgttgtaca aacagcagaa
    47641 ggtgcattag aagaaactgg taacatacta caaagaatga gaactttatc agtacaatct
    47701 tcaaatgaaa ctaatactgc tgaagaaaga caaaagatag ctgatgagtt actacaatta
    47761 aaagatgaag ttgaaagaat atcaagctca attgagttta atggtaagaa attacttgat
    47821 ggaagttcta cagaaataag attacaagtt ggagcaaact ttggaacaaa tgttgcagga
    47881 acatctaaca acaacaatga aataaaagtt gctctagtaa atacttcaag tataatgagt
    47941 aaagcaggta taacaagttc tacaatagca agcttaaatg cagatggaac ttctggaaca
    48001 aatgcagcta aacaaatggt atctagctta gatgtagctc ttaaagaatt aaatacatca
    48061 agagcaaaat taggggcaca acaaaataga ctagaatcaa ctcaaaacaa cttaaataat
    48121 actatagaaa atgttacagc agctgaatca agaataagag atacagatgt tgcttcagaa
    48181 atggttaact tatctaaaat gaatatatta gttcaagcat cacagtcaat gcttgctcaa
    48241 gctaatcaac aaccacaagg agttttacaa ttattaggat aaaataattt tgtatattgg
    48301 atatagaaga aagggtaagg ctttgcttta ctcctttctt tttttacaaa tatacttttg
    48361 aaaggaaaat ttatatgtta cttagtatag ttatgattgt taaaaatgaa gaaaaaatac
    48421 ttgaaaaaac tttaaaatca ttaaccacat taagaaattc tatagaaagt gaacttataa
    48481 tagtcgatac aggttctaca gatgatacta taaaaatatc taagaagtac acggaaaaag
    48541 tgtattttca taattggaat aatgattttt ctagtatgag aaatatatca atatcctatg
    48601 ctaaaggtga atggttactt atattagatg cagatgaaat actaattgat tcttcaacta
    48661 ttgtaaaatt ttttaaagat ggtcttgata aaaaatttaa ttctgcttca atacaattaa
    48721 aaaatcttta ctcatatgat aaaaaattat atggatattg ttcggtgctt agacttttta
    48781 aaaacgttga ttttagatac catggtaaag tacatgaaca accaatatac aaaaacccta
    48841 tatttaataa tgttgctgat tttgaacatt atggatattt gtttgaagat gaagaaataa
    48901 gattaaataa ggtgaaaaga aatgaaaaac tattatttca agagttaaaa gagaatgata
    48961 attctccata tacaaactat caattaggga aaaattttat tatattagga aaatatcagg
    49021 aggctttata ctatcttgag aaatcttatg aattatattc taaactagaa ttggcacctg
    49081 gttacttgat aacaagcctt gtaaaaacat atttatattt aggaaaacat aagaagtgtg
    49141 aaagattgtg cttaaagtat attaagaaag acatgaataa tattgatata tattattata
    49201 ttgcacaagc acaagtagat ttaggaaagt atgaaaatag tatagattcg tataagagat
    49261 atatttactt gttagataat tatgaaataa gtacacaagc aaatagttta ttttcagaca
    49321 ctgatactgt agggcttaga gatgaggcta ttattacact gataaagatt tattataagc
    49381 ttgaaaaata tgagtttgta atttcagagt ataataacat tgaagatgtg gaaaagaaaa
    49441 agaatgtgta ctttagctta tttatgtcac tatataaatt gaatagattt gaagatataa
    49501 aaaattatta taaagaagtt tcaaattcta aaattgaaaa aaatagtttt tatagaaata
    49561 tagaagaagt tataaataat attaaagagg atgaaaaaga gtatgtatat aaaatccttt
    49621 gtgacattga aggtaattat ggcattttaa atcaaataag gataaataaa tgtgtatcta
    49681 tagataaatg taaagaaata ctcaatgaag aaaaggaaat aatttatgca tatattataa
    49741 aaatagcttt tgaagagaag attgatttac tagatatttt ttatgaaatg gaatatgtat
    49801 ggatagagaa atatctaaaa tatcttttag cattagaaag aagttttaat ttagtactat
    49861 ataagtacat aattagtcgt ccaaatacat cagatatgga aaagataaga gtatataaag
    49921 ctatatctaa agtagtttta gaaagtataa gcttaagtga tgaaaaatat aaggagattt
    49981 tttatcttta tataatgtat tcttataagt acataagata catatatagc aatttaagtg
    50041 attttgaatt aataaaattt gtttgtaatg atttagataa gttcactata gaatttaagc
    50101 atctacttga tattaaaaat aaagtaaata atgctaatga aaaattagag tatatacata
    50161 gtatgaggaa attactaaat gagtatcctt tttataataa aataataaaa atacttatag
    50221 gtgaattaga agaaaatata aaagaaagtg aagaatttaa ggatttaaaa gatagtttta
    50281 ttattagtat agaaaatatg attgaaactg gcaaaataaa tgatgcgaaa ttattggtag
    50341 atgactattc aaaaacattt gtggatgata taaagatttt aaatataaag ggaattttat
    50401 acatgtttga aaataagctt gataaagctg attttatgtt aaaaaaggca ttatcattag
    50461 atgtagaaaa tgaagatact atatataata tagaatactt aaaaagttta agataagtca
    50521 aaaataggag aattgaagaa tgaatatacc aatagtggtt aatgaaaaat ctaaagacat
    50581 aataaaagat tggcaatata catgtacaaa tgaagatata aaatttacaa gcataataat
    50641 attgacatat aatcaaatag aatatactaa attatgtatt gaaagtatta ggaaatttac
    50701 cccaaagaat aaatatgaaa taataataat tgataataac tcttctgatg gaacagttga
    50761 gtggttaaag ggacaatctg atataaaatc tgtgtataat aacaaaaact taggatttcc
    50821 aaaaggatgc aatcaaggta ttgagttagc tactggtgaa aacatattgt tgttaaataa
    50881 tgatgtaata gttactccaa attggctcta taatttgaat aaagctttat ggtctcaaga
    50941 taatataggt gctgtaggac ctattacgaa tgcatgttca tattatcagc aaattgatgt
    51001 tgattataag tcaattgagg acatgtttat atttgcacac aaaataaata aatctaatat
    51061 taacctatgg aaatatagat tgaagttagt tggattttgc atgttaataa aaaaaactgt
    51121 tctggataat gtaggggttt tagatgaatt attcacacct ggaaattttg aagatgatga
    51181 tttatctttt aggatgatag agtcaggata taagttatta ttatgccagg atacttttat
    51241 acaccatttt ggaagtgtgt cttttaaaga aaatcgtaat aaatatacag atataatgta
    51301 tattaataga agaaaatttg aaactaagtg gggatttaat ccagaatata gtggaatgat
    51361 aagaacagat ttgatttcta aaatagatga tagtttggag gcaaatatta atgttttaga
    51421 aataggatgc ggtatagggg ctactctttt agaaataaaa aatatatata aaaatgcatc
    51481 aatatatgga atagaaatta gtgagtcagc aggtaaactt gcaaagtata gtgcagatgt
    51541 acttatagca aatatagaaa aagtaaaatt ggattataaa aaagattttt ttgattatat
    51601 aatattggga gatgtacttg aacatttgat tgacccatgg aagatagtca atgaattgaa
    51661 acaatatcta aagaaggatg gttatctggt atctagtata ccaaatatta tgcatgtttc
    51721 tgttttaaga aatcttgtgt taggtaattt tacatatgaa gattcaggaa ttttggacaa
    51781 gactcattta aggtttttta cattaaatga gataacaaaa atgtttaatg atagtaatta
    51841 cataattaat agtattacag ctactagtat gccactttcg gaaattgata atagcttcat
    51901 tgatacaata tgtaaggaaa gctgtaaaga aatgagacaa cagtacgaat cttatcaata
    51961 tatagtaaaa gcctctaaaa tcctagatac taaaagatat tctagtatta gtatgattaa
    52021 tttaaaatac gctttaatga gaattgataa tgaattagat gtagatagta atttaagtta
    52081 tatatttgat aattataata taactgaacc atgttttgtt gatgatattg aatatctaat
    52141 tgatacattt gtgataaata aagcaactct attaaataga ttaggagttg aggcatttaa
    52201 cagaggtcta ggagatttta gtataaacat gtttatgaca gtattagaga tggatagaga
    52261 tgatattgac tcaatatata atataacaag tgtattatgt gaattagaag aatttgatac
    52321 tgcttataat ttaattaaaa atagtagcga aaaagtacag agtgaaagtg atatacaaga
    52381 gatactttgt tttattgagg ggaagaaata tgaataatga taagatatgt tttataacct
    52441 gtgtaaatga tgaaaggctt tatgaagaat gtcttgtata tataaatagc ctagacattc
    52501 ctaaaaactt tagtatagaa acagtagctt taagaggagc cgattctatt gcctctgctt
    52561 ataataaagc tatgagagaa acagattcta agtataaggt atacttacat caagatactt
    52621 ttataattaa taaaaatttc atacatgata ttttagagat atttaaaaaa aatacagatg
    52681 tcggaatgat tggtgtagct ggagtaaaag atatgcctgt gaatggaatt tggtgggatt
    52741 ctgataaaaa agcagggaaa gtatatgata gtcatacagg taaaatggat atttatgaat
    52801 ttagtaactt tgaagaatgt tttaatgaag ttatggctat agatggatta attatgatta
    52861 ctcaatatga tgtattttgg agagaggata agtttgatgg gtggcatttt tatgatttgt
    52921 cacagagtat ggagtttaga caaaataaat acaaagttat tgtaccaaaa caaaataagc
    52981 cttggtgtat gcatgattct ggagtagtaa atataaataa tggatatgat gaatatagga
    53041 taaaatttgt aaaagagtac ataaatcttg atttatatag agaaaatttt tatgaatttg
    53101 ataagaagaa agcaagtacc atacttaaga gtaataagca tgtaatggat tgtatgaaag
    53161 aggagcttac agaacaatta agattaaaga attacaagga tgttttaggt attgtttcta
    53221 aaattgctca tttttctgtt tttaatcata ctggtttgta ttcttgtgaa gaatgtgaga
    53281 gagcattgct agaatgtgca aataatttac cagaaataga aaatacgaaa ttatttacaa
    53341 aaaaagattt aaaaagaaga aaagtattac atgttttaag tgaaggatat tcaacaggag
    53401 gtcatacaag actagttaaa aattggattg aaattgacaa agactctgtt cattcagttg
    53461 taactacatg gcaatcagat acactgccag actggttatt aaaagcaata aatgatagtg
    53521 gtggcgaagt tatatctttg gatttaattt cagatgatta tttaaaaaga gctgtagctt
    53581 tgaggaaaat agcttatgac tgggctgata tagttatttt atatgttcat atgtgggata
    53641 caatacctgt tgttgctttt ggagttgatg ggaaaatacc tattttacat gttaatcact
    53701 atgatagtac tttttggtta gggacaagta ttgcagatat tgtcattgat tttagcgaaa
    53761 ctagtcaatt aattacatta gataaaagag gcatagataa atctgaaata ttaccaatgc
    53821 cattaaaaga aaaagaagta ttttcaaaat ctgaagttag aaaacgatat aatttagagg
    53881 atgatgtgac aataatattg actattgcta gtgaaagaaa atacaatagt gtaaatgata
    53941 ttaattactt taaaattgct aaaacattga ttgaaaatac agataataca attattattg
    54001 ttgtaggagt tgatacagag aatctctatt ggaaaacttt aagtaatgtt acagataata
    54061 gagttatatg ttttggaaca acagcaaata tagatgattt ttataagatt tcggatatat
    54121 acctagagtc tttcatggta ggaagtcata cttcaaaatt agatgcaatt ttatatgatt
    54181 taattcctgt taagtttaaa aatgaagttt caccaatttt tacaaatatg tggaatttta
    54241 tagataagtt tgattataag aatataaaag aggttataga catgataaga aggtacaata
    54301 ctagtgaatt tgacagggat aagataaaag caaaacaatc ggaaataaaa cattttgtta
    54361 tagataaaca ttgtattgaa ataaaaaact atatagacaa aatatataat gaagttaatc
    54421 atcataaagt taagtatgat ttatataaaa atgatgaaat cgaggactat cagctttttg
    54481 tagcattata tggctatatg gtaaaaaata aaaacatgtc ataggtggta aaatgaacaa
    54541 tattcatatc aataaatttg aaaaaagctg tgaaaaaaac tgggattatt ttgtagaaaa
    54601 taaatctata aatggtacat ttctgcaaac tagaaatttc cttaattatc atgaggaggg
    54661 aaaattcact gaccactcat taataatatg taaaggtgaa gaaatactag cagtcgtacc
    54721 tgcatgtgaa acttatgaag atggtgaaag aatatttttt tctcatttag ggagtacttt
    54781 tggtggcatt ataataagaa gtggttttaa tgatattagg catgtaaatg ccattacaaa
    54841 taagttagaa atatatttac aggaacaaaa tttcgataaa gtatatttaa aaaatacatc
    54901 aagaatattt tgtaaagagg atacttcatt actagaatat tttttattta agaataatta
    54961 catgttttat gatgaattaa gtatgttttt agaattaagt aagtataaag aagatatatt
    55021 agccaatttt aaatacagta aaagaagaga ttttaaatat tctttgaaga ataatcttac
    55081 ctttaaaaga ctgtatagtg atgaagatat agtttgtttt tttaatttac tttctaattg
    55141 tttagatagt aaaggagcta aagtaataca ttcagtagaa gaacttttgg agtttaaaaa
    55201 taatagatta aaagatattg tagaatttta tgcagtatat attgatgaca ttatgatttg
    55261 tgggagcatg gtatttaact ttcaagaaag agtttatcat actcaatatc tagcatcaaa
    55321 ttcgacatat aataaatatt atccaatgat atttttatac tataatctaa taaaagatgc
    55381 tttagagaga aattttaagt atctttcttt tggaattagc actctaaata gaggcaaata
    55441 cttagatgag ggtttagcta catttaaaga aggttttggt tcaagtgcaa tttgtaacag
    55501 aacatactat aaaaatataa ataagtgaga atcatgtttt atagagtttt tagagtatag
    55561 taaatatcta gtactataat attacataaa aataattagt ttataaagaa ggtttgtata
    55621 tgaaaagatt aatgatttta ggctcaatta agtattttga gaatattgtt atgtcagcta
    55681 aaagagaggg aatatacaca attgtttgtg ataatagaat tgatactcct gcgaaaaaga
    55741 tttgtgatga agcaatagat gttgatgtat ttgattttga taaaataaag aatatagcta
    55801 tagataaaaa gatagatgga attttaactg gatttacaga ttctttaatg aagccatatg
    55861 tttatattgc aaatgaacta aatttgccat gtgtgatttc ttgcaatcaa cttgaatctg
    55921 taactaataa ggctgttatg aaaaattatt ttaaaaataa tggaatacct acaactgatt
    55981 attgtattat tgaaagtttg aaagatttag aaaaaataca aggattaaat tttccacttg
    56041 taataaaacc tgttgatggt tatggttcaa gaggtgtata ttttgtatca aacatagaag
    56101 aattaataga aaaatttgaa tattctaagg aagcctcgca agatggacaa gtaataattg
    56161 aggaatttta tgaaagtgag gaaatacaag gactagcttg ggttcataat ggagagtcac
    56221 atgtatttta tattggagat agagagcttg taaatataca tacaggaaga gctggaaaac
    56281 cagatagatt aatttatcca tcaaagtatt gttttcaata tgaagaagaa ataaaatcca
    56341 tatatcaaaa tataacgcag gcttttggca taaaaaatgg acctctttat gtacaaatgt
    56401 tagtgggagc atatggaata aaggtatctg aagtaatgcc aagattacct ggaggttgtg
    56461 actatatggc tatttctcaa ataacagatt tagacatagg aagtttattt gcaaacttta
    56521 gtgtgaataa tgaagtaaat tttgatgaaa taaagaaata taatatgcat ctatcaaaat
    56581 gtgtatatgc attaccaata tacataagac ctggcacaat aaaaaagata aataatatag
    56641 ataaaataga aaacttagat tatgtcactc aatttctatt actagtaaaa gaaggagata
    56701 caataggatt atatgaagat gagagacagg attgtggaag agtttatgga gtagcaaata
    56761 atgtatttga tgcaaatata aagaagaaat gtattcatga aatgatagaa atacttgatg
    56821 aaaataatga aaatatgata gaaaattttt aaataaggga gtatgataga tatgaagcta
    56881 ataactcatg aacaaattat gtcattaaac atttctcaga aacaaggata cgagtggata
    56941 aaagaatcct ttttaacaaa aaaagattgt caattaccac caaaaataag catggtagaa
    57001 gaagggcatg tattttacaa tgtaatgccg tccctaaatc ataatcataa gattggtgga
    57061 ataaaaatgg ttacaagatt gccagggaga aatccaagtt taaattcaga gatatttcta
    57121 tataatatgg aagatgctaa attaaaggct attttagatg gcaattatat tacagctttt
    57181 aggactgctg ctgttgctgc cttggctatc gatacatttg ctaaaaaaga ttttaatact
    57241 attgctatta tgggtttagg tgtaactgct tctgctataa tgaatatgct ttcatatact
    57301 cttaaagata aaaatataaa tataaagtta cttaaatata aagaccaagc agagtcgttt
    57361 gtaaaaaagt atgaaaaaaa tgaacaattt agttttgaaa tatgtgacac acatgaagaa
    57421 cttattgtag attcagatgt agttatttct actgtgacat atacagatga atatttatca
    57481 gaatttaaat ggtttaaaaa aggtgcttta ttattacctg tacatgttat gggatttgga
    57541 aatgttgatt atctatttga taaagtatat ttggatgatt ataatcattt gaaacatcta
    57601 gaaaatatag atagatataa atattcttgt gaattttatg atgttttaag taaacaagct
    57661 aaaggaagag aaaatgatga tgagagaata atatcttaca atataggttt gtcaatacat
    57721 gatatattat ttgcaaataa aatttacgaa ttgtgtgaaa cactaagctg tgaagaaata
    57781 gaattaaaag aaccaaaaga aaaatattgg atatagttta tttcattcat atttacaata
    57841 gtttattgta aatatgaata tgaagtaata tattgttaga aaattagcat gaataggata
    57901 gataaattta tggataaata taatttaatt aggttcgtat ctattggaaa cgaatatata
    57961 ggaaatttag ttgcattaga atcaaataaa gcaatacctt ttgatataaa gagagtttat
    58021 tacatatatg atgtaccaaa caatgtaaag agaggatttc atgcacacaa aaacttggaa
    58081 caagtactag tttgcctaaa tggaagtgtg aagataaaat gttatgatgg tgtaaaagag
    58141 actatattta atttacataa aaatgatgtt ggattatata taggaacggg agtgtggcat
    58201 gagatgtttg attttgatga aaaaactgtt ttaatttcta tagcatctga acattataat
    58261 gaagaagatt atattagaga ctataaagaa tttttaaagt atttaaataa tatgtagtta
    58321 ttatacagtt agtgatgtat tttcttaatt aaataaattt aagatagcca catagagaat
    58381 agatagatta ggatgtgaat ttattgttgg ttaaatttaa taatttatta aaacagttcc
    58441 aaacccataa agaagaatat gaacagaaga ttaaggatgt aatggaaagt ggatattata
    58501 tccttggaaa tgaagtaaag cattttgaag aagaatttgc taactatgta ggtagcaaat
    58561 attgtgttgg tttagccagt ggtttagatg cactgataat tgcttttaga gcattaggaa
    58621 taagcgaagg tgatgaagta atcgtgcaat caaatacata tattgctagt gttatgggaa
    58681 tcagtataaa tggagcaaca ccagtatttg tagaaccaaa tgaatattat aatatagatt
    58741 catctaagat agaagaaaag ataaatagca aaacaaaagc tatattagta gtgcatttat
    58801 atggtcaagc aagtaatatg aaagatatac aagaattatg tgataagtat aacttgtatt
    58861 taattgaaga ttgtgctcaa tctcatggag ctaaatatga tggaaaaatg acaggaacat
    58921 ttggtgatat aggttgtttt agtttttatc caagtaaaaa tttgggtgca tttggagatg
    58981 caggagctat agtaactagt aatgaggaaa tagctgataa agtgaaaatt cttagaaact
    59041 atggctcaga aaagcgatat tataataaat ttgtcggata taattcaaga ttggacgaaa
    59101 ttcaggcagg tttattaaga gtaaaactta aatatctaaa tgaaattgaa attgaaaaat
    59161 caaaaatcgt agatagatat cttaatgaaa ttaataatcc caaagtcata cttccaaaga
    59221 tagaaaataa ttgtactcat gtatggcatt tatttgtttt acaagttgaa aaacgagata
    59281 catttatgga atacttaaag aaaaatggta tagagtctat aatacattat ccgataccac
    59341 cacatctatc tgaagcatat gcttatttgg gatataaaaa ggggagtttt aatatagctg
    59401 agaactattc agataaagtt gttagtatac ctttatttat cggtatggaa gatgaagaag
    59461 ttagttatat aattaaaatt ataaat
//
LOCUS       DAMJYC010000012        59264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJYC010000012 DAMJYC010000000
VERSION     DAMJYC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.012"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            774..2444
                     /locus_tag="PC472_000835"
     CDS             774..2444
                     /locus_tag="PC472_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2726185.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRINYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2566..3300)
                     /gene="pflA"
                     /locus_tag="PC472_000836"
     CDS             complement(2566..3300)
                     /gene="pflA"
                     /locus_tag="PC472_000836"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2726186.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3460..5691)
                     /gene="pflB"
                     /locus_tag="PC472_000837"
     CDS             complement(3460..5691)
                     /gene="pflB"
                     /locus_tag="PC472_000837"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2726187.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5984..7045
                     /locus_tag="PC472_000838"
     CDS             5984..7045
                     /locus_tag="PC472_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2726188.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLTVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7136..8749
                     /locus_tag="PC472_000839"
     CDS             7136..8749
                     /locus_tag="PC472_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2726189.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9080..11224
                     /locus_tag="PC472_000840"
     CDS             9080..11224
                     /locus_tag="PC472_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2726190.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            11312..11647
                     /gene="spoIIAA"
                     /locus_tag="PC472_000841"
     CDS             11312..11647
                     /gene="spoIIAA"
                     /locus_tag="PC472_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2726191.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11661..12089
                     /locus_tag="PC472_000842"
     CDS             11661..12089
                     /locus_tag="PC472_000842"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2726192.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            12112..12876
                     /gene="sigF"
                     /locus_tag="PC472_000843"
     CDS             12112..12876
                     /gene="sigF"
                     /locus_tag="PC472_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2726193.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            13156..13593
                     /gene="spoVAC"
                     /locus_tag="PC472_000844"
     CDS             13156..13593
                     /gene="spoVAC"
                     /locus_tag="PC472_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2726194.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            13760..14773
                     /gene="spoVAD"
                     /locus_tag="PC472_000845"
     CDS             13760..14773
                     /gene="spoVAD"
                     /locus_tag="PC472_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2726195.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            14787..15149
                     /gene="spoVAE"
                     /locus_tag="PC472_000846"
     CDS             14787..15149
                     /gene="spoVAE"
                     /locus_tag="PC472_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2726196.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            15332..16348
                     /locus_tag="PC472_000847"
     CDS             15332..16348
                     /locus_tag="PC472_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2726197.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            16723..17565
                     /locus_tag="PC472_000848"
     CDS             16723..17565
                     /locus_tag="PC472_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2726198.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            17580..18044
                     /locus_tag="PC472_000849"
     CDS             17580..18044
                     /locus_tag="PC472_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726199.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            18031..19203
                     /locus_tag="PC472_000850"
     CDS             18031..19203
                     /locus_tag="PC472_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2726200.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            19247..20182
                     /locus_tag="PC472_000851"
     CDS             19247..20182
                     /locus_tag="PC472_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2726201.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKFPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(20456..23137)
                     /locus_tag="PC472_000852"
     CDS             complement(20456..23137)
                     /locus_tag="PC472_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2726202.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGGSTTP
                     PDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            23351..24049
                     /gene="yunB"
                     /locus_tag="PC472_000853"
     CDS             23351..24049
                     /gene="yunB"
                     /locus_tag="PC472_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2726203.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(24261..25268)
                     /locus_tag="PC472_000854"
     CDS             complement(24261..25268)
                     /locus_tag="PC472_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2726204.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            25496..26380
                     /locus_tag="PC472_000855"
     CDS             25496..26380
                     /locus_tag="PC472_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2726205.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            26582..28132
                     /locus_tag="PC472_000856"
     CDS             26582..28132
                     /locus_tag="PC472_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2726206.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            28161..28808
                     /locus_tag="PC472_000857"
     CDS             28161..28808
                     /locus_tag="PC472_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2726207.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(28950..29210)
                     /locus_tag="PC472_000858"
     CDS             complement(28950..29210)
                     /locus_tag="PC472_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2726208.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            29427..30710
                     /gene="hflX"
                     /locus_tag="PC472_000859"
     CDS             29427..30710
                     /gene="hflX"
                     /locus_tag="PC472_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2726209.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            30718..31395
                     /locus_tag="PC472_000860"
     CDS             30718..31395
                     /locus_tag="PC472_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2726210.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            31481..31924
                     /locus_tag="PC472_000861"
     CDS             31481..31924
                     /locus_tag="PC472_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2726211.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            32229..32873
                     /locus_tag="PC472_000862"
     CDS             32229..32873
                     /locus_tag="PC472_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2726212.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(32959..33336)
                     /locus_tag="PC472_000863"
     CDS             complement(32959..33336)
                     /locus_tag="PC472_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2726213.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(33336..33722)
                     /locus_tag="PC472_000864"
     CDS             complement(33336..33722)
                     /locus_tag="PC472_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2726214.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            33901..34656
                     /gene="nadE"
                     /locus_tag="PC472_000865"
     CDS             33901..34656
                     /gene="nadE"
                     /locus_tag="PC472_000865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2726215.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            34739..35458
                     /locus_tag="PC472_000866"
     CDS             34739..35458
                     /locus_tag="PC472_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2726216.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(35625..36629)
                     /locus_tag="PC472_000867"
     CDS             complement(35625..36629)
                     /locus_tag="PC472_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2726217.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            36895..37791
                     /locus_tag="PC472_000868"
     CDS             36895..37791
                     /locus_tag="PC472_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2726218.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            37929..39191
                     /locus_tag="PC472_000869"
     CDS             37929..39191
                     /locus_tag="PC472_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726219.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            39267..40460
                     /locus_tag="PC472_000870"
     CDS             39267..40460
                     /locus_tag="PC472_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2726220.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            41089..41883
                     /locus_tag="PC472_000871"
     CDS             41089..41883
                     /locus_tag="PC472_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2726221.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            41978..43318
                     /locus_tag="PC472_000872"
     CDS             41978..43318
                     /locus_tag="PC472_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2726222.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNAKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            43455..44687
                     /locus_tag="PC472_000873"
     CDS             43455..44687
                     /locus_tag="PC472_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2726223.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            44727..45869
                     /locus_tag="PC472_000874"
     CDS             44727..45869
                     /locus_tag="PC472_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2726224.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            45880..46674
                     /locus_tag="PC472_000875"
     CDS             45880..46674
                     /locus_tag="PC472_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2726225.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            46685..47707
                     /locus_tag="PC472_000876"
     CDS             46685..47707
                     /locus_tag="PC472_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2726226.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            47806..49182
                     /locus_tag="PC472_000877"
     CDS             47806..49182
                     /locus_tag="PC472_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2726227.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(49514..49858)
                     /locus_tag="PC472_000878"
     CDS             complement(49514..49858)
                     /locus_tag="PC472_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726228.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            50346..51356
                     /locus_tag="PC472_000879"
     CDS             50346..51356
                     /locus_tag="PC472_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2726229.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAIEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            51492..51980
                     /locus_tag="PC472_000880"
     CDS             51492..51980
                     /locus_tag="PC472_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2726230.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            52156..54084
                     /locus_tag="PC472_000881"
     CDS             52156..54084
                     /locus_tag="PC472_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2726231.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            54261..54671
                     /locus_tag="PC472_000882"
     CDS             54261..54671
                     /locus_tag="PC472_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2726232.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            54693..54995
                     /locus_tag="PC472_000883"
     CDS             54693..54995
                     /locus_tag="PC472_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2726233.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(55212..55904)
                     /locus_tag="PC472_000884"
     CDS             complement(55212..55904)
                     /locus_tag="PC472_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2726234.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(55930..57117)
                     /locus_tag="PC472_000885"
     CDS             complement(55930..57117)
                     /locus_tag="PC472_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2726235.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQRATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            57453..59252
                     /locus_tag="PC472_000886"
     CDS             57453..59252
                     /locus_tag="PC472_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2726236.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGSA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
BASE COUNT        22331 a         7032 c        10534 g        19367 t
ORIGIN      
        1 tttgtagtgt aatcgtattt tttattaatt cttacaaaat caatgaaaat aagtatatcc
       61 attttaaagt acataaattt aaaaatccag aaaaatatac ttaaattatg taagtaaaag
      121 aggggttgaa tcagttgaaa atgtaatatt agtaaatagt acatcaatcc tatataccct
      181 gtaagcaact ctactttcat gtactaaaaa tgtacctatt ttattaactg aaaagaataa
      241 attaaatgta agtacataag aagaaagaaa aaattaaaaa caaaaaatat ttatataaat
      301 aaatataaaa aatgatataa tccctattaa aaaatttata gatgatgaag aaatatcaaa
      361 atcttatatt atagtaagag gtacttcttt gccaaaatct atagaaaagc aataccaaat
      421 ccaaagagaa taggcggaga aaaaaataaa ataaattcaa gagtaataga agagttttat
      481 aggataaaga actgagaact ttttatataa tgaaaaatag aattttaaga gaaaatgatt
      541 taataaatga tttaacatgg tgttttagca ggtaaaaata aatcaccagt aatgattata
      601 gaaaaattac tttatgaatc tcaaaaaact tagttaagag taagagattt gataagataa
      661 ctaaagttgg ggaaatgtgt ttgatgagat aaaaaattag taaaatagta taatctaaat
      721 aaggatattt tgttgattaa actacactaa tagtaatatt gtagggggaa aagatgagag
      781 aagaatcgaa ctctttaagt ttagaccata tagaaatttt ctttgaactt ttatctcaaa
      841 gtacagaaga ttatatattt ttttgggata taaataggaa taaatttaaa atttcttcag
      901 caatttttga tgaatttaac ttaagtaaag agatagaatc tgatttggtc aattgttgga
      961 gtaaaattgt ttatccggat gatgttcaaa tatggaaaga tgatatacaa gaattactac
     1021 atggaaaaaa aggtgagcac aatttagaat ataggttgat taataaatat gaagaaatag
     1081 tatggatttc ttgtagaggt aaagtttatg tgtcggatga cccaaaaact atatttctag
     1141 ttgggagaat aaagaatatt ggtgaaaaaa ataaatttga ttctatcaca ggaacgtgga
     1201 atagagaaca atttgaacat agaataaatt atcttataaa agagaaaatt tataaaaatg
     1261 gtgctatgtt tatcatggat attgataatt ttaaaaatat aaatgagaaa tatggacatt
     1321 cttatggtga caaagtattg cgtgcaattg ctactgaagt attagaatat cttccaaaag
     1381 atgtaagatt gtataggtta gatggagatg aatttgcatt tttttaccct atgtgcacta
     1441 aagaaactat agagaaaata tatgaaaaga tacagatgta tacaaatact cagcatgaaa
     1501 ttgaatcaaa taagtactat tgcacagtga ctgcaggagt tgctatgtat ccagaagatg
     1561 gagataatta tttagattta tttaaacatg ctgatattgc attggatata gcaaaaatta
     1621 gtggtaaaaa tagaattaag tttttttcac aagagctcta tgaaaataaa ctaaaggtaa
     1681 tttcaatgca acaaaaacta agagagtgcg ttgaaaacaa ttttaatgat tttgagttgt
     1741 tttttcaacc ccaggtaaat gctgttacaa aagaggtaat aggggcagaa gtacttttaa
     1801 gatggcattc gagtacttat ggagaagtat ctcctgttga atttatacca atactagaac
     1861 agagtaattt aattatacca gttgggaagt ggattataaa agaagctgta aaacagtgta
     1921 aagaatggca taaaataaat ccagacttta aaatatctgt aaatgtatcg tatattcagt
     1981 taaaggaaga tttttttaga gattttatag tagaatgttt agtagagtat caattaagac
     2041 ctgaattttt aattttggaa ttaacagaga attgttggat accagatata aatttactaa
     2101 atgataaatt tattagttta aagggtatag gagtatatat tgcaatagat gactttggta
     2161 caggatattc atctttaaat taccttaaag aactgtctgt taatataata aaaattgaaa
     2221 gaagttttgt taagaatatt acatataata gttatgaata tacttttctt gaatatatta
     2281 ttaaattagc tcatattata aatttaaaag tttgtgttga gggaatagag tcttatgaag
     2341 agtatgatat agtaaaaagc ttaggtgtag atattattca gggtttttta tttggaagac
     2401 ctgtaagtgc ctctgaattt tataaatttc agctatcaaa gtgatgtata taaaatgaga
     2461 tatatagtat ataaaaatag ggcaaagtgg aagttttgaa gtttattaaa aatttacact
     2521 ttaccctatt ttgttatgtc ttctataaag gattaatata ctacactaac tattatatac
     2581 tttcactcca aatttagaga ttatttcact agctttttgg acatcttcct tagtggcatc
     2641 aggaacatct tttagttcat aatcgatacc taaactttcc catttgtgta ttcccataga
     2701 atggtatggt agcaattcaa atctttctac atttttcaat gtagctacaa attgacctag
     2761 tttttctaaa ttatctatat catcagtaat gccaggaact aaaacatgtc ttatccatac
     2821 aggaatattt cttgattcta agtgtctagc aagttttaat gccttatcca taccaacacc
     2881 agtcaaatca atagattttt cctcaaccat atgttttaaa tcaagtaaaa ccaaatcaat
     2941 attatctaaa actgggtcta tagtttctat atctacaaat ccagatgtat ccaagcaagt
     3001 gtgtatatca ttttcttttg cttttttaaa taattcactt aaaaattctg gttgaagagt
     3061 ggattcacca ccagaggcag ttatacctcc accagaaaac ttcataaaag aagtatactt
     3121 aagagcatcc gttattattt catcaactgt gtattctttt cctgatttaa tatcccatgt
     3181 atccctgtta tgacagtatt tgcatctaag aggacacccc tgaaaaaata atatatatct
     3241 tattcctggt ccatctacag taccaaacgt ttctatagaa tgtatctttc ccttaatcat
     3301 agagttcacc tcacaatata ttttaataac cttatcaata gttataatat ttctctaaaa
     3361 aaggccatct tataccagaa ataattcaaa tataagatag ccttttattt ttatttttta
     3421 cacagtagta atcttagtta aagtataaaa atttaaagac taagccattt taccgtggaa
     3481 tgttctgttt ataacatcta attgttgctc tttagttaat tttatgaagt taactgcata
     3541 tccagatact cttatagtta attgtggata ttcttctgga tgttccatag catcttctaa
     3601 agtagctcta tcaaatacat ttacatttaa gtgatgagca ttttgagcga agtatccatc
     3661 catcattgca gaaagattgt ttattctttc agtcatatct tttcctaaag cagcaggtac
     3721 tatagagaaa gtattagata taccatcttg agcatgttca tatggtaatt tagcaactga
     3781 agataatgaa gctaaagcac cattagtatc tctaccatgc attgggttag caccaggagc
     3841 aaatggagca ccagctcttc taccatctgg tgtattacca gttttcttac cataaacaac
     3901 atttgaagtt attgtaagta tagattgagt tggataagaa tttctataag ttttattctt
     3961 tcttatttta ttcataaagc tttcaactaa ctctacagct atactatcaa ctctatcatc
     4021 attatttccg tattttggat aatctccttc tatttcaaaa tcaacagcta ttccttgttc
     4081 atttctaata gttttaacct tagcatgttt tatagcagat aatgagtctg cacaaactga
     4141 taatccagcc ataccacaag ccatagttct aaatatatct ctatcatgta atgccatttc
     4201 taatgcttca taagaatatt tatcatgcat ataatgtatt acatttaaag tatttacata
     4261 taatgttgct aaccaatcag tgaatggttc aaatttactc ataacttcat cataatctaa
     4321 gtactcagaa gttataggtt caaatcttgg tccaacttga acaccagatt tctcatctac
     4381 accaccattt atagcgtata ataaagtttt agctaagtta actctagctc caaagaactg
     4441 catttgttta ccaattctca ttgcagatac acaacaagct ataccatagt catctcccca
     4501 gtatgctctc attaaatcat cattttcata ctgtactgaa cttgtatcta tagatacttt
     4561 agaacagaaa tctttgaatc cttgaggtaa tttagttgac catagaactg ttaagtttgg
     4621 ttctggagaa ggtcctaatg tatatagagt atttaacatt ctaaatgagt ttttagtaac
     4681 taatgttcta ccatctattc ccatacctcc tatacactca gttacccaag ttgggtctcc
     4741 agagaatagg ttattgtaat caggagttct taggaactta accattctta atttcataac
     4801 aaaatgatcc atatactctt gtaattcttc ttctgttacg attccagctt ttaagtctct
     4861 ttcaaagtat atatctaaga aagtagatgt tctacctaaa gacattgcag caccattttg
     4921 gtctttaaca gcacctaaat aaccaaagta taaccattgt acagcttctt ttgagttagt
     4981 tgctggtttt gagatatcaa atccataaga ttcagccatt tttttaagtt catttaaagc
     5041 tgatatttgc tcagttattt cttctcttaa tctgataact tcctcatcca tgcaagaaac
     5101 ttctaatgat ttcttttgct catttttatc ttctattaaa gcatcaacac cgtatagagc
     5161 aactcttctg tagtcaccaa ttattctacc tctaccataa gcatctggaa gacctgttat
     5221 tataccagat tttctagcag ctctcatttc tggagtataa acatcaaaaa ctccttggtt
     5281 gtgtgttttt ctgtatttag taaatatatc ttttatttct gggtctaatt catatccaaa
     5341 agcttcgcat gaattttcaa ccatctttat accaccatat ggcatgacag ctctttttaa
     5401 aggggcatcc gtttgtaacc caactattgt ttctaattct ttatctaaat atcctggagc
     5461 atatgcagct attccagaaa cagtttttgt atcaacatct aaagtaccac cattttctct
     5521 ttctttttta aataggacca ttgcttcgtc ccataatttt ttagtatttt cagtggcacc
     5581 agctaagaaa gagtcatttc cctcatatgg agaatagttt aattgtataa actctctaac
     5641 atttatttct ttagtccatc ttcctgtttt aaatccttgc catgcattca ttaagtattt
     5701 cctccttaga tttaaatgat aaataatact attaaaatta atttttatat acttaattta
     5761 ttttatatat ttttttaaaa atatattttc taatataata atagcatatt attcgtatcc
     5821 tgtatacata aaaaataaaa aattgtcgct ttaaagtata aaacagatta tatgttaata
     5881 atgtggtgaa attataccca tatataaata aaataaaaca aataaatagc taattaagga
     5941 gaaaatattt tttatttttt gtttaggata aataaatttt attatggaaa aaagaaaaag
     6001 catacatctt cacaaactga atatttattt aataggggga ggtgatagat tgtttatcac
     6061 agtggtatta tttttagtag gtttcctatt aattactaaa ggcgcagata tctttataaa
     6121 ttgtactgta gagataggta aaaaaacgaa tatatcagaa attatacttg gagctactat
     6181 agttagtttt gcaacaacat tacctgaatt tacagtgtct ctacttgctt ctattgatgg
     6241 tcatactact atgagtttag gaaatgcagt tggttctata atatgtaata cagggttagc
     6301 attagggttg gttgttttta taagtccatt taatgtagat aaaaaaatgt ttttttctaa
     6361 gtctttacta ttaatagtat ccgttatagt tttaatttta ttgagtttag atggagttat
     6421 aacaagaggt gattcattat tacttataat aatattaatc ttttatatgg ttaataatta
     6481 tagaagtgtc gttggaaaat ctgatactaa aaatagaaat attaaaaata acactattaa
     6541 agacatatct actaaaaaag ggaaaaatta taggggtttt tccgtattag aaattgtaaa
     6601 aatactatta ctttttgcta caggattgat aatgatgata ataggttcac aaatccttat
     6661 agagagtgga gtaataatag ctagtttttt aaatatacct caaggtatag taagtttaac
     6721 tataatagca cttggaactt ctctgccaga gatagtttcg tcgattactg ctataagaaa
     6781 aaatcatcat gaaatatcag taggaaatat tttgggtgca aacatattga atatagtatc
     6841 agtaatagct gtatcagcaa taccaaataa cataccaata ctatctcaaa atagacaatt
     6901 ggatatacca ttcatgatat tattattatt aacagtaata ataccaacac ttaaatctaa
     6961 taaacttagt agaatacaag gtatattgat gttattcaca tattttctgt acatatcaat
     7021 tttgtatttc atgtatatta tttaactata ctgtttaaaa tattaattgc agaatatcct
     7081 aaaaaagtat ataatggata ggttaagatt aattgataac ggagaggaat aatatatgaa
     7141 tataacaaag tttgaagatt taccaataag tgagggaata aaaaaagcta tagctgaaat
     7201 ggggtttgaa gaaccatctc ctatacaagc acagtctata cctgcgattt tgtcaggtaa
     7261 agacgttata ggacaagctc aaactggtac gggaaaaaca gctgctttta gtatccctat
     7321 actagaaaca atagacccaa ataatagaag tttacaagct gtagtgcttt gtccaacaag
     7381 agaactggca atacaagtat caacagaaat aagaaagtta gctaagtatt cgcacggaat
     7441 aaagacatta cctatatatg gtggtcagcc aatagataga caaataaaat ctctaaagag
     7501 tggtgtgcaa gttgtaatag gtacaccagg acgtacaata gaccatatca atcgtaaaac
     7561 attaaagatg gataatgtaa aaatgattat attagatgaa gcagatgaaa tgctagatat
     7621 gggatttaga gaagatatag aaatgatttt aagtaagatt ccagaagaaa gacaaacaac
     7681 tttcttttca gctactatgc caagaggaat tttggaatta actaagagat atcaaaaaga
     7741 cccagagcat ataaaagttg ttagaaaaga acttacagtt tcaaatacaa agcaatacta
     7801 tatagaaact aggtcatcaa ataaattaga agtattatgt agattagtag atgtatatga
     7861 ccctaaacta tctgttgttt tctgtaatac taagagaaaa gcagatgaat tggtaggaga
     7921 tttacaagca agaggatatt ttgcagatgc attacatggt gacttaaaac agactcaaag
     7981 agatatagta atggataagt ttagaaatgg tacaatagat atacttgttg ctactgatgt
     8041 tgcagctaga ggtatagatg ttgatgatgt tgaatgtgta ttcaactatg acttaccaca
     8101 agatgaagag tattacgttc atagaattgg tagaactggt cgtgctggta gagaaggtat
     8161 gtcatttact tttgtatttg gaaaagaaat gagaaagatg aaagatatag aaagatatac
     8221 taaatctaaa ctaataaagc ataatatacc aacaataact gatgttgaag agaaaaaagt
     8281 aggtactttc tttgcacaag ttaaacaaac tatagaagaa ggtcacttaa ctaaacaatt
     8341 acaatggtta gaaggtttct gtaatgatga agattatgca atggttgata tagctgctgc
     8401 acttgtaaaa ctatctctag gtgaagaaat gaaagaagaa ataatagaag aaaaacctag
     8461 aagagagcgt ggagatagaa aaggtggtac tggagctaaa gatggtatga ttagattatt
     8521 tatcaatatt ggtagaaatc aaagagtaca agcaaaagat atagttggtg ccatagctgg
     8581 agaagttgga ataccaggta aagtagtagg tactatagat atatatgata agtatacttt
     8641 tgttgaaata cctaaaaaag atgcaaaaac agtaattgaa aagatgaaag atattaagat
     8701 aaaaggtaat aagattaata tagaaaaagc aaataagaag aaaaaataac gtatagtata
     8761 caaaactata attagaaaat atataaaata atatattgga tatttaaaaa gcctgatgtg
     8821 ttttcaaaac agatagtaga taatctgtaa gagttaacag cataaggctt ttcttgtttt
     8881 atataaggaa tctgaataac ttactgttaa atctttgaat tactgcacct catttacgtt
     8941 aaggatggag tattctacct atttttgata aaatatatct atgttttata tactgtagtt
     9001 ttgaatatat aaagtttaat aaattatagt atatatgtta atttttaata aggtaagaaa
     9061 gtagaaagaa ggatatttta tggatataat aaaaaaatta agtcatgggt gtacattaga
     9121 ttgtcatgac tgttgtaaat tcaatgttta tactaaagga aataatgtag taaaaattga
     9181 aggagataaa aatcatcctt atacaaaagg ttttatatgc aaaaaaggga tggcccattt
     9241 agatagatta aatcataaag atagaataaa gactcctatg cttaaagttg atggagtatg
     9301 gaaagagatt tcttttgata aagctataga aataatggca gaaaagctta catactataa
     9361 agaaaagtat acatcaaagt ctgtcatgca ttatgaccaa tatggaagtg ggtctgtgct
     9421 aaaatacata ggagatatat tttttaattt ttatggaggt gttagcagac ataaaggtgg
     9481 tccctgttgg agtgcaggga tgcatgctca aaaatatgat tttggagtag ctaagagtca
     9541 tgctatagaa gatatgttaa atagtaaaag tatatttgtg tggggaaaaa atccagcata
     9601 tactacaatt catactatgc aaataattaa aaaagctaaa gaaaaaggga taaagatagt
     9661 agtgattgac cccatatata caaaaacagc tcaaatagca gataagtatg tacaagtaaa
     9721 tcctggaaca gatggagctt tagctatagc tatggcaaaa attatagtag aagataaatt
     9781 atatgatgaa gaatatataa actcgtatgt tattggtttt gaagaatata aaaaatattt
     9841 atcttcattg gaattaagtt ttttaattga tgaatgtggt gttaaagaga atgatataag
     9901 agagttagtg gatttatata ctaacaaata ttcaagtata aatgttggat atgggcttca
     9961 aaaatataaa aatggtggaa acacaataag agcaatagat gctttaggtg ctataactgg
    10021 tcaaatagga tttagtggtg gaggggtaaa ctatgctaat aaagtttatc cgagtgttat
    10081 aaattctgac ccttacaata gccaatcata tggtgaagat agggagtttt atgtaagtaa
    10141 tatcagtaaa tttatagaag aatctttaaa aaatacatca aataaagtaa attatgcatc
    10201 agatgaactt gatatgacat caaataaagt aaattatgta tcagatgaac ttgatataac
    10261 atcaaataaa acagattata tatcaaatga attgtataat ttatctaata agtcaatcaa
    10321 aggtaatatt cctataaaga tggctgtaat aactaaaagt aatatgctga atcaactgcc
    10381 agatttagta gaattggaga gggtgttttc aaaaattgaa tttaaagtat gttttgatat
    10441 gtttataact gatacagcta ctttatgtga catgtttata ccgtgtacaa atacactaga
    10501 gagtgaagat ataatttata gctcaatgac caatccatat ataacctata atgaaagagc
    10561 tgtaaagcca gcacataaat tgatggatga atattacttc tttatggagc tagctaaaaa
    10621 gatgggatta aatgattatc cttttgtaga aaagagaact tatttagaaa aagtgattga
    10681 accacttaag cgttttgaca aaaatctgga tatagaaaaa cttaaaaaca attactttac
    10741 tatacataac cctgttgctt gggaagataa gaagtttgag actccatctg gaaaatacga
    10801 gctttattca gaaagtataa aaaacttggg aataagtcca acacctgtat atataagcaa
    10861 taaatataaa gaaatagaag ataaaaatat ttcttttaga ttattaacta atcatcatgc
    10921 agatacttta tttagtcagc attttatgga caaaaaatct atagctcaag catatataaa
    10981 tcggagaatg gctaaaaaag taggtattga ggacaaagat attgtgatat tgaggtcaaa
    11041 aaaagcaaaa ataaatgtac aaataaatat agatgatgga gtaggtaatt atatagtaaa
    11101 aatgtatgtt ggttggtgga agaaacatgg aaatcctaat agtttaacag acactggtat
    11161 atcagatttt gggggtcagg taacatataa tgaaagtatg gtagaaataa tacgacaaaa
    11221 ctagttaaat ttcattgaag gaataaaaat ataattatag aattgattaa aagttactat
    11281 ttttacaaaa ctattgaagg agggattttt gatggtaaat tattccttag aacataagaa
    11341 tctatacata gaatttatgt gctcagaact agaccaccat gtagccaatg aaataaggga
    11401 agaaatagat aacttattaa gtgtaaatca agtaaaaaat gttgtattta attttgaaaa
    11461 tattaatttt atggattctt caggtatagg cgtcatcata ggtagatata aaaagatttc
    11521 taatgaaggg ggacgagttt cagtaattaa tataagttca cgagttaaga agatttttga
    11581 tttatctggg ttaaataaaa ttattggcat ttatgatacg tatgaagaag ctctaagttc
    11641 tttgtaggga ggagtatgtg atgaataata ttatggaagt taaattttcg gcaatatctg
    11701 aaaatgaaag tttagcaaga gttatagtag catcctttgc agctaagtta gacccaacag
    11761 tagatgagtt agtagatata aaaactgctg tctctgaggc tgttacgaat gctataatac
    11821 atggatatga agaagacagt tctaagtttg ttttcttaag atgtgagata gaaggaaata
    11881 ctataaagat tatagttgaa gatgaagggt atggaataga aaatgtggag aaggcaatgg
    11941 aaccacttta tacatcaaaa ccagaacttg atagatcagg aatgggattc acagtaatga
    12001 aaagctttat ggacgatgta gaggtaagtt ctgtaaaaga taatgggact agaattgaaa
    12061 tgactaaaaa aataaatgtg cctaaataaa gtccaaaagt gaggttttat tatggaagta
    12121 actgttgcca gagaagaaaa aaaacctctt cttagtcatg aagagactct agaacttatt
    12181 gaaaaagttc aaaatggaga tgaagaggca aaagaaatat taatatcaag caatttaggt
    12241 ctagttagga gcgttgtatc tagattttta aatataggat atgatagaga agatttattt
    12301 cagttaggtt ctataggctt aataaaatca atatataaat ttgacccaaa atttaatgta
    12361 aaattctcta cttatgcagt accaatgatt ttaggtgaaa taaagagata cttaagagat
    12421 gatggtatga taaaagtatc aaggtcgtta aagcaaatag ctataaaggc aaagatggaa
    12481 agtgaagctt taactaaaaa attaggtaga gagccgagta tagaagaatt agctaaagca
    12541 atagatgtag aaaaagaaga tttagtaatg gctatggaag ctaatttcaa tgttgagtat
    12601 ttgcaaggag ttattcatga agaggaaggt tctccaatat gccttattga taaaataagc
    12661 atgaaaggag aaagtgaaga agaaaaagta gtggataaca tattgttaaa agacatactt
    12721 ggaagactgg ataagagaga gcgtcaaata atagttctca gatactttga agacatgact
    12781 caaagtgaaa taggagagat gctcaatata tcacaagttc aagtatctag gatagagaag
    12841 aaagtattgt ctaagttaaa agaatatata tcgtgataat acttatgata tgtagaataa
    12901 taaaatataa taaatatatt actaaaataa aatgtaataa ctttttatgt taagttttta
    12961 aattattaaa agatattaaa ataataaaaa gcattaaaaa agtaaaaata taaaaacaag
    13021 gtgtctttat ggaaaaatag attttttctt tattaggcat cttttttatt ttttgaccaa
    13081 actttagata gtcaaaataa ataataggtt tattttttta aaattaacaa ataatacttt
    13141 agagaggtgt ttagtatgga taaaaattat aaaaaatatg tagaccaaat aagcccaaaa
    13201 cctacatatt taaaaaacta tacacttgca tttattgtag gtggaattat ttgtatgata
    13261 gggcaagcaa taaatgattt gtatatgaaa gttggaggtc ttgataaact aggtgctagt
    13321 tctgcaacat caataacatt aatatttata ggggcatttt taactggatt aggagtttac
    13381 gacctaatag gaaaaagagc tggagctggt tctataattc caataacagg ttttgctaac
    13441 tcaatagtat ctccagcaat ggagtataaa agagaaggct atgtattagg tgtaggtgca
    13501 aacttattta aaattgcagg accagtttta gtttatggga taggttcttc tattttatgt
    13561 ggaattattt attatatttt taagatgttt tagatgattt ttatttaatt taaattttat
    13621 taattatgaa gaaggtataa aatctgaaaa tagctactaa agtaactaat tgaaaaataa
    13681 ttctgcagat ttaattaata tttttaatta atattaaaaa tttgaatagt aaaatgcaaa
    13741 attaaaaagg tgtgaaaata tgaaaaataa aagaattgga aaaagaacag tcaagttaga
    13801 aaataaacca accataatat caactggaac tatagtaggt cctaaggaag gtgaaggacc
    13861 actaaaggac tattttgata tgattatgac tgacgattta tatggtgaaa agacttggga
    13921 attagctgaa agtaaaatgg tagagactgc atctcaacaa gctatccaaa aggcaggtaa
    13981 aaaattgtca gatgtaaatt atatgctagg tggcgattta ataaatcaaa tagttccagc
    14041 atcctttgca gcaagagaac tggctattcc atttttaggt atatatggag cttgttctac
    14101 aatggcagaa ggtctttgta taggttctat gcttatagat ggtggttttg ctgatttagt
    14161 actgtcaggt acatcaagtc actattgtac agcagaaaga caatttagat tcccacttga
    14221 acttggaaat caaaaaccaa tgacagccca gtggacagtt acaggagcag gaagtgtatt
    14281 attagctcct aatggagacg gtccaaaagt aaagtatgtt actgttggta aagtaataga
    14341 tgagggtatt gatgatggta ataatatggg accagctatg gctccagcag caatagatac
    14401 tatctactct tactttgaag atactaatga tgacccaaat agctttgata taattgctac
    14461 tggagattta ggtactcttg gaaaacagat agcagaagac tttctaaaag aaaaaggtgt
    14521 agatatatca aaagtttata ctgactgtgg tatagagatg tttaacctta aagaacaaga
    14581 tgtccactgt ggtggaagtg gatgtgggtg ttcagctaca gtgtttgcag gatatatata
    14641 tgataagttg agaaaaaaag aatttaacaa agtaatgctt gtatcaacag gagcactact
    14701 ttcaccgaca agtacactac aaaagcagac aataccatgt gttgcacatg cagtagtaat
    14761 agtaaatgag taggaagtga tataatatgt taatggacta tgtaagagta tttattgtag
    14821 gtggaattat atgtttaata gcccaagtaa tgatggatta tttcaaaatg caaactccat
    14881 atgttatggt aacgtatgta acaactggtg tagttttaac ttttctagga ctttatgagc
    14941 cattagttaa gtttgcagca gcaggtgcaa cagttcctat aataggattt ggatattcac
    15001 ttgctacagg agtaataaaa tcaattcaaa gtgatggttt tttaggtatt tttttaggag
    15061 gtacaactgc tacagctggt ggaatagcag cagctatatt ctttggatat attatgtcag
    15121 tagtatttac tccaaaagca aaaccataaa tctttattaa aagaataata agtttttgaa
    15181 ataataactt tttataaagc aggtggatat cttttagatt ccacctgttt tttatataaa
    15241 gaagcgttta cataattgaa gttgacaatt atttacaaca tagtgtagta ttatattatc
    15301 aaaataaaat gaagtgaagt aggggataaa gatggtaact attaaagata ttgcaaggaa
    15361 tctaggtatc agttattcaa ctgtttctag gtgcttaaac aataatccta atgtatctga
    15421 aaaaacaaaa aagaaagtgg tagaagaggc aaatagacta gggttccatt ttaatgtaaa
    15481 tgcaagaaat ttagcaaaaa aagaaactaa caggataggg gtaatctttt caaataattt
    15541 taatcatcaa gatactagaa agttttttag tgatataatg gatagttcta taaattctat
    15601 tgagactaat aaatacgatt ttataataca gcctaataat aacattagtg gtgatagtaa
    15661 tgtatataaa atggtaaatg gacaaatggt tgacggatta gtaattgtgt ctcaatcaat
    15721 aaaaaaagaa gagtatgatt ttttaaaaga caataatttt ccacatgtat ttatatattt
    15781 taaaccatct tttataggag aagtagataa tttcttttgg gacgataacg tgtatggagg
    15841 ttatatagct accaagcatt taattgacca tggtcataaa gatataataa cagtaacttc
    15901 agatgataag agttcaaaaa tgcatgaaga tagaaccaag ggatatttga atgctatgga
    15961 agaagcaaat ttaaagacag aagtcattaa atctaaaatg gattttgaat ctcaagttga
    16021 atttttagaa aaatacattg ataaaataaa aaaagcttca gctatatttg tacaacaaga
    16081 tgtaccagcg atttctataa tacaagagtt aaaaactact tatggtataa atgtaccaga
    16141 agatatttcc ataatagggt ataataatat tgaattaata tcatatttta ggtcgcattt
    16201 atcaacaata gatgacccaa gagagcaagt tataaaaaat ggagtagata gtttagtaaa
    16261 tataataaat aaaaagagta cagaacacat tccaagaaaa ttatatccaa ggttaataat
    16321 acgaaatagc gttaaaagaa taaagtagta agcttttaag tatcaaacgt ttattaaaaa
    16381 tattatagat tatgtgaagt tttttaacaa ataatataaa taaaaattaa aaggagtagg
    16441 tgtatataag tacattgtac tcctttattt attgagatag tttttataat atgaatgaat
    16501 aattttattc aattatttga aaatccgtta ttttaacaat ataaattaat aaattatttg
    16561 tattttatat catgaatcta taatattttt ttgattcata actaaaagta tataaaaatg
    16621 atgaataaat atatattata cattatcaat gataggttat ataataaaat cactaacaat
    16681 tatattataa gaaaattata aaaattacaa ggaggtatag aaatgaaaga aaaagcaggg
    16741 attagaaatg ccataatagc agcctttata atcacaatat tggcagaatt aattggacct
    16801 atttcattta aagttatggg aattaatgta actttactta gtatactatg ggctatattc
    16861 ataggaatgg ctgtttctcc tcatttatta gggagagtta taccagcctt aaaaaagttt
    16921 ataggggata atgaaataaa tgtatctcca tatttattat ctttgacact atatccatta
    16981 gggataatgt ttggaattaa tgcagggcct aaagttggta tattacttca agcaggacca
    17041 gcacttttat tacaagaatt tggtcatatg ggtacaatgt tgatagcatt acctgttgga
    17101 gtagcattag gtcttggaag aagtgcatta ggtggtactt tttcattatg tcgtgacaca
    17161 gcactaggaa ttattggaga taagtatgga ttaaactcac cagaaggtat aggaacacta
    17221 ggaacttaca taagtggtag tatatttgga acattattat tttcattttt agcaccagtg
    17281 ggtgtacttt taggatttca tccatatgct ttagcaatgg catcaggaat gggtagtggt
    17341 tcaatgatgg gagctgcaac agcatcatta atgaatacag ttccacaaat gtcagagcag
    17401 attttagcat attcagctac tagtggattg attacagctg ttactggaat atatatagaa
    17461 ttattcttat ctttaccagt agccaactac tattatagca aagtagctcc agttattgaa
    17521 agatttagag gaagaaaaac aagaagaaca agtaataaat tgtaaaggag gatatatata
    17581 tggaaaaaac ttttaagact attatggagc agataaaaat agtattgttt atatatatta
    17641 ttatttgctt tggacaaatg atagcactta agactcaggc tcaagatttg ttgattggaa
    17701 gtgtatctgg atttttgttg gtgataattg ctattataat aaagaatttt gttaaaaaac
    17761 ctaatttacc aggttttgca tgggctacat tagttgcatt tgttttatcg cttccaatat
    17821 ctccagtggg ggatatcata gcaacaaatg tagggaaaat caattttatg tctacagtaa
    17881 caccattatt agcttttgca ggaatctctg taggtaatca attagatata ttaaagactt
    17941 tgagttggaa gttagtagtt atatcattta tagttatgac atctacttac tttggttctg
    18001 cttttatatc tcaaacagtt ttaagattaa atggaatgct ttaaggaggt agtaaatatg
    18061 aatgaattaa aagcaaaaat taaagaggaa ataaagttat tatctgaaga gaaaaagaca
    18121 tcatttgaaa aagtttctga ctatttattt tcaaatccag aattagcatt tgaggagtat
    18181 aagtcacaaa aagcattatg tgatttgtta gaagaaaatg aatttaatgt aactaaaggt
    18241 gtaggaggat tagaaacatc ttttgaagct gtgtattcaa atggaactaa tgggaaaaca
    18301 gttgcttttt tagctgaata tgatgcactt ccaggaatgg gacatgcatg tgggcacaat
    18361 atcataggga caagttctgt tggtgcaggt atcatattaa aagaaatcat gaaaaaacat
    18421 aatatagaag ggactgtaaa ggtttttgga acacctgcag aagaaagagt aggtggaaaa
    18481 ataactatga ttaaagaagg tgtatttaat aatgtagatg ctgcattaat tttacatcca
    18541 agtgatgctt caatgcctga tgatatatca tttgctcaag ttaatttaaa atttgatttt
    18601 actggtaagg catcacatgc tgctgctttt ccttgggaag ggaaaagtgc cttaagtgga
    18661 gtaatagcac tatttaacag tgtaaatagc atgagattac atcttaaaga ttatgcaaga
    18721 gtacatggaa ttataactga tggaggtagc atacataata taattcctga aaaatctaca
    18781 gctatattta atgtaagagc cttaagtata gaatatttaa atgagatttg tgaaatgctt
    18841 aaaaattgtg ctaaaggagc tgcaatttct actggaacta atgtagaaat agttcaatta
    18901 gatgaaatat acaaagagat taagaatgat tctgaattag tgaatatagt tagacaaaat
    18961 tttgaagttt tgggtgaaga ctatgttgaa agagatttaa gtcaaggtat aggttcaact
    19021 gatacaggaa atttaactca tgagatacct gcaatacaag catatataaa attaaaagaa
    19081 aatactgcaa ctcatacaga tgaatttgct gttgcagctg gtggtgaaga aggcagagta
    19141 gcacttataa aagcaattaa ggtgttagca atgtgtggag tagatatttt gtatagtaaa
    19201 taatatataa ttttaaaata tattaagaaa ggaaatcgga ttagacatga tattaaaaca
    19261 agtaatagag gtttatgaca ttttagataa agcaaatgct aatggggaag aagtaaaaac
    19321 ttacttacaa ggttatggtg aagtagatgt aactgtaaaa gaattgtctt caagtaaagg
    19381 ttcaacagat ttagtaaagc ttacaattcc tggaaaaaat ggaaaattaa aaggtggaga
    19441 tgctcctact cttggtattt taggtagact aggagggatt ggagcaagac cagaagttat
    19501 aggttttgtc tcagatggtg atggagcatt ggtagcaatt gcagtggctg ctaaactttt
    19561 ggatatgcaa cgaaaagggg atatattaga tggtgatgtt gtaatttcaa ctcatatttg
    19621 cccagatgct ccaactaaag aacactatcc tactcctttt atggattctc caattgatat
    19681 gatgactatg aatgaaaatg aagttgatag tagttgtgat gcaatacttt ctatagatac
    19741 tactaaagga aatagagtaa tcaatactag aggatttgca atatcgccaa ctgttaaaga
    19801 aggctacata ttaaaaacta gtgataacct tttagatatt atgcagactg taacagggaa
    19861 atttccattt gtattccctt tgtctattca agatataaca ccatatggaa ataatctgta
    19921 tcacttaaat agtattttac aaccagcagt agctacagat gcaccaatag taggagtagc
    19981 aattacaaca gagatgcctg ttgctggatg tgctacaggt gcaactaatt ttagtgattt
    20041 agattcagct gggagatttg caattgaagt tgccaagcta tttggtagaa ataagtgtga
    20101 tttctatgat aaagaagagt gggaaatgct tattaagaga tatggcaaat taaatagatt
    20161 tcaaacattt ggtatacagt aattagaaaa atattaagtt tatataattt gtgatataac
    20221 tatttaagaa tatataaaca attaggaata aaagtaagaa taaagcactt agttaaaaac
    20281 tttaaaaata aaatatttat ttgtttatat aaaaaggagg attctggtta ttgggattct
    20341 cttttttatg aatacaaata atatattgta attataaaaa aaggagcgtt tcaaagtgat
    20401 ttttttaatc accttgagac aactccttat atttttattt aaattctatt ttttattaag
    20461 ttgctggagt tccaccacca tcaggaggag tagtagagcc accaccatta tttccaccac
    20521 catcaggagg agtagtagag ccaccaccat tgtttccgcc attatcagga ggagtagtag
    20581 agccaccacc attatcagga ggagtagtag agccaccacc attgtttccg ccattatcag
    20641 gaggtgtaga accgccacca ttatcacctg tgttattatt gttattatta ttttcttctg
    20701 gttggttatt gtctggtttt tttgtatctt cagatgatgg ttgagaactt actttagtag
    20761 gagcagtacc ttcaataaat gctgctttag agccagaaga ttgctttcct ccatcctcta
    20821 aattaatttt tgtaaaatat acatttttag gaacttcaaa ttcagtaacc gttaaattag
    20881 catgtatttt ttccataatt ttttcccata gttttgcagt actagtactt ccatgtggta
    20941 cttctctacg ttttatagtg ttaccagaat catcttttct accagcatca tctccaatat
    21001 aagttgcacc aacataataa ggagtgtaac cgacaaacca agcatcatat gcactatttg
    21061 tagtacctgt tttacctgct acaggcattc cctttggaag tgtagcaaca ccaccagtac
    21121 cttcagtaat tacgctttct aacatatctg taacaacata agctacttca gggtctacaa
    21181 ctttatgttc ttcaggagca tttttaacta ataattgacc atcaaaggta gttatagtag
    21241 taaaaataga aggttctaca taaataccgt gatttgccaa tgtaccataa gcagcagcca
    21301 tttgaagtgg agtaatacca ttcgccatac cacctagtgt caatgatggg aattttctat
    21361 cacttgcttc agatgaacct gagtgattat tctttaaagt tgttatacca aagttcttta
    21421 agtaatccat cattatatct acacattcat cataagagtc tccaagcatt tcagcagttt
    21481 tgacagctat cgtgtttgaa gatttagcaa gtgctttacg taaactcata gaaccatgac
    21541 cagcagtagt agttcttgga ttccatttat tattttcttc aaacttatat cctcctcgag
    21601 aatcacttag agcagttgct tgtgttattt ttagagtatc aatagctggc gtatatattg
    21661 ctaaaggttt tatagaagaa cctggctgat gagggttagt agcacgattt aaagtttttc
    21721 taccacttat atttcttcct ccagctagtg ctcttatttc accattttta taatctaaga
    21781 ttaccattgc agcttgcggt tggctaactc catcaggacc gaccatatgt cctgggaaat
    21841 tgctatttct atcaaattct tcttctaaag catcttgcat tttagggtct atagttgtgt
    21901 ttacaattag accaccattg tagaataagt tttgagcttc atcatttgta tgtccttgtt
    21961 ccattaaagc atttataact tcactttcga taaggtcttc tacagatgaa gaaactttat
    22021 ctgatgattg aggcaatttt atttctattt tagctttgat tgcttcatca tattcacttt
    22081 gttttatata tttcaattct aacattttct ttaaaacaat ttcttgtctt tttttagctt
    22141 caggattact tacagccttt ctaacaacta atgtacctgc ttttaattgt ctataagtat
    22201 cattagatat aagctcccaa gattttattt tttcaatcat atcaaagtcc acttgagtag
    22261 ggtcatctaa atcatcatca gttgtgttaa caaaaaataa taatttattc tctaaatcct
    22321 ccttagtttc atttccctct aacttagaag ttttataggc tgaaaatctt gatggatttt
    22381 tagttgaacc agcaagaagt gctgactctg ctagagtaag gtcagctgca gacttatcaa
    22441 aataaccacg tgcaccagcc tcagcacctg ctagacctct accaacgaag aagttattta
    22501 aataagtttc tagtatttta tctttactta ctgttttact catctcatgt gcatagtaaa
    22561 tatcctttat tttacgaggt atagtttgct gttcagttgt taacagcatt ttggatacct
    22621 gcataggaat agtactacca ccttgcttag tacctgtaag ggtctttaat actgacctaa
    22681 ataaaccttt aatatcgaca cccttatgct cataaaaacg ctcatcttct attgcaatta
    22741 cagcatgttg aagataaggg gaaatcttat ctaatggaac gggtgtcttt ttatttacac
    22801 ttggagctgt agataatgtt tttccattag cgtatttaat agtagttgtt tggttcgtct
    22861 gtttatctaa aacagcttct gtaactggag ttacatctct tagtgacgca aatactagac
    22921 cagcagtacc tgctgtacca acaactagta aaactaaaaa tactatccca aatactctta
    22981 gttttttaaa tttatcgctt tttttagatt ttttcttgtt ttttgttgaa cgagtcttgt
    23041 tagctgaagt tctattaatt ggtttatttg tattaccaga tgagctaact ttttttctcc
    23101 tgattttatt tccattttta tcattatcgt tattcataag attttaacct ccttacttaa
    23161 tgcaatatta taacacaaat aggagtagtt ttgtaattaa tagaaaaaat gaatttgcta
    23221 taatattctg tacattgtat atgtataaac gtaatatatt aaaaagtcta tatttttatt
    23281 ttatttattt ttgacaaata ttataaaaat tatgccaaag ctacatataa ataaatagga
    23341 ggggataatt ttgaatagaa tagtagatag aaaaacaaaa aatgaattta gaaaaatcat
    23401 agctattttt ttagtaattc ttttcttgtc tgtatttata ggcagtttta tatatataga
    23461 taaaacttta agaccgacaa taacagtttt agcagagact aaagctcttg aactagcaaa
    23521 taggtcgata aataaagctg ttgctgagat ggtagaagga aaaataaatt atgaagattt
    23581 aatggatata caacttgata ataatggtaa aataactatg atacaagcaa acactataat
    23641 gatgaatgaa attgcatctg caatagcttt agaaatacaa gatgaattaa aaaaggataa
    23701 gacagcaagt tcttacatac ctataggaac agcattagga agtcctatat tagctaaata
    23761 cggtcctaaa cttgaagtat ctattgaacc tataggaaca gtatcagtaa actttaaaac
    23821 ggaatttgag tcttcaggta taaatcaaac tagacataga atatatttag aagctcaaac
    23881 acaagttaaa gtagtaatac cacttataac ttcaacaaaa caaattaaag ctcaaatacc
    23941 tatttgtgaa actataatag taggtgatgt tccagaaagt tatgtaaata ttccagaaaa
    24001 aaatctagga aatgttttac ctaacactgg aaagaatacc aacaagtaat gtaaagttgt
    24061 atatttttaa ataaattcaa aataatagtt tataaataaa atttttggaa ataatattaa
    24121 tataatttta ataaaaattt atattttata ctatgtaaaa tatctaatat atttggtctg
    24181 taggttaaat aaaagtacct ttagaccaaa tatattatta ataacttaat aacatcttaa
    24241 aacttattta tttatattat ttacaacatc catttaataa atacaccata acctacagct
    24301 gggtcgtttt caattgcatg tgacactgct ccgattattt tattatcttg aactattgga
    24361 gtaccactca taccttgtac aataccacca gtttgagcta aaagcttttt atctgtaatt
    24421 ttaattttaa aacttttact ttctggatgt ttttggtttt ctatatctat aatttcaata
    24481 ttatattttt tacacttatt aaagttatct tgtaaaatta tttgagcttt tcctaattta
    24541 acttcatcta aagatgctac tttatatttt tttaacttgg aaatatcaaa gttattaatt
    24601 tgacctctta ttccaaaatc agtattttct gtaaagctac caataatatt ttctcgtttt
    24661 gcatttatac atccaacact acctttataa gatttttcta tattaaggtg cttagttgta
    24721 gaaattgaac ctgttttaat aggtattttt cttgcattac caacacttat tggatgacct
    24781 acagctccaa actctgatgt atctggattg atatacgtta aagtagcaaa tccagaaagt
    24841 aaattgttaa aactaatttt ttctaatatg tgtttggtag ttttcattgt tataagttgt
    24901 ccacctctat ttaataaaat agatacttgt ttttcttctg gtaaagcatt taaggtattg
    24961 gcaaagtcac tatatccatc aacttcagca ttatttacct ttactatagc atcacccaat
    25021 ctaaatggag atgcttctcc aggatttcta actatgatat tttttaattc tgcgtcaatt
    25081 tgaagtacgt ttccaattgg tattaaatat tcaggttcat ttttttgtaa attttgtgca
    25141 taaattaaat tatttgagaa aaaatataat aaaaataaaa atgttaaaac aattgtaaat
    25201 tttgacttag ctaagttggt ttttttactt aaagtattat ttttaaaatg aaaattttta
    25261 agttgcataa aacacctctt tcttattaaa ttattttaat aatagaattg aagttttctt
    25321 gctgcttcta tattatgttg tgcaagaatc atgttttgat gcttggaaaa ctagctcaat
    25381 aacaccttag ttttcataaa aataaataaa ataaatagtg tatatgataa aataaataca
    25441 tattcatata aataaaaata aacaataact gtctagcata agaaaggagc aaaaaatgaa
    25501 aaaatataga ttgttagtta cttgtataat tgccatggga atcataatta taggaacatc
    25561 aaatccagtt agcaattttt acaagactac aaatttgatt agttcaggaa atatcacaaa
    25621 tgaaaaagca gataaagatg aaaattcaaa caaaaataat aaagataaaa atgaagacaa
    25681 atctaattct aaaagtaata aagataagtc atcagaggac aaatcgtcag ataataaaaa
    25741 tgaaattcaa aaaaataaaa aacttttaat ttgtatagac ccagggcatc aaggaaaagg
    25801 agatagcaat ttagaaccag tagctccagg ttcttcctct aaaaaagcga gagtatcttc
    25861 aggaacagag ggtattgcta caaaaaagcc agaatatgtt ttaaacttag aagcatctct
    25921 tgttttaaag agtatattag aatctaaagg ttataatgta ataatgacta gagagacaca
    25981 tgatgtaaat ataagtaatt cagaaagagc aatacttgct aatgacaaaa aagcagatat
    26041 ggttgtaaga atacatgcag atagtttaaa taattcttca aaaactggag catctatatt
    26101 gattcctgaa aaagatggaa aatatacagc tcctatatat gaagaaagta ataaatgtgc
    26161 tgaatttatc aaacagaata tggaacaatc aggtatacaa atcaatggaa tcgttcaaag
    26221 aggtgatttg actggattca actggtcaaa agtaccagct gtattagtag aaatgggatt
    26281 tatgagtaac tataatgaag accaaatgat gtcaaatcca gactaccaaa gaaaaatgat
    26341 gcagtgtata gctgatggat tggatgcgta ttttaaataa gatatgaata gagaaaaagt
    26401 taggttggta ttatttgcct aatttttatc tctatttttt attgtacata acacaaagaa
    26461 gaagtttatt ttaggttaaa ttgtcgtaaa tataactaaa aatattggga atacttgatt
    26521 taagtttaaa aagaatatat tatagtatag aatgtgtaaa atttttttga gaggtgttga
    26581 aatgctagta gtagaaaatg tgagtcatgg atttggtgcg agaacaatat tagaaaatgt
    26641 atcctttaga cttagaaaag gtgaacatat tgcactagtt ggtgctaatg gagaaggaaa
    26701 atcgagtttt ttaaatataa taacaaagaa gcttatgcca gatgctggaa atataaaatg
    26761 gtcttcaaga gctacagtag gttacttaga ccaacatacg gtattaagca agggaaaaac
    26821 tataagagaa gtgcttagag aggcttttaa gcatatgttt gacttagagc aagaaatgat
    26881 tgctatgtat gacaaaatgg gagaagctag tgatgatgaa atgagtaagc ttcttgaaga
    26941 aacagcagag atacaaacta tacttgaaaa tagtggtttt tatatgatag atgcaaagat
    27001 acaagaagtt gcaaatggtc ttggtcttgg agaaattggt cttgataaag atgtaactga
    27061 cttaagtgga gggcaaagaa caaaagtact tcttactaaa cttctacttg aaaatccaac
    27121 tattcttata ttggatgagc ctacaaacta tttagacgaa gagcatataa catggcttac
    27181 taagtacctt caagagtatg agaatagttt tgtcttagtt tctcatgata ttgaatttat
    27241 aaataataca tgtaatgtaa tatatcatat ggaaaatgga gagttaaata gatataaagg
    27301 aaattatgat gaatttgtaa gacttaatga tataaagaaa cgtcaagaag aacaagctta
    27361 tgataaacaa gttgaagaaa gaaaaagact agaggatttt gtagctagaa ataaagctag
    27421 agtagcaact agaggaatgg ctaacagtag gcaaaagcaa cttgataaga tggaaatatt
    27481 agaaagacca aaagaaaaaa taaaacctac ttttgctttt aaagatgcta gagcagctag
    27541 taaaataatt tttgaaacag aaaatttagt tttagggtat gatgaagcat tgacaaaacc
    27601 acttaatttc catttagaaa gaggaaaaaa gattgcctta aaaggaatga atggtattgg
    27661 aaaatctacc ctattaaaaa ctttacttgg aataataaaa ccatttgaag gaaatgttaa
    27721 attaggtgac tatttggaag ttggatattt tgaacaagag agttcaagag aaaatagtaa
    27781 tacacctatg gatgaagttt ggagtgaatt tccagggctt acaaactttg aagttagaca
    27841 ggctcttgca aaatgtggac ttacaaatga acatataacg agtcaaatga gagtattaag
    27901 tggtggagaa gctgctaagg ttagactttg caaagtaatg ttaaaaaata taaatttctt
    27961 agtattagat gagcctacaa atcatttaga tgttgaggca aaagatgaat tgaagaaggc
    28021 tatcaaagag tttaagggaa cagttctttt agtatgtcat gagccagaat tttattcaga
    28081 aattgttgat gatgtatgga atatagaaga ttttacaact aaaatcgtgt agtgatttaa
    28141 cttattgctg gggggatttc atgaaaaagt ttctaaatgt acttttttca atttgtatta
    28201 gtattgttat aatagtaggt gttattaatt ttactgttgg atttaaacaa ctttattatt
    28261 ttgacattga ttacttaaat atatcagagt tatctggttt atctaaagat gatataaaat
    28321 tgaactacga ttatctaatt gattataact taaataagaa tgtgtctgag ttcaaattac
    28381 caacattaaa atcttctcca caagggaaga tacattttga agaagtcagg aatatatttc
    28441 aaaatataaa taaattagct aaattattat tggtagtttc tttagtagga attatattga
    28501 gtgtaaaaaa taaaaatata aaaatcttaa aaactacatc aataacactt ataattatgc
    28561 cattattatt aacagttcca attttattaa attttgaaaa gtcatttatt atttttcata
    28621 aattattatt tagaaatgat tattggatat ttaatcccga tttagaccca gttataaata
    28681 tattgccaga ggaatttttt tttcactctg gaatgatgat tttaatatta attttgttag
    28741 taagtatagt actgtttgta atgtataaat tatgtaaatc tttatcaata aaaatatttc
    28801 aatgttaaat agagggatat ttttatttcc aaaataagat aataaaaaag gattagatta
    28861 aaagttatac aataaaaata gctgtgcaat aatatttaga ctatgaaagt agttgaaaaa
    28921 tatttatgca cagctattta taatagaatt tactttttac tagtgatttt aactaaatct
    28981 tgaaatttac tcataatgta attgtaatta tcttttttat gaggtaatat acaactactg
    29041 caatctttga tacctttatc tgtataccta aagtttccac cacagttttc tcctaaagca
    29101 tacaaaggac aatagcaaaa tagacagtta aactcttctg gtttattagt tttatgacaa
    29161 gggaaaaatt cgcaatcttt gtgattaaaa aacttataat tttcgcccat attttcctcc
    29221 taattaaata aaatttaaat tttttatact atataagatt ttggtgataa tataattata
    29281 ctaaataatt ttatgaatag tcgataatat agtattatta atcatagtat taatggtata
    29341 attagtcaac agaaaaataa tgtaaaaata atgtaagtgt attttgtaat tagaatatgt
    29401 tttataacaa aaaatgaggt gattaagtga aagaaattca agaaaaagca cttcttgtag
    29461 gtctgaattt gactacaata gtgaaaaaaa atgatgatat agacactaat gaatctatgg
    29521 aagaattaaa agaactaaca aaagctgctg gggcagaagt tgtaggtagc ttaattcaaa
    29581 ataaacactc tgtagatgca gcttattata taggaaaagg taaagtagaa gaaataagag
    29641 cctatagtga ctctttagat gctactttag taatttttaa tgatgaatta tctggtgctc
    29701 aaatcagaaa tatagagaat gttgttggaa gaaaagtaat agatagaact actttaattt
    29761 tagatatatt tgctcaaagg gcacttagca aagaaggtaa attacaagtt gaattagcac
    29821 aattaaaata tagattgcca cgactttatg gaatgggtgg tgagatgagt agaacaggtg
    29881 cagggattgg tactagaggt cctggagaac aaaaactaga aatagataag agacatatac
    29941 ttaataaagc agcagatata agacgtgaac ttaaagaagt aaaaaagaat agagagacac
    30001 agagagttaa gcgtttaaag tcaaatatac cgattgtagc acttgttggg tatacaaatg
    30061 caggaaaatc taccctgttg aacgaattaa taaagacaca caaggattat gaacaagaaa
    30121 aagaagtttt tgtaaaagat atgctttttg caactttaga tgtaactttg agaaaggctc
    30181 ttttgccgaa taaaaaagaa tttttagtag tagatactgt tggatttgta agtaaacttc
    30241 cccatgattt agtagaagcg tttaaggcaa cattggaaga agtacagtat gcaaatttaa
    30301 tcttacatgt aattgatgca acaaatacta gttatgaatt gcaaaaaagt acgactgaag
    30361 gtgtattaaa agaacttggt gtaaatgata aaaaacatat acttgtatat aataaagtgg
    30421 acaagctaga attggatata taccctaaaa gtcaagaaga tattgtgtat atttctgcaa
    30481 aacaaggcat aaatatggat aagttattaa atatgataga aattgcttta atggaaaata
    30541 cttattcagt aagtttgatg ttaccttatg agagaggtga tatatttagt agaataaaag
    30601 ataaatataa tgtggagaat tttgagtatg gggaaagtgg tataacgcta gatgcagatt
    30661 tagatgaaga agattttaat atctatagag agtacatact agaaaaatag gttgaatatg
    30721 gatataaact gggaaaatat agataatctt gaagattatt ttataaccta tcttctttat
    30781 aaagaatcta aaacagtatc tcaaattagt aaaattagaa atatttcaag tactgaagca
    30841 agtgagcaac ttatacaagc aaagttaaaa ataaaggaaa tgcaaaaaga tgattttgag
    30901 gcatctaagg atattttgga taaatttcta gaactagata aaattaagag acttgatttt
    30961 atggatagct tagatgacga aaaaatggta tactttaaga gaaaggtttt caaaagaatc
    31021 cttgtagaaa aaaatgctga agatttaatt gttttaattt gggcgacagg tgagctaaaa
    31081 gatgataggt ttttgaaatt gcttcatcag ctcacaaacc acagacactc agatattaga
    31141 agaataactt attcagcaat cagaaagatt gaatcaccaa gtagtagaga agtactgcaa
    31201 aaaggtctct acgacaaaaa tgcacagact aggcagtact gtgcaaaagc tctttcaaaa
    31261 ttgggagatg aaaacagttt aaagatttta cagcagttga aagacaagaa taagaatttt
    31321 gaaaaagaat atgtgcttag agcttatgac gaagccatta agtcattgga aaatgcaaaa
    31381 aatttgaaaa agtaattttt tattaaagca atttgtttac ttaatgatgt atttctaaag
    31441 tttaacaagg taataaataa attaatggga ggggtttcaa atgatagtaa gacgttgtgc
    31501 aggtggagta gtgttttatg ctaataaagt acttattgta aaaaatgata ggggtgaatg
    31561 gacacttcca aaaggaaaga tactaggagg gggacttcca tatgagagtg ctgttcaaag
    31621 agttaaagta gaaactggca tagatgctaa aatgatagat gtggctggcg atactatgta
    31681 tgaatttttc tcaagaagta gacaacaaga agtttgtaat gcaataatgt ggtatgttat
    31741 ggaagcttgt aacactgatt gtgtacttgc tcctgaattt caagaaggtg gattttataa
    31801 agtaaaggat gctttagaaa tgttatcaca tcataaagag caagctttag tagaagtatc
    31861 ttataaaaag tttaaggaat taaagaaatt agtttcagag ggcgatgatg ttccagcaaa
    31921 ttaattgtga atttagagga ttctgaggaa gggatagccc cttccttttt taattgaagc
    31981 agttttaagc gtaaattata tagtctcttt aaactatgat ttatcaattt agagatttag
    32041 tagtttatat ttaaaatcag taagatatat ttaaaataaa aattaaaaag tatacaattt
    32101 tattatataa ttgtcttatg atgttgatta aagaagtatt aaaagtccat ttatttttaa
    32161 atagaatgat tttataaata tatatttgat gtgtatatta gaaaatgatt aaaataggag
    32221 gaaatacaat gagcaattac aggacattac atgaatttgg aacggatgaa ataacgattg
    32281 aaaagtctgt ttttattgga tatgctaagc ctataaagag tgaagaagaa gcattagagt
    32341 ttataaatga aataaagaaa aagcataaag atgcaactca taatgtgtgg gcttatactg
    32401 taggtaaaaa tatgaatata caaagatata gtgatgatgg agaaccacaa ggaacagctg
    32461 ggatacctac attagaggtt ataaaaaaag aagatttgag agatgtagcg gttgttgtta
    32521 ctaggtattt tggaggaaca aaattaggtg caggtggtct agttagagct tatacaaaag
    32581 gtgctaaact gggacttgaa gcaggtaaga taatttataa ggtaatgtat caagaagtta
    32641 aggttaagat agattatact caacttggaa aagtgcaaaa tgagttaatg aatttggggt
    32701 attttataaa agatactgta tatgaggata atgttgaaat agtagtatat tcaagattag
    32761 aagatgtaga gaaattatca gaaaaaatga ttgatataac aagtggaaca ggaaaaatag
    32821 ttttaggaga ggaattttat ttgtctgaac aaaatggaga aattttaata taatctaaaa
    32881 ggttattaga atagttaata cctcaaatag ccaattagta tttttaagtg aaaagccatt
    32941 aaattgattt aatctaaatc aatttaatgg ctttttatcg gattttgcac agaatccttt
    33001 ttttacttca aataaaccga ctaaaagtaa aaatatccca aatatttttt ttaatatatc
    33061 tgaagacaga taattagcta atagtgaacc aaatacagct cctaaaaccc caaacacagc
    33121 tataggtata accaatttaa aaattacatt tttatttttt atatgtatag caagtgctat
    33181 aagtgtcata gggatagatg aaagtaaatt tacgctttgt gctattttag tgtctatgcc
    33241 agcaagtaaa acgagtgcag gtataaggat tgttcctcca cccattccca ttcctccaat
    33301 aattcctgca aaaaatccaa ttagagcaaa taacattaaa acaccatcct taatgctgca
    33361 attatcataa ttattccaaa agaaattcta agaaactttc cagtaacctt atttagtaat
    33421 ttagctccta taataccacc aagaatactt ccaactgcaa ctttaattgt taagttaaca
    33481 tcataagtac ctttagatac atataaaaca gaacttgctg tcgttaagaa tattattata
    33541 gaaagagcag ttgcatgaga tttatgttct tctaccttaa cgatgtcatt taatattgga
    33601 actaaaagag tacctcctcc agaaccaaaa actccattta taaatcctgt aaaaactcca
    33661 attatcgtat tttttaaatt aaaatcaaag tattttttct ttaaatcatc agtttttttc
    33721 ataaaaatca ccttctgaaa tagttttacc aaaataggca aattatattt atataaaata
    33781 agattaaata taatctttca tgtttgttta attttgcata aaaaaaaaca tattaaaaca
    33841 acatattaat atatggtata atatattaaa acaaagtagc aaatttgaga ggagataaca
    33901 atgtcaaata taaaaataca aatagacaaa actgtagaat ggcttataaa taaggttaat
    33961 gaggcaaatg caaaaggatt aattgttggt gtatctggag gaattgattc ggcagtagtt
    34021 gctaatttaa ttaaaaaggc atttccagaa aactctatgg gagttataat gagcataaaa
    34081 agtaatcctc aagatagaga agatgcactt aaggttatag agggatgtga tatagaatac
    34141 cttgatttag accttataga accacaaagt gctatattag acatggtagt aggaaattta
    34201 aaagataaac atttatatag agaagaatat ttaaagatga cagatgcaaa cttaagagca
    34261 agagtaagaa tgagtacaat ttatacaatt gctaacaatc taggatatct ggttgttggt
    34321 acagacaatg ctgcagaaat acacacagga tattttacta aatttggtga tggtggagtt
    34381 gatattttac ctattgcaaa tttaactaaa ggagaggtgt atgaatgggc aaaagagctt
    34441 ggtgttcatg aagatttaat aaacaaagct ccttcagcag gtctttggga aggtcaaaca
    34501 gatgaagatg aaatgggaac tacttacaat atgatagatg ctgtattaga aggtagattg
    34561 gatgaagttc ctaaaagaga ccaagagata attgaaagac ttcatagatt aagtgaacac
    34621 aagagaaaaa cgcctgcaca acctcctaag ttctaattta ctatttataa gatttgtgat
    34681 aaaatttaca agtgatgaaa attagaataa ttaattgtac gtattaagga gtggaaatat
    34741 gggacgtata ggtaatataa ttaatagaaa aggtaaacaa gatgctcaaa gagcaaagat
    34801 atttactaaa catgctagag caatagcagt tgcagctaag gaaggtggag cagacccaga
    34861 gtacaatgca gcgttaaaaa cagctataga aaaagctaag gcagataata tgccaaatga
    34921 taatatagat agagctatcg ctaaaggtgc tggagcaggt gctggagaag actatgaaac
    34981 tatagtttat gaaggatatg gacctggtgg agttgctgtt atagtagaaa ctttaactga
    35041 caacaaaaac agaactgctg gtaacgtaag atactatttt gacaaaaacg gaggaaactt
    35101 aggaactagt ggatgtgtgt ctttcatgtt tgataaaaaa ggacaaatat tagttggttt
    35161 aggagatgga gtttcagaag aagaattgat ggatgttgca ttagaagcag gagctgaaga
    35221 tttcataaca gaagaagatg gttatgaaat aataactaca ccagaagatt tttctagtgt
    35281 tcgtgatgag ttaaaagcta aaggatatga gtttatatct gctgatgtga agatgatacc
    35341 tcaaactaca actgtattga ctgaagaatc tcatttaaaa atgatgaata aattagttga
    35401 tatgcttgaa gaagatgatg atgttcaaga tatatatcat aactgggaag tagaataaat
    35461 agtataagca tattgaaatt tataagtatt gaatcaattt taacatgtga taaattttat
    35521 agaataagaa acactaaaat agacatacgc acctctaacc tatatttgca taataatatg
    35581 taagcatagg taaggggtgt ttttatattt ataggttaag aaatttaaag taaattttga
    35641 gctggactcg attgtctata aataattgat gggtatataa cttgttgctg aataccattt
    35701 atacgcccag ttactgtatc aataatcatc ctaagactac gttctgctgt ctcttccatt
    35761 tttaaatcta taactgtcaa aggaggaata cttcttgcca ttagtcgtga aggtccatta
    35821 cttgctgcaa atatttgaat atcctctcca attacaattc ccgattcatg aaatgcattc
    35881 atcataccaa gagctaccac atcatgatta caaaaaatta ctttttttag tgaatttttt
    35941 gcaagcattt tttgagcaat atcatatcca ctatctattt tattagaaca ataatatata
    36001 gaatctgata gatgtattcc atgttcttta catgttttta aaaaagattt accccttaaa
    36061 gtcatacatg taaaggaagt tgggttgaat acaacagcaa tatcattttt accattttga
    36121 atagcatgta aagctgctat acgaccagtt tcctcattat caatagatac acttgaaaaa
    36181 ccaggtattt cacgatttac aacaactaca ggaattttag gtttattttt tactagatat
    36241 tctatatcat cagcattgat acctgttaaa acaattccat cataataatt atttgcaaaa
    36301 aagtattcat catcacatag atgaccaact ttgtaaggtc gtatgatgat atttatgttt
    36361 aaatgttcta aagaaattat gtggtttatt gcttgaagta tattggttga tgccaactca
    36421 aatccctcat cctgccaata aaaagctatt ttaatattat tatgtttaat aggagtagag
    36481 cgtttatatc caagcttatc agctgaattt aatataagct cttgtgtatg tagcggaatc
    36541 ctgcgttcat ttcctttacc acttaagacg tacgagacag ttgctgaaga aagaccacag
    36601 tcttttgcaa tatctttgat tgttgccatg ataaaatcct ccttaaatag ttgcttaaat
    36661 ttgcttatac caataatctt aacatatatt caactaaaga acaatattct acaaggtgca
    36721 tattttacag gattagaata aataaattaa aaaaaacttc ttacttatgt tgacataatt
    36781 tagtttatag gattataata tgcttaaata agcttatgat taacttgaat taagcaaagt
    36841 taagcaggaa catgcaatgc tagcttgcaa tgtgactgca aagggggaaa aggtatgagt
    36901 aaggtaaata tatttgaagt tggtcctaga gatggatttc aaaacctgaa agagttttta
    36961 gaaactgata aaaagcttaa gattattgat ggtcttattg cttctgggat taaacaactt
    37021 gaaattagtt catttgtaag cccaaaagca atacctcaga tgaaagatgc aaaagaagta
    37081 gcaacttatt gtgtagaaaa atatcctgat gtgaaacttt atgcattagt tccaaattta
    37141 tatggtgcta aagttgcatg ggagtgtggt ataagagata tatcctatgt tatatctttg
    37201 agtgaaactc ataataaaaa aaatataaat cgtacccatg aagagtctat tgatgagctt
    37261 aaaaaaataa tgaatactta tccagacatg aatatatgta ttggaatggc tactgtattt
    37321 ggatgtcctt ttgaaggttt accaaaaatt gaagatgcca taagatttgc taaacgcctt
    37381 tgggaaaacg gtatcaggga gataaatcta gcagatacaa tagggattgc tgaccctaag
    37441 caagtaagag aaactattag tgcattagtt gaagctttac cagaatgcag atggcaagta
    37501 catattcatg atactagaaa tatgggaatg gttaatactc ttgtggctat tgaatctgga
    37561 attactactg ttcagtctac acttggagga ttaggaggat gcccatttgc tcctggagca
    37621 tctggcaata ctgcaactga agatttagta tatatgcttg aacgtatggg gtatgataca
    37681 ggagttgatt ttgttaaact tcttgaatta tcaaagtatc agaagagttt tgtaaatgga
    37741 gtatatagtg gtcatcatgt aaatataaag tctgatagtt gtaattgtta gttctattga
    37801 ttaatcgatt aaaaaaatat gttgattaaa aaaatatgct gattggtaaa atagatttat
    37861 tgataaaaaa tataaattat aaaatacatt atctaataaa acaaataaaa aaattgaggg
    37921 ggattagtat ggatattata gctttacttt tatttttagg agttattatt attgcatttg
    37981 tacgtaagaa taatgcaggt attttggctc tagctattgg tgtcatagct gttagaattt
    38041 ttggaatgga tgataaagaa ctttatggag ctataagcaa ctcaatgttt actacacttg
    38101 ttggtataac actactcttt gcaatagtga actcaacagg agcattaaag cttgttgcag
    38161 aaaaaattgt agctgcttct ggtaaacgtg tttggcttat acctatagca gtttttatag
    38221 caggatttgt tgttgctggt gttggacctg gggcaattcc agcacttgca attatcccag
    38281 caatggctgt tcctgtagcc ataacggttg gttataatcc tgtaatgctt gcacttatag
    38341 gtgaatgtgg attaatggca ggtcgtatga ctcctattac tccagaaggt caaattataa
    38401 caaccgttgc agaaggggtt ggaattgaaa atgttatgcc tactatctta gcctgtcaaa
    38461 ctctttctac aattgtattt tcaatagtat tgtttataat tttcaaagga tataaactta
    38521 aaaaacctat aaatgttata gatagtaagc aaatagaaaa attcaatata aagcagataa
    38581 tttctctaat aagcatagta gttatgattg cttttatcat aatttttgat atgaatgttg
    38641 ggcttgcagc ttttgcagtt tcagctgtac ttattttttt caatattgct gatgaaggag
    38701 aatgtatcaa atcaattcca tggagcacga tagtaatggt tcttggagta ggtgcaatga
    38761 tgacaatcgt agatgcagca ggaggtattg acttgatgag taatgcatta tcatcactga
    38821 tgaatagtaa aacagctact ccaattatga gtatttcagc aggacttatg tctatggtta
    38881 gttctgcttt agcagtagtt tatccaacta tgatgcctat gtgtgttgat atagctaagg
    38941 cagttggagg agtagatcca ctagcactta ttgctgcagt tggtgttgga ggttctcttg
    39001 ctggtgtaag cccactttct actggtgggg cattgatttt agcagctatg ggttcaagta
    39061 agaaagactt taataaggaa gaacaaaata aggtttttgt acagcttttc attatgagtg
    39121 cagttggctt attggttatt gcagtagtat caatattatc atttaatgca ataacacatt
    39181 taatgaacta attagaataa ctgaaaataa atcattaaat ttaactttta atattaatca
    39241 atataatata atttggaggg gaagttatga aaaaaggagc attaagcaat ataaaggtac
    39301 ttgaccttac aagagttctt gcaggtccat attgtacaat gatgttggcg gattttggag
    39361 ctgatgttat aaaaatagag atgccaagaa aaggtgatga tacaagaggt tttggacctt
    39421 ttgtaaatgg agagagttta tactatgcaa atataaatag aaataaaaaa ggtatcactc
    39481 taaaccttaa atcagaagaa ggaaaaaata tatttttaga aatggtaaaa catgctgatg
    39541 ttgtagtaga aaattacaga ccaggtgtaa tggaaaagtt aggtctgggt tatgatgagt
    39601 tggctaaagt taacaatcaa ataatttatg gagctgtatc aggatttgga tgttatggac
    39661 catattcaga acgtccaggt tatgatatta ttgcacaggc aatgggtggt cttatgagta
    39721 taacagggca agaaggagga gaaccaacga gagttggtaa tgctatgggt gatgtactag
    39781 gtggaatgaa tttaactatt ggtatactca cagcactcaa cgctagaaaa ataataggaa
    39841 aaggacaaag agtagatgtt tccctcgttg attctgttgt atcgtctctc gaaactggta
    39901 tacagagata ctttgattca ggacaacctg aacttatggg taatagatat gcttcagcct
    39961 atccttatga ctcttttaag gcaagtgatg gaagttttgt aataggctgt ggtaatgaca
    40021 aactttttga aaagttatgt aaaaaagttc tttttagaga agacctttta atagatgaga
    40081 gatttaaaac aaatcttaat cgttgtgaac attacaaggc tcttaaacca gaaatagaaa
    40141 aatggtctgt taagcataca ataaaagaag cagttgactt aatatcagag gctggagttc
    40201 ctgctgctcc tatctataat cttgattgtg ttacaacaga tgaacatata gcaaaagcaa
    40261 gagaaatgat tgtagatatt agtcatccta ttataggaaa tatgaaagtc aatggaaatc
    40321 cagttaagtt gatgacaaca aaagctaaaa ttgatatgcc agcaccatct ctaggggaaa
    40381 gtaacgagga gatttataat ggttggcttg gttttgatag tgataaaata aaagatttaa
    40441 aagaaaatgg agttatttga ttaaaaaata gctaaacaca cttgtgactt tagtcatggg
    40501 aggttcagtt agaataatat caaaaaatat taaatcaata ctgtaaataa aatatagaat
    40561 tgtcagaagc atttaagatg ggaaaagcta cttagacatt gagtctttgt ggcttttcct
    40621 attttcatta agaattttat attatcagaa tagatatgta ctaaactaaa gtattaacaa
    40681 tttaaattaa tagaaaataa actacctaat tatgttgaat aaagactact ttagtgaaaa
    40741 caaacttata gagatgctat ttaaattatt ttaatgggaa tatcaaaaat gagactgcat
    40801 tctcaaaaat gagactgcgt tttttaagta ttttctcgaa aatgagacaa aaatattatt
    40861 ttatgggttt tattgaagaa aaattcttct gtgaattaca atttaatcaa ttttaaagtt
    40921 gaaaattgtt aaaaatttag tgaaatttat gtatatttcg tttattttat taaaaataac
    40981 aattttttac taaaaataaa gaacttcaat ggtgattggc atagtacttg ctattttata
    41041 ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat ggaatctgta
    41101 atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt tattactata
    41161 aatagaccta aagctttaaa tgctcttaat agtgacactc ttagggaact atcacaagtt
    41221 attgatgtag tatcagaaaa tgaggcaata ttaggagtta ttattactgg agaaggtaaa
    41281 gtttttgtag ccggtgctga tatacgccag atgcaaaact ataaaagtga ggaaggcaga
    41341 aaatatgcag gttatgctca gggaattttt gataaaattg aagctcttga gaaaacagta
    41401 atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat gagttgtgat
    41461 attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt aggagttatt
    41521 ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat agctaaagag
    41581 ttaattttta cggggagaca agtaaatgca gaagaagcta aatctattgg gcttattaat
    41641 aaagtggttc caagtgattt acttttagaa gagtctatga agatgatgaa ccaaatagtt
    41701 gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaagg gatagatatg
    41761 gatttaaaga atgctcttga actagagaaa gatattgcag gtttaacttt tgctacaaga
    41821 gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt tgaaaataag
    41881 tagctattat aactaaaatt ggaacaatgt agaatatatt caaaatctaa atttcgtgaa
    41941 aaaatacata tagtttaatt ttaaaaggag ttaatttatg aatacgggag taattataag
    42001 tttagtaggg attatcatag ctctatctct tctagttgta atggttatga agggaataaa
    42061 tatttttatt atagcaattg tatgttcatc attagttgct ataacaggaa gcctaaatgt
    42121 gtatgatgca cttaaagtgg attatatgac agggtttgtt ggatttcttc aggcaaattt
    42181 ctttattttc ctaacaggta cattaatggg aaaaataatg gagattactg gtggagcaaa
    42241 atctattgct aagatgattg taagatggat tgggaaggat aaagcactac tttctattcc
    42301 aatagcttgt ggaatattag cctatggtgg agtaagtgtt ttcgttgtaa gttttgcagt
    42361 gtttccgata gctttagaaa tttttaaaga agctgatttg ccacgaagat ttataccagc
    42421 agctttgact tttggatgct caacttttgc tatggttgca ccaggggcac cacaaattca
    42481 gaatattgtt ccagcatcaa cattagggac agatattatg gctggagcag taaatggttt
    42541 tatatcatgt gcagtaatgt ttatagttgg aagtacaatt ttgtacaaaa tggtttcaaa
    42601 agaaaaagcc aatggtggtc attttatagc acatgaatca gatatatttg atgaagcaga
    42661 agcaactaat tctaagagag aaaatggtcc caatggtgta attgccttaa ttccattgat
    42721 agtgtcaatt ttaataatta atgtaaaagt taatggaaaa gcgatagtac caattgaggt
    42781 gggagtattt attggagctt tattagtata tttattgtta aataaatatc aagataatgc
    42841 taaaatagtt ggacatgttg gtgatgcatg taaaactaca gtagtagcaa tatgtaatac
    42901 ttgtgctgta gttgcatttg gttcagtagt aaaatcagca gttggttttg acttcatagt
    42961 taatgctatg actaatattc ctggtcctcc tattgcaggg gctgcagttg gaactacagt
    43021 tattgcaggt atttgtgggt cagcttcagg tggactagga attgcagctc cacttcttgg
    43081 accagtattt ttagccaagg gtgtttccgc tgcagcatta actaggacta tggcaatttc
    43141 atcagcagca ttagattcac ttccacacaa tggatatatt gttactgtaa caaatggtct
    43201 ttgtaaagaa acacataagg atgcctatat gccaatattc tgggtaactg ttttaacccc
    43261 atttattgga accatagtag cagtcctact gtttacaatg ttcccgggat tgccataaga
    43321 gaaaagataa aaggtataaa ttatattttg atgttataaa atcaattttc aaaatatata
    43381 caataaattt catgttttaa tactgtaaat atgtatttaa aataacaaat tgaccatata
    43441 aggaggaatt gaaagtgtta aataaaccat tagagggagt taaggttatt gacctaacgt
    43501 attttgtagc aggtccaggg acttctaaaa tattagctga ctggggtgct gacgttatta
    43561 aggtagagcc aagctttgga gacccaggaa gaaagacagg ggcaacaatg acaatgccaa
    43621 tagatgatta taataatcct ttttacagca catataattc aaataaaaga ggtttgtcta
    43681 taaatcttaa gagtgaaact ggaattgaaa ttatggataa gcttttaagc gaagcaaacg
    43741 tatttgtttc aagttataga acaggagcat taaaaagact aggtcttgat tatgaatcat
    43801 taagtaaaaa gcatccacac cttatctggg gtcagataaa tggttttggc gattttggac
    43861 ctgctaagga taatgcagga tttgatacag ttgccttttg ggcaagaagt ggggctatgc
    43921 ttgatatagc agaaaaagat acaagtccaa taaatccacc tatagccttt ggagatgcaa
    43981 caacttcttg tagtttatct ggaggaatat gtgcagcttt gtaccagcaa gctaagactg
    44041 gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc aatatggaat gcatcatctt
    44101 tattagcatc tacacagttt agtgatgagt atccaaaaac tagaaaaaat gctatttcac
    44161 cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat attcttaagt attttagagc
    44221 atgaaagata ttttgaggca ctttgtagat tatttggaag agaagactta ataaaagaag
    44281 agaaatttgc atcttcagta gaaagtaaga aaaatgccac agaacttatt agtattttgg
    44341 atggtgaatt tgcaaagttt actcaaaatg agatggttga aaaacttact gctgctgata
    44401 tagctcatga aaaaattcaa catgttaatg atgtagtgac tgatgaacag gctattgcta
    44461 ataactatat atatgaacat acaagtaaaa atggaaataa gacaatgctt gcatctactc
    44521 cagttaaatt tggaaatatt gaagttaaca tgacttgtga tgctccactt ataggagaac
    44581 attcagatga gatattgaga gaacttggat acagtgattc tgatatagag gctctaattg
    44641 aatctggaat tgtaacaatg aataaagaag catgtgaaat atgttaataa acatgtgaag
    44701 tatgttaata aataggaggg aattttatgg attttagatt aacagaagca caattaatgc
    44761 ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc accaatagct gctgaaacag
    44821 ataaaacagg tatttttcca agggagcttt ttagcaaaat ggctaaaata ggatttaatg
    44881 gaattggaac acctgtagag tatagtggtt ctggtggggc agatatagaa aaggtaattg
    44941 ttgttactga gattgccaaa aaatgtgcag catctgcagc aatattatca atacacacaa
    45001 tatatgctca agctattttg aaatttggta ctgaggaaca aaagaaaaaa taccttccaa
    45061 tgatggctga aggtgggtgt gtaggtgctt ttgccttaac cgaacctaat gctggttcag
    45121 atgctgcaag agctgcaaca actgcaatta ttgatgaaga aactgatgaa tatgttttaa
    45181 atggaactaa gtgttttatt tctggtggag gtcaagcaga atcattaata atatttgcat
    45241 tgacagaccc atcaaaggga ataaagggaa tgtctgctat cattgtcgac aaaggtactc
    45301 caggtttttc aataggaaaa attgaagaaa aaatgggtat tcatggttca gaaactgcag
    45361 agttaatatt tgataactgt agagttccaa agtctaactt attaggaaaa gaaggcaaag
    45421 gatttaatat agctatgact tgtttagatg gagcaagaat tggagttgga gctcaagctg
    45481 taggaattgc tgaaggggct ttagaagaaa gtatcaaata ttctaaagaa agagtccagt
    45541 ttggcaagcc aatttcagct ttgcaaggta ttcaatggta tatagcagat atggcgacaa
    45601 tggtggaatc agcaaaatta ttagtatatt atgctgcaga tttgaaagct agaggagaaa
    45661 aacacactaa agaagctgct atggcaaaat acaatgcgtc acgtactgca agagaggtta
    45721 caaatttagc ccttcaaatc catggtggat atggatatat gaaggattat ccattagaga
    45781 gaatgtacag agatgccaaa ataacagaaa tttacgaagg tacttcagaa atacacaaag
    45841 ttgttatttc aagagcagta ctaggatagg aggaaaagaa tgaatatagt tgtttgctta
    45901 aagcaagttc cagatactaa tgaagttaaa ataaataaag agacaggtac tctaataaga
    45961 gatggtgtac caagtataat caatccagat gatagaaatg ctttagaaga agctctaaaa
    46021 atgaaagatg aactaggagc agtgattaag gttataagca tgggacctcc tcaagctaaa
    46081 agtgctttaa aagaagcttt ggcaatggga gctgatgaag cttatttaat ctcagataga
    46141 gcttttggtg gttcagatac ttgggctaca tctacaatta ttgctgctgc tatagagaag
    46201 gttggaaagt atgatgtaat attctgcgga agacaagcta tagatggaga tacagctcag
    46261 gttggaccag aggttgcaga atttttaggg ataccacaag ttacttatgc gaaagaagta
    46321 aaggttcaag atgacaaatt actagtaact cgttatacag agacagggga ttatttaata
    46381 gaagcaaaga tgccagtttt attaactgct ataaaagagt taaacaatcc aagatatcca
    46441 agtgtcaaag gaatcttaga agcttataat aatggagatg caaagattac tgtattaaca
    46501 cttgcagatt tagatgttga tacaactcaa attggattaa agggttcacc tacaaacgtt
    46561 tataagtctt ttgtaccagt taaagacaag cataatgaaa taatagaagg tataaataaa
    46621 aaagaaaaag ctgagaaact aatagaaata ctattcgatt tgaaactagt ataggagtgt
    46681 taagatgatg agagcaaaag ttaatcaagg tataaattta aatgattata atggtgtttg
    46741 ggtaatagga gagcaaaggg aaggtaaaat taatcctgtt acgatagaac ttattggtga
    46801 aggaagaaag cttgctgacc aattaggaaa agagcttgca gttgtgatag ctggatatga
    46861 agtagagaaa gaagtgaaag aattacttca ctatagtgtt gataaagtat attacatcaa
    46921 tgaccctctt cttaaagatt tcactactga tggatatgca atttcaattg caaatttaat
    46981 cgaaagaaaa aagccagagg ttgtattggt tggtgctact tctataggaa gagatatagc
    47041 gcctagaatt gcaggtaagg ttggaacagg tctaactgct gactgtacaa aacttgaaat
    47101 agattcaaca gataataagt tattacaaac aagaccagca tttggtggaa atttaatggc
    47161 cactattgtt tgtccaaaaa atagaccaca gatgtcaaca gttcgtccag gagttatggc
    47221 aaaagctgta agaaacgaat cagagactgg aattttagaa gttgttactc cagaacttac
    47281 tgaaaaaatg attagaacta gattagtaga gatacttcca caagaaaaga aatctgtaaa
    47341 tctaactgat gctagaataa tagtatcagg gggaagagga ttaaaaagag cagaaggatt
    47401 tgagcttata aaagaattgg ctgataaatt gggggcagag attggtgctt caagagcagc
    47461 agtagattca gggtggatag aacattctca tcaagtggga caaacaggaa ctactgttag
    47521 accagaacta tacattgcat gtggaatatc tggagcaata caacatttag caggtatgag
    47581 tgattctaaa tatatagttg ctataaataa agatgcaaaa gcccctattt ttagtatatg
    47641 tgactatgga atagtaggag atttatatga aattatacct gaaatgatag aatcattaaa
    47701 taggtaactt ttagaaaaca ttgaatatgt agaatgataa gtcttataat attgtattgt
    47761 aagacttatt atttttgctg aaggttatag tggggagatg aaacaatgaa agagcagtgg
    47821 tataaggata tatttgcaag agtattatct atgactgatg atggttttat tgttgtaaat
    47881 acaagtggag taataataga tattaatgat aaatattgtg actttttagg aaaagaaaga
    47941 aaagatataa taggacaaaa tatacaaagt attataccaa atacaaaaat gctagatgtt
    48001 atgaaaaata agtattgtga agaaggagct atacatcatt attcaggtgg aaatacaaaa
    48061 gaaaaaagtg taatagtaag taggtcttat gtggaaaatg acaatggaga agtagttgca
    48121 ggagtagctc aagttaaatt tagattgcaa tcatttgatg tagctaaaaa gctaatgtca
    48181 gaatatatgg agctccaata ttataaagag cagtttaagg ataactgtgg ttttgacaag
    48241 ctcataggtg aaaatagaga cttcatagaa cttaagaaaa caggtgtgaa agcttctaaa
    48301 acaaactttc cagtactttt aacgggtgaa acaggtacag gaaaagaagt ctttgctaga
    48361 gcaattcaca ataatagcag caggtcagat aaacctatgg ttagtataaa ctgcgctgca
    48421 ataccagaag aattattaga gtcagagcta tttggttatg acgaaggagc ttttacagga
    48481 gcaaaaaagg gtggtaaaaa aggtaagttt ttagttgcaa ataatggtac aatcttttta
    48541 gacgagatag gtgacatgcc tttgaccatg caagcaaaac ttttaagagt tcttcaggaa
    48601 agtgagatag aaccagtggg aggtcttaaa actataaaga tagatgtaag agtaatctct
    48661 gccacaagaa agaatttaag taaaatggtt gaagagggat tatttagaga agacctatac
    48721 tatagattaa atgtaataaa cattcatatg atggaattga aggacaggca agatgatatt
    48781 ttattgctag caaattatat attaaacaaa ttaaatgttg aatacaagga attgaaggtt
    48841 ttaagtgata aggttaaaaa ttgttttatt aactatactt ggccgggaaa tataagagaa
    48901 ctacaaaatg taataaaaag tgcctatgct gtaagtgatg atatggtcat tatgatgtgt
    48961 gaccttccat ctaagatgga taatatcagc agagtagctc aatgtaatgt ggatagcaat
    49021 tgttcaattc atgaaatggt tgagaattat gaaaagagct taattattga tgtgttaagg
    49081 aagtataatt ggaaatgttc taaggcagca gaggttatgg gaatacacaa gagtctttta
    49141 tataagaaaa taaaaaagta tgaaattgaa ttaaataatt aagataaatt gaattaaggc
    49201 aatgctaaaa aaacgccctt attcaaagcg aactgtataa actgttttat gcagttaaaa
    49261 cgtgtaaaag tagatgtgaa gtactaatgt caatcattag tactttttta ttatatttat
    49321 aattactatc tatatttcag tatcttttga tatctaagat tattatgttt aatttaggat
    49381 ataacaaaac cctcttagtt atggacagtt ttttatattt taactgtcta ctaagagggg
    49441 atagtttagt ttaaatttag agtaaatttt atctagcatt tataaaaaat attttgaata
    49501 gcttttttgt tatttaatca taattttcta cttctttatc aagttctttg ccacaagaat
    49561 cacacttata tatagcattg tatccgtaat catattcatc aactattttc ttttgtctaa
    49621 atacaccacc acactcacat aggaaaccaa tttttataag tgtaacgata aattgtgatt
    49681 ttataaattt tatatcatta catttatcgt tagtgtaaaa tgtataaata taagagttat
    49741 cgcactcatc tcttaattca aatctttcaa tatatgtgtt attttttaat tcctcaaatt
    49801 cttctttagt taaatatgat ttttgttgca attcatgtaa acgcttaatt tggtccatta
    49861 tttatatcct cccaataatt ttagtatttt aagcttagat aaggaatcta agttttatta
    49921 aatttatccc aaggaattat ttaaatcata tttagtttta ttaattacaa catatataaa
    49981 ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt gaaaacctaa actactaacc
    50041 ccttactaac ccgaaaacta aaaaaagaaa atatatccaa atataagtga tatataaata
    50101 aagatgtttc atataaattt aactttaaat attctgacaa taatatatat aaatatatta
    50161 tagttaaaat ataccacata ttttgggtat tgtatactgg aattagagta attaatttat
    50221 tttttaacat acaaatgatt ttatgaaaaa aaacaagact caaaaatttt tatattcttt
    50281 gatagaataa aattgagagt aaatttattg aattttataa aatttataag gggtgagtct
    50341 aagatatgca tgaagataaa aatgatatat ttgaatcaat agatactgag gattttgata
    50401 ataaaacaga agaagaatgt ttagagctag taagaaaaaa tggattgaat ttaatataca
    50461 taaaaaatca aacagaagag atatgtctag aggctgttag acaaaattgt aatgcaataa
    50521 aatgtgttca gaatcagaca gaaaaaatat gtatagaagc agttaaacaa gattggagaa
    50581 tgttagaatt tgtggaagag ccaacagaag aaatttgcat gttggctatt aaccaagatg
    50641 gaacagcctt aaaatatata gaaaatcaga cagaggagtt atgcttaaga gccattgaaa
    50701 aaaatggtgc ggctttggag tatgtaaaag aacaaacaga agaaatatgt atagaagcag
    50761 ttagaaacag cgaatttgga ttggcaagag tgaaaaatca aacagaaaaa atatgtatgg
    50821 aagcagttaa gcattgtagc tataatctaa aatgggtaga aaatcaaaca gaagaaatat
    50881 gtatggaagc agtgagacaa aatgggcttg atttgaaatt tgtaaaaaat caaacagaaa
    50941 caatctgttt aagagcagtg agacaaaatg gaatggcatt agaatttgta aaggaacaga
    51001 ctgttggtat atgcttaaag gcagtaagac aatatggaat ggcattaaaa tttgtaaaag
    51061 aacaaacaga agaaatttgt acagaagcta taaaacaaga taaaagagca cttagttttg
    51121 taaaaggaga taaggaaaaa tacaaggctc tatatgataa taatgaacca tttgcaaaga
    51181 gatatgttag aaatgtaata gaaaaagaaa acagagcatt gattaaaaaa ggagaagaaa
    51241 atgcaaaaat caaaattgca ggaaaacttt atgatagagg catgtctttt gaagatatat
    51301 cagatatagt tgagattgaa ataagcaagt taaaagtgag tttaggtgtt ttttaaagta
    51361 ataaatgtag gtgtttttta tgggaaatag ttaataaaaa aataatcgac tagctctttg
    51421 taggacagca aaattagtga tataatttga tgtataataa attattttta ttagataagg
    51481 gggaattaaa gttgaagatt acagtattat accaaacaag aagtggaaac acagaaaggg
    51541 tcgctaagct gatagaagat ggagctaaaa aggtggatgg agtagatgta aagcttatga
    51601 gacttgataa tattgattta gattatttaa atgagagtaa agcagtaata tttggaacac
    51661 ctacttattt agcaaataca acttgggaag taaaaaaatg gtttgatgaa gattcaaaaa
    51721 aagttaattt agctggaaaa ttaggagctg tttttgctac atgtgattat atatgtggag
    51781 gtccagatgt tgcgatactt actatagtag gacatttgat ggttaaagga atgcttgtgt
    51841 attctggagg tggttcttta ggaaagccat ttatacattt aggacatgta cattcaattg
    51901 aaggtccaga gttacaagat gagaaggcta taatatttgg tgaaagaatt gctactaagg
    51961 caaaagaact atttgcttaa tttctatatg cataataatg attagagtaa atttagggtt
    52021 ttaagggaga tttgaagtaa aagttataaa ataaaaaatt ttccataaaa ttactcaaga
    52081 tgaattataa tataattata aataaattat attattaaaa tatttaaaat caaacgtcaa
    52141 tagcgaggga agtttatggt atggcatata gaatatgaga ttttttctgc aattatagtt
    52201 atttttttaa tggtatactt ttttagaagt aaatttatac ctactttaca aaacaaaata
    52261 tattgtgcac ttttaatatt ctcattttta tttataattt caaatatttt aggttcattt
    52321 tgtctaaata atatagacaa aattccaatt tttattactt ttttattgaa tcagatatac
    52381 ttattgttat tgccaatacc agcagcactt gtatcatttt atgttatggc tattatttat
    52441 caagatataa ggtatatgaa aaaaagatta ttatttttat ctataccact tataatttgt
    52501 ataatcttat ctattacaaa tccttttacg catattctat ttggattgtc aaaagaaaca
    52561 ggttatatac gaggttgtgg atatgttgca acttttgcat cttttttctt ttatgcaata
    52621 tattcagcat ttttatcttt taaatataga aaagcaatgc atgaatcaaa aatatgggct
    52681 attagaggtt ttcttatagt ttcaattgtg gcaattatct tacaggcaat ttttttacag
    52741 tatcttctta caggaactgc atgtgcatgt agtttacttt tgacatatct ttctatgcag
    52801 aatcgaggtc ttattattga tgaccttact ggtgtactca atagacaatc ttttattcaa
    52861 gagctagatt tgaatatcaa tactgaacaa tcaggattta ttattacaat tgcgcttgat
    52921 gattttaagt ttatgaatga aacttttgga acaaaaaatg gagatattgc actaaaagaa
    52981 gttggaaaat atttgattca gatacatgat gtaaaccatg tatatagaat agggggagat
    53041 atattttcta ttgttgtcaa tagtcaatta ggtatagaac cagatgatat tatcagaaag
    53101 atagaagaaa gatttaaaaa gccttggcta gtagaagaga taagttttaa tttagcaact
    53161 agtattgctg ttgtttatta cccagaaaat gcagaaacga cagaagatgt aattgttgct
    53221 atagatttct ctatacatga agcaaaacgt tctgagtcaa ggcgagttgt ttatgcagat
    53281 gcgtctataa gtgaaaagat aaagaagaaa catgttatta aaaattgttt aaaagaagca
    53341 ttaaaaaatg atgggtttga agtgtactac caacctatat tttctaatag caggggcagg
    53401 tttacttcag cagaggcatt acttaggcta gaacacaaag agttaggttt tatacctcct
    53461 gatgaattta ttccaattgc agaacaaaca ggattgatta actcaattgg attagtagtc
    53521 tttgagaagg tatgtagatt tatagctagt gatgagtttg aaaatttaaa attggataat
    53581 atagctgtga atctttctgt agtacaatgt atgcaaaaaa acttagctga tgatttactt
    53641 tatcttatga ataaatataa tgtatcacca actaaattta aacttgaaat tactgagact
    53701 gtggctatgg gctcttttaa tataattaaa gaaacaatgg aaagattaat tgatttaggt
    53761 gttaagtttg cacttgatga ttttggaatt gcttattctg gagttactaa tatgctttct
    53821 ttaccatttt cagtaattaa gttagataag agtttgattt ggtctatgaa tgaagatagt
    53881 aggcataaac ttacagttga gacgattata gctcttataa ataaattgaa tatgaaggct
    53941 gtagcagaag gtgttgaaac cattaagtgt gctgaagatt taatagctat ggagtgtgaa
    54001 tatttgcaag ggtattattt ttctaaacca gttccagaag atgtttttaa aaatttgttg
    54061 aaagaaaata cttttacaag ttaacaccct ttattttttt aattacaact ttgttcattg
    54121 acactgccag cgatatatta taaaattaac acaataataa atattaaatt tattatacat
    54181 attattaata ttaattaatg gagtaaagat ttattatata ttttatttaa ttatcctaaa
    54241 gtgagaaagg aagatgtgac atgaataaaa agaaaatatt agtgccaatt gatgggacag
    54301 aaagaagtat gcattctttg gaatttataa aaggaatatt taaaaaagat gaagttgaag
    54361 tagaaataat gaatgtaaaa gaactagtat ttattgatgg catatccctg gctgaagaga
    54421 taaaaaattc tgaaaattta ggaagaagaa tcttggataa agcagcagag ataatgggag
    54481 agtatgatgt taaagtacat tttacttttg gatatccagg agatgagata attagaaaag
    54541 caaaagaaga taatgtagat tttatagtta tgacaaaatc tactaaaaaa ggtcttacaa
    54601 gaatgatagg ttcagtaaca gcaagtgtag ttaaacaagc aaaatgtata gtgatgattg
    54661 taccggagta gaattagaaa ggtatttgaa atatgaataa aaagatagta gcagtttgtg
    54721 aaagtgaaag aagtttaaat tctcttaaga cagctgcaaa tgatttagga tttgatttag
    54781 tttatgaaat tcaagaaaat gataatataa aaaatgaact ttctatttat gatatagaag
    54841 atgcatccat agtgttattt gttacaagtg attctataga aagcatagag aagattgata
    54901 gatttataga tagagaattt tatgaagttg atccaaagta tataatagag gatgcaaaat
    54961 ctatagtaag tgaaatttta atagacttaa attaatcgtg ggtgtcgcac taagaataga
    55021 gcgacgcctt tttatatatt ttacacaaga aataaagagg ttgtatcaaa atatagataa
    55081 gactcttttt cattgttgaa atgatgaaaa tatatttgat ttcatacaaa aaagagtacc
    55141 tcataactaa aaagttcact ttgagacact ccatttttat atgttagttg catttaaggt
    55201 aaaatataag tttaatcatg ttgaaataac tgaattacat cttctaaatg attgtacatt
    55261 gatgtataac ctaagtaaga gtctttacta tcaaatgcca tcattatctt gttatggtgg
    55321 ctaatcatat ccttgagaga atttctatat ttaaaaccga agtattggtt agaagaaact
    55381 gttttaggta ttaactcata atattttata aaaattggat tatgagaagc taaaattatc
    55441 tctttgtgaa attgcaagtc agcatcataa cattcttcta tcaaaccatg agaaacctta
    55501 tccaattcct ctattgactt taggtaagta tattttattt tttgaagttc tttagatgta
    55561 gcatttaaaa ttgctagttt agtagcttca ctttcgataa gttgtcttgc tcttaaagat
    55621 tctacataaa attcttgtat gttatgattt ttatagattt caccatcatc aaataggtca
    55681 acttttttaa atccttcaac tataggactt gagtaaggca cagctctaag tattccttcc
    55741 ttacagagta tatttatggc atttcttacg ggtattctac ttatgttaaa taggtcagca
    55801 atctttctct cacttggtaa tttactatta attttaaaag ttccatttag aatgtttaat
    55861 ttaatataat caataatttt ttcagtggca tttaagcttt tcataaaaac tcctaaacat
    55921 ataatttttt tatgattgta ttatatcata gttaagagtt aaatctactt ttttataata
    55981 tgtataagac aggatagagg ccaaaatagc tataatagcc aagataaagt atccatattg
    56041 tattgaagta tattccgata ttttaccaac tatcattgga aatacagaca ttcctatagc
    56101 ataaattgct tgaaaaaaac ccattgcagt agatttctga tgttttggta catttttcat
    56161 agactcgcta gtaaggtaag aaaggagtat acctgtagat atacctggta gcacttgtag
    56221 tacaaatatt atattaatat tttttattgt tggaactaaa aagcaatagg ctgaaactaa
    56281 aataaaaact aaaggaatcc aaaattttgg cccatactta gtacaaaatt tagtagaagc
    56341 aaagccagaa gaagcaacag cacacaccat gtaaaatata gaagatagac ctatgagggt
    56401 agaactagaa cctaagtttt taagaatttg agtagtaaat gacattgttg tagacatttg
    56461 aataccctgt tgaattaatg ccataaagga aaaaaataca agtcttttat ccttgcagat
    56521 agataaatat tttttaattg atattttttc aggtattttt tttgaattat gattttttat
    56581 atttaaggct agaattgttc caataactga agacaataca ctcatgatac ataaggctgt
    56641 cattcctaat ttttcataaa ataaagtact cataacaaat ccaatcaaca ttcctagatt
    56701 gtttaccaat attatcgtac ttgtagctct ttgttgatgt tctgtagaaa aaaagttggt
    56761 atagtgaacc atgtaagaaa tccaagttga tgatgatatt ccagataaaa tgttagctat
    56821 aagaaaacca ttgcctccag gagagaagac tcttataaga gatgctaaac cagcaaagaa
    56881 aattccagac agtataaata ttttatgttt atttatggaa tccgcaaata ctccaacagg
    56941 aagccttaat aaacattgtg agattccata tgcacctatt attataccaa tcatatttgt
    57001 agatacatta catgcactta aatatggatt ttggaaaggt atatatacat attgtgaaaa
    57061 ccagaaaaat gtaacaatag aaagtaaaag taatttttga ttagatattt ttttcatatt
    57121 atcctccgta tggtattttt ataatgcaaa taatatacca tttggatttt ttaatcaagt
    57181 actttatcta ttaaaaaaaa tttaaatata taatcattat ttacaaaaaa attatacttt
    57241 atagttgaat taagaaaacg cttatgatac tataaagtta acaaatttga atatataaca
    57301 gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat ttttatagat tatctataga
    57361 gtactatggg tataatctat ttatttggtt cgattttttt gtttaattgg ccataaaaca
    57421 aaaagcctat agttgaaaat ggaggatttt ttatggatta taaaaatata gctcaagaaa
    57481 ttctactaaa tgtaggagga aaagagaatg taaatgaagt gactcattgt atgacaagac
    57541 ttagatttaa agtcaaaagc gcttctaaag tgaataagga taaattatct aaaactgaag
    57601 gagtaataac tgttgttgag agtatgggtc aaatacaagt tgtaattgga aataaagtaa
    57661 aaaaagtata tgatgaggtc ataaaaattg tacctcaatc aaataacgta ggtaaaaaga
    57721 atgaaagtca agaaaagcaa ggtataatta attctatatt atcagcagta gctggtatat
    57781 ttacacctac aatacctgct atagcagggg ttggtatgat taaaggtatt ttatcagttc
    57841 ttgctatgta ttatatgaat aaaaatggag tagatataaa agaaacgcaa agctatatta
    57901 tcttaaatgc tatggctgat tctatattct attttatgcc tattatacta ggatatactg
    57961 cggctaaagt atttaatgca aataaaataa tatctatggt attaggagct acactttgtt
    58021 atccaacttt tactgctttg atggcaggtg aagaatctgt tagattttta ggattggcag
    58081 taacaaaagc tacgtataca tcatctgtta ttccaataat tattgctata tgggctcttt
    58141 catacgttga aaaagtatta gaaaagtata tacctgaggt aattaaaata ataatggttc
    58201 caactttatc attggttgta atgttacctg caacattatt tttatttgga ccaatcggaa
    58261 tttatatagg aaatgtcata aacttttctt ataagtatat ttatgaatta agcccagcat
    58321 tgtgtggagc atttgtaggt gggttatggt gtgtattggt tatatttggt gctcatagag
    58381 cattattacc aataggtata agtgatgttg ctcaaacagg aagacagaat ttattggcgt
    58441 ttgcaggatc tgcaaacttt tcacaagcag gggctgctct aggagtattc tttaaaacta
    58501 aaaatcaagg actaaaaaca atttctatgt ctgcaactat aacagcttta tttggtataa
    58561 ctgaacctgc aatatacggt gccaatctta gattaaaaaa acctatggta tgtgccgtaa
    58621 tatgtggtgc atttggaggt gcgataatgg gaatgggtgg agcttatggt aatgcatttg
    58681 caaaccaagg tgttttaact atacctgtat atgctgaagc aggtgcatta ggtttcttaa
    58741 gttatctagg tggttgtgct atagcatttt ttggttcagc tatttcaact tatctagttg
    58801 gatttgaaga tttggaagag tcttctaata aggaaaattc atctataaaa gtagaaacga
    58861 aggatggtgt tattgatata acttcacctg tagagggcga atgtatagaa ttgtcagaag
    58921 taaaggatga tgtatttgct tcaaaagcta tgggagaagg tatagctgtg ttacctacaa
    58981 aaggagtaat aacagcccct actgattgtg aagtagcttc tttatttcca acattacatg
    59041 caataggatt aaaattagat aatggagcag aaatgttaat acatgttgga atcaatacag
    59101 tagagttaaa tggtaaatac tttacaaaac atgttaatca aggagattta gtgaaaaaag
    59161 gtgataaact tatatcgttt gatatagata aaataaaaaa ggctggttat gatgtaacta
    59221 cgccagttat agtaaacaat acatttgatt aaaaaatagc tact
//
LOCUS       DAMJYC010000013        59020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJYC010000013 DAMJYC010000000
VERSION     DAMJYC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.013"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(43..234)
                     /locus_tag="PC472_000887"
                     /pseudo
     CDS             complement(43..234)
                     /locus_tag="PC472_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            504..1145
                     /locus_tag="PC472_000888"
     CDS             504..1145
                     /locus_tag="PC472_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2726237.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            1171..2715
                     /locus_tag="PC472_000889"
     CDS             1171..2715
                     /locus_tag="PC472_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2726238.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(2928..3554)
                     /locus_tag="PC472_000890"
     CDS             complement(2928..3554)
                     /locus_tag="PC472_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2726239.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(3689..4405)
                     /locus_tag="PC472_000891"
     CDS             complement(3689..4405)
                     /locus_tag="PC472_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2726240.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            4831..6045
                     /locus_tag="PC472_000892"
     CDS             4831..6045
                     /locus_tag="PC472_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2726241.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            6663..7991
                     /locus_tag="PC472_000893"
     CDS             6663..7991
                     /locus_tag="PC472_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726242.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(8119..9309)
                     /locus_tag="PC472_000894"
     CDS             complement(8119..9309)
                     /locus_tag="PC472_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726243.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9415..10794)
                     /locus_tag="PC472_000895"
     CDS             complement(9415..10794)
                     /locus_tag="PC472_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726244.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(10825..12033)
                     /locus_tag="PC472_000896"
     CDS             complement(10825..12033)
                     /locus_tag="PC472_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726245.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(13058..14512)
                     /locus_tag="PC472_000897"
     CDS             complement(13058..14512)
                     /locus_tag="PC472_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2726246.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(14532..16403)
                     /locus_tag="PC472_000898"
     CDS             complement(14532..16403)
                     /locus_tag="PC472_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2726247.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(16393..17253)
                     /locus_tag="PC472_000899"
     CDS             complement(16393..17253)
                     /locus_tag="PC472_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2726248.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            complement(17848..18252)
                     /locus_tag="PC472_000900"
     CDS             complement(17848..18252)
                     /locus_tag="PC472_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2726249.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(18444..19631)
                     /locus_tag="PC472_000901"
     CDS             complement(18444..19631)
                     /locus_tag="PC472_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2726250.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(19663..20088)
                     /locus_tag="PC472_000902"
     CDS             complement(19663..20088)
                     /locus_tag="PC472_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726251.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            complement(20216..20368)
                     /locus_tag="PC472_000903"
     CDS             complement(20216..20368)
                     /locus_tag="PC472_000903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726252.1"
                     /translation="MRRDNNLLMKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFVLV
                     FLFTIT"
     gene            20566..21315
                     /locus_tag="PC472_000904"
     CDS             20566..21315
                     /locus_tag="PC472_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2726253.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(21481..21846)
                     /locus_tag="PC472_000905"
     CDS             complement(21481..21846)
                     /locus_tag="PC472_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2726254.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(21862..22638)
                     /locus_tag="PC472_000906"
     CDS             complement(21862..22638)
                     /locus_tag="PC472_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2726255.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(23049..24476)
                     /gene="ascB"
                     /locus_tag="PC472_000907"
     CDS             complement(23049..24476)
                     /gene="ascB"
                     /locus_tag="PC472_000907"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2726256.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(24537..26465)
                     /locus_tag="PC472_000908"
     CDS             complement(24537..26465)
                     /locus_tag="PC472_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2726257.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(26610..27452)
                     /locus_tag="PC472_000909"
     CDS             complement(26610..27452)
                     /locus_tag="PC472_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2726258.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(27799..29163)
                     /locus_tag="PC472_000910"
     CDS             complement(27799..29163)
                     /locus_tag="PC472_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2726259.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASV
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            complement(29202..29873)
                     /gene="pgmB"
                     /locus_tag="PC472_000911"
     CDS             complement(29202..29873)
                     /gene="pgmB"
                     /locus_tag="PC472_000911"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2726260.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            complement(29875..32265)
                     /locus_tag="PC472_000912"
     CDS             complement(29875..32265)
                     /locus_tag="PC472_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2726261.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(32291..33739)
                     /locus_tag="PC472_000913"
     CDS             complement(32291..33739)
                     /locus_tag="PC472_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2726262.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(34144..35031)
                     /locus_tag="PC472_000914"
     CDS             complement(34144..35031)
                     /locus_tag="PC472_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2726263.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(35131..35580)
                     /locus_tag="PC472_000915"
     CDS             complement(35131..35580)
                     /locus_tag="PC472_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726264.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(35570..37477)
                     /locus_tag="PC472_000916"
     CDS             complement(35570..37477)
                     /locus_tag="PC472_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2726265.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGIYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNVNNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(37745..39640)
                     /locus_tag="PC472_000917"
     CDS             complement(37745..39640)
                     /locus_tag="PC472_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726266.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVVTALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(39676..40554)
                     /locus_tag="PC472_000918"
     CDS             complement(39676..40554)
                     /locus_tag="PC472_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2726267.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(41097..41267)
                     /locus_tag="PC472_000919"
     CDS             complement(41097..41267)
                     /locus_tag="PC472_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726268.1"
                     /translation="MSALLYGVCNKPILISIVTNMQEFKIGVSMNYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            complement(41664..41876)
                     /locus_tag="PC472_000920"
     CDS             complement(41664..41876)
                     /locus_tag="PC472_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726269.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAVDIAKVFDTTVENLFEFIEEE"
     gene            complement(41877..42293)
                     /locus_tag="PC472_000921"
     CDS             complement(41877..42293)
                     /locus_tag="PC472_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726270.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLSTKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(42361..42774)
                     /locus_tag="PC472_000922"
     CDS             complement(42361..42774)
                     /locus_tag="PC472_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726271.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKYGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(43608..44237)
                     /locus_tag="PC472_000923"
     CDS             complement(43608..44237)
                     /locus_tag="PC472_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2726272.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(44316..45278)
                     /locus_tag="PC472_000924"
     CDS             complement(44316..45278)
                     /locus_tag="PC472_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2726273.1"
                     /translation="MAKIDNMMSILWILNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(45958..49563)
                     /gene="cbpA"
                     /locus_tag="PC472_000925"
     CDS             complement(45958..49563)
                     /gene="cbpA"
                     /locus_tag="PC472_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF2726274.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAEGDANAGNAGGDANAGNAGGDANAGNAGGD
                     TNAGNPEGDANAGNAGGDANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEGDMNAL
                     VVEEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIV
                     IPKDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGM
                     RAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRG
                     SLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGD
                     ITINGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSY
                     GGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSL
                     KATTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYG
                     APSYTDDQGVLYLWFSEQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQ
                     YEEFTIDDTPPYLIIKRYDGLPFEEDVEKSFLDWILKDGGIPIITPKGEKLTDKKFMT
                     ISSQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYK
                     AEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYIN
                     GKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIE
                     GSEDEKKLKLDVTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGE
                     WKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGS
                     GGYDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNG
                     GNSKPDPDNGGNGKPDPDNGGNNKPDPDNGGNSKPDPDNGGNSKPDIDIDIDTDGDGK
                     PNINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKPDVNIDIDGDGKPDINIDTDGN
                     GKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            49832..50403
                     /locus_tag="PC472_000926"
                     /pseudo
     CDS             49832..50403
                     /locus_tag="PC472_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(51415..52077)
                     /locus_tag="PC472_000927"
     CDS             complement(51415..52077)
                     /locus_tag="PC472_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726275.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(52094..54649)
                     /locus_tag="PC472_000928"
     CDS             complement(52094..54649)
                     /locus_tag="PC472_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2726276.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKSDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEALYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(54882..55076)
                     /locus_tag="PC472_000929"
     CDS             complement(54882..55076)
                     /locus_tag="PC472_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2726277.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(55088..55267)
                     /locus_tag="PC472_000930"
     CDS             complement(55088..55267)
                     /locus_tag="PC472_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726278.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(55272..55529)
                     /locus_tag="PC472_000931"
     CDS             complement(55272..55529)
                     /locus_tag="PC472_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726279.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLENLEYTKEQSSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEGDKFIMAIYNI"
     gene            complement(55502..55669)
                     /locus_tag="PC472_000932"
     CDS             complement(55502..55669)
                     /locus_tag="PC472_000932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726280.1"
                     /translation="MKIKKITITMVIAISLTLGLITGCTNKVSDTNEKIYTIEQISSI
                     KKTCQRKYQNQ"
     gene            complement(55790..57183)
                     /locus_tag="PC472_000933"
                     /pseudo
     CDS             complement(55790..57183)
                     /locus_tag="PC472_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(57177..57852)
                     /locus_tag="PC472_000934"
                     /pseudo
     CDS             complement(57177..57852)
                     /locus_tag="PC472_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(58492..>59020)
                     /locus_tag="PC472_000935"
     CDS             complement(58492..>59020)
                     /locus_tag="PC472_000935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726281.1"
                     /translation="HSYEILIRKLVYNLLPKCKKINSKIIEREIKNNLVIDCSNILKK
                     YTNKIIINAHKVYINNEKIITLSNEIDLIAVVTKKLFIIECKDIYYKFTPYGFKCDIN
                     KTYSFISKMEKKYKSVLENKQNLEIKFGCEIEEIQPIIVFKTYNMAINSKLNNNRIKI
                     INFNGLEKLIRDTKN"
BASE COUNT        19570 a         9929 c         6336 g        23185 t
ORIGIN      
        1 taactcatga ctaaagtcac gagtgtgcgt agctattttt taatcaaagt gtacaacttc
       61 tatacctttt tctattaaat tcaatgtaac tcttttatat aacttgtcta atggctctac
      121 atttggagta gtaactaaca aacatttctt tccatatttt cctattattc taggtaattc
      181 acttaaagtt ccttgtccaa aatttattct tgatggttga taaaagttaa tcatattttt
      241 cctcccttaa attattgcct tcatattcaa attatacata tcataggatt tttaaggcaa
      301 taccttcact gttaactctt atttactaaa gcttatggta attattagac aatcaatgtt
      361 ttattattac catatcatta tgtaaatttt attaagtacc catgttataa ttttataatc
      421 aatcttattg tataattgac ttaataataa aaatagagta tatacacttt tttatgacaa
      481 atagcttaaa ggagaattaa attatgaaat atttgataga cagtgctaat cttgatgaaa
      541 ttcaaaagtg tctaaagtat ggatttaagg gaataaccgc taatccatct atgtatctta
      601 aagagaaagt aaacttccat gaatttattg agacatgtgc taatttaaat ccagaaatat
      661 taactgctga agtcattgga aataatttat acgaattaat taattcatgt gataagatat
      721 taaatataaa taaaaacact ataataaaaa taaatttctc tgaagttggc ttagaactta
      781 taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac cttgattttc aatgtaaatc
      841 aagcatcatt agctataaat gcaggtgctg attacctatt cccttttata ggtagaaatg
      901 atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat atgtaacctt atatcaataa
      961 atggatataa tactaaagtt gttgcagctt ctattaaaaa tgtattccac ttaactcaag
     1021 catctctttg tggatgtgat tatgcagctg taacatatga tttatttaaa aaagcttctt
     1081 ttaaccagct tacaatagat ggtgccaata cttttgaaag agattggtcc aaggttaata
     1141 aatagtaact aagatttagg agggtttgca ttgaacaaaa gattattaga tattgctgaa
     1201 ttattactca acagctcaga ctatattact gtagatacta ttgccgaaga attaaaagtt
     1261 agtaataaaa ctattcgtaa tgaccttgtt attttagatg aatggctttt agagtttaat
     1321 ttatccctag ataaaaaaac tggttcaggg gttattattc taggtaatga agatataaaa
     1381 cttaaagtta ttagagatat caatgataaa tcaaattgta tatatgcata ctctcctgaa
     1441 gatagaaaaa ggtatattct aagtaaatta tttattaaaa attctaagtt taggattcgt
     1501 gatatttgca atgaactaca tgttagtaga gctactgtgc ataaagactt ggtagttatt
     1561 caaaattttt tgcaaaactt taaagtgact ctagttagaa aaaccaataa tggagtatat
     1621 ttagagggaa aagagaaaga tattcgaaaa gcaatttttg aacttgctac ggttaataaa
     1681 agttatactg aacttaaaga aatattattc tcgaaatcta atgaatgcaa agatacccca
     1741 tcaacaaaaa tatttaagga gctatttaac tatgattttg agaaattatc tcaaatcact
     1801 ctacacacct tacaagtaga aaaaagtaac cttagtgatg agtattatat aaacttctta
     1861 atacatatag ctatatgtat taaaagaatt tcaattaaaa gatatattaa cttatctgaa
     1921 ggttttttta gagaattatt agaacatgaa tactttgaaa aatcaaaaat tttatgtgct
     1981 aacctaagtg aagcttttga cgtagaattt attgatgaag aggtctgtta tatccttctt
     2041 catatagaag gattacttaa atcaataaag aaaactacta ctcttgaacc tcttaaaaat
     2101 gaagaaatta cagagtataa tctatctgaa gcaatagctg ttcattgggg aagtatacta
     2161 aatttaaact taaaggatga tttaatatta ataaagtctc ttgctaatca ccttaaatca
     2221 gttctacaca gaataaacta tgggtttact attacaaatc ccatattaaa tgatataaag
     2281 agaacatttc cttatactta taaggctgct aaagagtcta atactgttat caaacagttg
     2341 atgaattatg aagtcaatga tgacgaaata ggatatcttg ctttatattt aatatcagca
     2401 atagatagaa gcaaatctcc ccttaatact attttaatat gtcactgtag tgatggtgta
     2461 tctaatttat tagtccaaaa gttaagtttt gaatttaatc agattaatat agttaaatct
     2521 atacctctat cttctatttc atttacgaac tttgatgatg tggacttaat actaaccact
     2581 gctcctgtag actttgaaca taatgctgaa ctgataaata taaatgctct tttaagtaag
     2641 aatgatattt ctagacttag tacaattata aaaaaactat attctgaaaa aaataaaata
     2701 ttatcttata aataaacaaa aataggcttg tctcaacata gagataaacc tatttttcat
     2761 tgtctaaatg ataaaaatat atttgatttc atagaaaaag tgcctcatga actaaaaaat
     2821 tcattttgag acacctccat tttttatatt taatcttttt atttaagctt tttatttaag
     2881 cttttatatt ttttaaactt ttatatttaa agtaaatttt attattttta ttgagcacaa
     2941 gcattattta tattattaat ttcttcagta ttcttaccaa actttgtagt tacaaatcct
     3001 attgttttta ttatgagtaa taaagatatc aatgaatata tagatatcat aaaattctta
     3061 cttattattc cagacgctac tattataaat ccatctagca taaataatat aactggcaac
     3121 ttcagaaact taattacctt attaataagc attgcaacta aatcagttcc accagtacta
     3181 cagcccctta ttattgctat ccctatagca gttcctacca ataacccacc tatcactcct
     3241 gctactaata gattgtcagt tatgtgagtt tgtggtatta cttttaagaa taatgggccc
     3301 ataagagtta tatataatgt ttttattcca aaacctttcc ctaaaaataa taatcccatt
     3361 actaaaagtg gcatagatat tcctaatgat atattctcaa caggcattga taagaatgtg
     3421 ctaagtacga ctgataatcc tgttatacct cctggagcta atgtatgttc tgcaaagaaa
     3481 aaattaactg ctaatgccat taaaaatgct gatattgtta tcagtaaata atcttttatc
     3541 atttcttttt tcatttaata acctcctatg attatattgt ttcagtataa aaaatcaatt
     3601 aaattagtct agcttcctgt agtttttcat aaaattaatg cttccttaaa acattaagaa
     3661 agcatctctt ttatcatagt ttattatctt attctaaacc aaactttgta gctgcttcat
     3721 ctatagcttt tctacaattc ttaacacctg ctgttatacc atctttatcc ttgaatattg
     3781 ctgaagataa tattacaacg tctgggttac attctattaa tggctccata ttgtctgatc
     3841 ttagtccacc atctattgct aattcacact taggattctt ttcgtctatc atttttctag
     3901 ctctttttac taaatcaaca gcactctttc tccatcccca gttatctttt ccatcagttt
     3961 catcaacacc atgaacgact atttgcaatc tgtctatatc atatatagct tcatcaacaa
     4021 aacataatgg tgtgtaacat ccaattgtta atcctacttt cataccaaat tctctacagt
     4081 aatttattat gtatgcaaga ggagcaccta aaaaatgttc tgctggtatt atcaacaagt
     4141 tacatcctgc tgctgctatt ttttctataa ataatctatc acaatctctt gtgtatatat
     4201 ggcattctat tggtttatct gtcactggtc ttattgcttc tattatttga tgaccaccca
     4261 taagttgcat attttttaag tcatgcatat ctgctgcatc tgaatgtacg tagtctacac
     4321 ctgcatccat tgcttcttta actacggctg atatatgacc gtaatttaca tgtgctaatc
     4381 ctgctgctat ttttatgtgt ttcatatttg tatacctcca tattttactt taattagtat
     4441 taaatattag tttttgatat ttttttaata ttttttaatc cagtattact tttatttagc
     4501 attactttta ttgctgtata tatttctttt atgaacttat tctgcttttg ttaactgtga
     4561 acttattttg ctttgtaaat taatattaca ctgatatatt ttttttcaca acactttgac
     4621 tactccttaa aagtaccttt atattgtggt aaattataga cattaattgt atataaaaag
     4681 ttagtttttt accacattct tatggcattt attgtctata ctaactcaat tgatatagct
     4741 agttcattta atatataatc agactataaa tataaattta ctcgataaaa agtaccattc
     4801 ataacaatat taatgaaagg gagatgtaaa atgagaaact gtattattgc ccaatctgga
     4861 ggccctacag ctgttatcaa tgcttctgca gttggtattt atgaaaaaaa tttagaaact
     4921 aaatactttg ataaagtata ttttggtatc aatgggattg aaggtatatt aaacaaaaat
     4981 ataatagatt cagactcctt aaacaaagat aatgtattaa atttaaagca aacaccttcg
     5041 tctggtcttg gttcttgtag atacaaactg gctgattata aaataaattc ttatgattat
     5101 gatagagtta taaatatact agatgaatat aaaatagata cattatttta tataggcgga
     5161 aatgattcta tggacacagt gatgaaattt tctcaatact gcaaaaacac tacttgtaaa
     5221 ataaaattta taggtattcc taaaacaata gataatgatt tattaggaac tgacaactgt
     5281 cctggatttg gttctgctgc aaagcttatt tctacactaa taatggaaac atacttagat
     5341 ttttctatat acaaaaataa tggtatattt atagtagaaa ctatgggtag agatactgga
     5401 tggttagcag cttcttcttc actagctaga cttaatgata ctccacttgt tgatttaata
     5461 taccttccag aaattgattt ctatgaagat aagtttatag aagatgttag aaaaatattt
     5521 aaagaaaaaa ataaggctta tatagttgca tcagaaggta taagggataa atacggcaac
     5581 tttatatgcg aacaaaaaac ttctgctcat gataaatttg gtcattccca attaggtgga
     5641 gtaagcaact atctaaagaa tcttatagta tcatcatcaa ttacaacaag agtaaaaacc
     5701 ttagaacttg gtgtaataca aagatgtgct atgcatttag cttcagatac tgatttaaaa
     5761 caagcagctg aagttggtgc atatgggata gaactatcta aaaaaggata tacaggagtt
     5821 atggctatca ttaatagaac atgcaataat ccttataagt atgaagttag tcatactgat
     5881 atttctaaaa tagctaacaa gacaaaatat tttcctaaag agtggataaa tgaagaatgt
     5941 aattatataa ctaatgaagc cctaaattat ctatatccac ttattcaagg taaaccagat
     6001 atcaaatata aaaatggact accttatttt tcaattattt actaggaaat tattataatt
     6061 tgatatacac tcaattctat cgctcttaaa tacctaagtg gctgttaaat aaaattataa
     6121 attgttatat gacagtcact taatcgttta attatttaac tgtttagttt tttaattttt
     6181 taatcttttt aatactaata caatttgttg taataatatc tcacacccat tttcattttg
     6241 gatagattat aattagttag acaataaata ttttgtcatg atattattat atatagtgat
     6301 acaaatagaa atacttttat catggctaga aaaacaaaga ttacacaaag gatttaaaag
     6361 tcaaacaact tcaattaaaa taaatggtta aaataacttc taaatcaact acataaacaa
     6421 taaaagaaca taaagtagtt aaatctacaa ttataaaatt agtaagacta taataactta
     6481 aataaaatta aagttaacaa agactattaa agttagcaaa tattcctcta cctaaataat
     6541 tgctttttat aaagagttat ttattttaat tgaatttaat tgaaatacga ttttaactag
     6601 aacaatagat taatgtaata tttaatataa aataattata ataattgaaa aggaagtaat
     6661 ctatgatgaa aatagcagtc ataacaaccg aatttttaaa agagtttgta gataattcta
     6721 taaaaaagtt aaatattaat gcagaaattg aaatatatat ctatagagat ttttctcatg
     6781 ttggagattt atatttggaa atagaggata ggtttgatgg atttgcagtt agtggcccta
     6841 tccccaaaaa agctattaca aaaaaggctg gaactattaa aaaacctcta gttgattttg
     6901 gtactgactt acaaagttac tatgatatgt ttctaaagct tatctttaaa tataatacat
     6961 tagatttttc tagagcgtac tttgacttaa cagactgggc agataatcca aaagatatat
     7021 catattattt atcaaatggc acatttggag acttaatgga tagtattgat gaaaatgcat
     7081 ctaaaatgag tttatctgac atagcaaaac ttgaaaaaaa gattgcaaaa aaacatatta
     7141 atctatggaa tgaagggaaa atcgattttt ctactactcg ttttagtagt attgttccta
     7201 tgttacaaaa tgcaggagta aactttcatt tcatataccc tgatatagat ttattaaaaa
     7261 atacttttaa taacttaata aaagatattc atttacatag aatgaaggga aatcaaccaa
     7321 gtgtaatata tataacagtt aaagatacaa attatgatga ctctactcta gaccaagttt
     7381 ataaaatatt aaatagtatt aaaaaagata aattaaccga ttttataatt gaaaaaaata
     7441 gtaattcttt taaaattttc actaaatgtg aaacgattaa atcattaact aaaaatttta
     7501 ctgcatgtta tataaaagaa tctattgaaa atagtacaaa tattaaagta tgtgttggat
     7561 atggaattgg taatgatata aagcaagcta tgtctagtgc cataagtgct aataaggaat
     7621 ctaaaataaa tcatttaaat tacagtttct tagttaacga aaattatgag acaataggac
     7681 ctttactgtg tgataagtta cttactgttt caaataaagt aactccacat ataaatacaa
     7741 ttgccaaaag ttctaaattg tctactctta ctattcaaaa gattatatct gttactgaaa
     7801 ttctacaaag agatgaagtt actcctaagg aattatcttc atgtttaaat gtaacagtta
     7861 gaactgctaa tagatttcta agtagtctct taaagtctgg aaacgctact atattatatg
     7921 aaaaacaaaa ctcaacaaaa ggtagacctg aaagggtata caaattattt atatcagaaa
     7981 ataaatatta aaatccaaaa tgcataaaaa tattttatca tctaatatta agtaaaatta
     8041 tatatgactt tctaaaaggt atatctttgc atctttttaa gatatacctt ttatgatatt
     8101 taataatatt ttatgttatt atctactaaa attattttct aaccatttta ctgcacaatg
     8161 agcaaataaa gctgaaccat aaactaatcc ctcttcatta aaaattgctt taggattatg
     8221 cataccaaaa ttatatccct gactaatact tcccattcca atccataaaa atactcctgg
     8281 aacccttagt aaaatctctg aaaaatcttc tgaacccatt gcagatggaa agtctattac
     8341 attttcattt cctataactt ctttaacata agatacaatc tcatctccta catccaaatc
     8401 atttataact gcaggagtcc ccattttaaa ttccacttta gcatttcctc caaatgattt
     8461 agctattcct gtagatattt caataattct attttttaat ttttctctaa caaattggtc
     8521 aaatgttctt aaagttcctg acatagatgc tttatttggt atagcatttg gtacgtttcc
     8581 tgcattaaac ataccaatag ttaaaactac aggttctttc tgaggttttt ctcttgaaag
     8641 tatagtgttt aatgctattt gtatatgcga cattatgttt attgggtcca ctccaaattc
     8701 agggcttgct ccatgagttg cttttccttc tactgttatt tcaaatatat ccgcagatgc
     8761 catagctgga ccacgtgaat aagctacttg acctgtgtta agaaatttac ccaccatact
     8821 gtgaaatgcc atagctgcat cgacctgagg attttctaat ataccatcat caatcattga
     8881 ttttgcacct ctggtagtct cttcatctgg ttcaaataca aactttactc tacctttaag
     8941 taagtgttta ttatccataa gtattttagc tgctcctaag agcattgctg tgtgattatc
     9001 gtgtccacaa ctatgagatg catctttatt cttactttta aaaggtaaat ccgtctcctc
     9061 cataatagga agtgcatcca tatctgccct taataataag gtctttccac cattttcact
     9121 gtctaaaaca gctactattc cattattaga cgtcaatttc ggagaatatc ccattttttc
     9181 taaagtactg gtaacaaagg ttactgtttt cggtatattg caaccaattt cgggattttc
     9241 atgtaaatat cttctccatc taataatttc cccttctatt gactgcgctt ctttaaaaaa
     9301 atttttcata ttccttcctc ctaatcagat agtcagcaaa atctcacttt cttttagtaa
     9361 tgaaatgatg ctgatataaa aattataatt ttatatatta aatttcatgt ctttttaagg
     9421 agttggaata tcatctggaa taggacattt atatgtttta ccattcatag atttatcaaa
     9481 ttcttctttt gcctttttag ctatctctgg attatttaat attttagctc caaacatagc
     9541 cataatcttt gatgcatata tcataccttt ttctcctata gaacttcctg cacaagatgt
     9601 aaactgccag ctatgacctg gtgctcctag attcgtacac cctgtaaaaa agtatgctgt
     9661 cggaactata tgcattacat ctccaatatc agtagaacca taactattaa agtttgtaac
     9721 atttcctcca ccataatata gatgagttcc ttctggcaaa ttatatttac ttatcatagc
     9781 cttatattgt tgtgggcttt gtctatctaa ttcagctgca aaatccaatt cctctcgagt
     9841 ccaaggctct tgtttaatct cattcataca ttctgataca agatttgcta aaacatgatt
     9901 gtttaaagta ttataacaac ctcctaaaaa ctctacttca acttcagttt cagtcatcat
     9961 tgcagctcct tttgctacct ttacaagtct ttcatatgta ctttctacag cttctctact
    10021 taaagctctt gtataatacc atactgatgc tttatcagga actatgttgg gtgctactcc
    10081 tccatctgta attgtataat gaatccttac atcagaagta acatgttctc tcaaataatt
    10141 agctcccaca ttagtaagtt ccacagcatc tagtgcactt cttccatttt gagggtctga
    10201 gcctgcatgc gcagtctttc ccttaaagtg aaactttaca gagttaacag ctgttgaagt
    10261 accaattgta gcttcactaa ttttatttgg atgaaaattt atagctaagt ctaacccttc
    10321 aaacgctcca cctctagcca taaatccttt acctgttaat tgttcttctc ctggacatgc
    10381 ataaaatact atagtcccac ttaagttatt ttctatcatc tctcttttaa gccctactac
    10441 tgcccctaca tgtgctgtac ataataaatt atgcccacat ccatgaccat atgcacccaa
    10501 ttcaaatgct tcttgctttg ttgaaacttt ttgatttaaa ccaggtaatg catcaaattc
    10561 tcccataaaa cctataactg gtttacctga accaaaacta gctctaatag ccgtggctaa
    10621 acctcctaca cctacttcca catcaaatcc ttcacttctt agaaagtttg ctgtattttc
    10681 acatgcttta aattctttaa atgctttttc tggattttcc catattttct tacttaaatc
    10741 atcaagtttg aatctttttt tatctatctc atttatagat atacttccta tcatttccta
    10801 ttcctcctat aagtatattt actatcaacc aaatataatt ggacctatta tactagcaaa
    10861 tgccactgat gcaatggtta cagtaacaaa tcctcctata atcatctttg gtaataaata
    10921 atctgttgcc ctttgttttt cttcttcagt tccttccata gcatcaatac cttcgttgga
    10981 aattatctgt gttgcaggat aagctagcat acaagttaca ccaattgcag ctgacattct
    11041 tggagaatat cccaatagtt ttcctacaat tgcagtaaat aatattataa atacagagcc
    11101 tacaacaagt attcctatag ttggaacaat catcccaaac aatgctgttg gagttacctt
    11161 agcaagtcct ccaggtaaca tcaacataca accaaacatt ataattccat tagattgaga
    11221 tttatctaaa attccttttt ctaaaaaacc tattcgtgta aaaatcaacc ctactaacaa
    11281 acatgctata ttaggattta aataatagtt tgcaggggta aatcctggta ttaaagtagc
    11341 caatcctaca aaattaccaa tcatagcaac aaatgaaagc tttgcaagat acactagagt
    11401 agatgatttt ttacctaaat cttctttaaa aattctcata cttggaagtt taaaattact
    11461 ttttgactca tctttcaaaa aatccccatt atctctttta aattttaatt ctttatttat
    11521 acaatatgta gttatcggaa tgccaaagaa cttttgaaat gataatacta gaattacaaa
    11581 tgcagcaagt tcaggtctat ttgcagcttc tgcaacagtt gttacgataa taccagcaac
    11641 agtagaaccc gctattggag gtgctgctat taaagcatac tctctaccaa ataacatact
    11701 acccactgtt aaacagccaa cccctattcc tacaatacct actaatgcaa ttacaaccgt
    11761 tttccattca tgacataagt cttcaagatt tataagtgtt cctaagttag ttattaataa
    11821 agctatacca aagttgctca ttgcagctaa tatccctgat gctcctgtaa tgtcttttgg
    11881 taaaatattt gtccaaaatc ctattaaaaa tataatacat acaaataaca atgttgaaat
    11941 tgcacctttt gttttcttag ataaaaactc actaaaagca taaatcatta cacatacagt
    12001 aaagcaagta agtggattcc aaaaattttc cattgttgtt tcctcctaaa tataataatt
    12061 ttttatcaat aaaacaataa ttataaaatg tatattatgc agttattctt aactacatat
    12121 ggtatagctt gatatttttt gatgatatta tatgcgtcct aaattattcc taaaatatct
    12181 aaataaatat aactagagtt aactctctaa atattaacta tagttatcaa cttactagat
    12241 ttctttttga tttttacttt ctacataatt ttacgagtta gaattgtact tcaactactc
    12301 tctatattag tatacatgtt ggaagactta tatttattta aatattagat atagaataca
    12361 tttttgcaaa cgtcttaaat tattcttaaa atatctaaaa cgaaataata atatacccat
    12421 taaaccaata ttatcagaat atacttcact ttaagtcttc tttatataga aattttatct
    12481 attagtgcat cccctccttt gtaaattaga ctatttctaa acgaatttag atatcttatt
    12541 cctaggaact attactagaa acaaaaatta tttattatat taaatcaatg ataagccata
    12601 ctttaaaatt ctttctatac aagtatgatt tatcatataa tgacatctat acctttatct
    12661 ttatagtagt cctaaattat tcttaaattt acaataaaaa taatttcact atttataaaa
    12721 atacttgttt ttatattgtc aaactattct taatatagat tataatttta aatcttttct
    12781 ttgtcaatat attctgacat ttttccagaa aatttataaa gttatatagt gagtaaatca
    12841 tactattttt atataatgag taaattatac tatgaatatg tctaaatatt tacttaagta
    12901 taaacataaa aaatgtgtat tctccttttt atagtaattt tagttgccat tacttttaag
    12961 aagaatacac actttacttt aaagacccta tactctattt taaatgcttt actagacact
    13021 aattaacatt agttcctaac ttctatttat cttattccta ccctactact atatccgtag
    13081 aaaaatctaa aacttctcca ttagatttta ttacttcttt gtaccaatca aatgattttt
    13141 tcttatatct cttaaaactt cctttgcctt catcatctaa gtcaacatag ataaatccat
    13201 atctcttact catctgacct gtagttgcac tgactaaatc aattggtccc cacatcaaat
    13261 atccaaaaca ttcaacatta tcctcttcaa ttgcagactt catttgagat agatgcttac
    13321 ttaaatattc aattctataa tcatcattta tagttccatc ttcttcgatt ttatcatatg
    13381 cccctaaacc attttctgta attaagatag gtaattgata ctttctgtaa atatagttca
    13441 aggtaaatct taaacctata gggtctattg cccaccccca cttactttgt tccaaatatg
    13501 gattttgaat cccaccaaat aaagatttat cacctgataa attttctttt ggagtagata
    13561 cacttgaaaa ataataatta agtcctataa aatctaactt accttcttta aatgcttttt
    13621 tgtcttcatc agtcacttca atagttatat tatttttttc atattctttt aatttatatt
    13681 gtggaaactt cccattgcac attgcatcaa tttgataata atcacggtca ttatctaaaa
    13741 atgcatttaa cacattacta ggattacaat tgtatggata aattggattt agtccaaata
    13801 cacatccaac tttattgttg ggattgattt ctctagccaa ctttgcaacc tttacacccg
    13861 ccaaagtcat attataccct acatttgcca atgtttgaac cttattttct atttcactat
    13921 acttaatacc tgctatcata taagtaaaaa tatccgagtg ctctgtttga gggtcaatat
    13981 ggttcatctc attaaatgta acccagtact ttaccttatc atcaaatctt cttattacag
    14041 tctcactgta ctttaaatac aaatctataa gttttctgtt attccatgaa ccatatttat
    14101 gtactaaatt cataggtaat tcaaaatgat atagtgttac aataggttct atattatttt
    14161 ctattaatgt atctatcaga ccttcatagt atttaatccc taattcatta ggattttcat
    14221 catctccatt tggaaaaatt cttgaccaat ctatagatat tcttaatgca ctaaatccca
    14281 tttctttaaa taaagctata tcttctttat atctattata aaaatctatt ccattatgag
    14341 atggataatc caatttttct tctattttat cagtaattac tcttggcgtt tcataagagc
    14401 ccttagttac aaaatccata attgcaggtc ctttgttatc actatcccat gaaccttcac
    14461 attgatgggc tgcaatactt cctccccaga aaaatgtatc tttaatattc atgaattttc
    14521 cctcctaaaa tttatactat tacagttaat aattcttctg attcatcatt tgatgagatt
    14581 acatctaaat aatcgctagt atttgtaact acagttatta tagtagaatc aaagccttcc
    14641 tctataattt tatcagattc aaatactgct aaagcatctc cttttttaac cctttgattt
    14701 tgtgttacaa gactttcaaa gaattttcca tctaagttta cagtatctat cccaatatga
    14761 attaatattt ctaatccttc atcagtcact ataccatatg catgtttagt tggaaatact
    14821 atagttatag ttccatctac aggagatata acagttgatt ttgattcact aggtataact
    14881 ccaacccctt tacctaatgc tccagatgaa aatgctttgt cattaacttc cccaagtgca
    14941 attacctgac cttttgcaac atttccaata ctaatagttc ttgaattgtt ctcgttgatt
    15001 attagttcta attctttttc attatcaccc ttaacttctt ctttttcatc tgaaggtatt
    15061 cctagtaaat aggaagctgc tgttgctgct acaaatgata ctactacact tattatcata
    15121 aatgtaaagt tagatacctc tcctccaaca agatatgttg gtaatgcagc taatccccaa
    15181 accattccat atgttttaac ttgagctaaa cctgctatta aaccacctat acctccacct
    15241 atcattactg ctacaaatgg tcttctatat ttcataaaca caccaaacac tgaaggttct
    15301 gtaactccca taagtgcact aaaacttact gtaccaaata attgtttttg ttctttattc
    15361 ctagttttta agaaatatcc aaacattgca ccacaaactg caatatcaga tattgttgca
    15421 gcgcccataa aaattgggtc ataccccaat gttgtcacag aatttaaaga tataggcata
    15481 gtaaagtttg ctgcaccaaa gactattaag agtggttgta aagctgcata cagtgcaaca
    15541 acactccaat tacccaatgt atcaattaaa aacatacatg catagtttaa tccatcacca
    15601 atccacatac caataggtcc aaatacagtt aatgtaattg atagagttat tattaaagat
    15661 aatactggaa ctaaaaaata gtgtaatgct ttaggtaata tttttttcaa accttcaatt
    15721 ataattccca ttgcccatac tcctaaaagt attggaataa aactgtttgc ataagttgta
    15781 gcctgtaaat ttataccaaa taaacttaat ccttctacac catctattga tgaagataat
    15841 aacgtaactg ctaacactac tgctatatag ggatttacat taagtctttt tgctgatgct
    15901 attgcaatta atactggtaa gaagtgaaat gtagcacttc ttaatgtatc taatatttga
    15961 tatgtaggac tgtcactctg aagtatgcca gacatattta aaagtgctaa catacttgca
    16021 agtataccag ctgcaagtag tacttcaatt actgaagtca tagtctctga gactaccaca
    16081 aagatggaat taaatatacc tttgatacct ctaattccct tttttctttc actttttata
    16141 cttgattcac ttgaacttaa acctatcata gacataaact cgttatatac atctacaaca
    16201 tgagtaccta ttataatttg ataagcaaca tcattagaaa ctacatcaat tactcctttt
    16261 aattttttaa tttcttctgt ttgagcgata ctcctatcat ttaaagtaaa tcttaaacga
    16321 gtaacacaat gcgctaccga ctttatattt tcttttcccc ctataagttt tattatattt
    16381 tcatttaaat ctttatattt catctgattt tgcctccagt ctttgagtca ccctatttat
    16441 atgcaacatt aaataagttt cttcacttat agatatatcg acattaaatt gtgattttat
    16501 atattttctt atcttttgaa cacttccaaa tgactttgga taaagttgac ttacttgttt
    16561 atataaatct atagaattat cttcatctat tgaagcttca tccgacataa ttctttggat
    16621 aaagtactgt agatgagtag taaatctcat ataatttgtt gaagtttcat ctagctctac
    16681 attaaaatga tattttatta tagaaactat atctgctaaa gttctcaact ctacaattgt
    16741 actatcatga gactctttat ttacctccat atttacaaaa tgaagtgcta ttgatactgc
    16801 ctcatcatca ggaagattaa gtcccttact atcaccaatt aattttagtg catgcattcc
    16861 aatttcaaaa tgctttggat aaaatctttt aacattccat accaaaggac tttttataaa
    16921 ttgattcttt tcacatcttt taagtaaaaa ctctaaatgg tctagtaagg ttaagcttag
    16981 ataatcactt gactttatat ttaaagcttt ctcaccatat tcaattattt cattaataag
    17041 aactatatta ttggagtctg agttttgtag tagataacta aagtgttcta gcatatcgta
    17101 tgaatctagt atatatgttc tctctatttc attttcattt attacttgtc ccactctttt
    17161 tttaaagcca attcctttaa caaatacaat aatttcattt ttacctcttt ttactaaggc
    17221 tacattatta tttaaaatct gaattatttt catatattcc tcccttcaat aaaaagaact
    17281 caagcatata tgtatagagt atccctctat ttttcacata tatacttgag ttctgcctgc
    17341 taatcagtaa catactcaaa attatgacta cgtcgccata attaaaattc ttttttgttt
    17401 aatttcatat tctatatgtt atactatcca tataaatctg tcaacggttt cctagatatt
    17461 tttttattct ataactacaa gctctgttat gcgattatct gacaaggttt atatactaaa
    17521 ttgatactat tttaccgtca ctaatattta taatattgtc tgaaccatta tctattttac
    17581 tattatgaat tatcatagta atgcttttaa atataatgaa ctgtatgtta acaaataaat
    17641 tcttaagata tattaacata taaagtctta agtttagttt tatttaatca ctattaagat
    17701 atattaatag gtaaagcttc aaatttagtt tcatttaaat gctagtaaga tatactaata
    17761 tataaatact taaatttagt tttatttaaa cgctctagtg atatttttaa ataaatttta
    17821 ataaaaatca ttctattttg tcataaatca tttatctggc ttatatttaa gatatattgg
    17881 tggatacttt acacttttta attcctcatt acttcttaaa agtttattat tattttccat
    17941 atcataaatt tgtaacatta ctttatttac atatagagca taataattat cactataaaa
    18001 atattgatac tcatatcgag ggtcatcttt gtaatacact ttgtatagta ttccttcgta
    18061 acttgccttc ttggcttttt cttttgtaat actttttata ttatcttttg aaatatcttg
    18121 ttttaccata tacttatcaa tatatgtttc agcatctttc catggtttaa tgcagttttt
    18181 atatacaaca aaccctgtta cacttataat acctataatt aatactataa aaataacttt
    18241 atattttttc atattgatcc tccttcattt attatttaaa actcgatata atgcactaaa
    18301 aaaaggatgt tatctttttt attaaaatat aacatccctc atatttagca tcaatatata
    18361 tgccccaact ggtaggttaa aagaccccac tgtcagtact ttgattaaat tacatgtatg
    18421 tatatatatt tttaacaaat ctattatatg tcaacttgtc tactattaca tataatctgt
    18481 aatattacat ctgctgcttt ttttgagcct cctgcattat aaaagctttt tgatagttta
    18541 gttgcatttt ctttatattc attgtcattt ataacttgaa atactgcttt tttaatactt
    18601 tctgacttat cctcttttaa cataattcct gctcccaaat gaacaactct cttagctacc
    18661 attctctgct ccgaatgttg aggaaataat accataggta ctccataata taaactttca
    18721 ttaacactat tcatcccaga atgagtaaca aacacatcag ttttttgaag tatagcaatc
    18781 tgttcaacac tatttttgac ctcaaaatta tttggtatat ttcctaaact tttaatatca
    18841 gtacttctac caacagacat aatcacatca acatcagaat ctttaaatgc acttatacag
    18901 tttttataaa agctaacatt tttattatta attgtaccaa gcgaaatata tattagcttt
    18961 ctttttctct ctttaggttc aataatcaac tttgatactg atggacctac aaatgaatac
    19021 ttactagaga aggtttctac cattggttga aattcttttg atgtatatac aatggtatcc
    19081 gtatcattat cattagaaac tatagaaata aaatttttta cttcataccc ttttctttgt
    19141 aaaagcttta tttttttatt aatgcgtctt acaccaaaaa tcatacgtat aatttctata
    19201 aagttttgtt tcatcaactt tgctgtatgt ttattaaatg caaaagttgt tgtagagcat
    19261 acatatggaa tattaagttt ttgtgcaaat aactttcccc aaaaacttaa tgaatctgat
    19321 acaatgcaat ctggattaaa atctttaagt tctctacaaa ctttttcatc taaagaaata
    19381 gtagtatcta ctatcatctc aattaatcca ggaaaatcct taccaatttt tttctcatct
    19441 ccagggagta attctggaag gtatttatca caacaaataa attttgctcc agcaccttca
    19501 attttatctt taaattcatt aaaagaataa tacaatacct catttcctct atccactaat
    19561 tcacgaacta cttcaatagt aggattagta tgaccatatg caggaatgct aaaaaatact
    19621 attttactca tatttcatac ctccttttat aattttttta aatcattctt ctttacgtac
    19681 tactcccaca tgacctataa aatttatcaa atcacgttga tgaacaattc ctaatcctct
    19741 ttcctcatct ccaagtacca ataatttatc tcctgcattt atattaaata cttctcttgc
    19801 ttcttttgga atgacaatct gaccacgttc accaactaca actgctccaa agaaaaactt
    19861 tcctttagga ggtactataa tacctgtttt ttcttccgaa tgattaacta agtcatctag
    19921 tttaacattg tacaattttg caagtttaat gcaactacat atatcaggaa tagattctcc
    19981 actttcccat tttgctattg attgtctaga aacattaagt ttttcagcta attcttcttg
    20041 tgtatactgg ttgatttttc ttagactttt taaattctca ttaatcatat ttttcatacc
    20101 tcctatgttt ataatattgg atttattatt taaattctat caactaatct taacattcat
    20161 atatatattt atatactttt attatataaa tttattattc aggtaacata tttccttaag
    20221 ttatcgtaaa caaaaaaaca agcacaaatc tactaactgt tgttaaagta tttaagttaa
    20281 ggtcatttac attatcaaat atttctacac ctttaaaata acctttatat ttataatctt
    20341 tcataagcaa attattatcc cttctcatat acaagtccag ttgatgtctt gttgctaaat
    20401 ttaatggtaa tcaaattctt taccaataag tataaaaatt actaatgtgt tataattttc
    20461 ttaactacta aattttacat acatttgaat agaatatatc aataaattac tataataaat
    20521 aaaaaattaa gagttaaaat aaaaaatagt tagggagata ttattatgat tttatatttt
    20581 agcggaactg gtaatagccg atatgtggct cgtaaaattg cacaagaatt aaatgatgaa
    20641 ctgatatcac taaatcaact tataaaagag gaaaaaacag atgaattaat atcagcagat
    20701 aaaccattca tatttgtatg tcctacttat gcatggagat tgccaatagt ggttacggat
    20761 tttataaaag aaacaaaatt tctaggaagc aacagagttt actttgtaat gacttgtggg
    20821 ggagatacag caaaatccat aaactatatt caaaaactat gtaaatataa ggaatggcaa
    20881 ttaaaaggta tggcagaaat taaaatgcca gagaattata tagctttatt ttctacaaca
    20941 gacaaagaaa catcaaaaca aatgattaaa gaagcagata aacaaatata taggattatt
    21001 tctgatatac gaaatgaaaa tgaatttgaa actattactc ctagtggctt aggaggtaca
    21061 attaaaagtg gtataataaa tacagttttt tataagacaa ttattagtgc caaaggattt
    21121 cattatactg ataaatgcat tggctgtgga aaatgtgtag agctatgtcc tttaaataat
    21181 attaatttaa aaaacaaaaa accagtttgg aaaaataact gtactcattg tatggcatgt
    21241 atttgtggtt gccccactga agctattgag tataaaaata aaactcaaaa cagagaaaga
    21301 tactatttag aataatatat tcttaatatt taaaccatta taaaatgaac tatacccatt
    21361 gttttataca gttttacata ttttaactgt ctcctaagat gggtatagtt caaaagatag
    21421 tgtctcaaag tgaactttta agttcctaaa tcaatctttt tgtattaaat caagtataat
    21481 ttaatcacct tttcttatat cattttcact aatatgttgt attccaacaa atataagatt
    21541 tattagctct gttagtgact tccattgtga tttttttgaa tcaagataat aataattttt
    21601 cgttccttct ctacgtaaat taacaatctc tgcttctttt aatatcttaa gatgatgaga
    21661 tacagcagga cgagaaagat gtgttttttt tgtgatttct cccactcgta taccattaaa
    21721 atcactctct aagagagcaa ttaaaatcaa ttgtctagtc tcatcaccca tagcagagat
    21781 agctttgcga cattctttaa atttagacgt tatttcttgc attctttgtt gacattcttt
    21841 ttccataaaa tccaccctct cttataagta tctaacagtt gctacttctt tctgtgcagt
    21901 acgtctatat ttaacatctg ggtatccaat tactaatgtt gtaagaacat gattactgcg
    21961 ttttagacct aaaagctttt ttaactttgg cgaattattt gctacatctg aaaaatatcc
    22021 actaaataaa acacctaggc cataagactc tgccattaat gccatatttg atgctgcaag
    22081 tgaaccacta atcttatctt ttgtaattat cattattgca ataggagcat gcttaaagaa
    22141 aaagttatcg tcaatctcaa cttcttttga atatttaata gctatatttg caataggttt
    22201 tatctttcta aaaaatttca ctgcttcgtt ttcataaatt gattttttat tgtctaaaac
    22261 aatatatgat acatcttgtg aatttttagc actaggggta tacctacctg cctcaataat
    22321 ttgctttata atttctgaag aaacttcttt atctttaaat ttacgtatac ttcttctaga
    22381 tttaattgcc attagtaatt catctgaatc tagctttggc ttatttgtaa gttctatagg
    22441 aggttcatca aatcctgtca aggtaatagc ttttgtggga catattgctg cacaatgtcc
    22501 acacattaga caatcttgtt ttttaattac tgacttttta ttttctataa ttatattgtt
    22561 tactggacaa tcatttttac aaagcccaca tccaatgcaa agttctttgt caacttctat
    22621 tatatgttgg tatttcatat tcattcatct acctttcgtt tattagttta aactattata
    22681 acacgtagac attttttata ttcattggta aagaatttga ttaccattaa atttttaatt
    22741 tctctattaa ataaattttt catagaattt taaagtatca tagtttatac ataataaaaa
    22801 agacaagctt aaaacctaag cttgcctttt tcatctttta ttacaaagta aattgcactt
    22861 acttcccata cttcttaaca attttatttt ctaaaatttt atttgtgaat catactattg
    22921 cataaataca atgtaaaata attctgtatt gattttctat agttaaaaaa gttaaaaatt
    22981 atacttatta ttaattttta tactcttctt atttaataca aatttcttat ttaacataga
    23041 tttcttgtct aatataaatc ttcaccattt gaagcaatta ctttcttata ccattcaaaa
    23101 cttttctttt tcattcttct tagacttcca ttaccactat tgtctctatc tacatatata
    23161 aatccataac gcttttccat ttcacctgta ccagcactta ctaaatcaat aggtgcccat
    23221 gtagtataac ctaataaatc gaccccatct atagaaactg catcatgcat agcttttata
    23281 tgttctctta gataatcaat tctgtaatca tcttctacat aaccattttc atcagctata
    23341 tctttagcac ctaatccatt ttctacaata aatagtggct tttcatatct atcatataca
    23401 tgatttagtg taattctcaa acctaatggg tcaattggcc atccccattg actgtatgaa
    23461 agatatggat ttttagcaga tgtaaatatg tttccttcag ccatatcatc tgcattttca
    23521 tcggttctaa cacaacggct attgtaatat gaaaatgaaa taaaatctac tgtatattta
    23581 ttcaataatt caatatcacc aggttccatt tctggtgtaa tacctttttg ctccatatac
    23641 tttaacgcat agttaggata tttaccttta gattgtacat ccacaaagaa gtaattttct
    23701 ctatctaact ttgtagcttc ccatacatca ttaggattac aactataagg atatacactt
    23761 ccagctgcaa acatacatcc aattaagtta tttgaatcaa cttcatgtgc tattttagtt
    23821 gcaattgcac tagctacaag ctcatgatgg gcagcttgat attttatctt ttcaacatct
    23881 tcaccttctt caaagcaaat accagcaccc ataaatggag catgtaaaat catatttatt
    23941 tcattgaaag ttagccagta cttcactaat cctttatatc ttctaaagat aacattgcat
    24001 aatttctcat agaaacctat catttttcga ttacgccatg caccatactt tttaactaaa
    24061 tgcattggac aatcaaagtg tgtaatagtg actaaaggct ctattttata ttttttacac
    24121 tccttaaata tattttcata aaattgaagt cccttttcat ttggtgtttc ttcatcacct
    24181 tttggaaata ttcgactcca tgcaatgctc attctatata ctttaaatcc catttcagca
    24241 aataacttta tatcttcttt ataattatga tacatatcaa taccagtttt agctggataa
    24301 taatgctcat catcaaaatc aaacattttt agtttccctg taataactgc tgttctatct
    24361 tttcctgttg gacatacatc tacatttgcc aatcctcttt cgtcttcatt gtatccacct
    24421 tcacattgat tggccgcagt agctcctccc cataaaaaat cattcttaaa actcatagtc
    24481 aaagcctcct aaaattataa ccaaagcact agattatgct aatacctcaa tctttactaa
    24541 aataagactg taattagatt gtcttctaat tcaatatttt tactttctgt ttctactaca
    24601 tctaaaaatt gtgatgaatt tgtaataaca attggagtta cagtactata ccccaactct
    24661 tttattgcat aaacatcaaa gtttaatata gtttgacctt ttgtaattct atcaccttgt
    24721 tttatacatg cctcaaaacc tcttccctct aattgaatag tatttaatcc aatatgaata
    24781 agcacttcaa cacctgtatc agaaagaatc cctatagcat gtagactagg aaataaggtt
    24841 gtaactaccc catctactgg agcaactact tttccatcac taggtattat tgccactcct
    24901 tgacctagta caccagatgc aaatgctgca tcctctatat cacttaattt taatacttca
    24961 ccttttatag gacttgttac tatttctctt tctaatttag tagacttgac ttcttttatt
    25021 tcttctttac ccttatttct tactttttta gactcttcta ctttctcatc tttatataaa
    25081 atcatagtta atacaaaagc tacgaccatt gatatagcta taccagaaag agctactatt
    25141 aagttaccca ttcccccatc tggttcaatc attgctggaa gttcaaatat tcccattcct
    25201 ccagttataa attttctaag attaaagaat ccatagaaag cgcctccaat accactagca
    25261 atacaactta ttataaatgg tttctttaaa ggcaataata ttccataaat tgctggctct
    25321 gtaactccaa atataccaga tataaagttt ggtattgcca tctcttttag ttttttatct
    25381 ttagtcttga aaaatattgc caacacaact gccgaacttg caaatgtaca ggcaaagaat
    25441 ggcatcataa cattatcata tcctaaagtc ataacattgt ttatatatac aggaataaat
    25501 ccccagtgca taccaaaaat aacaagaatt tgccaagtaa atcctactat agcacctgcc
    25561 aatagtggac taaaatctct tattgaaatt acaatttgtg aaataagtgt agaaccaaat
    25621 gttgctatag gacctataac tataagtgca acaggcactg ttattaataa tgtaagcatt
    25681 ggcacaaaga aaaacttaac taaatctgga acacacttat taaataattt ttcacaccta
    25741 gaagcaaaat acactataaa tataattggt acgactgttc cagtataatc aattgataca
    25801 attggtagtc caaagaaatc aatatatact ggtgatgcaa acattgttcc tttaaataaa
    25861 gtgtacatca catctgctcc tgctgatata ctgctacctt gtatagcagg ataacatact
    25921 gcagctccta gtgctaatcc caacattggt tttaatccaa acttcttagc tgaagtatat
    25981 cctaagaaca aaggaaggaa tgtaaataat gcatctcccg ctgcatttat taccatataa
    26041 ccaccagata catcactata taatcccatt gctacaaata atgcattaaa tcctttcaac
    26101 ataccacaag ctgccattat ccctaacact ggctgaaaaa ttcctgatat tatatctata
    26161 cctttatcta gtatattacc tgatttttta gtttctgaac ttgtacttga atcatctaaa
    26221 ccaattattt caactacatc tttatagaca gcttcaacat gatttcctat aactacttga
    26281 tattgtcctc cacttttcat tacagtaaca ataccttcca tgttttttaa tacattgtca
    26341 tttgctatac tttcatcttt taaagtaaaa cgtaatcttg taatacagtg tactaatcca
    26401 ctaatatttt cttttccacc aacattgttt actatctctt gtgctaattg tttgtatttt
    26461 cccatttttc tcctccatat ttcgtttaaa ttaatttttt gaaatttgaa ggtttggcca
    26521 actcaatgtt accatccaac cataagtata attttgttag tatcactcct ttcaatcatt
    26581 cttaatatac tgctgaaaaa ttatttcact tacatatttg agtctttaac tactttagct
    26641 aaatgtattg ttaaatatat aagctcatca tctgttaata tgtagttata ttgtttttta
    26701 atgaaatttt taattttaac tacacattca aactctttat tatatttaga tttaataatg
    26761 cttagtaatt ctttgtctgt ctcaccttca tgtgcgctat ctaaaagcaa tctttgcgca
    26821 aaaaacttta aatgcattat aaatctataa taaaacactg attcttcatg aaattctatc
    26881 ctaaaatgat acctaactat gctaagtact tcttgtatta gcttcgtaat atcagatgcc
    26941 aaagtatgac cttcacttaa ctgagcattc actatatgaa aggctataaa tccagcctca
    27001 tcttctggta attttatacc tgttttgttt tcaataatat ctagtgcttt cattccaatt
    27061 tcaaactctt ctttataaaa tcttttaatt tcccaaagga ttgcattttt tacatttatt
    27121 ccttctttca tcctttgaat tgcagaatat gtatggtcac ttaaggatat gtaaatactt
    27181 tcatttagct tcttgtttaa ctttatttgt gcgtattcta taatttcact tgataatttc
    27241 atgtgttcta taggaatatt attgagtaat tcttgtagtt tgtttgatat ttctttattt
    27301 gatattctaa atactttatt tactttagtt ttatctatta gattgccttt tgatttttga
    27361 taaccaattc ctttacccat aacgataact tcttcatcat tatcatctaa tgtgattaca
    27421 acattattgt ttaatatctt attaattatc atgattatcc tccttttaag cataaaaaaa
    27481 gcttatccat aataatgggc atactccttc ctaggatact gctattatta atgaataagc
    27541 ttagcttgta cttaaacaat tactacctaa ttccaattaa tatgataaaa catcagacat
    27601 attttgtcaa ctttttattt ccatatactt catcaatctt ttatgttgct ttttgtcagt
    27661 tctaataatt attcttatta atattaactt atttctttgc atcaatataa atacaaaaca
    27721 agttatagtc aataataata aaaattctat taatgtaaat agttaatatt atacttcctc
    27781 tactacatta tctgtacttt atttaacatt tttttcaact ccaaaaacat atgtcataat
    27841 aaatggagca accaatgcta taaatataac tataagtcca tttactatat ttgaaatccc
    27901 cttttcccct gataatccaa gtactgccat tatactattt tgtggaacat ataaagtcac
    27961 atgagtaatt cctgcaaata accctgcgat tcctgctcct atagatgcta acatagcagg
    28021 ttttttaagt ttcaatgtaa ctccataaag agctggttca gttataccca tgatagctga
    28081 tatccatgca gctgatgcta actgtttcat cttactatct ttgcttctta atgctacagc
    28141 taatgtcgcg ccaccttgcg ctaaattagc acaaacttga gatattaata ttagcgattc
    28201 ttgtcctgta gttgcaaatg ctgatatgaa tataggagtc aatgcatgat gcatccctgt
    28261 cataacgata aatggcattg cagctgctaa taaacaaacc attataaatc ctaaccttcc
    28321 ttgtagtaaa tttattacac tagctaatcc ctctcctaag aaatttccta ttggtccaat
    28381 tactattaat gctattggcg cacttattaa taatactaaa gttggattta aaattgtttt
    28441 taacatgcta ggtgttaatt tatcaatgaa tttttctata tatgacatca tccatactgt
    28501 taaaagtgca gggataacag atgctacata tataacacct tgaattggta ctcctataaa
    28561 tgatataggg tccccacttg atacccatgc agagaaattt ggatgtaaaa acacacctac
    28621 tactataact gctattgatg tattaacatt aaatttttta gctgcattta ttgcaagtat
    28681 tataggcata aagtaaaatg ctacatctcc tattatatct aatactctat atgtacttga
    28741 atttgatgat agccaattaa aagtggtgca aagagataat ataactttta tcatcccaga
    28801 tgctgtaagg gctggtatca taggtgtcat acaaccagtt attgtatcta ttatattatt
    28861 taaaatacta ttttttttat tttttatatc gcttttaata tctcctgtgg tatttatccc
    28921 cttatttatt attgcattaa atacaacacc tacctcatta cctattataa cctgatattg
    28981 agttgaagtt gagtttactc caactacctt atctatagat tttaatttat ctgcattgac
    29041 tttcttttca tcttttaaga tgaaccttaa tcgtgtcata caatgagtta agtcaattat
    29101 attatccttt cctccaaggc catcaattat caattgagct acttcttcat atttattctt
    29161 cataccttat cctctctttt aaaataattt ttaatataat tttaatccaa attaatcttt
    29221 ttactccaag cttgtaatag tgtattgcaa tttaataagc tagtatcttc tacaatatgg
    29281 tcacattttc ctagtacttc ttttttcccc acaccaattg atgtcatatt agcagcattt
    29341 atagctttaa tacctgaata agaatcttct attcctacac aatcatattg ttttaaattt
    29401 agaatcttgc tcccttcaat aaaaatatca ggatatggtt ttcccctttt taataaagat
    29461 ggatttacta tacaatcaaa ttcatctttt attcccaaag attctaatat cctaggtgca
    29521 ttcttactag cagatgctat tcccaatttc acattatttt ttttaagttc ttttataagc
    29581 tcatttatcc ctggtaatat atctttatat gttaatgagt ttattaactt tatataaatg
    29641 ttattttttt cttcacacac ctttaacttt tcctctttag tataactatc tactttatta
    29701 gcagataaca atattttatc taatgattct tccctgctta tgccttttaa tttttcatta
    29761 aaagtttcat ctatttctat atctagcgat tttgctatct catgccaagc ttcaaaatga
    29821 tacttagcag tatctgttat aactccatca agatcaaata agactgcttt catattaatt
    29881 actcccttta aaacaattat taacttctat tatgttcttt gataatttta tttctttatt
    29941 atcatgtaat atggttatat ttctaccttt gattaactta taataagatt tatattcttc
    30001 tatatttaca gaaactaaat tatctctaaa taatatatta aatttaatat ttttccagtg
    30061 tttaggtatc cttggttcaa aacttaattt atcatttttt attttcattc ctgcaaatcc
    30121 atatactaaa ctcatccatg ttccacccat attagcagca tgtattccat cacttgtatt
    30181 tttttgtagg ttattaatat ccattaatgc tgtgtccata aaatagttat aagctttttc
    30241 atgatttcca atttcacttg ccatcattcc aaatattgac cttgacaaag atgaatcatg
    30301 agtagttatt ttttcatagt attcaaaatc tctcttcttt tgttctaagc taaactcatc
    30361 agggaaaagc aacgctgaaa gtacagtatc tgcttgttta tttacttgat atctatatat
    30421 tgttaatgga tgataatgga gaagtaatgg atatttttct tttggtgtat ttttaaaatc
    30481 ccatactgat ttttctaaaa atgaatcgtc ttgtttgctt attttcttct cttcatcata
    30541 tggtaaatac atattttgag cggcatttat ccagtttttc acttcatatt cttgtaattt
    30601 aacttttcta aatacttcat ttgaaaataa attatgctta aatttatcta ccatctgtac
    30661 tgcatatatt aaattatact tagccattaa attagtgtag taattattgt ttactattgc
    30721 tgtatattca tctggtcctg ttactccatt tatacagaat ttattatttt ttgattctat
    30781 ataatctcca aattcaaccc aaaatcttgc tgtttctata agaatttcta agccattttg
    30841 catcataaat tcttcatcat tagttgcttc aaaatacgtt ctaataccat aagctatatc
    30901 tgcatttata tgaaactgtg ctgttcctgt tggataatat gcactgcatt cattaccatt
    30961 tatagttctc catgaatata agcaaccttt atttattcca agttctctag ctctttctct
    31021 tgatgcatct aagattgagt atctataatc taataaagct ctagcaatgg atggttgagt
    31081 atatataaaa aatggtaata tatacatttc tgtatcccag aaataatgcc cctcatagcc
    31141 atcacctgta agaccttttg ctgatatatt agtctttcca tctcttcctg ctgattgata
    31201 aatatgaaat aaattggttt ttatccccag ttgaagagtt ttatcgcctt caatctctac
    31261 atcagaggtt gtccaaaact cattcatttt ctttctctga atatttttga ctttatcaaa
    31321 tccaatatta catgcttctc ttaaaaccaa ttctaatgat tttaaatgtg cttctgattc
    31381 taaacatttt aacgactcca gacattctaa tgattttaaa cgttcttcat ttagatttaa
    31441 atcagaatat actctactat agcctacaac aatttctaaa tttaaccttt catctttttt
    31501 ggcatagact tctatttctc gctcaataca ttcatctgaa acattatagg tctcatttgt
    31561 aatatttcca caagtaatat atgaatcaaa cccacaccat aaccctaatc tacttctttc
    31621 tgtaactaaa ttcataaaat acttattgtt atattttttt acattaattt taaattgctt
    31681 cttatttata gatgccactc ttgggtcatc tactttttca caattaaact tctccatact
    31741 attttccatt ttatttttaa attttatttt tccattaaaa ttcaatggtg ttattgtata
    31801 tgaaatcatc attaattctt ctatatcttg tgatactaat ctttctatag atactttaac
    31861 ttttctacca caagagtttt cccatataaa tgaacgttta agaatccctt catttaaatc
    31921 tagtatccta gaatgttcac ttgtaatacc ttttgataaa tcaaaataat ctccttcaat
    31981 agagaaattt actatatgag cattaggtaa cttacagata gtctgatttt gttttgcata
    32041 gccataagcg ctttctccat atgtaattgg agattcttca taaaatccat ttacatatgt
    32101 acccatatta tctacatact cattattaaa atcagactct tcaatattcc ctctaactcc
    32161 taaatgtcca ttagctagtg taaacagcgt ttcattggat tttattgtat ccttactaaa
    32221 cccttgctct ataagttggt ccttagtagt aaatattaaa ctcataataa cactcctttc
    32281 ttaaaatgaa ttatgcatga gtttccatgt cattaaataa aatttctcca tctgtttcta
    32341 ttactttttt ataccaaaaa gcactttgtt ttttagttct tttaaatgag cctcttccat
    32401 agtcatcaga atcaacatga attatgccat atcgttttgt catctttcct tcacttacac
    32461 tggttacatc tatacatccc cacataagat atcctataag ctctattcca tcttcattta
    32521 ctccaagaat catattttta atatgttctt tcatgtaatc tatacgataa tcatcaatta
    32581 tttttccatc ttcattcaca atatcttgag tacccagtcc attttctact ataaataaag
    32641 gtaactgata cctttcatat aactgattca tagttattct taaccctaca gggtcgtttt
    32701 gccatacact atttgatttt ggaagatatg gattttttat ggtttctggt ccgtttgcct
    32761 ttgatttttc taaaccttca gctctagctg aactacaagc actagaataa tagctaaaag
    32821 atacaaaatc tacagtatta tccttaagtg ttgataaatc ttcctctttt attattaatt
    32881 ttatttgatt tgattttaaa taataattca taaagcttgg atacacaccc ctaacttgaa
    32941 catcagaaaa aaacaaatta tacctttcat gctctaaagt tgcaaataca tcttctggtt
    33001 tacatgtgta tggatatgtt tttccagcag ctaacataca cccaaactta aattctttat
    33061 ttatatacct tcctatttct atagtttttg cattagctaa taattgatgg tgtgctgctt
    33121 gatatttttt ctgaagttga ttttcatcag ctataaaagt taacccagca cctatatatg
    33181 gaatatgcat tatcatattc atttcgttaa aaggaatcca gtatttgaca tcatctttat
    33241 atcttttcat caaaatttca gcatatttta catataaatt aaccactctt ctatcagccc
    33301 acccagaata tttttcaact agagaataag gtatatcaaa gtggcttatc gttattaatg
    33361 gctctatatt atattttttt aattctttta ttatattatc ataaagttgt aatccctttt
    33421 catttggttc actttcttct cctgttggga atattcttga ccaagatata gaaaatctaa
    33481 aacatttaaa acctatttct gctaacattg ctatatcttc tttgtaattt tcacaaaatt
    33541 taatccctat atgacttgga tagtattgat tattttcatt taaaatatta tcctcatcta
    33601 aacatacatc ttttaatctc ttatcattta atgggagttt atcaaactga gataaacctt
    33661 ttcctccatc taaataaaat ccttcacttt gattggctga aattgcccct ccccataaaa
    33721 aatctttccc taatttcatg catctacctc tttcacaata taaagtttta taatttctat
    33781 ctcatattta gttataagat ttttttcata ctataccttt taaataattc aatttaaaaa
    33841 tccaatgata ttttttactc actataatta atattgtcta ttttcaatac tttcatatta
    33901 aatatcaata ttagatatct atgtatataa ttatatatat agcaaataca tttttatatc
    33961 ctcattattg ttatcttatt ctcatatatt gctgtatatt tatatatata aaaggagtta
    34021 tttcgaaata gaggttgtat tgagaactaa aatttagact ttataaatga atggaatttt
    34081 cattgttgaa atactgaaaa tatatttgat ttcatataaa agaaagtgcc tcatgaacta
    34141 agttcatttt gagacactcc cttttttttc caagctatta tatctttttt taaattctaa
    34201 tggagagact ccaatttttt ctttaaatac tctgtaaaaa gtttttgtac ttccaaaacc
    34261 aacttcttca accaaatcta caatggatat attgttatta agtaatatcc actttgcttt
    34321 ttctaatcta tattcattta aaaaagttat aaatgttttt cctgtatttt ttttgaaaaa
    34381 tttagtaaaa taatatacac taaatcctac ttcataagac acatcttcta attttatagg
    34441 ttgtttataa tttttttcta catatttgaa tatattgttc aatctctcta atatctcttt
    34501 agagtttatt tcatattcta ctgtattttc ttcaatttct ctaggaatct gtctatataa
    34561 tataggaatt aaattataca ttttaccttt tattgcataa ctatatcctt ctattttgta
    34621 taggtcttca ttgtagatat ctatcaatat atcgaacact gactttctta tttttgctga
    34681 ccaatctcta ctacattttt ttatactact gaataaatta attaagcttt ctttttcctc
    34741 atttaataaa attaaatcat taaaaaaaga tatatcaaat tgaaatacta atctttcact
    34801 tcccggtgat ggcagtatat aatgaatatc tcctcctttt attatcacta tctcgccttt
    34861 ttttaattgc ataggcttgt catttataaa tagatttact acaccttctg ttataagtaa
    34921 tatttctatt tccttatgcc agtgtggtgt tgttaatact tctccttcat ttgatagact
    34981 acgaaaagga aattcatgta ttaatttagg catttctaaa tatattgcca tatatatcct
    35041 ctcctctcta gtgtctaaaa attaatttat actatttaaa agaatacaca ttttatttta
    35101 aaaacctcaa atttataatt tatacaatac ctacacttca taaaatatat ctaatatttc
    35161 tttttttgta tgtgcattta aaagtctttc acctatattt ttaccaccta aaatgtaagc
    35221 tattttagat attaatatca tatcttcatt gcctccagat tgtggcatag caaacataat
    35281 tattaccttt acaggtaagc catctatact tggccaatct acaccttttt tagatttacc
    35341 tattactaca gaggccttct ttacatattt tgtttttcca tgtggcatgg ccatttgata
    35401 acctatacta gtagagccta ttttttctct atgttttaat gcctttataa acttactttt
    35461 taaagttata cttcctgtat cgtataattt atctactaat tcctgtatta tgccttcttt
    35521 tgtggttgat ttcaaatcta aaataatgct ttcttctgtt aatatttctc taatttccat
    35581 ttgttttctc caatagatta ttcttaaata atattttaat atcctctttg ctcttgcaat
    35641 ctattatata gtttatatag ttcttttgcc tatataaatt tgatatttcc attaaaatat
    35701 tctttaaaaa actttgatta ctaatttcta aataaatatc aatcgttcta gtaacattat
    35761 tgacattcat tatcccttta tttaatttac tttgcttatg tatatatata tttaaatctt
    35821 ttcctaatga aatagattca tatttactcc ctaaattcac acctattaaa ctttccatct
    35881 gagctacatc aaaattaaaa tatatattat cttctaaata tattcctaat aagtttgtat
    35941 ctaagttcat acttttttta ggtttagaaa gtaaagcatt ttttatatta ttaatatcat
    36001 tttccattaa tagtactgat acaactatat gtaatttatc tttcaaatct aagtttacag
    36061 tagatattac aaagtctaca tcatctaaat ttcgcttact caactgatgc ataggaacta
    36121 tatcaactat agtccattca ggaaattctc tttttaatct ggctgcaagg agctgagatg
    36181 ttccatatcc tgtatgacaa actactatta ctcttttact actcatattt ctttctattg
    36241 ccgcttgaaa atatgtggca agatacgaga cttcatctat actaatattc ttcagattat
    36301 catactctgt cataatattt atagtcatca tacataatcc cagcacatct gaaaatcgct
    36361 cttgtatctc tcctaacaac gggtttttta tttggatatc atatcttaat ctgtttaaca
    36421 taggccttac atgagatgct agacttttct gtagtaagtc atcatttttg agatttatat
    36481 ttaaaaatcg tgacatattc tctatcatta tagatgaaac tttttcactt tcacttaatc
    36541 tatctgattc atcattgtaa ctactattat tttcgctact aaatccagat gatactaaaa
    36601 aaatatatat gtacataatt tcatctttcg ttatttttac tctgtatcta ttttctattt
    36661 tatctataag tgtagtgaca tttttatata ctaactcgtc caaattatct acagaaattt
    36721 ctctttcttc ctgagattct atttgattac cttcttcgat tctctttaaa catataagta
    36781 tatgggtaat taaatttata tagtagggct gatttatagt atatcctaaa ttgtattcta
    36841 attctgtaat tatagtttct acaaatataa tagaatcaat atcaaataaa tttatcaatg
    36901 cactaaatgt agcagaatct aatcttgtta actcagatat atcatgttgt tcataatttt
    36961 catctaataa atcatccatc attttggcaa tcgatttcct tatatccact tctttaccta
    37021 taatcttagt tccttctaaa gttttattaa gttttaaatt gtattctttt atccatgcct
    37081 ctatatattt aaaatcattt actatagaag ttttacttac ataatattta tctgctaact
    37141 gatttattga agttatatta tttgaattta gtaataaata ctttaaaatc tccattcttc
    37201 tctgctctac tgataaagat ttattttcct tcgattgtat atttaaacta ttaattaagt
    37261 ctatgttctt ctttgctaaa tcttctatta atattccata gcctcttttt atatcaattt
    37321 ttatattaaa agtttctaaa tatttctcaa tcatcttcaa atctttttgt aaagttttat
    37381 cagatacatt taatttatct ttaaagtatt ttataggtct atatttattt tcgtttagta
    37441 tcaatttaag aaattcaatt tgcctcgaca caagcatact tagccctccc tacctatgtt
    37501 tatatatttc tatgtttata ttaagtacta ttttaacata tttactaact atgtaaaatt
    37561 actaagatac ttctgtatca taaaagaatt tatgatacac cttactaaat tgatataata
    37621 gataatataa tttcaaaata aaaattaagg atatttagaa gtagttaact gattctaaat
    37681 atccttatta ttattctata tacttgttgt tattccatta attattttta ctattattta
    37741 taatttaagc accaagtaat tcaacttcac tattttcact ccttatagta ggctttgtta
    37801 cccctataat caatgctgtt actactgtac caatcaatat acaagctata tatgcaactg
    37861 gtgcactaca tgcaaatggt attacaaata ttcccccatg tggtgccatt aaagttatat
    37921 ctaaactcat acatattgcc ccacctatac aagaaccaat tatatttgct ggaatcattc
    37981 ttaatgggtc tgcagctgca aacggtattg cgccttctgt tatgaaagat gcccccatta
    38041 tagcagttgg tagagatgac tttctttctt ctttagtaaa cttatttttg aatattaaca
    38101 tagccaatga tatcgctaat ggaggtacca ttcctcctac cattaatgct gctgatggtt
    38161 caaatatatt acttgaaaac atagccaatc caaaagctga agctgtttta ttaataggtc
    38221 ctcccatatc tgatgccatc atccctgcta atattgcacc taataatact ttattagttc
    38281 cagacatact ttgtaaccaa gattgtaaag cttcatttaa ccatgatgct ggcgtattaa
    38341 ctacaaagaa cattatagct ccactcgtaa atgcaatcaa aagtggacat atcaatacag
    38401 catttactcc ttttaataca tctggtaaat tttctgttat cttcattact cctaaagcta
    38461 tatatcctgt tgcaaaacca gctaccatac cacctataaa acctgaacct ccaataactg
    38521 ccataaaacc agctatcata gcaggagcaa atccttgtct tcctgctatt gatttagcta
    38581 taaaaccagc aagtatcggt ataaataact taaatgctga atcgcctcca agttgtccaa
    38641 agaatccagc tatagggttg tatgtttcat gagttgggtc tgatgcaaat ataccaaaca
    38701 taaaagctat ggctatacca attccaccta tcactacaaa tggaatcatt tcagatacac
    38761 cactcattaa gtgcttataa acaccctttt tctctgaccc ttgaagcatt tcttttgaat
    38821 cttctaaagt ctttccacct ggtttaattt ctttagccat tccattttca gcgtttataa
    38881 ttaaattttt agctaatgaa acattcttag catctactac tttcttttgt agcactttct
    38941 ttcctataaa tctactcagt tgaacttctt tatcacaagc tattattacg tattcagctc
    39001 tttttatatc ttctttagtc aacttatttt ctacacctat agctccatga gtttctattt
    39061 taacttcata tccaagttct tgacctgctt cctctaatgc tttttttgcc ataaaagtat
    39121 gagctatacc tgttggacat cctgttatcc caactataag acctttattt tgattattac
    39181 taaaatctcc tgattctgca gctttattat taaatatact taatatttcg tatggagttt
    39241 ctgcacttag tagatgctta agaaaatttt cttccattag atatgtagct agtttagata
    39301 taatcttaag atgttcatct gaacctgatt ctggaactgc tatcataaaa aatgacctaa
    39361 gtttttgacc atcattatct gtaatctctc tatctgttct taaatacatc attacaggtt
    39421 gatttactac atttgactta gcatgaggta tagctattaa attacccata ttagttgatg
    39481 attctttttc gcgtttatta atatcagcta taaacttttc tttattattt atataatttc
    39541 catggtacaa atgatttgcc atataattaa ttacatcttt ttttgtttgt aattgtttat
    39601 ctagtacaat acaatcgctt cgtagtattt tacctatcat aaattctccc ctccttttaa
    39661 tttgttacct cataattaat ttttattttt ataaaagcac tttaacttat ctatagcatt
    39721 gaatagattc attttaaact ctataacttc tcttgcagat tttatacatg gtggatatat
    39781 agtatctggc tcatataagc ttgggttttg ttctaatagt tctctgcact tcttataata
    39841 tgccgactta acatcactcg aaatatttat tttacaaact cctagtttaa ctgcttgtgc
    39901 tatttcttca tctggatttg atgaaccacc atgaagaact aaaggtatat ctactacttc
    39961 tcttatttct tttaataaat ctagtttaag ctctggttta aaaccttttg gatatatacc
    40021 atgtgctgta ccaattgcaa tagctagtgt gtctactcct gttttttcaa caaaatcctt
    40081 agctaatttt gaatctgtat atattatttc atcagtacca ccttcacttg atgttcctgt
    40141 tgttcctatt gttccaagtt ctccttctac ggacacattt aatccttttg ctatttctac
    40201 tatttcccta gatatagcta cattctcttc atatggtaaa tgtgagccat caatcattac
    40261 tgatgtaaat ccacatctta ttgcccttaa tatttgttct ttattttccc catgatctaa
    40321 gtgtattacc ataggtactt tactcttatt tgcttcttct atgcaactag ctacaaagct
    40381 atctcctaga aaacttagct catttgggtg tattgcaagt attactggtg catttttttc
    40441 gttagcactc tgtactactg ctttaagtat ttgcccactt cctatattaa atgctggtac
    40501 agcaaattga ttttcttgtg ccacttttag catttccttc atatttatta acatattgta
    40561 cctcccgata ttttaaattt taggttgaat ttgcttcttg ttaattttgt tgttgtttat
    40621 atttaaataa tatcagtgta atgaagatca gaaaataaca atcgttttcc ttagtgaaaa
    40681 ggaactttta ttatacactc taagttcctt ttcactaaat aatttttaga agtctgcatc
    40741 atagttaatt atatttctca tatacatctt ctctttttaa gtgttttatg ctttagcctt
    40801 cctagatttt ataataaaca attaagatac attaactata aatttaaaga tactcatgca
    40861 atagttaatg ctatattaga gtcaaaaaaa ttaaaaagat attagctatt tggccaatac
    40921 cttttcttgg attccaatag tatatatgat ttatagaaat catactcacg attatctaat
    40981 gttttacttc cataaacttt acataaaaca ccaaagtgac accctccaaa ttcatctcta
    41041 gctaataaag tgatttggtt ttctaagcag caatttataa attagccttt gttatcttat
    41101 gtaaatcttt ttctatctaa tttaaaaatt attcctaatg ctatatagca tataccaatt
    41161 actaatacac cataattcat agatactcct atcttaaatt cttgcatatt agttacaata
    41221 gaaattaaaa taggtttatt acaaacacca taaagcaagg ctgacattgt atattatcca
    41281 attgtatgaa tgaatactaa tatagctcct tttaattaaa tgatgctaca taaatcatat
    41341 ttatcccaat ttttaatcta acctatttat atgtatattc ctttttaata aaaaaagata
    41401 ttgactaaat agtcaatatc ttttagttga tttaaatacc cacaatttta atctaatttt
    41461 taagtgtttc cttaacataa tcttataaat attaaaattt atctacctat aaaatttctc
    41521 cattaaattt tattacattt tatcttaaaa caattataat ctgaccttaa tcacatgtta
    41581 tattatataa atatttttaa caaacttaat gattaaataa ctatatttta ttatccatct
    41641 tataaatgtt tataaatttt ccactattct tcttcaataa attcaaacaa attttctact
    41701 gtagtatcga atactttagc gatatccaca gctaacttca aagaaggatt atatttccca
    41761 ttttctaagt gtacaatagt ctcacgtctt accccaacca atttagcaag ttcattttgc
    41821 gtcataagga gtttttctct gtattcttta atttttgttt tcatcaatat tgccacttag
    41881 ttacatatcc cccttttatc aaagtatata tacaacacta atttagataa agactgtata
    41941 aataaaactg caatgaggat tatacctgta tctaggtttg atatatttat attacttgta
    42001 taaggtgttg caaagaaaat agatattatt actatagaat atatcattat ttttattgca
    42061 atcttattaa cctttgatag tataaattca gatgattcat caactaaatc attatatttt
    42121 ttattataaa ttgaataaca tccaaaaact attaaccaac tcaaccctgg aagagaagca
    42181 gtgcttggac ctttaaaaat atacttagtg gataatacag ctataaaaaa ataagttatt
    42241 acaagcaact ccaggataag ctttgatctt acaccagttt ctgatttaga catataaaat
    42301 cactccttaa aaattatatt ctctatactc tttaattttc gtttttatca atattatcac
    42361 ctagttgcat attccctttc tatcaaaatg tatatacaaa attaatttag ataaagattg
    42421 tataaataaa atagctaaga gaattacacc tatatccaaa tttgatatat ccaaacctct
    42481 tgtataattt gctgaaaaga aaacagctat tagcaaaata gaatataaca ttatttttga
    42541 tgcaatatga tcaaccttta ataacataag ttttgacgcc tcgtcaacta aatcattata
    42601 ttttttatta tacatgaagt cacatgccca aaaagcgaat aaaattataa aagtaataca
    42661 aagatttcca tatttaaaaa taaacttttt taataatgag tctataaaaa aataggatat
    42721 cataagtaac tctgaaacaa gatttgtttt aacaccaagc tctgatttag acatataaaa
    42781 tcacccctta aaagttatat atttataaca ccttatgtta taaatatata acttttccaa
    42841 ataaattgca atagaatatt gaaaatattc aataatatct tcataaatat attttataaa
    42901 aggttttata ataaacatta caatacagta ctaaattagt ttatttcatc tatcatctca
    42961 atcatatcat ttactaacta ttattctaat gagcatataa cattgaaatt tataattata
    43021 caacctaaat gcctcccaat gtaccctcaa aaacatcatt tcctgtaact gaagtataat
    43081 tggtatattt tatcactgaa ataaaaaaac actagtcaat aaagactagt attttcaagt
    43141 attttatcat tcttttttta attattggag acgaatcatt agaataatgc ccataattta
    43201 tctatatgca aattctaaat ttaaaataaa gtttcagata aatttactat tctatgctat
    43261 gatacaacag aaaaaactga aatctatgag tggtagcttc atataatcct agttcctaca
    43321 gtaatcaaaa ctgaggttta ttctatatgt aactaatgat aaattttatt ataggagtta
    43381 ttagctctat ttgtgttact ataagtagta attatatata tgataatttt ttaacacttt
    43441 tctgttggct atatagaaag tggtttagaa tttaaattta aataaaattt aaattcacca
    43501 aaaagaaatc tatattagaa accactactc tattccacca gattgtaatt ttttgttctt
    43561 tatatcacaa tgctatacca tattaaattg aaaaataaat tttaaattta tttgttacaa
    43621 ttcattaatt caaaaaaata ttttgagtca tgagttttgt aaagatttag ccatgaattt
    43681 aatgcttctg attcaaaaac atctaaaagt ttcaatactt gaaacgtaaa ttccaacgga
    43741 gcaattcctg aagccgtaac taaattttca tcactaacta cagattccgt tttataatat
    43801 ttttctccag agtaatttgg tattaccatt ttaagaaact ctaagtcatt acttgtatga
    43861 tttctaaaat ctaatagccc ttttttagct aggcctaatg tagctccaca tatggctgca
    43921 actatagaat cttgtattaa agcttcttta actttttcta atattatctc atgattcatg
    43981 gataaccaag tatttccccc tggcaaaatt aataaatcct tactttccat attaaactca
    44041 tcaactgtaa tttcaggtaa aacttttaac ccacccattg ttataatagg atttttatca
    44101 attcctacag taattacttt tagtggtttt aatccttttt taaaatatct tcctgtattt
    44161 aattcagcag ttaaatatgc cacttcccaa tctgacatag tatcaaatac atacatatat
    44221 acttttctat ttttcatctt ttttactcct tatgtaatta atttatatat ctaattataa
    44281 aataacttaa ctgacatatt cagtcagtta agctattaaa tttgataata atcaattaat
    44341 tctagtaaat cttcaattat tttcttccta aggcttagtg gttcaattac ttgtattgtc
    44401 tttctataag gtaaaagtat atttggtatg tacttgcata ttacttcctc ttcaagcata
    44461 aatattattt tattaggtgt tctttctttc aagtagtgcc ctaaaaacca atgttgactt
    44521 aaactatcta aagaatctat actcccttct acaactaatt gaattatatt ttgttcatct
    44581 tttatttcag gtataattcc ttttataaaa aattcacttg ctgaaaattc tgtcggtctg
    44641 ttaaatgtat ttgatgtttc tatgatttca ctaatcctat ccactctgaa actacgtacc
    44701 tcatttctca tattacaaaa tgcaactacg taccaattat tattccagtg aacaattcca
    44761 tatggattta taattctata ttttgatttt gctttacgac ctgcatggta gttgatttct
    44821 acagatagct cctttaatat actattctcc aattttttaa gtactgattc tatagacact
    44881 ttcccaattg gttttattac ttcaagggct cttaagtggt ttgttaaaat atcttcttgt
    44941 ttttgatttg aatacatttt taactttaat atcgatttat ctaatgcttc gtctaagaag
    45001 tatccttctt ttttagcaaa tagagctgca tgaagtagag atttttgctc ttctatatca
    45061 aaaaaaagag gtgctttagt aaaattattt aaaaggctat acccaccatt atgacctgta
    45121 tctgaaatta taggtactcc actcgcagaa agtacatcaa tatatcggta cactgttctt
    45181 atatttattt ctaatttttc agctatctgt tttgctgtaa tttttttgtc agagtttaat
    45241 atccacagta tagacatcat attgtcaatt ttagccatgt agtttcacct ctttctattt
    45301 gttatataaa gttattatat aacttctgat cgatttttta caaaaaaaca ttagtaaact
    45361 gctaataatt tatactaaga tttatttcaa atatttatag agagtctttt tctatagtta
    45421 agttatccat taaattccat agtagtgtat ctacttcaat tgatatattt tcaactacac
    45481 ttctcgttat atatcaattt tcttttttaa agattttaca actgatattt agttcaagat
    45541 ttatatatat tctttttcaa aaacctttca gtctcaaatt aaaacttttt tgttttaatt
    45601 tcttcaagat atagaaaaag tcttatccta atttatttaa gctaaaattc ttatatttta
    45661 aatttttctt taaaactaaa atatttatta tcatcttcag caacaatata aatctactaa
    45721 ttaaatacct aatatttatt catatttttc taaacataat tactaaaatt gaattaatga
    45781 tatcaattcc attaatctct tttaataaaa aattctcttt tctatatgaa aattatttca
    45841 aaactttaaa gttaaataca aaaatgacta taaataggtt tttatttaca caaatctatc
    45901 tatagtcact atagtttatc caataaaata ataaaataaa tatgtacaca ataaatgtta
    45961 tttacgtctt aagtatttat ttgttagatt tttaattaaa aaaaacaagc taatgaacat
    46021 taatgctaaa tctagcatta tattgctttg atctcctgtt tgtacatttt gacctacatt
    46081 actatctaaa ctttcatctg tatctatatt tatatctggt tttccattcc catctgtatc
    46141 tatatttata tccggctttc catctccatc tatatctata tttacatccg gctttccatc
    46201 tccatctata tctatattta catctggttt tccatctcca tctgtatcta tatttatatc
    46261 tggttttcca tctccatctg tgtctatatt tatattcggt tttccatccc catctgtatc
    46321 tatatctata tctatatctg gtttgctatt tcctccatta tctgggtctg gtttactatt
    46381 tccaccgtta tctggatccg gtttgttatt tcctccatta tctggatctg gtttaccatt
    46441 gccaccgtta tctggatctg gtttgctatt tcctccatta cctgaatccg gtttgctatt
    46501 tcctccatta cctgaatctg gtttgctatt tcctccatta cctgaatctg gtttgctatt
    46561 tcctccatta tctggatccg gcttgctatt tcctccatta tctggatctg gcttatttag
    46621 atttggatta cctgtatcat aacctcctga gccagtatct acatttttat caggcttcca
    46681 ataatcatct actccatcat tatctgtatc tattgctgga cgattataat ctgtatcagg
    46741 gtttccatct ccattggtat ctatattttt atcaggtttc caaataccat ctccatttcc
    46801 accttctgta ctaggcttcc attcacctgt attatcagta tctatgttaa tatctggctt
    46861 ccaatcacca ctcgtatcta tattgatatc tggccttcca tcaccatcag tgtctatatt
    46921 tatatccggt attccatccc catctacatc tacatttgtc acatctaact ttaacttctt
    46981 ttcatcctca ctaccttcaa tgtaatttat atcatctcca atatatttta cagtaatagt
    47041 ttgttcagta gtaaaaatat gatgattgct atctaaaggt ctccattcaa tagatgcagt
    47101 actataatta tgactctcac tacccttatt atgagttttt aattctactg gatctttata
    47161 ttcctttcca ttaatataga attgtacgta acctcttgga gaggcacaat ctggtccttc
    47221 tttcttatct ggtgaaatag taacatttaa tgtaaacgta tctgttggtt taaaaacatc
    47281 agttttagct ttagactctt ctacaactaa tgaagtttct gtatcagcag gaataatttc
    47341 tgccttataa tatgctctat gcccccaata agcttcatta aaaggatata catttgaaaa
    47401 ctgtgttgat gtaatgctta attgatattt tccagcatca acttgctttg taaggtcatc
    47461 acttcctaaa tttgtatctg gttccattgt ttcctcattt aaacgttgag aactaatagt
    47521 cataaatttt ttatctgtga gtttttcacc tttaggagta attattggaa tacccccatc
    47581 ttttaatatc caatctaaaa atgatttttc tacatcttct tcaaatggta atccatcata
    47641 tcttttaata atcaaatatg gtggagtatc atcaatagta aattcttcat attgctttaa
    47701 aaattgagaa cctgaaccta tatctttaac ataaaaatca tctggtttta tatttcctcc
    47761 agtaatttta tctataacaa ctaaattaac agatacttca gatcccttaa attgttcaga
    47821 aaaccataag taaagaaccc cttgatcatc tgtataacta ggtgctccat aaggataaat
    47881 ttttccatca attagcattt taaaatcatc aactaatgta gtactaactt tatcttgtcc
    47941 taaatacttt aaagatattt tattcataaa aacttttgtt ttcttttcta aatcaccgta
    48001 tgcaactgct ccacctaaac ttttgaatgt tgttgctttt aatgaaccac ctgttactat
    48061 aacatctccc gttgtagcac ttaggtcaaa aatccaatct cccactcctg tcatataagg
    48121 taaaatcgtt cctccagtta caagtatacg atatcctcta tttgttcctg gattaacaca
    48181 tccatcccca aatgcactac catgtgcacc tccatatgaa taggaaagtc caccattaat
    48241 tgtaatattc ccactataac cagaacctgt gctaggatct actactgagg gacttaatgc
    48301 attctttgtt gttgtatagt atcctccacc tattccagct ccatggtatg cacctttagc
    48361 agttatagta ccaccattga ttgtgatatc tccaccacta ccattaccgc ctccacctat
    48421 tccagctcca tatcctgtat tacgactcga ttcttgacct atagcagtta tagcaccacc
    48481 attaataata atatttcctc cactttcagt atttccaccg ccaatacctg cagaataata
    48541 atttccaaca acttttaaac ttcctctatt attactatct agtagtgtaa ggcgatctcc
    48601 tttttttaac ttgtctccat tttctagagt tacatctcta ggaatttttc caccctcagg
    48661 aactataaac tcgccttcaa tatctctatt tacaacagaa tcatcaataa ttagagtagt
    48721 accttttcct gctctcatac ctggataata tacatcacct gactctagct tgttttcact
    48781 accatcttgt aatataacat gtgcatatgc accaccatct ttatctggtg ttaaagtaaa
    48841 tgggctatta gatgcagttt taatatttac tccatcaaat attaattcag cttttacatc
    48901 tttaggtata acaatcctat gtgttgttgg agtatttgga ttattatttt gaattgtcat
    48961 ttgttttcca ctaagaatag ttaaaacgcc acttgcaaat ttaaaatcga catttatttc
    49021 tccaccacta attttaaagt ctcctacaga tgtaggatct tctgcagtgg cttcttccac
    49081 aactaatgca ttcatatctc cttctgcatt tcctgcatta gaatcttctt ctgcatttcc
    49141 tgcattagca tctccttctg catttcctgc attagcatct tcttctgcat ttcctgcatt
    49201 agcatctcct cctgcatttc ctgcattagc atctccttct ggatttcctg cattagtatc
    49261 tcctcctgca tttcctgcat tagcatcccc tcctgcattt cctgcattag catcccctcc
    49321 tgcatttcct gcattagcat ccccttctgc atttcctgca ttagcatccc cttctgcatt
    49381 tcctgcatta gcatcccctc ctgcatttcc tgcattagta tctccttctg catttcctgc
    49441 attagtactt tcttctatag tatctgcgaa agtatttgac gcattaaatg atgctactga
    49501 tccaagtccc attgagatac ttaaaatcat tgatagtgct ttctttttac atttttgttg
    49561 cataaattcc ccctcccctt aacaattttt aatatacact gtatacatcc ttatatacac
    49621 tctaataatt cttaatatac actgtataca tccttatata cactctaata atttttaata
    49681 tatacttaaa acttatattt gtcaatgaat atacagtttt tttcaacctt atatattata
    49741 agtattcatt atttgctaaa taactacaat aaaaatttta attatataaa atttatttaa
    49801 tcttttatat aataatacaa ggggtaatta attgaaaaaa ctagtaaaaa tattaataaa
    49861 tattatttgt attattacgc taactttctc tagcctttcg atttacataa aattatctga
    49921 atataaaaag ctgatgaagt atatacggaa ctcagagaaa acactatcaa taatagcaaa
    49981 catcaagaac tttatgataa aaataatgat tatagattct ggcttaaaat aaacaacgta
    50041 aatatagact atcctgtagt acaaggatat aacaatgatt tctacctaac tcatgatttt
    50101 tataaaaatt atcttccttt aggtagtatt ttcatggatt atagaaataa ttttgaaaat
    50161 gataagagtt taatagtcta tggtcattat atgaaaaata aaactatgtt tggtcagcta
    50221 gaaaactata ctgatgaagt atttttcaaa gaaaataatt tagtagaaat aaattataaa
    50281 gtacaaactt atacttatga aattttttca gtatatactg ctgacttaat taacagagat
    50341 tatttaagta tacactttaa taataatgat gagtttaaat attctttgaa ttatataacc
    50401 taaaaatcag tacacaaatc tgatataaag gtagatattt cagataaaat tataactttg
    50461 tatacttgta aatatgaatt taaagatgct cttactatag ttcatgctaa attaatttca
    50521 aaaaagagac ctataagtag gtttgtgtaa atgcagactt atctacagtc ttttttgaac
    50581 gtaaaattgg taagtataga ataatattca tattaaactt ttgtaggcaa agaaatgaaa
    50641 aatcaaagag aaataattat tacagattta gattaccaag ctgaattaag agagtgtaaa
    50701 actatggagg atgtagttgg tgaagataat atataatagt ttaacttata aattatcttg
    50761 gtgatattaa tgaagtttca aagtatatat tatttattga tataaataaa attaaatctg
    50821 tttctaatag atgtaccttt gtttggagac aaactattga agaaatataa ctaaaatgtt
    50881 gaagtagcta cctgacttta ttttgaaatg cgaacaagat tttggcatta agataattta
    50941 gaaagaatca ctggtacaca aggatgtgaa cttagaatga atagaagtat tcaggcataa
    51001 ggagctttca cccaaataaa acatagtatg aactttaaaa gaattctaat caaaggcaat
    51061 caaaatattc tttcagagtt tatacctgta gtgttagcct atagtatttc taagtttcac
    51121 aaataagata atcattaata aatgtagtat gtatattcat cctctcaaaa atatttttta
    51181 atcttacact ttgtaaatta ttctatttta aaattgtatt tttatacctt tatttcttat
    51241 gaccgttttc aagctcaaat tttgcataaa tataaattaa tgaaaagcaa gtaaaaaaaa
    51301 actgtcgcac agcaatttaa aattactcat tcgacaatcc ttttttcgtg gatttaaata
    51361 gtggagaaga atcacaagaa taatacccac gattaaacct ttatacaata cattttatat
    51421 gactttacca tcactaatag ttattataca atcagccatc ttagctattt catcattatg
    51481 agttatcata accaacgtct gcttgtattc atttacacaa tactttagta aattcatcac
    51541 ctcattagtt gttttactgt ctaaatttcc agtaggttca tctgcaaaaa ttattgatgg
    51601 cttattggca agtgatcttg ctattgcaac tctttgttgt tgaccacctg ataattcatt
    51661 aggaaattta ttaatctgac tttctaatcc taacttagtt attaaatcat taatataatt
    51721 attatctatc tttcctttag caattaaaat aggaagaact atattatcat aaacattgat
    51781 aacaggtatt agattatagt tttggaatat aaacccaaat tctttacttc taagattaga
    51841 tagcttttca tcacttaaag tatataaatc taaattatcc ataaatacat tacctaaagt
    51901 tggtttatca agccctgcca tacagtgaag taatgtgctt tttccagaac cactttgtcc
    51961 aattattgca ctaaatttat ggggttctat ctctaaagta attccatcaa cagcctttat
    52021 ttcattttct ccttttccat atattttttt aatatctata acttttaaca tcttcataca
    52081 tttacctccc aaattattca ttctctttta taccttcaat aatattcata ctttcaaaat
    52141 ctctattact tacaactact gctattaaac atacaaatac agatatagca aagaatagta
    52201 aagattcctt aattgggaat gtaaaagaca taaaattctc tattccatag tttatatacg
    52261 catcattatt ccaatttgta aatttaacta gatttataaa tgatggaatc atagctatta
    52321 taaagctaaa aactgcatat gctaatgctt catatataag catttttttc atatttttaa
    52381 gactaacacc taatgctcta agcgttgaaa tttctttttt tctaattagt agatttgttc
    52441 ttatagtaca gaaaatatta attgatgcta ttattaaaac tagtgcaatt attgctattt
    52501 gtgatttaat gtaactttta tatatacttt ctgacatttc atgataactt aatctactat
    52561 taaatagaga ccctggtata cttcttgaaa ttttctgtat ctcattatta acaatctcat
    52621 catatggatt ttctaaattt atgcctaatt gagtgtattt ttgttctcct gttatgtttt
    52681 tagagtgaat attacttgtt ataatttcta gatctggcat aaaatcgcca tctcccatag
    52741 caatccattc atcctttaaa attacagcca ctcttacctt catctcttta taaacaattg
    52801 tattattttc ttcaacagga atttttacag ttattatgtc tcctatattt aattctttga
    52861 atataggctt tatagtatga tcatcaacta tatcataaaa ataattatat actgctacat
    52921 ttggatatcc atcagtgtcc tttttaatat catctaagct accttcttca acaaagtcat
    52981 ttaacttttc atctatataa tcatcatcat aaagctttaa aactaattta tcttccatta
    53041 tctctttttt ttctactcca ttataggttg taaattcttt gtttatatcc ttattattaa
    53101 aagtaaacat tccatttgtt atttgaattt tatgtatact tttaacaccc ttaatttttt
    53161 ctatattctc tacatcacta tccgtgtatt ttgatatttg cgggtctgca tttactccaa
    53221 attgcattgt tatattatat ctattttgca tatcttcaat tttactgttg acctcttctt
    53281 gcatacttga gaatgtagta atgaatatat atcctcctaa agcaatagac aacatagata
    53341 tagcagctct aaccttattt cttccaatat ttttaagtcc cataaatcca taaaatccaa
    53401 atattttgct tactagtctg tctattccaa gtctacttct gtgattttta attttgtctg
    53461 tatttattat tgattcaata ggtgatatct tgccgatttt aaaagttggt ataatacaag
    53521 atataaaaac agaaattatt ccaaccaata tagcttttat aactatttca ttatttatat
    53581 ataatttagg gttaagacca gcttcactgt ataaagcttc gttataatta ctaattccta
    53641 tatatgaata tgtaacccca gctactaacc caagtagtat acctattatt gttataatta
    53701 atgcttgata gattatcata caccttacgt ttcttttctt tgcacccatc agtctaaaca
    53761 gtcccatttc ttttactgat tgatttagtg taatattgaa aatattaaat atgaaaatta
    53821 tagatactgt agttattaaa acatttcttt taaaaacatt cggatcactc ttatcatctt
    53881 ctacataacg aagttccctt acaagaggta catttggtag aaagcttttt tgtcctaact
    53941 tatatttttt cataagataa tcacattcac cttcaatatt tctccatcca cttttaaaac
    54001 ttaatatact attataacta atcgcatcat taggtataat gttgtttgtt tcatcattgc
    54061 cataagttac tgcttcgtat tgccaaacca catcatagta cccttttggt ttttccataa
    54121 ctcctactat tttaaattct ttatttatag aaaataattg attacttccg ttagtatctt
    54181 tatagttctt ttttattgtt aaatttaatg tagatcctac tccttcactt aaattcattg
    54241 cttctagtgc ttttttctct aatactattt cattgttgtt tttaggtagt ctaccttcta
    54301 ttattttgtt gtatctactt tttagatact cttcgtttga tgaagttaat ataaatgagt
    54361 ttcctttttt atctgaaata cgtcctagat tttgtaccga attacccttt gatacttttt
    54421 catcagtttc taatttttta gcatcatcaa gtgtcaaatc actaaactct acatggtatc
    54481 cactgtaaag ttcatgtatt gctactacat tgttttcatt ttgtgaatct tttataacac
    54541 taggtccaaa tatcaacata acacctaaca taactcctat tattaaaagt aatgttctaa
    54601 gtttattttt ctttaaatat gctagtgaca tttttaagta aatccccata taatcctcct
    54661 attttactat aaattattta tccaactcat ctgttaagta gattggtgga tattttaaat
    54721 cttttaattc ttcattattt tttgaagctt actaccatct tcctcatgat aaacttgtag
    54781 tatcacctta tttacatgta gtgcataata atcaccacta taaaaatatt ggtattcata
    54841 acctgaataa tttttgtaaa atgatttata aagtacccat cctaattctc ttttgtatac
    54901 ttttcttttg taatacgctc tatatcatca cctgaaactc cttgatatgt catatattta
    54961 tctatatatt tttcagcttc attccatttt ctaaaatagt tgttgtatat aataaatcct
    55021 attatggcta taatgcttat aattaaagtt aataaaatag ctttgtattt tctcatagtg
    55081 aatcttccta cttcaattta aattctttaa taagttttaa ttctaaagac ttagttttat
    55141 catcaaattt cttaacttga atttttatat ctcccaaatt atcaacctct atatttcctt
    55201 cacctttatc aaatacttct tcaatttttc taaaaaattc tttctcactt atttctacat
    55261 gaatcaatga tctatatgtt ataaatggcc attatgaatt tatcaccttc tgacagcttt
    55321 tcattataag aaaaatcttt aacacattga attgataata tttccttcgt acccttcttg
    55381 tccttagtca ttgaataatg taaaacttgg ctagattgtt cttttgtata ctccaagttt
    55441 tccaaagaca aaacaaaact tccatctgat ttattttcag ctaccctaag accactattt
    55501 tctattgatt ctgatatttt ctttgacaag tcttcttgat actagaaatc tgttcaatgg
    55561 tatatatttt ttcatttgta tctgaaacct tatttgtgca tcctgttatt aaacctagcg
    55621 ttaagcttat agctataacc atagttattg taattttttt tatcttcata taatccactt
    55681 tatatattga aaactcgtta tttatcataa aaaaaagacg ctatcttttt ctattaaaag
    55741 ataacatccc tatatgttta gtatcaatat atgttcccca actggtaggt taatcgaccc
    55801 aactatcagt attttgattt aatggtatat aaatattcaa tataaatttg tcttttttat
    55861 cttcaaatga taaatctcct ctatattttt ttatagaaga tttgatgctt taaatcccta
    55921 ttccatgtac aagtttatct tttttatctg ttaataataa acttttattc tttctaattg
    55981 aatttatttt actattctca catttaatta cgaaatagga ctttgagata gttcctttta
    56041 tttgtatata tttatcaata cttttatcat gtatcttatc gcaagcttct attgcattat
    56101 ctaagtattt gcaaatatat tagatacatc tattggtttt ataaaattca ctttggaaaa
    56161 attgatatca caaatgaatt taatttttct tttttcactt acatcacttt ttttatttat
    56221 tattatatcc aatataacat tacctgtatt gtaaattgat ttaaattctt taatttaatc
    56281 ttttaaacta tttacatacc catcaatttt ccttttttat atttgagatt ttctatacaa
    56341 tatatatgat tatttatatc atgataaagc ttcttaactt tagcgtgtga ttcttgaata
    56401 cttagataat gagaatactg catatctagc ttatttttta ttacttcatt ttgcgatttt
    56461 aacttatttt ccttaattgt ttttataaac actataataa ttactaagct actagctagt
    56521 attaacctag gtatcacaat atacccaact atatatattt cctaataccc accaaatcga
    56581 tacatttttc tatttatata aattattaat atagtaaata aatttgttat taaagcaaca
    56641 gataagtata tatagtttat tttattatct ttatctagtc ccaatctttt attaataaat
    56701 ttaaatatag cacaagctac tatataacta ttattataag ataatagtat atatctactt
    56761 tagcattatc ataactaaac actaatagag cttcctctga tgatattaca ttacttgtaa
    56821 ctacaaaaaa taaatttata gatataacat cttttattaa agggtgatat acgtcagata
    56881 agaaaaatga aactatatat ttaacaattc ctatcttata aaaataatta tagtaagttc
    56941 ccaagtaata aatatcaaat aatagattat atcatataga tttttcttca tattataatc
    57001 atgggtctta tcaacgttaa ataatataat gattttagtt ataagataag ctaataatac
    57061 agtaaaaaaa gatattttct tagtcttatc tgtatcttta tcgtaagtta atatactttt
    57121 tagtatcatt attggtgtaa acaatgataa tattaataat attttattta tatggtttaa
    57181 catattctat caccaataaa ttttagaaac ccctctttta tatctttata tctatatcta
    57241 ctaataggta cttcttcttg actgtctaat attgcagtat ttgactttat attttctaca
    57301 tagtttaagt taattatata acttttatga catcttacaa gttcctctat atttaaatct
    57361 ttttctattt tttctagtat acatatagca tcaaaagttt tattttttgt atgtattagc
    57421 atgttctttt tttgaacttc tatatatttt tttgctagat tagattttgt atgtgttaga
    57481 cttattttta tttttttcaa tttttttgat acatgttaat atgtgttttt ctagttcttg
    57541 aaaatttatt ggctttaata agtatcaata agctcttact tcatatcttt cctgtacata
    57601 atttattaga gatgttataa atattattat ctacttttct tatgtttctt gcgatatcta
    57661 tactatttag ttctgccatt tttatatcta agagtagtgt atcctctcaa ttaactttgt
    57721 aatggttctt cttttgagct atattttttt aattcgatat tctttgtttt tattttttaa
    57781 tatattatca acttgatttt ttattacgat ttgtatttct ttttcatcat cacaagctgc
    57841 tattgtaatg atgacctctc ctagtattgt atttttattt agattattca ctttaggtta
    57901 acacttttta aatgtattta tcaataatat ttaaattaaa aatgtataaa aatacctata
    57961 tccaattgga tataggtatt cgtggatttc aatggtggat acgaggggag tctataagtg
    58021 gttatttgat tgttttgttg gttttgtaaa agttgataat acagcagttt acatataata
    58081 ctatataaaa tttttataat ttttttgtta tttgttttta taattggggt atagttgggg
    58141 tataattata taaagttatt acacactata atattaaact attttacacc ttttgatagt
    58201 gatattacat agtcattatg tatttacctg taaaaaatat tttattgtta tcaattggac
    58261 atatgttatt ctcatcttct agtactttca tagtttcttt atacatcaat tttaccttta
    58321 taaaaacttc ttctgttaag aaacaatttt aagttcaaac agaatcaaac atatcataaa
    58381 ccttgataat gctaaattac aaacaatcaa tataagaaat attcacttat aaaattatca
    58441 tttttacttt aattaataat taaaccataa aagtatatta ataatatcat gctaattttt
    58501 tgtatctctt ataagttttt caagtccatt aaaattaatt atctttattc tattattatt
    58561 taacttagag tttatggcca tattatatgt tttaaatact ataattggct gaatctcttc
    58621 aatttcacaa ccaaatttaa tttcaagatt ttgtttattc tctaatacag atttatattt
    58681 tttctccatt ttacttataa atgaatatgt tttgtttata tcacatttaa atccatatgg
    58741 tgtaaattta taatatatgt ccttacattc tataataaat aatttttttg taactactgc
    58801 aattaagtca atctcattag ataatgttat aatcttttca ttgttaatat aaactttatg
    58861 agcatttatt ataattttat ttgtatattt cttcaatata ttgctacaat ctataactag
    58921 attatttttt atttctcttt ctataatttt tgagttaatc ttcttgcatt ttggaagaag
    58981 attataaacc aactttctta ttaaaatttc ataggaatgt
//
LOCUS       DAMJYC010000014        56895 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJYC010000014 DAMJYC010000000
VERSION     DAMJYC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.015"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..618
                     /locus_tag="PC472_000936"
     CDS             <1..618
                     /locus_tag="PC472_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF2726282.1"
                     /translation="ADGATGPTGATGATGVTGATGPTGPTGATGATGASAIIPFASGI
                     PLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITS
                     ISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSIS
                     SGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            787..1548
                     /locus_tag="PC472_000937"
     CDS             787..1548
                     /locus_tag="PC472_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2726283.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1857..2135
                     /locus_tag="PC472_000938"
     CDS             1857..2135
                     /locus_tag="PC472_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726284.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2428..3003
                     /locus_tag="PC472_000939"
     CDS             2428..3003
                     /locus_tag="PC472_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2726285.1"
                     /translation="MKKITWEVEYISRLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            3442..4152
                     /locus_tag="PC472_000940"
     CDS             3442..4152
                     /locus_tag="PC472_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726286.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            4146..5462
                     /locus_tag="PC472_000941"
     CDS             4146..5462
                     /locus_tag="PC472_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2726287.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            5474..5614
                     /locus_tag="PC472_000942"
     CDS             5474..5614
                     /locus_tag="PC472_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2726288.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            5673..6254
                     /locus_tag="PC472_000943"
     CDS             5673..6254
                     /locus_tag="PC472_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2726289.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            6401..8110
                     /locus_tag="PC472_000944"
     CDS             6401..8110
                     /locus_tag="PC472_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726290.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSYMAYKINLSSKSNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            8125..8997
                     /locus_tag="PC472_000945"
     CDS             8125..8997
                     /locus_tag="PC472_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2726291.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            8998..9786
                     /locus_tag="PC472_000946"
     CDS             8998..9786
                     /locus_tag="PC472_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726292.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(9882..10520)
                     /locus_tag="PC472_000947"
     CDS             complement(9882..10520)
                     /locus_tag="PC472_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2726293.1"
                     /translation="MIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQ
                     KTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDK
                     AVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRAKKDNRFL
                     EALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLEK"
     gene            complement(10547..11176)
                     /locus_tag="PC472_000948"
     CDS             complement(10547..11176)
                     /locus_tag="PC472_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2726294.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            11482..12141
                     /gene="cprR"
                     /locus_tag="PC472_000949"
     CDS             11482..12141
                     /gene="cprR"
                     /locus_tag="PC472_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2726295.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            12352..13410
                     /locus_tag="PC472_000950"
     CDS             12352..13410
                     /locus_tag="PC472_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2726296.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            13661..15805
                     /gene="fdhF"
                     /locus_tag="PC472_000951"
     CDS             13661..15805
                     /gene="fdhF"
                     /locus_tag="PC472_000951"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14075..14077,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF2726297.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            15854..16687
                     /gene="fdhD"
                     /locus_tag="PC472_000952"
     CDS             15854..16687
                     /gene="fdhD"
                     /locus_tag="PC472_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2726298.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            16698..16871
                     /locus_tag="PC472_000953"
     CDS             16698..16871
                     /locus_tag="PC472_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726299.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDENN"
     gene            16908..17459
                     /locus_tag="PC472_000954"
     CDS             16908..17459
                     /locus_tag="PC472_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2726300.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            17484..18860
                     /locus_tag="PC472_000955"
     CDS             17484..18860
                     /locus_tag="PC472_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2726301.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            18860..19375
                     /locus_tag="PC472_000956"
     CDS             18860..19375
                     /locus_tag="PC472_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2726302.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(19453..20736)
                     /locus_tag="PC472_000957"
     CDS             complement(19453..20736)
                     /locus_tag="PC472_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2726303.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            20924..21346
                     /locus_tag="PC472_000958"
     CDS             20924..21346
                     /locus_tag="PC472_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2726304.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21448..22386
                     /locus_tag="PC472_000959"
     CDS             21448..22386
                     /locus_tag="PC472_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2726305.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFAAEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            22572..24596
                     /gene="ligA"
                     /locus_tag="PC472_000960"
     CDS             22572..24596
                     /gene="ligA"
                     /locus_tag="PC472_000960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2726306.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKKIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            24756..26105
                     /gene="rph"
                     /locus_tag="PC472_000961"
     CDS             24756..26105
                     /gene="rph"
                     /locus_tag="PC472_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2726307.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            26105..26575
                     /locus_tag="PC472_000962"
     CDS             26105..26575
                     /locus_tag="PC472_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2726308.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            26769..28055
                     /locus_tag="PC472_000963"
     CDS             26769..28055
                     /locus_tag="PC472_000963"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2726309.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            28205..28789
                     /gene="clpP"
                     /locus_tag="PC472_000964"
     CDS             28205..28789
                     /gene="clpP"
                     /locus_tag="PC472_000964"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2726310.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            28808..30058
                     /gene="clpX"
                     /locus_tag="PC472_000965"
     CDS             28808..30058
                     /gene="clpX"
                     /locus_tag="PC472_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2726311.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(30129..30656)
                     /locus_tag="PC472_000966"
     CDS             complement(30129..30656)
                     /locus_tag="PC472_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2726312.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(30644..31156)
                     /locus_tag="PC472_000967"
     CDS             complement(30644..31156)
                     /locus_tag="PC472_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2726313.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            31336..33699
                     /gene="lon"
                     /locus_tag="PC472_000968"
     CDS             31336..33699
                     /gene="lon"
                     /locus_tag="PC472_000968"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2726314.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            33689..34300
                     /locus_tag="PC472_000969"
     CDS             33689..34300
                     /locus_tag="PC472_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2726315.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(34565..35935)
                     /locus_tag="PC472_000970"
     CDS             complement(34565..35935)
                     /locus_tag="PC472_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2726316.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(36110..36225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            36513..37592
                     /locus_tag="PC472_000971"
     CDS             36513..37592
                     /locus_tag="PC472_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726317.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            37983..38663
                     /locus_tag="PC472_000972"
     CDS             37983..38663
                     /locus_tag="PC472_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2726318.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            38656..40035
                     /locus_tag="PC472_000973"
     CDS             38656..40035
                     /locus_tag="PC472_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2726319.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            40206..41291
                     /locus_tag="PC472_000974"
     CDS             40206..41291
                     /locus_tag="PC472_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2726320.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            41345..41704
                     /locus_tag="PC472_000975"
     CDS             41345..41704
                     /locus_tag="PC472_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2726321.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            41757..42629
                     /locus_tag="PC472_000976"
     CDS             41757..42629
                     /locus_tag="PC472_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2726322.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            42630..43073
                     /locus_tag="PC472_000977"
     CDS             42630..43073
                     /locus_tag="PC472_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726323.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            43088..43672
                     /locus_tag="PC472_000978"
     CDS             43088..43672
                     /locus_tag="PC472_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726324.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            43925..44962
                     /locus_tag="PC472_000979"
     CDS             43925..44962
                     /locus_tag="PC472_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2726325.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            45043..45408
                     /locus_tag="PC472_000980"
     CDS             45043..45408
                     /locus_tag="PC472_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726326.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            45413..45730
                     /locus_tag="PC472_000981"
     CDS             45413..45730
                     /locus_tag="PC472_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726327.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            45778..47382
                     /locus_tag="PC472_000982"
     CDS             45778..47382
                     /locus_tag="PC472_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726328.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            47506..49134
                     /locus_tag="PC472_000983"
     CDS             47506..49134
                     /locus_tag="PC472_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2726329.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            49399..50748
                     /locus_tag="PC472_000984"
     CDS             49399..50748
                     /locus_tag="PC472_000984"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2726330.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            50904..51983
                     /locus_tag="PC472_000985"
     CDS             50904..51983
                     /locus_tag="PC472_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2726331.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            52328..53236
                     /locus_tag="PC472_000986"
     CDS             52328..53236
                     /locus_tag="PC472_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2726332.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            53241..55610
                     /locus_tag="PC472_000987"
     CDS             53241..55610
                     /locus_tag="PC472_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2726333.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            55935..56738
                     /gene="proC"
                     /locus_tag="PC472_000988"
     CDS             55935..56738
                     /gene="proC"
                     /locus_tag="PC472_000988"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2726334.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        23244 a         5958 c         9956 g        17737 t
ORIGIN      
        1 gcagatggag caacaggtcc aacaggagca acaggggcaa caggagttac aggagcaaca
       61 ggcccaacag gcccaacagg agcaacagga gcaacaggtg ctagtgcaat aatacctttt
      121 gcatcaggta taccactatc acttacaact atagctggag gattagtagg tacacctgga
      181 tttgttggat ttggtagttc agctccagga ttaagtatag ttggtggagt aatagacctt
      241 acaaacgcag caggaacatt gactaacttt gcattttcaa tgccaagaga tggaacaata
      301 acatctattt cagcatactt cagtacaaca gcagcacttt cacttgttgg ttcaacaatt
      361 acaattacag caacacttta ccaatctact gcaccaaata actcatttac agctgtacca
      421 ggagcgacag ttacactagc tccaccactt acaggtatat tatcagttgg ttcaatttct
      481 agtggaattg tgacaggatt aaatatagca gcaacagcac aaactcgatt cttactagta
      541 tttactgcaa cagcttcagg tctttcatta gttaatactg tagcaggata tgcaagtgca
      601 ggaattgcaa taaattagat tagataagaa aaggcttcta atatcgtatt gatattagga
      661 gcttttttaa gaatgattga tgaacatagt tttaatatta attagtgcgc atattataaa
      721 atactattat ataatataaa ataataacat taaattggaa atatagaatt atctaggggt
      781 gatactatga aaaataagat tgaaatatca atgaacaact ttgtaaaaaa ttatcataaa
      841 tcaaataata taactacaaa gtggcaagaa cttttgtttg cttatgcatc tgctgaagat
      901 gatatgtttc agaagctaaa aagtgttgta ggaacatctc atgctatgcc aaaagatttt
      961 ctggaaaatg gaaaaactat aattacatat tttatacctt ttgatgaaag tgtaaataat
     1021 agtaatattg gagggtttga atgttctgaa atatgggcaa gagcgtacat agaaactaat
     1081 atgttgattt caaatttaaa tgatttctta tgtgaagaac tagagaagat aggggctaat
     1141 tcatatgcta ttcctgggtc gtataatttt gacagtgaaa aattggtaag tgactggtca
     1201 caaagacatg tagcattcat agcaggtctt ggaacttttg gtttaaataa tatgcttatt
     1261 acagaaaaag ggtgttgtgg aagaatagga agcataatca caagtttaga attagagcca
     1321 actaaaagac cagaaaatga atactgcttg tataaacata ataatagttg caaaaaatgt
     1381 gtcaataaat gcgtgaaaga agctttaaaa gaagatggat ttaataggaa taagtgttat
     1441 gaaatgtgta tgataaatga aaaaaaatac acttatatag gtggatgtga tatttgtgga
     1501 aagtgtatag ttggagttcc ttgttcagta ataaacccat caagataatg gatttaagtt
     1561 gactaataat tttaaagata gtttataatg aaaaaatttt agttatcaaa ataaatataa
     1621 tataaaaact aaataaatgt gtttaaaaaa tggataaata atttgtgttg attgacaacc
     1681 agttatactg ttgatagact tatatgtatc gctgtgatat ataaggaggg ttgagatttt
     1741 atgtttgatt taatctttat ttttgcatag caagggctat tgcaactatg aaatttaata
     1801 tataaatagc cctttgctat tcctaatgaa aaattactat ttaataggag agcaaaatga
     1861 gctataaaaa agcaatacat atattaccag aagaattact tgaattaata caggaatatg
     1921 tagatggtga atgtatctat attcctagaa aatctaataa taaaaaagaa tggggaagta
     1981 atacacctac tcgtgaagag ttgtcaatta gaaatatgca gatttacgaa gactatcaag
     2041 ttggatatga tttgcaatat ttatcggaaa agtattattt atctttgaaa agtatccaaa
     2101 gaattgtatt acaagaaaaa agaaaagata tctaaggatg agccagtata gttttataat
     2161 tatactggct cattttcttt tgataaaagt gtttgaggta tagtttcttt taagtggatg
     2221 atataattta agtaatttca ataacttata cagtagctaa caaagctggt tgtaaagtat
     2281 ctaataagaa gtagtagctt tttatatgtt tgaaattaaa atttattaaa attaggaggt
     2341 cttgttttga aaaaaatact tgattatatt ccaccttttc ataactttct attaatttag
     2401 taatcttata gaaaggaaat tgtatttatg aaaaaaatta cttgggaggt ggaatatatc
     2461 tcccggttac ctgtaattag gtattgtaaa aaatgtggaa aaaagactga atatatttgt
     2521 tcaggtttat ttagggttaa tgctcaacat aaatatttgg atatatggtt aatatacaag
     2581 tgttcaaatt gtgattcaac atggaactca actatatatt ctcgtgttaa tccaaaaagc
     2641 ttaaatccag aaatactaga acaattccat acaaacgatt ataatcttgt taagcaatat
     2701 gctatgaata tggaactttt acatagaaat ggagcagaag ttggtattcc agagtataaa
     2761 atagtaggac aagaaattaa tattaatgac cttacacaag ttcatattat tagtaaatat
     2821 ccatgtcagt taaaggtatc aacattatta cgtgagaaac taggtttatc taggaaggta
     2881 tttgacgaaa tgttggattg tgggattatt agaagtaact caggagttga tttaaagaaa
     2941 ggtaaattaa attatgaaat tatgttagat ataggcaagg agaagcaaaa gtcttcaaat
     3001 tgattagaat aactaactta atatattttc aggaacatca tttaaaaaaa tagtggcttt
     3061 aaaaaattta aagccactat tttttaatat gttttatatt aatatttaaa atatagtgtg
     3121 tattcttcaa aattatagcg ataattaaaa tattactata aaaaggaagg tgcatacttt
     3181 ttatttaaca aaaactagat attaaaaact tttcgaggaa tattttatta aaatccctaa
     3241 atatatttaa tatatattaa aaaattgtat tataatgaaa atcaaaatag attgcaaatc
     3301 caaaaagtaa tacttaatgt ccaaaaagct aaaattaaaa tctaaatagc tatttactac
     3361 agaagtatgt taaaatatgt aaaatgaata gaaatttata tatttttatt aaaaatatat
     3421 aagaaaggat gtttaagaat tgtgattaat ataggaatat gtgatgatga actacattat
     3481 cgaataaaaa taaaagatat attaagtgaa atattaagtt catatcctat aaattataac
     3541 atatatgaat tttcagcagg agaagagtta ctaaataact atcctaaaga tttagacata
     3601 ttaattatgg acatacaaat gaaaacaata aatggtatgg atactgctag gaagataaga
     3661 gagtttgatc ataagttaga aataatattt gttacttcat ttgtagagtt tatgcaagaa
     3721 ggttatgagg taaaagcata cagatatata ctaaagccaa taaataaaga aaaaatttct
     3781 aaaagtgtat taccttgtat aaatgagatg atgaaaaaga gaaataatta cttaactata
     3841 aatgtaaaaa attatgtaga ccgaattaaa atagattcta ttacttatat agaaacagat
     3901 agaccgaata tattgattta tacacatgat gatatgtata ttactaaaat gagtatatct
     3961 aaaatagaaa agatattaaa tgaatatgga ttttttagat gtcataatag ctatatagtt
     4021 aacttaaaat tagtagaatc aatgagtgga agtactgtta tagtagatgg aaaaagtatt
     4081 ccaataagta aatatagggt taaaggcttg aagcttgcga taactaatat attaggggac
     4141 atagtatgct aacaaattta agtgtaggat tttggaatat tattaattta gtttcaggta
     4201 ctatagattg gattatattt tttttagtaa tccacttagt tggaaagagg aaaatatcta
     4261 atataagata tatatttaca acaataatta taattatatt tatggcatac ataaatgtag
     4321 ttaaagtgtt tccaaatatt aagattcttc tatgtatggt aataggaata tcattttata
     4381 taacatcata tgaagataaa atctataaaa gtataatagt tagtttattg ttttggctag
     4441 gattaatggt tgctgaaggt tctgcagtag gtctagtagt atttataaat aaattaaata
     4501 atattaacat aacacaacat gaaaacttat ttagaataca agctattatt atgtcaaagg
     4561 tatttttatt tataggtttt atattgttta agtattttaa attatcttta gattttaaac
     4621 ctaaagatat gatactaata ggaataccta tattatctaa tatagttagc ttattactta
     4681 tatttgaata taatttaaga gtcaatacaa ttactacaga aaacttattt atatttatat
     4741 ttgcaatttt attgatttta tcatcaagta taatactttt aatagtaata gggaaaattg
     4801 tacaagacga taaattaaat ctagagtatg aacttataaa tgaaagaata aaaacaaact
     4861 acaggaatta tgaaaatata aacgaagttc atgatagatt aaaatatgtt tatcatgact
     4921 tgaaaaacca tatgatttgt ataaaaaatt atgacactaa agaagaaatt atatcatata
     4981 taaataactt agagtttcag ataagtgaat ttgaacattt taaaaatact ggaaataaaa
     5041 cattagattt aattttagca gaaaaaataa gtatttgtaa aaagtatgat attcaaattg
     5101 aagataacat aaatatatct aaattaaact ttattaaaaa taatgatata tgttcaatat
     5161 ttgcaaattc actagataat gcaattgaag catgtatgga tataaataat gagatagaaa
     5221 aaagaataga agttaaagct acatatataa ataaatttgc aataataaaa tttataaata
     5281 ctaaggtaaa tgatattaaa cttattgata aacgtattca aactagtaag gatgatgata
     5341 agatacatgg aataggactt gctagtatta aatatatagt aaataaatat ggtggtgaaa
     5401 caatagttaa ttactcagat aatgagttta tattaaaaat aatgatacca ataaaaagtt
     5461 aggagagtac aaaatgaaaa aaatagcgtt aaacttactt aaaaacatct cagcattaag
     5521 ttttgggata gctgtattat cagcaaacag tgcaagttct tgggtagcac atcaggcaaa
     5581 agagccacaa gctttacaaa aactaaaaaa ataacattaa taaaaggacc tcttcttatt
     5641 aaaggagagg ttttacatgt tgaggaggaa atatgtttaa aagattatca tataaatttg
     5701 ccaatatatt agttaataat gagattgtag aaagtgaaga ttttgaaata tatagatatg
     5761 gatttgaaac tttgatttat ttcatagtta atattttggt agcattattt ataggaatta
     5821 tttttgatag atttatacat acagttatat ttttaagttg ttattgcact ttaagacagt
     5881 tcacaggagg ttatcatgct aggaattata aagagtgtac attaacattt gcagttattt
     5941 atttgataac tattttttca gctaataata tagatataaa taaatataag tatttattag
     6001 tattattaat gattattagt atactaacaa tttacaagtt agctccttta gaacatagaa
     6061 acaaacctct tagtgaaagt gagaaaaagc attatagaaa gactgtgcaa aaaatactat
     6121 tcgtaataat atgtttaatt attttatgta aaatacttaa tatttttcag caatatgtga
     6181 tttatgcaat aatttctata tattggattg ctatattaat atatattgga atgaaggtta
     6241 atagtgatca gtaagattat aaaacatttt ctaaattaat taacttcact aattaatatt
     6301 aactttaaaa aaataatgtc aaggtaagta tttgaactta ttaaatatag ttgaatctat
     6361 tgtaggttgt atcaaaagac tggctaggag gggcataaat atgagtagta ttaaggttat
     6421 atcagacttt ataaccaaaa taattagaaa aaatatttta tttgcaataa caattattat
     6481 tataaattac catctcatac aacgatggac acatatatcc aatagaggaa tttcatttaa
     6541 tgattactta tccttgagtg tatatacttg tatgacaatt agtattttaa ccatgttaaa
     6601 tgtaattttt tatatgagaa aaagttatga tactgtaaaa atattatgta gaaataattt
     6661 tgattatttt ttaggtattt ctgttgctgt aactagaaac aactttatat tttgttgcat
     6721 accaattata tatgctatag catactcaag tttaataaga acattgtcaa ttgtaacgat
     6781 taaatcaact atatttatat tgatatttga atggttttta ccacttacta ttttatctat
     6841 agtgatatca tttatttcag ttattataaa taatttaata ctaaagatta ccttatcatc
     6901 agtattttta tatttaacat caactaagat gttggataga gttattgata ttaaagaacc
     6961 attttttaaa gcattattta aatcacttaa catatttgaa gatcaatcat atgcttacta
     7021 ttctgatttt gtagggaaag ttaataattc agcatatgtt atagataaaa ttatacctac
     7081 actatttgca attactttaa taatagtagc atatctgata ttaaatgata ttagaaagaa
     7141 acagattaaa tcattaatag ttatagtagt gtacagtatt ttatttattg gattaaatta
     7201 tatatcaata gatatgtcaa catatagaga tgaggagcgt agttatggag agtatttaaa
     7261 gaaatctaat attagtgata ttaaaataaa atcgcatgat atgaagattg actttaaaag
     7321 caagtcaaat ataaaagata gaattgtttt attaaacaca tcaaatgaag agttaacaaa
     7381 tattaatatg attttagatg aaatatttga tataaaatat ataaaagtaa attcggaaaa
     7441 tataaaattt aacaaaaaaa aggatttaat tgaagttaat ttaaaggaaa gtttagagcc
     7501 taatgacgag gtgacattag atatagagta tgaaggctat attaatgtgt tgacatcttg
     7561 gaatcaaaat aaatatatag ctactaaaga tgatattatg cttccaccag gttctttagc
     7621 gtggtaccca aaagtaaatc aatcaagtta tatggcttat aaaataaatt taagttctaa
     7681 aagtaatgtc tattcaaatt tacctttact gaataagact agtaattttt taaaaaaaga
     7741 ttatatattt gaaggaaagt caaatgatat tgcaatatat gcaggtgagt ttaaagaaga
     7801 ggtaaaagat gatataaaaa taatctatcc atcagatagt aatgtaaata tggatataga
     7861 ggaaaaaatt aaatttttaa ttgaaacaag aaatgaaata ttaggtgata gcaaagcatc
     7921 tgtagatgct aaaaaatatg tagatgaagt agataatgtt atggaatcaa taaaaagaaa
     7981 aaaaattaat atgatattag ttgctgactt acctagcaat gaatttttat atgaagactc
     8041 aaagggagag aaaattagca taactattta tcattggctt tcaggaaata tccttataat
     8101 agataaataa taaaggaggt taatatgata aacaagttag aaataagaaa tctaaatttt
     8161 aattacaaaa ataaaaaagc tttggataat ataaatttaa ccctaacaga tggagtagtt
     8221 gcattattag gtcctaatgg tgctggaaaa tcaacattaa tgaggttatt agtgactctt
     8281 tatgaaactt caattggaga gatagaatta aataatataa agtatagcaa aaacaatgag
     8341 aaaatcaagg caaatgtagg atatgtacct caagattttg atatgtataa taatataaat
     8401 gggcaagaat acttagaatt tgtagctaaa atgagaggtg tatctaagaa tgatttaaag
     8461 aaacatatac aaaaagtagt atcaaaagtt aatctggaca agtttataaa taaaaaaata
     8521 ggtacatact caggtggtgt aaagagacga ctaggtatag cacaggcttt aataggtgat
     8581 tctaagttaa tagttatgga cgaacctaca gttggtcttg atccagagca aaggaatgaa
     8641 tttagaagat tgttaccaga aataagtaaa aattctatag tattaatttc aactcacata
     8701 gtagaagaca tacaatttaa ctgtgacaaa ttaattatat taaatcaagg caaaatatta
     8761 tatgatggta caattaataa gtttattgat atggttgagg tgtattcggt agtagtttct
     8821 aatgatgaat ttgaatattt agaacgtaat attgatatta ttgattatag gagagttaaa
     8881 gatggtgtgc aagtaaagta tataggtaaa aatacagaga aacttaaaga tagcttaagg
     8941 atagatacaa ctttacaaga tgcttatata aactttttac ataaggagtt aaaataaatg
     9001 tgtatattgg agttaataaa atttgagctt aaaaaaatag agcttaaaca tgtgttaata
     9061 tttgtaatta tttctatctt atgcatattt attacaggca tattagatta taatgagtta
     9121 atattttata atgatgtacc tataatggat acatattcta tagcaggatg gttaggaatt
     9181 ataaagatat cagatgtttt tataccaata atgtcagcaa tcacaataat ttatgtgttt
     9241 tcaaaagatt attcgggtaa tataaacgag atacttactt tatataataa aaaaaaatat
     9301 aatcaattta tagtatttag atggtgtgtt atattagcta tatatactgt attattatta
     9361 atttcaatta caatagtata tacatttacg tcagtgaaag gttttaacat agacaaaata
     9421 gattttgttt atagtgtaaa gccaatagat gtgtttttaa agacatttcc tacattatta
     9481 tggtatacaa catttccact aatgatatta acattaacaa aaaatagatt tacaacaata
     9541 gcaatattaa ttacatatac atttgctgat atatttttta ttttacatat gtacccattt
     9601 gttagtatga ttaatgtaaa tggattttat attcaaaaga tgttttatat gcctaacaat
     9661 atagatgtta gatttaatga attaaagaat tatttcttta taaatagagg ttctctagta
     9721 gttatttcta taataatagt tttttatata gcaagaaaat caacggtttt aaaaaagagt
     9781 aaatagttta atatattttt tatattgtta tatttatata gaatagtgac tttaaaaaat
     9841 ttaaagccac tattttttaa tatgttttat atatataact attatttttc taggtttttt
     9901 aatatttcgt aatagtgtgt tgtggctatt tcacttgact cgcctttttt acaaaaagat
     9961 aatttggata gttttggaac tacacgttca acgacaattt tattacctat cattttagat
    10021 tcaagtatct tcaatgcttc taaaaatcta ttatcctttt ttgcacgatt ataaaatgat
    10081 aaaacgtaga cataaaggaa taggttataa ttacggaaag gatattcaac ttgcataaat
    10141 aatgtgccaa ttccatagtg acaagggccg attggttttt taatagtcca atggtcaagt
    10201 agaaaatcta cagctttgtc aagttttgat tctttattta gataattact aaaacggaat
    10261 gcatttaata tattaagagt tggtagtgga tttgagtatt cagtttctgg acctcgacca
    10321 aaactaaatt tattgcaacg ccacccacca tcagtatact gtgtatctaa aaaatgttga
    10381 aacgtttttt gcattctaat gtcagatgca tatcccatat gacagagtac gttagcagca
    10441 tttgcagtat gacaagggta aatacctcca tttggatata gtttgaaacg tccatcttct
    10501 cgccatgtgc taaatatcaa aaactttatt ataatattat ttatatttat aattgattta
    10561 ttttatttaa aattgcattt atagctgtat caggaacaac tgctaaatct tctgcattga
    10621 agtaataaac tatattagaa tcaaataata tatttgaatt tggaggaaat tcatcatctc
    10681 caaaccaaag tataaatatc atagatgtat tacccatata tgtaaaccta taagctaaat
    10741 ctcccatgtt tactttttca gctccaattt tctccataac taacttatat ttgtctattt
    10801 gagatgtaaa aacttctgaa agcctatata gagtcctgtt taaaaaattt ggataataaa
    10861 cattgcctcc tggtatttct ttaaaagtta catatttatt agttctagga atacctttag
    10921 cattgattaa atatctaact atcaagattt taagctcaaa gttattgcaa aggctaccat
    10981 tactattcca aacttctcca cttggatagt ctataatata ttgtctatca aaaaaattga
    11041 gtgtgaatct atttaaatca ttattgaaag gtatattact taaattacta attgtatgag
    11101 ggtctaattc tttgagtttt tttcgaacat agtcaaatgg aacattataa ttattttttg
    11161 ttatcttttc atccataatt ttttcctcca atttaaacaa ttaaaagtat taaaaagttt
    11221 tcttattatt aattttatta tatattcaaa atattacaat aataaattgt taaataaatt
    11281 tttatattta agtaaatacg tgtaaaatga atataaagtt tttgaaatat aaagtttttg
    11341 tatctaaaat attttatttg taaaatagta attaattatt gtaagctata atatgagagt
    11401 attatggtta atgtgaattt taaagatatt attaaatata taaattagaa tgctattaga
    11461 tggataaatg gaggagttat tatgtctaag atattagtag tagatgatga gttagatatg
    11521 ttaaagttga ttgaaaatgt gttgaaaaga gatggacatg aagtaaaggt aattagtgat
    11581 gtagaaaagg ctatgaacat ggattttaat tattttaatc taataatatt agatgttatg
    11641 atgcctaaga tggatggttt tgaagtatgt aaaactataa gaaataaggt tgattgtcca
    11701 atactgtttt tgacagcaaa aaatatggaa agtgatatta tgtacggtct tggtataggt
    11761 gcagatgact atataaccaa accttttggt gtaggagaat taagggctag gataactgct
    11821 catttaaggc gagaaagtag agaaaaaaag aatttattaa ctatctcaaa tgttcaattt
    11881 aactttttag gcaaagaagt ttcaattgat ggtaaaaaaa ttaattttac taaaagtgaa
    11941 tatctaatat gtgaatttct agcaaaaaat aagggacaag tattttcaaa agaaagaata
    12001 tatgagtcgg tatatggttt tgatggagaa agtgatataa gtactatcac agagcatata
    12061 aaaaatatac gaagcaaatt aaaacaatat gatatttata caatagaaac tgtatggggg
    12121 atagggtata aatgggtgta ataagtataa gtgttgttta gtgaaaatat tatatatatc
    12181 tacaaaatgt ttgtaaaata ttaatctata tattaaaatt taatttatag attaatattt
    12241 tttgtaaatg acaaaattgt aaggtatttt gttataaaat attaagattc atagtgtata
    12301 ctaataatat agtatttagt ataaatcttg agcaaaggat ggttatttct aatggataga
    12361 ctaaagaata ttagaaagag tttactacag tatacatttt tatttttgtt aataggtata
    12421 acaatttacc tagtgtttaa aacattggat attaaaatgc tatcaaatgt tatggttatg
    12481 gtagataaga agtttttatt cataggtgct ttggcaataa tgtgtcacat aatgcttgaa
    12541 ggagtagtaa tgaagataat tatagaaagc actcacaaag ttaatattag atttattggt
    12601 tttaagctag ccattatggg attttattat aatttaataa ctccttttgc ttcaggaagc
    12661 caaccagtac agatatatgt acttaaaaaa tgtaaaatgc cacttagtaa ggcaagtgca
    12721 gttgttacca ataagtctat tatcttccaa atagtagtta ctttttattg tacaatactt
    12781 gttttaatga attttgctat gttagaaaaa caaatgaaaa tgattatgcc tctagtgttt
    12841 ctagggatag ccattaattc atttgtctta attatgataa tattagttat tttaaatcct
    12901 aaaaaaataa aatattttat gagaattact ataaagcatt tatcaaaaat taagttttta
    12961 aaatttttag ataataagat tgaatccata gaaagtttta tagatgatta tagtaaagca
    13021 ataaattttt ttgcaaagaa taaaaaggta ttaatttcaa ctataatagt aacttttatt
    13081 caattaagtg cttactttag tgtttcattt tggatatata aagcatttaa tttacaaggt
    13141 tatagctata tttatatact gacattacaa gcatttttat atatggcaat atctccaata
    13201 ccaacacctg ggaatgtagg agctaatgaa ttagcctttt tcactatatt caaatcagta
    13261 ttccctcaac ctttgatggg atatgcagta tttttatatg gtggatttat gtactatttg
    13321 attctgattg gaagtggaat atttactgta attactcact atagaatgaa aaatagaatt
    13381 ggcagaaata ttgttttaag tgaaaactaa ataagtttaa agaatatata ttgagtatat
    13441 taaaaaatat gtcataagaa ctagaatatt ttaattaaat ggttgaaata taaagattat
    13501 tatgacatat tttttttgaa atttaagagg aattgtgaga aaattgttga atttaataga
    13561 tagtattgca aaatatttat gattttgcat attataaaaa cactcattat ctttaagata
    13621 aattttactt atcaggggat aaatctaagg ggggaattgt atggagaaaa aaattttaac
    13681 agtttgtcca tactgtggag ctggatgcca attatatcta gtagtaaaag ataataagat
    13741 attaagagct gaaccggcta atggtagaac aaatcaagaa agtttgtgtt taaaaggtcg
    13801 ttatggatgg gattttctaa atgaccctaa aattctaaca cctaggctaa aaaagccaat
    13861 gataagaaaa caaggtgtac tagaagaggt tgaatgggat gaggctataa actatacagc
    13921 ttcaagacta aatgaaatta aagaaaaata tggacctgat tctattatgg gaacaggttc
    13981 tgcaagagga cctggtaatg aagcaaacta tataatgcaa aaatttatga gagctacaat
    14041 aggaactaat aatgttgacc attgtgctcg tgtctgacat gcaccatctg tagccggtct
    14101 ggcttactca ttaggtagtg gtgcaatgtc taattctata ccagagatag aaaatgcaga
    14161 tgttttattt atatttggat ataatggagc agattcacat ccaatagtgg caaatagaat
    14221 agtaaaagct aaaaaaaatg gtgctaaatt aattgttact gaccctagag taactgaatc
    14281 tgcaagaatt gctgatatac atctacctat aaaaggtgga acaaacatgg ttttagtaaa
    14341 tgcttttgga aatgtattaa tagaagaagg tctttacaat aaagaatttg ttcaaaatca
    14401 tacacaagga tttgatgagt ataaagaaat tgttaaacca tatacagcta aatatgctga
    14461 aaagattaca ggaatcccag aagagcttat aagaaaagct atgagagaat atgcaaaagg
    14521 taaaaaagct atgatacttt atggaatggg tgtctgtcaa tttggtcaag ctgttgatgt
    14581 agttaaggga cttgcatcaa ttgctctact tacaggtaat tttggtagag aaagcgttgg
    14641 aataggacct gtacgtggac aaaataatgt tcaaggagct tgtgatatgg gagctcttcc
    14701 taatgtatat cctggctatc aaaatgtaac tgatgataag ataagagaaa agtttgaaaa
    14761 agcttggggg gtaaaattat ctcctaataa tggttatagt ttaactcaag ttccagactt
    14821 agtactaaaa gagaaaaagc ttaaagctta ttatatattt ggagaagacc cagtgcaaag
    14881 tgacccagat gcatcagaag ttagagaagc attagatgaa ttggaatttg taattgttca
    14941 agatatattt atgaataaga cagcacttca cgctgatgtt atacttccag ctacttcttg
    15001 gggagaacat gagggagtat atacatgtgc agatagagga ttccaattga tgagaaaagc
    15061 tatagagcca caaggagatg taaaaccaga ctggcaaatt ataagcgaaa tttctacagc
    15121 aatgggatat cctatgaatt ataaaaatac taaggaaata tgggacgaac ttagacaatt
    15181 atgtccaagt ttcttaggtg ctacttatga aaagatagaa actcaaggat gcgttcaatg
    15241 gccttgtaag agtgaaagta tggaagataa aggaactatg tatctgtatg aaggtcaaaa
    15301 gttttctact cctaatggta aaggtaattt gttcgctgct gaatggagac cccctatgga
    15361 agttgaagat gatgaatacc catttagttt atgtactgta agagaagttg gacactattc
    15421 agtaagaaca atgactggta attgtagaac acttagttca ttagaagatg aaccaggacg
    15481 tgttcaaata aattctaatg atgctgaaaa attaggaatt gaagatgatg aattagttag
    15541 aattagttca agaagaggaa gtgtaataac aagagcaaca gttactgata gagttaagga
    15601 aggtgcaaca tatatgactt atcagtggtg ggttggagct tgcaatgaac ttacaatagc
    15661 aaatttagac cctatatcta aaacaccaga gtataaatat tgtgctgtta aacttgaaaa
    15721 acttgaagac caagaattag cagaaaaatg tgtaagagaa gagtatcaaa gtttgaaaga
    15781 caaaatgaca gctacaaata tctaatgatt tattagattt aagatttata aaaggcagaa
    15841 aggctaatat tttatgaaca atatatactc aaaaactaaa ttacaagaaa atagtaagga
    15901 taaagttttt gaaaatagtt atagtctagt ggactacaaa tatttaaatt cttttaatgt
    15961 agaagtaagc aaaataaatg aacatgaagt atcaatagat aatgaagaaa taattgcaga
    16021 atatccactt agttttattt taaatgataa atatataaat acatttttat gtaccccata
    16081 taatttagag gaacttatag taggattctt gaaaacaaaa ggatacatag aaaacaaaaa
    16141 aagcttatta aaatttgaga taaatgagag tgaacgtttt gcaaaagtaa ctattcaaga
    16201 gaaacaaaaa aatcaagatg agcaaattat atttttaaat tcactagact atataacatg
    16261 tgctcctgta aagagcaata taaaaataaa ttatgatacg atttacagta taatggataa
    16321 aaatttaaat tcatcagaat tatttaaaaa tactggtggg gtacatagcg tatctgtatt
    16381 taattttgat aaaaaaatag tcacttgtga agatgtagca aggcataatg caatggataa
    16441 ggctattgga ttttgtgttt taaatgacat aacattaaaa gataaaattg tggctgtaag
    16501 tggaagaata tcattggaaa tgatattaaa agcagctatg actcaaattc ctattgtagt
    16561 atcaaaatca gcaccgacta atttatcaat agagctttct aaaaaattaa atataacgct
    16621 tattggattt gtaagaggaa gacggatgaa tatttatgca aatccacaga gagttatcaa
    16681 aaagtgagaa tgaatatatg tgtactaaaa aagaagagca aagatattca aagtgtgaaa
    16741 tctgtggagt attaattgat gatactgata aggtatataa taacacatac aatttgtgca
    16801 aaaaatgtaa acgaaatata agtactgaga aaatagtgaa atatataaat atatatgatg
    16861 aaaataacta gagggttgga aatatacaaa ttgaaaggtg ggataatatg aaaaataaaa
    16921 taggctcttt tgtaatagca gacccaaaca aatgtacagg ttgtagagct tgtgaagtag
    16981 cttgttttac aatgcataat cagcataata atgtgggtta tacagttgga acagttgata
    17041 tacctgttat acctagactt tacttagtaa aaggtgacaa tttctgtatg ccaatacagt
    17101 gtagacattg tgaagatgct ccttgtttaa actcatgtcc acaaaaagct atagttaaag
    17161 aaaacaatat tatgtctgta aatgaagaaa agtgtatagg atgtaaaact tgtctgctag
    17221 catgtccttt tggagctata gacttacttc cacaatatga agatggtaaa gaagtagaac
    17281 aagtaatttt agatgaaaat aagaagatag cttataaatg tgacttatgt aaagacaatg
    17341 aaaaaatagc ttgtataagt gcatgtcctc aggaagcatt aaaacttgtt actccaatag
    17401 atgacaaaaa agctaagaat agacaagctg cacttagttt attgttaaca aataaataaa
    17461 ggatacaggg gtaggtgacg tttatgataa taaatataga taaggattta tgtactggat
    17521 gtagagaatg ttcaaaggtc tgtccagtaa atgctattga aggtgaagag ggaaagccac
    17581 aagagataaa tttagataga tgtgttatgt gcggtcaatg tgttcaaact tgtaaatctt
    17641 atgcctcagt tatagatgaa ggatttgagt ttttacaaga gaaaaaacaa gaaagagaga
    17701 taccagagtc tataaatgaa cctatatttg cagcatacaa tgtatgtgat atagataagg
    17761 tgaagaaagc tttaagtgac ccaaatgtat tcactatggt ccaatgtgca ccagcagtta
    17821 gagttgcttt aggagaagat ttcggatatt atttaggata cttaggtgct gggaaaatgg
    17881 cagcagcact tagagcttta aattttgata gagtctacga tactaatttt gcagcagatt
    17941 taactataat ggaagaaggt agtgaattaa ttaaaagagt gacagaaggt ggaactcttc
    18001 caatgtttac atcttgttgt ccagcttggg taaaattcat ggaaaaaaat tatcctaaat
    18061 taactaatca tttatcttct tgtaaatcac ctcagcagat gggtggtgca atatttaaaa
    18121 cttatggtgc agagataaat aatgtagatg catcaaagat atttagtgtt tctataatgc
    18181 catgtacatg taaaaaatat gaatgtgata gagaagaaat ggattctagt ggatatagag
    18241 atgtagatgt agtattaact actagagaac tagcttattt aataaaagac atgggaattg
    18301 attttaaaaa cttaagagaa gaaaaatttg atagtccttt aggtagttat agtggagctg
    18361 gaactatctt tggtgttact ggtggggtta tggaagctgc tataagaaca ggatatgagc
    18421 ttataactgg agaaagtatt cctgatgtag aagtaaaaca agtaagagga gaaaatggtt
    18481 ttagaaagtc aactattaaa ataggagact tagacttaag agttggagta gtaagtggat
    18541 taaaaaatgt aataccagtg cttgaagact tggaaaaagg aaaattagat gtagatttta
    18601 ttgaagttat gacttgtcct gttgggtgtg taagtggtgg aggtcaacca aaaattttac
    18661 tagaagaata taaggaattg gcttatgaaa atagaacaaa agcaacatac gtgcatgatg
    18721 aacatttagc aattagaaaa tctcatgaga atcctgatat taaaaaactg tatgatgaat
    18781 atctggtaga accattagga gaaaaatctc atcatctatt acacactaca tattgtatag
    18841 gaaaggaagt ggctaagtga tgaactattt tgttatagca gaccctgata aatgcatagg
    18901 atgtagaact tgtatgatag gatgtgtagt tgcacattca gatgaagata ttttttacca
    18961 aaatccagat gaaataaatt ttaatccaaa actttctgtc ataaagacaa aagaagtaag
    19021 tgctccaata cagtgtagac attgtgaaga tgctccttgt gcaaaggcat gtccaaatgg
    19081 tggtatagtt agggttggta acacaataaa aataaatgaa gaaaattgta taggatgtaa
    19141 gacttgtatg ttagcatgtc ctataggtgc aatagacata gttacattaa aagatgtaga
    19201 tgaaggaaag ctttgcttta gagaaagaat gacagcaaat aagtgtgatt tttgtataga
    19261 atcgccagaa gggcctgcat gtgtaaatgt ttgtccaact aaggcattta ctatagttaa
    19321 ggatgaggat atagatagta aagttaaaaa taagagaaaa ttagctatat tgtaatataa
    19381 aaaagatgga gttttattta tgattaaaag ctccatcttt tttattataa tatttatata
    19441 tctaaaaatc aattattgca ttagtttatc taatctttct ttattcaatt ttttcattcc
    19501 tatagaagtt ataataagta aagtataggc aattataact gataaaatta taatagacca
    19561 agaaaatctg tatccaaaat tatcaactaa caatccaaat aatgttgaac ctaatgcaaa
    19621 tcccactgca aaaattaatt gtacgattcc atatatgcta ccaaactcct tttgaccaaa
    19681 gaaagagcca gttaaataag caggaccaat catataagca aatacagaaa gacctttcaa
    19741 aatggcaaat acaaatgcaa aagtagaaga tttatctacc aatagtaatg ataaagttgc
    19801 tataagaacc aatacaaaag atataataag tggctttgtt tgaccgaatt tatcaaacaa
    19861 taatcctcca aataaattac caatgagagc aaataaggca aatgtggagc ctactgttcc
    19921 tattagaata ggatttagat aatttctaag ataagttgga tattgtacag ataatgatga
    19981 aacatatata ccaacaaata taaatccaat agtcataatc caaaaatatt taattttttt
    20041 agcttctttt aatgtataac cccatactga aggagaaatt gtatcagatg ctttctcttt
    20101 tttaccttta gattttacct tttcattaat atctttaggc atcctgagta tgaataatat
    20161 tacaggtata ccaactatta atgctataat tccaaatatt agataagaac gactgtatcc
    20221 ttgagttgct aataagcgaa ctgcaagtgg ttgtagaaat atactaccaa tagaaccacc
    20281 agcaaaagca aagcccaaag cttttccttt gctttcttca tcaaaccaag cacttattaa
    20341 aacaggtatt cctatagcag atataattgt agcgccaatt tgaacaatac atgatacagt
    20401 gtaaaattgc catagttcag tacatataga aaaagtagca aaagcaatac tagaaattat
    20461 acaacctaat aaatatagta tcttaatatt gactttagaa aataatacac ctataaaagg
    20521 tgatgcaatt gctgaggcaa tagtaccaat agtgaatatt agagaaaaag aagctaaact
    20581 aaagccttct ccttctacaa cataacttat aaattgaggc tgtatacttg ctacaatact
    20641 aaatggaaca gcttgtatca acatacaacc aagtactata acccaagcat ttgaaaagtt
    20701 tttaatgcta aatttattgt ttagtaatgt attcatggta tctctccttt aggttatgat
    20761 ttgtagatat taatctaatt attctaattt tatatattag ttttggatag cccaactatt
    20821 taaatataat ttgggtatta atataaaaat taactataaa aattgattat tttaaatgta
    20881 tggggtaaat taaagtatat aaataaatga aaggatggat aaaatgtcat ttgaaaaaat
    20941 aaataataat aacctagata aaatacacaa caggaagtgt attatgcttg taaactttaa
    21001 taaaaaagaa ttatcattaa ttaaaaacgt aagtaacttt gctggaataa gagaccatat
    21061 catcttaagt gataaaaatg gagagaatat aataagagac attttagatg gaaatatatc
    21121 aaatgaatgt gaaaatggga ttaagagtaa gtctgtaata tttaataaca ctccaagcac
    21181 acaagtaaat gcatttttag aagggctaaa aaaagtgaga ataaatagac cttttacagc
    21241 tatggtaaca gagacatcta ttgattggac attgaataat ttaatagaaa atttagttca
    21301 agaacgtaat gctatgtctt taggaaaaac aattgaacat aagtaaattt aagatatctt
    21361 atagtaattt taactaatgt acattgagta tcatattata ttttagaata taaaatttta
    21421 aatgtgggta gatggaggag atttgagatg aaagttttat ttacaataaa ttatggaaaa
    21481 gaaaaatttg ataaaataag agaattaggc tatgatgtca tacattactc tgaagatagt
    21541 attgaaaata atgaggatgt aaatacagca gatgtactag ttacatacaa tccatttgag
    21601 agattagata tttctaagat gaaaaattta caatatatac agacaactag tgtaggtata
    21661 gaccaacttc caaaagaaga ggtattgaag agaaatatta agatagctaa taatagaggc
    21721 ggatatagtg tacctattgg tgaaagtatt gtattgtata ttttagaaat atataaaaat
    21781 agtgcaaaat tttttaaaca acaacaaaat aaagaatgga agatggattt ttctgtatca
    21841 gaattgtatg gacaaagagt tggattttta ggaacaggta ctatatcttc agaagcagca
    21901 aaaagattaa aagcttttgg tgtagaaata tggggtgtca atactgatgg aagtagtaaa
    21961 aaatactttg ataagtgttt tgctgctgag gatatgaata ttgtatttga agaatgtgat
    22021 atagtagttg tagcaatgcc ttctacaaaa agtacagatg gaatagtaaa caaagatatg
    22081 tttaaactta tgaaagatgg ttctgtattt ataaatgtag gtagaggaaa tacagttaat
    22141 cagaaagatt tagaagaatg tgtagagaaa tttagagggg tagcattaga cgtatttgaa
    22201 agtgaacctt taaacaagga caataagttg tgggagtgcg aaaatgtcat agtaactcct
    22261 cataattctt gggtgtcaga taaaaacaaa gagagaacat ttaatatggt ttataataat
    22321 ttgaaacact atataatgga gaataaacct gtagataatg ttgtagacat atcaagaggc
    22381 tattaaaaat aaaaaatttt gataaaaata aaaactctta ttagtcgtat taaagatact
    22441 aataagagtt ttttttgtaa taaatttaaa gccaaaaaag tgatatacta aaaaatatat
    22501 aaataggaga taattaatta tagggaaatt tcaaaataat ttaattttaa aaatatgaag
    22561 aggtgcaaaa agtgagcgta aaagatgaaa ttaataaact tatagagaca ataaactacc
    22621 ataatgaaag gtattataat caagatagcc cagaaatatc agattatgaa tacgataagt
    22681 taatgaagga gttaatttct ttagaagaag aacatccaga actaaagaaa attgattctc
    22741 caacaaatag ggtaggaggt aagccattag ataagttcaa tcaaattgta cataaaactc
    22801 ctatgttgag tttatcaaat gctttttcag agcaagattt gagagatttt gataagagag
    22861 ttcaagaata tgtgggagaa aatgttgaat atgttgttga atttaaaata gatggtcttt
    22921 ctgtaggtct aacttataac aatggagagt ttgaaaaagg agcaactaga ggagatggag
    22981 ttgtaggaga agatatttct caaaacttaa tgacagtaaa aagcatacct cttaagataa
    23041 atgataaaaa agaactaata gttagaggag aagtatatat atcaaaagat aattttagaa
    23101 aagtaaatga acaacaagaa gaacaagaac agccattatt tgctaatcca aggaatcttg
    23161 cagctggttc attgagacaa ttagacccaa aactaacagc aaagagacca ttagatatat
    23221 ttgtatttaa tatggaaaat atagaagaat acgatttaga aagtcacagt gaatcactag
    23281 agtacttaga aaaactagga ttttcagtaa gtcagagtta taaagtttgt aaaagtatag
    23341 atgaagttat tgagcatatt gaatactgga cagataacag gggtaattta gactacgaaa
    23401 tagatggtat ggtaataaaa gtaaacaata taaaacaaag agacgaaatg ggatatacag
    23461 ccaaaagtcc tagatgggca attgcatata aattcccagc agaaaagaag aaaactaaaa
    23521 tagtagatat aatagtagaa gttgggagaa ctggtacaat aacaccttca gcaattcttg
    23581 aaccagtcag acttgcaggt actactgtaa gtagagctac acttcacaat gaagattata
    23641 taagagaaaa agatataaaa ataaatgata cagtattagt tcaaaaagca ggggacataa
    23701 tacctcaagt tttagaagtt ataaaagaag aacgtactgg agatgaaata gattttaaaa
    23761 tgccagaaaa gtgtccagtt tgttcagagc caacaataag attagaagga gaggctgctg
    23821 taaaatgtat caatatgtct tgcccagccc aaataagaag gggaattata cactttgtgt
    23881 caagggatgc aatgaatata gatggattgg gagaatctat aattacactg ctattaaatg
    23941 aaaaaattat tcaagatgta tctgatttat attatataaa aaaggaagat gtagtcgacc
    24001 tagagagaat gggcgaaaaa tctgctgaca atttaataaa ttcaattgaa aaatcaaaag
    24061 aaaatgattt atggagactt ataaatggat taggtattaa atttatagga gttaaagcag
    24121 ctaagatact tgcatataac tttaaggatt tagatgaagt aatttctgca actagtgagc
    24181 aactagaaga attagaggaa tttggaagta taatggcaaa cagcgttgtc gagtttttta
    24241 aggaagacaa aaatatgaac gttataaaca agttaaaaaa tgtaggagta aatacaaaat
    24301 cattggataa tacaggtgaa aaagtagaaa aaatatttga agggatgaaa attgtattga
    24361 ctggtacact tcctacatta aaaagaaatg atgcaaaaga tatgatagaa aaaagaggtg
    24421 gaaaagctac atctagtgtt agtaaatcga caacatttgt tttggcaggt gaagaagctg
    24481 gttctaaact tactaaagca aatgatttag gaattaaagt tatagatgaa gagagatttt
    24541 tagaattatt aaagttatca tcgaaagatg aagtaaaagc tgtattagag agttaaaaag
    24601 tcgcttaatt tagtagaaaa ctactataat taatgataga atagttcagt aagctaataa
    24661 gattacgaaa aatgacaata attaatattt gacaaaatta aaaattataa aaataaattt
    24721 ataaaaaata aaaaatatga gaaaggatat aaaaaatgac cagaagttat gatagaagta
    24781 atgaacagat aagacctgta aagataacta gagattatac aaagtatgca gaaggttctg
    24841 ttcttataga aatgggagag acaaaggtta tatgcactgc atcagtggaa gaaaaagttc
    24901 ctcctttttt aagaaatagt ggtactggat ggataaatgc tgaatattct atgctaccaa
    24961 gagcaacaca acaaagaaag ataagagatg catctagggg aaaaattgat ggaagaacac
    25021 aagaaataca gaggttaata ggaagagcaa taaggtctgt tgtagacttg aataaattag
    25081 gagaaagaac tatttgggta gattgtgatg tgattcaggc agatggtgga actagaactg
    25141 catctataac aggggcattt gtagcagttg cagaagcaat atataaatta tataaagcaa
    25201 agacaataaa agagatgcca ataacaaatt ttgtatctgc aattagtgtt ggtattgtaa
    25261 atggaataca catgctagat ttatgttatg aagaagattc taaagcacag gtagatatga
    25321 atataataat gactgataag tgtgagtttg tagaagtaca ggggacagga gaagagaaac
    25381 ctttttcgag agcagattta aatttattgt tagagttagg tgaaaaaggg aataaagaat
    25441 taataaggct tcaaagagaa gctttaggtg aaatatcaga tgagatattg ggtatggatt
    25501 atggtaatga agtagtgatt gctactaata atgctcataa gctagaagaa ataggtgaaa
    25561 tcttaaaaga ttttgaatat aaagtgtact ctttaaaaga tgttgattta gcaggtatag
    25621 agatagtaga agatggaaaa acatttgagc ataatgcgtt gataaaagct agaacaatag
    25681 caaaaaagac taaacttata gcaatatcag atgattctgg cttagaagta gatgctttag
    25741 gcaaaaaacc tggtgtttat tcagctagat atgcaggaga acatgcaaca gatgaagaaa
    25801 atagaaaaaa actgttaaaa gctatgcaaa atgtaccaat gagcaaaaga aatgcaagat
    25861 tcgtctctgc aatagcagta gtttttcctg atggaaaaga atttgtagta agaggaattt
    25921 gtgaaggaat gataggattt gaagaaaaag gtaaaaatgg ttttggatat gatagcttat
    25981 ttatagtaaa aggatatgat aagacttttg gtgaaatacc tagtgttata aaaaattcaa
    26041 tcagtcatag agcaaacgca ctaaaactta tgaaacaaga gtttataaaa agggtagtga
    26101 agtaatgaaa ataggcatag taagcgacac acatatgatg attaaaaata tggaaaaaac
    26161 aataccatat ttgaaagaat gtgacctaat aattcatgca ggagataact ttactgattc
    26221 aagatatatt catagcatga ctaatgtagg tattattgca gttaaaggta attgtgactt
    26281 tgatgctgtt gaggaagaag ttgtctttga agtggcaaat aaaaccatat tcttatgcca
    26341 tggagataag tatggtgtaa aatatggaac taatatgtta gaaaaaaagg caactgaagt
    26401 tgatgctgat atagttatat ttggtcatac ccatacccca tttagagaaa ttaaagatgg
    26461 tgtactttac ataaatcctg gaagcacttc acttccaaga ggagtatcgt ataaaagttt
    26521 tgtcattatg gatatagaag aagatgacat aaaaattgaa gaaatacgca tataaaggtg
    26581 tatttatcta aaaaaatgga aaaaaacgct caaaaaatta aattttatca aataagtaca
    26641 atgatattaa tggaacactt gtaaataaaa aaacttatgt gatataatta agtggttgaa
    26701 ttataaatag aatgaattat aataccgtta tcgggtatta cataaatatc actaggagga
    26761 ttagaaggat gaaagcagaa ctaattaaaa aagagggtaa taaagttact ttaaaaataa
    26821 cagtagataa caataaattt gaagcagcag taaataaggc ttataataaa agcagaaata
    26881 aatacaatat acctggattt agaaaaggta aagctccaag aattgttata gagactcaat
    26941 atggaaaagg tatattctac aatgatgcta tagaaatatt attcccagaa gtttatccag
    27001 aagctatcaa ggaattagac atagacccaa tagataatcc agatttagat atagaagaaa
    27061 taagtaaaga caatgggttg gttatggttc ttaatgtaga agttaaacca gagtttgaat
    27121 taggaaacta caagggtata gaaatagcta aggttgagaa tactgtaagt gacgaaaatg
    27181 ttgatgctaa actacaagaa atggttgaaa aaaatgccag attagtaagt gtagaagaca
    27241 aagctttaga agatggagat acagctataa tagacttcga aggttttgaa aatggtgtag
    27301 cttttgatgg tggtaaaggt gaaaattata acttagttat aggttcaaac acatttatac
    27361 caggatttga agaacaatta gtaggtaaaa aagctggtga agaagtagaa gttaatgtta
    27421 ctttcccaga agaatatcac tcacaagatt tagctggaaa accagttgta tttaatgtaa
    27481 aaataaatga tgtaaaagta aaagaattat ctgcacttga tgatgaattt gctaaagaca
    27541 ctagtgaatt tgattcttta gatgaattaa aagcagatgt aagagctaag ttagaagaag
    27601 aagctaaaaa tagagcagat gcagaaacta gaaattcagt agtagaaaaa gttgctgaaa
    27661 atacagaaat agaaatacca gaagttatga tacaacatca aatagataat atgttaaatg
    27721 aacttaacta ccaattacaa tatcaaggat ttggattaca acaattacta gagatgactg
    27781 gtagaactat ggaagaatta agagaagaga gaaaagaaga tgctaaaaaa ttagttaagt
    27841 cttctttagt attagaagca ataactaaag ctgaaggaat tgaagcaaca gaagaagaat
    27901 ttaaggctga gttagaaaaa atggcttctg cttacaatat ggaagtagaa aaaatagaag
    27961 cttctttaag agatgccgac aaagaagata taaaaggtca aataaaaata agaaaaacaa
    28021 tagatttatt agttgataat gcaacaatag cttaataaaa taaacgctaa ctaatatagt
    28081 tgacgttatg aaatgtttag tatataatga tgtgcaaata taatttagtt aaatgcgtat
    28141 aataatttac agtagcttaa aaaaagctac tgtaaattat atagaataaa gggggtatga
    28201 aaccatggca ttagtacctg tagttgttga acaaacagga agaggagaaa gatcttatga
    28261 tattttttct agactattaa aagacagaat aatcttctta ggagatcaag ttaacgatgc
    28321 cacagcaggg cttatagtag cacagctatt atttttagaa gctgaagacc cagacaaaga
    28381 tatacattta tatataaatt ctccaggagg aagtataact tctggaatgg ctatatatga
    28441 tactatgcaa tacatcaaac cagatgtaag tactatatgt ataggtatgg ctgcttctat
    28501 gggagcattc ttgttggcag caggagcaaa aggaaaaaga ctagcactac caaatagtga
    28561 aataatgata catcaaccat taggtggagc tcaaggtcaa gcaacagata tagaaattca
    28621 tgcaaagaga attttaaaaa taaaagaaac tttaaatgag atattatctg aaagaactgg
    28681 tcagccatta gaaaaaatta aaatggatac tgaacgtgat aacttcatga gtgcactaga
    28741 agccaaagaa tatggtttaa tagatgaagt atttacaaag agaccgtaga tagagagagg
    28801 tgttaatatg tctaaatatg aagaaaaacg acaattaaag tgttcattct gcggtaaaaa
    28861 tcaagatcaa gtaagaagat taattgcagg tccaaatgta tatatttgtg acgagtgtgt
    28921 tgaactttgt gacgaaatta ttcaagaaga gatagaagat actatagatg aggatactac
    28981 atctttacca aaaccaaagg aaatgatgga gattctaaat gattatgtaa taggtcaaga
    29041 aaaagctaaa aaagctttat cagttgcggt ttataatcat tataagagaa tatacagtaa
    29101 aaaatctagt agtaaggata tagaaattca aaagagtaat atacttttat taggacctac
    29161 aggttctgga aagacattac ttgctcaaac tctagcaaga actctaaatg taccttttgc
    29221 aatggcagat gctacatcat tgactgaagc tggatatgtt ggagaagacg ttgaaaatat
    29281 acttttaaaa cttattcaag ctgctgattt tgatatagaa aaagctgaaa gaggtataat
    29341 atatatagat gagatagata agattgcaag aaaatctgaa aacccatcta taactagaga
    29401 tgttagtgga gaaggtgttc aacaagcact tctaaaaata ttagaaggta ctgttgcaaa
    29461 tgttccacca caaggaggaa gaaaacatcc tcatcaagaa tttttaaaga tagatactac
    29521 taatgtatta tttatattag gtggagcttt tgatggatta gagaagataa ttcaaaaacg
    29581 tggtggagat aaaacactag gttttggagc taaaatagaa agtaaaaaag aattagattt
    29641 agggaagcta tatgaaaaag tacttccaga ggatttatta aaatatggta taataccaga
    29701 gtttatagga agaataccag ttttagctac tttagagttg ttggatgaag atgcattaat
    29761 gcagatatta caggaaccta aaaatgctct agtaaaacag tataaaaagt tattggagtt
    29821 agatgatgtt gagctagaat ttgaagaagg tgctcttcgt gcaatcgcta aaaaggccat
    29881 agaaagaaat acaggagcta gaggacttag aagtatagtt gaaagcgtaa tgatggaaac
    29941 tatgtttgaa gtaccatcta gagataatat aaagaaggtt atagtaacag agaaatctgt
    30001 gaacgaagac tcagtaaatc ctatcatagt acttaaggat caagaggaaa gtgcataaga
    30061 tgatgaaaag gagctttaaa agctcctttt tttatgttaa tatgctaaat ataaaatagg
    30121 tatcattatt ataaaaatgt gagtaatata cctagaatac caaagacgaa tacaattgca
    30181 atggaaccaa tttttccttt atataaaaga taaaatgatg ctataaatgc aattatacca
    30241 tataagtccc aattaacttc tttggcagat gaaaaagctg ctgataatat gaatcctaga
    30301 gtaattggtc taagaactgt caataagttt tctacctttt tattattttt aaatttagaa
    30361 aataactttg atataatagt tagacctatt aatgcaaaaa ttgtaacacc aatagttgca
    30421 gctagagaac caaagaagcc agcagtctta aatcctacaa atgtagcact atttattgct
    30481 acaggacctg gtgtagattg tgatatagca agtaaatcca aaaactcttg attagttata
    30541 aagtgatatt tagatataaa ttcttgctgg ataaatggta gcatggcata accgccacca
    30601 aaactaaatg cccctatttt aaggaatatt aagaagagta gtattatttc tgacatagac
    30661 tatatacacc tcctccaatt attaaataaa tagggcttat attgaataca ccaacagata
    30721 aaactgctat aattaatagt agtatgttag ttttagactt tggtagtttc ttgaacatgc
    30781 tcgtaaaaga atacaatata agagctatta caacaggcgc aactccttta aaaaatccat
    30841 ctaaaatttt agatgatgaa tttttaaagt atagatacga taagactaag actatacaaa
    30901 aggatggtaa agcagagcct aatgtacata taagaactcc tggtatacca taaagtttat
    30961 atcctaataa tacagatata ttaactgcca gtactccagg ataactttga gctatggata
    31021 aataatcaat aaattcttcc tgctctaaaa gtttttgttt atttacaagt tcatcttgta
    31081 taagaggaac catagcgtat ccaccaccaa atgtaaacgc accaattttt aaaaaggtta
    31141 aaaacaattt caacataata gaaacctcca aaagataaat aacatctaag tatactaata
    31201 ttaatattat atcataataa aaagataata tagtatgata gtattctaag aatttatcat
    31261 taatgttgaa aattaacttg ttcaaatata taatattaaa agcaaagcaa ttagactaga
    31321 aaaggagtaa tgttgatgga acaaaattat actaaaatag atcatgaatt accattaatt
    31381 cctctaagag gattagctat atttccatat atgatattaa attttgatat aggtagagag
    31441 atatctctta aagctttaga ccaagctatg atggatgaag aacttatatt tttaacttct
    31501 cagaaagaag cagaggtaga tgagccagga gaagaggatt tttatcatgt gggaacaatc
    31561 tgtaaagtta agcaaatgat aaaattacca ggagatacag ttcgtgtttt ggttgaaggt
    31621 gtatcaaggg gtagagtaaa gaaaatagaa caagaagatg ggtatttcag agcagtaata
    31681 gaagaaatag tatttgatag tgataatttg gatagtgaaa cagaagttga aattgaagca
    31741 tttgtaagaa atgtatttga tgcctttgaa gagtatataa atattggaaa tagggtatca
    31801 ccagaaatat taatatcttt agctgatata gaagatgtag atagatttat agatacgata
    31861 gctgcaaaca tatatctaaa atcaagccaa aaacaagaaa tattagaaga atttgatatt
    31921 agaaaaagac ttgaacttat ttattctata ttattagagg aaatagatat attaaaaata
    31981 gaaaagaaaa taactttaag agtaaaaaaa cagatgaata aggttcaaaa agaatattat
    32041 cttagagaac aattaaaagc tatacagaaa gaattaggtg aagaagaaga tataaactct
    32101 gaagcagatg agtatagaga aaagcttaaa aaaataaaag cacctaagac aactaaagag
    32161 aaaatagaaa aagagataga taaattttca aaaatatctt ctatgtctcc agatgtatct
    32221 gtaagtagaa actatttaga tactatattc tcattgccat ggaataaaga gacaaaagat
    32281 aaattagata taactaaggc aaaagatata ttagatgaag accattatgg attagaaaaa
    32341 gttaaggaga gaatactaga gtatctagct ataagaactc ttgctaagtc acttaaaggg
    32401 cctataatat gtctagttgg tcctccagga acaggaaaga catctatagt aaaatcaatc
    32461 gccagagcat taaatagaaa atttgtaaga atatctttgg gtggagttag agatgaagca
    32521 gaaataagag gtcatagaag aacttatgta ggttcaattc caggaagaat aattaatggg
    32581 gtaaaagaag cccaaacaaa aaatcctgta ttcttatttg atgagataga taagatggca
    32641 gcagattata aaggtgaccc agcttcagca atgctagaag tattagaccc agagcaaaat
    32701 aaggattttg tggaccatta cttagaaata ccttttgatt tgtcaaaaat actatttgtt
    32761 acaacagcaa atagtctaag taatatacca agaccattat tagatagaat ggaagtaata
    32821 gaagtatctg gatatataga agaagaaaaa ttaaatatag caaaaaaata cctacttcca
    32881 aaacaaatta aagaacatgc tttaaaagaa aactttataa aaatagatga tgaaacttta
    32941 agaagtataa tcaaccatta tacaagagaa gctggtgtaa gaacactaga aagaactatt
    33001 ggtaagattt gtagaaaggt tgccaaaaaa tacgttgaag acccaactct tgaagaggtt
    33061 gttataaata agtctgacct agagacatat cttggaaaag acatgtttaa gtatcaactt
    33121 gcagaagtta accctcaaat aggattagta aatggtcttg catggacaga agttggtgga
    33181 gttacacttg aagtagaagt aaacgtactt aaaggtaaag gagaaattgt attaactgga
    33241 aaattaggag atgttatgaa ggaatctgcg aagacaggta tctcttatat aaggtcaata
    33301 gtggacaagt ttgatataga ccctgaattt tataagacga atgatattca tatacacatt
    33361 ccagaaggtg ctgttccaaa agatggacca tcagctggta taactatggc tcttgcagta
    33421 atctcagcac ttactaaaag acctgtgcct ggaaatatag caatgacagg agagataact
    33481 ttaagaggta gagtcttagc agtaggagga gttaaagaaa agttattagc agctcataga
    33541 gctggtataa caaaagtatt aattcctaaa gaatgtgaag ctgatttaga tgaaatacca
    33601 gaaaatgtaa aagaaaaaat ggagtttgtg cttgtagaac atatggatga agtattagaa
    33661 caagctttat taaagagtgg tgaaaataat gaaaattaga agttcagaga taactatgag
    33721 tgctgtgaat aaatcacagt atccagcaga aggaatccca gagatagcat tggctggtag
    33781 gtcaaatgtt ggaaaatcat caataataaa tacattgctt aacagaagaa acttcgctag
    33841 aaccagtcaa acaccaggaa aaactagaac tataaatttt tatctaataa ataatgaatt
    33901 ttattttgta gaccttccag gctatggata tgcaaagata gctaagtctg aaaaagaaaa
    33961 gtggggaggt ataatggaaa gatatctaga aagtagacaa gagttatgtt ctatattcct
    34021 acttgtagat attagacatg agcctacagc agatgataag cttatgtatg aatggataaa
    34081 gcactttgga tataactgtg tagttattgc aactaaggca gataaaatat ctagaggaca
    34141 atatcaaaaa cacataagta taataagaaa aaaattgcaa atggaaagta gtgaaaaagt
    34201 aattcctgtt tcttcactaa aaaagactgg tgttgaagaa ttatgggaag agattgtaaa
    34261 tcaatacaat caacatggat atgaaataac agtagattaa atttaaagtg ttaaatattg
    34321 acataagatt tatttgaata ataaatgtaa taatagttag ttttaattat attaatatta
    34381 aagcaaaata taggatgcct taaaatagtt ttaatgaact ttttttggca tccttattgt
    34441 ttataaacaa taaatatcag taattttgct aaatactaat aaattttagt aaggtatggg
    34501 aaaattagta taaattagtt caactaaaaa attattgact tatgtagtca gcaataaata
    34561 gcatttaaga aatagattta tctgtttttc tcataaagaa aacagttagt ataagcccta
    34621 taaaacatac tgatgctgca atccaataag actgattgct agctataaca taggctgatg
    34681 tgtgactttt aaacttttga ttgtatgtaa gactttgtgc aaaaaaacta ctaaaaaatg
    34741 ctgttccaag agcaaatgat aaattacgaa tagttgcaag aaaactgcct ccgtatccct
    34801 gtttttgagg aggaacatct gctaaaatac tactattgtt tggtgaacta aatgctccta
    34861 gcccaagtcc aaaaataact aaacatacaa ttaaaagata caatggagaa tctgctttta
    34921 aaagtgccat tccaactagt gagatggtca taaatgaaaa agatgctgtt agaatgtttt
    34981 ttgcaccgtg tttatcagaa agtgaacctg caataggtgc tataagtacc ataataagtg
    35041 gatgtacagt cattacataa ccagccatca taggagataa attttttaat tgttctaaat
    35101 aaaatggaag tagaaaagat actgccattt gaggaaaata acaagctact ccaatgatat
    35161 tgcctacagt aaagtttcta tttttaaata gaggtaggtc aataaagctt tgttcaaatt
    35221 ttacttctct taatgccaac aataagaaga ttataagtgc tgttatacct aaatacaatc
    35281 ttgtattacc tttctcattc attgcaagta atataagcgt agtgcaagat gcgaaaagta
    35341 gaagacctgg aatatcaaag ctcttgtttt gttcatccaa aacaggttta ggcagaaatt
    35401 taatgccaag aaagactgct ataataccaa taggtatatt gataataaaa atagaaggcc
    35461 atccaaaatg ttctaaaatt atacctccaa ttacaggacc actcatattt cctataccaa
    35521 ctacagctcc agtgattcct atagcttttc ctttttcatt tgctggaaat gcattggaaa
    35581 ctataccaag cccagtagcc attaaaatac ttgctccaac tgcctgaaag attcttgaag
    35641 aaattaaagt tgataaattg tttgacatac tacacagaaa agaacctatt gtaaatataa
    35701 aaaatcctaa tgtgtataga cgatttgaac ctactttatc gccaagtttt ccgaaaaata
    35761 acatagtagc tgttacaaca agcatataag ctgttacaac ccactgaatt tgtgacatgt
    35821 taattttaaa gtcagcagca attgtaggat ttgcgatatt tagtatactt ccatcaagag
    35881 tagacataaa tacaccaata cagatgataa tcaatactat ccatttttgt ttcatgataa
    35941 cactccaatc ttaatagatt ttataagtac gatataattg taaaataaac aacaaaaaaa
    36001 gcacctgtat attaggtacc ttgaatgtgt ctaatttcat gaataaatac ggtagttgaa
    36061 ggatttgtat agattactca tgtaaaatta cataaaggta ctcatatact attttcttct
    36121 accatccaga ttttactgtc ggctttggaa tatgaccaaa ttatgccata aggctcgcgg
    36181 gcttttaccg ccgataggga atttcacccg tttatgaaga aaaacagatg tattttgtaa
    36241 tattatttta acataaaatt ataatggaaa caattggatt taagtaatat ccagctgtat
    36301 tacaatacaa atatttcaat atggacactt aaattgtatt tatagataga ataaggaaca
    36361 gtatgagaat ctataataat tagataaaat taacctgtta ttagtattag atggtaagtt
    36421 ttaaatttat caaaatcaga aagctgtaat aaaattctta taatatgaat atcttaatta
    36481 agagatattg taaattatga ggaggcgcta atatggaagg aaaggtaaag aaggcattcc
    36541 cgggtggaaa tacatcacaa ggctcatatt ctttttttga ctatataatt cctgaaaatg
    36601 taaaaagagt tttttgttta aagggtggac ctggagtagg aaaatcctca cttatgaaaa
    36661 aaatttataa agagtttttg catagaggat atgatattga tttatatcat tgtccttctg
    36721 acccaagttc gttggatggt ttagttataa agaaattagg cgtggtactt atggatgcaa
    36781 ctgccccaca tactatggac ccaaagctac ctggagcttt ggatgagata attaatttag
    36841 gtgattactg gaatacagaa gaattagaaa agaataaaca agaaatatca gtttatgata
    36901 aagatattag taattcattt aaaagagcat ttaagttttt aaaatcagca gaaccaatct
    36961 ttagagacat agaagaaaaa tattctgatt gtatggatta tggaaaagta aatttggtca
    37021 cagaagaatt tataaaaagg ttatttgatg gagttaaaag cacaggaaat ctaaaaagag
    37081 aaaagcattt atttggttca gctataactc ctgtagggtg tttagattat acagaaaaca
    37141 ttttaaaaga tgtaaagaaa gtctattatt tagatggaga aataggaact gggaaaacta
    37201 cattattaaa caaagtatat aagaaagcta ttgaaaaagg tttaaatgta gaagtttatc
    37261 attatccact tattccagag aaaattgaaa ctgtactatt aactgattta gatataggca
    37321 taactataag tacgactttt aaagaagaag atacaattga tttaaatcag tttttaaata
    37381 gagaaaaact acttaaatat gaagaagata ttaaatttga tgaaaaagtt ttagatgatt
    37441 taatttgttg ggcagtacta aatcttaaga gagccaagtc taaacatgat attatagaaa
    37501 gttactatag tcctaatatg agatttgatg aagtagggaa attaagagat aagttaatta
    37561 aaagaatttt aaaatatgag gaaaacttat aaacgcatat gtttaaaatt aacgttagta
    37621 ataatataat ttttagtaaa ttggtgtagc tttgaggcta tgccatttta ctttttttgc
    37681 taaaaaatga tattaataat ctccaaagcc attatttgtt tttcataatt attatttcct
    37741 ttcctaattt atgttttttt ataatatcac aacaatgttt attgaaagtt tatataattt
    37801 taagaacatc taaaaatgca tacttgatat tggtaaatta gtgataggag ttcaatgtat
    37861 gatattatat actaaagatg aattttaata tataactttg atataataag catatgtaaa
    37921 tgtttaatat ttaattaaag aaaattatca tataaaaata aatttgtaag gagcaataca
    37981 gtatgaaaaa acaatatgtg tttataatac tagcaagtac tattttaggt atacttgttg
    38041 gaaccatatt tagagattat agcaatgaag aaacagttta tacaagcaaa gacaagtcaa
    38101 taagaaaaga aataaaaatg acaagaaaat ctataaaaga attaaccaaa gagaaagaca
    38161 agttagataa agaaattaat tcaatgaaac aaaatcaaaa aaattctgaa gaaatatcag
    38221 aaatagaaac acttagagat agtctatcat attcagatgt aaagggtagt gggataatat
    38281 taagtataga tgctatagat gaaaaaacag ggaatatagc taatagtata gactataata
    38341 agattcttgt aaaagttata aatgaattaa aagtaaacgg aggaaaatac atagaaataa
    38401 atggtcaaag aattaatcaa tattcagaag ttgtacttgc aggaagccat ataaatataa
    38461 atggagttcc tgtagcacaa ccatatgaaa taaagtgcat tggagataag gagaaactat
    38521 ctaattatgt agataaaggt agtaaatatt tagatagcat acaaaccaat tatccaatga
    38581 aaataagcat aaaaactgaa gaaaatatct ctatgaaaaa aatgaatata cctaataaat
    38641 taaagtatat aaagggtgat taagattata ttagaggaaa gcagtttgcc aatgttaata
    38701 ccagaaaaac ttgcaaaaaa atataatgta tttccagttg agtttcaaga tgataattta
    38761 gttgtagaaa tagaagatga ggatatttat gctctacagg acttgagatt agctacaggt
    38821 aaagaaatta tattgaagaa ggagaaaaaa gaacttataa gtgaaaagat tgaaaaatat
    38881 tataatagct ttgaacttga tgaggattat gcaaaaaaat tatttgaaaa tcttttagaa
    38941 aaagctataa aagaaaacgc aagtgatata catatagaac catttaaaga acatttaata
    39001 gtgagaatga gggtagatgg agaactaaag gaagtatcaa agtttttagt tgatgtctat
    39061 ccatcattgt ctacggttat aaaattaaag gcatcaatgg atataacaga aaaaaggctt
    39121 cctcaggatg gtagggtgga tataaagtta ggtggaaatt taattgacat tagagtttca
    39181 tcaataccaa ctgtctatgg tgaaaaaata gttttgagga tacttaatag agatactttt
    39241 ttaaaagaca aattagaact tggtttttca gaggaagcta taaaatctat aaatcgtata
    39301 ataaataaaa gagcaggaat attattagtg acaggtccta caggtagtgg aaagactaca
    39361 acagtatatt ccttattaaa tgatttaaaa ggaataaata aaaatattat gactatagaa
    39421 aatcctgtag agtataagat ggatggcata agtcagattc aagtaaattc aaaagtaggg
    39481 ttgagctttg atgtaggtct tagagcgata ttaagacaag acccagatat aataatggtc
    39541 ggagaaataa gagatgcaga aacagctaag atagcagtaa gagcagctat aacaggtcat
    39601 ttagttataa gtactttaca tactaatgat gcagtatctt ccatagctag attactagaa
    39661 atgcaaatac caccatatct tttaaatgcc tctcttatag gtgttatatc tcaaaagttg
    39721 gttagaaaag tctgcaatca ttgtagccat gagattatga taaaagataa ttttagtgga
    39781 gatgtaaata ctaaggtagc tgtagggtgt gaaaaatgta atgataaagg atactttgga
    39841 agaacagcaa tttatgaaat cttagaaatt aatgatgata taaagacatg tataagaaat
    39901 atggaggatt ctagtactat taaagatgtg gcacaaaata atggaatgat aacatttgaa
    39961 gatagttgta aaagactaat aaatgaaaaa ataacgacat tagaagagtg tttagtagta
    40021 aatgaaatga tttaactgaa ttgtatagtt aaacttcgtt tggtcagttg tctttattta
    40081 atctatttta attaagatgt ttgagtataa attatattta agtgaaataa aattaaatgt
    40141 attaaattag aaaatattaa gtttatttaa ttgtaattaa tataatttgg aaggtattat
    40201 agtacatgag attatctaaa aatatatttt ataatttaga aaagttttat ttagtattaa
    40261 atagaatcag tgataaagaa attagagtaa tttgcaaaga gataggcata ttgctggaat
    40321 ctggatgtga aattacaaaa atatttgaaa tcattgagag tcagtcaagt aaaaaagtaa
    40381 agaacttgct tagtatagta tcaaatcata ttcaaaaagg taactcaata gcagaatcat
    40441 ttcaaataac aggtatattt tctaaattct ttataagtat gataaaagca ggagaaacta
    40501 gtggaaactt agatataata atgagtgatt tatctaatta ttatgataaa gaatacaaat
    40561 taaaaatgaa aataataact atttcaatat atccaataat acttataata ttatctatct
    40621 tatcaatgtt gtttatattc gtatttgtaa tacctaattt tcaggtggtt tttaccaaca
    40681 atggaattga accaccttta ataactagag ttttaatggg aatatctaca gtggttacaa
    40741 ataacttagc ttatataata tttagtttta tattgttttc aataggaagc atttatttct
    40801 ttataacaaa tgataatata aaaaaattga ttaattcgtt aaaattcaaa atacctttta
    40861 taaaaaaaat taatcagtta gtagctacaa caagattttc aagggcattg tccgttttaa
    40921 ttagcagtgg aatacaaatt gtggaagcta tagatatgtc agctagtgtt atagacaacg
    40981 aatttattta tgaaaggtta aagatttcca ataattatat aaaaaaagga aatagcatag
    41041 gtaaatcttt gaatttagca aatatttttc caaaattatt tatttcaatg gcaaatacag
    41101 gagaagaaag tgggaaacta gataaaagtc taaatgtaat aaataaattt tatgaaaatg
    41161 aattaaataa caaaatagag caaataatga aatttataga acctatgcta attgtattta
    41221 tgggattaat tattggtact tttattttag caatggttgt tcctatgttt gatgcaatta
    41281 ctaattttta gagagaaaaa taaattttat tagatgaaac ttttgaaaag gagagaatat
    41341 ttaattgata aatttgataa ataaaaaacg aaaaggattt acacttgttg aaatgattgt
    41401 agtagtaact attttaggcg ttatatctag tatagcatta gttaagtata gtaaggttca
    41461 agaaagtgcc aaattaaatg cagactatac gaatgctgct aatatagtaa ccgcagctag
    41521 catggcaatt aatgatgatg aaaagacaat agactctcta agtgtagaga cattgaagga
    41581 aaagggatac ctaaatactg ttccagttcc tcagagtaca tcaggtaaat tcgaacttgt
    41641 cataaatgat agcggaacag atataagcgt aaatataaat tctaagcaat tttatccaaa
    41701 ataatatttt atattgattg ctataagttt taaaattatt tttatttaag aaggagatgg
    41761 ctataattaa aaaaattcta aacttagaaa taaatgatga ttatataagg cttgtatttt
    41821 taagaaaagg tcataaaaaa gtttttatag ataaatcaat tataaaagag attaactttg
    41881 atataaaaaa tgatagtaat tcgattgaga agattaaata tatagaagat gtagttagat
    41941 ttgtaaaaga ggagattggt aaatcaaaaa tattatataa aaatttatac tttaacattc
    42001 aaattcagga tatagtcatt agaaatgttg aagttctaaa tactaagaaa aaaagagaca
    42061 tattaagcat gattgaattt gaaataacac aatacattcc aataaacata aataactatt
    42121 caataaaata taaagttata aattctaaaa atgataaatt gaatatacaa gtaattcttt
    42181 tcccaaagag tattattcaa ttaattaaga acatttcaga aaatctaaat atgaatccaa
    42241 agactataaa tataaatttt gatatattac aaaagcttat aaacttaaat ttaatagaga
    42301 atttttcaga agaaggggta tttatagaat gtaaaaaaag tcagttaatt ataaatataa
    42361 caaaagataa aaaaataaga gaaacatact tgctatcaaa agagggaaaa tcatatgagt
    42421 tcataaaaag attattaagg gattttaagt atgtttatta ctatggtata gaaaattgtt
    42481 ttattgaaga atactttaaa tatacagata aatctattga agtaaatcca ttaagattaa
    42541 aagataaagt aaaagtagaa aataattttg atgcagattt agaagacaat aggataaatt
    42601 atataaatag tataggaatg attatttaag tgaaagtgga taaaacaata aattttctta
    42661 atggatttaa aaaaactaaa ccatgtaata aaatctataa aataataata gtcttaattt
    42721 taacgtgtat tttatttaaa tcatttatgt atgtgaaatc tataaatgaa cttgaagatt
    42781 atataagtca aaataagcta gtggataaat caagacacac tttaccaaca acagataaaa
    42841 ctagagagaa attttctaaa ataaaactta atgaaataaa aaaaatatat gaaacaattg
    42901 gaataaatag tattactaaa atatcaatag ataaaaataa gctagagatg gaaggtgtgt
    42961 gtagtgattt agatgtttta aacaatttaa ggaaaataaa gtacataaaa tattattaca
    43021 tagacagtat tgtaagagat ggagaatatt atagatttag aataaaaagt tagatgggtg
    43081 attatttttg agattaaata atagagaaaa aaatctactt aaaatattgt taatattagt
    43141 tattatattg gcaagtgata tagcagtaag ttatttggaa gaaaacctaa ctagtttgaa
    43201 gaatgaaaag caaaatataa taaataaaat aaataatcaa gataattatc aaataaagga
    43261 taataagcat atagatgata aatatataga aagtgttgaa tcaaataaaa atgaagaatt
    43321 aaaaaataat tttaataact catttttaga taaaaaaaca aataaagata tagtgttatt
    43381 agtagaaaat gaactgagag atattgtgaa tgtagaatat attaatcaag aaagtagttt
    43441 agataatcac aataaaaaac aagttaattt agaaataaaa gttaatggag aacttgcaaa
    43501 tgtgttgaaa cttgaaggtg taatgaagaa gttaagacta aataataaac ttgaaaagat
    43561 agaaattaat aaattaaaag tcaataattt tgatgttcaa aatattccaa atgaatataa
    43621 acaagaaaaa gacaatattg ttgaatgtaa aatgacagtt agagtcatat aacaaaaaag
    43681 aatagataaa gtaaaaaact attatcaatt aataaaattt tatagaggaa ttagttttac
    43741 gataattcat ttctacaaga gggattgttg atgattttat cgaaatccta attaaagtta
    43801 atatattgta ccaaaatata agtgaattta atagaatatt tacttatact atagattgat
    43861 taataaaaaa atggtataat aacaaattgt actttaattt aataataagg gagtggaagt
    43921 catattgtta gggagaaatg agctatgccc ttgtggaagt ggcaaaaaat acaagaaatg
    43981 ttgtttaaat aaagatattg tttcagaaag agcatgcaga aaaactgaac tttcacaaaa
    44041 acaatataca gatttatatt caaagttata tgattattca agacaggata agtttaaaga
    44101 agaatgtgaa aaagctaaag aaatgtttta tataatgcaa aatgatagtg taaatgaaaa
    44161 atttgaaaga ttttttaata cttatttaat acaagaccac ataatggaaa ataaaaaggt
    44221 tatgacagtt ggttttcttg aagaaaatgg tgcaaaccta agtcaatcag aagtatcaat
    44281 attaaaaagt atgtttgaat catacgtaag tatttatgag gtaaaagaag tttctgctgg
    44341 aaaaatagta ttaaaggact gtttaagtgg aaatgaactt tgtacagaag atgtaaaact
    44401 tctgagaagt tttaaaatcg gaagttgtat gatagctaga atagttgata ttgaaggaac
    44461 taacatattg atagatataa ctataagtat atcaaatgag gttaaagata taattttaaa
    44521 ggatattatg aatctattca atcaatatca agatgtatac aaagatatga agtcgttctt
    44581 aatttatcat acacatatac tatacaagta tatacaacag cttttagacc caagtatagc
    44641 tgaatattta aagagagaaa gagataaaaa cgatacaaaa gttaattcag aaaatgaatt
    44701 agctgaagaa gaatgtaaag ttttagatac tcttaaagaa aatgtagaaa aagaaaatat
    44761 agatgcatgt atagagtttt ggaatgaata taagaaaaat catgaaaata taaaaggagc
    44821 tgaaaatggt tgggcagctg ctgttgaata ctatgttaaa aaagaagcag gacaacctat
    44881 aactcaagtt caaatatcaa agaaatacga tataagctct agtacactag gaaagagata
    44941 caaggattta aaaatatctt aattaattat aaaaagcttt aagtcataga taaagaatct
    45001 atagtataat atatgtataa tgtttagaaa gaggtattaa taatgagtct tagtttagga
    45061 aatgcaattg ctgtatcaac gaactttata aaatctaacc cagctgtacc tgtgtacaac
    45121 ggagaagaaa tatatgcagt tatttatcat atagaaatag gatataataa tggtgtatca
    45181 cctgatacca aacaatatat aataatagac tataaagagt ttgatttatc tatggataaa
    45241 aaaaatgaac taataaatat tgctaaagag tattgtaaat ctaaaggtat tgtagaaaac
    45301 gaaaaccttg aaataaacct tttagacaat gaagaagcag agaacttctt agaaaaggtg
    45361 tttaataata tgaaggttat aagaggtctt ataactaaaa aaggataaat aaatggacta
    45421 tataggaata gaaaatataa caccttatga aaatacatat gaatttagtg tatatgaata
    45481 tgatgatgaa atcaccttag gtagtgaaaa gttatatgta tgtgaattaa gggttgtatt
    45541 gattaaagtt aattctctgt atgttgaaag attgcataaa tcagttgaag caatggtctt
    45601 agtaaaaaac ttgaaaaaag atttagataa aacacttgtt gtaaacaaaa taaagaattt
    45661 tgtgctagat gagatttggg tagaaaatct agtaaaagag aatatagaag ttatatttgt
    45721 agaaagctag gaataaccct agctttttta tattacaatc taagagagga aaacaatatg
    45781 ttaagagtat ctaatgtaaa acttggaatt gataaagata tatctttatt aaaagatgtt
    45841 atacttaaga agctaaaaat aaaagaaaaa aacttagtca aatatagtat atataaagaa
    45901 tctatagatg ctagaaaaaa aggaaaaatg gaatttgttt attctgtaga tgtagaagtt
    45961 aaagatgaaa ataaaatact taactcaaaa ataaaagatg taacaaaaat aaaggaaatc
    46021 agatacgtaa atgtacctgt gggtgataaa aaattaaaga ataatccttt ggtaattggt
    46081 agtggaccag ctggtttgtt tgcaggtctg cttttagctc aaatgggtta tgaaccaatc
    46141 atacttgaaa gaggtcttga tgtagacagt agaaccaaag atataagtga tttttggaca
    46201 actagaaaat taaaaaataa ttcaaacgtt caatttggag aaggtggagc aggaactttt
    46261 tcggatggta aacttacaac tagaataaaa gatatacgat gtagaaaagt attagaagag
    46321 ttagttcagt ttggagcacc agatgaaata ttatactcac ataagccaca tgttggaaca
    46381 gatatattaa aaaatgtagt taaaaatata agaaatgaaa taataagttt aggtggtcaa
    46441 gttagattta attcaaaagt aacagatata gttgtatcaa atgattctat acaatctgta
    46501 ataataaatg atactgaaaa aatagataca gatacaatta tattagctgt tggacatagt
    46561 gcaagagata cttatgaaat gttgtattca agaggtataa aaatagttca aaaacctttt
    46621 gcaataggag ctagagttga acatccacaa gatttaataa ataaagcaca gtacaaggat
    46681 ttttataatc atcctaggtt aggagctgca gattatagac ttatagagca tgtttcaaat
    46741 ggaagaacag catatacttt ttgtatgtgt ccaggaggta gtgtcatagc atctgcatca
    46801 aatgattttg aaatagttac aaatggtatg agtgaacatg caagagataa aataaatgca
    46861 aatagtgcat ttttagtaaa tgtaacgcca gaagatttta agagtgataa tccactagca
    46921 ggtgttcatt tccagcagaa gtatgaaaaa ttagcttttg aattaggtgg aaaaaattat
    46981 aatgcaccag ttcagcttgt tggagatttt ataaacaata agacttcttt gaatttagga
    47041 agtgttgagc catcatatag accaggatat acacttacaa atttaagtga atgtttgcct
    47101 agcttcgtaa cagagactat gagagaagcg tttatatctc ttgataaaaa attgaatggg
    47161 tttgcaatgt atgatgctgt tttgacggga gtagaaacta ggtcatcatc accaataaga
    47221 atagtaagag atgaaaatac tttagaatca gtgagtatta agaatctata tccatgtggt
    47281 gaaggtgctg gatatgcagg tggtatagtt acagcagcag ttgatggtat aaaatgtgca
    47341 gaaaaaataa tacaaaaata caccaataat acaaaaattt aacataaact taatgatttt
    47401 ttaatattta acatgaattt aacattatca ctcctataat ttgataaaat acaataggtt
    47461 ataaaatttt tagatacagt aatctcttta caaggaggaa ataatttgaa cattataata
    47521 agtctaatgg gtggattagg tctgttctta tatggtatga accttatggg agaaggtctt
    47581 caaaaatctg ctgggacaaa gctaaaaaag ataataaaac tacttactag taacctattt
    47641 atgggagttt tggtaggtac aggagttaca gcagtaatac aaagttcaag tgctactact
    47701 gtaatggttg ttggtttcgt aaatgcagga ataatgactt taaaacaggc aataggagtt
    47761 ataatgggtg ctaatatagg gactactgtt acagcccaat tggtatcttt cgacttaact
    47821 ggtatggccc cagtagcatt aggtgtaggt attatacttt atttatttgg aaataagcct
    47881 agaataaaaa atatagccga aatacttata ggttttggta tattatttac aggtatggat
    47941 tttatgaaga tggcagtgga gccactaaga gactatcaag gatttactga cttacttgtt
    48001 acatttggta gatatccact acttggatta ttattgggat ttgggattac agcaataata
    48061 caaagttcaa gtgcatctat gggtatgttg gttgcactgg cagcagaagg tctagtgcca
    48121 ttatcagcag cactgccaat tctttatggc caaaatattg gtacatgtgt aacttcactg
    48181 ttatcaagta ttggtgctaa caaaaatgca cgaagagcag ctatgatgca tctaatcttc
    48241 aatgttttag gaacagtgat attcttaata ttcttgaata aaccagtagt aagcatggtt
    48301 acaagttggg acccaagcaa tgttgcaaga caaatagcta atacacatac attgtttaat
    48361 ataatatctg tattgatact tttaccattt acaaatctta ttataaaact agctataaag
    48421 cttgttcctg atagagcagg tgatatagat gaagatgaaa ctaaaactat taaatatata
    48481 gatgatagaa tgatagaaac tccttctata gctcttgcaa atacgataaa agaagcactt
    48541 cgtatgggtg agaaggcgaa ggaaagccta aatgcatcta tggaagcttt agtagaacat
    48601 tcaactgaaa aaatagataa gacatatagg cgagagagat taatcaatga tttacagaaa
    48661 gctattttaa attatttact taaactttca aaagcacctt tagatgatga ttcaagagaa
    48721 gttgtggata ctttgtttaa tacagtcaat gatatagaaa gaattggtga ccacgctaaa
    48781 aatatagctg aattatcgca agttgcaata gattcaaata tttctttctc agaagaagga
    48841 caatctgaac ttgatgttat gtataataga gttgtttcag catatactta tgcattagaa
    48901 tcaatgagaa ctgataatgt tgatttagct tgtaaggtta taaaaattga agaacaagtt
    48961 gatataatgg agaaatcttg tagagctaat cacatgtata gattgaacaa taatctatgt
    49021 agtatagaaa atggagttat ttatttagac gtaataagta atttagagag aatatctgac
    49081 catgctgtca atatagcaca acaagttata gcaaagagat taggaaatga ttaaaaataa
    49141 ttaatttaaa ataatataaa atttaaaata gaggataaat aaaagagaaa ctttaaatga
    49201 tacgtttaga gtttcttttt ttgtaaattt atttgactaa aatttttaaa tttatacata
    49261 gttatagcat ttagaagaat cgatttcctg ataataaatg atgagatatg tatgattttg
    49321 tggcaaactt ttaaaaaaaa cacctatatt ttgtgttata atctctttaa aacgaatatg
    49381 ctgaaaagag ggatttaaat gggaaaaata aattttgatt atagtaaagc tacagagttt
    49441 ttttgtcaaa acgaaataga tgtcatgcaa ccatatgtag atgttgcgca cgacatgtta
    49501 cataataaaa ctggtttagg aaatactttt ttaggatgga tagaccttcc taaaaattat
    49561 gataaagaag agtttgacag aataaaaaaa tctgctgaga agataaaatc agactcagat
    49621 gttcttttgg taataggtat aggaggctct tatttaggtt cgagagctgc tatagatatg
    49681 gtaagtcatt cttttaggaa tggacttaaa aaagagcaaa gaaaggctcc agaagtatat
    49741 tttgtagggc ataatataag ctcaacatat attatggatt tattagatat aattgaagga
    49801 aaggatattt ctgtaaatgt aatatctaaa tcaggaacta ctacagaacc agctctggct
    49861 tttagaatat ttaaagatta cttagagaaa aagtatggta aggaagaagc aagaaagaga
    49921 atttatgcaa caacagatgc aagtaaaggt gcacttagac agttagctac agaagaagga
    49981 tatgagactt ttgtaatacc agatgatgta ggtggtcgtt tttctgtatt gacagcagtt
    50041 gggttacttc ctattgctgc tgctggactt gatattgatg ctatgatgaa aggcgcaaat
    50101 gatgctagag aagcatttca aaatcctgac ttaaaaagta atgatagtta tagatatgct
    50161 gttgctagaa caattttaca tagaaaagga aaagatgtag agttattagt gaactatgaa
    50221 cctcaactac attatgtaag tgagtggtgg aaacagcttt atggagaaag tgaaggaaaa
    50281 gaaaataaag gtttattccc tgcttctgta gatttctcaa cagatttaca ttctatgggt
    50341 caatacatac aagatggaaa aaggctttta tttgagacag tacttaatgt tgaaaactgt
    50401 aagagaaata taactataag ttcagaggaa gtagacttag atggtttaaa ttatttagca
    50461 ggtaagactg tagattttgt aaatcataaa gcttttgaag gaactctatt ggctcataca
    50521 gatggaaaag tacctaattt agtaataaat atacctcaac tagatgagta taattttgga
    50581 tatttagttt atttctttga gaaggcttgt ggaataagtg gatatttatt gggagttaat
    50641 ccatttgacc aaccaggagt agaagcttac aagaaaaata tgtttgcttt actaggcaaa
    50701 ccaggttatg aaaaagaaaa ggaagagcta gaaaaaagac ttaaataatt ttaaatagtt
    50761 aaggtttaat taagattaaa ataatataat gaaatcatca aaaaggagtt tactcaaaat
    50821 agatgatttc atttttttta tgaaaaattg ggaaaaatat attttgataa tttatattat
    50881 attaagatag ggagtggtat gagatgtcaa gaagaaagaa agggataagt ttagtaatat
    50941 tagttgctat aataagttca atacttagta gttttctaac aataatttta gttaaggaca
    51001 atttagtaag taaatctaca ggaagttcta cgccgatagt tgtaaatgat gatggtaaga
    51061 gtcaaaatat ttatcaagca gttgcagaaa aagctacgcc atctgtcgta ggtattacaa
    51121 caactagtgt tgatactagt aatatgtttg caataccaac tgaaacacaa ggtgtaggaa
    51181 ctggaatcat agttgattca aatggatata tacttactaa ttctcatgtt atatcagatg
    51241 gtcaagctac aagtgtaaat gtattattca atgatggttc aacaacttca ggtaaagtag
    51301 tatggtttga ccaacaactt gatttagcta tagttaaggt tgataaaact ggactaacac
    51361 cagcagaatt tgctgatagt gacaaagtaa aagttggaga tatatcaata gcaataggta
    51421 atcctcttgg attagatttt caaaaaacag ttactcaagg gataataagt ggattagata
    51481 gaactataca aacagaaaaa actaatatga ctggtttatt acaaactgat gcaagtataa
    51541 atgcaggaaa tagtggagga ccattgttaa atcaaaaagg tcaagtaatt ggtataaaca
    51601 cagcaaaagc ttctcaagca gaaggattag gatttgcaat accaatcaat acagctaaga
    51661 gtatagttga agaagtaatc aaaaatggaa aatatgaaaa agtaacttta ggcataaaag
    51721 gtacagatgt atcaaattat gaagcagcaa caggtacaaa attaagtact gataaaggtg
    51781 tttatgttgc agaagtaata tctggttcat cagcagaaaa agctggagta aaggtagggg
    51841 atattataac taaagtagga gatacagata taacaggtat gaatgattta aataaaaagt
    51901 tgtatacttt ctcaaaaggt gctagtacaa aaataacagt aaatagaggc ggaaaagcag
    51961 taacaataaa tgtgaatttc taaatatgac cagaaagata gagactacat ggtaaagtgt
    52021 agtctctatt tttatttaat ttttatagta tatacatcta aattaattaa aatgatgtat
    52081 tgtttatagt ttattgctat attttcttat aaaaataaat ttaataaata tttaaattta
    52141 atttacaaaa acaatataaa tatcataata aaataaaatt ttttaatatt actaaaaata
    52201 tatagacaaa ataataacaa atatagtatt atgtaattgt agaaatgctt ttcataggta
    52261 tattctatga agatgaattg cataaaaatg aaaaaatgga atggatatta tttaggggtg
    52321 aaaaatcatg aatccattag ttataaactt acaaaaatgt agcatacatg atggaccagg
    52381 gataagaagt acagtatttt ttaaagggtg cccacttgaa tgtgtttggt gtcataatcc
    52441 agagagtcaa acatatacaa aacaagtttt gtataatgaa gaaagatgtt caaaatgtga
    52501 agcatgtata aatatatgcc ctcataaggc aatttacaaa ggtgaaacaa aaatatgctt
    52561 agaccaagat aagtgtgagt tttgtgaaac ttgcttagac tattgtgtaa acaatgcaag
    52621 agagattgtt ggtcaagagt attctgtaag agatttggtt aaagagatag agaaggatag
    52681 aattttctat gaagaatcag gtggtggagt aactctatca ggtggagaag taatggctca
    52741 agatatggac ttcatttgtg gagtaataaa catgtgcaaa tcaaagggaa tccatgtagc
    52801 aattgataca tgtggctatg caaagtcaga aaattatgaa agagttgcta aatgtgcaga
    52861 tttgttttta tatgatatta agcttattga tgaagataag cacataaaat ttacaggaaa
    52921 atcaaatgac ttaatactga aaaatgttaa gattttaagt gaattagggg ctaacataaa
    52981 tataagaata ccattaattg taggagtaaa tgtagatgat gaaaatttag aggttaaaaa
    53041 aatgatagag tttttaaagc ctttgaatat acaagcagta agtcttcttc catatcataa
    53101 tattggtaaa cacaagtatg ataaaattta taaaaagtat gaaggagaag aactacaaag
    53161 accatcagaa gaaaaattag aggaaataaa aagactgttt gaagcaagta atttcaatac
    53221 taagattgga gggtaataaa atggcaagag gaacttttga gagaactaaa aaattaagag
    53281 aagaaagtat taatgcagaa ccacacatat ctattgaaag agcagtactt atgacagaag
    53341 cttataaaaa atatgaggga agtgtagaga ttcctgtact tcgtgcttta tcatttaagc
    53401 attatattga gaatagaact cttagcataa atgatggaga attaatagta ggtgaaaaag
    53461 gtgattctcc taatggagca ccaacatatc cagaaatttg ttgtcataca atggaagatt
    53521 tagaagtaat gcacaataga gatataataa attttagcgt atcagaagaa gctagaaaaa
    53581 tacataaaga agaaataatt cctttttgga aaaagagaca aacaagagat aagataataa
    53641 atgcaatgac acctgagtgg ttagcagcat atgaagcagg tatgtttaca gagtttatgg
    53701 aacaaagagc tccaggacat acagtttgtg gagatacaat atataaaaaa ggatttttag
    53761 atttaaagaa agacatagaa gcaagattaa aagagctaga ttttcttaat gatttagatg
    53821 catataataa aaaagcagat ttagaagcta tggcaatagc ttgtgatgca atggttatat
    53881 taggtaaaag atatgctgag aaagcaagac aaatggcaga agaagaaaca gatgaagcta
    53941 agaaaaaaga tttattacta attgctgaaa cttgtgatgt tgtaccagct cacaaaccag
    54001 aaacttatca tcaagctata caaatgtatt ggtttgttca tataggggtt actactgaac
    54061 ttaatatatg ggatgctttt actccaggaa gacttgacca acatctaaat cctttctatg
    54121 aaagagatgt agaaaatgga atactagata gagatagagc acaagaatta cttgaatgtt
    54181 tatgggttaa atttaacaat cagccagcac caccaaaagt tggtataaca ttaaaagaaa
    54241 gtagcacata tacagatttt gcaaatataa acactggtgg aataaatcca gatggtcaag
    54301 atggtgttaa tgaagttagt tatataatac ttgatgttat ggatgaaatg aaattgatac
    54361 aacctagttc aaatgttcaa ataagtaaaa agactcctca aaaattctta aagagagctt
    54421 gtgaaatatc aagaaaagga tggggtcaac cagcatttta taatactgaa gcaatagttc
    54481 aagagcttat ggaagctgga aaaactatag aagatgcaag acttggtgga accagtggtt
    54541 gtgttgaaac tggatgtttt ggtaaagaag catatgttct aactggatat atgaacattc
    54601 caaaaatact tgaattaaca ttaaataatg gatatgaccc aattagtaaa aaacaaatag
    54661 gtattgaaac tggagaccct agaaatttcc aatcttatga ggaactattt gaagcattta
    54721 aaaagcaatt acattatatg attgacataa aaatagaagg aaatgctgtg atagaaaata
    54781 tatgtgcaaa acacatgcct tgtccactta tgtctacaat agttgatgat tgtatagaaa
    54841 aaggcaagga ttaccaaaga ggtggagcaa gatacaacac tagatatata caaggtgttg
    54901 gtataggaac tataacagat agtttaacag caataaaata taatgtattt gataagaaaa
    54961 aattcgatat ggatacattg cttaaagcac ttgatgctaa ttttgaagga tatgaagcta
    55021 tacttaattt agtttcaaat aagactccta aatatggaaa tgatgatgat tatgctgatg
    55081 aaataatgca agagatattc aatgcatatt acaatgaggt aactggtaga ccaactgttt
    55141 gtggtggaga atacagagta gatatgttac caacaacttg tcatatatac tttggagaaa
    55201 ttatgggagc aagtccaaat ggtagacttt gtgcaaaacc agtttcagag ggaatttcac
    55261 ctgaaaaagg tggagatact aatggaccta cagcagttat aaaatcttgt gctaagatgg
    55321 accacataaa aactggtgga acattattaa atcaaagatt tgcaccatca gtagttcaag
    55381 gagaaaaagg gttagataat atggctaatc tagtaagagc atactttaat atggatggtc
    55441 atcatataca atttaatgta tttgataaaa atgtactgtt agaggctcaa aagaatcctc
    55501 aggattataa agacttaata gttagagttg caggatatag tgaccatttc aataatttaa
    55561 gtagaacact acaagatgag ataataggaa gaactgagca aacattctag gtttgcttgt
    55621 aaataaaact tcttactata taaaatcgta actgatataa taaagtataa atggtttgat
    55681 tccattttta aaattttaga ttaattatga gctaactttc taaataggaa gttagctcgt
    55741 taagtttaat gaaaaaatcg ttaaatttat atttagtaaa tcatacacat attagtaagt
    55801 aatacataca aaagaataat ttattatagt tataataagg ttatatttta taaaaataag
    55861 ataattttaa gcagttaaaa taaagtcaat ttagctactt taaaataaaa ctaatagaaa
    55921 aaaggagatt tgatatgaaa actttaggat ttattggttc agggaatatg ggaagtgcaa
    55981 tgataggtgg catagtacag tcaggattag taaaggcaga agatataact gtatcagatt
    56041 taagccaagt tgccttagat aaagtaaaag aaaaatttgg agtaaatact acaactgatt
    56101 caaaggtagc tgttacaaat tctgatgtgg taatagtagc acttaaacct aatatatgcg
    56161 aaaaggcttt aactccactt aaagaattaa tagatgcaaa taaaataata gtatctatag
    56221 cagcaggtaa aactatagaa agtctggaaa gttttatagg ttcagataaa aaaatagtaa
    56281 gagtaatgcc taatactcca gcccttgttg gcgaaggaat gtcagctctt tgtaaaaata
    56341 gtaatgtaac agatgaagag ttaaatatga ttaaggctct gtttgaatct tttggtgagg
    56401 cagaaatagt atcagaatat ttaatggata cagttacagg tattagtggt tcttctcctg
    56461 catatgtatt tatgtttata gaagctatgg cagatgcagc agtattagct ggaatgccaa
    56521 gacaacaagc ttataaattt gcatctcaag cagtaatggg gtcagctaag atggttcttg
    56581 aaactggaaa acatccaggt gaattaaaag atatggtttg ttctccagca ggaactacta
    56641 tagaagctgt taaggtatta gaagaagaag gctttagagc agcagttata aaatctattg
    56701 ttgcttgtat agataagtct aaagatatga gcaaataaca gatgtaatta agtttactat
    56761 atggtttctt gaattgtttt tttagtgctt atttatatct atacataaat gtatttggta
    56821 taagtaaaat aagagtaatt tactataaaa aggaatttaa tgattgagtg ataatttatt
    56881 acttaaatat taata
//
LOCUS       DAMJYC010000015        56797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJYC010000015 DAMJYC010000000
VERSION     DAMJYC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.016"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(160..1761)
                     /locus_tag="PC472_000989"
     CDS             complement(160..1761)
                     /locus_tag="PC472_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2726335.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1872..2549)
                     /locus_tag="PC472_000990"
     CDS             complement(1872..2549)
                     /locus_tag="PC472_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726336.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2562..3257)
                     /locus_tag="PC472_000991"
     CDS             complement(2562..3257)
                     /locus_tag="PC472_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2726337.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3309..4508)
                     /locus_tag="PC472_000992"
     CDS             complement(3309..4508)
                     /locus_tag="PC472_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2726338.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4769..6814)
                     /locus_tag="PC472_000993"
     CDS             complement(4769..6814)
                     /locus_tag="PC472_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726339.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7225..8034)
                     /locus_tag="PC472_000994"
     CDS             complement(7225..8034)
                     /locus_tag="PC472_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2726340.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8118..8651)
                     /locus_tag="PC472_000995"
     CDS             complement(8118..8651)
                     /locus_tag="PC472_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2726341.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8801..10150)
                     /locus_tag="PC472_000996"
     CDS             complement(8801..10150)
                     /locus_tag="PC472_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2726342.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMFIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10162..11544)
                     /locus_tag="PC472_000997"
     CDS             complement(10162..11544)
                     /locus_tag="PC472_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2726343.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11788..13002
                     /locus_tag="PC472_000998"
     CDS             11788..13002
                     /locus_tag="PC472_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2726344.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13179..15092
                     /locus_tag="PC472_000999"
     CDS             13179..15092
                     /locus_tag="PC472_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726345.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFNDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15363..16337
                     /locus_tag="PC472_001000"
     CDS             15363..16337
                     /locus_tag="PC472_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2726346.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16358..16633
                     /locus_tag="PC472_001001"
     CDS             16358..16633
                     /locus_tag="PC472_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2726347.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16646..17809
                     /locus_tag="PC472_001002"
     CDS             16646..17809
                     /locus_tag="PC472_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2726348.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYIVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18146..20425
                     /locus_tag="PC472_001003"
     CDS             18146..20425
                     /locus_tag="PC472_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2726349.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20520..21860)
                     /locus_tag="PC472_001004"
     CDS             complement(20520..21860)
                     /locus_tag="PC472_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2726350.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21872..22654)
                     /locus_tag="PC472_001005"
     CDS             complement(21872..22654)
                     /locus_tag="PC472_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726351.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22669..23691)
                     /locus_tag="PC472_001006"
     CDS             complement(22669..23691)
                     /locus_tag="PC472_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2726352.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGERNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23697..24710)
                     /locus_tag="PC472_001007"
     CDS             complement(23697..24710)
                     /locus_tag="PC472_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2726353.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24838..25776)
                     /locus_tag="PC472_001008"
     CDS             complement(24838..25776)
                     /locus_tag="PC472_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2726354.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26046..26510
                     /locus_tag="PC472_001009"
     CDS             26046..26510
                     /locus_tag="PC472_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726355.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26622..26939)
                     /locus_tag="PC472_001010"
     CDS             complement(26622..26939)
                     /locus_tag="PC472_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2726356.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26952..27683)
                     /gene="chbG"
                     /locus_tag="PC472_001011"
     CDS             complement(26952..27683)
                     /gene="chbG"
                     /locus_tag="PC472_001011"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2726357.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKSVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27709..29019)
                     /locus_tag="PC472_001012"
     CDS             complement(27709..29019)
                     /locus_tag="PC472_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2726358.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29096..30409)
                     /locus_tag="PC472_001013"
     CDS             complement(29096..30409)
                     /locus_tag="PC472_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2726359.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30431..30742)
                     /locus_tag="PC472_001014"
     CDS             complement(30431..30742)
                     /locus_tag="PC472_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726360.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30942..31646)
                     /locus_tag="PC472_001015"
     CDS             complement(30942..31646)
                     /locus_tag="PC472_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2726361.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31737..33098)
                     /locus_tag="PC472_001016"
     CDS             complement(31737..33098)
                     /locus_tag="PC472_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2726362.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33597..34751
                     /locus_tag="PC472_001017"
     CDS             33597..34751
                     /locus_tag="PC472_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2726363.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRSAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34833..36551)
                     /locus_tag="PC472_001018"
     CDS             complement(34833..36551)
                     /locus_tag="PC472_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2726364.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36556..37545)
                     /locus_tag="PC472_001019"
     CDS             complement(36556..37545)
                     /locus_tag="PC472_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2726365.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37641..38690)
                     /locus_tag="PC472_001020"
     CDS             complement(37641..38690)
                     /locus_tag="PC472_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2726366.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(38968..39636)
                     /locus_tag="PC472_001021"
     CDS             complement(38968..39636)
                     /locus_tag="PC472_001021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2726367.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39642..41015)
                     /locus_tag="PC472_001022"
     CDS             complement(39642..41015)
                     /locus_tag="PC472_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2726368.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(41012..42790)
                     /locus_tag="PC472_001023"
     CDS             complement(41012..42790)
                     /locus_tag="PC472_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2726369.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEIHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42907..43230)
                     /locus_tag="PC472_001024"
     CDS             complement(42907..43230)
                     /locus_tag="PC472_001024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2726370.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43217..44194)
                     /locus_tag="PC472_001025"
     CDS             complement(43217..44194)
                     /locus_tag="PC472_001025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2726371.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44209..44772)
                     /locus_tag="PC472_001026"
     CDS             complement(44209..44772)
                     /locus_tag="PC472_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2726372.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44824..45315)
                     /locus_tag="PC472_001027"
     CDS             complement(44824..45315)
                     /locus_tag="PC472_001027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2726373.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45318..47243)
                     /locus_tag="PC472_001028"
     CDS             complement(45318..47243)
                     /locus_tag="PC472_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2726374.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47245..47556)
                     /locus_tag="PC472_001029"
     CDS             complement(47245..47556)
                     /locus_tag="PC472_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726375.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48186..48461)
                     /locus_tag="PC472_001030"
     CDS             complement(48186..48461)
                     /locus_tag="PC472_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2726376.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48576..49985)
                     /locus_tag="PC472_001031"
     CDS             complement(48576..49985)
                     /locus_tag="PC472_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2726377.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50356..51018)
                     /locus_tag="PC472_001032"
     CDS             complement(50356..51018)
                     /locus_tag="PC472_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726378.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51272..53491)
                     /locus_tag="PC472_001033"
     CDS             complement(51272..53491)
                     /locus_tag="PC472_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2726379.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLNEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53767..56421)
                     /gene="adhE"
                     /locus_tag="PC472_001034"
                     /gene_synonym="adhC"
     CDS             complement(53767..56421)
                     /gene="adhE"
                     /locus_tag="PC472_001034"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2726380.1"
                     /translation="MAHKEQVIIENKKQNEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        18871 a         9923 c         6825 g        21178 t
ORIGIN      
        1 attttttcac tataaagtat aaatattatc tgattattaa aataaatatt aatactataa
       61 ataaaaacga agcatgattt ttatttaaat aaatctcaaa cttcgtcttt acttacttag
      121 atatttattt actttctaaa tcctattatt tttaaaacct tatcttttta atacttcacc
      181 acatttattc tcagtaatct ctccattttc caacgcaatt tttccattta caattacata
      241 ttcaacacca ttggctagta attgtggctt tgaaaaatca atatcttctg acctagaata
      301 actatagtta tccatgtcaa taactgctat atcagctatc aacccttctt taagaatacc
      361 tctatttttt attttaaatt gcctagctgg taatgaagta actcttctta caatctcttc
      421 caatctaaca ccagactttc tgtacatatt gaagaaaatt ggaaaagctc ctcttctttg
      481 tagttcaaac catgacatat ccccacccac actaaacaaa caatctgaac caaccataac
      541 aaatggatta tcaattattt tatcatcatg tagtttatct gggaaatcag ctctatacat
      601 tcctcctaaa caaaagataa gttcttcgtc tccatctaaa agtaaatcga gtaataactt
      661 gtcttcatct attccttttt gtgtagctat ttcacgtatt gatttctttt ccatattaat
      721 atctttagtg tttagaagta ttatactccc cctatctcca agtataaatg cttcttttaa
      781 tataagtgtt cttccctctt catttctaag agcatccatt accccattta tacctaattc
      841 aaatagacta gatgatattg ccataataaa ttgaattact ctttttttct tcatacaatg
      901 cccagaactt cttggtactg tgtcaaccat tatttcaaca ccattatacc tagcctttaa
      961 tatctcatca aatgcttcta tttcaactgg acttaagtga gatacttgaa ccctagcacc
     1021 acttttttgc ccaacctcta tggcttcttt tactgcatta tatcttccta tgtaatctct
     1081 aatatgagat gtatatatac catcatgctc tttaatctgc cttgcaatat caacaagttc
     1141 atcaatatca gcatacttac ttggtatata tgccaatcca gtagatattc caaaagcacc
     1201 acttttcatt ccatcattta ttatatctgt aatttcattt ttttcctttt ctgtaggagg
     1261 tctatcctta gaacctccca taacactcca tcttatagtc ccatgcccca acaagaatcc
     1321 catattgaca gaaataccac ttttagatat aaggtcacaa tattctgtaa aattattcca
     1381 acatttattt ttatctataa gatacttttt ttcttcttca agtaatagac catttttata
     1441 catatattca tatacatttt cagaagaata tggtgttata gaatgtccac aattaccatt
     1501 tatagttgtt gtaactcctt gttttagaaa cttttcaaat ttaccatcta gtattgctac
     1561 tatttcttca tgaacatgag ggtcaataaa tccaggtgtt ataacttttc cacaacaatc
     1621 aatctcttta tttgtattgc tatttatctt agaatctatt tttgctatta agttgtcttt
     1681 aattgctata tctccataaa aaccaggatt acctgtacca cctattataa atccattttt
     1741 taagattaaa tcatatatca aaatattccc tccttaaaaa ccaattttta aataatctta
     1801 atgtaatttt tatatttgaa aatcaaatta ttatatttca tatttgaaaa ttcactatta
     1861 tttcattgta attagtcact aagcttatct ttatacatag ctcttgcaat taatatagta
     1921 ccaaatacac atataagaac tactagagac tgtggtatta taccttgctt tgataatata
     1981 atagtcaata cagatattac aattgctact aggcctaatt ttttatcttg taaaaatact
     2041 tgtccaaaaa ctgacccaaa tagagctggt aaaattaagt ttaatttttc tactacttca
     2101 gctggaattt tagatagtac gcttcctcct aaaattaccc ctatagttag tatacttata
     2161 ttcactaaaa tagatacagc tattcctatt gtagatatta tcgagccttc ttctgtacct
     2221 gattcaactt ctgctgcctt ttgagctgct attgaacatg gtagcctcat attagatata
     2281 ttaccagaaa gaaaactcat ataagttcct gggataccta agattggtga ataagaaact
     2341 ggttctatta tgtaatttgg agcactcata ctgacaattg ccattgtact tgctaataat
     2401 gctgatattg gaggcataat accaaatcca aagctcaaaa ttattccagg aactaatgct
     2461 gctataatcc ccaaagatag tgttagtctt ccatacctta tcataaaagg taaatattct
     2521 tcattgtata tagattttac tttactcata aaactccccc cttatattac tgaagctatt
     2581 atcattccgc taaacattga tattgtaagc ccccactctc ttaaccattt tatatttttc
     2641 ttcttttcaa gcatacacaa tattaccatt atagccattc cacttataca tgcaattgtt
     2701 ccattatcaa aacgcaacac cctatcagca tttagatatg caaatgaacc caacattgct
     2761 cctagagata ttattggtat aaatgatttc ttgccactag atataacatt ggttatctta
     2821 tctagtttat gtccaaatag taatgtgaat attatccatc ctaaagaacc taatatcata
     2881 gtccaaactg catttgtaaa tgcaagtttt gtcatttcag gagaacctaa agttatacct
     2941 aatgcatctg ttccaaatcc ggctgacatt gattcaaaaa ttacagaacc tataaatgat
     3001 aatctcatcc atgctatagg cccacccata gttactataa gcgataccat tccagcaaat
     3061 atagtcattg aaggcccaat agctgaaata gcactactct taacagctga ctttatttgt
     3121 ttatctgtta ttcccattgt tttaccaact ttgcaagatt ttatagcaaa tataagggat
     3181 tgtaccacta ctattgatac tgctactgta ctcgctatcc acaaaagtgg atgattagct
     3241 aattttaaat aatccattta taaaacctcc ctataaatcc tcaaatttta ttaatatttc
     3301 taatatatct atttccaaac ctgttttagt actctaagta tatttccacc tagtaccttc
     3361 tctatttcct catcactata tccatgcttt actaaatatc taacaatgtt ttttgaagtt
     3421 tctgttgggt tctcaagacc ttttacatat tctactttgg gatgttcttt tgttcctgtt
     3481 atttttttta tatcaaattt agcagctagt actgagtgta attttacatg gtcgccatat
     3541 actgtatctg gtccaaaagc tacatggtct attcctacta aatttttaat atactcaaaa
     3601 tgttccataa atgactctat tgaatgttct ttattcttct ctgttattgt agtatgaggt
     3661 gctgcttcta cacctataac ccctcctttt ttagcacatg ctattaatac ttcatctgga
     3721 gttagtctct ttgtgttcca taaacttctt gccccaacat ggcttaatat tattgggtcc
     3781 ttgctatatt ctattacatc aagtgtagtt tgaactccaa cgtgagagca atctattgcc
     3841 attccaattt tattcattct ttcaacagct tttttcccaa aatttgtaag acctccatct
     3901 ttctcttctt ttagtcctga acctaatgca ttggattcgc tgtatgttat acccattaac
     3961 cttataccaa atccatacag tatatctatt ctatcaagtt cattttctat tggtgctgca
     4021 ccttctaatg ttggtatcaa tgctacttta ccttcacttt tagctctgta tatatcttct
     4081 actttttcac actttatgac aaaatcttga tgagctaaat cacataatct catgcctaaa
     4141 tctattaata tatcatccca tttccaccct gaatttgatg ttatcgtaca tacaccatcc
     4201 attaaattat caaatatagc atcataatat cctcttgata atgcttcata agcacatctt
     4261 tgtttacctt ccctattata ttcaaatata tctctttcta ggtgttctgg aaataatact
     4321 ggatgttcat gtaatgatat aaatattttt tcatccacta tacttttaac cttagcttct
     4381 tgaatatcat ctaattcaat aaggtattct ttaactcttt tatctccttt acacatctca
     4441 atagatgaaa tatcttttcc tttttctaaa taactatagg cttcatatcc tttgtaagct
     4501 gacttcataa caacatcctc caagttctac aattttttat atttttccta tgtagcaatt
     4561 gatgtgccaa aatataaatt ttaattgtat aataaatctt gcaaaaaata aaaacagtag
     4621 taaggttgat atttactact gttttattaa aaataatctt cttagattat ttattcaata
     4681 tttttgaatt atcttaatct taaagataat tttatttatt tctatccatt atagggattt
     4741 ttttagggtg ttttgccttt ttagtcccct atatcactcc cttaattttt acatatgata
     4801 taaagttctt tccttcatca gttataaaac taccttgttt ttttactcca gataaaagta
     4861 aaccatggct ctttaactta tctattcttt ttctaatttg gtctacactt aaaactatat
     4921 ctttttcttt taaaatctct tgcagtttat ttcttcctaa taatatattt ttattattcc
     4981 aagtttctat actggtcaat atacatatac tttcctcaaa gaaattagat tgtttgaaat
     5041 ctaagataat tgaatcgaaa ttttcattaa ctaaagtatt gtttttttca aatctaaatt
     5101 gttctggcaa atgctcgtaa tctactctat cttcctcaac tatagtatca atataataaa
     5161 ttaaattttt aagctctctt acatttcctg gccattcata caatgttaaa gcttgcattg
     5221 attcctctgt aaaacatttg ttgctattta tttcatctaa aaaatactta cttataagag
     5281 gtatatcttc cttacgttct ctaagtctag gtatctctat atgtaataca ttaatcctat
     5341 aatacaaatc ttctctaaaa gaaccttctt taattttttt ctttaaatct ttatttgttg
     5401 cagctataat tctaacatct attggtataa ttttacttcc gcccattctc ataacttctt
     5461 tttcctgtaa tactcttagc aatctctgct gtacatctaa agatatatca cctatctcat
     5521 ctaaaaaaat tgttcctgta tgtgctcttt caaatatacc catttttcca ccttttattg
     5581 ctcctgtaaa acttccttca tcatagccaa aaagttcact ttcaataagt gtatctgaca
     5641 aagaagatag attaactgca acaaatggct tgttttttct tagagattca ttatgtattg
     5701 cctgggcaaa tatttctttt cctgttccat tttctccaag tatcaacaca gaaaagtctg
     5761 tagttgctat ttttcttgca atattaattt tttcttttat tattttactc tcacctacaa
     5821 cactctcaaa tgtatacttt gatacaaaac cttttgactg aagtttattt tgaatcttgt
     5881 cctcaagcac ttgaattgca cttatatttt taatgcttat aatactcttt attcgttcat
     5941 tattttcata aacatttact ttattaatta tcagtttttt gttatttaaa tttactactt
     6001 catcattaac ttcatcttct tgaaaaaata tttttttgac aaccttatca ctaaataagt
     6061 ccataaagtt attagatata atttcatttt ggtctattcc aactagatta gaaaatactt
     6121 tgttgcaata tataaatttt cctaatttat caacagaagc aataccttca tctataatct
     6181 ccaaaaaact cttcatggtt ttattcatag ttgtataata cctatatcca cttactgtat
     6241 cttcattgta tttttcctta ataatatgta gtttatctat agatatattt agttttgtaa
     6301 agatttctat aactgtattt atatcaatta ccttatcacc tatgtcaatt atttgcttta
     6361 tgttttgtgg tatgctgttt ctgcttcccg taataattcc aatctcacat ttagacttat
     6421 cacaacctgg ataatatggt attagattaa tatgaattaa tcctagtttt ctaattatct
     6481 tggcagtttc atctgctgac tctttataag catctataac cataacatca gaatcatttt
     6541 ctatacttat tatctgattt atattttcaa tatttatagt acgatgtact attacaatag
     6601 gtatattttt atcaatatta ttatgtatat attcctttat atcattgtct gaacatacta
     6661 caagctcata gcttgacagt tcaatattta tcctatttac atataccaag ttatctatat
     6721 tacaaaattc tccaaatata ttttccaatt gttttgatat atctatattt attttgtcat
     6781 cagttgatac aaataaaata ttatgttttt tcatagtccc acctcctgtt taacgagtat
     6841 agaaagtaat aatttggtct attgtagcac tttttaaaaa agttttaaat cttaaaaatc
     6901 aaattttgta ccatttacta ttattttcca taataaaata atgtaagcta tcttttttca
     6961 catataattt aatttctaaa acggttagtt tgacattatt ggaatataaa caaaaattta
     7021 atataaattc gttaatatat taatacattt ttaagaatga caaggaataa attataacaa
     7081 attttaggaa tatataataa tagggtctca gaacttagtt acaagttcta aaaccctatt
     7141 tgtttagtgt aaaaaacatc ataaaattta gcttaaatct tttcacataa tattactctt
     7201 tttctaagtg ataataaaat atatctattc ctctaaaaac tgtaaaaatt cctgattaaa
     7261 tagtttaagc tcatcataga atatagcatg accactatac tcaaaagtat ataaaattga
     7321 atcttctatt ttttcattca taaaaactgc aaattcatac ggacatattt catctaattt
     7381 cccatgaaat ataccagtag gcactcttac acattttaaa tcattaaata attcttcatc
     7441 tcttaaagat acagcagttc caatagtgcc aatacctgat gcactaaaac caatatcatt
     7501 aaaccatctt ctaaagcttt ctggtggatt tgatgcaaat actttttcgc caaaatcggt
     7561 taccatttct ggtctatcag tacatgcttg tgctatcaat ttatttacat cttcacgtgt
     7621 cataccatat ggaaattctg gaggtctttg tacaaatgat ggtgcagcag ctgcagctaa
     7681 taccaatttt gacactccat atccattaaa taaactcata tacctaagta cgattgctcc
     7741 tcccattgaa aaaccaacaa gggtaaagtc ttttaatcca atagcatgta ccaccttata
     7801 aatatcgtct gctaattggc tataagtata tcctcctgat gttgcatcag atttaccaaa
     7861 acctcttaaa tcaattgaaa ctgttctata tccgagtttt ggcaatatat ttgtttgata
     7921 ttcaaatatc ttatgtccta agggccaacc atgtataaaa agtacagttt ttttagcact
     7981 cggattcaaa tcatatactg ctatgtttat atcattcaca tatacataat acatatcaac
     8041 agcaactccc ttcaagaaaa tgtatgatgg ttattttata tatatgaata aaatgatgaa
     8101 taactaaatt ttcaaattta tttacaatac ttctctattg ccttttcaaa taaacttaca
     8161 tgaagttttt catctaggat tattctattt aatatagctt ttatataagg gtcttcaata
     8221 agacttatat ctttataata attattaata gccacatgct ccaaatgcaa atctagtttt
     8281 aatctgttac aaatagaatt tccataacat acaaaaccac cattccaata tgcaccacaa
     8341 gaacttgtag aacctctata tacaggattt ccacccaata atcttatagt ctttgctaat
     8401 atctccatat gtaacatttc tgttatagat atatttatcc acatttcact taactctcta
     8461 tgcgtttcat ctaaatcaaa attatggtat agatattgat ttacactcgt aaattcactt
     8521 gatactccgg catagtcatc catcaataat tctgcatagt acttatttgg acctaaaact
     8581 tttatctctg gatatggctc atcacttgaa tatccatttc gcttgtgcat gtcataatgt
     8641 gcatcactca taatatctcc tcctttaaat ttaatgtatg atattaattg tatgcatcac
     8701 aactttttca tataacttag ataataaaat ctacttaaat aataaaaagc catattaatt
     8761 aaaattaaca tgacctttta tccacaaaat tactttttaa ttattcatct tttccagcta
     8821 ctgatagctc tttaataaca actgaaggtg aacctatact actcattgga aattctaaat
     8881 cttcccctat agcttcaata tctttcaaca ggtcaaaata gtttcctgct actgtaattt
     8941 gctctactgg aaatactttt tttccttctt tgatataaaa gcctttggca gctaaagaaa
     9001 agtctcctgt tactgaattt gcacctgaat gaagtcctgc aaagctagta accatcaatc
     9061 cttcatctat atcctttata atttcatcta aagatttatc tcctttctct atataaaaat
     9121 tagttggata tatgcttata ggtgatgaat atgaagattt tattccattt cctgtagtct
     9181 ttacacctgc cttattagct gtttttaagt tatgaagtaa agttataagc tttccattta
     9241 aaacaacttc ttttttaaat gtagctactc cttcatcatc aaaaggagta gatgccatac
     9301 cattttctaa aagtgggtca tcaactattg taactatagg agatgctatc atgtctcctt
     9361 ctctattttt caataaagat aatccttttt gagctgaatc tgcattaaat atcccagaaa
     9421 atgtgctaag caaagatacc atagcttcat taagcaatat agttttatat ttacctgatg
     9481 gaatactctt accacctact ttagataaag cattatcaac accaagtttt gctatctcac
     9541 aaggttttat ttcttcaata gaatttgcta tattatatcc tattccatca tatttttgtc
     9601 cattgtcttc tatgatagga actacaaacc ctataaacat attagtttta ttgcttaaat
     9661 taagaccttt agtattagat ataccattac ttgatacaga ataagatata gtacatttag
     9721 ataaatttac caccttatct gaataagctt tagtctctct ttccatttct aaagctaaat
     9781 caatcaactt atctgcctct aaattttcta gctctttaga ataagtcttt acatcattat
     9841 agtgcttatc tccttcatat ataaattgta catcttcatt ttcaatagtg tttactccat
     9901 cttttacatt ttttatcaac atatcaacag ctttatcatc taaaatttct gtataagaat
     9961 aacctatctt tccatttacc ttacctctaa aagataaacc aaaagattta tcaagattgt
    10021 atttctcaac ttctccttca tatatattta tacttaaatt ttctcctgta taataatata
    10081 tttcacactc ttcaaaacct tcacttagag cctttttaaa taatatatct ttaaaatttt
    10141 taagttccac aattatccct cctatcttcc acctacagta atttctttta cccttatcat
    10201 aggttggcct acattagttg gtatacttcc tgatgatgag ccacacatcc cctgagcttg
    10261 ttttacgtta ttcccaacca tatctatatt cataagtata tcgctacctt ttccaattaa
    10321 actagcacct ctaactggct cttgtatttc tccatttttt attaagtaac cttctgaaac
    10381 tgcaaaatta aactctcctg ttacaggatt tacagaacca ccacccattt ttttagcata
    10441 aagtccatct ggtattgact ttataatatc ttctggttta tcctctccag cagcaatata
    10501 agtgttagtc atccttgaag ttggtgcaaa tttgtagttc tgtctacgag aacttcctgt
    10561 aggattcatt cccattcgtc taccattaag tttatctatc atataagact tcaaaattcc
    10621 attttcaatc agtatattct tttgagttgg atttccttca tcatctatat tcatagaacc
    10681 ccagtagttt ggtattgtac catcatctat agctgtaatc tttgtggaag caatctgttg
    10741 acctaattta tctgcaaata cagagttccc ctttgcaact gatgtagctt ccaaagaatg
    10801 tccacaagct tcatgaaata ttactccacc aaatccatta tctattgcta ccatcatatt
    10861 acctgcagga caatttttag catgcaacat agtatgcgct actctagctg attcttttgc
    10921 ataatattct gggtctattt cttcaaatat ttcaaaccct ttacaacctc caggaccttc
    10981 aaatccagtt tgattttcat tgcccttaga tgcaattgaa cttatcccca gtctggttct
    11041 tattcttcta tcctcaacat atataccttc agtgtttgca attaatattt tttggtcttt
    11101 atccaaatag ctaacactta cttgagatat atcattacta aattcttttg cactcttata
    11161 tgcaaccttc ataacactta ctttatctct atttcctatt gaatttggat aaatttttat
    11221 attatgtatg ttatttattt taatactgtc atttaatatt acactttttc catcttccaa
    11281 ttttcctaaa gcaacagcag ctttgtatgc tacatccaac agactattta atgaattgtc
    11341 acttgtataa gcatatatac ttttcaatcc tttaaatatt cttattccta tgccataatt
    11401 tcttccacct attgcatttt ctactttatt atctattaaa ctaatagaat tatttatagt
    11461 gtcttcttca aaaatctcag caaaatctcc tccagtaatt agacactttt ctaatacctt
    11521 agaagctata ttttttgaaa gcataaattt acctcctact ttaaattttt aattaacttt
    11581 tattcaatct ttttacttgt taacaaacat tatatactat attacttaaa ataaggtaat
    11641 tatgaatata ttatacatat gttttatact aaaatgttat cacattttat ctatacatac
    11701 ccatataact agataattaa aatgtataat attatttgct atagaaaata atacactcat
    11761 acataataaa ttttaggagt gaagtatatg aatcaagaat atgtaaaagg aaatcctatt
    11821 tttactaata ttaataaagt tccaagacaa tttccatacc ttactgatga tattgatact
    11881 gatgttataa ttgttggtgg tggcgtaact ggatgtatct gtgcttacta tctagctaaa
    11941 aacaatataa agtctgtcat tcttgaaaaa ggaaggattg cacatggaag cactagcgtg
    12001 acaacttctc tattacagta tgaattagat gacaatttga tagatttaac agaggttatg
    12061 actctaaaag atgctctaaa agcatataac ctttgtactt cagcacttga agaattagat
    12121 acatttatag agctatatgg aaataagtgt gattatgcca aaagagatac tctactttat
    12181 actgccaaca aacttgaagt taaggctatc aaagaagaat ataacttaag aaaagaaaat
    12241 ggatttgatg ttgaatatat tgatgaatcc acaaaccctt ttagctttga tttaaaatca
    12301 ggattgatag caaagaatgg aggacgagaa ttagacccat ataaatatag tcaccatttg
    12361 attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt aaaaaaagta
    12421 gatttatcaa atgataaggt tactgctgaa gtatcgtatg gatacaaagt tcatggtaaa
    12481 aaattaattg tagctacagg gtacaataca agtttattta caaaaagaaa ttttgcaact
    12541 aagtcaaata cttttaatat agcaacgaaa ccacttaaaa acatagcttc ttggaaaaac
    12601 aacattttaa ttagagataa ctgtgaccca tataattatc taagaactac taaagacaat
    12661 cgattaatta taggtggtga agatgtaagt tttgatgata tagaaaatga aactttagcc
    12721 aatgaaaagt acgatatttt agaacaaaga ttgaaaagta tgtttaagga tataaaagac
    12781 attgaagttg aatataaata ttgtggttgt tttgcttcaa ctttagataa tttaggtttt
    12841 attggtccag ataataaaaa taacaatcta tggtattgct taggttatgg agcaaatggt
    12901 atactctttg caatattagg aggtataatg ttaagtgaat tatatcttgg caaacaaaat
    12961 aaaaatatga aattatttaa agtagataga tttgataaat agatattata tattaaaaga
    13021 ctgtaaaaag atgaaacttt ataattctaa aagttaactt tgatacagtc ttttataatg
    13081 ttccttttaa aaatacttta tttataatta aatataaagt aaaatgttaa aatataactg
    13141 ataaaattta gtcataagat aatatttaag tttatttgat gaaatatgga ggaattatta
    13201 tgaaggaaat actcgttatt gctcccacta aaggaaccta tgaaaaatca attcacatag
    13261 ttaaaaaaaa caaatataca aatattgatg ttgtatttgg caatctaaaa gaaggtatac
    13321 ctcttgctga aaaaagtatt aatcatggta ctagaattat cataagccga ggtggaactt
    13381 acaatatgtt aaaagctact tacaatatac ctatagttga aattaaagta gatgcttatg
    13441 atattataaa aagttacaaa gaggtaaaaa attccaacga accatttgga ataattggat
    13501 ttaataatgt catctacggt ttcgatatta tagaagaaat attgaataag aaaataacta
    13561 tgattgaaat tgaaaaagaa gaagaaattt atgatgctat agaaaagtac agaaagaaag
    13621 gtataaacac atatatagga gataccactg ttgcacatat tgttaagaga cttaattgta
    13681 agggaatatt aatcgaatct agagaagaaa acatacttag agccattcaa caagcagaac
    13741 aaatactcga agcaaccaag gatgaacaaa aaagaagatt acagatagaa gctatgacag
    13801 attttgtaca tgatggaata ataacagttg ataaaaattt taaaataaca ttatttaata
    13861 agagtgctga aaaaatattt ggtataaaaa aagaaaatgc actttatcat aatgttgtag
    13921 atgttatccc aaacacagtt cttccacaag tagttgagac tatggaacct caaattggtg
    13981 aaatacaaaa tatagggagt acaaaaatta ttacaaatag agttccaatc attgttgatg
    14041 gtcagattca aggagctgtt tcaacatttc aagatagcaa ggagataagt tcttttgaac
    14101 atacaataag aaggagctta ttaagcaaag gtctatctgc taaatacact tttaatgata
    14161 taattcatgc tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt
    14221 atgatactcc tatgattatt actggagaat ctggagttgg taaagaacta ttttgtcaaa
    14281 gtatacataa ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca
    14341 tacctccatc tctaatagag agtgagtttt ttggttatga ggaaggttca tttacaggtg
    14401 ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca tgaaggcaca atcttcctag
    14461 atgaaataag tgaaatccct cttgaacttc aaggtcgatt attgagggta ttgcaggaaa
    14521 aacaagttat gagaattggt ggtgataaag ttattcctat agatgtaaaa attatctgtg
    14581 cttcaaataa agacttaaaa aatatgatgc gcaatggttt atttagacaa gacctttatt
    14641 ttagaataaa tatactaaca ttatatcttc cttcattaaa agaaagaaaa gaagatatta
    14701 taaaattatc agaatttttt atacataaat attcaagcaa atacaataaa acgcctttaa
    14761 ttatcactcc aaatattaaa aaggcacttc ttgaacgaga atatgaaggt aatataaggg
    14821 aattggaagg tatgatggaa agggctgtta ttgtggaatc atttgactcg attttattag
    14881 aaaagcttag caatgaatat acaaataata tagttgataa agataatagt gattttgaat
    14941 gcagtaattt agatttaaaa actttagaaa aaaaatatat ctataaaatt tataacgaaa
    15001 ataatagaaa tactacaaaa acctgtgaaa ttctaaagat aactcgctcc actctatggc
    15061 gtaagttaaa agaaatagaa gatgatgttt aaaaataata catatctata tatatcaaaa
    15121 cgttttatgt ttaattttaa tacataagac gtttatttgt tttcaataaa aaatacaatt
    15181 atatgataaa aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt aattataagt
    15241 aatctacagc atttcaatcg attattaaaa ataatcataa taactaaaga tacaaactct
    15301 atatattggc atagtaattg cttttataat acctatataa aaatgaaagg gagtgatact
    15361 agatgcacat cttaaaatca gtgaaaaaga taccaggagg acttatggtc tgtcccttgt
    15421 tatttggagc actatttaat acagtatgtc cacaagctct tgaaattgga ggattcacaa
    15481 catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta tgcaatggag
    15541 ctcaaatcaa tatcagacaa gctggtaaac cattgactaa aggtatagta cttacagcaa
    15601 caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt ggacctaaag
    15661 gtataattgg tattacacct cttgccatag ttgctaccat gactaattca aatggaggtc
    15721 tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca atatctatct
    15781 tatctctaaa tgatggtcct ttttttacaa tgcttgcttt tggagtaact ggacttgcaa
    15841 atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga tttatacttg
    15901 gtaaccttga tgaagatata cgagaatttc ttgcacctgg tacaacatta ttaattcctt
    15961 tcttcgcttt tcctttagga gcagcattaa actttaatca aattataact gctggactac
    16021 ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg tactttgtaa
    16081 tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca acagccggaa
    16141 atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct acaatagcag
    16201 ctgtttcaac agtacaggtt gctgctgcta ttattgttac agcaatatgt tgtccaatgt
    16261 tagtttccta tctagataaa agagaaaaaa agaaaaaatc tatttttaaa acaaataaaa
    16321 atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa
    16381 tgaaaaagat aatctagtta catgtgtcag acctttagtt aaaggtgaaa aagtttcaat
    16441 tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga tggctacaga
    16501 agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa attataggaa ttgcaacagt
    16561 tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac gtggtcgtgg
    16621 agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag aaaatggtca
    16681 gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa gtgagacagc
    16741 tacaagaata gcatcacttg tgcctggagc aatagcaatc cctcatcaac atggatgttg
    16801 tcaaattggt tctgatatag agcttacagc aaagacatta ataggatttg gaaaaaatcc
    16861 taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag caaaagaatt
    16921 agcatctgaa atagctacaa caggaaaaaa ggtagattac attgttatac aagaatgtgg
    16981 cggaacatta aaaacagttt ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt
    17041 atcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac ttgaatgtgg
    17101 tggttctgac ccaacaagtg gaatagcttc aaacccttca ataggcgttg catcaaacct
    17161 tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa ttggtgcaga
    17221 acatttgttg gctacccgtt ttgaagatga agaaatgaaa gataagtttc ttaaatttgt
    17281 aagtgatgta gaaaaaagag caattgctat gggagaagat ttacgtagtg gtcaacctac
    17341 tccaggcaat aaaactggtg gactttcaac aattgaagaa aagtcacttg ggtgtatgta
    17401 taaggcaggc aataagccat tcaaaggtgc tcttgaatat gctgatattg tacctcctga
    17461 taaaaaagga ttatatttta tggatactcc aggtcaagat atagattcta tcactggtat
    17521 ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc caacaggaag
    17581 ccctatttca ccagttatta aaatcactgg gaatagtgat acatacaaca aaatgccaga
    17641 caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag cagatatagg
    17701 tcaagaaata tttaatgaaa taatagaagt ctgtaatggt aaacatacca aagctgaaag
    17761 tcttggtcat agagaatttg gcatttacag aatttcatct acattttaga ttgtaacaaa
    17821 atatctttta taaatataat acttatgtag atacataagc taaaagttct ttctaatact
    17881 ataaaaattt tctatactga aaaggaactg tatcaaaatt attttgaaaa cagttctttt
    17941 tttaacctct tttactcaaa atattataaa ataagattta aacaataaac gtatcttgat
    18001 taatctctaa gaagacataa ataaagctaa atgtaaagtg ggataaataa aatttaatat
    18061 ctttgtatga aaagatattt ggtataatat gtctgagaga attcttattt gtaaacaaat
    18121 aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata aaaaaataat
    18181 agctacttta ctattgacac ttcttttaat cgtgtcatta acatgtgtca cttttattat
    18241 gaatctaaat aaagtaacac ataatgaaac agaacgatat cttgtagaag tttcacaagg
    18301 tatttccact acaattaatg tgaaatgtaa aagtatattt aggacactat attctatggg
    18361 agatacttat tttgagatta atgatagtga aaaagaagtc aatagatact tatcaaaaaa
    18421 attaaaatta tttgaatttg attggttagg tattatagat tctaatggaa attttgtaag
    18481 tgcccttaaa gatacaggta atgttaaagg acaagacttt tatagaaaag cccttcaagg
    18541 caaagaaaca attactcctg ttaaatcaca atctggtaca tatggaattt tatattctgt
    18601 accactatac aatggcgata aaattgttgg agctgttgct gcttggaata ctattgatac
    18661 tatgagaaat ttattaagtg tagaaagctt tcatggtgaa ggattttccc aaattatcga
    18721 ccaagatgga aattttattg taagttcagc aaacaaaaat gctccaaaaa atgtggtaaa
    18781 tttttttgac atgattaaac accaaggaaa acaggttgat aaaaataaac ttgaagaaat
    18841 gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat tatacattaa atgatggtat
    18901 ttctaaggca atgcattatc ttccattaca agaagaaaac ttatacctcc tttcaattgt
    18961 tccaacaaaa acagcaagtg cacagtttga tttacttcta aaaaaagcaa tcttcatcaa
    19021 tattttaatt gttatactat ttttattgct tattatattg attatgttta taaatcaaaa
    19081 gaacagggca caacttataa aaatcgccta tgtagactct gtaactaaag gttacagtaa
    19141 agcacgtttt gatattgaag ctaaaaaaat tatacagtca tcttcatcag aaagctattc
    19201 attggtatct ataaatattc agaaattcaa acttattaat gattcttttg gtagtgaagc
    19261 aggagataaa acattaaagt acatacatga tgtcattagt aattatctag aaccaggaga
    19321 attgctttgt cgagtttcta atgaccagtt taatgtactt attaaaacta ttcctcaaaa
    19381 agaaattctt aagtacatag aaaatatcac caatgatatt aatagtttca ataaaaatga
    19441 agaatgtaaa tattttatag ctctatctat aggtgtatat aaaattgatg acccaaatct
    19501 acctttagtt tcaattcaag accgtgcaaa tgttgcacga aaaaataata aaggaaacat
    19561 tggaaatcat ccactttata catgtgtttt ttatagtgat ttagagcgtt taaaaatgat
    19621 taaagaaaaa gaaatggaaa atagaatgga ggaagcatta aataataacg aatttatagt
    19681 atatcttcag cctaaaataa gcctcaaaga taatactatt gtaggtgcag aagcattagt
    19741 aagatggcaa gacccaaata aaggtcttat accaccagac gagtttattc ctttctttga
    19801 aaaaaatggt tttattacaa aactcgacca ctatgttttt gaaaaagtat gcagtatgat
    19861 aagaaaatgg atagataatg gagaaactcc aatacctgtt tcagttaatt tttcacgtgc
    19921 taatttaaat aatatagatt ttttagaaaa atacagaatg cttcgtaata aatacaacat
    19981 tcctgctaag ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa atctccaaaa
    20041 attaattaat ataatagacc aaatccatga agaaggtttt caatgttcac tagatgactt
    20101 tggaagtgga tactcttcac tcaatatgtt aaaagaaata aaagttgaca cattaaaact
    20161 tgaccgtgct ttttttagct cacctaatgc agataatatt gctgaaaatc atgtaataga
    20221 atctatagtt aatttggcaa aaaaattaaa tatgaatagt atatcagaag gtgttgaaac
    20281 aatcttgcaa atggattatc ttaaaaaaat taattgtgat atgatacaag gatatgtttt
    20341 ttcaaaacct ctgcctcaag aaatatttga gaaaatgact tttggaaaaa aactagaaga
    20401 aaccagtatg gatagttccg tttaaataaa ataaaaaact tgtctctata aaataatttt
    20461 aatcatttta taaagacaag tttttttatt ttccaattta attttatttt ctaaatttct
    20521 tatactcggc ttgttttaaa ttcccttttt aagaataata atacgattat catagcaata
    20581 aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat taaattgaat tggaagtaca
    20641 atgattaatg gtataaataa aataaattgt cttaaaagag ttaaaaacat tgaatgttta
    20701 actttactta tacactgaaa atacacaggt cctacaatag aaatccccat tataggcaat
    20761 gtaattagat ttaggcttat acctcttact gctatatcca tcaaatctga atctttatta
    20821 aatataccta caaatacttc tgggaaaact tgtactacta aaaatccaaa tgacaatatg
    20881 acaactgaca ctatgataga taatattaaa gttttctttg cacgctcata ttgttttgca
    20941 ccataattgt atgatattat agtttgcata ccttgattca atccaaacac aggcatcata
    21001 aacatcaata aaatagatgt aagtgctgtc atagctccaa tagctaaatc tccaccataa
    21061 acctttagag aattatttgt aaacatgtga gttatactta tagctacttc catagcaaaa
    21121 ggtgtcattc ctattgcaaa taccttcttt actatatctt tattcaattt taagttacat
    21181 ttttttagtt ttaaattgga tttccctctt ataaaataat gtattaccca aaagaatact
    21241 actatttgac agagtactgt tgctatagct gctcctttta tacctaaatt aaatacaaat
    21301 ataaatatag ggtccaatat caggtttaat acacatccta caatcattgt ccttgctgct
    21361 aattttggat taccttctgc cctaattgca ttattaagag aaaatgcaac taaattaaat
    21421 atacttccaa ccaatatgac actcatatag tcttttgcat aaaatattga atctttactt
    21481 gcacccaaaa taaaaagtat atcttctaag aaaactaatc ctaatatagt tataattaaa
    21541 gagataatta tagataatgt caaagcatta cctaatatct tctctgcttc ttctctattt
    21601 ctttcaccta actttataga caatcttgtg ctagcaccta ctgataccaa cattccaaaa
    21661 gctattatta gagtaaatac tggcatagtt acaccaagac ctgcaatagc tatagagcca
    21721 ataccttcca tagaaccaat aaaagctcta tctactacat tataaagtga agttaccatc
    21781 attgctatta ttgcaggtat cgaataccta aataataaaa tccatatttt ttcatgtctt
    21841 aatgcttctt gattctccat gttgtcacca tttacaccat gtcataagtt atacaaattg
    21901 gtttatttgt tcttgggtct gttgctattt ctgcatcaat attaaataat tcttttagat
    21961 tatccttcgt cataacatca tatgcatcac cttcacaaac tattttacct ttcttaatac
    22021 ctatcatgtg gtcagcaaat cttgctgcat tattaagttc atgtattacc atgacaatag
    22081 ttcttccttg ttttttattt agttcatcta ataattttag tatctccaat tgatgtgcca
    22141 aatctaaata tgttgtaggc tcatccagta ataatatatc agtttcttgt gccaaagcca
    22201 tagctatcca tgccctttgt ctttgtcctc ctgataaatc ttccatattt ctatccttaa
    22261 actctgcaat tccagtaact tctaaactcc aatttacaat ctctctgtct tctttactag
    22321 aattaccaaa tccactttta tgaggaaatc ttccataaga aattaattct gaaacggtaa
    22381 gaccactagg agcttgaggg ctttgtggaa gtacagccat atcttttgct atttctttag
    22441 acttcttttt gtagatgtcc tctccattta tatatatttt cccactatta gggctaataa
    22501 ttctacctat agttttaagt attgtcgatt ttccacaacc attagcccct atgatggtag
    22561 ttatttttcc ttttggtata tttaaattta aattttttat aatatctgtg tttccatatg
    22621 atacatttag attcttagta tttatacagt tcataacaac accctttcct aatcatttac
    22681 caacattaaa taaataaagt acggcactcc tattatagaa actactatac ctactggtat
    22741 ttctataggt gctagaagat ttcttgatat agtatctgct attaaaagta ataatgttcc
    22801 tatcaatgct gatactggta ttaatctttt atgctttggt cctattattt ttctacctat
    22861 atgtggagct attaaaccta aaaatgaaat actacctgct actgctgtcg atacaccaga
    22921 taaaattact gcatatatta taagtcttct tctttgtttt tctacatcta caccaagact
    22981 tatagatact tcatcaccca aatttaaaac atctatatat ttagatttat aaatagttat
    23041 acccataaaa atcactataa atggaagcac agctattaca tatttccaac tggttcccca
    23101 aatgcttcct gatgtccatg ccataaccct attaaattct tgtgttgtaa atttcaattg
    23161 gaatatcgtc aaaattgaag taaaagctat gtttacacct ataccaataa gtagtaatct
    23221 tgacgaattt ataccacctt tccatgcaag agaatatata agaaatgctc caaataaagc
    23281 tcctataagt gcaacaatag gcattgtaaa tacagtcaag ttactcatac cttcatatac
    23341 attaccattc ataaggtata tataaactac tacaaacaat gcagcacctg aatttattcc
    23401 caaaattcca gaatcagcta aatcattctt tgtaactcct tgaagtatta cacctgctgt
    23461 tgataatgca gaaccaacca aaagtgctat tactattctt ggtaatctta atttaaatat
    23521 tgcaatttca tgattacgct caccctgccc taaaaaagtc tttataacat ccattggttc
    23581 tatagaaaat gagcccatat ttaaactcaa taaaaaagtg ataaaaataa gcaccattaa
    23641 aattgatata acaagtaatg ttttagaatt tatttttata atatttttca tagtacctat
    23701 ttctccccct ttctaacaag gtatataaat attggcactc ctataagtgc tgttatagaa
    23761 cctactggtg tctcatatgg aggatttatc atcctactta atatatctgt atatacaagc
    23821 aatacagctc ctaatactaa agagctcggt attatatact tataatctga acctactatt
    23881 cctttagcta tttgaggaac tataagacct ataaaaataa tattaccaga cactgacaca
    23941 gatgctccag tcattaatat tacaagtact atacatataa atctaacaaa gttagttttt
    24001 tgtcctaatc ctattgccac ttcttctcca agacttaata tagttattct tggagccata
    24061 ataatagcta aaataaatcc aataccacca gctacaaata gcaattttat accttcccac
    24121 ttcactccta caagccctcc tgatatccag aaacttaatt gttgagatag gttaaaatac
    24181 atagatattg ctgatgccaa agaaattagc aatgtaccaa gagcgactcc tgcaagagct
    24241 aactttacat tattaactct tcctcttgat ttagaactta taaaatatat aaataatcca
    24301 cttatacttg ctccaataaa tgcaaacatc attacactaa aacttccatg tataagttga
    24361 ggaagttttt tttgaagtac cagtgatatc gaaattgcga atgtagctcc ttgtgtaatt
    24421 cctataacag atggttcagc tataggattt ctcgttattc cttgcattat tgccccagaa
    24481 actgctaaaa atcctccaac aagtgcagct gctattgccc ttggaagtct aacatccttt
    24541 actattctca tatttattcc atcttccatt tttatcaaac tatctataac agttgataga
    24601 ttcatatctt ttgccccaat agttatggat aatacaattc caaatacaag tacaactata
    24661 cttactaata tcaacaaata agttatattt tttttactca gcttagtcaa ttatcctacc
    24721 tccttttccc ttttaatcgt cagtataata tcatctatta agtattttaa ttttaagatt
    24781 tattaaaaat aggtgtgaaa aatgaatatc attatctcac acctactaag ttaagaatta
    24841 ctttgttaat tcatttttaa ctgattctaa taatagctct tttccaattg gattgtatga
    24901 ttgactgaaa aatggagcag catcaagtat tgttacatta ccatctttta ctgctcttat
    24961 ttgagcccag atagcactat tttctaaatc ttttttatct gcttctgtag caattacaac
    25021 tatatggtct gcatctatat ctgttaaacc ttccatactt accattggta gtgttattcc
    25081 atcctgtttt ggcatattct taggtctagc taatttcata tcatcattga ttagagtacc
    25141 tattccacca tcactaaata ccataaattg acctgaactt gccaacactg gtaaataagt
    25201 tttttctcca ttcttttcta taacttcctt acctaacttt gtagcttttt catcatactt
    25261 ctgtagccaa cttttagctt cttcttcttt gtcaaataac tttgaaacat cagttaactt
    25321 gcttctccag tcatttgcat aatctttcat cataactaca ggagctattt cttttaattg
    25381 gtcatatatt ttttcttgtc tttgactcat tattattaaa tcaggattta cttctaatat
    25441 agcttccata tccatagtat ccatcattga gtgcccaact atctttacac ctttaagctc
    25501 ttctcttatg taacttggta atttatcagt ttcatatgag tctacatttg ctgttgcaac
    25561 aggcttataa cctgctaata atagttcttc actacttcct gatatatcaa ctattttttt
    25621 aggatttgat ggtatcttaa cctctccttt aacagattgt acaactcttg tctctgattt
    25681 atctttatcc tctgtattct tatctgaaca tgctgtaaca agtactaacg tagttataat
    25741 agatataaat attgcaagtg attttatttt tttcatgatt acttctccta cttgtttatt
    25801 atacttacat gatattgatt atcataagca catatttatt atagcatttt tctattttta
    25861 gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa tcttaattat atatagctta
    25921 gataaggttt tcttatatta aacagtattt tttttaagaa ttggacacaa atacaatgac
    25981 ttttcagtta aacaatgata aaatgcaatt tatagataat gaatttagta aggaggattc
    26041 aaaacatggt acatttagta tattgtgata atgcaggaaa aaaaggagaa aaggtcttag
    26101 ataaaatctt ggctggaaca aaaacaatgg ttattagagg tgctgctggt agaaaaatac
    26161 cacatagtag agtatttgaa gatgagttac tttattttat ggaaaaagga actgcaaaaa
    26221 tatctgcaaa agccactgtt aaaaaggtag aaaactatgt taaattaaca gaagatgaaa
    26281 tttctaaaat tttaaatgaa aatcaaccaa aattaaattt atctgaaaag caaaaagttc
    26341 gttggcataa aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa ataacacctc
    26401 ttgattttga ccatcaagga aatatggatg attggcttat tgttgaaaaa attgaagatg
    26461 tagttgttgg tactagtata ccttacaatt atgagaagtc taaattttaa agtatcaata
    26521 acataatact atattgctaa actctactta aataaaaaaa gagtgcattt aataattata
    26581 aaaattattt ttatacactc ttttttaata ataaatttaa attacttact ttctaatctt
    26641 tcatatacat ctataaattc ttctgctaat tgttgaaaat tcatagctgt cattaaatgg
    26701 tcttgtgcat gaataagtat aacacttatt tcagttttat taccattagc ttcattttgt
    26761 atcaattctg tttggaattt atgagcttta tgaattgatt ctttcgactt ttcaatacat
    26821 tctctagctt tatctatatt cccgctcttt gcttctttta ttgcttcaaa tgctagccct
    26881 ttcgcatcac ctgcaaatcc aattattcca aaagatatct ctatcatctt ctcatccatt
    26941 tattcgacct cctaaatatc tctatagctt gatatgacta atccattctt ttctaaaaat
    27001 tctttgactt ttttagatgt aagtatcttg tgctctttcg ttctatcaat agcataagat
    27061 gtagaattta aaatataatc atctaaaaaa gctgggtgag acataatatc cactacatca
    27121 tacttcatta tttcgtctat attttttata aaatattctt cacttacatt ttccttataa
    27181 aaactatcta ttaaaggaac tactttagaa tactctaaat tatattcaaa tccacctctt
    27241 attggaagat tatacttggt tacaatcttc tctattactg actttaaact tactaaagta
    27301 tggatatgat ggtgactatc taaatgagat atatctattc caagcccttt taccctatct
    27361 atttgagcac aaagttctct ataaatctca tcacaatcca ttttttcaat aatttcgtct
    27421 gtaatcctcc taataaaaaa accgtcttta tcaacaatag tttttaagtt agataataat
    27481 ggtctaccac aacttaaagt catatgaact ccacaattta aagttttatt ttctttaagt
    27541 aagcctactc catgttctac cccaggcata tttgccatca tagaagtaga cctaactata
    27601 ccattattat gagctgaaat tattccatag ttcacagctt cacaatatcc aaaatcatct
    27661 gcatttatta taattttagt cataccataa cctactttct atagacaact atctattaaa
    27721 tctaggaaga taatctttat gagcttccaa catttcgtct aatatagttt ttgctaagtc
    27781 atcagatgga atcaatgggt ttatacaaag tgccaaaaga gcagattcat aatcgccata
    27841 tacagctgct ttagctgcca atacttcaaa tgatttgatt tgacttacta atccatgcac
    27901 agaatctggt aaatatccta tacttattgg ttttgggcca ttttttgtta ttacactact
    27961 tacttctacc gcttgttcat ctttaaaatc tcttatagaa cccttattta atgtattaac
    28021 tacttggata tcttttttgt cgttgtatat agaacttatt aaattacaag cagcatcact
    28081 atagtaagcc cctcctcttt tttcaagttg aggtggtttt acatctaatt tttcatcctt
    28141 atataattca aataattgtt cttccaattc tttaactact tgacctctag cttttccctc
    28201 tttaaactct ctcaactcat cctcaagcat ttctttagtt ttatagtagt atctatggta
    28261 tggacaaggt attgcaccca aagattttac aaattcagca ttaaaatcaa ttgcctttat
    28321 attttgcata cttatatctg ctttgacaaa tttatcaatt gcttcttttg taacatcttc
    28381 tccatctaaa gatacattta gaccataaac catatgatta agacctgcaa aatccatact
    28441 tattctgtca ctttctacat taaatagttt ggcaacatca ttttttaaat gaattggcac
    28501 gttgcataga cctatatatc ttctaaaatt agtatatttg aatacagcct ctgttattat
    28561 tcctgttgga tttgtaaaat tcaccatcca tgcatttgga cataattctt ccacatcttt
    28621 tattatatca aatataactg gtattgtgcg aagggcttta aataaacctc cagcaccatt
    28681 agtttcctgc cccataactc catgagataa aggaatagtt tcatctttta ttctcgcatc
    28741 taaaagacct actctaagtt gtgttgtcac aaagtctgca tctcttagag cttctcttct
    28801 atctaaagta agatttattt tcatatctac tccagctttt tttaccatcc tcttagctag
    28861 atttccaact atctctaatt tttcttttcc agcttctata tctacaagcc ataattcctt
    28921 tacaggtaac tcttcatatc tatttataaa accctctaca agctcaggag tataacttga
    28981 acctcctcca attgtaacta tttttagtcc ctttttcatc tcattatacc atccttacct
    29041 ataatttaat atactctaca aaaccctata catttattta ccgaaataaa actacctagt
    29101 tgttaatttt caatttatta gcttccattt tttcagacgc tatgacaaat ggaatatata
    29161 ttaatattga taatattaaa ttaaatagtg ctaaagctcc gccagtccag tcacctgtag
    29221 ccattgctcc acctaatata ggaggtgtta cccaaggtat tttaggtaaa attactggtg
    29281 ctactaatcc aaaatctatt gctaagtatg atacagcact acatattact ggtgcaagta
    29341 caaatggaat cataaatata ggatttaaaa ctattggaag tccaaatatc aatggttctc
    29401 ctatgttaaa tattgcaggt ggaccaccta atactatcat ctctttacgt cttcttccag
    29461 ctattaataa agctactatt aatcccaata ccattccaga accacctaaa tatacaaatg
    29521 catcaaagaa tgcacctgca agtacatggt atccttccgt tgctcctgct tgagccaagg
    29581 cagcattttc tccacctaat ttcataagta tagtttctgt aaaaggaagt gctatatttg
    29641 caccatgaag tccaacagtc caaagtatat gaattataaa tgcaataagc attgtagaac
    29701 ctagactatc agctacattt gatagcggtg ctcctaaata agtatttaaa atatctgtta
    29761 agaaacttcc attagaaagc atttttataa atattcctat tacagtaaat atcattatag
    29821 ttaacattcc tggtattaac tttgcaaatg atcttgaaac tgctggtgga actccatctg
    29881 gcatttttat agttaatttc tttaccttag ataatcttac aaatatctca gttgataata
    29941 atcctattaa tatagaagag aataaagctg ctgtacctaa gtaggtttgt tgtatcattc
    30001 cccatccttc aactactgta gttcctccct caggaactat tttaccaatt tgtggagcca
    30061 ttataaaaaa gatagataaa gctataagcc cagattgtaa cccatcactc tcataagatt
    30121 ttgctagaaa ataagcaaca cctattacta aaaatactgc cattgtacct attgctcccc
    30181 accaaaggtc gcctccaaat gtggtccagt tttctccaaa tatacttgcc atcaaatttt
    30241 tgtatgcttc caatggtaag ttatttatca atgttcccaa tgcacctacc atagttatag
    30301 gtatcattac tataaatgca tctcttactg ctactaagtg cctttgagct cctattttag
    30361 cagctacagg cactatatat ttatccataa atgacatgaa tttttccatt ttacatcctc
    30421 cagtacaact ttattttgaa ctatttaact ctacagctct atctaacaca gcttttccat
    30481 tacatcttcc ataatccatc atatttataa cttctactgg aatattatat ggttttgcca
    30541 tttcagttgc ttttcctaat acatatctaa cttgaggccc taaaagtaag acatcacaat
    30601 tttctatttc attttttaag ttcacttctg atatagccca tatttttgct tctataccgt
    30661 tttcctttgc tgcatcctcc atttttgtaa ctaataaact agttgacatt cctgctgaac
    30721 aagctaacat tatttttatc attttttccc ctccaaatta gattcattaa atgtttttta
    30781 agtcataagt tttttaaact atacatttaa aatatgcttt tctattaatt ttttcttaat
    30841 gtatatatct tatttttaaa tgtatataca tttaattctt gatattatta tataccaaat
    30901 gtatatacat ttcaagaaaa ctttttcatt ttatttaaat tttattcgac aacagcattc
    30961 aaagttaatg tatatccata gtgatatgtg tgtgagtact caaaaatctc actattttgt
    31021 aacttacaaa tttggcttat atataaaagt ggctctggtt cttctaattt tagattatcc
    31081 atacactctt cattcgatat tacagcttgt acttctctct ctgacatggc tatcttgtat
    31141 ccacacactt cttcaacaaa attataaaga gaattttcac aatgagtctt atttaaattt
    31201 ggaaataact taactggcat ataagtaatc atatatgaaa ctcttgcatc attggctaat
    31261 cttactcttt ttatttccca aacattttct tctaaactta tattcaactt acttgataaa
    31321 ttttcatctg cctgaactat ttcaagagat accaatatat tagatacttt atagccttta
    31381 ctttccatct ctttagtaaa tccacttaca gacgatatat tatcatatat attatttgca
    31441 ataactatac tacctacacc tctttgtcta gttatatagc cttctttaac tagattatta
    31501 agtgcagacc ttacagtcat tctagttaca ttaaattctt cactcagctg tttttcagaa
    31561 gggattaaat ccccttcttt caaactgtct gaatttatta actctctaac atgattttct
    31621 ataactttat aaattggctc tttcatttaa ttcactcctt attaaatctt ttatataata
    31681 agataatttt atatacaaac aaagaaaatg tctatacatt taagataatt taaattttat
    31741 tcagaaatct tgtacaactt tccgagtttt aataaagata taaatagact tatcattaat
    31801 atacctttta atatacttga taaggttata ctccaccata caccatttag acccaacatt
    31861 tctggtctag atataaagta agccaatggt actcttgcac cagtcagtat agtaattata
    31921 atagaaggta tataggttct ccctaatcct ttgaaagctc ctgttgttat tatttctaaa
    31981 cacataaaaa attgagaata gcctaatatt ttaagataaa ttgctccttt ttctattgcc
    32041 tcactttcat ctataaaaat agaaaatata ctctctcctg caaatactaa tactaaagta
    32101 gttattatac caagtacaga agatacagtt atagctattt tgcaaccttt atttattcta
    32161 gaatacttct tggcaccata attttgtcca acaaaactgc ctaatgcaac tgatagccct
    32221 tcagccgtca tccaggaaat tgcttctatt tgtgagccta ctttttgtac tgcaacagcc
    32281 acaggccccc atgatgctac tattactcct aatagcattg agaatactgt aaacatacca
    32341 ctttgtattg ctataggtaa tcccaattta tacagaactt tataatattt agtctctatt
    32401 tctttaaata gtcttaattt aaaatattct tctttacttt taactattat atatataaaa
    32461 catgtagtaa ctactatctg agcaaataca gttgcaatag cagctcctgc aacaccaagt
    32521 tttggagcaa atccccatcc aaaaattaat atagggtcaa gtataatatt agttattaat
    32581 cctactgtat taatacgaaa aggagtttta ctatttccaa gaccattgaa aattgcagta
    32641 aatactggat taataaaata aaataccata cctagagcta ctattatcaa atactgtcta
    32701 gacatggtta taacttctaa gtctcctaat ctaaaaaaac ctatcaattg tttattaagt
    32761 actattaaag aaattgtata tattattgct agtataatat ttatttctat tgctgacttc
    32821 atatatgatt tagtagtgct tatattattt gcaccaatac tttgtgctac ttttatctct
    32881 ccacctactt ttgaaatcat aataaatgcc attgcaagcc atggaaaaaa accagctgtt
    32941 ccaactgcag ctactgcttt acttccggct ttaccaaccc atatcatatc tatcatgtta
    33001 taccccatct gtataaatga agtccccatt attggaagtg atagcttaag taatttttct
    33061 gtaattcttc cttctgttaa attaagtctg tctttcattt tccacctctt ttatattaaa
    33121 tttatttgct tatatagcta gattaatcat tttatttgat tgagttcact atccttcaat
    33181 ttatattatc atatctcagg attgcatctc aatcatactt atgtttaaga tacagtattt
    33241 tacctatcta tgataaaatt aatacttatg aatttaaatc tagccatttc atcaatacat
    33301 attctaaccc ttaacttaac ttcaattaag tatacatatc gtaaccaatt agaatatttt
    33361 atacggtatc ttccattaat aaacaaccaa tatcaatgat ttatatataa ttttatttat
    33421 acataaaata gtatactata ataataaaaa aactatattt gaatgcttta agcatgttta
    33481 ctaattgtaa tttccacaca aatttgataa tatatgaata tattatggtg aaatttatag
    33541 atatatatta attatgggga gattgtggga ggttcttttc acaagacatt taagctatga
    33601 aaataaataa aaaattcaat agagttgata tgcaggtttc aatactcact gcaattattg
    33661 taataatatc ttcctcatgt gtatattttt ttaattatac actaacttat aagagcatga
    33721 tatactcttt aagtgagagg tcaaaacata tctatgaata tgtagaaaga aatattgata
    33781 aaaatacctt tactgaaatt aattctaaag aagaccaatc aaaaaagtct tataaaagtt
    33841 ctaaaatcct tttagaatct gtaaaaaata ctactggagt catgtatcta tatactgcta
    33901 aaaaaacaaa agatggatca ctagtatatg ttgttgatgg tttaaattca tctaggagtg
    33961 attttagaag tgctggtgat ttgatagaaa aagagatttg gggagacctt aacaaagcat
    34021 tagataatca tatattgtta cctaaaaaga ttaaatcaac atcttgggga tacatattta
    34081 tatcgtattt tcctattcac aatgatgttg gtaaggttgt tggagtaata ggaattgaat
    34141 ttgaagcaaa acatcaatac aatacattta aatttataag tatagttaca ccaataatag
    34201 cattattaac ttgtctaata tcagcattga ttgcagtgat tctatttaaa agaatatcta
    34261 atcctacata tactgatttt gctaatacag attatttaac aggcctaaaa aatagaaatg
    34321 cttttgaaat agatatgaat aacttaaaca attcaaatct aaagaatcta atatctatca
    34381 tatcaataga tttagacggt ttaaaaaaga ttaatgataa atttggccat cagacaggag
    34441 acttatatat taagcaagca agtaaaatta tacaatatgt aataaataaa aaatatatag
    34501 tttatcgtat tggaggtgat gaatacataa ttattctaaa aaatttatct cacaaagaaa
    34561 taaataatat tataaataat attaatttaa aaatagttga agaaaactca actaatgaat
    34621 taaaattatc aatgtctatt ggatatgcta tttttgatga aaaagtagat gtttctttat
    34681 ttgatacata tcgtcgtgca gattctcaaa tgtatgataa caaaaggaaa attaataaac
    34741 aatatcaata ataaatttaa aaagtctatt ctttaaataa taaatcacaa gaatagactt
    34801 ttttaaattt gtgtttttat aagataaatt atttaattaa agtttgttct tttttctaaa
    34861 cgattcttta taaattcata tattactttc actaatatat ttgtgactaa tattaacaca
    34921 gctagtgctg ctgcctgtgc agtactcccc ttatcatcta gttgtacaat tgatattgca
    34981 gctacttttg aacttgcagt atatataaaa actaatgctg atattgtcac catggagttt
    35041 aggaaaaagt acataaagtt ctctaaaact gctggcaaac aaagtggtat agttacgttc
    35101 ttaagaattg atgcactgtt tattcccatt gatttagcta ccatatccaa ttctttatct
    35161 actttcttaa tagcagtagt agctgttata aatgctactg aaaagaaatg aactatatta
    35221 catataacca ttataagtaa tgttccatat aatccattaa acaaattctt aatgtatatt
    35281 gtatccataa aattaaactc taatttatta aaaaataata cataagctat acctaaaact
    35341 aatcctggca atgccatagg caatgtactt aaaaaatacc caaatgattt taaatatgga
    35401 gctttttctg ttttttcatt caaatatgca aataagaaaa caaagctcgt ccctagaata
    35461 gctgtcaata gtgacatttt tattgaattt ataaatggtt taatcccatc aatactgttt
    35521 ttcatagaaa aatgttccag tgtcagtctt aaatcatatg gccaaactga tattaatgaa
    35581 gataagatta cagttaacat tacagctatt ataccaaatg caataattcc tacaacccct
    35641 cctaaaatac tatctcttaa cttattagtt ttaaccttat atggaattga ctttgaagtt
    35701 atatatgaat tttgattttt ttcaattttt ttagttacta taaacgatat tattgcaggt
    35761 attaaaagta atattgatac agttgctccc attggcatat tctgttgccc tataatttgc
    35821 ttatatacat ctattgataa cacattaaac tctcctccta tcacctttgg agctccaaaa
    35881 tctgtaaaac ataatgtaaa cgatacgaat gcagaactta ttaaagagta ttttatactt
    35941 gggattgtta tgcttataaa ttctcttatc ttgcttatcc ccatagtatt actagcttca
    36001 tatagtctat aatcggttga acttagagat atataaagta tttgaaatga ttgaggaaat
    36061 acatatataa tttgtgccaa tattattccc actgctccat atattggaac atgcactcca
    36121 agtaaattag ttaccaaacc ttgatttcca aataaatata ctagacctat tgcatgcatc
    36181 attgttggag caaacatagg aaacatagct aatgatttaa aaaaaccttt aaatttaatc
    36241 tttgttctag ttaatgcata tgcatatact aatgcaagtg ttagtgatat tacagttgat
    36301 ataactgata caaatactgt attttttaga gaatttaata gagattcaga agccaagtat
    36361 tcttgaaagt tttgtaatcc tacaaagact ccatctttat ctaaaaaagc ctgtacaaat
    36421 agatatatta taggtgctaa taaaaatatt actaaagaca taactaatac tgtaagcata
    36481 cattttttta tatctaaatt catttttgat ttaagactta tacttttaga acttatagca
    36541 ttattattca tacgcttatg ctcctttaac ttttggatac tttaaatagt atttttcgtc
    36601 tatacttatt tttacaaggt caccttctac taaatttaaa ttaacccact cttttataga
    36661 aacatcactt attatgaaat tttcatttaa tttatctttt atttcaactc tcaataaatt
    36721 ccctctaaac tctatatttg atattatccc ttggtagttt tcttttgtcg atttctctat
    36781 ttgaacatat tctggtctta cagttaatat agaatctcca ttatcaaaac agtttgtatc
    36841 tcctataaat tgagctgtaa ataggttttg aggattttgg tatatttctt ctggagtccc
    36901 tatttgcatt atatctcctc catttagaat agctatttta tctgccatag ttatagcttc
    36961 ttcttggtca tgtgttacca ttattgtcgt tatattaagt tctttttgta atcttttaat
    37021 gtccattcta agcttgtgtc taacctttgc atctaatgcg gacattggtt catctaaaag
    37081 taaaaacttt ggttcaagag ctaatgctct tgctattgca atcctctgtt gttgtccacc
    37141 tgataatgct ttagggtatt tatgagcttc attagtcaat ccaactgttt caagtacttc
    37201 cttaacttta ttatctattt tttcttttga aacttttctc tcttttagtg gaaaagctat
    37261 attgttataa gcagtcatat tcggaaataa tgcatatgat tgaaatacta taccaaaacc
    37321 tctctttgat ggctctaaat ttgtaatatc tttatcctgg agaattatag ttccactatt
    37381 tacatcttca agacctgcta ttattctaag taatgtagtt ttaccacatc cactaggtcc
    37441 gagtaaacat agaaattccc cttcttcaat atccaaacta atattattta aaaccctttt
    37501 ttggtcataa cttttaaaca cattatttat ttttaagtaa ctcatatctt atctctcctt
    37561 atatagttat ttttttattt taaattttat atcctacttt ttcttctaaa attctttcat
    37621 ctaaattatt aatttaaacc ttacttactt tcagattttg aaccgtattt tgattcccaa
    37681 gttttaagta ctgtacttct gtttttggca gcagagttta aatcaacttc ttttataagc
    37741 tgttcaaatg gttgtttaga ataacctttt ggaactggat tatctgtctt tatggaagta
    37801 actgctactt catgtgcata tctatccata gctgaatcac ttattgccca atctaaaaat
    37861 agctttgctt ctttttttat attatctttt ttaactaagg catttgcttc taagtcccaa
    37921 cctgaacctt cctttggaaa tacaacctct ataggttctc cactagataa ttgttgtgta
    37981 gctctatatc caagtgtaat acctattgga tactctccag agccagctaa cttcgcaggt
    38041 tttgaacccg agtgtgtata tgtggctata tttttatgta atttatccat gtaattgtaa
    38101 gcatcttttt cacccatcat ttgaataagc cctgatactg taagataccc cgttccagat
    38161 gatgctggat ttggcattgt tataagtcct ttatattctg gttttattaa atcttcataa
    38221 ctttttggaa tatctatacc tagtttctta agttccttat aattaactac tattgctgtc
    38281 tctgggacac tcattcctac ccaagataca tcatcttcag tatctttaaa ttttttatca
    38341 actttatcat aatttttagg cttatatgct tcaagtgcac catctttttt tagctctatc
    38401 atgttcattg ctgataatcc ccatataata tctgcttgtg ggttatcttt ttctgctaat
    38461 agttttgaag ctattacacc atgagaatcc ataactatct ctacatctat attgggatac
    38521 tcttctctaa aaccttccat ataataagct atctgctctt cctctagtgc tgcatatatt
    38581 gtaagtttat tatttccttt tccttgagta gtttgtggtg ttgttgatat gcaacttgtc
    38641 ataaatgtaa tcactacaat gcacagacaa gtaaatataa attctttcat atcttccccc
    38701 ctttgtgttt tttccttttt ttatttgttt tttattgttt ttgcttatgt ttatataata
    38761 cacctaattt gttaatataa agttaagtgt aaattaacag ttcgttaatt tttagaaatt
    38821 tttgttaaat atattatttt tagttaaata cagtctgttt ttaaacaata tattaatttt
    38881 tatagtatct acattttaat ttaaacataa aaaataagtc taacctctat aaagtagaat
    38941 gttagactta tttttataag tatttaatta tacaaccaaa tcattttttt tcttttcttc
    39001 aagtgctttt ttaagcacca tatccttaac cttcataagt ctagtagtac ttgctctctc
    39061 attttcttca agcttcatag ctatatactt aatagtctca atgtaatttg gtatcattac
    39121 attttcaaga gcattaacac gtctacgtgt cttttctatc tcttgtgcaa gaagctgtgc
    39181 acttttttca ctttctgcaa gtcttaaaag tggttgcata gcatctgaga aagcttccat
    39241 agcagaatct aattcacctg atgtaaaagc aagaccataa ggataaatat cactttgatt
    39301 gttttcagtt ttaaagtcaa acacaggaac atcaacactc attatatttc tcgtagaaac
    39361 atctactgaa actgattgct ttggcatcat aagtgctgaa cccagatact cctgactcat
    39421 aactgctctt gcgattataa agtttttata agcagtatca agagcatttt ctgcttcttc
    39481 acgcaatcgc ttattttctc ttacaatctc aagaaattgt ttcataagtt catcaagttt
    39541 atctttaaga agcttatgac ctcttgtagc agttttaagg agtttcttaa gtctagtcat
    39601 ttccatacgt gttggattta tatttaatct agccacagat atcactcctt aggcatatat
    39661 ttttcaaaat attcatcacg gatacgttta agttcacttt ttggtaacat actaagaagc
    39721 ttccatccga tttctaatgt ctgctcaata gttctatcag ttctaaatcc ttgtgaaaca
    39781 tattctttct caaattcatc tgcaaactta gcatacatta aatcaatctc agaaagtgca
    39841 gattcaccaa gtatagccat aagttctttt gcatccttac ctcttgaata agctgcaaaa
    39901 agctggttca ttgtatctgc atggtcttca cgagtcttac ctttaccaat acctttatct
    39961 tttagacgag aaagtgatgg taaaacatct ataggaggct gtatatcttt tttataaagt
    40021 tcacggctaa gtataatttg cccttctgta atgtatcctg taaggtcagg aattggatgt
    40081 gtcttatcat cctcaggcat tgtaagtatt ggaatcatag ttatagaccc ttcatgccct
    40141 ttcatttttc ctgctctttc atacatagta gcaaggtcag tataaagata tcctggatat
    40201 ccacgacgtc ctggaacttc ttttttagct gctgaaactt cacgcaatgc ctctgcatag
    40261 tttgtaatat ctgttattat aacaagtaca tgcatatctt gctcgaaagc aaggtattct
    40321 gctgctgtaa gtgccattct aggtgtagat acacgctcta cagctgggtc atttgcaagg
    40381 tttacaaaca ctacagaacg gtcgattgca ccagttgctt taaaatcgct tataaaaaat
    40441 tcagcatctt caaaagttat accaactgca gcaaatacaa cggcgaactt agaatcagtt
    40501 ccacgtactt ttgcctgtct tgcaatctga actgcaagat tggcatgtgg caaaccagac
    40561 cctgagaata taggaagttt ttgccctcta accaatgtat taagtccatc tatagcagag
    40621 actccagttt gtataaactc ggccggataa tcacgtgctg ctggatttat tggagctcca
    40681 tttatatcaa gtcttttatc tggtataatt tctggacctc catcaatagg tctacccatt
    40741 ccactaaaaa cacgacctaa tatatctggt gaaactccaa agtcaagact ctttcctaaa
    40801 aaacgaactt tactttcagc aaggtttata cctgttgagc tttcgaaaag ctgaacaagt
    40861 gcagtatctc catttacctc tagaactttg caacggcgaa tttctccatt tgaaagttca
    40921 atttctccaa gttcatcaaa ttttacacct tctacgccgt ttattaacat aagaggaccg
    40981 gctacctctt gtattgactt atattccttt atcatagttc ttcagccccc ctctttttaa
    41041 taaggtctgc aagctcgttg tctatttcat cttcaattga atcgtaagct gcatctatat
    41101 cgccttcttc tacatactta gcacgaccaa tcttttcaag aactgaaagc ttgataatat
    41161 cgtttatagt aacattttgc ttgatagcat cttgtgctga cttatagaat tttaatataa
    41221 gtcccatcat tctatattgt ttatgaagtg atgtataagt atcaacttca tggaaagagt
    41281 tttgatgaag gaagtcttct cttatagaac gagtaacctc tagtatcaat ctatctccat
    41341 ctgaaagtga atcaacacca accaattgaa ctatttcttg aagttcagat tcaacttgta
    41401 aaagtttcat agcttgagct ctttgagctg agaaattatc atttacattt ttatctgccc
    41461 atacatcaac tttttcctga tagagtgaat aactagtcaa ccagttaatt gctgggaaat
    41521 gacgtttata tgctaaagat gcatcaagac cccagaatac ttttactata cgaagcgttg
    41581 cttggctgac aggttctgat gtatcaccac caggaggaga aactgcacca attgctgtaa
    41641 gtgttccttc tctttcatcc atacctagac aatttacctt tcctgctctc tcatagaatt
    41701 gagcaagacg tgaacctaag tatgcaggat aaccttcttc accaggcatc tcctctaaac
    41761 gaccactcat ctctctaaga gcctcagccc atcttgatgt agagtctgcc ataagtgcaa
    41821 cactatatcc catatctcta aaatattcag ctattgtaat accagtgtat atagaagctt
    41881 cacgtgcagc aaccggcata tctgatgtat ttgctataag cacagttctt tgcataagtg
    41941 actcgcctgt tcttgggtct tttaattcag gaaattcaag aagaacatct gtcatttcat
    42001 ttccacgctc gccacagcct atatatacaa ctatatctgc atctgcccat ttagcaagct
    42061 gatgctgtgt aacagttttt ccacttccaa aaggtcccgg tatagctgct accccacctt
    42121 ttgtgattgg gaaaaatgta tctataactc tttgacctgt aacaagaagt gaatctggtg
    42181 ttttcttttc tctatatggt ctttcttttc taactggcca tttttgcatc atagttacag
    42241 gtatatcatt acctttttca tctgtaataa cacaaactgt atcttcaaca gtaaattcac
    42301 cagaatatat ttcttttata gtacccttaa ctccataagg aaccataatt ttacattcta
    42361 ctactgctgt ttcttgaacg ccaccaatta tatctcctgc tacaacctta tcaccaacag
    42421 attttttagg tttaaattcc cacttagttg ttctatcaag tgaagatact tcaacacctt
    42481 tagttatatt agtacctgaa atatcataca ttttttcaag tggacgttga ataccatcat
    42541 aaatagttga aataagacct ggtccaagtt caacactcat aggcattcca agagaaacta
    42601 cttcttcccc tggtcctaga ccggaagttt cctcataaac ctgtatagaa gctttatctc
    42661 catgtatttc tataatctcg cctataagat gtttatctga aactcttacg acgtcaaaca
    42721 tattcgcatc tctcatgccc tccgctacaa caagcggtcc tgacaccttt acaattgtgc
    42781 ctgttttcat attctctcac ctgcaatcat atactttatc aaaatttaaa tattccctcc
    42841 tatattttat tagtacatat atttagcacc aataaaattt gagattgtct tttattcaac
    42901 tttcatctat tctttaaata atatatctgc acctatagca cgtttactag attctcttac
    42961 ttcattcata cctaatccca tacttcctcc aatacctgga ataactataa tggcaggaag
    43021 ttggtaatct ttgtattttg ctattgattc ttttgcaagc aatgatgcct gttctgtaat
    43081 ataaattatc gcatattcat cttcaaccaa cttatgtata ctttttttaa tttcgtcact
    43141 atcataggct gggaagatat ctattccaag agctaaaaat cccatgatgc tatctttatc
    43201 tccaactact cctaccttat acataagcat cccttagcct ttcttttata gtatctgcgt
    43261 ctatattatt aagcttacta gtcaaaataa ttctaattgt ttttatctct gattctttag
    43321 catataaata tgcaattagt ggttctaatg taagaggctt aaattttgct cctctaacat
    43381 attccataag atagtcatca caaagttttt caaagacagt aaaaccacta tccatgccat
    43441 ttttacagac atcagagtaa gaagtagatt taaaacatga tgctggagta tctgaggaaa
    43501 aagcttctaa gaatttttcc ttatcaagtg agccacctga aacaaacaca ttcataaaca
    43561 tatcaaaatc tttcttcata tttttgactc ttataaaact ctttagattt gttaaatcac
    43621 aaactttcat aacataatct attacaaatt tgttttggct ttctttagca gtttctttca
    43681 tactggtaaa ggttgcttta tctagaacaa tatcaaccat ctggccattt tgtgtcttat
    43741 tataaacatc aaatgcctct aatatagctg atgacattat cttaggtaaa tcactaaaac
    43801 tacgatttgc taaagattct ctaagcttaa taagaggaat tgtacctcca tcaattaaat
    43861 atttctcacc atctacacca gaaatttctt ctttaatcag cacttttaaa ttgtgatagt
    43921 catttttata aagaaataca tcaacaaatt tttcttcagg agctagactt ttaagaattt
    43981 catatgtctt tgataattct tgagtcaaaa tattctcaaa attatgtata ttattatttg
    44041 atgtatcagc ataacctgcc tcagctataa ttcttaaaga gtcttcaggg cttttggctt
    44101 ctgccatttg aataaacatt tgtctgttaa gaagctgttt ttcaagcact cttattcgtg
    44161 caacggcata gggataagtt ttttcttctg ccatttttta acccctcctt aattaaacaa
    44221 aatttctgct gcaatttgtt caatatattc atgctctact gcaattatgg cttcaaatga
    44281 ataattgtac tcaatgtcac cttttttaac tatgaaacct ccagtaatat ctctagtttc
    44341 atcagacact ttaatgcctt tttttgatag cacatctttt aatgtttttt tatctttttt
    44401 agataatact atctctgaat catctgtaca atttgatttt tcaatcatgt ttagtataat
    44461 ctcttcatat tcattatctt taagatttaa aagtttgttt ttagcttcta aaattacctc
    44521 ttctaaaatc tcttgttttt ttgataatat ttgcttttta gcctccatat aagctcctga
    44581 tatttcctta gaagccgctt tttctgcttc tgcctctgca agctttgtat aggaagccat
    44641 ttccttttca gctttttcat tagctgaatt tacttttaaa tctgcttcac ttttagcttt
    44701 atccaatatt tcttgtgctt cttgtttggc atcagctata attctgtcaa tcattttttg
    44761 ttcattaccc ataggagtgc ttcctccttt tcattttttt tacttatgaa ttataattta
    44821 taattaacca attgcaattc cattgtaagc taagaaagaa acaagtagtc ctaaaagagc
    44881 atatgtttca actattgctg caaatgttat agctttacca agttcttcag gtctttttgc
    44941 aactattcca actcctgctg ctgctgcttt accttgtgct atacctgata ctaaaccaac
    45001 taaaccaatt ggaagtcctg ccataagaag ttgtaaacct tgagctgatg ttggtattgc
    45061 atctccacca ataatccctg cttttgataa aataaggaaa gctatgataa atccatacag
    45121 accttgtgta ccaggaagaa gttgtaaaac tagaagttga ccgaatttac tagggtcttc
    45181 tgttacaaca cctgctgcgg cttgacctgc tattgaagta cctttagcag aacccatacc
    45241 tgcaaataat gctgctatag cagctcctga tattgccaaa aattgaccat ttcctaacgc
    45301 gtttaaaaat tccatattta catctccttt ttattaatgc ttgtatattt tgttttatac
    45361 ttaaacggaa caaaaggaac tcctccgcct tcataaaact tattgaaaaa ttctacatac
    45421 tgtaacctac ttgtatgaac atatgcacct agtgcattta taagaaggtt tattgtgtgt
    45481 cctacaacac caattacaac tgcaagtatc ggaccagcta tagctcctaa aaggttgata
    45541 acttgtgcta taactcctgt tgttaaacaa agcgccataa ttcttgtata agataatata
    45601 tcagcaaaat aacttgttat accatagaga cttgaaaatc ctttaaaaag cttaacagga
    45661 atacctttaa agcttcttcc ttgagtaagt acaagaccta tagcacctat aattgctaaa
    45721 tattttccta cttctgaaaa tacacctatg tcccctgcaa caaaaggtat tataagtaga
    45781 catacacctg ttatacacaa ataccaaaaa cctatgtcaa agattgcatc tattaccttt
    45841 ccacttctta ttaatgtata tgcttttatt cccaaaccta catatatatg gataataccg
    45901 aacagtaacg acattcccat aagtgtcata acatcctcta aaggattaat taatgctttt
    45961 ataggaatta aatcccccaa aatacccccg aatataagac cccatacagt agttgaaact
    46021 ccacagatac caataagttt aattaaattt ccttcacctt tttgtatctt taatttatat
    46081 acaacaaatc cacaaactac tgcaattatt aacccatatg cggcgtcact tagcatcatt
    46141 ccaaagaata caacaaagaa gaaagttaaa attgtatttg ggtcaatatc ttttgtactt
    46201 ggatagctgt acatgttagt tacactttca aaaggtgtta ctaatttatt gttttcaagc
    46261 agaattggga agccttcttc tttacttcct tcttcagtct gaacacaaca atcaaacttt
    46321 tctgtaattt ctttaattaa ttcatctgcc cttttgcttg gtaaccaacc attaagacag
    46381 aatgttgttt ttgttttgac aagtctctct ataatctttt tttggtctct ttctatagtg
    46441 taaaaatcat ataggttttc aagcatgtgt atactatctg catgtctttt aatttcctct
    46501 gttttgtcct tagaaacgtt ttcctgtcta attatcattt cattgtattt cttgatagct
    46561 tgtaacggtg taccttcttc atttggtaat gaaaccactg aaaaattaaa ttcttttaaa
    46621 acttctaaaa catcatcaaa agtttctttt aaagtaatta aataaacata gttcattaac
    46681 ttgtcagaat tgacaatccc aataacactt tctggtattt catcttctaa ttttactctt
    46741 aaagcatcaa tgtttgcgct tatgggcaca gttccaagta tagtttttgt atacttagtt
    46801 tccatatttt caataggaat atcaaagttc acccatggtt ctaacatact tttattgttg
    46861 ttaagtaagt ttatgttaga ccttattgta cctatctcct tgtgcaaatt gtttaactcc
    46921 agagcttcag cataaacttg cccttctttt tcactatcaa ttttgttaaa ctctcttttt
    46981 ggtgcaagta gggatttttt ttgtttcact agtgaagaaa caatattaat tgcttggttt
    47041 acatgagaca ttttctgctc tagcattata acttcagatt cttcactatc ttttacaaga
    47101 taatttttta attcctcctc ctcagtaaga aaggcactat catcaatctg aacaaaccca
    47161 cggttcataa gtagctttaa aatctggctt ttttgagctt ctaagccaac aatagatatt
    47221 ttgttcatat taactattgc catattatct cactaccttt ccaataaccg cttcaatagc
    47281 cttacttttt ctttcttcag cagttttttc aattttcaga cattcacttt tagcattagc
    47341 taagatttta tcaacctctg ctctactgtc ggtgactgcc ttctcaataa gagtttcttc
    47401 cataagcttt gcttgttttt ttacatcagc aaatatgctt tcacatttgt ttacagcatc
    47461 agctctgata gactctgcat cagtaacagc ttttactttg atactgtctg ctgtctgctc
    47521 tgcctcaaca atgcttttta ttatttcaaa agccatccaa atcacctctc ttaccttttt
    47581 actatgtata taaccaatta gattttttta ttctaattag attactacct cattgttaaa
    47641 gtataaacag catttttata taataattaa tatttttcaa cgtttatttc ttaataaaac
    47701 atattttaga atagtacttg cctagattta catccttttt tgacttactt ttagactatg
    47761 acatgagtat ggtttttaaa taccattatt ttcaataatt ataatttaaa gcgatatagt
    47821 ttgtcaatag cgttattttg ttttaaaatt aacgttatgt cgacatttta aatttttatt
    47881 ttattttttt tataatttta atttaattta ttacatataa aagtttttta tattcttttt
    47941 caacctttaa ccattgaagt ttattgtttt ttttgaattt ttagaaatat ctcgcactat
    48001 tttatctaac ttttataaat catcaagcta tatcgatttt tgtcacctta tttttaatct
    48061 aattttttta tatctagtta gaaataaatc aatattttta aagattgata aaacttacac
    48121 ctagttctaa tagttttatt ttaaggcaat gtattaatct taagtagata atatcattag
    48181 ttactctata tattcgacaa cattataaaa aaatcctcgt accaatcact ccaatcttca
    48241 ataggatatg ctgctcctcc atttacactt ttcagtagat tggatgtata ttcttctccc
    48301 atagaaaaaa gttcccctac tgtcatatca taacctctag atgtaacaac cttacaaaat
    48361 gaatccataa catcacgttg ttctcttgtt tttataagtt cctttctaaa ttcatcatca
    48421 ttcttagctt cttctaaaat tttagttaat ttttcattca ttatgtattt ctccttttaa
    48481 gtagtctttc tgtaaacttc aataagcttt atataaaaag gaagccatcc taaataaact
    48541 aagatgactt cgcttaactg ttagcaacac atttactagt ataaaataat tggtgtacct
    48601 ggttctatat tttcatatat tttctttgcc ttagaaaaag gtgtatttac acatccatgt
    48661 gaacctgaag acttatatat acttccacca aaagaatttc tccaagtagc atcatgaatg
    48721 cctatgttcc cattaaatgg aagccaataa cttacaggtg aactgtaacc ttgaccagtt
    48781 aatgaaacat ttctagcctt ataattaaga ggatatatac ctgatggtgt agcaaatcct
    48841 ctagctaaat cacctgttac aacgtctgtt gatacaagca tttctccatt tttaaagaac
    48901 cacatatgtt ggccagaaag actaatctct acatatgtat ttccaatatc atctttatta
    48961 cgagatacag ctttttgtat gtaaataggt tctcttgtaa catttttacc agcttttaca
    49021 acatcaacta atgcttcagc ttctttcttt ttatctatca accaaccata aattccacct
    49081 gaaattgtaa tatctttacc tgtggcagtt ttaaatggtc ttgagtcacc aaaagtactg
    49141 tattttttac ttaaacctct tgtatattct accatagcat cattatcaaa agttatgtca
    49201 taattctttt ctgagtctac ttttaaccat ttattaattt cagaaccatc taaagtttcc
    49261 ttacggtcac taaaatcata tgtaacgact acaccaacat acttatctaa tgtcttaata
    49321 gcttgtttca acttttctga atcctttgtg ttgtttggtg cttcataaac tttactttca
    49381 tccagagaaa tagaagtatt cccagaatta attgagtttt gtactaactt ataaaaatca
    49441 tcttttttca ctttgtttcc aagtacctct ggtactataa cataagcgtt tttagtgcta
    49501 tcatacttag gataagcact cttaggttct acaacatttt tactatcaaa acaagataaa
    49561 gcattaaacc ttttttctag taaatcttta ttatatgaaa gagttatcat attattatac
    49621 tctttagatt taaatattcc accaatccat ccaatagcgc tctgactatc ttttatcttt
    49681 tgaactttat catctggtgt aaattttaaa tcaatatctt ttgcttttat ttgttctttc
    49741 ttattgtttc tttcctctaa gtttaaagta taggaattag tactagatgc taacaaatca
    49801 tttacttgct caactgtttt tcctgaagcc tttacgccat taatagtcgt tccaaagtaa
    49861 aaatgatttc taaaaaagat tgccattcct atataagcta caaacaaaac acctactact
    49921 atagctatct taaattttag atttttttgt ccactttctt gttttacctc ttttccatca
    49981 atcataatta aatccttcct tacattgtaa ttacctatgt accttattgg tttttaaaca
    50041 aaaccatgta tgccttattt tatataccca aattactgta tcattattta tatttttaaa
    50101 tatatagtta tttctataaa accattttat cttttagata aaatgattat tataaatccc
    50161 ccctttcaaa atcccttaaa cacaatctct gctatattaa tatattacca tacttttaat
    50221 aatcgtggga ctatctatct atttttgtaa cactaaacta ctattttaaa aaagaaacta
    50281 attttgtgtt agtttcttta tcttaactta tttttatatt gaattttagc ttaacataac
    50341 gttgttatat agattctatt taattatttt agtttctata tcttttccat ctgcatattc
    50401 tttaagtaat tccttagcat atccaaaata ttcttcaata tcagattctt gactaaaaga
    50461 aaatatacaa attaatattt catttgcatc ttctgttatc attgctcttt cagaatcact
    50521 agttggactc ccaaatttac ccactgaatc agacaatata ggaagatttt ctatatttat
    50581 taattctttc ccaatgccct tatactgttc tccttcatct cctacagtaa aacatactgg
    50641 tgaatgtaat ttgcttacat tatatgtacc tactggatat aatgatttta ttgatattaa
    50701 attgtttatt tctactatat tatttattgt ataaagaccc ttcccttgaa taattcttct
    50761 aaccaatgct tccgaagatg ttctatatct acttggattt tttccaagct ctttataagc
    50821 atttcttgca tctttaattt tatctagttt cgtaatgtct tcaactttta gactttgatt
    50881 tatagtatca caaaactcat ttatttcatt aattaatttt tctgagcttt tatttactgc
    50941 tacttttgct tgaatgcaac ccagcttagc attaggccat ctttccttaa gtttgtcatc
    51001 aatacttaca tctatcatac ttaaacaact cctatacact aaatttttat taaagttttt
    51061 atcatctata cattatagta ctattctttt gttaataaca tgtttattag caaaataaat
    51121 ttatttttaa tataattttt ataaaataca attatttttt gctttatatt agacaataca
    51181 ttgataaaat aaaaaaacac taattattta gcaatgattt attaaaatca agcattcata
    51241 atagtgcttt atataaaaat caaattttgt tttaaagtaa atctaaaaaa ttcttagctg
    51301 gcattggtcg tgcaaaataa aatccttgaa ctaaatcaca tccaaattgt ttcattaact
    51361 cagcttgttc tttactctca acaccttcta taattacaac taaatctagt gattttgcta
    51421 gagacactat tgattttgct atattgcgac ttctctcatc atgttcaatg ccaccaagaa
    51481 aagacttatc tagtttcaat atatcaattg gcatatctct taaacaacta atagaagaat
    51541 atcctgtgcc aaagtcatcc atagaaatag aaaatccatg cttcctcaat tcatttaaaa
    51601 cattctttaa aatatttaac tcattaatag ccacagtttc tgttatctct atttcaatga
    51661 aatctgaact tagattatac atttgtattg ttttattgat aaattttaca atatctggtt
    51721 gatacaactc gactctagat aaatttactg aaactggaac tatcttttta ccttgctttt
    51781 tccattcaga taaatcattg caaacccttt caaatacaaa tctcccaatt tttaaaatca
    51841 acttactttt ctctgctatt ggaataaata ctgcgggact tataaaacca tgttctggat
    51901 gattccatct tattagtgct tcactcccta tcatttctcc agttacaagt gaaaacttag
    51961 gttgataata cacttcaaat tgatttttta ccaatgctat ttttatatca tctaaaatca
    52021 ttgattcttc actaagctta ttcctaacat cttcattata aatagcataa tttatatttt
    52081 tacctttagc aacagaccta gccatatttg ctttatttac agccttttga atatctactt
    52141 caccttcttc aacaaaataa atacccacac acagagagat ttcaaagact atactcaaat
    52201 tactttcaat attacttcgt atattatcaa gttttcttat aattttattt cttccatttt
    52261 tataatcaca gaaaattgca aaataatcac ttgaaagtcg tgcatatacc tcaccttctg
    52321 ttaattcttc ttttaaagct tttgctattg taattaataa attgtcacca aattcatatc
    52381 caaaagtatc attaatcatc ttaaagttat taatatcaaa atatactata atataattta
    52441 aagatggttt atctaaaaga tacttactac actctctata aaatttattt tgatttccta
    52501 tatatgtaac tttatcttca taagccaaac ttaatatttc tttttcatta gattttttta
    52561 tataaattat ataaaaaata attgcaacaa atattaatac aatagataac actgcatata
    52621 cagctctgtt gattatttgt tttgaattac taaatacaac agtttctgga attgaaaaaa
    52681 ttaaattcca gtcttttata taattatttt cactattccc attatccata gtggcatacc
    52741 ctaaatatct tctttcttcc aacatattta atacagttgc acctgtttta ttttcttgca
    52801 attctttttt taatctattt acttcttgaa tatcattatc ctgttctaac aatttataaa
    52861 tattttttga taggctatcc ccttgtggat gaaggataat tgtaccatca ttttttacaa
    52921 cataagatat actattattt tcataaaaag atatattaat aagtttcatc agagtattga
    52981 tattgtaagt acaatataaa actccaacta ctttatcatt atttaataat ggtactgaaa
    53041 tagcaatctt ctttatagac ttatttactt catcaacata tggacttgaa acatacttct
    53101 tacctttaat tgagtttttg aaaaattctc tatctttcac tgaaaactta tcacctttgg
    53161 tattttcagc attaccattt atatcaacta ttgccatagt gtaaaatttt tgattttcca
    53221 tttcatttaa aagatttttc attttttcct cattaggtat atctggattt gaagcataat
    53281 agttagaaaa aatatttaaa acatctaaat ctttttcaat ttgtctttgt gctagttgag
    53341 agccttgtac tgcaacctct tttatatgta tttttgcctc tgacataaga agatgttttg
    53401 tatcttctat atagatatac cccattgtca aaaataatga tgttaaaaaa agaattgtaa
    53461 ttaatgtaaa acttttattt ctccctgtca ttcgactcct cccctaatta gtcaatactt
    53521 catatagata atatatcctt tttataccaa ttcatcattt atattgtata taactattct
    53581 taaatgttac acataaaatc aacataataa ccataaaaac attcttttat tattataata
    53641 acactattta attgaatttt aaattaaaga ataaaaacta tccattttct caaacaaaaa
    53701 aactcttata caataaaact ttgttttatg tataagagtt tttattttat ctattttata
    53761 gcaaacttat cttccataat atgaatttaa atacatatct tttatttcat tcataagtgg
    53821 atatcttgga tttgctcctg tacattggtc atcaaatgct tgaattacca tttcatctat
    53881 tgaatccata aacttagatt cttcaactcc aaactctttt atagatttag gaataccaac
    53941 tttaagtttt aaatcttcaa tagctaccaa taaactttgg aatttctctt catcagaatt
    54001 tccttttaat cctaaaaatg aagctatttc agcatatctc tttaatgctt ctggatattt
    54061 atattgagag aatgctccca ttttctttgg tgcatctgaa gaattaaatt tcataacttc
    54121 tgttatcaaa agagcatttg caacaccatg tggtacatgg tggaaagcac ctaatttatg
    54181 agccatagag tgacaaacac ccaaaaatgc atttgcaaaa gccatacctg ccattgtaga
    54241 agcatttgcc attttttctc tagcttctgg gtcttttgca ccattatcat atgcacgtgg
    54301 aagatattca aatatactct taatagcttg taaagctaat ccatttgtat aatcggttgc
    54361 aagcatagat acatatgctt ctaatgcatg agttaaagca tctataccag aagcagatgt
    54421 taagcttttt ggcatattca tcatcatatc agaatcaact atagccatat ttggcatcag
    54481 ttcataatct gctaatggat attttactcc tgtatcttgg tcagttataa cagcaaatgg
    54541 tgttacttct gaaccagtac ctgctgatgt tggtatagct acaaaattag ctttttcacc
    54601 cattttaggg aatgtatata ccctttttct tatatccata aatctcatag ctaggtcttg
    54661 gaaatctaca tctggatgct catacaatgt ccacattatc tttcctgcat ccattgcact
    54721 tcctccacct atagatatta ttacatctgg attaaattct ctcatagctt tagcgcctat
    54781 cttagcacat tccaaagttg ggtctggttc tacttcaaag aatgtagtgt gcttaatatt
    54841 catttcatct aataaatcag taactgcttt agtatatcca ttattgtaaa ggaatgtatc
    54901 tgttacaata aaagctctct tcttattcat tacatcttta agttctttta atgctactcc
    54961 taaacaacct tttttgaaat aaactttttc tggtgctcta aaccaaagca tattttctct
    55021 cctctcagct actgttttaa tattaagtaa atgcttaact cctacatttt cagatactga
    55081 atttccaccc caagacccac atcctaaagt aagagaagga gctagtttaa aattatataa
    55141 gtcccctata ccaccttgag aagaaggagt attaattaaa attctacaag ttttcatggc
    55201 tgaagtaaac ttatcaagtt tttctctttg atttacatca tcaatatata atgaagatgt
    55261 atgcccaaat ccaccatctt ctactaattt ttctgctttt ctaactgcat catcaaaatc
    55321 acttgccttg tacattgcaa gtactggtga aagtttctca tgagcaaaag cttcttctat
    55381 ttcaactgat tctacttcac ctattaaaac cttagtatct actgggactt caaagcctgc
    55441 aagttttgct atagtacaag ctgtttgccc aactatttta gaatttaatc ctccatttac
    55501 taaaattata tttcttattt tttctgtttc atctttatct aataaataag caccacgctc
    55561 tttaaattct tttttgactt cattatatat attttctaat actataaccg attgttcaga
    55621 agcacatatc ataccattgt caaaagtctt agatacaaga attgagttta cagcagtttt
    55681 tatatctgca ctatcatcaa ttattgctgg agtattacct gcaccaactc caatagctgg
    55741 ttttccagat gaatatgctg atttaaccat tccaggacct cctgtagcaa gggtcaaatc
    55801 cacttctttc ataacagttg tagttaattc taaagatggc tcatctatcc atcctattat
    55861 accttcagga gctccagcta aaacagcagc ctcaagaaca acttttgcag cctctatagt
    55921 agaatttttt gctcttggat gtggagagaa aattattcca tttctagttt ttaaagctaa
    55981 aagtgcttta aagatagcag tagaagttgg attagttgta ggtacaactg ctgcaataac
    56041 acctattggc tcagcaattt tagtcatacc aaatgcttcg tctttctcta taacaccaca
    56101 agttttagtt tctttatatg cattatatat atactctgag gcataatgat tctttataac
    56161 cttatcttca gcaattccca taccagtttc tttttgtgcc attatagcta gaggaactct
    56221 ttgcttatta gctgccaaag atgctgctaa aaaaatttta tctacctgct cttgactata
    56281 tgtagcaaat attttttgag cttctctgat ttttgctagt tttttaacta agctttcaac
    56341 gttatttaca gtatctaaac caactgacat aacttcattt tgttttttat tttctattat
    56401 tacttgttct ttatgagcca ttttaacaac ctcctgagat agttttttat ttattatgtt
    56461 aatttattaa cattatagca tttacttttt ctattttcaa gttatttttt taacatttta
    56521 ttttttatct aatataacat catttctact aataatatct agctttacaa atacttaatt
    56581 taactagata tcactttatt ttttacacaa atcactaatg aaatcatcta aaaacttaag
    56641 gataacgtta tttttttaac gtttatattt ttgtattttt tacaaataaa actttataaa
    56701 aagaattttt aatatgatac tctatcaagt ttaaaaataa aaatacttga aaatgaaaat
    56761 aaaaacttaa ttaacaagtt aaagtaaata tagttaa
//
LOCUS       DAMJYC010000016        56324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJYC010000016 DAMJYC010000000
VERSION     DAMJYC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.017"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC472_001035"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC472_001035"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="PC472_001036"
     tRNA            179..253
                     /locus_tag="PC472_001036"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="PC472_001037"
     tRNA            259..344
                     /locus_tag="PC472_001037"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="PC472_001038"
     tRNA            360..435
                     /locus_tag="PC472_001038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="PC472_001039"
     tRNA            443..517
                     /locus_tag="PC472_001039"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="PC472_001040"
     tRNA            532..605
                     /locus_tag="PC472_001040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="PC472_001041"
     tRNA            611..686
                     /locus_tag="PC472_001041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="PC472_001042"
     tRNA            692..768
                     /locus_tag="PC472_001042"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="PC472_001043"
     tRNA            779..853
                     /locus_tag="PC472_001043"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="PC472_001044"
     tRNA            863..936
                     /locus_tag="PC472_001044"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
     gene            1920..2759
                     /locus_tag="PC472_001045"
     CDS             1920..2759
                     /locus_tag="PC472_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2726381.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2756..3937
                     /locus_tag="PC472_001046"
     CDS             2756..3937
                     /locus_tag="PC472_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726382.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4241..5143
                     /gene="ptb"
                     /locus_tag="PC472_001047"
     CDS             4241..5143
                     /gene="ptb"
                     /locus_tag="PC472_001047"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2726383.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5165..6244
                     /gene="buk"
                     /locus_tag="PC472_001048"
     CDS             5165..6244
                     /gene="buk"
                     /locus_tag="PC472_001048"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2726384.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6234..6911
                     /locus_tag="PC472_001049"
     CDS             6234..6911
                     /locus_tag="PC472_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726385.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6954..7169
                     /locus_tag="PC472_001050"
     CDS             6954..7169
                     /locus_tag="PC472_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2726386.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7198..8277
                     /locus_tag="PC472_001051"
     CDS             7198..8277
                     /locus_tag="PC472_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2726387.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8277..9029
                     /locus_tag="PC472_001052"
     CDS             8277..9029
                     /locus_tag="PC472_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2726388.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9030..9587
                     /locus_tag="PC472_001053"
     CDS             9030..9587
                     /locus_tag="PC472_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2726389.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9757..11103
                     /locus_tag="PC472_001054"
     CDS             9757..11103
                     /locus_tag="PC472_001054"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2726390.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSLPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATDLANLIQEKLS"
     gene            11370..13202
                     /gene="glmS"
                     /locus_tag="PC472_001055"
     CDS             11370..13202
                     /gene="glmS"
                     /locus_tag="PC472_001055"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2726391.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13654..14949
                     /locus_tag="PC472_001056"
     CDS             13654..14949
                     /locus_tag="PC472_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2726392.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYLDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15433..16170
                     /locus_tag="PC472_001057"
     CDS             15433..16170
                     /locus_tag="PC472_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2726393.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            16191..17444
                     /gene="murA"
                     /locus_tag="PC472_001058"
     CDS             16191..17444
                     /gene="murA"
                     /locus_tag="PC472_001058"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2726394.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17531..18595
                     /gene="spoIID"
                     /locus_tag="PC472_001059"
     CDS             17531..18595
                     /gene="spoIID"
                     /locus_tag="PC472_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2726395.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18661..19329
                     /locus_tag="PC472_001060"
     CDS             18661..19329
                     /locus_tag="PC472_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2726396.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19427..19687
                     /gene="spoIIID"
                     /locus_tag="PC472_001061"
     CDS             19427..19687
                     /gene="spoIIID"
                     /locus_tag="PC472_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2726397.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19884..20903
                     /locus_tag="PC472_001062"
     CDS             19884..20903
                     /locus_tag="PC472_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2726398.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20919..21347
                     /gene="fabZ"
                     /locus_tag="PC472_001063"
     CDS             20919..21347
                     /gene="fabZ"
                     /locus_tag="PC472_001063"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2726399.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21458..21967)
                     /gene="yyaC"
                     /locus_tag="PC472_001064"
     CDS             complement(21458..21967)
                     /gene="yyaC"
                     /locus_tag="PC472_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2726400.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22156..22259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22396..23589
                     /locus_tag="PC472_001065"
     CDS             22396..23589
                     /locus_tag="PC472_001065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2726401.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23905..25002
                     /locus_tag="PC472_001066"
     CDS             23905..25002
                     /locus_tag="PC472_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726402.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPVIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25266..27485
                     /locus_tag="PC472_001067"
     CDS             25266..27485
                     /locus_tag="PC472_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2726403.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEILKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27488..28270
                     /locus_tag="PC472_001068"
     CDS             27488..28270
                     /locus_tag="PC472_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2726404.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28602..30515
                     /locus_tag="PC472_001069"
     CDS             28602..30515
                     /locus_tag="PC472_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2726405.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIQGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30656..30964
                     /locus_tag="PC472_001070"
     CDS             30656..30964
                     /locus_tag="PC472_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2726406.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30988..31314
                     /locus_tag="PC472_001071"
     CDS             30988..31314
                     /locus_tag="PC472_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726407.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31353..32687
                     /gene="celB"
                     /locus_tag="PC472_001072"
     CDS             31353..32687
                     /gene="celB"
                     /locus_tag="PC472_001072"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2726408.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32698..33216
                     /locus_tag="PC472_001073"
     CDS             32698..33216
                     /locus_tag="PC472_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2726409.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLTDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33312..34271
                     /locus_tag="PC472_001074"
     CDS             33312..34271
                     /locus_tag="PC472_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2726410.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCVATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34320..35372
                     /locus_tag="PC472_001075"
     CDS             34320..35372
                     /locus_tag="PC472_001075"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2726411.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35375..36049
                     /locus_tag="PC472_001076"
     CDS             35375..36049
                     /locus_tag="PC472_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2726412.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVHNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36602..37153
                     /gene="raiA"
                     /locus_tag="PC472_001077"
     CDS             36602..37153
                     /gene="raiA"
                     /locus_tag="PC472_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2726413.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37285..39960
                     /gene="secA"
                     /locus_tag="PC472_001078"
     CDS             37285..39960
                     /gene="secA"
                     /locus_tag="PC472_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2726414.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39975..41082
                     /gene="prfB"
                     /locus_tag="PC472_001079"
     CDS             join(39975..40049,40051..41082)
                     /gene="prfB"
                     /locus_tag="PC472_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2726415.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41226..43367
                     /locus_tag="PC472_001080"
     CDS             41226..43367
                     /locus_tag="PC472_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2726416.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43515..44681
                     /locus_tag="PC472_001081"
     CDS             43515..44681
                     /locus_tag="PC472_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726417.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEEFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44741..44858
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44983..45588
                     /locus_tag="PC472_001082"
     CDS             44983..45588
                     /locus_tag="PC472_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2726418.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45917..46957
                     /locus_tag="PC472_001083"
     CDS             45917..46957
                     /locus_tag="PC472_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2726419.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            47096..47548
                     /gene="tsaE"
                     /locus_tag="PC472_001084"
     CDS             47096..47548
                     /gene="tsaE"
                     /locus_tag="PC472_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2726420.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            47548..48264
                     /gene="tsaB"
                     /locus_tag="PC472_001085"
     CDS             47548..48264
                     /gene="tsaB"
                     /locus_tag="PC472_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2726421.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48254..48730
                     /gene="rimI"
                     /locus_tag="PC472_001086"
     CDS             48254..48730
                     /gene="rimI"
                     /locus_tag="PC472_001086"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2726422.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48736..49752
                     /gene="tsaD"
                     /locus_tag="PC472_001087"
     CDS             48736..49752
                     /gene="tsaD"
                     /locus_tag="PC472_001087"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2726423.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            50049..52757
                     /gene="hpdB"
                     /locus_tag="PC472_001088"
     CDS             50049..52757
                     /gene="hpdB"
                     /locus_tag="PC472_001088"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2726424.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52761..53018
                     /gene="hpdC"
                     /locus_tag="PC472_001089"
     CDS             52761..53018
                     /gene="hpdC"
                     /locus_tag="PC472_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2726425.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            53011..53961
                     /gene="hpdA"
                     /locus_tag="PC472_001090"
     CDS             53011..53961
                     /gene="hpdA"
                     /locus_tag="PC472_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2726426.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            54277..55215
                     /locus_tag="PC472_001091"
     CDS             54277..55215
                     /locus_tag="PC472_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2726427.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(55337..56315)
                     /locus_tag="PC472_001092"
                     /pseudo
     CDS             complement(55337..56315)
                     /locus_tag="PC472_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT        22569 a         6084 c        10669 g        17002 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaagtta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa aattagagac tatagtgaat
     1321 ttacaattta atgtaatatt tataagttat actaaataaa ttattatact gtgaaatgga
     1381 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
     1441 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
     1501 gaaataagtt attactttaa tataaaaatg aatctaatgg aataatattg atgataagta
     1561 atatttagat tttgtatttg tttatagtta ataagtggat tatagaattt ataataatta
     1621 atatatatta aacaaagata tattaagata ttttagaaca caaaactgca ttaaggcgcg
     1681 agtactattt tgtattcgcg ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa
     1741 taagaaatct gtatttatta agttgatatt tttaatgtgt ttagagatta tttaacaatc
     1801 tccaaatata aaactagaat tatttacata aaagttaaat agtttgtata gagttagatt
     1861 tatatagtat aatacttagt gtaaataatt aagaagttat caaacaggag gataaaactg
     1921 tgttagatat aaattctttt ttaaatggat tttttaatat aatattaaga atgagtatat
     1981 atgatttaat tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa
     2041 aagatacaag agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac
     2101 ttagtaacac ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg
     2161 gagttatagc tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag
     2221 gaagagcaaa gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata
     2281 gaaatataga agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc
     2341 ttatcataat ggagagagaa actagaataa gtgatataat aaatacagga actattattg
     2401 atgctgaaat ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg
     2461 atggagctgt tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga
     2521 cagaaagtaa ggatttaagt aaagatttgg gtactagaca tagagcagga ataggggtaa
     2581 gtgaggtgtc tgactgtata actttgattg tatcagagga aacaggtggc gtatcaattg
     2641 ctaaagcagg taagttatat agagatattt ctagagaaag aatgatgaat atattacgta
     2701 gtaatttaaa aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat
     2761 aaatagacta aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt
     2821 atggatgtat gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc
     2881 tgtatctata agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga
     2941 aaaattaaca gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaaaaa
     3001 agaagatata catgtatatg gaaccattga agacccaata caaggacaga aggaagtata
     3061 tttaaaagca aatattcctc aaggtgttac tctagaattt aagcatgatg ttttagtagt
     3121 aaatcttgaa aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc
     3181 aaaaagaaat ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag
     3241 aactctagtt aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga
     3301 tgatttttct aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg
     3361 agtaacatta agttcatcct ttgtgactgc aaagatagaa ctattgaagg aaaaaattgt
     3421 cccaataaga gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt
     3481 aagtaaggaa caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat
     3541 taagacaaaa cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt
     3601 agaaattcca gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac
     3661 aaaacaattg agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc
     3721 aacaaatatg gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc
     3781 aacggaggat ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca
     3841 aggccctgag gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa
     3901 aataaatata gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt
     3961 gttacaagat gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg
     4021 catcttgttt attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa
     4081 aaagcgctag atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa
     4141 atatttcaat tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa
     4201 aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt
     4261 aattaagttt gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa
     4321 agaagtttta atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt
     4381 aggagatata gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta
     4441 tgaactgata gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc
     4501 acaaggaaaa gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc
     4561 agttttaaat aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt
     4621 tgatgtagag ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc
     4681 tgatacaaat actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga
     4741 tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa
     4801 agatacagtt gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg
     4861 tatggttggt ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa
     4921 aggtataaat catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg
     4981 tggtaacata ttatataaag ctttggtatt cttctcaaaa tcaaaaaatg caggagttat
     5041 agttggggct aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact
     5101 aaactcaata gctttaggtg ttttaatggc agcaaaggca taattttaag ggggaaatag
     5161 gcaaatgagc aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat
     5221 agctgtattt gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga
     5281 aataggaaaa tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga
     5341 agctctaaaa gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg
     5401 acttcttaaa cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt
     5461 aaaagtggga gttttaggag aacacgcttc aaacctaggt ggaataatag caaaacaaat
     5521 aggtgaagaa gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga
     5581 agatgttgct agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt
     5641 aaatcaaaag gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat
     5701 aaatcttata gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa
     5761 aatagtagat gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg
     5821 tggactacca gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga
     5881 aattaaaaag aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc
     5941 tagagaagtt gaagaaagaa ttgaagctgg tgatgaaaaa gctaaattag tatatgaagc
     6001 tatggcatat caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt
     6061 aaaagcaata ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc
     6121 agatagagtt aaatttatag cagatgtaaa agtttatcca ggtgaagatg aaatgattgc
     6181 attagctcaa ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa
     6241 ctaatgataa aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg
     6301 cgatgaacta tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt
     6361 tagatgtggt aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag
     6421 gtatacaacc aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag
     6481 cagaggtaat gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag
     6541 ataatgtagg tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg
     6601 atatgctttt tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc
     6661 aatatataaa agaaatagaa aaatcttcta aattaaaggt aacaggtcta attaataata
     6721 ctcatcttat taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag
     6781 aagtttctga aaaatgtaat ataccgataa gatatgttgc atgtttagaa aatcttgttg
     6841 aacagttacc aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag
     6901 actggatgta gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta
     6961 aaggaaaagt atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat
     7021 gtccagtaaa aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag
     7081 caactgtttt tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag
     7141 atgtagttat aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg
     7201 gctaagatac ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc
     7261 aaatatttct tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga
     7321 gagttaccta aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac
     7381 atggtttatg gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga
     7441 gttgctttaa agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta
     7501 cttaatgtaa tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac
     7561 tttatgtcta caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct
     7621 acagttcaag aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga
     7681 tcaccagtta tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt
     7741 agagctcctg agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa
     7801 ggaaaaagaa aacctaatgt aataaactct ctatacttag agccagaagt actagaagac
     7861 cattgttggc atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa
     7921 atgtacaaaa cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta
     7981 gtaaaaagcg caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct
     8041 aaagtattat ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata
     8101 ttgactgttg aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt
     8161 aaattaccag tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata
     8221 gttgaaaaag ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg
     8281 ctgttgtatt taagaaaact gaaggattac aagatactca aactcactac tgtccaggat
     8341 gtacacatgg tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag
     8401 gtgatgctgt aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact
     8461 gtgacactca agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag
     8521 ttcatcctga aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta
     8581 ctgctgaaat agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca
     8641 atacaactta tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag
     8701 ctactactgc tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg
     8761 aaatgttagc tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag
     8821 ctcatgttag acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat
     8881 taggatttgg aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa
     8941 atgatgcttt acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca
     9001 aaaatgttga agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct
     9061 tcggaggcca aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag
     9121 gaaaagaagt ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact
     9181 gtgcagtaac tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg
     9241 cagctatatt attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg
     9301 gaaaaataat agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg
     9361 tttattatat accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaaca
     9421 tgataatgtt aggcgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag
     9481 aagcattcaa aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag
     9541 ctttacaaaa aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat
     9601 gtcaaaaaaa agacaataga attatttcta ttgtcttttt tgctgataag tattaaaata
     9661 tttttatgac tgaaattaag cctataatga tattttatga actataaaaa tataatacaa
     9721 attacaagct tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat
     9781 ggagttagag gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga
     9841 gctggtggat ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat
     9901 actagaatat caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga
     9961 tgtgatgtta ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa
    10021 tatggtgctg attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga
    10081 atcaaattct ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa
    10141 gaatatatag acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga
    10201 aaattacatg aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc
    10261 actgacttta agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg
    10321 gcaccaatag tgtttgatga actaggagca agtgtaattt caataaatag tttaccagat
    10381 ggaaataata taaactataa atgtggctct acacatccag aacaacttca aagggctgtt
    10441 ttggaacata atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca
    10501 gtaaatgaaa agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat
    10561 ttaaagaaaa ataataaatt ggcacaagat actttggtag ttacagttat gagtaatata
    10621 gggttgacaa tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac
    10681 agatatgttt tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga
    10741 catatgatat ttttagatta caacactact ggagatggag tgcttagttc tctaatactt
    10801 gcaaatataa ttttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa
    10861 tacccacaag tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa
    10921 tatccagaaa taaaaactga gatagaaaga atagaatcta ttttagacgg aaatggtaga
    10981 gttttgataa gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag
    11041 gaaggtcaaa taaaagaatt agcaacagac ttagctaatc ttatacaaga aaaattatcg
    11101 taatttaaag ggtattctca gatacaaata ctgaggacac ctttctaagc gccagaactt
    11161 agttatagac ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg
    11221 aattgtcggc ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga
    11281 gcaatcctat ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata
    11341 taaataatat ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta
    11401 gaaaggcagc agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact
    11461 cagcaggagt agcagttaat agttctaatg aaaaagaact taatattaga aagtttaaag
    11521 gtagactttc tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta
    11581 tagggcatac gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt
    11641 ttaatcaagc caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa
    11701 taaaagaaga attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa
    11761 tagcacattt agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta
    11821 tatcaaaatt aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat
    11881 tagttgcagt aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta
    11941 tagcatcaga tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg
    12001 gagaagtagt tcatctaaaa gacgaaaatg taaccgttta tgactctaat agaaatcttg
    12061 ttgaaaaaga agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg
    12121 actactttat gtcaaaagaa attcatgagc aaccaacagg agtaagagaa actttagaaa
    12181 gaagacttga tgataatgga aatataattc tagacagtat aaatatttct aaagaagact
    12241 tagaaaaaat aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac
    12301 ttggtaagta tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag
    12361 aatttagata tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagtcaat
    12421 ctggtgagac agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa
    12481 tactttcaat aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct
    12541 atacttgggc aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa
    12601 cttctttata catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat
    12661 tttatgatag tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatactag
    12721 acaatgaaga atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct
    12781 atttaggaag aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga
    12841 tttcatatat acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt
    12901 taatagaaaa aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa
    12961 tggtttctaa tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa
    13021 gccataataa ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg
    13081 atattttatc accaatatta gcagttgtac caatgcaatt acttgcttat catgtctctg
    13141 tactaagagg atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat
    13201 aaaaacagat taaaaattaa atagataata tttattgaaa agaactcatc ctgttatttg
    13261 tattttatag gatgagtttt ttagataaat attaaatttg ttttgttttt gctataataa
    13321 attttataaa aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca
    13381 ggtataggga acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata
    13441 gtcaatatca gataaaaaaa ataatagctt atgagaaaaa ttaaaataca aggtattagt
    13501 aaccttgtga taagataaag tgagtagttt aaaataagaa tataaggaac tatttataaa
    13561 agaatataat gaagctaata gagtataaga aaattacttt attttagtaa aataatttca
    13621 aataaaaatt tttttaaatt gggagtataa attatgacaa atgattttaa aaaagaaaag
    13681 aaagaagatt attttgttaa tcttaaggca atatctactg aagttttaca agaaaaagtg
    13741 caagataacg cttgggttat agttgatact cgcttaaatg atgcttataa tggctggaag
    13801 ttagatggag taaaaagagg tggacatata aaaggagcag tagatttttc tgctaattgg
    13861 ttaagtgtat acttagatag aaaagatgaa gtgttagaac aagcattgaa aactaaaaga
    13921 attgatttag ataaaaatat agtattgtat gatgcaaatg gaaaagatgc tttggttgta
    13981 gcagattatt tatctaaaaa aggatataaa tatctatata aatatgatat taaacaatgg
    14041 gcagacgacg aaaatcttcc aatggaaaga tataaaaatt atcaaatgat agttcctgct
    14101 tttataataa aagatatatt agatggaaaa attcctgaga catttgaaga ttcaaagaat
    14161 ataaaaatga ttgaagcgag ttggggagaa gaatcgtata caaaagggca tatacctaca
    14221 agtgttcatg taaatactga tattatagaa cctcctccaa catggatgct tgataatgat
    14281 gataacttaa ctaagtttgc tttagactat ggattaacaa aggatgatac tgtaatagtt
    14341 tcaagtagta cacctatggc atcttataga ctagctgtta tattgagata tataggagta
    14401 aaagatgtaa gagttttgaa tggtggaaca aactcatggt tatcagcagg atatgaatta
    14461 gaatttatta gtaatccaaa acattcatgt actaattttg gagcagatat accagttaac
    14521 tcacaattag ttgttactac atctgaatta agacaaaaac taaaagaaaa aaataagttt
    14581 atactagtag ataaccgtac ttgggatgaa catataggaa aagtatctgg atatacatat
    14641 tatgataaaa aagggcgtat accaggagct ttatatggac attcaggtag tgatagtgtt
    14701 tcactagaag aatatcgtaa tattgataat acaatgagaa ataaatatga gattttagaa
    14761 atgtgggaca aggaaaatat agatgtgaat aaacagctta tatttatgtg tggaagtgga
    14821 tggcgtgctg ctgaagtatt aacatatgca aatgtcatag gagtggaaaa tacctccctt
    14881 tatagtgatg gatggatggg ctggagtctt gataattcaa atttaattga agtgggagaa
    14941 cacaaataaa gtttttgaaa gtacaataga gttgtttaaa acaaaggtgt ttttgattta
    15001 ttaggaagag tgtgtaaatt gtgtattggg aagtggactt aggtatgttc atttggagct
    15061 atggaaactg ataaaaaagt attatctgtt tattttgctg gtaaaatatg aatagattat
    15121 agattataga ttatagatta tagattatag attatagatt atagattata gattatagat
    15181 ttaagtatag actaagtaat ttatattaca gaagagaata tataagaatt aatgcaagat
    15241 gtatttgata catataagaa attggcaagt ccaggagaag gatagagaaa attgagattg
    15301 aagagtttaa aagagattat tagatgaaaa atacaaaaat gtataaaaat acatattgag
    15361 aaagtatatc tctcttaaat ttgttaataa ttctattata cagaaaaaca tatttaaggg
    15421 ggatttttta ctatgaaaaa agtaaaatta gctataagct tttttatatt aatgattata
    15481 ggaatgacaa tatcttatgc agatacaagt ataaatacta gatacaatag attaagtaaa
    15541 gctcttgaaa tatcagaagc aaattttaaa ttttataatg ttaaaattaa ttctgttata
    15601 aatcataatt tatcaaataa agaatttcat aatattttta tagaaataat taacaatcta
    15661 aagttggata aaaaaggaat aaattggaat caaaaagggt ctggcgataa aatacaatta
    15721 tgtgctacta taaaaggtag cgataaagaa atttctataa aaggaataaa taaaaataac
    15781 aaagaaagtt acatagtagt tgacatattg gataacaaag tatataaaca taaagaggtt
    15841 atttatagta acgtagaaaa tgtgttaaat aaatattcat ctcaagtaga tgtgtctgct
    15901 tgtattgttg gagaatacac aaaaaggtta caaaacaaca aatatgatga tattttacaa
    15961 aaaatattat ataatatgag tgctaagcaa atagatgaag ttaaagacgt aaacttttta
    16021 tctataacag catatagtaa agtattaaat gattatgaat tagaatattt aggggaaaaa
    16081 attaatttaa atataggaat taaatataat gaggatgacg gaaaaacact catatatatt
    16141 gccacaccaa ttataaaatt agattattaa ttatgaggag atcaaaaaat atggctaaga
    16201 taatagtaaa aaagagtaat ccacttaaag gtagtgttaa aatagatggt gcaaaaaatg
    16261 cagttttacc aataatagca gcgactttat tagcaaacgg aaaatcaaca ttaaatggag
    16321 taccaaattt aagagatgtt catgttatat cagatttatt aagacatgta ggagcagaag
    16381 ttgaatataa agaaaataca cttactgttg atgcaagcaa tataaaaact tgtgaagctc
    16441 catatgagct tgtaagaaag atgagagctt cttttttggt tatgggacca ttgcttgcta
    16501 gatttaatag tacgaaaatt tctatgccag gaggatgtgc tataggaaca agacctatag
    16561 atttacattt aaaaggattt aaagccttag gtgcaaagat agaaatggac catggttttg
    16621 ttgaagcagc aacagaaaag ttagtaggaa ataaattata tttagatttc ccatctgttg
    16681 gagcaactga aaacataatg atggcagctt ctttagcaga agggactaca ataatagaga
    16741 atgcagcaga agagccagaa atagtagatt tagcaaactt cttgaacgaa atgggagcag
    16801 atgttaaagg tgcaggtact aatactataa aaataaaagg tgttaaagag ttaaaaggag
    16861 cagaacataa tgtaattcca gacagaatag aagcagcaac ttatatggtt gcagcagcta
    16921 tgacaaaagg tgatataact gttgaaaatg ttttaatgga gcatttaaaa ccagttgtag
    16981 ctaaattaag agaagctggt tgtgaaataa cagaaatgga caattctgta agagttgtag
    17041 gtccaaaagt attaaaacca atagatataa aaactttacc acatccagga tttcctacag
    17101 atgttcaagc acaatttatg gctatgctta ctgtagccaa tggaactggt gtagttatag
    17161 aaactgtttt tgaaaacaga tttatgcatg ttgctgaatt taatagaatg ggtgctaata
    17221 taaaaataga tggaagaagt gcggttgtta atggtgtaga tgagctacac ggtgcagcag
    17281 taaacgctac tgatttaaga gcaggggcag ctcttatttt atgcggtctt atagcagaag
    17341 gagaaactca aataggagaa atataccata tccaaagagg ttatgttgat atagataaga
    17401 agataacagc actaggtggt caaatcgaaa tagtagaaga ttaataattg gtttatagaa
    17461 gaaagttata aattaatagg gttgttcata tacttaaata caaggtttta aaagctaagg
    17521 aggagagtat atgaagaacc cattggttgt tttgttagga tttgtgactt gctctgtgtt
    17581 agttccatca cttataactt tagtttctta taaaaatgta gaattaactg aaaaaccaga
    17641 gagcccagta tctataaaca aaactattaa aaaaagtgat attgaagaca aaggtaataa
    17701 agaagaaaaa aatacagtga actatgaaac tgtaaataaa aaagcaccaa ttatcaatgt
    17761 atataatcat ataacaggga aaaccgaaaa gatggacatg gagaactatt tatgtggagt
    17821 actagctggt gagatgtctt cagagtttga tatagaagct ttaaaagctc aatctgtagc
    17881 agctagaaca tatgtagtat ataaacagga acatggtaaa tctagtaaac ataaaaatgc
    17941 agtagtatgt actgattata aacattgtca agaatataag agttatgata ctcttaaaaa
    18001 attaaatggt gaagaatgga ttaagaataa atattccaaa atccaagaag cagttagagg
    18061 aactaaaggt caaataataa cttataatga taaggcgata cttcctcttt atttttctac
    18121 atcttcagga aaaacagaaa atagtgaaga agtattttct gcaaaatatc cttatttaaa
    18181 gtctgtggaa agtccttatg ataagtattc ccctaaattt gcgtcaacac ttaaaataag
    18241 taacacagat tttgttaaaa gtcttagaag ggcatatagc actattgtga tagatgtgaa
    18301 taatttaagt aaacaagttt caataactaa aagaagtgat gccggaactg tagaaaaaat
    18361 aaaattagga aataaagaat tgactggaaa agacattaga actgttttta aattgaactc
    18421 tgcaaacttt gacataaaat ttggagaagg atacatagat ttcgtggtaa aaggatatgg
    18481 tcatggtgtt ggtatgagcc agtggggagc agaaggaatg gcagaagagg gctataaata
    18541 ttatgatata ttatcacatt actacacaga tacaaaaata aaagatatat actaataatt
    18601 gtataaaatg ccaaaaacac gtcaataatc caaatgtaga aatatatttg gaggtgcaaa
    18661 atgaagaaaa agctgttaga aaaagatggt ttttatttat ctttgtttgt atgtgtttgt
    18721 ttattagcag taggtggagt ttggtttaca aataataatg tagataaatt ggcttctaat
    18781 aaaggaatta tggaaaatgc taataaagat agtgaagagg aaatacattt aattgaaaaa
    18841 gacaagaaag atgctatccc tactgcaacg gattcaaaac aaaacttaga aaaagctaag
    18901 tcaaaagagg aaaataaatc gagtacaaca aaattaaatt acattggaga taaagtaata
    18961 agaggatatt cagaaaaaga gcctagttat tctaaaacac ttgatgtttg ggaaactcat
    19021 aaaggtgtag atattagttg tactaaaggc aaagaagtaa aatctctatt aaacggaata
    19081 gtagtagatg tatttgatga tgaagaatat ggacaatcag tcaaaataaa aagtgacaat
    19141 aacattgtag tagtatattc aaatctagat aaaaatgtta gtgttaagaa agaacagaag
    19201 gtaacagaag gacaatctct aggtactgta ggtagtactt ctcaaataga aagtgaagaa
    19261 ggtatacatg tgcatttaga ggcgtatagt ggagaaaaat ctatagaccc aatgagtcta
    19321 attaagtaat agtataacaa gtttaatcat atttggttaa gcttgttata ttttttggtg
    19381 tttttacata tatatcatta aagctattat gtgagggggg taatctttga gatctcatat
    19441 agaggaaagg gccatagtgg tagcaaaata tatacttgaa aaaaacacta cagtaagaca
    19501 aaccgccaag acatttggag taagtaaaag tacaattcat aaggatgtta cagaaagatt
    19561 gaaggaaata aatccatccc ttgccaaaga agttaaaaat gtgctggaca aaaataaatc
    19621 agagagacat attagagggg gattagcaac gaaattaaaa tatgagaagg aacataaaaa
    19681 aatgtaggtt aaaaacctac tttttttatt gaattaaatt ataaaaagaa atatattaag
    19741 taattataaa tagcaaaata ataatactct caaaagattt attttctctt gattaaagcg
    19801 agatttttgt atatgcttta ttataggagt attaaaatag gcttataata taatctaata
    19861 ttataaataa aggagttgaa aaaatggctg gagctgatat aggcatagat ttaggtactg
    19921 caaatgtgct tgtttatgtt agtggtaagg gaatcgtttt agaagaacca tctgttgtgg
    19981 cgatagacaa aagaacagat tctgttttag ctgtgggaga agaagctaaa aagatgatag
    20041 ggagaacacc aggtaatata gtcgctataa gacctttaag agatggtgta atttcagatt
    20101 atgatgtaac tgagaagatg ttaaaatctt ttatagataa gatagttgat aaaaagggat
    20161 ttggaagatt ttttatgcca agaattatgg tatgtgtacc aacaggtgtc acagaagtgg
    20221 aaaaaagagc agtagaagat gctacgagac aagctggagc tagagaagta tatatcatag
    20281 aagaaccaat agcagctgcc ataggagctg gtgtagacat atcacagcct aatggtaata
    20341 tgattataga tattggtgga ggtactgtag atatagctgt aatatctcta ggaggagctg
    20401 ttgtaagtga atctataaag gttggtggag atagatttga tgatgctata gttaaatata
    20461 tgaaaaaaca acacaatctt cttataggag aaagaacagc agagaaaatc aagtttgaaa
    20521 taggttctgc atttaagaga gaagaagaaa aatatatgaa gataacagga agaaatttaa
    20581 ttacaggact tcctagttct ataactataa attcaacaga aatgttggaa gcacttagag
    20641 aatgtgttga gcaaatagta gttgctactc atgctgtttt ggaaaaaacg cctcctgagt
    20701 tagcagcaga tataggagat gtcggtataa taatgactgg aggaggttca ttattatatg
    20761 gattagataa aataatagaa cagaggacag gaataactgt tacaatagca gatgaaccat
    20821 tatcttgtgt tgcaaaaggt acaggaatag ctttaggttc aatagactta cttgaaactg
    20881 gtgggtcttt caaaagaaaa taattaggag agtttagaat gttaaatata gatgaaataa
    20941 aaaaattaat accacataga tatccatttt tattagtaga caagattaca gagctagaag
    21001 ttgggaaaag agctgttggt ataaagaatg taacagttaa tgagccattt ttccaaggtc
    21061 actttccaga atatcctttg atgccaggag tactaatagt agaagctcta gcacaagtat
    21121 gtggagtggc aatgatgtct gtagaggaaa acaaaggaaa actaggtgta tttgcaggta
    21181 tagataaagt tagaatcaag agagaagtaa gaccaggaga tacattaact atggaagttg
    21241 aaatgacaac tcttagaaaa aatatagcta aggcagatgc taaggcatat gtaggtgaag
    21301 aacttgtctg taaaggtgaa ttaatgtttg ctttagtaga aaaataaaaa cttgaaatat
    21361 aaaagtaaaa aggtagataa tttggagtat ttaatcagat actctaaaat atctaccttt
    21421 ttatataaag aattttatca tttagtttat atttagatta ggtggatact aataaagcca
    21481 aggctattgt ttcagctaaa tctaatataa agctaaggcg tatattatta aatgaaagtt
    21541 tattattttc atcaatttta tctacaattc ctactataga ataatttcct atttgaggta
    21601 gttttttacc aacacctttt ccgggcagga taggtccttc tctaatctgt atattaccta
    21661 tgttactctg cgaccccaag caagcatcta tagccaagaa gttaccttgt atatgtgtat
    21721 tttttatagt atctaaagac tcatatatat ttaaggcgtg aatagggtta tctagtgtac
    21781 cgtaaacggg gtatttaaag ttactgttct taagtattgt tccaactaaa gggcctaaag
    21841 tatcacctat agccctgtct gttcctatgc aaacaacaat cgtattttca ttaattatag
    21901 tttttagaac tgaacttagc atctgtataa catcatcatc gctgtagttt actttagcta
    21961 aatacaatag gaacaccccc ttcaaaaaaa tatatgaata aaaagcatta aaaagaccta
    22021 actttaaatt tcagatagat agaaaatttt atttgtctta caaaaaaagg aatagaaaaa
    22081 atatgactaa aaatattgac aaaattttaa aaaaatagta agctagtaaa gtaaaacaaa
    22141 acttaaatct tgattacctt atcaagagag gctgagggat aggccctgtg aagcccagca
    22201 accacccaaa tgggaaaagg tgctaattcc tacaagacaa aagtcttgaa agatgaggtc
    22261 agttaattag tatgtaatta cgattattaa cctcttctta taaaggagag gtttttttat
    22321 tttgagtaaa tttctaaaat taaaaataag gtaaaaccta taataaaaat taagaataaa
    22381 taagggggaa taaaaatggc aagacattta tttacgtcag aatcagttac agaaggacat
    22441 cctgataaga tatgtgacca aatatcagat tcaatattag atgcattatt agaaaaagac
    22501 ccacaatcta gagtagcttg tgaaactaca gttactactg gtttagtttt agtagcagga
    22561 gaaataagta catctgctta tgtagacatt cctaaattag taagagaaac agtaagagaa
    22621 attggatata caagagcaaa atatggattt gattgtgata cttgcgcagt tataacttct
    22681 attgatgaac aatctggaga tatagctatg ggagttgatg aaggtctaga aagtaagact
    22741 ggagaagaga tagaagaaga aatagaaaaa gttggagctg gagaccaagg tataatgttt
    22801 ggatttgcat gtaatgagac tccagaatta atgccacttc cgatatcttt ggcacataaa
    22861 ttatcaagaa gacttacaga agttagaaaa acaggtttag ttgattattt aagaccagat
    22921 ggaaaaactc aagttactgt tgaatatgaa ggaagtaaag ctgtaagagt acatacagtt
    22981 cttatatcag ctcaacattg tgaaacagta tcaaatgata aaataagaga agatttaatt
    23041 aatcatgtta ttaaggaagt tataccagca gaattacttg atgaagagac taaaatttat
    23101 ataaatccaa caggaagatt cgttatagga ggaccacaag gagatacagg tcttacagga
    23161 agaaaaataa taatagatac ttatggtgga tattctagac atggtggagg agctttctca
    23221 ggaaaagacc ctacaaaagt tgatagatct gctgcttatg cagctagata tgttgcaaaa
    23281 aatattgttg cggctggtct tgcagacaaa tgtgagatag aacttgcata tgctataggt
    23341 atagctagac cattatctat atttatagat acttttggga caggcaaggt ttcagaagaa
    23401 aaacttgttg aattagtaaa taaacacttt gatttaagac cgggagcgat aataagagac
    23461 ttagatttaa gaaagcctct ttataaaaag gttgctgctt atggtcactt tggtagaact
    23521 gatatagatt taccttggga aagaactgac aaggttgagc agttaagaaa agatgcttta
    23581 ggtgaataat ttttaactaa aaagctgtta gaatgtaatt ttatgattac tttctaacag
    23641 cttttttatt atgagttaaa tatttttaat aaatgttaat ttatcatcat ataatgaata
    23701 aacagtattt aatctggtca atacatttta tataatactc tactaaattt atacatatta
    23761 tgggatatat gttaaagaat ggttaagaat gtgttaacta atgttaaata attaaaaata
    23821 tattgaaata tcacaataat gagtctaata taatcttaat aaacttacac tttaaaaata
    23881 attttaattg ggggggagta gtgtatgtca ggcaaagtaa atattaaaac ggttataagt
    23941 tttgctggag cttatgttgc tactgtaata ggttctggat ttgcaactgg tcaagaaata
    24001 ctgcaatttt ttacgtttta tggatatgcg ggtattatag gtggaataat ctctatggta
    24061 ctattttcat ggtttggagc agaagttata gataagggaa gagaattgaa acttaaggag
    24121 ccaataaaaa tttatcaagt atattgtgga aaatatcttg gaacattttt tgaatggttt
    24181 ggtccattgt ttttatttgg aacattagta ataatgattg caggagcagg agctacatta
    24241 tcagagtact atggattaaa tccatatgta ggtaggattg gtatggcaat cgtttcccta
    24301 ataactgttt cattaggttt gacaaggctt tctaaaatac taggtaatat aggacctata
    24361 ataataatat ttacactttt agtaggagca attagtttat ttagcaatat tgatgcttta
    24421 tcaaatgctg gaaatatggt tgatagttta aatattaaaa ctgctacatc aaatggatat
    24481 ttctcgggag tactttatac ttgttacaat gtaataatag taataacatt cttgacagga
    24541 atgggagctt ctgcagtaaa taagaaagat gctgtatggg gtggtatagt aggtggagta
    24601 gcacttatgg cagcagctat aatgatgaat ttggcattac tttcagacat aggaaatata
    24661 tacacaaaag agataccagc actttactta gctgataaga tatctccagt aataggtatt
    24721 ttattctcag tagtactttt actaggaata tacactacag ctgtaccatt gttatggtca
    24781 gtaacaaacc gatttgtaga agatgaccac cctaagttta aaattataac aatagtggta
    24841 agtatattag cttgtatagg aggtttactg ccatttgata agttagtagg tacactatat
    24901 ccatatacag gttacatggg aattttaatt ttactatgta tgctttatag aagaattact
    24961 aaaactgagg ggtacaagga gaataaatct gaaataagtt agtactgtaa tatacttatg
    25021 aaatttaatt tatactctaa atatagagta attaaaataa aatagctatt taaatatggt
    25081 acctgtattt catggtacca tattttatgg atgcagattt tttttgtttc tgataaaatg
    25141 tataatagtt ggaaattacg ttaagtataa aaatataaaa cttttctacg attatcatgg
    25201 ttttaatata taaaattata ctaacattta aatggagcga ttattagtat ttttaggaga
    25261 taaaaatgga aaaattagaa ggaatgataa gtgaaatagt ctttaaaaac gaagacaatg
    25321 gttatacaat agctcacctg gtaaatgaaa atgatgaaat tgtggttgta ggatgtatgc
    25381 ctactttggc aattggagaa agcatagaag ttgaaggaaa gtgggtaaat cataagattt
    25441 atgggactca atttgaagtg aatagtttta tgccagtaac accatcatcc ttagaaggaa
    25501 tttatgttta cttatcttct ggaatgatac atggtattgg tgaaaaaatg gcaaaaagaa
    25561 taattgataa gtttggtgta gatacgttag aggtgataca aaattcacca gaaaaactac
    25621 aagaagtaga aggtataggg agtaaaaaag taaaacaaat agtaaaaagt tatgaagaag
    25681 acagagaatt gagaaatata ataatacagc tatctccttt tggaataaca ccaaattact
    25741 gcctaaaaat atataaaaaa tataaaagca gtgcaataga agttataaat aaaaacccat
    25801 accaacttgc tgaagatata agaggaattg gatttaaagt agctgatagt atagcaagta
    25861 aaattggtat agataaaaac tctaaagata gaatttgtca gggaatctta tacactttaa
    25921 ataaatcttt aagtaatggg catacatatt taccagagca tgttctaata caagattcgg
    25981 aaaaattgtt ggagctaaat ggagaaatac taaaagagtg tgtaatgatg ctagtttata
    26041 atcaaaaaat acatatagag aaagtaaaca atgaaaatct tatatacctt atgccatact
    26101 atttagctga aaatggagta tgtagtcaaa tagtaaaatt atcacagtat gaatttgaag
    26161 acttaaaaat agatatagat aacgagatta atgttttaga agaagataag aaaattaagt
    26221 tagcagaaaa acaaatttta gcagtcaaag aatctgttaa tagtggagta ttaattataa
    26281 ctggtggacc aggaactggt aaaacaacta ccattaatgc tatcatagat atatttgaaa
    26341 ataatggtaa aagtgtgact ttggcagctc caacgggaag agcagcaaag agaatgagtg
    26401 aaacttctaa taaagaagca aaaactatac ataggttgct agaaatggga ttttcaacag
    26461 atgatgattt aacttttttt aaggatgaag aagacccaat aaattcagat gtaataattg
    26521 ttgatgaggt atcaatggta gacattatct taatgtacaa tttacttaga gctataaagt
    26581 taggaacaag agtaatttta gttggagata gtgaccaatt accttctgta ggtgctggaa
    26641 atgtacttaa ggatatgatt aattctaata ttataaatgt agttaaatta aatgaaatat
    26701 ttagacaagc gcaagaaagt atgataattg taaatgccca taagataaac aatggagaac
    26761 cactttatct aaatacaaaa ggtaaagatt tcttttttat aagaaaatca actaatgaag
    26821 aaattttaaa tgaaataata ggattggtta atgaaagatt acctaaattt tataaagttg
    26881 ataaattaaa agatatacaa gttttatctt caatgagaaa aggtgaattg ggagttacta
    26941 atctcaatat agagttacag aagtatctta acaaaaaaga aaaatttaaa gttgaagaaa
    27001 gtttttctaa aagattattt agagttggcg ataaggtaat gcaagtaaaa aataattata
    27061 caaaaaaatg ggaaacagaa gaccaaaaag aaagtggaga aggtatctat aatggagata
    27121 taggatatgt ttatcacata gataaagata agaaaactat ttatatatta tttgaccaga
    27181 ctaaaattgt atcataccta tatgatgaat tagatgaaat tgaccatagt ttttgtacaa
    27241 caatacataa aagccaggga agtgaatttc cagttgtagt attgccaata gcgtgggctc
    27301 ctcctatgtt acttagccgt aatttattat atacagcagt aacaagagca aaaaaattag
    27361 ttgtattagt tggagatgtg aaatatcttg aatatatgat aaaaaataat agagttaatc
    27421 aaaggtattc aaatttagga tataagctaa ataaatttaa acaagaaggg ttgttgatag
    27481 aataaatatg aaaatatgca atgataattt tattaagaat tttaaaaata tgattaataa
    27541 atgtttggat tttatatatc ctgagaatat aagttgcata atttgtgata aatccataaa
    27601 gaaaacaaat acttattcaa tatgtaaaag ttgttttaaa gaaatgaatt ttatacaaga
    27661 tggttgcatg aagtgtggaa aaccaataat acgtcattca attgaaaaag aatttattga
    27721 agagtgtagt tattgtttta ataaagactt ttattttgat aaatcaatat cttgtattga
    27781 atacaatgat gtgacaaaaa agatggtgtt agggctgaag tataaccaaa aaacctttat
    27841 ggctaagtat atagctcaaa taatgaaaga aaaattatat ttagaaaaca taaaatttga
    27901 ttatatatta tttgttccac ttcataaaaa aaggctaaat aaaagaggtt ttaatcaggc
    27961 acaaaaaata gcatttaatt taagtaaaat agtaaatatt ccattattag attgcataag
    28021 tagaaaaaaa tatacaagaa tgctatataa attgaataag aaagaacgaa aagaagaatt
    28081 aaaaaatgta tttgttgtta aagaaaatgt taaattaata aataataaaa atatactttt
    28141 aatagatgat atatttacta ctggtctaac aacaaacgaa atttctaaac ttttaaagtt
    28201 atcaggtgct aataaggttt ttgtattgac tctacttaca aaagcaaatg ataattatat
    28261 tatggaataa tcttatatat tgacaatttt agtttaaaat attaaaatga ataagtgata
    28321 tttaggtctg tggttgaaag tccaagccag tcgcaggcaa agggatccac gtaagttaac
    28381 actaaaaatt gggttaatga tcatggtgcg gcttagaagt aagacctacc gataataaaa
    28441 tcgagagaga gtagtagtgc ggcgaaccca agcgaaccct ccagtaggcg agtgtggggt
    28501 caaaaaccag gtcagctaaa tatcatatta aaagagagct agcatacctt tatgtatagc
    28561 tcttttttat atataataaa tttaggttga gtcataaaga ggtgatatgt ttgaataaaa
    28621 gacaagaaaa tttattaaca acattgctat taaaagaaga gtttataaat gttaataaga
    28681 ttgccaatga ttttgagtgt tcagaaagga caataagaaa tgattgcaaa tatattgatg
    28741 aatggttatc tactttttgt aatgcttata tcaaaagaaa acctaatatt gggattaaat
    28801 ttagtggaga tggaacagac aaaatactgg taaatcagaa gttgcgaggt gaagttaaaa
    28861 gagtaaatag tgagcttcat aagcaagttg agatattaaa tttactactt tgtaataata
    28921 aaaaagttac tttaaatgat ttatctaata agttgtacat aaataaaaat attgtcagag
    28981 aagaaataag taaattatca gtagcattaa ataattataa tttaaatatc tcgactaaaa
    29041 aaggcagtgg tatatatatt caaggggatg agaaagacat aagaataatg ttagtatcat
    29101 tcttatttaa atatatagat aaatacagat taaacataga tgaaatagag tacttccata
    29161 tagtagatat aataattgta aaaaacataa tgcttactat agaggaggac atggatataa
    29221 gacttacaga tatatcattc aaacaattga tgctattttt gcttataaat ataatacgaa
    29281 taaggctggg aaattcttta aaagtatcaa atactaagaa agagcttaag ctaaaaatgc
    29341 ttatattgga tatagaagat gaattgaaat ctaatttatc catagtatta aattatgaag
    29401 aaaaattgtt tatagaatct ttaatattgg gtatgaacaa acaaataaat agtaataata
    29461 ttaagaaatc tttatatcta aatgaagaat tagcaagtta tacaaaaaaa attataaatt
    29521 tagtatcaga agaatcagga ataaatttta ataatgatat attactttat gaacaattaa
    29581 tatgtcattt gaatgttaca atgcatcaaa tgaaaagcaa tgtttactta gaaaatccac
    29641 ttttagataa cataaaaact aaattttgtt ttttatttac agtaatatca agttcaatag
    29701 aaattgaatt aaaaggaaaa caaataacgg aagatgaaat tggttatctt acacttcact
    29761 ttcaaacctc tctagaaaga aaatacagca aaagggaatc aaataaaaag gcaagtattg
    29821 tatgtccatt tagttttgga gtaagtatgc tattaaaagt aaagatagaa aagagatttg
    29881 ataatataaa aattattgaa acattaagag aggaagattt aaaaagagat aacttcaata
    29941 agactataga ttttataatt gcatttcaag agtatgaaaa tatccaaaaa ccaattttta
    30001 taacaacacc attatttact gatgaggatg agaataaact taaagagttc acaaacaaaa
    30061 taaaagaaaa agacacttca tataaactta tgaacagatt aatgatgtct gattatttaa
    30121 taagagagtt agaacatgat gatatttatg aagctataac atatttaact aatttattaa
    30181 ttgaaaaaca ttatgcagaa aaagagtatt tacaatctat tataacaaga gaaaagtcat
    30241 atccaaccaa tgttggaaaa ggaatattat tccctcatgg agacatgaaa tatataaaac
    30301 aatctgtttt atgttttgca aggttaaaaa caccaataaa aattagaaat ggaaaggata
    30361 taaagattat acttttgtta gcatataaaa aagatgatga tagaaaagta tttagagaat
    30421 tatttaaaga aatatctaat ttaacagagg atgaaaatat gctagatatt ctaagtaaat
    30481 gtgacattga aaaggtaaaa gatattctta tataaaattc atagtgaact atggataagc
    30541 acaaaatttt ccatgtgatg gaaaaagttg tgcatgtttt tttgttttta taagatatta
    30601 tatgtttgta aggtataaat aatctaaaag tagagaagca acttaggagg aaaaaatgga
    30661 agaaatagtt ttaaagatta taattcatgc tggaaatgca aaatctatgc tttatgaagc
    30721 acttgactat gcaaaggaaa acgattttaa aaaagcagat gaattaattg aaaatgcaaa
    30781 tgaagaaatt ttaaaagcac ataaagtaca aactgaactt atacaaaagg aagctggggg
    30841 agataaatca gatatatcta ttttattgat tcactctcaa gaccatttaa tgacttgtat
    30901 gagtgagaga aaccttataa aagaaatgat tatgttaaga aaagaaattc aaaaattaaa
    30961 ataaaaaaat taagggggat ttaaaaaatg aaaagaaaag tatatttatt ttgtagcttt
    31021 ggtatgtcaa caagtttact tgcagataaa atgcagaagg tagcagatga acacaattta
    31081 ccaatagaag tagaggcatt tccgatagca gaaattgaca aaatagttga agaaaaaaat
    31141 ccagattgta tattattagg accacaagtt aaatacatgc taaaggagtt aaaaccaaag
    31201 tttgaagctc aaggaaaact tatagatata atcaatgaag ttgattatgg aactatgaat
    31261 ggggaaaaag tattaaagct tgctataaag ctaataaaat ctaaacaagc gtagaattat
    31321 agtaaaaaat aataggaaat gagggtggat agatgaatag attggaaaga atacttatgc
    31381 cattggctga aaaaataggt aaaaataaat tattaattgc aattagagat ggttttttag
    31441 tatcatcacc attattaata ataggttcat tatttttatt agtagcaaat tttcctataa
    31501 aaggatggaa tgaatttgta ggtcaaattt ttggtccaga ttgggctgta aaattgcaac
    31561 aaccaactgt agcaacattt gaagtaatga ctctacttgc agttttaggg attggatatt
    31621 cttatgcaaa gcaaatagat gtagacccaa tagcatcagc agcagttgca atagttgcct
    31681 tttttataat aacaccattt gtaataccat atacaccaga aggaacggaa tcagtttaca
    31741 tggtaacagg tataccacta ggttggatgg gttctaaggg aatgtttgta ggtatgataa
    31801 cagcaataac ctctgtaaaa ttatttgctg cagttgtaaa aaaaggatgg acattgaaaa
    31861 tgccagatgg agttcctcca actgtatcaa aatcatttgc agcattaata ccaagtgcaa
    31921 tagttatggt agttttcttt ttagttaaga tagcttttga agctacacca tatggaagta
    31981 tacatgaatt tatatttaag tttttacaaa tgccattatt aaaattagga aatagtttag
    32041 gtgctatggc aatcgcttat atattcttac atttattctg gttctttgga ataaatggaa
    32101 gtagtgttgt tggggctgtg tataatccaa tattaaaaat tctatcagct gaaaacttag
    32161 ctgcttttca agctggttca aaaataccaa acataataac aggtcaattc caagatatgt
    32221 ttgcgacatt tggtggagca ggtagtacac tatcattagt catagctatg ttattaatat
    32281 gtaaaagtaa acgtataaag tcaatgggaa aactatcaat tttaccagga atatttggta
    32341 taaatgagcc attgatattt ggtctaccaa taatgcttaa cccaatacta ttgataccat
    32401 ttgcaatagt tccaacaata aatataataa tagcttactt ctgtatgtca gcaggattag
    32461 tgcctcttac aaatggagtt cagttaccgt ggacaactcc aattatattc tctggttttt
    32521 taacaacagg atggcaaggt gcagtcttac aattagtact tcttatacta ggaatattta
    32581 tgtatatacc atttataaaa atgctagata agcaatactt gagagaagaa aatgaaactg
    32641 ttgaagagga agatgatgat atatcttttg atgatttagt attataggag gaagattatg
    32701 aagaaaatag ttatgatatt ggaccaaata caagctggag ctggaggaaa agagaaaagt
    32761 aatatacctc ctgctggaaa atcaagccca ttaggaccag gtgtaatgat ggagcaattt
    32821 ttaaatgaat ctaaggtaat agctacctta ttttgtgggg atgaattttt tgtaaataat
    32881 caagaagaag taacgagtaa gatgatagct atggtaaaaa aattaaaccc tgatgtagta
    32941 atctgtggac caagctttaa ttacgaaaac ttttctaaga tgtcagcaat actgtctaaa
    33001 aatataaatg ataaaacaga tataccagct tttgcagcta tgtcagaaga aaatatagat
    33061 gtgataaacg aatataaaaa tgacatatgt atagttaaga ctccaaaaaa aggtggaatt
    33121 ggtcttactg attcgttaaa taacatatgt aaattggcaa aagcaatagc aaataaagaa
    33181 gatataactt tgatgaaaga agaattttgt tattaataag agtattaatt attagcaagt
    33241 ttaaatatta tactatttat aataagcata cattagtaaa atctatataa tttaaggagg
    33301 ggaattttaa catgtgggga atgatagcga catggagaat ggccctagaa ggaataactg
    33361 aggcatcaga acatttagaa aaaggcatgg aagcaggtga tgctgtagag cttgctgtaa
    33421 gaagggttga agactatcca ttctataaat cggttggata tggtggactt ccaaatgaaa
    33481 attgtgaggt agaacttgac gctgcattta tggatggtaa cacattagat attggcgcag
    33541 ttgctggtat aagagattat tctaatcctg tcagcatagc taggaagtta agttatgaga
    33601 aggtaaataa ttttttagtt ggaataggtg ctgaagatta tgctcataaa aatggatttg
    33661 aaagaaaaaa tatgttgact gatagagcga agttacatta taaaaagaga aaaaaagaga
    33721 ctttagacaa aggattaagc ccatatgctg gtcatgatac tgttggtatg atatctttag
    33781 acaaaactgg aaaaatgtgt gtagctacat caacaagtgg attgtttatg aagaaaagag
    33841 gtagaatagg agattctgca ctatctggtt caggattcta tgttgatagt agtataggag
    33901 gagctacagc tactggtttg ggagaagatt taatgaaggg atgtatatct tatgagatag
    33961 ttagacttat gaaagaaggg aagcatccac aagaagcatg tgatattgca gtaaatgaac
    34021 ttgataaaca gcttatcaaa agaaggggaa aagctggtga tttatcatta gttgccatga
    34081 ataataaagg tgaatgggga gtagctacaa atatagctga ttttactttt tcagtagtaa
    34141 ctaaagaaga atctcctact gtatatattt caaatagagt agatggaaag actatacata
    34201 aggttatatc aaatgagtgg atggaagcat atgaggctag aataaaagca ccaatagaag
    34261 taagtgagta aagtagtggt tacataatta aaatagagtt ttataataag gagattttta
    34321 tgaatatcat aagtaataaa atagatgagt taaaagaaga tttattaagt gatattatag
    34381 atattgtaaa aatacctagt gtcaaagggg agtcagaaaa tggcttccct tttggcgaaa
    34441 aagtagggga ggcacttaat aaagcattag aaatatcaga aaaattggga tttaaggtta
    34501 gaaacttgga taattatatt ggttatgcag agcatggaga tagtgatgat tatgtttgtg
    34561 taattggaca tgtagatgtt gtacatgaag gagatggttg gaagcatcag ccttataagg
    34621 gagaagaaac taatggaaga atttatggac gaggagtact agacaataaa ggacctataa
    34681 tgtctgcctt atatggatta tatgctatta aagaattaaa cttaaaatta gataagagag
    34741 ttagaataat atttggtaca aatgaagaaa gtggatttga agatatacca tattatttag
    34801 aaaaagaaaa agcacctatt atgggattta caccagattg taaatatcct gttgtatatg
    34861 gtgaaaaagg catggcaaag attagaataa aaagtaaaat caattatgaa gaagatgtat
    34921 acctaggttt tatagaaaat atgtctgaaa atgtgcttgt tacatataaa gaactgaata
    34981 tagaaaatag tgatactata ttagatataa aggtaaaata cgattttagt tataaattga
    35041 aagatgtatt agatgaaata aaagctagtt ttccaaatag tatcgatata gaggtaattt
    35101 ctaattttaa tcctgtatat tttgataaag agagtaatct tgttaaaaag cttcagctag
    35161 cttatgaaag ggttacatca ttagatggaa ctccagtaac aactaatgga ggtacgtatg
    35221 caaaagtaat gccaaatata gttccgtttg gaccttcatt tccaggacaa aaaggaatag
    35281 ctcataatcc tgatgaatac atggatatag aagatataat attaaatgca aagatatttg
    35341 caaatgcaat atatgaatta gcaaaggagt agttatgtta gaaataatag gaatgtcagt
    35401 agaagacgct aaaattatag aagattgtgg tgcagataga atagagttag ttagtgcttt
    35461 aactgaaggt ggattgacac caagttttgg tcttatagaa agtgttgtta acagtgtaaa
    35521 gatacctgta aacgtaatga taagacatca tgcgaaaagt tttgtataca gtaaagaaga
    35581 tataagtata atgcaaaaag atataagtgt ggttaaagag attggtgcta atggagttgt
    35641 atttggtgta ttggataaaa acaacaatat agatgagaaa aatctaaatg ttttattgaa
    35701 atgttgtgat aatcttgatg ttacatttca tagagccata gatgaatcaa atacaataga
    35761 tagtgttaaa attttgaagg attatgataa aattacgaat atattaacat caggtgggaa
    35821 aggaagtata gttcataaca tacaaatgat aaaaaatatg atgcttagtt ctaatcacat
    35881 aaaaattctt ttaggtggag gtcttaattt taataatata gaaaagataa aggaattaac
    35941 taaggcaagt aattttcatt ttggtacagc gattagaata aataacagtc cttttgaaga
    36001 tatagataga caaaagctta aacaactcgt aaatataatt tcaagatagt atatgaatat
    36061 aaaatactta gtttaaataa agagagtaag ttaattataa tttgggtata tgaatgttaa
    36121 taggtattaa atatgaaatt attgttgagt atgctttata acttttaagt agttttaaat
    36181 ataaataatg aaagaaaaga tagattatgg aggttttaga gggtttatat ttattaaaaa
    36241 attataaagg tatattggca tataatacac ataatttcct aaaattacca tcttatgaca
    36301 tgaattcaca tattaataaa caattaatgg agatttttgt ggatagaaat aggagtaaaa
    36361 atagctttaa ataaacaaaa acaagagtta ttaacagttt tatccacatt atccacaggt
    36421 gtttgtaata atattaatcc acataagtat caatgtaact aaaaatgata aataattcta
    36481 tcaattgaat agaaaaaatc ataaaagtaa ataataaaaa gaggattatg agagttcgtg
    36541 tagaatatat tattaaagta taaattttct tatactggca taattgaagg gagatgtatt
    36601 tatgaacata attatatctg gaaaacaaat cgaattaact gatgcaataa aaaataaaat
    36661 cgaaactaaa ctaagtaagt tagataatta cattcatcca gacactgatg taaaagttac
    36721 agtaagtgcg agaaaagcaa gacaaaaaat agaagtaaca atagctactg taaatggtcc
    36781 tataattaga gcagaagata tgcaggaaaa tttatacaca gctatagatg ttgtatatga
    36841 taaattaagt aaacaattaa aaaaatataa aaagagatta caagataaac ataaagacaa
    36901 caagagtata agattccaag atgcagagat aaatctagct gatgaacaag aggattatgg
    36961 ggatgaaaat caagaattag taattcaaag acgtaagaaa tttagtgtta agccaatgag
    37021 tgaagaagaa gctgtattac agatggaatt aataggacat gatttttata tgttcaagaa
    37081 catagattca gatgaaatag cagtagttta taagcgtaat aatggtggat atggtataat
    37141 agaacatgaa taaagcaatt tagatttaat catattagct atcgataaat aaattatcga
    37201 tagcttttta aattaatatt aaaagtatag tttttatggt aaaatgaata atagtacaat
    37261 aaagaacgag gagagatgta aaatatgagc tttatggata atttattcaa catggcagat
    37321 aaaaaagaat taaaaaagtt caataaaact gttgatataa tagattcatt agaacctaaa
    37381 tttgaatcta tggcagactc ggaacttaaa aatatgacaa atatatttaa agaaagatta
    37441 gcaaatggtg aaagtataga tgatatactt ccagaagctt ttgcagtagt tagagaagtt
    37501 tctaagagag tgttaggact tagacactac agagttcaaa tgattggagg tatagtacta
    37561 catcaaggaa gaatagcaga aatgaaaaca ggtgaaggta aaactttggt tgcaactgct
    37621 cctgtttatt tgaatgcgct tactggaaaa ggtgtacatg ttgtaacagt aaatgattac
    37681 cttgcaaaac gtgatagaga ccaaatggca aaaatctatg agtttttagg aatgagtgta
    37741 ggcgttatta tacatggtca aaatccaaaa gttagaaaag aacaatatga ttgtgatata
    37801 acttatggta caaataatga atatggattt gactatctaa aggacaatat ggtaatacac
    37861 aaagagcaaa gagttcaaag aggattaaat tatgctatag tggatgaggt tgactctata
    37921 ctaatagatg aggctagaac accacttatc atatctggac ctggagacaa atcaactcac
    37981 ttatattcag atgcaaacac atttgtttta accttaaaac cagatgatta tgaattagaa
    38041 gaaaaagata aggctgtatc tctaacagca tcgggtatac aaaaagcaga agtgtatttt
    38101 aatgttgata atataacaga tatatctcat acagaattat accatcatat aaatcaagct
    38161 ttaagagcac atgtaataat gaagaaagat gttgattatg ttgctaaaga tggagaaata
    38221 gtaatagtag atgagttcac aggtagactt atgtttggta gaagatattc agaaggttta
    38281 catcaagcaa tagaggctaa agaaggtctt aaaatacaaa gagaatctaa aacactagct
    38341 actgtaacat tccaaaatta ctttagaatg tacaaaaaac tttctggtat gactggtaca
    38401 gcaaaaacag aagaagaaga gtttaaagca atctacaaaa tggatgtttt ccaagtacct
    38461 actaataagc ttatgataag ggaagactta ccagattgtg tatacaaaag tgaaataggt
    38521 aaatttaatg ctgtagcaca agaaataatt gaaaggcaca aagtcaatca accaatactt
    38581 gtaggtacag tatcaataga aaaatcagaa ttattatcac aaatactaaa gaaaaagggc
    38641 ataaagcacg aggtattaaa tgctaagcat catgataaag aggcagaaat aatagcacaa
    38701 gctggtagac ttggagctgt tacaatagct actaatatgg ctggtcgtgg tacagatata
    38761 gttctaggtg gaaatccaga ctttttaact aagagagaaa tgagaagaaa tggatttaaa
    38821 gaagaaatag taaatagagt agatactcca atagaaggta tacctgtaaa aggtaatgaa
    38881 attctatttg aagctagaga agagtatgaa aaactatttg aaaagtttaa gcaacaaaca
    38941 caagaagaac aaaaacaagt tgtagaagca ggtggacttg ctataatcgg tacagaaaga
    39001 cacgaatctc gaagaataga taaccagttg agaggtcgtg ctggacgtca aggtgaccca
    39061 gggagttcaa gattctatat aggtcttgat gatgacctta tgagattgtt tggtagtgat
    39121 agaatatctg gtattgtaga taaaataggt cttgaagaag acatgccgat agaacataga
    39181 atattatcta agtctattga aggagcacaa aagaaagtcg aaggtaaaaa ctttggaata
    39241 agaaagcatg tacttcaata tgatgatgtt atgaataaac aaagagaaat aatatatgca
    39301 gagagaaaac gtgtattaga aggtgaagat ttacaagaac aaatacaatc aatgactcat
    39361 tctataatag aagaagcagt tactctttat acacaagata aaggatttga tgaagaagga
    39421 ttcaaagaac atatgtataa tctgttcttg ccaaaaggta gcatagaaat accagaaata
    39481 gaaaaactta atccagttga aataacagaa aaagtttatg aaatagctat gaagatatat
    39541 acttcaaaag aagaacaagt tggctatgag agaatgagag aagttgagag agtaatctta
    39601 cttcaagctg ttgataacca ttggatagac catatagatg ctatggacca attacgccaa
    39661 ggtattggtc ttcgtgctgt aggtcagcaa gacccagtta ttgcttataa aatggaaggt
    39721 tttgatatgt ttgatgaaat gaataaacat atcaaagaag atactgtaag atatttattt
    39781 aatataacaa tagaaactcc agtggaaaga aaagcagttg tagatgttga aaatctatct
    39841 tctccatcag atggaacact tccaacaagt aaaactgtta aaaaagatga aaaggttgga
    39901 agaaatgact tatgcccttg tggaagtggt aaaaaatata aaaactgttg tggaagataa
    39961 tctcggagga ttaaatgtta aagttacaag agtatagaca tagtatggag cagatggctt
    40021 caaatttaga agaattgagg gtttctcttt gacatagctt cattgatgaa aaatatacaa
    40081 attaatgaag aaaagatgaa tcaacaagac ttttggaatg ataatgaagt cgctcaaaga
    40141 gtacttcaag aaaataaatc tttaaaagag acattagaag aatatgaaag cttaaaaagc
    40201 ttattagaag atatagaagt attaattgaa ataggtttag aagaagatga tgattctgta
    40261 gaaagagata tagaaaagtc catagaatca atggaagaaa aattatctga aatgaaaata
    40321 aagactcttc taaatggaga gtatgataaa aataatgcta tattatctat aaatgctggt
    40381 actggtggct tagatgctca agattgggct caaatgcttt tgagaatgta tataagatgg
    40441 tcagaaagta agggttataa agttaagctt ttagatataa tatcagaccc tgaagcaggg
    40501 ataaagacag ctacaattct tgtagaagga acaaatgctt atgggtattt aaagagtgaa
    40561 aaaggagttc atagattagt gagaatatct ccatttgacc catcaggaaa aagacatact
    40621 tcatttgcat ctatagatgt aacaccagag ttagatgaaa atatagaagt agaaattaat
    40681 ccatcagatt taaagataga cacatataga gcttctggag ctggagggca acatgtaaat
    40741 acgactgatt cagcagttag aataacacat atccctactg gtgtagtagt tcagtgccaa
    40801 aatgaaaggt ctcagcatct aaataaggac agagctatga gacttttaat ggcgaaattg
    40861 atagaattaa aagagttaga gcaaaaggaa aaaatagaag atatacaagg caaatactca
    40921 caaataactt ggggtagtca aattaggtca tatgtttttc aaccatataa attggttaaa
    40981 gaccatagaa caaatgctga gtttggtaat gtagatagtg taatgaatgg aaatatagac
    41041 ttgtttatta atgaatactt aaaaatgaat aaaatagttt aataaatatg cactgtaatt
    41101 aagatatatg tataagaagg ttagtgctta atatgcactt acagcactta ggctgaggac
    41161 gataaccctc agccttcgtt ctgttttgtt atacttatga aaaactaaca ttaggagtga
    41221 ataaattgga tataaatcaa atcttaaaaa aagagtttaa tcttagagat gaacaaataa
    41281 ataatacatt aaaacttatt gatgaaggaa atacaatacc atttatagct agatatagaa
    41341 aagaaatgac tggcgaaatg agtgatgtta cactaagaga attttatgaa aaattaatgt
    41401 atttgagaaa tcttcaaagt agaaaagatg atgtagttag actaatagat gaacaaggga
    41461 aattaacaga tgaaataaca caaaatatag aaaaagcaaa aacattacaa gaagtagaag
    41521 atatatatgc tccatataaa caaaagaaaa gaactagagc aacaatagca aaagaaaaag
    41581 gattagaaaa tttagcattg tcaatactag agaataattt agataatata gaaatagagg
    41641 caaagaatta tcttgatgaa gagaaagaag ttctttctat tgaagatgct ctaaaaggtg
    41701 ctagagatat aattgcagaa ctagtctcag atgatgctaa gattagaaag tatataagag
    41761 aattagcttt aagagaaggt atgatagtta gcaaaagtgc tactgatgag aaatcggttt
    41821 atgatatgta ttatgattat agcgaagcag taaaaagtat ggctccacat agagttttgg
    41881 caataaacag aggagaaaaa gaaagcttcc taaaggttaa gttggaaata aataatgata
    41941 aagttttaaa ttacataata aacgaatatg taaatgataa aaatttcaaa aataaagaag
    42001 aaatcgtaag ttcaatagaa gattcttata aaagattaat tttcccatca atagaaagag
    42061 aaataagaaa tcatttaaca gaaatagctc aagaaagagc tataagtgta tttggaaaga
    42121 atgttaaaag tttattgctt caaccaccag ttaaagacaa agtagttatg ggatttgacc
    42181 ctgcatttag aactggatgt aaaatagctg tagtagataa aaatggtaaa ctactagatt
    42241 atacaacagt atatccaaca gaccctcaaa atgatgtaga gggagcaaag aaagttttaa
    42301 aaggattgat tgaaaaatac gatatagata taatttcaat tggaaatggt actgcatcta
    42361 gagaatcaga aacttttgta tcagaaatga taaaagaaat agatagcgaa gtgcagtatg
    42421 tgatagttag tgaagcagga gcatctgttt attcagcatc tgaacttgca aatgaggaac
    42481 atccagatat aaatgtatca ataagaggag ctatatctat agctagaaga ttacaagacc
    42541 cattagcaga gcttgtaaaa atagacccta aaagtattgg tgttggtcaa tatcagcatg
    42601 atttaaacaa aaagagatta gaagaagtac tagatggagt tgttgaagat tctgtaaata
    42661 gtgttggagt agatttaaat actgcatcat actccctact agaacatgtt gcaggaataa
    42721 gtaaagctat agcaaaaaat ataatagcat atagagaaga aaatggagat tttacgtcaa
    42781 gagcacaatt gaaaaaagta aaaagactag gacctcaagc atttactcaa tgtgctggat
    42841 ttatgagaat cttagagggt aaaaatccac tagataatac aggtgtgcat cctgaaagtt
    42901 atgatatatg taaaaaaatg attgagataa ttggatattc tttagatgat gtaaagaata
    42961 agaatatagg tgaaatagat gaaaaaataa aagaaattgg cttgagggaa ttaagtgaaa
    43021 aactggaagt aggacaagtt acattaaaag atattatagc tgaaatcaaa aaaccaggaa
    43081 gagacccaag agaagaaggt ataaaaccta tacttagaac tgatgtctta aaaattgaag
    43141 atatacaaga gggtatgact ttaaaaggta caataagaaa tgttgtagac tttggtgcat
    43201 ttgtagatat tggaataaaa aatgacggat tagttcataa atcagaaatg agtaatagtt
    43261 ttgtgaaaga ccctatgagt attgttacag taggtgatat agtagatgta aaagtcattg
    43321 gaattgactt aaacaaaaaa agagttgcct tatcaatgaa aaaatagata agtttatgta
    43381 tggttatagt acttagattc tatatttgta aatgagatta ctcgagtgta taaatgttaa
    43441 aaatttgtaa aagtatatag taagaaaata tattaaaata agaacgatag tcttgaaaat
    43501 caggaggaaa atgaatgatt tttataaaaa atggtaaaat aaataccata acaaatggga
    43561 taattcatgg agatatactt atagatgaag gaaaaataat agaaattgga gaagatttga
    43621 tagctccttt agatgtagag gtaatagatg ctagtaataa acttgtgttt cctggattta
    43681 tagatgctca tactcactta ggcttatggg aagatggtat agggtttgaa ggagcagatg
    43741 gaaatgaaga gactgaccca ataacaccac aattaaatcc tatagatgga attaatccaa
    43801 tggatagaac ttttaaagaa gcttttgaag gtggaataac ttctgtttgt acaacccctg
    43861 gaagtgcaaa tgttatgggt ggtcaatgta tagctataaa gacatgtgga aaaagaattg
    43921 ataaaatggt aataaaaaat ccagttgcat ctaaaattgc ttttggagaa aatccaaaga
    43981 gctgttatgg tcaggatgat aaatctcctc aaactagaat ggctatagct gctttactta
    44041 gagaaaacct aaaaaaagca gaagaatatt tagaggatat tgatatgtat gagagtcatg
    44101 atgatgaaga ctgtgaaaaa cctgaatatg atataaaaat ggaaagttta ataccagttt
    44161 taagaagaga aattcctttt aaggcacatg ctcatagagc agatgatatg tttacagcaa
    44221 taagaattgc aaaagaattt aatttaaagc ttacattaga ccattgtaca gaaggtcatc
    44281 ttattgtaga tgaattagtg gaagaagagt ttcctgtaat agtagggcca tctttgtcag
    44341 aaaggtctaa atttgagctt agaaatttga catttaatac agcaggaata ctttcaaatg
    44401 caggtcttga tgtatgtata atgacagacc atccagtaat accagttcaa tatcttccaa
    44461 tatgtgcagg tattgcagta aaacatggta tgaaagaaga aaaagcaata gaatcaatta
    44521 cgataaatcc agctaaggct ttaggaatag aagatagagt aggctctata gaagttggta
    44581 aagatgcaga cctagtaatt tgggataatt ctccactaga gattcaaagt aatgtacttt
    44641 acactataat aaatggcaaa gtagtgtatg agaaaaaata acttgacaat tattaaaaaa
    44701 ttaactaata taagagtgta aggaaaatta aaaaatcaaa gaagatcttc ggggcagggt
    44761 gaaattccct accggcggta tagcccgcga actacttagg tggttgattt ggtgagattc
    44821 caaagccgac agtaaagtct ggatgagaga agatatttat ggaagataaa taagtttatt
    44881 ttagtttgtt taaactatat ccataaaaac tcgaagatag ggtcttcgag ttttttgttt
    44941 ttcctaaaaa atataaacat attgattagg gggaaaataa ttatgcaaaa cacagcaaaa
    45001 agaggcaata tgatttcaac aagaacttta gtagtaatgt caattttatc tgcaatatct
    45061 tatttattaa tgtttattca agcaccaata ccaggtatat ttccagattt cttaaaaatt
    45121 gacttatcag atattccagc aatattcgga ggaatgtctc taggaccatt agttggattt
    45181 ggaatagttg tagtaaaaaa tgtgttacaa gcaataacag caacaacaac tggaggaata
    45241 ggagaatttg caaatatatt aattggtggt tcttatgtaa tgatactttg ttattcatat
    45301 aagagaagtg gagatttaaa aggtgtactg attggttttg tattaggaac aatatctatg
    45361 actataattg gttcattagt aaattatttt gtaatgttgc cactttatgg tcaacttatg
    45421 ggactagatg ctataatagg tttaggttca gctataaatc ctcaagttca tgatttattc
    45481 acttttgtaa tttggatgat agctccattt aatgtgctga aggctgttat aatatcttta
    45541 gtaacattgc cactttataa aaaaatggga aatataataa agaaataatt agacaagaaa
    45601 atataaaatt aaatgtattg aatagccata atttatttat aacttcagtc ctatattgtg
    45661 attgatatat taaatatatt atggctttat taatttatca aaaaatgtat ataaaataag
    45721 aaatatttag acaaatttaa attttaaaat aaaggaagaa ttaaaacgat taactattaa
    45781 aatatgaaaa aggtatctac aatatacaga gcgaatttat aataaacaaa aaataaagtt
    45841 attattaaaa aactataaag attaataatc aaacttttaa taataactaa tttaataatg
    45901 ataggggtgt atattcatgc atttatctaa aaaaataaaa aaattatttt tagatgataa
    45961 agggtattat tttaatagta attggaaaga tattcatatt ggtatttatg atacaaaatt
    46021 aggagattct ttaaagtata tcactaatga atatattgaa aatatggaaa gggtaaaata
    46081 cgattatcgt gaattaaaaa atatagaaaa tcaaactgat ggtatgtgta ttttagcaat
    46141 aaggcagaat tacaaggcat tagagtatgt aaaaaatcaa accgaagaat tatgtttaga
    46201 agcaataaag tataattata aagcacttga atatgtaaaa gaacaaacag aatacttgtg
    46261 tcttgaagct ataaaaaaag attgtaatgc acttaagtat gtacgaaata aaacagaagg
    46321 gcttattata aaagcaataa gtcattcttc caatatagat gttgtaagta tactaaaggc
    46381 tttagagact caaacaagaa ggatatgttt agaagcaatt aaaaaagatg gcaggtgtct
    46441 tgcatacgtt agagaacaga gtgaagaatt atgtatagag gccattaagc aaaattataa
    46501 agcattaaaa tatgtaaaaa atcaaacgga aaaaatgtgt atagaatccg taagacaaaa
    46561 tggtatggca ttacaatatg taaataaaca aactgataaa atatgtatag aagctgtcaa
    46621 acaagatgga agaagtatac aatttgtcaa caataagaca gaagaaatat gtataaatgc
    46681 tataagatat ttaaataaaa aatataatat taaagatgta ctaagttaca tagataagta
    46741 tacagaagat atatgtatag agattgtaag acaaaatgga aaaatgctta tgtatataaa
    46801 aaatcaaaca gaaaaaatgt gtattgaagc agtaaaagag aactataagt ctcttaagta
    46861 tgtaaaagag caatctgaga gaatatgtaa agaagcattg aagcaaaacc ataaagcaaa
    46921 agagtatgta aaaattgcta ttgatgattg tatataaggt gtagatacag ccatcaatag
    46981 tgaaagataa aaaaagataa agacaagtac tttataataa tctaggctta tactataatt
    47041 aattaagcag tgatataaat tcacatgacg attatatcaa aatggagatg aattgatggc
    47101 aaaaatatat ttagaaaatg aaaataaaac tagagaaata ggatataaat taggtaaact
    47161 tttaaaagaa ggcagtgtta tctgtttagt aggagattta ggagctggta aaactacaat
    47221 gacacaaagt ttggcagatt cattagggat agaagattat ataacaagtc ctacttttac
    47281 tataataaat gaatatgaag gaaaaatacc tttgtatcat tttgatgttt atagaatagg
    47341 tagtagtgat gaaatgtatg atattggtta tgatgaatat gtaaattcaa atggaatctg
    47401 tataatagaa tgggctaatc taatagaaga tatacttcca aaagaatatt tgaatataga
    47461 attgagatat aaagatgaag gaagagaaat gatattaact ccaaaaggag agttttataa
    47521 agagatagtt gaggagttga ttaaataatg aaaatactag gtatggatac gtcttctatg
    47581 gcagcaagtg tagcagttgt tgaagatgat aacttaatat gtgagtttac agtaaacaac
    47641 aaaaaaactc attcacaaaa gcttatgcca atgatagaaa atatgttgag tatgagtgat
    47701 ttaagtataa aagatatgga tttattagct gtatgtatag gtccaggttc gtttacagga
    47761 cttagaatag gtatggctac agtaaaagct atggcccatg taaataatat accaataata
    47821 gcagtaaatt ctttggaatc tctagcaaat aacataaatt tctgtgatag aaagatatgt
    47881 tgtatattag atgcacagaa aaatcaagtt tattcagcta aatacaagtt tgaaaatgga
    47941 gagatgatag aattagatgg tgttgatgtt gtagagtttg atgctttagt agatgaaatt
    48001 gtatcaacta atgaagaatt tataatagtt ggagaagctg tatacaaata taaagacaag
    48061 ttagagaata taaaaaatat aaaaatacct tcaccagcta ataatgtatc taacgcagga
    48121 agcttatgct ctttagcatt aaataaatat aataaaaata tagatgtaca tacttgttat
    48181 acgataaatc ctatgtatat cagaaaatct caagctgaag tacaatatga tgaaaaaatg
    48241 aagaggctaa ataatggaag ataatacaaa actaatagta gatgatataa aaatagaaga
    48301 aatgactaca gaagatatag atgaagtttt tgaagtagaa aaaaattgtt ttgaagatta
    48361 ttggtcaaaa gagtctttta gaaaagagtt gtctaatgaa gtagcaaggt atatagtagc
    48421 taagttaaat ggaaaaattg tcggatatgt gggaatttgg cttatacttg atgaagggca
    48481 tataaataat gtggctgttc atagtgacta tagagggaaa aaaatagggg acaagttgat
    48541 aaaaggcata gttgatttat gtaaagataa taatattgca tctatgacat tagaagttag
    48601 agcatcaaat aaaatagcac aaaatttata tagaaaatat ggatttaaaa tgggtggaat
    48661 aagaaaagaa tactacagtg ataataaaga agatgcaatt ataatgtgga accaattaaa
    48721 agaggtgtag agtttatgag tgatataata acattagcta tagagagtag ttgtgacgaa
    48781 acagctgcat cagttttaaa aaatggtaga gaaatacttt caaacataat ctctactcaa
    48841 atcgagacac ataaaaaatt tggtggtgta gttccagaag tggcctcaag aaaacatgtg
    48901 gaaaatattg atatagttgt tcaagaagct ttagacaaag caaatatagg ttttaatgat
    48961 atagaccata tcgcagttac atatggacct gggctagtag gtgctttatt ggttggtcta
    49021 tcttatgcta aagcacttgc atatactttg aatattccac ttgtaggagt aaatcatata
    49081 gaagggcatc taagtgcaaa ctatatagag cataaggatt taaaacctcc ttttataaca
    49141 ttaatagtat ctggaggtca tactcacttg gttgaagtaa aagattatgg caagtatgaa
    49201 attttaggaa aaacaagaga tgatgcatct ggagaagcat ttgataagat ttcaagagct
    49261 atgaatttag gctatccagg aggtcctatt attgataatc tggccaagaa tggaaataaa
    49321 catgctatag aatttccaag agcctattta gaagaagata gctatgattt tagttttagt
    49381 ggattaaaat cttctgtatt aaattatcta aatggaaaaa gaatgaaaaa tgaagaaata
    49441 gtagttgaag atgtagcagc atctttccaa gaggctgtag tagaggtttt atctactaaa
    49501 gcactaaaag cagtaaaaga caaaggatac aatatcataa ctctttctgg tggggttgct
    49561 tctaattcgg ggcttagagc taaaataaca gagcttgcta aagacaatgg tattactgtt
    49621 aagtatcctc ctctaatttt atgcacagat aatgcagcta tgatagggtg tgcaggatat
    49681 tataatttta taaatgggaa aacgcatgat atgagtttaa atgcagttcc aaatttaaag
    49741 ataaatcagt agagttagat gaatttgtaa atataaaagt attgtttcaa aaaagtaagt
    49801 agaagtgaaa ttaaatataa taaaaataaa tatagtatat attaaaactt gaagtaaaac
    49861 tatttataat ctttatattc caatgaattg caacaaattt taggatatac agattgtctt
    49921 tggcttagat ttgcttcaag tttatttttt tctatagaaa gattatttta aaaagtgggt
    49981 aatataccct tttttttcat tcttaaaaag ttttatacta attatagaaa gattaaggag
    50041 gggaagaaat gagtcaaagt aaagaagaca aaataagaag tattttggaa gcaaaaaata
    50101 ttaaatcaaa ttttcaaaat aaagaaaatt taagtgaatt taatgaaaaa aaagcaagta
    50161 aaagagcaga agacttatta gatgtatatt ataatacatt atctactgct gatatggaat
    50221 ttccatattg gtataataga gaatatagaa aatcagatgg tgatatacca gtagttagaa
    50281 gagctaaagc tcttaaagct gctttttcac atatgacacc aaatataata cctggtgaga
    50341 aaatagtaat gcaaaagaca cgccattata gaggttcttt tccaatgcca tgggtaagtg
    50401 aaagcttctt tgttgctcaa ggagaacaaa tgagagaaga ggcaaagaaa ttagccagta
    50461 acacagctga tgaacttaca aaatttgggt ctggtggagg aaatgttact gaaagttttg
    50521 gaaatgtcgt ttcaatagct ggtaaattcg gaatgagaaa agaagaagta cctgtacttg
    50581 taaaaatggc aaaggaatgg gttggaaagt ctgttgaaga tttaggtttt cactatgaaa
    50641 aaatgatgcc agattatgat ttaaaagaaa acctaatgag tacacttata tgcatgtttg
    50701 attcagggta tacacttcct caaggtagag aagttataaa ttatttttat cctttaaatt
    50761 atggattaga tggaattata gaaatggcaa aagaatgtaa aaaggcagtt gcaggaaatg
    50821 catctggaga tgggcttatt ggtatggacc gtctatattt ttatgaggct gttattcaag
    50881 ttattgaagg attacaaact tggattttaa attatgcaaa acatgcaaaa tatttagaaa
    50941 gtatagagac agatttagaa gctaaaaaag aatactcaga tttagtggag atattagaac
    51001 atattgcaca taaacaacct cgtacattta gggaagctct tcagttgact tatacaattc
    51061 atatagcatc agtaaatgag gatgctatat ctggtatgtc tataggacgt tttggtcaaa
    51121 ttttatatcc ttggtatgaa caagatatag aaaaaggact aattacaaaa gaagaggtta
    51181 tcgaattatt agagctttat agaataaaaa tcacttgtat tgactgtttt gcttcagcgg
    51241 gcgtaaatgg aggagtacta tcaggcaata catttaatac tttatctata ggtggattaa
    51301 aagaagatgg ttctacagga gctaatgaac tagaagagtt attattagaa gcaagtatga
    51361 gatgtagaac tcctcaacct agtttaacta tgctatatga tgaaaagctt ccagaagatt
    51421 tcttaatgaa agctgctgaa tgtacaaagc ttggttctgg ttatccagca tgggtaaaca
    51481 actcaaatgg aacaacattt atgatgaaac aatttgctga tgaaggtatg actgttgaag
    51541 aagcaagagc atttgcatta ggtggatgtt tggaaacgtc tccagggtgt tggaaacagt
    51601 taactttaaa cggaaaaaca tactctattg caggtggagc aggtcaatct gctggctctg
    51661 gagttcactt tatagctaat ccaaaaattt tagaacttgt tcttatgaat ggtaaagact
    51721 atcgtatgaa tatacaagta tttgagcctc ataataagcc attagatact tatgaagaag
    51781 tcatagaagt atttaaagat tattataaac aagcaataaa tgttttagaa agagcaaata
    51841 acattgagtt agatatatgg agaaaatttg atacttctat aataaattca ctactaaagc
    51901 cagactgttt agacaaaggt caacatatag gaaatatggg atatcgttat aatgcaacat
    51961 taaacgtaga aacttgtggt actgttacta tggtcaattc atttgcagca cttaagaaac
    52021 ttgtatatga tgacaaagca tttacaatag aggaaatgaa ggatgccata ttaaataatt
    52081 ttggatttaa agatgcatta gaagtaggaa attattctat ggcagaccaa gtaaaagtag
    52141 ataagactgg taaatatgat gctatttata aggcctgtct tgatgcacct aaatatggaa
    52201 ataatgattt gtatgcagat aatattctta aaaactatga ggtatggtta tcaaaagtat
    52261 gtgaagaagc acaatcttta tatgctaaga agatgtaccc ttgccaaata tctgtttcta
    52321 cacatgggcc acagggagct gctaccttgg ctactccaga tggtagacta agtggaacaa
    52381 catattcaga tggttcagtc tctgcatatg caggaacaga taaaaatgga gtatatgcat
    52441 tatttgaatc tgcaactatt tgggaccaag cagtagttca aaattctcag atgaatttaa
    52501 aacttcatcc aactactata aaaggacaac aaggaactaa aaaattatta gatttaacaa
    52561 gaagttattt aagaaaaggt gggttccata ttcaatataa tgttgtagat tcagaaacat
    52621 taaaagatgc acaaaaaaat cctgacaatt atcgtcaatt aatggtacgt gttgctgggt
    52681 ttactcaata ttggtgtgaa ttaggtaaac caatacaaga tgaagtaatt gctagaacag
    52741 agtatgaagg ggtgtaaaat atgagaaagc atagtgattg tatgaatttt tgtgcagtag
    52801 atgcaaccaa aggaatttgt agattatcaa aacaaatgat taatttagat gatgcagcat
    52861 gtccagagat aaaagtaatg ccaaaatgta aaaattgtaa aaattttgtt gaagctaatg
    52921 atgaaggtat aggcaaatgt gttggcctag agaaagaaga ctgggtatat tcaacattga
    52981 atgcaattac ttgtgaagga catgtgttta atgagtagtc aaaagcaatt agaaggcatg
    53041 atttttgatg tacaaagctt ttcagttcat gatggtccag gttgtagaac aactgtattt
    53101 ttaaatggat gtccactgag ttgtaaatgg tgtgcaaatc cagaaagttg gactgtaaga
    53161 ccacatatga tgtttagtga attatcttgt caatatgaaa atggatgtac tgtatgtcat
    53221 ggtaagtgta aaaatggtgc attgagcttt aatcttgata ataagccagt tatagattgg
    53281 aacatctgta aggactgtga aagttttgaa tgcgtcaact catgttatta taatgcgttt
    53341 aaattatgtg caaaacctta cactgtagat gaacttgtgc aggtaattaa acgtgattct
    53401 aacaattgga gaagtaatgg aggagtaaca tttagtggag gtgagccact acttcagcat
    53461 gaatttttac atgaagtatt attaaagtgt catgaggtaa atatacacac tgccattgaa
    53521 acgagtgcat gtgtatcaaa tgaagttttt aataaaatat ttaacgatat tgattttgca
    53581 tttattgata taaagcatat ggatagagaa aaacacaaag aacaaacagg tgtgtataat
    53641 gatttaatat tagaaaatat atcaaacctt gcaaattctg attggaatgg aagattagta
    53701 cttagagtac cagttatctc tgggtttaat gatagtgttg aaaatataag tgatattata
    53761 tcttttatgc acaaaaataa tttggtagaa attaatctat taccatttca cagattagga
    53821 gaatcaaaat ggattcaatt aggcaaggaa tatgagtatt ctgataaggg tgatgttgat
    53881 gaaggtcatc ttgaagaatt acaagatata tttttggata atggtatagc ttgttatgtt
    53941 ggtcatgaga cagctttcta aaattaaata caagttttaa ttaaaaaaag gatgttacac
    54001 taaaagctag agttacatcc ttttttatgt ggtttttatg tagaaataat taagtaaagt
    54061 tttatatgtt ttatatattg tttatattgc tttatgtcca taaatattgc tttaaatata
    54121 ctttatatat tttttatagt ttaaattaca gttagagaat atattacgct tacttcattg
    54181 aagcagaagt tttgtggtaa aatactacta tgaaaaaatt taatagaata tattttagaa
    54241 acaactaaaa ataatagtta ggaggaagac atctatatga acaaatcatg gagcaacttg
    54301 attattaaga ataaagacaa aaaagtacgt tcagggtgga ttattatagc tgttatggct
    54361 accttttaca tacttcagta ttatttaagc atgatacttg tggaaataat aagaaaaatc
    54421 ctggttgcta caggtgacat taatttaaaa acaaactatt tttcagaata tgctaattgg
    54481 ttaaatgatg ttgggttaca gattgtattt caaattctaa cagattcact tatgattatt
    54541 attcctataa ttgtatggag atttattatg aaaaatccta ttagtgaaat gggattacat
    54601 tcttatagaa ttaagaaaaa agaaggatat gtaggaatgt tattaggaat atttaattgt
    54661 acagtaatct ttattataac aataacaatt ggtggtggtc atgttacttc atgggtacca
    54721 aaaatttcac ctttaacagt ttcttggata cttgtcttta ttttagttgc ttttgcagaa
    54781 gaaattttaa atcgtggttt ttttatggca gttttacgaa gatgtaaaaa tatttatttt
    54841 ataatgattg ttccatctgt tatttttggg ttgattcata tatggaatcc tgatgttact
    54901 tttttatctg taattaatat tataattatt gggattcttt tttcatatat gtttataaaa
    54961 tcgtcgaata tatggatgtg tataggttat cattttacat ggaatgtatt tcaggggatt
    55021 atatatggta tgcctgtaag tggacttcag gttccaggga ttataactac acaaatcact
    55081 catggtaatc tattaaatgg aggaatgttt ggtattgaag gtggaatttt gactactttt
    55141 gttaatctac ttagctttat atttgtatgg tattactata gagattctaa gtatgatttt
    55201 gttagtaata agtaagtgtg aataatgtta agaactaatt aattataata aattttaata
    55261 atatattcta ggcttaaagt attctgtagg attgttggaa atttacaaaa atataaaaat
    55321 aacttggaca ttctatttac tccaagttat ttttatattc ttttgtaaat agaaatggaa
    55381 cataaaaaat taaaacaacc atagatacta ttagtatatc atggtaagtg atagttgatt
    55441 ctttagaatt tgtgataaga aaagcactat tatttaaaac atgaattata gcacaaccag
    55501 atattcttag tttttatata aagatatcct aaaaatattg aaattccaac tatatgtatt
    55561 gaacgtaata ttattcctaa tattggcgct tcaggtgtat ataatgtaaa tattagtggt
    55621 aagtgccaca agctccaaca catacctaat attataactc ctaacttttt accaaattta
    55681 tcaaaaaaaa tattctgtaa aaaacctctc caggcatatt cttcactaaa aaaaatataa
    55741 atagtagtta atgatgttat tggagtaaat ataaatttta tgataccact ataattaggt
    55801 tgagcccctt gtagtattag tataaatgat gttggaacaa ttacagaaat cataaagtaa
    55861 ataattaata aagaacctag tttaatattt tttacaggat ataaattttt tttatttgat
    55921 gaatatatta taattgataa gctacttata aaagttaata ttaggtttat aattataaat
    55981 tcattattat ttattagatt taataactta agaacaatta ataatgaata aataagaaaa
    56041 aataatatga ttagtgaata aaattcatac atatcattat ttaatccttc agtataaaac
    56101 tttgcaatag caactgaaga tagaggtaat atcatcatga atgatgcaaa gttttctgga
    56161 tcaataaatt tattataata taacagtaaa cctaatgaat aagttaaacc aaagtttata
    56221 aagaaaaaga taataatttg tatttttttt ctttttattt catttttaat aaatactggg
    56281 tttagaatat ttatccatat taattttaag ttcattaatt taat
//
LOCUS       DAMJYC010000017        54190 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJYC010000017 DAMJYC010000000
VERSION     DAMJYC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.018"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(413..2800)
                     /gene="cadA"
                     /locus_tag="PC472_001093"
     CDS             complement(413..2800)
                     /gene="cadA"
                     /locus_tag="PC472_001093"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2726428.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2823..3188)
                     /locus_tag="PC472_001094"
     CDS             complement(2823..3188)
                     /locus_tag="PC472_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726429.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3387..4235
                     /locus_tag="PC472_001095"
     CDS             3387..4235
                     /locus_tag="PC472_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726430.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4672..5760)
                     /locus_tag="PC472_001096"
     CDS             complement(4672..5760)
                     /locus_tag="PC472_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726431.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5885..6613
                     /locus_tag="PC472_001097"
     CDS             5885..6613
                     /locus_tag="PC472_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2726432.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6815..7306)
                     /locus_tag="PC472_001098"
     CDS             complement(6815..7306)
                     /locus_tag="PC472_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726433.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7317..7760)
                     /locus_tag="PC472_001099"
     CDS             complement(7317..7760)
                     /locus_tag="PC472_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726434.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7750..8589)
                     /locus_tag="PC472_001100"
     CDS             complement(7750..8589)
                     /locus_tag="PC472_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726435.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8808..10193)
                     /locus_tag="PC472_001101"
     CDS             complement(8808..10193)
                     /locus_tag="PC472_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726436.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10366..11109)
                     /locus_tag="PC472_001102"
     CDS             complement(10366..11109)
                     /locus_tag="PC472_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2726437.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11129..12391)
                     /locus_tag="PC472_001103"
     CDS             complement(11129..12391)
                     /locus_tag="PC472_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2726438.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12575..13573)
                     /locus_tag="PC472_001104"
     CDS             complement(12575..13573)
                     /locus_tag="PC472_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2726439.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13557..15068)
                     /locus_tag="PC472_001105"
     CDS             complement(13557..15068)
                     /locus_tag="PC472_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2726440.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15122..16084)
                     /locus_tag="PC472_001106"
     CDS             complement(15122..16084)
                     /locus_tag="PC472_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2726441.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16132..17022)
                     /gene="rbsK"
                     /locus_tag="PC472_001107"
     CDS             complement(16132..17022)
                     /gene="rbsK"
                     /locus_tag="PC472_001107"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2726442.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17064..18077)
                     /locus_tag="PC472_001108"
     CDS             complement(17064..18077)
                     /locus_tag="PC472_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2726443.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18306..19283)
                     /gene="bioB"
                     /locus_tag="PC472_001109"
     CDS             complement(18306..19283)
                     /gene="bioB"
                     /locus_tag="PC472_001109"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2726444.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(19489..19752)
                     /locus_tag="PC472_001110"
     CDS             complement(19489..19752)
                     /locus_tag="PC472_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726445.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19822..20895)
                     /locus_tag="PC472_001111"
     CDS             complement(19822..20895)
                     /locus_tag="PC472_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2726446.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21143..21901)
                     /locus_tag="PC472_001112"
     CDS             complement(21143..21901)
                     /locus_tag="PC472_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726447.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21898..22815)
                     /locus_tag="PC472_001113"
     CDS             complement(21898..22815)
                     /locus_tag="PC472_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726448.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22815..23015)
                     /locus_tag="PC472_001114"
     CDS             complement(22815..23015)
                     /locus_tag="PC472_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2726449.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23020..24114)
                     /locus_tag="PC472_001115"
     CDS             complement(23020..24114)
                     /locus_tag="PC472_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726450.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24624..24764)
                     /locus_tag="PC472_001116"
     CDS             complement(24624..24764)
                     /locus_tag="PC472_001116"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2726451.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25074..26381)
                     /locus_tag="PC472_001117"
     CDS             complement(25074..26381)
                     /locus_tag="PC472_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2726452.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26446..26925)
                     /locus_tag="PC472_001118"
     CDS             complement(26446..26925)
                     /locus_tag="PC472_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2726453.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(26972..27835)
                     /locus_tag="PC472_001119"
     CDS             complement(26972..27835)
                     /locus_tag="PC472_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2726454.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27835..28590)
                     /locus_tag="PC472_001120"
     CDS             complement(27835..28590)
                     /locus_tag="PC472_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2726455.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28617..29105)
                     /locus_tag="PC472_001121"
     CDS             complement(28617..29105)
                     /locus_tag="PC472_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726456.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29123..29524)
                     /locus_tag="PC472_001122"
     CDS             complement(29123..29524)
                     /locus_tag="PC472_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2726457.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29527..30024)
                     /locus_tag="PC472_001123"
     CDS             complement(29527..30024)
                     /locus_tag="PC472_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726458.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30048..30452)
                     /locus_tag="PC472_001124"
     CDS             complement(30048..30452)
                     /locus_tag="PC472_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726459.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30682..33249)
                     /locus_tag="PC472_001125"
     CDS             complement(30682..33249)
                     /locus_tag="PC472_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726460.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33415..34158
                     /locus_tag="PC472_001126"
     CDS             33415..34158
                     /locus_tag="PC472_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2726461.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(35001..36218)
                     /locus_tag="PC472_001127"
     CDS             complement(35001..36218)
                     /locus_tag="PC472_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2726462.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(36300..36503)
                     /locus_tag="PC472_001128"
     CDS             complement(36300..36503)
                     /locus_tag="PC472_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2726463.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(36964..37194)
                     /locus_tag="PC472_001129"
     CDS             complement(36964..37194)
                     /locus_tag="PC472_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726464.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(37191..37613)
                     /locus_tag="PC472_001130"
     CDS             complement(37191..37613)
                     /locus_tag="PC472_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726465.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            38118..38471
                     /locus_tag="PC472_001131"
     CDS             38118..38471
                     /locus_tag="PC472_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726466.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(38531..38698)
                     /locus_tag="PC472_001132"
     CDS             complement(38531..38698)
                     /locus_tag="PC472_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2726467.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(38817..40736)
                     /gene="tet(M)"
                     /locus_tag="PC472_001133"
     CDS             complement(38817..40736)
                     /gene="tet(M)"
                     /locus_tag="PC472_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDF2726468.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(40752..40868)
                     /locus_tag="PC472_001134"
     CDS             complement(40752..40868)
                     /locus_tag="PC472_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDF2726469.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(41113..42045)
                     /locus_tag="PC472_001135"
     CDS             complement(41113..42045)
                     /locus_tag="PC472_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726470.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(42042..43043)
                     /locus_tag="PC472_001136"
     CDS             complement(42042..43043)
                     /locus_tag="PC472_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2726471.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(43040..45217)
                     /locus_tag="PC472_001137"
     CDS             complement(43040..45217)
                     /locus_tag="PC472_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2726472.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(45220..47667)
                     /locus_tag="PC472_001138"
     CDS             complement(45220..47667)
                     /locus_tag="PC472_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726473.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(47651..48157)
                     /locus_tag="PC472_001139"
     CDS             complement(47651..48157)
                     /locus_tag="PC472_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726474.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            complement(48132..48629)
                     /locus_tag="PC472_001140"
     CDS             complement(48132..48629)
                     /locus_tag="PC472_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2726475.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(48746..48967)
                     /locus_tag="PC472_001141"
     CDS             complement(48746..48967)
                     /locus_tag="PC472_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726476.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(49010..50215)
                     /locus_tag="PC472_001142"
     CDS             complement(49010..50215)
                     /locus_tag="PC472_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2726477.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(50238..50390)
                     /locus_tag="PC472_001143"
     CDS             complement(50238..50390)
                     /locus_tag="PC472_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2726478.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(50393..51778)
                     /locus_tag="PC472_001144"
     CDS             complement(50393..51778)
                     /locus_tag="PC472_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2726479.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(51807..52193)
                     /locus_tag="PC472_001145"
     CDS             complement(51807..52193)
                     /locus_tag="PC472_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2726480.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(52209..52523)
                     /locus_tag="PC472_001146"
     CDS             complement(52209..52523)
                     /locus_tag="PC472_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2726481.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            53035..53766
                     /locus_tag="PC472_001147"
     CDS             53035..53766
                     /locus_tag="PC472_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726482.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(53923..54129)
                     /locus_tag="PC472_001148"
     CDS             complement(53923..54129)
                     /locus_tag="PC472_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726483.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        16997 a        10251 c         6953 g        19989 t
ORIGIN      
        1 aaattatttt ttaaaaatat ttactttctt atattacata atttattgaa tggcagtcat
       61 actgttggtt tagagtattt taacacacac attttgttgt aaaaattaca actattaata
      121 taatatatat acaatcataa gtattataat ttttatgaat ctaatttaaa cttaatataa
      181 acttattaat aaaccttcta tgtctaaatt ttaaaattac aatataaaag ctctacacta
      241 aataaataca ttaaataaac ataatggata atgaatataa atttatatat gcaaagcgct
      301 ccaataaaaa ggtaccaata aagttaaaag aaacttattg atacctttca ttattacata
      361 cttcaatata taattataag ttttttaaat agtatggatt tacatttatc ccttatctca
      421 tagctctcat agcatttact actgctataa gtgccacacc tacatcagca aatatagctt
      481 cccacatagt agctactcct ccagcaccta gtatcataac tataattttt actcctagag
      541 caaatattat gttttgccac actatcttat tagtcttgtt tgctatttca attgctttag
      601 atatcttgct aggctcatct gtcataagca ctacatctgc agcttcaata gcagcatctg
      661 aacctaagcc tcccatagct ataccaacat cagcacgagc taatactgga gcatcattta
      721 ttccatcacc tacaaacgct attttctctt tttcagttct tccttcatat aaatcttcta
      781 atctatctac cttttcatta ggaagtaaat ttgagtaaac tgtatccaat tctaattctt
      841 gagcaatatt ttttgctact ttctcattat ctccagttaa cattacaact tcttttaccc
      901 ctatttcttt taagcttctt atggcatttt tactatcttc ttttacttca tcagagataa
      961 ctatataacc tctatatact ttatcaacag caatatagac tactgtgcct acttctttag
     1021 ctgatgaata agatatattt tcttttttca taagtttctc attacctgct aatataaaat
     1081 tgccattatg ctttactcta attccataag ctgctatttc ctcataactg tctattgtat
     1141 ctaaatcaat ttttttatta tagtaactta atatggactt tgctattgga tgatttgaat
     1201 ttacctccgc aatagctgca tattctaata attcttcttc tgatatacct tcaggattta
     1261 acttagttac attgaacaca cctttagtaa gtgttcctgt tttatcaaat actactgtat
     1321 ctacacttct taaagcttct aaatagttac ttcctttaat aaggatacca tttttagatg
     1381 caaacccaat tccactaaaa aaactaagtg gtattgataa aactaatgca caagggcaag
     1441 aaacgactaa gaatatcaat cctctatata accaatcact aaatacctca cctgagatta
     1501 caagtggtgg cacaaatgct attaataaag cagctattac tactattgga gtatagtatc
     1561 ttgaaaactt agatataaag ttttctgtct ttgatttttt aatgctagaa ttttcaacaa
     1621 ggtcaagaat cttagaaact gttgattctc caaaactctt tgtaacttca atactcaata
     1681 aagcattttt atttataaca cctgataaga tttcatctcc tttttcaact tctctcagca
     1741 ctgactctcc tgtgagtgca gaagtatcta acatagaaac tccatctact actacaccat
     1801 ctaatggtac tttttcacca ggttttacta ctattatatc acctacattt acctcttctg
     1861 gtgacaccac cttaacttca gaattaactt ttagatttgc atagtctggt cttatttgca
     1921 taagtgaagt tatagacttt ctggatttac caactgcaac accttgtaaa tactctccta
     1981 ctttatagaa aagcataacc cctactgctt ctgataactc ccctattgtt aatgctccta
     2041 ctgttgctag agccatcaaa aagttctcat caaatactct tccttttgat atatttctta
     2101 ttgctcttaa aagcacatca ccacctacta ttacataggc aactataaat actatattgc
     2161 taaactgact ttcataacct gtagctgttt gatatatacc aaatatatat acaagtgctc
     2221 caattataag tggaattaat tctttcttat ttgtatcatt tacagctcct gatttttcag
     2281 tagttctccc ttttactttg tcagtagacc ctacttgtat atcaagccct ggctctgttg
     2341 aatttattat ttcttttatt ttatttataa catcatcctc ttcaaagtta ttacttatat
     2401 ttactgtaag ttttttattt acaaagttta aattagaaga ttcaacttct tttaatttag
     2461 aaactttgtt attaatctct tctgcacaat gagcacagtt taatcctcct aatataagct
     2521 cttttttaat tgatgtttta ctagctgcat tttctttaga acttatttgt atatcaagac
     2581 ctggctctgt tgaatctatt atatctatta tcttttctat agtagtatcc tcattaaaac
     2641 tttcctttat atttactgta agttttttat ttataaaatt taaattggaa gatttaactt
     2701 cttgtagttt agaaacttta ttattaatct cctctgcaca gtgagcacaa tttaatcctc
     2761 ctagtataaa ttctttttta attgaattat ttgcttccat atatattcac tcctctcatt
     2821 tatcatttat aagtctctct aatatgattt aatccacaat caaatatctg acttatatga
     2881 ctatcatcta atgagtaata cactgttttt ccttctcttc taaactttac taatctagcc
     2941 tgctttaata ctctaagttg atgagatatt gcagaatgag tcatatttaa taaactcgct
     3001 atatcacata cacacatttc atttgcaaat aatgcatata atatctttac tctagtagta
     3061 tccccaaata ctttaaataa ttctgctaaa tcatataaca tttcttcatc tggcatagtt
     3121 gatttagcct ctgttactat ttcttcatgt actatattac aattacaatc atttatctct
     3181 tctctcattt ttaatcctcc attattttaa tatatgaata actgttcata tgttcattat
     3241 atgttatcat ttctaaattt gcaacaataa tttttattaa tttaatataa atagaatgca
     3301 catattataa acttttttaa tatatatatt attagaataa gtttttgtaa acttttttat
     3361 agttttctag gaggtgcaag tttaatatga gtaacaagaa aaaaaaggat ttagatacta
     3421 gttacatgcc ttacttagac ccagcgaact atgcagatta tattactaat gaagaaattc
     3481 cccaagatga attgagaaat gaccctcaaa tttctaatgg ttatacaaaa atacctaata
     3541 accagaacat aaataaccta tatcctaata atgtaaatca ttatcctaaa agtagtaatt
     3601 ctccaaacaa cttgcctaac acaaataata catccggaaa tatgaattac aatacatctg
     3661 ctacaaataa tatgcctaat aacatgaata ataatacggg tatgcctaat aatatgtcta
     3721 acaatatgaa tagtaatatg ggtatgccta acaatatgcc taataacatg aatagtaata
     3781 tgggtatgcc taacaatatg cctaataata tgaatagtaa catgggcatg cctaacaatg
     3841 tgcctaataa catgaatagt aatatgggta tgcctaacaa tatgtctaat aatatgaata
     3901 gtaatatggg catgcctaac aatatgtctg gcaatatgaa taacaatatg tatggtaata
     3961 caggtatgcc taataacatg aacagtaata tggggtataa tatgaacaat actatgggta
     4021 ttcctaactt ctcatgtaat caaaatatgc ctcctaatgt acttatgatg ataccaggag
     4081 ttatatgtca taatacaatg caaggtatgc ctgtagttat gcctagtact atgcctccta
     4141 atatatatcc tactccttat ggttctagta atatgtctgt acaaggtata ccacaagcta
     4201 ctaatattga ggaatttgat gaggaagaaa tgtaatatat atttttttaa gtaagttgta
     4261 taaaacatta ataattgacc ataataataa atgtattcct cataatattc ccccaatatt
     4321 attataggta tagaaagttg actgaatatg cgcttattcg gtcaacagat attaacataa
     4381 tctaataaga taaacataga aaaagtcaga taaaatttaa ttttatctga ctttttcatt
     4441 taaaaatgct ctcttgtgtt ggtgattcag aatagtatgg tactcgtatt tgagactaat
     4501 attggcaaac aacataaaga aaaatataaa ttttttataa taaactgtat aaaattttaa
     4561 taattgacca caataataaa tgtattcctc ataatattcc cccaatatta tagaaataaa
     4621 tttaagcgag attaaattca gttaatctcg cttaaataat gtctaaattt attattccaa
     4681 aaaatcatca aaatctaatg aacctgattc tgcaaaatca tataatgcat tttgctcaaa
     4741 gaaaaatttc tcttcaaatt taatatagtc gctataatta gattgactta tttcattgat
     4801 ataattgcaa tcattttcgt tataagtacc aataaaaaac ttatttccca ttaagtaata
     4861 tatagctttc atatcattat ttattatata tacattttct tttacatggt cattattaaa
     4921 attaaatcta gtaaagaata taccttcatt catattactt tttaacagaa atggattttc
     4981 tctataaaat actcctgcaa attcttcagg tgaatttaca ctgtaaacac ttacaaattc
     5041 acttttagaa atctcatcaa atacctcaaa atcatacata ggttctttat ctgtcactaa
     5101 tagagaatta attctaaaac cagtttcata acacttactt atgcaaaaag caattttaca
     5161 tatataatat ccatcattat cttcatataa agcttcactt atatatctgc cttgaccttt
     5221 atcacttacc acgtttttaa gtaatgttcc atttatatta gtaatgtgca tatttgcaat
     5281 ctcgtcactt ccagaaaagt actttaaact ttctctatcc catgctataa atctcatggt
     5341 catatagttt ataaattcaa cttcctcgtc aactctttta catattttat tgtataattt
     5401 ttctatagtt aaatctgttt tatagttttt tataatatca atatactcac atttatctcc
     5461 tggcaatagc ttatcataat aaagattctt attaccaaaa taagatacta aaaataaaga
     5521 ttcatccttc gttaattcaa ctctatctga accaaatcct cccatcaatc tttcttcttc
     5581 catataagct tcttcttgtg ttgggttatt taaactcaca taatccgcta atcctaatcc
     5641 ctctgcatct aataaaaaat attgacaaaa gtgattgttt tcatcatcta tccaatttat
     5701 tataagacct aaactgccca taagtctaga atttgtagcc tttgcaaaat taaaattcat
     5761 atagtcacat cctttaaact ctaaaatact tgttttttaa tatataaatt attatacata
     5821 tattttaaca acttagatac aatttataat ataatataag gaaatgatta attgaggtgg
     5881 tcttatgaaa aatatagcag ctttttttga tatagatggt actctttata gagactctct
     5941 tatggttgaa cattttaaaa aattaattaa atacgatata attgaccaaa aaaattggta
     6001 taaacatgct agagacactt ttatggattg ggataaaaga caaggtaatt atgatgatta
     6061 tctattagaa atatgtgacc tttatgttga ttcactaaaa ggattagata aaacctgtat
     6121 caactttaca agtgaccaag taattaaatt aaaagcagat agagtttata aatacacacg
     6181 ttcaaggatt gagtggcatc taaatcaagg acacatagtt atatttatat ctggtagccc
     6241 tggatttctt gttgaaaaaa tggcaaaaaa atataatgta accgattttc taggaagtga
     6301 ttatgtcttt gaaaacaata tatttacagg cacagttatt ccaatgtggg attctataag
     6361 taagaataat gctataaacg attttgttgt aaagtatgat ttagatttat caaactcgta
     6421 tgcctatgga gatactaatg gtgatataaa tatgctaaaa agagttggaa atccaatagc
     6481 aataaatcca acaaaggaac ttttatctca aatatcactt gatgaggaaa tttctaaaaa
     6541 tgttgagata atagtagaaa gaaaagatat tatttattct ctatctacag atgtaaatat
     6601 agtggatatt taatttatac tatctatttt aaaaacttat attatatcta atatttatat
     6661 ctaacatata aaagctttga ctaaaaatca aagcttttat atgttgcact aagaatatat
     6721 taaatttagc tttatgcttt aatgtatatt catatatatc atttacactt atctaaacta
     6781 caatttcatt aaaattaaat ataaaaatca actattattt tttaaatagc atctttctta
     6841 atctattatt gtaataacct ataaaagaac tgtaaacata gtcatatact ataaaggcta
     6901 tctcaaatga acctataata aatatgttta ttggaatata aactattgtt cttaataaaa
     6961 aatacaatat aagtatcatt atatttgcaa atactaattt cataaaatac tcaatatata
     7021 tagacctatc tttttctata agatatttta caataccata tattccaaag gtaagtgaat
     7081 atacaatcca ttgagactta cttgccatca ctataaatcc taaaactata gaacctatat
     7141 aaaaagcaat tccactctta aagccccatt ccattattac aattgatact aaaagtgatg
     7201 ctaaccccat aataaacaat gtatttacag gaataatgtt tgtaagagtc aacaatatca
     7261 catttaatgc taataaaact cctccataag caacttttat actcatattt atcatcctaa
     7321 caacaagtta ttaaatctcc accaaaacat tcacaacatg aatctaaaca ccaaagttta
     7381 gcacaatttt cacaacaatc gtcacctaga caacaacagc aatcatcaca gcaacagcaa
     7441 caacatccat ctaaatcatt acgtcttctt ctattataat tgtcagccct attgctataa
     7501 tcatttctat attgtgtata acttctaagt gcatcactat attcactatt ttcaggctcc
     7561 ataaattttg ctctttttaa gaagtcttca ccttcttcat aatatcctat gttcattgca
     7621 gaaaaaccag ttaaataata ccactctgca catttgtcag tcaaagtttt taaaaaatca
     7681 tatgcttttt taaattcact attttctatg aatgatcttg ctctcataaa attttcatta
     7741 ttataagtat tactgtacat tctttccacc tctactttct aatatattaa tatatcttaa
     7801 ataaacacca gaataaatta tattttctat tattccaaca tttttattta gatttagatt
     7861 atcaattccc ctagctaaaa atcctaatga tgttactatt atttttttta ctttttcttt
     7921 taactcatca tttttatcaa tatactctat aaatggatta tatcttcctt tcttaaaatc
     7981 ttcatctaaa tcttcatagg catctagtag atatatatac ttacctatat taaaacctat
     8041 cattcttaag tttctctcat tttcatcttt tttatagaca aatacttcac ccattatatt
     8101 tccaaatata ctagatactt catctatatt tatattttta tctttttcta acttatttaa
     8161 ttcatttagt tgttgtttta tatactctgc tttttcagga tacttttcat atgctaattt
     8221 taatttgttt ttatatatat aatatgccaa tttatctttt atgcgtttat catccattaa
     8281 attatcttcc aatttataat acgttaaaag aatattcata cttgctgcat attctgtaat
     8341 atcatttgta attacctttt tctttttaaa tggatttact atacacactt cctctgtact
     8401 tatagactct gggttatata ctgctgttaa taaaacaatt aaaaatgtta tatcatagtt
     8461 caaagaaaac ctagagattt ctccatgatt tctttttaag tatttacata gtccacaata
     8521 gtaagctttg taataatcat attctctaaa tgttaaatcc attttgttta ttttaacata
     8581 tccaaacata aatactcctt tttgtactat tttttattta gattatatat agtataacac
     8641 tttttatcaa gattatataa tttacagtat tctaagaaac ttaaaaaatt ccttctaatc
     8701 attgtatctt aaatttaaaa taattagaag gttgggttaa aaataatatt aaattatata
     8761 cttatattaa ctcatacaag ttagttatgc ttatatcaat ttaagtttta aataattggt
     8821 atatatttaa aactacctac agtatccttc aattcttttt ttgctttgtt tataaccaat
     8881 tcatcaagca ttacatcatt tattgaacat gatatagatt tagatgccaa taacattgcc
     8941 ttaaatccca tatcagaacc tgcactggca caagattgcc atgtatgggc agaaatacca
     9001 ataggccaaa ctgaacaaga acactgtgct gtaggaatta tgtagctaac atctccaata
     9061 tctaatgata atgatataca gttattatga gtaaatgtat catccgtaac ttcatcatgc
     9121 agattcatct tagtaatatc cttatttcca cttaatacac tagaaacact acttctttta
     9181 tctatagaca ctgtctcagc taatttatct gcaaaatagt agtcatcttc attatagttt
     9241 tgcacaccta ctaatttcat attttgtgat agaatatctg ttaatattgt atttggaata
     9301 tagtcatata ctccacttat aatgtcatat tccataatag taccagtcat taatgctgct
     9361 ccttttgcta tatcaacaat cctaccaaat acatctctta catgctccat tttaactcct
     9421 cttatgtaat accacacttc tgcaaaatca ggaactatat ttggcatttt tccaccatta
     9481 gtagttacat agtgtactct acaattatca aatatatgtt ctctcaaata attacaacct
     9541 atattcataa gttctactgc atcaagtgca cttcttccgt tataaggagc ttgtgctgca
     9601 tgggcactaa tcccattata tctaaatttc acagagtaat tagcaagtgt tggtattctt
     9661 attggagtat ttatatcaaa tggatgccaa ctaaaagcac aatcaattcc atcaaaaaaa
     9721 ccttttttta tcatcaatac ttttccagat aaatcttctt ctgctggaca tccatagtat
     9781 tttatagtcc ctttcagctt ttttagtttg attaattcct ttactgcaat tacagctcct
     9841 attgaacctg taccaagtaa gttatgtcca catccatgac ctgcaccacc atctacaata
     9901 ggttctttag tagtggatac tttttgagac aaaccatcta aagcatcata ttctcccaaa
     9961 actgctataa ctggtctacc ttctccataa ctagccacaa atgctgtatc cataccttct
    10021 aatccctcta tactaaaacc atgttttttt aaacattctt tttgtaaatt tgaagcatag
    10081 tattctttat actgaagttc tggatttttc catatcttat ctgatatatc tcttaattct
    10141 ccctgtatct catctatata ttccatcaaa aactttttat ttaaattatc catattaatt
    10201 atataaacct ccctgtttct aataacttat cgattatcag ccaaaatgac taattttaac
    10261 tgatttaaaa atattttcta ccatacaacc attttataca ataaaacctc ttgacttgta
    10321 agtttcattt tatcaagtaa gaggttttca agttttataa aatttttagc atgttaacca
    10381 atcaagcata ttaacccata atttattata gtatttccaa tcagtaaatt cttttggacc
    10441 ccagtgaggt gcacagtcag aactaaagat agcagatttt ccttttccaa actcgccaac
    10501 agctacaaat gggtctcctc caatagttgc taaaacagga caattatctc tagctactgt
    10561 tttgttatat cctaaaaact ttggccattc tgttggtata ccttttaata ctggatgttc
    10621 agagtcaata acttctggaa ttattcctgc tggtttttca actctatcat ctttatctat
    10681 cattgttatt ggtaatacat ctttgatagc agtttctcca aatctagctt ttgcatctat
    10741 tcctgtaaat gacatatatc cacctaccat gattaacgcc ccaccattat ttacatactc
    10801 tttgattagt tcaagtctat ctggattact attgcaatct ataaaagtac tattagataa
    10861 taagaacgta ttagaaccta tatcagataa tattatacag tcatactcat ttaactcttc
    10921 taatttatac ggaaaactat ctgctgctac atgtgcaggc atatgaactg tctcatatcc
    10981 tccagattct attgcttctt tcaaccactt tacagcttct tcataatgag ttgtaacaaa
    11041 cgtatcaaac ccttttatat gagttatatt ttttacccaa gattccccta ctattaatac
    11101 ttttttcatt tatatattcc tcctactttt ataagtttaa tattagactt gcatacataa
    11161 gtatactatc taaatactcc ttaatttcaa catattcatc aattgaatga cattgttcta
    11221 ttgaacctgg ccctaatata agtgttggta tttcagctaa atttttcatt actctcgcat
    11281 cattgccagc tgttgaacct tttatctcta atttttttcc tagagtttct tctagtattt
    11341 tatgtgcatt cactacaaaa tcagaatcct tgtccatctc aaaacctaac cctgattgat
    11401 atatgtttat atttggttta ttatctttaa gccataaatc tccattagcc cttgtcatta
    11461 tgacatttgt tacatcattt acaacttgtt cataactcat tatatttggc aagaagtgta
    11521 cacataaatt aaatacacac ttatctggca cagtagaacc tgctgttcca ccatttatta
    11581 cacctaaatt tattgtaggt gaaggtaata gtggatgttt atatatcata agccagttat
    11641 gttccaactc ttttatatct tgaagcaaca acatagcctt ctcaattgca tttacaccct
    11701 cccacttact accacagtga agagacacac ctttaacctc aacttcaaag aatacaaatc
    11761 ccatatgtgc aactattaag ttttgttctg atggttcaca aattatgcaa caatctccat
    11821 caataccatt cataactgca tttatggtac catttccgcc accttcttca tctactactg
    11881 acattatttt tacattacca ggtacattta gaccactatc ttttataagc tttacagcta
    11941 atattgatgc tataagtcca gatttcatat cagcagttcc aagaccataa agttttccat
    12001 tatcttctgt agcacgatat ggattatatt tccacttact tatatcaccc ggtggcatag
    12061 tgtctacatg accattaaat acaattgtct taccaggtaa atcatcacta tacttacaaa
    12121 ttaaattata tctatcttga taattgtgac ctagatttcc ttctttatat atattttttg
    12181 cttttttaat taattcttcg ctcatttctt gtctatccac actaaaacct atgtaatttg
    12241 caagtttttc aatatattcc tgtccttctt tttcaaatcc acctagtatt ccatgaccaa
    12301 catcttcagt ctttatactt acaagttctt ttaaataatc tatgtattct tgttttttac
    12361 tatttaatgt atctaagaga atttttttca tatttcctcc taagatttat ttttacattt
    12421 aaaatttcaa gtctaatctc atctatttaa gccatagtaa actttttcac ttagcatatt
    12481 ctcattcaat gtataaagat gtatgcaaat taaagtataa aaatatatac aaaaacttaa
    12541 taatttttat agtttataag ctaaaattta attactattt agatttagct gattttttta
    12601 attttatatt atttatccat atagcaaata acattaccaa accttttata atcatttgat
    12661 tatatgaaga tacacttaat aagtttaatc catttcctaa tattcctatt attccgacac
    12721 ctatacatgt acctactata gaaccagaac cacccatcat attagtacca ccaagcacaa
    12781 cggctgctat agcatccaat tctgctccat ctccaacagt tggtgaacct gatgccaaac
    12841 gtgatgatag tattatccca gctaatgctg ttagtagtcc agctagtgcg tatactttta
    12901 ctataacttt atttactttt actccacata atctagcggc ctcttcattt ccacctactg
    12961 catgtacatg acgtccaaaa ttagttcttt ttaatagaaa atgtcctgct attacaacta
    13021 taatcataat tataactggt attggtattg gtccaatata tcctctaccg agcatcttaa
    13081 atgcatctgt atttactgat ataggtcttc ctcctgtaaa tacaaagctt gccccactta
    13141 ttatcgttaa catggctaaa gttacaatga atggaggtag tttttgtgtt gaaataattg
    13201 caccttttat gactcctaat attgtcccta ttgctaatgc aagtatcata cctacccata
    13261 taggtgcatt ataattcact actgttgtag cccaaaatgt acctactaaa gcaattactg
    13321 ctcctacact taaatctatt cctcctgtaa ttattacaaa tgtcatacct actgcaagta
    13381 ttgcatttat tgatatttgt cttccaacat taattaaatt atctaaagtt aaaaagttag
    13441 atgaagctac actcattatt ataaaaatta ttacaagtcc tacagctgca ctcccttggt
    13501 ctctaaatac acttgttaat tttgtcaata atggagaaga tttttcttct ttatgcttag
    13561 ttattgaact cattaatttt acctccgaaa gccaatttta atacattatt ttcaaccatt
    13621 tcttcacctt ctaaaaatcc acttatatga ccttctctca taaccattat tctgtcactc
    13681 attgatatta attctggtat ttcagaagat atcattatta tacttacacc actctccact
    13741 aaatctccca ttagattgta tatctctgcc ttagcattta catctatacc tctggttggt
    13801 tcatccataa tcaatatatc tggttgtaat aaaagccatc ttgctattat agctttttgt
    13861 tggtttccac cacttaaact ttgtataact tgattcatat gtggtgtttt tatctttaaa
    13921 gcttctatgt atttttctaa aatctctttt tccttttttc tatctataat tgatttaaac
    13981 ttagatatct tttttaaccc agccatagtt gtattttctt tgacactcat ttgtaaaaac
    14041 aatccagtac cttttctatc ttctgttatt aatcctagtc cattttctat tgcatctgat
    14101 ggagttttaa actttacttc ttcattgttt aaatatattt ttccagaatc atatgttcta
    14161 agtccaaata tagcttctac tatttcactt ctcttacttc caactagtcc acctattccc
    14221 aatatttcac cttttcttat attaaagcta atatcattaa aaacattgtt cttagttaga
    14281 ttttccactc gcataatttc ttcttttatt tctacttttc tttttgggaa aaattggtct
    14341 agctttctat ctatcattaa attaactaat tcttcctcag acgatacatc tttggtattt
    14401 aaagtcgaaa tcgttttccc atctcgcatt atagttattc tgtcacaaat ctcaaagagt
    14461 tcttccatcc tatgggatat atatattata ctaactccac tctcttttaa atcatttata
    14521 agtctaaaaa gtatttcagt ctctttttta cttattgaag aagtaggttc atccattatt
    14581 aatactttag tttctgatga taaaactctt gctatttcta ccatttgttt ttgagctata
    14641 ctaagctctt tgactaaact atttggattt atatctattc ccatattatc gaaaatcttt
    14701 ttagcctctt cattcatttt tttgtcattg gttaagactt tgccatttat ttcttttccc
    14761 ataaaaatat tttgtgctac tgttaattcg tcaaaaactg ttagttcttg atatatggca
    14821 tttataccta tctttttaga gatatctggg gatatattct ctatttcttt gccttcaaaa
    14881 ataaattttc cagaagtttt cgaatgtgca ccagttaaaa tctttatcat tgttgattta
    14941 ccagcaccat tttcacccag tagtgcatga acttcgccat gaaataactc aaaattaaca
    15001 ttatctaatg ctctgacacc aggaaattct ttagcaatat tacttaactt cagaattata
    15061 ttactcataa tgacactcct ttttaaatag gatttctttc aaataatctc attttatttt
    15121 actaattctt aaagtctttt atattttcct tatcataaag taaagctggt actggtatat
    15181 tcttttctac cttttcccct tttatagctt ttactactgc ttctacagct aaacgtccca
    15241 tctctttagg ttgttgttga atcatagcaa gaatagttcc atctgttatt ccacttgatg
    15301 cttcttcagt taagtcaaat cctacaatct taacatcctt attttttgca gattcaagtg
    15361 cagctttagc tcctagtaca ctattttcag ctgttgcata tattatatct acatctggat
    15421 tagattgaat taagttttct actgtattaa gagattcttc tctgtcatat cctggttgtg
    15481 aatttaatat cttaacattt gctgaacctt ttaatgcatc tttaaatcca tctattctct
    15541 gcatttgtat ttgagatttt aaatctgtca ctattgctat ttctgacttt ccacctaact
    15601 tttcatctat gtatttttta gtgtattccc ctaatttttt accagcatca taactaactg
    15661 ttcctacata acttgtaata ttttcagtat ttaatacagc atctactgta actactggca
    15721 ttttttcaaa tgctccttct actgctggta ctatagccga attatcagtt ccaagtacta
    15781 tcattccatc tacattttgt tgtataaaat cttctattgc tgatgtttgc ttagctacat
    15841 cttggtctgg gtcatttact ataactttca cacctaattc tttagctttt tcttttattc
    15901 cctcatcaat atctataaaa aattgatgtt gaagagttat tgtagatact ccaatagtta
    15961 aatctttctt gtctgaacca cctttattat ctgatttact tggtccttcc attgaacacc
    16021 ctgttaacat tcctattgct agtattccag caattcctaa agataaaatt tttctcactc
    16081 tcataattaa cctccatttt taatagtttt aaatttgaac aaattagatt tttatttatt
    16141 atttaaatac ttttcaacat cttctttata gggcatacca gattgtgcac ctagtttagt
    16201 tacagaaaga cctgatacta ctactgcata ttctactgct gtatataaat ttttcccttc
    16261 tgttatagcc actgccaatg ctccattaaa tgtatctcct gctcctgtgg tatcttgaac
    16321 atcaacactt atacaagata catgcttgat ttcctcacca tcataaaatc tagctccatt
    16381 ttttccttct gttattacaa gtttatttgg atattttttt aatacttctt ctatcccttc
    16441 atttgtgtca aaaactatct tatattcatg ttcatttggt gttaagtaag ttactttttc
    16501 tattatatct tcatttagtt ttactgctgg tgctggattt aataaaaccc taatcctatt
    16561 ttcaaaacag aaattcacta cataatttat tgtttttaat ggaatctcta attgtagcaa
    16621 tactatatct gcattcttta tctcttcttc attcttttta attaattcta tatctactag
    16681 ctcattagtc cctggtacaa caacaatact attatcattt tcacataatg ttataacagc
    16741 aacaccagaa cttttgtgat tatttctgga tactaagcta taatcaactt tattcgttct
    16801 aaaatttctt attaactcct ctcctaagcc atcttcccct atgcatgata tcatttttac
    16861 atttgctcct agtttagaag ctgagatagc ttgattagcc ccttttcctc caggtgatgt
    16921 aaaaaaacta ttacctaata ctgtttctcc tttttctggt cttttatcta cagaacatac
    16981 catgtccatg tttacacttc ctatgacaac tatgtttccc attacattac tcctttacat
    17041 atctatttat gtctctcgtg ctgttattca ctttcttttg ttgaatctct tatgaccaat
    17101 tctggtttga aaatcaattc atcaaacgat tcttttttat ctcctcttaa acaatttatc
    17161 aatatttcac tggctttata tcctatatca taagacggct gttttactgt ggtaagagaa
    17221 gggcttatta gacttgatgt ttgaatatca tcaaatccca cgacacttat atcatttggt
    17281 atacttaatc ctctttcttt aagacctctt atgacaccta ttgcaatcaa atcatttgca
    17341 caaaatattg catcaaaatc tatcttttct aaatttttta taaattcttg tccccattca
    17401 atcttatatt ttccttcaaa tacataagca ggattgtagc ttaaattgca ctcgattaga
    17461 gcctttttat atccttctaa tctatctact gctatttcat tttgaagtgg accacttagg
    17521 tatattattt tcttatttcc aatatctaat aaatgttttg tagctatata ggccccttca
    17581 aagttattta ttttaacaat tccttttaag gtttctttgt tcatacttat cttcttatca
    17641 atcaagacag ttggtatttt tatatcttta taatttgctt ctttccagtt tgtatttgaa
    17701 gatgctgcaa atataattcc atctatcatc ctctctgata gtgttcttat tccttcatat
    17761 tcttttttag catcttcatc tgtattacat aaaattatat tgtagccttc ttcattagct
    17821 ttatcttcta cacctcttga tacatctgta aagaatggat ttcgtatatc tggtataaga
    17881 agccctatag tattagtttt tttagttact aaactttgtg ctaatttatt tggtacataa
    17941 tttttttcct tcattatttt caaaattttt tctcttgttg cctgacttat attttcatct
    18001 ttattattaa tgacttttga cacagttgtt ttagaaacac cagccaattc gccaatttct
    18061 tttatagtta tactcaagaa gttcaccccc aaaatttagg aaaccgtttt acttaaccgg
    18121 ttacctagat actatcacat ctttttttca tttgcaacca ttttatataa attttgatat
    18181 ttttcccttt tttcaaaata ttgcaactta taattcaatt ttaatattta aattaatctg
    18241 caaaaaaaaa ttaatatact tttataatta tctaaaagta tattaattaa tttaagcagt
    18301 atttattaaa attcaaatcc ttgctctata atcatttttt tatcattttc aatcccactt
    18361 ccaacagttg tcaaataatc accaacaatt gcagaattta ctcccccatt aaatccaact
    18421 ttgtcatatc cctttaatga tagccttcca ccagcatacc ttatgtacac attaggaatt
    18481 ataaacctaa atactgctgt tgtttttagt atttccattg gttctaatac atcataattt
    18541 tccattggtg tattcttaat aggatttaaa atgtttattg gaaatgattt tacatttaat
    18601 tctcgtattt caaaagccat ttttagtctc tgttcttcac tctcattcat tccaattata
    18661 ccgcctacac atacatctag cccagctttt ttagcatctt ttatagtttt tatcctatct
    18721 ttatatgtgt gtgttgtaca tatattatga aaattatctc tacacgtctc aacattgtgg
    18781 tgatatgtag tcacacctgc tgacttcaac attttagctt gctcataatc tataatacct
    18841 aaagatgcac ataattttaa attagtattt tccctcaatc cttcatagat atttaaaata
    18901 ttattaaact cctttcctga cataccttta ccacttgtaa ccaatgagaa tctatgcact
    18961 cctttactct ccatctcttt agctctattt aagatttcat tgtaatctaa taatgaatat
    19021 tcttctacac cagttttata ataagaagac tgagcacaaa atttacagtc ttcactacat
    19081 ttgccagatt tagcattcat tatcgtacat aaatctgctt ttcttcccat aaaatattct
    19141 cttatttcat ttgcagattt taatagaata tcaaaatcgt ttttattatt tgtatcaagg
    19201 cttattaaat taagagcttc ttcataactt atttcttttt ctctcaatac tttatttttt
    19261 aattttatta tgtatttttc catatatcct cctatttgtc aacttaatat ttttatgtag
    19321 tttacttatt atatatagta ttgcatatat aacatttatt ggtcaacttt attttagaca
    19381 caagtttact atacacaaaa aaatactgct tcaaaacaat attagtatat agattataca
    19441 tattattatt aaagcagtat tttttattag aatataattc ttactggttt ataaaactat
    19501 ccttgtctta actccatcca agctttccat tgtaataact tctaagcctt catattctct
    19561 aaatagccat tttaaatcct tcatggcttg attaacctga ctttttgtta gattatttat
    19621 aaaatattct tttctagttt cctcatactc tttgctagtc aaaaagtttc ttagaaaatt
    19681 aaacgcaaat acacttggaa tattgtttac attagccttc ttgctgtttt ccttctcata
    19741 gtaaattttc ataaatgcat actcctttga tacttttaga tttctcttac aataattata
    19801 tgcatttaag tgctactatt cttataattt tattaattca tattctttac ttcccatacc
    19861 catttcacat gcatagtcta ttattctagt tccatttaca tgaggatgtt catgtttgaa
    19921 aacatcatga cccatttcct tgcaaatcaa atcatagcat gcttgttcaa tagctacagg
    19981 gtctgttgat gctaatattc ctatatcatg ggcaattggt ctacctgacc aaggtacaca
    20041 atcacaaagt ggagttacat tcataacaaa agttatatac cctaccttgt cttttttatt
    20101 tgatactgct ccataagcgt attctgccat cttttcgaca aacacatctg agtcactttc
    20161 ccattgtata gttactgctc tagttggaca gacagttgga cattctccac atccataaca
    20221 tacatcagaa tctataactg cttttttatc cactatactt atagcttttg taggacaaga
    20281 attaacacac tttccacaac ctacacactt cttttccttt actactggag taacatcaga
    20341 atgttgcatt tgttttcctg ctgcacttgc acatcccata gctaagtttt ttaatgctcc
    20401 tccaaaacct gctgcttcat gacctttaaa atgactcatt actatcattg cagaagaatt
    20461 ataaatttct ccacctattt ttacactttc aaaatgtttt ttgtctattt ttacattctc
    20521 ataatttcta ctatataatc catctgcaat tattactggt gcatttacaa ctgcataagc
    20581 aaatccattt tctatagcag ttgtaagatg gtctacagaa tttgttctac tccctgtata
    20641 cagtgtattt gtatctgtta aaaatggctt tccttcgcag tctttaacct tatcaaccac
    20701 ttgtctaact gccacaggac tcatatatgt agtatttccc ttttctccaa agtgaagttt
    20761 aattgcgact tggtcatttt tttctattaa atctttgaat cctgcttcat caaataatct
    20821 tcttacattg tttggtacgt ttttattttg cgaatttgag tataaatcac aaaaatatac
    20881 ttttgacttt tccatatcta caccccttta aaatatttac ttatactaat tttatcactt
    20941 ttaaagtaat ttttagtata ttttaacata attaaatatt cttatattta tttgatacaa
    21001 ataatattat ttgtattatt ttctacactt aattctatat attaaaaatc aaaaagactt
    21061 gaaacttaag tccaagtctt tttgattttt aggtgtagtt tttaacaaca taagattact
    21121 ttaccttgtg ataaaggtat tattaaactg gttttttatt aaataaaata acagctgcta
    21181 gtaaacatac tacagaaata agaactgtta taataattgg aataataaat tctggcattg
    21241 ccactttccc tgaaatcaag tctatatttt ttgacatcaa gaaattagga gtataaggtg
    21301 caatctgttt aggaatagaa attaaactca ataaaagagt tgccaccaat acaagtaaaa
    21361 cactggcaaa tgctggtcta aatagtacac acccaagaat taatacacta atatacataa
    21421 ttcctataag ccataaatta aatgctgaaa atataatatg attaagattt gcagttggcc
    21481 aaaaatacat tgtatatcca taagcaaata aaaaactcaa ccaaaaacca attgtcataa
    21541 taatcaccgt aacagagaat ttagacaata ctatagatga acgtgataaa cctttagtca
    21601 gcataataac aagtgtccct ttagaatatt catttgataa aatattacaa aaaataatta
    21661 gagttaaact cataccaaga ccagagatat tgctaaaaaa ttgtgtccaa gaatctaaag
    21721 cagttggtgc agcagtaact tttaaagtcg gtgcaaaatg agcaatcaag tctggcataa
    21781 attttgcaga aataggacta cttataccaa atattagaaa taaaataatt aaactaaata
    21841 atttataatt tcttaaattc tctttaaatt ctttttttgt aaatgctatg tattctctca
    21901 ctttactacc tccaaaaata aattttcaag tgataattct attagttcca tctttactgg
    21961 acaaagattc atttctgcga gtgtagatat aactgttttt tgtattgctt ttatatcttt
    22021 accatgcaaa acaatttcca tattagtttc ttctgaatct tttagtaagg atttaatacc
    22081 atcactagac ttaaactttt gaatttcatc attacttgca aactccagta aaagtctatc
    22141 ctttccgtgc atacttttta tttctgaaag tgtaccacta agggcaatgc ttcctttgtt
    22201 taatatagca acatggtcac agattctttc aacatctgat agtatatgtg ttgaaaatat
    22261 tacagttgtt gagtctttga ttttaagcat aatatctaat atttcttttc gaccaacagg
    22321 gtcaagtgca cttgttggtt catcacaaat aagtaatttg gggcgtgaaa gcaaagcttg
    22381 agctatacca agtcgttgct tcattccacg agaaaaccca cctattcgtc tcttttcatt
    22441 tctaagaccc acaagtgata acaactcttc actccttatc tgtatttctt tttttgaaag
    22501 tcccgtaatt tcaccacaaa gagaaagata ttcaagtggt ctcatataat tgtaaaactc
    22561 tggaacatca ggcagataac ctacatgacg attagaactt gttttcccat aggtcacttt
    22621 ctcaccacaa acatctatag ttccactatc tggtttaagt aacccaagta ccattttcat
    22681 tgttgtagtc ttacctgcac cattcttacc tacaaaccca aagactgacc cttctggtac
    22741 agtaaaattt aaattatcta ttatctttcg attaccaaaa ccttttgata atccatttat
    22801 tgagacaata ttcatcattc ttcacctctt cctattgtaa aatacaaaat tggtcctatt
    22861 aaactaataa acagtactac aataatccat attgccttat ttccaatttt ataatttgga
    22921 tgtctcaaca catgaacaag tgcagtaatt ataagtatta aatctaaaat aataacagga
    22981 accaaaagtg gtaagtattc catcaaattt tccataatct tattcctcca ataaagataa
    23041 tttttctata agaaatttgt attctttgtt ttcacttaat acagaaaaca ttggattatt
    23101 tttaatagca gcaacaatac ctgcttttac gattgtatca tcacgtgtaa tacctgttcc
    23161 aatttcagaa atccatctgt caagtttatt aaaaaagtca tccccatgta ttataattgg
    23221 gtatatatca cgtgttgcta aatctacata ttttcgtaat gatttaatag ctccttcatt
    23281 atcaccatgt attaaattaa tttgtgcctg tgttaaatat gaagaaagca taatagcagg
    23341 agataatgta tcaagctcaa atatatctga aattgctaga atacgtcttt ctatttcctt
    23401 catttgtaat ttatctgcat tttgcaaaat acccactagg ttttggacta atgatataag
    23461 gttctgatat gcaccaagtt gaaatgtttc tcttgcttca tccatttggc cattcatcat
    23521 ttgcccctgt gcaagaagta tagattcatt tatcatggga aaattgctgt tttgcaataa
    23581 atcaatcact tgtatgggtt gatttaacaa tatataacaa gttgcctcca tactttttgc
    23641 ttgacggcaa atatctatgt cattacaagt ttcctgtata cgaataaata tttctaatgc
    23701 ttcactaatt aatagttcac gctttttttc atctacaata tcatagtgat taactattaa
    23761 aagacccatc cgaaaaatca aaggaaaaca tgaataatat ttttttatga tttctcttat
    23821 ttcatccatg acttcatcaa atggtttaac cgtaaagtct ttgcacagtt tattatacag
    23881 tttattaata tcttccttca tcatttgagg ttcataacat attaactcat caacggtaat
    23941 gttgaaatat gtcgcgagtt gcggtaaaaa ggttatgtca ggatagcttt gtccagtttc
    24001 ccatttagat acagaagctt tagaaacgcc tatataattt gcaagttcat cttgtgtaat
    24061 tcctttttct ttacgcttta aaatcagcgt ttttgcgatg tttaattcat tcatgtcaat
    24121 ccctccttat gtataattat atccttaaac atgtaaagct aacaagcgat ttgaagttga
    24181 cttttgacga aaaaatcaac cacagagaaa caaataaaca agtatataaa atatatattt
    24241 aaatttatct ttaatctctt ttgtaaaata tgtaaacttt gtaaataaaa tccatattat
    24301 ataaaactaa tagttaatct atctaaatac agtttttatt tatcacgttt ttaaatattt
    24361 tgtgtaaaac ctcttttaat ataaacacct tattttcatt tctcatttca tattcttttt
    24421 aaaatactga atataactct tcgattttcc atttaattct tttataaagt tattattcgt
    24481 atttattcct ctaattataa caggcaacta tcatgtattg gtatttgtct attatataaa
    24541 aatctataaa agaaaagcag tcaatcattt agattaactg cttgtacata tatcttatta
    24601 aactgtataa actataattt ttattattct ccattttcat cccaagtcat aaatccccat
    24661 ccactatctg gataagtatc aatagacttt tctctaatca tataaccaac tttataacac
    24721 tcttttgtta ttttattaga tagtatacat ccttctccat ttatctaatt ccatttaatt
    24781 aacttttctt ttcaacctta acttttttaa aatcatcttt tttaataatt tcatacttat
    24841 tttctcttca acatcatcaa atgagggtgt ctcaaaatta actttttact tcataaggca
    24901 ctcttttttg tatgaagtaa aatatatttt catcatttca acaataagta agaggcttat
    24961 ctctattttg agacagcccc atttgatggt gttgaacatt taaataaggt tgaactcgtt
    25021 ttatctctct ttttaagata tctgtaaatt tgcacataaa gtctcttctt cttttacaat
    25081 tctaatagtt ttattaaagc caaagtatat atctccatat tctttcgtat atgatttatt
    25141 ggtactcttt catctactcc atgaccacca gttttatacg ctaagaactt aggtccaaac
    25201 gccaccatat ctttaaacca acttgcatat gtgccaccac ccatagcaat tgtatatcca
    25261 ttttcatctg ttacttcttc ataggcttct aataattttt taagtactaa attactctca
    25321 tccacataat gtaagtcttt atgagcaata acctctattt tccaaccatt acaaaaaacc
    25381 tttttgaatc tatctattat gttatttaaa actatatttt taccatacct tatatctaac
    25441 tcacatacga ttttttcctc atttactttt aatattccta aatttaaggt agtgtttttg
    25501 aatacctcat catattgatt aatccccaat gcatctccat aataatcact acaaaggtat
    25561 tttgaaacta attctatatc ttctctatgc tgaaaatcca tattatcctc aactatcttt
    25621 attaccattt tagatatagc attttctccc ttttcaggat tacttgcatg tacagctact
    25681 cctttagtag ttaactctga aataattcca ttaataattt ttgcagtaca atgagatgga
    25741 acaacatttt ctttagttcc tccccttact attatgtaag gtttactttt gtcaatttta
    25801 atttcttttt ctatccttat atgaaccctt cctttttctc cattaaccac tggatacata
    25861 gcatctggag taaatcccat acttggaatt tcttcacctt tatctaagta atatttgata
    25921 catttcatac ctgtctcctc atttgtacca aaaattaatc ttattctttt attaattgta
    25981 ggatacatat cctctatgtt tttcaataca tgtataagca tagatacagc acccttatta
    26041 tctatgacac ctcttccata aataatattg tcaattactg ctcccgaaaa tgggtctaca
    26101 ctccacccct ctcctggtgg aactatatct aaatgcattg gaatccctat taattcctct
    26161 ccctctccaa tttctgcata tccaatataa ttatcaatat ttttaacact aaaacctaaa
    26221 ctagcacagt aatctaaagt tttatctaaa gattttttag gtcctatacc aaaaggacat
    26281 tgtgaaatag gttcaccttg ttgtgaatca atttttatca attcttttag aaattctatg
    26341 gttctattta aactattatc tacacttctg cttatatcca taaatcaacc tactttctat
    26401 ctttttaaag cctgtcctta aagaacaggc ttttagcatt aaaaattatt tttttgtaaa
    26461 tactaattca caaacttcat catcatgagc acatttttga gcaaaatcta atttaacacc
    26521 ttgtgcgcag tcaactcttc cataatcccc ataacaagct aatctacaaa tttcttttct
    26581 ttcttcttta gttaatccat attctttcca agcagcatct aatggacagt gatggaattt
    26641 taaaacgcca ttctcatctg tattttctac taattccatt tcaaacactt tttgtccttt
    26701 actgttatat aacatttctg ccatttttcc tggagtatct gctacgttat atttttgacc
    26761 tctcatttta ccaaatccat atattgcttc tttacatata tcatctggat tagctcctaa
    26821 tttttcagct tctttcataa gaagatagaa ccatactgct ctttctcctg ttgctcctct
    26881 aattccttct aaatattcct tttctgtata cattttttct gacatttctt tctcctccta
    26941 aaattaaatt ctatatttta atcactgtga attagaatat tccaatgaat gaccctacca
    27001 atgctaatac aactaaaatt actaataact tagttattgt ccacttcttg tatttcattc
    27061 cccaatatac gaagaataca acaccaagtg gtaataaacc tggagcaatc atatttaatg
    27121 tatcttgaac aacaattgct ttaccaccaa tatcaaatga tagcggagtt gaaagcttaa
    27181 ctgtagttgc agataatgct cccatcataa acattcctag aattgaagca ccatcaatta
    27241 tcttattgac aataccacct gatagaattt tcttaattga atctctacct aaagaatatc
    27301 ctaaatgaaa taagtaataa ccttcgcaaa atagtaatat actaaacatt attgggaaaa
    27361 atgcacctat aacacttcct tctgctccaa atgaagcacc taaagctaag aatattgttt
    27421 gaaatgtacc aaaatctaaa gtatctccaa ttccagccaa aggtcccata agaccattct
    27481 taacacctga aataacttct cctgggattt ttccttcagt agctttctct tcttccatag
    27541 ctaaaactgt accgtgaatt aatcctcccc atatagcttg agtattaaaa aattgtaagt
    27601 gtctctttaa cgcatctact aaatcttctt ttctcttata taacttttct aaagccggag
    27661 tgaaacttgc acaaactgct aatgcttgca ttctttcaaa agagttggat aattctgctg
    27721 tccaccacca tcttagatat gtttttgtta catctttctt tgtaagtact cttgtcttat
    27781 taacttcagt tggaccttgt ccatttttaa cttctttatt ttttatttct tgcattatac
    27841 ttcacctacc cttttgtctt ctttcataaa ggtaattaaa agagctatac aaacaccaaa
    27901 tattgcagct gcagtaattg atatttcaaa atattgaacc ataaaaaatc ctataataaa
    27961 cattggtaaa aatttatttt tacctatagt aaatattgtt gttgcaaaac ctaatgcagg
    28021 taatataccg cccattactg ttaatccatt cattaaccat tgtggtataa catctaataa
    28081 tgattgaact aaatctgctc caaaaaagtt acatacaaaa actattggaa accttaaaac
    28141 aaacccaaat attaaagaat aaagtgttgc acatctccag atgccttttg tattaccttc
    28201 ttcagcatac ttatctgcct tatgaaccaa tacagcatta ccagttcttc ttaagttatt
    28261 aactaatact cctataacac ctaaaggtac cgcaatagat actgcaactt ctgcattaac
    28321 accagtttga agtgctactg gtattgcaat aagagcagct agacatttat ctgatggaat
    28381 atttccccct gctgcgacca ttcctagata aaccatttct atacttgcac caacaatcat
    28441 accagttgtt acatcacccc aaagtaatcc aaggctaaaa cctataaata aaggagactt
    28501 tagtgttgag aaaagggtat accctgcaag acctgctgca aaccaatacc ataatcctgt
    28561 tacaattgcc aacatcaaaa cagattccat ttataattcc tcctaattta tattttttat
    28621 ttaaatttct ttaacgcatt ttcatatgtc atagatgatt cctctggtat aacttgtagg
    28681 actatttcaa ccccattttc attaagttca ttaagttgtt ctacatcctc atttcctaat
    28741 gaaacagctg taaacaccat tttctttcct gcttcatttg gaatacctcc taattgaatc
    28801 ttttcatatt taagtccagc tttataagcc ttatttgcca tatcaacatt tttaaataga
    28861 ataaatatat ttttatctcc taaagtgttg ttttggaatg cttgtcccgc atcttctgct
    28921 gttaaaataa ctacttctac tcctgatggt gctgccattt tatatatgtc agccataaat
    28981 tcatcttgac ctaagaagtc atcaactatt attatttttt gagcttgagc gatttttacc
    29041 cattttgtta taacttgccc atgaattaat ctgtggtcta ctcttactaa tgttattttt
    29101 gccattatat tcacctcata atttatattt ttttaaattc tttaattaaa tccacacaaa
    29161 ggttattatt ttttttgacg attctttctg gtaatgtatg taatttatct tctttgctta
    29221 attcttcatc tacgattaaa attgtctgta agctaagtcc atttattgca tctattcctc
    29281 ttcttatagc aacagctatt gcaacacttg atgtagatcc tccatataaa tctgatatta
    29341 taattttcac atcttcttta tctactactt cttcaacctc tttcataaat acagaaacat
    29401 ctttttcaga acttagtgat aaaacttcat attgatttga caatccaaag tttttatttg
    29461 tttcttctac taagactttt ccccaagttc catggcacag tatgattacc ttatatttat
    29521 tcattgctaa ataaactctc ctttatagcg attaatattt tggtttaatt ctacttcttc
    29581 ttcatcaggt accatttgaa ttgatacatt aatattcttt ttaataaatt catctattaa
    29641 aagaaactct tttttattaa tgtatacatt ttgagatatt ttcttttttc ctattcctgt
    29701 aggtattctt ccaacaataa gttttttgat attaatccct tcttctatca ttctatttgc
    29761 agtatctaaa tttttaatta atacaaaaac gttatcatca gcttgattat tataaaaata
    29821 tctccttgat tcacttacag ttaatattga taaatcaata ttctttggtg ctgccatttt
    29881 tatcaaacct ttagcaataa catcattggt tgtttcatca tctactacta ctatcttttt
    29941 tacacctaga tgcttaaccc atttatatat aacttgacca tgtattaatc tatcatcaca
    30001 tctgcaatat tttactaaag acatttttaa tcaaactcct ttttctatta cattgagcta
    30061 agaactttat taatattgtt tataccagtt tgtcctgctt caatacatgc ttctgctaac
    30121 tcttctaaac tttttccctc tctattaatt aaagcttcaa gtagcattgg aagatttact
    30181 ccagtaagtg catgaaactt gccacctttt ttaaggttta aacttgcaac attacttggc
    30241 gaacctccta ataggtcagc taatactaaa actccgtcac cttcatctaa ttcatttaac
    30301 ttttctacaa aatttttatt taagtcttca atattatcat tcctatttag tttaattgct
    30361 gctgttcttt ctgctggtcc tgcaataagt tcacagcttt ttataatttc ttcactaaaa
    30421 ctcccatgag tcataactat tattcctatc accaatttca cctcactttt cttgtcactt
    30481 tttatataag caattgccgt gccaacttat gtgtatcaat aacaaatggc tattttacag
    30541 gatttaatca aaacttttta ctcaattttt tttaaaaact tattatttcg atatgtatca
    30601 aaagattttt tcaacctgta tcaaatctgt atcaaaaaaa gtaaaaaaaa gatagccttt
    30661 tggctatttt taaattttgt atcaactttt gaatttatct gtatcatatt gtgattcaaa
    30721 tatatctaat atatttgcat attccatatc tgaaataatt atatcgaatg tctcttctac
    30781 tagcttaaaa ctttctttta taactaaata taattcttta tttctactaa ttctttcttc
    30841 atatgattta taaactaaag actcttttat tattactctt tcaatcatac atgaacaatg
    30901 aaatataaac tttactaaaa gttcatcttg aattactaaa gaatagtgtt cactaattct
    30961 attaattact tcttttaata taaaataagc cttttcccca tttaaaaaag tcaaagtttt
    31021 actaatatta tcaattagta atctatcaaa atatctacca ccattatctt tttcagcata
    31081 tgtaacttcc atatctccat aatgtttctt tatagattct ataatattaa aatataattt
    31141 ctctaatgtc atttctggag ttagagcctt tctagttgct tctattacaa taggtgtact
    31201 taccatatct attgctttta cttcttttcc tgtagcatca cgaataactt tagcaaatgt
    31261 taaaatagac cccatatcag ttaaaattaa aattcctttt cctttatcaa ttttattggc
    31321 tatttctaca gcttgtacta aaatatcctg aactctatct tctaatccca tattaattgc
    31381 cactgcatgg ttacaatcca gtaaagtatt ggctacattc accatgctag atgctgtaga
    31441 ttcaccatgc attatgacta atattgctat actctcctca tttaatccca catacgataa
    31501 ataaagaaat gttgctaaaa aaattgcttc atcttctgga aactctactc catacattaa
    31561 tgacatgtta gataatattt cttttgctat gttatattca tatgctcttt ccttcataat
    31621 ttcatcttta ttaggatgct ttactatatt tccaatcttt attctttcaa ttaaattctg
    31681 aatatgtaaa gctatagctt ttattaattt atctaatcct tctcttttgc tccatttatc
    31741 atgcttttgt aaaatatatt ttacagtagt tgcaattgaa tcattaacaa tgttgttata
    31801 agctgtttga ttactactat tatatataaa agtattcatt aaattatagg aatacttctg
    31861 tatgtctcca tctataacac ttctaatttg tgactcttta ataccttctt gttgaagttt
    31921 attccaagtt tcttttatca ttccataaaa atctaaatca tattttttat tttctattaa
    31981 taaattttca ctttcaaaat ccttttcttc tggtaaaaaa ataatatcat cttttccaat
    32041 aaaactgaaa ttttgtacaa actcctcttt tctattattt ccatataatg attctctaat
    32101 attatttggt agaagagaga gccttacata tacacattcc cttttatagg tcatataatc
    32161 taaaaaagct cttgcacaaa ttaattgtat atcgcttttt aattggccta tatttccctt
    32221 acacttatat aaaataaatg ccttaataac ttcttttgaa acctttaact taacaccaat
    32281 cctaacactc tcttctttaa aaaaataaca tataagcttc attctctctt gaaagcctct
    32341 ttcctctaaa gacggaatct ttatagttac aggtattctt cttaaaaacg tttccaacat
    32401 tgctgtttct gggtcttctg tagtggctgc aattatcata actctacact cgtgtacctt
    32461 attagcctct cctaaccttc tatatatccc tttatccata agcagaaata gcatttcttg
    32521 tccctctgca gataatctat gaacttcatc taaaaacaat actccattat tagcttcatg
    32581 tacaatacca tacttatctt tatcagctcc agtaaatgct ccttttacat atccaaaaag
    32641 ctgcgctaat aataattgct gattatcaga ataatcagca cagttaaata tgataaatgg
    32701 tgcattatca ctatcttctt ttatagttaa actatactta tacatatatt ctgcaaatat
    32761 agatttacca actcctgtag gtcctattat taatgtactc aaccccttag gtggatatat
    32821 tactgctgct ttagcctgtt ctatttggga ccttaaactt ccatcacatc ctactaaatt
    32881 aaaaatattg ttctcttttt ttatactctc taaaatatac tcatctttga tagaatcatt
    32941 ttcactatat tcgtttatag agttaatatt atcaaatatc cctttattta gtttctttcc
    33001 taaaatactc tcaatacttt ctttatataa gtactgaaca ggcttaccct ttatctttat
    33061 aataatactt tcttttatta attgatttaa ttccttacta gcattacttc tatttatacc
    33121 tacttcagta gctatatatt ctgcatcaaa tccaaatcta atgccattat taaagttcaa
    33181 gtgttcttct gttaaccttt tcaagcaaat taaaactttt tctttccttg atagcttctt
    33241 ttgtgtcata gttaacctcc cctataagca gtaatactgt taaacattta ttattaagct
    33301 taattctaca atattttaat atttttgtct aattatgtaa ctattgattt tctttcctat
    33361 tcgttttata atataaatta cgttaccctc atcttttata gaggaggaat ttatatgaat
    33421 tttttatcag atttacacac acactctata gtcagtggtc atggctatag cactttacta
    33481 gaaaatatta attattgtaa agaaaatgga attaagatac taggtacatc tgaacatggt
    33541 cctaagatgc ctggttctcc ccacaaatgg tactttcaca acattcaaaa tatcccaaga
    33601 atcataaata acattataat tttaagaggc tgtgaagcaa atatactaga tattaaagga
    33661 aacatagacc tagagccttt tgtaattcct cggttagatt atcttatctt gtcatttcac
    33721 gaagctgtgt tttctccaaa tacattagag aataatacta aagctttaat aaatgcaata
    33781 aataagcatg ataatattga aatattagga caccttggaa acccaaacta cccaatcgac
    33841 tatgaattaa tcattaaatt agctatagaa aagaatatct taatagaaat aaataattgt
    33901 tctattaaag gcgtgtcaag aaatggtagc tcggataact gtaagtatat tgctacactt
    33961 tgcaaaaaat atggtgctaa aatcatactg acttcagatg ctcacatttg ttttgacata
    34021 ggtaactatg agtattctga aaatatctta aaggaaatta attttccaga tgaattaata
    34081 atgaattatc ctaaaaaact tattaatcat tttcaccaaa aaggtaaatt actagatgtt
    34141 gattatacaa atatctaagt aataatacat gcttacttta actcccataa aaattttaat
    34201 actatttata ttatatgaaa taaagaaatt ataataaaga agctatttaa taaagcttaa
    34261 ttacttttat gaacttgatg gattgatatg gtctatgata gcaatagaaa ttataacatt
    34321 tttaataaac ttagttttat ataccttata gtataaatct aaaaaagcat catctaaaaa
    34381 ggagctgtca catgagcaaa ttaaatttgt tctatgatag ctcttttttt acttatttta
    34441 attaggtata tttttaatta ttttatataa agcttatgtt ctaagtaaaa ttatgcataa
    34501 caaacaaagg tataaaaata ccattttttc agataatatt taattataaa taataaattt
    34561 agaatacttc ttttaaatga tgagagtcta gaaattttta tgtgtattct ttaaataaaa
    34621 atttccagac tctagcccct ttttgaatat tttaaaattt aacttaaact aagcaaattc
    34681 tataatagct tatctctaaa tatttaaatt taatttatca tacaatatta cttattataa
    34741 aacttaaaac atataaaaat atattttaaa gcaataattt tattttactt attaaatatt
    34801 tggagttatt aaaatctttt ctatctaaac aaagtataaa tttctaatta tctttttata
    34861 ttttcttaaa tgctcgtaaa gccttattct atgtgctttc gagtattttt actgtaggaa
    34921 gatacttcac gtttctttgc atatttcctc atgtcttagc tgtcagaagt ggtaaataag
    34981 tagtaaattc atttgtacta ctaagcaaca agacgctcct gttgcttctc tttattcaag
    35041 cgtttcattt ctgccattgc agaatcgaat gttgcatgtg cgtaatagtt cagcgtcatg
    35101 gctatattag catgtcccat aatgtactgt aatgcctttg gattcattcc tgcatttgca
    35161 tagttggtac agaatgtatg tcgcaaacta tgtggagtga tgtgtggcaa tttatcctcg
    35221 ttatacttat tgtatttctt aacaagacct ttcatcatgc cgttgtaatc acttgccact
    35281 tttggatagt tctttctatt aagaaagagg aaatcactat atccatcaat ctcaacacgc
    35341 ttatcattct ttcgattcgc taacactcgc ttaaatgctt gataggcttc ttcaaccata
    35401 ggaacttgac gttcgccact tttggtcttt ggtgtttcaa tgtagtaccc aatttcagta
    35461 tctctcaata gctgatggtc tatattgaca agacgattct caaaatctaa atctggaagt
    35521 gtcaaaccac caaactctga aatacgaaga cctgttttta agagtatcag aatttcatca
    35581 taatttttgc tgtaggtttt atcagctttt gcaaaggcta acagtttttc ttcctgttct
    35641 tctgttagta cggtcttagg gacagtatca tcatcaagaa ctgctttcag ttgaaagtca
    35701 aatggattct tccgaacaca atcatcttgt atagcaatat agaatgaagc ctttaaagaa
    35761 cgtttgtagt tattgatggt ttgataagca taaccatttt cactcattct aatagcccat
    35821 tctttagcgt ctgatggctt aatactgtca atacttctta cacctaactt gtctttcttc
    35881 aaaatatcca taagatattt gcgtccagtt tcagtgtttt ttctaacctt tggtctttga
    35941 gcgttctgtt ttgcgtaaag ctggcagagt gtcattttct ttcctacaac atcaatacca
    36001 tcatgaatgt ctttctgtaa ctctgcgatt ttctctctaa gtgagataca atcacgcttt
    36061 cctgctggta ctcggtctgt agccacaagt ttccacgagt aaacaaattg cggttctcca
    36121 aatgaatcta tatatttgta taagtatctt ccgtcttttc gttggctctc tccagtcttt
    36181 aagattcgac ctttattgtc acgtcttttt tctgacatgg catttgctcc tttcctttat
    36241 ggaaagagcc ttgatacgac ttaatactat tttatcatat acaagaccct ttggcgacgc
    36301 tagattgcgt ccaatgtatc tataattttt tcaaattgtt ttcgtttaat ctgaatacga
    36361 ttgccattca taatcagcca atttgcattt ttattttcct ctgccaagcg tcgtagcttg
    36421 ttttcgccaa tacgaaaata ttttgacgct tcttcaatgg ttagggtata acgttcccaa
    36481 ataggaatgt cagtctgctt cataaaatcc tcctttccaa atcacttatt tggatttcat
    36541 aaaagttgtt ttaccagcaa tcgaacagct ttagcaaagc tcacgggagt tccacccctg
    36601 catggttctc atgtagccat actcattgcc tgcgacggtt ttatcacgct cggactattg
    36661 actgtatggg agtatcatta tcacgataag aatgtcgttg caggcaatcc tgctaaagat
    36721 tgcttctcgg atcactaaca tgaatcgctc gctatcttta taagataggt catggcggtt
    36781 agttccgttg gctcttttct tatcgaaacg tattcgatta cttttattca gttttcaaag
    36841 aacaatggct cgttagccta tcaaaacaca ttgaaagctc aatatgcttt ggtggaataa
    36901 caaacctccc tgttcgggaa gcgtggaatg gtttagcacg cttccacgaa aggagagagg
    36961 atattactta atttcaaatg acaaaatctt tgtaatcagt ctggtttcca ttcttccacg
    37021 taagacttca tcaacgacca tactttgatt gccatattca tctttcataa gtcgtaggga
    37081 acgcttcgtt atgtaccctc tgtaatgatg tagaatctgg ttaatcgctt cggtatcgcc
    37141 atctgttgcc tttacaatga gaggaaaggg aatcatagga tattgtgttt tcattcttca
    37201 aattcctcca taaacttttt aattaaggct agtccactgg ttctatgccg atagacagta
    37261 gaacggttca atttcaacag gtctgcaatt tctgaatcgc tcatgtccat aaagtaaaac
    37321 agcagtagaa tttcacgttt cttgtctggc aactcacgta atgcttcact caacaaatca
    37381 ttttcaacgc ctactgataa cccattgagt gtaaaaatct gaaagtcagt tgaatagtta
    37441 tctgttgtcg caaactggct aacaagataa tcgccaacat ccgaaaagga cacctcacgc
    37501 tttgcaatcc ttgaaagata aagcatataa ttctttcgct cgtcttccat agcacgttta
    37561 cagatatagt caaactgatt ttctattgtg gtctgaaaag aagatggttt catgtttctc
    37621 accccctttc tgtctaggaa aggaagtgag ccttgctcgt ttatctcctt tcactcttag
    37681 tcccaatgtg aaagggggat ttgttgcatt actgataaat aaactttgta aaaaagttct
    37741 gaatagccaa aaaagcatat aaacagattt atttctctgt ttacatgctt ctgttattct
    37801 atctatatga tttataaaac cacattggtg gacgtactta tctattgcag atagacgact
    37861 ttttttgaca agaacccaat gtaaggaaat ttattgtata tgatgtactt catggcgacg
    37921 ttgacctcca acaaaccgcc atttggaagt aatatacaat attttaacag cgtaaatagc
    37981 actaccatat aacggttttt tttattggcg tttagtagtg ctttttatta aatataaacc
    38041 tataaaccat ataacacgtt tttctatacc tgtttttaat tcagtaggaa caataaaatg
    38101 tatagaggtg gtctactatg cgtaaaaaag aagataaata tgattttaga gcctttggtt
    38161 tagccattaa agaagctcga ttgaaacgag gtttaactcg tgaacaagtg ggagcattga
    38221 ttgaaattga cccacggtac ttaactaata ttgaaaataa agggcaacac cccagcatac
    38281 aagttcttta tgaccttgta tcgttacttc atgtttccgt tgatgaattt ttcttacctg
    38341 ctaataactt ggtaaaaagc acccgacgat tacagataga gaaatacatg gatagcttta
    38401 cagacaaaga actatcctta atggaatctt tagccagcgg tatcaacgaa gcaagaaaca
    38461 tcgaagacta attaaaagaa tccatacata acggaaagag ccgataaaat gagattgtat
    38521 taatctcatt ttatcggctc tgcgtctttg cgtctggctc tgtaatcaca gttactttga
    38581 actgctttat ttcaattaaa ttttcttgtc tgcatttcgg acaatagagg gggaattttt
    38641 ttaattcagt atcttccctt atctttaatc gtgttttatt tccacataca ggacacaata
    38701 tccacttgta gtttataata actatctcct cctttacact ttaattcaaa tctttattaa
    38761 aaaatatttc atcttattta acaagaaacc atatttatat aacaacataa aatacactaa
    38821 gttattttat tgaacatata tcgtacttta tctatccgac tatttggacg acggggctgg
    38881 caaacaggtt caccggtagt aacatggtac ccttttaact ctgttaaaca aacactacgt
    38941 ccatttgtaa agaaagttaa atcactacga tattcttgaa tacaccgagc agggatttct
    39001 ccactaagaa tgacctcatt atttttcaat tgagtgtcta cgatgttcgc acaatattta
    39061 ggagcatcgt tgtatgctcg tgaaagatat tcctgtggcg cataaatttt aaaactaaga
    39121 tatggctcta acaattctgt tccagctttt tttaagactt gttccaatac aataggagca
    39181 agcatccgaa aatctgctgg ggtactaaca gggctatagt ataagccata cttaaaacag
    39241 attttacagt ccgtcacatt ccaaccatac aatccttgtt cacaaccata gcgtatccct
    39301 tccataactg cattttgaaa tgattgattt aagtatccaa gagaaaccga gctctcatac
    39361 tgcattccac ttcccaacgg aagcggtgat acagataaac caatggaagc ccagaaagga
    39421 tttggcggca cttcgatgtg aatggtatat tctgcatttt ttaacggtct ctccatataa
    39481 atgactgtag gctcttttag ttctatctcc acatgatact tttcttgcaa cagtgcacta
    39541 atcacttcca tttgtacttt ccctaagaaa gaaagtataa tttcatgtgt cgtagaatcc
    39601 acgtaatatc gtagaagcgg atcactatct gagatttcca aaagggcatc aagcaacatt
    39661 tctctctgtt caggtttact cggttcaaca gttgtttgta gtagagggtg cggattttca
    39721 atcttttttc tctgtggcaa tagttttgta tctccaagaa cactatttaa cttcaaaaac
    39781 tcattttgca aaataacaat ttctccagaa taagctctat caatcttaca taattcacca
    39841 tttattgaag tatacatttc tgtaactttt attttttctt tttctgatac tctaaccgaa
    39901 tctcgtaaat gtagtactcc actataaagg cgtatatatg caagacgttg tctttttttt
    39961 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
    40021 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
    40081 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
    40141 tccaatgctt ctaatgattt accggacata tatttctcta aaaggtcatc gtttccctct
    40201 attaccgtat cccattgttc agattcggta aagttcgtca cacacacatt aggatacagt
    40261 tctaccttct gtttgattac aatttcggca gaaagtttct ctttaatatc ctgataaacc
    40321 gttgataaat caattccatt ttggtcaatc ttattgataa aaaagattgt gggaatcccc
    40381 attttcctaa gtgcatgaaa taatatacga gtttgtgctt gtacgccatc ttttgcagaa
    40441 atcagtagaa ttgccccatc taaaactgat aatgaacgat atacttctgc taagaaatcc
    40501 atatgtcctg gcgtgtctat gatgttcacc ttcgtatttt cccactgaaa agaggttatt
    40561 cctgtctgaa ttgtaattcc tctctgacgt tctaaaagcg tattatccgt cctcgttgta
    40621 cctttgtcca cgcttcctaa ttctgtaatc gctccactgt tatataataa gctttctgtt
    40681 aaggtagttt ttcctgcatc aacatgagct aaaactccaa tattaataat tttcatgtga
    40741 ttttcctcca ttcaaaagcc caaaagggca taaaaatccc agtgataaat actcttatca
    40801 ctgggatttt tatgcataac cataggcata caaagcatac agatattctc cggatacttt
    40861 agaatcacat gataaaggta ttcttaaact gggtacaaaa aactaagccc tcctaaaaaa
    40921 ggacatccaa ttatttgttc ccgctatcaa attgacagtt tatttaataa taccttgccg
    40981 catatttatt aactcctttt aaatagatac ttaaataata gcacgtaaga gcatatttgt
    41041 aaaggaatct ccaatttttt atcaaagaga gtacgtgatt acaaaatagc tgtaataatg
    41101 taccaatatt tgttattcta taatcttcca attactcccg ttcttttcaa gtaccaaatc
    41161 aaattgagat acctgcgttg ctttggtctg ctggtcgata tactccactg tcagcgatac
    41221 cgtgacttga ttatccttac gattgtgaat aggatttacc agttcttgaa agatgtactc
    41281 ttttccgatt ggttttaata tcccgtcatt cacatagtag gaaagttcac tggctgtcgc
    41341 tgtaggatag agcttgaaga acgtcgttaa aaactcattg atttcattgg ttgtaatgga
    41401 atcaaccgtc ccctcacttt caatggcttt tggtttataa cttgatttct taggtatgtt
    41461 ggtaatggtc ggattcttaa ccagtaccat atttccagaa ccatctacat agacactcac
    41521 tatataagca gagtggacgg tctttgtatt ttctccctct gtaatgagct ggtctacact
    41581 gtaggttaca ttaaactcat tgtcgccagt tggctctacc gtccatatct gaaatcctct
    41641 tacagaagac gatacaggaa tatctttgcg tactgtatca acattgagag cttgaagttc
    41701 atctgtcaga tagcctttta gactttccat tcgattatca atggacttat cggattgctc
    41761 ccatgaatag tagactttcg caaagttctc tacaaaattt tctacatgat gagtatcaac
    41821 gtattccttt tctatgatag ttgtttcgtg aatagtatga gtatctatag ctgtaaagtg
    41881 cttgaatatc gcaaagctga aactaagccc taaaagtacc cacaaggcaa tcacaacctt
    41941 tttatgagga ttgaccttat agacacgagg tttcttttcc tttggtatct gtttttcttt
    42001 attctgattt tttctaaatt tcatcattaa atcttccttt ctcattgttt gattcgtcct
    42061 gctcccacta aatgctgttg ccagtagggg cttgttaagt cggcataacc gattgggtcg
    42121 cctgcatgaa acatacggtt attgccaagg tatatcccaa catgagtaat ataagagcca
    42181 gcgttatagg tagaatgaaa gaaaaccaaa tcgccagctt gtgcttccga tagtgggata
    42241 tgctgggtca catcatattg ctgttgtgcg gttcgtggta agttaattcc agcttttcca
    42301 tacgtccatt gtgtcagtcc gctacaatca aaagaagtag tcggggaagc tccaccgtaa
    42361 acgtatcgcc agccctcata tttcagtgct tcgtccatga tggcttgtac cgtatcatca
    42421 tcaaactctg ttgtgacaag atactgcgtt accagttgca cataaaacat attgccatag
    42481 ttgtatcgcc agcccccatt gataggtatg gctatgggat tggggtaaga cactttttcg
    42541 ccacctgaat actcttttga gaaactttga gccagttcaa aggtatattt atttccacga
    42601 ttagccacat accctaagaa accaccacca taattgtagg actggataac cgattctaaa
    42661 tctacactga gcctttcgct actggctaat aattcactga aatacttcac accttgctta
    42721 atggattctt ctgtactcaa tgaattaggt ggaagaccga gggattccga ggactgcata
    42781 acatcttccg cagtaccgcc cgattccacc tgtataatcg caagaagtat gttgacatat
    42841 tcttcaacgc catattcttt ggcatatttt tctaccatag gcttatgagc cagcacttct
    42901 gcggaaacat tcacacctcc ataatgaata ttggaaattc cgctgtcctg ttcatctgaa
    42961 aataaaatgg caacaaacag aagcagtgag aagaccatca agaataatcc agaaccacca
    43021 atcactaaag ttttcaactt catggtttct taccgacttt cttaatggtg gcggttttga
    43081 ttggtggtct acttcttgta ttttgtagtg gtactctttg aacagtagac ggacgttctt
    43141 ttgtgattgg acgttgtgaa gttctatctg ctgtagtggt tgaagttgct ggcttttgaa
    43201 cggttttttc ttgaacggta ttgccttggc gttccacttt tggacttgaa aaatcggact
    43261 taactgctgg acgctcttgt ttggcttgtt gagattcctt atatgaagtc tgaatattag
    43321 actgttttga ggtctgttca tcatgatatt gttcttgtct tgtagtcggt ctttcatgaa
    43381 cagaagaagc aggctgtttt ttctgtttga cctgttccat ttcagagcga cgcttcgcaa
    43441 tggtttttcg cctttgttcc tgctgttcct tgcgtccact ggctctgtcc gctttggttt
    43501 gagaaatact actggttaaa tcacggacat tctcttttac tttggatttt ccttgatata
    43561 ctgcatatct tgcattggtc ggcaaatctt taacctgttc tttcaaacca ctagcagtgt
    43621 ctaccattct gtctttggta tcagctactg taccgatggt ttgaccgata cgttttccaa
    43681 gtgttgattt ttcctttccg tctggtcggg agtgatctgc ttgtgtcctt gcagaactcc
    43741 ccgaacccga ctgtcctttt ttacctgtaa caatggcaga cccagcccct agagtagtca
    43801 tggaacgtcc aagtttccgc tgtagacggt gcatgtgagc gtgcataagc atacgaggtt
    43861 ttctcatcac acgacttccc acactttgag aatcgttact ctgtagagaa aacatactca
    43921 ttaaatcgcc cagcttgaag tagattcctg caaaggtcac aatctgtaga aaagcaatca
    43981 aaaagaacgg ataaccagcc gataaggtat agagcatggt tgaaatacta aatgctgtcg
    44041 taataatcaa tgtgattcca gctcgtgtca aaatggtatt aaagagcttt gttatggctc
    44101 gttttgacat accatcaaat gatggaatca tgcttaaaat aaagctcaca ggcagaaaca
    44161 tagcatagat gataaaaagt acctgcgaga aaatcatgat tcctgttaat aggaatacaa
    44221 atatggaaat cccaatattg aagacaaata ggaagaagac tgtacctaaa cggttaatgg
    44281 tctttgtaat ggttagattg gtattgcttc tgtcttcaat ttcttccgca acaatttttt
    44341 ctctgtcttc gccattgttg gaatctgggc tggtggagag caggctttcc acacggtcaa
    44401 taccgatact ttcaatgtct gaactgttgt attgaagcag tagccacggt tgctgaacct
    44461 gtatggaaaa caggctatct ctgattaagt ccacgctgtc cttgccttga ctatcggaat
    44521 ggggcatgac aatcttcgtg ccaagtgata aactggcatt actgatgtct gatgaaaagt
    44581 cattgatttt tttaatgtag tcgggagcgt aggcaataaa ggaagccgat aggataaaca
    44641 ccagcacaaa attcataatg gcatgaattg cctttgtggt ttctctcttt atcagtcccg
    44701 tataggcaac ataaacccca agaaccaaaa tcaagagtaa gaggaatcca acatagaaac
    44761 cctctgttga aaatccgttt gcactcacac cagctaaggt ctgcatattc ttaccaatgg
    44821 aatctgctgt agcggaaatg aagtctaagg aataggcttc ctgtactaag taacctgtcg
    44881 cattggaaac atacaaactg attgtccaaa taaaattggt aatggcatat agtccataca
    44941 tgacctgttt tccaatcccg tccgaccagt tccacggaag ccagccccag ctattatcca
    45001 cataaaaatc cagttgatag ttttcaagtg ggtatcggct gtattcattt gccacattga
    45061 ccgtatcatc taccaagccc gcagcttgaa ccaccgttcc cagcatggct aaaagaaaaa
    45121 tggcaatcac aagtgtgaaa gccactgtca ttgccacttt acctagacgt ttcagcgtcc
    45181 agtttgattt tattctgttt actattgatg gtttcacatt tacacctctt ttcgcacagg
    45241 tggtctggta tcaaaggcat ggagcagttc ttcaaataca gggtggaact gtatcacacc
    45301 gacacgacca tataaatcac tgataaggca ttgcccgttt tccaaatcac gcaatcgctt
    45361 ctgattgttt tcgtcctctg ggtctacacc aaaaaaggct aaggtctttt taatctcgtt
    45421 aaggtcagtg gaacgaaatg caaattttaa gccgaggtta tttttcagtt tttcatctaa
    45481 gaggtcgtct gtattttggg tcacgaaata taccccagcg ttcatagcac gaccagcccg
    45541 aaccagcttc atagatagtg tttttccttg tgctacctgt aaaaagctcc atgcttcgtc
    45601 taaatctaca atcttgaaaa tgcttcggtc tgtatggata aagtctaaag caaaggtact
    45661 aatgacaatc agcatagcaa cggataaaag ctccatagtg gtatattcct caaaggaagt
    45721 ttccttgtcg ggaagtacca agtccgcaac ctgtataatg ttcagttgtt tttctaagct
    45781 gatagactgc tccacataac cattactgaa taataaatgt gcaaagtcat agtctgtaaa
    45841 actttcgata tggtcggcta tactggtact tagtggcgta ttctcaaccc gtaattcctc
    45901 aatcactttc atcaaccctc gtacttcact attggttact gcacgaatgg cttttctaag
    45961 gattgggaag cgttccccat cacgagagga aatccccgta aggaatgtca gaatatcaat
    46021 agccagtgat tcagaatctt tgggattttt cataatcaca taagggtcaa gtaagccttt
    46081 gtttttctca tcagaagtca gagtgacgat attgatttca tgggaaatct ctggcaaggt
    46141 ttctttccat ctgccacgtt ctgcttttgg gtctacaatc actgcttgtg ccccataaag
    46201 caccgcataa tagacgataa ggttattcgc aaaggattta ccaccaccca gcgaaccaac
    46261 aaaagccgac gctaacgcat tggttactga acccttaacc ccttgactgg caagagcagg
    46321 tttcagatag acattgcgtc cagtatctaa gctgtagcca acataaatcc cctcattttc
    46381 ccccagcatt tgagtagcac caaaacctaa accagcgagg aaatcagagg tcacgtattg
    46441 aatataatca ttcatataac gcttgctggc aggtaaaaat tcttcatgta agccgagcat
    46501 atccccaaat ggtcgtacca gttttacgct taaatcgtca taaaaatctt tcacttcatt
    46561 acaacgacgt ttgagttcgt caagatcatt tgctgatacc cttaccacat aagacagctt
    46621 gtacatagat tccttgcttt ggtctaaatt ggtttccagc tcattcacac tttccagagc
    46681 ttccgccaca ttggagctgg tttcattatc actttgccaa gcgtggttat ccaagtcttt
    46741 cagttctttc tttttattgc ggacagtaga tagggcttta cgattcgcta caatttccac
    46801 attcattgac gtatcaatcg ggaatgtaaa ttgctgttgc tggtagtaga agatttcaga
    46861 ggacgggaag tccagttctc cgacaatgct gttaatggta aagtaagcta catagacggt
    46921 ttcatcttcc tgctggattt tcaaatatcg ctgtttttct tccaccaaac agcgagtagg
    46981 cttaatcaag tcatagtatt taatcagcgt ttcattatcc agctttttct ttgatagatg
    47041 gtactcatac tcttcatagg cagtgcctgt ctgtccgtaa aggtgttcaa tcagatagcc
    47101 gaagtcgtcc ttatctaacc tgcggatttt gaaacgacga gagattttat tttctaagag
    47161 cttttccatc ttctgaaaac gcaggatttc atcattactc atactaacaa aatcgcccat
    47221 cagcttatgg ttcacatcat agacaaaatc agacaaagca ttttttgctt caacggtaag
    47281 acttttcata gaaaactcct gatcgttgag aagcaactta aagccgataa agaaacggta
    47341 gttcacttga ttttcgccaa tcatggatat taaagcgtct gtctgttggt cgattttgtc
    47401 ataggcaacc gctttgagct tgccagtgac ttcatttttg gaacgctctt gtgcagaacg
    47461 tatgctggat tctgtactga tttgtaaagc atgaattttg ccatcacgat tttgtgcgat
    47521 aagctgtctg aaagaatcat gcacttgtat tttctgttct ggacttagaa atgagtaatt
    47581 gtaaggaaca agctcatagt aagcataaca ttccccgtct ttattccaga cgagattgtt
    47641 ttcaatgtat ttaattggat atgccataaa attcactcct aactgctgta atggcttctt
    47701 gtggctggtt tctgccaagc gttacttttt ttcctgcata ggtcagcttt ggtcgcagtg
    47761 cataagcaat gacagacttc aaaaatccat aaggcttttt accatcaaaa gtttttgtag
    47821 acataaacca tgtgaaagcc acaggaatcc caaagtattt gagaaatgct ccctctatca
    47881 tggaaagagg gggcaagttg ccaagtatca tcactgcaaa gagtgacacg acaaaccatg
    47941 tcatttgcgt aaaggttatg ggaaacggaa gtctaaaatc attgatagaa tacagtacct
    48001 tttccacaga ccagatactg gtatagcttc gtattttctt catgtaatca atcctttcat
    48061 aaaaaatagg ggtagctgat tgagccaccc cgtaaaatag aaaatctgcc agtagtaatg
    48121 taccgacaga tttaatagac gatttcaaaa atcccatgat tggttgagat aaacgttcct
    48181 gaaaggtcta aatcccgacc ataggcttga taatcaatat agttttgaag actagctggt
    48241 acttcgccta aagcacccgt ttcttcaatg tagtagcgtg ccacgtcata catatcatca
    48301 caatcggaat gaatgataat atcctcttga tgttcgctta gttcttcaat gcttgaaaaa
    48361 tgagtgagca gagcagatag ctccgattgt aattcttcgg gtaattccga taccatttcc
    48421 catagtcgat tgagttcgcc aatggaagtg tattcgtcaa ccgtaaaggg taactcgtag
    48481 tcatgaatgg cgtattcctc atattcatca ttcaagccga ttttctcttt gacttcctca
    48541 aagtcaatgg gaaaggtaaa ccacgcaccg accaattcgc cctcattgta tttgcctaaa
    48601 ttcgcaatat agacttgcat atcgtccata tattcacgtc ctttctttgt agagattcaa
    48661 aaatccctac cgcacttcgt ttggtgtacc attcctttgc ggaacataag aaaaccactt
    48721 atattccaca aaagaacggt tttatttaag caccaataat gcgattgaat agctctagta
    48781 aaatgtcttt tactccagca gcgttgaaga ctaagccaac cgcaataatc gcaataatta
    48841 aaaagccaat cagtttgcta aactcacgct tgaagccaag atacaagcca atcacaacga
    48901 ttgctaaaag caccagtgat tgagcgtttg atagaaacca gttataaagg ttttgtccaa
    48961 aattcataaa aatgttctcc tctctatatt caatgaattt gtatttgagt tatttttttg
    49021 ttgttatcac gtcctgttct tttactgact gttgcttcaa aatctgcttg tgtcggtctg
    49081 tcagtttcgc atggtcgaga atgtctttta caacctgcgt ctggttgatt tcatcaagtt
    49141 taatcgcaac ctttaaggtc ggggcaactt gatgagatag ccagttcagc gtcctttgga
    49201 aggagtaagg ctctggtttt gtggttagtt ttaatcgttc acgattgttc ccaataaacc
    49261 aagcccattc ttcattcagt ttccaatcag aacgaggttt ggaatcgtct ttatctacaa
    49321 aacggatata ccgattgata attttaaagg cggtatgctc tggattgtca tagacgagta
    49381 aatcacggac tgcataatag gcacgctcat ttttcaatcg aatctcaaaa cggtttttta
    49441 cttctgcgtc ttcaatggga atatcatttt tcttgtactg ctcgtagtcc ttttcataga
    49501 tacagaaata aacttcactt tgtaatgaac cgatatagag ggtgtttccc atacattcct
    49561 tttcctcttt gcgtaccagt tcgccactgc gatagctttt aaaactgcgg aagacggaga
    49621 tacattcttc ctgttggcac ttttcagtga gtacagggat attcaaaatc cctgtcttat
    49681 cgttaatggc aaggtcaagg cgtttcatca caccgccagc caccaaaacg tccataaaga
    49741 actcatacca gcttctttgt tgtgccagaa gatagctttc aaattgtctg cacccacgac
    49801 ctttcaattc caccagaact cctttgtcca gttcatggga gcaaaggacg aatatgtcgc
    49861 ctaaagcata atgctctgaa taagaataga aaccatagtc ctcatgaaga aaataggaca
    49921 gtttcagttg taagatgttt tcgaccacct gctgtacgtc tgttgtcgga aagcgaatcc
    49981 ttacataatc aaacagcatt tcaaggggag cgtcgggatt gaagcgttcc agagcttccc
    50041 aaagggactg ctgtaaatcc tctgatggct tgacttttcc tgtttcaata tcgcttagat
    50101 actgccttgt aataccagtc gcaacagcta aacggttttg agatagtcca taagccaagc
    50161 gtttttcttt taaatgctgt aaccaagttt gttcattcag taaaaatccc tccaatcaaa
    50221 aaggcgtatg tcaactttta aagcccattt gacatacgct gaaattttgt aaatcccttg
    50281 taaccaaagg attttctaat gtttttttga ctgtttcctg tcgatttgta cccccctgtt
    50341 agatacgggg ggttaagtgc tggcgtggct attgccacac cagccagcaa gatcagtcca
    50401 cacctgcgac ttccgcttcg cacgtcgcct gcgtggactg tctgctgttg gataactttt
    50461 taatttcctc caagaaatca tatccttttg gtacaagggg agtataaaac tctgatatga
    50521 cacttgttcc tacatcaaca tagccacgac ctttgattcg ctttaagaag aaatcctttt
    50581 gtacgtcact gccaaacatc atgccatagc ccatttcaga catacgacct aaagccactc
    50641 tgaaattaaa ctgatcacgg attccgtcgc ctaaatattt tgcgtctgga cgttgacaag
    50701 ccagtattag aaagaagcca gcttgacgac ctaacatgac aatctgtttc agcttattca
    50761 taactgcggt gttttctttt gttcccagca tttccatgaa agcgacgtat tcatcaaaga
    50821 ttaagaagtg tgccgggaga cctaagtaag cataattttt gccagtctta tagttcttca
    50881 tctgcttcat ttcctcacta cgtttcatca tttcttcata gaatgtttca atgcaagaaa
    50941 gcaagtcttc ttttctatag tagacatttg ccatcacaga acctaagtcc gcaaggtcag
    51001 catttttcgg gtcaagaata tacagttttg aatctgtatg aagcaaggct tcaatcagtg
    51061 tcagtataaa gtaagtttta ccgccacctg taccaccagc aatcaacata tgagggagct
    51121 tatcatattc ccaccatacg tttttcatta agcgaagttt accatcttta gcttctactt
    51181 catcaataga aatacgactg gctatggtgt catagagcaa agtatattcc acataggaat
    51241 cctttaactc tttatccgtc agctcacagt acaagccact ctctaatttc ttttccaagt
    51301 gtaagagttg gtcttgatat tttcccagcg tgatttccac ccgtatctgt atcaagccat
    51361 ttttaagtcg ataatacatt ttagggaagt aggttatctt ttcctttgta cgaccagcac
    51421 tatctttaaa gaaaccctct gttttgacct gttcagattc ataccacttg ttttcaagta
    51481 tcatctttgc cagtttttga cggtggtaaa gttgtttaac cgtatcatag cgaacccgtt
    51541 tgaatacaaa cgctaccagc aagcagataa gaattgcgac actgaaactg ataattaaat
    51601 agggaatgtc aatcttatct gcttgtgata ggttaaaatc ctgccagttg atctgctgga
    51661 ttgtcttcac atgaaacagt ccgacaacca gcaggaaaac aggcaggagt gacgctatcg
    51721 taaaatgaaa gactaaatct ttaccagatg ggcgaatcct tttaccacgc tgtttcatgc
    51781 gaaaaagtct cctttctacc tagcgactat ttgtcttgtg tcggttcttt ctttgcttgt
    51841 ggttgagctt tgaatgaact agaatccttt gtcagcacaa tatcgtctgc cttgatatac
    51901 cagtcaacat ctgctccttg ataggtggca gtagcaacgg tgtccgcaat gggattgata
    51961 agttccaccc gtgcgttata atcaaactct ttcaaaggca cgctggcagg aatacttact
    52021 tgaatcatgc gtccttgtcc tttggatttt aagtcatagg tacgttcctt gatttcatct
    52081 gaaaccgacc cgtcttcatt ttggattctc acttcacgac gtagagcaga gaatttcaat
    52141 tctccaaaag tcgtgtcttt atctaataca atgccatttg ctaatctcat catttttcct
    52201 ctctttcttt attcttttat catgtcgtca gcatgtaaaa ggtaatttgt aaaaccacga
    52261 gtgccgattt tgtagccctc tgcggtaata cgtggattga ctaacttcac acgttcctca
    52321 aagccgaaat gtttttcgcc agcttcagca ggaagcacca ccacaatatc atctgctctt
    52381 tgaacatcag aatagagatt atagcttctt gataagacag ttagccgtcc gttgattctt
    52441 cgctgaacga ctttatcctc gccagcaaat tctaaattgc cgaatgtttt ttccatgttg
    52501 ggaatcacaa atttaagttc catattttta cctatccttt cttttttatt ggctgaatga
    52561 atgtttgatg gtcttaaaga gtggggaacg accttttgat tcttgatttt ttgttttcat
    52621 aagttcactt cctttcaaaa tcgggtaaaa aaatagacac ctcatttttt gaagtgtcta
    52681 cctattaaat attcaaattt tattggaagt atctttatat cttcactttt caaggataaa
    52741 tcgtcgtatc aaagctcatt cataagtagt aaattagtag taaattgagt ggttttgacc
    52801 ttgataaagt gtgataagtc cagtttttat gcggataact agatttttat gctattttta
    52861 tgtctaaaaa atattgatat taaatatcta ataagaaaat ttaataagaa aaatatagat
    52921 tatatcaaaa aaatattcta ttataatctt tagaacaaac agtagtaaat gttatcttca
    52981 taaacaaatt tatttttcac aagtacaata cttatttaaa aaaggaggtt taatatgtta
    53041 agtaaaaaaa atgtaagttt aattatttta gtaataattt taagtttatt aactgctata
    53101 tttattttca ataataaaaa tactaacaat aaattgaaag aagttactaa gaataatgaa
    53161 gaaatagtac ctaaaaactt agaaggttta agtatagaac aattaataga gattagagat
    53221 aaagccattg ataaaaattt agaaggtctt agggaatttg gagaagattc agtaaaaaaa
    53281 cgtaaagatt caattttaaa ctttgaattt ggattagtag cttttgttaa caagaattat
    53341 agcaacttaa cagaaaaaga gaaattaata agacaaatta atattgttaa ctatgaaaac
    53401 tctataaatg atataatgga tccttcaggt tcattctttg aattagcaaa tcagaattac
    53461 caagtgaatg agtttctatt aaaaaacgga aaagattata aagaatggtc atatgaaggt
    53521 ctagattttc taattgatca tctagaagaa ctgtgcaaat ttgttgattt tgatgtaaaa
    53581 gatgtaatag atattattga tcctacaata aaaacagacc tttcagagga acaaaagaaa
    53641 tctttgaatg ataaattaaa gaaaatatct tcaagtgata ctttaagtga agaagttaaa
    53701 aaggagataa aaaactggga aaacaattta aataatttaa atatgaataa aaataattct
    53761 aattaatttt ctcataagaa aaatgcttat atcgtgataa tgtgaaatgt acataattat
    53821 aaacaaaaaa ggggctgtga cactaaaaaa tagtgctacg ccctttttta tttgtaataa
    53881 tcatcaatac aaattatttt attatatgta attatttatg tcttaaccaa ttttatcgaa
    53941 aataacagtc ttctctttta aagagatttt tcctacttga tatccatatt catttaattc
    54001 ctttttatat ttcaaaaacg aatggtctat ctctatccca gcaatatctt ttatttcttc
    54061 aaaagttaat ttaaacgatt gactattgtt tttcttaaca tattcccata aagaactata
    54121 tttactcatc ttatatcact ccttttaaaa tcatatctta tctattaatt tatattctat
    54181 cattattaat
//
LOCUS       DAMJYC010000018        53792 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJYC010000018 DAMJYC010000000
VERSION     DAMJYC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.019"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            201..1277
                     /locus_tag="PC472_001149"
     CDS             201..1277
                     /locus_tag="PC472_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2726484.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1380..2222)
                     /locus_tag="PC472_001150"
     CDS             complement(1380..2222)
                     /locus_tag="PC472_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2726485.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2406..2978
                     /locus_tag="PC472_001151"
     CDS             2406..2978
                     /locus_tag="PC472_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2726486.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3141..3413)
                     /locus_tag="PC472_001152"
     CDS             complement(3141..3413)
                     /locus_tag="PC472_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726487.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGNHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3527..4399)
                     /locus_tag="PC472_001153"
     CDS             complement(3527..4399)
                     /locus_tag="PC472_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2726488.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4695..7802)
                     /locus_tag="PC472_001154"
     CDS             complement(4695..7802)
                     /locus_tag="PC472_001154"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2726489.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7895..8127)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8302..8829)
                     /locus_tag="PC472_001155"
     CDS             complement(8302..8829)
                     /locus_tag="PC472_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2726490.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8864..9649)
                     /locus_tag="PC472_001156"
     CDS             complement(8864..9649)
                     /locus_tag="PC472_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2726491.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9659..9916)
                     /locus_tag="PC472_001157"
     CDS             complement(9659..9916)
                     /locus_tag="PC472_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2726492.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9935..10393)
                     /locus_tag="PC472_001158"
     CDS             complement(9935..10393)
                     /locus_tag="PC472_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2726493.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10433..11137)
                     /locus_tag="PC472_001159"
     CDS             complement(10433..11137)
                     /locus_tag="PC472_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2726494.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11147..11782)
                     /locus_tag="PC472_001160"
     CDS             complement(11147..11782)
                     /locus_tag="PC472_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726495.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFKKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12163..13203
                     /locus_tag="PC472_001161"
     CDS             12163..13203
                     /locus_tag="PC472_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2726496.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13666..15021)
                     /locus_tag="PC472_001162"
     CDS             complement(13666..15021)
                     /locus_tag="PC472_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2726497.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15047..15316)
                     /locus_tag="PC472_001163"
     CDS             complement(15047..15316)
                     /locus_tag="PC472_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2726498.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15606..17561)
                     /locus_tag="PC472_001164"
     CDS             complement(15606..17561)
                     /locus_tag="PC472_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726499.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17744..19219)
                     /gene="spoIVA"
                     /locus_tag="PC472_001165"
     CDS             complement(17744..19219)
                     /gene="spoIVA"
                     /locus_tag="PC472_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2726500.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19537..20556)
                     /locus_tag="PC472_001166"
     CDS             complement(19537..20556)
                     /locus_tag="PC472_001166"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2726501.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(20587..21201)
                     /gene="plsY"
                     /locus_tag="PC472_001167"
     CDS             complement(20587..21201)
                     /gene="plsY"
                     /locus_tag="PC472_001167"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2726502.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(21236..22555)
                     /gene="der"
                     /locus_tag="PC472_001168"
     CDS             complement(21236..22555)
                     /gene="der"
                     /locus_tag="PC472_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2726503.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(22589..23917)
                     /locus_tag="PC472_001169"
     CDS             complement(22589..23917)
                     /locus_tag="PC472_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2726504.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEVGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     DALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(24155..24355)
                     /locus_tag="PC472_001170"
     CDS             complement(24155..24355)
                     /locus_tag="PC472_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726505.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(24357..25289)
                     /locus_tag="PC472_001171"
     CDS             complement(24357..25289)
                     /locus_tag="PC472_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2726506.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(25334..26266)
                     /locus_tag="PC472_001172"
     CDS             complement(25334..26266)
                     /locus_tag="PC472_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2726507.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(26321..27610)
                     /locus_tag="PC472_001173"
     CDS             complement(26321..27610)
                     /locus_tag="PC472_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726508.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(27639..28331)
                     /locus_tag="PC472_001174"
     CDS             complement(27639..28331)
                     /locus_tag="PC472_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726509.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(28346..29107)
                     /gene="pgeF"
                     /locus_tag="PC472_001175"
     CDS             complement(28346..29107)
                     /gene="pgeF"
                     /locus_tag="PC472_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2726510.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(29104..29565)
                     /gene="nrdR"
                     /locus_tag="PC472_001176"
     CDS             complement(29104..29565)
                     /gene="nrdR"
                     /locus_tag="PC472_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2726511.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(29587..29850)
                     /locus_tag="PC472_001177"
     CDS             complement(29587..29850)
                     /locus_tag="PC472_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2726512.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(29954..30727)
                     /gene="sigG"
                     /locus_tag="PC472_001178"
     CDS             complement(29954..30727)
                     /gene="sigG"
                     /locus_tag="PC472_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2726513.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(30819..31541)
                     /gene="sigE"
                     /locus_tag="PC472_001179"
     CDS             complement(30819..31541)
                     /gene="sigE"
                     /locus_tag="PC472_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2726514.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(31566..32438)
                     /locus_tag="PC472_001180"
     CDS             complement(31566..32438)
                     /locus_tag="PC472_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2726515.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(32728..34002)
                     /locus_tag="PC472_001181"
     CDS             complement(32728..34002)
                     /locus_tag="PC472_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2726516.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(34286..35443)
                     /gene="ftsZ"
                     /locus_tag="PC472_001182"
     CDS             complement(34286..35443)
                     /gene="ftsZ"
                     /locus_tag="PC472_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2726517.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(35633..35986)
                     /locus_tag="PC472_001183"
     CDS             complement(35633..35986)
                     /locus_tag="PC472_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2726518.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(36085..36759)
                     /locus_tag="PC472_001184"
     CDS             complement(36085..36759)
                     /locus_tag="PC472_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2726519.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(36764..37462)
                     /locus_tag="PC472_001185"
     CDS             complement(36764..37462)
                     /locus_tag="PC472_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2726520.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKAIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(37485..38225)
                     /locus_tag="PC472_001186"
     CDS             complement(37485..38225)
                     /locus_tag="PC472_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2726521.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(38495..39721)
                     /gene="murG"
                     /locus_tag="PC472_001187"
     CDS             complement(38495..39721)
                     /gene="murG"
                     /locus_tag="PC472_001187"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2726522.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(39739..40869)
                     /gene="spoVE"
                     /locus_tag="PC472_001188"
     CDS             complement(39739..40869)
                     /gene="spoVE"
                     /locus_tag="PC472_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2726523.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(40921..42276)
                     /locus_tag="PC472_001189"
     CDS             complement(40921..42276)
                     /locus_tag="PC472_001189"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2726524.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(42295..43263)
                     /locus_tag="PC472_001190"
     CDS             complement(42295..43263)
                     /locus_tag="PC472_001190"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2726525.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(43304..44677)
                     /locus_tag="PC472_001191"
     CDS             complement(43304..44677)
                     /locus_tag="PC472_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2726526.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(44844..46823)
                     /locus_tag="PC472_001192"
     CDS             complement(44844..46823)
                     /locus_tag="PC472_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2726527.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(46835..47182)
                     /locus_tag="PC472_001193"
     CDS             complement(46835..47182)
                     /locus_tag="PC472_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726528.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(47663..48598)
                     /gene="rsmH"
                     /locus_tag="PC472_001194"
     CDS             complement(47663..48598)
                     /gene="rsmH"
                     /locus_tag="PC472_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2726529.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(48628..49374)
                     /locus_tag="PC472_001195"
     CDS             complement(48628..49374)
                     /locus_tag="PC472_001195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2726530.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVVGGIIAHVYLSKKNKNNISEE"
     gene            complement(49768..51054)
                     /locus_tag="PC472_001196"
     CDS             complement(49768..51054)
                     /locus_tag="PC472_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2726531.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(51069..52334)
                     /locus_tag="PC472_001197"
     CDS             complement(51069..52334)
                     /locus_tag="PC472_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2726532.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            52650..53747
                     /gene="ychF"
                     /locus_tag="PC472_001198"
     CDS             52650..53747
                     /gene="ychF"
                     /locus_tag="PC472_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2726533.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        16694 a         9828 c         5698 g        21572 t
ORIGIN      
        1 agacttaatt tattactttt atagaatttt cacaataaaa agtcatttta cttccgtatt
       61 taatgcaaaa gtagaaatta aacttaatat aaaaaatata taacaaattt tttttatatt
      121 tctagaagaa aagtatactc ttttttgttt aataatgcta aaattgattt gtattgaatt
      181 ttgattagga ggaaattatt atgaaagctc aaagattaaa tgctgtactt gaacaaatga
      241 aaaaagatga tatttctcaa atgttagtaa gtgaccctac ttctatattt tatcttacag
      301 gagtactaat ccatccaggc gaaagactat tagcattata cttaaattta aatggcaata
      361 ataaattatt tataaatgaa ttattcccag tttcagaaga tttaggtgtt gaaatggttt
      421 ggtttaatga tactcaaaat ccagtagaaa taataactga acatatagac aaaaatgcta
      481 ctatgggtgt tgataaaaac tggccagcta gattcttatt aaacttaata gaattaggtg
      541 gaggaagtaa gtttgtaaat tcatcttata taatagatac tttaagaatg tgtaaagatg
      601 aagaagaaaa agagttaatg agaatagctt caaaattaaa tgacaaggct atggaacaac
      661 ttaaagctac tgtttctggt gaacttacag aaaaacaact tgttggtaaa ttatctaaaa
      721 tatatgaaga cctaggtact gatggatttt catttgaccc tattataggt tttggaccta
      781 atggagctaa tccacatggt gaacctggaa atgcacttgt gaaaccagga gatgcaataa
      841 tcttagatat aggttgtata aaagataact attgtgctga tatgacaaga actgtattct
      901 ataaagaaat acctgaaaaa ggtagagaaa tatttgaaat agttcttgaa gctaataaga
      961 gagctgaagc tattgtaaaa ccaggtgtta gattctgtga tatagatgct gctgctcgtg
     1021 actacataac tgaaaaggga tatggacagt acttcactca tagaactggt cactctatag
     1081 gacttgaagt tcatgataaa ggagatgtat cttctataaa tacagatact gtacaaccag
     1141 gaatgatatt ctctatagag ccaggtatat atttaccagg tgagttcgga gttagaatag
     1201 aagatttagt acttgttact gaagatggtt gtgagatatt aaacaaacat gacaaagaaa
     1261 tatgtgtagt tggttaattc taaaatcaat ttgataaaat taaaaagcca tataaaaatg
     1321 ttttaaacat ttttatatgg ctttaatttc tcaatctatg atttatttca atccattatt
     1381 tatattgcag ttattaagat tgcacccttt gaagtatatg tccccatcac tgaaccaatt
     1441 tcacagatta caacatcttt gtattttaca tccttttcca taacttccct taccttttga
     1501 gccttttctg ggcaattagc atgtcctata aataaaacct tttcttcttt atctttaatt
     1561 tttgactcta ctaaagtaat cagctttttc aaactgtttc cttcacctct tgccttatct
     1621 ataggagtta caacgccatc tgcaacttgt attatcgcct taaaatttaa ggtatttata
     1681 atttttcctg ctattggatt tattctacca cccttaatag cattttctaa agtttcaagt
     1741 gttccaaaaa atggaggatt atctttatat ttatttatta ttgaaacaat ctcttcaatt
     1801 tcttttcctt cttttattaa tattgctgct tttaacacta attgtcctat tccaattgac
     1861 ccactaagtg aatctataac ctcaactttc ttgttatttc cttccttttc aaacatatct
     1921 tttgctaaaa gtgcactatt ataagtccca gataatcctg aacttatatt aatcactaat
     1981 acactttctt cctgacaatg gtatgcttct aaaaatttat caggagaagg acatgatgtt
     2041 tttggtagta ctttactctc actcattttt ctatagaaag tttcattatc tatttccttt
     2101 ccagatatat aactttcttc tccaaaagat acatttaaac caacaacttc tatgttgtat
     2161 ttttctatta tatcatcagg taagtcacat gcactgtcca ctatcaattt tattttatcc
     2221 atattaacct cactataaga tattatttta tttattcagt cggtcgtacg accgatttaa
     2281 ttattatttt atatattgaa tgaaaaaaag tcaatagata tagattctcg ctttattgac
     2341 aataaatatc cataagctta taatgttatt aatttgttat aaatatacat ggaaaggtag
     2401 gttttatgaa aacaaataat atgaaacagg ctattttatc atctgcaacc aatttaatag
     2461 ccaaaaatgg agttcaaaat actagccttg ctgatattgc aaaagatgta aatataagta
     2521 aaggtacttt gtattatcac tacgcatcta aagatgatat tatatatgat attgcggata
     2581 ctcatttaga agttataact agtgctatac taaactgtgt aaaaaatgtc aaatcaaaaa
     2641 attctcaaat agaaatggtt aacttaatct tagagaaaat atctactata gaaagtcgtg
     2701 gtagggtaca tatgtatctt atttgtgagg caattacttc aaataatgat ttaaaggaaa
     2761 gaataagatt aaaatatatt gaatggagaa ctactcttaa atctgaaatt gttgaatcac
     2821 tagaaaaata taatagtagc gaaaatgaac aggatgctga atcattttct tttttactca
     2881 tgtcaatagt tgatggttta gttgtccaaa gtttattaaa aacagaaaaa ataccttatg
     2941 aaaatatagc ttctttttta gttaatcatt ggatttaaac ttaatactta gataattaat
     3001 aaaagtgaac tatttaagtt atctacttta acagttcact ttcttattta ttctcaataa
     3061 tttttactta aaacattcaa tttttattta aaccttcatg gaatcacaaa ataaaaatct
     3121 cttataaatt taaaatgttt ttatttatgt tcatgtgtat gcccatctga atgttcatga
     3181 ccatgtgtat gtgtatgctc atggctatgc tcatgattac ctgaaaaacc atcatcatga
     3241 ttatggtcgt gacctacacc atttacatgc tcatgttcat gcccatgact gtgagaatgt
     3301 tgatgtgaat gttcatggtt atctcctcca cagcatcctt ctcctccagt cccacaacac
     3361 ccacttgtat gtggattatg atgatttctt actacaaatc tctctcttaa cataattctt
     3421 atacctccta gtatattatt gagtacttca tgtaccccat taactacagt atacatctaa
     3481 ctaggtataa tctcaataca tatggcattt tttaacatat ataatcttaa agaaatttat
     3541 ccttaaaacc tccacctttt acttcttcaa cgttattgac agaaataaaa gcttcactat
     3601 ccatatcata aattaaatct ttaaatttac ctatttcatt tgcagatatt atacaataaa
     3661 tcatcttttt cttattttgt gtatatgcac cttctgcctc caaaaaagtg actcctctac
     3721 ccattttatt cattatcact ttggatattt catcatattt atttgatatt aaaagtatag
     3781 atttttttct atcaaagcaa tcttttccta tatccatagt aaaagaaatt atatacattg
     3841 taattaaagt gtacatcacc aatttagctc caaataaaaa agctcctgca catactacaa
     3901 aaaaattaat aaataaaaat gtatttttca taggtatatc atactttctt ttcattatgg
     3961 ctgctattat atcaagtcct ccagcagtag ctcttgcttt aaaagttaac cccataccta
     4021 taccagttaa tgcaccacca taaatacact gtagcatagt atcatctatt gggaaatatt
     4081 tagcaatatc ttgtgttagc cccagtgcta gagaaaatag taacatattg acaaaactta
     4141 ttagcaaaaa tttattatca aaatactttc tagctataat aaatattggt atgttcatca
     4201 agaaacttga tattccctga tttataccaa ataatttgta taatataaca caaattccag
     4261 ctactcctcc acttaataac ttggctggtg ctagatataa atttattcct aaggttgata
     4321 gaatcatacc caatataata agacaatatt gtaaaaatat gttattatca atacttagct
     4381 ttttactttc accatacatc aaaacgcacc cctttgcata ataaataaac caccctgcag
     4441 aacacacgat aaatttacaa ataaatttat cagaagtagc ttactaaaaa tattattcat
     4501 cctcacttaa acgtttattc attaatattc tatcacatga cataaataat ttatatgatt
     4561 tatatagatt tatttattgt aaattttgga atattaaaat attttattga atagataatt
     4621 atattacata aaaaatccct atagaaatct ataaactaat ttctataggg attttggtta
     4681 actaactatt ctctttattt ttttataact tttatacctg tcatatggtc atttaagttt
     4741 tgttcctcta aagatttatc atctactctg tttatttcta aagctaaagt ttcagacttg
     4801 atatattcat taaaatggtc aacagcctta gcaatttcat catcaccaca gtattctatt
     4861 actatattat ctaatacttc aaagttatta gattttctta attgctgtac tttagatata
     4921 aattctctag cataaccttc attaatcaac tcttcagtta gcttagtatc tagtataaca
     4981 aaaagattgt tttcaacaga tacattaaat ccttccttag cagttatacc tattaatact
     5041 aaatccttat taaattcaaa agcttcacca tctaaatcaa ctgttaaagt ctcacctttt
     5101 tcaagtttat tagcagtttc atgagcattt aatttactta atgctccaac gaataaattc
     5161 atcttagaac ctaataatgg ccctgcttcc ttaaagtttg gttttaaact aaagttcata
     5221 tactcatcta aatccttagc aaatattacc tctttaacat taagctcttc ttttattaaa
     5281 tcaactacat cacttattgt atcttcaaac ttaccatcta ctaacacttt ttgtaatggt
     5341 tgacgaactt ttattctttc tacttctctt gaagctctac ctaaagtaac taagttttta
     5401 gctaaatcca ttttttcttc taatttagta tctactaact cttcattaca tttaggatat
     5461 tcagctaaat gaacagatac ttctcctgtt aagtttctat acatttcttc tgacatatat
     5521 ggtgcaaatg gagctatagc tctacaaaga ccatgtaaca attcataagt tgtattgtac
     5581 actgcttttt tatcttctgt taattcagtt gcccagaatc ttcttcttga acgtcttata
     5641 taccagttag ataaatcatc atttataaaa tcttgtatca ttcttaaagt tttatttaac
     5701 tcaaatatag ctaaattttc ttctacatct ttcattaaat tattgaactt agataatatc
     5761 catctatcaa gttctggtct atctttatat tctacaaaaa attcagttgg atttatatca
     5821 tctgtgtttg catataaagt aaagaagtta tatacattct tcatagtacc tataaattta
     5881 ctttggattt cttttaatcc atccatatca aatttagttg gagtccatgg tggagaaaca
     5941 tatagtaaat accatcttaa tgcatctgct ccatattggt caaataattc cattggattt
     6001 actgtatttc ctcttgattt actcattttc ttaccatctt tatcaagtac aaggtctgga
     6061 actaaaacac tcttatatgg tgcttttcct aatacaaatg ttgatactgc aagtaatgag
     6121 tagaaccaac ctctagtctg gtctatacct tctgatatat agtcagctgg gaacaattgg
     6181 tcaaagtttt ccttattttc aaatggatag tggtgttgag caaatggcat agccccacta
     6241 tcaaaccaac agtctataac ctctgttact cttgtcattg gttttccaca tttttcacat
     6301 tttaagtgta tatcatctac atatggtcta tgaagttcaa ctgtttctgg atttacatct
     6361 tctatagctt tttcagctaa ttcagctctt gaaccaactg aatctgtatg accacattca
     6421 catctccata taggaagtgg agttccccaa tatctagttc ttgatattgc ccagtcattt
     6481 agattttcta accagtttcc aaatctcttc tctcctacaa aacttgggaa ccattttaca
     6541 ccattattat tttctattaa tttatcttta agtttagtca tctctatata ccagcttggt
     6601 tttgcatagt atactaatgg agtttgacat ctccagcagt gtggataatt atgaactact
     6661 ttttctttgc tatataattt attttcagaa gctagccatt ttattatttc aacatctagc
     6721 ccatcttcca ttacaaatct accttcccaa ggagtagatg taaatttacc agtttcatct
     6781 actggttgaa taactggtaa atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat
     6841 gctggtgctg tatgaactat accagttcca tcttcagtag ttacataatc tcctactgta
     6901 ataaaaaatg cttttttgtc aacttctaca aaaggcatta attgttcata ttctaaacct
     6961 tctaggtctt tacctttcat ttcttctaaa acttcataat cttcacctag tactttatca
     7021 gctaaaggtt tagatacata atatacctca tcaccttttt taacttttaa ataatctaca
     7081 tcagcattta ctgttaatga aacgtttgat ggtaaagtcc atggagttgt tgtccatgct
     7141 aagaagtatt catcagcatc ttttttctta aatttagcta taactgtatt agtttttact
     7201 tctttatatc cttgtgcaac ctcatgacta gcaagaccag ttccacatct tgggcaataa
     7261 ggaagtattt tatgtccttc atatatatat ccttctttgt tgaatttgtt tagtatccac
     7321 catacagact ctatatagtt attatctaaa gttatatatg gattatctaa atcaacttca
     7381 taagccattc tctcagtcat tattctccat tgtttttcaa atgagaaaac tgactctcta
     7441 catttttggt taaattcagc tattccatat tcttctattt gttgcttgct agtaagacct
     7501 aactctttct ctacttgtat ttcaactggt aatccatgtg tatcccaacc agcttttctc
     7561 tttacttgat aaccagacat tgttttatat ctacaaactg aatctttaag cgttctagat
     7621 aaaacatggt gtactccagg atttccatta gctgttggag gtccttcata gaatacgaaa
     7681 cttgggtcat ctttcccttt ttctaaagtt ttttcaagta tatttatgtc tttccaataa
     7741 tcaaaaactt gtgcttctgc ttgttttaca gaactgtcta ccaatggctt aaatttcgcc
     7801 atgtaaatca ccctttccta ctaaatatta taattccaaa attttttatt ctttcacata
     7861 aatttaaata ctaaaaaact cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt
     7921 accaccctaa ttctatatgt aaactaaata ctagctacat ataacactct aatagtataa
     7981 cggtactaac cggcacagct tagtagaatt taatcgttca gcctgcagct caagagtgat
     8041 ttttatcttt ataaattcat tggttctcag catacccaat tctctgtaaa attatctaaa
     8101 gataaatgtc tctatcaacg cttttaaaaa tttatctgtg tttatatgaa attctataac
     8161 aaataatttg aatagtcaat atatttttga aaaatataat aatttctatt tttcttacat
     8221 ttttatccta tttttaatct ttgttataga tttctcaaac tcacacaatt attctacata
     8281 aaaaataaac aaattacaag cttattctaa agttgtagca gcttcatcat tactataatt
     8341 atacactgtt gttccttcaa atgcgtttac acatttttca tctacatcat aaaaaatctc
     8401 atcaacactt cttatttcat cttctaacag tgatttaaat ttatttctaa atatcttaaa
     8461 ttctttgact aaagcatcat actcttttct tatctctata acacggttat tagcttgttc
     8521 tataatttgt ctagctttta aatcagcttc ttcaacaatt atcttagcct ttttattagc
     8581 cgcgctacaa gtgtcttctg ctgcactttg agcagttatt aaagtagctt ttagtgtttc
     8641 ttcaatattt tcatacttgt ttacttggtc ttgatataac cctaattttt ctttaagagc
     8701 tgtattttcc ctacatagag attcataatc ttccttgatg atatctaaaa attcatcaac
     8761 ctcttcatct ctataccctc ttaaaccttt tttaaactct ttattttcta tctcaattgg
     8821 agttagcatt acatttcctc ccagctataa tttaaattag tccttataca attaatcttg
     8881 cttgaacttt tatttttcct tttttagtaa tatctcctat ttgtgttacc ttagctcttc
     8941 ctttgcctct aatagaaatc aaatcatcat tttttactat tttagaagtt gaagttatct
     9001 tttcataatt gacatggact ttttctccat ttatgtattt tgcactatct tgtctagata
     9061 tattgtaaat accacttatc acacaatcta acctatctga agatactgta aatgatactt
     9121 ctttatattt tgaagaacta ttaacaatat tttctctgga aatttccttt acaattacat
     9181 tatttcttga aaccttttct aagtttacaa ttataaaatc gcaaatatca ctacttacaa
     9241 ctacttgaca aaagctatca tgtataatta tatctccaat tttttctctt tttataccta
     9301 gacccagtat tgaccctaag taatctttat gagatatatt tttaaatttg aaattccctt
     9361 ctatttgtaa aaatctaaga gtatcttcta tatcttcata ttccatgtaa aatggataaa
     9421 taaatacagt gcttctttca gcttgttcat aacctccatc cacactgtat tttatttcat
     9481 ttgtagaatt taatattgct acagcatttt tcacttcata tggattaaga aactccgtag
     9541 acttaacatc atagtttttt atacaactat tagccttgtc tatcacttta aacattttat
     9601 ttttcaagtc tatatccttt atgtgatttg ttagttttaa tttatccatc ctatcacctt
     9661 agaatataac caatactaat tgtcttagga acatcaataa tactattgca attaaaggag
     9721 aaaaatctat aggtccattt gtatatttat acattataga ccttattggc ccttctattg
     9781 gctctgtaag attttccaaa atatcaaaaa ttttactttg tcttccattt ggaaaccaag
     9841 tcatcaagct ttttataact atcatccatg ctattatatc taaaagataa taaagtgcaa
     9901 ttcttattgt tcccatatat taaccgcctt tttactattt attttgccaa gggaagaatg
     9961 ctttactttc taattctttt ttcatatttg catctatatc cacattatta ggagctagta
    10021 taaatattga tttagaaacc ttttgtatac ttccatctaa gacataaact gccccattca
    10081 taaagttaaa tatagattta cgaacttcag aattagtcaa tccttccaaa ttcactataa
    10141 ctgcccttct ttgttttaga tggtctgcta tagttgcagc ttcatcataa accttaggtt
    10201 caactattac aactttcatt tggctagttg tatgtaaatt tacaatttta tttgcttttg
    10261 cagtactaaa gccactgttc atttcttctt cttgaactat atcgtccatg ccactttcat
    10321 attcatcttc aatgtaatct tcttcttcat caactatcca attcttaaat ttagatatta
    10381 ttccatttga cattattaac ttcctcctta aaatttattc aaatttgagt aattattttt
    10441 tattgtagtt tctttctcca aagattgatg ttccaactct aacaatggta gccccttcct
    10501 ctatagcaat ttcataatcg tgactcattc ccattgacaa aacatccatc cctacattag
    10561 ggatgtttag ttcttttatt tgttttgata aatctttaag acctttaaat acacctctta
    10621 tctcatcttc atcttctata aaaggtgcca ttgtcattag accctttatt tttatatttt
    10681 tatatttttc tgacacttct ttgacaaagt ctacaacttc ttctctatca agtccatgct
    10741 tagactcttc tttagatata ttcacttgta ctaagcaatc tatttctctg tttatttgtt
    10801 ctgcacgttt ttgaatttct tcgcataatg caattctatc taaagagtgt atcatacaaa
    10861 ctttatcgat tatgtactta actttattgg tttgcaaagt tcctatcagg tgccagttta
    10921 atttatcacc tataatatca tactttctag ctaattcttg gggtttattt tcaccaacag
    10981 aagtgattcc ttccctcatt gcctcattaa ctaagtctac atcaacagtc tttgtgactg
    11041 ccaaaagggt aatctctttg ctgtctctat ctactttttt tgcagcttta tcaattttgt
    11101 ttcttatctc agacaaatta tctttaatag aacccattat tatcacctac tttatcctta
    11161 tatttgtgtt tatactgtta ggtcttaaaa ttattttgtc atatagatta acacttgtaa
    11221 ctccatttat gtcattttta taataatcca caaatacaga agattcattc tcataagtta
    11281 ttccttctaa tatgacaaat tttacacttt gtgattgttc atcaattaca tatacaccac
    11341 gtttattgtc ttttgttttt atagaagttt taggtatttt taaacattcc atctgattat
    11401 atattatatc aaattcttcg acacttgtat cctgatttgc aacaatttgg ttacttattt
    11461 ttaattttac cattatttta ttattatttt catatatttt gtaaactttt gcatctaatt
    11521 tttgctcatg atattcaatt attacatttt gatttttttt aaagttatta gaatcttctt
    11581 tactgatgta tgtagataga taattactct ggtcattggc atctttttct atagttcctt
    11641 ttacatgaat ctttgcatca actttttcat tttttaacac caacgtatct acatttttgc
    11701 taattatcat tactaatata ttaaatacta aatatataag aataccaagt atcactaatc
    11761 tggtatattt tacctttttc aaagactctc cccctataaa attactttca ttataagtac
    11821 ttcttctaaa aaatgtttat tcctttttta taagctaata ttttacgttc taataattat
    11881 tttttccata ttaaatttat tttttatcaa tcttcctata caagactgca ttatttatat
    11941 ataattgtat tataaagtat aagtttaatt tacattttat acgttaagct tactttatat
    12001 gtttttatag ttatttttat ttttagatgc aatataaaac caattaagat ttaaaataac
    12061 ttttgttatg ttgacattta tttaggtaat catttatgaa ttaggataac gttatcataa
    12121 caaaagttta tgtcctaaaa aaaatacaag gaggattttc ttatgtcaga aattttatta
    12181 ggaacaggtt tacttttatt ggttctatgt tgtttcacaa tttttagtta caaggcccca
    12241 tatggtatga aggctatggg agctctagca aacgctgcat gtgctacttt cttagtagag
    12301 gcattcaacc tttctttact tggtgatgtt ttaggtatgg aattttttag aagtgttgga
    12361 gctgctaatg gtagtcttgg tggagttgct gctgctatac ttgtgccttt agccttaggt
    12421 gtatctccag tttatgcagt tttaatagga ttatcttgtt tagatttttc tatattatct
    12481 ggattcgttg ctggatactt tgtttctttc ttaattaagt ttatagagaa aaaagtgcca
    12541 cctggagtgg atttagtggt tgtaattctt ctatgtgcac cactatctcg tcttatagca
    12601 tcaaatgtag acccattagt taatagtaca ctactacaaa ttggacaagt tttgatagaa
    12661 gctaccaaca ttagccctat aataatggga attatactag gaggtctaat aacagtagtt
    12721 ggaacgtctc cacttagttc aatggctctt acatctataa ttggtcttac tggtcttcct
    12781 atggcaatag gagcattagg tgcttttggt tcagctttta tgaactcaac actatttgca
    12841 aaattgaaat taggttctaa aaaagatatt atatctgttg ctattgaacc attaacccaa
    12901 tcagacataa tttcagctaa tccaatacct atatattcta ctaatttctt tggaggagcc
    12961 ttttctggta tcattgtagc cttgatgggt cttgtaaaca tgacaccagg tacagctaca
    13021 cctgtggctg gttttgctat aatgtttgct tataatccag ttcaacaagt tcttataaca
    13081 gctggtttat gtgctttatt caatgtaatt gcaggatttt taggttcaaa aatattcagt
    13141 acctttaaag ttcttactgc tggtgaaata cgtggtactg atgtagaaga agaaattgct
    13201 taaaaaacaa taagatttat aaatttatat ctcaagtagt agagttttat attgtaggca
    13261 attttattaa ttgcaaacgt tttatttttt atttatgttt atgattatta tgtgtgttga
    13321 atatttagat aaatttttat ttattatttt tatttagatt tttgtttagt tttttatttg
    13381 gttgatatat taggaataaa aattagtata gtttattact agttagatag cataactttt
    13441 tagatacata aaaaatttta tattaattag atattattaa aataaaagcg atacatgaat
    13501 ttttaaatgt ccatgtatcg ctttattttt gccataatct taacaactaa tttttaaagt
    13561 ttattatctt aacaaagttc attatcttat ataatattta aatgcataga cttctttaat
    13621 ttaaccatct aaatatttaa ttaaatatta ttatatgtaa tataactaat ttttaaatga
    13681 atgtatagga gctggaatcc ttcctcctct ttgagtaaat aattcagatg aatttgtatt
    13741 tataggcatt actggtgctc gtcctaaaag tcctccaaat tctaccatat cacctacatc
    13801 acatcctggg gctggtataa ttctaacggc tgtagtctta ttattaatca tacctattgc
    13861 tgcctcatca gctatcatgg ctccaagcgt acctgctgta gtatttcctg gcacagctat
    13921 catatcaaga cctacagaac aaatagcagt cattgcttca agcttatcta tacttaaagc
    13981 acctttaata acagcatcaa tcatacctgc atcttcagat actggtataa acgctccact
    14041 aagtcctcca acatgtgaac atgccattac tccacctttt ttaacagcat catttaaaag
    14101 tgctaatgct gctgtagtcc catgagtacc aacaacttca agacccattt cctctaatat
    14161 atttgctact gaatcaccaa tagcaggtgt aggagctagt gataagtcta ttatcccaaa
    14221 tggaacatcc aatctactag aagcttcctt tgccacaagt tgtccaactc ttgtaatttt
    14281 aaatgcagtc tttttaactg tttctgaaac aacatcaaaa ggctctccct ttactttctc
    14341 taaagctctc ttaacaacac cagggccact tacaccaaca tttataattc tatcagcctc
    14401 accaacacca tggaaagctc ctgccataaa tggattatct tctactgcat tacaaaatac
    14461 tactaatttt gcacaaccaa aaccttttgc atcttttgtt aattctgcag tttcttttat
    14521 gatttctccc atttctttca ctgcatccat atttatacca catcttgtag aacctacatt
    14581 tactgaagca catactttat tagtacttgc tagagctttt ggaattgact taattagtat
    14641 cttatcacct tttgtatatc ccttttgaac taatgctgag aatccaccaa taaaatctat
    14701 acctaaatcc tctgcagcct tatccaatgt ctgtgcaaac tttacatagt cattttcatc
    14761 tgttgcacct gcaataatgg aaattggagt tactgatacc cttttattta caattgggat
    14821 accaaattca cgctctatat cttttccaac cttcaccaaa tctttagcag aactcataat
    14881 cttattgtat attttttctc tagctttatc tccatcaggg tctatacaat ctaataaaga
    14941 aatccccata gttatagttc ttatatccaa ctgttcttca tctatcatct ttattgtttc
    15001 aagtatattt cctacattca ctattatcac ctccccttac tgtctactac agattatgca
    15061 ttgaattaaa tatttcttct cgctgaattt ttacatccac tgataatttc ttacctcttt
    15121 caaccaagtc ctcaaccgtt ttctcaaaag aatttaatga tttctctaaa tcaactatca
    15181 taatcatagt aaaataatcc tgtaatattt tttgtgatat atctactatg ttgatatttt
    15241 gcttgtataa ttcatcacta attcctgcca ctatacctgg tttatccttt ccaattactg
    15301 ttaaaattgc tttcataata atatcctccc ttattttttt ataaatctta tatttttaca
    15361 aattgaacta tgtgttaaca tatatttcat ttatttgtta gtaaaccact ttttatgtat
    15421 atatttaata tattttaata cctatacatt catttgtcaa ttttttttca atattctatt
    15481 atttttatac tatttagaaa attcatatat tatatatgtt ttctcaacaa caaatacagt
    15541 tttcataata atgaaaggta ttacttagca atctcatgat tttctaaata atacctttct
    15601 aaaaattatt tttttaattt tatgaattta attatacttt ttctagtatc aggatttaca
    15661 attaatatag aaccaatcat aattggtgtt agtatacaaa ataaaaaaat aatatctttt
    15721 tttagattat attctttaga ttctaattta taattattat ttattattcc atcataatca
    15781 cttatatttg acttattttt atttgtatct tgatttttgt tagaaatact ttccctataa
    15841 ggaaaagaat gttcattggt accaaatact gattttgagc ttaaatcata attatcatca
    15901 ttttctgtaa ttgaattttt attgtaaaat cctttttctt tatcagaatt attttcttta
    15961 ttaccgtctt taggtatatc atgaggattt ttattttcat tgctatcagt acttgattct
    16021 acatcatctt taggagtatt tggttgtttc tcatcagaat tatcattttc attattatta
    16081 ttttcattat tattgttttg attgttatta ctatcattat tttcatttcc attattccca
    16141 tcttgattgt tatcatcctc atctctatcc gggtcgtctc tacctcctcc tccacggtca
    16201 ccatcaatat catcattttc ccgttcatca tctggcgtct cttggttgtc tccacctccc
    16261 attataaatc ctggtttaaa aactatattt gaataccctt cttttaatcc tcctacaact
    16321 tttactctaa aacaacgcaa cttaaaatct tcaaaaaaaa gtatagcttg atctctaata
    16381 tcctcctgct cttcagatat ccaattgatt ccatcgcttg aatattcaac aaaaacctta
    16441 cttgcactat acggcatatc ataaaataaa acagctacat atccattttt tgtattttga
    16501 aattccagtt ccaaattgtc tattggtttt ttctctataa cagatacatt caaaatagga
    16561 actacatcat catttattat aattttttct ttatttaaca tttctgtttt aaactttcct
    16621 gttatattaa aatcttctcc cttttccaac ttttcataat aatcagtaaa atcccattct
    16681 acatctatat caagtcgatt accactagat gtttctaata ccatatattt gggaaatttt
    16741 atattttcta ttttatcatc tttacttact tttatttcat cactaaatac accaattccc
    16801 ctatgacaac atttcacaat attatagcca gaatcttcag ctatagaggg tataagctca
    16861 ttataatcat caattatata aactttctta ctttctgata taattgattg tgccaactta
    16921 ttattatctc ttagtaaccc actttcattg ttgctactat atgcttttat tgaagtttct
    16981 tctatctcaa catcaccttt agaataaaca cctatagcat gttctccatt cacttcaata
    17041 tccttattta gtattctaat atcattttca gtataaattc ctattgcatc ttttccattt
    17101 gcctttattt tttcaacaga ttcgtaggat gatttaatat gtaagtctcc tccttcttca
    17161 acgtataagg ctgtcccatc agtagcaatt atgttgttta taccaatact tatacttcca
    17221 ccattttcta tatgtattac tccttctttt cctccttcac ctattatatt caattcatta
    17281 tcatcaataa taaatctccc atgatttttt atcaatattt tatatttgtt ggtatctatt
    17341 gtaaaattac tgtcataagg aatataaaat ctaaactctt catctattac catatcacaa
    17401 gaaagagtat aaactatttc ttctttatta tttttatttt cttgaaccca cctttcaaaa
    17461 tcttcaagac aattttcctc ttgtccaaaa actttatgtg gaaaacttat aaaacacaaa
    17521 actattatac tcataaaaat aaatatctca tactttttca tcttacactc ctagaaattt
    17581 ttagctttga aacaatcctg tcgaaacata tacttcaccc actataattt ttccacaatt
    17641 atcaattatt gtcaatttat ataagtatct ttattaattg ttataaataa aaagctactt
    17701 actaaagcaa acagacgttt cactttaata agtagctata tttttataac aaaatagtta
    17761 taatattaga acttccttca tttacaattt tttgtaaagt cttttgcatt ttaactctta
    17821 tctcttctgg catagtatat aatttacttt gtaattgctc ttttactaag tcatgtagtg
    17881 atttaccaaa catatttgat tcccataaat ctgctggttg ctcttcaaat acttccataa
    17941 gatattttat catctcttca ccttgatttt gatttccaac tattggagaa acctctgtag
    18001 atatatctgc ttttataata tgaagtgatg gtgcattggc ttttaacttg ataccatatt
    18061 gttttccttg tttcattatt tctggttctt ccagactaag ttcttctaag gaaggagcta
    18121 ctactccata tccttttatc ttggcatcta ttaaagcact ttctatttta tcatactcat
    18181 ttttaacttt agatagtcta gttatcagac taagcagttg ataatctcct tcaattttaa
    18241 atccactttt ttcttcaagg acattataga ataaatcttg ctttgtctgt aaatcaatat
    18301 ttataacacc ttctcctagc tctacattat caacacctgt atcttccaaa aactctaact
    18361 cagaaaatcc ttgtataata ccttctatat cccttatttt acctatatct ataatacttt
    18421 gttttaatgt tgttattatg ctacttttta tccaatgatt tctttcaaga ccttcaaccc
    18481 attttggtaa gttgattctt atttctgtaa gtggaaaatc atataataca gcttccataa
    18541 cctcctctat atcttcttct tccatttcaa ccacattcat tggaagaact ggaacttcat
    18601 acttttcttc caattcactt ctaagcatac tcgtttcctc actttttgga cttaacgtat
    18661 ttaaaaccac tgcaaatggt tttttaagat ttttcaattc ttgtataact ctctcttctg
    18721 gttctacata gctttttctg tcaattccag taactgaacc atctgtcaag acaactatcc
    18781 ctattgtaga atggtctttt ataacttttt tagttcctat ctctgctgcc ttttcaaatg
    18841 tcatagcttc ttgtgaccaa ggagtggata ctaatctttg ttttccacct tcctcatgcc
    18901 ctaaagctcc ttcaactatg tatccaacac aatctaccat tcttactttt aaagatactg
    18961 tgtcttttat ttttatttct acaccatctg ctggtacaaa ttttggttct actgtcatta
    19021 tcgttttacc tgaaccactt tggggtatct catctcttgt tctatccttt ttaaattcat
    19081 tgtctatatt aggtataacc aacttttcca taaattttct tataaaagtt gattttcctg
    19141 ttcttacagg tccaactact ccaatatata tatccccctg agtccttttg gatatatctt
    19201 cgtatatgtt attattcata ttattcctcc tatacatcta atcttattaa attatattta
    19261 gagttaaggg ttaatattct taaaatagaa aaataaagac tagcaatatt aatttcccct
    19321 atatacaaaa actagtaaac tacaatgcta attgaaatta ttaaaatcca ttacattaaa
    19381 agacataatt atagtcatga taattacaaa ttatatataa aataatttat tatatcgtat
    19441 atacatagac tatgctaagt aaaattaaaa tagccctctt tatattaaag agggctaaaa
    19501 caaatctatt atttttttat tttattatac aatagtttat atatcatcac ctaaaactac
    19561 ttcttccatt tcatgctttt tacttctcat cattagttct attccaactt ctttaggatt
    19621 tgaacctttc tttattacag aatatatagc attagttata ggcatatcta tatctaattt
    19681 ttcagcaaca tcatgtgcta cttcagtagc agttatgcct tctacaacca tctttacttc
    19741 ttttaaagta tcttctaaac tcatcccttt acctatgagt ataccagctc ttctatttct
    19801 actatgcata cttgtacaag taactattaa atctccaata cctgaaagac ctgcaaaggt
    19861 agacatatta gctcccatag caattccaag tctgctcatt tcactaatac ctcttgtcat
    19921 aagggcagct tttgcattat caccataccc taatccgtca catatcccag ctccaaatgc
    19981 tataatattt tttaatgctc ctccaagttc tactccaact atgtctggat tcgtataaac
    20041 tctaagttta gggctcataa ataaatcttg tatcatctgt gctattttta aatctttaga
    20101 agcaactact acggtagtag gaatatccct tgctacttct tcagcatgag atggcccaga
    20161 tagtattaca tatggatttt gtggtaattc ttcttcacag acttgtgata atctaagccc
    20221 agtccctttt tcaagacctt tagctacatt aacaataact tgtccttctt ttataaatgg
    20281 ttttattttt tggcaagtac ttcttacagc ctgtgatgga actgctaaaa ctattacctt
    20341 acaatctttt atagtttttt ctatatcagt acttatagtt atattatttg gaaataatac
    20401 tcctggtaaa tagtctgtat tttctttagt tctgtttatt tttttagctt gttcctcttt
    20461 tcgagtccac atgctcacat cattaccttt ttttgcaagc cctaaagcta atgcacttcc
    20521 ccagcttcca gtacctaata cacatacttt ctccatgttt aacaccacct gagtataata
    20581 tatttactat tctttctttt gaccaatttt tctctctgtc ccatttaata atcgctttat
    20641 atttgctctg tgattatata ttactgatgc tgttaagaac agagctacta ttaaaccagc
    20701 tttatttttt accataatca taaatattgg tgaacatcct attcctacaa cagaacccaa
    20761 tgaaacatac tttgttatag ctaccaccaa tataaatact gctagacaca ttagtgttat
    20821 tactggattt acagctagca ttgcccctag tgaagttgca acacctttgc ctcctctaaa
    20881 tcctaaaacc gctggccaat tatgacctgc tacaacacat ataactgcca gatatccagc
    20941 taaaagagta tctattccaa ccaatcttgc tgcaaattct gatattaaaa cagccattac
    21001 acctttaaga acatcaccta aaaatgtcat tgctcctgct ctttttccaa gtgtcctaag
    21061 tacatttgtc gaacctgcat taccagaacc ttgagtcctt atatcaaccc cagcaattcg
    21121 ctttgcaact atgtatgagg tagatatatt tcctaaaaga tatgcaacta cagcaataat
    21181 tatataacta aatatctcca ctgaatttcc cccctttagt tatattttaa gatatttatt
    21241 ttttattttt ttgtctatac tcaaacttta tggaagttcc ttcaaatcca aaattttctc
    21301 ttatcttatt ctcaaggtat ctttgatatg agaagtgagt taaatcctta tcatttataa
    21361 acaatgtaat tttaggaggt ctaatgtcag tttgagtccc atagtaaatt tttaatcttc
    21421 tacctttgtc agatggtggt tgatttaaca ttacagcttc tccaattact tcattcagtg
    21481 ctgaagttga tattctctta gaatgttcat ttgacacata ttcaactgta tctagtatct
    21541 tgttcattct ttgattagtt tttgcagaaa caaatacgat tggagcatac atcataaatg
    21601 gaaatttttc ttttatatct tttgtgtaat tactcatagt cttattatct ttctctatta
    21661 aatcccattt attgattaca aatatgcatc cttttccttc atcatgtgct atacctgcaa
    21721 cctttgtatc ttgttctgtt acaccttcta aggcatctat tactatcaat actacatctg
    21781 ctctatctac agcactcata gctcttatta cactgtattt ttctattgtc tcatatattt
    21841 tactttttct tctaagccct gctgtatcta ttagaagaaa tttctgtcca tttttttcaa
    21901 aataagtatc aattgcgtct ctagtagttc ctgcaattgg acttacaatt actctttctt
    21961 ctccaagtat cttattgagt atagaacttt ttcctgcatt tggtttacct gttatagcta
    22021 ctcttattat atcttcttca tactctgtat ctaatcctgc tggaaaattt tcaactatct
    22081 catctaataa atctccaaat cccatactat tggctcctga aactgcaaaa ggcgttccaa
    22141 atcccaactc ataaaaatcg tatatattat caaattgact ttgactatca atcttattaa
    22201 ccactaagat tactggtttt tttgtctttc taagcaattg tgcaatttct ttatcagcag
    22261 ctgttattcc agctttacca tcaactataa aaagtattac atgtgacatg tccattgcaa
    22321 gcatggcttg atttctcatt tgagataata ttatatcttc gctatcaggt tcaattcctc
    22381 ctgtatccac taatgtaaaa tatttatcta accattctac ttcagcaaat attctatctc
    22441 ttgtaactcc aggtgtattt tcaactattg atatcctttt tcctgcaaat ttattaaata
    22501 ttgtagactt accaacgttt ggtctcccaa ctacagctac aactggccta cttatactca
    22561 ttttatttct ccttccatct tttgttttct attttaaaat tttatccaca aaatcttttc
    22621 cttcattcaa acaagggata atttttattc tcataagttc ttctaattga tttaattcaa
    22681 tattatctaa gaaaatttct tcatctgctt ttagcatact tcttgttata taaagagcgt
    22741 ctcctaattc tttatcttca agttgttctt ttaaatcctt agctgttaaa agacctgata
    22801 cagttattgt ctctccaaaa aacttgtttt tgatttcata tacatttact tgaagatttt
    22861 taaacttgtc catcatagca tctgccatac tctgtataaa ttcatatgca gaatgaccag
    22921 tagctattga tactttcttc tctcttttta gaatcttctc aggcaatata tttagatact
    22981 cttcaatctc tgtttttaac ttacgtatca tacctacacc atcttcaaac tgtaaaaaac
    23041 cttcatattc ttcatatcca ggcaattcac tattagatag tatataaaat tcatctgata
    23101 aaaatgcaaa tcgagtacct aatttttcaa gatatttctg ttgtatttga tgtatttgct
    23161 ctatagtttt acttgcgctc ttatcattaa atatatttaa ttctactaga ttttctctat
    23221 gctttgtaat cccaactgga actatcgcaa cactgtttac atacggataa agttgagcta
    23281 gttcttttat tgttctatcc aattcctttc catcatttat acctggacat aaaacaattt
    23341 gacagttcat ttctatatga gcatctgcca atctcttcat tataccatat aatttccctg
    23401 caaatttatt gcttatcatt ttttgtctta actctggatt agttgtatgc acagatatat
    23461 ttataggact tattctatat tttataatat tatttatatc ttcttcactc atatttgtaa
    23521 gtgtgacaaa atttccttgt aaaaaagata atctcgaatc atcatcttta aaataaagag
    23581 tttctctcat accttctggc aattggtcta taaaacaaaa catacactta tttctacagc
    23641 ttttagcttg gtcaattatc ggatttgtaa attcaacacc caaatcttca tcatagtctt
    23701 tttctatctc gtatatgtag acttcgccat cttttttttg tatttctaca tctaaatatt
    23761 catcacttag taagaatcta tactctatta tatcatgtat tggctgttca ttcacagata
    23821 taagtaaatc tcctacctct atacccactt cttctgcaat actatcttta taaaccttac
    23881 ttataatatt atttactttt tttacattta cttccaatat tttcaccact cttttgtatt
    23941 catcaatgct tattataatg tattttaata tgcaatttat ttatttatta tattttatga
    24001 tagcattttt aagcatttca tgtcataata acacatttta attctaatag tcaatataag
    24061 taaatagcta taaaatagat tagaacaaat aatattcaaa tttaagtttc ttaaaaattt
    24121 aaatattatt tgttcttaaa aaaattttat aaatttaatg ttttactatg attaacgtcc
    24181 catttgtcat atcatgaacc attccaccta cacactttga catcaacaat gccttatttt
    24241 gtaagtcttt ctcatcattt gcataaaata ttggacaacc tccaccattt ttcatattct
    24301 tatcaagtgt aactatagct agggtatatt catttaaacc tatatccatt cccatcttat
    24361 gcacttcctt tctttcctat tttataattt tttagtgata agttttttcc tttagagctt
    24421 gaaagtattg ggcatgactt tacagcttcc attacttttt ccacattctt atctactggt
    24481 aaataagcaa tcattatact ttcatcctct ttatttcttc taggtaatgg tacgaatgaa
    24541 ggttctccat aatctccata taaacctaac cttgaatata tattgtatac tattgcttgt
    24601 ctttgaccgg agtcaaataa tattcctgca ttagcataat ccttattttt aggaattatt
    24661 tgtattccta cacctctatt aatatatact tctctatcgc tttttagacc tatatttgtt
    24721 atacctttta aatctcccac ctttagtata gattcgtcca caaaactaat ttcagcaggc
    24781 attacatcag ctatgtctcc aatacttttt ctagtaagaa gtttttttaa tataaaggct
    24841 aacactaatc cacaaattga acttattacc aaactcatcc cttcacttat tttcgtttct
    24901 gcaagtatta cataatacaa tcctattgtt aaaaaagatg taactataca catataattt
    24961 cttacttcat aggttctagc tatctcttct atgaatgcag ctcctctctt tacaagttga
    25021 acttcctcta aattttgcaa tgtatttctt ctatttgctc tcacttgtct aaattgttct
    25081 gctgctagag ataaaaatgt tatggatgta taagaacgat ttataagagc tggaaccaat
    25141 aatgctccta atgaagaagc gacgaatgct aatataagtt gagatagtaa aacatttggt
    25201 tgcgttggat actgtttttg gtctaaattc aacattataa ttctagatgt tataccaata
    25261 ataacagcaa caaaaaatac tctatccatt aaatcactcc taaaatattt aatttgaaat
    25321 tttacatgta aatctacaaa gtctttctag aaaaatcact ctgctttcct tttgatgttt
    25381 ctatgatagg tgtacttttc actacatcta tcatgacatc tatatctttg agtattggca
    25441 tatatggtat aaccacagta cttttttcta aattggtctt agatatagca acaatatcaa
    25501 cctcatctac atctctatct atccccatat gaataaatag attatgtatt atagcttgtc
    25561 tttgccctat atcatttact attccaaatg cacctaaatc tcttggtata acttctatag
    25621 ccagacattc atttctatac ttttctcttg tactttctag acctatgtta gttattatta
    25681 caccatttac cattaaaata ggcccttcaa aacttatttt tgcaggaact atatctgcta
    25741 tatctgctat actgttcctt cttaaaaatc ttctaaaaat caaaccaaca atagctcctg
    25801 acacaatagc taagattgtg caataaaaaa agctaaggcc atactttctt gcaaaaacga
    25861 tataaactat cgaagatacc agtgcagaaa aaagacttat ataacttcta gattcatacg
    25921 tagatgctat ttcttcaacg taagcatctc cttttggtac taattcttca ttatcgatat
    25981 tctttagtgt tattctttct tgttctgcaa gaccttgaaa ctgttgtatt gctactgcaa
    26041 aaaatgtcaa ggctgcaaac tccttatcta taagtgcagg taatgctata gcacctaacg
    26101 aagctgaaag cccagatata attatctgtt ctaaataatc ttgtggtctt gatgggtatt
    26161 gtttgtctgt aactcttaaa actaatcctc tacataatat tcctacaact aatgcaacta
    26221 taaaagagtg tctaaataaa tttgcattca ataaactttc ttccattttc tcagctcctt
    26281 ctcttatata gtttgtgcaa aaaacaaaaa aaattccctt ctatttaagg aaattttttt
    26341 gtaatattgt aactataaat tcgctaccaa tgccaagttc actttttact tttatacatc
    26401 catttagtga tttgactata tgctttgtta tagccaatcc taaacccgtt cctcctacat
    26461 ccctacttct tgctttatct accctataaa atcgttcaaa tatgcgttct acatcttctt
    26521 ttggtatccc tacaccattg tctataactt taattacgat tttttcttca tcttttaaca
    26581 ctacaacttg tacaatccca ttatcttggg tatactttat agcattatca actaggttta
    26641 agaacacttg ctttatatag tctttattgg atagtatact tattgattta tcatcacatt
    26701 tatataaaac ttctatattt ttttcactag ctgaatgttg agttatttca tatacctctt
    26761 taaatacatc atatactaca acattttcca taagaagagt atctttattt tctacaaatg
    26821 ataacagcaa tatatcatca atcaaccttt ttaatctatc tgattcgctt tctattattt
    26881 ctaagaatct atttctagta ttcacatcta tattttcatt tgactttagt gtttcaacaa
    26941 acccacttat agatgtaagt ggtgttttaa gttcatgact tacatttgca acaaaatcac
    27001 ttctcatatt ttcaagttta acttttttag taatatcttc tatatttact atcgaaccta
    27061 ttattacgtt gttagtattc tgaagataaa ctgggtctac ttttatctta tatacgatat
    27121 catctttagt agtaatatta gcagtttcat tatctttaga accaataaat tgaagtattt
    27181 gttttaatat ttcttcttct tttattacaa cattaatatt atgaccttca actaattgat
    27241 ttggttcact ctttatcatt tcttttgctt catcatttat cagaagtata tttccattta
    27301 catctatagc taaaattcca tgagatatac tctttaatat agaccttaac tgtaaatgtt
    27361 tatactgtac ttcttctaag gttttatcca tattctttat catatgatta aaatttctag
    27421 ctaattcgcc aagttctcct ttggctgata tgttaagcct agcatgatat tcattattac
    27481 ttactttttt tgagacgtat ataaactctt ttaaatattt tcttaatgtt attgtgtatc
    27541 ttacagctaa tataacaatg gctatggaca acataacaat aaaaaaatat atactattta
    27601 aatcttccac aagataacac tcctaaaata taaccttttt aatctatttt gtatcctaca
    27661 cctcttatag tctctatata tttttccgat ttatcttcat cttctatttt ttttcttaaa
    27721 tatcttatat gaacatctac tgttctagtt tctccatagt attcataccc ccatatttta
    27781 tccaaaagat aatttcttga aagtactttt cctttatttt gaattaataa ttttaacaat
    27841 tcaaattcct taagagttaa ctctatcttt tcagagcctt tggtaacttc atgttttgat
    27901 aaatctagtt ttaaattacc tgtagtaagt atattgtttt cttcacctaa gctagtcaaa
    27961 ttatatctcc ttaaaacaga actaatccta gctaacaact cttttatact aaatggctta
    28021 gttatatagt catcagcacc tatttcaagc ccttcaacct tgtcattttc catattttta
    28081 gcagtaagca ttataacagg aatatttttt aagtccttat cacttcttat ttttctcaaa
    28141 acttctattc cacttatatt tggtaacatc caatctaaaa gtattaaatc tggtttaatt
    28201 tccttagcct ttataaatcc gtcaaatcca tcatatgaat aactaacttc gtaatttgac
    28261 acctccaaat tgaattttaa tagctcaact atatgcattt catcgtctat aacaagcact
    28321 ttagtattca ttaaaaagct cctctctatt ttatctgtag taccgttcct attcgttctg
    28381 atgccttatt atgttttcta taagagtgga atttatctgc tctacaacta gtgcataaat
    28441 ttaaatttat tatattttct tttttaactc cactacatct aagcatatac tcattaattt
    28501 tccataaatc taaatagtaa cttccctctt ttattatata gaacttttca tcattatttg
    28561 taagaattgt gttaaatttt tctactaaat cttcagacac ttcataacaa catggtccaa
    28621 ttgatggtcc aattatacat actaaatctt caggaactgt tttatagcat tcagacatta
    28681 attttattgt tttatgacct atattactaa aagtccctct ccaacctgca tgagccagtc
    28741 ctatggcttt atttttagca tctataattg atataggtac acagtctgct gtaaacagta
    28801 aaagtggtac tttttccaag tttgttataa gtgcatctcc atctcttctt ttccctatag
    28861 tatctttatt gactatatta actatatctg aatgtatttg agaattacta gttagatttt
    28921 caaatgtcaa atcacattct ttacatactt ttaacatatc ttctctattt tttgcatcta
    28981 tattcttaca tgttataact agatttacag aatttagttt ttcatcaagt tcttcacaat
    29041 taccacttaa attaacatac tctttaatat cttgtacatt ttttatattt atataatctt
    29101 tcatcatttg tctccttttt ctatcagtat acttttaagt tcatttacaa aagtatttat
    29161 atctttaaat tgtctataca cagaagcaaa tcttacatag gcaacctcat ctaactcttt
    29221 caatttatcc attaccatct caccgatttt tttggtttca atttcttcta taaaacattt
    29281 atttatttca ttttcaatat gtacaacaat actttcaatc tcgtcaatag aaacaggtct
    29341 tttttcacat gcttttaaca atccatattt tatcttttct ctatcaaaat attctctact
    29401 attatccttt ttaatgacca ttaaaggagt tgtttctatt ttctcatacg tagtaaatct
    29461 ctttttacat gattcacact cacgtcttct tctgatagat tttaaatctg tatgtctaga
    29521 atctattacc tttgattctt tataattaca ataaggacat tgcatctata ttcctccttt
    29581 gtttaactat atctcaaact catctaagct taattctgaa cctatattta ctattattgt
    29641 atcacaacct attttaagta tatcattcca aaatataact tcttcatccc ctcctcttga
    29701 aaaaaaactt ctacttccat ctccaaaaat ggtaaatgag actaccttac cttcgtgaat
    29761 atccatgtct atgtctatta taaaccccat cctttttcca ttttttacat ttataatttc
    29821 tttttccatt atatcagaaa ctctaatcat attgtactca ccctttcttt aaaaataaat
    29881 atgtatcgaa gtaaaaaaaa atgacctata taggtcattt aaattaaaat aatgtttaca
    29941 gtccatggat tacttataca tattttctca tattctttaa agcatttttt tcaattcttg
    30001 atacttgtgc ttgagatata ccgatttcat cagcaacttc tatctgagtt cttcctttgt
    30061 aaaaccttaa atctaatacc aacttttctc tactatttaa tttcttaata gcttctttaa
    30121 gtgatatttc ttgcaaccaa ttttcatcag tatctttttt atcttgaacc tgatccataa
    30181 cgaatattgc atctccattg tcttgatata ctgggtcaaa cagagatatt gggtcttgta
    30241 tggcatcaag tgccattaca actgattcta cttctaattc taattccttt gctatttctg
    30301 atactgtagg ttctttagaa tttgttctaa ttaatctttc tctaacttgt agtgccttat
    30361 atgctatatc ttttaatgac ctacttactc taattgggtt attgtctctt aggtatcttc
    30421 taatttctcc tataatcata ggaacagcat aagttgaaaa ccttacattt tggcttaaat
    30481 caaagttatc tatagctttt ataagaccaa tacaacctat ttgaaataaa tcgtctatat
    30541 tttctcctct gttgttaaac ttttttatta cactcaagac taatcttaaa ttccctctta
    30601 caaactgttg tctggcttct tcatcaccat tttttatttg taataaaagt tccttcattt
    30661 gcttattttt aagaactgga agttctgatg tattaacacc acatatttca accttattaa
    30721 cttgcatata ttttcagtcc tctctcaaaa gtattcatct attgaaatta tttacatttc
    30781 caattttttt atacttttta agaagaatta atttagcatt atacaaattt tttcatttct
    30841 ttttgcaacc ttgagattat tttcttttca agtctagata tgtaggactg tgatattcca
    30901 agcattccag caacttcttt ttgtgttttt tcaatacctt tatcaataag accaaatctt
    30961 aattccataa tttgcttttc tctgtcactt aatctgtcaa gtgccataac caacaaatct
    31021 ctatctattt cttcttcaat tattttgtat atttcatcat tttctgtacc caatacatca
    31081 gataataaaa gttcgtttcc atctaaatct atatttaaag gttcatcaaa tgatacttct
    31141 gttttctttt tattattttt tcttaagtac attaatattt cattttctat acatctcgaa
    31201 gcataagttg ccaattttat atttttattc agtttaaaag tattaactgc ttttatcaaa
    31261 cctatagttc ctattgaaat caaatcttct acatctatac ctgtattttc aaattttctc
    31321 gatatgtaga ctactaatct aagatttctt tctattaaaa ttgactttac actttcatct
    31381 gtttctagct tttgtaaaag ctccatttct tcttctggtt tcaatggagg aggaagtata
    31441 tttgcccctc ccatatagta tattcccttt ggctttatta tttttaattt aatacctaac
    31501 atagtaataa agcttattat tctttctttc aatcgtaaca ttccttgtcc cccttaaatt
    31561 ttcacctaaa atccaggcaa tatatttgga ttcattattg ctccatattc tctatcatta
    31621 aaatttgaaa taccaagtat tatatttcct atcttttgct catctacttc tatatagtca
    31681 gcttttaagc ctattattat gcttgtctta ttacttccag catgtttata aggtattatt
    31741 ctaactctac ctgaagtttt atcatctaaa cttcctataa tttcttgtgc tttcttcata
    31801 tctatatctt cataattaat atttaatagt ttttctggaa taaatccttc taacacacta
    31861 gacttaacta ttattacatc actttgactt attgggtctt ttaacaaatt accactatct
    31921 actaaagctt tacagcaaaa acttttttct agtaagttta tatttatagt ctttgtaagc
    31981 tccttaatgt acttaagcat tttcaaatct ttataaatgt attttaataa ttcacaactc
    32041 acataagtac atgcaattaa gaaagatatt tttaagtgac ttattcctgt aaaataaatt
    32101 ataaaataag tgcttccaga aataaaaatg tttactagat aaaaaactaa cgctacatga
    32161 acataatctt ttttgctctt acttctaaat gaaattaaag ttattatact catgattatt
    32221 attttaaatg gtacagtaaa tagtatattc attgatggat acaaataagc aacagaatag
    32281 gcacctccta aactagcccc caataactta ttttttttag gattatatct tttagtaaga
    32341 atagaagtac aacttataat aatataattt attactaggt tttctataac atagtactca
    32401 atatacacca tgtatccccc cttaaaacct ttaaacatat tataagatag tatgtacaaa
    32461 attttatgtc acttttgatg gtcgaatcca aaacaaattc tatttaaaaa gaacaatgat
    32521 atatgtctta tactttatgt cgtcaaaaga tattttttta atgccaaaca aatagacttt
    32581 tttgtagaat ccaatatatc taggtagaag ttattcgctc tacttttggt atatttacaa
    32641 aaaaagacta cataatttaa taatatatca agtaaatctt ttacttatat aaaatcaaat
    32701 tatatagtct gtaaattcaa atataaatta ttgtgctagt aatccatttg catattttgc
    32761 agattcattt agtgcttgtt ctggagttat gtctccagct atcatcattt gaagttgttt
    32821 ccataaatat tcatatatct ctactccatg aggtccaact gtcaatggtc tatacatatg
    32881 tttatctctt gtcaccattt cttcaaatct tgggtctcct tggtattttt cacctttagc
    32941 aactggagct ctaggtattt ctttgtggaa agcttccatt gattctttgc ttagcatata
    33001 ctcaagaagt ttaaatccaa gttctttatt tttacatgct gacattaatg ttaaatcatc
    33061 tactgaagca aaacttccaa ctgtcttatc ttctaatgat gttacatatc cccagtttaa
    33121 atccgggaat gatttatcaa aagtacttgt agcagcagag gatagcatta tagtaaatgc
    33181 agcttttcct ggtccaaata cactttcaat catttcatta ctatcttttg ataaagcatc
    33241 ttctggaata tactttttca tatcatttat aaagtttgca gctttcaagc cttctgcatt
    33301 attaaattta actgatttta aatcatcatt atatatatct ccaccagctt gccataaata
    33361 tggataccag ttccagttta agcttccaaa tactttagaa ccccaacctt gtgcaagacc
    33421 ccactggtcg atttttccat ctccatcagt atcttttgtt gctttttcac agattctttt
    33481 aaaatcatcc catgtttttg gaggtttttc tcctaatttt tctagtatat ctttattgta
    33541 gtacaatacg cctggattag cagcttctat aggtaaacca taaataccac ccatcatttt
    33601 tgcatcatct aagtatagat aattatctga agttcctgat ttttctaaat aaggtgttaa
    33661 atcttctacc gcacccattt ctataaattg aggaaacatc tctgcataca tgtatcctat
    33721 atctggacct tcacctgcac ttattgctgt tgcatatttt tctgcgtagt tatcccatgg
    33781 tactatctga aattcaattt cacagttatt ttctttttca aatttttcaa atatcggctt
    33841 ccatgcatct gcatcatggt ctgttaatgg tgcagaccat acaactaatt tttctttacc
    33901 ctctttgcct ttagaatctt ctttttttga acatcctgca agaattgata ctgatagaat
    33961 ccctaccata actaaagaaa gaaccttttt tagtttaatc atgcttaatc ccctttcaga
    34021 ctttaatttg tctttttcct caaatgttta catttaattc agatttaata ttaacaaata
    34081 agattactct tgtctaatca taaaaaacta tactaactcc tataactata ttttttttct
    34141 atacctatta gatttaattc atactaaaaa cttacttttt aaattttatc aatatttaaa
    34201 aattttttta agtctagcca caaactaact atctaaattg agcaaaataa aaagaagtct
    34261 attgacttct ttataagctt acatattatc ttcttcttct taggaatgta ggtatctcca
    34321 tgctctcatc atcatcttct tcaattatac ttgccttttt aggaggctct attttttcct
    34381 cagcaattac ttcttcttca atttctttta cagcttgttt tactgttgta tttaatgaag
    34441 aacgtatgct tgatttaggt ttagtatcta aatctaaggc accattttgt aatccttcaa
    34501 atcctgtcgc gattactgta atcattattt catcacctaa atcttctttt atagaagctc
    34561 caaatataac atttgcttct gggtcacagg attccataac taaagttgat gcctcattaa
    34621 cttcaaataa tcctaagtta ggaccacctg taacattaag aagtactcct ttagctcctt
    34681 gtattgatgt ttctagaagt ggagattgaa tagcttgtct tgcagcatca attgctctag
    34741 tttcaccact agcactacct atacccatat gagcaagtcc cttgtctttc attatagtag
    34801 taacatctgc aaagtccaag tttattaacc cttcaactgc tataaggtct gaaattgatt
    34861 gtataccttg ttttaataca tcatctgcaa ctgcaaaagc atctaacatt gatgtatttt
    34921 tttgtactat ctgtaaaagt ctatcatttg gtatcgttat aagagtatct acttttgatt
    34981 taagttctgc tattccacct tcagcgttct tcattcttat cttaccttca aatgcaaaag
    35041 gtttggtaac tacacctact gtaagtatcc ccatttcttt tgccaagcct gcaaccactg
    35101 gagcagctcc tgtaccagta ccaccgccca ttccggctgt tacaaagacc atatcagctc
    35161 cttgaagtaa ctttactatt tcatctttac tctcctcagc agctcttttc cctacttctg
    35221 gatttgctcc tgctccaagt cctctagtta atttttctcc tatttgaact ttatattcag
    35281 cttttgatgt atataaagct tgtttatctg tatttactga tataaattca acgcctttta
    35341 gttgagcttc taccattcta ttgacagcat tgtttccacc gccacctaca cctattactt
    35401 taatttgtgc acattcttct aattctacgt caaagtttag catcataaac ccccttctct
    35461 catgattcaa agttatcgta tgtattaatt atatcatttt cttcttttat tttgttaacc
    35521 tttaagtaac atattccaca aaaacgtaaa ttttcctttt taaatttctt acttatattg
    35581 tcaaatatta ctctttaagt ataaaataat ttacgtatat attaaatgct ctttaatttt
    35641 tacgtttttt gttcataata ttactaataa tatatcgtct aattatagat aagttattaa
    35701 acaatctcat accaaaaact aatacagcaa catagtaaag agaaactcct aacttttctc
    35761 ctatataagt caataatata gccaatatag cattacctat gaaaccagta ataaaaatta
    35821 cattatcata gttatcttca aaattggatt ttatcgctcc aaaaatagaa tccattattg
    35881 caagaatagc tactgacata tatatggaat aatccataga atatgtaaat ggaatatagt
    35941 aacctactac tacaccaaat gctaatcctc ctaaaaccca taacataaat cttcacctct
    36001 ttattttact ttgaaacaca actacattac aatccttttt attaattttt tttacttttt
    36061 ctttattttt ttgtattttt cgttttattt tgcaaataaa ttcactttat ttccattatt
    36121 ttcctgaact tttatattaa ttccatatat atccttaaga gatttagcat atgattctgg
    36181 agagtttata gctgcactca ataaatctat atctcctata gcttttatta taaatggttg
    36241 tccatatgta gtatcattta ctgtaatagt agctcctgaa cattttatct tacttgttgc
    36301 tagtactctt tctccattta tagaaattgc tcttgctcca gcctttttta aatcatttaa
    36361 aaccctcaaa atatctatat catgtataat caaatcgttt ggattctgtc cgtcttttaa
    36421 ctctctctca ctatccctca tagtaatagt tatgcctggc cctgtaacag ttgaataacc
    36481 acttaactct ttaacttctt caagctcttt ttgcataagt gtttggatag attcatttcc
    36541 attctttttt gcttctttat attcattcaa agtttttttg ttctttttaa gagtttcttt
    36601 taaatttttt atttcttttt ttgtattttc tacctcactt tcaatacttt cagtttgacc
    36661 taatgttatg tatactctat ttgggtctag tgattttata taagttgata tgaaaacgcc
    36721 taatatgaat gctcctaata taactatttt ctttttcata ctattatttc ccctctgctt
    36781 tgtttatatc actatgacca tttatttcta tattgtcttc tttggtcact tttactttgg
    36841 cattataata tgtttctatt tcccagacaa taccatactt tatattaagt gctgaagcca
    36901 atgtatcaga atcaccaatt gcttttatta taattggttc ttttatgaca gtgtcattca
    36961 tataaagctt gtcttctttt aaatacatat atgtattaga aactattctc tggtcgttta
    37021 tagatatggc acttgcttgt gctgaattta atttatttat cattgacaat aataaatcat
    37081 aattatatag tatactatca tttttatcag agtttcctaa gtcttttgta gaatttatct
    37141 caatttttat gcctgttcct tcaacatctg tatatccagc taatgtttca tatttttcta
    37201 tttctgactt aagtatgtct tgttctcctt cattgttttt gcctctataa gtctctatag
    37261 ctttcttcac actattaact tctgattgta gattctcttt ttcttttttt aatgatttaa
    37321 gttcttctgt aagttgctct cctcttttaa aagttgtcat ccctctattt tctatattaa
    37381 taattttaag ttgtaaacta acaagaacac ccaatacaat acttcctatg attatacttt
    37441 tatttataaa tctttttttc aatatatcac ttccttacaa ttgtctatcc ttctggacta
    37501 tacactgcca actttccata gttaagattt atagtccctc cattagtatt tttattttgc
    37561 aggtcaatca gaattttact taccctagat atattataat ctaagttatc atcattagaa
    37621 agtaagattt ttatattact tcttgtaagc atttctatat tgctttcttt ttttaagttt
    37681 acataattta tttttctata aattccttct tcttttaaat aatttaaagt tttaaataca
    37741 ttctccttag ttttataatt tttaaatttt atagatttat tatcatcaat gctataatct
    37801 acatctacta tcaagtcttt tttactttca ttagaacctc ttagttcttc caataaattt
    37861 ccttttttat ctatataaca ataattatct tcatctttta atactgcaaa tatttctttt
    37921 tcttttaaac ttataattat cttattggga agttttcttt ttatttccac attttctata
    37981 tatggatttt ttataagttt atttttcata tctttaaaat tgtatgcaaa aatattttca
    38041 tttagattta catttagttc tttcattata tttgatttgg tcaccctctt atttccaatt
    38101 acatctattt tctttacatc aaataaatca ctttttacaa ttatacatat acaaaaagat
    38161 aacataagta taaaaactaa aactatcatg acattattcg tatttaactt tcttcttttt
    38221 ttcaaatata atcaacccct ttggttaata aatacacctt tagagcttgt ttttcctaca
    38281 aaaaaattat aatatttaaa gtcaaatttt tgcattttat tacataacat ccaaataagg
    38341 tattaaattt tataaataaa tcaatatttc atcaaaataa acatagcata ctacatacta
    38401 tttatatatt taaatctgta tacatacaaa taaaccccta atttataata atttagaaaa
    38461 tctaatatat tataaattag gggtaaatat ttacttatct tttctttatt cctattacct
    38521 tagcttgacc ttctatactt ggagtcgttt ctttttttat ttcttttact tccttaataa
    38581 ctttttcttt ttttgttttt ttagaagttg acttttgtgt tgaattgtat actttcatta
    38641 tctcatcata tataaggtct atggcttcag gcttacctat agttttactt gcatttgcca
    38701 tatcaaccaa aagttctcta tctcctaaaa gtttaaatac tgctgtattt aggctctctg
    38761 gagttagatt tttttctaaa atagcaattc ctgccccttg tttttctata ctcttggcat
    38821 tatactcttg atgattttca gcagtataag ctttaggtat tattatagat ggctttccaa
    38881 gagccgttat ctcagctaat gaaattgctc ctgcgctacc tataaccaaa tcacttgctg
    38941 caagtgcatt tgccatatct tctaagtaag gcacaacttt ttggtatggt ttaagattta
    39001 tatcgctaat acttcccata aattcatcat aatagctttt gcctgtagca aatataaaag
    39061 ctatatcttc atttaccata ttctttatta caagtctcat tgcatcattt atttttcttg
    39121 aacctccact accaccatag caaagtacca ttcttttttc gtcacttatt gatagattct
    39181 ttcttgcaat attttttctt gataataaaa tttcttttct aactggattt cctgtaaaaa
    39241 ctaatttatc ttctgctgcc tctggaaatc ttttatgcga atcttcaaaa cttgtaagta
    39301 cctttgtaac tgttttagat aaaatcttat tagttacacc aggaaaagaa ttttgctcat
    39361 gtattatggc tggtattttc cccatagatg cattaaataa aacaggtcca ctaacatatc
    39421 caccagttcc tataactata tctggtttaa atttcttaac aatctttctt gattgctcca
    39481 aacctttaaa aagcttaaaa actcttttta cattatcgaa atctattttt cgcttaaaac
    39541 cttgaactgt tacagtcttt aattcaaatc catacttagg aactatttct gactctattc
    39601 ccttttccgt cccaacaaaa attatctcag cgtctggatg ctcatctctt attttgtttg
    39661 caatagctat agctggatac acgtgccctc cagttccacc gcctgataat aaaactttca
    39721 tcaaatcatc atctcctgtt aattaatttt gacatgcttt gagatgttta acactattcc
    39781 tacagctccc ataaagatgg tgagtgaagt tcctccataa cttataaatg gtagtgctac
    39841 acctgtagct ggcattgatg atgtcgcaac tgctatattt aaagctgctt gtattccaat
    39901 ttgtgcacct atacctataa caaccataca agcaaataca ttagaagtct tcaaagctat
    39961 tcttacacat ctgtagacta aaactacaaa tagcataata actattatac aaccaatcaa
    40021 tcctaactct tctcctatta tagcaaatat aaagtcattt tgtggctctg gtatataaaa
    40081 atacttttgt ttactctttc ctaagcctag accaaacaat ccacctgaac ctagtgcata
    40141 tagaccttgt attacctgat accccttacc aagagggtct tgaaaagggt ctaaaaatga
    40201 tgtaacacga ctaagtctat agggttcagc aattactaat gcagcaaata aagcagcccc
    40261 actccctatc atagcaaata caattttcat atccattcca gcaacaaata tcattacaaa
    40321 agtaactaga attacagtac ctgctgttga caaatttggc tgaagtatta tcaacgcaaa
    40381 aaatatccct ggaactacga gaagtggtac aacacctttt gtcaaagatt ttattttatc
    40441 ataatttttt tcaattaatt ttgcagttaa tattattgta gcaaactttg cgatttctgc
    40501 aggttgaaat gttaaagctc ctattcccaa ccatcttttt gctccgtttg cttctatccc
    40561 taaaggggtc aaaactaaca ataataatac tactgctata gctccaattg ctgtagcatt
    40621 tttcttccaa aaactataat ctattcttga tgtaataatc ataactataa atccaagtac
    40681 agcatatatt acattctttt ttagaaaata gtacgcatca ttgtgtttaa atgaagattg
    40741 tataaaactt gcactaaaca ccataactat tcctacaaat actaaaagca tagtagtgta
    40801 aaacactact ccatcaaatt cagtcttcat ccttatgtca atttgttttt tcaaattttc
    40861 tttaggcatc caactaatct acctccattc ttccatttaa gtaagtttag aggtaagaga
    40921 ttattttaaa ttgtttacat tatctttgaa gtcttttcct cttacttcaa aactttcata
    40981 catatcccaa cttgcacaag ctggtgaaag aagcactata tctccacttt ttgaaatttc
    41041 ataagaagtc ttaactgctt cttccatatc ttttactata tgtatgtcat taaatccttt
    41101 atttttagca caattttcta tatttgatgc agtttctcct aataagacca atgatttaac
    41161 atatgacttt gcagtttcaa gaagttcatc aaaattactt cccttatcca tcccccctgc
    41221 tataagtatt ataggtctat cataagactg tatagctttt atagtggaat ctggattagt
    41281 tccctttgaa tcatttacat atattacatt gtctaaattt cttacaaact cttgtctgtg
    41341 ttccactcct ttaaatgtca taagtacgtc ctttataact tctaaatcaa ttttgcagac
    41401 atatgctatt gctatagctg ccatacaatt ttctaagtta tgacctccag gtaaacttac
    41461 atcatcttta tttagaaatt tgattttatc atctatatct attattatgt cattattttc
    41521 taaatatact cctccattta cttttttagt tcttgaaaag taaattactt tggcattgca
    41581 tttatcagct aaagatttta ctatctcatc atcatagttt aatacacaga aatcctcaac
    41641 agtttggttc ataaatatat ttgcctttgc ttctatgtat ttttccatag tatggtgtct
    41701 atttaaatgg tcctctgtaa tatttaatat tgcgcttact ttaggtctaa attcatctat
    41761 actctcaagc tgaaaactgc ttagttcagt tactaacaca gactcttcac ttgaagtttc
    41821 tatagtatct attacaggat ttcctatatt accaactaca tatgtatctc gttttgctct
    41881 tgaaaaaatt tctcctacca aactagttgt agttgtcttt ccatttgtac ctgtaatccc
    41941 tataaaaatt ggattattcg atagtttaaa agctaattct acctcaccaa ttatatactt
    42001 gcctgaattt tttaatttca atatgaatgg taggtctagt ggtacacctg gagacactac
    42061 aaccatatct atatcatcta catcttctgg gtgatgtcct aatatgtatt ttatgtcatt
    42121 tatagatttc aattcatcaa gtatatttct caatttattt tcatctttta tatcatttac
    42181 gattatactt gcacccaatt tatctaaatg tttaattgta gatataccag tttttgcaag
    42241 tcctactaat aaaacttttt ttccttttaa gtccattttt cattccccct agccctaaaa
    42301 tactgctatt atacttatcc aagctaaaac aactgttata atccaaaaaa taaatacaac
    42361 tcttgtttct ggccaaccac actgttcaaa gtggtggtgt attggagcca tcttaaagaa
    42421 tctctttctt gttgccttaa aatatccaac ttgaatcaaa actgatagag cctccgcaaa
    42481 ataaattcct ccaattatag gtattataag cactgagtta gttagaactg aaaatgctac
    42541 aactgcacct cctaatgcca ttgaacctgt gtcgcccata aacactctag ccggatatga
    42601 attaaatcct aagaaaccta aacatgctcc aacagttgct gctgctaaaa ctgcgacttc
    42661 tgtatttcca gcaatagaac ttgcaaatag cataaagaat acagaaacta ttaatgttac
    42721 tcctgatgct aatccatcta atccatcagt caagtttaca gcatttacta ttgctacgat
    42781 tataaacatc attattggta tatataatat tccaacattt atcacaaaat ctgtaaatgg
    42841 tatcataagt tgtgaagcac ttgatgatga tgtatactga taaaaagcaa cataaaaaga
    42901 taatgcaact tgaaggatta ttttttgata tgcttttagt ccaagtgatc tttttagctt
    42961 tatttttata aaatcatcta aaaagccaat aaaaccaaaa cctgcaatac atataagacc
    43021 tactgccata tcatgactaa cttttactct tgtaagtcca gttattagaa ttgcaacaat
    43081 cattataatt ccacccatag taggtgttcc gtttttagct agatgtgttt ggggtccatc
    43141 atctctaact gtctgaccaa atttaaattt tctaagcatt ggtataaata ttggtcctat
    43201 tataattact attaaaaacg caatcaatgc ggtgtacgta agctctgtta ttcctaacat
    43261 cataaaactc ctctcccttg tcaattaact cattagttta agcttattta tttagatatt
    43321 cgactatttt ttctaatctc atgcctcttg agccttttac taaaatagtg tctcctgttt
    43381 ttactaattc atttaacttg ttaaacacat cttctctatt ctcaaagtga tatatattgg
    43441 ctgaattgaa tcctaattgt tttgcttctt ctccaataaa cactgaattt tcacctatag
    43501 ttattataat atcagtatta ttcatagatg attttccaac taatctatga ccatactctg
    43561 aaatttcacc catttctagt atatctccaa gtatcgctac ccttctacct ttatatcttc
    43621 caagtatctt taaagcagca tccatagaat ctggactagc attatatgaa tcattaatta
    43681 ttgttaattt ttcatttttt attatgtcta gtctcatttt tgtcccttta aaatttttta
    43741 aacctttttt tatataatct aaagaaatat ttagacacaa accaactaat atagcagaca
    43801 ttgcattata tatattatgt tctcctattg ttggtataaa tatttcctct tttttcccat
    43861 ttattacaca tataaatgtt aaactatcct cttccatagt ataactttca caataaacat
    43921 cattgttttt attaaagcca aatgttttta acttatatac atgttctttt tcttttaatg
    43981 tagataaaaa cttatcatct ccatttacaa ttaaagtatt tgtttcatca aagtttgaag
    44041 taatctccat ctttgctttc aaaataccct cttgtgaacc tagtttttct acatgtgata
    44101 atcctatatt cgatatgact gcaattttag gatttactat atcaactaaa tattttattt
    44161 cattgaatcc agacataccc atctctatta cagcacactc atgttcttta tttagattaa
    44221 ataaagtcaa tggaactcca aaatgattat ttaagttttt ttcatttttt agtatattta
    44281 actttgcaga tatggcagag taaatcatat ctctagtagt agtttttccc acactacctg
    44341 taacacctat aaatggaata tcaaattttt ctttatagta ttttgaaata tctcctagag
    44401 ccaaagatgt atcttttacc tctattaaat ttatatccga actttctaat tttattccat
    44461 tactttcatt ttttataaaa gttttacatc cactatcata tgcagacttc ataaatgtat
    44521 gcccatctaa attttctcca actatggcta caaatgcatt ttgggcattt gcctccctgc
    44581 tatctataac tatatcactt acacagtcat tataatctcc atatattaac ttcccattag
    44641 ttgcaagtac taactctttt attgttaagc actccattat taaactcctc ctttaaaacc
    44701 ttagctatat tctcttagca acaaaaggca atgtataccg ttatatatac attgcctacg
    44761 ataactctta actttataat tatatactat atttctgttt ttttgaagat gtttatgggt
    44821 atatgttaat tatctgttac tttttagttt tcaaaatata gggttatact tgagtcttca
    44881 ttaactttta ctccaggttt tgggaatata tcctttacct ttgtatcttc tggaagttct
    44941 atatctgcat ctaagtctgg ttttaacttc gcatcctcca atgcctttac agcatctcct
    45001 atttttagat ttcttacatc tggcacttta acttgcttct tttcatactc agctttttct
    45061 tcttctttat atactggttt aactcccaaa tattttaaag aatcattcat tatttctttc
    45121 actattggtc ctgctgtagt actaccaaat gcacttactc ctgttggttc gtcaacaata
    45181 gcaagaacta ctatttgcgg gtcatcacaa ggtgctattc caacaaatga acatatatac
    45241 tttcctggag catacttacc atctataact ttttgagctg tacctgtttt accaccaagt
    45301 ctataacctg gtatatatgc tatctttcct ccaccttcag ttacaactga ttctgcaatt
    45361 tctaacattt ttttagatgt ttctttagat ataacacttc ttactttttt tggttttact
    45421 gtttcagtta tatttccttt attgtcagta taagatttta ccactcttgg ttgcattaaa
    45481 tctccaccat tagctattga tgatatagct gttataagtt gtattggagt tactgatata
    45541 gattgaccaa atgatattgt agccaattca actggtccta catttttttc gttatacagt
    45601 attcccttag cttctcctgg taaatctata cctgtcttat ccataagacc aaatgattct
    45661 atataatcat acatttttcc aacacctaat ctgcttccta actcaacaaa tactgggttg
    45721 caagagtttt gtactgcttg tttaaattct tgtgttccat gaggtctgta atgtctccaa
    45781 catttaatct ttcttcctcc aacagttaca ctacctgtac atgtgaattt ttctccatct
    45841 ttaattactc cttcttcaag agcactggaa gatgtaataa gcttaaatgt agaacctggt
    45901 tcatatgtat cacttactgc tggatttctc cacatttgat aatagccttt tattttatct
    45961 ttatcattat atttttcaag ttcttcttga taatatggat atattggcgt tcttgagtca
    46021 ttagggtcat aatctggttt tgatgctaaa gcaagtatat cacctgtttt agggttcata
    46081 gctattgcag taactttttt tgcattattt aactcataag ctttctgtac tgctttttca
    46141 gtatagtgtt gaattacttc atctatagac aacacaagac cattaccttg tacaggctca
    46201 taatattttt ccattccttg tggtatttct cttcctgaag catcagtact tactataagt
    46261 tttccaggct ttccttttaa ctttttatca tattgcattt ctacaccaga tatacctgtt
    46321 gcatctgaag aagtatgtcc taacacatat ggtgcaaaat tcccatatgg gtaatatctt
    46381 tgattatctt ctgccaccca tattccactt aatttagcat ctcttatttg acttgcctta
    46441 tcatcatcta tccatctttt tacttttaca agagccatat tttttttgct tattaaagcg
    46501 taaatatctt tataatcttc atccaaaatc tcagctacct tttcggctgc ttctttttta
    46561 tcttctactt ccacaggttt acaccaaaca gtatacttag ttacacttac tgctaactct
    46621 ttcatatttc tatcatatat agttcctctt ttgggttcta ttggtatatc tcttgtttgt
    46681 tgttctagtg cttttgtact caaccaattt cccttcataa gttgtagata cccagtccta
    46741 ataacaaggc aaaaaaacaa tgcacatgct agaataagaa cgagtaccag ccttttctta
    46801 cttatcctct ttacttttct caaatctatt ccccctagtt atccacatct atatacttta
    46861 tttgtccatc ttttggatag tccatattga gcaattcctt agcctttagc tcaatttctt
    46921 ttattgatgt tttttcagtt tcttttgcct ttagttcctc taatactatc tctttttttc
    46981 ttaaatcttt ttgtaaataa tagatatcgt acttcatttc cgagattttt gaataaccac
    47041 atagattcaa tattattact gaaaaagttg caatcagtaa aatgcattta gctcctattt
    47101 ttttcttatt tctcttttta taattattct tttttttatt tttatattca tttagattat
    47161 gtattttata attttttttc aaattctcac cccgatagta tatagtttaa attttcctct
    47221 atactatatt tgttattaat catatttctt cctctaaaat ttcatcgcta aattaataca
    47281 ctattacaca atctaattgt atttttttct atacacaaca aaaacctata aattcactta
    47341 tacttttata tgaatctata ggttagatat taatttctat ttatttttat cacttctagt
    47401 atttttagat aatttcttcc ataaatataa tatgtaacta actatctact ctctgattcc
    47461 taagttctcc attatattac ttaataccat ctatatctat atttttatat ctaaattttc
    47521 attatatatc tcaataacaa agaacatttc ttatagtgtt aattgttttt taattgcttt
    47581 accagtgcaa caaaatatat aatacttttt aattaattat aataatctta tatataagat
    47641 tattctttag ccttataact acttatattt tttctgctac tcttagctta gcacttcttg
    47701 aacgtggatt tacttctatt tcttcctcac taggtaatat aggttttcta gttattattt
    47761 tcacctcaga ttctttatca cattggcata taggtaaatg ttgtggacat atacaatctg
    47821 atgctaaatg tttgaatgca tttttcacta ttctatcttc taatgaatgg aatgttatta
    47881 tacatattct tccaccttca ttcattatag aagaagcatc atttatcatc tttgtaatta
    47941 ctccaagttc attatttact tctattcgta ttgcctgaaa agttctttta gctgggtgag
    48001 gtccatctat tctagctttc ttagggatag cttttttaat tacatctact aattctcctg
    48061 tagtttctat tggtttgttt gctctttctt caactataaa tttagctata cgcttagccc
    48121 agttatcttc tccataatct tttattatct tagttaattc atcttctgta taagtattta
    48181 caacatcata tgcagaaaac tcacacctaa catccattct catatcaagt ggtgcatctt
    48241 gcatataaga aaatcctcta tcagcttcat caagttgatg agatgaaact cctaaatcag
    48301 ctaatacacc atcaattttg taaactccta ttttttctaa ttcctctttt atattttcaa
    48361 aattgctgtg aacaaacttc actctacttt catattcact caatctctct tttgctgtgc
    48421 ttatagcatt tttatcttgg tcaaacccta taaataatcc cttatctgac agttttttta
    48481 ctatttcttt agaatgtcct gctcctccca tagtacagtc aacatacacg ccatctggct
    48541 ttatatttaa attctctata cactcattta aaagaactga tacatgatga aattccatag
    48601 ttacctccct taaattcact ttttaaacta ttcttcacta atattatttt tatttttctt
    48661 tgataaataa acatgtgcaa ttatgccacc aacaactcca attagactta taacttgtgc
    48721 cattctaagt ggtcctaaca ttaaactatc tgttcttaat ccctctataa aaaatcttcc
    48781 tattgaatat aaagttatat aagtaactat aacttgacct tcatattttt tattttttct
    48841 aaatagtaat aaaactataa atattccaaa atcccatata gactcatata aaaaagttgg
    48901 atgtaccttt actccatcaa ccattattcc ccaaggtaaa ttggtagcac ctccatgtgc
    48961 ttctccatta ataaaatttc cccatcttcc aatagcttgt gctaaaggca ttccaagtat
    49021 aactgtatca gccattttta agaagttaat atttttaatc ttagtgtata tataaccagc
    49081 taatattcca ccaattaacc ctccatgtat agcaagtcca ccacctctaa aattaaaaat
    49141 ttgagacata ttttgagcat agtaactcca attaaataca acataataga ttcttgcaca
    49201 gattagtcca actggaattg ctattatagc tatatttaat acatcatctt ctttaattcc
    49261 cactcttttt gcttctttta ttgctattaa agttcctaaa atcattccac atgccataag
    49321 tattccatac cacatgatgt ctatgccaaa cagtgtaaat gctactctat ccataacgtc
    49381 acctctttgt attaattgaa aatatttcca aagcaatatg ctataattac aattaattgt
    49441 aattatacta aattatgata acacattaat gttaattcta caaaaaatat taaaatttat
    49501 tctacaactc cctacactat attatatcta attactataa aattttttaa actgaatctc
    49561 tcatgactaa agttacaagt gtacatagat attttttaat caaaaagatt gatgcatata
    49621 tacatcaacc tcttctaatt cttatctata gctcttaaat ttttacctca atttatataa
    49681 atatatttta taaatttgtt ttatcaaaat aaacatttaa aaacataaac taatacttaa
    49741 ttagttattc ttattatgat taagtttcta tttaggctct acaatagaaa taacacaata
    49801 ctcttcttta aaatgctctt ttaatctttc ttcaacttcc tcaaaagtaa tatctttaat
    49861 aacatctaaa tactccaata gatttatatc cttaaaaaca taagatataa aactattacc
    49921 tataaagttt atagaatcaa agcacttaat aaatgaccct atttttttct ttttagttct
    49981 ctcaaactct tcttttgata atccttcttt ttttgatttt tctatataat caagaattat
    50041 ttcttttact tttttaggct ctttagaatc tccagctatt atgctaaaag cataatccac
    50101 ttgagacgaa aatccagctc caaaattttc atttattaaa ccttgcatat acaaatcttc
    50161 ataaagctta cttccttttt taaataacat ccctacaagt atatcagtta caatctcttt
    50221 acgtaaaagt tctttccctt ttaaaccaac attactatct ttaaaaccta tgttgaacat
    50281 aggcatagat atagggaatt tttctataac ctctttttct tttacacctt ctggttcttc
    50341 tggataaaat ctttctatac tcttagagag tttatctact ttgtaattgt ttgacttttt
    50401 agttacatct ataacttttt ctacatctaa gtcaccaaca acaaataaag ccatatttcc
    50461 tggattatag aaagtattat aacatttata tagctcttcc ttagttatct tatatatact
    50521 atcaacagta cctgctatat caattctagc aggataattt acatacattg ccttcaagca
    50581 attaaagtat acattccaat cagggtcatc attgtacatt tttatctctt gtgctattat
    50641 acctttttcc ttttcaacat tttcatctgt aaaataaggt gtttgaacat agtcaattag
    50701 atgttctaaa ctctcataaa aattttcagt agcagaaaat aagtaggctg tcattgtaaa
    50761 attagtaaat gcatttgcat ttactcctaa ttttgaaaat ttatcaaatg catctcctcc
    50821 atcaggttgc tcaaacatct tatgctctag aaaatgtgca ataccttcat tcactctaat
    50881 tttcttatct tctccaattg gtacaaattc taagtcattt gaaccataat tagtagccaa
    50941 aatagcatac tttttcataa aacccctttt aggcataaaa tatacatcta atccattttg
    51001 caatttctca taataaactt cttctttcaa tatatcatta actatttttt ccatagacat
    51061 ctcctccatt aatttctcaa gaagtataca gtatcaagct ctatattttt tatagcttca
    51121 actatatctt tttttgtaac cttttctata ctagatatta tgtcttgtac ttcagaattt
    51181 gtctttgcca tagattgaga gaaaacaaaa tcagacatac cacctatatt atcagttata
    51241 gatttcatag aattaactaa agcaagttta ctgttttcta attcttcatc agatatatca
    51301 cctgctttta cactttctac ttgttttttt atcaattcta cagctttatc ataatcttta
    51361 gtttctatac cagatgatat aaacatgata cttttatact tctctattga agagaatata
    51421 tagtaacata aactttcttt ttctcttaca ttaacaaata atttagagtg tggaccacca
    51481 cctaagacat tgctcccaac tactaaagca taatatttat ctatgtctgc ataatccaca
    51541 tttgctctat aacccataac taacttacct tgagttattt ccatttgttc atctataact
    51601 tttacctcat ccacattttt tataaaatct gctcttggta tatttataat ttcttctctt
    51661 tcaaatttta gatttttact tattatatct acaactttat cttcatcaaa attaccttct
    51721 acaactatat ctataggtga tgttttaagt atgtttttat aatgttcata taactctctt
    51781 gaagttatac tatctatttc atcttcataa ccaaattcac ttacactata tctctctcct
    51841 ttacacatag cctcaataca tttatcttgc gcatattcct ttttatcatt tattattgat
    51901 tgtatctttt ctcttaaatt ctctttttca atatttaaat attcttcttt aaacccacca
    51961 tctacaacaa gcgtattatt tattacttca ttaaaaaatt ctactacttc ttcaaatata
    52021 ctctcatcta aatacttttc acttatgtta attactttaa aactcaatac ttgacgttct
    52081 ccacgtttta cagcatcagc tcccatagat gaaccatata aatcatctaa tttattggat
    52141 attgctctag cactaggata tttagcgctt ccacttgcta ttattgatgg tattagagca
    52201 ttttttgtag tttctttttc atccaatagt ctctgaacat ataaacttat aagattcgat
    52261 ttaaactttt ctgtttttat caaagtaaga ttaatattat tacctaaatt tattattttt
    52321 ttagtattaa ccatatgtac ctcctatttt tatttaaaca tcaattttaa aattgaaatt
    52381 tatttatttt ttaattacat atactcacat ataaaaacct tctatttaat tatatccttt
    52441 ttattatttt ctaaaagcaa tatttttatt taaattaaat acaaatatta tttaaatcaa
    52501 tatgctttaa agtctagttt acaagcttga tatatatgat gtataatcaa tatgtatgtc
    52561 tataattaaa acaattttgc aatcctatag attgattgtt taaagttata taaagaatat
    52621 aaaaaagaat aagagagagg acgattaata tgaaattagg tatagttggt ttaccaaatg
    52681 taggtaaaag cacactattt aacgcaataa ctcaagcagg tgcagaatct gcaaattacc
    52741 ctttttgtac aatagaccca aatgtgggag ttgtatctgt tccagatgaa agattaaaca
    52801 aactacaaga attatataat tctgaaaaaa tagttcctac tgctatagaa ttttgtgata
    52861 tagcaggttt agttagagga gcttctaaag gagaaggttt aggaaataaa ttcttatcac
    52921 atataagaga agttgatgca atagttcatg ttgttagatg ttttgaggat gaaaatgttg
    52981 tacatgtaga tggttctgta gacccacttc gtgatataga aactataaac ttagaattaa
    53041 ttttctcaga tatagagatt cttgaaagaa gaattaataa aactcaaaaa gctgctaagg
    53101 ctgataaaac tttaggttct gaacttgatt tattaaaatc aataatgtct actttagaag
    53161 aaagtaaatg tgtaagaact atggaattta cagaagatga acaaacattc gtaaattcat
    53221 tggatttact tacatctaag cctgtaatat atgcttcaaa tgtatcagaa gatgatttag
    53281 ctgataatgg agaaaataat aaatatgttc aacaagttaa agcatttgca gaaacagaag
    53341 atgccgaagt tgttgtagta tgtgcacaaa tagaagcaga aatttctgaa cttgattctg
    53401 ctgaagaaaa gaaagagttt ttagaaacat taggtcttga acaatcaggg cttgataagc
    53461 ttataaaatc ttcttatgct ttacttggtt taatttcatt cttaactgct ggtccaaaag
    53521 aagttagagc atggactata aaagttggaa gtaaagctcc tcaggcagga ggaaaaatac
    53581 attctgatat agaaagagga tttatcagag cagaaacaat agcctttaat gatttggttg
    53641 agcatggtac tatggctgct gctaaagaaa aagggttggt taaattagaa ggtaaagaat
    53701 acatagttaa agatggagat gtaatattat ttagatttaa tgtttaaaat gattaatcgt
    53761 atacactttg actattatat ataataacta aa
//
LOCUS       DAMJYC010000019        53210 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJYC010000019 DAMJYC010000000
VERSION     DAMJYC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.020"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            60..869
                     /locus_tag="PC472_001199"
     CDS             60..869
                     /locus_tag="PC472_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2726534.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1014..1253
                     /locus_tag="PC472_001200"
     CDS             1014..1253
                     /locus_tag="PC472_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726535.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDRKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNIDRNWCKDKK"
     gene            complement(1440..1616)
                     /locus_tag="PC472_001201"
     CDS             complement(1440..1616)
                     /locus_tag="PC472_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726536.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1670..1766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2121..2795)
                     /locus_tag="PC472_001202"
     CDS             complement(2121..2795)
                     /locus_tag="PC472_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2726537.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3015..3569)
                     /locus_tag="PC472_001203"
     CDS             complement(3015..3569)
                     /locus_tag="PC472_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2726538.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3647..4012)
                     /locus_tag="PC472_001204"
     CDS             complement(3647..4012)
                     /locus_tag="PC472_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726539.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4477..5262
                     /locus_tag="PC472_001205"
     CDS             4477..5262
                     /locus_tag="PC472_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726540.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5359..6183)
                     /gene="pdaA"
                     /locus_tag="PC472_001206"
     CDS             complement(5359..6183)
                     /gene="pdaA"
                     /locus_tag="PC472_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2726541.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6404..9523
                     /locus_tag="PC472_001207"
     CDS             6404..9523
                     /locus_tag="PC472_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2726542.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9541..10140
                     /locus_tag="PC472_001208"
     CDS             9541..10140
                     /locus_tag="PC472_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2726543.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10367..12496)
                     /locus_tag="PC472_001209"
     CDS             complement(10367..12496)
                     /locus_tag="PC472_001209"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2726544.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLARPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12762..13010
                     /locus_tag="PC472_001210"
     CDS             12762..13010
                     /locus_tag="PC472_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726545.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13176..14567
                     /locus_tag="PC472_001211"
     CDS             13176..14567
                     /locus_tag="PC472_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2726546.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14682..16136
                     /locus_tag="PC472_001212"
     CDS             14682..16136
                     /locus_tag="PC472_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2726547.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16201..16794
                     /locus_tag="PC472_001213"
     CDS             16201..16794
                     /locus_tag="PC472_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2726548.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16976..18322
                     /locus_tag="PC472_001214"
     CDS             16976..18322
                     /locus_tag="PC472_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2726549.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18504..18875
                     /locus_tag="PC472_001215"
     CDS             18504..18875
                     /locus_tag="PC472_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2726550.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18868..19557
                     /locus_tag="PC472_001216"
     CDS             18868..19557
                     /locus_tag="PC472_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2726551.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20077..20943
                     /locus_tag="PC472_001217"
     CDS             20077..20943
                     /locus_tag="PC472_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2726552.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21185..21994
                     /locus_tag="PC472_001218"
     CDS             21185..21994
                     /locus_tag="PC472_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726553.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22138..23202
                     /locus_tag="PC472_001219"
     CDS             22138..23202
                     /locus_tag="PC472_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2726554.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGAIFGGSYKTYYTQNFYK"
     gene            23230..23949
                     /locus_tag="PC472_001220"
     CDS             23230..23949
                     /locus_tag="PC472_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2726555.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23973..24557
                     /locus_tag="PC472_001221"
     CDS             23973..24557
                     /locus_tag="PC472_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2726556.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24559..25908
                     /gene="pabB"
                     /locus_tag="PC472_001222"
     CDS             24559..25908
                     /gene="pabB"
                     /locus_tag="PC472_001222"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2726557.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25898..26641
                     /locus_tag="PC472_001223"
     CDS             25898..26641
                     /locus_tag="PC472_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HDF2726558.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDISYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEDVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26675..27376
                     /locus_tag="PC472_001224"
     CDS             26675..27376
                     /locus_tag="PC472_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726559.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27402..27974
                     /gene="folE"
                     /locus_tag="PC472_001225"
     CDS             27402..27974
                     /gene="folE"
                     /locus_tag="PC472_001225"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2726560.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28038..28829
                     /gene="folP"
                     /locus_tag="PC472_001226"
     CDS             28038..28829
                     /gene="folP"
                     /locus_tag="PC472_001226"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2726561.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28840..29205
                     /gene="folB"
                     /locus_tag="PC472_001227"
     CDS             28840..29205
                     /gene="folB"
                     /locus_tag="PC472_001227"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2726562.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29198..29704
                     /gene="folK"
                     /locus_tag="PC472_001228"
     CDS             29198..29704
                     /gene="folK"
                     /locus_tag="PC472_001228"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2726563.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29685..30470
                     /gene="aroF"
                     /locus_tag="PC472_001229"
     CDS             29685..30470
                     /gene="aroF"
                     /locus_tag="PC472_001229"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2726564.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKRQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30725..32515
                     /locus_tag="PC472_001230"
     CDS             30725..32515
                     /locus_tag="PC472_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2726565.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32533..33699
                     /gene="rpoD"
                     /locus_tag="PC472_001231"
     CDS             32533..33699
                     /gene="rpoD"
                     /locus_tag="PC472_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2726566.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33864..34553
                     /locus_tag="PC472_001232"
     CDS             33864..34553
                     /locus_tag="PC472_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2726567.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34567..35664
                     /locus_tag="PC472_001233"
     CDS             34567..35664
                     /locus_tag="PC472_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2726568.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKFDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35727..36299
                     /locus_tag="PC472_001234"
     CDS             35727..36299
                     /locus_tag="PC472_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2726569.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36359..36826)
                     /locus_tag="PC472_001235"
     CDS             complement(36359..36826)
                     /locus_tag="PC472_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2726570.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37212..37688)
                     /locus_tag="PC472_001236"
     CDS             complement(37212..37688)
                     /locus_tag="PC472_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2726571.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37808..38899)
                     /locus_tag="PC472_001237"
     CDS             complement(37808..38899)
                     /locus_tag="PC472_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2726572.1"
                     /translation="MSKIECLFTIFPVLILMYTTFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38892..39281)
                     /locus_tag="PC472_001238"
     CDS             complement(38892..39281)
                     /locus_tag="PC472_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2726573.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39473..39919
                     /locus_tag="PC472_001239"
     CDS             39473..39919
                     /locus_tag="PC472_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726574.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40183..40908
                     /locus_tag="PC472_001240"
     CDS             40183..40908
                     /locus_tag="PC472_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726575.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40895..42139
                     /locus_tag="PC472_001241"
     CDS             40895..42139
                     /locus_tag="PC472_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2726576.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLIIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42219..42896
                     /locus_tag="PC472_001242"
     CDS             42219..42896
                     /locus_tag="PC472_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726577.1"
                     /translation="MEILRVENITKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42897..45458
                     /locus_tag="PC472_001243"
     CDS             42897..45458
                     /locus_tag="PC472_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726578.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRRNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45679..46953
                     /locus_tag="PC472_001244"
     CDS             45679..46953
                     /locus_tag="PC472_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2726579.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(47114..50155)
                     /locus_tag="PC472_001245"
     CDS             complement(47114..50155)
                     /locus_tag="PC472_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2726580.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDLANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50362..50661
                     /locus_tag="PC472_001246"
     CDS             50362..50661
                     /locus_tag="PC472_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2726581.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(50899..53127)
                     /locus_tag="PC472_001247"
     CDS             complement(50899..53127)
                     /locus_tag="PC472_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2726582.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        21137 a         5723 c         8746 g        17604 t
ORIGIN      
        1 atagagtaat aatgttgatt taaaataaaa ataggatttc ggaggagttt attatataaa
       61 tgaaagatta ctataaaata ggagagatat ctaaaatata tggaataggt agagattcat
      121 taatgtatta tgaagaaata ggcattctaa gaccagttag agatataaat ggatatagaa
      181 tgtataatat atcagatata tggaaattaa atttaataaa agaatttaga agcttgaact
      241 ttccaatgaa gaaaataaaa gaatacctag atgacagaag tatagagtct acgaaaaata
      301 ttttaaatga agaactcgat ttaattgata aaaaaatagc tgaatttata agccataagg
      361 aaaatattat aaaaagattg agttcaattg aaagtgtaat tcaaaataca aaaatagatg
      421 agatagaggt tgtatatata gaaaaaagaa aagctttaga attaaatgct gatataaaga
      481 gggatgaaga cttcgatttt ttaattcaaa aacttcagaa agaatatgaa gatagattta
      541 atatacttgg aaataacaat attggttctg ccttttctac tgaagcaatt aataaaggta
      601 tatttaatga gtttaaatct gtattttgtt ttctggaaag taatgagaaa gtatataata
      661 tagtttttga tgaaggatat tatgtaactt taaattatac tgggagtaat tctaataata
      721 agatgtatat ggagaaggtg tttaaattta tagaagaaaa caattataag ataattgctg
      781 accctataga aatttacaag atagatatac atgagactgg aattgtagag gagtttgtaa
      841 cggaaattca agttcctata actaaatagc tatatttcaa tggattagat ggtaaataga
      901 atgaaaaaat gttgatttgc caccgatttg tcactgtgtt ataaaatcgg tagcaaatta
      961 tattttaaat tataagatag catgaaaaaa taattgttta gggggttata aatgtgaaat
     1021 gttctaattg tggaagtgta aatattgaaa agggaatttt aacaactggt ggcatatatg
     1081 ctgaaactat tgggttaaaa tatagaggag atagaaaatt agataaagtt ataaataaaa
     1141 cttttccagt acaatctact atatattcag atttatgttt agattgtggc gaaatagtta
     1201 gaacctatat aaaggataat attgatagga attggtgtaa agataagaaa tagaataatt
     1261 tttgaagtat attaatattt gaagttgaat tttattaaag atataagtga aaaagtataa
     1321 atgggaagaa attgttatat ggaagaaact ttgcatatag gggagattag ttttttaata
     1381 taataacatt tttaaatgta gataaaacta aaaagggttc tctagaaaac aatttctatt
     1441 taattatact tacttaccaa taccatcact tgatgtaaac aagattctac tacattttct
     1501 ccgtactcta gtataaaatc atctagctgt gtatctataa aatcacaata atcatataaa
     1561 ctactaatat aagcagttaa catgtgtgta catttatcca tttcatctct aaacatatat
     1621 attactatcc ccctaaaaaa ctttcataaa acaaaaagaa caatatcttt ttggcaactg
     1681 gctgacataa ctcatagttt attttataag cgttagtccc tatagctttg cgtcactaaa
     1741 tttctctagt tttgccgatt tagttttatt ctacaactaa aataatacac taggataagt
     1801 tattgttcaa catacttgtc agaaaattga aatatttaac agtatcttaa caatatgaaa
     1861 tctatttttc actcaagaaa caaactatat atgtagaatg tttaataaaa gttatattta
     1921 gttttgtatt gaagtttttg tattaaaaag tttagttcac aagagctact tggctagata
     1981 atagatgaac aaaaatatct tgaaaaaata tatatgaatg gcaaaaaaca agggatgaat
     2041 tttatataga ctcatcccgt aattatttat atattgacct tgattaaata tattattatt
     2101 tttaagcttc aatttttata ttatatttct aaagtttcta taagaggatt atcgctaaat
     2161 aattttaata aatttgtttt tttatatcct ttaggtaact tagtatatat aactacttct
     2221 aaccaatccc catcaaggcg tttaatattc atgtttataa tttcaatatt agatttttta
     2281 aatttacctt caatatattc tatggaatca gtgctgttag aaattttaag agaaacttgc
     2341 tctgctgtag ggagtttcat ccatttccta tctctatgaa gtgttatctg tacaataaca
     2401 ataatgatac ttgaaacaat accaactaag tataatccag agccaatggc aagacctgta
     2461 ccggctgttg cccatatacc agcagctgta gtaagaccac ttactgattg atttctaata
     2521 aatattaatc cagcacctaa aaaacctata ccactgacaa cttgtgctgc aacgcgggct
     2581 gggtctagac caatacctgg agtacccact atatcattaa atccatactt ggaaacaatg
     2641 attataagtg ctgaagctaa tgccactata aaatgagtgc gtattccagc ttcttttagt
     2701 ctattctttc tctcatatcc aattaaagcc ccacagaaac cagcagtaat gattcttgct
     2761 atatattcaa attgaatcaa tatatgatgg ttcatttatc tagcaccccc ttctatataa
     2821 tattatatat actataatac tacatttttt atgtgaatat agtaaagttt aaaatttata
     2881 taatagttat atatgaatat aaaatgtata aacgtatatt atgtatataa aaatgataga
     2941 aagcatctaa ttttaaatga ttaaagagca agcttaaata aatcaaactt gctcttttaa
     3001 catagtgtat gtttttattt gtgatagtta gataaaaatt tatctgtgtt ttttacatct
     3061 gcaaaactgt tctgtagtga tgccatgaat acttgatgta cagtatttgc attaatttct
     3121 atattattcc aagataaatt cttcgttgta catgcatcgc ttattagagt tatgtcgtat
     3181 cgaagtttct ttgctgttct tacacttgta tcaatacaca tatggctcat cattccacag
     3241 attactagat tagtaatatt ttttttctca aggatacttt gcaaatctgt ttcaaagaaa
     3301 ctatctggtg tgtgtttaat tataatttct tcattatcta gtggaaaaac aattttattt
     3361 atttctgaac catgagtatt aggaagaaaa aaagaagctg ttttatctgt tgatatatgc
     3421 tgtatatgaa taactggtaa attgtttttt ctaaaaaaca gtagtatttt tttagcattt
     3481 tcagcagttt cttctgattg aaataattca cattttccat ttttaaaata atcattttga
     3541 atatcaatca atattaaagc tatagacatg attatacctc ctatttattt ttattataat
     3601 actattgtaa aaaatgtaaa ttacttactt tttggtatgt ataggattac ttattgtagc
     3661 aaattccttt attatctaga atttcttgtt gaccatgttc aagcatatat tcgattccta
     3721 tttcttgcat tatttccact gcttttaaca tttttttacc acgattagta agaaaatatt
     3781 caacatgtaa tggatatcca gcagaactta ttttatcaat aatcccaaaa tcacgtaact
     3841 cttttaattg ttgaatcaac attttttgat ttattccata aatattatgt tgcaattcag
     3901 aaaatgtttt attagattgt cttaattcaa atataaggat agttttccat tttcctttga
     3961 ttatgtcatg tacaatttca agaggacatg tatattcagc tcttattttc atagcatatc
     4021 acccccagta aaatgataat aaattatatc acttgtcttg tgatatgaca aatatatatg
     4081 aactgtatat aaattttatg ttttattaag ttctaaattt cttctattgt agttcatatt
     4141 tagtacataa ataattagta agatataagt aaataaatat ataacatgac tatagaaatt
     4201 taactttaaa ataacttatg ataatttaga ggaggaatat atatgaatat atcaagaaaa
     4261 gctatgaaaa taatagaatt agcacaaaaa attgcaaata agagaggtat ttcagtagaa
     4321 gaagcttgga gtgaagcagt aacagaatat aaaaataaat acgaacatat tgcatagata
     4381 tttaaaaatt tagaatattt ggtagaatat aatgatttta aatgatacta taaattataa
     4441 ggtatatcat tattaacata gaaggaggat atctgcttga ttgtaaaaat agataatact
     4501 ttagaaaaag aattttggca atatgtatca catgaagaaa gtttaaattt attcataatt
     4561 ggatacgttg agaattatgg atttagctct caatatcaag atatatggtc tcaggtagaa
     4621 gatggaaata taacctctat aatattaaaa aataagtcaa cacttataat ttatagtttt
     4681 aaaaataatt ttaatatagg agaaatgaag aatcatataa aagatttaga tgtagaaagc
     4741 ataagtggaa aaaaatgtgt aatagataga ctaataagta aatacaaaga tttttatgaa
     4801 aaactagata ataaattttg tgtattaaaa gaaataaaag aaatagattt ttccaacatg
     4861 aaggagtata aaatagaaaa tgctcaggaa aaagatattg atgaaatagg aaaactttta
     4921 aataggtcag actataaagt aagtaaaaat tacatagaag aaagaaaagt tcatttaaaa
     4981 gagggaaatg taagagcata ttttattaga aatgatgata ctatgattag cactgtgtca
     5041 actggtatgg aaactagttt tttggcaatg gtggtctctg taagtacaga taaaagatat
     5101 aggggaaaag gacttgcaag ttatatggtt tacaatttga gtaaagaatt gttgttagaa
     5161 ggaaaagttc catgtctttt ttataataat gatgtagcag gtaaaatata tcataatata
     5221 ggatacaaag agataaatga atggacaatt ctttttaaat agctataaaa aataaaaaac
     5281 tctatcgaga attaaagtat ttataaataa ctatattttt actataattc atgatagagt
     5341 ttttttaaat ttatgatact attttttatt atttaaataa tcaagtgttt taagtttata
     5401 accttctttt tcccaatgtg taagaacctc atctaatatc tcagtatttg ttgatgaatt
     5461 tggatgcagt aaaactattg caccaggatg agttcttgaa taaattttgt ttttggcaaa
     5521 ttcatgagta ggttgtttct tttcatacca atctacataa gcaaaactcc aaaatattga
     5581 tttatatcct aaatcttgtg tatattttaa agattgttcg ctaaatttac ccattggagg
     5641 tctaaaatac ttaggcattt cttttccagt aacatctttg tatgcttttt ctacacttgt
     5701 aatttcttct ttgaattttt caaaatctgt aatacctgcc attgatggat ggctttttga
     5761 atgattacaa actaaatgtc cttctttttc catacgcttt attaactctg gattgctttt
     5821 tatataactt tcaactacga aaaattgagc tttagcattg tgttttttaa gcgtatctaa
     5881 taattttgga gtgtttccac tttcatatcc agcatcaaat gatagatata taactttttc
     5941 attgggattt cctacataat aagctccgta ttttttaaaa aaatcagctt cttttatagg
     6001 tgatggctgt ttaccatctt ccctagggtt aaaataccaa tcatattcat gtgtgtcgag
     6061 tgttggacta gaaatttgag tttttgtttt atctaatgat ttaaaattta tactagcaat
     6121 tccaaaaagt attagagcaa atgctcctat cataatgtat ttttttaggc tatctttttt
     6181 cacaatttca cctcttattt aaaatttaaa gtatatttct tataatatag tttgaatatt
     6241 tatagttaaa aatattcaca tatacaaata ataaatgaaa gaagtttact gtaagatata
     6301 aagttgatat taattatctt aaataaagtg tatataaatg ataaaatata aagagaaatg
     6361 attgtaaata agttaggctg gtttaacttg aagggatata aaaatggata atataataag
     6421 tgtattgaat gatgttgttt ttttagatat agaagttagt ggattagatt gtttaaattc
     6481 agaaatttta gaagttggag ctgtcaaggt taaagactgg aaaatatata cctatgaatc
     6541 attaataaaa aacaaatttg aggtacctat agaagtattc tcagtatgta aaaatttaga
     6601 taaaaatgat ttagaaatag ctaataaaat agaactggtg gaagatagac ttgtaaattt
     6661 tgtagaagat agtttcatta tatgtcatga tttaagtctg aaaaaaaagt tttttgagta
     6721 tcatatgcca aagttaaaaa ataaatttat tgatttaatt gaattagcag ttattttaga
     6781 accatatcat aaagattata gcttagaata cttaaaaaat actttaacaa attgtaattc
     6841 taaagtggaa aatagagctt tatcagatgc tattgatata ataaatattg taaattgcct
     6901 tttagtaaaa tttaataact atgaaaaaac tactttggag ccattgagtt ttaaaataaa
     6961 ttcatatctt aaaaaattta atcttccaac atgggaatgg agcaaatttt tagaagaggc
     7021 aaattatgat ttatcaaata atataaatat aaaaaaagaa tataatatat ttgactcaaa
     7081 agaagaaaag aagaaggaaa gagaaacctt aaaaatttta aatgaagaag aaaaaaatta
     7141 tgaagaatta ttaaaataca aaactatttg ggagaataaa gaaggattta catatgaata
     7201 tagacctggt caatatgagc ttactaaaac aataagagag ttgtttagaa atagcgaaga
     7261 tgaagaaaaa attgcatgta tagaggcacc aactggtata ggtaaaagtg taggatattt
     7321 attgcctgca atattagagg ctagaattaa taaaaaaaga ttattaattt ctactgacac
     7381 aaaggaattg cagatacaat taataaataa agatattcca aatgtgttag attctttggg
     7441 tctaaatgga aaagtaagtt acggatatat aaagggaaaa aataactata tttgtataga
     7501 tagactggaa gcatatatag atgattatga gtctcaaaac cctactaaag gagaaatttt
     7561 atctttaata tttttgaaga gattagttga aggtggcaag tatggagata tagaagaaat
     7621 aaattattta gtgtttgaca acttcaagga aataagtact cacttaagaa atgtaagttg
     7681 tgacccaaat atgtgcagac ctaaaaaatg caaaaaagat tgtttatata aaaacagaat
     7741 agaagaatta aaagaagaac atataacagt agtaaatcat tcactattag ctaaatggcc
     7801 gtataaggat gaaaaacctt tagaaaatat aatagtagat gaagcacata atttgactga
     7861 aaaaggatat gatttctttt caagtattat aaattcaaag tctttaaggt acttattaca
     7921 agaaatttat ccttacgagt ttattcaaaa tagtagcttt atatataaaa aatatagtag
     7981 aaatatgaga aaaataaagg cttttgataa attttataat gtattaaaaa ttggaagaga
     8041 agataaacaa aaaattgcta gaagtataaa tttcattatt gaagagatag attcaatatt
     8101 aaagtttggt aattgtaatg aatataataa tgtaagtaat tataatttaa gatgggaact
     8161 gaatttgcaa atagatgaaa ttgtgggaaa actaaaaaaa gatggtatag atactgaaat
     8221 aagttataga gcctatagtg aaaagataaa gttgagttgt gagaaaataa taaaaaattt
     8281 agtttcaata ataataataa tttataggaa tattgatgat gatagtatag ataaagaagc
     8341 agatatatat aaatttggaa aggcaaagac aagagattta gaggatataa agattatatt
     8401 tgaaatcttt ttagagtatg atgaaaagga tgattatgca aggatagttg aaattgataa
     8461 gaattataat gactttgaat ttcgagtagt accattaaaa atagcagatt tatttgaaga
     8521 aaatatttta tctcaattag aaaaaggaat atttttatca gcaactttga gtttatctga
     8581 aagtatgtct tattttaaaa atacattagg aatagacaga gttaagaatg tggaaaagat
     8641 tatagaacca atttttgatt ataaaaatag agtctctgta gttggatttt ctgatatttg
     8701 tgagtacaga aattctgaat ttccaaatga gatgagtaaa attatatcta atatatcaaa
     8761 gattacagaa ggtcatacat tagctctttt taatagtaaa gatagacaag aaaaaactta
     8821 tgaaatattg aaaaaatact tacatagttt taacttagaa atttatgctg ataaaaaggg
     8881 cattagacac cttaacgatt taaatagaaa atgtgtagta cttggctcaa aaggatgttt
     8941 tgaaggcgta gacattccag gggatggttt ggtttgtgta actttagaca agcttccgaa
     9001 tctaaatcct aaagaccctt tgtattttac tataatgaag aagtatggta tagactacta
     9061 tactataaat tatcctcaaa tgaccataaa ggtaaagcaa gctatgggga gaattttaag
     9121 aagcaaatat gactatggat gttttgtgat ttttgatgta ggaactaata taagtgtgtt
     9181 aaagagatta gagaaagatt tacacgattg taaaatttca aaggttaata gcaatgagtt
     9241 ttatacttat ataaggcgac atttaaataa atctagaagt ttaatactaa aatctgttat
     9301 atttgatact ataaaagcgt tgaatgttga tgctaaaatg gataacaatg atgttgataa
     9361 ggatattata aaaaaagata taaatgaaaa tataagacaa agagctgtaa aaggagaagt
     9421 gtaccatatt gatattataa aaaaagatat gaaagtaaaa tattttgata gaaactatct
     9481 tataaattta gatatattta tgagagaaga agataaaaat taagagtggg agtgatatat
     9541 ttggcaaaac aaaagtttta tgctgtgaaa aaaggtaaaa atataggtgt atataataca
     9601 tgggatgagt gcaaaaaaca agtaaatgga ttctcaggag ctgaatataa gagtttttct
     9661 acttttcaag aagctaaaga atatattgat gggtcagaaa aattgagttt tcaagaagat
     9721 aaggagttta tagaagcata tgtagatgga agttatgaac attcagtaaa aatgtatggt
     9781 tcgggtgttg tgattttaaa aaataatgaa gttataaaaa cttatagtga aaaaggaaaa
     9841 gagaaaactt tagtaagtat gagaaatgta gctggagaga tagaggcttc taaaattgca
     9901 atgcagtact gtattgacaa taatgttcaa aacttgattt tatactttga ctatgaaggg
     9961 attgaaaagt ggtgtactgg agtttggaaa acaaataaag aaggaactat agcatataaa
    10021 aatttctatg acagtattaa gaataagctt aatgtaaaat ttacaaaggt taaagctcat
    10081 tctggaaata agtataatga ggaagctgat aaattagcta aaaaagctat tggtgtataa
    10141 taaaaaaata tttatacata attacatggt ataagagtat atctaaattc ctactttata
    10201 attaaacaac ttgtaattaa ataaattttg attaaaaaag cagattgtat tatgaaaaaa
    10261 taagtatcta atttataaac aattaataat agtattaatt agatacaact tatttaatat
    10321 aataaaatct gctttttata taactataaa attttactac attctattag aattgcccat
    10381 aaataccttc aagttcttct tttaatactt cagggtcatt tataccaata gacatggttc
    10441 tagccagtat atttgcttgg tcataagttg atgtccatga gaatacacca cctagacaat
    10501 ttctaagaac atattgagtt tttaagaata ttgataaagc atcatcatat gacattgcaa
    10561 aatctccatc cttaactaaa tacggaactt gagcagtatt atcaaatcta tattcatatc
    10621 catttttatt tatataatcc cttctaagct catcataagt tcttgttata gtagcaccta
    10681 atcttccata aaatggtata ccaagaagga ttttttcaga aggcattcca gcattctcaa
    10741 gatttctcac cattgcatca acactatatc ctggcaaaga taagtctgaa tcaaaaagat
    10801 ttgcttgatg ttttctacca tttgggcctg tttcacctgc tgtaaaatca taactcataa
    10861 gattaaaata atctattata ggagctattt tatctatttc agcacttgaa tttatatatc
    10921 ctctatctcc tgttccagct acactaagcc atttatcatc tcctataaca tctcttatgg
    10981 cagttagtaa gagtgtaaaa ttttctctat cttgaggtct tgatgttatt ccagatgcac
    11041 tacttccagg atattcccag tctatatcta ttccatctaa agcatattca tttatcatct
    11101 gattgacctg tcttgcaaaa ttatatctgg atgtaggtgt taatgctgca tcagagaatc
    11161 cttcagctcc ccaacctcca atagctacaa ttacttttaa gctaggtttt tcacctttaa
    11221 gagcaagtaa ttgatttaaa aatctaggat aaggaataaa caaatttcca gattggtcta
    11281 tctcagcaaa agcaaatata actgcatcta atagttgagc atctacatta gttggatttc
    11341 caagaacata ttctgctaca attggattta tatctggaca gtttgcattt tcaactattg
    11401 gattgtatgg gtctgttatt tgtggtctag ctagtttttt tgaatctttt aaagtcttca
    11461 tagctatttt tttagtattt ttttcactag tataatcgta gtttttataa ttatgtttat
    11521 agaaatcttc ataactacta tcttctttgt tatagctaga atttattttg tcacaagatt
    11581 catctttatc atagttaaaa ttcatttcat cacaagactt atctttaaac ttatcttgtt
    11641 tggagttgtg aactttgtta gtatttttag tatgtttttt ctcatggttg caatcatctt
    11701 taatatcagt cttgtgttct tgtttacagt caagagcttt gttcaggtag tcatgatgtt
    11761 catgttcaca gttacattca ttttcaatgt ttttagtatg ttcttttttc cagctacaag
    11821 tattatcaat tttcttgctc acttcttcat cattataatt atctggtaca aaacataagt
    11881 accagtccat acatgaatat ttcttattac aactgttaac tctatttttt aattccttat
    11941 agtttttagg ataaggtaaa atcactggca ttccaggaaa ccagtttgca ggagtaacta
    12001 tattatctct atcactagtc tgaagtgcat ctactattct aagtatttct ggaatatttc
    12061 ttcctgtagt tagtggataa taaagaatcg ttcttagaat ttgattatta tctataataa
    12121 atacagagcg aacagttgat gtatcactca ttggttttga tatcatgccg tataacttgg
    12181 caattctcat atctctatct tctataatag gaaatggaat ttctacacct gtaagtaaag
    12241 aaatattata catccaagct aaatgtgaac tgttactatc aacacttaga ccaattagtt
    12301 ctgtatttct ttttttaaat tcgtcataat atttagcaaa acataaaaat tctgtagtac
    12361 aaactggtgt aaaatcacca ggatgtgaaa ataaaacaat ccaattaccc ttatagtcag
    12421 agagtctaat aggaccattt gttgtattgg ctttaaaatc aggagccttt gaccctaaac
    12481 ttggcaaatt tggcatgtaa atcacccctc aataaattat catttatttt attctattaa
    12541 ttaatacata ttagtgttac tattttatta aaagttaagt aatccttatg tcaaaaaaag
    12601 atatttatta aaaagattta atattttcct aatataaatg acaatagtaa ttgctatttt
    12661 tttactaatt tggtatagta atggataagg ggtagtgtca attttagtga ttatacgagg
    12721 tgagtggatt gaaaaaaatt ctaagaattt tagttatctc catgatgata tgttttgcaa
    12781 tgacttttgc attagtgtta ataaattcag ttatgggagt acatatagga ttggattatt
    12841 actttttgat gggaagtgtg cttacagttt gtttaacaat tggaattgtt ttatgcaatg
    12901 ctagaaaaat atttgatagc aaagctatac acaagacaag aacgaaaact gtcagtaata
    12961 ataaaaaaca aaagagtcat tctagggata taaaaagaaa gatatcataa tttatgtaaa
    13021 ggaaatattt atgtataaaa taatgattga taggtaaaat aaaaacacga tttaacttgt
    13081 gtttttattt tttttcaaga attatgatat aataaccaat aagaaattta gtttaccata
    13141 aaatataaag attattaaat ggagagggaa ctataatgaa ttttagagat ttatttgaga
    13201 aggctgtaga ttttattgat gaaaaaagga aatctatagt tgcgatttca ttatcaacat
    13261 taggtgtaat tgctttgata atagtatttt tcttaagtag taacgagttt agtgtaggaa
    13321 atgaagctaa tgagttgtta aagattatag agaaaagaca gtattctata gctgttgatt
    13381 actacacaag tatagaaaaa aagttttctg attcaaaaat ggaaaggttt aataagtctg
    13441 tttctaaaaa aataaataaa ttgctattaa atagtggtga taaatattta gatggagaca
    13501 taagtcaaga aagttttatt ggattaataa ataccgtgaa agaattaaac aaaataagta
    13561 tagatgtgga tgacttatta gctcaagctg atagagttag agaaatgtat aaagaagaaa
    13621 atactactta tgatattgcc attaactaca taagtactgt ttcaatatta aatggtatgg
    13681 gtagtaacct agatgtatac aaacagaata tagaaacaat caaagagtct agagatgttt
    13741 atgactcagc agtaaaagac caaaaagcat ataagtatta tgaagctatt gaaaagtata
    13801 ataaagtatt aaaagaagat gaaaagtatt acagtatggc tcaaaatggg aaagaacaat
    13861 gtattgaaga aatgtatgat tactatataa gtagagctga agaggcaaat acttctggag
    13921 attatgaaag agcattacaa tatattgagt atttaaaaga cgattattca gatgatgaaa
    13981 aggtacagag tttagaaaaa aaatataaaa agaatttatc attgtatact ttaacatctg
    14041 atgacattat aaatgtaata gctaaaaaaa gtggcaaaga caaggcaaat ctatctataa
    14101 attcacttcc acaaatgata aaaaataata aatactatta tgctgaagta tatgagtatg
    14161 ataaattaat aaatgaagtt ttggtaagtg caaaaactaa agatttatat tcatataaag
    14221 atggtaaaaa agattacaaa gtagattatg gtgatggata ctttagaata ttagaagatg
    14281 gaagttatca atttggtatt acaaaagata aagctaagtt tgtgttaact aatactcttg
    14341 atgaaaaaga aaataagtat aaaaagattg agatattaga tatagagaaa gcagatagat
    14401 atgtaaaaag taaaaagagt ttagaagaat tgtttggaaa atataaaaat atttattatt
    14461 atgctgtagt aaataaaggc ttatttagag gcaaagaagt ttatgctatt aatatttaca
    14521 atgaaaaaat atatgccata tcagaaaatg gactaaatga atattaatta ataatattaa
    14581 attaatatat aattaaaaag tttttggggt tttaaggcgc attatgtagt aatataataa
    14641 tagggatata taaataattt tattgtaagg agaagtatgg gatgagtaat gtgaacaaaa
    14701 agctagttat agctattgga gctgtagcat tagtagtaat aatttttata ggcataatgt
    14761 gcatgcaatt taaaggggag aaaatagcaa aaaacacata tgttaatgga gtaaatattg
    14821 gaaaattaac taaatcgcaa gcaaaacagg aattagctaa aaaatacaaa ttagagaatg
    14881 ttgattttaa ttacaatgat aaaagttgga aagtaaagag caaagattta aatttatcta
    14941 ctgacctgga taaaacagta gaaaacgcat ataatttaaa tagaaagagt gctttctttg
    15001 gcaatctatc aaaaactatt tcagcaaatt ttggcaaaaa aagcaactta gtagttgcga
    15061 taaattatga taaaaataaa ttaaaagcag aaatggaaaa gatagctaaa gaaatagatg
    15121 tagacgtaaa agatgctaca cttgatataa gtggtgaaaa agtcaaggtt ataccagatt
    15181 ctgatggtct taaaatggat atttctaaga gtatggagaa ttttgataat caaactaaaa
    15241 aaggtaacta taaaaatgag ttagtagtaa aagctactcc agcaaaagta aaaaaagagc
    15301 aacttgctaa tatagataca aatttaggta cgtattcaac aacatttaaa acttctcaaa
    15361 taaatagaag tataaacata aaattagcaa cagataatat aagtaatgta ttacttatgc
    15421 caggagaaac tttctcattt aataaacata cagggaaaag aagtaaggaa aatggatata
    15481 aaagtgcacc tgtaataatg gaaggtgaga tggaagaaga ttatggtgga ggagtttgtc
    15541 aagtttcatc tacgttatac aattctgtgt tatacgcagg tttagaaatt gttaatgtaa
    15601 aaaatcatac tataccatca agttatgttc caaaaggaag agatgcaact gttgcagata
    15661 gtggaataga tttcttattt aaaaataatc taaagcatcc agtttacata aaaaattacg
    15721 tttcaggaaa tcaaattgtt tgcaatatat atggaagtgc agaagataaa caaaatataa
    15781 caatatcaac aaaattagat ggcgtttcac aaactacaat gaaaagagtt aatgacccta
    15841 caatgcctaa aggtaaagaa aaagtagaca aaagtggaag aaatgcatat tctgtatcaa
    15901 catatagaac atttaatgat gcaaatggta aaaaaattaa aactgaaaaa atagctaatt
    15961 catattatcc taagaaggaa ggtattatat tagttgggac tatggaacca aaaccagagg
    16021 aaaaaccaaa tacagacgaa aataagaata atcaaaatcc aaataatcaa caaaaaccag
    16081 aaacaccacc aactgataat aagcctaatg agacacaacc acaaccgcaa gcttaaagtt
    16141 ctcaagaata ggatttatgt agagtaataa aaaatataaa tctaaatagg agggattact
    16201 ttgaatttaa ataatataac gaattacgtt caaagtattt gcgagaatat ttctaatgtc
    16261 ttggatgtag atgttacttt agttacaaaa gatttaacta ggatagcagg tacaggaata
    16321 ttcagaggta aaataggaga gacaatatca gagaagtcag tgtactctct tgttttaaaa
    16381 gatggaaaat cctatgttat aaataaagaa atagaagaga actgtaataa atgtgcaaat
    16441 agagaaaatt gtaaagagtt agctgatata tgtactcccg ttaaaatggg agagcatata
    16501 cttggaatct tgggtatagc tgcgtttaat gaagaacaaa aagaaaagat actttcaaaa
    16561 gataaggagt taactgcttt tgtaaggagt atgtctgatt taatatcttt taaactagat
    16621 gaactttata aacaagaagt taagactata gatgaccttg agagggaagc tatagaaaat
    16681 gccataaaga aatatggaag taatacagaa ggtatgaaaa aagtagctga ggcattaaat
    16741 ataggtatag ctacactcta tagaaaagta aaaaaattca acattaaaag ttaatagtat
    16801 tgaaaaagag gtaacctttt tatgaaaaga agaggattac cttttttatt atgagaatgg
    16861 tggaaaaata tgtatagttt tgacttgaca tattaaatat gttatcttaa atatatattt
    16921 tagagtggag gtgagtatta ttttagttaa agatataaaa caagacatag aaagcatgcc
    16981 tagaaaaatt ttagatatat ctttgataga agataataaa agtgatttga ctttttttaa
    17041 tatagaagaa gaagacggtg atgaaacatt aaaagcatta aaacagagtt atgtgactac
    17101 aaattataaa gaagaaactc tattaaactt gcaaggagct ttaaattcaa agtcagatac
    17161 aacgtctaag gagttaaaat tagaatcata taaaagatat aacaatgcat tggatttagc
    17221 atataaaaat tatataacta gtgccattaa tatggttaat aaagctctgg agataaatcc
    17281 taaagacatt gatatactaa atcttagagg tcttttaaaa ttattaaaat gtgattttgc
    17341 taaatcattt gagagttttt atactgcttt atgttatgaa aataatcata tatctagaaa
    17401 atatgtaaat ctaatttcat ctgaagattt taaaactttt ttaggaagat acaatcattc
    17461 tataagattt ataaatgaag aattaaatta tgagtcaata catattttag agaacataat
    17521 tgaagaagaa cctgaactaa tagagccata tgtaatttta tctttgttat atgataaact
    17581 aggtaatgta aaaaagagag aaggatattt agacaaactt aaagagctag ataaagataa
    17641 tcctgtattt gaaaaaaatg aagaagaaaa agaagataca agtaaaaagg aagaaataaa
    17701 aaaagtaaaa agaaagaaaa gtattttgcc atatattata gttggctgtg ttttaatagg
    17761 tatggggata tatttgattc aaagcaaaaa gagaatagaa aacttgaata accaaataag
    17821 tagtaaagag gaaaagttaa gtgaaactga taaaaagtta ggagaaacga gtaaaaaatt
    17881 agaaaaaacg aataaggaat taaatgaggt taaaaataaa gaaccagaaa gagagataac
    17941 tgtagctgat gaggaagatt tatattatca agctctaaat ttaaagaaaa acaaagaata
    18001 tgaaaaagcc atagataatt ttaaaagtgt agtttcttct ggtaaaacta aaaaatatat
    18061 ttctgagtca atatatcaat tagcaataac aaataaatta ttaggaaata aggatgaagc
    18121 tataaaatat tacaaaaaat atataaatac ttatactaaa aatgaccagt attatgatga
    18181 ttcatattac gagttgggta tgctttatta tgataatggc gatttaaaaa atgcacaaca
    18241 gactttttat agtttgagaa gtgaagttcc agacagtatg tataacaatt caaaaataaa
    18301 agaaatttta agtgaaaaat aggtttatat agttaagttt tttgaaacaa ccaagtatgt
    18361 attttattaa attaaaataa aaataaattt aaaaagagat tgtgtcaaat tataactttc
    18421 tattttaaag aaagatattt gcttgttgat aaaactacaa atatatgatt tgaagcaatc
    18481 tctttaattt ttggaggtgg aaaatgaaag ttttcaatca attggtaata attttatcta
    18541 tctgggtaat aggagagtat ataagttcct ttatacaagg aattatactt attcctggga
    18601 gtatagttgg tatgctatta ttatttctac tactacaatt taaagtttta gacttgagta
    18661 gtatagaaaa tgtaagtgga tttttcctcg ataatatggc tatatttttt attccagcag
    18721 gtgtatcact tataaagtca ttagacctaa taagggaaaa tgtattagtg cttttattag
    18781 tcatatgttt aagtacacta atagtaatgt atactactgg aattattgtg gagaaaatga
    18841 taaaaaagaa ggaagaagga caaaaaaatg tataatgtat tacagacacc agtatttggt
    18901 ataatagtaa caatagtttt tttcaattta ggaagataca ttcaacgaaa aacaagtaat
    18961 cctatatgta accctttatt aattgcaatc gtaggaataa tattgttttt aagcatatct
    19021 aaaatccctt atgagaatta caagatagga gctgatagtc taaatttctt tttaggtcct
    19081 gtcacagtag tacttgcagt tccactttat aaacaatttg aattatttaa aaagcatatg
    19141 tttgagatta ttgtaggaat aggttgtggt atagtgattt cttttgtatc tgtattgata
    19201 attggcaaaa tagctaattc agatgtaagt ataataaatt cactaattcc aaaatctata
    19261 acaacaccaa tgggaatatc tttagcaaat tctttaaatg gtatagaatc aataacagtt
    19321 gtagccataa tttttacagg tatttttggt ggtatggttg cttctacagt attcaaactg
    19381 gggaatataa atcatccagt tgctaaagga attgctcttg gaacatctgc acatgcttta
    19441 ggaactacaa aggcttttga actgggagaa atagaaggag ctatgagtgg tgtttctatt
    19501 ggagtctctg gtacaattac agtaatacta attccaatta taatgaattt tatataaaaa
    19561 ataaaattaa gacgagtacc ttgaatactt aaaacaaaat aaaataaaaa aaatttaaaa
    19621 taaaaactga atgaaaaaaa tgcaaataat gaaataaaaa aatcatgtac tgaaattata
    19681 aaaagaaaaa tttcatttag gaattaatta taaagagaca attactaaac ttgaatttgc
    19741 taaaataaaa taaattactg cgaaaataga tatttagaaa aaaatactat aaatttacta
    19801 tattgcaagt taaataataa gcagtatcat attatgctag aaaaagtttc atttaaggca
    19861 taaatgagat gatttgaacg tttttaaaga ttagaatata aaagattgca ctagacatat
    19921 atgaaaaaga taatataata gatgatgtaa tagatgacag ttcaagaaat tataaaaaaa
    19981 atttatttga aatattatct attacatcag gaataatata tgtaagagta agcttttaaa
    20041 attctaaaga tttagaaaga ttgaagggag aaagaaatga gtgaagttaa gaatacccta
    20101 caaaaacatc cagttttaag atattcaaca atgctatttg ggatgttgat gacatctata
    20161 ggaataaatg ggttcttaag accagcacat ctacttagtg gaggtgctac aggtatagca
    20221 acatctataa actatttgac aaatataaat gtaggtttat taacattttt aatcaatatt
    20281 ccaatattta tcttagggtt catatacctt gaaaaagagt tttgtatttc aagtttggta
    20341 aatatgattg tattttcatt attattagga gcaactcagg aaataagtaa tattatacct
    20401 atacatgata tattgcttca aagtgtatat ggtggtatat tatctggttt aggagtcgga
    20461 gttgtattta gaactaggtc ttctcaaggt ggtacagata taatagctgc aatactaaaa
    20521 attaagaaga atatagaaat gaaagacact gctttagcaa taaatggctt gatagtactt
    20581 acaggaagtt ttttatttgg gttggattta gcattataca ctcttatagg tctatttcta
    20641 aatgcgtatt caatgagttt tattaaagat gcaatgaatt accaaaaatc tgttatggta
    20701 atgtcaaatg aagtcgattt aatagcagaa gatataatga agagtttagt tagaggtgta
    20761 acttttttag atgcagaagg tgcatacact catcaaaaaa agaagattat atatacaata
    20821 gtttcatcaa atgagatacc aaagataaaa gatatagctt tgaagtatga taaaaaggcc
    20881 tttatatctg taaatgatgt tacagaagta aaaggaagag ggtttaaagc aaaagattta
    20941 tagaaaattt tacaagctgg gagtttataa tgtaaatttg tatattatat ctctcagctt
    21001 atttaaattt agtatacgaa aataaaggtt ttattaatga tttgtgtatc actttaaaag
    21061 atatcatttt aaaattaaaa ttgaaaaaat tacttaatat gtagaaaaca tgaccactat
    21121 taggatataa taaatttaat agatttagta aaatatctag gaataacgtt ggaggaatgg
    21181 cattatgaat ttatctaaag aatatgttaa tttgtatgat aattatttga aatatataaa
    21241 tgaagtaaac aaagagatta gaagtataaa gaacatgaag aaacgtgttt taaaaagggg
    21301 cgattttgct ccggaaaatg aagtattaga attggaagga aaaacaatac gcgatataga
    21361 tgatgtcgat acaaaaaaac caaaaaataa aatctataaa ttttcaaatg gggatgtata
    21421 tgtaggaaaa ttcttagagg gaaagatgga ggggaaaggc tcatatactt tttttgtaga
    21481 tgaaggaaca gttatggagt acataggaga atttaaagaa gataaaaaaa atggaaaagg
    21541 aaactttata ttttcaaatg gaaatgaata tataggttat tttgaggaag atatgatgag
    21601 cggtattgga aatatgttat atagtagtaa agatgaatat attggaactt ggaaaaacgg
    21661 aaaaaaagat ggaaaaggta tttataagtg gagtgatgga tgtatatatg caggtgaatt
    21721 taaaggtgga aagatggaag ggtacggtat ttgctataat agtaaagggg aagtacttta
    21781 tgaaggagaa tggaagaata atttgattca tggtaaggga acttatatat gggaaaaagg
    21841 taaaaagtat ataggtgaat ttatgcatgg taaaaaacat gggcaaggaa ctttttatct
    21901 aaataatgaa ttggtttatg aagggacttg gaaatttgat aagccatcta tatttgatag
    21961 aacattagat gaaatatttt ccttgagact gtagctttat ctaattagta gataaagctt
    22021 ttttctaata cttactgtaa ataggtatga atgatattga ttagtataca ctatcaatat
    22081 taatttaata aatgcaatat taaaaaattt tgattgattt taagaaggag agatatattg
    22141 aagagattaa ttgctttagt gttagtagta cttattgttt tattttatag tcctgaatta
    22201 ttaaaagaag gatatgattt aaaagaaaag ttttttcaaa gtgaaatatg gaatgaattt
    22261 ttagacaaaa taaatataaa tgataattct aagaaagata aaaaagaaaa aagtcaaaat
    22321 ttacaatcaa ataatactga aggggagaac ttaggagagt cagatataaa gaattttgaa
    22381 aaaataagct taggagataa tttaagctat gttttatcaa gtattggtaa acctggtaga
    22441 attgatatta gtgaatatgg ttttgattgg tatgtatata atcaatatgg aaaagaattt
    22501 gcaatggttg gacttgaaaa tgatgaagta gtagcacttt atagtaattc tattaattca
    22561 tgtgaaaatc aagatataaa actaaatcaa gatagacaaa cagtgagaac taagattaca
    22621 ccattaaaat ataagagaaa aggaaatacc agatatataa taaactcaga aaatcaatat
    22681 gatataataa gtaaggaagg taagtatatt acaattttct acgatattca tgaagaaaat
    22741 agagtttgtt catacttaat aattgataaa agcactgaag atgagtttaa aaatttatat
    22801 ccagatgata gcgaagaact taaaaaatgt tttgaacttg aagttataga ccttgtaaat
    22861 agtgttagaa atcaaagagg gttaaactca ttgagataca gtgaacaggc aactttgagt
    22921 tcaagaaaac atagtgaaga tatgagagat aataattttt ttgaccatgt aaataagaag
    22981 aatgaaactc catttgacag aatgaaaaga gaaggtatag tatatacttc agctggagaa
    23041 aacatagcag caggacagat taatgcaata tatgcgcatg aagcttggat gaactctgaa
    23101 ggacatagaa aaaatatttt ggggaactat aataatattg gagttggagc aatatttgga
    23161 ggaagctata aaacatatta cactcaaaat ttttataaat aactaaaata tttgaaagga
    23221 agttaaaaaa tgacatcatg gaaaaaaaag acagtatata agtgcttaat agcagtagtc
    23281 ttgttttgtg gaatagtgtt aatatctaat tttagtaaag tttctgcttt gatgatggat
    23341 actaatggaa atgttttgat taaacatggt tcaagggaaa aaaagctaat agcaattacc
    23401 tttgatgatg gtcctcatcc taaagaaact agtcaagttt tagatgtatt aaagaaatac
    23461 aatgtaaaag ctactttttt tatagctgga aaacatgcaa aatggtataa agaaccatta
    23521 gtaagagcaa gtaaagaagg tcacgagata ggaaatcata ctttcaatca tcctgatata
    23581 agtaatttaa gttctagtca gatagaagag gagattgtta agtgtgaaga cattttgaaa
    23641 gaagtaactg gaaaaaaacc gacattattt agaccaccat ttggaagtta tagagaaaaa
    23701 gaattaatag aaattgcgaa gaagcatgac tataaagttg ttttatggac aggtgtagat
    23761 gtgaaagatt ggaaaaatcc aggagctaat agcattgcag ataaaataat taataaagta
    23821 caaaatggag atataatact tttgcatgat tatgcaacta atgatacagt tgaagcttta
    23881 gatatgttta ttccaaaaat gattgaaaaa ggttttaaat ttgtaacagt ttctgagttg
    23941 ataaaataat ttttaggggg gagatttaca gtatgattct tatgatagac aattatgatt
    24001 cttttgtata caacctagtc caatatatag aagaattagg ggaaacagtt gtagtaaaaa
    24061 gaaataatga aatcaaaata agtgacattg aagaactaaa tccagaggta attgtgttat
    24121 ctccaggacc gtgttctcca aaagaagctg gtatttgcat agatattgtt gagcatttta
    24181 aggggaaaaa acctatacta ggaatttgtt taggtcacca aactattgga catgtttttg
    24241 gaggagatat tataaaagca caacagcctg tgcatggaaa agtatatagt atcaaccata
    24301 ctaataaagg agtttttagg ggacttaaaa atcctttaaa tgtcactagg tatcattcac
    24361 taatagttga ctccaataca gttccaaaag aattagaaat tacagcaatt actgataaag
    24421 gggaaataat ggggattagg cataagaaat atttaataga aggagtacag tttcatccag
    24481 aagctatttt gtcagagtat ggtcatgaaa tgcttaaaaa ttttataaca gaagctagag
    24541 aaagggtaca tatgtaatat gataagagag ataaatacta aattaaattc ttttgagata
    24601 tttactattt ttagaaatga acatgacagt tttatattag acagtgctat ggataaagaa
    24661 aaattgggaa gatattcttt tataagtagc caaccattta aggtattaaa gtataaagat
    24721 actgatgaaa atcctttaga agttttaaaa gaagaactac ataagtatag ggttgtgaat
    24781 gatactaatc ttccatttgt aggaggtgct gtgggatatt tatcttatga tttaggtaat
    24841 tacatagaga atttacctag aactgctgta gatgatattg agatgcctga tatgtatttt
    24901 ggattttata atcatgtaat agttatagac catcttgtgc aaaaaactta tatagctact
    24961 ccaaacatag atatcgaatt agaagaaaag ataatagatg atatagaaca aagaatatta
    25021 aaagaagaga aaaaaggtat agatagcata tgttatgaag aaaaagaagt aacacctata
    25081 agacttaaat ctaattttac aaaggaagaa tttaaaaatg cagttcaaag tgttagagag
    25141 tacataagac aaggagatat atatcaagct aatttaacac aaaggttcag tggagaaaca
    25201 gaacttacaa gctttgaatt gtatagggac ttaagaagat ttagcccagc accatttggg
    25261 gcattcttaa actttgaaga tgcacatata ttatcaaatt ctccagaaag atttataaga
    25321 tgtgttaata agagaataga aactagacca ataaaaggta ctcgtccaag agggaaagat
    25381 aaagaagaag atttaagact tcaacaagaa cttagaaata gtgaaaaaga tagagctgaa
    25441 ctacttatga tagtagattt ggaaagaaat gatattggca gaatatctaa aactggaagt
    25501 gttaaagttc cagagctatt tgtaattgaa ccatatgcaa atgtaaatca cttagtttcc
    25561 acagttgtag gtgagttaaa agatgataaa gatgctactg atgtaataaa agcaactttt
    25621 ccagggggat ctataacagg agcacctaag attagagcaa tggaaattat agacgaatta
    25681 gagccaacgc aaagaaatgt atatactggt tcaataggtt atataggttt taatggtgat
    25741 atggatttta atatagcaat tagaacaata atcaaaaatg acaaaaaagt ctactttcaa
    25801 gttggaggag gaatgacttg ggattctgac ccagatgaag aatatcaaga aacacttgat
    25861 aaagctaaat ccataatgaa ggctttgagg ggatattatg aagaataatg taggttttga
    25921 tagtagttta agtaaatttg gaattggttt gtttgaaact attagagtta aaaatggaat
    25981 agcagtagat ttagatatcc atatagaaag aatgctaagt tcaataaatt gtttggattt
    26041 agatataaac tataaaaaag atttcttgat aaatgaaatt gttacatata taaaaaaaga
    26101 aaatgtcatt aataaagcac ttagaattac tgtttttgat gaaggttata atatatctat
    26161 tagggatatt tcatataatc aagagacgta tgagaagggg tttaaattag ctatatctcc
    26221 aatagttagg gggaatagtt taattcatag acataagact actaattatt ttgaaaacat
    26281 atataccaaa aatatcgcaa ataagactgg ttataatgat ggtatatttt taaattcaga
    26341 agatgtaata ttagaatgtt ctatgagcaa tatttttttt ataaaaggag aaagagtgta
    26401 cactcctagt gatagattac cgatattaaa tggaataata aaaaaaagga ttatagagat
    26461 ttgtgacgaa ttacatatag agttgataga aaatgaaatt aatatatcag aaattagtag
    26521 ttttgatttt gtttttgtca caaatagtct tatgggcgct ataaaggtta cagaaattga
    26581 caaaataaaa tttaataaag aaaatgttgt ttttgataaa atagtgaagt tattagaata
    26641 aattaaaata ttaacaattt gttggagaca aaagatgata ggttgtattt atgaagtata
    26701 caatgaagag ataactaaaa taatagaaaa ccaaaacact ttggctgtat atttagtggg
    26761 ttctagtaaa gacgtagatt ttactcttta tgatgtagtg ataaacgata ttgatgtgtt
    26821 cgtttttgta aaagaagggg aaaaacaaga gagaatactg tttaaaaaaa agggaataga
    26881 atttgatgta aattactttt caaaagatgg tgtaaaaaaa ttattatcaa atagggaata
    26941 tttctttgtg aaagaaatga aagatgctaa agtactgttt gatagggaga atatatcaca
    27001 tattataaaa gatataagta gagatattta cttagaagga cctagtaaaa tgtcattaga
    27061 ggaaaagtct tttataaagc aagatattgg agcaaaaata tcgaatttaa aaaataaaga
    27121 aaaatttgat gtttttgaat atcatttttt gacgaattta tatttaaaag atataataat
    27181 tgggtacttt aatataaatg ataaatgggt accaaaagac aaaaaattgc taaaagtttt
    27241 aaaaaaagaa aataatgagc tttttgaact agtgtcaaaa gtaagtgaaa attatgacta
    27301 tcaaagactt ttagatgttt ataattatat ttttaaagaa attgagacaa aagaggttat
    27361 aaaactaatc ttctagtcac aaatcaatgg ggggagttta aatgaataaa gttgataaag
    27421 aaaagataca acatgcagta agagaaatac tagaggcaat tggggaagac ccagatagag
    27481 aaggtctaat cgaaactcca aatagagttg ctaggatgta tgaagagata ttctctggtc
    27541 ttagtgaaga accaagagac catttaaaag ttctttttgc agatgaaaaa catgaagagt
    27601 tagtattggt aaaagatatt ccattttatt catgttgtga gcatcatcta gttcctttct
    27661 ttggaaaagc tcatattgct tatttaccaa aaggaggaag acttacaggg ctttctaaac
    27721 ttgctagagt aatagataca ttggcaaaaa gaccacaatt acaagaaaga atcactaaaa
    27781 atgctgcaga tataataatg gaagaattac aaccttatgg tgtcttagta gtagtagaag
    27841 ctgaacatat gtgcatgaca atgagaggcg taaaaaaacc aggttcaaaa actgttacat
    27901 cagcagtgag aggaatattt gaaaaggaca ttgcgtctag agccgaggct atgagtttga
    27961 ttactatgaa gtagcaattt attatagata taatcattta ttgagataaa atttaattta
    28021 agggagtgtc attaattgtg tttgattatg gtaaaagaac ttatataatg ggaatattaa
    28081 atgtaactcc agatagtttt tctgatggag gagattttaa taacttggac atagcaatac
    28141 aacatgcaaa agatatggtt gaccaaggtg cagatataat agacctagga ggagagtcta
    28201 cacgcccagg tcatagctat gtagactcag acgaagagct aagaagagta atccctgtaa
    28261 taaaaaaact taaacaggaa ttggatatac caatatcaat agacacttat aaggcagatg
    28321 ttgcagaaga agcattaaaa ttaggtgtca ctatggtaaa tgatgtttgg ggtcttagaa
    28381 aagataaaaa tatggcttct gtaataggaa aatatgatgc agaagtgtgt ataatgcata
    28441 atcaagatgg gacaaactat gacaaagata taatggagtc aataaaagac ttttttaagg
    28501 taagtataga aatggcaatg agttgtggtg taaaaaaaga aaaaatagta cttgacccag
    28561 gtgtaggatt tggcaaggat tttgaacaaa atattgaagt attgagaaga ttaaatgaat
    28621 taaaagattt agggtatcct attttgcttg ggacatccag aaaatcagta ataggaaaag
    28681 tacttccagt tgagcctaaa aaaagattag aaggtacaat agctactact gttcttggta
    28741 taagagatgg cgtagatata gttagagtac atgatgttta tgaaaattta atggcagcta
    28801 gaatgactga tgcaatatat agaaagtagg tataatataa tggataaaat attacttagt
    28861 aatctaggat tctatggata tcatggtgtt cttaaagaag aaaattttct aggtcagaaa
    28921 ttctttgtcg acatggaact ttatattgat tcaagagaag caggtcttag tgacgatata
    28981 aataaatctg taagctatgc cgaggtttac aatgttgtta aagatattac tgaaaataaa
    29041 caatttaatc ttttggaggc tttagcggaa aatattgctg aagaagtctt gaataaattt
    29101 atacttataa atggagttat ggtcagagtt agaaagcctg aggctcctgt aaatggtatt
    29161 tatgactatt ttggtgttga gataaggaga acaagagatg aataaggctt atttgggaat
    29221 aggtactaac atgggtgata gatttgataa tttatcaaga gcatgtgaac ttttgaaaaa
    29281 tagtgattct atatataaag taaaagaatc aagcttatac gaaacaaaac catggggata
    29341 tacagaacaa gcagattttc taaatatgtg tgtagaaata gaaacagagt ttgaacctta
    29401 tgagttgtta gaatattgtc aagaaataga gagagaacta cacagagaga gaatagttca
    29461 ttggggacca agaactatag atgtagacgt actatttttt aatgatgtag tttccactga
    29521 tgaaagacta acaatacctc atccaaggat tcaagataga gctttcgttt tgataccact
    29581 tatggattta aatgaaaaac ttataataaa tgaaaaaaca ataaaagaac atttaaacct
    29641 cctatctgct gaagaaagag aagaagtaaa ggagcttgta ggttatgaaa gaaaacctat
    29701 ttaataataa ttcaaagata gagataaata caaataacaa aaagataact gtaaaaaaag
    29761 ataaacttat aatagctgga ccatgcgcaa tagagagtta tgagcaactt ttggagactg
    29821 ctaaatttgt aaaacgtcag ggagcaaata ttttaagagg tggagcatac aagccaagaa
    29881 catcgccaaa ttcatttcaa ggattaaaaa aagaaggtct tgaaatatta aaagcagtta
    29941 aagacgaagt tggtatggct gttattacag aacttatgga tgtaagagat atggacgaac
    30001 tgtatagtat aagtgatata attcaaattg ggtcaagaaa tatgcaaaac tttactcttt
    30061 taagtgaagt gggaaaacaa aataaacctg ttatgttaaa aagaggaata gcatccacaa
    30121 ttactgaatg gataggtgct tctgaatata tagcaataga aggaaatagt aatattataa
    30181 tgtgtgagag aggtataaga acgtataatg attatactag aaatacatta gatttagctg
    30241 cagtacccat tattcaaaag gaaacaggtc ttcctgttgt ggtagaccca agccatgcca
    30301 ctggagttag atatttggta aaacctatgt cactggcatc ttttgcatgt ggagcagatg
    30361 gaataatggt agaagtacat cctgacccag aaaatgcttt gtcagatggt gctcaatctc
    30421 tttgctttaa tgaatttgaa gatttaatga aatcaattaa taattattaa aagaggcaag
    30481 agcctctttt ttattcgtaa tcgttgctaa ggtaaattta taagttataa tttgatagtt
    30541 agattattta atataaataa agatttaata ttgatagaat taatataaaa aataaaaatt
    30601 ttttgattaa taagaaggat atcaataaaa aaagtcgaat tgtgtatatt gagtgattga
    30661 caaatttaga atatagtgta gaatagaaat gttaacacaa aaaattacac cagaaggtga
    30721 taatatgaat gatataaaag acattattga agaaataaaa gctagatgcg atatagcaag
    30781 tataatatct gaatatatga atataaaaca atctggagct aattacaaag gattatgtcc
    30841 atttcatggc gaaaagacac cttcttttta tataaatacg tcaaaacaga tttataagtg
    30901 ttttggttgt ggtgaaggtg gagacgtaat aaattttgtc atgaaaatgg aaaatttgga
    30961 ttttatggat gctgtaaaaa ttttagcaaa taaatgcggt atagaaatta acactaacat
    31021 gaatgaagaa actaggatta aaatagaaaa atctaagaaa tttcaagata ttcatacgga
    31081 agcagctaga ttttatcttt caaacttatt gggaagcaaa aatcttgggt atgaatattt
    31141 aagaataaga ggactagatg ataaaattat taagaaattt ggtttaggat tttctttgga
    31201 ttcatggaat tcacttatga atactctaat cagtaaagga tataaaaaac aagacttatt
    31261 ggagtgcggt ctaattgcaa aaaatagaga tggaactaat tgctatgata aatttagaaa
    31321 tagagttatg tttcctatat tcgattatag aggaaatata ataggatttg ggggaagagt
    31381 attagacgat tctttgccaa aatacttaaa ttctccagac acattaattt ttaataaaaa
    31441 acagaactta tacggattaa attttgctag aaaaaattta gaaagtaaaa ctatagtctt
    31501 agttgaagga tatatggatt taatttcact ttatcagtat ggtataaaaa atgtagtagc
    31561 aacccttgga acagccttga ctgaacaaca aggcatttta ataaagagat atgcggacac
    31621 tgctattata tcttatgatt ctgatgaagc aggaataaaa gcgactttaa gagctataga
    31681 tatacttact aaattaggta tcaatgtaaa agtgttagac ttaaaagatg ctaaagaccc
    31741 agatgaattt gtaagaaaat acggacttag tgattataaa aaagctatgg atgtttctac
    31801 tcactacata aaatataaaa tagatcatct taaaaaggaa tttaacattc aaaaagatga
    31861 agaacgagtg aagttcgcaa aagaagcttc taaaattata aaacaattga caagtcctgt
    31921 tgaaattgat ttttatacaa agtatttaag caatcaaata gatattaatg tcgagtctat
    31981 aaagagagaa gtttatggta aaaattacaa taagccatat aataacaaaa atcaaaagaa
    32041 aatagaagaa aaagtaattg aaaaagtcga agtcagacaa gacggaaaac aattagttga
    32101 agaaactcta ataaaaataa tgttagaaga caaaaaaatt agagaaatag cgttacttaa
    32161 agtagaagaa agtgattttt tattgaaaga aagtaaagaa attttaaatt acatgattaa
    32221 aaatcaagaa ttggacaaaa taactattga caaattgaaa agtttaaaca tatccgaaga
    32281 atacttaaaa gaattaaatt caatttcttt gaatagtata aacttagaga acacaaaaga
    32341 aatagaagga ataataacaa atatcagaaa aaactcatta gaagagcaaa ttaatagctt
    32401 gttgagagaa caacaagaat tagaaaataa taatgatatg aaagaggtag atggtagagt
    32461 tatggaaatt gctttaaaaa tagttgagat aaataaaatt ttaaagagct tgtagagaaa
    32521 ggaggtttga gtgtggaaaa taaatcgaat aaaaaagagt taaaaaaggt tactgctaag
    32581 acattaatag aaaaaggaaa aaagcaaggt tctttgacac ttgcagagat aatggaagct
    32641 ttttcggaga ctgaacttga taaggatcaa gtagaaaatc tttatgagac tttaggaaat
    32701 ttgggaatag aaataacaga aacaaaaaac tataaagctg atatagattt ttcggttgct
    32761 gatgacgatt taagtatagg ccacttagat gaagatgcag aggcaatttc acatgatgac
    32821 tcttctgcaa tagaaataga aactgtggat ttatctttac caaaagggat aagtatagat
    32881 gaccctgtaa gaatgtactt aaaagaaata ggaaaaattc ctctacttaa accacatgaa
    32941 gaagtagaat ttgctagaag gatgcacgaa ggtgatgaga tagcaaaaca aaggttagtt
    33001 gaagcaaact taagactagt tgtaagtata gcaaaaagat atgtaggaag aggtatgctt
    33061 ttcttggatt taatacaaga gggaaattta ggtcttataa aagcagtcga aaaatttgac
    33121 tatacaaaag gatataagtt tagtacttat gcaacatggt ggataagaca agctataaca
    33181 cgtgctattg cagaccaagc tagaactata agaataccag ttcatatggt agagactata
    33241 aataagctaa taagagtatc aagacaacta cttcaagaac ttggaagaga tccaaaacca
    33301 gaagaaattg caaaagaaat ggaaatgaca gaagataaag taagagaaat aatgaaaata
    33361 gctcaagacc ctgtgtcttt agaaacacca ataggagaag aagaagatag ccatttaggt
    33421 gactttattc cagatgatga tgctccagcc ccagcagagg cagcagcata ttctttatta
    33481 aaagaacaaa tagaagatgt acttggttca ttaaatgata gagaacaaaa agtattaaag
    33541 cttagatttg gtcttgagga tggtagagcc agaactcttg aggaagttgg aaaagagttt
    33601 gatgtaacta gagagagaat aagacagata gaagcaaaag cactaagaaa actaagacat
    33661 ccaagtagat caaaaaaact tagagattat ttagactaaa aagatacatc attgatgttt
    33721 ttgtgataaa tgccatgaga taagtgatat ctcatggcta ttttgtagta aagatttaat
    33781 tttatttttg tagttatttc agtctaatat ctataacttg gattaaatcc tataaataaa
    33841 gggaattgaa aggagaatta tatttgaaat taacagatag attattgaaa atagcatcat
    33901 tagtgtcaga tggtaaaaaa atagctgata taggaacgga ccacggatac ataccagtat
    33961 atttgttaaa agagggaaaa gtaccatttg cagtgctagc ggatgttaat aaaggacctc
    34021 ttgacaatgc acataaagaa gtcatacaga acaatctttt agataaagtt gatttacgat
    34081 taggttctgg tatagagata cttgaaatag gggaagtaga agaagtgata attgctggta
    34141 tgggtggaat cttgataagt gagcttctcg aggctaaaaa agaagtagca cataatgtag
    34201 aaaaattaat attacagcca atgcaagcac aagaagaact tagatattac cttttaaaca
    34261 acggatatga aattttagaa gaagttttag tgagagaaga ttttagaata tatgagataa
    34321 ttgttgccaa atatacaggt aaaaatacta taatagaaga tgagatatat tatgaagttg
    34381 gaataaagct tttagaaaac aaagattctc tttttaatga ttttatagaa aaaaagataa
    34441 agacatatag ttctatagtt aataaattag aagggaaaaa tggggaagct atagataaga
    34501 agagaaagga aagcgaagtt gcaataaaaa aacttgaaaa tttaataaaa taaataggag
    34561 gtatatatgc tattaaagag tctaactaga aaaattgaaa aaaagtatcc tctaaactta
    34621 gcagaggatt gggataatgt aggtcttata gttggagatt ttgatatgga tgtaaaaaag
    34681 gtattggtat ctttagaagc taatgaagat gttattgatg aagctatatc caaaaatata
    34741 gatttaattg tgacacatca tccttttata tttgggaaaa ttaataaaat taatagtgga
    34801 gatttaaaag gcagacttat ccaaaaactt attaaaaatg atatatcact ttattctatg
    34861 catacgaatt ttgatatagc atttgatggt ctaaatgact attttatgga aattatggaa
    34921 tttggtaatt caaaggtttt agatataact aaaagcgaaa atttgtataa attagcagta
    34981 tacgttccac ataactattc agatgaactt agaaaggttt taagtaattc aggagcaggt
    35041 catataggga attatagtga ttgtacgttt tcaacagaag gtgaaggaca atttaagcca
    35101 ttagaaggct ctaatccgtt tcttggaagt gtaaatgata tagaaactgt aaatgaagtt
    35161 aaaattgaaa ctgtagtacc acagaagctt ttaggtgggg taataagttc tatgttagat
    35221 gcacatcctt atgaagaagt tgcatatgat ttgtacaagt tagaaaataa aggtgagata
    35281 tttggactag gtagaataag taaatttgat aaaagtatga cattagaaag tctttcaagg
    35341 aaaataaaag agaaattaaa tatgaaacac ataagagtag ttggaaacct tagtacagac
    35401 ataacgaaag tagccgttgt aacaggagct ggttcagaat ttgtaaaaaa agctaagaga
    35461 caaggtgcag aagtactaat tacgggtgat gtaaagtatc atgaggcaca agatgcactt
    35521 gatattggaa tgtgtatagt ggattgtggt cattttgata cagaagatat attcaaaaat
    35581 gttatgaaga gatttttaga tgaatttagt gaaattgaag taataaagag taatgtatat
    35641 ttgaatccat ttagtactat ttagcagtgt ttgataataa aaatactata taaaaaataa
    35701 atttgagttt aattaggggg ataatgatga aagtagcagt aatttttcat agtgtttgtg
    35761 ggagtacata tttattagct agagaatata aagaagcgct tgaagaaatg aatatcgagg
    35821 tagatatatt tagagtaagt gacgaagttg caaaaacact tcctcaatat tatttgataa
    35881 attccaaaga atacaaagac gaatttgaaa gtataaatgt aattaaatct ggaaaagaaa
    35941 tattagatta tgatgctata tttatgggtt caccaacata ttatggaaat gtatctggac
    36001 caatgaagat gtttatggat agtttttctg atatttgggt aggtgctcct ttgagtggta
    36061 aaatatttgg ttgttttgct acagcaggtt cacaacatgg aggaggagaa ttagctttgc
    36121 aagctatgaa tatatttgcc caacatatgg gaatgactct tttgtcagtt ccatgttcag
    36181 taagaggagg ttatccagcg tatggaatac ttcatatagc aggagataat tctgatataa
    36241 gaccaaatga tgatgccaag ataggtataa gagattacct aaaaagactt aacatataag
    36301 aatatcatat aaaaatacct aaaagcgact gtatatactt ttaggtattt ttacttaatt
    36361 ataaacgtga agctttacca gaaatatggt caatttctat tttaacaaca gttgttaagt
    36421 tcatatcctt ctctatgtac ttcatgcctt cttcaaagaa tcctttagaa tattttttta
    36481 ttatttctac aagagcattt ttcttttctt cattttctac agtgcaagct tttccaaaaa
    36541 ctatagcact agaataagta gtactaaatt tatctggaat aacactttca ttagcaacta
    36601 ctaaaaaaga aactttgttg tttttcgata tattatctaa cttatgccca tttcttgcac
    36661 aatgaaagta aatagaatca ttaaagtata cataatttac agcaactcca taaggataac
    36721 cattttcaga tatagtggaa aaagtaccaa attcaccatt ttttaatact tcaacagtat
    36781 cttcttttgt catttctctt tttttcaatc tcatttcttt aaacataata tcatcctttc
    36841 taattttgat gcttaaatta aattttagaa catttataaa gattatgcta tgttttttac
    36901 aaattttaag aaaaaaatat agtttaagat aaaaggtagt gaaagagaag ctattttgag
    36961 gtttatttat gatattattg ttagagtagt acaaacaata ctaataaatt taatagctgg
    37021 tttcttgtta tttaaggtag taagcaggtc tataagaagg ggaagttatc gagggtagaa
    37081 gttgttgttt acaacgatat tataaagaaa atattattat ataaaataat aaatagtaaa
    37141 gaggtatcaa gttgaatata atacttaata cctcttcaaa attaagttat aaaagtgttg
    37201 tgggaattgt cctatacatg gtaaggagat aataaagaat gtatctttaa agtctcaact
    37261 aaacagcaaa atttatccaa caaacataca ataaatgctt ctttgtatct aggtggctta
    37321 acagtaagtg gccacatgtg ttttaaaatt atgtccgttt ctttttcatt tatttgaaaa
    37381 aataaagttg cattttttaa tgcttctcta gggtgagtaa atccatgtaa tccttttcta
    37441 tcacctttgt aatgccaatc ataaagaaaa aagtcgtgta gtaaagctcc tctaataaca
    37501 ctttgaatat caacacttaa gtgcaatttt ttacataata gataactata atatgctact
    37561 gacaaactat gttcaagaca tgaaacattg ccatgatgag gaaatgttct cataagttga
    37621 actttttcag aagaaagtat ctcagaagca cattcattaa aatatttgat ttcagctttt
    37681 gacaacatta tgaaaatctc cattcttagt aatatataaa tactatactt caacgataat
    37741 atgaaatatt tcttatccta caaaatataa tacccaataa tacaagtaag atacctttta
    37801 tggtatttta aagtaagaga gattttatta ggctaaacaa tgaaaaaatt aaaagtaaag
    37861 ctatagcaat atatagtatt tttccaagag gattgccaaa atttatagtc catccaatac
    37921 caaacctttt attaaccatt aatgatgggt catttggatt attatacatg atgcctgcta
    37981 tccaatactt ctcatcttct tctggagaat aagatgagtt gaattttaaa tttggggaat
    38041 taatatatgt tgctattaaa taaattataa ctaaaagcgt tgtaatattc atagcagttg
    38101 atagttttgt ttttattgat aaaagagtag taaaaaactg tgttgtcatt ataatcatca
    38161 atattaaaaa caaataacca attttattta aatatttaat gttttcaact ctactttctt
    38221 caattctatt tgtatctatc ttaatccttg actttatgga tccaatggaa gttatatata
    38281 ataaaaccat taaacaaaag tcagtaccga ttaatttaaa tacattaaga aatgatttat
    38341 ctataaaaac atcaggagac ccatttatgt tccaatgagt ggcaattgag tctggcaatt
    38401 gattatagtt aaatgctata aaaatactca ttcccaaagt aaatagtatc ggtattaaat
    38461 agattatttt aaattttttt attattttat ttttcttatt tataaaatcc atatctacta
    38521 ttaattttga atctatatct atatgaccta atttagcata aatttctgat ttaagcttct
    38581 tagctttttt atgtgtatga atatatagta tactttcata taataaaaaa cctataatac
    38641 caataaaata ggaaagttcc aatttattaa atgtaaaaac cagaataaaa actaatataa
    38701 agtctataat aaaacctaaa gataatagtt ttttaaattt tttatctaac tttataaagt
    38761 aattataatt tttatgctct tggtctatag aaactccata gatatgtttt ttacctacaa
    38821 aatagggcat aaaataaaat gtagtataca tcaaaattag aactggaaat attgtaaata
    38881 gacattcaat cttactcata atatttcacc tcatacttgt caaataaact tgaacttagt
    38941 gacaaaaatt cttctttact aatacctgat aaataagatt ttgccaccaa taattccaag
    39001 tcaagtttat ttttttgacg agttgtatta tctattttca ttggtagttg agctacaata
    39061 gcaccttttc ttctatcaat atttaagaat ccctcttctt ttaattcatt gtaggatttg
    39121 ttcactgtat gtaaatttac accaatattt tcagccatac ttcttacaga tggaagtgat
    39181 tcatttattt tcaattctcc tgaagctata gccttagtta tttcttcttt aatttgtaca
    39241 taaatagatt tatcactatt aaaatctaaa tttaaaacca taaaaaacct ccattatgct
    39301 ttataaatga aaacgattgt tatatacata gtataacata aaaaataata acaaacacaa
    39361 aatataagtt tatattttgt aaaaattgta atttaaaaaa taacataatt tttattaaaa
    39421 taatacagat actatttata gatagaaata aaaattagaa aggagaaaaa atatgttaac
    39481 atatttaatg aaaagacaaa tgcataaaaa agatgatgga atgagtaaac caatgatgat
    39541 tacaggagct gtaattacag gtgtaggagc atatgctgta tataaaatgg taaaaagcca
    39601 taaatctaat tctcaaatgg tcaatacaaa ttattatgga aatagcgatt atgatgatta
    39661 taaatatgac gaatttgatt atgactgtga ttgtagagac acaaaacaag attatgaata
    39721 tgaagaatta aaaaaaaaag taaaagaatt taattcaaga agaataaatt gtgaaaattg
    39781 tccacatgtt tctcaagaag aaatgatgca atatgtaaat agtgttaaag atgtaaagga
    39841 agagattgat ttacatgaag atgctaaaaa agataatatt tacaaagaag atgaatacaa
    39901 aaaatatgaa gaagaataag taatattttg tataactgaa caaatagtaa tgtcaaatta
    39961 tttaatatct agttttaata tgtaagttgt aacttaactt gaaagaatca gtatcattct
    40021 ttagatagtt aagtaacaac ttacatttta tttttataaa ttttaatagt tctttgtgac
    40081 tattttgtaa ttttagaaat attttagtat ccagggtaat tgtgtataat aaaacttgta
    40141 tagatagata gtatgaaatt aagcagatag tttaaaatta aaatgaaaag aaaggaaata
    40201 gttatgttaa agttgttgat agttgaagat gatagtacta tatcttttgg aataaagtat
    40261 gctttagaac aagaaggatt tagtatagat atatcaaagg atttatctag tggaaaagaa
    40321 atgatttctt ctaatgaata tagcatgata ctgttggatg ttacattgcc agatggaact
    40381 ggttatgagc tttgtcaata tataagagga ttctctcagg ttcctataat attcttgact
    40441 gcttgtgatg aagaagtaaa tattgtaatg ggtctcgata taggtggaga tgattatata
    40501 acaaaacctt ttagaattag agaattaata tccagaataa aagctgtttt aagaagaaaa
    40561 ggtaatactt cagaagaaaa taagaaaata cttaaatttg gagatttaag tatatataca
    40621 ttagaagcta gagtatataa aaatgataaa gaaatatttc tgacatcagt tgaatataaa
    40681 cttcttttaa ttcttataca gaataaaaat actgttttaa gtagaactca aatattggaa
    40741 aaactttggg atgtaactta tgattttgta aatgacaata ctctcactgt ttatataaaa
    40801 aggcttagag aaaaaatagg tgatgattta tgtgaaccaa tctacataga aactgtaagg
    40861 ggaattggtt ataaatggat aggaagtgaa aatagtgttt ctatataatc cagaagtgaa
    40921 gaagttttta agtaagtatg ttactttgat gtttattgtt atcataatat ctattggatt
    40981 tagcataatc aacgtgtcct taactaaaga gatggttgta agaaataatc aggctataat
    41041 aggaacttta tcaagcaagt atccaaactt agaaagtcag atagtagaca taataactca
    41101 agggaagtct atggagaaca tagattatgg tcagaaaata ttaattaagt ataattatga
    41161 taaatctatt aggataaact ctgagcctat tatttctaaa ttagttttag agactatcaa
    41221 aataaatata atattagtat gtataatatt tattttgata tttgtgttgg ttgttagata
    41281 ttttaagtca atttacaatg atttatcaga tatgactaag tatgtttact atagttcaga
    41341 gggtaagcca tttgatatga aaaacaagaa tcaagaaggg cagataggtc ttctaaaaac
    41401 tgaactctta aaaatgacga caattcttaa tgaaaaggtt gaacttttaa aaagagaaaa
    41461 gatattttta aacaatacaa tttccgatat atcacatcaa ttaaaaactc ctatgacttc
    41521 tttaataatg ttgaatgatt tattatataa tgatgtacca tacgaagtaa aaatcgattt
    41581 tttaaataag ataaaaaatc aattaaatag aatggattgg ttgattaaaa gcatgcttaa
    41641 attatcaaag gtagaagcaa aagtgataaa ttttaaaaaa gataaggtaa aatttagtga
    41701 actcatacat agagcaatgc tatctatgaa aatacctatg gagattaaaa atcaaaaact
    41761 gattatagag ggaaatgata atgtatctta tattggtgat attgattggt ccgtggaagc
    41821 attagtgaat ataatcaaaa attgtgtaga acatactcct gaatttggaa atataattat
    41881 aacctataaa gaaaatccat tattttctga actaataata aaagatgagg gtgaaggtat
    41941 agataaaaaa gatataccac atgtatttaa acgattctat agaggaagaa gtagttcaaa
    42001 agaagatagt gtaggaatag gtcttgcaat gtcaaaatct ataatagaaa gccaaaatgg
    42061 cgatatatat gtaaacagcg aaaaaggtaa gggtacagaa tttcatataa tatttcataa
    42121 aatgtatgat agtgactaat ctgtaatatt taattcactt tatagtaata gccaaaacct
    42181 aatatatgaa gtatagaata taaatattgg aggtagttat ggaaatttta agagtagaaa
    42241 atataactaa aagttatggt aaaaatgaaa caaaagttga tgctataaaa aatgtaagtt
    42301 tatcagttga aaaaggaacg tttatagcaa taacaggtcc aagtggaagt ggtaaaagta
    42361 ccttattaca tttattagga ggagttgaca gaccaactag tggtaaggtt tatataaatg
    42421 atgttgatat ctataattta aaaactaagg atttggcgat atttagaaga agaaatatag
    42481 gtcttatata tcaattttac aacttaattc cagtcttaaa tgtgaaggaa aatatattgc
    42541 ttccagcaga gcttgacaat agagatattg atagagaata ttttgatgat ttaatgaaaa
    42601 cattaggtct aattgataga gaaaagcacc ttccaaacca attatcagga ggtcaacaac
    42661 agaggacctc tataggacgt gcattaatta atagaccttc tataatatta gcagatgagc
    42721 caacaggtaa tttagatagt aaaaattcaa aggaaatatt agaattgtta aaattgtcag
    42781 taaaaaaata caaccagaca ctaataatga taactcatga caaaaatatg gcacttcaag
    42841 ctgatagaat tataagcata gaagatggaa tcataaaaag tgatgaggtg atgtagatga
    42901 atttatatac atcactaact attagatact taaaacaaaa taaaagaaga accattgtaa
    42961 ccataatcgg tataatactt tcaacagctt taatttgtgg tataggaaat atatttgaaa
    43021 gttttatgga ttatcaaatg agagaaacta taaagaatga tggaagtttc catgttacat
    43081 tttatgatgt aaatagaaga aatgtggaat atgtaacgaa atcagctgaa atagaaaaac
    43141 atgcatttac caaacaactt ggatattcta aattagaaaa tagtgaaaat gctatattaa
    43201 gtataaagca atatgataaa aatgcaataa acggatataa agtttctata aaagaaggta
    43261 gatttccagc taaggaagga gaaattgttt taagtgaaag tattattaat cttatagatg
    43321 ataagttaaa aataggagat aagataactt taaaagtagg ggatatgttt gactcaagca
    43381 agaaaaagat tgatagtatg acatttcatg aaggtgatta tatagcaaat gaaaaagata
    43441 gaacttttaa agttgttggt ataattgaaa aacctgggtt tgaacgttat aatggaattg
    43501 caacagctat agcctacttt aatcctatga ataattacaa tgatacaatt aatgtatcag
    43561 tagcagtaag aaatcctaaa gatgtgtata agataagtaa taaaataagt aaaaatatat
    43621 attcaaataa agataaagaa gcagtttctg atatgataaa atacaatgaa catttattga
    43681 ggttacaagg tgctagtaag tattcaaata taaatagctc tataaagtct ataattatag
    43741 cagtaactat tttagtaatc atatgtacaa ttgctacagt gtacaattcc tttagtattt
    43801 ctataaatga acgaaaaaaa cagtttggga tattaaattc tataggtgct acatctagtc
    43861 aaattaaaag attagttttt atagaaggta ttataattag tttaattggt ataccaatag
    43921 gactaatatc tggaacagta gctatagact tattgtttaa aattataaat aagtatttta
    43981 ctgaatcggt tgttactaag atgagtttgc agatagtgta taatccaata ataattattg
    44041 ttagtataat aatagttttg tttacaatct ttatatcaat tctactacct gcaatatcgg
    44101 cttctaatat ttctcccctt aatgtgatta aaaatactgg agagtataaa gtgggcaaaa
    44161 tcaaaagctc aagacttatt aaaatgattt ttaaaactga aggagtactt gcatataaaa
    44221 atattaggag aaatagaaaa aaatttataa taactctttt ttctttaatg gtaagtataa
    44281 taatatttat atcttttagt ggctttacat tacttttact aagaggtgaa gaaattagaa
    44341 attctcaaag aaaatatgat ttgtatttaa ctgcaaagaa cacagctagg tctgtagagg
    44401 atactataag tgaactagaa gatattgatg ggataaaaaa ctttgaattg gcaacaggac
    44461 aatatgtgtc tataagagta agtgaaaata aaataaataa atccaaggaa gatttaatca
    44521 ggaaatatta tcaaaaatat aagattggag atagttatga atatgatttc agtaataatg
    44581 aattaatttt tccaggagat tttgcagtaa aaaacaaaat aaataactta gtacagggct
    44641 cttttaataa agagagagca attgaagaaa atggtgttat attagttaga aaaagtgcat
    44701 ttgaagaaaa tgggaagaaa ggtgtagtag aacttaccaa ctataaagta ggagatacag
    44761 taaattgtga atatcttgat gaaaatgcct taagtaaaaa agtaaaggtc aagattcttg
    44821 ctataactga tgaagaacgt ctaggtcttg gttatcaaaa tatggggtta caatttataa
    44881 cttatgatga agttgctaaa aatttaaatt taaaattaag taaaagtctt atatttattg
    44941 attcaggagg aagcattaag actaagaagg aaatagaagc tttggcaaat aaaaataatt
    45001 ttaactttta tgatgaaagt gcaagcaatg aaagtgaaaa acaagattta aaggttatta
    45061 aaatatttgt atatggtttt atagtagtga tatctttagt gagtgttaca aatattctaa
    45121 atacagtaag tacaagtata aatctaagaa aaagagaact tgcagtaata caatctatag
    45181 gggttactcc taaaggattt aggaaaatga tatatttaga aagttttata tatggtatac
    45241 tgtcattgtt gtttggaata ccaattagta ttggtataat tctaattatg aataaactta
    45301 tttcaggtgt aattgaattt tctccgatta taccgtggac agccatagtt atctgtatag
    45361 taagtgtttt tataataact tttatagcag ggtatatacc aatgagtaaa ttaaataaag
    45421 aaaatataat agataatata agaagagaaa gtatatagat ataaaaataa ttcttaacta
    45481 tataaaatat tattaaaatc atttaagtgg tataaaatat tattatatca cttaaatgat
    45541 tttttattta tataagaaat ctaataataa attactagag gtatttaact atttagaaag
    45601 aattggtatt atataaagta agagaatata taataagaaa gaatatttga ttttattgga
    45661 aaacaaaaga aaaagaaggt gattactatt tctcatataa ctgcgaagaa tatgtataaa
    45721 agtttagagg aaagaataaa caagtttcca caaggtgctc caccatctga tacattatac
    45781 aaaatactaa atgtactata tacagaacaa gaggcgaagt tagttgctca gcttccaata
    45841 aagcctttca gagtaaaaac agcagctaaa atttggagtg taagtgaaag tgaagcatat
    45901 agagtattgg ataaacttgc tagtaaggct ctgatactag atatagaaga taataaaggg
    45961 aagaaatata taatgcctcc tccaatggct ggtttttttg agtttgccat gatgagaacg
    46021 agacacgata tagaccaaaa acttttggca gagctttact atcaatatat gaatgtagaa
    46081 gaagatttta tcaaagattt attttattca acagaaacga agcttggaag agtatacgtt
    46141 caagaggaag ttttaactaa tgataatgag gttagtattt tagactatga aagggctact
    46201 catataattg atgaatcaac tcatataggt ataagtatgt gttactgtag acatagaatg
    46261 caacatgttg gaaaagcttg tgatgctccc atggatatat gtatgacgtt tgacaatgta
    46321 gcaaattctt taataaataa taaatttgca agacgtgtgg ataaaataga atgtaaagaa
    46381 ctacttcatc aagcatatga acataactta gttcaatgtg gagaaaatgt taggaaaggg
    46441 gttacgttta tatgtaattg ctgtggatgt tgctgtgaag caatggtagc agctaaaaga
    46501 tttggaaatc ttcatccagt acagacaaca agttttatac caaatataaa ccatgaaaat
    46561 tgtgtaaagt gtggtaaatg tataactgca tgtccaatag atgcgataag caaggttaag
    46621 gaagatggta aagaatatat aaaaattgat gaggatagat gtttaggttg tggagtttgt
    46681 gttagaaatt gccataaaaa tagcattatg ttattaaaga gagatgaaaa aataataact
    46741 ccagcaaatt cagtacatag agcagttctt atggcaattg aaaaagggca gttacaaaat
    46801 ttaatttttg ataataatgc tctggctagt catagagcta tgggagctat actgtcagct
    46861 atattaaagc ttgaaccagc taaaaagatt ttagccagta aacaattaaa gtcagtatat
    46921 ttagataaat tgttaagtat gaatgataaa tagaaactaa cttaaaaaag gcaaagtttt
    46981 agacttatct atatcttaat aaacttatca tttaatattt aaatttaatt tttacacata
    47041 taaaacttat aacttaaata aaaaaaggct atattcaatt aatattactt gaatacagcc
    47101 ttttttagtt atgttaagct atttctttta tttgcttaaa tgagttttca tttccatttc
    47161 caccaatttg tgttacagat ttaaatctca tagttttaaa tagttctttt tgattataat
    47221 ctagtgagtt tcctactagc attacaggag atttagtttt tccagccaaa actccaacag
    47281 ataatccgtc tattaaatca tcttgtttat tcattccatt ttttgcaaca tataaattgt
    47341 ctatcttaga atccttgtaa aattctttta ttacttttgc attagtttca tttctagtgc
    47401 tacctgatat tctctgtgga ttttgaagtt tactttctat atttttagaa actgtatatt
    47461 ctccaccgat tacataagat ttatcaatct ttttgttttt aaataagtta tttatttcag
    47521 ttatattact attttcatta gtaagtatta taggcatatc attttgagct gatacagctc
    47581 caatgcttac tgcatcggct aatccttttt cgccattaac tactgaaatc tttgatatat
    47641 tgctaatatt gttcaattct ttagcaatgt tcatagaagt ttgatatcta tcacttcctg
    47701 aaattctaga tacagagata ttgtttgatt ttatagtatc taaagatttt tcatttatag
    47761 aagcttctcc accaactaca tatacatttt taacttttaa tctctttaat tcagccaaag
    47821 ttttttcatt tatttgagag cttccagtca ataatatagg agcgtttttc ttgtatgcaa
    47881 atggtgttgc ggccaaagca tctgcaattg ctgaatcgtt tattaaaact gcattttcag
    47941 cattttccca acctgcttga cttatcttaa tagaagtttc atatctatca gttcctgtaa
    48001 gtgcacttgc atttacttca tcttgatatg taacctcaaa tctagcattt ggacttccta
    48061 agaaaacaat tacgcctcct tttggcaatc taactctatt gttctttgta actaaagaat
    48121 agtttacagg aacaccttta tcatcataaa cagcaaaaga tgaattttga ggaagattaa
    48181 cttctatttt tttatttgcg agatcgtcac caatcttata ccattttgca taaccattgg
    48241 attctagttc acaagtaaaa gatttttctg ctggcaaatt agttatagaa tcttcactaa
    48301 catacgttag tgttccaaaa gataaatact ctgtaccatt ttctttatga agtttaatgt
    48361 cggataaatc acgacctatt acaccaggta tttctataaa agcatttgag ttattctcat
    48421 ccattatttt agtgttaact atataacctg gagtttcatc agaaagagca agagttgcct
    48481 tcacagaacc aaacatatag ctttgagaag tatatttttc atctactaga tagtaatctt
    48541 taccatttct ttttttccat gcttctttta cactatttga tatattgttg tcatctatct
    48601 tttgagctac atagtataaa cttgctgtct gacctaatcc agggacatca tcataagagc
    48661 tcatatttaa atatgtgata ttgttttttt cttttacaaa ttttagacag ctgttacctt
    48721 tttcagatac aaatctatct tgacctatgt atacatattt atcttctggt ccattttcta
    48781 tataaggaga agatactgtt aaagtaccat tatcattaac atctactttt atcatatttg
    48841 aagaagcata aagaccacta ttttctttta gagagctagg cattttaact tgttgtggct
    48901 tactaaatgt tttatcaggt tttatttctt taattttacc tttttctttt aatgcagata
    48961 gtaagatttc ttgtccaatt atttcgttaa gttgactact tccaccagaa gaaagaactg
    49021 caactgccat attttcatct ggaagaacta taagattact gtggaataat aaagagtctc
    49081 caccttttgt aagagctttt aaattatact gattaaatgg ataagtgttg acacaatccc
    49141 accctaaacc atatccaagt atagagtctt ctccttcagg ccatagacca tttaaatatt
    49201 ctttgttttc catagctttt actgaagcag gagaaagaat accatttgaa tttttcataa
    49261 atgtttgtgc aaatgtacat agattttctg cacttgaata taaaccacct gcacctatag
    49321 cattaaggtt atcttgcgga actgcatctt cccaataagg cacgtatgct tttgctagtt
    49381 ttgaactatc aaaactattt tctggagttt ttgtattttg taagttaagt ggattattta
    49441 tgtatttatc aagaaaatta gtaaatgaca taccagatac tctttctact aaaatttcag
    49501 ctaaagtaaa tccatcatta caataaacag aaaaagctcc tggtttagct tttaatcttt
    49561 gtttttgtaa ttcttttaag aaattatcat gaccataaga atcattgtct gctaataaaa
    49621 tagtgttttt aaaactactt cccatcaacc ctgatgaatg atttaataac attcttggag
    49681 tgatttcctt gtatctatca tcagccattt taaattcagg tatatacttt acaacaggag
    49741 tatctaaatt taattttcca tcatcaacca acttcataac agctgttgta gtaaacattt
    49801 tactgataga tgcgatacta tacatattat ccttatttaa gtttttgtta tcctgtttgc
    49861 tgtaaactcc accattacca gaaatctcaa ttttaccatt atcaatcaaa gcatattgag
    49921 cacttacagc accatacttt gatagcaaag tttggatttt ctctttagca agagctttag
    49981 tttttgtgta agattccgcg tttttactat aatcatgtaa ttctatatca gaactttgat
    50041 atttttcgga attatctttt gtattatcag caaaagtaat atttggagca gcagtaaata
    50101 acatactcaa tactaatgta ctagataata atttagagag cctttgctta aacatattac
    50161 attacctctt tcccttttta ataatttata attagcataa cattcttaat tattttaatc
    50221 aacagattat tatcaataaa aaaagctttt ataagtatag ctttttatat aacagagaaa
    50281 aatataaaaa taatatttgt tgttgtataa agaataaagg gtataggtaa tatataaata
    50341 aaatggagga attttagaca tatgtataag aatataaata agaagcaact taaagaaatg
    50401 atgaaaaatg aaaaggatgt tttattatta gatgtaagaa ctaaagatga atttaaggaa
    50461 tataaaatag aaaatgctat aaatatatct ttacaagagt taatcaataa tatagatgaa
    50521 atttacgatt ataaagataa gaaagtagta gtttattgca gaagtggtca tagaagtgtt
    50581 actgcatgta atttgttagc tgaagaaggt tttgagcatt tatataattt aaattcagga
    50641 ataattgatt atatgagtta aataatgtaa gttatacatt aaatactatg tatagctttg
    50701 cttattaagt aggaatatat ctaaatattt ataagataaa tttaggaaag aacgaatttt
    50761 ttcgtttttt tctatgacag tttttgtagt aaatattagt ttttaagttg gaaaaaggta
    50821 gtagcttttt agctactacc ttttaatatg aacttagaat attattaaaa aaatatattt
    50881 aaagacagaa ttaatttttt aatgaccagt taaattacta tcaccaaatt tatcattaaa
    50941 tggttttata actctattta taggtccttt tagaagtaca tttaaacaaa tagacagaat
    51001 cataaatagt aatagaacta gtatatcttt agttagattt ggagcatata taccaccaac
    51061 tgcttccctt gtagcaccta ttgcataggt aaatggcatt atagggttta ttctttggaa
    51121 gaatgtaggt gtaacttgta tagggaatgt accaccagaa gaaccaatct gaacgactaa
    51181 tagtactatt gcaattgctt ttcctacatt tccaaataca gaaactaaag aatacactat
    51241 aaagttaaat accatactag taaacataag cagaactatc aataaaattg gatgttttgc
    51301 tgttactcct aacaataata tatctccagt tcctattata aaaccttgga caagagctat
    51361 acttaaaaaa gtaaggcctc ttccaaagta aatttcataa ggtttatagt ttccatgtac
    51421 agatgtagac aatagtgctg aaataagtaa tataccaacc catgtagaaa gaacactata
    51481 aaatggtgtc attgcagaac cataattttc aattggatat aatttctctg ttttcacctc
    51541 aacaggttct tttaagaaat tagactttgt caatatatca ctttcaagca agctgactaa
    51601 ttttttcata tcttcaccat tactaatttt gcttaaagta tcaatcaaat catccaacat
    51661 tccttttgct aaaggaagtc tttctcttat caatgatata ctttcctgtg catttcctga
    51721 aagttttaga gaagtagtaa gtatttcttc aacctttgga agttttgctt ctgccttatc
    51781 taaaacagta attatatcat ttgctacttt tatactgttt gcaaatatac tatttatagg
    51841 ttttgatatt tttgagtcaa aattatttaa aatatttaaa ttaattcttc caataccatt
    51901 tgatagactt aaaacattat tcaaagcact tatagatggt tgttgtccag atataacttt
    51961 gtttttaata tcatttaatg ttgatataga tgaatctatt ttattgctag agtcttcaag
    52021 attatctatt acatcatcca atctattatt tgatgtaagt tggtttaatt tagttaaaaa
    52081 gtccaccaaa gtatcgttta aagattgcaa attagaaagt ttttctgata aattatctat
    52141 caacttaggt acatcttcac tgccactatt tactgcatca attaaattta atgtaagaga
    52201 agaagcagaa gatgaaaggt caaccattaa atttaagtca gacttaataa gaggagaaag
    52261 ttcgtctaaa ttatcatttg tatcctctaa gaattttttt aagtcacttg ataatagttt
    52321 tgtatcattc agagtctttt taattgtagg taaatcagat ttaatatctt taactgtatc
    52381 agataatttt gaagtagcat cagaagctaa atcaacagtc ttatttatat ggtcaaatct
    52441 cttttgaagg tcaataaggg aattttcaac ttttgtcaat tttggaagac tgtgttcaat
    52501 tcctgcacca actccgtttg atataccaag aacgatttca ctaactgttt ttactatagt
    52561 ctgattaacc tgtagttgta ttgtagaagc acctttatct gtaatctttg gtgcaactgc
    52621 atttattttt tcattaacag tatagataat ttcgcctttt ttaacatcat ctgtaattaa
    52681 agatgtcaaa tcttttgtaa aattttttgg aatcttaagt gatgcataat aagtaccttt
    52741 tttaacacct tcaagagcag ttttttcatc tacaaactgc caacctaaac tcttgttatc
    52801 tttgagttgt tcaattaatt ggtctcctac attaatcttt tgtcctgcta tttcatttcc
    52861 tttatcttca tttgttactg ctacaagcat attagcagtg cttccataag ggtcccaaga
    52921 tgcctttata ttgaaccaag catacagaga tggtaaaaaa gcaagaccta aaactattac
    52981 aagtacggca taattgccac gtatatcttt taggtcactt ttaaaaattt ctaatatatt
    53041 ttttaaaccc tttgaaggtc ttaaattctt gatattattc cagtttaaaa tttcttttaa
    53101 actattagtt tttttcaaat gtctcacctc ctaaaatatt ttgataaaga aaaaagttag
    53161 atttataatc aattttaaat tatattactt tttacacaaa tagtaaagtt
//
LOCUS       DAMJYC010000020        52714 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJYC010000020 DAMJYC010000000
VERSION     DAMJYC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.021"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            749..1411
                     /locus_tag="PC472_001248"
     CDS             749..1411
                     /locus_tag="PC472_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726583.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1420..3927
                     /locus_tag="PC472_001249"
     CDS             1420..3927
                     /locus_tag="PC472_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726584.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSVIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4258..4602
                     /locus_tag="PC472_001250"
     CDS             4258..4602
                     /locus_tag="PC472_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726585.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKQLTVTGNEYAIINY
                     YMSNFSQYIIFAILLASMRFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            4840..5526
                     /locus_tag="PC472_001251"
     CDS             4840..5526
                     /locus_tag="PC472_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726586.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            5537..6778
                     /locus_tag="PC472_001252"
     CDS             5537..6778
                     /locus_tag="PC472_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2726587.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            6849..7532
                     /locus_tag="PC472_001253"
     CDS             6849..7532
                     /locus_tag="PC472_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726588.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            7529..9862
                     /locus_tag="PC472_001254"
     CDS             7529..9862
                     /locus_tag="PC472_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2726589.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(9970..10532)
                     /locus_tag="PC472_001255"
                     /pseudo
     CDS             complement(9970..10532)
                     /locus_tag="PC472_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10646..11995)
                     /locus_tag="PC472_001256"
     CDS             complement(10646..11995)
                     /locus_tag="PC472_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2726590.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(12032..13252)
                     /locus_tag="PC472_001257"
     CDS             complement(12032..13252)
                     /locus_tag="PC472_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726591.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(13621..15633)
                     /locus_tag="PC472_001258"
     CDS             complement(13621..15633)
                     /locus_tag="PC472_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726592.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNRKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            16104..16754
                     /locus_tag="PC472_001259"
     CDS             16104..16754
                     /locus_tag="PC472_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2726593.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(16894..18270)
                     /locus_tag="PC472_001260"
     CDS             complement(16894..18270)
                     /locus_tag="PC472_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2726594.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(18286..20199)
                     /locus_tag="PC472_001261"
     CDS             complement(18286..20199)
                     /locus_tag="PC472_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2726595.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            20335..20967
                     /locus_tag="PC472_001262"
     CDS             20335..20967
                     /locus_tag="PC472_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2726596.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            21106..21645
                     /locus_tag="PC472_001263"
     CDS             21106..21645
                     /locus_tag="PC472_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2726597.1"
                     /translation="MVFVEELWKKAIKFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(21821..21991)
                     /locus_tag="PC472_001264"
     CDS             complement(21821..21991)
                     /locus_tag="PC472_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2726598.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            22276..23343
                     /locus_tag="PC472_001265"
     CDS             22276..23343
                     /locus_tag="PC472_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2726599.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            23513..25414
                     /gene="cooS"
                     /locus_tag="PC472_001266"
     CDS             23513..25414
                     /gene="cooS"
                     /locus_tag="PC472_001266"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2726600.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            25407..25880
                     /locus_tag="PC472_001267"
     CDS             25407..25880
                     /locus_tag="PC472_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2726601.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            25855..27081
                     /locus_tag="PC472_001268"
     CDS             25855..27081
                     /locus_tag="PC472_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2726602.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(27220..28407)
                     /locus_tag="PC472_001269"
     CDS             complement(27220..28407)
                     /locus_tag="PC472_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2726603.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            28680..29504
                     /locus_tag="PC472_001270"
     CDS             28680..29504
                     /locus_tag="PC472_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726604.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNMNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            29633..30898
                     /gene="gluD"
                     /locus_tag="PC472_001271"
     CDS             29633..30898
                     /gene="gluD"
                     /locus_tag="PC472_001271"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2726605.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            31111..31701
                     /locus_tag="PC472_001272"
     CDS             31111..31701
                     /locus_tag="PC472_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2726606.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            32031..33164
                     /locus_tag="PC472_001273"
     CDS             32031..33164
                     /locus_tag="PC472_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2726607.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            34080..35129
                     /locus_tag="PC472_001274"
     CDS             34080..35129
                     /locus_tag="PC472_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2726608.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSVNQNGDSTNNNNQQNNANNIQQDNTNDNQQNNNS
                     NVPTANSNAVQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSK
                     AQSKYGQTVSKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSIN
                     SSYYTARFVTAKRIL"
     gene            35425..36345
                     /gene="pyrB"
                     /locus_tag="PC472_001275"
     CDS             35425..36345
                     /gene="pyrB"
                     /locus_tag="PC472_001275"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2726609.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            36384..37076
                     /locus_tag="PC472_001276"
     CDS             36384..37076
                     /locus_tag="PC472_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2726610.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            37069..37971
                     /locus_tag="PC472_001277"
     CDS             37069..37971
                     /locus_tag="PC472_001277"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2726611.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKSRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            38056..38640
                     /locus_tag="PC472_001278"
     CDS             38056..38640
                     /locus_tag="PC472_001278"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2726612.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            38855..39781
                     /locus_tag="PC472_001279"
     CDS             38855..39781
                     /locus_tag="PC472_001279"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2726613.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITKVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(39833..40723)
                     /locus_tag="PC472_001280"
     CDS             complement(39833..40723)
                     /locus_tag="PC472_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2726614.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            40911..41942
                     /locus_tag="PC472_001281"
     CDS             40911..41942
                     /locus_tag="PC472_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2726615.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            42236..43111
                     /locus_tag="PC472_001282"
     CDS             42236..43111
                     /locus_tag="PC472_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF2726616.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYAEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            43129..44619
                     /gene="cls"
                     /locus_tag="PC472_001283"
     CDS             43129..44619
                     /gene="cls"
                     /locus_tag="PC472_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2726617.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            44812..45096
                     /locus_tag="PC472_001284"
     CDS             44812..45096
                     /locus_tag="PC472_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2726618.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            45129..46757
                     /gene="groL"
                     /locus_tag="PC472_001285"
     CDS             45129..46757
                     /gene="groL"
                     /locus_tag="PC472_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2726619.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            47019..47759
                     /gene="larB"
                     /locus_tag="PC472_001286"
     CDS             47019..47759
                     /gene="larB"
                     /locus_tag="PC472_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2726620.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASIINKL"
     gene            47843..48577
                     /gene="larC"
                     /locus_tag="PC472_001287"
     CDS             47843..48577
                     /gene="larC"
                     /locus_tag="PC472_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2726621.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            48607..49026
                     /locus_tag="PC472_001288"
     CDS             48607..49026
                     /locus_tag="PC472_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2726622.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILDRKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      49086..49187
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            49408..50943
                     /gene="guaA"
                     /locus_tag="PC472_001289"
     CDS             49408..50943
                     /gene="guaA"
                     /locus_tag="PC472_001289"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2726623.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(51020..52066)
                     /locus_tag="PC472_001290"
     CDS             complement(51020..52066)
                     /locus_tag="PC472_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2726624.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(52143..52358)
                     /locus_tag="PC472_001291"
     CDS             complement(52143..52358)
                     /locus_tag="PC472_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726625.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
BASE COUNT        20437 a         5938 c         8609 g        17730 t
ORIGIN      
        1 aaaaatccca tcttagaagt ttgtatcata tgaaaccttt atagattatg aaagcaataa
       61 tatagtgtat gatatataaa ataagttata aaagtatttt tttagatatt caaaaagaca
      121 ttgtaagtta tattaataat atgggtatca acgaattttg gatataatga gtttttcaac
      181 ttaaagttat gtaataatga atgtaaagaa tatttaatga aatcattttg ttggttatgt
      241 ctagctgaat acaataatct tttagataaa gattttatat ttgaagatag gtgtgaaaaa
      301 attaagaaga tattaacatc agcagtttag aggcaaagca tgtaaagtaa agtctgggta
      361 actaaaagta gtaatttaga ttgtaactat tgttataaat atgaaaatga ggaaacagga
      421 aatataagtt aggatgtaga aaataggtta ctaaataaaa agcttaatta tataatcaaa
      481 gaaataggta tatactgtac aatttcatgg tgaagaactt ttcctaaata aaaaggttgg
      541 taaaagagat ataagaaata aaattcaatt ataagatatg gtatgataac taaaggcact
      601 atatggagtg aataggcgga agaatttttt gaaaattata aaaatagttt ctgttgctac
      661 atatcaataa gtatagatag agattgcaaa atccatagca aaaatcgaat ttataagtgt
      721 aaataacatg taagacaagg agtaaattat gagtatttta gagataaaaa acctaaataa
      781 aatatatggt aaatcggaaa cagaggtgaa agcgttaagt caaataaatc taaaaataaa
      841 tagtggagaa tttgttgtga tagtaggtaa aagtggaagt gggaaaagta ctttactaca
      901 cataatggca ggacttgaaa ctccaacaaa tggtgatgtg attattgata atataaatat
      961 atcatctctt gatgaaaaga aaagatctgc attacgtaaa gaaaagttgg gattaatatt
     1021 tcaatcatat aatttgattc cagttttaac tgttgaagaa aatataaaac taccaactat
     1081 aaatataaaa aataaagatg aagcttatat taatgaattg atggagttac taggaataaa
     1141 agatagaaga atgtatttgc caaatcaatt atcaggtggg caacaacaaa gagtagcaat
     1201 agcaagggct cttgtaaata aaccatcaat tgtatttgct gatgaaccta ctggaaattt
     1261 agacacaaag acagagtcag atgtattaag tttattaaaa gaatctcaaa aaaaatataa
     1321 tcagacaata ataatgataa cacataatat agatataaca aaatatgctg atagagttat
     1381 agaaatagaa gatggattta taagagagta ggtagattta tgaaacttaa ttatattgta
     1441 aaaaaatata taatgggaaa taagaaaaat acattattaa taattgtaag tattgtaatt
     1501 tcaacagcgt tgtttttaat tatgaatata ataagtgaag atgcgacaaa cttaatgata
     1561 aatcaagcta aaaatgaatt tacattaaaa catgcacaat atatcaatcc aaaagacgaa
     1621 gaaataaaat atatagaaaa taacacaagt atagataaag ttggaaaatc aatgctttta
     1681 ggaattagtg atattggtaa aggtcaaaca ctacaaattc tatctcagga taaagttgct
     1741 gaagaactaa atgatattta cacactagaa aaaggtaaat taccagtagc tgaaaatgaa
     1801 atcgctatag attcatggta tattaaacaa aaaaagataa aaaaccctat aggaaaaagg
     1861 ataacattag attatagaag acaagggata gatcaagaag atttatatac tggggaaaaa
     1921 gaatttaaaa taactggtat cttaaatagt aatcctattt taaaagctca aggaacatca
     1981 attggcctta taagtaacga ttgtgctatc aagaatattc ctactaaaaa taaatatgat
     2041 caagttatgt ttacttttaa aaaggaaaaa aatatacaga gacaatcaca aaaacttatt
     2101 aaaaatggaa atttgaatga gaataacatt tattttaata atgaattatt aatagctata
     2161 tctgacagta tgaatttaaa aataccttat ataatagtta atatagtact agcactagca
     2221 actatattat taatttataa tattttttat atattagtat caaatagaat aaaggatttt
     2281 gggatattta gggctttagg gtttaatcca aatgatattt ttaaaataat gattttagaa
     2341 gtatctatat actctattat aagtatttct ataggtctaa ttttaggtgg gataatagct
     2401 agtttatcaa gggagtatgt tataggagta atatacaatg tcaactatat aaattctata
     2461 aaaaaccaga actatataaa cacatacata atttctatat tattaagttt aggaactata
     2521 ataatatcag tttcaaaacc tcttatgtta tcagttaaaa ctgatccaat gatttgtata
     2581 agaagaaata atgaaaaagt ggatataaaa caaaattcct ttataaataa atttatgata
     2641 aaacttttca aagactatgg taacatagca tcaaaaaata tacaaagaaa taagaaacgt
     2701 acaaatttat ctatagcatc aatggtaatt atatttttct taatggcaac aatatacaca
     2761 aagtctacta gtaacttttt aagtgatggt gtattaaaat attggataca aggagattat
     2821 ttattacata atattgatat aagtactata cgttcaaata ataaaggtta tgataaaaat
     2881 gtactacaag agataaaggc aattgatggt gtaacaaaag taaactcata taggcataag
     2941 tggtttaata taaaaattga tgataaaagt ataaataaga actctgatta ttggaaaaaa
     3001 aataaagata atattgaagc tagatcagaa gtaaaagatg gaattaaggt atataataat
     3061 tcttttgaat ttttaggaat agaagataca gatatactag aggatttctt gataaatgga
     3121 aaggaaaata tagataaatt tagtaaacaa ccatatctgt atataacaaa gaaatccagt
     3181 gattcgctta atataaaaaa aggagataga ataaaagtat actttgatat tatagattca
     3241 aaaacaaata attttaccaa aacaatatct caagaattta tagtatgtgg aattataact
     3301 acattaccag taacctcaca agtgggagct gaatttggcg gagtaatatc aacaaatcaa
     3361 tttaataagt ttacaggaat atcatcttat gaaagatttg acatatggac gagtaaacta
     3421 gccaatgata aatatgttga aagtgagtta aacaaaataa cagaaaaaac aaataaagga
     3481 attttaattc catacaaagc tgaaacagct gaatatgaaa aaattgataa tcaaaaaaca
     3541 ctaattatgg tattagtaac tggaattata gttatattat ctttatttaa ctgttgcaat
     3601 actatagtta ctagtattaa tagcagaagt agggaatttg cactatttag aggaatagga
     3661 atatcaaaac atgaaataaa taaaattgtt aaattggaaa gttatatata tattatagta
     3721 agcttttgta tatctataat acctatttta attgtaagaa gtgtaataat aaaacctttt
     3781 gaaagtatca atttaataaa ttggaaattt ataggggcta ttatcctaat agtatttatt
     3841 ttagtatcta taatcatgat aactacacta aaaacattga ataaaattca aaatgaaaat
     3901 tttatggaag aaataaagac tttatagtga acagcctata aattaaagag gttttatatg
     3961 aatttaattg caattatcga taatggtgta gatattgatt ttaaataatt tagaaataaa
     4021 aataaaataa gttatccaag ttatatgtat ggtgtaatag gggtacaatg tatagcaaat
     4081 ataaatattg gcttaataaa actctactct taagtctact gtcgttttaa cacaagacta
     4141 ataataagtg taacctaaaa attattgata atacaataac cttgtttatg ataaataaca
     4201 aatattaagt gttgtaaaga tagtaagttt aaaaaaatat caaagatttc aatcacaatg
     4261 tattgtttat ctgtagtgtt ggctatttat ggtatatatg tatttataaa ttgtactgaa
     4321 tatatatata ctatgctatc tacaaaacag ttaacagtca caggaaatga atatgccatt
     4381 ataaattatt atatgagtaa ctttagtcag tatataatat ttgctatttt acttgcatca
     4441 atgagatttg taatacaaaa gatagtgcat atgcatttca gtgagataga acttagtggt
     4501 aactattcaa caactgttag aaatataagt gatatctctt tagataatga taatgaaatg
     4561 tttaatagag gcaatattga aaatagtatt ttaaaaaaat aaataatata aatatataaa
     4621 atgtgattga ataaagctta gaatacttag tgtattttaa gcttttttta ttattaagat
     4681 ttcaaagtat taaattaact atataaaatc atataaaaat aattttaaaa tatttaaatc
     4741 attgatttca aggtattgta tttttaaaag agtaaaaata taataagtta ggtatataat
     4801 atatataaag taaaaaataa aaaaatggaa gtgattgaga tgagtagtat attgttggtt
     4861 gaagatgatt taagtttgat atatggatta gaatattcta tacaaaagaa tggatttagt
     4921 gttgatgtag caagaacagt agaagaagca ttgcaaattt ataaagaaaa aaattatgat
     4981 ttactgcttt tggatgtatc cttacctgat ggtgatggtt ttgatatttg taaaagggta
     5041 agagaagctt ctaatgtacc aatcatattt ttaactgctt ctgatgaaga agtaaatgta
     5101 gtgatgggac ttgatatggg tggagatgat tacataacaa aaccttttaa attaaatgag
     5161 ttaatttcaa gaataaaagc tttacttaga cgttataatt ttacaagtga aaatgtaact
     5221 gaactaaaat caaataatat tactgttaaa ttacttgaaa atcgtgtttt taaaaatgaa
     5281 attgagcttg agcttactac agcagaatat aaattattat gcttacttat gaaaaataaa
     5341 aatattgtac ttacaagaaa aaatatattg gataagttat gggatggaaa tggtagtttt
     5401 gtagatgata atactctgtc agtatatgta agaaggttaa gaaataagat tgaagataat
     5461 cctgaaaatc caaaaattct attaacagtt agaagaatgg gttacaaatg gaatgttata
     5521 aagtgagggt gttaatatga aggtattttc taataaagac ataaaaatat tttttttggt
     5581 gattatatcc gttttattga tgtttatagt agtaggtcaa atagtagtta taaatataac
     5641 taatgattat aaaagtgcac tgttagaaca tgattataat atagctggat acttaaatag
     5701 tatgggtgtt gataaatcaa aaataccagc agtatttact actaatgaag ataaatatac
     5761 tttaaaagga aaagaaatct taaatacagc tagatatgac tttggtacag atgatagttt
     5821 tatgccaagt gtgaaattgt ttcaaggtaa atatataaaa actatgtttt tgtatttatt
     5881 tgtgttcttt actatgatta tttttatatt gtatgtgtat ttttttaggc aacagaacaa
     5941 gatagaagaa gctagttata aaattgattc atttatgaat ggaaatataa atgtacggtt
     6001 aaatacttat gaagaaggta gtttatcaaa gttatttggt tcaatagatg ctatgtcaac
     6061 atctctaaac acacatatat tgaaagaaaa gcagaataaa gaatttctta aaaatacaat
     6121 ttcagatata tcacatcaac taaaaactcc acttacagca ttgatgatgt ataatcaaat
     6181 attgcaggaa gagagtcata atagtgaaat tgttgagaat tttgtgcaga agagtcaaaa
     6241 tgaattagag cgtatagaat cattgataca aaacttgtta aagattacaa agatagattc
     6301 aaagacaata attcttaaca aagctagtgt taacgtaaaa aagttgatta ctaaaatttt
     6361 atatagtttt gaaactagag cagaaaaaga aggaaagagt atctctataa gaggtctaga
     6421 aaatacaaac ttgttttgcg attatgaatg gatatcagaa gctttaagca accttataaa
     6481 aaatgcatta gataatacaa aagagaacga taaaataatg atagaatggg ttgaaacacc
     6541 tataacaact attatttcag tgagtgatac cggaaatgga attcatattg aagatataca
     6601 tcatatattt aaaagatttt ataggagtaa gttttcaaaa agtaatcaag gccttggatt
     6661 agggttgcca ctagttaaat ctatcataga acagcacaat ggaaccataa ctgtagaaag
     6721 taatttttta caaggaagta catttactct aagtttttta aagcttacta atatgtaaga
     6781 taggatacat cttacagtaa ggtgtatgaa ttattcttat attaatcatc agaaaggaga
     6841 tagagcaaat ggatattctt gtggttaata atttatgtaa aacatatgga aatggatata
     6901 caaaagtaga ggcattaaaa gatgtatcat ttacagtttc aaagggagag tttgttactg
     6961 tagttggaga atctggctca ggtaagagta cattattaaa tcttttagga ggtcttgata
     7021 ctccaacctc aggaaaaata tttgttgatg gtaaagaaat atgttcaatg aaagaagaaa
     7081 aacagactat ttttcgacgt cgtaatatag gttttatctt tcaatcatat aatcttatac
     7141 ccgaattaac tgtggaacaa aatataatat ttcctttact actagattac aaaaaaccag
     7201 acactattta tctagatgaa gtattgacaa ttttaggtct taaagataga aaaagtcatc
     7261 ttccaaatca attatctggt ggtcagcaac aaagggttgc aattggaaga gcattgatta
     7321 caaagccatc aattatattg gcagatgagc caacaggaaa tcttgacagt aaaaatagca
     7381 gtgatgtaat ttcactttta aaagtgacag cagaaaaata taaccaaact attattatga
     7441 taactcataa tacaaatata gcttctacag cagataggat actacaggtt tctgatggaa
     7501 tgcttaaaga cctaggggtg aatagagaat gagaagctat ttagaactag taccacaata
     7561 tgcaaaggta cataagaaaa aaaatagaac tacaatttta tgtattgtca ttgcagtatg
     7621 tttagttact gctatttttg gactagcaga catggctata caatgtcaaa aagtacaatt
     7681 tataaaagat gatggaaatt ggcacatagt acttaaaaat cttaatgaag aagctatatc
     7741 agaaataagt agtagagtag atatagctca ctatggtaga ggacaagtca ttgataatgg
     7801 agagtttaaa tcaaaggaga ttttgataca aggtttagat gaagatttag caaaacaatt
     7861 aggtctagaa ataataaaag gtcgttatcc tgttgtggag aatgagactt taatagatgt
     7921 agatgcagtt aaaagcttta atctaaaagt aggagatttt atcaaaataa aattagcaga
     7981 tggtagaaat aaaaattata agatagtagg tgaatattca tcattatcaa gcttaagagc
     8041 ctcagataag catggattaa tgctttcaat attaggtaca caagctatca aaaaatcaga
     8101 aacatatgat tattattata ttacatttaa agacaaggtt aatattagaa aagctacttc
     8161 tgatattaag aataccttca atttaagtga taaacaaatc ggtaaaaatg aaaaattact
     8221 ttctattata ggtcaaggtg actctgatta ttctaaacca ctttacttaa cagcaggagg
     8281 actttttata cttgtgttaa tagcaagtat tatcatgata tataatagct ttaatatggg
     8341 tgtaatagag agaataaagt tttttggact attacgttgc ttgggagcat caaaactcca
     8401 agtcaaaaaa tttgttgttt tagaaagttt aattctaagt gtaaaggcta ttccactagg
     8461 attgttatta ggatgtattg taaccattat atcctctata tttctcaaat atgtgaatac
     8521 agagttattt ggttcgatgc caattttaaa ggttagcttt attggtattg tgtttggaat
     8581 aatagtagga tttttaacag taatattatc agctattgtt ccagcaaaaa aagcttctgg
     8641 ggtgtctcca ctaagtgcta tacgtggtaa tctaatgttc aaagaaattt taactagtgg
     8701 aaaagcaagt aaaattgcta acgcaggaag ttctaaaatt gatgtttcta tgggaataaa
     8761 tcatgccttt tcaagaaaaa aatcttttat acttatgact tcttcatttg ctattagtat
     8821 tgtattgttt ttaggtttta gtgtatttat agactttatg tatcaggcac ttaaacctat
     8881 aaaaccagaa gctgctcata tatctattgt tgacgctgac tttaagtctg ttcttactaa
     8941 agacgatatt aatgaaataa gtaatatcaa aggaataaaa aaagtataca gtagaatgat
     9001 gacggatgta gaggctacat acaataataa ggaggcaaca tctcagctaa tttcttatga
     9061 aaaaaatcaa tttgactggg ccaaaaagta tctagtaaaa ggggaaatca atgaagataa
     9121 attagagtca gaaaatatcg gattggtaga agaaggtcat gggtttaata ttggagataa
     9181 gatatatatt aaaaatggaa attctaagaa agagatagag gtagcaggta tactttcttc
     9241 aatttcattt gatgttaaag acaaatatat tggaaatatt ataacatctg aaaatacttt
     9301 tactagtata acagatataa aagactatat aataattgat actcaagttg atgaagatgc
     9361 tcctgaagat ttaatccaaa atataagaaa tgttctagat gataatttag aggttcgaga
     9421 taagcgacaa ggtaacttag aggctaaaaa ttcattttac actatggcag tttttgtata
     9481 tgggtttgta tttataattg cgattataag tatgtttaat atcataaaca gtatgaatat
     9541 aagcgttaca agtagaataa attactatgg tattatgcgt gcaataggca tgtccaataa
     9601 gcaattaaga aaaatggtaa tagtagaatc aagtacatat gctgtaagtg gctgtttatt
     9661 ggggagtgta cttggattaa tattacatag atatatattt gtatccttag taacattaaa
     9721 attccatata gactggcaaa taccttttga tttactgttt atcattgtag taactatgat
     9781 aataataact ttgcttgcag taagaaaacc aattaaaaag atttgtgaat tagacataat
     9841 agaggtagta aatgcacaat aaatataagt agtaatttaa atagaggtag taatttaaat
     9901 aaaaaatcga agtaaatatg aaatagaaaa tatctttcat aatttacttc gatttttgtg
     9961 ttatataatt tactttagat aagttccatc ttcatcttga taaattaagt ctttcttcat
    10021 aagtactcca agagcacgtt taaaattttc ttacttgaat taaataagat agaaatatct
    10081 ttaggatcag atttatcatt gaatctcata tatccatctg aaccttctaa atagcttaaa
    10141 atcttatttt ctaaggtatc aagttcatct tttctgtttc ctctaggaga aagtcctaat
    10201 ttaccatcat cgtatatttt tataacatgt aaattttcaa gaacatcccc agcttttaat
    10261 tctgtaaagt attcattatg aaggattaca ccagcatatt tattgtcaac acatatcata
    10321 gcagagttgt tggtttggaa tccatataca actccattta caacatctcc tacattgtaa
    10381 gtatggtctg tttctaaata tgaatctatg tctgtagtag cagctattct tctgctttta
    10441 tccaagtata tatagaataa atatttttca tctctaaaca atggataagt cttagcttta
    10501 aaaggaacta gtatgtcttt tgtaagtccc atatctataa aagtaccaat atccgtgtta
    10561 tcaactactt ttagatacgt aacatctcca tttctaatta tttctatagt attagaaaaa
    10621 taatttttaa atagtttgta aaatattatt gaattttttc tctcgaaata tataatttag
    10681 aatcttctga tttattttta tgctcgattt ccttattttt tttcataaat atatatagag
    10741 gagctattaa caacattgcc cctaatccta ttaaaactct attttgagtt ataggttttg
    10801 aaatatcttc tgcccaagct tgttgtaaca accataagct acctaaaata gctaccattg
    10861 gtataacagg accaaatgga atcttaaaag aggccttaac atctgttctt tttcttaaaa
    10921 ctattactga taaacaagtt ggtatgtact gagcaaatct tgcaattaca ctcattactg
    10981 caagttgttc aaaacttcca gttagcacaa gtggtataca acaaattaaa gatattatta
    11041 ttgctacata tggagcacca tatttatttg tttttcctat aaatgcagga agagaacctt
    11101 cttctgctag agaagaacca catttaggag ttactattga tgaacctata tttataccaa
    11161 atatagatat taatgttgct acagatatga aaactttacc ataattgcct aaaaatacac
    11221 ttgaagcatc tgcaattgga atactgtttt caaatagtct atctccaagt atacccatac
    11281 agacaatttg aattaatata taaattattg cacatataaa tattgtggtt attaatgcga
    11341 ctggaagatt ttttttagga ttttccattt ctccacttgc cacaataaat gattcaaagc
    11401 cggtaaatgc gtaaaatact aatataatgg ctggacccat actagagtta actacatttc
    11461 ctgaaggaac aatatttaca aattttatga agaatattcc aactattata aaaactatta
    11521 atggtactaa tttagctata gttattacat tattcattat ctttgtgctt ttaattccaa
    11581 ataagctatt tattgaaagt agtgttacga gtattgcagc tatataacct ttgtattcag
    11641 tggcagattc tggccaaaat gaacctaaag ctgttgcaaa tcctactgct agagtagacc
    11701 atgatataat tctaataacc caagacattg taccaacttc aaatcctata aaatctccaa
    11761 aagctttttt ggagtataga tatgctcctc cattcttatc aaacatgctt ccaacttctg
    11821 caaaacataa aagtattgat agaactaaga gtgtagcaaa aatgtatata aacatactgt
    11881 tttgacctgc tagattataa actttacctg gtagtaagaa tatacctgaa cctattatgc
    11941 tattaatccc taataatatc atactaaata gacctaattt cttagctttt tccatattaa
    12001 tttatctcct attcttttat taattactgt actaattaaa atacaaagta ttcattacat
    12061 gcatcataac acctattggt aaacaatctt catctatatt aaagctagag gtatgaagag
    12121 gtgaaattaa gcccttttcc tcatttttac atcctaaatg gaagaatgct cctttacaat
    12181 gttctgtgta aaaagaaaag tcttcaccac ctaaagacgg gtttggtctc aatataaatt
    12241 tttcctctcc aagtaattct ttagtatttg atataactgt atctactaat tctttttcat
    12301 ttataacagc aggatagttt tcatcactta catgtaaagt cccaacacaa ccaaatgcag
    12361 atgcagtgtc tccaacaatt ttagctattt tatcaatcat aaagtttctt gttttgctat
    12421 ttaaggttct taaagtgcct tctagtttta catcttcaca tattacattg tgtgcatcac
    12481 caccatatat tttacctata gtcaaaacag cagcattatt tggttctaag tttctactta
    12541 ttatagtttg taatgatgta acaatttgtg aagctgtaac tattgcatca ataccatttt
    12601 caggatatgc accatgagct cttttacctt tgatacttat ttttatagtg tctacactag
    12661 catttaatgt atcatatttt gtttcgataa atcctgtttc gatatgtggc ataacatgaa
    12721 gtccgaatat ataatcgacc ttaggatttt ctaaacatcc atcttcaact aagaattttg
    12781 ctcctccaaa accttcttct gctggttgaa ataagaattt tacatttaca ttaagtttat
    12841 cttttattga atgtaaaact ttacatgctc caagtagcat ggttgtatgt gcatcatggc
    12901 cacaagcatg catttttcca gaatgtattg atttataagg tatgttgttt tcttcttcaa
    12961 taggaagagc atccatatct gctcttatac aaatcgtttt atctgcactt tcttttaaaa
    13021 tatatgccat aattccattg tgttttgtaa attccatata atcaattccg atttctttta
    13081 aataagaaat taccttctgt ttagtaagat tttcttcaag agctaattct ggaattttat
    13141 gtaattctct tcttatgttt ataagccatg gttttataga attacaactt tctattacaa
    13201 aatcttttcc taattctttg gtttgatatt ttaatttcaa agcctgttgc atttttattc
    13261 ctccaattgc tttttattgt tttattaata taataagcaa ttattatgcc ataattttcg
    13321 atggagaatt ttgtttttta taagaatatt gaataaatat aatcatttgt ctatatgtat
    13381 agtataaaaa atgattgcat agtttcaatt ttcagtaaaa aatattattt tgaagaaaat
    13441 atattttata tgctagttta tataaaaaaa ataggaaata aaaaaattat cctattccta
    13501 ttaattccta ttatttatgt acaaaataaa ttataatttt agggtacttg aaaaataaaa
    13561 aaacctattg aaggagtact ttaatcctga atagatttct ttgtttgata aattataatt
    13621 ttatagacct ttatatttta atttattgta aaaatcttct cctttttgag ttattttact
    13681 tccatatctt cctgaactac aaattataag ttcttctttt tttagatatt caaatatttt
    13741 tttaattttt ccttctgtaa tttgaaggtt cttatcgagt agtgctttta aaattaatcc
    13801 tctaccaata ccaacatctg attccttatt taaaattagt atctctaata tattcaatat
    13861 atcattctta ctatatttta gttttaatga tgtatttttg ttatctgaag accttaagtt
    13921 tggaggtaaa tctttctcta atacaatatc ttcacacata atgtttaaat aggatgtaac
    13981 attttggagt tctcttatat tacctttcca catatagttt tgtataagtt tttttgcttc
    14041 tggtgtgatt actaatttct tattaattaa atcttccata ataattaata tgtcttcttt
    14101 tctttctctt agaggaggta tatttattgg aattgtattt aatcgataat ataaatcctc
    14161 tctaaattgg ctattatcaa tcatttgttc taaattctta tttgtagcag atatgattct
    14221 aacatctata ttaattacat tgtgagatcc aacaggcatt atctgctttt cttgaagaac
    14281 tctaagtagt tttgtttgta attctaaagg catatcgcct atttcatcta agaatattgt
    14341 tccattatta gcaagttcaa ataatccttg ttttccatct tttagacctc cagtaaaagt
    14401 tcctttgtca tatccgaata gctgactttc taataaattt tcaggaacag ctgcacaatt
    14461 tactgctata aagggttgat tttttctatt tgagttgtta tgtatagact gagctatgag
    14521 ttctttacca gttccacttt cacctctgat aagtatgctt aagtcagatt tagaaacctt
    14581 cttagctaaa tctatacatt caaacatttt aggactattt gtttttatat ccttaaatgt
    14641 atattttgct atttgtcctt tttctcttaa ctttttagta agattttgtt ctaatttttt
    14701 tatgtatgtt atttcttgta aactataata tgttcccatt ttttcatctc tattgtatat
    14761 gtttttttta tttacattta tatatttcct attgaatacg acaactttat ctagtatctc
    14821 tttctcactc aataaaactt ttaaactatc tacaaatata tcctcaatgt attttccata
    14881 tatatcctca tttatgtcta aaatatcttt tactttacta ttatatgtat ttatttcacc
    14941 atcttttgaa gtaaataata taccatcttt tgataaattc aagattgtat ctaattcttc
    15001 tatttttaaa aataattcct tatatttatc ttttatacca gtatctaaac ttattatttc
    15061 ttctgagtat ttaactagat tagattgtat ttcctttgaa tcaatttgta ataaatttat
    15121 tatttctata aatgtagaga tatctatata tctgtgacct aaatcaaata tatctgagat
    15181 aaagctaggt actttttctg gaactcctgg agttatagct atttttatat ttttatagtt
    15241 attatcattc atatatggaa ttggtctaat attagtcact ccaattttat aaaataaact
    15301 tatagtttct agagtagttt catcagaatc atttacgatt agaacgtttg taccctgtgg
    15361 taaagatagt agattataaa ttttatcttc cctaaaagtt cttcttacca caatcacttt
    15421 tttcttatct agtatattat ttataatttt gtctaatctt tctactgaca ttactaatat
    15481 cacatcatca tttataatat cattatcatt tatttcattt atgaagtatc tattaatatt
    15541 tatagaatct ccaaaaacta atttcaaatt attttctaaa aagtctatta aatcttttct
    15601 actatcattt accagagcta ctgatttttt catttatttc tccttatata ataaatagtt
    15661 tccaacaaaa tagttgtata aagcaatata attttgataa tttaaattta tcaaataaaa
    15721 aagaaagtgt ctataatgac tttataaaag ttgcattaag acactttctt tcttataatt
    15781 aaaaatcttg tcaccaaatc cccctaattg ttgcttaaaa cttaaactgt atgtatattt
    15841 ttcatttcta ttcgattaaa ttataatata cacccaaaaa aatttatttt aataaattct
    15901 attgataata taattttgta aatatagaat tatattaaaa taaattatat atgaaaatat
    15961 atatgtataa gtttattctg cgtgagtaga atgaatttgt caagtttaat ttagttgtaa
    16021 ttttatcttt tattaaaaat aatataaatt aaaacatata aataaaatat tttatacaat
    16081 atattgtaaa ggaggtaact ttgatgaaag cagaaaaaac aagtatatat ccaattatta
    16141 aaaataattt aaacaaaata tcaattgttg aatataaaaa gggtcaaaga tttgtagcat
    16201 cagataaaga cttacaagaa gtgttcttta taatagaggg agttgcatta gtggagtgta
    16261 ctactagagg aggaaataaa tttcttgttg acattgtacc tgaaaatgag tttataggca
    16321 aaattagtta catatatgaa cataatttga aatgtgatat atttgcaaag actaatataa
    16381 aattatttcg atttgaaaaa aatgtatttg aagagtttta tctcaaacca gattttattg
    16441 cactatttca cagaaaatgt acaagaagaa tatatgaatt atataaaacc agaatggtaa
    16501 gagaactatt ttcttgcaca gaagttattg cctattgtat atcaaataaa caagaaaata
    16561 atgtctgcaa tataaaagct attcgtaatt tcagtgaaat ctattcaatt agtagaaaaa
    16621 gtagatacca ttctctgcta aagttaactg aaaaggagat agtaaaaaaa atgggtaata
    16681 catatgaaat tttaaactat gatgaattac ataaattggc atttgaagta aaagaatttt
    16741 tggaagatga gtaaaaattg ttacttaatc aaaataaagt ataataattt ctgtaaaaaa
    16801 tagaaatagt ctcactgaaa atcaaaaata ctttagttat tttcaatgag actgtttaga
    16861 aatattgtat acttataatg tatagtataa ttattaatca atttccttta ctatattaat
    16921 agcccatttt ttagataatg ctctaggaac accaaatata aacttaacca cttcttcact
    16981 ataagtgagc aaaacgagta caaatatagg tgcaccttta aatgcagcta taaaagttag
    17041 aggaatagaa gctaaccaca tacatcctaa ttctaaaaat agagcatatt tagtatctcc
    17101 tccacttctt aaaataccaa ttataaccaa tgtattaaaa gatttaagag ccattagtat
    17161 tcccattata aagaatatct tagttatatc agaagctaag ctatcagaaa cactaaacat
    17221 ttttaataat aatggtatat ttaaaattaa aataattcca agtattaaac cagctgaaaa
    17281 tacaagtata gaaaactttt tagcatattc aatggctctt ttaatatggt cagctccaag
    17341 ttcatttcca agcataatag atgcaccaga tgcaatacat acagctgtca ttatgaagaa
    17401 attacctgta ctggttgcta tctggctagc tgctatagca gaagtaccag ctcttgcata
    17461 tgcaacagaa tacaggacag ttccaattgc ccatacagag tcatttacaa atataggaaa
    17521 gctttttgat gaaaaagatt ttataaaaat tttatcaata gcttttagat tttttagacc
    17581 aaactttaat atatagtctt ttttataaag gtaaacataa gtaatcatta aaattaattc
    17641 acatattcta gcaataacag tagctagagc agcacctttt acacccaatg caggaagacc
    17701 gaagttacca aatataaatc cataatttaa aataacattt gttacaagtg caaatgcact
    17761 acaaatcata cctaacttag gatttctaac acctcttgaa ccagtactaa atacagtact
    17821 tacagcaatt aatggataac taaatactgc aatactaaag taatctatac aaagttttac
    17881 cacctcagaa tcatacgaaa atatgtgtat aattggcttt ggtgttagta aagcaggaat
    17941 aacaaataca aaacttaaaa caattgccaa tacacaagta agacctgtga cttttcttat
    18001 attactcaca tccttctttc cataaaactg tgaaataaaa actccagcac ctcctgtgat
    18061 accagataag gacatattaa aaagaaagaa aaattggttt gcaactccaa ctgaagctac
    18121 agatgtttcc cccaaactac ttatcataat tgtatctatt acatttacag aagtagaaat
    18181 taagttctgt atgataatag gtatacataa agatagaagt attttataaa atcttttatt
    18241 ttcgtctttt aaagaaaata gttcttgcaa ataatcctct cctttttaca taaattcttc
    18301 ccactcatca tatagagagg caaggttatt ttctaaatta attttttctt ggctaacttc
    18361 tttagcttta tctggattag agtatacttc ttcttgacat aaaagtatat ctaatttttc
    18421 aatttcaatc tctaactttt caatttcttt ttctatattt tgtcttttta ttctattttt
    18481 cttttctatt tctctttgtt ctctctcttt tcttttttca tcttttattt gagttctagt
    18541 tttttcttct ttttcttcta taatactcat ctcttggatt tgcctttttt tatctatata
    18601 ataatcataa tttcccagat attcagtaat tccattttca tctaaaacta aaattttatc
    18661 aacaacagta tttagaaagt atctatcatg tgatattgta aatatagtcc catcataatt
    18721 agttaatgcc tcttcaagga cttccttaga atcaatatct aggtgattag taggctcatc
    18781 taataacaat aagtttgcat ttgatagtat aagtttaagt atggcaactc tagctctttc
    18841 tccaccactc aatgtagaaa tcttcttaaa aacttcttca tctacaaata aaaatgcccc
    18901 taacatattt ctcaaagtag tttgagtaag atgagtatta ttttcccaaa tctcatcaat
    18961 tattgtgtta tctaaattaa gagttttttg ttcctgatgg aagtaggaaa catgtacatt
    19021 agttccaaat ttaatattgc cactaagagg ttgtagttca ttagttatta ttttaaataa
    19081 tgtagatttg ccaacaccat ttgcacctat aagtgcaact ttttcccctc tatatatatc
    19141 taaatccaag tctttaaaaa gtattctttc tccataaccc atagatacat ctctaatttg
    19201 aagaacatca ttaccactct ggacagaagg cgtaaattgt atacgagctt ttttcctata
    19261 agcttctggt ttatctaaaa cgtctacctt gtctaatgct ttttctttac ttcttgcacg
    19321 ctttaggtgt ttttctctac cataagcttt tagtctttca attgattctt cttgcttttt
    19381 tatttctttt tgttggtctt caaatttctt aagctctagt tctttttcta ttttagataa
    19441 ctcaacaaat ttagagtagt taccattata tactttaagt tttttgttgt gaacttcaaa
    19501 aattctattt acggattggt ctaagaaata tctatcatga gatattaaca tgacagtccc
    19561 tttatattgt tttaaaaata cttctaacca ttctattgct tcagagtcta agtggttagt
    19621 aggttcatca agtaacaata gagttggttt cttaagaagt aattttccta aaagaactct
    19681 agttttttca ccaccagaaa gtatgcttat tggcttatcc atatcattgt cactaaatcc
    19741 tagtcctttt agaactcctt tggcttcaga tttatatcca tatccattta aatctgaaaa
    19801 taattctagt ttatgtgaat attcatccat gattttttct ataattggag agtttgtttt
    19861 tgtactttct tcagatattt taacctccaa ttctcttaaa taggattcca tttctatcaa
    19921 atttttaaaa acttctagta cttcttccaa aatagtatta tccgaataaa aattggtatt
    19981 ttgttctaaa tatccgattt cacaatcttt agaagtgtaa atatctccac tatcatatcc
    20041 ataaattcct gaaattattt taaataaagt agtttttcca gtaccattaa caccgattat
    20101 accaatttta tcgccttcat ttactgtaaa actaatattt tctaatatag aatctatacc
    20161 aaaactttta tttaagttat tacatgacaa aacaatcata aatttctacc tccttgaact
    20221 atctaaaatt ataatgtttt gatataatat aatcaaggtt atatgtatta gttttgaaaa
    20281 agaaatattt tattgtgaat caaacaaaaa agaggacact tttaggaggt gaagatgttg
    20341 ggaaataaaa atatatcaat ggcagttata agaaggctcc caaaatatca tagatatctt
    20401 ggagacttat tagataggga tatacaaaga atatcttcta aagaattgag tgatataata
    20461 gggtttaccg cttctcaaat aagacaagat ttaaacaact ttggtggatt tggacaacaa
    20521 ggatatggtt ataatgtaga agctcttcat actgagatag gtaaaattct tgggttggat
    20581 cgaccataca acgcagttct tgtaggagca ggtaacttag gacaagctat agccaattat
    20641 gcaggattta gaaaagctgg attcgagata aaagctttat ttgatgcaaa tcctagaatg
    20701 ataggtttaa agataagaga gtttgaagta ttagattcag atactttaga agactttata
    20761 aaaaacaata atatagatat tgctgtatta tgtataccta aaaatggagc acaagaagtt
    20821 attaatagag ttgtaaaagc tggaatcaaa ggtgtatgga attttgcacc tttagattta
    20881 gaagttccga aaggtgttat agttgaaaat gtaaacttaa cagaaagttt atttacctta
    20941 tcgtatttaa tgaaagaagg aaagtagaca acttaaacat attaataatg agcagttgtg
    21001 aagagtattg agaaagtcag aagatttaat attaaatcct ctggcttttt gaaatattag
    21061 gataaaaatt taaataatgt atggaaataa acaatattaa ggagagtggt ttttgtggag
    21121 gaattatgga aaaaggcaat taaatttcat ggtcatgaat gtccaggatt agctatagga
    21181 tttaaagcag cacttgcagc aagagaatat ttaggatgta aacagtcagg agatgaggaa
    21241 attgtatgta tttctgaaaa tgatgcttgt ggaatagatg gtatacaagt cgtgctaagt
    21301 tgcacaatgg gtaaaggaaa tttactactt aggatgactg ggaaatcagt atatcatttc
    21361 tttaataggg aaaatggaaa atctataaga atatatgtaa agtcaaagcc atttcaaatg
    21421 gatagagaag gatacaaaaa gtacttatta gaaagtgatt ttgaagaaat atttgagatt
    21481 acagatacta agttaagact tcctgaaaaa gcaagaatat ttaaatctct taaatgtgaa
    21541 gtgtgtggtg aatttgcatc tgagcaatat ataaggattg aagaaggaaa gaaagtatgt
    21601 cttgattgct ttaataaata tgaaagattt tatgaggagt attaaaatta taaattagat
    21661 taaagttaaa agtattagat atgtgctaac agtattttaa gatgaattag gtagcaacat
    21721 aataaagtat ataaataata ttaattgaat catacataat taaaaagagg ctgattagta
    21781 tttataaaat aaaatcagtc tctttatgaa taacgaatta ttattctggt tgaggtgcat
    21841 ctacaggaca agctcctgca caagaaccac agtctataca agaaccagca tctataacat
    21901 atgcgtcgtc tccagctgat atacagctaa cagggcattc agcctcacaa gcaccacagc
    21961 ttatacaagc gtcagtaatt ttatatgcca ttttgaaatc ctccctttta tatgtattgt
    22021 aattcaataa cattataaca aatattattg aatataacaa ttaaattaat tctacaacaa
    22081 aaactttatt atgatattct tgttttaaag ttttacaata aagtttaatc tgcttatatt
    22141 tttatttatt ttaaagaagt ttattttaaa aataaaatta aatttaaaaa gttatgtatt
    22201 gattatattt aaaaatgtta tactctaaaa aagtatattt tatttaaaaa tttattaata
    22261 caaaggagat ttactatgaa aaataaatta gtttacatat gtattatgag tctattgcta
    22321 tttgtagtag gatgttctaa taatccaaat aaggaaagta ccattaaaaa agactctaag
    22381 aataaccaaa gccaagttaa agaagaaaaa agagaacctg tgaaaaaaga tataactata
    22441 agtttcgctg gtgatgtaac tatggggaac tataaaggtt catcatatta tggcagtttt
    22501 gaccatgaat ttaaaagaca aggagaagat tacaattatt ttcttaaaaa tgtaaaacct
    22561 gtatttgaaa aagatgactt aacagtggtt aatttagaag gtccacttac tacagcgtca
    22621 aatgcaaaag aaaaaaaatt tgcatttaaa ggaaagcctg gatatgtaaa tattttaaaa
    22681 tctggaagtg tagaggcagt aagtattgca aataatcatt cagaagatta ttttgaagaa
    22741 ggaatgaaag atacacagtt tatattggac gagaataaaa taaattattt tggattaggg
    22801 aaagatgctg ttgtagatgt caaaggaatt aaagtgggtc tattagggta taatggatta
    22861 tcaactgaat ataatgaaaa aaatttaaaa caaatggaag atgatattaa gtccttaaaa
    22921 aagaaatcgg atgttgttgt agtgtacttt cattggggga tagaattaga ttattatcca
    22981 gataaaaaac aaaaagattt tgctcattat gctatagaca agggagcaga tttagttatg
    23041 gggtctcacc cacatgtgat tcaaggaata gaaaagtata aaaataagta tatagcttat
    23101 agtctaggga atttttgctt tggaggaaat aagaatcctt ctgatacaga tagttatata
    23161 tatcaacaaa cctttacttt ttcagataat aaattaactt caattaaaga gccaaatata
    23221 ataccaactt caataacatc tagtaactcc agaaataatt atcaaccaaa aatattggat
    23281 ggaagtgaga aggatagagt tttaaataaa cttgaaaaaa taagcaaaga tttaaataaa
    23341 taaaatgtaa aaaaaaataa atatatatat ttattttaat aatttatatt ttgtttaggg
    23401 tatggtaaat cgtggtatta aataagtaga ttttaatagt taaattatag tcaattataa
    23461 taaagctatt taatctactt ttttaatata aaaatataag ggggaatttg taatggcttg
    23521 taacacttgt aaaatgtgcg aaagtgcaga caagaaatta gaaagttttg tagcttcaaa
    23581 ggatgttgaa acagcatttc atagaacaga agatcagaaa gttaagtgtg gttttggact
    23641 tcagggggtt tgttgtagac tctgttcaaa tggaccatgt agagtaactc ctaaatcacc
    23701 aagaggtata tgtggagcag atgcagacac tatagtagca agaaactttt taagagcagt
    23761 agcatcagga gcagcttgtt atttacatgt ggtagaaaat acagctaaga atttaaaaaa
    23821 tgttggaata actaaaggtg ttgttaaagg agaaaaaaca ttaaatcaat tagcagagat
    23881 gtttggaata gaaagtgatg aaaagtatga taaatgtata aaggttgcag acaaagttat
    23941 caacgatttg tataaatcaa gagatgataa aatggaatta gtagaaaaaa tagcttatgc
    24001 acctagagtg aaaaagtgga aagagttagg tataatgcct ggtggtgcaa agtcagaagt
    24061 ttttgatgca atagtaaaat catcaacaaa tttaaatagt gacccagtag acatgctagt
    24121 taattgtttg aacttaggta tatcaacagg attatatggt cttacactaa ctaatctttt
    24181 aaatgatgtt atgttgggag aaccagttat aagaatggct cctgtaggat ttaatgtaat
    24241 agacccagac tacataaata taatgataac aggccatcaa cattcaacat ttgcaaattt
    24301 ccaagataga ttaaaagatg aagatgttat aaaattagct caatctgttg gtgcaaaagg
    24361 ctttaagcta gtaggatgta catgtgttgg tcaagattta caattaagag gagaacatta
    24421 tcaagaagta tttgctggac atgcaggtaa caattttaca agtgaggctg ttttatcaac
    24481 tggagcaata gatatagttc tttcagaatt taactgtact ataccagggc ttgaaccaat
    24541 agcagataaa tataaagtaa aaatggtttg tttagatgat gttgctaaaa aagcgaatgc
    24601 agaatacata ggtttagaca gaagtaaatt agatgaactt agtaacactt taatagaaaa
    24661 agcattggag tcttataagg agagaagagg aagtattgaa atagatatac caaaagacca
    24721 tggatttgaa caatctttaa ctggtgtaag tgaaaagaac ctgaaagcgt ttttaggaga
    24781 ttcatggaaa cctcttatta atttaattgc agaaggaaaa attaaaggtg ttgcagctgt
    24841 tgtaggatgt tcaaatatga cagcaggagg acatgatgta aatactgttg aacttactaa
    24901 agaattgatt aaaaaagata taatagtact atctgcagga tgttctactg gaggattaga
    24961 aaatgttggt cttatgtcac ctggagcaga agaattagca ggagaaaact taaaagaagt
    25021 atgtaagact ttaggaatac caccagtatt aaactttgga ccatgtctag ctataggaag
    25081 actagaaata gtagcaacag aattagcagc tgaacttgga attgacctac cacaattacc
    25141 attagtgctt tcagctccac aatggttgga agaacaagca ttagctgatg gagcgtttgg
    25201 tcttgccctt ggcttacctc ttcatttagc tttacctcca tttataacag gaggaaaatt
    25261 ggtgacagaa gtattaacag aaaaattaaa agacttaaca ggaggacatg ttatagtgaa
    25321 tccagaccct aaatcttcag ctaatcaatt agaagaaata ataatagata gaagaaaaaa
    25381 tttagggtta aaggatgtgg aatgcaatgc atagaatttt tataaataaa gatttatgta
    25441 caggatgtaa gagctgtgta ctagcatgta tgctaaaaca caataaagat tatgacatgt
    25501 acactcttga tttagaaaat atagataatg atagtagagg gcatatagaa ttagatagta
    25561 gacataataa tccagttcca attctttgta gacactgtga tgaacctgaa tgtgtattag
    25621 catgtatgag tggtgcaatg cataaagata gtgaaagtgg aatcgtttca tatgatgaag
    25681 agaaatgtgg ttcatgttat atgtgtgtaa tgtcttgtcc atatggatta ttaaagccag
    25741 atgatagaag caagcagaac atacttaaat gtgatttatg taaagatgag gagtatccta
    25801 gatgtgtagc taattgccca agtggagcaa ttgaattaca gaaggaggaa aatgatgaat
    25861 tatgtagtgt taggggctag tgcagctggt ataaatgcag taaaaacatt aagagaatta
    25921 gataaagatt caaatatagt agtaatttct aaagatgaaa acgtatattc aagatgtatg
    25981 ttgcatcacg ttatatctga acacagaact ttaaaacaaa taaattttgt tgatgaagat
    26041 tttatggagc aaaataatgt aaaatggatt gcaggtaaga ctgttaaagg tatagacata
    26101 aataaaaagg tagttcagac agaagacatt actgtaaatt atgataaact tttaatagct
    26161 acaggtgcat cttctgctat tcctcctata aaaaatttaa gagatggaaa ttttgtatat
    26221 tctgtgagaa acatagatga tatttataaa ataaaagaaa aagcacaaaa ttctaaaaat
    26281 gtagttataa taggtgctgg tttagttggt atagatgcat tagtgggatt atttaaatat
    26341 gagcagttaa atatttctgt agcttttatg gaaaagtaca ttttagatag acaattggat
    26401 gaatatacag catcagtgta tgaaaataag ttcaaagaaa aaggtgtaaa attttaccca
    26461 agtgcatcaa tacaagaaat tgtattggat aacagtaaaa atgttacagg tgttgcattt
    26521 tcaaatggtg aagttttaga tgcagacatg gtaattgtag ctacaggggt taaaccaaat
    26581 gctgactttt tagatggaac aggaatagaa tatgatagag gtataataat agatgatatg
    26641 tgccaaacaa cacaaaaaga catatatgca gcaggagatg ttgtaggtaa aaatgctatt
    26701 tggcctctgg cagtaaagca aggaattgta gctgcctata atatggttgg aaaagataag
    26761 aagatagaag atgaatttgc ttttaaaaac agtatgaatt tcatggatat accaactatt
    26821 tctataggaa tgaatacacc tgtagatgat agctataaag tattaaccag acatggaatt
    26881 aatgattata aaaagtttgt ttttaaagat aatgtaatct atggagcagt tatacaagga
    26941 gatatttcat atgtaggtgt attaacttat ttaataaaaa ataaagttga aatatatgat
    27001 ttagaaaata ggatatttga tgtatgttat gcagactttt ttaatataaa agaaaatgga
    27061 gaattttgtt atagtgtata gtatatacgt gaagtaaggt ttataaatac agtaaatcaa
    27121 taaatagagt tattctaaaa taagaaaaat gacaaatctt atttagaaat agctctattt
    27181 tttgcaatta aatttaaatt atattcaatt tgtaaaatat tattttgaac aattatacat
    27241 atactctcta gttagaggta aatcattttt tataccttta gtgaaaagta tttgatgtat
    27301 atctataata ccagtgttga aagcagcagc acaagaacaa aggtataaat cccacattct
    27361 tataaactta tcatcaaaca tttctttaat ctttggaaga ttctcagaga aatttttttg
    27421 ccagtatagt aaggttttta cataatgtaa tcttagactt tctacgtcta tagtatgata
    27481 tttgtaatca ctacaaatat gtatcatttc tcttaggcta ggaataactc ctcctgggaa
    27541 aatatatttt tttatccaag ggtctccata agattcctgc aatccactta tatagtgaag
    27601 taaaaacaat ccaccatctt ttaaaacagc atttacgttt ttaatgaaaa gttcatagtt
    27661 ttctctacca acatgttcaa gcatcccaac gcttacaact ctatcaaaca taaggtctga
    27721 tttttttaat tctctatagt ccataagtct tacctcaaga tagtcttgaa ggttctcttc
    27781 tttaattctt gtgttaaatt ccttaaattg ctcttcgcta agtgtaattc caacgccgtg
    27841 aattttatat ttttttgcag cttctattag taaaaatccc caaccacaac caatatcgag
    27901 aaggctcata ccttcctcta aatttagttt agttaatatg tggtgaactt tattgacttg
    27961 agcatcataa agagtatcat tatcatttct aaagtatgca caagaataac taagtgtctt
    28021 atctaaccat aagctataaa aatcgttgcc aatgtcatag tgggcatgta tttctttctt
    28081 ttgatgtctc attgaatttg atgagtgcaa tagatgtttt acagctgaat tatcaatatt
    28141 gaatttgtct atttgtttta aaagtagatt taaagttaag aataaatctc cctcaacttc
    28201 aatgtctcta tttatgtagg cttctccaag tgctaaagat gtacttttta atagttcaga
    28261 cttgctaata tcattattta ctataacttt gaactgaggg gagccctctc caattagaaa
    28321 ttcttcctca tctttaagcc taactagaaa tggtgtttca ccaaatctct ctaaatagtc
    28381 ttttaaaaaa ttttttgtaa tattcatatt accagctcct tctaatgtaa atccagtaag
    28441 tttccctgct taaaatatat tatagtcctt tttttaaaaa agtaaaattt tttcaataaa
    28501 ttttttattt aaaggaaatt tattttttat aaagaaattt catgttgtag tatatttttt
    28561 gcttataaat tttggtactg gatataatat taaactgtta atgatataat aaatttaatc
    28621 tataaagtaa ataaaatttt gtaataaata gttaaagcta ttttataagg agatatatga
    28681 tggaagtata tgctttggtt gggtcaagtg gcacagggaa gagctttaag gctttagagt
    28741 tcgcatatga aaatgatata gaatatataa tcgatgatgg gattttaata tacaaaaaca
    28801 aggttttagc tgggatatcg gcaaaacaag caaatactac tatagaagca gttaaaaggg
    28861 ctatttttta taatttggag catagacaag aagttagaga agcaattaaa aaagaaaata
    28921 taagaaaaat tcttattatt gggacatcaa aaaaaatggt taatcaaatt gtagagagat
    28981 tgtcaattgg aagggtttgc aagtttataa atatacaaga tataagtact aaatatgaaa
    29041 tagaaatagc aaaacaagct agaaaagaag gaaatcatgt aattcctgtt ccagcagttg
    29101 agataaaaag tatggcaagt ggtcttagta taaattcttt gaagcgactt tttagaaaag
    29161 gaaataatat gaatatggta gttttagaaa aaacaattat aagaccaact tttagttata
    29221 taggaaagtt ttatattagt gctgatgtaa ttaaacagat tattgaatat gaagtataca
    29281 actttggaag cattgataga ataaataaaa tcaatataga gaattgcaat aattttatga
    29341 atatttttgt aagtataaat atcaatgatt tagaaactat aaaaaaaatt gaagacatac
    29401 aacagacaat aaaaaagtct attgagaaaa tgactttagt taatgtacaa aaaatagata
    29461 ttaacattca taaaattaaa aaaataaact tcctccaatg ttaaaaaata aactgagaaa
    29521 atgatatact aatttgcaat aaagtgtgct ataattaaac tgtaaattta ggaaatggtt
    29581 ttttctaaat ggagtataca taattataac gtatttataa gggggctttc aaatgtcagg
    29641 aaaagatgta aatgtcttcg agatggcgca atctcaagta aaaaatgcat gtgataaatt
    29701 aggtatggaa ccagcagttt atgaattatt aaaagaacct atgagagtta tagaggtttc
    29761 aattccagta aaaatggatg atggttctat aaaaactttt aaaggattta gatcacaaca
    29821 taatgatgca gtagggccaa caaaaggtgg aataagattc catcaaaatg tttcaagaga
    29881 cgaagtaaaa gctttatcta tatggatgac tttcaagtgt tctgtaacag gtataccata
    29941 tggtggaggt aaaggtggaa taatagtaga tccatcaact ttatctcaag gtgaattaga
    30001 aagattaagt agaggataca tagatggaat atataaatta ataggtgaga aagttgacgt
    30061 tcctgcacca gacgtaaata ctaatggtca aataatgtct tggatggttg atgagtacaa
    30121 taaattaact ggacaaagtt ctataggtgt tataactggt aagccagttg aatttggtgg
    30181 ttctttagga agaacagctg ctactggatt tggtgttgct gttactgcaa gagaagctgc
    30241 tgctaaatta ggaatagata tgaaaaaagc taaaatagct gttcaaggta taggaaacgt
    30301 tggttcttat acagttctta actgtgaaaa acttggtggt actgttgtag ctatggctga
    30361 atggtgtaaa tcagaaggtt cttatgctat atacaatgaa aatggtttag atggtcaagc
    30421 tatgttagat tatatgaaag aacatggtaa cttattaaac ttcccaggag ctaagagaat
    30481 atctttagaa gagttctggg cttcagatgt tgatatagta ataccagctg cattagaaaa
    30541 ctctataact aaagaagttg ctgaatctat aaaagctaaa ttagtttgtg aggctgctaa
    30601 tggaccaact actccagagg ctgatgaagt atttgctgaa agaggaatag ttcttactcc
    30661 agatatatta actaatgctg gtggagttac agtttcttac ttcgagtggg tacaaaactt
    30721 atatggatac tactggtcag aagaagaagt agagcaaaaa gaagaaatag ctatggttaa
    30781 agcatttgaa tctatttgga aaatcaaaga agagtacaat gttacaatga gagaagctgc
    30841 ttacatgcat tcaattaaga aagttgctga agctatgaaa ttaagaggat ggtactaaga
    30901 tttactctta ataaataata ggatataatt atttaagata taaaggggct gtacatagta
    30961 cagcccttaa attatgtggc ttgcaatgaa gtaagctatt tttgttttaa cttataaata
    31021 acttgataaa ttatgtaatt tagaatgtat tttttaccac cacatgatta caaattagaa
    31081 gagttagatt agatttttag acatgaactt atgcatttta aaagcaaaga ccttaattta
    31141 aagtatctag tattattttt aaaaatagta tattggttta atccatttat atatataatg
    31201 gaaaaatata tggatttgga ttgtgaactt tattgtgatg agagagtttt aaaaaattgt
    31261 actgtacaac aaaagcaaga atatgctcta actattgtaa attcaatgaa aagaggtaca
    31321 aatttttcaa ataaatttgt agctggatta aataaacaat ctgatattag aaaaagagta
    31381 ataaacatgt tcagtgaaaa acataaaaat ggtatcttaa tggctttgat tataagcttg
    31441 ttttcttcta ttacattttt taaattcgat tcaactagta ttattgaaga gaatgatttc
    31501 ttagagaggt taagatatac gtttgaagga aggcaagaag atatgtttgt aacaatagat
    31561 tttacatatg cagatgcacc tataaaactt agaaaagagc atgaagaaaa ctgtagagtc
    31621 aatggaatag taccaaaaga ttcagattca atagaaatta ttcctagatt ttataaagat
    31681 tgtatggatt taaaaaaata agttagactt aatattgatg ttgatattat gaatttttta
    31741 tatttaagca attgaaaatt ttattataga ttcaatttta tatgtgttta agtatgaaat
    31801 ttatagatag ttgaaataat taaatatgat tgatgcatag gaatcattac taagctataa
    31861 ttaattatag gggagtttaa ataaattatg agttacacaa ttataaaatg ataaaaatag
    31921 ttatctgtta caaaaaatgt atctttttaa ttaataaaga taatatgaaa acttaaaagg
    31981 caaataaact taaatataaa atactcttaa ataagatagg agaaaaatgt atgtctaact
    32041 ttattctaaa tgggattttt agaacagtta tagtaagtag tctaagcatt cttctgatat
    32101 tgattttcaa gaaaaatgta tttaaaagat ttagcaaaaa atttaattat tatatatgga
    32161 tgatagttgt tattaaatta tttttaccat ttacatatta tacttttaca ataaatatct
    32221 tgagaagtaa aaaacacatt aatattaata acataaattt agagggattt aataatgttt
    32281 caactataag taataatatt atattgtaca cttggattat aactgtagta gtgtatttaa
    32341 tttacactat atttaaatat ataaaactta aaaatttaat aaatgactta tcttatgatg
    32401 ttgatgatag agagattgta aatctttata aaaatatatt agaagaattt aatataacaa
    32461 aagatataaa actcaaatac tcttatgaag ttgaaacacc agcatttttt aattcatgtg
    32521 tacttttgcc accacatgag tataaattga aagagttaga ctggattttt agacatgagc
    32581 ttatgcattt taagagtagg gacctttatt taaaatatgt aatcctgttt ttaaaaacag
    32641 tatattggtt taatccattt atatatataa tggataaaca tatagattta gattgtgaac
    32701 tttattgtga tgaaagagtt ttaaaaaatc gaaattcaga ggaaaaaaag gattatgctt
    32761 taacaattct aaatgcaatg agaaaaggtt caaatacttc aaataaattt atagctggac
    32821 tccataagca atctgatatt aaaaagagag ttatgaatat gtttaatgaa aagtacaaga
    32881 atggtatatt aatggcatta actttatgtt tattatcttc tattactttt ttgaaggtta
    32941 attcaacgag tattgttaat cttaatatat tgccaaaagg aatggaagaa aaagatgtta
    33001 ctaatagtgc aattgttaca atgatagaat ttacatatgc agaagctcct aaaagtatga
    33061 gagaagaaca taaaataaat tgtgaattct taggtttaat acctaaagac tctgataaaa
    33121 taggtgttgg tatagaatat tatgacagtc tattgaatga ttaaataaat attattttaa
    33181 cagatattaa ttataaagtt ttgaatagta atttgttgag aaataaatat atccttataa
    33241 aaatagatac ttatatacca agaataaaaa atagttaaga tagtacacaa ggatatactt
    33301 ataaagaact taaataagtg aaataaatta ttaattataa ggtttataat tttaaaaaat
    33361 tagataaatt caggtgtaaa atactataaa acacaattcg gaaaagtgta tgttgaactt
    33421 catttaaaat ggaatttcaa agttgctata gtaagtagtt taagcattct tcttatatta
    33481 atctttgaga ataatgtatt taaaaaagtt agtaaaaaat ttaactattg tatattaatg
    33541 atagttgcta ttgatatatt atctaaaaaa attaagtatg aaaagattac tgttaggaca
    33601 atcttttcat acttaatttt taatatttat attatccaaa attttcatct ttttaactta
    33661 gaatttgaga atcactatta ggaaataggc tttgatttat gtagggttaa aaatttcaca
    33721 gaatatctaa ctttatacta aatccatcaa aaaatatacg ccatataaaa gcgcataaaa
    33781 aaacacataa aacaatattt atgcgcactt taacctcgaa tggtacaaaa ttctttactt
    33841 ttacagaccc atattatata attaagtaaa taatgtatat ttaggtgaca tattggataa
    33901 gctaatttat ttattttatt aatataataa tactataaaa gttatataaa gtggtttaca
    33961 aaaaagttac agatagagct tttattaatg tattgtgtat tagtatttta tccttaggta
    34021 cacttcacct acaaaataga atcataagat aattatggga aaattttagg aggtttatcg
    34081 tgaagaaaaa gatattaata ccagtatttg catcagttat ggcattatca gtaagttcaa
    34141 ttgtaaatgc agatgaagta aatgattcat ctcaaaataa ggatgataaa acaaatactg
    34201 aattaaattt aggtgaatat aaagaagtaa aatataaagt tgcaaaaata aaagatggag
    34261 tagctataaa gataagagaa gaaggtcaag ttcaaaacat agcttattct ggagatgaat
    34321 ttacggtttt aggaactcaa ggagaatggg ttaaggttaa agttgaagat ggcgaaggtt
    34381 ggttagctac tagatatgta gatatatctg aaggtgtagg atatactaac gctgataaag
    34441 taaacttaag aaaagataag agtgaaagtt cagaagtaat tgaagagctg gaaaaaggaa
    34501 gttctttatt ggttttggaa gataatggag attggttaaa agttaaagat ggggaaacag
    34561 aaggatatgt aaaatcatct tatatatctg ataaagctcc tgtaatagaa gagccacaag
    34621 taaacccaag tgttaatcaa aatggagatt ctacaaataa taataatcaa caaaacaatg
    34681 caaataatat tcaacaagat aatacaaatg acaatcaaca aaacaataat tcaaatgtgc
    34741 caacagctaa ctctaatgca gttcaagctg tacttaactt ggcatattct aagcaaggtt
    34801 gtccatatgt ttggggagca gaaggaccaa acacttttga ctgttctgga tttacacaat
    34861 atgtgtatag aaatgcagta ggaaagaata taccaagaac atctaaagca caatctaaat
    34921 atggacaaac tgtaagtaaa gcaaatttac aaccaggaga cttagtattc ttcacaacta
    34981 atggttctgg ttctgttagt catgttggaa tatatgtagg tgggggtaat atgatacatt
    35041 caccatcaac aggaaaaaca gttagtgtaa ctagtataaa ttcatcatac tatacagcaa
    35101 gatttgttac agcaaaaaga atattataat cggtttgtaa tatgacaaat tgataaatac
    35161 ataaatatat ataaacgctc tatattatat ttaatttaat catggaaaca aaaaatgatt
    35221 atattttaaa tgtactatag agcgtttttg tatcacttta aatctaatca agattcaagt
    35281 taatatgact gttaaatagg agccttttca tatgatgatt atttgcgcaa tcccttgaca
    35341 ggatagggca aaggtggtaa gctactaaca gtacaaaaat acttttaatt ttagttcaga
    35401 gaggctaaaa aaggaggacg aaagatgtta aaatcaagaa atttaatcca accagaagac
    35461 ttttcaatag aagaaataga tgaaatatta gaattagctc aaaaaataat tgacaatccg
    35521 tcaaaatatt ctcgtatatg tgaaggaaaa ctattagcta cattatttta tgaaccatca
    35581 acaagaacaa gattaagctt tgaatctgct atgaacagat taggaggacg tgtagtaggc
    35641 ttttcagagc caaattcatc ctctgcatca aaaggtgaaa ctctaggaga tacaatgagg
    35701 atagtatctg gttatgttga tataatagct atgagacatc ctcaatcagg tgcagcttca
    35761 gaagcagcta gatatacgga agtgccattt ataaatgcag gagacggcaa gaaccagcat
    35821 ccaactcaaa cacttacaga cttacttact ataaaatcat taaaaggaac tttagaaagc
    35881 catacaatag gattatgtgg tgatttaaaa tatggaagaa ctgtgcattc tttggttaag
    35941 gctatggcaa gatataaaaa tacaaagttt gtatttatag cgcctgaaga gttaaaaatg
    36001 ccagattata taaaagaagc tataaaggga catgcatatt atgaaactaa taatttggat
    36061 gatgttatag gaagcttgga tgttttatat atgactagag ttcaacaaga aagatttgaa
    36121 gataaatcag agtatgaaag acttaaaaac tattatattt taaataaagc taaattagaa
    36181 aaggcatcta aagatatgtt agtaatgcat ccattaccaa gagtaaatga aatagacata
    36241 gatgtagata gtgatgatag agctgtatat tttaaacaag ctaaatatgg aatgtatgtt
    36301 agaatggctt taataataaa acttttaggt ataaatgaag actaatagtt atcatataat
    36361 agtaaaacaa aggagatttt gggatgtata aaattctgga aaatatatat ataggcgaag
    36421 atatgtatag aatgaaagta aaaggtaatt ttgaaggtaa aatgggacaa ttctatatgt
    36481 taagagcatg ggatacttat ccagtacttt caagacctat aagtatacat gatatagatg
    36541 aagaagggat aacttttctt tataaagtag taggagaagg aactcaaatt ttgagtaacc
    36601 taaaggtaaa tgatactata aaattggaag gcccttatgg taatggatat gctaaggttg
    36661 atggaaaggt agctctagta ggaggaggaa tcggtgtagc tccactatat ttggttgcta
    36721 aaaatataaa aaattgtgat gcatatcttg ggtttagaga agatgttata ttagaagatg
    36781 aatataaaca agtatgtaat aaagtctata caacagtagg aaatactttt gtaacagata
    36841 taattgatgt tgagaaatat gattatatat taacatgtgg tccaactcca atgatggaaa
    36901 aattagttaa gatggtagaa ggtactaaaa cgagaattat ggtatctctt gaaaaccata
    36961 tggcttgtgg agtaggtgcc tgtttagtat gtacgtgtaa aactaatgga ggaaataaaa
    37021 agacttgtaa agatggtcca gtgttctggg gagaggacgt gatttttaat gggtaattta
    37081 agtgtgaaat ttggaagtgt agaatttaaa aatccagtaa taatggcgtc tggaactttt
    37141 ggatttggaa aagaatataa tgagatttat gatatacaaa aacttggtgg tataagtagc
    37201 aagggtctta ctttaaataa aaaacctggt aacaatggta tgagagttca tgagacacca
    37261 tccggtatga tgaatagtgt tgggcttgaa aacccgggag ttcaaggatt tatagactat
    37321 gaattacctt tttttagtaa actagactta gtcagaatag ctaatgttgg aggtgggaca
    37381 ttagaagact acttattagg ggttcaaatg ttaaatgaca agcctataga cattattgaa
    37441 ttaaatatat cttgcccaaa tgtcaaagct ggtggaatgg cttttggtat aaaaaatgaa
    37501 gtggcaagag aagttgttcg tgaggtaaga aacataacga aactgccttt agtaataaaa
    37561 ttatcaccca atgcagaaga tattgtagga atggcaaaag tttgtgaaga agaaggtgct
    37621 gatggagttt cattagttaa tacttttaaa gcaatggcaa ttgatataaa aagtagaaga
    37681 ccagtatttg aaaatgtata tgctgggtta tctggaccag ctataaaacc tatagctttg
    37741 agaatggtac atgaagtgtg taaaaatgta aatatacctg taatgggaat gggcggaata
    37801 actaaagcca cagatgctat agagtttata atggcaggag ctacttgtat ccaagttgga
    37861 actgcaaatt ttatgaatcc tagaataggt attgaaataa tagatggaat taatgaattt
    37921 atggatagag aaggaataaa aagtttagac gaaataagag gaattatata aggtagaata
    37981 aatagtattt taaagataaa aaatatagat taaataaaca ataattttat aacattaaca
    38041 tggaggaaaa ataatatgag taaagtagat gtagtggata tattaaagaa aagtgatgca
    38101 ttgttggagg gacatttttt attatcttca ggaaaacaca gtaatagata cgtacaatgt
    38161 gcaaaagtat taagatttcc agaatatgct gctgaggtat taagtacagt tgttgaacaa
    38221 ataaaagact tagatataga cttagtagta ggaccagcta tgggtggagt aatagtttct
    38281 tatgagttag gaagacaatt aggaaaagaa gctgtattta ctgagagaaa agacaataca
    38341 atggagttaa gaagaggatt tgaagttaaa aaaggagcaa agataataat tgctgaagac
    38401 gttgtaacta ctggtaaatc aactatggag acaaaaagag tattagaagc cttaggtgga
    38461 gaagttgtag gtgttgcatg tatagcagat agaactaatc atgatatagg tatgcctata
    38521 tatagtgcta taaaacttga tattcaagtt tatgaatctg atgagtgtcc tttatgtaag
    38581 gaaggaaaat taccagttgt taagcctgga agtagggagt tcaaggaatt agggatgtaa
    38641 taaaaactta attatttata gtgttactta aaaaatgtaa attttttaga gtaaaaattt
    38701 gtaatataac atataactta aaaataaaaa gcagttagcc ttataaatga aggttagctg
    38761 ttttttttgt taaaaatata tttacaaaat aagagatagt agcagtacaa tataagtata
    38821 aggtaaaagt attatatatt agggaggggt aagtatgaaa aatcaattaa atgaaaatca
    38881 taaaaaggtt gtattttttg gagtaggtgc tgtaggagct acttttgcag aacaattttt
    38941 taactctaaa tatgatttta aaattctttg tgataatgaa agaaagaaaa gatatttaga
    39001 agaaggattt ataataaatg gaaaaaggta tgattttgat tatgtaacta aagatgagta
    39061 taaacaagag gctgatttta taattatagg tctaaaatat aataatttaa aagaaaatat
    39121 aaaagaatta gatggattag ttggaaaaaa tacagttata atgtctctgc taaatggagt
    39181 tgatagcgaa gagataatag gagaaagatt tggaattgaa aaaatggtat attcatatgt
    39241 taccaatata gatgcaaaga aaatcaataa taatattata cacactacta atgggataat
    39301 tgtatttggt aacaaagata atagtgaaga tagaaaaact aatataataa ccaaagtctt
    39361 tgatgatgta aatatagaat atactttatc aaaagatatt caacgagata tgtggtggaa
    39421 gtacatggtt aatattggtg taaatcaaac ttcagctata cttggtgcac cttatggagt
    39481 ttttcagagt tctgagcatt taagagaatt agcaaaatct gcaatgaggg aagttgttgc
    39541 tatagcacaa gcaaaagaca tatctcttac agaagatgat gtggaacatt cattacatag
    39601 aatactagaa cattcaaaag aaggaagaac atcaatgctt caagatgtgg aagctcatag
    39661 acttacagaa gtagatatgt tttctaagaa tatctgcaaa cttggaaaaa aatataacat
    39721 acctactcct ataaatcaga ctttctttta tatgataaaa gtaattgaaa gtagatttta
    39781 aagggtcttt gtaagaaata ttgataatta ttgattttaa attttattaa tgttatatac
    39841 tatttaagtt actaaatatt tttaactctt caatgaaaat agttgctgct tttgataaag
    39901 ttatatcttt tggaaatata tatgagaaat gtctgttgta gctgcttcca agctctatga
    39961 cagacagctc attgtttaga acaggtaagc ttgtcactag attagagact attgtaattc
    40021 ctaaattatt tctaacagat tctttgacag cataattact tccaaaaacc atcatacttt
    40081 ttggaataat ttctttaaca cttaaaaaca tatctaaata atctctggtt ccagaaccat
    40141 cctccctgac tatccatttt tggttttgaa gtttatcaaa actaaaatct tttaataaat
    40201 gactcttgta tggaagtgct aaaaccatct tatcttcaaa aaaatattct tgaataaatg
    40261 aaggggaaga gcaagtgcct tcaataagac caatgtctaa aatataatcc ttaacatgag
    40321 aagatacgat tgatgtattt tttataaaaa cttctacatc tatgtcagga tactttttag
    40381 aaaataatgc tagaaaattt ggtaaaatat actctccaat agttaaactt gcaccaatct
    40441 ttaagtgacc agttatagca tctgaaactt ggcttacatc atgataagta atatttaaaa
    40501 gatttaatat ttctttagct cttttgtaca agatttgtcc actttctgtt ataaaaatgg
    40561 ttttttgttt aatagacctg tttataagca caactttaaa ataattttct agatttttta
    40621 tgtgtttact tacacttggt tgtgatagat ttaaatattc cccagcttta gtaaagtttt
    40681 tgtattcaac aacagctaca aatgttttta attcttcaaa caattttttg tctcctttac
    40741 ctaatgaaat tttacatttt ttatatactt tctacagata attatactat atataataac
    40801 cattaaaaac attaatcatt acgatattat atatttattt tacttatggt tattacaatt
    40861 atatactatt gctatgaaga aaactatatt aattataagg taggtatatt atgttaaaga
    40921 aaaaaagcag tttgacatta aaaactataa gggaaatatt accaggactg tttgtatcag
    40981 tattagtagg ttatattagt atatttattt ctattttaat tcctaaggta ggggcagcat
    41041 caatttctat atttttaggt atgtttgtag gaaacctatt tttaaatcaa aaggtatttc
    41101 aaaaaggtta taaattttca gagacagatt tgttatcata ttcaatagta ctattaggag
    41161 caacactcag tgtatctacc ttaatagact taaaagtttc aggaatttta tttataatat
    41221 tgcaaatgac aataacaata atagctgcac tttatatagg taaaaaacta ggatttgaag
    41281 aaaactttag atttctaatg gcaagtggaa atgcagtatg tggttcttct gcaataggag
    41341 caacagttcc agtggttaat gcgacagata aagaaaaggg aattgcagta actattgtaa
    41401 atgttactgg tatatttctt atgtttttac ttccaataat atctcaattt ttatatagcc
    41461 atgaacttgt aaaaacttca gcaatgatag gtggaacttt gcaatcaatt ggtcaggttg
    41521 tagcaagtgg agcaattgtt ggagaaaatg taaaagattt agctactatt tttaagatag
    41581 taagagtaat atttctagtt gttgttgtat tagtatttgg acatatgaaa aatcaatcta
    41641 atagggaaat tcttgaagaa gaaaaggatg agataaaaaa gaaaaaagtg actataccat
    41701 ggtatgtaat tggattcttt ataacttgtg cacttttttc aatgaacatt ataccacaag
    41761 aagtatctgt gttgtgcaaa gaaataagta acaaactaga aataatagct ttagcagcaa
    41821 ttggattaaa ggttaatgta aaagatttag tgaaacaagg gaaagaagtt tctttatatg
    41881 gtttatttgt aggtacagta caagttgtat cagctgtagt tttaataaaa atatttatat
    41941 agcataaaaa tagtatttgt aatattaatt tgattaacta aaataatatg atactaataa
    42001 tttataatat aaataaaaaa tgaatttcga taagtttaag tgggtataaa cttatagaaa
    42061 ttcatttttt atttaaggta aaaatttaaa gtaaaatatt attttacata ataataacct
    42121 tataaatttt gaaattactt atctttgtgt taaaataagt aaaatgcaca tattagagct
    42181 aagtttttaa gtaaataaac aaaatgaaaa tataaatatt aaggagattt ggtatatgaa
    42241 tgtttatgaa aaaggtaatg gagttatact agaaggagta acagattttg acccagtaca
    42301 tatatttgag tgtggtcaat gttttagatg gcacaaacaa gaagatggct catacactgg
    42361 agttgcaaag gggagaatat taaatgtcaa aaaagagaat gataaaatat atttaaataa
    42421 tactaattta aaggagttta acagcatttg gtataattac tttgatttag gaactgatta
    42481 tgcagaaata aagaataagc taaaaaatat ggatgaatat ttaaataagg ctactgaatt
    42541 tggatgggga ataagaatat taagacaaga tggatgggaa atgcttatat catttataat
    42601 ttcctctaat aataggattc ctatgataca aagagcgata gaaaatttat caagaaagtt
    42661 tggtaagtat ataggtgaat atgaaggcaa tgaatactat gcattcccaa caccagaaga
    42721 actaaataag gcctcacaag aggaaataag agcatgtcaa acaggtttta gagataagta
    42781 cataaaaagt actacacagg cagttataga gaataatgat gaggtatctg aatatacaaa
    42841 tttaagtaca gaggattgta gaaaagagct tttaaaattt aatggagtag gaccaaaagt
    42901 atgtgattgt attgcattat ttggtatgca aaaatatgat tcttttccag ttgatgtttg
    42961 ggttaagaga gttatgcaag aattttatat agatgaagac atgagtttac ctaaaatgag
    43021 aacttatgga atcgataaat ttaaagaaat gtctggattt gctcaacagt acttatttta
    43081 ctatgcaaga gaactcggta taggaaaata aattaagggg gagaaattat gggagtaata
    43141 ggaacaatat ttctgttcta tttaattata tcatatctag ctggagccat aatttctgtg
    43201 attatattac ttgaaaatag agaccctgca aaaacaatgt cttggctatt gatgtttatt
    43261 atatttccag gtgtaggttt aatgatatat gctatttcag gtagaaatat taggaaaaga
    43321 aaattattta agactcaaaa actagctaat aatataaaag aaaagaaact atttgataca
    43381 ttagaaaaga taacagaaat agttgaatta gaaaaagaat ctataaaaca aaataagctt
    43441 ttaagagatg aagaggatgg tagttataga aaaagggtaa taaatatgtt gttaaagact
    43501 ggtatgtttc cctttactaa aaataataaa gtggatgtat ttgtagatgg aaatgaaaag
    43561 tttaaaagac ttatagaaga tataagagaa gctaaagacc atattcattt agagtacttt
    43621 atcatcaaag atagtgaaat aggtagggtg ttaaaagaag aacttataaa aaaggctaaa
    43681 gaaggcatta agataagaat tttatatgat gatgtaggat gctggagatt ttggtttaat
    43741 agaaaatttt tccgagaaat gagggaagta ggtatagaaa ttgcagcatt tttacctaca
    43801 aaatttccta taataggagg gaaattaaac tatagaaatc acagaaaaat tgttgttatt
    43861 gatggaataa ttggatatac aggtggaata aatataggag atgaatattt aggcaaaaat
    43921 gataagtttg gatattggag agatactcat attaggatta agggtatttc tgtatacatg
    43981 cttcaaatga catttctgat tgattggtat tatacgacaa aagaagtatt agtgactaaa
    44041 aattatttcc caagtgtagg gaatgttggt gaaagtatga tacaagttgt tgcgagtggt
    44101 ccagacagtg actgggaaga tattcattat gcttattttt ctgcaatatg tcaggctaga
    44161 aaaaatgttt atatagaaac tccatatttt attccagatg aaagtctact aaaagctata
    44221 aagagtgctg ctcttagtgg agttgatgtg agaataatat ttcctaaaat tgcagaccac
    44281 aagatagtaa atatagcatc ttattcatat tttgaagaaa tattaagagc aggtggtaaa
    44341 gtttatttat ataataaagg atttatacat tctaaagtcg taataataga tgataaaatt
    44401 gcttctgcag gtacagctaa tatggattta agaagtttta tgcttaactt tgaggtaaat
    44461 gcatttatct atgatgaaga agttattagg gtaatgacag atgatttctt tgaggattta
    44521 agtcactgtg aagaacttaa tttggaggta tttaagaata gaaatatcat acaaaaaatc
    44581 aaagaatctg tagcaagatt attttcacca atattataaa aaattattaa aaaaggtatt
    44641 gaaaattatg tgtgattggt atattataat aattgttagc actctattag atagagtgat
    44701 aataaataaa aagtatatat ataaatctac atatataagt agtattaatt taaaaataac
    44761 taaaaacata ggcgaactag tctatactta atattttagg aggcgtgaat aatgaaaata
    44821 agaccattag ctgacagagt agtaattaaa aaagtagaag cagaagaaaa aactgcaagt
    44881 ggaatagttt taccaggagc agctaaagag caacctcaaa tagctgaagt tgtagaagtt
    44941 ggaccaggtg gaatagttga agggaaagaa ataaaaatgg aattgacagt gggagataag
    45001 gttatattcc aaaaatattc tggaacagaa gttaagatag aaggacaaga atacacaata
    45061 ctaagacaga gtgatgtatt agctgttatt gaataaatat agaataaatt tattaggagg
    45121 ggtttaaaat ggctaaagaa attaaatttt cagaggaaac aagaagagct ttagaagctg
    45181 gtgtaaataa attagcagat acagtaaaag taacattagg acctaaagga agaaatgtta
    45241 tattagataa aaaatttggt tctccactta taactaatga tggagtaact atagcaaaag
    45301 agatagaact agaagataga tttgaaaata tgggtgctca attagttaaa gaagtagcaa
    45361 ctaaaactaa tgatgtagct ggagatggta ctacaactgc tacagtttta gcacaagcta
    45421 taataagaga aggtttaaag aatgtaacag caggggctaa cccaatactt ttaagaaaag
    45481 gaatacaaaa agcagtaaca gtagcagtag aagaattaaa aaatcaatca agaatagtag
    45541 aaacacaaga ggctatatct caagttgctt ctatatctgc tggtgatgaa gaagttggaa
    45601 aattaatagc agaagctatg gaaatagtag gtaaagatgg agttataact gttgaagagt
    45661 ctcaaactat gaatactgaa ttagatgctg ttgaaggtat gcagtttgat agaggatttg
    45721 tttctgcata tatggttaca gatgtagata aaatggaagc agttttaaac gacccatata
    45781 tattaattac tgataaaaaa atatctaaca tacaagaatt attaccagtt cttgaacaaa
    45841 tagttcaaca aggtaaaaag ttattgataa tagctgaaga tgtagaaggt gaagcattat
    45901 ctacattagt agtaaataaa ttaagaggaa catttgatgt agtagcagtt aaagctccag
    45961 gctttggaga tagaagaaaa gaaatgcttc aagatatagc aatacttaca ggtgctcaag
    46021 taatatcaga agaattaggt tatgatttaa aagaagctga tttatctatg ctaggtagag
    46081 cttcatctgt taaggtaact aaagaaagta ctacaatagt agatggttct ggagataaaa
    46141 aagctataga agatagagta actcaaatta aacatcaagt agagcaaact acttcagatt
    46201 ttgatagaga gaaattaatg gaaagattag ctaaacttgc tggaggagta gctgttgtaa
    46261 aagttggagc tgctacagaa gtagagttaa aagaaagaaa gttaagaata gaagatgctc
    46321 ttaatgcaac tagagcagct gtagaagaag gaatagtagc tggtggagga actgcttttg
    46381 ttagtgttat accagcaata ggaacactaa ttgagagttt agaaggagaa gttaaactag
    46441 gtgctcaaat agttaaaaaa gcgttagaag agccattaag acaaatagct ataaatgctg
    46501 gtcttgaagg tgctgtaatt gtgcaaaatg ttgttaattc tgaagcagaa actggatttg
    46561 acgcattgaa tgaaaaatat gtaaatatga tagaagctgg tatagttgac ccaactaaag
    46621 ttagtagaag tgctttacaa aatgctgcat caatagcaag tactttctta actactgaag
    46681 ctgctgttgc tgaccttcct gaaaaagaag atgcaggaat gccaggaatg ggtggcggaa
    46741 tgcctggtat gatgtaattt aaaaaattta aaatataaat tttaatataa agaactattt
    46801 cgacttagat tttaaactct aagttgaaat agttcttttg tatatatcta atttttgtat
    46861 atctataaat tcaatacaca tatttagata atcagtatct agtttgtaat aatattttag
    46921 agtagtattg ttatttttta ggttatgatt ttagatatat attaattaag gtataattgt
    46981 tgatagagaa taaatcaaat ttaaaaggat gcgataatat ggacttaaga aaattgcttg
    47041 aagaagttaa aaatgaaaat attgatatag attttgcgtt agaaaagtta aaagatttac
    47101 cttatgaaga ccttggatat gcaaatatag accatcatag agaattaaga aatgggtatc
    47161 cggaagttat ttattgtgaa ggtaagagtg atgagcatat tttagggatt attgataaaa
    47221 tgaatcaaaa aagttctaac atattgggga caaggtgcag aaaagaaact tttctgaaaa
    47281 ttaaagaaat ctataatcat gctgagtacg aagaggcgtc taaaattctt aagattcaaa
    47341 atcatgatat agagaatata ggaaaaggta aaattgtgat tgctacaggg ggaacttctg
    47401 atataccagt agcagacgaa gcttatcata cagctaagtt tttaggtaat gatgttgata
    47461 gaatatatga tgttggagtg gcaggtatac ataggctttt aaataaaaga cataaaatag
    47521 atagtgctag agtaatagta gccgttgcag gtatggaagg tgctcttgca agtgtaattg
    47581 gtggactggt tgatgttcca gtaatagctg ttccaacttc tgttggatat ggagcaaatt
    47641 ttggaggatt agcagcatta ctagctatgt taaatagctg tgcatctgga atatctgttg
    47701 taaatattga taatggattt ggagcaggat atttagcatc aataataaat aaactgtaaa
    47761 tacattgata taaattaaac aaaactatat aataaaattt aatttctatt atatttttac
    47821 tattaaaatt gaggagacat atatgagtga aaaaatactt tattttgata taataagcgg
    47881 tatatcaggg gatatgaccc ttgcaagttt attaaattta ggtgtgccta aggaaatttt
    47941 tttggaggaa attcataaac taagaatgag cgatgaattt aatataaaca tcagctcgaa
    48001 aactgaaaat ggaatagtag gtacaaaagt agaagtaatt acaaaagaaa aacatacaca
    48061 tagaaattta gtggatatat ttgaggttat agataaaagc actctaaacg aaaatgtaaa
    48121 atctaaagca aaaaaaatat ttatggtaat tggagaagca gaagcaaaag ttcatggaac
    48181 tacaatagac aagatacatt ttcatgaggt aggggctata gattcaattg tagatatagt
    48241 aggagcttct atattagttg acttactttg tgtagataaa gtttgtgcta gtacagtgtc
    48301 tgttggttca ggatttgtaa aatgtgaaca tggaattata cctatacctg cacctgcaac
    48361 tatagaaatt ttaaaaggtg tgccaattaa attaaataat gtaaatggag aatgtacaac
    48421 tccaacagga gctgcaatca taaaagtctt gtgtgatgag tttgtagatg agcttgaatt
    48481 ttctccaaag caaattggat atggtatagg tcataagaag tttgaagtac caaatatact
    48541 cagaactgta ttaggtgaaa aaaaaaagaa gaattaattt atgaaattag tgcaaacatt
    48601 gatgatatgt cttcagaaat ctattcttat ttgtatgaaa aaatattaga tgagaaagca
    48661 ttagatattt atacagagag catttatatg aagaagaata gaccagcaac aaaagtatca
    48721 attttatgta atgaatcaga tttagaaaaa tttattaaaa tattactttt agaaactagt
    48781 acttttggag tgagatataa tgcatataat agagaaattt tagatagaaa atttgtaaaa
    48841 ttagatacaa aatatggagt ggttacagta aaactaggat attataaagg aaaactagtc
    48901 aaggctactc cagaatataa tgaatgtaaa actatagcaa aaaatgaaga tattcctata
    48961 aaaaaagtgt ttgctgatat aaattttaga ataaaagaag aatttagtga aaacttattg
    49021 acataagtaa taaattttga taaattaata ttcagcctta ttaaataaaa gtaaacagaa
    49081 aaaaatatat aaaatataaa ctcactcgta tatgctcaga aatatggtct gagagtctct
    49141 accaagatac cgtaaatatc ttgactatga gtgaaattat tataccaaaa gacctagtta
    49201 agtaaatgtg taggctgagt taaactccct atgtgttggc tataagataa aggtattttg
    49261 gaagcctgta tagtaatttc acttttgcta gaaattatgg aagttactat acaggttttt
    49321 tgtgtaaaaa gatttaatat aatttagact gtattgtgtt aaaataaata tataaaaact
    49381 atattataca aggagagatg tgaatttatg aaacatgaat tagtacttgt aatagatttt
    49441 ggaggacaat ataatcagtt gatagctaga agagttagag aaaacaacgt atattgtgaa
    49501 atacttccat gtactgcaag catagaaaga ataaaagaaa aaaatcctaa agggataata
    49561 tttacaggcg gtccaaatag tgcgtattta gaagattctc caacaatatc aaaagaaata
    49621 tttgaattag gagtaccaat acttggaata tgctatggta ttcaaataat gtctcatgta
    49681 ttgggtggag ttgtaagaaa aggtaataaa caagaaaaag aatatggaaa aacagctata
    49741 acttatggaa aatcatcatt atttgaagga ataactacaa atagtgtatg gatgagtcat
    49801 acagatttaa tagaaaaagt tccagaagga tttactatag ttgcaaatac taatgattgt
    49861 ccagtagcag ctatggaaaa tgtagaacga aacctatatg gagttcaatt ccatccagag
    49921 gtagaacact gtttggaagg ggacaagata cttacaaact tcttatataa tatttgtaaa
    49981 gtaaaaggtg actggactac agattcattc atagaagata aaataaaaga attaaaagaa
    50041 aaaataggcg ataaaaaagc tttatgtgct ttaagtggag gagttgattc ttctgttgct
    50101 gcagttctta ttcataaagc aattggggac aacttaactt gtatatttgt tgaccatggt
    50161 ttacttagaa aaaatgaagg aaatgatgta gaaagaatat ttagagagaa atttgatata
    50221 aatttaataa gagttaatgc agaagataga ttcttatcta aattaaaagg agtttctgaa
    50281 ccagaagcta agagaaaaat aataggtgaa gaatttataa gagtatttga agaagaatca
    50341 aataaattag gaaaaatgga tttcttagta caaggaacaa tctatccaga tgttatagaa
    50401 agtggacatg gaaatgctgc tactataaag tctcatcata atgttggtgg aatcccagaa
    50461 gatgtagact tccaagaaat tgtagaacca ttaagagagt tatttaagga tgaagttaga
    50521 aaaataggat tagagcttgg tatagaagaa ggattaatat ttagacatcc tttcccagga
    50581 ccaggtctag gaataagagt tataggtgat gtaactaaag aaaaatgtga tatattaaga
    50641 gaagctgatg ctgtttatat ggacgagctt agaaaagcag gtttatacag agaaatatgg
    50701 caagcttttg caacattacc agatgttaag actgttggag ttatgggtga tgaaagaaca
    50761 tatgcttatt tagttggttt aagagcagtt acttcttctg atggaatgac ttctgactgg
    50821 tataagatgc cttatgatgt attagaaaga atttctaata gaataataaa tgaagttgat
    50881 ggagtaaata gagtagttta tgatataact tctaagcctc ctggaactat tgaatgggaa
    50941 tagtaaataa aaaagtcgta agccttgtat tttcaaggtt tgcgactttt aaaaaaatct
    51001 ttgtgatact aaaatgatac tatatattta aattatctag tttttgggca acttcaagtt
    51061 gcttatttgg gtataaatgt gaatatgtat tccaagttgt ttctactttt tcatgcccca
    51121 atctttctgc tatagttaat atatttacat ccatatttac caataatgac gcatgagaat
    51181 gtctcaaatc atgcactcta attcttttta cattagataa cttacagcat ctatctagct
    51241 ctttagaaag ataactttta gcaaatttaa atattctttc attatctttt aagtcatata
    51301 gcttagatag ataatctttt atattattat ataagaaatg tgggataggg acaacacgtt
    51361 tacttttagg agttttagga gaagaaacaa tatcctctcc atttagtctt atgtagcttt
    51421 tttcgacgct tattttattt tcgaaaatat cttttggagt aagagctaac aactccccta
    51481 gtctaagacc tgtccaaaat aaaatttcaa atgctagtct aggttctgat ttcttttcaa
    51541 attcaataaa ttttttaaac tcttctaatg tccaaaagtt catttcatct gcgttttttt
    51601 taccgataga acctgccaaa tgagcaggat tagaaggtaa gttataatat ttaactgcat
    51661 aatttaatac agcaacaagc tggttgttta tcgttttaat atatgtttgg ctataatcac
    51721 tttttaataa ttcgttttgc catcttctta tatgtgttgc agtaatttca tttattttta
    51781 gacttttaaa aaaaggtaaa attctaaagt ttattaaata ctttttagtt tctaatgtag
    51841 acaattttaa tctagaagac atatcgtgca tgtattcttc tatgagactt tcaaaactca
    51901 tatcagtact cattttagat ttattcaaaa attctctttc aaactctaga gcttcttttt
    51961 tagttttaaa acctctctta attttctttt tcctatcccc atcgaagtct gtaaaataaa
    52021 agctagcata ccaacttttt ctttgttcgt ctttgtaagc aggcatttta gacaccccca
    52081 attatgtcat tttaatatta caataattat aacatgttag atatgctatc taaataattg
    52141 ttttattttc ctcttctttt tatttcttga agcttgtaat atttacacag ttctaagaag
    52201 tcttcttcta gtgctataga taagtctaat atcgtagata tacctatatc tttgtactct
    52261 tgattttcta gttttgatat ataacttctg ttacgtctca ttttttctgc taattctagt
    52321 tgtgtcattc ttttcttttt tcttaatttt ttcaacattt attaaaatcc accttttaat
    52381 tatttttatg acatttttat aattaatagt ttgtgtaatt tcttgtaaaa aatgttcctc
    52441 attagaacat tttttggtgg aaaaatgtgt taaaatgtag ttaagaaatt aaaagcaaaa
    52501 agcactataa caatagtgct aaaatggtag agtttaaaga aaaaataaat tttatttaaa
    52561 agcttataaa tataaagtaa ataatttgta aattaaatct aagaataatg taaaaaatgc
    52621 aataaaaaaa taaaaattaa aattatgaaa aataaagcta gtcgactaac taaaaataga
    52681 aatatacaga taatctaaaa tactagaaat aaaa
//
LOCUS       DAMJYC010000021        51568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJYC010000021 DAMJYC010000000
VERSION     DAMJYC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.022"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            35..373
                     /locus_tag="PC472_001292"
     CDS             35..373
                     /locus_tag="PC472_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2726626.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="PC472_001293"
     CDS             complement(500..1789)
                     /locus_tag="PC472_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2726627.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="PC472_001294"
     CDS             1869..2573
                     /locus_tag="PC472_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2726628.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="PC472_001295"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="PC472_001295"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2726629.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3729..5141
                     /locus_tag="PC472_001296"
     CDS             3729..5141
                     /locus_tag="PC472_001296"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2726630.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="PC472_001297"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="PC472_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2726631.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="PC472_001298"
     CDS             7243..9981
                     /locus_tag="PC472_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2726632.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC472_001299"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="PC472_001299"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2726633.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="PC472_001300"
     CDS             11217..12578
                     /locus_tag="PC472_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2726634.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="PC472_001301"
     CDS             12666..14555
                     /locus_tag="PC472_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2726635.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="PC472_001302"
     CDS             14570..15025
                     /locus_tag="PC472_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726636.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15084..15401
                     /locus_tag="PC472_001303"
     CDS             15084..15401
                     /locus_tag="PC472_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2726637.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="PC472_001304"
     CDS             15426..16688
                     /locus_tag="PC472_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2726638.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="PC472_001305"
     CDS             16793..17857
                     /locus_tag="PC472_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2726639.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARNIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="PC472_001306"
     CDS             17911..19089
                     /locus_tag="PC472_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2726640.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19280..20338)
                     /locus_tag="PC472_001307"
     CDS             complement(19280..20338)
                     /locus_tag="PC472_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2726641.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20405..20776
                     /locus_tag="PC472_001308"
     CDS             20405..20776
                     /locus_tag="PC472_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2726642.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20813..21487
                     /locus_tag="PC472_001309"
     CDS             20813..21487
                     /locus_tag="PC472_001309"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2726643.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21736..22575
                     /locus_tag="PC472_001310"
     CDS             21736..22575
                     /locus_tag="PC472_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2726644.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22819..22915
                     /locus_tag="PC472_001311"
     tRNA            22819..22915
                     /locus_tag="PC472_001311"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22853..22855,aa:Sec,seq:tca)
     gene            23066..23773
                     /locus_tag="PC472_001312"
     CDS             23066..23773
                     /locus_tag="PC472_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2726645.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23915..24751)
                     /locus_tag="PC472_001313"
     CDS             complement(23915..24751)
                     /locus_tag="PC472_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2726646.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            24939..25844
                     /locus_tag="PC472_001314"
     CDS             24939..25844
                     /locus_tag="PC472_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2726647.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25853..27037
                     /locus_tag="PC472_001315"
     CDS             25853..27037
                     /locus_tag="PC472_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726648.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            27152..28453
                     /locus_tag="PC472_001316"
     CDS             27152..28453
                     /locus_tag="PC472_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2726649.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYAFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            28407..29450
                     /gene="holA"
                     /locus_tag="PC472_001317"
     CDS             28407..29450
                     /gene="holA"
                     /locus_tag="PC472_001317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2726650.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(29637..29903)
                     /gene="rpsT"
                     /locus_tag="PC472_001318"
     CDS             complement(29637..29903)
                     /gene="rpsT"
                     /locus_tag="PC472_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2726651.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            30502..31023
                     /locus_tag="PC472_001319"
     CDS             30502..31023
                     /locus_tag="PC472_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726652.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            31057..31833
                     /locus_tag="PC472_001320"
     CDS             31057..31833
                     /locus_tag="PC472_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2726653.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            32175..33128
                     /locus_tag="PC472_001321"
     CDS             32175..33128
                     /locus_tag="PC472_001321"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2726654.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            33146..34165
                     /locus_tag="PC472_001322"
     CDS             33146..34165
                     /locus_tag="PC472_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2726655.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            34196..34447
                     /locus_tag="PC472_001323"
     CDS             34196..34447
                     /locus_tag="PC472_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726656.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            34729..36534
                     /gene="lepA"
                     /locus_tag="PC472_001324"
     CDS             34729..36534
                     /gene="lepA"
                     /locus_tag="PC472_001324"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2726657.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            36683..38020
                     /locus_tag="PC472_001325"
     CDS             36683..38020
                     /locus_tag="PC472_001325"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2726658.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIHCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            38253..39566
                     /locus_tag="PC472_001326"
     CDS             38253..39566
                     /locus_tag="PC472_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2726659.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            39738..40913
                     /locus_tag="PC472_001327"
     CDS             39738..40913
                     /locus_tag="PC472_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2726660.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            41016..42035
                     /gene="hrcA"
                     /locus_tag="PC472_001328"
     CDS             41016..42035
                     /gene="hrcA"
                     /locus_tag="PC472_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2726661.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            42065..42685
                     /gene="grpE"
                     /locus_tag="PC472_001329"
     CDS             42065..42685
                     /gene="grpE"
                     /locus_tag="PC472_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2726662.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            42777..44624
                     /gene="dnaK"
                     /locus_tag="PC472_001330"
     CDS             42777..44624
                     /gene="dnaK"
                     /locus_tag="PC472_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2726663.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            44835..45989
                     /gene="dnaJ"
                     /locus_tag="PC472_001331"
     CDS             44835..45989
                     /gene="dnaJ"
                     /locus_tag="PC472_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2726664.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            46955..47890
                     /locus_tag="PC472_001332"
     CDS             46955..47890
                     /locus_tag="PC472_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2726665.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            47926..49299
                     /locus_tag="PC472_001333"
     CDS             47926..49299
                     /locus_tag="PC472_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2726666.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(49487..50254)
                     /locus_tag="PC472_001334"
     CDS             complement(49487..50254)
                     /locus_tag="PC472_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726667.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEMLGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(50284..51393)
                     /gene="ugpC"
                     /locus_tag="PC472_001335"
     CDS             complement(50284..51393)
                     /gene="ugpC"
                     /locus_tag="PC472_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2726668.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT        20388 a         5521 c         8803 g        16856 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga aaaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatagtata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaacattat tttcagtcaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatg aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacag taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aactaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgacgataga agagttggat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttca gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagatg acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggaact tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccgcaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aggtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacgttagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgatttg gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtgta attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat cttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagaggat attatcacaa ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaacta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagag
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatattatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt gccacttgga acatagaaaa
    10561 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca ttttttttag
    10741 catattcatc atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatata aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagacatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tggaatttta tcaatggtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtactgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtgggtgt ttatccagtt agatatctcg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac agttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gaggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca cttataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
    15001 aataaatgaa gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca ttcataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggacta ggattaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat cttttgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat
    17401 agagtatgga gattttgtta cttttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag ggttttaagg ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagaa acataattgg ttcatatgga tatgccaata attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttataccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaat acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagagta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtaaatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctactga catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataaggatat aagggacaaa tttagaaata aattaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtattag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat
    19141 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    19201 aaatttgcat tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag
    19261 atataattat tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg
    19321 taagtttaaa attatctttt atacccttag ttaagtatac tttcttatcc ttagttatga
    19381 atatagcatc tacattatca attttattaa ttaaatccat acctttttca agaccaagtc
    19441 caaatgtaga agtggataag gcatcacaat taatagattt atcagagatt attgtaacac
    19501 tacttaaatt gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt
    19561 ttccgttttt ttcgataaat ctttcataaa ttccagaagt aacaacagac ttatttgtag
    19621 tttctatgtt tcctatggat tcaccatttt tactagttgg gtcttggata cctattttaa
    19681 aaggtttatc atttttacca ttttctaaag taaatatatt tcctcctaag tttactaaac
    19741 ctttttttac gtcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag
    19801 ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt
    19861 catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac
    19921 ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta
    19981 tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg
    20041 tatcatcaga aactctgaca aattttttac cagcattatc atttatttta gaaacatcac
    20101 ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat
    20161 ttaagatttt atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac
    20221 caaggtagta gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa
    20281 ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg
    20341 tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca
    20401 aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg
    20461 ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga
    20521 taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagaatag
    20581 aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg
    20641 ccctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg
    20701 tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga
    20761 tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa
    20821 cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa
    20881 cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa
    20941 tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca
    21001 agacccttat catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt
    21061 gaaaactcca ccatctttat taaatatgta taaagaatta aaagatgatt taggttgttt
    21121 tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac
    21181 tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt
    21241 cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg
    21301 gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga
    21361 agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc
    21421 taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa
    21481 tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg
    21541 taactaatag aaatatgctt aaatagttga aaatgctatg tatgcatatt tctatttttt
    21601 tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata
    21661 aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatctta
    21721 gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca
    21781 gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta
    21841 gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta
    21901 gaatctcaaa acattagcta tgatgcaaat gatatagtaa ctttagatga aaatacaaag
    21961 ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact
    22021 aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct
    22081 aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac
    22141 aatggaaaga aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat
    22201 aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt
    22261 tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg
    22321 agagtggtag caactgcata tactggtcac agtataactg caacaggaac aaaacctaag
    22381 tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct
    22441 caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt
    22501 gatatctaca tgaatgattc aaaaacagtt tataactggg gaagaaaaac tatagatata
    22561 cgtatattaa aatagttaaa taatttatat ttttactgga aattatatct tgattatata
    22621 gagaagaaac tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt
    22681 aataaaacag gtaaatttta accatgtata gataaaagct atttaaatta taatttacta
    22741 tcatataaat ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt
    22801 tgagaaggat ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact
    22861 agtatgaggg gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg
    22921 taaaaggtat cttttaatta aaagatacct ttttaaattt attttaagat aatttatttt
    22981 taataatata taatgttttg tattatataa tgtaaatttt ctaatactat tatatattta
    23041 gataaaaatc taggagtgtg gaaagatgga aaataatttt acaacgagaa ctagttttct
    23101 agttggagat gatgggataa aaaaacttaa taattcaaat ataatagtat ttggagttgg
    23161 aggagttggt agttttactg ttgaagcttt agcaagagct ggagtaggca atattactat
    23221 tgtagatttt gatgatgtag atataaccaa tataaataga caaataccag ccctacattc
    23281 aactgtagga agatacaaag ttgatgtcat ggaagaaaga atattagata taaatcctaa
    23341 tatcaatata aaaaaaataa gaagtcttta taataaagat actagtgacg aaatactaac
    23401 tgaaagatat gattatgttg tggatgcgat agacatggta agttccaaaa tacatctaat
    23461 tgaaacttgt gaaaaaaaag gattgaaaat aataagttct atgggtatgg gaaataaatt
    23521 agacccaact aaaatagtcg ttacagatat acacaaaaca agtacttgtc ctttagctaa
    23581 agttatgagg aaagaactta gggatagagg tatcaaaaag ttaaaagtag tatattctac
    23641 tgaacaacca atagaactta agaagaaagt aatgaatggt agaaaagtaa cacctggtag
    23701 tgtatcattt gtcccatctg taggaggttt aataatcgca tctgtaatag taaatgaact
    23761 attaggacaa taagttttaa atattttaat ttataaattt tatatacaaa tttgaaggct
    23821 agaagcatga atataaagca attttatatt catactttta gcctaattat tttatattac
    23881 cttattttat gtagttgtag ttttattttt tagattatgt atctatttca taattatatt
    23941 ttgtaaatat aatttctcca tcactataaa aaacaatatc tcctattcta tctgttctat
    24001 aaattgatac atggttttgt tttagattat taataacctc cctatgtgga tgaccatatt
    24061 gatttttata tccacatgat attgcagcta tatctggact aatttttttt ataaattcta
    24121 aagatgaaga agaattactt ccatgatgac ctatttttat aaaattaata ttatttaatt
    24181 taaaagaatg taaaatttct ttttcatttt cttcttcagc atcacccata aataaaaaag
    24241 aattatcatt aaaagttaat ttgaaaacta tagaatttaa attagattct tcttgtatat
    24301 atgatggact taatacatat atatttatat tgttatctat gtttaaaaca tcatttttat
    24361 agagatgctg aatagataag tttttatccg tacaagaatt tattagattt tgataagcct
    24421 cactatcagt tgattgttca ggcatatata tggagtttac attaaatttt tttataatat
    24481 tatcaagact tcctatgtgg tctgaatctg gatgagttgc tattataata tcaatagttt
    24541 ttattctttt ttgtctaaga taactaataa ttatattttc gctatcttca tcacctccat
    24601 caattaatat attcttattt gtgggtgttt gaactaaaat actatcacct tgtccaacat
    24661 caatcatatg tatagatagt agagattttt tgtcacagcc gattaaaagt gatattataa
    24721 taagtattga taaaaaaatt ttatttctca ataaaaaatc actccattca ttacatgttt
    24781 atataaattt atgatgggta atataagata tatatgactt attatatttt atattttaaa
    24841 caatataaat gaaattaata ccataataaa attggagttt tttatatata caaaataaaa
    24901 tttttaaaat taagcaataa aaaaggtagg aggattttat gtttttcaaa gatattgaac
    24961 taaattcaaa aaaagagtta gacccatatt ttgatttagt agattatgaa gcttgtgagt
    25021 actgttttag tactttatat atgtggcaac atgtttataa gacaggatat tatataggtg
    25081 aagattttgc cgttttagtt ggggaatacg aaggagatag cttctcaata ttaccacttg
    25141 ctaaaaaaga taaacttcca gaagttgtag attttgtcct tgaatatttt tctaaaaata
    25201 ataaaaaaat atacttaaga ggtattacta ctgaagtagt agagttttta aaagaaaaat
    25261 atccaggtag atttgagtat atagaagaaa gagatttatt tgactatatt tatgatgcag
    25321 aaagtttaag aactctagct ggtaaaaaga atcaaaagaa gagaaaccat ataaactatt
    25381 tcttaaaaga gtatgcaggt agatatgaag ctaaattatt agataaagaa aactttgatg
    25441 agtgtttagt actaatgaag gaatgggaaa gtaataagga agaaaataat gaatttgatg
    25501 aaagtatgga tgatgaatta ataggtatca aaaagatatt taaccattat gacatattaa
    25561 aagataaggt aaaagtgttt ggtgtttatg tagatggaaa attagaagct tttagtatag
    25621 gagaactttt aaatccaaat atggcattga ttcatataga aaaagctaat cccgatataa
    25681 gaggtctgta tccatttata aatcaacaat ttttggtaag tgaatttaag gacgttgagt
    25741 ttgtaaatag agaagaagat ttaggtattg aaggattaag aaaagctaaa ctttcttatc
    25801 atccttgcag atttgttgaa aagtacagtg ttagggaggc ttaatagact atatggagat
    25861 taggtatgct aaagaagaag agatagaaag cataaaagag atttggagtt attgttttaa
    25921 cgatacggag agttttatga agtactattt taatgataaa tataaatctg aaaatactgt
    25981 agtagcttta gatgaaggaa aaattatttc ttctcttcag ctaaatcagt ataagttatt
    26041 actaaatagc aaggtatata atacatcata tgtagtagga gtttctacac ttccagaggg
    26101 aagaggagct ggatatatga acaaagtaat gaaatttact ttaaacgaat tgtataaaaa
    26161 agggcaatta gtttctatat tgatgcctat tgactataga ctttatagaa gatttggata
    26221 tgagcattgt tacgaccaga ttgaatatac tattaacaca gatgatttaa aaaattttaa
    26281 gtcaagtgga aaaatgataa agtctaattt atcacaaatt gatgatttga tacaaataga
    26341 tagaactttt ttaaatgagg ttaatggtaa tgtgttaaaa gatgaacatt attatgaaaa
    26401 cttatttaaa gagattcaaa gtgaagatgg atttttatac attcatgaag gaaatgaaaa
    26461 agatggatat atagtttatt ttcttcagga agataaaatg tttgtcagag agttatttta
    26521 taaaaatata gatgccctta aatcaatgct taaatttata tataatcaca atactcaatg
    26581 taagatagtt actatatcta ctcctacaat agataagata aggtttatat tagacaaccc
    26641 taaagatagt gatataaaaa taaaaccttt tatgatggga agagttataa atgtaaagaa
    26701 gtttattgaa gatatagata ttgagaatga tattaatagc tcatttaatt tattaataga
    26761 agacaagttt atagatgaaa ataatggtct atttaaaatt tctatacaga ataagaaggt
    26821 atctgtagag cagttagata aaaaaggtgc agaaaagcct caagaagatt ttgatattaa
    26881 attagatata aataccttaa cacagttatc attttcttat atagatgtaa atgaagctat
    26941 tttcttaaat gatataaaag atgtttcaga agaaacacta gaaacactta attgtatttt
    27001 ttctaaaaaa aatacctata taaatgaata tatataggta gagtacgtta aaagatttta
    27061 aaaatctttt gacgtatttt tttgtttaga taacaattta tcttgtttaa aaattttcta
    27121 tttgaaaata atctaaataa tatactttta tatgggagtg attataattc aaataagaag
    27181 accattatta attatattaa tgtttgtggt agctatatca tttatcatta caaataacac
    27241 aaaaaacgac gaagaactta atgataagat aataactata caaggagttg tcaaaggaaa
    27301 aattgaaaag aaaagataca atcagtacaa ggtaggggtt tttctaataa atgattatac
    27361 aaaaaataaa aatttgaaga ttggtcagaa agttaatatt acaggtaaat tcaaaagttt
    27421 agataaaatg aagtatgatg attttgatta tggaaggtat ataaaaagta ctggatataa
    27481 aggtatagtg tatataaata actataaaat tattgataaa aataaacttt actctttaat
    27541 aggggaaata aagttttata ttagtaaaac ttataggtac ttatataaga aaaactcaga
    27601 ttttataaat tctatactat tagcagaagt tgaaaatttg actgaggaac aaaaagaaat
    27661 ttttactaga actggaacta gtcatgtaat atctatatca ggtcttcata caggaatatt
    27721 atgtgtaatt atatcctttc tattaagagg tataaataaa ttgtataaat tattaatact
    27781 gtcgattttt ataactttat attgtataat ggtaggtgcg tctccatcca taattaggtc
    27841 tatagcattt gttatgattt tttatttatc aatatttatt gatagaaaga aagatggaat
    27901 atcggcattg tctctaattg ggataatttt aattatgaat aacccctatg ttatatataa
    27961 tgtaagtttt caattgtctt ttttagctac actttctata ttaatattct ataataaaat
    28021 aaatagtatt ataaaattaa gtatggtatc attgacaata tcatcaaata tattaacttt
    28081 acctataatt tattatgcat ttaaaggtat accattgctt tcaattatag gaaatttaat
    28141 tatagtacca tttgtgggag taataatgta tttaagtatt gcaagtttaa tcgtatttaa
    28201 agtaagtgta gtaatagcta aaattatatc tttttttaat agtacattga tagaaagcat
    28261 atttttttta ctagaaaaaa tcagcaattt aagttttgca tacattaata tagaaagtcc
    28321 taaattttat attgttgtta tttattacat aggagtgttt ttttacatat tttacataga
    28381 aggaaaagag ataaaggagc aagagaatga attacaagga tattataagg aatgtaaaag
    28441 agaaaaactt tgaaaaaatg tatctttttt atggaagaga gtattattta attgagaatg
    28501 caataaaagc atttaaagat agtctaaacg agggaatgct tgattttaat ttagatataa
    28561 tagatgggaa ggaaatagtc ttaaatcagc ttataagctc tatagaaaca cttccattta
    28621 tggatgatag gaagatagta ataataaaag actttgaact attaaaagga aagaaaaaga
    28681 attttacaga tagtgatgag aaatacttaa tagagcattt agacaatatt ccagacacca
    28741 caactatagt ttttgttgta tatggagatg tagacaaaag aaaatcatta gttaagaaaa
    28801 taggaaacaa tggaattgtt tttgattgtg ataagctttc tgatatggat ttgtttaaat
    28861 ggataaaaaa atcttttgct ttaaatgatg ttataataga taattctcaa atcatgtatt
    28921 ttattgaaca agaaggatac agagataaga gtagtgaaaa gacattatct gatttagaaa
    28981 atgaaataaa taaaataagt tcatttgttg gtaaagggaa taatgtaacc aatgatgtaa
    29041 ttgataaatt atctcaaaaa aaagttgaga atgatatatt caaattaatc gattatattg
    29101 gagaacaaaa tgcttctaat gcaatgaaaa tattaaatga tatgatacaa gaaggcgaat
    29161 ctgtattagg tatattttct atgatagcaa gacaatttaa aataattatg caagtaagac
    29221 agttacaact tgatggatat tcaacaaaac ttatagctga taaattaaaa atgcatcaat
    29281 ttgttgtagg aaaagcatta aagcaaacta aaaatttttc tgatgatata attgtggaaa
    29341 tattaaacta tatattagaa agtgattaca aaataaagac aggtcttata agagatactt
    29401 tagcagttga aatgttggtt agtagatact gtaaaagaga agcaatttaa aaaatctata
    29461 tattaataaa aaataaatag caagatatgt tatagttcag agtagatgta agctaaatta
    29521 agtttgatat acaatcaact ataatatgtc taactattta ttttttattg tataaattta
    29581 caaaataaaa aacccctaca taggggttat tatatataag aaatataaac tggtaattaa
    29641 gcgttcatac cgtttaattt ttgagctaat ttagctactt ttctagctgc agcattctta
    29701 tgtatagttc ctttagaagc tacttggtat attctttttt gagcgtattg gaacttagca
    29761 acagcatcct ctctgtttcc agctgcaaca gcatcttcga atcttcttat tgcagttttt
    29821 atttgagatt ttctaactct atttaaagca gttttctttt cgataacact tattcttttc
    29881 ttagctgatt ttatatttgc caccgtgttc acctccctgt atttttttat catattcatc
    29941 gtcaacatat tagattttaa cagaaataaa tctaaaaatc aaggattatt atttatatat
    30001 tttaataatg ttttttattt tgatttttta gactgtaaat tgtgtataaa taatttgcaa
    30061 tatcttgttt ttaaattggt atattttgtg ttttaaatat tttgacatat ataaattaat
    30121 attttatttt tgtaacaaat attttgagat aatacaaact attaacatac attttgatag
    30181 tatttccaat aattataaga taatacaaga aattactcat ttttataaat ttaatgaaaa
    30241 aatataaaaa tataaattta tatgcttaat taagataatt tttccaataa tcaagataat
    30301 ttttttcatt agtatgtatg atagatgcat caaacaatat aactaatatg ttggaaggtt
    30361 atatgtaatt aatctcagtg tttatataga atagcatttg tagcatttat tatagttgta
    30421 attactattt atatcatagc cttgaatagt ctaaaaaaat agactataat atttttatag
    30481 actaaagaaa gggctgataa gatggacata gcaaaactaa ttttaaatcc tgcaaggctt
    30541 cgtattttac aatatattcg acttcatagc agtgttcgca catctgatat tgtaaagtat
    30601 ttaaatgata ttcctcgtgc aacagtttat caccatgtaa aaattttaga agagaacaat
    30661 atgattgaag taattaaaga aaatcgtgtg cgtggtacta ttgaaaaggt ttatgcacta
    30721 aaggaatata caacatctat ggaaggagag accttcgttg cactttctac agcattttat
    30781 gtaggtctga tgcaggaaat gaatgaatac tttagcagaa aagagcaaga ccataaaaag
    30841 gataatgtat ttttctcttc agcattacta tatatgagtg ataatgaata tgaaaatctt
    30901 cttaaaagta tagctgattt attgaaacca tatatagaac aaaaacctaa atcaaatcta
    30961 aaacttagaa aactttcaat tatatcttct ccacctgtag ctgataatga taaaacaaaa
    31021 taacatgcaa cccattttta taaaaggagg ttatatatga aatttcatga gtttggaaat
    31081 aaaaacagac cacatattat gttgattcat gggggaggga atgcatggtg gaactatttg
    31141 cgacaagcag aagttttgtc taacaaatat catgtgatac ttcctacatt ggatggtcat
    31201 ggagaagaat atgaaacaac atacatttct acagaggata cagcagataa gttgatatca
    31261 tatatagatg aaaattgcag tggacatctt ttcgctttgt gtggggtatc acttggtggt
    31321 caaattgtca tggaactttt atctcgaaaa cctaatatta ctaaaaaagc aattattgat
    31381 ggtagtattt gctacccaag acctcttatg gcaaggtttt gtattgctgg tattcgattt
    31441 tttggaggac ttttgtttag tgagaaggct tgtcgctttc aaattgctat gatgccaaaa
    31501 ttgttacctg aaaatatgca atatccagat gaaataaaag catattatat gacagatatg
    31561 ccacatgtaa gaaaagaaac attctataat atgtatcgca cttacatgat gaactataca
    31621 cttaaagaga atgttaaaaa aaccacagca caagtaatgt actggtatgg agaaaaagaa
    31681 atgaaatgtg ttaaaaaatc tgcaaaaatg tttcagtctt atgtgccctc ttgtgagatt
    31741 tatgaagcta agagctataa tcatgggtat ttatcacttt atcttccaaa tgaatggttg
    31801 gaaattgcag aaccattttt ccaaaaggaa taaaggtgtt aggtgattat tgtataatta
    31861 ttgctgtttt tttatatagt aaataaatta ggtataaata taaaaaatta ttttagttaa
    31921 tatattagtg aaagcttaat aggaaaatta taaatagatt aaaggtagcg cctatataaa
    31981 tagttgctac ctttttctgt ttgtaaataa atgatagata agaaggttta ctgacaaatc
    32041 actgactaaa cccaattttc ttacattgaa agccttaatt ttacttgttt taaataaatt
    32101 tacaatttct tgttataaaa tactaaattt tgttaacaat tataattagc atttaattta
    32161 tactggaggt ttatatgatt agtgtaagaa cagatttagc tttagaagct agtgaaatgt
    32221 gtgaaaagtc tcaagaagga agtagtattc ctggtgtaaa aatagaaact aaagaattag
    32281 aaaattgcat tgtaactaaa gttgaagtaa tagacaggca aggcgcagag ataatgaata
    32341 aagatatagg aaagtatata actctggaaa gtaatcttat gaagtttgat gatgatgagt
    32401 caagagaaga aatgataagt tatttaaaag aggagttagt agatatattt ggccaagaca
    32461 aaaataaaaa aaccctggta attggtcttg gaaatagaaa tattacttct gatgcgttag
    32521 gaccaaagag tgtatcaaaa acacttgtaa ctagacatct ttttaaaaat tataataaag
    32581 actatgatga tgatttcact gaggtatcag ctttaagccc aggagttatg ggtgttactg
    32641 gaatagaaac tagtgaaata gtaaaatcat tggttgagaa ggttaagcca gatagagtag
    32701 tagcaataga tgcattggct tcaagaaaaa tggagagagt aaactctact atacagatat
    32761 caacagcagg aatatcacct ggtggaggtg ttggcaatac tagaaaatct ctaactaaag
    32821 aaactttagg cgtggatgtt attgcaatag gcgttccaac ggtggttgat gcagcaactc
    32881 ttacaataga tgtcttagat atggcaatag acaatttaat agctcaatca gaagaaacag
    32941 aaagttttta tgaaatgttg aaaaaactta aagaagaaga aaaatatcat ctaataaaag
    33001 attcgttaga cccatatgac aagaacttaa tagtaacacc aaaggatata gatgatacaa
    33061 tcgaaaatct atctataata ataagtgaag gcttaaatag gtctttacat ccaggtagat
    33121 tggtataaaa taaggaggag caattatgtt taaaaaatgc attaaagtag taaccttgac
    33181 ttttatatta gtatgcattt taccaggaaa atcattagct ttgaatcaag atgatttttt
    33241 aaagttttta gtaaattcat cttatccaga agctaaagtc gaaggaaatg atacagaaaa
    33301 taaaaagaat aataaaaata aagaaacaag taaggaaaat aaggaagaaa gtaaagaaga
    33361 aaacactaaa tctaaagatg caagtaaagt tgataataaa aaagagagtg aaaaagaata
    33421 tataaaactt tatgttggta aagaaaatgt tccagatatt gagtcaaaaa attcagacac
    33481 aactgaaact aacacaacat ctagttcaga ttataaggat gatttaagag taaccaaaga
    33541 aaatccaaga attttgatat atcatactca tggatgtgag acttattcaa attcacctga
    33601 tggaaattat cattcaagag ataagaaaaa ttcagtaatg gaagtaggaa gtgcattaac
    33661 tagtgcttta gatagcaaag gatggggtgt agttcatact actaaatacc atgactatcc
    33721 atcttataat aactcttatg caagtagctt aaaaacaata caaagtatat tacctaaata
    33781 taactcagtg gatatagcga tagacttaca tagagatgct agggatttaa ctaatccagc
    33841 cactaaggaa aaagaccatt taaaatatac aactatgata aatggagaga gagtatcaaa
    33901 gttcttcttt gttgtaggag gtaaaaatac aaatagaaaa caacttagag ctttagcaga
    33961 agatataact gcttttgctg agaaaaagta tcctggtcta gtatctccaa tagtagaaaa
    34021 agactatgct agattcaatc agtttgcagt taagaatcat atgttagtag aaatagggaa
    34081 taacgcaaca agtgtagaag aatctaaggc tactacaaag tatttagccg agattttgga
    34141 tgaatatttt aaacaaaaaa attagagctt taagttataa ttaggagcat gctaaatgag
    34201 ccgattagaa aaaaatatag aaagtaaaag taaactaaga aaatacaagt atattatgag
    34261 aatcttattt ttgattacta tgattatatg tacatcagtt tgtgtattta tagtggacag
    34321 taatgcaaaa agtatgctag gaatgaaaca atatgatttt ccaagtatta aatttgatat
    34381 aacaaaattt gattttgaca taccaaattt tgatatacat gaaatcataa aaaaatttaa
    34441 taagtagtaa taagtgaata aggtttattg attaaaataa gccttatttt agttatatat
    34501 ttttaatgta taaaataaaa attcattaac tgtattttat aggatttact ttaagtttat
    34561 gatattatat aatatttagt gttagacttg ataatccaca tataatgtgg ataattttgt
    34621 ttagtgccaa ttagtttata ttcaaaaatt ttaaatttgg tatataaaga gtttttagat
    34681 aatgtaaaat aataactata agaataatca agaggaggga attttaaggt ggacaataaa
    34741 caaagtagaa ctagaaattt tagtataatt gcacatatag accatggaaa gtctacctta
    34801 gctgatagat taatacaaca aactgggctt gttagtgaaa gagacatgaa aagtcaatta
    34861 cttgacaata tggaccttga aagagaaaga ggaatcacta taaagcttca aaacataaga
    34921 ttaatgtata aagcgaagga tggaaatgaa tattatttaa atcttataga tacacctgga
    34981 catgtagact ttaactatga ggtatcaaga agtttagctg catgtgaagg agcactatta
    35041 gtagttgatg cagctcaagg tgttgaggca caaactttag ccaatgttta tcttgctata
    35101 gaccaagatt tagaaatatt accaataata aataaaatag atttaccaag tgcaagacca
    35161 gaggaagtaa aaaatgaaat agaagattta atagggcttg attcaagtga agctcctctt
    35221 atttcagcta aaactggttt aaacatagaa gatgttttag aagatattgt aaaaaatgtt
    35281 cctccaccta aaggagataa tgaagctcca ttaaaggcat taatattcga ttcttactat
    35341 gatgcttaca aaggtgttgt agcttatgtt agagtatttg aaggaactgt taaaaagggt
    35401 atgacaataa aaatgatgaa taccaataaa aagtttgagg taactgaagt tggtgttatg
    35461 gctccaggtc aaactgaact tagtgaatta tcagcaggtg atgttggtta tattgctgct
    35521 agtataaaag atataagaag ttgtcgtgtt ggtgatacaa taacagattc aaataatcct
    35581 acagaagaac ctttaccagg atacaaaaaa gctactccaa tggtatattg tggtatatat
    35641 ccaggagaag gagaaaagta tgagaatgtt agagatgctc ttgaaaagct tcaagtaaat
    35701 gatgctgcac ttgaatatga agctgagact tcagcagcac taggatttgg ttttagatgt
    35761 ggtttcttag gtcttttaca tatggagata atgcaagaaa gacttgagag agaatttaac
    35821 cttgatataa ttactacagc tccatctgtt atatatagag ttacaaaaat ggatggagag
    35881 gttgtaatga tacaaaatcc tgcaaactta ccagaaccat ctgagataaa gatgatagaa
    35941 gagcctatag taaaaggaga tataatagtt cctaaagact atgtgggtgt tgttatggag
    36001 ctttgccagg aaagacgtgg aaatatgtta aatatggaat atatagatga tagaagagta
    36061 atgcttcact atgaccttcc tctcaatgaa gttgtttacg atttctttga tgctttaaaa
    36121 tcaagaacta gaggttatgg ttctcttgat tatgaggtta aaggttatgt agcatctaca
    36181 cttgtaaaac ttgatatatt aatcaataaa gagcaagtgg atgcacttag ttttattgtc
    36241 catgagacac gtgctttccc tagaggaaag gcaatgtgtg agaaattaaa aggagaaatt
    36301 ccaagacatc agtttgctat accaatacaa gctgctgttg gtaataaggt tattgcaaga
    36361 gaaacaataa gtgctctaag aaaagatgtt cttgctaaat gttatggtgg agatatatct
    36421 cgtaagaaga aacttcttga aaaacaaaaa gaaggtaaga agcgtatgag acaaattggt
    36481 tctgttgaag ttcctcaaaa agcatttatg tctgtgctta aattagatga gtaaatcctt
    36541 ataaatttag ataaagatat ttaaaagcta gagtgatttt agaattattt ctaaattagt
    36601 ctagcttttt tatggtatat tttaagtaaa ctcatagaga atataagtaa gaatattaaa
    36661 gaagaataaa aaggtgatat ttatgattaa tatagaaatt cctaaaaaag tagactatat
    36721 aattaaagaa ttagaaaaga atggttatga agcttatata gttggaggat gtgttagaga
    36781 ttgtttatta gaaagagttc caaacgattg ggatataaca actagtgcta gaccagaagt
    36841 ggtagtagaa ttatttgaaa aaacaattcc aactggtata caacatggga ctgtaacagt
    36901 tatgattgaa catgaaccat ttgaagtgac aacttataga attgatggaa attatagtga
    36961 tggtagacat ccagattcaa tagaatttac aaataacata gttaaggact tgtcaagaag
    37021 agattttact atcaattcaa ttgcatacaa ttcaaagaca ggattagtcg acccatttaa
    37081 tggctatgaa gatatacaaa ataagtatat acattgtgtt ggaaatccag tagatagatt
    37141 tgaagaagat gcacttagaa tgcttagagc agttagattt tcagcacaac tcaattttaa
    37201 aatagctgaa ggtactaagc aaagtattca taaaaaagca gatttgataa aaaatgtttc
    37261 tattgaaaga atccagacag agtttaataa aatattagta tctgattcat ctaaattaaa
    37321 cttattaaaa tcaacaggtt tgttgaaatt tataattcct gaaatatgtg aattagaaga
    37381 tgttactcaa cacaatccat atcacatcta tgatgtacaa aaacatactt taatagctac
    37441 agaagcaatt gaagatgaac tgtatttaaa acttacaatg ttgtttcatg atttaggtaa
    37501 aaaagtaact aaaacaactg ataaaaatgg tgttgaccat ttttatactc atagtagaga
    37561 atctgtaaag attgcaaaga agattttgaa aagacttaag tatgataatt atactattaa
    37621 taaagtttta atattaatac aatatcatga ttatagaata gaacctaaaa ggaagataat
    37681 taaaaagtta ttaaataaac ttgaagatgt tggattattt gaagatttaa taaaagttaa
    37741 ttgggctgat acacttgcaa aaaatccaaa gtatgcaaaa caaaagatat taaatcttat
    37801 tgagtgtgaa aaagaattta aacatataat aaatcaaaag gagtgcttta atcttaaaga
    37861 tttagctatt aatggaaaag atttgatatc tataggtata aaaccaggta aagatatagg
    37921 atatatttta aataagatgc ttgaaatagt cataaataat ccagagttaa atgaaaaaga
    37981 aatactaaaa gaaaaagcat taaatatata tacattttag attcaaatat aaaaaattta
    38041 attactttaa aaaacataga ataaaatact tatttagata aaataaataa tttttagaga
    38101 atattttaga agttacgatt gtatgatttt taaatgcata ggcatttact ctaggactat
    38161 agactaatat aaaaaaaaac tttgggaata ctaaaaccat aaatttataa aattaatatt
    38221 ttatatactg taaaacagtg aggagggaca gtatgcatac tcaactacaa aagactattg
    38281 gtctttcagc agctctttct actgttgtag gtatggttat aggttcggga gtttttttta
    38341 agccacaagc aatatacact acaactaatg gagctccagg tctaggtatt atagcgtggc
    38401 ttttaggtgg ttttataaca ataactgctg ggcttacagc tacagaaatt tcagctgcta
    38461 taccaaaaac tggtggtatg atgatttata tcgaagaaat atatggagaa aagttaggtt
    38521 ttttaactgg atggatgcaa actgtattat ttttcccagg tacatcagcg gcattaggag
    38581 taatatttgc tcaacaagca tctgaacttt taggaatgag cccaaataat atggcaaatg
    38641 tacttcctat agcaattgga gttatactat ttttagcgtt attaaatata ataggttcat
    38701 ccttaggagg aaaagttcag acagtagcca ctattggaaa gatgatacct ttaattttaa
    38761 taatagtctt tggatttatt aaaggtcaaa gtagtgaagt attaaatcca tttgtaggag
    38821 atggtgtaaa tgcatcaaat gcacttggac aagtattaat agctactttg tttgcatatg
    38881 atggttggat aaatgttgga gctatatcag gagaaatgaa gtctccagag aaagatttac
    38941 caagagcaat agttggtggt ttatcattag ttatggcagt ttatattata ataaatatag
    39001 cttatctttg ggtagtacca gcaagtgaat tagcaacagt tacttcccca gctacattag
    39061 tagcaacaag attgtttgga aatataggtg gaaaagtaat aactgtgggt atattaatat
    39121 cagtatttgg gacattgaat ggatatttat taacaggttc aaggatacct tatacattag
    39181 ctgaaatggg aactttacca gcatctaaaa ctcttttaaa agtaaattct ggaggttctc
    39241 ctgtaaattc aatactactt ataactgtgt tagcctgtgt atatgcatta tctggtcaat
    39301 ttaatctttt gacagactta acaatatttt caatatgggt attttatgta ttgactttta
    39361 taggtgttat gaggcttaga agacaaaagc cagatttaca cagaccatat aaagtccctt
    39421 tatatccaat aataccaatt atagctatat taggtggact gtttgtaata ataaatcaaa
    39481 tattgacttc aacagtcata tctttaggcg gaatatttat aacactatta ggtttaccag
    39541 tttattatta tatgaaaaaa agataactac ttaatttaag ttagataaat ttgggtaaag
    39601 gtgaaaatta acttttatcc aaatttttta tactgttata caatataagt attatatatt
    39661 gcttgatgtg ctattttgtt tgatttataa tttagtaatt gatgttttaa gataatttaa
    39721 gaaatgagga gtgaaagatg ctaggacttt atgtacatat tccattttgt gtgaaaaaat
    39781 gtaagtattg tgattttaat tcttataaaa tggacataga ttcaaaaaaa agatatatag
    39841 aagacttaaa aatagaaatg gaattatata gtaataaatt atataaagat aataaatata
    39901 aaaataaaga atgctgtagt ctaaataaaa atgacaaaat aacgagtata tttgtagggg
    39961 gaggtacacc tagtatacta acctctgatg aaataagaga agtatttata agtataaaag
    40021 aaatgtttga tatagatgag aatgcagaaa taactataga gtgtaatcct gggacattaa
    40081 cattagaaaa attaaaaact atgaaagaga ttggcataaa tagacttagt atagggctac
    40141 aagctattca agaaaagcat ttgaacttta taggaagaat acatacttat gaagaatttg
    40201 agaaaaacta taaagatgca ttaagtgtag gttttaaaaa tattaatgta gatttaatgt
    40261 acagtcttcc aaatcaaact ctatgtgatt ggaaggaaac tcttgaaaaa gttgtacact
    40321 taaatccaac acatatatca gcttattccc ttatactaga agaaggaact gaactataca
    40381 atatgtatga aagtaataaa tttgaactta tagatgaaaa tgtagatata gaaatgtatg
    40441 agtatacaat aaactactta aagtcaaaag gatataatca atatgaaata tcaaactact
    40501 caaaagaagg ctataattgt gagcataata ttttatattg ggaatgtgaa cattatatag
    40561 ggattggagc aggagcttct ggctatatta atgagaacag atacaataat gtagaatctt
    40621 tagaagacta tcatttaagt ttggtaaaaa gagaaaaacc aatacaagaa aatgaaattc
    40681 tttctgagaa agatatgata gaagaaaaga tatttatggg gcttagaatg aataaaggca
    40741 taaaatttga agattttaaa aaaaaatttg gaatagattt tagagagaag tataataagc
    40801 aaatagaaat gctattagct agaaagctta taaatcagag ctttgaaggt atacaattaa
    40861 ctcaaaaagg aagagaaata tctaatagtg tatttataga atttatggaa taatttttaa
    40921 ggtgttgaca aaataaattt ataatggtat ataatatata taatcttttt agcactcgaa
    40981 cttagtgagt gctaacagct aggaggtata ataatatgga attaaatgaa agaaaattaa
    41041 atatcctcaa agctatagtt aaagattata tagaaacagc tgaagctata ggttctagaa
    41101 caatatctaa aagacatgat ttaggtgtta gtgctgctac cataagaaat gaaatggctg
    41161 accttgaaga attagggtat ttaattcagc ctcatacttc tgcgggtaga gttccatctg
    41221 aaaaaggcta taagttatat gtaaactctc ttatgagtaa aagcgaattg gatgataatg
    41281 ataagattct tattgaacaa tgtatgaatc acaatataaa tcatataaaa gaattgattc
    41341 atgaaacttc taagctatta tcgcaactaa caaactatac aactgtagca gttactaaga
    41401 gtttaataaa tcaaagtgtt ataaagcaca tacagttggt tgctatgaat gacaataata
    41461 ttttacttat agttgtaaca gataaagggg acttaaaaaa agctaacctt acaactaatg
    41521 tatatttaga tcaatctaag ttaaatttga tttctgataa tcttacaagg aagctcttag
    41581 gtaaaagtat aacagatttg gatgataatc taattgcttt tattaaatac gaaataagtg
    41641 agtattctgg gcttatagat gagcttttaa atgctttgaa ctcaaatatg aaagaagaag
    41701 atttttctct atcattaaat ggagctacta acatatttag ttatccagaa tttaatgatg
    41761 tattaaaagc aaagtcattt ttaaatatgt tggaaaaaaa ggaaacaata gctgacataa
    41821 ttaaatcaaa gggaatacaa aaagacaatc taaatataat aattggtagt gataatgatt
    41881 gtgaactggc acaggattgt agtatagtta cagcaactta taatgtagat agagacttgg
    41941 ttgggcgaat aagctttata ggccctacca gaatggatta tgctagaatt tactctataa
    42001 taaattatat gagtttatta attaatagaa agtaaagaaa ttttaaattg cgaggtgtaa
    42061 agtcttggat aaaaaaaatg agcaacagga agtaagagaa gaaaatgata cttctattaa
    42121 ccaagaatcg gaaactcaag tagagttaga agaggaagtt gttaatgaag aatgtgaaac
    42181 atcaagtgaa aaaactgatg aaaaagaagt tgatgatgaa aatgtaacag atataaattc
    42241 taagttagca gaaaaaaaat tgcaggatga gttggatgaa ttaaatgata aatatcaaag
    42301 acttcaagcc gaatatgcaa actacagaag aagaactcaa caagagaaag agacaatagg
    42361 cgtatttgca aatgaaaaaa taataactga attgatacca gttatagata gtatggaaag
    42421 agccttagat gcatgtgaag ataaggaaga tactatgtat aagggtataa gccttgtaca
    42481 taaacaatta atagacactt tagtaaaatt tggagtagaa gaaatagaag cagaatctaa
    42541 ggaatttgac cctaatctac atctagcagt tatgcaagaa agtgtagatg gagttgaagc
    42601 aaatcaaata gtaatggtac ttcaaaaagg atataagcta ggaactaaag ttgtaagacc
    42661 ttcaatggtt aaagtttctt gctaagtata aattaaaggt taaattattt aacctaatat
    42721 aaaatattaa aataagtaaa acttaattat ataattactc gaggaggatt ttagtcatgg
    42781 gaaaaataat aggaatagat ttaggaacaa caaattcatg tgttgcagtg ttagaaggtg
    42841 gagaagcaca aataatagca aatagtgaag gtatgagaac tactccatca gtagtagcat
    42901 ttacaaaaga tggtgaaaga atagttggag aacctgcaaa aagacaagca gttacaaatg
    42961 cagataaaac aataacttct ataaaaactc atatgggaac tgattataaa gtaaatatag
    43021 atggaaaatc atatactcca caagaaatat cagcaataat attacaaaaa ttaaaatcag
    43081 atgcagaaag ttacttagga caaacagtaa cagaagcagt tataacagta ccagcttact
    43141 ttacagatgc tcaaagacaa gcaactaaag atgctggtag aatagcaggg cttgatgtta
    43201 agagaataat aaatgagcca acagcagcag ctcttgctta tggtatggat aaattagacc
    43261 aagaaaagaa aatattagta tttgacttag gtggaggaac ttttgacgta tctatacttg
    43321 aaataggaga tggaactttt gaagttttag ctacagctgg taacaataga ttaggtggag
    43381 atgactttga ccaaatagta atagattatt tagcagagga atttaaaaaa gctgaaggtg
    43441 tagatttaag aaatgataaa atggctcttc aaagattaaa agaagcagca gaaaaggcta
    43501 agaaagaatt atcatcaaca atgagttcaa acataaactt acctttcata actgctacag
    43561 cagaaggacc aaaacactta aatatagatt tatcaagagc taaatttgaa gaattaacta
    43621 gaggtttagt tgaaaaaact atggaaccaa ctaagacagc tttacaagat gcaggacttt
    43681 ctacaggtga tatagatgat gtattattag taggtggttc tacaagaata ccagcagtac
    43741 aagaagctgt taagaagttt ataggaaaag aacctcataa aggtataaac ccagatgagt
    43801 gtgttgcagc aggtgcttct atacaagctg gagttttagc aggggatgtt aaagacttat
    43861 tattactaga tgttactcca ttatctcttg gtatagaaac tatgggtaat gtaatgacta
    43921 agataataga aagaaatact acaataccaa ctaagaagtc tcaaatattc tcaactgctg
    43981 ctgataacca aacagctgtt gatatacatg ttcttcaagg tgaaagaagt atggcttatg
    44041 acaatacaac tttaggaaga ttccaattaa cagatatacc accagcacaa agaggaatac
    44101 ctcaaataga agttactttt gatatagatg caaatggtat agttaatgtt tcagctaaag
    44161 atttaggtac aggaaaagag caaaaaataa ctataacatc aaacactaat ttatcagaag
    44221 cagaaataga gcaaaagata aaagaagcag aaatgaatgc agaagctgac aaacaaaaga
    44281 aagaaaaaat agaagcattc aatcaagctg aatctactat atatcaaact gaaaaaactt
    44341 taaatgaact tggagataaa ataagttcag gtgaaaaaga ggatatagaa aaagctatag
    44401 cagacttaaa agctgtaaaa gataatcaag atgctacagc agaagaactt aagaaagcta
    44461 cagatgaagt aatgactaag ttccaaaaag tatctcaaga aatgtaccaa aaagcagctc
    44521 aagaacaaca agcggcacaa ggtgcagaac aagcacaaga caatggacct aaagatgaca
    44581 atgtagtaga tgctgacttt aaagaagtag atgaagataa ataagctata taatatgtaa
    44641 cactaaatga taattggaac atatatgatt tgtgaaaatc atatagatgt taaagatata
    44701 tggacaatat acatcataaa gaatataata tatcatagat tatataactt aaagtagaat
    44761 ataagaatta atgatagctt gtagtttatt attctacaag ctatcgtttt gtaaaatgaa
    44821 ggtggtgtca atttttgagt acaaaaaggg attattatga agtcttaggt atcagtaaag
    44881 gtgcagaagc tcaagagata aaaaaagctt atagaaaatt agcaatgaag taccatccag
    44941 atagaaatcc tggagataaa gaggcggaag aaaaatttaa agagataaat gaagcttatg
    45001 aagttctatc tgatgataca aagagaaaaa cttatgacca gtttggacat gatgggctaa
    45061 atggtcaagg cggttttggt ggtcaaggag gatttggcgg tcaaggcttt gggggttttg
    45121 aagatatgtt cggagatata tttggtgaca tgtttggagg aagcttcgga ggtggaagag
    45181 caagaagaag agggcctcaa cgtggtgcag atataagaca gagtgttact atatcttttg
    45241 aagaagctgc atttggtaaa aagatgtcta taaaagtaaa tagaagtgaa gaatgtgaag
    45301 aatgtaatgg aactggagca aaaccaggaa catcaaagaa aacttgttca acttgtaatg
    45361 gaactggtca agttagaact gttcaaagaa ctccatttgg taatatagca agttcaagac
    45421 catgtagtgc ttgtaatgga actggagaag tgattgaatc tccatgtagt aaatgtcatg
    45481 gaactggaaa tacaagaaaa gttaagacta tagaagtaga tatacctgct ggtatagatg
    45541 atggtcaaat gataaaactt tctggtcaag gtgaagtagg agaaaaaggt gctccaagag
    45601 gagacttata tattgtagtt aatgttaagt cacatccact atttactaga gatggaaatg
    45661 atatttattt tgaaatgcca attacatttg ttcaagcaac tttaggtgat gagatagaag
    45721 tacctacttt ggatggaaaa gttaaatata gtgtgccaga ggggacacaa actggaactg
    45781 tatttagact taaagagaaa ggaatcccta gaattagagg taattcaaga ggagaccaat
    45841 atgttaaggt tgtagttgaa attcctaaaa aattaaacga taagcaaaaa gaactattga
    45901 gagaatttgc gaaggagtgt gggtcgaacg tccacgaaaa gaagaaaacc ttcggacaaa
    45961 agatagaaga tatgtttaag aaaaagtagt taaagtagcc aatatataat catattttta
    46021 aaatcttagt tatttataag attgtcaaag gagatattat gatttttata ttggctattt
    46081 ttgtataaaa aataaatgtg cataaaataa aactgaatat aaataatttt ctgaaaaata
    46141 cttgtcctaa aatatataat atgctataat ataatcaaat aaaagaaagg ggtgattcca
    46201 gtgggaacgg atgtggcact gccaaatgat gaagaaaact ttattgtaat atacaaaata
    46261 aaattagata cttttagtgc tagattaagt actggaaaaa ctcatgagct gggataatta
    46321 ttgtaattac ctagactcaa acttttagta gtaaaatagt aatattatat agacaaggta
    46381 tctaattttt aaatcttttg tatgaattaa aatttttata aagggtatat ctgattagaa
    46441 tgtaattagg gaaacctagt tgtgaattac ttttagtata tacatgtgta aagaaatatg
    46501 tatatggtat tgggtattgt gctagatgtg tatgtaaagg aatatgtgtc tagttgggta
    46561 tatgtaaaga aatatactta taatctatga aaaattttta gtataaattg ctaaaacatt
    46621 gagattgttt tttatagaaa gttagtttat accaactaaa aatgtgctta agatttgttt
    46681 gatgtttaat tataaataag gttgtagata aaaaaataag taaagacaaa ggtttaaaat
    46741 acttgcttta aatatagttt gttttgaaca cttgccaaat taaaataatt aataaaatag
    46801 acatcttctc atagtaaaat atactgtgag aggatgtttt tgctttgttc ataaactttt
    46861 atgaaaaatt tgcatagtgt cacattttaa tatactatat atataataaa atatagataa
    46921 aattaaatga ttcaaaagag aaaggtgaaa agagatgtat tatataggtg tagacatagg
    46981 aggaacaggt atacaagcag gagtagttga taactatggg aagataattt ttaggtcaga
    47041 gtgtaaaaca gtaattgaaa aaggatttga aggaatactt aatgacataa aaataatgat
    47101 ttataagttg ttggaagata ataaacttac tatgagtgac ataaaatcta ttggatttgg
    47161 tgttccaggt tttataaata aagaaggatt agtaacttgt gtaaatttaa aatggaataa
    47221 aaaagctttt aataaagagt taaaaagaag atttccagat gttgaaattc atggagaaaa
    47281 tgatgcaaca gtagcagcac ttggagaagc aaagtttgga agtatgaaag gtgctaatgt
    47341 tggagtatta tatactttag gaactggtgt aggtggagga atagtaatta atcaaaaagt
    47401 cttttctgga gcacatggat tgggttcaga aataggtcat caaattatag gagaaaacta
    47461 ttttaattgt aattgtggaa ataatggttg tgtagaaact ttttgttcag caactgcaat
    47521 tataaagtat tctcaaaaac tgatagaaga aggagaaaag agtaggattt tagatttggc
    47581 agagggaaat ttagaaaatg taaatgcaaa gatggtgttt gatgcatata gagaaaatga
    47641 tttagtggca attaaagtaa taaatagatt taaagagtat ttagcaaaaa cttttgcaaa
    47701 cacaataaat tctttagacc cagaaattat atctatagga ggaggcattt ctaaatctag
    47761 cgatataata cttgatggaa tagaaggttt ggtaagaaaa tttgtattat acaaaacaga
    47821 agatatagct acaattacat gtgcaacttt aggttcagac gcaggaatta taggagcagc
    47881 ttttttataa tgtaaatgaa ctgcttattg gaggcgtgat gatatatggg taaaacaaat
    47941 gctagaagaa cgcttttttt gatagcaata ggttcaggaa ctatgctgaa tcctcttaat
    48001 tcttctatga tttcacttgc tttacatagt atacaaaatg attttcatat tagttttaca
    48061 actgtatctt ggattgtttc agcattttac ctagctagtg ctattgcaca accagtttca
    48121 ggtaagatag gagatttaat aggtagaaag gtcttatttt tgagtggttt gatgcttgta
    48181 attttatcag catccatagt accacttgtt caatcattct ttatattgat ttttataaga
    48241 gtaattcaag caattggaac aagtacccta tatccatctg gagttgctct tatacaaaat
    48301 aatataaaag aaagacaatc ttctgcatta gcagttctaa ccatttttgc ttcaactaca
    48361 gcaggattag gtccgacact tggaggatta ttgttggact taggaggatg gcatgctata
    48421 tttcttgtaa atatacctgt tgttttggtt tctatatgct tagggtattt tttattccca
    48481 aaagaagtaa aagaaaagaa atctataaaa gaaactttaa aaaatgtttt gtcaagatta
    48541 gatattattg gcatacttct attttcaatt gcaatgattt ttatattact atttttgcta
    48601 tctatgaagg aatcttttaa cttagaacaa cttgtatttg gtataatact gatgtgtctt
    48661 tttatttggc atgaattgag aacaaaattg ccatttatag atattaagct ttttgtaagt
    48721 aatcctaaat tgtcaaaagt ttacttgcaa tttattatat taaatttctt taattatatt
    48781 ttgttttttg gtcttcctag ttattttcaa gatgccttac attatagtgc aaagtcgact
    48841 ggattattta tgctatttat gtctggtata ggtatattta tatctccatt aactggaaaa
    48901 tggatagata aatctggaac tagatttcct cttgttacat catcaatctt tatgtttatt
    48961 agtgcagtac ttctttcagt agtttttatc catatacctg taataggaaa gggtattata
    49021 ttatcattag caggtattag ttatggagtt ggtaatgtag ccctgcaatc ctctatgttt
    49081 gaagaaagtc ctcgtgacag tatagggaca gcttctggtc tgtttcaaac tagtcgttat
    49141 ttaggttcta ttttagcaac tgttttactt ggaatggtat ttgaagtaaa tataacatca
    49201 gaacaatttc agatattagg ttatgttatg gtggtcttag gtacaataag ttttttatta
    49261 aattttatat taaagaaaga attaagaaaa gtagaatagt ggatttatgg atttaataag
    49321 tctaataact gataagttta aaaatattgt ttgtaaaaca taactggttt gatatttatt
    49381 aattctaagt tttgaagcat agagtactcg ataagtatac aatattttga attaaaatta
    49441 agcagaaatt tgaactagtc tttcaaatct ctgcttaatt tagtagttaa atttttttat
    49501 agagaaaata agatttgaca cttagataaa aaatcattgc attttcaaaa ttctttaaat
    49561 caaacccaag catctcatta atcttttcca atctatatac tagcgtgttt ctatgtaaaa
    49621 acatactagt agatgtctta gaaatattta aatcattatt tataaatttt attcctgtat
    49681 atagaagttc ttcgtcaata gatttaatct tattaaaatc tattaattta ttgagtatat
    49741 tatcctcatt aattaaatct aaagttttat atctaataag gtcaaacaaa tttaaaaact
    49801 tattttcagc gttgtctttt ataatatgag gtagtaaatc agaacataac tgtaatctaa
    49861 aatcaaaatc agctttacta tctaatttac caagataaaa aatataagta ctttgataag
    49921 tttcagtttc aattatattt tgtgtagtta cattaatatc taagttatat ttagaattta
    49981 ctataaaaaa accattgtgt tgtattatat ttatatcttc aaataaatta taaaaaatat
    50041 tttttagata ttcaaagtct atatgagatt ggtaaattaa ataaaagttg ccttttaaat
    50101 tatcaagatt atttttatct agaaaatcaa taacataaat agcattattg aaagtaacta
    50161 aatggctact agcatctaac ttttggtttt catatggata gatatttatt tttttattaa
    50221 gctgtctttc taaaaaatct tttaactctt ccataaagtc tccttaaaaa taagattata
    50281 ttcttatctt atagctaaag tagtttcttt gtcaaatatg tgtactctac tgttgtcaac
    50341 tgaaagtgtt aagtcatcac caacattaat tctgttttta ctgtcaaatc taattgttat
    50401 cgtattttcg cctagtttta agtgagcata agtttctgct cccataagtt cttttatttc
    50461 aattcttgat ttaaaacaag tatctggatt agatttaata aaagtatctt ctacatgaat
    50521 atcttctggt ctgataccta taacaacttc attattaata taattgtctt ttattaaatc
    50581 atagtgtttt tctttattca atttaagctt aatatgttca ttttgtgcat aaatttcatc
    50641 attttcttct attaatatta catcaataaa attcatctgt ggtgctccaa taaatcctgc
    50701 aacaaatata ttagctggat aatcgtatac atcttgtgga gttgcaatct gctgaactac
    50761 tccatccttc attactacga ttctatcagc catagtcata gcttcaactt ggtcgtgtgt
    50821 tacatagata aatgtagttc caagctgttg gtgtagctta gttatctctg ttctcatagc
    50881 agttctaagt tttgcatcta agttggatag aggttcatcc attaagaata ccttaggatt
    50941 tctaactatt gcacgtccta aggcaaccct ttgtctttgt cctccagata atgttttagg
    51001 ttttttattt aaaagaggta gtaagtctaa tattttagca gcttctttaa cttttttgtc
    51061 tatttcatct ttaggcaatt ttctaagttt aagtgcaaat gccatgtttt cgtaaacaga
    51121 taagtgggga tataaagcat agttttgaaa aaccatagct atatctctat cttttggttc
    51181 tatatcattt acaagtttat ctccaatgta tagttctcct tcagagattt cttcaagacc
    51241 agcaatcatt ctaagtgtag tcgatttacc acaaccagat ggtcctacta aaacgataaa
    51301 ttctttatct ttaatgtcta tatttatatt ttttactgca ttaaatccat ttgagtaaag
    51361 ttttgatata ttttttaaaa ttacttctga cataatatcc tccttgatat ttaaatttta
    51421 attatatagt atcaaatttt tttattatat taaatgtgta aaatgactaa atgtatttgt
    51481 ttaatatgta tattaaaaat ttagactaat agttttttaa tttaaaaaat gtattttaat
    51541 ttagaaaagt tttatgtata atagtata
//
LOCUS       DAMJYC010000022        51357 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJYC010000022 DAMJYC010000000
VERSION     DAMJYC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.023"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(68..679)
                     /locus_tag="PC472_001336"
     CDS             complement(68..679)
                     /locus_tag="PC472_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2726669.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(771..1760)
                     /locus_tag="PC472_001337"
     CDS             complement(771..1760)
                     /locus_tag="PC472_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2726670.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1827..2690)
                     /locus_tag="PC472_001338"
     CDS             complement(1827..2690)
                     /locus_tag="PC472_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2726671.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2796..3392)
                     /locus_tag="PC472_001339"
     CDS             complement(2796..3392)
                     /locus_tag="PC472_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2726672.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3432..4235)
                     /locus_tag="PC472_001340"
     CDS             complement(3432..4235)
                     /locus_tag="PC472_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2726673.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(4271..4711)
                     /locus_tag="PC472_001341"
     CDS             complement(4271..4711)
                     /locus_tag="PC472_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2726674.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(4724..6826)
                     /locus_tag="PC472_001342"
     CDS             complement(4724..6826)
                     /locus_tag="PC472_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2726675.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(7087..8790)
                     /locus_tag="PC472_001343"
     CDS             complement(7087..8790)
                     /locus_tag="PC472_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2726676.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(8816..9775)
                     /locus_tag="PC472_001344"
     CDS             complement(8816..9775)
                     /locus_tag="PC472_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2726677.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(9911..10429)
                     /locus_tag="PC472_001345"
     CDS             complement(9911..10429)
                     /locus_tag="PC472_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2726678.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(10472..10957)
                     /locus_tag="PC472_001346"
     CDS             complement(10472..10957)
                     /locus_tag="PC472_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2726679.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(10954..11571)
                     /locus_tag="PC472_001347"
     CDS             complement(10954..11571)
                     /locus_tag="PC472_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2726680.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSISDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(11636..11998)
                     /locus_tag="PC472_001348"
     CDS             complement(11636..11998)
                     /locus_tag="PC472_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2726681.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(12742..13239)
                     /locus_tag="PC472_001349"
     CDS             complement(12742..13239)
                     /locus_tag="PC472_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2726682.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(13414..14325)
                     /locus_tag="PC472_001350"
     CDS             complement(13414..14325)
                     /locus_tag="PC472_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2726683.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            15295..16146
                     /locus_tag="PC472_001351"
     CDS             15295..16146
                     /locus_tag="PC472_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2726684.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            16150..16620
                     /locus_tag="PC472_001352"
     CDS             16150..16620
                     /locus_tag="PC472_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726685.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            16633..17943
                     /locus_tag="PC472_001353"
     CDS             16633..17943
                     /locus_tag="PC472_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726686.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            18155..18901
                     /locus_tag="PC472_001354"
     CDS             18155..18901
                     /locus_tag="PC472_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2726687.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            19263..20804
                     /locus_tag="PC472_001355"
     CDS             19263..20804
                     /locus_tag="PC472_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2726688.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            20839..21945
                     /locus_tag="PC472_001356"
     CDS             20839..21945
                     /locus_tag="PC472_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2726689.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(22155..23441)
                     /locus_tag="PC472_001357"
     CDS             complement(22155..23441)
                     /locus_tag="PC472_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2726690.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(23564..26662)
                     /locus_tag="PC472_001358"
     CDS             complement(23564..26662)
                     /locus_tag="PC472_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2726691.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            27387..27545
                     /locus_tag="PC472_001359"
     CDS             27387..27545
                     /locus_tag="PC472_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726692.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(27832..28536)
                     /locus_tag="PC472_001360"
     CDS             complement(27832..28536)
                     /locus_tag="PC472_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726693.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(28527..30134)
                     /locus_tag="PC472_001361"
     CDS             complement(28527..30134)
                     /locus_tag="PC472_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2726694.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(30388..31566)
                     /locus_tag="PC472_001362"
     CDS             complement(30388..31566)
                     /locus_tag="PC472_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726695.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(31792..32286)
                     /locus_tag="PC472_001363"
     CDS             complement(31792..32286)
                     /locus_tag="PC472_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2726696.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(32287..33048)
                     /locus_tag="PC472_001364"
     CDS             complement(32287..33048)
                     /locus_tag="PC472_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2726697.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            33770..36604
                     /locus_tag="PC472_001365"
     CDS             33770..36604
                     /locus_tag="PC472_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726698.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFSETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            36647..37891
                     /locus_tag="PC472_001366"
     CDS             36647..37891
                     /locus_tag="PC472_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2726699.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(38306..40048)
                     /locus_tag="PC472_001367"
     CDS             complement(38306..40048)
                     /locus_tag="PC472_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726700.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(40032..40706)
                     /locus_tag="PC472_001368"
     CDS             complement(40032..40706)
                     /locus_tag="PC472_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726701.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(40743..41732)
                     /locus_tag="PC472_001369"
     CDS             complement(40743..41732)
                     /locus_tag="PC472_001369"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2726702.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(41780..43024)
                     /locus_tag="PC472_001370"
     CDS             complement(41780..43024)
                     /locus_tag="PC472_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2726703.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(43066..45612)
                     /locus_tag="PC472_001371"
     CDS             complement(43066..45612)
                     /locus_tag="PC472_001371"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726704.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(45820..45981)
                     /locus_tag="PC472_001372"
     CDS             complement(45820..45981)
                     /locus_tag="PC472_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726705.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(46037..47053)
                     /locus_tag="PC472_001373"
     CDS             complement(46037..47053)
                     /locus_tag="PC472_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2726706.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(47050..48348)
                     /locus_tag="PC472_001374"
     CDS             complement(47050..48348)
                     /locus_tag="PC472_001374"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2726707.1"
                     /translation="MIDSEEMIFNVEDLIIYRKNQLVIIYNKKFRKYIKLSEEIYKYF
                     LLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGVAIYFC
                     LTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFF
                     EIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKG
                     VFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGR
                     GLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDV
                     YPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIF
                     CWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
     gene            complement(48814..50133)
                     /locus_tag="PC472_001375"
     CDS             complement(48814..50133)
                     /locus_tag="PC472_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2726708.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNSIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(50127..50255)
                     /locus_tag="PC472_001376"
     CDS             complement(50127..50255)
                     /locus_tag="PC472_001376"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726709.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(50394..51104)
                     /locus_tag="PC472_001377"
     CDS             complement(50394..51104)
                     /locus_tag="PC472_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726710.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT        17307 a         8491 c         5474 g        20085 t
ORIGIN      
        1 taaaataaac tatcttccaa acattttatt atatgcttaa aagatagttt tgtgtcttta
       61 aaataaatta aattgtatat attccttcac ataacttttg tatattacta ggtggttgcc
      121 atttaccatc ttttttagta tatgtaatca tagttgtaac ttccttagtt ggtttcttat
      181 tgtaaaggtc tataatcatt tttgtcatat catcttgaac ttctttttct tttttatcgt
      241 aattcttttc atcagttaca taagatttta ctaaagtgtt cgcttcactc attactttac
      301 tagtatctaa agctttactt acaactttca aagttatctt atccttacta gaagattcca
      361 ctgttactgt cttttcaaca ccttgaatta atttaacaat tgtttcgtat atcttttcag
      421 cattttcttt accataaaga ggtgtcaatt gagcaatcat tgaaaccttg tccttgctgt
      481 tataagtttg aatctgttta tattcagctt ctgttaatgc atcagaaatt cctttttcat
      541 catacttata catttctaat gcagctaaat tagcttcaaa attcgacttt actttttctt
      601 ctgcattaga acaccctata gttagaacta aaactagagc cataatcgta gataatatta
      661 acttttttac cattttcata caaacatcct ccctcaatta aaataataaa aaatttattt
      721 atcaacatta taacataaat ttgtctaact atagaattat tttgaatatt ttaccacttc
      781 cagtctattc caataccgtt ttcttttgct ttatcgtata ttctttttgc tgttacgaca
      841 tcttgtacac ctatcccaac agttttaaat acaattatct catcatcatc ttctcttcca
      901 ggaatagttc cgtttataac atttcctaaa tcaccactaa aatcatcttt acttatctta
      961 ccttctttta atggaattaa tatatctcct gactcagata aaactgcatc tgttgattca
     1021 aaaaatatct tagacgctct agttaaacag tttgggcaca actcttgcat atctggcata
     1081 tatgaaccta ctgcacttat taaagctcct tttttcacta agtttccatt aagtactggt
     1141 tgttttgatg gagtagctaa aactatcaca tctgcgttat ctatggcttc gtcagatgat
     1201 tcaactgcaa ctattttagt attatattta cttaatttta agttcatttc ttctgcaaat
     1261 ttttctctac tttccttgtt tctactaaaa actcttacct catccaaatt tctagcttct
     1321 atcatggctt caagttgtgg ctcaccttgt cctcctagac ctatcaaagc tccaatctta
     1381 gaatcttttt tagccaatac gtctatagct gctccagagg ctgctccagt acgaatctga
     1441 gttacataag taccatccaa tactgcacta acttctccag tttctccatc taataatagt
     1501 actgttgctg gaattactgg tttgcctttt tgagcatttt taggaaatac agatacaatt
     1561 tttattcccc cacagcctaa ttcttctacg tatcctggca taaataacat actagcatct
     1621 tgacttggaa cagatatatt ggttctcaaa ggatttacac tctttccttc acaataaatt
     1681 ctaaaagctt ccttatcagc ttcaattgca tctctcattg taaatacttt ttttatgtcg
     1741 tcttttttta aaagtagcat ttttataccc ccaattttaa taagtctcta tgctaaatat
     1801 tatagcataa catttaattt aatcttctat ttcaaatcaa ataaatctac atactctggt
     1861 tttagcatag aacttatcaa cgctttattt attgaaaatt ctggtattcc tgctgcatat
     1921 ggagttatat catacaaatc aaaatataca actagattct catcttgcaa gtaaaagttt
     1981 tgattctctt ttataccttt aaaattatat attccgatat tttcttcatc ctttttttca
     2041 agttcagcta tttgtctttt aatttcttca tctataactt ctttatattt agtattatcc
     2101 ttaaacaagt ccattaattc taaaaatttt ccagttctca tatccacatt atatgctata
     2161 tcttgataaa atccatgtgc tccaccacta taagtataat atctcacttt tatactaaga
     2221 gcagaatcag tattcttttt aagttcaaag tctacatttg caacaaactt atttttctcg
     2281 tcaggattat ccttaagata ctgctttgct tctttatatg atttattata aaattcaaat
     2341 atgtcatttt ttatcttatc atttgtagac ctttcaacaa ttttatttga atttgttatt
     2401 acaggcatgt ttatagtact ttctaaatac tcatcatttt tcactattgt tttacttcct
     2461 attgtactta ctgataaagt ttgtttacta tttgtattac cctttggtaa tgactggtta
     2521 ctcataacag tcactgcttc cttattacta tttaaattaa ttgaagcatc tttccttaaa
     2581 ttattatatt tatagtcatg tttaattgcc aaaacagcca ttatagtcaa tgttaaaaca
     2641 aatacatatc ttaaaacttt agcttttgta tcgactttaa aaaatttcat attatcattt
     2701 tcctcctaaa tataaatctc tcttattata tttggagttt ctagggtttt ttatccatat
     2761 aatttaataa aagattaatt tttatttata aaaaattaat ctttttgttt taaaatatta
     2821 atatgtctta tcaaacagtt tgaaatttca ccaagaggta attgtaacat aacagaacct
     2881 ctatcaaaag ctaccattgg cattccatat actatacaag aattttcatc ctgtccaatt
     2941 gtaaatgaac cattttcttt catttttaaa agtccacttg ctccatcata acccatacct
     3001 gtaagtatta ttcctattga atttttacct gctacttctg ctactgaatc aaacaaaacg
     3061 tctactgatg gacagtgacc acttattctt tctccttttt cacaagttac ataatatcct
     3121 tgtttatccg tacacaattt catctggtaa cccccaggtg caattaacac atttccttgt
     3181 tctactctat cattattctt agcttctctt acattcataa tacactgctt gtcaagccgt
     3241 tgtgcataca tataagtaaa tatagctggc atatgttgaa ctacaacaat ccctggtgtt
     3301 gattttggta ggtcttttat aatttcaagt attgcctctg taccacctac agatgcgcct
     3361 atagcaataa ccatattatt aaaacgttcc acattaccgc ctccaaaaaa tcatatatca
     3421 aaaatacctt tttatcgttt atatatagca ggttttacat atttgtattt agtactactt
     3481 ctgttgatta cttctgcatg tcctacaaac aaaaaactat cttttttggt agcattataa
     3541 aatttattta taagttcttc tcttgttttt aaatcaaaat aaatcataac atttctacaa
     3601 aaaatcaaat caaaatgttg atatgagaaa tcattcatta aattaaacgg cttaaaaatt
     3661 acactttttc taatcttatc acaaatttta aatgtaccat ccttgttgtc tataaaatac
     3721 ttcttcttcc ataactcagg aacattgtta acatttgatt ctatgtatat acctctttta
     3781 gctttttcaa gtgagttagt tgaaatatct gtggctatga ttttaatgtt ccacaactct
     3841 ttattatatc caaaataatc atccaaagtc atagctattg aatacgcttc ttctccacta
     3901 gaacatcctg cactccatat atttatgttt cttattttat tattttcttc ccaaaaagga
     3961 agtatactat tctgtataaa ctcaaaatgt tgaggttctc tcataaaaaa tgtgtggtta
     4021 gtactcaatc tatttattag gtttatagct tcatttcctg tcctatcttt aaataataga
     4081 ttcatatatt gactaaaaga actaagtttt ttctctatca ttgtattgta taagcgacct
     4141 tcaattaacg ctcttttttt acttaaatca ataccatatt ctttttttac atggtttaca
     4201 agaactataa attcttcatc tgtgagtttt atcatgcaaa aacttcctcc tgctaattta
     4261 tcagaactaa ctatatgcta ttaatcaaaa actttcgaat atctaaaatt aatatagaat
     4321 tatcttcaac tttcgcaatc tcttttatat ctttattaat attagtgttg ttagaattag
     4381 tcgtctctag taaatttaga ttttctatat gaataacatc aacaacttta tctacaataa
     4441 ttccaacttg atactcatca acctttgtaa ttattataca agttctctca ttataatcag
     4501 ctttaggaag attgtacttt atatgcatat taataactgg tattattctt cctctcaaat
     4561 taataactcc acttatatat gttggaagac atggtacata cgttattgat tgaaatctta
     4621 tggtttcaat cacattttct aattctattc cataaataac atcattgaca taaaatgtta
     4681 atacttgttt aataatttca tctttagcca tactttaaat cccctaatat atttctataa
     4741 tgctatctgc atctataatt aaacttatac ttccatcccc aagtattgta catcccgata
     4801 aacccttatt ttttatatta aatcggttaa aataaactgg aaatggtttc acaacaactt
     4861 gctgattacc tattatttca tcaacaaaaa tgcatactgc tttatgctca ttttcaataa
     4921 ttatcatatt accattcatc agattggtta cttttgtttg aatattatat attttatgta
     4981 accttataat gggaatacat gctccatata ttgtaattaa ctctgtctta tttccttctg
     5041 taattatttg atttaagtct gtaagtttaa atgttcttct tatggatgat attggtaaag
     5101 taaatagtga atctccaact ttaaacttca taccctctac tatagaaaga gttaatggta
     5161 tttttatagt aaaggttgtc ccctcatcct gtttactttt tacttcaatt gaacctccaa
     5221 ctttttcaat acttttacga acaatgtcca tgcctactcc tcttccagaa aattcagtta
     5281 tagattcttt tgtagaaaag cctggagtca taatcaaaga attgacctcg tcatcagtat
     5341 actcattact ttgatgttta agaagacctt gttccttagc ttttgccaaa atcttatctc
     5401 tcataatgcc tctaccatca tctgaaatac ttatcacagc ttctccgcct agattttcag
     5461 cagataaaac tatctttccc acttcttctt tacctttcgc tatacgctcc tcaataggct
     5521 ctattgcatg gtccatagca tttcttataa ggtgcataaa aggttccgct aaattatcaa
     5581 ttacactctt atccacctct gtttctgctc ctatagttat taactctaca tttttatcta
     5641 attttttatt catatctcgt acaattctat gcattttttg aaatacacca gcaatttgaa
     5701 tcatacgcat agacattact atatcttgca attcatctgt aagcttgtgt aattgtcttg
     5761 ttgccttatt gaaattatct atatcaacac ctgtagaatt tacgctattt acaaccattg
     5821 attccataat tacaatttct ccaactatat tttgaagtaa gtccaatttt tctaaattta
     5881 cactcataat actctgctta cttattggta aatgttcatt ttcttcactt agactatctt
     5941 tttccatatc agttttttca tttaaaacat cttcttgtat ctcatcttct gtatagttat
     6001 tttgataact tttacttact ttattattat cttttaattt attttctact gcatcattgt
     6061 ttgatgtact tttcatttct atttcttttt tactaatatt tatgtattct tttacataga
     6121 gattatcttc tatagtttta taaactattg ccttatcctc tttagctaca aattgtatat
     6181 aaaacccata acttttaata tactctgtag actctgcatt tgtttctaca tcttttggat
     6241 aaaaatctat aaattcacag taatctctaa gttcaaatac aagcatgaaa gctctcacat
     6301 tttccatcat gcaaccttgt tcaaagatta tttttactac atcagagtct tctttgtatt
     6361 caaaatttga atcgattact tctttttcgt ctttactttc taaatttgca ttatcctttg
     6421 atttcataga acttgataat tccaaagtac tcacatactc ttcaattcct tcaataaaat
     6481 tagagtaatc agaagatata caacctcttg attgtaactc atctatatct cttcttataa
     6541 aatctaatga ttgaaagact aaatcaaata ataggtcatt gtctaaaact tctattttac
     6601 cttcacgtat agcataaaac aagtcttcta cactatgtgc taaagcagat atattgtcta
     6661 accccatcat agatgctgaa ccttttatcg tatgcattat tctaaaagtt tcattaatgt
     6721 tgttatgagt aaatactttc tctttttcta tttgaatcat aatttcatct aattgttcga
     6781 gtaataaact tgtctcaaat ataaacatat ctaacatttg tctcatactc ggttcaatat
     6841 aattcacatt tatcacctac gacctacaaa taattttcta ttatatatgc atctcataat
     6901 ttaaatcctt gatgattctc ttatctcact atatgtacct cataatttaa atacacaatt
     6961 attctcttac ttcaatatat atacatctca taatttaaat ccttgataat tctcttattt
     7021 caatatatat acatctcata atttaaatac atgataattc tcttacttca atattcacct
     7081 cataatttaa acccatgatg attctcttac ttcactttcc tttagatgaa atttttctac
     7141 taattctttt aaaatttgag cctgagcaga catctcttct gaggctgctg cactttcctc
     7201 tgctgttgct gagttcattt ggactactat ggaaatttgc tctattccag ataaaatctg
     7261 tgtaattgct acttcttgac tatgtacagc cgaagaaata ccattaatat aattagtagt
     7321 tttttctaca ccatttacta ccaagtccaa tgcatttgca gtctctttag caattctgac
     7381 accattttct actgttttaa tagcttcttc tatgagaata gctgtatttt gagctgcttt
     7441 tgcactttta ccagccaaag acctaacttc atctgctaca actgcaaatc ctctaccata
     7501 ttgacctgct cttgctgctt caacagctgc atttaatgca agaatattag tttggaatgc
     7561 tatctcatct attgttttga ttatttttga tatttcactc gatttttcac ttatttcacc
     7621 cattgatgtc atcatatcat ccatacatct acttccaaaa ccaacttgtg atgcagattg
     7681 gtcagatacc tcacatgcaa gactagcact atctgcacac ttcttaattc catctgatat
     7741 ctcattaatc gtggaagcta attcttctac tgaactagct tgttcagtag caccctgtga
     7801 aagagcttgt gcacctgctg caacttgttc tgaagcactt gcaacttgct ctgctgataa
     7861 atttatttca gaaaaatcac cattaaaaga gtttattata ctttctaaag atattttaat
     7921 tgatttaaaa tcacctataa attcttggtc tatctctact cttaaatctc cttttgcaat
     7981 actagttaat gtactgtcta taaggtctat gtaatttaaa agcatacttt gcatcttttt
     8041 gacagaactc actaatagcc ctatttcaga tttactttct tcaacgtcta tacttgttct
     8101 taagtcccca gatgcaattc ttgaaaagtg tttttctact ttattaatag gaatcactat
     8161 ttttaatttt attactgtat attctattat tgtcaatatt attaaaaggg aaagtataaa
     8221 tattgttctt aatattagac tttgtaattc tttttttact tctacaattt tggaatttga
     8281 acgggtaact atatcctctt gaaattcatt tattaaagaa tccattttct cttgggagtt
     8341 tatatagtca ctaccaaaaa ttatactttg agctttactc ttatcattac tttcgactgc
     8401 ctttattgca ctttgttctg ttggtactaa agaatctgat atttcttttg cttctaagat
     8461 tttatttgac tcgctctttg taagaccaat tttttctaat ttgtttaaag ctgattctct
     8521 tgtttttccg tataattctt tttcataaat atctttattc tttttttctc ctgtaaatac
     8581 atatcttctt aatacatcac ttatctctct agttgtatct tttatatact gactttgagt
     8641 cctcaaatta ttcatatcat taatactttt ttctactttt tcaaatgctt tataagatga
     8701 aatagaagaa aaaatccctg ctagaacagc caatactata ataatcagca atacaattgc
     8761 taatttagaa agctttactt ctcttttcat atctgtactc cttcataaag taagtttata
     8821 tcttttttaa tcctttcatt gtactcacta cgctacaatc ttttcctaag tttttaccct
     8881 catacattgc tttatctgct aaggatatgt tttcttctaa tgatatattt gagtttactt
     8941 ctactactcc taatgtaata gtgcacttta ttttatgttc tttatagttg aaatcaaact
     9001 cctttatctc tttacggatt ctttctgaaa gtatcttagc accttctatt ggagtatttg
     9061 tgagcattat caaaaactct tctccacccc atcttgatac agtatcaatt tgtctacaat
     9121 tcttttttat tatacttgat attactgtta acacgaaatc ccctgcttca tgcccataag
     9181 tatcattgat aaccttaaag tcatcaatat caccaaggat aagggatatt gatgtttcac
     9241 tatatttatt aattcttctt tgctccataa gctgattaat tatatgtcgc ctattgtaaa
     9301 gacctgtaag ataatctctt acaacgagtc tattcaattt ttcaagagaa gattccagtt
     9361 cattatttat cttttttaat ttatcttgag atttctttac ttcaagatga gctataacac
     9421 gagcttttaa ttctgtcata ctaaaaggtt tcaccatata gtcaattgct cctacaccaa
     9481 atccttttac tatgtctgta tctgtatttt ttgaagttat aaaaattata ggtatatctg
     9541 ctgtacgagc attagatttt acttccttac aaaactcaaa accagagtta tctggtaaaa
     9601 ttatatcaag caaaataatg tctggtataa attcttcaat aaattctttt gcctctattg
     9661 cattaaatgc ttttttaacc ttaaaattat cttttgtaag tgttttatct attaagtcac
     9721 acatcagaac actatcatca acgactaaaa ttgttccctg ttgcttattt ttcatataca
     9781 aacctctctt aaacataata tattcaaatt ttaatgattt aaaattttta tacttaataa
     9841 ttataccact atattcttaa atgttctcat cacaatcttt ttataataac actccaacta
     9901 catccctatg ttacatgata gtgcaatcaa acccattgat taattcatct attaaaactt
     9961 catctttatc tgtcaaaagt ttctccaaat ctagtaaagt aattatttta ccttctaatt
    10021 ctccaactcc acttacaaaa taatccgaat attctatagt aactcttggt ggaggtgata
    10081 ttttactttt ttctaagctt actatatcat caactctatc aaccacaaat ccaactacca
    10141 agtcctttat ttcagttact actatataca tgcaatttgt agtcattaat tcatcttttt
    10201 taagtctcaa acgcatatct attatcggaa tgatttttcc tcttagattt attatgcctt
    10261 tagaatatgt aggtagttct ggaataaata taatttcttg atttccagta atttctatta
    10321 ccttttcaag ttcaacccca aaatcttgct catctacttt aaactttaga tacttaattc
    10381 cttcatcaat tagtccataa tctaaatatg ggtcatctgg aaatctcatt taatacctcc
    10441 ctcctaaaat acactttcta tgaaatatat attataattt cttcttacct ttatttattg
    10501 aatctacaag taattcaccc gtctcagtgt aaaaacgtat tgttctacca taattcatac
    10561 ctgtgtcctc agctaatatt ggaatattta atcctaaaag agtttttttt actgcctcaa
    10621 cattcctatg tcctatactt gcaattactg aattgctact tcctaaaaac atctgagcac
    10681 ctccagcaat ttttgctttt atatatattc tatttgcacc tatattctcc atagatttaa
    10741 tgagctcatt tattcctgtg tcagcaaatt tacacttatt ttcaatgtta atagcatctt
    10801 tactatatgg caacataacg tgtaacattc caccaattcc agctacttta tctatcaaac
    10861 atactcctac acaagaacca agtgcataag taactaaaac atttggtcta taggctatct
    10921 tcatatctgc aatgttcact actatctctc tattcatact tctaatccca acctcacgag
    10981 tatttctcta agtgagctca tttctggaat catcaaaatg tgactagtaa aataattatc
    11041 actcatttta aatttatttt ctataaataa gactttgtca ccaacatctg aaaatcttat
    11101 cataggtaca tttagaacag ctccaaccat atcaatacat atatctgaaa ttgaaacact
    11161 tatagtcatg tttaacatag atgcaatagc atttacatat gaacttgcca taatatttcc
    11221 tatttcctta attgctgaaa tttcaattgc ctcaatctca tctaaaaaag cttcttcttt
    11281 attaaaaaga gaacttagca taagttgagt tattgattca tccaacaaaa acataaacat
    11341 tccattgata tcacctttca tatcgagtaa aatacatact actttgtttt ctggaccacc
    11401 aagcaaattt gtcgcttcat tatattcaag tatcttgacc ataggtatta atacttctac
    11461 attgctattt atcatggatg caagtgcagt aattgcattc ccagagccta tattaccaat
    11521 ttctctaaga gcatctatat gaacatcatc aagttctaaa taatttataa tataaacccc
    11581 tccctttatt attgcattta tttgcttttc attgtatcag tgtatattta cacttttatt
    11641 ttaaagcatt atctacaact ttaactaatg tttctgtttt gaagggcttc actataaaat
    11701 ctaacgctcc aagttttatg gcttctacaa tcatattctc ttgtcctatt gcagagcaca
    11761 ttacaacctt agcacttgaa tccaactttt ttatttctct tagcacctct aaaccatctt
    11821 tttttggcat tgtaatatca agcaacatca aatcaggctt ttcatattta aatatattac
    11881 acgcttcttc tccatcattt gcttctatta aattattata gccactttta ctcagagctt
    11941 ctttaattat cattctcata aaaatagcat catctactat caaaatttta ttttccataa
    12001 aactaactcc tccatatgta caacaataaa cgcttttatc ttcagttatt gtctatacta
    12061 tctgactatt tttcgctaca caataaatac atttcattga taaataaaag taattattta
    12121 tgccatatta tacttaattc tcttaacata atttgtcaac aaaaaaactt atatatctat
    12181 tgtaatatat gtttggcgtt ttgacgagtt attaaaaata taattgtata aaacataaaa
    12241 agttatcaaa agtaattaaa ttataataaa acaactttat acctttttta tgtttgtata
    12301 aaaaaaattt tcagattcat taaaatcatt tctgtagatt catataggat ttgactttct
    12361 caatatctaa tagatagttg tttatttact taattatatt tctctatatg tattatcgat
    12421 taaattagat ttagctttat acattatttt ttatattttt caaattgtat actcataaat
    12481 ccagaagtat tctaatgtgt ttataatact ttttttcata ttttaaatta aataacaaat
    12541 cattatattt acaagtatca tttatcaata aacaatatta aatgaagaat atgtctgaag
    12601 attcaaagtg atactgatac tattaattac taaaaaagca gtaaatcttt tacagattta
    12661 ctgcttatag ttataaataa taaaactatc tataataaat gtactatact caatacttca
    12721 ttcaatatag taataacaaa tctactttat tcctatgtaa atatgaactt cttgtttctt
    12781 tgtatcaaaa gtattacatt gatactcttc aaaatcactt atataagtcc tatcaaaatc
    12841 catttgccaa aacttaaacc aaaaatctcc tacttctttt tgacctccta ctataacaaa
    12901 ctttgcatac ttacctgctg gtataacttt actaataata ctttctccaa cagtattatt
    12961 agtgtttaat tcttctaaat tcttctcttt atcagaatta ctatttactt cacaacatgc
    13021 aacaaagcta tacggacttg taaaatcacc ctggtaatct gtatatagac ctattgtctt
    13081 gtcattcttt ttacccttta tagcattata tatccctttg cccataaatt ttttccacaa
    13141 ttcacctata tcatttaccg cttgtccatc tttatttgta gtctccttcg atattcctat
    13201 aactattttc tcatttatct ctactatttc ataattcata ctttatacct cactttgttt
    13261 ttattaatat attttatttt gaactaattc aattataatt tataatatac gacaccttta
    13321 tgtcatattt ttaatataat taaactcagt gagatattat atactaaatg caagatatta
    13381 tatatttaat aaaatcttta atctagcatt atattataat gaataaattt tagacaactc
    13441 ccttagtcta gttattacat tatccctaac atctttaggt tcaagtactt caacattgtc
    13501 accaaacgat aaaatatatc cataaatcca ttcaccaata ggaaattctg taattacatc
    13561 atatgtatca tcttcatttt tagttattaa atccttatta aaatcatcaa atactctaaa
    13621 aagcatcttt tctttaaacc tcaatttcaa cgttatagta ttttccttaa ttactttaga
    13681 atcattcaaa catacttctt ctattatctt tcttgaaaac acttcatcct ttatagataa
    13741 attccttatc ctactaattc taaatatcct aaaatcatct tttagtttac aaaatccata
    13801 caagtaccaa gtcttactct tatacataag cttcagtggt tcgacagttc tgtttgtctg
    13861 gcttgacaaa ctatttacat aattaaaatt tatagtatta cgccttaaaa tagcttcttt
    13921 tatttttgta aatttatcat cttcattaaa attacttccc cattcagtaa agtctatttc
    13981 aatccagttt gaaaaattct gctctccaaa tattgagcca attttattta ctactgaatt
    14041 tatttctgga tacttagtcg cctgtaatgt tttcagtgca actattaaac tctctttatc
    14101 tttttttgac aatatagcct tgtctataga atattcttct attaaagata tccctcctcc
    14161 tttgcctttc gtcatatata ctggtactcc cgacatagac agagtttcta tatctctata
    14221 tatggttctt gtagaaactt caaacctatc tgccaactct ttagctgtta ctaatttttt
    14281 atttaataga attactatta tttctgttaa tctatttatt ttcattcatt accacctact
    14341 aaaattatta aatttattta atctaaatta atcatactaa tttttaaata aacaaaatct
    14401 tagaatctta tctaaattat tcttacatga ttttatttac tttctattat ctttctcact
    14461 ttggatatta tcatcttaat ttatttatta ctatatcaat ttacttatta tcatctcatt
    14521 tgttatcgtc ttacttaatt tacttattgc tatatcaatt ccacttatta tcatcttatt
    14581 taacttactt atttctatat caattcactt attattacct cacttgttat catcttactt
    14641 aatttactta tttctatatc aatttactta ttatcatctc atttgttatc gtcttactta
    14701 atttacttat tgctatgtca attcatttat tattatctca cttgttatcg tcttacttaa
    14761 tttacttatt tctatatcaa ttcacttatt atcatcttaa ttcatttgat tattatcatt
    14821 tactttattc aatttctata tcaaaaatta gtatttgtac ttaatactta aaattaattt
    14881 aaagtcttta catattattt attttttatt gtaatattca tttccatact ttactaagct
    14941 acttccactc atttatttta aaatttcaat ttactatttc tcaattctat attattattt
    15001 ttcaatttta tattactaat tttaaagctt tttagctatt attcctacaa tatttaaaca
    15061 attttttcat aatgaagcta tattacaata tattcaaaat atgtaatatt ttagatattt
    15121 ttcgttgaca atgttaaatt tttgttttat ccttaattaa ggacatgtta ttgacatttc
    15181 tgaaacttat ttgaaatagt tttttcattt tatttaggaa aacgtattat acttaagaaa
    15241 acgtattttc tcatttcaga taagtaaaat atttattctt ttgaggagga aatcatggaa
    15301 attaataaaa aacattttat tcaagtattt ttaacttgct taatcttggt agtaatatgt
    15361 gaatttatag ggcaaagaac tatagctatt ggaaaagcct ctatagtact gttccctatg
    15421 ctttatgcag ttataattgg acttataata acaccagatt tattaggtaa aaaaattaaa
    15481 gcacttaaaa aagtggttgg agaaaaagaa ataaatattg ctggtgaagt tgttggaatt
    15541 gcactagtaa tgcttggtat aaaatatggt actacagttg gaccaaacat tgacaaaata
    15601 attcaagctg gaccagcgtt tatcgctcaa gaatttggtc atatacttgc accaatcgtt
    15661 gcattacctc ttgcattaat gtttggaatg aaaagagaag ctgttggagc tactgcaagt
    15721 ataagtcgtg aaccttcttt aggagttata tcagaaaaat atgggataaa ctcaccagaa
    15781 ggtagtggtg tacttggtac atatttaatg ggaacagttc ttggtactat atactttagt
    15841 gtactaggtt caatatctat ttatagtggt cttcacccat atgctttggg aatggcctgt
    15901 ggtgttggta gtggaagtat gatgacagca gctgctggtt cattatctac catggtacca
    15961 cctgaaatgg cagatacaat acttgcctat gctgcaacaa gcaatatgat gtctagtata
    16021 acaggaatta tattcttagt ttttgtatcc ttaccattta ctaattttat gtataagatt
    16081 ttagaaccta aattttcgag aaataaaaaa actaaagaaa ttaaatctac agaaggagag
    16141 tgctaataaa tgtttgctaa tttaaattta aagaaaagtt catatcaatt attggttatg
    16201 tctgttattg gagttataat tcttatagga caaagaatta gcgttggtac atctatagtt
    16261 acagctcttc ctggtatggt gatgctaata ttagctgcta tggcagctat gattataaaa
    16321 gatttatttc ctaaatcaat ctttccagct tttggatttg caactataat cggcctttta
    16381 cttagtatgc catatagtcc aacatctgaa gtattcttaa ctaacactaa taatattaac
    16441 tttatggcta taactactcc actactagct tttgcaggaa tttctgttgg aaacaaaata
    16501 gaagctttaa aagaaatgtc atggaaaata gttataattt cacttattgt atttacaact
    16561 atattctttg catgtgcgag tatagctcat atagttttaa gtattcaagg taaaatatag
    16621 gaggaattta ttatgaataa agaacaatta aaatctctag taataaaaac tatagatgaa
    16681 aatagagaaa aaatattaaa aataggtcaa ggaatataca aaaatccaga atatggatat
    16741 aaagaattta aaactacaga agctgtttca aacttttttg agcaagaatt aaatttatct
    16801 gtagaaaaaa atatagcagt tacaggatgc aaagcagatg caaattctag caagaaaggt
    16861 cctcatatat ctatacttgg agaattggat ggtatatcat gtaaagaaca taaagatgct
    16921 aatgaaatag gtgcttctca tacttgtgga cataatattc aaatagctgg aatgttaggt
    16981 gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt tagatggtaa agtatctttt
    17041 atggctactc ctgctgaaga gtttattgag ttggaataca gagagcaatt aaaaaaagaa
    17101 ggaaaaataa cttattttgg tggaaaacaa gaattaataa aaagaggtta ttttgatgat
    17161 atagacatat ctatgatgtt tcatgcttct gatttaggag aagataaggc tctagttggt
    17221 cctgagagta atggatttgt tggaaaaaag gttaaattta taggtaaaga atctcatgct
    17281 ggttctgctc cacatgatgg aataaatgct ttaaatgcag caatgcttgc tataaacaac
    17341 gtgaatgcac ttagagaaac ttttaaagag tctgaaagag ttagattcca ccctatcatc
    17401 acaaaaggtg gagatatagt aaatgttgtt cctgctgatg ttcatatgga atcatatgtt
    17461 agagctagaa ctataaatgg tatgattgat gctaatgaaa gaataaataa ggctttgatg
    17521 gctggtggaa tggcagttgg agctgatgtt gagataactg aaattcctgg atatcttcca
    17581 atattacgat acaaaagtct tgataattta tttaaaaaca atcttgaaga tttaggtcta
    17641 aaaggaaaag ttattgatgg tggagatttt acaggctctt ttgattttgg agatgtgtct
    17701 catataatgc ctactcttca ccctatgatt ggtggtgtaa aaggagctct tcatacaaga
    17761 gattttgaaa tagttgatga agatttagct tacataattc ctgctaaagc aatggcactt
    17821 acagttgtag accttctttt tgataatgct aaggatgcta aagatatatt agataccttt
    17881 actccagtta tgactaaaga agaatatcta acattcttag aaaaacacga taaaacttta
    17941 taagtctatt tttataatag ggtggtatct actgtaaaag ttataccact ctttgttttt
    18001 gtaacagtat acttcttttc ttttagtttt tagattaaac taaaatatat tatcctcctt
    18061 aaaactttta gagctacgtt gaaatatcag tagaactatg ctatattctt tctaattaat
    18121 ataaatttct attctaattc aaggtggtga aaaaatgttt aatgcaggaa atcgaattat
    18181 aaatacatgg ttgtacccta ttcaagatgg ctttgtcttg attgatactg gatatgaaaa
    18241 tggttttaaa catttcaaaa aacgaatttc taaattcaac ataaaaatca aagatattcg
    18301 atacatattt ctaactcatg ctcatgatga ccatgttggt tttctaaatc agattctaga
    18361 cgaatctcct aatacaaaga ttgttatgca tgataaagct ttagaaattc tatataaagg
    18421 tcaaaactct tttattggtg gttgtaccag ccgtactgcc tttctctttt gccaaattat
    18481 gaaactattt ggaaaaggtg agcatagttt ttctccactt aaacaagaat ttgaaaaaag
    18541 gtgtattctt gtgtctgaca agaatcgtaa agaaattgaa aaagaactaa atggaaaaat
    18601 tatagataca ccaggacata ctgatgattc aatttcacta ctaattgatg aaggtgtttt
    18661 attttgtgga gattctgcaa tgaatggatt tccaagtact cacaaaatta ccatatttgc
    18721 agaaagcaaa ctagaattta ttcaatcttg gaaaacaatc atcaatacaa aacctaaaat
    18781 gatttatcct ggacatggaa aaccatttga atacagtaaa ttagaacaaa atctaatcta
    18841 tgtaaataaa ataaatttat atcctctaca taattcaaaa gatattcaca gatttttgta
    18901 gaaaatacaa agtaaattta tttgattaat taaaaaatct aacactttac tacgctactt
    18961 attttaataa cttaatcttc cactagcttt tcataaaatt ttgttttaaa attaatttta
    19021 aagtgcatat gttatttaaa tacattagat aaaattcgtc attaatctac ttaaagcata
    19081 tttaactaac accactcaac cagaaaattc agaaaactgc attgacacag gaaaaagttt
    19141 attttatact ttttttagga catattgtgg acacgtctta aatcgacaaa cagaatttaa
    19201 attgtaagat taaccacaat acttattttt taattatatt tttaaacatt agggaggata
    19261 ttatgaatga taacgtagtt aagaagaaaa acttttttga caggacatta aacagaatag
    19321 aaactgttgg taataaacta ccagacccag ttacaatatt tttagggtta tgtgtacttt
    19381 tacttatact atctagtcta gttggttcta tgggtatatc agtagttcat cctggtactg
    19441 gagaaactat aaccgctgta aatttattga ctgtagaaca gcttcaaatt cttttaggaa
    19501 acatagtcag caatttccaa gggtttgctc ctttgggtct agttctagta actatgatag
    19561 gtgctggtgt atgtgacaaa acaggactta tgacagcaac aatcaaagca tcagtaagca
    19621 agattcctga aacaagagta actcttgttg taatgactat aggtatgctt gccaatatag
    19681 cttcagatgc aggtacaata ttatttccac cattagctgc acttgtatat cttggtgttg
    19741 gtagacatcc acttataggt ttattttctg gatatgcagc agtctgtcta ggatttgccg
    19801 caaacattat gataagtgtc aatgatatac ttgctgcaag ttttacagtt ccagcagctc
    19861 aaatgattga ttctggattt caagccaatg caacaatgaa cttatttttt atgatagcat
    19921 caacttttgt tctaatactt ttggcaacat gggtaacaga aaaaattgta gctcctagat
    19981 ttggtaaata tgaaggaaat gcagaactag atgttgatgg aagtcttagt gatgtagaaa
    20041 agaaagggct taaaaaagct agtataagta tattaatata tgtggctatt atagtagctc
    20101 ttagtgtaat cggtaaaaaa ccattcttag ctgacccaga aacaggagat ttactatcaa
    20161 acaatgctcc tcttatgaaa ggtatggtac ctataatagc actagcattt ttcatacctg
    20221 gtgttgtata cggaaaaact ataggtaaaa taaagaagga taaggatgta gtctctataa
    20281 tggctagtgc aatgagtgat atgggtggat atataatact agcatttgct gcttcacaat
    20341 ttttgcaatt attcacaaat agtaatttag gtctgatact agctgtaaag ggcggagaat
    20401 ttttaaaatc agcaggaata aatggacctt tattgttgat aggatttatt atattatcat
    20461 gttttataaa tttatttata ggtagtgcat ctgcaaagtg ggctattctt gcacctatat
    20521 ttgtaccaat gtttatgatg gttggataca atccagcact tactcagatg gcttatcgta
    20581 taggtgattg tataacaaat ccacttagcc cattatttcc gtacttccct attatactag
    20641 cattcacaag aaaatacgat aaagatgctg gtatgggtac tgtaattgct aatatgatac
    20701 catactctat atcattctta atagtgtgga ctatactatt aatattgttt atggtattta
    20761 atattccact aggacctgga atacttcctt catatagcat gtaatttatt taaatctttc
    20821 taaaaggaga aataatttat gttagataaa aatagactta taaataattt tatggatatg
    20881 gttagaattg atagcccatc taatcaagaa ttagaaatgt ctaagtggtt ggtaaactac
    20941 ttaaaagaaa gaaatataga cgccattata gatgatgcag gtaaaaaata tggtggtaat
    21001 acaggtaatg tcattgccta tataaaaggt gaagaaggaa gtagaccact ttgtttatgt
    21061 gcacacatgg accaagttca accttgttta ggtgtaaaac caatactaga tggtaatgta
    21121 gttagaagtg atggaactac tacacttggt gcagatgata aagctggaat agctgctata
    21181 cttgaagctt tagaacatgt tattacagaa aaaataccac atagagatat ttatctttgt
    21241 tttacaatct gtgaggaagc tggtatgcat ggcgtcaaaa actttaatcc tgacaacttg
    21301 ccttgtaaag atatggtgat tttagattct ggtggagcta ttggttcgat agcatacaaa
    21361 gctcctgctc aacaatctat aaaaatctct tttcatggaa agaaagcaca tgctggtata
    21421 gaacctgaaa aagggttaaa tgctatatta gtagcctctc atgcaataag caacatgcac
    21481 ataggcagaa tagattcatt gactacttca aatataggta aaatagaagg tggtggagct
    21541 acaaacatag taactgataa ggttacattg actgctgaaa ttaggtctca tatacctgaa
    21601 actttagagt atgagcttaa tcatatggaa aaatgttgta aggatgctgc atctaaattt
    21661 aatactactt atacttttga acataatatg tcttacccta gttttgaact tagtagagat
    21721 agtcacgttt tcaagttaag tgaagaagca ataagacaag ttggtgttgt tccaaatcca
    21781 atggtaattg gtggtggaag tgacgcaaat atattagcta atcttggata taattgtgct
    21841 atattaagtc taggtatgta tgatgtacac acagttaatg aatatgtaaa tatagacgaa
    21901 ttatatgata ctgccaaaat agtttatcat atgattaaat tataaatatc ataatagaac
    21961 tctttaaatt ataaaaataa aaacctatgt atcttcaact ttataagtta tatattagag
    22021 ttgttttaca taggttttag tatttttata ataatttgtt ctaatttttt tcataataag
    22081 ctttaatact ttatatagta atttgttgta gaattttata caatataatg atttatacag
    22141 ttttataaaa tatcttaaaa cagtatttca gtaatatttt ttggtcttcc tccaccaata
    22201 ctagtctctt ttgcacatat tcgacctaaa cctgcattta ctattttatt taatatacgt
    22261 cttgcacttc tttcacttac atttagatac tctgctaact cttttgaatc ataaatctta
    22321 ctttttctat tctcatttat agcaataatt tttgcaactg actcacaact cattccagtc
    22381 tttttagata tttcgattac cttttcatca gatagcatca aagaataatt taattcattt
    22441 tcggaagcta aaggtcctct tatgttttca tcttcatcta ccaaatatat accaatttct
    22501 tttgaatcta tggagtgtct taatgcttta tgtgcattat tttcagcctg atacgcagtt
    22561 actccagtac caattcctat actaagagaa aagcccatgt cttttatctc tctctttaga
    22621 ttgaatagct tatcataatt atactcatta tcagctaccc ctttatgaac aaatacaaca
    22681 taatcatttc ttccaagtaa aaataaggaa ccttgaatgc ttcttacata gtttactaca
    22741 agcttatcca tatctgattt ttttatcatg tcagaataat agttatctat cttctcttta
    22801 taatctatta aactcaatat ttcaacagca atttgagaaa atcttgcctt acttagagca
    22861 tattcactct ttaccttgtc ataactttct ttgattaatt gtatagtagc ttgtaaacga
    22921 aatacaggat atcctctttc ttttaattca ttatatactg caccaaaacc agtcaacatt
    22981 atatccgtct tattttcttc aaataatttt atatgccatt ctacaagttc agattctcct
    23041 acttccaaag aaaatggatt acaaaataca ctcgttgctt taatgtcaaa ttctttaagt
    23101 gcatcttcca caacatatct atcaactaca tctatactga atttatctaa ttttatattt
    23161 gcacttttta ttgcccaaaa tgccttcatt atactcgtcc cacttctcgg tacaaatgaa
    23221 tgtggcttac ttatctcata cttagatttt ataaattcat aaactgcaca tcctgtaaat
    23281 ataatagcat cacattcgtc ttcacattta gaaataactt ctattgtatc tactactttt
    23341 tctcttacat atattttagt ctttaaatca ctgtctatct gttttaaatc atcctttatc
    23401 ttaaacccag agtctgaagg acctataata cccactatca taaacttcgc tcctaatcca
    23461 taaatattat actttttaaa aattatatcc tcactatttt ctacttctat ttaattattt
    23521 tgaactactc catcctacct acatttagaa tggaataatt agcttatttt tgataaagta
    23581 acttctcaaa atcagcactt ggaataggct tacaataata ataaccttga attaccacac
    23641 aatctaaact cactaaaaat tcagcttgtt cttttgtctc tacaccttct gccactacat
    23701 ccatatctaa atcttttgcc atggctatag tgtgctttaa aactattttt tctctgtctg
    23761 tgattccttc ctcattaaag aaaccacaat ctatctttaa tgtatcaata ttaatatttt
    23821 ttaacatatt taatgaagaa tatgctgaac caaagtcatc catatttaaa acaataccat
    23881 ccattctaag gtcggctatt atcttagtaa ataattttat atttttaata aataaacttt
    23941 ctgtaagctc taattctaaa aaagattttg gtatactata tttatttatt aaacttaaaa
    24001 caatttcttt aaaatgtgga ttatgcaaat gtaatcttga tacatttaca gagataggaa
    24061 ctggctcttc acctaaatct atccattttc taatcaaaga acatacttgt tcccatacat
    24121 acatatctaa tctgataata aacccattct tctcaaatag tgaaataaat ttaattggag
    24181 gtattatacc tttttctgga tgaacccatc taaccaatgc ttctgcacca acaattttat
    24241 ttgcattaag attatactta ggttgtaaat atacatggaa ttgcccattt tcaagtgcgt
    24301 attccatttc cctttccatc tgtttttctt caagtgcatc atttcttata gtatcatcat
    24361 aaaatatata gttgttgata ctttgtttct ttactttttt gtgagcatat cctgccattt
    24421 cacacgcatt tgtaattgga atatgattat ccaaaattct ataaactcca aaacatggta
    24481 ctaacatata ttcaaatttt tttctattta agctattttt aatcttataa attaccttat
    24541 gaatatctct atcagagtaa ctctcataaa aaatgcaaaa tacatctgca tacatacgac
    24601 aacatatacc attagaacca atgttttttt gaatcaactc tgcaacatat attaaagctt
    24661 tatctcctcc tctaactcca taaatgtcat taattacctt gaacttatct atatccatca
    24721 ttataattgc ataacgcttg tcaggatatc ttttaagcat taaacttatt tgttcacaaa
    24781 aactttcata attgtatagt cctgttaatg tatctttttg gtttttatgt agttgcataa
    24841 gataatctat attttttata gcaactaaaa tatgtgtttg tttctcacct aacttatttt
    24901 tactactaat ctttaacata taaattaatg cccaatagta ctttttatct attcctaaac
    24961 gtctatactc aaaatttagt tctaaatcgt cttgcttgct attattaaga ctatcaagag
    25021 attttagaag gtggttatca ttaaataata ttatatcatc aggatgaatt aaatgttttt
    25081 ttatgtatga aaaagttttt tcaagatttt cctttatagg aaaactaaaa atattgctct
    25141 ctgattgata aattggagta attaaattcg tatcaatgca aatagataaa attatatcat
    25201 atgtattata agctaatttt tcataagcct tagaataccc ttttatcatt aaatcatcaa
    25261 aataaacctt atcaactaac tctttatgtt taaataaaat attattcgtc gaatcagcta
    25321 agtcactaat tactttcatt tcattatttc taagtttgtt cttattataa tacctcttct
    25381 tatcttggta cattctctca tcagcaactt taattagctt atgtatatca atattatcgt
    25441 cactccaaat actgcccact gctacactat atatcatctc atccttaaat tcttcactta
    25501 accaactcac taattcttca aatagttgtt ttgatatatc ttcacataat acaacaaact
    25561 catctcctcc aatacgataa acattataag aatgaaagta tttttttatt attacacatg
    25621 ctgacacaat agcagaatcg ccattagcat gtccaaagtt atcattaata tcttttaacc
    25681 catttatatc tacaaataca actcctatgg atttaacttt tcctttttga aaattatcta
    25741 tatacttaat atatttattt ctattatatg caccagtcaa gaagtcatag tagctcatat
    25801 aatttaattt attttctact tttctttttt tcatctcagt taataaaaaa taagataggg
    25861 atgtcaaaag agaggtatct ccaacagctt tatgtggatt atcaactcca ataaacccta
    25921 ctactttatc atcttgtttt agtgaagatg tcattaaact ttcaattttt tgaggtttta
    25981 aaatttcata ttctattggc tgttcttctt ttatattttc tatatcatca attattacaa
    26041 aacctttttg ttcgaataat aaaaaccaat tctttaattc attaatatca agatttgtta
    26101 gtgcctgttt ttgttcatca acaccttcat tgcaccattc atatgtatta gttacttttg
    26161 ttctatcttc tgatacttca aaaacatatg ctctatcagc tttatgaaat ttaccaatat
    26221 tggttaatac aagatttaca gcatcagaaa aattagattc tgttataaga tgtttaatac
    26281 atccaactaa taactccttt atctctaatt tttctgctat tgacttactt atatcttcct
    26341 tttctgtaat atcaagagct atttctaatc gtgcatgttt gccctcccat ttaattaact
    26401 tgtcctttaa tataaaatgt ctatttgttc gagtattcgt attttcccat atgtaatatt
    26461 catcttcttt taaaatagag tttgtacaaa attcgcaggg agaatctttc ccctgtaatg
    26521 ctttataaca ttttaaatgg gatatatcat taagtcccat tgcctttttc ccaatttcat
    26581 taatatataa aagttcatat gtatctaaag aagaaacata tacaatttca tccaactcat
    26641 ctattaaatt tattacatcc atttatatat atctcctttt attcattttc cagattgaat
    26701 ttttaaagct tttaaaattt tgctatatat ttggcaatga tattatactt ctttatgttt
    26761 taaaatagta ccttaatgag gttctatttt ttagtaaata gaattattat tgtgtcaatc
    26821 gtacaataga gttttgtaat acttaattta atatatttat aattaactat cttattatgt
    26881 atttacaatt aattctcata taaatgacaa atctccacct caattaagta ttccaatcat
    26941 atatgaggat aatacttcat tcatttaaaa atataaaact attatatatt aaaatttctt
    27001 caataataaa ttatttagat tgttactttg atatattata taatatcaca aaggctgata
    27061 taaaaagaca aaaagggcaa tttttttata tcaaaaaaaa tgttaaattg atattatcca
    27121 tatataaatt gatattatcc atatagatgc tatatttaac aattctactt acttccctta
    27181 cacttattag catatagtta ttaagagttt gttgcaaaac aacacttagc acactttaaa
    27241 aagtatggtc taataagtaa tcaacgaaga agacataata aagcataaaa tatgatatac
    27301 ttttccacgc catcaaaact gaggatttta ttattaatat atgctaaaat atgcttaata
    27361 aattaaattt taatgggaga atagttatga aaatatttag tttcttgttg tttgtgatag
    27421 gaatatgttt atgtattgct ggatttatag gacattaccc agtatttgtt ggtacaacat
    27481 tgagttttct tgcaattgca atgatagctg taagacaagt caaaactaag aaaaaaatga
    27541 aataggcaaa tcctaacttt acaagttaat attacacaag taattagtct aaaattggat
    27601 actttttcta ttacagaaat acatcatcca ctttgtattt tcttaaaaaa ataaaatagc
    27661 tatgtaaatt caagattaga tagattaact ctctatccat ttcattgtaa aatatacata
    27721 gctatttgta ttcgatttaa aatacttaga ttttcttaca taacaaataa tttctatctg
    27781 attattactc atattcattt agtaatcact cagcaactaa tcatttagta tctattcatc
    27841 cgccatacgg taacctaccc caacctcagt caaaatatat tctggctgag caggattctt
    27901 ttcaattttc cttcttatac ttgccataaa aactctaagc gactgatttt cactttcata
    27961 gtaatttccc caaactttat ctattataaa cttatgggtt aagactcttc ctgaatactt
    28021 agacatcaac tcaataatct tatattcgat tggagttaaa tgtatttctt caccattaat
    28081 tgacacaata tgattttcat aatctatctt taggttttta acttcaaaag actttggagc
    28141 atcattattt atattatcct gttgagcttt atttctaaga gccacacgca ctctagccaa
    28201 aagctctgtt acactaaaag gtttagttaa ataatcgtct gctcctccat ctaaagcttc
    28261 caccttatcc ctctcatgct ctctcgctga aacaacaatt attttattag tctttgcaca
    28321 tgccctaact cgtcctatta cctcaatccc atcaatatct ggtagtccta aatccaaaat
    28381 tatcaaatct gggtcaagag aatgtataag tgatatagct gttcctccat tatctgtctc
    28441 tacacaccta taattttgag tttcaagaga tacttttata aatttacaaa ttggcttatc
    28501 atcttctaca attagaacta atggctttat ttccacttgt ctttcccctc ctcatcatac
    28561 ataggtatgc taaatctaaa tgtagctcct ccagaaggat tattaaaggc gacaatttct
    28621 ccaccatgag cttctactat agatttacat atctccaatc caagaccaat acctcttctt
    28681 tcaagatttc tagacttatt cttagtataa aacctattaa aaatattccc gatatcttca
    28741 tttggtattc cttttccatt atctgcaact tcaaatagta cttgatttga ctcttttttt
    28801 acagttagtt ctatcttaga atttttaggt gtatgcctta tagcattatc aattagattt
    28861 actaacactt gttctattaa taatacatcc acatgaacga ttattatctc atccggaata
    28921 tttgttttta tgtctctact attggcaaac tttttgactc ttaatataga ttcagctatt
    28981 atttcatcaa caacctctgg tcgtttttca atatccaatt ttccttcatc tattctggtc
    29041 atactaagta tattctcaac agaatgagtt agccaactcg tatcttcata tatatttttc
    29101 aataattcta ctctagtttc tttatctatc acatcatcat tttcaagtat tgttgaagat
    29161 gaacctaata tacttgtaag tggagttctt aaatcatgag aaattgacct aagcaagttt
    29221 cctcttaatc tctctctctc tgcctcaaga tttgcttttt tatttttctc aaataaattt
    29281 tctctatcaa ttgccaacgc aacctgtgtt gaaactgctt ctaatagtat cttttcattt
    29341 tctgtaataa tatctatctt aaaacacgca acacctataa caccaagaac atttgattct
    29401 tgacctttta tagggaaata atatgcactg ctgtttttac aatattttgt cccaattcca
    29461 actgggttac ctgtcctaaa agcttctttt atagcttcct tttctaatac tgaatggaat
    29521 atattctcac ttccatcatt gttacaaata taaatacttg gcttacctag attattattc
    29581 tcatctgcaa tagtcactat tacagttcta tttaatatat ttactaaatt agacccacaa
    29641 aattcaataa tttgattttt attttttgct ttaagcaacg ttttgttatt ctcatagagc
    29701 aatcttatca ttttttctct cttatgagaa agtctcactt ctctttttat cctagaagtt
    29761 aaagtacttg ttataattgc tgccattagc ataatacaaa aggtcatagg ataatctttt
    29821 ctataagcta aaaaagtata taatggatgc gtaaagaaaa aattaaatga tagcactcct
    29881 attactgaag aaagtattcc aaaaaaatat ccataggtta tacttgtagc aaatagtaca
    29941 cctaatatgt atactagtat aatattagat tcattaaaac ctaccttttc aaaaactact
    30001 gatattccag tagcaattgt cataaccaat atagttttta tgaagtcctt aataattatc
    30061 tttttactga gatagccatc ttcatcataa tcatactctt ttaagtcttc atgttgtagt
    30121 cgttcctttt tcatttacta caattcctcc ttattgttac tgtttaaatt ggctctattt
    30181 tatataatac aattttatca taataaattc aatcaataca tttaattaat gtacttaaat
    30241 tactaaaagg tgctgtctta aaatgaaaat aaatctctat ttcaaaacag cacctatata
    30301 tttaaattac taatttagtt tttacatact taaatcaata gttttattca atttcataaa
    30361 ttataatact tcaattataa aacatttcta tccaacaagc ataaatataa agtgggcaca
    30421 tatacctgca cataaaccac ctatagtatg cgaaagtatc gctttaccag ccaatggtct
    30481 tgcatctaaa gcatccatca tacctatgtg tgtacttaaa tatccactcc aacacatacc
    30541 cattgctgtg aatactgcta tatcattagg agttatagcg cctgatttta taaactctgg
    30601 tactaaagat attgctgctc caacagctcc taatgcagtt acagggaatg ctattgcctc
    30661 tggtgatgtg aatccaaata atggttctat tatgaagcta atcttatcac caatagcagg
    30721 taataatttt ataccttcat atgcagctcc tgtgtaaaca gcttgacctg tagcagggtc
    30781 tgtagatggt ccaaaagtta ataacataac taatgtacaa actactaaaa ctcctggtat
    30841 tatagccata cccatttcaa cacctaactt accaccttca agtatagcat ccaatgttct
    30901 ttggaataca ttaccatctc ttatttctct aaactcttca agttttgcac ctttatcatt
    30961 tatttgcttt tcagtatcat ttttagggtc ataattataa tactttttag taaaagtcaa
    31021 cataattcta acactaataa tactacctat tattgccgca acattaccta ttatagctgg
    31081 tagtacatat tctttacctt gagctatcat aaaagttgtt acaataagtc ccataccaaa
    31141 ggctgttcct aaattacata aagcaggtac ttgatacttc ttaaaatatt gtgtaaatgt
    31201 tttgtcttta gcaaatggta taatagctgg attatcagat aaatatgttg caactactcc
    31261 tgctatacta gcaccaggta agccccatat aggtctcata agacccttaa atatcttatt
    31321 aactaaagat attactccaa actctgaaag caatgcacta agtgcacctg caagaactgc
    31381 catagccatt attaaaaata ctgtgtctaa taaaagcgca tgtgctgtac tcattattac
    31441 tttaaacatc attcctgcac ccatgatgct tcctacatat ccaaatccta ccgccaatag
    31501 tacaataaat acgaaggttt ccatacttac tgccttgact cttctcttat ccgacagctt
    31561 ttccatcttg aaatcttccc ccttttaatt ttaaattaag gttcctaaca ttggtacaaa
    31621 ttttttggta caaactgagt tctaaaactt acactcgtaa tgcaagttgt cagttttttg
    31681 aaataaaaaa aaattacgtt tatagaataa gaatatcctc tttaatttgg atactcttat
    31741 tctattttat gttattatat tatcatcctt ttgtttaaaa gtcaattttt tctatttttg
    31801 ttgtttaatt ttatttatac tctttttata tttttcattt tcttttatct ttgtaagtac
    31861 tccatataag ttgacttttc taaatacagt agttataaat accaatccta atgcaattga
    31921 acttgcacac gataatgtac tcaccccata ttctacagct tttatataat tattggtcaa
    31981 aacagaatac atagtattat atattccata accaggtact aatattatta aacagcaaac
    32041 acttaatgta gttgctggag tcatataaat tcttgcacat aactcacaat atatcgcaaa
    32101 acaaactgta gctataaaaa aacataagtt ttctgttact cccatattta aaagaaattt
    32161 atacgaaaac caagctatac ttccacctat tcctgctagt attaagtttt ttcctttcat
    32221 attaaatagc actccaaagc ttaaggcagt aaaaaaagct gctattactt ctataaacaa
    32281 atgcacttat atccccccta acttgaagta caatccaagt accaagaaca ttcctactgc
    32341 tatagataca cctacaaaaa atacttctgc taattttgca agaccagaaa tcaactgtcc
    32401 atctaacaaa tctcttaatg cattagtcaa agcaagcccg ggaactaaga gcattattgc
    32461 tcctgctatt aaatggtcta acgtctttat gaatccgagc tttaaaatta aaattgaaca
    32521 agaagctata ataactgctc caattgcatt tacaaaaaag tcattaaggg aagatttact
    32581 taaatacata ataacaaact tagtcagaat cccaacagta aatgcagcta taaattcttc
    32641 cacatttcct ccaaataata tcgaaaaagt tgctgctgca atacctgcaa aaaatattgt
    32701 tattaaatcc gagtaatagt tatcttcact tatttcatct aaaattttct cacataattc
    32761 aatatctaaa ttatttatac ttattgctct tgataatgag ttaacttcat gaaccaagtt
    32821 taggtctact cttcttgagc ttattctttt aattgctgaa tgtaatttcc catctacaaa
    32881 tacagatgcc attacagctg ttggagttgc aaatacatct acatggtcta ttccaaaaga
    32941 ttgacatatt ctagctatcg tttcttcaac tctataggtt tctcctccac tttgcaacat
    33001 agctttacct gcatttgagg aaaagtttaa aattctatct atatccacaa aacatatctc
    33061 ccattctttt atagtattat tcatatttta atacaagata ttatttattt aaactatttt
    33121 aaataattca tatccaatat ttacatttta tgaaaaaact tgttttttat gcttgtcaaa
    33181 atcaaatttt aagcttgttt acaccataaa atggctgaat taatatttga caaacacttt
    33241 caaccattta tataaccttc taaattgtat aactttttac tacaaataaa attatatctc
    33301 ttttaattct ttctcttgag tatttagctt atagtaactt catatttata tatgtacaat
    33361 ataaaacatt atttttatga tacaaaatat aatttgtaaa tgtatatatg tattaactgt
    33421 gtatatataa attattctta accttatcag ttttttcata tatcttcatc tataccgacc
    33481 atatattaat tacattgacc atatattaat aaatgatatt acatagactt aagtatatta
    33541 aaaaacttaa atttagttat ttaacaacat aatactctaa tttaatctat aaaaatccat
    33601 acctaatata atgcaactct tattttaaca ccatctatta cacaaaacaa catattaaaa
    33661 agtaaaatac aaaatatcca ctataaacct tattttaatg ctgaatatat attataatat
    33721 atttaatgta gcttaaatat agtttaaaca taaagaaagg aaacggtata tgctaagaaa
    33781 agataaagtt ctattatctc tacataaatt atgtgaaaat atgactttaa aagaccttca
    33841 atcagataaa acaggattca ctacaataga aattgctaac cattcatctc ttgatagaag
    33901 taataccagt aaagaattaa ataacctata caatgaaaaa aaagttctga aaatatctgg
    33961 taagccaatt ttatttctac atgctagtat tatagaaaat ttaattggcc gtactttgaa
    34021 cagtgatgaa ttttctataa ataattgtaa tgagctactt aaatcaagta aaaatgactc
    34081 agaagatatt ttttcaacat tacttggaca tgattctagt cttaaactac ctatagaaaa
    34141 agctaaatct gctatactct atcctccaaa aggtcttaat acgttactca taggacctac
    34201 tggtgttgga aaaagtacat tttccgaaac aatgtacaaa tatgctgtag aaagtaaaat
    34261 cttttcttct gaaagcaaat tcgtagtatt taactgtgct gaatatgcag aaaatgctaa
    34321 cttattatta agtaatttat ttggttatgt caaaggttct tttactggtg ctaataagga
    34381 taaattcggc atagttgcac aggctgatgg aggcatatta ttcttagatg aaatacacag
    34441 attaccacca gaaggtcaag agatgctttt tcttttgatg gacaaaggca tatacagacg
    34501 attaggagaa tctgacattg tgcataaagc taatgtcttt attgtgtgtg ccacaacaga
    34561 agatatacat tcttctctgc ttgggacttt tttaagaaga atccctgtta caatcaacct
    34621 acctgcttta caggataggt ctttaaaaga aagattatct cttattaaat acttctttat
    34681 tatagaatca aaatacacca atgcaactat aaaagttcac aaagatgtaa ttaaatcatt
    34741 actcctttat gattgttttg gtaatatagg tcagcttaaa tctgacatac aacttatgag
    34801 tgcaagagcc ttcttaaatt acaaaactgg acttaaggat aatatagaaa tagatttatc
    34861 ccttgttcct gattacatct atattggtct tttaaaattg aatgaaaatc gtagtaatct
    34921 aaataaatta tcagaacttg attatatgct tttttatgaa tttaatggta ataacttaga
    34981 agttgaatat acaaaagaac tatctaaata caatatagat acatcaaaca atggatttaa
    35041 atactctaca gagattcctt atctggaaaa tgatataagg agtatgttta taaattacat
    35101 ggaaaagtac tctacaaaat tactttcgat atcttcagta actcattctg attatacaag
    35161 taatgagatt tttaaagtaa tcaatccaaa gatttactat gctgttgaac attctttaaa
    35221 agaggctgaa aaaatatcga gaaacaaata cacaaaacaa acttatattg ctctttcaat
    35281 gcatataagt gcacttatag aaaatataaa tcaagacaca tataatccta agtacaattt
    35341 atataatgat aaaattttct tagataaaga tttaaatata gctaaaatag ttttaagtat
    35401 aattgaaaat gacttacaaa catctttacc taaagatgaa gtaaaattta tatccatgtt
    35461 tctaaatagc atgtatcttg aaccaaacaa tcaatctgaa agtataggag tcataatact
    35521 tgctcatgga aattcaacag caagcagtat gtgtgatgta gcaaattcat tgcttggtac
    35581 aaaccattgt aatgcaattg acatgcctct tgatgtaaaa gtaagtacca tacttgataa
    35641 aactatagag ttggtaaaag aatgtgacga aggtaagggt gtacttcttt taacagatat
    35701 gggctctctt atagcttttg gagaacttat ttcagaaaaa acaaaaatca aaactcgctc
    35761 aattgaaatg gtatctactc caatagtttt agaagcagta agaaaaagta cactaccaga
    35821 aatgactttg gatgaattag ctaattcctt aagctcttta aatccatata ttggacgagc
    35881 agcaatcgat tcaacagaaa taaactatcc tgaaaagtca agagagtatg taataataac
    35941 tacttgtata acaggatatg gttcagctat gaaagtagca gactttataa attcttcact
    36001 taaaaatatt agtaaataca atattaattt agttcctcat aatatagaaa gttttaaaat
    36061 gtataagaat aaatttagta atactaaaat tctggcagtt gttggaagtg tagattttaa
    36121 tgtagccaac acccctttta tacacataga ggaactacta aatggatatg gcctagatat
    36181 gctaaacaaa ataatagaag gtacattcaa tgccaatagt attacaaata taaaagaaac
    36241 aaatctatca tacaacagtt taataataaa ctttatgacc caaaacttag agcttttgga
    36301 ctctgttaaa ttattcaact atgtcaattc ttccttattt acaataatag aattaataga
    36361 tgaaaactta aagagcaaat ttaagctagg atatatacta cattgctctt ttatgataga
    36421 aagatgtttt aaaaatacaa cttttccata taataacatt actgaattaa ttagtaaaaa
    36481 tcatgaacta tatagtttaa tacgtgaagc ctttaagcca acagaagctt attttaatat
    36541 tttgatacca gacgaagaaa ttgcttatat aatagacatg attaatgagt atctaaataa
    36601 ataaaattta tagcacttat tttgtgcata ttaattaata tatgaaatgg aggacgagat
    36661 tttgaaagga aaaataaaac agttaacaat acttgcatta atttttatat ttataactcc
    36721 tgtatttgca tttgcagata ctccaccagt accaaattct tcaagagcag cactacttat
    36781 agaccaggaa actaaaagaa tattatttga aaaaaacatt gatgaaaaaa tgccattagc
    36841 gagtttaagt aaaatgatga cctttttatt ggcaatagaa gcagttgata aaaatcaagt
    36901 taaagagact gatatggtga aaatagacaa gtctacagct tcagtaggag gttctacttg
    36961 taaacttaaa gatggtgatg aaatatctct aggagagctt atgcagggtc taatgctagt
    37021 atctggaaat gatgctgcta tagccatagc taaacatatt ggtaaaactg aaaagaattt
    37081 tgtaaatatg atgaacaaaa aagcagaaga aataggaatg attgatacgt attattttaa
    37141 tccaaatggg cttcctatat atactgaccc agaacataaa gaacctccta ttgaaaatat
    37201 gtcaactgct cacgatatag ttacacttgg aaagtatatg tacgaccatt atgaaaatca
    37261 agttacaaga ataaccacta tgcaagtata taatgatact aaaaaggatt ttacacatta
    37321 taacacaaat cctctgcttg tttcagtacc tggtgttgat ggaataaaga caggatatac
    37381 agataatgct ggatattgcc ttgcattttc tatgatggta cctaaagatg ctaaaaacga
    37441 aagaaatcat agattgatag gtgttgtact tggtgatggt aacaagaaaa atagaatttc
    37501 ttcttccgct actttattga aatatggtaa agataatttc cattctaaaa agatagcaca
    37561 taagggtgat attatagaga ctccttgtgt tgatggtatt gatgatttta agataacagt
    37621 taaggttgat aaagatttgt atggtgttgt ttcagataac gaaaatatca atcctaaggt
    37681 agtgtttaaa aatatgaatt atcctatcca taagggagat attgttggtg ttgcaaagta
    37741 ttataatgac tctggtaaat ttgttggaag tgttgatgta aaaagtgaaa gtaatatagg
    37801 atgtattcct ttgaaggata agattaagat taaggttgct aagataaata aaaaacttga
    37861 gattaaaaat tctgtttgtt ttaaagcata gtaaagcata ttattaaaag ggtatgttaa
    37921 atttataaag actccacttt gtggagtctt tttgtttttt acaaaaaatt aaaatagtgg
    37981 caacaattga ggatgtgagg gagagttgcc actattttgt atagggtatt atattatgtt
    38041 tttaagctag ttttactatc ttctatgttt atataataac tcttgtgtgt gaatttaatt
    38101 gataattaat atgaaaatta tgtgaagttt tgatttattt gtaatatatt ctactactat
    38161 tataattttt ctactggata tactaatcat ttgtttaaca ttatctgaga ttgatttgca
    38221 aacttttata taactaaatt agttatataa cttatagatt ccattaaata ccctaataat
    38281 catatatgta catacaaagt catgcttatg caaaatctgc agtttcaact aacttattat
    38341 ataaaccatc tttatgtaga agttcagcat catttccaga ttctattact ctacctttat
    38401 ctaatacata tatattatca gcactcttaa ttgttgaaag tctatgagct attataataa
    38461 ctatcttatc ttgtatgtac tctttcaagt tgttcaatat ctctttttgt gaaatattat
    38521 ctagagctga agttgcttcg tccagaataa gtaccttttt attacttact aaagctctag
    38581 ctattgatat acgttgtttt tgacctcctg atattttgac acctttttct cctactactt
    38641 cgtccaatcc cttatctaga ttttttataa aatcatctaa acaagtaact tctaatatct
    38701 catctaattc tttatcactt attttattat ttaatactat attatttttt attgtgtcat
    38761 caaatacaaa ttgatcttga gttactatat caatattatt tcttagtgat tttaaattaa
    38821 tatcctttat attaacacca tctattaata tatcaccttc atttatatcc caaagtcggt
    38881 atattagatt tgttattgtt gatttgccac atccacttcc accaacaatt cctgttactt
    38941 cccctttctc aaattgtaaa tttatatcat ttaaaacaag gtttaaatcc tcaccatatc
    39001 taaaagatac atttttaaaa tctattttct taatttcact gttatctatt ctatatccat
    39061 tattatcaac tttaatatct atcttttcat ctattactga aaatactcta ttgatagaca
    39121 cttttgattg tgctattcta gtattcgccc ttactatagc tatacatggt ccaattaaca
    39181 ttcctgtata ttgttgaaat gctattaact cccccatcgt catcttatta tttattattt
    39241 tatatccccc aattccatat attaatattg ttatcaatcc tgttagtatt tgagatattg
    39301 ctatattact actcataatg atgttcaaat tcaaatcttt ttttatcaaa tctctctctc
    39361 tctttaaata cttattaaag aaattctttt ttgaatttaa tataacaata tccataatat
    39421 tagatacgta ctcttgaatc aaatttgata aactgcctga atcacttctt atttcagaaa
    39481 ttttcttaga tataagtttt gtcattttat tttgagaaaa tgttaatatc aactgtaaaa
    39541 ataaaactac taaaaataaa tcaaactgca aattaattaa gaaaattaac gatattatag
    39601 ctgtaaacac atttacaata atatcaaata taatctctgc tccaaagttt tctatcataa
    39661 atatatcact ttccataatg gataaaatat tacctgtttt cttacttgaa taatatctac
    39721 cagaaagttt tgatatatgg ttaagcaact ttacttttag atttatagta actttatttt
    39781 ttattgaagt atataaatat gataatataa catcaaatgt agctgataat atccttataa
    39841 aaatatatat caatattaaa tttataagca gaaaaatatt tttatttcct attccatcat
    39901 ctattataca tctaattagt tgaggtatta caatatttac gccagcaatt ataaaaacca
    39961 ttataaatga tactatcaac ttagtcttat tcttatatac tattatattt agtattctaa
    40021 ataaattatt attaatagtt tttttcatct atttttccat cttttatagt tataattcta
    40081 tcagctgtct ttgcaatatt atcattatga gttatcataa ctagtgtttg tttgtactct
    40141 ttaacacacc attcaagcat attcataact tcatttgtag tcttactatc taagttgcca
    40201 gtaggttcat ctgcaaatat tactgacggt ctattggcta aagctctagc tattgcaact
    40261 ctttgttgtt gacctcctga tagttcattc ggatatttct ttatttgttt ttcaatcccc
    40321 aacttattaa ttaatctcat aacatattct ttatcttctt ttttcccatc aagcaacaca
    40381 ggaagtacta tattatcata tacatttata actggtatta aattgtaact ttgaaatatg
    40441 aagccaaatt cctgtcttct tatttcagac agcttatcat catttaatgt atataaatct
    40501 acatcatcta agtacacatt ccctaaagtt ggcttgtcta agcctgcaac acagtgaagt
    40561 agtgtacttt taccagaacc acttgcacct acaatagctg taaatttatg tggctctata
    40621 tctaaatcaa taccatcaag agctctaact tcattctcat attttccata tatttttttt
    40681 aaatctttta ccttaagtat tttcataacg tatctccatt cccatttaaa aaacatatat
    40741 ttttaaatta aattttcgta aataagatct actgccatat ctaaattctc taattgatat
    40801 aagtatactt ctttgtagtc aatactaata ttaaactctt tatttaaaat agttaaaaaa
    40861 ttatatgcat tataatttcc aatacatgta aaattgttaa ataaagaata ttcacttaca
    40921 aattcaaagt ctatttttat tttagaaaag tttctattta tcacatttat tattttcctt
    40981 gctatatcca atcttatcaa attagattta taacttggag tgttcaagtt ttctattttt
    41041 aaatcttctt tacttttgct tttttgaata agtatttctt ccaattcatt aaacttaata
    41101 tatggattgt tttttaatat atttgcaaca atgccagttg ccactgcaca agccttacta
    41161 tttttaccaa aatgagttac tttatcttta aatttaataa aattatcttt tgaatttgca
    41221 tacatttgaa tcttattttc tggttcgtat atatattgat tatttttata tatattttca
    41281 ttacctacta caccaataac actttcaaat atagtaggaa tactttcttt atcttcatat
    41341 ttattatgtt ttgaacaaat aataaatcta ccttgactat ttaaaatatt acaaaccttt
    41401 tctaattctt tttcatacat actattaata gatgcagctg aaatgtttat tatctgtata
    41461 tccgacatag tcaacttgct cagtgtacta agaagattta aagaacttgt ttttccagaa
    41521 tcattaaata tattaatagg ataaatttgg gtatttttac ttatatttaa tatggttttc
    41581 gatacaaaag ttccatgtcc atttatgtca tttatttcct tcccccccaa atctttcaat
    41641 tgaaaatttg aatcataaac ataatttcca ttaatatcat catcatttac atctaatcct
    41701 gtgtctaaga ctgctattct tactttagtc aattaataat cacctctaaa ataaattacc
    41761 ttgaatataa aactaatcct taccataata taggttccaa gatgtttttc atttttttac
    41821 aatttgcatc aaacattttt ttgttatttt ttaaagtata tattttcgat ggacaagttg
    41881 tacaaaataa atttacttta cattctttac aactctcaat atttactggt tttaatttat
    41941 caaatatatt aaatatatcc atttctctat taagaatcct ctctaatatg ttttcattaa
    42001 attctaatat attgaatatc tttaaatcct tatactctaa gtttggacac ggatatacat
    42061 ctcctttatg atctatactt atctgattaa cacctgcttt acacatattt gaacataaac
    42121 tactttcatc cacataatca tctttgcaaa tgtaatcaat atcaagttta tcattaagat
    42181 atttgctata gttctcatta cctctaccaa tattcataaa atttcttatt ataggcgttg
    42241 tacctaaatc ttcatttaat ttattgaatt tctctatgtc tttctcatta ttctttccta
    42301 ccaccataga caaacctacg ttctcgaatt tttttagttt tataagtcta atcacattta
    42361 atactttaca gaatactcct ttacctctta cttcactaca acttaattca tcataaccat
    42421 ctaaactaat ttctattgca tataaaccat taattatatc atcaatctgt ttttctttaa
    42481 ttaaagttgc atttgtagac agaattattt tacctttaaa tttttctttt atataatcca
    42541 acagctctaa gaaatcattt ctcatcaatg gctctccacc tgtaatggtt aatatccttg
    42601 gatttaattt aactatatta tctattactt ttcttacttg agatgtatct aaataatcgt
    42661 tattatctat tttagaatct acacagcaat aatcacaact tagattacat ctattagttg
    42721 gagaaaatga aattttgttt attctctcta gtctaaattc acttagaata gaatcactta
    42781 gaagtcctat atgctctaac ttatctataa ctcgttcata atatttttta tcttcatttt
    42841 cttggaatac atcatacaaa ttaccaagtt gtaatgcatt atctactgaa tactttagaa
    42901 cttcataaca ttctattgga attttaaccc atcttccatt tactttatta ctaagaataa
    42961 cttgattatt atttattatt ttttcacata tacaatctaa aaaatatttg tttgagctat
    43021 tcatttattt tatttccttc ctatatattt aaattagaaa tttcattaat tttcttttat
    43081 accacttact atactaagtt tatttatcat tttattagaa aaataaactg caatgacaca
    43141 aacaaaaaca gatatagatc caaatattag aatttcatat attggaattt caaatttaat
    43201 agagttattt ataccatata cctgttgatt aagtttgtta gtttcagaaa ctattttata
    43261 ataattgaaa ctagatataa ttccagctac aatcatactt aataccccat atatcatatt
    43321 ttcttttatt aacattcttt ttacattttt tttggacatc cctattgctc taaatgtaca
    43381 tatctcactc aacctcacaa ttatatttga tctaactata aaaataatgt ttatagatga
    43441 tattaataaa actccaaata cacaaatcat tgctaattta tatgaggatt tattataatg
    43501 gtctccaaaa tgattacgta catatcttcc ctctatatct gaaaatccac ttttttctat
    43561 tatattattt atttctttta ttactttttt atctgtttct ttttttatct tcacaaaaac
    43621 tttattataa tcacttatcc ctgttaattc ttttaaatcc ttttcattaa atataatttg
    43681 aattccacct gattgagatt ctccatcttg ctctgatata tagctatttt tcattattcc
    43741 agcgattctt acttttgtat ttatatattt atactctcca tctttatata caggaatttt
    43801 tatatctatt aagtctccta ttttaggtga ttttataatc tgagtattat ttgacaattt
    43861 aattctagaa taaaaattat ttaccaataa aacattttta taatccctgt ctgtataact
    43921 ataaacattt tcgcctttat ctatatattt atctatatta tttaatagct ctttattgta
    43981 acctcgaatt aaaactgtag actcattatt attatttatt tcagttcttt ttagctcatc
    44041 taaataatct tcttctaagt cagcatttct tagatatcct tttaaataaa tattgggttc
    44101 aacatctgtt acatcttcaa tagcttttat ttcatttaat aaagaattat catatttaga
    44161 aaatgtataa tttgcattta ttggtgaata tcccaaataa atatcaaaat tgccatatga
    44221 tttacttgtt atattagctg aatctgactc ttttccatat atgaattttg tagaagttag
    44281 tctgcctata aataaaaccc cacatagtga tatcgtaatt attattatta ttgatgtaac
    44341 cttattccta aatatatttt taaaagctat ctcatttata attccaacaa cttttttatg
    44401 tgattttaac aacaaaagcc taggaatata atttaactta tctgtctttt ttatagcatc
    44461 aattatagat aaatttccag ctttatatat tggtactaca gaggctaata taactgctat
    44521 aattgacatg atgatagatt caattataga gtttgtattt atagttaatt ctgcactttt
    44581 atcatatact atcatgagac ctaagtaaga aaaaactata cctccaagta aaccaattac
    44641 tatacctaca gataaaacta caatactctg aataaaaaat acctgtctaa tatttctctt
    44701 gctagctcca acagctctaa gtaaaccaat ttgcttggtc atatctagta ttattatgtt
    44761 aaacatattg tatattacta atgttgcaca taataaaggg agtaagcttt gctttattga
    44821 ataagttgtt ttttttgaaa tatttttcat atacatagac atacttactt ctgtatttgg
    44881 ataaaattga ttacttgtta acttatattt tctccctaga ttcactactt tttgagaatc
    44941 taaatctttt gtattcaatt ttaatgtacc catatgagtt attaattcat atggaacaat
    45001 atctttgcca ttgtttgaat atgtaaaagc tcttacctta tatttattta aattttcata
    45061 gtattcatca gatttactta aaataccaac tattttaaat tgcttatctt tactataaat
    45121 agaattttca ttatttttat ttatatgctc attaacaatc tgaaaatcaa tagtttgatt
    45181 taatttgaca tctaattcca ttttgtctaa aactttttgt tctattacta attcattaga
    45241 agttctaggc tctctacctt taactaactt atatctagta gaatctaaaa aatctttatt
    45301 gtaagagcat aaatttagag atgctccatt atctttactt ataatttctc caaatttaag
    45361 tacatcattg aattctttta tatcttcttc accattaagt tcttttacta tatcactatt
    45421 tatatcttca taaaaagcat gataatctcc cgagtttttg tatgcatgat ttatatctga
    45481 ttgtccttga gattcattaa taacacttaa tgaaaaaact agcataacag caatccctat
    45541 actaagtatt aatgataaag tttttccttt ttgtctcttt aaatatgaca ccgcaagttt
    45601 aatatttatc ataatcagaa tcctccttat tcatatttac aagaaaaact ctataaccta
    45661 aaatagtagg ttatagagca ctattcatta aatactaact aactcgttgt gctagttaaa
    45721 tttaattatt aaaaatacta gagttgagaa aatcacattt catttgtaaa aaactacatc
    45781 tagtactttc aacatattta atagactgat tagtattttt taataagcta ctgttccttt
    45841 tggagttgat cctgcacaat ccaccataca gtctatttgt gaaattattc catgcttatc
    45901 ataataccat ttacatggat ctgcttgcac ctcaacatct tgaagttcta ttttttcttc
    45961 gatttttttt atatgtatca taatacatat ccccctttac ttttgtttta tatttattat
    46021 aactagttaa actagattat aaactacata gtttttttgt tgtttccaca taaactcttt
    46081 ctaatccaca ataaattggt actgcacttt ggaatttacc tgttaccttt ttattgataa
    46141 gtttacatct ggttgacata caataatcaa gtctagtaca tccatcacac tcttcattct
    46201 tagatatata aatttttata aggttcttaa tttttttatc atctaatcca tcatatatat
    46261 ttcctagtaa aatatcttta tctcctacac atactgtaca tggatataca tctcctctag
    46321 tatctattgc ataactattt atccctccaa aacaatctcc ttttttctta tttataaatt
    46381 gtttatctaa aatagatata tctatatcag gataatcctt tttaatattc ataattttac
    46441 taatttgttc atttaatatt ttaatatgag aattatccca atttttatcc ataaaatctg
    46501 aaattggtgc gatattttta aacccttgtt ttgctatatc atatacactc tcataaagat
    46561 acttaacagt atttgagtta tatgtcattc ttgccctaac atatatacct ttatctataa
    46621 gtctttttcc attttccatt actttttcat aagtacctac tccgttagca tctattctat
    46681 ttatatcatg tatattcttt tttccatcaa tacttatcga tatgtttttt acattctttg
    46741 ctataaactc tattatttca tcatttataa tagttccatt tgtagttata tcaaataata
    46801 tctttttgtc actcaatcta tctactaaat ccctatgtat atatttaatc aaatcaaaat
    46861 ttataaatgg ttctcctcca tgtaaaacaa ttgttgtata atcactatta ctttctaatt
    46921 ctttattaca aataaaatct attgttttat cagcagtttc ataactcata ttttcttttt
    46981 ttttatcatt accttcataa caatatgtac atctcaaatt gcagtcttca gttacaaata
    47041 tagtaaattt caattaactt tttcatctcc ccatacagct gtatttaaat tgtgtttcat
    47101 catactacac catctatcca attccaattc attatcctta actgaatcta atatcgctgg
    47161 gcaatgccaa caaaaaatat ttacatcaca atctttacat ttttcaaagt tatatggata
    47221 aatactttct aatttaatca aagactttag atctcttgtt ttattttctt ttataacttt
    47281 tacaaattca ttgtcaaata tatttcccat tttatagtca tcctttatca gtgaaggaca
    47341 aggatataca tctccatcat aatttataaa tatttgagag tttcctgcat tacatgcaca
    47401 acatcccatt tttttatttt ttttatcttt acacattttt aattcattat ctaaaatata
    47461 cggaattgta attggaagaa ctgtatcttc aattaaatag ctttctttat tttctaggcc
    47521 tcttcctgtt ggaataaaat atctttcaac aaattctgtt ccaagctttt tatttaaatc
    47581 tttaaattgt ttatttaaat gagaattttt ttgtccaaat accatagaca gctttatatt
    47641 actgaatcca ttgtctttta gcagattaac agagcttatt actttattaa atacaccttt
    47701 tcctcgtgtt tcactacaag tattttcatc tactccatca atactaatat ctattttttc
    47761 aaaattagtt ataataaaat caacattatg ttcatctatt aaagttccat ttgtggataa
    47821 tattaatttg gtatcaggta atttttgttt tatatattta attatttcaa aaaaatcttc
    47881 tcttatcaat ggttctccac cagttataat tatatgagga gtatttaaaa ctgatattat
    47941 atctacgata ttttttaaat catatgtact aagtaaatct ttcttattag aagaagattc
    48001 aacacaacaa tgtttacaaa ataaattgca tctatttgtc aaacaaaaat aaattgcaac
    48061 accattgctt aacatatcaa agtttaaatt gttactttta aaaatttgat ttttgttcat
    48121 aatatctata agattaataa tatatttttt gtcactttca tcatcaaaat aatttacaaa
    48181 atcataaatt tttattttat tttcttgtcc taaaagaaaa tacttatata tttcttcaga
    48241 caatttaata tattttctaa atttcttatt ataaataatt actagttgat tttttctata
    48301 aataattaaa tcttctacat taaaaatcat ttcttcagaa tctatcacaa ctaagtcctc
    48361 ctcaaattag taaaatattt ttctttaata tttattacta atatcaactt tactatctaa
    48421 ttagttattt aattgttgtt tttatgttcc tagttataaa gtttatcaaa tttacatttt
    48481 atttttttat actatataga acttttggtc aataatgtta ccatactaag gtcgcatttg
    48541 cagataaaaa taactctatt tttctaaatt tatttaagat atatcatgca aataatataa
    48601 ctataaaacc tatcaacatt ttaaattcaa acaaatataa atcacatttt ttgttctatt
    48661 attttattga gtactaaaaa ggatgttatc ttattagcat agcattcaac catttttatc
    48721 acctccaaat atcctatgcc ttaaagatag catcttctat tttatctttc aatactactg
    48781 ctccaactgg gcaatttaga gccccaacta gaattagttc ctagttaatg gtatataaat
    48841 agtaattgta aacttgtttt tatctgagta tatttccaca tttccattat atttttctac
    48901 tgaacttttt acactactta ttcctattcc atgtaaaaag gaatctttct tacttgtaat
    48961 aaccttatta ttttttaata caacttcatt tatctttgta ttttcacatt ttattacaaa
    49021 taacctattt ataacagttc ctcttaattt aatctttttt tgtatacttg tgtccttaat
    49081 cttaatacaa gcttctattg cattatctat tatatttgaa aatatactac atatatctgg
    49141 catctctata aaattacatt ctaaaaaatt aatatctact agaaaatata tactattcat
    49201 atcacaaata gattttttat cacttaaaat aacatctaga atcatattct gagtattaaa
    49261 tatagtatta cattctttta tttgcttatt tatatcttta atatacttat ctgcaaactc
    49321 atttttccca taaacattct gaatacaaat gatatgatta ttcatatcat gatatagttt
    49381 tcttattttc aactgatatt cctgtaaatt taaataatat ttatattgca tattcattct
    49441 tttttttgta atttcatatt ctatctttaa actattatct tttattattc ttcctattat
    49501 gcttactaat gaaagatttg atagtaaaaa tacagttgat attgtaagta ttattaaact
    49561 ctctttagaa tcgataatct tatctttaaa aataaattca aatattacta ttacaccaat
    49621 aatgtttgct attaatggaa tagataaata tatacaatcc ctcttgttta tttttgtttt
    49681 taatctgata aacttaagca atggtattaa tactattagt aatgatttag atatcattat
    49741 taactctaac ttcaacaaat tatcctctaa taaatcattc atatttttta tagaattaag
    49801 tgtcattact atacttaatc caatactatc aaatccaatt aataacatcc aatatagcaa
    49861 acttataaat acacttttcc atttatccac tacataattt attttataca atacatatgt
    49921 aataaatata catataaata atttaatatt tatattaaac tcaattgtat tcaacatagt
    49981 aattatcata atagcaatta ctatgctact atttagggtt aatctattct ttcttaactc
    50041 actaaattca tttaataaaa attttaatac aatccattct atcgtcgtag aaaatatagg
    50101 taaaaaaatc caaaatacat ttatatttaa cataatacat ctcccaatac atatgttaat
    50161 tttttcttta aattagccat tctatattta ctaactggta tatctttacc attaataact
    50221 acagtatttt tacatataaa ttgaatatac tccaaattaa ttaaatagct cttgtaacat
    50281 ttttaaaaat ttattctttc ttaaatcact gaattcattt gataaaaatt ttaatacaat
    50341 tcattctatc gccgtagaaa atataggtaa aaaaatccaa aatacattta tatctaacat
    50401 aatacatctc ccaatacata tgttaatttt ttctttaaat cagccattct atatttacta
    50461 actggtacat cttcatcatt aataaccaca gtatttttac atataaattg aatatactcc
    50521 aaattaatta aatagctctt atgacatcta aaaaaattat atttttctag ctctaattct
    50581 attttatcca tactattttt agtataataa gtatcatttt tagtatgtat agtcaaaacc
    50641 tttttcctaa cctctatata cataatacta ttaattaaaa ttttattgat tataccttta
    50701 ttttctatca gcataaaatt ttctcttttt tttataatat ccgatataca actcaaaata
    50761 ctttcattta aatctccaaa atctataggc tttaataaat atctataagc ccttacctta
    50821 tagccttcct gtatgtaatc aactattgaa gttataaata ttatttcaga agtatcatta
    50881 gtttctctaa ttattttagc cacatccatg cctgttagtt gaagcatttt aatatctaaa
    50941 aaaaatatat caataccttc taaatttgtt tccaataatt cttctcctga attaaactca
    51001 aatagttcaa tttgactaga aataccctct aaggacttta atatacactc tcttaaaaaa
    51061 gctatttgtg taatatcatc ctcacagata actattctat acataaacac ccctccttag
    51121 acattagatc tgaaaacaaa taaaatatca atttataaat aattatttag ttatacaact
    51181 aacatggtca tcaaatacta ttattcactt aaaaaatcac cccctatttt tagtatgtta
    51241 acaacaaaat aagaagatat cttagatttt taaaaacaat taatacttaa tgtagttttg
    51301 tatactattt ataggtaatt ttttatatca ttttaaacat taaaattatt gatgttt
//
LOCUS       DAMJYC010000023        48602 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJYC010000023 DAMJYC010000000
VERSION     DAMJYC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.024"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            62..796
                     /locus_tag="PC472_001378"
     CDS             62..796
                     /locus_tag="PC472_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2726711.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(939..1430)
                     /locus_tag="PC472_001379"
     CDS             complement(939..1430)
                     /locus_tag="PC472_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2726712.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1779..2162
                     /locus_tag="PC472_001380"
     CDS             1779..2162
                     /locus_tag="PC472_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726713.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            2670..3053
                     /locus_tag="PC472_001381"
     CDS             2670..3053
                     /locus_tag="PC472_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2726714.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            3055..3405
                     /locus_tag="PC472_001382"
     CDS             3055..3405
                     /locus_tag="PC472_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726715.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(3506..3964)
                     /locus_tag="PC472_001383"
     CDS             complement(3506..3964)
                     /locus_tag="PC472_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726716.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLKEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(4009..4797)
                     /locus_tag="PC472_001384"
     CDS             complement(4009..4797)
                     /locus_tag="PC472_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2726717.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(4936..5373)
                     /locus_tag="PC472_001385"
     CDS             complement(4936..5373)
                     /locus_tag="PC472_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2726718.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            6195..6704
                     /locus_tag="PC472_001386"
     CDS             6195..6704
                     /locus_tag="PC472_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726719.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVELEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFVTRKKI"
     gene            6731..6880
                     /locus_tag="PC472_001387"
                     /pseudo
     CDS             6731..6880
                     /locus_tag="PC472_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            7109..7645
                     /locus_tag="PC472_001388"
     CDS             7109..7645
                     /locus_tag="PC472_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2726720.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKI"
     gene            complement(8079..8879)
                     /locus_tag="PC472_001389"
     CDS             complement(8079..8879)
                     /locus_tag="PC472_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726721.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(9074..10672)
                     /locus_tag="PC472_001390"
     CDS             complement(9074..10672)
                     /locus_tag="PC472_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2726722.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            11000..11176
                     /locus_tag="PC472_001391"
     CDS             11000..11176
                     /locus_tag="PC472_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726723.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            11350..12480
                     /locus_tag="PC472_001392"
     CDS             11350..12480
                     /locus_tag="PC472_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2726724.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            12721..13071
                     /locus_tag="PC472_001393"
     CDS             12721..13071
                     /locus_tag="PC472_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2726725.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            13079..13528
                     /locus_tag="PC472_001394"
     CDS             13079..13528
                     /locus_tag="PC472_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2726726.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            13630..14205
                     /locus_tag="PC472_001395"
     CDS             13630..14205
                     /locus_tag="PC472_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2726727.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            14198..15607
                     /locus_tag="PC472_001396"
     CDS             14198..15607
                     /locus_tag="PC472_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2726728.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            15791..17224
                     /gene="eutA"
                     /locus_tag="PC472_001397"
     CDS             15791..17224
                     /gene="eutA"
                     /locus_tag="PC472_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2726729.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            17242..18606
                     /locus_tag="PC472_001398"
     CDS             17242..18606
                     /locus_tag="PC472_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2726730.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            18619..19500
                     /gene="eutC"
                     /locus_tag="PC472_001399"
     CDS             18619..19500
                     /gene="eutC"
                     /locus_tag="PC472_001399"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2726731.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            19520..20173
                     /gene="eutL"
                     /locus_tag="PC472_001400"
     CDS             19520..20173
                     /gene="eutL"
                     /locus_tag="PC472_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2726732.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            20184..20885
                     /locus_tag="PC472_001401"
     CDS             20184..20885
                     /locus_tag="PC472_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2726733.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            20888..22357
                     /locus_tag="PC472_001402"
     CDS             20888..22357
                     /locus_tag="PC472_001402"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2726734.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     IPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            22453..22740
                     /gene="eutM"
                     /locus_tag="PC472_001403"
     CDS             22453..22740
                     /gene="eutM"
                     /locus_tag="PC472_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2726735.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            22867..23628
                     /locus_tag="PC472_001404"
     CDS             22867..23628
                     /locus_tag="PC472_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2726736.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            23641..24270
                     /locus_tag="PC472_001405"
     CDS             23641..24270
                     /locus_tag="PC472_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2726737.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     RYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRSLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            24312..25037
                     /locus_tag="PC472_001406"
     CDS             24312..25037
                     /locus_tag="PC472_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2726738.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKKDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            25050..25322
                     /locus_tag="PC472_001407"
     CDS             25050..25322
                     /locus_tag="PC472_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2726739.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            25315..25866
                     /locus_tag="PC472_001408"
     CDS             25315..25866
                     /locus_tag="PC472_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2726740.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            25878..26969
                     /gene="eutH"
                     /locus_tag="PC472_001409"
     CDS             25878..26969
                     /gene="eutH"
                     /locus_tag="PC472_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2726741.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            26971..27444
                     /locus_tag="PC472_001410"
     CDS             26971..27444
                     /locus_tag="PC472_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2726742.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(27564..27665)
                     /locus_tag="PC472_001411"
                     /pseudo
     CDS             complement(27564..27665)
                     /locus_tag="PC472_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            27960..28481
                     /locus_tag="PC472_001412"
     CDS             27960..28481
                     /locus_tag="PC472_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2726743.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            28860..30035
                     /locus_tag="PC472_001413"
     CDS             28860..30035
                     /locus_tag="PC472_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2726744.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLIFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            30069..31607
                     /locus_tag="PC472_001414"
     CDS             30069..31607
                     /locus_tag="PC472_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2726745.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            31639..32841
                     /locus_tag="PC472_001415"
     CDS             31639..32841
                     /locus_tag="PC472_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2726746.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKNENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(33008..33607)
                     /locus_tag="PC472_001416"
     CDS             complement(33008..33607)
                     /locus_tag="PC472_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2726747.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARYIKVQL"
     gene            33777..35135
                     /locus_tag="PC472_001417"
     CDS             33777..35135
                     /locus_tag="PC472_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726748.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            35128..35760
                     /locus_tag="PC472_001418"
     CDS             35128..35760
                     /locus_tag="PC472_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2726749.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            36089..37246
                     /locus_tag="PC472_001419"
     CDS             36089..37246
                     /locus_tag="PC472_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2726750.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(37393..38028)
                     /gene="lexA"
                     /locus_tag="PC472_001420"
     CDS             complement(37393..38028)
                     /gene="lexA"
                     /locus_tag="PC472_001420"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2726751.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(38132..39259)
                     /locus_tag="PC472_001421"
     CDS             complement(38132..39259)
                     /locus_tag="PC472_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2726752.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(39580..40425)
                     /locus_tag="PC472_001422"
     CDS             complement(39580..40425)
                     /locus_tag="PC472_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726753.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(40455..40826)
                     /locus_tag="PC472_001423"
     CDS             complement(40455..40826)
                     /locus_tag="PC472_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2726754.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(40979..41239)
                     /locus_tag="PC472_001424"
     CDS             complement(40979..41239)
                     /locus_tag="PC472_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2726755.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(41428..42372)
                     /locus_tag="PC472_001425"
     CDS             complement(41428..42372)
                     /locus_tag="PC472_001425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2726756.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(42393..43247)
                     /locus_tag="PC472_001426"
     CDS             complement(42393..43247)
                     /locus_tag="PC472_001426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2726757.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(43262..44623)
                     /gene="accC"
                     /locus_tag="PC472_001427"
     CDS             complement(43262..44623)
                     /gene="accC"
                     /locus_tag="PC472_001427"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2726758.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(44639..45091)
                     /gene="accB"
                     /locus_tag="PC472_001428"
     CDS             complement(44639..45091)
                     /gene="accB"
                     /locus_tag="PC472_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2726759.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(45361..45807)
                     /locus_tag="PC472_001429"
     CDS             complement(45361..45807)
                     /locus_tag="PC472_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726760.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            46082..46360
                     /locus_tag="PC472_001430"
     CDS             46082..46360
                     /locus_tag="PC472_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726761.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(46489..47235)
                     /locus_tag="PC472_001431"
     CDS             complement(46489..47235)
                     /locus_tag="PC472_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726762.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(47364..48008)
                     /gene="trmB"
                     /locus_tag="PC472_001432"
     CDS             complement(47364..48008)
                     /gene="trmB"
                     /locus_tag="PC472_001432"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2726763.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(48146..48466)
                     /locus_tag="PC472_001433"
     CDS             complement(48146..48466)
                     /locus_tag="PC472_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2726764.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        18396 a         6420 c         7396 g        16390 t
ORIGIN      
        1 cttatatttt gtatttaata gagtgttgct aagtgaatat atttaaaatg gaggaaactt
       61 tatgaaacaa aataaatatg atgatgatag attttttaat aaatacagta aaatggaacg
      121 ttctacaaat ggattagcag gtgctggaga gtggcatgtt ttaaaaaaaa tgttacctga
      181 tttcaaagga aaaagagttt tagatttagg atgtggattt ggatggcatt gtcaatatgc
      241 agttgagaat ggagcaattt ctgctgttgg agttgatatt tcagaaaaaa tgttaaaaga
      301 agctagaaac aaaacgcaac ttgataatat aaattacata tgtatgccta ttgaagatat
      361 taattttcca aaagatagtt ttgatgtagt tattagttcc ttggcatttc attatattca
      421 atcatttgaa gatatatgta agaatataag taattgtctt tcaaataaag gtaattttgt
      481 attttctgtt gaacatccta tatttactgc atatggaagt caagagtggt actgtgataa
      541 agatggcact aatctacact ggccagtaga tagatatttt gaagaaggag tacgtaagtc
      601 aaattttcta ggagaagagg ttataaaata tcataaaaca ttaacaactt atttaaatac
      661 gttaatgaaa acaggatttg aaatattaga aattattgaa cctcaaccag aagaaaaact
      721 gttagataca attcctgata tgaaagatga gctacgtaga cctatgatgt tacttgtatc
      781 tgcaaaaaaa agataagtaa atataaataa tcattattgt agaaaaaaag ttgtagaaaa
      841 taattacact aatggagatg attataccag tggtattaat tacatcaata gtaaggaggc
      901 aaaagcattc gttgcttttg ccatttttta agttgcaatt atacttctac gtagtatatt
      961 tcgtttccat tttcttctat aagctttttc atatcttcaa attttaaaaa tacagtagca
     1021 gtattatcat ttggatgtat acctatttta tccattttta ataaatcctt atcaaataca
     1081 acttttacaa cagcctcttt atcatttaat ataccaaaag gagtcacagc tcctttagta
     1141 agtttcaagt atttttgaag tctttcttct gatgcaaaac taagaggttt actatcaatt
     1201 tgttgacgaa tatcttttaa atctgctttt ttgtctttac ctaatacaac taaaaaatgt
     1261 tcttttcctt tagcatcacg aagaaatagg ttttttacaa caaattccat atgtaatgtt
     1321 tttaattctt ccatttcttc aattgtataa actggtggat gatttacagc ctcataaggt
     1381 atgtttaact catctaattt tttatatact tcctgttgtt gattgttcat gatttcctcc
     1441 cattagaaat ttattttata tataaataat attatcacag aaaactttaa aagaaaacgt
     1501 tttatataat atagacaata gattatattt ttataaagaa gcaagaatac tcaataaaac
     1561 ttaaagaaat taaacaattt ctaaaaatac cttgaaaata gaaccttatt tgccatttat
     1621 aaattatata aaaagggatt atataaagtc atatatgaaa aaactatatt atactatctt
     1681 ttctttttgg aataaactat actttaaata tagatattta atttaaataa attttacagt
     1741 attaatagta aattatctta gaaaggaatg tgctttatat gtctacagac aaagttgttg
     1801 gaatcataga tgaagaaacg gcagaacttg caggaattga atatactggt aaaatttatg
     1861 cttcttctgg tgttataaaa catataaaga aaaaacatag atgtcaactg agtaaggata
     1921 tttttaatga tataatagat actataaaaa tggtattaaa gtcacctgaa tatataggaa
     1981 gtcatcctaa aaaacctgga aaaagtgtgg agtttataaa aaaaattgat gattatattt
     2041 tagtagctac agaattagat acaaaaaaag gatatttata tgtatcttca atgtatgcta
     2101 taaaagaagc aaaattagaa agtagagtaa acagtggaag agtaaaacaa tatgatggtt
     2161 aacataatat tattaatttt ccctaaaata ttgaaaaata ttattatata agttataata
     2221 ttaatataca cgtagatatt tattagttag atttgaggtt ggaaaaggca cccaatacgc
     2281 tactatagag tagttaatag agatgcagga tacgccgccc tgccaaatct aattaaatta
     2341 aaaagagagg attaattcct ctcttattgt ttttttgaga tactaattta atctataata
     2401 gaaagattga aggtcgttta tacaaaaaaa ttttcagtta tatacattaa actaattaaa
     2461 taagatattt ataatctata tgtgatacat attatatatt aataaaagaa aaatatatag
     2521 aaaatcctta taaaaattta aattgtaatg aataggaagt aattaaaaag aatttttatt
     2581 gatttttaaa aatttagtta gaaactatat cattatggta aaaaatgtat tataatgttt
     2641 agaaatatta taataaggag cgattaaaca tgaatctatc gaagaaaata gaagataaaa
     2701 gaaaagctaa acagagagct gaaaaagtta agaaagctaa aattgctaca gctggtgtgg
     2761 tattaggagc tgttactggt gcagtgagtg gagtactttt ggctcctaaa tctggaaaag
     2821 aaacaagaga ggatataaaa gatgcttcac aacaaattgc tgaaaagata aatatgaaaa
     2881 ctgtagatgt aaaaggcaaa gtatctgaga agctagagga taaaaaagga aactttatag
     2941 aatcaaagaa aaaaataaaa aaatatatag ataacaaaaa gagtgttgaa aattctagtg
     3001 aggaagataa aataatagat atagttgaac cagttagtgt tactgaggaa taatatggac
     3061 aatacaataa cgattaacat aagtataata tatgtattat tagctatact tgcttgtatt
     3121 gctttagttt atttaattat tgtacttgtt aaactacata agattttaaa taatgttgga
     3181 actatgttaa atgaaaatac tcaaaatata aatacaactt taaataatct tcctaatgtt
     3241 gtagaaactt ttggagaagt aggagaaaat atgaaagatg taacagaagt agttactgaa
     3301 gttacagcag attttcttgt tactaaagac agtgtcaaat caaatatgga tgttatagtt
     3361 gatatattga ctataattaa aaacattttt ctagataaaa aataataagt taatataaaa
     3421 taagcctata agatataatt taaacgacct taaaatagca tttataaggt cgttttttat
     3481 tagcttgatt tgacactatg tgtttttaat aattggtaat attcctcttt atcatttgaa
     3541 catatatatc tatgcttgtt gtttcaatta catccataag aacatcttta cattcaagct
     3601 caaaatcttc cattccttct aaatattcat catcttcatc aaaaaagata taagaattat
     3661 atagccattc ttcaaatttt tcttcaagta tatcccaata cgaactccaa aataatccac
     3721 aacctgatat ataagatgct ctactttgat ttatataaac ctcctttaga agcttttgta
     3781 atctactgct ataaagaaaa tctgctcttc cactgcttga tattaattca tttaaatctt
     3841 ctattgacat taattcaact ttaccacttt tatcataagg agtataattt tcatcaaccc
     3901 atttatgtgg tttgttttct ctgcatactg ttgctatcaa ctctacaact tttgttctta
     3961 tcattttttt atcctaactt atttattttt tataaaataa tcatgatttt ataatttatc
     4021 tatttcttgt aatgctaatt taacagtttc atatctatca gcaccattta ttactacatc
     4081 accttttaca agtttattag cttctccacc tacaacataa acctttttac ctaaaccttg
     4141 cttataacta gacgcctcaa ttattaaaga atctttcaat tgccaatgta aaatctgagc
     4201 agccacttta tcagcatcac ctgtataaac gattacattt tcatacactt tattttcccc
     4261 tcccccttga gaatttgata tagatttgtt tagtatacct tctgctatca atttagcaac
     4321 tttatcttta ttattaacat aataatctgt atctgcttta ctatctacaa aacatacttc
     4381 tattaatatt gctggtgcaa tagtatgatt aagccaataa agacctctaa catcagactt
     4441 tgcaccccta tctttgaata ctgtagctaa cctagtattc actctttctg cataggtttt
     4501 gccattatta gtcttatata ttgtttctgt ccctactgga gttgaagttg tagcatttga
     4561 attaaaatga atctgaacag ccaaatcagt ttcctgttta tttgctatag cacattggtc
     4621 tgctaaatga ttacttgatt catcaactct tcctgtataa acagtagctc cacctatttt
     4681 aagccattta acaattaagt cattcagtat tcttgtttct ttgctttcgt ttatataccc
     4741 aacagctcct gtaccttttc caattaaagt gtgccctggt attattacta ctttcaaact
     4801 aacatctcct ttacaattaa atttaacttc tatttaatat atgcataacc tagaattttg
     4861 tttcttttta tatcttatac ttttattata acccattttg taaaaatata gaatattaag
     4921 taaataatgt taaaattata attttgtata taagacatta cttgattgca ataattttat
     4981 agcatattca tctattctat atccagttct ataatatata tgtttaattc cagcttgaat
     5041 tatagcctta gtacaattta agcaaggaaa gtgagttaca taaatagagc aatctttaac
     5101 tgaaatacct tcttttgcac aataataaag ggcattttgt tcagcgtgaa tggttctaat
     5161 acaatgtcca tctctcattt catgaccaac ttcactacaa tgcttatctc cagaaatact
     5221 accattatat ccagtagaca ctattctgtt ttctgaatta acaattatag ctcctacata
     5281 agctctatca caagttcctc tttcagctac tgtttcgcac agtctcataa aatattcttg
     5341 ccatgaacat ctattttcaa gttttttatt caaatcatat acctccaaaa tttatatcta
     5401 atatataaga tttatacctt aaaaataata ttaaaaacaa atttatacga taaataaaat
     5461 ttattaatac tgtttttagt ctaaattatt tcattttaaa ttaaagttac aaggaatcta
     5521 aaattatata tatctttctt cgtaatatga atgttacaat ataaaaaact gtctcaataa
     5581 aatagagaca gttttacagt caaagtttat aatacaagtt catatttttc tttaaattct
     5641 tttacagctt cgggccatgc cttttgttct gtaatacctt tttctttagc tattttaatt
     5701 gcaatctctc ttatcattgc agcgccttct aatattccca tttttaatct cccttcaaat
     5761 atctttttgt atagaatata aatagtgcta agatttatta catgctgcat atacaatatc
     5821 tatacaacac ataacttgta aattcctttt attttcatac gtctttaacc gacatataat
     5881 tatattcaat aagttttaaa attatgaact caattataat tatttttatt tttttagaat
     5941 ctaaacacta aaaattgtag gtaaatttgt cttatgcctt tataaatatt tctacagttt
     6001 taattactgg acatatctat ttttattata aaaagcgctt aaaaatcgag aatatccgca
     6061 taaaaactgt atataagcaa aacggttaaa ggaaaaagaa gaaatttact tgaatgttaa
     6121 aatgcgttgc ttgaagcaat gggcagtttt gcctataata tcggtaacaa ctgaaagaaa
     6181 ggtggttagc cctaatgtta agcgaaaaca ttaaaacaat caggaagtca aaaggacttt
     6241 cacaacaaga acttgctata aaactgaatg tagtgcgaca aacaatatct aaatgggaac
     6301 aaggactgtc agttcccgat tctgatatgt tgatttccat atcagaggtg cttgaaacgc
     6361 ccgtaagcac tttacttgga gaaactattg ttgagttgga agttgatagc ttaaaagtga
     6421 tttctgaaaa attagagatt ataaacttgc aacttgcaca aagaaaaaac atgagaagaa
     6481 aaatgtttca ttggttattt attttattat gtgcagtcat attaataggt tttgtggtct
     6541 tagggggact aaatagtcct tacttggatt ggaattatac cgaccctgaa accgctgttt
     6601 ttggagtagc ttttcatgct tttgagtggc tatttgtcag attagtaccg attgtcctta
     6661 taggagcaat tattggaatc tttgtgacac gtaagaaaat atgaagtaac tctgcttttt
     6721 ataatacagc aagcgttcct taaaagcgtc attctacatg gcaatcgagg acgactatgt
     6781 aaggaagaac ctttttgatt tccaactaag cgaagttatt gaggacgaca gtaggctcaa
     6841 aaccgcatta agtaaagtac aggaagcaag actgctggac ttatactctc atgcgtcaga
     6901 tgcaggaaat gcgaggtctg gtagcatgat ttacaacgat ttgtacaacg actacgagta
     6961 gaaatatatg gagataagta ttatctacga aatcagaaaa gaaatgactt gataatttac
     7021 ccatgttata attattgatg tgaaattaat aaatcgaaat tcctttttta gatttaacaa
     7081 tactgagtaa ggaaaaagtg aatgactttt gcaaggaggt gttaatatca ggcaagtaaa
     7141 gtcttttgtt tttccagtta tatccttaat attcttagac caaattagca aagttcttat
     7201 aggattattc ttaatggact ttgaaattga tataattggg aaatttttaa gattcaatcc
     7261 tgttcaaaat acaaatctct cttatggggg aaactttatt ggtattctat ctaatttatg
     7321 ggtattggta ttgtttaaca ttttagttat attagttatt atatctggat atgcttttta
     7381 taaatcaaaa aatgaacaaa caagctattc agtaaaagta attatgtctt gtggacttgc
     7441 tggtacaata tgtagcttga tagataaatt attttgggga ggaagtttag attttttgca
     7501 gataccaagc ttttttattt tcgacttaaa agactgctac cttactgttg cagaaataat
     7561 atttgttgtc ataggaattt tgcataatag agaaatatca atgaaagaat acatatattt
     7621 ttgctatcgt cagtttaaaa tataaattta gaagataaat ttaagtttca aggtaaactt
     7681 atttaatcac agcaaatagt tggcttactt aagcttttgg gctaaactgt gagcactaat
     7741 taagtctctg ctatctaaca aacggagcac aaaaataaaa attaaatatt aaatataaag
     7801 cacagttaat tttgattaaa aagttgactg tcttttcttt tgtagaaatt taagcatagg
     7861 taataaagaa atgactgcat ttaagcatta tgtatataat tggaggcaat aaaaagccag
     7921 caaagcccat aaaatagagc tgtgctagca acatgatgag ttataagaag ataatcaaaa
     7981 acttacgctt tattttaatt taaaaaaatg attactgtaa agaaatcatt ttttattata
     8041 atcactgata catcaaatat tctaaataaa atttttattt atctggaatt gtatattttt
     8101 tctataatat taactacagc gtacattgcc caagcacaga tagccaatac aaatactccc
     8161 atcattacta aatctaattt aaatacttga gaaccatata ctatcaagta gcctattcca
     8221 tatcttgaaa ctaaaaattc tccaactatt acacctaccc atgccatacc aatattaatt
     8281 ttagttaaat ttattaaatt tcctgtgttt gcaggtaata tgagtttaaa taatatttga
     8341 gatttagtag ctccaaagct ttttaacatc ttaatttttt cttcatctat attcataaaa
     8401 taattataag ctgaaagtat agtaacaaca acagaaatcg ttacagctgt tacgacgatt
     8461 ccttctattc cagcacctac ccaaactata attataggag ctaaagcagt cttaggaaga
     8521 gcatttaaaa ctaccaaaaa tgggtctaat atttttgata atttttctga ccaccaaaga
     8581 gcaattgcta ccaaaatacc taatacagta cctataacaa gaccaagaac agtttcatat
     8641 acagatattc caatatgttt aaataattga ccactaaatg catattgaat aaataaattg
     8701 taaatatcac ttggtttgct aaatagaaat gtatttataa catttaagtc agctaaaagt
     8761 tcccagatta caataaaacc aattaaaatt aatagctggt agaataatac atttcttttt
     8821 tctcttttta cacctttaac atagtttata tatccttcag atcttttatt aagcctcatg
     8881 tgtatctagc tccttccata atatgtcgaa atacttttga aaatcaggaa ctttacgtgt
     8941 tttgagaggc gtttttgttc catccatgtt aagttcaata tcgtaaatat tttttatggt
     9001 agctggtctt ttagatagta ttgcaacctt atctgatatg cttattgctt ctgctatagc
     9061 taaaaaagtg caactaattg cttaaagaaa cagaattatc cacttcagca cccatataaa
     9121 caatattaag atttcctaaa ctgctatccc aaacgatact attaataata gtagataata
     9181 acaatctctt atcatctaaa ctggcattat caacttcatc attaaatctc ttcaaattac
     9241 taacaataat atcaatatta atactgtcac tactaatatt gatgttacta gattccaaag
     9301 tattaatctt atcctttaaa atactaatct cattatttag gctctcaatc tgggaaacta
     9361 tataatcaga agcagtacta ccagtatttt ttgcaagctc tataactaaa tttttaataa
     9421 gtctttcctt atctaaaatt tgatttttaa gtaaattagt ctcagattta aaaatattaa
     9481 aaacattatc agtcttaatg ttattactat tgaatgcact tataatagac tcttctttac
     9541 aattcttaat tttatctata actaaacttt caactaatgg accattaaga tttttacagt
     9601 tgcaactatc agacccaagt gtttttttag taccacaaat ataataatac tttaaatcct
     9661 tgtcagtttt tttgccttta taggtaattc tcatattaga accacattta ccacatttta
     9721 taagaccaga taaaagagca gtttttccag ttgatatcct tggagtttta tcacgatttt
     9781 tatctaattg attttgtact tcaagccata tttcaggttc tatgactcca tggtgttttg
     9841 caattgctgc tattgagtta gatgtatttt tagcataggt aaggattcca cattttctat
     9901 ctggagtacc tacaacatcc ataccactat ttttaaggta atctaaaatt tcatcattag
     9961 cttttacata tgctggattt ctaagtattc tagataaaga actagggtca aatttaccac
    10021 cacgaggacc ccttattcca ttattataga catatttata taacttagat aaggacctaa
    10081 gttctaaata tttattgtat attagcttta caatctccat ttgttcatca tttacttgaa
    10141 gcttatacat acttctttct tttagatttt catctaagaa tgtgattttt tcactgttga
    10201 atccatgagg tgtattgcct ccaagccatc taccactacg ggctaattca tacatattgt
    10261 cttttatacg ttctgctatt gtttctcttt ctaattgagc aaatacactt gatataaaca
    10321 tcatagctct tcccattgga gttgaagtat caaattgttc tttgatagat ataaatgata
    10381 tgtttagttc atttaatttt tctattaaag tagagaagtc agatacattt ctcgatatcc
    10441 tatctaatct ataacaaact aggtaactaa atttcttatc tttggcatcc tgcatcattt
    10501 tcttaaattg tggtctatct atattgctac cactaaatcc ctcatcttca tatatcaaaa
    10561 aatcttttac accaatagac tttgcataat caatacataa ttgtatttga ttttctatag
    10621 aatcaccttt tccagtaaat cttgattttc ttgaataaat tgcagctttc aaaattaaaa
    10681 cctcctataa gttaggatat atatttgaaa ctatttatct acaacctttt aagtaaactt
    10741 attagaatga atttattaat taggtaaatt tatataattc tactttttat aatatatgac
    10801 tatttatttt atttctttac tattcaagtt ctaaatcaat aataaatttt gatatttaat
    10861 tgtatcagga caaatctcta atttcataga ttattaatat aaaatcatag gttagtgtga
    10921 aaaagttgaa atttaaatat tttaaaagat aatttttttg aactaatagg tataacaaaa
    10981 atttataggg ggattgcata tggaaatcaa tgttatagaa attttcccta aagataaagc
    11041 taaacttaat aaaatagaaa tggataaagc tagttggttt gtaaatataa taagtaaaaa
    11101 atatcctaaa gaagctttaa atgaagcatt tagtacttta gaaaaagaat taaatataag
    11161 taaagctaat acataagctt tgattattga ttttatatac tattggtttt atacactatg
    11221 acttgacatt atattaatca aatagtatat ttttaaatat agcacaatga agtgttaaaa
    11281 gagcaatgga gctccataga taaagtctat ggagcttttt tgcgtattta acttaaggag
    11341 gataggactt tgaataactt tgagattaaa actagaataa aatttggaga aggctcacta
    11401 aaagctttgc aagaattaaa aaacaaaaat gtactaataa taacagaccc ttttatggtg
    11461 aaatcaaaaa caatagataa aataacatct aatttaataa actcaacata tcaagttttt
    11521 gaagaaatag taccagaccc accaatagaa gtagtagtag caggaataga agcatttaaa
    11581 tctataaatc cagacgtaat aatagcatta ggtggtggtt ctgcaataga tgcagcaaaa
    11641 tctataatgg acttttctaa aaaaatatta aaaattaacg aagtagagtt tatagcaata
    11701 ccaacaacaa gtggaacagg ttcagaagta acatcatttg ctgtaataac agatagtcaa
    11761 aaaaaatgta agtatccatt agtgagtgat gatttacttc cagatatggc aatactagac
    11821 ccagaacttg taaaaactgt accaaatttt ataacagcag atacagggat ggatgtattg
    11881 acacatgcta tagaagctta cgtatctaca aattctaatg acttttcaga tgcactagct
    11941 gaaaaagcta taaagttagt atttaaatac ttaataaaag catataaaga tggaaatgac
    12001 atagaagcta gagaaaaaat gcacaatgca tcttgtttag caggtatggc atttaaccaa
    12061 gcatcacttg gcataaatca ttctattgca catgtattag gtggaaaatt ccatatacca
    12121 catggaagaa caaactctat attacttcct catgtaatag aatataattc atcaatagta
    12181 ggatatacta acacaactta ctctgctaca gctaaaaaat atgcacaaat agcagaatta
    12241 gtaggacttg ggagtgctaa tataagaata tctgttagaa atttagtaaa tgaaataaag
    12301 aaactacaaa aagaaatgaa tatgccaact aaattgacac aatgcaaatt aagtttagaa
    12361 gatataacta atgaagaaag agaaatagca aaacttgcta taaaagatac ttgtacatta
    12421 actaatccta aggtaccaac tgaagatgat ataataaaca tattaaagaa cattaaataa
    12481 ctacatttta ttacatgtaa atataatatt atacaaatag ggaaaatgtt tttaaaaata
    12541 taaattatat attgacaaat ataaaacata aaactataat gaaagtatca caaaggcgtg
    12601 atactttagt agaaagcaat ggagcttctt aggaatatta atattattct tatggagctc
    12661 cttttatttt aatcaaatag ctttttaata aactatattc aaagaaagca gggaataaat
    12721 ttgactgaag aatctaagca aagagttatt caagaatatg ttcctggaaa acaggttaca
    12781 ttagcacata tcatagctaa ccctaatgaa gatatatata agaagttagg attagtactt
    12841 gataaaaaag atgcaatagg aatacttaca ataacaccaa gtgaagcgtc aataatagca
    12901 gcagatgttg ctacaaaggc ttcaaatgta tctttaggtt ttatagatag atttagtggt
    12961 tcagtagtaa tatcaggtga tgtaagttct gtagaatcag cgttaaatga tgttttagaa
    13021 gtcttaggaa atatgttaaa cttctcatct acaaaaatca caaggacata ataatacaat
    13081 gaagaatata atatttatgg gaaagacagg tagtggtaaa actaccttat gtcaaaaact
    13141 agaccagtta gaattaaagt ataaaaaaac tcaatcagtg gaactttata ataatgcaat
    13201 agacactcct ggtgagtata tggaaaatag aaacctatat aatgccttaa taacaacatc
    13261 agcagatgct aaaattatag caatagtgta tgatgccaca caagaagaaa attatatagc
    13321 accaggtttt gccagtatgt tttgcaaaga cgtaatagga attataacta aaataaataa
    13381 agctactgaa aacgaagtca atatagctta tgaaagacta tcactagcag gagcaagtca
    13441 aatctttaaa atagacacag ttgataatat tggacttgaa aaattattta aatatctaga
    13501 aaaatataaa aatatagatg aagtttaagg ggaaatattt ttttattcaa aaatattatt
    13561 aaagtatgat aaaataaaaa aatgtataaa ctattttaag aagatttaat ctttaagcga
    13621 ggtaggaaca tggggaaaaa aatactattg gttgatgatg aaccactgtt aaggatggat
    13681 acaaaagata tattagaaag tcatggctat gaaataatag gcgaagcaag tgatggattt
    13741 gaagctgttg aaatgtgtaa aaaacacaaa cctgatttag ttataatgga tatagagatg
    13801 ccaatacttg atggtataaa ggcaggcaaa ataatatgta aagaaaatat agctggtgga
    13861 gtacttttat taacttcatt tgaagataaa gaatacgtag aaaaggctaa aagtataggt
    13921 gcttatggat atttagtaaa aacagcaagt gaaaaatcac ttatcccaac catagaaatg
    13981 tgtttaaata aggtaaatga atttgaaaaa atgaaaaaag atttagaaaa agtaaattct
    14041 aaattgaatg aaagaaaatt agtagaaaga gcaaaaggta tacttataag agagtttaag
    14101 atttctgagg atgaagcata cactagaata agaaaattaa gtatggataa aagaactaca
    14161 atgatggaaa ttgccaaaat gattataata ggatacgatg actaatacga taaaaaaaat
    14221 atcaactgca caaacatact taaataataa cgaaattgaa aaaattatag aagtatcaaa
    14281 atccttagat ttaatggctt ctttttatga agctgatgtt tttatagatg tactaagtaa
    14341 atttgatgat gaagcaattg ttgtggcaca tggtagacct aaagaaaagt caatgtactg
    14401 tgaaaatgtt ataggtaaaa aggctttaat tgaaaatgaa ccaggagtta taaagtgctt
    14461 aaaaacagga gaaagagtaa gagatattaa agctttaaca caggaatata aactggtcaa
    14521 acaaaatatt cagccaataa cactagatga caaagtttta gcagtactaa tagttgaaaa
    14581 agatataagt agtgaagtta agaatgagtt taatgctagt gaagacgaag aaaataaaaa
    14641 tttacatcag tttataaaac ttattggaaa taatgacttt ttagttgata atttaaattc
    14701 agccatatta atatttgata aaaatggaaa attaaaaatt aaaaatcgta ttgcaactga
    14761 gatatacaaa aatcttgggt ttggatatga tatacaagat atgcattaca atgatttaga
    14821 tatagaaacc aacacctttg aaaatatatt aatggatgaa aattatgatg agacaaatga
    14881 agtttcaata ggtaatatgt tttttaaaat aaagaccata tacatcaaag atgatgaatt
    14941 taaggtttgc aaaatagtac aagatataag tgatttaaag agaaaagaag aagaattagt
    15001 tttgaaaaca gtggctataa gagaagctca tcatagagta aaaaataatc ttcatacagt
    15061 aatatctata ataaaaaaac aaagtagatt atctcaaaat gatgaagtaa agcagtgttt
    15121 agataacata ataaataggg tatttgcaat actttcgact cattatttac tatctaaaga
    15181 agttgataat aatatatcta tacgagaagg tatcaattta ttagtgtcaa atattcaagg
    15241 tggctattta tttagtaagg atatagatat acgtataact ggtgaagatt ttaaaattag
    15301 tggagaaaaa gcaacagcaa tacttttggt tataaatgaa atactacaaa attgttatga
    15361 ccatgcattt tcggatagag aacatggtac tatacaaata ttaataaata aagataacga
    15421 ctatagaaat attgctatta tagatgatgg aattggattt gatagtaata atataaataa
    15481 aaatagctta gggacatata taatagatag ttatataaca cagatgttaa aaggaaatat
    15541 agagagaaaa agtagtgaga atggtacaga agtattaatt actataccta cacaaattaa
    15601 tatttaaata ataactcatc tacaaagtag tagttttaat tgagcaaaga ggcttaaggt
    15661 tattaataga ttaagttaat tgaatgtatt tataaaaatt ataagtatat attcaattta
    15721 acaggtattt attataactt taagcctctt tgcttgttta agacatactt aaattttagg
    15781 aggtgagaaa atgagagaag agttattaag tgttggtatt gacattggaa caagtactac
    15841 tcagttaatt ttttctaaat taatagtaga aaatactgaa tcaaattttt cggtacctag
    15901 aataagtata gtagacaaag aaattattta taaaagcaaa gtttatttca cccctcttat
    15961 ttcaaataat gaaataaatg gtcaacaaat aaaagatata gttgaaaatg aatataaaaa
    16021 agcaaatata tctaaagaag atatacatac aggagcagta ataataactg gtgaaacagc
    16081 tagaaaagaa aatgcaaatg atgtacttca tactttgagt ggatttgctg gggacttcgt
    16141 tgttgcaaca gcaggacctg atttagaaag tataatatca ggaaaaggag ctggagctca
    16201 tatttattca aaagaacatt caactacaat tgtaaatata gacataggtg ggggaactag
    16261 taacatagcc tcatttaaaa ggggagaagt aaccgatact gggtgtttgg atataggtgg
    16321 aaggcttgta aagatagata aaataagtaa aaaaataact tatatatcac ctaaaataga
    16381 taaaatcata aaagataata atttaaatct tcaagtaggc acaatggcta ctgtagaaaa
    16441 tacaaagcca atagtggata taatggttga cttattaatg gaaagcatag gattaaagaa
    16501 acagacacct ttgtttgatt ttataataac caataaatca ataagtatag atgaaaatat
    16561 taaaaatgtt tcattctctg gtggagtagc tgattatatt tactatgatg gagaaataaa
    16621 agattatttt gaatatggtg acttgggaat tttacttggg caagcaataa aaaattgtga
    16681 ataccttaaa aaagtaaatg tagttaaatc tatggaaaca ataagagcca cagtggttgg
    16741 agcaggttct catactactg aagtaagtgg aagtacaata acttacacta gcgaaaagtt
    16801 tccacttaag aacttgccag ttttaaaatt atcaattgat gaagaaacag gcacttcaaa
    16861 acaaatcagt gaagctataa aaaataaact taattggttt aaactagaaa atgatttaca
    16921 gaaagttgct atagccatga taggaaagaa aaatccaacc tttttagata tacaagagta
    16981 tgctaaaggg tttgtagatg gaatgcaaca aatcatagaa aatgatttac cttttatagt
    17041 aattgttgaa aatgatatgg caaaagtgtt aggtcaagca atatatgcat tattaaatta
    17101 tgaaaaagaa attgtttgta tagacagtgt caaggttgaa aatggagact atatcgacat
    17161 tggaaaacct atagttgaag gtaaagtttt acctgtggta ataaaaacat tagtttttaa
    17221 ttaatagaag gaggtaagta aatgatttta aatacaaaat tatttggacg tacttatcag
    17281 tttaaaggct tatatgatgt aatggctaaa gctaatgaag aaaaatcagg agataaatta
    17341 gcaggtcttg cagcagaatc agctgaagaa agaatagcag caaaagttgt tttatcacac
    17401 ataaaattgg aggatataag aaataatcct gcagtggcat atgaagaaga tgaagtaaca
    17461 agaataatac aagatgctgt aaatgaaaaa gtttatgaag aaataaaaaa ttggacagtc
    17521 tctgaactta gagaatggct attggattca aatactacaa gtcttgatat aagaagaata
    17581 tcaagaggat taacttctga aatgatagca gcagttgcaa aattaatgtc aaatatggac
    17641 ttagtttatg cagctaaaaa gataaaggtt actgctcact gtaatacaac tataggagaa
    17701 caaggaacat tatcagcaag acttcaacct aatcatccaa cagacgaccc agatggaata
    17761 cttgcatcac ttcttgaagg tttaactttt ggtataggag atgcagtact tggattaaat
    17821 ccagttgatg actctgttga gagtgttact aaagtattaa aaagatttga agaagtaaaa
    17881 caaaaatatc aaataccaac tcaaacatgt gtattggcac acgttactac acaaatggaa
    17941 gcaataagaa atggtgctcc tgcagattta atattccaat ctatagcagg ttctgaaaaa
    18001 ggaaatgaag catttggatt taatggcgac actatagaag aagctagaca gttagcctta
    18061 aaacacggta cagctacagg acctaacgtt atgtattttg aaactggaca aggttcagaa
    18121 ttatcttcag aagcacatca tggaatagac caagttacta tggaagctag atgctatgga
    18181 tttgctaaaa agtatgaacc attcttagta aatacagttg ttgggtttat aggacctgag
    18241 tacttatatg atgctagaga agttgtaaga gcaggacttg aagaccactt tatgggtaaa
    18301 ctacatggct taccaatggg tgttgatgtt tgttacacta accatatgaa ggctgaccaa
    18361 aatgatatgg aaaacttagc catactatta acaacagcag gatgtacgta ttttatggga
    18421 atacctcatg gagatgatgt tatgcttaac tatcaaacaa caggatacca tgaaacagca
    18481 tcacttagag aaatgtttgg actaacagca ataaaagaat ttgatgcttg gatggaaaag
    18541 atgggattct ctaaagatgg taagttaaca gagttggcgg gagacgcatc tgtattatta
    18601 ggataggagg tatttaatat gaatgagaag gatttaaaag cattggtaga acaattagtt
    18661 ggacaaatgg ttggagaatt agatactaat gttgtgtctg agactgttaa aaaggcaact
    18721 gaagttgtag tagacaacaa tgcttgtata gatgatataa cagaagttga tataagaaag
    18781 caattactag taaaaaatcc taaggatgca gaagcttatt tagatatgaa agcaaaaaca
    18841 cctgcaagat taggtatagg aagagctggt actagatata aaacagaaac agttttaaga
    18901 tttagagcag accatgctgc tgcacaagat gctgtattct cttatgtaga tgaagaattt
    18961 ataaaagaaa ataatatgtt tgcagtggaa actttatgta aagataaaga tgaatactta
    19021 acaagaccag atttgggaag aaaattttct ccagaaacta taaataatat aaaatctaaa
    19081 tttggtacaa atcaaaaggt tttaatatta gttggagatg ggcttagctc agctgcaata
    19141 gaggcaaatt taaaagactg tgtgccagct ataaaacagg ggctaaaaat gtatggtata
    19201 gattcaagtg aaattttatt tgttaaacat tgtagagttg gtgctatgga ccacttaggt
    19261 gaagaattag gttgtgaagt tatatgtatg ttggttggag aaagaccagg attagttact
    19321 gctgaatcaa tgtcagcata tatagcttat aagccatata taggaatggc agaagcaaaa
    19381 agaacagtta tatcaaatat tcacaaaggt ggaacaactg cagttgaagc aggtgctcat
    19441 atagctgaac ttataaaaac tatgttagat aaaaaagctt caggaataga ccttaaataa
    19501 agtcgaggga gtgtagaata tgaaaaatga tgtaattcgt cctaatatac taggaattaa
    19561 aataatttca aatataagtc cagaaatggc acaaaaacta gaattaaaat cacatcacaa
    19621 aagcttaggt ttaataactg cagactgtga tgatgttacg tatacagctc ttgacgaagc
    19681 aactaaagct tcagaagttg atgttgtata tgcaaaatca atgtatgcag gtgctgcaaa
    19741 tgcttcaaca aaacttgctg gtgaagtatt aggaataata gcaggtccaa gcccagcaga
    19801 agtaagaagt ggtctaaatg cagtagttga ttttctagag tatggagcaa cttttataag
    19861 tgcaaatgat gatgattcaa tagcatacta tgctcactgc gtatcaagaa caggtacata
    19921 cctttcagag gtagcaggaa taagagaagg tgaagcatta gcttatttag tagcaccacc
    19981 acttgaagca atgtatgcac tagatgcagc aatgaaagct gcagatgtta agatgtgtga
    20041 attatttgca cctccaactg aaacaaactt tggtggagca ttacttacag gttctcaatc
    20101 ggcttgtaaa gcagcatgtg atgcatttgc agaagcagta aaatcagttg cagacaatcc
    20161 aacaggattt taaggatagt tagatggatg ctataggctt aatagaagta ataggatatg
    20221 ttgctgcaat agaagcttct gatgcttgtt taaaatctgc aaatgttaat attgtaagag
    20281 ttgataaggt tggagcaggt attgttactt taacaatatg tggagatgtc ggtgctgtaa
    20341 aagctgcttt agaagcaggt gaaatagcag cttccagagt tggtacatta agaacatcac
    20401 atattatacc gagaatccat aatgaagtaa caaatgcttt attcaaaact aaagaaccta
    20461 aagtctgtga agtttcagaa gataaagaaa aaaactttga gattacattg gaaatagctg
    20521 ataatgtatc gaaaaattta tcagaaaaag aaaatgtaat tttagatgtc aatgaaaaca
    20581 gtggagatac tgaaaattta tatagtgata tagataaaac aaacaaaaaa attgaaacag
    20641 ttgatgatag tttaacagaa gctaatgagg aaaaaataga aaacactaat gataaagaag
    20701 tattggaaca agttaaaata gaaaattccg attttgatag agatgaccta tcaaagaaca
    20761 gtgtaaaaaa acttaaagct atggctaaga aattagattc ttcaataact tataaagact
    20821 taaatgttct taaaaaagaa gaattaataa acttagttaa aaagcttagt agaagggata
    20881 gatagttatg acacttttag ataaagattt agtatctata caagaagtaa gaactcttgt
    20941 aagaagatct aaagtggctc aagagaaact ttctaaaatg agtcaagagc aaatagataa
    21001 aatagtaaaa gctgttgtag atgcagcatt taataatgct actaaattag ctaaaatggc
    21061 aaatgaagaa actggatttg gaaaatggga agataaagta attaaaaata cttttgcatc
    21121 tatgggagtt tatgaaactt taaagaacac aaaaactatt ggaataattg gtgaagataa
    21181 agaagccaat acgatagata tagctacacc agttggagtt gttgcaggtc ttataccttc
    21241 aacaaaccca acatcaacag taatatacaa aactataata tctctaaaag caggaaatac
    21301 tatagttttc tctccacatc caaatgcaaa aaactgcata cttgaaacag ttaatataat
    21361 aagtgatgct gctataaagg ctggagctcc agaggattca atattatgta taactaatcc
    21421 aagtatagtt ggaaccaatg aattgatgaa acataaagat acaaacttaa tactagcaac
    21481 tggaggagaa gcaatggtta gagctgctta ctcttcagga acaccagcta ttggagttgg
    21541 accaggaaat ggaccagcat ttatagataa atcagcaaat gttaaattag caatcaaaag
    21601 aataatagat tctaaaactt ttgataatgg tgttatatgt gcatcagaac aatctatagt
    21661 aatagaaaaa tctatggaag aaactgtttt aaaagagctt aaaaatcaag gtgcatatgt
    21721 tttaaatgag gaggaaagtg aaagattagc aaaattcata ttaagagcta atggtactat
    21781 gaatccacaa atagttggta aaagtgttga ggtaatagca aatttagcag acttaacaaa
    21841 tgtacctaaa aatacaaaag ttctagttgc aagagaaaat aaagttggtc acaatgtagc
    21901 ctattcaaga gaaaaactta caccaatact agcgttctat gttgaagaaa atgtagaagc
    21961 tattatgaac agatgcagag aaatattgtt aaatgaaggt gcaggtcata cattctcaat
    22021 gcatgtaaat gatgaagaat tggtaaaaag atttgccctt aatataccag tatcaagaat
    22081 acttgtgaat actccttcag cacttggagg aataggtgca tcaacaaacc ttgtaccagc
    22141 actaacactt ggttgtggtg caataggtgg aagttcaact tcaaacaata taggaccact
    22201 tgatttaata aatgttaaaa gagtagctta tggaatcaaa gaaatagaag aacttggaag
    22261 agataaatct caagttgaaa aatcaatgtt tgactcaatg gataaagaag aattaataaa
    22321 tcttcttgtt caaaaactag tcaaagaact agcataacta aaataaacaa tgaaacttat
    22381 ggctatcgct ataaatttct aaaaatataa actataaaaa actataaaat taaatttttg
    22441 gaggataaga ttatggcaag tgcaaacgca ttaggaatga tagaaactaa aggattagta
    22501 ggagcaatag aagcagcaga tgcaatggtt aaagctgcaa atgtacaatt agtaggaaaa
    22561 gagcaagtag gtggaggatt agttactgta atggtaagag gagatgttgg agctgttaaa
    22621 gctgctacag atgcaggagc tgccgctgcc gagagagttg gagaactaat atcagttcat
    22681 gttataccaa gaccacactt tgaagtagat gctatattac caaaggtatc agctgagtaa
    22741 tataagaata attataaaat ttaaattttg attgaaaaag aggctatctc aaaaatgagt
    22801 cttactaata aactcaatgt attttgaggt agtctttttt atacatctaa taggagatgt
    22861 gataaaatgg gtgttttaac agaaaacgaa gtaaaaagaa aaattggaaa aaacaaaaat
    22921 aatataactg aattttatat agaaaaaggt caaataataa ctccttctgc aaaatcttat
    22981 ttagctgaga gaagtattga gcttaaatat gttgataaac tttcagatgt aaacaatgaa
    23041 cctaaagaag ttgtaaaaga agtagttaaa gagaaaaaat ataaatatac tactgtattt
    23101 ggcgccaaat tggatgaaaa accagaacat atgactcaat taaatggaaa tcttttagtt
    23161 tttaaagacc ataaaagaat aatattgaga ggaaaaatag attctcttga atcagaaata
    23221 ctaaaggtac agatactttg tcaaaaacta gatatgccaa agcttgttaa ggaattgcaa
    23281 gaagtattag actttgtaag aaatataatg agatgtgaag ttttagatga aaagctaggg
    23341 gactttaaac ttttaggatt aactcctcaa gaacttagag aacaatctca ctatcctaaa
    23401 aaatactttg gtataggaca tgagttccca agttatgaaa tgggagaagt agtagtagct
    23461 ataaatagta taagaagtaa tacacgagag gttgaacttg cagcctatga agcatttaag
    23521 ggtgagtatg gaaatgttca cagagaagat ttaataaagg cacttaatag attatcgtca
    23581 gtattttgga taatgatata taaggttaga actaataagt ataagtagtt ggaggacata
    23641 atggagaatc tattaaatga aatagtggaa gaagtaataa ctagagttaa aaaagaagca
    23701 tttatagaag ttgaagcatc aggaagacac gttcacttaa gtagagaaga tgtcgataaa
    23761 ttatttggag aaagatatac tcttacaaaa ctaaaagacc tatctcaacc aggtcaatat
    23821 gcatgtaagg aaagagttac aataactggt cctaaaggaa gtataaaaaa tgtagttgta
    23881 cttggaccat gtagaaatga aactcaggta gaaatatctt taacagatgg ttcaactctt
    23941 ggactaaaag ctcctataaa acaaagtgga gacttagaag gtactttaag tattaaaata
    24001 tcaacacaat atggtgaagt tgaacttgat aaggggctaa tggttgctaa aagacatata
    24061 cacatgactc ctaaagatgc tgaaaaattc aatgtatgtg ataaagagat agttgaagct
    24121 aaggtgatgg gtcaaagatc tttaacattt gatgatgtag taataagagt gagtgacagt
    24181 tttaaaactt atatgcatat agactatgat gaagccaatg catgtggtta ttcaaaagga
    24241 actgttgcta aaataataaa gaaagcataa atttaaccta ctatataaat tacctctagg
    24301 gaggatttaa tatgaattat gataaccttg tagatttaat aacggaagaa atctataaaa
    24361 aaataaatag taatgaaata aaaatatcta ataaacctaa agctgtgatt gtatttgaac
    24421 aagataacaa taaatttaac ttattaaaag atgaatttga aatagtagaa tttgataaaa
    24481 gtataaggga atgtgaaata gtgatagttt caagactttg tatgagggga ctatcgaata
    24541 tagcacttgg aaattcaaca agtgatgaag aaagatttat actaaaaatg attatgaagg
    24601 gcaaaaaagt atatgtccta gatgaaggaa ttgaatacaa aaaatataag gatactgcac
    24661 caaaagcatt atataagaaa tttatgtcat ttgaagatga aatatgtaaa tttggtgtag
    24721 aaattataaa agacttaaat gttataacta aaaataagtt aaatataaag aaagaactag
    24781 aaagtactag aggttctaaa aaaaaagata caaaagtttt agataaagat gttttaaatt
    24841 taaccagtgt agaaagtgta aaaatagata atgaatttag tttagattta aggaataaaa
    24901 aacttatatc ggaagctgac ttaagaaaac caactataaa tggagttaaa aacatattag
    24961 ttaataaaaa aagtataata acaccattag ctgtagattt tatgagaata caccatctca
    25021 aactaaagaa actgtaagga ggtaatttta tggatatagg aaaagttgta ggtaatgttt
    25081 gggctacaag aaaagatgaa aaattatgtg gccaaaaact tttagttgta aaaattttaa
    25141 aaactaaaga cacatacaag gaaggcttca tggttgcagc cgataatgca ggagcaggaa
    25201 atggagactt agttcttata tccaaaggtt cctcagctag agagtcgatt gaaaaatctc
    25261 atgttccagt agatgcaact atagttggag tagtcgattc atttgaggtg tttgatgaat
    25321 aaaagtatag gagccataga gtttaaaagc attgctaaag gtatagaagt atccaatgaa
    25381 atgataaaga aatcttcagt tgatgtattg tacttaaaaa gtatttgccc aggtaaattt
    25441 ctaataatag taggtggaga aacttcttat ataaatgaat gtgtagatta tggaataaaa
    25501 cttggagaag gatatattgt agataacttt gtaataaatg caatttcaca agaaatatta
    25561 gatggattta aaaataagta tcaaaagcta gatagtatag tctctatagg cgttgttgaa
    25621 agtagtaaag tatgcgctgg aataaaaatg cttgataaga ctttaaaatc cggtgactta
    25681 gtattagtaa aacttcaatt atcttttgct ataggtggca aattagtata tatagtagca
    25741 ggagatttaa gtagtttaga atatgcatta aaagaaagtg aaaatgtagt aagagaaaaa
    25801 gaagtaatat ataagactgt aataccatca gtagacagtc aaataataaa aagtctaata
    25861 aaataatggg gggaattatg agtataaacg aaattataat ctatgtaatg gtagtcttca
    25921 tggtacttgg agctatagat aaatgtatag gtaacaaatt tggtcttgga gaacaatttg
    25981 aagaaggtat aatggcaatg gggtcacttg cagttgcaat ggttggtgtt atatgtttag
    26041 caccagttct tgctgatgta ttaagaccaa ttgtagtacc agtatttaat atacttggag
    26101 ctgacccagc tatgtttgct ggaagtttat tagctaatga tatgggtggt gcgccacttg
    26161 cacttgaact tgcgaaagac cctaatgctg ggttatttgg tggattaata gtaggtgcta
    26221 tgatgggacc tacaatagta tttataatac cagttgcgct tggaataatt gaaaaagaag
    26281 accaaaaatt cttagcaaca ggaatacttg caggtataat aacaatacct attggagcat
    26341 tcgttggtgg attagttgca ggattcgagg taatgatggt tcttagaaac ttagtgccaa
    26401 taataatatt tgcagtagtt atagcacttg gattattaaa attcgaaaat gctatgataa
    26461 aaggattcac atactttgga aaaggtgtag taatagttat aactttagga cttgctgctg
    26521 ccatagttga agctttaaca ggaatagttg taatacctgg aatgaaacca atcagtgaag
    26581 gtatagaaat agttggagat atagcaatag ttttagcagg agcattccca ttagtgtttg
    26641 ttataacaaa ggtatttaat aaaccattaa tggctttagg aaaagttctt ggaatgaatg
    26701 atgtatcagc agcaggcctt gttgcaagtt tagctaactg tataccaatg tttggtatga
    26761 tgaaagatat ggacaatagg ggaaaaataa taaatgtagc attctctgta tcagcagcat
    26821 tcgtatttgg agaccactta ggatttacgg caggatttaa ttctaatatg ataacaccaa
    26881 tgatagttgc taaattagtt ggtggtataa cagcagtatt gttagctatg gttatcgcaa
    26941 ataagacact aaagaaggag aatgtataag atggatatat caaatataga taaaaattta
    27001 attgaaacat tagtaagaca aataatagaa gaaaaaataa gtggaactaa agacacagta
    27061 gattttgtta gaaataaaga cattagtgga ataacttcta taaagttacc aactgtaaaa
    27121 gttagtgaat ctgatagatt agatacaggg aatccaagtg atgtagttta cacaaaagat
    27181 ttatttacat tagaggaaag cccaagatta ggttgtggta tgatggaaat gaaagaaaca
    27241 acttttgatt ggactctaaa ctatgatgaa atagattatg taatagatgg aactttagat
    27301 ataataatag atggaagaaa ggtttctgct tcttcaggtg aattaatatt cataccaaaa
    27361 ggaagtaaga tacaattttc tgtacctgat tatgctagat ttatatatgt aacatatccg
    27421 gcagattggg catctcaaaa ttaataaaaa tcaagttaag ttaataataa tcaaataaat
    27481 aatttaaaat acaaactctt aaataagcac acatatttaa aaaagctatg aattaaaatt
    27541 catagctttt tttataaata atgttatatt ttagacgtat tttttctata tttactggga
    27601 gacatattat attttttatt aaataatcta ataaaatgct ctacatttgg ataaccaata
    27661 ctattttact gataatcttt tccttatcac taatcttaca ttttaaaata ttatttttac
    27721 tcgtaatatt atcataagaa tttgtttctt atttaaaact aattagaatt ataaagttta
    27781 ttgccatgtt attaataaaa taaatccagt taaatcgagt gctagttaaa gtaataagaa
    27841 acttttacaa tggtttcctt tatcgattga aataaaagtt aatatggtat aatatctaaa
    27901 gagagaatct tatcaattag aatatgcata gaagcaagtg gcttaaaagg agacgttata
    27961 tggaagagat agtaaagaaa gaacaagcaa gtatgaatga tatttttttg tatcttaaag
    28021 ctaaatcaaa ctcattatat aaatttgtaa tattatataa tgagtttatc aataaaccta
    28081 aagattatgg aactggggaa cttataaaca tggttgaagt acatatgcta acagcgattg
    28141 aagaaaatcc aggaataagt ccaactcagt tagctaaaat atggaacaga actaaaggtg
    28201 caatatctca aactatatct aaattggaga aaaagggtta tataactaaa gaaaagaaag
    28261 aaggaaatgc aaaaactata ttactatatc caaccacatc aggagtagag ttaagtaagg
    28321 cacataaact atatgacacc atagatgtta caagaacatt tgaatactta atcaaggagt
    28381 gtacattaga ggagatagat agtttttata aagtaattga tttgtttatt aatttattag
    28441 aagaatctcc gcaggaaaat aatgattctg aaaaagtgta gctaagattg aagttgaata
    28501 aagagaaata gatattatag aagtagtact taagtacaca ttatataaaa atataatagg
    28561 tatatgatta aaaagtttag tggtttaaag tttaaccact aaactttttt aatgtctata
    28621 atctactatt atttaattta ctaaaaataa aagaactcat tatattaaga taaaaataga
    28681 aaaataaata gaaaaatact ataatatatc agcttaatac agaaaaaagt taaaaaattt
    28741 gagaaaaata aaaatgaaaa cgttttatat tgacatagag aaaaagtagt gataccattt
    28801 aaatatagtt tagtagctaa actatattta aattataata actattttag ggggatgtta
    28861 tgaatttaaa acaattaagt gaaaaatacg aagaatatgt aattcaaatg agaagagaat
    28921 ttcataagta tccagaatta tcttttaaag aaataagaac aacccaaaga ataagggaag
    28981 aattacaaaa aataggggtc ccttttgtat ctattgaacc aaatatcgta atagctacta
    29041 tacaaggcaa aaatgaaggt aagacaattg caattagagg agatatagat gcgcttccaa
    29101 tgcaagaaga ttcagatgtt gaatacaaat cagaatatga tggaataatg cattcttgtg
    29161 gacatgatgc tcatggagct atgttactag gacttgcaca tatgctaaat gatataagaa
    29221 aagatttaaa aggtagagta ttattaattt ttcaagcagc tgaagaagtt ggtggcggac
    29281 acaaggagat aatacagtac tttaaggaaa atggaggacc agatagatta atagctactc
    29341 atgtttggtc cgatattgaa gctggtaaaa tttctgttga accaggacct cgtatggctg
    29401 gtgggtctgg ttggagaata gacataacag gtagaggagg tcatggttct agaccagatc
    29461 aaagcataga tcctataaag ccagcttgtg atatagtttt aaaagtatct tctataccag
    29521 ttaatcatat aagtgtacta gaacaatgtg ttgtacatgc atgtgcaata catggaggaa
    29581 ctacaatagg aaatgttatt ccaaacaatg cagaggtaat aggtggatat agatatttta
    29641 ctgaagaatc aggcaaaaaa gtgtttgaca ttataaagca aatgagtaat ggtgttggac
    29701 aagtttatgg agtggatgtg aaggttactc atactggagg cataggacct gttatcaatg
    29761 atgaagaagc agttaagatg gctaaagaaa tagtttctaa tatagatgga ttggagttag
    29821 attcttatga gccaatatgt gcatcagatt gttatggtga aatattaaaa gagtattctg
    29881 gtttttattg ttatctaggg gtaggtaaca aagaaaaagg aataatatat gcacaacacc
    29941 atcctaagta caatatagat gaagatacat taaagttagg ttgcgaattt atggctaaat
    30001 atgcagttga atttttaaat aaaaataata actaaaataa atcgaatttt ataaatgggg
    30061 ggatgaaaat gcaaggtgca ttaaaggaaa ataagaaaaa aagcggtgga ggatttttag
    30121 gatggattga aagagttggg aataaaatgc cacatcctct agcattattt acatatatta
    30181 cattgatagt agtagcatta tctgcaatag caagcttttt aggtttttca gcaacaagtc
    30241 ctgctacagg aaagttaata aaagttatga acttaataag tgcaaaaggt ttagtactat
    30301 ttatgcaaga gtttgttaag aacttccaaa acttccctgt gcttggagta gttttagtta
    30361 tggcagttgc cacaggagtt tgtgaaaaga caggattttt ttcaactgcc ataaaaatga
    30421 gtttatcaaa agttaagggg aatgcggttg tatttataat agcttttata ggagtatttg
    30481 ctaatcaagc tggtgatgct gcttttgtat tagtaccaac tcttgcagga gcaatatttt
    30541 atggattaaa tagaaatcca ttagctggta tattctgtgg atttgcatca gttggtggtg
    30601 gatttgcaac agctgtaatt cctgggggat gggacgttac gctaacgcca ataactgtat
    30661 cagctgctca aactattcaa cctgcatttg atatgacatt attagctagt tattatgcac
    30721 tatttgtatc tgcgtttata gtagctacag tatcaacaat agtaacggtt aaatttatag
    30781 aacctaaatt aggtaagtat actggtaaac cagaaggtat agatgataat caaggttttg
    30841 aggtcacaaa agaacaagca aaagcagcaa agttagcagg ttttacagtt ttagcttatg
    30901 tagcgttatt aattgctttg tgtataccag ctaatagttt tttaagaagt ccagaaggtt
    30961 ctttaatatt tggagcacca cttatgtctt gcttacagtt ttttatagta atactattct
    31021 tcttgccagg gattgtgtat ggaatgaggg ttggtaaaat caagactgta aaagacttaa
    31081 atgatatatt atgtcaatca atggcagcaa tggcaccgtt tatcgtactt gcaatagtta
    31141 taggtcaatt cttaacgcta ttctcagtgt ctaatttagc acaggtacta gctataaaag
    31201 gtggagcatt actcaactca ttgccactac caccacaagc tataatactg ctattcttag
    31261 tactagtagc gtttataaat atatttgtta taagcggaag tacaaaatgg atgatatttg
    31321 gaccagtatt tgttccaatg ttaatgcaat taaacataca tccagcattt actcagtttg
    31381 tatatagatt aggagattct gtaaccaatc atttatcacc attaaatgca ttctttataa
    31441 tacttttagc aacagtccaa aaatacgata aaaatgcagg tatgggaact atattctcag
    31501 ctatgatacc ttacactata ggttactttg tagtacttgc agttttagta gtagtttgga
    31561 tgacaatagg tataccagta ggaataggtg gacaagtttg gttataaaac atatttaata
    31621 attttgggag gaaagaaaat ggaaacaact gaaataagat acaattttga tgaagtaata
    31681 gatagaagtg gaacaaactg tcttaaatat gatcttaaga gtcaatttat gccagaatct
    31741 ccagaagatg cactaccact atgggtagca gatatggatt ttgcatgtgc ccctgagata
    31801 attaatgcaa tgcatgaaag aatagacaga aaaatatttg gatatagctc tcaacaaact
    31861 gatgattatt atgaagctgt atgtggatgg tttaagagaa gatttgattg gaaaatagaa
    31921 aaagagaata tattctttag cccaggggtt gtgcctgcgt taggattttt cacacaaata
    31981 ttaacacaaa agggtgatgg tatcataata gaaaagcctg tatactatcc ttttgaagat
    32041 aaaataacta acaatgaaag aaaagtagtt aatagtcctt taaagtttga agatggtcat
    32101 tattctatgg attatgatgg atttgaagaa ttagctaaaa atgaaaataa taaggtgttt
    32161 atattatgta gtcctcataa tccagttggt agagtatgga aaaaagaaga gcttagaaaa
    32221 atagtagaca tatgtaagaa atatgatttg tggataatat ctgatgaaat acattgtgat
    32281 attattcgta aaggtgaaaa gcatatacca ttagaagtag catgtcctga ttataaagat
    32341 agaataataa cttgtacagc tccaagtaag agttttaact tagctggtat gcaattatca
    32401 aatatagtaa tcaataataa ggaattacaa gaaaaatgga tgcttgaaac agattctaaa
    32461 ttagctatat tcctacctaa tccattcgca atagttgcta ctaaagttgc ttataatgat
    32521 tgtgaagatt ggatgaatca agtaaatgag tatatagatg gtaatattaa atatgtagaa
    32581 gattttgtta atgagtatat gcctaaagta aagataataa aatctcaagg aacttactta
    32641 gtttggttag actttagagg gtatggatta gatgcaaaac agcttgaata tgtaatgctt
    32701 aacaaggcga aggtattgct agatgaagga tacatattcg gagaagaagg agcaggattt
    32761 gaaagaataa atgttgcttc acctagaaca attatagaag agtgtatgaa tagaataaaa
    32821 tgtgcatttg aaggattata atagtaatat ctaaataata tatttgtgta ttttaaaaaa
    32881 agactatgct aaaaaaatat ttcaaagcat agtcttttaa agttttatga tgagtttaaa
    32941 aaatttcaaa acgttactat tcacttatat gtttatatta gaaataattc cacaatatac
    33001 tcattttcta aagttgcact tttatatacc ttgcttcacc aatatcatgt gtaaccaaaa
    33061 tagtagactt attctcattc ttaataatac tataaacatc atcacaaact aataatctag
    33121 tctgataatc aagagctgaa aaaggttcat ctaaaagtag aatatctgga tttacagaaa
    33181 gtgttctaat aagcgcaact ctttgtctca tacctccaga taattctttt ggatacatac
    33241 ttctaaaatc ccataatcca taagtcttta aaaggctttc aactctatct aaagattttt
    33301 tatctttttt gccttgaatt tccaatccaa tagttatatt atccattata tttctccatt
    33361 caagcaaatt atctttttga aacatatatc caatatttcc agtaatcttt atatcaccac
    33421 tagtcggttt taaaagatta gtaagaatat ttaatattgt agatttacca cttccagaag
    33481 gaccaagcaa agttaatatt tctccttctt tcaagttaaa attgacatcc tttaatactt
    33541 caagctcgcc ttccttagtg tagaagttca tatttatatt tttaacttct actatgttgt
    33601 cattcattat gccaccccct ataactttta gctcgtatat attatgttat taacaatatg
    33661 acctatttgt accatttttc ataaatataa tataaatatt ttgtttattt acgtattttc
    33721 ttagtaactt gtacgtataa attatagata aataaattat aaggtagggg ataaatttga
    33781 gtaacataat aacgaattta atagagtcaa tatttactaa tccaataact atattgtttt
    33841 tacttgcaat aactgtgtat tctgttttta aaataataga aaataacaga gtatattcat
    33901 ttgtaagttc aaaattagac gaaatcaata gagactataa gacaagtgag ttttataaaa
    33961 aggtaaggga tgactactat acatatagta aagaaaatcc ttatgcagac gtaaatataa
    34021 cttcatttat agaggaagtt gtatctgatt taaagcataa taatttgcca ttgttagaaa
    34081 aaattagaag tataaaaaat tcatcttcaa taagtatatt acttggggtt ttaggtacgt
    34141 ttgttggtct ttctacaatg cttttatgtg ttgatactaa agacattata aattcacttc
    34201 cttcaactat tagtagtatg caaactgcat ttacaactag tatatttgga gttgtatttt
    34261 ctctaattat aggttacttt actaagataa aagattgtga acatgttttg atacaaataa
    34321 tgttaaaatc tgaaaattta ctaacatctg aaataactca ttttaagtca gaaagaatgg
    34381 atttaaaagt agaagaggta aaaaatacaa taaaacaaat aagtaagtct atagaagcta
    34441 ttgaaaaatt tgataaaata tctaaagatt taaatgactt taatgatgaa tttatcagtg
    34501 gtatagaagc tttaaaaagc cttttagaag gttctcaaag ttctattaag acatttgacc
    34561 agagcgtaag aaaattagat aaacagttta atattttaaa tataaaattt gtaaagcttt
    34621 ttgataaata tgataatcaa gataatataa ataaagagat tttatttgat ataaaagaat
    34681 cttcaaaaaa tatatacaat gccactgaaa gccaattcaa aattagagat tatataaaaa
    34741 atataaatgc aggatttgct ctatatgaaa gaagtgctca agacttatta actaaattaa
    34801 tgacccatga aaataaaatc tctcaaaatc aaaaaatcct tttagatgaa aagtttacct
    34861 tagatgactc aataaaaaat ctctcaagta ttattgaaaa cttttctaat gacctacaag
    34921 ttaaactgga tatgatgttt gaaaattctt tagatattca agataagtta gatgttatgt
    34981 ttaataactc ttttatgaat gatgaagttc ctcttgatag tgaagaatta tttaaggatg
    35041 atgttaataa tgtatttaat ccttttagtg aagaaattta tgagatagag gataaagaaa
    35101 taaaggtcat aggtgaggat gagttaaatg aataataaat atagaagaac tgttaacaga
    35161 gaatttgaaa agagtagctt ttggccaaca tttacagatt tattatctac tgtattgatg
    35221 gttgtaattc ttatcttatt tagctcagaa agtatttcag gttctgttga acaagattta
    35281 gctaagaatg tcaatgcttc tgttgaagaa acttttaaaa aaagtggaat acctgtgaag
    35341 gtagataaat ctaatgggca agtaactttt ggtgaaaaaa ccatgtttga tgtagatagt
    35401 gatgttttaa aaccagaggc taaagaaatg ctaaaaatgt ttgttccaaa gtacatagag
    35461 actatttata aagattatgg tgactatata tccaaaatag ttatagaagg tcatacagat
    35521 gatgtgggaa gttatatata caacttagat ttatcgcaaa gaagagcata tagcgttgct
    35581 aaatttatag tgggagatga aataggtgac tataaataca aagataaagt gactaagcat
    35641 attattgcaa taggaaggtc aaaagctgaa ctcatcaaaa atggtgacaa tagcgtgaat
    35701 agagatgcat ctagaagagt agaactaaaa tacgaaataa atattaatca aaataaataa
    35761 ttaaattttt ataaaaaaaa agaaggcagt tggtgtgagc tttaagcctt ctttaaggaa
    35821 aaagtttatt tatttttgtt gatacttaac atattatagt ttttagaagc agtttgcaat
    35881 atattaaacg taaatggtcg tttaaaatgc ataactggat actactgatg taaaatattt
    35941 cattttatat aatataaaaa ttatacagtg tcaattttct atattattgt actataatct
    36001 attatttaaa atattatgta aaaaaatatc ataaaaacgc atatcatgtt ataattacta
    36061 gagtattatg aatatggagg cgtagtttgt gatagaaata agtataaaaa ataaaaaaaa
    36121 ttctaaaatt aaaactttga tacttttatt gatagtatct atatcgattt atttaactgg
    36181 ttgtactaca tcagataatg gaaataatca atcaaataat aatgattctt tgtctagtag
    36241 tatatcaagt gataagaatg taaaaattca ttttattgac actggaaata gtgatgcaat
    36301 attgattcaa gatggtaaaa cttttacatt gatagatggg ggagataatg atgatgaggg
    36361 attaatggta gattacctaa acaatcaagg tgtaaaagat attaaatatc ttatagctac
    36421 ccattctcat gcagaccatt taggtggact tgattctgtt gtcaaaaact ttaatataga
    36481 aaatgttttt gtatcgaatg gttcagcaga aacaaagagt tatagggact ttataaatgc
    36541 tttagcaaat aagggtttat ccccaagtgt accgcttgaa aataataagt tctatttaga
    36601 agattcttat tttgaagttc taaatacaaa tgggggagat actactaatg aacaatctct
    36661 agtccttgta tatactaatg ggaatgataa agttttattt acaggtgatg ctgaagaagg
    36721 taccgaaaaa gaaatactac ctaagcttga gaaagtagac cttttaaaag tagctcatca
    36781 tggttctcgt agtagttcat ctcaagagtt tttagataaa gtaaatcctg aatatgcggt
    36841 tcttttagta ggcaagggta attcatatgg tcatccacat caggagacaa tgaataaatt
    36901 aaaaaaaatg ggagtaaaag tacatagaag tgatgagtgt tcagatataa tatttgaatc
    36961 aacaggtgat ggagtattta caagttgtaa agatggaagt tataataaag gcgttagaga
    37021 agatggaaac tctggaaaat caaacagtaa tacaagtaaa aatgattctt ttaaaaataa
    37081 acaaaataca tccaattcag aagaaatagt gtattttact ccaaaaggta agagttatca
    37141 ctctacaaaa aattgtagtg ggctttcaag aagtaaaaaa atattatcag gcacaattgc
    37201 agaaagtaag aaaaatgacc catgtgatat atgttatgga aaataacaaa aaaacaacca
    37261 atttatctat aaagcttctg aactttaaat cattcaaaaa ataaaataaa tgcataaaaa
    37321 aataagctat aatcaataga taacacattt tatctatacg aatagcttat ttttttaagt
    37381 attttttaat agctatttaa taactctaaa tacaccagtt acaataccta caatttcaac
    37441 ttctgagtca ttgagcataa ttggttccat aaaatcattt tctggctgaa gtcttattaa
    37501 atttccttct ttaaaaaacc tcttaactgt agcagattca ccatttataa gtgcaactac
    37561 tatctgagaa tttaaggctg tatttttttt gtctactaca acgtaatctt catctaaaat
    37621 tcctgcatta atcatacttt cgccttttac tcttaataca aaattgttac tgccctttac
    37681 taaactggca gggaatggaa tgtattcctc tatgttttgt tgagctaaaa tgggttctcc
    37741 agctgttatt tgacctacta aaggaagctc tattatttct tgattagctc cagaaacatc
    37801 atttacttta ctacgttcta aaacttcaat agctcttggt ttagtagggt cctttcttat
    37861 gtatcccagt ttttcaagtt tatttagatg agagtgcaca gttgaagtag atcttaaccc
    37921 tacagcagta catatttctc ttacagcagg tggataacct tttaatatga tttgagactt
    37981 tataaattcc aatatcaaga cttgcttttc agttagatct aaatacataa catcactcat
    38041 ttccaaacat attttgtttt tatttccata cactttatat ttttaattat aacataatcc
    38101 atttatatct gcaaacattt gttcctgatt tttaatcgtc ctcaatccat tcaaaattct
    38161 tgactatatc acgttttaca tttttcttat gagctgcata aagctgagta gttgtgacat
    38221 tatcatggtc aagtaagttt tgaacatcat atatagaaaa tcctgtttgg attagagaag
    38281 ttgcagccgt tgctcttaat ttatggggac tatatccatt gtctctagta gtatcaagtg
    38341 gaatagatgt atattttttt accagtgttc ttatagcctt tgctgtaatt ctcttatttt
    38401 gaagagacaa aaataaagca tctttttctt catcatttaa tagttcatct cttgttcttt
    38461 cgttttgcaa ataatctttt attacatgtt cacaagtatg atttataggc attaaaactt
    38521 ctttcccacg ttttctatat attttaaatt cacctcttga aaaattaaaa gaggagatat
    38581 tgagttctct aagctcattt aatctaagcc cgtatgtaac aaataatgct agtatagctt
    38641 tgtcacgtaa tttagttttt ctccaatata ccttttcttt ctctgtaagc ccttcacctg
    38701 tttctactga ttcaagcatc ttagcaactt catctatctc aaggcgctta atagcatctg
    38761 gctgaggttt aggaagtttt attggattaa atccatcagt tatattatta tctatctgag
    38821 agtttctgta taagaattta aatagagtag agatggatga tttttttctt gctaaagctc
    38881 tattgttatt ttcaaaaatc aatgtgtttt tttctgtatt tttgtaatat ctactgcaat
    38941 aatcacctaa gaataaattt atatctcttg attttattgt attaaaatca tcttctgtaa
    39001 tatcttttac acaatctgca tttgttaatt ctttggtttc aatcaaatat gaacaaaaaa
    39061 aattaatatc ttctaaataa gcaagtcgtg tagaaactgc aacagaacct tttaaatata
    39121 taaaataatc cttcataaat acaggaagtt tattttccaa aagtagacat ttttctatga
    39181 gttcattttc tttgataaca atattatcag gtttatctga tttattatgt acttttataa
    39241 ttttcttatc attattcaaa attaaacctc ctaaaactaa aatataaatc ttttatatta
    39301 ttttttgata tttataccaa gtgagtataa atatttaatt taatcatttt ttacattttt
    39361 aagagattag cagtgcttta gatgaaatta tcactaacta ttccctaaat ccacatttaa
    39421 agactagttg aatttaagta ctctttttta tttctcaata tatttataga aatattttaa
    39481 aatatattga gaaatttttt atgctctact aaaatctttt ttatataata tttttatatg
    39541 tttctaacga aaccaaatat tagtaaaata tgtatttatt taactaaagt ttttaaatgg
    39601 gctgactaag cttaaaatat aggatattat gagagttact attgaaaaaa taaaccctat
    39661 tgccataaag aaacttataa atatgttata cttaactatt aaactggata tattcaagtt
    39721 atcaaatcca aataagtatg taattgaact tattacaaaa ataactataa ctataaagct
    39781 cagaccattt aagctatttt ttaaatcagc actactaagt gacatatgtg tagatataga
    39841 atacattaaa aacataaata taaaatactt caatgaatat acactaggtg ttaacaacat
    39901 tattcttata aaattggaaa tatcacctaa aataatgtgt ataaaaccat ttatatttaa
    39961 attattaatc atacttacat ataactttaa atctatattt tgagtcactt tcatataact
    40021 atctggcagt aatatcttaa aaattaaaat aatcattatt gttccaccta taataggtgc
    40081 aattcctata aagaaatttc ctatactttg ataaagatta tcttttttat agctatgact
    40141 tacatatcct agtataccat catttttact ttctattggt ctgaaaaatt taactgaatt
    40201 tattttatgc atgaaaatta aacacattat caaatgactc aattcatgaa caaacgttcc
    40261 tatagctcca gttacaataa ttccagtttt tccaaaagat gaaaatataa atttattatt
    40321 tagattttct gtaatgctaa aaatcaatcc aaatacaata aatattccta tgaaacaaaa
    40381 aatatttatt acacttattt ttaaaatttc gattatataa atcataagtt aagtcctcat
    40441 gtaaaagtat ttaactactc tacttcatat ccattacagc ttaaaatttt acaagcattt
    40501 tctatatctt tttcttttac taagatatag tctgtatcat aagtagaaat tgcaaatatg
    40561 cttattttgt tgtctgctaa caaactactt aacttagata aaattcctac aagtgaaaag
    40621 tctaaaggac ctagtatctt aagtattctc cactcctttt ctgacttaac atcagaagga
    40681 atattattat ttaaacatac aacacttaat tcatcatcag ttctagttat agagtaaaac
    40741 ttctctgtat ctatccatgt tggtatttta ctgtcattat ttaatctaca tactgcatac
    40801 tcttctacca ataattttaa tttcataatg tatatttcct ccaatcttat ttgtttactt
    40861 aagattatac attaaaagtt acataaaata aaacatataa agtaaaaaag gttcactata
    40921 gtgtcctatt tctatagcca acctttaaaa cttaattaaa ttctaataaa gagatatatt
    40981 atctaggttc aattataagt ttaatggctg ttttcttatc accttcgatt tctatatcag
    41041 taaatgcggg aatgcaaact aaatccattc cactaggtgc aacaaaacct cttgctattg
    41101 caattgattt tatcgcttga tttagagctc cagctcctat agcttgtatt tctgcaacgc
    41161 ctcgttctct tagtactcca gccaaggctc cagctacaga attaggatta gattttgatg
    41221 aaacttttag tacctccatt tcaacatctc cttgttaatt tgttaaagta tccattacct
    41281 aaagtactaa atcataattg ccatttacta attacaatca tcatttaaag tatactttta
    41341 taaaaatata cttctataat cgttttaaga aaacctttat tttttttaac cattttatta
    41401 tgaaaaaact acaaatttct ttatattcta taagcatttt ccaatatttc taaatttttt
    41461 atatctttga gctataattt cctctttatc catatctttg taatgattta atctttctaa
    41521 aatataagct tttatttctt ttgaaacagc ttctacatct ttttgtgctc ctccaagagg
    41581 ttcttttata atcttatcta ttatttttaa atcatataaa tcattacttg taagcttcat
    41641 aacatcacaa gcttctttag ctttagatga atctttaaat agaatactag aaagcccttc
    41701 tggagaaatt acagaataaa ctgaatgttc taacatacaa acatcatttc ctactccgat
    41761 acctaaagct cctccactac caccttcacc aattacaaaa gtgattactg gaacagaaag
    41821 tttactcatt tcaagtaagt ttcttgcaat agcttctcct tgtcctcttt cttctgcttc
    41881 tagaccacaa aaagctcctg atgtatcaat aaacgttatt ataggtctat taaatttttc
    41941 agcttgtttc ataagtctta atgcttttct gtatccttct ggatgaggca ttccaaaatt
    42001 tctttttata ttttcttttg tatctcttcc tttttggtgt ccaactatag taacattaaa
    42061 ctttcctatc tgaccaattc ctccaaccat agaagggtca tctttaaaaa gcctatctcc
    42121 atgaagttct aaaaaattag aacaaatatg ttctatgtat tccaatgtcg taggtctctt
    42181 tatatctcta cttagtgtta ccttttcata tgctgataga tgtgaaaaag catcttcctt
    42241 aagcttttcc aatttctcat taagtgaatt tattttatca cttaaatcta tattatttgc
    42301 cttagaaata gttattaatt gtttaatatc attttctaag acttttattt tttcactatt
    42361 attttcaatc aaaacagttc ctcctttatg ccctatttct tgtgcatagc aagaatttga
    42421 tataatactt ccttcatttt tcttctatct actatcatgt caataaaacc tttttcaagt
    42481 aaaaactcag aagtttgaaa tccttcagga agtttttgat ttattgtttg ctctattact
    42541 cttggacctg caaaacctat caacgtattt ggctcagaaa ttataatatc accaagcata
    42601 gcaaaactag cagttactcc tccagtagta gggtcagtta gaactgatat atatggcaat
    42661 gatttttctt caagcttaga aagtgcttga gaagtctttg ccatctgcat aagagatacc
    42721 ataccttctt gcattctagc tcctcctgaa gcagaacaaa taattatagg taaattattt
    42781 tcagcagcat actctattga ataagttatt ttttccccaa caatagaacc catacttccc
    42841 atcataaaat caggattcat tacacaaata actgttttaa ttccatttat tcttccataa
    42901 cctgtgacaa cagcttctga ctcttttgtt ttttccttat aagagtctac tttttctttg
    42961 tatttaggaa aatttagtgg attttgaaat tctacatcag aattaaactc cataaaagtt
    43021 cctttatcaa tcaataaatt tattcttttt cttgctctta agccataatg tcttgaacat
    43081 tttggacata caaatgaatt ttcttctatg tcctttctaa atacatgact atcacaacct
    43141 ttgcaatagg tccaaaattt atcatctaca ctattctttt taaagttgtc atctaaagaa
    43201 atcgtaacat acttagattc ttttgatgat aaaaattttt ttatcataat ctatttactc
    43261 cttataatgt agcaaattct ttcttcataa agacatcttc tataaatttg gtatgatgag
    43321 tatcttctct aaactcctca cttgttacaa gtgccttttg aaattctaca tttgtattaa
    43381 taccttctac aataatttcg tctagagctc tatatattct acttatacat tcttctctag
    43441 tttcagccca acatattaac ttacctatca tagaatcata taaaggtggt atagtatatc
    43501 ctgcatatac aaaagtatca aatctaacac caaaacctcc aggtaaattt aatgattcta
    43561 tcttacctgg tgatggagca aaattctttg atgaatcttc tgcatttatt ctacattcaa
    43621 tagcatgacc tctaaaagta atatcttctt gtttaaaagg aattttatcc ccattagcta
    43681 tagatatttg aagttttata aggtcaagat ttgttaccat ctctgtaact ggatgttcaa
    43741 cttgtattct tgtattcatc tctataaaat aaaagttttt atctttgtca actataaact
    43801 caatagtacc tgcaccgata taacctactc cttttactat atctactgca attttatgaa
    43861 gtttttgtct ttcttcatct gaaaggtatg gacttaaaga ttcttctatt actttttgat
    43921 ttcgcctttg catagaacaa tctctatcac ctaaatgtat tgcatttgaa tgtttatctc
    43981 caaatacttg tacttctata tgtctaggat tttctataaa cttttccata tataaatcac
    44041 tattagaaaa tgctgctaga gcttctgaac ttgccatatc aaaatttgaa aaaagttctt
    44101 cttcttttct aacgattctc ataccttttc caccgcctcc acttgaggct tttatcataa
    44161 caggatatcc aatttctctt gcaacttcta aagcttcttc cttagttttc aaaacagatt
    44221 ctgaaccagg aacaacaggg atattcaatt ccatcatagt ttctcttgct ctagatttat
    44281 ctcccatcaa aatcatttgt tctcttgttg gacctataaa aataatatta ttatcttcac
    44341 attgctttgc aaattcagga ttctctgata aaaatccaaa gcctggatga atagcatcac
    44401 actttaattt aagagcaaga tagattatat tttctatatt attataactc ttacttatat
    44461 tatttggtcc tatacaaata gattcatcag ctatatatct atgaaaacaa tctttatcta
    44521 tttcagaata gatagcaaca gtttttatac ctagttcttt acatgttctg atgattctaa
    44581 ctgctatatc gcctctattt gcaactagta ttttttttat caacttaaat acctcctact
    44641 acaatggttt tatcttaaat aaaggttgat tatattctac tagttcttca ttactaacta
    44701 atacttcaat tatttcacct ttaatttcac ttgttatctc attcataagc ttcattgctt
    44761 ctaatataca taaagtgtcg ccttcttcaa ctacatcgcc aactttaaca aagctatctg
    44821 catctggact tggagaatca tagaatgttc ccatcaaagg agctactact actgatagat
    44881 tctcattaga tgtgacaggt gtatcgttta caactggctt ttctaaagaa agatccacta
    44941 ctgattcttg ttgtacactt aaaacaggtg aacttgtaga ttgagctttt ttagatactt
    45001 ctaatcttaa atcgctactt tcaagtttta catattctaa gttagttgag tctatggcct
    45061 ttaataattc cttaatttca ttgatgttca ttttgttacc cccttgtatt attacctggt
    45121 gctaatatta tatcacaaaa aacaaattta taaaagaaaa agtgctgact agagaatcaa
    45181 cacatcaata tatcttttaa ttttaaatta acaaccagta acttatattt gttttaattt
    45241 tccattatta atataaatta taagtataaa atgaataaaa ttcaatattt tttgcaaaat
    45301 acaagagaat acaaattaat acctcgggaa tttttaagac aaataccaat attatgcaat
    45361 ttagacttcg ctttctttca aattagtatc attttcttta tttttacata ggatgtactt
    45421 taatttagct atcaaaacat tgctatatag gttaaaagtt attagaattg caggaattat
    45481 aaagtaaact ctagcaccta taaaaccaaa aactactgta ataagtatta atcttttatt
    45541 taaagtaaaa aaaggtatat taaaaatact tttgtcggat gtttctggag tataagaata
    45601 tataactcca aaaatcaaag taagcataca aagtggaagt agtgtataac ttgaaattat
    45661 tcccaaaaat cctatacttt caaaatattt gctagcacta tttgtataaa caaacatgta
    45721 tccatttaaa tatgcgcata tacatgatat aatgccaaaa gttcccccta aggattgagt
    45781 gtaccaatta aaaaatttag attccatata cctcacctcc taaaactgta tatataatga
    45841 tatgcattta aatagtaaaa tatatcttta tatgtttctg aataaaattt tgttaatttc
    45901 attaatttat gtttaagaaa tttgaagtat agtgtagtat atattaatgt agatgaagtt
    45961 ttatagtact tatttcatag tgcttaatca agttatctta ttatggattc aaaagttgtt
    46021 tgtagggttt tatattttgt tgagctgaaa aatttattat aaatgaaggg atgttttcgc
    46081 tttggtagat acattagaat ttggtcttaa aattttattt tttatattat caataatttg
    46141 gatgggtaaa ataatgattt taagaactga taagcaaata gtaatcaatc ctttgcttat
    46201 tggtatatct gcaatccttg tgatgttgca tacctcacag agtaacattg aattttttgg
    46261 attagatgtc caatatataa gaatagtttt gtatataata tattcactga taattttaat
    46321 aggaatatgg gctactaaca gaagaaatgg aattttttag ataatctatt tctataaaat
    46381 ttaaaggttg ctagtaacaa atcactaata ttagcgttct gttactaaca acctttattt
    46441 tgattataca cttaaattga aaatagaatg attgaataag ttgacagatt attttacatt
    46501 gacagtaatt agaacttctt gagatgtatt taaatcattt ttgtcgctta gtataacttt
    46561 taccactgct ttacctgttt tattaccttt aattatcata tcttcacatg aaagtacact
    46621 cttatcactt acagatacaa taaaatttaa atcttttgga aggttatcag taacttccca
    46681 gtatttataa taactatcaa ttttcttcgt aattattttt ctcaaatcaa tactttcacc
    46741 ttttgctata tcaacattat gatatttaga gtccagttta tactgattat cttttgtatc
    46801 aataaaattt aaatcgcaat ttacatccac accattatga gaaagagaat ttactacatc
    46861 gtcatcaatc aaataattat tggcaagatt tattgaatcc atatctttta gacttaaagc
    46921 ttccttagga acctctttta tattattatt ccaaaaaata aaattgtcta cattttcaag
    46981 atatgatata ccttttaaat ttgaaatact ctcaccagtt gcataaaatt ctccatctaa
    47041 agcctttata ttgcttatag tcatctcatc tggatttata tatcttttta caactgctct
    47101 taaatttgca tcaggaaaaa tatctttaag ttgattttcg ctcaaaacct tatcatttga
    47161 atttattgga cttaaagctg ctgcagaaat aataaatggt attaaattag ctaaattcat
    47221 agtaccctcc cacataaatt ttttattata ataatttaat ttctatgaga gggttctgtt
    47281 tccttgttat atatacctac tttcgtacac tttattaata taaattaaaa taaatttcta
    47341 ctattaaact tataaacata ttactagtta cgttttttag cttcacatct aaatattcgc
    47401 ataccttgtg acataaattt gtcttcatac tctgttgtaa cattattttc atactcacta
    47461 tttcctaaat caagagaaat attctttaat agccaattat ttgcagcaat ttcatttaaa
    47521 ctaaactcga ataatttttc attatctgtt ttaaagtgaa tttcgccatc atcttccaaa
    47581 accctattgt acatttctaa aaatccagaa tgagtgagtc ttcttttact ccatctattt
    47641 ttaggccatg ggtcacagaa atttatataa attctcgata attctttagc ttcaaagtaa
    47701 tccaaaatat tgcttacatc tccccataaa aatagtatat tattcaaatt agaagcatct
    47761 gctttttcaa cagcttttaa aagaacttct tccttaagtt ccatggcaat ataatttatg
    47821 tcagggttct gcttagctaa agttgttata aacttacctt ttccagtccc aaattcaaca
    47881 tgtattggat tatcatttct aaactttaaa ttccacttgc cttttaattt gtctatatcg
    47941 tctcttaaaa catattttgt ataacttaaa agcttttcat ctgcaccttt ttttcgtctt
    48001 cttctcacaa taattctcct tacatataaa aaattagtaa aatacatttt tattatacat
    48061 tatctcacca aaaaataaaa agaactctct taaaatagaa atttatttct ataaagagag
    48121 ttctaaactt actaaaaact taatcttagt ggcaccctga acaaccagag catcctcctc
    48181 caccttgatc tttcatattt tcaggaataa ctctaaatcc aaatcctgta tcttctatta
    48241 ttatatctcc atactgacta tattcatcag agctcattac aaattgtaat tctccaactt
    48301 cataagttaa gtcatcttct tttttctcat ctaaagctaa tccaaaagaa ggacctccac
    48361 aaccaacacc tgcaaagtat actcttatat tattaggttt atcttgattg tcttttaaga
    48421 tactttttaa agtatcaata gctgtttctg gtaaagtaat tttcatcaaa tcattccctt
    48481 tcaaatattt tattaaaaca atatctatta tatacccata caaggtacat aatcaattag
    48541 tttttgcata ttttactaaa acttctacct aatattacct aaggctttaa aatattttat
    48601 at
//
LOCUS       DAMJYC010000024        46985 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJYC010000024 DAMJYC010000000
VERSION     DAMJYC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.025"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(410..784)
                     /locus_tag="PC472_001434"
     CDS             complement(410..784)
                     /locus_tag="PC472_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726765.1"
                     /translation="MEKQNLEDNERRQRLKDIRLSKKLKQKDFGKKLGLSLSQISSYE
                     TGHRKIPQRTINDICREFNVNEQWLLHGEGEMFVDITEDLETSEEIKTMIKKIFALED
                     EDREAIEKIIDNAYLKIKNEEL"
     gene            938..1114
                     /locus_tag="PC472_001435"
     CDS             938..1114
                     /locus_tag="PC472_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726766.1"
                     /translation="MNQEKEKANDLLLKLELLKKINPQEFGYISGRIDVNIEKLKQET
                     ERNKSKKIRYRKGA"
     gene            1118..1324
                     /locus_tag="PC472_001436"
     CDS             1118..1324
                     /locus_tag="PC472_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726767.1"
                     /translation="MTFNKNQIGTAVQLQDIRFDTKVDCIITKVEKNEILVMYYEKET
                     EEIAYKTLTKEDLILDDYKLKLLY"
     gene            1353..2075
                     /locus_tag="PC472_001437"
     CDS             1353..2075
                     /locus_tag="PC472_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726768.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLSVYEKMAYMVMARYSDDES
                     SCFPSLSLISKKMGCGITTVKKTINSLEEKNLIIKVHRKVKNKKENDTNVYYIMSLKG
                     KSSGDIGHEATDGRSPYDQQVGREATSKKTYNKKTYIKSNTTPQNEPKTDYLDLSFLD
                     LDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWGNGD
                     ASKGIVPEVTKTKNPLSGFKEL"
     gene            2103..2288
                     /locus_tag="PC472_001438"
     CDS             2103..2288
                     /locus_tag="PC472_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726769.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2314..2532
                     /locus_tag="PC472_001439"
     CDS             2314..2532
                     /locus_tag="PC472_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726770.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2551..2763)
                     /locus_tag="PC472_001440"
     CDS             complement(2551..2763)
                     /locus_tag="PC472_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726771.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            2926..3186
                     /locus_tag="PC472_001441"
     CDS             2926..3186
                     /locus_tag="PC472_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726772.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3179..3367)
                     /locus_tag="PC472_001442"
     CDS             complement(3179..3367)
                     /locus_tag="PC472_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726773.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3505..3822
                     /locus_tag="PC472_001443"
     CDS             3505..3822
                     /locus_tag="PC472_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726774.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            3844..5175
                     /locus_tag="PC472_001444"
     CDS             3844..5175
                     /locus_tag="PC472_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2726775.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDVGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIVDINDLKALVTTSSSINGNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAEAIANKEKAFLYSG
                     ELPSFMAMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRLFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVIDEEEEADDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQAEIPEDVF"
     gene            5549..5935
                     /locus_tag="PC472_001445"
     CDS             5549..5935
                     /locus_tag="PC472_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726776.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSASVIDFRNASSK"
     gene            5987..6691
                     /locus_tag="PC472_001446"
     CDS             5987..6691
                     /locus_tag="PC472_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726777.1"
                     /translation="MIRNAIEYLVKLGKRETVCVDGFKYTTDALEIIEEPKANELEIT
                     TLSGLVDYIKSGIDHKEDELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6749..6931
                     /locus_tag="PC472_001447"
     CDS             6749..6931
                     /locus_tag="PC472_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726778.1"
                     /translation="MKSISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            6928..7305
                     /gene="ssb"
                     /locus_tag="PC472_001448"
     CDS             6928..7305
                     /gene="ssb"
                     /locus_tag="PC472_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2726779.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLTKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEKEIGFQVLDDEDIPF"
     gene            7283..7867
                     /locus_tag="PC472_001449"
     CDS             7283..7867
                     /locus_tag="PC472_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726780.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            7896..8054
                     /locus_tag="PC472_001450"
     CDS             7896..8054
                     /locus_tag="PC472_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726781.1"
                     /translation="MKTKIIGGRENECPICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            8057..8383
                     /locus_tag="PC472_001451"
     CDS             8057..8383
                     /locus_tag="PC472_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726782.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8383..8919
                     /locus_tag="PC472_001452"
     CDS             8383..8919
                     /locus_tag="PC472_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDF2726783.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            8931..9737
                     /locus_tag="PC472_001453"
     CDS             8931..9737
                     /locus_tag="PC472_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726784.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9762..10022
                     /locus_tag="PC472_001454"
     CDS             9762..10022
                     /locus_tag="PC472_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726785.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNNIDNENVLNGEM
                     DLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQRELGLQ"
     gene            10023..10664
                     /locus_tag="PC472_001455"
     CDS             10023..10664
                     /locus_tag="PC472_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2726786.1"
                     /translation="MNKRIVCQDTNEIVYNYKEYQKTKHWESLKNEFYFSNECKCVLC
                     DSVKKVSLHHKTYRRIGAEELDDLIPLCNSCHYKVHRNNKQYLFNKATEKQIRCINDL
                     LEVYVDIVEELFMNEYIYLYVAMGIEFYNENLMLFEANHIINDLKFVSAMLYEFYATK
                     LEIKLPGYFNGFDYEEIKDSLYKKMQRLSKYIYVPKKQEIELLFKKRKKEHYE"
     gene            10719..11204
                     /locus_tag="PC472_001456"
     CDS             10719..11204
                     /locus_tag="PC472_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726787.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            11224..11481
                     /locus_tag="PC472_001457"
     CDS             11224..11481
                     /locus_tag="PC472_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726788.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            11590..12033
                     /locus_tag="PC472_001458"
     CDS             11590..12033
                     /locus_tag="PC472_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HDF2726789.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            12176..12721
                     /locus_tag="PC472_001459"
     CDS             12176..12721
                     /locus_tag="PC472_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726790.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDIIEEISSAAYVPDNLKYYDNVM
                     RETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILDESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            12725..13369
                     /locus_tag="PC472_001460"
     CDS             12725..13369
                     /locus_tag="PC472_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2726791.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDIKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            13584..14042
                     /locus_tag="PC472_001461"
     CDS             13584..14042
                     /locus_tag="PC472_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726792.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGTTVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            14597..16336
                     /locus_tag="PC472_001462"
     CDS             14597..16336
                     /locus_tag="PC472_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726793.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            16337..16540
                     /locus_tag="PC472_001463"
     CDS             16337..16540
                     /locus_tag="PC472_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726794.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            16553..17830
                     /locus_tag="PC472_001464"
     CDS             16553..17830
                     /locus_tag="PC472_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2726795.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17808..18569
                     /locus_tag="PC472_001465"
     CDS             17808..18569
                     /locus_tag="PC472_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2726796.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18628..18954
                     /locus_tag="PC472_001466"
     CDS             18628..18954
                     /locus_tag="PC472_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726797.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18980..19582
                     /locus_tag="PC472_001467"
     CDS             18980..19582
                     /locus_tag="PC472_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDF2726798.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19611..20534
                     /locus_tag="PC472_001468"
     CDS             19611..20534
                     /locus_tag="PC472_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726799.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            20548..20820
                     /locus_tag="PC472_001469"
     CDS             20548..20820
                     /locus_tag="PC472_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726800.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20804..21184
                     /locus_tag="PC472_001470"
     CDS             20804..21184
                     /locus_tag="PC472_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDF2726801.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            21196..21543
                     /locus_tag="PC472_001471"
     CDS             21196..21543
                     /locus_tag="PC472_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726802.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            21543..21899
                     /locus_tag="PC472_001472"
     CDS             21543..21899
                     /locus_tag="PC472_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2726803.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKNGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21908..22357
                     /locus_tag="PC472_001473"
     CDS             21908..22357
                     /locus_tag="PC472_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726804.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            22358..23413
                     /locus_tag="PC472_001474"
     CDS             22358..23413
                     /locus_tag="PC472_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2726805.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMLD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            23429..23869
                     /locus_tag="PC472_001475"
     CDS             23429..23869
                     /locus_tag="PC472_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2726806.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23884..24300
                     /locus_tag="PC472_001476"
     CDS             23884..24300
                     /locus_tag="PC472_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2726807.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            24327..24476
                     /locus_tag="PC472_001477"
     CDS             24327..24476
                     /locus_tag="PC472_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726808.1"
                     /translation="MYLLFKYKGIMPSVSYRILRGEKEIVSVFMDQELDEREEEIKSG
                     AGRLF"
     gene            24480..27869
                     /locus_tag="PC472_001478"
     CDS             24480..27869
                     /locus_tag="PC472_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2726809.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRTLN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKDMTDKQKNYYLAAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPIGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFNLIIGIVTGDSKKMMNG
                     VKQIFDGGVKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWKDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTIANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSFFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            27916..28392
                     /locus_tag="PC472_001479"
     CDS             27916..28392
                     /locus_tag="PC472_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2726810.1"
                     /translation="MNKKIPALAILIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTDLESDFLQNYILKTDDNTV
                     GNAINNQELNGELLFDTIHPGDTLKGEIAFELTKDTKPKTIEIAYENVGKYTVFDL"
     gene            28450..29085
                     /locus_tag="PC472_001480"
     CDS             28450..29085
                     /locus_tag="PC472_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2726811.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKDVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDKIKDPSVIKNGWILIIP"
     gene            29325..30842
                     /locus_tag="PC472_001481"
     CDS             29325..30842
                     /locus_tag="PC472_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2726812.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGTYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNVTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30856..31188
                     /locus_tag="PC472_001482"
     CDS             30856..31188
                     /locus_tag="PC472_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2726813.1"
                     /translation="MADPINEFIGIMRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGDTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            31181..31615
                     /locus_tag="PC472_001483"
     CDS             31181..31615
                     /locus_tag="PC472_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2726814.1"
                     /translation="MNNTIFPFMGVPEDYISPTNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     ETLLTNPYILEVNVTNTKFKDDNLSISLNVKTIYGEEEITFA"
     gene            31608..32660
                     /locus_tag="PC472_001484"
     CDS             31608..32660
                     /locus_tag="PC472_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2726815.1"
                     /translation="MHNGQSFSVLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTII
                     SYSDLLFVVIKDITIDEDSKLNVSPIQALEVGIKYNLSAKTEFKLIEEINGVTKIYNE
                     LDIKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTITIVTPSTFDITISATLTLENGY
                     DIESIKVMFLDIINSYLIENSKEIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32662..33279
                     /locus_tag="PC472_001485"
     CDS             32662..33279
                     /locus_tag="PC472_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2726816.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLDQFFID
                     TATWGLDDWESLLDIKINYKLDFDTRRSNIKAKLRGKGTTTIEVIKAISEAYTKTNVD
                     VKVFSNLFSFTLSFLTNDCSYNTILELDKKIEEIKPCHLEHKFEMILFNKNNMFTGST
                     MNTGETVTIYPYSTTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            33281..34105
                     /locus_tag="PC472_001486"
     CDS             33281..34105
                     /locus_tag="PC472_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2726817.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGAEYN
                     PTEEQTSLKNVVWEGSINNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQDLDSKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVNALNEVKTSVDSIETTAEKTS
                     IKDIDNLFESDNVEGALNQLAINYNILLEKQDNLETEVNGQRIKGISIANSLIDMI"
     gene            34119..34715
                     /locus_tag="PC472_001487"
     CDS             34119..34715
                     /locus_tag="PC472_001487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726818.1"
                     /translation="MTEKLTDNASLRELMIAFESAKNDLQIDKNNIASTLGSPFLGTD
                     KLDTTKTKTQTLKNTLASNITNKGIVTNSTESLQDMISKVSSIKDLRQASGTASAFKG
                     RFYDDYYYLWSVNLPQLNFVPLVVFVYADYRRLSIWVKSPFSKYIFYGKKDNVKTELK
                     DFYIKELDDGISTETVIHLYGASGDTYEVQWLAIGQEA"
     gene            34718..35011
                     /locus_tag="PC472_001488"
     CDS             34718..35011
                     /locus_tag="PC472_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726819.1"
                     /translation="MEVGKLIIYDNKGKILFQGGEGKGDLIPYDKDMKVDWVEIKYNS
                     IDYSKYRIIGINVETKEPVLEEIKVELTEEEKRIQELENQILIAENEKVGGIL"
     gene            35011..35193
                     /locus_tag="PC472_001489"
     CDS             35011..35193
                     /locus_tag="PC472_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726820.1"
                     /translation="MNINNVVVRILAERILSGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIRESGVIEGTELTV"
     gene            35233..35463
                     /locus_tag="PC472_001490"
     CDS             35233..35463
                     /locus_tag="PC472_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2726821.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            35479..35736
                     /locus_tag="PC472_001491"
     CDS             35479..35736
                     /locus_tag="PC472_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2726822.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLAL
                     GIGFSIWMLGLNPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            35736..36539
                     /locus_tag="PC472_001492"
     CDS             35736..36539
                     /locus_tag="PC472_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2726823.1"
                     /translation="MKIAIVPGHTLSGKGTGTTGYIDEGKENRILTDLIVKWLKQGGT
                     TVYTGKVDKSNNYLAEQCQIANKQDIDLAVQIHFNANKTTLNAMGTETIYKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDARGLYWLKHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIK"
     gene            36598..37367
                     /locus_tag="PC472_001493"
                     /pseudo
     CDS             36598..37367
                     /locus_tag="PC472_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(37796..38311)
                     /locus_tag="PC472_001494"
     CDS             complement(37796..38311)
                     /locus_tag="PC472_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726824.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRVGNRFLEDNKSYDDKYHIDSINSKIDDDIKYLSNEIDNIKSVIEWFKTK
                     DDKCHIDSIKENDISIDLPDQSIKRTTIRVNNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(38394..39299)
                     /locus_tag="PC472_001495"
     CDS             complement(38394..39299)
                     /locus_tag="PC472_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDF2726825.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFAKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSVNVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYIDK
                     GFYLNGEKQDIKKNIDASVGLFKQIYNELKLNYPISTSAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(39318..39521)
                     /locus_tag="PC472_001496"
     CDS             complement(39318..39521)
                     /locus_tag="PC472_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726826.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(39771..40229)
                     /locus_tag="PC472_001497"
     CDS             complement(39771..40229)
                     /locus_tag="PC472_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726827.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLHWRVTSTLGNILGFEGNIDVYKAGKIVSSQFVS
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(40264..40566)
                     /locus_tag="PC472_001498"
     CDS             complement(40264..40566)
                     /locus_tag="PC472_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726828.1"
                     /translation="MEVNLHTGKDEFFKNHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(40954..41106)
                     /locus_tag="PC472_001499"
     CDS             complement(40954..41106)
                     /locus_tag="PC472_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726829.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(41226..41960)
                     /locus_tag="PC472_001500"
     CDS             complement(41226..41960)
                     /locus_tag="PC472_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2726830.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(41980..42780)
                     /locus_tag="PC472_001501"
     CDS             complement(41980..42780)
                     /locus_tag="PC472_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2726831.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            complement(42800..43987)
                     /locus_tag="PC472_001502"
     CDS             complement(42800..43987)
                     /locus_tag="PC472_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDF2726832.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44407..45096
                     /locus_tag="PC472_001503"
     CDS             44407..45096
                     /locus_tag="PC472_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2726833.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45245..45628)
                     /locus_tag="PC472_001504"
     CDS             complement(45245..45628)
                     /locus_tag="PC472_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2726834.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            complement(46393..46761)
                     /locus_tag="PC472_001505"
     CDS             complement(46393..46761)
                     /locus_tag="PC472_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726835.1"
                     /translation="MAEVSKKLKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
BASE COUNT        18850 a         5496 c         7664 g        14975 t
ORIGIN      
        1 aaaaaaaaat gaaccaactg aatggagaac tatagaaacc ataatctcca gtacatacgc
       61 attaagtaat ttcaaaaaag aaaataaaga cattatgaat cagaaataac aacagtaaat
      121 acttaagggg agtgataagc ttgaaaattt cagattttaa taaagatcat ataggtaaag
      181 cgattcaact acaagagaca agatttaatg tcataacgga ttgcatcatc acagaagtag
      241 aagtaggcga aattgttgta atgttttaca tgcaagaaaa tgatgacaca ggatacaagg
      301 ctataacatg ggaagattta aaaaattata tcataaatgg caaagggaag aggcattata
      361 ggatttttgt ggcttggggg attgttataa aaagaggtat aacctctttt tataactctt
      421 catttttgat ttttagatat gcattgtcaa ttattttttc tatcgcttct ctatcttcat
      481 cttccaatgc aaatatcttt tttatcatag tttttatttc ttcactagtt tctaaatctt
      541 ctgtaatgtc aacgaacatt tcaccttctc catgcaataa ccattgttca tttacattaa
      601 attctctaca aatatcattt atggttcttt gtggtatctt tctatgccct gtttcatatg
      661 aagagatttg cgaaagagac aaacctaatt ttttgccaaa atctttttgt tttaatttct
      721 ttgaaagcct tatatcttta agtcgttgtc ttctttcatt atcttctaag ttttgttttt
      781 ccataacgtc accccttatg agtataatta taacatttaa aaatacgctt tgcaattaca
      841 aattttaaaa aaatacgcaa aataaaataa aaactcaaag cgtattgaca tagtatgcaa
      901 agcgtaatat aattgaatca caggaaggag tgttagaatg aatcaagaaa aagaaaaagc
      961 gaatgatttg ttattaaaac ttgaacttct taaaaagata aacccacaag agtttggata
     1021 cataagcgga agaatagacg taaatattga gaagttaaaa caagaaaccg aaagaaataa
     1081 aagcaaaaaa ataagatata ggaagggggc ttaaaaattg acttttaata aaaatcaaat
     1141 aggaacagca gttcaattac aagatataag gtttgataca aaagttgatt gcatcattac
     1201 aaaagtagaa aagaatgaaa tactagtcat gtattatgaa aaagaaacag aggaaatagc
     1261 atataaaact ttgactaaag aggatctaat acttgatgat tacaaactta agttattata
     1321 ttaaaaagta atttcggaag ggaggtgatt aaatggatct tttgaaagat gcaagaaaaa
     1381 aagaatggtt ttggctggaa aatgatttgg tggatagaga ggatctaagt gtttacgaaa
     1441 aaatggctta tatggtaatg gcaagatatt cagatgatga aagttcttgt ttcccaagtt
     1501 tatcattaat atccaagaaa atgggatgcg gaataacgac tgtcaaaaaa acaataaata
     1561 gtttagaaga aaaaaattta ataataaaag tacatcgcaa ggttaaaaac aaaaaggaaa
     1621 atgatacaaa tgtttattac attatgagtt tgaaaggtaa gtcgtctggc gacataggtc
     1681 atgaggcgac agacggtagg tcgccatacg accaacaggt aggtcgcgag gcgactagta
     1741 aaaagactta taataaaaag acttatataa aaagtaatac cactccacaa aatgaaccta
     1801 aaactgatta tttagaccta tcatttttag atttagatat agaaaaagta aaactaacta
     1861 aagatgaata tgacaaactt ataagcaagt ttgggaagaa atacatacat gataaaattg
     1921 ttagtttaga aaactatatc ataaatggta aagggagtag gtataagagc cactataggg
     1981 ctttgctgac ttgggggaat ggagatgcta gtaaaggtat agtaccagaa gttacaaaga
     2041 ctaagaatcc tcttagtgga tttaaagaac tttaaaaact aaatagtaag gaggtagata
     2101 ggatgaatga aaaagaagag actgtttatt tgaatagttc taatgtgaag ttattcttag
     2161 gcgttaggga tattaaggaa tttgaggaat tgatagaaaa tgtcaatgag cgaatttgtc
     2221 aactccaaga atcgattaat aaacttgccc aatttaatat tgagttcgat attaagtcag
     2281 atatttagtt aagtataaat taggaggttg cctatgaaag atgaaaagtt agaacttact
     2341 gaaaaagatt tacactgtat tgcaagacat ttacaaaatg aagttatgga gatggtgttt
     2401 agagggaata tagaagcccc tacatcatgc gaagtttgcg attacttgca agaatgcaag
     2461 gactatttca cccatataga cacttttata aaattaagtg aaatgacagg tgtagatatt
     2521 tttactaaat aaatttagta aattaaaatt ttagtattgt aattgacgtg cttttttaaa
     2581 aagaggacag ttgctattag cagtcattat ttgaattttg caaatgtaat ttttattaca
     2641 ttcgcattct aaaagttttg atccagtgaa tccaggaaaa gttttatatt caccagaaaa
     2701 ttcaacttta tcgtcaataa gagagcattt ttctcttaag gttttaattt tgcacatatt
     2761 catcaccacc ctataattga tttgaacata ctgtcaatat gctccaatta attataacat
     2821 gcaaattatc atggtgtaga attaaagcat aggtcaatta aatttagtta taaaatcaat
     2881 gaaacagata aagaattaaa tgttaaataa attagggggt tgttcatgaa agaggaaaag
     2941 ttagaactta ctgaaaaaga attgcattgt attgcaagac atttgcaaaa tgaagttatg
     3001 gagatggtgt ttagagggaa tagagaagcc cccacatcat gtgaagtttg cgattacctg
     3061 caagaatgtg agggagattt tacctgtatg tatcattctt ttactaaatt aagcaaaata
     3121 acaggtgtaa aagtgactgc atttaagtat tgttttccag atgaaaatcc caaagctact
     3181 atctaagaag tttttttaga ataggacatt cttcattaga agaagagtcg caaatatgac
     3241 ttccatcaca tctagttgac attaacttag atccaaaagt acctggagga gctgaatatt
     3301 cgcaagtaag agaaatggtt tcgtttatta caggacattc tttgcttata actcgatatt
     3361 ggctcatact ctcaccacct ttctaattaa tttaaacata ttggaagtat gtttaaatta
     3421 attatagcat gtaaattatc atggtgtaga atagtttcaa atcaaaagaa gcaactaaat
     3481 agttaaagac aggaggtatg ctaagtggaa agaaaaaaac atgaacttac agatcaagat
     3541 atacaaagac ttattgaagg attaagaact gatccaaaaa taagagaaat gatatttgaa
     3601 agtaaaggtt gtgaaactag aacaagtaca agaagtaaat gcgaacaatt tgttgaagaa
     3661 aaacttgctt atagaagtta tggatttgaa actccaaaat actatgcaaa acttattgcc
     3721 gacataattg atattttagt ttacaaaaat gttccaagag atcgtatttg tggggtattg
     3781 tatgacgtcg aaagggtgat tcctttagtt gtgacatttt aataataagc tgggggtgat
     3841 taaatgaatt actctttaga agcagagcaa aacattctag gaagttttct tataaatgac
     3901 tctataggtt acaaaataag ggaattaaaa gaaaatgatt tctactttga gtcacataaa
     3961 attattttat cttgcatgaa gaaaataatt gactcaaata aaccactaga tcttttgctt
     4021 ttaaaaaatg aattagaaaa aatagataga ttagtagacg ttggaggagt tagttacatt
     4081 accagtttaa caactatagt cataactact tcaaacatag atcactatat aaaaatcata
     4141 aaagagaagt tacttaaaag acaaatttca gagctagcaa atgaattaat taataattca
     4201 aattcagata caagtataga tgaattgatt gtagatataa acgatttaaa agcacttgtg
     4261 acgacgagca gtagtataaa tggtaattat attgacgcat caaaaataaa acgtgaaaaa
     4321 ggcgtacaca agtccataga cacgggtttt aataaactaa acaatatgct agatggattt
     4381 agatatggaa ctcttacaat tttgactggt aaacctgcat ctggtaaatc aactatagtt
     4441 aaccagttta tagcagaagc aattgcaaat aaagaaaaag catttttgta ttctggagaa
     4501 ttaccctctt tcatggctat ggattggttt agaaaaactg tagctaatga ttatcacata
     4561 aaagaatata aaagtgtcta tggaggaaca tacacagata ttccagatta cgccgttgag
     4621 cttatatcag actggataga agataaattt tttctctatg atgaagatgc aataagtgat
     4681 gaagtaaatc tactaaatac catagaacat ttatatttaa aaaaaggggt taggctcttt
     4741 gttcttgaca atctaatgac aataaaaact ggcaataagg cagataaata tgagagacaa
     4801 gagcagatag taagcaactt aaaaaatctc gctaaaaaat ataacttagt aatagtttta
     4861 gttgcacatc ctagaaaaaa tatgggtgac atgaaaccaa ctatgtatga tgtgtctgga
     4921 gcaagtgaga ttgttaatta tgcagattat attttatcaa cttacagagt tattgatgaa
     4981 gaagaagagg cagacgacac ttatttatta attttaaaaa atagaataac aggaaaacag
     5041 aatataagtt tcaaaatgaa tttttcagaa agaagaaaaa gactttacac aactagtgaa
     5101 gagttaaata gagattacaa atatgatgta aataaacaat atgtgcaagc agaaattcca
     5161 gaagatgtat tttaaaaaag gataaaaaat aggaaatttt attaagtaat gattttacaa
     5221 taaaaatcaa tattgaataa tttaaaaaat caatattgaa aaaataccgt ttttcaatat
     5281 tgaaattaaa atttcataag gtattgaaaa tcaatattga aaaaaattaa taaatatagt
     5341 aattgcaatg attgcagaat acgattacat tacacaaaaa acacaggttt aaagaatttg
     5401 tcaaaaacta ttaaaagatg gtaatgatag agagaaaaaa ataaaatcaa aaattttgat
     5461 aaaaatgtta ttaaattata gtcaaagttt attaattaat ttcctcgtag aaaaatatta
     5521 ttttaaataa ataaaggagt gattttacat gatagcatta gaaaaatttg caggtggagt
     5581 tctaaaagaa aagtttaata cagagttaca aaaagtttta gataatattg cagatccaaa
     5641 tacagatttt aaaaaaacaa gaaaaattag tttagagata gtgtttaaag caaatgaaga
     5701 tagagatctt gctgaagtag atataaaatc taaagcaact atagtagaag caaaggctac
     5761 aacaactaaa gttctgatag gaaaagattt agagacagga agagttgaag cgtctgaatt
     5821 taaaaatcaa gtagcagggc agttggtcat ggatattcca gatagtgaag aaactaatga
     5881 aacagaagaa aatagtgcta gtgtaattga tttcagaaat gcaagttcaa agtagtttaa
     5941 gaaagtaagt aaaatataaa tttaaataaa aaaggagaat aaaaaaatga taagaaatgc
     6001 aatagaatat ttagttaagt taggaaaaag agaaacagta tgtgtagatg ggtttaagta
     6061 cacaacagat gcattagaaa taatagaaga accaaaagct aatgaattag aaataactac
     6121 attgagtggt ttagtagatt atataaaaag tggcatagat cataaagaag atgaattgtt
     6181 attaatacaa gttctaactc caagaaaagt gcttttaaaa tctcctttga gaaaaaatag
     6241 agatagagag acatatattg aatgtgttgc actactacca gagaaaagat ttgacagacc
     6301 tttagactta gatagtttta acataatgct acaatcagca tttataaaga ataatgatag
     6361 agatacactt ttaaaaattg caggtaatat ccaagaatct acagtaaaaa acgtgggaga
     6421 tgatggagtt tctcaatctg taacaataaa aacaggtgtc gctagtgtat cagaagcaat
     6481 agttccaaat agagtaaaat tgattcctta ccgtactttc caagaagtag agcaaccaga
     6541 aagtgaattt attttcagaa tgagttctgg accagcagca ggattatatg aagcagatgg
     6601 tggagcatgg aaaaatcaag caatgttaaa tattaaagcg tacttgcaag aagaattaaa
     6661 agattttaaa aatgtaaata taattgctta attgaaaatc taagaggact tattataagt
     6721 cctcttagta aaacaaggag ggagttaaat gaaaagtatt tctcatatga acattttaga
     6781 aagagcagaa tttacagaca aaattgctaa tgcaattatg tctgcaaata aagaaattga
     6841 aagaggttgt gcctttaatg aagcagttga gattgtaaaa caaatggaat atagagaaaa
     6901 taaatacaat gaaggagata ttgcaatatg aataatgtag ttttagttgg aagattagca
     6961 agagatccag aattaagata tataccagag tatggtactc cagttgctac ttttgcattg
     7021 gcagttgata ggggttatgc aaaaaaggat ggaactagag aagttgattt tataccaatt
     7081 gaagttatgg gaggatcagc agaattttgt gctaattatc tcacaaaagg aagaatggtt
     7141 tcaattcaag gccaaataag gatagaaaaa tatgaaaaag atggagagaa aaaaactttc
     7201 acaaaagtaa gaacaaaaat tgttaatgcg cttgatcaca agccaaagga tgaagaaaaa
     7261 gaaataggct ttcaagtttt agatgatgaa gatattccat tttaaattgg atggtgatta
     7321 tatgagaagt ttagaagaga aaaataaatt atttgaagat aatataaatc ttgtacattt
     7381 tattataaat agatatttca aatccttttt aagaaggtat ccatatttaa aagaagattt
     7441 atttcaagaa ggatatatag gattatataa aacaacatgt tgctttgatg aaagtaaagg
     7501 aaaattttct acaatagcat tttcgtatat atcaggacat ttgaaaagat ttacgactgg
     7561 atatgttaag aaacattata gaaatgatat agatagtttt gaaaagtgta tttatagaga
     7621 taattatgga gaagagataa gaatagaaga tagattggct ggcaatgaaa atgtagatat
     7681 agaaaatgtt cgtgttatta gatcttgtat aaaaagaagt gagataaaag atatacagaa
     7741 aatagtttgt ctaagagaaa aaggttatac tcaacaagaa ataagtaaag tgcttggaac
     7801 tagccaaacg tcaatatata ggagacttaa aaaattaaaa actgaaatta atatactagg
     7861 taaataataa atgatttaag tttattggag gtaaaatgaa aactaaaata attggtggta
     7921 gggaaaatga gtgtccaatt tgtaatggta atatatttaa gattgaaaca ttaattggaa
     7981 tagtgtggca atgtaaggat tgtggatgta tgtatcaaga tatgagttct aaagaaagta
     8041 aaagggggta ttaaagatga gttgtgttat aaagtgtgat ttttgtggtg aaacaatagc
     8101 agaaattcta aacataaagg tattcagaat tgatgatgaa ggtaatataa tgagtatcgg
     8161 aaaagatgta tgtgacaaat gttatgacag attatttaga agtaacttga ataagaagat
     8221 gactaattat gaaaagataa aaaatatgag caaaatagaa atggctgaat ttcttgcaaa
     8281 tggcggaagt ggatgtacta gttgtgctta tgattttcaa gaatgcttag gcggatgttt
     8341 agaaggtaga aaaaaatggc ttgaaagtga ggcgatgaaa taatgaaaaa gacgttaata
     8401 atcgtaggag tagttatttc aatattaatt atgtttgctt gcttgttctt agtaactaaa
     8461 aataaagcaa ttgatttaga agagcaggtc caatcagcaa ctgcatctgt agaggtgcaa
     8521 gaaaaaagga gaattgattt aattaagaac atagtagact gcgtaaaaga ctatgctaaa
     8581 tatgaaaata gaacattaaa agaagtaact gacatgagag taagtgcaaa tgatgaatct
     8641 atcaaggaag caaaattaaa tatacaagca gtagcagaga agtacccaga tttaaaagct
     8701 aatgaaaatt ataagcaact aatgagtgag ttgagtataa cagaaaatat gattgcacaa
     8761 catagagaaa attataatat gcaagttcgc aattacaata aatatataag aaaatttcca
     8821 gcaaatttaa ttctaaatag tatgggatat gaaaaaatag aatctaaata tactgaatat
     8881 aaagcaaaag aggatgcacc tcaaaattta tttgattaag aggtgcacat atgaaattta
     8941 aaacttttga aataactaag agagaatttt tagtgagcat aataattgct tgcatgatga
     9001 ttgtgcttgg atttttcatt gatgataaag ttcaatcaaa atttttagaa gataatgaaa
     9061 gatattacaa agcaactaaa ataaataatg acaaagatct ctttgattat gctatggaaa
     9121 caagtgcggg agattctttg ataagtggaa gatttaaaag tgtagattct gtatctatag
     9181 aagaattaaa aggggactat tggtttatag aaagaacgga agaaaaatac actatgcata
     9241 ctagagtggt taccactaaa gacagtaaag gccattcaca taaatatact gaaacatatt
     9301 ggacttggga ttaccatgat tcaatagaga aaaaatgtaa taaatttaat ttcatgggta
     9361 aagagtttga tgcgaaagta attactaatt tatctaaaga gagattggca ttaaagaaag
     9421 atatatttaa aaataataat cttaaaataa aaggtaatta tgcttacatt aataataaga
     9481 gaaggtacta ttatgaagtt ataccgaaag aatttaatgg tactatgtat gctaatttaa
     9541 aaaataatac tatagcgaat ccagctgata aaaacataaa agtttttaag ggtgagtcta
     9601 tacagaatgt tttagaaaat aataatttta atacagggtt ttttaaagta gtgttttgga
     9661 tcatctggat attaataact acaatcatta tatatgtatt ttattattta aaaaatgatt
     9721 ggcttgaaga taaataaaga tgtaagagga gaagatcagg tatgaaatgt aaggtgtacc
     9781 atgctattaa aaatataaaa gatgcaaatg agttaattga tagatttgaa acggaaaata
     9841 tattctttga attaaataac atagataatg aaaatgtctt aaatggagaa atggatttaa
     9901 ctgatataga agatattgca tcattgcaat ttatattaga tggagattta tttatattta
     9961 gtagttgtga tgaaatgata tttatgaata aatacactca aagggagttg ggacttcaat
    10021 aaatgaataa gagaatagta tgtcaagata caaacgagat tgtctataat tataaagaat
    10081 atcaaaaaac taaacactgg gaatcattaa aaaatgaatt ctatttttct aatgaatgta
    10141 aatgtgtttt gtgtgattca gtaaagaaag tgtctttgca tcataaaaca tatcgaagaa
    10201 taggagcgga agaattggat gatttaattc cattatgcaa tagttgtcac tataaagttc
    10261 atcgaaataa taaacaatat ctatttaata aggcaacgga aaaacagata agatgcataa
    10321 atgatttatt ggaagtttat gtagatattg tggaagaatt atttatgaat gaatatattt
    10381 atctttatgt tgccatggga atagaatttt acaatgaaaa tctaatgtta tttgaagcaa
    10441 atcatattat caatgatctt aagtttgttt ctgcaatgct gtatgagttt tatgccacaa
    10501 agttggaaat aaaacttcca ggatatttca atggatttga ctacgaagaa ataaaagatt
    10561 ctttatataa aaagatgcag cgattaagta aatatattta tgttcccaaa aaacaagaga
    10621 ttgagttatt atttaaaaaa agaaaaaagg agcattatga ataaatatac tcaaaaagag
    10681 ttgggacttc aataaatgaa tgaggggaga tactaaagat ggatttaaag ttaattagca
    10741 gcatacctaa ttggaaggta gaagatcagt tggcaaaaat tgatgaagag gtaactgagt
    10801 ttaaaaatgc tatagataca ggaataacta aagatataat tgcagaagga ttagatgttt
    10861 gtcaaactat tttaacaatg tttcaagttt tagaaataga aaactatatt actgaaggag
    10921 tagagacaca taataagaaa ctaaatggaa gaggatggga tctcaaagaa ataggagacg
    10981 atatacttaa tttaattaaa aattcaatga ttaatgaatt gattaatatg aacaagaaat
    11041 taaaatgcct tgatgaaaaa gaatttaatg caaaaataca atttttagat gtagataaac
    11101 tatggaattt gtgcatgcct ttagttaact taagtactat aaaaggagtt aatgaaagca
    11161 ttgatgaaat gaaaatggga gagtttctaa aaaatattaa ttaaagtaag gagataatat
    11221 aatatgttga tagaagtaac tgaaaataat agagaattaa aagtaggaga tattgttgaa
    11281 attaatggtg gtaataagtt atatatgatt ctttctttaa aggaaacaat aggtgggtat
    11341 gttattataa atatgcagaa tggatatggt tcatttggtg cttataaaag tttaagtcaa
    11401 ctagaattag acttgaaggt aagaggttat aaattatata gttctgatga ttacaagttg
    11461 caattagtgc ctagagaata agatatattt caaaaaagaa aaaaaggagc attacttcac
    11521 actcctgctt gtcaaaattc taaaaccgtt atcacaacat cattataaca taattaggag
    11581 tgtgaggtaa tgcaatctag caaaaaagac aatttgttta gtagtgcaga aggtaagtta
    11641 tataattata aaaaaataaa agcggaaata gaaaaaatag acattgatat gcagatgata
    11701 aaaaacgact acagaggatg tagtggagtt gaaattaaag aaaagacaag caagacatat
    11761 gatattaaaa gtgtaattga aatagagact gaagaaaaag aaaagaaaat tgccttaaaa
    11821 gaaaaagaga agagacataa agagttgata atagaaaaaa tagataatgc aatgaaaata
    11881 ttaagtgaag aagaaaaaaa gatagtacag tataaatact tttcaagtag caggacatct
    11941 tgggaatatg ttggcagaat gattggtttt tcagcgagta agtgtaagca aatgaggttt
    12001 gatattatag ataagataaa aggattgcta taaaaatgtc cgatttgtga cgattttaaa
    12061 ccgatttatg tctgttttgt gtctaaaaaa tgtccgattt gtgaccaatt tatgtctgtt
    12121 ttgtgtcttt taatcatgat aagattgtat tgtgagaaaa atatatttaa taaaaatggc
    12181 ttggaattta cgttctaagt cttttttatt gtctagagag gtgttgaaga ttaaacgtga
    12241 tataatagaa gaaatttcaa gtgctgcata tgtaccagac aatctaaagt attacgacaa
    12301 tgtaatgaga gaaactcctg gtatgtatgg aagagaatgt tctttagatt ttataaagaa
    12361 aaagcaagaa aaactcttga aactaaaaaa gaaggttaag aaaaattatg attctaatat
    12421 taataagata gactcatatc taaaaatttt agatgaaagt ataattgatg agtctgatca
    12481 cgttgagttg gttgtattta aattatattt gcaattagaa aatgttgtaa aagtaactga
    12541 aactgtaaat gacttaggat ttagaataaa gaccaatact tatgcaagag aaagaaaata
    12601 tagtacaaat gatataacct ctataatcac agatcctttt gctaatgtag ttgaagactt
    12661 aaaaatattg gtgcaagata ggcaaagaaa aaattatcat ggtaataagg agtgttttta
    12721 atgaatggaa gcacatagtg gacaaaaagt taatccaata aaagatatag aggatgtatt
    12781 tagactcttg aattttctag aggaatggaa cgaaagaaat tacttattag ccctctttgg
    12841 aatgtgtact ggacttagaa taggagatat attagcactt aaagttgcag atgtaacaga
    12901 tataaagtta gataaaaaag gaaaaaagat aagagtgtct aaggactgga ttagagttat
    12961 agaagaaaaa agagactatg atagagaagt tttcttatct gatgtaataa agagtgctat
    13021 agaaaactat actcaagata aaccaggaga agaattttta tttaaaagtg gcaagaggaa
    13081 gaaatttaat agacctatcc aaacaagaca agcaggaaga ataataaaag gagcagcaaa
    13141 aaaggtagga ataaaagaaa atgttgcaac acactcttta agaaaaacat ttgcaaggca
    13201 tatttatgat gaagaagaga ataagactta tgcattagaa cttataagga agattctagg
    13261 acataagaca atagaaatga ctagaagata tataggaata gataaggatg aagaagtaaa
    13321 agcaataact aaatttacta ataagttaaa gaaaagaaaa agaaattaaa atatcttgta
    13381 ttttatctaa aatttaatat gttacgtttt tcaacaacag gacattatta gaatgcaaag
    13441 agccttaaca atagagttta caaggaatag aatagattta ttaaaatgtc tgaaaatata
    13501 tattaaaggt catttattca agggatttat agggatgtag atatttaaat gtttatttag
    13561 aagctgagta aagaggtgat taattggatg tgaatgaagt tgaaataatc gacagtagct
    13621 atgaaaaagg taaagagttg acagaagatc agtgcaatat ggtaactcta ttgattacag
    13681 gtactacagt aacagaaact gccagaatta ttggagtaaa tagaaaaaca atctataatt
    13741 ggatgaataa agagcatgtt aggaaagaaa tggacagacg gaaacaggag ctaacaaacc
    13801 aagggaactt aatgatatta aaggatttag atagttatat caacaatatc aaggaattag
    13861 ctagtgacaa gagcgataag agggttatgt tggcagccaa tcagtatctt ataaatcgta
    13921 tatatggaac tcctacaagt acagttatac aagcagagga taatagtgtt ggcatgggta
    13981 tggatgcaac agagatagaa gcagctatag ctaagataag aataagaaca agcaagaagt
    14041 aattagatat gaaataaatc ctgcaagaat atgttttaat tatgtttgaa attaattaaa
    14101 gcaaaacata tattatatat atatttactt taataattaa atcatatatt gtatttaact
    14161 ttagttatag ctaatttaat aaaatttagt gagacttaat aattaaagtt tcatacagtg
    14221 aagtgatgtg gattattata ggatatgtga acctattgga aatgctaggt tctttttaac
    14281 tatgccgaaa atacatattt aaggaatagt tgaaagataa ttaataattc taatcaaaaa
    14341 aatatttctt gcaagaaaat aattcaaaat actttaatat atttattttt tttattaata
    14401 gggggtgtcc ttctattttg aaaaaattat tttttatgga aaatgagttc tagaaatttt
    14461 ctagcaaaat ttaaaaacca gagatgtatt tcaaagaggg tgattttatt agagaaaaat
    14521 ttacattgtt tattttatta attgcattac tcactgagcc taaaaaaata gaagaagtaa
    14581 gaggaagtaa atacgagtgg ataatctaga aagaaatata caattaatat ttagttatct
    14641 agcaaagact tttataagaa atggagcatc tataaaagat gctgaaaaag aagcaaatga
    14701 gattataaaa gccaattcaa acaatctttg gggagttaat ggtctcgctt atcaacttgg
    14761 aaaaataaac ctagaatttt tctgtatgta ttttatgcaa gacacatatt taccaaaaga
    14821 agataatgct gctgctccta tagctaaagt tcatcatgag ctatgggaag atatacaaga
    14881 atctataatt ggagatggtt cacagcaatt aggaagaatt tatccaagag ggacaggtaa
    14941 aagtgcattt ggagatttag cgacaactgt atggtcgcat tgctataaac ataagacata
    15001 tactttgatt tgctctgaca taggatctac agcagagaaa tttgtaaaag atataaaaaa
    15061 tgcattactt gaaaatgagt atattaaaaa agcatttggt gtccttttaa atgataatga
    15121 tagaaaatac atttgtaata gtactcaact agagctaaca aataaaactt ttattgaagc
    15181 tatatcatct tcatcaccca tgagaggaag aaaatacaat aataaccgtc cagatcttat
    15241 catacttgat gattatcagt cagaagaaaa tgttagaaca gaagatgcta gagagaaaaa
    15301 atttaaaaga ttttctgatg atgtaaaata tgctgctcaa aaaccagtca taagaaatgg
    15361 taaaactata aaaagaggaa caacttttat agcattagga accttacaac acaaagaatg
    15421 tttttatagt agactaaaaa atttaccaac ttggaagttt aaatgcgaaa aaggagtttt
    15481 agttgataat gtagatgaat tatttaattc tggtttatgg cttaagttca aagagatctt
    15541 atttgatttt aaaaatacaa atcatcttga agatgctaaa gaattttact gggaaaatga
    15601 aaaagaaatg aaattcccta tattatggcc aagtttttgg gattgtttag atatggctct
    15661 aagttattat gaaaatccaa catcttttaa gcaggaagtg caaaatgatg tcaattctat
    15721 aggagaaaaa tggtttaaaa ctgttagaac agaaactaga gaagaaattg aatcgcatac
    15781 ttttaaaaag acaatgctgt tatgtgatcc agcatctgcg ggtgggtcga aacatgacta
    15841 tagcgctttt cttgttggaa gtgaatcaga aaatggttta ctatatggaa gattggcaga
    15901 actagctaaa ataaatgcta gaactgattt tgataaatat atagaccata tgatttattt
    15961 attaaaagtg tattcagata taacacatat ttatatagaa aaaaatacat ttaatggcgc
    16021 agatgccaac caattagagt ttaaaataaa aaatgataat gttcttagtt atagagatat
    16081 agaaatcatt aatgagcaac aaaagaaaaa taaagatgat aaaatctcta ctttaatacc
    16141 tgttttaagc aaaggccaaa tgatttttgc agaagaagat aaggaattta tacagcaaat
    16201 acttgatttt acaggacaaa aatactctct acatgatgat gctccggata tatcatcgga
    16261 atttattaat cgaattaaca acataaaagt ttctgagaca gtaaatatct ttgatagaaa
    16321 attattaggt ttttagttgg gaggtaattt attggctaat agatcaaagt ttgaagaagt
    16381 tcaaaaaact ttaactaaaa aaggaaagaa gttcaatgtt ggaataggaa aagatgaaaa
    16441 aggatatttt gcatatactc atagagcaag gagtaaaagt tatatttcaa agcaagatat
    16501 tccaataaaa gttttaaaat ttatagagtc tacaggatag gatgtgttgt taatggaatt
    16561 agatttagac ttaataaaag acatatatga cgattggaac tctaataaat ccgaatatga
    16621 tactatgtat aagtattata aaggtgaaac ggatgctatt agcaactata aaatggttac
    16681 caagagatct aataataaga taaacactaa tttcttaaaa aagtttatta atgaagaagt
    16741 cgcttattct cttgcaaata agattaccta tacaagtaga tctggtgatg agaagattat
    16801 aaatgattta gaatactata cttgccattg gagtaaaaaa catgactcag atttattgag
    16861 gtatgcacta ttatttgggc tttgctatga actttactat gtaaaagatg atgaaataca
    16921 agtaaagata atcaagccaa cagatggctg tcattatgaa aatgaaaatg gagaaatagt
    16981 atgctttatt agagagttta ccaaaggttt cgaagatgac acgtatgtag atgtatatga
    17041 caaagaatat atatatcatt ttgattcaaa tttcaaagaa gttgaaagtc ctacagtaaa
    17101 caatatattt gatggaaatg ttccaatatc aatttgcaaa agaagtgaag aactaggaaa
    17161 aaatacaata tttaatgata taaaagggtt acaagatgct tatgaaacta atttaagtga
    17221 tataagtaat gaaattagtg attttagaaa tgcttattta accttttctg gatgtaatat
    17281 taaagaagat gatttgccaa ggatgaaaga acttggaata cttcaagtta atggagatgg
    17341 aaaaatagag tggcttatta aagatatgaa tgatacattt gttcaaaata cactatcttc
    17401 aataaaagaa aatatgtatg aaattacatc tcatataaat cacaatgaaa aaatgcaaag
    17461 taatacatca agtctagcag ttatagcaag attaatcagt acagaatgga tttgtagcca
    17521 aaataatgat agtattgcag atactttatt taataggtac aaattattgt gcatttggct
    17581 taataaaaaa tatggttttg actatgatta taaagatatt aaggctaaat ttacccctaa
    17641 gataccacgt gatgatttag ttgtagcaaa tatattaagc caacttggag ataaattatc
    17701 tactgaaact ggcttatctc aactaagttt cattgataat cctcatgctg aaatagaaaa
    17761 agctaaaaaa gaacaggaaa tagtttcaga gggagagatt ttattagatg aatcaaaaga
    17821 ttataactaa attgactgaa gatatatata aatctgctga aaataggaca aaaccatttt
    17881 ataagtatca aagagaaaat agggataaac ttttattaga aatagcaaag atattgctaa
    17941 cacacgatat tattaatgaa tacttaaata ttaataataa agataagaaa aatctaaggt
    18001 caaaacttaa taaaattata aatgagtatt ctaatagtta tgatgaagaa gtcaaggata
    18061 tagaaactat tttatctgat tcatctaaga ataaatattc aaatttgata attttattag
    18121 gagtaatact gaagataaaa gatgttagta atataaacaa taaagttata aataagatag
    18181 tgaataataa ggttgatgga aaacattgga gcgatagact ctggaagaat aaaaaaggta
    18241 tagaagagtc cttaaaaaaa gaaataaaga gttttctaaa tggaaaaact tctgtaaata
    18301 agatagaaaa agcaataaga gataaatatt caacaggtgc aggacaaacc agcagactaa
    18361 tagaaaatga agttgccaga tgtcaaagtg aagtaaatga gtattttttc aaggttcaag
    18421 ggattataaa agtaatgtat tgtgcagatt tagattctcg cacatgtagt gattgttcta
    18481 tgcataatgg agtgatattt tatgtcaatg aagataggcc ctctttgcca agacactgtc
    18541 actgtagatg tcaatatata cctgtttaac atgtattaat taatttaata ggtgttttta
    18601 ttatgtctaa aaaatatgag gtgaaatatg aaagttgaaa tattaggaac agaatatcta
    18661 gtgatagaga aacttgaaag cgaagatgtg ctattaaagg aaagagctgg atattgtgat
    18721 cactctgtta aggaaattgt tatagagaaa ataaattctg aagaaggttc attgaaagat
    18781 ttaagtgtat ataaaaatga agttgctagg catgaaataa ttcatgcatt tcttagtgaa
    18841 agtggattga agagttgtag ttcttgggct acaaacgaag aaatgattga cttctttgca
    18901 attcaatttc ctaaaatagt aaaggttatg gataaaatag aatgtttgaa ttaaattttc
    18961 aaaaaggaga actttataca tgttaaaaaa agaagtgttg gaattactaa aagaaataga
    19021 agatgatgca gaaatagatt cattgctgca atcaacagat ttattcaaat ctgcatctga
    19081 taaaaagctt acaatagaag agtttagaga attaattaat aatgatgcaa actttaaagc
    19141 tattatagat aatgaaaaaa ataaatatca cagcgaagca cttgaaaact ttaagaaaaa
    19201 agatatgcag acacttataa gtgctgaagt tctaaaaaga actggagcaa atgaaacaga
    19261 agagcaaaaa gcaataaggg aattaagaga aagcctagac aaactaaaaa aagaaaagca
    19321 gtacgcagaa aaagtttcta aatataaaga tattttagtt gaaaagaaaa tacctacaaa
    19381 cttaattgaa tatctattat ctgatgatga tgataaaaca aatgcaaata tagagatatt
    19441 tgaaaattca atgaagcaat atgtgcagtc aaaggtagat gaaagaatta aagatggttc
    19501 atacgttcca cctggcaaag atagtgcagg ttcgctatca gaaattagaa aacaaataaa
    19561 gcaagggcta aatagtcttt agtataaaaa aagaaaggat gattttataa atggctaatg
    19621 tattacaata tgtaacactt tttcaacaag aactagatat gcaggtaaca gttggctcaa
    19681 ctactggatg gatggagaaa aatgctggtc aagttatata taatggagga agagaggtaa
    19741 aagtaccaaa agtaacaatg gatggcttag gtgattatga cagatctaaa ggatatacgc
    19801 aaggcgcagt tacacttgag tacgaaacaa tgaaaatgga gatggataga ggtaggactt
    19861 ttatgttaga ttcaatggat gtaaatgaaa gtaacttcgt tataaatgca tcttctgtaa
    19921 tgggattatt tcaaaagcaa catgttatac cagaaataga tgcatataga tactcaaaga
    19981 tattttctga aacagctaaa gtagatgagc gtgtaaaata ttcatacgaa gctacagtaa
    20041 ctacaatatt aagtgaattg aaaaatcaac ttgcatcaat acaagattta tctggagata
    20101 tacctcttat tattagtatg tctagcataa ttgcaagcat attagaacaa tcatctcaac
    20161 ttgatgatat tgaatttggc gatggagata taaaaagaat tgtaaagtct atagacaatg
    20221 tacctataat aaaagttcca tcaaaaagaa tgaaaacaga atacgtattt agagatggta
    20281 aaacatctgg acaaacagag ggtgggttta ctgaaggagc aaaggcttta gatataaact
    20341 ggttaataac tccagcatct gctccaattg cagtatctaa gactgataag ccaagaatat
    20401 ttactccaga tcaaaaccaa acagcagatg cttatcaaat agactataga aaatatcacg
    20461 atttgtggat attggatgaa tatttaaaac tttgtagagc atctgtgaga caagctaaag
    20521 ctgcacaaga ataaggagtt gttttaaatg tttgcattga taaaaaataa cataattaaa
    20581 gttgttgaag atgaaaattc gagagataaa ttgatttcaa aaggatttaa aatatattca
    20641 gaagaagtga ctaaagagaa tattgagaaa aaagaagaaa aagttaaatc aacctctaat
    20701 gatttattta atctatctaa accagagctt atggaaattg ctaaaggcaa gaatataaag
    20761 ggatattcaa ctaaaactaa agaggaatta attcaactac tagatgagta caatgcttga
    20821 taatgttaaa ttagttttag gaatagaaaa tgatagatat gacaaattaa taatccttta
    20881 tataaataaa ataagtgaga tggttttaga atattgttct aggcaggaac taagtatagc
    20941 actagaaggg tttgtagaag aaaaagtaat atcaatatta tcttcaagat ttgaagaact
    21001 taagaaaaat agtggacttg ataatctaga aaaaagctta aaagctataa ctagaggtga
    21061 cactaagata gaatataatg ctttaacatt acaaactgct gaaaatagca tcagttcatc
    21121 atctttttta actgatactg ataaaaaatt tctaagccca ttttgtattg ttagtatgta
    21181 ctaaggggtg attaaatgac tgaagcagat atattagaaa tgacatacct tgataaaatg
    21241 actataatta gaaaattaaa aggctggaat gaagaaacat gttctaatga ttttagtaat
    21301 gttgttatag ctgaaaatat cccatgtgca atttctagaa aagaagaagc cattgtaaat
    21361 ggtgatattg gatctatagt tgtaaataga aagttatttt gtagaccaga agtagatata
    21421 aaaataggtg atactataaa tacaacctat aatcatggag aagaagctgt atttaaggtt
    21481 tctaaggcaa atatatatcc aagccatatg gaaataccta ttacagaatt ggagaggata
    21541 taatgggaat agataattta ggagtctttg aagcattagc cttagattta ggtctaaatg
    21601 catcaaactt taatagaaaa atcaagaagt ttacaaaaga aactgggcaa gaactagcag
    21661 aaaatataag agatgaaact cctgtaaaaa ctgaaaagtt aaaaaatgga tgggaagaaa
    21721 agcaaccaga atatgatact tatattgtta aaaatgaagt tgattatgct actttagtaa
    21781 ataatggcca ttcaactaaa aatggtggca ttgtccctgg ttcttttatg gttgaaaagg
    21841 gaattgaaag aacaaaagaa aatatgacaa gtgcaaaagc aaatttattt gattggtagg
    21901 tgattagttg cttacaaaca gagacattat aatagcagtt aataaaagaa tcgaaaatga
    21961 gtttagatat atagatattt ttattaatga ggataatata caaggctttg aggaggcttg
    22021 tttttttgtg caacttttac ctgtttctaa taatattgca aataaagttt tagatactaa
    22081 aaagatattc atagatattg aatattacca aaaggctaga aaaaataaat taaatctata
    22141 cgacatacaa agtagattag aaaaaatatt taacagaaac attagagtta aagatagaag
    22201 tttaatcatt gagaatatat ctccatcaat cgcaaaagat cctgtaggat ataaactaac
    22261 ttttctaatg actgtatctt attttgaaga agtctatttt gaaaatgaaa catttgaaat
    22321 tatggaaagt gtagatttag agataggaag tgattaaatg ggtttaccag aaattaatat
    22381 ttcttttatc caagcaggaa aaacttttgt taaacgctct ggtggtattg tagcgcttat
    22441 tttaaaagat acaaaaaata tagggttagt tgagataaaa gaaatagagg atattccaga
    22501 aaattattct gctgctaatt tagattatat aaaaatggct atgaagggaa atgtatatcc
    22561 tccaaataaa gtattagttt atacattaga tacagatgaa tcaatagatg atgcactaga
    22621 ttttcttgaa acttgtgagt ttaattatct ttgtatgcta gatgagacag aacaagattt
    22681 gcctaaaatc aaaacgtgga ttaagaaaat gagagaagat aataaaataa aggttaaagc
    22741 tattactgca agtaattcag cggattatga aggtataata aactttacag caactgatat
    22801 tgaagttgaa ggtaaaaaat acacttcaaa tgaatttctg ccaagaatag caggttttat
    22861 tgcaggaact ccatcaactc aatctgtaac atatgcagaa ataccagaag ttactaatat
    22921 acctaagcta acaagagctg aagcaaatac aaggataaat aatggagagc taattttaat
    22981 aaaagaaagt ggagctatag taatcgctag aggagttaca tcctttacaa caattactga
    23041 ttcaaaaggt gatatattca aaaagattaa gttggttgat acactagatc aaattcataa
    23101 cgatattaaa aaaataattg taaaaaatta tataggaaaa acccaaaaca cttatgataa
    23161 taagtgttta cttattgttg caatacaatt atatcttcaa gaattagaaa aagatggttt
    23221 aattgatgaa ggttcaactg tagaaattaa tttagatgct caaagagcat ggctaaaaaa
    23281 acaaaatata gatatatcta atatggaaga acaacagata aaagaataca atactgacac
    23341 tcttgtattt ttaaaagcaa gaattaaatt aatagatgca atggaagatg tatatttaga
    23401 catctctatg tagtttggag gttaaaattt gaaagctaaa atagatgcaa aaagaataat
    23461 atctggtact tttggagagt gttggctaga taataagcaa cttacatcaa caaaagggat
    23521 ggaagccaag gttgatatag ataaagaaga aataaaatta tgtggtcaaa taatgaccac
    23581 atataaaatg attggtgcaa ctggaaaagg atctataact ctttataaga taagtacctt
    23641 atttggcgag tatgtcgaaa aaatgttaaa tgaaggtaaa gaatttaaag taactataat
    23701 aagtaaattg caagacccag attcatatgg ttctgagaga attgcacttt atggatgtat
    23761 gtttgacgac ttaactttgg ctgattggga agctgcgaaa gttggagaaa tagaagttcc
    23821 attcacattt gaaggctttg aatacttaga taaaataaaa gaaaaatagg aggattttat
    23881 tatatgaaca cagtagaaaa attattaaat atggatgcag gtaaattaaa aatgccatct
    23941 agcgcatttg aattatattg taaaaaacta gatgatactt tagaaataga gtgcaaggct
    24001 attgatccag aacgttttga cgaaataaga atgaactcta tggacgttaa tagtggagat
    24061 gtagaaaaca ttaacatata tgaacttaaa gtaaatacaa tattagaatc atgttctata
    24121 tttagcaata tggaattagt taaaaaattt agtgcaatta cacctaaaga attaattaaa
    24181 aagctattac ttagtggtga aatagataaa ttatacgagg aggttaataa agtaaatgga
    24241 atagatgcag agggcgataa aaaaagacaa aaagaaagag ctaaagatat aaaaaactaa
    24301 ttaaaacgga tggagaagtt aacttgatgt acttattatt taagtataaa ggtataatgc
    24361 catccgtttc ttatcgtata ctaagaggcg aaaaagaaat tgttagtgtt tttatggatc
    24421 aagaattaga tgaaagagaa gaagaaataa aatctggtgc tgggaggttg ttttagtata
    24481 tgagttctaa tacaaaagtc ctacaagcaa taataaaaat gagagatgaa gcaagttcaa
    24541 cattaaaaag agtagcagca aatactagag ctctacatga ttcatcttct caagcatcaa
    24601 gaacattaaa taatgttcaa agtaatttaa aaagcgttgg aactagtgct ttaaaagcag
    24661 gaggtcttat cactgctgga gttggtggcg catttgcagc tgcaactaaa gttggaacag
    24721 aatttgaaag cgctatgtcg caagttaagg ctacatttgg aaattcctta aaaccaggag
    24781 attttgaagc tctgtctaag gctgctagag atgccggaaa agctacaact aaaactgctg
    24841 ctgattctgc taatgcactt caatacatgg ggcttgctgg atgggatgtt caaaagtcaa
    24901 tgcatgcctt aatgccagtg cttaatttat cagaagcagc ctctatggat cttggtctag
    24961 catcagattt agtaactgat agcatgagtt ctatgtgctt agaagttgat aaggtcagta
    25021 aaagtggaaa agtattagcc ggtgcaggtt taagtgagta tttagataaa gttgcaaatg
    25081 catctactaa atcaaataca agtatacaac agttgatgga agcgtttaat gttgcaggtg
    25141 gtacatttag caatttaaaa gttccgcttt cagaagcaac tgctatcatg ggaatacttg
    25201 caaacagagg agttaagggt acagatgcag gccatgcttt aaactctatc atggtaaatc
    25261 ttacaacagg tgcaggccaa gcaggtaagg caatgaaaaa gctaggcgta agtgcatttg
    25321 ataatcaagg taaatttaaa ggtatgtcaa atgtacttaa agaagttgca aataaaacaa
    25381 aagatatgac tgataaacag aaaaattatt acttagcagc tataggagga aaaactcaat
    25441 taaaaacgtt acaaaagtta cttgatggtg tgggaaatga atatgatagt ttaaagaaaa
    25501 aaatagataa ttcaaaaggt gctttaaaaa caatgtctga tgtaatgaag gataatacta
    25561 agggtggact tgcaacctta aaatcagccc ttgaagaaac tggaattact atttactatc
    25621 aactaaagcc agcaatagca tctgtagttg ataatttaac taaaatgact gtttggttta
    25681 ataatctaag tgaaccaata aaaagagtca taactaatgt aatgctttca gttggagcat
    25741 ttggagtact tttattaact ataggggctg tgtctttaat tgtttcaaaa gctataggta
    25801 tatttggctt ttttgttcca ataatattaa gagcaaaagt tgctttcaga gcttttgtaa
    25861 ttggtatgaa accattaagt gcagtatttt tagctgtaat gggtcctata ggatggacta
    25921 taacagctgt aattgcattg tcagtagcat ttaccattgc ttatgctaaa tctgaagtat
    25981 ttagaaataa ggttaattca ttagtaaaat catttacaga atttgcaaaa cctatagtaa
    26041 atttttgtat gcctgcactt aaacaattag gggaaaaatt taaagagttg ttatcagcat
    26101 ttggtccact atttgcgtct attttaaact taggaagtgt aataatgaag gtactaggac
    26161 ctgttttaat tggcgtaata ggttttcata tagcaaaatt agttttgatt ttatctactc
    26221 tggcaactgc ctttaaagct gtagttgata ctataactaa tgtattaaat ggtctagcat
    26281 ctattttaaa tggagtattt aatttaatta taggtattgt cacaggcgat agtaaaaaga
    26341 tgatgaatgg agttaaacaa atatttgatg gtggagtaaa aattataaaa tcaatttgga
    26401 aaggtttcat agattttgta actgctccta tacaagctat agctgatata gctgacagct
    26461 tatttaaaga taaagctaaa tggcttacag ataagtggaa agatttaaga acattcttag
    26521 aaaatccaat tgaagcaact gccaaaatat ctactaaaac aaatgtaaac attccaggaa
    26581 gtaaagcagc tgatgcagct gggaaatcta caccaaatac aggtggatta aaacaaacaa
    26641 tagcaaatct aaaaggttca tttagtgatt ttaaaacttt tattaaacct atgacagatt
    26701 ctataagtca aggagttgaa tctggaaaaa ctaaatttac agaactgcaa aacaaaatat
    26761 taaatgatgt taaacctgca ttaaaggtcc tagaggattc tggagtaaat tttaaggata
    26821 agtttaagga gtcaataact tcaataaagg ataaatttaa tgaattaaag aacatattag
    26881 ctggaaatat taaaacaaat atagataatt tagtaaaagc atttgaacct ttaaaacctc
    26941 atttagataa tttgaaagag tgttttgata aggttaaaaa agcgattact gattttttta
    27001 ctccagccaa acaggttgga gatacagtaa acaaagtaag ccaaccaatg gatagtgtta
    27061 aaaagtctac tgatggaatt aaactttctt tccaaagttt aactcaagca cttgctcctg
    27121 ttaaagcttg gtttcaagga tttatgcctt tcctatcaca aataggacaa caacttttac
    27181 catctatagg aattgcaata gctggtgttg ttggcggaat aattttagca tttacatggg
    27241 ttttaaatac attaaccgca ataataacaa cagtatctgg aatcataaat ggaattatgt
    27301 cttttatatc tggaattatt aatgttatta tggggcttat aacaggaaat gctgatcaaa
    27361 ctatggatgg acttaaacaa atatttgata gtggagtagg tgtaataaaa tcaatttgga
    27421 atggccttgt tgatctagtt agtggtgtac tggaacctgt aatagatctt gctgactcat
    27481 tttttaaaga taaagttaaa ggggtaaaag aagcttggaa taaacttaaa gatttcttta
    27541 aacatccaat taagggaaca atagaaatat ttaaaaaagg tcctagttta caaattggaa
    27601 aaggtgcaga tgctgctggc aaaagtccaa gaaaagcatt tggtataaat agagttccta
    27661 gaaatgatta tttaattagg gcacatgaag gagagaaact tttaaccaaa caagaagcca
    27721 atcaatacaa aagaaatagt tcaaatggaa gtttatttct tgataaattg gctgatacaa
    27781 taattgtaag agaagaggct gacatagata aaattgtaag aaaaataaac aaaaaaatat
    27841 ctcttgcaaa agttggaggc gttttgtaaa ttatcgtgct ataattgtag tatataattt
    27901 acagggggtt atgttatgaa taaaaaaata ccagcattag caatattaat tgtattatct
    27961 ttaagtttag ttggatgcag cacaaatgat tcaaaaaatg gagatagtaa acctaagcaa
    28021 aatacagagg aaattaaaaa tcataaaata ggcgaagttg ttgatttgga cggagcaaag
    28081 gtatctgtta aaaatgttag aaaactagaa tcagatagta atgtatatgc agttgagatt
    28141 gaaatcacca atacaggaga tacagatctt gaaagtgatt tccttcaaaa ctacatacta
    28201 aaaacggatg ataatacagt tggaaatgca ataaataatc aagaattaaa tggagaatta
    28261 ctatttgaca caatacatcc aggagataca ttaaaaggtg agatagcatt tgaacttaca
    28321 aaagatacaa agccaaaaac aattgaaatt gcttatgaaa atgtaggaaa atatacagta
    28381 tttgatttat aataattatc ttaaaagaca cttattgaaa taagtgtctt ttattttagg
    28441 aggagtttaa tggaaatatg gttaagacaa gcaaataata catttaggtt tcctgttatt
    28501 ccaccctctt ttgagataaa tggaagtgca acaataaata catctaatat tttaagtgtt
    28561 ggagatattg cagtatttgg agggttaggg cttaaaacta tagaactatc ctcttttttc
    28621 cctaatcaag aatatagttt ctgtaattat aatggatttc caaaaccata tgattgtgta
    28681 aatttaatag aaagttggat gaaagaagga tatatattaa gatttatcat tacagaaact
    28741 aatataaact ttgaatgtat aattgcagat tttaattata gagaacaaga ttattcaaga
    28801 gatgtttatt ttaccttaag tctaaaagaa tataggagga tacaaatatc caaagtaaac
    28861 attaataatg atgaaaaatt atcttctgaa aaggatgttc cactaacaaa aggatttgat
    28921 actaagcaaa aaacacataa ggttgttgaa ggagataccc tttttaagat agcaaaaaaa
    28981 tattatggca atggagattt gtgggagaag atttataaag caaatgaaga taagattaaa
    29041 gatccatctg taataaaaaa tggttggata ttaataattc cttaaaatgg tggtgattta
    29101 taattaataa aatcaaaata caagttcata taaaaaacgg aaatatatat aatgtaactg
    29161 atttagtgga taagtgtact tggtctggtg attatagatc accatcaaga actttagaat
    29221 tttctataat tcaatctgca agtgatatta attttaggca gatagatatt ccagtagcaa
    29281 gtacagtttg tttctatgtt gatgataaag aactttttag aggaatgata atagatagat
    29341 ctaaagactc tagcaataat aatatagatt ttacagctaa agatatgggc tttttacttt
    29401 tacaaagcga agtgtcatac aattttaaag ataaattagt tgaagatatt gcaaagcagg
    29461 tttttgtaga taataaattc ccactaggta atttacctaa aactaacgtt aaatatacaa
    29521 aaatgtttat tggtgtaact ggctatgata ctataatgag cgtttataca gaagcaagca
    29581 agacaactaa aaaaaagtat atgatagagt ctaatcttga taaatttaat gttattgaga
    29641 agggtgtagt tactttaaat ataacatttg aagaaggctt taatcttatc aatacaaatt
    29701 tttctgaaag catggagaat gtaaaaaata aagtactagt tgttgatcag tatggtaata
    29761 aaattagtga gaaaataaat gactctatat ttaaagacgt tggggtaatt atgcaaaagg
    29821 taatacaaca acaagaaaac agtactatag acatagatgg agagtttaaa ggaatcgaaa
    29881 aaacttgctc tttaaaagga tatggtgata taacttgtgt aactggtaga ggtgtaaaag
    29941 taaaagactc atatacaaaa ttaataggtc ttttttatat agatacagat aagcatacat
    30001 ggcaaaatgg agattatcaa attgaactag agcttaattt tgagaatata atggatgaaa
    30061 aatctgctgg tcaagatgaa caaaaagaag atggttcaga gtatactgga ggaacagaat
    30121 actctgctga atttactgct tattgtccta gaaaatctga gggtggagat aaagattgta
    30181 gaggtaaaaa actagatcct tctaaaaaaa catgtgctgc tcctatggtt ggaacatatg
    30241 aaaaatctta ctatacaaaa gatttcttaa acaaacatcc tcttatgaga tatggagatg
    30301 aaatacaatt ggttacaggt gtttcaagtc gagatggaac gtataaggta aatgataatg
    30361 ggcctgcaat aattatagaa aaggatggaa catatcatat agatatttta tttggaaatg
    30421 ttgaagaagc caataacttt ggaagaagaa aagggaagat tataattggt ggttattctg
    30481 gaaacgtgac agaaaaagca aaaacagtaa tttcagaagc aagaaaacat cttggaaagc
    30541 catacaaatg gggtgggaat ggaccaagta gctttgattg ttctggacta atggtatatt
    30601 gctttaagaa ggttaatgtt aatttaccaa gaacctcaag tcagcaatct aaggtaggta
    30661 agaaagtaga gaaaaataat ttacaggcag gagatttggt atttttccat aatccagtca
    30721 gccatgttgg actatatgta ggtaatggag aatttttaca tgcgccacaa acaggagatg
    30781 tagtcaaaat aagtaaatta agtaatagaa aagattttaa tacagctaga agagttttgt
    30841 aaaagaggtg atatcgtggc agatccaatc aatgaattta taggaataat gagacaagaa
    30901 ggtaaatttc ataatgaacc ttcttttttt attggaaaaa ttaaaagtaa attaccagat
    30961 ttaaaaatag agataaataa catcatatta gaaaaagaag atatcttgat agacagttgg
    31021 atgcttgata gacagataga gttatttgat acagaaacaa gtcaagaaca taaacatgaa
    31081 ataaaaaatc cctttataga tacttttgaa tctggagaca cagtaataat gtttaaaata
    31141 ggtgaaaaat ttgctgttgt aagtaaattg gtgagtctag atgaataata ctatattccc
    31201 tttcatgggt gttccagaag attatatttc tccaaccaat gaagaattac ctatttttaa
    31261 agaatttgct tgggatttta ataaagatga aaagataatt gaaaatggag actttaaaat
    31321 agttgaaaga aatgaagcaa ttaaagtatg gatttacaaa acaataaaaa cagataagta
    31381 tgttcattta atatatgatt gggattatgg aacagatata aaaaatctaa tagggcaaaa
    31441 gtatactaaa ggtcttactg aaagtgaggc aaaaaggtat atacaagaaa ccctattaac
    31501 taatccatat atattagaag tcaatgtaac aaatacaaaa tttaaagatg ataatttatc
    31561 tataagttta aatgtaaaaa caatttatgg agaggaggag attacttttg cataatggtc
    31621 aatcatttag tgtcttaaat aatagaactt taaataacat aaatctacct ctttataaag
    31681 gtcaaggatc ttctctttat aatatagtct ctccaataaa ttcagaactt gcacagttat
    31741 atatagaact ttcttatata cataagagag tttttattca agataatttt gatgattttc
    31801 ttgatagaag agttaatgaa tttggagtat acagaaagtt aggcacagag gcaataggtg
    31861 aggtaatatt tgagggaaaa ataggaacta aaataccaaa tggaacaata atatcttata
    31921 gtgatttatt atttgtagtt attaaagata ttacgataga tgaagatagt aagttaaatg
    31981 taagtcctat acaggcttta gaggttggta ttaagtataa tttatcagct aagacagaat
    32041 ttaaacttat agaagaaata aatggagtaa ctaaaattta taatgaacta gacataaagg
    32101 gtggaacaga aatagaaact gatgaagaat taaaagagag gttctataaa atacaaaaaa
    32161 atcaagcaac aagcgggaat aaagcacatt atcagtcttg ggccttagag gttgaaggtg
    32221 tttataatgc aaaagtaatt cctcgctggg atggaccagg tacaattaaa atcctaattt
    32281 atggtcaaaa caatcaatca gttgacaatg aagtattaca aagatgcact gaacatatag
    32341 aagaggaaaa acctattggt cctactatta caattgttac accaagtact tttgatatta
    32401 ccattagtgc aactttaaca cttgaaaatg gatatgatat tgaatctata aaggttatgt
    32461 ttttggatat tataaattct tatttaatag aaaattcaaa agagattatt tatataaaaa
    32521 taatgagttt gttagcaagc atagaaggtg ttcacgatat aaaaaatctc cttgtaaatg
    32581 atgatgttaa aaacataata gttgatgaag aaaaagttcc tgctgtttca agtgctatat
    32641 ttgatatcga ggtgagttaa attgaagtta attgattacc tgccaagttt tgcaaataat
    32701 gaaattgaca tacaaatcca ggaagcactt gaaaatgaac ttctaacatt aattgacgaa
    32761 aaagatgatt tattagatca gttttttata gatacagcaa cttgggggct tgatgattgg
    32821 gaaagtctct tagatataaa aataaattat aagttggatt ttgatactag aagaagtaat
    32881 ataaaagcta aattaagagg caagggcaca actacaatag aagttataaa agctatatca
    32941 gaagcttata caaagactaa tgttgatgta aaagtgttca gtaatctatt tagttttaca
    33001 cttagttttt taacaaatga ttgtagttat aacactattt tagagttaga taagaaaata
    33061 gaggaaataa aaccttgtca tttagagcat aaatttgaaa tgattttatt caataaaaat
    33121 aacatgttta caggttctac aatgaataca ggtgaaacag ttactatata tccttacagt
    33181 acaactgata taacttcttt tggagaattg actataacta caggtaatga cagaagtatg
    33241 gaaaaagtaa cattatatcc tagacaggag gtggtatagg ttggcagaac aaaaatatta
    33301 tacacttttg acaacgatag gaaaagcatc tattgcaaat gcaacagctt taggcaataa
    33361 agtagattta gttaaattac agcttggcga cggagcaggg gcagaatata atccaacaga
    33421 agaacaaact tctctaaaaa acgtagtttg ggaaggttct ataaataatg taaagataga
    33481 cgaagaaaat ccaaactgga tagtaataga aactgtaatc ccgggtagtg tgggtggctt
    33541 tatgataaga gaagtaggga tatttgattc aaaagaacaa ctaattgcag tatctaaata
    33601 tccagaaacg tacaaaccaa cagctgacag cggaagtgta aaagacttag ttataaaaat
    33661 tattttagta gtctctaata cttcaagtgt aaatttgaaa gtagacccaa ctgttatttt
    33721 agcaacactt aaagacatac aagatttaga ctctaaaatt gatacaacta aaacagaatt
    33781 aacaagcaac atagaaactg ctaaaacaga gttgaataca aaaataggag atacaacaca
    33841 acttactaca acagataaaa caaatatagt taatgcatta aatgaggtaa aaactagtgt
    33901 agatagcata gaaacaacag cagagaaaac aagtataaaa gatatagata acttatttga
    33961 aagcgacaat gtagagggag cattaaatca attagcaata aattataata tattattgga
    34021 aaaacaggat aatttagaaa cagaagtgaa tggacagaga ataaaaggaa tttcaatagc
    34081 aaatagtttg atagacatga tataagagag gtgagaatat gacagaaaag ttgactgata
    34141 atgctagttt aagagaactt atgatagcat ttgaaagtgc aaaaaatgat ttgcagatag
    34201 ataaaaataa tatagccagt acattgggaa gtccattttt aggaacagat aaattagata
    34261 caactaaaac aaaaacacaa acattaaaaa atacacttgc aagtaatata actaataaag
    34321 gtattgtaac aaacagtaca gaaagtttgc aagatatgat aagtaaagtt agcagtataa
    34381 aagatttaag acaagcaagt ggtacagcta gtgcatttaa aggcagattt tatgatgatt
    34441 attactacct atggagtgta aatttaccac aattaaattt tgtgccactt gttgtttttg
    34501 tctatgctga ctatagacgg ctttcgattt gggttaaaag tcctttttct aaatatattt
    34561 tttatggtaa aaaagataat gtaaaaacag agttaaaaga tttttatata aaagagttag
    34621 acgatggaat ttctacagag acagttatac atctctatgg ggcttcagga gacacttatg
    34681 aagtacaatg gttagcaata ggtcaggagg cttaaatatg gaagtaggaa aattaataat
    34741 ttatgataat aaagggaaaa ttttatttca aggtggagaa ggaaaaggag atttaattcc
    34801 atatgacaaa gacatgaaag ttgattgggt agagataaaa tacaattcta tagattattc
    34861 taaatacagg attattggaa taaatgtaga aacaaaagaa cctgttttag aagaaataaa
    34921 agtagaatta acagaagaag aaaagagaat acaagaatta gaaaatcaaa tattgattgc
    34981 agaaaacgaa aaagtaggag gaattttata atgaatataa ataatgttgt cgtaagaata
    35041 ttggcagaga gaattttaag tggaggttta aaccctctaa aaaatcgaga atttgaactg
    35101 gatgatgtaa ctaatgcaga gtacagaaaa gcagtagagg attatataat tagagaaagt
    35161 ggagtaatag aaggaacaga actaacagta taggaatgtt ccttttttat ttcaattaat
    35221 taggaggctt acatgaatga agaacttttc gaagcagatt taaaaagaca tgaaacaaga
    35281 ataaataagc atggagaaga aatagacgaa ttaaaaatag caaatataga gtctaaagca
    35341 gagttgaaag cattgtgtga gaatctaaac tcacttacaa gtatgctcaa atggctaatt
    35401 ggtacaatga ttacaacatt agtaggattc tttatatttg cagttcaaag aggaatattt
    35461 taattaggag gataaaagat ggataattta ataagtttta taccagagca gttgctaatt
    35521 ttagtagctg ctctttatgt tataggagca ggttgcaaga agtataaaca actagataat
    35581 aagtatattc cagtagtatt actagcactt ggaataggtt tctcaatatg gatgttagga
    35641 ttaaatcctg ttgcagtctt acaaggcgtg atttgttggg gtatatcaat aggtataaat
    35701 caaacttaca aacaactaaa ggaggaaaat aaataatgaa aatagcaata gtaccagggc
    35761 atacattaag tggaaaagga acaggaacaa ccgggtatat agatgaagga aaagaaaaca
    35821 gaatactaac tgatttaata gtaaaatggt tgaaacaagg tggaactact gtgtatactg
    35881 gaaaagtaga taaatctaat aactatttag cagagcaatg tcaaatagcc aataaacaag
    35941 atatagactt agcagtacaa atccatttca acgcaaataa aacaacacta aacgcaatgg
    36001 gtacagagac aatatacaaa actaacaatg gtaaggtata tgctgaaaga gttaacaaaa
    36061 aactatcaac aatatttaaa aataggggtg caaaatcgga tgcaagaggt ctttactggc
    36121 ttaagcatac aaaagctcca gcgatattga ttgaggtgtg cttcgtagat agtaaagcag
    36181 atacagacta ttatatcaga cataaagaca tagttgctaa gttaatagca gagggtatat
    36241 taaataaaaa tataaataac aaagagaatg gtgagggaaa aatcatgtac aaacatacaa
    36301 tcgtttatga tggagaagtt gacaaaatac ctgcaactgt agttggctgg ggctataatg
    36361 atgggaaaat actgatatgt gatataaaag attatgtacc aggtcagacg caaaatcttt
    36421 atgtcattgg tggtgcagca tgtgagaaga ttggttctat gactaaagaa aaatttacta
    36481 tgataaaggg taatgataga tttgatacac tttacaaagc attagatttt atcaaataat
    36541 aacttgttgt aatatccaat caatataaaa tttattcaac ttaggaggac ttgcaacatg
    36601 cttaacatag aaaaatataa actatacaat ggaaactgtt tagaaataat ggatctaata
    36661 gaagataaat caatagattt aattctatgt gatcttcctt atggcacaac taattgtaaa
    36721 tgggacacaa taataccttt caaatcaatc tggaatcaat ataatagaat tataaaagat
    36781 aatggagcta tagtactatt ttcagcacaa ccatttacta ctagtttgat aaattcaaat
    36841 attaaaaact ataaatatag ttggtactgg attaaaaata aatctacagg attcgcattt
    36901 tctaaatacc agccacttcg aaaagtagaa gatattaatg tattctataa aaagctcctt
    36961 tatacaatcc tcaaaactta gaaaaattag ataaacctat tacatgtaaa aagaaaaata
    37021 aaaataaaga tggaatatat agacatcata ctttatcaaa agaatatgtt caagaatata
    37081 caaattatcc taataataca ttgtatttta ataaagaaac aaattgcatc catccagctc
    37141 aaaagccagt agatctttta gaatatctta taaaaactta tacaaacgaa aatgaacttg
    37201 ttttggataa ttgttttggt tctggcagtg taggaatagc ttgtgcaaat ataaatagaa
    37261 agtttgttgg aatagaatta gatagcgatt attttcttca aggtaaaaat agaattgaaa
    37321 gagcatacag aaacaatgca attgacaaaa acatagtaaa atcctaatat tagagaaagc
    37381 aaaggataat agataaaaaa actatcctcc tttgctttct ttatttttct atttctttta
    37441 ataatcttat acgttgcaat tacgcatctg ttaaatctaa gattaaataa ataaaaagac
    37501 tagaaaattt tattatcttc taattctttt tatttaactt atcacaataa aaataaatca
    37561 ttttttaatg acatttatat gatatttatc atatttagtt ttgaaacatt ctagcgttgc
    37621 tttttttctg gaataactta cctgtactat cttaaataaa aaaatgctac tatattataa
    37681 accaaatata aaataaatga tttagagcca gattatttag atagatattt tatacgatta
    37741 atgtccactg tgcataaatt taaaaatcta gatgaagaag attaaatatc tcccttcatc
    37801 tgctcttcac atattttaat agactttcaa gcaataattg acttagcaaa tcatgtttat
    37861 cataatgttt attctcatct gcaaatttat taaacatttc ccacactgta ttatttaccc
    37921 taattgtagt tctttttata gactgatctg gaagatctat agatatatca ttttctttaa
    37981 tgctatctat atgacattta tcatctttag ttttaaacca ttctatcaca ctttttatat
    38041 tatcaatttc attagataaa tatttgatat catcatcaat cttactgtta atgctatcta
    38101 tatgatattt atcatcataa cttttattat cttctaaaaa cctgtttcca actcttttat
    38161 atcctttctt attaagccac ttccttatag tactttcatt tgcccctaat tcatctccaa
    38221 cttttttaag actttcaaat tcacttaatt tattattaat acaatcaaca acttcattaa
    38281 tatttttttc tttcaaatca ctccaagtca tataaaacct cctcaaaaaa ataatagatt
    38341 acaaatatca tatatttgta atctattata catactataa actaaatcct tttttatttc
    38401 atcatttttt tgtatccttt tgcatttgca aaaacatgtt tatctacatc actcatgact
    38461 attatgccag gtatattctt tttaaatgct tcacctaaca atttagcacc tccacccata
    38521 acgacaacag cagaagttga tattgggtaa tttaatttca attcattata tatttgttta
    38581 aataatccaa cagacgcatc tatatttttc tttatatctt gtttttcacc atttaggtaa
    38641 aaccctttgt ctatgtagtc ttgtattttt tctcttttta tctcaacttt acccagaaat
    38701 ctttcatcca tttcaagaga tttttttatt gcatcatata tatttatcgt tccaacattt
    38761 acagaatatg gatcagtcgc tttattccct tgtacaagcg caatatctgt tgtctttcca
    38821 ccaatatcaa ctaaaacaac atctcttcct gcaatttggt caactatgtc ttttgcataa
    38881 tataagtatc ctgtaactcc ctctggaaac acttgaacag atcttattgt tatatctctt
    38941 ttagtagttt catttccttt tgcaaattca acatgatatt cttttccttg taatttttca
    39001 atcaaatctt ttcttaatcc tccaaattgc ttcactggta gacctaatgc tagatcaata
    39061 acttcatcat ctgtatttct gcatatagcc ccaagtaaaa gtggtaagaa gttttctttc
    39121 tcatatttaa ctatatttat ttcctcgtca ccttcgccta aataatatgt tttattacct
    39181 attttaatgc tatctgaatc tgaaccaaag ttttttacct ctgtaacttt actttcaaaa
    39241 atatcatctg tacttgtttt aactgcataa tttccaatat ctatacctaa tttactcatt
    39301 tatgttattt cctcctttta ttctaaactt ccaaaatcaa aattgttagc tggatcttct
    39361 atttcttttt tctgttcaac tacagcatta tccttttttt cattcatttt ttcccaaacg
    39421 agttctttta aataagctgt agctgataat ttctcatcta aaaacttttc aattgctttt
    39481 tctttatcat tgtttttaaa tgttagaact atcctactca ttcaaccact tccttttttg
    39541 taaaatatta taaaatttat taatatttac atatatttta ccaaatttca catagtaatt
    39601 caatagattc ataagatatt ttttataata ttttataatt ttttacgtaa ttttataatt
    39661 ttttataaaa tttatcattt tgtatataat taagaacaca ctttttataa ttttgcataa
    39721 aaaaaagagt gtttcatata tttctaaaca ctcttttcta ctttttataa ttatctaatt
    39781 gtaaaagaag cttcagctgg actagagaat aatataatac ctcctgtaca aacacctttt
    39841 cctttaaatt tagcaatata tgaacccttt tttagccctt tgaaacttac tacaccagtt
    39901 ttagtagttc cagctaatct aaaacttaca aattgagatg atacaatttt gccagcctta
    39961 tatacatcta tatttccttc aaatcctaaa atatttccaa gcgtagatgt tactctccaa
    40021 tgtaattgtc ctccactcat tgctgttaaa ttagcagtac cacctggaac aacaacagat
    40081 ttagtcgaag gcgaatctga gtttggttgg ccaattaatt caactgtttg tgaatttcca
    40141 gtaccttcat aatcatttcc atcagcaaaa gctgtaaatg gaatcattac tgcacaaagc
    40201 gttagtaata aagcaattgt ttttttcatt tgttttcccc ctattttttt atattattct
    40261 caattacagt ttcctttctt tacgtgcctt aataatcata ttgtaattat gctcagcagt
    40321 ttcaaatgct cctaccaaag gagttataat tacactttct attaagcaca attcatcata
    40381 gtttaaacta gcttctccag catagaaatt atttaactta tctaaatcta ttctagttaa
    40441 aacggaaact gattccattg agaatttgta aacatcaata agtttatata atccacctat
    40501 atagtttttt gctacttcat ggttcttaaa aaattcatct tttcctgtat gtaggtttac
    40561 ttccataatt ccctccattg ttcttatatt tttaaccttc aaataatatt tacttcttct
    40621 tcaaaacaga atattctatc acggtagaat attctgtttt tttcattcta tcacgataga
    40681 ataatatttt caatcatttt ttctattcta ttttatttaa atagaataga aaaaaagaac
    40741 acttaatatt tttcaagcta atatattaag tgttcttttt atatttttac aagattatat
    40801 aaaattattc tttaatcaat taaagtgttt ttctatttct taataataag aaatgatata
    40861 aaattgtatc attttaaaga ttttgtgata taataaaagc aagaagaact acaatctatt
    40921 taggcgtaga gtggagttca taataaacgt agtttatttt ttgaatttaa atataaattt
    40981 aaattcaacc tgtaagtcac tctttgcacg agagtggctt tttgcttttt tgaatagttc
    41041 actgattaag taaactacca cactagctgt taaactagct aaaacattaa acaaaaagtt
    41101 atccatgtaa ctcacctccc ctcgaatcgt tgggaggata atcttttgta catgaactcc
    41161 actctataga ttgtatatta cgctcttctt gctaaattta attataccat attttgtaat
    41221 atcagttaag agtttgcact acaataatca gaaagcttgt tccactcaca atcaacccct
    41281 attattttaa gaatatcatt gacttggcta tttaaatctt taatatctga tttagacatt
    41341 aaaggatctt tcattatttc atttagaaaa atactatcaa tgattaaaca tacattttct
    41401 gataaatatt cacgttttac tgagattact gttccaactg tactttcttt tagtgtctta
    41461 tatgctacac tcaatatgca tggttctaaa ttaattcctt ctagtactgt ttcaaagtaa
    41521 tattttattc ttatattttc atttccaata aatatttctt cattgctgct tatcttcttt
    41581 aattttctca ttatgcctaa catcttagca ttcacttcat aatttattcc atatctcttt
    41641 gatgccaaac tcgacattaa gtctctttct aaataaatta cccactgacc attataggaa
    41701 aatgatgttt tagcgatatc catttctcct tttccagtaa tattaaattt aggctctagt
    41761 ttatttatta agtaaatctc ataaaaatcc atagtatatt tattatctaa ttccatatac
    41821 tcaagtctta aattcttatt acaccaattt tcttttttat tacttaaatg actagcgtgt
    41881 ctatcacaga tattctctgt tttcccaacg tataaacatt cttccgtatc ttgattaata
    41941 tatttataaa cataatacat tttttatttt ctccaattct taattgtcat atttacagcc
    42001 cttatatatt tcaaataatt cttgccatgt atatccaact tctagtatgt gaaaaatatc
    42061 ctctatttgt tggaaaatct cagtaatttt atattcaaat gttggcatta attccattaa
    42121 attggccaaa gatccaatcc taatctctaa tgttatattt ataagtattt ttttatttgt
    42181 tgctgtatat tctttccatc tgggataaac tgaaatcaaa gaacaacttc caaatctttc
    42241 taatgtttca aaatcatatg atatcaaatt aatatctaat tcattctcac aaaattctat
    42301 atccatattt tcaaagttat atagcacact taaaacatta ttttcatata acatttcaac
    42361 cttggcatct aacttttcaa ttctctttaa aaaattaata gattttttac ttacttcata
    42421 tttcatatct atctttcttt ctctgaatct tctttttccc tttgtctttt ccttaaaatc
    42481 ttcttctgaa tatgtactcc aatcttcttc cttgtaatca aaaaagatgt gttcagtatc
    42541 catatcacct ttacttagtt tattaaatat tggatttagt tcatttatta aataaacttc
    42601 aaaaaaatcc atattatatc tacgtggaag ttcaatatat tttaatatta attctttagt
    42661 acaccaatct tcttctttat tactcaaatg agatgaatgc cttgcatata tatcttctgt
    42721 ttttccaacg tataaacatt cttctgtagc tggatcaata tatttataaa cataatacat
    42781 tttttatttt ctccaatttt tatttatagt aatatccttc ttcatttact agaccttgat
    42841 caaataaatt tctcctaaca gtttcaagtc tgacaggatc tttgctataa tcaacttctc
    42901 tttcttcttt tttaacatct tcatcatccc aatatttatc taattcttct ttaaacttta
    42961 aatcatcttt ttgatgaatt ataacttcac ttattttatt tgaaagtgtt gatttaaaaa
    43021 aatcataatt ctttgtattt ataatatcag tattatctct ttcaaatgct atagcaatag
    43081 acttattaaa agcattcaga taaacatctt tcttaaaatc aaactcttta aaatcttctt
    43141 taaatagttt aatcgtccct gttgtaaaaa tattttcatt tggtatataa aaactaaaag
    43201 acacttcatt gtcctgcttc tttttaggta tataatcttc aaccaatata tctttatcaa
    43261 aatattttct aggttcataa tctatcacag aaaaaattat ttcatctatt tttctatttt
    43321 ttcttttttc ttcttttatt gaaacaacca tgttgccttt tttatttatt tcattcattg
    43381 ctctttttag aacattttgt ttaaaatatt tatactctgg atatacatta tcttttaatt
    43441 tcaaataaaa tcttaattca tctaacttat attttatctc cacttcctta ttttctctgc
    43501 tccatagtct aaataaagtg tataacctct gagtatatgc acctctaaaa ttaaataaca
    43561 ctgataaatt aatagctgta tatccatttt tctttttctg catttttaca aaatcagtta
    43621 tatgattgta tagaacttca tgcatcataa ttgtataaat ttggtctgta tgatccaatt
    43681 catatgtatt aattaaacca cttccaaaag tttttaactt gccagttgtc tcttctatat
    43741 agtcaaattc caaaacactt tgttgaaaca tgttcaaaat ttcttttata tttttatgct
    43801 cataatcatt attgtttttc ataaatactt ttagttcttc ttttgatata gttgtaacat
    43861 aagatgaatt ttgctgtttc tgagcattaa aaagtatttt gtaaaataat ttattttcca
    43921 catttgtaaa atcatattta ctttttatta aattatttgg ttgcattaaa atttcttttt
    43981 tttccaaaat ctatcacctc atataaatta taatacctaa tttataaaaa ggtcaactat
    44041 ttttgaaaga cgctcacctt tgacccttaa aatgatgacc tttgaccctt aaaatgatga
    44101 cctttgaccc ttaaaatgat gacctttcga cccttaaaat gatgaccttt gacccttaaa
    44161 atgatgacct ttgaccctta aaatgatgac ctttcatctc tgaaacctag atataccaat
    44221 tattatagct tgtctaaaaa ctattaaaga ctttaaaaac tgttttaaaa actattaaag
    44281 actagaaaag atgttctgaa ctattctttt tttatattaa aaaactttag tatttatttt
    44341 tctagtatac tttaaacact tactaaaaat aatttcttat aaaagattgg agatgaagaa
    44401 ttttttatgc ttgaaaacag aaaacaaatt cagaaagaaa tttacttacc aatagcaaca
    44461 tattgtttaa aagataagca atattatata aaagaatatg gagcactatt aataaagagt
    44521 gctgaaaata gtgacatttt taatacccat aaaaatagaa ttttgtataa gaataaactt
    44581 gatattcaag aaatagcaaa tgaactgaat gtaaataaag caacaatcca aagaaatata
    44641 aaaaaactag agaagttaga ttttaaaatt ctaaaaatag aaaataccct aaatggaatt
    44701 gtatattgtt tgccgtcaga aaataatatt gatttaaaca aatttgcatt gataaattat
    44761 gaaatgctta aaaaaatggt caataaattt aaatcaaata caataaaagt atatttttta
    44821 cttaaaacaa tcactacaga aactaatttt aaacctgcaa ctaatagttt tatagcagaa
    44881 aatataggac taagttctaa aagtaaaaat aatttagata tcataacttc ttctgttaaa
    44941 actttagaag aaaataaata cattgaaaca aaaaaagtaa atgtatatga atatgacaaa
    45001 gaaaaactta gagaagttcc aaaaataaga aaagtttatc ggatttgtaa ttttgatgag
    45061 tggaaaaagg aaattgataa aaataaagtt tattaaatat agcttaaaag catttaaagg
    45121 cttgtctgac gtgttttaat tacaaacttg atgttattta cctaaaaaga tacaaatttg
    45181 cttaaaaatg gaaatagaag gtcaaatata tagatagaga accaaataaa attatattgg
    45241 ctctctattt atcttcttcc caatcctcaa taaacttttc taaacttttt aatttatttt
    45301 cagacacttc ttcatcatca tgcagagcac ttataaaact ttcaaaagaa tttttgtgaa
    45361 agaaactaaa gaaactttta gtctcaactt ttaaatattc ttctttttct attaatgctt
    45421 tataatatgt aaatctatct ttcttaacaa cttctaaaaa tcctctacca actaaccttc
    45481 ttaaaaaagt taacatagtt gattttttcc attcatattt ttcacctaat gtttttaaaa
    45541 tttcagtaga tgcaacttct tcatcttcac tatcccaaat gtacatcatt accaaaagtt
    45601 ctgattctgg aatctttttt atcttcataa agcacaccac cttttcaaaa gtataactaa
    45661 aatactataa caatagagtt aactatacaa tactaaatgt ctaaaaatgt tattccaatt
    45721 tttaccaatt aatttttctt taaaaaaaat ttaagcagga gtaaaaaaat aaaaaattat
    45781 caaaatttat caatataaaa agaaatataa gtataaattt ttctctttgt atattgattg
    45841 tatacaattc tatttttgta aaattaaatt ttacaaaata atcaaaaatg gtaaattttg
    45901 acatttgcct aatgtcaaat taggtgttac aattgtaaca taaagaagat atatatcgaa
    45961 ataatacata taaaatacat ataaaactac gtacgtaaaa tatttttaaa aataatcaaa
    46021 aatcttaatt ttcaatattg aatacaatta aattttcaaa ctcatctagg gcatgacata
    46081 caaaagcaaa aaacattaat ttagtatctg ataaaaattt aaaaattata aaagggggaa
    46141 gtctgttatt tttcagaagc aatgtaactg taaaaatata aatcagaata ttttgaaaat
    46201 atgggggatt ctatgggaga attaaaactt gaatcaaaaa tgaattgcac taaaaataag
    46261 aaaaaagttg ctatatctat acatcaaatg aaaaaaattt ctaaaaaaga ttatgttttt
    46321 taacagattg catagaaaca ttttatatga aaataaaaga ggtttgataa attttcaaac
    46381 ctctttttat tgctatttgt ttttcttttg caataattct atcattttca agatagcatc
    46441 tctatcttct tgttctaatt cataaagttt gttagttatt tcttttacat catcatcaat
    46501 ttctaagtca gctaaacaat ctttataaat ttcacctttt ccacttctta gccattcctc
    46561 atttacttca aattctctgc atatgtcgtt aatacttctt tctgtcaaaa ctctttttcc
    46621 tttttccaat aaagaaattt gatcttgtga taagaaaatt ctttccccaa acgctctctg
    46681 cgaaagtctt tcaagagttc ttaactcatt taatcttttt ccaatttctt tagcttttaa
    46741 tttttttgaa acttctgcca tttttacaat cctcctctgt cattactata ctataaaaac
    46801 acgactttgt cattaaaaaa ataattataa cgacaaaatc atgataaaaa taaaaaagat
    46861 attgattttt aacgacaaag tagttatact taaaataagt tgaacgacaa agtaagtgta
    46921 ggaggataaa aaatgatttg ttcaaacaaa gagaaagtag aaacagcttt gatgatagat
    46981 tattt
//
LOCUS       DAMJYC010000025        46130 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJYC010000025 DAMJYC010000000
VERSION     DAMJYC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.026"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            164..3712
                     /locus_tag="PC472_001506"
     CDS             164..3712
                     /locus_tag="PC472_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2726836.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYENGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSADIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPITHVGDLCYKAIWNNQDKLCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3897..5831
                     /locus_tag="PC472_001507"
     CDS             3897..5831
                     /locus_tag="PC472_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2726837.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMEDKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGINKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5937..6932
                     /locus_tag="PC472_001508"
     CDS             5937..6932
                     /locus_tag="PC472_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2726838.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6904..7689
                     /locus_tag="PC472_001509"
     CDS             6904..7689
                     /locus_tag="PC472_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2726839.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7677..8375
                     /locus_tag="PC472_001510"
     CDS             7677..8375
                     /locus_tag="PC472_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726840.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLKASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8498..8971
                     /gene="ybaK"
                     /locus_tag="PC472_001511"
     CDS             8498..8971
                     /gene="ybaK"
                     /locus_tag="PC472_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2726841.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9168..10007
                     /locus_tag="PC472_001512"
     CDS             9168..10007
                     /locus_tag="PC472_001512"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2726842.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10020..10568
                     /locus_tag="PC472_001513"
     CDS             10020..10568
                     /locus_tag="PC472_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2726843.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10634..11815
                     /locus_tag="PC472_001514"
     CDS             10634..11815
                     /locus_tag="PC472_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2726844.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQREMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12110..12496
                     /locus_tag="PC472_001515"
     CDS             12110..12496
                     /locus_tag="PC472_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2726845.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYNMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12646..12909
                     /locus_tag="PC472_001516"
     CDS             12646..12909
                     /locus_tag="PC472_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2726846.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13038..13880)
                     /locus_tag="PC472_001517"
     CDS             complement(13038..13880)
                     /locus_tag="PC472_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2726847.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14175..14909
                     /locus_tag="PC472_001518"
     CDS             14175..14909
                     /locus_tag="PC472_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2726848.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14919..16052
                     /gene="nagA"
                     /locus_tag="PC472_001519"
     CDS             14919..16052
                     /gene="nagA"
                     /locus_tag="PC472_001519"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2726849.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16151..16900
                     /gene="nagB"
                     /locus_tag="PC472_001520"
     CDS             16151..16900
                     /gene="nagB"
                     /locus_tag="PC472_001520"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2726850.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17075..17347
                     /locus_tag="PC472_001521"
     CDS             17075..17347
                     /locus_tag="PC472_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726851.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18007..18711
                     /locus_tag="PC472_001522"
     CDS             18007..18711
                     /locus_tag="PC472_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726852.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18713..20200
                     /locus_tag="PC472_001523"
     CDS             18713..20200
                     /locus_tag="PC472_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2726853.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIEFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20405..22018
                     /locus_tag="PC472_001524"
     CDS             20405..22018
                     /locus_tag="PC472_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2726854.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22380..23210)
                     /locus_tag="PC472_001525"
     CDS             complement(22380..23210)
                     /locus_tag="PC472_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2726855.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23283..25010
                     /locus_tag="PC472_001526"
     CDS             23283..25010
                     /locus_tag="PC472_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726856.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSVSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25047..26909
                     /locus_tag="PC472_001527"
     CDS             25047..26909
                     /locus_tag="PC472_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726857.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27242..27931
                     /locus_tag="PC472_001528"
     CDS             27242..27931
                     /locus_tag="PC472_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2726858.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28036..28722
                     /locus_tag="PC472_001529"
     CDS             28036..28722
                     /locus_tag="PC472_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2726859.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28739..30646
                     /locus_tag="PC472_001530"
     CDS             28739..30646
                     /locus_tag="PC472_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2726860.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31099..31788
                     /locus_tag="PC472_001531"
     CDS             31099..31788
                     /locus_tag="PC472_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726861.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32224..32763
                     /locus_tag="PC472_001532"
     CDS             32224..32763
                     /locus_tag="PC472_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726862.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32782..33825
                     /locus_tag="PC472_001533"
     CDS             32782..33825
                     /locus_tag="PC472_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726863.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33827..34663
                     /locus_tag="PC472_001534"
     CDS             33827..34663
                     /locus_tag="PC472_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726864.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34768..35442
                     /locus_tag="PC472_001535"
     CDS             34768..35442
                     /locus_tag="PC472_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726865.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35442..36494
                     /locus_tag="PC472_001536"
     CDS             35442..36494
                     /locus_tag="PC472_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2726866.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36724..37572
                     /locus_tag="PC472_001537"
     CDS             36724..37572
                     /locus_tag="PC472_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2726867.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37562..39226
                     /locus_tag="PC472_001538"
     CDS             37562..39226
                     /locus_tag="PC472_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2726868.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39275..40531
                     /locus_tag="PC472_001539"
     CDS             39275..40531
                     /locus_tag="PC472_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2726869.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40554..42635
                     /locus_tag="PC472_001540"
     CDS             40554..42635
                     /locus_tag="PC472_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2726870.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            42672..43757
                     /locus_tag="PC472_001541"
     CDS             42672..43757
                     /locus_tag="PC472_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2726871.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            43811..44935
                     /gene="wecB"
                     /locus_tag="PC472_001542"
     CDS             43811..44935
                     /gene="wecB"
                     /locus_tag="PC472_001542"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2726872.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENINSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKHSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            45013..45987
                     /locus_tag="PC472_001543"
     CDS             45013..45987
                     /locus_tag="PC472_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2726873.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18398 a         4679 c         7995 g        15058 t
ORIGIN      
        1 aataaagatt ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg
       61 taaatgtata ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt
      121 ttgcaaaaga agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa
      181 gccaagaatt ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc
      241 tgttttcaag tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt
      301 gtctgagttt acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga
      361 catgcttgaa tctattgcta acttcatagg aaacttgaat aatataaata atgaaatggt
      421 gattgattta atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac
      481 attagatggg gaatgttatt ctaatgatgg aatcacccat aatgttcaag atagagatta
      541 ttttaaaaaa gctaaaaatg gagaaaagaa tatttcagaa cctttaaaat ctattgttaa
      601 taataaagaa ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt
      661 tttaagtgga atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga
      721 tgaaaaagca gatgtttttg ttgcaaagaa aaatggagat atagttgccc aaaaaacaaa
      781 taaaaataga aagacgctat catgtatatt tcagcttttt gacaaagata attgtgatga
      841 agatgattgt ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa
      901 ttataactta gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg
      961 gtatgttttt tctgttgtgc caaataaaat agtatctatt cagtcagaga atatatctta
     1021 tgatgtatat atttttgcat tagaaatact actagttata ttgattttta taatatatat
     1081 agcatgtatt ataaataata acactaaaat tattataaat gaaaaagaaa aatttaaagt
     1141 actcactgat aatatactag gaggggttaa aacctgttta aaagatgaat atatgactat
     1201 gacatatgta agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa
     1261 aatatttaaa aatagattta aagatatgat ttataaagat gatttagaca agtttaattg
     1321 tattattcta aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag
     1381 aaaagatgga agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg
     1441 aagtgagtgt attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga
     1501 attagattca aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt
     1561 gataactgag ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat
     1621 gattggatat acacgcaaac aactgaagga tgagttaaat aataaattaa tcaaacttat
     1681 ttttaaagat gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga
     1741 tacatttgca tctgaacaaa gagttgaaaa aagggatggc actattatat ggattttatt
     1801 aaaaggaaga aagatgattt atgagaatgg aaaatctgta ctttgttgtg ttattgtaga
     1861 tattacttct gctaaattat tagaacaaga acttaaaaag agtgaagaac gtcatgaaat
     1921 tattctaaag cagacgcaaa atattataat agaatgggat atagaaaaag ataaaatgaa
     1981 tatatcacct aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa
     2041 aaatataatt aaatcagcag atatatatca tgatgatatg gataagttta tttcattaat
     2101 taaacttact tcacaaggga gtgaatatga agagtgtgaa attagactta gaactagaga
     2161 tactatttac gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc
     2221 ttataaagta gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt
     2281 gaaagaaagg gcagaaaaag accctcttac agatttgtat aataaaaaag catcacaatc
     2341 acttatagaa gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga
     2401 tattgatgac tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt
     2461 aagtgaaatt gcaaatgacc taataaatct atttagagca accgatatag taggaagaat
     2521 tggtggagat gagtttattg tatttataaa agatatttct gaaacaagag atattataaa
     2581 taaagcagaa gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta
     2641 taaaatatca ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac
     2701 actatttaga aatgcggata tggctcttta ctcagccaaa gggaagggta agaattgtta
     2761 tacattgtat gataaaaaaa ttgataaagt ccaatatata actaatacaa gaaatgcagg
     2821 agatagtgat aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat
     2881 actatatgaa tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa
     2941 acactttaat gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa
     3001 aaatacattt gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat
     3061 ttgttacaag gatttaggca actatctttt aaattttaat gcagatgggt tattttattg
     3121 taatgatatt gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg
     3181 ttcaatatta caatgtaaaa ttttagaggg taatgaattt agagggttca taggatttga
     3241 tgaatgttca tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc
     3301 taaaacgctc agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt
     3361 aacaattcaa aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa
     3421 tacctatgaa ttactattta tcaataaaaa aatgcttgag tcagcaccaa tcactcatgt
     3481 aggtgattta tgttataaag cgatatggaa taatcaagat aaactttgta aaaaatgccc
     3541 tatgatatat ttaaaagatg gtgatgatac ttataaatca ttcatgcata ataaaaagtt
     3601 tgaggtacag gcaaatataa ctacaactac tttgaaatgg tttgatggta aagatgcatg
     3661 tttaatattt ggaagtgaaa taactagtat tgaagctgaa gaaaatactt aaaatactgt
     3721 gttatatata tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat
     3781 catttaagtt gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta
     3841 aaagatactt ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg
     3901 aaaataagtt tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag
     3961 attttgttct agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa
     4021 tatctagagt tttagaaagt gctgggtata aagttggaat aatagctcaa ccagactgga
     4081 gaactacaga tgattttatg aagctaggaa aacctagact tgcatttcta gttaatgcag
     4141 gaaatatgga ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata
     4201 tgtattctcc aggtgggaaa atgggttata gaccagatag agctactata gtatattgca
     4261 ataaaataag agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagct
     4321 taagaagatt tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag
     4381 atagtggagc agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca
     4441 atgcacttaa tgatggatat gacccaaaat acattagaca tattgatggg acatgctata
     4501 tttcagatac attggaagaa atatatgata aatatatatt aattccatca tataaagaaa
     4561 tttgtgaaga taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc
     4621 catttagagg taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc
     4681 cagaaaaacc acttagtaga gaagaactag atgaagtata tggattacca tatcagaaga
     4741 cataccatcc tgtgtatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta
     4801 gtatagtaag ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc
     4861 aaggtagagc agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa
     4921 caaatttaga tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta
     4981 gaagaccagc atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat
     5041 ctccatcacc atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag
     5101 ctgtaagaaa attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt
     5161 atgtaatgga agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg
     5221 gtcagttaaa ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac
     5281 cagcaggaaa aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag
     5341 gtaaaaagca atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata
     5401 gcgctataga gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg
     5461 atttttatcc aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc
     5521 ttacaatgaa gccagtttat gttcctaaat caaaacgtga caaggcaatg caaagggctt
     5581 tattacaata tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag
     5641 gaagagaaga cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc
     5701 cttattttaa tagaaataat tctaaaaaaa atgtatctaa aggaataaat aaaaacaaaa
     5761 aaactaatac aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga
     5821 aaaaacatta aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca
     5881 gaaattagta taaataaata taattatttt aatgacgtta cctttgggag gaagagatga
     5941 aaaagtatat ttctataata ttattagtgg tgttgactat ggttgttttg gtaggatgtt
     6001 caccaggaaa agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag
     6061 ctgttccaga tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga
     6121 ttaaagaagg atatgaaaca gtgtatagca ttgaaaagac acctgaggcg atttccacta
     6181 gagtgatgaa aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt
     6241 ataataaaac ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg
     6301 tgtcaacaga aaatataaaa gattactctg atttagttgg taaagaagtt ggatgtactg
     6361 gaaaaggact tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt
     6421 attcagatat aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta
     6481 ctggtaaagt taaaacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa
     6541 atccagacat aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa
     6601 aagatggtta tcctcaatct actttaattg taaagtctga atttttaaga gataataaag
     6661 attttgttga ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc
     6721 ctgaagaatt aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag
     6781 gaaaatctct tgaaagagct aatttaaaat atattccagt taaggacatg attaaagatt
     6841 ataaaaatta ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac
     6901 cagatgaagc aatatacttt gttgagaaat aaaaaaaata aagaacaatt tatagataaa
     6961 atggaggtaa cactttcttg tgttatcctc ttatttttat ggcaaattat tgctttaaaa
     7021 attgataatg atattttttt accaacagta ggtcaagtat ttaatagtat aaaagagatt
     7081 atgctaaatt ctagtttcta tatagatata ctttactcta tggggagatg tttttttagt
     7141 tttttactgg ctatgatttt tgctattata ctttcaatag tgtcttattt aaatagattt
     7201 tttagaaact tattaaagcc aatcaatgct ttgacacgtt caataccaac tatgatattg
     7261 gtagtacttg cacttatttg gtttgaaaag gatagtaccc cttttatagt tggatttact
     7321 atagtttttc ctatattgta tgataacgtt ttgggcgcta tattgaatat agacaagaac
     7381 ttattagaaa tggctaatgt gtataaaata aggtttatag ataaggtgtt aaaaatttat
     7441 ttaccagcca taaaatttca aattatatct atactagtat caacattctc tttaagtttg
     7501 aaagttgcta tagcaggaga ggtatatagt caacctacat atggtattgg aactatgata
     7561 cagactgaaa aaattaactt taacacatct ggaatatttg catggataat tattgttctt
     7621 ttaatatctg ctattttaca aatagctcaa aagtttttag caaggagagc ttttttatgg
     7681 aaaagataaa gttgagtatt gaaaatataa ataaaagata tgatagtcgt attatattta
     7741 gagactttaa tattgatttt tatgtaaatg aggtaaattg tatccttgga aaatctggtt
     7801 gtggaaaaac tacactcttg aacataataa gtgggattat taaaaatgac accaacaatc
     7861 taaatatcaa agaaaattta aatcgagtag gaaataagct taaagctagt tacatatttc
     7921 aagatgatag acttatagac tggttaacag tagaagaaaa tataaagata gttgtaaata
     7981 aatactataa taaaactcaa ttaaataaaa tttgtgatga atacttggaa ttggttggaa
     8041 ttagtgatta taaaaaattc tatcctcaga tgcttagtgg aggtattaga caaagagtaa
     8101 atattgctag agcttttata tatccatcaa aaaacattat aatggatgaa ccttttaaat
     8161 ctattgatgc aaagaataca cagcttataa tggataattt cagaaatata ttaagaaaag
     8221 aaaagagaac tgtgttattt gtaactcata atattgagga agcattgttt ttagcagata
     8281 ggatatttat tttaggagac tctccaatta gaataaaaaa aatattaaaa aattctaagg
     8341 aattggaaaa aaatgaagtt ttgaagttga tatagagtta taataatact gtcgttttaa
     8401 aatatatttg gttaaaatct ataagactat attaaaatag tacagaatat tcaaaactgt
     8461 gtataatgtt cgtattttat aaaaaggaag tgaatgtatg gttaagacta atgctatgag
     8521 aatattagat tcaaataaga ttgattataa agtaatgtct tatgaagtga aaagtgaaca
     8581 tgtggatggt gttgaggtgg cacatgatat aggaagagat gtaaatgaag tgtataagac
     8641 attagtaact caaggagtta gtaagaatat atatgtatat gtaataccag tacatgaaaa
     8701 cttagactta aaaaaggctg caaaagtagc taaagagaaa agtgtagaga tgattcatgt
     8761 aaaagatata aataagctta caggttatat tagaggcgga tgttctccta taggtatgaa
     8821 aaagctttat aaaacttttg taaatgaaag tgcaaagaat ttagatacaa ttattgtgag
     8881 tgcaggaaaa ataggatatc aaatagaact atctccattt gatttacaaa gacttataaa
     8941 agtagaattt gtagatgtaa taaaaaaata agaattacat ataaaatata gagcttatat
     9001 tcttaataat aggtgttgta gtattaataa aatgaataat aattttaaaa taaatattca
     9061 gttacttatg tgtaattggt ataaaaatat tgagaaattt taaaaacaat ggtaaagtgt
     9121 aagtataatc acgaatattt tttataaagt aatggaggga tgttcgcttg agaaaaataa
     9181 aatctgaaca aatcgtcgag caggtaaaaa aactttgtat agaagcgagc ctatatttag
     9241 gagaagatgt tttaagttgt ataaaagaaa aagcgaaaag cgaaaagagt gaagttggaa
     9301 aaaatatatt gaatatatta gttgaaaatg ctgaaatagc taaagaaaaa aatataccaa
     9361 tatgccaaga tactggtatg gctgtatttt ttgttgaaat aggacaagaa gttttaattg
     9421 aaggagacac acttactgat gctataaatg agggagtaag acaagggtat gaagaaggct
     9481 acttaagaaa atctgttgta agtccaatta acagagtaaa tacaaaagat aatacacctg
     9541 ctgttattca ttatgatatg gtaaaaggtg ataaaattaa aatagaattt gcagctaagg
     9601 gatttggaag cgaaaatatg agtaagatga aaatgttaaa gccatcagat ggattggaag
     9661 gaattaaaaa attcataata gatacagttt cagaagctgg acctaaccca tgtcctccta
     9721 tggtaattgg agttggtata ggtgggactg tggacaaatg tgctcaaata gctaaaaagg
     9781 cactttttag agaactaggt gaatttaata aggacgaaaa tatagcaaaa cttgaaagtg
     9841 aactattaac agctattaat aaacttggta taggacctca aggcttaggt ggtactacaa
     9901 cagctttggg attaaacata gagacttttc ctacacacat agcaggtcta cctgttgttg
     9961 taaatataaa ctgccatgca tcaagacata agaaggtagt aatttaggag gaaaataaaa
    10021 tgataaaaat aactacacct gtaaatgaaa ttgatatagc aaaattaaat tgtggagata
    10081 caattagttt gagtggtata ctttatacag caagagatgc tgctcataaa agattgatag
    10141 attgtataaa taaaggtgaa gaattaccat ttgatgtata tggtcaagga atatattatg
    10201 taggacctac tcctacaaag ccaggagaag ttataggtgc tgcaggtcca acgacgagtt
    10261 atagaatgga tgatttgact atacctcttt tagaaagagg tcttagattg atgataggca
    10321 agggaaaaag aagtgatgaa gttatagaag gtatgcaaaa atacggagca gtttatcttg
    10381 ctgcaatagg aggagctgga gcgtatattt caaattcaat aaaatcatgt gaaattattg
    10441 catatgaaga tttaggagct gaagctataa gaaagattgt agtagaagac cttaaactta
    10501 cagtagcaat agatagctat ggaaataata tatatgagca aggtcgagca atttatgaat
    10561 gtaaataatg tttagaaata aatatgaata tcaaaattaa aaatagttgt gtatatttta
    10621 aggaggaaaa ttgatgtcta aggattatgc aaaattggca ttagaaatgc atgaaatcaa
    10681 caaaggtaaa gtaagtgtag aaagcaaggt agaaattaaa actaaggatg atttatcaac
    10741 agcatataca ccaggggttg ctgaaccttg tttaaaaata catgaaaatc aagatgatgt
    10801 ttatagatac acttctaaag ggaatctagt agcagtagtt agtgatggaa gtgctgtact
    10861 tggattaggt aacattggtg ctgaagcatc tatacctgtt atggaaggta aagctatatt
    10921 atttaaacaa tttgcagatg tagacgcttt tccaatatgt ttaaagacta atgatgtaga
    10981 tgagatagtt aaatctgtag agttgatgga gcctgtattt ggaggtataa atttggaaga
    11041 catatcttct ccaagatgtt ttgagataga agagagatta aaaaaatctt taagtatacc
    11101 tgtttttcat gatgaccaac atgggacagc aataatagtt gcagcagcca taataaactc
    11161 aataaagtta attgaaaata aaaaaataga agatttagaa atagttataa acggagcagg
    11221 agctgctggt attgccatag caaagatact acttaatatg aatgttaaaa atataatttt
    11281 atgtgataga agtggagcat tggaagctag tatagaaaat ctaaactatg ttcaaagaga
    11341 aatgttaaaa gtaacaaata taagaaatga aaaaggacct ttaaaagata ttataaaagg
    11401 aaaagatgta tttattggcg tttcaggtcc aggagctgtt acaaaggaaa tggttgaaag
    11461 tatgtctgag aagccaataa tattggcaat ggcaaatcct actccagaga taatgccaga
    11521 agaagcaaaa ttaggtggag catttataat gggaacaggt cgttcagact tccctaatca
    11581 agtcaataat gtactggctt ttcctggaat atttagaggt gcacttgatg ttaaggctaa
    11641 agagataaat gaagaaatga agatagctgc tgcatatgca attgctaata caataagtga
    11701 tgaagaaata tgcccagagt atatattacc agatgtattt aataagaatg tagtaaaaaa
    11761 tgttgctaag gcagttaaag aagcagcgat aaaaaccaaa gtaaatagaa tataaatttt
    11821 tatatgttca tatatcctta tcttatcggg tatatgggca tttttattat aggtttatac
    11881 tcatatatcc ttacttttat atacatatct ttatgattaa aggtgttaga aaatgtagta
    11941 aattttatac tatcagactc tatctttatt actttagtag atacatggtg gtaaaggttt
    12001 atagaaaaaa atataggaag gcagattttt attgttgcgt atatatgaga atgatataat
    12061 atgaatatta taaattaata aatttaaatt tatggaggta caagagatga tggaatataa
    12121 agttaatgac caagaactta atgcttcaat gtttatccca ttcgtcaaca ggatatggcc
    12181 aggagattat aatatggata aaacacagac tgcattatca aaaactttga atataactgc
    12241 ttatgatgat aaagtgcttg taggatgtct gcgagttctt tcagatggtt attattttgg
    12301 aacaattaca gaattgcttg ttcttccaga gtatcagaaa caaggcattg gtagtaagct
    12361 tttccagctt gctaaagaga acaccccaac tatgctatat tttggtgcac agccaggagt
    12421 agaagagttc tatgaaaaaa atgggtgcca aagaagtctg caatcctaca taataaaaaa
    12481 gaaagatagt tattaaagtt ccagctttca ataatcttaa aagtacaata ttttagtgga
    12541 atcatttcat tttttattac taatatgaca taaacatgtc atattagcat tgatatactt
    12601 ttaatatggt taagagccaa agaaaaatat aaggagtgat ttaaaatgga agaaactaaa
    12661 ttttgccaat gctgtgcaat gccaatggga caaactgatg aattatatgg gacaaataaa
    12721 gatggaagta aaagtactga ttattgtagt tattgtttta aagatggtga gtttacagct
    12781 gatataagta tggaagagat gatagaagtt tgtatccctc atatgcttca aagtaataag
    12841 gatatgactg aagatgaagc aagaacaatg atgaacaatt tctttccaac attaaaacgc
    12901 tggaaataat tttatgcaaa attagttata tgcaaaacat gaaatatggt gttgagaata
    12961 aggggtggtg tgctttatag cataccacct gttttgttac ttggagttca gttattttcc
    13021 taaatatttt aaatatacta ttttagataa gttccctctt catcttgata aattaatcct
    13081 tttttcataa gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata
    13141 gaaatatctt tagggtcaga tttatcatta aatctcatat atccatcaga accttctaaa
    13201 taacttaaaa tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa
    13261 agaccaagtt ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca
    13321 gtttttagtt cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca
    13381 catatcattg cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct
    13441 acattgtaag tatggtctgt taataagtat gaatctatgt ctgtagtagc agcaattctt
    13501 ttgcttttgt ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc
    13561 ttagctttaa aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg
    13621 tctgtattat caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt
    13681 aaagttgcag cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct
    13741 atgtttattt cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta
    13801 ttagtttgtc catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta
    13861 aaatcaccta tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca
    13921 taatatactt atgtagtagt ttgtcacagt atctatatta gtattagtga aatttacaat
    13981 aaataatata gttatttatg tatttttcaa ttctctagct tcttatatag tataaactcc
    14041 aatttctttt ttgtctttag gttttattaa aatttttaca aaataagtta aaaagtattg
    14101 aaataaaatt tcttttaaag tataattggt atataccaaa attggtatat accatatcga
    14161 aaagtggtga ctatatgaaa atagtaaata aatctagtaa tataccattg catactcaat
    14221 taagttcaat aataagagag atgatagaaa ctggtgagct taaagagggc gatgcaataa
    14281 tgccagaaag agaactttgt aacatacaaa atgtaagtcg tatgacagta aataaaacca
    14341 ttgttggatt agttacagaa ggacttcttt atagggtaca aggtaaaggg acattcgttg
    14401 caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg ttttactgat gttatgaaag
    14461 aaaagggagt aaacataaaa acggatatat tatcctttga gatggaacta ccagatgatt
    14521 tagttaaaag aaagcttgga ataagtgata atactacaaa tatatataag attgtcagac
    14581 ttagatatac tgatggagag ccatttggat tagaaatagt atatctatca gaagaaatgt
    14641 gtaagggtct aacaaaaggg atattggata attcttcatt atatagggtt ttaaatgaaa
    14701 aatatggcta taagatacaa aaagctgaac aggtaatgga gccagtaata ctttcagatg
    14761 aagaaagtaa gcttcttgaa acagatgaag gtgctttagc tcttaaactt catagaaatt
    14821 cctataatag agaaggtagc cctatagaat ataccatatc aatatttaga acagataaat
    14881 atcaatatga aatagtattg agtgagtagg tgaataatat gaaatgttta attaatggaa
    14941 agataatatt aaaaaatcaa atcttagaaa ataaagtatt agtatttgat gaaaaaataa
    15001 ttgatatagc tgatagtgta ccaaaagatt gtgaagttat agatgctgat ggaaaatata
    15061 tatctccagg gcttattgat attcatatac atggaaacat gggaaaagat acaatggatt
    15121 cgactgatga atctattgaa acaatttcaa aatcaattat gagacatgga gtaacatctt
    15181 ttcttccaac tacaatgact atggataaag aacatgttta tgatgcactt gaagttatta
    15241 aaaaagccca aaatagaaag cttgaagggg cacaagtatt aggagcacat ttagaaggac
    15301 cttttataaa tgagaattat aagggagcac aaaatgaaaa atttataata aattcaaaat
    15361 acgaatttat aaaagaatat aaagatgtta taaaagttat aacttatgct ccagaaaaag
    15421 atattgattt tgattttact agagaaataa aaagatgtac agatatagtt ttatctattg
    15481 gtcatagtaa tgcaaattat gaccaagcta aagaagctat aaatttagga gtaactaatg
    15541 taactcacat gttcaatgca atgactggtt taaaccatag agacccaggc gttgttggtg
    15601 cagcacttac aactaatgtg tacagtgagt taatagcaga tacaatccat ataaataaag
    15661 atttatttca atttatacta aataataaag ggaaagaaag actgatttta ataacagatt
    15721 ctatagaagc tggtggtctt gaagatggca attattctct tggaggtcag aaagtcatag
    15781 ttaagggtaa tgaagctaga cttgaaaatg gagcattagc tggaagtgtg ttgtctttaa
    15841 ataaaatggt atttaacttc ttagataata caaaccttaa agtgaatgaa gcaattaatt
    15901 tagcttcttt aaatccagca acatctttgg gaataaatga caaaaaagga agcttagaaa
    15961 taggtaagga tgcagatata gctgtatttg atgagaattt agattgtaaa atgacacttt
    16021 gtttggggga cgtagtatat aaaaatatat agtatacaaa acaaacttta aagacagtaa
    16081 aaacaatata aaaaataata aattaaactt acattagtta aagtagtagt taaaatttag
    16141 gaggaataag atgagagtat tagtgtgtaa agattatgat ggaatgagta aaaaagccgc
    16201 tgaaatgata gcagctcaaa tagttttaaa accaaattca atactgggac tagctacagg
    16261 aagtacacca gtaggtatgt atagagattt agtaaaaaaa tacaatgata atatagtaga
    16321 tttttcagat gtgatgagct ttaatttaga tgaatattat aagttaccaa tatctaatga
    16381 ccaaagctat gattatttta tgaaagagaa tctatttaat catgtaaaca taaagccaga
    16441 aaatactcat ctaccaaatg gaatggctga tgatattgaa aaagagtgta tgaactatga
    16501 agcttctata gatgcagctg gtggtataga tgtacaagta cttggaatag gtcgtaatgc
    16561 tcatataggt tttaatgaac ctgatactaa atttgcaaaa agaactcatg tagttgaact
    16621 tacagagtct acaatagaag ctaatgcaag attctttaaa tcaagagaag atgttcctaa
    16681 aaaagctgta agtatgggaa taggttcaat attgaagtct aagaaaatat tgcttttagc
    16741 ttcaggagaa gaaaaagctg atgcagtata taatactgtt tacggagata taactccaga
    16801 agttcctggt tctatacttc aacttcataa agacactata gttatagtag atgaagcagc
    16861 agcaagtaaa ctgaatccaa aagattataa attagtataa gaatagaatg tagtttaagt
    16921 aaaaaatagc tatcattgag taatcaaatt tatttaatga tagctatttt tatttaaatt
    16981 ttttacttct ttttatttaa attagttatt attgtttaaa ttacttgctt attacttttt
    17041 acgaaaactc gttatcaaga agtatccaaa taaaatgtta aaaattatta tgtttagtgg
    17101 gattctatca agcataataa aattacctat aattgggata ttaccaactg gaaacataaa
    17161 gaagaataca ggcatcagtg atagtaaaaa agatggaaaa caaagaagaa gtaatcttgg
    17221 aatatcaata taccaactac catccttttt gtattctcta atcaataatt cagttcctag
    17281 taataaacca ataccaccag aggaaattaa attaataaaa atgtatcttt taaagtcttg
    17341 catataacca gtgtgaaatt gagtatagaa atcataattt aatttaacgg ctaaaaaata
    17401 cataagcaat agaatgatta aaaataaaag atatctaaga gcttttttca ttttaaattc
    17461 tcccttcaga aatactttat tcagttttat tttgttggaa tctgatttta tcatttagtt
    17521 taagaaatcc ataatattag tgaagaatga aataagtatt atatatgaat aaatattttt
    17581 agaattaagg tgaactgtag tgcttaatat aactataata ttaattgttt gaatattcaa
    17641 taaaaagttt gaaaaatatc aattttgata taaactgaat aaatacagtt gaatattaaa
    17701 ttattatttt atcaaaaata tgcaaagtac tacaccttca aatatcacct aaagtaatta
    17761 aaaggcaatt aaagtgaagt agttcaagta tttctaaaat aagtttaata aattctataa
    17821 taatttattt tttgccttaa aattgcctta aaatccaaac aattttaaaa ctaatgtact
    17881 ataaaaagct ggtataattt aaatatagta aaaaataaga tttatcttat ctattgggat
    17941 ttaattattt aatcaatata tttataccat ttaatttaaa taaaaagtct attaaggagt
    18001 gagcttatga gtaagttaat ttatatagca gatgatgaag ataatataag aaatttggta
    18061 aagacattct taaaaaatga aggtcatgat gttatggatt ttaaaacggg tgatgaactt
    18121 ttagaacaat ttaatataaa ggagtgtgac ttagtaatac tagatataat gatgcctggc
    18181 tcaagtggtt ttgaagtatg tacaaaactt cgtgagaaaa gtacagtccc aataatcatg
    18241 cttactgcaa gagatacaga tatagattat attacaggga taaccttagg tagtgatgat
    18301 tattttacaa aaccatttag cccaatgtct ttggttatga gagtaaaatc tatatttaga
    18361 aggatagagt ttgaaaagaa acaaaattat gataaatata gtaactcgat agatatggaa
    18421 ttaaaatttg gtgatgtaat aataaataaa aagaataaaa tcgttacaag taaaaatgta
    18481 aatatagacc ttacaccaaa tgaatataac ctattgactt atttgtttga aaatatagat
    18541 agagcagttt ccagagatga attattaaac aaaatatggg gatacgatat agaagttgag
    18601 acaagggctg ctgatgatac agtgaagaga ttacgtaaaa aaattttaaa tacaaatatt
    18661 ttaatagaaa ctgtatgggg gtttggattt aggttgaagg agaaaagtta agatgaagaa
    18721 tggaaatgac aagaatagaa ttggaaagat taaatccaaa tttaaattaa agttctcaaa
    18781 gaaaaatata agcataaaaa ctaggatttt gttgagtata ctttgtttga taattatagt
    18841 atttatgata atattagtat cttttaatgt atttcttgat acatatataa aaacaactgc
    18901 gaatgaagaa ttgacaaagt ctacagaaat agtagaacat atggatagta attttagacc
    18961 tattaagcca ccacaagatg atggaaagct tcctcccaaa gtattatcta attttatgag
    19021 aaatgtacaa gataaagtta ggatggcaga aacacaatca gatgctgatg ctatggtagt
    19081 tgattctaag tataacctga tatttccaac aagagaggat gatttcttaa aaaatgttga
    19141 tgagatggag ctgattaggg agtgtattca aaatgaaaaa ttagatttaa attcagatga
    19201 aaatacacgg atatctacca gaaataagga ttattatatt tcaactgtaa aaattactag
    19261 tatatatggt gaacaagaca agtatcttat actttttata gacatatcaa aaactctaaa
    19321 tctagcacaa aagataaata ttgtattaat atctgtaatg tgttttgctg gaatattagc
    19381 aatatttaca gctgtgattt tatctgagaa aattgctaaa ccgataaaag agttgtgtga
    19441 gtttgcaaaa aaaatgggtc aaggagattt caaaagaacc tactttgact tttcagataa
    19501 agaattagta gagctctcaa ctgttatgaa taagagtgct gagtatctgg ataagtatga
    19561 taatgaacaa aaaatatttt ttcaaaatgc atctcatgaa ttaaggacac cacttatgtc
    19621 tattaaaggt tatgcagaag ctataaaata taatgttata gatagtaaac atgcaagtga
    19681 tataattctt gaagaaagtg ataggttaag tgatatggtt gaagatttat tatacatttc
    19741 taaaatagat aatataacta aagattttga acttgtagaa tgtgatttaa gagaagtact
    19801 ttcaaattgt ggagctaggc aaaatgtaag agctataaat aaggggatag aatttattta
    19861 tgaatttgat gaagaaatgg ttttatttga atgtgatgaa aaaaacattt ccaaagcatt
    19921 tatgaatctt atagaaaatg ctttgagata cgctaagagt gaaataaaaa tagtgtgtaa
    19981 gtataatcaa aaaaatatag ttgtgataat agaagatgat ggaattggaa tcaagaaaga
    20041 agatttacct catgtttttg aaagattcta taagggagta ggaggaaatc atggaattgg
    20101 attatcaata gttaaatcta ttgtaaataa gcatggtggg cgtatatacg tagaaaatgg
    20161 caaaaaagga gctaaattta ctatattatt taaattgtaa aaaaattaga gaaaatatag
    20221 agatgtttga agttgttttt tatatatatc tgttgtatac tgttgaaaaa agaacaatat
    20281 taagctgaca tatattacga tagttgacat ttaaagtaaa tgcttaaata tagatacgaa
    20341 aggaaaagta aaatctgaag tgtatttagg tttaagtaag cttcttattt tataaaaaat
    20401 atgtatgaaa gaacagctga tttttaatat tacaaattta gtaacggttg tatttgaagc
    20461 ttttgtcatc catatgtttt tatcagactt tttaggccag aaagaagggt atataaaagt
    20521 tgtaagatat gctaaactgg gttttataat atgtttagga ttctgtaatt taataacttt
    20581 aaaccctaaa atcacaatgc ctttaatttt catccttatt ttttcaacat catttttata
    20641 taaaggaaat ttaaagacac gattgtttac tactgtttta ttaagtatat tctttatact
    20701 gtcagaaatt gtagttacaa gtatttttgt tttatttgta aaagaaggct ttgaaataat
    20761 gcttgaaaat aatagtataa gagtattggc gacaatttta tctaaaattg tctttttgtt
    20821 gacttgtaaa attatttgtc tatttaaaaa ggatgtacat ttagatatgc ccataaagta
    20881 ttggctccct ttatttttaa tacctatatt tagtttattt ctgtctgttt ctatctttga
    20941 tgtaagtaaa ttttttagtc ttgaaagttt aaaatttcta agcttaatat ctagtgttgg
    21001 tattttatat attaatttta ttgtctttta tttatttaaa tttattatag ataaaactaa
    21061 actatctatg aaatatgaac tacttgaaaa agaaataatt cataaggaag aattaagact
    21121 tagtaatgag tgttataaaa ttattgttga acagactgat tctgtcgttt tcgaatggaa
    21181 tatcaaagaa aataaatcat ttgtatcgca ggcttggact gaaaaatttg gatataacaa
    21241 tgcatgtaag aatattttta aagaaattaa agataaagac ttagttcatt ctgaagataa
    21301 agctatattt gaaggttttc ttgaaagtat taaaaaaaag aatatgcaca atcaagcagt
    21361 atatcgttta aaaaaatcta atggtgaata tatttggtgt agaacatcaa ttacaagcat
    21421 ttataatgat gaaaatgaac ttttaagagt tgtcggagta attgtagatg tagatagtga
    21481 tataaaaaaa tatgaagagt taagaactag agcagaaagt gactcattga ccaatatata
    21541 caataaaggt acatttgaaa aattagtgga agaaactata gttatgaata caggtgataa
    21601 aaaagatgca ttattcatta ttgatttgga tgattttaaa gaaataaatg ataattttgg
    21661 tcatccattt ggtgattttg tactaaaaac atttgctgat aagatacaaa ctagttttgg
    21721 tagtaaagac ttagtcggaa gaattggtgg agatgaattt gttgtatata tgcaagatta
    21781 tgttacagaa gtaaatttgc ataaaaaagc taaagaatta aatagagtac tttcggataa
    21841 ttacactgat ttgagtttta gttttgatgc ttctgtcagt ataggtattg caaggtatcc
    21901 tcaagatgga acttcatttt ttgaattgtt taaaaatgca gatagagcac tttatagcat
    21961 aaaagcatca ggtaaaaatt catattgttt atttgaagaa gaactatatg tacagtgaat
    22021 ttgaatttag attttatgtg agagtaagat ttatatatac ttgtggctgt ctgatttata
    22081 aaatatgtta ctttgtatat ttttatatgt ttcagtaagg ttaattaaca taagcaaagg
    22141 tgtaagtaaa tttatattag tttaagttta cttacacctt tataaaaata caaataatag
    22201 attcatttac attcttaaat gattaaagct ctttaccttc taaacatgca taacatatcg
    22261 ttataattcc agtaaataag actaaaatgc cattcctaag attacagttt catcatctat
    22321 tactccaaat gtttttaaag taacttattt cgctttgtat cataataatt tatctccttt
    22381 tacattttaa cttctgctga gatttgagtt agatattcgt tagaatctcg cacacttata
    22441 tcatatagaa gatattcttc ataaacaaaa tccccaatac gtaaattatt ctgattaaaa
    22501 aaatttaata acttttcata agtcttgtaa atagtttcat aactcccttt atgataagca
    22561 gttacataaa gtccctttgg tttgattgat acaggatatt tcttgtactc tttattaact
    22621 tttgtaaata aatatgaata tgattcaaag tctttattaa ttatatcttc acctttaatg
    22681 attgagccaa tcgtaccata atcactcaat tcatagttgt ccataaagtt aatacattca
    22741 tataaaaaat tttcttcctc atctcctaaa aattgttctt tatatgtaac aggtgttttg
    22801 accaagtact cttcttcatg ttcttttaaa gtaatagtat tcgcatctat attacaggca
    22861 tttttagtga atgaaatagt agattccatt agagcttgaa tattatttag tttttcaatt
    22921 tcatttttaa tatcaatggt tttcttttct aaaagctcaa ttagtaaatt aggtgttctt
    22981 ttgtctatat aagctttaat ttcttttaaa ggcattttta cttccctaag tgaagaaatt
    23041 ataccaaaaa tgctaagttg atggtatgag tagtatctat atccattttc tttctttaat
    23101 tctggtgaga aaagacctat atcatcataa tggaaaagtg tttttttatt gattccacaa
    23161 atctttgcaa attctccagt tgtaaaataa ttttttgaaa cttgactcat aattataatt
    23221 cctccttgac tatcctgtta cagtatagtt tatcatagta ttttgtaaag gagtgtgatt
    23281 atatgaaaaa attaattcat tttttaaaac catatagagt tttaattgtt gtagttttga
    23341 tatttacgtt tttacaaaca cttggaactt tgtatatacc aacacttaca gcaaatatag
    23401 taaataatgg tgtagtaaag ggagatatag attatattgt aaaaacaggt ttaatgatga
    23461 tgatagtagc tggtataaca gctttatcag ctgtgttagt ttgtaaagta tcagcaaatc
    23521 tttcatctgg attttgtaga gatattagag aagcagtatt tataaaatca caagatttat
    23581 caataaatga ttttaataat ataggtacag catcaatgat tactaggagt actagtgata
    23641 ttactttaat aggtcagtct gtgtttatgt ttattcaatt ggtattacca gcaccaatta
    23701 taactgtttc tggtctattc ttagcatatt ctatagataa agcaatgaca attattattg
    23761 ttgtagtaat gtttttattc atgctttcag catttttagt tggtaaaaag cttataaagt
    23821 tgtttaaaat gatgcaaata aagatggaca atatgaatag ggtgttacgt gaggtagtaa
    23881 ctggagtaag agttataaga gcttttaata ggagtcgttt tgaaaaaaag agatttgata
    23941 gaactgcaat agattattct gaaacagcaa tttcaataaa taaaatattt gcagtactta
    24001 tgcctattgt aatgttaata atgaatttag gtatagtaag tattatttgg tttggtggga
    24061 tgagggtcag taatggaaat atggaaatag gtcatataat ggcacttgtt gaatattgta
    24121 tcttaatttt attctattta atcatgggag taatggtatt tatgtacata cctagagcag
    24181 gtgcatgtgc agatagagta aatcagatat tagatataga acctgaaatt gtagatggaa
    24241 atggtcataa agatactgtc tcagagagag gacatttagt atttaaaaat gtaacctttt
    24301 catatgcaca atcagaagaa cctgtgttaa ataatataac atttgaagca aaatctggag
    24361 aagtaactgc aattatagga agcactggct caggcaaatc tactattgca aatataatac
    24421 cacgtttttt tgaaattcaa tctggagaaa tatctataaa tggtcaggat attaagaaga
    24481 ttcctcaaaa agaacttaga gataagattg gatttgtacc acaaaaagct tttttattta
    24541 gtggaacaat agaagaaaat ataaggtatg gaaaagaaga tgcaagtatt gaagaagtaa
    24601 agcatgctgc aagtatagct caagctgatg aatttatttc tgatatggaa gacaagtatg
    24661 actcatttgt agcacaaggt ggaaataatt tgtcaggtgg tcaaaaacag agaatatcca
    24721 ttgcaagagc gttagttaga aagcctgaag tatatgtctt tgatgatagt gtttctgcat
    24781 tagattttaa aacagataaa agacttagaa aagcgttgaa aaatgaaatc aaagattcat
    24841 cagctataat tatagcacaa cgtataagta caatcatgga tgctaatcag attatagttt
    24901 taaatgatgg aaaaattgta ggtattggaa aacataaaga tttacttgaa aattgtgaag
    24961 tttataagca aattgcggat tcacaactta gtaaagaaga acttgcataa aatgaagaat
    25021 taatctgata tttggaggtg tatgatatga gtgaaaaaag aatagatgaa caagatatag
    25081 aagtacttag ttttgaagat ggcatgtcag cagaaaaagc taaggatgca aaaaaaacta
    25141 ctaagagatt attaaaatat atggcaaaac aaaagtttaa aatattaatt atttttattt
    25201 cagtattgat aagttcagcg cttacagttt tagcacctat ggttatggga aaagctattg
    25261 accaactatt taatggaata aaaactgcag tccaaacagg tacaaaattc agtgtgaatt
    25321 ttagtactat gggagggata gtatcaatac ttctagggtt gtacttaatt agtgcagtat
    25381 ttatatatat tcaacagtat attatgtcag gagttgcaca aaatctagtt cttagcatga
    25441 gaaaagattt aagtgataaa ctcaataaac ttcctcttaa gtactatgat tctcataaaa
    25501 agggggaaac acttagtata gtaacaaatg atttagaaaa agtagcagat agtttacaag
    25561 aaggattaat gcaacttatt acagcagtag ttacagtagt tggttctatt gtaatgatga
    25621 taagtataag tgtaccactt acaatagtat cagctataac attacttgtt tcattgggta
    25681 ttactgttgt aattgcaaga aaatcacaag aaagattctc tgaaaatcaa aaggcactag
    25741 gagagttaaa tagcaatata gaagagatat ttactggtca aatagtggtt aaagcattta
    25801 gtaaagaaaa ggatactata agaaacttta aaatattaaa tcaaaatctt tataatgcaa
    25861 gtagaaaagc acagttttcg tcatatgcaa tatctccaat tattagattt ataaacctaa
    25921 ttggctatgt tattatagca gttgtaggtg ggatatttgc atcaactgga gcaatgactc
    25981 ttggtagtat acaagcgttt attcaatatg taaatcaagc atcagagcct actacagaaa
    26041 tatcttatat tgtaaatatg cttcaagcag caatagcatc tgcagagaga gtttttacag
    26101 ttatggacga ggtggaagag ataaaggatg aagaatcttc aaaggttatt agcatgccta
    26161 aaggtaaagt acaatttgag catgttaaat ttggatatag tgatgatttt attcttatga
    26221 aagatattaa tatcaatcta aatgcaggag ataagattgc aattgttgga cctacaggag
    26281 ctggaaagac aactcttgta aatttactta tgagatttta tgagatacaa ggtggaagaa
    26341 ttactattga tggtgttaat ataaaagatt taaagcgtgg agaacttaga actatgtttg
    26401 gtatggttct tcaagataca tggctgttta atggaagtat aaaagaaaat attgcatata
    26461 gtaagtcaga tgccacaatg gatgaaattg taagagcagc aaaatcagca agagtagacc
    26521 attttataag gactttacca caagggtatg acactatatt gactgaggat gcttctaatc
    26581 tatctcaagg tcaaaagcaa cttctaacaa ttgccagggc aatactttca gacccatcag
    26641 tattgattct tgatgaagca acatctagtg tagatactag aacagaggta gaaattcaaa
    26701 aggcaatgaa taacttgatg aagggaagaa ctagttttgt tattgcacat agattatcaa
    26761 ctattcgaga tgctgattta atattagtaa tgaaagaagg aactattatt gaacaaggaa
    26821 gccataagga acttattgaa aagaaaggat tctatgaaga actttataac agtcaattta
    26881 caagtgaata tgatgaagat gttgtatgat tatcaagttt taggcaacta aatgattgat
    26941 aaatattaag ttaaatatag taacatgtaa ggttatatgg aataagagta tttctctatt
    27001 tcatataacc ttttttattt atcaaaataa gataaaattt cctttctaaa ttagattgaa
    27061 gaatgtattt atcaatataa aagtaaaata atcattatta ttagtgaagt aaatattgtt
    27121 tgccacatat atgccataaa gatgcactta ttttacggag atttaacaaa gtagtgtaaa
    27181 gtatactcta aatataaaat aaatcaaata gagttaaata aattgagagg tgatataaat
    27241 agtgaataaa aaattgaact atagatttga aatgaagcac aaaataactg aagcagatgt
    27301 gttagcttta aaatcaagac tctatccaat aatgaagaaa gatgaaaatg catctaaaga
    27361 tggaaaatat cttattagga gtttatattt tgatactcct gaagataaag cacttttgga
    27421 taaattaaat ggcgttgcaa taagagaaaa gtttaggata agattttata ataatgactg
    27481 ctcttatata aggttagaga aaaaaatcaa acattacaac atgacatcaa agctatcaac
    27541 taatttaacc aaaaaagaag taataaatat actaaataat gatattgaat ttctaaaaga
    27601 gtcgacaaat tgtttattaa gagaatttta tttgaaatta aaatgtgaaa gattagaggc
    27661 aaaaagcata gttgattaca atagagaagc ttttatttat ccagttggaa atgtaagagt
    27721 tacaatcgat agtgatatta aaacttctgt aaattcggta gatttattta ataaagattt
    27781 gccaactatt tcggtaattg atgagaatat gactgtattg gaggttaaat atgatgaatt
    27841 tataccagat tttataaagg atttaattca aattaataag actacctcta cagcagtttc
    27901 aaagtatgct tcaagtagat tatatattta agaataaagg ctatgattta tataaagata
    27961 tacataaata aggttaaaaa acaaataagt taaaaataaa taaaataaaa aataaataag
    28021 ttaaaaatag gaaggatgat tttaattatg acatttaatg atatttttaa atcaagtttt
    28081 atagaaaacg tatcaggttt ttcatttgtg gacagtgctt tagcactagg ttcagctttt
    28141 ttagtaggtt tgtttatata tatggtctat aaaaagactt atatgggaat tatgtattct
    28201 cgaccattca atgtgtcgct tgtagcactt acaatgctta caacttttgt aatattggcg
    28261 gttacttcaa atgtagtttt atccttaggt atggttggtg ctttatctat agtaagattt
    28321 agaacagcaa ttaaagaccc aatggattta gtatttctat tttggtcatt aggttcaggt
    28381 atagttttag gagcaggttt gattccctta gctgtcatgg gttcaataat tatgggattg
    28441 attctaatat tcttttcaaa taaaacaata tctgaaactc catatatatt gatggtaaat
    28501 tgtaataatg aagattcaga agatatagca actgataaaa ttaaaaaagt atttactaaa
    28561 tatcaaataa aatcaaaaag tgttacacca gaaaaaggaa tagaattagt gtttgaagta
    28621 agaatgaaag atggagaaac aagtcttata aatgacttga gtcaagtaga tggagttact
    28681 aatgcagttc ttgtaagtta taatggtgac tatgtagcat agagaggagt aatcttttat
    28741 gaaagataaa aaatttaccc ttcttatctc gattatgatt atatttttat gtgctgtagt
    28801 tggagtttat agtacatcta gcaacaaaag tgctgattta tatagtgatg tatatattga
    28861 aaaatatttt aacagagaca aggttatgga agttaatata gagatagatg aaagtgactt
    28921 gaaggatatg aatgaaaatg ctataaaaga agaatttaag gttgcaaaag taactgtaga
    28981 tggagataca tatggaaacg taggtataag aactaaagga aattcaagtc ttatatctgt
    29041 agcaaatagt gatagtgata gatacagcta taagattaat tttgataagt ataatactag
    29101 tcaaagtatg gaagggctta ctcaattaaa tcttaataac tgttactctg acccatctta
    29161 tatgagagag tttttaacat atagtatttg cgaggaaatg ggattagcga ctccagaatt
    29221 tgcatatgct aaagtctcta taaatggcga atatcatggt ttgtatttgg cagtagaagg
    29281 attaaaagag tcttatcttg aaaataattt tggtaatgta actggagact tatataagtc
    29341 agatgaagga agctcgttgc aatataaagg agatgaccca gaaagttact caaacttaat
    29401 cgttgaaagt gataaaaaga cagctgattg gtctaaaatc acaaaactat taaaatcttt
    29461 ggatacaggt gaagatattg aaaaatatct tgatgtagat tctgtcctta aaaatatagc
    29521 aataaataca gctttattaa accttgatag ctatcaaggg agttttgccc ataactatta
    29581 tttatatgag caagatggag tattttctat gttaccatgg gattttaata tgtcatttgg
    29641 tggatttagt ggttttggtg gaggtagtca atctatagca attgatgaac ctacgacagg
    29701 taatttagaa gacagacctc tcatatcctc gttattaaaa aatgagacat acaaaacaaa
    29761 ataccataaa tatctggaag agatagtaac aaaataccta gattcagact atttagagaa
    29821 tatgacaaca aaattgcatg acatgatagc atcatatgta aaagaagacc caacagcatt
    29881 ttatacttat gaagaatttg aaaaaaatat aacatcttca attgaagatt ctagtgataa
    29941 taagggattt ggtaataaag ggtttgacaa caataactct aataacagtg attctaataa
    30001 taattctaat agtgaaaata agcgctctgg aaatcaaagt gatgaaaaag aagttaatgc
    30061 tgaattaaca tcaagcgtag tcaaagctaa tacagataat gaaactaaaa ataaaactac
    30121 aaatgatagt gaaagtaaga ataatacaga taaagataaa agtggaaatg ataataatca
    30181 aaagctagaa ggtcctatgg gtaaaggagg taagtcaata ccaggggttt tggaagttgc
    30241 agaagatatg agtaaaacta taaaatctca attaagtgga gaaacttctt cgacaaagca
    30301 aaactctggt gatgaaagtt caagtggaat taaaggtagt gaaaagtttg atgaggatat
    30361 gagtggtatg ccagaaccac ctgagggaat ggatggtaaa atgccaccag gaatgggtaa
    30421 tatggataag ggagatatga atggtaaaaa tggcaatatg aatatggata gaaatcaaga
    30481 taatccaaga gaagctggag gttttggcaa tagaggagga ggctctgtga gtaaaacaac
    30541 aacatacttc aaattaattt taggtggagc ttcaatgata ataatgtcga ttatgttagt
    30601 tggtgtatca agggtaaaga gaagaagatt tataaagtca aaataagatt aaaattgaat
    30661 ataaataatt ttaatactgt taagtctaaa tatttactta gcagtatttt tttagaaaat
    30721 aatgtatttg gttaataata taaggtaata aattgataaa aattaaatac agcatattaa
    30781 taatacataa taaagtaata aaaaaactgt ttaagtataa taacttatat ttagacagtt
    30841 tttttcattt ataaataata taaatgaaat tgaaaacatt tgaaggatag aaattggttg
    30901 gataaatgta taaattactt caccaaaaaa taatgacaaa aattgtaact aagttgtaat
    30961 aaaatggtag ttatttagca taaaataatg tatcatagaa gtatattata aaattaataa
    31021 aataagattt ttgtaagaaa actgctggct tttgatattt agagtgagta gttaaattat
    31081 gtttaggagg ggtagaattt gttgcaaaaa gtattatgga aatttttaaa gctagtagtc
    31141 ggtttattta tatgttcagt tggtatagta ttgacgataa attgtaacct tggattatct
    31201 ccctgggatg tatttcatca aggactttca aatcatatag gaataactat aggaactgct
    31261 tctataattg taggctctat agtagttatt gctgatgtcg ttcttggaga aaatgtagga
    31321 tggggaactg tttttaacat gctactaatt ggattcttta tggatttact tttatatagt
    31381 aatttaattc cagaggctga tagtttattt gtaggaataa ttatgttgat attaggattg
    31441 gttcttttgt ctgttggaat ggtattttat atgggaagtg gtttaggaag tggacctaga
    31501 gatggtttga tggttgcaat tcaaaagaaa acaggaaaat cacttaaatt aataagaggt
    31561 actatagaag ttggagcatt aatagtagga tttttattag gtggaaaagt tggtatagga
    31621 actataataa gtgcatttgg attggggtat ttcacacaaa tggtatttgg cttatttaaa
    31681 ttagattgta gtaagattaa acatagattt atagtagatg atataaaatt tatcaaagtg
    31741 tatatgagtg gtgagaaaaa agctgctgtt caaaatatag ttaagtaatt taagaataaa
    31801 ttcaacgaaa tgttaagtat tttaaattaa cattttgttt agatagataa attgaatatt
    31861 aaaagaatat taaaatatga ggattgctag aacactataa aatatagatt tctagcaatt
    31921 ttttatttgt tcaatatttt tttagtacta gaagtcttat tctctattta atgaatgaat
    31981 taatttataa ttagagatac taaactttat agatattaat ataaacacct ttaaactgtg
    32041 tattttccaa agcctttaaa atgcttgtat ttattctaaa taaaaaaata aaaaaatgtc
    32101 aaaaaaactc ttgtattata tccaaatttt atatataatt acctatgttg tttagaaatt
    32161 ttaaactcaa tgtttagtaa acaaaatgtt tagtaaaata aaacagaaac taggaggtgc
    32221 aacatggata taggtgaaaa gataaaaaga ttaagaacag aaaaacaact gacccaggaa
    32281 gagttagcta atagatgtga attgtcaaag ggatttattt ctcaattaga aaataactta
    32341 acatcaccat ccatagctac ccttatagat atacttgaaa tactaggtac aaatttaagg
    32401 gaatttttta atgaaataga tgatgaaaga atcagtttta caaaagaaga tatgtttgaa
    32461 acagaagatg aagatttaaa atataaatta aagtggttaa ttccaaattc acaaaaaaat
    32521 gaaatggagc caataataat aacattgtat ccaggaggac agtataaaga agaaaagcca
    32581 catgaaggtg aagaatttgg atatgtatta gcaggttcaa tatatgtcca tataggcgaa
    32641 aagaaaaata aagtaaaaaa aggagagagt ttttacttta gaccgaaagc aaatcactat
    32701 atatcaaacg agggcaaaac tacagcaaaa gtgatttggg taagtacgcc accatcattt
    32761 tagaatagag gtgtttaata attggtagaa aatataatag aattaaggga tatatctaaa
    32821 cattatgaag aacttactgt tttagataat ttcaatttag atataaagaa aaatgagttt
    32881 ctaacattac ttggaccaag tggatgtgga aaaactacaa cattaaaaat aatagcagga
    32941 tttgagtatg ctgatgatgg taaagtttta tttgaaggta aggagataaa taatttgcca
    33001 ccatatgaaa gacaagtaaa cacagtattt caaaaatatg ctttatttcc acacatggat
    33061 gtttatgaaa atgtagcttt tggattaaaa attaaaaaga ctccaaaaga tgtaatagat
    33121 gctaaagtaa aagaggtttt gaaacttgtt gcactagaag gttttgaaag acgacaagtt
    33181 gaatcattga gtggaggaca acaacaaaga attgcaatag caagagctct agttaatgaa
    33241 ccaaaggtat tattactaga tgaaccactt ggagcacttg atttaaagtt aagacaagag
    33301 atgcaaatag aattaaagag aatccaaaaa aaattaggaa taacatttgt ttttgtaact
    33361 catgaccaag aagaggctct tactatgtca gatactattg ttgtaatgta taagggcaaa
    33421 attcaacaaa tgggaacacc acaagatata tacaatgaac ctaaaaattc atttgtagct
    33481 aagtttatag gtgaaagtaa catatttgat ggaattatga tagaagatta taaagttaat
    33541 ttctgtaata gagattttga gtgtgttgat aaaggattcg aaaaaaacga aaatatagaa
    33601 gtagtaataa gaccagaaga tataaaaatg gtaaagccag aagaaggaat gttaaagggg
    33661 aaagttacat ctacagtgtt taaaggagtg cattacgaga tagaattgaa tgaaaatgga
    33721 agattgtgga tacttcataa tactaaaaat gctgaggttg ggactgaact aggcatggat
    33781 atatatccag aagatatcca tataatgaga aaagagaata actaagatga aaagaaaatc
    33841 ttttttagca tatccatatg tagtttggag tgcgatattt gtaataattc ctttaatatt
    33901 agtagtattt tttagtttta caaaagaatc aggtggaggt tatgccttta cacttgagaa
    33961 ttataaagaa gtaattgacc ctatatatat gaaagttttt gggcgttcta ttcttttagc
    34021 aggaggagct acattaatat gccttatagt tggttatcca gtggcatata taatttcaaa
    34081 ggctcgtgta agtagaagag gttcattaat attattgttt attcttccta tgtggatgaa
    34141 ctttctttta agaacatatg catgggttgc tatattagga aaaaatggac ttttaaatac
    34201 atttttaggt tggtttggaa tacaaccatt agcaattttg tatactaatt ttgccatact
    34261 tttaggtatg gtgtataact ttttaccatt tatggttctt ccaatatata cagcactatc
    34321 aaagatggat aatgatttga taaacgctgc tcatgacttg ggtgcaaata atatgacagt
    34381 ttttagaaaa attatatttc ctttgagttt gccaggtgta atgtctggaa taactatggt
    34441 atttatgcca gcagtcacta cttttgcaat atcaagactg ttaggtggag gtaaaataat
    34501 gctggttgga gatttgattg aacaacagtt tacagttgtt ggagattgga actttggttc
    34561 agctatatcg atatttatga tgatagtaat attaatatct atgtctataa tgtcaaaatt
    34621 tggagatgaa tctgacaaag aaggcggtgg gttactattt taaaattaaa gaaaataact
    34681 tctaatatat atttagcatt agtattctta tttttgtatg cacccatatt tgctttggta
    34741 ctattttctt tcaatgattc aaaatcaatg gcaagatgga atggatttac atggaagtgg
    34801 tatggtcaat tacttcaaaa tgagagtata atgagtgctt tatattatac tattgtaata
    34861 gctatattag cgtctgtaat atctacaata gtaggaacta taagtgctat aggaatacat
    34921 aagatgagag gtaagagtaa gaaattaata ctaaatgtaa actatcttcc tattttaaat
    34981 acagagatag ttacagcagt agctcttatg agtctgtttg tatttgtaaa aatggaattt
    35041 ggatttacaa caatgctatt ggcacatata atgttttgtt tgccatacgt tatactttca
    35101 gttttgccaa aaatgaagca attacctgat aatatagaag atgcagctat ggacttggga
    35161 gcaacaccta tatacgctct tagaaaagta attttaccac aaataaaacc aggtatagtt
    35221 tctggatttt taattgcatt tactatgtca atagatgact ttataataag tttctttaat
    35281 gctggaaatg gtgtaagcaa tctttctata gaaatctatg gaatggctag aagaggtata
    35341 aaacctgaaa taaatgcatt atcaacaata atgtttgcag tggttctagg tttactttta
    35401 ctagctaata aaaaagaatc tatagtaagg gggattaaat aatgattaag tttaaaaaag
    35461 caatatccct tatagctgta ggtatgatga cagttacttt atttacagga tgtaataaag
    35521 caactgattc tacaaaagtt ttaaatgtat ataatgttgg agattatata gatgagtctc
    35581 ttatagataa gtttgaaaaa gaaacaggaa tagatgttca atattcaact tatgatacta
    35641 atgaaatgat gtatcaaaaa gtaaagagtg gtagtacaca ctatgattta gtatttcctt
    35701 cagactatat ggtagagaaa atgaaaaatg aaggtctttt ggaaaaatta gattttaaga
    35761 atatacctaa tatgaaatat ttagataaga gttttttaaa tcctgtatac gacaaaacta
    35821 atgaatatag tgttgcatat atgtggggaa ctcttggaat actgtacaat aagaaagaag
    35881 ttaaagaccc tatggatagc tggaatatac tttggaatcc aaagtataag ggaaatataa
    35941 tgatgtttga ctctgttaga gatactatag ggataactct taaaaaatta ggatatagca
    36001 tgaatagtgt aaatccaaaa gaaataaatg aagctaaaaa tcttttaatg aaacaaaagg
    36061 atttagtatt ggcttatgta aatgatgaag gtaaggatag attgcttggt ggagaagtgg
    36121 caatgggtat gctgtattct ggagatgcag ttacactaat ggagcaaaat cctgaccttg
    36181 attatgctat acctaaagag ggaactaata aatgggtaga tgctatgtgt gtacctacaa
    36241 cagcacaaaa taagaaagaa gctgagctgt ttataaactt tttattagac ccagagaatg
    36301 caaaagtaaa tgctgagtac attgggtatt caacacctaa tacaggagca ctaaaattat
    36361 tagacccaga aataactcag aatcctgttg catatccatc taaagaagtg cttgataaat
    36421 gtgaaacttt tgaagatatt ggtgaaaata taaaattata tgacaaggct tggatagagt
    36481 taaaatcaaa atagggtctt gttggtttaa gtaaatttac ttaataaata cttaataaaa
    36541 ctgttctttt ttagaaaatt tattttctaa ttattgaaca gttttatttt ttgtttaagt
    36601 tatattcata tgcaatttaa atagtttatt aataaatgaa aacttatttg tataatgact
    36661 ttaataataa ttagaaaaaa tgggcaactt aatagaataa ttggaggtgt aaatttataa
    36721 aaaatgaata ggataaataa aaaaatagat ttattaatat tggctttgct cgtttttata
    36781 tttttaattg ttggaatagt aatactatcg attaggacag aaaataaatt gaatgtgttc
    36841 ataatgttag caataatttt ttttataata atgttaactt atttatcaaa ttcagtagtg
    36901 gggttgataa caagttcaat aattatattt atgtatacat cctatatttt atataacaat
    36961 attactcaca atatggatgt tgaatttata tcatatatgt ggataatagc aacaccagta
    37021 tcttcaatta taatgggtaa tctgaacaaa agcataaatg aattgcaaaa tacaaataaa
    37081 aaactatctg aacagtataa ggaattagtg actattgata gtgaaactgg acttagaaat
    37141 ttaaagattt tttataatga tgtaaatatg gagataagta aatctattag acataatact
    37201 gatttttctc ttatgatagt aaaattacca tactacggaa acctacagac tatatttggt
    37261 gaaaacaaaa ctaataaaat agtaaaatat attggttcaa atattataga atgtacaaga
    37321 aatgaagata taatttatag tttacaaaaa gatatgatag ggatactaat gccaaataca
    37381 tctttggaag gttcaaaagt tgttaaagac agaataaaga aaaggataaa agaattaaat
    37441 ttagatttaa acaatagggg aaaatatgta aatatagatg tcaaaattgc atttttacaa
    37501 tataagagta gttttggaga tagtataaat tttaagaata tagttgaaga ggaacttcaa
    37561 tatgatgttt aaaatattta ataaaagtct attaaaatta gtagttgtat tatcaatagt
    37621 actgacaata tcaaatttta gcttatcttc atatgcatca gaaattactt ccagcacaga
    37681 taaggtatta atcatatatg attctaagaa agaaacagct tataatagag atattttaaa
    37741 tattatgagg actctattgg gaagatttag ttcagatata gagctattaa aattgagtaa
    37801 ctatgatggt gagataaata aaaattatta cagtcatata tttattttag gaatcaatga
    37861 aaactcatat aataatgata aaaacactaa aaacttgatt agttccttaa atttatataa
    37921 aggaactata tgttggttag gatatggtat agaaaatctt ttggaacata aaaaatacaa
    37981 tttagactat gtaggaaaaa caaacaatat agttagtgta aattatagag gtaaatcata
    38041 taacttggat gaacattatg tttttaacat tgtagaatca aaagatacaa gtaataaagt
    38101 aataggaagt attaatgata ccttaaataa gtatccatat ataataaatg ataaaaatct
    38161 tttttatgta tcaaaattag atttagatgg agttttattt tatatatttt gcgattcact
    38221 taatgatatt tttaatatta agacgtttga taaaggacgt atatttgtaa gaatagaaga
    38281 tgtacatgct tttagagaac caaaaaatct tgtagagata gcggattatt taagtagtaa
    38341 gaatatacca tttacgattg cattaatacc agcatatgta aatcctaaaa atcataaggt
    38401 aataacttta tcagaaagcc ctgaaattgt caaagcaata aaatatatgc aagataaagg
    38461 tggaactgtg atattgcatg gatatactca tcaatacaag aaagaagaag tttctggaga
    38521 agggtatgaa ttttgggatg gtaaaaaaga tgaaccctta aaagaaaata tgaagatatt
    38581 tgtcaaagat agagttttga atggtcttag agtatgtata gagaatggaa tttatcctct
    38641 tgcatttgaa gcgccacatt atgcaatgga atcagaggga tataaagaat taaaaaaata
    38701 tttttctact tatatagggc aacatcaaaa taatgataaa aaatttagca caaatacata
    38761 tccttatatt attagggata cagaggagtt taatatattt atacccgaaa atttaggata
    38821 tatagaccca gaagataagt ttacttttca aaatataaaa gaaaatttaa ataaattatc
    38881 cattgtaaga ggttttagtg gagggttctt ctttcattca tatttaaaca tagagtatct
    38941 aaaaaacaca atagaatatt tagaaaaaca gaatatagaa tttatgaatt taagagattt
    39001 taataattgg gtaaaagttg atgaaataca aataagaaat aatggagatg aaattatagt
    39061 caactatgat aaggacttag atgaactaac taaaagtgat actagattta aatccatatc
    39121 caatattagt aaaattttaa tattcatagt atctataagt gttttaatat ttgtgattat
    39181 atttatatat tttaagagaa tagataaaaa gaaattttta aaataaatga ttttatatta
    39241 gaatcaatat tttaaaatat tttaggagaa atttatgaga gatttaaatg tatcagagta
    39301 tttgttcgta tttagtctgt ttagtatatg gtctttattg cttataaata ttattttagc
    39361 tatgggtgga tatatatttt attttaaaaa ctttgataaa gaaataaaag aaattgatga
    39421 atatcctatg atatcaatac ttgttcctgc tcataatgaa gctaaagtga taggtagaac
    39481 tgtggagtct ttgctattac tcaattatcc taagagtaag atggaactta ttgttataaa
    39541 tgacaattct tctgataata gtaaagaaat tttagaaaat ataaaggata ggtacaacaa
    39601 ttataatttc actataataa atacagatag tttaacaggt ggaaaaggaa agtctaatgc
    39661 attaaatatt ggatatacga tttcaaaagg agattttata gctgtttatg atgcagataa
    39721 cacaccagat aaaaatgcac ttagatatct agtacaaact atagtaatga atgatgaatt
    39781 aggtgctgtt attgggaaat ttaggactcg aaataaaaat aaaaacctat taaccaaatt
    39841 tataaatata gaaacattaa gctttcaatg gatgtcacaa gctggtagat ggcaactttt
    39901 taatctatgt acaataccag gaactaactt tatattgagg agaagtatca tagaagaaat
    39961 tggaggatgg gatagtaaag caattgcaga agatacagaa ataagtttta ggatatataa
    40021 attgggatac aagataaagc ttgtaccaca atctataaca tgggaacaag agccagaaac
    40081 tgttaaggtg tggataaagc aacgtactag atgggcaaaa gggaatatat atgtattaat
    40141 gaaatatata aaaaatatat ttaaacaggg aagaaataag attgtgtttg atatagctta
    40201 ctttttttct gtatattttc tatttttaac atctgtaatt atatccgata ttttatttgt
    40261 tttaagtata tctaaactag tagaaatttc tattccaatt aacttttttc taatatggat
    40321 actatcttat cttttattta ttatagaagt gagtataagc cttactatag aaaaaggtga
    40381 agcaacaatt gaaaatatat tcatagtggc aataatgtat tttacatact ctcaactatg
    40441 gctgtttgta gctataaaag gaatgataga gtatctaaaa gatataattt ttaaaagaga
    40501 ggttaaatgg tataaaacag aaagattttg attcatgtac aggaggaatt aacatgaaaa
    40561 aatttattat atcaattata tctttagtat tgtttttttc aaatatatca cttatataca
    40621 aagtaaatgc tgatgagact aaagttaaaa attataagtt tgaaagagat ataactattg
    40681 atggtgttat aggaagtaat agtacttttt ttgaagtaaa taaaaattgg gatattgaag
    40741 aagttttatt gcatcttaat tttagtaaga gccaaatatt aaatggagat gtttcaagtt
    40801 taactgtatt gataaataat gtaccaataa aatctattaa attaaatgct aagacaaatt
    40861 ataaaaatac attagaagta ttagttccaa aagattatat aattcaagga tataatgaaa
    40921 taaaaataaa aacatacaaa acaatatctg ataaaatttg ccaagatgat tccaatactg
    40981 ggaactggat ggttattcac aaagaatcat atatatcaat tagatataag cagaaaaagg
    41041 tggaaaactc aataaatgaa tatccgtatc catatgcaga aattgaaaat aatcataaat
    41101 tagatacaac tatagtagta ccagataata tgactagagg agagactaca gcagtgttta
    41161 atttggcatc agcatttgga aaaataacaa aaaatgatga tttaaagtta gatgttaaat
    41221 tatattcaga aatgaaaaat tggtcagatg ataatattat atatattggt aaacctgaaa
    41281 atacagcaga agaaattttg gatattttaa gtataaagga acaaacccta ttatcttcaa
    41341 actgtataat caagcaagta gattcaccat acaataagaa taagaagatg atggtggtaa
    41401 ttggtagtaa tgaagatgat ttaataaagg catcaaattt attaatagaa aatagacttt
    41461 ctaaccaagt tttatcatca tcagtacttg taaacaaaga gactaatata aaaattaata
    41521 gggaacaaaa attaaattta ggtcatttaa cattaaaaga tttaggatat tctgactttt
    41581 tgttggaagg agcatttaat caacaagctt tgtttgatgt caaaatacca acgggtaagg
    41641 tattagatga tggttcaaag attatcttaa atttaagata ttctgacaat ttagattttg
    41701 aaaaatcttt agttacagtg tctataaatg atgttattgt gggaagtaaa aaattggata
    41761 ggtcacattc taataatgat aagttagaat taaaaatacc taaagatata gataataaaa
    41821 actattatca agttaaattg acatttaatt tgagtataaa aaattcgaat tgtgttacta
    41881 gagagagtaa taatccttgg gcatatgtat caaataattc ttatttggcg ttatctacga
    41941 aagaaaatga aaccttatct tttgaaaatt atccgtatcc atttgttaga gatgatgaat
    42001 ttaatgattt aactgtgata atgccagatt attcagggtc acaagctatg acctggatgt
    42061 ttagattagg tgttacttta ggtgcgaata taaattcaca taatggaaat ataaatgtaa
    42121 ttagaggaaa agaatttagt gacaagtata aggatactaa tattgtagtt tttggagtgc
    42181 cacataataa ttctgttata aagatgttaa ataataattt aaatataaag ttcgataaaa
    42241 attactccaa ttttatatca aatgataaga taagctttat tgatgattat ggaaaaaata
    42301 tttctaccat tcaattaata aaatcaccat ataataatca aaaaaatatc atggtaatta
    42361 gttcaatgaa tgaaaaaaac ttatatttag gaatggatta tttgttgaat aaaagtaaag
    42421 ttaatgattt aaaaggagat acattgataa ttgatgaata cggagaggta gaagatttag
    42481 cttataattt aaaacctaag aaagaagtaa aagattcaag ctggaatatg agtataaata
    42541 agacaactaa agtattttta atgatttctt ttattactat aattgtagtc atgatattat
    42601 caatgctata tataaaaaaa tataaaagaa gataatatga taaatttatt attttaaaag
    42661 aggtatataa tttgaggaaa tatatgaatg aatttaaatt aaaaagaagt attttaatct
    42721 cattaataat cttaattttt atatttatag tttactttgt aagtattgag ttaaaagata
    42781 ggagtctagt aaataatcaa tataaaggta agattaaatc agccaattta tctgtagatt
    42841 atgaaataaa tcaagttatg aaggatatag ataaattggg attgaataca gtaaatgtac
    42901 ctataattat aaatgtagag tccattaatt ctgatattat gtcaatagat aataatagta
    42961 aagaaaaggc aattaagtta ataaaaaaat taaataaaaa aggtatttca acaatattag
    43021 aggcatatcc atggattgaa aatggaaaat tgtatgagac tgactggaac cctataaata
    43081 aaaaaagatt tttttatact tggcaaaatg ccatattaaa tgaacttata attgatgtag
    43141 caaatccact ggatgtaaat gttttaaata ttggttctaa ttttgtacat ttggaagaat
    43201 atcaacaaaa ttggggtgaa ataatagatt ttgtacaatc caaatttgat ggtcttgtaa
    43261 cgtacagaac taattggtgg tatacaaaaa aagatgattt aagtagtaag ttgttttatg
    43321 aacaaaagtt gaacaataat ttttttgata agttagattt tatatctata gcagcatatt
    43381 ttgagttaag taataaacct gttaatacag tggatgaatt aatttctgcc ttacactcat
    43441 ctactgtaaa caatagagga caaaatataa aacaagaaat atataacctt tataaagctc
    43501 atagaaaacc tatatttttt ggagaattag gtttttcaaa tagagaaagt gcaagttctc
    43561 agccgtggaa tcatactcct tcaaaagctg taaatggcga agaacaagca aggtgttttg
    43621 aggcatataa gaaagttttt gaaaatgaag actggataaa tggtttttct gtgttttgtg
    43681 taggtaaaat agatgacgaa aaaaattttt acccatcaaa agagagtata aaagttatta
    43741 agtcttggta tgaataattt tgaaagtgta gaatttatat agattaaata gatgattagg
    43801 agagatataa atgaatgaca tcaaggttat gactgtattt ggaactagac ctgaagctat
    43861 caaagtagca cctttaataa aggaacttga aaaaagagag aatataaata gtattgtttg
    43921 cgttacagct caacatagag aaatgcttga ccaagttata gaaaccttta atataaatgt
    43981 agactatgat ttggatataa tggaaaaagg acaaagcctg aatgatataa catgtaagat
    44041 tttaaataaa cttcccttga ttttaaacaa ggaaaatcca aatataatat tggtacatgg
    44101 agatacaacc acaactctag ctacaagttt gactgcattt tataataaaa ctcttgttgg
    44161 tcatatagaa gctgggctta gaacctatga taaacattct ccttttccag aggagttaaa
    44221 tagacaactt acaggtatta ttgctgatat gcactttgct cctactaatt tggctagaaa
    44281 aaacttgatt tctgaaggaa aacctaataa taatatattt gtaactggta atactgctat
    44341 tgatgcttta aagatgacaa taaaagaaaa ctataatcat cctataattg atgagattgg
    44401 taacgatagg atgattttgt taacctctca cagaagagaa aatttgggta aacctatgaa
    44461 aaatatattc agagctataa agcgaatagt agatgacttt gaagatgtac agatagtata
    44521 tcccatacat ttaaatccta aaatacgaac tatagctgat gagatatttg gaaaatttcc
    44581 tgaaaaaatt catattatag agcctttaga tgtagctgat tttcataact ttttaaataa
    44641 atcttatatg ataatgactg atagtggagg aatacaggaa gaagctccat cattaggtaa
    44701 gccagttctt gtgttgcgtg ataaaacaga aagaacagaa ggaattgaag caaagacact
    44761 taaattagta ggtactaatg aagatagaat atataattca gtgagcgatt tattaataaa
    44821 taaagataac tatgttcaga tgagtaaagc atctaatcca tatggagatg gaaatgcaag
    44881 caaatatata gtggatataa taattaaaaa atttaattgc aaatatttaa attagataag
    44941 gcctaaaaca ttaactaatt gatataagat ttatgataat attaaggaat acaattttta
    45001 agggggagta atatggctag aaaattaata aaaaatttgg gtaaaagtaa aagtgtaaag
    45061 agagtgaaac ttttatttaa gaagattttt attactgtat tcatagtagc aagtatagtt
    45121 gctattttta atataacgaa atattttgag gaattataca aagtaaggga tttaaaaagt
    45181 actaaaattg aatactatat ggatgtggct gatgaagcag gagatggaaa ggtccaatta
    45241 agttggaagg ccttacttgc tatagacatg gtaatacatg atgaagattt aagtaatata
    45301 aaaaagaaag acacattgga tataggggaa aagtttatag tagaagataa aaatgataaa
    45361 ggcgaaaagg tgtataaggt aaaaaagttt aataaggtat taagcgaatt gaaatttgac
    45421 tcttctcaaa aaagtagagc aagaaaatac atgaaagatt tggaatacac ataccttgga
    45481 aataaacaat tagatagtag tgatgaaaaa attaaattta taaagaagtt agaagactca
    45541 gctataagag aatatattga ttatggaata ttgccctcta taacaattgg acaagctata
    45601 ttagaatctg gttggggaaa ttctaaactt acaaaacaga gtaataattt atttggtata
    45661 aaagcagata aagcatggaa aggaaaaagt gtagaaattt caacttcaga gcattataat
    45721 gaaaaaattg tagctagttt taggtcgtat aattcattac aagattctgt caaagaccac
    45781 agtttatttt taattaataa taaaagatat agaaaacatg gattgtttga ggcaaaagat
    45841 tatattagtc aagctcaatc attagaaaat gcaggatata gtacagcaga agataaaaaa
    45901 ggaaatcgta tatatgcaga gctattaata gatgttatta ggagttataa tttgcaattg
    45961 atagataata aagttgagac aaaataattg ttaactttat atgaggagat aacaagataa
    46021 gattcttttc tacaaaaaga atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact
    46081 tgttatttaa aaggtgatat tattattact gtactcgaaa gagaacaaaa
//
LOCUS       DAMJYC010000026        43354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJYC010000026 DAMJYC010000000
VERSION     DAMJYC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.027"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..659)
                     /locus_tag="PC472_001544"
     CDS             complement(<1..659)
                     /locus_tag="PC472_001544"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2726874.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     I"
     gene            complement(1282..1821)
                     /locus_tag="PC472_001545"
                     /pseudo
     CDS             complement(1282..1821)
                     /locus_tag="PC472_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(1898..3703)
                     /locus_tag="PC472_001546"
     CDS             complement(1898..3703)
                     /locus_tag="PC472_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2726875.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4099..4476)
                     /locus_tag="PC472_001547"
     CDS             complement(4099..4476)
                     /locus_tag="PC472_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2726876.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            5381..5866
                     /locus_tag="PC472_001548"
     CDS             5381..5866
                     /locus_tag="PC472_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726877.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6054..6593)
                     /locus_tag="PC472_001549"
     CDS             complement(6054..6593)
                     /locus_tag="PC472_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2726878.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(6586..6999)
                     /locus_tag="PC472_001550"
     CDS             complement(6586..6999)
                     /locus_tag="PC472_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2726879.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7035..8156)
                     /locus_tag="PC472_001551"
     CDS             complement(7035..8156)
                     /locus_tag="PC472_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2726880.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            8755..10407
                     /locus_tag="PC472_001552"
     CDS             8755..10407
                     /locus_tag="PC472_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2726881.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTNQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            10706..11455
                     /locus_tag="PC472_001553"
     CDS             10706..11455
                     /locus_tag="PC472_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726882.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            11468..13495
                     /locus_tag="PC472_001554"
     CDS             11468..13495
                     /locus_tag="PC472_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726883.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            13639..15222
                     /locus_tag="PC472_001555"
     CDS             13639..15222
                     /locus_tag="PC472_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726884.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTTKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15194..15814
                     /locus_tag="PC472_001556"
     CDS             15194..15814
                     /locus_tag="PC472_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726885.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGGTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16009..16935)
                     /locus_tag="PC472_001557"
     CDS             complement(16009..16935)
                     /locus_tag="PC472_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2726886.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNNESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            17545..18465
                     /locus_tag="PC472_001558"
     CDS             17545..18465
                     /locus_tag="PC472_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2726887.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            18551..20971
                     /locus_tag="PC472_001559"
     CDS             18551..20971
                     /locus_tag="PC472_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2726888.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPISDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSNKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLSAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVYFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPNLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKALKENP
                     KSITGRYL"
     gene            20971..21993
                     /locus_tag="PC472_001560"
     CDS             20971..21993
                     /locus_tag="PC472_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2726889.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            22127..22873
                     /locus_tag="PC472_001561"
     CDS             22127..22873
                     /locus_tag="PC472_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2726890.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            22959..24209
                     /gene="cme"
                     /locus_tag="PC472_001562"
     CDS             22959..24209
                     /gene="cme"
                     /locus_tag="PC472_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2726891.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(24726..25739)
                     /locus_tag="PC472_001563"
     CDS             complement(24726..25739)
                     /locus_tag="PC472_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726892.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKYSILKGFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(25742..26770)
                     /locus_tag="PC472_001564"
     CDS             complement(25742..26770)
                     /locus_tag="PC472_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2726893.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(26770..27508)
                     /locus_tag="PC472_001565"
                     /pseudo
     CDS             complement(26770..27508)
                     /locus_tag="PC472_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(27576..29285)
                     /locus_tag="PC472_001566"
     CDS             complement(27576..29285)
                     /locus_tag="PC472_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2726894.1"
                     /translation="MPRRRKSQESRLTNNRTIWKVAVYIRLSREDGNDESLSVINQKK
                     ILKEYLEEMFEDEYVLVDFYVDDGLTGTDDERESFQRMIKDVKQGKVNCIICKTLARA
                     FRNYSDQGYFLEEFLPLHRTRFICLGSPKFDTYLNPEVITDGLDVPITGLMNDRYAAR
                     ASADIRRTFNTKRRNGEFIGAFAPYGYKKSPDNKNKLIIDEEAAQVIRDIFRWRVEEG
                     INKLGIVRRLNELGTLTPTKYKHSKGLMLKNPKLDKQDGMWSMNTISTILKNRMYIGD
                     MVQGRQRVISYKVHKTICTPENEWFIVENTHEAIIDKDTYLKAQELDQRDIRMSPKGR
                     NVYLFSGFLRCADCKRSMTKKTNKNKLNDGTVKEYNYYICSTYALKNKSKCSRHSIKL
                     EILEEAVLKAIQIQIAMVENMSEVISEINKQAIIVNQSSRLKKQLNEKQKELEKIICV
                     TDNLYMDWKSEEISRNEYARMKSRFEIKAEEIRRIISNIEAEIQSSSKGVGDNDPYLK
                     TFLKYKNIKELNRGILIELIDAIYIHENNEITIQFKFADQYKRLKEFIKDNHFSKKLK
                     YNN"
     gene            complement(29342..29584)
                     /locus_tag="PC472_001567"
     CDS             complement(29342..29584)
                     /locus_tag="PC472_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726895.1"
                     /translation="MSVNIVKKDNEKLNKKYIDIDKVINKQKDLEDEDLERCLKEMKS
                     PYCFICGDVVVRLKFSDSENTLDLCLISYFKNLKNC"
     gene            complement(30175..30414)
                     /locus_tag="PC472_001568"
     CDS             complement(30175..30414)
                     /locus_tag="PC472_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726896.1"
                     /translation="MSLVHKNNKLLPYDIIYAAAKGDIIAIGIVLKNYEAYIVKLCTK
                     TLYDKRGNAYKCLDLEKKNLLELKLIYKILKFKFV"
     gene            complement(30398..30835)
                     /locus_tag="PC472_001569"
     CDS             complement(30398..30835)
                     /locus_tag="PC472_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2726897.1"
                     /translation="MKNSSINKESTIHKFDSFCKKILKNESIDIERKNRYLRQVQILF
                     CELSEEQKNSLFSNDLYLSDYYSFNVNGFKIHIVNEALGEAIKLLPELRQKIILLSYF
                     IGYNDKEISGILNVSQDTVWYQRNVAIKTLRKILGVKKNEFGT"
     gene            complement(32006..32491)
                     /locus_tag="PC472_001570"
     CDS             complement(32006..32491)
                     /locus_tag="PC472_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2726898.1"
                     /translation="MTELWDLYDGERKRTGNVISRNSPIPNGLFHISVSVWIRNSKGQ
                     YLISQRHSQKTYPLYWECTGGSIISGEDSLAGAVREVKEELGIYLNPLGGKIIYQTCR
                     TKTKDFYDVWLFFKDLDIEEICLQDKEVIDVKWVDRGELVKMYNDGKLHPLIDYINIL
                     P"
     gene            complement(32494..33150)
                     /locus_tag="PC472_001571"
     CDS             complement(32494..33150)
                     /locus_tag="PC472_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2726899.1"
                     /translation="MYFEVAIFDLDGTLLDSMYAWERINNSFLEKRNLVVPDSYMNDI
                     CHRSLIEAAQYTIDLFNLNEAVEDIIEEWNESALVEYSNNVKIFEPAMEYIIKLKSMG
                     VKLAIATGLSKELYFPCLKNNNVLELFDVLCSTDEVSYRKEHPDIFRLVSVKTGVDPE
                     KCIVFDDILPAIKSAKKIGMQVCGVYDKYSEKNQNEIKSIADMYILDFNSAPLPYKGV
                     "
     gene            complement(33435..33653)
                     /locus_tag="PC472_001572"
     CDS             complement(33435..33653)
                     /locus_tag="PC472_001572"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2726900.1"
                     /translation="MAIVRQSGCEESTLLNMIELLEESDEGKIKINGKEFSKIESRAT
                     TILRRNTINYLFQSFALINDMIIYKNIF"
     gene            complement(34074..34232)
                     /locus_tag="PC472_001573"
     CDS             complement(34074..34232)
                     /locus_tag="PC472_001573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726901.1"
                     /translation="MKELKDIMVYESKKESKANNISLIYELIGKELNVDNIDEIVFRN
                     FKILLKNK"
     gene            complement(35722..36033)
                     /locus_tag="PC472_001574"
     CDS             complement(35722..36033)
                     /locus_tag="PC472_001574"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HDF2726902.1"
                     /translation="MYGGADYDSNENLKGLYLSDSDDYNSMFKDKKNNNNKYSMKRYN
                     VENKNGKLRLSTEDKEGFWVEVLELYSLSLGKDTWDKYFKNNNSCKEKVNNIGEIKIL
                     E"
     gene            complement(36017..36406)
                     /locus_tag="PC472_001575"
     CDS             complement(36017..36406)
                     /locus_tag="PC472_001575"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HDF2726903.1"
                     /translation="MRDLLNSYDKEKHSSKIFNLFKEYFGRWRRGIWADTTIDMFING
                     YIPSLTGENRGQRNDKNKFSTSKKDHRGGFFRDVFKEEILTERDKINNYEDLSKLVKK
                     RLGEGRVLAISHMMGSNIAHIVTVWGG"
     gene            complement(36403..37041)
                     /locus_tag="PC472_001576"
     CDS             complement(36403..37041)
                     /locus_tag="PC472_001576"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HDF2726904.1"
                     /translation="MSRSKRLIKNNLAKTITTTVMATGVSLSFGLLTVYAEELRTFDK
                     QVIETKSGEIGIVGWRLEDKEGNTIEGYGLGAKKSYELSEKVKPEVATKLTNEIDNSY
                     IYKNEKEENIKTLWVKGIIPPRMGENGDFTKEIVGTKKYYVTSQDKGNGWYDANKSTG
                     NLSIDDFALCGGATASNMLHWWLEQNQDYVYKFIKENPKNGILEFNGEKREI"
     gene            complement(37366..39435)
                     /locus_tag="PC472_001577"
     CDS             complement(37366..39435)
                     /locus_tag="PC472_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDF2726905.1"
                     /translation="MTRSRKNLKNNRKRLIKNNLTKTITTTVIATGVCAPFSLTNVYA
                     EEIKSVDKQIEAKKIDEKYPETGITGWGLEDENGKVIEGHGSEAKKVDKKYPETGITG
                     WGLEDENGKVIEGHGSEAKKVDKKYPETGITGWGLEDENGKVVEGHGSEAKKTDKFSE
                     QLKVNTEGEKDKIKTQNESNKEYIFEIFDLKKLQEIKDKLDKYKGKLNKELSIGLERQ
                     KSNIEIYIGLFKANNDCSNFKDLVKKPDVDENAYYRILNFINEEYDKFKEACDKYDRE
                     QTGKKSEQGKQPEDKKPDHSELEESEIAIEKPLKDIKDKALSDNARLHSEVDSLLDKY
                     GSVLGLDKSGIAAEFGAIEEIIKNADSYQKILTVGSMSECVKKEYDVTVVSNFLEKEL
                     KKIEKVININTETEENKELLREVRDISKKVLELNKLKGFIRELNGKNDTLIAKDEFEE
                     IYKNIREIKEKYNSIFDEGYVEKARLLYMKLKNGNLEKDQSSIGKERAEVMDKIRKSK
                     NKEELVDILKKPIPGNIDEFDKQYMESILLRTTNDELESQKNKLLDLLSRERGNLRWE
                     GIKEEDKQPEVKKPEQDKKPEMKQPESSKPEQDKKPEMKQPESSKPEQDKKPEMKQPE
                     DKIVESNKKKDHTQEANDPVKLYKKQEIKDNLNPKTGDSGTLGFAALGFSGLLGLVLN
                     KIKFRKK"
     gene            complement(39797..40033)
                     /locus_tag="PC472_001578"
     CDS             complement(39797..40033)
                     /locus_tag="PC472_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726906.1"
                     /translation="MKVENKNYRLLPYSVILEASKGDISAIEVVLKHYEMYITKLCTR
                     TVCDKQGRTFIYLDENKKSRIELKLITKILTFKP"
     gene            complement(40278..41969)
                     /locus_tag="PC472_001579"
     CDS             complement(40278..41969)
                     /locus_tag="PC472_001579"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019874.1,HMM:NF025854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HDF2726907.1"
                     /translation="MSNMRKKRNIYIAIVLICISSIGVLMYKYFIDNKKEYQERLKDT
                     NEIVIDTQKKRIKEIVDRTINDIELEEKRVINTKEKEIEEISLLFDSFSQDLETEKIV
                     NLVQKVGNKFDENSKVKLIIWNGARKSIEYSNDKNINDNKDINKEDILKYTKDNYILR
                     KVKNIGNSKLLILGVCQSDIDDRVKQIAIKKIRDTKLDEGGYIWVNEILNYNGGDNYA
                     KRLVHPNLIDREGEFLSTNMKDSKGKMPYLKELEDIKAKGESYNEYNFKKVNSNDESL
                     KLSYAKLYPKYNWVIATGVHLDDLQNYNKKYNQEYTEKFKKQFFFRVMNIILLFIYAI
                     ITGGIIFNYNEIRKKMIIKEKNKLLKSHYDILSDKNDDANKIIHDIKNHLITIYTLAK
                     EDGNNKLIEYLYSVGKDIKKYGNKVITENKIVDIVLNEKIELMKKKNIKFTHNIEKID
                     LDFIENKDLVGILSNLFNNAIESAEKCSERKIEFILYSFNKGYVIIKMINTCEKEPIV
                     KGGKLITTKGRKEYHGYGVRIMESIIEKYDGDIEWEYNDEKNIFSVLIMIPIKEI"
     gene            complement(42000..42707)
                     /locus_tag="PC472_001580"
     CDS             complement(42000..42707)
                     /locus_tag="PC472_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726908.1"
                     /translation="MCKIAICEDEKVQVEKICEILKKFELNEGIKLNVDTFSSGEELI
                     ENKFEKYDIVFLDIKMKKLTGIETSKIIRKSSEKIKIIFITALEQYWPEGYTVSAYRY
                     ILKPIKEDELYSIIKDLINEINKNKRFILLKKDGAIEKVLISDIKYLGIENRKVVVYT
                     VNGVYSTSISLGEWESKLYGYDFAKPHSSYLVNFKYIKQVNKNHVVLTDGESVHVSQR
                     KYKEFKDKFIKYINEIR"
     gene            complement(43067..>43354)
                     /locus_tag="PC472_001581"
     CDS             complement(43067..>43354)
                     /locus_tag="PC472_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726909.1"
                     /translation="KEGTIVETEENGCNGIVEIDKNKQEKSKDSNSVVNNKNEDKKDN
                     LSKQNTSINKLKESPATGDENIIIYIFSILMVIAFLLKKYIISFTKSLLKR"
BASE COUNT        14807 a         6869 c         4915 g        16763 t
ORIGIN      
        1 tctatagctt ctttagccat agttccatat ccttgcatta tccatgctgg tatttcttca
       61 tatccgtccc acgctgtatc ttgaaccatt actccatggt catcagcttc tgcataatca
      121 ttagctaatc ttactgcatc atcatagttt aagtctgtta tactagcatc tgcaccttct
      181 cctttgattt tatttaatct aaattcagaa gaaccttttg gcattaatac aacagatttt
      241 tgacctaatt tattagctgt ccaagcaact cctctaccat gatttccatc tgtagctgta
      301 aagaaagtta tttctcctaa tttttctttt atttcttttg aagttaattt atcataccct
      361 aactcagata tatctgtatc taatctttga gctaagtatc tacccataga gtaagaacca
      421 cctaaaactt taaatgcgtt taaaccaaat ctataagatt cgtctttaac atatattccg
      481 cttacgccta gtgattttgc taagttatct aatttaacta aaggagtttt ttcatattgt
      541 ggaaagcttt catgaaaagc tttagccttt gcaatctctt catcttttaa aaattcaatt
      601 cctttttctt tttcctctga tttaggtaag ttatttaatt tccattttat ttctctcatt
      661 tattttatat tcccctttca aatttttcgg tgtcatttta attttttatc tttgtgcctt
      721 atagcaaagc aattaccgtg ccaatattat ttattctaaa aacaattttt ttatgttgga
      781 attacatact tttataaaat ttctttaaag tttataaatt ttgagtgtga aaaataagat
      841 ttataaaaga aaaaattctc atattgggaa atttttatca atacgagaat tatcaattcg
      901 agaaatcgtt tatttatctt aattcttcct ttattatgct cttatttttt atatgtctat
      961 tgttttgtca gatatattac tacttttatt ttctataaat atatggctat aaccttctta
     1021 cgcataaact ctataaattt atatacttta tattctttat tacccaaata ttctttatta
     1081 cccaaagcct ccaaaatggc ttaaatcttt tcatagcttc ttacagaagt ttacatgggc
     1141 gtccccccaa taagcaaaag tgcaatatgt gtaccattgt gccctacttg cgactattga
     1201 gctgtcgaaa ttcgcaactt cgcatttatc tgatgaaatg cactatacaa aatattcata
     1261 acatccacaa cagagttaaa gttacctatt catattcact gtaaaaattt cttccacagg
     1321 aaatgtaatt ttaaccaatc ctattgagac aaactcacca cttatttttt tgaactcacg
     1381 cagtaataca tcacttgccc gaaaaaaagc ctcactttca tcatctccag taagtgctat
     1441 tgtgcaatgg ggtacccaac gattggggtg gtaccattcc caaccatttg aatcaaaatc
     1501 ttgtagtacc tcatataatt ctctctgaaa ctgctacata ctgctattca ttataggtga
     1561 tacaaacacc gttttagtat cattaaacat gcctactgaa ccaatgtaag caggtattac
     1621 cttatggttt tgtgcaaacc cctttagctt gtctatacaa caagcctcat ccacatcatt
     1681 gaaacaagcc aaagtaagat gaggtcttgt tttccattct aaatacttcg tactaatttt
     1741 ttcatcagcg atttttttag ataaatcaaa aagttgcttt tccgtttttt tatcataata
     1801 cagctctatc gcatattgca taataacacc tccaccataa agtaaaaata cctatctata
     1861 aaaacacaca aatacctatc attttaatat tatattacta ctgttcctct tccaattttc
     1921 tatatagtgt agccaaacct atgcctaagt cttttgctat cttcttcttc gtctttgtat
     1981 ctcttccata cttattcaac aacttgttta tgtaagtcaa ctctaattct ttaattgaaa
     2041 gtatcctttc ttgattctct actatccctc caactatatc atcttcaaat attatattta
     2101 catccttatt tttataaata tttccattaa tattataata gtttaaaata tctaatggaa
     2161 gcatatcttt atatattatt ccatcatctc caaccaaatt catcatatat tcaactgcat
     2221 tttccagttc ccttatattt cccggccaat tatagttaat caacatattc attacatttt
     2281 cttctatttt atttaactga atacctaatt ctaaagaata tttagaagca aacttcatca
     2341 ttattgcctc aatatcatcc tttctttccc taagaggtgg caaatttata ggaattacat
     2401 tcaatctata gtataaatca tccctaaact taccttcatt tactaatttc aataagtttt
     2461 tatttgtagc agcaatcact cttatatcta aatctataag tttatttgac ccaattctcg
     2521 caaattttct ctcttgtaaa actctaagta actttgcttg taaaggcatt gacaaatctc
     2581 ctatttcatc aagaaatata acaccttcat ttgcaagttc aaatttacct actttccctc
     2641 cactactagc tccactaaat gcacctttta tatagccaaa tagctcactt tcaaggagat
     2701 tttctggaat agcagcacag tttatcgcaa taaatggctt gttccatctg ctaccttcag
     2761 catgtatagc cctagcaatt agctctttac ccgtaccact ttccccagtt ataagtacag
     2821 ttgaatttga ctttgctaat ttcttagttc tctcttttac ttttaacatt gcttctgaat
     2881 ttccaattat agactctata tcactatttc cccagccact attcactttt ccatgtcctt
     2941 tatgattgat atatgtcttg tcaaatatta ttattttgtc gtattgattt atatatggat
     3001 ataccggaat tattttggca tttatattat aaattttgtt atcaatatca atttctagta
     3061 attcatgtcc atttgaggaa gattcaacag atactatatt tactatattt tctattatat
     3121 ttttatctat atctaatttt tttagagcta tattgtttat ataagatatt ttgctattta
     3181 tgtctagtat tataacgcct ttttcaacat tttcaatgat ttctatcatc atatccattt
     3241 ttctactgct attttttgac gcttcatgtt cttctatttt catagaaatt aaatcgcata
     3301 tatgattgac aaattttaaa taattatcta tttttgcact tatttctact tttttagttt
     3361 tatctgtgct aatagcacca attactccta tagttcgatt attatacttt attggaactg
     3421 ctattattac tttatttagg catttcatgt aatgggaaca ttctatacaa agctgattat
     3481 cacatatatc caatacgact aattcctgac ctgtctgtat aacattgtca taaataaagc
     3541 cctcagtaac aacgctttca tttatctttg atttatacag acctgtacca ctaattctta
     3601 ttaatctatc gtcaactatt tcaatatcta catctaacac actagctact gtttcagtgt
     3661 attttatcat ttcttgttga aattctttta atttacttaa catagtttcc cccattagaa
     3721 tttaatttac caaattatca gtttagctat attttaacat atagtcactc tttaaaatat
     3781 atataaaaca ttacttattt atatatttgc gttaaaacag acaatattaa tttataaaaa
     3841 gtttgtttta tatagtaacc ttaattataa tatgcagaca ataaaataaa tcacaataca
     3901 ataataaaaa aaagctttcc tcatataaca aatatatttt tgacataaaa atatacatta
     3961 tatgagaaaa gctcaatagt taaatattta gatagatttt tatatagact tttttaatat
     4021 aaactttttt aattgtagac tttttttatt agtttttaat ttttattagt tttgttattg
     4081 gttttattgt tataatactt ataatactgc cattgcttca atttctaaaa ctgcatcttt
     4141 tggtaattga gcaacttgta cgcatgacct tgctggtgga ttttctttaa aatatgtagc
     4201 atatacttca ttaatatcat taaaatcctt taaatctttt acgaatactg tagtcttaaa
     4261 tattttatcc atacttgtac cagcttgttc taatattgct tttacattat ctaaagaagc
     4321 ttttgttgct tcttttgggt cagatactaa ctcgccagtc ttcatatcta ttggaagttg
     4381 acctgatgta gaaactatgt ttccaacttg aattgcttga gaatatggtc caattgctcc
     4441 tggagctttc tctgtagcta ttactacttt tgacatgact tcacctccta ttattaagtt
     4501 atatttacag gaaaataaaa tttcctaaac accaatatta atacgaacct ttagattaaa
     4561 tttcaaatct aaaacttcaa ataatcaata cttagatgat tatgtttatt tgtgtcttgt
     4621 taacttttat taaagcaatt aacgtgccaa actttattta tatataaaat tttgtgaagt
     4681 cattcaaaat agttacttct gaattttttc gtaaaaaaac gctctaaaat tactttttta
     4741 tgatgtcaat ttttttctca tatctagaaa tttaccaaca tgagaattct tgttttgaga
     4801 atttacagat taaagttatt ttttaacctt aaattctgaa cctcccatga ctaaagtcac
     4861 agggttcctg cttcatagaa ttttgcataa tcaagttatt acatatgaaa gaactatagt
     4921 tgacaatccc actattataa ataataaaac ctttacccat accttattta ccctagttat
     4981 catagagcct attgaaaata taacagctaa atataataat aaccaaaaaa ttatcaatat
     5041 ctaccactgt tgctgaaaat ccaaaaaact accttttgat agtattactt aacaatctaa
     5101 ttaatctcat aataaattta caacttataa ataaattgtt atattggccg tatctcaaca
     5161 actttctcct ttcctagttt taaatctttt atagaattgt accacgtcta agtaaatttt
     5221 catactatta agtcttattt ctaataaatc attagctctc attctatgcc caataatgta
     5281 agattgcagt aattttattg attttcatag ttttatttaa tatacttata ttaattattt
     5341 tcatatgaaa ttattattta tataggggga tttttttaat atgagcaaaa gtgaattaac
     5401 agcagaaaca acagaagaaa tgttagaagt actaagtggt aaagattatg atattgcatg
     5461 tcatttacat gaacttggta aatcattaga ttgtaaaatt gaaccaaaaa caggtgctcg
     5521 ttcttacaaa atagtatatt caactaagaa accaaaacgt agcttattta ctattgaatg
     5581 taatgaaaaa aaatggagag ttaaagcaaa tctttttcat ctaaatacat ataaagatgc
     5641 tgtggaggaa tgctctaaaa ctattaaaga tagtataact aaaactcgta cttgtaagaa
     5701 atgtaattca aagtgtattg gaggttcttc gtttgaatta gatggaaagt cttacctgac
     5761 ttgcatagga agtggtcatt attttgcaaa tatggaagaa atggattgga aaaacctaga
     5821 aaaattaatt actaaagaaa ataatattat gcaggaatct gtatagtttt ttaagggtaa
     5881 ttattaaaat atatgagcaa caacagatac ttaaataggc ataaaaaaat aaagggtata
     5941 ataaggatat agaataaaaa taaatgatag cacaaagagg ctgtctcaaa atacagataa
     6001 gcctcttttt attgttgaaa tgataagaat atattcaatt ttatacaaat atactacgcc
     6061 aacttaccca tttcatatgc ctgtttcatt gccttacttc ccttaatatc acctacattc
     6121 caagcaccta caccatatac tatacctttt tccttagaac catttagaca tgatgtaaat
     6181 cctctaaatc cttctattgt tctttccata tcagccacat tttcatctgc tgctgtaacg
     6241 ataaaataga aatctttatc acaaatttca gtatatctag agcaagttct atcaatcata
     6301 gttttcattt gcgcattcat agtataaaaa taaactggag tcgctaaaac aatcacatct
     6361 gccccaatca ttttttcaag aatttctccc atgtcatctg tctgtgaaca atttgcttct
     6421 ttttcataac aagcaccaca tccagtacaa tagttaatat ctttatcttt taaaaatatc
     6481 ttctccactt gatttttaga ttctaatacc ccttgtataa actggtcaca caataaatca
     6541 gaatttccac cttttctagg actagctgat actattaata catttttact cataagatac
     6601 tgcctccatt ttcttatttc tactgctctt taatactttt tctttctcaa taataccttc
     6661 ttcatatcta tcaatcttaa aatttaatcg ttccaaagtt ttttgcattt cttctattct
     6721 ttttgctagt tggtttctct gctctattaa aagttctttt ctagctttga tagtactgct
     6781 cccttgttga aacatagcta catactcaat taatgtctca atagaaagac ctgcacctct
     6841 catacacttt atgaaattaa cccactcaca atcttcttct gtaaaagacc taattccact
     6901 tttatttcta tttacaggtg gtattaaacc aattcgttca taatatcgta aagtatccgc
     6961 tgaaagctcg tattttttac taacttctga aatcgtcata catatcttct cctcttgata
     7021 ttgtatagat tgaattattt ctcaaaggtt tctgctacat tctttaaagc atctataatt
     7081 ttaatgtgtt gaggacagct ttcttcacat tgtttacatt caatacaatc tgaggccttt
     7141 ccataagttt tagtataatt attatagtag accatctgtg ttgaaaatcc agtattaaga
     7201 gcttgttttt ctgcattgta caacgcaaaa tagtttggaa tagcgatatt ttttggacag
     7261 ccttctacac aatactgaca ggcagtacaa ggaactaaaa ttgattcgtt gataatctct
     7321 actgcttcat ctataatatt atattcctct tgtacgaatg gtttgaaatt ttgcatatat
     7381 cctgtattgt ctaataattg ttccatattt gacatcccac ttaagaccat cataacatta
     7441 tctttacttg ctgcaaaacg tattgcccaa gatgaaattg acatatctgg attgtagtca
     7501 ttcaataatt tctctgcttt ttctggtatc tttgcaagtg tacctccttt tactggctcc
     7561 ataacaatta caggcttatt atgcttctct gccacctcgt agcattttct agattgtatg
     7621 ctttcattat cccaatctaa ataattaatc tgtaattgca cgaagtctac ttctggatgc
     7681 tcactcaaga ctttatccaa taattctgca ctatcatgaa aagaaaaacc aatattcttt
     7741 atttttcctt cttttttctt ttcttcaata aacttaaagc tatcaaattt tttagctact
     7801 tcataatgtg acacgcctat attgtgtaaa agatagtagt caaaatagtc tacaccacat
     7861 ttttctaatt gctcattaaa aatggtttct tgctcttctt ttttctttaa agccattaat
     7921 ggaagttttg tagctatagt aaaactctct cttttgtgtc tttttactag agattctcta
     7981 agtgctatct cactttttcc catgtgatac acataggctg tatcaaaata tgtaaatccc
     8041 ctttctagaa aagtgtctac cattttattt aaatgttcca tatcaatagt tgtaacatca
     8101 ttttcatctt ttattggtaa acgcattaat ccaaatccta atttttttgc ctccattgtt
     8161 ttacttcccc ctttaaaact tgtttgtttt ttctctctac tttaactatt cttacaagta
     8221 tataattctt gtataattat actttactac ttggagttaa cttcaagtca atagattttt
     8281 ttatttttaa gacaaatatt ataaaaatac agttctttaa tattaatcca ttacatgttt
     8341 ctttcaccct ccaattctca aacttcacaa actccaacat gtaaagaaat atatacctta
     8401 agaaattcag tccattaata ttactacaaa tactttttag atatgtttaa aaagaaattt
     8461 cacctaaaat atttataaaa gatttagaaa taggtcttgt gtctatttta aaatatataa
     8521 aagagtggta aagacttatt tataagataa aaaatgaata gtgtataccc tttttaaaaa
     8581 gttttttcct ttttatattt gtgatacaat gttaaatata aaaaggaaaa ttttgtatta
     8641 aagaaatctt atttgttgat tttgtaactt caaactcagc atcaatttga ttttaagatt
     8701 atattttatt aagtatgcta aataacaaat ttttaaagat tgtgaggtaa aaatatgaat
     8761 aagaaaaatt tagctatatt aatatcaggt atgatgtttt tttctagttc ttcagcaatt
     8821 tttgcagaca atactacaaa tcaagaaaga ttaataggta aaacaagata tgaaacagca
     8881 gtagaagtaa gtaaattggg atgggttcaa tcaaaaacag ccattatagt taatggtaat
     8941 tctattcaaa gtgcgctttg tgcaaatcca tttgctaagc taaagaatgc acctatactt
     9001 ttagtaaaca acaacagtat tgaaaattca actaaggctg agcttaaaag gcttggtgta
     9061 gataatgtgt acattgtaga tagtggaaat agcatttctt ctaaagtaga aaatgaaata
     9121 aaatcactta acataaaaat aaataaaata gtaggaaaca atatatatga aatgtctaca
     9181 aatgtactta aagaaataga taagataaaa aaggtagaaa atgttgcagt tgtaaatgga
     9241 ctaaagggac tatctgatgc agttagtata gcttctccag cagcaataca taatatgcct
     9301 ataatattag tatcagatga aaacagcaat gatagtagta taaatttctt aaacaatagg
     9361 tctataaaaa aatcttacat agttggagga aataagattt tatcagacaa tacagtaaag
     9421 aaatatccaa acagagaaag aatcagtggt tcagatagaa acaatactaa tgcaaaaatt
     9481 attgataaat tttataaaga taaaaaaata aataatgtat ttgtagctaa agatggtatt
     9541 aaccaagaaa atgcattagt agattcatta tcaataggag tactagctgc taaagaaaat
     9601 tctcctataa tgatatctaa tggtagcctt gtaaatagtc aaaaagaatt tattaaaaat
     9661 aaagatatag atgaaataac acaagtagga ggaggatata atcaaaaagc atttgaagat
     9721 attttaaaac taaaaaatgt gaacataaga aataaaacta aaaaaatatc tttaatacag
     9781 gctaaagaaa tattaaaaca agctcttcca ggaagcaata ttttagtttg ttattatgat
     9841 gaagatgata atgaatatga ctgcaaagtt ataaaggata atatcctata tgagatagaa
     9901 attgatgcct actctggtaa aataatagaa attgaaaaag atgatgactt aaatgataat
     9961 aatttaggta cacaaaatat atcattggat aaagctaagt ctatagcatt agatgccatt
    10021 cctaatggta atttaatcta ttgtaaatat gatgctgaag ataacgaata cgaggctaaa
    10081 gtaataaaaa ataagaaaac atatgaatta gagataagtg catttgatgg aagaattgta
    10141 gatattgaca tggacaatga ttctgatgat gactttgatg ataatgataa gaataactct
    10201 aatactaatg acaacagttc tattattagt gctgaaaaag ctaaatcaat aatgctagat
    10261 aaagtgcctg gtgggaaaat agtaaaattt aaatatgatg aagatgacaa agaatatgaa
    10321 ggtgaaataa taaaaggaaa aactgaatat gagataacaa ttaatgcaat tactggtaaa
    10381 atattagaat ttgaaataga tgattaataa aaaatatata ttctattaat tattacaagc
    10441 aataaagcca taaatattga aattttcaat atttatggct ttattttatt ttttttaatt
    10501 taaatccaca ttatgccatt atatatatct atgcactctt ttaaattaaa atcttgtcca
    10561 tcatcccaaa cctaataaag ttagatttta aacaatatat tgtgatataa gtaatttgtg
    10621 tagaattatt gcgtgattta ctcatgaaga aacaataagg ttaatatata atttgactat
    10681 attaagacga aaggagacat atacaatgaa tacaatatta aaagtaaata acataactaa
    10741 aacttataat aaagggcaac agccgagttt agataaggta tcctttaatg tatcagaagg
    10801 tgaatttata ggtataatgg gagcttctgg ctctgggaaa acaactctac ttaatgtatt
    10861 atctacaatt gataaaccaa caagtggaga tattttcata aataatataa atttgaaatc
    10921 gcttaatgac acttgtgctg ctgattttcg taaggataat ttaggcttta tctttcaaga
    10981 gtatttttta ctagatagtc ttacaatttt cgagaatatt tcagtacctc ttacacttca
    11041 gaaactcaat ccagataaaa tagaaaacat ggtaaagagt ctagctgaac gatttggcat
    11101 agcttctcaa ttaaataaat acccaaatga attatctggt gggcaaagac agagagcttc
    11161 agcagttaga gcaatagtaa aacatcctag tattctcttt gcagatgaac caacaggagc
    11221 tttggattct aattcagcta ctgaactact ccactcacta caggaagcaa atgaggagtt
    11281 gaaaacaaca attttaatgg taacacatga tgcctatgta gcaagttttg caaatagaat
    11341 tttgattttt aaagatggtc atatcattaa agaattaaat aaacaacaga ataatagaca
    11401 tacattttat gagttgatta ttcaagaaat tgcaaaattg gatactaaaa gataggagga
    11461 taatttaatg aacatttttt atacggcaat aaagaatctt aaaaaaaact tttcattcta
    11521 ttctctatat ctactttcag tctcttttgt tattactgta tttttcgcat ttacttcatt
    11581 ttctgttaat aagataatgc tagaaaaaat ttcaactgat ggtagagttg aatctatgtg
    11641 taatgttatt tcgatatttt tgatggcatt tgtcatcttc tatatggcat actcaaatcg
    11701 attcttcttg agacgtcgta caaaagaatt aggtatatat gctttacttg gctatcgtaa
    11761 gtttacaata ttatcgctac ttacctttga aaatgtatta atttgtagtg gagcttttat
    11821 tattggattg gtattaggag cattggcaca taagggtatt gtctttggaa ttacagcact
    11881 acttaattta tcaattgata attcacagat accatttttt aatataaatg caataaataa
    11941 aacaattatc tttatagcat tagttgtttt agtcttagct gtatcaaatg gtaaattttt
    12001 ttataagact ccacttgtaa aattgataag atatgaaaaa agtgcagaaa aaaaattaaa
    12061 gttcagaaaa attccagcct ttatggggtt tattatgatt ttaagcgggt attgtttatc
    12121 actagatatt cttcgtggag ctaaatcagt atgggtaagt attggatttt atcaaatggg
    12181 cttgataact atgattatca ttgtaattgg tacagttctc tttatcaatt cctttcttcc
    12241 atatattatg caaaatagta aaaaaaataa acgtactttc tacacagata tgaagataat
    12301 agctattcca aactttatct atcgtatacg ttcaaactct aaaactctaa ttatgcttac
    12361 attactctct gctactactt tgacagtttc aagtgttatg gcactgtcct tatactaccc
    12421 tattgcatct gtttcgcgta ttgcaccttc agagatagaa tttagagtag aaaaaaataa
    12481 acaaatagaa gatgtaaaac aaattgtcag tcaatatacg tctaatacag atgatattat
    12541 ttttacacag acaaatattt ataaggtaaa atcttcatca aaagaccttc catttgaata
    12601 tgaaattggt acatctaaga ataatttatc caatagtagt tcttctaaag aagttatttc
    12661 aagagaatca ggatttgaat gtatatcttt ttctgattat tatactttat taaaggcaca
    12721 aaatcgaaca aagctactaa aacaacttac ccctttaagg gataatgaat gtattttaat
    12781 gaaatatcaa ccaaataatg atggatatag tgaaaatgga acgatttatc cattacttgt
    12841 taatggtaaa gaaacacctg tagctgtaag ggaaacatca ttaaacaatg ctatttcttt
    12901 tgccaatagc gttggaacac ttatcgttaa tgatagtata tatgagcaaa tagcacaaaa
    12961 taaattacca atgacaagca ttttaagtat taatggtaaa tcaattaaaa ataatgaaaa
    13021 tttatatagt aatatttcag attatttaga tggaagccct tatttacaag gatattctca
    13081 caggataaat gatttgttat caattaatag ttctacattt ctgttaatcg gatttcttgt
    13141 ggtattattc tttattgctg ttggaagtat tttatacttt aacaatatat cagcaatttc
    13201 agattcaaca tctgattata agattttaca gaaaatgggt tatgataata tacaaattaa
    13261 aaaaattatt aaaaaacaag tattgccatt ttttaatatt ccgtttttac ttggtatatt
    13321 ggactgtata tttgctacct tggtttataa gactgctttg atgcagaatt tacttggaaa
    13381 ttcattttca caatacatac ctgtgattat tgcaattttc ttaacttctg tagtgtattt
    13441 gatatattat atacttacta ttaaatcctg ttgtaaaatt gtattaaaaa attaatattt
    13501 accaaatcgt caagcttaga ggtgtgacga tttggtatac ttcatttata agtatttgat
    13561 ataatttaaa attatagatt cttaggtttg atttagtcat aaaattaata taattaactc
    13621 ataaggaggt gtataagatt gacaaatttt aatttaaaaa tactattttt aggaaactac
    13681 attctatcac ttctcttcat gggaatatct acaagcactg aaacttatat gaatatccaa
    13741 agcattattc ccttaataat ttcaacatta ttactcatag acatcacata tataacctct
    13801 tgtaatgtcc gagaaaataa cattatttca ttgttttgtg gattactagc actagacagt
    13861 tggtatttac tcttatcatt tgtaaccaca ccaataggca ctattatttt taaaatatta
    13921 agcacagtta tagtgtatgt ctctattaaa tttatatttt tatttttgtt tcaaggtagt
    13981 ggatacaatt tccgtaagat aacagacatt ttattgctta tcacctgcat tggtgcaatt
    14041 gtaggagcat ttatttctaa cagaacgtat gcttgtatgt acggaataca gtttgttgtt
    14101 agtataataa gctttttcta tattattttt tatcatttga aaagggttgt ttttgtcata
    14161 aaaagcgaaa aaaaacctat atcgatttca gtatttataa caattatagg attccttact
    14221 tattattttt ttacactaag aatatcaaat catattggta attttggaat ctatctaact
    14281 gtactggtat tttttatgag tattcatggt attgcactta aagaaaatga tagttttcct
    14341 atttcaacag tttttaacat aaaacaaagg gctatatttc tttttataag tattttagta
    14401 attagcttgg tcgtactatt tgccaatggg aatttttcta taatattaat agctataaat
    14461 gttttattta tgctcatttt cctctgtaac attattttgg agtttaacct tagagatgaa
    14521 aaaaataaag tagttaaaaa tagcaagtat acttttgcat tagaaaaatt acaacaagaa
    14581 gaacaactaa aaacagagtt ttctaatttt ttacatgatg acatattaca gaacctgctt
    14641 tcaataaaaa acatgatgag taagtcatat cgcccagatg ttcaagatat tatatatgaa
    14701 acacttaaca atctaaatac atgtataagg aagcaaatgc aagactatca tcctgttatt
    14761 ttaaaaaatt taacagtaaa agaaaattat cagaatttaa ttgaaagtgt ttcttcatcc
    14821 tttccacaaa aaaatatttg tgtttcattt aagtgttctg atactttatt cttagttgaa
    14881 ccatatgata tattgatata tcgactgatt aaagaattat tgacaaatgt gtataaacat
    14941 tcaaatggta gccatgcatg gatagcttta gcactagaaa aaaatataat aaaattaaat
    15001 attagtgata atggtgtggc aaatacatct tttaaaacgc ctttagatat aactaaaatt
    15061 acttttgata caacaaaaca caaagggatt atatcaatta aagaacaagt agaaaatcta
    15121 ggaggaacga taagtgtttc tgataatata cctcatggag tttgtgtaca gattacaatc
    15181 cctatgaaag gagatggttc ttataaatat tttgttagtt gatgaccata tgctttttgc
    15241 taaaagcttg tcaattgcac tagaagaata caaagaaatc gaacagtttt attccacaca
    15301 ggacatccat aatctttcag aaatgattca agataaaaaa atagacattg tcttaatgga
    15361 tataaacctt agaggcttaa gtgaatgtga tggcttaaaa gtagcttata atttattaga
    15421 gactaattca actattaaaa taataatctt gactggatac gacctcccag tttataaaca
    15481 tgaggctaaa aaaataggta tctatggatt cctaaataag aatattagcc ctgatagttt
    15541 aattgattca ttattaaaag tctatcaagg gggaacttgt tttccatctg aaaatactga
    15601 agtaattatt gaagatttaa ctactatgga gaaaagaatt ttacagctta tctgtagtgg
    15661 taaaaaaaga aaaataattg ctgatgaatt gtatataagt gaaagaacat tatcaaatca
    15721 tttacaacac atatatgaca agctagatgt ttcatcatca gttgaggcta ttacaaaagc
    15781 tattaaaatg ggttatattt ctccagtata ttaaaaaaag tgagtgtctc aaaataaatc
    15841 ttctaactca taaagcactc tcctttgtat taaatcaaat atattttcat catttaaaca
    15901 attaagaaga ggcttctctc tattttgaga caactccatt ttaatacact atttatacat
    15961 aaacatattt taaacaacta aaaagcaact aatatcttaa taaatatctc aatacatatc
    16021 ttcataacac atatcaccat aattacaagc agataaagaa ctctcctcat ttaaatacga
    16081 ctcaaattca tacccaaaca gaattgttgg ttctatatat atttccatat ctgtatatct
    16141 ccatcttctt acttttgcct ctataacctt gtaaaaatct tctaaactat acccttcttt
    16201 taatctttta tctataagtg ccttagttat actcaaatca gctttaaaat tttttcctga
    16261 ttcattattt aattttctta taataatttc atgtatgctt tctggatttt tgtaagtaga
    16321 atcttcagtc aaatcttcat ccgaattttc aaaggtattt ttatttaaat tctttttatt
    16381 taaattattt tttaataatt cttttttctt tgtctcattt tctgtttcat acccagtatg
    16441 aattatcaaa tcataatctt tgtctatact ctcttgattt atacggctgt atatgttggt
    16501 attactgttc ttaaacaact catctctatg tttatatttc attgtttttt ggttattgtt
    16561 aatagttgca tcaatgtatt tctctatatt tttgaaaata tcaccattct ttgttacaat
    16621 ttctgcatta ctactttcag aacaaacata actatataca gaacaacaat ttccctttac
    16681 tcccctactt attaatctca atatacctaa atctataaat tcatttacaa gtctacttat
    16741 tgtagatata gatacatcaa ggtcagatgc agctttcctt actgtcatca taaattgtcc
    16801 tcttggcaga gttcctttta atctttgtat attttctttt ctcattacat agtgattaaa
    16861 ccttattcta tttacatctc ttttttcatt aaaatataat actgctgaat ttaattgata
    16921 aaaactatta ctcatattcc cactcctcca atttaaatag ataatttgtt gtaaagtttt
    16981 tagattttta aataacttat taaatgatta tttttattaa aaagtattct ttgatatttt
    17041 cattttaaag ttttaagtat aataagttaa tagtgtagat ttaacttttt tatttaaaca
    17101 ataaacttaa tattattttt aatataaaaa ttttattaaa tttagtatgt aattttttaa
    17161 tcttatttta ttccatctat agtcttatgc taaataataa ctttgataat atttccctaa
    17221 cttatgtttc aactggttta tattgcaatt tatttaatta cagcttattt gcataataat
    17281 tcgtccatat cataagttgt gtatttatat aaatacacaa ttaaaaataa atcactatgg
    17341 tatactaatt attacgaaga ctatacttat atgcaaagat aaatataatg catcttatat
    17401 cttttattaa ttataaatat cttttatcaa ttataaaaaa ttaaacgcta tcttatttat
    17461 cataaattac aaaaacataa aattttatta ctatattgct ataaattaat tgatacacat
    17521 tttacacaat aaggagggct atatatgaca aatgctaaaa gtaaaaaaga atatctaaat
    17581 cgtatttgca aagtgcaaga ctatatagaa gaacatttac atgaacctct ttctcttgat
    17641 gaactttcta atatagcagg tttttcaaaa tttcactttc atagaatctt caaagcaata
    17701 gaaaaagaaa ctttggcaca atatgtaaat aggctaaaac tagaaaatgc aactgctttt
    17761 cttattcata gaactgacat gactgttact gatattgcac actactttgg ctttactgat
    17821 tcagcagttt tctcgagagc atttaaaaat tactataaaa taagccctat aaaatataga
    17881 aacaattata gcaagaattg caaagaacca tataaaattt ctcagtacaa tagaagcata
    17941 tcaaaaccta aatgtaagaa tactagagag gttaaaggtg aaattgaaat tctagaactt
    18001 gataatatga atactattta cactagatat actggttcat atggtaatct aaccagtact
    18061 ttcccaaaac ttttagaacg gttattcaca tatgcaagtg agcagaatct acttggactt
    18121 gaaaacacac aaatattagc catttatcat gataatccac aatttacaca ggagcatcaa
    18181 ttaaggacta gtatttgcat gaccattcca gataacatag ttgtcaaaga gaatgacaat
    18241 cttggcaata tgataatacc ttctggaaaa tatcttgtgg gacattttga tatttcaaaa
    18301 tctgaatata gtgatgcgtg ggattttatg tataatgaat ggctatcaaa tggtaattat
    18361 aagctacgtg cctccttccc ttttgaagta tatcttaaca acccacacaa tcaccctcaa
    18421 aatagacact tggtcgatat atatttacct atagaacctt tttaataacc tcacctaatc
    18481 tattcttgta ttaaggatta atatatccct attttacggt ttgataaaat tgataaaatg
    18541 aggtgaacca atgaaagaaa taaaaattaa tggagcaaag atacacaatc ttaaaaatat
    18601 agatgtttct atccccaaag ataagcttgt tgttgcaaca ggtgtcagtg gctcaggtaa
    18661 gtctagcctt atgtttgaca ttgtatttga ggaagggcgt aaacaatatc tgcaatcttt
    18721 aggtatactt gcaggtattg atagtgagga taagtttgat aatattcaag gtttagctcc
    18781 cactatagct gttcaacaaa atattattcg ccagagcaat cctcgttcaa cagtaggcac
    18841 aagaaccaat attctcaaca tgcttacact attgtattca gtggaaggtc aaataatgtg
    18901 tactatgtgt gatactccta taagcgataa tctaatttgc aataattgtg gatatgagga
    18961 agaacgatta agaccaagtt atttctcata caactcttca gatggaatgt gtatgaagtg
    19021 ctctggacat ggtgcatatt ttgagattaa tattgaaaaa ttagtccttg ataagcatga
    19081 caccctaaaa caagtcttag atagagcaaa gattacacca ggatatttga gagtattttc
    19141 taaaaaattc aacgattatt tagacataca attcttacaa ctacctgaag aaataagaaa
    19201 tgaagtactc tatggtcatt atgaaaatgg taaaaaaagt tcctctttat caaaggtatt
    19261 tcacaatcgt tacaaaaggg gcgaagattt aaatggagtt tatactatga ctgcctgttc
    19321 agattgtaat ggttttaaaa ttggtgaaga agcacgtaga gtacttttaa acggaaaaca
    19381 tattggagaa cttggaaaaa tgacgatttt agaaattgat gatttcctta aactcttaat
    19441 aaatcaaggt aatttaaaca catttggtac aaatcttgta aatgatattt tatccaaaac
    19501 tcaacattta atagaatcac gattaggtca tctatcctta taccgagaaa tgtcaacttt
    19561 gagtggtggg gaaatgcaaa gactattttt aaattcacat ctcgattcag agatggactc
    19621 attaatttat gtgcttgatg aaccaacagc tggattgcat gaatcagaaa aaatagacat
    19681 cttaaaatca ataaagaaac ttaaagattt aggcaatacg gtcatagtag ttgagcatga
    19741 taaaaacaca attaaaatgg cagaacatat tattgatatt ggtccaaaag ctggtgttga
    19801 aggtggtcaa ttagtatatc aaggagattt agaaggtcta cttcaatcca acaaatcact
    19861 tactgggcag tatctatctg gaaaatatcc aatgcctaat agaacaagca ataaaaatat
    19921 tacacatact gataaaatac cttgtataac tattcaaaat gcaaatacaa ataatcttaa
    19981 aaatgtaact gtatcattac ctttgagtgc tatggttgga atatctggaa aatcaggtag
    20041 tggcaagagt tcacttatat cagacaccct acttcctctt cttagaagcc aatttaataa
    20101 ccatactatc aataatcaaa taaattcttc agagagtgaa ataggtgagg aagacgatta
    20161 tgcagttgtt gaaactattg cagatagact gattggtact aaacatattt ctggatattc
    20221 agagatatcc caatctccta ttggcaaaaa tatgaactca actcctgctt cttatattgg
    20281 tatatgggat aagatacgta cactatttgc aggtcagcca gaatcgttag aacgaggatt
    20341 tacagctggt catttctcat ttaactccaa aggtgcttgt cctaaatgta gtggtagtgg
    20401 atatgaaaag gtctggttgg gaaataatct aagtatagac catatatgca gtgaatgtca
    20461 gggaaaaaga tttaatgatg aatctttatc tattaaatat aaaaataaaa atatacatga
    20521 tattttaaat atgagtgttt ctgaatctgt atatttcttc aaggatattc caaatattgt
    20581 ttcacactta aatgtattag aacaaatagg tatggggtat ataaaattag gccaacctac
    20641 accaaattta agtggtggtg aatcccaaag aatcaaactt gcaaaagaaa ttggtaaaaa
    20701 acgaaatggt aatatccttt atgtattgga tgagccaact actggtctaa gtctatatga
    20761 tacttctaag ttgattcaat tattagatga attggttgaa aatggaaatt ctgtaattgt
    20821 tgttgaacat gatatagatg tattaaatgt ttgtgactgg attgttgagc ttggtccaga
    20881 aggtggtgac aaaggaggtt atataattgc agaaggttct ccaaaagctc taaaagaaaa
    20941 tccaaaatct ataacaggga ggtatcttta atgaataaaa acaattgctt agacttaatt
    21001 tttgaaaaag tagacccttc aagtgttggt atgagttcag aaaagctttc acaacttgaa
    21061 cctttaataa tctcacaata tagcaatata aatggtataa ttattgcaag aaaaggaaaa
    21121 attgtctttg aaaagtacta taatggatat aatgctgatg atacttttca tgttgcatca
    21181 gttacaaaaa gtattatatc agccttaata ggtattgcaa tagataaagg atatataaaa
    21241 gatgttgacc aaaaggttgt tgattttttt cctgaatata cttgtagtcc ttctgaaata
    21301 tgtaaaaaag cagtaactat acgtcaccta cttacaatga ctgcaccata cccttttaaa
    21361 aatatgactg agtctttaga aagactttgc aaacaaaaag attggataaa atataccttg
    21421 aatcaacttg gtcaaaaagg cgaaattggt actttcaaat attcttcagc tggaacgcac
    21481 ctactttcag ctattataac aaaaactact ggaaaatctg ctcgtgaatt tgctaatgag
    21541 tacttattta aacctattgg tatgaaagtt ataccagatt atgatatgaa agattttggt
    21601 tttgaagatt tctttggaaa aaatcttaag ggttgggcaa aagatagaca gggttattct
    21661 ataggtggct ggggacttac attgagtgct agagatatgt tgcgatttgg ttttctgtat
    21721 ttaaatgatg gtatttggaa taataaacgt attatatcta aatcgtggat tgaagaatca
    21781 gttaaaatga atagtaatga atacggttat ctgtggtggc tatgtaaaaa agatggtgtt
    21841 ttgtggtatt cagcaatggg aactggagga aatgctattt gttgtattcc acaatatgac
    21901 ttagtaatag ccataccatc taaaatagtg tttaaacctc gtgatagatg ggagtttatt
    21961 gaaaaatata tacttacttc tataatagat taattactct ttcataaatt aattactctt
    22021 tcataaacta attactctta gatagccaaa ccctccatta taacaccaga cttttgtttg
    22081 gtgttattat gactaatact atatatctaa aaggagttga atactaatgg acacttacac
    22141 gacatcagag gtagcagatt taattggtgt acatcctaat acagtacgtt tatatgaaga
    22201 actaaactta atacctaaac ctaagcgtaa taccaatggt tatcgtatct ttaatgattt
    22261 tcatatagaa caatttagat ttgctcgtac agccttgaaa attgaagtgg tacaaagggg
    22321 attaagaaaa gatgcccttg agattgtaag aacatcagcg aatagggact ttgataaggc
    22381 tattagtctt gtaaatcaat acttgcaaca attagaaata gaaaaatcta atgccgagga
    22441 agcacttact attgttgcac agattttaaa tggaaatact ccagaaacaa ataaactctt
    22501 tttaactaga aaagaaactg cagaacactt aaacgttaca atagatactt taagaaattg
    22561 ggaaatgaat ggtttgttaa aagtaaaaag aaaacaaaat ggttatcgtg tatatactga
    22621 tgaagatata aaacgactta aaattataag cgcacttcgt tgtgcaaact attctctttc
    22681 agctatttta cgaatgttaa atgctttatc ttctaatcct aatactaata ttaaaactgt
    22741 ccttgacact ccaaaagatg atgacgaaat tatctcagtg tgtgacaaac tgataacttc
    22801 attgaaagaa gctgaacata atgcaaaaac catgaaatct catcttcaaa ttatgaaaaa
    22861 acaattttcc tagccctcca ctctaactcc agactttgat ttgtgtgtta tccttaagag
    22921 caagaaaaat tcaacacaca aatcaaagga ggaataggat ggaaaataaa aatactaatt
    22981 ctacatggaa gaaaaaagtc attctttttt tagcaagtca atatatttcc ttatttggct
    23041 catcacttgt acaaatggca atgatatggt atgtcacact tcagacatca tcaggtgtat
    23101 gggtaacagt tttaactgta tgctccttta tacctcaaat gataatttct ttttttgctg
    23161 gtgtatgggc agactactac aatcgaaagg tactcataat tgtatcagat tcaacaattg
    23221 ctatatctac attagttcta attctattta tgatgtcatc aaatggcaca acaagtgaat
    23281 taatagccat atcagtaata tctgttatac gttcactagg aagtggtgta caaacacctg
    23341 ctgtaaacgc aatgattcct caacttgtac cagaagaaca tttaatgcgt tttaatggta
    23401 taaatagtag tctacagtct atagtacaat ttatagttcc tattgtagct gctgcaatac
    23461 ttggatttgg taataaaata aatcaagttc ttttaattga tgtatttact gcaattattg
    23521 ctattgtatt attaatattc atcaaaattc ctaaacatga tataccaaaa caaactgaaa
    23581 aagtctcatt ttttgcagat atggtatcag ggataaagta ctgtttttca gataagttca
    23641 ttagaaaagt gctttttact tatggagcat tcatgcttct ttgtgttcca tcaggatttc
    23701 ttataggact tcttattgaa cgaacatttg gcaagggtta tatatacctg tctattattg
    23761 aaatgattgg atttttaggt atggttttag gtggattgat tataggagtt tttggtggtt
    23821 ttaaaaatag aaataagacc ttttttctag caatattatc ctatgctatt ttttcaatta
    23881 ttctaggtct tgtaacacaa gcttggcaat tttttatatt tatgtttttt acgaattttt
    23941 caattccaat cattcaagca tcagctatga ctatgttgca agagcatgta gcaccacaaa
    24001 tgctaggtag agttcttagc ttacctatta ttatatatac tggttttatt cctcttggta
    24061 tgatgatttt cggaccaatg gcagatgtaa tttctattaa taatttaata atcatagcag
    24121 ggattatact tttatttttt gctttggcta tcccctattc aaaacaattt tacaagcaag
    24181 gaatatctaa gtcaaatgaa tccgagtaat atatttaaat agtttaactc gatttatctg
    24241 gttaaaaatg aaggggctta aaatggactt tttaatttat gaagaaaatt ttttatatga
    24301 aatcaaatat attttatcat ttcaacattg aaaaataggt ttatctttat tttgagtccc
    24361 tctctttata gcaaataata ttttgatact atctttatta actttatata ataatacctt
    24421 tacttgtagg ttataatgat gaaatttatc aaatcatata ataatacctc catttatatc
    24481 ataaattaaa tttatagtac aaatggagga aaataaagta tttatatagt ttaagtacct
    24541 attaagtttt ccactaagta gctctaattg tactgaactt cttactaaat tatattgaat
    24601 aattataaaa taaaactaac tattacacta cataaattaa agtatacaaa ttgcactaca
    24661 taaattacac tatacaaagt atattaaatc acctattaaa ttgtcccaat tataattata
    24721 ctgagttatc tactaaaaga taactttctt tttttatcta aaataatata tatcataaac
    24781 catactacac aatgtacagc caaaattaca atatctttga taataatgac attaccatta
    24841 ataatctcca taataccatt aaatgtagca actgaaggca ttatatagca taaattccct
    24901 ataccactgg tattaacccc tagaagatat attaacaaag gagtacctat aaataaaatc
    24961 attactattt taatataaac aattcctacc ataagacctt ctgacaactt tccaataaat
    25021 aaacctataa gtgatgaaat aaatgctgaa aatacaacga gtaacaacat cattatcata
    25081 tctaatattg aaatatccaa acatataaat acagttatca ttgaagataa acacccacat
    25141 aaaagaccca aaaatacttt ttgtataata tactgacttc tactctgtgg caaaacttca
    25201 tttataaatg atattccatc ttctttttcc gatataatat tcattgcatt aaaggtgcat
    25261 cccataaaca tagcagtaat taaaatcata gcaataaaaa cattttgaaa cccttttaaa
    25321 atactatatt taggcaatat tgtaacctca acatctttta aatcaccctt tagtctaaac
    25381 atctctggca atgtctttcc aacatttttt atagcattaa tctcatttcc ataaagcaca
    25441 gtttttatcc ctttaccatc tctaacaact ccaattgtgt ccgttttaat atccttaatt
    25501 tcacgtatta aatgcatttt tgtgtcatat acacttacca taccatactt cttcaaccac
    25561 tgctctgttt gtacaggaag tgtatccttc actataccaa aacgtatctc tcctaatgaa
    25621 gatatatcaa ttgttcccat aaaatttatt aaaatcgcca taacaactgg taaaaagaag
    25681 gaaattatac acattttatc tttttttata ctctttaact ggtaacgtaa tcctatcaag
    25741 attatccctc cctaaccaac tctttattaa aaatatattt tacaagtaaa aatagaacta
    25801 ttattgtcaa agaacacact acaaaataaa ctaattcttt tgaaaaattc atgaaaaacg
    25861 catttttaag ctgactataa acatgataga agggatgata tttaatccaa tctatttcaa
    25921 aagatgtatt tgcagataga aatataggtg tagttatgaa tacagcaacc acaagatata
    25981 taagagaaaa ttgcttaaaa tctctcaata acattgagca tcctattcca atcaaagaca
    26041 taataattga taaaatacta gcttgaacta atacttgtgg aagtattttt attccttcta
    26101 aaaatccaat attaaatact actaaaagaa ctgtaaaaca taggtcacat ataaaaaaag
    26161 caaatatctt tgataatata aaaagacttc tttttacagg taaaatacca gtaatacgca
    26221 ttactcccat ctgcttttct ttaaataata aagaggcaat ccctaaaaat ccaatagcta
    26281 caatttcaaa gaataaaatc tcacaagtca tgtcttttct ttgttttatc tcagatgtat
    26341 ccttatcatt aatttgaatt attgcattgt tgtctgatat tttgctatta tggtcttttt
    26401 ctgatgataa taatgacaat gcatagtcag ctctataatt gtcaactcct ttaatacttt
    26461 catgtaaaat tactttcggc ttactggaac tcatatcaat acaaactgca cttgtatctt
    26521 catcaagttt tgcttttaat tcacctatag aagaaacttg attaattaat ggagatgcac
    26581 tcatgcttat attctctgga ttatatagat atacattatt gtgataacta tcttcatcca
    26641 taaactttac ataaccaaaa ttaatataac acgaataaag tatgagagag ccaagtgtca
    26701 gcacaaaaaa tttaccataa atcatcatcc aaaaatcttt ttttactaag gtaaaaaatt
    26761 tagaatacat tacactaaca tccttcctgt gtatttaata aaaacttctt ctagtgtagg
    26821 ttcttttgaa tgtacactta agatttcatc atatttaaaa gttattccag attctaactc
    26881 ttgtatatct acaatttcaa tatctcgatt tccattatat acaaattcca cttcaacttt
    26941 attattgcta tttttcattt ttaaattttt gggtttatct attaccataa tctttccatc
    27001 tgcaataaaa gccacattgt tacacattaa atctgcatct agcatattat gagtagtcaa
    27061 aaagatagtt gttccttttt ttcgttcttc ttcaataatc ttacgaaata gtacagctcc
    27121 agatgggtca agtccactag taggctcatc taaaaataac aactttggat tacttaccaa
    27181 tgctcttgcc atgctaatac gttgcttcat accttttgag taggatgata caggcttatt
    27241 tataaactct ttcttaaact ctaacaaatc taagagttca tatatgtctc tcctttgatt
    27301 tttaggataa aaagacgaaa aatattctaa attatcaatt gcacttaaat tggtatataa
    27361 ataaggaaat tcaaataaaa caccaatctt ttgatataga gactctgtat atgctttaac
    27421 ttccttccca aaaaatggat acgtgcccat tgtagccttt taaaacgcca tttaagattt
    27481 tctgtgttgt ggattttcct gaaccatttg aaccattgac gtatagacaa gtttgctgaa
    27541 tagcaaataa agattaacta aaatgatata attaactaat tattgtattt tagtttttta
    27601 gaaaagtggt tgtcctttat aaattccttc aacctcttat attgatctgc aaatttaaat
    27661 tgaatagtaa tttcattatt ttcatgaata taaatagcat ctatcaattc tattaatatt
    27721 cctctattaa gttcttttat atttttatac ttcaaaaatg tttttagata tggatcatta
    27781 tctccaactc cttttgaaga tgattgtatt tcagcttcta tattagatat aatacgtcta
    27841 atttcctcag ctttaatttc aaacctcgat ttcattctag catactcatt tctactaatt
    27901 tcttcacttt tccaatccat atatagatta tctgtaacac aaataatttt ttctaattct
    27961 ttttgttttt cattaagttg tttctttaat cttgatgact gattgacaat tatggcttgc
    28021 ttattaattt cagaaataac ttcgctcata ttttcaacca tagctatctg tatctgaatt
    28081 gcctttaaaa ctgcttcttc caatatctct agtttaatgg aatgtctact acatttagat
    28141 ttatttttca atgcatatgt agaacatata taataattat attctttaac agttccgtca
    28201 tttaacttat ttttatttgt tttctttgtc atacttcttt tacagtcagc acatcttaaa
    28261 aatccagaaa acaagtatac atttcttcct tttggagaca ttcttatatc tctttgatct
    28321 aattcttgag cctttaagta agtatctttg tcgataatag cttcatgtgt attttctaca
    28381 atgaaccatt cattttctgg agtgcaaata gttttatgaa ctttataact tataactctt
    28441 tgtcgtcctt gtaccatatc tcctatatac attcgatttt ttaatatagt agatattgta
    28501 ttcatagacc acataccatc ctgtttatct agctttggat tttttaacat caaaccttta
    28561 ctatgtttat atttggtagg tgttaatgtt ccaagttcat ttagacgtct tacaattcct
    28621 aatttattta tcccctcttc tactctccaa cgaaaaatat ctcttataac ttgagcagct
    28681 tcctcgtcaa taataagttt attcttatta tctggtgact ttttataccc atatggagca
    28741 aaagccccta taaattctcc attacgacgt tttgtattaa atgttcgtct tatatcagca
    28801 gaagctcttg cagcatacct gtcattcatt aatcctgtaa taggaacatc taatccatca
    28861 gtaattactt ctgggtttaa ataagtatca aatttaggac ttcccaagca aatgaatctt
    28921 gtcctatgca atggtaaaaa ttcttctaga aaatatcctt gatctgaata atttctaaat
    28981 gccctggcta atgtcttaca aataatacaa tttacttttc cttgctttac atctttaatc
    29041 atacgctgaa aactttcacg ctcatcatca gttcctgtta atccatcatc aacataaaaa
    29101 tcaactaaaa catattcatc ttcaaacatt tcctctaaat attctttaag tatctttttc
    29161 tggtttataa cgcttaaact ttcatcattt ccatcttcac gagaaagtct aatataaaca
    29221 gcaactttcc atatagtcct gttatttgtc aatcttgact cctgtgattt tcttctacgt
    29281 ggcatatttc ctccaatctt ctataatcac aacaaaataa taacatattt cacaacagtt
    29341 ttcagcagtt tttgaggttt ttaaaatagc ttatcaaaca taaatctagt gtattttcac
    29401 tatctgaaaa ttttaacctt acaacaacat ctccgcatat aaaacaatat ggacttttca
    29461 tctcttttaa acatctttct aaatcttcat cctctaaatc tttttgttta ttgataactt
    29521 tatcaatatc tatatacttt ttatttaatt tttcattatc tttttttact atatttacac
    29581 tcataaattc tcccccaata atattacttc aattagtaag ataactttat ttaaagccat
    29641 caattagtta actctgcatg tttctcattt atatccatca tctaactaat agcattaatg
    29701 atataccaaa atataatata ttaggtcatg taaaagattt aataattttt ttaacttatt
    29761 gttatagata actaatagaa tactctatat ttaactaaac aattatacgg ctctggcaag
    29821 ctccacagga atttcacctt atcccattct tatgggtata ccgttgtttc aaacgggagt
    29881 atcattatac taatatataa atcatggcag ccattttgcc aaaatgactt acagatcact
    29941 aactttaatc gctctctttt ttaataaaag gtcttggcga attagtctgt cggctcatta
    30001 tatattaagt gtatataatt gttttattca cttttcaaag atccctctag acctattcat
    30061 tgaatatcgg cctattaaaa tatattagaa tttaatatac tttaataaac tgatattttt
    30121 ctcagctaga gaagtacaaa ataggattaa aatactcctc tagccaaatt tatcttatac
    30181 gaatttaaat tttaaaattt tataaataag ctttaattca agtaaattct ttttttctaa
    30241 atctaaacat ttataagcat ttcctcgctt atcatataga gtctttgtgc aaagtttaac
    30301 aatgtaggct tcataatttt ttaaaactat tcctatagca attatgtcac cctttgcagc
    30361 tgcataaata atatcgtatg gtaataattt attattctta tgtaccaaac tcatttttct
    30421 ttacccccaa tatttttctt aatgttttga ttgcaacatt tctttgatac cacacagtat
    30481 cttgactaac atttaaaatg cctgaaattt ccttatcgtt gtacccaata aaatatgaaa
    30541 gcaaaattat tttttgtctt aattctggta ataatttaat agcttctcct aaagcttcat
    30601 ttactatatg aattttaaat ccatttacat taaagctata ataatcactc aaatatagat
    30661 catttgaaaa aaggctattc ttttgttctt ctgataattc gcaaaataaa atttgtacct
    30721 gcctaagata tctattcttt ctctcaatat caatagattc attttttaat atttttttac
    30781 aaaagctatc aaacttatgt atcgtggatt ctttatttat tgaactattt ttcattttct
    30841 cgcctcctct cgcattaagc taaagtggtg ataaatatcc tttttcttcc ccccttctac
    30901 tattaccacg aatgagagac tcgtaattcc gtaaaatata tacaaaaagc acataattag
    30961 tcatagaact aactacatgc ttaaaaactt tcgtatatct tttataaacc aaaaatctta
    31021 tttatttcct ttgtattaaa tctagcataa ccttttaatg caacaaatgc gataaaaata
    31081 cttctcaaat atattcatag ttataaattt aaaaattaaa aaattcctaa aaataaaaag
    31141 atgagtacta taatactcat ctttttattt tttaattttg tgtgagtttt agttggtaat
    31201 agtcattaac cttaaagaaa ttttagtttg tatgcgttat gactataata tttagggtat
    31261 gttttattta tatcacattt agccaaatta tatctgtcta tatttttgta taatatgaat
    31321 atatttgaaa cttttatatt ttaaacctat ctttaactag aaattatata ttattaaata
    31381 ttttcaagat ttttatcatt ttatataata tattcattta actatatcgt ataaagatag
    31441 aaaaaatata taataaaaat gcgagtagca atattactac tcgcattttt gttatattgt
    31501 cttaaaatat atagattttg tgaccatgaa ttttatttaa taagacaatt taacaaataa
    31561 agaggtatgg ttaagtatta aataaacatt gtatggattg tttatagtac ataacgtact
    31621 ttacttatat catatctaca atttaaataa ctctcatatt tttttaaaat ataaattttt
    31681 ttgagtttat cattttatag gcttaactta tttttgttgt ttacaatatc ttcaaatgtc
    31741 taaatactgt tttatctata ttatcaatat ttaactctgt aaactatgat atatatctaa
    31801 tacatccatt agcttatgtt acattcacca ttttttctta gcatcttatt ttttcactta
    31861 tgattacttc tattaattta aatatgtcaa tcttctgatc acaacttcta tataaacaat
    31921 tattaaataa attaatatct taattgctca caattatgta taataacttc taaaaagctt
    31981 tactttcatt tattgatttt tttagttatg gcaatatatt gatatagtct ataagaggat
    32041 gtagtttacc atcattatac attttaacta attcaccacg atcaacccat tttacatcta
    32101 taacttcttt atcctgtaaa caaatctcct caatatctaa atccttaaag aataaccata
    32161 catcataaaa atctttggtt ttagttcgac aagtttgata aattattttt cctcctaatg
    32221 gatttaaata aatacctagt tcctccttaa cttcacgcac agctcctgct aaactgtctt
    32281 cgcctgaaat aattgaacct cctgtacatt cccaatacaa aggatatgtc ttttgagaat
    32341 gtctctgaga gatgagatac tgtcctttgc tatttctaat ccaaacactt acagaaatat
    32401 gaaaaagtcc atttggaata ggactatttc tacttattac attgcctgtt ctctttcttt
    32461 ccccatcata taaatcccat agttctgtca taattacact cctttatatg gcaatggtgc
    32521 cgaattaaaa tctaatatgt acatatcagc aatacttttt atttcatttt gatttttttc
    32581 tgagtactta tcatatactc cgcaaacttg cattccaatc ttttttgcac tttttatagc
    32641 tggaagaata tcatcaaata caatacattt ttcaggatca actccagttt ttactgaaac
    32701 aagtctaaat atatctggat gttcctttct gtaagatact tcatctgtag aacataaaac
    32761 atcaaatagc tccaatacat tattattttt aagacatgga aaatatagct ctttagaaag
    32821 tcctgttgct atggccaact ttactcccat tgattttaat ttaataatat attccattgc
    32881 aggctcaaaa atcttgacat tattgctata ttccacgaga gcactttcat tccactcctc
    32941 aataatatct tctactgctt catttaagtt gaacaaatca atagtatatt gtgcagcttc
    33001 tataaggctc ctatgacata tgtcattcat ataactatca ggaacaacta aattcctttt
    33061 ttctaaaaat gaattattta tacgctccca cgcatacata gagtcaagta atgtgccatc
    33121 taaatcaaat atcgcaactt caaaatacat tttaatacat ctctccttct agctataaaa
    33181 ttttactttt tattgttgac taatatttaa gcataaattt acatatcctg tgaaattgac
    33241 taaacttcaa gatttctgga attactttcg acatattaac gttatatctc tacatgcaat
    33301 aactctccct atcatcgctc caaaatttaa attaaataat acttcagaag atatagaatc
    33361 taaatattca gttagctcat ctgcaagaat tatatctcca agcttattgt ccttgctaat
    33421 gctactcttt attgctaaaa aatattctta tatattatca tgtcattaat taaagcaaat
    33481 gattgaaata agtaattaat tgtattacgc cttaaaattg ttgttgctct actttctatt
    33541 ttagaaaatt cttttccatt tatcttaatc tttccttcat cactttcttc taaaagttct
    33601 atcatattta gaagagttga ttcttcacac ccactttgtc taacaattgc aataaactct
    33661 ccatattctt aaaaaaaata accatctaaa taactgctaa tgtacccatt actatagctc
    33721 caaatttaaa acctataata agtataataa ttaattctat attttcttct acactgatat
    33781 ttctaaattt tatatatcca ttaaatatct ttaagctatt tgaattttta ttataatata
    33841 ttaaatagta aaaatgcgaa tagtaatatt actactcgca cttttgttat cctgtcttaa
    33901 aatatacctt taattttata taataagaca atttgacaaa taaagaggta caattgtatg
    33961 gattgtttat agtacataac atgctttatt tatatcatat ctacaattca aatatttatc
    34021 atatttttct ttaaatataa aaacatttga actgttcatt ttttctatat accttactta
    34081 ttttttaata atatcttaaa atttctaaat actatttcat ctatattatc aacatttaat
    34141 tctttcccta ttaactcata aatcaatgaa atattattgg ctttactttc ttttttactt
    34201 tcatagacca ttatatcctt aagctctttc atggatatta tacatgcttt ttatagtttt
    34261 tccatttctt aattagttaa acttacagcc ttaatagcaa gctttcttat atcatcttaa
    34321 tttaataata aatacatact cgaactctat agtaaagctt ttaaatcact agacctacac
    34381 tatatttttt tgtatttatt ttaaattact attgactttt ttgttgaaaa tgattatcat
    34441 tataaataac taaataagta tattttgcca attttttaat atgtaatcct taactaattt
    34501 aagaactaca tattcccaga gagaagaaga tttgacctcc ttaaacttca aatcttcttt
    34561 tcttatgcca aaaataaata attttcattt ataatatgtt tatctgtgct cccctaatta
    34621 ttaaacacca cagccaaatt ccttttacaa tttgtttcta ataatttttc aaaattaata
    34681 acttttctca ttctttctta tttaattatt tatatttata aaaaaataaa cccctaaaaa
    34741 ttagggattt attttaaaaa cagcattaaa attttattat ttttgacttg gaagtaatcg
    34801 atgtataaag atattaaatt tgacttttct tattatatta tactaataac tttaaaaata
    34861 tttatgtcta tttttgttct aaattaataa tataatgaga ttttttctat ttaacctaat
    34921 tctatataga tatagtaata tattctaaaa atattaaata cagtattaaa acttaaatta
    34981 ttcatctaat tctatcatta gataaatata caaagtatct atttatttaa acagcaaaaa
    35041 tgagtattga ttccaatact catttttatt gtttaatctt ataggtcgtg aattaaatga
    35101 acatatagct agttaattaa aaactaagcc aatattccta agcgtcagcc ggaatatctt
    35161 tagttacatt atgtaaccta ctttagttat aacatatcta gactaaaata ttattccttt
    35221 ttttcacaaa aacataaaaa tatttggagt aactatattt ttttgtttca caacttattt
    35281 caaaatagaa atttatgatt tttaaacttt ttcattttta catatttaat aaaaatatac
    35341 gaaaacaata aaaagatgag tagtagtact acccatcttt ttaccacttg accttaaagt
    35401 atttaaactt tatggccatt atttttttct taaggggact aattaaataa ggagttataa
    35461 attaagtatt ccataaatac aatatggata aattaattaa ggcatataac ccactttatt
    35521 tatatcatat atatgtaaat tttatttatc atacttttat ctaaacttaa atttttttga
    35581 gtgtctttta acagtttcta tagtagatat tctaaattat ttaattgttt tatcatataa
    35641 atatagctct tattaaaatc tatacatgta gttacactag aagctaatct ttattaaaaa
    35701 tcaatctcta attttaagag tttattctag tattttaatt tctcctatat tattaacttt
    35761 ttctttacag ctattattat ttttaaagta tttatcccat gtatctttac ctagtgataa
    35821 tgagtataac tctaatactt caacccaaaa accttcttta tcttctgttg atagtctaag
    35881 tttaccattc ttattttcta cattatacct tttcattgaa tacttattat tattattttt
    35941 tttatcttta aacattgaat tataatcatc agaatctgat aaatataatc ctttaagatt
    36001 ttcattagaa tcataatcag cccccccata cagtaactat atgagctata ttacttccca
    36061 tcatatgaga aattgctaat acacgtcctt ctcctagtct ctttttaact aacttactta
    36121 aatcttcata gttatttatt ttatcacgtt ctgttagtat ttcttcctta aatacgtctc
    36181 taaaaaatcc tcctctatga tcctttttag atgtgctaaa tttgttttta tcatttcttt
    36241 gacctctgtt ttcacctgta agtgatggta tataaccatt aataaacata tcaatagtcg
    36301 tgtcagccca tattcccctt ctccatcttc caaaatattc cttaaataag ttaaaaattt
    36361 tactactatg cttttcttta tcatatgaat ttaataagtc tcttatatct cccttttttc
    36421 tccattaaat tccaatatac catttttagg attttctttt ataaacttat atacataatc
    36481 ttgattttgt tcaagccacc agtgaagcat gttagaggct gttgctccac cacaaagggc
    36541 aaaatcatct atactcagat tacctgttga tttatttgca tcataccacc cattaccttt
    36601 atcttgtgaa gttacataat atttttttgt accaactatc tctttagtaa aatcaccatt
    36661 ttcacccatc cttggaggta tgattccttt tacccataag gtttttatat tctcttcttt
    36721 ttcattttta tagatatatg aattatctat ttcattcgtt aactttgttg ctacttctgg
    36781 tttaactttc tcagaaagtt catatgattt ctttgctccc aacccatatc cttctattgt
    36841 attaccttct ttatcttcta atctccaacc tacaatacct atttctcctg attttgtttc
    36901 aatgacctgt ttatcaaatg ttcttagctc ctctgcataa acagttaata aaccaaaaga
    36961 gagagatact cccgttgcca tcacagttgt tgttattgtt tttgctaaat tgtttttaat
    37021 caatctttta ctccttgaca tgtttatata acatcctttc cctttctact tcttttttta
    37081 tataaaatat gtattttttt tatattatta tatgttttta taatgttata tttttttcga
    37141 aatttatata aaaaagtaaa aaagttaagt atagtttgat cctttttcaa ttcaaactca
    37201 tgcttaaatt catttttata tgtttatata tcacaattga ttttaatcat attttggcat
    37261 gtaaaaagcc cccaactgtt gaattttttc tcaaacaatt tggggcaagt ataattagta
    37321 ttaattttta cttttattta taataaactt tttaaaattc ataatttatt tttttctaaa
    37381 ttttattttg tttaaaacaa ggccaagtaa ccctgaaaat cctaaggctg caaatcctaa
    37441 tgttcctgaa tctcctgttt tagggtttaa attgtctttt atttcttgtt ttttgtataa
    37501 tttaaccgga tcattagctt cttgagtatg atctttcttc ttattacttt caacgatctt
    37561 atcttcaggc tgtttcattt ctggtttctt gtcttgctct ggtttactac tttcaggctg
    37621 tttcatttct ggtttcttgt cttgctctgg tttactactt tcaggctgtt tcatttctgg
    37681 tttcttgtct tgctctggtt tctttacttc aggctgctta tcttcttcct taataccttc
    37741 ccaccttaag tttcctcttt ctcttgataa taaatcaagt aatttattct tctgagattc
    37801 tagctcgtca tttgttgtcc tcaataaaat gctctccata tactgtttat caaactcgtc
    37861 aatgttacct ggtataggct ttttaagtat atctactaat tcttctttgt tctttgattt
    37921 tcttatcttg tccataactt ctgcacgttc tttcccaata gagctctgat ctttttctaa
    37981 attaccattt tttagtttca tataaagtaa tctagccttc tctacatacc cttcatcaaa
    38041 tattgagttg tatttttctt taatttcacg tatattttta taaatttctt caaattcatc
    38101 ttttgcaatc aatgtatcat ttttcccatt aagttctcta ataaatcctt taagtttatt
    38161 taactctaga actttttttg aaatatccct tacttctctt aataattctt tattttcttc
    38221 tgtttccgta tttatgttta taactttttc tatctttttt aattcttttt ctaaaaaatt
    38281 actaactact gtaacatcat attctttttt tacacactct gacatagatc caaccgtaag
    38341 tatcttttga taggaatcag catttttgat aatctcttca atcgctccaa actcagcagc
    38401 tataccactt ttatctagcc ccaaaacact tccatactta tccagtagtg aatctacttc
    38461 actatgtaat ctagcattat cactcagtgc cttatctttt atatccttta aaggtttctc
    38521 aattgctatt tcactttctt ctagttcact atgatctggt ttcttatctt caggctgctt
    38581 accttgttct gatttcttac ctgtttgttc tctatcgtat ttgtcacatg cctctttaaa
    38641 cttatcatat tcttcattaa taaaatttaa aattctataa taggcatttt catcgacatc
    38701 tggtttttta actaaatctt taaaatttga gcaatcatta ttcgctttaa ataaaccaat
    38761 atatatctca atattgctct tttgtctttc taaacctatg cttaattctt tatttaattt
    38821 gcctttatat ttatctaatt tatctttaat ctcttgtaat tttttcaaat caaaaatttc
    38881 aaatatatat tccttattcg attcattttg agttttaatc ttatcttttt caccttcagt
    38941 gttaactttt aattgttcac taaatttatc tgttttcttt gcttctgatc catgcccttc
    39001 tactactttt ccattttcat cttctaagcc ccagcctgta atacctgttt ctgggtattt
    39061 tttatctact ttttttgctt ctgatccatg gccttctatt acttttccat tttcatcttc
    39121 taaaccccaa cctgtaatac ctgtctcagg gtatttttta tctacttttt tcgcttctga
    39181 tccatgacct tctattactt ttccattttc atcttctaaa ccccaacctg taatacctgt
    39241 ttcaggatat ttttcatcta ttttttttgc ttcaatttgt ttatcaactg attttatctc
    39301 ttctgcataa acatttgtca aactaaaagg agcacatact ccagttgcta ttacagttgt
    39361 tgttattgtt ttagttaaat tatttttaat taaccttttt ctgttatttt ttaagttttt
    39421 ccttgatcta gtcatataat ttcacccttt tctaataaat ttccttacat acggattaca
    39481 catattactt tcaagtatta ttatatattt tcaaaattct attttttcac caaaatttgt
    39541 gttaaaaaaa taaaaaaatg acaaaatcaa agaagtacta aaatgcattt tttaaatcta
    39601 caaggtaata atatactata taaacattga aaacttaata ttattttttt atttaaaatc
    39661 tattttttta tataaagaat agacattgaa atttgcattc aatgtctatt ccttgtgtaa
    39721 taacttttta attgtattct ttcctattaa atttttattt ttgaaattat ctttatctca
    39781 taatactatt ttttaattaa ggcttgaaag ttaaaatttt ggttataagt tttaactcta
    39841 tcctactctt cttgttttca tccaaataaa taaaggttct accttgttta tcacatactg
    39901 ttcttgtaca caactttgtt atatacattt catagtgttt taggactact tctattgcac
    39961 ttatatcccc ttttgatgcc tctaaaataa cgctataggg aagtagtcta taatttttat
    40021 tttccacttt aatttacctc caagttattt ttagacttca atttttatat tttttatcta
    40081 taaaatcatc tatttataat tagtataata aatttcccct actacacaat actatatgta
    40141 attttgattt acttttatgt aaaattaact tataactaaa ttattatatt aaattcacta
    40201 tattattagc ctttatagta aataaaaaga gaactcattt tatgagttct ccttgacaag
    40261 tttcattcta atgttaatta aatttcttta atcggaatca ttattaaaac actaaatatg
    40321 ttcttttcat cattatattc ccattctata tctccatcat atttttctat tattgactcc
    40381 attatcctaa caccatatcc atgatactct tttcttcctt ttgttgtaat tagctttcct
    40441 cctttaacaa taggttcttt ctcgcaagtg ttgatcatct ttataattac ataccccttg
    40501 ttaaaactat atagtataaa ctctattttt ctttcagaac atttttctgc actttcaatt
    40561 gcattattaa acaaattact taaaatccct actaagtctt tattttctat aaaatctaaa
    40621 tctatttttt caatattatg agtaaatttt atgttctttt ttttcattag ttctatcttt
    40681 tcatttagca caatatctac aatcttattt tcagtgataa ctttatttcc atacttcttt
    40741 atatctttgc ctacactata taggtattct attagtttat tgttgccatc ttcttttgct
    40801 aatgtatata tagttattaa atgattttta atatcgtgaa ttattttgtt agcatcatca
    40861 ttcttatcag aaagtatatc atagtgagat tttagcaact tatttttttc tttaattatc
    40921 atcttttttc ttatttcatt ataattgaaa attattccgc cagtaataat agcataaata
    40981 aacaatagta taatgttcat taccctaaag aaaaattgtt ttttaaattt ctccgtatat
    41041 tcttgattat attttttatt ataattttgt aagtcatcta aatgaactcc agtcgctatt
    41101 acccaattat actttgggta aagcttagca taacttaatt tcaaactctc atcatttgag
    41161 ttaactttct taaaattata ttcattgtaa gattctcctt ttgcttttat atcttcaagt
    41221 tcttttaagt aaggcatttt tcctttagaa tctttcatat ttgttgataa aaattcacct
    41281 tctctatcta ttagatttgg atgaactaac cttttagcat aattatctcc accattataa
    41341 ttgagtattt cgttaaccca tatgtaccct ccttcatcta atttcgtatc tcttatcttt
    41401 ttaatagcaa tctgctttac cctatcatct atatcgcttt gacacactcc taaaattaat
    41461 agtttagaat tgcctatgtt tttaaccttt cttaatatat aattatcttt cgtatatttt
    41521 aaaatatctt ctttatttat atctttgtta tcattaatat ttttatcatt agaatattct
    41581 atactttttc tagctccatt ccatataatt aattttactt ttgaattttc atcaaattta
    41641 tttcctactt tttgaactag attaactatc ttctctgtct ctagatcttg gctaaaacta
    41701 tcaaaaagaa gtgaaatctc ctcaatctcc ttctccttag tattgataac ccttttttct
    41761 tctaattcaa tatcatttat tgttctatct acaatctctt taatcctttt cttttgtgta
    41821 tcaataacaa tctcattagt gtcctttagt ctctcttgat actctttttt attatcaata
    41881 aaatatttat acattaaaac tcctattgag cttatgcata ttaatacaat agcaatatat
    41941 atattgcgtt tttttctcat attactcata tctcaaacct catattataa tatatctaat
    42001 taacgaattt catttatata ttttataaac ttgtccttaa actccttata tttacgttgg
    42061 gagacatgta ctgattctcc atcagttaat actacgtgat ttttattgac ttgcttaata
    42121 tatttaaagt taactaaata gctactatgt ggtttagcaa aatcatatcc ataaagctta
    42181 ctttcccatt cccctaagga aatcgatgta gaatatactc cattaactgt atatactact
    42241 acttttctat tttctatacc taggtattta atatcactaa ttaatacctt ttctatagct
    42301 ccatctttct ttaataatat aaagcgttta tttttgttaa tttcatttat taaatctttt
    42361 attatactgt ataactcatc ctcttttata ggcttaagaa tatacctata tgcacttact
    42421 gtatatcctt ctggccaata ttgttctagt gctgttataa atataatttt tattttttcg
    42481 ctactctttc ttataatttt agatgtttct atacctgtta gttttttcat ttttatatct
    42541 aaaaaaacta tatcatattt ttcaaactta ttttcaataa gctcttctcc tgaagaaaag
    42601 gtatcaacat ttaacttaat accttcattg agttcaaatt tttttaatat ttcacatatt
    42661 ttttcaactt gaaccttttc atcctcacat atagcaattt tacacatact catcttcctt
    42721 taccttaaaa tcctatagct cataattatc aaaatataat ataattagca aaccattatt
    42781 ttcactaata aacagtttat aaactcacta aaaaatctat ataaatatag attgtataag
    42841 ctttcaattt cacgtttttt attttgcttt atctttataa aaaataaatt taactttaaa
    42901 atttattttt ttataattat ataataccac tcactcttat caatttgttc taatattttc
    42961 ataaaataac aataaatttg atacattaaa tcataaattc cctataaaaa ctaaaaagga
    43021 actaagttta aaaattcaac ctagctcccc tttacttttt acaaatctat ctttttaaaa
    43081 gtgatttagt aaaactaatt atatattttt ttaataaaaa agcaattacc attaaaatac
    43141 taaatatata tattataata ttttcatctc ctgtagctgg agattctttc aatttattta
    43201 ttgaagtatt ttgtttagat aaattgtctt ttttatcttc atttttatta ttaaccacac
    43261 tattactatc tttgcttttt tcttgcttat ttttatctat ttcaacaatc ccgttacaac
    43321 cattttcttc tgtttctact attgttcctt cttt
//
LOCUS       DAMJYC010000027        40068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJYC010000027 DAMJYC010000000
VERSION     DAMJYC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.028"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(326..1294)
                     /locus_tag="PC472_001582"
                     /pseudo
     CDS             complement(326..1294)
                     /locus_tag="PC472_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1294..3513)
                     /locus_tag="PC472_001583"
     CDS             complement(1294..3513)
                     /locus_tag="PC472_001583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726910.1"
                     /translation="MRDRIYIVDINTKMYQIARYKQNDNNVSYNMRIVQDSIDVDLTG
                     YTALAFFNLPSGRVTQKNCTIEDSTVYTELSHIELSEEGDVISEITLYKNDKVVTTFS
                     TIIKVEKSINRNAIEDEPSWDIIKDILNVLSYEEERQENENVRKSNEEIRISSENVRI
                     GNENVRIESENQRKDSEVERCESEEVRKTQEVTREANEETRKTSETTREANEEIRKTS
                     EITREANEEIRKTNEVDRESREETRNTNEETREENETVRESNEEIRKTSEETRESNEN
                     TRKASEEIRDGSEETRVVNEVARQESETTREENEDLRKSNEETREENEEIRKSNENTR
                     KASEEVRNTIEETRNTNEEARKASEEIRKTSETTRNTNEEARKVSETTRETNEETRKT
                     NEIIRQNSETKREENEELRESNEGTRELNESTRQTSIINMQKKVDDKVDEVDNKIVEV
                     NTAKTDMTTTVSNKITEFEKRFNELENLDTAGEILQSKETTDGQIKDTLKERLTYDFN
                     KLDLKIEQLIAGGINVAFNKSYDTSEWIEVDGGFELSVEHNLVTEKILVSLIDKLNNK
                     SLITSYKIIDDNNIVLFNEVAIDIEVTVVNGGSAIELIKFTINDDIESLESAYSSIET
                     KRLLKDGLDKKANLEHTHDIYVEKVEGKELSTEDYTTEEKEKLSQLDTYKNKETKFNT
                     NGSIIEILDNNIKYITKFNTNGSISKEKYINDILINTTITNFKNGMIEETVTNEGVI"
     gene            complement(3520..6288)
                     /locus_tag="PC472_001584"
     CDS             complement(3520..6288)
                     /locus_tag="PC472_001584"
                     /inference="COORDINATES: protein motif:HMM:TIGR01665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2726911.1"
                     /translation="MLRLYKANETNFKNNGLGILKDAFETKVTEELNGLFELEFTYYV
                     GSFLFDEIDYNKIVMADASPRLKNQLFRIYYISKELDGKILVKAEHISYDLLNNFIEN
                     LELKNVTCEEALNQIFRSCTEENRFVGYSDITGNKDFSISCVSPHNAISDIQELFNNK
                     SKLKRDNFNISLLNNIGESNNVLLAYRKNIIGLTATYDTQEVITKIYPYATKKSGKNK
                     KITLPEKYIESKYINNYPTQRIVAVDFSDDDVKNEESLRNKCKDYFIENNVDLPKVTY
                     KVEFVDLSTTEDYKSYKMLETVNMDDEIIVRDYNLGINATARVVKTEYNPVLKKYNSV
                     EIGDLVNHFKDERIDDLEEKIDKVQNNVDNIVIESDNFPDTLPEPSNVTALGLWSMIQ
                     LDWTFDNKLYYNYEVYASQIKGFEPDTIGYTNRIYVGQASSLLHEVKPMQTWYYRVRA
                     GNTHDNYNEFSNEVSATTRKLSDAAEYFEEAAIGHAVIRDLDADKINVGKVKGQYIEA
                     KNLVVVDGNSQTTLNIDSFGNVHIGATTFTLKGKSLESIIGGEIDDITQLEIFNKLTN
                     NGLAKGLYMVGNELYLNASYIKTGTLEGQFINGRNLTVRDNDGYTTLQVDSNGKVNIR
                     ANELSIGDKNNYESVLTSDQKAVFDALTGNRNCGIYLSGSRLYINADYIDTGTILCDR
                     IGASSSNPFILLFEGNGAKCALDATAQFGVGIGKAMRMKYNDYSYIYVSDDVISGYLD
                     GEEVFEFGYQDEKSYINTGLNTQHINPQIDGWYNCGSNRKAWDYLVCNNLNQLARTAA
                     TSTRMMKSTFNEEISNNCIDFVKSSLVQSDVFTPYNLKSMKNNNIEHRLQVDIDSSLD
                     NPISKYIFKDVSDEAGEGVYAQDITSHLAVLQLSLQKTISNFENYKDITSKKIEELTK
                     RIEVLESR"
     gene            complement(6290..7045)
                     /locus_tag="PC472_001585"
     CDS             complement(6290..7045)
                     /locus_tag="PC472_001585"
                     /inference="COORDINATES: protein motif:HMM:NF017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDF2726912.1"
                     /translation="MALQYCVSENELVSFQYGEINSRDFGIVITDINELASPERRFER
                     IEIPGRNGSLILDEGCYSNFNLEIECYIDVDGKDINILASEIKMWLQTDFSYKNLIIS
                     TDPNYYREAVCINKLDLEEIIKDLGYFKITFECKPLKKELVNNLITINESNSKLINTG
                     MASYPLIKVFGNGDITLEINNEVIELTEVQGHIYIDCELMNAYKIDIHTNGVVNENSR
                     MFGDFPTFKHGENIITYSGDVEKIEIEPRWVVL"
     gene            complement(7206..7355)
                     /locus_tag="PC472_001586"
     CDS             complement(7206..7355)
                     /locus_tag="PC472_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726913.1"
                     /translation="MNNFLLNVIAGVIASLIFCLISKIFAKVKSHSVRKSGWEFDLKI
                     KFHKK"
     gene            complement(7483..13032)
                     /locus_tag="PC472_001587"
     CDS             complement(7483..13032)
                     /locus_tag="PC472_001587"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2726914.1"
                     /translation="MSLELGTAVGYLDLDGGKFFKALADAAKQLSQFNSSTKTTGSAM
                     TAMGKQISDTANSLTQIGRQSALAASELNKIASTSSGPFSSLKNSIKETENELRTARS
                     TIDTYGQGISRLTSEIDKSKQKYTEIGNKIERYEQQLERCNSMYGESSEQSQRYREAI
                     ERLKNSQTQLGNEIENGEDSLTDMRTAMNNAEAEANRLSDSLRSMPFDAIGGKMRDIG
                     QTLTSTVTTGLIAMGTAAVTAATNTEQAMSVVNSILQLNTEKVKGGKSEWDAYSSTLK
                     AGANEIGMAYDEYANAAYNAISASVKQADVTEFLAQADKLATAGLTDLAKATDVLTTV
                     QNAYGMSQKDMAHVSDVLIQTQNKGKITVDELASSMGKIIPTAKSLNVSVEQLGAGYA
                     ILTAKGIAGAEATTYMNAMFNELGKSGSKADKILKKLTDKGFADLQKEGKSTADVLLI
                     LNDYAKKNSLSLSDLFGSAEGAKAANVLLGDAVNETTGEIIEGTKSADSFNQMLGNMK
                     NSAGLCDKAFEQLDNTTKTKLEDALNSAKNMLSDLGEAMKPIISKVAEAITLFMNWAS
                     SMIQANPEIARIIVSIGAVVAAIGPLLVVLSALIINIPKIIALFGLAKGAIVSFGAAI
                     LASPAGIAVLVGVIAGLITMIGDNANALEFLQDKFGVFGTVLGTVCEFVAGITQMTFG
                     NLLILLKGVGEIIVALLSGKVWEIDDIWGKTWANIENNTAKAMSNIGAETSSALEKIK
                     NSTASQLSSVTSTFDLIMKEWPKLTRDNVGQVASTFAENLQGLDNQSIAILRGTSDSM
                     AVLFEGIYQGMDSTAATEKFASNLESMVISGKYSTETLQQDIDNAMRLINENVKDSSN
                     VLKQNATDVFNQFKTIGQAGFEGMAENVAGVMQGMDEQTISALTSMGGTWSDILKGVA
                     TDGSMSAEQMKNAIISNFNSMGIDGATLIDNLRKESSTYWKAMEEEANKAGSEVAKSF
                     DQVPKEVVTTLKNNGTESEEEILKLYNLYQQLPPEVQTYIKTNNMEALTGAQNVNDIL
                     AKIPPEIITNILSNVQGKEDVESTQMLLNALPKEERIKIETNVSGKENVTSVKQEIEA
                     LPKNANVNTNVNTGDSKEKLEEVSNKTKELNNVTGTLKVDANVSSAASKVSGLKTSVT
                     KDLNTTQANANKSTATMASNATNNFSKMASGAKSGTSKVATTTDADFKKANQSIQQSS
                     TAMYNGAKQSFTKLAQVAKQAGSDAYNGVNTSFSRLAQVVAISMSNAVTAANNSKSKF
                     KSAGSGAGQAWIDGFNSKKSAMRSAVSSVPKSVSFSFRSDEFIESNIKMKDSLAAGIN
                     KMSTFNNTSMRGSIFDEIMSTMNGTAKVFAGSVSQFKKAGENSAKAYLDGLSNIEEGL
                     TNTLRTVQAIIGNGNIKEAQEIKKFNKEVKKLQEEKAEEIAELDKEHKKKQKERNEKE
                     NEEIKEAEAKKYKNKKEKLAALKKIKDKYNKKSVKDQEEYREKLEKINKKYDKKEKED
                     TEKHQEELQKLREEKIKAERDFNKKYNDIKEDYAEKVANLDKKYIEDQKKLNEEYQKI
                     YDSRVKSLMDYTDLFSSVTFEEIDNDELMENLEEQVDVLRKWDTDMADLSTKVGKDLY
                     EELLAKGPQAHNEIEAINKMSKEQLEEYERLYQEKKKIAERRAKEDTEDEKYRIEKEI
                     EELKKTYDAEYRKLGQEMQRSIEEQIESFKDKFGVVPAMFMETGKDSMQGMIDGIKSM
                     QGALGKVLSDIVNSINKQLSNIGFKDVNIPTGGRKEAARFRNEIENLQSIAYSDTLAR
                     GIASNNLLKDATVNINNNSKVDSEKSSDKKVELTLHIEKFINNTKQDIEQLGSEIAFI
                     TNRKLKF"
     gene            complement(13118..13432)
                     /locus_tag="PC472_001588"
     CDS             complement(13118..13432)
                     /locus_tag="PC472_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726915.1"
                     /translation="MALFGDKVDKEDKKKLKEQEEIQKFMNKYQLQDLNEKDLTILRR
                     IADDLFATGLIKTGLALSFTKIEEQTKINYLSALVEQNWMIIRQLSQLNSNIEKLVED
                     KK"
     repeat_region   13609..13963
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13741..13768
                     /rpt_unit_seq="tttacataccacttagttaatataatac"
     gene            complement(14221..14763)
                     /locus_tag="PC472_001589"
     CDS             complement(14221..14763)
                     /locus_tag="PC472_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726916.1"
                     /translation="MKINKKILSLVLSLVIGIYAVGCSSPSSSDDSSKKDAKTEQKKD
                     EKKEYKFGDTIEMKDYDEGTYKLTFEGVRSTDERNEFAEIEAKKVVFVDFNYENVDCK
                     EAVFVTEGIEYQVADGDGNVLQTYPVDDSTRMQQETTAGTKSKGTTAFAITSDSNAVK
                     IIVKDKVNNKVMGEINATLE"
     gene            complement(14851..15048)
                     /locus_tag="PC472_001590"
     CDS             complement(14851..15048)
                     /locus_tag="PC472_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726917.1"
                     /translation="MPSEILRIKDEYTSFCFDEACMFLVDAIKNDKKLKFKENSNTSK
                     ENIKRTTFVDMALKKKEKDRK"
     gene            complement(15041..15517)
                     /locus_tag="PC472_001591"
     CDS             complement(15041..15517)
                     /locus_tag="PC472_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726918.1"
                     /translation="MNELKVTSLEELKKIKLTEVIEVGRFLDGTMLVAEVKQPDLMAL
                     AMAGKIPNSLMTASMSLVEEKESKDSTGEKVLKKMNDESNFSKEMFEMMDIVAKEVLV
                     NPTYAQIKKIGLELSIEQRLTLFNRIQGGTKSLENFHQESTDIEDSKSSDNVQQDA"
     gene            complement(15577..16383)
                     /locus_tag="PC472_001592"
     CDS             complement(15577..16383)
                     /locus_tag="PC472_001592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726919.1"
                     /translation="MAVQILNQYPLTDVVLVQIETVEQNPVTYTFDTSDEIGTEEIIS
                     EGEELTLKIKKKIIANRAAEDTSLGYDLTLKDNVFCPEILQIMQGGTIEKEEDGSFKR
                     YLAPEVGKTFSKKSFKTIIYSSVVGPGGDTGQFAKTTFPNCKGKSVPLNFKDGEYYSN
                     EYVINSRPNTGQSPYEVEIVEELPNGYEATKVFLDSASVSGATAGDKKITGLTAGKIY
                     KVTVNSNIKYTLADGTLTDTESDKAALTGTEIIGLTNGETYKVEEVSVSI"
     gene            complement(16389..16733)
                     /locus_tag="PC472_001593"
     CDS             complement(16389..16733)
                     /locus_tag="PC472_001593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726920.1"
                     /translation="MFKKIYKHLKNKGFNVYSIGQHQGLCIEPFLVIFEKGPLQTTEK
                     NIIKDLFEIYVFYPIGQYSKVSEYKISVESVMDEIVGIKQAYEALPILIDDEKQAYFT
                     RLSYYENKQIRR"
     gene            complement(16741..17175)
                     /locus_tag="PC472_001594"
     CDS             complement(16741..17175)
                     /locus_tag="PC472_001594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726921.1"
                     /translation="MSNGFKFDTSKLLNALVSREMKTKAALGAYADTSSQLLESTAKN
                     DRPWKDHTHDAKNRLHGSWEWQGDTIRIALSHGVDYGLYLEKGTGPHVIEARPGSYLF
                     WDGASHPVKKVNHPGSRPYPIIMPTIEKCAPSIIRGLDVILK"
     gene            complement(17177..17539)
                     /locus_tag="PC472_001595"
     CDS             complement(17177..17539)
                     /locus_tag="PC472_001595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726922.1"
                     /translation="MIAQKVKPKIIKAINKMPTEATVKRVGVNEFGEPSDEENIVCNM
                     IGLYHEGSSSISQITKDKGVVIKDKEQYLMVVCDEDTVKIKQGDFLYLDDNKFIIQDL
                     GNQNRMNIYFDLKLGKVR"
     gene            complement(17536..17790)
                     /locus_tag="PC472_001596"
     CDS             complement(17536..17790)
                     /locus_tag="PC472_001596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726923.1"
                     /translation="MDLEILKNIKTELREEQSPFFSDDEITYYYNKNNQDFNKTMYEL
                     CILKAENDSITLPGGLSMPENKLYWLTLAKKFKTNGSRCL"
     gene            complement(17816..18784)
                     /locus_tag="PC472_001597"
     CDS             complement(17816..18784)
                     /locus_tag="PC472_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDF2726924.1"
                     /translation="MAVTKLSDVIVPELFNPYVINKSMELSSLYQSGIITNDASLNAL
                     ASQAAPVVNMPFFEDLSGESEQIIEDADLTPNKITSSQDAAAILRRAKMWAATDLSAA
                     MAGKDPMAAIASLVGSFWARDMQKELIAVLNGVFLAANMTTNKLDISAGTGNAAKWSP
                     SAFIDAQQLLGDAQGQLKAIAMHSATKSALKKQNLIETIRPDVGPDFEVYQDKLVIVD
                     DGCPVSKEGVYTSYLFGSGAIALGNGNPVGFVATEIDRDKKKGSGVDYLINRKTYILH
                     PRGIKFTNASVAKTEGPSRLELAKGENWTRVYEPKQIRIVEFKHKL"
     gene            complement(18802..19437)
                     /locus_tag="PC472_001598"
     CDS             complement(18802..19437)
                     /locus_tag="PC472_001598"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2726925.1"
                     /translation="MEELLKKLGYQDTDIKNIIEGMKKEKIYTSKEENIDERYNKLKE
                     QKLALEEQIKGANDTISDLKKNSKNSEDIEAKVKEWENKYNELDSTSRAKIKNMTIDY
                     AINSKLSGVNEKYRKLLCKEFDTSKMEVKDTGEIIGLDEQFKDISETYKEWFESSTPS
                     NTGSPGNFPRKSNVVNNPFIKETFNLTEQGRLLKENPDKAKEFAAQAGINL"
     gene            complement(19573..19749)
                     /locus_tag="PC472_001599"
     CDS             complement(19573..19749)
                     /locus_tag="PC472_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726926.1"
                     /translation="MCNKFEDDKYLEPFSHPRWKDIPWGETIREVERTEEEQAQIRTK
                     FRKKLKEIGVIKED"
     gene            complement(19779..19994)
                     /locus_tag="PC472_001600"
     CDS             complement(19779..19994)
                     /locus_tag="PC472_001600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726927.1"
                     /translation="MLTIEEFIKLPDEEKGDAYKELSPHDMFLWRTQYSPIGFEVIGH
                     EEISEEDRIKNKKKFREHLKKIGVMEE"
     gene            complement(19995..20234)
                     /locus_tag="PC472_001601"
     CDS             complement(19995..20234)
                     /locus_tag="PC472_001601"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726928.1"
                     /translation="MYKLGEIVTNYDYTSTTKGSTYNSEGQVQMYILNSQKGKDVSKY
                     NPKEQEVLYKRGSMFKVIEIERIKGTIHILLEEVY"
     gene            complement(20266..20442)
                     /locus_tag="PC472_001602"
     CDS             complement(20266..20442)
                     /locus_tag="PC472_001602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726929.1"
                     /translation="MDNKFEDDKYSEPFSHPRWKDVPWGETIGYRDIPEEERKKYKKI
                     LREHLKDIGVLKED"
     gene            complement(20435..21853)
                     /locus_tag="PC472_001603"
     CDS             complement(20435..21853)
                     /locus_tag="PC472_001603"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726930.1"
                     /translation="MANKFNKAMKNAERARDISSNRTTKKIRKLYKDIANEYAKKLNR
                     VNSNTLTEQYLRESIIYLNKEYDRLGKKLKKDIESEISKVIKTTTDEQLSFFNNICDN
                     YSVNLKPQFTDMFSKVHEDVLRQVISGSMYKDKLKLSDRIWSNIDKTKKDLDYIVSRG
                     LAEKRGSYDIAKDLEKYVNPKVKKDYDWSKVYHKSNKKIDFNAYRLASTYITHAYQKT
                     AKESCKKNPFVKGIKWMSSHHPRMCKVCADRNGKTYIPEELPLEHPLGKCTFEYDIPM
                     SMEDIGKELRSWIDGEENSKLDEWFDEYGLDFAGIEGKANKNKEPTEDEMLALYKYMG
                     GDAYKINEKLRRNIKLTEEDEWFINNLDRVLDKMPNYEGDVTRSLYFYNKESLEAFLI
                     EHEIGKTIQYSEFISTTKGKTYNPEGQVEIYIFNSKNGKNISMYNEKEEEVLYRRNSK
                     FEIIELEEMNGKYYILMEERNG"
     gene            complement(21853..23337)
                     /locus_tag="PC472_001604"
     CDS             complement(21853..23337)
                     /locus_tag="PC472_001604"
                     /inference="COORDINATES: protein motif:HMM:NF016988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2726931.1"
                     /translation="MLDLIDIIQMELTGLYGQEVIREMGEIIRLYDKYEGTGQHWIEE
                     EQDYKQTRKKTNYIKKLIKEEARFLFGKTPIFTIKPENDLDKDKAEEINQCINKILKK
                     NLFSDKLIKAARDCFIGKRIAIKLHADKESKSLKIMFVPSLEFIYEPFDNQFDELKKI
                     VFFHQTNQEVEKDKQRIWKQKYEMVNDKCILSEGIYNGYGLCTEEIVSDTDLKLSGIP
                     CYVILNDGLLGDLKGESDIEEIFDNQMAYNKLASEDIDTLKKGMNRIIYGVDVEEESS
                     KHFKIKPGAYWDVETSQTADQKQAQINTIPTDFGYDTRIENSLNRIKSDMHEVLNIPL
                     INNQDLKGMMTSGKSMKALYWQLITRCEEKMKSWGPALEWLIQAMIEMIEVYNIVKIP
                     ILDKDSYEVIVENQYPLQEDEDSEKLLDIQQVNAQAMSRKTFIKKWNNSNDDIADEEL
                     QQISIERQILEESFNLEEETEAIDTEESDEVTNEKESKEVVDDE"
     gene            complement(23348..24613)
                     /locus_tag="PC472_001605"
     CDS             complement(23348..24613)
                     /locus_tag="PC472_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2726932.1"
                     /translation="MMDRKISEIINKNFYEFWKVSNSNKYLYHVLKGGRASAKSTHIA
                     FWLTMAMVKYPVNTVCFRKVGNTIMDSVYEQLKETIEIFGLTHLFQFKKSPMEIIFIP
                     RGNKFIFRGLDDPQKIKSIKSAKYPIAFAWFEEVAEIKTEDELSMVINSVLRGELPDK
                     LNYKIFLSYNPPKRKQSWVNKKFEIHVLPKNTYVHHSIYLDNPHISKAFIEEANEIKI
                     RNEFKYRWEYLGEPIGSGVVPFSNLEFKTITNEEIFQFDNIRQGNDFGYATDPMAFVR
                     LHYDKKKRVIYFIDEIFGVKMSIRELASKIKSKGYDDFSVICDSAEPRSIAELREYGI
                     KALKAKKGPGSIEFGENWLDDLQAIVIDPNRTPNIAREFENIDYQTDKDGNVRAKLED
                     KDNHSIDATRYALELDMKTHGRERKYNSR"
     gene            complement(24603..25043)
                     /locus_tag="PC472_001606"
     CDS             complement(24603..25043)
                     /locus_tag="PC472_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDF2726933.1"
                     /translation="MADLTEKQKRFCDYYIETGNATEAYKKAYKNNNQRTSESNGSRL
                     LSNDKVKNYIDERLKQIESKRIADAKEVMEYLTKILRNQEQEEVVIVSENGPEIIKKD
                     VSIKDRNKAAELLGKRYALWTEKVDLDGNVGVTIIDDVGNLNDG"
     gene            complement(25469..25645)
                     /locus_tag="PC472_001607"
     CDS             complement(25469..25645)
                     /locus_tag="PC472_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2726934.1"
                     /translation="MEKRILKVLLSKSGSGSLSPKISLPATWIKEMNITQEEREVEVY
                     FENNEIRIKKKDLD"
     gene            complement(26055..26555)
                     /locus_tag="PC472_001608"
     CDS             complement(26055..26555)
                     /locus_tag="PC472_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726935.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            complement(26574..26990)
                     /locus_tag="PC472_001609"
     CDS             complement(26574..26990)
                     /locus_tag="PC472_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDF2726936.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTVDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKIA"
     gene            complement(27017..27214)
                     /locus_tag="PC472_001610"
     CDS             complement(27017..27214)
                     /locus_tag="PC472_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726937.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            complement(27627..27989)
                     /locus_tag="PC472_001611"
     CDS             complement(27627..27989)
                     /locus_tag="PC472_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726938.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            complement(28019..28438)
                     /locus_tag="PC472_001612"
     CDS             complement(28019..28438)
                     /locus_tag="PC472_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2726939.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRTFTKVSTKSVQS
                     LESKSKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            complement(28458..29348)
                     /locus_tag="PC472_001613"
     CDS             complement(28458..29348)
                     /locus_tag="PC472_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2726940.1"
                     /translation="MDKVIIEKGNILSDGYGLMPRLVARDRWLTVGARMLYSYLSSFA
                     GINGTCFPTRDLICYELNISKDTFTKYKNELELSGYIKIHKNKSKQGKMQNNIYEIVF
                     DRTYIDECISRRGVKVNKKKKKPCPKNIDMEPCPTFPDTTQPDTENVDTNINSININS
                     FNSMYIDKPVDNYLKEFKKLYEENIGVVYPVTAEWLLEVSKEVDIRVFKRAIEICAER
                     MNMNLSYLKGILKKWKDANITTYEQLESYKLQHENKKSKKPNSVVSKNKFANFEQTFT
                     QYSNKELDEIIKKSQKAKFK"
     gene            complement(29367..30005)
                     /locus_tag="PC472_001614"
     CDS             complement(29367..30005)
                     /locus_tag="PC472_001614"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2726941.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            complement(29992..30273)
                     /locus_tag="PC472_001615"
     CDS             complement(29992..30273)
                     /locus_tag="PC472_001615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726942.1"
                     /translation="MKTKNEIIKDLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLQQFKENLEEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            complement(30352..30594)
                     /locus_tag="PC472_001616"
     CDS             complement(30352..30594)
                     /locus_tag="PC472_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726943.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            complement(30761..30904)
                     /locus_tag="PC472_001617"
     CDS             complement(30761..30904)
                     /locus_tag="PC472_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726944.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            complement(30891..31283)
                     /locus_tag="PC472_001618"
     CDS             complement(30891..31283)
                     /locus_tag="PC472_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2726945.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIVKP"
     gene            31453..31689
                     /locus_tag="PC472_001619"
     CDS             31453..31689
                     /locus_tag="PC472_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726946.1"
                     /translation="MNKDELNKICDEVYNRILKSEYSDETLLRNKISEFISLKEKSAS
                     EISNTELCMFFYFENIAFTKNYVKEVLSEILLDK"
     gene            complement(31647..31829)
                     /locus_tag="PC472_001620"
     CDS             complement(31647..31829)
                     /locus_tag="PC472_001620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726947.1"
                     /translation="MNEKDTKITEKDLHYITDYLLKESDIGKRLALLEEKTKPIKIDE
                     ISDYLSKRISDKTSLT"
     gene            complement(31877..32692)
                     /locus_tag="PC472_001621"
     CDS             complement(31877..32692)
                     /locus_tag="PC472_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2726948.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKRKKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFDEMEKALKNEQTKLPTTYKEALQHLIEQVEANEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQYQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLG"
     gene            complement(32723..32938)
                     /locus_tag="PC472_001622"
     CDS             complement(32723..32938)
                     /locus_tag="PC472_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2726949.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            33119..33664
                     /locus_tag="PC472_001623"
     CDS             33119..33664
                     /locus_tag="PC472_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2726950.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            33673..34089
                     /locus_tag="PC472_001624"
     CDS             33673..34089
                     /locus_tag="PC472_001624"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDF2726951.1"
                     /translation="MSEEQYLENRVNKQINWYSSKSSNIKKKFFTIQVIEIIIAGIIT
                     ALSKFSFENEHIADIITILSIVVIIFSSLRNLFKWQDLWIKYRYTAELLKREKFLFLT
                     RTNIYINENAFNLFTERIEDILNRENENWTELNLNS"
     gene            complement(34102..34323)
                     /locus_tag="PC472_001625"
     CDS             complement(34102..34323)
                     /locus_tag="PC472_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726952.1"
                     /translation="MQVKKFRKKPVVIEAYQTDIEFIIQTLEGPLKASIGDWIITGVR
                     GEQYPCKPDIFEKTYEAVDDEVPLTSFIV"
     gene            34387..34809
                     /locus_tag="PC472_001626"
     CDS             34387..34809
                     /locus_tag="PC472_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDF2726953.1"
                     /translation="MPKLYDYRVFISHAWKYGNDYDRLINLLNNANNFSYYNYSAPKE
                     KPLFPDGTPFSNSDIAKKITDKISPSQITIVISGMYVQYKDWIKYEIDESIRIGKPIL
                     GIRPYGNSNIPTYVSDNANKIVNWNTDSIVSAIRELVK"
     gene            34913..35419
                     /locus_tag="PC472_001627"
     CDS             34913..35419
                     /locus_tag="PC472_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2726954.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            35659..36789
                     /locus_tag="PC472_001628"
     CDS             35659..36789
                     /locus_tag="PC472_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2726955.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(37150..38838)
                     /gene="ilvB"
                     /locus_tag="PC472_001629"
     CDS             complement(37150..38838)
                     /gene="ilvB"
                     /locus_tag="PC472_001629"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2726956.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(38891..39886)
                     /gene="ilvC"
                     /locus_tag="PC472_001630"
     CDS             complement(38891..39886)
                     /gene="ilvC"
                     /locus_tag="PC472_001630"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2726957.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDHT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
BASE COUNT        11965 a         7299 c         4256 g        16548 t
ORIGIN      
        1 agattactat atttaagaat cctgaattat ttattattgt gtttactgct atttgtgagc
       61 ttgccacagc attcatgtat ttatcacttc tagctaacgc atccgaagtg ttgatattta
      121 tattgtctat ccccgctagc ttcgctgcgg tttttgctat agctaaatca atttccttga
      181 tagaattgaa agctacttcg tcacttgata aagctgttat aacacttaaa ttattttcta
      241 tgcattgaaa tatagcaatt atattatttt tgtctaagtt attatttgat aataaatctt
      301 ttatacttat aaaattagta ttcatatttt caactatagg aaaaatcttg tcttgatatt
      361 ctatgttagt tgaattaacc atattagtta taattgttat taaaggattc atatctaact
      421 taaatatact attatgagtt tttaaaatct ttgtaaatac aagtctaata acatttgtat
      481 ttgctaaaac taaatctaat gtatcttgat tagctaatag tagctgtgta gcttctaaat
      541 tctcaaatat cattctaact gattcatgat ttgttgatat atgagtcatt gcttcagtac
      601 tatttattat agcttctcta attacattat tttctaaaat cgcttttata acagctggtt
      661 ctgctgtaac ttttaaaatg tattgtccta gcaaatccat tgcgtttgaa cttgctacaa
      721 ctgcgtccat acttgtttta cacatagtta aagctactat tgcattacta ttattaagta
      781 ttgcattagt agcttccaca tttgctaata tttcttctat agttgtaaga ttttctaaat
      841 cagtattatc taagctaaaa gtagaattaa accatgctcc tatagttgac ttttcttgtt
      901 cccttgtaac atatctatca taccctgctt taaatttttc ctctaatgaa ttataatgct
      961 ctacagtcat tccttttact acattatcag catttcccaa actaaatata tctgttctgt
     1021 aaaaggcatt taattgttct cccagcttag tacctttatc aaacagatta tcaatgtctg
     1081 tttctgaaaa agctgtatac acaacttctc taattcctct atcagaaaat aaatataaac
     1141 tatttaagta catctcttcc caaatcctat gattaccttc atataaaaga taactttcag
     1201 aaccatttaa cttcaaatct tgtaaataat ttaaaaaatt taaaggttct ccttgaatat
     1261 cactatttat tttataaacc tctgcccaac tcactaaatc accccttcat ttgtaacagt
     1321 ttcttctatc attccatttt taaagttagt tattgttgta tttattaata tatcatttat
     1381 atacttttct tttgatatag aaccatttgt attaaacttt gtaatatact tgatattatt
     1441 atctaatatt tctattatag aaccatttgt attaaatttt gtttctttat ttttataagt
     1501 atctaattga gaaagttttt ctttttcttc agttgtataa tcttctgtag agagttcttt
     1561 gccttcaact ttctcaacat atatatcatg agtatgttct aaattggctt ttttatctaa
     1621 tccatctttt aataatcttt ttgtctctat actagaataa gcactttcaa gagactctat
     1681 atcatcatta atagtaaatt ttataagttc aatagcagaa ccaccattaa ctactgtaac
     1741 ttcaatgtct atagcaactt cattaaatag tactatatta ttatcatcta taattttgta
     1801 agatgtaatc aaacttttgt tattaagttt atctattaaa gagactaata ttttttcagt
     1861 aacaaggtta tgttctactg aaagttcaaa tcctccatca acttcaatcc attcagatgt
     1921 atcatatgat ttattaaatg ctacatttat tccaccagca ataagttgct ctatttttaa
     1981 atctagttta ttaaaatcat aagttaatct ctcttttaaa gtgtctttta tctgaccatc
     2041 agttgtttct tttgattgta atatttctcc tgctgtatct aaattttcta attcattgaa
     2101 ccttttttca aactcagtta ttttattgct aacagttgtt gtcatatctg tcttagcagt
     2161 atttacttca actattttgt tatcaacctc atctacttta tcatctactt ttttctgcat
     2221 attaattata gatgtctgcc tagttgattc atttagttct ctagttccct cattactttc
     2281 tctaagctct tcattctctt ctcgtttagt ctctgaattt tgtcttatta tttcatttgt
     2341 ttttcttgtt tcttcatttg tttcccttgt tgtttcacta acttttctag cttcttcatt
     2401 ggtatttctg gttgtttcac tagtttttct tatttcttca cttgcttttc tagcttcttc
     2461 attagtattt cttgtttctt caatagtgtt tcttacttct tcacttgctt ttctagtatt
     2521 ttcattactt tttcttattt cttcattttc ttctcttgtt tcttcattac tttttctaag
     2581 gtcttcgttt tcttctcttg tagtttctga ttcttgtcta gctacttcat taacaactct
     2641 tgtttcttcg ctaccatctc ttatctcttc acttgctttt ctagtatttt catttgattc
     2701 tcttgtttct tcactagttt ttcttatttc ctcatttgat tctctaacag tctcattttc
     2761 ttctcttgtt tcttcattag tatttctagt ttcttctctt gactctctgt ctacctcatt
     2821 tgtttttctt atttcttcat tagcttctct tgttatttca cttgtctttc ttatttcctc
     2881 attagcttct cttgttgttt cacttgtttt tcttgtttct tcattagctt ctctagtaac
     2941 ttcctgtgtt tttcttactt cttcactctc acatctttca acttcactat cttttctctg
     3001 attttcgctt tcaattctta cattctcatt gccaattcta acattttcag aacttattct
     3061 aatttcttca ttagattttc ttacattttc attttcttgt ctttcttctt cataacttaa
     3121 tacatttaat atgtccttta ttatatccca actaggctca tcctctattg catttctatt
     3181 tatacttttt tcaactttta ttattgtaga aaacgtagta actaccttat cgtttttata
     3241 taaagttatc tcagaaataa catctccttc ttctgaaagc tctatatgac ttaattcagt
     3301 ataaacagtt gaatcttcta tagtacaatt tttctgagta actcttccac ttggtaaatt
     3361 aaaaaatgct aatgctgtat atccagttaa atcaacatct atagagtctt gtactattct
     3421 catgttataa gatacattat tatcattttg cttatatctt gctatttgat acatctttgt
     3481 gttgatatct acaatataaa ttctatctct caagttatat cacctgcttt ctaatacttc
     3541 tattcgtttt gtaagttctt ctattttttt acttgttata tctttataat tttcaaaatt
     3601 tgatatagtt ttttgtaaag ataattgtaa tactgctaaa tgagatgtta tatcttgtgc
     3661 atagacacct tcaccagctt catctgatac atctttgaaa atgtacttag aaataggatt
     3721 atctaaagag gagtctatat caacctgtag tctatgttca atattgttgt ttttcataga
     3781 ttttaaatta tatggtgtga atacatcaga ttgtactaag ctacttttaa caaaatctat
     3841 acagttattt gaaatttctt cattaaatgt actcttcatc attcgagtag atgtagctgc
     3901 tgttcttgct agttgattta aattattaca tactaaataa tcccaagctt tcctatttga
     3961 accacaatta taccaaccat ctatttgagg atttatatgt tgtgtattca aacctgtatt
     4021 aatgtaactt ttttcatctt gataaccaaa ttcaaatact tcctctccat caagataacc
     4081 actaattaca tcatcagaaa catatatata tgaataatca ttatatttca tacgcattgc
     4141 ttttcctata cctactccaa actgagcagt agcatctaaa gcacattttg caccattccc
     4201 ctcaaatagt aatatgaatg ggtttgatga actagcacct attctgtcac ataaaatagt
     4261 acctgtgtct atataatcag catttatata taatctactt ccacttaaat atattccaca
     4321 atttcgattc cctgttaatg catcaaatac tgctttttgg tcactagtta aaacactttc
     4381 atagttattt ttatcaccta tagataactc attagctctt atattcactt ttccattact
     4441 atctacttgt aacgttgtat atccatcatt atctctgaca gttaaatttc taccatttat
     4501 aaattgacct tctaaagtac cagtttttat ataactagca tttagatata attcattccc
     4561 aaccatatag agtccctttg caagaccatt gttagttagt ttattaaata tctcaagttg
     4621 agttatatcg tctatttctc ccccaataat agattccaac gactttcctt ttaatgtaaa
     4681 ggtagttgct cctatatgaa catttccaaa actatctata tttaaagttg tttggctatt
     4741 tccatcaact acaactaaat tctttgcctc aatatactgc ccttttactt ttcctacatt
     4801 tatcttatct gcatctaaat ctcttatcac tgcatgacct atagctgctt cttcaaaata
     4861 ttcagctgca tcacttaact ttctagttgt tgcactaact tcatttgaaa attcattata
     4921 gttatcatgt gtatttcctg cccgtactct ataataccaa gtttgcatag gcttaacttc
     4981 atggagtaat gaacttgctt gacctacata tatcctattt gtatatccta tagtatctgg
     5041 ttcaaatcct tttatctgag atgcatatac ttcatagtta taatataact tattatcaaa
     5101 tgtccaatct aactgaatca tactccataa ccctaatgct gtgacatttg aaggctctgg
     5161 aagtgtatcc ggaaaattat cactttcaat tacaatatta tctacattat tttgaacctt
     5221 atctattttt tcttctaaat catctattct ttcgtcttta aaatgattta ctaagtcacc
     5281 aatttcaacc gaattgtatt tctttaatac tggattatat tcagttttta ctacccttgc
     5341 agtagcattt atacctaagt tatagtctct aactattatc tcatcatcca tatttacagt
     5401 ttcaagcatc ttatagcttt tataatcttc tgtagttgat aaatctacaa attctacttt
     5461 gtaagtaact ttaggtaaat caacattatt ttcaataaaa tagtctttac atttattcct
     5521 taagctttct tcattcttaa catcatcatc tgaaaaatca actgcaacaa ttctttgagt
     5581 tggataattg tttatatact tgctttctat atatttttct ggtagtgtaa tttttttatt
     5641 tttaccactt tttttagttg catatggata tatcttagtt ataacttctt gtgtatcata
     5701 agtagctgtt agacctatta tattcttcct atatgctagt aatacattgt tactttctcc
     5761 aatattattt aataaactta tattgaaatt atctcttttt agtttagact tattattaaa
     5821 taattcttgt atgtcagata tagcattatg tgggcttaca caagagatgg aaaagtcttt
     5881 atttcctgtt atatctgaat atcctacaaa tctattttct tcagtacatg acctaaatat
     5941 ttgattaaga gcttcttcac aggtaacatt tttaagttct aaattttcta taaaattatt
     6001 taataaatca taactaatgt gttctgcttt aactaatatt tttccatcaa gttccttaga
     6061 aatatagtaa atcctaaata attgattttt aagccttgga gaagcatcag ccataactat
     6121 tttattataa tcaatttcat caaataaaaa agagcccaca taatatgtaa actctaactc
     6181 aaatagtcca tttaattctt ctgtaacttt tgtttcaaat gcatctttta gaatccccaa
     6241 accattattt ttaaaatttg tttcatttgc tttatataat ctaagcaatc tataacacca
     6301 cccatctagg ttctatctct attttttcta catctccaga gtatgttatt atattttcac
     6361 catgtttaaa tgttggaaaa tctccaaaca tcctactatt ttcattaact acaccatttg
     6421 tatgaatatc tattttatat gcattcataa gctcacaatc aatatatata tgcccttgaa
     6481 cttctgtaag ttctatcacc tcattattta tttctaatgt tatatctcca tttccaaata
     6541 cttttataag aggatacgaa gccattccag tgtttattag cttagaattt gattcattta
     6601 ttgtaattaa attattaact aattctttct taagtggttt acattcaaat gtaatcttaa
     6661 aatatcctaa atcttttata atttcttcta aatctagttt gttaatacaa actgcttctc
     6721 tataatagtt tgggtcagta cttataataa gatttttata tgaaaaatca gtttgtaacc
     6781 acatctttat ttcagatgct agtatattaa tatctttacc atctacatct atatagcatt
     6841 ctatttcaag attaaaatta ctataacaac cttcatctaa aataaggctc ccattccttc
     6901 ctggtatctc tattctttca aatctacgtt ctggcgatgc aagttcatta atatctgtaa
     6961 ttacaatacc aaaatctcta ctatttattt cgccatattg aaaagaaact aattcatttt
     7021 cactgacaca gtattgtaaa gccaaattat cacctcctac acataaaaaa agagaggact
     7081 caaatttttc ttttgataca tctccttcta tttactttgc atatttttgc taagatgtga
     7141 tataataaaa gcaagaagaa ctacaatcta ttgtcagtag agcggagttc ataattaaaa
     7201 gctaattatt ttttatggaa tttgattttt aaatcaaact cccagccact ctttcgcaca
     7261 gagtggcttt ttacttttgc aaatatctta cttattaagc aaaatattaa gctagcaata
     7321 acaccagcta ttacattaag taaaaagttg ttcatactta ccacctcctt tcattagaaa
     7381 gtaggtttta tctcagtatg aactccactc ttagattata ggttacatct tcttgctaaa
     7441 agtattatac cacaatttgg atattaaaaa gatattatca cattaaaact ttagttttct
     7501 atttgtaata aatgctattt cagaacctaa ttgctctata tcttgtttag tattatttat
     7561 aaatttttct atatgtaaag ttaactcaac tttcttatca ctactttttt cactatcaac
     7621 tttactatta ttatttatat taacagttgc atccttcagt aaattatttg atgctattcc
     7681 tcttgccaga gtatctgaat atgctatact ttgtaaattt tctatttcat ttctaaacct
     7741 tgctgcttct tttcttcctc ctgtaggtat atttacatct ttaaatccta tattagaaag
     7801 ttgtttattt atagaattaa ctatgtcact taaaacctta cccaatgctc cttgcataga
     7861 tttaatacca tctatcatac cttgcataga atcttttcct gtttccataa acatagctgg
     7921 tactactcca aatttatcct taaaactttc aatttgttcc tctatacttc tttgcatttc
     7981 ttgacctagt tttctatact ctgcatcata agtttttttc aattcttcaa tttctttttc
     8041 tattctatat ttttcatctt ctgtatcttc ttttgctctt ctttcagcta tttttttctt
     8101 ttcttgatat aatctttcgt attcttctag ttgctctttt gacatcttgt taatagcttc
     8161 tatttcatta tgggcttgtg gtcctttagc taataattcc tcatataaat ctttacccac
     8221 ttttgtagat aaatctgcca tatcagtgtc ccattttcta agtacatcaa cttgctcttc
     8281 taagttttcc attaactcat cattgtcaat ttcctcaaat gttacagaag aaaataaatc
     8341 tgtataatcc attaaagatt ttactcttga gtcatatatc ttttgatatt cttcattgag
     8401 tttcttttgg tcctcaatat atttcttatc taagtttgct actttttcag cataatcttc
     8461 tttaatatca ttatattttt tattgaaatc tctttcagct tttattttct cttctctaag
     8521 tttttgaagt tcttcttgat gcttttcagt atcttctttt tcttttttat catacttttt
     8581 attaattttt tccaactttt ctctgtattc ttcttggtct tttacagatt tcttattata
     8641 tttatcttta atcttcttta gtgctgctag tttttctttt ttatttttat atttctttgc
     8701 ttctgcttcc tttatttctt cattttcttt ttcatttctt tctttttgtt tcttcttatg
     8761 ttctttatct aattctgcta tttcttcagc tttttcttcc tgtagctttt taacttcctt
     8821 attaaacttt ttaatttctt gtgcttcttt tatatttcca ttacctatta ttgcttgaac
     8881 tgttctaagt gtatttgtta atccttcttc tatgtttgac aatccatcta aatatgcttt
     8941 tgctgagttt tctcctgctt ttttaaattg tgagacagag ccagcaaata ctttagctgt
     9001 gccattcata gtactcataa tttcatcaaa aatactacct ctcatgcttg tattattaaa
     9061 tgttgacatt ttatttatac cagctgcaag agaatctttc attttaatat tagattcaat
     9121 aaactcatca cttctaaatg aaaaagatac tgactttggc actgaagaaa ctgcacttct
     9181 cattgcactt tttttactat taaatccatc tatccatgct tgaccagccc cagaaccagc
     9241 acttttaaat ttacttttag aattatttgc agctgttaca gcattactca tagaaatagc
     9301 caccacttgt gctaatctag agaatgatgt atttacacca ttgtatgcat cagacccagc
     9361 ctgtttagct acttgtgcaa gttttgtaaa tgattgttta gcaccattgt acatcgctgt
     9421 gcttgattgt tgtatagatt gattagcttt tttaaaatct gcatctgtag ttgttgcaac
     9481 cttacttgta ccacttttag ctccacttgc cattttgcta aagttattag ttgcattaga
     9541 agccattgta gcagttgatt tatttgcatt agcttgtgtt gtatttaaat ctttagtaac
     9601 agaagtcttt aatccactca cctttgaagc ggcagaactt acattagcat ctactttcaa
     9661 agttccagtc acattattta actcttttgt tttattagac acttcctcta gtttttcttt
     9721 actatctcct gtatttacat ttgtgttcac gttagcattt ttaggcaatg cttctatttc
     9781 ttgtttaaca cttgttacat tttccttacc acttacatta gtttcaattt ttattcgttc
     9841 ttcttttgga agtgcattaa gcaacatttg agttgattca acatcttctt tcccctgtac
     9901 attacttaaa atattagtta taatttctgg tggtatttta gctaaaatat catttacatt
     9961 ttgagcacca gttagagctt ccatattatt agtttttata taagtttgta cctctggtgg
    10021 caactgttga tatagattat ataattttaa tatttcttct tcgctttcag taccattatt
    10081 tttcagcgtt gtaacaactt ctttaggtac ttggtcaaaa gatttagcaa cttctgaacc
    10141 agctttattt gcttcttctt ccattgcctt ccaatacgta ctagattctt ttctcagatt
    10201 atctattaaa gtagctccat caatacccat tgaattgaag ttggatataa ttgcattttt
    10261 catttgttct gcactcatag agccatctgt cgcaactccc tttaatatat cactccaagt
    10321 accacccata gatgtaagag ctgatatagt ctgttcatcc ataccttgca tgactccagc
    10381 aacattttct gccattcctt caaatccagc ttgacctatt gttttaaatt gattaaatac
    10441 atctgttgca ttttgtttta aaacattact actatctttt acattttcat ttatcaatct
    10501 catagcatta tctatatctt gttgtaatgt ttctgtactg tatttaccac ttataaccat
    10561 gctttctaaa tttgacgcaa atttttctgt agctgctgta gaatccatgc cttgatatat
    10621 cccttcaaat aacactgcca tactatctga tgtgcctctg agtatagcaa ttgattgatt
    10681 atcaagccct tgcaaatttt cagcaaatgt acttgcaact tgacctacat tgtcccgtgt
    10741 aagtttaggc cattccttca ttattaagtc aaaggttgat gtaactgaac ttaattgact
    10801 tgcagttgaa ttttttattt tttctaaagc acttgaagtt tcagctccta tattcgacat
    10861 agcttttgct gtattatttt ctatattagc ccatgttttt ccccaaatat catcaatttc
    10921 ccagaccttc ccacttaaga gagctactat aatttctcct actcctttta gtagaattaa
    10981 taaattacca aaggtcattt gagttattcc agctacaaac tcacatactg ttcctagcac
    11041 agttccaaac actccaaatt tatcttgtaa aaattctaat gcatttgcat tatctcctat
    11101 catagttata agacctgcta ttactcctac aagaactgct atacctgcag gacttgctaa
    11161 aattgcggct ccaaaactta caatcgcacc ctttgctaat ccaaacaaag caattatttt
    11221 tggtatattt atgattaaag cacttaaaac aaccaataag ggacctattg ctgcgactac
    11281 agctcctata gacacaataa ttctcgctat ttctggattg gcttgtatca tactacttgc
    11341 ccagttcata aataaagtta ttgcttctgc cacttttgat ataattggtt tcatagcctc
    11401 tcctaaatca gatagcatat tctttgctga attcagtgca tcttcaagtt ttgtttttgt
    11461 agtattatcc agttgttcaa aagccttatc acaaagacca gcagaatttt tcatatttcc
    11521 cagcatttga ttaaatgaat cagcactctt tgtaccttcg attatttctc ctgtagtttc
    11581 attaactgca tcacctaaca gaacattagc cgcctttgca ccttctgctg aaccaaatag
    11641 gtctgataga gataaactat ttttctttgc ataatcattt aatattaata atacatctgc
    11701 tgttgatttt ccttcttttt gcaaatcagc aaatccttta tctgttaatt ttttaagtat
    11761 tttatctgct ttactcccgc ttttcccaag ctcattgaac attgcattca tgtaagttgt
    11821 tgcttctgcg ccagcaattc cttttgcagt taatatggca tatccagctc ctagttgctc
    11881 aactgataca ttcaaacttt ttgctgttgg tattatttta cccatagaac ttgctaactc
    11941 atccaccgta attttgcctt tattttgggt ctgtataaga acatcactta catgagccat
    12001 atccttctga gacattccat aggcattttg aacagttgtc aacacatctg tagcttttgc
    12061 taaatcagtt aatcctgctg ttgctagttt atctgcttgt gctaagaatt cggttacatc
    12121 tgcttgtttt acacttgcag atatagcatt atatgcagca ttagcatatt catcataagc
    12181 catcccgatt tcattagcac cagctttcag agtacttgaa taggcatccc actcactttt
    12241 tccaccctta actttttctg tatttagttg tagtatagag ttaacaactg acattgcttg
    12301 ttctgtgttt gtagctgctg ttactgctgc tgttcccatt gcaattagtc cagttgtaac
    12361 tgtagaagtt agtgtttgac caatatctct cattttgcca ccaatagcat caaatggcat
    12421 acttcttaat gaatcactaa gtctattagc ttctgcctct gcattattca tagctgttct
    12481 catatcagtt aaagaatctt ctccattttc gatttcatta cctaattgag tttgagaatt
    12541 tttaagtctt tcaattgctt ctctatatct ttgtgactgc tctgaacttt ctccatacat
    12601 actgttacaa cgttctaatt gttgttcata tctttctatt ttgtttccta tttctgtata
    12661 cttttgcttc gacttatcta tttcggatgt aagccttgat attccttgtc cataagtgtc
    12721 tattgtactt cttgctgttc ttagttcatt ttcagtttcc tttatagaat ttttcaaaga
    12781 tgaaaatggc ccacttgaag ttgatgctat tttatttaat tctgatgctg ctaacgctga
    12841 ttgcctacct atttgtgtca atgagtttgc tgtatcagaa atttgtttac ccattgccgt
    12901 cattgcacta cctgtggttt ttgtacttga attaaattga ctaagttgtt tagctgcatc
    12961 tgcaagagct ttaaaaaact tccctccatc taaatctaga taacctactg ctgttcctaa
    13021 ttctaaggac atatttttcc ctccctccct caaaatttca tataaaaaaa cacctactat
    13081 ttaagtaagt gttttctgta ttattatttt cagtccatta ttttttatct tctacaagtt
    13141 tttctatatt gctattcaat tgagataatt gtctaattat catccagttt tgctcaacta
    13201 aagcactaag ataatttatt ttagtttgtt cttctatttt tgtaaaactc aatgctaatc
    13261 ctgtttttat aagtccagtt gcaaataaat catccgctat tcttcttaaa atagttaaat
    13321 ctttttcatt taaatcttgt aattgatact tattcatgaa tttttgtatt tcttcttgct
    13381 cttttaattt ctttttatct tctttatcaa ctttatcccc aaataatgcc ataacattct
    13441 ccttcactat aaattaaatg tattttctat ttcaattata taatatatta ataatttttt
    13501 cattaattat ttgtaatatt attccataat aatttcacat aactgattaa atttaagcac
    13561 aacaaaaaca cttactattt aaataagtgt tttctgtatt tgttcaaaat ttaagtccac
    13621 atagttaata taatacctgc taatgctcca aatgctaaag ttaatgctcc catctttaca
    13681 tactgcttag ttaatataat acatggtgta aagacataga ttctaaattt tcaacttggc
    13741 tttacatacc acttagttaa tataatactt tttttaggca agttatggga aagtattaaa
    13801 gaaactttac ataccactta gttaatataa tacctgttgt ttcttgttgc attctagaat
    13861 agtcactaac atttacatac cacttagtta atataataca ttaaatttag aacaataaaa
    13921 ttatatttct gcaaatttac ataccacata gttaatataa taccccaaaa taaattgagc
    13981 acttccagtt cttgcacata taaaaccata ttaaaattgc agtgagtaac cagtagtgtt
    14041 tttcacacac cacaaaacat gcttatattg tagtaatttc aatgattaca tccaatttat
    14101 ataaaaaatc gtacactgca aaatatctat attatcatta tatcataaat taacattaat
    14161 ttattctatt catttagaat attttacctg ttaacttttt tcttttctct aatactattt
    14221 ttattctaaa gtagcattta tttctcccat gaccttatta tttactttat cttttacaat
    14281 tatttttaca gcatttgagt ctgatgttat agcaaacgct gttgttccct ttgatttagt
    14341 acctgctgtt gtttcttgtt gcattctagt tgaatcgtct actggataag tttgtagcac
    14401 atttccatca ccatctgcca cttgatattc aattccctca gttacaaata cagcttcttt
    14461 acaatctaca ttttcatagt taaaatcaac aaaaactact tttttagctt caatttcagc
    14521 gaactcatta cgttcatctg tgcttctcac accttcaaat gttagcttgt aagttccttc
    14581 atcatagtct ttcatttcta tagtatctcc aaatttatac tctttttttt catctttctt
    14641 ttgctctgtc tttgcatctt ttttagatga atcatctgac gaacttggag aactgcatcc
    14701 aactgcataa atcccaatta ctaacgatag aactaatgat aatatttttt tgtttatttt
    14761 cataatataa tcccccagtg aagttatttt ctcattataa attttacact aaatcacaga
    14821 gttagtctat tataatagaa taaattaata ctattttcta tctttttctt tcttttttaa
    14881 tgccatatca acaaaagtag ttctttttat attttcttta gaagtattac tattttcttt
    14941 aaatttaagc tttttatcat tttttatagc atctactaaa aacatacaag cttcatcgaa
    15001 acaaaaagaa gtatattcat ctttaattct taatatctca ctaggcatct tgttgtacat
    15061 tatcacttga tttagaatcc tcaatatctg tggactcttg atgaaagttt tctagtgact
    15121 ttgtacctcc ttgaatacga ttaaataatg ttaatctttg ttcaatagaa agttccaatc
    15181 ctattttttt aatttgagca tatgtagggt taactagcac ttctttagcc actatatcca
    15241 tcatttcaaa catttctttt gagaaattag actcatcatt catcttcttt agtacttttt
    15301 ctcctgtact atctttactt tccttctctt caactaaact cattgatgct gtcattaggc
    15361 tgtttggtat cttaccagcc attgcaagag ccattaagtc aggttgtttt acttctgcaa
    15421 ctaacatagt tccatctaaa aatcttccaa cctctattac ctcagtcaat tttatttttt
    15481 tcaattcttc taaacttgtt actttaagtt cattcatatt tatgtcccct ttctaaaaaa
    15541 agctctagct aattactaga gctttaattt atctatttat atacttacac taacttcttc
    15601 aaccttatat gtttctccat ttgttaatcc tattatctct gttcctgtta atgctgcttt
    15661 atcagactca gtatctgtta atgttccatc tgctaaagta tattttatat tgctattaac
    15721 tgtaacctta tatattttgc ctgctgttaa tcctgttatc tttttatctc ctgctgttgc
    15781 tcctgataca gatgcactgt ctaaaaatac ttttgtagct tcataaccat taggtaattc
    15841 ctctactatt tcaacctcat aaggagattg ccctgtgtta ggtctactat ttataacata
    15901 ttcatttgaa taatattctc catctttaaa gtttagtgga acagattttc ctttacaatt
    15961 tgggaaagtc gtcttagcaa attgcccagt atctcctcct ggtccaacaa ctgaactata
    16021 aattattgtt ttaaaagatt tcttagaaaa agtcttccct acttctggag ctaaatatct
    16081 cttaaagcta ccatcttcct ctttttctat tgttccacct tgcattattt gaagtatttc
    16141 aggacaaaat acattatctt ttaatgtcaa atcatatcct aaacttgtat cttctgctgc
    16201 tctatttgct attatttttt tctttatttt taaagttaat tcttcacctt cagaaatgat
    16261 ttcctctgtc cctatctcat cagatgtatc aaatgtatat gtaaccggat tttgttcaac
    16321 tgtttcaatt tgaactaata caacatctgt aagtgggtat tgatttaata tttgtactgc
    16381 catatttatt acctcctaat ttgtttgttt tcataataac ttaatctcgt gaaataagct
    16441 tgtttttcat catctataag aataggtaaa gcttcataag cttgttttat tcctactatt
    16501 tcatccatga cactttcaac acttatcttg tattcactta ctttagaata ctgtccaatt
    16561 ggataaaata catatatttc aaataaatct tttattatat ttttttctgt tgtttgtaga
    16621 ggaccttttt caaatataac taaaaaaggt tctatgcata atccttgatg ttgtccaata
    16681 gaatacacat taaaaccctt attttttaaa tgtttatata tttttttaaa catacttcac
    16741 ctactttaaa atcacatcaa gacctcttat aatacttggt gcacattttt ctatagttgg
    16801 cattataatt ggatatggtc tacttcctgg atgattaact tttttaacag gatgtgatgc
    16861 tccatcccaa aataaataac ttccaggtct tgcttctata acatgtggac ctgtaccttt
    16921 ttctaagtat aatccatagt caactccatg actaagtgct atccttatag tgtctccttg
    16981 ccattcccaa ctaccatgta gcctattttt agcatcatga gtatggtctt tccatggtct
    17041 atcgtttttg gcagtgcttt ctaatagttg agatgaagtg tctgcataag ctcctagtgc
    17101 agccttagtt ttcatttctc tacttactaa agcattaagc agcttgctag tatcaaattt
    17161 aaatccatta ctcatactat ctcacctttc ctaactttaa atcaaaataa atattcattc
    17221 tattttgatt tccaaggtct tgtataataa acttattatc atctaaatac aaaaaatcac
    17281 cttgctttat ctttactgta tcttcatcac aaacaaccat taaatactgc tctttatctt
    17341 ttataacaac acctttatcc tttgttattt gacttataga gctacttcct tcatgatata
    17401 aacctatcat attacaaact atattttctt catctgaagg ctctccaaac tcatttactc
    17461 caactctttt tactgtagct tctgttggca tcttattgat agctttgatt atctttggtt
    17521 ttaccttttg agctatcata gacatctact tccatttgtt ttaaactttt ttgccagagt
    17581 taaccaatat agtttatttt ctggcatgct taagcctcca ggtaaagtga tactatcatt
    17641 ttcagctttt aatatacata gttcatacat agttttatta aaatcttgat tgtttttatt
    17701 gtagtaataa gtaatttcat catcagaaaa aaaaggtgat tgttcttctc taagttctgt
    17761 ttttatattt tttaagattt ctaaatccat actatcacct actaaaaact atattttata
    17821 acttatgctt aaattcaact attcttattt gcttaggctc ataaactctt gtccagtttt
    17881 caccttttgc taactctaat cttgaaggac cttctgtttt agctacactt gcattagtaa
    17941 attttatacc tcttggatgt agtatatatg tttttctatt gattaaataa tctactccag
    18001 aacctttttt cttatctcta tctatttctg tagcaacaaa ccctactgga ttaccatttc
    18061 ctaaagctat tgccccacta ccaaatagat aagaagtata aactccttct ttagatacag
    18121 gacaaccatc atcaactata actaatttat cttgataaac ttcaaaatct ggacctacat
    18181 ctggtctaat agtttctatt aaattttgtt ttttaagtgc tgattttgta gctgaatgca
    18241 ttgctatagc ctttaactga ccttgtgcat ctcctaataa ctgttgtgca tctataaaag
    18301 ctgatggtga ccattttgct gcatttcctg ttcctgctga tatatctaac ttatttgttg
    18361 tcatatttgc agctaaaaat acaccattta aaactgctat aagttctttt tgcatatctc
    18421 tagcccagaa gcttccaact aaactagcta ttgctgccat tgggtctttt cctgccatag
    18481 ctgctgataa atcagtagct gcccacattt ttgctcttct taatatagct gctgcatctt
    18541 gacttgaagt aattttattt ggtgttaaat cagcatcttc tattatttgc tctgattctc
    18601 cacttaaatc ctcaaagaaa ggcatattta caactggtgc agcttgagaa gctaaagcat
    18661 ttaagcttgc atcatttgta attattccac tttgatatag agatgataac tccatgcttt
    18721 tgtttattac atatgggtta aacaattctg gtactattac atcacttaat tttgttactg
    18781 ccatttaaat cactctcttc cttataaatt tattcctgct tgagctgcaa attctttagc
    18841 tttatcaggg ttttctttta ataatcttcc ttgttctgtt aaattgaatg tttcttttat
    18901 aaaaggatta ttgactacat ttgattttct tgggaaattc cctggagaac ctgtattact
    18961 tggagtagaa ctttcaaacc attctttata tgtttctgat atgtctttaa attgttcatc
    19021 taatcctata atttctccag tatcttttac ttccatttta ctggtatcaa attctttaca
    19081 taggagcttc ctatattttt catttactcc agatagttta gaatttatag catagtctat
    19141 agtcatattc tttattttag ctctactcgt actatcaagt tcattatatt tattttccca
    19201 ttctttcact tttgcctcta tatcttcact gtttttacta tttttcttca aatctgatat
    19261 agtgtcattt gcacctttta tttgctcttc taaggctaat ttttgttctt tcaacttatt
    19321 gtatctttca tctatatttt cttccttaga agtgtagatt ttctcttttt tcataccctc
    19381 aattatattt tttatgtctg tgtcttggta ccccaatttt tttaataatt cttccataat
    19441 tccctcctac actacgcttt tatacgagtt ttgcctctct agtatagttt gctatttatt
    19501 tcttttacgc ctatatatag ctaaaaaagg caataaaaaa acacctacta atttttaatt
    19561 tcagtaagtg ccttaatctt cttttattac tccaatttct tttaatttct ttctaaattt
    19621 tgttcttatt tgtgcttgtt cctcttcagt tcgctctact tccctaatag tctcccccca
    19681 cggaatgtct ttccatcttg gatggctgaa aggttctaag tacttatcat cttcaaattt
    19741 attacacatt ttatagctcc tctgactatt ataaatattt actcttccat tacacctatt
    19801 ttcttgagat gttctctaaa cttctttttg ttcttaatcc tatcttcttc tgagatttct
    19861 tcatgaccta tcacttcaaa tccaattggt gaatattgtg ttctccataa aaacatatca
    19921 tgaggagata attctttata agcatctcct ttttcttcat caggtagctt tataaattct
    19981 tctattgtta gcatttaata cacttcctct aataaaatat gaattgttcc cttaattctt
    20041 tcaatttcaa taactttaaa cattgaacct cgtttatata atacttcttg ttctttaggg
    20101 ttatatttac ttacatcttt tcctttttgt gaatttaata tatacatctg cacttgaccc
    20161 tcagaattat aagtacttcc ttttgttgtt gaagtatagt cataattagt aactatctcc
    20221 cccaacttgt acatacttac gaatctttaa tttaagtaag tgtctttaat cttcttttaa
    20281 tactccaata tcttttagat gttctctaag tattttttta tatttttttc tttcttcttc
    20341 tggaatgtct ctatatccta tagtttcacc ccatggtaca tctttccatc ttggatgact
    20401 aaatggttct gaatacttat catcttcaaa tttattatcc attgcgttcc tccattaata
    20461 tataatattt gccattcatt tcttctaact caattatttc aaatttagaa tttcttctat
    20521 acaaaacttc ttcttctttt tcattgtaca tacttatatt tttaccattc ttagaattga
    20581 atatatatat ttctacttga ccctctggat tgtaggtttt tccttttgta gtcgagataa
    20641 attctgagta ttgtatagtt tttccaattt catgttcaat taaaaaagct tctaaacttt
    20701 ctttattata aaaatataat gaccttgtaa catccccttc ataatttggc attttatcta
    20761 aaactctatc taagttattt ataaaccatt catcttcttc tgtcaattta atatttcttc
    20821 ttaatttctc attaatttta taggcatctc cacccatata tttatataaa gctaacattt
    20881 catcttcagt tggttcttta ttcttattag cttttccttc tattcctgca aagtctaatc
    20941 catattcatc aaaccattca tcaagtttag agttttcttc tccatctatc cagcttctta
    21001 actctttacc tatgtcctcc atactcattg gaatatcata ttcaaaagta cactttccta
    21061 aaggatgctc taatggtaac tcttctggaa tatatgtttt tccatttcta tctgcacaaa
    21121 ccttacacat cctaggatga tgtgatgaca tccatttaat ccctttaaca aatggattct
    21181 tcttacaact ttctttagct gtcttttgat atgcatgagt tatatatgta gatgccagtc
    21241 tataggcatt aaaatctatt tttttgttac ttttatggta tacctttgac caatcataat
    21301 ctttcttaac ctttggatta acatactttt ctaaatcttt tgctatatca taactacctc
    21361 ttttttctgc taatcctcta cttacaatgt agtctaaatc cttcttagtt ttatctatat
    21421 tactccaaat tctatcactt aatttaagtt tgtctttgta catgctacca gatataactt
    21481 gccttagtac atcttcatga actttactaa acatatcagt gaattgtggc tttaaattaa
    21541 ctgaatagtt atcacatatg ttattgaaaa aacttaattg ttcatctgta gtagttttta
    21601 ttacttttga tatttcactt tcaatatctt tttttagctt ctttcctaac ctatcatact
    21661 ctttatttaa ataaattata ctttctctta aatattgttc agttaatgtg ttagagttaa
    21721 ctctatttaa tttttttgca tattcattag ctatatcttt atataacttt cttatctttt
    21781 tagtagttct atttgatgat atgtctctag ctctttcagc attcttcata gccttattaa
    21841 acttattagc cattattcat catcaactac ttcttttgat tctttttcat tagttacttc
    21901 gtctgattct tctgtatcta tagcttctgt ttcctcttcc aaattaaaac tttcttctaa
    21961 tatttgtctt tcaattgata tttgttgcag ttcttcatct gctatatcat cattactatt
    22021 attccacttc ttaatgaaag tcttcctaga cattgcttga gcatttactt gttgaatatc
    22081 aagtaatttt tctgaatcct catcttcttg taatggatat tgattttcaa caattacttc
    22141 ataagaatct ttgtctaata taggtatttt tactatatta tatacttcta tcatctctat
    22201 catggcttgt atcaaccatt caagagctgg accccatgat ttcatttttt cctcacatct
    22261 agttattaat tgccaataca aagctttcat tgatttacct gatgtcatca tacctttaag
    22321 gtcctgatta tttataagag gtatatttag tacttcatgc atatctgatt taattctatt
    22381 aagagagttt tctattctag tatcatatcc aaagtcagta ggaattgtat taatctgtgc
    22441 ttgtttttgg tcagccgttt gagaagtttc tacatcccaa taagctcctg gttttatttt
    22501 aaaatgctta cttgactctt cttcaacatc aacaccatat atgatacgat tcattccttt
    22561 tttaagagta tctatatcct cagaagctag cttattataa gccatctgat tatcaaatat
    22621 ctcttctatg tcactctcac cctttaaatc accaagcaaa ccatcattaa gaattacata
    22681 acaaggtatt ccactaagtt ttaaatctgt atcactcact atttcttctg tacataaccc
    22741 ataaccatta taaatacctt cacttaatat acatttatca ttaaccattt catatttttg
    22801 tttccaaatt ctctgtttat ctttttcaac ttcttgattt gtttgatgga aaaatacgat
    22861 tttcttgagc tcatcaaatt ggttatcaaa tggttcatat ataaactcta aacttggtac
    22921 aaacattatc tttaaactct tactttcttt atctgcatgt agctttatag ctattctttt
    22981 accaataaaa caatctctag ccgctttaat tagtttatct gaaaacaaat ttttctttag
    23041 aatcttatta atacactggt ttatttcttc tgccttatct ttatctaaat cattctctgg
    23101 ttttattgta aatattggtg tcttaccaaa taaaaatcta gcttcttctt taatcagctt
    23161 tttaatataa tttgttttct tccttgtttg tttataatct tgttcctctt ctatccaatg
    23221 ttgtcctgta ccttcatact tatcatatag ccttataatt tcacccattt ctcttattac
    23281 ttcttgacca tacaatcctg taagttccat ctgaattata tctatcaaat ctaacatgtt
    23341 acacccccta tctactgtta tattttcttt ctcttccatg tgttttcata tctaactcta
    23401 atgcatatct tgttgcatca attgaatgat tgtctttatc ttctaactta gctcttacat
    23461 ttccatcctt atctgtttga taatctatat tttcaaattc tctagctata tttggagttc
    23521 tgtttgggtc tattactatt gcttgtaaat catccagcca gttctctcca aattcgattg
    23581 aaccaggtcc ttttttagcc ttcaatgctt ttattccata ttctctaagc tctgcaatac
    23641 ttcttggttc tgcactgtca caaataacac taaagtcatc atatcctttt gatttaatct
    23701 tagaagctaa ttctcttata gacattttca ccccaaatat ttcatctata aaatatataa
    23761 ctcttttctt tttgtcataa tgcagtctta caaatgccat agggtctgtt gcatatccaa
    23821 agtcattacc ttgccttatg ttgtcaaact ggaatatttc ttcatttgtg attgttttaa
    23881 actctaaatt agaaaaaggg actactccag aaccaattgg ctctcctaag tactcccatc
    23941 tatatttaaa ctcatttctt attttaattt cattagcttc ttcaatgaaa gcctttgata
    24001 tatgaggatt atctaaataa atactatgat gaacatatgt atttttaggt aatacatgta
    24061 tttcaaattt cttattaacc catgattgtt ttctttttgg aggattgtat gataggaata
    24121 ttttataatt caatttgtct ggtaattctc cacgtaacac tgaattaatt accatagata
    24181 actcatcctc tgtttttatt tctgcaacct cttcaaacca tgcaaaagct attggatact
    24241 tagctgattt tatagatttt atcttttgtg ggtcatcaag ccctctaaat ataaacttat
    24301 ttcctcttgg aataaaaata atttccattg gggatttttt aaactgaaat aaatgtgtta
    24361 aaccaaatat ttctatagtt tctttcaatt gctcatatac actatccatt attgtattac
    24421 caactttcct aaaacaaaca gtatttactg gatatttaac catagccata gttaaccaaa
    24481 aggctatatg tgttgactta gctgaagctc ttccaccttt taatacatga tataggtatt
    24541 tgttactatt acttactttc caaaattcat aaaaattttt atttattatt tcagatattt
    24601 ttctatccat catttaaatt acctacatca tcaattatag ttacaccaac atttccatct
    24661 aaatcaactt tttcagtcca taaagcatac cttttaccca ataattcagc tgccttattt
    24721 ctatctttta tacttacatc tttttttatt atttcaggac cattttctga tactattaca
    24781 acttcttctt gttcttgatt tcttagtatc tttgttaaat actccataac ttcttttgca
    24841 tctgctatcc tctttgattc aatttgcttt aatctttcat caatgtaatt tttaacctta
    24901 tcattactca gcagtctact tccattactt tctgatgttc tttgattgtt atttttgtat
    24961 gctttcttgt atgcttctgt tgcattacca gtttcaatat aataatcaca gaaccttttt
    25021 tgtttttcag tcaaatctgc cataccacca cctctttatt tgctttataa ataaaaaaat
    25081 aagactttaa atttaatcta aagccttatt cttaggggat acatattata tttaagggag
    25141 caagttttag gaatcgaacc taagattaca caccagtcct tgcaaattga gtgaggttac
    25201 caagccccac tcttttagac atttgaatta aattccgttt taatccagca tatctacata
    25261 tagtgtatta ataagtttga acatagtaag aattgaactt acagcatcct catgccctgc
    25321 ctagtctgtt catataagct aggtgaatcc ctttacctag cccacatata tttagttttg
    25381 agagagaaat attcatttcc acaatactat tatctcacac tttaaattgt aaaatcggca
    25441 gaaaaacggc aataaaaaga cctagaattt aatctaggtc tttcttttta atcctaattt
    25501 cattattttc aaaataaact tcaacttctc tctcttcttg tgttatattc atttctttaa
    25561 tccatgttgc aggtagactt attttaggac ttagtgaacc agagccactt tttgataaga
    25621 gtactttcaa tattcttttt tccatttctt tctctccctc taggaaaata aatattttat
    25681 agcaaatatt aataatgcta ttgcaccaat taatttaact ataccaaaac ttaatttccc
    25741 taattctgta attaattcct tatattttcc cattgtattt tgagtggtca tgtattatta
    25801 tttaattaga agggaaggtg ctgactcccc ttctaaagtc ttataggcta tctaatacca
    25861 tttttataac ggctattagt gtacctattt caagagcaag ttcggtaagg gctttgacaa
    25921 ctttgccgaa ctcttttatt tttttgacca cttctttcac ctcctttcta tatttatatt
    25981 atatcatgtc gtaccgatat aatcaatacc tttttccctt tttttagtaa aaaaaataga
    26041 cagctattca ctgcctataa atctaacatc ttaaataatg gttcttgctc tattagtgct
    26101 ttctttccaa acaaggctat tgatattgaa ctaatggctt gattagctct ttctcttaat
    26161 tgtctttctt ctaagtatac tttatcaact attaaactcc attctaagcc ttcaatatac
    26221 ctatacctta taatttgttt atgtataggt tttaaattgc ttatggatac atctattgta
    26281 tattttagtg cttccatttc atataattct atctgctttt ctattatctt ttcttcaaga
    26341 ttaattaact cattttcaac ttgattactt attgaattag tcttacttat gggaatgctg
    26401 tcatagctta aaccttgcat aaaatcacct aaatggaact ctttaagatt ttttatttga
    26461 agttttagac tttcaatatt aatatgtagt tgtttgtagt tctcaaggtg ttttttagtt
    26521 gccataaaaa actccttttt aactttactt gccataacat cactcctatt tatttaagct
    26581 atctttttct tattttcttt tctctttttc ttaagttcac tatattctat ccaaccgtct
    26641 accccatatt ttttgctttt agcaatccat atcaattttt tgtcctgata tttatagtca
    26701 aaaagctttt ttctaagttc accctgctgt gtactatacc ctttcacatc tatataaacg
    26761 acttcaccat tccatttgta tatggcaaaa tcaactgtat atgtaatagc tctgtagctt
    26821 ttcccatctt ttttaaattt aggttgtagt tcaaactttt gttgaagtcc aaagtctttt
    26881 atttctccat tttccttttt ttcttttaaa tataaataat actctgactc atctttacta
    26941 tcaaatttaa ttccatctat tacaattttc ttattattgt atttactcaa tcaaatactt
    27001 cctttatcac ttaaagttac tcatacttat taatatcgtt tataaattca taaacctcat
    27061 gtacactata tccaaacttt tcaaaccctt ttacatatct tcttatggaa cttgatatac
    27121 ttctacctac tatacaattc tcatatcctt gcaacaagtt ttctttttcc tcttgcttca
    27181 ccatttgaga aactgctttt tgaaacttat tcattgctat tcctccatag ttttattgtt
    27241 taaaattaat tttgctgttc tctattgctt ttattagcct ttctctcaca tcttttacaa
    27301 cagtaaattt ttttagactg ttttgggatg taaaataatt taccacacca attgcaaatt
    27361 attcttttat tcataaaacc acttctttct catttttcag gatatttgtt attacaattt
    27421 tcacactctt ttagattcaa tctatactca taaactctac cagctataaa acttcctatt
    27481 actaatatca cactagctaa gatattcact tttaatcatc tcctcatatt cttctctagc
    27541 cttatttata gcaataaata tatcctctcc attgtcatat aactcttttg cccttttaat
    27601 tgtatattct gtccttgaaa cttccattat tcctccttaa tatattcaac tttccagcca
    27661 cttcttgttt tagttttctt tttaatagct tgataaactg cttgatattt taaccttaaa
    27721 aaacaggctg caccatctat agactcaaat tcttttactt cccctgtatt aacatttcta
    27781 attcttactg gtaactcttt ttttattttt ctgtccttct ttactccaaa ctcagctaac
    27841 attttttctg gcttaggata tatctttttt ccattttttg taattcccaa caaacaacaa
    27901 tataatgcta agtagtttct acatgtaagg tcatcttcaa taaggttgtc cactactgag
    27961 ccactaaaat acctttctac tttaaacatt tatttcacct catatttaat ttttcagatt
    28021 aaaatggtaa ctcatcatca tctataattt caaatccttg agggtctagt cctatggttc
    28081 catcttgtac actctcttta tatgaattac tcgatttact cttgctttct aatgattgta
    28141 ctgattttgt actgactttt gtaaatgttc ttttttcacc tgattgagtt tgataattgt
    28201 caactcttat attcccttct aatgctacta gcttcccttt tgttatgtaa tttgcacagt
    28261 attcagctga tttacctatt acttctattg gtataaaatc agtatccctt tttccttctt
    28321 tatttatata atttctgtct acagctattg taaatgatgc tactgctgta cctgttcctg
    28381 gtatgtattt gagttctggg tctctagtta atcttccaac taatacaact tgattcattt
    28441 taatacctcc atcaatatta tttaaattta gccttttggc ttttctttat aatttcatct
    28501 aattctttgt tactgtattg agtaaatgtt tgttcaaaat tagcaaactt atttttgctt
    28561 actacactat taggtttttt tgacttttta ttttcatgtt gtaatttata tgactctaat
    28621 tgttcatatg tagttatatt tgcatccttc cactttttaa ggattccttt taagtatgat
    28681 aaattcatat tcattctttc agcacatatc tctatagctc ttttaaatac tcttatatct
    28741 acttccttag atacttctaa taaccattca gctgtgactg gatatactac tcctatattt
    28801 tcttcatata gcttcttaaa ttcttttaaa tagttatcca caggcttatc tatatacata
    28861 ctattaaaac tattaatatt aatactatta atattagtgt ccacattttc cgtgtccggc
    28921 tgagtcgtgt ccggaaaagt aggacatggt tccatgtcta tatttttagg acatggtttt
    28981 ttcttctttt tatttacttt tacacctctt ctagatatac attcatctat ataagttcta
    29041 tcaaatacta tttcatatat attattttgc attttacctt gtttagattt atttttatga
    29101 atctttatat atccactaag ttccaattca tttttgtatt ttgtaaatgt atcttttgat
    29161 atattcaatt cataacaaat taaatctcta gttggaaagc aagttccatt tatccctgca
    29221 aaactggata aataagaata tagcattcta gcaccaacag ttaaccatct atctcttgct
    29281 actaaccttg gcataagacc atacccatca cttaatatat ttcctttttc tataataact
    29341 ttgtccatat tattcaagag attcacctac ttaattatcc ctttttctct ttgcaatttc
    29401 tcatatccaa cacatatctg gtcatattcc tgtttactta aatctttaac ttctttatta
    29461 aacttataat acacttcact ttttactcta tctgaatctt tgcctattgt atttcctatt
    29521 gaaaacaatc tttttatttg attttgactt atttttattt gaccattttc acttgcttta
    29581 atagaggagt ttgttaagtc atttctttta taatcttctt ttccactagt agcatcaaaa
    29641 gtatcatttt ctgtaatgtt taataactga atgtacaaat accttgtctg atatgtttca
    29701 attcctccta atgcttgtaa ctcattagag ccttttaatt gtaattctct cattggagaa
    29761 gtaaatatta tctcttctgt tggtttctct ccattaatca atgttagagt tgcataatca
    29821 tttgtaaagg tcactatagg gcataattta gcttcttgta acagctctgt tgcttgaggt
    29881 aagaagtctg ctaactcaaa atatttaaag ttagcaaact tgttttgacc actctttttt
    29941 atatctaatt tgttaaactt aattcttaca tccatcaatt ttatgtaaat attattcact
    30001 tccatcaatt tctccctcca tttctctaat atttctttct aaaactgaca caaaaccatc
    30061 tatataatca ccatagttcc cttgtaattt gtattcttct aaattttctt tgaattgttg
    30121 taaagtgcat ccttttctat gtttatctat acaaaattct aatgctgata tttgtccaaa
    30181 ttttacatcc caatctactt catctactgt tgtaaatctt aataaaaata atctatcttc
    30241 taaatcctta attatttcat tttttgtttt catttattcc cccttatgtt ataatatacc
    30301 tataattttt gtaacttatt ttcacttaga gcctgtgcga aggctctttt tttatatctg
    30361 gacatctatt ggtctatctt tttcaagttc ttcttgatac atcatagctt ctctaaactc
    30421 aatagaagct tctaactcaa tgtcatgttc aagaccctct aatatatttt gactagcaaa
    30481 ttttacggct tcccaccaca ttaaactact attatttttt gtaccttgta atttacatat
    30541 ttctttttct gcttgtttaa tttgacctat tgctattagt ctagctgctt ccatttaaat
    30601 ccctcccaat ttattactat ctatataagc tacattctaa attgctacaa ttattacaca
    30661 ttaaaccttt tagaatgtta tattacctat tctaaatcca ttctttttgt gctataattt
    30721 gtttgtgtta tattttattt tttatttttt tgtgtgttgg ttatttgacc agcacttttt
    30781 tatttaatac tccaaccgat attttcttac cagttttaat atctttaaat actatgtctg
    30841 ctatagcttt tccatctttt ttaagagtta ctatattatt cttattagta ttaaggctta
    30901 acaatttcat cccccctctc tactgctttt aaaagttcat ccaaattttt accttgattt
    30961 ctttcaataa aatcatcaac ttcatatctt gaaatttttc taccatcacc tctagccagt
    31021 gattttatca aacctgtgct cactaaccta cgcataaaaa ctgtatctaa ttttaaaatt
    31081 ccccttgctt cttctactgt tatcaaataa tttggatagc ctctttttat taaaacaact
    31141 atatctttag gttctagtac ttttaccttt tgttcaacag tttgactctt aactctatct
    31201 gtttcttgtt tatttatctc tatttctatt aaacttttta aactatcact aaattgtttt
    31261 gctatacttt cagaaatatc catgtgaaaa ttctctcccc tctttttaat gaaatatata
    31321 aaattaattt tatatctacg cattattacc aaaatcctat atatattatt ttcagctcca
    31381 tacctcatgt tataattgac tcaagaagta ttcccacata cttccaatac ataagcaaag
    31441 gatggtgaaa atatgaataa ggacgagtta aataaaattt gcgacgaagt gtacaacaga
    31501 atactaaaaa gtgaatattc agatgaaaca cttcttagga ataaaattag tgaatttatt
    31561 tccctaaaag aaaaatctgc tagtgaaata agtaatacag agctttgtat gtttttttat
    31621 tttgagaata ttgcatttac taaaaactat gttaaagaag tcttatctga aattctttta
    31681 gataaataat cacttatttc atctattttt attggctttg ttttttcttc aagcaaagcc
    31741 aatcttttcc ctatatcaga ctctttcaga agataatcag taatatagtg taaatctttt
    31801 tctgtaatct ttgtatcctt ttcattcatt aaaatacacc tctcttttat atattttgaa
    31861 tctaattttc taagtactaa cccaacattg aagtttgata actatattct ttttctatat
    31921 ctggtaatat gttatttact ttcaataagt catataaaaa caatcttccc ttttgagtcc
    31981 acttagttgt catttttaca tcagtcattc catcacttct agttatgtct attgtttctg
    32041 aatgagtgta tcccttgcct tgatactgtt tatataaaag ccattgtcca ctttgtttgt
    32101 attgaatccc tctttcatga agtattttat tcatttcttt tccactcatt ccatagtctt
    32161 ttgctatttg agttatagtg acaagacctt tattttttaa tatcatatct gtataatctg
    32221 cctttggctt tagttctttt attacttggt ctttcatttt tccttctagt tgtaatttct
    32281 catttgcttc tacttgctct ataagatgtt gtaacgcttc tttatatgta gttggtaatt
    32341 tagtttgttc attttttaaa gctttttcca tttcatcaaa tttttttgta tatattcctg
    32401 taaatgctgt accttttatg cctgtcattt tatttgctat aaagtcacaa cctatctttg
    32461 ttattgcgta acatggtttc tttcttttat aatcatctaa ataagtattt tcaataaaat
    32521 aatcagatgg gtacaaatct gtactcatgt ttttatttgc ttcttccatt tggtttatat
    32581 aatttcttat atcccttagt aaaatcttgt gttctttttc aaccattaga gctatatccc
    32641 tactatcagt tgttagttta ttattaactt ttactatttt taaatctttc atatttacaa
    32701 actcctctct ataaaatgtc catcatttta ttgccatttt agcaactaat tctgaaaaaa
    32761 aatagtctgt tgatactcca tataattcag acaacttttt tatttcattt gctttgaact
    32821 catttttacc attaagttta agcctaaaac caaataaact aagacctaac aaatctgcaa
    32881 caaatttttg cgtatgttgg ttttctttca ttaacccttt caatctattg acatacattt
    32941 tttcaccctc taactttttg ctatataagc aagttcttgt ttatataata gcaacaaaaa
    33001 tgttaaaagt caatattttt gttgcttttt tggcaatttt aattgtaata acaaaaaaag
    33061 ttgctataat gtaaataaaa gttgctataa tgaaaactac aataaaggat gattttatat
    33121 gtttagatta aaagaactga gagaagaaaa aggaatatct ttagatagat taagtatgga
    33181 tttaaatgta aataaatcca ctctatctag gattgaaaat gggttaagag agccaaaaca
    33241 atcttttatt gaagactgtt caaattattt tgatgtatca acggattact tacttggtaa
    33301 aacagacata aaaaatccca atcaagtagt aaaatcttta aatcctattt tctcaaaaag
    33361 attaagagaa ttaataaagg aaaaaggtgt cacattaaat atattaggca aaaaattaga
    33421 aataagtact gatttattat ctaaatatga aaataatata atttcaccta aaagtgatga
    33481 tattataaag cttgctcatt attttgatgt tacaacagat tatcttttag gtacaacact
    33541 tgttagaaat catatagaca ctgtagccgc acacaaagca aatccacatg aggatttacc
    33601 tgaagaagct caagaacagc ttaatgatta tattgaattt ttaatgaata aatataagaa
    33661 ataggaggaa ctatgtcaga agaacaatat ttagaaaata gagtgaataa acaaataaat
    33721 tggtacagct ctaaaagtag taatattaaa aaaaaatttt ttactattca agttatagaa
    33781 ataattatag ctggaataat aactgcttta tcgaaatttt catttgaaaa tgaacatata
    33841 gcagatataa taactatttt aagtatagta gtaattattt tttcatcttt aagaaattta
    33901 tttaaatggc aagacttgtg gattaaatat agatatactg cagaattatt aaaaagagaa
    33961 aagtttttat ttctaactag gactaatatt tacataaatg aaaatgcctt caatttattt
    34021 acagaaagaa ttgaagacat tctaaataga gaaaatgaaa attggacaga actcaatttg
    34081 aactcttaat gatataaaaa actatacaat aaatgatgtt aaaggaactt catcatctac
    34141 agcttcatat gttttttcaa aaatatctgg cttacaagga tattgttctc ctcttacacc
    34201 agttataatc cagtcaccaa tagatgcttt taaaggacct tctaatgttt gtataataaa
    34261 ttctatatct gtctgataag cttctataac aactggcttt tttctaaact tttttacttg
    34321 cataaaagca cctccataaa tattattttg taaaatatta tatcacaaaa gaaaggataa
    34381 tatactatgc ctaaattata tgattataga gttttcataa gtcatgcttg gaaatatggt
    34441 aatgactatg atagattgat aaacttacta aataatgcta ataactttag ttattataat
    34501 tattctgcac caaaagaaaa acctttattt ccagatggaa caccttttag caattcagat
    34561 atagctaaaa aaataacaga taaaatatct ccttcacaaa taacaattgt tatatctgga
    34621 atgtatgttc aatataaaga ttggataaaa tacgaaattg atgaatcaat tagaattgga
    34681 aagccaatat taggaataag accttatgga aattctaata ttcctactta tgtttctgat
    34741 aatgctaata aaattgttaa ctggaataca gattctatag tatctgcaat tagagaactt
    34801 gtaaaataaa tattcttatg gagagtgcat ttattaaatt aactagtaac cctttactgg
    34861 gttactttaa agctctacat caaacatact ttcttttttt agggggattt caatgaataa
    34921 actagacgca cttttagact tagcaaataa tgaagagata gaaatttact acactgacaa
    34981 aatagcagat gacataaaag gattgtatat aaacagacaa ggactaaaga ttatatcatt
    35041 acttaattca ttaaaacaaa acaatgctaa actaatagaa atcttagcag aagaattagg
    35101 acatcatttt accagtgttg ggaactatgt atcttcaaaa aacagttaca aaaataaaat
    35161 cttgatagac aaaactgaaa acaaagcatt aaaatgggca tgtgaatttc ttataacaga
    35221 agaagaaata atacatgtta ttaattcaca cgctacaagt gtatacgaaa tagctgaaga
    35281 attacaagtt agcatcaact tcttactaaa aagattagaa tttctatcaa aaaagaaaag
    35341 catgttggac ttaggaaata atagattttt agtattaact aatttgccaa atttctacat
    35401 atatgaggat attttttaaa ctcatttatt ctacttttat agatttttta cttaataaat
    35461 atatatttca atattattat aataaactac acataaaagc taaaaaattg taagaatatt
    35521 aagaaaataa ttaagtaaaa accagataaa caaaattaag ataatattgt acataacaaa
    35581 agtatataaa gagcagttaa tctgctcttt tatataaaca ccaaacaaac atacattcta
    35641 aaagggaggg atactattat gaaaggtgga gtaagaaaaa gaagtaacaa atggtattac
    35701 tactttgacc taggcatagt agaaggaaaa agaaaaaaag tagaaagagt tggaggcaat
    35761 actaaaaaag aagccgaaaa agccttaaga gaagcactaa atgaatatga aaactctggc
    35821 atagtatttg aagaaagcaa tatcagttta tcagactact tagacttttg gtacaaagaa
    35881 tatgtcttac ttaactgtaa atacaacact caagaaagct accgaataaa catagaaaaa
    35941 catataaagc caaaactagg agcttacaaa gtaaaagctt taactcctgc aatactacaa
    36001 aacttcataa ataaaaagta caaagaggat tactctcaaa atacattaca agtattaaaa
    36061 gccatattac ataggtcatt aaaatcagca gtccatcctt acaaacacat acgagaaaac
    36121 cctatgcaat atgtaagcat accaaaaact aaatctaaaa cagaaactaa taaagttaaa
    36181 actattacat tagaagaatt taatcaaata ctaaatatat ttcctcaaga ttcatttcaa
    36241 cgtatagttt tactaattgg atttcatact ggtatgcgaa gaggtgaaat tattgcacta
    36301 aaatgggata atatagacct tgataataaa actatcacag taaagcatac tttgattaaa
    36361 aaaccaaatg gaatgtttga attagggcaa ccaaaaacag aaagctcttg cagaactata
    36421 tttacaggtg acactttaat aaaggcatta aaagaacata aattatatca aaagaaaatg
    36481 aaattaaaat atggagaatt ttactttgat agtgattggg tatgtaccaa agaaaatggt
    36541 caacaagtga atactcacac tttagacact atagtaagac aaattcgagt agctttaaac
    36601 aatgacttcc attttcattc tttaagacat gcacatgcta ctctattatt agaaaatggt
    36661 gctaacatta aagacataca aaaccgttta ggtcatagcc aactatcaac tacaatggat
    36721 acctattcac atgtaactga taaaatgaaa aatgaaactg tagatatatt tgaaaaaatt
    36781 acaaattaga gtttgccacc caaaaatata atacggtggc aaatgggtgg caaaatctaa
    36841 tttatctatt ttaaaagcta aaattatcaa atttatatag tcagctatac gcttgtaatt
    36901 tcaaggcttt agagtatata acaaccataa ctaatataag gtattaataa taaatctaat
    36961 aaataaaact taatatttac ttaagttgta atactattct aaattgtata attaaaattt
    37021 aataagttct taattatata gtcattgatt tatttgcaaa aataaaaaat gccaatctat
    37081 ctctaaacaa caaaaactga atactaataa aaatttaata aaaaatttta tcaatattca
    37141 gtataagttt tagttacata tcaaattttc caatgcatca ttaggtgcta caataggata
    37201 aacatcataa tccttatcta tatcacattg caaaaatact ggtttattaa aatctatcat
    37261 atctagcgcc tttcctaatt cttccatact ggatacctta tatccatcaa tgttatatgc
    37321 atttacgagt ttaacataat ccacattttc attaatgtca gtttgagagt atctttgatt
    37381 ggaaaataac ttttgccatt gccttaccat tcctaatgtt ctattattga gtagtaaaat
    37441 aagcataggt acattataat ttgcaactgt agctagttca ttacagttca ttctaaaact
    37501 tccatcacca gtgactaaaa ccacattttt atctacatta ccaactttag ttccaatagc
    37561 tgcacctaaa ccaaatccca tagttcctaa ccctgctgat gttataaaac ttttattacc
    37621 tttaaaattc cagtacttag cagcccacat ttgatgttga ccgacatcag ttactaccac
    37681 agtgggtttc tttaatgttt catatttttc atttattttt ttaagtatat tttgtggatg
    37741 aaattcatat gtttgtacac cctcactctt tctaaatctc tttatctctt ctttccaatt
    37801 gctattattc ttactttcaa ccctttcaat taataaacta aggactagct ttacatctcc
    37861 aaccaaagat acattagatt ctatattttt acttatttca gatgggtcta tgtctatgtg
    37921 tattattttt gcatttttag caaattcaga acttttactt atcactctat cactaaatct
    37981 cgcacctatc gctattacta aatctgaatt agaaagtgct aaattacttt ctctacttcc
    38041 atgcattcca accattccaa gagatagttc attctttcta tctatatttc caagccccat
    38101 aagtgtattt agtacgggtg tatctatctt agttgcaaat ttttcaagaa tttcctcact
    38161 gtctgaagat ttaacacctc cacctgcata tattacaggc tttttagatt cttttatgat
    38221 atctattgct tcatttaaaa gcttaatatt agtttcatca tctagatcaa aatcactctt
    38281 ataatccata taatcatcta tttgacataa atcatagtcc tcaccactaa aatccatttc
    38341 tgctaaaaat aaatcttttg gaacatctac aagtacaggt ccttttctac ctgaatttgc
    38401 aactctaaaa gcttctttta ttgtaggaac taattcttta gtgtttctaa ctaaataatt
    38461 atgttttgtc atagataatg ttgctccagt tatgtcaatt tcttgaaaag aatcttttcc
    38521 taataaacta gttggcacct gccctgaaat tacaaccata ggtgaagaat ccataaaagc
    38581 tgttgctatt ccagttatgg catttgttgc tcctggacca gatgttgtaa aacaaactcc
    38641 tactgtatta gtacttcttg cgtacccatc tgcagcatga actaaacctt gttcatggga
    38701 tgtccttata tgtttaaaat catctgaata atcgtataga gcatcataaa gaggtatcac
    38761 ggctccacca ggatatccaa atattgtgtc aattccctct ttctttaaac attctaaaat
    38821 tacctttgct ccattcatac gcactactat acaccactct ttccctcaaa ctaatccatt
    38881 taatatatat ttattttaag aatgacatca tacttcttag atttctacca acttcagcaa
    38941 tttctgtatt atattcttct tctctagttt ttaggaagtt ttcacgtcct tcttcatttt
    39001 ctttaatcca cgcttttgca aattttccat tttgtatatc ctctaaaaca ttttgcatac
    39061 cttgttttgc agcatcagtt attactcttt taccagatac ataatctcca tattctgctg
    39121 tgtcacttat actatatctc attctttcaa atccaccttc atatataagg tcgactatta
    39181 gtttcatttc atgtaaacat tcaaaatatg ctacttcttt ttgataacct gcatctacaa
    39241 gcgttttata tcccaatttt ataagttcac ttattccacc acaaagtact gattgttctc
    39301 caaataaatc tgtttctgtt tcttctttaa aagtagtttc taaaacacct gctcttgttg
    39361 ctcctatccc ttttgcatat gctaaaactg tctctgtagc ttttttagtg tggtcttgat
    39421 atactgcaaa tagtgcagga actccacttc cctttgtaaa tacatttcta actaagtgac
    39481 caggtccttt aggagctacc attacaacat caacaaattc tggtggttgt acttgattat
    39541 aatgtatatt gaatccatga gcaaaagcta atgtctgacc ttcttttaag ttatccttta
    39601 cactctcttc ataaactttc ttttgttttt catcaggcat taacatcatt gtcaaatcac
    39661 tttcttttgt agcttctgct acactcttaa cttcaaatcc atcttctttt gctttttgtg
    39721 ctgatttact tccgtcataa agacctatca ttacatgtac accgttatct ctaagatttt
    39781 gtgcatgtgc atgtccttga cttccataac ctaaaactgc aaccttttta ttttttaaaa
    39841 cctctaaatc cacatctttt tcataataca ttcttgccat tttcatttcc tcccaatact
    39901 ttttcttatt tttatgatat ttatttttat tagaaattca tactcattat cattgtttta
    39961 atattattca acagatttaa attctaaatt tatataatct tacaattata attttagaat
    40021 tttcagtttt tattagctac aacaaactaa taaatttatt aactgtga
//
LOCUS       DAMJYC010000028        39790 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJYC010000028 DAMJYC010000000
VERSION     DAMJYC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.029"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            416..2494
                     /locus_tag="PC472_001631"
     CDS             416..2494
                     /locus_tag="PC472_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2726958.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2494..3273
                     /locus_tag="PC472_001632"
     CDS             2494..3273
                     /locus_tag="PC472_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2726959.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3266..4075
                     /locus_tag="PC472_001633"
     CDS             3266..4075
                     /locus_tag="PC472_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2726960.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4095..4577
                     /locus_tag="PC472_001634"
     CDS             4095..4577
                     /locus_tag="PC472_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726961.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4598..5002
                     /locus_tag="PC472_001635"
     CDS             4598..5002
                     /locus_tag="PC472_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2726962.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5158..6318)
                     /locus_tag="PC472_001636"
     CDS             complement(5158..6318)
                     /locus_tag="PC472_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2726963.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6526..8076
                     /gene="xylB"
                     /locus_tag="PC472_001637"
     CDS             6526..8076
                     /gene="xylB"
                     /locus_tag="PC472_001637"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2726964.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            8172..9509
                     /gene="xylA"
                     /locus_tag="PC472_001638"
     CDS             8172..9509
                     /gene="xylA"
                     /locus_tag="PC472_001638"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2726965.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNISRINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            10031..11230
                     /locus_tag="PC472_001639"
     CDS             10031..11230
                     /locus_tag="PC472_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2726966.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNGWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            11397..12182
                     /locus_tag="PC472_001640"
     CDS             11397..12182
                     /locus_tag="PC472_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2726967.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            12351..13916
                     /locus_tag="PC472_001641"
     CDS             12351..13916
                     /locus_tag="PC472_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2726968.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            13930..15261
                     /locus_tag="PC472_001642"
     CDS             13930..15261
                     /locus_tag="PC472_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2726969.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            15275..15763
                     /locus_tag="PC472_001643"
     CDS             15275..15763
                     /locus_tag="PC472_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726970.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIRQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            15777..16244
                     /locus_tag="PC472_001644"
     CDS             15777..16244
                     /locus_tag="PC472_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2726971.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            16247..17398
                     /locus_tag="PC472_001645"
     CDS             16247..17398
                     /locus_tag="PC472_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2726972.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINLLLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYGLIVLLNYLQ"
     gene            17718..18470
                     /locus_tag="PC472_001646"
     CDS             17718..18470
                     /locus_tag="PC472_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2726973.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            18471..20378
                     /locus_tag="PC472_001647"
     CDS             18471..20378
                     /locus_tag="PC472_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2726974.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NMLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVMIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIISITVIGYGYY
                     LSTNIRTGNITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYDERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            20530..21210
                     /locus_tag="PC472_001648"
     CDS             20530..21210
                     /locus_tag="PC472_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2726975.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            21204..22223
                     /locus_tag="PC472_001649"
     CDS             21204..22223
                     /locus_tag="PC472_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2726976.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVNGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            22654..23814
                     /locus_tag="PC472_001650"
     CDS             22654..23814
                     /locus_tag="PC472_001650"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2726977.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILSKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            23816..24214
                     /locus_tag="PC472_001651"
     CDS             23816..24214
                     /locus_tag="PC472_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726978.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            24475..24783
                     /locus_tag="PC472_001652"
     CDS             24475..24783
                     /locus_tag="PC472_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2726979.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            24872..26218
                     /locus_tag="PC472_001653"
     CDS             24872..26218
                     /locus_tag="PC472_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2726980.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            26451..27548
                     /locus_tag="PC472_001654"
     CDS             26451..27548
                     /locus_tag="PC472_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2726981.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            27647..28561
                     /gene="murQ"
                     /locus_tag="PC472_001655"
     CDS             27647..28561
                     /gene="murQ"
                     /locus_tag="PC472_001655"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2726982.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            28671..29591
                     /locus_tag="PC472_001656"
     CDS             28671..29591
                     /locus_tag="PC472_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2726983.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            29608..30459
                     /locus_tag="PC472_001657"
     CDS             29608..30459
                     /locus_tag="PC472_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2726984.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            31070..32131
                     /locus_tag="PC472_001658"
     CDS             31070..32131
                     /locus_tag="PC472_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2726985.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(32247..33221)
                     /locus_tag="PC472_001659"
     CDS             complement(32247..33221)
                     /locus_tag="PC472_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726986.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            33677..34558
                     /locus_tag="PC472_001660"
     CDS             33677..34558
                     /locus_tag="PC472_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2726987.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNEND"
     gene            34940..35743
                     /locus_tag="PC472_001661"
     CDS             34940..35743
                     /locus_tag="PC472_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726988.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            35755..36885
                     /locus_tag="PC472_001662"
     CDS             35755..36885
                     /locus_tag="PC472_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2726989.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISRSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            36889..38298
                     /locus_tag="PC472_001663"
     CDS             36889..38298
                     /locus_tag="PC472_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726990.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            38509..39018
                     /locus_tag="PC472_001664"
     CDS             38509..39018
                     /locus_tag="PC472_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2726991.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT        15574 a         4143 c         6990 g        13083 t
ORIGIN      
        1 cataaattat ttataattat aaaatccatt gttttgaaca atatctaata tattattatc
       61 tgtatttata aggtcactat acatattttc aagtccgata ttatctacat aataggcaaa
      121 cgttattcct gtatgtgttt taaaaaaact agatgtatat aggtatcatc acagatatta
      181 aaagtataaa taaaagatat taagaacgac tttcaagcaa catattagaa aattttacta
      241 aaatccggaa agcgattgac aaacaaagtc aaataaagta tattattggt aagttagttt
      301 aatgtacaaa tgaattatat ataaaaccta atttataaat aacgatacta aacgacatta
      361 tttaacttaa gctacacaaa gatagaatat tataccattg atttaggagg tagccgtgat
      421 aaaaatatct gaagactata ttgaaaaaag atttgacaat gaactattgc gaatagaagc
      481 atggggaaaa aactctttac gtattaggtc gtttgtagac caaaattttg ttgatgaaaa
      541 ctatgctctt aacgaaaaac ctaagttaaa taaagatgat attacaatta ataaaaatga
      601 agatggaagt gcaatcatta aaaatggaaa gataaaagct gtcttagacc atagagatag
      661 aattactttt tataatgata aaaatgaaat attattgaaa gaatatattc gattaagagc
      721 tgtaaaacat gatgatggtg gagaagatgt agggacaatt gagattacca aagactttaa
      781 ctctactcta aagttaaaat caagagaata tcgaccaagc tatagtggag aatttgaagt
      841 aactactaga tttgaatctg agccctctga aaagattttt ggaatgggac aatatcaaca
      901 taaattttta gatttaaaga atactgtttt ggagttagcc caaagaaatt ctcaaatatc
      961 agttcctttt tatatttcta gtctaggtta tggactttta tggaataatc cgggaattgg
     1021 aaaagtatct tttgctaaaa atatgacaga atggaagatg ttttctacaa attttataga
     1081 ttattggatt acttgtggag aaagcccaaa agagttaaat aaaaattatt ctcaagttac
     1141 tggaactgtt cctatgatgc ctgaaaacct tttaggacta tggcaaagta aactaagata
     1201 tagaacttca gaagaagttt tagatgttgt gaaggaatat tctaaaagag gaattaagct
     1261 ttcatcaatt gcaattgact attttcattg gcctaagcaa ggtgaatata aatttgactt
     1321 agattattgg aaaaatccta aagaattagt taaaaaattg aaagaagagt attcagtaga
     1381 accaatagta tctgtatggc ctacagttca aagtgatgca gaaaactaca atgattattt
     1441 agaaaatggc tatttagtca atgttaatcg tggtgttaga atgacaatgc aaattcaagg
     1501 aaatacagtc tttgtagata tgacaaatga aaatgcaaga gaatatgttt gggatagaat
     1561 agataaaaat tataaacaac ttgggattga ctattattgg ttagatgtag ctgaacctgg
     1621 ctattctgtg tatgattttg ataactatag atataaaaaa ggaaatgttt taagttgtgg
     1681 taatatttat cctattgatt atttaaaaat gatttatgat ggtttacata atgacataga
     1741 gtctgttgta actcttgtaa gaggagcttg ggcaggagct caaaagtatg gagctttagt
     1801 atggtctggt gacattgatt ctagttttga agcttttaat aatcaagtaa atacagggtt
     1861 aaatatggga ctggcaggta taccatggtg gactactgat attggaggtt tccatggagg
     1921 aaatcctaaa gacccagaat ttagagaact tatggtaaga tggttccaat atgctacttt
     1981 ttcaccaatt ttgagaatgc atggagatag attgcctcat agtaaaccat tatcaaataa
     2041 aggtggaggc tcaatggtaa ctggagctcc taatgaaata tggtcttatg gtgaagaagt
     2101 tgaagtaatt cttacaaaat tcataaaaat tagagagagc ttaaaaactt atttaaaaaa
     2161 actgatgaaa gaagcgcatg aagatggaac accagttatg agaactttat tttatgaatt
     2221 tcctgaagat gataaaactt gggaggtgga taatacatat atgcttggag atgaaatttt
     2281 agtagctcca ataatgaatt ataaagatag aagtagaaaa gtatatttac caaaaggaca
     2341 tacatgggaa aatatattta gtggagttag ttatgaaggt gggaaaactt atgaagttga
     2401 atgcccactg gaagaaattc ctattttcct aaagcaagat tccagttata attttaaaga
     2461 actaaaaaat attttgggag aggtgaaatc ataatggagc cacaattatg gcaattatta
     2521 ttaatcgtac tatatggatt ttttataaac tatgaaaaaa attcaacaat gtttggaaca
     2581 tatcaaccag taacagcagg ttttattact ggtcttatct taggagatat aaatacaggg
     2641 ttatatattg gaggtacatt acagctatta tcattaggta ttagtaactt tggtggagca
     2701 tcaatacctg attatcaaac agcaagtata gtagccacat ttataactat tacgactaag
     2761 caagaagcat ctgtaggaat ctctataggt attccagttg ctttattgat ggtacaatta
     2821 gatgttctta gaaatactat tggtatatgg ctagttcata aagcagaaga tggagctaaa
     2881 aaaggaaatt ataaaaatat aacctatatg caaatgttag gagtactatt aacagcagct
     2941 acaacaggta ttccagttgc tctatctgta atatttggac cttctttaat taataccatt
     3001 ttgaaatata cacctgaatg gttaacaggt ggtttaacag tagctggagg actacttcca
     3061 gcagtaggga taggattatt acttagatat cttcctgcta aggagtattt tagttactta
     3121 gtaattggtt ttgttttagc agtatatatg aaagtaccat tattaggtgt agctttaatt
     3181 ggtggagcaa ttgcacttat aatttacaaa aagaatttgg aaaatcaaga acaacaatat
     3241 actgtagtag gaggtatgga cgaagatgag tgataaggta tcattaaata aatcaaatga
     3301 aaaggtgaaa aaaagtgtct tatggcgctg gttttttact agttctgttt cttcaaatta
     3361 tgagaagatg caggccttgg catactgtta tgcagtacta ccttttttaa aagtagtata
     3421 caaagataat ccagaaaagt tacaaagtgc tgtattaaat catttacaat tttttaacac
     3481 taatccttgg gttgcccctt atattttggg tattaatgta gcaatggagg agttatcaag
     3541 tgaagagact gaagaggctg ttgcttcaat taaaacaggc ttgatgggac cagtagcagg
     3601 tttaggagat agtttatttg tcgttattcc gtggacaatt tttggagcta tagcagctaa
     3661 tatggcgata gatggaagtc catttggaat cttactgtgg attgtagtga gtgtagcatt
     3721 gaaattatta agtataccac tatttaatgc tggttataca tcaggtacta agctaatatc
     3781 ttcaattgaa aaaagtttaa agattttaac tgagagtaca tctattttag gtttaatggt
     3841 tgtaggtgca ttaatacctt cagttgtaaa agctaatgtt gctttgaact ttcaacaagg
     3901 tgattttaca atgaaaggtc aagaaatatt agaccaaatt atgcctgggt tagttcctgc
     3961 aattctagtt gtgattgttt attgggctct taaaaagaat ataaaaccta tatacttaat
     4021 tttaggagta atggttgttt caataatttt atatgcatta ggtattttaa aataaggaaa
     4081 ggatgatatt agatatggaa atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa
     4141 tccatggtca agttgcaact atgtggagta ataaattggg tgttacaaga ttaatggtag
     4201 taaatgatgc agtagctaac aattctgttc aaaaacaagt tttaagaatg gctacacctg
     4261 caggtatagc gtcatctata attacagaag agactgctat aaaaaatatt actgcaggaa
     4321 agtatgaagg acaaaatgta ttattaattg ttaagtctcc tgtggacctt attccatttg
     4381 tagaggcagg atttaaaata cataatataa atgtaggaaa tatgtcaagt agaaaagata
     4441 caacagttct aagacctaat atcagtgtta ctgaagaaga gaaagaagca tttaaaagac
     4501 ttttagatga aggaattgaa attacaacta taatgacgcc tgatgataag aaaacttatc
     4561 ttgtagacat cttatagatt taaagaggtg tattaaattg aatagtatta ttttgatgag
     4621 tcatggcaat atggctgaag aagtcttaaa ttctgcaaaa atgataatag gggattctat
     4681 agattatcct actgtaaata tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt
     4741 aaaaaaagct ttagaagtgt acaaggaaag ttcagaaata attataatgg cagatttact
     4801 aggtggaact ccatgcaatg ctgctttatt agtagccaat aatgacagtc gaattaaaat
     4861 tatttcaggg ctaaatttag gtatggttat tgaatcagct tttttagata caaaggaatt
     4921 ggcagcaaaa ttagagaaaa taggtaaaga taatatttct atggttgaac aaaatgcaag
     4981 tttattatct gatgatgagt aggaagatta tattaatatt taaaacgaaa tacttatgtt
     5041 aatacttaat tgatatacaa tttttaaaaa atatagctaa tcttgcataa actatataat
     5101 aaatctaatt ttagaatgta taatttaaag ggttatggct gtatatagtc ataaccctta
     5161 tatttcatta attaaagtta aattttgtat attcaaaaag ctcttgatat ttacggcaga
     5221 gccaccatat agagatgcct cagaccctaa tgatgaaatt tctatattta ttcctttgtt
     5281 aaaagagcta actaacatat cttttgtaat ctgtaatatc tcagggatat cactaattat
     5341 ttggctattt aaatatatta tttcaggagc ataagttgta atagcattat ttatagctat
     5401 tgttaagtaa ctacaaaact catgaataac ttttttggca ttctggttat cttcataata
     5461 tagttgcttt actatatcag aatctatttt aggaatattt tctaaagaag aaagctgttc
     5521 aaataccttt ttttctgaac aatactgttc taaacagcca cgatttccac aaggacatag
     5581 tttaccatta ggcatgatga tagtgtgacc aatttctcca ctcataccat ttctgccact
     5641 atataattta ttatttatta taatgcctga accaaatcca ctgtgaatac tgagactaag
     5701 tagtgagtta tgtacagttg aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt
     5761 ttctatgtga ataggaaagt catacttttt gctaaggatg ctatataagt caatctcatt
     5821 aagattgtaa tatggagtaa acaatacttt attttcacaa gtaatcccat gtatagccaa
     5881 agtaagacca attaccttat aaggagtgtc tattttggat atgttataac tatttataat
     5941 ttcatctatc aattgtataa cattgtcttt acttacttgt atatctgtta atttcttaga
     6001 atttataata gttccatcta agtaagatag agaagaaaat atatagtcgt atccaatgtc
     6061 catacttaaa gaaatccctg cacatttatt aaatactaat aatatgggtt ttcttccgcc
     6121 actatgagta ctatttccaa ttccgatttc atgaactaga gattcatcaa taagtttttt
     6181 ggtaatagcg gatatagttg ctttattcaa tcctatagta gaagctatac ttgccctaga
     6241 aataggacca ttttttataa tttgttcaag taccaatctt tcattcattt ctcgaatagt
     6301 gtatttatca gtaaccaatt tgatattcct ccttaaaata atattgtaat actttttaca
     6361 caaaaataaa aggttatttt gcattgacaa agataattaa atattttatt attagttcat
     6421 aagttagttt aatatactaa caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa
     6481 attaggatag gaaaacgata gttatgaagt ggcattcaag gagggatgaa gttggagtat
     6541 gtattaggtg tggatattgg tacatcaggt acgaaaacag ttttatttga taaactagga
     6601 aatactataa aaagttgtac atatgaatat cctcttatac aagagaaatc aggttgggca
     6661 gagcaggatg caaatgattg gtggaaagct gtagtcgaat ctataagaga agtagttcaa
     6721 agttcaaata taagcagtga atgtataaaa ggaattggat tatcaggaca gatgcatggt
     6781 ctagttatgc tggacaatga aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa
     6841 cgaacagtaa aagagtgtga ggagataact gatttagtag gtgaagaaag actgattgaa
     6901 ataactgcta atccagcact tacaggattt acagcatcaa agatattatg ggttagaaac
     6961 aatgaaccag atatatataa aaatactaat aaaatacttc ttccaaaaga ttatataaga
     7021 tacaaattga caggtgaata tgctacagag gtatcagatg caagtggtat gcagttgctc
     7081 gatataagaa aaagagattg gagcgatgaa gtactagaaa agttaaatat agataaaaat
     7141 cttttaggta aagtttatga atcacaagag attacaggtt atgtgactag agatgtagca
     7201 agtttgacag ggcttaaaga aggaacaata gtggtaggag gagctggaga ccaagcagca
     7261 ggagctatag gaaatggaat agttaaagat ggagtagttt catctactat aggtacatca
     7321 ggtgtagtat ttgcttatac aaaagagcca aaaattgata aagaaggtag agttcatact
     7381 ttctgtcatg caataccaaa tacatggcat gttatgggag ttactcaagg agctggcttg
     7441 tcattaaaat ggtttaaaga taatttctgt caaagtgaag tggaagtttc aaattcttta
     7501 ggtgaagatg tatatgagat tataaataat caagtaagtc aagtaccaac aggatgtaat
     7561 ggattgcttt acttaccata tatgatggga gaaagaactc ctcatcttga cccttatgca
     7621 agaggtgtgt tctttggatt atctccaatc cattctaaaa aagaaattgc aaggtcaata
     7681 atggaaggtg tttcatatag tcttaaagac tgtatggata taatagagaa tcttaatata
     7741 gacgttaatg aagtaagagc ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg
     7801 caagcagata tgtttaatca agatgtatat actataaatt caagtgaagg accagcactt
     7861 ggagttgcaa ttcttgcatt agtaggagct ggaatatatg aaaatataca aaaagcatgt
     7921 gatgctataa taaaaacttc aactaaatta gaaccaataa gtgaaaatgt agatacatac
     7981 aatatatatc atagattgta taaaagaata tacaaatctt tgaaagatga ctttaagtta
     8041 ttagatgaag tagttaatgc aaaaataaat aattaaatta gcttaaaaag aaaaataaag
     8101 atagaaaata gttttaaatt aagaaaacat actacatata ttatatgtgt aaaatattag
     8161 gaggacatga aatgagtgaa atatttaaag gaataggaca gataaaattt gaaggagtaa
     8221 aatctgacaa tgaattagca tttagatact acaatcctga acaagtggta ggtaataaga
     8281 ctatgaaaga acacttaaga tttgcaatga gctattggca tactttatgt ggagagggta
     8341 atgacccatt tggagtaggg acagtagaga gaccttggaa taatataaca gacccaattg
     8401 agatagcaaa gattaaagtt gatgcaggat ttgaatttat gtctaagatg ggaattgagt
     8461 atttctgttt tcatgacaga gatatagcac cagaaggaag agatttagaa gagacaaata
     8521 agatattaga tgaaatagtt gagtatataa aagttaatat ggaaaagaca ggtataaagt
     8581 tattatgggg aactgccaat atgtttggaa atccaagatt tgttcatggt gcatctacaa
     8641 catgtaatgc agatgtatat gcatatgcag cagctcaggt taaaaaagct atggaaataa
     8701 ctaagtactt aggaggagaa aattttgtat tctggggtgg aagagaaggt tatgaaactt
     8761 tacttaacac taatacagaa cttgaaatgg ataactttgc aagattctta caaatggctg
     8821 tagattatgc taaggaaata ggattcactg gacaattctt aatagagcct aagccaaaag
     8881 agcctactaa acatcaatac gattttgata ctgctactgt tttaggattt ttaagaaagt
     8941 ataatctgga taaatacttc aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac
     9001 atactttcca acatgaatta aatatatcaa gaataaacaa tgttttaggt agtatagatg
     9061 caaatcaagg agacctatta ttaggttggg atacagacca atttccaaca aatatatatg
     9121 atgcaacact tgctatgtat gaagtgttaa agcaaggtgg aatagcacca ggaggattta
     9181 actttgactc taaagtaaga agagcatctt ttgaggtaga agacttattc ctagcttata
     9241 ttgcaggaat ggatacattt gcaaagggat tattaatagc tcataaatta ttagaagatg
     9301 aggtatttga aaactttact aaagaaagat atgcaagttt tagtgaaggt ataggtaagg
     9361 atatagtaga aggtaaggtt ggatttaaag agttagaatc ttatgcactg caaatgccag
     9421 ttataaagaa taagtctgga agacaagaaa tgctagaagc tatacttaac agatatatat
     9481 atgaagtaga tacaatttct aataagtaat tgtgtagtaa ttaaacttgg atgtttaaat
     9541 aaaataacag gatatgttct tgtttttatt ctaaaacgga caaagataag tatttgtaaa
     9601 aattctcata atcatgataa attaaggata tagacttaag tataattagt ttatatcctt
     9661 aaaatttcta taaaaaataa tcatttctat agttatcaat atttttaatt atcaatatat
     9721 ttctcaacta ataaataaaa atcagtaaag tatataaatt taagtcatta taatggataa
     9781 attttttagc atatcttttt tagtatatca ctgataagaa tttactttca ggatatagca
     9841 gtaaaataga tatttgaata taattgttat tttataactt tcatgaaata attttgaaaa
     9901 tacaaataaa aatttttaaa tagaataata gtggaatata tcttcaagaa tttcttttaa
     9961 ctgtttaggt gataataaaa taatgataca atgtattaaa atataaaatc tgaaagtgga
    10021 tgagaattac atgaaaaaga aagtattatt tttagtaatt attttagcat ttataatctt
    10081 attaggtaat tctacttatt ctcaaaagaa tcagttgtct gaagaggaaa aggtggatga
    10141 ctttaattat gtgtataatg ttgtaaagac aggatatcca tatctaggtg taaataagag
    10201 gcttaataat attgattggt tagcaaataa ggatgaatat acaaaacgaa taaaaaacac
    10261 tgaaaatgat gaagaattta tagaagaact gtcatctata ttatctgatt taaataataa
    10321 acacacagaa gtaatagata ataagaaaag atatgaactt tttaagaaat catactctaa
    10381 taatggttgg tatgattttc ttaatgataa aaaagttgtg gatagatatg actctatgaa
    10441 tcttaaaata aaaatgccag atgatatttt tgtaaaaaaa gaattaattt taaaggatgt
    10501 tattaagggt cagattggat atatgtattt accatcaatg tcatcaaaga atggctctat
    10561 aaataatgat ttaaaaataa ttggagatta tattaacact ttagaaaaac ataaagcttt
    10621 aattattgat ataagaggta atttaggtgg aagtgatagt tattggcagg gaatcgtatc
    10681 taaactcata aaaaatgatg tcaaaataaa tggttataga atttatagaa ataataatga
    10741 aatagtaaaa aaatatacaa atgcaagaaa tataaaatta aatcctgtag aaaatttacc
    10801 agcaagtgta aaggaaaatg cacctaaaga aattataaaa gattttaatg attttgaaga
    10861 tacttcctat actataaaat ctacagatga ttcaaaattt aagggtaata tatacttact
    10921 tgttgataga aaaatatatt catcatcaga gtcatttgca atgttttgta aagaaaataa
    10981 atttgcgact ttagttggtg aaactactgg cggagatgga gggtgtatag accctgtatt
    11041 gtttaattta aaaaatagtg gtctaatagt tagaatggca agttgtatgt atttaaataa
    11101 gaatggaata tgtgatgagg aatttaaaac tattccagaa tttaaagtaa aagattgtaa
    11161 aaggactgct gattttaaag atgataattg tataagaaaa gcactagaac ttgaaaatat
    11221 taattattag ataaaagaat aaaattaaaa ctacattttt ttaaacatca attatagcag
    11281 tattttttat agtcaatttt atgggctaaa tagtaaatat aagaatacgc tgtaatttaa
    11341 tgttgaatat tggtataata tttatgacga tttttaatta aagggggtaa tagtttatga
    11401 gattagaaga aatgattttt ttaaattata ataaattgaa tgatacagat ttattaatat
    11461 ggaaatatat aactgcaaat aaaaaacatt gtagtaagat gtcaataaat gaacttgcta
    11521 tgaaatgtaa tgtgtctcgt gcaactatta caagatttgt taaaaaatta gatttaaaag
    11581 gtttcagtga gtttaaagta ttattaagct gggaatcaga aaaggttcat gtcatagaaa
    11641 atcagtcatt tgatattgca tgtaattcaa taattaagta tgttgaagat caaaaaaata
    11701 aagattatga tgcattgtgt aaacttcttt atgaatcaag aacaatatac gcttatggtt
    11761 caggtgatat tcaaaatgtg gttgcaaaac agataaagag aatgttctta tcatgtcaag
    11821 aaataatata tgattttgag ggaagaacat ttgatgattc tttttacaat attgtcagta
    11881 aaaatgatgt tatgattatt atatctctaa gtggtgagaa tgagaataca cttaatattg
    11941 ctagaagatt aaaattaata ggaactaaaa taatttcaat tacagagttt aaaaataata
    12001 cattgactga tttaagtgat gaatcaattt atatttcagc gactaatatc tcatttttgc
    12061 aaatgcaccc tagttataaa ttgacaatgt tatattttat attgattgag ttattattta
    12121 ttaaatatag tatttataaa aggcatagaa tgattacaga agggattgat tgcaaattgt
    12181 agtcattctt ttttttatga gaatttgaac ataaaaatgt gcaaaaactt atttgactgt
    12241 gaataagctc cgaatgtacc ctaaagattg tatatattta ttaacctgtt aaaataatag
    12301 atatctaagg ccaaagataa actaaaaata tgaggagggg agtaatttat atgatgaaga
    12361 aaatacaacg ttttggaggg gcaatgttta caccaacact attatttgct tttgctggga
    12421 taatggttgg attctcaatt gtatttcaaa atcagtcaat tatgggttca cttgctacac
    12481 cagaaaatat ttggtaccaa ttttggggtg taatatctag tggagcttgg atggtattta
    12541 atcaattacc attattattt gctatttctt taccaatagc acttgcaaaa aagcaacaag
    12601 caagagcttg tatggaggct ttagcaacgt atttgacttt taattatttt gtaggttcta
    12661 tgttaagttt ttggggaaaa agctttggag ttgatttcgc agctgaaata agtgctggaa
    12721 gtggacttgt aagcattgct ggtattaaaa cattagatac tggtatggtt ggtgctttgt
    12781 taatatcagg tattgtcatt tatattcata atagatttta tgataaagaa ctaccagatt
    12841 ttattggatt atttagaggc tcatcacttg tagttgcaat ttgtttcttt gttatgatac
    12901 cagttgcatt attaacttgt tttatttggc ctcatattca aaatgttatt agacatttgc
    12961 aaacgttttt tataaatagt ggaaatatag gagtttggtg ttatgcattc ttacaaaaga
    13021 ttttaatacc aactggatta catcattttg tatatgcacc aatttgttat gatagtgttg
    13081 ttgttccagg tggtacatca gtatattggg ctactcatat tcaggacttt caaacttcag
    13141 ctaagactct aaaggaaatg tatccaatcg gattttcatt atccggatta tcgaaggtgt
    13201 ttggttcatt aggtgtattt ggtgctttct atgtaactgc aaagccagaa aaaaagaaaa
    13261 aagtattagg tttaatgatt cctgctactc ttactgcagt attaactgga attactgagc
    13321 cgttagaatt tacattttta tttgtagcac cacttttatt cctagtacat gcatttttag
    13381 atgcgtgctt gcaaacgatt tcgtttgcct taggagttgt tggtgatttt ggtggaggaa
    13441 ttattaactg ggttgtatta aattggcttc ctcttggaat gtatcactgg aaggtatatg
    13501 ttgttcaagt tgttgtaggg attatatttt catttatctg gttctttgta tttactttct
    13561 taattaagaa gtttgatatg aaaactcctg gtagagaaga agatagtgaa gaaacaaaac
    13621 tatatactaa aaatgaatac ttagaaacta aagatgaaaa aggaaataaa ttgagtaaag
    13681 cctctcaaca agcatctgaa tacataaaat tagttggtgg agcagaaaat gttgtagatg
    13741 ttacaaactg tgctacgagg ttgagattaa ccttgaaaga tgactcaatt atttctaaag
    13801 aagaagattt taaggcagta ggtgcacatg gtttagtgca taatggaaaa gctattcaga
    13861 ttattattgg gctttcagta ccaagtgtaa gagaagaatt tgaaaattta ttataaagga
    13921 gaaggaaaga tggaaagaaa aagacagagg atactaattg ctggtggggg tagtacttat
    13981 actgctggaa ttgtaacaat gttaattgaa agtgttgcta aatttcctat tgaatcaatt
    14041 aaattatatg ataataatga tgagagacaa agaaaagttg ctgaagcatg tgcaataatt
    14101 gttagagaga aaaatccaga aattaaattt agttatacaa caaatccaga agaagcattt
    14161 actgatattg attttgtaat ggcacaaatt cgtgttggtt tatatgcttt acgtgaacaa
    14221 gatgaaaaaa ttccactaaa atatggggtt gttggtcaag aaacttgtgg ggcaggtgga
    14281 attgcctatg gattaagaac aataggtcca ataatcgaaa tgattgatta tatggaaaaa
    14341 tattctccaa atgcttggat gcttaattat tcaaatccag cagctatagt tgcagaagca
    14401 tgtcgtgtat taagaccaaa ttcaagaatt attaatattt gtgatatgcc agtatgttta
    14461 gaggaaatat ttgctagagt tttaggttta aattctcgta aagactttga tgttagatat
    14521 tatggattga atcatttcgg gtggtggaca agtattaagg acaaagaagg aaatgattta
    14581 atgcctaaat tacaagaata ttgtgctaaa aaaggatatg aggaatttac acctcaaggt
    14641 caacacaaag aatctagttg gttagaaact atgagagcag caaaggattt attggaaata
    14701 gaaccgacta cattacctaa tacttattta aaatattatt taatggctga tgaaactgtt
    14761 gaacatgcta atccaaatta tacaagagca aatgaaatta tggatagaag agaaaaggat
    14821 acattcgaag aatgtgaaag aattataaaa aatggcacag caagagatac atggtttgat
    14881 gcaagtgaac attctcaatt tattgttgaa ttagcttgtg cattagcatt taatactcag
    14941 gaaagatttt tactgattgt tcctaataat ggagctattg aaaattttgc agatgatgca
    15001 atggttgaaa ttccttgcct tgttggaaaa gatatggttg aacctatgtc tataggtaaa
    15061 attccaactt tccaaaaagg tttaatggaa caacaagttg cttctgaaaa attagctgta
    15121 gaagcatgga tagaaaaaag ttatcaaaaa ttatggcagt cgtttactat gagtaaaact
    15181 gttccaagtg caaaagtagc aaaagaaatc ttagatgaaa tgattgtggc taataaggat
    15241 ttctggccaa cttttaaata aggagaacaa aaacatgata acaaataaaa taaaaaatat
    15301 cacagcagta ttaaaagatg aaaatatatt tctttcagtg gttatggttt gttttctaat
    15361 attacctgtt ttatatatag tgtatttaga attgacatca ttatttacaa atatttcatt
    15421 ttcacaactt ttaaacaatg atgttaatgc aagtttaaat ttaatctcta actttagtgc
    15481 agtatacagt gcatatgtta tttacaatat aaaaaagaat aaagagataa aagaaaacat
    15541 gattgctttc tatttagtct tattagggca gatattccaa atttacacaa taactattgt
    15601 tatcatgttt acctatattt ataaatttgt tggaattaga caaatcataa gcagtattaa
    15661 agaactacaa tggggaaaga gctataaggc tcttttttcc tcaattgcag tatgcttaat
    15721 atcgttattt gtaatgataa ttagaataaa gttaattatg tagaggagtg tctataatgt
    15781 tctttaaaag aaaaaaagaa gcaattacaa ttaattcata cattgatgga aaaataatta
    15841 atcttggaga aattcctgat aaagtatttt caactaaggt aatgggagat ggatttgcta
    15901 tgatagcaaa ggacaatatt cttaaatcac cgactaaagg agtaataagt tttattgctc
    15961 aaactaacca tgctatagga attaaattgc aaaatgatgt tcaaattctt attcatattg
    16021 gattggatag tgcaacaaca aaaaaagaaa actttaaatt agaagttaaa caaggtcaag
    16081 aagttaatat gggggaaaag ttagtagaaa ttgataataa gttacttgaa aatactttat
    16141 atattccttt agttattgta gagaatccta atacggtaaa atatcatctt tcaaacttaa
    16201 atcaggaagg aaatagtggt attaaagtta tcaattttga ttgattatga ttaataaagc
    16261 gattaaattt aaatttatca tattctattt tttctcatat attatttttg cctttgcttc
    16321 aacaaaattc acacctttct tatcaaaatt gggttactca gcgtttgaaa gaggaattat
    16381 attatcgagt tatgcaataa ccaatatatt atttcaatta ttatttggag tattagctga
    16441 taagtatcaa acaatgaaaa aaattgtttt aattagttta tcagtttatg gaattgcatc
    16501 agttattatg ttttctactg aatctgcaac ttttataata tacttattac ttgttgcttt
    16561 atcaggagga ttattaaata cttgctgtag tttatatgat acatggataa taggttgtgg
    16621 agataatatt aataattatt tgtcatttat taagactttt gggtcgattg gatgggctat
    16681 tggaagtatg gtagcatctt acttgattct tagattctca tattttggat tatctgtagc
    16741 gattggttta attgtagtat tacttattat aaatctacta ttattacctg atattgaaaa
    16801 gattaataag aaaataaatg taactaataa agatattatt gaattattaa aagataaaaa
    16861 atatatctta ctagtatgta ttttattctt attatactca atggtagtag caaataactg
    16921 tactgtaata gataaaatga tagctttaaa tgctacaaat tctcagatat ctttaaagtg
    16981 gtctttacag tcattattag agataccaac atatctggct ggttcttatt tattaaaaaa
    17041 atatagcagt ttaaacttat taaagttaag tgctattatg ataacagttc agtttctatt
    17101 atttgcatta acaaatgatt caaatatcat tattatactg tgtgtttttc aagtgttttc
    17161 tactccttta attttaatta cttcaaaaag attaatattt gaagtttctc ctaaaaaact
    17221 tagaagtagt agccaattaa tagctttaag tatatttatg ggaggttcat ctctgattat
    17281 tccaactgtt gctggttttt tgagcattaa tattgggtat aattatacat taatggtatt
    17341 aagtcttttt ggatgcttag cttatggctt gatagtctta ttgaattatc ttcagtaata
    17401 attgttttaa tatatagcag taatatcact tgtttatagt acctgtattt gccacaatca
    17461 gggttgtatt ttggtttttt atagagaaga ataattgtat agattagagg cttcttagga
    17521 gcttcttttc tttgtttata caaagaagta aatcatgctt ttattttaag atatttctac
    17581 tataattata atagtcttcc atatgttaca aaagtgtaat gctaacagtt aaaatgtaat
    17641 atttacttaa ggatttattt atataaaacc tttaaaataa atatatatta aaaaattagc
    17701 aactggaggc tattgttatg aaagaaatat taaaaataaa aaatatatca aaagattatg
    17761 gaataaaagg atttaaaaca aatgtattaa aaaatatatc tcttacagtc aatgaaggtg
    17821 attttatagc aataatgggt ccatctggtg caggaaagac aacattgcta aatttaatgt
    17881 ctacacttga caaacaaact tcaggagaaa taatactaga tggaattaat atatcaaaag
    17941 taaaaaataa tgaactatca aaattaagaa gagaaaaaat agggtttata tttcaagact
    18001 ataatctatt agacaatatg aaactaatgg acaatatagc acttccttta gcattaggaa
    18061 aaaagaaaag caaggagata gaagctaagg tattttctat agctaaaaaa tttggattag
    18121 aaaatcattt agataaatat ccatatcagt tatcaggagg acaaaaacaa agaggcgcag
    18181 cagctaggtc attaattaca aatccaacag tcatatttgc tgatgagcca actggtgctt
    18241 tagactctaa atcagcatat gaacttttag aatcattaga aaaaataaac agagaaaata
    18301 acgctactat tataatgatt acacatgacc ctctaacagc aagttattca aatgaagtat
    18361 atatgataaa tgatggaaat ataaagtgta aattaaacaa aggaaatagt agaaaagaat
    18421 tttatggaaa aattatggat atgctagcct ctatgggagg tgagatgtaa gtgagtatac
    18481 ttggaattgc ttataataat tttaagagta acataagaac ttatctagca ttttttatat
    18541 ctatggtttt ttcagtagtt gtgctaacta attttgaatt attaagatat ggtgatgcta
    18601 taaatatgtt acaagaggaa aataaaaaat ttacactatc agtgttagta gctgtaataa
    18661 tgatactatc agtattttta tttttcttta tatggtatgc aacaaatata ttttttaaga
    18721 ataggtctaa agaaattgga atattgtctt ttatgggatt agatttatat actataggaa
    18781 aaatatattt tgtagaaaat atgcttatag gaataagctc ttgtataaca ggaataatta
    18841 tagggataat aacatctaga ttttttcaag taatgataat aaagttatct ggatttaata
    18901 taaaggtatc taatggactt agcatagaag ctatactgta tgctacttta atatttattt
    18961 ctatatttat aataatggct gtaaaaggtt ttataacaat atgtagaagt agtgtaataa
    19021 atctaatcaa tatatctaaa aaacaagaaa aaataccgaa ggtaggaata ggtatatata
    19081 tattagctat aatatcaatt acagtaatag gatatggcta ctatctttct accaatataa
    19141 gaactggaaa tataactagt gtactacctg ttataggtat aataatagta ggtacttatg
    19201 gcttattcaa atcagttatg cctgttatat ttgatattat aatcaaaaat aaaaaaatat
    19261 tatttaatgg aaataatata attgcaataa acaatataaa ctaccgatta aataaaaact
    19321 ataaaactta tgctataata gctataatta taactacaac aatatcaact cttggtgctt
    19381 cagttgcgat gaagcgtata cataagaatg cccaagaaca aagaaatgtg tacactatta
    19441 gcatagtttc aatggataaa gatgaagtga ataaaaaaca aatagagaat ataataaaag
    19501 atactaataa gataaagtac tcagtaaatc cagaactaac aattttaaag aaaacagata
    19561 taaaagaagc ttatgataca acagatacta taatgaaata tagtgatttt tgtaatactc
    19621 ttaaagtgaa tggcaatgat aaagaattaa aagagtatga tgaaagatta gttagtggaa
    19681 ataatgtaat agagttaaaa agccctcaaa cgatagcatc aatagctaca aaagaagcca
    19741 agataaaact tgatggtcag ttatataata tttcaagggg agaagtaaaa gttccagtac
    19801 tagggacagg acttaatcaa agtattatag tggtaaatga tgatacatat tttaaactaa
    19861 gagataaggg tcaacaagtg tatttttatg gtgcaaaggt atttcaagaa gaaaatagtc
    19921 aagtaatgtt caacaatatt gaaaagaatt taagtttaaa aaatgggtat attcaaaatg
    19981 gatataaagc tcaaggtgag agtcaatgga tgaagtttgc atatgtagtg cttgtgtttt
    20041 tattcttagt ctttgcaatt gtagctggaa gtataatata tatgaaaata tatagtgatg
    20101 catatgaaga taaagagaaa tataagattt tgttaaaaat aggtactaca gaaaaagata
    20161 taaatagagc tatacttaaa gaagtagcta ttttttatac tttgccaatg ttaagtgcaa
    20221 ctatatctag ttattttgca ttgagattag caggagattt attgatgacc gatttatttg
    20281 gtatatatat actaagttta gtgatatgtt tgataatatt cataatatat ggagcagtat
    20341 ctgttaataa gtttaagagt gtagtgtata aaaattagtt gatatattaa taaagagaaa
    20401 ggagattctt gtaataagag tctccttgtt gattaactaa ataatttggg taataaattc
    20461 atataaaata tgttatgata ttaataattc atagcatagt gaaatattag gataatggag
    20521 aatatacaaa tgtatagaat tttattagta gaggacgata ttgatttatc aaaagagatt
    20581 gcattagcac ttgaaaaatg gggatttaaa gtagaattaa ttgatgattt tgaggtagta
    20641 ttagatgaat ttatagataa aaaaccagat gtagtacttt tagatgtaaa tttaccatta
    20701 tataatggat tttactggtg cgaaaaaata agagctattt caaatgtacc attaatattt
    20761 ttatcatcaa gagattctga tatggattta ataatgggta taaataatgg agcagatgat
    20821 tacattacca aaccattttc tatagaaata ttggttacca aaataaatgg tataataaga
    20881 agagtctata attatagtga ttctaatagt attttgtatt gtgaagattt gatgtttgat
    20941 gtggggaaag ggattataaa acataagtat aaggataaaa gtatagagtt gactaaaaac
    21001 gaaataaaaa ttttaacatt attattaaaa aataagaata gggttgtatc aagggaaagt
    21061 cttatgatga ctctatggga taatgatgag tttgtgacag acaatgcact tactgtaaat
    21121 atgaatagat tgagaagtaa agttaaagaa ctaggttttg atgattttat aaaaactaag
    21181 aaggggatag gatatataat acaatgctag taaaatattt aaaaggtagg tctaagtata
    21241 ttgctctacc tttagttgta gttagtataa taaatatcta cttatttgct atagatattt
    21301 ttaaaaataa atattcagag ttaatatatt tagatttttt agtactgttt ataataatgg
    21361 tattttttgt tatagattat ataaatttta gaaatagtta cacaaattta tataattgta
    21421 tagaaaatag tggtgaaata gacagttatt tagttaatgg acaatctttt gaagaaaatc
    21481 taataaaaga tattatagaa aacttaaaaa taaaaaataa tagtgacata gagacttata
    21541 aacaatcatt aaaagaattg gatgagtaca tagctaaatg ggttcatgaa ataaaaattc
    21601 caatatcttc tttaagtata attacagata ggttaagtag catagaagat agtttggata
    21661 ttaagaatca ggttgcaaag ataaattttt tagtaaactc tattttgtat agtagtagaa
    21721 gcaacactat gtttgaagat gtatttataa ataaatttaa tttagaaaaa ttggtaaaaa
    21781 tgtctataaa aaataattca tttttgctta taaaaaataa tgtagaagtt agtttgaatg
    21841 aattagaaaa tgatgtttac acagattcta aatgcatgtc atatgtttta gaccaaatta
    21901 ttaataatgc tataaagtat tcaaaggaag ttggtaagat tgaatttaat tcaaaaaagt
    21961 tagaaaatgg agtggtatta tctataaaag attttggcat aggtataaat gaggaagata
    22021 tatctagggt ttttgacaag ggatttacgg ggaaaaatgg aagaaatcaa ttgtataagt
    22081 ctactggaat ggggatgtat tttgtaaaga aaatgataga tagtttaggg catgagattg
    22141 aggtatgttc gaaaattggt tcatatacaa tttttaatat ctacttttat gatatatcgg
    22201 attatttaag cctagatagt taggttatta gactataaaa tctatcatca tatacaaatc
    22261 gacatataca ataagaataa atttagttct tgaaaatagt gaagatttaa ttagtgcctt
    22321 aaatgatgct ctatctatat tataaaataa taattattat atatgaaaaa catatcagat
    22381 aggtacctgg tatgtttttt atttgtagta actattttaa tatggttttt ggagagagaa
    22441 tatattttgt ttgtgaaaaa aaattacatg gtattacatt gacactgaaa ataaattaat
    22501 gtaagattta gctaaggtaa aaaacgtgta taagattaac tcaattaatt tcataagagt
    22561 aaattatcgt aattaaagat gaaataagat taattctaaa attattaaac ttaatacctt
    22621 gatttgggga ggaataacta taaacttaaa aggatgataa ctatgtatag tgtaggttta
    22681 atgtcaggaa cttccttaga tggagttgat gcagtactag ctgaaattag tgggaatggt
    22741 agaaatacta aagttaagca aatagaattt ataactctag aaattcctaa agatataaaa
    22801 gacgaaataa gaaagtgctg tatagaagag gaatcgagtg ttgatttaat ctgtagctta
    22861 aattttaagc ttggatattt atttagtaaa gctgtaaaat cagtgtgtca taaagcaaac
    22921 tttcatatag caaatttgga ttttatcgca tctcatggtc aaacaatctt tcatatacca
    22981 agaagttata ataattttgt atcttcaacg cttcaaatag gtgaaccagc agttatagca
    23041 tatgaaacaa atacaaaggt aatatctaat tttagagtaa tggatatagc agcaggtgga
    23101 gaaggtgctc cattagtacc atatagtgaa tttttattat atagtgataa caataaaaat
    23161 ttagcacttc aaaatattgg tggaatagga aatattacta taatacctaa atcatgtaac
    23221 atagatgatg tatttgcatt tgatacagga ccaggaaata tgataataga tggagtatgt
    23281 caaagattat ttaatagaaa gtatgataaa aatggatact ttgcatcaaa aggtaaaatt
    23341 aatgaagaaa tgttaaaaga tttgatgagt cataagtatc ttagtcaagc accacctaaa
    23401 agcactggaa gagaggtatt tggacaagtc tatttggata acatgcttaa caaatataaa
    23461 catgtagata aatatgattt gatagcaaca gtaactatgt ttacggcaaa agctatatat
    23521 tataattata aaaacttcat actttcaaag gttaatgtag atactttatt aattggaggt
    23581 ggaggagctc ataatttaac tttaatagga tatataaaag agttattatt agaggttgaa
    23641 gtattaacac aagatgaata tgggtattct tctgatgcaa aggaggcttt ggcatttgta
    23701 atcttgggca atgaaacatt aaataattct ttttctaatg taatatctgc aacaggggct
    23761 aaaaataaag ttattttagg gaatataaca cctaaacctt ttggaggtaa atgacatgtc
    23821 taataataaa aaaaagaaaa gaaagaaaaa taataattat aaaaatgcaa aaagtacaaa
    23881 aagtaataaa aatactaaga aacaaatgga gaaaaatcaa agaacagatt ataatattcc
    23941 aactataagt tttaaaagtg gattaatact aatagtttca tctcttgtaa gtactgtagt
    24001 ttttcctttg ttattatcta tatttggaat agataataga tttggagttg ttataggaaa
    24061 ttcattaatt acatcttttg cagttgcata tacaagatac tttgttgaaa ctaagagagg
    24121 tttttctttg ggatttttta gactgtatat tttatttgct ttatcatttg caataatagg
    24181 ttacttctgg atatataaag gaatacatat ttaaaaaagt attgagacta ttttatgtta
    24241 ataataagat agtctttttt ctttttaaga aaagatatga gtattttaag tagtgtgtaa
    24301 ttttagacaa aagaatgatt agataaataa agcaattaaa acaagatata aattcatgaa
    24361 ataaaattac aaataaaaat gtaaaatatg aaaaaatata tacaaacgta ttcctgtaat
    24421 caaaaataca tgctataatg attgaaaaga aataaaaaaa cagggggaat caatatgaga
    24481 gtattatttg tttgttcatc agggatgtca agtgctatag cagttaatgc attacaaaaa
    24541 gaaggagcaa aaaatggaat agatatagat gttttggctg ttggtactca agagtttgag
    24601 gatgaagtta aaaatggatg ggatattgct atggtagctc cacaagtcag acatagattt
    24661 gactatttaa aagcttttgc tgatgaggtt agtgtaccat gtgcattaat acaagcacaa
    24721 gcttatagtc cattaggagg accaaagtta ttaaaacaag ttcaagagct gttgagtaaa
    24781 tagtagttgc agtgttgtta ggttagaaga aagttaattt tataaaaatt ttaatgataa
    24841 taaaaataaa tattaattaa ggagagaaaa gatgctagat aaatttacag aatttttaga
    24901 cactaaatta tcaacttcta tggccagatt ggcagaacaa cgtcacttaa gagctgtaag
    24961 agatggaatc gtagcttcat taccacttat aatagttggg tcatttttct tgatattagc
    25021 atttcctcca ttaccagatt catggggggt aactcagtat ctaaaggaaa atgcagttac
    25081 aatattacta ccatatagaa tgacattttt tattatatca gtatattcta catttggcat
    25141 aggttatagt ttggcaaagt catataagtt agatggattg acaggaggtt tattagctac
    25201 agctacattc cttttgacaa tagttcctac tgcaatagag ggtcttaccc ctgatttatt
    25261 aaagatagtt gaaaagagtc cagaactttt agcttggtat aattcaattc ctcaaggatg
    25321 gcaaatgcca atggctaatc ttggtggagg ttcaatgttt gttgctattg taacatctat
    25381 aataggtgtt gagatttata gatttactga caaatctaaa ttcaagataa ccatgccaga
    25441 agcagttcca gcatcagtca caaggtcttt tgaagctctg acaccagccg cattaataat
    25501 aattttaatg gctattgtta cttatttcct agggtttgat tggcatggct tcatagctaa
    25561 attagtaact ccattagttt cagccactga cacattacca tcagtaatta tactaatatt
    25621 tttaataacg tttttttggt catttggaat acatggtgta tcaataatag gttcattggc
    25681 aagaccttta tggatacaat tattagatgc taatacagct tcatatgctg cgggtcaagc
    25741 agttccaggt attgctgctg aacccttttt tcaatggttt atatggattg gaggttctgg
    25801 agctacaata ggcttagctt tacttcttgc atttagagca aaatcagctt atggaaagtc
    25861 tttaggaaaa gcaactgtaa taccagcaat attcaatata aatgaaccaa taatatttgg
    25921 agcaccaata gtattaaacc caatattaat aataccattt gttgtggcac caatagtaat
    25981 ggcaacagtt tcatggtttg ctatgagtgc taatttagta tccagagtag tagttactgc
    26041 tccttggaca ttaccaggac cagtaggggc atatttagcc actggtggag attggagagc
    26101 ttcagttctt aacttattat gtatagctat atcaataata atttactatc catttgtcaa
    26161 gatgtacgat aagaggttgc ttcatgaaga aaaagttgaa gagcaagaag cttattaatt
    26221 agaaactata ttggaaatta aaaactatat tagattagta taaatttcaa agtataacag
    26281 ttatgaaata agtaacgaag atattttgtt gattgtttat aaaaaaagaa aaggtaaact
    26341 tttctttttt tataaatata tatgaaactt tttttcatca aatttttaaa aaagtttcgt
    26401 gtaattcgaa ataaatttaa acacatatag gatataagga gatgacacag atgggaagat
    26461 taggaatatc aatttatcca gaaaaaacaa catctaagga aatatatgat tatatagata
    26521 aagctgctga aaatggattt acacgtatat tttcatgttt attatcagtt aaagatacta
    26581 aagaaaatat aattaaaaaa tttaaaagta taaatgaata cgctaagttt aaaggttttg
    26641 aggtaatact tgatgtaaat cccagagtat ttggtgaatt agaaataagc tacaaagatt
    26701 tgaatttttt taaaaagacg aaggcagacg gaataaggct tgatatggga tttacaggtt
    26761 ctgaagaggc attaatgact tttaatcctc aaaatcttaa aatagaaatc aatatgagta
    26821 ataatactaa ttatatagat acaataatgg actaccaacc aaataaagaa aatttgatag
    26881 gatgtcataa cttttatcca catagatata gtggattaaa tttagaacac tttataaaat
    26941 gtacagaaag atttaataag tatggattaa gaacagcagt ttttgtaaca agtcaaaatg
    27001 aaactacatt tggaccatgg cctgtaactg atgggcttcc aacaattgag atgcacagaa
    27061 atctacctat agatattcaa gtaaagcata taatagctat gaataatata aatgatataa
    27121 taatatcaaa ttgttatcca acatatgagg agctaaaggt attaggttct atgagaaaag
    27181 atatggttac atttgatgta gcaatagagg aaaatatacg agaagttgag aagaagatac
    27241 tctttgaaga aatacatttt aatagagggg acatatctga tagtctgata aggtctactc
    27301 aatctcgtgt taaatataaa ggatacaaat ttgaactatt taatactcct aaaataataa
    27361 aaagaggaga tatagtaatt gaaagtagtg aatatggtca ttatgcaggg gagttacaaa
    27421 tagccttgac tgatatgaaa aacagtggta aatcgaatgt agtaggatat ataagaaaag
    27481 aagaattatt tatattagat tatattaaac catggcaaaa gtttaatttt aaaccctcaa
    27541 caaaataatt tattatgaaa atattaattt attataaaaa tataattatg aaagttggaa
    27601 ttaaaatgtg tttaaaaatt taatattaag aaaatgagga aatgatatgt taataaaaac
    27661 tttagaaaat ttagttacag aaggtagaaa taagaataca ttgcaaatag ataaagaaga
    27721 tacattaggc ataatagaat taataaataa cgaagataaa actgtagcct atgctgttga
    27781 agaacaaaag gagagtatag caaaagctgt taatataata gtagatagaa tgaaacaggg
    27841 aggtagatta ttctatatag gagcaggtac cagtgggagg ataggaatac tagatgccac
    27901 agaatgtcct ccaacttacg gagtggattt tgagcttgtt caagctataa tagctggtgg
    27961 aaatcaagca atatttaaag caatagaagg tgctgaagat gataaagaac ttggaaagca
    28021 agatataata gatagaggag ttacatctaa ggatgttata tgtggtatag ctgcatcagg
    28081 aaggactcct tatgttatag gtgcaatgga gtatgctaag gaattggggt gtgcagtact
    28141 atcaataact atgaatccga atagtgaaat gagtaaaaaa gcggatttac caataaacat
    28201 tatagtaggg gcagaagtaa taatggggtc aactagaatg aaaagtggta cagctcaaaa
    28261 aatggtatgt aatatgttaa caacagcttc tatggttaag atgggaaaag tatactcaaa
    28321 cttgatggta gatgttaaga catcaaatga aaagttggtt gaaagagcaa aaagaataat
    28381 aatgatagct acaaatgtaa aatatgacgt tgcagaaaaa ttcttagaag aagcagataa
    28441 tagcgttaaa ttggctatat ttatgataaa aagtggttta gataaggata gtgcaaagag
    28501 tatcctagat agacaagaag gttatataag tgaagcttta aaatcaatag aaaaattgta
    28561 aaataaataa tagtactgat aacctttcaa tattaaaaaa acagcattgg aaggttattt
    28621 ttagatatta agtattgaaa gaattattaa agatattgta ggaggatagt atgtattttt
    28681 taggtgtaga tggtggagga acgaaaacaa catttacatt agtagatgaa gaattaaata
    28741 tagtaggaac cataaccaaa ggaacttgtc attataatca aataggattt gataatctaa
    28801 cgaaattatt aataactggg ttggaagaag tttgtaaaga tgctaagata aatgtagaag
    28861 aaataacgta tgcatttgtt ggacttgctg gatatggaaa aataaaagaa gtattgtatg
    28921 cattagaagt agcaacaaaa aatgcatata gtcatataaa ttatactcta ggtaatgatg
    28981 tagaaatagc attagcaggt tctttaaatg gggaaaaagg tataaatata attgcaggta
    29041 ctggttcaat agcacaggct ttagataaag atgggaatct tcacagatgt ggaggatggg
    29101 gctatgtatt aggagatgaa ggttcagcgt attatatagg aatggctacc ttaaaaatgt
    29161 tcacaatgca gtctgatgga aggtgttcaa aaacaaaatt atatgattta ataaaaagac
    29221 atcttaatat agagaatgat tatgacataa taaaatatgt aaatgatgaa atacaaggag
    29281 atagaataga aatagcaaag tttgctacga tatgtttaaa agcagttatt gaaggtgata
    29341 atactgctag taaaatattt gatgatgcag cttatgaatt aagtaggctt ataataggat
    29401 tagagcatca ttttgaacaa ggtacaaaga taaaggtatc ttattcaggt ggagtgttta
    29461 agtcaggaga tttaatatta gagccactta aaaaatattt gaataaaacg agatttggta
    29521 tagtgagacc agtgttgact ccagatttag gagcatgttt acttgctaag aagaaatatt
    29581 atagtaaata ggtagaggtg atagagtatg agttgcattt taaaattaaa acagatatat
    29641 gaagatttaa cggaagtaga taaaaaaata gctgattaca tacttaataa tacagaggct
    29701 ataagtaagc tttctgtatc agaattggcg tcaaactcaa aaacaagtac agcaagtata
    29761 gttaggtttt ctagaaaaat gggatattcg ggttttgggg atttaaaaat tgagatagct
    29821 aaagatttga tgggcaaaga aaatgaatac acatatgtag atgaaaacta tgattgtaat
    29881 atagatagtg ttataaacaa aattacaaac aaaaacatag agactatcaa ccaaacaagg
    29941 ttgttgaatg aaaaagatat aataaaagaa gcagttgaac agataataaa ggctaaaaat
    30001 gtatatatat ttggagtagg tggttcggct ttagttgcac tagatttaca gatgaaactt
    30061 ttaaggataa ataaacaggc atttacctct ttagatagtc atacacaact tatggtatct
    30121 tcaaatgtag ataaagaaga tatagctata gcaatatctt actctggaga atcaaaagaa
    30181 gtaataaagt ctatagaaaa tgcaaaatta aaaggatgta aggtcatttg tataactaaa
    30241 tatagtgaca atcatcttag taagattagt gatttaaagc tagtagtacc aaatatagaa
    30301 aaaaggctta gagaaggagc tatttcttca agaatagcaa tgcttacgtt gatagatata
    30361 atatatattt ctataattca agaaaactta aatgaggcag aacagaaact acaagaaaca
    30421 aaaaggatac ttgattattt aaatattaat aatgcttaaa actaggttat atatcaacta
    30481 aacaaataag ttagactata agcgtaagtc aataagaaat agataaatat ctaaaatatt
    30541 gacttactta ataataatat atagcatttt tataatacaa atgagttgtc ttaaaattga
    30601 ttttttttaa tcaaatttta agttcaactc cttttttata tttttataga tatatctata
    30661 ttttcccaga aaacgtattg aatatacaaa ttataagaag tataatgttg ttgggagaga
    30721 atgtatgaaa tgtatacaaa gttgatttta actgtattat tgataattct ctttactctt
    30781 accattatta atgattgaag acaatggtat ttattatagg tcaaagattc cgaatgtatt
    30841 aatcatcatc attttctcaa actaacagca aacaccctga tttgcgagtt gtattgtata
    30901 caaattatct agaaaatatc tattagattt tccatgaaat ttaaatattt cacgaatgta
    30961 tgaatggaga aatatacatg ttgacaatcc acctttttaa atcctaaatt aattttatta
    31021 atggtagaga ataggaagta acaatgtttt ataaaggaga atattaagta tgaagaaaat
    31081 taggaagtta gctaccaagt tattgattac aacaatagtt ttaatctcag gtatgagtat
    31141 gacagtttat ggtatgacag ccaaagaggt aacagcaaaa acaccaaaaa gctatgtcac
    31201 tggaaccaat agcgtatatg gtccaaagct gtcacaagcc caactaaact cagtagcaca
    31261 agcaacagca gattttatga acaagaaaat cacaaagaat atgaccacag atgctaagat
    31321 tttagttgcc tacaatcaca ttaaaaataa cacaacatat gttgactgga atgcagtaga
    31381 gggagcaaat actgcctata cattggttac taaaaaaggt gcatgctcag gaatggctag
    31441 gagcatgaaa gctttgtgtg atgctatggg aattgaatct tattatgtac acagcacatc
    31501 aaatgaccac cagtggaatc ttatccgttt tggtgatggg cgtttgtatc atgtagatat
    31561 tgatgctaat aaatcagcag gaaaggatat catatacaag agtcttagcc acccattacc
    31621 atttgataag acagcatatc caaaagttgg cgcaatagta cagacggtaa aaccagaaca
    31681 gacacagaaa ccagcaccac ctgggaagca cactatgtta ccacatcaag aaggggcaaa
    31741 ggctgttgga aattttggaa aaaaaggaga agatataaat gaaaaacatg taatgtatga
    31801 aggtgttcga tgtagtgtag ttaattcatc agtaaataca attacattta caggtagtga
    31861 atcaaattta gacccatcaa atcttacgat tgctgtaata ggatttatga acacaccata
    31921 tgggatgttt gcattagcac cagagggtaa acctgttgaa tatcctttta aatatgataa
    31981 gccattttct ttagatttta gtggtaaaaa tcgttctggt gcagaattag cgaagtactg
    32041 taaaaaaaat gatgtgacta ttaaaataga aatcactgat gaaaaagcta atattggact
    32101 tggtcaaata atgtatgttc gatatgaata atgatttaat catgacagta gaataagtta
    32161 taaacattgc gccctcttac taggggcgca tttaatatta tatctgatat tttgtagtca
    32221 ggtcaactgc tagatttctc ccctttttat ttataaaaat tattatctct aagcatctga
    32281 gcatgtttac gattaactaa aaactgtttt aactcagtca agcataggaa taaaatagct
    32341 ctattttcaa actctgttct gtcaacaggc agaggctggt ttttcatact aagcattagt
    32401 ctctttaatt cttcaagaag aagattgcct gtttcagctt caaattcagt ataacctatc
    32461 ttatgtataa aagcagatag aatatgagct tgtggtggtg tagaatttag ttgagataca
    32521 taactataca aattttctaa gatatcccgt tggcttttta taatatccat atgttctaag
    32581 aagaatctag tatcacttaa taaagtatta ttaatattat cgtaagtttc agaaatactg
    32641 ctgtcaataa gtgaatttaa tgtatataaa tctctactat atccattttc ttttttagga
    32701 tttacaataa tatctgcaat gtctataagt acaatactca cttcttcctg taactttttt
    32761 tgaacttcat ggattttatg tatatttgtt ggaatataca tatttattaa gacacctatt
    32821 ccagttccta ctgcaaatat tccagcttca tttaaaatcc atttgataga agtttctcct
    32881 tgaaggtagt aatgggatgc tatgactaca cacatagcaa taacatcttg aatatttaac
    32941 aaaaaacaaa gtggtataat tataaatata aatattgaaa atgaagtcaa attatatcct
    33001 aaaatattaa aacataaata tgaaaaaata gtacataaaa gaaaaccata aatctttcca
    33061 atggagcctt taagagtttc tcttttagtg tcctttactg taagtagtac tataactcca
    33121 gcaaccattg aatattctaa ttttaatagc caagcaataa atatagcaag agaacatcca
    33181 agagataatt ttattacttt aagtgaatta atattattca aatgcaaacc tcctgtaaat
    33241 caatataata aaattctaac ataaaactaa tattgcttga aaggtaagta actatactta
    33301 attattttat tgccctattt gtttagatac aatcaatata attttatttt atgaaaattt
    33361 tttgtaaaat tttaaattta gagactaaca tgagaccaca aagaggaagt ttattatagg
    33421 aagaaaactt aaaattgatt ttaagcagca aatgaaaata atcaaatatg tatatactat
    33481 tattaaaaaa taaaattata tagttaatat aattttataa ttggtttagt atagagattt
    33541 ttaactttta tgttatacta tttataaaag taggtataaa gattaaaata cataatcaaa
    33601 tatgtataag aggttttaaa tagtattgtt aatgaacttg attattgttt ataattacaa
    33661 attggaggag ataaacatga aaattactga aggatatatg ccttttaaag gttttaaaac
    33721 gtattaccgt atagtaggag aaaatacgga agggaagaag ccattagtat tacttcatgg
    33781 aggtccaggt tctacacaca actattttga ggtattggat aagatagctg aaagtggtag
    33841 acaagtaata atgtatgacc aaattggttg tggaaattca tttgtagaag ggcatcctga
    33901 gctatttaat gcagatactt ggatagagga gcttattgaa ctaagaaaac atctaggact
    33961 tgatgaaatt catctgttag gtcaatcttg gggaggtatg caggctattt ggtatgctat
    34021 tgaatataaa ccaaaaggta ttaagtctta catcctttca tctactcttt cttctgcaaa
    34081 actttgggag aaagaacaaa aaagaagaat atcatatatg agtgaggctg accaaaaagc
    34141 attacttgat gcagtaaata ctggagatta ctctagcaaa gaatataatg atgcattaga
    34201 aagatttatg gaaatgtatt gtgcaggtga agtgacagaa gattctccag agtgcctgag
    34261 aagacctaaa aaatcaggtt ctgaagcata tattgtagga tggggacaaa atgaattttc
    34321 tcctacagga actctttctg gatatgaatt tacagataga ttacatgaaa taaaagagcc
    34381 ttgtctagta actagtggag caatagattt atgttcacca tatattgcaa agactatgta
    34441 tgatagaata cctaatagta agtgggaatt atttgagtat tcaagacata tgccattcgt
    34501 tgaagaaaat gagaaatata taaaagtctt aactgagtgg ttgaatgaga acgattaaaa
    34561 actatataaa tttatataaa aaaatgagtg tctcaaaatg aactttttag ttcattaggc
    34621 actctttttg catgaaatca aatatatttt cattatttga acgataaaat gaggcttata
    34681 tctattttgg gaaagtaatt ttttattact agcgttagat tctaatctaa ttgtatactt
    34741 attgaaataa tagaaatata ttggtgttta taacttaatt ttattgtttt ttagaaattt
    34801 ttttatatat tggtataatt attctgttta aatttgatta aaaagtatat taattataat
    34861 aagaaacaat tatataaata ggaatagatt attataatta atatagatat aaaaataatt
    34921 atagaaagaa aggcacatta tggaaaaatt gagagttatt gaaatgataa aaaataatcc
    34981 caatattata gctacaatag ataatccaac tgatgaaatg aaattgttag ctattaaaga
    35041 aaatgggctt gtactagaat atataaataa tccaaccaga gagatgcaag aactagcaat
    35101 agataataat attagagcaa ttaaatttat agataatcca acagaagata tgatgataaa
    35161 agcagtaaat gagggatgga gtatactgga ttatatcaaa aacccaactg ataaagttat
    35221 tgaaatggct ataaatcaag caggatgggc aattaaatat gctaaaaatc caagtgaaga
    35281 attacaactg ttagcagtaa gaaaaaatta tgattcgata aagtttataa aacaacctta
    35341 tgaaagtgta caagaggagg cagtgagaat aagttatgat gccttgagat atatagattc
    35401 acctagttat aatgcagaac ttatagctat aaaaaataat gaagcagcta ttagctttat
    35461 aactgattta gataaaaata aaatgttgga atttttaaaa gtgaatattt tagtgattaa
    35521 gtacattctg aaaaaagtat ctaaagatga attagagagt gtattaaaag aagtattatc
    35581 caaagaagat gttgaggaaa agtatattag agattttttg aattgtagtg ttatagatag
    35641 aaatagtgaa agtataccaa tagataaaat tatgtttatt tataaatatg gaagtaagac
    35701 agcaaaaaaa atagctgtag atgagaaatt aaagattatg taggagagag aaaaatgaat
    35761 tttatagaca cattaaaaag cgttgactta atattatcca ctggagaagt acctttaata
    35821 gtaggtgaaa gtggaatagg aaaaacagct ttagcaaata agcttgccaa agaaaatgat
    35881 tggagtttaa ttgttattga tggaaactta cttaaagagg gtgaaatagg tggtcttcca
    35941 actatagaat cttatgtagg agttaattct aatggatata agactgaaaa gaaaacaaca
    36001 gtgtatgctg ttcataacaa attaagagaa attgatgaag agatatctag aagtaagaca
    36061 gttcttttat ttatagatga gataaatcgt tgtgagcata cagtacagca agaacttatg
    36121 aatttaattt taaatagaga aattaatgga tataaactgc atgatgatgt aaagatttta
    36181 gcagcaatga atccatcaag taaatatggt tcagattttg attatcaagt ggtagatatg
    36241 gatgcagccc aagaaaatag gtttgtttgg ttaaatatgg agagtgacca tacacaatgg
    36301 ataaaatggg caatagatga gggtattgaa agaaaagtta tcgagtttat ttccactttt
    36361 ccagaatatt tgcataaaat aaatgaagat gatgtaagag ctactcctag aagttatgag
    36421 agagtatcta aaatttataa agtttataaa gaaaaaaata attctattcc tagagcagta
    36481 tttttaaatg ttgtaaaggg taatgtaggt aaagttattg cagaagaatt tataagtttt
    36541 attgaatcaa actctgaacc attaatatcc tatgaagatg tttttttagg agaatctatt
    36601 gatgagtcca ttgtagaaag agtaaaaaat gagagtcata caagacttta tctatcagct
    36661 atgaacattc taaaagactt agaattaaat attaagaatg ataaatatga atcaaaccac
    36721 tatattaata gatttattga atttttaaaa atgtatcctg tagatttaat gataggtatt
    36781 atgaaggata ttagaaacag ttatattgaa gtatataaaa aagctataga aaatgaagag
    36841 tttgtaaaat catattttga atcttatagt ttaataaggg gataatgtat ggaaaattat
    36901 tttgataaac aagcaaaaga gctttataat aaagcaagtg aaattataaa tactcattct
    36961 atgctaaagg caaatcgtgc taatgagaaa tttgatatag atataccaca agattttaag
    37021 tatgagtttt ttagtcttgt agataaagtg aatttaagtc ttatggaaga agaagataat
    37081 ttttatggat attttttgtt tcagatgtca agagaaataa gatttgatat aagtagtcct
    37141 acaggtgtga attttaaggg agctaaatat attatatatt ttaatccaat aatcttttta
    37201 acgcttgaca taaagcaaat ggagactaca attaaacatg aaattcatca tatattatct
    37261 atgcatttga tgagagcaaa ggagttaaaa ggaaagtata gtacattagc aattaatatg
    37321 gcaatggata tagtagtaaa taaatttcta aacaatttac caccatatgc aactacctta
    37381 gagtgggtaa atttaaagta ttccttaaaa cttgaaccat atgaaccttt tgaatattat
    37441 gtagaaaaga ttcagactga attagatttg atggaagtgg atgaagatgg tgaggaagat
    37501 gatagtgata aatatggaga tatagaaatt gattatagcc ccgaaagaac tcatgatata
    37561 tgggatgaat ccaatgagat agatgaaaat acccttagag aatttactga aaaatttatt
    37621 aacagttctc aaaaaggtaa agttcctgct tatttagaaa gtatgatatc atcacttaaa
    37681 aatagtaagg gtgaattgcc atggaattta tatcttaata gaataatggg aacagttgaa
    37741 aataataaaa agaagactat aacaagaaga aatagaagac aaccaaatcg tttagatttg
    37801 aggggagaac ttagaagtca taaggcagaa atagcagttg cacttgatgt aagtggaagt
    37861 attagtgatg aagagtttaa acaagctatt aaagaagttc ttagtatagt gaaaaactat
    37921 aatcatgaaa ttacaattat agaatgtgat aatgaaatta gacgtgtata taaagtgaga
    37981 actgaaaaag atataaaaga tagaattagg ataggtggag gtactagatt ttctccagtc
    38041 tttgagtatg ctaataataa aaagattaat ttgttagtct actttactga tggtaaaggt
    38101 gaaaataaac tacaagtaat acctagaggg tataaaattt tatgggttat ttctggaaga
    38161 ggagataaaa tttcattaaa agaaccttat ggagcagtta aaaaacttac taaggttaaa
    38221 acaaatgatg aaatattaga aataaatgat gtaagaaatg atgggtactc aatgaatagc
    38281 caagcaccaa ttttataggt attcacagga gataagcttt attaatttat tttgatatgc
    38341 ttgttataaa aatctaaaaa atacattaat caatggttaa tgtatttttt aattttaata
    38401 atataagttt tacttacatt taagtcttaa aatgtgttaa aatataatat atagcagata
    38461 gttttggcaa ggtggattta gttgtcaaaa ctattttttt tattaaggat ggtgatgttt
    38521 ttgtttaaga ttgatgatta tataatgtat ggtatgacag gagtctgcaa agtcatggat
    38581 ataacaaatg aacgatttac aaatggaata aaaaaagagt attatgtatt gagtcctatt
    38641 tattctaata atacagtaat aaaaattcct gtagataatg aaaaagtacc aatgagaaag
    38701 ttgctttcta aaattaatgt gctttcatta ataaatgata ttcctaatat ggatacatca
    38761 tggatagata atgaaaaatt aagaagtgag caatttaaaa aaatactcag aggtggtaaa
    38821 tgtgaagagt taataaaatt agtaagaagt atagataata acagagagta tgtaaaatct
    38881 attggtaaaa aaacacatca agcagatgat aatataatga aagaagctga aaggttatta
    38941 aatgaagaat ttgcaactat tttagatatt tctcctaatg aagttagttc atatatttca
    39001 agtcatatac ctcaataaag tgttttagta tcctttattt aatctatttt aagtattatt
    39061 tatgtatgct ggttttggta aaataaaata tataattaaa ttatataaaa aataataatg
    39121 attgtattga agaattacag aatagtgcgg agagcccact cctttaggtg tgagatggat
    39181 attacttaaa gatttaaatt gttattgtag atgtttttgt actttttaat ataaagcaag
    39241 attaataagt gtattgtaag ctttaagtga aattgatact ataatgagtt acttagattt
    39301 taaaatatat aatattataa aaaatccttt taggttttga atgaataaga tggatactat
    39361 gttgaataga atccatctta tttaagtttt atttatgttg tttatttgta agcattattt
    39421 ttgtacatga aaacacagtg taaaaatatg cttagaatta tgaagtttta ttaaaaacat
    39481 attcgacaaa atgagactaa aaatattctt aaacttataa ctaagataaa tattgtatat
    39541 ttatagtgta gcctgaaaaa ttgtggatgt acgaaattta tttagtattt tattgaaaca
    39601 aagtagaaga agtactatag tttatttttg aatagattgt tattttttag tgaaaaagtt
    39661 attaggttaa tacataaata taatagttta taaatagttt aagcatgtta aaaaagtagt
    39721 tattagagat atatagttac attgcttata agatacttaa aatgatagca ttcacttata
    39781 tttagaaaat
//
LOCUS       DAMJYC010000029        38468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJYC010000029 DAMJYC010000000
VERSION     DAMJYC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.030"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(180..1520)
                     /locus_tag="PC472_001665"
     CDS             complement(180..1520)
                     /locus_tag="PC472_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2726992.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
     gene            complement(2190..3281)
                     /locus_tag="PC472_001666"
     CDS             complement(2190..3281)
                     /locus_tag="PC472_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2726993.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(4049..5968)
                     /gene="thrS"
                     /locus_tag="PC472_001667"
     CDS             complement(4049..5968)
                     /gene="thrS"
                     /locus_tag="PC472_001667"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2726994.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     misc_binding    complement(6032..6270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6882..7484)
                     /locus_tag="PC472_001668"
     CDS             complement(6882..7484)
                     /locus_tag="PC472_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2726995.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(7664..8482)
                     /locus_tag="PC472_001669"
     CDS             complement(7664..8482)
                     /locus_tag="PC472_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2726996.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(8485..9246)
                     /locus_tag="PC472_001670"
     CDS             complement(8485..9246)
                     /locus_tag="PC472_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2726997.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(9736..10848)
                     /locus_tag="PC472_001671"
     CDS             complement(9736..10848)
                     /locus_tag="PC472_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2726998.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(10974..11978)
                     /locus_tag="PC472_001672"
     CDS             complement(10974..11978)
                     /locus_tag="PC472_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2726999.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFMGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(12266..13114)
                     /locus_tag="PC472_001673"
     CDS             complement(12266..13114)
                     /locus_tag="PC472_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2727000.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(13150..13620)
                     /gene="trmL"
                     /locus_tag="PC472_001674"
     CDS             complement(13150..13620)
                     /gene="trmL"
                     /locus_tag="PC472_001674"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2727001.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(13814..14983)
                     /gene="nth"
                     /locus_tag="PC472_001675"
     CDS             complement(13814..14983)
                     /gene="nth"
                     /locus_tag="PC472_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2727002.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            complement(15181..17418)
                     /locus_tag="PC472_001676"
     CDS             complement(15181..17418)
                     /locus_tag="PC472_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2727003.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(17524..18438)
                     /gene="queG"
                     /locus_tag="PC472_001677"
     CDS             complement(17524..18438)
                     /gene="queG"
                     /locus_tag="PC472_001677"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2727004.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            19460..19864
                     /locus_tag="PC472_001678"
     CDS             19460..19864
                     /locus_tag="PC472_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2727005.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(20121..21116)
                     /locus_tag="PC472_001679"
     CDS             complement(20121..21116)
                     /locus_tag="PC472_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2727006.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(21400..22236)
                     /locus_tag="PC472_001680"
     CDS             complement(21400..22236)
                     /locus_tag="PC472_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2727007.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(22724..23287)
                     /locus_tag="PC472_001681"
     CDS             complement(22724..23287)
                     /locus_tag="PC472_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727008.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(23385..24314)
                     /gene="glsA"
                     /locus_tag="PC472_001682"
     CDS             complement(23385..24314)
                     /gene="glsA"
                     /locus_tag="PC472_001682"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2727009.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(25265..26932)
                     /locus_tag="PC472_001683"
     CDS             complement(25265..26932)
                     /locus_tag="PC472_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2727010.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(27006..27761)
                     /locus_tag="PC472_001684"
     CDS             complement(27006..27761)
                     /locus_tag="PC472_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2727011.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(27771..28307)
                     /gene="lepB"
                     /locus_tag="PC472_001685"
     CDS             complement(27771..28307)
                     /gene="lepB"
                     /locus_tag="PC472_001685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2727012.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(28473..29009)
                     /gene="lepB"
                     /locus_tag="PC472_001686"
     CDS             complement(28473..29009)
                     /gene="lepB"
                     /locus_tag="PC472_001686"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2727013.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(29109..30419)
                     /locus_tag="PC472_001687"
     CDS             complement(29109..30419)
                     /locus_tag="PC472_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2727014.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(30498..31214)
                     /locus_tag="PC472_001688"
     CDS             complement(30498..31214)
                     /locus_tag="PC472_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2727015.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            31459..32730
                     /gene="sleC"
                     /locus_tag="PC472_001689"
     CDS             31459..32730
                     /gene="sleC"
                     /locus_tag="PC472_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2727016.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(33156..33812)
                     /locus_tag="PC472_001690"
     CDS             complement(33156..33812)
                     /locus_tag="PC472_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727017.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(34924..35334)
                     /locus_tag="PC472_001691"
     CDS             complement(34924..35334)
                     /locus_tag="PC472_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727018.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(35550..36932)
                     /locus_tag="PC472_001692"
     CDS             complement(35550..36932)
                     /locus_tag="PC472_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2727019.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(36943..37302)
                     /locus_tag="PC472_001693"
     CDS             complement(36943..37302)
                     /locus_tag="PC472_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2727020.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(37507..38286)
                     /locus_tag="PC472_001694"
     CDS             complement(37507..38286)
                     /locus_tag="PC472_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2727021.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        12651 a         6946 c         3564 g        15307 t
ORIGIN      
        1 atacatatta aattcttata tattaaactt ttatgcatca cttttataca ttagttctta
       61 tatattaaac ttctatgcat cacttttata cattaattct tatatattaa acttctatgc
      121 atcactttta tacattaatt cttatatatt aaatttctac acatcaaatt tatatacatt
      181 taaatcacat gattaactgc tttcttcaca tttctaagtg gaatagcaat tttaactaca
      241 aattttttat catcatattc aattacaaca ttaccaccat acttatttac agagtttttt
      301 acacttttta ttccaagtcc atgaagagaa ccttttttgt ctgtcaaaat atctccattt
      361 ttagtaataa tcttatttat cttagtattt tcaactctta ttaaataaaa attttgcata
      421 acatcacctt taacacaaat aactctattt ccactatcaa ttttattaca tgcctcaatt
      481 gcattgtcta aaatatttga aaatatatga catatatcca ctaattcaac agcttcacaa
      541 caactaaaat ttattcctat ttttagttct atatcattct tatcacaaac ctctttcttt
      601 tcacttaata tagcatctaa tataaaatta ccagtattaa ataacaagtc attactctga
      661 atttctacct gcaaattatt taaataagaa gtaacatcaa tacctttttt aatcatagca
      721 tttatacaca atatgtgatt tttcatatca tgacttagta atctagtttt taaatatttc
      781 ttttctatat cttcataata ctcattttca tacttcaaag tatttttaat catattgttt
      841 tctacaacta aagtattata ttgctgtaat tttttagcaa ttaaaactaa agtgatactt
      901 gatataagca atacaacaga aatcataacg aattggaaat tatgtattaa gctaccagac
      961 cctggattaa atatgtattc aaaaattaca aaaaaagatg ctatattagc gactatagga
     1021 ataactatat aaaataatgt ccttttattt atttctatat taagttttat ctttaaggct
     1081 ctatataaaa gcaaagacac aatcaaaagt atctttccca atattataga ctcaattctg
     1141 tatatatctc ccgtccccat actactcatg tcatttatag aattaagcca aactgttaaa
     1201 gatatactca aagcatctgt ggcgattagt aacatccaat aaattaagga tattaataca
     1261 gactttataa accccacatc atatgagatt ttataaaatg caatagtaat tatcatagat
     1321 ataattactc ttatatttgg aggtacatca aaaaaatata ttatgtcagc aaaaacaata
     1381 atacctacca tacaaaagtt tatttttttg ttagaaatcc ttttctcact tgtataatct
     1441 agcattttct tacaaagaaa ccattctatc ataaaggata tcgcaatgaa aatattccaa
     1501 aataagcttg actttatcat aatactttct cctaacgtat tactaaatta tttttttata
     1561 ttttatgtat aatttaatta tatacgactc tacacatgta gtaaatagat tgtcaaaatt
     1621 attccaaata agtccatatt tcttttcttt tttaattata tcactaaatt tgctaaattg
     1681 aattaaactt tttattgctt tttttaataa taactatata aatttatcta taactaggta
     1741 taaaatatat tatttataaa gtttttacat tatttctata ataaaatctt ataaatatta
     1801 caataaaata tttttttcca tgatatacct tgataaaatc tctgcttctt taagcagtaa
     1861 ctttgagttg aatcattata gtgatttata acatttatag aaatgctgtt atagatttat
     1921 atttaaacac tacaaatgga aatttaacta ttataaaaaa caatcattag ataaaatgat
     1981 tcttacaaca aaagcacttc tattgtagat ttattagtaa ataataaatc tacaatagaa
     2041 aaaactagaa gtacagtatc ataaaaatat aattaactat acctctaaat tattgttttt
     2101 aaaataaatt tttattcatt aaaaaataca cataaatatt tctagactcc gattcagcaa
     2161 acttatataa gttcactttt tatttatcat caattatttt catatacttc ttttatctca
     2221 tcttcactta agtcaaaatc attaaattta tttaatatgt catctaattt aaaaccattt
     2281 tttttcaaca ttataattat tttataatct ttttcttttg caactctaat tgctgtctct
     2341 ctttcaatct cttctttaaa atttctactt ttattcatga tataagcacc tcttttcaga
     2401 tttaataaat attcttcatt gctacctaat tcttttaatc tatcaagtgc ttttttaata
     2461 atagaatcat tcttagctat aactcttaca tctaagttag aaggattact aataagtttt
     2521 ccccatagca taattctatt tactatatag tcagatttat gaatttttgg aagctctata
     2581 aaatataatg cagttttact agaataaata tctggatgcc cgtccattgt taatctcagg
     2641 caatttacat aatcttctat gtgattatat tttacatgat ttagtatatg tattccaata
     2701 gtttttccaa tttctttata ctctttacct tcttctaact ttctaccatg tacacgacta
     2761 agataatatt cagctctttc caaataatcg ttctcaaaag ctctttgcat ctcaatattt
     2821 atatgattat tttcttcatc ttcagccaaa atatcaagtt taggtctttt ctctccaatg
     2881 taattaattg ttggatcagt ttcaactcta attattctct ttatttgagt gaacggtttg
     2941 atattcctgt tagcatatgg agcatctttt agaatatcat ttataaaatt tacaagtaaa
     3001 tcatattcat tatttaaagt gaacaatgat ttaaatacct catcatactc aggcttagca
     3061 tatccatctt cagtcacaac ttctccaaca ttttcattag tcaaaatctc ttttggaatt
     3121 aatgccttga ttttatctaa ttcatcatta gaaaaactac ttctactaag tatatcattt
     3181 aattttttgg tccattcttt atctttttct tcaattatag ttcgcttata ttcttttgat
     3241 gttttaataa tcttagattt ttcttctact ttttctatca tttcaacacc tctctttttc
     3301 ctcttttgta tttatcattt cttgaaatta aactaagtca ctacctcttt taaaagttct
     3361 tgctccataa acactttctt aaactctaat gtctgtgtat gtacttgaca ttctctctct
     3421 atctaaagtt aaataaatta atctatgtat agaggataag tccaaaaata acataaaaca
     3481 ctaattaatc atttataaag cattaattaa tcactgacaa tgctgttatt gtcaccacat
     3541 agcaccaaca tagattcttc tattataggg caattatcta tgaaaaatag actcataaga
     3601 ttagaaaatg tacttaaatt ttagttcatt taaatattca ttttgaagaa agaaatttaa
     3661 ctttagacta aaaaggaaat atttcaagag gattaagaaa ttttcttaaa gggataataa
     3721 tttttatgaa aatggaacaa atatgaaaat aaagttatga tttttacagc cataacctta
     3781 ccaacaagca cttaattatc actcaaaaaa taaactttta tttaaagaat gcacataaat
     3841 atttccatgt tcaaaacact tctatgatat atttattaat aaatctacca tacaaaaaac
     3901 tagaggtaca ttaatcataa aaatatgatt aactgtacct ctaattatac tcatcttttc
     3961 ataaatttaa tcactatgca tcaacatatc aacatattga ttaaacttat cttatataac
     4021 aatatcttag ctactaatat aatatatatt agtcttgaat aatactttct cttgattcaa
     4081 tttcttttga aatactagct ataaattcat ctaatttaat actacctatt tcacctttat
     4141 ctcttgatct aacagaaaca ttattatcag ctacttcttt ttcacctact attaacatgt
     4201 atggcacttt ttcaagctga gcttctctta tcttaaatcc aattttttca gctctatcat
     4261 ctaattcaac tcttataccc ttatcaaata actctttctt aacttcattt gcataatcca
     4321 taaatttatc agatatagga agtatcttaa cttgagttgg agataaccaa actgggaatt
     4381 taccagcata atgctctatt aatataccta taaatctttc tatactacca aaagcaaccc
     4441 tatgaatcat aactggtcta tgcttttcac catcttgacc aatataagta ttatcaaatt
     4501 gtcttggaag ctgcatatct aattggatag taccacattg ccaagttctt cctaaacaat
     4561 cttttagatg gaagtctatc ttaggaccat agaatgctcc atcaccctca ttaatagtat
     4621 atggtagacc taactcttct aaagcttctc ttaatccatt ttctgctgct tcccactctt
     4681 cgtcgcttcc catagagttc tctggtcttg tagataattc taaattatat tcaaatccaa
     4741 aagttttgta tataccatct attaagttag ctactccttt tatttcgtcc ttaatttgct
     4801 ctggtaacat gaatatatgt gaatcgtctt gagtaaatgc tctaactctc attaatcctt
     4861 gtaatgcacc agataactca tgtctatgaa ctctaccaag ctctgcaact ctcattggga
     4921 aatctctata tgaatgtagt tgagagttat agtaaattaa tccaccagga cagttcatag
     4981 gttttatagc ataatcctca tcatctattt taactgtata catattttct ttgtaatgat
     5041 accagtgtcc tgaagtctcc cataattttc tatttaatat gattggagat tctatctcaa
     5101 tatatccagc ttttctgtgt atttctctcc agaattttaa taattcattt ttaagttcca
     5161 taccttttgg taaaaacata gggaatcctg gaccttcttc aggtatcata aataatccta
     5221 attctttacc taattttctg tggtctctct ttttagcttc ttctagtaaa tgtaagtact
     5281 cttctaagtc tttgtttttc tcaaaagaag ttccatatat tctttgaagc atcttattat
     5341 tttcgtcacc acgccaatat gcacctgcta cactttgtaa tttaacagct tttacctttt
     5401 tagtagatgg taaatgtggt cctctacata aatcagtaaa atcaccttgc ttgtagaaag
     5461 atattatttc actttcaggt aaatcagaaa taagttctac cttatagtct tctccttgtt
     5521 ctttcataag ctctaaagct tcatttctag gaagttcaaa tctttctatt tttaaatctt
     5581 ctttagctat ttttttcatt tcagcttcta ttttctctag gtcctctgga actaatctat
     5641 ggtctaaatc tatatcatag tagaatccat tttctatact tggtcctata gcaagctttg
     5701 cttctgggta aagtcttttt atagcttgag ctaatatatg cgctgatgta tgtctaaaaa
     5761 cttccttacc ttctttatct tcaaacttaa ataaattcaa gtcacaatca gtatctataa
     5821 tgtggtccag acctacaacc tctccgttca cagacgctgc taccacattt ctagctaatc
     5881 cttcactaat ggatttagct acatccataa cagaaatagc attttcaaat tcttttattg
     5941 agccatcttt taaagcaact ttaatcattg ttttttcctc cttattgaaa taatttttag
     6001 attttttgta ataaaaaatc gccccaaata tgtttgggac gatattaatt cgcggttcca
     6061 cccaagttgg cgtatacaag tatatacaac cctcttgatg actataaggt cgtcacccga
     6121 gcactttcac gtatagtata aatacaaata ctgctaataa gctcagctcc aaggtagttt
     6181 tcaaatagac ctattacatg ggctctcagc catagaccca aattctctga gaatggtttc
     6241 tactctactc ttcctcttca acactatgat ttttaatata ttttcattat aacagatagt
     6301 tatacatcaa tcaacattaa tgtaatatat taattaccca ttatttttca tctcaatcag
     6361 ctgtaaacca atattcttat atttttatat taaattaatc tcttgattta tagttatatt
     6421 ataatatttt cataaattta aaatttttaa aacgtatcca catgcatgat agatatgact
     6481 agataaatac taaaactaat ttttattaaa actaacttct aatttttgta ctctttttag
     6541 gttttgtata cttaaataca atcaatactt aatatggtta ataagtagta cagtaaatac
     6601 ttaaatacta ttaataagta acacaatcaa ataaataaat acaattaata tctaaatgct
     6661 attaataagt aattcactca aataggtaaa tacaattaat atctaaatac tattaataag
     6721 taattcactc aaataggtaa atacaattaa tatctaaatg ctattaataa gtaatttact
     6781 caaataggta aatacaatta atatctaaat gttattaata agtaattcac tcaaataggt
     6841 aaatataatt agtacttaaa taaatgctac ttctcatata tttaaataaa catagtaatt
     6901 aacccttgag ctatacctat taaaaaccct aagacaagcc ccaaaaactc tatatgtttt
     6961 aattctttct tagctattct tattattatc tcttccaatt catataaatc tagttcatta
     7021 atcttatctt cgaccatttt ttgtatatct atacgttcat ttgctttatt tattatattt
     7081 ttactcaatt catctatact ttgcttaact tcagactcaa ttatctctcc tagataatct
     7141 tgtatcatgt tttttatacc actcgggata aatgaaactt tctcatgtat tataattttt
     7201 acccttgctt ttatgtatct tacaacttct tccttatctt cgtcagtaat gatatttgca
     7261 agtatttcgt ccatagaaag aaattcttct tgaattattt ctcctacatt tgctgctatt
     7321 tctgctcttc tctttggtat aagacctact atttctatat ttaaaatagg tattttaata
     7381 ggctctattg gtctaaatat cagtcttata gcaactacat tcgtaacata acctataaat
     7441 ccaccaataa cagctaatat cagtatccta attaaattat ccattataaa attactcctt
     7501 atcaattgta tatttgccag ctatattgca ttaatcaagt agatattctt tatacttaat
     7561 aactctagtt atactatagc tttatataca attataagtc ttttgtgtcg atttaaaaag
     7621 aattttttta attttatatg gatagctttt atatgcttag attctagttt tccataatta
     7681 tactaacttt attttcaaaa attgcttcta cagtatctat aatttccttt gagcaattat
     7741 tatttaacaa atccaatatt tctatttttt tgggacatag agttaataag gtacttatta
     7801 aaaagtcctc ataatttaaa ttttctctta ttaccatatt tataatttct tcatcatcta
     7861 tactgtctat cttatttcca tttttatcgt ataaaacaaa tgtattatct tccattatat
     7921 gaactttaag caaatcaatc ttttcttctt gagtatctat aaagtatttt aaaactctta
     7981 taaattcatc ttggtctttc tttattaaat actcctcaac tgcaatatca cctatagcag
     8041 aaatatattt cataaattct ctcattctaa atctaataaa accttctaca tttataaaat
     8101 cattttcttc tatataattt ctcaccctag taaatactgt ttctctcata aaaatctctt
     8161 ttttagcaaa tatgttcagt gaatatatgt atatattatt tttatcttca ggatatgtac
     8221 gcccatagca ttttagaatg tattctttca aaagtttctc tttcattata cttattatca
     8281 tactagttat ttccgttgat atctcatcaa tatttaaagc tctttgttca gctttagtac
     8341 cattaagtat ttttttattt aacacttctt gctttgcaga atctatcact acttctacaa
     8401 tgtcattcgt atatattaat ctatcgttta cattattatt cctcaaaaat ttcatggaag
     8461 aaatattatc cttctcaacc aaagttatcg ctcctttctt gtcatgagag gaactataga
     8521 acctatatct agtaaatttt cacagctaag tgtaactatc tccttgtttt tactttctac
     8581 aaaacttttt atacgttcat agtcaggatg ttttttaaca tctccacaaa aagctagttt
     8641 atccttagaa atcaaccctg tacatccacc tataaatcca taattcatct caaataaatt
     8701 tatgtgacct ttttctatca ataaacactc tatttctgta ttcttcatac ttttccaaat
     8761 acctttatct gatgttatta tagacgttct atctactata cagattgagc atttagaata
     8821 gccctgctct atatttatct ttttaagttt attattttca atataatcta atattgcttt
     8881 gtcagtatac ttaaaattgt gtatagcata gtcaccaact atcgcaatat tgtaaggtat
     8941 atcataaggg tatctattta ataaattagc ttctccctta attatattaa atttatatct
     9001 acttaataac tctttgtatt gattatatac attcggcgca acaattatat ctccttttcc
     9061 taaattacaa atacatatat ccgggtgata ttttatgctt tcatataatt ctttacattc
     9121 tattgttttt attatctcta tatttcttaa ttttaattca ttttccatta gataactaat
     9181 tcttttgtcc accaaagcta gacacaaatc gtcatctagt ataaatggtt ctcttgtaaa
     9241 ttccattttt tactcctttt tctttttttt gataaatttt ttatgtgaat ataaataaag
     9301 tgtgtagcag aatttttctg cccacacact tattgttata attttatata gttattctaa
     9361 gcttaattag tatagctttg tacagttggt tcaaatttaa taaatatatc tttttatcac
     9421 taatctaaac ttaagcagta tattccgtac agttagcaca aatttaataa atatatcttt
     9481 ttattgctaa tctaaactca atcaatatat tttgtacagt tagactaaat ttaataaata
     9541 tatcctttta tcaccaatct aaacttaagc agtatattcc atactgttaa cacaaattta
     9601 ataaatatat ctttttatca ctaatctaaa cttaatcaat atattttgta cagttagcac
     9661 aaatttaata aatatatctt tttattgcta atctaaactt aagcaatata tttttataat
     9721 cttctacatc aataattaat atgtatcttt cactttgtta tactcttttt ttatgtattc
     9781 caaaacatct ggtggtatat ttctactgct ctttgaagtc aagccaagtt ccccatcttt
     9841 tattttaaat tccattactt tacttccctt aaaaccatct tttgcaacgt tctttatcag
     9901 ttcatataca ggttgttcta catcttttat aattgaactt attacattat ctggagccat
     9961 atcgtattgg tctttattaa tagctataac tttaccacca ttttctcttg ctacctgaat
    10021 agctctttta ctatcatctt ctgtagctga aaatacaaca tctctatcag aatctctcat
    10081 ttcctttgca atagttctag ctgattcagg gttttcaaat atatttgaat acttaactag
    10141 taactcagca tctttatttg aagttttaag cccctttcta aatccagatt catacttatc
    10201 tctactagaa ccctcaactc ctcctataaa accgactttc ccagtatcag tcattttacc
    10261 tgcaataagt cctgctaaat atccagtaac attatcttga aatgatacag aaattacatt
    10321 agctggctga actttataag tatagtcaac tattgcaaat tgctgtttgg gatatttagt
    10381 agcagcttct ttaattggct ctgcaaatct ggctccaact gctattataa aatctggttc
    10441 atacttatgc agtttttcta tactctcttt aaaactttta gaagtctttg gtttttcaag
    10501 ttttatccta acatcaaaat cttcgtcagc cctttttaag gctgatagta cattgttatt
    10561 atatgattcg ttatttaagt tttcggtatc taacaatact ccaatagttt ttcttacata
    10621 tgaatcatct tctgtgtcat cttctttatt cttactttta ctatttgatt tatccttttc
    10681 tgctgaagtt agcaaacttt tagactcact ctgtttcgat ttagcactat ctatattaga
    10741 attattctta tttactttat caaacccaaa atcaaaatta catatagcta atataaaaat
    10801 tgtaaaaact actacaaagc aagccatcaa tatccttttt attttcacaa aattcactcc
    10861 ctgatttaga taggtctgac ctacctctgc atatgtcttt ctatatatgt ctattataac
    10921 atccaattca cctcataact aaactcataa gtattcttgg atattatagt agattattta
    10981 ttgttcttat taacatattc taacacatct ggaggaatat tttgagaact attttgggca
    11041 agtccagtac ttccagactt aactgtgttt tctataacct ttccgccttt ataatttcct
    11101 tttacaaaac tttgtatcag attataagta ggattctcaa aatcttttac aatagaactt
    11161 ataacatttt ctggagctaa ctcatgttgg tctttattcg ttgctataac ttttttattt
    11221 tttgccctta ctgcatctat tacagctttt gaatcatctt cagtagtaga aaaaataata
    11281 tccactccat tatccatcat ttttttagct atagattcta ctgatttgct atctttaaaa
    11341 acatctgcat actcaactga aatatccttt atactactat tagaaaattt aacaccctct
    11401 ctaaaacctg attcaaattt atctctacta gaactctttg ttcctcccat aaaaccaacc
    11461 ttatcgcttt cagtcatttt tccagcaatt aatccagcta ggtatccaga tttattatct
    11521 tcataagaaa ttgaagttat attagaaggt tgcttgtcat actcatagtc tataattgca
    11581 aattgttgct ttggatattt cttggctacc atctccaatg gttttgcaaa tctagctcct
    11641 acagctataa ttaaatcgta attgtcatta cataaaatat ctatactatt agaaaaagtt
    11701 gaggaatcat ctgattccac tttgtttgta tctatattaa gtttcttttg agcattgttt
    11761 aatgctaata aagctgaatt attcatagct tcattatttg ttccttcaat atctagtatc
    11821 attgatacag atttttttga attactttta tcgcttatat cacttttatt atctttgcta
    11881 gagctaggat tattacttgg gttattagca cacccaacca acattccagc tatcattgct
    11941 acagttaaca tcaatatcaa tattcttttg aaacgcatta aatttcactc ctcatacatt
    12001 tacgatattt ttatatccta tttactttta catcctattt ataatttttt tatatacaat
    12061 ttacaagtac attttgaaat ttttttaata aatcttacta taaaaaagtg tgcaatagat
    12121 tcttatctat ttacacactt ccctacaaac taaatctaat atatcaacac ttaatttcat
    12181 ccaaaataaa tccaatttaa catctcaaca attaatattc tatcctaatt taataaaaaa
    12241 ataaataatg tcatccaaaa atatattatt tattttttaa gcatatgata ccagtaacac
    12301 caggaccagc atgtgcacct atacacacac caatttggaa aaatctaatt tctttaggtc
    12361 ttaattcatc ttgcataacc ttagataatt gttctttttc cttaaaatca tcactatatc
    12421 ctatatacac aatttggtct gataaatcat caccacaatt tgattttatc aattccatca
    12481 tcttagaaat tacattcttc tttcctctta cttgagatat ttgcgaaact aatccatctc
    12541 ttatttcaag aatcggtttt atatttaata tacttcctat tgctgcctta gtagatgata
    12601 ttctacctcc cttttgtaaa tattccaatg tatctactga aaatataaca aaagccttct
    12661 cttttaattc atccaaaaca ggcaatattt cagcaacaga ctttccttca cttgcaagtt
    12721 ctcctgcttt tattacaagt aaaccagttc ccatacaaag caagttagag tcatatgtat
    12781 atattttacc atctacatca ttctttgcca ttacagcact ttgatacgta cctgtagctg
    12841 tggcagaccc tgctatataa agtatctctc tatcctcatt tgtatatttc tcaaaaattt
    12901 ccttaaattg ggcataagta gcttgagaag tttttggata tacattttct tctctaagcc
    12961 ttttataaaa ttcatctcca gatatatcaa ttctatcctt gtattctctt tcctccagta
    13021 taactgttaa tgaaactata tctatatcat atttatctaa caaatctttt ggcacgtccg
    13081 ataaactgtc acagattatc tttagatttt tcattaagta tgctcctttc tcatttaggc
    13141 ttgttgaaac tacctcatat ttgggtatcc tatttgtctt aaagcctcgt acactacaat
    13201 agcaactgaa tttgataaat ttaatgacct agctttttca acatccaaca taggcactct
    13261 aatacaagta tccatatttg ctttcaacaa aggttctggt aatccctttg tttctttccc
    13321 aaatactata aaacaatttt cttcatattt cacatctgaa tgtgactgtt tagcctttgt
    13381 tgtagaaaaa aagtatttac tatttggata cttttcttgt aattcttcaa agctatcgta
    13441 atattgtata tcagctatat cccaatagtc aagcccacat ctttttaggt gtttatcatc
    13501 caatgaaaaa cccagtggtt ttactaaatg taaagttgaa cctgttgctg cacaggttct
    13561 tattatattt cctgtatttt gaggtatttc tggttctact aatactatat ttaatgacat
    13621 tttaatcctc cctaagtatc gcttttattt atcaaatttt attttataaa cagaataaca
    13681 ataatattat actattatat aaaataatgt taataataat ttttatttca tgtatataat
    13741 agttttttat ctaacatttt cacaagtatt attatatcag aaaatccctc ttgtacaact
    13801 tactttctaa aagttatttc gtttcattaa gttctttata ataattacaa tcttctttta
    13861 ttgggcaact tgcacattct ggatttctag ctttacacat tcttcttcca tgaaaaatta
    13921 atacatgatg tgagtgtgac catctttctt ttggtatagc ttccataagt gcaaattctg
    13981 ttttttgagg atttggctcg tccactattc ctattctatt gctaactcta aatacatgag
    14041 tatctacagc tattgctgga tgattaaaag cgttactaag tacaacccct gccgtttttc
    14101 ttccaacacc aggtaatttt ataagctttt ctaagctatc aggtacttca ccatcatata
    14161 attcacatag ctgttcagat gtatccttta ttttttgaga cttgcttttg taaagaccac
    14221 aactctttat ctctttacta atttcttcaa tgctcaaatt agcaaagtct ctagctgtgt
    14281 tgtacttttt aaacaactct gaagtaactt tattcactct tacatcagta cattgtgctg
    14341 ataaaatagt tgctattaaa agttcaaagg ctgtcccata atttaactca catttagcat
    14401 ctggatacaa tttttctaac tcgtctaaaa tcttatttac gtctttttca tttgctgact
    14461 catttttaat tttagactta cttttagttt ctttacttgt tttttgtgta gccttattag
    14521 aactactctt ctttgttttt ttctccgttt cacctttttt agtacttttt ttacttactt
    14581 tgatactttt tttatcttct tcaatatcca taaaattctc tatatctttc tcatcaatta
    14641 tctcatcttt tttaccaatc attttatcta ttcctttttc atttatctct tccttctcaa
    14701 ttatatcttt cttattcatt ccttttttat ttatctcttc cttctcaatt atatctttct
    14761 tattcattcc ttttttattt atctcttcct tctcaattat atctttctta tctattcctt
    14821 ttttatttat ctcttccttc tcaattatat ctttcttatc tattcctttt ttatttatct
    14881 cttccttctc aattatattt ttcttatcca ttcctttttt atttatctct tcttccaaaa
    14941 ttacatcttt ttcatttatc tcttttagat ttacattttt cataattatc ccccttcaaa
    15001 ataccaattt ataaaacgct atttataaat agtttaatca tatcaacaaa aaaataaaca
    15061 tcaatatata aaacatacaa aataagaatt taatttatat atatttacaa aaaaagcaaa
    15121 aacctattaa agttcttgct ttttattata cataaatacc cattaaattc tataataaac
    15181 ttattctttt gacacagttt gtatcttgct gaaagatatt aatttttctt ttaccaattt
    15241 ctttatttct tcaaatttaa catctgtcaa tatctctata gcctcttcta ctctttcaac
    15301 agtatatatc ttaaattttc catctcgcac tgcatcatta acttcatctg ataaaacgag
    15361 atttctttga ttttttctag gaattatgac gccttgctca ccatttaacc ctttagtttt
    15421 acatatagaa taaaatcctt ctatcttttc acttatacca ccaactactt gtatatcacc
    15481 cttttgattt atagaacctg taacagcaat attttgttta attgatactc cactaagaga
    15541 tgataatagt acatataatt ctgcagaaga tgcactatct ccatctattc caccataatt
    15601 ttgttcaaaa caaacatatg catttagtga caatagtaaa tcctgtgcaa aattctcaga
    15661 aagatagcca cctaatataa gtactccttt gttatgtata gagccactca tatttacttc
    15721 tctttctata tttactatgc ctttacttcc tgggctagtg gtcgctgtaa tccttgaagc
    15781 tctaccaaaa gagtattctc ctgtacttaa gactgacaaa ccatttatca ctcctacttt
    15841 tcttccctct gtttctatta gagtaaatcc attttcaatt gattcatcca tatgtgtttc
    15901 cactctattg attctttttc tcttttcact tattgctttt ttaacatcac actgctctgt
    15961 atactccgaa tttctaaact ctgcataagc acttccttct attacaactt ccattatttt
    16021 attaaactta gttgataatt tttctaaatc accagataat ctagtgctaa atttaataac
    16081 ctcttcaact gctgatttag taaaatgcct taaattattc ttatcacatt ggtatgctat
    16141 aaatcttgct attccttctt cattatcttc atttttatcc atttcactat caaagtctac
    16201 aaatacttta aaatattttt caaaatcttc atcatatcta tataaaatat catatatata
    16261 gtggcttcca atcagtacta atttaacatc caatggcata ttttctggtt taattgctgt
    16321 ttgtgtttcc aatattactt ttttagtctg caaagtcttt ttaagaattt cccaagacat
    16381 tgtatatttt agtaattggt ctgcataaag tactaaataa ccaccattag ccctatgaaa
    16441 tgctcctgat aatagttttg tgaaatcagt ctttatattt ccatttgcat aatcatactc
    16501 tgcctttcca aatagatttg ctggacttgg gtttatctct actacaacag gtgctgattc
    16561 tttattcttt gagcttccat tgtctacaaa taggttaatt ttatatttaa taaaatgctc
    16621 tttatcatac ttatctttta actcctcttc atctaaatag aataaataca tatattctat
    16681 tatatctttc tttatatttt ccagataagt ttgaatctta tcataatttt catatttttc
    16741 acacaatctc ttgatatgag gttctactac aagtttagct atctcttctt caagctctaa
    16801 cactgctttt tcagctattt cttctaagtt tctaatttta tatacgacct gtatcgccat
    16861 attttctagt tctcttttag ctttgtaaaa ctcttcatca gactcatcac tttcatcttc
    16921 ctcttctttc aaagggataa aaactattcc cattttactc tgttttaatt taaatccttt
    16981 ttcttctcca tattttttaa tttgcttcaa aagcatatct ttttctattt catattcatc
    17041 cagaagttta tttttttcta tttcaaaatt ttcactttca aacgcatttt taaaatcttc
    17101 taaaagactt tcaattaatt tctccatatc tttcttaagt tctcttccaa accccttttc
    17161 taattcaact ataataggct ctctagggtt ttcaaaatta tatatataac accaatcttt
    17221 atgcgtattt tttttggatg catactcatt taaaaccttt aaggcataag tacttttacc
    17281 agttccaggt tcaccagcta tatatatgtt gtacgctgga ttatttattt tcaaccctaa
    17341 ttccatagct tctacagctc tttcttgacc caatatataa tctagtggat ttatttcaga
    17401 tgtattatta tatttcaata ttcccccacc tccccatatt taactttaat gatataatat
    17461 gacttttaca caacaagtgt taaagtaaat acacattaaa gtatatgaaa cagactctta
    17521 ttattagtca tcatacttat ctaaaattat ttccatattt ctctttatta tatttcttcc
    17581 tctccagcta aaagctctat ctccatattt ccttttaaat tctttatttg aaatattttg
    17641 tatctcatca taattcaaat aatctattat attttcttta aattccaaaa tatgagttgt
    17701 tggtatttca acattatgtg gacatacttc ttgacaaata tcgcatccaa ataccttttt
    17761 gccattcttt aaaacaactt tttctttctc tgacaaatct cctttttttt gagttatata
    17821 tgataaacat tttttggggt tcatatcata atttcccaat atagcatttc ccggacaata
    17881 ttttacacac tttccacatt taaaacaact tttttctagt ggtttatctt cttcaaattt
    17941 ataattattt actatatacc ctataaatac gtaagaacca tattcatcag ttatgatatt
    18001 attatttatt ccaaaatatc ctattcctga taaataagct aaatacctgt caactaatgg
    18061 tccattatca gcaaataact tgtatttaaa atctgttact gttttagaaa tataatcaca
    18121 aatttgttgt aaaaagtcct ttactacaat gtgataatcc tttccatgac aatattttga
    18181 taaatttgaa tttttattct tttcttgtct atttatatga tatggaaatg cacagactat
    18241 tatagagtta gcatccttca ttatatgtct tgggttaatt ctgttctcaa ttattggttc
    18301 ttccatacca gttaaataac ctttttgttg tctgtcctta agtatctttt ctaaattatc
    18361 atacttatct acccctgcta ctcctacaca gtttaatcct attaaattac aaaatgcttt
    18421 taattcctta ttatccataa aattattcct ttttaatcta attgactttt taaatttatt
    18481 taattttttt agtcttttta accttttcta gctactaact cttttaaata cttaatcttt
    18541 ttagctattc aattttctta cctacttaaa tcttttagat acttcgtctt tttacctatt
    18601 taatttttta cctactaacg cttttagata cttaatcttt ttatctattt agttttttac
    18661 ctactaactc ttttagatac ttcatctttt ttatttacta actcttttag atacttcgtc
    18721 tttttcattt acttatttaa cttttttatc tacttaactc ctttaaataa ttagtctttt
    18781 tatctattta attattttac ctactaactc ttttgaatac ttagtctttt ttatttattt
    18841 aattttttta tctactaacg cttttagata cttaatcttt ttatctattt aattttttta
    18901 tctactaact cctttatata cttagccttt ttcatttact tatttaacta ctaactcttt
    18961 tagatactta gcctttttca tttacttatt taactttttt acctacttaa ctcttttaga
    19021 tacttagcct ttttaatcta ttcaaccttt tttacccact taaccttctt accttttaat
    19081 ataaactcta aaacttcaaa tatcattagt tatattttga cattctttct atagttaagc
    19141 aaaaagattg attttttctt ataattaata tcccatttac attgccattt attaaatctt
    19201 tacttgactt atatggattt ctttttactc tctttactaa actatactta ttaatattat
    19261 gaaggtctta taatagacta atatgaatat gtccatcata tatcaaattt taaaatttgt
    19321 aagcaaaata cttatcttat attactacat ctaaagtgga aaattttaca aaaaagctct
    19381 gtaaaaacac tttaaataac aatttgcttt taaatcatat acagattata ataaatactg
    19441 tataaggatg gtgaaaaatt tgattaaaat aacaattcca ggtagaccta ttagtaaatc
    19501 taattttaag cttcataaca taaatggtca agcatggatg ccttcaaagg gaaaatattc
    19561 taagtatctt gcatacgaaa acatgattgc aggttttata aaccaacagt atcaagggga
    19621 aactgtcgaa gaaaatctaa taacagtctt gaaattattc ttcccaaata agagaatggg
    19681 ggatttacat aattatccaa aaagtatttg tgatggtatt gaaaaaagtg gaataataaa
    19741 aaatgataaa caacttaagc cagtactttt atttgatttt atagataaag ataaccctag
    19801 ggttgaaatt gaactttatc cagtatctaa atacgatata tcctataata tttttgaaaa
    19861 ataaagattt tatgtgttat tcgacattat attgatactc cagtatttga aactatacta
    19921 atacaacaat aataattgtg ttatgtctat actcaaacaa atgtttttaa aaagcattaa
    19981 gtaaatattt aataagtatt aaacaaatat taaatttata taatagctat aactatgtaa
    20041 atttaatatt tgtatataag accacatcta ataaaaagaa atcggagtaa atttaataca
    20101 ccgatttcaa taaataaaat ctatcctagc ttttcaacaa cagtcttaag catttctctt
    20161 ataggaagtt catatggaca tctactctcg caaagcccac agtcaataca ctcagtaggt
    20221 ttaacatcca taacctcata tttttcttta gcccactctt ttaatccata tctagtataa
    20281 taaccttcac ataaaaaact caatggtata tttattccta cagcacaagg catacaatat
    20341 tcgcatcttc tacagaattt tttacctaaa gaatttctta tttcttcaat ttccttatta
    20401 tctttttcat ctagcacaag attttcaagt acagcgacat tttgtctaac ctgttcaaca
    20461 ctctccatac ctggtataac tacatctatg tactctttag atagtatata tttaatagcc
    20521 agtgttgcat tgtctaaagc acctccagct aaaggtttca ttactattat cccaattcct
    20581 ttttcatgag cttttttaaa cacttcatct gcttgtcctt caactatatt ataagggaat
    20641 tgtatagtgt caaattttcc atcttctata gctttttcta tagtatttaa attgtgacta
    20701 gttataccaa tatgttttat cttaccttgc tcctttgcct ctaaaagggc actataagcc
    20761 attttatctt taaaaagatt atcatactcc tcttctttaa cattatggaa ttgatataaa
    20821 tctataaaat cagttttaaa attatttaaa cttatttcaa catctctctt cattgaatca
    20881 taatctctgg acatactttt agtagctaag aaaaatttat ctctttttcc ttcaatggca
    20941 attccgattg cttcctcact tattgtatac cctcttgcac tgtcaataaa gtttattcct
    21001 tgtttttcta actcatttat aactaagtta gtatcatctt gagtaatcct ttgtattgga
    21061 atgcccccaa agccaactct ctttattttc atgttagtat tgcctaaatt tctcatatct
    21121 ttccccacct aacttttaat aattttttat ataatataaa tatgtcacct ttaattatac
    21181 aacaaattat gagaatcttt gcaatgtatt tacatactat tcataatata attatatttt
    21241 atatcagtct attcataata taatcatact ttatacccag ttaatttgta tacaattttc
    21301 gttattccta tccattaatt taaattatca catttcatga ttgtatctcc accctaatta
    21361 tgttgagggt ggagtatttc acatattttt aataaaggtt tatttactag atagtatttc
    21421 ttttatcatt ttaagctctc tcttgtgacc ttcatcatca aaaggagttt ctaagaaaaa
    21481 tggtaaatgt tttagactag gatgttccat aaaatcaatt aatgctttta atcctatctt
    21541 tccttcccct ataggagcat gtctgtcttt tttgtctcca aatggcatca tactatcatt
    21601 tagatggaca gttttcaatc tttctatgcc tataacctta tcaaattctt ccaaaacccc
    21661 atctaaatca ttcactatat cataaccagc tgaaaaaata tgacatgtat caagacacac
    21721 acctattttt tctttatgct caattttgtc tattatactt tttaattgct caaatttaaa
    21781 acctatttca gttccctttc ccgacatagt ttcaagaagt atagttatat tttcatcacc
    21841 ctttatggca ttgttcaatc cttttactat tctatctatg ccaaaatcta caccaccacc
    21901 tacatgactt ccaggatgaa aacacatgta ttcaattcct aatgagtcca tccttcttat
    21961 atcctcatct atcacccttg ttgcaaattc gtatacatca tcctttgtac cacccagatt
    22021 catagtatat ggtgcatgtg caagcagtgg tgcaaaatta ttttcttttc taatttcttg
    22081 aaatttaatt atatcttttt catcaaattc tttagcattt cctcctcttg gatttctact
    22141 aaaaaattga aatgtatttg caccaatata tacagctgtc tcagccgcat tagcaaatcc
    22201 ttttgctatt gaaagatgtg ttcctaattt aatcatatta atctcctttt atttgtacta
    22261 ctacttgtac tcaattttaa ttttgaacta ctccacctta actacattta gccaacatat
    22321 gaagatggag attcctattt gatagtactt tttcactaaa aattcgtaag ctctaaggga
    22381 tgttctatcc caactaatct cattcctctt ttaacatatt tctagcattg atttaattta
    22441 tattaatttt taattttaat atttacactt taattatacc cattttaatc aatttcaaat
    22501 attataatct tacatacttt gtatattatc taagcacttc ttagatagtg tatacatctt
    22561 acactataca tattatttac aaatacaata ggtataaaaa aacaaaaaaa tagagattaa
    22621 aaatcattgt tttacccata ccaaaaacta ttttttgatt attaagtaaa ttttgatgtt
    22681 caatctctaa tattatatct ttaattaatc cataattaat tctctaacta ggacattcct
    22741 cctcaagagt aggaactata accatagcta tatcatactt agaaccttca acattgtgtt
    22801 gtcttttatt taaaatttca tctatctttg acttcatagt gtcactaagt tcaactaatt
    22861 catcatgagt aagagaaata tcatgataat caactatatt ttcattttct aaaacatctt
    22921 cctctgctgc cttataaaaa acttcactca tattttcaaa ttttgatatg tacaattctt
    22981 ctttttcttc cccattgtct aaagaaaaat taagtatcct attccctata accacatttt
    23041 ttgcacttgg ataatagtac ttttccacaa tccccgactt tattttttcc tcaactaatt
    23101 ctaaaatacc aactttgtat aacattttta tatggtagtt tatttttgca tgaggttctt
    23161 ccaataattg agataattgt tttgctgaca atggcgattt gtcaaatgct tttagaatat
    23221 ctattctacg tggatgcgca atagccttta tgcactccaa atctcgtaat accaatacct
    23281 ctttcattga cccctcctaa aaaatttatt taattcatat ttctttacaa tttaattata
    23341 tattaaatta tatgttgttg tcaaaaaaat cctttcttaa tattttaaaa aatatttaat
    23401 ttgagtttat ttgacaactc ctctaaaact ttaactcctg cgacgctgtt accttttttg
    23461 tcaagcgcag gtccataaac accaatcccc atgcgtcccg gcacagaagc cattatacca
    23521 cccccaactc cagattttgc aggaatgcca actttaatag caaattcccc agaagcatca
    23581 tacattccac aagtcatcat aaatgtcttg acaatcctag aaaccatttc actcattaaa
    23641 tgctcaccat tttctatatc tactccataa tttgcaagat ttattccaat tctcgctaaa
    23701 tctacagaat ctatctcaat tgagcattgt ttaaaataaa ggtctagaac atcttctaca
    23761 ttccctctga taaatccatc acttttcaat aagtaagcca tagctctgtt tctatctcca
    23821 gtcatttttt cagacttata gacatcatag tttattccta tattatcatt ttttgctaac
    23881 tttctaaaga aactaagcat tctttcttct ttttcttcta aacaactccc atttataagg
    23941 gaagtggtta ctatagcccc tgcatttatc attggattac agggtttttt tgtgtcatta
    24001 atttcaagtt tcattattga attaaatggg tccccacttg gctccattcc aacgtttgaa
    24061 aaaacgtctt cccaatcatt atccataagt gccatagcaa gcacaattgg ttttgaaata
    24121 ctttgtatag taaactttgt gctacagttt ccagcactat aaagattatt ttcgctatct
    24181 atgatacaaa ttcctaaatc atttcttcta gcgtttttta attctggtat gtaactagcg
    24241 acttgtccat aattagtata cttcttatta gaacttataa tttcttttaa taatgtctca
    24301 tctaacgtat tcatatgcac ctcttatatt atgttatata ttctatttta gcacaagttt
    24361 atactataat aaaacaatgt taaaaaaaat tatactttaa aattattttc tacaagaatc
    24421 aattcatttt tcaaacaatt taaatagttt aaatagctta ataatttaat aatttaacaa
    24481 cctagcaatt tagtaatcta ataatccagt aatttaacaa cctaataatt tagcaatcta
    24541 ataatctaac tatctaataa tccagtaact taacaaccta gtaatttagt aattcaacaa
    24601 tctaacaatt caataattta acaatttaac aacctagtaa tctaactatc taataatcca
    24661 gtaacttaac aacctagtaa tttagtaatt caacaatcta ataattcaat aatttaacaa
    24721 cctagtaatc taactatcta aataatccag taacttaaca acctagtaat ttagtaattc
    24781 aacaacctaa taattcaata atttaacaac ctagtaatct aactatctaa ataatccagt
    24841 aacttaacaa cctagtaatt tagtaattca acaacctaat aattcaataa tttaacaacc
    24901 tagtaattta gcaatccagc aatctaataa tccagtaatt taaattttta taacaccaaa
    24961 aatttctaac ccaacacctt ttactctttt actcttttac tcttttactc ttttactctt
    25021 ttactctttt actcttttaa aaaataatca cataaattaa caaaatatta aacaaaaaag
    25081 taaaatttaa aataaaaagc ttgcttcata caaaagtata aaacaagcct ttttatatat
    25141 caactcaaac tttaaaatct gtatcaattt aattctccac tttacactta atatacttaa
    25201 cataaaccaa taacactact tgctatgcca tttaatcttc ataaaacaat ctataataca
    25261 cacattaatc aactatttta cttcctccaa taaattccct aagaattgaa atacctggta
    25321 tgtcagaagt atcgtccaat tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt
    25381 caatatgtgc aggattatct tcctttatct cttccaacaa aggtttcgca aatcgtttta
    25441 aaactgccaa ttcatataca caagcagagt taaatatagc atctgcctcc tcttggtacg
    25501 ggaatatatt cttcttttct cctcttctaa ctgatgccca ttgtaaaata gtattatcag
    25561 cactatatcc tctaaaatta tagtctctaa caattcttct tatcattctc aaatcagcag
    25621 tagggattcg attatggtca tccaaattta tttgagtaag ggcacttatg tatatcttaa
    25681 atttatcttc atctggtata gatgatgtta aaataggatt tagcccatgt ataccttcta
    25741 atataattgg ctgatttgaa tctatcttta tctttttacc agtgtattct ctcttacctt
    25801 ctttaaaatt gaatcttata tcatcaattt cttcaccagc taaaagtttc tttaagtcta
    25861 agttaaactt ctctaaatct attgcataaa ttgattcaaa atcataattt ccatattcat
    25921 ctaaaggagt atgttttcta tctataaaat aatcatctaa agaaattgaa ataggattta
    25981 aattatttac tctaagatgt attgataatc tgtgagcaaa acttgttttt ccagatgaag
    26041 atggagctgc tattaaaact actctcttat tgccatcctt tatcatatcc gctatttggg
    26101 ataacttctt ttcatgcaat gcttcaaatg tccttattat atctccatat tcattgtttt
    26161 ctattatttt attaagagat actaccttgt caattcccat aagtcttgac cattcttctg
    26221 cttcataata tacacttgat agcttaggtt gaggtacaaa tttcatagga agagtcttat
    26281 cttcttcact aggtcctaaa attataacgc ctccattata aagcttaata tcaaatgatt
    26341 ttatataacc agttgatggc aacatataac cataaaaatg gtctatatag tttctacact
    26401 tatatacttt agcactatca tactctttgt atttcaaaag ctctgccttt tcatacattt
    26461 cattattttc aaagatagaa atagcttcat ttcttgtaac ttcaattttt tctataacat
    26521 aatcgttgtt tactatctct ttcatcttgt tttttatatt ttcaacatct gcttctttaa
    26581 gctttctatc tatatgaact tcacaatata gcccatctga tagtgaatgt tctattgtaa
    26641 ctctgctatc ccaaaagatt tctctacatg ccattacaaa tataaatgac atgactctaa
    26701 agtaaactct ctctccatct tcattagtag tatctaaaaa atttatctca caatccttct
    26761 taactctaca atttagctct tttagtttat tgtctacaac tgcaagtgtt atataaccct
    26821 tatgctcatc acaaaattct tttgctatat ctcccaatct gattccaggg gaattttctt
    26881 ctaatgaata ttccttatct tttccttcta tttttaagtt tatttttttc acttcaacat
    26941 ctccttcctt ttgatgaata taaattttaa ttaatgaggc atatcaacaa gataaagaga
    27001 ggtatttaac cctctcctac aatattattc aatttactta tagtatcttc aatgtcttcg
    27061 taatttattt ctaatactaa atattctgca tctaatgtta agatctcttt aatcccctct
    27121 tcagataaaa ttcctcttcc tataccaaca tgtgtatcat tgataccatc attatcactt
    27181 aagtgtatca gtcttatttt atcctttaag ttctttacat aagcatcctc atcaattaaa
    27241 cagtgtccag tatcgtaaca aaaacaaatc ttagaatttt taatcctctt aaatatatat
    27301 tcaaaatcat aagatatatt tccaatattt gaaaaatccc caccttttga ataagtattc
    27361 tctatacata catatgaatt tgtatttaat ttatctaaaa aatccacact atttcctaaa
    27421 tatttgtctc tattctttct aactctattt gtcataacat atcccaaatg aaggttaaaa
    27481 taccttaaat caaatccttt tagttcaaat tctatcttct caataaaatt tatccatgaa
    27541 tttcgtatat actctatgtt ttcgcaagta tttaattcca taggcaaatg tatccctata
    27601 gataatccta acttagaaat tctctcttta tatttacata actcactaca ttcagatata
    27661 ttgtcaattc caatctctaa atgagttatt tgctttatac tctcacaaat gtttaaagcc
    27721 tcctcaatat taaacaataa ggacgctata ccaactgcca tacaaatcac ctactcaaaa
    27781 atacctatat ctgtaaaagg aaatactctt aaaataactt ttcctttaat attttcttca
    27841 tctactaatc ccacttcttt atatctactg tctaaactaa cttctctatt gtctcccatc
    27901 gcaaatactt ttcccttagg aatcaccata tcaatatcgc cttcagtata attatcatgt
    27961 atatatgaaa cttcatttaa taacttacca tttatataaa ccttagaatc cttaattttt
    28021 agatggtcac caggaacacc tataaccctt tttacaagct cttttttcct tccattctct
    28081 tgtaataaat cagtcttaaa tatcattata tctccacatt ttggttctga taacttatat
    28141 gtcatcctgt ttgctaccaa ataatcatgt gatttaagtg ttgggtacat tgattctcca
    28201 tttactaatg ttggttttat aaaacgagtt ataataaatg caaaaaaaag tgcagtaata
    28261 attattttta tccactctac aatctcttct ttaatagttt cattcattca caaattctcc
    28321 tttatagtta tattatcttt attgataatg tctcttaaac actatctttt agataagtct
    28381 actaaataag gtgttgtctt ttttatatag acaactattg tgtatggtct ggtgatttca
    28441 gctaatcagc gattgctcca aattttatat gtttagtcaa tagttcctat cttagaaaat
    28501 ggatacaatc tgactagaac ctttcctaaa atggtatgct cgtcgaccaa tccaacctca
    28561 tcgtatctac tatctaaact tttttctcta ttatctccca ttgcaaataa ttttccttct
    28621 ggaactacga tatcaatatc tccatcagta cgattattat gtatatagga agtctcatct
    28681 aacaacttat catttacata taccttggag tcttgtattt ttacatggtc accaggaacc
    28741 ccgataactc tttttactaa atcttttttc tttccatttt cctctgttaa gtcggttttg
    28801 aatactatta tatctttgta ttttggctct cctaccttgt acgcaattct gttaactatc
    28861 aaataatcac gttcaactaa tgtaggatac attgattctc ctttgactat tgttggcact
    28921 ataaaacgag ttattgcaaa tgccaaaaca agagctatga caatcacttt tatccattct
    28981 acaacttctt ttttaactgc ttcacccact aaaatcgtct cctctgtgtt aaatattaag
    29041 tcaatctaca gcaaaataat ctaccataaa cctacaatat aaatattata cttctttttt
    29101 ctaatagttt aaaatacttt tctaagacct ttaggataat gattcttaat tacaccagaa
    29161 gattcttttc cccaacctaa aggtattcct tcaaccgaaa ccaaaaccca tcctctacta
    29221 tcattagaat ttacaacatc cccttttaaa tatttaagaa ttgattcact ctgtacactg
    29281 aagttttcaa cattttttac atcttcaact tttaagtaat gagatagtgc atgagaaggc
    29341 tcaaatctat tcttctttaa aacccctaaa tgcaaaccat atctcaatac ttttagtttt
    29401 ttcgtatctg gatatatttc aggaagtagg tacaaatttt ctcctcttaa ataaaattta
    29461 ttttttttat tactataaaa atcaaccttt aaaaatttct tttcaaactc cctataatcc
    29521 tttatttctt tatctaaatt ttttattttt aattctttga cattacattc ttcttcttct
    29581 aattttttta ttttggcaac aaaatgacct tctcccttta tcttatgagg ccataatctt
    29641 tccatttcaa taagttctgc atgtggatat tcactgataa actcatttat aacatgctca
    29701 ttttcctccc ttgaaaaagt acaagttgag taaattaaaa tgccatcctt ctttaacatt
    29761 ttatatccat ctcttataat ctctttttgg atggattgac attctactac ctttgcataa
    29821 ctccaatctg atatggcaac ctcatccttc ctaaacatac cttgcccaga acaaggtgca
    29881 tctataataa ccttatcaaa atacccatta aacgtctttc ttaagctatt agaatcggtg
    29941 tttgttataa tacaatttct cgctccaaat cgctctaaat tttcccctag agccttaatt
    30001 ctactagcat taatttcatt agaaaccaat aaacctgtat cgtttagctt agatagtata
    30061 tatgtagatt ttccaccagg agcagcacac atatcgagaa ctttttcacc ctctttaata
    30121 tctgcctttg gtattacact cattgcagaa ggctcttgca aatagtatgc tccagcctca
    30181 tgcagtggat ttctaccagg tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc
    30241 catggtattt gattcaaatc aaacaactcc aaatcaaata attttttctt gtcaattttt
    30301 aaagtattta ttctaagccc agtagtctta ctttcctcat aactttttat aaaatcatca
    30361 tattcatctt ttagtatttc ttcaatatct tctaaaaatt cctttggtaa ttctgtcaaa
    30421 tctataatcc cccttcaatc tgctttactt gtgtgtcatt tattatactt taattattta
    30481 tgtctttcct ttaaatttta tttcttcttc tttttcctaa gattaccgaa aaacactttt
    30541 ttagagtgat tcatatattt ctcaagttta tcaagattta tcttccacaa aattattaca
    30601 taaactataa gtaatataat agcccatcta tattctatca tcaatatgaa atcaaaaact
    30661 ccatgtaata aaattggaat taacaccgcc ataaacaaat actcacgtct tttattttta
    30721 tttccctcaa atttatactt agaaatatag tagcccatag taattgcaaa cattacatga
    30781 gctggtatag agataactgc cctattaata ccaacttgca gtgctagatt tctactttca
    30841 gaaaaaatat aaaccatatt ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa
    30901 ataataccat ctaacttttc agtaaagtgt ttttctttaa ttagggcagg aatcagtatc
    30961 agagctttta caccttcttc tacaagtcct gcaactacaa atgatacata tattagttct
    31021 gaataccctt caaatacatt aaacttcatc agtaaatttt ctaaaagtat aattataaca
    31081 cttagaaaag cacctattaa aaaaaattta attagatgga ttaggggttc tctacaatat
    31141 ctatctttag accttatcca aaacatccct atcaatatag gaattattgc taataaaaat
    31201 aaatctagct tcatagatta ttttcctttc aaaatttttg atttatttat gatttatatc
    31261 atctacttat ttttaactta ttcgtgtttt aaatacatac taaaatatac attaaattat
    31321 tttaaattat ttaaatacaa atatactcaa atactcaaaa aaatttaaat ttatatctat
    31381 attatttaac aaatttcaag ctatattcat atcttgaaat ataagggaac taaattcatt
    31441 taaagaaagg gtgatttgat gcaagatggt ttcttaacag taagcataat tgatgctact
    31501 aacaatagac ctattcaaaa tgcagtagta aacatatatt caatgtctaa tggtagccaa
    31561 agctcatcaa ctttatacca aaacctaaga agtaatgaat ctggtcaagt gactggttta
    31621 gtattaccag caccagatgt agattactct ctacaaccat ctgatgttag accttatagc
    31681 cagtatatag tggaagccat agcagatgga tatgagacag ttgtcataga aggaacacag
    31741 ttgttagcta caattgaagc aagacaagga gttcccatgt ctcctagaac aagaagtaag
    31801 cgttctttct caagacaaag tgagttgatt tttgatatag gagaacatac tctatatggt
    31861 acttatcctc ctaagattcc agaaagtaac ttaaaaccat taccacctcc tactggtttc
    31921 gttgtattag ataaccccgt agtgcctgaa tttatagtag ttcatgatgg tttgccagaa
    31981 gattcatcag ctccaaacta ttggattcca tttaaagagt acattaaaaa catagcgtca
    32041 tctgaaatat attcaacttg gcctgaacag acaatctatg caaatgtaat agctataata
    32101 tcatttactt taaatagagt ctttactgaa tggtatagaa ataaaggata taactttaca
    32161 ataacttcaa caacagcata cgaccataag tttataaata atagaaactt atttgaacca
    32221 attaacgtag ttgtagatgc tatttttaat acttttataa aaagacctcc tacatcaaga
    32281 cagccactat tagcacaata ttgtgatgga caaaaatccc aatgtcctga ccaaatgact
    32341 caatggggca gtaaagactt aggtgaccaa ggatatgact atgagagtat actaagatat
    32401 ttctatggag atgagatagt atttgaaaga gcacctatag taagtggtgt accagtgtca
    32461 ttccctggta ctacactaca agttggttct tctgggcagt atgttagaac catacaaaat
    32521 cagttaaatg ctatatctaa ttcttatcca gctgtaccaa aagttataga agatggtatc
    32581 tatggaacag atacagaaaa cgctgttaag atattccaag gaatctttgg tttacctcaa
    32641 agcggagtag tagattttaa aacatggtat gaaatatcca gagtttatgt agctacaact
    32701 agaatagctt ctttaaatcc tttaatttaa agcttgattt agaacataaa ctcaaattat
    32761 tgaataaaat tattaattta aaataaaagt ggctgttatg aactaatata taccataagt
    32821 attacttata agtattgtag tcttaacagc cattttttag ttataacttg tttttttaga
    32881 atcttaaaac tttaaatggg ggattttaag cttcttaaaa acagtgctta gcaacttaga
    32941 tttctttaaa atattatatt tatttttaag tttaataaaa tgctattcta acgaaaaacc
    33001 tactttttat ttaaaatctc acttattatt taaaacacta tccatcactt agaatattac
    33061 ttcttatata aattattatt tattataaat acaaataata ttaatttaaa aagttattcc
    33121 taatttaaat aatactacta tcttcatcag tgaacctaaa ctgcaacttt agacttatta
    33181 gctaaagcca atattagacc taaagctata gatgcaaatg cagaagcaat tactctatac
    33241 tcagcaggga ataaaaatac aattgtatga catggtatcc agaaaaatgg tatagttttt
    33301 aaaacactaa aaccaaggta agcattccag tccacaatat ttaccacatc tttaagtttt
    33361 accttaatcc cctttccaac ctcatatttt ttatctataa aacagtccat aaccttatgg
    33421 aataaaaata cagttggtga cattagtata ttcatagttg cactcttaaa tagagcattt
    33481 gcaaaagcta ttccctcaaa tggaagcata ccatccactt gtgcttgacc tacacctgat
    33541 gagtagattg acattccata tgtggtaact acacctatta ttccccaaac tattgctcta
    33601 taaaatactc ctactggtat tttccaatcc ttatacaaaa ttctaacacc aagtaaatct
    33661 cccattgtag ctaatacagc aaactttaaa aaacctccaa tatatggatg tgcacctgtt
    33721 gcttccataa atacttctct actacttggt attgctaaga cagcaataat acaaataaga
    33781 actccactcc atataaaatc gccttttttc atatttataa atactattta atatataaat
    33841 agttctcctt tctattaaag atttatttaa tttacaatct atctacaatt atttattaat
    33901 cacttattat tagatattca ttttccatat ttaaatttat tctatccctt tatctattta
    33961 ttttcttact actctatcca ccattatcta tatatttatt ttacgattac tctttcaccc
    34021 taacccttta actatctatt ttttcttact attcttatcc acatcatcca tatatttatt
    34081 ttacgattac tctttcactc taacccttta actatctatt ttttctgata ttcttatcca
    34141 ccatcatcca tatatttatt ttacgattac tctttcaccc taacccttta actatctatt
    34201 tttttctggt attcttatct actattatct gtatatttat tttatgatta ctcttttaac
    34261 ctacccccta tactttatag taatatgttt ttaacctttt tcattattcc caatatgctt
    34321 tataactaca aatactctcc ccccttaaaa ataataataa aattaatcaa taaatttata
    34381 atcatattat tactctatgt attaagcaat tactatgcca ataaactttg atttttttaa
    34441 acaaatctta aaaatcctaa aatgctaatt ttctttaact tttaatgaat ctaatagata
    34501 acatttaatt ttcgttatat ttttcagaat atttctatta ttatttttaa gaaaaaaaaa
    34561 taatgttaac atttcttaac atacattaaa aaatgttaac attataaatt gggtttaata
    34621 cttttacttt gactcgattt acttttattc tacttcttac tttattttta acttagactt
    34681 tattttttta ttacttattt gagtcttatt tattttattc tcactccata tgttcaatta
    34741 catttgtcta aattctttga tacttttatt acataatttt aacaagctta agtgttattt
    34801 gggtagtgca gttgataatt atttaaataa tcatcaactg catgtttata tcattacagt
    34861 agcttaatgt atttaatcat acttacttag gattttgaaa tatcttagag attatattta
    34921 acattagtca attgctgcat aattgtaagc aattgaaatt tgagacccat cttcattttc
    34981 tatagtgata ggcttcataa tttttaatcc ttcagttttt atcttttcta attcttgttc
    35041 cattttctta attgtttcct caaggtcttt ttgacttaca tctttgactg cattagggtc
    35101 tttagcatag tcttttaatt cttgaatatg tttagcatac tcttcataag tataatgctc
    35161 aacaccatac ttttccaatt cagtagggtc agttattcct gattttatta gagattcaaa
    35221 tttaggtaaa gcaatattag atgaatcatt tccatttgca aatactgctc cagttcctcc
    35281 tagtaccatt gcagatacca tcattgcact tataagggat ttttttattt tcataaccaa
    35341 tcttcctttc ttagagattt atttgggtag tgtagtttaa aatgactatt gcaatagtta
    35401 tcaactacac tcttagacta tcacagtagt catgtttatg tcaagccttt ttaaaaaact
    35461 tttcaataaa aaatactatc tcaaagcaaa attaattatt ttgagatagt attttaattc
    35521 taaatctttt atattactaa gatttatggt tattctatcc atgttctata ttgttcttta
    35581 ttaactgcta acaaattccc ttttcttaat atgacaaagc gattatcatt atagccactt
    35641 cgctcaattg aaacttctcc tgaatcagta taaagtacat ccttactatt actagattta
    35701 tttacatacg tttcttcact attctcagaa ttagttactt tgctcataga tgattcttca
    35761 tgctctctaa attcaattac agatactttt gccgtactaa aatgagtctc tccatcactt
    35821 ttagctttac taacaagaat atcagattct actttgggag aatataaatc agttgattta
    35881 ctacttattg aaaatccaat tttatcactt tgttcatttt ttgttaaacc aaaagattca
    35941 acctcattaa tctctttatt ttctgttgca aaaacaagac ctgtcccaca aactaataca
    36001 gttgaaacta aaaaagtaac ataagttgat tttttaaatt tcataatatt aactattctc
    36061 ttttctattt ttgtctttcc aaatccacta catattgtca ttgaaaatgg attattctcc
    36121 tctgctagac ttattagtgt ggttgcataa tcagccctat attcttcacc aagtatactc
    36181 ataacctttt catcacaact aaattctatg tctttattta gtaaaaaaaa catcacccaa
    36241 actaagggat taaaccaatg gacaacaaga gctacaagca taaataactt taacagatta
    36301 tcaaagctat taatatgaac catttcatga gttaaaacaa agtctaaagt ttgattatta
    36361 gcataattca tttgctttgg caaaataatt cgtggtttta aaataccaga tgtaagtggt
    36421 gtcctagtaa attcagatat gcaaacaata aatcttctaa aacggctatt cttacacatc
    36481 cattttttaa tgaactcatg attttttata tgaattgaag ttttaaaaag attaattatt
    36541 ttaaaatatc taaagcataa aagtatgcta aacataatag taactataaa agacaaaatg
    36601 cccaacattt ccaacatcat aatattttca atattagaca ttatttcaaa ttcgtcagaa
    36661 ttgtttttac ctgtagagtc actagctttc ttaagagtat aattttctgt tgatgtatta
    36721 cccaattcca catcaaaagt cgattcaatt atactataaa tactaaaacc agaatttaaa
    36781 cttactggta ttagaagttt aaataaaaca atcaaccaca ataatacaaa taccttttta
    36841 ggtaattttt taaataaaca tgctcttata aaaataacta ttaaaagtaa tactccagaa
    36901 gtaaaactca tttctaacaa tttaatgaac ataaatctta cctcacttta atttttcaac
    36961 aatttgttta agttttgtaa tctcatcact tgaaagattt tctttattaa taaaagctga
    37021 aagaaatttt tctgaagacc ctccaaacat tttattaatt aaagtttctg tttcctgttt
    37081 ttgtacttcc tctttgctta ttaaagcttt acaaacaaaa ttcggctcaa ttctctctat
    37141 tgcacctttt gacactaatt tttttataac ggtatacgta gtgtttctat tccatcctgt
    37201 ttcttctttt aaaattttta ctaactctcc agccttgctt tctcctctct cccatagaac
    37261 ttccattact ttaagttctg aatcaaataa ttttatctcc atataaaatc gctcctcaaa
    37321 tatagttatc acaatagtca ttactataat aaaaaactat catagtagtc tatactatgt
    37381 caatcattta ttttttaata ataaaaactg tctttttaaa aaattcttat aaaagacagt
    37441 tttatcaatg ttatacttta atgtttaatt aaagccatat tttatttaaa tatataacta
    37501 tctatcttag tacatagtat aagatttttc tttatctaat agtatatcct ctacatctgg
    37561 gttttctttt actaattgtc tataaaaatt acaattagta acacccacat atggaattag
    37621 ccatagtaga cctattccaa gagtagctat tgaaagtata taccatccta taaagctaaa
    37681 gtttagtaca aataatttaa gtacatttcc tttcatcata tctaaactag cacccattga
    37741 tttaaataca cccatattat ccttgtcttc acaaattaag aaagtagctt gagaaacata
    37801 taatagcaat atataaactg gtaacataag aacaagtatt ataagaacta gagttattcc
    37861 agtagaaata atagtcccta ttccaacatt atcacttgat aaatattcaa ttgctccact
    37921 agtaagtaaa actccaaatg ataaaaacgc tcctataatt gtgattataa aagaataaat
    37981 atacacaata attgattcaa ataaataaaa accaaaactt cttaaataaa ttctcccact
    38041 tacaaagata tctcctatct tgactttttc atctttagta attttcaaga acatacatgt
    38101 aaatcctgct gaaacgcaaa aagctattac agccactaca atagtagtca atggttcaaa
    38161 aggtaatcct acaattgata ttaatactat caatgcaata tatactgcca tagaaattgc
    38221 acatatcccc cagtttccag ataactgagc tttagatact cgttttaatt cttgccttga
    38281 taccataaaa aacacccctt tcattttata aaaaaacttt taaaaatata aataatcttt
    38341 taacgtataa tattaatata tcatattttc gactatataa gatacaatta ttcaccatgt
    38401 tttatcaaga tatgccataa ttgtattact agttttatat agtcaacatt accaaataca
    38461 tcaaataa
//
LOCUS       DAMJYC010000030        38227 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJYC010000030 DAMJYC010000000
VERSION     DAMJYC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.031"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            98..1249
                     /locus_tag="PC472_001695"
     CDS             98..1249
                     /locus_tag="PC472_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2727022.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRSHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1367..2005
                     /locus_tag="PC472_001696"
     CDS             1367..2005
                     /locus_tag="PC472_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2727023.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2006..3028
                     /locus_tag="PC472_001697"
     CDS             2006..3028
                     /locus_tag="PC472_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDF2727024.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3018..3638
                     /locus_tag="PC472_001698"
     CDS             3018..3638
                     /locus_tag="PC472_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727025.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3765..4451)
                     /locus_tag="PC472_001699"
     CDS             complement(3765..4451)
                     /locus_tag="PC472_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2727026.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4465..5010)
                     /locus_tag="PC472_001700"
     CDS             complement(4465..5010)
                     /locus_tag="PC472_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2727027.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5238..6485
                     /locus_tag="PC472_001701"
     CDS             5238..6485
                     /locus_tag="PC472_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2727028.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6572..8566
                     /locus_tag="PC472_001702"
     CDS             6572..8566
                     /locus_tag="PC472_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2727029.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDVVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8566..9888
                     /locus_tag="PC472_001703"
     CDS             8566..9888
                     /locus_tag="PC472_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2727030.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10167..11453
                     /locus_tag="PC472_001704"
     CDS             10167..11453
                     /locus_tag="PC472_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2727031.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11539..12846
                     /gene="grdB"
                     /locus_tag="PC472_001705"
     CDS             11539..12846
                     /gene="grdB"
                     /locus_tag="PC472_001705"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12586..12588,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2727032.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13123..13854
                     /locus_tag="PC472_001706"
     CDS             13123..13854
                     /locus_tag="PC472_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727033.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13856..15214
                     /locus_tag="PC472_001707"
     CDS             13856..15214
                     /locus_tag="PC472_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2727034.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15253..15738
                     /locus_tag="PC472_001708"
     CDS             15253..15738
                     /locus_tag="PC472_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727035.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(16393..16617)
                     /locus_tag="PC472_001709"
     CDS             complement(16393..16617)
                     /locus_tag="PC472_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2727036.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16714..17967)
                     /locus_tag="PC472_001710"
     CDS             complement(16714..17967)
                     /locus_tag="PC472_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2727037.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18227..19216
                     /locus_tag="PC472_001711"
     CDS             18227..19216
                     /locus_tag="PC472_001711"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2727038.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19288..20028
                     /locus_tag="PC472_001712"
     CDS             19288..20028
                     /locus_tag="PC472_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2727039.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20244..21395
                     /locus_tag="PC472_001713"
     CDS             20244..21395
                     /locus_tag="PC472_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2727040.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21420..22175
                     /locus_tag="PC472_001714"
     CDS             21420..22175
                     /locus_tag="PC472_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2727041.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22367..24754
                     /locus_tag="PC472_001715"
     CDS             22367..24754
                     /locus_tag="PC472_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2727042.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVDDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(24985..25218)
                     /locus_tag="PC472_001716"
     CDS             complement(24985..25218)
                     /locus_tag="PC472_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2727043.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            25507..26097
                     /locus_tag="PC472_001717"
     CDS             25507..26097
                     /locus_tag="PC472_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2727044.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            26211..27056
                     /locus_tag="PC472_001718"
     CDS             26211..27056
                     /locus_tag="PC472_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727045.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            27053..27736
                     /locus_tag="PC472_001719"
     CDS             27053..27736
                     /locus_tag="PC472_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727046.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGNKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            27733..28449
                     /locus_tag="PC472_001720"
     CDS             27733..28449
                     /locus_tag="PC472_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727047.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFVITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            28546..28809
                     /locus_tag="PC472_001721"
     CDS             28546..28809
                     /locus_tag="PC472_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727048.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            28781..29236
                     /gene="def"
                     /locus_tag="PC472_001722"
     CDS             28781..29236
                     /gene="def"
                     /locus_tag="PC472_001722"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2727049.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            29288..29749
                     /locus_tag="PC472_001723"
     CDS             29288..29749
                     /locus_tag="PC472_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2727050.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHVLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            29799..30701
                     /locus_tag="PC472_001724"
     CDS             29799..30701
                     /locus_tag="PC472_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2727051.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            31071..31883
                     /locus_tag="PC472_001725"
     CDS             31071..31883
                     /locus_tag="PC472_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2727052.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            32931..33692
                     /locus_tag="PC472_001726"
     CDS             32931..33692
                     /locus_tag="PC472_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2727053.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            33757..34539
                     /locus_tag="PC472_001727"
     CDS             33757..34539
                     /locus_tag="PC472_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2727054.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            34806..35225
                     /locus_tag="PC472_001728"
     CDS             34806..35225
                     /locus_tag="PC472_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2727055.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNKKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(35328..35594)
                     /locus_tag="PC472_001729"
     CDS             complement(35328..35594)
                     /locus_tag="PC472_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2727056.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            35821..36522
                     /locus_tag="PC472_001730"
     CDS             35821..36522
                     /locus_tag="PC472_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727057.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            36604..37212
                     /locus_tag="PC472_001731"
     CDS             36604..37212
                     /locus_tag="PC472_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2727058.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            37536..38000
                     /locus_tag="PC472_001732"
     CDS             37536..38000
                     /locus_tag="PC472_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2727059.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT        15342 a         3837 c         6384 g        12664 t
ORIGIN      
        1 tgtaaagaaa aaagatataa ttagaactgt agtaataaat gttttattat agataataga
       61 gttttaagta tcttaaataa tatttggagg aatatgaatg gaattaagac caagaagtgc
      121 aaaattagaa gaatttgata aagtaataga attaataaac tatgtattta gaatatcaag
      181 aagtcataaa ccaactatga tggaagaatt tcctctttta ctaagtaaaa ataatataga
      241 aaatatgatt ataatatcag aagatgataa agtggtttct gatgtaaact atctaattca
      301 ggatgtatct attcaaggaa acagattgaa ggttgcagct ataggtggag tatgtactca
      361 tccagattat gaaaagagag gctattcatc taagatattg gataaagtag aagaaaaaat
      421 gttttatgat ggtgtagata ttgtaattat atcaggaaca agaagtctat acagtagaag
      481 aaattgtagt ttggtaaaga gtttttataa atatacaata aaacctgaag atgtaaaaat
      541 agcatatgaa atagttgaat ttgatgaaac taattttgaa aaagataatg atttagataa
      601 aatgattgaa ctatataatc aaaatagtac tagatttata agaacgagag atgagtttca
      661 aaaactattg catgcagcaa ctattgcctg gggaccaatt ggttacaaga aggtatttat
      721 aaaggaaaat aataatataa taggatattt gataataaga acaataaaaa aagaagattc
      781 tactgtagga gaagttgctg aaatagggtt aaactctgta aatgttgaaa atatattgaa
      841 atatgttgca aataaatttg gattagaata tttaaattat aaagtacatg tgaaaaatct
      901 taaagaccag ttgaaatgta atgggacaaa atctttggat tatcaacaag gaactatgaa
      961 aataataaat tttactaagt tgtgtgatag tttaagaagt tattttagtc aatatgtaga
     1021 ttttgaatta ctaaaataca tggaatttaa acaagttgaa aataaatata ttattaaata
     1081 caaagaagaa gaacttgtaa tagaaaattt ggataagctt aataaactgt tttttgaaaa
     1141 aaatgaggaa cagtacaatg aatttaaaca tctaaaaaat atatatgaat ttgctacaaa
     1201 ggcatttcct gtagattttc catggactgc aaatttaaat tatcaatagt tttagatgat
     1261 attaaataaa attgattgat acctagagtt agtccttgtt aaataagaga aattattgga
     1321 tattgaaaaa gcaattttgt aagtaattag aaaggatgag tattatatgg acatacttga
     1381 tgatatagca gaatgtatat taaatatggg tgtagataat atagagaatt tagtaaaaat
     1441 tgctatagat aaaaatattg atgtggaaga tatttatgaa tatggtttaa acaaaggtat
     1501 gataggagct ttagataagt ttgagaataa ggaatactat ctttcagaag ttatagtatg
     1561 cacagatgca ttaaataaag gtattaatct tttgaaggga actggaaaag taaagaaaaa
     1621 atcaaaggga gtaattctga tgtctgtagt agaaggagat actcatgaga taggaaaaaa
     1681 tattgtaaaa gtaatggtag aagccactgg atataaggtt attgacttag gtgtaaatag
     1741 aaaaagtgaa gatataatag aagaagctat aaaaaacaat gtagatataa taggcttatc
     1801 ttcaatgatg acaactacaa tggaaaatat gaaatcggtt atagacgaac ttaatatgat
     1861 tgagatagat aaaagaccaa aagtaataat tggtggaggt cctgtaagta tggagtttgc
     1921 agaagaaata ggagcagatg gatacagttc aaatgctcca aaagcagtta aattaataaa
     1981 taaacttata ggaggtgaag tttaaatgtt ttcagatatg actttacttc agtatataaa
     2041 tagtgaagat agaggttttt ttctgcctga tatgggtaca aatggattgt tcttaataaa
     2101 taaaaaggca tatgaagtat atgatagtgt agatttgcaa ttagaattag caaagactat
     2161 ggacaagtat tttgaatcag attttatata ctcattctgt gatgggatta cattttgtga
     2221 aactctgggt ttagagacat taagaccaga ttatgatttt ccaagtgtat tgacacatac
     2281 tataactgac agagataaat taagaaaatt agatgttcca gacccttata cgagtggacg
     2341 tatgccactc aatatagaga gcttatcagt tatatcaaaa agtattaaaa aaccactata
     2401 tgaatcgata caaggtccat ttacacttgc aggtcaatta gctggagcta ctcaactttt
     2461 aagatgtata ataactgata aaaaatttgt agaagaatta ttggaattta caactgaatt
     2521 ggtaagaaga tatgctgtag ctgcaaataa agcaggagca aaatatattt caatagcaga
     2581 gccaacatca gtaaccctaa gtaaagatag atttgatgaa tttatagtaa aaaatttaaa
     2641 taaaatctac aatgaacttg attgttggaa aggtatgcat atatgtggag acaccagaga
     2701 attattagat aatatgctaa gttgtaatat agatgcagta agtttagacc aaattttaga
     2761 ctatgaagaa attgctccta tgattcctag agatatagtt ttgattggta atttagaccc
     2821 aataaaactt cttggacgtt ctaagccaga taagattaga agagagacgc taaaattgtt
     2881 aaagaagatg agaagatatg ataacttttt atgtgatttt ggttgtaact gcttaaatac
     2941 cactccagta gaaaatttac aagcagctat aaaggcagga agaattagtt ataaagaatt
     3001 agataggatt gatattgatg aattataata gtaataaaaa taaatatctt ttaaaggata
     3061 taaatttaga tgatattttt atagataaga atattgtgct aaagtttttg gggtatggaa
     3121 atagaaaagc tccagataca attagtaaaa tcgttgaaag agaaataaga aatataaatg
     3181 atattttaga tatcaagata tatataagtg aagttgatat taataaaaca cctaaagaat
     3241 gtaaaaaagc atttgctgta ctatatacaa taggggataa aattgatttt aagatgaatg
     3301 actatatgga aaattgcaat atgatggcag ggctagcatt ggataaaata ggtatagtat
     3361 gtttagattg tattaatgag aaaataaaaa tgtatttaaa aggaaagtat gatagtctca
     3421 aaatatcgca tgaaatatat ccaggagata aagattttga agtagagaga caaaaggata
     3481 tttataacta tgtaaaaaat aaatataata gtatagaaat agaaatcaat gattatcatc
     3541 aactatcccc cataaaaagt gttgcaatgc ttattttaat gggggatgaa gaaaatttag
     3601 aatcaagatg tagtaaatgt cctagaaaat gcttttagat agttttcaag agtaagattt
     3661 gttttgtgta tgaataattg caataatttt tttggtcatt ttatgtataa actaaaaaaa
     3721 ttattgcaaa gtaatattct atttaatttg aacagactta caaactataa tagtttcttt
     3781 aattcaataa gtgtatttat ttcataagtt ggtatatgat tagtataatt tatactgtta
     3841 ttggaattta accaacaagt atctattcct gaatttattc cacctaagac atcagaggtt
     3901 aagctatccc caatcattaa ggcagaagat ttatcatgac aattaaactt tttgagagcg
     3961 tatttaaata tttcaggatt tggttttgat attttcaact cttcagatac aaccattcca
     4021 tcaatatatc cttttatttt tgaattttca agtcttttct tttgaactgc agaaattcca
     4081 tttgttatta taacaatagt atactttttt ttaagatatt gtaatatatc gattgcacct
     4141 ggaattaaaa atgtacattc acctaaaaaa tttaagtaca ttttgctaag agtttcagaa
     4201 tcaacatcca aagatatttt atttgcaaaa agttcaaatc taagtaattt aagttcattc
     4261 aaagttataa tatttttttc aaggtcgagc caaagcttat cacttatatt tctatatgac
     4321 tctatataat tctcaaaatt aaattctaaa tcaaactcag atagaagttt tttaaaagca
     4381 tgtgattcac ttttcttaaa atcaaataaa gtatcatctg catcaaaaaa tataaattta
     4441 tatgtcttca tggtttttac tcctttacaa gatgactgca aaaattactt tactttcact
     4501 atttgagtca ttttgccatt tatgctttgt tcctaaagga acttttacac tatctccttt
     4561 ggacaaagta aattcatcat catccataaa aagtttaact tctccctcca aaacatatgc
     4621 aatttcatct ccattatgag taatcctatc aacacaagaa gaagtgtttg gagctaaatc
     4681 cataattgca aattctaaat taccactaga gtctggaatt aaaatctcat atataacttc
     4741 tttgcttcca ggtaaaataa ctttttttct attatcatgt cttactatca agtttttttt
     4801 atctacttca gatacaaaaa atgtaaataa aggaacgttt agagaagagg ctatagattt
     4861 taatgaattt acagatggat tagcaagacc tcgttctatt tggctcaata atgatggtgt
     4921 aacatcagct agttttgcta aatctcgaat acttaagttt tgtttttttc gaacttcagc
     4981 tattttttcc cctaaattta aatcattcat ataaatatcc tctcttttta atttaaaaaa
     5041 tattaactta aaataatcaa gtaaaatgat tatgtagtat gaaattatta taacatatag
     5101 aattaggttt tgaaaaaact atgttcaata taattaaata aattaaaata taattgacaa
     5161 agtggataca gaatacttaa aatatattta aataaaatta agcaaaagat agttaattta
     5221 tgataggagg ggatattatg caaataacga cttttggagc tatatttggt ttattaatag
     5281 caattatttt aattataaaa aaatttcaag ctgtatatag tttaatgtta ggagcattta
     5341 ttggaggatt agttggagga gcaaatataa cacaaactgt agattttatg gcaaatggag
     5401 ctatgaatat atcaccatca attcttagag ctttggcatc aggtgtatta gctggaagtt
     5461 taataaaaac aggggcagta gataaaatat cagaacagat tgtaaaaata tttggtgaaa
     5521 agagggcttt attttcgatt gccatttcaa ctatggtact tgctggagta ggtgtaaatt
     5581 tggatgtatc tataataact gtagcaccta taggattata tataggtaga aaattaaatt
     5641 attctaaatt gtcaatacta ttagctatgt tgggaggggg taaagcaggt aatataatat
     5701 caccaaatcc aaatactata gcagtagcag ataatttttc tgtcaatctc tctagtgtta
     5761 tgatggcaaa tataattcca gcaattattg gagttgttat tacagttatt ttagcaagta
     5821 tattaataaa taaaggaaac aaagtacaat catatgaaat tttagagcaa agagaagatt
     5881 taccaagttt atttaaatca ttatgtggtc caattatagc tatatttcta ttgtttttag
     5941 gaaatgtatc tcctatagtt atagacccga tgatagccct tccaatagga ggaatcgtaa
     6001 ctctaattgt cacaggaaac ttgaacaatt caagagaata tttagctttt ggattatcta
     6061 agatgcaagg ggtatgtata ttattacttg gaacagggac tatcgcagga attatacaga
     6121 tgtctgaatt acaacagagt acaataggtg ctttacaatt tttaaatatg ccacaatttt
     6181 tattggcacc agtttcgggt atacttatgt cattagcaac agcatcatca acagctggtg
     6241 ctactatagc atcatctact tttcatgatg ctataataaa tggtgggcta tcaccgattt
     6301 caggtgcatc aatagtaaat gctgggtcta gtgtattcga gcaattacca catggctcac
     6361 tatttcatac tagttcaggt agtataaaca tggatatagg tgaaagattt aagttaattc
     6421 catatgaagc attgatagga atagtaatga ctataatttc aacatctatt caattagttt
     6481 tataataaat ataaatattt aatgaaaaaa gtaggatttt gttattaaat attgaattta
     6541 ttatagtaaa aaagaagatt ggagttttaa agtgaataaa aaaaagattg ttattatagg
     6601 gattatttat tcatttttag tagtattttc acttacaaat atgtatgtaa atatggagta
     6661 taatctaaat gtatttgaat atattaagaa gtcacttcca tttacagaag aagaaaaaaa
     6721 gtggttagaa aaacacaaga atttgattta cagctctgac caaagttcac ctcctttgag
     6781 atataaaggt aaggaagatg gacagtataa gggaattatt gttgatttaa taaattctct
     6841 gtcaattcaa attggtagag atttttattt taaaccaaat aattggtgga aagaatcttt
     6901 tgtaaattca atagatgata gtataaagtt ttttgacttg attccatcaa aagaaagggc
     6961 aaataaattt atatttactg acccaatata cactttgagt gcaaatatat tgaaagataa
     7021 aaagtcccaa gatataaata gttacatgga tttaaagggg aaaacagtag caattccaga
     7081 aggtgattat tctataaatt ttttaaagca aaaaattcaa gatataaaca ttttgttgac
     7141 tccagatata aagactggag tcaaccattt aatgtctgga aaagtagacg tagttgttgg
     7201 tgatgaacca gtattgcgat actatataaa taattatgga ctttcaaata aatacagtgt
     7261 tttaagtaat cctatatata ctaaaaaagc agttcttgct gtacctaaac aatatgaaga
     7321 gctggttagt attttaaata aaggcatttt taaacttcag aaaaatggag tgtataaaga
     7381 tttaaagaaa aaatggtatt ctacgtataa tgaagttgat gatatattgt atgagcgagg
     7441 aattgtgcct agtatttatc tttttatagg tataatttta atatcaattt atgtttttta
     7501 tagctataca tatttgttaa agatagaaat caaaaggaaa acagaacagg taatagaaaa
     7561 taaaaaaact ttagaggcaa cttttaatag tattacagat ataataatgc ttgtagatga
     7621 aaataacaat atagtagaat cgaacaaagt actatatgac tttatgggtg aaatgagtta
     7681 taagatagca gatttgatta gcatgataaa aggtgttatt gaaaatacct tctctgaaaa
     7741 tactaataaa actagtgaaa tagaaataca caataaaatt ttaaagataa acacttttcc
     7801 tgtagagtat aaaaaaaata atactgagta tatagtagtt cttattaaag atataactaa
     7861 tgataaaata gtagaggcga aattacttag agaaaacaaa atgatatcta taggacaatt
     7921 agcttctggg gtagcacatg agataagaaa tcctttgggt ataataagaa ataattgcta
     7981 tctcttaaaa gataacgtta caatggaaga agtaaatgat tgtgttaaat caatagaaag
     8041 taatgtagat agagcaagta atataataac caacttgtta aatttcgcaa gaatatcaga
     8101 tgataattta gaacatataa atataaaaaa ttttattgaa aatatagtga aacttcaata
     8161 taaaatgtta cagttaaaga atgttgaaat aaaaattgac tgtgaacata atttaatatg
     8221 ctatataaat ggagaatcgt taaaacatgt atttataaat ttaatttcta attcaataga
     8281 tgcaatacat caagatggaa aaattattat atactgttat gaaaaaaatc attgtttatt
     8341 tatagatttt aaagataatg gtgaaggaat aaaagaagat gtcctaaagg acatatttaa
     8401 tcccttctat acaacaaaac caattggtga gggaactggt ttaggtttat atattactta
     8461 taatgagatt aaaaagaata atggagatat aagtgttgaa agtaaattgg gggttggaac
     8521 ttgttttcat ataaaaattc cattaaataa agaggtgact atataatgaa aatactatta
     8581 gttgatgatg agctagaata tggagtggta atgaaaaaaa ttctacaaaa gaagggctat
     8641 cttgtagata tagcactaag tggtgaagaa gctataaata taattaaaaa agataaaaat
     8701 tatgatttgg ttttatctga tgttatgatg aaaaatatgg atggagtaca gctattagat
     8761 agaattaaat ctattaatag agatatagag gttatattag ttacaggata tggaagtatt
     8821 gaaaatgcag ttgatgctat gaaaagagga gctttatctt attttataaa aagtaatccc
     8881 attgaaaatt tattagaaga agttgaaaag gtaaaggcaa gtaaaatatc tgtttctgta
     8941 caaaaaaata attttgaatt tactttggaa tctaaaaata gagattttaa taatatcata
     9001 aaaatagcaa aaaaagctgc ttgtaaagat gttaatatat taatcttggg ggaatctggt
     9061 gtaggaaaag atatattagc tagatatata catagcataa gtccaagaaa gaatgagata
     9121 tttgtacctg taaattgttg ttctttttca gaaaatctat tggagtctga gctgtttggt
     9181 catgaaaaag gttcttttac aggagcagta gatagtagaa aaggtagatt tgaactttcg
     9241 aataaaggaa ctctattttt ggatgaaata ggagatatac ctctaaatgt tcaggtaaaa
     9301 cttttgagga cacttgagga caaatctata gagaggatag gaagtaataa atctattaaa
     9361 gttgacttta ggttaatttg tgctatgaat aaagaaccaa aagtggaaat atcaaatgga
     9421 aatataagag aagatttttt ttatagaata agtacaataa caataactat accaccacta
     9481 agaaagagaa gagaagactt aactactctt atagaatttt tcttaaataa gtaccaaatt
     9541 gaacatgata aaaagataca ctcgattgat aaggaagtaa aggatttttt attaaattac
     9601 aattatccag gaaatataag agaattaaaa aatataataa atagacttgt agttttaagt
     9661 gaagaaggta atttatcaaa agacaattta aatttaatat caaataatgt atacatagat
     9721 gataaaataa gtattagacc tttaagagaa ataagaaaag agtttgaatg tgagtatatt
     9781 gaaaaagttt tatctttgtg tggaaataat atttcaaata cagctaaaaa attggagatt
     9841 agtagaagac agttaaccaa taaaatatca gagtataata ttaagtgaga aaaaaacttc
     9901 tcattaagtg agaaaaaaat ttctcaaact gtattaaaag ataaacagaa aaaatctgtt
     9961 tatctttttt tattataatt ttaaaaatta atatttacaa taattattgc taaattcaat
    10021 attggtgtaa attaaaaatg aaaatttcta tgacattaat acatagaata tatattttaa
    10081 ataattggca tagaaaatgc tttatatatt aataaacgta ataaatactt ttaattgcga
    10141 ttaaatatat taagggggat tttcatatga agctagagct agggaatata tttataaaaa
    10201 atgttgaatt tggagaaaag acagaagtaa aagatggagt actatatatt agtagcgaag
    10261 aaatagaaaa gatagtatta gaagatgaaa ggattgtaag tgtaaatgtt gaacttgcaa
    10321 gacctggcga atcaataaga atagctccag ttaaagatgt aatagagcct agagttaaaa
    10381 ttggagatga aagcaaaata tttcctggaa ttataaataa agtgaagact gtaggaagtg
    10441 gaagaaccca tgtactttta ggagcttgtg ttgtaacttg tggaaatata gttggatttc
    10501 aagaaggcgt aattgatatg agtggtccta ctgctaaata tacacctttt tcaaaaacaa
    10561 ataatatatg tattgttata aaagcaaagg atggtataga tactcatgat tatgaagaag
    10621 cagctagaat tgcaggatta aaaataggtg catatgtagg tgaggcagga agagaagttg
    10681 agccagatga agtcattgta tatgagacaa agccattatt aaaacaagta aaagaatatc
    10741 cagacctgcc aacagtagca tatgttcaca tgttacaatc acaaggactt ttacatgaca
    10801 cttattacta tggggtggat gctaaacaga tagtgcctac atttatgtat ccaactgaaa
    10861 taatggatgg agctataata tctggaaact gtgtagcacc atgtgataag gttacaacat
    10921 tccatcattt aaataatcca gttatacatg acttatataa aagacatgga aaagatttaa
    10981 actttatagg agttattttg actaatgaga acgtattctt agtcgataaa gagagaagct
    11041 ctgatatggt agctaaatta atagaatttt taggagttga tggagttcta gtgacagaag
    11101 aaggttatgg gaatccagat actgacctaa tgatgaattg tagaaaatgt agtgaagttg
    11161 gagcaaatgt agttttgata acggatgaat ttcctggaaa agatggcaag agtcaatcta
    11221 tagcagacgc tactaaggaa gcagatgcag ttgtttcttg tggtcaagga aatctagtgg
    11281 ttcatttccc agcaatggaa aaaattatag gaactttaga ttatgttgaa atgatgatag
    11341 gtgggtataa aggatgctta aatgaagacg gaagcatgga tgcagagcta caaattataa
    11401 tagcatcaac tatagcaaat ggctataatc atttgacagc aagatattat taaataagta
    11461 tttgaatata aagttctgtt gatgtaatca ttggtctaac tgatgtaata actataaagt
    11521 gatttggagg attaagagat gagtaaaatc agagtggttc attatataaa caatttcttt
    11581 gctggtatag gtggagagga aaaagcagat attccgccag aaaaaagggc tggtgtagta
    11641 ggacctggaa tagcatttca gagtcaattt aatgatgatg cagagataat ctcaactgta
    11701 atttgcggag atacgtattt tggtgaaaat attgagagtg caactcaaga acttttaaaa
    11761 atgataaaag aagaaagccc tgatttattt attgcaggcc cagcatttaa tgcaggtaga
    11821 tatggagtag cttgtgggac tatttgtaag gcagttgaag aagagttaaa tattcctgta
    11881 attacaggca tgtataaaga aaatccaggt gtagatatgt ttaaattaga tttgcatata
    11941 gtgtctacag gaaattcagc agcaagtctt agaaaggtag ttccaataat gacgaaacta
    12001 ggtcttaaat tggttagagg tgaggaaata ggacctcctg aagaggaagg atatataatg
    12061 agaggaataa gaaaaaattt cttccatgaa cttagagggt ctgaaagagc aattgatatg
    12121 ttggttaaaa agatgaaagg ggaaagcttt gaaactgagt atccaatgcc tgaatttgat
    12181 agagttacac ctatgaaacc tgttaaagat ttatctaaga taaaacttgc cctagtaact
    12241 tctggaggga tagttccaat tgataatcca gacaaaattg aatcttcaag tgctactaaa
    12301 tatggaattt acgatttaac agatatggat tctatgtcaa ataaagaatt tacaactata
    12361 catggggggt atgacagagc atatgtcctt gaaaatccaa atttagttgt accactagat
    12421 gtagttagag agcttgaaaa agaaggtgta attggagaat tagtagatta ttttataact
    12481 actacaggaa caggaacgag tgttggcaat tctaagagat ttggagaaga gttttctaag
    12541 aaattgttag aagatgatgt agatgctgta attttaactt ctacatgagg tacttgtact
    12601 cgttgtggag caacgatggt aaaagagatt gagagagctg gtatagcagt tgttcatata
    12661 tgtacagtag taccaatatc acttacaata ggagctaata gaattgttcc agcagtaggg
    12721 ataccatatc cacttggaaa tcctatgtta ggagaagagg aaagtaaaaa aattagaaag
    12781 aatatagttt taaaagcttt aaatgcacta acagaagata tagaagaaca aacagtattt
    12841 gaataataaa aatacaacac aacactgaaa taaaaaaaga tatcaaaaaa taatttttta
    12901 aaattatttt acgatatctt tttttattag gtaaattcga aatttgcatt tttttaaatt
    12961 aaatttataa aagtgataaa ttagtttatt ataatattga tacacaaatt caaattagat
    13021 atactatgaa aagcgagatt attacatttt ggaacaaata ttcaacatat tagatcatat
    13081 taattgaaca gaaaaaatta actttatata ataattagaa taatgataag aaagaaggtg
    13141 tatatattga tttcaattgc catttgtgat gatgaatcct atatgctaaa tcatttaaag
    13201 gaaaatatag aattatacat gaagcagaag aatttattat ataatataga gctttttaat
    13261 aaagtagaag atttactatg caatcatgaa aagtttaata ttgtattttt agatatacag
    13321 atggaagaga ttaacggaat gcaggcagct agaaaattaa ggtcatatgg taatgagtgc
    13381 tttataattt tcgttacagt tttaaatgaa tttgtatttg atgcttttga agtagatgct
    13441 ataaattatc tgataaaacc tctatctaat gagaaactat ataaaacttt ggataaaata
    13501 gttacaaaac ttgatgataa tgaaagtaat tatatcacaa tacaaaaagg ttattcatat
    13561 tggaaattaa aacttgctga tattttatat tgtgaagtta taaaaagaaa gatttatgtt
    13621 catttaaaga aagagactgt agattattat gagactattg aaaatttaga gagaagttta
    13681 ccaacaaatt tttttaggtg tcatcgtagt tatataatca atttacaata tgtatatgga
    13741 tatgaaaatg gtaatgcagt tatggaaagt ggagatagaa tacctgtttc tagactacgt
    13801 caacaaatat tttcaaaagt tatgcttgag ttcatgaaaa ataggaggat ataagatgat
    13861 taatttattt tcttccgatt tatttttaaa gtacagtttt ttcttctctt ttattgtata
    13921 tagaatttta atggtaatag tgcaatacta ttttctaatc aattttacaa attttaattc
    13981 aaaattttgg tgttttattt tatattcgat aatttcaatt tttttaatca ttataaatac
    14041 atattttaat ttatataatt tattttcttc acagattcta ttttttctta tgactttttt
    14101 atttgtaaag tacataataa aacaatctac tgttttatct atagtatcaa ctttgttagt
    14161 atatttaatt gaacaattag tatatggatt tacagctcca ttaaattact tgatatttac
    14221 tagtgatata aatacagata tttataaatt tgatataaat atgggactat cttctttaat
    14281 tactattata atagctggat ttatatattg gtatagttct aaaaagttta atattaaggt
    14341 catgaacttt gataaatata tagcaatttt gatgattcca ttgttgctta taatattatt
    14401 tatgcaatca tttgaatata gttcaaatat aaatatagat acaagtcttg ggattgtaaa
    14461 gctgctatta aatacttcta taactgaaga aattcaagct tttctttttt ctataattgg
    14521 aactatatgt gtattttttt ctctatttac ctttaagaaa ctaattcaag ctctcgaaga
    14581 tgacaaggag agggcaatta tgaatcaaca gattcatgct caaaaaaact atattgaaga
    14641 agcgaaatca cgattatcac aaactatatc ctttagacat gattttaata atcatttagc
    14701 tattgtcaat ggattgttga aaaaagacca aattttaaaa gcacaagatt atttaaataa
    14761 gcttgaaaaa gtaacaagtg acctatcatt ttcttgtaat acacagaata ttgttattga
    14821 tgctcttttc aacaataaat taagtatagc aaaacaatta ggcatagagg tagattgtga
    14881 agtaattata ccccaaagca ctaacatgaa cgattttgac ctatgtatag tgtttgctaa
    14941 tgctattgac aatgcaatta aagcttgtag agttgtagat aaacaaaata ggtatttaaa
    15001 gctttccact aagttagagg gtggattttt tatgatagaa ataaaaaata gctataatcc
    15061 aataaataat tatacaagtg gctcaggtat aggtctttta aatataaaag aaattgctaa
    15121 aaaatatcaa ggggctataa gtatagataa gacatcagac tattttcaaa taaatgtatt
    15181 gctagttatt tcattacatt gcgctagtat ttgagaacaa tttcattgaa aatagtattt
    15241 gtaagctata ttatgtactt gaatagagaa agtttgatat atagaaatga ggaggttact
    15301 aaaatgaaag ttggaaatat aaatggtcaa gcaccaatta caaatggttt agatagttgt
    15361 caaagtgagg tacaaggctt agaacaaaag aaaaaagcat tacaggatga aatagagaaa
    15421 gtaagaagcg ataaaagtat tgatggtgaa acggccaaat taaaaataga agctttaaaa
    15481 aaagaaatgg aagagattga aaataaaatt caagagttaa aaggaaataa aggaaaaaaa
    15541 acaaataaag aagaaaataa cgtttcagaa gtaaataata atctattaga gagaaaaaaa
    15601 gaaccttcta ataatgaaaa tgtaaaacaa ttatatgatg tttttgaaag aagcaatgta
    15661 gacgaacaat gggataatac ttatcgtgtt ctccaagatg ataaaaactt aaaagtaaaa
    15721 tttaatagac ctatatagtt tatttataaa aatagttaat attttaatta tcaccacttt
    15781 ttagaagaat acacacttta ttttaaagac ccacatattt atttacagtt agcacattta
    15841 tatgtggttt tataactatt atttacaagt ctatattgat aacttttaca tagtatataa
    15901 tgtttaaata cttattcaaa tttatctata cataaacaaa aaaagagatt ttaaaatctc
    15961 tttttagatt gttgacttat ggctttttga tttaataaaa tcaagaagcc gtttgaattt
    16021 tatatgttgg ctgataccct tctgatattg aaacaccatt tttgtttcca aaatcgttta
    16081 aaattaagaa cttattatga atgactattt cattatgaac aataagcaaa taacatgaat
    16141 cttcaatcaa tgttttaaca gtagcattaa atgcttgctt atatagttct atcgtttgtt
    16201 tgaactgccc attaaagtaa atatgagtca ataacataac tatatctaca caaattaata
    16261 taaatttttt atactgtaca ttatctaact gttgattatc tgtattacaa catgattgaa
    16321 tttaaaaagc catcaacaca tagaatgttg atggcctgaa aatagatttt aaaatctctt
    16381 ttttattata tattaattat tttttaaatt tatatttttt gcatcagatt ttctaatagc
    16441 taagtcaaac cctctaaatc taatcactat agggtcatta agaggagcta tctttttcac
    16501 ttcaacttca gtgccatcta ttagtcctaa ggctagaagt ctcttcataa gtttttcatt
    16561 tccagctata ttgtctataa ttcccttttg tccaagtttt aagttgtaaa cagtcattat
    16621 taacacctcc taatgaaaat cattatcaaa ctttatattt ataatatata acaaaaagag
    16681 caaattgtca atatataaca aaaaataaac atactaagtt agtatgttta taaaaaatag
    16741 tatacatggg atactaatag catctagcaa aaaaccactt actaatggta caactaaaaa
    16801 ggcttttgga gaatcaccat atttttgtgt caaagaactc atatttgcca gagcgttcgg
    16861 agtagctcct attccatgtc caatcaaacc acttattatg atggcagaat caaaatcttt
    16921 tccaagtact ctaaagcaaa taaatattga aaataggatt ataaagatta cttgaaaagt
    16981 tactattaag agaataggag gtaataatgt agataattca aataaatcta tattcataag
    17041 tgataaagtt aaaaaaattc ccaatgaaag ttcttttaaa aaatctataa gtttaaaatt
    17101 taattctagt atatttatct tatcatttaa atttctaaaa ataacagccg aaaacataca
    17161 agttacaaca gagggtaata gtatattaaa cgttatatag aagcatctac ttataatttc
    17221 tcctgtattt atacaaataa atatgagaag aacctgttcc aaaaatacag atggtgttat
    17281 tgtttcggag aagttattat tttttatcct attcttattt ctaaaaaatt tacttccaaa
    17341 tctctttaga tttctattat atctaggaat cgacaagtct atatttgatg atggttttaa
    17401 tttatattta tttattagaa atttagctac aggacatccc aatataccac ccataataag
    17461 tcctattgtt gcagcagata atccaacccc aactgctcct tctataccga gattttctat
    17521 tgtaagtcca aatgcagcag aataaccatg accaccttcc atagatacat ttccacacat
    17581 aagccctaaa agaggttcaa gttttataat ctttgatatt gtaatagcta atatattttg
    17641 acaaaatgcc aatactccac acaaaatcca atatctaaat aatacactac cacctttctt
    17701 tataagattt atgcttattt caagtcctat agttgtaaaa aagaaggtta tgaaatttgg
    17761 cattaaattt ccacttatag atatatctgc tatattaaaa tatttaaaaa ataaatttag
    17821 aaaacaaaat aatagacctc ctattactgg tgcaggtata cagaaattat ttaaaaagtt
    17881 aactttattc ttcaataaat taccaattaa aaagaagaat atagatagtg tcaaggtttg
    17941 cactatatca aaacttaagg taatcaaaat aagtcctcct ttgtaattca aagttttaat
    18001 tatataactt aaattcttat gtattgaaat tttattatca aatgtatatt aataaatgta
    18061 tattaaaaag aatcagtaga tataattaga tagtcataaa ttaaattaaa atattttaat
    18121 gagatatgac ttttttagaa ttaaaaaccg tagtatattg atataataga aataagactt
    18181 aaaaatctgt tgatagtatt atttataagt acagtaggag gataaaatgt ttgaaaattt
    18241 acctaagata gatttacatt gccacttaga tggaagtgtt agagtagaaa ccatgcttga
    18301 tatagctatt aaagagaaaa tagatttacc ttcaaataat atggatgaaa taaaaaaatt
    18361 agcaaaggtt tcttttaatt gtacttcact agatgaatat ttagaaaaat ttgatttacc
    18421 cttaaaagta atgcaatcta aggaaaattt aaaaagaatt acttttgaac ttttggaaga
    18481 tgcaagtaga gaaaatgtaa aatatattga aattagattt gcaccattac ttcacaccca
    18541 aaaaggtatg agtgttaaaa atataattga gggtataatt gaaggaatta gagaagctga
    18601 aagtatttat gatataaaag gcaatttaat attaggttgt atgagaacaa tgacctcaaa
    18661 agaggcatta ttagtaattg aagaagggaa atcatttgta aataaaggtg ttgtagcagt
    18721 agacttgtgt ggtccagaga aagagggatt ttgtaaggaa tataaagatg tttttaaatt
    18781 agcaagagaa tatggatata aagttacaat acatgcagga gaagctgcaa gtggagaaaa
    18841 tgtattggat gctataaata ttttaaaggc ggataggatt ggtcatgggg taaaaattaa
    18901 agaccataaa aaggcgtata atttagttaa agataaaaaa atattgcttg aattatgtcc
    18961 aactagtaat gtacaaacta agacagtaga ttcttatgaa gcacatccat tttatacttt
    19021 ttataaagat aatttacatg taagtataaa tactgataat agaactgtat cagatataaa
    19081 tttaagttca gaactaaatg ttatatttga tacttttaaa ctaggattag aagactataa
    19141 aataatttat agaaatgcag tagaagctag ttttgcagat aaagaaacta aagagtattt
    19201 gaatagttta atttaataga taaaaatatt taaactttaa attaagctat taaattgata
    19261 aaaatacatt gtattaggag aaaaaatgtg aaagattatg aagatgttgt ttatattaaa
    19321 ctagtcaaaa aagaacttga agaatggaag aaaaatatat taaagaaacc ttctatattt
    19381 gacaaagcat caaagggtat acagaataaa tttaatggag tattgccaga taaatatcat
    19441 gaaataatta cttcttctat aaaagtaatg acaaaaaccg tactgtttgg atataaatat
    19501 gctacaaagc caccacttag aaatatgtca ttagaagaaa gagaaaaatt agttttagaa
    19561 aaagctaaaa cttacaagac tacagcaatg attgaagggg caggaacagg ggcaggagga
    19621 atcctcttag gtttatctga ttttcctttg cttttaggta ttaaagtgaa atttttatat
    19681 gatgtagctt caatatatgg atatgatgtt aaagattata gagaaaggat ttatatatta
    19741 aatatattac agttggcttt ttctagtcaa cagcatataa acaaaacttt taaattgatg
    19801 aataactggg atgaatatgt aaatagtcta ccagaagaca tcaatgattt tgattggaga
    19861 acatttcaac aagagtatag ggattattta gatttagcta agtttttaca actaatgcct
    19921 ggaataggag catttgtagg attttatgtg aatggaaaat taattgataa attatgtgaa
    19981 actgctgtgt ctgcttatag attgaggatt cgagagctta agttataaga aaaattataa
    20041 cataaaaaat tagttataca atatatctat atgtatctaa ttttgcattg ataaaaacaa
    20101 ttatggataa aaggtaaagc atgatagaat ctaatgtaga ggtttgtatt ttaatggaaa
    20161 aatttagttt ttattgctta agatacaaat tgaattacga agagattgaa ttatgaagag
    20221 aaataattgg gaggaatatt ttcatgacaa atttgccaaa acaatttgat agcttcaatg
    20281 aagctagaca aaaaggattt ataaatgcaa aggaacttaa ggaaagtggg aaaaagatgg
    20341 ttggagtttt ctgtacattt actccagttg aaattccaat ggcagctgga gcaactgtag
    20401 taggtgtatg cggcgttagt gaagagccaa tacctgatgc agaaagggtg cttccaagaa
    20461 atctttgtcc tttaattaaa tcaagctatg gacatgcaat aacagatacc tgtccttatt
    20521 tctatttttc tgatttgttg ataggagaaa ccacttgtga tggtaagaaa aaaatgtatg
    20581 aagaacttgc aaaagttaag ccaacatatg taatgcattt gccaaatact tcaaaaggag
    20641 aatttgccta taaactttgg aaagatgaaa tgataagatt aaaagaagag gttgagaaat
    20701 ctttaggtgt aactataact gaagaagata taagaactgc tataaaagat aaaaatgaag
    20761 aaagagaact tttaaaagag ttttatgcac ttggtaaatt acaaccatct gcacttacag
    20821 gccttgagtt acataatgtt ttatatcaag caggttttaa atttgaccgt gcagaactta
    20881 aacattcttt aagaaaagtt atagatgata tgaaagaaag atatgaaaaa ggtgaatgtc
    20941 ctgttcaaaa agataagcca agaatattga taacaggttc tcctattggt ggaataagcg
    21001 aaaaaatagt taaaacttta gaagatgcag gggcatcagt cgttgcatat gaactttgtg
    21061 gagctattag atcaaacgat ttattggttg atgaagaaat agaagatgta tatgatgctt
    21121 taacacaaaa atatataaat ataggttgtt cttgtatgat gaataatgac aatcgtattg
    21181 aattattaga tagaataata gatgaatata atgtagatgc tgtaatagat gtagttttac
    21241 aagcatgtca tacatttaat atagaaagtt atagaataag agaatttgtt acaaaagaaa
    21301 aaaataagcc atttatgtct cttgaaactg attattcaaa atcagatact gaacaattaa
    21361 gaactagatt tgaagcgttt gtagagatgt tgtaggaata aataaaagta ggaggaacta
    21421 tgtttagtat aggagttgac tcagggtcag tggcaacaaa gggtgtgcta tttgatggtg
    21481 aaaaaattat taagaaaata attataccaa ctggatggag ccctaaaagt acatctaaac
    21541 aggtctatga gttgctaagt agtgaaattg acaaaaaaga tataaaaaag gtggtaggta
    21601 caggttatgg aaggggagta atggattttg ctgataagaa agtaacagaa ataacttgtc
    21661 atactagagg tatttacttt ttaaacaaga acataaggac tattttagat gtaggtggtc
    21721 aagatagtaa agttataaat ttagatagag atggaaatgt atttaacttt ataatgaatg
    21781 ataagtgtgc tgcaggaact ggtcgatttt tagagattac ttctaacctt ttaggaagcg
    21841 atattgaaag tatagataca ttagctaaag gatatgaacc agttaatata tctagtatgt
    21901 gtacagtttt tgctgagtct gaaattgtaa gtcttctagc tcaaaacatt tctactggag
    21961 aggtagcagc agggatactt aaatctattg caaataaatc cacctcaatg ttagcaagag
    22021 gtgaggtaat agatgaagtt gcatttactg gagggcttgc aaaaagtaaa gaactagtaa
    22081 aaatgataga agaaatactg ggtaaaaaaa tttttattgc agaagatact caaataatag
    22141 gagctttagg agctgctgta ataggattta gataaaaaca tataaaaaga aaatgagaag
    22201 ctgaatttat tataaataaa ttcagttttt tattttattt tatttattta taaaggaaaa
    22261 ctttggtata attgatttaa atgattgtat atttgtaaat acttgatatt tagtcatata
    22321 taacattata attttaaaaa ttagctcaaa ataaggggga gaaagcgtga aaaaatttac
    22381 ttcaaaaaaa gtaacaaggt ttgttgcgct gtttttaata tctattttag taatgtcaac
    22441 aattcctgta tcagctgaca atagcaatac attgaatcag ataaaaaaag cagaatacag
    22501 tgagacgtat aaacaatatc taaatgatgc taaaaatgga cgtacagaaa aatataatgg
    22561 gattatacca aatccatatc agttggaagg tactcaaatc caaagtaaag gaagaacagc
    22621 tccaacatct tatgacccta gaaaattagg tcttatgaca tcaataaaaa atcaagagga
    22681 tttaggaatc tgttgggatt ttgctgcaat ggcaacctta gaaagtttct taaagttaaa
    22741 taattatgga gactatgatt tatcagaaga acacttaaga tggtgggctt cagatggtga
    22801 atatggatgg tctgtagatg atatgaatgg tgctttaaat tatgaagcta tgggatattt
    22861 aacatcttgg tcaggtccta aattagaaaa agatatccca tacaatggta gagtatctaa
    22921 agcacaagga gcaaaaaaac caactaatat gaatacagca cctactgttt ttaatgtgac
    22981 agatgctgtg tgtgtaagta atgacataaa ttctacaaaa aatgctatat tacagtatgg
    23041 agcagtaaca tctggttatt atgaagatat taaatatcaa agtgatgacc aaaattcata
    23101 ttattgtcca acaaaaagtt ttaatacaaa tcatgctatt tcaattgtag gttgggatga
    23161 taactattct aaagataatt ttaatgcaaa tattagacca tcaaaaaatg gtgcttggtt
    23221 aataaaaaat agctggggag attataattc tgagggtgga tatttctgga tatcttatga
    23281 agacaagaca cttatgtctg atatagataa tttttcaatc aaaggtggta aaaaaccaaa
    23341 tgatgatgag aagatgtatc aacatgacta tgcaagtatt gtacctttaa tttcaaaaaa
    23401 gataacagct gcaaatgtat ttgatttcaa tagaggcgat gaaactctaa aatctgtaat
    23461 gttcttaact gaaagtatag gagctaagta tgaagtatat tatgctccag tagtaaatgg
    23521 tatacctcaa gaaaacaata tgaaaaaatt aaaagaaggt actgcccaat attcaggata
    23581 cataacagtt ccaattgatt cttttgatat accagaagga aagggtgcaa ttgtagtcag
    23641 catagagggg aaaaatggag agtcaactat aggttctgag tcaaatgtac caggatatga
    23701 tacatttaaa gcaaaagcta atttaggtga aagttatatc atagataata ctggaaaatt
    23761 ctttgatata aatagagata gtaacttcta tccgtgtaat tttactataa aagctgttac
    23821 agaaaagtct tctggtgaga gtatcccaaa tgagtcttta attggtagtg atagatatga
    23881 aactgctatc aaagttagtc aaaatggttt caattcttca gaaaatgtag ttttagtaaa
    23941 tggcagttca atagtagatg cattggcagc aactcctttt acatctgcta taaattcacc
    24001 aatattattg acacaaaaag aagctttaaa ttcaaaaact aaagctgaga tacagagatt
    24061 aggtgctaag aaagtatacc taataggcgg agaaaattct ataagtaaag aaattgaaca
    24121 acaattaaaa agcttaaata tatcgataga gagaatatca ggaagtgata gatacaagac
    24181 aagtttatta ttagctcaaa agttaaatgg tataaaaaat gtatctcaaa tagcagttgt
    24241 aaatggagtg aaaggattag ctgatgcaat tagtgtaggc gctgcagcag cagaaaataa
    24301 cataccaatc atacttgcaa atgaaaagag cgaattgcaa ggagctgatg aatttctaaa
    24361 ttcattaaat atagagaaat cttatattat tggaggaaca gcatccctat caaacaatct
    24421 agaaagtaag ttaaaaaatc caactagatt atctggtagt agtagagacg aaactaattc
    24481 aaagataata gataattttt ataaaaaaga tactttaaaa aatgcatttg tagttaaaaa
    24541 tggaatcaag aatcaaaatg atttaataga tggattggca gttggtcctt taggagcaaa
    24601 aactgggtca ccagtaattt tagttggtga taaattagct gatagccaaa aagaagttct
    24661 taaaaataag actttagaaa aagtaactca agttggtgga ggagcaaata aaaatgcatt
    24721 taaagagtta gtaaaattaa aaagtgctaa atagagtact taagtattaa catatattag
    24781 tgtgtattag taacttatct taataaaagc tgaaaataat tacttcaaat tattgataaa
    24841 taagataatc aatgattgta aagttttgtt ttcagcttta aatttatgta gtataagttt
    24901 aaaaacataa aaaagggtta ctttatatag aaatatagaa aatcttacat tttatatata
    24961 aataacccat atttaattaa ctttttatag tgttctaata gatattattg aacttaatgg
    25021 taaatataat gtgtaagttt catttaaagt tggattagat tcatctatat atcttgagca
    25081 aagtgtaatt gataagctat ctgcttctat aatttcacca tatagaggac aactgctaaa
    25141 gtaagaatgt acttcaacat atttatttaa gttttcttga acataatcgt aaaaacggtt
    25201 ttttgtagta gtagccataa aatcaactcc tttatttttt attaaagttg aaacttttaa
    25261 aaagataaat atgattactt ctattagaaa ctaagttaaa aaataagcaa atagatatga
    25321 aaattagaat aatatattat ttatatctta attataacac tagataataa aaaatgctat
    25381 ataaaattca gaattttcga aagtttttaa gcttattttt aaatatacta ataatactat
    25441 tgacattaga tagaaaaagt attatattga ctatataaat tattttagtc aaactggagg
    25501 gaaaagatgc ctaaatcata ttctgataaa gaaagagagt atataataaa aagacttaaa
    25561 gaagaagcaa gattgtgtat ggagcaatat ggtattagaa aaactactgt tgatgagctt
    25621 gtaaaacggg taaaaatacc aaaaggaact ttttatctat tttttcaatc aaaagaattg
    25681 ctcttttttg aggtattaag agatatacat gactctatac aaaaagaaat cttatatgag
    25741 ataaatagag tagatgaaag tattacatgt gaacaattga cagatatatt tatgaaattc
    25801 tataggatgg ttgatagtac ttcaatttta aatctcatga taaatggaga atttgagata
    25861 ttggtgagaa agttaccaga ttctataatt gaagaacact ttagacatga tgactttgaa
    25921 attggagaga ttatatcttg cataccaaat gcaaaaaata aagacataga aagctttagt
    25981 ggagcattta gagcagtttt tcttaccatg ctatataagc gcgaggttgg aagtaattgt
    26041 tttgaagatg ctttaagatt aatgataaac ggattagtta tacaacttat ggaatagaat
    26101 ttaaattttg tttgctaata acttttatta aaaattatta acaaacaaaa aaattatatt
    26161 ttattgacca tatagtcaaa atagtcaaaa atttgaaagg gtgatattga atgattaaag
    26221 ttgatgattt atcatttagt tatacagata gagatttttt acaaaatatt aactttgaag
    26281 taggaaaagg ggaaatacta ggatttttag gaccatcagg tgcaggaaaa tctacattac
    26341 aaaagattct aattggaatg ataactaatt atggaggaag tgtcattgta aatggagtag
    26401 agagtaaaag acactcaaat aaattttatg aaaatatagg agttgatttt gaatttccta
    26461 gtttatatga aaagttgact gcaatagaaa atttaaaata ttttgggtct ctatattcta
    26521 aaaaactact atctattgat gagttattaa aatcagtagg actagaaaat gaagcaaata
    26581 aaagggtatc tgagtattca aaaggtatga aatctcgatt aaattttata aaagctttat
    26641 tacataatcc agatatttta tttttagatg aaccaacaag tggtcttgac ccgtcaaata
    26701 gcaaggttat gaaagatata attttatcag aaaaatctaa aggtaaaact attattttaa
    26761 caactcataa catgttagat gcaacagagc tttgtgacag agtagcgttc attgtaaatg
    26821 gtaaaatatc tgctttagat acaccacata atcttattat gtcaaaagga gctattaaag
    26881 ttagatatac ttactttgat aatggagaaa aaacttctga atgttttctg aacaatactg
    26941 ctaatgataa aaatttgaat atgcttattg agaaaaataa acttttatca atacatagta
    27001 gtgaaccaac attaaatgat atttttattg agataacagg gaggaattta caatgaggct
    27061 aaaaaatctt attttaggag atataaagtt tcaatttaag tatggttttt attttttata
    27121 tcttttttta agtattatat atatttgtat tatcaatgta tttccaactt ttatgagaga
    27181 aaaaatagct ataattatga tttattctga cccagcagca atgggattgt tttttatggg
    27241 agctattgta cttcttgaga aaagtcaaag ggttttaaat tccttagctg tatctccagt
    27301 aaaagtatct gagtatattt tatcaaaggt aatttcatta ggagtaatat ctagtattgt
    27361 agctatgttt attgctatta cattaaattt agataatatt ataatatcaa ctataggtac
    27421 ctttttttct tctattatat tttctttgct tggattgata attgcaagta aagcatctag
    27481 tttgaatcag tttattgttt taagtattcc tattgagatt atatgcttta ttccaccaat
    27541 actgaatgta cttcttgaca caaaatcata tgcaaatttg taccctttta atatatgtat
    27601 ttctcttatt agtggaaata aaaattttat aatgattaat attttaattt taatatcaat
    27661 tataataata atttatttta taacttatta ttttatttgt agcagttgga aaaaggtagg
    27721 tggagtaaaa ctatgataaa gattataaat gcgtttaaac aagagtttga acaaattaag
    27781 agagatgcta tgctttttat agtatgtgtt tctccaatat tatgtggagt atttataaaa
    27841 tttggaatac cattaataca gaatatatca cttaacaagt tttattatca attaaattta
    27901 gagccgtatt tcttgatgtt tgatttactt ttagctttta ttacaccttt tatgttcttt
    27961 tttgcttcta caatggtcat attgggagaa attgatgaca gcatttcaag atatttaata
    28021 gtaactccgc ttggtaaaac tggttatctt atttcacgat ttggaatacc aggtatctta
    28081 gcatttgtta ttactatgat tctacttatc tttttttctc taactaaaat ttctttcttg
    28141 ttaaacttat caatttcttt attagtttta ttacaaggaa ttattatatc aatattagtg
    28201 atttctttat catcaaataa actagaagga atggtaatta cgaaattctc aggtgttttt
    28261 atgatgggta ttttagcacc attttttatt ttaaataaag cacaatatat tctgttcttc
    28321 ttaccaactt tttggatatc gaaggcattt aaggaagata attatatcta tatgtttatt
    28381 tcaattgtta tatcattaat atggatttta ttattattta aaaagtttag taaaaaaata
    28441 gttaaataag aaaatttaat atataaatgt aaggttaact tgacagtaat gttgaaaaat
    28501 gttacattta aaatgtaaag ttaaccttac atgaatggag caaatatgaa aaataaaata
    28561 aagatattaa gagaaaaatt aggattgaca caagaacaac taggaagatt agttggaaca
    28621 tctagacagg ctataaatgc tatagaaact ggtaaaaatg aaccttcaat atggttggca
    28681 tatgatattt caagaatttt taatgaacca attgaaagtg tatttttatt tgaagaaagt
    28741 gaaagaaaat caagagcaca gataagtagg ggagaatatt atggctataa gaaagataag
    28801 aacatttgat gatgaaattt taagaaaaaa gagtaagtat gtagaaaacg tagataataa
    28861 gataagagaa atattaaatg acatggcaga gaccatgtac aatactccaa atggaggagg
    28921 attagctgca tgccaagtag gagtattaaa gcgattagtt gttatagatt taggagaagg
    28981 acttattaag cttgttaatc ctgaaattat taaacaagaa ggggaacaaa ttgttgttga
    29041 aggatgttta agttttcctg aagtatgggg taaattaaaa agaccaaaaa aagttacagt
    29101 gcaagctcta aacgaatacg gtgagaaaat agaaattaaa ggttcaggtt ttatggcaaa
    29161 atgtctttgt catgagatag accatttaaa tggtatagtt tttacagata aaattattga
    29221 acatgtaaaa ttgtaagata gattggttat tgaatttata aaatatttta tagtaaggag
    29281 ttaaattatg agattatggc ataaggattt aattgatgta ttacctaaaa atcaattagt
    29341 aagtcaatgg agagagctac ttgctataaa aggttctata gataaaaaag gtactccaaa
    29401 ccatgtatta gtgaataaag tattgaatta tagtatagat gaatttaaat tttatacaaa
    29461 aattgtacat gatgaaatgt taaaaagaaa ttataaacct aatgaactta aatatacaag
    29521 tatattaaaa tggaaaaata gaaattttgc aaatgatata tctaatgaac atagtttaaa
    29581 tttagaaaat ttatatgatg attggcataa taaaatgtat ttaaagcaat gcttatataa
    29641 tttagaagaa aaagcaacat gtggaggaat accaataaat gaatggaata tattactttg
    29701 taaatatagt aaagattatg aattgtggtc tggaaatatc atgttttaat tactatagat
    29761 taacattata gtattattgt atataaagag gtgattattt gtataataaa ataaaatata
    29821 ttccaataaa ttgtgaaata gcttgtaata aattaaaaag gagtatgcca tataaatggg
    29881 atttaaatat ttatcgtgga tgtgagcatg gatgtaaata ttgttatgct atttactctc
    29941 ataagtacat caactccaat aattattttg aagagattta tgtaaaaaca aatatagtag
    30001 aaaaacttga gagacaactt aaaagcagta aatggaagaa agaagtaatc aatataggag
    30061 gagttacaga tagttatcaa ccaatagaag ctgactataa aattatgcca gaaatattaa
    30121 acctgctaat aaaatacaag acaccagcta ttatttctac aaagtcagat ttaattttaa
    30181 gagattacga tttaattgat aagctatcta ggattacata tatcaatgta gcatctacaa
    30241 ttaccacagt aaatgaagat acacaaaggc ttattgagcc aaatggtgtg gattcaatga
    30301 gacgatttaa aatgttaaaa gaatttagaa aaactaacgc atcagttggt ctacatatta
    30361 tgccaattat accatatatt acagatgatt ttgataatat aaattcgcta tttagacatg
    30421 caaaagaatg taatgtacat tatgttttac ctgggacttt atacttaagg ggtataacta
    30481 gaggtgtttt ttttgacttt gttaaaaaag agtttcctga attatttgat aaattatcaa
    30541 tactatatag tgcaggttca cttaataggg agtataaaaa tcaattgtat aaaatggtta
    30601 atgaacttag atataagtat tctctatcaa gcagttactc taaaataatg aaggaaaagt
    30661 taaaaaactc aggagatgta cagatatctt tttttgatta gttttatata aaaaaatttt
    30721 ttttactatg atgattaaaa tttattaaaa ttaaaattta ttgacaaata taaaatttaa
    30781 atatataatt aattcataaa ataaaaatat gaaaggatga tgttgttcgg tgatatttga
    30841 tatcataaaa tcgttgatat aagtattgct ggaaaagagt gaataaaatc aaatttaata
    30901 agcaaacaaa gtaaagctat agttttattg ttgtgcttaa aaatatttgg ttctatttta
    30961 ctcttattta aactcatctt tttgaagtta aaccacagag taaactctgt ggttttttta
    31021 ttttaaaaat aaattttaag gagtggtatg ttttgttaaa caaattatgt atgtatttag
    31081 aaagaccaga gctttataaa caaagtgaaa ttaatttttg ggatgatgag tatatttcaa
    31141 agcaattatt aaaagcacac ttagacacta attttgaagg agcaagtaga aattttaact
    31201 ttatcgagga ttctgtaaat tggatagtta cagttgcaaa tccagctaat tatcctaaat
    31261 tacttgattt aggttgtggt ccaggtttat atgcagagaa atttgcacaa aaaggttata
    31321 aggtaactgg aattgatttc tcaaaacgtt ctattaacta tgcacaaaat agaaataaag
    31381 agactaattt aaacataaat tacttgtttc aaagttattt aaatatgaac tataatgagg
    31441 aatttgattt ggctacttta atttattgtg attatggagc tttgtcaact gaaaatagaa
    31501 ggttattaat ggaaaaaatt tatgatagtt taaaacctgg aggaaaatta atattagatg
    31561 tatttacaat aaataaatac aataattttg aggaaattaa atattgggaa ataaatgaag
    31621 atggaggatt ttggagtaat gaaaaatata tgtgtctaca agataattgt aaatacaatg
    31681 attataatac attagaacaa actcttgtaa taaccgaaaa agatgaaaat gtattttatg
    31741 tttggaatca ctatttttca aaagaaagtt tcttattaga agttgaaaat attggattta
    31801 agtctgttga gttttttagt aatgttaagg gtgaagcata ttctgacgat agtatgacta
    31861 tgggattagt tttacaaaaa taataatttt tttaaaatga ataaataaaa acttgaaacc
    31921 tttaacaata aaataacata ataaaagctt acatattgaa aaatggaaac ttttatgtta
    31981 tttttagtac attacaaatt tgcttatata aaaatcaata cttgaattat tatttttatg
    32041 tgaaatttat ctatagtata tttttataaa caagtaatgt tattaaaatt aagtatatag
    32101 ttaaaagtgt atagcaaaaa ggctgttata ttaatgttag aggttcaggg aaaatatata
    32161 aagcaaagta tttacagata atgtagattg cacagctata agttagataa tagaattatg
    32221 taatcaagaa tttacaacaa agtataagat aaggataatg ctaaattgcc atacaggtgc
    32281 tagatgtgta aatggattta attaatacta ttgataaagt gcaacaagca cataaaccaa
    32341 ttgatgagat tattgaaact ataagagata tagttgacat aattaaaata ttggaacctg
    32401 tttataattt taaatcaaat caataatgat taagattaaa ataatattaa ggcatcaatg
    32461 ttataagagt acaatataag gagtactata tagtactcct tacttataca tattaggcaa
    32521 taggaaattt tttcatagtt aaattttcaa gtatttttta ggaaacttta taattgagaa
    32581 ttaagggtat agtatgtgat gaacttactg aatttataat taggttatat aatggaataa
    32641 tcatcaatag ttttacttta gggaaaatat atgcttaagg aagtatagct tactaatatt
    32701 attaaaatgt atatttaaaa tgataaaaaa agaattagac aagtatttag taaattgact
    32761 atgcaaaatt ctatcaagca ggaaccctat gactttagtc atgggaggtt cagtaggtaa
    32821 cttgttgaat taagtttata ttatttaaat aaactactta atttagataa tataaaaagt
    32881 cagaatttta agaatagagc tatataatct tagctattag gagaattact atgataggaa
    32941 tatattttag tggaacagga aattcaagat actgtgtaga aaaattttta caagaatatg
    33001 atataacagc aaattcttat tctattgaag ataatgaact tttacagcac atcaataatc
    33061 atgagaatat tatttttagt tatccggtgc aatatagtaa tgtgccaaaa atattgaaag
    33121 attttataat aaatcattca actttgtgga atggtaaacg tgtttttatt attgcaacta
    33181 tggctctatt tagtggagat ggctctggtg tgttatctag aatattaaaa aaatacggtg
    33241 ctataactgt tggtggatta catgtgaaga tgccagatag tataggagat gaaaaggtat
    33301 taaaacattc tttagagtat aatagaaagc ttgtaataaa agcagaaaag aaagtaacta
    33361 aagctgctaa aaaactgaaa catgggtcac caccaaaaga agggttaggt tttttgagtc
    33421 atattgtagg gttatttgga caacgattgt attttatcaa caaaacaaat atgtatacag
    33481 ataaactaaa aattaatact gaggaatgta ttgtatgtgg taaatgtgtt aatttatgtc
    33541 caatgaagaa tctagtaatt aaaaattgta tgattgttgc aaatgggcaa tgtacaatgt
    33601 gttatcgttg tgtaaataat tgtgctaaaa aagcaattac attgttaggt aaaaaagttg
    33661 ttgaacagag taatattaaa aaatatatgt aagaaattta aagttttatt gaatgtaaat
    33721 tgatgtatat tttatgttag aaagagaggt taaataatgg aatactacat agttgattca
    33781 tttgcaacta aactatttaa aggaaatcct gctggagttt gtgttcttga cagaagaata
    33841 cctttagagt taatgcagaa aatagcagaa gaaaacaatt taccagaaac agcatttgta
    33901 gttaaatgta aaggaaatta tgaattacga tggtttactc ctaaagcaga aattgattta
    33961 tgtggtcatg caactttagc agcagcttat gtaatatcaa attttattga tgttaatgta
    34021 aaaaagatag atttctttac tcaaagtgga aagcttgaag ttacacgaaa tggaaatcta
    34081 tatgaaatga tatttccaga aataatgcct attgaaatag agttatcacc gcaacaagct
    34141 aatttgatag gctgtgtacc aagtgctgtt tattcttcaa gagatttaat cttattgcta
    34201 aacagtgaac aagaagttat aaattataaa cctaattacg cacaattgcg taaattgaca
    34261 gactggttag gtataataat cacagcacaa ggaagtaata cagattttgt atcaagatat
    34321 ttttgtccag aattagattc agaagaccct gttactggtt catcacattg taatcttatt
    34381 ccatactggt ctgaaaagtt gggaaaacac aaaatggtag ctgctcaact ttctaatcgt
    34441 ggtggtataa ttcagtgtga agtgttgaag gataatactg tgaaaatttc tggtgaagca
    34501 gtcttgttta tgcagggaac aataaagatt gatatttaaa taagtataat aactaattta
    34561 ttatgaaaaa taattaattt tcttagtgtt aacaataata aagtagagtt aaaaaacttg
    34621 atttcactta aatcaatatg atagatgttg atactaagat tgacaagcat gattaaaaaa
    34681 gataatattt aatctataaa gataaaaaca cagtctggtt ggtagtccag acgcagcaat
    34741 agctgtcagt aacctgcctc cttggttgtc cgttctttag aaagtaattt aaggaggaac
    34801 tagcaatgga caaaattagt ggaaaattaa cagtcttatt tgaagaacca ttttgggtag
    34861 gtatatttga aagacaagat ggtaaaaaat atgaagcatg tagggtagtt tttggagcag
    34921 aacctaaaga agtagaagtg tatgaattta tacttgaaag atttttctca ttagattttg
    34981 gtagcattaa attagaaaaa aatgtgacca aagacaacat tggatacaaa agaatgcaga
    35041 gaaaagtaaa aaaggaacaa gaaaaagaaa ctatagggac taaggctcaa aatgctctta
    35101 agcttcaata tgaagaaaga aaacaggata gaaaaaacct agctaaaaat aaaaaagaag
    35161 aagaaaaaga aagactattt aatttgaaac aagaaaagag aaaagctaaa cataagggtc
    35221 attaatttta attaaatatc tttttaataa ttgctagaaa aaggtgtata aaatttttaa
    35281 ttttatacac cttaatttat tgaaaagaat aaggttttat atttcaatta ctttaaaata
    35341 taaaaatata ctaataaaaa ttgtaaaaat agtataaatg gtataccaaa tgtaaattta
    35401 atgtgtttag ttttatgtct aaaagaatac attccaatta tagaacctat actacctcca
    35461 attactgcta tagacataag agtagcttct tttattctcc attcgtttct aattgctctt
    35521 ttcttatcta tgtacattga aaagaaagca atagaattaa tcactataaa atatattaga
    35581 ataaaatttt tcacagtctt gcctccataa aaatatatta tacattataa aacaagatat
    35641 ttgttttgga aagttaaatt tgtaattagt acatataaaa tatttgataa gaataaatat
    35701 aatattaatt ggaaaattta aaaaaattaa acaattagca aatagggggt atatttataa
    35761 gacacaaagt gataaaatct atttagaaaa taatgtaaaa taacaggagg ataaagcaaa
    35821 atgaaattta tagtagaaaa agaagttttt gataaattag aaaatgtatg ctttggtgta
    35881 gtagttgcaa aaggaataga taatactaaa gaaatagaaa gaataagcaa ccttttagat
    35941 ataagtatag atagagtaga agattacttc aaagataaaa aagtaaagga atcagaagaa
    36001 attataccat atagagaggc atttagaagt ctaggcatga acccaaataa atttatgagt
    36061 tcgatagaag caatgacaac aagggttgct aagaataaga aacttcctca tataaatcct
    36121 attgtagatt taggaaactc tatttcttta aaatatttac ttccaatggg tgctcatgat
    36181 atggatttta gaaatgatga tgtatatgtt agattttcta aaaaagggga taaatttgta
    36241 ccatttggag aaactgatgt tgaattaatg gaagaaggag aattaattta ttcagttgga
    36301 gacatggtaa aaactagaag atggatatgg agacaaggag aggaaggaaa aattactaat
    36361 agctcaaaaa atatattttt tcctatagat ggttttactg atgctaattt agataaggta
    36421 atgagtgcaa gagaagaatt agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa
    36481 gtaggttttg tagataaaga taatcctgaa atggaaatat aataagttgt tgaatacttg
    36541 ttaagatatt atatttactt aatgatgtta aaaaggtatt aaaaaagaaa ggtcgtagta
    36601 aatttgaaaa aaactataat aaagaatgta aatccaatga ttgaaaataa gtttatagga
    36661 ctttttgaaa tagagtataa aaacaaatta gatgaagata aggtttggat ggtagcatca
    36721 agaaaaagta gtgagcaatt aaaaaatatt tatttgaaga atgaagaaga tagtgtagat
    36781 gcagttgtaa tagttgggct tcataaaagt agtaaaaaat taattttaat aagacaattt
    36841 agagtaccta ttaatggata tatatacgag ttaccagcag gtcttgttga tgaaggagag
    36901 tctatagata tttctgttga aagagaactt agagaagaaa caggacttac tcttttagaa
    36961 attaaaaaaa ataaaagtat tgataaagtt tatttatctc ctggaatgag tgatgaatct
    37021 gttgcctttg tttattgtat atgtgatggt gatataacag atgaattttt agaaccagat
    37081 gaagagattg aagcgctttt aatttcgcaa gaagatgcaa aagagatttt acaaagtaat
    37141 cataagatag acacaaaagc atttttgatt ttacaaatgt ttgtgagttt aggagctaaa
    37201 ttatttgaat agatatatag aaaaaaggtt atatagaaga gtaaaataat aaagtttatt
    37261 ttacttgtat aaattataat ctcttttttt atacaataaa gtataatttt gtgtattttt
    37321 aaataagtga cttgactctt aaattttcat aatatgttat aaaccaattt aagatactac
    37381 aataattaaa attccaaaat tatttaacat atttgcaatt aaaatgagaa aaggtaaagg
    37441 ctgaatgatt gaaaaatttg atgtaaaata gtagattagt ttaatactat agtttgtagg
    37501 gttaaaaatt agaaattgta agggagataa attctatgaa aaaattagtt attattttaa
    37561 ttagtatcat tagtgtggtt agcttgacag cttgtaaaaa aagcaatgaa agtaaatata
    37621 ataatttaaa caacaaagaa tcaggacaaa caaccattga aatggactta gataaaaatt
    37681 atgatacttc agacccattt gttaatgcac gtttgttttg tgtgtctaat gatatagatg
    37741 ttttagacac agagatttct tttaaaatgg atggagatag tggtattgtg gaaataaagg
    37801 acaataaaac agatgaaact ttatggagta atatatggca tggaagcgtt gataatgata
    37861 cattcgcaat ttctctttct aatatacaaa aagagaaaga gtatgctata tgctttactg
    37921 gaattaaaat aaatcatgca gttgtaaagg ttacttttga aagcaattta gttaaggaga
    37981 aagaaaaacc ctcaaaataa attccagatt gtctataact tgatattcag tacataaaag
    38041 cagttgatat ttatgattga ctgctttttc ttatcaaaaa ggataggttg tgcattttta
    38101 cttgaaatag tttatgcaca tcaaaagttg gtgagcatga tattggaaat ggtaagcatg
    38161 aaatcttgat aagacaagga aataaattta agttttcaat taagtatatt tccataattt
    38221 agttaat
//
LOCUS       DAMJYC010000031        37417 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJYC010000031 DAMJYC010000000
VERSION     DAMJYC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.032"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(17..859)
                     /locus_tag="PC472_001733"
     CDS             complement(17..859)
                     /locus_tag="PC472_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2727060.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1120..3162)
                     /locus_tag="PC472_001734"
     CDS             complement(1120..3162)
                     /locus_tag="PC472_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2727061.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3314..4285)
                     /locus_tag="PC472_001735"
     CDS             complement(3314..4285)
                     /locus_tag="PC472_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2727062.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVLESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISINHFVACHLV"
     gene            complement(4286..5263)
                     /locus_tag="PC472_001736"
     CDS             complement(4286..5263)
                     /locus_tag="PC472_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727063.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVNFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5482..7041
                     /locus_tag="PC472_001737"
     CDS             5482..7041
                     /locus_tag="PC472_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2727064.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEEYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAKIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7147..8106
                     /locus_tag="PC472_001738"
     CDS             7147..8106
                     /locus_tag="PC472_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727065.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8109..8921
                     /locus_tag="PC472_001739"
     CDS             8109..8921
                     /locus_tag="PC472_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727066.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9137..10039)
                     /locus_tag="PC472_001740"
     CDS             complement(9137..10039)
                     /locus_tag="PC472_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2727067.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIVFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10394..11572
                     /locus_tag="PC472_001741"
     CDS             10394..11572
                     /locus_tag="PC472_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2727068.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11581..12237
                     /locus_tag="PC472_001742"
     CDS             11581..12237
                     /locus_tag="PC472_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2727069.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12237..12902
                     /locus_tag="PC472_001743"
     CDS             12237..12902
                     /locus_tag="PC472_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2727070.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            13007..13783
                     /locus_tag="PC472_001744"
     CDS             13007..13783
                     /locus_tag="PC472_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2727071.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13845..15179
                     /locus_tag="PC472_001745"
     CDS             13845..15179
                     /locus_tag="PC472_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2727072.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15675..16520
                     /locus_tag="PC472_001746"
     CDS             15675..16520
                     /locus_tag="PC472_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2727073.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPESNKPEDNNNSDSTNKPDDNNNSGSTSESFSAYQK
                     EVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKF
                     GISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWT
                     QIFVG"
     gene            complement(16912..20451)
                     /gene="nifJ"
                     /locus_tag="PC472_001747"
     CDS             complement(16912..20451)
                     /gene="nifJ"
                     /locus_tag="PC472_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2727074.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(20969..21538)
                     /locus_tag="PC472_001748"
     CDS             complement(20969..21538)
                     /locus_tag="PC472_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2727075.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(21610..22218)
                     /locus_tag="PC472_001749"
     CDS             complement(21610..22218)
                     /locus_tag="PC472_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2727076.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(22226..23989)
                     /locus_tag="PC472_001750"
     CDS             complement(22226..23989)
                     /locus_tag="PC472_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2727077.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(24032..24499)
                     /locus_tag="PC472_001751"
     CDS             complement(24032..24499)
                     /locus_tag="PC472_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727078.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            24683..25144
                     /locus_tag="PC472_001752"
     CDS             24683..25144
                     /locus_tag="PC472_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727079.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            complement(25237..25407)
                     /locus_tag="PC472_001753"
     CDS             complement(25237..25407)
                     /locus_tag="PC472_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727080.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(25596..25817)
                     /locus_tag="PC472_001754"
     CDS             complement(25596..25817)
                     /locus_tag="PC472_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2727081.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(25901..27127)
                     /locus_tag="PC472_001755"
     CDS             complement(25901..27127)
                     /locus_tag="PC472_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2727082.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(27189..28121)
                     /locus_tag="PC472_001756"
     CDS             complement(27189..28121)
                     /locus_tag="PC472_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2727083.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(28210..28737)
                     /gene="hpt"
                     /locus_tag="PC472_001757"
     CDS             complement(28210..28737)
                     /gene="hpt"
                     /locus_tag="PC472_001757"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2727084.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            28966..30042
                     /locus_tag="PC472_001758"
                     /pseudo
     CDS             28966..30042
                     /locus_tag="PC472_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(30128..31336)
                     /locus_tag="PC472_001759"
     CDS             complement(30128..31336)
                     /locus_tag="PC472_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2727085.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(31497..31886)
                     /locus_tag="PC472_001760"
     CDS             complement(31497..31886)
                     /locus_tag="PC472_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2727086.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(31990..32991)
                     /locus_tag="PC472_001761"
     CDS             complement(31990..32991)
                     /locus_tag="PC472_001761"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2727087.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(33050..33305)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(33439..34629)
                     /locus_tag="PC472_001762"
     CDS             complement(33439..34629)
                     /locus_tag="PC472_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2727088.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIANNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(34910..36031)
                     /locus_tag="PC472_001763"
     CDS             complement(34910..36031)
                     /locus_tag="PC472_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2727089.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(36092..36577)
                     /locus_tag="PC472_001764"
     CDS             complement(36092..36577)
                     /locus_tag="PC472_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2727090.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(36579..37247)
                     /locus_tag="PC472_001765"
     CDS             complement(36579..37247)
                     /locus_tag="PC472_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727091.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
BASE COUNT        12679 a         6190 c         4605 g        13943 t
ORIGIN      
        1 attaaactac tctttattat aaattcagta cagatataac tcttttgata tgtaatgtta
       61 aataaagtag ttcatcttga cttacatagt aatcataatt cttttctaca tgagttttta
      121 ttttatatgc acaatcataa tccttttcat actttacttt aattatatct ataatttcat
      181 tatttgtatc attgattttt tcttttttta ctaatcgttt tgcaaagaat tttaaatgag
      241 tcagaagcct atcataattt atatcatctt ctttaaattc aactctataa taatatctta
      301 taatatttag tatgtctttt acaattttag taattaacag tgattccttt gaacttccct
      361 tatagtttga atttactata tgcattgcta taaaaccagc ttcatctact ggaaatttca
      421 catcaaattc tttatttata taatctacag cccatttccc aatctcatat tcttttttat
      481 gtattctttt tatctcatgt agtaaatcat tctttatagt tatgttttca tgatatcttt
      541 taattgcaaa tgcaatatgg tcagtaagtg ctacataaat atattcataa agtttagtat
      601 tcaaattctc ctctgcataa gcaattattt cagtactaac ctcaaaaaat ttctcatcaa
      661 cctggcttgc tagtttacta attttatcta aaaagctttc atcttgtatt acaaaagttc
      721 tttcaatttt acttttatct atttcttgac ctttcttttt ttgaaaccca attccacatc
      781 ctgttaatat taattctttt tttatatttt catctaaaac taatacaaca ttattattat
      841 aaatttgttg aataatcata atcaatatct ctcccaaaat acaatttccc ttattcataa
      901 aaaaatacct aaattaagat acaattcacc tgtatacctt aacctaggta ttgcctgctt
      961 accagtaaca atccagcttg ttaacgctta tatattgtat tcaatttatg gttttattct
     1021 attataaata aatatatatg tcaagtaaat tattgaaaac tatttcctat tttttagtat
     1081 taattttaac atcaaaaatc aaattttatt ccattatatt tatgattcta tcatcatcat
     1141 ttcatcatag agaatatttt ccaataactc tttagccact tctcttgata tttgcttagt
     1201 ttcaactaat tgttgaacac tgtctctttg actttgtatc gccattactc tgtagtattc
     1261 tactcttgcc tcaaacttat catcattttg aatctttcta gtaatccaat tcaaataacg
     1321 attatagaat ctgattgcta ttaaggcctc atgtctgtta tcttcatcag tatttttatt
     1381 tataaatgat atagcagttt tagtagaaag tgcatatgca tggtgaaact ctttgattat
     1441 ttctttattc tcagatacta tttttgtaat tttttctttt acttctttct ttacttttct
     1501 atttataatc cacatgctta tttttaattt tacaatttta aatatagatt tttgaacaga
     1561 ttttcttgat tttgtaatat aaaatctata caatttaaag gctctatcag aaattttacc
     1621 atcttctaat agtttcaaaa cagctaataa tccctgttgg tctgcttctg caatcatcat
     1681 acttataagt ttattatctg gagtatttag tattcctttt tctgagtcta ccaactgttc
     1741 atttaaatca tgtataactg gttgtacttc ttgtgctttt tcattactaa gttttaaaat
     1801 ggcttctttc aaaattagtt tatatgcctc tttatcagat aataaaggtt ctgactcttg
     1861 tacgttattt tttaataatc ttggcagtaa aactgttgcc aatactaaac ttataagaat
     1921 aacttctgat gatataaata aaacagtatc tctcattgga aatacatctt tatcagccaa
     1981 ataaaaaggt atagataagg cagttgctag tgtaaatgta ccatgaacac cacaagtagc
     2041 aactattagt gagtatttac atttagaaat actctctttt acaacttctt catccttaaa
     2101 acctaaaaaa cctacaaaaa aattatttaa aggattcttt ttaggtttta taaaagaatt
     2161 atgccataaa tatacccata taaatcttat aacaaataaa ataagtacaa ttaaagtaga
     2221 aataaaagtc aattccaatg ccatattttc atttttacta ctaagtcctg tatatatact
     2281 tggtaacaaa aaaccaagca gtgtaaagac aaatccattc aatacatact ccaaaacata
     2341 ccaagtattg tctgatatta tccttaattt agtagttgta ttttgtaaat gctctttttc
     2401 aattccatga gctatccctg ccattacaac agctaggatt ccagatactc ctatttcttc
     2461 tgctacaaaa tatacaaaaa gtggagtcat tatttgtata actattacca ttggaatttc
     2521 ttccaaattc cacttgtgta aactaagtct tatttttata attatgtatc ctaaaatact
     2581 tcctactatt attcctccaa aagctgtaat cataaattga atccctgcct cttctattga
     2641 aaaaactcct gtaactgtgg caagtactgc aaccttaaat gctactactc cagcagcatc
     2701 atttaataaa gattcacctt caagtatact catcatattt ttaggaaaat ttaatccttg
     2761 tgtaattgat tttactgcta ctaaatcagt tggagatatt actgctgcca aagcaaatga
     2821 aacagataat ggcatcccag gaataagaaa atgtataaag attccacctg caaacacggt
     2881 tattaaaact aaaccaaatg caagaagcaa aattggtttt ctgagctcta atagctcttt
     2941 tctcgataca ttttgtcctt cactgaatag taatggagct atgatacata ttacaaatat
     3001 ttctggatta aatgaaaatg agaatttaaa tggcaaaata gacaatataa tccctagtac
     3061 aatttgatat attgcaagag gaatattact ccactttttg ctaaatacag ttgaaatcgt
     3121 tgtacaaacc aataatccca acaatgttac taaaattgac ataactacct ctcttttcaa
     3181 taataatatt atagattaaa cttatatatc aactaactta atattaacag catgaaatcc
     3241 catcttaatc aaaaaaattt tcagtttatt ctattattct tattatatac atcaaattta
     3301 atttttgaaa tatctatact agatgacatg caacaaaatg atttattgat atttctttta
     3361 aaactggaat ctcttttttg catatttcct tacaataagg gcatcttgta tgaaacttac
     3421 atccacttgg tggatttgtt ggagatggta tgtctccttt taatataatt ctatttttct
     3481 taattgtagg gtcttgtatt ggtactgcgc tcataagcgc ttttgtatat ggatgttgag
     3541 aatgtaagta aagttctcct ttagttgcaa tttctattat agaacctaga tacataacac
     3601 aaactctatg tgcaatatga tttactacac tcaaatcatg agatataaat aaatatgaaa
     3661 gtttatattt atcctgtaaa tccataagta aatttataat ttgagattga atagaaacat
     3721 ctagtgcaga aacaggttca tcacaaacta taaattctgg atttaatgct aatgctcttg
     3781 ctatacctat tctctgtctt tgacctccag aaaactcacc tggttttcta tttaaatggt
     3841 acttagaaag accacaactt tctaaaacat ttaatatatt atcttttatt tcattctttg
     3901 gcactaaatt atgttctata actgcctcgg atattatttg ttccacattt aatcttggat
     3961 ttaatgaact atatgggtct tgaaatatca tttgcatttt gggtcttaac tttctaagtt
     4021 cttccttctc taacttatat atatctacac cattaaattc aactttacct tcagtttttt
     4081 caattaatct aagaatggtt cttcctgcag ttgattttcc acatcccgat tctccaacta
     4141 tagccaaagt ttctcctttt tttaaatcaa aagaaattcc atctactgcc tttacacatt
     4201 taatttcctt atcaaataat ccagatttaa cttcaaagta ttttttaaga ccctctactt
     4261 ttaataaact ctcttctttt ttcaattata tctcctcctt tttatataac caacatgcaa
     4321 tactatgacc actattcaat tctattaaag gaggtatctg agttctacat ttatcacata
     4381 ctttttcaca tctatcacta aaataacatg aatctggcat cccaattgga tttggtactt
     4441 gtcctggaat cgaatacaat cttttatctt tgttttttcc aagagcaggc tttgatttta
     4501 gaagtccaat agtataagga tgttttggat ttttaaataa ttcaacaacc tctgccactt
     4561 ctataatttt gccagcatac atcactaata ctttgtcaca catttctgca actactccca
     4621 aatcatgtgt aatcatcatt atagatgtgt ttaatttttc ttttatattt ttcattatat
     4681 ctaaaatctg agcttgaata gtaacatcta aagctgttgt tggttcatca gctataagta
     4741 attttggatt acatgataaa gccatagcta tcattattcg ttgtctcata ccacctgata
     4801 attcatgtgg atatgaatta tatatttctt cagctcttgg tatttctacc agttttatca
     4861 tatcgattac ctttttctta gcttcttcct ttgataaatc ttgatgtatc ataatacctt
     4921 caatgatttg acttccaata gtaaacgctg ggtttaaaga tgtcattggt tcttgaaaaa
     4981 tcattgatat ctcattccct cttattccca taagttcatc ttcattcatc tttaaaatat
     5041 ctttgcctcg aaagattatt tctccacctt ctatttttga tgttggaagt aacttcatca
     5101 aagacatggc tgttacagac ttaccacatc ctgattctcc aactattcca atagtttcac
     5161 cttcatttat attaaaattt acttcattta cactctttac aatcccttta tctgtatgaa
     5221 aataaacttt taaatcttta acctcaatta gtgctttact cattattttc tcctctcagc
     5281 aaaattactt aagataacat acaccttttg attgtatttt ataaaaattt aaattacaac
     5341 attttcttac attttattat ttttttctat taattataat aatataatag ttaatttcta
     5401 tatatattga tttaataatg attactatta tacaataaaa aatatgtgtt aaatattatt
     5461 ttacggaggt tttataagaa tatgaaattt aaaaaattgg catcattaat cttagtttca
     5521 tcattaatgc ttacatttac agcctgtgct tccaatgata aagacaagtc tagtggaagc
     5581 aaatctggtg gaactgttgt aatacctatg gcaagtgacc cagatataat caatggagct
     5641 tttgcaaacg ttaaagaaga ctctcttgca tcaaatatag tttatgctcc tctttatact
     5701 tatgaaaaag gaaatttagt aaattattta gcagaaaaag tagattttaa agatagtaag
     5761 gaattaacta taaaattaaa atccaattta aaatggcatg atggaaagcc aataactgct
     5821 gaagatgtct tatttacatt taatacagtt ttagacgaaa aacaaaattc accatcaaga
     5881 caatacttat tggtgggtga gaaaccagtt aaagtagaaa aaattgatga tttaacagtt
     5941 aagattactt taccaactgc aagtgaatct ttcttatatg gtatatctaa aatttctcca
     6001 ataccaaaac atgtgtttga aggagaatca aatatagcta agtcagaaaa aaataataat
     6061 ccagttggtt ctggtgcatt caagtttaag gaatggaaaa aaggtgaaag catagtattt
     6121 gaaaagaatg cagattattt tggtggagaa cctaaggcag attctatagc gctaaaaata
     6181 attcctaatg aagcatctca agaagcagct ttaaataatg gcgaaatttc acttatgaag
     6241 acatctgctg aaggatatga aaaggcaaaa tcaaactcaa acttacaaac ttatacttat
     6301 agtgaagaaa gattaaacta cattgtattt aaccaaaata tatcaaacat ggctaacaaa
     6361 gaagtaagac aagccttatc atatgcctta aatagaaatg aaatgataga atcagcatat
     6421 ggaaaagaag gttctgtacc tgctaaatct atattagttc cagaagcaga cttctataca
     6481 gaagaaggtg ttgaagaata tgaccaagat acaaataagg ctaaagactt attggataaa
     6541 agtggtgtta aaattgataa attaaaaata ggttataaca caggtagatt tgggcataaa
     6601 aactatgcct tagtagctca acaagagttg aaaaaaattg gtatagaagc tgaaatagtt
     6661 ccttatgaaa gtaaggcatt cttcaatata ctatttagta atagcactga atgtgatatg
     6721 tatgtaaatg gttatgcttg gggattagaa ccaaacccat atagaggtat gtttgaaact
     6781 ggtcaatatt gcaaccaaac taaatattct aatgctaaaa tagatgcttt atgggaaaaa
     6841 ggatttactg agttaaacaa ggaaaaacgt gaagaaatat ataagcaaat acaacaggat
     6901 atatctaagg atgcgcctat ttacactata gattatgaac aaaatttaat ggcagctcaa
     6961 aaaaatctaa aaggaataaa agatgctaaa ccatctcctg caatattatt tgaagattgg
     7021 tctaaactgt atgtagaata attgatgtaa aattttatat tattaattag aaaaggagat
     7081 gtctaaaaat aatattgttt tattatttta aaacgagaca tctcttttaa ttaggagaat
     7141 ttacttatgt taaaattaat attgaataga gtaaaagttt taatacctat gttaatctta
     7201 atatctatat tatcatttgg acttttagaa ttagctcctg gtgacccagc agatgcatat
     7261 ataaatcctt taatgtcttc acaggatatt gaaaatataa gggttaatat ggggcttgat
     7321 aaaccagtat atataagata tttaaactgg ttgaaaaaca ctttaagtgg aaacttaggt
     7381 atttcttata taaaccatat gcccgttaca gaacaaatta tggaaaaaat gggaaataca
     7441 tttattctta tgggtacttc tttgatattt tcaatattgg tagctatacc tcttggaata
     7501 tttttagctg taaataaaaa ttcaataact agtaaagtat catctgtttt taattatata
     7561 ggtgtttcta taccttcttt ttggataggt atgatactca tttcaatttt ttcagttaaa
     7621 cttaacattt tcccatcagg aggtatgcat actattggaa atgatagtat tggagattta
     7681 gtaaaacatt tagttttgcc tgtaataact ttaggtctat ataatactgc tatttttaca
     7741 aattatgtag aagctagtgt aaatgaacaa ttaaaaaaac aatatgttat tacagcaaga
     7801 gcaaaaggat tatcagaaaa agtcatacta tttaaacatg tcttgaaaaa ttcactgaca
     7861 tcattggtta caatcctagg catgagtata caaaaattag tcactggtgc atttgttaca
     7921 gaagtagttt tctcttggcc aggtatggga agattaatga ttgattcaat tttttcaaga
     7981 gattatactg taattatggc aataactatg ttatcagcac tatttttaat tttaggtaat
     8041 ttagttgctg atatactata cttattaatt gaccctaaaa taaaatctag caaaggagga
     8101 ttctagttgt gtttacaagt atcttaaaaa aattaaaaaa agataaatcc tttgcttttg
     8161 catgtattac tcttagcata ataatcatat taattataac ttcttttata ttaaatatag
     8221 actctaatgc aattgatact acttctaaac ttcaagaacc tagcttaaag catatctttg
     8281 gtacagacga acttggtaga gattacttct ctagagctct ttatggtgga aggatttcac
     8341 ttagtgttgg tattttatca atgcttattt ctgtagtatt tggtactgct gtaggtgtta
     8401 ttagtggata tattggaggt aagctagacc aattcttgat gagattagta gatatgctta
     8461 tgagtatacc cagtttctta gttttgattg tattaaatac ttatctaaca cctaaagttt
     8521 ccactattat actaattatt ggattcttaa gttggatgga agtttcaaga attgtacgtg
     8581 gcgaaacatt aaaaataaag gaaaatgaat actgtctagc tgctaaagct actggtatca
     8641 agactagaaa tatactatta agacatatag ttttaaactt aaaagagact attgtagttg
     8701 ctggttcgtt aaatattgct aatgctatct taactgaatc tgctcttagt tttttaggtc
     8761 ttggaatcca attgcctctt gcatcatggg gtaatatgct tcaagatgct caaagacata
     8821 tttttgacaa gttatattta gctgtatttc caggattact tatattttta acagtcatga
     8881 gtttgactat aataagcaaa aaattttcta gtaatactta acaaatttta tatagaaaat
     8941 aaaaacagtg attatctaaa gatatacact gtttttattt ttcgtttaaa aaaatacata
     9001 ttttgtatat tataacttta aatactactc taccatgtta tgcaaagcgt attattgctc
     9061 tcaaacatat agatgaaaaa ctattagttt attataataa aaattgattt atttatttgt
     9121 tattttaata acatttttaa gagttctttt tactaaagaa atctataata aatttaaata
     9181 catgttggtc tacatggcta taacgttctt ttttaggtct acataatata actttccatg
     9241 taataggatg attaaaaggc ttttctataa ttttgctatt ttcaaaaaaa tcatgaactg
     9301 gtgatggtaa aattgttata aaatcagaat ttgtggttga tagcaacaaa aaatcccatg
     9361 aaccagacat tatatatctt tttaacttaa cattttcatc agtaaatttt tgcattagct
     9421 tatgatgtat cataaaagta ggattaaata ttgcaagtaa ctgctcattc aaatcgttcc
     9481 aatctatctt atccttctta gcaagtggat gatttatatt taaaaatgct gtaagttcat
     9541 cttcttgaag cacatgttca gttactacac ttttatcaat tttatgtgga tgtaacaata
     9601 ctgcataatc taattcctgt aagataagca ttctatttaa gtcatatgca cccttctcta
     9661 ctatatcaaa ttctatatct ggattatttg caactaactg tgcaactaca tctgaaaata
     9721 ctattccaag tataagtgga ggtattccta ttctaacttt tcctttaaat tgaactgaat
     9781 cttctcttaa atcttccatc atatttttgt attcatttat gatatgttct gcatttacat
     9841 aaaatctttc tcctgctggt gttaacccat ttaacctgcc tttatacctt tcaaataaat
     9901 atacatcttc actttcttca aaatttttta taatttgact taatgctggt tgagatatgt
     9961 gaagtgtctt agatgctatt gataaattaa aaccagattt cactatttca acaaaatatt
    10021 tcaactgttt tatatccatt ttaaaccccc ttccccaaaa gtataattta gattatattt
    10081 tttcttctaa tgatatttta taggaaaatc aacataattt cattaatatt ttaacaaaat
    10141 atttttctaa atctactata aatttttatc ctataaattt ttcttataca ctatataaaa
    10201 aattagactt agacaaaacg cttttttcgc cgtaatctat aattagaaac aggaaaacct
    10261 aaagcattag gaataaaagt aattgaaata cttaaaaaca agcttgtatt aaaaaagtac
    10321 tttattatag tgttccctaa tttctttatt cagaaaattt ttaattatca ttcaagaaag
    10381 gtaactatta gatatgaaag atgtagtaat cgtatcagca gtcagaacac ctataggaag
    10441 ttttggaggt gtatttaaaa atacttcagc tgttcaactt ggaactatcg ctgttaaaga
    10501 agcgatatca cgtgtaggac ttaatttatc tgaaatagat gaagtaataa ttggaaatgt
    10561 tcttcaaaca gggcttggac agaatgttgc tagacaaatt gctataaatg caggtatacc
    10621 aaattctgtt ccaagctaca cagtaaataa attatgtggt tcagggctta aaagtgtcca
    10681 gctagctgcc caatcaatca catctggtga aaatgatgtg gtaattgcag gtggtactga
    10741 aaatatgagc caagctcctt atatcgttcc aactgctcgc tttggtagta aaatgggtga
    10801 cataaccatg gtagatagta tgctaacaga tggccttatt gatgctttca atcagtatca
    10861 tatgggaata actgcagaaa atattgcaac taaatttgaa tttactagag aaatgcaaga
    10921 taaattggca ttagaaagtc aaaataaggc tgagaatgct ataaaaaaca atcgctttaa
    10981 agaagaaatc gttccagtag atgttttaat acgtcgagga aaaatagaaa ctattgataa
    11041 agatgaatat cctaaattag gtatgacatt tgaaggttta gctaaactta aacctgcatt
    11101 caaaaaagat ggtactgtaa cagctggaaa tgcctcaggt attaatgatg gtgcggctat
    11161 gttaatacta atgagccaac aaaaagcaga cgaactaggt attagaccac ttgctaaaat
    11221 aaaatcctat gcttcagctg gtgttgaacc tgaagtcatg ggtactggtc caatccctgc
    11281 aactagaaaa gcactgaaaa aagctggact tagcatcaat gatatagact taatagaagc
    11341 aaacgaagcg tttgctgccc aagcattagc tgttaaaaat gaacttcaaa tagattcatc
    11401 aaagttaaat gttaatggtg gtgcaatagc attaggacat ccaataggag caagtggagc
    11461 tagaatactt gttaccttaa tttatgaaat gcaaaaacgt aaagttgaaa ctggacttgc
    11521 aacactttgt attggtggtg gtcaaggaat atcaatggtg gtttcacgat aggaggaaat
    11581 atgaataaaa tagttagcat tgatgaagct ctatctcatg taaaagatgg agatacaata
    11641 atggttggtg gattcatggc caatggttca ccagaaaatt taattgatta tttatgtagt
    11701 aaaaatataa aagatttgac actaatttgc aatgatactg gctttattga caaaggagtt
    11761 ggcaaaatgg ttgtaaataa gcaatttaaa aagataattg cttctcatgt aggattaaat
    11821 aaagaaactg gaagacaaat gaacaacaat gaaacagatg ttgagttagt tcctcaggga
    11881 actcttgctg aacaaatacg tgcagctggt tatggtctag gaggtatact cacaccgact
    11941 ggtattggta ctcttgttga ggagggaaag caaaaaattg ttgtcgatag caaagaatac
    12001 cttcttgaaa aaccaattta tgcagatgtt gctcttctat ttgcatcaaa ggtcgataaa
    12061 gcaggtaatc tagtttataa aggctctatg aacaatttta ataatttaat ggcatctgct
    12121 gccaaaatta caatagttga agcagatgaa atcgttgaaa ttggtgatat tgacccaaat
    12181 gaagttaata caccaggtat ttttgtgaat tacatcgttg aaggaggtaa tttataatgg
    12241 ataaattaga aatgcaagaa tatattgcaa atagagtatc taaagaatta aaagacggag
    12301 ccgttgtaaa ccttggaata ggtctaccaa ccaaagtagc aaattatatt ccggataatg
    12361 taaacgttat cttacaatct gaaaatggct ttttaggttt aggttcagcc gaagatggaa
    12421 attcaagtga tgaaactatt gtaaatgcag gtggtcaacc agttactata cttcctggag
    12481 gatgtttctt tgatagtgct acttcttttg gaataattag aggaggtcat gttgatataa
    12541 ctgttcttgg cgcacttcag gttgacaaat atggcaatat agcaaactat atgattccag
    12601 gtaagatggt tccaggtatg ggtggagcta tggacctagt aactggagct aaaaaagtta
    12661 ttgtagcaat ggaacatact tcaaaaggtt ctgcaaaaat attaaataat tgtactcttc
    12721 cacttactgc aacaaatgct gtagatttga tagttactga aatgggagtt atggaagtaa
    12781 catcagatgg aattttactt aaagaaataa atccagcttt cacattagat gatgttattt
    12841 ctgctactga agctccttta atattgtcag attcattaaa tggaaaaata tctgttgttt
    12901 aaaataatta aaactagaaa ataaaaatct cttagacgta ttacatataa atttgtattg
    12961 cttaaaatat taatataaaa ataaaataaa attggaggaa atgaaaatgg ttaaagataa
    13021 agttgtattc gtaacaggag ctgcaagtgg aataggtaaa caaatagctg aatcgttctt
    13081 aaaaaatggt gctaaagtaa tgttctctga tataaatcaa gaagctttaa ataataccat
    13141 aacagagtta caaaaaaaag gttatgattg catgagtgta aagtgtgatg taactaagga
    13201 agaagaaatt aaccacgcta tagacaaaac tgttgaaaag tatggcagat tagatatatt
    13261 aataaacaat gctggacttc aacatgtttc tatgatagaa gatttcccaa ctgataaatt
    13321 tgaatttatg ataaaaataa tgctgacagc accttttata gctactaaaa aagcatttcc
    13381 tataatgaaa aaacaaggct ttggtcgtat aataaatatg gcctcaatca atggtgtaat
    13441 aggatttgct ggaaaggctg cttataactc agcaaaacat ggacttattg gactaacaaa
    13501 agttgctgct cttgaagctg ccgattctgg tataacagtt aatgctttat gccctggata
    13561 tgtagatact ccacttgtca gaggccagtt caatgacctt tcaaaaactc gtaatatacc
    13621 attggaaaat gtcttagagg aagttttata cccacttgta ccacaaaaac gcctattaga
    13681 tgttcaagaa attgccgact atgctatgtt cttagcaagt gataatgcta agggtattac
    13741 aggacaagct tgcttactag atggaggata tactgcacaa tagttattat atttagggct
    13801 tgaagaaagt attcttcagg tccttctact aaggagggat atttatgagt attatcgcgc
    13861 caattggtat aattattggt atcatagcta ttatatattt ttcagttaaa gaaattcata
    13921 taactatagc tgctccaata gcaactttaa tagttgttat tttaaataaa atggatgttg
    13981 taacatcaat gctaggtgct gacaaaaaca attatatggg tgcacttgga aactatataa
    14041 tgagttattt tgcaatattc ttattaggct caatacttgc taaatttatg gaagaaagtg
    14101 gagctactgt atcaatagct gattttattt taaataaatt tggctctaat catccttata
    14161 gagttttagt tgctatattt atagtaagtt ttatattaac gtatggtggt ataagcttat
    14221 ttgtagttat gtttgcggtc attccacttg caagaactct atttaaaaaa cttgatattt
    14281 cttggaacct tattcagata ccactttggc ttggtattgc aacaattact atgactataa
    14341 ttcctggaac accagccatt caaaatgtta tacctattca atatctaaat acgtctctta
    14401 ctgcagctac tataccaagt attgtaggta gtataggttg tgctacgttt ggacttgtct
    14461 atatgaaata tgctctaaac aaaagtatta gaaaaggtga aacttatgca acttatactt
    14521 cagaattaga agaagaatct ttagatagag aattaccagg attttttgca agtatttttc
    14581 ctctattagc actcgtaatt atagctataa ctggaagtac ttttggttca gaatttatga
    14641 aaaaaaatgt aatttacata gctttaatag ttgcaattct tttatctgct tttttattcc
    14701 ataaatttct atcatctaag atacagacat tgagcatagg tgctagtggt tctataggtc
    14761 ctatatttgc tacatcttct gcagttgcat ttggagcagt aataatggct gcacctggtt
    14821 ttgatgtatt tagcaaactt ataatgtcaa taccaggtag tccacttata agtcttactg
    14881 ttttgacttc aacaatgtca gcaataacag ggtcctcttc tggtgcatta ggaatagtaa
    14941 tgccaaactt tgcagattat tatttaagta ctggtctaaa tccagaatta attcaccgtg
    15001 ttgctacaat agcttcaaat atactaacta tagttccaca aagtggtgtt tttcttacat
    15061 tcttaagctt atctaagtta acacataaaa ctggatttaa agaaactttc attgtagttg
    15121 cagttggttc actaatagca gaaattattg taattacatt aggattacta atgtattaat
    15181 ataaaacaaa ttaaaattca taatatgaaa ggtggtgtct caaagtgaac tttttactta
    15241 atgagacact ctttttatat gaaataacat atgcctatca tttcaacaat aaaaaaggcc
    15301 ttatctctat tttgagacag acccttttta tttcttaaag atttaaataa ttataatttg
    15361 ttgctaatac gaaagcatct actgaataaa tttaaccaaa taaaaaaaaa tttatcataa
    15421 ctataaattt acgttatgga attattataa aaatattatt ttctctaact aagtaattcc
    15481 atactataca ggcttttaat acaaattgtc aaacacactg aaatatacct atttattcca
    15541 tatatatctt ttttacatat ctttacagta aaaaaagtct taatcttatg ttagtataga
    15601 ttcacgggta accccctaca ctaaaggtta caaatcagta acatttttag aatttttaga
    15661 ttaagaggtg tttaatgatg agaagaaata taccaacaaa attaaaaact atattagctt
    15721 taggagtgtt aactttagca acagcttcaa gtgctgttac tgcaagtgct tcatcacttg
    15781 aaaataatgt aaatataagt aacaaaagtt tagtatatag taatactaaa tacaagtttt
    15841 ctgcaaaacc aaattgttct gtaaaaccag aacttaagcc tgataaagat aactcttgta
    15901 aggataagaa tcataatgac aagaattgca acaacactaa taaacctgag gacaacaata
    15961 attcagactc tactaataag cctgataaca ataaacctga gtctaataaa cctgaagaca
    16021 acaataattc aggctctact aataaacctg agtctaataa acctgaagac aacaataatt
    16081 cagactctac taacaaacct gatgataata acaactcagg ctctacaagt gaaagttttt
    16141 cagcttacca aaaagaagtt gttgacttag taaatataga aagagctaaa gctggattaa
    16201 atcctcttac tcttgattct tctatatcaa atgttgctac aaaaaaatct caagatatga
    16261 ttgataacaa ctatttttca cacaattctc caacttatgg ttctccattt gacatgttga
    16321 aaaaatttgg aataagctat aagacagcag gtgaaaatat agctatgggt caaaaaactc
    16381 ctaaagaagt tgtaaatgca tggatgaatt ctgaaggtca cagaaaaaat ataatgaatc
    16441 caaatttcag taaaataggt gttggagtag cacaaaaaag tggtggttca atttattgga
    16501 cacaaatctt tgtaggataa aatactcttt ttccatacat atcaaaagag tatctctaag
    16561 tgataaaaaa cacaaatgag atactctttt gtatctattt taaaattatc aaatccaaat
    16621 atacatacta taaatattat ttgttctctt aattttagtg tgtagagcaa tatataaaaa
    16681 gtattggcaa gatgaaaatg gaaaacttaa aaagatattt aataggtaaa aacaggatct
    16741 taatatcttc ataaaaaaag gctgtctcaa aatagagata aggccttttt cattgttgaa
    16801 atgattaaaa tatatttgat ttcatacaaa aagagtgcct catgaactaa aaagctcact
    16861 ttgagacact ctcttttatc aattctatat aagcattata atactttaat attattcagc
    16921 taattttttg tatccttcat atctttcttt agcatcagct tcagctttag caaataatcc
    16981 atcagcttcc tctgggaatt gtttagctat agcagaatat cttacttgtg caagtatgaa
    17041 atctttgaag cttgcagttg gttcttttga atctaaagtg aatgggtttt taccttcagc
    17101 ttttacttct ggattgaatc tgtataaatg ccagtatccg caagcaacag cttgtgcttc
    17161 gttagctaca gttcttccca taccttcttt taatccatgg tttatacatg gagcataagc
    17221 tattattaaa gatggcccat catgtttttc agcttctata acggctttga taaattggtt
    17281 tttatcagca cccatagcaa cttgagctac atatacgttt ccatatgtca tagccatcat
    17341 tcctaaatct ttctttctag attttttacc tgaagcagca aacttagcca tagcagcagc
    17401 tggagtagct ttagaagctt gacctcctgt atttgaatat acttctgtat caaatacaag
    17461 tacgtttaca tcttcaccag atgcaagaac atggtctaat ccaccgtaac cgatatcata
    17521 tgcccaaccg tctccaccta gtatccattg agatctctta actagataat cttttctatc
    17581 ttttatagct tttactaact ctttagcttt ttcatctgta caatcacaag ctggtttttc
    17641 taataattca acaaccttag cacttgctac tttagaagct tctccatcat ttctagaatc
    17701 taaccactct gtaaatactg ctttagcatc ttcacagata cccatagaaa ctaactctgt
    17761 catagcatct actattttat ttcttatttg cttaacagct gtgtacatac caaatccata
    17821 ttcagcatta tcctcgaata aagagtttgc ccaagatgga cctttacctt catggttaca
    17881 agtataagga attgatggtg cagaagctcc ccatatagaa gaacatccag tagcattagc
    17941 tatcatcatt ctatctccat ataattgtgt aactacttta atgtatgcag tttctccaca
    18001 tccagcacat gctcctgaaa actccattaa tggttgtcta aattgacttc ctttaacagt
    18061 gtttacaggc ataacatcac cttttggagc tacctcatca ggatttactg caaatgccca
    18121 attatctaac tctgtatcaa gttgagtatc tattggcttc ataactaaag ctttttcttt
    18181 agctggacat atgtcagcac agtttccaca tcctgtacag tcaagtggac taagttgcat
    18241 tctatatttt aatccttcta atcctttacc taatgctttt ttagcttcaa atccttctgg
    18301 agcattagct aattcttctt cagtaactaa tactggtctt atacatgaat gaggacatat
    18361 aaaagaacat tggttacatt gaatacagtt atctattatc cattcaggaa catcaacagc
    18421 tataccacgt ttttcataag ctgcagtacc acaagggaat gttccatctt ctataccatt
    18481 gaatgtactt actggtaagt tgtttccttc ttgtgcagtc attggtctta gtatattttt
    18541 tataaattct ggttctttaa cttctccttt atctgcatca acagcatttt cccaagaagc
    18601 tggaacttca actttaacta aagcatttat tcctgcatca acagcagcat agttcatgtt
    18661 aactattttt tcaccttttt taccataagc tttaactata gaatctttta agtatttaac
    18721 agcatcttct tctggtatta tttcagctaa tttaaagaat gcagattgca ttatcatatt
    18781 tattctgttt ccaagaccta tttcttgtcc tagtttaaca gcgttaactg tatagaattt
    18841 tatatcattt ttagctatat attgcttcat atgagctggt aaatgttctt caagtcctgc
    18901 ttggtcccat atagtgttaa gtacaaatgt accacctttt ttaagacctt ttaataaatc
    18961 atattgataa acatatgatt gtttatgaca ggcaatataa tcagcttcat ctattaaata
    19021 agtagctctt attggagtat caccaaatct taagtgagac atagttatac caccagattt
    19081 tttagagtca taatcaaagt aagcttgagc atatttttca gtatggtcac caattatttt
    19141 gatagcttgt ttattagctc caacagtacc gtcagaacct aatccccaga acttacatct
    19201 tatagttcct ttagaagcta tttttattgg ctcagatgga gttaaagaag tattagttac
    19261 atcatcaact atacctactg taaatccatt tttaggttgt tctgaaacta agttatcaaa
    19321 aacagttttt atatctgaag gagtagtatc ttttgaacct aatccgtatc ttccacctat
    19381 tatcatagga gcattttctt ttccatagaa tacatcacgt acatctaagt ataatggttc
    19441 accagttgaa cctggctctt tagttctatc aagtacacat attctttcaa cagtagatgg
    19501 catagcatct aagaaatgtt tcattgagaa tggtctatat aaatgaactt taactaaacc
    19561 atatttttct cccttagcat ttaagtaatc tatagtttct tctattgttt ctgttactga
    19621 acccatagct attaatacgt atttagcatc ttctgctcca tagtaatcaa ataaactatg
    19681 ttttctacct gttaaattac tcattttttc catgtatttt tcaactatac ctactatatt
    19741 ttgatagtat ttattagaag cttctctagt ttggaaatat atatctgggt tttgagcagt
    19801 accacgagca actggatgat ttggagataa agcattatct ctaaatgctt gaactgcttc
    19861 aaagtttaat aaacttgcat aatcttcatt ttctagtaat tcaacttttt ggatttcatg
    19921 tgaagttctg aatccatcaa agaaatgtat aaatggtaat ctaccttcta ttgcagctaa
    19981 atgtgcaaca ggtgctatat cagctacttc ttgaactgaa ccagaagcta ataatacaca
    20041 tccagtttgt ctagcagcca taacgtcttg gtgatcccca aatattgata acgcttgaga
    20101 tgctaaagca cgagcactta catggaatac tcctggtaat aactctcctg caactttata
    20161 catgttaggt atcattaata ataaaccttg agatgctgta aaagtagaag ttaaagctcc
    20221 tccttgtaaa gaaccgtgga atgttcctga tgcacctgct tctgactgca tttcaacaac
    20281 attaactgtt tgtccaaata tattttttct tccttgtgat gcccactcat caactacttc
    20341 agccatagtt gaagatggtg tgattggata tatagctgct acatctgtaa acgcataggc
    20401 aacatgagct gcagctgtat ttccatcaag tgttttcata aacttagcca taatatgtat
    20461 cctccttgat agctacaatt gtacttgttt ataacaagtc tattttttta tatttacata
    20521 taatttacac aacaaaacta taaacacaca tatagtcgcg caaatactct atcttctaat
    20581 ataatactac agattagata aaattccttt gaaaattgta aattaattac atatttaagt
    20641 agattttttt taaaattaaa tatgttaagt ttttttcata attttgtata caatatataa
    20701 taaattaagt tagatttttc aaactttttt atctatttgt tcatattagt ataaaaagta
    20761 aaatgcttat ccttattaca tgtaaatttt tatatatata attttaccat attacatcat
    20821 atttttgtaa gtttttatta cattttttat aatacccctt tattttataa attaatctaa
    20881 tcgattaaat ttatcctata gaaatttatt acatttaaaa atatgctagc aaataaaata
    20941 tctactagca taaagtctat ttataatatt aatcaactat taattctact aaatctccat
    21001 ttttaacgcc tgctgcattt ccctcttcca tatcaacatg catttctaga ttaaagctgt
    21061 cactagctct tactaataca ttttcaaata ctaatgctct tggtccagat gttttaactt
    21121 ttactatttg cttatcagtt acaccaaatt tagcagcatc ttcattgctc atatgtatat
    21181 gtctagaagc aactataact ccttctttca tttcaacttc tccagcaggt cctactaatt
    21241 taacacctgg agttccagct atatcaccag attcttttat tggtgctttt actcctaatg
    21301 ggaaaccatc tgctaaagat atttcaactt gagtatgagc tctagctggt cctagtactc
    21361 taactccttt taaagttcct tttggtccta ctaaatccac tttttcttca caggcatatt
    21421 gccctggttg agataatggt ttaaatttag ttaactcatg acctgcccca aataatacgt
    21481 ctatatcagc ttgactcaaa tgaacatgtt tattagataa tgctattggt aatttcattg
    21541 aaattccctc cttttaatta tttaaattat tgcaatacaa ttatacataa tattacctac
    21601 aaaatcaaat tatattgaat caatatatat ctttaaattt ttatatatta tatctttaaa
    21661 ttcttgatta tctatataag atacatcatc tgtattgttt aaatatatat tgtgtaaaag
    21721 tttagtattt atactcttaa agaaaaaatc catcacaatc tgtccacctt taaactgtga
    21781 atcatatgat ttactccccc ccactgcaat atacattcct atccgtttct tacttctatc
    21841 aattgaaggt ttatttaata catatttact tgcatatata gcttgagttc tatcaacaac
    21901 agtctttaat ttagctgata ttgaatcaaa atgtacagga cttattacta tagttccttt
    21961 tgaatcatca aacattttat ataatggctt catatcatca ttaaacttgc aataccctgt
    22021 tttttcacaa aatccacatg ctgtgcaata ttcaatatcc atattgtaga tattaaatat
    22081 tttacaactt aatccacact cttctagcat agcttttact tcatttgcta taaaactgca
    22141 atttttatta attcttggac ttccatttat tattataatc tctggacttt taagtccttt
    22201 tttatcgttt gaatccataa tcaatctacc tatcctttaa cataactatt aaaatatcat
    22261 catcataatt atcttttgat gagaagttct ttaaatccaa ttttagattt tctactatta
    22321 actgttgatt taatctaaaa ttttctttta gaaagttttc taatcggttt ataccatact
    22381 cttcttttga tgaatttttt atctctaaaa ttccatcagt gtacatacat accattgcat
    22441 aatcttccat tgaaagagta ttattttcat aagtagcatc ttccattatt ccaataggta
    22501 tgcccttttt acaatttaaa ttattttcta ttgtgccatc tttctttaca actacaggac
    22561 tatagtgacc tgcattagac acagtaatca tattttcttt agtatctata atacctatca
    22621 aacaagttgt aaatactccc atcttgtcaa attcatcata taacatccta tttaaatttg
    22681 tcattatttc tcttggtgta ttgtattgat tgcataatac tttaaaagca ccttttatca
    22741 ttgcaacaac ataatttgat accattccat gtcccattac atcagcaaca atataaacta
    22801 tatgttcgtc atccactttt gtagcataat aaaaatctcc acctactacc cttgctggtt
    22861 tatggtaaaa ataaagttct ttctcatcat tacaatccat tttaccttta tccataataa
    22921 gctgttgttg tttatttaat atgttcaact cgttttcaag aactttgtgt tttacttcct
    22981 tttcattatg ttcatataat tgcataccaa cagaaacttg tttggacaat atactaagaa
    23041 tatttaaatc atcactagta tagtttttgc tatttgcaca aactatacat cctatagtat
    23101 catttttatc ctgaagtttg taatatatat aactttccaa atttattccc ttttttgagc
    23161 atggttttaa attatttaga taccccccca atgactcttt ctttaaaaac atatcaatca
    23221 taggatataa atttgttttc tttttttgct caacattata acaactaact atagcatcct
    23281 tattatgatg actatttata attattaaag cttctttact attagtaata ttacacgctt
    23341 gtttacttaa attttccaaa aagccatcaa tactcttatt aaggtatagt tttttagtag
    23401 cttcatttat ggcacttatt gagcctctta gtttatcaat taaaatttgt acattattat
    23461 ttttattagt tatttctatt gataaactta agagtgatgc aacagatgat ataaaatcta
    23521 tatcttgttc tgttatataa tcatcctctt ttaaaaaaca agtcataaat ccaacaacta
    23581 cgttatttat tataaaagga aaaactattc tcccaatata accttcagct tttgctaaat
    23641 ctcgttcacc ttctgctcta gaatcttcaa atacattttc cacatatatt atcttttttt
    23701 catttacagc ctcatgtata tactctggat atgtgtcaaa atcaattttt gcacctttaa
    23761 gactatttat tggaaaaatt tgaggtacat attctaaggt ttgcgaacat actaagtagg
    23821 catatttaga atcattttta taaaatagat tcacacatgc ctttgtaggg tgaacaactt
    23881 ctaacatttt ttctattatg tcatctttta tattaaaaaa atctgtagat tcagtcacca
    23941 tagcggatat ttccactaaa ttcttcattt tataactgcc attagtcata ctcaatttct
    24001 ccccctatat attaaactat ttattagtat tttaacatac attctttata gtaaaaacaa
    24061 aaacaacgac aaaactagca actaatatcc agttaaatcc tgtcaataat cctataagaa
    24121 gtaatgtaat taatgcattt agacctgttc tccctttttc atccataaaa gttattttag
    24181 agatgtcgat attatttttc acattattta tatgctcttt cacttgttct tccatatttc
    24241 ttttatatag tatataaact acaccaacta aaaacactcc tatacctaaa accatgttta
    24301 aattaaaagc taccataagc aatattgaac caaaagctaa ctttaaaagc ttaaaaaata
    24361 cactatctat ctttagagtt atatttctga tttctttaca ttcatctctt aaagaatatc
    24421 cacttgaatc taagttatta atctttgttt ttacaatttc agaataaaaa agctttatta
    24481 tcccatttaa cattttcatc actcctaaat attttacata tatttgtaag ttttacaatt
    24541 ataatatttt ttatacttgg taataataaa aaataacaaa tattttatta tttagtattt
    24601 attgtaaata agttattgaa gcttgattag ttaagtatag atttttaata aatattttta
    24661 taatttagaa ggaagtggtt ttgtgcaaat agaacaactc gatttagaaa cgagaaataa
    24721 aatatactgc tatactaaaa aaattcttag aaaatatcaa aaaggcataa catctggaaa
    24781 attaacagca gataaatttg cagataatat attgtctcaa gattttatca gtagtatact
    24841 taatgaaaaa atagtcaatg agacaaactt taagatatct tatagaaact atatagaaac
    24901 tttaataaat attcaaaatg aaaatttatc taacttaaga aaaaaatcta ctaaaactgc
    24961 aaaatgtttc aatatctctc aaataaccca attaaaaaac ttactaagta atactggtta
    25021 taatttatta attccatata aatacttaac tgctagagat atagaaggaa tagttactct
    25081 tattaatact gggtctattg aacttggaaa tgagagaatc tacaattata tatcaaaagt
    25141 ataataattt aaacctatta taaaaaggca atttatttaa taaaaaatat ttttaatatt
    25201 aaataaattg cctttttaat tttggattaa atactgctat ccaagtgctt tttctagttt
    25261 agataaatgt tgtattggat aatattttag tagctcttta aattgttcta ctgataaacc
    25321 ttctgttgtt gtatatattc ttattttttc atctaagtca ctattaataa atttgttttt
    25381 taaatcctca aaatttacac tactcatttt acatcactcc ttatgtaaca tattcatttg
    25441 tattatttta tccaaataca taaaatttat tacataaaaa tatatctatt taataattta
    25501 ataaaaaaaa gctatctcaa tttagaaatt actttctata tttgagatag ctctagctaa
    25561 actaatagcc aatagttata aataattatt tataactatc tgttgctttt tccagccatt
    25621 tgttgttcag ccatttcaac taatttttta gtcatgtatc cacctacata accattttct
    25681 cttgcagtaa ggtttccttt atcaacttgt tgataattac tcataccaag ttcattagct
    25741 atttctaatt tcatttggtt taaagcctct tttgctcctg gtactactgt tctgttgttg
    25801 ttattgttac ttgccatgtt gattaccttc cttctgttta atttattttg tgttgctgat
    25861 aatattatgt gattttaaac atttttaatt caggtaattt ttagcaatta aaacctgcca
    25921 aatatccagt agaaaaagct atttgcaaat tgaatccacc tgtatatgca tctatatcta
    25981 ttacttctcc agcaaaaaat aaaccttcta ctaattttga ttccattgta cttgaattta
    26041 tttcgtttac ttttacacca ccagaagtta ttatagcttc ttcaataggt ctgtatctct
    26101 ttactgtaaa ctttaaattt tttaataaat ggactaagtt ttttctctgc tctctagaaa
    26161 tttggtgaac aaccgtattt ggattaattt cacttaagtt tattattata ggtattagtt
    26221 tttttggaag taaatcatct aatgcttttt caaatttttt atttgtatac ttctgaaaat
    26281 ctttttgaac tcttttatcc aatttttcct catctaatgc aggttttaaa tctaataata
    26341 tagtgtaatt ttctttcctt gtatctttca ttctacaact tgcagacttg attataggtc
    26401 catctaaacc aaatttagta aattcaagtt ctccaaaatc actataaact ttcttttgtt
    26461 tgctattaaa cacccttatt tcaacatttc tcaatgataa tttttctaaa tcttttgtaa
    26521 aactttcttg tacttctata ccaattaaag atggttttgt atctataatt gtgtgcccct
    26581 gtgaaattgc aaatttatat ccatcaccag tagaaccagt aagtggataa cttaatccac
    26641 cagttgctac tacaactgaa tcacacttaa tttctttttt atcgtttaaa attacttttt
    26701 caattttatt atttttagat attatttttt ctactttcga atttaataat atatttactt
    26761 tcttactttt aagttgtctt tcaagagcat ttacaatatc aaatgcttta tcactctctg
    26821 gaaaaaccct ttttcctctt tctgttttag tctttactcc aagattatta aacatactta
    26881 ttacatcatc atttgtaaaa gtataaaaag cactataaag aaattttcca tttgtaggta
    26941 cattttctat taattcctct atttcacaag cgttagtaat attacatcta ccttttcctg
    27001 ttattgcaag tttttttcct aatttatgat ttttctcaat taatgttaca tcatagcctt
    27061 tctcacatgc agttgatgcg gctatcatcc ctgctggtcc accacctatt attactacct
    27121 tcttcaaata atcactcttt tctaccctta atttttttaa tttaaactat tgactctata
    27181 ccttaaaatc aatttaattt taaaagttta aataatgctg gagctcctgc tattccataa
    27241 aaaacaacta ctgtattctt aataaaattc ataaaaatta tactcatagt agagtcactt
    27301 atatttaaag tgactattaa cattgaaata aacaacttaa tgccaatata taacattcct
    27361 attgttacta tcccaaccaa aaacctacat attcttgaaa aaattatatt tttactcccc
    27421 ttatttttat tgctaaaatt tatttttttg ttattattat ctgtattaaa tttaacaaat
    27481 ctatcctcaa ccatatatcc aaatacaaat cctactatta taccaaattt ttcaaaatat
    27541 tcagggctat ttagaaatat agccactatt ataaatggta taagtaaaac tacaagtaaa
    27601 atataatttt tattatcatc aatatagtca aatattttta caaatacaac tgtaaaaaat
    27661 attcctaata tccaacccgc tatcacatct attggccaat gaacagctaa atacagccta
    27721 gacaatccta ctcctaaaac cataactgca cctattaaat gaagatatct ctttttaaat
    27781 atagtcataa tactaaccca aaatgttgtt gcagtttgag tatgagcact tggaaaagaa
    27841 taaccaccag cagtagaaac tctcaatgat tcaagacctt ttattcctat tggtctggga
    27901 gcttttacaa actctttgat gcctaaattt acaacaaaat ttccaattat agcaaacagt
    27961 attttctgtc cacatttttt atttatacac cagtatataa ttgttgtaat taatattaat
    28021 aacggtgctt ctgtgcttat tgtaaagaca agaaatataa agttcaaaat tggatttctt
    28081 aaattttgga aaaataataa tatatttaac tgaatatcca tatagatgtc tcctttacta
    28141 aaattttata taattaacta aaaagcacta actcccttag aaattagtgc ttttgattca
    28201 catttaaatc tattctacat cctcaactat acctatataa ggtaaatttc tatacttttc
    28261 agcatagtct attccataac caacaataaa tttgtcttct ataacaaatc caacatagtc
    28321 tacaggtata ttagcttttc ttctctgagg tttatcaagt agagtacata gttttaaaga
    28381 ttttggattt ctagttttta aagctgcaac tagattactt aatgttaaac cagaatctat
    28441 tatatcctct acaatcaaca cattctttcc ttcaatatct acatctaaat cttttaatat
    28501 tttaactgtt ccagaagact ctgttgagtt tccataactt gtaacactca taaaatctat
    28561 atttacatcc aaatctatac atcttattaa atctgaaaca aatacactag ctcctttaag
    28621 tatacctacc actagtaaat cttcgccttt aaagtcttct tctatctttt ttcctaattc
    28681 ataaactttt tcttttattt gttcttctgt caacattttt cctgtaactt tgtacatttt
    28741 gtatcctccc gctttccttc tattaactta tatattatag cataccaaaa tatcaattaa
    28801 aatctttttc atcattttta aacctaaatt aacagcttat ccatactttt ttgcttttta
    28861 cattaaaaat ttggaataac gattcctttg tattataatc tatatgtaca tatattaatc
    28921 agttattttt atcgattata taattttatt tttggaggta cttttatgag cacaaaccat
    28981 ggagctaatt tatatagttt atctagtaag tatggctttt caaaggaaga atttatggat
    29041 tttagctcaa atataaatcc ttttggtaca tcaagtttag ccaaacaata cattgtaaac
    29101 aatatagata tggtatctat gtatcctgac cctgattaca tagacttaaa aacttctata
    29161 tccaattact gcaaatgttc tatagataat atagtacttg gcagtggagc tacagaacta
    29221 atttcataat ttatacatac aattaaccct aaacaggctc ttttactctc tccagcctat
    29281 tcagagtatg aaaaagagtt atctaaaatt aattgttcta tagaaaaata ctttgcaaaa
    29341 gaagaagata attttcatat aaatttagaa aatcttataa aaactatcaa tgctaaagat
    29401 tatgatttgg tagtaatctg taatcctagt aatccaactg gatttgcatt tactaaagta
    29461 gaagtgagag aaattttaaa aaatacagac tcatttttaa tgattgatga gacttatgta
    29521 gagtttactg atacagatac atactcttgt actcaattag ttgatgatta ttctaattta
    29581 ttcgttataa gaggtacatc aaaatttttt tcaacgcctg gaataaggct tggatatggt
    29641 cttatatcta acactaatgt caaaaatgaa ataaataaaa atctagattt atggaatata
    29701 aacataatag cttctaaaat gggtgaaatc atgtttagtg atttagattt tatatcaaat
    29761 actatttcct taatgaacac tgaaagagat tacctgctta aagagcttaa gaatataaaa
    29821 agcttagatg tatacaatac taaaggtaat ttcatattgt gtaaaatcaa gacaaaggaa
    29881 ttgactgcta aatctcttcg agagcaactg ctcccacaaa aaataataat aagagattgt
    29941 tgttcatttg aaggtctaga tgagtatttc tttagagtat gtatattaaa acctaatgaa
    30001 aataaattat taatatcatc tcttaaagca atttttaaat aaaaaaatca ctttatttta
    30061 taatttttta taccataaaa aatgagctat ctctatttga gataactcat ttttgattat
    30121 taaaaaacta agcactttgc tcttctttgt ttcctttttt actcattcca tcatttattt
    30181 tatctactac tactgctatt gcattatctc ctgatacatt acatgctgta ccaaaactgt
    30241 cttgagttaa gtataaagct atcattaaac ttgcaagccc accatctgat ggtattccaa
    30301 ccattggtag gaaaggaagt gcactcatta tagccccacc tggtgctcct ggtgctgcaa
    30361 ccattgctat acctaacatt gcaatataag ggaataatac acctattcca tgtggcatac
    30421 cattcatcat taaaactgca gttgtaaatc ctgttattga tatcatacta cctgataaat
    30481 gtattgttgc acaaagaggt acaacaaatt ctcttatttg cttacttact ccattttttt
    30541 ctgcacaacc aacattaact ggtatagttg ctgctgaaga ttgagttcct atagctgtta
    30601 aatatccagg tacttgattt tttaacattc ttactggatt ttttcctgaa aatacacctg
    30661 ctgctgtgaa ttgacatgcc atatatacaa aatgtaaagg tattactact aagtatactt
    30721 tccagaatac ccctaatatt gtccatactt cccctgctat agttatattt gcaaaagttc
    30781 ctgctatgta taaaggtaat aaaggtataa tcactgttga caacgttttc gttacgattc
    30841 cttgaaattc ttggaagaaa ttaaataaag tctctccttt tccatgattt cttaaagctg
    30901 atattccaag tcccattaca aatgcaaata cgattgcagc tgttacatct accataggct
    30961 ctaatggaat tgtgaatatt ggagctaaca ttcctgcctc tgggtctcca atttgacttg
    31021 caacacttgc atcaataaaa tttgggaaga ttaatgtaga tactgcaaat gataataatc
    31081 ctgctattaa ggtagaacca tatgctattc ccgtagttat ccccaataac tttcctgcac
    31141 ctgttgctaa atcagctata cctgctgtta caaaacctat aattataaat ggtataacaa
    31201 attttaggaa tgctgagaat aaagtactaa ctgttactaa tatttgtact attatttctg
    31261 gtgcataact tccgattaaa ataccgacta tgataccgat gattagtttg ggcactaaac
    31321 ctattttttt cttcataata tttaccctcc ataacttttt taaattaagc attcttgcac
    31381 attgaatact tagttaaata ttggatatcc acttattatg ataatagagt tttacctctc
    31441 cgtcaacaat ttaaccattt tttgttaata aaaaaaagaa taacatatat aaattgttat
    31501 tcttcttcat cttttataga aaattctaca ttttcaatta tatttgaatg tttatctttt
    31561 tttgcgataa ctctatactt attaatttct tcattcacta tacctatact ccaaattaaa
    31621 actatagcat catctataaa accaaatgcc cctgcaatca tctctggaat tatatcaacc
    31681 ccagatacaa agtaggatat agtaaaaatt aatacagaaa ttacctttat tttatcaact
    31741 atattgattc ttttatctgt cataaaatca ggtagtttaa gtaaatcaaa tacaaaaacc
    31801 aaaccaaatt tagcattttt aaatctgacc aaaatttttt ttaaaatatc taaaactata
    31861 ttaatgatta tttttaaaaa ctccatattt atcacctctt ataattatag gttattctac
    31921 attataaccg atataattaa tttgatgaac atattagata taaattttat caattagttt
    31981 atatttaaat tataataatt caattcctgc actactacaa ttttgaatca tatcctgtgg
    32041 ccatactcca acttgtactt ctcctatatg agctttttgt aagaaataca tacatattct
    32101 tgactgacca attcctccac caattgtata tggaagttcg ccatttaaaa gcatcttatg
    32161 atagtcaaac tcttttctct cttcacatcc agcaagtttc aattgtctat caagagattc
    32221 ttcatcaact ctaattccca ttgaagataa ttctaatgct gaatctaaaa caggattgta
    32281 aaataatata tccccattca attcccaatc atcataatca ggacttcttc catcatgttt
    32341 ttctcctgat attaatgttt ttcctatttg agttataaat actgctttct tctcttttac
    32401 aattttatct tctctttcct ttggtgataa ttctggatac atatcttcta actcttgtga
    32461 tgttataaaa gttatttctt ctggtaatat ttcttttata ccttcataca tactacatac
    32521 aaaaatttca gtatctttaa atactccata caatcttttt acaacatctt ttagagtctt
    32581 ttcatttcta ctacttttat ctataaccaa ttcccaatcc cattggtcta cataaagaga
    32641 atgtatattg tctaattctt cattttttct tattgcattc atatctgtat ataaaccact
    32701 accaacttca aacccatatt ttttaagggc taatcttttc cattttgcaa gtgattgaac
    32761 aatttctgca tttttattca taaatggtat atcaaagctt acagcctttt ctccatttaa
    32821 attatcattt gcacctgttt ctggcaatac aaataacgga gaagaaactc ttataagatt
    32881 taattcatta cttagtctca tttcaaaaaa atcttttaat ttttttatag ctatttgtgt
    32941 atcaattacg ttcaaactac ttttataatc ctttggtatt attgtgctca tatcattcac
    33001 tccctatatt tatatatttt tccattaaaa aaacccttca tccctatata aaagggacga
    33061 aaggttgctt ttccgcggta ccaccctgat tagtttgaaa taaattcaaa cccagctcac
    33121 aatgtacaga tattactcga tacaccccaa tatgataacg gtttggttcc gactaagcct
    33181 actcttttaa aatttcggtt agcaactcca ggatgttctt cattatagac ttcatattgg
    33241 tctcacacca tcaccaactc gctttaatta cattctataa ctactcttcc tttcaaagtc
    33301 tttaagagtt taattcaata aaatttagct ctcttatgtt ttctatttat tcataatagt
    33361 atgataagtg attagttaaa atttgtcaag tttattattt ttttattttt aaacacttta
    33421 taaattttta cctatacttt ataaattttc aaatatagtc tttaatctgt ccatacattc
    33481 ttttaataaa gactgtggac aagctacatt tattctttca aatcctattc cttcttttcc
    33541 aaatatataa ccttcatcaa ataatacatt agctttttta aacattaaat cctcaagttc
    33601 tacttcattt aatccataag cagtaaaatc taaccatgct agatatgttc cttctggata
    33661 aacaagtttt acttttggta ggttttcttt taagtattca tcaataaatt gtatattatt
    33721 atctaagtaa tcatttaatt cattaagcca gtcttcactt tcattatatg ctgctattgt
    33781 agctacaata gcaaagggat ttggtgaagt tgcaactcca acagctgtaa cctcttcttg
    33841 atatttcttt tttagttcat cattattcgc tattatatta gacaactgca ttcctgccag
    33901 attaaatgtt ttacttggag ctatacatgt aactatttta tccttgtact ctgggcataa
    33961 tgactgtaaa ggagtatgtt taaatccttt tctaattaaa tcagaatgta tctcatcagc
    34021 tattatccat aggtcatatt ttttacatat ttctacaacc ttttttaatt catcttctct
    34081 ccaaaccctt ccaacagggt tgtgaggact acaaagtatc agtactttat tgttgctaca
    34141 cttagctttt tcttctaaat catcataatc catcctataa gtaccattct catatattag
    34201 aggattgttt acaacgtccc tattattgct ctttatctta gcttcaaatg ggtaatacac
    34261 tggtttttgg actattacac cttcattaga attagttaat attcttacta aaatagcaac
    34321 agcaggtaca acaccaggac taaaaataat atcttttcta tttatttccc agttaaatct
    34381 ccttttatac caatcacaaa ctgcattaaa gtatttatca ctatcatttg aactatatcc
    34441 aaatatttta tgttcaactt ttttacgtaa tgcttctatt attggttctg cacattcaaa
    34501 atccatatca gcaatccaaa aaggcaaacc atctttttgt gcttttgttg aacaattagt
    34561 tctaaaatcc cattttacac aatctgttcc tactctatct attactctgt caaaattata
    34621 cttactcatt ttttccctcc taagaataaa atttttataa ttgttaagtc tattactaga
    34681 tgcactttgg tattatttta caattcttaa tattataata atatcacttt tttgtccaat
    34741 agatttaaaa tataattact tttccatcat attttttcaa tattccaaaa tcagtttcat
    34801 tatatcaaaa taagattgct catattaatt attttctaat taacataagc aatctttttc
    34861 tataacttct ataattataa ttttaaatgt agaattatta gtttggatgt tatcccaatc
    34921 tcttttcaac caatctacac catatatcta caccatattc taaagctttc gtatcaaatg
    34981 ttacatttgg attatgaaat cctggtgtag catcagctcc taatccaata tatgtagttt
    35041 tacattttag tttttgtgta taataatgga agtcttctcc accagaattt ttaaatatac
    35101 ctatagatgc atcagctccc aatacatctt ttatagtttc ttcagccaag tttacaagtt
    35161 cttcatcaaa ttctgctgct ggtactccat ctttggaaat tagtacagct tctgcattta
    35221 tagaacttac aacagttttt acagcatgta ttgctttttc ttttaattct tctattatat
    35281 cattactttc tgcccttata tctaaagcca taaatgcttc atctggtata ttattataag
    35341 tttctccctt aactgatata tttgttactt ttatagaatt tggtatagct ggatttactc
    35401 ttattgcatt tattgcattc actgctaaac agacagcatc tactgcattt actcctaaat
    35461 gtggtttaga accatgacaa gtgatacctt taatcgtaaa ttccattata cagctagcac
    35521 aatggtacat tgcaggtatc gcctttccca acctacaatc ttgtccaggt cttaaatgca
    35581 tccctaccat ttcttctaca tcatccaaca aaccagaatc aattactctt aatgctccat
    35641 ataatttttc ttcacctggt tgaaaaagta tttttaactt acctcttttt actaaatttc
    35701 tctcttttat taactttgca gctgttaaaa gcatagaagt atgaccatca tgaccacaag
    35761 catgtatcat tttcttttct ccatctatga taaattccaa tgcatccata tctgctctta
    35821 tagcaaatac tggtccttct tttttagaat ctatagttac tataatacca gttcctgcaa
    35881 ctcctttatt aatattttca aaccccattt tttcaagctc ttttaaaaca aactctgatg
    35941 tcttatattc ttcaaatcct ttttctggta tagtgtgtag ataatcatac actttatgta
    36001 catactcaat ataatctttg ttagttccca taatctagct cctccttaaa atttaaactt
    36061 tccatttata ttaaattttt attataatta cctatactac gaaataattc attataagca
    36121 ttgcaataca accatttaaa gttgctataa caaacatatg tggataatat ttagttttta
    36181 actcagctgt tcctaaaagt ctccccataa attgtatctg cgctcctgaa agcaataaca
    36241 ttggcaatag aattgcaaca tgtgcttgac ttatttgacc tgctgtaaat aaacctgcga
    36301 ttactccaga tgctcctccc attgatataa atattgtcat tatagctgtt gctgcaacac
    36361 ctggaagtcc aaagatactc attacaggtg taaatattgt ccctattaca tccaaaagac
    36421 ctgttaagtt gaacacagaa attatagcaa aaccgaagat gacatttgga gccattgaat
    36481 ttgtacttat ttggaatcca tttctagctc ctccaataaa cgcatctatc acatttcctt
    36541 ttttaacttt tacttctttt tttgctacct cactcatgtt aagcttcccc cttgtcaaat
    36601 ttatttaaat ataatctaag taagttcgcc ccaacaaact tcataattaa tattaaagca
    36661 agtggtataa atattggtac atctcctata aatggaaata gtgctgctcc agaagcaaag
    36721 aaattcgtaa ttgcagacgc acctgaaaat tgaaatgcac aaaatacagc tttttgtttt
    36781 tcatctatat acccatcatc tttaagttct ttagtcatag atgcagctgc atctgtagac
    36841 tgtaaactag ctatcaaagt tagtcctgca aatccaggta accctaataa tggctttaat
    36901 agtggactaa gaagcttttg agctgccttc attccatcta agtggtctat aactctaact
    36961 actccaagtg caaacataac tgatggtatt aatgtcaatg catatagcca tccatctcga
    37021 gggccagtac ctccatcacc tctaaagttt gcagctaatg tacctacacc atcattaatt
    37081 gttcccaatg ttccaaaaga acctaataca ttgttaaaat ccaatacttt taatggtcct
    37141 ggtgcatctt taaatattcc tgaaaaaaat aatgctccca atattaatgc tacataccca
    37201 taccatttta cctcaacttt ttctgttgtt gattctaatt tactcatata aattctccct
    37261 cctgtttaaa tgaattttat tagtatactt gtttaataag caaaacttat gccaatacct
    37321 taatgataaa gcctttgtat ttattttttt attgtaaatt tattccagtg attaaatagc
    37381 ttataattac ttacttttca agtttaaaaa tataata
//
LOCUS       DAMJYC010000032        37372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJYC010000032 DAMJYC010000000
VERSION     DAMJYC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.033"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            204..1754
                     /locus_tag="PC472_001766"
     CDS             204..1754
                     /locus_tag="PC472_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2727092.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            1780..4482
                     /locus_tag="PC472_001767"
     CDS             1780..4482
                     /locus_tag="PC472_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2727093.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIG
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVQVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(4988..5653)
                     /locus_tag="PC472_001768"
     CDS             complement(4988..5653)
                     /locus_tag="PC472_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727094.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            5997..7427
                     /locus_tag="PC472_001769"
     CDS             5997..7427
                     /locus_tag="PC472_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2727095.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            7795..8373
                     /locus_tag="PC472_001770"
     CDS             7795..8373
                     /locus_tag="PC472_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2727096.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            8403..8549
                     /locus_tag="PC472_001771"
     CDS             8403..8549
                     /locus_tag="PC472_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2727097.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            8854..10233
                     /gene="scfB"
                     /locus_tag="PC472_001772"
     CDS             8854..10233
                     /gene="scfB"
                     /locus_tag="PC472_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2727098.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            10262..10759
                     /locus_tag="PC472_001773"
     CDS             10262..10759
                     /locus_tag="PC472_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2727099.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            11057..12721
                     /locus_tag="PC472_001774"
     CDS             11057..12721
                     /locus_tag="PC472_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2727100.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            12734..15184
                     /gene="recJ"
                     /locus_tag="PC472_001775"
     CDS             12734..15184
                     /gene="recJ"
                     /locus_tag="PC472_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2727101.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     NIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDVSLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            15197..15709
                     /locus_tag="PC472_001776"
     CDS             15197..15709
                     /locus_tag="PC472_001776"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2727102.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            15783..17990
                     /locus_tag="PC472_001777"
     CDS             15783..17990
                     /locus_tag="PC472_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2727103.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            18015..18464
                     /locus_tag="PC472_001778"
     CDS             18015..18464
                     /locus_tag="PC472_001778"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2727104.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            18479..19093
                     /locus_tag="PC472_001779"
     CDS             18479..19093
                     /locus_tag="PC472_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2727105.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            19184..20740
                     /locus_tag="PC472_001780"
     CDS             19184..20740
                     /locus_tag="PC472_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2727106.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNADNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            20730..21992
                     /locus_tag="PC472_001781"
     CDS             20730..21992
                     /locus_tag="PC472_001781"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2727107.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            22066..23853
                     /gene="aspS"
                     /locus_tag="PC472_001782"
     CDS             22066..23853
                     /gene="aspS"
                     /locus_tag="PC472_001782"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2727108.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETSPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            24312..25544
                     /locus_tag="PC472_001783"
     CDS             24312..25544
                     /locus_tag="PC472_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2727109.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            25558..26364
                     /locus_tag="PC472_001784"
     CDS             25558..26364
                     /locus_tag="PC472_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2727110.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(26476..27345)
                     /locus_tag="PC472_001785"
     CDS             complement(26476..27345)
                     /locus_tag="PC472_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2727111.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            27717..29144
                     /locus_tag="PC472_001786"
     CDS             27717..29144
                     /locus_tag="PC472_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2727112.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(29221..29490)
                     /locus_tag="PC472_001787"
     CDS             complement(29221..29490)
                     /locus_tag="PC472_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727113.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(29493..30935)
                     /gene="nhaC"
                     /locus_tag="PC472_001788"
     CDS             complement(29493..30935)
                     /gene="nhaC"
                     /locus_tag="PC472_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2727114.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(30960..32126)
                     /locus_tag="PC472_001789"
     CDS             complement(30960..32126)
                     /locus_tag="PC472_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2727115.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVVLPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(32351..34471)
                     /locus_tag="PC472_001790"
     CDS             complement(32351..34471)
                     /locus_tag="PC472_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2727116.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKKSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            34704..35105
                     /locus_tag="PC472_001791"
     CDS             34704..35105
                     /locus_tag="PC472_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727117.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            35148..35441
                     /locus_tag="PC472_001792"
     CDS             35148..35441
                     /locus_tag="PC472_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2727118.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            35456..35896
                     /locus_tag="PC472_001793"
     CDS             35456..35896
                     /locus_tag="PC472_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2727119.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(35988..36461)
                     /locus_tag="PC472_001794"
     CDS             complement(35988..36461)
                     /locus_tag="PC472_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2727120.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(36461..37249)
                     /locus_tag="PC472_001795"
     CDS             complement(36461..37249)
                     /locus_tag="PC472_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2727121.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
BASE COUNT        14525 a         4162 c         6321 g        12364 t
ORIGIN      
        1 aaatttgtaa aatgaataga tataattact aaatatgtga tagtattggc tataatagga
       61 tataaaaaat aatattgtaa taaaaagttt atatttagta atgaaaattt aacgttttac
      121 ttgaaattat gtgttttgta atgtattatt aaggttctaa taggaacatt ttcatcaatt
      181 tttctatagt gggggtagga attatgaggt acaaaaaaag aaatatagat aagtcaagtg
      241 aactttcatt tcaatctcta gcaaaatatt ttataacttt ctatatagta attatattaa
      301 ttattctttc tatgttttgt ataaatacaa tatgggttat gaaaaaagat aatcaagatt
      361 tattatctac aacatcaaat cgtattgaag atagggtagg agaaacaatt aagctattga
      421 actctttgag taaagataat tacataatag ataatagtgt gaatggcata gataaaagta
      481 aagaactttc aatgtatagt gatgaatttg ggtatatgat gatatgtttt gttgataaga
      541 atataattgt gtgggatgaa gaaggtggta atattagtct agctggtcgt gattatatgc
      601 agaaactttt tgcaacaaaa aaatatcaag ttacagatag ttttgcagca ggggctgatg
      661 gaaaaacttt aaactacact attgcatcac ctataataaa gaatggaaat gtagatggtt
      721 gtgtatttgc agcaatttac tttgatgaga taaaggatat actcaaacaa ggaaaaggtc
      781 aacagcaaca gaatttcata ttatttggaa gtgaaaatca aataatgaat ttaacaagtg
      841 atgaaactgc atatggagag aagtatatga agttatcgca gaaaacaaaa tatcttggaa
      901 ccaatgcaaa aaaagtagaa aaggatatgt ttaataataa atcaggtgat tattgggctt
      961 ttgaaaatgg aaaattatac tttatgtcct ataaaccaat taagaacaca aattggagtt
     1021 tattttgtaa agttaatttc ttagatattt ttgcattaaa tttaattcct atgggagcta
     1081 ttctatctgt tataacttta gtatttatta ttttgatttg tatggagaaa agcttcatga
     1141 aaaaacgaat ggaagttatt gatatgcttt taggctctgt tcaagaatta gaaaaacgta
     1201 tatatcaaac agaaagacct agtgaagtag actttaaaga aattattcag ctcacaagta
     1261 aaggtctgac tgatggatta actggtgttg taacacgtac tgtatttttt gaccaaattt
     1321 ataatcgtgt tgaaatggag gaaaatgata aattatctgt aatttcattt gttgatttag
     1381 atgatttgaa atgtattaac gacttatatg gacatgaaat tggggatatg gttttgaaaa
     1441 atgttggata tgtacttcgt gaatatgaga taaaatataa tggtttaatt gggcgctatg
     1501 gtggagatga atttgttatg ttgttatctg gttttacatc gagagaagag gtggaggatt
     1561 tattggatga gattgctatt cgcttacata ctgaagtact aacagatgat ggtatcattc
     1621 aaactcattg tagtgttgga gctgtagtat gtagtgtaaa agacaataaa gatatagata
     1681 atttattaaa acaggctgat gaagctttat actatgttaa acgtaatgga aaaggtaatt
     1741 atcatttagt ttaatgtatg tggaaggagt ttatatcata tgaaaaatgt atctaaaaaa
     1801 ttttttgtat gtactattat tgtattaata acatttttat gttttagtaa atttgctatt
     1861 tatgtttttg acaaacaaga aaagaaagaa acaaaagtag tagaaaacat gttaaatgtc
     1921 tacaagaata acattatttt aaatctgact gaaaaattta attatagcaa agacttgaca
     1981 tcagttttag tttcaaacaa ttattctata gataaattaa aacctattgc agatgaaatt
     2041 cttaaaaaca attcttgcat atatatttca gttgtagaaa aatataatgt agctgcattt
     2101 ttttctaatt ctgtcaaaaa agatattatc aatactccaa tacaggaact gaattattct
     2161 tattcattag caaattctat caaaggatta gtcgtagaag gtccttctaa agaccagttt
     2221 tcaaatggag atgtgttttt aattactaat cctatttata aaaatggaga gtactgggga
     2281 gaaattacag tagctttaga tgccaaaaaa gttgtagatt cttttaactt aaataaatta
     2341 aaagaatctg gatttgagta tgaactttgg cgtgtacacc tagataatgg ttcaaaatct
     2401 acaatagaaa tttctactaa agattttaat ttttccaaat cacatgaaat taagtttaag
     2461 cttccgactg tttggactct tagcattgtg tacaaaaatg gatggaataa taagaatata
     2521 gattttattg tatttgttat attgttaatt atttccatat taataggtgt gatgacattt
     2581 atttacattg aaaagaagtc gattggtaag gaattaaatc aacagagtct taaaaattta
     2641 gaaactggta ttttgaatag agaaggtttt ttaattaagt tacaagagca tctgaataga
     2701 aaaaataatg aaatagtatt gctgtatata tgtattacta attatagtaa atttgctcca
     2761 gttatgagct ttgaaaaaag acaagattat cttacacata tagtaaaaaa tctagataat
     2821 tacataaaaa gttctcattt tattgctcgt ataagtgagg aaggttttgt tctagcttta
     2881 gaagaaaatt gtgataaaaa tgaattgctt aacattgaaa aagggatttc cttagagctt
     2941 ttacgaaaaa ttaaagtaga aaataaacaa gtatttttaa cgattggtta tggctcatct
     3001 aggtgtgttg atgatagaaa agatgcagaa tctctgttac tttcagctat tgaagattat
     3061 gagagaagat ttccagcaat gtctcctatt catgatttta ctaaaaagtg tgaagatatg
     3121 cttgatgaaa agaaacagat tgtatttggt gaatatccag atttagaaat aaacaagttg
     3181 tctaaagtaa tatacaaata ttataaaaaa gtacagcgta ttatgtataa agattataat
     3241 attgatattg gaaatagacg agggtatatg cgtgatgttc aagtattaat tgattataat
     3301 tctgagagaa tgttatactt atttttaata gacatatgca agtttggaaa atttaatgaa
     3361 ctgttttcta ttgaaatagg agatgttatt ttaaaagaag ttagtaacaa attaaaaaca
     3421 attttttcta attatctata tcgtattaat ggagatgtat ttttaggcat ttcaatagat
     3481 aaagaatctt acttagatac tttacataag gttaatgaag tatttaatag ttctttaagt
     3541 gtagaagatg tactttttga tgtaaaatgt aatattggta tttgtcaata tccatatcat
     3601 ggtgaaaatc catatgacct tctggaaaag gtacaaattg cattggaata tgcaaaaaag
     3661 gatgtcaaaa gtaatgttaa agtttatgat aaggaattga cagaagagat ttaccatgaa
     3721 aataatatta ttaaggattt aaaaaaaggt ctggaagagc aaacactaga agtttggtat
     3781 cagccaattt ttaatgttag taaaaactgt tatataaaag ctgaggcact aattagattg
     3841 agaaataaag agggcggtta ttattctgct tatgaagtta ttacttatgc agaaaagcat
     3901 ggacttatta aggatgtagg tctatatgta ctaaaaaagg cttgtgatgt aactacaaaa
     3961 ataaaagata aaaatataga ttttgaacaa atgcaattaa atttatctgt tcagcagatt
     4021 gtacataagc agtgtgcaaa tgacatatta tcatgtattg agaattgtgg tttagataaa
     4081 aagtatattt gtgttgaagt tacagaaaca gtacttatgg aaacttttga aattgtaaat
     4141 gttactcttg ataaactaaa aaaagaaggc attagcatag ctttagatga gtttggttct
     4201 ggatttagta gtataaatta tctggcaaat ttacctattg aaaatttaaa aatagataag
     4261 gaattagtag aacaaattac ctattctaaa aaacaatttg aattattaaa aactatagtt
     4321 caaattgcta agattaatgg catggatatt gtagcagaag gtgttgaaac aaaagaaact
     4381 ctggatttag ttgtaacttc aggtgtgaat ttgattcaag gtcattattt ttcagaacct
     4441 ttaagtgaag ataaatttat tgaacttatg attgaagaat aaaggttaat aaaataaaga
     4501 tttaaggtat tgaaaaataa ttaaaagtat ttaataatag aattagggaa gaataagttt
     4561 ataattaaaa ataattgatt agtagttaat aaaataaatg tgatatccaa ataaatccta
     4621 aatctaattt gagttttaat atagatagtc taaagtttga ttattaaaga tttaattgta
     4681 agttgttaca gttgatgata ttgattagag attaaagttt agagtagaaa gttaccttaa
     4741 atttaaaaat gcaagataac ccaatctaaa aattataatc actcgatagg tttaaggatt
     4801 tatttgttta ttataaatag gagggtgtct caaaatgaac tttttagttt atgaggcact
     4861 ctcttttata tagaattaaa tatattttat tatttcaaca atgaaaaaga agcttatctc
     4921 tattttgaga cagcctcttt gtatactatt attaatttaa tcaataacat aatttaaaat
     4981 tatattatta gttaagattt cctgctttat tagtaaataa ttctttaata gcattgtttc
     5041 tggaaactaa atcgttatca gagttgtttg tttttttcat aatcactgaa taacgttttt
     5101 tatttataaa aaatattaaa atcttatcat catcaaaatt gtattcttta tatctgtcaa
     5161 tagactcata acctagattt agaatgtttt tgtcttttat actatctaca tctaaaatat
     5221 atgatgggtc agcctttagt aatatattaa ttaaacattt ttcttcacca gcacagaata
     5281 actccatctt acttgatata gatttagatt gctctgaagt tatttttgca ggaataaggt
     5341 tgtcattatc atctcttaga agataaccat gtgtgtcttg ttgatatact aaattaacct
     5401 ttttcttagg aacactaatc attttatcta aatgtacata atgatgattt tcaacaaaac
     5461 cttcatttaa tatttcaaca aagttattaa ttttacctag gcaaaaagat tctatagatt
     5521 ctttacagaa tttatttgta aatggtatta tgtttacgtt ttcctcgtct gtattccatg
     5581 ctatagcata atccaacgaa tatacttctt cctctatatc tgcaaaatta tattgtacta
     5641 tgtttccttt caaaattact cgacctccat cttataatat atttaatata ctttataaaa
     5701 cttatattat atttaaataa tatcataaat tggataaata tgatatagta aataagaaaa
     5761 aatttgaaaa aaatcactat aaatgagtaa taaatataaa tagtgtatac taatgggatg
     5821 gaaaatgtcc actattaact ttattacccc acatatataa gataaaatta ttaatataaa
     5881 atataatcta tcgacaatga atagactttt aaataaactt aatataaaat taattaaata
     5941 agtgcttaaa aacgatttac tttaatgata gaatcatatt aaaggaggat aattttatgg
     6001 atataaagaa taaaattaaa tctatatttt taccagaaaa aaatgactat ttagatgatg
     6061 aagaatttga gtatgatata gaaaataatg aagaagattt aaaggaagat aacaaagtac
     6121 atttaaatga tttaagcaga gtaaaatatg attacagaga gttaaaagac atagaaaacc
     6181 aaacagaaga gatgtgtctg ctagcagtaa agcaggatgg tactataatt gagtttgtaa
     6241 aggacaagac ttataaagta tgtatggaag cagtaaaaca gacatataaa tctcttaagt
     6301 atataacaaa tcaaaatgag gatatttgta tagaagcagt aaaacagaac tacagagctt
     6361 tgtactacat aaataataag acagaaaatg tactaattga ggctataaaa aatgcatcaa
     6421 cttatgatgt catggaagta tttaaatttg tggaagagca aacagaagat gtatgtctag
     6481 catttataga aagggcatct aaaaatgatg ttgcagagat attaaaagga ataaaagagc
     6541 aaactccagc catatgttta gaagctgtaa aaaaagatgg aaaatcttta gcttatgtaa
     6601 aggaacaaag taatagtata tgtttagaag ctgtaaaaga aaactacagt gcactatctt
     6661 gtgtaaaaga gcaaactgaa gaaatatgta tagaagcagt aaaacagaat gactttgcat
     6721 tgtattatgt aaatgaacag acagaaaaaa tatgtatgga agcagtaaaa agaagttata
     6781 tggctctaca gtatgtaaat aagcaaacag aagagatttg tttggaagca gtaagaattg
     6841 atggaagagc attacagtac gtaaaagagc aaacagaaga aatttgcttg gagtcagtta
     6901 gacaaaatgg aaaagtactg caatatgtaa aaaaacagac tgaaaatata tgtatagaag
     6961 cagtaagagg ttcatttgaa gaacttgaaa taaaagaaat attaagttat gtaaagatac
     7021 caacagaaag aatatttgta gaagcagtaa agcaaaatgg aaaaattctt aaatatgtag
     7081 aaaatcagac agaattaata tgtttagagg cagtaagaga aaactataat gctttagctt
     7141 atgtaaagga acaaacagaa aagatttgtt tagaagcagt taatcaaagt tatgaagcgc
     7201 ttaaatatgt gaaagaacaa acagaagaag tttgtctaaa agcagtaaaa caagattata
     7261 gaatgttgaa atatgtaaat aatcaaacag aaaaaatatg tttagaagca gtaaagcaaa
     7321 attatagagc tttagaattt gttgataatc aaacagaaaa agtttgtctt gaagcagtaa
     7381 aacagaatag aaaagcacta cagtatgtaa aacaaaaaca aagttaaaag gctaaaatat
     7441 atagaaaaca tatgcaaata aataaaattt gatattttgt aataaataaa atgggtatct
     7501 caaaaggaac tttttagttt gtgaggtact ctttttatat gaaatgaaat atatttttag
     7561 ccttttaaca atggggaaaa ggtttatttc tattttgaca taggcttttt atgcatataa
     7621 agcttgataa aattgtttta ttataattgt tttgttatat aaataataag aagaaaatta
     7681 tataggtctt tttttgacca gttttacatt tttaacaact agttttgtaa agtatattga
     7741 attttctcaa aaaagaatgt actctatata tgtaaaattt ttttggaggt aaaaatgttt
     7801 aaaagatatg ctgaaaaaat gacatctgtt ttaatttgta ataatatgat agataataat
     7861 gaatctaaag tctattctta tggatttgaa atattaatag ctttcatagt taatataacc
     7921 accatgctgt ttataggttt tttatttggt aaatttacat atgtattatt ttttttaatg
     7981 tgttattgtc caataagaca attttcagga gggtatcatg cagacaatta ttttagatgc
     8041 ttattaacct ttatttttat aattttatct acaatcttaa ttatagaaaa tataaatata
     8101 gacttattta aaaatattat tatgattatt gcaagtgtta gttgggttgg aatttgtgtt
     8161 ctgtgcccaa tagaacatag gagtaaccct ataagtgata gggaaaaatt ggtttataag
     8221 aaaactgcta tatttatatc tacagtagtg ttattgataa caattttaag tctttctttg
     8281 agtatatttg tagattattt cacatatagc gcatttgcca tgttttggat ttttgtgatg
     8341 ttagtattag gtaagttaaa agctaaagta taagtaaaat atgtatgagg gagagttaag
     8401 ttatgaaaaa gtttattgta agatttatga agtttgctag ctcattggca cttagcactg
     8461 ctattttgtc agctaatagt acctgtcctt ggattattca tcagcctaaa gtacccaaag
     8521 aaatcagcaa tctaaagaag acaaattaaa tacaagcact tatttataca aaattatcag
     8581 gaaaaaagaa attattatat tttaataaga ttaaaacaat ataaaatcag atacaaaatt
     8641 tattttattt attttatagg agcctaatac ctatttggta atatattagt gcgccaattt
     8701 agggggatat agtgtattta atgaaatgac ttacggagag taagtcattt ttgtattgta
     8761 tgtattttat gtaaacttat gatataatga agggtaaagt ttaattaaaa taataaaaaa
     8821 ctttagtata gatagtaatt taggaggtag ggtatgagta tgatacataa attttctatg
     8881 aatggatata atatcgtttt agatgtgaat ggtggagctg ttcatgtgtt agatgatgtt
     8941 gcatacgatt tgttagattt ttataaagaa aaaagtaaag aagaaatatt agagatatta
     9001 aaatcaaaat atcaagaaga aaaaataaat gaagcatatg aagaaatatt aaatttggaa
     9061 aaagagggtc ttttatatac agaagataca tatcaatatc atccaagctt tgtacataga
     9121 gagcctgtag taaaagcttt atgtttaaat gtagctcatg attgtaactt aaaatgtaaa
     9181 tactgttttg cagctcaagg tgattttgga ggagaaaaag aacttatgag ttttgaagtt
     9241 ggtaaggcag caattgacta tttgattgct aattcaggaa gtagaaagaa cctagagata
     9301 gatttctttg gtggagagcc tttgatgaac tttgaggttg taaaacaact ggttgactat
     9361 ggaagaagtg tagaaaaaga ttataataaa aatataagat ttacaataac tacaaatggt
     9421 gtactgctaa atgatgagat tatagattat ataaatgaaa atatgcataa tgtagtttta
     9481 agtttagatg gaagaaaaga agttaatgat aatatgagac caactttaaa tgataaagga
     9541 agttatgata ttacattacc acgatttaag aaacttgtgg aaaaaagagc aaaagataag
     9601 tattattata taagaggtac ttttacaaga gataatttag atttttcaaa agatgttatg
     9661 cattttgcag atttaggatt taaattaacc tctgtagagc cagttgttgg agatgagagt
     9721 aatccatatg cacttagaga agaagattta cctaagatat ttgaagagta cgaaaaattt
     9781 gctgtagagt atgcagatag acaattacaa ggagatggat ttaaattttt ccattttatg
     9841 atagacctaa atcaaggtcc atgtgtaata aagagaataa ctggatgtgg agctggaaat
     9901 gagtatcttt ctgtaactcc aaatggagat atatatccat gtcatcaatt tgttggaaat
     9961 gaagaattta aaatggcaaa tatatttgat gaagaaatag tacttccaga aaatttaaaa
    10021 aatatgttta gagaagctca tgtatatact aaagaagagt gtaaacaatg ttggaataag
    10081 ttttactgtt caggtggatg tcatgcaaat gcgataaact ttaataatga catatcaaaa
    10141 ccatatgaat taggatgtga aatgcaaaga aaacgtacag agtgctctat aatgatacaa
    10201 gctaagttaa tgttagaagg agcaaccaac taaatttata ttttaaagga gttgtttata
    10261 tatgattaga gattatttag aagataaacc tttaattgac gaaagtgtat ttgtggcaaa
    10321 atcagcagat gtaattggaa atgttaaaat aggcaaagat tcaagtatat ggtacaatgc
    10381 agtagttagg ggtgatgaag gaccaataac tattggagaa aacactaata tacaagattg
    10441 ttctatagtg catggagata cagaaactat aattggtaac aatgtaacag ttgggcatag
    10501 gtctatagtt catggttgta aaattagtga taatgtgtta attggtatgg gttcaataat
    10561 attggataat gctgaaatag gagaatatac tctgatagga gcaggtacac taattacatc
    10621 taataagaag tttccaccag gtgttttgat aatgggttct cctggtaagg ttgttagaga
    10681 gcttacagaa gaagataaaa aatatattga tgaatcttat gaatggtatt tagaggcagc
    10741 acaaaatcaa aaatattagt ttaaagagtg gttgtttcaa aacatattta aatctgtttt
    10801 gaggcaacca ctaatttatt ttattagatt ttgttgatat tttagttttc aaaaagtatt
    10861 tgattaaacc agatataaaa tgtcaaaaat aaaaattaac tgagttaaat aattttaata
    10921 aaattggtag tatatgtgat aaaattaaat attaaagagg atataggata ttagaatatc
    10981 aattggtgaa ataatgtcat atacgataaa ttaaggtgta agtttttggg tatacctatc
    11041 ttaggagtga aaagatttga gaataagtta tagaaattta aagcgatata gagaaattgg
    11101 atatgtttta ataaagtatg gttttagttt tattgtagaa agactaaata tagaaggaat
    11161 tgcctataaa ataccactat ttgacccacc agaagaaata aaaaatatga caactggtga
    11221 gagaatgaaa agagttttag aagaactagg acctacatat ataaaaatag gacagatatt
    11281 gagtacaaga aaagacttgt tagaccagga tataattgat gaaatatcaa aactcagaga
    11341 tgatgtagag aaatttgatt caaatatagc tattgatata tttaaagaag aagtgggttt
    11401 aagtatagaa gaaatattct tagaatttaa agaagaacca atagcagcag cttcaattgg
    11461 tcaagtttat gaaggtgtac ttaaaacggg agaagaagta atcgtaaaaa ttcaaagacc
    11521 aaatatagag aaaataataa aatctgattt agagattctt agaactattg caaatacttt
    11581 aaaagattta aaaaaggatt ttaatttaga tttagtacag atgattgaag aatttcaaac
    11641 tcaattaatg agggaacttg attatacatt tgaagctata aatgcaacta aattctctag
    11701 gatatttaaa aatagtgatg aagtatatat acctaaagta tactcagaat ataatactaa
    11761 gaaaatttta gtaatggaga aagttaatgg tacaaagtta agtgatgtag aaaaaataag
    11821 gagactaggt tataacacaa aaactattgt tgaaatagga gttaggtcat tttttacaca
    11881 ggtattatct catggatttt ttcatgcaga cccacatcca ggtaatatat tcgtagttgc
    11941 taaaaataag atagcatata tagattttgg aatgattgga attattgata ataaaacttt
    12001 aaaccaatta aatgaaattg cattagcagg tgtagaaaag aatgttgaca agataatata
    12061 tctattgata gaaatggatg cattaaatgg agaggctgat attaagggac ttagacagga
    12121 tttactttat ctaatacatt attactatga tatttctata gaaaaaataa atgtaacaga
    12181 tatattaaat gaactattta ggttttttag acagtataaa atagtcatgc ctgcacaatt
    12241 tgtaactctt gcaaagacag ttataacatt agaaggaaca agtagaactt taaacacaga
    12301 tttttctttt ggttctatgg gaaaagagtt tatgaaacat cattataaga gtaaatttaa
    12361 ccctaaaaat gttgttttaa gttcaagaca aaatgtagaa gaaatattac tggacataaa
    12421 aactattcca aagcaattaa aagcaatttt aaaaaatata gaaagaaata atataaaaat
    12481 gcaaattgaa gatgttaaaa tgacgaggtt agaaaattgt ataatagagc taacatctca
    12541 aatatctttg agtttagtgt tggcatctat tatagtaggt tcttctctta taattgcatc
    12601 tccaaatata gaaaataata tatggataaa atttactgca atagcgggtt tctttatatc
    12661 ctttataata ggcctttgct tagttataag aagtattagg tcaaaataca aaaaagatta
    12721 gggggaatta aatttgaaaa aatggatatt gaagtataaa ggtaagatag aggaaagtga
    12781 atttagcaaa aaacttaata tttccccgga aatctgccaa attttaaaaa atagagggat
    12841 atgtactgaa aaagattcag aaatatttat gaatccatct ttagattatt taagagaccc
    12901 ttttttaatg aaagatatgc aaaaagctgt tgatagaata aagttagcag ttgagaagaa
    12961 cgaaaaaatt tggatttacg gagattatga tgttgatggt gtatcatcta catctatctt
    13021 gtgcttatat tttaagagta taggttacaa tgttgattac tatattccta atagattaga
    13081 agaagggtat ggtataaatg aagatgctat taagcttata agctctaggg gatgtgactt
    13141 gattataagt gttgactgtg gaattacatc agtgtctgag gtcaatacag cgaatgattt
    13201 gggcatagat gttataatta cagaccatca tgaatgtcaa agtgaaattc catcagcctt
    13261 tgcagtaata aatccaaaac aagaagattg taattatcca tttgattcac tatgtggatg
    13321 tggtgtagca tttaaaatga ttcaagcctt gactccaaaa gaagaattta aaactagtat
    13381 gtataattat ttggaaatag taacactagc aactatttgt gatattgttc cacttataga
    13441 tgaaaataga ataattgtaa aaaatggttt aaaatcaatg aaagaaggca aaaatattgg
    13501 attaagggag cttataaaag tttgtggtgt agaaagtgat aagataggtt cttctcatat
    13561 aggttttgca ataggtccta ggataaatgc ttcaggtcga ttgggatatt cttacttagg
    13621 agtagagcta tttactactc aatcacaaga agaggcagta gaaatagcta gtatattgga
    13681 agaaaaaaat aatgaaagac agatgataga agctaagatg tatcatgaag ctgaagaaat
    13741 gctaaagtct aatagcagat acaatgatga caaggtgttg gttttagcta aagagggttg
    13801 gcaacatgga ataataggaa ttgtagcatc aaagttaact gaaaaatact acaaaccaac
    13861 aattcttcta ggtatagaaa atggggaggc tactggttca gcgaggtcaa taaaaggatt
    13921 taatatattt gaagctctca taaagtgcaa agacttgatg actaagtttg gtggtcatga
    13981 acaagcagca gggttatcct tggattcaga caatgtagaa attttagcaa atgaaataaa
    14041 taaatttgca gattataatt taactgaaga tgatatgata gagaatgtaa atgttgagtt
    14101 tgaattacaa gaaaatgtaa taaatttaaa tcttgtagaa gaattacata agttagaacc
    14161 ctttggatta aataatccta atcctagatt tatagttaga aattatattt taaaagattt
    14221 gaaagttata ggtaagaatc aacaacatct gaaattaaac atagaaaaag agaaaagcta
    14281 tgaatgtata ggatttaata tgagtcattt aaaatcaatg tataaagtag gagacaaggt
    14341 agatgtacta ttccaattag atgaaaataa ctatatggga aataggaaag tacaattttt
    14401 acttaaagat atacgattgg ctcgacctaa aagtgcaagt aatgacaaac tttcattgaa
    14461 attaatgtca aaaataatac caaaagatac tcaaagcctg tataatatat ctgtgtctga
    14521 ttttgaatta tttgatggaa atacagatat aaatatattt gattattttg aaaaagatac
    14581 actaataata tctaattcta taaatggatt ttatagagca atgtcagatg tatcacttat
    14641 agatttggat tttaacataa attacaatat tattgaagat gatagcaaaa atactgataa
    14701 attagagctt atattttctc ctaatattga taaaatagac ttaaaaagat ataataatat
    14761 tattctttat gattatttat acaataaagg cgaatattcg tatatatatg agaataaaag
    14821 agaagaaagt gaaattataa aatattataa taaaacagac ctattatatt taaagaatgt
    14881 agttagtaat atagttccat caagagatga atttataaca atttataaac aagcactcat
    14941 aaaaaaagaa attgatttgg atatggtaaa tataagggaa acttttaatg taattccatt
    15001 aaagtttttt acgatattaa atgtatttag agaattaaat ttacttgact ttaatttaaa
    15061 ttatgaaaaa aattctgtac tcataagaat attgccaaaa ccacagaaaa aattagactt
    15121 aaatgagagt ttaatactca ataatctaaa aaatttagaa aaacaataca atagcagtta
    15181 ttaggaggcg tttcccatgg atttaaaaaa ctttataagg aatattgatg atttcccaaa
    15241 gccaggcata gattttaaag atgtgacaac tctatttaaa gatggagatg catttaaata
    15301 tgcagtggat tctatagttg aagaattgaa agataaagat gtagatttag taataggacc
    15361 agaagcaaga ggatttttga tgggtacacc tgtagcatat gctttaggag taggatttgt
    15421 tccaattaga aaacctggaa agttaccagg tgaagttgag agttatgagt atggattaga
    15481 gtatggtaca gatactttag agatacataa ggatgcaatt aaaaagggtc aaaaagtagc
    15541 aatagtagat gatttattgg ctactggtgg aactatggaa gccgctgcca agcttgtaga
    15601 aaaattaggt ggagaagtag tttctatgca atttttgatt gagctaaaat tcttaaatgg
    15661 gagagaaaag ttgtctaact atgatgttaa ttctcttata aaatactagt acttttataa
    15721 tagttggtat aacaccaact attttcatat atattaaaat tttatacaaa ataggtggtt
    15781 atatgcatga taaagaatta caagaaataa ttgaaaaaat aaaagtttat gcacctaatg
    15841 caaattttga aatggttgaa aaagcctata atcttgcaaa aaaagcgcat gaaggacaat
    15901 atagaaaatc tggggaacca tactttattc atccattagc agttgcaaat attttaatag
    15961 agatggaatt agatattgaa actataacag caggtttact tcatgatgtt gtagaagata
    16021 cagaatatac ctatgaagac ataaaaaaag aatttggcaa agaagtagcg gatttggttg
    16081 atggagtaac taaacttggt caaataaaat atcgttcgaa agaagaaact caatctgaaa
    16141 atttaagaaa aatgtttttg gcaatggcga aggatataag ggttatactt ataaaacttg
    16201 ctgatagatt gcacaacatg agaacattaa aatacatgcc tccagaaaaa gcaaagtata
    16261 aagcaaaaga gacacttgaa atttatggtg gtatagctca tagattagga atttccaaga
    16321 taaagtggga actagaagac agagcattaa gatttatgga cccagaaggg tattatgatt
    16381 tagttagtag agtatcaatg aagagaagtc aaagagagga ttatattcaa ggaattgttg
    16441 aacttcttaa agataagttt aaagatttaa gtataccatg tgatgtatat ggtagaccaa
    16501 agcattttta cagtatatat agaaagatgc aaaaaaaaca taagactttt gaagagattt
    16561 ttgatttaac agcagtaagg atattggttg ataatgtaaa ggattgctat gcagtacttg
    16621 gtatagttca tacattatgg agacctattc caggaagatt taaagattat atagcaatgc
    16681 caaaacccaa tatgtatcaa tcattacaca ccactgttgt aggtcctgat ggagaacctt
    16741 tagagataca aataagaact catgagatgc ataatatagc tgaaaatggt atagcagccc
    16801 attggaaata taaagaaggt atctctagca aagagtcaaa ggttgaagaa aaacttcaat
    16861 ggttgagaca gatgatggag tgggaaaaag atttaaaaga cccacaagag tttatggatg
    16921 cacttaaaga agatgtattt aatagtcaag tttatgtatt tacgccaaaa ggagacgtaa
    16981 ttgaattacc agcagaatca acacctatag attttgccta tagggttcat acaaatgtag
    17041 gtaataaatg tgtaggagct aaaattgatg gaagaatagt tcctattgat tataagcttc
    17101 aaaatggtaa tattgtcgag gtacttacat catctaattc taatggacca agtagggatt
    17161 ggcttaatat agtgaagacc ccaaatgcaa aaagtagaat tagacaattt ttcaagaagg
    17221 aaagaagaga agaaaatgtt gaccgtggaa atacaatatt agaaagagaa tttaagaagc
    17281 atgatatacc acaaaaagac cctcttgttg aaaaatatat ggcattagtt gctaagaaat
    17341 ttaatcagcc aagtgttgag gacttaattg ccactgtagg ttatggtggt ataatgtcat
    17401 ctcaagtggt atctaaggta aaagacctat atttaaaaga ggttaaaaaa gctaaaaagg
    17461 aacaacagat acaagaagaa gatttaaata aacacaatat aagtgaaaca gaatacagta
    17521 aaaagagaaa gaaaaacgca tcacaaggaa ttatagtaaa agggcttgac aatatcttag
    17581 taagatttgc taagtgttgt aatcctcttc caggagatga aattgtagga tatattacta
    17641 agggtagggg agtagccata cacagagctg attgcccaaa tgcaaacaat aatggtgatt
    17701 tcttccaaaa tagattggtg gaggtttcat ggagttcagc aggaaaaggt aagtttgaag
    17761 cggaaattca aataaaagct attgatagaa gaggaattgt taatgaaata actcatattg
    17821 ttgcaatgga taaacttagt ttaaacggaa taaatgcaag aaaaggtaag gatagtattg
    17881 taagtataaa cttacttgta gaggttaatg acacagagga attaaaaatt ctaatgaaaa
    17941 aattaaaagc aattcctggt gtagaaagta tttatagaat gacaaattag agttaaaata
    18001 atagggagga aactatgaga gcagtagtgc aaagagtatc ttcatctaaa gttacagtag
    18061 atgaaaacac tataggtcaa ataaataagg ggttactagt attgttagga gtgactcatg
    18121 atgataaatc aagtgatgta gattatatga ttgataaaat attgaatctg agaatttttg
    18181 aagatgaaaa tgacaagatg aatttatctc taatggatat aggtggagag cttttagtag
    18241 tatctcaatt tactttatat ggagattgca gaaaaggaag aagacctgga tttagtaatg
    18301 cagctagacc agaacttgca aataaccttt atgaagaatt tgtaaaaaaa gcaaaagata
    18361 aaggtgtgac agttggtaca ggtcaatttg cagctcatat gatggtagag ttaactaatg
    18421 atggtccagt aacaatacta ttagactcaa gtaaatcttt ttaaggggga aaactttaat
    18481 gattataaaa aaatttgtag atagttattt tggagtaaat acttatgtat taggtgatga
    18541 aaagactaaa aaatgtgcag ttatagaccc aggtggaagt ttagtagaga cattaagttt
    18601 tgtaaaagaa aatgaattaa atattgaata tattatcctt actcatgggc atggagacca
    18661 tataggatat gttaaggata ttaaagaaaa aactggtgct aaagtagttg cacatgttga
    18721 tgaaaaagaa ttattaaatg acaaaaataa aaatctaagt tatacaatga gatgtggagc
    18781 acaagagttt gatgcagata tctatgtaaa tgataaagac aagcttgaac ttggtgaatt
    18841 aaaaatgact ttcttgcata ctccaggaca tactcaagga tgtatgtgta taagagttga
    18901 agatgaaatg ttcacaggag atactttgtt tgctggaagt attggtagaa ctgatttata
    18961 tagtggagac tttaaccaaa tggaaaaatc acttaagaaa ttatgcaaat atgaagatgc
    19021 tgttagagta tatcctggac atggaccaac cagtactcta ggggttgaaa agaagacaaa
    19081 tccttacatg taattttata ttagtattta aaaaagtata aaatatttaa taactatatt
    19141 agtgaggggg ttcttctact gttaaataga aggagttaag ttgatgttgt atgttttttt
    19201 aaaaggacat gattataagt atgaagtagc agaacttata aaattattta ccagtgaatt
    19261 taagtttaaa gactctaatg ctgacaataa tatagagtca aagcatttaa taaatagatt
    19321 gatttatgag aatggtgtat tattctcaag tactgaatac tatgaaaatg gaaacttaaa
    19381 gtacgagtca atacagaata tagaaaatat gaatcttgaa tttttagatg gatttgatag
    19441 tagcaaaacg attgaagaat ttcttaatag cctagatagt gaaagtaaga ggaactttaa
    19501 aaagctgtgt aaagaaaata tcaaaaaaag catgtttgta gtgcttaaac aagtatttaa
    19561 ttcttatgta ccatggggaa ttttgactgg tattagacca gtcaaaattg tccacaattt
    19621 aatggataaa aagctagatg ataatagcat aagaaccatt ctaaaagata attattttat
    19681 aatagatgag aaaatagatt tagctcttga aatagctaaa agagagcgat tatttatgta
    19741 tcctatagat aaaaataaaa tttctcttta tgtaagtatt ccattttgtc caactagatg
    19801 tgtatattgt tcatttcctt caaattcatt aaaacaattt ggacatttaa aaagagaata
    19861 tgtttataaa ttgattgaag aaattaaggg gtttgccaaa gttattaagg atactaagaa
    19921 agaagttgaa actctatata ttggtggtgg aactccaact acacttgatg aggaagaatt
    19981 ggatttgctt ataaattcat tgttcaatga attagattta agtaaaatta aagaatttac
    20041 tgtagaagca ggtagacctg acactattac agaacaaaaa ctaagagtgt taaaaaaaca
    20101 caatgttagt agaattagta taaatcctca aactatgaat gatgatactt tagtaaagat
    20161 tggaagagag catagagtat ctgatttagt agattgcttt aatatggctc gtagaatggg
    20221 atttgataat ataaatatgg atataatttt aggtcttata gatgaagact taaatatggt
    20281 aaagaacaca ttggaggaga taaaaaaact ttctcctgaa agccttacag ttcatacatt
    20341 agccataaag agagcatcca acttaaatat aaatatggat aggtataaag aacatctaac
    20401 acaatatgaa gaaatggtaa aaatgataga tttatcaatg aaatatgcaa aagatatggg
    20461 attaaatcca tattatatgt acagacaaaa acatatgttg ggaaatttag aaaatatagg
    20521 ttatgctaaa gaaggatatg agtgcattta taatatacag attatggaag aaaaacaaag
    20581 taactatgca ttaggtgcag gtgctatatc gaaatttgtt tatgtagatg aagatagaat
    20641 tgaaagagta gaaaatgtaa aaaatgttga acaatacatt gaaagagtag atgaaatgat
    20701 aaaaaggaag aaagaagagg tatataaaaa tgttgactaa agctcctaga ggaactaaag
    20761 acataactcc aaaagaggct tacaaatggc gttatgtaga aaataaattt agagaaatat
    20821 gtgctttata tggatatgaa gaaatggtaa cacctatatt tgaacataca gaacttttca
    20881 agagaagtgt tggagatact acagatatag ttcaaaaaga aatgtattct tttaaagata
    20941 aaggtgatag agaaattacc ctaaagccag aaggtacagc aggtgtagta agagcattta
    21001 tagaaaataa attatatgca gatactcaac caacaaaatt attttatgtg acaccttgtt
    21061 ttagatatga aagaccacaa gctggtagac aaagacaatt tcaccaattt ggaatagaag
    21121 cacttggaag tgatactcct tcaatggatg ctgaaataat cgcactagct gttcaatttt
    21181 ttaatgaagt tggattaaat gatttggtag tgagtataaa ttctgttggt tgtccagttt
    21241 gtagaaaaga gtacaatgct ttattaaaag agtatcttga ctcaaaagct gatatattgt
    21301 gtgatacttg caatgagaga agagaaaaaa atccaatgag ggttatagac tgtaaaaatc
    21361 ctacatgtaa agaaaatatc aaagatatac catttattgc agaccatttg tgtgatgatt
    21421 gtaaatctca ctttgataaa cttcaagaat acttaaagga aatgaatata aactttgtaa
    21481 ttgataaaac aatagttaga ggcttagatt actatagaaa aactgctttt gaaattatat
    21541 ctaatgatat aggagctcaa agtactgtat gtggtggagg aagatatgat ggtcttgttg
    21601 aacaattagg aggaccaaaa ggtataagtg gaattgggtt tggactagga attgaaagac
    21661 ttttacttac acttgaaggt aatggaattg aaatagaaaa ccctcaatct acggatatat
    21721 ttatagtaac tataggtgaa aaagcaaata caagaagctt taaattgtta aaagacttga
    21781 gacaaaatca tataagtgca gataaagacc atatagagag aagtgttaaa gcacaattta
    21841 aatattcaga taaaataaat tcaaaattta caatagtaat tggagatgat gaacttaaaa
    21901 atgacactgc gacattaaaa aatatgaaaa catcagagca aactactgta aaattaagta
    21961 cattagttga agaattaaag caaaagctgt aaaataagaa agtatgatgt tttgtgtatt
    22021 agataaatat atagcattaa aattagataa aatggaggta gtatcttgga aactttaaag
    22081 ggtttaaaga gaactcacta ctgtggagag ttgagagaaa aaaacataaa tgaagaagtt
    22141 gtacttatgg gatgggtaca gaaaaaaaga aatttaggtg gtcttgtatt tgttgattta
    22201 agagatacaa gtggattatg tcaaatagtt tttgatacag atgtagataa agaagctttt
    22261 gaaaaagctg aaaaattagg ggctgaattt gtaatagctg taaaaggaaa agtatgcgaa
    22321 agacaatcta aaaaccctaa tatgccaact ggtgatatag aaatatttgc aactgaactt
    22381 agacttttaa acaagtcaga aacttcgcct atttatataa aagatgatga tgatgtttct
    22441 gaagcactaa gattaaaata tagatactta gatttaagaa aaccatctat gcaaagaaat
    22501 ttaaaattaa gacataaagt aatgaatatt acaagaaatt acttatcaaa caatagattt
    22561 tgcgaaatag aaacaccatt tttaatagct ccaacaccag aaggagcaag agattatcta
    22621 gtaccaagta gagttaatcc aggaaaattt tacgcattac cacaatcacc acaattatat
    22681 aaacaattat tgatggtaag tggtatggat agatatttcc aaatagttaa atgctttaga
    22741 gatgaagact taagagcaga tagacaacca gagtttacac aaatagactg cgaaatgtct
    22801 tttgttgagc aagaagatgt tatgagtatg attgaaggtt tgcttgaagc gatatttaaa
    22861 gaagttttag atgtagaatt ggcacttcca cttcctaaga tgacttatgc agaagctatg
    22921 tctaaatatg gttcagataa accagatact agatttggat atgagttaac agatatatct
    22981 gatgttgttt gtaactgtgg atttaaagtg tttgctgatg ctacacaacc aggaaaaagt
    23041 gttagaggta taaacgtaaa aggcaaagct gacgacttta ctagaaaaca aatatcatca
    23101 ctagaagaac atgctaagac atatagagca aaaggtcttg cttggatgaa agtaggacaa
    23161 gaaggagtaa cttctccaat agcaaaattc ttcaatgaag aagaaatgaa cgctatactt
    23221 acaagaatga atgctgaagt aggagattta cttttatttg tagctgataa aaactcaata
    23281 gtatttgatg ctttaggaca agttagactt gaagtagcaa ataggcttaa tctattggat
    23341 aaaaatgtat ataacttact atgggtaact gagttcccag tttttgaaga agatgaagaa
    23401 acaggcacat tctcagcaat gcatcaccca tttacatcac ctatggatga agatttagac
    23461 aagttagaag aaggtgataa gtcatcttta agagcgaaag cttatgatat tgtattgaat
    23521 ggttatgaaa taggtggagg aagtgttaga atatcaaatt ctgatgttca atcaagaatg
    23581 tttaaagcac ttggttttac agaggaaaga gctaatgaaa agtttggtta tttattagaa
    23641 gcatttaaat atggtactcc tcctcatgct ggtctagctt ttggtcttga tagacttgtt
    23701 atgttacttg caggaactga taatataaga gaagttattg cattccctaa aaatcaaaat
    23761 gctgtttgcc ctatgacaaa tgctcctaca ttggcggaag atgagcaatt agaagaatta
    23821 agtataaaag ttgatataaa agataatgaa taatatgtag ttgcaagaaa ttaaaatgta
    23881 aaaagtctgg atataaagta tatttatatt cagacttttt ttattagaat tattttatat
    23941 tttttctcta tatattttca taaattatct ttgtattctc agccaatata attaaaatac
    24001 tatgtttaaa aattttacat aaacttggtt gttttagaaa ctacttctgt aatgagtaca
    24061 cgattttaat taaagaatat ataacaaatt tgaaataagt ctaatatatg acttaataga
    24121 ttaaattaaa ataataaatt tggactattt atctatttat aacttaaatt atatcaaatt
    24181 gataacaaac tagatattat acttttttgt agaatagaat tacaattata taaagaaaaa
    24241 ttctattaat agtgaaaaaa aagtagaaaa aagtagaaat tattctttta taaagaaagg
    24301 gagggataat attgcaagag agagaagaat tagttttaaa tccagttcca aaagaggaaa
    24361 gagttggatg gttagctcca ttgtttaata tgcttggttc taacatagct atatctgaac
    24421 taatggttgg gggaacttta attttaggaa tgactctttc taatatgatt attacatcaa
    24481 ttatagggaa cttaatatta gttgcaatta ttatgataca aggttatata ggttacaaag
    24541 aagggttaaa tacatatgta ttagctaagg gggcatttgg agagatagga ggaaagtatc
    24601 ttatatctct attacttggt ataactagtt ttggatggtt tggtgtacaa gcaggggttg
    24661 caggactatc agtacaaaag atattcccaa gtgtaaacct aacattagtt acagttatac
    24721 ttggattatt tatggttgtt tttgcactat atggctttaa agctatggct aaatttaatt
    24781 acttagttat accaccactt atgatattaa tggtgtgggg agtttttaaa gctttttcta
    24841 cttatggagt tgaagctatt tataattata ctcctcaaac aactatgagt atggttgaag
    24901 gtcttaatat tgtagtaggt cttgttatag ttggtgcaat tatatcacct gatcagttaa
    24961 gatatacgag acgagtaaaa gatatttgga ttatcagttt tttaggtcta gggattattt
    25021 cattattcca acaagttgca gctggtgtaa tgtctatggg agcacctact tgggatataa
    25081 cagaagtatt ggctaattta ggttttggat gggtagcgtt tgttatttta atacttgcat
    25141 cttggtctac aaacgtatca aatgcttatt ctggtggatt agcattaaaa actatttttc
    25201 caaatgtaaa aagaaatgtt ttgactttag ttgcaggtct aataggtaca ataatagcag
    25261 ctactggtat aatatttaaa tttcagtcat ttttatcttt cttgggaatc gcagttcctg
    25321 caatagcagg aattatgtgg tgtgagtatt attttataca aggaagaact tataagcata
    25381 gagaaggaat aaattggatt gcagtaataa gttggttaat aggttttgca gcgtcgtatt
    25441 atagttcaaa aataaatttc ttaatacctc cgataaatgg aattgtagtg agtatggtta
    25501 tttattatat attaatgaat tgttttggaa tcaaagataa ataaaggggg caattacatg
    25561 ggtcgtaagg tgaaaatagg tataattcaa caacacagtg tacttggaaa tgttaaaaaa
    25621 aatatagaaa aagcagtaga gatgatagat gacttaggaa aacaaggggc agatataatt
    25681 tgtttaccag aactatttgc tacaggatat aatttagagt ctttaggtgg agttaaaaca
    25741 ttagaattaa taagagaaca taataagtac atagaggaat caatgtcaga agctgctaaa
    25801 agaaataatg tatacctgat atctccatat ggaaccttag aaaaagggtc tacacatgtt
    25861 tataattcag cagttatatt tgatagaaaa ggtaagatta tgggagaata ctgtaaaaat
    25921 catctgtggt cattagaagc agtatacttt aaaggagggg aaaaagtaga ggtatatgat
    25981 gctgattttg gtagatttgg tgtaatgata tgttatgatg caggttttcc agaagtatcc
    26041 agagaattga cattaaaagg ttctgaaata atatttatac catctgcatg gagaatacaa
    26101 gatgaagata tgtgggattt aaatgtatct caaagagctt tagagaacac tgtatataca
    26161 gtaggtgtaa atttagtaag taatgactct aatttaatac tatttggaaa gagtaaaata
    26221 tgcaatccta gaggtactgt aataactcaa ttagatacat acagggaaaa atatgtatta
    26281 tcagagattg atttagatga aataaaaaag tatagggaag atatccccta tttgaaagac
    26341 agaaaaagta gctataacat ataattctat actataatac aagttcaata taaaatgata
    26401 aaaagaggat gtatcaatat taagattaaa tttattatac tttgatatat cctctttaat
    26461 attatcaact ttgatttaaa tatttttaaa atagttgata agaaagtttt tgaacaacaa
    26521 cacagcttta gattcatagt tttcgctaaa ccatgaaaga tttaaatatc ttttaaattc
    26581 aatatctttg atatgtaaca acttaatctc agggtcttga gataatcccc atgattttcc
    26641 acatataaat ccaactcctt gaagtgattt tataagagca taaattgtat atggtgaatc
    26701 agactcaaat gctattttgg gtttgaaatt agctgattcg cacaggatat caattacctc
    26761 tctataattc tcacccttcg ttataactat aaagttttca tctgaaactt cgcttagata
    26821 tacttcatcc tttaatgata aaggatggtt tattgaaact cctaatagta tttcttcgca
    26881 tgttaaagtt atgctatttt ctgagtttag ctttgtaaat gaagaagata tgtacaaatc
    26941 aaaatcactt tttttataag atgatggcaa agtatgtgac acattaaaag taatattggg
    27001 atatagtttt ctgaaattac tcaaaagtga tggtaacaca ttagccgctg caggagataa
    27061 tatctttact tctcctgaat tttctaaaga agtgtcttgt aactcctttt ttgaactttc
    27121 tactaaatct aaaatgctat ctactctttt tagaaacaat cttccatact catttaaaac
    27181 aatatatctt ccttttctgt caaataactg aactccaagt tctttttcta aggaagatat
    27241 agttttgctt aaagctggct gtgctatatt taacttgtgt gatgcttttg ttatgtgctc
    27301 cattctagca acagtttgaa aatacttaag atgcaataaa tccatagtat aaaatacacc
    27361 tcttttaata tgacttgagt tatatcaatt ttataatcaa ctccacatta tacattataa
    27421 tataaagttt atataataaa ctacaatata ttaaaaagtt atttaaattg ttatttatat
    27481 tttgtacata atctaattaa aaaaagtaac tttaaatgat taactaagga aatattgtta
    27541 caaactttag ttatataaaa aattcatttg atgagcaaag tattaatgtc aagtaagaat
    27601 aaaagcttat tagataagaa aaataatatt ttataataat gatgttactt aaattattat
    27661 gttatactat tttaaatagg aaacatttag atactataat tttaggggga gtcaagatga
    27721 aattaactaa atcaatatca tcaatgctgt gcttatctct agttttaagc ttatcaccag
    27781 tagcatatgc aaatgaaata agtagaacaa cagttgagaa tactttagta ggtcaggata
    27841 ggtatcaaac tgctgttgaa gtaagtaaaa aaggatggag tagtgcaaat gaggctgtag
    27901 ttgtaaatag tgaatctatt gtagatgcat tagctgtaac accatttgct aaattaaaaa
    27961 atgctcctat attattgaca gaaaaaaata atctaaattc acaaactgag aaagaattaa
    28021 aaagattagg agttaaaaaa gtttatataa ttggtctatc tagttctgta tcaaatgatg
    28081 tgcaaaaaca gttagaatct aataatttaa ctactgatag agttggtggt agtgatagat
    28141 accaaacagc tttgagtata gctaaaaaaa tagaaggatt aaaagatata tcagaaatag
    28201 ctgtagtaaa tgggtacaca ggtcttgcag atgcagttag tatagcttct gtagctgcta
    28261 ctaatggaat ggttatactt ccagtatctg atgcatctgg tatatctagc tttaaagatt
    28321 ttatagaatc taagaatata aataaatcat acataatagg ctctactaat gctgtatcag
    28381 ataaaataaa acaaagttta cctaattcag aaagaatagg tggagcagat agaaatgaaa
    28441 ctaatggtaa agtaatagaa aagttttata catctaataa attaaacaat gtatttgtgg
    28501 ctaaggatgg aatgaaaaat caaaatcaac ttatagacgc tctagcagta ggtgtactag
    28561 catcaaaaga aaattctcct gtactaatcg taggagaaca attaagtgca acacaaaaga
    28621 gtgtattttc gaataaaaat gctacctcta taactcaagt tggtagtgga ggaaatgaaa
    28681 atgcatttaa agaattaaaa gatttacagg caaatagtaa caataagaga gtaatgtatg
    28741 taactaatac agataaggta aatataagaa atgatgctac aatagaagct tctgttatag
    28801 gatatctaaa taatggtgat gaagtagaag ttttagatgt gttaaaaact ggatgggtaa
    28861 aaataaaata caatgaaggt attggatatg ttagtgggtc ttatttgaca aataataagc
    28921 ctgataatag taatgaaaat ataaaaataa agtatgtaaa agaaaaagat ggtctaaatg
    28981 ttaggaaagg accaagtaca gaagatgaaa agataggtca cctttcatat ggaagcaaag
    29041 tagaaacaat agaaatgttt gctactggat gggtaaaaat aaagtataat ggtggatatg
    29101 gttatgtaag taatgattac ttatctgata caccagttat ttaattataa gtaacaataa
    29161 aaaaactgct ttaatttgta aagcagtttt tttataaaaa tttaagtcta ataactattt
    29221 ctattttgat accttaattc tgggatttat tattatggat attatcaaca tacaaacact
    29281 ccaaatccag tgtctaattc caaatttaga tattaataaa ttcagtcctg ctatggtaaa
    29341 taagcagtat ttaattacaa ttaaaagtgt atttatagaa agccccctca tagaaagtat
    29401 cctaaatcca ataatggaca ttctaagtcc tataaatgcc aataatatac ctgcaatatc
    29461 ctgaattaag taatagtatt caaatttcat attcaatcaa ctcttctctg agcagtatca
    29521 aatatttcta actcttcaat ttcatcatct gtgtatgtaa gcatagtaaa accagttaca
    29581 ccataaataa atgaaattat aggtgtaatc caacttaaga aacaatatgg aataaattct
    29641 agtggactta caccaagagt ctgagcacag aaaacagcat ttgaattcca tggtattaat
    29701 ggaccaccaa gtgtagctgt atcttctata attctagata gattttgtgg ttttaattta
    29761 aattctttgt atattggaga cattagagtt ccgacaaata cagaagtgaa catcattgat
    29821 gaactaaatg catttgcaaa gtagcttact ataaaagtag acccaaccaa tttagttctt
    29881 gattttccta ttttagttat aagaggttga agcagtactt ctaagaatcc agttttttgg
    29941 agaataccag caataacgaa aactacaaat actagtaaaa cggtttcagc catactcatt
    30001 attccaccac gatttaatag tttgtcggca tagacaaatc cagtatctat agtaaatcct
    30061 gatagcatgt aatttaaaac atcacctatt ttttctcctt cttgaaatac tgctataata
    30121 agtcccatga tagctgaact tagtatagat ataatgggtg gcttttgtag caataaaagt
    30181 aataataaaa atattattgg aatcattgct actaaaccaa tatgaaagtt tgaatttaat
    30241 acatccttga tttgatttat ttgaacatag tctatagtat tatttgaata cttaaatcca
    30301 attactgtat acagaactat acatattacg tatgcaggtc ctgtggtata aaacatatgc
    30361 ttcatatgtg taattacatc tgtcttacaa actgctgcag ctaagtttgt agaatctgat
    30421 agaggtgaca tcttatctcc aaagaaagca ccagaaatga ctgcaccagc tgttaaacca
    30481 agaggaatac ccatactagt accaatactc atcattgcta taccagaagt tccaagagtc
    30541 ccccaagatg taccagtagc aacagaagtt aatgaacata cgattaatgt tgtaagcaga
    30601 aaatatttag gggttattat tgttagtcca ctatatacta ctgttggtat tgtaccagca
    30661 gctatccaag ctgctattag tacaccaaca gaaagtataa ttaagtttga ttgaaaggca
    30721 tctttaccaa cttcaaatcc aaaagcttct atttcatcat tagtataacc aagtttcatg
    30781 gctgcaggaa ctactataag ccatgaaagt aaaaacattg tagttataga agcattaaat
    30841 acaactatac caactgatgt tagggctatt attgcaagca ttactaacaa ggaatatcta
    30901 aatgaaggta tttttctttc tgtattcatt ttcatagtgc aaattctcct taaatatttt
    30961 tagttaattg cattttttat tcttataaga gcttcttcaa gcatacttct tggacaagct
    31021 aagtttattc tttgataccc acttcctcca atgccaaagc tgtttccttg atttagagca
    31081 actttacctt tttgtacaag tatactttct agttcctcat cacttaatcc aagtgcacta
    31141 aagtctaccc ataataaata agtaccttct ggttttctaa ctttaagttt tggcatattt
    31201 tcatttatat attttatagc aaaatctata ttagattcta aatattctaa aaatgattct
    31261 agccaagatt caccattatt atatgaagct tcagttgcaa ctaagctaaa gcaattattt
    31321 ctctttatat caattctggt aaaggcatca tctaacaatt tatagtcttt ttcatcaggt
    31381 agtactacat atgatgattg aagaccagct atattaaaag ttttagttgg agccatacaa
    31441 gtaattgtat ttttttcaaa ttctttagaa atagatgcca taggaatatg cttatgtttt
    31501 tttaatataa tgtcactatg tatttcatca gatatgattt ttacattatg cttaagacaa
    31561 atatctccta gtttttttaa ttcatctttt gtccatactc taccaactgg attatgagga
    31621 ttacaaagta tgaataattt tacatcttta atcttgtttt caatgtcttc ataatccatt
    31681 atgtagtttc cattttctag tttttgtaat gggcttataa ttagttctct attgttattt
    31741 tttacaacac tattaaaagg gctgtaaact ggttcttgta tcattatttt atcatttgct
    31801 tttgtgagtt catttattaa aagacttata gctgggataa cacctggact ataaattaac
    31861 cactcacttt ttattttcca gttatgtctc ctatctagcc agtttactat agattcattg
    31921 taagaatctg gtcttgttgt gtagccataa atctcttgtt ctagtctatt ttttaaagaa
    31981 tcaattatac aaggtgcagc cttaaaatcc atgtcagcca cccacatagg aagtaaatca
    32041 ttagttccat actttttttc catttctgac catttagatg aaaaattatt gcttctatca
    32101 acaatctcat taaaattata attcattgta aatacctcca gtaattttaa ttttatgtta
    32161 ataatattaa gcaaacaacg tgccaatatt gataataaaa aatatttatt cacaagttaa
    32221 tatttaatat taagtaattt cagagcatag ataagataaa aatattaatt atacttaaaa
    32281 ctttgtggaa aaggttattt ctaataaatt tttaaatata tgaaaatgta attaaatagg
    32341 ttaaataggt ttaactgttt aacttgttag acctatttat attttttaga aattctactc
    32401 cttttgatgt tataattgaa cctccacgac caacaagtat gtctacaagc ccaaagtctt
    32461 gtagttctaa aagcatattt ctaatctgtt gttctgtcaa aaatctattt tcctctaaag
    32521 ctattttaca taagcttttt ctacctattc tttcttttaa atcataagcc ttttttaaat
    32581 tatccaatat aaatatgtat tcatctagtg actttttaaa attatagtcg tatttcatga
    32641 tgtttttgtc tttgccaatg ttgaattgat acttttttat attatcactt actttattac
    32701 aaacggttct actataattt gaatctatag tatccaatat atattcaggt aaatcacata
    32761 tttctataat atctttttcc aagtaacaga agtattctcc taagtttctt aattctctta
    32821 catttccttc ccatctatac attttaaaga tttcttttac ttcctcactt agaataaaat
    32881 tacatggaat atcatatttt aatgaaccaa aaatttcaaa tatatcagca ggtctttccc
    32941 taagtggatg tattttaagg ggaagaacat tcaacctaaa atataaatct cttctaaaca
    33001 tatttttaga tacaagttct tttaaatttc tattagtagc tgcaattatt cttacatcaa
    33061 tatcaatgac attattagaa ccaatacgtc gtacttgttt ttcttgtatg actctaagta
    33121 atttagattg agaatttaaa tccatttctc caatttcgtc taaaaatata gttccattgt
    33181 tggctaattc aaataaacca attttcccac cttttttagc accagtaaaa gccccttcat
    33241 catagccaaa taattcactt tgcagtagat tttcttgaaa agtactacaa tttactgcga
    33301 caaaaggacc atcttttctc ctagaagcac tatgaatgga ttgagcaaat aattctttac
    33361 cagtaccact ttcaccaatt ataaggatag atgaatttga ttgagccatt ttattagcaa
    33421 ttttttttgt attaatagtt tgtatactag aacctaatat atcatcaaat gtgtatttac
    33481 tgatattacc agagtttact agttgtgctc ttaatttagc ttgctttttt tctaattgag
    33541 aaaactttgt catctttagt ataaatccat caaatacatt taattgtata tgtttaatct
    33601 ctaaatttat atcaatatga tttattttaa tcaatttctc aaaaaatgga gttttattgg
    33661 tcaatatgct ttgaaaattg atgtctttta tgcaatcacc aacaaatgag tcaatcattt
    33721 catttgcatt tatagacaaa atttttctag ccatatgatt atagaattga attattccat
    33781 cgttatttgt gcatataatt ccatcatcaa ttatagatag caacaaatca aattgtcttt
    33841 caaacttatt tgcatctaac aataactcac ctgtactata acttgtagga attatttttt
    33901 ctacgtattt ttttattagg tcatctttta ttagatgttt taatttggtt tttgtagcaa
    33961 tttcaacaat acaacttaaa tctattattc tgtattttaa atctataact tcacaatttt
    34021 taaacttagg caagattttt tcgccaggag ttaggattac agcatctgtg ggagtaaact
    34081 caatctctgg atagcagggc aggatattta tattgtctat tcctaatcta tgtattaaag
    34141 aaattgtttc tattgccata tctttactta agttatatac ataaataatt ttatcttttt
    34201 ctaatttgga tatcatatca atactgttct tttcaaatgt caacttggga acaatgattt
    34261 gagcatcata gtgagataat tttactattt catcatattt taaataagca gataggagta
    34321 taagtttttc tttaaaaaac tttaaatttc cctcttcaaa tgagtataaa tttatcttta
    34381 gattatcacc taagaattgt cttatttgat tttcataaat ttctccagcg tcttttttta
    34441 aacttacaat agctaattcc atttgtttca ttttaacctc tcatacaaat ttgacttatt
    34501 ttagattaca taataattaa attagattaa ataatattat ttaattatta ttataaattt
    34561 attatagact acacattgaa gtaaaagaag tagctaaaat tattcctttt atttttgttt
    34621 tttgtatcta tttggtataa tagtattaga gcgtataata atttaggcaa aattttttaa
    34681 ggaagactag aggagaatat aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt
    34741 caccaaataa taaattagct ataaaatatg atgacaatat gaatatatat ccatgtcgtg
    34801 cagaatgtaa ggaaaaaatt gaagaatata tagcaaaaaa acctacatat aagtttataa
    34861 atatattaga ggttctttta ggtgtgggcg gaatattctg ctttttgagt aaatggacta
    34921 ttgcagccca agttgtactt ggaattgatg cactggttat atttttattc ccattggcag
    34981 gatttaagat gaatggaaag ttaagtatgg aacaaactaa gaatcaaagt aggtctattg
    35041 caatatcaat tttggcattt atagtaataa taactgtatt atattttaaa caagttggta
    35101 agtagagcat aattgtataa gacatcatta gagtatgagg tgagtttatg gaagaaagaa
    35161 aattagcacc aaacaatgag agggaagctc tcataaaaag attaaaaaga atagaaggtc
    35221 aagttaaggg tatacaaaag atggtggaac aagaacgtta ttgtgttgat atattggttc
    35281 aaatatctgc tataaggtct gctataaata gagtaggcac aattatctta gagaatcata
    35341 taaaaggatg tgtagcagaa agcattaaac atgatgatgc tgataaaact gaagagatga
    35401 taaaagagct tatggatact ataaataaat ttacaaaata aggaggcggt tagttatgaa
    35461 tcaacaagga tttattatgg aaattgttga tgatagaact gcaaagttaa aaatgcaaag
    35521 acattcagct tgtgctgctt gtggaaaatg tgctactaca aattcagcag agtcaaaaga
    35581 aatattagta gaagtagaca acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag
    35641 tatggataat atgaatgtat taaaagcagc tgtaatggct tatataatac ctcttatagc
    35701 acttcttgta ggaacaatag ggacttatta tatattgggg gcaattggaa tgacaactgg
    35761 agttgaagcc ataagtggat tagttggaat ttgtcttaca ataatctgtt atttatactt
    35821 aaagaagaat gacaaaaaat ttagagacag tagagaattt ataccagtaa taacaagaat
    35881 attaattgac ctttaagata attataatta aaaaatagca atgtactttt taaataaata
    35941 ctcaatacgt atttgtagag tttaagtaca ttgctatttt tatattttta ttttgatttt
    36001 ctgtaaggaa taaagttttt tctgattttt aaaagattca atgttcttac aaaagaagtt
    36061 acaactaata caccaagtgc tattgcagca cttagaaata gagcatctgt accttttgac
    36121 atagccaaag aataattttt ctgaatcaaa gacaacattg tattatatag cccaattcct
    36181 ggtattagag gaagaatacc tggaattacg aacacaatag ctggttgctt tagttttctt
    36241 gctaaagtct cactgcacgc tgagaccaaa gcagcagcga taaaacctgc aaaaactgca
    36301 tttgtagtaa aattaaataa ataaacatat acaatccaac caattccgcc agttactcca
    36361 gctggaagaa gtagatatag tggagcatta aagaaaacag caaatccagc agttgcaaca
    36421 cctgaaaata aaaaatgcaa aatcatagac atatcagtca ttagaaagga cctcctattc
    36481 tataccatgt atcaaggaca aaaccaactc cagaagcaat tgcaataata atcatagcag
    36541 catcaaaagc acgagaaatt cctgatatta aattccctga tattaaatct ctaatagctt
    36601 ttataaaagc aactcctggg agtagtggca ttatagcgcc gactataagc attttagggt
    36661 catctaaaat gcctatttca actagtagaa ctcctgaaag tgcaattaaa aaagaagata
    36721 ctaaacagga aaatgcagat attgcactta tcttcatcgt tttatcataa gtaattgcag
    36781 caaagattgc tattataaac gtaagtataa aatttaatac ataattccct ccaagcaaac
    36841 aagcaaaaca agcagatgca attcctgtac aagagtaaaa taaccataaa ggataaggtt
    36901 ttacatcttg gatttcataa agacgtgcaa tggcattatc aatatcatac tctttgtcac
    36961 taacaaattc tcgagaaaaa ctgtttaata aagaaatctt atttaagttt atacctcttg
    37021 attgaatcgt tttcataaac gaaaagccat caaatttctc atctgaaata attattactg
    37081 taggagttgt aaaaatattt acatgataaa accctcttga cctgcatatt ctaaggacac
    37141 tgtcttcaac cctagatgtt tcagctccat tttttatcat cagttcccct acaaatagag
    37201 ctagttttag aatatttttc ttatattcaa gttcctcatt ttgattcata aatacacctc
    37261 caaattaaaa aacgtaatat tgaaattata tcaaattttt aagtaatatt ccttaatatc
    37321 tatataaata tattatcttt tttttgtttc aaaatagtca catttaaatt at
//
LOCUS       DAMJYC010000033        35702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJYC010000033 DAMJYC010000000
VERSION     DAMJYC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.034"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(57..1232)
                     /locus_tag="PC472_001796"
     CDS             complement(57..1232)
                     /locus_tag="PC472_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2727122.1"
                     /translation="MREVVIASAARTAVGSFGGVFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(1319..2164)
                     /locus_tag="PC472_001797"
     CDS             complement(1319..2164)
                     /locus_tag="PC472_001797"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2727123.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(2262..3059)
                     /locus_tag="PC472_001798"
     CDS             complement(2262..3059)
                     /locus_tag="PC472_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2727124.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(3078..4088)
                     /locus_tag="PC472_001799"
     CDS             complement(3078..4088)
                     /locus_tag="PC472_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2727125.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(4107..4889)
                     /locus_tag="PC472_001800"
     CDS             complement(4107..4889)
                     /locus_tag="PC472_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2727126.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(4948..6084)
                     /locus_tag="PC472_001801"
     CDS             complement(4948..6084)
                     /locus_tag="PC472_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2727127.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(6793..7260)
                     /locus_tag="PC472_001802"
     CDS             complement(6793..7260)
                     /locus_tag="PC472_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2727128.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(7339..7881)
                     /locus_tag="PC472_001803"
     CDS             complement(7339..7881)
                     /locus_tag="PC472_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727129.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(7933..8619)
                     /locus_tag="PC472_001804"
     CDS             complement(7933..8619)
                     /locus_tag="PC472_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2727130.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(8619..9479)
                     /locus_tag="PC472_001805"
     CDS             complement(8619..9479)
                     /locus_tag="PC472_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727131.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            9614..10414
                     /locus_tag="PC472_001806"
     CDS             9614..10414
                     /locus_tag="PC472_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2727132.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            10439..11008
                     /gene="yfcE"
                     /locus_tag="PC472_001807"
     CDS             10439..11008
                     /gene="yfcE"
                     /locus_tag="PC472_001807"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2727133.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(11344..12396)
                     /locus_tag="PC472_001808"
     CDS             complement(11344..12396)
                     /locus_tag="PC472_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2727134.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(12603..13829)
                     /gene="pepT"
                     /locus_tag="PC472_001809"
     CDS             complement(12603..13829)
                     /gene="pepT"
                     /locus_tag="PC472_001809"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2727135.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(13920..14972)
                     /gene="ytvI"
                     /locus_tag="PC472_001810"
     CDS             complement(13920..14972)
                     /gene="ytvI"
                     /locus_tag="PC472_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2727136.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(15121..16374)
                     /locus_tag="PC472_001811"
     CDS             complement(15121..16374)
                     /locus_tag="PC472_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2727137.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(16505..20032)
                     /locus_tag="PC472_001812"
     CDS             complement(16505..20032)
                     /locus_tag="PC472_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2727138.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYKKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(20019..21230)
                     /locus_tag="PC472_001813"
     CDS             complement(20019..21230)
                     /locus_tag="PC472_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2727139.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(21276..25103)
                     /gene="addA"
                     /locus_tag="PC472_001814"
     CDS             complement(21276..25103)
                     /gene="addA"
                     /locus_tag="PC472_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2727140.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(25103..28570)
                     /gene="addB"
                     /locus_tag="PC472_001815"
     CDS             complement(25103..28570)
                     /gene="addB"
                     /locus_tag="PC472_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2727141.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(28560..29390)
                     /locus_tag="PC472_001816"
     CDS             complement(28560..29390)
                     /locus_tag="PC472_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2727142.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(29499..31718)
                     /locus_tag="PC472_001817"
     CDS             complement(29499..31718)
                     /locus_tag="PC472_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2727143.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(31711..33624)
                     /locus_tag="PC472_001818"
     CDS             complement(31711..33624)
                     /locus_tag="PC472_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727144.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRVEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEIEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQDSILYSI
                     LLKISHKGDKNYD"
     gene            complement(33852..35666)
                     /locus_tag="PC472_001819"
     CDS             complement(33852..35666)
                     /locus_tag="PC472_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2727145.1"
                     /translation="MTLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPEYLNVWELQ
                     GITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSSPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRYETSLLIAK
                     EIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTDNELEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKIGGGLTS
                     KVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKDYNLNTSLA
                     TTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNKQGHGVEVF
                     YKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRSNDMPAV
                     LVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
BASE COUNT        10915 a         6411 c         3612 g        14764 t
ORIGIN      
        1 tcttacaggt taaattcata attaaatatt taatttgtaa atattataat ctaaaactat
       61 ctcttaacta ttaaagtagt tcccattcct ccacctatac aaagtgtagc aagaccagtt
      121 ttagcatctc ttctcttcat ttcatataaa agtgtagtaa gtattcttgc tcctgagcat
      181 cctattggat gtcctatagc tattgctcca ccattaacat taactttatt catatctata
      241 tttaagtctc ttattacagc tacagattgg gcagcaaatg cctcattagc ttcaactaaa
      301 tctatatctt caatagtcat attagcagct tctaaagctt ttttagttgc tggaactggt
      361 ccatatccca ttattttagg gtcaacacca gctgttccat aagaaactat agttgcaaga
      421 ggctctattc ctagttcttc agctttttct ttagccatta ctactagcat agcagcacca
      481 tcatttattc ctgatgcatt accagcagta actgttccat cttttttaaa tgcaggtctt
      541 aacttagcaa gtttctccat tgtagtgcca ggcttaatat attcatcttt atctactaca
      601 gtgtcacctt ttcttccttt tataacaaca ggaactattt cttcatcaaa ttttccttca
      661 gcttgagctt tttcagcttt attttgactt gcaagagcta attcatcttg ttcttctcta
      721 gttatattcc attgctctgc tatgttttca gcagtaatac ccatgtgata gttattaaat
      781 atgtctgata atccatcttt tatcattgaa tcaacaaaag cagcatcacc cattcttgca
      841 ccatatctcg cacttggtac taaataagga gacatactca tgttttcagc tccaccaact
      901 aacattatat cagcatcacc taatgctata agttgagatg ccattgaaac agatcttaat
      961 ccagaaccac aaactatatt tatagtcata gctggttttt ctactggtat tcctgctcct
     1021 aatgctattt gtcttgctat attttgtcca agacctgctg taagtactcc ccctaaaaga
     1081 gattcatcta tcatatctgg agttatgtta gctcttttta tagcttcttt agctgctgtt
     1141 acccctaact ctaccgctga aactgattta aatactcctc caaaacttcc tactgctgtt
     1201 ctagctgcac tggcaattac tacttctctc atattcatta cccccattaa attagatttt
     1261 tcatatatcc gaaattgttt tttaaacaat tttatttaaa cttttaattc ccaaattctt
     1321 atttattata atcatagaat cctatcttag tttttcttcc taattgatta gctctaacca
     1381 ttttagctaa aagtggatga ggtctatatt tagtatctcc aaactcagta tataaaacgt
     1441 tcattatagc taaaacaaca tctaatccaa ttaaatcacc taatgctagt ggtcccattg
     1501 gatggtttgc tcctaatttc atagcttcat ctatttcttc tttacttgca acaccatctg
     1561 catatatacc aacagcttca tttatcatag gtataagtat tctatttact acaaatccag
     1621 gagattcaga tacatctact ggtactttat tgatactctt agataattca aatactgtat
     1681 caaaagtaac ttttgatgtt aactgaccac ttataacttc aactaatttc atcataggaa
     1741 ctggattaaa gaaatgcatt cctataactt tatctggtct cttagtagaa gaagctattt
     1801 ctgttataga taatgatgaa gtatttgttg ccaagatagt atcttcttta cataattcat
     1861 ctagtaactt gaaaacatct ttctttatat tcatgtcttc tacagatgct tctattatta
     1921 aatccatatc ttttaaatct tcatagttag tagttgaact aacatgactt aatatttctg
     1981 cttttgtagc ttcatccatt tttcccttag taactaactt agttaaattt ttatctaata
     2041 aagctaaaca tttatctata gcaccttgag ttctactctt taaacataca tcatgtccac
     2101 aacttgcaaa agtttgtaca ataccacttc ccatagttcc actacctatt acagctaatt
     2161 tcatagttat aacctcctgt tattttagta tttatattct gttaactaga ttaaaatact
     2221 atttaatcta gttaacagca aatatatttt taattagatt tttacccttt tataaagtta
     2281 gcttctctct tttcaacgaa agctgacatt ccttcttttt ggtctttagt tgaaaaacaa
     2341 agaccaaata aattagattc tatatctatt ccagtattta tatcagtttg agcaccaagt
     2401 tgtattgctt ctttagagta tctaactgca agctgagcat ttttagctat tatcttagct
     2461 aatttctcaa cttcttctat taaaatgtct ggctcaacga ctctatttac tagccctatt
     2521 ttttcagctt catcagcttt tataacttga cctgtaaaga ttaattcttt tgcttttgcc
     2581 attccaacca atcttgtaag cctttgagtt cctccatatc ctggagttat tccaagagtt
     2641 acttctggct gaccaaattt agctttagca gatgcaattc ttatatcaca tgccattgca
     2701 agttcacatc ctccacctaa agcaaatccg tttacagcag ctatcactac ttttttacta
     2761 ttttctattt ctccaaaagc ttttgctcct aagatactaa aatctttagc agctacagca
     2821 tctaaatctt tcatgtatgc aatatctgct ccagctacaa atgcctttcc ttcccctgtc
     2881 aatattacaa catcaatagt ttcatcatta ttaatatcta caaatacttc ataaagttct
     2941 tctaaagtct ttgaatttat tgcattaagg gctttaggtc tattcatttt cactgtacat
     3001 atatttccat ctacttcaac agctacattc tcataaactt taacatcact tgtactcata
     3061 ataccctccc caagaaatta gttagctaaa acttcacctt tttcttttgc aacacttaac
     3121 tgactgataa gttctggaag cactttatga acatctccaa ctataccaac atcagcatat
     3181 ttaaatattg gagcttctgg atttttattt atagcaacta taaactcagc atcttccata
     3241 ccagctatat gttgtattgc tccagatata ccacatgcta tataaaggtc tggtcttaca
     3301 gttttaccag tttgaccaac ttgtcttgct ttatctaacc aacctgcatc tatagtggca
     3361 cgagaaccag aaacttctcc acctataatt tcagctaatt cataaagtat gtctaagttt
     3421 tcttttccac ccattccacg tccagcagaa actaatatct tagcatcttc tattttaact
     3481 tgttttttag cttcttttat tacttgtaca acttgaacta atttatcagc atcattaaat
     3541 tctaccttga aacggttaat tacagcttct ttagtttcat caggttcatt tttcttcata
     3601 acccctggtc taactgtaga catttgaggt ctgaaatctt tacaaactat tgttgccatt
     3661 atatttccac caaaggcagg tcttgtcatt aataataatt ttgtatcttc agctactgca
     3721 agacctgtac agtcagcagt aagacctgta tgtattctag cagaaactct aggagctaaa
     3781 tctctaccta ttgaagttgc accaaataat acaactatag ggtcagctgc ttttattgct
     3841 tcataagctg cttttgtata tggttcagtt gtatacactg ctaaagcttc atcatctact
     3901 actattacct catctgcacc atagtgtgct aatgtatcta ttaaaccttc taccttacta
     3961 cctaaaagta atgcagaaac ttttgtatca taatcttttg ctatttctgt agcctttcct
     4021 agtaattcta aagaaacagt ttgaattaca ttttctcttt gttctattac tactaaaacg
     4081 ttacccattc tttatccccc cttaaattat atgatatact tctcttttaa tttatctatg
     4141 ataattccag ctgatgtttt cgcatcttca ttgtatattg taccagctgg tttaactgat
     4201 tttgtaaatg atttaaatac actagttgga gaacctttaa gacctaaatt agaagggtca
     4261 acttctatat cttttacagt ccatgtttct actacatcat tttcgaaagc atcatatatt
     4321 cttccaactt tcatgtatct tggtgtgttc atatctttaa gagttgttat aaggcatggc
     4381 attttaactt ttaagtcatg gcaacaatct tcaaattgtc tttttactaa tacatattca
     4441 ccttcagttt ttatttcttc agcatatgtt attgatggaa gatttaaatg ttcagctatt
     4501 tgaggtccaa cttgtgcagt atctccatct atcgcctgtc ttccagctat tataatatca
     4561 aaatctatat tttttaatgc tcctgctaat gctgatgaag ttgcccaagt atcagcacct
     4621 gcaaatgctc tatctgttaa taatatacct ctatctgcac ccattgctaa agcttctttt
     4681 aaagccatat ctgcttgagg aggtcccatt gttataacag ttacatgagc acccatttct
     4741 tcttttaatt ttatagcttc ttctaaacct gctttatcat cagggtttat tatacttggt
     4801 actccatctc taattaaagt acctgtatta ggatctagtt taacttctgt tgtatctgga
     4861 acttgtttta tacaaacgac tatattcata aatccttcct ccatatctat tttaatatcg
     4921 acctattgtg tatattttaa tatttatcta ttttaataat tttcctgaaa taaccattct
     4981 ttgaacttca ctagttcctt catatatttc agttatctta gcatctctca tcattctttc
     5041 tactggatag tcacgagtgt atccatatcc tccatgaagt tgtacagctt ttgtagtaac
     5101 ttccatagct gtttcagctg caaataattt tgccattgct gcttctactc cataaggttt
     5161 tcctaagtct ttatttatag ctgcttgata tacaaggtgt ctagccgctt gtaccttaac
     5221 ttccatatca gctaattgga attgtgtatt ttggaatttt gataatggtc taccaaattg
     5281 tactctttct tttacatatt taacagtttc atcaagagca ccttgtgcta aacctaaagc
     5341 ttgtgcagct ataccaattc taccaccatc aagagtagac attgctatct taaatccttg
     5401 accttctttt ccaagtaaat tttctttagg tattctgcaa tcctcaaata ttaattcact
     5461 cgtagctgaa cctcttatac ccattttctt ttctttaact ccaaagctaa acccaggagt
     5521 tcctttttca actataaatg ctgatattcc tttgttcccc ttagatttat cagtcattgc
     5581 cattactaca tatatgtcac cagctattgc gtttgttata aatatttttg agccattaag
     5641 tatgtattca tccccgtcta aaacagcagt tgtttgttgg ccagacgcat ctgtaccagc
     5701 attaggctca gtaagaccaa atgctcctaa tttttctcca cttgctagtg gtcttaagaa
     5761 tttttgtttt tgttcttcat taccatattg atatataggc catgagccaa gagatgtatg
     5821 agctgataat ataactcctg tagtaccaca aactctagac aattcttcaa ctgccattat
     5881 atatcctaca gtgtctccac cttctccacc atattctttt ggatatggta tacccatcat
     5941 tcctgctttt gccatttttt ccactgtttc ataaggaaat ctttcttctt catcaagttc
     6001 tgttgctaaa ggtttaactt cattttcagc gaagcttaca tatagctctt taagcatctg
     6061 atatttttta gaatttaaat ccataatagc ctcctcagta ttttataaat attttatttt
     6121 catttattaa ttaaaatctc ggcaacatct ataataaggt atttgaatat acgtcgagaa
     6181 atttataacc ttaatcaaac tacgctctat atttatatgt gattatacta accaattatg
     6241 acattaattt aatcataaat tgttaattta aaatcaacta tacttaaaaa aaatctcgct
     6301 ttttggaatt ttgtgaaatt tcaagttaga ataagatttc tttcacactg gttagagata
     6361 aaataatttg tttacgatat ttatattagg gtatattaaa cattaaatat tgtctgtaca
     6421 tctgtttttt agccatgtac agaagatttt tatcgtataa aaattatttt aatagagcta
     6481 aataatcata tttttatctg actatttttt atttttaaat atcttacatc tataatagta
     6541 tcactcaaag gataattttt caataatttc taattatttt tttaacaata tttattacat
     6601 tttttccact tttttgtcat ttttttattg tttcgaaaaa aaatcagaat tttttaactt
     6661 tattttggat tattagttta aattagattt tcctcttaga tttataaata tttacacaaa
     6721 aaaactacct ctgtatagat tgtatacaga ggtagttttt tactaattcc tactatagtt
     6781 tactatattt ttttaccaat tcttacaaac atctacccat ttttcacact tagaaaactt
     6841 ctccatttct tcacatgtaa gttctattgc agagttacta ctgccacatg caggaaatac
     6901 tgtatcaaat cttttcattg aatcatctaa atagactttt atagaatttt ttaaaccaaa
     6961 tggacagact ccaccaatag catgtccagt aaactccaag acttcttctg gtgtcaacat
     7021 tttagcctta caattaaatt cagccttata cttcttatta tcaatcttag catcaccagc
     7081 tgtcacaatt aaaattgcat catctcctat tttaaatgag agtgtcttag ctattcttgc
     7141 tggaataacc ccagctgctt cagctgcaag ttccacagta gcactagatt gttcaaattc
     7201 taatatactg tcttccttgc caaattgttt aaaatactct cttacattac ttattgacat
     7261 taaatacccc tcctagtctg aacttttata aatttcatat tattcttaat tatatctata
     7321 attgatttat ttttcaaatt attccataat attagcattc catgtatcat tattttttcc
     7381 aactactaga taaagtcctt ttgccattgg aatattgaca atatattctc ctttatactc
     7441 tacactcata ccagattcat taaagtacct ataatcaaaa tttagtcctt ttggaattac
     7501 aaaatcaaca taaccttgat tatttggttt tattccatat ttcagagttc tttcagttat
     7561 agaatttttt tcattaaata aatctacatt tacatatgca gaacacttat tcattccctt
     7621 tttattttcc acacaatttt caaacttcat tttagcactg tttaatttat aactatattt
     7681 actattattg cttattttaa atcttacaat agtaccaaca tctctacttc tcctgctatc
     7741 aaaatcaaaa acaatttcct ctaaatgatt tatttcataa tctttgacac ttaccatatc
     7801 tctatatact aatttattta tagctatgaa tagtgtaaaa atccccaaaa taaaaaatga
     7861 aacttttata aacttcttca tttttagtct ccttatattt tgtgctattg tgctataata
     7921 ttaatcaaaa ttttaataat taaacttatt cttttttgaa atcttaatag aaaaatacat
     7981 tgatattata aaaattatta ttgttaaaaa tgtcatacta tataaattta tatacttagt
     8041 attagctata tcagttacat aatttgaaag atatactcct ccaactttac tataattaaa
     8101 atgctttact tgatatacaa tcatactaat catcttcaaa agacttccat atacaaagta
     8161 aattaaaata cttactataa aaccacaaac tttcattttt ccatatccaa acttaaaaat
     8221 taaaggcagt aaaatcgtca aaattgctaa aaatatattt acagaaaaaa atacatcatt
     8281 taaaaccata tttctaaaga acactagtga tgtcaattta cttagtaagg acacaattat
     8341 tgttacagaa attattaatc ctacacctaa taaatatttt gagtaaacta tatcttctat
     8401 actaactggc atactcctta taaaaatcct tgaatcattt tcataatcac actcaaatgt
     8461 atttataaat atcaaataac taacaacaat acttactgta taatatgaaa ttgaattaag
     8521 gaaaaaataa aacacaaaaa atagcaatat gtactttaaa ttacggatac catctgaact
     8581 aatccctatt attaaatctt ttcttattaa attgaacatc atctcacctc atttacataa
     8641 taatacatta gttcttctat agtaggattt ttgaccttat gcttatcaat ttctatcaat
     8701 aggcttttta ctttcaagct atctccaact ttaacaagtg cctcattata atatttatac
     8761 tctttcacac caactatgct ttctttaatt gcttctaact ctgctttatt tgccctaatt
     8821 atcttatatt cttgttctat taattctttc ttaacagtaa aaatctgatt tcctctattt
     8881 ataaaaacaa gataatcagc aattttaatt aaattatcag tattatgagt agaaattatt
     8941 acactactac ttccattttt cacaaaatct tgtaagtact gcattatttc agttcttaca
     9001 attgggtcta taccagcagt tggttcatcc aaaatcaata acttagcatt atgactaagt
     9061 gcgcttgata acataagctt ctcagattga cctttagata aattttcaat atgcatattt
     9121 tcttctatat caaacttatt aaggtattca tcaaaccttt ctctatcaaa attcttataa
     9181 aataaagaaa ctatttttct aaattcctta acctttaaat gcggataaaa ttgtaaagaa
     9241 tcatatacaa aaccaatatt gtttttaaag cttattggag aattttcaat agtttcatta
     9301 caaagttcaa tttcaccttc atctgcatca ataatcccca ttataatttt tattaaagtc
     9361 gtttttcccg aaccatttgg tcctataatc ccaacaatat ccccttcctt taactctaag
     9421 tttatattat ttaacttgaa ttttcctaat ttttttgtga tattccttat gctaaacaca
     9481 tcttcacctc ttatgcattt ttttataccc acctatacat attttaacac attcactaca
     9541 aaggtcattt ataaatttat aaattaagaa ttataaaact atgttataat acaaagtatg
     9601 aagaggtgaa tatatgaaaa gaattataac tataaataca aaccaattaa ataagtccct
     9661 tgagactaaa gaactcttaa ctagaaaact tatcaatgct ggctttgaag tgtattcaga
     9721 tatttatcca gatacagagc ttattatatc aattggtggt gatggctctt ttttaagaac
     9781 agttagagat ttcgattttc ctgaaattcc cataatggga ataaatacag gtcatctggg
     9841 gttttttcct gatattttac cagataagat tgatagcttt attgaagctt atacaaaaaa
     9901 agattatatt attcaagaaa tgtcacttct taatgcggaa gtatatacaa ctactagtgg
     9961 ttctaatatg cttgctgtaa atgaggttgt tataagagga gacaagtcta ggactataca
    10021 cctaaattta agccttgata ataaacatat acaaaatttt agtggagatg gtatgattat
    10081 ttctacttcc acaggttcaa cagcctataa ctattctgct ggaggtagta ttgttgatat
    10141 aaatcttgaa cttatgcaga ttacaccttt acatccgata aataccaatg catataggtg
    10201 ctttacatca agtataatat gttcaaatga atctgttata aagattgcac cagaatatag
    10261 atttgaagat tctgtattga ttgtcgttga tggagttgag cacagattta gacaaatcga
    10321 aaatataaaa gttagtatat ctgatgcaaa aataaaatta cttagaatgt caaactatga
    10381 gttttggcat agagtatctg aaaaattttt atagactgta cttaggagga tttttaatat
    10441 gaaaatagga ataatgagtg atactcatgg tagcttactt tactttgaaa aagcgttaaa
    10501 tgtattatct gattgtgatg ttttattaca tggtggagat gtcttatatc atggacctag
    10561 aaatgatatt ccagaaggat ataatcctaa aaaatttatt gagacattaa ataaattaga
    10621 aaacattgtg attgtaaaag ggaactgtga tgcagatgtt gaccagatgg ttatagaaca
    10681 tcctattcag agtccttatg tcatgagcca atttggtgaa attagaatta tattgaatca
    10741 tggttatata gaatcagaag aagaaattat tgacaaagca aagaaaatgg gtggtgacat
    10801 attagtttta ggacatactc atgtaaaaaa actatacatg gatgataatt taatagtaat
    10861 aaatccagga agtacttcta tcccaaaaga tggaagccat tctgtcgcaa ttatagatat
    10921 cataaaaact gatgatgagg atgagcttga acttgatata aacttaattg acatcaatac
    10981 tggaaatata ataaatatca atgattagta aatttttaat aaaaataaaa taagctttaa
    11041 tttattaaat tcataataaa aacaagtaaa ccattttatt tctatctcaa ttataagttt
    11101 aagtacaata ccttaaactt ctaattaaat agaatctggt ttacttgttt tattatatgc
    11161 tatattgaaa tataacttag tttacttgtt tctattatat attatattgg agtataactt
    11221 agtttactta tttcattata tattatactg aaatataact tagtttactt attttattat
    11281 atatattgaa gtgcaacttg gtttacttgc tattattata tattaatttt gatttacaca
    11341 atattactgt aaggctaatg cttctgcata tggtttacta tttttacctc caccaacttg
    11401 tacaaattta tcagccttta ctttttttat aaaacttttc tgtgcactac ttaaacttcc
    11461 acttgcaagt actacaggtg actttgtttt tgatgctaat actccagcag caagcccatc
    11521 aactaaatca gcttctctac cacttccatc ttttgcaaaa tacatattat taaccttact
    11581 tgattttcca taaaactttt ctaaaacttt cgcattagta tcttttctat ctgtccctgc
    11641 tagtctttct gagtttacta actgtccttc aagtttttta cttatactgt actccccacc
    11701 tactatgtat gtcttagttg agttttgtgt aataaaattc tttgcctcac ttcttatgct
    11761 atccttacct gtatatatta tagctgtatt atcacttgca gctgggcttg atattgaagt
    11821 tgcatctgcc agtccattat acccattaac cactgccact tttgagacac ctaaatcacc
    11881 agtatcatct agctgtctag taagaagtaa agatgtttct tctctatctg ctccaccgat
    11941 tctactgact aatacttcca tatcttcaag agattctttt acacttgatg aaatagctcc
    12001 ttcacttcca attaaaataa cttctgctgg attaagcctt tttatttcct ctattacatt
    12061 atatggtata ctatttttat ttgtaagcag tattggtgca tccaatttac ttgccaaagg
    12121 agttgaagcc aatgcatctg gcaatgaatt tgagttaact agaaacaccc tttctgaacc
    12181 attattccaa ccactttgag atatttttat cgctgtatca aatctatctt caccaataag
    12241 tttttcctcc tttggagatt ttctaccttg aagataattg ttcatatcta cctgtgcatt
    12301 ttgattttta ctaatagtgt ctgcatatac acaacttggc aacataatcc caagtgaaag
    12361 tgcaaatgct attatttttt tagatttaat tttcaataca attccccctt ttaaatgtta
    12421 atacctattg atattcatac tttttattga taccctaaac taaaaataat tatcttttat
    12481 aaaaataatt acaaaaatag ctataccatt ttaaatacaa cacaagctta taatcagctt
    12541 gacttataaa gaatgatata gctattataa ctttaaatgc ttaattttta aatacctttt
    12601 aactaaactc tttcagcata tttttcagct attcttacaa ttagtttagt agctttctcc
    12661 atagaactaa ctggtataca ctcatttttc ccatggaagt ttaatccacc agtaaatata
    12721 tttggacaag gtaatccatt aaaagataat cttgctccat cagtaccacc tcttacagga
    12781 actaaacgtg gttttatacc taattcctcc atagcttctt tagctatatc aactataaac
    12841 ataactggct caatcttttc tttcatattg taataagagt cttttaaatc caattccact
    12901 ctattatcgt atttcttatt tattttttct atattctcta tcataataga ttttctattt
    12961 tcaaattttt ctttacaatg gtctctaata atataagcca tacttacatt ctcgacattt
    13021 ccacctatag aatttaaatg ccaaaatcct tcatatcctt ctgttgtttc tggtctttca
    13081 tctgctggga acatttctga tatttcagca gctatatgta tagcatttac cattttattt
    13141 ttagctgaac ctggatgaac atttctacca tgtatagtaa tagttgcatt tgcagcatta
    13201 aaattttcac attcaagttc tccaactatt cctccatcta aagtatatgc atattttgct
    13261 ccaaattttt caacatcaaa taaatcagca cctctaccaa tttcttcatc tggtgtaaat
    13321 cctattttta tatctccatg cttaatttct ggatgattta ttagatactc tattgcagtc
    13381 actatctcag ctataccagc tttatcatca gagccaagta gagttgttcc atcagtgact
    13441 attaaatcgt cacctttaaa ttcttccaac tctggagaat cctttacata cgtaataaca
    13501 tttaacgctt catttaaaac tatgtctttt ccgtcataat tttctattat tcttggttta
    13561 atatcttttc cactaacatc ttcaattgta tctaaatgtg atatgaatcc tatagtatcc
    13621 acaccatcag tatttccttt taaagtagcc attatataac tattttcatc taaactcaca
    13681 tcatcaacac ccattgtttt tagctcttca actatatact tagctaaaac tctttgacct
    13741 tctgaagatg gacagttctc acactgagaa ttagatgttg tatcaaatga tacatatttt
    13801 aaaaatcttt ccactacttt ttgtttcacg tatataccct ctttcttaat aagattaata
    13861 tttttttatt tacctgtgaa taaaatttta tttttaaaaa aatctatcaa ttattataat
    13921 taaaaaatat ccaccttctt aagtatatca tgcatacata ccaaacccaa acaaaaaatt
    13981 actccaatta ttccttttac ttggattcca ataaaaatcg ctgctagggt agctaatgga
    14041 tttagtccaa catttactga tactagttta ggttcaagta tttctctaat caaagacaaa
    14101 acaaaaaata tcacagtcat acttatagct ataaaataat tttttataag aaaataataa
    14161 acaattactg gaagatatat tgttattata cctaggaatg gaattaaatc cattaaagct
    14221 cctacaaacc ctactattag accgtatttc aatccaaata aagcaaaact tccccagatt
    14281 gcaaaaaatg ttatagccat aagtattgta taggccctta tatatccaac aagtgaaagc
    14341 cctgcctctt tctttacgtt ttttaccttc tttctaacat caacagtaaa catattataa
    14401 aaactatttt caatcttatc aaggtctttt gctataaagt acgtagcaat aaacaaggta
    14461 attataaata ttataatata tggaatagaa gtagccaatc caagtatact agtaagaaga
    14521 ttttttgtaa tttgcattac ttcaccactg aatttgttta ctaattgttc tacatcaaaa
    14581 ttggctattt cttgaaatga tttcataaag tcatttattt tatttaaaaa atacataata
    14641 gtattactta tatcctcagg attagcagat atattattta aaaatcccat taattctttc
    14701 attgaattta ttactattat tgtagtaact gtactgacta tagccactgc aagaaaactt
    14761 agaacaagag tagagatacc tttatttata cgaaacttat tttctaactt ttgtgatata
    14821 ggattaatca taagtgctat tatagcacct agaaaaaatg gagctatgta tggaaacgct
    14881 ttgtaaataa aaataaataa caatgtatat atacaaaaaa agatagtgtt gttttttaat
    14941 ttgtataaaa aattattttc tagtatgctc atcttaaaaa tctcctttct tctaatttat
    15001 atttttattt tgtccatttc atactagttt tatttgaata caaaataaaa agaagtgtat
    15061 aaatataaaa gatatctttt atatttatac acttctaata aatttaagaa cattattaca
    15121 ttatgtatat attcttattt ttttaattct atttttttct atactttcta tacaaaatct
    15181 tatattatca acttcaatta cttcattttc ttctggcaat ctgcttaggt gtccaattat
    15241 aaaacctcct atggaatcaa actcctcaga ttccaagttt acaccaataa gttcattaac
    15301 atcaccaatt ttagtacttc catctacaat atactcatct tcttttataa cttgtatttc
    15361 atcctcttct tcatcatatt catcctctat atctccaaca atgacttcta ctaaatcttc
    15421 tatagttaaa agtccagatg ttccaccata ctcatctaca actatagcca tctgactttt
    15481 ttcaagtttc atctcttcaa gaagttgtgt tatctttttg aactcatacg tgaaaaaagg
    15541 ttctctcata taatttttaa tatcaaatga ctctatttct tcatcagata aaaatataat
    15601 atcttttata ttaagtattc ctacaatatc atctatagtt tcttcataca caggcattct
    15661 acttaatttt tcagttttaa atacctgtat tatctcatca taactatctt ccatatctat
    15721 agctaccata tctattcttt gtaccattgc atttttagct tgcatatctc caaattcaaa
    15781 aacattattt atgatttctc tctcttccat ttcaaggacg ccttcttcat gacttacatt
    15841 taccatagtt tttagctctt cttcagttat gaatgattta gctcctttat ttgttatacc
    15901 aaataattta aatataatat ttgtaattat attaaatatc catactattg gtcttaatat
    15961 aaatataatt gcttttatag gtttagatac taatagagaa accttttctg aattatttgc
    16021 tgctattgtt tttggagtaa tctcaccaaa tattaatact aatactgtca taacagctgt
    16081 agcaagtgcc actccttttt ctcccattaa tcctataaac aaagatgttg atatggatgt
    16141 tgctgctata tttacgacgt tatttccaac caatatagaa cttaaaagct tatttggatt
    16201 ttctattaat gaacttacta acttagctcc tttaactcct tcatcttgca tatatctgat
    16261 tctgatttta ctcaacgaca ttaaggccgt ttcagatgct gagaaaaatg cagaccctat
    16321 aagtaatact attaaaaaaa taatctgaat caaattattg ggcgattcca acacttacca
    16381 cactcctact tacatgtttt tatattaata ttaattataa ataactttaa tattagtttt
    16441 tattatatca caattacata ttatttggaa atattagata atatattcaa ttaacatata
    16501 ttttctaact atattcaatt tttacttttg aacctattcc agcctcacta gatgaaacta
    16561 atagcttaac aggaactcta tttttaagtt cctcgacatg acttattata cccacactta
    16621 gattattact atgcaatctt tccaatgatt ctataacaat ttctaaaagt tcactatcta
    16681 atgaaccaaa accttcatct aaaaagaaaa attctaatgg tgcacttccc tttaattgta
    16741 tttgagatga aagagcaagt gctaatgata gagatgtcaa gaatgtctct ccaccagata
    16801 gagtatcaat gcttcttctt tctcctccat taaagttatc tctcattaca aagtttagtg
    16861 ttgagtcaat ttcaagtgca tatcttcctt ttgttattcc atccaatcgt tttgatgctt
    16921 ccaacgctat atattttaat tggtttgtag aaacatattc tacaaattta tttccttgta
    16981 cacatttatc taattcttcc aataagtcca cattgtgttc aactacctta agttgtttat
    17041 taaaatcatt tatcttatct aaagagtctt ttaatgttat taactgattt tgattagctc
    17101 caatatcttt ggatatattt cctatttcta ctttcaaatt ataaatttca tcttttaact
    17161 gttcaaagta ctccttcttt atgcttcttc catttaattt ttcttttgat gattttattt
    17221 taaatgataa taacttttgt tcttcttcat gctctagtat ttcttcaatc aattttttta
    17281 ctgcatcagt atctaataaa gctcttttta ctgcatatat actttcaaat ttattttctg
    17341 ctaatagttt atttagtgtt gatttttggt ctttatattg ttccttagca gttttaagtc
    17401 taccatctat attgtgcttt tcagcaatgt ttttttcata ttctaatctt tgctcttcca
    17461 attttttctt acttgattct tcttgttcta atatcttaca tatattttct tcaacattat
    17521 gtaaaagatt tttagctaaa tcacctttag ttatgctaat aacttcttta tacttattat
    17581 ccctactcaa tttcttttct acatataatt ctctagcctt tattaattct atctctattt
    17641 catgtagttt actctcattt tctcttattt taatatctag tgagtctcta ttttttaata
    17701 aatttgaatg cccagtactt agtaattcta attctttttc attctctctt atttctttta
    17761 cttttgacaa tagattagag actttagtaa tcgtctttaa tcctaggtat tcctgtttaa
    17821 gttttttaca tttattttct aaaccttcta tttctatgtt taaatctctt gttaatttct
    17881 tataattatt cagactctct cttattttta attcttcttt ttcaatttta tttttttctt
    17941 ctttcgctaa tgttacttta ttttcagtac tctctttttc tttctcccac tcctgaatat
    18001 tagattttaa caatgcaagt tttctttgtt cttcatcaag tttttttaat aattgactag
    18061 atggaatccc ccctagtttt ccttttacat cttcaagttc tttagtcttc attttttcta
    18121 tagataaata cccacttact ttagcattta attcctctat attgtttctt atacttattt
    18181 tttctttttc taatttctcc agtttttctt tgacaaaaga tatattttca tcatatttag
    18241 ttatattatg attttcatga tgttttgacc cacaaactgg acaaggcata ttttctttta
    18301 attcacgtct aagttcagag gctaaattta aatactttag ttcttctaat tctttttcta
    18361 ggtcatctct gttctttcta ttattttcaa gtttttcatt tagtaaattt acttcccttt
    18421 cagtattaaa cttactttca agtattatct ttaattcatc ttgtatactg cttttcttaa
    18481 tttcattttc tttagtatta tttgctttat ttcttaattc agtaacatat tcagatttta
    18541 aaagtaaatc agcactctta ccaggacatt ttttaagaag aacatccaag tgaagtgata
    18601 aattttctaa attacgattt acatcattct tatctttatc aatatatcta acttttaaat
    18661 ttatattttc tgtctctttg agtatttctt ctagtttatt tagcttttga tttttttctt
    18721 ccaatacgtt gttgtaatct ttctcgtatt catatgctag aaatattttt tgttttaatt
    18781 ctgcacttat atttacttta tcaatctttc cttcaacttc ttttatagat ttagtaactg
    18841 catcttttct ggactctgac acctgcttta ctttttctaa ttcaacttta gtcttattta
    18901 aattaattcc actttctttt agctctttta actctctatc taataaaact aactcttctt
    18961 ctaattttat tgcttgttgc aatcttattt tttcttcact caatttaggg atttcttcat
    19021 tcttaatttt gtttattttt ttatatctat tttttgtaac ttcaagctcc tgatttaata
    19081 ttgctaattt cttttctaaa ttacttactg cgaagctatc ttcatctatc tttttttcta
    19141 gattttgaac tgaacttatg tatgggtcta cctttttagc gttactatca ttttctacaa
    19201 gagcaatctt ttcttttatc tcactatttt ttaaatcaag ctcatttttt ctaagttcat
    19261 ttttttctaa ctttaattgt tcttcatata ctgttttact ttcttcataa gatttttgag
    19321 ctaggtctaa agcattattc ttatcttttt ccaaattttt taactctatt aattcttggt
    19381 ttatattgtt atatacttcc tctgtaattc catcatattg acttaatttt gatttcaaat
    19441 cttttaaatt ttgtaactgt atattttttc tctttttcac tttttcgcca agacttcttc
    19501 catatttctc aagattaaaa attctctcta acatgtctct tctatctgca cgtgataatc
    19561 ttagaaactc attaaatttg tcttgtggta aaactactga cctagtaaaa tcatttgccg
    19621 ttaatcctac gacctgcgct actttttcat ttacttccac aactttatca gctaaaacat
    19681 tttgggtatt gtcattcaat acttctatta atctagcatt agaggtcttt gttcctgttt
    19741 tgcttctagc aatggttcta tctacaatgt atcttcttct agtgtttttg cttcctatct
    19801 caaactcgta tgatataatt gccttatcgc acacactgtt tatatattct tttgtatttc
    19861 tggatatatt tccatacata gcaatagtta ttgcatctaa gattgtagat tttccacttc
    19921 cagtagttcc aaatatccca aacaaacctc tttcaattaa tttttcaaag tctataactt
    19981 gtttatcaat atagctatta agccctgtaa gttctaatct aattggtctc atctgctgat
    20041 tcaccttctt cacttattat atctagaaat aaatccataa gttctccttt tggctctaca
    20101 cctttactaa aagaatagaa ctctctaaac aactctgcca tagacttttc ttttatatct
    20161 acactttctt gttcataaca agatgttatt attggtttta tttctattat gtcttttaat
    20221 agttccttca tttttttaat ttcattctgt gatattatct catcagtatt tatttcaaaa
    20281 tatgaccaaa tctctctatc ttgattttgc tcacatatat ctagtgcttc ttctattcca
    20341 ttacatttaa atacttctat aggtttgtaa ttattaaaat atacatcttc aattattggt
    20401 ttttcaccag cttttatatc cactatgtat gcccctttgg tatacgctct ttcatccttg
    20461 ctatattgaa gtggagaacc agaatagtat gcatttaaac gttcggaagc tttttgttgt
    20521 ttatgtaaat gacctagtgc agtatattga gccttttctg gcaaatcttt tctttcaact
    20581 aaaaaacttc cacctaattg tataggtctt tctgattcag tactctctcc acctattaca
    20641 aatatatgtg atacggctat atttatagta tcacttctaa aattttcttc taaacttcta
    20701 aatatatctc ctaccttttc agaataattt ttttgtttct caaactcatc ttcacttgaa
    20761 aaaacttcat ttaatctttt ttcacttgga taaggtaatg tagcaaccac aatattttct
    20821 ccatttatat tcagttttgt acatccctga gttgcatatg ttatttcaaa ccctttatac
    20881 ttagcttcta tcgtagcact taatggataa ccatatataa gtattccctg ttcatgtgct
    20941 agaggtgtta ttgcagagag tctctctgga ttatcatgat ttcctgaaat tattaataca
    21001 cacctctgac cattattggc aagtcttgat acagtctgat aaaatagttt ttctgcttgt
    21061 gctggtggat tagaagtatc atatacatct ccagcaatta taaccatatc tatttcattt
    21121 gattctacta tttttataaa ttcctcacaa aactttgctt gttcctcaat tctagaatgg
    21181 ccttctaggg acttccctag atgccaatct gatgtatgaa taaatctcat tttttcccct
    21241 tcctctatct tttaggttta atttatatat aaaaactaat aacaaacagc ttcatcaact
    21301 ccaaataaat atatacattt ctcttttact gacttaccag ttaaagtttc aataatatct
    21361 gcatataaat ccaactgttt tttatatttc tctattattt gatttatatt ttcctcattt
    21421 acaaaatctg ttttataatc caccaaaact atttgattat cttcttcaaa ataagcatca
    21481 actatacctc ttaacatgac actttcatta tcataaagtt ttttatcatc attgtttata
    21541 agcttttcat aaatatatat gtctttcata tttacttgtg cataaattga tttttctcta
    21601 tttataattt cagcattcaa cattcttttt cctatgttag aggcaaaaaa tttatatatt
    21661 ttatatggat ttactatact agcttgtttt tctgttatta taccttttga aacaaaacct
    21721 cttatttgtg attttatatc atttacacta cttacatttt ttaagtctaa aacctccata
    21781 actaaatgga ctattgtacc tctttctgtt ccacttattt tttctctttc ttcttcattt
    21841 tgaataaata gaggcttttt taatattact ttttgttcaa atatagtatt tattaactct
    21901 tcttcataat tattttgtat cttttttatt tctgtaacag atatagtagc aggttttctt
    21961 gtactaaatt catatggata tatatagttt aatttttctt ctatttcact gtaataatca
    22021 ctgtcaggat tatctacatc tattttttca agaatttctt caatactttc tttttcttca
    22081 tcactcttct tttccactaa aatatcattc ttattccaca actttccata ccattttgaa
    22141 ttatgttcaa cattaaaaac tgcgtctaat cctacttctt ctagtagatt agataaatcc
    22201 ctatgtctta atacacaagg cattatccaa tctaaaaaat ttttaccttt taatatctcg
    22261 tattgtgata ttgtatctaa actttcaatt ccatttgacc atctctttat actatcttgt
    22321 atatttctag tagaaccagt tataatcaat ttttctttag ctcttgtaaa tgcaacatat
    22381 aaaactctca tctcttctga taaattttct atatttattt tgctttttaa tgcttctttg
    22441 gctatacttg gaaaagaaat ccttctctca tagtctacaa attgaggtcc ataccctaaa
    22501 ttatgatgat ataaaatact ctttttaaaa tcttgagtat tgaaattttt acccattgca
    22561 gagcatatga ctactggaaa ttctaaacct ttacttttat gaatactcat tattctaaca
    22621 acatttgcat tctcaccaag tgtttttgca cttcccatat cagaactaga tttttttaat
    22681 ttctctataa aatttacaaa gttgaatatt cctttaagac tggtttcttc aaattgtttt
    22741 gctctctcaa acaatacttt taaattagct tgtctttgac ttccacctgg caatgctcca
    22801 acataggcat agtatccagt ccttgtgtac aagtaccata taaattcatc tgtactcatg
    22861 tacattgact tttctttaaa ttcctttagc ttaattaaaa aatctttact tttgtttatg
    22921 cactcttcac ttggtataaa ctcagactca ttttcatttt gagaatctgt aaatccatca
    22981 tactctgctg tactttttaa aacctcataa aaaattttat ctttactctg aactctaata
    23041 tcaatcaaat cctctggtgt aaatccaaat ataggtgatt ttaatacaga tataagaggt
    23101 atatcttgca ttggattatc tattatttga agtaatgaca atattgtttt tatttcaatc
    23161 gtatcaaaat accccactcc aacatcagca tatgttggta tatccatatt catcaattca
    23221 tctgcaaaca ctggagccca tgcagatgta gctcttaaaa gaataactat atctctaaat
    23281 tccacaggtc tatatccatc tattccctta tcatagacct tttgaatttt tccatcttca
    23341 ttgactttca ttaaatcttt gataatgttt ccaaccattc tagcctcaag ttgtatatta
    23401 tctaattttt cttcttcctc tatttcattt tctttgttat taattctatc gtctttatca
    23461 tttataatat catcatcaag tttattatct ttttgtatta ggtgtatctc cgttgctcca
    23521 ccaataatag atttctcgtc tgtatccaca ttaaaatttg cacctaaatt tagtctttct
    23581 ttttctgtat attcaatttc tcctatattc tcattcatta tattttcaaa tatataattt
    23641 acagcatcta caacttcttc tctacttcta aaatttttgt aaagcattat ttttctatgg
    23701 gaacttccct ttttatcatt atagttatta tatttttgca aaaataattc tggctttgct
    23761 tgtctaaatc tatatatact ctgttttaca tcaccaacca taaatctatt tggagtttct
    23821 gtatttgcaa cagctttaag cagtacttcc tgcactagat tactatcctg atattcatct
    23881 ataaatattt cataaaactt gtttctatat ccaactgcta tatcagaagg tacaatatta
    23941 cctttttcat ctacatctgt aagtatattt aatgcaaaat gctctatatc gttaaaatct
    24001 attattcctt tttctctttt cttattgcta tattcctctt caaatctcag tacaacacta
    24061 gatattggtt taactatgtt ataaagatat tttatttctt ctctaataga atcattatct
    24121 ttattgaatg tagcacttac tatactttct aaagatttct tagtcttatc tcttatagtt
    24181 tttgcctttt cttttgattc ttttatatac gaaggagcat cttttgaaat tctttttact
    24241 ccttttacat aattttcaaa ggacatactt gccatctttt tatatgcctc atcccaagat
    24301 ttattacatg cttgtgaaat gtcagcaatt tttttatatt caacggataa cttttctgca
    24361 aaagtttcta attcttctat tgattccact tcttttaatg ccctttccat atttagacaa
    24421 agaccattaa tctctatttt tacagtatct aatatagctc tagcccaaat agactgtgaa
    24481 aaatcaaaat tttcatcaat attaaatcgt tctgctgagt caatcaacca ttttttaggt
    24541 tcaggagaag ccattgcaaa tgaatatatt cccaagataa tatcttgtac ttctttatct
    24601 cctcctcttt ctgcataact ttcaactaaa tttaaaaacc cctcatctct ttcttcatat
    24661 aaatcttcaa atacttcctc tattgcttct tgtttcaaaa tagcacattc agtttggtct
    24721 cctattctga aattagggtc taagtttatt ctatgaaagt ttgattttat aacatccaaa
    24781 cagaatgagt gtattgttgt aatactagat ttatttaata aaacaagttg gttttgtaaa
    24841 tgtttatttt ctgggttttc atctaaggct tttcctatag catcccctat cctctctctc
    24901 atttctgacg cagcagcatt agtaaatgta acaactaata acttatctat atcaattgga
    24961 ttttctctac ttgttatcat ttgaattata cgctcaacta gcacggctgt ttttcctgaa
    25021 ccagcagcag cagcaaccaa taaattgcat tctctacttt ctataacttc taattgctct
    25081 tttgtccatt taggagaact cactaattaa catctccttt catcattttt attatctctt
    25141 cattactctt attatttaaa tttttatact tgttatcttt tatagttgaa tcaaactgac
    25201 atatagcaga gtaatcacaa aagtcacatg atgttccatc tttatgttta tatggagcta
    25261 tacgtatttc tccacttaac atttcttcac ataaatcctt tatagcatgc cttacatatt
    25321 ttctaagcag tttgaaatcc tcatagctta ttgctgaagt acttttacct acatttccat
    25381 ccttagtaag acctactggt attactaaag agtttttcct ttctccatct attaatgatt
    25441 tatccatctc tttaactatg tgagagtctt ttactacaag ccccttcatt ctaagttctt
    25501 ttaatattgc ttcttgtatt tcttcatcat ctttatcttc attaaatttt gctattggat
    25561 tatttattct acaatatagt attgcagctg gatttatatt tatatcttca tcctttgcac
    25621 tttcaaggat tgcatctaaa tacacaagta attgtaattg tagaccataa tatatttctg
    25681 ttagacttat ggacttattg cctgacttat aatctattat tcttatatat ttattttctc
    25741 cttcttcaaa ttcatcaact ctatctattt gtccaattaa acttacttct tgtccatttt
    25801 ctaacaccat ttttataggt ggatattttc cattgttgcc aaatttaact tcataatcta
    25861 ttggttcaaa tgaaccttgt tttatttgct gactaattat agttatagca gttgtcaaca
    25921 tattctttaa tctatatgca aggtatttat acctctcaga actatttaat atatatcctg
    25981 gtatctttaa aattatttca tcaactatta tacctatctt taattctata tatttttcat
    26041 caatatctct ccaagttaaa ttatcttgta aaagttcttt tgaaaaccta tctaaaatat
    26101 tatgaataaa agttcctaaa tctggtggtg taaatgaata ctctttacgc ttttttgctt
    26161 ttagaccata ttgtataaaa taagcaaatg gacattcagc atatttttca agcctagata
    26221 tgctaagaga attactttta tacaatgacc ttattttttt ctcttcaatc ttatgaactt
    26281 gattagtata actaagacct tttataactt tttttgttat agaactatag atttcatctt
    26341 ttaaatagta cctatatata tctaaccaaa tactattaat ctcttctgta ttgtaaccat
    26401 cactgtcata attttttatt acgtttataa gttcattaaa tgtaggggat ttaaccgtta
    26461 tttttttaag tatatcttta tcagtgcttt tattttcttc cactaaataa cttttatttt
    26521 ctatatttgg aaatatcttc ttaagtcttg atataattat tgatggtcta agcgttttac
    26581 cttcatggtc tgatattgga tatgttatgg ttaaattctt agatgtcgaa gtaagagctt
    26641 tatatacaag aaactgttct tcaaaactcc tagtcttact atcaatatca acttcgattc
    26701 ctttgtttcc aagactttcc ctatcattat cactcaatat tccactatcc tttgtaatca
    26761 gcggaaacac accatcagta gttccaacta ggtataaata tttcgtgtct ggatttttca
    26821 ttctatccac actacttaca agtacctggt ctatactagg aggaaccagt cctaattcat
    26881 attcatcaaa acctaaggtt attaatttta taaatttttc taaagaaatt atttcatcac
    26941 ccataagttc caccatttgg tctaatatgt ccactactat gtcccaaact tgtgagtatt
    27001 gatttgcaac atcaagttcc cctttatcct taaaatttat tattaaactt tctatagttt
    27061 ctggcatatt tatatctaat aaaaattcat atatatatct acagatttct tttactctat
    27121 tttttcctct taatttttct tgtaaaatta ctataggctc taatactcta ttttttattt
    27181 catttatttt ctcttttaat tccagttcaa attcactttc ttgacctgaa agactttggg
    27241 ttattctata atcccatttt tcatcaaacc attttttacc tttaatccca ttagccaaca
    27301 cataattttc tagtaaattt atatcatcat tatcaattcc tataagtcca ctttttaaat
    27361 acctaaacat agtttcatac ccatatcgtc tattcttcat ttctaaagct gaaataatta
    27421 aaataactat tggattactt tttgcctctc tcttcttatc tataaagttt ggtatattat
    27481 attcattaaa tatagagtgt actaaaaaat catatctatt taaatctctt gtagcaatgg
    27541 ttatatctct atatcttaca ttcttatctc taactaaatg tacaatttct ctggctatct
    27601 cttccacctc tgaatataaa ttgttaaact cttttatctt tatattttca gttggatttg
    27661 aatatatttt atatggataa gcattataaa atctttctaa gtgctgtaat tccttaactt
    27721 tttcaaatct ttttataaca cctgtgttta aatttacttg tggcaatatt tttatgcctt
    27781 cttcgttgca caattgagtc aattttgaat aagtaaattt cgttctagaa aacatatcag
    27841 atttactgta cccaatatag ttgatatcat ctacagtcaa agagatatta acacttttag
    27901 acttatttaa tatactcttt atgacattgt actgatttgg tgtaaaacct gtaaattcat
    27961 ctatataaac acaagctcca tctaaataac tgcttaattc aattttactt gccaatgaag
    28021 ttaacatatc ttgagcatct acataatttt catgcaattt cccttcaaaa gaattatata
    28081 ttttacttat atcttttaat tttaaactta aagtttcatt atccaattct ccagatatat
    28141 tttctaacat ttcagggctt atattgtatt gtttcatttc tgatatcata tctgtaattg
    28201 agcttacaaa accagactgc gattttgatt ttgaaaacac atttaattct tcacttacat
    28261 cttcaatagc tctatatacc atcattgctt ttccactaga atttatattt acatctgtca
    28321 atcctcctac ttgagaaaaa actatattac ttagtgtctt aaagctaagt accctaactc
    28381 ttagatactt atctttttct tttcctaaga atagcttact tactctattt tcagcctcaa
    28441 atgtgtactg ttctggaact aaaagtataa ttgatgtcgt ttcatttttt tgagcttctt
    28501 ttttaatctc atctagtata taggtggatt ttccacttcc acttctacct aatacaaacc
    28561 taagtcccat aaatttccgc ctccaaaata ctataatcta tttctttgcc attacataac
    28621 ttattaaatt cacaatactt gcaatgcttt ttatttctat agcacatacg ttcttcttta
    28681 ttatatacct tttctatatc attgtataga ttgtcttcgt atgtatgttc ttcataatta
    28741 gtgtttttta tcatacttat ataattttga attttatttc tatttgcttc atgtttttct
    28801 tcatcatata aaatggtaat tggtaagtca tcaaattcag gttgataata tctcatattt
    28861 atatctttaa catttatgtc catttttaaa actttaggta ttacttcttt tgctaaaaac
    28921 atataaacaa tagtttgtat tctattttct acgtgtttat agtcaatctt tttagactcg
    28981 gttttccaat cccatatttc tatattgtta tttccaatta caactaaatc atactttgcc
    29041 tgtattatat ccccatttaa tgtcatcctt acttcatatt ctggcaaata cgtttttcct
    29101 ttttcaatag gaacaacttt ttttatattt tccatccact ttaaaaactt tttactattt
    29161 ttatcatcct cattaaaata gattccaaga ggtatattgc taaaatatct ttcacaaatc
    29221 aaatgaaaat ctcttcctgt ttttaaagtt tcataatact ctctactacc aacatcatca
    29281 tttttccaat ttattctatc aatatattta tatttaaatt tcaatggaca tgatttatat
    29341 gtgttaattg aattttgact ataagaaaaa tattttaatt tatcgttcac tcatatcact
    29401 tctctcttaa ctttattttt tagtagttac ttactgtaca tattgtttta tcccctttta
    29461 gatatatttc aagcaatctt ttaataactt attatatatt aatttgacct tttttcaata
    29521 atatgttgat taagtctgct taaatatata catggttttt gctcttttct cttattccct
    29581 atatcacttt catctctata agcacttcct gaaagtacta acatttcctt tgctctagta
    29641 atccctacat ataaaagtct aatcttttca tttatagaat caatcttcgt tttatgagca
    29701 taatctgttg aaatacttcc ttttaatata gcttcaactt ctgatttaat aattgccata
    29761 gggtttttat atttttcttt tagataccat ttatcactct gaaacttttg attaatattg
    29821 tcagggaaat tatattcaac aagacctagt aaaaacacac aatcccattc catccccttt
    29881 gatttatgat agttacatag tgttatacta ccgggctctg gttcatatcc atttatctca
    29941 tataccacat ctattatatg attaaacacc ctgttcttta catcaaacaa taaattatat
    30001 acatcttcta agtccatatt tatattttct gatactagat actttatata aaaagatata
    30061 taatccagta cagctttatc ttctttttct aaattaagtt ttttcccaat aaacaaaact
    30121 aagaggtcta accttattat tggatattct aatatctgtt ttataatatc tattcctctt
    30181 ttaaaactta aatatatgtc actttcttct ttaaattcta ttaaatcatt atctaactta
    30241 ctaaattcaa aattataaat aatttcttca gtagaatagt tttttaattc ttctaaaaaa
    30301 tactttattt cttcttcatt atcagtatct ataaaaactt tattaagtac aactattaac
    30361 ttttctacat catcacaatg taacataaaa tcaataatat atgctatgtt gtttataacc
    30421 ttccttttat tcaaagaatt tggtcctaat tcttcaaaag ttagattatt ctctctaagt
    30481 tctttagcaa cttgtgtcac ttgttcatta aatggaacca atatacctat acttttatct
    30541 ggatatttat tttttatacc tttaacatat ctagcagttt gctctatttc aattttccaa
    30601 ctctcatacc actttacatt tatattataa tgttctggat taggattttc tttatatccc
    30661 attccatttg gcacagtttt tatttgcata tcttcaagag catgtctaca ttctttctgt
    30721 ctaaattcct ttgtaacata cttgacaaga gtgtttgcta aatcaagtat atcttttgaa
    30781 cttcgatttg acatatccat cctataacag aaatctgcac tatttataaa ttctttaaaa
    30841 aatttagggt ctgatgatga aaatgttcct gttatactct gatttatatc tccaactcta
    30901 actaggtttt tattttcgct agatataatt tttattatat taccttgaat ttcatttgag
    30961 tcctgacact catcttcaaa tatatatttg tatctagttt gaaacttctt tctaagccct
    31021 tcgtctaagc ttaaagtttt ttctgctaaa attaatatat catcataatc tagcaatcca
    31081 ttttgcttta attttctatc atacaattta tatattggta aaataatttt aagcattcct
    31141 ttgtgtcctc ttgacaatat ttcttcaagt ttatctggag aaattccctt atacttaagt
    31201 tcacttattg catttcctgc aaattcaaaa aaacctcttt cccaggcttc aagagttatt
    31261 tctttccagc gttcatcttt ttgctccttt aaaaaccatc taaaagctct ttctccacct
    31321 tcaaatctaa atctacttat acattcattt aatataatac ttttttgcaa atcatcagct
    31381 atattaaaat cctcacttaa cattacaact tctggctttt cctttattat ttttacagcc
    31441 aaactgtgta tagtcattac ttcatacgaa ttagtatcat ctattttatt ctcttcaaga
    31501 atctttctta ttctaccttt aaaattattt acagcactat tcatatatgt aagaattaat
    31561 attttaccac ccttatgttt ctgttcaagt aatagtttag caactaaatt tgttattata
    31621 aatgtttttc cagcacctgg tactgctggt actgccatag taccattatc gtaatttatt
    31681 attggtatct ggtcttctct ataatttata ttaatcataa tttttatctc ctttatgtga
    31741 gatttttaaa agtatactat ataatatact atcttggata taaccattaa tcgcatattc
    31801 acttttaaat gcatagattt tctttgcatt taataacaaa ttatatactg tattgtatag
    31861 atagtatttt ttataatatt cttctatttc atttgtataa atcttttttt cttcaaaaga
    31921 ttttctaagt acaattaaat tacttatatc tttttctatt ttcatattcc aagcattact
    31981 tcctatatct acccatattt gtatacttct atctatattt gatgatatat atgaataagg
    32041 agtagttatt actattttgt ctttggattt caactcttct atatcgacaa cagagtaata
    32101 atcgtttatt gtacttttta aagcttctac aaatatcttt tcttttttca tcccaagtag
    32161 acttaaactt tcactaaaag actcactttc agctattatc tttttacata acccaacatt
    32221 ctttttacct tcttttaaat ttaacatttt atctatgtaa aattgagtta aaaattcatg
    32281 tatttttata tcactcttct ttttttcttc aatatactgt aaaatatcac gatactgact
    32341 atgttcatct atattatcaa tacttaaatg tcttgtttca ttaaaaatct taaatgcttt
    32401 tatcctattt atttctaaaa gagtttctaa aaagcttaag tattcatcat cttttataaa
    32461 atctaaatat ccatagaata tacatgtagc cactaccaat gcattcccat atggataatc
    32521 cacagctttt ttatccttct tagtactaaa aacatctata tttttttcta tcaaagaatc
    32581 tctaatttga tgctccaata tactattgtt tataggagat ataattgcta tatctcttgg
    32641 agatacatct tcttctataa gagatataac tttttcactt attaaatcta acatttcatt
    32701 atataattga cttgattgat ttaattctat atttgcaggt aataagtata aatcttctac
    32761 tctaatctcc ttctttttaa tattttcttc tttaggctta atttttgata ttattttctc
    32821 atttatatag tctatatcca cattattaaa tacagaataa tccctactat aatcaaaata
    32881 tatataactt tgtaatgtat tatccaatgc ttctgttata aaatcaactt ctgcattact
    32941 tgagctttca aggctatcca caattatata tctcacttct gtaaataagt tttttaaata
    33001 aaaatcatca ttaagtaggt attgattgta taaatatatg cttagtgcat tatctaaaat
    33061 tgaatttgat agtaaggaat ttatataata tgatataatt tcatccattt gagtgtatga
    33121 aaatctatat atactatctt tatttttctt tgatgaatat attttttctc caataaaatc
    33181 aaaatcaatt aaattaaatg cagacttatt tatgttagtc attatactat ttgctatgga
    33241 tttattggtt cctgtaatat cttcaaaata tccttcttgt atcctttttt ccttaacttt
    33301 ttgcactatt aaatattcac ttaaactatt tgatataaat gttggagata caacctttgt
    33361 acttatttca tcacattttt ctattattag tggccagtat ttaactaatt ctttcttcac
    33421 aaaacttata tatgttgtta ttttcagttc ttcactataa tttaatctta gagccctttc
    33481 atatcgtatc tttgtgttat tgtttggtac taacaaaaga atacaccttg aattattacc
    33541 agttttatct aataattctc tatatttatc taaagcttca tttccatatt cttttaaact
    33601 acttttttca aataaatatt ccatataatc accttcctca attaaattaa gtgtatatta
    33661 tctaattata tcatattgtg tattttacaa aacacaatca aattattgca tttaactaca
    33721 aaaaaagact gtatatatac ttgcattcac atgtgcaaat gtacatacag tcttactatt
    33781 gatacactta tatttcattt tttgacttta gtataaattt acattaagtt tttaaatatt
    33841 tttatttcca tttattttaa tgaatcttct attcctttac ctatttgtgt acccatttct
    33901 tttacttttg cagatgagtt tattaaactc atatcatttt cattatccaa aaatgcacat
    33961 tctacaagta cagctggcat atcattactt cttaaaacgt ataaataatc ttttgtagaa
    34021 tcactaggaa gtactcttgt ttttatacct ctatttgtaa gtttaaattt ctctaaaatt
    34081 ctatttaata tattggtagc tacagtttta gtagtccctc catttttatc tttaagctta
    34141 taaaatactt ctacaccatg accttgttta ttagttgagc cattataatg tatacttgta
    34201 aataaatctg gtttcaatga attagataga gcagttctat ttccaagaga taaagtctta
    34261 tctgtgtctc ttgtcattat tacattgaat ccctttgaac gtaaatattc agttgttgca
    34321 agtgaagtat ttaaattgta atctttttca atcattcctc cagatgaatt tccaggtgct
    34381 gaaccaccat gccctgggtc aatcataact gtcttgcccc cactatttcc tggttctgtt
    34441 ggagtcgtat tgtgtttaga taatgatgat gctatagagc tcattacctt agaagtcaat
    34501 cctcctccta ttttgtatac cttattagct gatttagctt ctaaattatc tttatggtat
    34561 gcagatacat acgtctttgg tgttataagt attggagatt ttaagttcgc agccaatgga
    34621 cctgctgcta aagcatcaac aagtacatct gatttagcta ctaaaacagc ctctaactca
    34681 ttatctgtat agaatttttt tattacgttt gcatttgttt cgtgtctatc tgctccgtat
    34741 actctattat tagtaacact atctttagtt attccattta ccttatttat aacatttgtt
    34801 gatatcattt gaggaccacc tataaaataa gcattttgta aatcctcact ctttaaccat
    34861 ttatatgtat tgtctgtaat actatcttta tcagttaata taattggttg cttgtcttga
    34921 cctgcttttg ctgctatagt aagtgcatcc acttctccgc cattagcatt tgttatgtat
    34981 attttttcca catcatgatt tttatctatt tcctttgcta tcaataaaga tgtctcatat
    35041 ctattagaac catttaaacg tgtttgacta gcatttacag ttgatttaat ttgattagca
    35101 gtagttttag aaatagcatt ctcatctcca attataatta tatctttagg gtttaggcgc
    35161 tttaattctg attttacact attaggtaca ttgttttttt caaccaaaag tattggtgca
    35221 ttatatgtgg ttgccagtgg agttgatata atgccatcta tacttacatc cccatttatt
    35281 ataactacct tatctgaacc atttttccag ccctctttac ttattttaac tgctgtttca
    35341 tatctatcac ttcctgtaag ttctgtaata ggtgaagaag tttttctcaa atatttattt
    35401 acatcttttc tagaagttat atcttcaatt atacattcct gtgttacatt atagtctttt
    35461 aaatttaatc ccagcttttc taggtcttgt ctgtttaaag ttttaggatt atttttgctg
    35521 tttattgtta tcccttgtag ttcccaaaca tttagatatt ctgggtcttg gttataattg
    35581 atagttcctt catatgcccc agttgattga ttgtaattta gagttgtatt caatggcatg
    35641 tcatagcatt tatactttaa tgtaatgcta tctgcatctg gtttttcttt aaatttgaca
    35701 ct
//
LOCUS       DAMJYC010000034        35637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJYC010000034 DAMJYC010000000
VERSION     DAMJYC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.035"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            12..1868
                     /locus_tag="PC472_001820"
     CDS             12..1868
                     /locus_tag="PC472_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2727146.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1951..2191
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2257..2492
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2577..3794
                     /locus_tag="PC472_001821"
     CDS             2577..3794
                     /locus_tag="PC472_001821"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2727147.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3911..4393
                     /gene="cdeM"
                     /locus_tag="PC472_001822"
     CDS             3911..4393
                     /gene="cdeM"
                     /locus_tag="PC472_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2727148.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4658..5923
                     /gene="hisD"
                     /locus_tag="PC472_001823"
     CDS             4658..5923
                     /gene="hisD"
                     /locus_tag="PC472_001823"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2727149.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6126..6878)
                     /locus_tag="PC472_001824"
     CDS             complement(6126..6878)
                     /locus_tag="PC472_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2727150.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(6996..7265)
                     /locus_tag="PC472_001825"
     CDS             complement(6996..7265)
                     /locus_tag="PC472_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727151.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7740..8834
                     /locus_tag="PC472_001826"
     CDS             7740..8834
                     /locus_tag="PC472_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2727152.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8836..9786
                     /locus_tag="PC472_001827"
     CDS             8836..9786
                     /locus_tag="PC472_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2727153.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     RGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVNHG"
     gene            9779..10963
                     /locus_tag="PC472_001828"
     CDS             9779..10963
                     /locus_tag="PC472_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727154.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DVYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10976..12472
                     /locus_tag="PC472_001829"
     CDS             10976..12472
                     /locus_tag="PC472_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2727155.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12495..13445
                     /locus_tag="PC472_001830"
     CDS             12495..13445
                     /locus_tag="PC472_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727156.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFS
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13466..14428
                     /locus_tag="PC472_001831"
     CDS             13466..14428
                     /locus_tag="PC472_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2727157.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14800..15432
                     /locus_tag="PC472_001832"
     CDS             14800..15432
                     /locus_tag="PC472_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2727158.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15843..15935
                     /gene="kdpF"
                     /locus_tag="PC472_001833"
     CDS             15843..15935
                     /gene="kdpF"
                     /locus_tag="PC472_001833"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2727159.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15976..17658
                     /gene="kdpA"
                     /locus_tag="PC472_001834"
     CDS             15976..17658
                     /gene="kdpA"
                     /locus_tag="PC472_001834"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2727160.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17683..19740
                     /gene="kdpB"
                     /locus_tag="PC472_001835"
     CDS             17683..19740
                     /gene="kdpB"
                     /locus_tag="PC472_001835"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2727161.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19759..20385
                     /locus_tag="PC472_001836"
     CDS             19759..20385
                     /locus_tag="PC472_001836"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2727162.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20622..21530
                     /gene="cysK"
                     /locus_tag="PC472_001837"
     CDS             20622..21530
                     /gene="cysK"
                     /locus_tag="PC472_001837"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2727163.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21557..22147
                     /gene="cysE"
                     /locus_tag="PC472_001838"
     CDS             21557..22147
                     /gene="cysE"
                     /locus_tag="PC472_001838"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2727164.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22307..22495
                     /locus_tag="PC472_001839"
     CDS             22307..22495
                     /locus_tag="PC472_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2727165.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22549..23418)
                     /locus_tag="PC472_001840"
     CDS             complement(22549..23418)
                     /locus_tag="PC472_001840"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2727166.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23615..24859)
                     /locus_tag="PC472_001841"
     CDS             complement(23615..24859)
                     /locus_tag="PC472_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727167.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINSKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25150..25461
                     /locus_tag="PC472_001842"
     CDS             25150..25461
                     /locus_tag="PC472_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2727168.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      25554..25646
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25746..26540
                     /gene="thiD"
                     /locus_tag="PC472_001843"
     CDS             25746..26540
                     /gene="thiD"
                     /locus_tag="PC472_001843"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2727169.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26564..27352
                     /gene="thiM"
                     /locus_tag="PC472_001844"
     CDS             26564..27352
                     /gene="thiM"
                     /locus_tag="PC472_001844"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2727170.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            27375..28007
                     /gene="thiE"
                     /locus_tag="PC472_001845"
     CDS             27375..28007
                     /gene="thiE"
                     /locus_tag="PC472_001845"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2727171.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSVSFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28146..28448
                     /locus_tag="PC472_001846"
     CDS             28146..28448
                     /locus_tag="PC472_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727172.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28514..29263)
                     /locus_tag="PC472_001847"
     CDS             complement(28514..29263)
                     /locus_tag="PC472_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727173.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(29257..30219)
                     /locus_tag="PC472_001848"
     CDS             complement(29257..30219)
                     /locus_tag="PC472_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727174.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(30223..30681)
                     /locus_tag="PC472_001849"
     CDS             complement(30223..30681)
                     /locus_tag="PC472_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2727175.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31036..31392
                     /locus_tag="PC472_001850"
     CDS             31036..31392
                     /locus_tag="PC472_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727176.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31464..32180
                     /locus_tag="PC472_001851"
     CDS             31464..32180
                     /locus_tag="PC472_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727177.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32173..32997
                     /locus_tag="PC472_001852"
     CDS             32173..32997
                     /locus_tag="PC472_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727178.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            33009..34208
                     /locus_tag="PC472_001853"
     CDS             33009..34208
                     /locus_tag="PC472_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727179.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            34217..35029
                     /locus_tag="PC472_001854"
     CDS             34217..35029
                     /locus_tag="PC472_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727180.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYSFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            35040..>35637
                     /locus_tag="PC472_001855"
     CDS             35040..>35637
                     /locus_tag="PC472_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727181.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKID"
BASE COUNT        13846 a         3809 c         6206 g        11776 t
ORIGIN      
        1 gtggttagaa tatggatacc cataataaat atgtaaattt tataaaaaat atacctgtac
       61 cttttttata ttgtagaatt gtaaagagac aagaagatat agaatatcga gtagaatata
      121 taagtaaggg tatgggcaaa gtattacaat tagaagaagg tatttgtgat aaaaatatac
      181 tagatgtatt gcctgtattt aagtctaaaa aatatttcaa agagttgttc tcaaatgaag
      241 tggattgtat aaaaaggtac attccgacac ttaaaaactg gataaatata aaaaaacaga
      301 taatagggga ttcatacata attttgtatt ttggtaaaat tgtatttgat tatagacaga
      361 taatcgattc ctttgataaa aaggaaaaag tagcttatat taaagacgaa gaaggcatat
      421 atatagattg cagtgaaaat ttaataccaa tattaaataa caacattaaa acaacgaaag
      481 atatctttgg taaaaatgat atagaagtat ggggagagaa tacaggaaaa ttatttagag
      541 atgattatag agaaggagta tctagtaaaa aaagattttt acaaaatctt tttgaatatg
      601 aagaaacatt ttttatggtt gaaaaatatt ttttgtatga tgaagacgaa cttttaggta
      661 ctatagggat agtagacaat ataatatata gtggttacag taatagaaat tataactcaa
      721 aagatttaat gaagatgata gaacactcaa ttccagagaa tatgttttat aaagatgtgt
      781 atggaaatta tattggattt aattcaggtt ttttgaattt agcttgtatg aataaagaag
      841 aattattagg gaagaatagt tataaaatat cagaagaaga agctttaata gataagatat
      901 ttgaaagtga taagggtgta gttgaaaata aaaaagtagt tacatttgag ttaaatataa
      961 gtatgaatga tgaaaacaag tgcattgaga ttacaaaaag gccgtttttt gatagctatg
     1021 gaagtgttat tggaataatt ggaacagcaa gggatataag tagaagaaaa agattagaag
     1081 aggaaatgga taaaactagg atggaatttt ttgcaaattt atcccatgaa cttagaacgc
     1141 ctataaactt aatatcatct tcattacaag tgatagaaaa aaaagaagca gatttaatag
     1201 aatcaaatga tactttaaaa agaaatttag gtataataaa acaaaatgga aatagaatac
     1261 tgaggttagt aaataatgtt atagatttta caaaaatgca atcaggatat ttagatttta
     1321 aaccagaaga atctgatata attgcattta tagaggagat atgtatgtct gtagctgatt
     1381 ttgcaagcca aaataatata caacttacat ttgatacaga aatagaagaa ttttcaatgt
     1441 tatttgattc agaaaaattg gaaagaataa tattaaattt actttctaat ggaattaagt
     1501 acaataagaa agatggaaaa ataaatatat ttttatatgt gaaagataat gtatttaata
     1561 tgaaaatatc tgatagtgga ataggaatac caaaagaaaa aatagataaa atatttaata
     1621 ggtttgaaca gattgacaat gaactttctt acagagttaa aggaagtggt attggacttt
     1681 cattagtgaa atctttggtt gagttgcatg agggaagtat atctttaaaa agtcaacttg
     1741 gtattggaag tgaatttata gtttcattgc ctgttagaag taaaaataat attgaaaaat
     1801 ataatcataa aagagaaatc agcaatgaac taagtaaaaa attagaaata gagttttcgg
     1861 atttataagt ttaaatataa aaaataaaat tttaaaaaag tattgacaat tgatttagaa
     1921 gggtttaaaa taaattacaa cataataaat aaaaaggcga tgaataggaa gagtaaatct
     1981 aattcgatta ccagagagaa gagtccgttg gctggaagac tttttaaatt agagggattg
     2041 aaaactacct aggagctact ttctgaagtc atctgcatta gtagatttta gtaagaatag
     2101 tcgttagctg cgttaaagtg taaagtgagt tttcatgaca tatgaaaact aaattaggtg
     2161 gaaccgcggg aataatactc tcgtccttat gttataagga tgagagtttt ttgttattta
     2221 aaaatataaa cagtaatcat aataattata gaaaatataa aagcaatgaa taggaagagt
     2281 aaatttaatt cgactatcag agaggaaagt tcacaggctg aaagactttc taagtttgag
     2341 aaattgaaaa ctacctagga gctgtggtct gaagttactt taaaaagttt tagtaagact
     2401 aaccgttaac tgcgttaaag tgtaaagtgg attttatcaa taaatgataa aatcaaattg
     2461 ggtggaaccg cgagtataga ctcgtccctt tatataggga tgagtctatt tttattgcaa
     2521 taaaagattg aaaataaaaa aaaatcggaa aattataaat ttaggaggaa aataaaatgg
     2581 agaataaagt gaaaattgga gtactaggat atggagttgt aggaagtgga cttatagata
     2641 taatagataa taataaagaa aaaagaaata tagaaatagt tggaatttta gttaataatt
     2701 tggaaaaaca taaggacaaa aaatattcta atataataac taataatata gatgatatat
     2761 ttaataaaga tatagatata cttgttgaag tcatgggtgg tttagaacca tcattatctt
     2821 atataaaaaa agcattaaac aataaaattc atgttgttac agcaaataaa gatttgttag
     2881 ctgaatgtgg agatgagctt gcaaaattag caagtgaaaa taaagtaagt attaaatttg
     2941 aagcttcagt tgcaggaggt ataccagtat taaaaccaat aatagaatca ctagaaggaa
     3001 ataatataga tagtataaat gcaattttaa atggaactac taattttata ttatctaaaa
     3061 tgtatgatga gaatctatct tatgatatgg cattaagaca agcacaagaa ttaggttttg
     3121 ctgaagcaaa tccagagtca gatgtattag ggtatgatgc tgctagaaaa ctatcaatat
     3181 tatcaactct agcttatgac aatagagtat attggaaaga tgtgtattta gaaggaatta
     3241 cagatataga tgaaaaagat attgaatatg caaaaaaatt aaattgtaaa ataaaattga
     3301 ttggtcaaag taagtatgaa aatgataaag taagtgcttt tgttagacct gttttagtag
     3361 aaaaagataa tatccttgct agaatagaca atgagttcaa tgctgtcata gtgaatggag
     3421 attcagtagg agaagtttct tttgtaggta aaggagctgg aagtttagca acaggaagtg
     3481 cggtttattc agatgttata gatataatag ataatagagt ttcaagtata gattctttta
     3541 ctaaagataa aatacaagtt aataaaatag ttagagaaaa gtgtggagca cttcttagat
     3601 ttaaaaaatg taataaagat gaaattctaa atatagttga gaattgttta gtaaaattcg
     3661 atattttaaa tgatgatgat gaacttgcta ttatggttta tgctgattct gaatatgaaa
     3721 taaacaacag tttatgtcta ataaaagata aaggatactg cgaaaaaatg aataaaatgt
     3781 tgaaaataag ttaaaaaaga aaaaatataa gtttatatgt aacatatttt gacgatttta
     3841 atactatata gtatgagatg aaacaaaaca tctcataaaa aaattaaaat taaagggaga
     3901 gatatgttat atggaaaata aaaaatgtta ttcagaagat tggtatgaaa gaggagaatc
     3961 tacagctaaa tggttccaaa atgatagaga agaatatgaa agagaagcat atgatgaaga
     4021 tagagaaaga agaggttcaa actgtggatg ttcagattca ggagaaaata gacctagaaa
     4081 ctgtgaaaga tttagaagag aagctgagat aagagaaaga gaagcaagag aagcattctg
     4141 tgaatcttca gagaaaaaga aagaggcatt agcatatgaa tgtgaagcta gaaaattatg
     4201 ggaagaagca gaaaaatact gggatgaata ttcaaaatac aactataaag gaatcgaata
     4261 tttagcagaa gctgctagat tatttgatga aggtatggaa tgtgaagcta gaagaaatgg
     4321 aaataatgga ggaaacaata ataattgttg ccataaatgc cataaatgta attgtaactg
     4381 ctgtagaaaa taaaaaaatt aattgacaaa tcaatagtta aaatatatca tattatgtat
     4441 aataaaaaga aaattcagaa aaaaataatg attagaagaa atataagtat atttattaga
     4501 caaatagtgc tcagttagtg aggtgagtac aatatgatta aactcccatc ctctagcttt
     4561 tgatagaggg tgggagtttt tatttattga tatatcttaa atgaggataa tattgtaaaa
     4621 taaatgagta tgagattgta aaattaggag gtaaaaaatg aaaagtatta aaacatcaag
     4681 tatgaaagaa aaggtagatg aaaagatatt aagagagcaa gtaagttcta taatagaaga
     4741 tataagaaat aataaggatg ttgcacttaa aaaatataat gagaagtttg atagaaatac
     4801 aagagatgaa ttcagaatta ctaaggaaga aataaaggaa gcttataagc atgtagatga
     4861 cgaatttata aataacttaa agattgctgc caaaaatata agagaatttg ctaaagctca
     4921 aaaactaagt tttgaaaacc cttttgaaaa agaaatttat ccaggtgtaa tcttaggtca
     4981 aactaatatt ccaatagagt catgtcttgc atatgttcca ggaggtcagt atccattgtt
     5041 ttcaacagct cttatgctta taatacctgc aaaagtagca ggagtaaaaa gaatagtggc
     5101 atgttcacca actatgaaaa atactgaaaa gataaatcct aagacattgg tagctatgga
     5161 tatagcaggt gctgatgaaa tatatgcaac tggaggagtt caagctatag cagcatttac
     5221 atatggaaca gaaaagatta atccagttga cataattgta ggaccaggaa ataagtttgt
     5281 aactgaagct aaaagacaat gttatggaca agtagggatt gattttgtcg ctgggccaag
     5341 tgaagttctt ataatagcgg atgaaacttc aaatccagtt tatattgcag ctgatttatt
     5401 agcacaatgt gagcatgatt taaatgcaag aggaatatta ttgacaaata gcttggaagt
     5461 agcacaaaaa gttgaaaaaa atatagaaag tatgctaaaa gatttgccaa caaaggacat
     5521 agcatatagt tcatgggaaa ataatggaga gataatattg gttgatgata tggaagaagc
     5581 cattaaaata agtaattttt atgcaccaga acatttagaa gttgcagtaa atgagtataa
     5641 tgatatttgt gatagattaa ctaactatgg ttctttattt attggaaatc tttctgctga
     5701 ggtgtttggt gactatgttt caggtaccaa tcatacatta ccaacactta aagcatcaag
     5761 atatactggt ggtgtttggg ttggaacttt tatcaaaact tgtacaaagc agatatttaa
     5821 tgaaaaagct atacaatcat tagcacctgt agctgaaaag ctggcaaaag aagagggatt
     5881 atatgcacat gcaaaggctg ctgaggttag gtttaaaaag tagtaaaaaa ttttattttg
     5941 gcagtcgaga cttagtatta gactgagata aggaaaacgc tgagaacctg aataaatact
     6001 atattttgtg ggtctcggcg cttcttttat atctgtatat aatagaataa tactatctgt
     6061 gtgtttaaat actaacttaa tatttctttt ctaaataatg tttatgaaat agttaatatt
     6121 tttagttaat acttgaaaaa aacttttgct aactgtgcaa attcatctgc atttgtcaat
     6181 atagcagggt gtcctccctg actaaaaatg taaatacttg cttttgaaat aaaagttgcc
     6241 atttgcttgt actcttcttc aagattatga cgacacattt cgtcttcctt acttcctgta
     6301 aataaaattg gcatttctaa tttacacaat tctttatgaa acaaagggcg tttctcgttt
     6361 gcacattgca atagagcttt tgtatcaaga ttaacaacat tctcccaatc ttttccatga
     6421 caccattcat agaattttcg tgcttgaata tcttgctttg atttttccct tccagtgatt
     6481 agattatcta tgaagttatc attaagagta cgtccatcaa agctatctgc tataattgct
     6541 tcaactaaat cgggacgttc taatccagta ttaacagctg cccatgctcc tccacttgtt
     6601 ccaattaagc caactttacc acaattcaaa tgttcaatca gtgctattgt ctgtaaggct
     6661 tcatcatgcc atatgtcagg agaaaattga gaaactctat ccgactgtcc attacctaaa
     6721 aaatcaatta agatacatct aaaattttct gcatataatg gcacaagtgc tttaaacata
     6781 ttagaagaac cagtattacc atgcaggaaa attatcggtt taccttgtcc atattcttta
     6841 taaaaacaag aattaccttg atactgaaaa tatgacatat attttcctcc tttttatttg
     6901 ttaagttaat tataacaata taggtatgaa tgaaaaacct catgttcttt cttagaataa
     6961 aaatgtgcca cgcataaaac gtgacacatt tctaattata aattttgctg tttaagtatt
     7021 ctttgtatgc ttttctctgt caaaaagtat ttctctgata actgcttgat actcattcca
     7081 ttttggaaat ttatacagat ttgagaatta cgagaagcta agacttgttt ggtgtttgtg
     7141 ttttcacccc aatgcttttt gtgctcttgc tttttgggaa tataaatcac tttaccatct
     7201 atatattctt gcacttgctc aatcagttct ttaggcagta agtcaatcga attttgatag
     7261 ctcattttgc acctcctaaa taattattct ttaggattag caaaggctac tgccactcac
     7321 aattaaaaat aaaatttttt attatgtaca atagcctttg ctatgcacaa gttccaattc
     7381 ccataaacta aatactcctt tctggtttga aaaattcatc tgaacattga tttcgctttt
     7441 aagatagagt tcaatgacaa atttacattt ttattatata tattcagata gataattgca
     7501 actgtctttg tatgaggata ttttattata gtcaatacta taatatgtat ttatatcatc
     7561 aaactaatat gaacagttga aaggaatatg aacaataaaa cttagtcagg aagaataatt
     7621 atgaagcaga caatagagaa atttgatttt aaagttttat tgaaaattta ttgacaaatt
     7681 taacttgtaa atattatact aaacttaata aataaagttt ataaagtaag gtgttgtcta
     7741 tgaaaaatat agttggaaaa ccgcaaacga taaaaaaagt aaatgaagat ttaataaaaa
     7801 acattataaa aaatgaaggt cctataacaa aaccagaaat ttcaagcatt actgaattga
     7861 gtttagctac agttaataag attgtagagc aattagcttt gaaaaatgaa gttaaagtaa
     7921 gtggactaag tgaatctact ggaggaagaa gagctcaatt atttgaaata aatgcaaact
     7981 tagaacacat aatagcttta tattattata gaaattgttg tataggggta gttgccaatt
     8041 tattaggtga aataatttat caagaagaat ttaatataag aatggatacg tatgaaaacg
     8101 ttaaccaaga tacttttttt gttatagata ctctaataaa aaaggaaaaa ggaaacaata
     8161 taagggctat tggaattggt gtcccaggag ttgtaaaaaa aggtgtagtt aaaaatatac
     8221 caactataaa aagttgggaa ggtataaatt tagaaaattt aataaagagt aaatacaata
     8281 ctaaagtgtt tatagaaaat gatacaaata ttactactat tgggatttat caaaaacagt
     8341 ataaagacaa atataaaaat atggctctta tgtatttaga agatggtatt ggttcaggta
     8401 tagttataaa tggtgaattg tatttaggtt ctacaaattt tgctggagaa ttaagttatc
     8461 taaatataga atttggtaag tataactcta acaacttaaa tcttgaagat tatattatga
     8521 gtttaagaaa aagatattta gaaagtaaag ataataaatt taagaaagaa attttatcta
     8581 taatatcaaa ctttataagg actcttgtat gtgtcctaaa tccacaagct atgattatac
     8641 agtgtagtat attaaccaag agtgacatgg agtttataga aaaagatgta agcaataatg
     8701 ttggagaaga aaattttcct aaattaataa aagtagactc attgagagaa ggaagtataa
     8761 atggagtaat tagtatgtgt ttaaaagaaa ctgactatca atattcctta tctaataaaa
     8821 aaggaggata ttaatgtgaa atatttggtt tcaatagatg gagggggaac aaaaactaaa
     8881 ttttgtgtaa gtgacttaga tggaaacata cttaaagaac ataccactgg ttcaactaat
     8941 tataagtcag ttggaattaa aaaaacatat gaaaatatta ataatggatt taaaaaaata
     9001 ttaaaagatt tatatatcga ttatgacgat attgagtaca ctgtatttgg tatatctggt
     9061 tgtgattctc ctaatgatta taagattata atggatgaaa tattaaaaat tgggattaac
     9121 aaagaaaaaa tatatctggc aaatgatgca gttttagctt tttatgcaca agctgattcc
     9181 cctggtttag taattgtggc tgggacaggt tcaattatac tgggaattaa agaagatgga
     9241 gaaatatata gagttggtgg atgggggtat aattttagtg atttgggttc tggatatgat
     9301 atagggagaa aattattaaa gaaagtattg ctttattgtg atgaatgtca tgagtatagt
     9361 gatttattta gttgtgtctt ggacttcttt ggagcaaata gttttgaaca acttgcatat
     9421 atgattactg atataaatga taatgttgaa atagcaaact tagcttcatt agtgattgat
     9481 tgtgccgaac gaggtgataa attggctatt gaaatattaa gagaatcatc aactgaacta
     9541 tctaaattag ctcaagtaat attgagaaaa atatctaatc caatgaaaat ggataaaagc
     9601 gaaataaata tagttttgtc tggtggtaca ttaaataaaa ctgtatatgc tcaaatgtta
     9661 atgaataatt taaaaacaga aaatacagat aaaaatttaa aatttatatc tcaagaaaat
     9721 cagccagtat atggggggat aagacttgct atggcattag ctaataggag ggtcaatcat
     9781 ggatagaaaa aaggtaatag tacaaatttt tactggaggt tttaaggcta aagaaataaa
     9841 gtttaatgat ataaaatgta aactactaga attattggac tgtattgata tagaaaaggt
     9901 tatcatggga tggagtgtag atgaagaatt atatgtagaa actaaaaaac tattaaataa
     9961 atataatgtt gaactatatc tttggttgcc atgtttttct gaaataggat tacttgaaaa
    10021 aagtaattta ctaattgatt atatggggaa ggaagtcaag ggctatgctt tacaagagga
    10081 tgaaaatttt gagttttatt gcccaaataa taaggaaaat ataaaaaata taaagaaaat
    10141 atatagtact tattttagtg atattaaatt tgatggaatt tttttagata aaataaggta
    10201 tggttctttt tcgaataaac taaatggtgt ttttaattgt ttttgtaaag attgtttaaa
    10261 tatatatgaa aaaaataata ttgataaaga gtcgttaatg agagaaatga ataatgtgtc
    10321 taagggagtt atagataatg aagatactcc atttggtata acagaataca ttaatggaaa
    10381 atacaaattt aaaaataaaa tatgggaaga tttttttgaa acaaaaaatc aaaatatata
    10441 caaggcatta ttagagataa gtagttattt taaaagtaag ggattgaaaa taggtataga
    10501 tactttttct ccttttattt cttacttcac aggtcaagat ttagaaaaat tacaagaagt
    10561 agcagatttt ataaaaccaa tgatgtatag aactacaaaa gctccagctg gtttaccatt
    10621 tgaatttgaa aggtttattg atgaatccat acataagaat aaagagattg gaaaaagaaa
    10681 tttcttagat attttaggaa ttaaggagta tgaaggagat gtatacccta tagaatttgt
    10741 actagaggaa atcgatttta tgacaagaaa tcttaataaa gatgtatatg taggagttga
    10801 aataaataaa aaggaaatta tatctcctgt tacacctaag tacataagtg aaaatatttc
    10861 aaatataaat aagacaacag taaaaggata tgttttatct tgggatttgt tgtcagcacc
    10921 aaaagaaaat attgatgaaa ttataaaatg gtttcggaat tagtgaggtg ataaagtgag
    10981 taagatattg gaaatgcaac aaatatataa aagttttgga aaggtagatg ttttaaaagg
    11041 gataaatttt gagttagaaa agggagaagt aagagctctt ttgggagcta atggagcagg
    11101 aaaatctaca cttattaaaa ttttaggagg agttcattct caaactaaag gtaaagtaat
    11161 tttgactggt aatgaaataa tgtttaaaaa tccacatcat tctaaagaaa atggtattag
    11221 tataatttat caagaattaa gcttgatacc aacgcttaca gtacttgaaa atatttttct
    11281 tggtagagaa attttaaaag gaagtgtgtt tttaaacaag aaagacatgc ttgaggaata
    11341 tatgaaaatt tgtgaggatt ttgattttaa tatagaccca catatagtag tttctaattt
    11401 aagtgtagca aaccaacaaa tggttgagat aatgaaagct gtttcatgta attctgaaat
    11461 tatcataatg gatgaaccta caacatcact tactaataat gaaaaagatg ggctatttaa
    11521 aataataagt agactaaagt ctaaaggtaa aacaatagtc tatatttctc acatattaga
    11581 ggaagttttt aaagtatgtg atagtgcaag tataatgaaa aatggaatta tgattggaac
    11641 ttatgatgta gaggatttaa ctaaattaaa gataactcaa cttatgacag gaaatgataa
    11701 tcacaaactt attgagaaga aggattatac atttgcaaaa tatgaagagc caccagtact
    11761 agaagtaaag aatataagct ggtctaacaa aataaaaaat gtttcattta aagtttatag
    11821 aggcgaagtt gtaggtcttg caggtcttgt aggttcaaga agaactgaaa ttataaattt
    11881 gatatttggt atagataata aagatagtgg agatattttt atcaatggta aaaaagtaga
    11941 cataacttct cctaaggttg ctataaaaaa caaaatagga ttgattcctg aagatagaaa
    12001 aaatctagga ttaattttag aacaagctat atacacaaac tcatcatcag tacaaattga
    12061 taaattcaag aaattcttgt ttatagatag aaataaagaa attgagtttt ctaaaaaagg
    12121 tgttaaggaa ttaggaataa aagttagtga tgtaaagcaa gaagtaaaag agcttagtgg
    12181 tggaaatcaa caaaaagtag tagtttctaa gtggttagag aaagatttag atttaataat
    12241 atatgatgag ccgacaaagg gaatagatat tgctgcaaaa gaagatattt tcaatacaat
    12301 caaagaattt tcaagtaaag gaataggagt aatatttata tcttctgatt tagaagaagt
    12361 aataagagta agtgatagaa ttttagtaac tagagaaggt tctattgtag atgatttaaa
    12421 aaatgagaat atacaggttc aagatataat gaacagtata tttaatgtat aaaaaatgaa
    12481 aatggaggac tgaaatgaag aaagcttttg agaagttaaa tttaagagat tatggagtat
    12541 tattaggttt tttagcttta tgtatagcta taagtattgc aactccatca tttttaggta
    12601 aacaaaatat acttaatctt ttacgacaat cttctataat tggaataatt tcagcaggta
    12661 tgacttttgt tataatatct ggaaactttg atatatcagt tggagcagta gcagctcttg
    12721 caggtgctat aacaatgaaa tttgcgactg gtggaactaa tttgtttgca tccatgctct
    12781 taggtgtagc gatttgtgca gtaataggat taatcaatgg tatagtagtt gcaaagataa
    12841 atgtaccatc attaatagcc actatggcta tggtcactat tgtaagagga ttgctgttga
    12901 tgcttacagg aggatatcca attactgaaa atataccaat acttgattac attggaaatg
    12961 gatatttgtt tagtatacca ataccagtag taattttctt tatagtggtg ttagcttcgt
    13021 ttattgtact taataaaact aagtttggta gatatgttta ttctgtagga gggaatcaag
    13081 aagcaagtaa acttaatggt ataaatgtag attctcacaa agtgaaagtg tttattatta
    13141 atgctgtact tgcaggaata gctggaatcg tactaacagg aagacttggg actgcaactg
    13201 cgattgcagg tgaaggatat gatatggatg caattgcatc tgtagtaata ggaggtacat
    13261 cagtggcagg aggttctggt tctgtactta aaactgttat aggagtatta cttatgagtg
    13321 ttataaacaa cagttttaat cttcttggaa tagatgtata tttccagtac atatttaaag
    13381 gattaataat tttagcagca gttggatttg attcttatag caagaaaaaa cttgcttcaa
    13441 gataaaaaat aagggggatt tttttatgaa aaaattaatg aaacatttag cattgttgtt
    13501 atcacttgtt atgatatttg gtttagttgg ttgttcaaat ggtggtgaca aagaaaaaaa
    13561 tggagaaaag aaaatagcag tattactacc aggttcaaca ggttactttg tagcaactaa
    13621 acaaggtata gatgcaaaat ctaaagaatt aggtgtaagc gttgaatatg cagatgctca
    13681 atgggatgct agtaaacaat tatcacaagc tgaagatttt atggctaaag gggtagacat
    13741 gattgcatta tgtggagttg attctgctgt aagtgagaga atagttaaag ctgctaatga
    13801 ttcagacgtt cctatagtag catttacaaa ttctatagga agtaacccaa ctggagaatt
    13861 taaaggttta gttacatata tagggcaaaa tgaggaagaa acaggagcat taactggaaa
    13921 aattgctaaa aacttattag gagaaactgg tgggaaagct gtattaatag aaggagtacc
    13981 aggaactact ccacaagtat taagaaaaaa aggtcttgaa aaagaattaa aagatagtaa
    14041 tatagaaata gtttataatc aaacttcaaa ttgggagaaa gagcaagctt taaaggtaac
    14101 agaagattta attcaaaaga aaactgattt taatgttata atatgtcaag atgataattc
    14161 tgctacaggt gcaggtcaag ctttaaaaga tgctggtctt aaggataaag ttaaagtcat
    14221 aggtttgggt ggaagtaaag atggattaaa agctataact gatgggttaa tagatggtac
    14281 aacttatatg tctgctgtag aagaaggtgg attagtcata gaaaaggcat ctaaattcct
    14341 taaaggtgaa aaaatagaac ctgttacaca aattaaacaa gttgaagtta acaaagataa
    14401 tatttcagag ttcaagggtg aatggtaaat tgtcatagat aataaataag atttttagag
    14461 ttactttaga aactatataa agtaactctt tttttaattt aatttagtga taaattattg
    14521 aatagtattt ctaaacaaat aaaaaattaa tataaacaaa gacttaatta tactaaatta
    14581 taagtaaaac taatataaac ttatggaaaa aaggaaaacg tctaaagcat tggaattact
    14641 atagtgtagg tgtggtatta aaaaaacata aaaaaacata aaaaaattgt tgaaaaaaat
    14701 atagaaattt tgtataatta aagatttgaa aagtgcggta tacaaagcca attcttttgt
    14761 atataatata tataaattca gaaaaaggaa gggatatgta tggccaggcg gttattttta
    14821 gaaaggattg gacttatatt tttaggttca gcaatattag catttggagt ttataatttt
    14881 tattatttaa acaacataac agaaggtgga gtgttaggta ttttactact gttaaaaaat
    14941 ttattcaata tacaaccagc aatagcaaat gttgtaatag atggtttact acttttagta
    15001 ggatataaat ttttcggtaa aaaattcctt atatattcaa tagtagcttc tataactttt
    15061 tcagtgttat atgacttatt tgaagctata ggacctctag taccacaatc acaaaatatg
    15121 cttttatcaa ctattttggc aggtgtaact gttggaactg gtgtaggtat agtagtaaaa
    15181 gcaggttgtg catcaggagg agacgatgct ttagcattag taatttctaa gactacatct
    15241 ttgaacatag ggcaagttta tttagcaaca gatgttattg tattgttgtt atcattattt
    15301 tatctatcag cgtttgatat cttttattct ttgattgctg ttacaattag tggcaaggta
    15361 attgatttta tttattatca tggtaaaagt ttggatatgg atataagtaa tgatatagtt
    15421 ccagaatgtt aatataaatt aaataacttt agattttatt aggggtggca gatttgccac
    15481 cttttttgtg tctaaatctt tatagaaagt ataatcccat ctaggttttt gcaaatctca
    15541 aatatagaat tatgttggaa tgactgggaa tacgattgcc agatgtgcat tttaaaaacc
    15601 aaattttata tttatgaaat actaatcatt aatggatgtt tcttaatcct atctttatac
    15661 ctaaaatgat aagataaatg aagtagcaat acaaagctaa aatttgtaaa agagattaaa
    15721 ttaaatggag gagctaaaat gttggatata gttatgatag cattgttatt cataggattt
    15781 gtttccatga agttacttgc tgattggtgt gggaaacaaa ctgaaaaaaa ataatgtaat
    15841 gtatggaggg attacctatg tggtttctgg cagttgtaat tataatgtta ataatatatt
    15901 tggtatatgc gcttattaat cctgaaaaat tttaaacagt ataaggctaa taagtaagtt
    15961 gaattggggg gaacaatttt tatggtgcag atagtaatag tgttagcgat ttttatgatt
    16021 ttagtaatac ctatgggcaa gtatttatat catatagcta ctaatcaaaa aacatttgga
    16081 gatagactat ttgataaagt ggacaatttt atatataagg tatgtagtat agataagaaa
    16141 aaagaaatga actggaaaca gtatgcatta gctttattat ttacaaatgc agttatggta
    16201 tttataggat atatcatact tagaactcaa agtatgcata tttttaatcc aagtggaatt
    16261 aaaagtatgg aacaaggact atcatttaat acaataataa gttttatgac aaatacaaat
    16321 cttcaacatt attcaggtga atcaggactt tcatacttta gtcagatgac ggtgataata
    16381 tatatgatgt ttacatcagc tgcaacagga tatgcggctg ctatggcatt tgtaagaggt
    16441 ctagtcggta aaaagaaaac attgggaaat ttttatgtag atttaattag gataacaaca
    16501 agagtacttt taccaggagc tttaattatt gggcttatat tagttacaca aggagtacca
    16561 caaacttttg ctgggacaga aacagtgact actatagaag gaaagcttca agatatagct
    16621 agaggtcctg tggcagctct tgaatcaata aaacatttag gaacaaatgg tggtggattc
    16681 tttggttcaa attcatcaca cccatttgaa aatccaacaa taatatcaaa tatagtagaa
    16741 atattatcta tgatgatatt accaggagct tgtgttgttg catttggtca catgataaag
    16801 aataaaaaac aaggttgggt tgtgtttggc gcaatgtcaa tcatattttt aataggtcta
    16861 gtagtgtgct ttaaagcaga aagtgcagga aaccctattt tatcacaatt agggcttaat
    16921 caaagtatgg gaagtatgga aggtaaggaa gttagatttg gtatagcaca atcatcatta
    16981 ttcacaacag tgactacttc ctttacgaca ggtacagtta ataatatgca tgatacactg
    17041 actcctttag gaggattagt accactttta aatatgatgc taaatgtagt atttggtggt
    17101 aaaggcgtag gtctgatgaa tatgttgatg tacgctataa tagcagtatt cttatgtgga
    17161 ttaatggtag gaagaacacc agagttttta actaagaaaa tcgaaggaaa agaaatgaag
    17221 cttattgctt tacttattat attacatcca cttttaatac taatgttctc tggtctttca
    17281 gtagcaatac cagcggggct tgaaggaatt tcaaatcctg gattccatgg attatcacag
    17341 gtattgtatg agtttgcatc ttcagcagct aataatggtt caggatttga aggtctagga
    17401 gataacacta tgttttggaa tataacaaca ggtatagtta tgttctttgg aagatatgtg
    17461 tctattatag tactactagc aatatcaagt ttgttagcat ctaaaaaagc tgtaaatgaa
    17521 agtataggaa cacttagaac ggataatttt acatttacaa tagttttagt acttgttgtt
    17581 ttaattgttg gtgctttaac atttttccca gccttagcgc ttggacctat ttcggagcat
    17641 ctagtgttgt ggcactaaaa aattgggagg gagtaaagtt ttatggaaac taaaaaaaca
    17701 aagtttatta caaaagatat tttcaaaact tccattatag aagcttttaa aaaattaaat
    17761 cctaaatata tgatgaaaaa tccagttatg tttgttgtag aaattggatt tatagtaaca
    17821 ttgatactta caatagtacc tagtttgttt ggtgatgaag gcaataactt aagagcttat
    17881 aatgggattg taacaataat cttatttata acagtccttt ttgcaaattt tgctgaagct
    17941 gtagctgaag gaagaggtaa agctcaagca gatacactta agaaaactag aaaagataca
    18001 gtagcaaggg ttatagatac gttaggaaat gaaaaaatga taagtgcaag tgagctaaaa
    18061 cttggtgata ttgttttagt aaatgctgga gaagttatcc caaatgatgg agaagttata
    18121 gagggtatag cttctgtgga tgagtcagca ataactggag aatctgcacc tgtcatgaga
    18181 gaatcaggtg gagatttcgc atctgttaca ggtggtacta cagttgtaag tgactggtta
    18241 aagataagaa taacaacaaa accaggagaa tcgttcttag ataaaatgat agctcttgta
    18301 gaaggtgctt caagacaaaa aacgccaaat gagattgctt taaatacttt gttagtaggg
    18361 ttaacactta tatttatggt tgttttaata tctctttatc caatggcaaa ctatgcaggt
    18421 gtaaagattc caatttctac tatgatagct cttttggtat gtttaatacc aactactata
    18481 ggtggattgt tgtcagctat tggtattgca ggtatggata gagtaactag atttaatgta
    18541 attgcaatgt caggaaaagc agtagaagct tgtggagacg ttgatactat gattttggat
    18601 aagacaggta cgattactta tgggaataga cttgcatcag aatttatagc agttggaaat
    18661 gcagattcaa aagatttaat aaattattct gttatgtgtt cattaaaaga tgatacacca
    18721 gaaggaaaat ctatagttga tttaggtatg aaattaggtt caacaggtaa gacaaaagag
    18781 gcagaggaag ctgagtttgt agaattttct gcacaaacta gaatgagtgg tattaactta
    18841 gctgatggta gagctataag aaaaggtgct tatgatagca taataaaaag agtaaaagaa
    18901 tctggtggta cagtgccaga agatttagaa gaaaatgtaa atcgagtagc taaactagga
    18961 ggaacacctt tagtagtgtg tgtagataat gagatatatg gaattatcta cttaaaagat
    19021 acagtaaaat cagggcttgt agaaagattt gctagacttc gagagatagg aataaagaca
    19081 ataatgtgta caggggataa tcccttgaca gcagctacaa ttgcaagaga agctggggta
    19141 gatggattta ttgcagaatg taaaccagaa gataaaatag atgcaataaa aaaagaacaa
    19201 ttagagggta aaatagttgc aatgacaggt gatggaacaa atgatgctcc tgcacttgct
    19261 caggcagatg taggtcttgc aatgaatagt ggtacaacat ctgcaaaaga agcagcaaat
    19321 atggtggatt tagattcaga cccaactaag atattggaag ttgttgaaat aggaaaacag
    19381 cttttaataa ctcgtggggc tttgacaaca tttagtatag caaatgatgt tgcaaaatat
    19441 tttgcgatta taccggctat atttactctt gcaatacctc aaatggagat attaaatgta
    19501 atgaaacttt ctacgtcatt tagtgcgata ttatcagctt taatatttaa tgctataatt
    19561 atcccatgtt taatacctct tgcaatgaag ggagtaaaat atagacctat gaaatcacaa
    19621 gctatgttaa tgagaaatat gcttatattt ggtcttggtg gagttatagt accattttta
    19681 ggaataaaat taattgattt attgattaca cctttagtaa tggttcttaa tttaggttaa
    19741 taaatggagg atataaaaat gaaaacatta aagccagcat tacttgtaag tattgtattg
    19801 ttagttgttt gtggacttgt atatccactt gttttgacag gtgtaagcca agttgcattt
    19861 aaagataaag ctaatggaag tatgatagaa gtaaatggtg taaaagtagg ttcagaactt
    19921 attggtcaaa gctttacaga tgcgagattc tttaagggaa gagtatcatc agtaaactat
    19981 aatacttata caaaagaaga tttagtacca gataaagatg gaaatacatc ttatggaggt
    20041 gtatcatcag ggtcttttaa ttatggagct acaaatccag aacttcatga tagagtgcaa
    20101 aaagatatag aaaagttttt aaaggataat ccaactgtca aaagagagga tataccaaca
    20161 gatttactta cagcatcagg ttcaggactt gaccctaata taagtccagc ctctgcaaaa
    20221 attcagatac cagctatagc aaaagcgtct ggaatatcag aaagtaaatt acaaaaaatt
    20281 gtagatgata atacttccaa aaaacttttt ggagttttag gtgaagatag agttaatgta
    20341 ctaaaggtta atgtagaggt cgcaaaaata ttaggtctta tatagtattt tgtagaattt
    20401 tagtaacaaa atattacgca agatttaatt taaaggttaa attttaaaga gtgaaaatca
    20461 ataatgattt tcactttttt atttgaatat tactaatatt tggaataaag tgattgtaag
    20521 tataaaatcc aaaaaaaggt tgcattatca aaatgataat actataatat atttatcgtt
    20581 atcaatttga taatagattt ggacatatag gaggaaatag tatgttatat aataacgcat
    20641 tagagttgat aggaaacaca cctgtagtaa ggctgaataa tttaggctgt actaatgggt
    20701 gttctaacat atatgtaaaa ttagaaaaag taaatccagc aggaagtata aaagatagag
    20761 ctgtttatga aatgttagag ggcttagagc aaagaggaga attaaaaaag ggagatgtac
    20821 tagttgaagc aactagtgga aatactggta ttgcattatc tatgatagga aaattaaaag
    20881 gatatgaagt agtaatcgta atgcccgaaa caatgagcgt tgagagaaga gatttaatga
    20941 aggcttatgg agcaaatttg gtgcttacag atggtaaagc tggaatggca ggttccatag
    21001 aaaaagcaaa tgaactttta aaagaaaatc caaactataa aagcctaaaa caatttgaaa
    21061 ataaagataa tccaaatgca cattataaaa caacagcagt tgaaattatg aatgatgtca
    21121 aagatttaga tatattcgta tgtggagttg gaacaggtgg tactttaata ggaactgcaa
    21181 gatatttaaa agaacaaaat ccaaatataa gagtaattgc acttgaacca aaaaaatctc
    21241 cagctatatc agaaaacaag actggacctc ataaaataca aggaataggt gctaactttg
    21301 ttcctgaaaa ttatgattct agtgtagttg atgaagtaat attagtagat gatgaagatg
    21361 catttgatac agttaaatta cttgcagcaa aagagggaat tttagtaggg atatcttctg
    21421 gagcaaatat atttggtgct atagagatgg caaaaaagta tccagataaa aagatagtta
    21481 cagtagctcc agatggagtt gataagtata tgtctatggg aatattttaa taattttatt
    21541 tgagggagtg aatactttgt ttaagaaaat aaataaagat attgaatata taatgaagaa
    21601 tgatccagca gctaggtcta agatagaagt ttttttactt tatccatcag ttcatgcaat
    21661 gataatgcat aggatggctc atgctcttta taagaagaag aagcttttta cagcaagatt
    21721 aatatcccag atatccagat ttatgactgg catagaaata catcctggtg ctaaaatggg
    21781 ggaaggtata ttgatagacc atggaatggg tgttgtaatt ggagagacag cggaagtagg
    21841 taatagggtg acaatatatc aaggagctac tttaggagct acaggaaaag atactggaaa
    21901 gagacatcca actgttggtg atgatgtatt aattggtgct ggaacaaaaa tactaggacc
    21961 tttaaatata ggttcaaatt caaaaattgg tgcaaattct gttgtcgtta aggatgtacc
    22021 taatggtgca acagttgttg gtataccagc aaaaatagta aaaataagaa acctagaacc
    22081 tgtaaaaaag aataaaaaag aagtgtccta tgagtatgat gaattagata atgtatatta
    22141 tatataggat ttatataact ataaaataaa ctttataaaa attattttaa aaggtatttt
    22201 tttcaaattt gaggacataa taaaaaagaa ctgaatgtta cagttttggt tcatcaatct
    22261 ttgataaaca agattttaaa tttgtagcgt ctaggaggat tttattatga aagcaaatgt
    22321 taatcaagat acttgtatag gttgtggatt atgtccatca atatgtccag aagtatttga
    22381 tatgaaggat gatgggaaat cacatgttat agttgatgaa gtaccaagtg atgctgaaga
    22441 atcagcagca gaagctagag aaagctgtcc agttgatgca atagatgtgc attaataaaa
    22501 taaatgccta taaaaattga attttcaatt tttataggca tttgtctttt attcagttag
    22561 tatttcaaca cctgtttctg taactaaaac catacattcc cattgagcag ataatttacc
    22621 atcttttgta taaatagtcc aattattttc ttcatcaaca aaaatttcgt gagttccttc
    22681 atttaccata ggttctattg tgaatatcat cccaggaacc aatagcatct cagtcccttt
    22741 tttggataca taacttacaa aagggtcttc atgaaagctt aatccaactc catgaccacc
    22801 tatttctcta acaacagagt atccattttt ctttgcataa gagttgatag catcacctat
    22861 atctcctaaa aatccccatg gtttggcaac ttcaagacct ttttggacac attcctttgt
    22921 aacttgaact aatttttttg cattttcatt aacattacct atcataaaca ttcttgaagc
    22981 atctgaatag tatccattaa aaatagtaga aacatctaca tttataatat ctccatcttt
    23041 taagataatg tccttacttg gaataccatg acaaacttca ttatttactg atgtacatac
    23101 acttttagga aatccattat agtctagtgg agcagggata gctccatttt gtagtgtaaa
    23161 gtcatgaacc aacttattta tatcttctgt agacattcca atttcgattt tatctgaaat
    23221 ataatccaaa atttttgtgt ttattacagc actttttttt atttcctcaa tttgactttc
    23281 attttttata atatcatgag ttggtacaat atgacctaac ttagcaaatg aatctatttt
    23341 attatccata tccatatgac attttttata ttttttagta cttccacacc aacagctatc
    23401 atttctattt aacttcataa cttttcatcc ttcttttatt atttatttaa attatgggtt
    23461 acataaacta ttttaaaaca taaccattat aaatattata acataaatac taagaaatcc
    23521 tttatagata gtgatttatt caaagtataa attattatat ggttattgtt gatgaaaaga
    23581 ggatagttaa atgtattatt ggatattagc gcacttaata gtttagttca agtttttcaa
    23641 gaaaatcata tgttagagga caattttcct ttaaagtatt ttttgaatct tcatcaagat
    23701 aataatgtac aaatgattct gcaaaaaatt cttctataaa cttgcttata tatgcatgtg
    23761 caacttgctt agggtcataa agcttatatc tttcctgagc aaatacttcc ataaattctt
    23821 cagaatttga ttttttattt aaaacatttt tatctatagc atgtgcagtt tcatgtaatt
    23881 ctaaattttt tgatgaatgt acatttgcat agctaggatt actataacct attttagcca
    23941 ctatagaatt atttctatat ataccaggta catctttcca agtcttgcct gatcttaccc
    24001 aacatggagg taattgatat tttaaatcac taaattctgg ttcatcagtt agatttgaat
    24061 ttattaattt tatttttata tccttttctt taagtgtctg taaatattta ctatttattt
    24121 ttgcaagtct gtttttcata ttttcaactt catctttatc ataattcaag tcatcaggaa
    24181 gtataagcat ttgttcaatt atatctatac tactgtcttt tatattttca gattcttcat
    24241 caaatttata ttgaagagta ttattttcat agatagttat atttgtatta ttttctatag
    24301 aataagagtg aatatcctct aaatctaaaa tattcatttg ttgtaaaata tgttttgttt
    24361 cgccactcca tactactaaa gaaagtgaat taaaattaat tagtggattt tcttttgctt
    24421 ttatatcatt gcttatatct acatcaaata catattcaat tgagtcttct atgttaagac
    24481 tatctgtatt ctttttaata tataaatacg cttgttttaa gtctatataa atctcatcta
    24541 aagattcgtc ataaaattct gataaattat tagtaaagag ttcttcactt aaaagttcat
    24601 ttttatcagt tatactttca attaaaatat taatttcttt tatcatatcg taaatactta
    24661 tttttctatt tgctttttca ctagatacat tgcatggcgc aagagcaatg caataaaaat
    24721 ctttttcttt tttataaaag gctttaagtg attttgataa aagtgtaaag tctacaccta
    24781 tctgtaagtt gaccttaagt gagtcatttt gtaataattc agtattattt cttttagctg
    24841 tatttaaatt gttttccata atcattgccc cctttttatt agggtatcat aattttttta
    24901 tatgtgtcaa tttgtttttt tgaaaattat aatttttttg aaaattttat tttttataat
    24961 ttgtgaattt tttattatag aaataaatat gttattatta tgtcaaaatt tagcattaga
    25021 ttcatgtaga gtatgtttta tacatatatt ataattaatt ctttgaagat tgcatattca
    25081 aaatacacac catttttttt aattctataa tataatattt atatatttaa tgttaggatg
    25141 tgatttaagg tgtattttac atatattata agatgtaaag atgattctct atacactgga
    25201 tataccagta atatagttag aagaatgaat gaacataaat taggaattaa ttcaaaatac
    25261 acaagagcaa agggatttga aaaactagaa gtttattttg tgaccaacac aaaaagcaat
    25321 gctatgaagt tagaatatta tataaaaaaa ttaactagaa ataaaaaatt gtctataata
    25381 aaaaacccaa gtatacttat aaatttaatt gataataaag aagattatat tattggtaag
    25441 gaaatagaac aattaacata aatatttata gtaacattat aatgggtgca tatggtgtaa
    25501 aaacttgaca ataaatacta ttgtaatata atgaagttat aaatatagta aattaaaata
    25561 gctaggggag ccaagagctg agaggtatac cgacccttac acctgatctg gataatacca
    25621 gcgtaggaaa gcttagtaaa aattcaagta tgtattattt gtgatatatg ctatttctaa
    25681 gctataccta ttgtaggtat agctttttta attaagttat ttaaattaaa ctttaggagg
    25741 caacaatgag taattataaa ataccaacac ttacaatagc tggatctgat tcatctggag
    25801 gagctgggat tcaggctgat ttgaaaacgt ttagtgcgat tggtacttat ggtatgagtg
    25861 taataacagc tataacagca cagaatacta aaggagtttt tgctgtagag gatttaaata
    25921 agaaaataat aaaaaaacaa atagaggcag tctttgaaga tatacctcca agagctgtaa
    25981 aaataggaat ggtttcaagt cctgagatta tacttgaaat agttgagaat ctaaagaaat
    26041 acaatccaaa atatttggtt gtggacccag taatgatatc taaaagtgga tattatctat
    26101 taaagccaga ggcaaaagaa aacttaatta agtatttgat accacttgca tacataatta
    26161 caccaaatat accagaggca gaagaaataa caggaataaa aatacataat gtagatgata
    26221 tgaagagagt aggagaagaa atactacaac ttggacctaa atttgtgctt atgaaaggag
    26281 gtcatttaga tggagaagca gtggatattt tagttggtaa aaatatattt aaagtttata
    26341 aaagtgaaag aattgataaa aaaaatacac atggaacagg gtgcacatta tcttctgcaa
    26401 taacatctta tctagcatta ggatatgaaa taacagaagc agtaaatcta agtaaaatct
    26461 atataacaga agcaataaaa agaagttttg atataggtca tggagtagga ccagtacatc
    26521 atttttataa atttgagtaa taaaaaaata ttggaggtag aatatgtata atttaattaa
    26581 agatgtaaaa aaattaaatc cattggtaat tcattatact aataatgtaa ctataaatga
    26641 ttgtgctaat gtaactttag ctgtaggagc aagtcctctt atgagttttt catatgagga
    26701 agtagaagaa atggtaagtg tagcaaactc tgtagtaata aatataggaa ctatgaactc
    26761 aaatatgttg gatttattcc tattagctgg aaaagcagca aataagtaca ataaacctgt
    26821 tgttttggac ccagttggag tatttgcaag caaagcgaga gcagaactta caagtagact
    26881 tttaaatgaa gtcaaattta gtgttgttaa agggaatgta tcagaaataa agtttatagg
    26941 tggctttaat gtaaggggta aaggtgtaga ttcatttgat gaagaagaag attctactga
    27001 gataataaga aagattgctg aaaagcttga atgtgtagtt gtagcaactg gaaaaataga
    27061 tataataact aatggtaaag gtacatataa aataaacaat ggaactgata aattaaaagg
    27121 aattacagga actggatgta tgactgctag cttgattgca agttttatgg ctgttactga
    27181 gaatatatta gaagcagcaa caatgggtgt tcttacaatg tctttaagtg gggaattagc
    27241 taatttaaat aatccaccaa taggtacatt taaagaaaat cttatgaatg ctatttatca
    27301 aatggatata gatactttaa gtaaaaattc taatattgaa tttttaaatt aataaagagt
    27361 aagggagact taaaatgatt gataaagaat ctttaaaaaa atgtttaaag ttgtatttag
    27421 taactgactc tgaaatgctt aaaggtagag acttttataa atgtcttgaa gatgcaatat
    27481 cttctggaat aacaactgta caacttagag aaaaaaatgc ttctggaaga gagtttttaa
    27541 gaaaagctat gaaacttaga gaaataacta aaagatatgg tgttaagttt ataataaatg
    27601 atagagtgga tatagcactt atttgtgatg cagatggagt acatgtagga cagagtgaca
    27661 ttgatgttag agaggttaga aagcttatag gaaacaataa aattctagga gtatcagcaa
    27721 gaactttaga agaagctatt tgtgcaaaaa atgatggcgc agactattta ggtgtagggt
    27781 ctatattcac aacatctact aaattagatg caaaatcagt ttcgtttgaa actgttaaag
    27841 aaattaaaga aaaagtagac atgccatttg tactaattgg aggtataaat ttagacaata
    27901 tagataaact taaatgttta gaaagtgacg ggtatgctat aatatcagct atattaaaag
    27961 ctgaagatat ctctaaagaa gtggaaaagt ggacattaaa aatataaaat tggctctggt
    28021 agtgaaaaaa atggaaaaat ttgatataat ctaagagtaa aatttaagca attataaatg
    28081 ataaactttt ctacatatac tttttaatca agatatatgt agatttggtt aaaaaggagc
    28141 tataaatgat taggatattg ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa
    28201 ataaaatgga gaaggatgca aaaaaaagaa atatagactg taaaatatgg gcagttggag
    28261 aaggagatat aaaatcggag cttgataact ttgatatttt acttcttgga ccacaactta
    28321 gatttatgct tgatgacgta aagtctatag ttggagatag agctccagta tctataatag
    28381 atatggtaaa ctatggcact tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat
    28441 taaaataaat aataaaagtg gattctatat aatagagtcc atttttatta tttgttatta
    28501 ataggcttaa aacttagctt accatttctt tttttaatct ccacatagca agtgaaaaac
    28561 ttgaacagca caatattaaa aatagtagga taacaggcag tatatctcca ataagaatag
    28621 tctcaccaaa tattaagcta cgtaaagcgt taataacatg ggtaaatgga ttgagcatca
    28681 ctgctacttt taatcctcca gataaacttt ctatagggaa aagagctgaa cttaaaaaga
    28741 aaataggaag tacaattgca gtcataattg tttcataaac tatttcattt ggcaataata
    28801 aacttataga ataagctaga cttgataaaa agaaggctgt cataaatatc agtactatca
    28861 taagtaggat tccaccaatg ccagattcta tccttactga gaagaatata cttactatac
    28921 acaaaatagt tacttctatg aaagaaacta agatagcttc taataattga ccaaggacga
    28981 ttgaatacct actgataggt gcaattaaga ctctatagaa acttccactg ttcttcatta
    29041 taaagtttat aataccacca ctactacaag aagaaaacac aaccagtaca atgatacctg
    29101 gtagtataaa agcagtataa ttattgatat ttatattatt catagtttgg tttgcaatag
    29161 aactataaag cacaagccaa agaaaaggtt gtaatatagt aactacaaat gagattggat
    29221 ttttaaaacg ccacttcata ctacgccata agatatttaa cattcccatt cctcctttaa
    29281 gcttttttta gaacttgtaa gtgctaaaaa tacatcttcg agacttggct ctacaatttc
    29341 tattgcatca aattctatat catgttctaa aagccattta tttattaaag taaaagcagt
    29401 tctactatcg tttacactta taaaaattga attttctcgt acgctcataa actttactaa
    29461 accagtactt ttaattgcat ctttatattt ttttatatct tcagtattat ggaaagttat
    29521 acgtagtata ttttgtctaa tgtaactacg taaacttgat ggtgttcctt gtgctaaatc
    29581 ctttccattc ttcataatac aaatattatc actcaattgt tcagcttctt caagataatg
    29641 agtagttaga aatatagttg tgccatactc atctctaatt tttaataaca tatcccacat
    29701 agattttctt gaatcgacat ccataccaac agttggttca tctaaaaata ggattttagg
    29761 agatgatacc atattcatag ctatgtccaa tctacgtttt acaccccctg aatatgatga
    29821 agtaggatat tttaagtaac tagataagtc aaacttatca attaaagagt ctattctttg
    29881 ttttgcaatc tgtggttcta ctttatacat tttactttga aataccatat tttccataag
    29941 ggataaatgt tcatcaatag agatttgctg tgctacacaa gcaatttgag tacgtatcca
    30001 agaaggatta tctactaaat cttttccaaa cattgttaca tttccagatg taggtttgta
    30061 aaaggtagtc aaaatattta taagagaaga tttaccagca ccattaggac caagtaaaga
    30121 aaaaatttct ccagcattta cattaaagct tagagcattt aaagctttca caccattttt
    30181 atattctttt actaaattgt ctatttcaat cgctaacata tattattcct cctctttaag
    30241 tggaaatatt atctctgtaa tatagttatc tgggtttcct tttatcaaca tacctggtcc
    30301 tttaataaat acttctctcc aaggagtttg tatttttata ttattttcat gaatatattt
    30361 aaatatcatt tcatacgttt ttggaaaatt actataagag cctgtgtgag taaaaaataa
    30421 agcttttgtt ttaggaaatt cttttgtagt tatacccata cgatttggag tttcagcagt
    30481 tggaacacat aattccattt ctgcaacacc agttttttgg tcaacatcat agtagcaaaa
    30541 gaatggtgca ccgttagact ttccttttat agctttaaat acatttggaa aatactttgt
    30601 tgcctcagtc ataggtccat tataacgcat ggtagccact gtgactgatt cgatattttt
    30661 gatttcaatt ttgcaattca ttgtttacct cctaatgtgt gttcatattc ttactagatt
    30721 tttaagcatt aatacatata agattttgat tagttgaaaa catataatct tatctcgttt
    30781 gcttgttttc aagtctaaca taacataaaa tatatatcct ctcatttgat gctccatttt
    30841 gtaaagttat tatttaagct ttactttaga ttttttagca aattaaaata aagcaattta
    30901 taaaataagt atttacaaat taagtgtatt aatcgtataa tacacttaat aggatactag
    30961 ataaagtctg aaataaaggc gtattttttt aaagtaagtg tactatcgta ttaatacact
    31021 agaataggag gaatcatgaa ttttgaatta gataatacaa ctcccattta tttgcaaata
    31081 gtaaagtata taaagagaca aatagtaaca ggagagttga aaccaggaga gacaatacca
    31141 tcaagaagag aaatggcact taatctaaaa gtaaacttaa atacagttca aagagcatat
    31201 aaggagatgg gtgatatgaa tataataaat acttttaaaa attatcaaag cagtgtaaca
    31261 gttgatgaaa atatattaaa aaatttaaaa ttagagctta taaatgaatc actatctgtt
    31321 tttatagaag atatgaaagc aataaatgtt agcaaagaag aagttttaaa aataatagaa
    31381 gataagtatt agttttttaa caaatagaaa acttagttta gaaagtttat ttataagcat
    31441 attggatgtg gaggatagta aatatgatag aaataaagaa tgtcagcaaa acatataaaa
    31501 ggatgcaagg gcttaaaata agaaagatag aagctttaaa aaatgtaagt tttaatatag
    31561 aaaagggaaa aataactgca ttacttggta taaatggtgt tggtaaatca actatgttaa
    31621 aagcaatagc aggacttata aacattgata gtggagaaat tcgtatagat ggagaaaaaa
    31681 taaatgaaaa ggtatacaat aaattggctt ttgttccaga tgtacaatct cactttagca
    31741 atacaacaat aaaagaaaca tttgaattta tggaaatttt ttattcaaag tggaataaag
    31801 aaaaatcaaa agaaatgatg gacatattta aattggatga agatgaaata attgataact
    31861 tgtcaaaagg aaacattgca agagtaaaat taatacttgg attttgccaa gacccagagt
    31921 acatacttct tgatgaacca tttacaggaa ttgatttgtt taagagggaa gagtttatag
    31981 gagtaatagc gcaatatatg gaggaaaatc aagccattat cataactact catgagatag
    32041 tagagataga aagtttagtt gatgaggttg taattcttga tgaagggcag attataactt
    32101 catttaatgc agaagaatta agagaaagag aaggtaaatc tatattagat aagatgaggg
    32161 aggtatataa gaatgaataa acttctatat ctaataaatg tagatttgag aagaagcaat
    32221 aagttttata tagcttatat atcatttttt tcactaatta cacttagttt aaatttattc
    32281 gaaattaata aatttaaaaa ttctaaattt attatgaact tagcaataga agattttgga
    32341 ggtatatttt atggactagg gatacttagt aatcttggtt ttatccaaag tatattgttc
    32401 tttgggatat tgggactttt tatatactat atttttatgt ggaaaagaga gttcttttca
    32461 aataatcaaa gtatttatac acttatgatg ttacctcaaa ataagtttaa gatttgtata
    32521 tctaaatcaa tagctttgat aacaatgata tatggttttt tgataagtca gacagcaaca
    32581 ttatttatat gtaaatatat atttaatttc atttttagaa atatgcctat aatcaatatg
    32641 aactttgcca aagatttata ttatttaaac ttgaagagta taccaattga ttttatctct
    32701 tttatggcta tctatgtatt tttactactt atagctattt ctattgtaaa ttgctgtata
    32761 ttatttatct tttcctatat aaataaattt tatgtaattt taatcacttt gctgattata
    32821 gcaggcttat atttttcaac aatatatata aatataatca ctttatttag aaaatattat
    32881 agtcttatag gatttcaatt taacaattta gtagcagggt tattaagttt tttaattata
    32941 atgtttattc taaatggaat atcttatttg ctaattaaaa agaaagtatc tctttaagga
    33001 ggggtggtat gaaattaaaa tctaaaatat ctatacttat tatatttttg actttagcaa
    33061 tatattttag tataggttta ttaaataatg aagataattt aaagataaaa actataaaag
    33121 gtgactctaa tgaattagac catataaaca taaattttgg atatgctttg aatggatata
    33181 taaattcata ttatacaata aatggaagta acaaaaccaa aaagactgaa ttaataaaac
    33241 tacaaaagga tagtaagaac acaaataatt taattgatgt atctagtggt tcagatttaa
    33301 ttgcacatgt tggatttgaa atgaatgagg agtcaaaaga tataaacttt gtaatagatg
    33361 agaaaaaaga agatttagaa aaaggtgtat ttccatcaga aggaagtgat aaagaaattg
    33421 aatatgaaaa attagatatg aataatttta caaggacaag atataaagta gataaaagta
    33481 cagatgatta ttatgcaaat atactagcat caaaaagata taaaaaatac ttatatgtat
    33541 ctgtacttta tgctacaagt gattcatcag aaaataacaa agaatattca agtactatag
    33601 atataataaa aattaatcca gaaaataaaa aaatcaagac tataaaaagt ataaatttaa
    33661 gtaattattt ttccgaaaaa taccaaaaca tagagaccat aggtgtaagt tatgctagca
    33721 aatatgagga taagatgtat attttagtaa atgtactaaa tacaaacata ggtgataata
    33781 gtaattctaa atttcattta cttgagtata atcttgaaga aaataaggaa agcataatgg
    33841 aattaaaaac tgatagcaaa atggctttag caaaagcaaa gttagaagct aataaattat
    33901 atatggtaat gtcgaatgat tatgataata gttcaattac agagttaaat ttacttacct
    33961 ataatatatt agataaaagc ttagatgaag ataaatatgt tttagataat agagaaaaaa
    34021 gaggaattag aagtattgaa attgattcaa ataaaatcgt tgcatataca tcaaggctaa
    34081 actctatatt atcttgggat aataaattta tgctatatat aataaataaa aattctaagg
    34141 aaattgtata tgaaggagaa ttacaattta acagttcaaa tagtatgtta gaatttgaaa
    34201 taaagtaggt gagttgatga aagatttaat aagattattc gaatttgaac taaaaagaaa
    34261 tataaagaat tatatattta taataatttg ttgttgttct tttgtaattt taaacatagt
    34321 aaaaaattta aatgactata attacataat agaaaatgca gttaaaagtg aacagttaac
    34381 aaaaatcgga aatacgatag aaacggtaaa ggttacaggt ttctcaagct ttagaaatat
    34441 aatgggttct acagaatcgt ggtttatgtt tggtataatt gcatgtattt gctattcttt
    34501 ttttatttgg tatagagact ttaatggtag gagtaaatct atatatacac ttataatgct
    34561 acctaaaaat agaatcaata tttatatatc taaattatta aatatattat ttctagtgta
    34621 tagctataca gtagtactta caatatcttt gtttattgca agtaagttgt tgcctagaca
    34681 tatgcttggt aatgtcacaa actatggttt tgtacaagaa acaatatatg agttaaaaat
    34741 gttacttcca tatagctttg aaatactatt tgtagagtat atatttttat tgataggatt
    34801 tgtatctgta gtatttacat caattcttat aaataaatct atttataaag taagcacttt
    34861 aataagtttt ctatttttag ttatagaaat tttagtgttt ataatttcta tagaatttat
    34921 tataccctac gaatacagtg atgtatttac tgttctatat tcaagtataa acatatttgt
    34981 atgtagtctt atatctaata aacttttgaa gcaaaagatt gacttttagg agggatatta
    35041 tgaaagcaaa aaatatagga aaatatacaa tatgttttat aatagtatgt tttttaatga
    35101 ttactatgac cttaatattg agttatttta ataaggataa actatctgta aaaactttaa
    35161 aaggcaatgt aaaagatata gggagagtaa gtgtagtata tacacctaat ataagcaaat
    35221 tttcttatga ggaggtaatt ataagtaaaa atggagtaag aaataataca aacccaaaat
    35281 caagaattag aaatgattat ttaaatattg acaaaaaata cagtgacttt gttaatgata
    35341 aaggaaaaga atttatatat aaagatgatt tgaatatagg agaagcgtat acctctgtta
    35401 agtctgattt agtacaagga atgaatgctt taaatgattg tataaaggtt tcaaataaaa
    35461 atttaaaaag tggggtagaa gaaaattttg atattaattt aaataagtct gattttaaag
    35521 aaaatagttg gaaaacctta tttaacaaaa tatataaagg taatttatat atggtaatta
    35581 gtgaaactaa tatttcaggt tctaaagtca ataatatttg tatactaaag atagata
//
LOCUS       DAMJYC010000035        34828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJYC010000035 DAMJYC010000000
VERSION     DAMJYC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.036"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            53..790
                     /gene="cas6"
                     /locus_tag="PC472_001856"
     CDS             53..790
                     /gene="cas6"
                     /locus_tag="PC472_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2727182.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            795..2270
                     /locus_tag="PC472_001857"
     CDS             795..2270
                     /locus_tag="PC472_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727183.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2290..3306
                     /gene="cas7i"
                     /locus_tag="PC472_001858"
     CDS             2290..3306
                     /gene="cas7i"
                     /locus_tag="PC472_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2727184.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3322..4119
                     /gene="cas5"
                     /locus_tag="PC472_001859"
     CDS             3322..4119
                     /gene="cas5"
                     /locus_tag="PC472_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2727185.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4146..6554
                     /locus_tag="PC472_001860"
     CDS             4146..6554
                     /locus_tag="PC472_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2727186.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6995..7942
                     /gene="prmA"
                     /locus_tag="PC472_001861"
     CDS             6995..7942
                     /gene="prmA"
                     /locus_tag="PC472_001861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2727187.1"
                     /translation="MNNWIEVTIKTTTESVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKGFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7961..8719
                     /locus_tag="PC472_001862"
     CDS             7961..8719
                     /locus_tag="PC472_001862"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2727188.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8721..10019
                     /gene="mtaB"
                     /locus_tag="PC472_001863"
     CDS             8721..10019
                     /gene="mtaB"
                     /locus_tag="PC472_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2727189.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10062..10412
                     /locus_tag="PC472_001864"
     CDS             10062..10412
                     /locus_tag="PC472_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2727190.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10575..10754
                     /locus_tag="PC472_001865"
     CDS             10575..10754
                     /locus_tag="PC472_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2727191.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10784..11227
                     /locus_tag="PC472_001866"
     CDS             10784..11227
                     /locus_tag="PC472_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2727192.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11338..11835
                     /locus_tag="PC472_001867"
     CDS             11338..11835
                     /locus_tag="PC472_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2727193.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12028..12591
                     /gene="raiA"
                     /locus_tag="PC472_001868"
     CDS             12028..12591
                     /gene="raiA"
                     /locus_tag="PC472_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2727194.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12804..13040
                     /locus_tag="PC472_001869"
     CDS             12804..13040
                     /locus_tag="PC472_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2727195.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13053..14396
                     /gene="yqfD"
                     /locus_tag="PC472_001870"
     CDS             13053..14396
                     /gene="yqfD"
                     /locus_tag="PC472_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2727196.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14335..15450
                     /locus_tag="PC472_001871"
     CDS             14335..15450
                     /locus_tag="PC472_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2727197.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15507..15968
                     /gene="ybeY"
                     /locus_tag="PC472_001872"
     CDS             15507..15968
                     /gene="ybeY"
                     /locus_tag="PC472_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2727198.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15974..16693
                     /locus_tag="PC472_001873"
     CDS             15974..16693
                     /locus_tag="PC472_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2727199.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16812..17213
                     /locus_tag="PC472_001874"
     CDS             16812..17213
                     /locus_tag="PC472_001874"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2727200.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17277..18170
                     /gene="era"
                     /locus_tag="PC472_001875"
     CDS             17277..18170
                     /gene="era"
                     /locus_tag="PC472_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2727201.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18182..19561
                     /gene="mgtE"
                     /locus_tag="PC472_001876"
     CDS             18182..19561
                     /gene="mgtE"
                     /locus_tag="PC472_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2727202.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19634..19789
                     /locus_tag="PC472_001877"
     CDS             19634..19789
                     /locus_tag="PC472_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2727203.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19755..20528
                     /gene="recO"
                     /locus_tag="PC472_001878"
     CDS             19755..20528
                     /gene="recO"
                     /locus_tag="PC472_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2727204.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20521..21120
                     /locus_tag="PC472_001879"
     CDS             20521..21120
                     /locus_tag="PC472_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2727205.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21280..22158
                     /gene="glyQ"
                     /locus_tag="PC472_001880"
     CDS             21280..22158
                     /gene="glyQ"
                     /locus_tag="PC472_001880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2727206.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22158..24224
                     /locus_tag="PC472_001881"
     CDS             22158..24224
                     /locus_tag="PC472_001881"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2727207.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24390..25937)
                     /locus_tag="PC472_001882"
     CDS             complement(24390..25937)
                     /locus_tag="PC472_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2727208.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26217..26984
                     /locus_tag="PC472_001883"
     CDS             26217..26984
                     /locus_tag="PC472_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2727209.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27267..27464
                     /locus_tag="PC472_001884"
     CDS             27267..27464
                     /locus_tag="PC472_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2727210.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27493..28569
                     /gene="vorB"
                     /locus_tag="PC472_001885"
     CDS             27493..28569
                     /gene="vorB"
                     /locus_tag="PC472_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2727211.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28572..29291
                     /locus_tag="PC472_001886"
     CDS             28572..29291
                     /locus_tag="PC472_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2727212.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29304..29843
                     /locus_tag="PC472_001887"
     CDS             29304..29843
                     /locus_tag="PC472_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2727213.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30000..31058
                     /gene="buk"
                     /locus_tag="PC472_001888"
     CDS             30000..31058
                     /gene="buk"
                     /locus_tag="PC472_001888"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2727214.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31076..31969
                     /locus_tag="PC472_001889"
     CDS             31076..31969
                     /locus_tag="PC472_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2727215.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31999..33207
                     /locus_tag="PC472_001890"
     CDS             31999..33207
                     /locus_tag="PC472_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2727216.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33231..34490
                     /locus_tag="PC472_001891"
     CDS             33231..34490
                     /locus_tag="PC472_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727217.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14059 a         3535 c         6451 g        10783 t
ORIGIN      
        1 tatattacaa atagagaata atgaattata ctagaaagta ggaggtgaaa atatgagcag
       61 aatttcaatt tttatggaat catgtaatgg caaatttcca aatgaaaatt caatgttgtc
      121 agtttccttt ataaaaaata ttttaaatgc agaaaacaaa gattttgcta aaaacatatt
      181 taactatggg gaagaaagaa aaaacaataa gcaaataaag gatataaata cagccattta
      241 cataccaaat cttataagaa gcaaaaatga gttacttgtt gatggagata taatattcaa
      301 tataagtttt tataattatt ctatgttcag taaattatac aatggaattt taaaagtaaa
      361 ggagctagaa tataaaggtt atagatttaa aataaaaaat ataaaaatac ataaagaaca
      421 cgagataaaa gaaaatggtg ttatatttaa aactatgtct cctataatag taaagaataa
      481 agaaggtaaa tatttagatg tagaagattc aaattatata gaatgtttaa actatatagc
      541 taatctaact ctggagagta taagaggtag tgggcttaga aagccattgg aatttatacc
      601 tcttaacttc aaaaaaagag tgttaaaaga aaagattcgt ggatttaaag agagagaatt
      661 ttactatata aatgcttatg caggaacatt ttttttaaaa ggaaatatgg aagatttaaa
      721 tgcactgtat aaaatgggaa ttggatatag aaggacggaa aatgcaggaa tggttgatat
      781 attaaagtag cgaggtgaga gaaataaaag ctaaatttta tagatcaggt gatattgtaa
      841 gagatattgg cattgtttac ttttgtgaat tactaatgga tttaaaaaac gaactagaaa
      901 aaaatgatta taagctaaaa tttacttgtg agttgaatag aaattattta tctttagaat
      961 ctgcagaata tatagaccct aagtatatat ctgattatat attagaaaat caacttttta
     1021 aggtatttaa aaatgggaaa aaggaaacct tagaaaaaat atttagtaat atagataatt
     1081 taacatatgt aaattttatt gaagaattgg ataaatcttc tgcaactgag aaacaaaagg
     1141 aagggattaa aaaagatttt aaaaacagaa gatttccata tgttagaaat agtggtaagt
     1201 atggattaaa cacgagtcta gaaaatatgg aaacaaatgt aaaaagaata gtcgatattg
     1261 tgattaaatc aaatatagag atagatgaaa tagataaact tgatttgaaa caatatgaag
     1321 aaaaagatag tgtatgtaat gtttgcaata taaataaaac aacaaaacta gatattgatt
     1381 taagagaacg aaaggactct aagtataact ttttatttag aggtgcagaa aaatcaggtt
     1441 ttaaaagaag tggacaagtt gaaagtaata tttgttttga gtgtgaattt ttcaacttaa
     1501 tgtgtctttt atatataaat ttaaaaagac caatggtttt tgcatatacg aatgatttaa
     1561 gagaattagc atttttaaat cataagataa tgcttaaaag aaaaatgtat tcagataaat
     1621 cattttataa aaaacttttg catgaaaaaa taagttcaat tagactatat aggattgata
     1681 ttgatacgaa taaaggtata attctaaaat ttgactctat tatagagtat aaagaattat
     1741 taaaaaaaat tgaacttata gatattatag ataattataa tttttcaaga gagccaatga
     1801 atactagaaa tttgggtaaa aaaatgatta ataatgggaa tttatcaaat ttaaaagaat
     1861 tattacttga taatataagt gttttaaggc aactaactgg aacttctcga gaaatggatt
     1921 ttgtatctag ttcatataat atcggtttat atataaaatt gtgttggatt gtagatggaa
     1981 gggaggagta taaaatgaaa aactatatag aaagtaattt aatatattca agagtaggta
     2041 aagacttatt taataaaatg acagatgaat ccagaagaaa aaatttttct atgaaagtga
     2101 ttcaactact taagtctaat gatagaactc agctatttca aactataatg catgtgttag
     2161 tttctaatgg aatcttaata ggagaagggt ttgtagaagg tctaatgcag agttctgaat
     2221 tagaattaaa tacgaatgta ggtctattta ttcaagaaat catgaaataa aaaaggggga
     2281 aaatttaaaa tgaatgaaaa aagagtaaaa ggagtaacat taactatact gacagaaagc
     2341 ccagttccac tttcgtatga ccaagggtat ggaaattata caccaataaa aaaagaacaa
     2401 tatagagata aagtacatgc aaaaactagt atagctacaa taacatatga tttaagaaga
     2461 atactccatc aagaatatgg ttggaattta agtaatattg tttttggaaa aaaaggaaat
     2521 atatatccat ctattaagaa aaatgtagga tgtgtagatg aaaatggatt agagactgat
     2581 gtatttggtt atttaatacc acttaaagag aaaggttcta taagtaaggc ttctccgctt
     2641 agaataatac cttttaggtc attaaattca tatagaggtt caacacagtt gataacaaat
     2701 agaggttttt taagtagcga atttggtaga aaatattatg atgagaggga agaaaatgaa
     2761 gtacttagag atgaaaactt tccaacaaca caagcattag caatagaaga gactttatca
     2821 gattactacg tttatacaat aacattggag cttgatagaa taggagtagt agaagtagaa
     2881 gaagatggaa agttactttt accagaagaa agaaaattta tgagtaagga gttaagagaa
     2941 aaagcagtaa aagatatatt agatgcaatt actgtgttta caagaaacat aaaacattca
     3001 tcagtactat taaaaccact tgcagtaatg ggtggagctt ttgataaagt tgtaccattt
     3061 ttttgggatg atgtagatta taatgctgat agtggtgaga taaatttaga gggagtcata
     3121 gaaactatag aaagttatag ccttaaagaa agtaacacaa tacttgctat caatgacaga
     3181 ttaaaaattt ctaataaaaa ggaattaaat aagtttaatt taagtaaata ccctgtaaaa
     3241 gagattaaaa atctagcaga tagattggaa attggagaag ataatatgtg gtatttaaaa
     3301 gagtaggagg ttttttgaat tatgaaagta ttacaatgta aacttaagca accaactgct
     3361 cactatagag acccaaaagt atttcaaaat gaatatataa gtactttaaa tttaccatct
     3421 aagaccacaa taatgggaat gataacgtat ttatgtgata gaagattaaa ctctgatata
     3481 gatataggaa taattggaac acatcatcat agagaactag aattttcaag aggagagaac
     3541 attgattttt ggaatgaata tacaaatatg agaaaaggca aagataaaga aaagtttttg
     3601 cttcaaggaa actactatga ttactataaa gaacataaag ctcaaaatag catattaaac
     3661 tatgaagttc ttaaagaagt agaactgact atatttattt cttgcaagga tgatgaagaa
     3721 cttgaattta ttagaaaaaa gcttgaaagt ccatgtaaat atgcaaattt aggaagaaaa
     3781 gaagattttg taataccaag tgaaaaaggg tgttttgtga aagaagttga gttaaaagag
     3841 gttataccta tgaatactcg tgatgctata aaagaaaata taaaattaaa gaatacatat
     3901 gtaagagttg acttaagaga taaagagaat gtcgagacta taataaatca aggtgtattg
     3961 attgcattac cttataaata taaagattta gaagcaaata gagatgatag agtttatgag
     4021 ttttgccatt atatatatgt tgataatgat ggtatatatc ctaaaaatat taaggtaaat
     4081 gtatgtattg atacaaaaga agtttttaca tggctttaaa ttattttgaa agataggtgt
     4141 ttatcatgaa aaatatcatg caaaaagaaa tattagctaa gtcatataat ttaagaacta
     4201 atcaacaaga gcaaacatta gttgaacata ttaaggattt atttgaagta ttagaatcta
     4261 tattagaact aaatctatat tcagataaag atgtagagat actaaaaata tgttgtgctt
     4321 tgcacgattt aggaaaaatt aattcaatta tgcaacaaaa aatggagatt aataataaaa
     4381 tatcgtatag ttgctcagaa gaagagagga aaaagttaga gtctgacaaa aaatctttaa
     4441 aaaagatagc aagacataat atattttctg gagcattttt aaaagatata ttagaaaaaa
     4501 tgaacttgtc agaagaagat aagttatata tatataaaag tataatgtta catcatggaa
     4561 actatgaaga ttatatgagg ttaagtatta gtaaagttca agaagaaatt tatgagtata
     4621 ttgaaaaagg cattttagaa aaagaagagt ttaatctgaa agatatagaa agttatataa
     4681 atagcatttt aaatgtagac tttaaatttg gtgaagatgt attggattat gattacatag
     4741 ataaattaag tgaaagtatg tttatagaca gtgattataa caatggtcaa atagatgatt
     4801 cagttttaaa ttatagaaag tttaagtata ttttatataa agggatgctg aacttaatag
     4861 accatagtgc atcaagtaga cagaaaggta taaaatttta taacgatttt actgatgaag
     4921 aaattgataa tatgatttta aatgagatat ataagagtca gggtaatttg gaaaaaaata
     4981 atatagagtt caatactata caaaaaaggt tgagagaact ttctggaaga aatgttctga
     5041 ctgttgcatt tacaggttca ggaaaaacag ctgcagatta tagaaagact tttaaaagaa
     5101 aattcttttt agtgccaaat aaaatatcag cagaaagttt ttatagaaaa aatatttttc
     5161 aaaataaaaa tgatttagat acaagaagta gtaatgatta cattggatta cttcatggag
     5221 atataaactt atattcagag aatgaagata atggagaaca tgattttgta ttgacattaa
     5281 gggatataga tttgagtata aacttttgta aaccttgtgt aattgcaaca gttgaccagt
     5341 tattacttag tatgtttaaa tttccgggat atgaaaaaat attcgcagcc gtaaaaaatg
     5401 catctataac tgttgatgaa gtacatttat tggaaccaaa gatgtttttg attttgatat
     5461 attttatgca gtttgcatgc aagtacttag atgttgattt tcacttaatg acagctacac
     5521 ttccaaaatc atataaagag caaatgataa acaagggaat tatttttcaa gaagaaagta
     5581 atgaaaatgt tactgataaa ggagaaatag tttttattga gagcaacaag gaagaagaga
     5641 tttgtgaagg taaagatgta aaggtttctt ttataaagga aaaagaaatc aagtcaatag
     5701 ttgaagatgc tttagaaaat aaacaaaaaa ttcttataat aaagaatact attgattcag
     5761 tcaatagaac atatgagttt ttaaaagaaa atttatctga taagtatggt ggtgtagata
     5821 tagatgtttt acatagtaga tttaagttca aagataaaaa agagaaatat agtaaaatat
     5881 taaatggaga gggtgatatt tggatatcaa ctcaatcagt agaaatatcc ttggatttgg
     5941 atttcaatat aataataagt gatttagcaa ctatggatag cttgatacaa agaatgggta
     6001 gatgtaatag aaataataaa tatgaatatg gaaactttta tattctacct agtgaagata
     6061 aaatttatga taacaaactt aagaacacaa ctaaaaacat cttaaaggac atattaaaaa
     6121 ctaactctat ttttacaatg gggattagaa aaactatttt ggatgattac tatgataata
     6181 gtgtagttaa aaaatatttt gaagagaatt ttataagttg tgataaagaa attaagaata
     6241 tttatggtat aaataaagaa atttttgatg gattggattt aatttttaat tttgaacctt
     6301 acaaaaatat tgttgatagt aagaaggaag cagctaaaat ttttagagat gttgatgtta
     6361 gttacaaaat tattctggaa gaagattttt ataaggaaga tagggagtta caacaagatt
     6421 cgattcaagt atcagggttt atatttaata gattatatta ttatggatta ataagcaaag
     6481 tagaaggata catggtacta aaatctagta gtaagtttga gtataatagt actgtaggct
     6541 taattttgaa gtgagcatat gtttaaatat aatatacctt tagacattga aaatacaaaa
     6601 agtactactg taagtctact gtaaaaacta ttttttaaaa ggaaaaataa gttgtgagat
     6661 agagtgttta caagtgtata tagataataa atttgaggtt taagattaac taagtgggtt
     6721 tcaggtacat cgcgaaaata attataagtt ggagattttg tatgtttaag attaactaag
     6781 tggatttcaa atattgatat agtagatata atagaagttt tattattagg tttaaaatta
     6841 actaatcgga tttttaaata ttctgactat tatataagga tattattaga ttgaatttaa
     6901 aaccaataat ataaaacaac aaaatattat atttatgata taataaaagt tatggaaaaa
     6961 caggaatatt actatagaga agagaggtaa acttatgaat aattggatag aagtaactat
     7021 aaaaacaaca acagaatcag ttgaacctat aacgaatata ctatatgaac aaggagctgg
     7081 aggagctgta atagaagacc caaaagactt tttattccaa aagaaaaatg aactagactg
     7141 ggattatgtt gaagaagaag tatttaaaaa gaatgaagaa gatgatgttc ttataaaaac
     7201 ttatgtttct gaagaaaaaa atgtgatgga atttgtcgaa ataataaagc aaaaagtttt
     7261 aggattaaaa ggttttggta tagatatagg tgaaggaagt gtatcattgg accaagtaaa
     7321 tgaagctgat tgggcaaatg catggaaagc atactacaaa ccaacaaaag tgggacaaag
     7381 ggtagtagta aaacctacat gggaagatta tgctatgcaa gagggggatt taatcataga
     7441 gcttgaccct ggaatggcat ttggtacagg gacacatgag actactagta tgtgcattag
     7501 agaattagaa aagtatgtga ataaagattc taaagtattt gatattggat gtggtagtgg
     7561 aatccttgca atagcagcag ccaaattagg agcaaaagaa gttgtagctg tagatttaga
     7621 tgaagtggct gtaaaagttg caaaagaaaa tgttcttgaa aataaagttg aaaaaagtgt
     7681 atctgtaatg catggtaatt taaccgatgt aataaaagat aaagcggatg taatagttgc
     7741 aaatataata gctgatataa taaagatact tgcaaaagat gttcaaaact ttatgaaaga
     7801 agatgctata ttcatatctt caggtataat tttggataaa gtagaagaag ttaaagaaag
     7861 cttaattgaa aatggatttg aaatagttga agttcaaaaa ttaggtgaat ggtcagcaat
     7921 agtttctaag cttaaaaaat aagggatatg gtgacgtata atggatagat tttttgttga
     7981 aaaaaataat ataaatctac aagataaaac ttgcactata gaaggtgaag atgtaaaaca
     8041 tatttcaaaa gttttaagat gcaaattagg tgagaaactt gaaatatgtg acaagaataa
     8101 taatgagtat atttgtgaga taatgaatat agataagagt atagttaatt tagagatttt
     8161 agaaaaagtt gatataaata gagaatcaga acttaaagta aggctatacc agggacttcc
     8221 aaaagctcca aaaatggaaa tgatacttca aaaattaact gaagtgggtg tagaagaaat
     8281 aatattagtt caaactaaga gaagtgttgt taaagttgat gacaaaaaag aagataaaaa
     8341 atttgaacgt tgggaaagaa taatttatga agcagcaaag caaagcaaaa gaggtaagat
     8401 accaaaatta agaggtgttt taagttttaa agaagcttta gaagatatga aaaaaaataa
     8461 tgtaaatatt tgcccttatg aaaatgaaag aacagtttca ataaaacatg cactaaaaaa
     8521 atgtgattct aatatagata gtgttggaat ttttataggt ccagaaggtg gattttctga
     8581 agaagaaata gaacaaatac aaaaaaataa ttgtaatgtt gtatcattgg ggcctagaat
     8641 attaagaact gagacagctt ctgttgtagc atcaacaata gctttgtatg aactaagtga
     8701 tttaggagga gaaaaataaa ttgaaaaaag tagcttttta tacacttggg tgtaaagtta
     8761 atcaatatga aacagaagct atgctagaac tatttgagaa agatggttat gaacaagtca
     8821 atagtgaaga gtatgctgat gtttatgtta taaatacatg tacagttact catatgagtg
     8881 atagaaaatc tcgtcaatat ataagacgag ttaaaaagaa gaatccagat gcaataatag
     8941 ccgttgtagg atgttattct caggtatctc cggaagaaat tttggatata gaagaagtaa
     9001 atttagtcat gggtacaaat gatagaagaa agattgttga agaaattaaa aagataaatt
     9061 caagtaagaa agttagtaca gttgacgaca taatgaaagt taaagctttt gaagaaatag
     9121 aaatcagcca aactaatgga aagactagag catttatgaa aatacaagat ggatgtgaca
     9181 gattttgtac ttattgtatc attccttatg cccgaggacg cgttagaagt agagatattg
     9241 atagtatagt cgatgaagtg aaaaaattgg ctaataatgg ctataaagag gttgtattaa
     9301 caggaataca tgttgcctct tatggaaaag atttaaagga tagggatatt aaacttcttg
     9361 atgtaataaa acaaataaac cagatagaaa aaatagaaag aattaggctt agttctgttg
     9421 aaccaatttt atttacagat gaatttgtaa atgaagtttt aaagatggat aaagtttgtc
     9481 ctcattacca tttatcactt caaagtggat gtgatgaaac tctaaaaaga atgaatagac
     9541 gatacacaac tttagagtac aaaactattg tagatagatt gagaagtaaa atgccagatg
     9601 tggcaataac tacagatgta atagtaggtt ttcctggaga gacaaatgaa gagtttaaaa
     9661 aaacgtacga gtttttaaaa gaaatagagc tttcacaaat gcatattttt aaatattcac
     9721 caagaaaagg aactccagct gcaactatgg aaaatcaagt tgaccctcaa atgaaacact
     9781 ttagaagtga acaactttta aatttaagta aagtaaattt taataaattt gcaactaaat
     9841 ttatcggccg tgagttggat gttttgtttg aacaaaatat agaaggcaat aaatatgagg
     9901 gattaacttc aaattatata agagttgttg ttgaaagtga caaaaatata caaggtcaga
     9961 tattaaaagt gaaaataaat gatgtaaaag atgaatatgt agaaggaatt ttgctataac
    10021 atgtagaaat tataatattt ataagatagg aggtggaaag aatggattgt attttttgta
    10081 aaatagcgaa cggagaaata ccttcaacta aagtttatga agatgataga gtcttagctt
    10141 ttaatgactt gaacccagtt gctccatatc atatattagt tgtacctaaa aaacattatg
    10201 atagtttaat tgatattcct gataaagaga tggacattgt gtcacatatt catgttgtta
    10261 ttaataaaat agctaaagag aaaggttttg accaaactgg attcagagtc ataaataatt
    10321 gtggaagtga tggagggcaa gaagttaaac atttgcacta tcatatttta gctggtaaaa
    10381 agcttcctaa ttacgaagcg ggacaaaact aaactaaatt attgaaaaat tgagttcctt
    10441 cttgataaaa atacttgata aaagtaaaaa tttagtttat aataaacaaa gtgttaggtt
    10501 gtaatgcata ttatgcaaaa agaacatagt tacagtccca gcataattgc gctattggag
    10561 ggagggaaaa agaaatgtca gaagtaagag ttagagagaa tgaaacatta gacagcgctt
    10621 taagaagatt caagcgtcaa tgtgcaatgt ctggcatcat gtcagaagtg agaaaaagag
    10681 agcactatga taagccaagc gttaagcgta agaaaaaagc tgaagcagca agaagaaaaa
    10741 acgctaagaa atagtagtaa ataagaataa agaggtgaag gggatgtccc ttaagcaaaa
    10801 gttacaagag gatttgaaat cttcgatgaa aaacaaagat acagtgagaa aatctgtagt
    10861 aactttaata agagcttcta taaagcagta tgaggttgat aatagggtcg agcttgatga
    10921 agatggaatt atcgatgtaa tagcaaaaca attaaagcaa cgtagagatg cattagttga
    10981 atttgaaaaa gctggaagag aagacttaat aaaagaaaca gaaggtgaaa ttgaagtttt
    11041 aaaagagtac ctacctcaac aattaagcga agaagagtta gaagaaattg taaaatctac
    11101 tatatctgaa gtaggggcaa cttctatgaa ggatatggga aaaataatgt cagttatcca
    11161 accaaaagtt aaaggaagag ctgacggaaa acttataaat aagttggtaa aacaaaactt
    11221 acaatagatt gatatagcct agagagaaat ctctaggctt tttatagttt ttgggaataa
    11281 cgagtcatat ttgagcatat ctataatcaa agaaaatttt gaaagaaggg gaaaaatttg
    11341 attatatcag taaaagagat taggaatttt ttgataaaca tatttatacc actaataata
    11401 ggttacttaa gtagcatctt aacaatgatt atcagtggaa cagatatttc aacctattat
    11461 ttacaactca caaagccaag ctttgcacca ccagctttta tctttcctat agtttggact
    11521 atattgtaca tattgatggg tatatcttca tacctaatat taagaaaagg ttacaactta
    11581 cctaaagtaa gagatgcaat tttttattat ggattacaac ttgtattaaa ctttatttgg
    11641 agtatactat tttttggatt tggacttaga tttacagctt tgatagatat aataataatg
    11701 attttggttt ctataataat gatttcgaag ttttctaaaa tagataagag atctggatac
    11761 ataaatttga tttatttaat gtggcttgtt tatgcaggat ttttgaacta ttttatatgg
    11821 tttattaata aatagaatgt aaagattttg taaattaccc tgtactttta ataatatcat
    11881 aacaaacgaa aaataaatag actattaaaa ataatcattt tcatatacaa taatattaat
    11941 ttaaatacaa taaatttata aaaattgtat ttaaagatta acatattaaa gtattagaat
    12001 tggaaaggct tgaagggaga ggtttttatg caaataatag tatctgggag acaaatgaaa
    12061 ttaactgacg gaataaaagg atacgtagat ggaaaattaa gtaggttaga aaaatacctt
    12121 gatcctgaat cagaagtgaa agtaacagta agtgcgaaaa aggatagaca aaaagtggag
    12181 gttacaataa ttcctattaa cggtcaaata ataagagcag aagatgtaga agatgactta
    12241 tatgcagcaa ttgatattgt ttgtgacaaa ttaagtagac aagttgtcaa atataaaact
    12301 aaagttaaag acaaagttca aaataataag agcataagat ttgaaaatct tgactttata
    12361 gataatagca gtgaatttga tgattatgat tatgatgatg aagaagatga aaatatagta
    12421 atagaaagaa gaaagaaatt taatgtaaaa cctatgagtt ctgaggaggc gatacttcaa
    12481 atggaactag taggacacaa tttctatatg tttagaaacc aagataattt tgaaataaac
    12541 atagtgtata agcgtaaagc tggtggatat ggtttaatag aacaagatta ataaaaattg
    12601 tatacgttta gattttatat aagaaaaaat aaaaaggtaa tcatgttgat tttttatcaa
    12661 tatgattacc tttttcttgt ttaaataata tattagatat taggtattta ttatataaaa
    12721 attttaaaac taatctgtta caacatcaat aaagtgcata aatacctatt tcaagcataa
    12781 aattaattaa gggggtaatg cttatgttag aaatatccac tgatttacaa gtaaatcaac
    12841 cagttattac tgttacaagt aatacgttta ttagtataga aaattatcta tcaatattag
    12901 aatatgaagt tgatttaata agaataaaga ctaaagttaa aacaattaaa atatctgggg
    12961 ataaactttc acttaagtat attactgata gtgagatagg tataaaaggg attatctaca
    13021 atgttgagta tgttgattag gaggagatac acttgataag ttttataagg ggatactatg
    13081 ttatagttgt ggaaggagta ggactcgaac aatttttaaa tcatcttata agaaatggga
    13141 taagtgttta caatgtaact agaataaaaa atactaaaat ggagtttcat atagatagac
    13201 aagatataaa agactttaag aatgtttata gaggaagtaa gtttgatata aaggtaaagc
    13261 aaaaaactgg ggtccctttt attatcaaaa gagtttataa gcacaaaggt atgtggattt
    13321 gtgctcttgt ttctttattt ttattgatgt ctacatcgca gtttgtgaca gatgtatata
    13381 tacaatctcc tgaaggtata aaaaaagaag ctcttagaaa tgagctttat aaggttggag
    13441 taaggccagg agtctataag aagagtattg atagaaaaga agttagagac catatgatgt
    13501 caaaatttaa tgatgtggca tatttatcta tcaatgtaaa aggaacaaat atatttgtta
    13561 ctgttacaaa aaaggcagaa tccctaaaat ctacagacca atcaaattat tgtaatgtaa
    13621 ttgctttaaa aaatggtata atagaaaagg taatccctag aagtggtaaa tcagttatta
    13681 aatctggaga catagtccaa aagggagatg tcttattaaa tggagctaat accaagtcaa
    13741 taccagaagt atgggcaagt actttttatg agtccacaaa aaaggcaagt tatgtagata
    13801 ctgtaaataa aaaaactgga gaaaagaaaa atatatatac actaagtttt tatgataaag
    13861 agtttacaat gagaaaaaat ataaaatata aagattatgt tgttgaaaat aaagaaaaaa
    13921 aactatcaat aggaaactat acttttccaa taaagataaa aacgagtact ttttatgaaa
    13981 caaaaaaggt tgaagtaaaa aggaataaag aagagttaaa aaaagaatta tctgaaaaag
    14041 ctttaaaaga gttagaatac ataatcccag cttcggctag aataatagat gtaaaacata
    14101 actttaaggt taataaaaat atgttagaat atttaataac agttcaaact agtgaaaata
    14161 ttgctaaaat ttatcctctt agtaagtcag aagcagaaag gtttataaag gaagaaagta
    14221 aaccagatga aggtgaagag gaagttcctt ccaatccaga aaaaagacca ttaaatgata
    14281 taagaaatga gtttgatgag gataataagg ataataaaga ccaaaacaat tctgatgaaa
    14341 ataatagtaa tcaaaatagt aataatagtc aaaataataa tagcaataac aactaataaa
    14401 attaaactta aaatcttagg aggactaatt caattgataa tacaaaagaa atttacaata
    14461 gcagatggta actttgaaag agagttattt ggcaactttg atgaaaatgt aaaattaata
    14521 gagaagactt taaatataga tgtaatactt agagaaggca atataatttt aattggagaa
    14581 gaaaaaaatg tagattctgc attaaaactt atgaatgaat tacaccaaac tgtatcaaat
    14641 ggtaagcatt tagataagca gagtatttct tattcattat ctcttttgct tgaaggaagt
    14701 gaacaaaaaa taaaagaact tgaggggaca atcgtcataa ctcaaagggg aaaagctgtt
    14761 caacctaaaa ctttaggtca aaaagaatat ataaaactta tagaaaataa tgatataact
    14821 tttggtgttg gacctgctgg aacaggaaag acttatcttg cggttgctat ggcagtaaaa
    14881 gcttttaaaa gagatgaagt tagtagaatt atacttacaa gaccagctgt ggaagcagga
    14941 gagagtctag gttttttacc aggagattta aaggacaaag ttgacccata tttaagacca
    15001 ctatatgatg ccttatttga aatgttgggt gctgataaat ttaataagta tttagaaaga
    15061 ggtactattg aggttgcacc acttgcattt atgagaggta gaacacttga taattcattt
    15121 ataatattgg atgaagctca aaatactact tctgaacaaa tgaaaatgtt tttaactaga
    15181 cttggatttg gctctaaagc tgtagttaca ggagatgtaa ctcaaacaga tttaccacaa
    15241 aataagaaga gtggtctaat acaagctaca gagattctta aaggtgttcc aggtattggg
    15301 tctataatgc taacagatag agatgttgta agacatgagt tggtacagag aataataaga
    15361 gcttatgaga agcacgacaa aagagaagaa tttaaaaaag aagaacgtaa aaaagcgaaa
    15421 atgcaggaaa agactttcaa aagaaagtaa aagtgtagta atatagatat atagcattat
    15481 taatgaataa aaagtaggtg tagtgaatgg atttgatact agatgataga caagataaat
    15541 tagaagtaag tgaggagtta attgaaaaga taaaggatat aatcatagaa tgtttagatt
    15601 atgaaggata tgatgataat tatgaagtga gtctttcttt tgtagataat aaagagattc
    15661 atgaattaaa tagagaatat agaggagttg atagagtgac tgatgtttta tcatttcctc
    15721 ttttaagtga tgattttgag gatgttgagt tagaagaaga atccctaggt gacatagtag
    15781 tatctcttga aagagcctta gaacagagta tagagtataa ccatagtttt gaaagagaag
    15841 tatgtttttt aatatgtcat agtatgtttc atcttttagg ttatgaccac gatacagatg
    15901 aaaatacaaa agaaatgaga gaaaaagaag aacacatatt aaataagtta aacataacaa
    15961 gggagtagtt tttatgaaac cagaaaaaac tcgtcaaggt ataattaaag catttaatgc
    16021 agctatagaa gggatattgt atacatttaa atttgaaaga aatatgaaaa tacattattt
    16081 aggttcagtt gctgttctta taataagttt gttttttaac ttttcaaaat tagaaatgat
    16141 aatgttactt atgtccatat gtttagttgt agttgctgaa atgtttaata cagctataga
    16201 aaaagctgtt gatttagtaa ctgatgaata tcatgttctt gctaagatag caaaagatgt
    16261 tgcagctgga ggcgttctag tggcagcact aaattcagta gtagttggat atattttgtt
    16321 ttatgataag ttgacagata tatctggaat attaatttat aaaataagag aatcagaatt
    16381 acatataacc ttgatatgta tattattggt tcttatagct gttgtagttg taaaagcatt
    16441 gacttctaca ggtactcctc taaaaggagg aatgccaagt ggtcatgctg ccttagcatt
    16501 tgctatagca actgcaatta cacttatgac tgaaagagta gttgcctcta ctcttgcata
    16561 tataatggct gtattagttg cccaaagtag aattgaagga aaaatacata ccttttggga
    16621 gactattgct ggagctttgt taggtgtgtt gatagctata ttagtatttc agcttggaat
    16681 gttctataat taaaataaat gtacataaaa tatttaaaat aaaacaaatc tgtgctaatt
    16741 aaatttttat gatataatgt agagtattaa ataaaaataa cttttaatta ttgtatacag
    16801 gaggagttgt tgtggataac atagaattgt taaggttggc agaagatgcc agacaacatt
    16861 cttatgctcc ttactcaggt tttagagtag gtgcagctct tttaactaag agtggtaaag
    16921 tttatactgg atgtaatata gaatgtgcat cattaggtgg aacaaattgt gcagaaagaa
    16981 cagctatttt taaagcaata tcagagggtg ataaggatat ctataaaata gctatagcaa
    17041 gtgataactc agaaaataat gagcaaacat atccatgtgg aatatgcagg caggtaatta
    17101 tagagtttgg ttctgatata aaaataataa cgggatatac taaaggagag gtgtttgagc
    17161 atagtataaa ggatttgttg ccaaactatt tctctggaaa tgatttaaaa taaatttaaa
    17221 agtataaata aatttaagta attaaaacaa gaagataaaa agttaggaga cagttaatgt
    17281 tcaaatcagg ttttgtcagt atagtgggta gaccaaatgt aggaaaatct actttaatga
    17341 ataatgtagt tggagaaaaa atagctataa tgagtgataa gcctcaaact acaagaaata
    17401 caatacaagc agtatataca gatgaagaaa tgcagatagt attcttagat acaccaggta
    17461 ttcacaaacc aaaaaataaa ttaggtgaat ttatggtaaa ggcagcaaca gaagctttta
    17521 aaaatgtaga tttaatatta tttgtagttg atgattctaa aaaaataggt cctggagata
    17581 gaaagataat agaggattta agaagtgtta agacaccaat aattttagtt gtaaacaaaa
    17641 tagaccaact agaccaaaaa gatgagctat ttgatataat caaaatgtat gatagagaag
    17701 gcatttttaa agaaatagta ccaatatcag cattaaaagg aaagaatacg gataccttaa
    17761 taaaagttat acaaaattat ttagaagaag ggccaaagta ttttccagat tatatgatta
    17821 cagaccagcc agaaagagta ttaatagcag aacttataag agaaaaagtt cttcactatc
    17881 ttaatgatga aatacctcat ggtgtagctg ttgaaattga aaaaatgaaa gcgagaaatg
    17941 ataaggaaat agtagatgta tctgctgtaa tatactgtga aagagattca cataagggaa
    18001 taataatagg aaaaaatggt aggaaactaa aaggaatagg taagtcagca agacaagata
    18061 tagaattgct tttaggttca caaataaatc ttcaattatg ggttaaagtc aaagaaaatt
    18121 ggagaaattt acaaaattat ataaataatt ttggatataa tgataaataa aggtgggtta
    18181 tatggatagg aaactggatg cttcccaaga tttattatat gaagtaaaat cattaattga
    18241 taataataaa gtattggagt taagagaatt aatagaggaa tatcatataa ttgatatatt
    18301 cgatataatg gaaaatttag aggaagacat gaaaattcaa ttatttgaag tacttccttt
    18361 agatatggca tcttctatat tagaagaagg tagtgtagag ttttttatta gtattttatc
    18421 taaactggat gtagaacact cgaaaaatat acttgaactt atgtctcttg gagatatggc
    18481 agacaagttg agtgaacttg aagaagaaga aagagaacac ataataaatc ttttgaatca
    18541 agagaatgcc gattatgtaa aagagttact attctatgat gaagattctg ctggtggaac
    18601 aatgaccact ggatatattt ctataaataa agatatgaca gctcttgagg ctatagacca
    18661 tatgagagaa gaagctgaag aagctgaaac tatttattat atatatgtgg ttgatgatga
    18721 agaaaaacta gttggagtat tgtcattaag agaattaata atagctagag acgcaaatat
    18781 agtagaagat ttgatgagtg aaaatataat ctctgtttat gtagatgaag atagagaaga
    18841 agcagtaaga ttagtatcta aatacaattt aatagcaatt ccagtagtag acagacaaga
    18901 aaaattaaaa ggaataataa cagtagatga tataattgat gtaatggaag aagaagcaac
    18961 tgaagatatg tataaatttg caggaagttc agaacatgaa agagaagttg ctgaaaaaga
    19021 aaaccctaca ttgagagagc aaataatatc agcacttaga ggacgattac cttggttaat
    19081 tattacctta gttggagggt tgttggcttc attaatactt tctaacttag actatataat
    19141 gaatcctgta tatgctccat tagtattttt tatacctgta gtaattggta tgggtggaaa
    19201 tattggaact caatcatcat ctgttacagt aataacatta tctaataagg atttaaattt
    19261 tagcaatgtg gttagagaag gtattgtagg tattattaca ggtcttttgt gtagtataat
    19321 aacaggtatt gtaatttact ttgttatgag aaagttagac attgtattaa ttgtttcaat
    19381 atcattattt ataaatatgg ttttaggtgc aacaataggt gcgtttatgc ctgttttatt
    19441 aaaaaaaatg gatgcagacc catctacagt ttcatcacca attatatcta cagctcttga
    19501 tataactggt atagcagttt actttataat aacaacggca ttattgtcaa aaattgttta
    19561 ataactttat gtatgattta tcctcttctt actaaaaata taagtataaa tgtaagaagg
    19621 gggtttttta aaaatgaaca atatcagttg ggaagttttt aaaaaaacag gtagtataga
    19681 tgcatattta tatttctgtg attgcaaaaa catacatgaa gaaattgaag aagtaaggga
    19741 gaaaaaagaa gacgatgata atcctgaaca cccaggggat agtacttaaa gctataaggt
    19801 ataaggaaag tgacataata cttacactat ttacaagaaa gcttggaaaa gtatctgcaa
    19861 tagcaaaagg agctaaaaaa aataagagct ctttgctttc atcatcacag ttattttctt
    19921 atagcaattt taccttaaaa aaacaaggta atatgtacaa ggttactcaa agtgaaataa
    19981 taaaaagttt ttataatata tcctatgata tagaggcatt ttcttatgct acatatatta
    20041 caaaattagt tgaaaattct atacttgaaa atcagactaa taataggcta tttatattac
    20101 ttgcgcagac tctgtatttg tatacacagg ataatacaga caataggttt ataacagcag
    20161 cattcgagtt aaaattttta gactatatag gatttaaacc tatagtaaat aaatgcatta
    20221 attgtgaatg taaaatttta caaaattctg tgtttaatat ctacgaaggt ggtatactat
    20281 gtagtaagtg tagtaaactt tttgataata atataaaatt agacttaaca actataagtc
    20341 ttatggaata tgtattaaga aatgatattt taacatgtag taaagctaaa gtatcaaaat
    20401 atataacaca tgaacttgag aatattctag acaagtattt gaaagtttat gttgataata
    20461 ttaattttaa atcattacac gtattgcaaa gtgtaaaaaa taataaggga gtggataatg
    20521 atgagtagta atataactat tgaaatggtt gattcagtct ttgaaagagt tccaggagca
    20581 acttatgcag aagcaaaaga agcattagta atgtgcaatg gagatgtaat tgaggcagta
    20641 atatatttag aaaataaaaa aaatacttgt aattgtaaag caaaatctgc taaagaaact
    20701 atggaagaag tatttggcaa agatggagaa aatataaagt ttcaattaaa agagcttctt
    20761 agaaaaagta gtgtagtgag aataatagtt gaaaaagatg gaaaaactat gatgaacatt
    20821 cctttaacag ttggagttgt tggattagct tttgtaccat tagctacttt agttggatta
    20881 tcagctgctg ttataagtaa atatcgtata aaagttcaaa atgaagaaga tggagaaatt
    20941 gtagatttag gtgaattgaa tgaagaaaaa cttcacgttt tgaagaatat gataactaat
    21001 gcagcaaaag atgtgaagga tgtagtagtt gacaaaaaag aagatgacaa agatgtaact
    21061 gctgacctta tgaaagaaga atctgaaaag aataaagaag acgaagacga aaataaatag
    21121 atataaagtg acttttaaaa aatgaagaga agataattat aagaattatc actaacattg
    21181 acaaatttta tagaatttga tattctaaaa gttgtagaag aactattaaa atagcctttc
    21241 gtgtagacga gaggctttat tgttatagga gggattttta tgaattttca agagatgata
    21301 ctggcgttgc aaaaatactg gtcaaagcaa ggttgtatca tgatgcaacc ttatgatatt
    21361 gaaaaaggtg cgggaactat gaatcctaat acttttttaa gatcattagg acctgagcct
    21421 tggcaagtat gctatgtaga gccttcaaga agaccagcag atggaagata tggtgaaaac
    21481 ccaaacagac tatatcaaca tcatcaattc caagttatac taaaaccttc tccagataat
    21541 atacaagaat tatatttgga aagtttgaaa gagattggaa ttgacccaag tgagcatgac
    21601 ataagatttg ttgaagataa ctgggaagct gctacagttg gtgcttgggg acttggatgg
    21661 gaagtttggc tagatggtat ggaaataact caatttacgt attttcaaca agtaggtaat
    21721 attgaatgtg agcttgagac aggagaaatt acatatggat tagaaagatt agctatgtac
    21781 attcaagaag ttgatagtgt ttatgattta aaatggaatg ataaaataac ttatggtgaa
    21841 gtatttaata aagctgagta tgaaaactca atgtacgcat ttgaattatg tgatgcagat
    21901 atgttgttta atctgtttga tatatatgaa aaagaagcat taagacttat ggaaaatggc
    21961 cttgtaatac cgtcatatga ctatgtgtta aaatgctctc atgcatttaa tacattggat
    22021 gcaagaggtg caataggagt tagtcaaaga gcttcattta taggtagagt tagaaatatg
    22081 gcaaagactg ttgcagaaac ttttgtaaaa caaagagaag aaatgggatt ccctctttta
    22141 aaggacggtg acaaataatg aaaaattatc ttttatttga aatcggtgtt gaagaaatgc
    22201 catcaagatt tgtaggcagt acactagaac aattaaaaaa caatcttagt aaattatttg
    22261 atgaaaatag aatagctttt gataaaataa atgcttatgg gacaccaaga agacttactt
    22321 tagtagtcga aggattgagt gatagacaaa gtgatttaga agaggaaata aagggtcctg
    22381 ctaaaaaaat tgctgttgat gcagaaaata atttaacaaa accagcatta ggatttataa
    22441 aaagcaaagg attaagcgaa tctgacatat attttaaaca agttggaaaa gaagaatata
    22501 tatttgcgac tataaagcaa gatggaaaag aaacttcaga agtactaaaa gacatattac
    22561 cagagactat aaaatctgta gtattcccaa aagctatgcg ttggggagga aaaaatctta
    22621 aatttgcaag acctataaga tggatagttg cattgttaaa tgataaagta ttagagtttg
    22681 atttagaagg aataatatct tctaatgtaa caaaaggtca tagattttta ggtgaaagta
    22741 caatagaagt tgattctata gaagattatt ttgaaaaatt agaaaaaaat tatatagttt
    22801 tagaccaaaa caagagaaaa gaaatgataa gaaaacaatg tatagaagta gcaaattctt
    22861 taggtggaga agttgaattt gatgaagatt tacttgaaga ggtaacttat ttagttgaat
    22921 atccaactgc tttttatgga gaatttgatg tggattatat aaaactacca aaagaagttg
    22981 ttataactcc tatgaaacaa catcaaagat attttccagt acttaaagaa ggaaaactgt
    23041 tacctaactt tatagctgtt agaaatggtg attcttacag aatagacaat gttaaagctg
    23101 gaaatgaaaa agttttagaa gcaagacttg cagatgcttt atttttctat agagaagata
    23161 ctaagagaaa cttagaaagc tacatagaaa aattaaaaac tgttgtattc caagtaaaat
    23221 taggaactat ttacgacaaa actttaagac ttgaaaaatt aagtgctgat atacttgata
    23281 gtttaggttt agtagatgaa aaggcagata ctataagggc tgctaaatta tctaaggcag
    23341 atttagttac tggaatggta ggagaatttg atgaattaca aggttttatg ggaaaagaat
    23401 acgcaaaagt tggaggagaa aatgatgttg taagtgaagc tatttttgaa cattatcttc
    23461 caagatttgc aggagatata cttcctaaga ctaatgcagg ggttgcctta tcaatagctg
    23521 ataagttgga ttcaatagca ggtttctttg ctataggaat acaaccaaca ggttctcaag
    23581 acccatatgc tttaagaaga caagctttag gtatacttag tatacttatg gacagaaaag
    23641 ttgctgtgga tataaaaata cttattgaac atgctttaga caattattct gaattagatt
    23701 ttaataaaga agaagttgtt gagcaaataa tgaatttctt caatgagaga gtaaaaaatc
    23761 tatttaaaga tttaggaata agatatgatg ttgttgatgc tgttttaagt tctgatataa
    23821 atgatgtttc tgatatgcat atgagagctt tagagcttaa taattggcta gaaaaagatg
    23881 aattagtgga gatgcttact gcatttaata gagtttctac tctagctcaa aaagcagaaa
    23941 gtgataaaat tgatgaatct ttattaaaag aagatgctga aattaaactt tacaatgaat
    24001 ttaaacaagt aaaggtaaaa gttaatgaat tattaaagga taagaaatat ggtattgctt
    24061 tagatgaatt tgcatcttta agaccagtag ttgataattt atttgataca gttatggtca
    24121 tggataatga tgaagctatt aagaacaata gacttgcttt acttaaacaa gtatatgata
    24181 ctatgctagg aatttgtgat ttatctaaaa ttgtttataa ataaaattta aactatttat
    24241 aagaaataag gctgtctcaa aatagagata gtcttatttt tcattgttga gatgatgaaa
    24301 atatatttga tttcatagaa aaagagtgtc tcataaacta aaaagttcat tttgagacac
    24361 ccatgttttt tgtaagtaaa tataataaat tatattttat acttatcagt tattttgttt
    24421 aaaatattat catttgaatt tacccattca taaaaattgc ctgaagatgc gtccgatatt
    24481 ttataaagca tttcaggaat atcactagct ttaaaatctc caagtgattt tcccaatcta
    24541 gtcttaccta tttcaaaact gcctccaaca aataattcaa atgcatctgt agatatacca
    24601 tcaacattct tctttttacc acataaacct atgctaccaa cttgatgaac accacatgaa
    24661 tttaaacatc ctgatatata taacttaggt gctttattta aaatttcttc attattttgt
    24721 aatctaaaat aatctatgat ttcatgtaac attttctgac tattttgtat gcccatttga
    24781 caaattggaa cacctatgca agatatactt tgttctaatt gagaattaca actaatagtt
    24841 ttacttattt ctaatattct ttttgcttca tttccattta aatttaaaat atataatccc
    24901 tctgtcattc ctagtctaat cataggattt tcaacattat ctaattcttt aagtaaagta
    24961 cttaaatcct ttgttaacaa aatacctcca actggatgta tatatacagt gtataaacca
    25021 tcctgttttt gaggtattag tctagaatct tgtatatcta ttttaactcc aggtttagag
    25081 taatctattt cttcaatatt gagttcaaga ctaccatttt ctttttctaa tttatagtat
    25141 tctttaaatt tttctaagaa tagttcttca ccaagtgatt cgaccatata tctaacccta
    25201 gctctgtttt tattttcata atttccataa tccataaaga atttaatcat accttcaaca
    25261 caatataagg cgtctttcgg ctttataagt tcatcaagtt ctaaagcaac tttgggttgt
    25321 ttacctagac ctccacctaa gaaaactctg aaatatggtt catcattttt caaagtagca
    25381 acaaatccta agtcttgtat actacaatga gcagtatctg tgtaacaatt agaataagaa
    25441 acttttaatt ttctaggtaa atggtatgta gtagcatttt tgataaaata tttatcagta
    25501 gcaactgcat atggagtaac atcaaagact tcactagggt caactccaga taatggagat
    25561 aatccgacat ttctaggata attaccacca ccacctctag tgaatatatc attttttatt
    25621 ccttcttcca tgatatcaac aattgaattt atagataaat cgtgtaattg tatagcttgt
    25681 ctagttgtta agtgtatctt atctaagttg tgtttaatag ctatttcata gattttatta
    25741 agttgatact gagataaaac tccagcagat actctaagtc ttatcataaa agattttttg
    25801 tctctttgag cataaacacc atatccacca gaaaatccct tatattgcat tttatttatt
    25861 tctccattta aaaattttga agaatattct ttaaaattag gtagttcatt taacaatatc
    25921 tttttaaaat tatccattat ttcacctcat tgttagtctt gtataaaaat atatctattg
    25981 tataatagat atatatatta ttatctagtc aggataaaaa tataacagaa taattctatt
    26041 aaaagtaaga ataataagga catagtgagt attttgtttt tattttacaa attaagtata
    26101 atatttttgt tttataaaat ataattaaga taaatagata attttgtaat tttaatatat
    26161 aatatttagt agagtgtggg agtagcactt aatatttgca ggaggagttt ttgtatatgg
    26221 gagaaataat aaatgcatta gatagagccc tagacataat tttgctgtta tatcatgaaa
    26281 agagagaaat gggaataact gaaataagta aggctatggg agtatataaa agtactgtac
    26341 atagaacttt ggttactctt gaaaacaaag gttttgttat acaaaatgca gagaatggta
    26401 agtattggct aggaataaat ttatatgcaa taggtatggt tgtgggagaa aaaatgtcat
    26461 tgactgaaat agtaaaacca tatacgaaaa aattaaatca agaatttaac gaagtagtta
    26521 atgtatctat attagaagaa agagctcaag attctccaag aagtatcata atacataaag
    26581 agtatggttc aaatcaatta ctttcagtaa atccatccgt tggttcaagt agtgaatgtt
    26641 attgttcagc agttggtaaa tgtcttatgg catttaatga ttctatagat tttgaaaaat
    26701 atagaaaaac accaatacac aagtatacag aacatacaat tgataattgg gatgatatga
    26761 tgcttttttt agcaaaaata aaagaacaag gatatgctat agatgatgaa gaactagaac
    26821 atggactaac ttgtattgga gcacctatct tagataaaaa taataaagct attgcagcca
    26881 taagtttgtc aggtccaact ataagaatga gagaagggga ctttgagtat aagattaaaa
    26941 gagttataga aactgcaaaa agtatatctg aactttttag atgattttta atctcgataa
    27001 taaaaatagg ctatatttaa attgttattt gtaaatatca gtttaaatgt agcctatttt
    27061 tctttaatct aaaattaaat attcttaaaa tttaaaatgt tattgaaata tataagcgaa
    27121 tgtgttatct tttaattaag caataaaaat agaacaaggt tctatactat agaacaaaat
    27181 tgatgcatat atagtattta tacaaaatat aaatattata tatttaaaat taagtaaaaa
    27241 tattacataa aggggagatt tgagaaatgg aaaaagtcaa gttgagtgtt gaaagatgta
    27301 agggatgcta tctttgtatt gaagcttgca aaaaagatgc aataagtctt tctgactaca
    27361 caaataaaaa aggctttgta actattaaag tcgatgaaga aaaatgtatt caatgtggcg
    27421 cttgttatac aatgtgccct gatttagtat ttgagatatt ataaattatt tttattagag
    27481 gggagaaaag gtatgcaaag tttattaatt aaaggaaatg aagctttagc agaaggtgct
    27541 atacgtggtg gttgcagatt ttatgcaggt tatccaataa cgccacaatc agaaatatta
    27601 gaaatgatgg catggagaca aaaagaagtt ggaggagtat ttatccaagg tgaatcagaa
    27661 atagccagtg taaatatggt tatgggagca agtgcacttg gagcaagagc tatgacttct
    27721 tcatctggtc caggattttc attaaaacaa gaaggtattg catattgggt atcagctggt
    27781 ttgccagcag ttgtagcatg tgtgcaaaga tatggtatag gtgatggatt tataggagct
    27841 ggtcaagata attattggca agcagtaaaa ggcggtggaa atggagatta tcatttaatc
    27901 gtgttagcac ctaatagtgt tcaagaaaat atggatatgg catatgaatc ttttgaattg
    27961 gctgaaaaat atatgcatcc agtacttata ctttctgatg ctacaatagg tcaaatggta
    28021 gaaccttgta tagcaccacc aatgaaagaa tacgatatgg ataaagctcc atgggctgca
    28081 aaaggaacac ctgtaggaga aaaaaataaa gtaataactg atataacata ttttgaagac
    28141 caagatacat ggcaaaaagg gtatatagaa aaaatgagaa aagttcaaga agatgaacaa
    28201 agatgggaag agatagaaat agaagatgct gaagtggtat ttgtggcata cggtatttca
    28261 tcaagaatag cagaaggtgc agttagagat gctagagcaa aaggaatgaa attaggtcta
    28321 ataagaccaa aaacattgtg gccttaccca agaaaagcat ttaagaaatt aagtgattca
    28381 ttgaagggac ttgtaacagt tgaaatgaac atgatggcac agatgaagga agatgtaatt
    28441 atagcatgtg ataatagatt tccagtatat tcagtagcaa cagctcaata tatggcagaa
    28501 gtaaatcaat taatagattt tgctcaacag attatagatg gaaaagctaa gcaagaggag
    28561 gtattttaat tatggctaaa aaagcaccag caataatgat gtgtgaaaat atgttctgtg
    28621 gaggctgtgg acactctata gtcgatagaa ttattggaga agtattaaca gaaatggata
    28681 tatgtcaaga tacaattata tgttcagata taggttgtaa tcatatgttt caatttagta
    28741 tgaatgtaga tgttgtcgtt ccacctcatg gtaaaatggg agcagcaatg gcagctatga
    28801 aaagagttag accagataaa tatatattta caatagctgg agatggtgga gcttatgcaa
    28861 tagggcttgg agaaactatg tcagctgcta ctagaaatga taatgtaata tttcttgtta
    28921 tgaacaataa tatgtttgca atgacaggag ggcaaatggc tccaactact atagaaggtc
    28981 aatatacagc ttctactcca gcgggtagaa attacgcaga tcatggttca acatttgatg
    29041 ttgtaaaagt tatgggaaat ttagatatag catatctagc aagaggaaca ataactaacc
    29101 ctaaagaaat aaatacaaca aaaagataca tcaaaaaagc tctagaaaaa caaaaagaaa
    29161 agaaaggttt ttgcttaata gaagtgcttg taccatgtcc aacaaactgg ggattaaagc
    29221 cagtagatgc tatgaagaga attgaaaatg aacttgttcc aaagtatcca cttggagaat
    29281 ttattgatta gggaggaaat aatatgttga atgaaattat atgtgctggt tttggaggac
    29341 agggagttct tacagcagga aaaattataa tgtatgttgc atataaggca gaaaagaaaa
    29401 ttacatggtt cccatcttat ggaaatgaga tgaggggagg aagtgcaaat tgcaatgttg
    29461 ttgtaagtga tgaaaaaatt gcaaatcctt gtgtaaaaaa tccagatata ctattgggaa
    29521 tgaacagcgt atcagtagat aagtatgaaa gtttaatgaa aaaaaatggt tatatgtttg
    29581 tcaatgaatc actagtaagt aaggatagaa agtacagaga agacattaat gttataaaag
    29641 tacctgttac tgaaatagca caaaatttag gcaatgaaaa agctgctaac atttgcatgt
    29701 taggtgctat ggtaaagagt ttggatttat ttacaaaaga agaatttgaa agtggtatgt
    29761 gtgaatactt tgaaaatcaa ggcaaaggta aattcaattc taaaaatgta gaagcatttg
    29821 aagctggata cgactacgaa tgaatatttt aaatatttaa aataattatt gaaatactta
    29881 tttttatatg ttagtattta attaataaaa tatagaacgt cgttctggac aataggacga
    29941 agtttttagg tcggaataca agaacggcgt tctgtatagt agaactaggg ggtgttaaat
    30001 tgaaagtttt agtaataaat cctggtggta catccacaaa aatttctgtt ttccaagatg
    30061 aaaatgaaat tttgaaaaaa aatataaccc atacaagaga agatttaaaa aatttttcta
    30121 aagtatttga tgaatatgat tatagaaaac aactgatagt agatatttta agtagtgaga
    30181 atcatagtat aaattctttt gacgcagtag ttggtcgtgg tggacttatg aaggctataa
    30241 aaggtggtac ctatactgtg agtgaagaaa tgattgagga tatgagaaat gaaataaatg
    30301 gggaacatgc atcaaattta ggtgcattat tagctaaaac tatagctgat gaaataggag
    30361 tacaatcatt tgtagtagac ccagtttctg tagatgaatt tgatgatgta tcgaggatta
    30421 caggaatatc agacatagaa aaagcaagtt ggttacatgc attaaaccat aaagctgttt
    30481 caagaaaaat tgcacaaaaa ctagggggaa aatacgagga ttataacttt attgtagctc
    30541 atttaggttc aggaatatcg attgtagcac ataaacaagg caaaatgatt gatggaagtg
    30601 gtggtaggtc tgatggtcca ttttctccag aaaggagtgg tggattactt gcatatcctc
    30661 ttatagaatt gtgttactcc ggaaaatata ctcatgatga aatggtcgca aaagtaagtt
    30721 cgattggggg aatgtacgat tatttaggaa caaaggacat gatagaaatt gaaaacagaa
    30781 tcaaggaggg tgatgaaaag gcaaaactta taatggatgc atttatttat caagtagcaa
    30841 aagaaatttc tatgtatgga gcttcacttt cagggaatgt agatagagtt atcttgacag
    30901 gagggatatc tcactcagaa atggtagtca aaggagtagc agaaaaagtt tcatatctag
    30961 ctccaataga gatagtacca ggagagatgg aaatggaggc acttgctcta ggggcactta
    31021 gagtacttaa tggagaagaa atagcaaaaa cttattaata tttaggagaa agagtatgtt
    31081 aaaagatttt aataatttat tagaagaggc taaaaagctt gaacctgtta ttgtaagtgt
    31141 tgctgttgca gaagacaaag aagtattaaa ggctgtgaaa tcagcaacag atttaaacat
    31201 tataaagcca attcttgtag gagacaagga taaaatagaa agtatatcaa gagaaattgg
    31261 attaaaaggt catgaaataa taggttgtca aaatcccgat gaatcagtta aaaaagctgt
    31321 agagatagtt aaaaataagg aagctaaagt tcttatgaag ggattagtaa atacaagtgt
    31381 ctatatgaga gctatattaa ataaagaaac gggtcttaga acaggaagat tattaagttt
    31441 gcttgcagta tatgaattac ctcaatatca taaacttctt tattgtactg atagtggaat
    31501 aaatgtatcg ccaaacttaa atcaaaaaaa agatataatg acaaatgcac ttcttactat
    31561 gaaaagtata ggcatggaaa atcctaaaac tgcgatactt actgcaaatg aaatggtaga
    31621 ccctaagata caatctacag tggatgccaa cctacttgta gagatggaaa aatcgggaga
    31681 aataccaaag tgtatagtgg aaggtccaat agcatttgat gtagcatttg atgcacatgc
    31741 agcagaacat aaaggcatta aaagtaatat tgctggtgat gttgatttat tagtatttcc
    31801 aaatatagaa gcagggaata tattaggaaa gtcatggctt agatttaata aagctaagtg
    31861 ggcaggtata gttttaggag caacaaatcc gataatttta ggttcacgtt ctgacacagc
    31921 tgaaataaaa ataaattcaa tagcattagc gtgcctagca tcacaataag aaattattat
    31981 taaggaggaa aaaatattat gaaatatgca aaaagaatgg atatggtaaa agcttcagct
    32041 ataagagatt ctcaaaagaa aatagctcag aaggtagcaa gtggtggaac tgttatatct
    32101 tttgctgcag ggcttcctga cccaaattta tttccaatag aagagataag tgaaataact
    32161 caaaaagtac ttaatgaaag gggaaaattt gccctagcat atggacctac taaaggtgaa
    32221 gatgagttgt tagatctatt agtaaaaaga atgaaagaag aagaaaatat tgatacaaaa
    32281 gcagaaaata ttataataac tactgggtca caacaaggga tagcattaag tgctatgatt
    32341 ttattagaaa agggagatgt agtagttaca gaaaatccaa gttacttagg tgcaataaat
    32401 gcatttagac cttatgaatg tgatttttta ggagtagata cagatgaaga aggaatggta
    32461 acagatgatt tagacaaatt attatctgaa aattctaaca taaaggtgat atatgtaata
    32521 ccaacatttc aaaaccctac aggaaaaaca tggtctttac aaagaagaaa agattttatg
    32581 gaagtcgtga ataaatatga tgtcatagta atagaggata atccttatgg agaaataaga
    32641 tttacaccag aacctctacc tacacttaaa tctcttgata ctaaagataa agtattgtac
    32701 cttgggtcat tttcaaaggt attatgtcca ggatttagag ttgcttggat gtgtggtaat
    32761 gtagacatca tagataaggc agaactttta aaacaaggta tagatttaca gtcaaatcaa
    32821 ttttcgcaag tacaagtaat agaattttta aagaaataca acttaaatga acatatagaa
    32881 aaaataagag cagagtataa gaaaagatgt ctgctaatgt tagatgctat gaagaaacat
    32941 ttcccagagg aagctaaata tacagaacca gatggaggaa tgtttatatg ggttgaactt
    33001 cctgaaagta taaatgtaga tgatttatta gataaagcca ttgatgctgg agttgcttat
    33061 gtttctggag agtccttctt tgcaaataat ggtccaaaaa atactatgag acttaactat
    33121 acaacaatgt cagaagaaca aatcgttaaa ggtgttgaga tacttgcaga agttataaaa
    33181 aaagaactca attatgcttg tgaataaata taaaaagtga gggtttaaat atgggagaaa
    33241 aagaaaacgg tataaagttt ggaaagttaa tgtttcaact tgttattata gcaatatctt
    33301 gggaattagt ttatattata ccatttatcc aatatacatt atatgatcca atcctaaaag
    33361 cattagaatg tacaaacact caactaggtt ttctacttac aatttatggg ttaggtaata
    33421 tatttggagc tcctatagga ggctggttag cagatagatt tgattataga aagataattt
    33481 taggttctgt atttcttaat ggagtagtat catttttatt tgcatttaat atgaactatc
    33541 catttgcagt tgctacttgg gtaggttgtg caatcacatc attagttatg aactatcctt
    33601 ctatggtaaa aatacttaga gttataggaa aagataatca aggtaaggtt tatggattta
    33661 atgaagctat ggttggagtt tctggagtag taatgggagc aatattctta tatatttata
    33721 catgctttgc aactcctaca ttgggtatga gatgggtaat gatatcttta ggaatattat
    33781 caatagcaat gtgtccagta ttatggtttg taattaaaga tgtagatatt aaagaagaaa
    33841 gagaagaagc aggtgaaaaa atgtctgctg gtgattttat aacagtacta aaatcaccaa
    33901 atacttggct agtaggaata agtatattct gtgtatattc attcacagtt acaatgtctt
    33961 attttacacc atatataaca tctgtacttg gaggttctgt agcattatct ggtgcattag
    34021 caataataag acagcatggt ctaaaattat ttggagcacc atttggagga tattgtgcag
    34081 acaaattaaa atctcctaca aaagtacttt taccaatata tgtatttggt atagcagtaa
    34141 tagttttatt cctagtatta cctgcatcaa ctccaatgac tatattcata gctttaacat
    34201 ttgtagtagg tatattaggt tacatgggaa aaggtatata ttatgcagta caagatgaag
    34261 taaaagttcc agttaaatat tcagcaacta ctataggtat agctgcagct ttaggatttt
    34321 caccagatgt attccaattt gcattaatag gacactggat agatacttat ggcaataaag
    34381 gttacacata tacatttata ttccaaatag caattctagt tattggaata ctatcttgtt
    34441 tatatatatt aaaggttaaa aagagaagag caagtaaatt agaagcttaa atattcaatt
    34501 tggtaattaa gtatatataa atacaatact tattttgatg aatgtgattg tataaataac
    34561 ataaaaaata tcaatagatt aagttttata ataatgttta gagaataaag tataaaaatc
    34621 aaaatgttat caagttttct aggttttaac ataaagttac ccctgaaggt gtaaaattga
    34681 gtggcaatta ttatctattt tcttacctac ataaacctat ttaatttaaa ttctaagaga
    34741 ttttcaaaat agatggtagt tgtactcaat tttaaacctc ataataaata cgaaaattta
    34801 aaatctaact agatactatt taattata
//
LOCUS       DAMJYC010000036        34721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJYC010000036 DAMJYC010000000
VERSION     DAMJYC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.037"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(117..773)
                     /locus_tag="PC472_001892"
     CDS             complement(117..773)
                     /locus_tag="PC472_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2727218.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILAEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(828..1457)
                     /locus_tag="PC472_001893"
     CDS             complement(828..1457)
                     /locus_tag="PC472_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2727219.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1588..2229)
                     /locus_tag="PC472_001894"
     CDS             complement(1588..2229)
                     /locus_tag="PC472_001894"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2727220.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2238..2909)
                     /locus_tag="PC472_001895"
     CDS             complement(2238..2909)
                     /locus_tag="PC472_001895"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2727221.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANIIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2914..3798)
                     /gene="rsgA"
                     /locus_tag="PC472_001896"
     CDS             complement(2914..3798)
                     /gene="rsgA"
                     /locus_tag="PC472_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2727222.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3896..4633)
                     /locus_tag="PC472_001897"
     CDS             complement(3896..4633)
                     /locus_tag="PC472_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2727223.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4741..6756)
                     /gene="pknB"
                     /locus_tag="PC472_001898"
     CDS             complement(4741..6756)
                     /gene="pknB"
                     /locus_tag="PC472_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2727224.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6773..7525)
                     /locus_tag="PC472_001899"
     CDS             complement(6773..7525)
                     /locus_tag="PC472_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2727225.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7545..8576)
                     /gene="rlmN"
                     /locus_tag="PC472_001900"
     CDS             complement(7545..8576)
                     /gene="rlmN"
                     /locus_tag="PC472_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2727226.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8584..9909)
                     /gene="rsmB"
                     /locus_tag="PC472_001901"
     CDS             complement(8584..9909)
                     /gene="rsmB"
                     /locus_tag="PC472_001901"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2727227.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFILDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQGVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9971..10672)
                     /locus_tag="PC472_001902"
     CDS             complement(9971..10672)
                     /locus_tag="PC472_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2727228.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10741..11493)
                     /locus_tag="PC472_001903"
     CDS             complement(10741..11493)
                     /locus_tag="PC472_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2727229.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(11503..12432)
                     /locus_tag="PC472_001904"
     CDS             complement(11503..12432)
                     /locus_tag="PC472_001904"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2727230.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12447..12887)
                     /gene="def"
                     /locus_tag="PC472_001905"
     CDS             complement(12447..12887)
                     /gene="def"
                     /locus_tag="PC472_001905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2727231.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEDLIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12905..15430)
                     /gene="priA"
                     /locus_tag="PC472_001906"
     CDS             complement(12905..15430)
                     /gene="priA"
                     /locus_tag="PC472_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2727232.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15669..16868)
                     /gene="coaBC"
                     /locus_tag="PC472_001907"
     CDS             complement(15669..16868)
                     /gene="coaBC"
                     /locus_tag="PC472_001907"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2727233.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16873..17139)
                     /locus_tag="PC472_001908"
     CDS             complement(16873..17139)
                     /locus_tag="PC472_001908"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2727234.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17142..17759)
                     /gene="gmk"
                     /locus_tag="PC472_001909"
     CDS             complement(17142..17759)
                     /gene="gmk"
                     /locus_tag="PC472_001909"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2727235.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17789..18670)
                     /locus_tag="PC472_001910"
     CDS             complement(17789..18670)
                     /locus_tag="PC472_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2727236.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18674..19504)
                     /locus_tag="PC472_001911"
     CDS             complement(18674..19504)
                     /locus_tag="PC472_001911"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2727237.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19791..20825)
                     /gene="ltaE"
                     /locus_tag="PC472_001912"
     CDS             complement(19791..20825)
                     /gene="ltaE"
                     /locus_tag="PC472_001912"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2727238.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21121..22896
                     /locus_tag="PC472_001913"
     CDS             21121..22896
                     /locus_tag="PC472_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2727239.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23111..24763)
                     /gene="abc-f"
                     /locus_tag="PC472_001914"
     CDS             complement(23111..24763)
                     /gene="abc-f"
                     /locus_tag="PC472_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2727240.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENRIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25062..26273)
                     /locus_tag="PC472_001915"
     CDS             complement(25062..26273)
                     /locus_tag="PC472_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2727241.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26557..27090)
                     /gene="pyrR"
                     /locus_tag="PC472_001916"
     CDS             complement(26557..27090)
                     /gene="pyrR"
                     /locus_tag="PC472_001916"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2727242.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27450..28397)
                     /locus_tag="PC472_001917"
     CDS             complement(27450..28397)
                     /locus_tag="PC472_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2727243.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALFINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28366..28812)
                     /gene="lspA"
                     /locus_tag="PC472_001918"
     CDS             complement(28366..28812)
                     /gene="lspA"
                     /locus_tag="PC472_001918"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2727244.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29122..29901)
                     /locus_tag="PC472_001919"
     CDS             complement(29122..29901)
                     /locus_tag="PC472_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2727245.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29956..30438)
                     /locus_tag="PC472_001920"
     CDS             complement(29956..30438)
                     /locus_tag="PC472_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2727246.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30704..32125)
                     /locus_tag="PC472_001921"
     CDS             complement(30704..32125)
                     /locus_tag="PC472_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2727247.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKQQNNHKLAS"
     gene            complement(32271..33041)
                     /locus_tag="PC472_001922"
     CDS             complement(32271..33041)
                     /locus_tag="PC472_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727248.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33046..34248)
                     /locus_tag="PC472_001923"
     CDS             complement(33046..34248)
                     /locus_tag="PC472_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2727249.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11058 a         6361 c         3482 g        13820 t
ORIGIN      
        1 tcaatgattt gaaatataaa attaatatca ataaaaccta ctttttaatt ttttaaatat
       61 ttatttttaa tttattttgt ttttaattta cttcgatttt aaaatgtcct tgacttttat
      121 tttataaatg tacatatttc ttctactggt tttttagatg gactcttcaa attgtcctct
      181 ggcactccaa gagctaacat tgcacctaaa aaatactctc ccttttcaaa accagtttct
      241 gcttctaaaa cagcttctat ctcatctgct gcatagtttt gacttgtcaa ccaacaagaa
      301 ccatatccaa gttcaattgc tgatagtgta aagttttcaa ttgcagcacc tagactctgc
      361 atacctggat ttcttataaa taatttatct atagtctcct taggtgcatc tataagctct
      421 aattcataat atcctgatgg gttatatacc tttgtaaaaa ctaatactaa aactggagct
      481 tttaaataaa atagtgtaaa gtttttaaca aattttctaa atctattagc cttatccact
      541 gatactttat ccatttctgc aagtatttct tgttgctttt tagttatcac atctgctatc
      601 ttttccatta aatcacggcg ttttataaca acaaaatgcc agttttgaat attcttgcct
      661 gatggtgctg cacctgctgc ttttatcatt tttaaaatat cttcatctga tacatcttga
      721 tttttaaact ttcttacact ttgtctttta aaaatagtat cttgtaattc catttttaaa
      781 tccccccata acttttttat tattttaata taatttttaa actcatctta ttttaatata
      841 tcatgttcta acaaatcttt aaatgagtct ataaaataat catatacatc tacattccca
      901 aacccatatt tggcatatat aaaaggtata cctacaaact tagcactatt tgcatcacct
      961 tgcgtatcac caacatagat tggatttatt aatttatttc tttcaattat aagtttatta
     1021 ttttctcctt tatgcaaacc agtattacca ggacattcaa aatctataaa atactgttct
     1081 agtttatgtg cttttaaaaa agattctata tatccagctt gacaattact tactataaat
     1141 aatttatatt ttttagatag taatttaaga gtatcttcta tatcatcaaa taatttagct
     1201 ccattttcag ataaatagtc acattctttc aaagaacatt cattcaataa attcattctt
     1261 tcttcttcac ttaaatttgg aaataaacga tttgctattt cctcaaaaga aagccccata
     1321 agacttctaa ataaatcttt tgtgacactt aattctattt ccttatgttt acttaaaaca
     1381 tcctgccaga ctttgcatac ttcttcagtt gaatcccaca atgttccatc taaatcaaaa
     1441 ataatactat ctaccacaaa aaatccccct tatgtcaaag tttattatat atttataatt
     1501 atatactaaa aagcagatac aatcataaat atattttact tgattgtatc tgctctacaa
     1561 tataaaacca attgtcaaaa tcaaatttta taaatttcta actacaagta cacagccttg
     1621 ctctacttta atcttacaag aattactcaa cataacattt gatattccaa taggtacaga
     1681 gtatttcata taataattat cgagagggta ttctaagcca gtaagtgtta cacccttagc
     1741 atcaccattt atagctatta cagaaatagt gtcccctggg tttccataca atgaaatttc
     1801 ctcattttca actatataca tctcttcttt atcagaaagt atcctagaat atataccatc
     1861 ttcttttaaa taatataata gctttatatt tgcaagagtg tggtcaatcc ttccccctaa
     1921 agcaccaaaa aaatcaatat gatttgcttt aagtgtctta gctaaaaaaa gacaaagctc
     1981 tgtatcagtc tcatcttttt tagatggaaa tttttcaaat ttaacacctt tatttttata
     2041 gaaatttatc ttttcttctt ctactgaatc taaatctcct aatatataat ctggcattat
     2101 ttccatttta taagtgtgat ttgcccctcc atctgcacat attatgcagt cataacattc
     2161 cttaattata atatctcttg taacttcgta atcttcaatt tcgccattta aaacaataca
     2221 gattttcatt attaaattta aactaattct tcaagagaag cattctttct aaataaatta
     2281 actgtttctt gaatgtcatc acttccaaat attgcagaac ctgctactat tatattagct
     2341 cctgcttgta caacttcggc tacgttttta gggcttatac caccatcaac ttctatatca
     2401 atgtttaatc cttgactatc tataatagct tttaactctt taatcttatc taaaacacaa
     2461 ggaataaatg attgtcctcc aaatccaggg ttaacagaca ttaataatac catatctaaa
     2521 tcaggtagta catgtttaat cgtatctact ggtgtagctg gattaagtac tacaccagct
     2581 tttattccat gtgattttat attttgtata gttctatgta gatgaacaca agcctcctgg
     2641 tgaacagtta ttatatcaca tcctgctttt acaaattctt caatatactg gtctggattt
     2701 tctatcataa gatgagcatc aaataccata tttatatttt ctttctttaa acttttgact
     2761 acaagtggtc ctaatgtaat gtttggtaca aagtgtccat ccattacatc gatatgaaga
     2821 tattcacatc ctgcactttc tacttttctt acatcttcta ataattttgc aaaatctgct
     2881 gataatattg atggtgctaa tttaatcatg tttctaatac ctcctactat tattttgtct
     2941 tatttcatgt agcagctgta tataactatc atatctttct ttagatattt ctccatttgt
     3001 aacagcttct tttatagcac aattaggttc attttcatga atacactttg aaccaaactt
     3061 acaatcatta aatttatcaa attctataaa ataatcttta agttcaactt cctctatatc
     3121 ttcaaggaca agggaactaa aacctggtgt atctgcaacc attccaccaa attctaattt
     3181 taaaagttca gcgtgacgag tagtatgttt tccccttttt attttatcac ttacaactcc
     3241 tgtttgaagt ttaaaatttt catctatttc atttaataag cttgattttc caactccaga
     3301 tggtcctgca aacactacta cattttcttt taattcttct tttactttat ctatatttaa
     3361 cttagtaata ttacttacag gaatgacttt atagccacta agttcatata tatttttaac
     3421 agtttctaat gtatcattat catctaaatc tgcttttgta aggattatta cagtttctaa
     3481 attttctttt tctgccaaaa ctataaatct atctaaaaga gataagtttg gtttaggatt
     3541 ttttattgcg aatactatta atgctttatc tacatttgca ataggaggtc ttataagttc
     3601 tgtatctcta ctgtcgattt cttctaagat accctttttt tcatcttcat caacaatgct
     3661 tattttgact ctatcaccaa ccaaaggtgt tattttctgc tttctaaata tccctctagc
     3721 cttacattca taaataccat tataagtatc tacataataa aatccactaa tcccctttat
     3781 aatttttcct tctagcatca aatgcctccc tttataaaat ttatataaac acatactgtt
     3841 tatgagtagt atatcgtgtt attctttcct atttttatat aataccacat tttttttaaa
     3901 taacaaatcc tccgtttgga ctaattacct gaccagtgat aaaatttgat ttgtctgatg
     3961 ccaagaaaat agcacaattt gctatatctt ctggagttcc tagcctcatt agaggggttt
     4021 cttccacaag agcgtctata tcttcctcat tatatccaga taacatgtca gtattaatga
     4081 cacctggagc tatgctattt actctaatat ttgatggtcc tacttcttta gctaatgcct
     4141 ttgtcatacc tattatgcct gcttttgtta tagaataatg cacttcacaa gaggcacctg
     4201 atatacccca gattgaagat atattaatta tatttccttt tttttctgat atcatgtatt
     4261 ttaaagcaac ttgtgaacaa taaaagcttc cttttaaatt aatattcatc atattatccc
     4321 aatcttcatc agttatatct gtaaacaact tatcttgact tacaccagca ttgtttacaa
     4381 gtacatccaa tcctccaaat tcttttatac aataatctac catatcctct acatcttctc
     4441 tatttgaaat attagcctta aaaagtttta cagaaaaatt tttttcattt aatatagtgt
     4501 acaactcttt agcttcattt tctgacttat taaaatttac taatacattg taaccttctt
     4561 ttgcaaaagc tttagacata gctttaccaa tccctctagc tcctcctgta attaaaactg
     4621 ttttcttctt cataaatact tttctcctta tattataacc aattcagtat tttatacaaa
     4681 acaaactgcc ctttaaagaa aatattttct ataaaaggac agctcttatt ttattaatat
     4741 ttattctgtt ttaccaatgt ttgcgccagt tcctgatgaa gttcctccat tatctcctga
     4801 gtttgagcct cctgaactat ctccattgtc tcctgagttt gaacctcctg aacttcctcc
     4861 attgtctcct gagtttgagc ctcctgaact tcctccatta tctcctgagt ttgaacctcc
     4921 tgagtttcca ccactatttc ctgagtttga acctcctgag ttacttccat tattgcctga
     4981 gtttccacca ctatttcctg aatttgagcc tcctgaattt ccaccactgt ttcctgagtt
     5041 tgaaccaggt tctgttggtg tattattctc tggtgttgtt ggttttttat ctggtactgt
     5101 tggagtctgt gtatttgatt tttctgaacc tttgcttaca tataggttta cctcatcacc
     5161 ttcctgagct cttgatgagc cagcacctgg actctgactt aaaactgtac cttctttata
     5221 tattgagctg tattcatact taacagttcc cacttttaag ttattttctt caataagttg
     5281 ttttgcatta ctcaaagtaa gtcccactac attaggaaca gatgcctgac ttgaaccttt
     5341 acttacgaca acatttatag tatcaccctt tttgacattt gtacttccac cttcaggtgt
     5401 ctgtgatatt atacaatttt catcaacttc actattatac tcttcacttt ttacactcag
     5461 gtataatcct tctttttcaa cagcactttg agcttcttct aatgtcatat ttacaagatt
     5521 aggaacagtc aaagatttat tgccaaaccc tccagcaaat aaaaacttat atgctaagaa
     5581 tacttgagca cacaatatca gtatcaaaac agccgcaatt acttttaatc ttcttctaga
     5641 ttttgggctt tcctgttttt tcttcttttt cttagttctc ttacttggag tactcttagg
     5701 tctctgattc tttttagctc tcattatctc ttcctcttct tcatcttcat catcatcttc
     5761 ttcataaaaa tcatcatagt aatcttcatc atcatccaaa tcagctactt caacaggctt
     5821 aaccactttt tcaggagctg gttttgcaag tgttggattt acaactttat taatttcctt
     5881 ttcatcaatt ttcttagttg caaaatcatc atattctttt ataaaatcta aatcaatatt
     5941 tttttctata tactctatat cttctatcaa ttcttcagca gtttgatatc tatctgcact
     6001 agatttttct gtaagctttt ttattgtagt tcttacactt tgaggaatcc taactttttc
     6061 ttctgatgta aagtctatat catcattaat gtgttgaagt gctatagaaa ttggactatc
     6121 tcccctaaat ggaacttttc ctatcaacat ttcatataaa actataccta gagaatacag
     6181 gtcagcatta ttagttacaa atttaccttt agcttgctct ggtgaaaaat aatgcactga
     6241 acctattata ctaccaatat tagtcattgt tgaatttgaa acagctttgg ctataccaaa
     6301 atcagccact tttactactc ttccttcatt tgatatgaga atattatgag gttttatatc
     6361 tctatgtata atcccctttt tgtgcgctgc actaagtgcc atagctattt gttttgttat
     6421 atctaatgca gtatattcat ctaatatacc ttcatcttgt ataatctctt ttagattctt
     6481 cccatctaca aactccatta caatgtagtg aacttttcca tcttcaccaa catcataaac
     6541 atttactata tttggatgtg aaagacttgc tactgcttct gcctctcttt taaactttgt
     6601 tagaaattca tcatcatcta caaattcagg tctaagtact ttaagtgcaa cagtcctgtt
     6661 taataatctg tctttagcct catatacaaa ggccattcct ccatcaccaa tcttcctgat
     6721 gatttcatat cgatttccta aaattgtatc tcccacaata tcaccgtcct aattatactt
     6781 ttatcactat aaccgaaaca ttatctttac cagaaacatc attggccata cttatcaatt
     6841 cttcacttat attactagtt ctctcatatt cataatctaa tattaaatct cttatatctt
     6901 catcatcaat aaacccagtt aatccatctg tcgcaagaag tattatatca ctttttttga
     6961 tttctttctt aaaaatatct acaacaacca tatcatctgt tccaattgct ctagttattc
     7021 tattcctctg tggatgtctt cttgcctctt cttctgtaat gacatgggcc atcataagtt
     7081 cttcaaccac ggaatggtct atagttattt tatcaaattt ttcatctgtt aacaaataac
     7141 atctgctatc tccaacattt gccacatata agacattatt atacactata gccgtaacta
     7201 atgtagttcc cattccttca aattctatat cttccataga ttttttgtgc acaattgagt
     7261 ttacattatt ataagcctgt tttaatatat catctatata gtctattttt acattatcat
     7321 gttgtaataa gttttccttt agaaaatcaa ttatattttc tactgctaat ttactggcga
     7381 cttcaccttt tttatgtcca cccattccat cagctatggc aaatattcct attggaccat
     7441 gttctgtatc tataacttct ccctcacaat agtcttcatt gttttttctt atttttccta
     7501 tatgggaggc acaactataa accatacgac ttcctcctta caaattactt gtattttctt
     7561 ctcaattgcc cacaagctcc actaatatca gaacccatag acatcctaac tgtggcaggt
     7621 atattgttct tctccaaact atccctaaat ttataaataa atgctttatc tggcttttca
     7681 tattctcttt cttcaacttt atttattgga attaaattta catgacatag catacctttt
     7741 aggagcttag ctaatgcctt tgcttctttt tctgagtcat ttaccccttt tataagagaa
     7801 tattcaaatg taacccttct atttgtcttt tttatataat atctacatgc attcaatatt
     7861 tcttcaatag aataagcatt tgctacaggc ataatttttc ttcgttcttc atcatatggt
     7921 gaatgaagtg ataaagctaa atttatagct atttctaagt ctgccaactc atacatttta
     7981 ggtactattc cacaagttga aagagttata tgtctatacc ctatattaag accatttttt
     8041 tcgttaatta tttttaaaaa ttgttttgta ttttcaaagt tatcaagtgg ctcaccactc
     8101 cccatcaata caagatttga taccctctta ccagtatctt cttgaatttt aataacttgg
     8161 tctaaaattt cccatggctc taagtttcta attaatccat ccattgtaga tgcacaaaaa
     8221 ttacatccca tcctacaacc tacttggtta gaaacacaga cagtaactct actatcataa
     8281 tccatcatca cagtttctat tatatttcca tcatctaata aaaacaaata tttttttgta
     8341 ttatctacct tagattctaa ctttaattct atatctatat gccctataca tgaaacctct
     8401 tctaatttat ttctcaaact ttttggtata ttgttcatat catcaaaagt ttttgcaccc
     8461 ttatatatcc aagagaatat ttgacttcct ctaaagggct tttcatctat agttttcata
     8521 aattctttaa gttcatcttc agtaaaattt tttaatacta tctttttctt ttccattctc
     8581 tcactacctt attctcttta attttgctat gaaaaaacca tctataccat gaatattagg
     8641 atatatcttt aaatatcctt tatcttgatt gtctaagtct acatttactg tatcaattgg
     8701 aacaaattca aagttgtgat tttcttcaac aaaagaagta actatattta tattctccac
     8761 accttgaatt gtacatgtac tatatactaa agttccacca actttgacat atttagatgc
     8821 attttctaaa attttctttt gtatagaagt gatgtccttt aactcttctt ctttcttata
     8881 cttaatttct ggtttacgtc ttattatacc aaaaccagag caaggaacat ctgctaaaac
     8941 ataatcaaat ttacttatac tattttcatc aatctcagaa gcatcaaatc tttctacaaa
     9001 cacattttta agccccaatc tatttacagt agagtttata agctttagtt tatgctcaaa
     9061 aatatctcta gctactactt gaccagtatt gttcattaaa gtagctaagt gagtagattt
     9121 ccctccagga gcactacata catccaaaat aaatgaacct tcttttggat taattacttt
     9181 ccctactatc atagaactta tatcttgaat tgtaaataaa ccagccttaa ataattcatt
     9241 tttttctata tttttcattt tttcgacttt aatagcttct tctaccatag gaacttttaa
     9301 acatacaata cccatttcac ttaatttttc aataagctct tctctactta ttttaagtgt
     9361 attcgttctt atatagatac taggcttttc attatttgct tccaacaaat cttcagcaaa
     9421 ttcttgtcca aatttatcta tccagttttt tataatccaa gagttatagg aatactttat
     9481 agataagtat tttacactat cttcctcagt tatgtccatt atagtctctt tagctcttat
     9541 ttcatttcta agaatagcat ttatgaaacc agaagatttt ttatcatatt tcttagataa
     9601 ttttacagtt tcattaactg ctgcataatc tgatatacta tctaaaaata aaatctgata
     9661 tgttcccatt cttaaaaaga ttttaacata agttgacatt ttttttacct ttatttttga
     9721 aagtttattg attatataat ctaaataata tttattttct attactccat aaattaactc
     9781 tgttgcaagt cctctatcca tatcacttat ttctaaattt ttaaaatgct tatttattgc
     9841 aatatttgaa taattgtcat ttttttctat atcacataat actttaaatc caacttctct
     9901 tgcatccata taaatttctc ccttttatta aacataaaaa agggttgttt ctaaacaacc
     9961 cactttactt ttagtcattt cttctttgag ctattagtaa caatctaagt aattgaagta
    10021 ttgaaaccaa tgctgctgca acatatgtta aagctgctgc tgaaagtact cttctacttt
    10081 gtcttgactc actttcatct ataataccta aattcccaag ttgaacgatt gctctacttg
    10141 aagcattaaa ttctacaggc aatgttatta tttggaataa cactgaagcc gaaaacaata
    10201 gtataccaat ttttaaaaat ggaccagcca taataaatcc taaaaatatt aaaaaccaag
    10261 atatacttga agcaaaattc actactggta ctaaactact tcttatttga agaggtgcat
    10321 aacctttagc atgttgtatg gcatgaccac actcatgtgc agctactgca acagaagtta
    10381 tagatgttcc ataatataca tcttctgaca accttacagc ctttcttctt gggtcataat
    10441 ggtcacttag atgccctcta accatttcta tctttacatc atacaaacca tttgaatcaa
    10501 gtactcttct tgctgtttgc tctccagtat atcctcgacg tgtatttact cttaaatact
    10561 tatttgttgt tgaactcact ttaaattgtg catatatagt aagtaatata gctggaatta
    10621 atacaaccat tgttgggtca aatccccaaa aaccgccata tacagggtac ataaataaca
    10681 ctctcctaaa atatattttt attatacatc ctcttaatat tcacttaact taaaaacagt
    10741 ttaattacaa actaatatat tttttcctgt taaaaagcct atagcttttt caatatccat
    10801 catgtctaca ttagtattta cacaaggtcc attaggtctt ttgttaaata cacctaaaac
    10861 aggaattttt ttaatatctt gtattccaga tgtcaaatct ctttcacatg caacagctac
    10921 aacagcttta ggtttagtgt ccattataat ttttctagct agggttcctc ctgtagctac
    10981 aaatattttt acattagttt tttcctttag cttaattaat tctcctatgt tgcatcttcc
    11041 acattcttta caattatcta tatcattagt aacttttaat ttacaactat tctcttgaat
    11101 acaatgaggt attaaaatta ttatatcctc tggattaaaa ttatatttat tactgtatat
    11161 atattcatta tttaacttta tatatattct tctaatatca ctttttgaca taccaaatga
    11221 tgatgcaatg aaagatataa taggaaataa tccactgaca atattaaaat ttattttcat
    11281 tatatttgag ttaacatact ttcctttaat tactctataa gtaactgctg ttgaacatag
    11341 tattataagt attagtgata ttattataat atttatcatc aaggagaata tttgagttaa
    11401 acttattata aaaaaattaa caactcctat caaaattcct aatattaaaa tcaaaatccc
    11461 aacacttgtt aaatacttct ttatatctgt catacttttc acctaaccca atactaaacc
    11521 agtttctata gtattccctt ttatatattc accaaccaac attctctttt tattcggcat
    11581 ctgaatttct tcaattaaaa gtacattatc tatagtactt acttttatcc catctttgct
    11641 tacatcaatt attgtaccta catcttttgt gcttttttca tctaacactt tagttttcca
    11701 aactttcatt atgacatcat tataggtagt ataagcactt ggccatggat ttactcctct
    11761 aactaaattg tgtatttctc tggcactttt tgaaaaatct atgttaccta atgatttatt
    11821 cattattggc gcataggaaa attcttcatg attttgtact tctcttggag catttccttc
    11881 ttcaattaat cttaatgttt cttttaaagt ttctgctcct atattcatca ttttatcatg
    11941 aagttctcct gctgttatat tttcatcaag atttacttct gtctttagta tcatatctcc
    12001 agtatctaat ccttcatcca tatacatagt tgtaacacca gtcttttctt caccatttat
    12061 tattacccaa tttataggtg ccgcacctct atattttgga agtaaagaaa catgaacatt
    12121 tatacatcca agtttaggaa tctctaatat ttcttttgga agtatctgtc caaaagctac
    12181 aactactatt acatctggat ttaaagattt aattttatct ataaattctt tatctctagc
    12241 ctttactggt tgatatacag gtatattatt ttcaattgcc agttctttta ctgggctcat
    12301 acctagtttt ttacctcttc cttttggttt atctggttga gttacaacac ccaatatttc
    12361 atacttctca tctattattt tttgtaaaca tggaactgct atatcaggag ttcccataaa
    12421 tactattttc atcaaatcaa ctcctactac ttttctattt tgtcaacaaa taaaattcca
    12481 tctaagtggt ctatctcatg acaaaatgct ctagctaaaa gctcttctcc ctctaactct
    12541 attgtttcac catttctatt taaagctctg acctttacat aatttggtct tctaacattt
    12601 cccgcttctc caacaacact taaacatcct tcctcatcta tttgctcccc agatgtttct
    12661 attatttcag gatttataag ttctataaga tcttctccaa tatctattac tactactctt
    12721 tttaatattc caacttgagg tgcagccagt ccaacaccat ctgcatcata catagtttct
    12781 gccatatcat ctaataattg tattatcttt tcatcaattt tttcaacctt ttttgatttt
    12841 tttcttaata ctggttctcc tatttgaact atttgtctta aagccattat aattcctcct
    12901 aaatttataa tacactgttt ggattaatat ctatagatac attgatattc ttatcaaata
    12961 tcaaatctcg ttttgttata catatatatt ttattatacc ctttaagaga ttaatttcaa
    13021 tattttcatc tttaaagagt atttgccatc tataattttg atttattttt gatattgaac
    13081 atggatttgg acctaatata aatcctaaat catttatacc ccttttttct aataaataaa
    13141 tcaatgatgc atgcatattt tttatatttt ttatcactaa tctttcatct tcaccactaa
    13201 caactacact taacatatta ttaaaaggag aatatccaaa tgcttttctt atttttatct
    13261 catcttcata aaaaccctca taatcatatt caatagcatg cttaatagca tagtgttcag
    13321 tgtcataagt ctgaagtact acttttccct ctttatcagc tctccctgct cttccagaga
    13381 cttgagttat caattgaaat gtagtttcaa aacttttaaa gtctggaaaa tttaatatca
    13441 tgtctgcaga caaaatacct actaaagtta cattctcaaa atccagccct ttacttaaca
    13501 tctgtgttcc aataagtaca tctgcttctt tatctttaaa tttatttaat atctcatcta
    13561 aagcaccttt ttttgaagtt gtatctttat ccattctcaa agtttttata tctggaaaaa
    13621 tatattttaa ttcctcttca attttttgag ttccaactcc aaatggctta acataagtac
    13681 ttttacattc aggacactct tttggaattt ctttctcata tccacaatag tggcatcttc
    13741 cagtattact tttcttatga taagttaaag atatatcaca attttcacat tgaaacacat
    13801 atccacactt tctacaagat acaaaatttg cataacctct tctgtttaaa aatagtatta
    13861 cttgattatt ttcttcaatc gcataacgta tttctttttg taatttcaaa ctaaatatac
    13921 ttctatttcc tttatctaac tcatctttca tgtctaccac ttcaatgttt ggaagaggct
    13981 tatcatttgc tctacttttt atatttataa gtttgtattc tccagatttt gccctataat
    14041 actcttcaat tgagggcgtt gctgaaccta aaataagagt tatattattt tttaatgcca
    14101 taaatctagc aacttctata gcattaaatt ttggattctt ctcagactta tatgatgact
    14161 catgacattc atcaattatt attaagccta aggaattaaa aggtgcaaat aaagctgacc
    14221 tagctccaat taacactctt actttacctg ctttgacagc tttgtaaaca tcatgttttt
    14281 ctccttcaga tagtttactg tggaacactc caactaaatc tccaaaccta ctctttagcc
    14341 tatctattgt ttgaggagtt aatgctatct caggtactaa gaatatactg tctaagcctt
    14401 gatttagagc aaattctatt atttccatgt aaacttcagt ttttccactt ccagttactc
    14461 catgaattaa gtatggtttt ttatcatcta caaacatatt cgactttatc tcatcaactg
    14521 cttcttgttg ctcattgttt aattttatac tttttagtac ggatttgtaa atactttttg
    14581 gctctctata ataatcctta aattctaggg ttattagctt tttcttactt agagaattta
    14641 tactttgttt tgaagcattt aataaatcca ataaatcatt tatctctaca ttttcattat
    14701 ttttaagaaa acttaatatc tctttttgtt tacttcccac atttatttta ttttgctcta
    14761 tataatcgtc aatcttatca ctttctaaac tcaaagatat ataacatact ttcttttcat
    14821 ttttgtgatt tttatattcc caacataatt ttataattcc atttttattc attgtgtaca
    14881 aagaactatt taagtttggt atatatttta acttatcaac cttaatttta cctttactat
    14941 caatcacttt ctctactatt tctctatttt tatcacttaa agttgatatt ttttccttca
    15001 attcaatatc atttaatcca cttaaacttt cacctaatag gactacttta taattattta
    15061 atttgtatcc ctttggatat atcaaattta tacattctat ataagtacac aaatatctat
    15121 ttttcatcca ataaaccaac tctaaatctt cttttctaaa tattggattt tcatctaata
    15181 tatcaataat ttcttttatt tttatttttt catctaagct atctgtaaat tgaaatacaa
    15241 aggcttctgt tggtttatta ccttttccaa atggtactaa tattctgtgc ccgattttta
    15301 ttacatcaga taaaaataca ggaatttgat atgtaaataa attatctgta tatatagtat
    15361 tacttctaac tattaccttt gcatattttt tcattataaa acctctactt aaatttaata
    15421 tattgttcat attttaatat actttacaag atattaaaat ttcagaattt ataaacaatt
    15481 tcaagttatt aatattaact ttattttaat aattaactat taattatatt ttatacttta
    15541 tgacaaaata aatgctgaaa actacaattt aactgacatt tgcgagtttt tactcgctaa
    15601 aattaaattg cagctttcag ccactttagc tgtgataaaa tacacaaata tcatatttaa
    15661 aaaaggtctt atttctcaag caacgcttta actttatcta aaataacatt ggctacttct
    15721 tcttttttca tcttaggata ctcagtaata tttccctcta catctattat ttttacaata
    15781 ttagtatcag ctctaaaacc agcaccttct ttagttaaat catttgccac aataaaatct
    15841 aaatttttct tttttatttt tcctttggca tgttctataa ggtcattagt ttcagctgca
    15901 aatccaatta gtatcttatc ttcttttatt tttcctattt cataagcaat gtcataattt
    15961 ctacttagtt ctattgataa atcatcattc ttctttttaa tttttttgtc agaataagtt
    16021 tttggtttat aatctgcaac tgctgcactt tgaattacta ttttattttc acttaaattt
    16081 gatactactg cttcatacaa atcttgtgct gactctattt gaacaaattt tttaagattt
    16141 tgtggaatag aaagatttgt aggacctgat attaatgtta catcagcacc tctagctata
    16201 gcttctcttg ctatagaata tcccatttta ccagaagacc tattagttaa atatcttact
    16261 gggtctatac tctctacagt tggaccagct gtaactatca tactttgacc ttttaaatct
    16321 tgctctttag taagattagt caaaacttcc tctactatta tatctggatt agctagttta
    16381 cctgttccaa tatctccaca tgcaagcctt ccactttccg gttcaataaa tttatatcca
    16441 aagctcttta aagtttgtat atttttttga acaattggat tttcatacat attagtattc
    16501 atagctggtg ctataagaac ctgtgctttt gttgccataa cagtagttgt caacatatcg
    16561 tcagctatac catttgcaat cttgcctata acatttgcac ttgctggcac tattaagaat
    16621 aaatctgctt tttttgctaa tgatatatgt tcaacttccc aagtttttgg ttcttcaaac
    16681 atatcttcta ctacataatt ttgactcagc gattgaaatg taagtggttt aacaaattct
    16741 gttgctgatt ttgtcattat aacatggata tttgcattta gtttttttaa cttacttaca
    16801 acatcacaag ccttatatgc agctataccg ccacttatgc caattacaac agttttatct
    16861 tttagcatga ccttactcct cttctgaaat ttgttggtgt tgctcagcat gatgtctagc
    16921 ttcttctatc tctatttcct cttctgtcaa taatctatat gttatttttc cacttgcaac
    16981 ttctttagtt gctacacata caggcttttc cttagtttta tttgaaacat atggttcttc
    17041 tccatcaatt aattttcttg ctctctttga aacagtccct actaaatagt atctgttgtc
    17101 tattttttct agtacctcat tgatagatgg ttttaacata attataattc ctccttaaat
    17161 ttctctatta taatattttt atatctgcta actttattct tttcagatga tattatacct
    17221 tctatttctt tagcagatgt ttttacatct tcattaaata taaaataatc ataatcctta
    17281 atcattttaa tttcttcata tgcacagcta aatctcttct ctatttcagc ttctgtttct
    17341 gtacctcttc caacaattct attttttaat tctttaagtg atggtggtag tacaaatata
    17401 aatactccct ctgggcaaac ttcttttatt tgttttgctc cttgcatttc aatctccagt
    17461 aatacatctt gtcctttttc taaacattcc attattgccg cttttggagt tccataaaaa
    17521 ttatcataaa tttgagcata ctccaaaaac tcaccttttt ctatcatctc ttcaaacttt
    17581 tccttactta tgaagaaata atttactcca tcaacttctc cagttctggg ttttcttgta
    17641 gtagctgata cagaaagttt tattgtatca ttttctttaa gtagttcttt gcaaatagta
    17701 ccttttcctg cacctgatgg tcctgataca actagcaata atcctttttt tacaaacatt
    17761 taagcattcc ccttctaata ttgtattctt actctatatt ttgtatttgt tctcttattt
    17821 tttccaattc gctttttact tctactacta aattagttat atttatatca gatgacttcg
    17881 aacctatagt gttagtttct ctattcatct cttgtattaa gaaatctatt tttctaccta
    17941 tagagtcgtc tttaaatata gtatttttaa gttgccctat atgactttta aatctaacta
    18001 tttcttctgt tatactgctt ttatctgcat atattgcaac ttcttgtgct aatctattct
    18061 catctattat acttgggtca tccagtaatt cacttattct caaattcaat ttttctctat
    18121 aatcttctac aacactactt gaatattttt caatctcttc tatatgattt tttaataaat
    18181 cacatctcat ggcaatatct tcagctaatt ttttaccttc ttcacttctc atttctctta
    18241 atttgattaa tgcatcttca actgcttgat tcaacattga ccacaataaa tcttcatctt
    18301 cttccttttc ttctattttc acaatatcag gaaactttgc aacattcata acgctaatat
    18361 catctataat atcaaattta ttttttattt cttttagtat gtctatatac tgagatgcta
    18421 atccctcatc aaaatttaag tttacatctt ctttgcctaa taagtcaaac tttatgtata
    18481 aatctactct tcctcttttt acataatttt ttactaaatt tctaacttta tcctctaaaa
    18541 atgaaatttt tcttggaaga cgtatgttta tatctgaata cttatgattt atcgttttac
    18601 actctactaa aaagtaataa ttatcatcct tgtattctcc tcttccaaaa ccagtcatac
    18661 ttatagccat taattaaacc tccaaaatac cttcacatat ttttactgct ggacctgtca
    18721 tataagagaa atctcctaca tctattttaa ctgttccacc ttgagacgtt acatttacac
    18781 tactttctac catattaagt aaattgcaca ctattgctga tgcagtcata ccagtaccac
    18841 aacctaaagt atatccacaa cctctttccc aagtataaac atgtatatta tacttatctt
    18901 ctacctttac aaaatttaca ttcgtttttt caggaaatat tttattattt tcaatctctt
    18961 ttccatattt acaaacatca tctacatcta attcatctac tataactact gtatgaggaa
    19021 cccccatcaa catagaagta actttaagct ccctatctaa aacctttata ctttcttgca
    19081 caaaacgttc tttatttgta agtacaggaa cgtccttagc tataaaactt ccttttccca
    19141 tattgactct tatagagttt atttctctat tggtaagatt tacttttata tcttttatac
    19201 catctaatgt atctactgta aaactttctt ttttaactat acaattatca taaacaaatt
    19261 tacaaaaaca tctaaggcca tttccacaca tagctgctct tgaaccatca gaattataat
    19321 ataccatttc tacatctgca acttttgaat ctttcacaac taacaatcca tccgctccta
    19381 cagaaaatct tctatggcat actttttttg ctaaagcagt ataatcagat ggatttatct
    19441 ggtcgaatct tccatctatt gctataaaat catttcctat tccatgtagt ttccaaaatt
    19501 tcataagtat cgctccttcg taaattaacg cttaaactat tataacataa aaaaattccc
    19561 ataaacttat aatttatggg aaaagttttg taatgacaac tataatttga ggatgtgagg
    19621 gagagttgcc attacaatta gggtaattat attatgtttt taagctagtt ctttctatgt
    19681 ttatatatta atatttctat atgaatttta tgtatgtttt atttaaactg tctatgaatt
    19741 ttttaattaa acatctggta actagctata atccttctag tagatatcta ttaacataca
    19801 cctattaatt ctttaagtgt ttttaaaaca tagtctaaat cttccttagt aatatctaca
    19861 tgagttacaa atctatattc accattttcc attttattaa tttttatatt tctttcaaaa
    19921 aattctttta ttaaaacatc ctcttttata atatcttcac tcatcttaaa aaataccata
    19981 cttatatctg gattaacatt atttacttca atgccatcaa ttttatttaa ttcacaagct
    20041 aaatattttg cattttcatg gtcttcattc aatcttaaag tcattttttc aatggcaatt
    20101 ataccagctg ctgcaactat acctacttgt ctcattcccc cacccataac tttttgcttt
    20161 cttctagctt ttgctataaa ttctttactt ccagcaagta tagaacccat tggtgcagct
    20221 aaaccttttg acacacagaa cataacacta tcacaacact ttgctatttc tttagcttct
    20281 acacccaaat ttaatgctgc gttaaataat cttgctccat ctaaatgtac tggtaagtta
    20341 tgtttctcag ctattttttt aatttctctt aaattatcaa gactcacaac tgttccacct
    20401 gaatatgcat tttctacaca tataagccct gtctgtggtt catgtatgtc atctcttcta
    20461 attgcctttt caacaagttc aggatttaga gctccacttt cgtcttcatc tatagttcta
    20521 agctgaactc cagctattac agcaggtgcc ccaacctcat gagtcacaat gtgataacct
    20581 ttaccaataa ttatttcttg ccctcttagg caatgagtaa atagagctaa ttgattccca
    20641 aaagttccac ttggaaggaa aatagcatct tcttttccaa ctttagctgc tgctaattct
    20701 tcaagtttat ttacagtagg gtcatctcta aagattgaat ctccaacagg agcctttgcc
    20761 atagcttctc tcatttcgtc agtaggcata gtaacagtat cacttcttaa atcgataaat
    20821 ctcatttttt caaccttctt tcatatttat atactataga accttaaaat tgattttttc
    20881 tgtaaagttc atatactttc atagcttata ctctacacaa taattatact atatttactt
    20941 tatatttaat ctactttaaa ttattaattt taaaaataaa gtttcctctc taataaatat
    21001 aaataatcat acaatttaaa tattaatctc tattgtttaa attcccccta gtcacaaagc
    21061 gtatattatg tgtataatta atttactgac tcattttggt caaagaaagg ggtgctataa
    21121 atggcattag atggattagt tatacattca atagtcgatg aattatcatc aaaacttaca
    21181 ggtggaaaaa tagataagat acatcaacct gaagatgatg aggtaatttt taatataaga
    21241 aataataaag aaaattttag attggtttta agtgcaagtg cctcaaatcc tagagtttat
    21301 cttacaagta attatcaaaa agaaaaccct ctaaaagcgc ctatgttttg tatgttactt
    21361 agaaaataca tacaaggtgg aaatatagta gaaatttctc aaataggttt tgaaagaata
    21421 ataaaaataa gtgttgaatc tttagatgaa ttaaaagaaa agacagttaa aaatattatg
    21481 attgaaatta tgggaagaca tagcaacata ataataactc atggtgaaga aaataaaatt
    21541 atagattcta taaaaagagt cccttttagc ataagtagag ttcgtcaagt tttacctggt
    21601 catgattatt cattaccacc agaacaaaat aaactcaacc ctcttgatga tatatctaag
    21661 gatttgttta ttaaaaacct agaggaattg gaaggaccta tctttaagtc tatatattct
    21721 aaatttctag gtataagccc tattatagct aaagaaattt gctacagagc aggtgtaaat
    21781 caaaatgcta tcataaagga tatatctgat gaacaatttg attctttaca caaagtattt
    21841 tgtaatttat tcaatgatat aaattctaac aaatactctc cttgcattat tatagataaa
    21901 aaggtggata aggtagttga tttcagttgt ataaacctta cattatttag cgatttatca
    21961 tatataaata aggatagtat gtctcgtatt ctggaggatt tctatagaac taaggatatt
    22021 aaagatagaa ttaatcaacg ttcatctgat ttaaagaaga gtatatctgt taagcttgat
    22081 agactttata ataaacttaa aaaacaagaa gaagaattat cagaatccga aaatgcagac
    22141 atttataaga taaaaggaga attgattact tcatatattt atatggtaga aaaaggtatg
    22201 gaaagtattg aagttgctaa cttctatgat gaaaattgta atgatgtaac aatagaatta
    22261 aataaaaact tgacaccatc tgaaaatgct caaaaatact ttaaaaagta taacaaaatg
    22321 aaacatgcta aagtcgagat atctcatcaa atttccttaa ataaggaaga aatagattac
    22381 ttagaaaaca taattttaag tattgaaaac tgtgaaaatt tagctgaatt acaagatata
    22441 aaagaagaac ttgctaaagt tggatacatc aagactcaaa agaaaaacag taaaaaggat
    22501 actatcccat ctacaaaacc tcatgaattt gtttcttctg atggatttaa aatattggtt
    22561 ggtaaaaaca ataagcaaaa tgactatctt acactaagac ttgctgataa tgatgactta
    22621 tggatgcata caaagaatat tcctggttct catgtaataa taaagtgtgc tggtaaggaa
    22681 gtccctgata atactgtatt tgaaggagct atgctagcag cattctttag taagtcaaaa
    22741 ctttcttctc aggtgcctgt agactatact aagagaaaaa atgtgaaaaa acctagtggt
    22801 tctaaaccag gtatggttat ttatgaaact aacagtacta tttatgttac tcctgaagaa
    22861 gaaacagtag ccaagcttaa ggttaaatct gaataaattc tgcactaaat acaaaataaa
    22921 tgttagacaa ctacacatta tcattctaaa taaaaatagt ggccttctta taaagtacaa
    22981 taataaggcc actttctaat tagcttactt ttaattttct aacaaaatac ttttctgttt
    23041 taataactat acttatattc ccaacaaata ttttcagtct tcttatatag atgtaattaa
    23101 aaagttaaca ctaaaagcta ttttttaatt tattgatttg ttttagtaat tcattatatt
    23161 ctgattcata caattctttt tttagattat ctttttctat agataacata gatataacag
    23221 ttgtaagtct attttctaat actagaagat tttctttttt gattcggttt tctctactag
    23281 gagttttttt atttctgcta attgtatatt cgtcataatt acaatcaaac tcccttattt
    23341 tagcatcttt tatctctata atatagttac aaatatgtga tacaaatact ctatcatgag
    23401 acactataag catagtttta tcagtattta tcagtgcctt ttccagtgct tccatagatt
    23461 ttatatctaa gtaatttgtt ggttcatcaa atactaaaaa attattatct tccaatatta
    23521 ttttacatag tgctattttt accttttctc caccactaag tacttttact tttttgtaga
    23581 catcatcacc tttaaaccca aataggctta agtttatcct tataaaagat tcatcaaatg
    23641 aactattaac tttagtattg tatagaacac tcttttcctc atctaataaa ctttgatttt
    23701 ggtcaaaata acctactttt accttgttat ttatttttat attgtcattt ttatcagcca
    23761 atatttcttt tatcagagta ctttttccac acccattatc acctaacaat gctatctttt
    23821 tacctctttt tatcttaaag gaaacattat ctaacaggag cttattttct aactttagag
    23881 tcatatcttt tacctctact agatttttgc taattatctc cataccatct tgcatcttaa
    23941 tgttcatttc tttagaaact ttaggtttat tctttacctc aagtttatca attctgcttt
    24001 ttatagcctt cacatttgag tccaactttt tcttattagt ctgaccaccc attttgtgca
    24061 acctagcttc tgaattaccc attctttttg gtgtttttct tatgccatca cttaaagctt
    24121 ttttatataa aagagctttt tcaagccttt ttttctcttt aacatattct tgatactcaa
    24181 aatcagctct ttttacttct tcatcttttt gcattaaata atcagtgtaa ttgcctttgt
    24241 aagcttttag ttttccatct tctagttcta atatagttgt acataactca tccagaaaac
    24301 gtctatcatg agatattaat aataatgctc cctcatgcct tttaaacata tcttccaaaa
    24361 ccccaatgct ttttttatct aagtttgaag ttggctcatc agctattatg atatttttct
    24421 tattactcag cgcatctgct atttttaact taaccttttc ccctcctgat aagtgttctt
    24481 caaatttatc tggagcattt aaaagactct tctccttact atgactacat gtttcaatat
    24541 tattttcact ctgacttata taagaatagc tttctgtaag gtaagtatat ccttcatcaa
    24601 tttctatttc tcccaatatt gcttttaata atgttgtttt tccttgacca tttgaaccaa
    24661 ctagacctat tttgtcattt tctaatattt caagtttatc tatatccaaa attaatttat
    24721 ctgcataata tttctttaaa ttctctactt ttactaacaa cataaaaaat ccctccttaa
    24781 cacacttaaa tataaggaga gattctataa ataggcataa caaataaacg tatagactat
    24841 aatctactct ccttaattta agcattgcaa ataaagccac caaaataggc ttttcaaaaa
    24901 ttacaatact ttaaattata aaaaatagat tatattctca ttgtcctagt taccttgccc
    24961 tttctttcat ttatttttaa tcattatatc aacattaatt acaaatgtct atagaaaatt
    25021 aactaatttt agttattcaa tatacaactt attctttaaa tttattcttc ttcaacttta
    25081 aagtcctgat tatttaaaat tctgtttaat acaattccaa caattgcagc taaactaaga
    25141 ccagttatct ttactgtact tgttataggg attccaatag aaataccttt attagataaa
    25201 tatgttgttc caagacctat taataatata actgctatga ttaatatatt tattttactt
    25261 tctacacaag aactgtccct tatagttttt aatcctacat aagttatcat cgaaaatagc
    25321 attatactaa ttccacccat aactgaacct ggtatacttt gaagaaatcc accaaacttt
    25381 cctacacatg caagcaatat agcaaatatt gctgtacgtc taagtattga agggtcatag
    25441 tttttagtta tagcaagtac tgctgtattt tctccataag ttgtatttgc tggtcctcct
    25501 aaacaccctg caactatagt cgcaaatcca tccccaagta atgttctatt taatccggga
    25561 ttttctataa agtttttacc tactacagca ccattagtag tcatatctcc tatatgttcc
    25621 ataaatacag ctaatacaac tggggatata atcacaattg ctcctaaact gaacttagga
    25681 actgtaaagt taggtattgc aaataaactt gcttctgtaa tagtagttat atctactaat
    25741 cttaatatca aacagattat atacccagaa aaaactgcta ttaatattcc taattgtttt
    25801 ataaatcctc taccaaactt attggttaaa attgctattg ctaaagttat aaatgctatt
    25861 acaaaattgg ctgaagccat actaaaagct gttggcaata aatttaatcc tataactact
    25921 atcatagctc ctgtaacttg tgcaggaaaa tataatttaa ttttatttac accaactact
    25981 tttactataa tcgacattat aatataaatt aatcctgcta ctattatacc accttgagca
    26041 tatgctaaat ctccaccata tgcctcttta gctgctagta taactggtat aaacgcaaaa
    26101 cttgaaccta aaaaggcagg tacttttcct tctgtacaaa aatgaaatat caatgtacca
    26161 acaccagcac agaaaattgc aactgacgga ttaaaacctg tcaaaatggg aactaatact
    26221 gtcgctccaa acatggctaa tagatgttgt aaagacatta tcgttttttt cataaaaacc
    26281 tccaaaatta ttatatataa ataaatactc tgtttaatta atatctttta attaattatc
    26341 attccctaga ttctattttt taacctctat atacttatta tataatttat cattatttta
    26401 cattttacga catcaaaaac tcaaataaag gagaataaaa actacattaa gactcacatc
    26461 cataaaatta taatatttat tttgtgcaat ctatgtaagc tttatcctcc tccctgacta
    26521 ttcaatgcta agcttttata taatacttaa aaataattat tctttgattg taactgaatc
    26581 ttctccatca atttctaata aactgacaga tataatttca tgtctagaag taggtacatt
    26641 ttttcctaca taatctgctc ttataggcaa ctctctatgc cctctgtcca ctagcactgc
    26701 taattgtatt gatttaggtc ttcctatatc tataatagca tctagtgaag ctctcacagt
    26761 tcttccagtg tataaaacat catcaactaa aactactgtc ttatcattta cattaaaatc
    26821 taaataagtt cctttaacta caggttcaac atgaattttt tccaaatcat ctctatacaa
    26881 agttatatca atatctcctg tatctacttt tgttccttct atttgttcta ttttcttaga
    26941 aattctattt gcaattggca cacctctagt ctttattcca actaaaacta gattttctac
    27001 acctttattt ctttcaatta tttcatgact tattctagta attgctcttg ctattgcctt
    27061 ttcatccatt aattgagctt tttctaccat atcataatta ttgtcaaaga tataatatat
    27121 ctatttgaca aatctactcc tttcttttaa ttttttataa atttattttt atttaaaatt
    27181 tcaatttaaa caacaaaaaa acttctcatc acaagacaag aagctcttat ataaaaaata
    27241 tacagtgcaa tttagcaatt gtcttacgat atctctgtat cgtttaaaga taatttttta
    27301 atctatcaaa tcaaattata tatttatatt tcaaattttt caagcttttt taaacaaaat
    27361 actttaatat aagatatttt atttaaattt cactaaattt atttacttat ataaaaacaa
    27421 aaaatgattt ataagtatta tacaaatggt tattttatct ttttaataac ttccttaaaa
    27481 tattcaggta attctgaatt aaattcgata tattccttag tagtaggatg tataaatccc
    27541 aacttcttag cgtgcaaagt ttgtcctttt aatttaaatt tagtttcttt tggaccatat
    27601 acataatcac ctaaaagtgg gtgatttata aacaaagcat gaactcttat ttgatgagtt
    27661 ctaccagttt caagcctagc tctcatatgt gtaaacttat caaatctttc tacaacttca
    27721 aagtgagtga ttgcattttt tccatctttt actacagcca tttttaatct atccttagga
    27781 tttctcccta taggtttatc aacagttatt ttatcttctt taaccacacc atagcatata
    27841 aattcatact ccctagttat agtatgttcc tttaactgtt ctgctagaaa attatgtgca
    27901 taattgttct tagctatcat aagaagacca gaagtatctt tgtctattct atgaactata
    27961 ccaggtctaa taactccatt tattgatgac aacttatctt tacaatgata taaaatcccg
    28021 tttactaacg tattttcata gtttccagga gcaggatgga caaccatatc ctgaggttta
    28081 tttattatta agacatcatc atcttcatat acaatatcta ttggaatatc ttgaggtaaa
    28141 acttctaata ctttaggttc tggtatctct ataacaatat tatcttcctc tttaaccaga
    28201 tatctagctt tttctacttt attattaacc tttacttttc catcttttat aattttttgt
    28261 atataacttc ttgacatatc acctaattgt tctgctagat aaatatcaag tctaacttct
    28321 tcttcctctt caagtactaa aaactctttt acttcatcca ttatatcacc tacttttact
    28381 ttcaaaaaat aaaacatata tacataaaaa cacagttcct acaactacaa atacatcagc
    28441 tatattgaat acatattccc atataattct aaagtcgaag taatctacta caaaacctaa
    28501 tcgtactcta tcaattagat ttcccaatgc accagatata attaatataa ttcccaacct
    28561 tccaaatata tgtactttcc ttgtattaag atagtatagt ccaaatactg ttgcaagtaa
    28621 tgcaacaatt ataaatatcc attgattatt ttgtaataat ccaaatgctg cacctctatt
    28681 ttcgacataa gttaaatgaa atacattatt tattattggt attgttgata catccaccaa
    28741 attattcaat acccatattt tagacagttg gtctaaacct atgagtagaa ttattattaa
    28801 tatatatagc aatgattttt ccccctataa attatttaaa aaccaatttt atttgaagca
    28861 cactattatt attatacaaa aaaaatgctc taaagagcaa ctttattaaa attgtatttt
    28921 cttaataaaa ctagaacaaa atttaaaata gtactacttt ttttatattg tagatttcta
    28981 catatttaaa atcatatcat tatgtataaa aaatttaaaa ataccatact aatcaatatt
    29041 ttttgtgtat tttatagatt aaagattaat gatgtctgta atttaaactt acaatcacca
    29101 ctaatcttca aacaaatatt tttacttttg cacttgctta tttttatcta aatgttgtat
    29161 aagttcagat actgttacga atttatatcc tctctctttt aatttaggaa ttactacttc
    29221 taaagcttgt atagtttgtg atttatcatt tcttttagtg ttataatcat gtaaaagtat
    29281 tatattacca ttttggacct ttgtaaggat agtatttata attgcatcta ctccagggtt
    29341 agaccaatct ctagggtcta agtttgacca aagaattata ttcatgcctt tagaaactat
    29401 aatatcacag acacttttac tcatggctct atatggaggt ctaaatatct ttggttctac
    29461 tcctattaca ctttttataa tattttgcgt atctgttatt tctttttcaa cactactata
    29521 actatttttt gcaacattta tatgtgtaaa tgtatgattc cctatttcat gcccctcatc
    29581 atattgtctt tttaataatt ctttgttata ttctactttt tctccaacta caaaaaaagt
    29641 tgcttttaca tcatttttct ttaatatatc aagtacttgt ggtgtaaaat cctcatctgg
    29701 accatcatca aaagtcaatg ctaaaatttt ttcatcctca tttccttttt ttattattac
    29761 atcttcataa tcgtctttta tattgtcatt taaactatat gcttcttttg aactatcttg
    29821 attatgtgta ttatgattat atgtatatac acctactata gataataaac atatacatat
    29881 accccatatt attttcttca tgtcattacc tcttttctaa ttcattctag caatatattt
    29941 tataatgtca gttttttaat ctctcaatat tccattttta tttaaatcag ttaaatttgt
    30001 taggtcttct gaataaaagt tagaatcttc tacatcaggg ttttgcattg catcacctaa
    30061 taaattattt tctttctttg cacaatcatt acataaactt gtatctggca atatatccaa
    30121 tctttctaaa tctatttgat tatgacataa atcacaaatc ccatatgttc cattctctat
    30181 tttagataat gctaagtcta tttgatacaa tcttccctct tcatgattta ttagactatt
    30241 ttgcatattt tgcatatata aatctgttcc catatctcct atatgattat cataactaga
    30301 taattctcct gatgaatact tttcacttgt atgtttagtt gtatctccaa ataaagtatt
    30361 atctttcata tcctctacta aattagttaa attttctctt tctttcaata atttatcttt
    30421 atatttttta gtatccattt ttaacacctc taattttaat atttacatca taaaaatagt
    30481 atgtggaaaa gtaaattaaa catacaataa aaataagatt tgattcgaaa aatagaatgt
    30541 ctcaaaatga aaattttagt tcatttaacc ctctttttta tctgaaatca aatatatttt
    30601 tatcctttca acaatgaaaa agagacttat ctctattttg agatagtctc ttctttgaac
    30661 ataatttaaa tataattttt taatttttat agttattatt ttactaactt gccaatttat
    30721 gattattttg ttgcttaata tgctttgctt ttttaaagaa taaaagacca agaattattg
    30781 ctacaataac acctattcca ttagatatat taactggtag tctaaatcct tcttttgctt
    30841 gcattatata actaaatgtt actacagtca taaaaagtgc tggtaatgta gttatataat
    30901 ggtttttatt tgctttgact aaatatgcag aaccagtcca caacattatc attgcaagtg
    30961 tttgatttgc ccaagagaaa tatctccaaa ttatattaaa atctataaat gtaagagcaa
    31021 caccaactgc gaataatggt aaagctatta aaaatctatt tttgactgca tcttgattat
    31081 aattcattgc atctgctatt gtcagcctag cacttctaaa tgctgtatct ccagaagtta
    31141 taggacatgc cacaactcca agtatagcta aagctcctcc aataggaccc attaatccaa
    31201 cagatactgt atttactact gttgctgctc caccatcatt aaatatcaca ttaagttgag
    31261 gtatgccatg gaaatatgac atggctgctg ctgcccaaac taaagctatt ataccttctg
    31321 caaccattga cccataaaat actggtcttg cctcactctc tttctcaaca catctcgcca
    31381 taagtggtga ttgagttgca tgaaaacctg atattgctcc acatgctatt gttataaata
    31441 agtatgggaa taaaggtgtt ccatttggat tcatattttg taaatttata tttggtatgt
    31501 tatacccctt tattattaat cctccagcta cacctactgc cataattaat aatgctgctc
    31561 caaataaagg atatatttta ccaatcaatt tatctattgg taaaacagta gccataatat
    31621 aataaagtat tatcactcca acccatattt gtgcgtttac acctgtcaat gtctttaata
    31681 atcctgctgg acctgttaca aatacaactc caaccaatac taaaagaata acactaaata
    31741 ttctcattgc tatttttgca ccatttccta gataaaggcc taccaactct gatgcagatg
    31801 tacctttgtt tttcattgat agatatcctg aagcaaaatc gtgtactcca ccagcaaaga
    31861 ttgttccaaa cacaatccat aaaaatgctg atggtccaaa taatgctcct tgtatagctc
    31921 caaatatcgg acctaaccct gctatattta gaaactgaat taaaaagatt ctcttccatt
    31981 tcattggcac ataatctact ccatccctac aagctatagc tggagtttga tttttgtcat
    32041 tggttccaaa aaccttttct acaaaagtac catagacaaa ataacctact ataagtacta
    32101 ctattgaacc taaaaagctt accataaatt catcccctta gtttttatat aattgcttta
    32161 taaattttta tgagtttaag aaaactattt ccagttcaaa tagtaatact tttttatgct
    32221 aatataaata ttaattacat taagtgcata tttaagtaca tgaaatgact ttatatatgc
    32281 attaacagcc taaattcttt tactttgctt ctacttacag ttacttcata atcactattt
    32341 tttaatttta atatgtatgt attattaaac catggtataa tttcttttac tttggttaag
    32401 tttacgatat aagacctatg tgttctaaaa aatgttttat catttataag attttcgact
    32461 tctgatatgc cttcttttaa tataaacttt tctttctcag tatatataaa tgtctgcctt
    32521 tcattagctt cacagtaata tatatcatca atatctatta caactaattt atcacctttc
    32581 caaaggctaa ttttatgtgt tatttcttct atctcctgat ttactgcttc cttcttatat
    32641 ttgtataaat tactatttac attagatagt tcaatatcac tcatctcact tttttctaat
    32701 ttattcagca tagaaataat tctctcttca gaatatggtt tcaatatata atcaaataca
    32761 tccaaagaaa aagcatctac tgcataactt tcatatgctg ttataaaaac aattttaggt
    32821 cttgatttaa actgatttaa tgtttttgca agtagcattc catcaagatg aggtatattt
    32881 atatctaaaa aaattacatc tattttattt tcctgaataa agtctaacac ctctatacca
    32941 ttggtaaatt cacttactac ctcgattcca cttttatttt caataaaata tctcaattcc
    33001 tttcttgctg gaaattcatc ttccactatt atagctctca tacttctact cctttacata
    33061 aaatactatc aaagtcccat tatctattct tcttatatta agccctcttc catacatcag
    33121 tttaagcctt aaatgtacat tataaagccc aatcttattt tctggcatat tatcttgata
    33181 tacattattt ataatttcct cacttatacc tataccatta tcctctattg atattctcac
    33241 agtattttct aatgaatacg gtaacttttt tatggatatt ttcactacac cagctctacc
    33301 actttctaaa ataccatgta ttattgcatt ttccactaat ggttgtataa tcaaacttgg
    33361 aatctttata tttacatctt ctatatcata agatacagtt agtaattctc caaacctagc
    33421 tttttctatc tctacaaaag atttaacttg ttcaatctct ttatttatat caattaagtt
    33481 atcactatat tcaagattat atcttagata agttgataaa tttattatta actctctagc
    33541 cttatctgga ctaaatctta taaatgaagc tattgtgttt agggaattaa acaaaaaatg
    33601 tggattaatt tgtctttgaa gtgcttttat ttctgcttta tttctcgctt ctatatcttt
    33661 tttatctttc tcaatcccct ctactataga tataacaaat cctattccaa tctgcccaac
    33721 tattaaaggt aaatatattc caccgattat ttgaattgct aaagaatgtg gatatgatat
    33781 taagtatata agtaatatag taaaactttc agaaatcatg cctaccaata ttccatacat
    33841 tactctatgt tgttttggta tcctcttata tagaaatcct gataatacac ctgctagtat
    33901 acttgataaa agacatggaa cagatgttat ccccccaata tccataaaat atctatgaat
    33961 ccctgaaaaa actcctgctg taatacctac catcggacca aataaaatgc cacctgatac
    34021 tatagatatt attctagtgt ttactattga ccccatataa tttataccac tataagttcc
    34081 aaatatggct aaaaaagtaa aaactaatgc tatacagacc aagtcattaa aactatattc
    34141 ttccttttga aatatctgct taaatatttt taactttgtt attagaagaa acaatactac
    34201 aataaggctt gtcttttcta ttaatgcgat taaaattgat gttttcataa tcccccttta
    34261 aaaaataaat ttttacacac tctaaattta ttatggttgt tataaattac atatttgaag
    34321 taatcctatc acaaaacttt ccacatttaa tcttttaatt aaataagaaa tagttgtact
    34381 tctattaaca actaatacta gctagcataa gcttgtaata acttgtagtt attattaact
    34441 ttacaattct agtatttaat tttatacttt agaaatctct tttcttagaa ataatacttt
    34501 cgaatatcat ataatacaat attatgtaga ttcttactca tacaacatat ttctttaaaa
    34561 attttatact attctttgtt caaatatctc atttgattta attgtgttaa acacacatta
    34621 tcatctctct gttatatatt tattatatag ccgtatatcg ataaagtaaa taaatcccat
    34681 atttaaaata atatgtttga caataaatta ttataatgta t
//
LOCUS       DAMJYC010000037        34253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJYC010000037 DAMJYC010000000
VERSION     DAMJYC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.038"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..511
                     /locus_tag="PC472_001924"
     CDS             <1..511
                     /locus_tag="PC472_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727250.1"
                     /translation="EYIYFKVSEKEEIQDIIEKIIIELYEPSVVNKVKIKLLVGLLLV
                     ELVKNSEDVENHAVDNYEKMMIIEILKYIDKDYKKGSLSEIASNLNQGDYKISKLVKK
                     HLGYTFKDLVQEKRLSKACELLVSTNLSIAEIIENVGYENLTYFYKIFKNKYGITPKE
                     YKKNIKNSQ"
     gene            718..2112
                     /gene="rhaB"
                     /locus_tag="PC472_001925"
     CDS             718..2112
                     /gene="rhaB"
                     /locus_tag="PC472_001925"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDF2727251.1"
                     /translation="MRYYLAIDIGASSGRHIISYINNGKLEIEEVYRFKNNLIKKDGH
                     FCWDISKLFSEIKNGIKKCIKINKIPVSVAIDTWAVDFVLLDEKDNILGNCVSYRDNR
                     TDGIMEEAFKIIDKKELYLKTGIQFQKFNTIYQLLAIKNQQPDLLEKAKSFLMIPDYF
                     NFLLTGKKVTEYTNATTTQLVNTTTNNWDEEIINKLGFDKMLFAKIKTPKTILGSLKK
                     ELVEELGCDLEVILPVTHDTGSAFMSTPVKNRSILLSSGTWSLLGVELERPKCKLEAL
                     DYNFTNEGGYEYRYRFLKNIMGLWMIQEARKCYDSKHSFTELVEHARLSEGFKSIVDV
                     NDDRFLNPENMVQEIIKYCIETNQTEPQTVGEVAYCIFNSLAKSYSKSINEIEEIIGE
                     KLETISVFGGGCQNELLNELIAKESGKKVLAGPVEATALGNIVCQLIAKNELNDLEQA
                     RDIIRNSFDIIQYN"
     gene            2133..3389
                     /gene="rhaA"
                     /locus_tag="PC472_001926"
     CDS             2133..3389
                     /gene="rhaA"
                     /locus_tag="PC472_001926"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDF2727252.1"
                     /translation="MELKQRYEVAKKEYEKWGINVEEVLRVLSKVKISIHCWQGDDVT
                     GFEGNQQRLSGGIASTGNYPGKARNPQELRRDLDKALSLIPGKHKVNLHAIYAETGGS
                     FVDRDELKPEYFENWVKWAKERGLGLDFNPTIFSHPKSSDGLTLSHPDKKIRDFWIKH
                     CIASRKIGEYFGKELGQTCLTNIWIPDGYKDIPSDRLGPRRRLKESLEEILKVEINRE
                     YNLDCVESKVFGLGVESYTVGSNEFYLNFASKNNIISLLDTGHYHPTELVSDKLSSML
                     LFSDKVALHVSRPVRWDSDHVVILDDELKEIAKEIVRNDALDKVIIGLDFFDASINRI
                     AAWVIGTRNMIKALLSAMLMPHEELRKLQDEGDFTKRLAIMEEFKTYPMGDIWNYYCK
                     INNVAVKEDWIEVVKEYELTELLNRK"
     gene            3400..4215
                     /gene="rhaD"
                     /locus_tag="PC472_001927"
     CDS             3400..4215
                     /gene="rhaD"
                     /locus_tag="PC472_001927"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDF2727253.1"
                     /translation="MSIMEMNFIKDFMKITYDAWLKGWHERNGGNITYRLTPEEISGI
                     KEFITEEIEYIPIGVTVENLANEYFLVTGSGKFMRNISINTVQNVGIVKIDEKGENYA
                     IVWGLDKAKPTSEFPSHLMSHSIKKDKTNGLHRVIMHSHTTNLIALTFILPLEGKVFT
                     RELWEMATECPVVFPEGIGIVPWMVPGGIEIAKATQNLMEKHNIVIWAHHGTFCSGET
                     LDLTFGLMDTVEKSAEILVKVISMGGKKQTITGDNFRDLSKNFKVSISEDYLY"
     gene            4492..5958
                     /locus_tag="PC472_001928"
     CDS             4492..5958
                     /locus_tag="PC472_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2727254.1"
                     /translation="MKREVTIQKQAISIVLFMAVLFCGLLLPMINNKSEYSLPIESIM
                     FISASIISLFIVVTTNKTWEEVFESGLDGVKGALPGLIILLVIGPLIAGFIMSGLMPM
                     LVYYGVKLINPRFVYATALILSALFSSFTGTSWGSAATIGVVMMGVGNAIGANNAIIA
                     GAIVGGAYFGDKMSPLSDTTNIAAIASKVNLYDHVASMLWTTGPATIIALVIYIACGF
                     IFPATNTNLNSPEITRLLADLSGMFNFNIILFIPLLIVLVGSAMKKPAIPVMTIAAFL
                     SLAFAVIFQGFSLADVLIGLNSGFSLDMVTWHEYAKPIEGQAYILGFFQKGGFWELGK
                     LLPISTSILFVVGVLGSIDAMPATVNVIFGWVKSRTAIICSSLLTGIAMIAMTANGIA
                     CSFVTAGIFGDKYDENNIDRRVLSRTTEDTGTLLEVLFPWTPAAIFFTQTLGVNVGEY
                     ALWSIVNWGTPIIAVILAVTGIGTYKNSKDKKQSNKVS"
     gene            6058..7248
                     /locus_tag="PC472_001929"
     CDS             6058..7248
                     /locus_tag="PC472_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDF2727255.1"
                     /translation="MDKNKYKIETLMNHVGENLDGDYGAVNPPIYQSSIFARSTYEDI
                     EAYFEGSKDGYLYSRTKNPTNDILEAKLSALANAEASITFGSGMGAISSAILHFVKNG
                     DHIIASNNLYNPTMNFITKELKEKGNIENTIVEGDRIEDFENAIKQNTTLIYLECPST
                     AVFKMQDIKAIVALAKTHNIKTVIDNTWATPLYQRCIEMGIDLEVHSLSKYLNGHSDV
                     VGGCIIGSKKDIEEIRGSELALYGAKLAPFEAFLVIRGLRTLHARLSVHTKNAKEVVE
                     FLDNHSAVTKVYHPLSKYFNQKKLAKKQLLNYTALLSFEIDAKDVNDVKKFVNKLDLF
                     KIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLINDLNDA
                     LSIL"
     gene            7441..9399
                     /locus_tag="PC472_001930"
     CDS             7441..9399
                     /locus_tag="PC472_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HDF2727256.1"
                     /translation="MKRAYYSSSVNEFIVKDIFTIFGEITCNDQFAADDLQKNTWKKE
                     IGILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPKHAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQKDQRIRMMKENILETYCCNEFNIGKYI
                     KKICTTYNRSSFNSDTWINSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKTHNRKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGIRKSEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFESLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSENVSTFVKAVLDVDKVAAKELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQNKIDATNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNNHFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFIIFVPI
                     GDESDITMQPNFYNGVYEYLKEIGIEEL"
     gene            9942..11315
                     /locus_tag="PC472_001931"
     CDS             9942..11315
                     /locus_tag="PC472_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2727257.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            11570..12637
                     /locus_tag="PC472_001932"
     CDS             11570..12637
                     /locus_tag="PC472_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2727258.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            12781..13089
                     /locus_tag="PC472_001933"
     CDS             12781..13089
                     /locus_tag="PC472_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2727259.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            13082..14512
                     /locus_tag="PC472_001934"
     CDS             13082..14512
                     /locus_tag="PC472_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2727260.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            14590..14964
                     /locus_tag="PC472_001935"
     CDS             14590..14964
                     /locus_tag="PC472_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2727261.1"
                     /translation="MKKREDDFEVRRKHLQELSEDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            14958..17162
                     /locus_tag="PC472_001936"
     CDS             14958..17162
                     /locus_tag="PC472_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2727262.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            17162..18571
                     /locus_tag="PC472_001937"
     CDS             17162..18571
                     /locus_tag="PC472_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2727263.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            18556..19620
                     /locus_tag="PC472_001938"
     CDS             18556..19620
                     /locus_tag="PC472_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2727264.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVAKKIIV"
     gene            19650..21071
                     /gene="nhaC"
                     /locus_tag="PC472_001939"
     CDS             19650..21071
                     /gene="nhaC"
                     /locus_tag="PC472_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2727265.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VIFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            21500..21964
                     /locus_tag="PC472_001940"
     CDS             21500..21964
                     /locus_tag="PC472_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2727266.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            22198..22560
                     /locus_tag="PC472_001941"
     CDS             22198..22560
                     /locus_tag="PC472_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2727267.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            23155..23991
                     /locus_tag="PC472_001942"
     CDS             23155..23991
                     /locus_tag="PC472_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727268.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            24075..26189
                     /locus_tag="PC472_001943"
     CDS             24075..26189
                     /locus_tag="PC472_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2727269.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLENVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMVEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            26730..27683
                     /gene="blaCDD"
                     /locus_tag="PC472_001944"
     CDS             26730..27683
                     /gene="blaCDD"
                     /locus_tag="PC472_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2727270.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            28027..28710
                     /locus_tag="PC472_001945"
     CDS             28027..28710
                     /locus_tag="PC472_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727271.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            28707..31367
                     /locus_tag="PC472_001946"
     CDS             28707..31367
                     /locus_tag="PC472_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727272.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQVSVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(31548..32729)
                     /locus_tag="PC472_001947"
     CDS             complement(31548..32729)
                     /locus_tag="PC472_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727273.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(32729..33391)
                     /locus_tag="PC472_001948"
     CDS             complement(32729..33391)
                     /locus_tag="PC472_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727274.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(33523..34161)
                     /locus_tag="PC472_001949"
     CDS             complement(33523..34161)
                     /locus_tag="PC472_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2727275.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT        13423 a         3881 c         6102 g        10847 t
ORIGIN      
        1 agaatatata tattttaaag tatctgagaa agaagagatt caagatataa tagaaaaaat
       61 tataatagaa ttgtatgaac caagtgtggt taataaggta aagattaaat tattagtagg
      121 attattatta gtagaattag ttaaaaattc tgaagatgta gaaaatcatg cagtagataa
      181 ttatgaaaag atgatgatta tagaaatact aaaatatatt gataaggact acaagaaagg
      241 tagtttatct gaaattgcaa gtaatttgaa tcaaggtgat tataaaataa gtaaactagt
      301 aaaaaaacat cttggatata cttttaaaga tttagtgcaa gaaaaaaggt taagtaaagc
      361 atgtgaatta ttagtatcta ctaatttatc aatagctgaa attatagaaa atgtaggtta
      421 tgaaaattta acttattttt ataaaatatt taaaaataag tatggtatta caccaaaaga
      481 atacaaaaaa aatataaaaa atagtcaata gtaaaattag aatctataaa atagattctt
      541 ttttattatc aaataaacca ttttaatagc tctgaagata ttatattttg aaaattatta
      601 ttgagaataa agttatattg caaattaggt taataaatat gacaaattta gatattaaat
      661 gaatatacta acatcttgta taattcaatt aataaataaa tctaaggagg aaaagtcttg
      721 agatactatt tagcaattga tataggggca tctagtggaa gacatattat ctcttatata
      781 aataatggaa aattggaaat agaagaagta tatagattta agaataattt gattaaaaaa
      841 gatggtcatt tttgttggga tatatcaaag ctattttctg aaataaaaaa tgggataaaa
      901 aaatgtataa aaataaataa aattccagta agtgtggcta tagacacatg ggctgtagat
      961 tttgtgttac ttgatgaaaa agacaatata ttaggtaact gtgtatcata cagagataat
     1021 agaactgatg gaattatgga agaagccttt aaaataattg ataaaaagga actatattta
     1081 aaaacgggaa tacaatttca aaagtttaat actatatatc aattattagc aataaaaaat
     1141 caacagccag atttattaga aaaggcaaaa agctttctaa tgattcctga ttattttaac
     1201 tttttattaa ctgggaaaaa agttacagaa tatacaaatg caacaactac acaattagta
     1261 aatacaacaa caaataattg ggatgaagaa ataataaata aacttggatt tgacaagatg
     1321 ttatttgcta aaataaaaac tcccaaaaca atactaggta gtttaaagaa agaattagta
     1381 gaagagttag gatgtgattt agaagtcatt ttaccagtta ctcatgatac aggttcagct
     1441 ttcatgtcta ctcctgtaaa aaatagaagc attttattga gttcaggaac atggtcttta
     1501 ttaggagtag agttagaaag accaaagtgc aaattagagg ctttagatta taattttact
     1561 aatgaaggtg gatatgaata tagatataga tttttaaaaa atataatggg tttatggatg
     1621 atacaagaag ctagaaagtg ttatgacagt aagcactctt ttacagaact agtagagcat
     1681 gcaagactat cagaaggatt taaatcaata gtagatgtaa atgatgatag atttttaaat
     1741 cctgaaaata tggtacaaga aataataaaa tactgtatag aaactaatca aacagaacca
     1801 caaacagttg gagaagtagc atattgtatt tttaacagtt tagcaaaaag ttatagtaaa
     1861 tctataaatg aaatagaaga aatcatagga gaaaaattag aaactataag tgtatttggt
     1921 ggaggatgtc aaaatgaact gttaaatgag cttatagcaa aagaaagtgg taaaaaggta
     1981 ttagcaggac ctgttgaagc tacagcatta ggtaatatag tatgtcaatt aattgctaag
     2041 aatgagctaa atgatttaga acaggcgaga gatataataa gaaattcatt tgatataatt
     2101 caatataatt aatctgaaaa aggagataag tgatggaatt aaagcaaagg tatgaagtag
     2161 ctaaaaaaga atatgaaaaa tggggaataa atgtagagga agtattaaga gttttatcta
     2221 aagttaaaat atctattcat tgttggcaag gtgatgatgt aacagggttt gaaggaaatc
     2281 aacaaagact atcaggagga attgcatcta caggaaatta tcctggaaag gctagaaatc
     2341 ctcaggagtt aagacgagat ttggataagg ctttaagttt aataccaggg aaacataaag
     2401 ttaacttaca tgctatatat gcagaaactg gtggaagctt tgtggataga gatgagctaa
     2461 aaccagagta ttttgaaaat tgggtaaaat gggctaaaga aagaggattg ggattagatt
     2521 ttaacccaac aatattctct catccaaaat caagtgatgg gttaacattg tcacacccag
     2581 ataaaaaaat acgtgatttt tggataaagc attgcatagc ttctagaaaa ataggcgaat
     2641 actttggaaa ggagcttggt cagacttgtt taacaaacat atggatacca gatggataca
     2701 aagatatacc aagtgataga ttaggtccta gaagacgctt aaaggaatca ctagaagaaa
     2761 tattaaaggt agagataaac agagaatata atttagattg tgtcgaatca aaagtttttg
     2821 gattaggtgt agaatcttat acagttgggt caaatgagtt ttacttaaat tttgctagta
     2881 aaaacaacat aatttcatta ttagatacag ggcactatca tcctacagaa cttgtttcag
     2941 ataaattatc atcaatgcta ttattttcag ataaagttgc acttcatgta agtcgtccag
     3001 tgcgttggga tagtgaccat gtagttatat tagatgatga actaaaagaa atagccaagg
     3061 aaatagtaag aaatgatgct ttggataaag taataatagg actagacttc tttgatgcaa
     3121 gtataaatcg tatagcagca tgggtaattg gaactagaaa tatgataaaa gcattgttat
     3181 cagctatgtt aatgcctcat gaagaattaa gaaaattaca agacgaaggc gattttacaa
     3241 aaagattggc tataatggaa gagttcaaaa catatccaat gggagacata tggaactatt
     3301 attgtaaaat taataatgtt gctgttaaag aagattggat agaagtagta aaagagtacg
     3361 aattaacaga attattaaat agaaagtagg taagggtata tgtctataat ggaaatgaac
     3421 tttatcaaag attttatgaa aataacttat gatgcttggt taaagggatg gcatgagcgt
     3481 aatgggggga atataacgta tagattaaca cctgaagaaa tatctggaat aaaagaattt
     3541 ataacagaag aaatagaata tattccaatt ggtgtaacag tcgaaaattt agctaatgaa
     3601 tatttcttag ttacaggaag tggcaaattc atgagaaata tttctataaa tactgttcaa
     3661 aatgtaggaa ttgttaaaat agatgaaaaa ggtgaaaatt atgcaatagt ttggggatta
     3721 gataaggcta aaccaacaag tgaatttcca tctcacttaa tgagtcattc tataaaaaaa
     3781 gacaaaacaa atggattaca tagagtaata atgcattcac atacaactaa tctcatagca
     3841 ttaactttta tactgcctct tgagggtaaa gtatttacaa gagaattatg ggagatggct
     3901 acagaatgtc cagttgtatt tccagaagga attggaatcg taccttggat ggtgccaggt
     3961 ggtatagaga tagcaaaagc tacacaaaat ctaatggaaa aacataacat agtaatctgg
     4021 gctcatcatg gaacgttctg ctcaggagaa actttggact taacatttgg tcttatggat
     4081 acagtagaaa aatcggctga gatattagta aaagtaatat ctatgggagg gaaaaaacaa
     4141 acaataacag gagataactt tagagactta tcaaaaaatt ttaaggtttc aatatcagag
     4201 gattatttgt actagataag ataaaagata aacaaagcta tgataattta ttatgtacaa
     4261 ttaataaaac actatgagag aggaggtgat taaaagatat agaaagtaaa ttcacttaag
     4321 aaaatagttt aagaataaac tggagtttat aataaaatat tcaacaatat atttatatat
     4381 ataaatatat tgttgaatat aaatataaga tagtaaattt tattgttaga taagggaaaa
     4441 cgttattaaa aattgaatta atgagtaaat aaaagtggga ggaaataata aatgaagaga
     4501 gaagttacaa ttcaaaagca ggctatatct atagttttgt ttatggcagt tttattttgt
     4561 gggctattac ttccaatgat taacaataag tcggaatata gtttgccaat agagtcaata
     4621 atgtttattt cagcatcaat aatatcgcta tttatagtgg ttacaacaaa taaaacgtgg
     4681 gaagaagtat ttgaaagtgg attagatggt gtaaaaggag ctttaccagg attaataata
     4741 ttattagtaa taggtccact tattgcagga tttattatga gtggattaat gcctatgtta
     4801 gtatattatg gagttaaatt gataaatcca agatttgtat atgctacagc attaatattg
     4861 tcagcattgt tttcttcatt tacaggaact tcctggggat cagcagcaac tataggagtt
     4921 gtaatgatgg gggtaggtaa tgcaatagga gcaaacaatg ctattatagc aggtgctata
     4981 gtaggtggag cttactttgg tgataaaatg tcacccctat cagatacaac aaatatagct
     5041 gcaatagcgt caaaagttaa tttatatgac catgttgcat ctatgttatg gacaacaggt
     5101 ccagcaacta taatagcttt agttatatat attgcttgtg ggtttatatt cccagcaaca
     5161 aatacaaatt taaatagtcc agaaataact agattattgg cagatttatc aggaatgttc
     5221 aatttcaata taattttatt tatacctctt ttaatagtat tagtaggttc agcaatgaaa
     5281 aaacctgcaa taccagtaat gacaatagca gcatttttat cacttgcatt tgcagtaatc
     5341 tttcaaggtt tttctttggc agatgttctt ataggactta acagtggatt ctcactagat
     5401 atggtaactt ggcatgaata tgcaaaacct atagaaggac aagcttatat attaggtttt
     5461 ttccaaaaag gtggcttctg ggaattaggt aaattattgc caatatcaac atctatatta
     5521 tttgttgttg gagtacttgg aagtattgat gctatgccag caacggtaaa tgtgatattt
     5581 gggtgggtta agtctagaac ggctataata tgttcttcat tattaacagg aatagcaatg
     5641 atagcaatga ctgcaaatgg tatagcatgt agttttgtaa cagcaggtat atttggagat
     5701 aaatatgatg agaataatat agatcgtagg gtactatcaa gaactacaga agatacaggt
     5761 acattgttag aagttttatt tccttggaca ccagcagcaa tatttttcac tcagacacta
     5821 ggagttaatg ttggagaata cgcactttgg tctatagtga actggggaac tccaattata
     5881 gcagtaatct tagcagttac agggataggt acatataaaa attcaaagga taaaaaacaa
     5941 tctaataaag tatcataatg aatattttaa ctttattacc taacatagct tataaaaaat
     6001 aagctatgtt aaatgtattt ttcatatata aaaatatttc ataaggagat ttagaacatg
     6061 gataaaaata aatataagat agagacatta atgaatcatg taggagaaaa tttggatggc
     6121 gattatggag ctgtaaaccc acctatatat caatcatcaa tattcgcaag aagtacatat
     6181 gaagatatag aagcatattt tgaaggttca aaagatggat atctttattc aagaactaaa
     6241 aatcctacta atgatatttt agaagcaaag ttatctgcat tagctaatgc tgaagcaagt
     6301 ataacctttg gttcaggaat gggtgctata tcaagtgcta tacttcattt tgtaaaaaat
     6361 ggtgaccata taatagcttc aaataattta tacaatccta caatgaactt tataacgaag
     6421 gaattgaagg agaaaggaaa tatagaaaat acaatagttg aaggtgatag aatagaagat
     6481 tttgaaaatg ctataaaaca aaatacaact ttaatatacc tagagtgtcc atcaacagca
     6541 gtatttaaaa tgcaagatat aaaggcaatt gtagcactag caaaaacaca caatataaaa
     6601 acagtaatag ataatacttg ggctacacca ttatatcaaa gatgtattga gatgggaatt
     6661 gatttagaag ttcattcttt atctaaatat ttaaatggac attcagatgt ggtaggtgga
     6721 tgtattattg gaagtaaaaa agatatagaa gaaataagag ggagtgaatt agctttatat
     6781 ggtgcaaaac tagcaccatt tgaagcattt ttagttataa gaggtttgag aactttacac
     6841 gctagattat cagtacatac caaaaatgct aaggaagtag ttgaattttt agataatcat
     6901 tcagcagtta ctaaggtata tcatccatta tctaaatatt ttaatcaaaa aaaactagca
     6961 aagaaacagt tgcttaatta tactgcgtta ttaagttttg aaatagacgc aaaggatgta
     7021 aatgatgtta aaaaatttgt aaataaatta gatttattta aaataggagt gagttgggga
     7081 ggccatgaaa gtttagtatt tgctccaaat atagctatat taaatgaaat gactttagaa
     7141 caagccaaag cattaaatgt gtcaccatac acaattagaa taagtttagg tcttgaaaat
     7201 agtgaagatt taatcaatga tttaaatgat gcgctatcca ttttataata taataagcat
     7261 atatggaaaa aacatattaa ctaaatagtt tataatgtac ttagcaaaga aaaattatta
     7321 tcaaagtacc atacataaac atatctatgt agtgtattta taaagtatag gagtcaagtt
     7381 ttagactgta ggatagatgt aggctataaa atgaatatta gatataaagg gggaagatgt
     7441 atgaaaagag catattattc aagtagtgta aatgaattta tagtcaaaga tatttttact
     7501 atatttggtg agataacgtg taatgatcaa tttgcggctg atgacttaca aaagaataca
     7561 tggaaaaagg aaattggaat attaaaaaga gagctctcaa ctttcaaaga tggacactta
     7621 ttatttgagt atacaatacc acgaataggc agtagaattg acaatgtgtt aatttacaag
     7681 ggcattgttt ttctccttga atttaaagtt gatgaagaca cttatcctaa gcatgcaatt
     7741 gaacaagtta cagactatgc ccttgacctt aattgttttc acaaagaaag tcatgataaa
     7801 cttttagtac caatacttat ttgtacaaaa gcccctcaaa aagaccaaag aataagaatg
     7861 atgaaggaaa atattttaga aacttattgt tgtaatgagt ttaatatagg aaaatacata
     7921 aagaagattt gtactacata taatagaagt tcatttaatt cagacacttg gataaactcg
     7981 ttatacatgc caactccaac aattattgaa gctgcacaag ctttatatat gggccataat
     8041 gttgaagata tttcaagaaa tgatgcaagt gctaaaaatt taaatcaaac aactaaagct
     8101 ataaataaaa ttatagacta tagtaagact cataacagaa aatcaatatg ttttatcaca
     8161 ggagttccag gagcaggtaa aacacttgct ggccttaata ttgccattga gcgacaaaaa
     8221 gtagatgaaa atgaacatgc agtcttcctt tctggaaatg gtccattagt tgatgtttta
     8281 caagaagcac tagctcgtga tgatgtaacc agaaatggaa tacgaaaatc tgaagcgatt
     8341 cgaaaagcga aagaatttat tcagataatt catcatttta gagatgaagc tatatctgtt
     8401 gatacaccac ctattgaaag agtcacaatt tttgatgaag cacaacgtgc gtgggatgaa
     8461 cctaatttga ctaactttat gaaaagaaaa aaaggcgtat tagactttaa tatgtcagag
     8521 cctgaatttc ttattagtat tttaaatcgt catataggat gggcgactat tgtttgtttg
     8581 attggtggag gtcaagagat aaataaaggt gagtcagatg gaatatcagg ttggtttgaa
     8641 tcactaagaa ataattattc tgattgggat gtttacatat caaataaaat tattgatgaa
     8701 gaatattcta aaggtaattc ttttgatgag ttaacagaag gtgttaatta tagaataatt
     8761 gatgatttgc acttatctgt atcgttacgt tcttttagaa gtgaaaatgt atcaaccttt
     8821 gttaaagctg ttttggatgt agataaagtt gctgcaaagg aattatataa tcaatttaaa
     8881 aaagattacc ctatatgtgt tactagggat ttggaattag ctaaaaagtg ggtgagagat
     8941 aaatcaaaag gttcacagcg ttatggaatg actgcaagtt ctggagctaa acggcttcga
     9001 aagtatggag tgtgggtaca aaataaaatt gatgctacaa actggtttct taatggtaag
     9061 gatgatgtac gagcttcata ttttttagaa gaaactgcaa cagagtttga tatccaaggt
     9121 ttagagctag actggacaat tgtctgttgg gatgggaact taagatttaa gaataaccat
     9181 tttgaatact ataacttcaa tggaactaaa tggcagaaca tcaataaaga agataatata
     9241 ctatacttaa aaaatgcata tagagttctt cttacaaggg caaggcaagg ctttattata
     9301 tttgtcccaa taggtgatga gagtgatatt acaatgcagc caaattttta taatggtgtg
     9361 tatgaatact taaaagaaat tggaatagag gaactttagg tttaaagtaa ggatgctatt
     9421 aagtggatta tttgtaaaaa attatcttcc tttataatat atgagaaagt aaaacttttt
     9481 aactaaagaa tacattcaaa agataataag aaaaaggaga gtaacaattt gagtttataa
     9541 ataatcttaa taattttcat ccaatctaaa atttaaatgt ggtttttact ataaacagga
     9601 cattggatta tattgtaagt tatatattag agtaattcta atttagagtt taaaagagat
     9661 tacaacttat tagtttgtat atctcttttt ttaataaaat caataatata taaaactatt
     9721 ggtatattta atgacaattc aaatattgta tataaccata atgaaagata tataaataaa
     9781 attttaaata tacttaaaaa tatattaata taatgtacat aaaaaatatc atcaatatat
     9841 gataagataa atacaatagc aacattttat taattttttg ggtttttata tatttaggta
     9901 ctggggtgat atagttgaac caagagatat tttttaaaat aatgaatgaa atattggaaa
     9961 atattgatga aggggtacat tttgttaata aagacgggaa aactataatc tacaataatt
    10021 ctgtacaaaa aatggaaaaa atgaacagac acgatatttt aaccaattca ttttttgata
    10081 tagtcaacaa aatgaaaata aacaggagta cactattaga agttttggaa aaaagggaag
    10141 ttatcaaaga caatattcag aaatatctgg ataaaaatgg aaaagagata acagcaatta
    10201 atactacaat accaattgat attaaaggcg agtttgtagg ggcacttgag atatcaaaga
    10261 atatgacaac gatagaaaat ttactaaata gaaattataa aaaaaattta gtcaataaag
    10321 ttagtagtag acaaaaaaag ggttatgaat ttgatgatat tttaggggaa agtaatttga
    10381 ttaaagaagc aatagctaaa tctaagaaag cttgcaagtc tgatgcttca gtatttatat
    10441 acggtgaaac tggaacgggt aaagagttat ttagccagtc cattcactat ggaagtaata
    10501 ggagaaacta tccatttata gcacaaaatt gtgcagcttt gccggaatca ctattcgaag
    10561 gtatcctttt tggaactagc aagggaggat ttacaggagc aatagatagg ccaggcttat
    10621 ttgaacaagc taacaaaggg acattgttat tggatgagat aaattccatg cctattatgt
    10681 tacaggctaa actattaagg gttttacaag aaggttatgt aagaagggtt ggaggaacta
    10741 aggatatacc tatagatgta agaattattg caacgacaaa tgaaagtcca aaggtaattt
    10801 taaacgagca taaaatgcga aaagatttat attataggct taacataatt catataaaaa
    10861 ttcctcctct tagagataga aaagaggaca tacctctatt gtgcaaaaaa tttatttcaa
    10921 agtataataa aaagttgaat aaaaaagtgg taggaatttc agatgaagct attaaaattt
    10981 taaaaaatta cgattggcaa ggtaacataa gagagctaga aaacataatt tattgtacta
    11041 tgagtatgat ggaagatgag gtacaaatag aaacaagtat gattaatcta aacgactatt
    11101 acaatcttaa agagaataaa gagagtaaca attattcttt aaacgatttg gaggacaaaa
    11161 cacttagtaa gattatcagt gagatagaag aaaagtatat atatgaatca ttagaaaagg
    11221 catcttacaa tataactaaa gctgcatcaa ttttaggtat gaataggcaa aatttacagt
    11281 ataaaataaa aaaatataat atcaaaatat tataaataag caagataaaa aacaaaatca
    11341 aagtaaaata aaaaagcaaa atagaggagc aaaattatta taaaagagca aaatattttg
    11401 ctcttttttt tatttctcat aagaaatatt attttttatt tatgaaaata cataataaaa
    11461 taattatagt ttcaacgttc caataaatat ttactttaat tttaaagttg gcacgattta
    11521 tgctttaact aattattgag aatatataaa tttttaggag ggtgtatata tgagaaaagt
    11581 aagagtagga atatggggat ttggagctat gggaataggt atggctaata tgattctaaa
    11641 gaaagaaggt atagaaatag tatctgtttg tagtagaagt acttcaggga aaagtatgta
    11701 tgatgtttta ggaattgaaa gaggagaaag accagaagtt attatcaata aaaattatga
    11761 agaagttttt agagaaaaaa gtgtagatgt tgttttgtta gcaactgatt catttactaa
    11821 aaaggctttt gataaaataa ttttcttatt aaatagaaaa ataaatgtta tatcaacagc
    11881 agaacaaatg gcatatccac aagctgatga tgcagattta gctaaaaaaa tggatgaagt
    11941 tgcaaaggaa aatggagtaa gtatacttgg aacaggaata aatccaggat ttgtattaga
    12001 tttactagta ttggcacttt caggtacttg tgaagaagtt actagtataa aagctaagag
    12061 agtaaatgat ttatctccat ttggaaagtc tgttatggag gaacaaggtg ttggagtaac
    12121 aagggaagaa tttataaaag gtgtagagga taagactata gcaggtcatg taggttttgt
    12181 agaatcaata aatatgatag ctgatggatt aggttggaaa ttagataaga tagaacaaac
    12241 aaaagagcca ataatgacta cagtagatag aaaatctaag tacggagagg cattggctgg
    12301 aaatgttgct ggttgtagac aatgtggata tggatatgta aatggagaag ttttaattga
    12361 aatggagcat ccacaacaga taataccaga agcagaaggt attaaaacag gagactatgt
    12421 atcaataaaa ggtataccaa acattgattt acaaataaat cctgaaatac ctggaggagt
    12481 aggtacttat gcaatgatag taaactctat tccattaata ataaatgcta gaccaggtct
    12541 taaaactatg ttggatatac cagtacctag agcaataatg ggagatataa gaaatcagat
    12601 agaagttgaa ttggaagaag aatcaaaggc taattagtaa gataacagat aactaattag
    12661 taagatagca actagctaat tagtaagata acagttagct aattggtaaa ataatgttta
    12721 atttaaaata acagtaaatt aattagtaag gcaatagtca attaataagg ggatgaaaaa
    12781 atggatgcta aaattaatga ttgggttatt atacacaata tagtattgac tccagaagaa
    12841 agagctccac aagttccaga ggatactaag aaagtatcct tagaaatgtg ggttaaaggt
    12901 tttatacaaa gtgatgcttc tataggcgac ttagtagaag ttaaaacaat tacaggcaga
    12961 cttgtaaaag gtaatttact taaaataaat ccatattata ctcatgatta tggtaaatgt
    13021 ataccagagt tactccaaat aggaatccaa gctaaagaaa tattatttgg aggtgtatat
    13081 aatgaataat tcaacatcaa tgaaagatat gagttatcaa gctgttatgg gaagaaataa
    13141 cgaaataatg aaaaatgcaa tcggattgga ttattcttct tttgaacaag agggaatagg
    13201 gtttgattat gagaaaatga tgagtgaaac aggatataca ttacaagata ttgaagcaat
    13261 tcaatcacag tatgctgttg gaaatactcc tttaatagag cttaaaaatt tgactaaatt
    13321 agctagaaaa tgtgctaaag aaggtaaagg agctagaata tttgtaaaag atgaagcaat
    13381 gaatgcatct ggtagtttta aagcaagaag agcagctact gcagtatatc atgctaaaca
    13441 aatgggatat aaaggtgtaa tagcagctac tagtggtaac tatggtgcag ctgtagcatc
    13501 acaagcggca atgcagggat taaaatgtat aatagttcaa gaatgttatg attctaatgg
    13561 tgtaggtcaa cctgaaatta tagaaaaagc acgaaaatgt gaggcatatg gagcagaagt
    13621 tgttcagtta actgttggac cagagttatt ttatacgttc ttagttttat tagaagaaac
    13681 aggctatttt aacgcatcat tatattcacc atttggaatt gcaggtgtag aaactttagg
    13741 ttatgagtta gctatccaat ttagagaaaa gtataaaaaa gaccctgata tagttgtttg
    13801 tacaaatgca ggtggtggta atcttactgg aactgcaaga ggattgataa aagctggttc
    13861 tattaatact aaagtagtag gagcaagtgt agatttaaaa ggattacaca tggctagtga
    13921 taatcaattt aataaaaaat catttacaac aggacataca ggttttggga taccatactg
    13981 tacatggcct gataggtcag atgtacctag gtctgcagct agaccactta gatatatgga
    14041 tagatatgta ttagtaaaac aaggagaagt tttttataca actgaattat tggcacagct
    14101 agaaggtata gaaagaggtc cagcaggaaa tacatcttta gcagcagcat tctctttagc
    14161 tcaagagtta gatgaagata aagttatagt ggtacaagaa acagaatata cgggtgcagg
    14221 taaacatata tgtccacaac ttacctttgc aagagaaaat ggaatagata taaaatttgg
    14281 aaatccaaga gaagaaattg caggagtaaa cttaatatta ccagaaagac cagaattatt
    14341 gaaatgtgtt gatattgata tgaataaaat aagaaagtca tttataaaaa attgtatatc
    14401 taataatcac attgataata ttgataagct aagtaatcga gatatagaat ttttaatgga
    14461 agaagtaaaa agttcaagag attttgttgt tgatgtatta aataatttat agtaattatt
    14521 gttttttaat ttaatataaa ataaaaattc agaaaattcg attaaatcaa tacgaatgga
    14581 ggaatgttca tgaaaaaaag agaggatgat tttgaagtta gaagaaaaca tcttcaagaa
    14641 ctgagtgagg atgagttaaa agaaaggttt tggagtttag ctacccagat agtagaacca
    14701 atgatagaac ttggtaaaaa gaatacaaca ccatcaatag aaaggtctgt cttgttgaga
    14761 atgggatttt cttctcttga agtaaaacct attttagaag gggttatgga aagaggtctt
    14821 ataggtaaag gagcagggca tgtagtttat aaattggcta aatctaaaaa tataacggtt
    14881 agagaagcag gtttgttatt agttaagggt gaatattggg atgaagttac atgtttattt
    14941 agagaagggg tagaatcatg ttaaaggaaa ataaaaaatt ggatatagac tatatattaa
    15001 aagatttgga taaatataaa ccaaaacgaa gaggttgggt ttggagagaa cacttggaaa
    15061 atcttgaaat gggacctttt aaatataaag actgtacaaa acctttaaag aagagtgtag
    15121 ggctaccatc atctaagtat ttcaataata tagaccctca accaagtcca gttattacta
    15181 ctgaaatagc ttctggtaga tttgaggatg atataagaag aatgagaatg gcagctcatc
    15241 atggagctga ccatctaatg gttatcagaa ctgctggaca atcacatttt gatgggctta
    15301 tagaaggaac tccacaaggt ataggtggag ttccaataac aagaaaacaa gtaagagctc
    15361 aaaggaaggc tcttgatttt atagaagaag aagttggtag acctattaat taccattcat
    15421 atgtaagtgg tgttgctggt cctgaagtag cagtaatgtt tgctgaggaa ggagtcaatg
    15481 gagcacatca agacccacaa tataatgttc tgtataggaa tataaacatg gtaaggtctt
    15541 ttgtagatgc ttgtgaggca aagaaaataa tggcttttgc caatatagct caaattgatg
    15601 gagcacataa tgctaatgct actgctagag aagcatggaa ggtaatgcct gaacttatgg
    15661 ttcaacacgc tttaaactct atattctcag aaaaaatagg tatagataaa agtaatatat
    15721 gcttatcaac agttccacca acagcacctc cagcaccatg tttaaaaata gacttacctt
    15781 acgctgttgc acttagagag ttatttagtg attataagat gagagctcaa atgaatacta
    15841 aatatatgga atcatcaact agagaagcaa cagttactca tgtattaaac ctattaacat
    15901 cagtattaac tagagctgac atacaatcaa ctataacacc tgatgaaggt agaaatgtac
    15961 cttggcatat atacaatata gaagcatgtg atacagcaaa gcaagcttta gttggtatgg
    16021 atgggcttat ggatatgata gaacttaaag atgtcggtga attaagagac aaagcaagag
    16081 aacttaaaga aagagctgta ttatatatgg aagaaatatc tgaagttggt ggatattttg
    16141 aatctgtgga acaaggattt tttgttgatt ctggaaatta tccagaaaga aatggtgatg
    16201 gaatatctag aaaaataaaa ggtggagttg gtgaaggtac tgtttatgaa agagaagaag
    16261 attatttagc acctgtaact gctcactttg gatacaataa tgtagctcaa tatgatgaaa
    16321 atgctataga taatccatca atgttaatag atggatgtac atttgaaaat ccagacaaaa
    16381 taatatatat tgatgagctt gacgattttg ataatgtaga aaatagacta aaagaatcat
    16441 atgagtacag aaatggaact aaaattaagc ctgaaatgga atggtgtgca gatggaattg
    16501 taatgataac aatgatgctt cctactgata aaagaacagc agagtttgca gctttggaat
    16561 ttgtcaaaaa aatgaactta caagaaattg aggtcataag tagagaagtt atgcatgaat
    16621 ctgaagggac tagaatagaa gtgaaaggaa gagttccgtt tgatatagat ttaaacaatt
    16681 tagtaatacc agaagaacct aaggtactaa ctgatgaaga aataagagca gatatagaag
    16741 aaaaacctat gaaaatagtt tcagctacgg ttggagaaga tgaacattca gttggtttaa
    16801 gagaaataat agatataaaa catggaggta ttgaaaaata tggcatagaa tgccactatc
    16861 tagggacttc tgtacctgta gaaaaattgg tagatgcagc gattgaactg aatgcagatg
    16921 caatacttgc atctacaata ataagtcatg atgatattca ctataaaaac atgaaaaaat
    16981 tacacgatta ttgtgtagaa aaaggtataa gagataaagt aatgattgcg tgtggaggaa
    17041 cacaagtaac acctgaaata gctgttgaac aaggcataga tgctggattt ggaagaaaca
    17101 gcaaaggcat acatgtagcc actttcttag ttgaaaaaag aagagaaatg agtaataaat
    17161 aatggaaaat agcaaagttt ctttaaaaat agatgtgttg gtagctgaaa ttggaagtac
    17221 tactacagtg gtaaatgctt tccacgatat taatactaac aatcctatat ttttgggtca
    17281 aggtcaagct ccaacaacag tgtttgaagg tggcgatgta agaaatggct tatcaggtgc
    17341 tattaaagat ttagctaata aattatctgt agatgatata gagtataatg atatgtttgc
    17401 aacttctagt gcagctggtg gattaaaaat gactgttcat ggattagtct atgatatgac
    17461 agtaaaagca gctaaagaag cagctttagg ggctggagct gttattcgtc aaataacatc
    17521 aggtagaatt aaaagaactg atttaaataa aataaaagaa ataaacccaa acataatttt
    17581 aatagctggt ggagtcgatt atggagaaag agatacagct atttataatg cagaaatgat
    17641 agcttctatg aatctaggca taccagttat atatgctggt aatgttgaaa atcaagagga
    17701 aataaggtta atatttgaag atactaatta taagctttat ataactgaaa atgtataccc
    17761 taaaatagat ttattgaata tagaacctac aagaagaatc attcaaagtg tatttgaaga
    17821 acacataact acagcaccag gaatgaaata cattaaagaa atggttaatg aaaatattac
    17881 tccaacccca ggagctgtaa tggaagcttc taaactttta tataaaaata ttggagattt
    17941 attaactctg gatgtaggtg gagcaactac tgatgtacat tctgttacag atggaagtga
    18001 ctatattaat aaaattttgg ttaatcctga acctactgca aagagaagtg ttgaggggga
    18061 tttaggtgta tatgtaaata tgaagaatat agtagaagtt ataggtaaag aaaacttaca
    18121 aagtgaacta tctatagata ttgatgcagt aatagagaat tatcctccaa taccaaaatc
    18181 aaaagaagaa atattatttg ttgagaggtt gacaaaagaa gctgttgtta aagctatgct
    18241 aagacattca ggaaaaataa gaaatatcta tggaacaact ggaaaagtca aaattgcaga
    18301 agggaaagat ttaacagaag taagatatat agttggtaca gggggagcac ttactaggct
    18361 ccctagtaga attgaaatat tagataatat gctcaaatac aataaaaata atgaattact
    18421 atttccaaag gaaaaaacaa agatattaat agataatgac tatattatgg catctatggg
    18481 tgttttatct aaaaaatatg aagaagcatc tcttaagctc ctgttaaaaa gtttgaattt
    18541 tgaggaggaa agattatgta tcctaggtta gaaatagata tagaaaagtt aaagcataat
    18601 gcaaaattaa tttcgcagat gtgtcatgaa agaaatatta agatatcttt tgttactaaa
    18661 tcattttgtg cacaaaaaga aatagtagaa gaaatatgta gtgaaggaat agaccatata
    18721 gcagattcaa gaatacaaaa cttaaaaaat ttacaagata taaatctacc taagatattg
    18781 attagaatcc caatgttaag tgaattagaa gaagttatca attattgcga tattagtttt
    18841 aactcagaat taggaacaat aaagaaatta aatgaattat gtgaaagtaa aaatattatt
    18901 cataagatag ttcttatgtt tgacttaggc gatttacgag aaggatactt ttatgaagaa
    18961 gactttttta gtaatgtaag agagatagta agattaaaaa atattgaaat aattggtata
    19021 gctacaaact taacctgtta tggagctatt ataccatcta gggaaaatat aggcagactg
    19081 gtaagtatag caaaaagaat ggaagaaaaa tttggaataa atctagaaat agtatctgga
    19141 ggtaattcaa gttcgattca tctgttgata aataataata tgccagaagg tattactaat
    19201 ttgagaatag gcgagtccat tctacttggt agagaaacgg catacggtga aaatataaat
    19261 ggaacatatc aagatgcatt taaattaata tgccaagtag ttgaatgtaa agaaaaacca
    19321 tcagttccta ttggagaaat aggagttgat gcctttagaa ataaacctgt ttatgaagat
    19381 aagggaatct taaaaagggc aatcatagct ataggtaaac aagatataaa tattgactct
    19441 ttaattccaa tagacactga tataaaaata ttaggggcaa gttcagacca catgatacta
    19501 gatgtaagta acacaaaata tgattataaa ttaggtgaca atttagaatt tttattaact
    19561 tatgggggta tcatgtcatc tagtacgagc aaatatgttg caaaaaaaat tatagtgtag
    19621 tgtttaaata attaggagga aactatttta tgaaagagcg agaatgtaga aaagcaagtc
    19681 tgtttgatgc aatgatacca attacttgtt tgatattatt tttaagtcta ggagtattag
    19741 tgtatggttc atcacctcat gtaccactaa taggagcagc agcagtagca ggacttgtgg
    19801 ctgtatatag acttggattt aaatggaaag agttagaatt aagtatgttt aatagtataa
    19861 agatggcaat gcaagcaatc ttaatcataa ttataatagg cgtacttata ggaacttgga
    19921 tagtaagtgg agtagttcca tgtatgattt actggggact aaaaatttta tcaccaaata
    19981 tttttttagt agcatcaaca ttagtctgtg caatagtatc tcttgcgaca ggttcatctt
    20041 ggagcacaat gggtacagta ggagtagctc ttttaggaat tggtcaaagt ttaggtatgc
    20101 caattggatt aatcgtaggt tctataatat ctggagctta ctttggagat aaactatcac
    20161 ctttatctga aacgactaac ttagctccag caatggcagg aacagatttg tttacacata
    20221 taaaatatat gctatattcg actataccat cattattaat ttgtttagta atttatggtg
    20281 taattggaat gaagtattca ggtcaagcac tagatataaa acaaatagaa ttaattaggt
    20341 ctacattaga ttctacattt aatactttat caccagtatt attattagct ccagcaatag
    20401 taattggatt agttgtattt aaagttcctg ctataccagg gcttatagta ggtgctgtat
    20461 tgggagtatt gtttgcagta atttttcaag gagaatctat gaatactata ttagatgcag
    20521 cacaaaatgg atatgtttcg tcaacacaaa tacaggaggt agatgcttta ttatctaaag
    20581 gtgggattat gaatatgatg tccactgtat ccttaacaat atgcgcttta tctcttggag
    20641 gtattttaga aaaaacaggt atgcttgaag tagtagcatc ttctttacta agattagcaa
    20701 agggtgtatt tggaacagta ttatgtacta tggtaacttg tacaattacc aatataatag
    20761 caggtgaaca atatctatct atagttatac caggaagaat gtataataaa gaatataaaa
    20821 agagaggtat acatcctaag atgttgtcga gagctttaga agatagtggg actttaactt
    20881 ctccattagt accatggaat acatgcgggg cgtatataac agcaacatta ggtgtatctg
    20941 cactaacata tggtccatac gctttattaa atattataaa tccaatagta tctttagtat
    21001 taattgcatg taagtttaaa atagcaagaa tagaagatga acctgaaact tgtttaaatt
    21061 ttgatgtttg ataatagata acttatgtaa gaacgtagga actagggggg tagcctgtgg
    21121 ggaattagta agacatattt tagtgtgcga aattctataa atcagtaatc ttgtgacttt
    21181 agccatggga ggttcagatt agtaaattta tagttttata ttagtctata ttttgatatt
    21241 ttaattgttt gtataaattt tttatagaaa aagatttctt ttagacttgc ctaaaaatta
    21301 ggcaagtctt ttaagtatgt tctgattata agagcactgt gtgacaagaa tgatgagatt
    21361 atttttgcta tactttcgat ataagaaatg gaaggaggtt atgttttata ttcatatgaa
    21421 ataattagtt acacagtgaa ttttcgtata atttggtata aaaagattag aaatgaaaat
    21481 aaagtaatgg aggaataaaa tgactagaat atccaatata acaatagctg aacaatctga
    21541 gtactgtttt ttggcaatac gaaaaacaat tgactttatg gtagaatttt cagagttttc
    21601 aaaacaaagt tttgaaaaaa tttctaaata tctggaggag agaggaattt tatcttcagg
    21661 agcaccgatt gtatgttttc ataacatgga tttagtaaaa ttggatgttg aagtagggtt
    21721 tcctgtagcc acttatatag atgggaaaaa tgagattcta caaaagattg ttcctgcaca
    21781 gaaaataata actgcaattg atttaggacc ttatgaatta caagacccta ctttagaaga
    21841 cttattttca tgggcaaata gcaatggata taaacttcat ggcgatatat attaccagta
    21901 tttaaatgat atcaatcgtc cagctagtga atatcttacg aaaatgatgt tacccattat
    21961 atagaaagtt aaagattatc gtttgttagt tgataattag aagaacattt actgctacta
    22021 cataaatttt atcctaaata atatcaaggt gatttaattt atgtattcta atgtaacccc
    22081 aatatattgt taaggtctac ttaagatgct caaaaggcta tgcttaattt attgttatat
    22141 aatttgtaga gtggtataat ttagaggtaa tattatatag atatataggg ggaaaatatg
    22201 aaaatatatg atatattaac acgtttttat attcaagata tagaaaaagc catacctttt
    22261 tatgagaatc ttttaaaaga aaaatgttct ttgcgttttt cctataaaga agttgggttg
    22321 gaattagcgc aaataggaaa tgttttacta ttatctggtt ctgatgatgc tcttaaacca
    22381 tttatagaaa caaaatctac ttttatggtt gattctgttg atgaatggag aagttatcta
    22441 ttggataatg gagcagttgt agtaagagat aagaaaaaag ttcctacagg atataatatg
    22501 acattgagac atccagatgg tacaattata gaatatgttc aacatacaaa gcaggattag
    22561 ttaaaaacag attttactac tgagcaacta attagaatct attcttagac gtacaattta
    22621 ttatttttgg tattaacaga ttataagtca aattataata tttcaaaata gaatagaaat
    22681 ttatttacat aaaagagagt gtttaaaaat gaacttttta gttcatgaag cactcttttt
    22741 tgtatgaaat caaatacatc aatatataaa acaaactgat gaagagctta aattaaaata
    22801 aaaagattca taacaggacc ccattaaact ttaagatagg gtaagtttgt aatcatatta
    22861 aataattgta aaaagctgtt tttagtcgta taatataaat atttatcatt taattaagga
    22921 atctaaaagg aatctaataa gaagaaaata ataaataact ttttagattt actaaatgaa
    22981 aaattattga aaattaaagg attagagtct ttaatcagaa agtaaaaagt aatatattaa
    23041 atttatgaat tgaaaactta aacaacaaat ttgtttataa aatagcaaga ttcattaaat
    23101 ataactaagt aataattgat ataatgttgc atggataaaa ggagggaagc tgcaatgtct
    23161 tacaaagtaa ttaatgtcat aactgctatt gactatatag aggagcattt atcagaaaag
    23221 ctggatttag atatagttgc taatgctgtt cattactcaa aatatcacct gcatagaact
    23281 ttcacaactt ctgttggtct aacaatgcat gattacataa agcggagaaa gttgacagaa
    23341 gcagcaaaat tattggtatt ttcaaagaaa ccaattattg aaattgcatt gatagctgga
    23401 tatgaaagtc aacaggcatt cacttctata tttaaagcta tgtataaaaa atctccaaat
    23461 aaatatagga aagaacaaga attttatcct ttgcagctaa gatttgtttt gaaaaaagat
    23521 aacctatttt caaagaatgt actagagttg gaaaaagaaa ttaaacttgc aagtatgtct
    23581 gacataccat tatggatgaa tttagtgaga ttagtcatag atggatttcc aaatttgcaa
    23641 gaggaagaat atatatatca attagaacag tatattttag aagaaagagc tttaatatta
    23701 aaacttaata atgtcgctat tgcaaatatg gtgtttaata gagaaactag aagtattgat
    23761 ttttttggaa tacatccaca gtatcgtaat tcagacattg cacaagtgtt tttgaagaaa
    23821 gttattgaag atttcttgat agatacagat atttcaatta ctacctttcg agagggtgac
    23881 aaagctgata caggacatag agatatgata aagaagttgg gctttgcaga agcagagttg
    23941 cttgtggaat ttggatatcc tactcaaaaa tttattcttc ctttaagtta aatagcaaga
    24001 atgattaaaa actccaaaac acaattaagt ataatgattc agtgtaagta cttaattaac
    24061 aaaggaggat ttaaatgtct gaaaataaac aagtagtaaa ttgcgatatt attgcatcta
    24121 attctaaaca taatatgtgt gaatatgatg cagagttatt aacttttttt ccagatgaga
    24181 ttgcacatct aatagagata aataacaagc tagacaatgc ttttaagaaa gctgaaaatc
    24241 ttgttgacaa gcttgataag gattatatgg acgctaaaat gtatatggta aaaaatcgtg
    24301 gtgaaataga cccacatgaa atgtttcaaa atgaacaagg actaaaacaa atagataact
    24361 atggggcttt tatggtaaaa gttcgagata agattaataa aatcaaagat tctccatact
    24421 ttgctagaat agattttagg ctgaaagata tggatgatga atctaagtat tatattggta
    24481 gatttgcatt tgattatgaa gatgagctta taatacttga ctggcgttct ccaattgcca
    24541 gtatgtttta tgattatgaa atcggaaaag ctggatatga tgctccaata ggatgggtgg
    24601 atggagaaat aacaagaaaa cgacaattta aaattaaaaa tggaaaatta gaatatgcac
    24661 tagaaagttc cattaatata caagatgata ttcttcaaaa agaacttagt cacacttcag
    24721 atgaaaaaat gaagtcaatc atttcaacaa tacaaaagga acaaaatcaa attattagaa
    24781 atgataaagc tgatacactg attattcaag gggtagctgg ttctgggaaa acatctatag
    24841 cattacacag aattgctttt cttctttatc gatttaaaga taaaatatca gcaaataatg
    24901 taattatcct atcacctaat aaagtctttg gagactatat ttccaatgtc cttcctgagc
    24961 ttggagaaga accattatgt gaattaagtt ttgaaaatat agcagaggta caattagata
    25021 gagttattaa ttttgaaagt gagaaagacc cattagaaat aaatgatgct aaatggtctg
    25081 aaagagtgcg ttttaaatct acacttgatt ttgtaaaact tattgatgat tatataaaac
    25141 aaatgccaaa taagattttt attccaaaag actacacttt tggaagtttt acagctaaaa
    25201 gtgactggat acagagtaga tttgaggcat ataacagata tccagttaag aagagattag
    25261 agaatgtagc tgaagacatt cattataaat ttgaatcaga taatattatg gaagaagatt
    25321 taccaaaggt aaaaagtata cttaaaagtt tgaatggaat gttgacaata aaaaatactc
    25381 ttacactata taaggatttc tttaaacaaa tgaatatatc aaacatgttt gtgatggtag
    25441 aaaagaaaac acttgaatgg tcagatgtat acccatttat atatattcat gctgcatatg
    25501 aaggtataca agaagataag attataaggc atattgttat tgatgaaatg caggattata
    25561 cacctattca atatgctgta ataaacttac tttttaagtg taaaaagact attcttggag
    25621 attttggaca attggttaat cctaatcata ctcatacact agatgatatg aaacagctat
    25681 ataatgatgg ggaattagtt atgttgaaca agagttaccg ttctactttt gagattatta
    25741 atttcgccaa aaagattcaa gacatatctt cattagagcc tattgaacga catggtgaag
    25801 aaccagcttt attaaaatgt aataacgagc aagatgaaat aaataagata aaaatagaaa
    25861 ttgaagaatt taaaaagagt gataatgcga ctcttggtat tatattaaag acagataatg
    25921 acgctaaagc tgtatatgat gcgttaagag aagaatatag tgttcactta atatcttctg
    25981 aaagttctag ctttacaaaa ggagtatcca taacttctat taaaatgtca aaaggtttag
    26041 agtttgatga agttattgta ccttcagtaa ataataaaac atattatagt gattatgacc
    26101 gcagtttgtt atatattgcc tgtacacgag ctatgcataa acttaagttg acatatactt
    26161 gtgaattgac tcaacttata gatatgtaga ttatatactt attatgtaag tgtttactta
    26221 tataaatata acctgtcaat aaaattcatt aataaatcaa taaaaacaat atttgtaaaa
    26281 gtaaaagcta taaattatat attaaatttt ataatttata gcttttataa tactgcttat
    26341 cttaatcctc ccaatagcaa aataatttta aacatgccat attttataaa tgtaataaca
    26401 attatacaat taaaataact ttcctaaact aacttgacat ttaaaactta ctattgtaat
    26461 atttaaatat tacaatagta agttttaaat gtggaatcaa ttattatatc acaaattaaa
    26521 aggaggacag gatgttatta atagttacct aattatcttc gcaataggtt taatcaacat
    26581 aattgtacta ggtgaagaaa atataagaga tacagctatt catttagtct tgtttcttat
    26641 aagtagaacc tagattggag tatattacaa ataatgtcta ctattttaat agtaggttat
    26701 aaatttaaat atgggagatg atataaatta tgaaaagaaa aaagaatttt atttggatag
    26761 ctatcttatt ggttggtgta gttatggtaa tgtattatgt agggaaaaaa cataacgata
    26821 taaatcaaaa aactgataag aattatataa aatctgaatt aaataagtct gaattaaata
    26881 aatccaaaat tgaaaacaat gataaaaaca aaaaagaaaa aattaatatg gttgactata
    26941 gtgattgttt tgaaggtatt agtggaggag ctatttttta taatactaaa aataaagaat
    27001 ataatatata taacaaagaa ttaattgaaa caagaaggtc accatgttct acttttaaaa
    27061 tagtttcaac tttaattggt ttagaaaaag gggtaataaa ctcaaaagaa tcagttatgg
    27121 gatatgatgg tacagagtat ccaaataaaa attggaataa aaatcttagt ttagaagagg
    27181 catttaagga gtcttgtgtt tggtattaca aaaaattgat agataaagtt gatgctaaat
    27241 ctgtccaaaa cattttagat gatttaaagt atgggaattg cgatattagc gaatgggaag
    27301 gcgatttaaa aaatggaaag ggacatttaa atggattttg gttagaatct tcattacaaa
    27361 tatctccaaa agaacaagtg caaacaatgg caaaaatatt tgagggagat acaaacttta
    27421 aaaaagagca tattaatatt ttaagagata ttatgaaaat agatataaat gataaaaaca
    27481 taaatgtata tggaaaaact ggaacaggat ttgatgaaaa aaataaacgt gtggatgcat
    27541 ggtttgttgg tatgctagag cgtgaaggtg acacctatta ttttgctatt aaatcagatg
    27601 attccaataa agaaattaca gggccaaagg taaaggaaat tgcaattaat ataattaaga
    27661 aatattatag tgtaagggaa taaacttaat tttatttgta aatattattt acctatagtc
    27721 tgtaatattt attaaataat tttactaaat tgagtctata tagagcaaaa ctcctttagt
    27781 acataaaata cttgctattg attattaaat tgaataccta tagatttaaa gttaatacct
    27841 tttaaagact atgtaatata ttaaaagtat aaataaaata tattgcatag tttttgtttt
    27901 atattataat tttaaataat gtgcaaaaac taaacaatat ttttaatagt aatttatatt
    27961 tgtattcatg tcactataat gtcattttag gttgctaaaa tttaaccata aaggagatga
    28021 ttgtatatgg aaatattaaa atgtgaaaat ctcacaaaaa tatatggttc aaatcaaacc
    28081 agagtgacag cccttaataa tgtaaattta tcagttcaaa aaggtgattt tgtatccatt
    28141 gtaggagcat ctggttctgg caaatctaca ttgttacatc tgcttggggg agtagataga
    28201 cctacatctg gaaagattta tgttgaagat acagaaatat caagtttaaa ggaagaagct
    28261 cttgcagttt ttagacgtag aaaagtaggt cttatttatc aattttataa tcttatacct
    28321 acccttgatg ttagaaaaaa tatattatta cccatgttac ttgataaaag aaaggtggat
    28381 gaggatagat tctctgagat tgtatctata ttgggattat ctgatagatt aaaccatctt
    28441 ccaagtcaac tttcaggtgg acaacaacaa agggtatcta tagctagaag tttaatctat
    28501 agaccagcta ttttattagc agatgagcca actggtaatc ttgatagaaa aaattcagaa
    28561 gaaattgtag acttgttaaa cttatcaaat aaacgattta atcagacaat acttcttatt
    28621 actcatgatg agaaaatagc actagaggca aatcgtattg taactatgga agatggagtg
    28681 attgtctctg aaaaggtggt gaagaaatga atattcttca agaatacacc ttagactttg
    28741 tacgacgcaa taaacgtagt agtatagcta taatgttagc tatattactt actacaacca
    28801 tgatgtcttc tttgtgtgga ctgttgtata ccatgtggac agattttata agactttcaa
    28861 ttgagaaaga aggtaactgg catggtgaat tgtttgacta tacttatgga agtgacttac
    28921 cattaattga aaatttttct tcagttgatg atgtaatggt aaaaggacaa tggtatgtag
    28981 gcaaaattaa tgatgagaaa agagattata taatttatag aaatgctaca tcagagtatt
    29041 ggaattctat gccagaaaag gatactataa tagagggtcg tcgaccaaat aatgctaatg
    29101 aaattgcatt atcaaaacaa tattttgaga ataatccaag tgtaaaaatt ggtgataaaa
    29161 ttactctccc aataggatat agaatcttaa atggaaagcc tcttgaaccc atagccccaa
    29221 aatcgaaagg ggaaagtttt aaaaaaattt cagaggctac attgacaata gtaggaaagg
    29281 tagatgtttc tacttcatca gtaatacctg catatacagc tcttggatat cttgataaaa
    29341 caagtataaa acctaatgat agtataactg tgtacttgcg atttaaaaat ataagagaca
    29401 cgtataagga acttccaaaa cttgctaaat cattgggatg gaagactaat gaatatggaa
    29461 aatacaatct taaatacaat agtaattatc ttttaaagat gttagtcttt tcaccagaac
    29521 agaaagcatc tatgagtagt atatcaaaat tttcaactcc tattatgtat cttacaatag
    29581 caatttttac agtagccgtg tttgtaatgg tgatttacaa cgcgttttca ctgtctgcaa
    29641 atgctagact tactcagtta gggatattat ctagtgttgg tgcatcacca aaacaaatta
    29701 aaaggtctgt agtgtttgaa ggctttttac tgacaattat accattacca ataggtttat
    29761 ttttaggatg gttattgtgt aataggctta tagtttatgt taattcagtt aattatcata
    29821 ctgatgttcc agaagtggta tttacttatg gtattcctgc ttttctgcca gcagttttac
    29881 ttacaatagt aacagtctgg atttctgcac ttataccagc acgaaaagta tcaaagatat
    29941 ctcctataga agcaatacga caaggagata gtgttaaaat caaaaagtcg cataaatatt
    30001 cattaggaaa gatatttgga atagaaggag aacttgcatc aaacgcacta cgtgctcgta
    30061 aaaaatctta tcgtactgca acaatatctt taacattatc ttttttatta cttacatgtt
    30121 ttttgcatat aattgcaaat caagaaggag caaaagctgt ttatggaagt gataattata
    30181 aggaccaaag agatatagca gtttatttgg aaaatagtga acctgtagag tctgctttca
    30241 ttgaaaaatt agataataca gaaggtgtaa aaagttcact atattatcaa aaaatgtctg
    30301 cttccacttg gctgactgaa aaagatgcat caaaagaact tgcgaaaaat ggaggattta
    30361 aaagtatagt ttcatctaaa aaatactctc ctatagaacg tgatggcaag tttcgtataa
    30421 tttcaaatat aattgggctt gatgataaga gttttacaga atattgtagg caagtttctg
    30481 tagacccaaa gttattttat aatacaaaaa atccaacctc aattgtatat aataaggagg
    30541 aagatattaa tcgtagtaca cgtagaaata aagtatatat agattattta aatctttcaa
    30601 taggagataa cattaaatta aatgaaaaag tatatgacga agataaaggg gattatactt
    30661 acaacatgaa agtgggagct attacaagca cacttcctaa aatagtagat actagttcaa
    30721 agtatacatt aatgcaaata gttccaatgt ctatggtaca ggaaatatct aaaaattatg
    30781 atgaagtaag taggttacgt tcaaataggc tgataggagt atttaaagta aataatcgtg
    30841 aggacattcc tagagtaaga gaaaaattag agtccattta taataaagac tatggttcag
    30901 gagattatag tattgaggat gttttagaaa gtgaaaaaca aaaggctagt ggaataaaga
    30961 ccatgaattt aatagtaggc tttattacag gtttattggc tatgattgga ttatcaaatg
    31021 tacttgctac agtgtcagga aatattagaa gtagacgaca agagtttgct atgttgcgtt
    31081 ctgttggatt atctccagaa ggcataaaaa agatgcttat tttggaaggt ttattcttag
    31141 gaattacacc attactactt agtattcctg tgcaaatagg aatagtgtat gcttttctac
    31201 gtataaatga aatatatttt atagagtatt taccatttgc acctatatct acaataatag
    31261 gttttactat attgatactt tttattgtaa tagcatcata tatgacagga tacaagcagt
    31321 tgaagaatga aaatatagta gaatctataa aaaatgaaac aatataaaca aaatctattt
    31381 gaacttattt aaaggtttcg atttaagact acttacaagt atagggatag ttaagttgtt
    31441 tgtttaataa aaccttaaat gtaaatagaa gtatattact aaaacaatag ataacataga
    31501 atatagatat agctggatta taggtatttt acctataatc cagcatttta tgtgtaaaat
    31561 ttaattatga atcttgcacc accattatgg caattttctg catgtagagt accattttga
    31621 cgttctataa tagactttga tagagcaagc cctataccaa tactgtcctt actagcattt
    31681 ttaccacgat aaaatctatt aaaaatatat ggaagttctt cctgagcaaa ccccttacca
    31741 ttatcttcta tgataatctc aacatataga ggattttgac tataataagc aactatatat
    31801 ccaccatctt ctatatgctc actacaattt ttaagaatat ttaaaagagc ttctaaagtc
    31861 caattaatat cgatttcaaa ttctaccgaa ggagaagcct cgatagtaaa tgtctgatgt
    31921 ttttctctta aagaaccttc tataggctca attgcataag taagaagaga atgtacatct
    31981 ataagtttgc tttcaaatat taaagtactt gcatccaatt ttgataatgt aagtaaagat
    32041 gaaactagtc tctcaagtct tgagatttga tttgtaagtc tgttaatata atctatttct
    32101 tcatctggac aattttctga aagtaattgt gccataagtg acatagaagt aattggagtc
    32161 ttaagttgat gagcaatatc tgcaagatta tctgaaagag tttgtctttc ttttaaagca
    32221 aattcttttg aacaacgaag ttccccaact gttttatata tctcatcttc aaggtgagaa
    32281 aattcatctt cacatcgtaa aagtacagac tcttttccta aattcacttc ttctaagtat
    32341 tgagtaagat tgtttattct actcagtttt tgcttatttg ttctatgttt gattatatat
    32401 ataactgtag ccattgttga aattatacca aacgatatca ctagaaaata aaaaaagttt
    32461 ttactccaaa atgtactttt agaatatcca tgttttgata aaatttcatt tcctgtttta
    32521 aaatccttat cactagaatt tttcagtgat tttagtatac tttctgtaga cttaggattg
    32581 tactcatatg atgcaccaag agccttagac agtacattgt attcattaaa agaagttata
    32641 gttgttagaa ttacactcaa taaaaaagtt acaattaagc agaatattat aaaaacaact
    32701 gtactgttga tatttcttgg agttttcact agctgtcctc cattctatat cctatgcctc
    32761 ttaacgtttt tatacaaggt ggatttttta atttttctct aagacgtttt attgtaacag
    32821 taagagtgtt atcatttacg aaattaccat ctacatccca gagcatttct agtaactttg
    32881 tacgagtcat agctctattt ttattttcca taagacttag aagaagttta tattctcctg
    32941 ctgtcaaagg tacttcaaca ttatctaaat atgctttgac ttcaacctta ttcaagctta
    33001 tattttcaca tgtaatagta ttgttagtat ttgaatgagt tcttctaaga actgcattaa
    33061 ttctggactg aagtactgag agcaaaaacg gtttaaccac atagtcatct gcacccatat
    33121 ctaatccacg tataatgtct ttttcatcat cacggactgt caaaaatatc actggagtat
    33181 cttctatact tttaacatat tggcaaaact cgtatccaat accatcaggt agatttaaat
    33241 caagcagaat taaatcaata gttttatcaa aatttttttt agcttcagca atcgtattat
    33301 aacaacatac agtgtgattt ttcttttcaa gggaagtttt tattccaaat gtaattgtat
    33361 catcatcttc taaaagaagt attttagcca tttactttta atcctttcag taatttagtt
    33421 attccattaa ctaagttatt agttttatgt ttaatatttt ttatattaga gatagaaaaa
    33481 attatcattt aagtatataa actttaattt ataaacttag gtttatgctt taattgaatc
    33541 tattaaaaga gcaagagttg tttcaataaa tttttcctca tctatattaa agttgttcag
    33601 gtatgatttt cctgttacag atagtaaatt aaaaaatcct gtaagtgtaa atattgatga
    33661 atacataaga gtaggtattt caatatcatg cctaatactt ctatcatttt ttccaatttc
    33721 aaacattcct tgtatttcat caaacataaa tttattaaaa gaaaaaaact tttctttaaa
    33781 cagcatatca gtatccgatt tttcagagtt tacaatacca atcattccca taaggcataa
    33841 tagagaaggt tctacagagt aaaatttatg aaaagcaaag tatgaaagtt ttattttttc
    33901 aaaccctgtt ttgccttttg cactttctat tttaacatta tcccaaagtc ttttatatcc
    33961 tcttaagaca acagcaaaga ataaatcttc tttacatgaa aagtacttgt atatggtgcg
    34021 tttagtatat tcaacttctt ttgctaattc attcatagtt gtattatcaa atccattaag
    34081 acaaaataat ttttctgcct tatcaataat ctcattttct ctttgtattt tttctttatc
    34141 tctttttgat aattctgaca tataaattat tcctttctat tgataaaaaa gtatactgat
    34201 ggtatactta tgtaaatata atacttgtat acctatagta tactttaata aaa
//
LOCUS       DAMJYC010000038        32412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJYC010000038 DAMJYC010000000
VERSION     DAMJYC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.039"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(286..1125)
                     /locus_tag="PC472_001950"
     CDS             complement(286..1125)
                     /locus_tag="PC472_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2727276.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
     gene            complement(1149..1907)
                     /locus_tag="PC472_001951"
     CDS             complement(1149..1907)
                     /locus_tag="PC472_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2727277.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1954..2427)
                     /locus_tag="PC472_001952"
     CDS             complement(1954..2427)
                     /locus_tag="PC472_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727278.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2456..2872)
                     /locus_tag="PC472_001953"
     CDS             complement(2456..2872)
                     /locus_tag="PC472_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2727279.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2919..3971)
                     /locus_tag="PC472_001954"
     CDS             complement(2919..3971)
                     /locus_tag="PC472_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2727280.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4367..5047
                     /locus_tag="PC472_001955"
     CDS             4367..5047
                     /locus_tag="PC472_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2727281.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5180..5500)
                     /gene="sugE"
                     /locus_tag="PC472_001956"
     CDS             complement(5180..5500)
                     /gene="sugE"
                     /locus_tag="PC472_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2727282.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5882..6709
                     /locus_tag="PC472_001957"
     CDS             5882..6709
                     /locus_tag="PC472_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2727283.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7531..8205
                     /locus_tag="PC472_001958"
     CDS             7531..8205
                     /locus_tag="PC472_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727284.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8193..9353
                     /locus_tag="PC472_001959"
     CDS             8193..9353
                     /locus_tag="PC472_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2727285.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9570..10097
                     /locus_tag="PC472_001960"
     CDS             9570..10097
                     /locus_tag="PC472_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2727286.1"
                     /translation="MKSFANNITFSRIVFALLMLSFTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFSVTILVTVGKNIALPMYLWWCIIAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10383..11051
                     /locus_tag="PC472_001961"
     CDS             10383..11051
                     /locus_tag="PC472_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727287.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11054..13522
                     /locus_tag="PC472_001962"
     CDS             11054..13522
                     /locus_tag="PC472_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727288.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYTLSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNILDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITNSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLTC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13762..15186)
                     /locus_tag="PC472_001963"
     CDS             complement(13762..15186)
                     /locus_tag="PC472_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727289.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15205..15897)
                     /locus_tag="PC472_001964"
     CDS             complement(15205..15897)
                     /locus_tag="PC472_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727290.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15949..16728)
                     /locus_tag="PC472_001965"
     CDS             complement(15949..16728)
                     /locus_tag="PC472_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2727291.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16725..17495)
                     /locus_tag="PC472_001966"
     CDS             complement(16725..17495)
                     /locus_tag="PC472_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2727292.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17492..18196)
                     /locus_tag="PC472_001967"
     CDS             complement(17492..18196)
                     /locus_tag="PC472_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2727293.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDSNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18432..18944)
                     /locus_tag="PC472_001968"
     CDS             complement(18432..18944)
                     /locus_tag="PC472_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727294.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMDFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19270..19527
                     /locus_tag="PC472_001969"
     CDS             19270..19527
                     /locus_tag="PC472_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727295.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19670..20296
                     /locus_tag="PC472_001970"
     CDS             19670..20296
                     /locus_tag="PC472_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727296.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSSINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20542..21321)
                     /locus_tag="PC472_001971"
     CDS             complement(20542..21321)
                     /locus_tag="PC472_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2727297.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21389..21850)
                     /locus_tag="PC472_001972"
     CDS             complement(21389..21850)
                     /locus_tag="PC472_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2727298.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21826..22503)
                     /locus_tag="PC472_001973"
     CDS             complement(21826..22503)
                     /locus_tag="PC472_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2727299.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22736..23122
                     /locus_tag="PC472_001974"
     CDS             22736..23122
                     /locus_tag="PC472_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2727300.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23125..24924
                     /locus_tag="PC472_001975"
     CDS             23125..24924
                     /locus_tag="PC472_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2727301.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLVSWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25279..27153)
                     /locus_tag="PC472_001976"
     CDS             complement(25279..27153)
                     /locus_tag="PC472_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2727302.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVFPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27158..28822)
                     /locus_tag="PC472_001977"
     CDS             complement(27158..28822)
                     /locus_tag="PC472_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2727303.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28836..29657)
                     /locus_tag="PC472_001978"
     CDS             complement(28836..29657)
                     /locus_tag="PC472_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2727304.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29756..30520)
                     /locus_tag="PC472_001979"
     CDS             complement(29756..30520)
                     /locus_tag="PC472_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2727305.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30517..31266)
                     /locus_tag="PC472_001980"
     CDS             complement(30517..31266)
                     /locus_tag="PC472_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2727306.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31770..32375)
                     /locus_tag="PC472_001981"
     CDS             complement(31770..32375)
                     /locus_tag="PC472_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2727307.1"
                     /translation="MKIKENVLMLDSTCGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT        11483 a         5359 c         3901 g        11669 t
ORIGIN      
        1 atatgtaact aatatgttat aatattttaa ttaaattaaa taaatttata tttttttgaa
       61 attacttaat attttatata aaaatgaaaa agcacctcat tctgattaaa aatctttctg
      121 agatactttt taaaatatta aatactacat atagcttaca taagtttaaa atttaatcta
      181 ttataaaatt accctaattg ccaaactttt taatataaat tttccaacat cctacaaata
      241 taatcaatag atatagaatc ctaacttaca ccaatatata tttatttaaa atagtcctaa
      301 aatacctgta atagctccca ctactatcag tatacacatt atttttattg gagaaagacc
      361 tttttttaaa gcaaagagta ctaataaagt aagtaccaaa ggaattaacc caggaaatat
      421 cttatcaagt aaatccgttt gaagactaaa tttaaaatca ccactagtat aacttattgg
      481 tgtagataaa ttaataaacc tacctacaag tccccccatg actaaggtac ctagtataga
      541 agccccttct ataaccttat ttactattcc accttgaagt atactttcaa cagctttttt
      601 cccatactta tatccatttg tccacattcc aaaagaaatt gaagtcatta ttatatattg
      661 agctattaca aataatatag gtcctgcaat tgatgctcct ccctcagtta aaccaataca
      721 aacagccaac actataggag ttataactcc ttgagttaaa gtatcgccaa ttcccgctag
      781 tggacccatc aagcctgttt tgatagcatt cataccatct ccacttattt catctgctcc
      841 attagctttt tcttcttcca tagctattgt tattccatgt ataacacatc cacatatagg
      901 ttctgtgtta aaaaatgcta gatgagtctc cacttcttgt tttaactctt ccttatctgg
      961 atataatttt ttaagtactg gcaacattga atgtgaaaaa gctgttgatt gcaatctttc
     1021 ataattgtaa ttagattgtg caaagaaaaa ccatcttaac catgatttta ttacatcttt
     1081 cttgcttaac gttttcttag atacttcatt ttttatatct gacataatat tttccccctc
     1141 acatgtattt aagatgtagc actatctttt ccgtacttaa ttgaagtaat tgtaagagca
     1201 agtattactc caataacaga tattactatt atatctactt taaaataaac agccattagt
     1261 attcctaata tgaaaaaggg catagtaaac ttgtttccaa tcaattttaa attcatagca
     1321 atcccaagtg ctggcaacat agccccaatt acattaaata catgtaacac attatcattc
     1381 attacttcca acataccatc tatcgcattt ggaccaaaat aaacaactaa taatgctggt
     1441 ataaaactca ttgcaaataa cagtatctgt ggtggtacta tatttataaa tgctactcta
     1501 tcaacattcc ctttttgaac ctctctatca gcccaatgtg caaaaaaaga atttactgtc
     1561 atctttccaa tccaaagtac agttgcaaca gttcccaatg gtacagctat tgccattgca
     1621 gtacttacat ctagcttaga tgctatagct aaagcagtac ctacatatcc tgcaaaagct
     1681 gggtccccca tctgagctcc ccctgctgat ataaaaccta gatatatcat atttatacct
     1741 gctcctataa ttgctccttg aacagggtct cctaatataa ttcccgttac aaatccagcc
     1801 aatagaggtc tattccatgt aaaaaatcca acataagtca accacggact ccaagaaaga
     1861 taataaaaaa ttgctattaa tactgcttga attagtgaaa tgtgcatatt cttcctccct
     1921 attttctttt aaagatttac aagatagtct tctttataat aatgttccta catcaatttt
     1981 ctttgcatct ggtactattt gtataaagca tttaacatct tttgatatta gctctctaaa
     2041 tatttctttt tcttcatctg acacagatat atttttataa aaactttttc taccaagccc
     2101 tgctcccata cctccaacat tcaattcctt taaatctacc ccgctattta ctaaacctaa
     2161 gacagtttta gggtatttga ccaatactat taaattttca tctccattat gtgattttaa
     2221 gacttcagca cctgattcta catcatgtac ttcaacctta acagttgatg gagctgccat
     2281 ttgtaaaact tgaactataa atgggtcctt tactactaaa tcatccacga ttactatcct
     2341 ttttgctgat gtaatcttac accaagcagt aataacttgc ccatgtatta atctatcatc
     2401 aattcttgtt aatgctatat tactcataat tattcctcca gatttttaat ttattttaca
     2461 actttaaaac agttttaaca tctttgattc ctgatatccc aacttcctca cataaattct
     2521 tcaagtcttt taacttcata gtatctctgt ttaaaaataa ctctaaaagc ataggtaaat
     2581 ttacacctgt caaacattct aactcacata ccttagcaaa tttatttatt ataatagcag
     2641 atgtattaaa tggagttccg ccataaaaat caagcaatac taaaacccct tcatccttat
     2701 atttctcaat tccatcttct aactttctac taaactcatc tatgctgtca ccatggttta
     2761 atcctaaagt ttctacattt tcctgtttgc ctactattaa ttcagcactt tctaacattg
     2821 ctttagccat atttccatgt gtggctataa ttattcctgc catatgaatc atacctttct
     2881 taaccttttt tatatggatt attatttagt actctaatct aattatctat attaatcatt
     2941 atcttaccaa agaaaatatc ccttttgtat actttttcaa aataagctgg tacttcttct
     3001 aaatttattc tatgtgttac taatttttca acattcatgt cacccttttg catatgatac
     3061 aatccagcac tccattcttt tcctgggaat ggaaaggagt ttccaaacca agttcccttt
     3121 acagttaatt cacttcttac tatcttttca aattgctctc tagtcaaagc tacatcccca
     3181 tatggtacac ctgcatacaa tactgtacct ccctttttag ctagcaacag tacttgacca
     3241 catgtcagtg gagtaccagc cgattcaata acaatatctg caccatctcc atcagttaac
     3301 ctttttattt cttctacaat attcttttct tttgcattta tacaaatatc tgcccctaac
     3361 tcttttgcca agtctaactt ctcatcaaat acatccacag ctattatttt tgtagaacca
     3421 aatatttttg cccattgtat tgaaaataat cctattggac ctgtaccaag tactacaaca
     3481 gtatctccaa ctttagcttc tgacctaaaa agaccatgtc ctgctataca tactggttct
     3541 agcgcagcgg cagtctcata acttatctca tctggtattt ttattaaatt tctctctttc
     3601 acctttgtat actctgcaaa acaacctcca agttctttat ttcctattat tgctacatta
     3661 ttacatcttg aatacaagcc ttttttacat tcatcacatt caaaacatgg catagctgga
     3721 caaacagcaa ctctatctcc tattttaaaa ctccttactt ctttaccaac ctgtgccact
     3781 tctccagaaa attcatgccc taaaatttct ccaaccatat gtggacctgt ctttgaatat
     3841 tttgatatat cagaaccaca tattccagct acttttactt taattattac atcatctttt
     3901 tctaatattt ttggtacatc cacatcctcc actcttgtgt ctcttatacc atagaatcta
     3961 actgacttca ctattaacca cccctacttt ttatttgaga atatatccct attgtttata
     4021 tgttaaatat ctaaagcaac tttcatgcca atatatgtaa gcattgtatt catgcatttt
     4081 ataaaattat cattaaatgt gtgtcaaagt gtgtcaaaag tttcttatat taaaacttat
     4141 gtgtgtcaat ataaacgaca ctaacatttt atattagttc tcttattata agttttataa
     4201 aatgtacttt ttttgttatt tttataaaat tgttcggaaa tttcccttct atttgtgata
     4261 taatattaaa tataaggtat caatataaat tttcatgcga atatcagaac ttttttgaca
     4321 ctaatgcctt attcaatata ataatttaac tttttggagg ttgtttatga aaaaagtgta
     4381 tcaaggaaag actaaagatg tttttgaatt agataatgga aattacttac ttaaatttaa
     4441 agacgatgta actggtaaag atggagtatt tgacccaggc gaaaatagtg taggtctaag
     4501 tatagatgga attggtagag ctaatttaga gacatctgtt aagttctttg aaattctaaa
     4561 caatgctgga attaaaactc attatgtaag tgctaattta gaagaagcaa ctatggaagt
     4621 tctaccagca aaagtttttg gtaatggatt agaagttatt tgtcgttata gagctgttgg
     4681 tagtttctta cgtcgttatg gttcatgtgt tgaagaaggt gctaaactag attgttacgt
     4741 agaagcaact ttaaaagatg atgaccgttg tgaccctctt ataacatctg aaggtttaga
     4801 agctttaaac attatgactc aagctcaatt tgattcaatg aaaagcatga ctcaaaaaat
     4861 ttctaacata gttcgcgata ctatagctga aaaaggtctt gagctttatg atattaaatt
     4921 tgaatttgga ttctacaatg atgaagtaat cttaatagat gaaatagcat ctggaaatat
     4981 gcgtgtttat aaagatggtg taatagttga ccctatggat ttaactgctc ttttattaga
     5041 taaatagtat atacatataa tttacaaata taataaactt ataaataaga taagctatga
     5101 agtaaagact ttttaaatgt tgattatttt taaacatttt ctcgccttta atttcatagc
     5161 ttatttaaat taatatactc taattagtag ataatagctt taaccctaca attcccacca
     5221 caataaaacc tatacatgca attcttgata tactaatagg ctcatttaaa atagccatgc
     5281 ctacaatagc tgtaccgacg gtaccaattc cagtccatat tgcatatgaa gttccaagtg
     5341 gaagatgttt tagtgctaat gacaaaaaat aaaaacttac aatcattcca acaacagtaa
     5401 gtatacttgg agttaattta gaaaatccat gtgaattttt tagttgaaaa gcccaaaaca
     5461 cttcaaacaa acctgcaaca aacaaaaata accatttcat aaattcatcc ctcttttcaa
     5521 atatgtctct tatttttcca ataaaataac ctgagaaata ttataaaata tctcccaggt
     5581 ttttgtcctt ccgtgttcac aacaaaaact atgttgtggg ttttatctcg gaccagacca
     5641 atcattaata attgcggaac cctataaaac tctattaatt attctatttt atctataata
     5701 atactatcaa catatataaa aaaagtcaat atatgggttc catatataat aaaaataact
     5761 tttatatacc ttttaatatt cttattcact tctctccata cccatttggg tcaatctaag
     5821 aatatctaat ttttatttaa tatgttataa taacataaaa atattgaaag gagtaatttc
     5881 tatgaatttc tataaaattg cagtatgtca gatgattact acagaaaata aaattgaaaa
     5941 tatcaatcat gctgtagata tggtaacaga agctgccatc aatggatcta aaattgtagt
     6001 tttgccagaa atgtttaact gtccatatga aaataaatat tttcctaaat ttgctgaaga
     6061 atatccaggt gaaacaacaa caatattgag taaattagct gagaaacatg gaatttatct
     6121 agtttctggt agtattcctg agttagaaga tggaaaaatc tataatactt gctatgtatt
     6181 tgataaaaat ggagctctaa ttggaaaaca tcgtaaaatg cacttatttg acattgaagt
     6241 tacaggaaaa gttagtttta aagaatcaga tactcttaca gctggaaatg atgtcactgt
     6301 aatagatacc gaatatggaa aagttggtat agctatttgc tatgatattc gtttccctga
     6361 gttatcacgt ttaatggctc ttaaaggtgc tgaaatcgtt atcttacctg ctgcatttaa
     6421 tatgactact ggtcccgccc attgggagtt atctataaga atgcgtgctt tagataatca
     6481 gatattctat gttggtgctg cacctgctag aaatatgaat gcttcttata tagcttttgg
     6541 aaattcaaga atttcagacc cttggggaag aataattgct caagctgatg aaaaagaatg
     6601 tattatttat gctgacattg atagagattt aattcctgac ataagacaac agttaccact
     6661 tcttaagcac agaagaactg atttatatga acttaatact ttgaaataaa acagaacata
     6721 accccataaa tcgtaaatta agtgttaaaa aaatttatgg ggttctcatt aatttgattt
     6781 aaaattaatt tcataaaaat aatgctaaaa ttaatttaat taaataatac taataattac
     6841 taatgattat gctaattatc agcatttact aatttaatca ctattagtta tttcttttat
     6901 taaatatctt tactttttta cttattatgt cttctagctt tacatttgga ttatggtatt
     6961 tctttctatt tttagtcaca tctttctatt gaattacttg cttaaaacac caatgcacac
     7021 atgccagata atgttcacaa ttatgtagaa atcttgctct accttcttca atgttgatat
     7081 tatctctcct agttgatatt atctttccta aaatttcttt taattaaaaa acaacattag
     7141 ttgatatatc aagtatattt taatcaactg aaaaaaacaa taataattga tatatcaact
     7201 aattaatgta gattaattca tttttataat ttatatactt tttaattaat ctaccttatt
     7261 taattaaaat ccttcctttc tatttatcca ttttcaactt aaattataag atttcatcag
     7321 ctatattttt gcataccaat aagtattaaa tatcaaatat atattctatt aaaatttcta
     7381 ataatttcat ccttgattta tttaatatat aaaaatatac taactttaat gattaagttt
     7441 tgaaatataa ctaaaaattt gataatttat tgagattttt atactaaaat atacatacta
     7501 tacaaactta atttagaagg gagcattctt atgaacaata aaatactcgt cgtagatgat
     7561 gaggaaggta taataacact cttaaaagat tactttgaaa tgaatggtta tgaggtttac
     7621 actgctcaaa gtggaaaaga cgcattaaaa aaaatatcca aaaatccaga tatcattcta
     7681 cttgatataa acatgcctga aatagatgga attgaagttt gtaatagaat tagagattat
     7741 gtatcatgcc ctattctatt tcttacagct agaattgaaa acactgacaa gataaatggt
     7801 tttcgtgctg gtgctgatga ttatattgta aaaccttttg atttagacga acttggagct
     7861 agagtatctg cccatttaag acgtgaacaa aggaagtcaa atcaaactaa aattaaattt
     7921 tataatgatt taacaataga ttacgaaaaa aggttaattt gtatatataa taatcagatt
     7981 tcactttcaa aaaaggaatt tgatatagta gagcttctct ctatgaatcc tggacaagta
     8041 tttgataaag aaaaaatata tgatagagtc tggggataca atagcaatgg aaatagtgat
     8101 gttataatgg aacacattag aaaaatacga atcaaactat caaaacatac tcttgaaaat
     8161 tatatagaaa cagtctgggg agttggttat aaatggataa aataaataat atgtcattaa
     8221 aaaaagcttt ctttgttctt actttatctt cactaattat agcatcagta cttactactt
     8281 tcgcatacat tttacttaat aatctatata attctataca agataagtat ttaaaaacac
     8341 ctataaatac aggtattata accatagttc aaaacaatgt gaaccaattt agtgattatg
     8401 ataaaacatt acttaatata atcaacatac tccagcttgt acttccactt atatttttta
     8461 taggactttt attattagct gatataattt tttacaaggt aaaattaaaa acacccatag
     8521 aaatactcaa taaaggtgca atggaaatat ccaacaacaa tttagacttt tgcttagaat
     8581 ataataataa tgatgaatta ggtaatctat gtaatgcttt tgaaaaaatg aggtctgaac
     8641 taaataaaaa caacataaag atgtggacta tgattaatga tagaaaacaa cttaatgctg
     8701 ctttttctca tgatttaaga aatcccctaa cagttctaaa aggttactca aactatttaa
     8761 caaaatacat accaacaggt aagcttagtg atgagaagat tttatcaacc acacaactta
     8821 tgtctgagca tattaacaga attgaatact atgtagaaaa tatgagtaat gctcaacgat
     8881 tagaagattt agttgtttca aaatctatgt cgaatataaa caagtttata gaaaacttag
     8941 atgaaaatat atctatcatt gctaaacaag aaggaaaatc ttttacactt aaaaatcaaa
     9001 taaataatat aaatctattt tttgatgaaa atatcattct tagagtcgtc gaaaatataa
     9061 tatcgaatgc ttttagatat gctagaaata atgtatccat acttatatat cttgaacaag
     9121 aactccttac ctttgtaatt gaagatgatg gtataggatt tagtaaggaa tctctaaaat
     9181 tagctttaag acctttctat agagataaga ctttaaatga cagcaatgca cactttggta
     9241 tgggtcttta tatctcaaaa atattatgtg aaaaacatgg tggctctatt tctattgaaa
     9301 ataattctac taacagtgct aaaattactg ctaaattctc aacaagaaat taaaatgatt
     9361 ttgtaagctt tcactgctat tatataaaat ttgttagttt agaatttata aaatgtattt
     9421 caatttataa ccaatatata tactattttg tagaaattgt aagagttgct tcaattataa
     9481 tattttggca aaaggcattt gaaacaataa tagaaatata tatttaaata tgtagaatag
     9541 tatgaaatta gaattttgga ggtgattata ttaaaagttt tgctaataat attacattct
     9601 ctcgtattgt gtttgcattg ctaatgctta gttttacatt ctgttcactg cctttctgga
     9661 tatgttattt ttggtgtgga attagtgata tacttgatgg tcttattgcc cgaaagttga
     9721 gacaacaaag tggaataggt gcaaagttag atagtattgc tgatttcatt ttttcggtta
     9781 caattttggt tactgtcgga aaaaatattg ctcttcccat gtatctatgg tggtgtatta
     9841 ttgcaatagc attgttacgg ttgattagtt acagtattgg tttttttaag tatcgcacat
     9901 tttcatcgct tcatactttg atgaataaaa tcactggaat ttctatcttt gcgtttccat
     9961 tactctatat ttgctttggt cttgatgttg caggaattat gatatgtatc gtggcttttc
    10021 tttcttcttt tgaagaaata gcaattatga taaaatcaaa agaattaaat cgtgatataa
    10081 aaagtatatt tatgtgaaaa cttagaattt gtgacaaaaa taatttatat aataatgagg
    10141 aagtaaaaca taaaaaatta tccacaagtt tatgtgtaaa atagttttaa agcaatagag
    10201 ttgtattgta aagcctttag tgcataaatt acattttaaa ttaaaaatga tactgaaact
    10261 gcttatcagc actgcgaatt atccgcaaat agtgaagact ttttagattt aagctaaaag
    10321 ttgggaaaaa attgagtttt aaaacttaca ctcttcttat atagaaaagg agagtgtttt
    10381 ttatgattat aaaagcaaaa caattatcaa aaatatatgg ttctaataac aataaagtta
    10441 tcgcacttaa taatgtaaat ttagaaataa actcaggtga atttgtttct gtaattggac
    10501 cttctggaag tggaaaaagc acacttttac atattttaag tggattagat aatcctacct
    10561 ctggacaagt gttacttgat gataaagata tatataaaca cactgaaaaa gaactatctg
    10621 cacttagaag aaaatccttt ggattcgtct ttcaacaatt taatctttta cctgttctta
    10681 cagcatctga aaatatttca atgcctgtac ttctagataa aaaacaacct gataaaggct
    10741 atttaaatga aatttcatca ttacttggaa ttgcagatag actcaatcat cttccccatg
    10801 aactttcagg cggtcagcaa caaagagttg caattgctag agcacttatt gcaaaaccag
    10861 atataatatt tgctgatgag cctacaggaa acttggacag taaaagtggc agtgaagtta
    10921 tgaatcttct tataaaaact tcaaaacagt ttggaaagac tttagtggta attactcatg
    10981 atgatagaat tgcaaaactt gctgatagaa aaatttcaat tattgatggt gttcttatgg
    11041 aggtgaaata gaaatgaaga attacctttc actttcaata aaagaattaa aaagccagaa
    11101 acttatgact acattcatca ttatagcaat agtactatca actattatga caactgtggt
    11161 tggacagtca attggtatcc tacaaaattt aagaattgaa caagcccgtt cttttaatgg
    11221 tgatagacat gtatcctttc accaactcac taaaaatcaa gttgatgaca taaagaaaga
    11281 tgatagagtt tatcaagctg gtacttcaat aactattggc agtagtaaaa taaaggattc
    11341 tggtatctct gtacttgtaa aagagtatga taaaacagga ctttctaact acccaaaatt
    11401 aatgaaatta aaatctggac atttgcctaa agataaaaac gaaatagcct tagatgaaaa
    11461 cacactaaag cttatgggta taaaaccaag actaggagtg actataccta tgaatttaga
    11521 tatttcactt ttaaatgata ccataccacc atataattac acagctaact ttaaattgag
    11581 tggcatttta gaggatgatt atacaggata tgtaagtggt atagtaaatg gtattgtagg
    11641 taaaggtaca tcagaaaatc tattacctaa gagatatact ttatcctctt tggattttaa
    11701 aataaaacag caagaaaagt ttcaagaaat agtaaatcaa ttagctaaaa aaataaatct
    11761 ttctcacaat tcaattcaat ataattggat ttacttaaat gctcttaaga tacagtttga
    11821 aaaagatgaa aatagttcta atagtgatgg tatatctatg attatattgg tatctttatt
    11881 tgttgcctta ttggttttac tggcttctgg acttgtaatc tataatattt taaaaatctc
    11941 tgttacaaag aaaattaaag agtatggatg tttaagagca ataggagctg aaccaaacca
    12001 gatatataag atagttattt tacaaatctt aattttatgt actgttgcca tacctattgg
    12061 agctgtaatt ggaattatat cttcgaaagg cataactgga atggtcacta atatcttaaa
    12121 tccagatata cttctagcaa atgataataa agaaattact gagcttatac ataaaaatac
    12181 aacagcatat atgttcccat tagtattaag cactaatgta tctttgattt tttcacttat
    12241 ttcagcatta ccatcagcaa tatatgcttc acatgtctct cccaaaattg ctatggctgg
    12301 tagtactacc aaaatcaaaa gaaaaattaa aagagaaaaa acaataaaaa actttgaaag
    12361 acatctagca tggctaaact taaaaagaaa taaaggaaga actataataa ctatactatc
    12421 attatttatg agtataacag tattcgttgc actgagtgag ttctctaata tattagatgt
    12481 atctaggtca gtaagtaatt taaaagaagg agatttttcc ttaacaaatg aaatatctgg
    12541 ttttgataaa tcaacactag ataaaatagc taaaatgaaa aacgtcaatc gcacttcctt
    12601 tataaagtac tctgaataca aacagggtga aatagataca gatataaatt ttgaaaatag
    12661 tggtgagatg ttaaaaatca ttggaattga tgagcaaact ttaaaagact taatgccttc
    12721 tattacaaat tctatactag aagactttaa aaatggttct atctgtttta taaaaaatcc
    12781 gattgctatt tctacaccag gtgtaaaaac tagatataca aatttaaaac ctaaagatag
    12841 tatcacaatc aacaaaaaac aactagatat atatagtaca gtagataaaa tgttcttttt
    12901 acaaggcaat ggctggataa atggtgttga tgttattgta tatgattctg tttataacac
    12961 tttgacaaat aaaaacaagt tcaaccagat aaatatttat gctaaagata aatcaaaatt
    13021 agaacaaata agattgtcta ttgaacagat ttgtgaaaat aatcctggct ctcattggat
    13081 atcatatatt gaatcagata aacaacttaa agagagtttt aaacaaatag aatttctagc
    13141 atgggcagtt atattatttg tgggattgat tggtacttta aacatcatta acacaactca
    13201 tacaaatata aatactagga ctaatgagat tggagttaaa agagctattg gtatgagtaa
    13261 taggagctta tataaaatgt ttttatggga aggtgtttat tatggaattt ttgctgctat
    13321 atttggttca attgctgggt atgccagtgc tattattata aacatggcaa ctatagaaaa
    13381 gcttgatttc actaatatac caattacttc aattttacaa gctactatta tttctgtact
    13441 tacatgtatt atagctactc ttattccgtt aagaaaagtt aaaaagatga atataattga
    13501 ctgtatagat atcaatttat aactatttta cagataataa tataaattaa attatcaatg
    13561 actgtctcta ttttctttca aatctaacta ataaatcttt ttaactataa tataagcttt
    13621 aaaaaaggcc tgtctcaaaa tagagataag ccttttttca ttgtcgaaat aatgaaaata
    13681 tatttaactt cacaaaaaaa gagtgcctca cgaattaaaa atttcgcttt gagacactct
    13741 cctttaattt aaaatatata gttaaatgct atgccaaaat ttcacacatg ctccacctga
    13801 ttttgaattt tctattttaa cagaacctcc aagctgttcc actaattgtt tgacaatgta
    13861 aagcccaaga ccagaatgtc caccttttgt ctgcctagct tcatctcctc gataaaattt
    13921 atcaagagcc ttttttaaat ctttagagct aaaccctcta ccagaatcac aaatctcata
    13981 agaaatacaa tttttttcat cctttacagt aatactaata ttaccacctg atggagtgta
    14041 ttgcagactg ttggatatga tattatcaaa aattcgtgtc aacctatcca cgtcgatttg
    14101 aattggggat tgtatattcc cctgcatttt caatataagt tcaacttcct tttgatgagc
    14161 ctgtaattca tagtcttgca ccttttgcct caaaaattca ggtaaattta taggaacaag
    14221 atttaattgt atatttgact tttctaagtc agacgtatat tgcatctttt ggacaagagc
    14281 agcacttttt tctgtatttt ctcttatcac tgtaagatac ctatgaagtt tttcattgtc
    14341 atctgtatta ttaccaataa gtgcatctgt atatcccaaa ataatagaaa gaggagattt
    14401 taaatcatgt gctagagctt ccaccatttc aacacgttcc tgctccatct tccactgtgc
    14461 agataaagag ccttttaatt cgtctttcat ttcagaaaat gcactacaaa gtttaccgag
    14521 ttcattatct gaataatagt cgatttcaaa atcaaggtcc ttttctttta tcttcttgga
    14581 cgcatcaatc aatagatgta atggttgatt gatattcttt acaaagcgct tagaaagcca
    14641 tctagtaaaa cccacgatat atagaaatgg agaaaacaaa gccattataa taactgcaaa
    14701 tacaaatctc cctctaatat ttgcaaaggt gattttcact tggtagaata gaataacagc
    14761 accctcaatc tttccattat cccctttgat tggaacagta tggatataac cacctttacg
    14821 aacagtttta ttgataaaat tattaaataa ttcttctttt gttttaaata gttttttggg
    14881 gttagtacca tataaaatat tgccattatt atccacaact tgatatagca tatcatcccc
    14941 acgaatcgtt ctttttaaac cttcttcatt cgattgtgat aacaaagcta tgtttttttc
    15001 attgatgtat ttttcaattc taggaacttg tcgctcataa tagtttggtg gatagatatc
    15061 cttatttaag gaatgaatat acaaatagag agcaaataca taagtaatca ctgtggcaac
    15121 tatactggct attataataa aaataaaagt atgtctaaat tgggtaataa gggattgatt
    15181 tttcatttaa taacctctct ctctttacat tttattccac ttatacccaa taccccaaac
    15241 tgtggaaata tattcttttt ctggtgtcac agcagaaaac ttagctcgta ttttttttac
    15301 tcgttcaaca acagttgagg aatcaccttc agaatcacac ccccatactt tttcatagat
    15361 ttgttctctt gagaaaactt gtcctgcatg gagtgctagt aattcaacaa tatcatactc
    15421 tatttttgtt aaatcaatat tcttaccatc aattttaatt gcacgttcct tcatatataa
    15481 gcataacttc ccaaaataca aagtagatct cttgttctct tcattgatat actgtgaacg
    15541 cttttcccta cgcagattag cttcaatcct tgccataagt tcacgcagtc caaatggctt
    15601 tgttacataa tcatcccctc caaaggtcaa ccctttgatt ttgtcggttt ctgattgctt
    15661 tgcacttaaa aatataattg ggcataaaac atcatctcta attgcctgac atacctcaaa
    15721 tccattcatt ttaggcatca taatatccag tataataagg tcaggctcct gttttgataa
    15781 ctctatacct gcctgaccat cataagctat caacacttca tgtccttttg ccttcaattc
    15841 atcctcaagt agcattacta ggtccatttc atcatcaata atcaatattt tactcaatat
    15901 aacaccacct ttctcttatg ggaaggtcag tattaaaaca atattcgtct actcataact
    15961 tttccttccc tcccacattt taaaccaaat aacgcctcct atcaagaata taatcaaacc
    16021 tattgctact actataagct ctatactagc tgtttgcatc actttagagt aaaatgcctc
    16081 agttgaaata gttccagctt ctttaataaa ataaattcct ggaagcatac ctagttttac
    16141 tggccatgtc catggaacaa atgcccatac tttttcacca acgctagtta atccaagtat
    16201 tgctgccatc aaaattccac caatactaat accaatagat gcacccatac cccatgcaaa
    16261 actcacccaa agatgaatag ctaatagtgg aagtgtccca atcaccacaa gtcctgctgc
    16321 tgacaggaat aaccatattg ttgctccaga tggcactgca atactcattc ctatgcaaag
    16381 tatcaacgta gcaataaatg tgcatatagt taggcaaaac agaagaaaaa gaaattttcc
    16441 cagatataat ctgacacgag aaatacctac acataaaaaa ccattaaaat ttccagcaag
    16501 ctcttcctcc tgcactataa atcctgctaa aatacctact ccaataggaa tgatgaatac
    16561 agtccatact gtaaaaaatc cttcaaaagc aaattcttgt gtacttccag atttagttgt
    16621 gagataaaaa accacacata atgaaaaaat aataggcata caaagagtaa gccactggac
    16681 agctgtcctc tttgctcgaa gccattctga tgataatagt tttatcattt gtcatccctc
    16741 ctattgaacc aagctgcagt aagtgctgtt aatactataa acacaaccaa agaaacaaca
    16801 atcccaatag gaatcactga agtattcaaa agtgggtctc cagcttctaa tatagtaccg
    16861 ttaggatgta tgttgatgat tgggcatacc attctaactg cccaactcca aggtactgca
    16921 atccaaaatg gttttgtcgc agcaagaaca ccaaatatca tacctaaagc tccaactccc
    16981 atacttagga acataccttt ccatgtagct agccatagct gaagtggaag tatagcaaga
    17041 gaaacaaccc agcatacaat acttgcccct ataatctgtc caaaaggaac tggaccagct
    17101 ttcaatatta aaccagttat aattgttacg aatattaaaa ctagcgttga aataaaactg
    17161 tagactgcca tagctataac tttattaatc caaagtgtca taggagacac attgtgtgta
    17221 cgtaaagcac gataatttcc agcttttttt tcctggaaag ctaccaaagt agcaaacaat
    17281 gcaaatccaa taggcagaaa taaaaatgac caccagttaa aaattaatgc taaaagattt
    17341 tgccacgccc aagaagttgt ttgagataat ggattttgca tgagtgttga ttgcattata
    17401 aatgcatatg ctgcaaaaga cactgggaaa aaaacaataa gtttacccat aaatgttctt
    17461 ttatacttta aaagttctga ttgtaaagca ttcatctgtt ttgtcctcct ttaagaataa
    17521 catctgtaaa aaattcttct agattttcat tagagtcagg cattccttga aataatagtc
    17581 gtcctccatt tataattcct atattatcta caacttgagc aacctctgat agaatatggc
    17641 tagaaagaat tacagttata cctttttttg gaaaagaagc aatcaattca cgcaattctt
    17701 gaatgccaaa ggggtcaaga ccattggttg gttcatctag tattaacaac tctgggtcac
    17761 tcaaaagtgc aatagctatt cctaaccttt gtttcatacc catagaaaat tgtgaagctc
    17821 tctttttacc tgtatttttc aaatttacta tttccaacac ttcataaatt ttatctttag
    17881 gtattcccaa tatttttgta tgtactaaca aattttcctc tgctgtaagg tttccataca
    17941 atgcagggga ttctattaaa gaacctattt ttgcaagaga ttctcgtttc caaggctttc
    18001 tatcaaaaat aatacgacca tttgttggac gaagaagacc aacaagcatt ttaagtgttg
    18061 tggactttcc tgctccattt ggtcctaaaa gtccatatat actatttcgt tcaatttgta
    18121 gagaaacatt gttaactgcc atttgttctc catatctttt tgataagttt tttgtttcaa
    18181 gaattaaatc tttcatatta atttctccct tcttgtaata ccttaaatct gttactaaga
    18241 gaatactaac atgaaaaaat aacaattcta taacacctac taattttaat aaaatttaaa
    18301 ataaaaaata gagttaccaa ttttattttg gcaactctat ttaagtttat gttttacagt
    18361 tatattagat ttaaacttag tttcattatc acactaactt caaatttact ctataatctt
    18421 taaatcaaac actaatctat taatttatat ttctttagac atctaatata gtttgctact
    18481 aaataataat tatttatatc atatttatct aatcctttaa aagaagttat ttcatcatta
    18541 agctcatcac taccttctac aaaaacacaa cctgtttcaa agtccatacc tacaataatc
    18601 ttacagccac tataaatttc ctcttgagta tcgtacaatt tttttgcatt tccttcattt
    18661 ggaattgtaa atacaatagg ttttaattct ttatcactgg aaatcttttg tgaaaaaggc
    18721 ttgtttaact taatattatt gataactaca tgtttaaatt cctctgaagt ttcttctact
    18781 attacatatt ttcgctttaa aaactctatt atttctaaaa cagacttaga atttactttt
    18841 aaaatacttt tatatttttc atataaagat ttccactgtt caattttttc ctgtggtact
    18901 tcttggatat gtgtacttat tttttcttga gatatttttt tcaattaaac tctcctttca
    18961 atacttaaat atccttataa atctcacata actaaagtta caaggttcct acttaataga
    19021 attttgcata cttaaatatg tcttattaat tctctacagg atatctccgt agttcctacg
    19081 attcttacat aagttactta ttttaaattg ttattagtta tattctaaaa attatttatt
    19141 ttatcactaa tataatactc ttaaaaactt tacaaatcaa tttaaatttg ctacctttta
    19201 tttataacaa cttatagtac atttctccta tgcttttcaa tatattactt ttaaaaggag
    19261 gttttcatta tgattttaaa tagagctgag cttttccgag gatattcttt aaatagattt
    19321 aacgagatat gcaatatatt atcagtaaat aaaattaaat ataagtataa agtgaaaaat
    19381 aatgcttact ctaaaaaacc tttttttaat gaaataacac ttggcaattt aggtcaaaag
    19441 gaagatttct cttatgagta cttaatttat gttcataaag acgattatga acaggcagag
    19501 tctctaataa attctaagtt gcgatagatt atttataaaa catgacattc tgttgtcatg
    19561 ttttatatgt tattatactt tcaaatatgc tactatattt tcaaaattgt gtgctttaaa
    19621 actagctttt tatagtaatt taaagcttaa aactaaggag actcgttata tgaaaataaa
    19681 tataaaatca aagttaagtg aatttattaa acagaacaat ctttttgatg acagcagaat
    19741 aatatcctac cttcctaata taacaataga aacagacaaa ataaaaacta taatgattaa
    19801 tgaggttgtc ccaactaaca ccaatgacga tttttatagt gtagataaag atgctgatta
    19861 tttaaaaact actattcctt tatttgacag tgcaggaata gaagtatcta gcataaatga
    19921 tatcctagat atgggtatct atataactaa tgcagtaaaa cttcctaaat cagaatatac
    19981 aataactcgt gatacaatta aattacatat gcctatttta gaagaagaaa ttaaattatt
    20041 taaaaaccta gaggtagtaa tgttaatggg agatgttgca aaaaaatctt ttaatatgat
    20101 tactaaaaag catataaaaa agaatgtcat cccttccatt tcaacatata aacttcgtaa
    20161 taatgaactt tattatgata atttaagagt atttccatca tatattatga ctggtggaaa
    20221 catactgatt gaaaaatcaa aatttgaaat ggcatcagaa gatataaaaa aaatgttcga
    20281 gattataaac ggttgatatt acaaaataat aaaaactaat ataaaataaa agtgcatcaa
    20341 aaacttctat ttatgaggca cttttatttt atattagtgt aactatatac tattaacata
    20401 atttattatt tatattgttc tttttaatct ctattccatt tagtctaggt aacattactc
    20461 tttacaagat tactttatat ttcttttata tctctaacgt ttttatttaa taaaacaatt
    20521 attaatacaa gtcaaatata tctatttatc taaaaatata tcttttacag taatatctgg
    20581 atggtgatac ttcttcctat tcgttaaaaa agattttttt acattaatag cctcttttgg
    20641 acaccagtga aaacaagcta ggcatctttc acactcatta ccccactttg gctttttatc
    20701 aactatactt atattctcta caggacaaac ccttttacat gtcccacatc cattacattt
    20761 attttctgca aaaaatttag tagttgcaat ttgcatattc ttacctacta aacgaagtac
    20821 aacactatct atatgtgact ttaaattgtt ttttccttct atatttccaa tctccaagtt
    20881 attgatacat tttgcaatct cagctaccct attagtctga tttttcaatc tttcttcttc
    20941 aacatctgga ggtgttatca aagcatttcc aggcatattg attaaaaaac cagcagataa
    21001 ttttttcccc ctttttttaa gtaacttatc tatagtaaat aaactatgtc cagctacccc
    21061 attacaagtg gcaatggcat atatatgagt attaatatta aaatttaatt tctctacaaa
    21121 agactttact atatcaggaa catcagcaaa ataaacagga aaaacaatta ccaatatatc
    21181 ttcatttatg tctatattat tttcttttct atgtatagct aagggtataa cttctttact
    21241 tccatttaac gtttctttta aaatatttga aactactagc gaatttcctg ttccagaaaa
    21301 ataataaatc ttactattca ttcttatccc tctttctata tttctacatc atcaatagtt
    21361 aaaaagtctc ttaaaatagg atttatattt actttccaat tctatccaaa atatcagtaa
    21421 acaaaggcaa ttcttcatca gagatagatt taaatgtctc tgttgcatat atacttgcaa
    21481 tttcatttaa tggttcttct aattctctac ctaaatctgt taggaatatt tgaaacttac
    21541 gtctatcttt aacatctact ttacggacaa ttaatccctt atttttcata acatctaaga
    21601 ttcttgtcgt attagattta tccttacatg atttttcaca taaatctatc tgagaaattc
    21661 catcttgctc ccatagaaac cttaataacg cataatggtc agttgttata tcatatccat
    21721 tttgtttaaa catctttttt aaatcatctt ttacttttaa cgaagtactt actaacttag
    21781 ttgtagcttc atatagtttc tcaaaatctt tcattttacc tccatttaaa taatcccttg
    21841 ctcctcccat ctatcaatta ttcccttata ttgctcaata tctttaaaag ccattggctt
    21901 taaaacagca tttatagcaa taaaattata gatttgagaa agcacaggtg gatttttctt
    21961 ttcctctatg caataaataa attttttaga taccttttgt atctttgatt ctactttttc
    22021 agttatagga ttttgattaa atccagtttt tatagataaa ctaccacaaa cataacctcc
    22081 cgaatctaag accatggatt ttataaaaga aattgctttt gaaccacgtg cattttcata
    22141 tgtagtaact gctatacatg gcttacgata caataatctt tcaagcatca tatgtgctct
    22201 atctgtaaac accttgaaaa gtccagttac actacttcca taagttgggc taccaaatat
    22261 aacaccatca ctcttagtaa ttatatcatg aatatactct actttatcat tttgattgat
    22321 acacttgccc attttataac aatttaaaca accaatacaa tatgatggat tgacctcact
    22381 taaatcaaaa taattaattt ttacatctgg tattaatctt tctatatctt tatacaagta
    22441 agtcaaaact tttgatgtag ctccattttt tctagggctt ccatttataa ctgtaattat
    22501 catttctata tctccttttt agttgttata tcaacaataa gtatatcaac tatattttta
    22561 gctgtcaata taaaccattt atttaaagct atacttacac agcttaaata aaagttttat
    22621 ttaactacat gtgcttattt aatcaactat aaatgaatta tttttaataa ttgacaaact
    22681 aaattaatga taatatactt tatgtattac aaatgtaagt agaaggaggt atagtatgaa
    22741 aaagttacca catatttcag aagcagaata tcaagttatg aaaattattt ggaaatatgc
    22801 tcctatcagc actactgaag taattgaaaa gttggtagaa acaagtacat ggagtcctaa
    22861 aacaattcaa acaatgttat tgagattagt taaaaaagga gctttaacat atgaaaaaaa
    22921 tagcagagta ttcgtttata ctccacttgt taaagaagaa gaatacgtag ctacagaaag
    22981 tagttctttt ttaaatcgtt tttataatgg tactcttaat tctatggtac taaacttttt
    23041 agaaaatgat aaactatctg aagatgatat tgaggaactg agagaaatct taaataaaag
    23101 gacgactaag ggggataaat aaatatgtat gccccattct ttatacgatt tttactcagt
    23161 acattaacct tatcaatatt aattgttgta attttacttg ccaaaaaagt atttaaaaaa
    23221 catatttctg caaagtacca atacaatatt ggatttatat tgcttataat gttaataatt
    23281 ccatttgtcc ctcttaaata tattaatctt ggaaatgtat ttaactattt atatactttt
    23341 aatcaacttg gaagcagtac taataggact tctatgataa aaaatagtcc aaattctata
    23401 attaataact caaatgcatt aaacgatttt tcagtatccc taaaccaagt taattttgat
    23461 agtttaaata ttttattact agtatcatgg ataattggaa ttttaatcat gatagctatt
    23521 actattcatt gcaacattaa aataaaaaat atgaaaaaat caatacaaat tttaagagat
    23581 gatgagatag tatttatatt tgaaaagtgt aaaaaagata ttggtataaa taaggatttt
    23641 atactatgca aatcacctat tataaataca cctattacat ttggatactt taaaccacac
    23701 attatattac cagataagtc tatatctaaa ttatctttaa aagatataaa atatatctta
    23761 cttcatgaat tacaacattt taaaaataaa gatatactga taaattatat aatgtgcttt
    23821 cttcaaatat tatattggtt taatcctctt gtatggtatg catttaaaga aatgcgtata
    23881 gaccgtgaaa ttgcatgtga tatttcagtt ttaaaaaagc ttgataaaga tagtcatata
    23941 gaatatggac aaactctaat aaactttgtg gataatattt caagaccatc aaatcttaca
    24001 cttacatcag atattggtgg gtcaaaaaaa caaataaaaa agcgactatt gaagataatg
    24061 gattttcata atgaatcaaa tacgttaaaa ttcaaaagca ttttaatttt tacattgata
    24121 gctttaattg tatttggaaa tattcccaaa ctatctatta tggcagatat caacgaaaaa
    24181 tatagaccta caaatgaaca aactgtgtac gaggacttaa gtcagtattt taaaggtttt
    24241 gatggaagtt ttgttatgta tgatttaaaa tccaagcaat atcatattta caataaagaa
    24301 aaaagtaaaa caagaatttc tccaaattct acttataaga tttatagtgc cttattggga
    24361 ttggaaaatg ggataataac aagaaataat tcacatatcc cttgggatgg aaagcatcat
    24421 tctaatagtt cttggaatga agaccaagac ttattttctg ctatggaaaa ctccgtaaac
    24481 tggtactttc agactctaga taaaaaaaca ggtctaaaaa acatacaaac ttatcttagg
    24541 caaattggat atggcaatta tgatgtttca ggtggtatct caaacttttg gctagaatct
    24601 tcattaaaaa tatcacctat tgaacaggta gaattacttg aaagttttta tacaaataag
    24661 tttaaatttg aagataaaaa tatagaaaca gtaaaagatt cattgttaat atcaaaaaag
    24721 gatggggtat cactttttgg taaaacaggc actgccaata taaatggaaa agatgtaaat
    24781 ggttggttta taggttatgt ggaaaaaaat gaaaatactt atttctttgc tacaaatatt
    24841 caaagcaaag attattctaa tggtagttca gcatctaaga taacattatc catattaaat
    24901 gacaaaaaaa tctataatcc ttaatatatt tactgtacat tctaaaaaat tcacataatt
    24961 ttcatattaa ttatcaatta aattcatata caaggcttat tatataaaca tagaagatag
    25021 taaaactagc ttaaaaacat aatataatac cctatacaaa tagtggcaac tctccctcac
    25081 atcctcaatt gttgccacta tttaattttt tgtatgaagt taaatatatt ttcatcattt
    25141 caacaatttg ggtgcaatac aatctctatt ttgaaacaac ctctttaatt gttttagagt
    25201 ttatgaatta tttctgatac tttaaaaaga ttgaattact cttgataatc ttcttttgta
    25261 gtcattacat atcacctact actcaacaca tattctttga cctattttta catccttgtt
    25321 tatatccaaa atatttacct tcttaccatc tggaaacaat accattgtca aagtagactt
    25381 tcctaattcg tgtattctct ttaaatcaac attaacaaga acatctccct gtttaaccat
    25441 atctccttgt ttaacagcaa catcaaagcc atctccattt aatgcaactg tatcaattcc
    25501 aacatgaatt aatacttctc cattagttcc tttaatccca aatgcatgtc cacttgaaaa
    25561 tactgtttct aatttacctg aaataggaga aacaattttt ccatctataa tatcaatagc
    25621 aaaaccatca cctaacattt ttttagaaaa tgtttcatct ggtacatttt ctaatgcaac
    25681 aattttcccc ttagctggaa acacaaagtt ttcattattt tcattaaaca ctaattcttc
    25741 gtcttcatct tcatttatag cttctttagg cactccaaat aaacatgtta caataaagca
    25801 gaatgcaacc acaaatattg atactaataa gtatgtgatt aaattatatc catcatagat
    25861 atacattaaa taagaaggta aaactgctaa tccatatgaa ttagattgaa tatgtaaaat
    25921 tgataatatt gctgctccta aacctgaaga tgcacacata ataatcaaag gctttaaatt
    25981 atatcgaagc tgaatcccaa ataaaacagg ctcacttata ccaaaaagtt gagatagcat
    26041 tgctccaacc gcagtttgtt taacttgatt atttttagat tttttcataa atgctagaca
    26101 tgttccaata ttcgcaaatc catacattgc acaaagagta ataagggcat taaatcctgt
    26161 atttgctaat agtgatgttt caatcattac ataagtatga tgtaagccag taataacagc
    26221 taatggatat gtaaatccaa taataaatcc tccaattcct aaaggcaaat caatcaataa
    26281 gctgatgata tcaaccattt ttaattctat tgtatgcatt atagggccta cacctaacaa
    26341 aataactaac attgttatta gcataacaaa aaatggtgtg aaaataaggt ctaatacatt
    26401 aggcatatgt ttacggaacc atttttcaag attagctcca ataaatgctg taacaatagc
    26461 tgttaaaaca cttccttgac aacctacaat aggaataaat ccaaatgcca taattgcttc
    26521 aatttcattt gatgggtcag caacagaata tgcattagga agtattgggg aaactaacat
    26581 aagtccaata acaagtccaa taacaggtgt tcctccaaat actttaaatg ctgaccatac
    26641 aataattgct ggtaaaaaag caaatgctgt ttctgtaaga accgaaacaa gtgtctgaat
    26701 atacaatgga atatttgcag aagacattcc aaataatcca agtacattat cattaaacaa
    26761 acaacccttt aatcccaaga ataatcctgt tgctgcaata actggaataa taggaacaaa
    26821 tatatctgct agagttctca taactttctt aaatccagtt tcattatttt taactaattc
    26881 atcttgttct ttttttgata aagtttttaa tcccattttt tcaacttctg cataaacttt
    26941 attaactatt cctgttccta aaataatttg atactgtcca gaagtaaaga aaaccccttt
    27001 aacctcatca actttttcaa ctttcttatc attaataatt tctctatctt taaccatcac
    27061 tcttaatctt gtggcacaat gtgtaataga aataatatta tcttcaccaa taattttaat
    27121 aagctcattt gctattttat tatatttatt catttttcta cctcactttc aataatactg
    27181 catgaaatgg tttcaaacgc actgttttat ttaatagatt tatatcatct aaattttgga
    27241 atacaatatc atatttttct tctactctta tttctacagg tttatcagaa aaattataat
    27301 aacaatcaat aactaccaaa tctaattgcc ttctatacgc aataatatca tcactttcta
    27361 tatcaatagg ttctattgaa ccataaatca aacaatcaga atactcacta tcttgtctca
    27421 actttatcat ctttttataa aattctaaaa tactctctga attgttaatc tgactctgtg
    27481 cattaatatc tttataattt tcaataacct ttaaccatgg tgttgtatct gaaaaacctg
    27541 cattttcttt atctgcccat ggaaatggcg ttcttgcatt atctcggctt cttaaattca
    27601 caaattctaa cgcttcttct tctgagtaac tttcatctat tgaacgatag tattggtcaa
    27661 tgctagataa atcattaaaa tcatttatgg tatctctttt aaaatttgtc attcctaatt
    27721 cctgtccctg ataaataaat ggggttcctc ttaaaaagaa atacattgct cccaacattt
    27781 ttactgcatc atcattcctt tcgtattgca aaagatactt tgttgtagct cttggttggt
    27841 catgattttc aataaaatta gctgcccatc catgttcctg aacatacatt tgactatcta
    27901 aaatgcattt tcttaaatct ggaattgtcc atgaaattct tttaaaccac tcactaccag
    27961 aagcaacatc taagtcagca tgtttaaagt caaagaccat tgaaaagaaa ccatcttctc
    28021 caataaaatc acctagttgg tcatagctaa tacctgctgc ttcagcaaca gtcatacaat
    28081 catatttttt aaacgtattt tcttttaatt cataaagcat tttttctatt ccaggctgat
    28141 ttctaactgt ttttgtacat ttaacaagac catcaacacc atcactttct aaactttgaa
    28201 aacttaaatt ctttttgata aatgtaatag catcaactct aaatcctgcc actcccattt
    28261 gagaccaggt attaatcatt tggtaaagtt cacttcttaa tattggattt tcccaattaa
    28321 ggtcaggttg ttttttacca aaagaatgaa aataatattc gtctctattc tctacctttt
    28381 cccaaacact tccaccaaaa acagacctcc agttatttgg taaatcgttt ccttttttaa
    28441 aaatataata atcatgataa ggactttctg gattttttaa tgcttcttga aaccaaggat
    28501 gttcatcact tgtatgatta agaactaaat ctaatataat tttgattcct ctatctttac
    28561 tttctttaat taactctttt aaatcttcca tacttccaaa ttcacttgca atatccaaat
    28621 aatcactaat atcataccca ttatcgtcca taggtgattt aaaaattgga cataaccaca
    28681 atgatgtaat acctaaatct tttaaataat ctaaacgaga aataattccc tgtaaatctc
    28741 caattccatc attattacta tcttgaaagc ttcttggata aatttgataa acgatttctt
    28801 tttgccacca tttcttttcc ataaattact cctttttata tgatatttct tctttttaat
    28861 tctttataaa ttccattatc gaatatgtcc tcacatatag atgtcgctat atctttaagt
    28921 tgttgatgac tatttttcat tgcaattgta acgtctgatg cttgaaacat gacaatgtca
    28981 ttttgaccat caccaaaaca tatggtttct ttttgagtta ctcctaatct ttcttgtaat
    29041 ctcttaattg ctttgccctt atgaaaatct ttttgaataa tttcataata ctgactagaa
    29101 atatctctct gtgctaattc cattttatct tgtagtatat ctttcacttc gtcaaatacc
    29161 ttttcatttg accataaaca aattttatgt atatcttgtg acttatactc ttcaatgtta
    29221 ttttcatatg ttattttttc ttggatatgt tgtttattta tacaagaatt agttcctttt
    29281 aactgattca ttgtttcaaa tatttctttg gctttttgat tcatgaaaac tttttcctga
    29341 ctctcaatcg aaaaagcaac ttctctcttt tttaataaac aaacaacttc ctttatcaat
    29401 ctttgattaa aagattgatt ataaagtaat tcaccatgat attgaatgta attacctcct
    29461 cctgcaatat atccatcaac tcctaatgat aaaacatcat cttgtattgt acccatactt
    29521 cttccagtac aaataacaac actgcaatga ttcttttgac ataaacgtat agcatgcttt
    29581 gcagattctg ggattccata tacctcatct ctaagcgtac catctatatc aagtattaat
    29641 aatttgtaca atttcatctt cctttcttac tatcaaaatc ataatagcat tcaaaaaaaa
    29701 agatatcaat attttgatat cttttaaaaa atatgttcat ttttagcaca aattttcatt
    29761 ttcggggaat atgttcactt tctaaataca tcttgtattt aatataaagc agttcaatta
    29821 aaataaaata aggtgttgtt acatcgtagt ccagttgttt atctacagaa atagatatag
    29881 attgaatata caggttttca tcacataatc ttgctaaagg attacttatc atttttgtaa
    29941 tagaaaccat ataaattcct ttcattctca tttctttagc aaaatcaata attgtagatg
    30001 catctccatt caatgaaata aagaaaatca tatcatctgt ttttataaaa tgattaagtg
    30061 cctgaatcat atcataacca taaacatcat atataatttt ccctgttggt aagaaaatcc
    30121 ttttcatctc tttagcaact ctttcctgag aataaccttc tccaaagacg attatacgat
    30181 ttgccttttt cattctttca aacataatcg aacaatcctt cttctctata tcatctatca
    30241 ccttattata acaatccatt aattggtgtt tactttgatt tttatattct acttctgttt
    30301 catttaagcg tataataact cttaattcac tatatccagg taattgtagt ttttgagcaa
    30361 aacgagaaag tgcacttgta gatatatggt atttgtctgc aaattcatct atagaaagtt
    30421 taatacaatc ttctttatga ttaagaatac actcacagat atacttatca tttgctgtaa
    30481 atgaattata atatttattg aaaagtcctt caattttcat tcttttctcc ttctatactc
    30541 tatgtaattc atatgaagca aatctaatag agcataaaat gctcctacga cttcataaga
    30601 tatagcttgt aatgtatcaa gtttttgcgt ttcaacatac agattatact cacaaagtgt
    30661 tgcaattgta ttattatcaa gcctagttaa agaaatcgta tgtatatgat tgttgataaa
    30721 ttttgcaatt tcaattcctg cttttgtttc tccagacaaa gaaatgataa taaataaatc
    30781 atttttttga aatgattctc tcataaattc aatttcacct atatcaaata agtcaataac
    30841 acatttacct actgaaagaa atattctctt tatttcttga gctaaacttt tctgttcatt
    30901 acctgtccca taaatataaa ttgtttctgc atcatatatt attttacaaa tatcttgaaa
    30961 tgatattttt tgtaattcat tcattaatag tcgataattc tcgcaaacat catgaaaatt
    31021 attatcatta ttaacaaatt ctagttgatt atttttaagt aaatacttta aatcagagaa
    31081 tgaatctaat ccaatttttc tacaaagcct taataaagta gctctagaaa cattacattc
    31141 atttgcaatt ttttcacacg aaaaattacg gtattctctt tgacaaatca atatttgttt
    31201 aataataatt ttatcatttt catttaaatt atcataattt tggttaacta acgattctaa
    31261 tctcattttt tccactccct aaatattatt ttaatataca ttagttgctt tttctaggga
    31321 agagttttaa ataataagga taaaataatc atttgatttt atttttatta aaataaaaat
    31381 aacctgctaa aagcaggcac caaaaagtac ttatcctttc gaacatgtac tttagatatt
    31441 gctttcttcg gaagcttcat ctcttcatac tctaaaatat agttttttct aactcaaaag
    31501 tattttccat ggtagaaatt actttaattc atctgccatt tactttttat cttatttaat
    31561 aattactatt acttgaatat ttacttcttt aagtaaattt catagttaac aaagctaaaa
    31621 ctattattat agatgttaaa atacaaaatt gtaagtattg ttataaatat gcttacagct
    31681 acaaatatac atgatatatt aaaaagaagc tgtaaattat tatcatacat acaagcaact
    31741 atgcctctat taatccatgt taatttttat tatttaatac aaggagttcc atgagacata
    31801 catatccatt catattttaa attatccaat tctcttatag atttcaccat ctttttataa
    31861 tcccaattcc atggattagg ataaggaact atatttccat tcttattttt gactaaatca
    31921 cctgcaaata atacagcttc aaaaatcatg cagacatgcc ctggagtatg accaggagta
    31981 tggataattt ttatattccc aatctgcata ttctctccat aaggttcaac atccttaatg
    32041 agccttctgg aaattgcctt tccaataaac ttcttaaacc caggtctatc cttctctcct
    32101 ataataaaag gtatatcctc tacatgagcc catacttttg ctcctgtttt ttctttcaat
    32161 aatttaatat tagatatatg gtcaacatca tgatgagtta ataaaatatg cttaatatct
    32221 gtaagtttaa tgccaagtgt ttttaattct ttaattatat ttttccccat aaaaggaaga
    32281 cctgtgtcta taagaataat ttctttatca aacacaataa aacaatttga gccacaagta
    32341 ctatctaaca ttaatacatt ttcttttatc ttcataaata aattcccctt atttggtatt
    32401 ttacctatat aa
//
LOCUS       DAMJYC010000039        32400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJYC010000039 DAMJYC010000000
VERSION     DAMJYC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.040"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..1056)
                     /locus_tag="PC472_001982"
     CDS             complement(<1..1056)
                     /locus_tag="PC472_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2727308.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSK"
     gene            complement(1034..2110)
                     /locus_tag="PC472_001983"
     CDS             complement(1034..2110)
                     /locus_tag="PC472_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2727309.1"
                     /translation="MKYFDYICKDDLEKIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(2174..3310)
                     /locus_tag="PC472_001984"
     CDS             complement(2174..3310)
                     /locus_tag="PC472_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2727310.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(3311..4873)
                     /locus_tag="PC472_001985"
     CDS             complement(3311..4873)
                     /locus_tag="PC472_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2727311.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(4960..7719)
                     /locus_tag="PC472_001986"
     CDS             complement(4960..7719)
                     /locus_tag="PC472_001986"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2727312.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(7709..8338)
                     /locus_tag="PC472_001987"
     CDS             complement(7709..8338)
                     /locus_tag="PC472_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2727313.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(8424..9002)
                     /locus_tag="PC472_001988"
     CDS             complement(8424..9002)
                     /locus_tag="PC472_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2727314.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(9041..9619)
                     /locus_tag="PC472_001989"
     CDS             complement(9041..9619)
                     /locus_tag="PC472_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2727315.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            9948..11384
                     /locus_tag="PC472_001990"
     CDS             9948..11384
                     /locus_tag="PC472_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2727316.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(11476..13350)
                     /gene="asnB"
                     /locus_tag="PC472_001991"
     CDS             complement(11476..13350)
                     /gene="asnB"
                     /locus_tag="PC472_001991"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2727317.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            13553..14227
                     /locus_tag="PC472_001992"
     CDS             13553..14227
                     /locus_tag="PC472_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2727318.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            14340..14876
                     /locus_tag="PC472_001993"
     CDS             14340..14876
                     /locus_tag="PC472_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2727319.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(15042..15662)
                     /locus_tag="PC472_001994"
     CDS             complement(15042..15662)
                     /locus_tag="PC472_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2727320.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(15976..18114)
                     /gene="vanT"
                     /locus_tag="PC472_001995"
     CDS             complement(15976..18114)
                     /gene="vanT"
                     /locus_tag="PC472_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2727321.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(18132..18938)
                     /locus_tag="PC472_001996"
     CDS             complement(18132..18938)
                     /locus_tag="PC472_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2727322.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(18935..20035)
                     /gene="vanG"
                     /locus_tag="PC472_001997"
     CDS             complement(18935..20035)
                     /gene="vanG"
                     /locus_tag="PC472_001997"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2727323.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(20296..21378)
                     /locus_tag="PC472_001998"
     CDS             complement(20296..21378)
                     /locus_tag="PC472_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727324.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(21428..22129)
                     /locus_tag="PC472_001999"
     CDS             complement(21428..22129)
                     /locus_tag="PC472_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2727325.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(22563..25094)
                     /locus_tag="PC472_002000"
     CDS             complement(22563..25094)
                     /locus_tag="PC472_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2727326.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(25202..25819)
                     /locus_tag="PC472_002001"
     CDS             complement(25202..25819)
                     /locus_tag="PC472_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2727327.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(26181..27065)
                     /locus_tag="PC472_002002"
     CDS             complement(26181..27065)
                     /locus_tag="PC472_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2727328.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(27256..28506)
                     /locus_tag="PC472_002003"
     CDS             complement(27256..28506)
                     /locus_tag="PC472_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727329.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(28728..29492)
                     /locus_tag="PC472_002004"
     CDS             complement(28728..29492)
                     /locus_tag="PC472_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727330.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(29486..30211)
                     /locus_tag="PC472_002005"
     CDS             complement(29486..30211)
                     /locus_tag="PC472_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727331.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(30213..30581)
                     /locus_tag="PC472_002006"
     CDS             complement(30213..30581)
                     /locus_tag="PC472_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727332.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(30757..32301)
                     /locus_tag="PC472_002007"
     CDS             complement(30757..32301)
                     /locus_tag="PC472_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2727333.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT        10821 a         5664 c         3368 g        12547 t
ORIGIN      
        1 tttactttta ataggataat tattcttatc aatattaata attggacttc ttgttgttga
       61 atgatagtat atatcatatt gattttcttt ttcttttgca aacagcatag gtatatacat
      121 aaattcttca gtacctaaaa atagaatctt ttctttttca ttttttaaag attctttact
      181 ggccatagtt atttttttac tttctctctt aacgatttct tttaatctaa attgttctct
      241 tctattaatt ccaaatcttc ctgtatattt tacatatttc ttatttccaa tataatcttc
      301 taaatcaaca taaatatgat ttactactaa atttacttta tcacttgaaa tagtctctaa
      361 tttagtaata ctataattat cgttgtaatt tgaaataata tcttcatcta tataaaattt
      421 aaagtttcct ttaaataagt atacaaaatc tatcttacaa tctagctctc tttctaaacg
      481 agtaacttta cttaaatttt catcacttac ccaattgagt attgagcata tagtgtattt
      541 ctttttagga taaatgcttt gaattttcct aattatattc atgcatgtat tggctgtggt
      601 aatctcatca tcaaccaata ttatctcatc tccaaattta aagttgttaa gcataccaaa
      661 gtataaattc tggtctgttg catgggaatg ttcctctaaa aattccagat tttctaactc
      721 gtcaaactgc tctctagtag tatgaataaa ttcataatct ccacataagt aatcaaaaaa
      781 actatgccca acagctgtac cagtttcagc aaaagctata acagtaccat ttgattcttc
      841 cttatttttt tcattataaa ttgtagccat taattttccc aaattatcca tctcacttgg
      901 cttacatgct aaatgtttac cgagtttctt tgagataaac aaaaaacgtc ttttgctatt
      961 atttcttatt cctacgtcta aaaaatcatt tagttctaaa ttcaatggat tatcaataat
     1021 gtcaatctca caattaaaat ttaagcaatt cttcatagtc tactccttta tttaatacac
     1081 catatatttt agatagaatc aatgtttttt tagcccataa aaaatgtgga tttgtttcat
     1141 tcattctctc tcctaataca cccttgctca caccatactt actgtctgta ctatccagta
     1201 tatccaaagc atcagtaaaa tcttcatatg atactgtaga tagcgcttgt acaatttgaa
     1261 tttgcattgg atgaattacg gtctttccaa ttaatccatg atatttatcc aataatattt
     1321 cttctatata cttatttctt atatccttat tcgtcatatc aaaaaattct gatactccac
     1381 cacttatagg tatatctaac tctggtcttt gaagatatgt gattatatct aatataaatg
     1441 aagaacaaat caaattatta tatatagaga agttcttatc tctccttata ccatacatcc
     1501 ctaatatatc agttatgcct actctaatgc ttaatattct tgacttatgt cttaaaatag
     1561 cattgtacaa attagtaaaa ctaagttctt ttttttcatt atatataaat tcttttgtct
     1621 ctattatcgg aataacatac atatcttgaa gattcataga atctaaagct tctatacaat
     1681 tttctattaa ctccgaattt gctttaggaa ttaatattcc tgttatagaa tgtctatttt
     1741 taattataat ctctttaatt ctcaacagtt gctccacatc ttttattctt acaaaaatta
     1801 gtggtattcc atctttatta gttatacttt cattgctaat attttttaaa attaatctaa
     1861 gattttctat agcctccaac tctccattta ctccaacagc atcttctaaa caaatagcta
     1921 atggtctaac tcccttaaca tcccctataa tactcttata aatcatatta tattgagtag
     1981 ctggaacgta taaaaaagcg cccagtgcat atttcaacac atctttttca gtcttagcac
     2041 taaaatcttc tggctccttc aaaaaaattt tctccaaatc atctttgcag atataatcga
     2101 aatatttcaa tgtctagcct cctcatatac atagaaaggg ccaactttat acaaattagc
     2161 ccttaatata atactactta ttataaatta tagtcgtatt taattcttcc aatctctttg
     2221 tattatcctt aattaaagct ctattgtttt cttctattct catagtttca tctataccag
     2281 atttgatagt attgtacatt ttttccaatg tttctatttg aacagaactt gttccagcca
     2341 ttctagctat ttgtgcactt tgagtactta cattttgtgc atttctaagc aataattcat
     2401 tagttttgtc atcaagtgct tttagagatt ttgcttgtaa ttcttgcttt ttaagtaata
     2461 tagcttgaga taaacattgt ttaaatactg gtaaagttac gacaaatgca ctatttattt
     2521 ttctaattaa accataatta ttatgttgca tacctctaag cataggtatt gtttggatag
     2581 ctatattttc tgctatcctt aaatcataga ctctttgatt taacatatca taaactttta
     2641 atagttcttg ataattgaca acatccattt ggtctccact agtctctgat ttttgtttaa
     2701 agaaaggtag tatttcatta tccatttctt ctattgccat ttctccagca acaatgtatt
     2761 tttgaagttc attgtaaaac tcaaagtttt catttaacat agcacctatt tgcttattag
     2821 aaagtgctat atctctttcg tattgttcaa gttcaatttg aattttctca acttctcctc
     2881 ccaatgtttc atacttttga aacatcattt caatggcatt attagctttt ttgaaaagtt
     2941 tttgaacgaa atttggttcc ttagtttctt gaaaatcgtc taaatcaaac ttgtccatta
     3001 ttttagttaa atgaaccagc attttagaat tttcttcatt tctatttaat tttattgtat
     3061 ttaaaaggct atcagaaact cttgctactc cttcagatgc tttcctacca aactgtaaaa
     3121 tcgtatctgg attttctact tctataaggg atgtcaatgc ctcaacttcc ttagactttc
     3181 tcagttcatt ttttttatta tttgtatatt ccattatatc gaagttttct tcttgaactg
     3241 ctgtaggcaa attctcagta acctctacag agttcttatc ttttgaaaaa ctatttatat
     3301 ttatccccat ctaaaatcgt ctcctctttc caaaaatttt tttaagtaaa ggtatatcat
     3361 tttcaagcga atttaattta aggtcatata ccattttatc caatctgtgt acatcaatca
     3421 attggttatt tataacattc tctatattgt tttctcctcc tggacataat atgataatat
     3481 caaatccaat cttactaagt aaaagcataa gaaaaactat ttctttatca aatacaatac
     3541 tcttgtacat gtatataatc aattttgggt ttataagtga gtaatcgaaa ttgttcatca
     3601 tatgaacaaa tttcttatcc atgtgaagta ctgtaaatag accttttatc ttgtcttctc
     3661 cacttaaacc attcaaaaac atacttcctt ctaacaactc ttccactttc tctaaaatca
     3721 taatttgttg attcaatgct agtgttgata tggaataatc tttattattt aaaacagagt
     3781 ttttatctat tgcacctttt gaatccaata aaaaggataa actaaaagct tctcttgtaa
     3841 aagcttttga aaatctatct acatctccat taaattcaat aaatgttgaa ctttccgaat
     3901 ctcttaataa atctaaaaac ttaatatatt cattcttatc gttatatatt ccattaatct
     3961 tagaaagaaa atttggagct tctacaatag catcattaaa cttaaatcct ggtcttaatt
     4021 taagtggttg attccagtat attgatattt catcaacagt tgaacttaga agtagatttt
     4081 ttatttttct atcttggagt tgccaaggtt ttataaatcc tgcatcattt aacaattcct
     4141 cacttatacg ctttgatgct tctgccccaa cagtaaaagc tttttttaca gaacttttat
     4201 ctattttttc ccctaaaata accctttctt caaaagaaat tttttcttct attatgttgg
     4261 cttcttcaaa ctctatattg tatctttctg attttaaaat atctatatta ctctttgaat
     4321 ttggattaag atataaaaca tcaaaacctg ccaagtataa tattaagagt aaataaactt
     4381 catgtttttt tatatctcca taaaatatta cttttggagc ttcattttta tttatatcta
     4441 aagcaccgat ataaattttt atccatgcca ttattttcac tataaaattt tgtttaactc
     4501 tatcattagt aaattctttt tcctcttctt ttaacactct atccattgct tgctttattt
     4561 tataatcaat ctcttcaaat cccaaaatat ttatatctac atcttccatg ctagttctat
     4621 atttttgaag tccactttta aaataattta taatatcaaa atcactagga tttgctataa
     4681 catcagtaaa taaagcaata tcttttttgc tatcaatatt ttgttttgtc ttaaatatct
     4741 cgagattgta caaaacttca tcttctaaac caatatacga aataaaataa cttagatatc
     4801 cctttgaagg agtgtaattt cctctatctg attggctgga gaatattttt tcaaatacct
     4861 ttgagtcacc cataactatc ctcccaattt aaatgtttat tcagaactga cccaaaattc
     4921 atattttgag tcagtctgtt ttcttctaaa aaatttgttt taagcagtct tttttggttt
     4981 taccagtctt attatacatt ttacaacttc attaaccaca attacacttg ctgctacaca
     5041 taaaactttt acccatgtca taaaatccat tgctacagag ttaaagaaat cttggaatag
     5101 ctgagtaaat attacttgtg caattcctgt caacactatt atttgtagtg ctaatttgtt
     5161 atgtgtaaaa ttagggaaga tactatctgc attaaactct ctacagttaa atgcattaaa
     5221 cagtgcacta aaagcaaata acgcaaacat tactgttccc ttatgcgctt catcaactcc
     5281 taagaaatta aatgtagatt gtaacattag tattgctgtt atataaaatg catttaatac
     5341 tattgtatac accattgact tagaaattat attggctttt ctatcaacag gttttctatt
     5401 taacacatgg tccctaactg gctctaaacc taatgctagt gctggtggtc catccataat
     5461 gatgtttacc cataataatt gtattgtagt aaatggcatc tcttgtccca taacttgaga
     5521 tattatagcc actaaaaatg caattacatt tacagttaat tggaattgaa cgaatctttg
     5581 gaagttatca taaattcctc taccccattt aatacctttt acaatggtac taaaactatc
     5641 atcagttaaa attatgtctg ctgcgttttt agatacctct gtaccagata tacccattgc
     5701 tataccaaca tctgcttttg aaagtgctgg agcatcattt ataccatcac ctgttactgc
     5761 aacaacatct ccgctctttt gcagagctga aactattctc attttactat ctggtttact
     5821 tcttgccacg attgaaatac tttgtatctc ttgttttaat tcttcatcat ctaaagtatc
     5881 tatatatgaa gcttcaacca ctctcatatt attttttaat aggcctaact cttcacctat
     5941 agctcttgcc gtatttatat tatctcctgt aagcattttt acagaaacac cagctttatt
     6001 tgctgtattt acagactcta taacatcttt tctaagtggg tctcttatac caacaaaacc
     6061 actaaatact aaattgtttt ccattgcact tacattcata gcatcacttt cagcagttac
     6121 tgctgcttct tcttcagctt gtgagatttc tttatatgca aaccctaaag ttctcattga
     6181 ttttatttgc aatttcttta tttcttctaa aatctcatgt tttatatttt cagttaatgg
     6241 aactatgtct tttcctcttt gaatatgtga acataattca agtaagactt ctggtgctcc
     6301 ttttgataat agtacatgat gattatcttg acctataaca gaagtcattc tctttgaatc
     6361 tgaagtaaaa ggattttgag ctactatatt agaatttttt ctaacttgtc tataatctac
     6421 gtttttgtaa tataaaagca atgcacattc agtagcactt cctaaatact taatatcacc
     6481 atcattgtgc tctacatcag ctgtagagtt tatcatacag ttttcttcaa aataactatt
     6541 tatttcctct tctgaacttt caatatatct gccatcaaca tatgcaactt ctacagtcat
     6601 tctattttca gttaatgtac cagttttatc tgaacatatt acagaaacag aacctatcgt
     6661 ttcacaagct tcttttttag taaccaatgc atttatttta gccatttttt gcattgttat
     6721 tgcaagtgtc atatttatca tagttggaag accttctggc actgctgcaa ctatcaacgc
     6781 aacacatact gtaaatgctg ttttaactgg gtctattgat tctaaaaacg gtaatattcc
     6841 agacatatct acatttaata cattcttact tatcatttgg aaaaccataa atataaatag
     6901 taatcctgct actgcactag aaactttggc tatcaaacca cctaaatttc caagttttat
     6961 ctgaagtgga gtttgctcat ctgtttctga taagttttga gcaattttac ccatttcact
     7021 attatctcca attgaagtta caactagagc tcctctacca taagctacaa gtgtgccacc
     7081 aaacaccata tttatctgtt ttgcaggtat tggctcctgt actactactg ctcctttaga
     7141 ttctatctct tccatagaaa ctactacatc acacttttta gatacgtcat cagattctcc
     7201 tgttaacata tcttctctaa ttttaaggtt tatactttct ataagtctac catcagcagg
     7261 taccatgtca ccagtttcaa tatatactat atcacctggt accaattcac tcttagaaat
     7321 ttgatgtact tccccatctc tcaatacttt tacttctatg ttttcagtca acttagaaag
     7381 agcctctgct gctttcttag aacgaccttc tgtaatcata cctattgcta tacctattgc
     7441 tatagcacct acaatcccta ttgcatcatg agtttcccca attactgcac ttactactgc
     7501 tgcaataatc aagataacta tcattggttc acttaaactt tcctttaaat catcccaaaa
     7561 actaccctct tcttttggag taaattcatt caaaccatat ttttctcttc tagtcttgac
     7621 ttctgattct gataaacctt tttcaggatt aacagataag tcttttgtaa cttcttcttt
     7681 atctacatta taatatttga tatcattatt aaattccata tgcacactca atcctgtcta
     7741 aattttgcat tacttcttta agacccgcat cttttgttcc ttcaccaatt gctttaaatt
     7801 gccaacttcc atcttttcta tatatagctc ctactataag agtagttttt tcagaataat
     7861 tatcagatag attgtatttt gctatctctt tcttattacc ttggtcttca actcttatat
     7921 aggcattttc tatcattcca aagtgttgtt ttctatctac acaattgtat atatttacta
     7981 caaacaataa tttatgaata tttgatggaa ctttatccaa attaactagg atttgttcat
     8041 catcacctgt accctcacca gttagattgt ctcctgtatg caaaactgat ttacaggcac
     8101 tttttagatt tccaaaataa attaaatctt ttattccact taatttacct tcttgattaa
     8161 gcataaatac agatgcatca caatctatat cagcttgtcc tccaccaaat aacgatttaa
     8221 aaaaacctcc gccagattgt tgcacagggt cccatcctag acctacaagt atgttactca
     8281 gtcctgggtt agattttttt aaatctattt tatcaccttt tgataaatta atcgacatac
     8341 taaacctcca caaaatttta tataatataa cctatacatt atttgtatag gttatttata
     8401 tttaatatat tatattgctt aaattaaaca tttaagccat agtttctaca aagtgcttct
     8461 aagccacctt caaatcctgc tccaactgca ctaaacttcc attctccatt atacttatat
     8521 atctcacata ctgtaattgc agtttctata ctaaattctt cacctaagtc atatcttaat
     8581 atttcttcat cattttcaga atttaatact cttatatatg agttacttac ttgtccaaaa
     8641 ttttgtcttc tttcagcagc ctcatatatt gtaactgtta cagctatctt ttgtatatgc
     8701 tttggcattg tctctaaatt cactaatatt tcctcatcat ctccatcacc ttcaccagtt
     8761 ctattatcac ctaagtgttc aactgctcca gatggatgtt ttaagttatt gtagaatata
     8821 aaatcttcat ctttttcaac tctttgagat tctccaacca tgaaaacact tacatctaaa
     8881 tcataatcat agccactatc aaatgaattt gtatcccatc ctagtccaag ttttatattt
     8941 ttaagacctg gatttccctt agttaaatcg attttttgtc ctttttttaa tactatagcc
     9001 atggaaaata tcccccttta gtttattgtt atagttgaaa ttataagttt attccaaagt
     9061 ttccacataa tgcagcaaga ccaccttcaa agccagaacc tagtgcatta aacttccatt
     9121 ctccattgtg cttatatatt tcagctacta ctatagctgt ttctatacta aaatcttctc
     9181 ctaattcata ctttattaac tcttcatttg tgtctttatc tactattctt atataagaat
     9241 tacttacttg tccaaaattt tgtcttcttt ctgttgcatc atgaattgta acagtaaaag
     9301 aaatcttgct tatttctcca ggaatcttag ataaatcaac aactatttgt tcatcatctc
     9361 catcaccttc gccagttctg ttatctccta gatgctcaac tgctccagat gtatgtttta
     9421 aattgttgta aaatacaaaa tctccatcat tagtaacact tccacttgct cctgttaaaa
     9481 atgctgctgt atctaaatca aagtcaaatc caccatcata cttgttagta tcccatccta
     9541 aacctactaa aatattttta aggcctggat ttcctttagt taaatcaacc ttttggcctt
     9601 ttgataatgt tatacccata atttatcgct cccttgttaa ttttagctaa acaaatcttg
     9661 ctaattattt atctttttct ttaatcatat tataacattt tttattcatt tgtgaagaaa
     9721 acttgcttga tatttttaaa atataattaa aaaaatgttt atatttctta ctttttacat
     9781 agattttaca tactttatta aatgcataaa agctatgttt aatatgtaat tatatatcat
     9841 ttatataaac aagtacatat tttattttga aactacaaaa ttattgtact atattgtttt
     9901 aagttgtttt ataatagatt atatcatctt attataggag aaaaaatatg atttgtacta
     9961 ttaaaccatt aaagatttca ataaaaaagt ctactgaacc tctaattgaa tttatatctg
    10021 atactaccta taaaaataag agttcttcat atattaaaga agtctacttt acaagatata
    10081 taggtgttga tgattcaatt gataaataca tagaaaaaat taaaaatata gatacttatt
    10141 tttttaataa tcctacaatt ccctatatta gattaaacaa tctaaaaata aattttgaca
    10201 aagaacaaac agataagatg cttaaaattt ttaatattta tgatagttct aagtttaaca
    10261 gttgggattt atataatatc gactttccat gtaaaataga aaatgacact ttaaattgga
    10321 ctaaaaagat tgcatttaaa aatactttag atttattttc cacaagtatg ccaaattgta
    10381 catcctctat catcaaaaac tttgcagtta aacttttgtg ttggatagac tattttctac
    10441 ctaaattatt ttataacaag gtgaagacta ctatttcacc aaagatagta tactttggaa
    10501 atattaaaag gcaagaactt tttttcttat attttttatc acaacttggg tgtgatattt
    10561 tatatataaa tccaaataaa gatattcttg atttatatcc tgaatgttca aaattttcta
    10621 ctctatttga actttctagg aagactccaa atatattaga aatacctttt gaaaggcata
    10681 ttacaaatat aaattacagt gaaaatacca ataagacttc tcctactacg aataaaccta
    10741 actctaaaaa tacacttaat aacacaaatc taacatctaa aaaaattgta caagatgagg
    10801 aagtagaact ttcttatgaa gaccttgcaa agttatcagc ttctgttgtt atgattgttg
    10861 tgtgcaataa cgaaaataaa ccgtttaaga gcggttctgg agtagtaata aataatgaag
    10921 gttatatact tacgaatctt catgtagtca atgatgggta ttcattttta gttagatttg
    10981 aaaatgatga taaagtttat acatcttacc aaattataaa ataccattca gattatgatt
    11041 tagctgtgat aaaggttaat agaaaatgca agccaatacc tgttaaagtc agtaaaaaac
    11101 ctgtgagagg tcaaaaaata gttgcaattg gaagcccttt aggtcttttt aatacagttt
    11161 cagatggaat tatctcagct tttcgtgatt ttgaaacggt tcaaatgata caatttacag
    11221 ctccaatctc tagtggtagt tcaggaggag ctcttttgga tatgtttgga aacttgctag
    11281 gattgatttc cgctggatat gatgatggac aaaacttaaa tttagctgtt gaaagtagtc
    11341 ttgtcaaaat atttgccaat aattttattg agatagtaaa ctaatcaatc aaaattctac
    11401 ttatcctatt ccataataat caggagggtg aaaacattgc atgcttttgc cctctgatta
    11461 atattgaaaa cagtattaat actcgacatt tttatgctca ataaataatt gatgccatag
    11521 acaaaatgta aatatagtcc atatttttct cgaattatct ctatgacctt taagatgcgt
    11581 atctaaaagt tttattgcat attttttatc tataaattca tctacatttg aattataaat
    11641 agtttccttt acaatatccc ctagagaatc ttttaaccaa actcttattg gagttggaaa
    11701 tcctaatttt ttcctttgaa caacatgttc tgggactatt tctctaaacg cctctctaag
    11761 taaaactttt gtattattct tatttatttt ttgctccatt cttaaatttt ttgctacatc
    11821 aaatacatct ttatataaaa atggtactct aagttcaacc tgctcagctg tagaaagttt
    11881 gctaattttt tgaagtatat cgccttgaag ccatgtattc acatctatat gttgcatttt
    11941 acttatataa tcgtaattat ttttttgtgc atctctatac aaattagaca acaggtattc
    12001 atatatattc ttttccttat acttaaaaaa gaatcttttt acttcgctgt tttcaaaaac
    12061 ttttgcattt cctatatatc ttttttctaa tggtgttgta gctctataaa gataatttct
    12121 tccttttatg tttggcataa gttctgaaac tcttcctaat atacttttaa tataagtagg
    12181 tgaattgaca actgatttca tagtactata ctctttatat atattatatc ctccaaataa
    12241 ttcatctgct ccttctccag acaatgctac tttaacatgt ttacctgcct ctttaattaa
    12301 gaaataaaga cctacttctg aagggtctgc tatagggtca tccaaagaat aaattacatt
    12361 cggtaatgat tttatatatt catcttgtgt tatattaatc tggatatttt ctatacctaa
    12421 cttatcagct gttttctttg ctacttctaa ttcactatat cctttgacac taaagcctgc
    12481 agaaaaagat tttatatttg gattaatttg tgaagccaca gtagttataa tagatgaatc
    12541 tattccacca gacaaaaatg ttccaatttc tttctcctct tccatatgag ttactattga
    12601 atctactact atatttctta tatcatcctc gttcacatct ctattgccta agtgattttt
    12661 agtataaaaa aattttgtac tacttctaaa ctcaagttta ttatatctct ttaaagatag
    12721 tataccattt tctaccctaa aatagtgacc tgctggtatc agccttattc cttgtatcat
    12781 agtatcttca ggaagtacat attgaaatga aaaataactt tgtaagcttc tttcatttat
    12841 atttaaattt ttcatatatt caagtaaaaa cttatactct gaggaaaaaa ttattgcatt
    12901 ttccttattt atataataaa gtggttttat cccaaataaa tctcttgctc caaatatact
    12961 ttcattttct ctatcataga ttataaagtt gaacattcct ttaatcttat gtacacaatt
    13021 cttaccaaat tctatataag atgccaataa gacttcaata tcatcatttg tagtaaaatt
    13081 gtgtcctttt ttaattaatt cttcttttat ttcatgatag ttatatatat ttccattaaa
    13141 aacaataact tttccatctt tttcaaatgg tttatttcca taaatagcat caattacatt
    13201 aaatctttca aaatcaaaag ttttttcctc atccttatac tctattctac tagaaagtct
    13261 tgtattattt acagattctt ttattttaaa tttaatatct tcttttttag atatatcacc
    13321 tttataataa attgaaatat tgccgtccat ataatcaaaa ttcctttctg tcctttagtc
    13381 ttattctata tttcatagtt ttgtctttgg ctttaaaact tattcaattt aaaatgaaat
    13441 tgctattaaa ttcttataaa ttatctgtta atatttcata ttttttttat ttctagaaat
    13501 actatattag gaggtgattt ttatgaagaa aaaaatatta ataccagtta ttatgtcttt
    13561 atttataatc tcacagtgca taacttcatt tgcttttaca cctgaaaata acaaatttaa
    13621 ggttaaacca ttaccttatg catatgatgc acttgaacct tatatagata aagaaacaat
    13681 gaaactgcat catgataagc attatcaagc ttatgttgat aaattaaatg ctgctcttga
    13741 aaaatatcct gagctttata attattcttt atgtgaatta ttgcaaaatt tagattcttt
    13801 acctaaagat attgctacaa ctgtaagaaa taatgcaggt ggagcttata atcataaatt
    13861 cttttttgat ataatgacgc cagaaaaaac cataccttct gaatctttaa aagaagctat
    13921 tgatagagac tttggttctt ttgaaaaatt taagcaagag ttccaaaaat ctgctttaga
    13981 tgtctttggt tctggttggg cttggcttgt agctactaaa gatggcaaat tatctattat
    14041 gactactcca aatcaggata gccctgtaag taaaaaccta actcctataa taggacttga
    14101 tgtttgggag catgcttact atttaaaata tcaaaataga agaaatgaat acattgacaa
    14161 ctggtttaat gtagtaaatt ggaatggtgc tttagaaaat tacaaaaatt taaaatctca
    14221 agattaatta aatattaaaa tccttttgaa aaaaacttat gtttgctata taatataata
    14281 aaagatagtc taaatttacc aatataatgt ttatagtgac gattcaggag gatttttata
    14341 tgataaaaga aacacatgaa aggggaggtt atatattcgc acttttgata ctccccttta
    14401 taaacaatct atacctagtt aaatacgata ttgcatataa aatagtatta attataattt
    14461 atatttattt tgcatattta ggttctttat ttccagatat agatatgaga ggttcttaca
    14521 taagtaaaaa atttatgtta atatacaaat tatttggaag taggtttaga catagagggt
    14581 ttactcacag tctaattgcc ttattactta tttatagttt ttttaaatct ctgacaatct
    14641 tcactaacaa taatatagtt ttctcatgtt tgtccagtgg gtttataata ggatatctat
    14701 ctcacttgtg tcttgacctt attactaaag aaggtattga attgttttat cccattacca
    14761 taaacatttc tttactacct ataaaaacta actcaaaaac agaaaaattc atatctaagc
    14821 tattaaattt catagttatt tttttgattg gatatcaatt ttatatttta ttatagttgt
    14881 agaaaaaata aataacaata ttttgaacta agtataaata agttttatat attttagttt
    14941 acaaaagaac taaaaatttt gtaccttaat acaaaaaata aaacgtatct taaaataatt
    15001 tagtactaaa tcattttaag atacgtttta tttcgtacaa gctaaatcat tttatcatct
    15061 ttatcattgt gatgtttttt atgatggaaa tcataattat gattttcaag atttttattt
    15121 actctatttc caatcttcca tgacaaaaac accaataata taaatgatgc tatctttaaa
    15181 ggacttgtta taaaaccttc tatatcactt gccacgtaac ttataactat aaacattaca
    15241 aattttcctg ccaacattgc tggcacaaaa tcttttaaat cttttttacc aaaagctgat
    15301 gcgatagtaa ctaaacaacc gggcataaat ggacaggcat acgctataaa taataactta
    15361 aatccctgtt tatccatcca acttattgct ttttcagttt tactatttct caacttatta
    15421 aaaagcttac tatcattaaa tctgcttatt aataagaata atgaagttgt cccaagtcct
    15481 gagccaatcc atgataacaa acatccaata aataatcctt gtattgcagc atttgctgta
    15541 actatagcaa caagtggtaa ggctggtata aagctttcta taaatgcact taataagccg
    15601 attattgtgg ctaacaccca ataattttga gtcaaatcaa gtagttcttg tatatactgc
    15661 atattgcttt tccccttttt taactttaac tattattata tactctatat gtttattata
    15721 taccaatctc aaaaaatgaa tctaatatta aaaagtaaat aatctatata agtttacagt
    15781 taaggtataa ttattttatt atatattata acaaaaaagg tgatgtttca aaataaaaat
    15841 ctgaaacatc acctttttat aacttaaatt gtcatgtaag ttataaaaaa tatatattag
    15901 acataatatt actaaaaatg tctagtcaga gtaaaataaa ttacacagta caacaaatat
    15961 tactatttaa ttgagttact tctttataac atttaacctt cttcctactc tgctcaataa
    16021 ttcattcgat atactacatg agttatttgc aacttcagaa gcagataatt cagataaatt
    16081 atctcttcct attattattg ccgtattacc aacttctaca ttaggtatat ctgtaatatc
    16141 aacagcaagt tggtccatgc aaattcgacc cactattgct gctctacaac catttattat
    16201 tacgtaactt tttccacagg ataaatttct aggtatacca tcggcatagc caatagataa
    16261 tatagcaatt acgctatctc tgcttgcaat aaagactctt ccatatccaa aactatcatc
    16321 tttttgtatt tttctaataa gaattacttg tgattttaag gataaaacag gtcgtaaatc
    16381 aagttgcaat ttagtcgtat catttggaga acttaatatc ccgtataatg caattccaat
    16441 tcttacataa ttacacttaa gattgggata attaaaaaaa ccataactac tttgaatatg
    16501 tactttagga atttttatac ctttttttaa cagaaaatca attaacttat aaaaacattc
    16561 aatttgttta tttgtaaagt ctacatcttc atctttcaga ctatctgaaa cacacaagtg
    16621 tgtaaatatg ccacatatat ctaagtattt taatttaaat gcctttaaaa ttgacctcac
    16681 atcattaaca tcaaaaccca gtctgtgcat acctgtatca attttaatgt gtgattttat
    16741 gttgtatttc tgtttgttta aagagatagc atgattaagg tcaataacac tctgtgtaag
    16801 attgtattta tgaagttcct ttgcccttat tgggttagta tatccaagaa tcaatatttc
    16861 tcctagtatt ccatatcgac gtaaatcaat cccctcatct attgttgcta ctgcaaatgc
    16921 ttttacacct atttggtttg cacaagtcgc tacttcaaaa gcactatgac cataggcatt
    16981 agcttttaca actgccatca attcacaatc ttctggcata gctttttgta tcatctttac
    17041 attatgttcc aaattattta aatcaatttc aatccaagct ctattggtat ttaccttatg
    17101 ttctcctttc aagtcttttt ttagaggttt aagaatcaat ataataatta tagaagctat
    17161 aaaagatgct atacttacca ctatataatg aataaggcta ttgtcaacca agacagtttg
    17221 tattccgaat attttagaaa acatacgaat aacaacaatc atcataggat gtataatata
    17281 aataattaat gatgatgttc gcaataacct agctcgagaa cctttcaaaa atgttaaggc
    17341 tgtaaataag aaatacatac atggcaataa cataatatac atgctatcat gacgctgtac
    17401 ccctagttta tgtaaaaaca ttccctcaca taacataagt aagaatgata taagaaatcc
    17461 aatcaagcta tttttagttg acatataatt agatttatta gatattattc caccaagtat
    17521 gaaaaataca ggtgcaaaaa atataccatt tcgtgtataa tctgatactt caaaaatata
    17581 cccatataaa ttctttaata gtggtatttt ttctaaaaaa ccataataac tatcaccaaa
    17641 cataccaatt acataaataa caatagtgat tactagtgag tactttaaac ctagtctttt
    17701 tacaagaaag tatgcaatac ttgcaccaat aattgatgca ggtagatacc ataaatgata
    17761 taaagtccca tcaaatacaa tgtccttaat aatattgggc actaaattat tcatggaaaa
    17821 atatccagtg taaatattta agggaatata aattacaata gctatcccgt aaataaataa
    17881 tacatttttt agaaaagatt tcagtttatt acaattataa ttatatgttg aaattagaaa
    17941 aaagcctgat agcataaaaa agaatggcac agcaatacga gcaattatac tagttaacac
    18001 aaagtcagcc attttactat atgataataa tggagatgta tgaattgcaa ttataagtaa
    18061 tgcagctata atcctaaaat aatcaagccc tgcataagat ttattcctaa tcattcttgt
    18121 tcctccattt cttagtcctt tctatttctc caaaatgtca caataaaacc atccttattg
    18181 ttacccgata tttgatatac acattgttca ggtatagaga ttctcacttc ttctttagta
    18241 tgaataggca cataaaagat ttctataatt ttttctttat cttctatttt taaacaattt
    18301 gtatacgaaa atctcttaat atattctata tattcttcaa gagcgagatt cattttattg
    18361 ataatctcag aatgcggata tcccacataa cgaaaatgcc atggttcttg agatattcct
    18421 gttatttctt cttttttaca ctgataacgc tctataaaac cataatgtag tgctgcctta
    18481 cgaaactcat tacaaatacc attataagga aattcagggc agataaaatc tataacttct
    18541 ttctttaacc ctaaatcaat agcaagccct gtctgatgtt cactgtgatt tggaagtgct
    18601 acaaactttt tagtaaagtc ttttccattg tcacgcagag aacttgaata tatatcttgt
    18661 tgttcctctg cagtacgata tccacttaca ggtactattt catttattcc acatatactt
    18721 tttaaaatat tttgtaaaat gtttgtagct tcacgtttca taagaatact tggaaacctt
    18781 gtatctacag gaattaaatc tatatcttca gatgttttaa ctggtaaaaa agcatttact
    18841 agaattagat tcccatcata aactttatct ttagtcaata agatagtatt tgtcatttta
    18901 tcttctttat ttttatctaa taaaatatta ttcatcattt tatgtacacc tcaattatct
    18961 tgtccaatat ttcttcaaaa gacaatccaa tacctttcat catatttgga taacgactat
    19021 gagaagtaaa gccaggaatt gtattgactt cattaaaaat aatttcacca gatggagtta
    19081 aaaacatatc tactctagca aatccagaac atccaagagt tctataaata atttttgctg
    19141 tctcttgtat tcttttctca caatccaaat caattctagc tggcatatga atttttgaac
    19201 ttttcaatgt atatttttct gtataatcaa aaaatccttt tgacaattct atttcatcta
    19261 ctcttccaac aagtaagtca tcgtttccta aaactgcaca ccctacctca aaaccatcaa
    19321 tattttcctc aattattact tcattatcat gttcaaaagc caagtctact gctacttgta
    19381 actcttcttt tttataaatt ttactaatac caaatgatga acctgcacgc acaggtttta
    19441 taaataatgg ataagttaag tcttttgaca gttcagccac attaaattta tctgagtatt
    19501 ttagtaatat tgattttggt actttaatac cttctgaatg aacaagttta tgtgatttgt
    19561 ctttgtccat acataatgct gaagaaagag taccacatcc tataacctgt atacctgcca
    19621 attcaaataa cccctgcact gttccatctt ctccattttt tccatgcagt acaggaaaca
    19681 caaaatcaag tttagtgtat ttaatatcat tttcacatga ttcaatagca ctatttggaa
    19741 tatgattctg agaaacagca tcaggaatta aaaattcaat aataccattt ttgacacggt
    19801 tctgcgaaac aataactgga attatatcag tatccctttc aaaccaagtg ttattctgga
    19861 tattattata atctccaata taatgatacc aattaccctc ccttgtaatt ccaattggta
    19921 taatatcata tttttccgta ttcaaattct caagtactgc aaaagctgat tgtaatgata
    19981 catcatattc tgttgagttt ccaccaaata taactgcaat ttttttatta ttcatctttg
    20041 attctccttt gttgatttat ttatttttat acaatctaca gaaacatgtt aaaatacttt
    20101 ttctatttca aatccaatca accttcactt acaattgcat ctgttccaca taatatattt
    20161 tcacattttt ctattgatat aattactgta tcaaaataaa agcactcttg ttttgataca
    20221 gtaattatat caataacaag agtgctcttt atttatttat cctaaagaat ttcctatttt
    20281 tttatactta aattcttaca aatttcctac aatcggaata ataaccttaa agataattat
    20341 attgttctcg ctatgagctg ttattgtccc gttatgaagc tccactattt ctttagctat
    20401 agcaagtccc aacccagctc taccactgtt tgaattacga gatgtatcca acctgtaaaa
    20461 ttgttcaaaa atacgctcaa gtttttcctc tggaatcgta ttgctatgat ttataaattt
    20521 aagctttata taatcatcct cctgtacagc tgttatttca atagtagtat cttcaaaact
    20581 ataataaaca gcatttcgta ataagttatc aaatacacgt tgcattttat taacatcaca
    20641 ccttatcatc atatttggtg taacattcag tgtatatttt aaattcttac tcatcaaagt
    20701 aggtttaaac tcatatgtta gttgttcaag catacgagtt aagcttactg tactgtattc
    20761 aagtgatata tttgataaat taaaccttgt aatttcaaag aattcattaa ttaaatcttc
    20821 aagacgctct gccttatcca acgaaataga taaatatttt tttcgcagtt cttcagaaat
    20881 ctgactttca tctcgaagta gtgtcagata ccctataact gatgtaagtg gtgtttttaa
    20941 atcatgtgca agataaacaa taagttcatt cttacgttgt tctgccattt gtgtctcaaa
    21001 tttacgttgt tcaagtatat gtttaattga gtttattttc ttttctattg ccaataactc
    21061 tggagataaa gccacttctc caacatcttc tttgataaga gagtcaatac cttgatttat
    21121 ttcactaaaa tattttgtaa accaatttaa ataaacccgg aatattataa aaaacacaat
    21181 caccatagct agtatcatga agagctccat gctattacgg aaaatctgct gatataaatt
    21241 caatgcagct ttatattcca tacgaaaagc attttcaaag aatgatacta tccagataga
    21301 aaacttacca tacaatataa aagaatataa aatccatatg aaaataatgg ctataaatgc
    21361 cattaaagct gttctcataa aaattttatt tctcacttga gtaaagtcag gattgacttt
    21421 aatattttta cttttcaatt gtataaccta ctccccatac agtttttata aatttcgggt
    21481 ttttagaagg ttcatgaatt ttttcacgta atcgccctat atgagccata actgtgttat
    21541 tattatcaag atatttttct ccccatacag cttcaaacaa ttcttcagaa ggaaccactt
    21601 ttccttgatg ttcacataaa taccaaagaa tagaaaattc aatcggtgtt aatgctactt
    21661 ctttcccaaa taaactacat ttatgtgttt ctttattgat aatcaatcct ttaatatcat
    21721 actcattaac aattatcgac tgttgttcat aagaattatt atatcgcata tatcttctta
    21781 attgtgtctt tactcttgca acaacttcca aagggttaaa tggctttgtt atataatcat
    21841 cagcacctat ggttaaaccc attatcttgt ctatatcttc aattttagct gttaacataa
    21901 tgataggata atagtatttt tctcgaattt tctggcaaat atgaaatcca tcaatatcag
    21961 gaagcataat atctaaaata gccatatcca aatttttaga ttcaatacat tctagagctt
    22021 ccttacctgt ataaaacttg tatactgtaa aaccttcatt ttttaaatat acttcaatta
    22081 aatctgctat ttctttttca tcatccacaa tcaaaatatt ttcgttcata gattattttt
    22141 cactctcctt attatcgagg tcatcattta taatattggt aaatttacat atcaaataat
    22201 tacaagcata tttgtaacta atttttataa gaaaagaact actacatcag aatgtgttga
    22261 tgctactatt ggaacacaaa aactgagatt aattgttatt ataaaaactc ccctaacacg
    22321 tccttattat cgcataacag gtattaactt tcaataaaaa tatctatcag tcaaacacaa
    22381 ttcaatttat tataccagag acaatccata taaaaaacaa ttaaacctat gtatgtaacc
    22441 acagacatta ctaaaatttc catgttattt tatataaaaa ttcaaaaaag gagttgaatt
    22501 taaaatttga ttagaaaaaa atcaattttg agacaactcc tttttatttt aatgtattta
    22561 aattatagta tatcagcctt cataatatcc tcaaaatcac ctttttcaga tattaatctt
    22621 atcaatttaa cagtacagct taaatcattt ataagtatag ctccaactaa tgaaccatct
    22681 ttcataaata attttttata tattctttga ccttcatctt ttgatgaaat ttctttatca
    22741 aaattttgta tattaccaat actaaatact tttacattca ttgcatctaa tgaaactgga
    22801 tatacttcac tttcaaatgt caaattatct ccacttgcat ttgcccctgc tatttttccc
    22861 atttctactg aagatggcca tattgctaaa ctaatatttc ccacttgtgc aacatctcca
    22921 caagcatata tatctttaac atttgtctgt aatgtcttat ctacaacaat acctctatca
    22981 atttttacag atgtatcctg aaccatttga gtgtttgctc ttacaccaat tgaaaatact
    23041 accatatcac agtcgacact atctccatct ttaaatacaa ctttttgagc ttttccatca
    23101 ccttcaatac catcaactgc tacacccaat cttacatcta ttcctgtatc tcttacacat
    23161 tgttctaata ttttactacc ttctttatca agttgttttg atagtatgct atccatggct
    23221 tcaactacta ctacttctaa tccttttagt ctaaattccc atgcagcttc aagtcccaat
    23281 aaaccgccac caatcactac aactttctta gatttttcta gtgcttcttt tacactatat
    23341 aaatctttta tatttctaag agtaaacaca ttttctaaat catggccctt aattggtggt
    23401 ataaagtttc tacttcctgt agctattaca agcttatcaa atttaatagc tccatcattc
    23461 actataatag ttttattcac ttcatctaat ttatctacat tcgttcctaa tattactact
    23521 atattatttt tatcatacca atctttatct tccatgtaga aatctgaacc aagttcttca
    23581 tttataccat cagaaagtgc aggtctatag taagtcaact catcttcatt agaaatcata
    23641 gttatcttac aagttttatt tctttttctt atagcatctg ctgcataaaa accagctgct
    23701 ccatttccta ctattacgaa atattcatca gtatcttttc taaaagtaac ttcatcttct
    23761 tcaacttcta taaattggtc ttccttagct ccacatacag ggcatacact aggagcacta
    23821 tcaccttcaa aaacttcatt acaaattaca catttaaatt tcttagtttt ctttttactt
    23881 ccaaaagtag aatttaactt atctaggaat ttacctgtat aagaatataa tttatcaatc
    23941 tcttcatttg atggtttaaa gttaacagca aaaggtttta ctgctgtcat tctaagctga
    24001 cttattctct ccatagcatt ttctatacct tctccactcc atccatatga accaaatact
    24061 gaagcaactt ttccaccatg aagaactggg tttaacgata ttaatatatc ccatataggc
    24121 tttaacgcat ctcctaatat tgttggtgtt ccaaataata caccttgtga tactgctatt
    24181 tttgccaaaa tatcttcttg cttatgctct attacatcaa ataaatttac tttatagttg
    24241 ctattttttt caatatatgc ttttatagct tctgccataa tctttgtata tccatgtgca
    24301 gaaacataac aaattgtaac ttctttttct aactttattt gttcatttac actccagttg
    24361 taataaaggt ctataatttt tctaggattt tcagttaata ctggaccatg acctggacat
    24421 acagtatcta tatctaaatc ttttatcttt tctatagcag ttaccataga tggtttaaat
    24481 ggacccatta tacaatcata atagtatctt aaagcatcca agtaatcctt ttcttcattt
    24541 tcatcaagtg tatttactat cttatcatta ctatagtgac taccaaatga atcacatgtt
    24601 acaagagttt tatcctcttt tatatatgtg tatatggtat ctggccaatg aagcataggt
    24661 acagagaaaa attctaaagt tttgtcccca attgataatg tatctccatc tgtaactgca
    24721 acatattcaa agtctttgtt tacgatttcc ttaagatatt taattgctgt ttctgaagct
    24781 actacttttg catttttagc taaatccaaa agcttttcaa cgcttcctgc atggtctggt
    24841 tcagtatgac ttacaacaat atagtctatt ttttcaaaat ctatatttaa atcatttaat
    24901 ctttcaatat aattgtcaaa atgcttatct ttaactgtct caaataaaac tgttttttct
    24961 gttcctttta atacatatga attataagta gttccataag gtgtatacat aattatgtca
    25021 aatactctta aatcagggtc aagcgaacct acccaatata aattttcttt tatttttagt
    25081 gatttcaatg acataccgta ctcctcctaa aatagttatt tagtctcttt ataccctgct
    25141 tgttatattt taaacaaaat ttattcaaaa aagaaattat tttaaagata gtttaaatct
    25201 tctaatcttc tttttctacc ttcaatacag ttcccttttt agaatctact tttatgtcat
    25261 attcattttt atctttatct attcttattt tataaaaagt aacatctaaa tctttgtcta
    25321 aactccattc tttaactatt ccattttgtt cttttaatgc aatttccata gcttgttgag
    25381 gtgtcattat gtcatttaaa tctaattttt cttttctagc aactccattt aaatcttctg
    25441 aatccaactt ttctgtttta tcttctaata catcttttgt attagcatca attttcatct
    25501 tatattcatt attatcatct acaccttcca cagtataaac aaatttattt aaatctctct
    25561 caagacttaa atctactata tcagcatcct tatatttatc tttaaatatg tttattgcat
    25621 ctgtgtatgg cactttaaaa tcttgttcca ttaaattttc attatttttt tcatctttta
    25681 aagcgttatt tgatgcattt gcctcctttt ttgtattatc ttgtgcttgt cctgaatcct
    25741 tagttggact cccactacat gcagttaata aacccgctat aagcatactt accactaata
    25801 aatacgtttt tttacacata aaaaccctcc ttaaatatat taatttattc tatattatgc
    25861 cctctaaacc ctttatttac ccttttatat taaaaattcc tcctatttta tcaattttaa
    25921 aactttatat ttactaaaat taaagttaca taaaaattat actgtctcaa aatggaaaca
    25981 agcctctttt tcattgttga aataataaaa atatatttta tttcgtacaa aaagagtgcc
    26041 tcatgaacta aaaagttcat tttgagacac tcccattttt ttatattttt acttacaaaa
    26101 taacagtact tattaatttt ttacaaacct tataagtata tctatatgat attcttaaat
    26161 ttaaacacta tattaaaaaa ttaaattttg tttaattctt ttaccgttaa atccatttta
    26221 ggatatggaa tcgtaattcc ttcttcgtca aacctaactt taacctcttc tagtaaatca
    26281 agataaattg tccaataatc ttctgtcttg caccaaactc tagttgcaaa tttaatagca
    26341 ttatctccat gttctactac tcctataaat ggttctggtt catttattat taatttatga
    26401 ttattaacaa tatctttaag tactcttcta acatgtagta tatcatcttc ataccctaca
    26461 ctaaaagtca aatcaactcg tctagtattc tttgctgaat agtttattag actatcattt
    26521 gatacagaac cattaggtat aagtatctgt ttattatctg gagtaaccaa ttgtgtataa
    26581 aaaagaccta tttgttcaac cgttcctcca tgacctgctg cttctacgta atctcctact
    26641 ttaaaaggtc taatcaatag tataataaat cctccagcaa aatttgatag actaccttgt
    26701 aaagccaaac ctattgctac ccctgctgat gctatcacag cagcaaaact ggacaaactc
    26761 attccccaat attccagaac aatgattata acggcaactt taagtacact caaaagaaga
    26821 gactttaaaa actttcttaa agttaaatca atatctcttt tactaagtaa taacataaca
    26881 tggtttacaa atttttttat tattttaaat ccaatagaaa gtataagtat gcctattata
    26941 agttttactc cgcttgtagt agcccattct agcagacttt ccattatacc tcccatatcc
    27001 atcttattaa tatctaaatt atttaaatct aacttgctta tatcatctat tttcgtcgta
    27061 tccataaaaa actccttacc ttatatttta gaaccaatat tatagtttaa agttcttatt
    27121 ttctatttaa tataatacaa taccatttgt acatagtaaa caagatttat attccaatta
    27181 tacatattag gaaaatgcaa actatttgtt atatatatgc aaaatataat actaaattaa
    27241 actattaata aaacactata tagccttaaa atcatcattt tttaccccta tatgagatga
    27301 tactatagtt ataatactta gtactataaa tactacaaaa ccttttttat atccatcaac
    27361 tatattatat gaatttactt gtaaagagat agctataatc aaattaataa ttccacttcc
    27421 tataaattca aaagtattaa taacactagt tgccagtcca gaataaattt ctttattttt
    27481 atacttggca tcattaaaaa cctgtagatg tgacataact atacatccca taataaagaa
    27541 tgatattggt aaaactataa ttggtggttt tacctggtat acaactacaa ttacaatcca
    27601 tataaacaaa tctatcattg ctccaaattt tattatatta aatttgctag accttatttt
    27661 agcaaataca aaatccataa ttattgaacc aaatataaat ccatatgtaa aaaatgatac
    27721 tataaacgca gaaaaactct tactcacacc ataaacgtcc attatgtatc ttactcccca
    27781 caagcttata aatgcacttg taagaccaac aaaagagaac attatcattg aattatacca
    27841 tgttgatttg ttctttataa cagacttaat accatcaaca atattaacct tctctgactt
    27901 ttcatagggt tcagtatcta cattgaatcc atactctttt ggtgtatctc taacaataat
    27961 atacataaaa catccaatta caacaccaat tataccgatt aataaaaatg aatttctcca
    28021 accaacaagt tcagacaaaa acacaagtgg aaaagtagca agaactccac ctaaatttcc
    28081 tacaaatgaa aatttagctg ttgcagaagc aaattctgac ttattaaacc atctcccttg
    28141 aatttttaaa atacaaagta atattactga tgttcctgcc cctacaatta cacgagaaat
    28201 atatgctaat tctatacttt gaatcaaacc aaataaaatt gaccctaaac ttgccattat
    28261 tattccaaga ctgctaattt ttcttgctcc atatttatca atcaaaattc ctgcaggtat
    28321 ttgcataaat gcatatgcat ataggcaaaa ggatgctata tttgaaattt gtatagaatt
    28381 aaaacctaac tcatttgcta aattgtctga aacaacagca gttgacatcc ttaaaaataa
    28441 tactaaaaca aatgaaaatg taattatacc ccaaacactc cacttagcct ttgtataact
    28501 tttcatatct tctccctcaa atccatatat ttttaaattt tcttgatata acttatataa
    28561 agtgtatagt tattcttaca aatttttaca cgcaatatca ttatacaaca tatgtatata
    28621 tttaactaga aataaaacac tattcatatt atatacatga attaactaca taaataataa
    28681 gagccatctt ttaacattac tcaaaagata gctccatata aaaacattta ttctaaattt
    28741 aattttttat ctaatataaa ttttgtagcg aaaaataacg ctattgcaac tacaagacta
    28801 ccaagtattg caagcataaa actaggactc tgataaagat gatataccat atttgtatct
    28861 tcatttggta agatgcttcc aactgcatct ccaacaattg atattactat atttatagct
    28921 ataaaagaag caaatgccac tatgtttcta tgtttattga attttggcaa ttgtcccatt
    28981 gagattgatg tatataataa caaaataaat gatgaatatg aaataaacat tactactact
    29041 aataataaaa tatctaaaaa gttttcagaa atccatttaa aactagtgtt aaacaaatct
    29101 aaaatttcag gtagtaaaat tccagaagta ccaaaaagca taagaactcc aaaagtgaat
    29161 actgcaacta taaaactaag tacagcgtat ataagtgctg ttatacattt agataatata
    29221 agtgaactag tgctaacagg aagagtaaac attaaataac cttcatcccc caaaagattt
    29281 tttctaaacc tttgaattgt aactacaata gttaaaactc ctaatgccat aaaaagactt
    29341 gttaatacta gtattcctat tccctgtacc tgaagtatat ttgtattcat aaatattcca
    29401 ttaaatacag ctactatcaa tatggctata tataacggta taaatattct tccacttgct
    29461 tttaactcat attttaataa tttacctaac atttaaattc ctccctaaat aatgcatcta
    29521 tagattttcc tttttcttct cttattgctt caacgtctcc ttgtaacact atttctcctt
    29581 ttgatatgaa tattatctca tcacatatgt tttctatttc acttattaag tgggtagcaa
    29641 tcagtaaagt actatcttca gaataatttt ttaatatagt cttaagtata tatgaccttg
    29701 cagctgggtc aacaccacct ataggttcat ccaatatata tatgctcgca tttcttgaca
    29761 ttactaaaat aagttgaact ttttctttag ttcctttaga cattgtcttt agcttttcat
    29821 ttacatcaat atctaagctt ttaatcatct catttgctct tctaacatca aaatcactat
    29881 aaaaatcata gaaaaactta agtaaatctg ataccttcat ccaatcattt aaataagttc
    29941 tttctggtaa atatgaaact atttttttag tatctatgga tggtttcatt ccatttatca
    30001 taatttcacc attatcaggc tgtaataatc cattcattag cttaatcatt gtacttttgc
    30061 cacttccatt aggtcctaac agcccgacta tttttccctt tggtatattt aaatcaatat
    30121 tttttaaaac attcttagtt ccataactct tgtttacacc tttaaattca actattccat
    30181 cttgagaaac attgcttata tttttatcca ttttataatt ctcctttcag attttctgta
    30241 ataagtttta gcatttcatc tctatcatat ccaagctttt ctaaggttac ttttaatgta
    30301 tttatctcca cattagctat ttcctctttc atagctttta ttttttcctt atcatctgtt
    30361 acaaatcttc ctttagttct ctgactataa actaaacctt gtctttcaag ttctgttaga
    30421 gccttttgca ttgtatttgg attaacttcc gcatcttctg ctagtgctct cactgtttct
    30481 aatttagatc ctattttaat ttcccctgaa attattttta actttaaatg ttctactaat
    30541 tgtatatata taggtttgtt attatcaagt tcccattcca tgttcatgcc tccttattat
    30601 attattgtat taagttgtta atacaataat ataataatac aaagtatatg tcaattactt
    30661 tttagtaaaa gtataaaaaa ttttacaaaa aaaatgaaca ctacattaga gtccattttt
    30721 aacacaattt attaaattct ttatattaca gattaattac cctcttacca agttagatac
    30781 aattaaatgt tcaaattctt caattggcat tggctttcca aacaaatatc cttgtgccat
    30841 atcacacttt acttctttta aaagtttaaa ctgttcttct gtctcaactc cttctgatat
    30901 gactttcata cccaactctt ttgccatttc tattacttta gtaacaatta ttttgcttct
    30961 tttactatta taagtttcca taataaattt ttggtcaagt tttaagatat ccataggaat
    31021 ttcttttaac atatttaatg atgagtaccc tgaaccaaaa tcatccatag atattaaaaa
    31081 tcctattttt tttaaattat tcattatatc aattaaaata tcaaaattat caaatatgat
    31141 actctccgtt aattcaagtt ctatatactt tgcaggtata ttgtatttat caattatatt
    31201 tttgtatgtt tctataaaat tattgcaata taaatgtatc cttgaaacat taactgaaac
    31261 agtaactgga tttattcctg catcaatcca ttttctaagg tatatacata cttcttcaag
    31321 cacatataaa tctaaattta taacaaaacc gtttctttca aaaaatggga taaattcatt
    31381 tggaggtatt agccctttat caggacgttt ccacctaaca agtgcttctg aaccttgtat
    31441 ctctcctgta ttaagttcta tcttaggttg taagtatata ataaattctt tattttctag
    31501 acttgaaaac attgtatttt ccatctcttt ttcttttatc tcttggtcat gaatcttatt
    31561 atcataaaat gcatatgaac ttttatgtcc tccctttgcc attttatgag gtatattagc
    31621 cctatctatg attgagttga tatctctatc ttctggaagt ataaaataga ttccacaact
    31681 taaaactaac ttatattttt cttcttgatt attattaaac aagtctaatt cttcaaatat
    31741 agtttttaac ctctttgtta tatcttcttc ttctatatat ttcataatta taataaaatt
    31801 atccgcagat attcttgcaa acatttcctc ttcaaatata ttgctcttaa taatctttgt
    31861 taaattacgc aaaatttcat tcccaacttc atatccaaat aaatcattta tatatttaaa
    31921 tttatcaata tctatatata ataaagcata ctttacttga gtatttttac atagtatttt
    31981 gttagcatca attacaaatt tatctatagt atttgctcct gtaagattat ctgtatatgc
    32041 tatcctttgc aatttaattt taaaatttaa tttatcatat attatataga atataagtat
    32101 tacacatatt atagccataa taagtattat ttgtattgca tgtttgctaa taagctgatt
    32161 attagacaca atagactcaa agttatttat ggagttgttt ttatagagtt tgcataaaga
    32221 atagactggt gttgtgtatg taatttgtaa aattatacaa ataaataaaa aaaacatcaa
    32281 ttttttttgt aaattcctca taaaaatttc ctcccattaa aacacctcta ttttattagc
    32341 aaataagtac atctcaaaag tttccatttc ttgtaaaatg gatagtttat atctacttta
//
LOCUS       DAMJYC010000040        31175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJYC010000040 DAMJYC010000000
VERSION     DAMJYC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.041"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(21..1406)
                     /locus_tag="PC472_002008"
     CDS             complement(21..1406)
                     /locus_tag="PC472_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2727334.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(1514..2752)
                     /locus_tag="PC472_002009"
     CDS             complement(1514..2752)
                     /locus_tag="PC472_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2727335.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYNFKFNYFLCLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISTICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKTLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRNLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(2765..3133)
                     /locus_tag="PC472_002010"
     CDS             complement(2765..3133)
                     /locus_tag="PC472_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2727336.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLDC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(3661..4083)
                     /locus_tag="PC472_002011"
     CDS             complement(3661..4083)
                     /locus_tag="PC472_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2727337.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALIRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            4561..7464
                     /locus_tag="PC472_002012"
     CDS             4561..7464
                     /locus_tag="PC472_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2727338.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            7497..7943
                     /locus_tag="PC472_002013"
     CDS             7497..7943
                     /locus_tag="PC472_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2727339.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            8066..8368
                     /locus_tag="PC472_002014"
     CDS             8066..8368
                     /locus_tag="PC472_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727340.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            8549..9955
                     /locus_tag="PC472_002015"
     CDS             8549..9955
                     /locus_tag="PC472_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2727341.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            10299..11357
                     /locus_tag="PC472_002016"
     CDS             10299..11357
                     /locus_tag="PC472_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2727342.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCRKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            11456..12142
                     /gene="araD"
                     /locus_tag="PC472_002017"
     CDS             11456..12142
                     /gene="araD"
                     /locus_tag="PC472_002017"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2727343.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            12160..12807
                     /locus_tag="PC472_002018"
     CDS             12160..12807
                     /locus_tag="PC472_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2727344.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPILPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(12980..14347)
                     /locus_tag="PC472_002019"
     CDS             complement(12980..14347)
                     /locus_tag="PC472_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2727345.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(14537..14625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15053..17173
                     /locus_tag="PC472_002020"
     CDS             15053..17173
                     /locus_tag="PC472_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2727346.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            17277..18530
                     /locus_tag="PC472_002021"
     CDS             17277..18530
                     /locus_tag="PC472_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2727347.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDSTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(18684..19745)
                     /gene="ytvI"
                     /locus_tag="PC472_002022"
     CDS             complement(18684..19745)
                     /gene="ytvI"
                     /locus_tag="PC472_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2727348.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            20019..20759
                     /locus_tag="PC472_002023"
     CDS             20019..20759
                     /locus_tag="PC472_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2727349.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            20771..22549
                     /locus_tag="PC472_002024"
     CDS             20771..22549
                     /locus_tag="PC472_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2727350.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            22716..23633
                     /gene="pfkB"
                     /locus_tag="PC472_002025"
     CDS             22716..23633
                     /gene="pfkB"
                     /locus_tag="PC472_002025"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2727351.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            23670..25589
                     /locus_tag="PC472_002026"
     CDS             23670..25589
                     /locus_tag="PC472_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2727352.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            26185..27711
                     /locus_tag="PC472_002027"
     CDS             26185..27711
                     /locus_tag="PC472_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2727353.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            27739..28296
                     /locus_tag="PC472_002028"
     CDS             27739..28296
                     /locus_tag="PC472_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727354.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            28818..29861
                     /locus_tag="PC472_002029"
     CDS             28818..29861
                     /locus_tag="PC472_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727355.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VEGEKIDLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            29972..31114
                     /locus_tag="PC472_002030"
     CDS             29972..31114
                     /locus_tag="PC472_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2727356.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYIFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT        12356 a         3332 c         5192 g        10295 t
ORIGIN      
        1 aaatattgtg tgcaatacaa ttattttttc tcttctaata gtctttttaa tacgctcaaa
       61 ccagctttaa gttcttctgt actttctgta gcacatacag ctattcttat tgcagaaatt
      121 ggtttttctt ttccaacagc aaaacgttca gaagcataga cctgtacacc ttctttaagt
      181 gcttgtatct caaatttttc tgccattatt ccatcaggta gaatcaacca tctaaatata
      241 cattcttcat ttcccaaaag attatatcca gataaatatt gattaataat cttgtttctt
      301 cttcttgcag atttacggcg tgcattaatt aatttatcgg cttcacctga tgtaatcatg
      361 cgataagcaa tttctgttaa aaaataagat tgagatagat taatgttgta taatgcgctt
      421 gataatgcct ctcgatactg tttaggtgta gagatatatg ctagccgtaa actaggtgca
      481 ataatttttg ataaactcgt aatataaatt gtttgattgg gcagatagga agccactgga
      541 tttaaatgtg tttcattaag taaactatga atagcatcct caataacaat taaattgtat
      601 ttagatgcaa tatttgcaat catttttcgt ccattttgtg acattatatg tgtagttgga
      661 ttttgataat ctggtattat gtatagacct ttaatatttt catttttaca agcatataat
      721 agtccttctt cactaatctc attatcttct tgtttaattg gaattagttg aatcccaagc
      781 attttagctg ctgtctttat tccaggataa gttaatgggt ctacacctat tctatctcca
      841 tgttgaaaca aaccagctaa aatggcaaca attgcatttt gtccaccact tgcaggaagt
      901 aagctatctg atgtagtttc aagcccagct ttataaatta atttggcaat agcttctttt
      961 tgccatttag caccatctct ttgtccatat tggaatatgg ttttaaaatc tatttcctta
     1021 attatgtgtt taaacaaagt attcatttct tctagcgtga aatctggatt cattgtgccc
     1081 atttcaataa atgtaatttt attattactg gacatcaaga atagatttga acgtgtatca
     1141 taagatacaa aagttccact tcctgtaact ccacttatta gacctttttt ttcacaaatt
     1201 ttaaaagctc tagagatagt actcacatta acatctaaga aatctgccaa ttcacgttgg
     1261 ggtggtaatt tagtacctgg taataaaaat ccattgttga tatcttgttc tagttgttct
     1321 gcaagagcct tatataatat ttgacctttt gaaggtctct ttggtttcca gttcatagga
     1381 taattttcaa aagaattaat tggcataagt tttcacctca cattattaat ttgatttttt
     1441 ctttatctaa aacaataaat tctaaagata caaataaaaa ctgataggaa ataattctat
     1501 cagtttttgt cttttaattg atttcaaatc ttcctataat ttttatacct tctttatcat
     1561 aaagagggat tgttttttta aatatattaa aatttctttt tcttgtatat aaaacagctt
     1621 cttctggtgt ctttggttga ttatcacaat cgtacaaatt tctttttttt acgtagccga
     1681 cttttttacc tctacctatt gtttgtataa gttctggttc aatggaattt ccatttgagt
     1741 ctattatatg tgtaccatag gtttctccat ttttattata attaatctta ccaatagtat
     1801 attctacatc ttcattttgt ttaaaattgg agttgataga aaatattgac cctattatta
     1861 taaaggttaa taaaaactta agacctaaat taattttctt tttatcttga ttaaacatag
     1921 aatctaacct cttttttaaa ataatatttt ttttatttag aaactgagat acgaatgatg
     1981 aatacttcaa agttttttga tactttattg tatctaatat tatatatgca tactctttaa
     2041 tttcactcgt ggtacacttg tcaatcacaa tttcatcaca agataattca cataattcat
     2101 gaaaatattt ttttaataag tataaaattg gattgaacca atatatgata caaattgtac
     2161 taattagaag cttaaataag ttatcttttc ttttataatg aattaattca tgtttaaaga
     2221 tgaatcttaa ctctttatta ttgtactttt tttctggaat tattatttta cagcgtgtta
     2281 aacctataat ggcaggagaa taaatacctt taaccttata tatttgaagt tttttattta
     2341 tacttagttc ttttgcttgt tcatctaaga tactagtaat atctatttct aaaatttctt
     2401 ttttaaaatt ttctatgtaa ttataaaatt taaaatttgt tcttaaatag taaaaagaga
     2461 ttgctataat tcctaatatc cagatgtaca taaatatatc taaagtaatg ccctggtcat
     2521 atggtatata attatcgata ttatgtttat agatattaat agtagtatta ttgatattta
     2581 atatatttaa atttatttta gatataaaca tcattctgac tactattaaa agacataaaa
     2641 aataattaaa cttaaaatta tatttgctta tctttttagt aaataaaatg aaaaaaagaa
     2701 ttagtggact tatgtaaata ttaaatttaa taacttctat aaataattcc ataaaatacg
     2761 tctcctattt tctttttttt agccattgtt caagttcttt tatgtcatca tctgatattt
     2821 catttccaca atctaatgat gatataaagc tatgaataga atttttatgt ataagctcta
     2881 aaaaatcctt tgtttcaaat tttagatatt cctcatgttt aactttaata gtgtataaag
     2941 tatttctacc atctttttca aattctaaaa aacctttatc acttaatcta gaaagtactt
     3001 taccagtagt accttttgtc catccatgag aaatttccat ataattagct acttctctgg
     3061 ctggtatttt atctatgcct ttatcccata tgaaattcat aactaacaac tcggattttg
     3121 gtattttttt caatataatc actcaccttt aacttgatta tattagacaa atgtctaata
     3181 gtcaatataa tatatcataa tgtataaata atctttatat tattttatgc ttgagaacta
     3241 agtagtatag atttatagat aaaaaagatg ttgagagttt tatggaagag gggaatatat
     3301 tttctattag agataagaat atgacatata ttttttattg atttgtacaa aaatactata
     3361 tagtgaataa attaatatct tttctaaatt agaagaggat aatcttatta gataaagatt
     3421 atcctcttta ttgaaagttt aagggatatt agagattatt aatttttctc tcttttttta
     3481 actaaaagca ataatacata aaattattat tgctaatgat actcttaatg tggtatatag
     3541 tcatagtgtc acatgtttat caatagtttt ttcagtaatt attgtaacga atagtaggac
     3601 tcataaaata cttagaaata tgtttttaga taaattttaa atatataatc taacttatat
     3661 ctataaatta acactattat tctccaattc catttctagt ttttttataa atttattaaa
     3721 ttcttctggt tgcctttttc caaacaatac tcttataaga gctgaattga tttttccaaa
     3781 tatattcctc ttgctctcac tttcaataaa aataagtttt gtattttcta agtcaatatg
     3841 tactaattca tatctagtaa tagtactttc tcttttattg cttgttttta tctcatatac
     3901 tctatttttt tcgaaattag ttatttcaac cttaaatctt ttattatttt tttgctgtac
     3961 aaatgttcct actgcttttt tctcattaaa attaggaaaa tttagtcttg cagattttgt
     4021 aaatatatta aatatgtctt ttgcagaata ttttaataaa acttcagttt taaatgtatt
     4081 catatcgttt tcccttctgt atataaattt aatatttata agttaatttt taagttaaaa
     4141 tagtataata tgccatatta aatcatgtta aaactattta tatagctaaa atcttaccta
     4201 tataagtatg tatactatca tataaaaata aaatcttaaa atagaaaggg ttaaaaacac
     4261 agtttattat aatttttatt gttgagtaga tacgtactat tatatatgat tgagcaagtt
     4321 tttcaatcat aaatttataa agtattttgc attaaaattt aaacaaagaa ttaaattatt
     4381 taagattatg aataactaat acatttagta aattactaaa tatacaaaat gtaaatactt
     4441 atcaaaatgt ataaatactt atgacatcta gttacttaca gggtttaagt agaaaaaaat
     4501 atcaaagtaa gtatggcatg atagttgctt atatatttat caagaaagga gttattaagc
     4561 atggagttga gagataaact tgtaaacatc ataaatacag aaaataaaaa agatccatta
     4621 acagatgttc aaatagcaaa gtttttaagt acaactagag agaacataac taatttgaga
     4681 aaagaactta atataagtaa ttcaaggcaa agaagatatc cttatttaaa atcagcaatt
     4741 tcggcaatat tacaaaagaa taagaatatt tctatatcag aaattacaag ggaattgatg
     4801 acagaaggct ttaatatatc tagaagagtt gtggaggaac ttttacctaa agaaagttta
     4861 gaaatggatg tagtagagga atctgaagaa gaatcgaatg acccatttga aactctgata
     4921 ggtaataaag ggagtcttag aaatgctgta gagcaggcta aatctgctat attgtatcca
     4981 ccaaaagggt taccaacgtt gattattggg gaaagtggag ttggtaagag tttattttct
     5041 agacatatgt atgagtttgc aaagcaaaaa aagatagtaa aagaaagctc caattttgtt
     5101 gtatttaact gtgctgatta tagtgacaat ccacaattat tattatcttt gttatttgga
     5161 tataaaaaag gtgcatttac aggtgctgaa tatgatacac caggtcttgt agaagaagct
     5221 gatgaaggag ttttattttt ggatgaaata catagattgc ctccaaaagg ccaagaaata
     5281 ctattttcta ttttagatag gggaaagttt aggagattag gtgaaactaa tgctgaaaga
     5341 aaagtaagta ttatgttgat tggggcaact actgaaaatg tagagactaa tttattatta
     5401 acattcagaa gaaggatacc aatgctaata actttgccac cattacatga tagattacta
     5461 aaagagaaaa tagaccttat atacaatatc tttcaacaag agtgtaatag aataaatgca
     5521 aaaatatttg tagataaaaa tgtgattgaa atacttgcac taaaaaagtt tagtggaaat
     5581 ataggacaat tacaaaatat gattcaggtt ctttgtgcaa gagcttttat gaagtttata
     5641 aattccaaag atgaagatag tgaatcgata gtagtagtag atataaatga agttttaaag
     5701 cttaaagatt cttttaaaga tgctgccttt caagagattg aatatacaga gataagaaaa
     5761 tatttgaaaa atgctatatt tataccattt aatttagaag aatcatcagt aaaaggaagt
     5821 cttttaagtg atgaatatat attaccagag gacatataca aaacaattga aaagaaatat
     5881 tatgacctaa aatccctaga cattgaagat atagatatag agaatatttt atggactttt
     5941 attttaaata gatttgctga cttaaagttt aatctcgata gtaatgaaga aattttttca
     6001 atgagtgatt taaatagttt tgtaagtgat agtttagtta aactggtaaa agaacttatg
     6061 aatgatatag ttgaaaaaaa tatgaattta gaagtaaata aaaatatgtt caaatatcta
     6121 gcaatacact tagaagaagc tataaaaaga attaagctaa atcaaaagat aataaatgta
     6181 aatttagaaa aaataaagat tgacttttca aaagaatatg aaattagtaa aagttttgca
     6241 ataaaaattg aggaatcaat tggtattaag attccagatg atgaaattgg atttataaca
     6301 ctctacataa agtctgctct aaaaaatgaa gtgaaaaaag ataaagtagg gttaataata
     6361 atttctcatg gacgtatagc tacagagact gtaaatgtag ttaaagaact tttaggtgta
     6421 aagtttcctg tggctataga tatgccttta gatgaaaaac caataaatat atacaataag
     6481 gcagtagaac tgtcaaaaat aatagaccaa gggaaaggca tattgttttt ggtggatatt
     6541 ggttcactta caaacatagg tcaaatagta aataagagaa ccaatataag cactaaaact
     6601 attgatagag tagacctcct aatggcacta gaagcaacta gaaaagtttc tatgggagag
     6661 gaagaacttg atgaaatatt tttttctttg ctaaaagata ggatgggata taactatgaa
     6721 ttagaaaaac acatagataa atcaaatgca attataacat tatgtcttac tggagaagga
     6781 actgctaagt atataagtaa aacgttggaa gaaaagtatg ataacactaa atgttatcaa
     6841 atgagtgcat tggatgaaaa tttattttgt aaaattgaaa atctaaaaga gacaaacaat
     6901 atattagcta ttgttgggac aataaaccct aagataccag gtataaattt tataccctat
     6961 aataaagaac ttttcaaaaa tttggatatc tatctatcaa aatcaaaaca acaagatgac
     7021 agaccaatta aatcgtatga aagaatgctt gatgaggatt tagtgatttt tgaaccagat
     7081 atatatttta aaaaggattt attagaatat gtatgttcaa ttcttataaa taaagaatat
     7141 gtagaaaaag attacttaga ttctgtttta catagagaag aaatgcttcc aacatattct
     7201 aaaggagcta taggtgtgcc tcatggagat tcttctactg taaatagtac aaggtttgtt
     7261 tttgttaaac taaagaatcc tattgattgg ggtgtaggta atgtaaattt catatttatg
     7321 ccagtttttc aggctaatga taaagagatt gttaaaaatg ttcttgagat actaaaagat
     7381 gcagaattta tgaacaatgc caataagtgt tttgataaag atgcttttag aaggattata
     7441 tttgataaat ttaaacatct gtagccatag caaaaaaatt gagaggagtt agagtaatga
     7501 atgtagattt aatatcattg ggtttagaat ctaatgatta tgaaagtgtt attgaagaat
     7561 taggaagtat aatgtgtaaa aaagaatatg taaaagaaac atacataaat gctgtcttgg
     7621 aaagagaaag gacattacca acaggtctag atattggaga aatgtgtgtt gctattccac
     7681 atacagattc aaagcatgta aatgagtcta atgtagctgt agggatattg aaaaatcctg
     7741 taaagtttaa ttcaatgata gacccaaaag atagacttga tgtagaactt gtatttttat
     7801 tagcagtaaa gaacccagat tcacaagtaa aactgttaaa ggatttaatg tcagtttttc
     7861 aaaatataaa gttattaaaa aatataaaaa acgcatcaac aaaagaagaa gtagctaaat
     7921 tattggactt tatagaaatt taatgtagga gaattttata aataaaaaat taaaatttaa
     7981 acaatcaaag taagtattat ttttttaata ggtttaatct tatataaaat aaaaatttag
     8041 agtgaaaaat taggaggaaa ttattatgaa acaatttaat gtattatcag tttgtggttc
     8101 agggacagtt acatcatcta tggtagcagg taaattaaaa gaagtactag gtaataaggg
     8161 gatttctatt actacaacag aagctagacc aacagaagca ctaaatttag cacaatcagg
     8221 aagatttgac tttataacat ttacgagtcc acttcaaaca ggcgattatg gaataccaac
     8281 tataaatgca tttgcttgcc taactggtat aggggaagag gcattctttg aagaagtttt
     8341 gactgttatt gaagggatag ataagtaagt tttggagacg tgaatttata gtatttaaat
     8401 caatgattta gatactataa taagctaata ttaactaaat atttgtggaa aaaacatttt
     8461 atatgttgtc ataaattaaa aaattataaa aatagtcact taagtattaa attaagctta
     8521 taaatattta taagtaggag ggaaaaagat gttagaattt atgaaatcta taatcgatac
     8581 atttggttct gcaattatag ttccaattat aatattcata atagcaaaga tatttaaagt
     8641 aactactaag aaatcgtttc tttctgctgt gtatgcagga gttgctttac aaggttttac
     8701 actgatttta aattcattta caccaataat aacaccagtt atcaatagga tggttgagag
     8761 tacaggagta aacctaccag tttttgatgt tggttggcaa gcaacatcat tggttgcatt
     8821 ttcaacaagt gcaggtatga tatatttagg tcttggtata ttattacaaa caattttatt
     8881 tttgataaaa tggacagacg tgtttcaacc atcagattta tggaataact attcttatat
     8941 ggtatgggga gcaatggtta taggagtaac aggaaatttt cctttaggta tagcatgtat
     9001 ggttttatta aacctatata gtttattaat atcagagtta gttgcaaaga gatggtctag
     9061 ttactataga tatcctaatt gtacaataat agctatgcat aatgttgaag cttctgtgtt
     9121 tgcagtattt gcagacccta tatacaataa attgggatta aataaaataa aattaaatcc
     9181 aaaagaatta gagaagaaat tagggttttt aggtgaacca attactttag gtcttttttt
     9241 aggtatgttt ataggtatat taggtaatat gacgagaata aatactatgg aagcttgggg
     9301 agaaataatg aaagtaggta tatctacaag tgcagtgatg gcaattttcc ctaaagtagc
     9361 aagtatgttt gctcaagcat ttgcacctat aacagaagca gctagaaaga ttatgcaaaa
     9421 agctggaaac cgtgagtggt acatagcagt aaatgatgct gttggatatg gtgaacctgc
     9481 aactttaata tcaggtctta tattaattcc aataatgctt gtaatagcta tggtacttcc
     9541 tggtaataag gtattaccag ttgtggattt attggcaatt ccatatatgg ttcaaggttt
     9601 agttgccata cataatggta atataccaaa agtattagtt tcaggtataa tttggtttgg
     9661 attaggatta tatgtttgta catctacagc accattattt acagatatgg ctacaaatat
     9721 aggtgtagct atacctgcag gagcaatgtt aataacaagc tttaatatac taggaaaacc
     9781 tttgatggga ttagttttct ttgcatttct aagtgcaaat ccaatttata taggtctttc
     9841 agttgtaatt tactttgttc tatgggcatt gtttagaaag aacaaaacaa gtattcttga
     9901 ttatttagaa aaacaagcac tcaaaaatgt agaagaagag cctgtagcag tttaagttta
     9961 taaaaatgaa ataagaataa ttaaaggagt ttataattga ttttatataa gtatcagata
    10021 atacctgaga aatatttaaa tgaaatatag acttcttttt atttgataat atttgtaatt
    10081 ataatgcatt gtatttttat agttgagttt tctgtataac ctgctattta aaacatactc
    10141 ttttatattt atacagtaat gtggtttaat tttataaaat gtaatctcta ctggtaaaag
    10201 actttgtata taaggcattt gcctgaataa accatttatc ttttgtatgg catagatatt
    10261 gctatataga agtatatagc atgaaaggag atatttatat gggagataaa atgagagcat
    10321 cagtattgta taatgtaggt gatgtcagat atgaaatggt agatatacca gaaataacag
    10381 atacgcaagt gcttgtaaat gtaaaatatg ttgggatatg tggttcagat ttacctaggt
    10441 ctatggtaag tggtttgagt ggaaatacaa aatatccatt gattttaggg catgaatttt
    10501 ctggtgaagt tgttaaaatt ggagaaaaag taaagcatat taatgtagga gatagagtag
    10561 ctgttgcacc attagtacct tgtagaaaat gtgattattg taatgaaggt aattttggct
    10621 tgtgtgatga ttacaatata attggaacaa gagtaaatgg agcgtttgca gaatatgtaa
    10681 gagttccaga agaacatatt ttaaaactac cagatacatt ggattatgaa actgctgccg
    10741 gaattgaacc agcaactata gcttatcatg ggatttcaaa gtctaatatt agagtaggag
    10801 atagtgtagt tgtgttagga tgtggtccaa taggtcagtt tgttatacaa tgggcaaagg
    10861 tgtttggtgc atctaagatt atagcagttg atatttttga tgaaaaatta gaactttcta
    10921 aacttctagg agcaaactat atacttaact caaaagaagt aaatgtaatc aaagaaataa
    10981 aaaaaataac aaatggtggt gctgatgttg taatagagac tgctggtagc cgatttacac
    11041 aagaacaatc tctatttgta gcaaaaaaga gaggaaatat agtgtttgta ggtatatcac
    11101 acacagaatt accacttagt gcagacgcta ctgagtgtat attacgtgga gaacttacct
    11161 tgaaaggttc ttggaattca tatacaagcc cttatcctgg aagagcatgg actgcaacat
    11221 tggattttat gggaaaaggt gacattattt ttaaacccat gatttcacat aaaataggtc
    11281 ttaatgaagt tggtgacttt ttaagtaaaa tgagcaagag agaaattaat ttcaataaaa
    11341 tcctagtaga aatataaaaa tttaataaaa aattctaaaa gcaattaaat tttataacta
    11401 tgacattaaa taaaataaaa ataattttct taaataaaat ataggaggaa gaaaagtgtt
    11461 agaaaattta aaagaagaag tattaaaagc aaacttagaa ttacctaaga aaaagctaat
    11521 tacttatact tggggaaatg taagtggaat agatagagaa aaaggattag tggtgataaa
    11581 gccaagtggt gtagagtata gtaatatgac tgtagaagat atggttgtag ttgatttaga
    11641 gggaaatata gttgatggca aattaagacc ttcttcagat accccgactc atctagtttt
    11701 atataaagag tttgaagaat tggggggaat agtacataca cattcaagtt gggcaacaat
    11761 ttgggctcaa ataggaaaat ctataccatc ttgtggtact actcatgcag attattttta
    11821 tggaagtata ccatgtacca gaaaaatgac tagagaagaa atttgcaatg aatacgaaaa
    11881 ggaaactgga aatgtaatca ttgaagaatt tatgggaaga aatccaattc attgtccagg
    11941 aataattgta aatgaccacg gaccttttac atggggaaaa gatgcaaatg aggctgtaca
    12001 caatgcagta gttttagaag aagtcgcaaa gatggcttat tatacagagt taatgagtcc
    12061 tgataatatt atggataagg ttcttatgaa taagcatttt tcaagaaaac atggaaaaaa
    12121 tgcatactat ggtcaaaaat gattatctgg aggtgaatta tgctagactc tttaaaaggt
    12181 aaaattgtgg aaatagcaat taaagcagag cattatggtt tatgtaaaga aaagactggt
    12241 aattttagta ttagagataa ggatactgga tatatactta taaccccatc aggtatagga
    12301 agagaagaat taaaagttga acatatttgt gtattggatt tggatggaaa tgtaatagaa
    12361 gttgagaaag gtattaagcc aagtagtgag gtattgatgc atattgaaat atacaaaaca
    12421 agagaaaata taaatgccat atcacatact cattctttat atgcaacagc attttcagta
    12481 gcaaagaaac caattctacc aattgtatat gaatcagtta attatggcgg ttatgtagat
    12541 attgctccat acgaaagacc aggaacttta aaattagcta agagtgtttc aaaattactg
    12601 atagatacag atgcatgctt attagaaaga catgggttga caactgtagg taatgattta
    12661 gacgaagcat tattaaaatc aagatatgtt gaagaggtag cagaaatata ctataggtct
    12721 ttagtattaa atcaatttaa agaacttgaa gtagtagatg ttgatgagtt taaatcttgg
    12781 aaatacccag aagaaataaa gttttaatct aaataatttt cattgaagtg gttaattaaa
    12841 atatatgtat tatttatatc aattactatg ttttattata ttaattaccg tattttgtaa
    12901 atatatttat tacatttttt caaaaaaata tagaatccga aagccatata aaattgactt
    12961 gggattctat actttttatt tatgcatttt tctttatctg attttctgga gaatttatat
    13021 gttcaaaata ttttttagtt tcagatacta ttataggtga taatgcaagt aaagctataa
    13081 ggttaggtat tgccataagc ccatttacaa tatcagcaat tatccaaaca acttctaact
    13141 ttaagaaagc acctaataac accatagcca caaatacagt tctataccca ttcatgcctt
    13201 ttacaccaaa cagaaattca aaacatcttt ctccataata actccatccc aaaattgtag
    13261 taaatgcaaa taatgttaaa ctaactgtaa gtaatattgg tccaaatgta ggaattgctc
    13321 cgttaaaagc tgcttgtgtc ataacagctc catttaggtc tgaacaccac actcctgaaa
    13381 taacaagaga gaatccagta agagtacaga ttataatagt atctataaaa gtacctgtca
    13441 tagatataag accttgttcc gctggccatt cagttttagc agcagcagca gctattggag
    13501 cactacctag accagactca tttgaaaata caccacgagc gataccattt ctaattgcca
    13561 gtgacatagt tgctcctaaa aaaccaccag tagcagcagt aggtgtaaat gcacttttaa
    13621 ttattaacat aaaagtttca gggattttat tccaaaaaga tattaaaatg attccacaga
    13681 ttacaacata tataactgcc ataaaaggta ctattttagt agctacatta gatatacttt
    13741 taagtcctcc aaatacaacc aaagctacaa atactgctaa tacaacccct gtaattctag
    13801 ggtctatccc gaaactatta tttattgcat ctgttattga atttacttga gtaaaagtac
    13861 caattcctaa aagtgcaact agtataccac ttatagcgaa aaatattgca agaggtctcc
    13921 atttttctcc cataccattt actatgtaat acattggccc accagacact tggccatttt
    13981 tatctttagt tctgtatttt attgcaagaa caccttctga atacttagta gccattccaa
    14041 agaatgctgc tatccacatc caaaaaagtg caccaggtcc accagctttg atagctgtag
    14101 caacaccaac aatattacca gtaccaactg tagcagctag agcagtacat aaggctgcaa
    14161 aacttgaaac atcaccacta cctttgtttt cagcagagaa tattaactta agagctcttg
    14221 gcagtttagt tatttgaaca actccaagct taacagtaag tagtatacca gtaccaacta
    14281 atagtaccag taggggtggt ccccaaataa aagcatcaac tttgtttaaa atatcaatta
    14341 gattcataaa aacactccct catttttttt gctttacata atcaatagaa tttttaatga
    14401 agtaattgac aggaaataag tataatttat taataatttc tagtgtaata aaaaaagcta
    14461 aatctaaaaa agattcagct ctggatgtac actaattagc aaccaaaata aaagtattag
    14521 taaatactgt ttttggtaga taatccctgt ccttttgcct gagagattga gcataaaagc
    14581 cttgcccctt cggcgcccat aatgggtctc tccagagttc cgtaaatttt acagtcctac
    14641 ttttaaaagt acctgagagt gttactcctt cggtgtgcta tatagcaact cttctataaa
    14701 atcttaaccg gatattgatt tgtcatagct tattgtatta ataatattaa tataagtttt
    14761 tatgttttgt caactaaaaa attaaaaaac aaagaaaaaa tataaaaatg taagcctatt
    14821 tacgaattat aaacttagaa agatgagtaa tatacgtgaa aatgaatttt gtaatacaca
    14881 tacaaatgaa gtgtaaaata agttttaaat aaaaaaataa taaaatctta aataattaat
    14941 tagatatgat taaattatgg taatattata gttacgatat taagaaaagt tctatttagt
    15001 tagattttaa tcgtaatatg agattatgta tagagaaagg atgtattttt agatgggaaa
    15061 aatattagtg ctagcagaaa aaccttctgt tggaagagat atagcaaaag ttttagggtc
    15121 aaaaaatgaa aaaaatggtt atatagaggg gccgaagtat gtgataactt gggcactagg
    15181 tcatttggtt acacttgctg accccgaaag ttatggagaa agatataaat catggagctt
    15241 agaagacctg ccaatacttc ccaagcacct taagactgtt gtaataaaaa agagtggaaa
    15301 gcagtttaat acagttaaat cgcaaatgaa tagaaatgat atagatgaaa tagttatagc
    15361 aacagatgca ggtcgtgaag gagaacttgt agcaaggtgg ataatagaaa aatcacaggt
    15421 aaaaaaacct ataaaacgtc tttggatttc ttcaagtaca gacaaagcaa taaaagacgg
    15481 atttgcaaaa ttaagaagtg gaaaagaata tgaaaatttg tattactcag caatagcacg
    15541 agcagaagct gattggatta ttggaataaa tgcaaccaga gctttgacaa caaagtacaa
    15601 tgcacagctt tcttgtggaa gagtacaaac tcctactctt gcaatgttgc ttaaaagaga
    15661 agaagaaata agaaatttca aacctaaaga gtattatgga ttagaattaa ttgctacaaa
    15721 gggaaattca gatataaaat ttatttggaa tgataaaaac aataattctt caacttttag
    15781 taaagaaaag atagaatcca cattaaaaaa agtaaaaggt gtagacataa aaatatctga
    15841 agtaaacaag tcatataaga aaaaatattc acctgcactc tatgatttaa cagaattaca
    15901 aagagatgca aataaaatgt ttggattttc agcaaaagaa actttatcta taatgcaaaa
    15961 actgtatgaa catcataaag tacttactta tcctagaact gattctagat atttaacatc
    16021 agatattgtt ggaacattaa aagatagaat aaaagctgta aatattagtg attattcaaa
    16081 gatttgtaat agattattaa aaagcaatat tatagctaat aaatcgtttg ttgacaattc
    16141 aaaggtaaca gaccaccatg caataatacc aacagaacag aaggtattca tgagtgattt
    16201 tagtgataaa gagcgaaaaa tatataatct tgtagtaaaa agatttttag cagtactatc
    16261 tcctcctttt gaatatgagc agacaactat aagtgcaact gtagaaggag aaatatttaa
    16321 ggctaaggga aacaaagtaa taaatttagg ttggaaagaa agttataaag atattgaaga
    16381 tgaagatgag tatgaaagta taccaaatat tttagaaaaa gatgtactta aggtcaaggg
    16441 attaaagata acttcaggaa aaacaaatcc accaccatac ttgaatgagg gaacattatt
    16501 gactgctatg gaaaagaatg gtctagggac agttgctaca agagcagata taatagaaaa
    16561 attattcaat tcatttttaa tggaaaagaa aaataaagat atatatataa catcaaaagg
    16621 aagacaatta ttagatttag ttccagtaga cttaaagaca ccagaactta cagcttcatg
    16681 ggaaaagaaa ttattggata tttcaaaggg aaaacttagt aaaaatacat tcataaatga
    16741 catgaaagat tattcaaaag atattgtact agaagtaaaa aatagtgata ataaatttaa
    16801 acatgataac ttaactaaaa atagatgtcc agaatgtgga aaatttatgt tagaggtaaa
    16861 tggtaaaaaa ggtaagatgt tgatttgtga agatagagag tgtaatacta gaaaaactat
    16921 atcacaagta accaattcaa gatgtcctaa ctgccataaa aaattagaac ttcgtggaga
    16981 aggtgagggg agaatcttca cttgtagttg cggatacaga gaaaagttgt cttctttcaa
    17041 caagagaaag caagaagaaa aacagaaggc atcaaaaaaa gatgttaatc aatatttaaa
    17101 aaatcaaaat aaaaatcaag agacatttaa taatccattt gcagaagcat tagctaagct
    17161 aaaaaacaaa taattttaaa gttattatta tacacaatta taactttaac tgtatactag
    17221 ttatatgtac tgatataata tagtgtatag tttaggcatg aaagtagggc tgttaaatgg
    17281 atttaagtgg catatttaaa tactattgta aagagtgtga aaatacatgg aataattcga
    17341 gtgttgaatt atttgagaat atagaaacgt atagtaaaga ttcacaaaaa aagagggaaa
    17401 aagaattaga taaattgcta aatacaatat cagttcattt agagaggtat ccaagtgatg
    17461 ctgtattgag aaaaatgtgg gtaaaaaagg gcgaggtttt tttacaaaag acattggaaa
    17521 aagaaaatat ttttaagtta gaaaaaatgg atgtagagga tagaaaaaaa tttttagata
    17581 taacaaaaca gtttattaga gatgctagaa aatttgatga tgatttacct ataggtgata
    17641 ttatgcaagc tatgaggaat gtatggattt caaatgcatt acaattatta tttggtaaag
    17701 aagtatatta ttcaaaagct aactttgcat atagtatgtt atatccatat actgataatt
    17761 atttagacag tacaaatata gataaaaatg ataagatttt atttaataac tggttagaaa
    17821 aaaggctcct gggagaacac attaaatcta aggattatca tgaaagtaaa gtatctcaaa
    17881 tgatagatta tattgaaagt gtatacccta gagaaaagtt tacagaagtt tatgaatcgt
    17941 tattattaat atttaaaagt caagtaaata gtttaaaaca acatggtaag gaaaatcatt
    18001 tgtgtaaaga agatttatta tccatttcta ttgaaaaagg aggttcatcc gttttagtag
    18061 atggatattt aataagtgga ttgatgacaa aggaagaaat agagttttgt ataggatatg
    18121 gatttttatt acaaatatct gatgatttac aagatataaa agaagattta aaatacaacc
    18181 ataaaactat tattacagag atgtcaaaag agggtacttt agataaagtt gtaaataaac
    18241 taataaattt tactattgag ttaatagata gttttaaaat taataataaa aataaatctg
    18301 taataactat gataaagaat gattgcttaa tgttaatttt attttctgta gtttataatg
    18361 ctgaattttt ttctgtagga tatataaaag aagtagagaa atttattcca tatacaatag
    18421 attattcatt agagattgaa gaaaaaataa aagaaaaatt taaaaatata gatgttttaa
    18481 ataatgaaaa tgaatataaa gaaatgattg atattatttg tgcagagtag aaaaaaaggg
    18541 agtgtcttag aatagacttt ttagtttatg aggcactctt tttgtataaa atcaaatgta
    18601 ttttcatcat tttaacaatt aaaaagaact tatccctatt tgagaaagcc ctttttaatt
    18661 taaataaaaa tgtcaaggtt aaactattta aatgcagtaa taatcccatt atcatgtaag
    18721 ttttttatga ttgtagctgc gatagggaat ccaaataatc cagctattcc aaatagttga
    18781 gccccaacaa acatacacat taatgttatt aaaggatata ggccaatttg gtgtcctact
    18841 acctttggtt ctaatatttg tctaacaacg gttataatta tgtatagtat taaaagtcct
    18901 attgctaaat taatatttcc attaatgagt gagataatca tccaaggtgt caaaatactt
    18961 cctgtaccca agactggcaa aatatcaaca agtgctatga tgacagatat agtaaatgca
    19021 ttttcaacct ttaggatagt aagtccaata agtagttcta tgaatgtcac tgatagtagt
    19081 attgcatagg cttttagtag ttttaaaaga gcattaatac tgctattttt tatatttatt
    19141 atcataaatt gacttttttc tggaaattgt tttaagataa agtttactat tttttgatag
    19201 tcaaaagtaa agaaaaaaga agcaattaaa gtaataataa gttttaacat aaatgcaggt
    19261 gttttacttg caatactagc aatagcattg agtgcatttg ttgaaataga agctactaaa
    19321 gacatcatag attgacttat attatcccaa ccaagtatta atgctaggtt aacattaggt
    19381 gtactagcat ctatctttga aaataaagta tttagcgttg gttgtatact tgatttatat
    19441 atttcaggaa gtctaaaaaa tacatcactt acacttgcaa atatcttcac accaaagcta
    19501 actaataaaa atatacacaa cccataaaaa attaaaagta ttactataga aataagtgta
    19561 cgatttaaat ttgtattatt tgtaataagt cttataatag gtcttaagat ggatgcaatg
    19621 ataaaactga ttacaaaagg cattaataat ggcaataaga attttaatac aatatataca
    19681 atacttgtta ttaaaactac atatgtaaat ttaattataa aattcttttg cttttgaata
    19741 tccataatat aaaacctcca atgtatttga tattattcta aatatggtct tttttacaga
    19801 aaatatacta ctgtatattg attataaact ataaagctta tgtaaagtaa ataaattatg
    19861 taaaatttag gttaattttg tataaagaga tacttttata ttttacaatt aataaccaaa
    19921 tactaaaaaa tattttaaat aaagaactta aaattgtgat aaaattaatt tatagatatt
    19981 aaaaaaagta taatatatgg tttttaagag gtgctaagat gaaaaatttt agagatttag
    20041 gtgggaacaa aactgaggat ggcagaactg taaagaaggg attattttat aggtctgcaa
    20101 aattatcaaa tctaagtgaa aatgatataa agattttaaa agagttaaat attaaatata
    20161 tatttgatta tagaagtgat gaagaagctc gtaaacaccc atcaacaata atatcaaata
    20221 taaaaaatat aagaatacca gcaatgagag agctagaaga atcaggagga agttttggtt
    20281 ctattgaaga tatgattgat ggattgtttg aaaaagatgg tgcatttaat atgttaaata
    20341 atagttacta taatttgcct ataaataatc cgtcatataa aaaattagta gaacttataa
    20401 gagactattc taatttacca atattaaatc attgtacagc tggtaaggat agaactggtg
    20461 tggggagtgc tataattctt atgattttag gggtatcgag agagaatata atgaaagatt
    20521 atctaaaaag taatgatttt gcagataaag agatagaaag atttatagaa tataagccta
    20581 aatttaagga cattccaaaa gaaaatttga aatatatatt tggtgtaaat gaagagtata
    20641 tgaaaactgc atttagaaga attgatgaag aatacattag tgtagaagca tatttatatg
    20701 gagaatttaa tttaaataaa gaagaaataa gaaaattaag aaatcaatat ctagagtaaa
    20761 aggtgagttt atgagtttaa atataacagc aagacagata aatataataa aaatattgtt
    20821 aaattcaaaa gatacaatta gtggtattgc cttaagtcaa gagataggat gttcttcaaa
    20881 aactatacaa aatgaaataa aggatattaa taaacaactt agaaatggta aaattttgtc
    20941 aataagagga gttggttata aactagaagg aagttttgat gaggtaaatc taaatagcaa
    21001 tctctatgat gatgtagata gagtagaata tataatcaga aaaatattaa ccttaagtaa
    21061 tgaagaaaaa aatactataa aacttgaaga tttagccgat tctatgtatg taagtgtttc
    21121 aacagtaaaa aatgatttaa aagaagtaaa aagaatattg gaaaagtatt caatatcagt
    21181 taaatctaag cataaacaag gtatatgtgt tttggaaagt gaagataaga tattaaattg
    21241 tatagttgac ttaagcaata agagagataa tcaattgagt ttaaatgatt ttttaaatga
    21301 tgatataaag gaaagaaaac tattaataaa aaaattatta ttagacatat taaatgaaaa
    21361 tggattagta ttaactgata tagaatttaa gagtgtgtta aataatatat tagttatgtt
    21421 aagcagacat aagtataatg aaaaagaatt tatagagaat tatatagaaa attataggag
    21481 caaaagaagc tcaattttag aaaatgaaca aaacaaagaa attataataa attcaataaa
    21541 aacattttgt aaaaatttaa aattagctac atcaatagat ataagtaatg ataaggtatt
    21601 tgaagagttt ttatataagc atattagtag tttatgtaaa aaaataaagt taggtataaa
    21661 tcaatcacct ataatggctc aagatataaa aataaaatat ccatttgctt ttgaactagc
    21721 taatatagct aaaaagacaa tagaaaaaga tttgaagata gaaattaatg aagatgaagt
    21781 tgcaaacata gccctacata taggaggagc agttgaaaga gcatcttata ataataaaga
    21841 taaggttttc aaaactataa tagtttgtac ttcaggcata ggcacatcaa tgttaataaa
    21901 agctaaatta gagaatatat ttaaggaaaa attagaaata ctaaagatta ttccttcata
    21961 cttagttgaa tatataaaag ttatagatgt tgattttgta atatctactg ttcctattga
    22021 agtaggagat gtgccagtaa taaatatatc tccaatttta agtgagaaag aaattagatt
    22081 aattgaaaaa tatatagaaa caggaaatat atacctagat ttagatataa aaagcctgtt
    22141 tgatagtgaa ttgtttttta ctgatttggt gtttaaaagg aaagaagaag ttatagattt
    22201 tatggcaagt aaacttgtac aaaagggcta tatagatgag gatatgaaaa aatcttactt
    22261 tgagagagaa aaaatagcaa caacagaaat agggaatatg gtaactatac cacatggagc
    22321 tattggaaaa atttttgata ataaaatagc aattggtata ttaaagaagc ctatttcttg
    22381 ggaaatatca gatgttcgtt taataataat gttagccctt gataaagata aaatattaga
    22441 ttatgaagta atattttcaa aaatatataa aagagtagat tctatagcaa aagttataag
    22501 tatatgtgaa aatagtagtt ttgaaaaatt tgtaaattta tttaattaga tgggtagtat
    22561 tgtatataca cctgtaaatt tggataatat aaaactaaat aagataaaaa cgataaattt
    22621 ctcttttaat taagagaaat tttttgactt tcaaggatat taatataaag ttaagatata
    22681 aacataaagc aaataaataa aaaagaggtg gcaatatgat ttatacagtg acattaaatc
    22741 catcaataga ttatatagtt aagctagatg agctaaagac aggaagtaca aatcgtgtaa
    22801 aagaagaata cgtttatcca ggaggaaaag gtataaatgt atctcgcata cttaaggaac
    22861 ttggaaatga taatacatct ttaggtttta taagtggttt tactggagaa tatataataa
    22921 gaacattgga agaaaaagaa ttgaagacag attttataaa gataaaaagt ggtttttcaa
    22981 gaataaatgt aaaaataaag gcgttaaagg aaactgagat taatggacaa ggtccaaata
    23041 tagatgatga agacattgat actttatata aaaaacttga taagctaaat caagatgata
    23101 ttttaatatt ggcaggaagt ataccatcta ctttagatga aaaactatac gaaaatataa
    23161 tggctagatt agaaaaaaag aatataaaag ttgtagtaga tgcaactaaa aatcttcttc
    23221 ttaatgtttt aaaatacaaa ccttttctaa ttaagcctaa taacgacgaa ttagaagaac
    23281 tatttggtgt taaattaaat tcaattgaag acatggtaaa atatgctaga aggttaaagg
    23341 aaatgggagc tataaatgtc ttggtatcaa tgggaaaaga tggagcattg ttgattacag
    23401 aggaagatga agtgcttata agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag
    23461 caggtgattc aatggtagca ggatttatat ctgggtattt gaatactggg aaatatgatt
    23521 atgctcttaa attgggagca gcttcaggta gtgcaactgc tttttcatat gacttagcca
    23581 aaagagaata cattgataaa ttggtaaatg aaatatctgt aaagaagttt taaaatatta
    23641 aaaaatatat agaaaggtaa ttaatattta tgaaaattat agatttatta gatgaaaagt
    23701 caattaagtt aaatttaaat tcaaaaacca aatcagaagc aatagaagaa ttagtagatt
    23761 tggtcgcaaa tagtggaaat ttaaatgata aagaaaacta caaaaaagca attttggctc
    23821 gtgaagaaat gagtacaaca ggaattggag aaggtgtagc tatacctcat gctaaaaatt
    23881 cttcagttac taaagcttgt atagcagcag cagtgtcaaa agaaggtata gattatgaat
    23941 cgtttgacgg aagtttatca aatctatttt ttatgatagc tgcaccagac ggagctaaca
    24001 atactcatct tgaagtatta tcaagattat ctacaatact tatggatgaa gactttagaa
    24061 ataaacttat aaattcaagc tctgaaaaag aatttaagga aataatagat aaaaaggaaa
    24121 gagaaaaatt cagtgaagaa tatcaagatg agaaagtggc tgaaaaaaat ataatagata
    24181 ctaaagagaa tgatgctaat aagtatccta aggttttagc agtaacagca tgtccaactg
    24241 gaatagcaca tacatttatg gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg
    24301 tatcaataaa agtagaaact aatggttcag caggtgttaa aaataagctt acaaaagatg
    24361 aaatagaaaa tgcaacatgt ataatagtgg cagcagataa aaatgttgag atggctagat
    24421 ttaatggcaa aaaagtcata aaaactaagg tggctgatgg tatacacaaa gcggaagaac
    24481 ttatagacaa ggctgtaaat ggagatgctc caatttacca tggaggagat ggaagtcaca
    24541 acgaatcaaa tgaagaaagt gaaagtggat ttagaaaagt atacaaacac ttaatgaatg
    24601 gtgtatctaa tatgttacca tttgttattg gtggagggat acttatagcg atagcctttt
    24661 tacttgatga ttatactatt aacccttcaa actttggttc aaatacacct atagcagcat
    24721 tttttaaagg aataggagat aaagcttttg gatttatgtt acctgtactt gctggatata
    24781 tagcatactc tatatctgat agaccagcgt ttgttgtagg atttgtagga ggtgctctag
    24841 caggtgatgg tggttcagga tttttaggtg cactacttgc aggttttata gcaggttatt
    24901 tagtagaagg gcttaagaaa atatttagtg tactaccagc ttcattggaa ggaataaaac
    24961 cagtattgtt atatccttta cttggaacac ttttgatggg aattataatg acatttttag
    25021 taatacctcc agttacagca attaataatg caatggttgg atttttaaat ggtttaggtg
    25081 gaacttcaaa aatattttta ggattagtat taggtggaat gatggctgtg gatatgggtg
    25141 gacctgtaaa taaagcagca tatgtatttg gagttgcatc acttgaatca gggcagtttg
    25201 aaataatggc agcagtaatg gctggtggta tggttccgcc acttgctata gctcttgcaa
    25261 ctacattttt taagaataga tttactaaag aagaaagaga ttctggtaaa gttaattatg
    25321 taatgggatt atcatttgtt actgaaggag ctataccttt tgcagctgga gacccacttc
    25381 atgttatacc agcttgtgtt ggaggttcag cagtggcagg agcacttagt atgttgttca
    25441 atgcagctct tcgtgcacct catggaggag tatttgttat accagtggtt acccatccat
    25501 ttgcatatat attagctata gctgtaggtg ctttggttgg aatgatgctt ttagcattac
    25561 ttaaaaaacc attaaatcaa gaagtatagc aattaaagga tattcaagaa cttaaaatat
    25621 gatgttttat aaaaattaat atattgctga attattttaa aataaggatt ttatgaggct
    25681 gttatataac gatttgtaat tgttatcttg acagtctttt atttttatat aagtaaaata
    25741 agtgtgtttt gctttatttg tatttaaatt gtttttaagt tagaatataa agaaaattat
    25801 atattatttt aatattcact cgaactagaa aataatattt gttgtatttt tttgtatata
    25861 aaatatattg taatattaaa aaaataatag taaactgatt gcataaaaat gatttttata
    25921 taaaatttaa gcagaagaat aaatttctaa cgaaattgga tatttcttct gtaaatacat
    25981 attcaaaaaa agaattataa gagtttagtc agcagtcgtg taagtaataa ggactttata
    26041 aatatataga aatataatga tattaatata accaagcgat ttttaattta gctttttaat
    26101 aagcttattt tttatactat ttataataaa taaactccat gatgggaaaa ctattactat
    26161 ttaatatgta aaggagtatt ttaaatgaat gaatcatatt ggtttttaaa ctcaagtcca
    26221 aaagaatata acaaattagg tgaaaatata aaaacagatt gtcttatagt aggtggtgga
    26281 ataacgggat tgacaactgc atatttatta gcaaaagaag gtaaaaaagt agtgcttgtt
    26341 gaggctgata aaataggata tggaacttct ggaaggaata ctggtaaggt aacttgtcag
    26401 catgatatat tttactctaa gatagagaaa aaatatggat tagataaagc aaaatcatat
    26461 tataatgcaa ataatgaagc tttaaattta gttgaacaaa taatagaaga aaacaatata
    26521 aaatgtgatt ttaaaaggga aacttctttt gtatttacag aaaaagagga tactataaag
    26581 aatattaaag atgaatatcg tacatgtaaa aaaataggaa taaattgtga gtatcatgaa
    26641 actatagaaa atattccatt agatataaag ggggctatta gttttactaa tcaaggtcaa
    26701 tttaacccaa agaagtatat tgatggattg gcaaaagcag cagtaaattt agggctaaaa
    26761 atatatgaaa atacaccagt agtagacctt gaaaaaggaa aaatatgtag ggttaaaaca
    26821 agagaggata atataataga agctgagaat gttataattt catctcatag tccatggtat
    26881 gatggattga atttttattt tgcaaaagaa tatgcagagc gtgcttattt aatggttgca
    26941 gttttggaaa ataaattagc agatggtatg tttataagta tagatgaccc atcaataact
    27001 tttagacaat ataatgatgg aagtgaaaat ttactgattt ttggaggagg agaccataaa
    27061 gttggtcagg gaggaactga gaaagaaata tttgatgatt tagagcatta tggtaaagaa
    27121 gtctttaagg taaaagattt taaggggaaa tggtctgctc aagacaatat gagttttgac
    27181 aatgttcctt atattggtta tatcaacaaa agagaagaca atatttatgt agctacaggg
    27241 ttctctaaat ggggaattac taatggaact gctgctggaa taataataaa agatttgata
    27301 ataaataata attctgatta taaagatact tttaatccat ccagactagg tagctacttt
    27361 tcaaaagatt ttataaagga aaatgcaaac gtagcaataa attatgtaag tggaaaatta
    27421 aaaataggta gtggagatat gcctaaaaat aatggagaag gaaagattgt taatattgat
    27481 ggaaaaagat atggtgtata taaggatgat aatggagatt tttatattgt agacactaca
    27541 tgtactcatt taggatgtga acttaatttt aacagtgaag aaaaaacatg ggactgtcca
    27601 tgtcatggtt ctagatttga ctataaggga aatatacttg aaggaccagc tcttaagcca
    27661 ttaaaacttt atggacatgg tgataatgat gttaatccta agttattata aaattatttt
    27721 taggaaggta aagtgtatat gaataaactt ttaaagccaa taactatact attgtatata
    27781 gtttatattt tatctgccgt attttttgta gcaaacaaag aatatggaag tctaggtctg
    27841 gtagcaatta gtttagttgg tactttcatt ttgagtttta taaataagca aacggaaaga
    27901 ttattgagta aagaattata tataatgctt gctttattta taatgttttc atcattgtta
    27961 ggtacttgtt ttaattttta tgatataaat tattatgatg attttttgca tttatggtca
    28021 ggtataatat cttgtattgt ggctttttct ataattaaat acttttatac acaaagagag
    28081 ataagtaaaa tgagtaaagt gtttttagtt atatttttat ttatgtttag tatgggagta
    28141 gcgagtttat gggaaatagg tgaattttta atggatactt ttgttgggac tactacacag
    28201 gccggagggt tagaagatac tatgattgat atggtagatg ctttaatagg cacaattata
    28261 actattcctt ttatagtaaa aaaatctaat atatagtttt aaatttttta tattaattat
    28321 aatggattta attaatcatg ttgagtttca aggtctatat gattaattaa atccattttt
    28381 gttatttata attattattg aatatatttt tacatataag atataaggtg atagatagaa
    28441 attgttggat tatgtaaata attaatatat aataccatta tgggcaataa atacaaatca
    28501 aatgataata tagtctattt atgtaaatgt tatattgtat actaagtata atagtaagtt
    28561 ttttgatagt ggtgtagaaa gtaaaaatct actagcctaa attgatgtat ggattagttt
    28621 aggaacagta attgcatatc ttaagtttga tgtcatgttg ggctactaga aataggtcat
    28681 ctttttcact tgaatatctt ttctttaaac atggagagta tcatatcaaa aacatatgaa
    28741 aattaagtga tttataagaa cagtaatatt ttatatgata gtatagaata gtaataaagt
    28801 attaggagga acattgagtg ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa
    28861 gtgagtcagc aaaagaagaa ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag
    28921 aaaagttgat agatgaagaa ggagttgtag ttaatattga taatgaaata ttaactaaag
    28981 tagaagtagt aaatgatgat aatgaaatag aagaaaaaat aatagaagaa gattggttaa
    29041 ttagtgaaaa taccataaaa ttagatgata aagaagcaat tattaatgat aaaaacatag
    29101 aattatgtaa aaaagaagtt caagttgaag gtgaaaaaat agatttaaat aagtttgaag
    29161 gacttgacca aaatgaaaat tataatctag aaaaaaatgt tattgaagaa aaagaagtaa
    29221 gtgaatgttt gacagaagaa gatttagagt acataaaaga aattaaaata aaaagaggaa
    29281 aaagtataaa agctataaac ttgtatacta aagaagagtg ggtttttgac actcatatac
    29341 agtgtagtaa aaaactcaaa gttccattag ggtacataag agaaaattta aaatatggat
    29401 atatggatta ctttggagat gcaataaatt atttaagtga agtattaaat atagatgaat
    29461 actgcaaaag tgaatggagc tatctcgata atagcaaatc tccatctgag atatttaata
    29521 ttctaaacaa taaaatattt agtataaggc tttctaatga aaaaagaaat gaaattttga
    29581 caaatgataa aattgaagca ttaaaaatga attatagatt tgaatgtatt gatgaagaat
    29641 atgatgaata ttttaaaaaa tataagtcta taatcaaaag aggtggaaag aaaaaagttg
    29701 aattagtaaa taaaaaaggt gacattttag aaatatttaa gtctttagaa gaatgtgcca
    29761 tttatttgca aaaggaaaag aatgaagtta tacaaatgtt aaaatatgga gatacaaaag
    29821 taggaagaaa ctttataagg tatagtttga gaagtattta atttcatttt aaattcataa
    29881 gagtttcatt tctgattcac acatttaata ttattttaat gtaaaataat aaaggtgaag
    29941 tattgtacaa ttaaaattaa ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa
    30001 tacattagat gaatctaaaa attcagataa tgtaaattct aaaaaagatg atgtgtcaga
    30061 tgaaaaaaat actgataaca aagttaaaaa aagggaaata aagctaaaca acaaaacact
    30121 taaagctatt gtagcaattg ttgcagtagg agctatatgt ttaggtggag gctatatttt
    30181 tggcaaagaa gctggaagaa aattgcctgc tacttcaaaa cactataata gcagtgaggt
    30241 agtagctact attggggata aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc
    30301 attattttat ttaaatgcaa aaactactat gactgatgac gaaatacgtt catatgaatc
    30361 aaatatgatt gaaactatga ttacatctga tatattatcc tctgaagcag caaaagagaa
    30421 aataagtata actaatgacc aactagatgc tcagtataat aatttaatga gtagtataga
    30481 atcagcaatg ggaatgacta aagaagagtt tttaaaacag tttgatttga ctgaaaaata
    30541 tataaaagat gatatgaaaa aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa
    30601 agttactgat aaagaagcag agaactatta caataaaaac aagagcaatt atttacaagt
    30661 tagagcttca catattttaa taaaaactgt tgatgataaa ggtaagcaag ttagtagtag
    30721 caaaaaagct gaacttaaga aagaagctga agaaatactt aaaaaagccc aagctgggga
    30781 agattttgct actctagcta aaaaatactc tgaagatagt agtgcagaat caggtggaga
    30841 tttaggtttc tttggtaaag gtcagatggt ggaatctttt gaaaaagcag cctttgcatt
    30901 gaagaaggga gaggtatcta acaaacttgt agaaagtgat tatggatatc atataattaa
    30961 gaaaactgat gaaaaatatc agccatttga agaggtaaaa tcagatttag taagtagttt
    31021 aacatcagaa aaacaaaacc tacttataca aaatctaaaa gaaaaatata atgttaaaat
    31081 tacagataaa tatcaaatga aagctaaaga atagataaag tcaatttatt aaaaaaaaga
    31141 aaaattaact gtatggataa aagctatttt tactc
//
LOCUS       DAMJYC010000041        30996 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJYC010000041 DAMJYC010000000
VERSION     DAMJYC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.042"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(423..1133)
                     /gene="rgaR"
                     /locus_tag="PC472_002031"
     CDS             complement(423..1133)
                     /gene="rgaR"
                     /locus_tag="PC472_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2727357.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            complement(1558..4224)
                     /locus_tag="PC472_002032"
     CDS             complement(1558..4224)
                     /locus_tag="PC472_002032"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2727358.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(4300..4562)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4863..5690)
                     /locus_tag="PC472_002033"
     CDS             complement(4863..5690)
                     /locus_tag="PC472_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2727359.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(5738..7174)
                     /locus_tag="PC472_002034"
     CDS             complement(5738..7174)
                     /locus_tag="PC472_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2727360.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(7261..8130)
                     /locus_tag="PC472_002035"
     CDS             complement(7261..8130)
                     /locus_tag="PC472_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2727361.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(8288..8941)
                     /gene="phoU"
                     /locus_tag="PC472_002036"
     CDS             complement(8288..8941)
                     /gene="phoU"
                     /locus_tag="PC472_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2727362.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(8969..9733)
                     /gene="pstB"
                     /locus_tag="PC472_002037"
     CDS             complement(8969..9733)
                     /gene="pstB"
                     /locus_tag="PC472_002037"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2727363.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(9752..10576)
                     /gene="pstA"
                     /locus_tag="PC472_002038"
     CDS             complement(9752..10576)
                     /gene="pstA"
                     /locus_tag="PC472_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2727364.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(10578..11495)
                     /gene="pstC"
                     /locus_tag="PC472_002039"
     CDS             complement(10578..11495)
                     /gene="pstC"
                     /locus_tag="PC472_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2727365.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(11876..12322)
                     /locus_tag="PC472_002040"
     CDS             complement(11876..12322)
                     /locus_tag="PC472_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727366.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            complement(12839..13519)
                     /locus_tag="PC472_002041"
     CDS             complement(12839..13519)
                     /locus_tag="PC472_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727367.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(13569..15584)
                     /locus_tag="PC472_002042"
     CDS             complement(13569..15584)
                     /locus_tag="PC472_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2727368.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILFPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(15656..16333)
                     /locus_tag="PC472_002043"
     CDS             complement(15656..16333)
                     /locus_tag="PC472_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727369.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(16913..16996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(17229..18062)
                     /locus_tag="PC472_002044"
     CDS             complement(17229..18062)
                     /locus_tag="PC472_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2727370.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNSGDSGDSSKVSVSGSTSI
                     GPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSAGLK
                     ETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREEGSG
                     TRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSLKIN
                     DVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFIKVN
                     Q"
     gene            complement(18314..20098)
                     /gene="pepF"
                     /locus_tag="PC472_002045"
     CDS             complement(18314..20098)
                     /gene="pepF"
                     /locus_tag="PC472_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2727371.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KIIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(20164..20829)
                     /locus_tag="PC472_002046"
     CDS             complement(20164..20829)
                     /locus_tag="PC472_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2727372.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(20980..21141)
                     /locus_tag="PC472_002047"
     CDS             complement(20980..21141)
                     /locus_tag="PC472_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2727373.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(21219..21728)
                     /locus_tag="PC472_002048"
     CDS             complement(21219..21728)
                     /locus_tag="PC472_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2727374.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            21934..22152
                     /locus_tag="PC472_002049"
     CDS             21934..22152
                     /locus_tag="PC472_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2727375.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            22170..24116
                     /gene="feoB"
                     /locus_tag="PC472_002050"
     CDS             22170..24116
                     /gene="feoB"
                     /locus_tag="PC472_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2727376.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(24295..25350)
                     /locus_tag="PC472_002051"
     CDS             complement(24295..25350)
                     /locus_tag="PC472_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2727377.1"
                     /translation="MTIQDYMLETAVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(25398..26198)
                     /locus_tag="PC472_002052"
     CDS             complement(25398..26198)
                     /locus_tag="PC472_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2727378.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(26191..26982)
                     /locus_tag="PC472_002053"
     CDS             complement(26191..26982)
                     /locus_tag="PC472_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2727379.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(27022..27444)
                     /locus_tag="PC472_002054"
     CDS             complement(27022..27444)
                     /locus_tag="PC472_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2727380.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(27460..27939)
                     /locus_tag="PC472_002055"
     CDS             complement(27460..27939)
                     /locus_tag="PC472_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727381.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(28071..30827)
                     /locus_tag="PC472_002056"
     CDS             complement(28071..30827)
                     /locus_tag="PC472_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2727382.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIIIKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT        10032 a         5443 c         3327 g        12194 t
ORIGIN      
        1 tatttattaa ttcattacta taggttcaat aaactataag atgtcgcttt aatatttata
       61 ttgtactcca ttaaatatta atataattaa ttaaagtatg cgtagctaaa tgaaacaaat
      121 atctatagag ttttataaat agtcattatt ttaaaaaggt aatcatattc taaaaataca
      181 tataatgcat ataaaaatca ataatagtat attgataatc aagctactaa ttaataatca
      241 tgctattata atggaaactc tacatataca tgttaaacca tattaaaaat ctataaacta
      301 ctattttgca taataaaact gtcttaatat aaataacatt ttatatatta agacagtttc
      361 aatcaatagc cctgtgatga cattaaatct tttttacgat attttcaatt acctttttaa
      421 aattaataca ttatatcaca taacaagctt gttaatgcta cttttaaatc tttaagtcta
      481 tacttactta taggtataaa ttgttcatta atagtaatca tattatccct aagatcttct
      541 attcttctta aattaacaat aaaacttgta tgacatctaa aaaagccatt ttcacacagt
      601 aatttttctg cttgtttcat gctcatatta gctttataat ctctacctat tgtatgtacg
      661 actaaatctc tctttacagt ttcaacatat aaaatagaat ctatcagtat tctattaaat
      721 ccttttttac tatttaaaac tatataacgc tccttctttc tttgaaggtt atcaatacaa
      781 agatttacac ccattgcaaa gttactatac tctataggct ttactaaata tctataagca
      841 cgaacctcat aaccttgggg ggcatactct gaaaaagccg ttgtaaatat aatttctact
      901 ttttcatcaa actttcttat ttttttagcc acctcaattc cattgatatc tttcatttgg
      961 atatctaaaa atataatatc aattttttct gggtatttgg atattaaatc ttctccacag
     1021 gaaaattcca gtatttgata atttagacat ctttcagaca aaagtttttc tgtaaactct
     1081 tttagtatgc ttctttgtat tttttcgtca tcacatataa ctaccctgaa cataatatca
     1141 cctccaaatc taaaatatca tttagtttac tttatttcaa tttccaaact gtactcacta
     1201 gctttcaaat ttaaatttct ttataaattt aaattgtcga ttaaactact tatagaaatt
     1261 ctctttaata tttaaactta aattacgata aattcctata cttttctaga tatattatgt
     1321 aaaaaaatat agtaaaataa atataatatg tatttattac cctatattaa acatcataat
     1381 atatcaaatt tttattgctt ataaatttta agacataaaa ttcatatatt cacaacataa
     1441 aataaaaata taacacatct gtaaagaatt ataacatcat atttgtatat atataaagaa
     1501 aaacatacaa aaagtataga atatatactt tttgtatgtt tttactaatt cttatattta
     1561 ttttatttta gattctaaat ttgttaatct ttcctcaaca gatttaatca tctcttcaaa
     1621 tttttctttt tttgcttttt cttcttcaat taaactttca ggagcttttg ctaagaaacc
     1681 ttgatttgca agctttccat taactctctt tatttcgcct tctaactttt tcttttcttt
     1741 agataatctc tcaatttctt tttcaaaatc aactaattca ttaagaggta taaatatttc
     1801 aacaccatca ataactactc caactgcatc ttctggaaca ttagatttat cttttgctat
     1861 ctctacattt gaagcagatg ctaaagttat aaaatagtct ttaccaagct caatagcttc
     1921 tattttttct tcacttggga ttattatcaa tttagctttc ttagaaggtg gtacattcat
     1981 ttctgctctc acatttctta tacttcttat accttccatc aataagttca tcatatcttc
     2041 ttcttcagcc atgttatctt cttcattata ttttggccat tcagatacta ttatacatcc
     2101 ttcaacagta ggaagatgag tatatatttc ctctgttata aatggcatgt atggatgtaa
     2161 taattttaat attttttcaa gaacataagt taatgtatat aatgctgcag atttagcatt
     2221 tgcatcttct ccatataatc taggtttaac catttcaata taccagtcac agtattctga
     2281 ccaagtaaaa tcgtatatct tttgaagtgc tattcctaaa tcaaacttat ccatgttatt
     2341 agttgcttct tttacaatat tatttgctct tgatattatc catttatcag ctagagttaa
     2401 gtcctcttta acactttctc tagtcatgtt tttaattata tcttcatcaa tattcataaa
     2461 tacaaatctt gatgcattcc acaacttatt tgcaaagttt ctagcaaatt ctactctttc
     2521 catatagaat ctcatatcat ttcctgggga atttccagtt gttaatgtaa atcttagggc
     2581 atcagctcca tattgctcga ttatctccaa tgggtcgata ccatttccta aagatttact
     2641 catttttcta ccttgagaat ctcttactaa tccatgaacc aaaacatggt caaatggctt
     2701 ttcattcata cagaacatac ctgcaaatgc cattctaact acccagaaga atattatatc
     2761 gtatcctgta acaagtacac ttgttggata ataataatct aaagcttcag tcttatttgg
     2821 ccatcctaga gtagagaaag gccataatgc agaactgaac caagtatcta aagcatcttc
     2881 atcttgttta aaatgagtgc ttccacattt aggacactta tctggcattt ttctagcaac
     2941 tactatttct ccacactctt gacaataata agctggtatt tgatgacccc accataattg
     3001 tcttgatata caccaatctc ttatattttc tagccattgt aaataagttt tatcaaattt
     3061 atctggaaca aattctaatt ctcctttttt aagaatatct atagccggtt ttgcaagttc
     3121 ttccatctta acaaaccatt gctctgataa tctaggctca actactgtat ggcatctata
     3181 acaagttcct acattatgat tatgctcttt tatcttaatt aggtaacctt gttcttttaa
     3241 gtctgctacg atagcttttc tacactcaaa tctatccata ccttcatatt ttccacaaac
     3301 ttcattcata gttccatctt cattcatagt atttaactgc tcaagattat gtcttaatcc
     3361 tacttcaaaa tcattagggt catgtgctgg agtcatctta actgcaccag ttccaaactc
     3421 taaatcaaca taactatctg caactattgg aagttctctt cctactaatg gaagtattgc
     3481 tgtttttcct attaaatgtt tatatctatc atcttctgga tttactgcta taccagtatc
     3541 tcctatcatt gtctcaggtc tagttgtagc tatttctaaa aagtcttcac tatcttttag
     3601 tgggtacttt atatgataaa agtttccgtc gtgttcttca tgttcaactt ctgcatctga
     3661 taaagtagtt ttgcaatctg ggcaccagtt tattattcta tttcctctgt atatgtgacc
     3721 tttttcatat aggcttacaa aaaattctat aactgcttca ttacaccctt catccatagt
     3781 aaatctttct ttatcccaat cacaagaatc acctagttgt ttaagctgat tagatatttt
     3841 tcctccaaat tcatctttcc attcccaggc tctctttaaa aattcttctc tacctatttc
     3901 atactttgtc ttgccttctt gtttttttat tctttcaaca actttaactt ctgttgctat
     3961 agaagcatgg tcagttcctg gaagccaaaa agcttcatat ccatccatac gtttccatct
     4021 gattaatata tcttgaagtg tatggtcaag agcgtgtccc atatgcaatt gccctgttat
     4081 atttggtggt ggcatcatta ttgtaaatgg ttttttatct ggatttggtt tagatttaaa
     4141 atacccttca tccatccatt ttttatatag cctagcctca aaatcttttg ggttataagt
     4201 ttttgataaa ttcgtatttt ccattgtcct gcctcctgta ttttataaat aaatttttta
     4261 aatatcttta gaaataaaaa agagctcttc atcctcttta aaaaggacga aaagctcgcg
     4321 ttaccacctt aattccaaag cttaaaatac attaacatat aattaaatta ttggttaatg
     4381 ttattttatc aactttgact ctcaaagaat ttaacggttc taaccgtcta aacttactta
     4441 tagttcagtt tagaaactcc aaagctacat tcagtaaatc ttatacttag aaaatcttac
     4501 agccagtgaa ttttcctctc ttttagtagt atttacttac tcctcttctt ctcagtcttt
     4561 aatattacat attattatat acaaatatta ttctctttgt caattatttt tattttattt
     4621 tttctgaatt ttcttaatta tgtctattct cttttatata gttgttatat tttaaaatat
     4681 gtttttacag ttattctgtc ttataatgtc aataattgca agattatttt attttaattt
     4741 taatgctttc tcattaattt tatattatat tatccttaca gtatacctct tattcgtatt
     4801 ttttatatta tatgttttat tttataaata atttacttta tcttttacta tatttaaatt
     4861 tattataagt tttcttgcca gaaattttta ggtgttatat tttctgtaat tggcaatatt
     4921 gtatatcctc tctcttttaa gattcttata atattattta aagcatttaa actttgctct
     4981 ttctcatgaa ttaatactac taattcatct ctttttcttc cataatataa tatattactt
     5041 actatctgac ttgtagaaga cttccaatcc tgtgtatcca aattccagtc ccaaattaaa
     5101 taatcatttg ccataagttt attatatgaa ccttctggca tatatggttt acttccgtat
     5161 ggtaatctta ttaattttga actctgacca gttatttctt gtagagtatt attgcatgta
     5221 ttaaattccc caactgttac ttctgggctt ttatataact tatgtatatc atgacttaca
     5281 ctatgaaaac ctattgagtt ttcttcttcc aagactctcc ttacttcttc cttgtggttt
     5341 ttcatattct gattaagcat gaaaaatgtc gctttgacat cattttcttt taaaatatca
     5401 agtagtgcat ttgtatattt tgatggacca tcatctattg ttatgtatgc tactttttca
     5461 gaagtagccc ctggaccttt tgtttcttct tgagatgctt tgtatatgat ttctcctaaa
     5521 gtattacttt gagaaagcag ttccagtgta ttttcacctt ttgaacttat attttcaact
     5581 ggaacattat taattttttc ctccaataat gttccagtta aaattaaaac agctataata
     5641 aatatcctta taaatacatg aaaaacagcc ttattatctt taattttcat cccccatatc
     5701 tcccttttat aaaagcataa gtttaagact taaaaagtta ttttttatat ttattctctt
     5761 tcttattact ttttatatct ctactaaata tttttttaac cttttcttct tctttttctc
     5821 ctaaagattc tactcttttg attcccaaag aagttaaaaa ttgtaataca tttaaattat
     5881 caaatggagt ttccatattc gtactttcac tatattcatc aacattctta gtaccttctt
     5941 ccacacttaa aatacgcttc ggacctccta cacagcctcc tacacacccc attccttcta
     6001 taaatgtagc ttctatttct ttatttagaa ctttctttag accgtccatg cactcttttg
     6061 ttccttcaaa tgccttttct tcaaatttta tatttttgtc aattcttcta actgataact
     6121 taactgcttt acttacacct cctgttctag cataaactct acctgaaagg gacgcctcta
     6181 ctctattact ctcatccatt attgctggat ttaactctaa tgcatcaaat atttcttgca
     6241 actctttaaa tgtaagcaca aaatcaactg catcttttat atcttctaac attgcctctt
     6301 tcttttttgc catacaagga cctatgaata caactttagc atctggattt aaaattttta
     6361 cagctctccc acaagcaatc ataggagaaa cggatggtga tacattttcc aatatctgag
     6421 gaaaactatt ttgtatcaaa ctaacccaaa taggacagca acaactagtt ataaagcatc
     6481 cttcttcgtc ctttttcata tgctcataat aatcatatgc ttcttttgca gtaagcatat
     6541 ctgctgccaa agcaacttct atcatatctt caaatcctat cttttttaat gcagttctta
     6601 atttaccagg gcttacatct ttcccaaact gtcctacaaa agctggtgca actattgcat
     6661 atacagggct ctttatatct tttaacaatt ttatcattgg tataaattct attttatctg
     6721 aaattgcacc caacttacat cttgatacac aatcaccaca agcactacat acatcttcct
     6781 cattacaatt aatatgctta cttcttatct tacatgactc atcttcctta ttattttcat
     6841 cacaatattt gcaagcatct gatattacag atattattgg tcttttgact tcatctaaat
     6901 ttacagcttc atgtagtgac tcttttaaat ttttatctgt atcttctgtc acatccaatc
     6961 ccatagttac cctaattaaa tttaccatag tatctttatc aaattttcca ctaaaataat
     7021 tatttaaatt atcctttaaa tcttcattta aaattttatt aggtaagttt tcaatatcct
     7081 ctatatcatc attccacact gcttttacaa gttcgccaaa aagcttcatt ttatatattt
     7141 ctagtatttt agaattgtca tttctagtat tcataaaaaa tagtacctcc ataaattaaa
     7201 cttatatatg tttaatattt acaaagatac tattttttat ttattattta actactatta
     7261 ttaatatgat ttatttagct ttctcaacaa ctctatttca atcattattt tcattagatg
     7321 atagttgaat acagggcttt tttcttcatc tatattccag tctagcttat ccacattata
     7381 tgttttctta aggtatttat atgtatgctc tttcaaataa agtttatttt caagtaactc
     7441 tatagactgc atatgaaaat gtatttctct acatatcctt aaggcattct caattttttc
     7501 tatattagtt tcactcaaac tataatccaa ctcatcaata agttttgaat atatttcttc
     7561 taatttttct acttcttctt caaatttctc tacatttaaa ttttcttttc taagaatttt
     7621 atcttcaata agcaacattg aaagttcttc tatcttttta aactcatcta taacactatt
     7681 tagataattt ggtctagcaa cataataatt aacaagtact ccaaacaaaa cacccacaga
     7741 tgtatctaac accctatgta aagaataata cacagggtca ctatttccta cacctagatg
     7801 tatagccaga aaggttacac aggcaactac tactgacttt attttaaaca gattgcataa
     7861 atatattgag cacattactc ctagaccaca taaaaatggg tctccagctt taatgattgc
     7921 aaatatgaat cctattatac cacctaaaat agtaccttcc actctattga ttcccgtctt
     7981 taaagaacct tttactgtat cttgaacaga aattatacat gctaccagag aataaaacat
     8041 attctctact aaatagtcac caagaatcat acataaagtg acactaattc ctgtttttat
     8101 agtcctcata cccatctttt gaagtttcaa agttccacct cctaaaaagc accttatata
     8161 aaatacgcat cctcatattt aatatgttcc tagtatatca caatttttta aatttaccca
     8221 ttaatcatat ttttttatca attctttctt aatttagaca ataaaaaaac tgctttaaag
     8281 cagtttttta tcctatctca accattttac cctcaattgc aaaaattatc ttctcacata
     8341 catttgttat atggtctcca attctttcaa ggtatcttcc tataagtaga agttttacac
     8401 cttggtctat ggtatttgag ttttcatgca ttatttttaa acaatcaatt tgtacttgtt
     8461 catacaatcc atctacaata tcatctttag ttccaacatc ataagctata cttgcatcaa
     8521 ataagattaa agcttctcta gtctgagaca tcatagactt acattctttt gccatcttag
     8581 gaatatctat tagctctttt atataagcct ctccacctat cttcaacatt tctctagcta
     8641 tatttgctgc ataatctcct attctttcca gctctataga tatattacct aatgaataaa
     8701 taaatcttaa gtcccttgcc attggttgct ttaatactat tagctctata ctcttatctc
     8761 ttatttcttc tcttaattta tttatttctt catcatcttc tactactttt cttgcaccct
     8821 ctaaatcctt ctctatcata caagtcacag acttttctat tgcttcttca catttttcca
     8881 tcataagcac tgtttgttct tttaaagtat caatacttat atctaaacta ttattgacca
     8941 ttgtctaact cctttatagt tatatatttt aaccaaatct acctgttata tagtcttctg
     9001 ttcttttatc tacaggagtg gtaaacatag tttttgtatc gctaaattct ataacctctc
     9061 catttaagaa aaaagctgtt tcatctgata tacgagcagc ttgttgcata ttatgtgtaa
     9121 ctatgactat tgtgtagtct ttttttaaat cctctataag ttcctctact tttaatgttg
     9181 aaattgggtc taaagcagat gtaggttcat ccataagaat aacttctggt ctcatagcaa
     9241 ttgctcttgc tatacatata cgctgttgtt gaccaccaga aagtcctagt gcagatgatt
     9301 taagcctgtc tttaacctca tcccaaatag ctgcaccttt taagctttct tctacaattt
     9361 tatctaattg cttcttatct cttagtccat gtgtacgagg accatatgcc acattatcat
     9421 atatactcat aggaaatgga tttggctttt gaaataccat tcctacttta gtacgaagat
     9481 ttataacatc atctgaggta tatatgtcct ctccatcaac agaaatgttt cctttaattg
     9541 ttacatcttc gattaagtca ttcattctat ttaacgttct tataaaagtt gattttccgc
     9601 atccagatgg acctattaaa gcagtaacct tattctcttt tatatccata tttattttct
     9661 ttaatgcttg cttatcacca taaaataaat ccaaatcttt tacactcatt tttatcttat
     9721 cgattaattc cattattcac acctcttaaa tttaataatt agctttattt aatttcttag
     9781 caacaaatct tgctagcatg tttagtgcta atacaattac tattaaaaca attcctatac
     9841 tggctgctgt tgctatatct ccactctctt tagtcagtaa gtatgagtgt acagttaatg
     9901 ttcttgcact atcaaatatt ccacctggca tcttggctac agtacctgct gtaagtaata
     9961 ttgcagctga ttctccaatt atacgaccaa ctgataagat tactccagat aaaattcctg
    10021 gtattgcact tggaagtact accttatata aagtttgaaa ttttgtagct cctaatgcca
    10081 atgatgattc tctataagct tgaggaactg tttttaaagc ttcttctgtt gtacgaatta
    10141 ttactggtaa tattattatt gcaactgtaa gtgaacctgc aactatggaa tatcccattt
    10201 ttaatgtaac tacaaagaaa attcctccaa ataatccata tactatagat ggtatccctg
    10261 ataaactttc agttgcaaat cttatagctg taactgcttt tcctttttta gcatattctt
    10321 gtaaatatat agcagccaat attcctatag gagttgctac aactatagaa accactactg
    10381 tatataatgt tgtaattatc attggaagta taccaccatc acctgatgct gaataattgc
    10441 taaataagaa attaaaattc atgttgccaa ttccctttat aaatataaat cccactataa
    10501 ctactaatga tactattgta aatagtgctg atagatatac taatgacttc aatagattct
    10561 ctttgaactt tctcatctta atttaccgcc ttatttgaaa gtttatttaa aactaagttt
    10621 aaaattaata taaatgtaaa caatactact ccagttgcaa atagcatttc ttgatgtgta
    10681 ccaaatgcgt atcccatttc aagtgctata ttagttgtaa gtggtcttac actatctgtt
    10741 attgcagttg gcataactgg tgagttgcct gcaacaagta ttactgccat tgtttcacct
    10801 aaagctcttc ctactcctaa cactactgcg gctaatactc cagattttgc agcaggtaat
    10861 acaactttga atattgtttc tatttttgat gcaccaagtg ctaatgaacc ttctttgtat
    10921 gcttttggta ctgctcttat tgctgtttct gatacagata ttattgttgg taacatcatt
    10981 actgctaata ctattactat tgctaacaaa ctttgacctt ttggaagatt aaatatactt
    11041 tgtatagttg gaactatcac tgcaagacca aatacaccat ataatactga aggtatacct
    11101 gctaaaagtt caactgctgg tgacattatt tttgctaatc ttttaggtgc tacttctgct
    11161 ataaatacag aggttaatat accaacaggt actcctatca caagtgctcc aagtgttgca
    11221 aatattgatg ctactatcat agatgatatt ccaaatttat tggcagaagg aacccaatct
    11281 gcaccagtta aaaagtctat aaatgaatat ccttcaaata taaatggtct taatccttta
    11341 taaaatacga atcctattat caaaagtaaa cttgcaactg ctactaaagc actaagtaga
    11401 aaaatatttt ttgctacttt ttcaattacg tattttgtct tattgccatt attgtcattt
    11461 ttttctaaac ctactaatct agattttact atcataatac cctcccatta aaatatactt
    11521 atcgattttc gttctgtatt caaaatttaa ttttttataa ttcgtcttca tcaaatttcc
    11581 tatactttta ttctactttt tttaagttaa atctatatta ggattaagtt aagcaagtgt
    11641 aaaattatat ttacttttta acattaattt tacttttaaa actatatttg acatttactt
    11701 tacaatatac tgttgtttct ctatattaaa atagcagata tagataataa tacagtttat
    11761 gtataattta ttacctatat ctgctattaa gatttgttaa ctaaatttaa cttttatact
    11821 acatatataa ttattgtatc ctctagttta tatcactatt atttccatca aattgttacg
    11881 atacttgatg tttgcaatag gtacgaatag ccaaaaatat aaaaataggt atttctatca
    11941 ctgagaaaaa cagtataatg tgtggtgtcc aaaaactcat acctcctaag tgtaagaaat
    12001 taaatgatgt catttgatat aataaactaa tcttaaaacc aattccccct ggtggtaata
    12061 ggtatccaat ccaactaaca cctattccca taggaattaa gcaaatagaa aaagcaatca
    12121 gcatagaagt aagagaatcc ttacattttg cagataaaaa taatgttaaa ctaaccattg
    12181 caagtattga aattagacca ccaatagcta taagaatctg tgcttgtagt aaatttatat
    12241 ttactagact aattacagaa aacaacattt gaaatgaggt tttcaaacat tcagtaccaa
    12301 aagctaaatc agaaattgcc aaatggatag aaatacatat aacaaacatt attataaata
    12361 tagaaaatag agctaatatt ttagtaaacg ctagacgaat atgtccatgt ttcgtacaac
    12421 gaaggatgct atctgattgt gtctgatact caccagaaaa agtaggtgca gcaattgcaa
    12481 cacatataat taataatata aaaatataaa atattatata atcgaaagca tcttctggat
    12541 ttattaagaa attcttaatc ttattattta actattttat tatttaatat atattaaagg
    12601 taaaaaataa tatatagatt tagatataca atttatttat cttttcaaat ataatctata
    12661 aaataatagt tctgttacgc atttttttat ataataaaac tgactgtaat ttaattggca
    12721 aagtctagaa gaataggctt tatcaattaa attacagtca gctattttat aataatttag
    12781 tttagatatc gttagtattt cacatttata acaattatta atgatattaa tatatttatt
    12841 attctttatt aaaataatat cctgctcctc ttattgttat aattttctga ggatttttat
    12901 aatctttctc tattttcttt cttaatccat tcactgcaac taccacacta tagttatcct
    12961 ctaagtattc attcatccat acatgttcat atatctcttt agtagtcaaa gtcttttcag
    13021 gattctctat aaaaaagttt aacaacttat tttctgttgg agtcaatttt attattttat
    13081 cttctaactt tattactcca ctagttctat tatattcctc taattttgta ttattttcat
    13141 tattatttac atatgagttc attcttctta aaatagcttt tacttttgcg tataatattt
    13201 ctaaatttag aggctttatt aagtaatcgt ctccaccaga atttaaagca tttaaaatgc
    13261 tttggtcttc atttatataa gtcatataaa caattggaca agtagttaca tttctaattt
    13321 ttttacaaag agtccatcca tcaccatcac ttagaattat ttctaaaaat attaaatcat
    13381 atttattgct aaatattttt ccaatagctt cttcacaatt atatgctaaa tctatactta
    13441 ttcctttttc ctccagaaat ccttttagct tctggcaatt atccttgtct ccatctatta
    13501 tcaatatctt agtttgcatt ataatcactc ccaaatatca ttattctttt atattaaata
    13561 agctattatt ataaaaaaca tagacttaat tttattgtaa aaacagttcc ttttcctagt
    13621 tctgaatcta caaagatttc tccaccatgc tctcttataa tggatttgac aatatatagc
    13681 cctaaccctt gcccttttgg ctcattattg tctctttctg taaagaatct gtcaaatacc
    13741 tttggtatat tttctggagg tatacctttt ccattatcag aaattttaat aattgcaaat
    13801 ccatcttctt tatccattga taaaattatc tttccatcaa atggagtaaa ggtcaaagca
    13861 ttataaatta aattctcaaa tgctcttgta agcatctctt tatttatctt tgcatatatt
    13921 tttttctcca atacgtaagc ttcaaagttt acaccacatg cttgtgtatc tggcaaatta
    13981 ttttcatata tctctttaat aaaattattc agacatattt tttcactaat accaatgctt
    14041 ttatcttgtg atgtaaattc ttgcaaacta cgtactctat tttgtacttc atttgacttt
    14101 tgatttatag cataaagata ggttcttgtc tcttcatcta catgaatatt accaaccttt
    14161 atcaaatcta taaatgcttg tatagccgaa acaggtgcct ttaaatcatg tgctacatct
    14221 gccaagaatc ttttacgttc atttaacaat gttgtaagtt cattagttct cacttcaact
    14281 tcatgttcaa gattccttgt aagtttttca ttctctttta ttattattct attcctgttt
    14341 atcatcataa cagaaaataa aataacaatt acaaacccac aatattctgt ttgccaccct
    14401 gtaaatatag gctcaaatct attactcgta actgcatcaa ataatatgct aattccaaaa
    14461 acagtgttta ctgaaactaa aatataagaa cttatatgct cagagcatgt ctttttatat
    14521 attgaaacca gcgaagacat taatatatat ataaatacaa tatatttata tatgtctatt
    14581 aacttcccat atatgttgac aaagtacata tcatatggaa aaagaataat tggtatcact
    14641 atagatatga tactcattgt aattcctaat ggtaaaatta aaaatctgta cactttattt
    14701 tcttctattt tggatatact tccatttata caaataatac ataaaactac cataaaatat
    14761 gacaaatctt caaccgcata tgtaaagctt accaaaggca gtcctaacaa atgtacaaag
    14821 ggataggata catggatggc aaagaaaaca cacatacatc caaaatataa gtaaatttta
    14881 cttttgtttg aaagaaacca tacagacaat gaaaatatag ctattgcaaa agttgtgaag
    14941 caaattatac tataaaaaag taatcgataa aacatcatat tacttatgtc ctttgtttct
    15001 cctaatgcag gcggataata caaaccacta taataatgtg aaaaattcga tacattgatt
    15061 ataatttctg tatttttctt tgcaataaaa cttactactg aatttttaat tttgggtcta
    15121 tatttttctg ttcctacatc tcctaattta gaaaccattt ctccatttat ccatacttca
    15181 cttgcggaaa aaatctcagg aagctctaaa cttagtagct tatccttgcc ttcatttttt
    15241 attctcatac gataagtagc attcccatat ggtgactgag tcccctttct catagaaaaa
    15301 tctggatatt gacctataaa aacatattct ggttttatat cttgctctgt aaaatcattc
    15361 ggtttaaata ttttatgatt ataaaactcc cagtcatcta tcaaaaagac aagtttacct
    15421 ttagtgaaag tatcattatc tatagttaaa attccattac ttgctctaat agacttatat
    15481 gtatatttat tatcatattt atacagaaga tttaatcctg ttattgccaa cagagagact
    15541 atcaatacaa tgagagttcc ttttagtata ttatactttt tcattgtatc aaccacatct
    15601 ctttcatagt agtattaaat aaaacaagaa ctatttatta attacagtat ctcacttatg
    15661 gtacaaactt atatcctttt ccccatacgg tttgaatgta ttgatgctcc ctgcttaaag
    15721 aattaatttt ttttctcatc tgtgcaatat gaacctgcac tgtatgttga tctccaatat
    15781 caggcatttt ccaaacttgg ttatatatct ctatcttcga aaatacttgt tctttgtttt
    15841 cagctagaaa caataatatt tcaaattcca tagtagttaa atctatactt tctccatcaa
    15901 aagttacttt tcttgaattt acatttatta ctaacgctga aaactttaac acattaggaa
    15961 ctgatgtaat atttttgtac tgcctcatac gagcatatat tctaagttca agttctttta
    16021 aactataggg ttttgttata taatcatccc ccccagctaa aaatccccga actttttctt
    16081 cttcatgctc ataattagat aaaaatatta taggtatgtt aaccttttct tttatatcat
    16141 tgcacatttt atatccatct atatctggca atgaaatatc caaaattata cagtctaaaa
    16201 ctatactctc tactatatcc atagcatctt tagcagtctc tgatgtatat acattaaaac
    16261 ctatattaga aaaataaatt tgatttatgt ttaaaatatc aacatcatca tctacaacca
    16321 gtaaattata cattacacca ctccattcaa aggtatttta attataattc caagttttat
    16381 agttattaca aattataatg ttttattttc atatataact ggtattatta ttttataata
    16441 tctttacaaa ctagactata atacttaagt atttttgaat aattcttgaa tattgtatta
    16501 aacattaaga ttcattatta aatactaatt aaagtctccg attgttggag aatggagctt
    16561 aaagtcattt tcacgtataa atttattatt tttctagaaa cttgtctatt ggctagtttg
    16621 agtattgatt tatatgattt atatggttat aatataaaat aaattgtatt attttggtaa
    16681 atatattgtt acaaaaggtt tatattctgc atctcaatgt ttataaaaac tttattttct
    16741 tattataata ctattattct tttaaaaaaa tttccctttt ccaacaaatg gctactttaa
    16801 aagccaaaat tttacctatc aataaaaatc ttaacgacta atactcctat tttcaaattt
    16861 tgcaacatat ttgttaattt taaaacaaaa aaagactatg caatataaaa attatattca
    16921 tagccggttg caccactaac tccatatatc tctagatgcc caataataag atatacttct
    16981 cagtttctaa aatattttta ttaaaaatct tttatatatt ttcataaatt tcttttttat
    17041 tctaacattg taatattatt ttgtcaatat ttccatattc ttagagataa gatactaaaa
    17101 tacctatttt atctaaagat atttatattt ctacattcaa taatagacaa taccctgcaa
    17161 gttaaagcat catttttatg agcttatctc acagggtatt atatgtttac tttatattat
    17221 aactcttgtt attgattaac tttaataaat cctttttctg aaacaagtgc ttgtccttca
    17281 tcacttaata tataatctat taaagcttta cctttttctg acaaaccatc ttctttatta
    17341 actaatataa atggtcttga tattggatat ttattagcta ttacatttgc atcagttgct
    17401 tcaacatcat ttatttttaa actatttact ttctcatcaa catatccaaa tgatatatat
    17461 cctatagcat tttcatttcc ttcaactgta gtttttatat ttcctgaacc atcacttatt
    17521 tgtgcatctt tagttagttc ttctgaatca aaccctacaa tttcttggaa tccatctcta
    17581 gttcctgaac cttcttctct tgatactatt actattggtg catcatttcc tcctacttct
    17641 ttccagttag taatctttcc tgtatatata cctttaactt ggtccattgt cagatttttt
    17701 actgagtttg cagtattagt tatcaatgct ataccatcta ttgctatttt agtttccttt
    17761 aatccagcac ttttttcttc atcttttaaa tctcttgaac tcattcctat ttcagatgtt
    17821 ccttctatag cattttttat acctccagaa gaaccaatct gttgtacttc tacactaaca
    17881 ccttgatttt tttcttgaaa cttttctccg atagtttcca taagaggacc tattgaagtt
    17941 gaacctgaaa cagaaacttt gcttgaatct cctgaatctc ctgagttaga gccacatcct
    18001 actgctaaag ctccaactaa cattgtacta gctaaaagaa cgcctatttt ctttttaaac
    18061 attttactta cccccgctta tattttaatt ttttgttgtc atttttctat attttaataa
    18121 tacagattct ttgttaaatg aatattagag cagggttaag taaatgtaaa tattgttaaa
    18181 tacatgtaaa aacttaattt tatatgattt attcttaatt agattaactt tttaaagtta
    18241 ctttaggcag atataaacat aatacaactc atattatcag aagtcttcat tgaagctatc
    18301 aacctatcac atcctataat tctttttcta gtttattaac taattcagca aatacattta
    18361 aggcttcctc tatagattct tttttcgtca tatctacacc agcagatttt aattgttcta
    18421 gtgggtattc tgaccctcca ctttttaaga agtttatata tctatctact gcagtacttc
    18481 cttctgaaag tatctgttga cttaaagcac ttgcagcaga aaaccctgtg gcatacttat
    18541 atacatagaa gtttgaataa aaatgaggta ttctcgccca ttctattcct atttgtttat
    18601 ctactattgc agattttcca taatactttt catttaattt gtagtatata ttagtaaatt
    18661 catcagcagt aagtggttct ccactctcta ccctttgatg tacaattttt tcaaattcag
    18721 caaacatagt ttgtctatga actgtagttc taaattgttc taaataataa tttaataagt
    18781 atatagtttc atccttagac ttagaatttt ctaataaata atttataaga agtaattcat
    18841 ttaatgtaga tgcaacttct gcaacgaata tcttatagtc cgaatataaa aatggttgac
    18901 ttgttcttga ataatgactg tgaacagagt gccctaattc atgaattaaa gtgaatagag
    18961 aattaaggtc atttttatag ctcattaata tataaggatg tgaatcataa cttccccaag
    19021 aataagctcc tcctttttta ccttcattat catatacatc aatccaccct tcatcaaaag
    19081 cactttttat gatcttcaag tattcttctc ctaaaggttt aagtgcctct aatatgatat
    19141 cctgtgcttt atcatatggt atatccatgt caaatttatc tgtcaaagga acatacaaat
    19201 catacatgtg tatttcattc aatcctaaaa atttcttttt aagctcaaca tatttattta
    19261 gattaggaat acttttatct atagctgata taagattatt gtaaacatct aaacttacat
    19321 catctgaaaa taaagacgct tctattgctg aattatactt tctagttttt gcgtaaaata
    19381 tctctgattt tattccacca taaagcgttg aggcaaatgt atttttatat ttctcatatg
    19441 ttgaaaattc accttcaaaa gcgtcttttc taactctact attcttactt tttataaata
    19501 aagagtagtt tgaatgagaa agcttaacct tattcccctc ctcatcttct atctcaggaa
    19561 attccatatc tgcatatgct aacatatcgt atacattttc tggaattgtc gataattctg
    19621 atgtagctgc aagtatttct tcctctcgtt cactcaaagt atgtggttta tctctcaata
    19681 tatcctttat atattttgca taaaaggata gcttctcatc ttttaagtat tcacttaact
    19741 tattctcttc tatcgccagt atttctggaa ctatataaga agtagccaca cctaaatcag
    19801 tagataacat ttctgtctta gtggctcttc cttgattatc attaattcta gtatcttcat
    19861 gttgtttcat atgagtatat acatataaat tttgaacaat cctagatgca ttttcagaga
    19921 tatttaatac tttatataag ttctccttac tctcagaaag cttcccttta tattctttta
    19981 cttcttggat aagatttttt actttactta tgtctttttc aatttcctct ttactggagt
    20041 acatcttatc tatcttccat ttgtactttt cttctattgt acttctatca gttcccatat
    20101 catacacctc tattttaatt tgttttatat tttattcact ttacaaaata tattgtatat
    20161 attttagtcc aaataatcta catttattac tccttctatc atatttattt ctttcataac
    20221 gtcgtgtaag tgtattctct tagcataaga tacaaaaaaa ttaaccttcc agtattcttc
    20281 attacgacat tctatttcca tatttcttac gataagttga tttttttcaa atttatcata
    20341 taaaaaaggt atagagtcca aattgcttaa ttttaatatc aacacattct tttcttggct
    20401 aaataaaaac gtattgatta tcctaagcac aaataatgct atcattacaa ataatccacc
    20461 tagaattgcc aattcataat aaccatatcc aactgctata ccaatacatg cagttgccca
    20521 catcccagca gctgtagtaa gcccttttac tccaccttta ttttttaaaa ttgctcctgc
    20581 acctaaaaaa ccaattccag ataatacttg agcaggcatt cttgctgggt cagcatttgt
    20641 atacaaatta taatcaaaga ataattgaat cgaagttatt gaaacaatac aagaacctat
    20701 tgcaattagt atatgtgtcc taaatcctgc aaactgtctt attttttctc tctctaagcc
    20761 tattattcct cctattatta gtgctaatag taaccttatt gctatctcct tacttcctat
    20821 atcaatcata atataaaacc ctcctgactt aattacaact tatgtcctac agataattta
    20881 cacatcagaa atactatttt atatcttaat attattatta atatatgtaa aatatagcca
    20941 tctgttaatt tttgatacaa aaaaacccac aactgtgggc tatttgctta ggtacatgta
    21001 tttatttatt aatatatcta ttttcttact tgattctact aactcttctt gaaatatttc
    21061 tggatattct atgtatgtat ttatctcttc cctaactgct tcaatctcat tttttaacgc
    21121 ttctaattca ttgttcgcca taataatcac cactattaca aatttgtata tttttatata
    21181 cattttatca aacaatatta tattttgtca atattatgtc aataaaactt actataaaaa
    21241 tgctttaatt taggtattgt atacctggat actaagccta caaacatacc agttactaat
    21301 gatgccatta acataaatgg taagtaccct atagcaagta tattttctat gattaaactt
    21361 gcaactatta attgacctat attatgccct attgcaccta taatgcttat ccctattagt
    21421 gataaatctt ttattttact cgctaaatac ataaataaaa gactcagata agctcctcct
    21481 atactaaata gtagatatga taaagggcct gcaaatattg atgacaaaat taccctgata
    21541 gtaacaatta taacagtatc tttgacacct aacaacatta atgaaattaa agttataata
    21601 ttgctcaaac ccaattttgc ccctggaaat ataactataa gaggatttgg tatatatgtc
    21661 tctaatacat ataaagctaa actataccct atcataagac ctaaaaatgt catttttcta
    21721 gttttcatta agttaactcc tccaaatttt atctatcatc ttacattata atacaaaata
    21781 tataaaaaca cctaatttag actaaaatat ttttaatcta tttagaataa atattagtta
    21841 tttcaatgtg caattacctc tataaatctt gactttttgt atcatacttg ttacaatttt
    21901 aatgataatg aatttcatta ggaggtgtta ataatgactg tttacgattt aaaaatagga
    21961 gaaaaaggat ttattaatag tatagatgga gatattaaat tatctaaaag attgcttgca
    22021 cttggatgta taaatagtac tgaaatagaa gttaaaaagg ttgccccact tggagaccca
    22081 attataatta gatttagagg ctttgacctt gcgattagaa aatctgatgc taaaaatatt
    22141 tctttacaat aatagaggag gagctacccg tgattaatgt agcattatta ggaaacccaa
    22201 atgtcggtaa aactactgtt tttaatctac ttacaggttc aaatcaatat gtaggtaact
    22261 ggcctggtgt tactatagaa aaaaaagaag gatttcttgg aaaagagata aaggttgtcg
    22321 atttacctgg tatatatgca atggatactt tctcaaatga agaaaaggtt tctaaatcat
    22381 atttagaaaa tgaagatgtt gatgtaatag taaatgtagt tgatgcttct aatttatcaa
    22441 gaaaccttta cttaacaact caacttatgc aatttaacaa gcctatagtt atattgctta
    22501 atatgttaga cattgcagaa tctaaaggag ttaatataga tgccaaaaaa ttaagtgaag
    22561 aacttggagt aattgttgtt ccaattatag caaaaaagaa aaatggtgtt gataggatag
    22621 aagaagagat taagggagct acgcaaaaag cttttatata tcagaaaaac tttggaagtg
    22681 aaacagaaac atataaaaaa ttagaatcca ttttatcaag atgtacaaaa actacatata
    22741 cacaaaagaa atctatcagt gataagatag acaatatagt cttgaatcct atattagcat
    22801 atccaatatt tattggtgta ttgttcctct tatttaaatt tacctttgac tgggtcggag
    22861 gtccactaca agagggattt gctagtctta tagaggctta tatttctgca cctgttagtg
    22921 atatgttatc taactctagc ccatggttta aatcacttat agttgatgga ataattggag
    22981 gtgttggtgg tactttacca ttcttccctt taatatttac attattcttt ggtatatcgt
    23041 tgtttgaaga tagtggatat atgtcaagaa cagcattttt aatggataaa gttatgagaa
    23101 aggttggtct atctggtaaa gcttttatac ctatagttat gggtatggga tgttcttctc
    23161 cagctataat ggcaactaga acattggaaa gcgaaaaaga tagaagagtt acagctctta
    23221 ttgcaccact tatgacttgt ggagcaaagc tacctatata tgcactattt gtagctatat
    23281 tctttccaca taatgctgct ttagtaacta catcactgta tttggtgggt atagttatgg
    23341 caattgtagt tgcattattc ttaaataaaa cagcctttaa aacagaggct gaaccattta
    23401 tattagaatt accagaatat agaatcccta ctatagatgc acttattaaa aatacttgga
    23461 ataaatcaaa aggtttcttg ataagagttg ttactgtcat gtttgcaatg tcagtcgtaa
    23521 tttggggact ttcttcattc aatgtttttg gatatacaga agatataaat gtaagcttct
    23581 tggctactct aggtcatatt atatctccta tattcaaacc tcttggtttt gatgattgga
    23641 gaacttcagt tgctatttta agtggtcttg gagcaaaaga aatcgtagta aactctttaa
    23701 atatactata tggtaatcta actgttgtct taccaacaat atttaatgga gttactgcat
    23761 atacattctt gatatttact gctttatata ctccatgtat agctgcactt gcaactatga
    23821 aaaaagaata tggcaataaa atgatgttta catcatttgc atatcaattt atactagcat
    23881 ggataatggc ttttatagtt aaaagtatag gtggagtact atttatgggt aattcaataa
    23941 tagaagcttt gataggtgga ataattatag tccttgcact ctttattctg tttaataaat
    24001 tcaagtctac aaaaaacgga aatgtctgta gtggatgttc tatttgttct ggatgtccaa
    24061 gtgctaattc ttgctcaagt aaggaagaca gcaataaaaa aaataatgca cactaatata
    24121 taaatatttt agtttaaaat aagataaaag tgggtgtctc aaaattaact ttttagttca
    24181 tgaggcactc ttttttatag aaaatcaaat atattttcat catttcaaca gtaaaaaaga
    24241 gcttatctag taaaaaagag gcttatctct attttgagac aacctctttt tttgttactt
    24301 attcagtttc ttgctaccta acttcatgtg gaacctagta tcttttggaa cacttgggtc
    24361 tacacctctt aatgtaggta tttccccaca tattagttgt ataggtacta taaaattaaa
    24421 tgtttgatag taaggattat ctgtgatatc aatctttaaa tttttatcat tttctgtaac
    24481 atctctacct atagcaaata cattttccgt ccaaccagaa agtacatcaa tcatcttagt
    24541 tactctaggt tcttttcctg tatctagtat aaatatagta gaatcactat ttatagcatt
    24601 atatatacca tgtataaact cttcaaactc atacccagtt acaggtattc tcattgtttc
    24661 caataatttt aatgctgctt ctaatgtatc tccatatata tcagagtgac ctattattct
    24721 tatttcttta gaattaacta acttttcttt gtttctttca atccattgtt tagagagctt
    24781 atacactgct tcaaatctat taattgcgtc taatatctta tttatttcct cattgtactt
    24841 ttcactagat attatacctt tttctctagc tatttgaaga cctaataaca ttaagtttaa
    24901 ctttgtacaa taatatcctt ttgttttagc tccagatttt tcttcaccac aatttactgt
    24961 taatatataa tcagaaatct catctattaa agcattctta caacctgcca tagatgctat
    25021 tttacagccc ttatcttcag ctaacttcat agcgttgtat gtagaataac tactcccacc
    25081 ttgagataca cctactacta gggtattttc attatcaaac ttgaatgtat cttcagttat
    25141 cataaatgga tacattttca caacatctat atctaataaa ttttgtaaat atggttgtac
    25201 ttgcactcct gagtgatagc tagtacctga acctgttatt ataatctttt ttagatttgt
    25261 tctcttaact tcattaaata agctatctgc attagatata atttctctca ttctaactgc
    25321 tgtttctaac atataatctt gtattgtcat tttatacacc ttccttatct atatattcaa
    25381 taaattataa ttaatttcta taacaaacct atagcatgta aagcgattcc tgttactata
    25441 gtgcctatag ttaattttgt tacacttacc tttctcttta acatgtagta tattacaaat
    25501 gcaaaggtta aaggtaacat attaggtatt attttatcta gtatatcttg taaaattact
    25561 tctgcaccat ttaagttgaa tttcagagga gtagttatac tcaacatagt tgcaaccatt
    25621 cctcctacaa cgaataaacc aacagttgta gccatagaca ttattttctc cataagtccc
    25681 tctctttgta ttctttcaag agaatttacc cctacttgat aaccatactt aagtccaaat
    25741 attctaaggg caaatgctgg tatattgtat aatagtaaga ataatagtgg tccaaagata
    25801 ttaccttctt ttgcaagtga tactcctact cctgctgcta taatcctaaa tgttccccag
    25861 aataatgaat caccaattcc tgcaagaggt cccataagag ctgttttaac agcactaatt
    25921 gaactttcat caaattctgg attattagca ttctcttctt ccatagctgc tgttatacct
    25981 aatatagcag gaactactac tgtcgttgta ttaaaaatct ccaaatgcct ttctattgct
    26041 tttgcttgat tttctttctt attgtatagc ttctttattg caggtaacat tgaataacag
    26101 taacctaagt tttgcattct ttcgtaatta aatgcacctt gaagagcaaa tgaacgccaa
    26161 aacatacttc ttaattcttt ttttgttaat ttattcaaag tcaatctctc cttccgttaa
    26221 agtattgatt gctaattgag gattttgatt attttctttg ttattgatat aaaagtttag
    26281 tacaaatgct acacatactc caaataaagc tatagcagtt atatctaatc caccataaga
    26341 tgctaacaaa aaccctataa agaaaaatac agacatcttt tttgaaagta acatatccat
    26401 aagcaatgca aaacctactg ctggcaacaa tgttcctgca acacccagtc cgtctaatat
    26461 aactttaggt attgcattta tcaatgctgt aactttatca gcaccaatca taattgctaa
    26521 aaaggttgga actgatgtac ttaaaaagaa taatattact ggcaaccaca tcattatggt
    26581 aactttctta aaatcagctt gtttagcata gaaaactgcc ttttgagaaa agtacgaatt
    26641 tattattcta actaatactc caagagattg tgctaagact gctactggta ctgctactgc
    26701 taaagcaact tctgcaccat tacctgataa tatagcaaaa gctgtaccta atacacctcc
    26761 tgttactaca tctggaggag tagctgcacc tattcctgca attcccatcc aaattaattc
    26821 aagctgtgca ccaatcataa ttcctgattt tatgtctcct aaaactaatc ctactaacat
    26881 acctgtaact attggcctat caagcataga ttgaccaaat atacgcccat ctagtatacc
    26941 aactcctgct ataatagcta acatagtagc ttgtactaac atattgaacc tccattttaa
    27001 cttagtttaa tttacattaa attacattac atttttgatt ttgttttgat tttctaataa
    27061 ctcatttaca taaacaattc cattttttgc ttcctctaat cctgatttaa ctattagttt
    27121 tgtatcctcc tcaactaata aaagtataga taaaagcatt ggtaaattta ctcctgtcaa
    27181 taattcaaat ttgaaccctt gtaaaagtaa gtttgtacat atgtttgcca cacttccacc
    27241 aaaaacatct gttaagacaa caaattcttc actatcttta ttatttgata atatacttct
    27301 aatttctttc tcagcatcat ctgctgattt atccgttgtc atacaaaaat actcaatatt
    27361 agttaatgaa ccaattatta atttacttgt gtcaacaagc tctctggaca attctccatg
    27421 tgttgcaatt agaaattttt tcattctctt tctcctaaat tattttatta aatctgatag
    27481 ttgtttcttt ttgtctctag gtaacattct aaattctata tcaatcccta aatcacctat
    27541 ttgtttaaag ttatttatat catcatcatc aacagcaact aaatctgtaa gtttcttctt
    27601 cccttcctca tatcttaatc ctcctacatt tatttctttt aatactcctc ctgaattttt
    27661 agctacaact aaagcatctt tggtattttt tactaacact aatacattgc ttttctttga
    27721 agaagcaaat ttttctacta tttctccaga ttcctctacc ccccttatat ataaagttac
    27781 attgctaggt ttcgctaagt tcagtgacat agctttcatt tggtcatttt ttgcttcatc
    27841 atttgcaact agtattacat ttatacctag tgatgttgcc catccatatg caacctgacc
    27901 atgtatcaat ctgtcatcaa ttctcatcaa agataccatt ttccccaaat cctccatcat
    27961 acattaaaat ttttattgtt tatactctca tttatctata aaagcaaaaa gtatgccaaa
    28021 tactggcata cttttttaaa tttcattcac ttattttgta atcttgtatt ttactgtggt
    28081 agaccataga taatattata aatatatcct atctctgaaa ttggtatttg cacactataa
    28141 ctcttttcta gcacactaaa tgcatttttt attagtttta cttcactatg atggtttttg
    28201 attaacaaat ccatatcagt atactcttca atttcagttt gtctaactaa tctttcaatc
    28261 atacaactta agtgaacata taaagaaatc tttttatcat ttgaaagttt tttcttttga
    28321 atattttcta tagcattaat tccttcttct attctctcta ttatcttttt tgaatctaaa
    28381 atagttagac tatctattac tctaattaat gaaaaattct ttacaataga atcatttatg
    28441 ttctttattt tttcattttc tactactctg ctaaatattc taaaaacatc atcttctcct
    28501 tttcctgata ttatatcctc taataatata aaatctaccc cattcacatt tggattggca
    28561 gttcctacta tagccatcac atcatacatt tcaaaaactg gttctttatc tcccatagaa
    28621 acaagactat cataatcata aggtattatt ttgacatcaa ctattccaat taaactttcg
    28681 gaaagtagat gttggatttt tatagcagta cctatccctg taaaacaaga tgatattata
    28741 gcatattctt tatttttttg aggataatac aattgtatat taggtttagt ttcttcatcc
    28801 ataatttctg caatctcttc aatgttttta ccttgcatta ataaatttcc tacttctagt
    28861 gcagaaatag tggatatttg gtctataaat aacattgggc tatttatctt attctttaaa
    28921 tcttcatata taagattaag agagcccata tcaaataaca caattaaacc attctttatc
    28981 ctatctccct ctaggtatct agacatttgt tttgatacat cagcaatagt agcatctata
    29041 ggcatatcaa tagctgtaaa tatattttta cccaacatcc gattacatac attcgccata
    29101 ctactggcag tagcatatcc atgtgctata atagtcgcat taacttgatt aaatttcatt
    29161 cttataattg ttgatttaat aaatataatc attatgatta tatctacatt attaagatac
    29221 atatccaatc tattcgttat taatgttgat attttaatta ttatagagta ttctttgtaa
    29281 ctctcttttt ttaaatatat tagtaattta ttttctaaag ctttatactc atcagacaat
    29341 tgataagatt cgtccttcaa ataaagataa gacgaaaatg ccaatacatt attcccatca
    29401 tattttatat tgttattaac ttctaaattt ctaaatatat cctgcattat cgatattata
    29461 agctccataa gtacgttatt attttcatca ttatttgaaa agactaaatt gtccattagt
    29521 ctaatcatta gattagaact ctttttacta aatttttcct ttgttatttc atttttttct
    29581 agtctatcaa agtaatctaa acattttatg aaactttgat agattatatt tcttttattt
    29641 cttccatttg acatttcaat actaaattgt ttaggagaaa agataatgct tttgctttta
    29701 ccactttgga attgaaatat atctacattt tctatataaa gatttctagg aaggtcttga
    29761 agagtaacaa aaatatttcc atttcttctt tgattttttg cataagctcg tccacatgca
    29821 tattttatta catttttaca ttcacctata ttccccttga attttatctt taaaatcgca
    29881 tttaatgtaa tttgagttat ctctataggc aaaccaagta ccttactttc ttcataaata
    29941 aaatgttcta caatctttct tttttcaata gtccctcttt cttctatatt gggaacataa
    30001 atatttatgg gtattcttct taaaaaggtt tgtaaaaatt cagatagact ttctgttgta
    30061 gcaaatatta atcttaccct agcttttcta attactccat tctctccaat cctagaaaac
    30121 gtacctgtat ccataaatgt aaataacttt tcctgacctt cactatttaa tctatgtact
    30181 tcatctaaaa ataaaacacc accatctgat tgttctatta gacctgctct agattcattt
    30241 gctccagtat atgctccttt tacatgccca aataatagac tagacaataa ctccaaatta
    30301 ttataatact gcgcacagtt taacacattg tatggagaat ttttttctaa tacattactg
    30361 cttacactat atttgtatat tatcttggct aaaaaagttt ttcctactcc actttcacct
    30421 gtcagcatta ttgtaagtcc gccatttgga taaagtacag cggtttttat ttgttcgatt
    30481 acttctttta agctattatc atatcctatg atactattaa acacagtctc ttgctttaat
    30541 aatttacact catttttaag ttcatctaaa ttttcataaa tacttttttt tgtaggtcct
    30601 actacatcat ttattttatt tttaggtaaa aaaataacag gtctactgtt tatctttata
    30661 agattatcag attttactaa atcatttaat aatctacttg attgagttct acttaaatta
    30721 aactttttta ctatatcttc tgttattatg ccactttctt ttttaagttc atcaaagttg
    30781 ttaaataata cacattcatt tacaaaactt aaaatttcat ccctcatgtt caacctccaa
    30841 tacaaatatc aataatacta taatattagt atatcattta tatatttatg aaaaagataa
    30901 ttatattcct atcttgattc tcttttgtaa gattgtaaaa tcaaactttt tataagtata
    30961 aaatctagat tttttaacat aatctcaagg ttctta
//
LOCUS       DAMJYC010000042        30930 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJYC010000042 DAMJYC010000000
VERSION     DAMJYC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.043"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(146..682)
                     /locus_tag="PC472_002057"
     CDS             complement(146..682)
                     /locus_tag="PC472_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2727383.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFDSDIEEEN"
     gene            complement(700..2013)
                     /locus_tag="PC472_002058"
     CDS             complement(700..2013)
                     /locus_tag="PC472_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2727384.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2368..2808
                     /locus_tag="PC472_002059"
     CDS             2368..2808
                     /locus_tag="PC472_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2727385.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2993..3181)
                     /locus_tag="PC472_002060"
     CDS             complement(2993..3181)
                     /locus_tag="PC472_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2727386.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4214..4609
                     /locus_tag="PC472_002061"
     CDS             4214..4609
                     /locus_tag="PC472_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727387.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4652..6751
                     /locus_tag="PC472_002062"
     CDS             4652..6751
                     /locus_tag="PC472_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2727388.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6976..12186)
                     /locus_tag="PC472_002063"
     CDS             complement(6976..12186)
                     /locus_tag="PC472_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2727389.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12201..14696)
                     /locus_tag="PC472_002064"
     CDS             complement(12201..14696)
                     /locus_tag="PC472_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2727390.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15333..15620)
                     /locus_tag="PC472_002065"
     CDS             complement(15333..15620)
                     /locus_tag="PC472_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727391.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15640..15915)
                     /locus_tag="PC472_002066"
     CDS             complement(15640..15915)
                     /locus_tag="PC472_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727392.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16550..16729)
                     /locus_tag="PC472_002067"
     CDS             complement(16550..16729)
                     /locus_tag="PC472_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727393.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17014..18480)
                     /locus_tag="PC472_002068"
     CDS             complement(17014..18480)
                     /locus_tag="PC472_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727394.1"
                     /translation="MKRKPQYLVIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            19462..20640
                     /locus_tag="PC472_002069"
     CDS             19462..20640
                     /locus_tag="PC472_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2727395.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            20990..21871
                     /locus_tag="PC472_002070"
     CDS             20990..21871
                     /locus_tag="PC472_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2727396.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            22080..22229
                     /locus_tag="PC472_002071"
     CDS             22080..22229
                     /locus_tag="PC472_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2727397.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(22489..25056)
                     /locus_tag="PC472_002072"
     CDS             complement(22489..25056)
                     /locus_tag="PC472_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2727398.1"
                     /translation="MIYYVLPLEHKQATIEIIGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     FLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPMHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(25059..25664)
                     /locus_tag="PC472_002073"
     CDS             complement(25059..25664)
                     /locus_tag="PC472_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2727399.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTNTALVT
                     RLLINSMLSISYTSRPSDSLEKMLDETMNIFLHGIMNDGGE"
     gene            complement(26038..27498)
                     /locus_tag="PC472_002074"
     CDS             complement(26038..27498)
                     /locus_tag="PC472_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2727400.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            27876..28454
                     /locus_tag="PC472_002075"
     CDS             27876..28454
                     /locus_tag="PC472_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2727401.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            28745..29995
                     /locus_tag="PC472_002076"
     CDS             28745..29995
                     /locus_tag="PC472_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727402.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAVPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            30156..30725
                     /locus_tag="PC472_002077"
     CDS             30156..30725
                     /locus_tag="PC472_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2727403.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT        10449 a         5275 c         3738 g        11468 t
ORIGIN      
        1 gaatataatt ataaaaataa aaaaagaata ggataatttt cctactcttt tttctgtact
       61 tacatataat attaaaactc tttatttttt aatatatatt ttaaattttc ttttttaata
      121 aatctttttc ttttaataaa tctctttaat tttcttcttc tatatcagaa tcaaagggaa
      181 tccatgcatt accaaactct ttatctttaa ataaataagc taatccagtt atttgcttca
      241 atctaagaat ttcatcaact tccataccca ggtgctttga tatccattca tcagattttc
      301 caatttcaca aagttcagtt ataattttac tcattaaatc cacattatga gaacctcttg
      361 cacgattgtg tcgaatagta cttgccattc tttcatcaag agatttatca attactgaaa
      421 caggaagaag tccattttct ctatcatata tatcctcatt ttcaagcatt actctatatc
      481 tatgaaaacc atctacaata atatacttat ccttttcctc attataataa cagacaattg
      541 gcattgtata cccatcttct tttatacttt tatacaacaa ctgcatttct ggtggggcta
      601 ctgcatttgg attatagtcg ttagattcta tcttttcgat tggcactgcg atgattccat
      661 atacaggact tttatattcc atagcatctt aactcctatc taatgaattt tttcatattt
      721 cttcttaata tattccgttc taattctctg ttcttttgta ggaccaaatc ccataaatct
      781 acacatatgg tcatttttta aaatacaata acacattctt ttccaacttg gtatatcttt
      841 ggtaaattta atatcatccg tatcatcagg tatatcatcc aaaaatatga ctctactctt
      901 tttatctttt gtatgatttg aaactccatt taattctatt ttataacctt ttctcaacaa
      961 ttcacgtata actatttctg acaaaccacc acctgtattc tgccaaaact caatagatgt
     1021 tgaaaatttt ttaatatagc tattccttat attttcaggt aaggtatcaa gcaaaaaata
     1081 tgtgaaactt ttccatgtgt gtccatatgg caatttaaca ctacgatatc ccatagcttt
     1141 tgttttgcca taaatagaag taaaattaac tccttgtact ctagcaacca atttcaccca
     1201 tacatctggg tccaatattc tgtataaatt taaactctct tttgctgaat cattaaaagg
     1261 tgatgccact ctcatttttc ttggagctac tccagcttta tagaataagt cataaatttt
     1321 attatagtca tagtcaaatt tatagtgtgc taaccatata tcttctattt cccaatcata
     1381 tataggagat gccgtccaga catttttaaa ttccttagtt atccagcact ttcctttata
     1441 tccatgctgt ttattcacaa tagaacaata acgttgtaga gattctgctg ctcgtgttcc
     1501 taataagcaa acaacttttg caccttcatg tatatctcta atccatctac caaattgttt
     1561 tgccaaactt tcttgtctca ttttatattt ataataagta aaaggattat ttttttgatt
     1621 tattatgtat tctttgactg gcatttccct tacccataac tcttctttct catcatccca
     1681 tggataccaa tacatttcat aatttcctac agcagttctt gtagccattg gtaaacacac
     1741 ccaatatggt tcaatatctt caatattatc ttcaaatact ttttcaacat attcagtagt
     1801 caaactatat tgagcctcaa aatcttgatg aaatactcct attttggaac ttatattatt
     1861 ctttctctta taatccaaaa ctaaatttag cagtaaacca ctatcttttc ctccgctaaa
     1921 agatacatag atataatcaa attcattaaa aagataagaa agtctttctt gaaaagcttc
     1981 aaatacatcg gattctaaat atctcttatc catttgaatt atcccctttc aattagaagt
     2041 ttataaaacc ttacaatgca atattattat gcttttttag cagtaatcag cataaattat
     2101 acattattta aatacacaaa tccaactaaa tacatataat tataatgtaa ttatactttt
     2161 gtattataaa ctatatttta tcattatttt acattttttt ccaatatata taattatcat
     2221 atttttctat tagctaaaac taattacctt atatttttac gtattggtat aataatacat
     2281 aaaaacttga ataatgcata aaaacttgac atactgttgt cataattact ttgctataat
     2341 taaaagtaaa aatggaggta aattctaatg tatgaacgta tgcttgataa aagcaataaa
     2401 ccctgtttgt ctcaaataat agaatatata ggtaaagatg gatatgcacg attgtgtaag
     2461 ttggaaactt acttgaaaac tcaatataat ttatcaagag aacttcgatt tccttttgga
     2521 aacaattatg gttggggtta caaatatagc cataaatctt ttcatttatt ttatgctttt
     2581 tttgaaaaag gtgcatttac tgttctgatt cagataggtg acaagcaagt tccagcagta
     2641 gaaaaaacgt tggtagcttt gacaccaaaa gctaaagagc tctgggtcac tcgttatcct
     2701 tgtggcaata atggaggttg gattaagtat cgtattttag aagatgcaga cttacttgat
     2761 attattcaat atataagagc aaaaaaatct ccacctaaac aaaattaact tttatttttt
     2821 gtgatttaaa tatagtgtat ataaattcca ttctttccat ataaaaaaaa taatataaaa
     2881 aatatctatt agtataactt atttagtatg tcttattcta ctttttacag ataattaaaa
     2941 agttttatca taaataaatc ataactagac atcctagtaa atcaaaatta atttattctt
     3001 ttttattcga atttttgata atagtcccta tacatatgcc aattatcatt ccaaaaatta
     3061 gaccataaca cattgtgata cctacatttc ctttagaaat acctatagca caacctatag
     3121 ccattccaag tatcatgcct ataaacatac atataataac taaattgttg tttttattca
     3181 tagttttctc cattcaatat attttaaatt gcctagctcg ccttcttagc ttaaaacaaa
     3241 gaaatataaa ttaactttct ttcattctaa tatctgcctg tataaatgtc aaacaacaca
     3301 tttcttgtca taacctcctc tttttgaagt aaatataatc tcccagacgc aataatatca
     3361 atttcctaaa accatttaag taacttttat atttacataa tatttacaat cataaactat
     3421 attgttattc atttatacat cataaacact ccctcctaat cattattata cttaaaataa
     3481 tgttcattta taataatata tctaacgcac tttaattgaa aaaactaaaa ctttgccttt
     3541 cattcccata tataaataag tatacttttt gatgctctat tacaaatcaa ctgaattaat
     3601 tgatataaat tatcattttc tgttgaaaat accattcgca catgctatac ttaattgcat
     3661 aaatggtaat aaatgtcata tgattttaca aattattaat aacaattatt acttttactg
     3721 tattttaaat tttggggact aaatatcaag aactttctaa aatacaaagt acaaaaaatt
     3781 tatataagtt ataaatttat atgaactaga taagctcaaa caatatgtta tgtataaact
     3841 gtgtagtatt cggttataaa tttatataaa ttatataagc tagataaagt attacctcaa
     3901 ctatataagc tagatatata aattgcttat ataagctaga taagaattat ttcaactata
     3961 taaactacat atataaatta catacataag ctagatatat aaactagata tataaactgc
     4021 atatataagc tatataagaa ttatttcaac tatataaact acacatgtaa aattcatgta
     4081 aatttactaa tataaatcaa ataaaattac taaactctct aaactaattt tgtaaaacat
     4141 actctatttt taacatttgt taccttatac ataagtacaa acaaaatcta actaaatgaa
     4201 aggataatgt aaaatgaata ttttaaaaat gaaaaaaaca gggatatttt taggaggagt
     4261 actttttggt actgtaggtg ttaagatact ttcaagtgat tgtgttaaaa aaacttgcac
     4321 tcgtgcaaca gcaggagcat tgaaagttaa agattctgtt cttgaaacaa ctacgaaaat
     4381 tcaagaaaat gtagaagata ttttagctga agcaaaagaa atcaacgagc aagaagactt
     4441 atctacatct aaggtaaacg ttgtacaaga tactcttaaa tgcgaacaag aagttcttaa
     4501 aactgaaact ataaaaactg tagaagatac taattctaat agcattaaat gtgaagaaat
     4561 caaagataac gtaattgaag aaaaaaatag tacttgtaca acagaataga tttaatgatt
     4621 attatgatta ataatatcag gaggaagtaa attgaatttt tatataaaac acgaaataag
     4681 aggtcgtata cgctttgact tacaacttgg caaattaaac aacaagcagt ccgatactct
     4741 cttatattat ttaacttcta taaatggtat taccaaagca aaagtttacg atagaactgg
     4801 tgatgcagtc atttattaca ctggtgaaag agaatacata ataagagaaa tcactaaatt
     4861 cagttttaac aatagcaaaa tagaaaacct agttcctgaa cataccagca gggaattaaa
     4921 caattattat agagacaaat tagcaaataa actgctagtc aaaagtatct ccaaaatatt
     4981 ttttccaaag tccttaagat tttttttaat tggacttcat tctataaaat attttaaaaa
     5041 aggtctctct tccctgctaa ataaaaaaat agaagtttct gttttagatg ccactgctat
     5101 aggtatttct atttttagaa aagatatcaa tacggctggt tcagttatgt ttcttcttgg
     5161 tatcggtgaa ctattagaag aatggactag aaaaaaatct atagacgact tggctcaaag
     5221 tatgtcacta aacattgaaa aagtatggtt aagaaaaggt gacactgaaa tcttaattcc
     5281 aatttcagag ataaaggaag gagatttagt aagtgtaact atgggaaata tgattccatt
     5341 ggatggagtt atagtatcag gagaaacaat ggtcaatcag gcatcactta caggtgaatc
     5401 ccttgcagtt aacaaaaaag aaggctctta catatatgct ggaactgtaa tagagcaagg
     5461 caatattgtt atgtgcgtca aggaaaaagc tggtactact agattccaga aaatagtaac
     5521 tatgattgag gaatctgaaa aactaaaatc ttctgtagag agtaaatttg aacatctagc
     5581 agatacatta gttccataca gctttttagg aagtatatta acctatgcaa ttactagaaa
     5641 ccctataaaa tcactctcta tattaatggt agatttctct tgtgcattaa aactctctat
     5701 ccctatttca gtactatcag ccatgagaga atgtaacaat aacaacatta ctgtaaaagg
     5761 tggaaaattc cttgaaggtg ttgcatgtgc agatactatt gtatttgata agacaggcac
     5821 attaacaaaa gctcaaccaa ctgtttcaga tataattaca ttccaaaatt ataataagga
     5881 ggatatgtta agacttgcag cttgcttaga agaacacttt ccacactcca tagcaaatgc
     5941 agtagtttac gaagctgaaa aacaagggct tagtcacaaa gaaatgcata cagaggtaga
     6001 atatattatt gctcatggca tttcaacaaa agttgatgat aaaaaggttg taataggtag
     6061 caaacatttt gtatttgaag atgagaagtg tactatccca gacggtgagg aggataagta
     6121 caacaatcta tcagatgagt attcacacct atttatggca atatcaggta aattatcagc
     6181 agttatttgc ataaatgacc ctttacgaaa agaagcaaaa tatgtaattt caaatttaag
     6241 agaatgcgga ataaaaaaga ttgtgatgat gactggagat agcgaaaaaa cagctaaatc
     6301 tatagcaagt aaagttggtg ttgatgaata ttattcagaa gtcttaccag aagacaaagc
     6361 caattttgtt aaaagtgaaa aactcaaagg tcgcaaagta attatgattg gtgatggaat
     6421 caatgattct cctgctattt cagaatcaga tgtcggaatt gcaatgagtg aaggtgctga
     6481 aatagcaaga gagatttcag atattaccat atcagcagat aatttgaata atttaatcgt
     6541 tttaaagcag attagtaata agcttatgaa aagagttgat ttaagttcta agtttattat
     6601 tggctttaat ttaggtctga ttttacttgg tgtaggtgga tttgttagac caagtacatc
     6661 tgccttttta cacaacacat ctactgtcgg aataagttta aacagtatga caaatttatt
     6721 ggaaaacacc aatacttata actaccatta atctccatta cataaaacat atgatataaa
     6781 aaatttataa ttcatctttt taaaagaata tgtcttaaaa ttagcttttt tctaatcaaa
     6841 attatataaa gcatacttta caaattttaa actcaactta tttctatttt ttacaaaaaa
     6901 ggagatgatt caaattttca ttttgaaaca tccccttttg taaatttctt taaaatttat
     6961 atattaatta tattttcaac caataattgt attttcaacc aatctttctt catagcattt
     7021 taaatcattt tgctttagat aattaaattc agactctaca taaataccac agcctaccaa
     7081 ttcaattgtc aaattaggta tattttcctt tagtatacta gtctgctcat aaataattct
     7141 ttccaataaa tcaacttgta atatagaaaa atgcttcata atctccccat cactatctat
     7201 atctttcttg atttcatacc aactctttga caaaattccc ttcaaatcaa tataccctag
     7261 actcctcaat atatctatac tatcgctcca tgcatactta ctaggtttat caaagtatga
     7321 ttctagtttc ttcttctgct catttgtaag tgaatcataa gaaagcttct tattaaaatg
     7381 tgccttcaaa gaaatcttag ccatagaata tggacttcta ccagagttta atcctgcatt
     7441 tttgaacttt tcaattcctt tatcttctac tgaagaccat attgagttta ttgaatctga
     7501 atcattgtat ttaatatcaa gaccatgtgt aatatttata tagttttgtg gaaatacata
     7561 ttgataatag acatcttttt tatcatcata tagagagaag tatccactat catataaatg
     7621 aatatgcacc tttactttta aactatcctc taaaatattt cgtctaaggt tcgggatatt
     7681 tgcatacatg gttacatatt ttatggcatc aaagttttta tttaatggaa cttcaaaact
     7741 aatttcatct cctgtcaaaa gctttttcac ttcttcttca ttacaatcat atagagaata
     7801 tgtattttca aatcccacac gtgatttccc atctaaactg cctgttaatg ctaccttcaa
     7861 aatatggtta tctgtttttt gccattggta agcatcttgg tcaagaaact ctctaatctg
     7921 tctattttgc tctttcgcct gatttataac ctcgttttca tcttctgtaa tccctacatg
     7981 gcatctattt aagatattta ctatattgct tccaaatggg aatgtccata gttgaaaagc
     8041 tgctgcctca acctgacaca aatatctaac ctgatttaat tcaatttgat ggataagttt
     8101 tttctgatat tcattcatct gattaccact atctgacatt tgataaatca attctcccat
     8161 tgcaagatta tcttttttct tatcaatctt atcagtaatg ataatttgag tttccatcat
     8221 atcctgtact tttttaaata ttatagaaat ctcctctgag ttttccattt ttaagcttcc
     8281 aaacttcgtg cttaattcag ttaaaacttc cttcatcttt aatttactta tctcattttc
     8341 aaaaatatct tgatttttaa ctgtactatc gaatttttct acaaatggta taacagattc
     8401 tttcaaagtt ttatctttaa attccaccat ttgagtattc aattcatcta atttcttaat
     8461 attattttct ttatctttaa ttgcctcttt aactacctct attttactct caaaactaga
     8521 ttttacatta agacaattat taacaccact aatcgcagta tttataagag aacaagcaga
     8581 acttccaata ctcaatgcat ttttaatttt agatgtcttt tgctgtcttt caatagcttg
     8641 tttacttttc tctagtgcag ctaacttatc tttttgctgt ttattattgt aatttgcctc
     8701 caacatacct atttcttttt tctggatttt tgtgtaaatt tccttatact cgctttcact
     8761 taacccctct tttgctcttt gaatatcgtc agctttgtct gcatagttat catcatctat
     8821 agacttaact ataatctcct taatttggtc aaatttagtt cttctttctt catcaacacc
     8881 tgccaaatta taagtcattt tatctaaagc attttttcta tcttcactta gctgttctcc
     8941 aagtacaaat ctcataccat cagaatccaa tttaaaatct gaaaaatcaa ccatcctgtt
     9001 agtccattgg tattcctctt ctttagctga aagattttcc ttcaaagatt ttgcactgtc
     9061 aaatgcccct agacaagctt gaccaacacc actaaatgca gtcagtaaac tcaatgcgct
     9121 ttgtattcca gaaagaatta actgtgtttt catttgttcc tcaagttttg cctgtttctt
     9181 ttttagttcc tctatgtctt cttgttcatc ttctttcatc tttataattt cttgaatcaa
     9241 caggttcatt tttgcattaa cagaaagcat tttattctca agttcatttt ttaaaacctc
     9301 tatttgttga tttccagatt caatttgagt taggatactt tccttatatg tattgaaata
     9361 tccttctttt aactcatctt tctttccttg ttccaattct cttaaggtct tatccagata
     9421 gtctgtcaaa gaacgaatat ctgtaacacg atttcctcca atttcacttt cttcaatcat
     9481 ctgcacataa aatgctgtct tctgctcaag tgcaaataaa atcatactcc attcgctaaa
     9541 attctgattt tttcctacaa aagattctat tctatcagac tgcatcaatt tatttataag
     9601 gtattcatag atttcctttg cctctttata aaattctttc tttatattca tgtcatccat
     9661 agaaagtact gctttatttt tcttctcaaa actatagtac ttctgaagta tttctctatt
     9721 taattgcatc tctattttct caactgcata aaaaatttca taaatatgtg aaaattctaa
     9781 aatatcagct tctaaaattc ctactgcaca caacagtcta agatttctat aacctttaat
     9841 tccagaaaaa ccaaatctct cgaaaaattc atagaagaat gtatctattg acaaagaata
     9901 ctcctgcttt aacaattcct tttcttttag tgcatctcca gagtgaccgg cttttccctc
     9961 tgctccggct gaacctgaat cttcacgttt gctaattgtc cagtccttat gttcttcata
    10021 aagagtattt aatttctctc catcatctgc tgcttgcttc tgatttaaag catgattaaa
    10081 cacatatcta tcaaaataat agtagtttct atcactatta tccattggag gagaggtacc
    10141 tatatatctt attgtttctt catatatacc tctaattcca ccaagacctc ctgcaccacc
    10201 agctcctcca gagcctcctt ttcctgctaa accatgattt ccatcttttt tacttatagc
    10261 actttttaat aaaggagctt ttccaaggag ataaacctgt ccagacttgc ttccaagacc
    10321 tgcatttcca ccattacctc ctgtgcctcc agttccacct tgttttcctt tttctgcttt
    10381 tacaacatag tattttgtac ctttaacatt agcaaattct tttgactctt ttttagaacc
    10441 tattttagca tttgccccag cttctccagt tatccctgta tctccttttt ttccatcttg
    10501 tccatttcca ccacgtccac catctacgta aagtttaagt tctcctcttt tttctatttc
    10561 tttaaaagat aatatgatat cacctgaagt acctgcatta ttgccatttc ttccattcat
    10621 tccgtcgtaa atagaacttt ctccaaccaa tttttctgca tccttaccag atacgttgat
    10681 tgaataagtc ccttctggta tatgtaattt ttctgtaagt agtacaatat tcttacctga
    10741 gaagacttct ttctccatat tacagtcaat tttcaactca caatctccat acatatatat
    10801 agatgacaaa atattaagtt gataaccaga tattatttta gtcaaatccg ataaattcac
    10861 tttaggtata ggaatatata tagaaagtac attttctttc tcatatgatt ttatatatgt
    10921 acaactttga atcttctcta tcctatactc tgcatcttca agctttttgg cttcttcttc
    10981 cctatattct tcttctgcta tccaatattt gatatctttt aatgtatctt ctaaagtatt
    11041 ttttagcaat gattttaaca actcacttag accaacaata tgagtctcac tacagtaact
    11101 atttaactct aaaagaagac gtgtttcttc taatatattc actttctgta cgccatcacc
    11161 atatgaaagt tcttgtacaa actccataag ctctttcata ttattagtcc tactacataa
    11221 atctgtatac atcttatcta attgaatctt tttatctttt agactattta tagaagatag
    11281 aatggaaata gaatcttcca tacctttttt aacactttct atataaggaa ctaaaagttt
    11341 ttctaggtct ttataggtta catcaacagc tttttgtatt tctattttac aagaagctgt
    11401 aagcatcaat cccaaatctg ttttttcaaa tgtttcaggt agatttttca tttcatccaa
    11461 caacttttct ggctgtttat ctagtggatt acataattta atagatacac catctgataa
    11521 tgctacattt aataagtaca ctaattcctt acaaccgtcg taatctttct cttgactatc
    11581 taagtcttct tttgcttttt ccaatatatt tcttattttt tcttctgtta cattaccgat
    11641 tagtgtttta ggtattttat taataagaaa gaagaaattt tctttatact ttgcataatc
    11701 accaagcata tctttcaaat ttttgcctgt ttctatcaat aatgtatgtc tagagtccag
    11761 tagggattga tagtctaaga taactaccat ccctttcacc tttgaagatg tggaaatagt
    11821 ttcccttaca agcatagcac cagcaatcat atactctgct ccacggttgt caaaaaaacc
    11881 tgggcaatca cataatgaaa aatctctatt tgcaatatct acaacttctg gacataaagt
    11941 cttagaatat acaccatttc caatttcgac agctgaatct tttgcagtta tatatatttg
    12001 tccagtctct tcatccgttt tttcttccat cacacagcct tttaaataat ttatacaagt
    12061 actctttcca acgcctgaag cacctaggaa taacaaaatt gacttttctt ttaagtcata
    12121 ttttctaagc tcgtctcttc cttcttccaa aaggtttacg atttcactat aaagtttatt
    12181 tgccatacat ccatcccttt ctatatttcg gaacaatagc cttgatattt ttctttaata
    12241 agattctctt ctacctttga aacagctgtt gataacttag tatttggagc attaaatcct
    12301 ccattgtctc ctctatctcc atattcagcg tattctcttg ctggtcctgt tttaccttca
    12361 ttccagcttc caccactaaa ttctatatga tatttaatta tactatctaa cttataatcc
    12421 caacttgtaa aatatcctgt atagtataca gtcttatcta taccacttgg agaaaatgaa
    12481 agtgtattat atccgtgata gccaccactt cctccatatc cacctaaacc tggtctacca
    12541 tttggacctg cttctccatc tttcactgtt atatcacaat gattaattat tctatttttt
    12601 tgttcctctg aatctgaaat gaagtaaata ttacctttct taccagcatt tccacctctt
    12661 ccagcatttc caccagctcc acctttttca ccttttttgc cacttgtacc tttttcaaaa
    12721 taccctttaa acatctgtcc gtggaaataa cttgtatatc ctgcatcagc tccattactt
    12781 ccagaagctc ctgtttcacc attttctcca tctattcctt tagaaccatt tcctccattt
    12841 gcaatcagtg ttagacgata atttctaatt tcttctttaa tatataagta aatattacct
    12901 gcatctctac catcttcacc atttttccca tacatagaat tatctacaag ttctccgctc
    12961 tttccactta cattaatttt tacattacta cctacatcaa gattgttaga agcaagaata
    13021 atatttttct ctgatgcaaa gttcattcca aggttatcat ccatataaat agtatcttta
    13081 gcaaagaaca taaaagtaga aacattgtca tctacttttc tatttttgaa atattccaat
    13141 acactgctaa ttactaagtt tggaattaat gctcttattt caataccttt cttatcatta
    13201 atattaattg tttttactat aaatggattt aaaatgtatt taccaataaa ttctctaatt
    13261 atctcattac cttcttctcc atatgcactt aatcttgtta tactgtcttc taactcttca
    13321 ttttgtgtaa ctgtaacatt tgtcaaaatt gaattaaaat cacttacact atatacataa
    13381 tcctttttat ttgcaacttc cattaatttt tctctatttg gtaatgtaaa tacctgtttt
    13441 tgcacttcat aagaaactaa aactttttct aaattctttc ttattataat gaagttttgt
    13501 tccattatct tcttacttgc cttttctata ataaattcca tatgtacaga aggtaataaa
    13561 atttccccag ttccatgttc tgtatattta cttaaagcat caattatatc taagtttcct
    13621 tttatcttat tttgtaattt catgcaacta tcttgtaaga ataatccact ttgtttggct
    13681 aatacgttga taaatctctc tatagtaaga cttgttgtac tttccaacca gtttttaaat
    13741 acttttacaa cctcatcata ttttgctatt tcttgaatca ttgttatact tccaataata
    13801 ttgataaaat cttctgtata cgccttaaca taatcttcta ggctagatac gatattgttt
    13861 tgaatttctt ttatcaacgt atctaaaact aatcttgcat ctgcactaat tggatattgg
    13921 aatgctgatg tcttatcttt aacttcatct aattctagta cttgctttaa taagccttcc
    13981 ctctcatctg atgcaagtgg cttacaaaga tgaatatttt ttccattatc ttttagcaat
    14041 tcttcaaata accaagcttt ttcctctata ttactgtcta aattttcgca taattgttca
    14101 tattttcttt ttataacatt atatatttgt ttttctgttc ttcctaagaa actttttgaa
    14161 atgaaaaaag ttatactacc cttaaaattt tctttatgaa gacacatatt taactgttgg
    14221 aataggtcta gtacttttgt aagtctagaa tctaatatat ctgcttctgg tatcaataca
    14281 attactgcac atatagatgc agaattttta aatacttcac ctaatgaaac tgctgcacta
    14341 atatcatggg ctactcccct attgtcgcta aaacctgcag tatcacataa ttttgcatcc
    14401 attccttcta ttggttttct catatttaca atgttcgggt ataaagtttg agaaacagct
    14461 cctgaaccaa tctctgccac agggtctaag caatcaatat atgatatacc agtatcttca
    14521 tcttctactt ccatcattac acatcctgca agatagttaa ttgcagtact tttgccacat
    14581 cctgtcccac caagaattag aattaaattt tgattttcag ccttatttat tgctactttt
    14641 ccttcattta ataagagtgt tatttcttct aaaagttgat tgttattttc tggcatttat
    14701 tttccccctt ggttatatat tttatttaaa taaagtttaa tcccttaagt agattgcaaa
    14761 gtttttagac tatatttaaa tttgtttttt attttttatt tttaataaat ttgctttttc
    14821 actaaatttg ctttttcact caataattat tcttctttgt ttattaaaat aactgtgttg
    14881 tataatataa tctttaccaa cacagttatt ttatttaatg cgcttatttt attgtatttt
    14941 atgtaataca gttgtttttt atattatatg tccattattt tcctgtggtt attacatata
    15001 gatttttaat tatatttgta ccattaattt aaacattcct atactactgc ttaagtttta
    15061 cacctatacc agcttgaata ttttattttt atttgcttaa atagttcaac tatactggtt
    15121 taaataggtt atctttattt gcttaaatag ttcaactata ctggtttaaa taggttatct
    15181 ttatttgctt aaataagttc aactatactg gtttaaatag gttatcttta tttgcttaaa
    15241 taagttcaac tatactggtt taaataggtt tcctttagtt taaataattc acctatacct
    15301 acttgaatag ttcactttta tttgcttaaa ttctatacca ataccggctt aaacattata
    15361 ctaccatgat tgtattgtct atcttcaact acaataaaaa caggttctaa atctttagta
    15421 gatgttattt ttaattctaa aaaaatatta tcagttggta atatttttac atcataatta
    15481 tttaaattat tattcgtgat actattaaat acatcacctt gacgttcaaa aaaaacatta
    15541 tcaactatta caaaataaac atcttctttt ctactatttt caacctctat agtattttgt
    15601 ttagttacat ttagtctcat cttccacctc ctattaatat tacttaatta tattaatact
    15661 tgtaattgtt gcattatgtt catcacaagg aagaggttta aaaacaacta tctctgctaa
    15721 ataattctgt ggaggatttg taactgaagt agcattgcaa ataaatttaa aatttgttcc
    15781 tgctacaact tgagtagaaa caataagtgg ttcataacca accccttcta acattcctac
    15841 agcttcatta aatactttta aatcttcttc ttcgacagct ctgaattttg accatccacc
    15901 aacaaaaaga ctcatataaa aacctccttt atatatatat ttatttatat atagtagtac
    15961 caattttctc aatattcaat attttatgtg taactggtca atgaaaatgc ataactggat
    16021 aaaaatactt ataaaataac ttaactttac atataaatag cttactttac atgtaataag
    16081 attattctac aatatgaaat gcatcttgtt ttgaatatat tctaaataca ataatggata
    16141 ttttcaatta ttataattag aaaatatcca ttacttaact tgttttattt aaaatcacat
    16201 agagacttta ttattttttt tattgcatga atagctattt caggtatcat aaggtgatat
    16261 accccctcct tgaaaccaca aaaaacatga caaactttac gaatcttggc ttaaaagacc
    16321 acaggtacat tccttatttg tggattggag tagccataac aagcaaattt tacaattttt
    16381 atgatgacaa aaatcaataa aatcgcttag tatataacac gaaacactac ttgttactat
    16441 taagaatagt tagataggaa gtatgcatta taccaataaa ggaatcaata aagaaagtta
    16501 aaacatccct acaaattaaa aatctgttac atttttctat atttaaacgt tatttactta
    16561 caattggaga aataacaggt tttccttctc ggtcaagtaa aggcgtaaat ccggctgcat
    16621 tgccataata gtggaataca tagttcacac ctgtttcttt atccacccaa atctccgttg
    16681 catttatttt tccttgtgaa tatacttttt caaatctatc ttttcccatt ttataatccc
    16741 ccatattttt gattggtcta taccttcttc acttaagtcg aaaaaataca taacttattt
    16801 ttttaattct aatatgagca cgtaaaaatg tcaaacagct tatttaattt cataacttca
    16861 tgttactgtt cgtaaccttg tctgtttact tacactgcat tacagcacca ttttatcctt
    16921 tacattattg tattgcacac agtaaaaatg cttactttat gggatttttc aactaaaaaa
    16981 aggattttat ctccacaaaa catttactat ttgttacaat atacctaatt ttaatctcat
    17041 atcaacattt aatgaagata taaccttttt caaatattgt aaagactcct taaaaatatt
    17101 agaaaaagat tctgcaaatc tctcaacatc tccttttttt aaatacctaa gtatctcaaa
    17161 acatacatca aaacgtgttt tcaaaatttc attatattct tcatgaaaaa atctctctaa
    17221 atagattcca aatatcatat attgttttaa atgcaaaaat gtttcctgta atacataaat
    17281 ttgtgtataa tctactatct tacaaagtaa aacaaaatct ggataaacct tacactcttc
    17341 acaattattt tgtaagtacc ttaaatgagt ttctatttta tctacagcta tcaaatccat
    17401 tttatttcct agtaaaagta ctacatcctc aagagtaatc gtaacaaact ccatagcatc
    17461 caaaagcaaa ctcaaccttt tagctccttc ttctgtattt atataatcat catcatattc
    17521 agaaaaaagt gttatatatg ttcccttgcc attaattgtt ttagcaattc ccaattcatt
    17581 tagctcatta taggcacttc taactgttgg aagagataca ttatatctat taattatcga
    17641 agtaattgat ggtagacaat ctcctatttt aatattgcct tcaaaaattt gacaaattac
    17701 atctgaaaca attaagtcat ataaataaca tttttccaac gttacagaat taaaactact
    17761 tgtatctata ctttgtggag cacgattttc taccatttta agtatggatt ctctacaagc
    17821 tttatacata tatgttaata gttttttaaa ttcaattaga ttcccgtcca atccatataa
    17881 gtaagttttt cttacctgct ctatgtattt attacgaaaa aagtctatac aacaattatc
    17941 caagttatcg aaattatagg ccaattgtga aataatgaca tcatgctgaa taatctcaca
    18001 aaaatggtga atcaggaaat tatttgtttt acttattagt ttatttatat attttagcct
    18061 ttgttcttta aagactaaat aattagaact atttttttcc atacaatcga taatctcgtt
    18121 taattcttca aagtctttag atgtaaatct tttaatattg tacatagcaa tagaaggaaa
    18181 tattaaacta aaaatcttat atatttctga tatttgtaaa atcttctcat ataataagtc
    18241 agattctatt gattgtttac tcgtactctg gattaaaact ggtctagaac cagcttttgt
    18301 ctctataaag cccttttctg agagcatttt tattacagtt tttatagtat tttttgatac
    18361 catatattct tctgacatgc tttttataga atcaagccta tctccaatag taattttacc
    18421 agtataaatt tgttgaatta tatgctcaaa aataactaaa tattgtggtt ttctcttcat
    18481 atttttcctc ttcgataaat tagatttatt gttttttgta aggtatattt acttttattt
    18541 aatagggcaa aatagttgct tattttttcc tcataatcta taattaatat aaaccattta
    18601 tatatatggt ctaagtttta taatactgta atgcagtaaa atagcatatt gtagctatac
    18661 tacagcaaat taacatacca ttgtagtata ttaattggaa taattttaag tacttcaaaa
    18721 ttccatcaaa acatatatgt gtttttgata ttatgtatat tcgttaatag aatagctata
    18781 cttgttttac ataataaatt atatgatact tgctatatgt aattactata tttaatacac
    18841 ataactatta tacttactat atgtaattac tatatttgat acacataatt atgatactta
    18901 ctatatataa ttactatact tggacacata actattatac ttgctatatg taattactat
    18961 actttataca catagttgtt atacatgcta tatataatta ctacacttgg acacataact
    19021 actatattta ctatatgtaa ttatactata cttggacata tagttatcat acatactata
    19081 tgtaattact atacttggac acattattat atttgctata tgtaattata ctatacttgg
    19141 acatatagtt atcatacata ctatatataa ttactatact tggacacatt atcatacata
    19201 ctatatgtaa ttactatact tatacacata gttatcatat atactatata taattactat
    19261 acttggacac atagttatca tacatactgt atattaagat ataatacttt ttaaatttta
    19321 tataacacag acacatgcaa taaaaaagaa ggtatccata ataagctctt tgcatgtacc
    19381 taaataaaaa ctgtaaaata gaatatataa aatctaatct attttaagta aatacaattt
    19441 cactttaaaa gaggatgtac tatgaaatac catatatttt taatcataac tactatatta
    19501 ataatcgaac tatgtaacat ttacatatgc aaaatagcat cttttggaaa caaaaatata
    19561 actatacata agaattacaa atatattttt ttaataaaac cattaacctt cttgataata
    19621 atattcacaa aattaccatt accactatat tatatattgt tttacatagt aagtcataaa
    19681 ttgctcttaa gcagaaaaag ttctactaac aaccatttta ttcccaatat ggcagtaata
    19741 agtttttgta ttttacacct tattactgta agtacaatat cacttgttaa aaatacaagt
    19801 ccagatataa tatttgcaaa tatgacctat aagttaatta gtattttaat agcttctatt
    19861 ttagatttta tcttatggta ctctatgaat aaaataaaag agcatattta tataataact
    19921 caaaatcaaa tagagttcag tctattttta aaatttatct ggttcacttt aggatatata
    19981 ttatttgata gtatacaaat tacatttgct ccaccaagtt ctctaacttc ctactttata
    20041 ttgggaagta acatattatt aatcttacaa ctaattatat tcatcaatca tatatattcc
    20101 attgaaaaaa atatttattt agaaaaagaa cataatagac taaaaatgat aaaacaagaa
    20161 caattactaa aagtaaattt attaaagaaa aaagctaaca ttgatgaatt aacatcagca
    20221 tataatcgaa aatttgcttt tgaaaaaata cagcattaca ttgataacaa tttattattt
    20281 tcattgatat atatcgattt ggataaatta aagacgataa atgatacata tggacattta
    20341 gctggggata actatattat taacttctca agtatagtta ttgaaaactt aggaatagat
    20401 gacatatttg ctagaatagg aggagatgag tttatagtta ttatgcctaa atctaatgaa
    20461 aacaccacaa aagaatttat agagaaaata agtaaaatta tggataatac gatatctttt
    20521 agctatggta ttgtagaagt ttctagcgaa aacaaaatga ctgtagaaga aattattaat
    20581 attgctgata ttagaatgta cactaataaa caaaataatc ataaaaggaa caataactaa
    20641 aatgaatatt tacatgagct tacttgtagt ctcactatgt tttttatctt attatctcta
    20701 tcattataat ttttttaaat atctggacat aatgattaat aactctaaaa ataaatacac
    20761 agtatatatt atatattcta tatttaatta tttaattttt attatttgta cttatttaaa
    20821 gtttcatcta attataaatt ggacaatttt tatgttgatt ttattcatac aaattaaagt
    20881 aacttataaa atctccacaa taaaaaacat tttttattca ctaaactgct ccatcattgg
    20941 tttagcaata aatatatttt ttaggtcttt aatgtcaata gtgttaaata tgccagcata
    21001 tttatttgat agtaattcta ttgcaaatat ttacagagta tttcctatat catttagctt
    21061 tatagttaca ggactatatt tcagttataa aaataataaa aataataaga ttaactttga
    21121 ggcagtttta gaggacaaaa aaaacttaat tttcttattg agccttatgt ttggaatgta
    21181 cttgtatcta tgtttgagtt tgtcaatata ctatactgtt ggaaatgtat tgattttaaa
    21241 attatatgga atcaagtctg ccataatagt tttggtaggt aactatattg cagaaagata
    21301 tagctttaga attactcaaa taaaaggtta tgatattaaa aatcaaatag aatatgtaga
    21361 aagtataaag acagagtatg aaattaataa attgaatgat attgtatata cagatttact
    21421 gacaggcttc tacaatagac cttttgtaaa tcaaaaacta tcagagtgga catcaaatca
    21481 ctacaaattt accttatgtt ttactgattt aaatagctta aaacctgtaa atgataaatt
    21541 tggacaccaa tttggtgata aatacatatc cactactgct caaataataa aaaaacttat
    21601 aaatgtagaa aattccttac tatttagata tggtggagat gaatttttat tccttttaca
    21661 gaatacaagt tgtgtagaag cagaaaagat aatgattaat ataaattatg aattatcaaa
    21721 attaagtaat actgacgagt atccttatgc tctttccata agctatggat tagttgagtg
    21781 ggatggaata tccgatattg aaactttaat atcaaaggca gattctttga tgtatgaaaa
    21841 taagcttaaa aataaatcta ctttaaacta gctagttaag atattctaca aaatatatgt
    21901 tataagagag tactaagaat gtcaaaatat atagattttg caaatattga atacagttta
    21961 ctaatgaatg cactggatat aagtgttagt aaacacctat tagatgaaca ctttactgtt
    22021 gtttgggcta atgactgtta ttatgaaatg tttggctata cctagctaaa aagttaaaaa
    22081 tgaccactgt agccgaggga atagagacca gttttcaaaa agatttcttg caagaaatag
    22141 actgtgatat gttacaagga tacgtgttct ctagaccttt accaataaaa gactttgaaa
    22201 aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat aagtgatatt
    22261 attgtatcaa caaataaagg aattgtctca aaattgattt tttctaatca aatttttatt
    22321 ttgagacatt ctcatttttt tgcataagaa aatccccacc aagctacggt ttttcttacc
    22381 gctgttcggt gggtattagt agtattatga aatctgaaaa ctttaagtat tactacttcc
    22441 gctagcgcaa tcacaaaggt cgaaagcttc aaagcgaaaa taggcttttt actcatcaaa
    22501 cgtcaccgta cctgccaacc cgtccaccgt tatcatttgt ccgctcttaa tagttcgtgt
    22561 ggcaatgtgt gtagccataa cggctgggat gccatattca cgtgcgacaa tgcttgaatg
    22621 gctaagagga ccaccaatgt ccgttacaat cgcacttgca gatgaaaata atggtgtcca
    22681 tgctggagtt gtggtaactg caaccaaaac acttccaggt tgaaagcttt caaaatctgc
    22741 tggactattc agtacacaag ctggagctgt cacaacacca gtactggtac caatgcccgt
    22801 caacacaatt ttcccatctt tctgcttctg ttgtgcttgt gatatagttt ttttgttttt
    22861 ttctggcaac ttggatggtg acacataacc catgtatttt ttaagttcag cttttcgaac
    22921 aggtattatc ccgttcatat cagatagaga cttgttttta tccaactgct ctataagtac
    22981 ctccaattcc gattttgtga gccaataaat atcatctaaa tgtgaaatag ctcctcctcg
    23041 tatgaatcgt tctgagattt cctgaaacat acaacgaatc agaggatgtc ccattcccat
    23101 caaataaata gcattttcac gcataggaga ggtttcctgt gcccaatgga gcaacttaaa
    23161 aaatagtttt ttacgcaatc cacctatgtg ttgtaaaatc tcttcttctg catgttttct
    23221 acgcttttca aacgtggtct ggcgtaaaaa aggactttcc ccttttcctt ccacaaatgt
    23281 ttttacggat tcaaaagttg gtgtaagtgt ttcctgcggt gtggaatgcg caaaatcaaa
    23341 ctcataagcg gtacgaccaa actctttaaa gtacttattg attcgatttt tccattctat
    23401 ccatacttcc tgtgaaactc caacaggcac aactgaagac atgaaatctt ctgctatctt
    23461 tattgcagaa ctgttttgca gataaaggtt gatgctgtta ttttgcttta cccattctga
    23521 aataccccac aactcctttt ccgattgcag aacaatcgta tcaaacccaa gcaggaaaac
    23581 ggaagtatcc gttataccag cacgtcgaac tgctccttga aataattttg taaataatgt
    23641 ttcactgata gaagcagcag gcaatgtaag ctgaatcctt gtgaaataaa tacatgcggc
    23701 ataaaatatt gtctgaatac cctccataat ttgatgggcg ttcatagtat gcatgggctt
    23761 ttcttcccat tgtttaatag catcctcata tccttttcgt gccgtttccc agcgtgcaac
    23821 gctgttcata agtgtcctac gtaaagagcg tggggaaagg gatttgatag caatcaaaaa
    23881 tggctttgat ttagcagaaa gataaacata tccattaata accgtatatg ccccgttttt
    23941 tggtaataat tttttcgcac ttttaccaaa catatctatc cataaaagct ctgatgcacg
    24001 gtttacgatt tcaagaccaa gcgtggcgaa caaaggtgta acaggatttg gcaagtgttc
    24061 tgccaaactt ccctttgtgt atacaacatc ctgttctggc aatacccatt ctggtggcag
    24121 aacagtaatt ggacgagcct gaacaatgta caatttatct ttttccagtg cccactctac
    24181 atccataggc atttgataat acttctcaat tttctttcca agctgtatca gttgcattac
    24241 ctgattacga gtaagagcat atttttttct caacctttca ggaaccatgg tttcttctgt
    24301 tccttctgaa gtacgaaccg tcataatctc tttatttgcc acttcatacg atataattcg
    24361 ttcggaatcc ttatccacca cgattgtatc gggtgtaact aagctagaaa ccaccgcttc
    24421 accaagcccc catgcagcgt taataatcat ttcgctacgt ctaccattga ttgggtttaa
    24481 cgtaaacata ataccagatg aatcggaaaa tgcaagcttt tgtactacaa cagcaagtgc
    24541 gacaatctcg tgctttatat tattcttcac acgataagca atagcacgag ctgtccataa
    24601 ggaagcccag catctcttta cagcgtcaat aactttatct tcaccttgta tgttaagata
    24661 agtttcctgc tgacctgcaa aagaagcgtc aggtaaatct tcagcagtcg cagacgaacg
    24721 gacagccacc gatatgttcc ctaatccggc ataagccgtt ttaattgcat ccgacacttc
    24781 ctgtggcatt tctccattat ggaaaagctc ccctattttc ttagatacat cttcaagttg
    24841 gctggtatta ttggaatcaa taccatctag cagtttatta attcgagact gaatatggtt
    24901 cgtctcaaca aaaatctggt atgaagcagt cgtaacatga aaaccgtctg gcacagggat
    24961 gcctgctgtc aatagttttg ataaagacat cccttttcca ccaataattt caatggttgc
    25021 ttgtttatgt tccagtggca agacataata aatcataatc attcacctcc atcattcata
    25081 attccgtgca gaaatatatt cattgtttca tccaacattt tttccaaact atccgatggt
    25141 cttgacgtgt agctgattga taacatggag ttaattaaca aacgtgtcac aagagcggta
    25201 ttcgttttgc gaaagatgcc tgtggtcaca ccattatcaa ttaccgattt tataatatct
    25261 tgatatgcat aacgagcatc tttaagtctc tttcgatact cttcttctaa ataatcagat
    25321 tctgtattta accacatcag tacagttcta tactgctctg gaaatctaag atgattaagc
    25381 atgatttttc taaggcactg ttgtggtgaa ggttcaccat caataatcct atgaacttgt
    25441 tggatagttt tttctatttc tttctcaact gcatatacaa caatctcgtc tttcgttttg
    25501 aaaaaatcgt atagacttga ctttcccatg ttggctaaaa ctgcaatttc tctcattgat
    25561 gttttttgga atccattttc agccatgaga ctcaaagcaa cagcaactat tttttctcgg
    25621 cgttctgctt gttgttcggg tgtaagcaca attccttttg gcatagggcg atacctcctt
    25681 ttagcgacca ctggtcgttt cttttttatt tatcatacag cgaccaacgg tcacttgtca
    25741 aggttaattt ttttcattgt atatggtact cgagataaaa atgttcattt tagaaattgc
    25801 tctatttaag tagaaatagg cttcatcatt agaaaacaag actataaatt tgagatattt
    25861 atcatgttca aatttcatca atatcaaagt ttaagatatt aataaaaaaa gaattactaa
    25921 gaaataacat ataaagattc aaaaaaggag ttgaacttaa aattcaatta caaaaaatca
    25981 aatttaagac aactccttta ttctttatct ctactattct ttatctctat ttatttatta
    26041 caactcccta acatttacca ctactgcctt aggtctagtc atagcctcaa ttgaatactt
    26101 aacaccttga gttcccatac cagatgcctt tacacctaaa aatgggaagt ggtctggacc
    26161 tctttcagtc ttattattta tttgaacagt accaacttca agtttatcag ctatataaaa
    26221 tgccttatca atatcacttg taaatactga tgattgaagc ccatactctg attgattagc
    26281 aatttcaatt gcttgattta tatctttaac tcttataatt ggaagtactg gaccaaatgg
    26341 ttcttcccaa gcaattttca tatcaacatt tacattatct aacaatgttg gatagaataa
    26401 attatttttt ctaacattgc ctgttattaa ctttgctcct ttatgtaatg catcatcaac
    26461 caacccttga acaaaatcag ttgctttatt atctataaga ggagtgataa ctacttcatc
    26521 cattggattt ccaattgtca acttctctac caatggcttt atttttccga ctaatttatc
    26581 cgccacactc tcttgtacta aaattctctt tactgccgta catctttgtc ctgaatatga
    26641 atacgctcca gatactatat tcttagctgc aaaatctaag tctgcatctt ctaatactat
    26701 agccgcatct tttccaccaa gctccaataa taatggtacc attccagata ttttagatat
    26761 atgctgacca acttcagtac taccagtaaa gttaataaaa ttaattcctt tatgcgttac
    26821 aacataatcc cctatctcac tacccctacc tgtaatagta ttaagtactc catctggtat
    26881 cccagctttt ctcataattt ctaccacatg aagtgcgcta atagcccctt gtgtagcagg
    26941 ttttaatacg actgcatttc caccaataag tgctggagca atctttgaca ttgataaatt
    27001 tacaggataa ttaaaaggag atatagctaa aacagtacct aatggaaccc ttgaaacata
    27061 agacatctta tttcttgttc caccagggaa attatctcca ctaatggctt ctccctccat
    27121 attttttccc acatcagctg tgtatcttaa aaaatctgct gttctctcaa cttctgccct
    27181 tgcagatttt atgtctttgg ctatttcaag taccagtata ttagctatct catctatatt
    27241 ttctaacaat aaatcagcag ccttatgaaa tatattgcct ctcttgtata caggcatttc
    27301 agcccatact tttatactct cttttgtgtt ttttataacc tcatctacct catgttttgt
    27361 catagattgt actttcccaa caagagaatt atctattggt gagtaaattt ctattggctt
    27421 tctcgaatta gattctaccc attttccatt tattaaattt ttatatacat tttcttttgt
    27481 tttaagttca ttaaacatat ttttattcct ctctctgata taactttgtg tatttaaatt
    27541 atagccattt attcgttatg taaacatttc tttttttcat aaacgtttac ctataaaaaa
    27601 attttaagtt aacgtttttc tacattcttc tattttaata acatactcgc tccaaatcta
    27661 tataggtcac actttaatac taatatatca atataccaat ataccaatat accaatatac
    27721 caatatacca atataccaat ataccaatat accaatataa atattataaa cataaatcac
    27781 ttgacaaatt tataagtaga caatattata tatctatgac tcaaaatatt tttaaagtca
    27841 tatatatcat atatagacag aaaggattga taaaaatggc taaaagtttt acagaaatag
    27901 aaaaagacaa tataagaaaa agactaattt cagaatgtga aatcaactgg tctaaatatg
    27961 gatacaaaaa aactaatatt gatgaacttt gttcaaaatc aggaattgca aagggttctt
    28021 tctatacatt ttttgattca aaagaaaagt tattttttga agtattaaat ggaatccaaa
    28081 acagacttat atcatcagta gatgaaattt taagtaacat gcctccaaaa gaaggctttg
    28141 caaaatcctt aaaaatgtta tatcgattat atgataaaaa tccattttta tattcaccaa
    28201 ataatcctga tttcatcagc ctaataaata aactacctca agatatgttt ttacaacttg
    28261 agtccaataa catacaaagt ttttacgact taattgagag acacaatctc aagttgaaga
    28321 tagaaaaaga aaaagttttt ggtgtttgta atgctctact atcaagtctg ttggtaaaag
    28381 aaaatattga atataactat attgaagtgt ttgactttat ggtggataat cttgtagaac
    28441 acatacttga ataacaattt aaaataattt ttctaagtat ttttttatac taaataggat
    28501 taatatgatt gaattaatgc aaaaaataaa caatataatt tataaaataa atgataaact
    28561 aaatatcaca caaaacttat agaccctaga gcaaacccat tccactctac tctaagccat
    28621 ctataatagt ttgtatattt ctaataatat tagatattaa agtaagaaaa aaattcctta
    28681 taataattgc tattatatca attattgtaa atgttttaat aatcttagat aggtaggtac
    28741 atatatggta aaatcctcga acacttttaa taaaaacttc aacttgatga tattaggtca
    28801 aataatctca ttgtttggtg catcaatact taaatttgct ttatctttat acatacttga
    28861 tataactggt aaagcagaaa tatttgctac tatactagca gtatcttcaa tcccaattat
    28921 agtattctcc cctattggtg gagctattgc agatatattc aacagaagaa acttaatggt
    28981 tatttttgat ttttcaagta gtcttacagt actaatctta gcagtattct tattcaacaa
    29041 taatggttca atattacttg taggaattat tatgaccata ttatcagtca taagcacaat
    29101 gtatcaacca actgttcagt caagtacacc ccttttagtt gataatgagc atttaatgaa
    29161 tgcaaatggt attgtagctg gagttgcatc tcttacaaat attgcagtac ctgtattagg
    29221 aggtgtcttg tatggtgtga ttggaattaa tgcaattata gttataagtt gtgtaagttt
    29281 ctttctatct gcaattatgg agatatttat acagattcct tttatcaaac aagagcaaaa
    29341 cagcaacatt ataaaaacta tatttactga tataaaggat ggtattgttc atatatgtaa
    29401 agaaaataga tttatactaa aaatactttt tttagctata gcaataaact tatttttaag
    29461 ctctatgtta atagttggta tcccctatat agtaaaaatc actatgggag ctagtaactt
    29521 cttatatggt ctagcagaag gagcaatttc ttttagttca ctggtagctg ctgttagtat
    29581 tggtattttc tcaaaacatc taaaaatcac taatttgcat atttccttta ttatatgtgc
    29641 agttatgttc ataccaatgg cggtttcagt atatccattt atattaaaca caggatttcc
    29701 agttccattt ttaatcttta tagtatcatc tatgctaata ttttttacaa cgactcttat
    29761 ctcaatatat gttataacag tcacacaatt aaaaactcca aatgaactct taggaaaagt
    29821 aatgtcaatt ctttttgcag gaacagcaat tgcaatgccc cttggtcagg ttatgtatgg
    29881 taaacttttt gatttattat cagatagagt gtacatcatt atactaggag ttggatttat
    29941 tacattactt atagcatttg tatcaaagat aacattgagg aacgaaaatc tttagataca
    30001 aagaatttag atataaagat ttaattttca ctaatttctg aatttaaatg caaaattgtg
    30061 tatcagtatg ttgacgtttt agaaattgat tgatataatc aatggaaaat aaactttata
    30121 aaaattcaaa atattcagct agaggagtga taaaaatgag ttacatgcca actttagaac
    30181 aagcatggga aatattaaga aaatacaata aggaagaatt tcatataaga catgctcaaa
    30241 ttgtatctgg agttatgaga tactatgcaa aggagtacga ccctgaaaga gtagaatttt
    30301 gggaaatagt tggactttta catgatttag attttgaaat gtatcctgat gaacattgtg
    30361 ttaaacaaag agaattaatg acagagcttg atttagataa atcaataatc gattctacaa
    30421 taagtcatgg atatggatta actggttcag atgttaaacc agagcaattt atggagaaag
    30481 tattatttgc tgtggatgaa cttactggtt taattggtgc tgctgcaata atgcgtcctt
    30541 caaagagcgt ttctgattta gaattaaaat ctgttaagaa gaaatttaaa gataaatcat
    30601 ttgctgctgg ctgttctcga gacgttataa gaagtggtgc agaaatgctt ggatgggaac
    30661 ttgataagct tatccaaaca actatagaag ctatgagaag cttgattcca gagatggcaa
    30721 tatagctcct ccatacatat accatagaag ttacatcttt aaagagcatt tctctttttg
    30781 atgcaacttc tttttatatt ttgtagaata aaaagtttaa tattttaaac tcactatata
    30841 ataaataata taatgtataa tagtcataaa ctttctaacc ttaaaagttt ctaataatca
    30901 ataaacaatt ctaatataca aatgtcatta
//
LOCUS       DAMJYC010000043        30645 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJYC010000043 DAMJYC010000000
VERSION     DAMJYC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.044"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            110..2011
                     /locus_tag="PC472_002078"
     CDS             110..2011
                     /locus_tag="PC472_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2727404.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2014..3057
                     /gene="ypdE"
                     /locus_tag="PC472_002079"
     CDS             2014..3057
                     /gene="ypdE"
                     /locus_tag="PC472_002079"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2727405.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3072..3377
                     /locus_tag="PC472_002080"
     CDS             3072..3377
                     /locus_tag="PC472_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2727406.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3474..3809
                     /locus_tag="PC472_002081"
     CDS             3474..3809
                     /locus_tag="PC472_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727407.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3841..5163
                     /locus_tag="PC472_002082"
     CDS             3841..5163
                     /locus_tag="PC472_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2727408.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5173..6243
                     /locus_tag="PC472_002083"
     CDS             5173..6243
                     /locus_tag="PC472_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2727409.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6248..7435
                     /locus_tag="PC472_002084"
     CDS             6248..7435
                     /locus_tag="PC472_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2727410.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7632..8504)
                     /locus_tag="PC472_002085"
     CDS             complement(7632..8504)
                     /locus_tag="PC472_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2727411.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8898..9089)
                     /locus_tag="PC472_002086"
     CDS             complement(8898..9089)
                     /locus_tag="PC472_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727412.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9309..10355
                     /gene="cobT"
                     /locus_tag="PC472_002087"
     CDS             9309..10355
                     /gene="cobT"
                     /locus_tag="PC472_002087"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2727413.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10387..10947
                     /gene="cobU"
                     /locus_tag="PC472_002088"
     CDS             10387..10947
                     /gene="cobU"
                     /locus_tag="PC472_002088"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2727414.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10957..11730
                     /gene="cobS"
                     /locus_tag="PC472_002089"
     CDS             10957..11730
                     /gene="cobS"
                     /locus_tag="PC472_002089"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2727415.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11750..12364
                     /locus_tag="PC472_002090"
     CDS             11750..12364
                     /locus_tag="PC472_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2727416.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12431..12610
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12740..14239
                     /locus_tag="PC472_002091"
     CDS             12740..14239
                     /locus_tag="PC472_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2727417.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14278..15654
                     /locus_tag="PC472_002092"
     CDS             14278..15654
                     /locus_tag="PC472_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2727418.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15655..16626
                     /locus_tag="PC472_002093"
     CDS             15655..16626
                     /locus_tag="PC472_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2727419.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16658..17728
                     /locus_tag="PC472_002094"
     CDS             16658..17728
                     /locus_tag="PC472_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2727420.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17730..18617
                     /locus_tag="PC472_002095"
     CDS             17730..18617
                     /locus_tag="PC472_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2727421.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18605..19237
                     /locus_tag="PC472_002096"
     CDS             18605..19237
                     /locus_tag="PC472_002096"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2727422.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19246..20472
                     /locus_tag="PC472_002097"
     CDS             19246..20472
                     /locus_tag="PC472_002097"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2727423.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20469..21077
                     /locus_tag="PC472_002098"
     CDS             20469..21077
                     /locus_tag="PC472_002098"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2727424.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            21067..21642
                     /locus_tag="PC472_002099"
     CDS             21067..21642
                     /locus_tag="PC472_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2727425.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21635..22387
                     /locus_tag="PC472_002100"
     CDS             21635..22387
                     /locus_tag="PC472_002100"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2727426.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22371..23492
                     /gene="cbiG"
                     /locus_tag="PC472_002101"
     CDS             22371..23492
                     /gene="cbiG"
                     /locus_tag="PC472_002101"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2727427.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23489..24214
                     /gene="cobJ"
                     /locus_tag="PC472_002102"
     CDS             23489..24214
                     /gene="cobJ"
                     /locus_tag="PC472_002102"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2727428.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKSGDTPVGVVKDVGREKEEKLVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24211..24963
                     /gene="cobK"
                     /locus_tag="PC472_002103"
     CDS             24211..24963
                     /gene="cobK"
                     /locus_tag="PC472_002103"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2727429.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24982..26460
                     /locus_tag="PC472_002104"
     CDS             24982..26460
                     /locus_tag="PC472_002104"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2727430.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26490..27395
                     /gene="hemC"
                     /locus_tag="PC472_002105"
     CDS             26490..27395
                     /gene="hemC"
                     /locus_tag="PC472_002105"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2727431.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27385..28884
                     /gene="cobA"
                     /locus_tag="PC472_002106"
     CDS             27385..28884
                     /gene="cobA"
                     /locus_tag="PC472_002106"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2727432.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28901..29866
                     /gene="hemB"
                     /locus_tag="PC472_002107"
     CDS             28901..29866
                     /gene="hemB"
                     /locus_tag="PC472_002107"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2727433.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30019..30597
                     /locus_tag="PC472_002108"
     CDS             30019..30597
                     /locus_tag="PC472_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2727434.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12413 a         3055 c         5664 g         9513 t
ORIGIN      
        1 atatactagg atataaaatt cttaaataaa aagaaaagat tgtttttata aaaaatattt
       61 tttataccac tttaaaaaac acaataataa cactgaggga ggtggggata tgagagaacg
      121 cattattctt gaaacattaa aaaatagtag tggttatcta aacatagatt attttgcaga
      181 taagttgggg gtatctactc gaactatcag aaatgaaata aaaaagatag aaactataaa
      241 agaatgtaat ggttttaaat tagaatataa gtctaaatta ggatatattc taaatattaa
      301 agaccaagat aaattcgagc attatctaag aaacttaccc tcagatttag ttgaaaatcc
      361 agaacaacgg ttggagtcta taatagtaga gcttttagta aatgaaggtt ataaaactat
      421 agaacagtta agtaaaaaat ttctagtaag ttcatcccaa ataaaaaatg atttaaaaaa
      481 gatagatgaa aagataaagg atacagaatt aaaactagag cgaaaggctc attatggaat
      541 taaaatagaa ggttctataa aagaaattca aagtatattg gtggatagtt attttagagg
      601 gaatagaaat ataacagagt acaggaataa gtttatagat aatttaaaat tagctaatat
      661 tagaagtaca ataaaaaatg tacttaatga acatgattta gaagcaaatt taacagaact
      721 tgaggaaata ttagcacaaa ttattatatt gtatataaga gttagtatga gagtagtggg
      781 aaatgctagc gatttaaaat tatatacaga agatttaatt atagataaat tattagataa
      841 aatttttaga gataaaaaat ataaccttaa ttatgatgaa aagtattatt taaaggagct
      901 tataaaacta aagactaaag ataagaaagc cactataaag aatattgata agaataaact
      961 tcaagatatt atgttcgaat tttttagaga gatagataaa aaatataact ctaatttttt
     1021 agaagataaa gagtttttta acttatttta cttacatatt gcatgtttaa tagaacgtat
     1081 aaagaagaac cacaagataa taaatccttt ttcagttaaa ataagtcaac agtatccaac
     1141 tgtttttaac ttatctattc aattttcaaa aatcatagaa aatgagtatc atataaaaat
     1201 aagtcaagat gaaatagggt ttatagctac tcacattgct gttccttttg aaaagagaga
     1261 agaagctaat tttaataaaa aatataaaat agcaataata tgctcttctg gtggaggtag
     1321 tgcatttttg ataaatttaa gacttaaaga aatttttcca aatgctgaga taagaaattt
     1381 ttcattacta gaagaaaaag ctgtaataga atttgcacca gatttgattt tttcaataac
     1441 aaatctttta tttgaaataa atgctccagt tatacttata aatgagattt tagacgaatt
     1501 ggattattta gatattaaag aaagtgttag atttgcagac catataggaa atatatcaag
     1561 cccaaaacaa tatatattag gtctatttga taagaatcac tttaggtgta taaaagataa
     1621 agctaagtat aaggatatcc ttgatagtat gtcaaaatgt atagttgacg agggagcttg
     1681 ttctccaacc tatcccaaag atgtatggga aagagaaagc tatttgagta ctatttatac
     1741 aaatggggtg gctatacctc atccaatcga gatgacagga aacaaaaata taatatcagt
     1801 agctttgata caaactgata ttatttatga aaatagggtg cctaaaatta ttttcatgat
     1861 ttcattaata aagggaaatt tagaacttca taagcaaata tctaaatatt taagtaagat
     1921 tatggcaaat aaggatatgg tagacatgct aaataagtca caatcttatg aagagtttat
     1981 gtataagtta aaaatctatc tgggaggtta gacatgaata tagagttttt taagaggttg
     2041 tcagaagcag atggtatagc atctaatgaa gaagaggtta gacatgtttt attggaagaa
     2101 ctaaaagctt atagtgataa aattatctgt gatgggctag ggagtatcat cttttctaaa
     2161 ataaaagatg agagtgcacc aaacgtaatg atttgtgctc atatggatga agttggattt
     2221 atggtgagaa gtatagacaa acttggtatg attcacttaa taacaatagg aggagtaaaa
     2281 cctcttgcac agtttgtaca aaaagttaga attacaacaa aagaagggaa gaaaatcccc
     2341 gctgtcataa atgctacata taataatggt aaggctgaaa atatatatgc agatattgga
     2401 gcatatacag aagaagatgt atataaccta gggattaatg ttggggatat ggtaacatat
     2461 acaacaagct ttgaggaatt tactttacca gaccgattag tgggaaaggc ttttgatgat
     2521 aggataggat gttttgtgat gggagaagtt ttaaaagaac ttaggaaaga aaatctaaat
     2581 tgtaatattc actttgcggc tacaagtagt gaagaggttg gaataagggg agcaaagaca
     2641 tcaacacagc taataaatcc agatatagta tttgtaatag atgtagcatg tgctaaaaat
     2701 gagtttgtga gagactatac aaatcagaaa caaattgata aaggtgtaat gctaatgcat
     2761 agagatagga ctctagtccc tagtagaaaa atgatagatt atgtaatgga aattgctgat
     2821 aaaaataata taccacttca acatgatatg tttgaatcag gtgggactga tggaggagaa
     2881 gcacatctcg taaatgaagg gaaaccttgt gtagtaactt gtgtaccagt tagatatggt
     2941 cattgtgcat tttcaatagt aagtaataaa gatttggaga acattataag attgtataca
     3001 cagttaattt taaattttga tgaagaacaa tataaacata taagaaattt tatctaaagg
     3061 agaataagaa aatgtcaaca gaagtacaaa taatggaact tatatcaact gcaggagaga
     3121 gtaaatcgaa agcttttgag gctttaaaaa aagtgaagtc aaaagatttt aaaggagcaa
     3181 aattgttaat agaggaggct cgtaaaatag atattgaagc tcacaggata caaacagaat
     3241 taataactca agatttaaat gaagatgttg aaagtccagc aatgagtctt ttaatggtac
     3301 atgctcaaga ccactatatg acttctcagc tagcaaaaga cttgatagag gctttgatat
     3361 attgtctcga taagtaattg taatgtagaa atagattatt tatgtaaaaa ttcttttaaa
     3421 agtttagatg cttttaagag ataataaaaa tacaaacttt aggaggaatt attatgaaaa
     3481 tattattatg ttgtgcaggt ggattttcta ctaatatgtt aatgcaaaat atgaaaaagg
     3541 tggtaaaaga gagtgctaaa ctcaatgaaa aagattttga gtttacagct ataccagcag
     3601 attcaataga aagtgaagtt gatgattggg atatagtact tataggtcct caaatagctc
     3661 acaaaataga cttcttaaaa ggaatattag agccaaaaaa taagcctttt gcagtaatag
     3721 ataaagatgt atatggaaaa atggatggag ctacagttat gaagttggca ttaataactt
     3781 ataagaaaca tcaattagct caaggataat aaaaataatg agggaaaagg gagattagat
     3841 atgttttcaa aatttgaagc atttatgaat agagtattta tgcctttagc tcataaggtt
     3901 gacaatcaac gtcaccttgg ttctataaag gctggtatgg ttgctatgac accattcaca
     3961 atattaggaa gtatatttgc aatacttcca gcacttccaa atatgcttgg agaaaataat
     4021 ccaataagca aattcatact taacaattca gcaatattgg atttaccagt aagtctttcc
     4081 ataggtttaa taggattata tgcttgtatg tgtattgctt ttaatctggg gaactactat
     4141 aaattatata taccaggttg tgttgcacta tcaacttttt cttttttatt cctagtagcc
     4201 tcatttacac cagatggagc tttagaaata ggaaatttag gagctaaagg gctatttaca
     4261 ggtatgatag cagggcttgg ttctgtggag ttatataatt tttgtaataa gaagaatatc
     4321 acaataaaaa tgccagaaaa tgtacctgat tttgtatcta ggtcttttga attaatacct
     4381 actactataa tagtttgtgg aacttttata gttgctcgtt ttatttcatt aaaagtattt
     4441 ggagaattac ctccacagat acttactcaa tttttatctc cattagtagg aagtatggat
     4501 aacccatggt cagttcttgc acttaacttt gctatatgta ctatattctt ttttggaata
     4561 cattcatcag tatttagtcc tataactaga cctataatga ttacatttat agctgaaaac
     4621 atagcagcta tgcaagctgg agaaacaata cctcatttct ttacagcagg agcttcaagt
     4681 gcattctttg gatttacagg atgtggaatt agtataggtt gtgtaattgc atgtatgtta
     4741 tcaaaaaatg cttcctataa gaagattgga cgtgtatcat tattccctgc attatttggg
     4801 ataaatgaac caatattatt tggagcaccg ataatattaa atccaataat gtttatacca
     4861 catgtatttg gtggagctat aataggtaca ctaccaatgt tttttatgca ctggggatgg
     4921 ttagcaaagc cagtatttga ccctccatat gtaggggtat tcttagaagg atttttaact
     4981 aatggagatt ggcattctat attagcaaat gcattgcaat tagtattagc agttttatta
     5041 tattggccat tctttaaaat aatggaacgt caacaaaata ataaaaaaca aaaagaagaa
     5101 actaagaata tattctctaa agaagaagaa gacttattgg atgatttagg attggatttc
     5161 taatattttg ttatgaataa tacaatggag agattagtat ccttaatcta taaaagaggt
     5221 gttgacgctc ttttgattaa agcaaaaaat actaaaagat atataggagc tttatcaggt
     5281 agtggtgtat atgtgcttgt tactaaggat aaaaattatc aaattctaga tggcagatat
     5341 attgatgaag cagataaaaa aacgactggt ttcataaaaa ggattgtatc tcaaggttct
     5401 tatataccaa ctataattga gttattaaaa gaattaaata taaaaaagtt agcaatagaa
     5461 aaccaagcta tgagtataca agagtatctt tcactaaaaa atgaaggttt tgaaatcaat
     5521 ttaataacaa atgaattatg gaagctaagg gctataaaat ctaaagaaga aattgaactt
     5581 attaaaaagg cttgtgaaat tacagatgaa gtatttgatg aggttatttc tgaaattaaa
     5641 aaagatatga ctgagctaga agtatcagct ctaattcagt atcatgcatt aaaaaaaggt
     5701 gcaagtggaa tgtcttttga aacaatagtg gtatcaggag aaagaggtgc aatgccacat
     5761 ggaagaccta caaacaaaaa attgaaagtg aatgaagcta taacaataga ttttggtgtt
     5821 gtttatcaag ggtatcaatc agacatgact cgtacaatat ctataggtaa acctcctaaa
     5881 attattaaag agatatatga tgtagtattg gaagcacaat tatctgcaat agagtcaata
     5941 aaggaaggaa ctagggcaag tgatgttgac aaagtggcta gaaaaattat agataaacat
     6001 ggatttggcg aatattttaa tcatggactg ggtcatggta taggtcttgg agatggtgaa
     6061 gttcccactt taaatccaaa tagtgaagat atattagtgg aaggaatggt aatgtcttgt
     6121 gagccaggta tttatattcc aaatgtaggg ggagttagaa tagaagatga tattgttata
     6181 atagatggaa aaggtgttcc actcaataag acatccaaag agtttataat attaggagag
     6241 taaaattatg aaatataatt ttaataaagt attaaatcgt aaagaaggaa attgtagaaa
     6301 atggagcaac catgtgatta aagaaaaatt tggcttaaat gaagatgcaa ttcctatgga
     6361 tttagctgat atcgattttg agtgtgctcc tgctataaaa gaatctatct taaaaagagc
     6421 atcagtagga gattatagtt atacttttat aggagatgac ttctatgatg ctattataaa
     6481 ttggaataaa agaagatttg atgtatatat agaaaaggat tggataaaat taacttttgg
     6541 tactgtttca acccttcatt atatagtcca agcatatact aaagaaggag agggtgtatt
     6601 gataaataca cctgcttatg acccttttgc agaggctgta gtaaataata atagaaagtt
     6661 gtgttgtagc tcattaaaat tagataacaa tagatattat ttagatttag aggatatgga
     6721 aaatcaaatg aaatacgaga atataagatt gcttatattc tgtagtccac aaaatccatc
     6781 aggtcgtata tggacaaaag aagaactata tcaagtgtca gagttatgtt taaagtataa
     6841 tgtaattttg gtaagtgatg aaattcatag agatataata tttaaagatt atagatttgt
     6901 atctttgtgg aatgctcatc cagaaattta taaaaatagt ataatctgtg tatctcctaa
     6961 taaaggcttc aacttaggtg gattaaaaac atcatatgta ttaataagag atgtaaaaat
     7021 tagggagact ttattagaaa ggctaaaaag taattcaata acttctccaa atgtatttgc
     7081 aataccagca atagttgcag catataatga aagcgaagaa tggctagatg ctatgactag
     7141 ttatgtagag gagaattttg aaatagttta tgatttcttt gaaactaata taccaaaagc
     7201 taaagtgatg aagtcagact cttctttttt agcctggata gatgtaagag aagtatttag
     7261 aaatgaagaa gaatctaaag aattttttag acatgcaaac ttaactatgg tagttggaag
     7321 ttattttgtg aaagatggag atggatttat tcgtattaat gtggggtgtc ctagagaaac
     7381 attaaatgag gctctaaata gaatcaaaaa aacgtatata agtatgtatt gttaaatcat
     7441 aaaaaaagta tctatggttt atataaaaac acattattta gttatcaaat attactggaa
     7501 ttttgtattc cagtaatatt ttttgattaa ggaatattat tgaggaatat taaataatat
     7561 tatttcatac ccttatatta acttatataa aaatatttta ttattttggg aacgggttag
     7621 ctaaccttta tctagtgtat atttatattg taagaagcag taaaaacttt tcctttacta
     7681 agtttattaa taaacttctt atctttataa attttgttag aatttatact atcagcaaga
     7741 ccataccaag gttctataca taaaaaagga gcagtagaat tagtttcact ataataagga
     7801 gtccaaatac caactagagg gaaatcttcc atagatatag ttacatattt actaccatca
     7861 ttattacata tagacacttc atctatgttg gtgtatatca aagcatcatt cttgaatact
     7921 tctggattta actgtaagtt ttttaatttt ccaataggta ttatatcatc tgtaaaagaa
     7981 ccatttaaat tgattttttc tacatcattt ttacttttaa actcaaggta gtgtttagaa
     8041 aaatcatttt gttcgtaaaa aggaaggttg aatgcaggat gtccacctat agaaaagaat
     8101 atatctttag aatcagtatt ttttacaatc cattctacct caatactatt atcatttaat
     8161 gtatagttta tgattagctc aaaagaatat ggatacttct ttaaagtaga ttcattatct
     8221 gttaatttat aagttacaaa tgtatctcct ttatctacaa tctcaaagtc catatctcta
     8281 gcaaacccat gttgagtcat gctatataaa ttttcttcta ttattgtttc attatcaaaa
     8341 agtttaccta ctatagggaa caatataggt gattgtctac cccaatattt tttattacca
     8401 tcccaaagaa tgtttttgtc gtatttttta gaaaatattt tagttagttc agcaccaaaa
     8461 ctgcttgatt cgataattaa attttcattt tgtaatatat tcatttaaaa tctcctttat
     8521 tatattttat gagtatatta tactataaag tataaagatt attaaatttt atttgatatt
     8581 atatttgaaa ggtagatttt agtatatttt gaaaattttg ttttgaaaat ttgggattat
     8641 ttaagaaatt ttgtattata aatagaagga aatagataaa ttgtatagaa taaaatatat
     8701 taagataagt taataatgtt aaatgggaag tgagtttatg gaaaaaccaa atacaaaagt
     8761 tgttaacttt caagagtata aagacagaaa aaaccaaaga gaagtaaata gagacgaact
     8821 tttaaagctt ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac tccctatttt
     8881 aatgggtttc ataataatta agacattcta agtccatcta tataatcttt gaattgtgag
     8941 aaatctgatt cactacttaa ttctatggtt tctgaacctt ccgtataaaa attcttaaga
     9001 tagatagtat aattattatc aacacaatca tgataaatac atctatatcc atcttcatac
     9061 aaacttttta acttagaaaa atcatccaca atatcatctc cttaaagttc ttttaaatat
     9121 atttttacaa aaagcatcta attgtatgca aattgacaat gtaaaataaa ggaaatgaac
     9181 ataaaatgta gaataatttt tatatgtatg taaaaataaa aagaaaaagt tgaaattttt
     9241 agaaaataag agtatttttt atctaaaaag agagtttaaa aaataaaaaa atgttgggag
     9301 gataaaaaat gagtttatta gaaagtatat caaaaaacat atattcccta gataactctt
     9361 ctattgaaaa aacaaagcag agattggata ggcttataca tccaactgga agcttaggaa
     9421 agatagaaga tatttgcatg caattagcag gaatatttgg caatgaaaat tttgacacaa
     9481 gtaaaaaggt tataattgct tttgcgggag accatggtgt atatgaagaa ggcgtagcac
     9541 ctgaccctca aaatataaca aaattacagt ttccaaattt ttcgaaaggt ctatgtggag
     9601 ttggagtaat aagtaaattt gtaggggcag atgttgtagc tgtagatgta ggaataaact
     9661 gtgatgaaaa actggatggg gtattagact acaagataag aaaagggaca tcaaatatgg
     9721 caaaaggtcc agctatgagt aaacaagaag caattagatg tttagagata ggtattgaaa
     9781 tagcagagca gtgtatagaa aaagattata aggttattgg tattggggag atgggtatag
     9841 caaatactac tccaagtaca gcaataatat ctgtaatttc agggtgtgac ccgttagaag
     9901 ttactggtat aggtgcaggt cttaagaaag aaagactaaa gcataaggct gaagtaatta
     9961 gaaaggctat tgaaattaat aatccaaatc caactgatgg agtagatatt ttatctaaag
    10021 taggaggatt tgaaataggc tcaatggcag gtgttatatt aggttgtagt gctaatagga
    10081 ttccagtagt aattgatggg tttatttctt atgcagcagc attaattgca tataagataa
    10141 atccaaaaac tagagaatat atgatagcat ctcatttatc agcagaatca ggaactaaaa
    10201 gagcattaga catattaaaa ttagaccctc tattaaatat ggatatgaga ttaggtgagg
    10261 gaagtggagc tgctttagca tttgatataa tagaagcttc aaactatact tacaaaaata
    10321 tggcaacttt tgatgaaatt gacatgggaa gataaatata aaaatttaat atgtaggagt
    10381 gttttgatga gtaaaattat tctagtaaca ggaggagcta ggtcagggaa gagtaacttt
    10441 gcagaaaaac tatgttttga cagaaataat aatacagctt atatagcaac ctctattcca
    10501 tttgatgatg aaatgaaaga tagagtaaaa aaacatcaag agaatagacc tagaaactgg
    10561 aatacttatg aaatatatga agatatatat tccataatta aggagatata taaagaacat
    10621 gaaactgtta tactagattg tgtaacatta ttagtaaata atctaatgtt tgaacacaat
    10681 ctaaatatag aagaatctag tcaagaagaa ataaacaaat tagaacaata cataaaagaa
    10741 caagttgtca aacttattga agaaatagaa aaaacaaatc tatactttgt atttgtaacg
    10801 aatgagttag gtatgggaat tgttcctggg aataaactta gtagaattta tgctgatatt
    10861 gtaggtcgta taaatcaata tatagcttcg aaaagtaatg aagtttattt tgtagttagt
    10921 ggcatagcta tgaagataaa ggagtagtat gcaattatga aaagatttat tttaatacta
    10981 cagtttttga caaggatacc tataaaatta aatgtaggat ttgatgatga attttataag
    11041 tcaatagtat attttccact tgtaggtttt gtcataggta tattatccta tttaatagga
    11101 tggatttcta tgcttttatt tgaaccattt atagcttcta taataataac tctagctggt
    11161 gtcttaatta ctggaggact tcatatagat ggattaggag atacatttga tgcaatatat
    11221 agttataggg ataaagaaaa gatgcttgaa ataatgaaag attcaagatt aggtacaaat
    11281 tcacttctag caattatgtt tgtactttta ttaaaggttg gattcgttta cgatataata
    11341 agtaataatt cattgtgggt tataattttt atgccaatga ttgcaaggtt aggagtaatg
    11401 ttattgacat ataaaactgt gacaccaaga gaaaaaggaa tgggaaattt atttatagga
    11461 aaattaacta caagtatgtt aataactgct ataatataca cattattgat agttgctctt
    11521 attactaaat ttatattttt attacctaat atagtgttga ttaaagtatt aggttcgatt
    11581 atagtggttt ttgtatttat aatattattc aaaaaacata tttataaaaa aattgacgga
    11641 gtaacaggag atatattagg ctgtggaata gaactttcag agcttgtata tttaatttac
    11701 atatatttgt taatttttat gtttttttaa agtaaaaagt ggtgataata tgataagatt
    11761 aatattaata agacatgcac tgacaaatga taataaaaaa ggaagattat caggccatat
    11821 aaatagttgt attagtgaag agggcaaact acaaataaat aaaattacca gatatctgag
    11881 taatgaaaac atagataagg tttatacaac accatctaca cgtactaaag atacagtaga
    11941 aaaaatatca aaattgaaat tattagaaat agaagaaaaa gaagctttaa gagaaattag
    12001 ttttggagac tttgaaggaa gaacttttga agaaattaaa atcaaatatc caaatgagtt
    12061 tgaaaaaatg ataaaagaag gtaacaacta tagatatcca aatggagaaa gtttgataga
    12121 ctcttataaa agagtggcga aagaaattga taatataatt ttagaaaatg atagtaattt
    12181 agatacaaag acaatactta tatgttctca tgcaggaaca ataagaaata taataacaca
    12241 tttgatttct ggtagttata agtatcactg gaactttaaa attgataatg catctattac
    12301 agttttagaa attgatggtg gatttgctgt aatagataaa atgaatttta cagacttcat
    12361 ataatttaat caaaaccaaa cattacaatc tgtgtttgta atatatctgt aaaattagca
    12421 atgaaaatag aatattagat taaaattagg ttcttaatta gatgttaaaa gggaaaaagg
    12481 ttagatgcct ttgcagcccc cgctactgtg aaaccaacga aactataaat accactgtca
    12541 gtttgatggg aaggttatag aagtaggatg aagttaagcc aggatacctg cctaatttaa
    12601 tttaaaacaa gaggtcttcg gggtgaaaga tttttaatta atatatataa aagtcctatc
    12661 tttagaagta ttaggacttt ttttaatttg ttttttagta aattttaaca aggagggatg
    12721 gaaagtgggg aaaaaaataa tgctacaagg aacagcttca aatgtaggta aaagtgtctt
    12781 agtagcaggt ttgtgtagaa tatttaagca agatggttat actgttgctc cttttaagtc
    12841 acaaaatatg gctttaaatt cttttataac taaagaagga ttagaaatgg gaagagcaca
    12901 agtatttcaa gcagaggctg cgaaaataga gccaatagct gatatgaatc cagttttatt
    12961 aaaaccttct ggaaataata aaagtcaagt tattgtaaga ggtaaagttg taggagaaat
    13021 gccttcttct aagtatcatg attataaatt ggagttagta gatgtgttga aggaaacatt
    13081 taatagctta aattcaaggt atgatattgt agttatggaa ggggcaggaa gtaatgcaga
    13141 gataaactta aaagatagag atatctctaa tatgggtatg gcagaaatag ctgatgcacc
    13201 tgtaataata gtcggagata ttgatagagg aggcgtattt gcctcattag caggaacaat
    13261 gcttttatta aatgaagatg agaaaagaag agtaaaaggt gtaataatca ataaattcag
    13321 aggaaaaaaa gagcttttac aagatggtgt aaagatgcta gaagacatca taaaagtacc
    13381 tgttatagga gtagtacctt atacagatat aaaaatagaa gatgaagaca gtgtaactac
    13441 gagatttaag aaaaaaatga ataaaagtga tatacacata gaaataatta gaactccaca
    13501 tatgtcaaat tttacagatt ttaatatatt tgagacacaa gaagatgtaa gtattagata
    13561 tgtagactat ggagaatctt taggaaatcc agatatatta ataatacctg gaactaaaag
    13621 tactatagac gatttaacat atataagaaa aagtggtctt gaatcgcaaa tcaaaaattt
    13681 acatagtcaa ggtaaattga tatttgggat atgtggtgga taccaaatgt taggtaaaaa
    13741 attaaaagac ccttatcatg ttgaaagtga aattgaagaa gttgatggaa taggtctttt
    13801 agatacagaa acaacgtttg agaaagaaaa gactacaaca caagtagaag ctactattgt
    13861 tgaatgtatc agtgaataca tgaataacct taagggaaaa aaagtaaaag gttatgaaat
    13921 tcatatggga attacaaagc gaggtaatga tgtaagaagt ttagatttaa taactaaaaa
    13981 acttgataaa gaagtgaatt atactgaggg aagtgtaaat aaagagggga atgtaattgg
    14041 gacttatctt catggaatat ttgatgaaat aagctttaca agagcaattt taaacaatat
    14101 aagaaagaaa aaaggtttag gagaattaga aagcaaagta aactctttta atgaatttaa
    14161 ggataaagag tatgataagt tggctgattt tttaagagaa catttagata tagagaaaat
    14221 atatgagatt atgaaataat gatgactttt atttataatt taggtaggag gtgtattatg
    14281 aaaaaaattt taattgcggg tactaatagt ggagtaggta aaacaacaat atcattagga
    14341 ataatgcaag ctttggttaa gagaaatatg aaagtacagc catataaagt agggccagac
    14401 tatatagacc cttcatatca cacttttata actggaagac attctagaaa tttagattct
    14461 tatatgttag atgatgaaaa aataaagtat atagtaaata agtcctcaaa agatgcagat
    14521 atttcagtaa tagaaggtgt tatgggctta tatgatggct ttggaataga tttagataat
    14581 tgtacaagtt cacatacttc aaaggtatta aagtcacctg ttatattagt tataaatggt
    14641 aaatctatgg cagcatctag tgctgctatg gtacttggat ataaggaact agataaagat
    14701 gtaaatataa agggtgttat agtaaataat gtaaagacta atagccatta tcaaataatt
    14761 aagagttcta tagaaaaata ttgtaatgtt gaagtattgg ggtattttcc tccaaataac
    14821 aaattctctt tggaatctcg acatctagga ttagttccta gtgtagaaat gaaatcacta
    14881 agggaaaaat tttatacctt agcagatgaa attgaacact atatagatat tgacagaata
    14941 attgaaatat cagaaagcga tgaatttgag agtgattttg attttcaaac tttaatagaa
    15001 aataataaaa taaaagaaca tgttaataat aaatcaatag ctatagcata tgataaagca
    15061 ttcaattttt attatagaga aaatatagag cttttagagg aacttggttt agaaataaac
    15121 tattttagcc ctcttgaaga tgtatcagtg ccaaatgcag attatattta tattggagga
    15181 ggttttccag aaatatttgg aaaagaatta gaagcaaatg agtcaatgag aacttccata
    15241 ttagaagcac ataacaataa tatacctatt tatgcagaat gtggaggact gatgtaccta
    15301 ggtgagaaac tattgaatca agaagaacaa gaatttaata tggttggaat atttagtgga
    15361 attagtaaga tgacatcttc tttaaagaga tttgggtact gtattggtgt agccaaggaa
    15421 aatactcttt tatctaaaaa aggggaatct attaaagggc atgagtttca tcattcaata
    15481 tttgaaagtg atgaaaaatg tgtatattca atgagaaaag aaagagatgg aaatatagta
    15541 gaagagtggg atggtggtta tagtaaagga aacactttgg ctacatatct tcatactcat
    15601 ttttataata acttaaattg tatagaaaat tttttgaaaa aaggaaatga ataaatgagt
    15661 attttatcta tttatatagg atatgtgaca gacttaattg taggagaccc atattctttt
    15721 cctcatccag tgagatttat tggtaaactt ataaatttta ctcaaggtat aataagaaag
    15781 gtctctaaaa atgataggca gttaaaattg gggggattta tattatggtt tataaccgtt
    15841 ggaataacat atttaacaac ttatgctata gtgaagttat ttagttttaa tatattattg
    15901 agtattgtag tgaatagttt tataatttat acaacactag ccacaaaatg tttaaaagac
    15961 gaagcattaa aaatttataa tgtattaaaa actgaagata tagaaaaatc taggattcag
    16021 ctatcttata tagtaggtag ggatactact aatttaagtg aacctgaaat aataagagca
    16081 actgtagaaa ctgttgcaga aaatactgta gatggtataa ttgcgcctat gttttatgca
    16141 tttattggag gagcaccact tgctatggcc tataaagcta tcaatacatt ggactcaaca
    16201 gttggatata aaaataaaaa gtataaagat attggttttg catctgctaa gattgatgat
    16261 atagccaatt atataccagc tagaatatct gtcattctta tgacaattgg aagtttcttt
    16321 ttaaattata actataggaa ctgttttaaa atctctatta gagatagaaa gaatcataaa
    16381 agtccaaatt gtgctttttc agaaggtgca gtatctggtg ctctaggaat acaactagga
    16441 ggtaccaacg tttattttgg ggaaaaagta tataagccaa caatagggga taagcttagg
    16501 gagatagata aagaagatat aattaaaact aataaaatta tgtatgcatc ttcatttgta
    16561 tctatgttgg tatttacact aatatatgtg ttatgtagga tgattttaat taacttaatt
    16621 atataaatta ttgtttgtta acttaagggg gacatagatg aaggatttag ggcatggtgc
    16681 caatgtagat gaaatggcaa gattatatgg gaaaaaccca aaagaaataa ttgattttag
    16741 ttcaaacata aatccaaatg tattaccaaa tttagagaga tacattttaa aaggattaga
    16801 agagtgtagg aattatccag atataaatta tacaaacttg agagaaaata tatcaaaata
    16861 tatagatata aatccagact ttataatacc tggcaatggt gcaacagaag tgatttattt
    16921 gcttatgaag agtattaaaa aaaaattggc tataataaat ccgacttttt cagagtatag
    16981 aagaagtgct gagttaaata atttagatat tatagattta gagttagatt tagaaaataa
    17041 ctttaaactt aatatagata taataaaaga aaatataaaa agatttgata gtttatttat
    17101 atgtaatcct aataatccga gtggtaatgt ccaagattta aaggaacttg ttcatttact
    17161 agataaacat aataaggtat taataattga tgaaactttc atggaatttg ttgaagatga
    17221 aagtaaatat agtttggtta aatatataga atcaaataaa aatatattta taattaaagc
    17281 tgtgactaag ttttttggta tgcctggttt aaggctagga tatggtctta caagcaacac
    17341 tgaaattatg aacaaaattt atgaacacaa agagccatgg actatcaatt cttttgcaga
    17401 tatactatcc aattttattt ttgaagataa agagtatatt aaaaatagta aagaatatta
    17461 catagaagaa agaaaatata tgttacaaga gttgagaaac ataagaaata taaaggtcta
    17521 tgatacagat gctaatttta tcttgataag aatatataaa aaaacaacta aagaattgaa
    17581 aaaagatttg tttaagcaag gaaacatatt agttagagat gcatctaatt ttataggatt
    17641 agatgatagt tttataagag tggctataaa atctcatgaa gataacaaaa ttcttataga
    17701 aaatatgaaa aatttattgg gtgattaaaa tgaaatctta tggaatatgc ccagcatctt
    17761 gtggagaatt tgtacaaggt ataattgatg atgaagaata tttatgttca tatgcaatag
    17821 atatgtattc taaagtgtat attgaagaaa aattagtaga tataaattta gggagatata
    17881 agtcaaggtt agcaattgaa aaggtatttg aaaaatttaa tttacctaaa aaatatacta
    17941 aaaatatttc tttaaatata aatagtaaaa tacctgttgg aaaaggaatg gcaagttcaa
    18001 ctgctgatat aggagctact ataaaggcaa cattatcatt gatagataaa gatttaagta
    18061 gtgaggagat ttctaaatta gcagcagaaa tagaaccaac agattctata tttatagata
    18121 aaaatagtat atttaatcct ctaaatggaa ctgttataaa atacctagga aatctgacca
    18181 atgcgaaagt tgtaatactt gaaccaaaca aagtactaga tactatgaaa attagattga
    18241 ggcaagatta taataaatta aaagtagaaa ataaagaagt aataaaaaaa tcatttgctc
    18301 ttttagaaga aggattgaaa aaaaataatt tatctctagt tggagaggct tgtactttga
    18361 gtagcttagc aaatgagaat atagaaaaaa aagagtattt aaatgaaata ataaaaatat
    18421 caaaaaaata tggagcatat ggagtaaata ttgctcacag tggaactgta gtaggtattt
    18481 taattgataa gtctatgaat gataaaaaga tgatagatgc cttatgtgaa tctaacataa
    18541 attctgtcta taataaaatt tatacacaaa acataattaa tggaggaatt aagggggaaa
    18601 tagaatggaa tacataaaga atcctatgaa aatagaagaa aaaagttttg aattgataca
    18661 agaaataata gacgaaatta gaccagatta taaatttaag aataatatcg aagaaaaaat
    18721 aataaaaaga gctatacata ctactgctga ttttgattac ttagatatat taaaaatatc
    18781 agaagaagca gttgatagta taatagatgc attaaaaaat aatgcaagta tttatactga
    18841 tacaaatatg gctcttagtg gtataaataa aagaaagtta gaatcattag gatgtaagta
    18901 taaatgttta gtggcagatg atgaaactgt aaaattagct aaagaaaaag gaattactcg
    18961 ttctatggca gcagtagaaa ttgcagctaa agaagaagga agaaaaatat tcgttttagg
    19021 taatgctcca acagctttat ataaagttat ggagatgaaa tcagaaggta gattagattt
    19081 agatgcagtt ataggagttc ctgtaggttt tgtaggagca caagaatcaa aagatgaagt
    19141 tgaaaaaaca gatataccat atataatttc taaaggtaga aaaggtggca gtaatttagc
    19201 agcagcaata gtaaatgcta tattatatac tatgtaggta cctatatgga agaatatgta
    19261 tatatagatg gaaaaaaata tagaagagga tatactacag gttcatgtgc aacaggtgca
    19321 tctaaggctg ctgtatatat gttgattaca aaaaatagaa tcaatactat aaatatagat
    19381 actcctaagg ggataccatt actactaaaa gttgataata taaatatttc tgatacattt
    19441 gtggaatgtt ccataaaaaa agatggtgga gatgatatag atgcaactca taccatggat
    19501 atttatgcaa gagctgaaat tgtagctaaa aatgataaga acaaagggta tcttactcta
    19561 aaggatatag atagtttaag caccaatagt gagtgtaaaa gtgagctcta taagtttatt
    19621 agggtttatg gcggaactgg tattggtgtg gttacgaaaa aagggcttag tgtggatgtt
    19681 ggtaagcctg ctataaatcc gactccactt aaaatgataa atcatgaaat aagaaagctt
    19741 ataggagata attttgaatc tatacttgga aatgataaag ttttaaaaat aacaatcttt
    19801 gctccacaag gagaaactgt agcaaaaaaa acatttaatc caagattagg tatagttgga
    19861 ggaatatcta taataggtac aactgggatt gttgaaccta tgagtgatga gggttggaag
    19921 aagtctctat ctatagaact tcagatgaaa aaagaacaag gcttagataa aataatattg
    19981 gttccaggaa atcatggaga acagtttata agagaaaaat taaacttaga tattaagtat
    20041 gtagttaggg taagtaattt tataggctat atgattaaag aagctcaaag aataggatat
    20101 aagaaaatct taatggcagg tcatataggt aaatttataa aagtatctgc tggtattttt
    20161 aatactcata gtaaggtagc agatgctaga agtgagatat tagtggcaaa cttagcttta
    20221 atgggagcta gatacgaatt tttaaataaa ataaatcaat gcgtaactac tgaagaagct
    20281 gtagagctaa taaataatag tgaatataga gaagtatata atatattaag taataaatgt
    20341 agggagagag taaagcaata cttgaatgaa gattcagatg atattgatgt ggaagtaatc
    20401 atattttcaa tggataagtc tttattaggt aaatcggata atacagatga tttagtggag
    20461 gtctttatat gataaacata ataggtctag gacctggcaa cttagactat ataacaaaaa
    20521 aaggtgaaaa tttaatttcc acttctgatg tattaattgg tggtaaaaga aatttagagt
    20581 ctataaaaaa ttttgaagga gaaaaaatag ttctggattc taatttaaga gaaattatag
    20641 agtacataaa taacaataaa gaaaagcaaa tatcaataat agcatctggg gacccattaa
    20701 tatatgggat aggtagatat ctatctaaaa atattgataa taagatgtta aacatggttt
    20761 ctgggattag ttcattacag tacatatttt caaaaatata tgtagatatg aatgatgtat
    20821 atattacaag tagtcatggc aaagtaccag attttgatta catattatca cataaaaaag
    20881 tatgtatggt tacagattca aagataggac caaaacaaat aagtagagaa ataatagata
    20941 gaaatctaaa taaaattatt gttgtaggag aaaatctgtc atatgacaat gaaaaaataa
    21001 caatagcaaa accagaagag ataataagaa tagacaattt tgatatgaat gttgtggtga
    21061 ttttagatga ggaatagtga gtttataaca ggtaaagttc caattactaa ggaagaggtt
    21121 agagcaatat caataagtaa gttaaattta gtaaatgcta aaaacctcat agacataggg
    21181 gctggtacag gaagtgtaag tgttgaagct gcatataatt atcctaattt aaaggttata
    21241 tctatagaga aaaatgatga tgctatagag cttatagaga aaaataaaaa aaaattcaac
    21301 ttaaaaaata tagaagtgat aaaaggatat gctccaatta atctagggtt aaatgttaaa
    21361 gttgagtcta tttttcttgg gggaacagga aataatctgg aagaaataat agaatggtct
    21421 aaaaaaaatc tcgttacagg aggaagactt gtagctaatt ttataataat agatacattt
    21481 aatcaaactt taaaactgct taaaaagcat ggatttaaag aaatagatgt atgtgttttg
    21541 aatgtttcta aactagagaa actaggaaaa ggtgaatatt ttaaaccact aaatcctata
    21601 tatataatat cttgtgaaaa aggggaaaat gatgatgaat aaagtacatt ttgtaggagc
    21661 aggacctgga gataaagagc tgataacatt aaaaggatac aaattattaa gtaatgcaga
    21721 tgtagtgata tatgcaggtt cacttgttaa tcctgaactt ttggaatact gtaaagaaga
    21781 ttgccaaata cataatagtg cacatatgga tttacaagag ataatagatg ttatgagaga
    21841 aggtatagaa aataataaat ctgttgtaag actgcaaaca ggtgattttt ctatatatgg
    21901 ttcaattaga gaacaagtgg aagatttaaa taaattgaat atagattatg actgtactcc
    21961 aggagtaagt tcttttttag gagcagcatc atctttaggg gttgaatata cagttccaga
    22021 aatttctcaa agtgtaatta taacaagaat ggaaggcaga actccggttc cagagaaaga
    22081 atcaatacaa tcatatgcta agcatcagac atctatggta atatttttat ctgttcaaga
    22141 aattgagaaa gttgtgtcta aattactgga aggtggatat ccaaaagata ctcctatagc
    22201 agttatatac aaagcaactt gggcagatga aaaaatagtt aaaggaactt taagtgatat
    22261 agctgtaaaa gttaaagaaa acaacataaa taagactgct ttaataatgg ttgggagatt
    22321 tttaggagaa gagtataata attcaaagtt atatgataag gatttcaaac atgaatatag
    22381 agggtaatat tgctataatc tgtattacag aaaatggaaa aaaattagct ctaaagataa
    22441 attctctagt aaataactgt gttatatatc aagtaaaaaa taaaaaaagt aatttagttg
    22501 ttgaagaaac atgtgtacat acagttcaaa agaagttaag tgattttgta ggagaaatat
    22561 ttagcaaatt tgattacata gtatttataa tggctacagg tatagttgta cgaagtatag
    22621 caccatacat aataagtaag tttagtgacc ctgcaatttt ggtaatggat gaaaaaggca
    22681 aaaacgtaat aagtttattg agtggtcata ttggaggggc aaacagcttg acacagtaca
    22741 taagtagtct tattgatgct aatccagtaa taacaacagc tactgatata aatcaaaaag
    22801 cttccttaga tatgatagca aaaaaactaa atgcacatat tgataatttt agagaaagtg
    22861 taaaagaagt aaattctatg ctagtgaata ataaatgtgt tggattatat attgatgaag
    22921 gatatcaagt tgatactagg ggatttaaac ttatagaaag taaggatgag ttaagagata
    22981 gtttttcaaa cacatatata gaaaaattag ttgtagtaag caataaaata aattgtattg
    23041 aagatattga gaatttaaac ctagataata aatttgaaca ttgctatagt accaaaaaaa
    23101 taataaaggt aattccaaga gatatagtga taggaattgg ctgtagaaga aacacagata
    23161 gtgagctgat gtataattca cttgaagagt ttcttaataa acaaaatata gatattagag
    23221 caataaacac agttggaagt atagaactga aaaaagacga aaaagcaata attgaattat
    23281 caaaaatatt gaaggtgcca tttaaaacat tttcagtaga tgaaatatct aaggttgata
    23341 atctttttga taaatctgat tttgttaaaa aaaatgtggg cgtgtattca gtagcagaac
    23401 ctgtagcata tcttttgagc aaaggtaatt tgttagttga aaaacataaa tataaaggaa
    23461 taactttttc agtggggaga ataaaaatat gatatacgtt gtaggaattg gaccaggaag
    23521 taaagataca atgactttag aagcaataaa agcaatagaa gattctgagg ctattgttgg
    23581 atataagact tatataaaac taatagaaga atttatacaa gataaagaaa taatccaaaa
    23641 tggtatgagg caagaagttg atagatgtaa acaagcagta gaggaagcaa aaaaaggaaa
    23701 aaaagtagca gttgtaagta gtggtgatgc tggaatatat gggatggcag gtcttatttt
    23761 agaattaata tctaaagaag atgaagatat aaaagtaaag gtaattccag gtgttactgc
    23821 aagtattggt gcagcagcta tacttggagc tcctattatg catgatttct gtcatataag
    23881 tttgagtgat ttaatgactc cgtgggaagt aattgaaaaa agattgagat tggctgctga
    23941 agccgatttt gtaatttgtt tgtataatcc aagaagtaag ggaagaagtg aacatctaag
    24001 caaagcattt aaaataatgg gtgagtttaa aagtggagat acacctgttg gagtggtaaa
    24061 agatgtaggt agagaaaaag aagaaaagct tgtatgtact tttgaaaata tggactttga
    24121 aaaagtcgat atgactacaa tggttataat aggaaataag tctacctata ttaatggtga
    24181 attgatgata actccaagag gttatacagt atgattttaa ttttaggagg aacttcagat
    24241 agtttatcta tatgtgataa gataaatgaa ctgaaaaatc aaccatatat cctttcagtt
    24301 actacagatt atggaaggga acttgcacaa caacatgcag agaatgtaat tttaggtaag
    24361 cttggaaaag aagatatgct aaaatttata aaagataata atatagtaaa aataatagat
    24421 gccactcatc cttatgcagt tgaagtatcc aaaactgcta ttacttgtgc aaaactattg
    24481 aatatagatt atataaggtt tgagagaaaa tctttaattg aagaaataga ttatgataat
    24541 aaatttatag tagatacaat tgaagaagcc tgtgaaatag caaataaaat agggacaaat
    24601 atattcatag gaacaggaag taaaaatttg aacagattta tagaggaaat aagtgataaa
    24661 aatttagttg ttagagttct tccaacgagt gaagtcatat taagttgtga aaaattaggt
    24721 ttaagtgcag ataatatagt agctatgaaa ggtccattta gccagtatat aaatgaagaa
    24781 acatataaac attacaatat agatttagtc ataacaaaag aaagtggaat agctggtggg
    24841 ttcttagaga aggtaaatgc atgtaaaact ttcaatatac cagtaataat tataaaaaga
    24901 gaatcgatta actacgaaaa tgtatttaat gacataaata aaattaaaga catacttgtg
    24961 taatttttag gaggagaata tatgaagaaa gccttattag tagtgagttt tggtacaagc
    25021 tatcatgaaa cgagagaaaa gacaatagat atatgtgaag ataaaataaa aaattcgtta
    25081 aaaagccatg atttttttag agcttataca tcaaatatga taataaataa aataaaaaaa
    25141 agggatggaa tagaaataga taatccaata caggctttag ataaaatata tgaacaagga
    25201 tatgatgaag taataatcca aacacttcat attatatgtg gcgaagaatt taacaaatta
    25261 aaagaacaag tagatggata ttcttctaaa ttcaaaaaga ttgtattagg aagacctttg
    25321 cttacacata ttgaagatta tcaggaagct gtagaggcaa ttaagcatca aattcctcat
    25381 atggaaagta atgaagcagt agtatttatg ggacatggaa cacttcatga atcacattca
    25441 gcgtatccag cattggaata catgcttagg gataatggaa taaatgctta tgttggtaca
    25501 gttgaagggt atccagaaat agaacatgta ataagaagat taaaagaagg aaatataaaa
    25561 actgtaaatt tgatgccatt tatgttagtt gcaggagacc atgctataaa tgatatggca
    25621 ggagatgaag aagattcttg gaaaacaata ttagaagaaa atggatttaa cgtaagagtt
    25681 catttaaaag gactaggaga aaattcttat atacaagata aatttgttcg tcatgctgta
    25741 aaatgtgcag agaatgcgaa gttctatggt ataggagcag gaccaggaga tggttcttta
    25801 cttacgataa aagcggttaa tactttaaag aaactggata ttctatacac tccagaatca
    25861 aaaaagggtg gagatagtct agctttatca atagttagtg aatatttacc tgaatcagtg
    25921 gaaataaagt ctaggcattt cccaatgagt tttgatggga atgaaaaaag tttagcttgg
    25981 gataatgtgg cacaagaaat tatatcagat gttaatgatg gtaaaaatgt aggatttgtt
    26041 acattaggag accctatgat ttatagtaca tatgtatata taatgaaaag aatattagga
    26101 gaggtagaag tcgaaactat accaggaatt tcatcatttt ccaatatagc atctaatcag
    26161 aattttccat tagtcatgga taaagaagct ttagtggtaa ttccttgtac tatggaagat
    26221 gataagatag agtatgcttt gcaaaattat aattcaatag tattgatgaa agtatataaa
    26281 aattttaaag aaataataaa aaaattagaa aagtatgatt taatagaaca tgctatttta
    26341 gtaagtaatt cttcccaaga aagcgaaaat gtgtttacag atttaaaaga agcgcacctt
    26401 gaagataaga tatcttattt ttctactata ctagtaaata aagataataa aataaaatag
    26461 gtgaaagcaa tatttaaagg agacttaata tgaatatagt ggttggaact aggggtagta
    26521 atctagcgct tatacaaaca gaatgggtaa taaatgaatt aaaaaagaag tatccagaaa
    26581 ttagttttga gattaaaatc ataaagacaa aaggagattt aattcaaaat gtttcactgg
    26641 ataaaatagg tgataaagga ttgtttgtca aagaaataga gcaacaatta ttagatggaa
    26701 aaatagacat agcagtacat agtatgaaag atatgccttc ttatttagca gatggactaa
    26761 aatttgctca tacgcctaag agagaagacc caagagatgt tctaatactt agagaaggat
    26821 ataaaaattt agatgattta cctcatggag cagtaatagg aacaggaagt aaaagaagga
    26881 agtttcaatt attgaaacaa agacctgatt taaatatagt acaagttaga ggtaatgtgg
    26941 aaactaggat acgaaaaata aaagatgaaa atatgcatgg tattgtgttg gcagcatcag
    27001 gaataattag agctaatttg caagataaaa taagttctta tttacctgtt gatgttgtta
    27061 ttccagcacc tgcccaagga gctttagcta tagaaattag aagcaatgat agtacgatag
    27121 agggaatagt aaattcactg aaggatgaga atacagaaat ccaaatattg gctgaaagag
    27181 gttttttaga tggtgtaaat ggaagttgtc atattccaat ggcagcttat tgtgaaataa
    27241 tccaagataa aatacatctt actggcttat atggagattc agaaggaaaa aaagtagtaa
    27301 tcaagtctat agatggagat atttcttctc ctagagagtt aggtttaaag ttagctaaac
    27361 ttgtattgaa ggagtatgag aattatgaag ggtaaggttt acttagtagg ggcaggacca
    27421 ggtgattata aactcatgac tttaaaaggt ttagagtgta taagaaaatc ggatgtaata
    27481 gtatatgata gattagcaaa tagtagttat ttaagagaag ctaagcctga ttgtgaactt
    27541 atatatgtag gcaaggcatc aagtaatcat attttgccac aagaagatat aaatagaatt
    27601 atagcagaaa aagctaaaga aggaaaaatt gtaactcgtc ttaaaggagg agacccttat
    27661 gtatttggca gagggggaga agaagcagag actttagtag atgaaggtat agaatttgaa
    27721 gtggttccag gtataacttc tgctatagga ggtttatgtt atgcgggtat tcctataaca
    27781 catagagacc atgcttcgtc atttcatgta ataacaggtc atttgaaggg tgatgatagt
    27841 ggagaattaa actggaatgc tcttgcaaat aacaaaggta ctcttgtgtt tttgatggga
    27901 atttcaaacc tagagaaaat aagtgaaaac ttaatgaaag aaggaaaaga taaggataca
    27961 cctgttgctc ttataagttg ggcaactaga tataatcaaa gagttgttac atctacactt
    28021 gaaaatgttt atgaaactgc tattaaagaa gaagtaaaac cgccaactct tatagtagtt
    28081 gggagtgttg taggacttag agaaaagttg aacttctttg aaagtaagcc tttatttgga
    28141 aaaagtattg ctgtaactag gtctagaaat caaaatagtg tattggtaga aaaaataatg
    28201 gatttaggtg gaaatcctat agaaatacct actattaagg tagaaaaaat acaaaataat
    28261 attaacttag aaaatgaaat aaaaaatata aataagtata attatctaat actaactagt
    28321 aaaaatgccg tagaaatctt ctttgaaaag atatatgaaa tgaattttga tttaaggata
    28381 ttatctaatt taaaggtatg tgctataggt tctgctacat caaatgagtt gaaaaaaaga
    28441 ggaattatag cggatatagt accaaaaaaa tttgtagcag agtcactgta tgaagagtta
    28501 gcgcctattt taaatgatga cgataatata cttataccta gagcagaaaa tgctagagat
    28561 tatctggttg acaaacttaa ggctatttgt gaagtaacag aagtacatat atatagaaca
    28621 gtaatagatg aggagaaaaa aggtgagata ttagatattt taaattctaa tgacatagat
    28681 tatataacat ttactagttc ctcaacagta aataatcttg ttgaaattat aggaaaagaa
    28741 aatattaaaa tattagaaaa aataaaaaca ctatctatag gtcctataac tacagagaca
    28801 ataaatagtt tgggaataaa attgtacaaa gaatctgcta catctactat agattcgtta
    28861 gtagatttaa ttataaaaaa ttaattttgg aggcttgaat atgttaagaa gaccaagaag
    28921 actaagagca agtaaagcaa tcagaaattt ggtaagagaa actaaggtga atgtagaaga
    28981 cttaatatat ccactgtttg tagtagaggg ggaaaatata aaagaagaaa ttaaatcttt
    29041 accagatgtc tatcatttat caatagatat gctagaagac gaaataaaag aaataagtga
    29101 gttgggaatt gaacatataa tgttatttgg tgttccagat gaaaaagatg agtgtgcttc
    29161 acaagctttt aatgataatg gaatagttca aaaggcaatt agaaaggtaa aagaaataaa
    29221 ttcagatata aatgtaataa cagatgtctg tatgtgccaa tatacaagtc atggacattg
    29281 tgggatttta actgatactg gttatgttga taatgataaa tcattggagt atttggctaa
    29341 aatatctgta agccatgcta aagcaggagc tgatatggtt gctccatctg atatgatgga
    29401 tggaagaata caagcaataa gagaagcttt agatgaaaat ggattagaaa atgttggaat
    29461 catggcttat agtgttaagt atgcttcaaa ttattatgga ccttttagag aggcagctca
    29521 ttcagcacca tcttttggag atagaaaaac atatcaaatg gacccagcaa atagcaatga
    29581 agctataata gagactgaac ttgatatatt ggaaggtgca gatatactta tggttaaacc
    29641 tgcattatca tatttagata ttataagaag agttaaagat aattttgact tacctctagc
    29701 agcttataat gtaagtggag aatactctat gcttaagttt gctgtgaagg aaggtttatt
    29761 aaatgaagat gtaattcttg aatcaattac atctataaaa agagcaggag cagatataat
    29821 aataacttat tttgctaaag acattgctaa actgttgaaa aaataaattc tagtagtata
    29881 aagaatatca aaaagtatag gaattgtaat taactattat acagtagttt tgtttagaca
    29941 tttattgaag aataatagaa actaatttgc aatttctata ctttatttat gtaactaaat
    30001 aaaatttgga ggtagaagat gctatatcct attaatttaa aattagatga gttagatgta
    30061 gttataattg gtggtggaga agttgcatat aggaaatgta aaaatttttt agagtttaac
    30121 aaaaatgtta ctatagtttc taaacaaatt ttaaataaat tttatgattt aaaaggaaat
    30181 ataaaaataa taaaagatga ttataaagaa atatatataa aagatgcttc tgtaataatt
    30241 gctgccacaa ataatagaga gttaaatatg gaaataggat tatattgtaa agcaaaaaat
    30301 aagctagtga atgtagttga taatatagaa atatcgaact ttactgttcc atcatatata
    30361 aaaagaggag atttacttat aagtgtgtct actgggggca agagtccttc attatcatca
    30421 aagataaaaa aagaaattga agagagatat actgaagatt atgaggagta tttaaatgta
    30481 ttaggagata taagaaaaaa ggttataaaa aaatacgaag ataaaagtaa aagaaaagat
    30541 gtgcttaata tgttggtaac acttgaccta gaagaactta agaagtttga tatataaaaa
    30601 ataaaataaa aaatgttaaa aaggtgttga cggattattt ttaag
//
LOCUS       DAMJYC010000044        30634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJYC010000044 DAMJYC010000000
VERSION     DAMJYC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.045"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            231..1310
                     /locus_tag="PC472_002109"
     CDS             231..1310
                     /locus_tag="PC472_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2727435.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC472_002110"
     CDS             1634..2002
                     /locus_tag="PC472_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2727436.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2916
                     /locus_tag="PC472_002111"
     CDS             2119..2916
                     /locus_tag="PC472_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2727437.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3315..4205
                     /locus_tag="PC472_002112"
     CDS             3315..4205
                     /locus_tag="PC472_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2727438.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4968..5411
                     /locus_tag="PC472_002113"
     CDS             4968..5411
                     /locus_tag="PC472_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2727439.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMHALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5583..5753
                     /locus_tag="PC472_002114"
     CDS             5583..5753
                     /locus_tag="PC472_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727440.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            6126..7247
                     /locus_tag="PC472_002115"
     CDS             6126..7247
                     /locus_tag="PC472_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2727441.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7452..7940
                     /locus_tag="PC472_002116"
     CDS             7452..7940
                     /locus_tag="PC472_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2727442.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            8026..8433
                     /locus_tag="PC472_002117"
     CDS             8026..8433
                     /locus_tag="PC472_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727443.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8434..8652
                     /locus_tag="PC472_002118"
     CDS             8434..8652
                     /locus_tag="PC472_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727444.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            9595..9900
                     /locus_tag="PC472_002119"
     CDS             9595..9900
                     /locus_tag="PC472_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727445.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            10308..10997
                     /locus_tag="PC472_002120"
     CDS             10308..10997
                     /locus_tag="PC472_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727446.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            10997..11914
                     /locus_tag="PC472_002121"
     CDS             10997..11914
                     /locus_tag="PC472_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2727447.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            11987..12913
                     /locus_tag="PC472_002122"
     CDS             11987..12913
                     /locus_tag="PC472_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727448.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            12910..13590
                     /locus_tag="PC472_002123"
     CDS             12910..13590
                     /locus_tag="PC472_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727449.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            13688..14023
                     /locus_tag="PC472_002124"
                     /pseudo
     CDS             13688..14023
                     /locus_tag="PC472_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            14967..15617
                     /locus_tag="PC472_002125"
     CDS             14967..15617
                     /locus_tag="PC472_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727450.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            15933..17999
                     /locus_tag="PC472_002126"
     CDS             15933..17999
                     /locus_tag="PC472_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2727451.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKDAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMNEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            18016..19971
                     /locus_tag="PC472_002127"
     CDS             18016..19971
                     /locus_tag="PC472_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2727452.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKASTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSEPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            19987..21177
                     /locus_tag="PC472_002128"
     CDS             19987..21177
                     /locus_tag="PC472_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2727453.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            22356..22730
                     /locus_tag="PC472_002129"
     CDS             22356..22730
                     /locus_tag="PC472_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727454.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            22732..23589
                     /locus_tag="PC472_002130"
     CDS             22732..23589
                     /locus_tag="PC472_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727455.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            23592..24230
                     /locus_tag="PC472_002131"
     CDS             23592..24230
                     /locus_tag="PC472_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2727456.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            25236..25970
                     /locus_tag="PC472_002132"
     CDS             25236..25970
                     /locus_tag="PC472_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2727457.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYN
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            27309..28565
                     /locus_tag="PC472_002133"
     CDS             27309..28565
                     /locus_tag="PC472_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2727458.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSEVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            28628..29821
                     /locus_tag="PC472_002134"
     CDS             28628..29821
                     /locus_tag="PC472_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2727459.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            30022..30402
                     /locus_tag="PC472_002135"
     CDS             30022..30402
                     /locus_tag="PC472_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2727460.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT        11690 a         3219 c         5270 g        10455 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatactttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgagcactat ggaagaaaaa tacatcttat ctgaccgagg taaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttagtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atacccatta
     2941 ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gtataataca aagcatgata tatataaatt
     3061 tttaagcgaa atacatcaaa agaaggatta gaattagtgg agacagaaca gctattatag
     3121 attataaata aatagtttta aaggagaggt actacgaata tagaggaagg taaatcatac
     3181 gttattcaag tattataaat gattagttta aaagtactat cgaataggag tctccatatt
     3241 taaaccttag taaaggtagt taaagtagta gtttaaaaac taaaaaatta tataaaggaa
     3301 ttaggtataa gaaaatgatt gagagtatac cagcaaaaca aattttacaa aaagtgaaat
     3361 ttgacaatac aagatggttt ggaattgact ataatatgaa tctctatcgt ggttgttctc
     3421 atggatgtat atattgtgat agccgtagca caatatacaa catagagaat tttgacaaag
     3481 ttagatataa agaaaatgtg atagaaattt taagtaaaga attgcgttca aaaagaaaaa
     3541 aaggggtagt aggcattgga gctatgtctg acacatataa tccttttgaa aagcaacttt
     3601 gtattacaaa gcaggcacta gatttgattt cggaaaatca ttttggagtg tctattgata
     3661 ctaaaagtag tttggtggtt agagatatac cgattttgca gaaaattaaa aaaaataatt
     3721 ctgctattgt aaagttgact ataaccactg caaatgatga attatcaaag aaaatagaac
     3781 cttatgtaaa tccaagttct gtacgatttg aggcagtgaa agaacttaat gattctggaa
     3841 ttttttgtgg tatattattg actcctatgc ttccattttt gactgataat aaagatgaaa
     3901 tacgagcaat tgtagaaaaa gcacacaagg ctaatgcaaa atttatttat tgcatgtatg
     3961 gtgtaacaat gcgtagtggt cagagagagt ttttttatga gcatttaaga gatatatcac
     4021 caaaattggt acttaaatat cagaaaacat atggtttaaa ctatgtatgt actgtacaaa
     4081 ataaagattg ttgtgagaag ttgttaagag aagaatgtgc acgttatggt attttgacgg
     4141 atatgaaaga gattataaaa gcgtacaaga ggtcggagtc atatattcaa ataaaatttt
     4201 tttaagatag gctttggatg agatataata gtggtgattg atgtgttgat attatagttg
     4261 attgttagag aggatattat agatgaatat tagtattgat attttaggta attgctagag
     4321 ctgatattat agatgaatat tagtattgat attctaggta attgctagag ttagtattat
     4381 agatgaatat tagtattgat attctaggta actgctagag ctaatattat agatgaatat
     4441 tagtattgat attctaggta attgctagag ctaatattat agataaatat tagtattgat
     4501 attctaggta attgctagag ctgatattat agatgaatat tagtattgat attctaggta
     4561 actgctagag ctaatattat agatgaatat taatattgat attctaggta attgctagag
     4621 ctgatattat agatgaatat taatattgat attctaggta attgctagag ttagtattat
     4681 agatgaatat tagtattgat attttaggta attgctagag ctgatattat agatgaatat
     4741 taatattgat attctaggta attgctagag ttagtattat agatgaatat tagtattgat
     4801 attctaggta attgctagag ctgatattat aatttattat tagtattaat attctaggga
     4861 attatcacta tattttaaaa agtaaaaata tatatcaaaa tgaactaaat aagtactgta
     4921 agaagtttgt taaattaagg tttgaataaa gtgagggtgg agaacttatg atagaaataa
     4981 aaactataga aataaataaa aaaagtcaaa ttgacctttt aatgttagcc gacccatgtg
     5041 aagaaatgat tgataaatat ctcaataaag gaactatgca cgcattgtat gataataaag
     5101 aacttacttg tatagcagtt gtaaatgaaa tttccaaaga aatatgtgaa ttaaaaaaca
     5161 ttgctacata tgagcatttt caaaatatgg gttatgcctc aaagatgatt tatcatcttt
     5221 tagatgttta tagtaaaaaa tattcatcaa tggttgttgg tacatctgaa agcggagttc
     5281 ctttttatga gaagtttgga tttgtttatt cacataaaat taaaaacttt tttgtagata
     5341 attatccaga accaatcttt gaaggagaat tacaatgtgt tgacatgttg tatctatcat
     5401 ataactttta gataggataa tatttttagg ttaatataat taattaattc atataattat
     5461 cggaattata atttataaat tatgtataat aagagtataa tttatttaga agctgttaca
     5521 ttgtattata ttcaaataat atatataata acttgtttat tttgaatttt ggaggattaa
     5581 ttatggattc aaataataat actataaaat caactgttaa aaagggtatt tcttttggtt
     5641 cttgtttagc aatgattatt tcttatactg catggaaatc tattccatgg gctatttttc
     5701 atggcttaat gagttggata tatgtacttt attattgggt taagtatgca tagattaaat
     5761 taataaataa gtatgtaata ttgtaaagtt taagaaacaa aatgtaaggg agtgtctcga
     5821 agtgaacttt tagtttataa ggcactcttt tttgtattaa ataaaatata ttttcattat
     5881 ttaaacaatg aaaaagatga gtatctctat tttaatacat acccttatat tcattcattt
     5941 atcaacaata gttttaaaaa gaaagctatc taaaatctat tgtaatctaa gtgcttctct
     6001 ttaaagtctt aactttagta atgatatata agtttataag gttcttattt caataatgca
     6061 aattttactt agaagttcag actttggtat gaagaaatgg gtaataataa aaaagaggtg
     6121 ttgatatgaa agaaaaatta gaagaaaaat ctaagattat taaaacatca aaagagtata
     6181 aacgaactat aattgaagaa aaggacagag aatggaccaa aaaattaaat gacatactta
     6241 gtagaggtga tttttctaat gaaaaattag ataaaatcaa ggatttaatt ccaaaagaaa
     6301 tattgactag tgaaaatgtt ggagaagttg taactgaaga tgggtatgct aagcctgagt
     6361 atgatgaggt atttaaatca ttgtttactt taaataatga ttacgattta cttgcaaatt
     6421 ttataaatga tatcctcaaa gatgctccat atgctaatag gaatatcaaa cagttcactc
     6481 aaataaaaag aataattaag gttgaaaccg acccaacaat taattacatt ggtgagaaaa
     6541 gacctagact tgatattttg gctgaagatg aagaacgtaa ccatataaat attgagatgc
     6601 aaagagctct tgaagaggat tatttggaaa gagctgaata ttatcttagt cgtgtacatg
     6661 gtagaaagtt agaagaaggt aaagaatata aagaaattgg aaaaactgtt ggaatacata
     6721 tactaaatca tgtaaaatat aatcacatag aagattatgt aaattgcctg agattaacaa
     6781 tggacgggca tccagatatt ttttctagta aaactgcatt atattttata gaacttccaa
     6841 aaattcgtaa atccagttgt atagcaaata gagttctaat atggggtaaa cttattgata
     6901 atccttctca tatagatatt agaatactat ctaaaacgga ttatgttatt aaaagagcac
     6961 ttgatagatt aaaagaatta ggtagtaatg aggactattt attaaattta aagagaggtg
     7021 cgtatattat gaataaaagt agaaatttta aagaagaaat ttttcaagaa ggtattgaaa
     7081 aaggtatcga aaaaggtatc gaaaaaggta ttgcaaaagg gaaaagagaa gagaaattta
     7141 atatcatcat aaaattaaaa aataaaggat acaatttgag tgaaatatgc gatattatag
     7201 atgatttgaa taaatcagag gttgaaaaag tctataatca aaactaatac tatgattgag
     7261 atggcactag ataaatacaa agcttcctct tttaaattta aattaatata aaactttgct
     7321 atttaaatat ggtagatata aagagatata taaataatag gtacaaagag gtgtataaat
     7381 aaaggagata attcaaaata aaagtttgaa ccatctcctt cttatagaaa aacaaagggg
     7441 ttgatttata tatgaaattt tatactatac agactttaga attttggaat aacaataaaa
     7501 acaataaata tctatcaaat gattattctt ttatattaga agactttgat attccataca
     7561 aatggatgta tagtcaaatg acaaatagaa tatatgaatt tgatgatagt atgatatggg
     7621 tttggacgaa gagacctgat ttaagatgta gtggttattt agaaaaagga aaaaaaggtg
     7681 ttttacttga aatagaaata agtgaagata atgtgttatt atcagaattt gatttatggc
     7741 atttaccttt aatggatatt gctgtagagc tttatgaaga tgaacaaatt gaaaagaagg
     7801 taagttggga aagaatattt gacttagaac tgtgttcaaa attaaatgaa gttaatatag
     7861 aagatttgac attgcaagga gtgacatcta aagtgcctct atctaatgta aaattaataa
     7921 aagaatttgt tgcaaaataa tatttttatt gaaatttatt caaaaaagtt atatatttat
     7981 aacaagaggt gttataaata cataactttt taagggggat tttatatgtc taaacaacaa
     8041 actggtatta aatcaacact tatttcagaa atattgggaa tttcttattg tattggagct
     8101 acattagtaa ataagtattt ttttaaagga agtagtattg aatatcttcc tataattatt
     8161 tgggtattag ctatgggagc tcagtgggtc tataataaaa agtatgatga tttaattgat
     8221 gaggcatcaa aatgtatact atcaagagtt aatgatattg caataaaagt attgtttttt
     8281 tctgtagcta tagtatctat atttcttgta actttggaca taaatgtgtc aaatttaaat
     8341 ataggaatgt ctttattagt cattttgttt attcaatcct tgttgaagtt aattctattt
     8401 atatattttg acagaaaggg gatatacaat taggtgtcag tattactaaa gacaaaaatt
     8461 aaatcctata gagaaaagaa ccttatgaca caaggtgaac ttgcaaagtc agtaggagta
     8521 agacgtgaaa ctattgtaca tttagaaaat ggtcgctaca atccttcttt aaaactggca
     8581 atggatattg ctaaggtatt tgatacaact gtagaagagt tgtttgaatt tattgaagaa
     8641 aataatattt aaatttcatg tattacttat gcattagaga atgaattaat agataaaaaa
     8701 ttcaagttta tgtattagag aatgaattaa ttataggtaa aaaaccaagc ttatagatta
     8761 gagaataaat gacttataga tgaaaaacca agctcataga ttaaagaatg aatgatttat
     8821 agatgaaaaa gaaagcttat agattagaga atgaattaat tataggtaaa aaacaagcta
     8881 atagattaaa aaatgaatga tttatagatt aagaagtaag cttatagatt agagaataaa
     8941 tgacttatag attaagaagt aagcttatag attagagaat aaatgactta taaataaaaa
     9001 ccaagcttat agattagaga ataaatgact tataaataaa aaccaagctt atagattaga
     9061 gaatgaatta attataggta aaaaccaagc ttatagatta aaaaatgaat gatttataga
     9121 tgaagaacta aacttataga ttaaaaaata aatgacttat ttatagatga aaaaccaaac
     9181 ttatagatta aaaaataaat gatttataga tgaagaacta aacttataga ttaaaaaata
     9241 aatgacttat agatgaaaaa tcaagctcat agattaaaga ataaataact tattagatga
     9301 aaaaccaagc ttatagataa gaagtccatg cttataagcc agaaaatgaa tactttaata
     9361 gattaaaaaa tacatatttg tgaattatag aatgaatgac tccataaact agaaaattta
     9421 taataatatc tatatgaaaa ttagaattat tttatcacaa aaaaattaag ttaaataaat
     9481 atgaaattac ttccaaatat acttccaaaa acatgtaatc gcatatataa tataattata
     9541 aaaataataa atatttttat agtagtagtt aattattaat aaggaggggt caaaatgcct
     9601 agcatgatta tggaagttgt aggagtacta tttatagcta ttggattaat cttgacattc
     9661 attgaaataa aaggaaattt cccttctttt tatggttttg gtcaaaattc tatagggaaa
     9721 aatgctgtag gtattatagc agcaggaatt gctttaattt tctttagaaa tggcatagat
     9781 agcatagatt ctatagctac ttatggtgta tttttagcaa tagaacttgt tgtaatagtt
     9841 gcatatttct taataagaaa aaaatttact aaagaagata aacatttaag aactaattaa
     9901 ctaaattatc tactataaaa ttggaaaatt aaaaacaaat aaaagtttgt ttttaattat
     9961 ttaaaatcca aatttgatta tttttaaaga aactatgttc tgtataaaac tagaatgtgg
    10021 tttctttttt atgtataaaa tattttttag caaaagttaa aaggtgtttt ataatataaa
    10081 atagttaatt gtagatgtta ataaaagaaa attatgtata tttcagataa agataagaga
    10141 gtatactata ttttaattct tttttcaata aagcttattg aaaagaactt taacttagaa
    10201 gagtatcacc aaattgcaga tacaatatca taagcagtta tttattgaca acccttttta
    10261 ataaatatat catttgaagt atactgacga aaaggagttg aataataatg gacatcttac
    10321 tggttgaaga tgataagaat attcgagaaa tggtatacga ttttctgatt agtgagaatt
    10381 actctgttaa aactgccatt gatggaaata cagctattga aatatttaaa aattattcat
    10441 ttgatattat tttactggac ttgatgttac caaagtctag tggttttgaa gtattaaaat
    10501 atatacgaag tgtgagtgtt gttcctgtta ttattactac tgcgaaagat agtgacacag
    10561 ataaaatgct agggcttaat ctaggtgcag atgattatat tacaaagcct ttttcaatcg
    10621 tagaattgct tgcaagaatt aaagctaata ttagacgtgc aacagtgtat agcagttcaa
    10681 gaaactctca agatatggtt attcggtata aggatttgat tataaacata tcaaaatatg
    10741 tagtttcaaa gaatggaatt gacttaaact taacacatac agaatttgaa attgttaaat
    10801 tactagcgac taatcaagga cgtgcttttt caaaggaaca attatacaac gctatttgga
    10861 aagaacctta ttatggaaat gaaaatgtgc taaatactca tattaacagg ttaagaaata
    10921 aattgtctga tggtatagaa aaaaataatt atattaaaac tctttgggga attggctaca
    10981 aaatggagga agaataatgg agttattttt agttgtaatt tcgattgtac tatttgctct
    11041 ctacttaaaa gaacgaaata aatcaaagaa aatggaaagt gattataagt atatcaattc
    11101 aagacttcgt gacataacaa atgatgatga aattaattat attttagtac catctgatat
    11161 ttcgattgta aaagaaacag cagtagatat aaataaactt gttgaaaagt tttacagcag
    11221 gcaaattaat tataatagaa gtgaaaaagc gatattgcaa atatttacaa acatatcaca
    11281 cgatttacga actcccatca cagtattaaa gggttatatt gaaatgctat atcttcaaag
    11341 tcaaaaagaa gaattatctt cttcaatgag agcaactgtg gaaaaaatgc aaacaaattc
    11401 aaatgagctt gttaattcaa taaataacct gtttaatatg gcaaaaatcc aatctggaga
    11461 tatgatatta aatcttcaaa aatccaatct aacacaatta tgtcatgaga ttattttaga
    11521 gttttatgat ttgctagata aagaaaactt taatgttgaa gttaatattg aagatagacc
    11581 tatatatgca aatgttgaca cagaagcatt acaacgtata ctcaaaaatt taattgataa
    11641 tgctataaaa tatggttcag atggtagatt tttagggatt tcactatatg aaaaggaaaa
    11701 acatgttttt atagatattg aggataaagg ttcaggtatt tctgaaactg aaaaagagca
    11761 tattttcaca agagcttata ctgctgacag aaaaaaaggc aatggtttag gtttggctat
    11821 ttctcaaggg cttgtaaact ctatgggagg ttcaatttat gtaaatagta gacctaatgt
    11881 aaaaacagtt tttacggtaa aattgaaaag ttagaaaaaa gttagattta aaagagagaa
    11941 aagataattt tattttgtat actgttttta gaaaggaggg agtatgatga actatatcat
    12001 agagacccat caattaaaga aaatatataa agataaagct gttgttaatg ctgttaatat
    12061 tcatgtgaaa aagggagaaa tttatggatt tgttggacca aacggggcag gtaaaagtac
    12121 agtaatgaaa atgttgctaa atcttgttaa tccaaattct ggagaaatag ttatgttcgg
    12181 taagaaagtg gcagaaactg attttgaaat attaaaaaag attggtacta ttattgaaaa
    12241 tccgtattta tatgagaatt tgactgctaa acagaatctt gatttacact gtgaatatat
    12301 gggttattat aacaaagaac atatatcaga ggtattggaa tgtgtcggat tgtcaaagca
    12361 atcaaacaag aaagtttcaa aatattcttt agggatgaaa caacgtcttg caatagctag
    12421 agcaattctt actaaaccag aattattgat tcttgatgaa cctataaatg cactcgaccc
    12481 agaggggata agagaaatgc gagaattatt ccgtaagtta aatacagatt atggaataac
    12541 tatatttatt tctagtcata ttctttcaga ggtagaacaa attgcggaca ctattggaat
    12601 tattcaagat ggtaaactta ttaaagaaat ttctatgtca gatatacaca aatatcaaac
    12661 agattatgtt gaagtagatg tagataatgt ggagcttgct ggatacttgt tagaaaaaga
    12721 atttggaata aaagacttta aaattacttc ggaaagttgt attgaaatat atgatttacg
    12781 aaaagatgta aaagaaatat ccaaaatatt tattcaaaat caaattggta taaatagcat
    12841 tggacgtaaa caaagttctt tagaagatta cttcttccaa actacaggta caggaggtaa
    12901 agaaacacta tgatacattt aatgaaacta gagctaaaaa aagttgggct aaaaagatat
    12961 tttttatttt caatgctggg gattttctta tgtatgtttt ttgtctttac tggattgaat
    13021 gataaatcta cttcaatata tgattatgat gttacattta ggacaatcag tttatttttc
    13081 tgtttttact atattatatt atttgctgtg ttgaatgtgg catatataat aaatgaatat
    13141 acaaataaaa caatcttgat tttattctct tatccacttg accgtaaaaa acttatttta
    13201 tcaaagttac ttttgattac gactcttatg atatcatcaa tgatagttgg atatatctgt
    13261 tgttttacat tcgttgttgt gttggataag tattttaatt tagttattgg agattttcat
    13321 atttcaattt tatcttattg gataccagga gcaattaaaa gtattattgt attttgctca
    13381 ttaggtatat ggacattagt tgttggaatg ataagaaaat caatacctgt tacccttgtc
    13441 agttcaatta tttttatcaa tttaagacaa tttattttag ctggtacaaa tacaactcaa
    13501 gacagtttga tttctgtttt tattatagtt ggtataactg tgattggtat atattatata
    13561 ctaaatcata aagttactga aattgactag atactgtggc taggaaggta gttattaatt
    13621 gtatcttctt gggataataa tgatttgcta ttatatcagc tattattaat attattagag
    13681 ttttaataat attgcacaac catttgaagt tgagccaaag cttgatgaat taattgaatg
    13741 gtactatagt cagagtgaag tatctattaa agttatagct gaatttcatt atagatttga
    13801 attgatacat ccatttcaag atggtaatgg tcgcataggt agatttgtta tgctaaaaca
    13861 gatgttagaa aataatcttc ctataaaaat tgtatcttgg gatagtgagg aattgtatag
    13921 aaactcctta aattcttgta gtttaggtaa ttatgtccct ttaattgaat atttatcaag
    13981 tttagaagat tttagagaag tatataaaat gttatggaaa taattgtttg cagcaaaaaa
    14041 agtttttata taaatatatt tttatctatg ctatttaacg ctaatttctt agttattctt
    14101 agcttggact atactcacta ttttaactat ataaaatagt ggatatagtt taactatctc
    14161 tttataacta tttttatgta tcttagttgt ttcttaatat ggggacagtt cataaaacta
    14221 aaagtgattt ttaattttat catttataat ttaccattac atcagaaaaa tacttatcat
    14281 tagaattttc ataaaaggta cctatttttt tcaattaact aaatcaattc tattagtctt
    14341 aatctttcgt atttcaaatt gataatataa gaaaataaaa aataaaaaat tttaaatttt
    14401 ttatttattg cgaaaaaaat aaaaaggtgc tatacttttt gtaacatatt attcaaatta
    14461 ttaagagata gtaaacattt atagtttaat ttgtagtaca ctagcatgat ttgcagaaca
    14521 atgaattaat ctatagtata atagtttaat ttaaagtaca acgatttaat ttatagtaca
    14581 ctagcatgat ttgaagtaca acgatttaat ctatagtaca ataacttgat ttgcaataca
    14641 atgaattaat ctatggtata atagtttaat ttgaagtaca atgaattaat ctatagtata
    14701 atagtttaat ttaaagtaca acgatttaat ctatagtaca ataacttgat ttgaagtaca
    14761 acgatttaat ctatagtaca ataacttgat ttgcaataca atgaattaat ctatagtata
    14821 agagcatgat ttacagtaca ataatttaaa ttgtagtaca aaagtactat caaataagaa
    14881 tttctacttt aaaaatatta ctaaaagtaa ttaaagtcca gtaattcaat ataatatggc
    14941 aaaagaatag aaataaggtg agagtagtga aagtaaagtc aaatttagat gaaattttat
    15001 atcataagat tattgaaagt cttattcgtg gtgaatactc tgtaggtcaa aaaatattac
    15061 taaatgacct ttgtgaaaaa tttgaagtaa gcagaacacc agttgtacaa gcagtaaaaa
    15121 tgctaaataa agatggagtg cttactatta tgacaaatgg taaagtgtac gtaccagagt
    15181 atgaatatga tatggtaaaa caagtttgtg aaacgagaac actgattgaa acatatgctt
    15241 tagaaaaaat gatgcaagaa gaggaagagg tttttcaaca aaagctagat gtaataaaaa
    15301 gatactctga taaatgcgag gagtactatc gtcaagaaaa atctgtagaa cttgcactag
    15361 cagatttgga attacataaa gccattgtag aaggggcaaa taatgagatt ttaaatgatg
    15421 tatatgttgg tatacaagga agatttatag tagtgaacta tttaattcga ccattaaaaa
    15481 atcgtaatta tgaaggaaca gtacaagacc attttgaaat attaaaattc attgaagaca
    15541 gagatacaaa aaaagcagta gataaactaa gaaatcatat tcaaggaact attaaaagat
    15601 tctgtgaaga tgaataataa gttttgaagt tactatatat acttatctat taagttgcta
    15661 aatgtagtct aagtataatc taattatagt ttaaacataa tctaaatata atctaataaa
    15721 tataatctaa atatagtgta aataagtcta agtatgaatt aaatatagat taagtatgga
    15781 ctaagtatag attaagtatg aattaagcat ggattaaata tactttaaat tacaaatatt
    15841 acaaggaagc cagaaggctt cctaaaaaaa taacctttat aaaatttttt attttaaatt
    15901 taaaattttt tattttaaat ttaaaataaa aaatgaaaga ggtggtaggg agtttgaaaa
    15961 aaattactaa agaaaggata gaaaatacta tgaaaaagct atttacgagt gaaacatttt
    16021 tggaatatca atttataagt gattgccaaa tatcaccaaa tggaggatat acagcattta
    16081 ttgtaaaaaa agctgatatc aaagagaatg gttacacaag ccaagtttat atattaaaca
    16141 ataaaactgg cgaattaaaa caaataacgt ctataaactc tgttggagca tatgcctggg
    16201 aagatgaaaa tactatcttg ttccctgctt tgcgaaatga aaaagtaaaa gatgctgtca
    16261 aaaacggtaa gcaatgcatg agttactatg ctttatcttt aaatggtgga gaagcagaag
    16321 aactttttag actccctatc aaaggtggta aattgaatcc tataggaaaa ggtttgtatg
    16381 cagttgttga ttcttatgac aatgaccgac ctattgtaga gggattgcca gaagaagaac
    16441 aacagaaaaa aataaatgct tataacaaaa gacattatga acatttcaaa gaaattcctt
    16501 atgctgtcaa tggggaggga tatattagca gaaaaagaaa acgcctatat gtatacgaca
    16561 gtaatataaa tgagttgaag gcaattacag cacctatgtt taatgttgta ggtatgaaaa
    16621 taagtgatgg aaaaatctta tatatagggc aagagtttaa agatgtaaaa ggtttgaaaa
    16681 atggggtcta tgtatttgat acaaacacaa acacaaatgt ctgtatatta gaaaaagata
    16741 agtatattat aaaaggtttt gaattatatc aaaatcaagt gatactaaac ctaactgatg
    16801 ctttatctta tggaaatgga gaaaatggag atttttatac tattgatatt gatacgaaag
    16861 aaatgaaact tctttcaggg catcaacatc attgtattgg aaatacagta acatctgatg
    16921 ttaaaatggg tgctggtcag acaacaaagg tagatggaga atatatttat tacacttcta
    16981 cagtagatat ggactgcatt attgaaagaa tacatattcc aacaggaaaa caggaaaaag
    17041 taacacagac aggttcagtt gattttatag atgtaaaaga tggcaatatt gtatgtgtgg
    17101 gttgtttagg aaatggatta ccagaagttt atacagtaga gaatggaaca ttgtgtaaga
    17161 aaacacattt aaatgaccat attttagagg aatataaaat ttctgtgcca gaatacatag
    17221 agtcaaaagg aagttctaaa tgggaaatac aaggatacgt atataaacca gtagattatg
    17281 aggtggggaa aaaatatcca gctgtattgg cgattcatgg aggaccaaga ttgacatatg
    17341 gaccatattt tatgaatgag atacaggtat ttacaagtgc aggttacttt gtattcttct
    17401 gtaacccaag ggggtctgaa ggtcgtggca atgcttttgc agatattagg aaacagtttg
    17461 gagatattga ttatatagat tttatggaat ttacagatac tgtacttgaa aaatatacag
    17521 acattgataa aacaaaattg gctgttgaag gtggaagtta tggtggattt atgacgaatt
    17581 ggataattgg tcatacaaac cgttttgctg ttgcttgtgc tcagcgttct attgcaaact
    17641 ggagtggcat ggagggtaca acagatattg gttattattt ctgcaaagga caaacaggtg
    17701 cttctcatat ggaaaaccac gagcttcaat ggaaacagtc acctcttgct tatgctgata
    17761 aatgtgttac accgacttta tttttacatg gtgaaaaaga ctatagatgc tatatgcaag
    17821 aagcattcca gatgttttca gcattgaaaa ttcatgggtg tcctacaaaa ctttgtctat
    17881 ttgaaggaga aaaccatgaa ctgtctcgtt ctggtagacc aaaacaaaag ctacaaagat
    17941 tagttgaaat gctagattgg ttttcggtat atataaaaaa agataacata ggacagtaga
    18001 aaggagaata taaaaatgga aaaaataaag attgaaagtt cagatatttt tgaatatata
    18061 ttccctcatg atatttcttg ttcacctgat ggaaagcata ttgcttatat tatatcaaat
    18121 atcaacgagg aaaaagactg ctatgaacat gacttatatg tgatggacat caaaacagaa
    18181 aaacaaatac atatgactca aacaaaagat gtaacatcat ttagttggat ttctaataca
    18241 gaactacttt ttacatctaa aagaaataag ccaaaagcaa gtacaacaga tttttacact
    18301 atttctatag aaggaggaga agcaaaaaaa gcattttcta ttccaaaagc ttgtagcgta
    18361 cctgtatcac ttggaaataa attgtggtta ctgacaacaa aaaatccaac ggattctaaa
    18421 aaatcagaac cagatagggc agtagagggt gtagattatt ggacatttac agataaacct
    18481 tttataagag atggagaaaa tttttctcaa agaagacgtg taacattaga attatatcaa
    18541 gaaggtgaaa atataacaaa agccattaca ccaaaatttt gtgaagtagc aggtgtagac
    18601 gtttcttctg ataaaaacag aattttatat actggtcaga tttatgaaga ttgtgcaact
    18661 cctttttctg gactttggga gtatcatata gacagtggtg aaaccaaaga acttgtacca
    18721 caaggaaagt accaaataag tttggcaaaa tatattggca aagataaagt aatgttgcaa
    18781 gcttcaacat tagaccgttc tattacacag aatcatgata tttttatact tgatttgagt
    18841 actagtgaaa taaatatgat tgctagtcca gatggtatgt atgctacatt actggatgta
    18901 gatgcagtat atggtggtgg acgtagtaat aaagttatag gagacaaatt tattggtgca
    18961 agaatttgca gaactatgac agaatttaat gagtttgata caaagacagg taatatacgt
    19021 atcatcacaa aagtagatgc ttttacatct tttgatattt acgacaacac catgtacaca
    19081 gtaatgttga aagattatga gttagcagaa atatatagca ttgatatgac tacaggtaca
    19141 atgaaaaaaa tgactgcttt ttctgaacca tatttagata ctcataaggt ttcattacct
    19201 gaaaaattga cgtttgtagc aaaaaataaa gaagaagttg atggttttgt tataccgcct
    19261 atagatgcta aagaaggcga aaaatatcct gctgtattgt tcattcatgg tggacctaaa
    19321 tgggcatatg gctatatgtt tacacacttg aagcagtgtg taacatcaaa gggaatgtat
    19381 gtgatttatt gtaaccctca tggtggtgac ggttatggtg aaaaattctt agaaatggta
    19441 gaaagatggg ggtatgttga ctatgagcat cttatggaat ttgtagatac ttgtattgaa
    19501 aaatatcctg ggattgatgc agaccgctta ggggttgcag ggggaagtta cggtggttat
    19561 atgacaaact ggataattgg tcatacagac cgtttcaaag cagcagtttc tcaaagaggt
    19621 attagtaact tgattacagc ttctttaatt attgactttg gggatagaat tatgaagcag
    19681 acttgtggag ataagacgcc ttggaatcat gaggaggttc tttggaatca ttcacctatt
    19741 aaatatgtga aaaatgttaa gacaccaaca ttgttcttac atagtgatag ggattataga
    19801 tgttttatgg gggatacttt ccaaatgttt acagcattaa aacagcttgg tgtagataca
    19861 gaaatgtatt tgttccacgg agatacacat ggattgtcta gaaatggaag accatctaat
    19921 agaattgcaa gagcaaatgc tattgtagat tggtttgaaa gatatttata ataagggggt
    19981 tattctatga gtgatatgtt attatggttt agtgtcgtat tagttttatt tgctattggg
    20041 gatttaattg caagcaaaac aaaagcaaaa gtatcagctg tatttgttac actactttta
    20101 tttttgattc tatttgtcac aaaagcaata ccagctgata ttattgaaaa agcaggtatg
    20161 accgcagcag ctagttggag tgttccaatg attatgttta gtatgggaac aatgttgaat
    20221 gttaaacaat ttatagacga atggagaaca gtattgacag catggcttgg aattgtagca
    20281 gttatagtct gtgtctcatt atgtattcct ttgtttggaa aatcaacagt attgacaagc
    20341 ataccagtta taaatggagc tttacctgct actactatta tgacacaagc agcactagaa
    20401 aaaggactga cacttgcagc agctacagca acagttgtat ttgcaattca gaagtttatt
    20461 ggtacaccaa tagcttcaag agcagcgtta caagaggcaa accgtctttt agtagaatat
    20521 catgaagcaa aatcaaaagg cattgattta gcaaatgttg atactgataa gaaagaagca
    20581 gagaatacag gcacaaaagt aaaacaagct ttctgtgaaa aatacgataa atattattct
    20641 acaaatgttt gtatattttt tatagcatta ttctcttatc tagggtatga actttcagag
    20701 attattcatg taaattattc tattgtatgt cttgttgttg gtgttattgt aacacgtatt
    20761 ggtattgtac caaaagacat tttggaaaaa ggaaaaatca agggatttat caacatggtt
    20821 gtgtttgcag ctgttattcc ttctttagca aaagtttctt tgacagattt aattagtctt
    20881 tttgtaccaa ttgtaggtat gtttgcagct tctatcattg gtatattcct tatgatgaag
    20941 gtgttaccag gttggaaaat tatcggttct aaacctcttg cttttggagt agggttttgt
    21001 cagatgttgg gattcccaac tacatattta atttcaaatg aagtgtgtaa tgctgttgga
    21061 gaaacggaag aagaaagagc atatttgatg tctaagatta tgcctaagct ggtagttggt
    21121 ggtatggctt gtatgatttc tattgtggta gctggtctta tggtaccaat gttgtagata
    21181 ggtttcatag ttaaaaaagt ggtgttaatt tataggagaa gctaaaaagt agtattaatt
    21241 tataaataaa gctagaaagt cagtattaat ttataaacga attttatatt agttaattta
    21301 ttataagaat attttctttt tagcattata atcttgattt tatcaagaaa cagatttttt
    21361 atttgctatt ctaatagtta cagtttattt ttattttcta aaacaaaact ttaacttgat
    21421 ttatctataa atagggtatt taataatatt tttggaaaat ctttagaaag aattaaaaag
    21481 aggagaaaaa agggagtgcc acaaagtgaa ctttttagtt ctaaggcact cttttttgta
    21541 cgaaatcgaa tatattttca aagttttaaa agtgagaaag agacttatct ctagtttgag
    21601 atagccaatt ttaattatta aaattatttt ttgttttatt tatcaaagag tattcgtgtt
    21661 ttagaagtta gtatttttta ttttatatat acaaggtttt ccgtttctaa tgttttcttt
    21721 tttaaaataa ttagggtatt caattatggt aaaaggtgga tggtattttg cagtgatatt
    21781 atttaaagca cagtattgaa tgttgaaatt ctcacatttt agagtattat cttttttaat
    21841 caaaaaaatc ttctatactt atagcaaggt tacttctgca aatattccat gcatcataat
    21901 atccaaattt aaattttttt aatatagagt ttacctctag tctacactct tgaataactc
    21961 tttcctctat ccaaattctt ataaattcat cccatttatt actatctatt tattactatc
    22021 taaatttttt agtatgtttg taattagaaa tggatttatt cctttatgtt ttttataaat
    22081 agtttataac ttaatttatc atcaaacaaa tcattcatac aggattcctc ttttttttag
    22141 atattcaatt cttactttta ataaatattt aatacatcct atcttatgtg gttttaaaaa
    22201 gtgtttatag ctatcaaaaa tctctaatat ttcattcata tttattttgt catagtaata
    22261 ttttacatct tttatattga caaaaaagtg ctctaagtgt atagtgtgta tataacaaca
    22321 tcacactgaa aatgataaaa agggggtgat aaaaaatgaa agtgctcatt tctaacacat
    22381 cagataagcc attataccaa caaattaaag agcaaattaa agaagcaata tttactgaag
    22441 aactgaaaga aggggatgca atgccatcca taagaaattt tgctaatgat ttaaaagtta
    22501 gtattttgac tattagacga gtgtatgaag aacttgaaaa tgaaggcttt cttacacgcc
    22561 aagttggaat aggaacattt atttcaactg gaaatttaga aatactaaga gactcaaaga
    22621 gacgtatagt tgaacaaaaa atggcagata tgattaagga tgcaaaagca ctaggaatta
    22681 cacagataga gttacaagaa atgatggata ttctatatca ggaggattaa tatggaaaat
    22741 attttagaag tttataactt aaataagact tataccgatt tttcattatc taatataagc
    22801 tttgcattac cagaagattg tataactgga tttataggtg ttaatggagc aggaaaaaca
    22861 acaacaatcc gttcgatatt ggatgtcgta aagcgtgata gtggaaaaat aaaaatcttt
    22921 ggtaaagatt tcgatggaaa tgagaaggaa ttaaaaaatc gaattggagt tgtatttgat
    22981 gattcatatt tctatgagga gttgtcaatg gcagaaatga aaagtattat tgcaccttca
    23041 tatgataatt ggtgtgaaga agattatcaa caaaatatga agcgttttaa tcttaaccca
    23101 aaacaaaaga ttgatacact ttcaaaagga atgaaaatga aatttgcttt agcattagca
    23161 ttatcgcata aagctgagtt tttaattatg gatgagccta caagtggttt agacccacta
    23221 atccgtagtc agatacttga catactaaca ggatatatga agaaatgtgg tcgtggtgtt
    23281 ttcttttcca ctcatataac atctgattta gacaaaatag cagatatgat tgttatgatt
    23341 gacaatggac gtattatatt agagcgtgaa aaagatactc tattggacga acatagaaaa
    23401 gtaaaagggg atattcaatt tctaaatgat gaaaccaaaa aattattttt aaatttagaa
    23461 attacaggtt atggttttat agggattacc tcaaaaatta atgaagtgca aaaaaatatg
    23521 ccagatattt taatagagcg tcctacaatt gaagatatta tgcttgcata tattgaaagg
    23581 agagagtaat tatgttgatt catttagtta aaaaagatat tctgattgta aaaaaatatg
    23641 tattgttgat attatttatt gcaataggaa tcccattatt tatattatgg cgtttacctc
    23701 catttgcaca aatattggga tttgtactat caattatatt ttcagaattt atgctgtgtc
    23761 agtatctatt attaaaggag agtcaatatt caaaagcatc tgtattactt tgttcaactc
    23821 cttatcctag acgtggctta gttcaatcta agtatgtttt atttgcaatg gtatttatct
    23881 attgtacatt agtttactgg attgaaaatt tacttattcc acaaatagga acttttgact
    23941 tgacatatat tttgattgtt ttattaataa gttctattat ttatggagtt tatatgccta
    24001 ttcaatataa attaggatat gaaacaacaa aattcttttt cgtggtgata atcatggctt
    24061 catcatttgt attaccatta actatagctc acagcaattt tgaaattcca tttatatatc
    24121 agtatccatc catgatagtg aatattgtat tgttcctttt aagtttaatt atactgttag
    24181 cttcaatgtt tatttctatt aaaattttta gtaataagga gcttgtataa ggtaaggggg
    24241 acttttatat gccacatttg ccagattggg ctgttttact ttgtatctta cttccaatag
    24301 tatataaagc ttgtcagata tttaaaagta aaaaatagag tacatattta tattgccatt
    24361 tttattgagt aaagttagat ggtatgaaaa ggttacttga aaataaagtt acttggtgtg
    24421 gcagagtaac atcataatat tatagattat atgtttcttg tgaatcaatt tgtatttttt
    24481 aatagataat atccagtaat tttattttcc attgattatt tttcaattat gagtatgatt
    24541 gtgtgtattg tataccattt tttaagatgt ttttaaaaaa ttatatcgtc aaaatataaa
    24601 tctaaggaat cctataaata aagcttttca attttagaaa actaataaaa agtaggaagt
    24661 taataaaaag aagctgtctc aaaatagaga taagcttctt tttcattgct gaaatgacga
    24721 aaatataatt ggtttaatat aaaaaagagc gctttatgaa ctatttattt tgaacagtag
    24781 atacacttgc actcaattta gttactccta agctttatga actaaaagtt tattttgaag
    24841 cctattttta ttaaaagaga ttttattttt ctaaataatc atctatatac agtattgcgt
    24901 ttcttttatt tattcataat ttattttctt attaaagttt ttctatattc atattttcca
    24961 tattacttta cttttaattt tatacgaatt atcaaaaata aaatagttat tagaaaaaaa
    25021 taaaagtaat tgcaaagctt gcaattactt acttctatat taattactat accataaaat
    25081 aacaaaaaaa tcaagtcgag taatccttca acttaagtga tatactaggt attaataatt
    25141 tttaattaat aaaatataac tagaggaaaa tctacatttt ctcaatttct ttaattaaat
    25201 tgacctctaa ttaagtaatt ataaggagaa aaattatgaa tttagcaaag ggattagatg
    25261 ttttaaaaat ttcatcaaat gtattcggtg aagataaagt tatgtatatt cctgtaatct
    25321 acacagaaga tgatgcaaca cttattgaca caggtcttcc gggtcaaggt gatttaataa
    25381 ttgatgctct taataaaagt aacacgagct ttgataggtt aaaaaatata ataattactc
    25441 atcatgatat agaccatata ggaaacataa attatctaag agaaaaatct aagaataata
    25501 tcaaagtata tgcatacaaa agtgaagtca gctatataac aggagaagaa actcctttta
    25561 aactttatat gttggaacaa atggtagata aaattgatga taagatgctt agtatgctaa
    25621 atgttatggg acttggattt aaatcatcat atactaaagt agatgtgtca ttagataacc
    25681 atgaaaaatt gaatctaggt gaggaaatag aagttataca tacaggaggt cacactagag
    25741 gtcacatatg tctttattta aaagagtcta aagtacttat tgctggagat ttgttacagg
    25801 tagaaaatgg agaacttaag cctgttgatg tgatgtacag caacaaacaa gagttaaaag
    25861 atgctattaa aaatataagt aattataata tagaaacgat agtttttagt catggtggat
    25921 tatatcaaag aaacataatt ggaacattga aaaacctaat tattgagtag tactatatga
    25981 tttacaataa tttcttaaat tacttaaaat tatatctaaa gatgtagaat caaaattaaa
    26041 tataagtaat aagaattttg ctttaaaatt atacttaagt gttaagggtg atatgtagta
    26101 ttacaagtat tttagaatat agttttttat agtaatttta aggttctaca atacttcagt
    26161 tgaaaaatat ttaacagcat agtataatgg gaaagaaaat actttaaata aactttagta
    26221 tacaaagtga agaaaaatat ttaaattata tataatattg tagagtgagg taaaatattt
    26281 aagttatata tcataataca gagtgaagaa aaatatttag ataatgtacc ataatacaaa
    26341 gtgagtaaaa atatttaaat catgtatcat aatgcaaagc gaagaaaaat atttaaataa
    26401 tgtaccataa tgcagagtga gtaaaaatat ttaaataatg tgttataata caaagcgaat
    26461 aagattattt aaagcatgta ttataatata aagtgcgtaa aaatatttaa atcatgtatc
    26521 ataatacaaa gtaaaaaaga ttatttaaac catatatcat aatgcaaagt gaagaaaaat
    26581 gtttaaatta tgtatcataa tataagcaga gtgaagaaaa aaatttaaat aatatatcat
    26641 aatgtagagt gagtaaaaat atttaaatca tgtatcataa tataagcaga gtgaagaaaa
    26701 tatttaaatc atgtatcata atataagcag agtgaagaaa aatatttaaa taatatatca
    26761 taatgtggag tgaggaaaaa tatttaagtt atgtatcata atacaaagta aagaaaatca
    26821 tttaaattat atatcataaa aataatatga aatattgaac actcataata ttagagactt
    26881 taatatttta attgataggt tatattattt ataacattaa aaatacaatt tacataaaat
    26941 agacatatga aaaataaaaa attatataat aattatggga atgaggttct cccttggatg
    27001 ataatatcct aaactgccaa tgagctgatg acttctatga tatcatagat gccaccagct
    27061 ttttttgttg tttaaatcaa taaaatggag gtgaagtact tactaaattt ttatttttta
    27121 aatttgaaga aatatttaat taatagaaaa aatagtagat tatatgttaa ttattttatt
    27181 aagaatgggt taagagtgta cctatatcct atcaatttaa aaaacaatta tgatagtcat
    27241 tacattgact acagttgatg caataaaact ttcaaaaaat cttatgatgc aaatagaaag
    27301 gaattaaaat gatagaaaaa tttaaaactt taatatggat taaaatgata ataagaatta
    27361 aaagcgttta taaaacatat ggtggactat tttttttagg actcataggt ataccagttg
    27421 gtgcaattat aggtcttata gacaccatat ttggaacggt tcttctaaaa gttacagata
    27481 ttcgagaaac atatccaatg tatctgatac catttttagc agtagtagga gttgttattg
    27541 cttattgcta ttttaaattt ggaggaaaaa gtagtaaagg aatgaatcta atatttgagg
    27601 tagggcatgg agaagaagaa attattcctt taagattggt tccatttata atatcaggta
    27661 catggttaac ccatttattt ggtggtagtg caggacgaga aggagttgct gttcagattg
    27721 gtgcaacttt ttcacattgg gtaggtaaaa ggttgcctat aaaaaatgct tctagtattt
    27781 ttctagtaac tggtatggca gctggttttg caggattatt tgagacacca atagcagcaa
    27841 tactatttgc tatggaggtt ttggttgctg gttcactgga gtatcaatcg ctttttcctg
    27901 cgtttactgc ttcttttaca gcaagtgcag tatcaaaagc tttagggtta gagaaatttt
    27961 cttttgcact ttcttctgaa gttgtatttg acttgtcaat attttggaag cttattgttt
    28021 taggaattat ttttggaatg gttggtggag catttgcatg gtgtctgaaa ttgtcaaaaa
    28081 gaaaaatagg gaatagatta aaaaatccta tgataaggat tgctattatt ggagtatgtc
    28141 ttagtgtatt attcctttta ttttataaag gaagatattc aggtttaggg acaaatttaa
    28201 ttcaaaatag tttctatggt ggagaaattt attcatttga ctggctgtta aagtttatac
    28261 ttacaatact gactttgtca gcaggttttc aaggaggaga ggttacacct ttgttttcca
    28321 ttggagctag tcttggtgta ttgttagcag gattttttaa tttgccaatt gaattagttg
    28381 cagcgcttgg ctatgctagt gtctttggta gtgctacaaa tacttttttt gcacctgtat
    28441 ttataggggc agaagttttt gggtacagct atttacctta tttctttgta gtatgcgcta
    28501 tttcatatat atttaatatg gataaatcta tttattcact acaaaaaatt tcaacaaaac
    28561 aataaataaa acaacataat gtatgattag aagagaaaga atattagaga aaggataggg
    28621 taggaatatg ttgacaagtt tagctttgat ttttttatta ggaatggcct cgggaggtat
    28681 ttttaagagg ataaagttac ctaacttatt gggaatgtta cttacaggaa ttatattagg
    28741 tccatatgtt ttaaatttga ttgataattc aatactagat atttcttctg accttagaaa
    28801 gattgcattg attattatat taacaagggc tggattatct ttagatataa acgatttgaa
    28861 gaaagtggga agacctgctg tacttatgtg ctttattcca gctacttttg aaattatcgg
    28921 aatgatagtt ttagcaccaa agttactagg cgtttctata ttggaagcag cagtcatggg
    28981 tgcagtagta ggagcagttt ctcccgcaat tattgtacct aaaatgctta aattaatgga
    29041 agaaggttat ggtacagaaa aaagtattcc acagatgctt ttagcaggaa cttctataga
    29101 cgatattttc gtaattgtga tgtttacagt attcacaggg ttggcacaag gaaatagtat
    29161 atcagctatt agttttttac aaattccagt atcaattata ttaggtgtaa tagctggagc
    29221 agtgattgga atctgtttag cagtattttt taagaaggtt catatgagag attctgctaa
    29281 aggggtgttg cttttaagta tctcattttt aatgataagt ttagagactg ctttagaggg
    29341 tattgtgcca ttttctggat tattagcagt tatgagtatt gggattttcc ttcagataaa
    29401 atatcgtgta gttgcaagaa gattgtctat aaagtattcg aaactatggg ttggtgctga
    29461 aattctcttg tttgttttgg taggggcaac agtagatata agttatgcat ttaaagcagg
    29521 aattggagct gtaattttaa tatttggagt tcttttattc cgtatggtag gagtattctt
    29581 ctgccttatt aagacaaatt tgacaataaa ggaaaggttg ttctgtatga ttggctatat
    29641 tccaaaggct acagtacagg cagcaatagg aggtgtacca cttgcaatgg ggatggcatc
    29701 tggacaactt attttgacgt tagcagttct agctatactt ataactgcac ctcttggagc
    29761 ttttggaatt gatgtaactt ataaaaaatt attaacttct gtaaaaggcg agagtaagta
    29821 aaaataaaga ttattttaac tggtaagtta tattttggaa aaggattata ctattgaata
    29881 caatataaaa aaatcatttg ggagattaag aaggtagaag ttaagaacag attttaaggt
    29941 atgttatgat tattagaatt gattttgtga aattattatt gtaatattta attatataga
    30001 taaagaagcg gagtgataac tatgtgttta aaaaaattat caaaattaga gttagttatt
    30061 atgaaattta tttggaattt agatataaaa accaattcat atgagattat tgattatatg
    30121 aaagaagaac ataatttacc agaaaaagta gctttaaaaa ctttatcaaa actatcaaag
    30181 aaaagattct tatatgttca agaaacaggc aagtgtatgt attatacagt tgcaataaaa
    30241 gaaaaggcat atttagagtt tatatcaaga aatgtgcaaa atcttttgaa gaataatttt
    30301 ataaggaatt tattagtatc atttcacgaa gaggagctaa cagaggaaaa gataaagtcg
    30361 ttagaaaact gggtagtaaa ttgggaagaa gcttatgtat aaatgttatt atttttatta
    30421 caatttagat aattttggtt ttgtaagctc tgcaactatt tttagatggt tgcagagttt
    30481 atttttattt tttattccct taagcaatac ttaaaagtaa acgcatttat atttactaac
    30541 ttcaattact tctaatactt aaattacttt ttaaaaatat tttgatatgt catctctata
    30601 gtaatctaga acttgtttct attctttata taaa
//
LOCUS       DAMJYC010000045        30374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJYC010000045 DAMJYC010000000
VERSION     DAMJYC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.046"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            100..1164
                     /locus_tag="PC472_002136"
     CDS             100..1164
                     /locus_tag="PC472_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2727461.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1322..2008
                     /locus_tag="PC472_002137"
     CDS             1322..2008
                     /locus_tag="PC472_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727462.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            1998..3029
                     /locus_tag="PC472_002138"
     CDS             1998..3029
                     /locus_tag="PC472_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727463.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3111..3776
                     /locus_tag="PC472_002139"
     CDS             3111..3776
                     /locus_tag="PC472_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727464.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3789..6206
                     /locus_tag="PC472_002140"
     CDS             3789..6206
                     /locus_tag="PC472_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727465.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLEKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6387..7178)
                     /locus_tag="PC472_002141"
     CDS             complement(6387..7178)
                     /locus_tag="PC472_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2727466.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7211..7798)
                     /locus_tag="PC472_002142"
     CDS             complement(7211..7798)
                     /locus_tag="PC472_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2727467.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7975..9366)
                     /locus_tag="PC472_002143"
     CDS             complement(7975..9366)
                     /locus_tag="PC472_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2727468.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9556..10854)
                     /locus_tag="PC472_002144"
     CDS             complement(9556..10854)
                     /locus_tag="PC472_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2727469.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIVNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLETLEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11214..12089)
                     /locus_tag="PC472_002145"
     CDS             complement(11214..12089)
                     /locus_tag="PC472_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2727470.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12282..12968)
                     /locus_tag="PC472_002146"
     CDS             complement(12282..12968)
                     /locus_tag="PC472_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2727471.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13017..13550)
                     /locus_tag="PC472_002147"
     CDS             complement(13017..13550)
                     /locus_tag="PC472_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2727472.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13594..14226)
                     /locus_tag="PC472_002148"
     CDS             complement(13594..14226)
                     /locus_tag="PC472_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2727473.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14316..15395)
                     /locus_tag="PC472_002149"
     CDS             complement(14316..15395)
                     /locus_tag="PC472_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2727474.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15528..16874)
                     /gene="pepV"
                     /locus_tag="PC472_002150"
     CDS             complement(15528..16874)
                     /gene="pepV"
                     /locus_tag="PC472_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2727475.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16943..18223)
                     /locus_tag="PC472_002151"
     CDS             complement(16943..18223)
                     /locus_tag="PC472_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2727476.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18350..18610)
                     /gene="hfq"
                     /locus_tag="PC472_002152"
     CDS             complement(18350..18610)
                     /gene="hfq"
                     /locus_tag="PC472_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2727477.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18724..19659)
                     /gene="miaA"
                     /locus_tag="PC472_002153"
     CDS             complement(18724..19659)
                     /gene="miaA"
                     /locus_tag="PC472_002153"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2727478.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19697..21664)
                     /gene="mutL"
                     /locus_tag="PC472_002154"
     CDS             complement(19697..21664)
                     /gene="mutL"
                     /locus_tag="PC472_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2727479.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21675..24518)
                     /gene="mutS"
                     /locus_tag="PC472_002155"
     CDS             complement(21675..24518)
                     /gene="mutS"
                     /locus_tag="PC472_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2727480.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(24678..25412)
                     /locus_tag="PC472_002156"
     CDS             complement(24678..25412)
                     /locus_tag="PC472_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727481.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25381..26394)
                     /locus_tag="PC472_002157"
     CDS             complement(25381..26394)
                     /locus_tag="PC472_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2727482.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSLSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26416..27186)
                     /locus_tag="PC472_002158"
     CDS             complement(26416..27186)
                     /locus_tag="PC472_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727483.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27161..27451)
                     /locus_tag="PC472_002159"
     CDS             complement(27161..27451)
                     /locus_tag="PC472_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2727484.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27582..27681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      28301..28390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28443..28619
                     /locus_tag="PC472_002160"
     CDS             28443..28619
                     /locus_tag="PC472_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727485.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(28734..29090)
                     /locus_tag="PC472_002161"
     CDS             complement(28734..29090)
                     /locus_tag="PC472_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727486.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            29557..30305
                     /locus_tag="PC472_002162"
                     /pseudo
     CDS             29557..30305
                     /locus_tag="PC472_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT        10313 a         4812 c         3500 g        11749 t
ORIGIN      
        1 ttacgcttaa attgaaaata taactttatt cttatataat taaattatac aacaataaag
       61 ttatattttt taaatatatt tttactaggg ggaatagaat tgaaaagggt tgatgagaga
      121 aatactatgt ttgcaagagc aaattacaaa aaagatagta ctgcttataa tgactattat
      181 aagaaaaatc ctgacaaaaa agaaattgat gatagtataa gaaatagacc aaatttatgt
      241 agtgaaggaa caatgactta caatgagtta aactctccaa tggcaagtag tgcattcgat
      301 tttttatctg atatcaaatc tttatgcgaa ggaaaagttt ctgatactaa agtagatgta
      361 gatgcaaagt caatgacaaa aaaaattaag ggtcttgcaa agcagtatgg agcttctgta
      421 gttggtataa ctaaattaaa agactatcat atttatacaa ataggggtag acatgaagaa
      481 aattacggtg aagaaattaa tctaactcat aagtatgcaa ttgtatttgg atgtgagatg
      541 gataaagaaa tgataaatag agctccaatg atatgtgaag ttatagaaac atcaaagtgc
      601 tatgtggatg catctatcgt cggcatgatt ttaagttact atataagaaa tttaggatat
      661 gatgctagaa atcatatgga tgctaattat ttagtaatgc ctgtttttat agctcgtgat
      721 gctggtttag gagatatagg aagaaatgct atacttacaa ataaagatta tggctctaga
      781 cttagattag gtgttgttac tacagatatt cctcttttag aagatgaata tgttgatttt
      841 ggactagaag atttttgtaa ggtctgtaaa aaatgctctt ttaactgccc ttctcattcg
      901 ctttctaatg atattaaaat tggtgatgat ggaaaatata attgggtaat tgaacatgag
      961 acatgctata ttaaatggag atatttaggt acagattgtg gtatgtgtat atctgcttgc
     1021 ccatttagcc aaaatctcga aactataaag aatactactt catttaaggg taataatgag
     1081 cttattcaaa aagctttaga tgagtatact tcaaaatttg gtaaaagaat atttatacct
     1141 ggaaatccat cttggttgaa ataattttat ttaataaaga atctgtacta gaatttatca
     1201 taggacaggt tctttattaa atatataaat ttttaaatac tttttctcgt ttataacaat
     1261 tcagcaatgt ttttagtcta taattgacat tataaaatat taaactaaaa ggagtaactg
     1321 tatggaaaaa atattagtag tagaagatga ttctatatta aataaaactc tatcatataa
     1381 cctaattgaa gatggatata taattacttc aaaatttaca gctaaatctg cactaaagtc
     1441 tatttttgaa tgtgaatttg atttaataat tttggatatt aatttaccag ataaaagtgg
     1501 gtttgaatta tgtaatgaaa taaaggaaaa ttacaatata ccaataattt ttttaactgc
     1561 taatgatatg gaatgtgata tgattaaagg ctatgaactt ggagctttgg attatataac
     1621 aaaaccattt aatatcaata tatttaaaca aaaaattaaa gcttttttaa atcatcttac
     1681 aataaaaaca aaacaagatt actataggga tggatatctt gaaattaact tttctgagtt
     1741 atctgccaat ataaatggaa atcaaattat atttacacca ttagaatatc gtactttaaa
     1801 actgcttact gaaaatccta aaagtatttt aacaaggaaa gtacttttag aaaaactctg
     1861 ggatatagat gctaactttg tagatgaaca tactttaaca tctgttataa gtaggattag
     1921 aagcaaaatt gaaaaagaca atcttaagta tatcaaaacg gtatatggta tgggatatat
     1981 gtggttaggt gaaagaaatg aattttaaaa aattttcttt tagttctatt tatttaataa
     2041 ttgctgttgg tataattttt acagctattt tatttagtat ctcaatttat ctaataactg
     2101 atgatttaaa actaattgct ctattattaa tatatacaat agtactttgt ggatttatag
     2161 cattaataat atttttactt cataaaaaaa tagttatatt ttgttctaaa atatgtcaca
     2221 ctttagatag tatgatgaat agtgaagata atattgaaat tgaattagaa gaagaaacta
     2281 ttttatcacg tatcaatcac agattaatta gaatgtatga agttattcaa gaaaatcgca
     2341 atagtattgc tatagagaaa gctgatttac aagaactagt ttctgatata tctcatcaaa
     2401 ttaaaactcc tattgcaaat ttaaaaatga ttaatactac tctactaaat caaacattta
     2461 aaacagaaat tcaaaaggat tttttaatgg atatggaaaa ccaattagat aaattagatt
     2521 ttttaatgca atctatggta aaaacctcca gactagagac aggtataatc acactatcta
     2581 aaaagaaaaa ttcaatttat gaaacaattg ctacagcact tagtggaatt ttgtttgatg
     2641 ctgaaaaaaa gaatattgaa gttacagttg attgtgaccc aaatttatat ataattcatg
     2701 acaagaaatg gacttctgaa gctattttta atatattaga taatgctgta aaatacacaa
     2761 gttctaatgg taagatacgt gtagctacag aatgttgggt tatgtataca aaaatagata
     2821 ttattgataa tggaaaagga atatcagaaa gtcgccaagc agaaatcttt aaaagatttt
     2881 atcgtgaaga agatgtccat gacatagaag gtattggaat aggtctctat ctttcaagaa
     2941 aaattatatc tcttcaaggt ggctatatta aagttacatc agatattgga aaagggtcga
     3001 ctttttctat tttcttacct aataaataaa tttttaaata tttcatttgt aatgattctg
     3061 caacattttg gttttatact gtatttacaa gatgttaagg aggtattatt atgagtattt
     3121 tacaagtaat taatttaaag aaatattatg gacaaagttc aaacattaca aaagctcttg
     3181 atggtgtaac tctatctatt gaaaaaggcg aatttatttc tattgtaggt acatctggta
     3241 gtggcaaatc cacattatta aatatgatgg gaggattaga taatcctaca tctggaaagg
     3301 ttattataaa gggtaaggaa ttatctacta tgactgatga acagcttaca atctttagac
     3361 gcagaaatat aggttttata tttcaaaatt ataatcttgt tcctttactt aatgtatatg
     3421 aaaatattat cttaccaata ggattggatg gagagaccat agataaggat tttataaatg
     3481 aaattataaa tatgctttct cttgaagaaa aattatatga tatgccaagt aatctttctg
     3541 gtggtcaaca gcaacgtgtt gctatagcta gagcacttgt aacaaaacca gctattatac
     3601 tagctgatga accaacaggt aaccttgata gtagaactag ttcagatgta ctgagtttac
     3661 ttaaagtgac aagtaaaaaa ttcaaccaaa caattgtaat gattacacac aataatgaaa
     3721 ttgcacaact aactgataga attattcgta ttgaagatgg aaaaattgtt aactaggagg
     3781 tgtagtatat gtttagaaat aataatcgca catttataag aaaaatagct ttaaatgatt
     3841 taaaaattaa taaacttaaa acatatcttt caggtatcat tataatgatt tctacatgtt
     3901 tattattaac agtgacctta gtttcatata atgcctctgt agatttagtt aatgcatctc
     3961 catatcatgc tatatataaa tctgtagatg aaaaagcaaa aaatatacta tataaagata
     4021 aaaactttag tggcgtagga acttacaaat taattggaag taataaaaag acagattgta
     4081 ttatgtcgat tgtttatgca gatgatacag caattaaact tatgaatttt caaccattga
     4141 aaggcaaact acctacaaat aaaaatgaaa ttgctatttc agaaaaatat ttacaggaat
     4201 ttggtttaga taaagatatt ggagatagca ttaaattaaa ttattatgat gatattacga
     4261 ataaagaagt tcaatgtgat tttattattg ttggatttct agaaaattat tatcaagata
     4321 atgccaagca atactataca gtcgtttcaa atgattattt taagaatata gcttctacac
     4381 ctttacaaaa taaaagtaat acttttgacg agtcaagacc tgatactgta gatgtattag
     4441 ttaaactatc agaagaaaat acagagaaag attcagcttc aattaagaca cagttaaaga
     4501 aaattggttt atctttaggt attaagaaat ataatatata cttaaatgat aactatattg
     4561 aatcaaattt aattgatggt gagcagatta ttacagtttt agtagttgga atagttgttc
     4621 ttttttctag cgtttttgtt atatatagta ttttttatat ttcagttgtt aattcggttc
     4681 agatgtatgc taagttgaaa tctttaggta tgacttcttt tcagttagaa aagattattt
     4741 ctctacaagg aaatattttg tcaattattt ttattccatt aggggttata gcttcttgta
     4801 taattgctta cataattcaa ccattagcat ggcaaatgat agctgattta tttataattt
     4861 taattttatc actggtaatg tttttaactg ttagaatttc tttatttaaa ccagctagaa
     4921 ttatttctaa aatatctgct attgaagcca tgcaatatac agaaactaaa tttagaaaaa
     4981 aagcaaaaaa ttttagttat ataaatataa aaaatctagc attaaagaat atcgagtcaa
     5041 atagaaagaa aaatttgatt gcattaatat cgctaagcat tagtggagtt ttatttattt
     5101 caatcgctaa tcttgctaat tcaatagatt tcaaaaaaca actatctcaa caatttgtct
     5161 ataacgaaga ttatataatt agcattaaga gtgataactt atatgagcat attacagaaa
     5221 tccaacaaaa taatccacta acagattctc ttaagaatga aattaaatct attcatggag
     5281 taaaaaaatt aattgaatcc aaatcaatta aaagtaatat tatagaacct tacatcaaag
     5341 atagagacga aaaacaattt attactctaa ttaaaggtat tacgcctgaa ctttcaaaat
     5401 cacttgaaga atatattgtc agtggaaaaa tagactatac acatttagat tcagatagtt
     5461 taattataaa taaatataga acaaaatatt atggattaaa tttaaaagtt ggagatacag
     5521 ttacatttaa tatctttagt ggaaatgata ttattaaaaa gaaaatgaaa attataggaa
     5581 tcattgacaa ttccaataca ggaggtatgt tttttgcttc agacaaaact ttagaatctc
     5641 taacaccata taatacaaat ttagattttt ctattttaat agataaaaag cattctaaag
     5701 aagttgaaga aaagttgtta agtataattc agaaaaattc aaatttaatg ctttattcat
     5761 atgaagatga ttatagaatg attactagag catttcaata tataattata gcttcataca
     5821 tttttgttgg tttaatttct tgctttggta tactaaatat gaccaatacc ctaataaaca
     5881 gcgttttaat tagaaaaaaa gagtttgcat tacttcaagc cgttggaatg acaagaaagc
     5941 aactgcgaaa tatgctttat agagaaggtc taaacataag tatcaaagct atatgtactt
     6001 caagtatact aggttatttt ggttcaaatc tactttgcac ttttataaaa gatgtaatta
     6061 gattagactt tattaatttt aaattttcta tatttactat tttaattttt tcattcgtat
     6121 taattggtat acaagtttta gtgactgaat tacttgtaag aaatatagaa aaaaaatcac
     6181 ttacagaacg cttacgttct cagtaagaat gtatataaaa aatagccatt ttttatacaa
     6241 gttttaacat aattttttaa aagctaaagg gatagaaaat tttgacttta cataattttc
     6301 tatccctata cttgttttta ttttatacag atacacttac ttatatctcc gagcaacatc
     6361 accagagaca taaacaagca tattccttat tttctaaata tgtcaattgc atgacttgta
     6421 acccctacta aatcttcacg ttcacaagta gcaaggtgat attttaaata taatgcaaat
     6481 gcatcatcat ccatagagtc tactgcttca cgcatataca aagccaaacc atctgatgcg
     6541 acataatgca gtctagttac attaaatgca gacatcaaat catcaatgtt ttctttacgt
     6601 acaagctcaa acaaatcctt tggttcagac ttagcagcaa atgtctgtgg atcaatcaaa
     6661 cctttttcaa tatattcaga tacattaata tttcctctat ggaacccctc gtcaatgaga
     6721 cagccatcag atataacata tgcagcaaaa accacaccac caggttttgt tacacgaatt
     6781 gcctcatgta aagcttgttg tttatcttct ttgttataaa gatgatatag tggtcctaac
     6841 agtaacgtaa tatcatattt attatctggg aaaacagata aatccatagc attaccctga
     6901 gtaatgctta tattttcatt tgattgagta tttttatgga aaacctctat attatgttcg
     6961 actaattcca ctgcgtcaac atcatatccc tgacgtgcca gtgtgtgaga ataacgacca
     7021 gttgcagcgc caatttcaag cacacaatca cctgatttta tgtacttatc aatgtaatgc
     7081 atagtagtga gaaattccac cattccatgt tttaatgcaa gtcgactatc ttcatcataa
     7141 gtattataaa agtcaattaa gtattgtttt gtttccataa tttcacctct tctatattag
     7201 ttaataaaca tcaaatagac aaattccaat ctggtttttc ttgtcttata aaagctgtta
     7261 atatattttt caacattttt tcagcactat atctatctaa tgttaatttt tttgttgctg
     7321 ttaagacagc tatgccatga gtataaagaa ataaatctaa aaatattact tttgcacatt
     7381 ccaactctat tccaatgctt tccgaaaaaa gtcttgcctt tttttcattg tcaatttctt
     7441 tataaaaatc attcgcctct ttcatcatca aatctaaatc atttataaat aacaacttaa
     7501 ataaatacgt ttcttcttga gcaaattcaa tgtatgaaag cggaagcagt aaataagggc
     7561 ttacactttt agaattacga taattaacaa catactgatt ataatattca tatgcaaaag
     7621 caagaaattc attttttaat tcattcatgt tttcgtaaca agtaaaaatt ggtcgagtgg
     7681 aacactgtaa tttacttgca atactcctcg catttacagc ttcaaatccc acttctcttg
     7741 taatatccaa gactgtattc aaaatcattt cttttgttat ctttacttta ggtggcatat
     7801 atgttcacct ctccattaaa tttttataaa acacatacgt tatataacta tagtaacata
     7861 tcaagaaatt atgtcaagaa aaagctttgc atttttttgc aattcctaaa acttataaat
     7921 aaaacatgag ttgcaattaa tctattaata gataacagca actcatattt tcctttaaat
     7981 actcttcttt ttgtctaata tttcatatac acaatcttct attgaattaa tatctttttc
     8041 ttttcttttt ttagaatcaa ttaaaatgaa atacagtata aaacctacag caagtcccca
     8101 tgttccgcct ttagcagcaa ttacacctcc cataagtcca atcaatccct tctcaacacc
     8161 tgtattacat agctccattg atagctttgt acataaatat ccttgaacta aaagtgttac
     8221 tgacaatgct aatggtataa gaggtgtagc aatctgcgca attggataaa aacagataaa
     8281 aattgctgaa acccatacta atgtacccat gccactaaaa acagactgca tggcttcctt
     8341 tccaaccttg tacctttgag caacacttcc tgtaagtgtc gcagctaatg gaccgcacat
     8401 tggtatatat ggagaaaaga ttgccataaa aatatttctt atcccactta taacattaga
     8461 cctatttgaa ttgaaatcta tatattcatc ttttctattt tcactttcag aaagtactaa
     8521 gttttcacct gttataaaat caccaaaggc aattatgtat atcataaaag ccattggtat
     8581 cccttgtata aataacatta cacttggaaa acctattcca aaaggagata ctgtatttat
     8641 cagattttta aaatctacta tgtaaactaa attgtcaaaa ctaaatctag gaatttctat
     8701 ttctctacta aatataccta ctattaaact cactaaaata gctgatacta caccatattc
     8761 tcctaaatga aataaaaatt tattcttaac ttttagagta tcaaatcttt cagaaaacat
     8821 tacaaaacaa gcaaccaaaa ttcctactgt tatagatata ggataagaat caaatctccc
     8881 tttaggatta aactctgata ttatagtcga tataccgaca cccattagta ttccaccttt
     8941 aatagaattt ggtatcttac taactaactt tccagctaca ccagttatac ccattaaaat
     9001 aaatattatt cctaaatcta tttgtaaagc tgttaatgct tgtattcttt caatgcccat
     9061 gctgaacttt aacaaatatg cagttgtaag agttatagaa gctgtaatcc agccaggtac
     9121 aactgggtct cccataaaaa catgcaaaca gtataatatt gagttaatga tagccatact
     9181 ccatgccact tcatagggta atcccaaaat ttgtgtacta aggccaattg tgcctaaact
     9241 tgtcatactt gatattatac cttgaatagc ttctggtttc tcaaatctgt aatgtataaa
     9301 tggaattctt atcttaaaca ttccaaattt tatatgaggt tgttcccttc tagcgagtct
     9361 ttccataact tcactccacc tttcaataac tcttataagt ttacaacttt ataattgttt
     9421 ataaattgtc aatgtcatta aaattagtat ataacatgca aattatataa acaataaatt
     9481 aaatctataa aattgataga ttttgtgcat atatagaaag accgcttcat ttttagttac
     9541 ttaaatcact taatattagt tatttaaatc acttgataaa ggttttttat aaaattcaat
     9601 agttctattc catgattcaa actgtccttt tgaatttcct tctacagttc cacctgaaga
     9661 aaaaatccct ccactcatac tcatacataa agtctccttt agaggcataa tatatggaat
     9721 tggtaacgga tgacccatat tattgtatgt taataattct acgttatatg gataattatt
     9781 ttcttcaaag atttctaatg tctctaaaca agcttcataa ctattccata tgttatcttc
     9841 ttttccagca atcataagta tatctgcttt ggaattttct atttttattc tagcctcttt
     9901 tctattctct gctcttttaa gacttttctt ataagtacta ttaaatccaa atggaatacc
     9961 ttttgatata ttttttttct gttcttcaaa gaatatatca ttgtctactt tgatatatgg
    10021 aaatggctct cctttatatg accatgatga agtatcattt ccactcatca atccattaag
    10081 agcttggaaa caataaatgt gaggttgtga tactacaacc tttttaattt gttcatatct
    10141 tgaagcaagt aaaaaggcca attctccacc ctttgatgtc ccatgtacaa atatttcttt
    10201 agtactggta atttcatttt cactaatcca ctcaaatact ttttcaaaat attctaatgg
    10261 aacctcttct aatttatctg gtaacccttc taaagcaaag tatgcaactg ttagtacatt
    10321 gaatcctcta gatgcaagtg gagctgctaa aaggtctaga gcgtctaaat ttccatcaga
    10381 accacctagc attaaaattg ttttattatt agaattttgt ttataaaata attttcctat
    10441 aaactcatca gatatgtatt ttgtggtaat atcttctgat ttaaatattc tctttaattc
    10501 aacttctttt ctttcaatag aagtttcaaa tatcatattc acaattattg gtttatcaat
    10561 agatatattt tctgcaaaat ttttaccttc agttttagat ttttccagcg aataaattaa
    10621 ccccatacaa ttatcagcct tataagtacc ttctataggt tcaactaaat ctaaatctac
    10681 ttctcctttt tcatttgaac aaaacactcc ataagatgaa aattcttctc ccttgcacca
    10741 tggaaattcc attttcacac ttattttaag tttagtattt ggaattaagt ccgaaaccat
    10801 aatcttaagt ctttcatcca caaatgattc tacattgtat acatttactt tcataatcta
    10861 tttctcctta taataattat ttatgtttta tataaaacag ctttatctaa tgcaacataa
    10921 ataaattttt aaattaagac accatattga ctatttcatt ttatcaacat attccatctt
    10981 tgtcaatata ttgtcttttt tagtaattta aaattatgta gtctatatga aatccagttg
    11041 atttttggct aattaaagat aggaaatgct attaggttta aaatatatct attcaaaata
    11101 aaaaaccact cattttaaga atggttttta atataaacta tttgttatta ataaggatat
    11161 ttttttattt aaatttatat aatcgaacat ctaatattta atgtttaata aaattatgca
    11221 tttgcaaaag tatatatggc attaactctt gctacatata aattctcatc actacatcca
    11281 atatatctag catatttttc aatcttttct tttgcatttt caacatcatt ttcatcgctc
    11341 tccattaact catttatata aatatccatt aatgacgctt tatattctct tatatcatag
    11401 cctaaaaatt cagtcaatct ctcaatttcc ttactaaaat ctccatcttc ttttaccaaa
    11461 aattcgtata catcttccct atatggattt aaagttatta tttgttttac tactttgcat
    11521 tcgtcagcct tcccaataaa accatcttta taattattaa ataaggcatt agattttttt
    11581 atagattctt catcaatata agaatgtacc gcagttatac gattttctat aagggcatct
    11641 acaacagcat aatgaatttt aaaacaatca ttgtataaat aattgctcat tttctttatt
    11701 gtcgattcta tttctaagaa gctcgcgtgg ccattttttt tatttttatt tggtataata
    11761 aaagttttat tcaaattatt aaaaagtctc ttaaaatcta aatatttttc acaataaaca
    11821 cttttaaagg tatcgatatc tatagttata attccaaaac ttactatagt ctcaattcct
    11881 ttcttaaaag actcttctat atatttttct cctaaactta aacctctatc agatatttgc
    11941 tttagatttt gaaaactatt taaacaatct ctttcaaagt ttttagatat ttctaaagca
    12001 tataatttaa agattttcct tatcttatta taattatttt ttgtatcatc aaatctgata
    12061 atagctccat ttacatcaaa agtaatcatt gagttttaac ctccccatta gtttcatcaa
    12121 taaaataaat ttaatataaa ctttataatc ataatattaa acttatttat actataatta
    12181 ttaatttgat gataaaagct attttccttc taaataaaaa atttgtctaa agtttctaaa
    12241 aaatttagac aaatcttagg ttattactct ttaaattatt tttaaattat tccaaatacg
    12301 ccaaataata ttggtataaa tatagctggt aacaaattgg ctacttttat attcgttaat
    12361 ttaagtacat ttagaccaag tcctataatt aataaacttc cagctgctgt catttctgtg
    12421 actacaggat cacttaaaaa tccatttaaa aatgatgctc ccacacatat aataccttca
    12481 tacaaaaata cagatattgc agaaaacatt acacctatac ctaaagaagc agtaaatata
    12541 attgaagcta ttccatctat aacagatttt acaaataaag tatcatgact tcctgtaaga
    12601 ccactttcta atgaaccaac tactgccatg gctcctatac agaataataa actagatgat
    12661 ataaaaccct ctgctataga atctttttta tttcctttag aaaatttact ttgtaaaaaa
    12721 gcacccaact tatttaccca tttgtcaata tctattaatt ctcctatcag agcacctaca
    12781 gctacggata ttattgtcac taatgtgttt tttccttcta gtgctcctga tataccaatg
    12841 tacaatacac ataaagcaat tccattcatt atagtatcgc ttactctttg aggtaatcct
    12901 ccttttatta taagacctac tatacatcca cctgcaattg ctagactatt tgctattact
    12961 ccaaacattt tttctcccct cgattagcta attgaaattt atcctattat taaaacctaa
    13021 acatctatac tttgtattgg atattttaaa tattccaaaa attcttcaaa accttttcta
    13081 gatatattta cagttttagt atttatgtta ggatgaaaat ttaagtaatc ttctttaatt
    13141 acatctttat ctatgtaaag ctcaactttt ctttctgtat catttattaa tgaaaaagga
    13201 tttacacttc caggaagaag tttaagtaca ttgtatagtc tttcaggtga agcaaacgat
    13261 aatctagaac ttcctatctt atctttaagt gagtttaaat caacttgttt ttctccttta
    13321 actacaataa gataatactt atttcctttg gcatttctta aaaataaatt tttacattga
    13381 cacccctttg ttattccatc taaaatgact aattgctctg ctgtatatac agcttcatgc
    13441 tctactacat catattcaac atttaattta tccaaaatat catatatttt agtttcattc
    13501 atgctagatt cattttcatc ttgttctttt ttattattta aatcactcat acttttctcc
    13561 ttaaacactt catcataata tcattgtgag gtattattct actatagcta ttgctcttac
    13621 tggagctcca tcagcatcat tatacttaaa tggtgctgct ataaatagaa atttttctgg
    13681 aacattttct aaatttgtaa gattttctat gattattttt cctttttcaa ataaaatatg
    13741 atgcacttca aatactgatg tgtcatatct atccactgat agcatatcta ttcctatgcc
    13801 ttttatatta gtattagcta tatagtttgc agcttctttt gttaatgtag gataacctac
    13861 atagtactgc tttttatccc aaaattttga ccaacctgat ttaaatatta taaaatcact
    13921 attttttatt tcatcttcat atttttttag atattctaat tctatattct catttccctt
    13981 ttcaagttcc aatacataag ctttcccaac aaaattttct atatccaact tatctagggt
    14041 ctcaccttta gtatacatat gtcttggtga atccatatga gttccattgt gagaaaatac
    14101 actaattaat gtttcttgat aaccattttc ttcaataata gctactttct tgatatctgg
    14161 tctattaggc tctgcatata caggcatgtc attatgtgtc acatgagtta aatcaaaaac
    14221 cttcatcttt atataccccc tatgttttta ttttctatta tatacctaaa ttcattttat
    14281 ttctatcaga ttttattcca ttatatacca tactactata tattttttat aaacctcaag
    14341 ggatttaccg aaaaatcttg gaaagaatta tcagtaaata taacatcttc acccaaaaaa
    14401 cgtttatacc ttccttcttc tttctttaaa gtaattctct gtttttgtga atgattatta
    14461 aaaaccatta tcttaagagg aggttttttt ctattagtct gcactctttt taacatgtat
    14521 tttatgtgca tatcagcttc aggaaatgaa tatccacaaa atattaaaag gtcagtatct
    14581 cttaaagttt tttctgtttc attccaaaca ttacttaaaa atatgtttga catattctta
    14641 aaatatgttg gtggcactat aataggttct gttaactcac cacactctaa acattttgtc
    14701 tctgaactat tttcaataag cttcatgaca ccaccttcat gtggtgtaag agttacacta
    14761 ttacaaatgg ggcaataaag ccaattcaat gacccatgta tcttatataa tttgattaat
    14821 ggttcttttg gttttctcca atttaactcc tctttaaaat ttgtaaactc cacaccataa
    14881 tcaagcataa ttggcaaatc attcttatct cttaatccag taactgtatt gtctatatgt
    14941 atatcataat ttgcactaat aaaagtagta tcaagaagca aatcattttc caaaagattt
    15001 tcaactaaaa gtttatgata tttattatct gttctaggtg tattatgaat tgcttcagcc
    15061 attagcagta ttaaatattg tcttaaaaaa cgtatactat cacttctgtt atttagattt
    15121 tctagtccaa aatttctaaa tgcttctttt ctttgttctg ctaagtctaa aagacctaaa
    15181 acttcttcaa aggtaggaaa atttgcatta tctatattat cttttactat atcaatattg
    15241 aacatctgtt taaagaaaat atacaattct ctattcattt tggtattata atttgtggga
    15301 tttagttttt taaaatattc gctaaaaagt tcattttgaa taggtagatt ctctgctgct
    15361 gaagctcctg ctcctaaaaa tattgctatt ttcatatagt tcctcctaat ataaatatta
    15421 ctcctatttt tatatccatt aaatatccat atcatttatt ttatgataca acaataaagc
    15481 tgtcttttac tagaaaaaat cctattacaa gacagcctta tattatatta aatctttaat
    15541 aattcgtaaa tagaaagagc atatatttga gtagctttta taatatcctc tactattaag
    15601 tattcattag cttggtgttc tgtttctggt tttcctggaa aaatagctcc aaatgcaaca
    15661 caattatcta aagctcttgc gtatgttgct cctccagatg aaagtggtgt tccatcaaga
    15721 ccagtttctt cttcatatac ttttctaagt gtcttaacaa gaagagtatc ttctggaaca
    15781 tagattgaat ccaagaaatc atattcttca taatttaaat tgtatttatc tgtcattttc
    15841 tttaactctt ttacaaaatc atcctttttg tatgttactg gaatccttac atctattcct
    15901 gcaaattctt tttcgttatc aatagttact ctacctatat ttactgttag tttacctgat
    15961 acatcatcct tgcagttagg aattatattg tttccattag catcttcacc aataacttct
    16021 gctaaaaact taattatatt agaatctata cctatattat taagagctat acaaagtcta
    16081 gccactgcat ttataccagt atcacttgct gctgaatgga cactctttcc aattatacag
    16141 attttattac cttcaacagt atactcaaaa tttaatttat taagttcttt ttttaattta
    16201 tcagagtatt ttccaatata gattgcttta cctggaactg catttaaagc ctttccaact
    16261 gacaactcta tatcactttt gctatcacaa gtcaaatgaa cttgtaaaag acctttctct
    16321 gcatttgtta ttgggaatct agaatctgga gtaaatccat agtttggtat ttcttcatta
    16381 ttttctttat atttatttat acatctccaa agattttctt catcagcccc aaaaatgaat
    16441 cttatcctct tattaaagtc tacatttaaa tccataagag ctttcactgc ataaattgct
    16501 gatattgtag gtcctttatc atcttgtgtt cctcttccat acaatttacc atcttctaaa
    16561 acagcttcaa atggagggta attccagctt tctaaatcac cttctggaac cacatcaacg
    16621 tgaccaagta tacctatcat tttttcacct tgtcctattt ctgcatatcc ataatatcca
    16681 tctttgtata ctgtcttaaa tcctaaagat ttacataaat ctaatatacc tcttaaagct
    16741 ttatctacat tttcaccaaa tgggcaattt tcagttgctt cagatataac actaggtatt
    16801 tttacacttt cttgtattga agaaatcatt tcatcttgta gagaatttac tttttcttta
    16861 atttgctctt tcatattatc acccttcaat tttcatcata atatactaat aaatattttg
    16921 accttttaaa aaaaaaatat gcttattttt tcagtttttt tataaattca atcgctttta
    16981 aagtccccat ttttgaatgg tcaaatgtaa gtccaccttg gaaataaaca ttataaggag
    17041 gtcttattgg agcatctgca cttaattcta tagaagaacc ttgtataaat gctcctgctg
    17101 ccattatgac ttcactttcg tatccaggca tgtcccaagg aacaggtttt acataagaat
    17161 cgactggtgc agcttcttgt ataccttcac aaaaacttat tacttcatca gcattattaa
    17221 gtctaataca ttgaataata tcacttctta agtcatcata ttttggtaat acatcatatc
    17281 ctaatttttc aaaagctctt gagcaaaaga tagctcccat tacagcctga gatactatat
    17341 aaggcgccaa gaaaaaaccc tgaagtacat ttctagttgt tccaaaagta agaccacatt
    17401 cttttcctat tccaggagaa gtcattctat aagatataag ctcaatcaaa tcttttctac
    17461 cagctatata tccacctgtc aatgcaagcc caccaccagg attttttatt aaagagccag
    17521 ccataacatc tgctccaaca tcagtaggct ctttggtatc taaaaactca ccataacaat
    17581 tatcaaccat tactatagct tctggtttaa cagactttat tacttctata gcttctttta
    17641 tatcactaat tgaaagtgat tttctccaag aatatccttt tgacctttgt atcataacga
    17701 gtttagttct atcatttatt ttgtttttaa ttccttctaa atctaagttt ccatcctcta
    17761 aaaaatctac atcatcatat gtaacaccat actctttcaa tgaacctttt tcttctctta
    17821 tgcctataac tccctctaaa gtatcatatg gtctaccagt aacagataaa atttcatctc
    17881 ctggtctaac tataccttgt atacataaag taagtgcatg tgtaccattt acaattattg
    17941 gtcttacaag agcatcttct gtattaaata ctttagaata aatttcctct attttttcac
    18001 gaccaatatc attatatcca tatcctgtag tccagttaaa gtgcatatca cttagtttag
    18061 attcttgcat agcttttaaa actttatatt gattatattc tcttatttct tttatttctt
    18121 caaacttatc ttttatttct tccattattt cttgcgatag tttaaaagta tcatcatcta
    18181 ttccatagta gtcttttaat aattcttttg tctcattaag cattttatca cctgtctctt
    18241 tattatttta tttaaacatg taaaggagac tattttacta aaacaaataa attatagttt
    18301 ataagttagt ataagtctcc tctcaatcat cttaaattaa attaattatc tatctgttgt
    18361 tattattatt gttgttttgg ttttgatttg ttagattaat atactttcca ggttgtatag
    18421 ttgatacagc atgtttataa atcatgtttt gttgtctatt atccccttct attaatatta
    18481 tataactatc aaaccccttt acaagccctt taacttgcac tccattaact aaatatatag
    18541 taacaggtat cctttctttc cttgcattat ttaaaaacaa atcttgtaaa tttaaaactg
    18601 tatttttcat tagatttgta ccctcccaag attaaattga acttctaaaa tattcctacc
    18661 ttatattcta tataagttat gacaaatttt caatattaag ttgtatactt aaaaatttat
    18721 atattatttt atagaatctt taatatataa gattatttca ttttttaatt cgtctatatt
    18781 ttcatattgg tctaaatcaa accattttgc tgttttatat cttttaaacc atgttatctg
    18841 acgttttgca taccttctag aatctctttt aattatttca atagcttctt cgtatgtata
    18901 ttctccatcc agatacttta atatctcttt ataacctata ccttgcattg atatcatatc
    18961 ttttttaaat cccatactta aaagtttttt tacctcttca accaatccat ttttaagcat
    19021 aatatcaact ctcatattta ttctttgata caaatgttct ctatctctat ttagaactat
    19081 tattattggt tgatattctt cattaaattt taaatcactt gaaaaatcat tcattttctt
    19141 accacttaga caaacttcaa gagctcttat gaccctttta gtattgtttt tatggattct
    19201 attagcagat tcttcatcaa gttcttttaa cttattatgc atgtaatcta ttcccttttc
    19261 agcaagttgt ttttgaagtt cttctcttaa ttcattattg gcatcagatt gtgcaaaatc
    19321 catattatat attaaagagt ttaaataaag tcctgtgcct ccagttacta aaacacattt
    19381 ttccttttta tttatttcat gtatatattt ttttgctctt tgttgaaact ctgaaactga
    19441 aaatggatag tcaggcttaa cttcatctat aaggtaatgc ttcactcctt gcatctcttc
    19501 ctctgtaact tttgcacttc caatatccat atattcatat atttgcatag aatcagctga
    19561 aattatctct gcattcatat cttttgctaa ttttattgac aaatctgttt ttccaacagc
    19621 agttggacct gtaagtatta taagtggtat ttttttcata acattccttt ccatatattt
    19681 taaatatcca aatattttac ataattcttt taaacatttt ttctatttct gtcttagata
    19741 tttcgaccat tataggtctt ccatgaggac atgtaaatgg attctcacac ttttctagct
    19801 gttccaataa actttttatt tcaatatcgt atattttatc atttgctttt atagcagacc
    19861 tacatgccat agatgcaaat ctctctcctt ttaaatcata attacttgta atttcttcta
    19921 tattatcaat tatttgaagt ataaatttct cggtttcagg tactccaaat atagtaggaa
    19981 cacatcttac cattatatga ttattgccaa aaatttctag ctcaaaacca aatttcataa
    20041 ataactccaa gttattttca atttgaagca tatctacatt tgatacttca ataaccactg
    20101 ggtctaaaag tatttgcata tttatatctt gtctatagaa cttctccata tatctttcat
    20161 atagtattct ttcatgtgca gcatgttggt ccaacaaata catagaatcg tctttgctca
    20221 aaattatata agtattaaat acaactccaa ttacagaata tccgtataaa gaaaacttgc
    20281 gttttgaact acacaagtat tcttcttcac tgtccttaat actatctcct aagtaataat
    20341 ttccttcatt tttaattcca tcaacttgaa actcttcttg tatattatca tttgcactat
    20401 cttcacttag ataattaatg ttttttacat catcttctac acttgcattt aggacttcac
    20461 ttactgattg aaattcattt attggttttt cacttgataa agtaacaact tcaatgactt
    20521 catccttacc tttagtaata ttactatttt taggagtaga ttctaataaa tcattatttc
    20581 ttaatttata atctgtactt ttttctctac tgttaatagc gattctaggt tgtgtttttt
    20641 tatcactata agtagcatat tttccaataa gatttgagtg tattaattta acctttaaaa
    20701 aatctctaag ttctatatac acttcttgtt ctttttcaaa tttaatttca agtttattgg
    20761 ggtgtatatt tacatctata catgatgggt ctacttctat atttaagaag cacactgcat
    20821 gtttacctat tggaattata gacttataac tttctgttat tgcatctatg attattttac
    20881 tttttacaaa tcttttattt atatagatat gttgtaagtt tttatttgaa cggtatatat
    20941 tgttattacc tatatatcca ttcattttaa agtgattgca cttaaactct acatctatta
    21001 tattttccgt tatttctttt ccatatatac ttctaatagt gtttacaagc tttccatccc
    21061 ctggagtatt tagcatctgt ttattgttat ttgtatattt aaattgaatg tttggattac
    21121 ctattgcaag tttatttatc aaatcactta tatttatagt ttctgcatga gtagatttta
    21181 aaaatttctg tcttgctggt gtattaaaga aaatatcttt tataatgatg gtagttccat
    21241 tagtagaacc tattggttct ttcgatatta tttttccacc ttcaacatat attttagttc
    21301 ctattatttc atctttagtt ttagttgtca tttctaattt tgatacagct gaaatagatg
    21361 ccaatgcttc tcctctaaaa ccaagagagt ataaatcata caaatcatca atttttttaa
    21421 ttttactagt agcatgtctc aaaaatgatt tttcaacttc acttgaaggt attcctattc
    21481 cattatctgt aatcttaatt agactttttc ctccatcaat tatatctatg gaaattttat
    21541 ttgcacctgc atcaatggag ttttcaataa gttcttttac tacagaagaa ggtctttcta
    21601 caacttcacc tgctgctatc ttatttatag tcaaatcatc taggatgtta attatatttt
    21661 tcatttttac acctctaaga ttttatttct tttgcttttc tttgaagatt ataaagagag
    21721 tttatagcat ctaatggagt catatttaaa atatccagat ttaaaatttc ttctgatagt
    21781 atatctctat ttacactatc aaaagagatt tgagttaagg ctacttcttt tacagaatcc
    21841 acttgattaa ttttctcttt atcatcattt tgctttatta aagcaacttc atcatttaat
    21901 gctttaaatt tagaatttaa tttatcataa tcctttttta tatgtttgta gtcttttact
    21961 ataagttcat gttctatttt tagagattca tatttattct taaaattact ttgatttacc
    22021 ttaactaatt cttcgtccaa attactatta attatattat tgttttcttt atctccattt
    22081 attgcaacac tgttgtaaac atgatttttt tccaagtctt taagtatata ttttgctctt
    22141 tctatgactt cgtctggtaa tttagctaat ttagcaacat atattccata actcttgtct
    22201 gctccctgag gaattatttt tcttaaaaat attattcctt ccccatcttc tttgactgct
    22261 attgaataat ttttaacctc tttaaactct tcttctaaat cagtaagttc atgataatgt
    22321 gttgcaaata atgtcttaca tctaatattt ttttgaatat attccactat tgaccatgct
    22381 aaacttatac catcataggt actagttcca cgacctattt catctaatat aactaaactt
    22441 ctctctgttg cattttttaa tataagggat acttcgttca tctctaccat aaaagtagat
    22501 tgaccttgag ataaatcgtc actagctcca actcttgtaa atattctatc taatattgga
    22561 atatcagcag actctgctgg tacaaatgag cctatatgtg ccataagagc tattatagca
    22621 gtttgtctca tatatgttga tttacctgac atattaggac cagttattat gttaataatg
    22681 ttttctccac gatttaaata agtatcattt ggaacgaaat tttcctctcc tactatattt
    22741 tcgacaacag gatgtctacc atttcttata tcaagtttat tattttcatt tattgctggt
    22801 tttacatagt tatttatatg tgcaactgtt gcaagggaca caaaaacatc tatattagct
    22861 atagtcttag ccactttttg tattctatct atgtttttat atattgtatc tcttatctct
    22921 acaaatatct cgtattctaa agatttaatc ttttcctctg catgtagtat cttttcttct
    22981 atttccttta attctggagt tatatatctt tctgcattac taagtgtttg ctttcttata
    23041 tatgtctcat ctagctttgc ctgtttaaag ttagcttttg ttatttctat ataatatcca
    23101 aaaactttat taaagcctat ttttaatgat ttaactcctg ttttttctct ttccctgttt
    23161 tctatttcct ttacaagaaa ggctccattc ttagaaatct ctcttagttc cttaagttca
    23221 tcactaaaat ctgatttaat tatgtttcca tcttttatgg ttatcgtagg ttcttctaga
    23281 atagcttcat ctattaaatt gtatatatca tctaatttat ccatttctga aacatactct
    23341 tttaggattt ttgcatttga taaatttata gtatctttta aattaggtaa cttttctatc
    23401 gaatttttta agtgaattaa ctcttttggt gttactctct caaatgctat ctttccacat
    23461 attctttcta tatcgtatat atttttaagt atatcattta ggtcttctct taatataaag
    23521 tcatctttta tttcttctat tacatctaat ctattttcaa ttttagattt atttatcaaa
    23581 ggttcttcta catattttct taaaagtcta cctcccattg ctgtagaagt tttatcaagt
    23641 acgtgtaata atgaaccttt tttcttattc cctcttatag tttgagtaag ttctaaatta
    23701 gttctagtaa acatatctag aaccatatat tctgatgaat tgtatatatt tatattatta
    23761 atgttagatg ttatttgttt ttgagtattg tatatataat tcagaagaat tgataagctt
    23821 gactttatta gacctttatc atcaaacttt agtttttgta gataaacatc tggaaaatat
    23881 tcttttaata tatttatatc caaatagtta tcactaaaac tttcatttat gtatatatta
    23941 cttacagtag ctatatctct aagattttct ataaagtcta aatcatttat aattatttca
    24001 gttggatgga tttttgcaat ttcttcaatt accttatctt catttaaaca tgttgcatta
    24061 gtttctcctg tacttatatc aacataagtt agacctatat tagtaccatc cttatacaaa
    24121 gataataaat aattattctt tttattttca agtaaatttc catctaaaac tgtaccagga
    24181 gtgatgactc ttataacctc tcttctaaca attccttttg ctgtagatgg gtcttccatt
    24241 tgttcaccta ttgctacttt atatccattt tcaactaatt ttgatatata tgaattggct
    24301 gagtggaaag gaacaccaca cataggagca cgttcttcta aaccacaagc ttttcctgtc
    24361 aatgctattt caagagcttt tgaagctact aatgcatctt caaaaaacat ttcataaaaa
    24421 tctccaagtc taaaaaacag tatacagtct ttatatctat tttttacttc aagatactgt
    24481 ttcatcatag gagttaattt attcatatct atttgcatta tcaaacctcc ttcttttcta
    24541 attaaatatt atatcatagt agatgattat tattaacaca atatataaaa ttttgacata
    24601 taactaaaaa caatccaaaa tgtatactca cattttggat tgctaagttt aatgcttatt
    24661 aaataaaaat atttttttta caataaagat attatttctt ttttaagttt aatgatattt
    24721 tcaaggttat cttcatttaa ctttctacta tctataacaa attctttctt tactattgat
    24781 ggtttttttg aaataacata aattctgttt gatagcatta tagcttcgtc aatatcatga
    24841 gttataagaa gtattgttga atttactttt ttcttaacat ctaaaagcca tttttgcatg
    24901 gatgttttag ttaatgaatc aagtgcccca aaaggctcat ctaaaagcat tatgtcatta
    24961 gaatttataa aagttctcaa gaagttagct ctctgtctca ttcctccaga aagttctgaa
    25021 ggatatttat cttcatatcc gcttaatcca aacacctcaa aataaggttt taccattgac
    25081 ctagctttac tctttttttc tttttttaaa attagtggta gtgacacatt atctattata
    25141 gttttatatg gcaagagtaa atctttttga tacatataac tatacttacc atttatattc
    25201 actttgccac tatcataatc tgtaagttta gttataatat taaaaatagt actttttcca
    25261 catccacttg gccctaatat agaaacaaac tcaccttcat aaacatcaat acttatatct
    25321 tttaaaactt caatatcttt ataagttttt gatacatttt ctatagatac ttttactttt
    25381 ttattgtggt aaaaattcat ttgtgtaagc atcacttgcc ttcatatcct tatctattaa
    25441 tttatattct tttaagaatg atgtatagtt gttccataca ctatctttca tttctcccca
    25501 tcgtggtgca tcatctttgt acttgcttgc taaatattct tgactcttta aagctagttt
    25561 ttcatctact tcaggtgcat gttttactaa aatttttgct gattcttctg gattttttat
    25621 tgcatattca taaccttttg ttgtagcttt taagaatttt ttagcaagct ctggattatc
    25681 tttaagcaca gtttcattag aaattataag tggtgtataa taatccaatc tctcatctaa
    25741 atcttttact ggtataaagt ttaagtcata tccaattaaa tcagctttaa ctgcatccca
    25801 accttcaaat atccatgcaa aatctacagt tttcatagct gcaaagaaat catcttgacc
    25861 agtattaact atctttaact tattaaaatc agctccattt ttcttcataa ctgctttaaa
    25921 aacagcttct tctgatagag agccccaacc accataagtt tttccttcaa aatctttagc
    25981 tgttgttata ttcttttctt ttggtgatgc aaacccagaa gtattatgtt gtataacagt
    26041 tgctacagct tttattggaa gtggatcttc actagtcttt gcataagtaa cttgttcttg
    26101 atagcttata ccaaagtcag cttttcctgt tgctactaaa gtttctgctc cactttcagg
    26161 tggttgtact atttcaacat ccaaaccttc ttctttatag taacccttat caagtgctac
    26221 aaataatcca gtatgatttg tatttggtgt ccaatcaagc accatagtta cttttttaga
    26281 agaagtatct ttatcattgt ttttgtcttt attgttattt gaagaacatc cagttattgt
    26341 tgccaatgta aaaacactta ctaaacctaa tattcctaac tttttaaaat tcataattgc
    26401 ctccctcaaa tacctttaat ctattaatct atgtcttata acaacttttt ttacagcctg
    26461 tacaattcct acaaacatta aactaaatat aactactaat atagtagatg caaatacttt
    26521 atcaagtgca tatgcacttt ttgacctaag catataaaca ccaagtcctt tcgttccacc
    26581 tagccattct gcaacagttg ctgccattac agcatatgtt gcagatattt taagtccaga
    26641 aaaaaattta tccattgcca taggaaattt aaggtgaatg aatgtattaa ctttatttga
    26701 gtccatagtc ttaaataaat ttaaataatc tttatcaata ttctcaatgc catctacaaa
    26761 acttattaat ataggaaaaa aacaagtaag cattaccatc aatacttttg gcattgttcc
    26821 aaatccaaac catatcataa agagtggtgc tataattatt gtaggtatca tctgtgtaac
    26881 caacataata ggataaagac atttttttat tattgataca aaatccatca atattccaat
    26941 taccaatgat attataattg cgactataaa accaagtatt gcttctccta tagtaaccac
    27001 agcatgcatg gataaatttt gataatcttt aaaaaacaca ttaattatat ctactggtgt
    27061 gggtagtata tactgaggaa cttctaaaac tcctaccata gattgccaaa gaataagtat
    27121 aaacaaaaat gttgctattg gatatatact atctttgact ttatctatga tttctaagtt
    27181 tttcatctat agtcagacct ccagcttttt catctactct agctactgtt atgactcttt
    27241 cacatccaag attaaaaggg attttatgta ccttttttaa taattcaaaa acttcatcaa
    27301 aatcaccttc aacagtagtg ctcattgctc ctatttcaca tttaagacca cttttttcaa
    27361 tttcagcaat acaagcatct actactttat acatctcttc ttcacctttg taaggtctta
    27421 gtggcattac tgctatgtct gcgattacca tttaaatcac tcctttaaaa ataattaata
    27481 cctactaggt atattttgtt ttttattaaa aaattttatt actaaaaaga tattatattt
    27541 aaataaaaga aaagtcttat ccaaaatagg ataagactta tttttatctt aactggtctc
    27601 cctacactgg cattatccaa acaggttcaa agggtcgaga taaaatctcc tctcagcaca
    27661 ttagctcccc gaaatatatt atatcttttc tacttgatta tactacactt atcatatttt
    27721 aacaaatttt ttatttatat tagatatttt atccgttcaa aaatatctaa tataaattgt
    27781 acattattac accaaataaa ttatcttaaa atgatatttt atcaacatct tccaaaacta
    27841 gattaactga attttctagt ttttattttg ctatttcaag catatttgga tgtatatcaa
    27901 ttccatacaa atattgtgct aaatttctgt tttcactcat ctaatttgca atcgttacag
    27961 attacagatt agaaacagtt tttagactct acttatcttt atctacaaaa aatctttcaa
    28021 taaattcatt aataatattc agtaatctaa aaataaacct ctatctggat ttttaagata
    28081 caaacctttt aggaagtatc ttttgtgata atataaaaat tactcgtcat tatcatcaaa
    28141 atcctcagca accaacattt tatataagaa tcaaactcta taatataatt attttatcca
    28201 atttattttt gagaaataaa atattttttt aatttttaaa ttttttaata tgcaccttga
    28261 aaaaagaaac gttttcctat attatggtaa tagtagaata aaactaaata ggcaaaacta
    28321 gagaaattta gtgacgcaaa gctataggga ctaagactta tgaaatgagt tatgtcagcc
    28381 agttgccaaa gagatatttt ctttttgttt ttatgaatgt tttttagggg gatatatgat
    28441 atatgtttat ggataaaatg gatagatgta cacatatact aactgcatat atatgcagtt
    28501 cacgcgatta ctgcaatttt atagatacac aattaaatga ttttatacta gaatatggag
    28561 agaatgtggt agaatcttgt ttgcatcaag tgatggtatt ggtaagcaga tataattaaa
    28621 gaaattttat tttctagaaa acccttttta gttttatcta cgttttataa ttcattaaag
    28681 aactaattaa aataagcttc attatatatt tttcatgaag taaacagtta agtttatctc
    28741 atatactgtt taccccaatt acataattct aataatatag attccaatga tttaccacgt
    28801 ttagtagaca aatattcaac ttttggagga atagtcggat aagaaatcct cttaataagt
    28861 ccatctcttt caagatactt taattcttgt gacagtatct tatgacttat atgaacaact
    28921 tcttttttta attgactgta acgtattggc tcttgtttta aaatatgcca taaaataatt
    28981 gccctccatt tacttcctat cacagtcatg gtaaaaccaa ttgaacaatt aaaatctttt
    29041 tgtctttctt caatatttgg aaattcttca aaattattat ctatatccat ctcaatcctc
    29101 tctttcaaaa tagtaagtat ctaactaaaa agtgcattct tgtttagcgt actaattcgt
    29161 aatataatct tgattaaaaa atagctatgc acactcgtga ctttagtcat gggaggttca
    29221 gtgtagcata gagccttaca taatactact tttttagatg tatatgttta aattttaata
    29281 tagacatatg ttattttatt aattttattt aaacagataa taataaataa taatttatct
    29341 caagttttct atgtagataa actaatctaa tgtttagaaa ggattatata atatgaataa
    29401 aaaatttcgt ctttatcaag ttgactcatt acaaaagaaa aatttacagg taatccagct
    29461 ggtgtaatta caaatgctga tggcttatct gaatcacaaa tgcaaaaaat agcaagagaa
    29521 ttaaataatt cagaaactgc ttttattttc aataacttgg ctaaaaatca tcaagaaaat
    29581 tatgatgtac atatcagatt ttttacacca actaatgagg ttcctatatg tggtcatgct
    29641 actattgctg cccattatgt tagagcaata gaaaaccaat tcaataacaa aacagtattt
    29701 caaaaaactg gtgctggaat attaccagtt gatattataa aaaaagataa tgattatgaa
    29761 ataattatga cacaaggaaa aattgaagta tctccaccat tacaacaaac ctatcaaaaa
    29821 agattactta atgcatttgg tatttaaagg aagatattcg tacagattgt cctattgcca
    29881 tatcatctac aggacattca aaagttatga ttggaataaa ggaattatca acattaaata
    29941 gtttatctcc aaatttatat gaattatcga aaataagtca agaaataaaa tgtaatggat
    30001 attatatttt tacattacat aataaagaaa ctctacaagt tcatggaaga atgtttgctc
    30061 ctgcaattgg tatttcagaa gaccctgtta caggaaatgc taatggaccc ttaggtgcgt
    30121 accttgtaca ttataaagta atacctacac ccaacacaga gtttatattt gaagcagttc
    30181 aaggagaagc aataaaacgc aaaggcaaga tgttagtaaa agtaattatt ttagataata
    30241 agccagttga agtaaaaata attggaaaag ctgtaattgc attctcaaca aacatagaaa
    30301 tctaattata gattccttct aagtaaattt ttgaactttt taatactaat ttaataacaa
    30361 actaatttat atac
//
LOCUS       DAMJYC010000046        29503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJYC010000046 DAMJYC010000000
VERSION     DAMJYC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.047"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            548..3190
                     /gene="adhE"
                     /locus_tag="PC472_002163"
                     /gene_synonym="adhC"
     CDS             548..3190
                     /gene="adhE"
                     /locus_tag="PC472_002163"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2727487.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3550..3891)
                     /locus_tag="PC472_002164"
     CDS             complement(3550..3891)
                     /locus_tag="PC472_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727488.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3918..4589)
                     /locus_tag="PC472_002165"
     CDS             complement(3918..4589)
                     /locus_tag="PC472_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727489.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4601..7177)
                     /locus_tag="PC472_002166"
                     /pseudo
     CDS             complement(4601..7177)
                     /locus_tag="PC472_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            7385..8320
                     /locus_tag="PC472_002167"
     CDS             7385..8320
                     /locus_tag="PC472_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2727490.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8339..9076
                     /locus_tag="PC472_002168"
     CDS             8339..9076
                     /locus_tag="PC472_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727491.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9261..14318)
                     /locus_tag="PC472_002169"
     CDS             complement(9261..14318)
                     /locus_tag="PC472_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2727492.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIKGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14343..14762)
                     /locus_tag="PC472_002170"
     CDS             complement(14343..14762)
                     /locus_tag="PC472_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727493.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVPDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14995..15246)
                     /locus_tag="PC472_002171"
     CDS             complement(14995..15246)
                     /locus_tag="PC472_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727494.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15335..16066
                     /locus_tag="PC472_002172"
     CDS             15335..16066
                     /locus_tag="PC472_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2727495.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            16125..17036
                     /locus_tag="PC472_002173"
     CDS             16125..17036
                     /locus_tag="PC472_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2727496.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            17087..17899
                     /locus_tag="PC472_002174"
     CDS             17087..17899
                     /locus_tag="PC472_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2727497.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            18008..18700
                     /locus_tag="PC472_002175"
     CDS             18008..18700
                     /locus_tag="PC472_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2727498.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18787..19728
                     /locus_tag="PC472_002176"
     CDS             18787..19728
                     /locus_tag="PC472_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727499.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19765..20922
                     /locus_tag="PC472_002177"
     CDS             19765..20922
                     /locus_tag="PC472_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2727500.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            21074..21634
                     /locus_tag="PC472_002178"
     CDS             21074..21634
                     /locus_tag="PC472_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2727501.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21662..22153
                     /locus_tag="PC472_002179"
     CDS             21662..22153
                     /locus_tag="PC472_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727502.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22382..22933
                     /locus_tag="PC472_002180"
     CDS             22382..22933
                     /locus_tag="PC472_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2727503.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            23019..23951
                     /locus_tag="PC472_002181"
     CDS             23019..23951
                     /locus_tag="PC472_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2727504.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            24006..24869
                     /locus_tag="PC472_002182"
     CDS             24006..24869
                     /locus_tag="PC472_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2727505.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            25116..26978
                     /locus_tag="PC472_002183"
     CDS             25116..26978
                     /locus_tag="PC472_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2727506.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGKRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPIMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26981..28447
                     /locus_tag="PC472_002184"
     CDS             26981..28447
                     /locus_tag="PC472_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2727507.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28471..29307
                     /locus_tag="PC472_002185"
     CDS             28471..29307
                     /locus_tag="PC472_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2727508.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10957 a         3522 c         4696 g        10328 t
ORIGIN      
        1 ggtcttaata taaaaattat ttatctatat gaaaagcata attattacat attgacttaa
       61 aaataactta tagagattag aataatttta gtttatatat tttttaaaac tatacagttg
      121 ttataaaaag aaaaattgca tacaagtcaa aaataagtgt tattttataa aaatacgaca
      181 aaaattttat ttactactta tacaaataac atgcaaataa catataaaat ttaataagta
      241 aaaaaaatga aagatataaa ttatcatttt agtattttat ttagtcgaag taaattattc
      301 aaatgaaata tatctcattt agataaaaaa ttttagtaaa agtaaaaaaa ataatatatt
      361 tgttaaaaaa taaattgaca aaatattcag aaaaaaactt cttgacctaa gagagtaaaa
      421 taaataaaat ctaaggtata gagatgaaat ctataaaatt attttaagta taagttaaaa
      481 atatagatga tgcatttaag atttataaaa attagagatg cataattatt ttagggaggt
      541 ttttagtatg gagaaaaaag aaaaggtagt agaaaaatca aatgttgaag tatgtagtcc
      601 tgaaattgtc aacagtgttg aaacattaag aatgagatta gaagaaatta gattggcaca
      661 aaaagaattt gcaacattta cgcaagaaca ggtagataag attttcttag ctgcttcaac
      721 tgcagctaat caacaaagaa tacctctagc aaaaatggca gtggaagaga ctggtatggg
      781 tattgttgaa gataaggtta taaaaaacca ttttgcctca gaatatatct ataatgctta
      841 taaagatact aaaacttgtg gtgtcataga aaaagatgaa gcatttggtt ttacaagaat
      901 tgctgagcca gtaggtgttt tatcagcagt tataccaaca actaatccta catcaactgc
      961 aatattcaaa tccctaatag cactaaaaac tagaaatggt ataattttct ctcctcatcc
     1021 aagagctaaa aactgtacta tagaagcagc tagagttgta catgatgcag cagttaaggc
     1081 aggagcacca aaaggactta taggttgggt agatgttcct tcgattgaat taactaatgt
     1141 tgtcatggca gaggcagacc ttatattagc tactggtgga cctggaatgg ttaaatctgc
     1201 atactcttca ggtaaaccag ctgtcggagt tgggcctgga aatgtacctg caataataga
     1261 tgagagtgca gatataaaaa tggctgtaag ttcaatctta gtatcaaaat cattcgataa
     1321 tggtatgata tgtgcttccg agcaagctgt aatagtacca gaaaaaatat atgaagaagt
     1381 taaaaaagaa tttaaatatc gtggagcaca tttcctaaat aaagaagaaa ctgaaaaagt
     1441 aggaaaagta gtaataattg atggttcatt aaatgccaga atagttggac aaccagcaca
     1501 tgtaatagcc aaaatggcgg atgtagaagt tccaaaaaca gctagaataa taattggtga
     1561 agtagaatct gttgaattaa atgaaccttt tgcacatgaa aaactttctc ctgtacttgc
     1621 aatgtacaag tctaaaagtt ttgaagatgc agttgctaaa gcagaaaaat tagttgcaga
     1681 tggaggctat ggtcatacat cttcattata tgcagactct ataaatcatc cagagagagt
     1741 agaaaagttt gtaaatgcta tgaagacttg tagagtatta gttaatactc catcatcaca
     1801 aggtggtata ggtgatttat ataactttaa attggcaccg tctcttactc ttggttgtgg
     1861 ttcttggggt ggaaactcag tatctgaaaa cgttggagta aaacatttgc ttaatatcaa
     1921 gacagtagct gaaaggagag agaatatgct ttggttcaga gcaccagaaa aggtttattt
     1981 caaaaaagga tgtttaggtg tagcagcaag agaatttaag gatgttatgg ataaaaagaa
     2041 agcatttata gtaacagatt ctttcctata taataatgga tatactaaga aattaactga
     2101 tttattagat gagatgggaa ttaagcatac tacattcttt gatgtagctc cagacccaac
     2161 attagcttgt gcaagagaag gagctaaagc tatggctgat ttccaaccag atttaataat
     2221 agctgtaggt ggaggaagtg caatggatgc tggtaagata atgtgggtaa tgtatgagca
     2281 tccagaagta gatttccaag acttagctat gagatttatg gatataagaa aaagagtata
     2341 tgtatttcct aagatgggtg aaaaagcgta ttttgctgct atcccaactt cagcaggaac
     2401 tggttcagaa gtaactccat ttgcagttat aactgaccaa gattcaggtg taaagtaccc
     2461 attagcagat tatgaattaa tgccaaacat ggctataatt gatgctgata tgatgatgga
     2521 gatgccacca agattaacag cagcatcagg tgttgatgct ttaacacatg cattagaagc
     2581 atatgtatct atgttaagaa ctgaaccagc tgacggtcta gcactacaag caggtaaaat
     2641 aatatttgaa tatttacctc gtgcatataa aaatggaaag aatgataaag aagcaagaga
     2701 aaaaatggca atggcatcta ctatggcagg aatgtctttt gcaaatgcct tcttaggaat
     2761 atgtcactct ttagcacata aattaggtgc attccaccat gttcaacacg gtgttgcaaa
     2821 tgcattatta ataaatgaag taatcaagtt caattgtgca gaagctccaa ataaaatggg
     2881 tgcattctca caatacagat atcctgattg catacaaaga tatgctgaat ttgcttcttt
     2941 tgcaggtatt aaaggaagta cagaccaaga aaaagtagat aatcttataa aagcaataga
     3001 tgaactaaag gcaaaagtag gtcttcctaa gactataaaa gaggctggag tagaagaatc
     3061 taaattctta gaaagattgg atgctatggt tgaacaagct tttgatgacc aatgtacagg
     3121 tgctaatcct agatacccat tgatgagtga attaaaagag atatatctaa aagtttatta
     3181 tggtaaataa tatctattgt gcaacatagg ttctattggg tttgtatata aattattaga
     3241 tttgaatata aatagagttt tattagagac acgtagaagt aactataaat aatagattat
     3301 aatttaaatt ctaaatggaa atgataaaaa gtcagtaatt taatggtaat ttcgaatatg
     3361 tgtatttaat caattaactt taaaattagt cggaaaattt ttttattaca tataatttta
     3421 aagcataata tataaaaaac tttatgaaaa aattataaaa atattcagaa aattctattg
     3481 actaatttat ttatcatgat ataataagtt tagaaaagaa aaaaattggg gcctattaag
     3541 gcccccttac tattttttga tagatttatt taaaattact ttctcatttt gattttttat
     3601 atttattata gagtcagtat aatccacttc aaagttggca aaagcagaga ataaatctat
     3661 atctcttaac aaatgaattg tcatacattg attgttctta tttagcctta gaatcaatgc
     3721 tcttacaaat ggaaattgta ttctagaatt aaataagtta gaaccatcag tatctatatc
     3781 taagctacga taattaatat ctataaatat atctacatca tcattttctt ctcttattat
     3841 tctgtagtca aaatccaagt ttaaatttaa attacctaaa atcaataaca tttaaatcct
     3901 cttttctata aatagtttta gagtgttaat ttaagctctc catctattat agatataatt
     3961 ctatctgctt ttctagcaat attttcatta tgtgttatta ttataagggt ttggttatat
     4021 ttttttatag aactttttag taaatccatg acttcttctg tagttttact gtccaaattt
     4081 cctgttggtt catctgctag gattatactt ggttttgcag ataaagcacg tgcaatagca
     4141 actctttgct gttgaccacc agataattgg ttgggaagat gatttttcct ttcattaaga
     4201 cccaataaat ttataaggtc atcaatatat tctttatcta tttttttatt gtctaacttt
     4261 agtggtaatt ctatattttc ctttacattt aatacaggaa ttaagttata ctgttgaaag
     4321 acaaatccta ccttttgtct tctatatctt gctaattctt tatctgaaag acaacatata
     4381 tctttaccat ctacctttat agtaccatta cttggtcttt caagaccacc tactaaatgt
     4441 agaaatgtac ttttcccact tccacttggt ccaattatag caacaaattc gcctttattt
     4501 atttttaaat ttgcattctt tagtgcatgt acagatgttt ccttttttcc ataaatctta
     4561 cctaagttaa tagtttccaa tataggcata attattcctc ctattctgta atgtttatag
     4621 cttcaactat actaatttta tttatctttc ttgatggaat atatgtagct agaataccta
     4681 caattatatt tgctacaaca actagtatat atattttgta atctattgaa aatcccaatc
     4741 catatgatac aaagccctgc atataataca ttttaaattg tattataatt cctgacacga
     4801 tagtaattat acttgagagt acaccatata atattccttc ataaagtatc atatttttaa
     4861 agcctctctc agagatacca atagctctta acattccaaa ttcactagtt cttgaagtta
     4921 agttataact tatgttgttt attatgttaa gtacactgat tatgaacatt attgctacta
     4981 taccataact ataaatcata gttctagatg tcatcttatc agaattttct ttttctacca
     5041 tcatatctac tgttgtagtt ccaggaactt cacttccaat tttacctaaa aggttattta
     5101 tttttttctt gtttgcattt tcattaaggt ctgcataaac taagttatac ttatctacac
     5161 cagttaattt ttttaggtat tcgctactcg ttattacatc tacaccatca tctccagagt
     5221 acataccatt atctgcaaaa ggataactta taatagctcc aactttaaat tcatattcta
     5281 agtattcata attatctttt agactttcat aaagacctgt atccatccca ccctttggat
     5341 atttgacttt tatagtatca cctactttta tatctacagc tggtgttcct tcaccaggag
     5401 taaaagtctt tgtgtatttc tgtgttttag aaatttctgg tacatataca atagctaaat
     5461 tttctttttt catttttttc aagtttatat ttccacttac taagtatttt tctaatgact
     5521 ttatcatctc atcattataa ccttttagct tttgctttag tgtatatttg ccagtagtct
     5581 tattttttat gaacagagga tttagcttat aataaggagt tgaatttata gcttcatagt
     5641 aatctaatct atttatttta tttacctcta ttcttgtgtt taagatacta gcagttttga
     5701 ctttgtcaat tccatctata ttttttattt tatctatctg attatcatta aaccctttta
     5761 tatctttaaa agaagtattg ttaccaattt cattgagata aacatttgat agaataaaat
     5821 ctgcattcat atatgtttgt tcttgagtag atttgttttg cacatctaaa aaagaatatt
     5881 tataattgtc ttttatgacg attatacctc ctatactcat agataataca attataatga
     5941 accccttttt atttctaaag atgtttttcg cagatataga ttttgttact gacatattta
     6001 aagttaattt tgatattaaa taattaactt tggttacatt tttatcagct ccaaaagttt
     6061 ttctgatagc atcaatagga gaaattcttc ttatttttaa gtatgtaaag aaacttataa
     6121 ttagtattat tagtattgta catgcaattg ataagagaat tattttatct ggaataacaa
     6181 aaggtgtaac atttatattt ccttcaaatt gaatatttcc tacagaccta ttaaatattt
     6241 gtgcacctcc catacctata catataccta taggcattgc tattagagta agtatgaaaa
     6301 gttcataaag cataagtttg aaaatcctaa agttggtgga accaatagct cttaagacgc
     6361 catattccta tactctttgg tatattgata taacatagat actatatatt acaataccag
     6421 caaatagact catagcaatt gcaacgtatt tacttgaatt atctaaagta ccgtttttca
     6481 tagtagattc tataagcatt gagtttcttc ctatactttt ctcatctaac atagcatttt
     6541 ttattatatt gtctatgttt gtatttatat cactgccttc attaaattca atataagttg
     6601 taaaatcatt cagttctgat tcattgagat tagaaaaaat ttcacattgt cccttttttt
     6661 tctctataaa tctatcttca aggattccaa caactttaaa agtttctgga ttttcttttt
     6721 gatataattt aaaagtaagg ttttgattta tctctggttt taagcccata ctatttaaaa
     6781 tccatttctc gactaccacc tcattgctag cttttggaaa tcgacctttt ataagtttag
     6841 aatgactagt caaataattt ttatttgcat atagtatgtt tataggtaat ttttcattaa
     6901 catctgtaga cgcatagtaa gatgttatac ccagattttt aatatctttg ctagatttta
     6961 tggactttaa ttgatttttc tttatatttt taaagtatac atggtcagtt ccaagctctc
     7021 ttttcaatct atctacatct gcttgctgag cacttcttga gagggtacca ataccaacaa
     7081 cgagtgatgt agcaagaatt atactcacaa caattgctaa agacctagct ttataacttt
     7141 ttatatacct tagtactatt ttcaaattat ctttcatatt taccacttcc tttcttaact
     7201 aaatcaatta tatatttatc taaaatatat agcaaagtaa gtattttcaa gttttaagta
     7261 gttaattgta caaattcatc tgtattttta caagtttaat taatatatat gtaaatttta
     7321 actttttaca taattttttt aaaatgatat aatccctata taaattgtag aggggagggg
     7381 aatcatggat aacaattttg tatttcctat agtaatacta actttgttta tatttagcaa
     7441 tcttataatg aataagtata aattttacaa agaattagac atattaaaaa gaggcatatt
     7501 aacatttttt atatcgtgtt ttgcattgat tttatttatg gagggcacta aatatataca
     7561 attaaggtta ggatatagtt ttttacttga aggagtatta aatattatac tttttttggt
     7621 tttaattatt ttaagttttt atacaggctt taaagttata agattgataa ataataataa
     7681 tgaacaggaa tataaattac aaaaactaga tagtaaaata aatcataagg ataatataat
     7741 tatcaaatta agaagtcaaa aacatgacta tttgaaacat ttgcaagtag tgtattctct
     7801 attaaatact gggctagatg aagatgccaa agaatatata atgaatatat caaatagctt
     7861 taaatcaaaa aattcaaggt atggaaaagt atcttattta gatgctatag cttcattaaa
     7921 atacgaggaa tgtcttgaaa aggatatatc ttttgatgtc tacattcaag attttatgga
     7981 taatttagag attgaaccta atgatatgag ttcagtacta ttaaacataa tagataatgc
     8041 aatagagtct ttgtctacta taaaaaaaga aaagaagtat ataagattac atgcatatga
     8101 agatgaattt cagtatatta tagctataaa aaataatggt cctaaaatac ataatgttga
     8161 aagtatattt tggaatggat ttagtactaa agaaggacaa gataggggat atgggctaca
     8221 tttagttaaa aatatattag ataagtatga ttatagtata cttgtaaata gtgatgattt
     8281 tttaactgaa tttgtaatct taatcccaaa atattattag ttaggaggag aaaagtctat
     8341 gataaaagtt ttgatttgtg atgatgataa gattgtaaga gatgatatag caaatatagc
     8401 taaagagtct ggatatgtag attatgttaa aaaagttaag gatggtatag aggcaattga
     8461 atttataaaa aatgagaaaa tagatttatt gattattgac attgatatgc catataaaaa
     8521 tgggattgat tctgctgaag aaattctttc tatagataag gatatacata tagtatttgt
     8581 gacgggtttc gctgattata gtcttgaaag ctttaaagtg catcctatag attttgtagt
     8641 taaaccattt aaaaaagaaa aaatacttga ttcaattaac atagcaatag accatattaa
     8701 ctcccataag attgctaata ataattttat agaagacacc ctttttgtat ataaaataag
     8761 gaagcagata catatgatta attttgatga tatagttatg tttgagaaaa attcaagagc
     8821 aattaatcta cacactaaag atgaagatat aataaagttt tatgagaact ttgatgattt
     8881 aaaaaaacgc ctacctccta atttctttat gacccataga caatacatag taaatctaag
     8941 atttatacat aaggttatac ctataaataa atcttcatta gagataaggt ttataaattt
     9001 tcaagaaaat gccatattaa acaaatctct agaaaaagat tttttatata gattttatag
     9061 ggttaaaaga ttctagcgaa gatagaagaa aaatactttt tgtgtgatat aataagtacc
     9121 gtaaagataa tgtataagag aagtttatat aaaaattatt tgtaattatg atttaatcta
     9181 tataaattac agtatagtat atattggact ttctaatttt tttaagaaag tccaatatat
     9241 tttagagata gtttttatac ttataaatta cttaagatac tagggtcttt aatcttacta
     9301 tcttcagcaa gtaatagtat cttagacata atctcagcag ttttagggtc ttcatcagcg
     9361 aatggaagga atatcttacc tctactttga gaatgtatag gaagtatatt caaagcaccc
     9421 atcccttttt tatgtacaat accagaaccc atatgcacag aatactcacc taaattacct
     9481 gctataagag catgagagcc ttcaaaattg acattgttaa tttttaataa taataaaagt
     9541 tctttagcaa ttgctatacg catctctaca gtagaatggc tagtttcagg gtcaacacca
     9601 ccagcatgag cgacacttac aacaaggtct atatcacgca taacttctga gaatagaatt
     9661 ggaggtatag aagctaattc taaactttta taagtttttc tatcaaaaaa ttctactgtt
     9721 tcaatagtag gaggctctat atctgctggt gaaaaccaat ctgcaagtgc aaacatcttt
     9781 gccactatat tttctttata aaatactttc tgtaatccct cttcataatc gacagtccaa
     9841 agctgatttc taagaagtgc tacagttttc tttggctgaa tttggtgacc agcatatctg
     9901 cgagatatag ttttttcaga taattcatca ctatttattc tataatactc acggaatact
     9961 tgcttaaatg gttgaacaat ttgcttacta aataaatcat gttggaactc actccactta
    10021 ccagattcaa acaaatgaac tggatgtgca agttttaatt tattttttgc cgtcaactta
    10081 taagtttcac cttttatatt gcaaataaag aacttagact tatcattata aacatatcca
    10141 atattatcat cacataccca tactagattt ttaatcaaag ggcagataac tggattttta
    10201 tagatattgg caatttcttt aactgtaaat actatttcat ttttcatagc ttgttctaaa
    10261 ctctgttttg cacgagaata ctgactcttt aaatcttttt ggacagattt aagttcaagt
    10321 acatatggat gtttagcaat agcttttggt atactcttta aagacttgtt gcctttttga
    10381 actttagtag atgcttttcc gttttcatca acatctatcc aaattgaagt accatcaata
    10441 tcttttggtt taaatagatg agcaacttca tttagttttt cagtctcaag agcccacatc
    10501 atacgattta catctccaaa tccagcactt ctagctagat tttctagcgc tatagtacaa
    10561 gcttttgatt cactttcacg acgttgtgca ccaaattgct tgctttcttt agcaaacttt
    10621 tgaataaatt cataacggaa agttgcttct tttaggtctc cttttttcat aggaagtaaa
    10681 gagaaacttc tcaatttatc ttgatttcta tttgtttcaa tttctttcat caacatgtct
    10741 ttatcaagct taccaagtgt agcatcagca tagagttgtg cacgtctatg agagttacca
    10801 ccactagtta tatatttagc tgacttgtaa agtatattga atctttcttc cccaatagtt
    10861 ttatatgaat ctaaaaacca atctacatca aatgcaccat caatgaaact acgtggtgaa
    10921 ataggggaga atagagcaac ctctgattct ttttgtgcag agaagccttc atttatatgt
    10981 gcatggaagt accaaattgt acttttcaga cccttccagt ttaaatattt ttcaattatg
    11041 tttacccatt gaggggcata catagcagat tctatcaatc ttaactcagt aatgtcggta
    11101 tttttaagta aactttttaa tttttttgag tcatcttctt tagtaggata gcaattttta
    11161 agtagagaac ttaaaacatc tttttttgaa taattgctac catatgaata tcctctctgg
    11221 aaagtttctt taccaagagc gaccaaaata tttacaaaat aatttatacc ttcaaagtat
    11281 tgtatattat tagctagatt agacacttct gttggcatat cacctctacg tgattcaatt
    11341 tctataattc ggtttgtaac agtttttaaa ttatctttaa gccaagggta cttttctaat
    11401 gtgtcgccag cccatctagg atttgttaat gttcttatta tatttgatgc gttgtatcca
    11461 cttatcattt ctctattaac ttgctcttca gtcattaatc ctaattcatg tgcacgagca
    11521 aacacattta tgctagtata gaaaggaaac ttatattcta gcaactgata atttgaaaac
    11581 gtagtgtaga aaaacttagt aaaagaaata tcatcataaa taaacgaatg tgctttatca
    11641 agccaatagt ttaaaatagg ggcatttaaa gcagggcagt attcattttg tctgtaatag
    11701 tttttagtaa attcaactct ttgtttttta cattgttcta tagtcaataa gtttctaagt
    11761 ttatagtata tttctaaaca atagttaaat atttctttat ggtcaaactc atttagtaaa
    11821 actcttaaaa gttttcttgt aagtccatta aatttaagtt tttcaagttt tgtatatatt
    11881 ttattttgtt ttttagagtc aaggacattt gaatatatag atgaaaacca acttttttct
    11941 gtttcattat atccacatgt acttatacta taaaataaga tatcaagtat taatttgtaa
    12001 tctaagtcaa gttttttact agtttctctc caaagctctg ctaatggata attatcaaga
    12061 gtatcattag aaactttata tttaatagga gtcaagttat aagtagaata acctaaaagt
    12121 actttgtcta cattacctga ccatctttca acttcatatt cataatctgc attttcagta
    12181 ataagctcat caaatttttc ataaactttt attattttat ttatatccgt atcgagaatt
    12241 ttttttgctt ctttttttgt tgctacagaa acagaatcct cacgagtaga taaacttata
    12301 tttgagataa ttttattaag ctttgatgag ctattagaat catctttaaa atcaaattgt
    12361 tctggaattt gaactttgtt ttcagggtta tatattccaa aaccattttc tttgcagtaa
    12421 tcattttttg ctttacctaa caaattatca attagtattt gaacttgaga tgtattcttt
    12481 ttagatttat caatatcttc tataagtaaa acaagcttat tatatagttc ttgtctagca
    12541 gtattgtctt ttaataagtt tacaatttca agacctccaa ggcatttttg agaatttgaa
    12601 gattttatca gtgaagaagc aatattttct aagtttgaat catcttgttc aagtaaaatt
    12661 tttattactg tttgtctaat agagcttgtc tttaaagaca atagattggt tatcttatct
    12721 aattctacat ctgttagctc aagctttcta agtagactca ataccgtttc tctattggaa
    12781 acactcttat cacttaaaca ttccaataaa aattcacgtt gtctatctgt ctttggttca
    12841 tttacaaaat tgactataag tgaagctctt aaatctgaac tcataagatg acaattttcc
    12901 ataagcacat ctataaattg agcattcata tcgtatgctg ttaaagtcat catacattta
    12961 agtatttcat gagaagaaat ttcaatccaa gtccatggaa atacacttcc tgaaaatgat
    13021 tttgtctttg aatttaaaat ttctaaagta tgttttaaac tttcaaattg ttcttttcta
    13081 gcatcaaagt ctttgtcaat aggagtgttt gacaagtcaa aattagtttc tctgttttgc
    13141 caactatagt agtagccaac agaaccttct acatagaaat ttgtaattac ccatgctagt
    13201 atctctaaat catcaccctt aatgttttta gatgctatag aatgtttaaa atatgtactt
    13261 tctgtatgcg ataaaaagta aagagctaca agttttttat atttttcctg acaagaaagt
    13321 aagttttcaa taagagagta agagtcatat atgttgtaag ttgcagtagc ccacaaggca
    13381 aaataaattt caagattatc attgctatta attgattcta tacggaactt ttcatcagtc
    13441 aatgatttat atgaaagttc taaacattta cgtattattc caggtttttg gtctgaaaaa
    13501 ccaagtcctg tccatgtatc caaggctctt ataacagaac taaatcgctc taaattgttg
    13561 tccaaaatag tttttaacat gtatattgat gcagaaattg taccctcatc taaactctct
    13621 acaatttgct gtctaagtcc ttcttgttgt ttggctgcca aaagaagttt agaaagcatt
    13681 tcatagcatt tatcactatg actcttcatg ataccaatta tcatttgtct tgtaagtaga
    13741 gcagtttgat tttctccaaa tattacttct tctaaagcac ttattacttt ttgatttcct
    13801 ttgtctattt ctagtgctat tttatcactc attatagagt catagtacaa gaccttattt
    13861 ggaagagtaa gataatctat aactgaaaaa tcataataaa ttaaatcaac atagtttgaa
    13921 ataaacctta taatacctct aaaataatcc acaagtttat ttgagcgata ccctttacga
    13981 taatatcctc ctgaatatgt ataatccatc tttcttttaa ttaattggca aagttcttct
    14041 acttgttcag aagtaaatat taattctaat gcatttttta aaggtccaga aaggagctcg
    14101 tccatatttt taatttcgta gtagtggcaa ttgtagtatt tctttatcat atttaagaat
    14161 ttgtcttcag gttctctata atagtatgaa ctattttcta tgcagtccca aagagacttt
    14221 gctaagtcgt agtactgttt tgaagaagat ttagtaaaat tatttttaaa attttcaatt
    14281 attttaggat ttttttctac tctagtacgt tctaacattt atgtacctcc aaattattta
    14341 ttttaaaaca ttcttccagc aagcaaggta agtcttataa aagtaaatat atctccactt
    14401 ttcagattta tagaggaatc gagtttttca ataacatctt catttataag cactttgtag
    14461 attccatctt caaatgcctg taacgctact tctattgcct tttctacaga ttgtttattc
    14521 tcattgtaaa tagagccaaa tccaactttt ccaatatcag cttgagattc aatttcttca
    14581 ttagttaaga agtttattat tgatttttct gggattttct cattatattc aagaacattt
    14641 ttagatacaa tttcagagat agcttctttt aggcaattga tgttgtcagg tacatcaaaa
    14701 ggggtaagtt ctagcatagg tttgcgtttg ccaacagcct taactctaaa ataaatttgc
    14761 ataaaatcac ctcgcaaaat attaattatg tatttagaaa tttaaaattt atctatttgt
    14821 tatattatat cattttgtga attattaatg tgaattatgg aattatttta caaaaaatag
    14881 aaaaaaagct atcttaaagt ctaaaaaagc aataaaagat aacttttaac tataattata
    14941 tagaattata actgtacatt tattaaattt taattttaga attatttttg ataactatct
    15001 ttttttattt ataaattttg ctcctaaata ttttttgata tcttcttcag gagaaaagtc
    15061 atcaataagt atttctttcc cagtgtcttc aaatactaca agaacttcac agtcaccttg
    15121 taagaaaaat tttctaactg tatcttcttt aacttctcta acttttatta cattcttttt
    15181 ttctttttca aagtcttttg tgtaataact tcccatcata ttaattattt ttgtatatct
    15241 attcataaaa tacctccata tgcgattaaa tttaaaaaat atgttataat aattgtttgt
    15301 attaacttaa taattataac aaggagtaat aataatgact agtataaaaa ataaagatat
    15361 tttaaattgt atagattatt ccattaaaaa taagctcttt gacaaactaa atgatgtata
    15421 taagtccttg ccagtaggaa attgttctgg atgtggaaat tgttgtatgg agtcagtagg
    15481 tataaattta attgagtttt tgaatatatt ttgttatctt gaagatagag ttgatttaaa
    15541 aaaaagctgt ataagtaaaa tattagatta ttattttgag gagtatacaa gaaagaaccc
    15601 atgtccattt aaagatacta ataatagatg tttaatatat gaagttagac cattaaattg
    15661 taggatgttt ggacattgga aaaaagagga ttataataaa aatttagata atgttattga
    15721 gaaaaataat aactatagag agttaatgaa aaagcaatat ggatttgaaa taaatgatga
    15781 agtagtaaat tacaggatag attattgtga tagatttatt ccaaataaag attatttaag
    15841 taaatctaag agattaagct tttttgatga gcttatgata ttagactcaa atatttattc
    15901 aagtggaaat atagatatag actttaggga tagaggtata gttgaatatt ttatagagtc
    15961 attactttat agaaatttag catataatgt gaaaataagg atatctaaag aatacaaaat
    16021 aagaaaaaga actatagata gaataaaaag gattgttctt gtatagtttt atattagtaa
    16081 gtaataaatt gtatcataaa tattgtcaaa atgaggtgaa gaaggtgaat cttacaaaag
    16141 taaaaggaaa tacctttttt ataaaaggtg gcacgaatac aggaatatat gtatttgaag
    16201 ataacacagc acttatgata gacccagggt tatgtggttc aagacctagg agagtaaata
    16261 aagtattaga tgaaaataat atacaggtta gatatattat aaatactcat ggtcatgatg
    16321 accactatgg agcttgtaat caatttaaag aatattataa agatgtgtgt ataatgtcat
    16381 ctgaatacga taaattatat atagaaaatc cagaattgtt ttcaaagtat attgttgggg
    16441 gaaagagcaa tgtatttatg gataatatct taaaaaataa aatgttggat gatataaaaa
    16501 tagataaatc tatagaagct ggaaaaattg aattaaacaa tgaaatgttt gatataattg
    16561 aatttcaggg tcatacacct ggtagtatag ctgttttgac gaaggataat gtgatttttg
    16621 ttggcgattt attgatagga gatgaaattt tatcaaaata cgatttcttg tttctatatg
    16681 atatacaaga acaaataaat tcattagaaa aattaaaaaa tatagatttt gagtacttag
    16741 ttttagggca tggcaagcaa gttatatcga aagaagattc ttataaatta atagaaaaac
    16801 atttgaatgc tttaaaaaaa tatttatatc aaataagaga gttgcttgta gaaccgaaat
    16861 ctttagaaat tttattaaaa aacattataa ataacaataa tttaagcaat aattataaag
    16921 agtatcattt ttttaaatct tcgttagtat ctgtcataag ttacttagtt gatttagaag
    16981 aaatagggta tatattggaa aatggagaat tgctttacta tacaaaaaca aaataaatta
    17041 tgataaaata aagaaggtca taaaaaacaa ataaagggtg agcaatatgg aatacgaaaa
    17101 atggaatgag atactagcac cttatgaaca tgcagtagag gaattgaaaa taaaatttaa
    17161 aaatataaga aaagaatatt tagcaaaagg tgaatattct cccatagagt ttgtcacagg
    17221 tagaacaaaa aaaatatctt ccataatatc taagttaaaa aggataaatg caaaagatat
    17281 agaaactgaa attgatgata ttgctggtat aagaataatg tgccagtttg ttgaggatat
    17341 atatgctata gttgatttga taaaagtaag aaatgatatg actataatag gagaaaaaga
    17401 ttatattaca aattacaaag atagtggtta tagaagctat catgttataa taaaataccc
    17461 tataaactcc attgcgggtt caaaggaaat tatatgtgaa atacaaataa gaactcttgc
    17521 aatgaatttc tgggctacaa tagaacattc attaaaatat aaatatgacc attacatacc
    17581 tgagactctt gctgaaaggc ttagaagagc atctgatgct gcctttcttt tagaccaaga
    17641 aatgagtgaa ataagagagg acataatgaa agctcaggct atgtaccaaa tgaagtctat
    17701 agctattaga gatactctaa atagaataca agagttatat gacttgggtg atacacataa
    17761 agctatgcag taccaaagaa aacttgatag aactgaaaca gataaaaata taactgaaat
    17821 tttggaatta aaacaagaaa tagatatatt attagagcaa tataaggata ctaaggtaga
    17881 tgataaagta gttcagtaaa aactacttgt agaaaatttg ctaaatatta atggggctgc
    17941 ctcttaatgg aaaataattt ctaaaaagag acagcctatt tagtgtaatt aagaaaggaa
    18001 ggatattatg agtttgtatg ctataggtga tttacatttt tcaacatcag tcaataagcc
    18061 aatgaatatc tttggaagta attgggatgg tcatgaaaag aaaattatag ataattggaa
    18121 agaagttgtt aaggaagaag atatggtctt agtactagga gatacatctt ggggaattaa
    18181 tttatccgaa gctaaaaaag atttagatat aataagcaaa cttccaggtc agaaaatact
    18241 tataaaagga aatcatgatt actggtggac aaccgttaca agtttaaaca agctatatga
    18301 agacatgcgt tttattcaga caaattttta tgaatataaa gattatgcaa tttgtggagg
    18361 taggggctgg atatgtccta atgatgtcaa atttgatgag actgatgaaa aagtttataa
    18421 aagagaagaa catagactta gactatcatt agaatctgct cgtaaaagtg gtcactcaaa
    18481 aataattgtg attactcatt atcctcctac gaatgataaa ttggaagaat ctttatttac
    18541 aaagttattt gaagaatata atgttgaaaa ggtgatttat ggtcacttgc atggtaaaga
    18601 atcttttaaa atgggattga aggggattcg aaatggagta gaatatacat tagcatcttg
    18661 tgactataca gaatttaatt taataaaagt tcacgattaa aaaacataaa ttggtaaata
    18721 ctataagata catgattaaa aataaatatt tgagggattt cggttttgag aaaggagaca
    18781 cgacttatga ggtggatatt agtttttata tttttatatc ttattccttt agtagtgata
    18841 ttcagaaatt acaagaattt taagaggtct tgtatctaca gtagtattta tgtggtatta
    18901 gctacaacaa taatgataag taatatttat atgagtggac ttaataaaat aaaagagtct
    18961 atgtattatc acaattatat ttctgatgag acatatgatg aaaagtatgt atcaaatttt
    19021 aaggaaaaag aaagtactga tttaaaacag gataataaaa agaatgaaga aatatcaaat
    19081 aataaaaatt taaacaacaa acaagataga aatatacaaa ataaagaaag taatgaagaa
    19141 ggaagtaatg atataaaaga aaagaataat tctgatagta atgtttcagt gaataataaa
    19201 tcagaaaaac aagaagataa tacaaaagaa aataataaat atacaagtaa agaaaaaaat
    19261 agtgaagata taaaaaaaat agatatggaa agtataggaa tttttaagaa agaaatttat
    19321 aatatagaaa gagcagcttt agttccaatg agagaatgca taccttacac taaggatata
    19381 gcaaaaaata taactaaatt atctagtata aaaaaagatg ttacaaatgc cagaaacaag
    19441 tgtaaagaag ttgtaaaaga atatgaaaat atgcagattc cagaactttc taaggagaag
    19501 tatacagaaa cattgagtaa tgctagagat gacgtaaaga aagcatatga gcttagagaa
    19561 aaatctatgg aaagtgcttt gaaattaata aatactaaaa gccctaaata tatagggaag
    19621 ataacagaat atcttgattt atcagataag cagatagaaa gttttaaaaa tagattgaac
    19681 gatttaaaag aagaaataaa taattcagaa acaaataata ttaattaata agatttaagg
    19741 atataagtta aaagggagat tatgatgaat aaacagagta taggaaaact aaatatgttt
    19801 tttatgggga ttactcaaag aaaccttgat aatagggatt tttttattga gttagaggtg
    19861 atttttaaat ctggaagaaa agagtttaaa ggaattgcaa tagaggaaga taataaatat
    19921 aaatttaatt ttaaaggaaa atcagattta tatacgttta atgagttgct taagaatatt
    19981 tctaaagagg cagaaaatta tgatggatta gtatttaaat atgttgagcg aggaactatt
    20041 gttgtaattg aaggagacaa taagaaggta aatgtaaaat atttagataa taaggaagaa
    20101 gtacctaaaa tagatgaatt tacggcatct caaataaaaa acagagatta ttatgtaaaa
    20161 gtaggacaag caaatgcact tttaaaagaa attggagtac ttacaaaaga tggtaagata
    20221 aaaaatgata aaataagaaa atacaatcag atagaccatt ttgttgaatt gatagatagt
    20281 attttaaaag aaataaagga taaagactgt ataactatct tggattgtgc ttgtggaaaa
    20341 tcttacttaa gctttgtctt aaatttttat ataaaagaag tattgaagaa aaactgttat
    20401 tttataggaa tagattattc agatgtagtt atagaagcat caaaaaatat ggctaaaaac
    20461 ttgggttata aaaatatgtc atttataaaa gaggatttga ctaattatac acctaatagg
    20521 gatgttgacc ttgtaataag tctacatgct tgtgatacag ctacagatat ggcaataggt
    20581 ctaggaatta gagcaaaatc tgaagctata gtagtagtac catgttgtca taaggagtta
    20641 ttagggcaat atagatatga agctatggaa ccaatattaa aacatggtgt atttaaagct
    20701 agatttgcag atttaataac tgatggttta agaacattgt tattggaggg caatggatat
    20761 gatacttctg tagtagaata catatctcca cttgatacac caaaaaattt aatgataaga
    20821 gcgataaaaa ctaaaacgaa taatgacaaa gcattaaaag aatataaaga actgaaatca
    20881 caatttggag tggaaccaac cttagaaaaa cttatatatt agttaaaaat gtaaaattta
    20941 cgaaaagctt gcttaaatat gcaagctttt tggtattata taggtatagt catatccaga
    21001 gttttgattt ataattaaag ctgtgctata atatttgatt aatcaaatca ataaatgtta
    21061 catggaggta aaaatgaaca aattgaatat atgtattgat atagatggaa caataacaag
    21121 cccttatcat tttcttccat acctaaatga tatctttaat aaaaatataa ctgaagcaga
    21181 gtgtataaca cataattggg aagaattgta tggttcggga ataagagatg tgtatgcgga
    21241 gtttaataat aagtatatac actcatatga tgaagcaaag attgtagagg gtgctacaga
    21301 ggtaattagc acattatcta aaggacataa tttatctttt gtaacggcta gacatgagtg
    21361 tttaactgat gtaactaaaa attggctatc gagacaagga ttttcagata tagatgttta
    21421 cttattagga agtgattata aagttgaaaa ggcaagagaa ctgagttgtg acatatttat
    21481 agaagataat cctttaaact cagtgcaact agctgatgat ggtgttaaag taatacttct
    21541 ggatactaac tataataaag atgttaaaca tcaaaatata gttagagtta gtaattggat
    21601 agatattgaa aagattataa aacaaggtat ttaaagattt gtagattgaa agggggagca
    21661 tatgatagat ttaaaaattg aaaatagaaa aggaaaaatc catgtaaaga gcagtgaaat
    21721 aaaagacatt ttagaattac gtccagactt cgaatatgta caagatatat caaatactat
    21781 aaatcaagaa aatatattgg tatttgattg ccagttgaca ggaaattctt ttgatatgga
    21841 tgatttagat atagaagaaa tattggaaga gttaggtgaa gaaatagatg agtcttattt
    21901 cagtgtttta tttgaagatg taagagcgta tttaaaagat gctactgatg agatagaagc
    21961 agacttacag gataattact tatttgataa tttgagatgt tattttgata tatacaatat
    22021 aaatcaagaa tttacagatt ttaagtttgt atttttagtt tcttttaaag atataaaaat
    22081 ttcttcatta gcaaacctag ctaagattgt ttctaagaga cagcttatag gtgcatctaa
    22141 gttttatagt taaaatgaaa gattattata ataaaagcta gtaaccttgt aaaaatatag
    22201 gttactagtt ttttatttat tcattttcac aaaatttcag aaaactattt acattctaaa
    22261 aaaagtaatg tatattaaac ataatgtaca ttataatgga taaaaagttt ctattaaatt
    22321 actaagttta tttaataatc aaaataattt atcttatatt agtatgtatt agggggtatt
    22381 tatgcaaagt ttaaatgaaa taattgctga aaatttaaaa aagataagga aagaaaaaca
    22441 tcttagttta gacaagattg cacaacttag tggtgtaagt aagagtatgc tatctcaaat
    22501 tgaaaagtgt gaggtaaatc ctactatatc tactttgaag aaaataacta atgggctaaa
    22561 aatctcattt acatcattga tggagaggca ggaaagtgac atagaattga ttcaaaaaag
    22621 tgatatagac cattttattg aagataatgg aaagtatata agttatccaa tatttccatt
    22681 tgactccaaa agaagatttg agatatttat gattgaaata gaagaaggtg gaaatttaga
    22741 ttcaaatgct gaacttccag gaacacaaga gtttataact gtttttagtg gcgaagtaac
    22801 cattaaaata aatggagaag attacattgt atctagtgga aattctataa gatttaaagc
    22861 tgatgtatca catatatata gaaactcagg caaaggtata gctaaaatga gtatggtagt
    22921 atattatata taaataattt aatggaatta gagtgatatt tagatgattt taggtatgaa
    22981 gatatattat atttaagtta taggagtgtg atttatggat gaataataaa aaagcagttt
    23041 ttttcttttg cagtgattta aagaaagacc cagtagccag taatgtgata aaatgttgtg
    23101 aagaaataat ggatttaaat caaactgata ttgttattga tgataatttt gtattagagt
    23161 ttaaggataa aaacaataac ttatttcatt ttgtaaaaac aaaagatgta ataagtcata
    23221 attataaata ttatcttcca attttaaata gacattttag agattatgat tttgcaggag
    23281 ttgtaaattg gcatgaggga gaaaatgctc cagaacatat cctaacagct catacaattg
    23341 gagatgttcc aacaggagaa tttggaaatt caaatccaag atactttaaa aatctaatca
    23401 atgctattga ggatataaaa aatgaaaatc tacttgatga atttactaca ttaacagaag
    23461 caactcactg gtctggaaca acatatggtg aagaatctaa attaattaca gaatattctg
    23521 tacctatgtt ggatattgag ataggaagta gtagtgatag ttttaataat agtatagcaa
    23581 tacaggtatt ggcaaaatca ttaattagag tatttgattg tgatgaacct ttgaagactt
    23641 tgttatgtgt tggaggggta cattttgaga aatctttctc agatattata aaaaataaag
    23701 aatataacat atcaattgga catgtattgc caaatcaatg gattgtaagt ggaatgtatg
    23761 atgatgaatc tggatttgag aaactagaaa agtgtataaa tagtattgaa ggaggaattg
    23821 attgtatagt ttttcatgat aaattaaaag gtacctataa agagcaatgc cgtaaactag
    23881 gtgaaaaact taatgtacct gtatttaaac ataaaatatt aaaaaatcca aaagacttac
    23941 ctatatggta gtcttataca gttaatataa caataagtcg gattaatttt taagcgaggt
    24001 caaaaatgat attatcttat tttgcattat taattagagt agtgctatta gcttttgaaa
    24061 gagtagttgt aagattatta ggtgatgaag aaggagatat ttataaaaat atagcttcat
    24121 catttctatt tttctttata gggggagtat gtttacttcc atttagcata atggatagag
    24181 ttagtgattg gtcgttttta ataccatgtt atataagtag tttagtctat tcaattggtt
    24241 cagtagcgta tgtaacgtct ttagcaactg gggaagtttc acttgttact ccaataaata
    24301 gtttaaattc attgttttta ctgcttttat cagttatatt tttaggtgaa ggtttaagtt
    24361 tagcaaaagt tgcaggaatc ataattatga ttggtggagt gtttatatta aaaaaagtaa
    24421 gctctttttt aaagaatgtt tctgtaattt ttaacaattt accatgtaga ttaatgtttt
    24481 tatacatatt tttgcagtca gtaggaagag tattggataa ggcttttttt gtacaagtat
    24541 ctcctatatt atattcaact atactgtatt ttttcgtagg tttaaattta tttatctttt
    24601 tagcatttaa aaagaaacaa aaaataattt gtgagatttt tactaataaa aaggggcttt
    24661 caattttaag tggtgctatt aatggatatt cttatttagc attattaatt gctttaaata
    24721 atctagaact tagtatagca gagccatttt ctcaagtatc tatgataata actttaatac
    24781 ttgctcattt tatattcaaa gaaaatataa aagaaaaaat accaggttca attttgatac
    24841 taattggtgg gtggctactg ttactttaat tgacataaaa aatgaattat aaaggtgcta
    24901 ttagttagta tcagaaaatt taaaaaagta ttgacaattc ttttaggcaa atgttaatat
    24961 aataatacaa aatagtataa atagaagaca tgttactggt aatgcaggca ggatcgaata
    25021 tattgctttg atttttcaag gtgtttatat aggttctgcc ttttttgcat cttgaatggc
    25081 cattcataaa aaattatgga gaaggagatt acaagatgaa ttataaaaaa actgccattg
    25141 acattattga acaagttggt ggttcaaaaa atattttagc tttagaacac tgttcaacta
    25201 gactacgctt taagttgatt gatagaagta aagtcaatca ggatgcttta aaaaaagtac
    25261 aaggtgttat gagtatagtt aatggggcac aatttcaagt tgtcattggg aatagtgttg
    25321 ttgatgtgta tgatgaaatt ttaaaagttt gccctttaga tacaaatgat agtactgtaa
    25381 aaactgaaga aaaaataaac ttaggaaaaa gatttataga atttattatt aatatttttc
    25441 aaccactgat ttttgctatt gcaggagctg gtattttaaa atcactgttg atgttattag
    25501 caatgtttaa tattttaaaa tcagattcta ctatatatac attattagtt gctatttcag
    25561 atgcaacatt ctatttccta cctattatgg tagctgtaac tactgccaat gtattgaaat
    25621 gtaatcgttt agtagctatt gccgctgtag gatacttatt gcttccagca acaacaactg
    25681 ctttaagtga aggtgtcgaa ctatttggat ttacaattcc aaacattgcc tataatgcac
    25741 aggttttccc agctatctta tgtgtgtctt tcttggcaac aatggagaaa atttttaaca
    25801 agtattcacc aaaaccaatt cgaactttct ttgttcctat gatgtctttg gcaattactg
    25861 ttccaattac tctaactatt ttaggtcctt taggatataa cgttggtaca atttttacaa
    25921 ccattatttt attcttatat aacaaaatgg gattcttagt tgtaggatta cttgctgctt
    25981 ttcttccatt tatggttgca acaggaatgc acaaagcact aataccttat tcaattaata
    26041 caattggaaa gttgggttat gaagctttat atatgacatc ttcattagcc cacaatattt
    26101 ctgaaagtgg tgcttgtttt gcagtagcct tgcgaacaaa agatgaaaca ttaaaacaaa
    26161 cagcattatc tgcaggaatt tctgccttga tgggaattac agagccagct ttatatggta
    26221 ttactttaca acataaaaga gcaatgttag gtgttgtatt ctcaagtgca atttctggta
    26281 catttttagg attatttgca gtaaaaggat ttgtaattgt tggaccaggg cttgccagta
    26341 tgagtatgtt tattgaccca aacaatggaa acaatttgat ttttgcaagt attggttttg
    26401 ttattgcaat attaggctcg tttattatta cactgattat ttggaaggaa gatacaaata
    26461 cagtagagtc atcaattgga gatgttgttg aagaagagct agaagaagaa aaagaagtaa
    26521 atatcttagt aactccagta gaaggaaaag taatagattt aagtaaggtt aatgatgaac
    26581 tatttgcaag taagactttg ggagatggag ttgcaattat accaaccaat ggaaatttat
    26641 acgcaccttg tgattcagaa gttgtgatgt tatttgaaac aaaacatgca attggtttaa
    26701 gaacaaaaaa tggggctgag attttgattc atattggaat aaatactgta agtatgaatg
    26761 gagatggatt taaaacattt gttaagacag gcgataatgt aaaagaagga gatttattaa
    26821 ttcaatttga tttagacaaa atctcacatg ctaatttaga ttcaactgtt atgattgtaa
    26881 acaataatgg agcagattat gcttataaag tgttaaacca gtcttatggc aatgtaaaaa
    26941 aaggaagtat cttgtttgat gtaaagggag gaatataaat atgagaaagt ttccagaagg
    27001 atttctttgg ggaggagcta cagctgctaa tcaatttgaa ggtggttgga atttaggtgg
    27061 gaaagggtgg tctgtatcag atgtagcaaa ggcacacttt gatgcagatg tgaaagatta
    27121 taaatctaat aatgaaatca caacaaaaga tatagaagaa ggattagcac atcctgaaga
    27181 tgaagtaaat tatccaaaac gtcatggtag tgatttttat catcactata aagaagatat
    27241 tgctctaatg gcagaaatgg gatttaagac atatcgaatg agtattgctt ggtctcgtat
    27301 ttttccaaat ggagatgata aagagccaaa tgaagaaggt ttacagtttt atgatgatgt
    27361 atttgatgag ttgataagtt atggaattga accacttgtt actatgtcac attatgaacc
    27421 accattaaat atcgttttaa actatgatgg atggtattca cgacaagtta tcaatatgtt
    27481 tgttaggtat gtagaaacta tctgtgaaag atataaaaac aaagtgaaat attggcttac
    27541 atttaatgaa gttgattcta tgattcgtca tccgtacact actggtggtt tagttcgaga
    27601 ccgttttaag gataaaaact ttgaggaagt tatttttcaa gctatgcatc atcaatttgt
    27661 agctagtgcc cttgcaacaa aaatttgtca tgaaattatt ccaaattcaa aagtagggtg
    27721 tatgttaaca aaattaacgt attatccata cacatgtaga ccagaagatg ttttggcaac
    27781 tcaacaaaaa atgagaagta tttatgcata tagtgatacg caggtttttg gtgagtatcc
    27841 agtatattta ctgtcgtatt ttaaaaataa taacattcaa atagtaaaag aagaacatga
    27901 tgatgaaatt atgaaaaagt atccagtaga ttttatttca ttctcttact atatgtcttc
    27961 ttgtgaggca gctgatacaa ctggattaga tattacacct gggaatacgt tattagctgt
    28021 aaaaaatcct tatttagaaa tgagtgaatg gggttggcaa attgattcaa taggtctacg
    28081 tatatctttg attgaattat atgatagata cagaaagcct ttatttatag ttgaaaatgg
    28141 attgggagct aaagatattt taactgaaga taaaaaagtt catgaccaat atagaattaa
    28201 ttatttaaaa gaacacttta aatgtatgtt agatgcgatt attgaagatg gtgttgaact
    28261 ttggggatac acttcatggg gttgtattga tttggtcagt gaatcaacga aacaaatgtc
    28321 aaaacgttat ggatatattt atgtggatgc tgatgattat ggaaagggga catataatcg
    28381 atataagaaa gatagttttt attggtataa aaaagtaatt gaaaataata gtatagattt
    28441 taaatgacca atagaaggtg agcaaataaa atgaactaca taataaagaa agttttgaac
    28501 tcgagcgttg ttttagttca tgacacaaag gataatgaat ttattttgct agggaaagga
    28561 attggatatg gtaaaaaaac aggagaatat atttgtggtt ccaatgataa tcaaatgttt
    28621 gtccctgttg aaaatacaaa atcaaaacaa tttttagaat taatggatga tattcctgtc
    28681 gacattttaa agataacaca ggaaataata gtagaggcag aaaagttatt gaactcatct
    28741 tttaataaaa acttatactt aatattagca gaccatttta actttgcaat tgaaaggatg
    28801 cgtaaaggaa taaaaattac aaatcgagta ttttgggaaa taaaaaatta ttatccaaat
    28861 gagtttaagg ttggaatggt tgcaatttat atggtagagg agagattggg cattcaatta
    28921 ccagaggaag aagcagctaa cattgctttc cattttgcaa atgctatggc ttcagatgga
    28981 aattcatatg atacgattaa gtatgccaaa gtaattggag aaataattaa tatttttgtt
    29041 ttttctttga atagaacctt agataaaaag agtatgcatt atatgagatt tataacacat
    29101 ataaaatttt ttgtggaaag atttttttca gatagtatgt taagcagtgg tgatgattta
    29161 ttatttaatc aaatgaaaag gtcaaatcca aaagaaatgt taattgcatt aaaagttaga
    29221 gattttttag aaaagaaata tggaaaaaag ttaacaaatg aggaaatagc tttcttggtt
    29281 gtccatatcg cacgagttaa gcaatagagt gaattacagc aatagagtaa attacagcaa
    29341 tagagtgaat tactaatatt atcttgtacg tctacatggt agaaaattga aagaaggtaa
    29401 agtgtataaa gaaactgaga catattattt tgttgttcat tatgtttatt ttatgagtgt
    29461 atcctgttaa aaatttaagt taatatttat aattaaaata tac
//
LOCUS       DAMJYC010000047        29085 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJYC010000047 DAMJYC010000000
VERSION     DAMJYC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.048"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            33..884
                     /locus_tag="PC472_002186"
     CDS             33..884
                     /locus_tag="PC472_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2727509.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1017..2630)
                     /gene="ggt"
                     /locus_tag="PC472_002187"
     CDS             complement(1017..2630)
                     /gene="ggt"
                     /locus_tag="PC472_002187"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2727510.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2855..4792)
                     /locus_tag="PC472_002188"
     CDS             complement(2855..4792)
                     /locus_tag="PC472_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727511.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSILAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKRELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4779..5546)
                     /locus_tag="PC472_002189"
     CDS             complement(4779..5546)
                     /locus_tag="PC472_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727512.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5834..6508
                     /locus_tag="PC472_002190"
     CDS             5834..6508
                     /locus_tag="PC472_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727513.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6532..7536
                     /locus_tag="PC472_002191"
     CDS             6532..7536
                     /locus_tag="PC472_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727514.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7648..8877
                     /locus_tag="PC472_002192"
     CDS             7648..8877
                     /locus_tag="PC472_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2727515.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIISEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8885..9559
                     /locus_tag="PC472_002193"
     CDS             8885..9559
                     /locus_tag="PC472_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727516.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9562..10794
                     /locus_tag="PC472_002194"
     CDS             9562..10794
                     /locus_tag="PC472_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727517.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11080..12345)
                     /locus_tag="PC472_002195"
     CDS             complement(11080..12345)
                     /locus_tag="PC472_002195"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2727518.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12398..13546)
                     /locus_tag="PC472_002196"
     CDS             complement(12398..13546)
                     /locus_tag="PC472_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2727519.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13637..14182)
                     /locus_tag="PC472_002197"
     CDS             complement(13637..14182)
                     /locus_tag="PC472_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2727520.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14405..15349)
                     /locus_tag="PC472_002198"
     CDS             complement(14405..15349)
                     /locus_tag="PC472_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2727521.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15735..16616
                     /locus_tag="PC472_002199"
     CDS             15735..16616
                     /locus_tag="PC472_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2727522.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16677..17885)
                     /locus_tag="PC472_002200"
     CDS             complement(16677..17885)
                     /locus_tag="PC472_002200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2727523.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17958..18210)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18362..19351)
                     /locus_tag="PC472_002201"
     CDS             complement(18362..19351)
                     /locus_tag="PC472_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2727524.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19503..19943
                     /locus_tag="PC472_002202"
     CDS             19503..19943
                     /locus_tag="PC472_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2727525.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVYIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20269..20496
                     /locus_tag="PC472_002203"
     CDS             20269..20496
                     /locus_tag="PC472_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2727526.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20522..22642
                     /gene="feoB"
                     /locus_tag="PC472_002204"
     CDS             20522..22642
                     /gene="feoB"
                     /locus_tag="PC472_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2727527.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKENQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22775..23593)
                     /locus_tag="PC472_002205"
     CDS             complement(22775..23593)
                     /locus_tag="PC472_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2727528.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23801..24085
                     /locus_tag="PC472_002206"
                     /pseudo
     CDS             23801..24085
                     /locus_tag="PC472_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     repeat_region   24243..26118
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24310..24338
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(26777..28870)
                     /locus_tag="PC472_002207"
     CDS             complement(26777..28870)
                     /locus_tag="PC472_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2727529.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT        10296 a         4392 c         3548 g        10849 t
ORIGIN      
        1 taaagataat ataaacacag gaggtacatt ctttgaaaaa cattatttca gcaaaagaac
       61 ttatatcaaa actagaaaaa aatgataatc tagttgtaat agattgtaga tttgatctta
      121 tcaatagaac ttatggtatt gattcttata aaaaaggaca catcaaaggc agttttatct
      181 tagatataga caaggattta tcatctccta caaaagaaca tggtggtaaa aatcctttac
      241 aagacccttt aatactaaaa gaaaaattag aaaaaatggg tgttgataat gacactacga
      301 ttgtaactta tgatgatgga gatttaaatg gagcttgcag attatttttc caattaaaac
      361 atttggggct taaaaatgtc tatgtactag atgggggaat aacatctttc gtaaaggaag
      421 gtggagaatt ggaagagaaa gtgaatatac caagttgtac aggtaaggaa attagaccaa
      481 atataaacaa tgatttagtt gttccaatgg aatatgttaa gtctaaatta tacaaaaaag
      541 atactgtaat aatagattgt cgtgctaatg agagatacca gggtttagtt gaacctgctt
      601 actcaaaagc tggtcatata ccaagtgcaa aaaattattt ttgtaaggat ttaataaaaa
      661 gtgactttga aaatggctca cttaaagata tagaattttt aaaaaaattc tttaaagatt
      721 taaataatta tgatgaagta attttatctt gtggttcagg gatatctgct tgtgttaaca
      781 gtcttgctct tagagaactg gatatacctc acaagattta tattggtagc tttagtgact
      841 ggattagtta tgatgacaat gaaatcaaaa ctggtcaaga ataaggctca taatctatat
      901 aaaatcagca tatgatagct taagttttgt aaaggcgtca gtcattgtag actttcgcct
      961 ttacttatta tttcatatac tctttaaatt tattatatat attttagttg ataatattaa
     1021 taaactgcaa tatgaccatc acatcttgat tccgtcccac aaatataact ttcctgttct
     1081 gttttaaata ttatttgacc tcgtcccatg tcaacagtat catgaattac ttttatttca
     1141 tgaccctttt caattaatga atctattata tgctttggca tctctggttc aacttctatt
     1201 tcttttcctt ttatccactg ccatcttgga gcatctagcg cctcttgtgg atttaatcca
     1261 aaatcaatca tatttgttaa cacttgaata tgtccttgtg gttgcataaa agctcccatt
     1321 acaccaaatg gacctattgc tttatcttct ttacctaaaa atccaggtat tatagtatga
     1381 taaggttttt taaatggttt tactacattg tggtgtttag ggtctaaatt aaaattattt
     1441 cctctactat gtaaagcaat cccagttctt ggtatcacaa ttcctgaacc aaaatttatg
     1501 taattgctct gtatatatga aaccatattt ccatccttat ctgcagtaca aaggtatact
     1561 gtaccaccac aatatggctc tcctgcagtt ggatacaaag cctctttatt gtcaatcaat
     1621 aaacttcttt ttttggcata ctcttggctt agtaattcct gaatctttac cttcatatgc
     1681 tctatatcag ttacatatgt tttagaatct gcaaaagcta attttaaact ctctataatt
     1741 ttatgtatgt cttctacatt ttctatattt ccttctaatt ccaattcttt taatatgtta
     1801 agtgccatta aaactgttat tccatgacca tttggtggga tttcaaatat tttatatccc
     1861 ttatattctg tacttatagg ttctacccat gttggataaa aacactctaa gtcactcttt
     1921 cttatagccc cattaaattt tttagaatat tcatctattt tatcagcaag tcttcctcta
     1981 taaaagcttt cagcctgagt atttgctatc tcttctaatg tacttgcctg ttcttcacat
     2041 ataaatatat ctccagcctc tggagcttta ccatctttag aaaatgtatc aaaccatggt
     2101 ttgaactctt ctccaataag ttctttttca tagagttcat atgatttttt ccatacctta
     2161 gctacattag gagatactac gtatccctct cttgcatagt ttatcgctgg agataagcat
     2221 tccattaagc taagtttacc atatttttta tttaattctg accaagctgc tggtattcca
     2281 ggtacagtta ctgctccaaa cccatatttt ggcatttctt tcatattatt ataatttttt
     2341 aattccatat tttctggagc aaagccacta gaatttaaac catacaatcg tttttcttcc
     2401 tctatccaaa caagggcata tgcatctcct ccaatcccat tgcttgtagg ttctactact
     2461 gctaatgtgg cggctgttgc aactgctgca tcaacagcat ttccaccctt ttttagtatt
     2521 tcaagtccag cttgagatgc taatggtgtt gatgttgcaa ctgctccatt tttagcatac
     2581 acgacatttc ttattgatga gtatttatgt tggtaggcat taaacttcat aaatttcagc
     2641 tcctcgtcat tttttatttg atattctgct tagatttatt atataacatc aagtaatgta
     2701 actgatacaa aatgatataa aatatagact ttttcctaac tttaatttta tttttaaata
     2761 aatatcttaa actcaattct agtattttct atctgtactt aaataagtta ttttattcaa
     2821 aacatatatc taattctttt aacttaaaaa taatttattc ttttatcatg tttagagcat
     2881 attttaaagt taaaaaataa tatataaaat atcctataaa ataaatgact agagttatga
     2941 taattggtac aagtaatgac cttggtataa acttttgtgc tatagacata gcaaataaat
     3001 tatgaactgt acctacaact aatggcatta aaaatataac ttttaactgt tttgatatta
     3061 ttttctcaat atctttatta tttgctccaa gttttattaa cataatatat ctttccttat
     3121 catcatatat atttgataat tgtttaaata agattatact acctgtacat aaaagaaata
     3181 ctacagacaa aaatatgcct ataaacagca ttagaccact taattttatc aagttattat
     3241 aactttcaat acttgatgta aaattaaaag gataagtaaa tttcatattt tcacttacta
     3301 tattttttaa ttttaaagtc aaatccagtt ctcttttttc attttttata tcaattaatc
     3361 ttattaatct agaatttcca tgtaatttta actgattaaa tacgtcatct tttactacaa
     3421 ttaaatcaaa cgtagcttgt gaatttattt ctggctccaa gatgcttttt tgtactttaa
     3481 acagatttat atcatactca tttgtatctt taatatgcaa gtttattttt tcaatctcaa
     3541 agtctttaaa aaatattttt ttgtatgaat cagaaacata gtacacagat tcctttgaag
     3601 ttaattcttc ataattgtta ttctgatatt gtcttaattt tttaaaatca ctttctctaa
     3661 taatatctac agacgttcta tcttctatat aactatttcc atttttataa tacatttcaa
     3721 tatttatatc tttgtctata agttctattg ttttatcaaa gattatctta ttatcttttt
     3781 tatatttata taataatttg tcaatttcgt tatttacata agaatttcct aaatttatgg
     3841 tataactgta tttataattt tcacttatgt tcctatctat atcgtaataa agtgatatgg
     3901 taaaccctag catagttaca cttgtagcta ttaatattgc tattacagcc aatatactac
     3961 tattgctctt taatttaaat ccaagttcag aaaatgcaat taaattctgt cccttataat
     4021 aaaaactttt tctcttctta acagcatcaa taatcatata catcgttgat gaaaaaaata
     4081 aaaaagttcc aggtattatt gtaactaata taaaaactga aagcataata ttttcagcaa
     4141 taaaataact aattgacatt aaataagaaa tagctattaa taaaacacca agtacacatt
     4201 ttaactttat aaatttttta aacttatact tttctggata tttattaaat aactctataa
     4261 ttctcttatt tctgataact atagtatttc ttattccaat aactatgaat attattaaaa
     4321 ataccgctag tgtctgaact aaggccttta tacttaagtt catatttact actatcattt
     4381 cattcattaa ttttacaagc atcatagtca taagctttga aaataacagt cctaaaaata
     4441 tacctattag tattgcaact atacccatca aaaaagtttc atataaaaat atttttccta
     4501 tctgattttt cctcattcca agtaaactat aagttcctac ttctcgccta cgtctattga
     4561 aaaaaaatga gtttgaataa tatataaata aaaatacaaa taccactatt acaattgaac
     4621 ttgtattaat actcatccta gttttctctc caagtgaact caacgcttca ttatactcta
     4681 tagatttaaa actaaaaaat acaaatacac taaatacaat tgaaataaag tacattaaat
     4741 aattaaaaaa actattcttt acattttgca ttgcaattct aaataaagtc atttttatta
     4801 actcctccaa ttaaggaaac tgtatttact attttattat agaactctct gttactttct
     4861 ccctttacaa tttctgtgaa tattattcca tcctttataa agagtattct attgcaaaaa
     4921 ctcgcactta tagcatcatg agttaccatc aatattgttt tattgctgtc tttgtttaat
     4981 tttaatagac aattcaacaa atccctagca gatttagaat ctaatgctcc tgtaggctca
     5041 tctgcaagta ctaatgatgg ttttgttgct atagctctac atacagctcc tctttgcttt
     5101 tgtccaccag aaacttcgcc agggtattta tctaatatag attctatatt taattctttt
     5161 gaaatctctt ttattctatt ttctattact gatactcttt cattttttaa agaaagaggc
     5221 aacattatat tttcttttat tgtaagtgta tccaaaagat tagaatcttg aaatacaaag
     5281 cctagattat cacgtctaaa atctgccaat tcattttgct tcattttaat agtattcttt
     5341 ccatttatat aaatatctcc tgatgaaggt aaatctattg tagaaataat atttaaaagt
     5401 gttgatttac cagcaccgga agaccccata ataccaacaa attctccttt ataaaccttg
     5461 aaagttattc ctcttagagc ttcatgtttt gaaccttttc ttccatactc cttttttata
     5521 ttttcaacag ataatatttc ttccatagtt aataatcacc tttcgtcaca attagttatt
     5581 tatcaattct cacaaatata ttatatatat taaaatggct tttaaacatt taattatatt
     5641 aatttaaggt tacattttcg taagtattaa ttatatttct tcaaatttat tgcaattatg
     5701 tttaaaatat aatttatcaa gtaaaatata gttatattaa tataattatt ttaggtaata
     5761 aggtagaaat attttaattt atcaagtaga atatagttat gttaatataa ctattttgaa
     5821 ttaggaggta agaatgttta aaataatggt agttgaagat gacgtctcat tgaaaaatat
     5881 catagcaaag tgtcttacta aatggggaca tgatgttcat caaatagaaa atcttgaaaa
     5941 tataatagaa gagttcaaaa actataaccc agaattagtt ttattggaca taaaccttcc
     6001 attttatgat ggttttcatt ggtgtaatga aataagaaaa atttcaaaag ttcctataat
     6061 ttttatttct tcaagaaatt ctaatatgga tgtaataatg ggtgtaaatt taggtgcaga
     6121 tgattatata caaaaacctt tttctgtaga tgtcttagta gctaaagtta atgccctttt
     6181 aagaagaact tataattttg ttgataataa ctcaaatcag attattcaca atggtgttac
     6241 tttagattta tcaactgcaa caataaacta tgaagataat actattgagc taacaaaaaa
     6301 tgaaatcaaa attctccatg agcttatgaa atataagggg cagatagtaa gtaggaataa
     6361 actcatgaaa aaattatggg ataatgattg gtttgtagat gataatacat taactgtcaa
     6421 tgtaaataga atacgctcaa aattaaatga aattggttta gaagatttta tagaaacaaa
     6481 aagaggattg ggttatataa tcagttaaaa caattctaag taggtgaatt tatgagcttt
     6541 aaagattttc tcaaggataa actaggtttt ttagtataca attttacttt cttaattttt
     6601 acagtatcag ttattatatt ttctcctgta gatgtttttc tgactgatac tattttatat
     6661 atattagttg taaatgttgt gtttttattt ttatatctat ttattagcta catcaagaag
     6721 aacaaatttt tagataagat aaaaaatgat acattcaaaa ttaatatgaa tgatatagaa
     6781 ttagctagat gtaaaaatga agaaaaaata tacttgaaca ttattaaaaa tcatcaatat
     6841 caatgtaata acataataaa taataatatc gagaaatttg aagaaaacaa agagatttta
     6901 aatatgtggg tacatgatgt aaagatacct atagctataa taaaactcat tcttgaacaa
     6961 aatgaaaatt taattgatga aaaaatttca gatgacatag atagtgaaat atttagaatt
     7021 gaaaactccg ttgaaaaaat tttatattta tcaagactcg aagattttca taaggatttt
     7081 ttaattcaag aggtaaatat tgaaaaaatc attagaaata ttataaaaaa atactcaaaa
     7141 tattttattt caaaaaaaat tattttagac ttaaataatt tgaattttaa ggttttatct
     7201 gatgaaaaat ggctatactt catattcgag caattattga gtaattccct aaaatactcc
     7261 tcattaggag ataatatatc tatctattgc aaaagaacca aaaattattt acaattaaga
     7321 gtggaagata ctggttgtgg tataaaaaag gaagatttaa atcgaatatt caataaggga
     7381 tttacaggta ataatggaag aaacaattct aaatctacag gtcttgggct ttatttagta
     7441 aaagaacttt gtgataagct tggtcataaa attgatgtag attcagagta caaccaatat
     7501 acaaaattta ctattacatt caaatgtgat ttatagtatc ttattattga tagtttattt
     7561 tcattaactt atattcaact tcttatagta attctatatg tacatcccag tatataatta
     7621 agtagttttt ataaaaagga ggaagacatg tcttttctaa aaaaatttaa aaataaaaga
     7681 tttagaaata acagttttaa aagtaacaac tttagaaata gcagttttgg aagtagtggc
     7741 ttcaagtcta aaaataaagg ttttaaaaac aatcccttta aaaataagag attcaaaata
     7801 tatgcaggaa tagcagtaat catactttta attctaggag ttcttgctta tgttgattcg
     7861 aaaaacactg aattacaagc taatgaaaat tttatcgaaa catatactat tcctgaaaat
     7921 gaaaaaatat ttataaatgg gatggttgtt cctaagcaaa ctaaggactt caatatatct
     7981 ggagattatg agttaagtga tgttaatgtt acaaatggtc aaaaagtaaa tcaaggagac
     8041 cttcttttta ctgctaaaaa tccaactata atatcagaaa tagatagttt aaaatcacaa
     8101 cttagtcaat acaaaaaaca aaaaatatca ctttctgata ttgctgaaaa taaagatgct
     8161 attgcttcta taaatgcaca gataactgct ttaaactctc agatagcttc tctggataaa
     8221 aaagcttatg acagagttac tgctcctttt gatggcacag tatacttgaa tgaccaaact
     8281 ggaaatccag accaaccagt ttcatttatg actattcaag gtctagagtt ttatatgaaa
     8341 ggacaagcaa gtgaacaaga tttacctaaa atgaagattg accaaatggt caatatacta
     8401 gttttttcaa ctgaccaaaa gcttactggt agaataagtt ttatatctga taaaccatct
     8461 actccaaata ctgaaatggg agctcagcag aatacactat cttattatga tataaacata
     8521 gcatttgata atcaagaagg attagtaaat ggattccatc ttcaagcatc acttgaagta
     8581 gctaattcaa gctttaaaat accagcatct tgtgtactta aagacaacaa acactcatat
     8641 gtatttaagg atttagatgg tatattgaaa aagcaaattg tagatgtagc aagtcaaaat
     8701 gatgattttg ctgtagttag aggcggtctt gaacaaggcg atataataat aaaacaccct
     8761 actaaagaaa tgaaagaagg agacccagtt caaggcgatg gcgtaactgc tggttctaat
     8821 aatggagata ctactccaaa caaaaaagta gaagaagtta ctatggatgt gaattaattt
     8881 acatatgtta attaaattag aaaatattca aaaatattac aaagtaggga aagatgaatt
     8941 acatgtttta aaatccttaa atttagaaat tgaatctgga gaatttgtca tgataatggg
     9001 taagtctggt agtggtaaaa ctactttact taatatctta ggttttctag atgtatttga
     9061 tgaaggaaga tatatatttg atggaacaga tgtaactaat ttgagtgaaa atgaacgttc
     9121 tgtttttaga aatattaata ttggatttgt tttccaacaa tttaatttaa tagaaactct
     9181 aaatgtatat caaaatgtag agttgcctct aatttacaat aaagcactaa aaaaatctaa
     9241 tagagaagaa attgttaaag ataaattaag ttctgttggg cttttggaca agcttaagca
     9301 gaaaccactt caattatctg gtggtcaaca acagcgtgta gccattgcac gttgcctagc
     9361 aaatgaccct cagataatct ttgctgatga accgactggg gcattagata gtgaaactag
     9421 cagagaaatt atggagcttt taacaaggct caacaaacaa ggtaagacta taataatggt
     9481 tacacatgat caagatttaa ctaagtatgc aactaaggtt atacgactta aagatggtgt
     9541 gtttacttcg gaggtgtaat catgagttta ttaaaaaatt ctttggccaa tttaaaaggt
     9601 cacaagcttc gtgtatttgt agctttactc tggattataa taggaataac ttcagtaata
     9661 cttgttagtt ctataggtaa tggatttcaa aaagaaatta agaaatctgt aaacaatgta
     9721 aaccctaata aaactactat ttcgtttgaa tcagccgata acacaggtct tactgatgat
     9781 atgagtattt ttttaaaacc ctttaatgca aaagatttag aagaattatc atttgttgaa
     9841 ggagtagaaa gaatagctcc ttcaagagat gggtttaacc ttgattcagt ttactcttca
     9901 caagcatctt ttgataaaaa aactacgtat gttgatgttg gaccagtaaa aaaagattct
     9961 aaaataaatt taatttgtgg tagagatttt tctttagatg atgaaaaaag aaaagtaatt
    10021 ttacttacat tacaaagtac aagtgaaatt tttgaaaatc cagaagatgc cttaggtcat
    10081 ggtatcaaca taaatggaac tatatttgaa ataatagggg tattagatga ttctcagcaa
    10141 aaccaagctg gaggattctt tggaggttac caagatatgc aatttactac ttccctagta
    10201 cctaaaaagg cttttgacac tttaatgagt caaaattctt attcaaatga aatttaccag
    10261 ttagacttag tttcttctaa aggctataat gtaaatgaag ttgcaaacaa tgttatagct
    10321 aaattatatg agatgcaccc tggtatcaat ggttcttata ctacaccaga cccaactgaa
    10381 cagacagctt atcttgaaag cattaattcc aatgtaaaca aatatgtatc tataataact
    10441 gtagttgcaa tgtttgttgg tggtattggt gtaatgaata taatgtatgt atctgttatg
    10501 gaaagacaaa gagaaattgg tataagacgt gctattggag caaaacctag gtctatatta
    10561 tttcaatttt tagttgaagc tgtttttata acagtatgtg gtggaatttt aggaactata
    10621 gttggattta ctgctactaa ctatgtttct aaatatatag gttttgaagc tatacctagc
    10681 ttaaatagct tattttatgc aattgtagct actatattga ctggagttgt atttggtctg
    10741 attccagcct ttaaagcctc taaactagac cctataaagg caatttataa gtagaaattg
    10801 tataaagatt ttcgattttt agattagttt atataaaaca tacttaaata tataacgcac
    10861 tattatatat gatagattag gaacttaatc tttccaaata taatagtgcg ttctttcata
    10921 aaaaggattc tcttccttac tatatctaaa ttaccaagat gttatgttct aattgttcaa
    10981 actaatactt ccaattttga ttaatcaatc tcattctcct aatatacaat cttttacttt
    11041 caatcattgt acactccgcc ttaaaaaaat aatcttaaat taggcgttat atacaatctc
    11101 cccattacat attgtataat taacctttcc atataaagat ttacctttaa atggtgaatt
    11161 atcagcttta gaaacaaagc tttcaactgt ccattcttct tctggattga atataacaat
    11221 gtctgctaca gccccttctt ctataaatcc aatatttaaa ttgtacaact ttgcaggatt
    11281 tatagacatt ttttctatta actgcatcat actaagatga tttttatgca ccaaatttgt
    11341 tattccaaga gctaaagaag tttcaagccc aataattcca cttggagctt ttataaactc
    11401 tctatctttt tcttctttac tatgaggtgc atggtccgtt gctattattt caatagtacc
    11461 atcatttaat ccctctataa tcttatctct gtcccattta cttcttagag gtggattcat
    11521 ttttgcattt gttccaaact ccaatacatc ttcctcagtc aatgtaaagt gatgtggtga
    11581 agcttctgca actacatttg cacctaagta ttttgcaaat ctaaccatat caacagatac
    11641 acctgaactt atatgttgta tatcaacttt agcaccagtc tttagtgcca acatacaatc
    11701 tctggctacc ataacatctt ctgctacact tgatgcacct tttaatccta gttctgaagc
    11761 tacctttcct gcatttacac ctggatttcc aactaaagaa gggtcttctt catgaaaact
    11821 aagtggtaca tccaactctt ttgccttaat cattgcctcc ataattagat tgctatccat
    11881 taaaggtaat ccatcatcag taaaccctgg taccccagct tttttaagag cttccatatc
    11941 aactaactct ttaccttcaa aacctttagt tatagctgct gcttgtaaaa catttataca
    12001 tgcatttttt gacttttctt taacataatt aaaagtatct tcattatcta caataggatt
    12061 agtatttgcc atacatataa cagttgtaaa tccacctcta gcagcagatt tagctccact
    12121 ttctatatct tctttatatg taaacccagg gtctctaaag tgtacatgta catccactaa
    12181 acctggagca actacattac cactagcatc tattattttt tcatattcat cactttcatc
    12241 aaacttgcct attttataaa ttttattttc ttttataatt aagtcaatat tctcatctct
    12301 ttgactttta gggtctatta ctctaccatt tttaattaaa atcatattat ttctccttta
    12361 tatttttaat ttatatagac tcatataata ttatacatta ttttacatac ttaaatacaa
    12421 aattacgttt gacatcaata gcctttttag gacaaagttc atggcaacag taacatctta
    12481 tacatttact taagtctata atagcaccct tagtccctat ctctatagtg gatgcaggac
    12541 aagcttcctt gcattttcca cattttatgc aatcttgaaa tcttaccaca ggctttggtg
    12601 ttataagctt tgaaataggt tcatttaaaa attttggtag ctttacagaa aacaatctta
    12661 aatccttact tacatttgga atcttgtaat tttttataat taaatctttt atatcttcac
    12721 ctaaaatttc aatatctgaa aaatcttctt gtatatatcc tctcttaata gcacctctaa
    12781 ttgtagcaac tctatttgga agtaaactta taatcttaca tgcaacaaca tctatagcat
    12841 atggattttg tgatgctaat aaaactccta tttctcgagt agtaccagct gaaggtccat
    12901 caccttccat acctactata ccatccataa ttgtcaatgt tggttttata taatcacaaa
    12961 tatctaaaag tacattttcg caaaacaact cttctgtagg aaatctataa tgtatctctg
    13021 cttttttcaa tccagctatg catccatata agttttttgt accaccagta aaagttgcca
    13081 ttatatgagt ctttaattta caaagattta ttatataatc tgaatctact attggtttaa
    13141 taatatccat atattttaga acatgtgctt tttcactatg tatcttaaca tctgaaacat
    13201 cataatttaa cttaatgccc agttcatttg ctacttcttc aattccagat gccttataaa
    13261 tgctcttaag agaactttga ttatatggac caccagggct atctgctatt gtaatatcac
    13321 aatttaactc taaaagtttt tcacatacaa cttttacaac cataggatgt gtagttgtag
    13381 cttcttcagg cttttttttc atcaataaat ttggttttat aagtactttg cttccttttt
    13441 taacaaactt atctatacca ccaatatcct ctaaattttt ttcaatagat aactttactt
    13501 cttcataatt gtaagtttta caactcctaa cagataccga cttcatatta tctccatcct
    13561 tctatcacaa cagtttattt gtataattta aaaaaggaga aaccaaagtt tctcctttta
    13621 atctatgttt taattattat tttccaaaat gtctgtttaa taatcctaag aatgctttac
    13681 catgtctagc ttcgtcttta cacatttcat gtactgtatc atgtatagca tctaatccta
    13741 attctttagc tctcttagct aattttaatt ttccatcagt tgcaccatac tcagcatcaa
    13801 ctctaactct taagttttct tttgtatctg caaccacaac ttctccaaga agttctgcaa
    13861 atttagcagc atgttcagcc tcttcaaaag ctattctctt atacgcttca gctacttctg
    13921 gataaccttc tctatcagct tgtctactca ttgctaaata cattcctact tctgtacact
    13981 caccagtaaa gttagctctt aatccttcga ttatttctgc atctacacct tgagctactc
    14041 ctattctatg ttcatcagcc caaaccattt cgcctttcat ttcttcaaat ttatcagctc
    14101 caactttaca cactggacat tgtgctggtg cagcatctcc ttcatgtata tatccacata
    14161 ctgtacaaac aaattttttc ataattaatt tcctcccaat tttatataat taaattttta
    14221 tttttaagtt taataacttg ctacaagaat atatataccc cagactttat agcttaaaca
    14281 ctaaaaataa aatttatttc ctaattaatt ttatcacttt gaaacaccag tatcaaccaa
    14341 gttttgatgt caaatttcta cataatacat aatttatttg attaattagg aaatagttgt
    14401 catattaaat actttctaat gaagactcct ctgaatcttt aagtttcact tttttgctct
    14461 ttaaaaaaga taatttagtt tctgcagtta tgattgcaac aaatatcaag atactaccaa
    14521 taaccaattt tgctgtaata agttccttta gaattattat agaaaatatt gtaccaaata
    14581 cagcctctgt ggataatatt atagctgttc tagttccatc aactcttttt tgacatatag
    14641 tctgaaataa aaatccaatt gtagtactaa atacagctag atacatagtt cccatgtaac
    14701 cagataattc agcttccatc tttaattgac ctgcaaatgt ttgaactaca acagacatta
    14761 aaaacgctac tgtaaattga atagctgtca atgccattgg gttgttatct tttgcaaact
    14821 cacttgtaaa gaatatatga aatgcaaatc cgaatgaaca tataagtgtt agaaaatctc
    14881 caaaattaat agagaaatca gcttctaaag aaagtatccc aattcctata agagctacca
    14941 aactacttat tattcctatt ttatcaagtt ttcttctata aagtataaat cctatgaacg
    15001 gaactattac aacattagct gctgttataa acgcattttt agatggtgta gtgtacataa
    15061 gtccaatagt ttgtactgtg aatgctagaa ataaaaatat tcctaaaatc cctcctgctt
    15121 ttaataattt tttacccata ttggcacgaa tctgtttaaa aaacagtaaa ttcataatta
    15181 tagctgccaa gaaaaatctt aaagttatga tttgtagagg tgtaagccca ccgtctaatg
    15241 caaattgggt tgctacaaag ccacttcccc atataattgc tataaatatt agtcctattt
    15301 ctccaaagta ttttttcaat aaagccccct cctttttatt tttttgcaaa attataaacc
    15361 ccctaaacag caagagagtt tttctataag cattactata aattaatata gtaaattttt
    15421 tcagtatttt aaatctaaat ttacatacat tttgagctca aatttatatc cactatttaa
    15481 cagtaatata ttaatcattt tttgtaaaca cctttttaat aaatttttat attttttata
    15541 tttttttacc tatactaaat ttttttatag atatttttaa tatatatgat actttaatat
    15601 tatgcaaata atgtcatata agttacactt tagttatctt agttacaact ttgtcggttt
    15661 actttttcta ctttcttcga tataattata atatattttt gatacaattc gacaaaaggg
    15721 ggaatgctgt aattatggta aaaaaaagaa aaaagaaaac tgttacactt actatacttg
    15781 ttgtaatagt tattggtatt ttttctatgg catgggcgga catgtctaga aaaaaagaaa
    15841 tgaaagaaac tcaagaaaga cttaaagctg aaagattaga aaaagaaaga attgaaaaag
    15901 aaaaaaatga acctattatt ggtgcaaaaa aagaagctgt aaaagaatat ggatatgacg
    15961 caaaaatagt ttgggacaaa cttaacaaat acgactattc aaacaatgga gaaaagatag
    16021 tctttcttac atttgatgat gggccatcta ctactaatac tccacaagtt ctagatatct
    16081 tgaaaagaca tggtgttaga ggaactttct ttattaaggg tgattctctt gaaagaaaag
    16141 gagctaatga aattttaaaa agaacatttg acgaaggaaa tgcaatagcc catcactctt
    16201 atactcatga ctataaaaaa ctatatccta acagaagcct aaatctcgat acattcgtaa
    16261 atgaacttaa caaaactgat gaagctatga aaaaagtctt aggaaagaat ttttcctcaa
    16321 atgtagttag atgcccaggc gggtatatgt catggaaaaa tatggaacct ctaggaaact
    16381 acttaaaaga gaaaaacatg gcatcaattg attggaacgc tttaaatgct gatgcagaag
    16441 gtaaaaagaa aaatgcacaa gaattatttg aacatgcaaa gaaatcttct gaaggcaaag
    16501 aaatggttgt tcttttaatg catgatactt atggaaaaca agaaacagta aatgcattgg
    16561 accaaattat aacatatttt aaagacaatg gttatcaatt taaaatttta atataataaa
    16621 aaagcaagtt tattgaatgt acattttata ctcaatagac ttgcttttac tttactctat
    16681 tttactagct cgatttttac aaattttttc tttcctattt gaacaactaa ttcactctct
    16741 tttacaatag tgcttaaatc ctctactttt tcaccattta cctttactcc accttgagaa
    16801 gctaatcttc taacttcact cttactctta acaatctcat tagaaactag tacttcgatt
    16861 aagtcaaatc catcttcctt aacttgaatt gtttgtatat cttcaggtat ttgacctttt
    16921 tggaatacag atttaaatct ttcttcagct tctttagcag attcttctcc atggtataat
    16981 gttactattt ctcttgctaa gttcatcttt atatctcttg gatttactga accctctttc
    17041 aactgagatt ctattttatt aacttcatca ggatgaacat ctgtcactaa attgtagtat
    17101 ttaattataa gttcatcagg aatttccata gatttttggt acattattcc cgcttcttca
    17161 tctattccaa tatagttacc taaactctta ctcatttttt ctttaccatc taatccttct
    17221 attaaaggca tcattattac tatttgagac tccataccaa attctctttg taatgatctt
    17281 cccattagta aattgaatct ttggtcagtt ccaccaagtt ctatatcagc ttctaatgct
    17341 atagagtcat atgcttgcat taatgggtag aagaactcat gtactgatat tggcatttgt
    17401 ccttcatatc tctttttaaa atcttctctt tctaacattc tcgcaacagt aatagttgct
    17461 gctaatttta ttacatcttc aaaatttaac ttagctaacc actcgctatt aaatcttaca
    17521 atagtttttt ctttatctaa aactttgaaa atttgttctt cataagtttt tgcatttgca
    17581 agaacttgct ctgttgttaa agcttttcta gctttagatt ttccagttgg gtctccaatt
    17641 tttcctgtaa aatctccaat tattattact atttgatgac ctaaatcttg taattgtttc
    17701 atctttctaa gaactactgt atgccctaaa tgtatatctg gagcacttgg gtctagaccc
    17761 aattttacta tcataggctt tccttctttt tctgatttaa taagcttttc ccttaactct
    17821 ttctcatcta ttaaatcatc tacacctttc ataattattc gcatttgctc gtctatcgat
    17881 ttcatttttc attcctccta aaatatttat ataatctata atttttattt ttataataaa
    17941 aaaagctttc atcctcttaa aaggacgaaa gcattgctta cgtgttacca ccttagttca
    18001 tcaatatctc acgatattaa tctcttagag tacaattaca tatactcgtg tactataacg
    18061 tgtactaatc cgatatctct taatggttgt tatctttcag agatagtgct ctgagatgta
    18121 ttcaaactaa atcaatttat tccatttcac caaactggaa ctctctgtta aataaaatat
    18181 agccctactt cttctcttca tagcatttat atattcagat tatttcatca attacaagaa
    18241 atttttctta acttactcat aatatattat tatttttata ttttgtcaac cttttttatt
    18301 attatataca tgttttttta cattatacta cttttttata ttttttacca atacccaaaa
    18361 tttatttttc aatcaaaaga tttatttgtt tattgactat attactctga ctcaatctta
    18421 tatttatttt cttatttaaa aatgcgttta taattattaa actaataact gttacatcaa
    18481 atataaaaaa cagcaataaa attattatca aaaataaaat tctagcatat aataattttc
    18541 taaacttagc tattataaag ttttgtagtg acatacatga gtctctaatg ataaaataga
    18601 cacttatata ggttattgat ataaagattg tttttaaata aacagaatca actttaacat
    18661 tcaacagttt tagaacttca aaaatggcta gataagaaac acatatgtaa aaagcttttt
    18721 ttgagcaaca taaaaattgg tttaaatttc taaagttctt aaatattaaa aattttttta
    18781 aagaattaga atctaaaata tgcaatttat tccctaaaat taaacttaat aaatatatgc
    18841 aaaacaccat accaaaagga aacaatgcta tcattaaata ataaataaaa tttgtatacc
    18901 cattaaaata aatccatttg ctatatcctt gaaattcttt cataaaacta taacctatta
    18961 atgttgaagc atatatatca atgaaaacca ttacaataac cccaaatact gaaccatata
    19021 ctaaatcatc cataaccatt gtaggcttat ttattagata gccacacatt ataccaagta
    19081 atacactctg acttgcccaa atagcagttc ctagatttcc aagtaccaga gaaattatta
    19141 aaagcgaagt aattccagat aaaatggtat actttaaatc acatttcaag cagattatac
    19201 aaaaaaatac tggcactata aaatctaagt aaatagcata tccaaatcca gtactcacag
    19261 ctataattgt tactactatg aataaagatg acaataaagc tgcttcagtt aattttttag
    19321 tagtattcaa aacactgcct ccataaatca taaattttct aactaaagtt ttgtttttaa
    19381 tatagtatat cactttttaa aaatattttt aataaatata aattttaata aaggtattta
    19441 tgttaaaaat ggtataatgt atcttaatat tcagtttttt aagatgctag gaggtaatta
    19501 ctatgtttaa aattaaacac tttaatgatt tgagtttaga tgaattttat gaaatagcaa
    19561 aatcaagata tgaggtattt gcttgtgaac agaagatatt ttcattaaac gattatgatg
    19621 atatagataa aagctcttat cacatatttt taaaagaaaa tggattgatt tgtgcttacg
    19681 ctagaataat tcctaaagaa tacagctctt ataatgatgt ttacattggt agagttttag
    19741 ttctttctag tcatagaaga aaaggattag caaaacaaat gatggattgt gctatagatt
    19801 ttataaaagt taacttacac gaaaataata taaccttatc agcacaaact tatataaaga
    19861 acctttactt atcttgtggt tttaaagaaa tctcagaagt atacgacgaa gcaggtattg
    19921 aacatattaa aatgagatta tagtgttgac aagctaatat tgttaatata taatttaagt
    19981 ataaattaaa tattgaattg atatttaggt atttgaaaat accttacata gagtcagaag
    20041 gcttttgctt tctgactttt ttattatcaa aattcaataa ttatatggat tcaaagtgtt
    20101 aatttttatt ctatattcta ctttccccaa aagtaagaat tatacccaac tttgtatgat
    20161 aggcatcttg cctatctttt tttgtaaaag taacaaggta ataatataat gcatagttta
    20221 aaataagaag attagcccag gctaaatttc aagcatggag gtgctattat gaaaaacctt
    20281 aacgatattc aagtgaaaaa aactgtcaaa gttgaagata tattatctag tggtaattta
    20341 agagaaagaa tgttagcgct tggtcttact agaggagctg ttatagatgt agttagaaaa
    20401 ggtccaaaaa ataatttaac tgtctataaa atcagaggta gcaagatagc tctacgacaa
    20461 gaagaaagta gcttaatatt agtttctgac atttaaaaat ttctagcagg aggatattaa
    20521 tatggggttg acacataact caactaaaat gagttcttta aaagacatgt ttgatataga
    20581 caataaggaa aaccagtttg taattgcatt ggctggcaat ccaaatacag gtaaaagtac
    20641 tgtatttaat catcttacag ggcttagaca gcatactgga aactggccag gaaaaactgt
    20701 agctacagct cgtggtaatt ttaaatataa aaataccgaa tatgcattaa tagatttacc
    20761 aggaacatat tctttatttg ccttatcaca agaagaaatt gtagctcgtg attttatatg
    20821 ttttggtaat ccagatgcag taatagtagt atgtgatgct acatgtcttg aaagaaattt
    20881 aaatttagta tttcaagtga tggaacttac agataaagtt attttgtgta ttaatttaat
    20941 agatgaagct agaaaaaaag gcattactat tgataaaaaa cttttagagg attctcttgg
    21001 tattccagtt attttaactg ctgcaagaaa tggctctggt atggatgaac tattggatac
    21061 tttaaatgat gtatcttttg acaaatataa attaaataat aaacctgtta gatacaatga
    21121 aaatatagag aatgtggtaa aatcaattca accagaattg gataatatta taccaggtat
    21181 aaattctagg tggttgggac ttagacttat tgatggcgat gaaagtatat ttgaatctat
    21241 gagtaattat atagacaaag attctataga tgctattaat gaagtaaaga aaaagattcc
    21301 agacaatata aataaacaga aaataagaga tgaatttact aaaatcaact atgattatgc
    21361 taaaaaactg agtgatgagt gttgttctaa tgttgctaaa aaatcaactg atagagaaga
    21421 aaaagtagat aaaatattaa catctaaaat atttgggctt ccaataatgc tcttattatt
    21481 aggtactata ttgtggatta ctatagaagg agctaactat ccatccactt tattatctaa
    21541 tttattactc ggatttgagc caagtatttc gggtatatta aatagtatta attgtccatc
    21601 atggttaaat gatatgttgg ttttaggatt atacagaaca ttagcatggg taatctctgt
    21661 aatgctacct cctatggcaa tattttttcc attatttacc ttacttgagg attttggata
    21721 tttgccaaga gttgcattta acttagacca cctatttaaa aaagcttgtg cacatggaaa
    21781 acagtgtttg actatgtgta tgggatttgg atgtaatgca gctggagtta ttggttgtag
    21841 gataattgac tctccaaggg aaagacttat agctatactt acaaacaact ttgttccttg
    21901 taatggtcgt tttcctactt tgatagctat atctacaata ttttttagct cagttataac
    21961 caactctttc gtatcaagtg tagcaactgc actttgcata actttactga taattctagg
    22021 tgttataata acattgttgg tttcttacac tctttctaag acgctattaa aaggagttcc
    22081 gtctacattt acattagaac ttcctcctta tagagttcct caaataggaa gaactttata
    22141 tacctctata atagatagaa caatatttgt tttaggaagg gctgtaatgg tagccatacc
    22201 tgctggtgtc ataacttgga tatttgcaaa tatatatata ggagatttaa gtatattatc
    22261 acatgttgca aactttttag accctttagc taaattaatt ggtcttgatg gttttatatt
    22321 acttgctttt attcttggat tcccagcaaa tgagatagtt gtacctatac ttttaatggc
    22381 ttacctagca actggttcta tgatagaatt agatagcttt agtgctttag gtcaagttct
    22441 tagagaacat ggatggactt atttgacagc tttaaatgtt atgctgttca gtttactgca
    22501 ttggccttgt gcaacaacac ttttaacaat aaaaaaagaa actggtagtt taaaatggac
    22561 tgccttagga ttcttaatgc ctactatact tgcttttgtt gtttgttttt taacaactac
    22621 agtttacaat ttatttatat aattagtatt aactgacagt agacatatat tagagaaact
    22681 atttaaaaat ttatatactt tattataaag catagagata tattcaatga taagtaaaat
    22741 tacttaacat ttgaatatat ctctatttta attcttatct ggatacttgg attgaaattt
    22801 cagtcatata attatcttca ttactttcac aaaaaaagtc aagtatactc tcctcatacg
    22861 aatttccaat aattttatat tcattttcat atatatattt tttcaatttt tcgtaagatt
    22921 tatatgaatc ctcatatttt ccaatatgtt ttatgcaagc atacattcct tctggcttta
    22981 catatagctt tttatccttt atttttttat ctatcttcaa ataaaaactt gatatactta
    23041 aatagtcccc attttttata ttttcattac ttactattac tcccaactca tatccttgat
    23101 acaaattttt cgatttacaa taattggtaa aatctattaa ttccatcata aaatcactat
    23161 tactcatatt actcaaatcg cttgatagaa ctaaatattc ttcaaacttt ttggatatat
    23221 aaatttcttc actattcatt ttagaaattc cttcaactgt tatatttatt ctattttgca
    23281 tcttagaact tatctttctt agttttttta ttttttctct tatctttttt tcttcttctt
    23341 taagcatttt cacacaaagc tggggagttc tattttgtat atactctttt atttcctcta
    23401 aactcattcc tatttctctt aatgattgta atatatcaag tgtatcatat tgataaactg
    23461 agtaatatct ataattattt ttatctttat attcaggaga aaaaatacct attttgtcat
    23521 agaaaattaa tgtctgctta gatataccac acaatttagc aaattcacct gttgtaaaat
    23581 actgtttttc caagggttat acactccttt tctttttgtt gtctatatag ttactatatt
    23641 ctttattatt tactaaatca agtagttatt attttagaaa atcaatatgg ttttgatttt
    23701 aaagcttaaa tatcagttag atgtcagtta taaatatttt acccattccc atcataaaaa
    23761 aagatatacc ctcttaactt ttggatggaa tattatattt atgcttgtta gagtgaaaat
    23821 tgtaataggt gctacaataa gatatttaat taaacataaa gttaattatt gtaataaaaa
    23881 atctttagat aaattagagt taaagtatgg aaatatagat agagaaaaag ctgttaaact
    23941 agaaatattt tattaatatt taatagaact tgaatatata gaaatgagtt tattaatagg
    24001 aagattagat actgcaataa catcattagc attagtatct attataacaa tagcatcata
    24061 ttatttaatc agaaaaaatg tgtaattatg taagctattt ataataaaac actgataaaa
    24121 ttaataataa aaaggtaata ggatttctat actacctttt ggtttttata atatagtgta
    24181 attttattat atttttgtat gctaatataa ctagataatc ataagataac acattaaaag
    24241 aaaattatat tctgtatagt tagtataaaa cgtaagtaat ataaatttat gtaaagcaac
    24301 aaaagatgga tttacattcc atatagttaa tataaaacgc ttatgacctt ccctatatcc
    24361 ctggttctta tgatatttac attccatata gttaatataa aacaaagatt tgatttttgt
    24421 ttctgtttat ttatttataa tttacattcc atataattaa tataaaactt aataaagaca
    24481 tatttgttat tttcaaatca ttttgattta cattccatat agttaatata aaacctctta
    24541 taagtatgac tcctatacaa aaatttacga tatttacatt ccatatagtt aatataaaac
    24601 taaagataga ataaagaaag tatgcttttc aaagaaattt acattccata tagttaatat
    24661 aaaacacaca gttgatgaaa cagaaaaaga ggttcttttg gaatttacat tccatatagt
    24721 taatataaaa caatgatata aaagacgttt ttggcgaaaa agttatagat ttacattcca
    24781 tatagttaat ataaaacgaa atctttattc agcgattaca agaggagata ttgcatttac
    24841 attccatata gttaatataa aactaatata ctttgttact attccatctt taaatccaca
    24901 atttacattc catatagtta atataaaacc aaaattaaaa ttttttagtg acctattctt
    24961 taaaaaattt acattccata tagttaatat aaaaccagaa taataaaaga agcgaaaata
    25021 tattagactc gtatttacat tccatatagt taatataaaa caagttgtag aggaactagg
    25081 ttatccgatt tttttgcaat ttacattcca tatagttaat ataaaacacg acagcagaaa
    25141 accaaattga tttatacgtg tgggaattta cattccatat agttaatata aaaccggatt
    25201 ttaaacctcc ttttgatgtt cctgttagta aatttacatt ccatatagtt aatataaaac
    25261 ttgcctgttc gagaagtagt taatactttc gtgattattt acattccata tagttaatat
    25321 aaaaccacat aatacttctc aaattcctaa taattgttat ttatttacat tccatatagt
    25381 taatataaaa ccttgacgaa actaaactag tcatacccgt tcaaaacatt tacattccat
    25441 atagttaata taaaacttct agtcctgtat ctataaaaac aatctcgtct aaatttacat
    25501 tccatatagt taatataaaa caaaagcaaa aagccactct cgtgcaaaga gtgacttaca
    25561 tttacattcc atatagttaa tataaaacaa tatgagctta tgaaagagct tgaaatgaat
    25621 atttaattta cattccatat agttaatata aaacaaaaat caatacctat aataaaaaag
    25681 ttttgagttt atttacattc catatagtta atataaaacg caaaatcaaa aatttgactt
    25741 aataacacca ttaattaatt tacattccat atagttaata taaaacggtg atacttttgg
    25801 gtaagcttct ttcacctagt tggatttaca ttccatatag ttaatataaa acctttaact
    25861 gtatatactt tgttgtcata ggcagtacca tttacattcc atatagttaa tataaaacga
    25921 agtgttggag aatataaaaa tttatattta aaagttcatt tacattccat atagttaata
    25981 taaaacctga gctgatagga gctgtgtttg gaggtgtaaa aaaatttaca ttccatatag
    26041 ttaatataaa actggtggag ctactgatat tagacttatt ggtggtaaca tttacattcc
    26101 atatagttaa tataaaaccc caaaataaac ttagtatttt caacacttac acgtatacaa
    26161 ctctcttaaa tttgcagtga acgagcaata gtgcaattga taacgcttat catgcactct
    26221 caacgcctta tatttcaact gttacgccaa attttttcac aaatatcgct cactgcaaaa
    26281 ttcctataat tttattatat cataaatata ttacttttga atattgatac caatttatac
    26341 cataataaaa gcaaaaaaac gtaatctatc tacattagat ttaaattcta taacttaaag
    26401 ttttttacat aatatttatg cttgtttaat caatctaata tatcattctt gtatattgct
    26461 tttaatagtc ttttttctta aataaatatc tatctgaaca agcatagttt aattaaaaac
    26521 ttttgtattc ccttaaaact gtatctacct cttattattt tatttatgat atattatttt
    26581 taatctaaaa atagatatct atttttatta tggttatccc tttttaaaat actttaatca
    26641 tatactattc ccaatttaac atccattgaa atgctatttt cttatattgc aatttataat
    26701 tgatatatct tcaatataat aatttaatgt atttttgatt ttaaagcttt tcagtgatat
    26761 tattgataaa ttagaattaa ttatctagct ttaaaaggtc aaagatttct gttgctgtct
    26821 gcggttttcc aaataaatat ccttgtatgt aatctggctt tatttgttta actaaatcat
    26881 actcttcttg tgtttctaca ccttctaaac atacttttat tcctacgtca tgacaaattg
    26941 caacaataaa atgtataaat gtagcatcaa acttactctt taatatatct ttaacaaata
    27001 ctctatcaat tttcactata tctattggag aaaattttaa aacttctaga gacgagtatc
    27061 ccgtaccaaa atcatccata gcaatataaa ttcctaaatc ttgtaaagcc ttaaatttaa
    27121 actgtaaaat actcatattt tgtactgtat gactttctgt caattctaaa acaagatttt
    27181 gataaggaaa attttcatca tctataattt ttactatatc ttcaataaat gtgtcttcta
    27241 atatttgaac agctgaaacg tttacactca ctttaaaaaa aggatagtaa tcaatccatt
    27301 tcctaaaagt acgtaatgcc attcgaagca cccaagcccc tacaatattt atcatatcat
    27361 tttcttctaa tattggaata aattccaaag gagaaattgc tttgcatttg tcattagtcc
    27421 accttaaaag aacttctaca cctattattt tatgactttc tgtatctact tgtggctgga
    27481 atcgaagact aaacccccta aaatcatcat taattgatgc ctttaaatcc cgcatcattt
    27541 ctaatgaata cattttattt tttaatattt cttgggagaa aaaaacaagc ctgtttttac
    27601 catgctcttt cgcatattgt aaagagtaac tagcacactt ataaagttct tttactgtat
    27661 ctccatgttt tggatatatt acacaaccag cagaaattgt tatatttaat ccatattttc
    27721 tccatagttg aaggtgtata atcatattct gtatttgatt atatagagtt agaatttcat
    27781 tttcttcaac attatcaaca agaataccca tttcgtcccc atcaagttta taaagttcag
    27841 cattatctgg taaaatagat tgtatagact gtgctagtgt ttttattata ttatctccaa
    27901 aatccctatc atacatctca ttaacatttt taaaatcgtc aatatttaat aatactatgc
    27961 ataatttttc aatcatagga tttgaaattt tatcttcaaa tacactcata aattcatgat
    28021 aattcagtaa ctgtgtcaat gaatcaatct tattttgttt acctaattgc gtcattattc
    28081 ctgcaaaaat acttggttct ccaaattcat cacgcattag ttgacctcta catcttaacc
    28141 atatatattc tccactacga tttttagccc taaattccac tgtatgataa tccatctggt
    28201 ttttacctat ttcagtgttt gacttataaa aacgattcca gtcttctgga tgtacaattt
    28261 ttttccagta aataagcgga ttttcaacaa tttctcctgg taagtcaaat aattcaactt
    28321 gtgatggtga atatcgaaat acgcctgttt tcatattgca aatgtatata tattcatctg
    28381 tacctctaat caaagcatcg taaagcaatg tagaattata atcaaaattg tctcttgaaa
    28441 aattaaaaac tcctttttct tctttattat ttgatatttc atgaaattca ttaatcttaa
    28501 ttggtttatt taagcttgtt gagtttgcaa tatctattga ttgatgcata tgaacaactg
    28561 tgccatcttg ccatgtaatt aaactatcat aattttcaaa aacacgattt aacttattac
    28621 atttttcata ccactttatt aatactcctt ttttatcatt ctttaataat tctaaaactt
    28681 tacaaaaact acaagtagaa tttttttcag gatataatac ttgccaacaa acttttcctt
    28741 ctggatctaa aatattatat tcttctttca tttttttgtt cataaaaata atttcattag
    28801 tatgaatatt tgtaacatat atattaatct gtagttgatt taatataact tcaaaattat
    28861 gtttgttcat atacttatct cctccatagt tactgctctt tataacaaca ttgttaaaat
    28921 tattcttttt agaagattta cttataactt atacatatac actaaatacc ttaatttaat
    28981 tagaaagttt attacttcat attttaaatt ataattgata aaaaaaaata cagcaatata
    29041 gtattaccaa tttaaaaaaa attaatatta cataatacat ctctc
//
LOCUS       DAMJYC010000048        28606 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJYC010000048 DAMJYC010000000
VERSION     DAMJYC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.049"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            128..478
                     /locus_tag="PC472_002208"
     CDS             128..478
                     /locus_tag="PC472_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2727530.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            497..2140
                     /locus_tag="PC472_002209"
     CDS             497..2140
                     /locus_tag="PC472_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2727531.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2221..4278
                     /gene="recG"
                     /locus_tag="PC472_002210"
     CDS             2221..4278
                     /gene="recG"
                     /locus_tag="PC472_002210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2727532.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4337..4891
                     /gene="rsmD"
                     /locus_tag="PC472_002211"
     CDS             4337..4891
                     /gene="rsmD"
                     /locus_tag="PC472_002211"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2727533.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4893..5390
                     /gene="coaD"
                     /locus_tag="PC472_002212"
     CDS             4893..5390
                     /gene="coaD"
                     /locus_tag="PC472_002212"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2727534.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5394..5918
                     /locus_tag="PC472_002213"
     CDS             5394..5918
                     /locus_tag="PC472_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727535.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6262..8181
                     /locus_tag="PC472_002214"
     CDS             6262..8181
                     /locus_tag="PC472_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2727536.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8194..8481
                     /locus_tag="PC472_002215"
     CDS             8194..8481
                     /locus_tag="PC472_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727537.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8614..9873
                     /locus_tag="PC472_002216"
     CDS             8614..9873
                     /locus_tag="PC472_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2727538.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9876..10616
                     /locus_tag="PC472_002217"
     CDS             9876..10616
                     /locus_tag="PC472_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2727539.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10939..12249
                     /locus_tag="PC472_002218"
     CDS             10939..12249
                     /locus_tag="PC472_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2727540.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12347..13264
                     /locus_tag="PC472_002219"
     CDS             12347..13264
                     /locus_tag="PC472_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2727541.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13280..14089
                     /locus_tag="PC472_002220"
     CDS             13280..14089
                     /locus_tag="PC472_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2727542.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14147..15895
                     /locus_tag="PC472_002221"
     CDS             14147..15895
                     /locus_tag="PC472_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2727543.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16157..17179
                     /locus_tag="PC472_002222"
     CDS             16157..17179
                     /locus_tag="PC472_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2727544.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17184..19490
                     /locus_tag="PC472_002223"
     CDS             17184..19490
                     /locus_tag="PC472_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2727545.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19462..20577
                     /locus_tag="PC472_002224"
     CDS             19462..20577
                     /locus_tag="PC472_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727546.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20595..22013
                     /locus_tag="PC472_002225"
     CDS             20595..22013
                     /locus_tag="PC472_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2727547.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22028..23236
                     /locus_tag="PC472_002226"
     CDS             22028..23236
                     /locus_tag="PC472_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727548.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23310..24698)
                     /locus_tag="PC472_002227"
     CDS             complement(23310..24698)
                     /locus_tag="PC472_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2727549.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25039..26373
                     /locus_tag="PC472_002228"
     CDS             25039..26373
                     /locus_tag="PC472_002228"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2727550.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPSIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNISK"
     gene            complement(26515..27432)
                     /locus_tag="PC472_002229"
     CDS             complement(26515..27432)
                     /locus_tag="PC472_002229"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2727551.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27640..28524
                     /locus_tag="PC472_002230"
     CDS             27640..28524
                     /locus_tag="PC472_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2727552.1"
                     /translation="MKVLKKITILLLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        11160 a         3069 c         5108 g         9269 t
ORIGIN      
        1 ttctaggttt aaattatacc atatttatag tatgatatga ttataatttt atataataaa
       61 taagtaaaaa tagtaaaaaa taaattaaga aatgttttta ataaaaataa gcttaagggg
      121 gtcaaccatg agtgcaaagg taacgaatca atatggaaat atagaaatag ataatcaagt
      181 aatagctcaa gtcgtatata gagctgcaat ggaaagttat ggcttagtag gtttagcata
      241 taagtctaaa ggaatagtag agctattaaa aggcgagaac gcaactaaag gtgtaagagt
      301 tgaggaagta gaagaagatg ccatagcaat cgaactttat gttataatac aatatggtac
      361 aaatatatct actgttgcca ataatataat agatagagta aaatatgtag ttgaaaaaat
      421 gactggcgta agagttacta gaattgatat taatgtacaa ggaataagag ttaagtaatt
      481 tcataggagg aaacaaatga ttcaatatat agatggtaaa aggttaaggg agatgtttat
      541 ttcaggtgca aacaatctcc aaaacaataa agagttagtt gacaagttaa atgtattccc
      601 tgtaccagat ggagatacag gaactaatat gtcattaaca atatcttatg ccttaaaaga
      661 attagcaaaa gttgaaaatg ataatatatc agatatcgga aaagcattat ctaaaggttc
      721 tttaatggga gctagaggaa actcaggagt tatactttca caaataatca gaggtatagc
      781 taaatctata gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag cttttaaaaa
      841 tggttcagac acagcatata aagctgtaat aaaaccaatt gaaggaacta tacttactgt
      901 agttagagaa agtggagagt ttgcaataaa aactgcgaaa aaagagaaag atgtagtaaa
      961 gtttttaagt atgcttgtga aggaatcaaa ttcttcatta gaaagaactc cagaccttct
     1021 aaagaatcta aaagaagctg gtgtggttga ctctggtgga aagggtcttg tacttatata
     1081 tgaaggaatg ttagcttcta taaaaggcga taacatagaa ataaaaaatg ttgacctaga
     1141 tactaatatt tcaacaagta tggattttac aaagagtact actagcacag atgatataaa
     1201 atattgctat tgtacagaat ttattttaga aagttctaag gtagaagata ctaaaataag
     1261 agacataatg atggcttatg gtgatagcct agcagtagtt ggagatgatg gagtcataaa
     1321 ggtacatgta cacactaatg acccaggaaa tgtacttcaa gaagctctaa agtacggtca
     1381 attattaact ataaaaatag aaaatatgaa gttacaacat gaaaatacat tactggatgt
     1441 agaagaaaag aaagaaaatg atagtgaacc tttagaagaa gaaaaagagt ttgggtttat
     1501 agctacttct atgggtgaag gtttggcaaa tatatttaaa gatttcggag ttgaccatat
     1561 aatagaaggt ggtcaaacta tgaatccaag tactgaagat tttatgaatg ctataaagca
     1621 tataaatgct aaaaatatat ttattttccc aaataacagc aatataataa tggctgctaa
     1681 tcaggcaaaa gaattgagtg ataaaaatat aatagttata cctacaaaaa atacgcctca
     1741 aggatttgct gcattagtaa cttttaatgg agaattaagt gaagatgaaa ataaagaagc
     1801 tatgatgaat gctttaaact cagttaaatc aggtcaagta acttttgctg ttagggatac
     1861 agtgatgaac gaaattgatg ttaaagaagg taatataatt ggaattgcag aagggaattt
     1921 attatcagca ggagattatg tggatgaagt aacatcaaac cttattgaaa agttggttga
     1981 tgaagatact gctataataa cattattctt tggtgaagat gttacagaat ctcaagcaaa
     2041 tgaactacgt acttctttag aagaaaagtt tgaagatgta gatgtagagt tatattatgg
     2101 aggacaacct ctttattatt acttaatttc agttgaataa tgattataag aacctgtatg
     2161 accaagtagc ttttataaaa ttggttgtac aggatttttg tgtagtaggt gatatatttg
     2221 ttggatttat ataaagatgt tcaatatgtt aagggaatag gacctaaaaa agcagataaa
     2281 ttaaataaac tgggtatatt tacactaaaa gacctattgt attattttcc aagacaattt
     2341 gaagatagaa ataatctaaa gaaaatagca caattagaag atggtgagaa agttacgata
     2401 aaagcagtaa tatcaagtat aaatacattt agcccaaaag aaggtatgac tttaacaaag
     2461 attgatgtaa aagatgagac aggctcggct aaattagtgt tttttaataa gagttatata
     2521 aaaaatactt ttcgtccagg agattcaata ttagtttttg ggaaggttaa gaaaaaattt
     2581 aataacttag aacttacatc atgtgaatta gaatatctta ctaattcacc taagaataca
     2641 tgtagattta tgcctgtgta ccagcttaca tacggtgtta caaataaaga aattatgagt
     2701 ataattagaa cagtacttga agataaagaa ttaattatac aagaatatat gccacaaaga
     2761 attatagaaa aatataggct atgtagtata gattttgcag ttagaaacat acattcacca
     2821 agtagtaagg aatcattaaa aatagcttta tatagaattg tatttgaaga attacttata
     2881 ctacaactag ggttgtttgt atttaaaagt ggcagaaata aagaagatgg aataaagttt
     2941 gaaacaagta aagacttaaa aaaaataata agtgctttac catttaaact gactaaagct
     3001 caaaacaggg cattagatga aattatacaa gatatgaatt tagagaaaat aatgaataga
     3061 ttggtacaag gtgatgtagg gagtggtaaa acagtagtag ctctcttagc acttgcaaat
     3121 tgtgttttaa atggttatca gggcgctttg atggcaccta cagagatatt agcagggcaa
     3181 cattatattt cacttactga gtctctaaaa gattttggaa taaatgtagg tctattaata
     3241 ggtagtctta caaaaaaaca aaaggataca gtattggaac aaataaagaa taatgagatt
     3301 gatatattaa taggtacaca tgcactaatt gaagataagg ttgaatttaa taatattggt
     3361 ttagttataa cagatgaaca acatagattt ggtgttatgc aaaggagcaa actttcattg
     3421 aaaggagcaa atcctgatat attagtaatg acagcaacac caataccaag aactttagca
     3481 cttatactct atggagattt agatatttct attatagatg aacttcctcc aggaagacag
     3541 ccaatagaaa caattgctat tgaaaaaagt aagcgagata gagcatataa taaccttgta
     3601 agaagagagg ttgaatcagg aagacaagtg tacattgtat gtcccttggt agaagaaagt
     3661 gaagccatag aagccaaatc agcagttgaa cttgttgagg agttaagagc agaatatttt
     3721 cacgacttaa ggcttgggtt gcttcatggt aaaatgaagt caagtgaaaa agatgaagta
     3781 atgagacttt ttaaaaataa agaaatagat attttagttt caacaacagt aatagaagtt
     3841 ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa atgcagaaag atttggactt
     3901 gcacaattac atcagcttag agggcgagtt ggaagaggta gtcataaatc gtattgtgta
     3961 ctaatttatg attctaaaac agatgtatgt agacaaagaa tggctataat ggaagagacc
     4021 aatgatggtt ttaagatttc agagaaggat ttagaaataa gaggacctgg ggaatttttt
     4081 gggacaaggc agcatggtct tccagaactt aaggtagcaa acttgtttaa gcatataaaa
     4141 atactgaagt tagcacagca agaagctcga tatatattgg gtgaagataa taatctacaa
     4201 ttaaaagaaa acatggcact caaaaaagaa attatagata aatttaaaga tacattgaaa
     4261 gaaatttcat taaattaatt ttaaattgtg ttaaaatatt actaatgtaa taatgtgctg
     4321 aagggagatt gtcatattga gagtaatttc aggaaaagcg agaggattaa aactaaatac
     4381 tccaaaaaat gaagatgtaa ggccaactac agatagagta aaagagtctc tatttaatat
     4441 gataaattca tatataatgg aaagtgaagt tctggattta tttgctggga ctggttcttt
     4501 aggaatagag tgcttatcta ggggtgccaa gtcatgtact tttgtagata tcagtaagga
     4561 aagcatagat attgttaagt ccaatataaa aaaggcaaga gttgaaagtg aaagtattat
     4621 tttaaacctt gattttaaga ctgcaataga taaacttaaa ttacaaaata gtaaatttga
     4681 cataattttt atggaccctc cgtattataa aaatatgttt atagaagcta ttgaaaaaat
     4741 tgataattct aatctattga acgaagatgg aataatagtt gtagaacatg atacgaatga
     4801 tttatttcca gataagatat gtaaattgga gaaaactaga gataagaaat atggaaatac
     4861 tacattaact ttttataaga tggaggcata atatggaaaa taaacctaga aaagctatat
     4921 ttgcaggaag ttttgaccca attactaatg gacatttaga tattatttgc agggcttcga
     4981 agttatttga cgaattacaa ataggagtat taaataatcc taataaaaag ggtctattta
     5041 gctttgatga aagagtaaaa cttatagaaa aaagtacaag ccatttaaat aatataaaag
     5101 ttgtgacttt tgatggttta ttaataaatt actgtcagga aaatgggatt ggagcattag
     5161 ttagaggtgt gagaagtggt gctgatgttg attatgaact tcagatggca catatgaata
     5221 gagagcttaa tccagatata gagactataa ttttaccaag ttgtacaaaa tattcattta
     5281 taagttcatc tttaataaaa gaggttttgc tctttgatgc tgatataaaa aatttggttc
     5341 caaagattgt tttggaagaa ttaaagaaaa aaactagtgg gggtaattga tatatgaaga
     5401 tagatttaga aatgattgaa ctatttgagc agttagagga gatatttaga agtgcttcta
     5461 caatcccttt ctcacataaa tgcacagtgg ataaagatga ggttttagca ataattaatg
     5521 atataagaac tttaatacca gaagaagtaa cacaggcagt atggattaat aaagaaagaa
     5581 ataaaataat aagtcaagct aagcaagatg ctacaaatat agttgaacaa gcgaaaaaag
     5641 aagctgagag aatacaacaa gaatatcaag ataatataga ggaattaaag aaaaattctg
     5701 aagatgtagt aaaggcatat gtagaatcca gcgaacctgt tgttcaagca gaccaaagag
     5761 caaaagatat agttgataga gctgagagaa tagccaatga gataagaatt gggtcaattg
     5821 aatatgcaga ggatgtgctt tctagtgttg agtataattt aagagaaata ttagaagaag
     5881 taaaaagaga taaagctgaa cttagaccta aaaaataaat tttaaataaa taaaaatgga
     5941 gctatcacac aaaaaattcc aatttatgtg tgatagctct cttttaatat agcaattttt
     6001 tgttatataa ttgtgctttt tagattactt atatcttatt ttgatattat aatgtagttt
     6061 aatcttttat ttgttgaaat caacttattt atttgtataa ataatatagg ttattttcaa
     6121 atttttttaa taattttacc actgtcttct tttagattag agttagactg tgcaaaatta
     6181 taaaataata ctaaatcatg tgcaattatt tggtatggca ctacatgtag aaaatataga
     6241 taataaaatt aaggaggtag tatgaaggaa agggctaaaa aaatattaga ttttattatt
     6301 aaaaataaca atgtaacatc acaagaacta caagaaaagt ttaatgtttc aaagagaact
     6361 atttattacg atattctagc tataaataaa caactaggaa aaagtggtaa tataaaaaat
     6421 gtaaaacaca aatttatatt tgaaggtaat ttatgtgatg caaggaaaat aatttcaact
     6481 gaagaagata aatttttaga tagtgattac agaaaaactt ttatattaaa caagatatta
     6541 cttggcgaaa aaatatcaat agaaaaacta acaaatgaaa tgttactttc aaaaaacaca
     6601 gtagtacaaa caataactga tgtaaaaaaa taccttcaaa caatgggatt aagattagag
     6661 tataagggaa agtacaaaat tataggtgat gaatacgtta ttagagagct gtttctgata
     6721 attgttcaag aaaacgtact ggaaattaat tctataagtg aggaagtaag tagtttcgat
     6781 acaaaaggac atataaaact tactgattat tctttattaa atctaactaa gtttgtggaa
     6841 tttctaaata aaaggataag agatgggaaa acattatact catataaata tctaaatgaa
     6901 gctaaaaaaa ttagttactt tagtaattgt aaagaacttt tatgtgaaga agctaatgaa
     6961 aatgaacaag cctatatatg cacttacata agctcacttc ccagcttaaa tagtgaagtt
     7021 aaggaagatg ttgttgaaga atatgtagat aaacttatag ataagtttga agtaaataca
     7081 gcaataaaat tagaaagtaa gcatgagttt aagaaaaata ttttaaggca cctccactct
     7141 tcttacaaca gaatacgatt taagtttcct attagaaatc caatgttaga tgagactaag
     7201 tataaacatg agtcacttta taaaattata aaatcaatta tagagaatga agaagagttt
     7261 cctgtatttg aggggataag agaagaagaa ataggattta tagcagcgta ttttggagga
     7321 tatttaagag ggtctaggga taatggtcta agaagaaata aagtcttact agtttgtcct
     7381 aatggtctta tggtatcaaa gagtttggaa atacaactat ataaatacat accaaccata
     7441 gaaatagtag gtatagtatc cattaagcaa ctaaaagaag taaatgtata ctacgattac
     7501 ataattacaa ctatagatat acaaaatgta aacaacgtaa ttgttgtaaa tcctctttta
     7561 acatcatcag atgttcaatt acttatgaac aaactaataa gtgtaaaaga aaacgagaag
     7621 tatttcaatc ttgaattaat aatacaagct ataagaaaaa atggagttat caataatgaa
     7681 gaagcactaa aagctgattt attaaacata atacacaaga tagatgaagg agagatgtat
     7741 caaccaatgt taaaggaatt aattaatgca gaaagggtca atataataaa aaatgttaga
     7801 gattggaaag aagcaataaa aatagcctca aagccattgt tagaagataa ctctatagaa
     7861 gagttatata tagaaaatat gataaagtca gttgagaaat atggaccata tattgtttta
     7921 gctgatagat ttgctctacc acatgcaagt tctaaagaag gagtaaacaa gcttgctatg
     7981 tctttactaa tagtagaaga tgaagttgat ttattaggaa aaccagtaaa tatatttatg
     8041 gtattagctg ctgtagataa cactactcac attagagcat tagcatcact atcagagatg
     8101 atgtacgagg aagaaaatgt aaaactaata attaatggtg ataaaagttc aatactagaa
     8161 cttataaata agcaaaatta ggaggaatga aaaatgaaga tattagcagt atgtggtttt
     8221 ggtgtaggtt catcaatggt attaaaaatg acaatacaaa aggctctaaa gcagttaggt
     8281 atagaatgtg aagtagaaaa tacagatata aattcagcta gaggtgctga ttgtgactgt
     8341 atattcacaa gtcaagaatt gttaaatgaa ttaaaaggaa cttgtaatgt agatgtatat
     8401 ccagttaaaa agtacatgga tttagcagaa gtcacagaag ttgttcaaac ttttttaagt
     8461 gataaaaatg ataagaaata gtataagttt taaaaagtaa atttaagtta atcagaataa
     8521 aataaaaact tactaagttc aaaaatagta agaaagagag gagggattta gtggtagaaa
     8581 acttaaacag ttgacttatc actaaaaaat ataatgcttg attttatttc aaataatatt
     8641 ttacgtaatc catctatgtt aattggatta atagcagcat ttggtcttgt actacagaag
     8701 aagaaagcat ctgatgttat aaaggggtca ctacttgcag ctttcggaat tataattatg
     8761 gagactggaa caggtatgtt agttagttca atagcaccaa ttaatggagc tttccaaagt
     8821 ataagtggtg gtgcagtaac aaaaggttta agtgatgtta cttttactgc aagttatggt
     8881 ggaattatag gtttagcaat gtttgcgggt ttagctattc acctaatgat agcaagattt
     8941 actcctatta agacaatctt cttaacaggt cacatgcttt attggttccc attcatattt
     9001 gtagcttcag gagttgaagc tggattaaat ggtggagtat taatagcttt tgcagcagta
     9061 ttatcagcac tctattggtc aataatgcct tggttattaa gaaaatatgt gtttgatgtt
     9121 ataggagatg attcttttac attaggtcat ccatctggtt gtttagcact tatatcagga
     9181 tttgttgcta aattagtagg aaataaagaa aaatctacag aagatataaa catacctcaa
     9241 agtttatcat tttttagaga aatatctata agtggtggaa tggcagtgtt tttaatgttt
     9301 atagttgttg ggatagtaat accaggtgcg tttaaagata gtaatgagtt tattggaacg
     9361 tatgcaataa agcaaggtct ttcttttgga gcaggtctta taataatgct tcatggggta
     9421 agaatgctaa taaatcaaat aatgccagca tttcaaggta tatctgaaaa agttgtgcca
     9481 aatgcactac ctgcttttga ctgtccaata ttatttaatt ataaaccaaa tgctgtatta
     9541 ataggattct tagttgctat gctaacatct acagctatga ttttaatatg taactacttt
     9601 aatgtattta atttgctact tataccactt gttataactt cattctttga gtgtggagca
     9661 gcagcagtaa taggagaatc acaaggtggt atacgtggag ctataatagg tacagctata
     9721 gctgcaatat ttatggtagt tttagtagga atatctgctg taatttactc aggaactatt
     9781 caaaattgga tactaatatt tggaggtaat gacctttcat tatttggtat aatcagtagg
     9841 tttataggtt cactatttgg aggaatatta tagatatgaa atttaaatac atagatgaga
     9901 taagaagatt aattgatata atagaaaacg aagaaaaagt caatatggaa aaagcagtac
     9961 aaatccttgt tgaagcaata gaaagtaaaa atgctatatt ttcatttgga gcttcacatg
    10021 caggaatatt aacagaagaa ttattctata gagcaggtgg ctgtgttctt ataaatccag
    10081 tatttgcaaa atctctaatg ttagatacag cacctataac tcacacaagt aagatggaaa
    10141 gattagaagg atatggtaca gctctagctg ataaaactcc aattaaaaaa ggagatgtag
    10201 ttatagttca ttcagtatct ggtagaaatc cagtaagtat agagtttgct atgaaagcta
    10261 aggaaaataa ggcaatagta atatgtataa ctaatttaag ttactcaaaa agtgtatctt
    10321 caagacattc ttcaggaaaa aaactttatg aagtatgcga tttagtgata gataatcatg
    10381 gtcaaaaggg agatgcctgt gtagagatag atggattaaa ccaaaaagtt agtccaactt
    10441 ctactgtaat agcaactact gtgatgaact ctattatagc tcaagctact caggaattag
    10501 ttaataaagg gctaaagaat cctccgattt tctatagtgc caatattgat ggtggagatg
    10561 agttaaataa gaagattttt gatgaatata aaagtgtaat tcattatgaa tattaaggtt
    10621 aaaaaaatat gttaatgaac tttgaagttt atcaacaaag aatattaata atatataata
    10681 aatgctatca tttaaaagct gtaggtaaaa atctacagct ttttattatt gtagattaaa
    10741 aagatgcgta attaaatttt aaattggaga aaataaattg gaaggtatca attataaata
    10801 ttagaaacgt tgtcatagaa aaaaactata ttaaaaaaaa gtacatagaa aaaactgatt
    10861 agacaataaa tacatgcttt gataatattt taaatggagt gaaaatgtgt agaattataa
    10921 tgaaaaaaga aaataaaaat gaaaaaagaa aataaaatgg agggaataaa agtgggtatg
    10981 ttaaaaaaat tatgttcact agctatggta gcaattatga ctacaacact tataacaggg
    11041 tgttcaagtg gaaatggaaa agattcaaaa aaggatggcg aaaaagaaaa actagttctt
    11101 tggatgccac cagtagaaaa taatatgaag gaagtttggg accctatttt agataaattt
    11161 gaggaaaaaa ataactgtga agtagattta cagataattc cttgggataa ttattctgaa
    11221 aaatttgcaa cagctataag tgctggcgaa ggtccagatg taggatatat gtatgcagaa
    11281 atgtatccac agtttataga aagtggagca gtagaagatt tgactaacta tgcaactaaa
    11341 gaggataaag agcaatctat ttatataaaa acatctgaaa tgatgggtgg aatgtatggt
    11401 atgccattcc aagcagcaaa tccaggggta ttatactata acaaggatat tttagagaaa
    11461 ttaggagaaa aacctcctaa aacatgggaa gattttaaaa gaatttgtaa aaaagcaacg
    11521 caagatactg atggtgatgg aaagatagac caatggggat tagctcaggg atggggagct
    11581 aaaacttttg gtaacttaaa ctggaactgg tatccttatt tatggcaagc tggtggagat
    11641 atatttaatg atgatttaaa atcggttaaa tttaatgata agagtggttt agaagcagct
    11701 aagttcttaa aagaattaca agcatatgtg cctgaagatt ctttatctaa agatagtaat
    11761 gaaatgatag aaagtgtatt tggaccaggg aaagcagcat ttacaataat gttatcttct
    11821 gctgcaacaa gtgtatttga tgaatcattc ccggatttaa attggggatt tgtaactgga
    11881 ctagaagata agaaagcagc aacttttgga gcagtagacc atttatcttt aatgtcatct
    11941 gcgaaaaata aagaacttgc atacaagtta atacaacata tgttaagtgt tgaatcaatg
    12001 acagaatttc ataaagcaat accaagagca ccaataacaa aaggtgaacc atatcaaggt
    12061 gatgaaagat tcaaagaaat ggtggaaaat gacaaaaatg tatatagacc attagtagta
    12121 ggacctcatg gagttgaaat atatgaatat ttatggaaag aacttcaaac tatgatttca
    12181 ggagataaaa ctccaaaaca agcgttagat gatgctgcaa aatattcaaa tgatttatta
    12241 gcacaataaa agtttaaagc tgtaatatgt tgtaaaggac tttacagatt aaaattagca
    12301 ttgtatttat aaatacaatg ctaatattta tattggagag gataaaatgg aaaacttagt
    12361 tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa ttaaatatta aaccatatat
    12421 gtatatattg ccattgggaa taattttagt ggcgttttat gtgataccaa taataatgtc
    12481 aatatacttt agttttacaa agtataatat tattagtcca gctactttta taggtttaga
    12541 aaattataaa aaattattta cagatgaaac attaaaagta tctattataa atactattaa
    12601 atttactgtt gttgtagtac cttgccaaac tatattatca ttgattttgg ctgtttggat
    12661 tacaggaaaa ggaaatagta aaatagcttc atttgctaaa ggtgctatat ttataccagt
    12721 attatcatct atggttttga taggtatggt atggagggct ttattgaatg gagaaggttc
    12781 tattatatat caggtattag gtgcctttgg aatagaatca tctaaacttt taggagatag
    12841 taagacagca ttacctacac ttatgtttat atctatgtgg aaaaatatag ggtatttcat
    12901 ggtaatttat atttcagcta taatgaattt accaaaacac tgctatgaag tagcgaaggt
    12961 tgatggggct acaaaacttc aagaatttat aaaaataact gtacctttat taaaaccaac
    13021 tacaataatg gttgtatttt tagggtcgat atggtcatta caagtatttg acttaattta
    13081 tactgtgaca ggtggaggtc caggtatatc tactatgagt atagttatgc atgcatttaa
    13141 cttaaacttt aaaaacttta attctggata tgcaatgact gtagcaaacg tattattttt
    13201 gttaatagca gtagtttcga ttttacaaaa taagcttgta agacgagaca attcagattt
    13261 ttagaaaggt aggtataata tgaaaacagc aagaagatta ttaagtgatg gtttactttt
    13321 atttatagcg tgtgcaagtt tggtaccttt tatatacatg ttgataatat cattgaagat
    13381 aacatataac tcatatagtt tggatatatc tttttcaaca gtaacccttc aaaactatat
    13441 agatattttt actaaaaaag gatttgcaca atattttttt aatacagcaa tagtatcttt
    13501 ttcaggtgta ttattaaatt tagtatttag tacattagca ggatattctt ttgcgaagat
    13561 ggattttaaa ggtagtgata agttattttt atttatgata atgactttaa ttataccttc
    13621 ccaagttaca atgataccac tatatataat aatgaaacac ttaggatgga taaatagtta
    13681 tcttgcactt attatgccaa taccaactgc atttggcgta tttataatga gacaagctat
    13741 tttaggagta ccaaaagagt tattagaatc agcaaagata gatggttgct cggattttag
    13801 aattttaata caaatagtgt tgcctttaat taaaccagca ttaattacac tagcaatatt
    13861 tacttttatg ggagcatgga acgagtttat gtggcctctt atagctacaa ctaaagatgc
    13921 aatgagaaca ttaacagttg gattatctac tcttaagaca ttccagataa ctaattatgg
    13981 acagatgatg gcaggggcta caataacatt tttaccgcca tttattttct atttgatttt
    14041 gcagagtaaa tttgttgaag gtgtgtcttt gtctggtata aaaggttaaa tagtctaatt
    14101 taataatata taaagaaact tatataaata tggaggaatt taaactatgg aaatttcata
    14161 tgtactgaaa gattgcacat tcaaacacaa taaatattca gttatagaag ctgaaacttg
    14221 gaaagataag gatttggata ttgtagtagc taaaggtgaa aaatttgcat ttcaaattat
    14281 gttaaaagca acagaagaat ttaattgtac aattgataaa agcagtgcta tatcatggaa
    14341 aggcctagga aatagagtaa ggttagcatt aaatgtgcct aatagtttag aaaataattt
    14401 tagtataaat atattaggat atgttcaaga tgatacaaag gcatttgtga atgatgctat
    14461 tttaagggat aaagatgtgt tagtagagca atatttgcct caaacatttt ggattgaagg
    14521 tcaagtacca gaagattttg aagaaaataa ccttgatata ggtattgata tatttaaaag
    14581 ctttggatat gaagacgaag aaaaagtttg tacaattgat gttcctgtaa aagtaagaaa
    14641 tgttgtgcta aagcctttag atgaaagcaa gttcttttta gatttatggc aacatccaag
    14701 ttgtttagct agaatgtata aagttgagtt atggtctgat atacattttg aaatagtcga
    14761 taactattta aaagaattag catctttagg agaaaaagtt gcaacagtta tagtttcaga
    14821 ttatccatgg gcagggcaaa gttgttataa agtttataaa aatccatcaa acttatatga
    14881 atataacatg gtaagtgtat caaaaggatt ggatggaaag attaaatgta actttgagag
    14941 tatggatagg tatataggca tagctgataa gtataaaatg gctaaggaga tagatttatt
    15001 tggtctatta ggtaattggt gtgctggtga gtttggaaat cctgtagaag gttataagga
    15061 ccctataaga gtaagatatt ttgatgaaaa ggataaagta tttaaattta taaacaatac
    15121 aaatgattta aaagaatata taggattagt attaaatcat ttaatagagt gtggattatg
    15181 ggacagagta aggattatag ctgatgaacc aaataatcca gaagtagtca aagagtgtat
    15241 agaatttata aattctactg taggtactca tcaagttaaa tataaatcag caactcacga
    15301 ccaaaacttt ttagacagag ctaaagatga aattgatgat atgtcaataa atttaaaact
    15361 tactatacaa aattataaag atatagaaag tcttaagaaa aaaatcaatg acaagggtgg
    15421 gatattgact tggtttgtat gctgttttcc agagaaacca aatagttttt taagttctcc
    15481 ttttgtagaa aatagaataa taggatggta tacttattac tttggtcttg atggattttt
    15541 aagatgggac tataatttat ggacagaaga cccttggaaa gattcaagtt acaaattccc
    15601 tatttggaaa gctggagata tgttctttgt ttatcctgga aaagatttaa agccagttag
    15661 aagtgtcaga atggagaatt taagatttgg aatacaggac tttgagttat ttacaatgtt
    15721 agaaaaagaa aagggaagag agtatataga agttgagctt atgcaagaat tattaaataa
    15781 aaaagaaaat gctgaaatta aaggttttgg tgacatagaa ctaggatatt cacttgataa
    15841 ttggggatat gataaagtta aaaaatatgt attagagttg ttagacagag tataaaaaat
    15901 ataagagtgt ctcaaaatga actttttagt tcatgaggca ctctttttgt atgaaatcaa
    15961 tatagtttca ttgtttcaac aatgaaaaat aggattattt ctattctaag acagcccttt
    16021 agttttttag ttgtattcaa tgttttaaaa ctaactataa acgctataaa gtttataaat
    16081 gacaataatt atatataaca tgatgtactt taatttgcta taatagggat tgaataatat
    16141 tatatggaag ataacattgt ttggggggat taatataaaa agaataggta ttattataat
    16201 gtttttttta agtatatctt ttattggatt ttatcttgta ataagagata acagtgatac
    16261 agttaagatg caacgaggga taaatatagg taatgcacta gaatcaccta aggattttcc
    16321 gtgggatgta aaaatgtcaa ataagttttt tgatgatata aaagatgcag gttttgacac
    16381 agtacgtata ccagtaaggt tttctgatta tacgtcagat tctgataatt ttaaaattga
    16441 tgaagaattt tttaaaaaga ttgataaata tgtagattat gcattggata aagacttaat
    16501 agttgtgttg gatttacacc attttgaaga aattatgaaa gaaccaagag ttcataaaga
    16561 aaagttttta aaaatatggc aacagatagc taataggtat caaaaatatg ataaaaaatt
    16621 ggtatttgaa ttattaaatg aacctaaaga aaatttatat tcacagttac taaacgaata
    16681 cattgaagaa gcaataaaga taattagaaa aactaaccct aaaagaacta ttatagtagg
    16741 accatataat ttctatcaaa ttgattattt gaatgaatta aatattccta aagattctaa
    16801 tatagtagtt tcctttcact attacgagcc aaatgatttt gcatttcaag gaaatatcta
    16861 tcataaggga tttgagcact taagcaatat tacttgggaa ggaacaaatg aacaaatgga
    16921 ttatttaaaa aaaaggtttg atactgtgga aaattgggca aataaaaata aggtaaaaat
    16981 cttcttgggt gaatttggag taacaaaaga agcaccagaa acttcaagga gagcttgggt
    17041 aaaagctgta agagaagaag ctgaaaaacg aaacttttca tgggcttatt gggagcttgc
    17101 ttctggattt ggtatataca atcaaattga agggacttgg gatagagaaa tattaagtgc
    17161 attaatagaa aagaggtgat tttatgaaga aaaattcaaa aaagatactt ataactttaa
    17221 ctattattac taatatagta tatatattgt ggagaatttt ttatacagta cctaaagaag
    17281 aagggatgtt tgccctaatc tgtgccataa tacttctttt tgtagagata atgggtatga
    17341 tggaaatgtt tgtgcattat tatggtatgt caaatattga gtaccctgaa aaaccaatca
    17401 taagtgaaga actttatcca catgtagatg tattcattgc aacatataat gaatctgttg
    17461 atttggtgag aaaaactgta aatgggtgta ttcatatgca gtatccagat aagaaaaagg
    17521 tacatatata catatgtgat gatggaaatc gtgaagaaat gtgtattcta gcagaaaaaa
    17581 tgggtgtaaa ttacataact agaactgaaa gagaaggagc aaaagctgga aatttaaata
    17641 atgcaatgca acatacaaat tcccctttga ttgctacatt tgatgcagat atgataccaa
    17701 tgcatgactt tttaattgct acagtaccat actttttgaa aaatgagcaa gctaaaaaag
    17761 atggagaaaa agaggagtat gaaaaggttg gttttgtgca aactccacag agtttttata
    17821 atccagactt gtttcaattt aatctgcact ctgaaggaag gattccaaat gagcaagatt
    17881 atttttacag agacattcaa ttggctagaa ataggacaaa ttctgtaatt tatggtggta
    17941 gtaatacagt tatttcaagg gaagcacttg aagaagttga tggattttat acttattcta
    18001 ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa ggggtataga tgttatgcta
    18061 ttccagaagt acatgcatca ggtttatctc cgacagattt aaaaagtctt attaagcaac
    18121 gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg tctaaatatt ttgtttagga
    18181 gaggtttagg tttttggcag aaaataagtt atatttcttc aattacttat tggtatgcaa
    18241 gtataaaacg ctttgtatat ataatggcac ctattttatt ttctgtgttt aatgtgattg
    18301 tagtaaaatg tacgttactt caagtattgg tattttggtt gccaatgtat attttaagta
    18361 gtttatctct taaaatattt tctcaaaata taagaaatac tagatggacc aatatatatg
    18421 aaactataat gtttcagtct ttaatgccag cagtaatact tgaaacgttt gcaatatcta
    18481 aaaataaatt ttcagtaaca aataagagta aattagaaga aaatagaatg tataagtttt
    18541 tacagggaat accttatttt atttacatgg tcttatctat cataggaatt ttaaaaatgt
    18601 ttgtagcaat atttaaaatg agttcaatga catattcagt tgtattattt tggttgattg
    18661 gaaacctttt taaccttgtt atggctacat tatttatatc agggagacag cagttaagaa
    18721 agtctgagcg ttatattgct gaaattgatt ttaagttaaa gcaaaattct tatgttcttt
    18781 cttcaaaaac aattgatata tcagaaaatg ggtttgcatt tttgctagaa aatccagaat
    18841 atatttcccc agaagaagag tttgaggtag agtttagaga aaaatctgga aatgaaatgt
    18901 atatagctaa tatgaaagca aagattgtta atgttgtgga agtaaattca aagtggaaat
    18961 atgcagctta tattacacat attgaggata gtgaaataga taattggatg tgtattgttc
    19021 acgatagaat acctacttta ccaatgacaa tatctaatca actaggtttc tttgatgatt
    19081 tgcaaattaa tgtaaaaaaa cgtattgaaa aaactagaac attatcacga agaagtccaa
    19141 gaatcaatat gaattttcag atggatataa aaaatacagg taaattaaga atagtgaatt
    19201 ttaattatca atatgtatta ttaaattttg agaataaaag tatttatcct aaagaaatag
    19261 cattggaaat taatgaaaat attgtgttag aatgtgattt gtgtgagggt aaaattgatg
    19321 aaagaggtat cttatataaa gtaaataaca ttgacagcat aatgcaaaat ttctttttaa
    19381 gagatgaaat gatggattgg atattacaaa ataagacaat tcttgtttca aagcctagtg
    19441 aaaagaagga aaaaagtata gatgagtttg aacccatgga atatatttag gagagatgaa
    19501 atgaagactt taaaagtgtt tttttgttct acaaaatatc ttattttggt gtttattttt
    19561 tttataaata ctacttttat aagttcagca acaagtgata aaattttaga tttatccttt
    19621 aaaaagatag agactgactt aagttctaag attacatatg aggatacagg tgtaagaata
    19681 gaaacggata gttctaaaag cgataaggaa cgttatttat atatttacca aaatataaaa
    19741 gaaaattgga gcatgtataa taatttttat attgaaattc aaaataaaaa taaaagttct
    19801 cagagaataa atttaagcat acaaagtaaa aatatgcttg aatttagatt aaaagaaggt
    19861 agtgaagtat ttttagaagg taaaaatata atatattcag ataaaataaa agaaggatgc
    19921 atagaagtac caggtgaatt tgaaggcaaa atttatgtga actttaatag ccttataaat
    19981 gaggaaagta atgttgtgct ggactcaaat atgctttcaa acattgttag ttggggaatt
    20041 acgtttattc catctgatga agaacataat attgttatta taaaaaagat atctttattg
    20101 tctgaagaga aattaagatt tttaaataat attaaaataa ttggcgatga agaagtacaa
    20161 ataccagtat tgggtcaaag tataagtcag tatgaagtat taggtcttaa aagtgattct
    20221 aaaattaagt actctcttat gggaaaacaa gataatgtga gcatatctca aaaaggtaaa
    20281 ttaacattaa gtaataaagc caaacctgga caaattattt tacaagttaa tgttgatgat
    20341 aagtttaaaa ttggaaagaa aataacatta actgagtctt ggagtgttaa taaaaaagat
    20401 aaggatggtg ttccatatac attagtatat cctgagcaat caccaacagt acaagatatg
    20461 aaaaaaataa actttatgaa taacatcata acgtttgttc gaattttatt tgtatctcta
    20521 gtaataatat gctttggaat atatttatat tggaaaaaat gttcaaaaac taagtaaaga
    20581 gaggaggtta gattgtgtta ttttcaagtt taattttttt attttatttt ttacctataa
    20641 ctttggtttt atattatgta tttaggttta accgaacgat acagaacatg atattgcttg
    20701 ctgttagttt atttttttat gcttggggag agccaaaatt tgttgttatc atgatagttt
    20761 ccataataat gaactatata tttggtttac tggtagatag atatagggaa agtaaaataa
    20821 aagtaaaagt ttttttagtt ttgatgtgtg catacaatat aggtgtttta tttatattta
    20881 aatatctagc ttttgcgcta agaaatatta gtgaacttat tagtactgaa ttgactatac
    20941 ctagtattgt tttacccata ggaatttcat tttttacatt tcagggaatg tcttatgtta
    21001 tagatgtata tcgtagacat ggagaagtcc aaaagaaccc tttttatgtg ggattgtata
    21061 tagcattttt cccacaatta atagcaggtc ctatagttag atatgagagt gtggcagaac
    21121 aaattctcaa tagaaaagaa acttggaata aattttctat tggtacttgt aggtttataa
    21181 caggtctagg taaaaaagta ttaatttcaa ataacatggc tatagtagca gactatattt
    21241 acactatgaa tagtcaagga gaaatagctg catcacttgc atggcttggt tcaatagcct
    21301 atactctgca aatattcttt gatttttctg cttattcaga tatggctata ggcttaggtc
    21361 ttatgtttgg ttttaaattt gaagagaact ttaattatcc atatatatca aaatcaatta
    21421 gtgagttttg gagaagatgg cacatttctt tgggaatgtg gtttaagagc tatatatact
    21481 ttccgcttgg aggttctaga gtagcaaata aagatattat gattagaaat atgtttatag
    21541 tgtggttatt cactggaata tggcatggag cagaatggac atttgtaatt tgggggattt
    21601 taaactttgt tttcctaatt atagagcgat ttataatgtt tgaaaagatt gaaaatcaca
    21661 attttataaa acatatttat gctttattag tagttaattt tggatgggta ttgtttaggt
    21721 cacctaattt aaaagaagca tataattatt ttaaagctat gtttggaggc aatggagcta
    21781 tatggagtga ttatacatat atgtttttaa aagaatactg ggttttcttt atatttgcat
    21841 ttatttttag tgtacctatt gcaaagaaaa tcaataagtt tgttgttgaa caagctaaat
    21901 atagtatgat atttaacgta ttttatccaa tctcagctat tttattgttt tttatttctg
    21961 tgacatattt agtaactgga agctataatc catttattta ttttaatttt tagttgggag
    22021 gaaaagtatg aatagatttt ttaaagtaag attaataact gcaattatgt tcttaattat
    22081 tttattcgct ttctctatct ttaatgtttg ttattctgga aaagatacga tacaggaaat
    22141 aaaaaattca atagctgaaa aaagtagttt aaaagataca atatcatcta ttgataatac
    22201 agtgaatgac aaagtaatat acaaaaatac gttcattgaa acttatggct atttacaaaa
    22261 attaatgggt aaaaatgagt tttcaaaatt tagtgttgta aaagatactt ctggaaagat
    22321 gcattataca tattttgcta aagctccaaa tccagttggt gtgatagcag atagggtaag
    22381 aaaattttct gatgaaatgg aaaagcaaaa cactaaattg gtttacctaa tgactccaga
    22441 taagtatata aaaggagtga caaaatttcc caaaggaata ccttattctt atagtaatga
    22501 gacagcagat aattttttaa atcaactaaa aaagtataat gtggactata ttgattttag
    22561 agaaaatatt ttaaagagtg gtattccaaa agatgattta ttttttaaaa cagaccatca
    22621 ctggaaagta gaaacttctt tttgggcatt tggagaatta gttgagcaac ttaataaaaa
    22681 atacaatatg aatttagatg aaaatcatta ttatagagat aaggacaatt ataattcaat
    22741 tgtatatcct aagtcatttc ttggttctat gggaagaaag actggtatct tatatggtgg
    22801 tatagatgat tttacattaa tttatcctaa atttaagact aattatactt attatacaga
    22861 ttctaaatct caaaaatttg aattaaaagg aaggtttgaa gagagtttaa tattatcata
    22921 tccttttaat gcagacctta atttaatgga tggtcaatca gataagtatt ttacatattt
    22981 attggggaac agaccattag ttaaaataaa aaatatagaa aatccagatg gattaaaagt
    23041 gctgtttgtt aaagattctc taattgtacc aacagcagca tttttttcct ctgtttgttc
    23101 atctatagat atgatagacc ctagatatta tgatggagat atactagaat atgcaaaatc
    23161 ccatgactat gactttgttt ttatatctgt atatccccaa aatcttacaa aagaattttt
    23221 tccgttttgt gagtaaattt ctaaaaaagt tttctgataa aataaatggc taactggtaa
    23281 aaagttagcc atttattttg agtgttattc taagaaatat tttttttact aaatacttct
    23341 ttatctattt ttccaagtga ttttcctaca gtaattgatg ataccatagc accattaaca
    23401 tttatcatag tacgtcccat atctagtata gggtctattg caagtatacc accaactaat
    23461 ggaaaataag aaccaagacc aactccagag ataacaactg aaacggccat tgtagcagtt
    23521 cctggaagac cagctattcc aaacgaacta attgttataa caattaacaa cataacataa
    23581 aagctcatat ccatgtttac tccagccata ttggcaagtg ttacagccat taaagcagga
    23641 tatatacctg cacaaccatt catacccata tttgcaccta aacttgctgt aaagcttgat
    23701 acaccctcat caactccgtg attttgtact agactttcta tcgttacagg caatgttcct
    23761 aaactagaac gtgaagtaaa tgccagaagt aaagcttcag ttgaattttt tatataagta
    23821 atgggattta gaccatttaa agttataaaa attaaatgta tgataaataa tattgcaact
    23881 gatatataaa gagctaatat aaactgtaaa acagatacta ttgaactaat accttgtgaa
    23941 gttattgtat ttgcaagcag agcaaccaca gcataaggca taaatttaat aacagtcata
    24001 gacacactta caattatttt gtaagatgct tctataaaat ctataaatgg ttttattgtc
    24061 tcaaaatgtt ttttacttaa tctctttatt gaggtagcta taaatgtagc aaatattatt
    24121 gtagctacaa tatttccatt tgccatagcc attacaggat tagatggtag taatcctctt
    24181 aaagtatcca caagagagtt tatttcttta agttcttgat tttcaactaa agtaggcata
    24241 ttagctccta cacctaactt cattatattt gcaacgataa ttccaacaat tgcagctata
    24301 gcagtagtag caagaagcat acctaaagat ttaaatgtca gagttccaag attgttatct
    24361 ttcatattta taattacttt tataatggat aagaaaacta aaggtactac taacattttt
    24421 aataagtcca taaatccaga accaaatagg ctataccact tacttacttc ttgtagccaa
    24481 atgacttgag ttgtatcatt agggaaacca gcaaaagctt gtattatgat tcccaatatg
    24541 agtcctagta ctgtagcata tattgttctc aatgaaaatt ttattttttt cttttcaaat
    24601 tgtctaacaa taaagaacgt acctagaagt aatattatga aaaaaattgt tttaaaatct
    24661 gtaatcataa gaaattgtga taaaaatgaa ctttccaata tttttctcct tttatttaaa
    24721 aataaatttt tctaagttta aatgatatca atatattaaa aaaatgtcaa tattatatca
    24781 caatttactt aaatagagta aaacaagttg attttgaaaa atgtgattaa gtacagaaaa
    24841 atagggtata ataaatgttt aaagaatttg aattgtggta agaaaataaa aaatagcttt
    24901 tataagattt gatttcaaat taaaatgtgt taaataaata actaaaaatt attataaaaa
    24961 atgttttcat agaatatata tcaaatgaaa agcttaaaat aagtgaagaa attaactaga
    25021 catcaaagga gagataaaat gagagtaata ataattggtg gtgttgcagc tggtatgagt
    25081 gctgcagcaa aattaaaaag aataaaacct gagtatgaag ttgttgttta tgaaaaaact
    25141 gaaatagtat cttttggtgc ttgtggatta ccatactttg ttggtggatt ttttaatgat
    25201 gctgatgaat tattagcaag aacaccagag aaacttagag aagctggaat tgatttaaat
    25261 atatttagag aagtcgttga ggttgatagt gaatctaaga aaataaaagt aaaaaacata
    25321 aaaacagatg aaatatatga agattactat gataaattga tgatagcaac tggagcaaga
    25381 agtattatgc cttctataaa aaacataaaa ttgaagaatg tttctacatt aaaaagttta
    25441 tatgatggag aatatctaaa aaaactttta agtaatgaag ataataaaag agtgacaata
    25501 ataggagctg gttttatagg gttagaagca gttgaggcat gtaagaaatt aggtaaagat
    25561 gtagatgtaa ttcagttaga agatagaata ttgccacaag tatttgataa ggagatgacg
    25621 gatgtattag aagaagaaat aaatagacat aatgttaatt tgcacttaga tgaaatggtt
    25681 gtagaacttt gtggagaaga taaagtagaa aaagttataa caaacaaagg ggaaatagat
    25741 acagatgtag taataattgc tactggcgtg agacctaata ccgagttttt gtcaaatact
    25801 aatataaaaa tgctaaaaaa tggagctata gtagttgatg agtatggaag aacttctgta
    25861 gaagatatat actctgctgg agattgtgca actataaaga atatagtaag taatgaaaat
    25921 gtatatgttc ctttagctac tggagctaat aaattgggta ggatagtagg agaaaaccta
    25981 gcaggaagag aagtatctta tcaaggttca ctatcttcaa gctgtataaa aattatggat
    26041 atggaggcag catcaactgg aattacagaa aggcaagcta aagatttagg cataaatgtc
    26101 aaatctaaat ttatttctga ttataatcaa actcattatt atccaggaag aaataaaata
    26161 tatgtaaagc ttatttatga tgcagataca aaagttattt taggcggaca agtggctggg
    26221 tttaaggatg ctgtccaaag agcaaatgta ttggcagctg ctatctttgg aaaaatgacc
    26281 acaagtcaac ttggaatgtt agatttatgt tatgctccac catttgcaag aacatgggat
    26341 gttttaaatg tagctggaaa tatatctaaa taatatttag attatagcta atatagttga
    26401 caagcaatta agggaatgaa tttacctaaa ctatttttat catggtaaaa ttcattccta
    26461 aatttttatt ccaaattttt attccaaatt tttagaaata aggattccat taaattattc
    26521 atcaaaattt aaacgcaagc ttaatctatc ttctccaaga atagtatttt caaaaacatt
    26581 agtagcattt tctaaataca tactaggtat gtctaaagaa ggactaaaat gagtcagtaa
    26641 aagttgtttg acatttccaa gttttgcaag attagcagct tctctaaaag tcatatgttt
    26701 gtttctaaca gcttttgata tatctaaatc atcaccatac atggcttcac atataaataa
    26761 atcactatct tttatgaaat ctggtattga ttctataggt cttgtatcgg ttgtaagact
    26821 tatcttaact ccctttctta attctcctaa gaccatatct ggagtgtatt gttttgaatc
    26881 taaaactata ttttgacctt cttggagttt gttccataat attttaggaa cttcgttttt
    26941 agttgcctta tctatatcaa atttagggtt tcttctaaaa ttaaaactgt atcctaaaca
    27001 ctctatagag tgttctaaac tgattgttga aatttctaaa tttcttaaaa ctttattatc
    27061 taaagaaaaa ttgccttgag ggttttctat aatttttaaa gtgtatggta catattctac
    27121 taaatttctc atagaacgta tacaatcgac tattccaact ggaccaacta tagttaaatc
    27181 acttgtacga ccactattcc caattgttga taaaagtcct aaaagtccaa atatatggtc
    27241 accatgtaaa tgggttatac aaatcaagtc tatatcttta aagccacatt tttttagctt
    27301 catagatacc tgagtacctt caccacagtc aattagtatt tttctacctt tatagtttat
    27361 aaatacagag gatagaaatc tgtttggcat aggtacattt ccaccacatc caagtaaagt
    27421 taaatctatc attttttccc ctttctaaaa ttaacacaaa tctgttgttt gtttttatta
    27481 tgatatccta ttataattat tttaacatta aatataacaa taaatgtgta tttgagtcaa
    27541 gaaatattgt caaataaagt ttttatataa caatgtataa aaatttggag gacaaaaagt
    27601 gaaaattata tttagaagta tttaaaggag gaaaatttaa tgaaagtatt aaagaaaatt
    27661 actattttgt tattgattac attaacttta gttggttgta gtaacggaga agagaaaaaa
    27721 gtggttgaag gttctaagaa aactcaaaat tctaaaaaag atgaacagcc tataatagtg
    27781 cataaagatg caaatatatt gaaagatagg tatgagccac caaaaggatt taaaagagtt
    27841 gatgtagaaa aaggtagttt tgaatcattt cttaggaata ctaagctaaa aaattatgga
    27901 gagaaagtaa agtattatga tggaagaact aaggaatcaa aaggtattta tgatagtgta
    27961 tttgatgttg atattggaaa tagagattta catcaatgtg cagatgcaat tatgcttatg
    28021 agagcagaat acttgtatca gaataaacta tatgatgaaa taagttttga ttttgttgat
    28081 ggttttaagg cgaagtattc aaaatgggca caaggatata gaatttcagt aaaagatagt
    28141 ggttcaagct ggtataaagc aactgaatat tcaacatctt atgagagttt cagaaagttt
    28201 atggatatag tgtttgcata ttcaggaacg ttatctcttg aaaaagaatt ggagcctgta
    28261 aaagttgagg atatgaaaat aggagatgtg tttatagttg gtggaagtcc aggacatgca
    28321 agtataatta tggatatggc agaaaatgaa aagactggtg aaaaggtatt tatgatagcc
    28381 caaagctata tgcctgcaca acaaacgcaa cttcttatta atcgtaaaaa tccaaatttg
    28441 agtccttggt atagtttaga ttttgaaggt gatttgacaa tacctcaatg gacttttaaa
    28501 agagaccagt tgaaaagatt ttaaaataat aattttatat aataaaagaa aaatatagat
    28561 gtgaaaaaat gtaattagat taaaactaat tggatatgga caaaat
//
LOCUS       DAMJYC010000049        27123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJYC010000049 DAMJYC010000000
VERSION     DAMJYC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.050"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(59..382)
                     /locus_tag="PC472_002231"
     CDS             complement(59..382)
                     /locus_tag="PC472_002231"
                     /inference="COORDINATES: protein motif:HMM:NF020435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDF2727553.1"
                     /translation="MLLVESKNDRSILAKESLEKLGAKNVKIYNMDTGSNVIYSVENG
                     KEHLTISNEKRFPNWNEIKYVRYKLMKPNKTIVQILPPKEEYINLHENCFHLWEIEDN
                     AVPTK"
     gene            complement(400..573)
                     /locus_tag="PC472_002232"
     CDS             complement(400..573)
                     /locus_tag="PC472_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727554.1"
                     /translation="MTNKEMCKSKNLDEREVYKEFGKEICASCINNRRDCESKDCDIA
                     YENWLEKETKNYL"
     gene            complement(578..817)
                     /locus_tag="PC472_002233"
     CDS             complement(578..817)
                     /locus_tag="PC472_002233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727555.1"
                     /translation="MKKYKIKFEEKVTLEHEVIVEIPNEININDICNCIENKCQRVYD
                     IADYIREFNGRQIDFTEDTCGETEMVVESFRKCKE"
     gene            complement(913..1188)
                     /locus_tag="PC472_002234"
     CDS             complement(913..1188)
                     /locus_tag="PC472_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727556.1"
                     /translation="MEYKEYEDLKNRVESYEDLQGSAEFAERVIENLEDVDCPIRIGF
                     KFPNKEDYQKIDLDIAAKDSNSTFIRTELAKAFKEILSKYEMDMENM"
     gene            complement(1240..1398)
                     /locus_tag="PC472_002235"
     CDS             complement(1240..1398)
                     /locus_tag="PC472_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727557.1"
                     /translation="MDDRLEICKKMFPIVTKNTRLLCHYCDGRNICSYDEEKAHELLR
                     NEEGTKNN"
     gene            complement(1501..1950)
                     /locus_tag="PC472_002236"
     CDS             complement(1501..1950)
                     /locus_tag="PC472_002236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727558.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKENCEWQKVS
                     NVGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLS"
     gene            complement(1937..2086)
                     /locus_tag="PC472_002237"
     CDS             complement(1937..2086)
                     /locus_tag="PC472_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727559.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNESN"
     gene            complement(2055..2435)
                     /locus_tag="PC472_002238"
     CDS             complement(2055..2435)
                     /locus_tag="PC472_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727560.1"
                     /translation="MAKIWLDAGTFLEKTIDIEDMFELNLRKVRKANESKKIKLKLNE
                     SKFKKIRKKPTNDTKSKPIKVFNIETGEVRIFKSAKAASKYLKISGDYASCLARENRV
                     TREGWKAEYIQEVSDGISKCGASN"
     gene            complement(2453..2674)
                     /locus_tag="PC472_002239"
     CDS             complement(2453..2674)
                     /locus_tag="PC472_002239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727561.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQHKKYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFWFNFGVVSEWCVEVK"
     gene            complement(2679..2840)
                     /locus_tag="PC472_002240"
     CDS             complement(2679..2840)
                     /locus_tag="PC472_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727562.1"
                     /translation="MSRTAICSVCGEFLVESYDDQFTNTKVMDLHGREFVIVICKSCL
                     EELLRKCED"
     gene            complement(2910..3317)
                     /locus_tag="PC472_002241"
     CDS             complement(2910..3317)
                     /locus_tag="PC472_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2727563.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            complement(3333..3506)
                     /locus_tag="PC472_002242"
     CDS             complement(3333..3506)
                     /locus_tag="PC472_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2727564.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            complement(3567..4464)
                     /locus_tag="PC472_002243"
                     /pseudo
     CDS             complement(3567..4464)
                     /locus_tag="PC472_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
     gene            complement(4479..5063)
                     /locus_tag="PC472_002244"
     CDS             complement(4479..5063)
                     /locus_tag="PC472_002244"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2727565.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVDK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYENKQ"
     gene            complement(5073..5582)
                     /locus_tag="PC472_002245"
     CDS             complement(5073..5582)
                     /locus_tag="PC472_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2727566.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            complement(5660..5977)
                     /locus_tag="PC472_002246"
     CDS             complement(5660..5977)
                     /locus_tag="PC472_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727567.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEEIERDRPID
                     IQI"
     gene            complement(5980..6126)
                     /locus_tag="PC472_002247"
     CDS             complement(5980..6126)
                     /locus_tag="PC472_002247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727568.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            complement(6127..6570)
                     /locus_tag="PC472_002248"
     CDS             complement(6127..6570)
                     /locus_tag="PC472_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2727569.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            complement(6600..6797)
                     /locus_tag="PC472_002249"
     CDS             complement(6600..6797)
                     /locus_tag="PC472_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727570.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            6850..7050
                     /locus_tag="PC472_002250"
     CDS             6850..7050
                     /locus_tag="PC472_002250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727571.1"
                     /translation="MKVGDIMQFSKDILHTLTLEILKEKYDFKSSSEEELLKHYHEIF
                     LKLSEVNNSFSKGDGLSVFKQM"
     gene            complement(7037..7234)
                     /locus_tag="PC472_002251"
     CDS             complement(7037..7234)
                     /locus_tag="PC472_002251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727572.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNTLNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(7294..8085)
                     /locus_tag="PC472_002252"
     CDS             complement(7294..8085)
                     /locus_tag="PC472_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2727573.1"
                     /translation="MNNLQIFEKLEFGQIRMVEVDKKPYFVATDIAKCLGYANTSKAI
                     NDHCRWVTKSYIPHPQNENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPT
                     IRKTGGYIHTTEDMTDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAV
                     ASSDDSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLKI
                     METKKRAIYNPDGSTKITKTPKVTGKGQIYFINKLKLVNQLSMLS"
     gene            complement(8088..8258)
                     /locus_tag="PC472_002253"
     CDS             complement(8088..8258)
                     /locus_tag="PC472_002253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727574.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            8379..8690
                     /locus_tag="PC472_002254"
     CDS             8379..8690
                     /locus_tag="PC472_002254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727575.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSVNSLINNAVHSFCISHWLIY
                     L"
     gene            complement(8676..8813)
                     /locus_tag="PC472_002255"
     CDS             complement(8676..8813)
                     /locus_tag="PC472_002255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDF2727576.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
     gene            complement(8868..9656)
                     /locus_tag="PC472_002256"
     CDS             complement(8868..9656)
                     /locus_tag="PC472_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2727577.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            complement(9625..9888)
                     /locus_tag="PC472_002257"
     CDS             complement(9625..9888)
                     /locus_tag="PC472_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2727578.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            10067..10462
                     /locus_tag="PC472_002258"
     CDS             10067..10462
                     /locus_tag="PC472_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727579.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            complement(10499..10636)
                     /locus_tag="PC472_002259"
     CDS             complement(10499..10636)
                     /locus_tag="PC472_002259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727580.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            10920..11141
                     /locus_tag="PC472_002260"
     CDS             10920..11141
                     /locus_tag="PC472_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727581.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTISILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            11200..11406
                     /locus_tag="PC472_002261"
     CDS             11200..11406
                     /locus_tag="PC472_002261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727582.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            11543..12709
                     /locus_tag="PC472_002262"
     CDS             11543..12709
                     /locus_tag="PC472_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2727583.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETVDILEGILQ"
     gene            complement(12619..13003)
                     /locus_tag="PC472_002263"
                     /pseudo
     CDS             complement(12619..13003)
                     /locus_tag="PC472_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(13243..14637)
                     /locus_tag="PC472_002264"
     CDS             complement(13243..14637)
                     /locus_tag="PC472_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2727584.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(14657..14866)
                     /locus_tag="PC472_002265"
     CDS             complement(14657..14866)
                     /locus_tag="PC472_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2727585.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(14895..16340)
                     /locus_tag="PC472_002266"
     CDS             complement(14895..16340)
                     /locus_tag="PC472_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2727586.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            16450..17244
                     /locus_tag="PC472_002267"
     CDS             16450..17244
                     /locus_tag="PC472_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2727587.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            17295..18617
                     /locus_tag="PC472_002268"
     CDS             17295..18617
                     /locus_tag="PC472_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2727588.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(18717..19934)
                     /gene="cdeC"
                     /locus_tag="PC472_002269"
     CDS             complement(18717..19934)
                     /gene="cdeC"
                     /locus_tag="PC472_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2727589.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(20117..20297)
                     /gene="ssrS"
                     /locus_tag="PC472_002270"
     ncRNA           complement(20117..20297)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC472_002270"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(20412..21317)
                     /locus_tag="PC472_002271"
     CDS             complement(20412..21317)
                     /locus_tag="PC472_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727590.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            21529..21927
                     /locus_tag="PC472_002272"
     CDS             21529..21927
                     /locus_tag="PC472_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727591.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(22050..23066)
                     /gene="ccpA"
                     /locus_tag="PC472_002273"
     CDS             complement(22050..23066)
                     /gene="ccpA"
                     /locus_tag="PC472_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2727592.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(23319..23519)
                     /locus_tag="PC472_002274"
     CDS             complement(23319..23519)
                     /locus_tag="PC472_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727593.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(23553..23732)
                     /locus_tag="PC472_002275"
     CDS             complement(23553..23732)
                     /locus_tag="PC472_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727594.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(23914..24120)
                     /locus_tag="PC472_002276"
     CDS             complement(23914..24120)
                     /locus_tag="PC472_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727595.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(24168..24593)
                     /locus_tag="PC472_002277"
     CDS             complement(24168..24593)
                     /locus_tag="PC472_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727596.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            24765..24992
                     /gene="acpP"
                     /locus_tag="PC472_002278"
     CDS             24765..24992
                     /gene="acpP"
                     /locus_tag="PC472_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2727597.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(25136..26140)
                     /locus_tag="PC472_002279"
     CDS             complement(25136..26140)
                     /locus_tag="PC472_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2727598.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(26154..27056)
                     /locus_tag="PC472_002280"
     CDS             complement(26154..27056)
                     /locus_tag="PC472_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2727599.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
BASE COUNT         8597 a         4637 c         2939 g        10950 t
ORIGIN      
        1 gtaagtctat aaccttctag tttagttcac aaacttacct tgactttatt tttataaatt
       61 acttagttgg aactgcatta tcttctatct cccaaaggtg aaaacaattt tcatgtaaat
      121 ttatatattc ttcttttggt ggtagtattt gtactatggt cttatttggt ttcatgagtt
      181 tgtatctaac atacttaatt tcattccaat taggaaatct tttttcattt gatatagtta
      241 aatgttcttt gccattttct actgaatata taacattact cccagtatcc atgttgtata
      301 tttttacatt tttagcccct aatttttcaa gtgattcttt tgcaagaata gacctatcat
      361 ttttactctc aactaataac attttattcc ctccaaattt tataaataat tttttgtctc
      421 cttctctagc caattttcat atgctatatc acaatcttta ctttcacaat ctctcctatt
      481 atttatacaa ctagcacaaa tctctttccc aaattcctta tacacttctc tttcatcaag
      541 attctttgac ttgcacattt ctttattagt catatgctca ctccttgcat tttctaaatg
      601 attcaaccac catttcagtc tctccacaag tatcttctgt aaaatctatt tgtctcccat
      661 taaattctct tatataatct gctatatcat atactctttg acatttattc tctatacagt
      721 tacaaatatc atttatattt atttcgtttg gaatttcaac tataacttca tgttctaaag
      781 tcactttttc ctcaaactta attttatatt ttttcatttt aaatactcct ttatttcatt
      841 tttgagagtc acaaaacact tcaacaataa tttatactaa aagacatttt gcaactctct
      901 aaactgtttt aattacatat tttccatatc catttcatat ttagataaaa tctctttaaa
      961 tgcttttgct aactctgttc ttataaaagt tgaatttgaa tctttagcag ctatatcaag
     1021 gtctatcttt tgataatcct ctttgttagg aaacttaaat cctattctta taggacaatc
     1081 tacatcctca agattttcta taactctctc tgcaaactct gcactacctt gtaaatcctc
     1141 ataactttct actctatttt ttaaatcttc atattcttta tattccataa tctattcctc
     1201 ctctaaactg ttttaattag atattttatc atttcaaagt tagttatttt tagtaccttc
     1261 ttcatttctc aataattcat gagctttttc ttcatcataa ctgcaaatat ttctaccatc
     1321 acaataatga caaagtaatc tagtattttt agtaacaata ggaaacattt ttttgcaaat
     1381 ctccaatcta tcatccattc ttatacacct cttttgttat cacaattttc acactctttt
     1441 agattcaatc tatactcata aaccctacca gctataaaac ttcctactat aagtattaaa
     1501 ctagctaaga tgttcatttt ctaacatctc cttacgttct aagaactctt ttttaatttc
     1561 ttccaaattc tcgcattcat taccaactac tacatatttt ttgctattca ttatagttgc
     1621 tttacttgta atttcctccc atttaccttc aaattgcttt aaataatgcc attcaacata
     1681 taattcaaga gcataatttt cttgtcttac aattccatat tcatatttat ttttagtatt
     1741 atagggttct ttcaatatat ctccctcata aatttcttca ttatttccag accaacattt
     1801 cgagaatact ccaacattag atactttttg ccattcacaa ttctctttta gcataaataa
     1861 acaatctact gcatcactcc actttattgt ttcagaataa atccacttct cattctcaaa
     1921 atcataacct ctgtacttaa ttagactcat ttctaatcat cccctcgcac tcatatttgc
     1981 ttaatatttt tatagctata tcaatagctt tattaacaga acactttttc ttatttaata
     2041 tcttttcagc taacttaatt acttgctcca catttgctaa taccatctga cacctcttga
     2101 atatattctg ccttccaacc ttctctagtt actctatttt ctctagctaa acaactagca
     2161 taatctccac taatctttaa gtatttactt gcagcctttg cacttttaaa tattctaact
     2221 tctccagttt caatattaaa aacttttata ggtttacttt ttgtatcatt agttggtttt
     2281 ttccttattt ttttgaattt cgattcatta agtttcagct ttattttctt actttcattt
     2341 gcttttctta cttttcttaa attaagctca aacatatctt ctatatcaat agttttttct
     2401 aaaaacgttc ctgcatctaa ccaaatttta gccatatcta attcctccta ccttatttaa
     2461 cttcaacaca ccattccgaa actacaccaa aattaaacca aaattcattt ggatagcaaa
     2521 catagccatc atcaaaagtt ttttcaacag taaattcaac accttcaata tcatcaatcc
     2581 aaagttcttt atatttttta tgttgttcta caccattaat ttccataaat ttctctttat
     2641 caaacttata agttttacct atctcaaatc tcatattgtt agtcctcaca ttttcttaat
     2701 aattcttcta agcaactctt acatataaca attacaaatt ctctaccatg taaatccata
     2761 acctttgtat ttgtaaactg gtcatcataa ctctcaacta gaaactcccc acaaacacta
     2821 catatagctg ttctactcat ttttatccct ccatttttaa cttttaggaa gtaatattgt
     2881 ataattactc cctagactat ttaacttaat taaaaaggta tatcgtcatc atctattgct
     2941 tgaaaacctt gtgggtctaa tcctggtggt acatattctt gtttagcatt attatcattt
     3001 ttactagaaa gtagttctaa agcatttaca ttaaccttag taatagattt ccagcaacca
     3061 ttttcatctt tgtaattata tatatttaac tctccaacag catatatagg cttaccttta
     3121 acaagatatt gcactaaatt ctctacatgt tttcctaatt gctcgcattg aataaaatca
     3181 gttattttat ttccattttt atctttaaac cttctatcta ctgccattga aaaggttatt
     3241 tttggagtac ctgaatttgg aaggtacttc aattctgcat ctgcaactaa tcttccaact
     3301 aaagttattg tattcattta actagccccc ttctattttt cttcctgttc ttctgtatac
     3361 tcaacaaagt aagtataagt tgtcttgcta ttttgcttct ctctagcaac ctttactgta
     3421 tatccagctt tcccaagtaa tcttaataac tccaatctat cttgttcatt taaagaacca
     3481 cttctttgtg catatattct cgccatttta tacctcccct tttctaggaa gcaatatatt
     3541 gatatttact tcctagaagt ttaattttat ttaaatttaa ccttttggct tttcttaatt
     3601 atgtcatcta gttcgtcagg tgaatattga gtaaatgttt cattgaagtt atgaaactta
     3661 tttttactca cattaggagt attcacagct ttactattag actgcttctt atcctgttta
     3721 ttctttttct tcctctcaaa ctcattttga tactctgtaa gttctaaatt agtttttaca
     3781 cctgcttcta tccaattatt taatattgtc tttacatact tataattctt aactccattt
     3841 gctattgctt catcaatagc tcttataatt acatcagctt ccattccatc atctaagtaa
     3901 ctcattaact ctataaagtt attaggagta atcacaccta tatatttttc aaagtatttt
     3961 tttatgtagg tggttttgtc tttattggat tgttcattaa taacaatagt agtaatatca
     4021 ttatttactt taaagtcatt acttactact tccgtgtttt ccggtttccg agaaacccgg
     4081 tttccgagaa acccggtttc cgggaaatcc ggtttccggg aaatcagttt ttcgggattt
     4141 tagcttctga ggattttcaa gtggtatctc atatacttga taatcatatc ctccaagcat
     4201 cttattagta ttagaatctc gacaaggtgt tcttgttata tatccatttt ctatgagctc
     4261 ccttaaaata tttgctgtag catccctccc atttttactt cttttatata aatcattaac
     4321 atagattttc cagtggtcgg gcttactaat cagatatgaa tgtaaacctt ttgcttgcca
     4381 gcttaatttc acatcttcca aacaagtttt atttaaaact acatatggat tatctttgtc
     4441 tttgcttact cttataatcc ccacaatatc acctgccctt attgcttatt ttcataagca
     4501 ttacaaatag tgtcatactc ttgttttgtt aaatctttta tctcttttcc aaatctttta
     4561 aatacttttt ctttcaaact ttctttatca acatttgcat tacttgctat tgcatataac
     4621 ctacttaatt gtttatctgt taaaattcta ttgttagaat tacttttagc ttcatttttg
     4681 ccactagttg cgtcaaaagt gtcactttca gttatgttga gtaactgaat gtacaaatat
     4741 ctagtttgat aggtttctat acctcctagt gcttgtaatt cattagaacc tttaagttgt
     4801 aaatctctca tgggagaagt aaatacaatc tgttctgatg ggttttcccc attaattaat
     4861 gttagagttg catattcatt tgtaaaggtc actatagggc atagcttagc ttcttcaagt
     4921 aatccagttg cttgtggtag aaagtctgct aactcaaaat acttgaagtt agcgaactta
     4981 ttttctccac ttttctttag attcaactta ctaaatttaa ctcttacatc catcaattta
     5041 atgtaaatat tattaatttc catggtcctc acctactctt ttttagcttt tggaattgtt
     5101 agtgtagttc catattcaat cctgcaacct tcaacctcat gacctttttt aataaaatct
     5161 ttaatagtgt tcttatctac ttttacaact tgctctactg ttttatatat agcaggtatc
     5221 ttttcttcat cttctatgac taagctacct gctgactttc ttatacttat atttcctaaa
     5281 actgtttcta cttttttagt cccaagtaat tccatacagt cttttatatt gctttttaat
     5341 ctatcaagag tattcttctt aaccttcttt aactcttgta acctcttaat ctctgaatct
     5401 atagagttta tatcactgtc aatgtttaat attactgaca ctattctagt gtttttattt
     5461 tgtatctctt gttttattat ttcttttatt tcctctagtt tttcagcttc atttcctgtt
     5521 gtttctgtta aaccttcttc tatttctaat aaatctgtag ttaattcata taaagtactc
     5581 ataatttccc tccgtttgtg ctataattag cttatatttt atattatttt ggaatcgagc
     5641 cactcctaat ggctcttttc tatatctgaa tatctatagg tctatctctt tcaatttctt
     5701 ctgaaattaa ttcaaatatc ttgtaatcct cgctttcttc atattctttt atttcaattt
     5761 gtgtatctat aatttttagt aatgactcag caaatatttt taatctttca tttacacttt
     5821 tttctcttaa agcattactt aactctattt catctaatac atctcttttt tctttttttc
     5881 taagttttgt ataaagttcc tcatttttat ttatttctaa attagctcta tttagttgtt
     5941 gctctactgc atttcttact atagttaaac ttttcatgat taatccccct taatttttaa
     6001 tttacttggt aaatacaagt taactaactc tatatctctg ttgtattttc taagaccttc
     6061 aaaacttgct tttatttgct tatcattgca aaactgtaca taagctatta gtactcttac
     6121 attcaactaa atcaccccct ctctaatttc tttcatttct ctaagcattt ctttgatgtt
     6181 ttttccttga tttctagtta taaaatcatc taattcataa ctagaaactt tagttgcccc
     6241 tatatcaact gacttcaaaa gcccattttt tattaactca tatccaaata ctttatctat
     6301 tttcaatctt ttacttgctt cttcaacaga cataagataa tcgggataac ctttacttat
     6361 aacaattgtt agttcttttg gttctaataa ttctattttc gaagtttcat ttaagtactt
     6421 tgagatttta tttttatagt tgtttaaatt catttctaca actttacgga taccttctga
     6481 aaaacaaatt gatatattat ctaggtcatt aaaactttta tcttcttgtt tatctaaatt
     6541 gaaattagat atattgccca atttactcac tccttttcaa aatattctgt atttaatttt
     6601 tatcttccaa ctagttcatc taatgtaatg tctaaatagt cggctatttt tattaatgta
     6661 tctatagttg gatttttatt ttctcctctt aaaattgcat ataaattccc tgaatctaca
     6721 cctatttctt ttgctaattt ccatgctttt aaatctctat cttttaaaat tttatttatg
     6781 ttgtcattaa ttgccattat tttcctcctt tgatatacta tatttgtagg atatatccta
     6841 tatcttttta tgaaagttgg tgatattatg cagttcagta aagatatatt acatactctt
     6901 actttagaaa ttcttaagga aaaatatgat tttaaaagtt cttctgaaga ggaactttta
     6961 aaacattacc atgaaatctt tttgaaactt tcagaagtca ataatagttt ttctaaaggc
     7021 gatggcctca gtgtctttaa acaaatgtag gtactaaatt atatttaaga gcttctttgc
     7081 aaaaatctaa aatatctttt gaggaaagga tactttgttc attttcattc aatgtattaa
     7141 gtatcctttt agctatttgt aactcttctt ttgataatat taattccgtt tcattattta
     7201 cattgttcac tacactttca aatgaaattt tcatttaatt acacactcct ttttaaaata
     7261 ttctgtattt agttttcaaa gtgctgttat attttaactt aacattgata attgatttac
     7321 taatttcaat ttgttgataa agtatatttg acctttgcct gttacttttg gtgttttagt
     7381 aatctttgta cttccgtcag ggttatatat tgctcttttt ttagtttcca ttatttttaa
     7441 gtcaacactc ttttgagttg gtgtattgta atcttcaccc ttacgtttta ttaagtaacc
     7501 attatctcta agccatgcaa ataatctttt ctcgcctgta tcaatgccat tttgcttaag
     7561 tagttttgca agttctccaa ctaatataga atcatccgag gatgctactg catcagcaaa
     7621 caatactttt ggttgctgta actgaattac cttatctttt tcttctatct ctctgctttt
     7681 cttttctatt gtcttttgag ctacttgtaa tgctcttgcc attatttcgt cgtctgtcat
     7741 atcttctgtt gtatgtatat atcctccagt tttacgtatt gttggtaaaa cttcatcaaa
     7801 tacccaactt tcaaattttt ctgcatttgg tagtttacta tttacaatca atctatacat
     7861 atcactttca ggtattgtat ttacttctaa tatcttattt tcattttgcg gatgaggtat
     7921 gtaacttttt gttacccacc tacaatggtc atttattgct ttacttgtgt ttgcatatcc
     7981 taaacattta gctatatctg ttgcaacaaa atatggtttc ttatcaacct caaccattct
     8041 tatttgacca aattccaatt tttcaaatat ctgtagatta ttcatattta ttcctcgctt
     8101 tctacattga atttattttc tttttttatt ttctcaataa actcccaaca tgcatccaca
     8161 ataatagaat ttttactttt tccacattct ttggctatat tctgtacata ttgatttaat
     8221 tttgggacga gccttactgt cattcttact ttttccattc ttattcctcc ctccttatga
     8281 cacttattca ctgtctataa tcattatatt atgacactat tttagtgtca agactttttt
     8341 aaatattttt tgtataatgt cattagggag gtgtcaaaat gactactatt actgtaagaa
     8401 tatatacacc attaaatgaa aatttagaaa aaatttctta tcaaacaggc attctcaaat
     8461 cttcgcttat tctatacgct attaatgata ttattagaaa ttcaaaagtt aatgaacttc
     8521 aatcaatctc gtataaaagt gatgatactg ttcgttctac tcttaggatt cctggtgtcc
     8581 taaaagagtt gctagagaaa acagctaaag aaaataattt atcagtcaat tctctaataa
     8641 ataatgctgt gcattcattt tgcatatctc attggttaat ttatctttga tatatacaac
     8701 caacacgcaa ttattacaat agatgcaata ggcattccga tttccttact taaatctatc
     8761 aacgctttgt acaagtcttc tggtatacga agcgttgttt gctcgtgttt caattcaatc
     8821 acctcttttg aatattctgt atttagtttt caaggtactg ttatgtttta acttaacatt
     8881 gataattgtt tgctactttt aaatttatta atgaagtata tttgtccctt accagtaatc
     8941 ttaggtgttt tagtaatact tgtatgacca tctggatgta ctcttgtacc ttcttttgtt
     9001 tctataactc ctaaatctac acttttttga gttggtgtat tgtaatcctc acctttacgt
     9061 tttattaagt aaccattatt tcttaaccag tcaaataatc tattttgtcc tgtatcaatt
     9121 ccattttgtc taagcaattt tgctaattct ccaactagga ttgaattgtc agaagacgct
     9181 accgaatcag caaacagtac ttttggttgt tgtaactgga ttaccttatc cttttcttga
     9241 ttttctaatt gtagttgttc tttttcttca acttcgatta ataactgttg cagtgcttct
     9301 ttatatgtag ttggtaattt aggttgttgt tcttttaact cttgctccat ttcttcaaac
     9361 ttagttacat aaattgctgt aaatataatc cctttttctc ctgtcatttt attagctacc
     9421 atatcgcaac cttttttagt taataagtag caaggttgga ttttattttg agtatttata
     9481 taagtacttt ctataaagaa atcttgactc ctcaaatttg atgagtcctc taaaatcttc
     9541 ttgtatcctc ttatatctct taataaatta tcgtgcttct tttctattaa ttctgctact
     9601 tctctacttt caactaaaaa ttgattattt tgcttgatta tggttagatt cttcattatt
     9661 attgcccctt tcttctttta ttgctatttt agcaacttca tctgaaaaaa aataatccgc
     9721 agatacatta tataatttag atatcttttt tatttcactt gctttaaatt cgttttttcc
     9781 ttttaatttt aatctaaaac catatgagct aagacctaat atatctgcca catttttttg
     9841 ggtgtgtcta ttttccttca tcaatccttc taatctattt aagtacatta aatcacttcc
     9901 tttttgctat tttggcaact tcatatttat ataatatcaa gttttgtgat tatagtcaat
     9961 acttttgttg cttttttagc aaaaaaaatt gtaataatta attttgttgc tataatataa
    10021 ataaaagttg ctattttgga attaatataa aaaggggttg tgcaaattga ataggataaa
    10081 agaattgaga gaagaaaaag gcatctcgct agacaaatta agcgaggatt tacatataaa
    10141 caaatctaca ctatcaagga tagaaaatgg tttaagagaa cctaagaaaa gtacaataga
    10201 agaatatgca aactattttg atgtgtctac agattattta ttaggaagaa ctgatgttag
    10261 aaatagctta tttataaata aaaacgaaaa agattatgat gctgaaaatt ttaaaacaga
    10321 aaaggagctt attgagaata tgtatcttga cgaagatatg aaagaagttt ttaatatatt
    10381 tagcgagcta agcccagacg caagagaaaa agcattaaaa gttgcagaat tatttttact
    10441 agacgaaaaa aataaaaaat agtttatttc tgaaaatgaa tataataaaa aagaggaatc
    10501 attcctcttt tttattgatt ttatgtattt ctttcacttt tattttatat tcattgattt
    10561 tatttttatc caattctttc aactttttca ttaataagtt taactttaaa atatcataat
    10621 atttcgtctt attcaaatat atcatcccct ataaaatatt ttatttatta attccacgaa
    10681 acatacgttc ttaaaaatag ttacaaccac ctctttttca aagcttaaaa ctataaaata
    10741 actgtaaaat attattatat tttatagctt ctattatttt tgctttatct agataaagtt
    10801 atttcttaac tacattctag cacaaatttc caacaaaaag tgtgcgaata ttgcacattt
    10861 attacaagaa attacacaaa ctaacatata taaaattatc taaaaggtag gttaaatata
    10921 tgttaaaaga gttacgaaaa aagaagaaat taacacaaat agagttagca aaaagagttg
    10981 gttgccacag aagtcaaatt tctaggttgg aaaataatga ggataaagat ttaactatct
    11041 ctattcttat tgaattagaa atagctttag gattggaaga aaaatattta gtaaattatt
    11101 ttgctgatga atatattaaa aaaagaaaat tacataaata attcgaatgt ttctatcaaa
    11161 tactattttt aatatataat attatttgag gtgaataaat tgaacttaag ttttaataaa
    11221 aagaaaaaat ttgaattaag caaagaagaa ttggaattaa ttgaaaattg gttaggtaat
    11281 aaatatgcag gagatatgac gatacctgct attgttgact tttctttaga aactgatatt
    11341 gagtatgaaa aaatagttgt ttatttggca gaaaaagtgc tggaatcacg tgataaaaaa
    11401 cattaaatat gtatctaaat aaaaaagact attaattata aacataaaac tttgtttctt
    11461 atagtctttt ttatttattt tatttaaatt gatatatgtt attatataat tacataaaaa
    11521 agcttttgag aggagaaatt ttatgaaagg cggcgtaaga aaacgtggaa agaagtggta
    11581 ttactacttt gatgcaggta tagtagatgg caagagaaaa aaggtagaaa gagttggtgg
    11641 aaacactaag aaagaagctg aaaaatcgct ccgtgatgca ataaatgaat atgaaaacgc
    11701 tggtatagtg tttgatgaaa caaatatgag tttatcagac tatcttaact tttggtacaa
    11761 agagtatgta cttcttaatt gcaaatacaa tactcaggaa agttatagaa atttaattga
    11821 aaatcatata gaacctagac ttggtaaatg taagctaaaa tctataaatc cagctattat
    11881 tcaagaattt ttaaataata aatcaaaaga gacatacaca caaaacggag aagaaaaaca
    11941 ctacacaaaa ggagttttaa aagcgattta tgttgtatta aatgctgctt taaaatctgc
    12001 tgtttaccct tacaaactca ttaaggaaaa tcctgttcaa tatgccagta taccaaaaaa
    12061 tgttttaaag gtaaaaaatg agtcagataa caagactata acactagatg agttcaataa
    12121 aatactagaa atatatccta aaaatacaaa tatctatatt cctctactta tagggtttca
    12181 tacaggcatg agaaaaggag aaatattagg tctttgttgg gataatgttg atttagataa
    12241 taatataatc aaagttagaa aaaatttaat aaagagaaaa gtttcagaat ttgaattagc
    12301 atcacctaag acaaaaacat caataagaga tattaaaata ggtgatactt tgtctagaat
    12361 attaaaagag gaaaaattga atcaaaaaaa acaaaaaatt aaaattggaa aatggtataa
    12421 agaaactgag tatgattggg tttgtagaaa aaaagatggc tcatttgtaa atcataacaa
    12481 cattgatgct tgtgtgagaa caattaacaa aaaattaaac cttgacttca acttccattg
    12541 tttaagacat acacatgcta cattattatt agaaaatgga gctaatataa aatatataca
    12601 acaaagatta ggacatagtc aattatcaac tactatggat acatattcac atgttacaag
    12661 taaaatggaa agtgaaacag tagatatttt ggagggtatt ttacaataat ttgccaccga
    12721 aaaaaaatta tggtggcaaa caggtggcaa aacgataaaa aacattcttt tttttgctca
    12781 aacactgtta ttttgctatt ttatataaaa aagtcgtatt tctcacagtt atacgactta
    12841 tattaaattc tttacataag tctccttcag atggaagttt atctcctggt ttatatatgc
    12901 cagatgctat ttgttttttt atgttgtcat atagttgttg atataaagga acaaaggaat
    12961 ttacttctaa atccatttct tttttttgtc tcttttcatc cattttgctc acctccctat
    13021 gtaacatttt tataacatta tataacaagt tctttattac atattataac aagatgaaat
    13081 cgttgtcaat tttttttaaa ataattttta atttgacagt attttctttt attttctcta
    13141 gtgtgacaaa ttgctatttt tacataaaaa taagactgtt gtacaacaga ttaaattgtt
    13201 tatacaacag cctttattca cttattacat ttatattttt acttataaat ttatattaaa
    13261 aaatgcctta tatcccttat acatttcata tatgtctact ttagagacaa tctcatgtgg
    13321 tgcatgcata tttaaaactc caacaccaca gtctataacc tctgcacctt gatttgccaa
    13381 tatatgggct atagttccgc ctcctccttg gtctactttt ccaagttcag cagtttgcca
    13441 tactacattt cctttattaa aaattctctt tacttctgac ataaattcag cattagcatc
    13501 attacatcca ctctttcctc ttgaacctgt atacttagtt aaaactactc catttcccat
    13561 atatgccgaa tttctctttt catatgcttc tccaaaattt gggtcatatc cagcagaaac
    13621 atcagctgat aataccttac tatttgctaa agctcttctt actttaatat cacaataatc
    13681 tccttctaaa gcaatcagtt cagccacagt attttcaaaa aactttgagt gcatacctgt
    13741 attcccttta gaaccaattt cttctttatc tacacataat gcaactgaag taaattctgg
    13801 ttgttcagtt tctagtatag cttttacagc agcatatgaa cataccctat catcatgccc
    13861 atatcccaac accatagacc tatcaagccc taaatctcta gccttaccag ctggaactac
    13921 ttcaatttca gcacttaata aatcttcttc aactatattg tatttctcat ttaaaatctt
    13981 taatatattt gctgttatag gttctttttc ttcatcttct aaaggcatat tcccaattaa
    14041 tatatttaaa gcttctccac taattccttc tgatagtttc ttttgcattt ggtctccagc
    14101 caaatgaatc aataaatcag ttacacaaaa tactgggtca ctatcttctt ccccaataga
    14161 tatgtccact tttgttccat catttaatat aactatacca tgtaaagcaa gtggaatagc
    14221 cgtccattgg tattttttta ttccaccata ataatgagtc ttaaaaaaac ctagattact
    14281 ctcttgatac agtggatttg gttttaaatc taatcttggc gaatctatat ggcttcctat
    14341 aatcttcata cctttttcta tataattttt acctattaca aaaagtgcta ctgccttctc
    14401 tttatttata acataaattt tatcaccaca acttatgctt cctttttcca tagcttcatc
    14461 tattgaaata tagttatttt ctttagccaa ttttataatt tcttttactg aaagtctttc
    14521 agtctttgac ttacttaaaa actccatata attgctagaa tattgcataa tttgttcaat
    14581 agtattttct tttctttcaa aatcccaagc attcttaaat tcgtacttta agttcatatt
    14641 ttcctcctac taagttctaa tttttattgt ttttcatgta ttgctcatat tcttctggtg
    14701 aaactacttt gacactatct agtcttgatc taaagtgggc tctaaagttt tctaaatatt
    14761 ctttccttaa aatttctctt tctttgattt catcagcaga aaggattccc tctttatttt
    14821 tctttgctaa ttcattaatc ctatctaatt tcttcttgtc aaacattttt tcctcctaaa
    14881 aaacctctat ttatttaata aaataattta ttgcttttta tatctaactt gtttttatat
    14941 tcacaaaaaa actcatctat attatcactt attatagata ttcccttttc tagttcttcc
    15001 atgcttggac gagctatatt tatcctaaag aatctatcat ccactagatt gtcaaaaaaa
    15061 taagtccctg gaagcattga cactcctttg tttaacataa aatttgtaaa tgcttgagat
    15121 gtatatcctc ttggtaattc taaaaaaaag tttattcctc cacaagattt tctaactttt
    15181 aatttaccac tcaatttgtc attaatcaag ttttttgcca atttatactt atcagtatat
    15241 atcttctcta tatgactcaa ataattcttc caatcaaaat tattcatata gtaatacatt
    15301 gatttttgaa ttaaacctgg tgttgatata tctgatgaat atttagccca taaaactttt
    15361 tttaacaact catttggcat ttctacaatt cctatcctaa gacctggcat aagtattttt
    15421 gaaaagcttt ttatatatac tactctattt ttatcatcat aacttctaat tgttctattg
    15481 tcctttgatt caaaaacaaa atcacttata aaatcatctt ctattatata aaaatcatgt
    15541 tcttctgcca attctatcaa tttctttttt ttatatgttg agtaagatat tccagttgga
    15601 ttttgaaaat taggcataat gtatataagt cttggtttta ttttttccaa ctttaatttt
    15661 aaaataccta tatcaattcc atcatctaac ataggtatag atattatttt tgcacctcta
    15721 cttttaaaca cttcaattgc tccattgtaa gaaggttctt ccataaaaac aacatctgaa
    15781 taacttataa gtcctttaca aactatatct ataccttgtt gagcacctga tattatttga
    15841 atattttcct tagtagtttt tatatttcca tcacttagat actctaccat tttttctctt
    15901 aattcttcag agccaagacc ttcatcatat tcaaatattg aactaccatc tttagataat
    15961 gccatattta cagcattttt aaatgaatcg ataggaaaca tatcattaga tggattccca
    16021 ttgtcaaaat gaattaaatc ttctctttgc acaataatct tatttttcct gttatctata
    16081 ccgcttttat aagaatttaa acttgataca aaagtaccac tccctataat tttatataca
    16141 tatccttcat tttctaaaag ctcgtatacc ttaactatag tagtgttatt tacttgtaaa
    16201 aaatttgata gttttcttat tggaggcaat ttttcatgtt ctactaactc tttatttatt
    16261 atcaattctt ttatatgatt aaatatctgt atatatttag ttgatttttt ctcatttaaa
    16321 tctattctgt atttttccaa atttatcacc gctcattgta tttatatttt attgatgctt
    16381 ttatgtatat ttgatattat ttagtatgta tatataatac caaaattttt ataaaaaggg
    16441 gaaatattta tgttttacga ctatcacatg cactcaagtt tctctacaga cggaaaatct
    16501 acaatggaag aaatggtaaa aaaatctatt gaactcggtt tggaggaaat ttgtttcaca
    16561 gaccatgttg actatgatgt atatgctgat gattcttttt caattgtcta cgaagattat
    16621 tttaagagtt tagagactct tcaaagcaaa tataaagata aaatatcaat aaaaaaagga
    16681 attgaatttg gagttcaaac tcaattaatg gatacataca aaaaggaagc acatcaatat
    16741 ccattggact ttataatatg ttctatccat gctatagata caatggactt ataccttggt
    16801 aattatttta aagataaaac tcaacatgaa gtttatgaaa attactatct ttatttatat
    16861 aatattgtta agaattataa agattactct gtcttaggtc atttagattt gattaaaagg
    16921 tatgcacctt atgacactat tttagatgat agactatttt ctgatataat tgaagaaact
    16981 cttaaacaag ctatttatga tggcaaaggt atcgagataa atacctcttg ttatagatat
    17041 aatcttccag acctaacccc atctaagtat atacttcaaa tgtacaaaga cttgggaggg
    17101 gaaatcataa ccacaggttc agattcacat catataagtc aagttgcatg tgagtttgac
    17161 tacatatatt cattactaaa aaatatggga tttaaatatg tatctaaatt taataaattg
    17221 aaaccagaat ttataaaatt ataaattttt acacctcctt gtgaatatat ttaaaagata
    17281 aggaggtgat ttttttgaaa gtaccttatt tagtactatc aacagttatt ttatttatat
    17341 ccaattttat tgtaagaata tttggttttt tatataaaat attcttatca agagcactag
    17401 gtgaaacagg tttaggtatc tatcatatga tttttaactt tttgatgatt tgcttagctg
    17461 ttacaacaac aggtatacct actgcactta gttgtctagt tgcaaagaga aaagctttaa
    17521 acgatagaca caatactaat gctttattta tatcaacttt atatatatca tttttcgtag
    17581 cactaattat atctatagtt gcatctttca atagttcttt tttatcactt aaatttttaa
    17641 aagatgctaa attaaatcta tttatactag ctgtttgtcc tgctatagtt ataataactc
    17701 tttcaaacgt actaagagga tattactatg gcataaagaa tgtaaaaatc cctgctattg
    17761 gacaaatcat tgaacaaatt ggtaaaatat tatttgtttt tttacttgtt atgtacataa
    17821 ataataaatc tatgaattgt tatatagctc tattaggtat atcgattggt gagttgagta
    17881 atattatatt tatgcttatt tgcttatggc gagattcatc atttgacaat aggtatatta
    17941 taagcataaa agatttttat aattcttcta tggaaacatt aaaaatgtct attcccataa
    18001 cttgtaatag aatgtcaaac atacttttgc aatctataag ttctatgatg ataccatcaa
    18061 gattagcatt atcaggaatg acttatcaac aatctttaag tatgtatggt attgtcagtg
    18121 ggatggtaat gccttttata tttttacctt ttactgttgg ttcagcgctt attgtaaatt
    18181 taataccaac tatttcacaa gaaatggctc taagaaagcg taagagtgta attaaaaaaa
    18241 tcaaatactc catacttctt actttatttg taggcatact atcatcaata tttttttatt
    18301 tctttggaaa agatttatgt atattagtat ttaaaaatac acttgcagga gaatatctaa
    18361 aagctatgtt tttagttcca ctatttatgt ccctaaatca aactctatct ggcattttac
    18421 attcaataag aaaagaatta gcttcaagta taaatactat tactggcatg ttaattcaac
    18481 taatagcttt atatgtattt cttccaattc caggacttaa tatttatgca tacatataca
    18541 caatgacgat agtatcaatc tttacttgtc tattacatac tatagtgctt tttaaatcac
    18601 taaaatctat acgctagtta taaaaacgtg aaggctacca atgtaaatta tacactttgg
    18661 tagccttcct tagatgattt ttatttataa tttaaactgt ctatatatct acctatctat
    18721 ctgtggcaac ttggctttcc acttccatga ttaggtccac attgcgaaaa atcacatact
    18781 ttagagaaag tatcaacaag tcttggttgg attggatttg gtgatgctaa aactactatt
    18841 tgttcactta ttaatgtgct aactgtttct tgtaagcaaa gtactaaatc tatatcattt
    18901 ggtataatta attcatagaa aggttcacaa cattcgttgc attctaattt taatatagat
    18961 tttcctatac aatctacagt taagttagtt tgatattctt gtcttaaagt aacagctctt
    19021 ccgtcagctg gcaagcatat tggtgctgaa agtgtgttaa aagtaaattc tacataatca
    19081 actcttctct tacatccacc tcctgctcta actgctggga aagctaatgc aacgaactct
    19141 gtacatccac atctacctct tagctctagt actaaattac gaactgctac agttaatcct
    19201 ctttgtttat aagctactga tttgcctttt ccttgtctac agcactcaga gtttctttct
    19261 ccacaaactg caaattcaaa agttgtttca cagtttcttc ctactatatc tcctaatggg
    19321 tctaaatcga aatcttctaa agttgtcatt cctgtgtcta taacgattcc atcattactt
    19381 aagcatattt tttctatagt tacacttgct tgacctgcac ttggaactga accaaatact
    19441 tctactactt ctgtttcaaa agttaatggc tctccatttg gtcctgttgc atctgtaaat
    19501 gtttggaatt gcatagtatc aaatattcta ttagcctcta ttgcaaatgg cactgcattt
    19561 gatacagaga agtttcttgg agatatagga ttacagcatt ctggtccaca tttgttttca
    19621 aaacattcat ctgaatccat ttcacatggt tcacaatcac atttacaatt gtcatggcat
    19681 ccacaattgt catcacatgg attaggtttg catggattgc atgggttagg tttacatgga
    19741 ttacatgggt tgcatggatt gcaatcatca tggcactcac acttattatc agtattacat
    19801 ccattatgat ggtgtgattt tttaaaacct ctcatgtctt ctgaatttat ttcatctgta
    19861 tacacttctt cttcattcat tgtagacatt ggctgattca tcattcttct tttatttttt
    19921 ttataatctt gcatttttca ttgccctccc tgtaagcgat tcttattaag tctttggctt
    19981 tttgaagcct aattaatact atgcttttat aatcttaatt gttacttgct ttttgaattt
    20041 atcaataatt aggcgtattt agtagaaaaa tgcatattaa taaaaaaagt actactattt
    20101 gaaatagtag cacttttttt gtcccgaaac accggtggct aagtaaattc acaccctagg
    20161 cactcccagg tgggtgactt gccagtaacc tttaacgtcc aaactaacta ggcatgttat
    20221 aatttaccag acacggtctc ccgtatttca tttgtaggtt gaatttaaaa aagccttatc
    20281 gtatgtttca ggatttcata ttattattat ataataaatt ttgaattttt taaagtggtt
    20341 ttatatagta attgagataa tcttattcta tcattacttt ttcttccttt tttctttatg
    20401 tatttagtta attatcgttt tttcttagct ctagttttag tatttttttc attataattg
    20461 tattcggatt cttgctcatt aatgttgtat agttcctcat cgtatactat atcttcatca
    20521 ttttctatat tttcatagcc tgtgttctta tcttctacta attcatcttc attatcctcc
    20581 atttcacctt catctgttaa ctcatcatca tccaattctt tgtcttcaca atctaattct
    20641 tcgtcttcat aatttaactc atcattattg tcttcatcat cattattact atcatcacct
    20701 cttaaaagac ttcctaacaa tttgagatta ttcatactac caagattgtt tatatttgcc
    20761 aaagagctca aactaaatcc accttcacca aagtcatctg gtaaaaataa ttctgcaaat
    20821 aaatctatct tctttttcat tcctatagct ttttcaactg aattttttct tttttctggg
    20881 aatacatcta taatttcctt tatcatcata ttctttttat catcactatc catttttctg
    20941 aaaaaatccg tttcatcttc tgtatctacc atatctatat tatttaattt ttttattcct
    21001 ctaactaaat ctaaaacact ttctattttt attagtattt ttctttcatc tcttgacatg
    21061 tattttttag ttcttgttat tatttccatt cccatttcta aatcttcttc acttaaatcc
    21121 atgagcctta aacctctttt tatatcattt gcatcaaacc ttaaattgcc atgtgtagac
    21181 tgtgaaagtt tatcatttgc attttttttg ttttttctaa ctctattatt tccttttgat
    21241 ttatttttat catcaaatat agagatatta ccattactca atgcaatcac tccaatcaaa
    21301 aatagtaagt tattcaagac aattccccct tatattatat aattgcccta tattaaagta
    21361 tgtttttcat attttaatgt tccattttta aagctcagtg catatatatt atctataaga
    21421 atttttattt atattatata tcactcacaa ttcaccactt caatattatt tctatatata
    21481 aatagtatga atataattca atatatattt atagaaaggg gtttttttat gaaaaaatta
    21541 gatacagagt cattagaaaa aatggctaaa caaaaaaata ttgacaagga taaaatagaa
    21601 aagatggctg atagttacaa aggaaaatca gaaaatgaac ttatggagga acttataaaa
    21661 ataggtaaga atctagatgg cagagatgaa gtagtatcaa aattcaaagc atttttagat
    21721 gaaaatcaac gcaaaaaact ggataatata atggaaaaaa tctcagatgc agaaaaccaa
    21781 agagatacta aaccagctaa aactaagaag gctaaaccta ctaaaggtaa gtcaaattca
    21841 cctgcaccaa aaaatacacc aggtagtcaa caaaaaaaat ctaaaagttt atttaagaaa
    21901 actaaaaagt ctaatcctca tgaatagata aaaataaact atttaagcaa ttagataatt
    21961 atttttagtt aatctattat taaatttgat attattgaca taaaaaatca ggtagtgcct
    22021 aaggcactac ctgattgatt ttaatagttt tatttttttc tttctgtaac actttctcta
    22081 tcaacaattc tataaggtaa ctctattttc ttattttcaa ttaattcctt atttattaat
    22141 tttataacca ttcttattgc tactgctccc atatcgtata gaggttgata tactgtagta
    22201 agtttaggtc tatacattct agctatctta acatcattaa atcctgcaac tgatatgtct
    22261 tctggaacct tatatccagc atcacatata gcatttatag cacctatggc agcctcatca
    22321 cctgttacaa aagccgcatg aggtattatt ccatcatcta atagttcttt catactattg
    22381 tatccactct cataatcagt tccaccatac ttaatcaaac tcttgtctaa ttctatattg
    22441 ttatttgaaa gtgctttctc ataaccagca agtctttcca tttctaaaac agtatcatct
    22501 ttactagtca ttataaaagc tatcttttca tgacctttct ttataagatg ctctgtcata
    22561 tcaaaagtag cttcttcgtt gctagtactt actgtatata tatcataatt tctagcagtt
    22621 ttacttatat atgtcgctgg tattccacaa ttttgtatat agttgatatg ttcttgttca
    22681 actatccatg aaatcataac tataccttcc acttgttttg ctctcaatag atttatactc
    22741 ttaagttctt gttccttatc agagtatgta ttcgctaaaa gaatatcata gtcatacatt
    22801 ttagcaacct cttctatccc atttaacacc tcattaacaa aagaatctga aacttctgga
    22861 actattaccc ctattaattg actcttcttt gttactaagc ttctagcaag tggatttggt
    22921 atatatccag tctcttttat aacctctaaa actttttgtt tgacttcatc agttacaggt
    22981 tttgaatcat ttataactct agatacagta gatattgaca ctcctgcttg tttagcaaca
    23041 tcttttatcg ttatattgcc tttcatcttc atcctcctta ttttactaaa tttatctaaa
    23101 actgaaccaa atttttgaag gttcctattt taactacatt tatataagtt tttattttac
    23161 cataaaagca agtacattta aatacattat attttatatt ataccctata ttcaacaaat
    23221 ttaataatat tcattcttaa tttaaatcat attaaaaaat aaaataaaaa aatgctggta
    23281 taaaatacca gcattttttt attctaatga tttaagtatt agcagccgca acctacttca
    23341 ccatcaaaga atccaccaca tgaacaagtt attagcaata atattaagaa aggtatccat
    23401 gacattatgt caatgtctct ccagcaatct tcaaaagcta agaataggaa gaatagtact
    23461 attatccacc atcctcctcc accaaagcat ccttcttctc caaaaaatct atctaacata
    23521 acaaagccct ccttttttta ataactgtgt tcttattcta agcatccacc acagcagcaa
    23581 agtattaata aaactattag gaatggtatc catgccatta tgtcaaattc tgcccagcta
    23641 tctccaaaag ctaagaatag gaagaatagt actattatcc accatcctcc tccaccaaaa
    23701 catccttctt ctccaaagaa tctatctaac attatacgtt acctcctgtt gatattttgt
    23761 tgagcttttc gctcctaaca tatactatgc atttggtttc cattttgcta atgtattttt
    23821 actatttttt taaatttttt aatttgaact aaaaaaggta aaaccataac ttgtataagt
    23881 tactggtttt acctatttaa taatcaatat tttttagcaa caacaaccat catcgaagtt
    23941 aaataataac aatactatta atataaccca tatccatata ttgtcatcgc ataataagtc
    24001 ttctaaaatg tctgtgcata atagtaagaa tactataact attattatca acattttatt
    24061 cccaaaaaat ccttcgcatc ctccaaagaa acgttccata ttgtcttcta attttgtcat
    24121 aactaatcct ccttgaatca ttaagagact aagttttgtc tctttaatta aatatatgta
    24181 gtaatagaca gataagttac ttctaaaaag tttttattga ttagtttatt tatttctttt
    24241 tcaccaagtt tattgtatct atcaacttca tccagataat cttttttctc ctctgatata
    24301 gtatagatta aactttctat tgattttatt ttgtactcct tatatctttc tatcaagaag
    24361 gatgtttttg aattttctat tcctttcttt acatgcatta accttgatat gtaatcttct
    24421 ttttcattag atacataact ctccttttca gctacatctt caaggattaa attggtctcc
    24481 attacataat cctctatacg ttgcatatct aaagttattg aattataata aaaatttact
    24541 gctataagta aaaacattcc tactgaagtt agtaatagtc ctacatattt catagcattt
    24601 cctcctaagt tatatttgtt atcaataaag tttattcatc aaataatact ttattactac
    24661 atttataaaa ttttattatt ttttatagat tccatattga aaaattcttg atattatatt
    24721 aaactaacat taaaattaat taaaactgct ttaggaggct taaaatgttt gagaaaataa
    24781 aagaaataat agcagaacaa ttaggggtag acaatttaga tgagataact atggaagcat
    24841 ctttaatgga cgacttagaa gctgattcat tagatgctgt tgaaattata atggcccttg
    24901 aagatgaatt tggaatagaa ataccagacg aggaagctga aaactttaaa tgtattggtg
    24961 atatatgtaa atatatcgaa gaaaacaaat agtatcgttt tgtctacaaa tataaatagt
    25021 ctgtgtaaac ttctacataa taaattatat tagagttcgc acagactaga ttacatttat
    25081 ttacaatata taaaatacac gctcttatat ttaatttata tactaagtat ataaattact
    25141 tttcttcata aaacgtaaca ttctttatta ttatatctat agttccatct tgattttgtt
    25201 ttacagtata cttcattggg tcttcaaaat cagttagatt cccttttatg tcaaaaccat
    25261 tatccgtttt tatacttctt tttttaagct ttttctcaac ccatttttta tctatactaa
    25321 aactttcgtc aagacctttt tcttccatat gctctttaaa gctatccttt aactcatcat
    25381 ctttaattga attatcaaca aagtcattta tatcaacttc atgtttttcc ttcagtgtat
    25441 aatttaatat acttcttaca tcttcagctt gctttatatc attactaaga gcattggtta
    25501 tccaattttc agctgtattt ttaaatacct tggtcttgta cttatcatca tctatttttt
    25561 tagcatttaa aaattctgtt acaaacttag attctgaccc ttctttttca gcatccttat
    25621 ctaaaagcct tagatgaaac tcatcattta taccacttag actaatcaaa gctccttgct
    25681 tttgtcttaa agtctctggg ataccaattt catttgaaac catttgaata ttaaatttat
    25741 catccacaaa ttcaattgaa tgagtgtaca actttttata atctaatttc agtattgcta
    25801 cactcttttc atctttaata gtgtataaac aaattgccaa atcacaagat tctagtgcag
    25861 cattaatttt cataacgtca aacaagtaag atgcaatttc tttagaattt ttcaaaaaag
    25921 tactttcatc atatattatt tgttcacaac agtttttaat taaattgtca ttataatctt
    25981 taaaaacacc ttttcttaaa tcctcatctt ttgcaattct ttttatagct ttctggaaaa
    26041 aaccatccac ttcttgatta actttgcctt caaagtcatt taatattgga acatcactat
    26101 ttttgtccaa aacatgtatt ataaatttat gtattatcat atatatcacc ccattatctt
    26161 aatttcgata ttaactcttt catatcctct ggtatatctg ctttaaattt caatttttct
    26221 tttgttcttg gttgttcaaa ctctagctta tatgcatgaa gtgcttgcct atttattagc
    26281 tcttcatcta cataaccata caactcatca cctattattc catgacctat atgagccata
    26341 tgtactctta tttgatgtgt tctaccagtt tctaagctta cttctaatac agttgcattt
    26401 tctagtcttt ctattacttt atagtgagta acagatgact gtcctctttc atctattatc
    26461 ctctttattg aatcttctgt aggtctataa attggctcat ctatagttcc ctcattttct
    26521 tttataattc catcaactac tgttatatac tttttttcaa ctttattttc actcattgct
    26581 gtagacaaag tatgatgtgc ataagcattt tttgctacta taactaaccc tgaagtattc
    26641 atatctaacc tatttacaaa tcttattttt acgttttctt tttgattatc tatatagtaa
    26701 ctaataccat ttgctattgt tttatcatag tggctttttg ttgggtgaac taccataaac
    26761 ggaggtttat tcaccataat tatatcaaaa tcatcataaa ttatctgtaa atttaaatct
    26821 tgaggttcaa aatttgcttt ttcctcgtct atttttactt ctattaaatc accactatac
    26881 acctttaaac ttggtttttt atatactcca tttactaaaa cactcttttc cctcttcatt
    26941 tttgataatg acctaactga gaaatttagc ttgtccaata aaacttcttt taaagtcatt
    27001 tcttcttcat ttgtatacga tataagatta tatctttgat tctctttttt aaacaaactt
    27061 ttacctactt tcttttatat cttcctactt tttatatctt caaaatcatg atattatata
    27121 ttt
//
LOCUS       DAMJYC010000050        26615 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJYC010000050 DAMJYC010000000
VERSION     DAMJYC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.051"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(157..300)
                     /gene="scfA"
                     /locus_tag="PC472_002281"
     CDS             complement(157..300)
                     /gene="scfA"
                     /locus_tag="PC472_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2727600.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(366..728)
                     /locus_tag="PC472_002282"
     CDS             complement(366..728)
                     /locus_tag="PC472_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2727601.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGFLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(786..1079)
                     /gene="yajC"
                     /locus_tag="PC472_002283"
     CDS             complement(786..1079)
                     /gene="yajC"
                     /locus_tag="PC472_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2727602.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1213..2334)
                     /gene="tgt"
                     /locus_tag="PC472_002284"
     CDS             complement(1213..2334)
                     /gene="tgt"
                     /locus_tag="PC472_002284"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2727603.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2413..2898)
                     /locus_tag="PC472_002285"
     CDS             complement(2413..2898)
                     /locus_tag="PC472_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727604.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2910..3935)
                     /gene="queA"
                     /locus_tag="PC472_002286"
     CDS             complement(2910..3935)
                     /gene="queA"
                     /locus_tag="PC472_002286"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2727605.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4253..5272)
                     /gene="ruvB"
                     /locus_tag="PC472_002287"
     CDS             complement(4253..5272)
                     /gene="ruvB"
                     /locus_tag="PC472_002287"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2727606.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5379..5981)
                     /gene="ruvA"
                     /locus_tag="PC472_002288"
     CDS             complement(5379..5981)
                     /gene="ruvA"
                     /locus_tag="PC472_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2727607.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5974..6483)
                     /gene="ruvC"
                     /locus_tag="PC472_002289"
     CDS             complement(5974..6483)
                     /gene="ruvC"
                     /locus_tag="PC472_002289"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2727608.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6795..7424)
                     /locus_tag="PC472_002290"
     CDS             complement(6795..7424)
                     /locus_tag="PC472_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727609.1"
                     /translation="MFEKKKKELFPWKISALILIGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7627..7950
                     /locus_tag="PC472_002291"
     CDS             7627..7950
                     /locus_tag="PC472_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2727610.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8218..9072
                     /locus_tag="PC472_002292"
     CDS             8218..9072
                     /locus_tag="PC472_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727611.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9258..10694)
                     /locus_tag="PC472_002293"
     CDS             complement(9258..10694)
                     /locus_tag="PC472_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2727612.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10765..12147)
                     /locus_tag="PC472_002294"
     CDS             complement(10765..12147)
                     /locus_tag="PC472_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2727613.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12283..13188)
                     /gene="garR"
                     /locus_tag="PC472_002295"
     CDS             complement(12283..13188)
                     /gene="garR"
                     /locus_tag="PC472_002295"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2727614.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13584..14255
                     /locus_tag="PC472_002296"
     CDS             13584..14255
                     /locus_tag="PC472_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727615.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14358..14906
                     /locus_tag="PC472_002297"
     CDS             14358..14906
                     /locus_tag="PC472_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727616.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            15168..15476
                     /locus_tag="PC472_002298"
     CDS             15168..15476
                     /locus_tag="PC472_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727617.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15638..17428)
                     /locus_tag="PC472_002299"
     CDS             complement(15638..17428)
                     /locus_tag="PC472_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727618.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17428..19155)
                     /locus_tag="PC472_002300"
     CDS             complement(17428..19155)
                     /locus_tag="PC472_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727619.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RSKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19400..19666)
                     /locus_tag="PC472_002301"
     CDS             complement(19400..19666)
                     /locus_tag="PC472_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2727620.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            19964..21076
                     /locus_tag="PC472_002302"
     CDS             19964..21076
                     /locus_tag="PC472_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2727621.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21440..21949)
                     /locus_tag="PC472_002303"
     CDS             complement(21440..21949)
                     /locus_tag="PC472_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2727622.1"
                     /translation="MSQQNTGNAIVNSKPKKTSVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(21951..22598)
                     /locus_tag="PC472_002304"
     CDS             complement(21951..22598)
                     /locus_tag="PC472_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727623.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(22612..23784)
                     /locus_tag="PC472_002305"
     CDS             complement(22612..23784)
                     /locus_tag="PC472_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2727624.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            24052..25428
                     /locus_tag="PC472_002306"
     CDS             24052..25428
                     /locus_tag="PC472_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727625.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(25678..26511)
                     /locus_tag="PC472_002307"
     CDS             complement(25678..26511)
                     /locus_tag="PC472_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2727626.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     ENIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIALLIIGVYKLFKKY
                     N"
BASE COUNT         8979 a         4527 c         3132 g         9977 t
ORIGIN      
        1 tatttttgca taattaaacg ataagaaatc aaatttaatt ttacataaat agaatatata
       61 tttttatata ataattgaaa ataaaagagg atacctcata atgataaaag aatcactttg
      121 agataccctc ttacaaacaa ctaaatcaat aattttctat ctttcacact cttgatttgc
      181 aactgtacaa gaagttttac aagctgattg acaagaagtt tgacactctc cacatccacc
      241 ttttgcagca ctttgcttta aagtagcttg agataaagtt tttacatgtt tattttccat
      301 tttcaacgcc tccatttcta attgtttcct attgattata tcataaaact actttaatat
      361 aaactctatt taaagttgac acccaatacg cctccaatgc ctcctgctgc tgatattaac
      421 aataacttat aaatcatccc tacttcaaat acaaactttt cttgtgctaa aaacacagtc
      481 aaaaaaatac atactatgta caaaagtcct actaaaaacc catacataag tcctttttct
      541 ttcatgtgtt ttgaagtgta aaaccctcct atagctgcag aaatagtagt tataaatgat
      601 gagaccatag ttatattatc tccaccaata tctgtaaatg tcaaaaataa gttatacact
      661 aataaaacag ccaaagttat tatatatgca tatcctagac ctttaaaaat ataattagat
      721 tttcccataa aaaaacctcc tctcaaatta atatatattc gagaaaaggc ttttaaatta
      781 ctaatttagt cttcttctat ttcttctttt ttagatttaa cagttcctat agcccatttt
      841 tcaaatggaa ctttagttct atttggtcct aattctaata ttacaacatc atcttcaact
      901 ttagctacat tagctactat tccacctatt gtaatgacct tgtctcctac acttaggcta
      961 cttctcattt cttttaattg tttgtctttt ttcttttgag gtctaatcat taggaaataa
     1021 aagattgcga ttactactac ccatagacct atactcataa ttatttgttg ctgtggcatt
     1081 aatttttcac tcctttgtaa attatactct tttgtaaatc ataataaatg tttatataac
     1141 aatttacttt ctctatcttt gagtactatt gaatattata tattaaaatg attataatta
     1201 atagtgcaaa acctatattt catatccata ttttgcaaaa aattcatttc taaaatcaag
     1261 taatctatct tccattatcg cttgtcttat ttgtttcatt aaatttaata aaaagtgaag
     1321 attatgagta gttattaatc ttgcacccag tatttcattt gcttttataa ggtgtcttat
     1381 ataagctctt gaatagtttt tacaagcata acaatcacat tcagggtcaa gtggagtaaa
     1441 gtcttcagca taagttgcat ttctaactac tactttacct tggctagtca ttgcagttcc
     1501 atttctagcg atacgagtag gtaggacaca atcaaacatg tctactcctc ttataacacc
     1561 ttctactaag tcatctggac taccaactcc cattaggtat cttggtttat cctttggcat
     1621 aagtggagtt gtatgctcta acacgtcata cataagtggt tttggttctc caacactaag
     1681 tccaccaata gcatatcctg gtaaatcaat attagttatt tctttcgctg attgttccct
     1741 taagtcttta tacatcccac cttgtattat accaaataaa gcttgcttgt cggtattatt
     1801 atgggcatct ttacatcttt ttaaccatct tgttgttctt tctaaagaat tttttacata
     1861 ttctctatct gatgggtatg gtgcacactc atcaaatgcc atcattatgt ctgaacccaa
     1921 tgcattttgt atttccatag ctttttctgg agttagaaaa tgttttgaac catctaagtg
     1981 tgacctaaat tctacacctt cttcttttat ctttcttaaa ggccctagac taaatacctg
     2041 gaaccctcca ctatctgtaa gtattggttt atcccaattc ataaacttat gaagaccacc
     2101 agctcttttt ataagttcat gacctggtct catatataaa tggtatgtgt tactaagtat
     2161 tatttgtgaa ccaatctctt ttaattcttc tggtgtcata gattttacag ttgcttgagt
     2221 tccaactggc atgaatattg gtgtttcaat aatcccatgt ggagtgtgta atcttcctaa
     2281 ccttgcacca ctctgcttac aagtctttat caattcgtat cttactgcgt acataaagtt
     2341 ctccttaatc atatttattt ctcattattt cttaagatat atttgtaata agctattcat
     2401 ataatttatt gtctaaatag atttatcttc gtcttctttg ttctgttgtt ccttaattgc
     2461 ttcttcatca tgaatactca ttatattaag agcactcttg tcttttgtta ctaaaaactt
     2521 ttttatagca aatagtcctg ctatagctat aactcctatt aaaacatcta tcataccttc
     2581 atttttaggc agtagcaaca tttttctagc aattgcgtaa agtaatgcct caataaatgc
     2641 tcctggtata tgaagagata gcattactac aagttcaaca cctattacta aaagtaatat
     2701 ttgtgctaaa aaatcattaa gctgactgta ttgtactgga ttttgaaaat caactatata
     2761 tgctccccac ataagtctta gaacatctat agttcctaaa aatactgcta ttagaactac
     2821 tactgctaaa acacgttcaa atataaaagc aatctttaat atagggttat tctttttttg
     2881 tttttgtaaa ctatgcatcc attacctccc tactttataa tcatagcatc tccaaaactg
     2941 aagaatctat atctttcttt tactgcctca ttatatgcat ttaaaacatt atcctttcca
     3001 caaatagcac ttactaacat tattagtgta gactctggta agtgaaaatt agtaataagt
     3061 ttatctaaaa ctttaaattt ataacctgga tatataaata tttctgtcca tccactagtt
     3121 tctttcatct ttccatcctc attacaagct gattctatag ttctacaact ggtagtacca
     3181 acacatatta cattttttcc attttccttt gctctattta ttttgtcagc tgcttcttgg
     3241 ctaaccatat aatattctga atgcatcttg tgatttaaaa catcctctac ttttactggt
     3301 ctaaaagttc ctaagccaac atgtagagtt acaaaggcta tatctacacc tttttctttt
     3361 attttattta acaattcttc tgtaaaatgt aatcctgctg taggtgctgc tgctgaacca
     3421 ttatgctttg aataaacagt ctggtatctt tctttttcat ctagtctagc agtgatatat
     3481 ggaggtagtg gcatatttcc aagttcatcc aatatttctt caaaaattcc atcataatgg
     3541 aattttataa ttcttgaacc ttcatctgat aaatctacaa cttctccaat tagctttcca
     3601 tttccaaaag aaaatttagt ccctactttt gctctttttc ctggttttac taaagcctgc
     3661 caagtatctt cctctgttct ttttaataat aaaaactcta ttttacctcc agtttcaagc
     3721 ttctctccta ttaatctagc tggtataact ctagtattat ttaatactaa gcaatctcct
     3781 ggatttaaat attctattat atctctaaat actttatgtt caatattacc tgtctcttta
     3841 tctaatacca tcaatcttga gctagctcta tcttctatag gaacttgagc tataagttcc
     3901 tgtggtaagt caaatttaaa atcacttgtt ttcaatgtta aacttcctct cttaaaattt
     3961 ctccagctac aataatatca aaaaaacaac ttattttcca caatattacc ataatctatc
     4021 aagaaattgc tatttttaac agacaccata tccacaatat atagatattt ttccacgata
     4081 tacatgtctt atccacaata tatagataca atacacacaa tttttatcgt gtttatccac
     4141 agtatctaca gatataatta ccattttgtg gatatgtttg aatttttagc cattttagaa
     4201 atattttaat ttaatgactc ttttatttta taaatactcg tataatctat ttttattttt
     4261 cattaggata tggaattttt aagtgttcat atgctaatgg catggctact cttcctctag
     4321 gacctctatt tataaatcct aattgaagca aatatggctc atatacatct tctattgtat
     4381 ttctatcttc tccgatagaa gctgccaatg tatctaaacc cactggtcct cctctaaatt
     4441 tttctattat agtcataaga agcttctcat ctacaaaatc aagacccaaa ctatcgactc
     4501 ctaaaagttc cagagcatct tttgccactt tatcagttat ttttccatca gctctgactt
     4561 gtgcaaaatc tctaactctt tttagtaatc tatttgctat ccttggagtc cctcttgaac
     4621 gttttgcaag ttctaatgct ccattagact gtatctctgc atctaagatg cttgaagacc
     4681 taagtactat ttttgaaagt tcatcaactg tataatagtc caatttacaa ataacaccaa
     4741 atctatctct aagtggattt gtaagcatac cagctcttgt agtagctcca ataagtgtaa
     4801 atttaggaag gtcaagtctt atgcttctag cagatggccc tttccctatt attatatcta
     4861 ggcaaaaatc ctccattgct ggatataaaa cttcttcaac acttctattg attctatgaa
     4921 tctcatctat aaataaaaca tcattttcat ttaaattcgt aagtatggct gccaaatctc
     4981 cagctctttc tattgcaggt cctgacgtaa ttcttaaatt tacccccatc tcattggcta
     5041 ttatacttgc tagtgtagtt tttccaagac ctggtggtcc atataaaagc acatggtcta
     5101 actgctcatt tctcgattta gctgcttcta taaatatact aagttgttct ttggattttt
     5161 cttgacccaa ataatcttct aaagtttttg gtcttaaact gttttctata tctatatctt
     5221 ccatcttcat tgtagaagtt attatcctat tttcatcttc aaaaccttgc atttataccc
     5281 ctcctctcgt acttttgaaa atctacttta tatattttca aatttacata aactccttaa
     5341 atattgtttt aaaatccaaa tattataaaa tccaaatact atttcaagga tttactcatt
     5401 atatatgtaa gtgctttctt tataatagct tctgtattca taccatcttt tttacatttt
     5461 tgaactgcct cttttgattc cgacaaagta tagcctaaag ctacaagtgc atctacagac
     5521 tcatcttgtg atatcaaggt aggtttttgt gataataacg ttggttcaaa ctctatgtta
     5581 tttttgtcta ccttatcctt aagctctaag acgattcttt ctgctgtctt tttaccaaca
     5641 ccaggagctt tggataattt tccaatgtct tcacttaaaa tatatgctcc taactgtgct
     5701 ggtgatgcaa atgatagtat tgaaagcgcc accttaggtc ctattttaga tacagatgtt
     5761 agtaattcaa acatcttaag ttcttcccta cttacaaaac cacaaagact catatcatct
     5821 tctcttacaa ttaatttagt atatattttt gtggcttctc ccacttgtaa attcattatt
     5881 gtattggatg atacatttat tttatatcct atcccattat tttcaacaac tatactgtct
     5941 atataaattt cttctactgt accttttata taactataca tacttaccac ctatattctt
     6001 tagagttttt tcaagtttgt ttgaatgagc atggcaaata gccacagcta aagcatctgc
     6061 aacatcatct ggttttggaa ctttttttag acttaaaaat gaagttacca tctgttgaac
     6121 ttgtgcctta tctgctctcc catatccaac tacaccttgt tttacttgaa gtggagtgta
     6181 ttcatataca ggcttcccat tatgagcaca tgcaagcata gtaacacctc tagcttgtgc
     6241 aactgttata gctgttttta cattcttgtt aaagaataat tcttccattc caacttcatc
     6301 tatattgtaa ttctttacta ttgtatcaat tcctttatac acaagttcca atcttctcga
     6361 tatattcata tgggcaggtg ttgtaactgc tccataatcg attgctttaa acttgctatt
     6421 tttgtattca attataccat atccaactat ggctatacct gggtcaatcc ccaatattat
     6481 cattttttaa aattccccct aaaaataaaa gaaataccaa aatgtatttc tcataaaaac
     6541 ttatgttcta aatcattata tcataaataa tattaacaaa tcataaaatg ctctatgtaa
     6601 aatataaaat caaataaaaa agttatatca aagcaagatt ttacctcaat ataactttta
     6661 tatttattac aaataaatta ttgtattttt ataggaatta aatgtgacta caattttaat
     6721 actaatctat ttaatattaa attatttatt attaacttac ttagtattta acttgaaata
     6781 atattaattt actattaact tgcaaagtct tctaatcttg aataggcctc ttcttcagta
     6841 tccataacta cacctttttt gatttcatct tgaactgttt taggaagtga atctactctt
     6901 atttttgttt tttctactaa tgttttactt ccgctatcat catatttata tagtgctata
     6961 tttccattat cattttcaat agtatattta ccttttatag taacatccat tgcttgtaca
     7021 tctttattat cactatattt agattctacc aatatgattt catttttatc catactcttt
     7081 atttttttag ttggatattc ttttttaaaa tatgatacta tttctgactt cgtcttatct
     7141 aataatgact ctggaactat tcctgccatt gtaagtgttc tcttatagtt agtttcattt
     7201 tcgccttcac tttgaagcca aacttcacaa tttttattta atccaaaagc ttcttttgac
     7261 ttttcattca tattcgcact agtacttaca acttcaccag tttgaactcc attgtgtttt
     7321 aatcttgtcc ctatataaac tccactactt aatacaaata ctccaatcag tattaaggca
     7381 gatattttcc aaggaaataa ttcttttttc ttcttttcaa acattgatta cgcctccttt
     7441 ttctattatt ttctccaagc tgtaagtttt tatacaattt aggtaaattt tagaagaaaa
     7501 tatatttata tttcaaatta ctctaagaat agatattttc taaaaggtag ttttctctta
     7561 atggttatat tcttgtaatt atatggacat aatttataat aaataatcgt ttaggaggta
     7621 ataactttgg aaagaataaa ctgtaatgta attaactgtt cacataacaa cagccatata
     7681 tgttatgcag ataaaatttt tataaatggt gaaaaagctc acaatgacag acatacttgt
     7741 tgtagttcat ttttagatga atctatttat agtaatttat ctagcaacgt taataataga
     7801 aattgtgagg aattagtttg tactgtaaaa aattgtaaat acaacaaaga tggtagctta
     7861 tgtacacttg ataatatatt ggttgctcct gaaactgata gagtaaatct ttatattgaa
     7921 acatgttgct ctagcttcga atgtaaatag tcatagtata aatataaggg tgtcaaatgt
     7981 aattaaacta acatagtgta gagtatctac aactattagt ttgattatac ttgacaccct
     8041 tatttatttt aaataaaatt tcattattat aaccatgttt ataatgttct aactgtaatt
     8101 aaatttgata aatttctata aattggtata atcaatatgt aaattgaatt tagtaaatta
     8161 atcataaaaa taaaatatcg agtatatagt gttatactaa ctagaaatga ggtgcttatg
     8221 agtacatttt atattcaatt tctttatttt gtaatttata gctttatagg atggtgttgt
     8281 gaaactacat attgttcagt tttacaaaaa caatttgtaa atagaggttt tttaaatggt
     8341 ccattttgcc ctatatatgg atttggagca ttagcaatag ttgctacctt gactcctttt
     8401 gtacacaata ttcctttgtt atttttgttt agtataatta taactagcat aatggagtac
     8461 tgtacgagct ttattttaga aaaaatattt aatatgactt ggtgggatta ttctaaacat
     8521 aaatttaata ttcatggaag agtttgtctg gaaaactcac ttatgtttgg tgtattatca
     8581 ttgattgtaa tgcttatagt acatcctatt gtggttgact ttattaactc tatctctaaa
     8641 aatgtgctat ttatatttgc tatatctata gaaatttatt ttgttttaga tttagttatc
     8701 acagtccata caattttaca attaaatggt aagttaaagc aaatcaatct tataattaag
     8761 gaattgaaag ataagaaaga atactacaaa cttattacac aagaaactat agaaaacaaa
     8821 cttgaatctt tagttgaaaa tagactggat actttggaag ataggtttga aatacttaag
     8881 gaaaaagaaa actatcgcta tgctaaaaat cgtattgatg agctaaaaaa taaacttaat
     8941 actttgctgt ctaaccacaa attacttcat agacgtataa tcaaagcatt ccccaacata
     9001 agctctaata agcatacaga tattttaaat aaaatcaaag aaaatattaa atcaattaaa
     9061 aaagataaat aataaaataa acacataagt taaataataa aaatactaat acataagatt
     9121 aaggtttaga atattttttg taaaatactc taaaccctaa ttaatatacc attattttta
     9181 atcttaattt ataattttag tttattttac ttttaacaca gtaaatttaa gcgtttaaac
     9241 ttcaatacac taatatacta ccccatcaat atctcaactg attctctaag tgtgctaatc
     9301 tctcccacat ttccagggaa aattatatat ggcatattag gaaatttgct ttctttgcct
     9361 gtcatccata ctggtatacc aggttttatc tgtcccatta ctgtagcttt ttttactctt
     9421 agtgctttag taccaacatc actagaagta attccaccct tagctataat aaaatttggt
     9481 cttacattaa gcataccaat tatactggta actgcatctg atatttctac agaaatcata
     9541 agctgtttat ctttatcatt cgtatccaag tcaaaacgct ctcgtctagt ataaacagca
     9601 acagttttac cactgcttat tttttcctct acaactttta taactctttt tacctcattt
     9661 tttagaccat tttcttgaag aactaaatgt tgattaaatt ctataaattc tatcggatat
     9721 ttacaatttt taagttcttc taactgttgg gtagtcttat tcacatgtga accaacaagt
     9781 ataattcctc cattggtatt accttttgat actaattcat ccttagtaag tagttccttg
     9841 tcactcactc cgccaagtac ttttgttatg gcagcagcgc ttctaaacat aaattcctta
     9901 ccaccattta ctgctcgtat aaacgcaatt gtaaatacct ttacatctac ataatcggtt
     9961 gcatttacaa taattttatt gaatccttca gcttttttaa gtttttctgt tatagcttca
    10021 atatttagct ctctaagttc ttttgacgaa atatatatca tatcactaga tttgtacttt
    10081 ccatttgtct tttcttcaca ccactcacct atatcagatg atttatatcc aaaagattta
    10141 tcttttgcaa attcagtcat tcctgctggt acaagtattt ctccttcttt aacataatgt
    10201 acattatcta tagtaaacct accaccttct ttaaaaaatg gcattattat ttcaccctga
    10261 aataacttcc ccgataatct ctctacttcg ctttttaata aattagtttc aagaggataa
    10321 tgtcctctca tagtagaatc actacgactt atgattataa aatccttatt aactttcttt
    10381 gatacatcca caatattctt tgaaatttct ttatgtgctt ttgtagtttg agcaacagta
    10441 aatccccttg aattggtaag tataaaaaac atactatttt tctcattaaa gccttgttcc
    10501 atactatcct tatcccaatc agtataaaca gaaatatcat gaactgtttg tacacctgtt
    10561 gggtcatcat ctaatacaac aatttttcta tctaaatgct taagctcatc tgcaagaagt
    10621 ttatttaatc tatcctcatc tacttgttta aaagtattaa gtatatctat gctaattggt
    10681 tttacttctc tcatttaaaa cgccccttta tgtaatttgt atagtctaaa tataatttat
    10741 atgatataaa caatagtttg ttatttatag acctggaagt ggtataattc catttataac
    10801 actaagcaat aaaaccatta taaatgctac aactccagca atggttcctc caattgtcca
    10861 agagtttaaa gtatctttaa ctgataatcc actaaatctt gaaagtaccc agaatccaga
    10921 atcatttgga agggagaaac caactcctcc agcacatata gcaagcccta ctaaaactgg
    10981 tgacattcca gttgccaaaa ttgtaggtcc taaaattgat gatgttgtta ctaaggctac
    11041 agtagcagaa ccttgagaaa ttctaagcaa ggcacttaat aagaatccaa gtactacaac
    11101 tgatatacta agaccagaca tagtatcaac taagaagtta cctataccac ttgcatttat
    11161 aacactgcca aacgcaccac ctgaccctgt tatcaataat attagccctg ctgaatcagc
    11221 agcttcaata actacatctc ccataggttt atttatgtat ttctttaaaa atcctattgc
    11281 caccaatatt ccaagaagta ttgctatgtt tttatctcct ataaatgcaa gtactccact
    11341 cattgtagaa cctttttcta agaacatact aaatacattt gatataagta taagaagtat
    11401 tgggaacaat aaagaaaaca tagaaattcc aaaacctggt actttagaag aatcatcatc
    11461 ttcaattttt atgctatctt ttccctgctc taaatttttt tctctatcat tctcatcaat
    11521 agcataagct ggatattttt caaaacgttt tccataaatc catccaccta taagagctgc
    11581 tggaaaagca acaattatag aataaagtat aaaactacct acatttgcat ttacatttgc
    11641 tgcaactgcc aaaggtcctg gtgtaggtat tacaagtgca tgacctacta tagtcccaac
    11701 tcctaaggca catacaaaaa ccattagaga tttcttagtt actctcgaaa catacttaat
    11761 tataggcatc attataacaa aagctgcatc cataaaaact ggaattgaaa ttaaaaaacc
    11821 tgctgtagtt actgctaaag cactgttttt ttctccagtc ttagctaaca acccctttgc
    11881 aatagattct gttgctcttg attctgaaag aatatttcca aatattattc caagtccaat
    11941 tacaatacct atactaccta atgtatttcc aaatccagtc gatattgtgg ttgatatttc
    12001 atttataggc attctaacca taaagcctgt aattatagat gttatcagta atgccaaaaa
    12061 cggatttact ttaaatttaa taatcgatac aagtaaaatt cctatagata taacaaatgc
    12121 tatcaaaagt ccatatcctg taatcataat ctttacccct ttatcattct actttattat
    12181 ttatcaattt tatttttctt ttactatgat aacttaacct cgctgtattt atataatcta
    12241 atcctaataa tatgtattta attatattga tatatttaaa tattaattat cgtgtttttt
    12301 tatttcaaca cctgccaact tttcaaaata ctgtgctatt ccactatggt catcttccaa
    12361 atgtccatgt acttttaatg tctgcataat ttctaataat tgacttgtca taggtagtgg
    12421 tacatctaaa ttgtgtgctg tttgcattac attcttaata tctttcaaat taatagatat
    12481 ttttccacct ggtttaaaat cacgattcat aatcattggt atcttcgcat ccaatattgt
    12541 actaccagct aggcctcctc ttattgcctt atatactttt tctgggtctg cacctgcttt
    12601 tgctgcaagt acaaatgcct cagaaactgc tgctatcgtc aaattaacta ttacttggtt
    12661 tgtaagtttt gtaacacttc cactaccatt atctccaatt aaaatagatg agcttcccat
    12721 tatattaaaa aatggctcta ctttcttata aatttcttcc ttaccaccag ccataaaagc
    12781 aagtgtacca tcaatcgctt ttggctcccc tccacttact ggtgagtcta taaattctaa
    12841 gcccatatct tttaatttat ttgcacacaa tatggattct gtaggagtaa ctgaactcat
    12901 atcgactact atacttcctt cttttaaagt ttttgccaat ccatccatcc caaacaatat
    12961 atcctgcaca atagttccat ttggaaggat tgtaaataca atatcacatt cttgacccat
    13021 ttctaaaaca cttgcgtatt ttgctccaca tgatataagt tcatccacag cagatttatt
    13081 aatgtcatac accaaaagat tacatccatc ttttaataaa ttttttgcca ttggctttcc
    13141 cattattcct aatccaataa atcccaattt cattttataa ccctccatat cttcacattt
    13201 ttgaaaacgt tttttatgtt ttagagtata ctacatacca gtataccggt caatataaaa
    13261 tataaaagta tttattttaa caataaatag taatttttaa agtcatttat tatatttagt
    13321 catcctatat aagttttaat acatactatt gttttcaaat ataatatact acttttacag
    13381 ttttgtattt taactaattt caattagttt taattaatct gcctttaatg ttaatcatgc
    13441 ttcacacttc aataattttt tatatttttt aatcttatct gcaatttaaa ttgcaaaaga
    13501 ttgaccttat tttcactatg ttgtatgctt aaaattatat aagtttttgt atttttatat
    13561 tgtttagggg ggtattatta agtatggcta catttaaaag attacaagaa caagcatatg
    13621 actacttaaa agaattgata ctatctggaa aaatgataga aaatgaaata tattctgaaa
    13681 caaaacttgc tagtgaaata ggtatctcta gaacacctat aagagatgct cttcaaagac
    13741 ttagtcaaga tggatttata gatataatcc caagcaaagg atttagaatc catcaaatta
    13801 cagcaaatga aattgtagag atattccaaa tacgctctgc aatagaagga ttttgtactt
    13861 ttttaattac aagtcaatac aaagaagcta gagcagtaga aactataagt aaattaaaac
    13921 atcttttaga taaacaaaaa gatattttat taggtgataa aaatttaaac tcatttgcag
    13981 aatacgatac attatttcac acaactatag tttcatatgc tcaaaatact gaatttgaca
    14041 aaatgtttaa caattatatg tacagaataa aaaaattagc tctcgattca ttgtcacatg
    14101 aaggaaggct tgaaacaaca ttaaaagagc acacagatat ttttaataat attgcaaatg
    14161 gatgtattga agatatttac agaactactc tcattcatat ggaaactcct aaatacatta
    14221 atttagaaga tttttgtaac aataagttat tttagcatta tactttcatt tattaaaaat
    14281 atatctatct caaattaatt tcctaattta taaaaaatat tctataatta tattgtaact
    14341 aatacttatt ctaaggagtg atttttttga acgaggatgt tgtaactaat gaaactatcg
    14401 acaaagatta tgctattgaa gaagttcgta tggcatgcaa acattttggt gatttatatt
    14461 tttatttttc caaagtgtta tttgaagagt ttggtgaaga taaaactagc gaaatattaa
    14521 gaaaagtttt atttgaaagg tctgaagaaa gagctattgc tatgagagaa agagctcatg
    14581 aaaatggaga tgaattaatc gcagacaata ttattagcac aactgatgtt ccatttctag
    14641 gatgggttcc tgaatttgaa gagttacatt gcccctatgg agcttcttgg cttttaagat
    14701 ttgaggaaaa tccttggttt aaaaaatttg cttctcttta ctgtgatgtt acagatacta
    14761 ctgtagctga agtatttaca ggagatacgt cccataaaat aacaaaaaac attctatggg
    14821 gagataaaag ttgtgaaaga atatatttcc atgatgacaa ggtatctgaa ggtaaatata
    14881 cttatggaaa taaaaactgt aaataaataa tttaaatttt ctatattcat atactatatt
    14941 ttgtactttt tcatataaat attataacta gtcttttatt ttacttttgt ttaacctaaa
    15001 gataatttac ttttaatcta tatgaattat catataaata atcagaaagt tatctttata
    15061 aattatagtt ttagatttat aatttataaa atatttatgt tctttgtaaa attttttaga
    15121 agcttctaat gttatgctat aaaactatct tgagaagggc tgattgaatg tttaataaaa
    15181 tatatgaaaa gaaagaattt atagtttttc aagttaaaaa aggttatgta gtttataata
    15241 ctagaaagag ttttgaagaa ggtcatacac atttaaagca ttttgaagca gcaaagacag
    15301 ctattgattt agcaataaat aaaaagattc ctagaagcaa agatggatat tatctgacta
    15361 gtttaattcg cataagtgat gatggttatt acattgataa gttaagcgaa ttgttatatg
    15421 ttagagaaca aaaaggtaaa aaagaaaagt actgtaattc tggttatcag atgtaacaag
    15481 actagttgtg aattataagt accaatcata agtaccttta gtcttaaaat caaagagtat
    15541 cagtaaatct ataaaaaaac aacttactga tactcttttt aattatatga ttaatatttg
    15601 aatttgatac aaaattttat acaaaagttt atgttatcta ttctgattct ttgtctgcaa
    15661 attggctatt ataaagcttc tcataaaatc cacccttttc cattaattca tcatggttcc
    15721 cctgctctat aatacttcca ttattcataa ctaaaatcaa gtcagcattg cgaattgtag
    15781 aaagtctatg tgctataaca aaactagttc taccattcat aagatttctc attgcttttt
    15841 gaagcataag ttccagccta gtatcaacag aactcgtagc ttcatcaagt atcaatattg
    15901 aagggtctga tataaaagct ctagctatgg ttaaaagctg tttttcccct agagatatat
    15961 tagatgcttc ttcatttaaa aacatattgt atccatctgg aagagtggtt ataaagtgat
    16021 gaacatttgc tacttttgct gcttgtataa tttcttcttt agtagcccca aatctaccat
    16081 attctatatt ttcaaaaatg gttccattaa ataaccatgt atcttgaagc accataccaa
    16141 acattgaacg caaatcttta cgtttcatat ctcttatatc tactccatca attttaatag
    16201 cacctttctt tacatcataa aacctcatca ataagtttat taatgttgtt ttacctgctc
    16261 cagtaggtcc aacaatagct accatctgac cactttttac ttctgcattt aaatcttcta
    16321 ttaaagttga attttctcca tatccaaaat taacatgctc aaatgtaaca tttcctttta
    16381 cattttctat cttaactgga ttttcaacat caggtatttc ctcttcttcg tctaaaattt
    16441 caaatacacg ttctatagct gcaaaagaag attgtattac tgtagataac tgagtcattt
    16501 gtgaaagtgg ctgatttatc tgccatatat aacgcacaaa tgcttgtaaa tttcctactg
    16561 ttattcctcc acttactgca tatatagaac cgacaactgc cacagctgca attccaaagt
    16621 aagttactaa tgaaaccata ggattcatca tacttgatat aaattgtgcc ttaaatccat
    16681 tttcacaaag ttcttgattt acttttttaa attcttttat agaatcttct tctttaccat
    16741 atagttttat ttcattaaat ccagtataca tttcttgtac tttcccattt aattttccaa
    16801 gagcattttg ttgcaatgaa aatctacttt gagatttttt tactacaaat ttagatacaa
    16861 aataactaat tggaattata aaaagagcta taagagccat atatatatta attgtaaaca
    16921 tcattgaaag tgccaaaatt atgactaaga ttgagtttac aatttgcata aaactctgtt
    16981 gcaatgcatt tgatattgta tctacatcat ttgatatacg acttaataca tctccaaaac
    17041 tattactatc aaaataacta ataggcaatc ttcttatttt attttcgact tcatttctta
    17101 aatctctcat tgtgttttga atagcattcg taagtagaaa acttgaagta tacgtaaaaa
    17161 ttgcattggc aaaatatata catgctaaaa tcaccaatat ttttgttaca taatcaaaat
    17221 ttacggaagc ccctggtact ccttttgcga tactaattac atctttagtc aattgtgtta
    17281 ttatcaatcc ttcagtttta ggtgcaagtg cattcatagt agctgctgaa attatacata
    17341 taatagctat aataaaggaa aatttatatt tctttacaaa tggaagaagt ctaccaattg
    17401 ttttcttaaa acctttagac tttttcatta ttctaattcc tcctttttta actgagatgt
    17461 tgcaatttca tagtatattt cacagttttt taataattct ttatgagttc ccattcctac
    17521 aacttgtcct tcattgagta ctataatctt atcagcatca ataatagaac taattctctg
    17581 ggcaacaatc aatacaattg catcttttgt ttccttcttc aatttagaac gaagtattgc
    17641 atcagtttta aagtcaagtg cagaaaaact atcatcaaaa atgtatattt caggtcttct
    17701 tataatagcc cttgcaatag aaagtctttg tttttgacct cctgatacat ttgctccacc
    17761 ttcactaatc aattcatcaa actttctagg tttttttgat ataaactcat atgcttgtgc
    17821 aacttttgcg gaatattcta attctgactc tcctgcctta tgtttcccaa atctaatatt
    17881 atttgctatg ctaccagtaa aaagcagtga tttttgaggt atgaatccta tcttttttct
    17941 taaagctttt aaatcatatt ctcttatatc tactccattt atcttaatgc taccttctgt
    18001 tacatcataa aatctaggta tcaagttaat tagagtactt ttcccactac cagtacttcc
    18061 tataaaagcg actgtttcac ctgtttttgc agtaaatgaa atatccttaa gtacagaagc
    18121 ttctccatct ggataggtaa aagttacatt atcaaattct atgataccct tattttcagt
    18181 atccttaatt ccattttcag gattttttat caatggttct tcatttaaaa gttcttttat
    18241 tctatttgca gatacctctg ctcttggata cataataaat accattgaaa aaagcataat
    18301 tgaaaacatg gcatggaaaa gatattccaa aaatgcaacc aactgtccaa cttgaagttc
    18361 tccaatattt atcttctcac ttgaaatcca aaatacagcc aaaacagcta tgtttaatag
    18421 taaaaagaat gctggttgag taattgacat aagcttaaat aatttcttag aaacattagc
    18481 ataatatgta tttgtttttt caaatcgctc ggtttcataa tcatcatttc caaaagctct
    18541 aattactctt attccagtta aattttctct cgatataagg tttaatttat ctagtccttt
    18601 ttgttgtttt gaagatattg cctgagatac ttttgctatt attgctactc caataattat
    18661 aaaaggtact gatatagcca atactataga aagttccaca cttgttctaa tggtcattat
    18721 tatacttata atgaacataa ctggagttaa taaggcagtc cttagtagta tattaacaaa
    18781 ttgttgaatt tgaaatgcat cattcgttgt tcttgtaatc attgaagaaa ttccaaattt
    18841 attgtattca gtatgtgaaa attcttgtga tttctcaaat atgtcatttc ttatatctct
    18901 ggttacactt gttgatacct tagcagaaca ataacccaac aatatactac ctacaacccc
    18961 tataatagaa ataataacaa tcactattcc catttgtttt atgtaagcta tattctgatt
    19021 tgcaatacca ttgtcaatta ttttagctat tatagttgga atccccaact cgactaatgc
    19081 aaaactaaat atagccagta tgtttaatac tattaataac ttataattct ttaaataact
    19141 taaaattaat ttcatatttt tctcctttct taatttttga gatagtgtaa tcatgtaata
    19201 atactaaatt tataaatgct taattttata aaaaaaggag aaaagagtta acgcactctc
    19261 ttctccagca tattcttttt aatacaccct ccgacaagtc gcttatatgt taacatattt
    19321 gacaagtaat gtcaattcat atagccaaaa gtcttttaaa tgcttcgttt cataacattg
    19381 taacaaattg gcatgtaatt tatttttgtt ttttactgta ttccaatttt acaagttctc
    19441 caatattact agctttctta aaattcaaat tagtcttatc tataccagac ttaaatagtg
    19501 aaatccctct ccccaatatt attggcaata tatatatata ataatcatca attaagttat
    19561 ctttcataaa taattttact atatcgcctc caccaactat ccaaatatct tttttatcct
    19621 tctcttttcc aatttgctca attaattttt taggttctaa attaatttat agtgtgatta
    19681 ttctatgtaa acatcacttt tttactcatt caataatacc ctattaataa atcctcaaac
    19741 taatttaaat aaactttgca taaaattaaa aatatattat ataaataatt taaaactaca
    19801 ctaacttatt gaaaattcta attattacaa ataaatattg ctctctgtta acgctagttc
    19861 tataatataa ttataagcta ttaatattat aaagccgata ttactaatat aataattata
    19921 atacttatag ttaatttact tttgttgaaa ggtggtaaat aacatgaaaa tttcagaagt
    19981 cgctactcca aattttttgc tagatttaga ccagttagaa aagaatatag ataaaattca
    20041 acaaatttgt acaaataata ataaacaact ttggcctatg cttaagacac ataaaagtac
    20101 ttacatagcg agacttcaaa aaaatgcagg tgctagtggt tttctagttg gaacacttga
    20161 tgaaggtgaa gctctgataa aagcaggttt tgaagatatt atgttggctt atccttacat
    20221 gggagaagta aatataaata gaatcgtgac tatggcatca aaaataaatt taatctgtgc
    20281 tacagataat atagaatgtg ctaaggctta tagtgaagct ttttctaaat caaaaataga
    20341 gtgcaagcta cttattattg tagattgtgg cttacaccgt tttggggttg aaccagaaaa
    20401 agtggttgaa cttgctactg aaatttctaa gctagaaaac atcaagattg ttggtataag
    20461 ttcgcatcca ggtcaagtgt atggttgtag ttctccagaa ggtgttccag atgtttgtgt
    20521 tcaagaagat tcttctatgg agaaagcata caaagctcta actgataatg gatttaatat
    20581 agaaattgtc gcttctggta gtactccaac agttgaatct gaagctgctt ctaaaactat
    20641 aactgctgta agacctggaa actatgtata ctatgatgct atacagatag gtttaggttg
    20701 tgctactgaa gatatgtgtg ctcttactgt tgtgtgcact gtaatctcaa aaaatagcag
    20761 tggatactat ttaattgatt gtggaagtaa gtgccttgga cttgataaag gtgcacatgg
    20821 aaatagtagt attgttggtt atggtagggt atgtggacat ccagaactta ctattgattc
    20881 actttcagaa gaagttggaa aaattaaagt aaatggtgaa actgatataa aaattggtga
    20941 tagaataaga attataccta atcattcttg ttccactgca aacttgacaa gtaatttatt
    21001 aggttttaga ggagatacaa tagaaaaagt catagaagta gatattcgtg aaaattcaaa
    21061 acaaatcatc ctttaaaaac acatttattc tgatattaac atgaaagtat atttttctta
    21121 aaattattgt taataactaa tgttaatgtt ggataaataa aaaacataaa ttctagaaat
    21181 ataaatttaa aagaagaggc tgtctcaaaa taaagataag cctctctttc attgttgaaa
    21241 ttatgaaaat atacttgatt tcatacaaaa agagtatctc atgaactaaa aagttcactt
    21301 tgagatactc tcttttttta attaatatac acattaatta ctatcttagt ttattactat
    21361 atatcaatta ctactcatta atccttgtat atattaattc ttatatatca tttatagtta
    21421 ggttataatt ttatgttatt tacattaatc ccataacagt gagtaaaact cttactatta
    21481 acatccccac tattgcatca aatataggaa ctgccaacag taacattcta tacttagtac
    21541 tggcatttgc tacaagcact attctggcat aatgaccaac tagagtaccc attaacatac
    21601 atggcataat taatatggtt gcttgtggtg cagtgattag accttgtgca aataagtttg
    21661 cagctgtagc tgctcctgct gccttagcaa agaatgctgc tatcaaaact acaacagatt
    21721 ccccaggtag tccaaaaata cccattactg gtccaaaaat atttcctaaa atatcaacta
    21781 aaccagttaa ttgtaaaaac tgcactatta cataacccat tatcatggct ggtaatatct
    21841 gttcaacacc tatgtaaaaa cctttttttg ctccccccat aaatatctca acagaagttt
    21901 tcttaggttt actatttaca attgcatttc cagtattttg ttgactcata ctacacagcc
    21961 tccttagcct tattcttttt atttcttaca cctatgatta atctaactag attagcacca
    22021 agtattttac agaatatctc tataaggatt attggaccta atgccaataa tgctattggt
    22081 aaaagtggag cttgagtatt tattgtgttc agtataacag ctgaaccagc atattgatat
    22141 gctacaaata tagttctttc atcatctgtt atttccccac tttcaaataa ttctttcgtc
    22201 attattgagg ctacatctga actagtaaat gaagatacaa atgctattcc tgctattcca
    22261 ggtataccaa gcagaggtct aagcaatgga ttaaatatct ttgatgccgc tttcattcct
    22321 cccatagatt caactacatc tataagacct acagcaaaac agacagttgg aatcaaagtc
    22381 agagcaaata agaaaccatc ttttgctccc gttcctcctt tacccataaa gtctacacca
    22441 tctgcaatag taccaaatga acctgtcaga ttagcaaaat ctaatgcttt aaggaagcct
    22501 tgactatctt taaacacccc tgaaaataat actatcaata caaataaaga taaataccct
    22561 ttccatgtta ccttattatc tccagctgtt acatccatat aaaatccccc tttatacctc
    22621 aaatttatct aagtttaaaa taactgtaga aattaatttc accctctcaa acatagaatc
    22681 tactactgca tattctcttg atgtgtggtt ccattctcct cttacaccaa atgaacagat
    22741 tgtgggaacg tttgcaatag ttaaataact tgcatctgaa ctacctccaa gtcttttagc
    22801 atttaattct ccaaatccat tttcttttga cacttcattt acaaatgtat gaaatctcat
    22861 aacatcttct gtagtttcaa atggcatcat ttcacttata aaattaactt ctgtagaagt
    22921 tccctctatg taagtttctt tacatatctc tctaacttgt tctttaacat tctccatttc
    22981 ttctactttt tcaaatctaa ggtctacttt tatactacaa tcttctggaa tagaattttc
    23041 cactcttcca cccttaatca cacttacact tactgtagtt ccaacatcta aattagtcaa
    23101 tttttgtatg cgtagtatct tattagccat ttcttctata gcatttcttc caccttcaaa
    23161 atcattacct gcgtgtgttt ctacaccttt tacatgtata ttacatccta ttcttccttt
    23221 tctacctaca cacaaactat tatctactaa tccagtttcc atattaaaag cacaaagacc
    23281 accttttgct tccctcaata taacatctgc acctgttgaa tccttatgtc ctatctcttc
    23341 atctccagaa aatactattt ttattggtct ttctttgtat cctattttat ttaatgcttt
    23401 aataacatat aaggaaatta ttataccacc cttcatatct aaaacacctg ggccatatgc
    23461 ttttccttct attattttaa atggattctc tccaaatgta cctgtttcaa agacagtatc
    23521 catgtgacct gaaaaaatta taggcttttt atttatatta gaaccaagtg tacctatcaa
    23581 tgtactacca ttatctccaa catctactaa ttcgcaatct aaaccttctt tttcaaattc
    23641 aagctttaat ctctcagcta acttgtttac actttcttta caatgtgtat agctttccat
    23701 attaacaatc tctttccata atgaaactat ttcttctcta ttctcatcaa caaatttaga
    23761 aatttcattt aaatatgtat tcataatctt ctcctttatt ttcaaataaa atgtatttct
    23821 ttcctaaaat ctaagtttat tttaggaaat cttaaaagac tagtccttaa ttacaaaata
    23881 gcacaattat tcttgccagt caacatattt ttcagtcttt tttatatttt ctgatttttc
    23941 tttcaatttt ccttttgtaa aataaattga cgcgttttat ctactattat aaaatgaggt
    24001 tataaattga ttgattagtt acaatactct cgaaatttta aaggaggttt tatgaaatat
    24061 aaaattggtt ttttgacaac atcttatttt atgtcagata agtttaaaca agctcttaaa
    24121 gaattaaatg atacttgtga aattacattt attgagctca aaggggagaa tccttttaat
    24181 aatttaccta atatatacta tgagcaagta gataattttg atggatttgt atgcagtggt
    24241 atagttccat atagtgaact tataataaat ataaaagaca taaaagttcc attaaatttt
    24301 ttaaagcttg atgaaagaga tttttataaa tacttattca aattattaaa tagccaaaaa
    24361 gatattgact ttacaaaatc atttatggat tttttaagag aggacaacaa ttattatgat
    24421 atatattcat taatagactc taataaatgt ccatatacaa ttaaagattt taatatccct
    24481 caacctgtat atgactttaa aaaattaaat gataaattat tggaaataca tttagactta
    24541 ttgcgagata ataagattaa cctttctttt actagaaatt acatcattag ttgtgaactt
    24601 aataagcttg gatatgattg tatttacatg gttccatcta cagaatcaat cctgaataca
    24661 tttaaatctc tggtgaatga aataacatta caaaacttag acaaaaataa atctgcaact
    24721 tgtatagtta cagtaaatag ttcagagtac ataaatgaag atttgatatt taaaaatgat
    24781 gagatacaaa atcaaatata taacactata ttaaattctc tatcaagaca tggattctat
    24841 ggagttcagg tcaaaaaaaa tgatatgaaa atagagatac acactactaa agaaatgtta
    24901 aacaatatga ccaataacta tacagatttt tttatttcag aagatttaaa agaaattaaa
    24961 tatagcttaa atataggttg gggcattgga aatacaaata ttcatgcaga gcaaaatgct
    25021 cgtcaagcta atggtaaatc tgcttctttg aatggaaact gtacttttat agtaaatgat
    25081 actaatgata caataggtcc tttatattca aataaaaatt ctaataatat tgaagatagc
    25141 tcaatagcca ataaagttgc ttcttttata cctctatcta atacaaatgt atctaaaatc
    25201 atgtgtatga ttaatgatag aaattcaaat aatgtgtcag cagaaatatt agcagactat
    25261 atgaacataa ctcttagaag tgctaataga atattgagta ttttatataa agcaggaatt
    25321 gcaactattg taaatacaaa actcgataat caaagaggaa ggccaaaaaa aatatataaa
    25381 gttgatttct tatcttttct aaataagatg cagaaattaa attcttaata tttaattttt
    25441 taataaaagc aatgtatttt ctctaatttt aaggaatcaa aatgtttaaa ttttattaga
    25501 atatacaact ttttacttta aaatacctaa accttttatc agtgaaataa accataatat
    25561 atcgtaactt attagaaaaa caaaataatt tcatatacaa ataataaaat agccactaca
    25621 atccataatt tgcagtggct actttttaca ctatcactta ttacttattt ttaaaaatta
    25681 attgtatttt ttaaataatt tatacacacc tattatcagt aaagcaataa atacaagtat
    25741 atctataaaa ataattattg taacatgtct ggcaatattg taaataaaaa gtatagagaa
    25801 tatttctata gacaacgcta caaaacacat aaaattcgtt aaataatctg caatagtatc
    25861 actataaaaa gcatgtattt gtaaccatct ccatataaat ggacgtaaaa gtattctgaa
    25921 tacaacacaa atgacagggt aggcataaat aaatttttta tctacataat tagctacacc
    25981 attattccat tggataggaa tttgaggtgg caactttgga aatactgtta ttgcaatcaa
    26041 caaacaagca atagtaataa ttccccaggt aattttccct ccccatatat ataagaaact
    26101 tacagagtca agtttaaaaa caggtttgtt tttattccag tattctggca actcagttat
    26161 gtattgctct actttcaaat tcaaataact tagctcatca gattctagca ttttattaca
    26221 catagttttt gcattttcta agtctgatat tttagtttcg agaactttct cctgtttctc
    26281 tacaacttca actaaagata ctttttgtaa tataatatta cggatgtcat caatagtaat
    26341 tcctaatgta cgtaaatatg caattttttt gatgttctct acattttcat ctgaatattc
    26401 tctatagcca ttacgctcat tacgaagggg tttaattaaa ttctcgttct cataaaaacg
    26461 aatattagag cgtgtcaatc ctgttttttc ttcaacttct ttaattgtca ttttaaacac
    26521 tacctctcta ttctattaat ttataactat aatatagtct taaatatttt cactctataa
    26581 aattgtatat ttagtatatc ttaaaatcaa actaa
//
LOCUS       DAMJYC010000051        26540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJYC010000051 DAMJYC010000000
VERSION     DAMJYC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.052"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(113..307)
                     /locus_tag="PC472_002308"
     CDS             complement(113..307)
                     /locus_tag="PC472_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727627.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(613..2142)
                     /gene="lysS"
                     /locus_tag="PC472_002309"
     CDS             complement(613..2142)
                     /gene="lysS"
                     /locus_tag="PC472_002309"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2727628.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2157..2636)
                     /gene="greA"
                     /locus_tag="PC472_002310"
     CDS             complement(2157..2636)
                     /gene="greA"
                     /locus_tag="PC472_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2727629.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2745..3713)
                     /gene="dusB"
                     /locus_tag="PC472_002311"
     CDS             complement(2745..3713)
                     /gene="dusB"
                     /locus_tag="PC472_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2727630.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3850..4620)
                     /locus_tag="PC472_002312"
     CDS             complement(3850..4620)
                     /locus_tag="PC472_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2727631.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4639..5229)
                     /locus_tag="PC472_002313"
     CDS             complement(4639..5229)
                     /locus_tag="PC472_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2727632.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5413..6381)
                     /locus_tag="PC472_002314"
     CDS             complement(5413..6381)
                     /locus_tag="PC472_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2727633.1"
                     /translation="MYNNILDIEGLKVGQIEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6374..7351)
                     /locus_tag="PC472_002315"
     CDS             complement(6374..7351)
                     /locus_tag="PC472_002315"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2727634.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTDFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWNSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7666..9636)
                     /gene="ftsH"
                     /locus_tag="PC472_002316"
     CDS             complement(7666..9636)
                     /gene="ftsH"
                     /locus_tag="PC472_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2727635.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9670..11058)
                     /gene="tilS"
                     /locus_tag="PC472_002317"
     CDS             complement(9670..11058)
                     /gene="tilS"
                     /locus_tag="PC472_002317"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2727636.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11241..11669)
                     /locus_tag="PC472_002318"
     CDS             complement(11241..11669)
                     /locus_tag="PC472_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2727637.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11686..12492)
                     /locus_tag="PC472_002319"
     CDS             complement(11686..12492)
                     /locus_tag="PC472_002319"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2727638.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12694..13179)
                     /locus_tag="PC472_002320"
     CDS             complement(12694..13179)
                     /locus_tag="PC472_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2727639.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13230..13889)
                     /gene="spoIIR"
                     /locus_tag="PC472_002321"
     CDS             complement(13230..13889)
                     /gene="spoIIR"
                     /locus_tag="PC472_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2727640.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14034..14714)
                     /locus_tag="PC472_002322"
     CDS             complement(14034..14714)
                     /locus_tag="PC472_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2727641.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14698..15588)
                     /locus_tag="PC472_002323"
     CDS             complement(14698..15588)
                     /locus_tag="PC472_002323"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2727642.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15706..17256)
                     /locus_tag="PC472_002324"
     CDS             complement(15706..17256)
                     /locus_tag="PC472_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2727643.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17350..17616)
                     /locus_tag="PC472_002325"
     CDS             complement(17350..17616)
                     /locus_tag="PC472_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2727644.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17764..18624)
                     /gene="yabG"
                     /locus_tag="PC472_002326"
     CDS             complement(17764..18624)
                     /gene="yabG"
                     /locus_tag="PC472_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2727645.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18708..19646)
                     /locus_tag="PC472_002327"
     CDS             complement(18708..19646)
                     /locus_tag="PC472_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2727646.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19756..19917)
                     /locus_tag="PC472_002328"
     CDS             complement(19756..19917)
                     /locus_tag="PC472_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727647.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19927..20424)
                     /locus_tag="PC472_002329"
     CDS             complement(19927..20424)
                     /locus_tag="PC472_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2727648.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKTYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20539..21249)
                     /locus_tag="PC472_002330"
     CDS             complement(20539..21249)
                     /locus_tag="PC472_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2727649.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21425..21955)
                     /locus_tag="PC472_002331"
     CDS             complement(21425..21955)
                     /locus_tag="PC472_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727650.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21972..22202)
                     /locus_tag="PC472_002332"
     CDS             complement(21972..22202)
                     /locus_tag="PC472_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727651.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22217..23611)
                     /locus_tag="PC472_002333"
     CDS             complement(22217..23611)
                     /locus_tag="PC472_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2727652.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23737..24990)
                     /locus_tag="PC472_002334"
     CDS             complement(23737..24990)
                     /locus_tag="PC472_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727653.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25041..26195)
                     /locus_tag="PC472_002335"
     CDS             complement(25041..26195)
                     /locus_tag="PC472_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2727654.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
BASE COUNT         8141 a         5124 c         2621 g        10654 t
ORIGIN      
        1 tgttttttat tttaaaaaag gttataactt ttggtttttt ttaataaata taattaaaac
       61 taatagatat aacccttgat attacaaaca atgcatgcat atataatttt acctattttg
      121 caattttatt ttcataagaa tatgaattag ctctcactct atctgcccaa acatttatac
      181 tcttaacttt attaacatta gttgtaccct caaaaatatc cattgcatcc ttacaaaaat
      241 atatcataac atcaagaacc aaaaatccaa cacaaaataa acataaaaaa ttaattactt
      301 gaaacatcag acattccccc aataattttt tatatatagt attctcataa attattcttt
      361 ttaccttctc ataaattatt ctaaatattt tcataataaa ttactttcaa tcatattttc
      421 taaatataaa gtctcaatca acaaaaacta atatcaattt ctaacgtttg ttggtaattt
      481 ataaattaaa tatctaaaag aatctttgaa gcatataaaa agcattctac taagttttaa
      541 ataaaattta gctatttatt gataatttaa tagctaaact ttatttaaaa cttagtagaa
      601 tgctaatatt tcttagtttt cttcttcttt atttggattg ttatcaatag gtttcatagt
      661 cgggaataat aatacatctc ttatagatgg agagttagtt aataacataa ttactctatc
      721 tatacctatt cctagtcctc ctgtaggagg tagacctacc tctaatgctt ttaagaaatc
      781 ttcatccatt tcaaaagctt catcatcacc taattctctt tttcttaatt ggtctataaa
      841 tcttcctttt tggtcaatag ggtcattaag ttcagaaaat gcattagcta actcccactt
      901 attagcaaat gcctcaaatc tatcagttct tcttgggtct tcaacatttc tcttagataa
      961 tggagatact tcaactggat gatgagttat aaatgttggt tggattaatt tatcttctcc
     1021 aaattcttca aagaaagcat ttattacttc tcctcttctc attccaggtt ttatctctat
     1081 acctttttct ctagcaactt ctagtgcctc ttcatctgta tttatagtag aaaaatctac
     1141 accagaatat tctttaacac aatcttccat actcattctt ttccaaggtg gtgtaaagtt
     1201 tatttctgtt ccttgatagt taactatcat acttcctgta gcaacttcag ccatatgtgc
     1261 aataacattt tctgttattt ccatcatatc agtatagtct gcatatgctt gatataattc
     1321 tattgcagta tattcaggat tatgttttaa atccatacct tcatttctaa acattcttcc
     1381 catttcataa actttatcaa atccaccaac aataagtctt tttaaataca attcattcgc
     1441 tattcttaaa tacattggta tatgtaaagt attatgatta gttataaatg gtctagcatt
     1501 tgccccacct gctatagtat ttaatattgg agtctcaact tctaaaaatc ctctatcatc
     1561 taagtatgct cttaatgctt ttaatgcttt tgttctaatt aaaaaagcat tttttacttc
     1621 tggatttact attaagtcaa catatctttg tctatatctt agttcttggt cttttaatcc
     1681 atgatatttt tctggtaaaa cttgtaaaga tttacatagt aatactacag atttagcctt
     1741 tacagatatt tcaccttttt tagttttaaa gatttctcct tcaattccaa ctatatctcc
     1801 aatatcatat gttgaaaata cactatattc ttcctctcct atagcatcta gtcttacata
     1861 agattggatt cttccctctt ggtcttgaat atctataaag ccagctttcc cttgtattct
     1921 cttgctcatt atacgtcctg ctatttttgt agttttaccc tcaagagaat caaagttatc
     1981 ttttatatcc atagaataat gagttctatc atatctactt tctttaaaag gatctctacc
     2041 tgactcttgt aattttttta gtttatctct tcttacttga agtacttcac taaggtcttc
     2101 ttgaatttgc gcttcttcat tgctttgttg attatttttc atttttttcc tccaaattat
     2161 cttcttattt ctaatatttc aaacttagca actccatcag gaacttgcac ttccacaact
     2221 tctttcgcag ctctacctaa aagtgctttc cctacaggag attcgtttga tattttacca
     2281 tcatatgggt ctgcttctgt cgaaccaact atagtatatt caacctcttc atcaaattct
     2341 aaatccttaa ctttaactat tgatcctatt gtaacaacat taaggtctat tttgctttca
     2401 tctatgataa cagcttttct aaccatattt tctaatttta aaattctttc ttcaacttgt
     2461 gcttgttcct ttttagcttc atcatactca gcattttcag ataaatcacc aaaagatata
     2521 gcaactttta ttctctctgc aacttctttt ctctttacaa cctttaagtt ttctaattct
     2581 tcttctaatt tatcatatcc ctcttgtgtc aataaaaact ctttattttc ttccattata
     2641 aatacaactc cttcacaaat ttcttaggaa ttttcctaat tatattttaa aaattttata
     2701 atcttttttt aatttatgcg ctatattata agtcactatt atttttatgt caaggagtct
     2761 tccatatagt actccaattt cgacacaact tcttgatagt cttctatttt gttaatttca
     2821 tcccttaatt ttgcagagtt tctaagtcct tttaaatacc aagcgatgtg ttttctcatt
     2881 tcccttacag ctacatactc tccatgttct tctactgcaa gttttaaatg cttaatggca
     2941 gtacttattt tttcgttaag agttggctct ggcaaaatct cgcctgtatt catataatgg
     3001 tttattcttt taaatatcca agggttacct tgagcacccc tacctatcat tatagcatca
     3061 caaccagttt tgtctaacat atttattgaa tcttctattg taaatacatc accatttcct
     3121 ataacaggta tactcaaatt tttcttaatc tctgcaatta tatcccagtc tgctttgcct
     3181 gtataaaact gttctctagt tcttccatgt attgctaaag cacttattcc acaatcctct
     3241 gctatctttg ctatttcaac agcatttaca ctattatcat cccagccttt tcgaatcttt
     3301 aaagtcactg gcttttttga gttcttcact actgccctca atacctcttc tgcaagctta
     3361 ggatttttca taagtgctga accatctcca tttttaacta cttttggagc tggacacccc
     3421 atatttatat cgagaatctc atttgaataa tcattcatta tctctgctgc tcttcccata
     3481 aactctggtt ctgaaccgaa tatctgaact gctacaggat gttcttcctc ttctatctta
     3541 agcatttttt tagtattttc atcatcatag caaagtgcct taccatttat catttctgta
     3601 tataaaagcc cacatccctg ttctttacaa attaatctaa atggaaggtc tgttacacct
     3661 gccataggtg atagaaaaac tttatttttc aattctaaat ttcctatttt cattgttttt
     3721 ctcctatttt tgcctttatt ttgtgattta actttaaatt cttatttcct cattataata
     3781 taccacatat agctagaaat ctatcataaa aagggaattg gatatataac ccaattccct
     3841 gtatattaat tattcttggt ttttttcata tattattctc aatccttcta gtgttaaaaa
     3901 accatctaca taatctatac ttttcgtctc tgaagcaatc agtttagcta atccacctgt
     3961 agctataacc ttaacatcat cacaattcaa ttctttcttc atcatactta ttattttgtc
     4021 aactaaacca acatatccat aaattattcc agattgcatg gctgatacag tactcttaca
     4081 aatagtcata cctggcttag ctaattctac tctaggtaat ttagacgcac tttgaaataa
     4141 cgcctcacta gatattttta ttcctggtgc tattgttcca cccaaatatt cacctttttc
     4201 agagatagca caaaatgtag ttgctgttcc aaaatcaaca agtatacttg gtgctccata
     4261 cttttctatc cctgctacag cattaactat tctatctgcc ccaacttgtt ttggattatc
     4321 atattttata tttagacctg tttttatgcc tggacctact attaatggtt gttttttaca
     4381 gtactttata caaaagtttt caagagaatg cataacattc ggaactacag atgatattat
     4441 aacatcatca atatcactta tattcacatt atcataatca aataggttac ttatcaggat
     4501 tccatattca tcagacgttt tttccctatc tgttttaatt ctccagtaat taactaattt
     4561 gtcaccttta tatataccta aaaccatatt agtatttcca acatcaaata ctagaagcat
     4621 atgtcattaa ccttctttct attttttgct tttcttaagt acagtggcaa cagcagctac
     4681 tactaaagcc cctactattg cttctggcac cccatttgct gcggcaagag ccattattac
     4741 ttttcctgct gaaccacttt tacccattgc atgaagatac ttgtcagcat ataagacata
     4801 tatcattcct aaaactccag ctgtatttaa cagagaacct attgcccctg taattaaacc
     4861 tgatatgtat accttatttg tagccttaat tattaactga tatacataat aagctaatac
     4921 acctatcata actcttggta atattgaaac taatggattt ataaaaacaa aagatgttac
     4981 tgttgggttt gttatagcat ttaagagact tgatatccca aatataaatc ctattgctgc
     5041 cccaactatt ggtccttcca taatagctcc aataataact ggtatgtgca ttatagttgc
     5101 ttttgtaggt ccaactggaa taaatcccaa tggagtcata gatagcataa tagaaatagc
     5161 tgatagtact cctattatag tcatctttct tacatttact tttcttactg atgcacttgt
     5221 gttcatcata aaaaatacac ctccgttcca gctcattaag atgccggact acttaatttt
     5281 ttaatttttt tcgttttttt cttataacta tgagtcaagc taccattgta ggatgcccgc
     5341 tctaacaaga ataattttat caagatgagt tgccattgtc aacaatttga caagaaaaat
     5401 atgctaattt catcatattt ctcgtttttt tactatatct ttatatgcca ttatattttc
     5461 tacactatcc gcttttttta ctgcatttat tatacttttt tccacaacct ctacagccaa
     5521 tgaacctact aatgttatat ctgcttctat ttctcctgtt gatattgtga atattgtatc
     5581 tccatcatga ggtgtatgga taggaaaaat agacttagca tatccatcat gtcccatttg
     5641 agatatcttt ttacatccag ctttggttaa cttagcattt gttgcaatta tcccaattgt
     5701 agtattatct acactaaatc cacctttact ttcaccttgt ttcattaatt tatatgtatc
     5761 aattgtttct gtccttttat catttaaagc accagctata accttaccat tttcataaac
     5821 atctccaaga gcatttacag caattaatgc agatacaatt agaccattat ctaattttat
     5881 agaatagcta cctattccgc ctttcatagc atattcctga cctcttatct ttccaacagt
     5941 ggctccacaa cctgctccat aatttccttg atttaaaata tcagtatttg caacttcaca
     6001 agctctataa cccatcttta aatctggtct acatttaaag ctccctactc ctaaatcaaa
     6061 taaaactgct cctacaacta taggcacttt acctacacct acatcaaagc caattccttt
     6121 ttcttctaag tatctactta caccacaagt ggattcaaga ccatatgcag aacctccagc
     6181 caaaactact gcatgaactt tttgaaccat atttacaggg tctaataaat cggtttctct
     6241 agtccctgga gcagagcccc ttacatcaac accgcaagta gctccttctt cacatattac
     6301 tactgtgcac ccagtaagac catttctatc ctcaatctgc ccaaccttta agccttctat
     6361 atccaaaata ttattataca tatccttttt ctcctcttat agaaacttcg cctgaaataa
     6421 tttcttttat acttccatca cttctttgaa ctaataaatt tccctcttca tttatgtcta
     6481 aacatttcac tagttctcta ttatctcctt ttaatacata tatattttta tctataattg
     6541 cagaataatc tctacatatt tttaaggttt tttctttgga attatttaca gtataatcta
     6601 aatataattt ttcaaattca gtcaatacat tcctaacaat atctactctg gataacatat
     6661 atccttcttt atacaatgat gtggctttgt cagctatttc ttgttcaaaa tccattattt
     6721 taacatttat tccaattcca agaacaacat aatttgttct ttcaatttca gcagataact
     6781 ctgttaatat accacatatt ttttttccat ttaaaataac atcatttggc cattttatta
     6841 atgtatttgc tcctagattg tttaaagctt tcgttacact tgctcctgct attaaagtta
     6901 taaatggagc tttgaatggt tccatatctg gttttaatat aatactcatc cagattcctt
     6961 catgaggttt tgaattccat cttcttccca atcgtccttt ccccatatct tgatgttcac
     7021 ttaaaatcac agtcccatct acactattta tagctatcct tttagcatgt tcatttgttg
     7081 aatctatagt gtcaagatgt tcaatatttt ttccaataaa atctgtatct aatttatagt
     7141 aaatattctg tggacttaat aaatcagttg gattttccgc taatcgatac ccttttttat
     7201 ttacagattc tatattatat ccctcttctt ttaaggcttt catatgtttc catatagctg
     7261 cacgtgacac ccctaattgt tttgataatt cttctcctga aataaattca gatttattat
     7321 ttaatataat ctctattatt ttatctcgca tatttcacct ctaatatgac ataatttcat
     7381 gacttatttt cttctaataa ataattatac attattaata tacttttaat actattaatt
     7441 ttaaatttcc tttttaatta ttataatggt tgttttatat tttatattga tgatttatat
     7501 aattttacat tttatttaaa taattttaaa ataataaaaa aactgaaagt atcaattaaa
     7561 ttaatacatg caaatataat ttgcttatta atttaatatt atactttcag ttttagtatt
     7621 taatccacat cagataataa tcctaaaatg accaaggtct taaaactatt tatctttatc
     7681 agatttatct tctaaatttt tatttatatc aataacattg tttctttctt cttcatcttt
     7741 ttctaatttt atagttaatt gttcttcaac ttctttagaa gaattttcct ctgtaactgc
     7801 attttcaatt tcattcagag gtaactcttt attaaatgct tttacaaatt gttctgcatc
     7861 taaagtttca tatataagta aagcttgtgc tacatactct aatctatcca tattttcttg
     7921 taatatactt cttgtcttat catatgcacc atcaactata cgctttgttt cttggtctat
     7981 ctcaaaagca acttcttcag aataatttct cttacttgaa aaactattac ctaggaatac
     8041 ttcatcatca ctgtcaaatg acattggacc aagtttagaa ctcattccat actttgttac
     8101 catacttctt gcagtagctg taactctttc taagtcattt gaagcacctg tagatacatc
     8161 cttaagtact agctcttctg ctactctacc accaagtaaa actacaatat tttctttcat
     8221 ttcatttttt gtagcataga acttatcttc aacaggaagt tgcattgtaa accctccagc
     8281 tcttcctctt ggaactattg taacttgatg aacaggactt acttcttcaa gtacatgggc
     8341 acaaactgca tgccctcctt catggtacgc tgtaagtctt ctttctttct cacttattac
     8401 tttcgatttt ttagccacac ctgctataac ctttgttata gcttcttcta tagtttccat
     8461 cttaattttc ttttctcttt ttctagctgt taatattgca gcctcattca ttaagttctc
     8521 tatatcagca ggagtaaatc ctggtgttct tctagccaag acatccattt ttacatcatc
     8581 acttaatggt ttatttcttg agtgaacttt aaatatagcc tctctacctt ttacatctgg
     8641 tgtaccaaca acaacttgtc tgtcaaatct tcctggtcta agtaaagctg ggtcaagtat
     8701 atctggtcta tttgtagctg ccatgattat tataccttga tttaccccaa atccatccat
     8761 ttcaactaga agttgattaa gagtttgctc tctttcatca tgacctccac caagacctgc
     8821 acctcttttt ctaccaactg catcaatttc atctatgaat atgatagctg gagcactttt
     8881 cttagcctgt tcaaataaat ctcttactct tgaagcacca accccaacaa acatctcaac
     8941 gaaatcagaa ccacttatac tgaaaaaggg aactcctgct tctcctgcaa ctgctcttga
     9001 tagatatgtt ttaccagttc caggaggtcc taccataagc attccttttg gtattcttgc
     9061 acccaattca atatatttct taggattctt taagaaatct actacctcct gcaaatcttc
     9121 tttttcctca tctagtccag ctacatcttt aaatgtaact ctagtttttt catcatcttt
     9181 atgaacctta gcttttgatt tgccaaaact cataaccttt ccgcctccac cttgagactg
     9241 attcataaac acaaaccaaa taatcaccat gaaaaatatt aacagtaatg taggcaacat
     9301 ttcgacaaac caaggtgtag atggcttagc ttcaccacca aaagttagtt tcccagcctt
     9361 agcttggtct aacacctcat ctgcaagttt atttcccatc acttctgttg gaacatatga
     9421 cttaaacttt gtatttgtat cttttatagt accttctaca gaagtttgat ttacaaagta
     9481 taatcttgat atattctcat ctgttaattc tctatatact tcagagaatt ttaaatcttt
     9541 tattttttct gtaggcttac ctgagaattg aactatacct actataatta taaaaacaag
     9601 tagataaaaa cctgcccctt ttagcaactt attcaatata gaagccctcc tttcatacat
     9661 tgctttgccc tataatttgt taaaattaat ttttaatact tcttttgtgt tttcatcaat
     9721 tttataattt tcactgattt tataatctcc aacacatatt attccttcac tatctgtgat
     9781 tataggaatt ttacatcttt cttctcttgg gatttttaaa tctatgaata aatctttcac
     9841 ttttttactt cccatagcaa gctttatctt atcaccttgc cttctgcttc ttattactat
     9901 accccctttt accttattaa aatcaaaccc tttagatgaa ttgtctaatt tcatactctt
     9961 atacctatct atgctcatta cttgggtctc aataatttgt tttaattctt ttattttaat
    10021 aaacccatta ctaggtacat tgtaataaaa ttcaatttcc tcattaacaa tttcctcatt
    10081 cgttaatatt atactgtctt tttttctata aacaaatata tttcttggaa gagttaacat
    10141 tttatttact tttgaattat cttctaaaga cattatatcc tctatatgtc tttggtcaac
    10201 aaaattagta tctcctagta tatgttttat ggaatttcta agaactctaa ctttgatagc
    10261 atcatgtaag caaacaaaat cgtctaaatt tatctctaca aagccttttt cttttatatt
    10321 tgaaacttct ctaaatttag cttctgcctc tttctcaata taatcgttat cactttttaa
    10381 actattgctc atcctcacta tagattctat tacatttgaa ttaaaattat ccttcatata
    10441 tggtaaaagg tctaacctta tcttatttct agtgtatata ttttctaaat ttgttttatc
    10501 tatcctcgga tttaaattat aagcttcaca atactcctct atctcatttc tttccacgtc
    10561 taatatgggt ctaattatgc aattatctct aatataatca attcccttta agccttttaa
    10621 acctgttcct ctcatgatac gcatcataac cgtttcggct tggtcattga gattatgacc
    10681 tattgctatt ttattggctt taatcttatc cttaatttca taaaacattt catatcttag
    10741 cttccttgct ccttcttcta atgataatcc ctcattttca caatattttg gtacattaat
    10801 agactttaca aagaatataa ttcccatatc ctcacaaagt ttagatacat ataatgcatc
    10861 tttttgagct tctatccctc ttatttgatg gtttaaatgt gctgcatata tttctatatt
    10921 aaaatctttt tttaatctat ttagtacatg caataaacaa actgaatcag gtcctccaga
    10981 aagacctaaa actattttat cgcctttttg tattaaatta tgtttattta tagtacttag
    11041 taccttatca aatatcatat ttatccctct tacacttagt tttattcact tttaagtata
    11101 ctttctataa aaaaacgcta aacccttgta tcaattttaa gaaattaaag tttaatataa
    11161 atataataat accaaataaa ttaaataaaa ggtagttttt tcactacctt tataaaattt
    11221 ctggaaaata gccatagtta ttatttagta cctatgtata tatctatttt attcctacct
    11281 aaaaataaat cttgtacctt tatattatag tctattttca attttatgtg ttccccttct
    11341 tgtatattta tatctaattc tcttgtatca atatcaatta ataaagtacc ttgtggggtc
    11401 acatactttg tagtactact ttctccacat ttaaacagca ttgttgagtt aatagcacca
    11461 aactttttga tactaacttg atttttaagt atctttatag tattagttat ttctatattt
    11521 tcttcttttt ccttgtaaat aacatatatt ccatcatttt tataaaaaat tttaccaaag
    11581 gcagtcatct ctatggtgtc catatttcct ttttcatcat attgtctagt atttatactt
    11641 atttttacac tcaacgattt ttcctccata gattcttcat ataaactagt atttttgtat
    11701 ttctactttt tgaatcttat caattttttt ctttaagaac tctcttgcta tatctgaaaa
    11761 tttttcaggt atatctgata cataatattg ataagttcca tgaatttctg tattgtttat
    11821 aatattttgc acctctaaaa tcttttttaa atcctttgca gtctctttag ctggattaac
    11881 aagttttata tgctctccaa cttcttcacc tattgttctc tttaaaatag gataatgagt
    11941 acatcctaac actaaagaat ctatattttt ttctttcaat tcttgtaaat atatctttgc
    12001 tgtcaattta gccacttctg tatttgccca tccttcttca acaataggta caaataaagg
    12061 acatgcttta ttaacaattt ctatactctc atctatttta gatatttcca agttgtatgc
    12121 ttttgaactt atagttcctt cagttcctat tattcctaca attttgtttt ttgtagaact
    12181 tacagctgtt ctagctcctg cttctataac tccaattatc ggtatatcat atttttcttg
    12241 agcttctttt aagcttcttg cagtagcagt gttacatgct ataactatag cttttacacc
    12301 ttttgttttt aaaaagttta tagcttgaaa agtatattta attatagttt ctttggatct
    12361 tgaaccatat ggtattctgg cagtatctcc aaagtatatt atatcctcat ttggtaatat
    12421 cttcattatc tcttttagaa ctgttaatcc acctaatccc gaatcaaaaa ctcctattgg
    12481 tttattgttc atctttatac aacacctact ttacattttt gtgtttaaca acaacttatt
    12541 tatcgctaaa atttatattt atattcataa ttaatatatt attacctaaa tgattattat
    12601 agtttatttt acacaaaaaa taaaggagct atcttaaatt agaaaataat ttctacaaaa
    12661 agacaactcc tttcactact taaaaattta aatttatttt gcaaacacat gatttcctat
    12721 tctcatataa ggattcaatc tatttatcca tttgcaagtg gctatttctg gattccaaaa
    12781 atatactgct ttattagttg ggtcagttcc ataaagagct tcctgagctg ctttacgtgc
    12841 tgagtctgtt ggtgccatct ttattgaacc atcaagcaca actgagaaag catttttttg
    12901 atatatgact tcttctattg tgtctggaaa tctagggtcc aatactctat ttattactac
    12961 agctgctaca gcaacctgtc cctcatagct ttctcctcta gcttctcctg caaccaactt
    13021 agataataac atcaaatcat tatcagataa atttattact tctttctttg ctatattttt
    13081 ttcttgtttg tttactttct taatattttc tgttggttca tatgcaaata cctctttaat
    13141 aggcataaat ataaaagtca aagctaatag tgtagccatc agtttactcc acctgtccat
    13201 gattaaaacc actccttaca ttcttatttt taaaattttt tctctaaatc cttgactact
    13261 tcaagtactt taaacttaat ttttactcta cttgtttttt ttggtgtatc ttgttttatt
    13321 aacttatatt ctttatcagt taaaacttcc ttcaacttat catctgtaga tttatctatc
    13381 actccattag attcatcaac aaaacatagt ggagggaaca taacacacca ccagtttttt
    13441 ccttcacctt tcccaatgat aatctttaat gctttatatt caccagctgg aagaactata
    13501 ttagaatatt gtttagtagg gaaattgcta tattgtatac ctacctttac actgtaatta
    13561 tatccatttt taagaataat atttttactt atttcttcca gactactata ttcctttttt
    13621 ataatagctt cactttcttt tatacttttt gaattttgaa gctttggctg taaatatttt
    13681 attacttcat ctctaacttt tagtttaagt tcttggtctt catctgtatt gctgtttgct
    13741 ataacatgaa atcttattaa tttatcttta taatcttttg atatattgtc tacttttttt
    13801 acctcaccat ttattactat tgtcattatt gatatgactg atataagact taatattaat
    13861 agtcccaatc taatcttaat ttttctcatt gaaaattccc cctcaagata aatttatatc
    13921 atcaattatg tccaattaaa tttaatttat tcataatatt ttttaatact aataaaaaaa
    13981 gctatcttaa aatgattaca atcattttaa aatagctttt taaaccttat attttataag
    14041 tctttactaa tcatagcctt atctatcata aactgctctg ctttttctat atgctctgta
    14101 gcatacttct tggatagctc actatcacct ttaattatag catcaagtaa atatttatgc
    14161 tcttcaacaa tattatgtga tgtatcataa gcagacatat aggtaactct aaatctatat
    14221 acttgttccc acaatgagtt tattaattgg attaattttt tattattagt tgctttaaat
    14281 atacattcat gaaattctac atcaagtttt aataacgcat ctacatcgga ttccaaagtg
    14341 ctttttttaa attcttctga tatatcttta agctttttaa tatcttcatc actaattctt
    14401 tcagcggcaa gatatgctgc taagccttct aggcttgctc ttacttctaa tacatccata
    14461 atatccttta atgacatatt tgccacatac gcaccttttc taggcaacat aacgactaaa
    14521 ccttctaatt caagctttct tattgcctct ctcactggag tcctacttac tcctaattgt
    14581 tctgctagtt gcacttccat tagcctttga ccaggcttta attttccttc taagattgct
    14641 tctcttaaat tctcaaagac tacatctctt aatggcttgt aattatccaa gttcagttta
    14701 gttaaattct ccacaaattt caaccccttt ttgactgcta ttaacaacat acacctgttt
    14761 atattttttt aataattctt tttttccaat tagagcatct tctttatttt taaatagtcc
    14821 aaaaactgta ggaccacttc cactcatcat agaacctaaa gcattatttt tcatcattac
    14881 ttgttttata tcacttattt ctttgtgttt acctatagta acattttcaa gaatatttac
    14941 catgctttct gatacagctt ttatatcttc agattttaaa cactctatca aatatttatt
    15001 atttggtctt tttttaatat tattcaaatc taatccttga taaacttctt tagtagaaac
    15061 gaataagtct ggtttgcata tcaatatatt tgtatcacat ggtaaacctt tgatatttgt
    15121 tagcttttct ccaatacctt gagctaaggc aggtcttcct gatatacaaa aaggaacatc
    15181 tgcgcccaaa ttaagtccta tttcttttaa ttcatcttca gataatctaa gttcccatag
    15241 gtgatttaaa cctactaata ctgcggctgc attagaactt cctccagcca tccctgctgc
    15301 tacaggaata tttttttcta tgaatatttc tacacctttt tttatataaa atttattttt
    15361 aagtattttg gcagctttat aaacaatatt atcttcatct aaaggaatat ctaaactagt
    15421 gctttttacc tttatttcat cttcgtctaa ttcttttatc tttactatat cgtataaatc
    15481 tatcgtttgc attatcattt caacaaaatg ataaccgtct tgcctttttc ccaagacatc
    15541 aatggataaa tttatttttg ctctactctt tagtcttata aaatccattc tatcctcctt
    15601 gtttaaagaa taatgcatca ctaacaataa tattatacta caaaaaataa aataataggg
    15661 tattataata ccctattata cttaattact ttaaattttt taatattaat ctactaatac
    15721 gacttttttt tctaaaataa gacacttacc aggcttaatt ggttcatcaa gttttatatc
    15781 attaagttct gcaatttcat tttctgttgt attgtatttt tttgctatat tccagaaagt
    15841 atctccttct ttgcatatat atacgattat gctaggagct ttagataaat catatacacc
    15901 ttggtcttct cccttgataa taaaattttc tgctttctta tctaatgctt cagtaaatct
    15961 cttaattttg ataatcaagt ctatctggtc cctattcaag tcaacttcga ctttatcaat
    16021 acatgctgta ttaaacacag atgctgtatc agttagatta tccatttcta tatcatgttc
    16081 aaatggaatt tcctcactaa ttttataaac caattttaaa ccttcaactg ggacaaataa
    16141 aatttcaact tttatgattc cttgtataat gcttttattt ccttcaatat aactattttc
    16201 tatagacatg gttggacaaa cgcttacaat atcttttatt tgtatatcat cattatcatt
    16261 tcttatgcct tctctaacca taaatgtttc ttcactacgt cttaaagtct tatttaattg
    16321 aataggtttg tggtcaaatt ttattatctt ttgaggcgaa tatgcatctt gtaatacttc
    16381 tcttgtaact tcatctgtaa cttttacctt acaacaagcc atacaatcta tttctaaaag
    16441 acctgtgttg ctttcactat tttgcttgaa aatatgatta aaatctgaca tagacaacaa
    16501 tacttcttca gtcataccat cactaactcc agggacttct acaaattgag taaactcaat
    16561 acctactcta tccaactcta caagttcacc ctcatatgta caagctaacg gatttatttc
    16621 taatactcca ccaataataa ctttattgtc agtcactcta ctttctttta ctttaacaca
    16681 tggatttaaa cttattatag attgaatttc ctctgtgttt attgttatag tatctcttat
    16741 agaactttct gctttttcta ttccaactat atcttgaaaa caaatttcct ttctgtgctt
    16801 ttgtatgcct tccacttgcg ccacatcttt tacaatgtct aacctttgct tttcaaacaa
    16861 acttcccctt atattcataa gagctcctac tttaatcttt ctctcattca ttatagtaca
    16921 atccatatgc tctacttctg aaaataacat gtattccata tcttgaataa cattgtcttt
    16981 ttctattact tcgtttatat ctacttttcc gtctacatta gaaatagtat ttttatcatc
    17041 tgcaatatag attacattat aattaaaact acctctgcaa agtatcttcc catctgccat
    17101 ttcaattttt ttaagtgata tgtatccttc tgtttttaca atttcatata catctaattt
    17161 tttgtctggt acaactgcct ccgcttcaat gaatgtttga aattttccaa agtctattct
    17221 attgtcaact ttaattacat ctttaattaa ttccatagtc tacctcctta agtattataa
    17281 aaatatcaca ctatatttat atttaaggag caaaaaaaaa attacaccta tttttaaata
    17341 ggtataattt taacttattt gtaatttatc ttgatctcta aaaacttgca actttacatt
    17401 agatgttaat aaatctgagt aactataaga tactctagga tatccattac tttcatcatc
    17461 taactttata acaaacacgc ttggataaac tttttctagt ataccctctt ttgtaataat
    17521 ctgctttctt cctttattag ctttcaacaa tattttcttt cctatatgtc tctctaagct
    17581 tactcttatc ttatctagag tttgaacagt agccacaata tcaccttctt taacgtttat
    17641 atgtttacat aatatcacat ataaacattt ttgtcaaatg tttaaactaa ttattttata
    17701 tattttttgc aaaaatgtca atactatttt tgaagatata cagtataatt atgttaattt
    17761 ttgctaatgt aatattgttt ttggcattgc ccatctaaat tttcctcttg tttcagaacc
    17821 accaattccc ttaacacctg taatagtctg ctctataacc tcatcaatag gaactactac
    17881 atcaattctt gaacaagcaa ttttctccac tataaaaaca gggtctaatg catgtatcat
    17941 tattcttcca ggcgaacttg catagtttgc tcctgcactt attatttttt cataatttga
    18001 ttggcatgct cctgcgaata tgacaagatt atccaaattt ggttgccatt tacgtgcttc
    18061 ttttactgtt ttaacaaaat ttgaagagtt tctatagtta ctcatatctt ctatatctcc
    18121 tctttttatt gtcattgcat catgacctgt tattactagt atatccggat tatatttttc
    18181 tagtagtgct cttacttctt tatactgatt ttgctctgaa acagctacac ctactgcagg
    18241 tattccaagt tgagcatata catccaagca aatttttaaa tattctttat ctccatctat
    18301 ctgtagtact tttccaggca ttccatatgt gttaggattt aattgaagtt ttggaactgc
    18361 tctagtcatt cttttttgtc tttctttagc ttttcttact gacttatata ataaattttc
    18421 tacattttta tctattaata tatcttttat atcaggtgct tttactaatt ctaggtcatc
    18481 aatataagca tctgctatta ctctaaatgc aattcccttt agtatagcta ttttctcatt
    18541 attttcatct acaccaaaag agactatttt aaatactata tctttgttat gtgactttct
    18601 agctacaatg tctcctacct tcatagttaa acctccaata aatttttatc ttagtttata
    18661 ctatgttcga tataaaactt tgtttcctaa tgtcaaaaaa acatttctta taatacttct
    18721 tttatcactc ttaaagcatt tttatagtat atcttttcta tatcatcttc actaaaccct
    18781 tcttttttta gaggttcata cagtttaccc atctgagata tatcttcaat ctcaactttt
    18841 gaatctattc catcaaaatc tgaccctaga gatacaacat ctattcctcc aacatttaca
    18901 atatgtttta tgtgtctaac catatcttca agtttacttt cacttttatc acttaaaaat
    18961 aagtggaaga agtttatacc tgtaactcca cctttatttg ctaatacttt aatcatatca
    19021 tcagttaaat ttctagaatg attcatcatt gctcttgaat tagaatgtgt agcaattatt
    19081 ggtttagaag aaatctttgc aatttcataa aatccaccat ctgaaatatg agaaacatca
    19141 accaacattc ccaattcatt catcttatgt accacttctc ttccaaaatc agtaagccct
    19201 ttttctctat attctacttt attatgagga aagctcaatt cattcacatg attccaactt
    19261 attgtcatca ttctaacccc taagtcgtaa aatttcttta aattttctaa tttaccttca
    19321 agtactgcac cttcttcaat agataaaaga gctgtaagtt taccttctga ttggtttttc
    19381 ataatttctt catagttagt agctaaagct atttcatcac tatttttttt catttcctca
    19441 tgaaatttat ttgccatact catgcaataa tcaaaaggat gttttacttc ttcagtatca
    19501 acaaaaagag caaatgtttg agctaatgaa tccccctttt tcaatctgtc tatatcaaca
    19561 gaatatttat tagacttaag ttcacttgtt tctacatttt ccattaattt tgctatagta
    19621 tcacaatgta aatcaataaa tttcatattt ataccccctg tatattttat atttatatct
    19681 aaaaatagaa atagttgtcc ttatagacaa ctattgacaa ctatttttta atattctttt
    19741 taatattact taatcttatt cttcttttaa gttaggtatt acttttttaa cttcttcaat
    19801 ttgatgccat gttttatcta tattattttc actaatatat ttttttaaat cagttaaaga
    19861 tgtaaaatct ttaactgata aattgtcatc atcatctgca aatcttaaaa taaacatgag
    19921 actcccttac tttccacaac atttcttata tttttttccg cttccacatg ggcatgaatc
    19981 atttcttcct atttttgctt ctttaactat tgttttagac ttcttataag tctttattat
    20041 ttcttttctt tggtctgctg gtagtatatc ttcccaacct tgtaaattat atagccaatg
    20101 agcctcaaca gttaccatat tgttatataa tttttcccaa tctattttta tagaaacttc
    20161 tttatcagct tctacttctt ctaaaactat atcttctttt aagctttcac ttattccatc
    20221 tatgaatccc atgaaaaatt cattagtagt tccaaatttt tcagccaatt cacttacttt
    20281 accttcaaca acatccattt tattatttaa tatttcattg tatattccag cttcaacttt
    20341 caaatattct tcccagaatt ttacttctgc ttcttgactt tcatgattat cacttaaatt
    20401 tctccattca gtatataaac tcatactttt tcctcctaag tatacctata tttttatagt
    20461 ttttatagta taaaatcttt tagttagatt ggaagtttct tctttttgat tataccatat
    20521 tctatttaaa gttaactctt atattatatc ataattaaac aattctttta atacttttat
    20581 agcatactct tggtttataa aataagggct tgtcgcaatt gttattactt tggctttttt
    20641 aattaagttt tttatcttgt aaaatttgag ttcaaatggt atatagtcca tatcacttga
    20701 aaatatatcc aaatctatgt caagaacaaa ctctgattct acttctaaat caaatccata
    20761 tagactatct attattatta attctgaaaa tatgttatat tttaaagcag gttttataaa
    20821 acttcccaca tttaaaactt catttgtata tctaaataca tcttttagat tatttacatc
    20881 tacatcatag ttctcaggtt ctctagtatc cttatgttgg tctacatgca ctaacttgca
    20941 tccttttgta aactcaccct ttaacaaaga ttttgcccaa aaataaaatg catggttatg
    21001 attgtcaaac acatatatct ttttatttcc caggacatat tcgaccatat tctccaatcc
    21061 ttttgctttt atttccttat tttcatctac ttcattaaat actacctcat tgcctaacct
    21121 tacatcattt aatgtaccat ttattagttt tggaacgtat atgctcttat tttctctttt
    21181 atcatatgaa aatacattat ttcctactgg tttttctata taaaatccac tatattcata
    21241 attttccata aaactttatt tcctccattg ttatataaat atataagtta taattttaaa
    21301 aattaaaatg cacttattgt aaatcatatc atctattata gaaaacatct tatataacta
    21361 aatataatat cttctctcaa tatgtattat acaataagtg caaaatatat ttatataaaa
    21421 tatactacat tcctataaca aaaccatcag cttcaattgg aaggtaacta actcttctat
    21481 tcaactccac ctttccaaaa aatactaccc aatatggtct aaaaatactt tcaattttaa
    21541 ctatttcaat ctctgggatt cctcccataa acctatgagt taatctcata gccattttat
    21601 ttgcctttgc ttttactctt cgttcgtcct tatcactata ctgcacaata ttacaatcca
    21661 cattttttac aacttcatat gtaggaaaat cttgtactat actaccagaa cctgtagaac
    21721 cattcactaa aatttttaat tcatttatat cattagaata tctcctattc ttgaaaatgg
    21781 atttcgtttt gtgaatttta agtgtgataa ttttaaactc tatataatga agtctaactt
    21841 catgagcata tttgttcttt aatattagct tactaaataa agaatttttt ccacttttag
    21901 ctatctctat agcttcttct tttgtaattc ttataggtaa cgctcttata tccataaaat
    21961 cattctcttt cttatttttt tcttgtcatt tttaaaactc tatatatata atcaaaaaat
    22021 attacaatta caactgccat aactccatat ggaattattt ttccaactat aatatcattt
    22081 ctagtcaata taccataaaa tataaatcct gttattaaaa agtacaataa tactagaaaa
    22141 ccgtctattc caagccattt tatttctaaa tcttttttta ccttagacac taatttagac
    22201 atagcagttt ctccttttat agattatttt gtgtatttaa attttcacct attctattac
    22261 caatcatcca tgcaataaat gaaaaaggta tagctatcac aagaggctct aactcaaagc
    22321 tctgaactgg aaatatttta aaataaccaa aagtcactcc accaacaatc attcctaaaa
    22381 taccacccat agatgttctt cttccatttc caagaagccc acctactata ggtacaaatg
    22441 ccgaacccat ccaaatactt cctacaaata tacatgcatc atatacattt tcaaatctaa
    22501 atgctacgct aagccccata actgctgcta aacatacacc tattctagta aactttacta
    22561 attgtttctg agataactct ttttttgtaa ttcttccata tatatcattt gctaatgtag
    22621 ccccagctgc tagataatag ctatccaatg ttgatattat agaacctaaa attcctatta
    22681 taaatattac tcttattcct tgtggtaatg tatctaaaac taaagttata tatccagcag
    22741 ctggagccat ttctggataa gtagctctta ctataagtcc tgtcatagtt aacaccatgt
    22801 caaaacatat ccatagtgaa aaacaagtta ataaggctct tcttccaacc ttaggagaat
    22861 ctgctgctga aaatctttga taaaaagttg ggtctgaata agctccaaaa gaatatacaa
    22921 ttaacatcaa tgctttcata gctgacatac cttttgtagg gtgcataagc tgtggtgcat
    22981 ctttgagtgc ttctgtaaga ccttgaaacc ctcctatttg ttcaaattga cctggcataa
    23041 ccatagttaa accaaatacc atacaaaaaa acaataccat atctgtaatg gcaactccca
    23101 taagcccacc aagtactgta agtacaacga ctactcctac cattattaat cctattaatt
    23161 ctttatctat tttccatgct acgttaccta tagttcccat agcagttatt tctccaagtt
    23221 gtgcacaata tataaacatc aaaattgccc caattatagc aacatgcttg ccataaaaac
    23281 tttccagtaa atcaggaact gttatattgg ttcttatatg aatccttgga ccaacccaca
    23341 atgctaaagg cattctccca atatgtgcag ctattgacca aactgcccat gcagatattc
    23401 catatatagc cgcatactct acactaccta ctgtaccaac tcctccatac catgatgcaa
    23461 ccaaagttcc tactattaag gaccatggta aacttcgatt tcctaggtaa taactttcca
    23521 tatcttttac atttctagaa aaataataac ctattcctaa taatataaat gcaaatataa
    23581 ctataatccc attttcccaa aatgttaaca tatctaatct ccacattctt atattttaat
    23641 ttttaacttt aatatctaaa tatgaacaac tctacccatt tccataaaaa taggtagagt
    23701 tatataataa ttataagtat ttaagtaatc ttgttattaa gcaattttat tgtttaaacc
    23761 tttattttcc cttttattta ttttatttaa agcataagta gcaaatatcc ctataactgc
    23821 acatactaca gataaaataa atatatatgt atatcctgtt tgtccatatt tatctaacca
    23881 attacctgca atagtgtaat aaaacgcatc tggagaaaat cctataaatg aagcaaatcc
    23941 aactactgct cctgtagttt ctaaagatat attagactct gaaacagatg caaagtatat
    24001 acctctaaat ccaaataaaa ttacacttag tattatcatg tttattacag ctatccatat
    24061 aaatcctgta ctcttaggaa taaccaagta agcagctgta cttactgtca ttaatataaa
    24121 tcctacaaac attactctta tagatgaacc aactttatct gttataacac cagcaactgg
    24181 agatgctccc atttttataa cataagttct tattattgat aaagcagaaa ctagagtcat
    24241 tggcattaca taaacttctg ataagtatgg acttaaataa gttaaacttg agaatactaa
    24301 ataagttgaa aatattataa gaccaattaa ccaagccttt ggcattgtaa ctacttttaa
    24361 caatgactta atacttaatc cttcatctga tgctccacta tcttttttct cttctactaa
    24421 gaaaaataga agtataccac atattataga tgctgcagaa tataaccata ctacatattt
    24481 gaatcctact gttatttcag caaatttacc aaagaaataa agacctataa aagaaattaa
    24541 tgtacctgat acgcctgata acccttcata aaaaccaaat attcttcctt gctctgactc
    24601 acttccttgc attctaacaa ccttaacaga ggctgagaag aaggttaata ttgttgtaaa
    24661 tccccataat acaaatatta aaagtatcat actatatgat gggaatgtag agaaataaaa
    24721 tccaagtatt cctgaagcaa ttaaagaaaa agttattaat tttctaaccg agaatctatc
    24781 tgcaaaccat cctcctataa aataagatac cattcctact aatccatata aactcaataa
    24841 gttccctatt tgtgtatggc tcaatgataa agcttgttgc attgggtcat aaaatgatga
    24901 tttcatatat ggtaaactat acatagccgt tgttccaaat gccaatatga atattatcaa
    24961 gaatctttta aaattacttt tgttctccat tgattttctc tccttaaaat tttattagtt
    25021 ataactttat tcatataatt ttattttaat ttttctattg cttgtttaaa atcatttatt
    25081 atatcatttg aagattctaa tcctacagct attcttatta gattttcccc attcatttga
    25141 actaatgtat gctcttcccc taagcttgtt gctattgttg caagctctaa agaattcatt
    25201 aatcttctac tagcttggtc tcttgtcaat ccattgatac catccttaag tttaaatgaa
    25261 actacacctc cacctaaacc attcatttga gttttacaaa gctcatattg aggatgtgat
    25321 tttaatgctg gatactttac caattccaca ttctcttctt cctctaaaaa ctctgctact
    25381 gctaatccat tttcacaatg ctttttcatc ctcatatcta acgttttcat accccttaaa
    25441 atcatccatg cactcattgg tggtaaagat gctcctgtaa aacaacataa acttggccat
    25501 ctaactaaat ctattaattc ttttgaacct gctactgctc cccctaaagt atctccatgt
    25561 ccatttataa acttagtaag actatgtatt actaaatctg cacctaaatc tattggtctt
    25621 tgtacaattg gtgtcccaaa agtactatca actatagcta agcttttatt ttcatgagca
    25681 atttctgcta ttgtttttat atctactaag tctaatgatg gatttaaagg actttctata
    25741 taaactattt tagtatttgg tctcattgct tcttttacat tttgtggatt tactgcatct
    25801 acaaatgtaa cttcaactcc aaatttagat aacagctcag acatgaataa tttagtgtgt
    25861 gaaaatattc catctgaact aactatatgg tcaccacttt ttaccaatgc taatatagta
    25921 gatgttattg cccccatccc tgatgtacta gatatggcac tttctgcatt ttcaagcata
    25981 gccaacttag cttctaactc tgtaagagta ggattacact ctcttgtata cacatatccc
    26041 catgtcatac acaactcttc aaaatgctct actgtctttg cagcaaatgt tgctgtttga
    26101 catattggtg tagccaatgc acctgttgtt gggtctgcac tatgtcccca atgtattatt
    26161 tttgtctcta aatcttgatt aatattgtta ctcattttga tttcctccca ttttgactta
    26221 tttatacaac gttttcccta ccaaatttca tcaatttagc ttagtcttaa tacatttttt
    26281 caagtatact tttttatttg ttataatttc taaaaatatg acacttattt tgttttttta
    26341 cagttctaat tataaactct atagtaacta tagagtcaat atgtttttga tatttttttt
    26401 acaatgttta aaatagtcag tacaaaaata ggtatatctt agaataacta aagtcatcct
    26461 aagatatacc tatttttata tcacaatata aataattaca tataagcatt agttttttta
    26521 tcataaatag atttttcaag
//
LOCUS       DAMJYC010000052        24639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJYC010000052 DAMJYC010000000
VERSION     DAMJYC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.053"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     regulatory      104..194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            604..921
                     /gene="flgB"
                     /locus_tag="PC472_002336"
     CDS             604..921
                     /gene="flgB"
                     /locus_tag="PC472_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2727655.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            929..1333
                     /gene="flgC"
                     /locus_tag="PC472_002337"
     CDS             929..1333
                     /gene="flgC"
                     /locus_tag="PC472_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2727656.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1343..1657
                     /gene="fliE"
                     /locus_tag="PC472_002338"
     CDS             1343..1657
                     /gene="fliE"
                     /locus_tag="PC472_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2727657.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            1682..3232
                     /gene="fliF"
                     /locus_tag="PC472_002339"
     CDS             1682..3232
                     /gene="fliF"
                     /locus_tag="PC472_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2727658.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3245..4315
                     /gene="fliG"
                     /locus_tag="PC472_002340"
     CDS             3245..4315
                     /gene="fliG"
                     /locus_tag="PC472_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2727659.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4308..5039
                     /locus_tag="PC472_002341"
     CDS             4308..5039
                     /locus_tag="PC472_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2727660.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYNEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5043..6362
                     /gene="fliI"
                     /locus_tag="PC472_002342"
     CDS             5043..6362
                     /gene="fliI"
                     /locus_tag="PC472_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2727661.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVYATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRGIFE"
     gene            6394..6840
                     /gene="fliJ"
                     /locus_tag="PC472_002343"
     CDS             6394..6840
                     /gene="fliJ"
                     /locus_tag="PC472_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2727662.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSIGKNNDSVQNQKITINYLLALNNSIMDLSEKLDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6868..8073
                     /locus_tag="PC472_002344"
     CDS             6868..8073
                     /locus_tag="PC472_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2727663.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNDKFEDNLYKTTDHKKV
                     KDRRETSKQNVSSDRPENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8091..8699
                     /locus_tag="PC472_002345"
     CDS             8091..8699
                     /locus_tag="PC472_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2727664.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8726..9709
                     /locus_tag="PC472_002346"
     CDS             8726..9709
                     /locus_tag="PC472_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2727665.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9722..9925
                     /locus_tag="PC472_002347"
     CDS             9722..9925
                     /locus_tag="PC472_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2727666.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9925..10743
                     /locus_tag="PC472_002348"
     CDS             9925..10743
                     /locus_tag="PC472_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2727667.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10733..11428
                     /locus_tag="PC472_002349"
     CDS             10733..11428
                     /locus_tag="PC472_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2727668.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11440..11883
                     /locus_tag="PC472_002350"
     CDS             11440..11883
                     /locus_tag="PC472_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2727669.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11898..12272
                     /locus_tag="PC472_002351"
     CDS             11898..12272
                     /locus_tag="PC472_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2727670.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEVLKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12273..12938
                     /gene="fliP"
                     /locus_tag="PC472_002352"
     CDS             12273..12938
                     /gene="fliP"
                     /locus_tag="PC472_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2727671.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12951..13220
                     /gene="fliQ"
                     /locus_tag="PC472_002353"
     CDS             12951..13220
                     /gene="fliQ"
                     /locus_tag="PC472_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2727672.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13232..15046
                     /locus_tag="PC472_002354"
     CDS             13232..15046
                     /locus_tag="PC472_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2727673.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYSVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFNMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15055..17130
                     /gene="flhA"
                     /locus_tag="PC472_002355"
     CDS             15055..17130
                     /gene="flhA"
                     /locus_tag="PC472_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2727674.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17105..18121
                     /gene="flhF"
                     /locus_tag="PC472_002356"
     CDS             17105..18121
                     /gene="flhF"
                     /locus_tag="PC472_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2727675.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDAM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18118..18996
                     /locus_tag="PC472_002357"
     CDS             18118..18996
                     /locus_tag="PC472_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2727676.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNVISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19010..19711
                     /locus_tag="PC472_002358"
     CDS             19010..19711
                     /locus_tag="PC472_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2727677.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19727..20149
                     /locus_tag="PC472_002359"
     CDS             19727..20149
                     /locus_tag="PC472_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2727678.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            20134..20319
                     /locus_tag="PC472_002360"
     CDS             20134..20319
                     /locus_tag="PC472_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2727679.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20360..21133
                     /gene="flgG"
                     /locus_tag="PC472_002361"
     CDS             20360..21133
                     /gene="flgG"
                     /locus_tag="PC472_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2727680.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21154..21915
                     /gene="flgG"
                     /locus_tag="PC472_002362"
     CDS             21154..21915
                     /gene="flgG"
                     /locus_tag="PC472_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2727681.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21938..22819
                     /locus_tag="PC472_002363"
     CDS             21938..22819
                     /locus_tag="PC472_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2727682.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22822..23196
                     /locus_tag="PC472_002364"
     CDS             22822..23196
                     /locus_tag="PC472_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2727683.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23228..24631
                     /locus_tag="PC472_002365"
     CDS             23228..24631
                     /locus_tag="PC472_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2727684.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
BASE COUNT        10199 a         2632 c         4618 g         7190 t
ORIGIN      
        1 ataatatttg taattattgg taactaaata taaaatataa aatatttgat tataaacaaa
       61 aaagatattt aaaaaatttg ttatttattg tatacttaag ttaaaactaa ataggcaaat
      121 ctagagaaat ctagtgacgc aaagctatag ggactaaggt ttatatacat gaactatgtc
      181 agccagttgc caaaaagagt cctaggtgta ttgtatacct aagaaaagtc tagaatgact
      241 gcctttttgg cagtcatttt gttatgaaaa aatcacttta tttcagtaga aaatacctat
      301 aattaacata ctttaccaaa ttataacata aaataaagaa aaattatctt aaaaagttac
      361 tattttacaa aaaagagcaa cttttcgaag aaatatttaa atacatatta aaattttttt
      421 atattttcat gggacttcat tgaaaaaata ttagttttct tactaaaatg atacattatt
      481 gtattaatgt aatggaaatg cgattaattt aaatgaataa agaagtcatt ttttgtaata
      541 tagcaacttc atttttttat taataagcaa aataaataaa atatattttg aggaggagtg
      601 agtgtgaata catatgacat tataaaaatg ggattagatg caactaattt aagaagtcag
      661 acaatagcaa ataatatagc aaatataaat actgaaggat ataagagaca ttatgtaact
      721 tttgaagaaa cattaaatga tgctatgccc aaaatagaag aaaaaacaga taattctaaa
      781 ggattaagag tggatggcaa taatgtagat atagaagaag aaaaagtaaa ccaagcaatg
      841 acaacattac aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgactaaa
      901 tcaataataa gtgggaggta attaaaaggt gagtgtgttc agtggaatga gaatatctgc
      961 tagtggatta acagcagaaa gcttgaaaat ggatatcata tcgtccaatg tagctaatat
     1021 aaatgccaca agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga
     1081 aaactatgat aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa
     1141 atcacctctt aaaaaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt
     1201 agaatatcca aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc
     1261 atatgattct aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga
     1321 aataggaaag tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc
     1381 aaagttagat aatttgattg gagaaacaaa accattaact cttaataaag aagaaaaaaa
     1441 gaattttgaa gatgtaataa tagatactat agataaagtt aattttcaac aagtgacagc
     1501 agatgatatg aaagaaaaat ttataaaagg cgaagatgtt tctatgcatg aagttatgtt
     1561 aataggtcaa gaagcacagc tatctatgca atatttaata gaagttagaa ataagattca
     1621 tgatgcatat caagaaatga ataaggtaca aatttagcct tttgaacagg agtaataatc
     1681 aatgatagcc agttttaaaa ataagattaa agagcaagtt gctaaatcta ggacaaagat
     1741 aagttctcta aaaaagggaa ctaaaatagc attaggtata ggggttattg ctatatgttt
     1801 agcgctagta tttacagcaa tatctgcgag caaaaataag tatgcagtct tattttcaga
     1861 actagatgtt caagatgctg cagatatatc agcagaatta gagtctaaaa agatagatac
     1921 taaggtaaag ggaagtacga tttatgtacc tagaacagaa gttgataagt taaggctgga
     1981 gttagcacca agtcttacaa gtggttctaa aggatttgca ctaatggatg atagttcatc
     2041 aataggactt actgatgaag agtttcagat taagaagcaa agaatgatac aaggtgaaat
     2101 agaaaaaaca ataaagagtt ttccacaagt ggaagacact agagtacata taacatttgg
     2161 agagtcatca gtatttgaga aggaggctgt tccagggagt gcagcagttt atttgatgct
     2221 aaaaccaggg acgactttag aaaaaaacca aataaagtct gttatggcac ttatatcagg
     2281 aagcgttaca aatataccag agaaaaatgt tcaggttatt gataacaaaa tgaatttatt
     2341 atctgaaggt attttggagg atgaaaatgt taatgaaaat aatcaaccaa taaatagtgg
     2401 cattagtgtt gataaaaata aggcatctga acaagcattg aataaacaac tagaaaagtc
     2461 tattttagaa atactagaac caatatttgg taagggaaag gtgaaagcta cagtaaatgc
     2521 tgatttaagt tttgatacag ctgaaaaaac tgagataaaa attgaccctg ataaagttgc
     2581 aataaaagag acaaggtcga aaaattcatc taaatctgct acaaataatg ttcaaggtgt
     2641 agatgctaac atgaataacc aaggaaataa caatggtact aatacagaag atagtttaga
     2701 tgagagtttt gagtatgaaa caggtaagat tgaaactcat actatagttg cgcctggaga
     2761 acttaataga ttgacagctt ctgttgcaat agatggaaaa atagctaaag gtgttcaagc
     2821 tgatgtagag gatattgtta ataatgctat aggtatggat ggagcgaggg gagatactct
     2881 tagcgttgtt tcaatggtat ttgacccaga aggtaagaaa gaggctaaac aagagctgga
     2941 agagattaaa aaagaaggat ttaaaaagaa cataataaaa gcagttattg gagtagtggc
     3001 agtagtagca attggagcta ttggatactt gatatataag aaaagaagtg aacgagaaga
     3061 tatggattat gaagatgaag attatatttc taaatctgat tctgttgata gaaaagataa
     3121 tcctctatta ggagatgacc cagaactgac attagaggaa gctgttaaaa tgtatgcaga
     3181 agaaaagcca gaccaagtaa cagagataat aaaaacttgg ttaaatgagt aagggggata
     3241 gaaagtgtcc acggaaatta caaatgttaa tgcgtttgac ttaagtgata taaatgagag
     3301 tgataagaaa gagaagatag aaggtgaggg tttaaaaaaa gctgctgtat tattaatgac
     3361 attaggacca aatgtttcta gtgatataat aaaaagcttg cctgataaac aagttcaaaa
     3421 aataggtttg gaaattgcaa atatttcttc aatagcttca aaagaaagaa gagaaatctt
     3481 aaaagagttt gtagaaataa atagggcaaa agattatgtt atagaaggtg ggcttgaata
     3541 tgcaagagag ttgttaagca atgctttagg aaatgccaaa gcaaacaaat tacttgaagg
     3601 tatatcaata aatacaagca caaagccttt taaacatatt ataaaactag atgtaaagaa
     3661 aatattagaa atacttagga atgaaagtgc acaaactata gccgttttac ttgcacatat
     3721 ggagccagaa aatggagcag aagttttatc atacttagat tttgatttac aaaatgaagt
     3781 agctatgagg ataagctcaa tgggttcaat atctccagaa gtaataaaag taattgaaag
     3841 ttcattagaa gataaattac actcaatgag tggtgatgat tttaaggatt atggaggagt
     3901 gtctacctta gttcaaatat tgattaattc tgatagaaaa acagaaaaga atattttggg
     3961 aactattgaa gaacaagacc cggatttagc agctcaaatt aaatctcata tatttgtctt
     4021 tgaagatatt gtcaagttag atgatataag tatacaaaaa gtgttaaaag aagtgactat
     4081 gaaagattta gtatatgcct taaaaggtgt ggatgatgag atttcagatg ctatatatag
     4141 aaatcaatct tctagggcag cagaggcact tagagaagaa atggaaatgg ttggtgtcat
     4201 aaaagttaca caggttgagg ctgctcaaca aaagatagca aatatagtaa gaaaacttga
     4261 aagagaaggt gccataacta ttgtaaggaa ttttggtggt gactttgttg aataataaga
     4321 ttattaaaag taaaaaagca ttatataaag gagtgctaga tttaaccata atagataaag
     4381 ttagagtggg agaatcaaat gtaaataaag aaaatgattg tagatttagg gatgaggtaa
     4441 taaaagaagc tatagaatct gctgagataa aaaagaatga gatattagaa gaagcaaaaa
     4501 tgaatgcaca aaatatagaa aagcaagcat atgaaaaagg gtatagtcaa ggtcaaaaaa
     4561 atggatatga agatggatat aaagagtcat ataatgaata tacccaaaaa gctaaagatg
     4621 aagcagattt tataaaaaat caggcagact taatactatt acaagctaat gagaagatga
     4681 ctgagtacat aaaagaaaaa aataaagaga ttgtaagact tgctattaat atggctagtt
     4741 ctgttctaaa aaagcatttc gaagatgaag aatctatgag tgatttgtta aagcaaatta
     4801 ttcaagatta tgaaggaagt agttcattta tagtaagatg taattctttc tataaagata
     4861 atatagagaa agagtttgaa ttattcaaag aagaaacatc actaaaatca agtgtatttg
     4921 ttattgcaga tgatagtata gataaaggca atgctatcat ccaaaaggaa aatggaagaa
     4981 taatagttgg tatcgattgt gctatggaaa aactaaaaga agaattgctt ggtgaataaa
     5041 gtatgtataa aactgatata tctatagatt ttgacaaaat aaataagaaa atagaaaatt
     5101 tagaactcac tatatcagaa ggtagagtca aaaagataat aggacttact gtagaagttg
     5161 aaggaataaa agcttttgtt ggagagttat gtgttatata caatcaagtc aataaaccag
     5221 tgaattgtga agtagtgggt tttaaagata aagaagtcat attaatggcg ttgggagagt
     5281 taacattgat agctcctggt tgtaaagtta tttctaaggg gataccttta agtgttatgt
     5341 gtagcgataa tttactaggc aaagtacttg atggtcttgg aaatcctata gataactcag
     5401 atgctgtttt aggagataga tacaacttga acaatgaacc tcctgatcca atgaagagaa
     5461 aaaagatacg aaatataatg gaaacaggtg taagagcaat agatgcattt actacttgtg
     5521 gtgaaggaca gagaataggg atatttgcag gaagtggagt aggtaaaagt acgactctag
     5581 gtatgattgc taggaatgca aaagcagatg taaatgtaat tgcccttatt ggagaaagag
     5641 gaagagaagt attagatttt attgataaag atttaggtga agagggaatg aaaaaatctg
     5701 ttgttgtata tgcaacatca gataaagctc ctttggttag gctaaaaggt gctcttactg
     5761 ctactgcaat agcagagtat tttagagacc agggcaaaaa agtaatactt atgatggact
     5821 cggtaactag atttgctatg gcacaaagag aagttggtgt agcaatagga gagccaccag
     5881 ctcaaaaagg atatacacca tctgtgtttg ctattttacc aaaacttatg gagagaacag
     5941 ggacatcaga taaaggttct ataactgcat tttatacagt attagttgat ggagatgact
     6001 ttaatgagcc aatagcagat acaacaagag ggatacttga tggtcatata gttttatcta
     6061 gagacttggc aaataagaat cattatcctt ctatagatgt attgaacagt ttaagcagac
     6121 ttatgaatga gattgcttca aaagaagaca taaaaatagc atcttttgcg agagatatgc
     6181 ttgctgagta tagagaagca gaagatttga taaatatagg tgcatatgct tcaggtacaa
     6241 acaaaaaaat agatgaagca atctactatc atgagcatat tattaatttt ttaaaacaag
     6301 gaataaatga aaagagctct tttaatgaaa ctataagtag tttaagagga atctttgaat
     6361 aaacaattta aaaaaatatt tgaataggtt gtgatggaat tggataaaaa ttttaagttt
     6421 agattacaga aagttttaga cttaaaaata aaagatgaag aagagataaa aatggaattt
     6481 gctaagattc aacaaaaaaa aatagatata gaaactaatt tagaaaactt agaaagtaat
     6541 tacagcaaat attctatagg taaaaataat gatagtgttc aaaatcaaaa aataactatt
     6601 aactatttgc tagctttaaa caactctata atggatttat cagaaaaact ggataagtcg
     6661 acaaatgaac ttgaaaaagc tagaaaacag cttataagca aacagataga gagaaagtca
     6721 ttagaaaaat taaaagaaaa aaaatatgga cagtattata aagaagaaca gttaaaagaa
     6781 caaagcacta atgatgagtt tgcaagcatg agttatttga gaaatagaca agttctataa
     6841 aatagtagga agtgaggata ttattgtatg aagatacaag cagattctta taatattatt
     6901 aaagatattg ggaatagaaa aaactcatct aagaataaaa atataaaaaa tgataaattt
     6961 gaagataatc tatataagac aacagaccat aaaaaggtta aagatagaag agaaacaagt
     7021 aagcaaaatg ttagctcaga tagaccggaa aatagtgata atgagtgtca tttagatgaa
     7081 gataaaagta atcacttaga agatgaaaat acatatgaaa aagattgtag taataataat
     7141 gaagctttgc agcaattact gaatctttta aaagagaatt ataaaaaaga taaagatata
     7201 aacactatat tgaataatat aaaaaaacta agtatatcag atgaattaaa acaagaactt
     7261 aagcttaata aaaatatatt aaataagatt gcaaatcaaa attcagagct aaataaagct
     7321 aatataaata actctaatga tatagaaaat ttagacatag ttaaattaag agaacttttg
     7381 gaggataatg ctaataaaga tattgaagta attactaaaa ctacagaaaa tgatactaaa
     7441 aatatagatt tgtatagttt tgatagcaat agaatggata atcttaactc aaataaacag
     7501 aaagatgata gctctgatat tttggaaaaa ctagcagggg taaatggaaa taagagtggg
     7561 aattttggac aagtaataaa taaaaatgtt gatatgaata aaattaaaaa tgataatgtc
     7621 aatttaaaac agatagaatc aaatgtaatg gatgactcta taaaagctat taagcatatg
     7681 aaaacaaatg atatacaaga attgacaatt aaattaagac caaaagaatt aggagatatg
     7741 aatatacaat tgttaaaaga tggtgaaagt atgagagctg ttgtgactgt tttcaataaa
     7801 gatgtctttg actctataaa taaaaacata gcagatttaa agcaacatct tgaacttact
     7861 aatgtaaata taaaagatgt atcagtgcaa atgcacagcg ataatacaaa tacatctgat
     7921 acctttgata aagcatttga acaacagaat agacaaaata accaagaaaa catgagtaag
     7981 aagaatacaa atgataagca taataatgta gtcatagaag atgacattaa agaaaatact
     8041 attgatgatg atagagtaga tttactagct tagtaagggg gaagtattgc atgtacggaa
     8101 tagcaacggc tttggcagga actcagggaa actataggtc aaataactta agccaacttg
     8161 aaagaaatat taatgatatt gccaataaac aagttagaag caaaggaaat cagacagtaa
     8221 cagacaatgg gactccaatt ataggacaag gtgatgatgc tgataaagac ttgtttatga
     8281 agatacttgt ggcacagatg ggtaatcaag acccaatgaa tccgcaagac ccaacagaat
     8341 atgtgtcaca attagcacag tttgcaactt tagaaaagat gtcagctatg aatgagaagc
     8401 tagatgtact tttagcctta agtaattcca ctctaataaa ctctgcttta agtatggcaa
     8461 catcattaat tggtaaagaa gtagaagtat ctggggaaga gaaagaagat gaaaacattg
     8521 taggagtagt aaagtccaca tatataaaag atggggaagt acacttagaa ttagaagtag
     8581 atggaagtga caaaactgta ggtgttaaat atgaaaaatt acttaaaata ggagaggtag
     8641 atgaaaattc aggagaagaa aatgattctt cagaacacga atctccagaa gaagcataat
     8701 attaaatgag aaaagaggta aattcatgat aaaagcaatg tattcaggtg taagtggaat
     8761 gaaagcaaat caaacaaaat tagatgtaat aggtaacaat gtagccaacg ctggaactac
     8821 ttcttttaag aaaagttcag ctagaataac agattcattt tatcaaactg tactatatgc
     8881 aagtgctcca acagctgcat taggaggaac aaacttagga caagtaggtg taggtagtaa
     8941 gatatcaagt ataaataaag atatggtaca aggaaatgta caaccaacta atagaccatc
     9001 tgatttaatg atagatggag atggatatct tccagtagta agacaaggaa cagttatgta
     9061 tacgagagat ggttctttta atttagatac aggatttaaa gcagctgatg gtgctttgaa
     9121 gaatattaaa ggtggaagac ttgtaaatgg tgatggatat atgttacaag gtatgattta
     9181 tgatggggaa tataaagctg atactggaga atttgttaaa aagggtgatg ctaaaggaaa
     9241 agagcctatt gtaataccac ttcaaagtat aagtcctaag gatggaacag ttcaaaatgt
     9301 actagagtat aatatatcaa aggatggaac agtagaattt ttattgagtg atggtcaaag
     9361 aactcaattt gtctatgata tacctgatgg tgctaaggta ggagatacac ctgctggaat
     9421 agataaatta aaaggtacaa tacaaaaagt gcaaacatat gcattccaaa atccagcagg
     9481 actagatgca gaaggaggaa acttgtttaa accatcagca aactcaggag acccacaaat
     9541 agcaggagct acaggcacaa taatacaagg ggcaatagaa gcttccaatg tagatatagc
     9601 agaagaattt acagaaatga taatagcaag taggtctttc caagcaaaca gtaaaataat
     9661 atcgacatca gacgagatac ttcaagaaat aataaatctt aagagatagt aggtacatat
     9721 tatgataaga gtaacggatt taaacggaag aaaatacata ttaaattcag accttatagt
     9781 gaggattgat tctgtaccag aaagtaggat tctccttaca acaggagaaa aaaaccttgt
     9841 aaaagagtcg gtagatgaga tagtagataa agtaataaag tataaaagaa aaataaatgt
     9901 aggttatatt aggagcgaaa aataatgaaa aaaacagatt cactgacatt tattggacta
     9961 atagtatcta cagtattagt tttagttggt gccgcaaaag gaagtagttc agggttaaag
    10021 aatttttttg atgtttcatc aatattaatc acagtgcttg gttcatttgg agctctaatg
    10081 attacattta ctatagatga tataaaactt ataaaaaatg cattacaata ctcatttaag
    10141 acaatgagtg tatcaaagtt agatttacta gaacaattta aaacactttc taaaaaagct
    10201 agaaaagaag gtcttttatc aatagagagt gatgtaatgg gaatagaaga tccatttatg
    10261 aagaaagggt tagagctgtg tattgatgga ttggaaatca atgagataag gtccatctta
    10321 gaactagaga tagattctgt tgaagataca aataacagat actctaaact atttaaaact
    10381 tggggaacct ttgcacctgc ttttgggatg ttgggaacac taataggttt aatacaaatg
    10441 ttagctgacc ttacatctcc agatttaata gcatctggca tgggaaaggc attaattaca
    10501 acgttttatg gttctttact tgccaatatc gctttgaacc caattgcata taatatagat
    10561 gagaagactg aaaaagaaat ttatgtaaag gaaatgatgt tggaaggtat aataagcata
    10621 caatctgggg agagcagtat agtagttgag gaacgtttag ccacatacct ttcaaacaat
    10681 gagaagttag aaattatgaa atccaataaa aatacagaaa gagcaatgag caatggcgcg
    10741 taaaaaaaat aagaaaaagg atgagataaa tccagatgca tggctggcta cttatgcaga
    10801 tactataaca ctgattctta catttttcgt attattgtat gcaacatcag ttgtagatat
    10861 ggaaaaattt aaaaacatag cagatgcgtt aaaaggtcag ttttctggga caagtatatt
    10921 tagtacgggt tcaagtacaa gtgagaaatc tccaatagac agtgttttag tggataaaaa
    10981 tgataaatca aatttagaac aggatttaaa agataaagtt aataattcta acttacaaaa
    11041 tagtgtaaca gtcaaagatg atagtagagg gattttacta gagttagatg attcaatttt
    11101 atttgactct gggatttctg agttaaaagt agatagtaaa gctactctta ataaagtata
    11161 tgacatgata aagatgatga aaaataagat agttatagaa ggccatactg ataatgtacc
    11221 tgtaaaatca agtacacatg aatccaattg ggatttatct tctagtagag cagtaagtgt
    11281 tgtaagatac tttgtagaag aaaagggcat ggaccctaga ctattttcgg caacaggatg
    11341 tggagagtat caacctttgg tttcaaatga aacagcaaag ggaagggctg agaatagaag
    11401 agttaatata cttataacta ttgactagga ggaagaggtt tgttcaaaaa taaaaaaatt
    11461 attataatta ttttgatact gattattttc tcagcgggag caggtttttt tatattagga
    11521 gaaggtggaa ataaacaagt tgacaagatt gcaggtttgt ttattccaac aggaaaaggt
    11581 aaagatgaag taaagaccga aaatcttgac ttagaagaga tggtattaaa gttagctgat
    11641 gaaggtgtaa gatatttaaa agtatcaata actgtatctt atgattcttc atataaagat
    11701 atagaaaaaa atacgccttt aattagggat aatattataa attgttttat ggcaaaaagg
    11761 gcagatgatt ttactgctga taatttaaag aacataaaag aagaaattaa aaatacatta
    11821 aatatacaac taggtgaaaa tgtaattcaa aatatatatt tcacaaatat agtagtgcaa
    11881 taaaaggatg gtaagacatg atactttatt tactaaaaat attaatatgt atcccaataa
    11941 ttttaatatt gatagtctta tctttaaaaa ttagccaagg aactttatta aaaaaatata
    12001 atgataaata tgtaaaaata ctagatattg taaatataag taaaaacaat agcataataa
    12061 ttttaaaaat aggtgaagaa ggatgcatta tggcatcttc ttcttctagc atgagtaaaa
    12121 taaaagattt gaccaaagaa gaagtattaa aaatagaaaa cgacattaaa gaaagaaatt
    12181 ttaaaattca agattttaaa atggaaagaa ttaaaaagaa atatgatttc agtagatata
    12241 ttggaaaatt tacaaaaaag gaagataagt aaatggaact attatcaaaa ttacctttgg
    12301 ctgatgtacc atatacaagt tcaatgcaat tgtttttatt tctaactgca ttaatgtttt
    12361 taccatttgt aatgttcatg atgacaagct ttgtcagaat agttataagt ttatcatttt
    12421 tgaaatcagc attaggagca cagcaagcaa ttccaagtca aattttggta ggacttgcaa
    12481 tagtattgac aatatttata atgagaccag tattaaatga gataaacgaa aaggcattac
    12541 aaccatatat gaaagaagaa gttactatgg aagaagctat gaaggaagca gaaggaccta
    12601 ttaaagagtt tttattaaca caaaccagac agacagattt agatttattt gtagaacagg
    12661 ctggtttaaa agaaaagaaa ttaactcgtg aaaatatacc actatctgtt gtagttccag
    12721 catttgctat aagcgaacta aaaactgctt ttcaaatagg ttttttaata tatataccat
    12781 ttttgataat agaccttgtt gtggcaagtg ttttgatgtc tatgggtatg tttatgttgc
    12841 ctccagtcat gatatcatta ccatttaagt tattattatt tgtaatggtg gatggatgga
    12901 acttgatagt aaaaacattg atattgggat ttggatagga gaaaaatagt atgagtgaat
    12961 ttatgataat gagtgttctt aaaaatggca tgtttacagg agctatgatt gcagctccag
    13021 tactcattgt ttctcttgta gttgggcttt taataagtgt gtttcaagct acaactcaaa
    13081 tccaggagca aacattgact tttgtaccaa aactaatagc tattctttta gtagtagcta
    13141 tcggtggaag ctggatgtta catactctta ttagttttac ttctgagata ttcaatatga
    13201 tatctcttat aactaaataa gtaggataca tatgattttt gtatttatta gaatagtagc
    13261 attctttgga acactaagaa tactatttcc agctggtaca cctgctttat ttaagtcact
    13321 ctttgcaatc attatatcaa tattgatttc aagtactatg caaattgaat atgcaacaaa
    13381 tatagacaac attgtattgt ttaccttata tagtgtaaat gagaccataa ctgggattat
    13441 gcttggatat ataaccaacc tatgttttta tagtatcaga atggcgggtt ctatgatgga
    13501 ccaacagatg ggattatcaa tgattaatat gtttgaccca aattcaatga ctcagacaac
    13561 tttaattgat aatcttatga actggacagc acttatgata ttttttaaca tggatggtca
    13621 tcatgtttta attagaggta taaggtatag ttttgagtta attccaatag gaaaaccatt
    13681 tgtagataat aacatagatt atataataaa catatttgtg cagtgctttt taactggatt
    13741 caaaatagca atacctattg tactttgtct gcttatggca gattttattt tagggcttat
    13801 atcaagaagt gtacctcaat taaatgtaat gatagttggt atgccattaa aaatattggt
    13861 tggtattgca ttatttatca tatcaatacc tttaattgtc aatcaaataa gtcatttatt
    13921 aagtcaaata ccaaagatgt atgagggaac ttttgcacta gctcctatgt tttttatggg
    13981 ttcagcagat aaaacagaag aagctactcc aaagaaaaag ggagaacaaa gaaagaaagg
    14041 taatatagca aagagtagag aattacccgt tgcaatgact ctacttgcat tcaccttatt
    14101 agtacctaca ttattttcat atgtagtaga tacacttaaa tcttcgctta attatttttt
    14161 gagtctagat ttttatatga acataaatta ttcaaaccta gagaaattag ttatagcagg
    14221 attaatggat ttttttaaga tatttttacc aatagctatt ccctttttag ttttaggcat
    14281 aatagcaaac ctatttcagg tgggaatact gtttactggt gaaactttaa aaccaaattt
    14341 atcaaaatta aatccaatta gtggattcaa aaacatgttt tctatgagaa gtttgtcaac
    14401 cttaataaag gatacagcga ttatatctat tttggcttac ataggatata cattcttcca
    14461 agataattat ttagatatat taaagttagg taacatatac ctaccaactt taatgtacac
    14521 agttaaagac ttagtatatt caatattgag taagatttgt gtagctatga tagctatagc
    14581 agttgcagat tatgtttatc aaagatacag tcataaaaag caactgagaa tgacaaaaca
    14641 agaagttaaa gatgaatata aaaactcaga aggagaccca gaagtaaaag ctaagataaa
    14701 acaaaaacaa agacaaattt cgtctcaaag gacaatgcaa gcagtaccaa gtgctactgt
    14761 aatagtaacc aatccaaccc acttatctat agcagtgaga tatgaaaaag ggaaagacca
    14821 agctccagta gtggtggcaa aaggtgcaga ttatttagct tttaaaataa gggaaattgc
    14881 aaagggaaat gacataccta taatagaaaa taaacctatt gctaggttgt tatataagca
    14941 agtagaaata gaccaagaaa ttccagagga catgtatcaa gcttttgcag agatattagt
    15001 agcagtatat aaaatcaaaa atagatacaa agtccccaaa aggtaggata agtaatgaat
    15061 aagtttaatt taaaggaaaa tatggacgta atagtggcat ttggtgttgt agggattgtt
    15121 cttatgatga tagtgccgtt acctacattt atattagata ttttactggc aattaacata
    15181 agtttatctg tgctaatttt actaatgaca ctatttacaa caaatgtact tgatttatct
    15241 atatttccaa cagtgttact tgtgacaaca ctatttagat tgggattaaa tctttcatca
    15301 actagattga ttttaacaca gggatatgca ggtgaagtta tagaggcatt tggaagtttt
    15361 gttgtaggtg gaaattacgt agtaggaata attatattct taattatatt aattgttcaa
    15421 tttgttgtta taaccaatgg ttctggaaga gtatctgaag taacagctag atttacgtta
    15481 gatgccatgc caggtaagca gatgagtata gatgcagatt taaatgcagg agttatagat
    15541 gacaaaactg ctagaaaaag aagaaaagag cttcagcaag aagcagattt ttatggctct
    15601 atggatggag caaataagtt tgtaaaggga gatgccatag caggtcttat agtaacagca
    15661 ataaacataa taggtggaat tgtaatagga gctgtaatgc tgaatatgac tcttatgggt
    15721 gcagcacaga catatgtaag acttactata ggggatggtc ttgtaagcca gatacctgct
    15781 ctattaatat ctacatcagc aggtataatc gtaacaagag cttctactga tgaaaacttt
    15841 ggtggattag taggtaaaca gttaacagca ataccaaaag cagtaatgat gactggctgt
    15901 gttctaatcg tacttggtat gatgcctgga ttacctaaac cagcattttt ttcactaggt
    15961 ataggtgcta tagcagcagg ttacttttta ggaagagaaa agaaagatac tttagaagac
    16021 ttggagtttg aaaaacctga tatggtaaat ccagataaaa atgaagtaga agatgtaaca
    16081 aacttaataa atgtagaatc aatagaagta gaaattggat atggtttaat atctttagct
    16141 gatgaatcag caggaggaga tttacttcag agaatagcat caataagaag acaatgtgca
    16201 atagacatgg gtatagtcgt acaacctata agaataagag ataatttaca gttaaatcca
    16261 aaccaataca atataaaaat aaaagggaat gtagttggtg ggtatgagat aatgcctaat
    16321 atggttttat gtatggatcc tatgaatcaa ggatttagta tatttggaat aaagacaatt
    16381 gagcctactt ttggaataga ggcactttgg atagaaagtg acaaggtaga agatgcagag
    16441 ctaaagggtt atacagttgt agattcatcc acaatactta taactcactt actagatata
    16501 ataaaatcaa atgcacacga attactggga agacaagaag ttaagactat tatagatgca
    16561 gcaagagaaa attatagtgc tgtagtagat gaattgatac cagatttaat gacacttggt
    16621 gaaattcaaa aagtacttaa aaatctgtta agagaaaaag taaatataaa ggatagagtt
    16681 acaatacttg agacacttgc agaccagtct agaaatacaa aagatataga gcttcttact
    16741 gaatatgtaa gaatagcttt ggctagaagt atttgtaata atttagttga tgaagataag
    16801 gcaattgtag tggctacatt gtctccagaa actgaagaat tggtatctaa taatttacaa
    16861 aggtctgtaa atggaactta tccagcagtt gacccagaaa atacaaataa aatatttgag
    16921 agtatacaag aagtaatgaa caatgtatat ttcaatcata atataccagt aatagtagta
    16981 tcaccaaaga taagagctcc atttagaaaa ttagttgaaa ttgtatatcc taatttgact
    17041 gtattatcat taaatgaaat acctaacgat attaaaatca aagcagaagg tgtggtgagc
    17101 atatatgatg acgaaaaagt atacagctaa cacaattcaa gatgctatga atctagccaa
    17161 aatggaattg ggtgacaata ttacattaat agataaaaaa gaggtaagaa aatctggaat
    17221 aagaggcatt ttttcaaaga aagatattga attgactatt ggatgggaaa agaaagataa
    17281 tttagaacaa aaagatttaa aacgagagat agaacaacta aagtcaatta taaataatat
    17341 ggggtttgat aataaaaatg ataatgatat agataaaata tgcaagaatt tactaaactt
    17401 agatttaaat caagaaattg tagaatttat aaaagctgat ttacaagaaa tgaagttcaa
    17461 tggaattgat acaagtaaaa atttagtaga aagtctaaag aaaaagatta aaatagaaaa
    17521 tcaagcaata aatggtaaaa ttgctttagt tggaccacca ggagtaggta aaacgactac
    17581 aatagcaaag ttggctgcca aattagtttt tgaggaaaat aaaaaagtag gagtaataac
    17641 tatagataca tacaggattg gtgctgtaga acagttaaag atatatactg atataatgaa
    17701 tattcctttt aaaggggtaa taagccctga tgagatggaa ttggcacttg atgcgatgaa
    17761 agattgtgat gttgtattaa tagatacaac aggtagaggc tataaaaact ctatgcaaat
    17821 attagaaatc aaaaatctta tagataaagc tgaaactgat aatatacatt tagttttgaa
    17881 ctgtacaaca agagaaagtg atacaaaagc aattatagat tcatatagaa atgtgaattt
    17941 taaaagctta ataattacaa agttagatga aacaataaca tatggctcaa tatttaacat
    18001 aatgaattat gcacaaaaac caatatcata tattacaaca ggacaaaatg ttcctgatga
    18061 tattataaaa cctaatgaag ataagattat aagattacta ttaggagttg aaagtatatg
    18121 atagaccaag ctcaaatttt gagaaaaatg gcaatggaaa aaagaggttc tgatgagatt
    18181 atagtagaga atgagaatat gcctaagata attacaatag cttctggtaa aggcggcgtt
    18241 ggaaagagta atctcgctac aaatttgtct atatgcttaa ccaaacttga taaaaaagtt
    18301 cttatattag atgcagatat aggaatgtct aatatagata taataatggg ggtaaatgta
    18361 aaagggacta taattgatgt aattaatggc gaaaaaaata tagaagatat aatttcacag
    18421 actaagtatg gtgtaaatgt aatatctgga ggttctgccc ttaatcatat agaagacttt
    18481 acagaagctc aaagaaataa gtttatacat agcatagaac agattcacaa tgtggatttt
    18541 ataataattg atacaggagc tggtatgagc aaaagcctat tgtcatttat atactgtagt
    18601 actgaatttt tcttaataac aactccagaa ccgacatcat tgactgacgc atatagttta
    18661 ttaaaagcta taagtaattt tggcataaag aaaagtgcaa atataataat caatagggta
    18721 attgatttag aagaagcgaa atctacatac aaaagaataa aaatggttgt ggacaaattt
    18781 ctaaacctta atctggaatt gtatggatat ataatagatg acaaaaaagt aagtttatgt
    18841 gtaaaaaaac agataccatt cattttagaa tatcctacaa gtattgcatc aaaatgtatt
    18901 atagccatag caaagagaat ggtaagtcag tcaagagaag atattgttga taataaaagt
    18961 tttctaagaa agatgtttag tatatttaaa agctaaggag gcgtagttaa tgaatagaga
    19021 agaattaata aaagagaata tgcctcttgt aaagagtata gcaagaaaat tttttatacc
    19081 agggaaaggt tttgaatatc aagacttagt aaatagtgga gtcattggat tgattgatgc
    19141 aataaataaa tttgatgctg aaaaaggggc aaagttttct agttactctt atataaaaat
    19201 aaaatctgct atattagatg aaattagaaa tcaaagtcca atttctaaac ataatctaac
    19261 taaagtaaat aagtataata gagtggtaga aaagttgcaa tctaaattat taagagagcc
    19321 aacttcatat gaaattgcaa aagaattaaa ggtttcagaa aaagaacttc atgatatcga
    19381 aagtaatata gatatgttaa atattgtatc attaaactat gtaatctttg aagatacaaa
    19441 tgaaactgta caagatgtaa taagtgatag agaagaggaa gctccagaaa atataataga
    19501 agaagaagaa aagttagaga ttctatccaa agcaattagt aatttaaatg aaagagaaaa
    19561 attaatactt tcactgtact attatgaaga tttaaatcta aaggagatag ggaaagtgct
    19621 aggtgtttca gaatcaagag tttcacaact ccacagaaaa tcaataagaa acttaagaaa
    19681 caagataaaa gaacttaaat attctatata gatggtgatt gataagatga tacaaattat
    19741 attaatagta gtgtctattt taataatagt aactatagtt ataaatttac ataaggaatc
    19801 aaaaaataaa gataacagat actttgataa aatatttgag caagaatatg aagcaaatga
    19861 tagattaata attgcagaaa aaagaagatt ctttttagac aaagtacttg ctgaaataag
    19921 aagtaataat catataaaag tttatgaaaa ttataaccta ataaaggcta atgaagaaag
    19981 aaaggaaaac atatttatag aatcaaaaaa ggaagtaaga caagaaaatg agttatctct
    20041 taaagtgaaa catcttataa gttcaggtta tgatgatgtg gaaatatgta agatgctaga
    20101 tattggaaag ggggaactgt cattaataag gagttgttac aaaatataag gatattatta
    20161 tgtgataaaa aagtattctt aggaataggt attggtatat taatatcaac tttatgtata
    20221 atgcttttta gtaataatga tatgagtaag gcagagatag aaaaaaaggc cagagcgtta
    20281 ggcatgaaat atacaagtga gatgaaagtc ttagaataga tgatactgtt tagacattta
    20341 caaaaaagga ggtcataatg tgataagagg attatataca gcagtttctg ctatgataac
    20401 taatcaacaa aagcaaaatg ttgttgtcaa taacctagct aatatggata caaatggata
    20461 taaaagtaaa caacttttga ctaaaagttt tgatgaatta aagttggagg gttacaataa
    20521 ctacgcaaat ggaactaaac aaaagcagat tataggcgat ataagtccag gtatttctat
    20581 ggatgaaact atcacaaatt ttaatcaagg acctataaaa actactgata ataaaatgga
    20641 tgtagctatt caaggtaagg gattttttct agttagtgat gcagctggaa atcaatttta
    20701 cactagaaat gggaacttca gaacagacaa tcaaggtgac cttataacaa gtgaagggta
    20761 ttatgtactt gggacgaata ctgctactgg tgctgtaggt catataaatg taggaaatca
    20821 aaaatttgaa gtggcaaaag ataatacaat atcactagac ggaaatgcta tgtataaatt
    20881 taatattgta gattttaata actacaataa cttaaaaaaa gaaggagata acttatattc
    20941 tggaggagga gctatgaaag taaataatgc acttacaata caaagtgcga tagagtcctc
    21001 taatgtagat atggtatctg aaattaataa tatgatgaca atatcaagag aatatgaggc
    21061 aaatcaaaag ataattcagg ctatggattc taaattagca aaaatagcaa gtgagattgg
    21121 ttcagtaaga taggtaatag gaggtgaatt tttatgtaca caatgtttta tactagtaaa
    21181 acagctttat cagcaaatca aagtaaacta gatataatat ctaacaatat agcaaattct
    21241 gattcaacag gatataaaaa aataaatatg gaatattctg atttagtgga tgaagtacta
    21301 aatagaccat catatcctac aaatggcaat gatatatcga ctggaacagg agctaaggca
    21361 acagctccca tgagaaatta tgaacaaggt gcattggctc ctacagatag taagagtaat
    21421 ttggcaatag atggagaagg attctttaga gtgataagaa aagatggtac atatgcatat
    21481 acaagaaatg gtggatttaa tgtagatgct ttaggtaaga ttgtagatga taatggaaat
    21541 atattagatg tccaatttga tgcaggatat aactataata atacaaatat agatagtaca
    21601 aacctgacag tttcaagaaa tggagagcta ttcgtagata ataaaaaaat aggaaaaatc
    21661 aacttatatc aacctatagg aactcaaaat tttatatctg aaggagatag tttattcgtc
    21721 gcaactgatg cagcacagat taaacaagtt gagaaagtcg atatagttca aggctataga
    21781 gagaggtcaa atgtttcttt acaagaagaa tttgttgact taatagccac tcaaagagca
    21841 tttcaaatga actcaaaggg aataaagaca gcagatgaaa tgtggcaaat agcgaataat
    21901 ttaagagcta aataattatg taaggtgagg aatattcatg aatgatatga aagagtacga
    21961 ttttggcaaa cctcagagtt attcaagaga aaatttaact tgtttaaatt ttttattagc
    22021 tgaattttgt aaaaaatata aaaactatat aatttatgaa cttaagtgta attctagtat
    22081 ggttgttgat agtatagacc aagtaaatta tcagactttt ttagatagag taacttcttc
    22141 gtgcgtagta gtacaaaatg caatggaacc tgttatgaaa aacttttctt ttagaataga
    22201 tagaagtgta gctgatatgt ggattgatat aacttctgga ggaactggag ttgtaaagga
    22261 taatgataga gagcttacag aacttgataa aaaagtgctt ttacacctta tggatgattt
    22321 agtaagaaat atgcacttat ttgagggttt tgaaaatata caagtattgg atacacatac
    22381 acatattaat ttaccacaat tatgttctcc aactgcacca gtatgcgttg tagatttaaa
    22441 agtgctaaat gataatgagt atattggaag tgtttctctt tgttttccat acaatggttt
    22501 agaagctctg ttagaaagta tatctgttat agaatttttt gataatgata taggagatga
    22561 ttcagaggaa tttaccaaaa aaatatataa tagtgtatct aatatagaat tatcggttat
    22621 tgcagagata ggaaaagtat caattaatgt tgaagactta cttaaactag aagttggaga
    22681 tgtaatagta acaaataaaa aaataaatga ttatatagat atatttgtag aaaattcaaa
    22741 atcatataca gcaacaccag gatttatatc aagtaaaaaa ggtgtaaaaa taaatgatgc
    22801 tgttggaaaa gaggtgtagt tatggacaat agtaatctta tttcagatga agaagcaaag
    22861 atgctattaa atgataaagt tgaaagtgaa gttgatggag atgatgagtt agccttagat
    22921 gtgtctgaag ttaatcgaaa taatatacaa aggatacttg accttaagtt agaattatct
    22981 gtggttatag gaagaactaa gatgactcta agagatattt taaacctaca taaaggttct
    23041 cttattgaac tggatacttt agcagaccaa gatgtggaaa tactaataga taataaagtg
    23101 ttggcttatg gaaaagtagt ggtggtagat ttaaattttg gtgtaaagat aacaagtata
    23161 gtaagtgaag aagatatggt aaaatctctt gtttagagat aacatcaatt aatacttaag
    23221 gcatgaggtg gtacttattt taaacttaaa tgatataaaa aaagatatat taaaactcaa
    23281 taaaaaagac tcatgttctc tatataatga atctattaat aatataaata ataattgtat
    23341 atcgagtgga ttgaaatttt taaaacaagc aagagattta aacccagatg atgttgatat
    23401 attaaatctc acaggtcttg taaatttact aaaatgtaat tttgatgagg ctgtagaaag
    23461 tttctataag agttcacttt gtgaaaaaac tgctctgtgt gaaaagtata taaacatgct
    23521 tacatctgag gagtttttaa ttttctttga aaggtacaat caactcataa ggttaataaa
    23581 cagaggaatg tataaagaag ctatacaagg gtttaaactt ataactgagc aatactgtga
    23641 tttaatagaa ccttatgaat tgcttgcctt actgtatgac aaggaagaag aatatataaa
    23701 atttgatgaa tgtttagaag tattgaagat tattgataag gaaaattatt tattaaatca
    23761 agaaaataac acattagata tgtataatag tgctaatata caaactaaaa gtaatataaa
    23821 agtagatgat tataagaatg agttcttaca taaaagtagt agtaaccata aaattaatga
    23881 ttataatgat gtaactaaag aaaagagttc aagcaaaaac cagaaaagtt cagattctaa
    23941 taagaaaaaa agtattaaaa aaaataaata cctatatata gccataatat taggaatatt
    24001 attagtagga caaagtatat atagttctta taagatgaat aatttaactg ataaaatatc
    24061 taaaaataat gaaaccaaaa atgaagagtc aatagattct aacttgacta agaaagaaaa
    24121 taatatagaa gaaaatgaag atatgagtaa ggcttctaat ataaataaga gtaataaaaa
    24181 cattaataaa gaggatgatg taaaatctaa aaaaaatact gtagaaaatg aagatgataa
    24241 agattctgac ttatttacag aggatgaatt gatgtctaag gcgaatactt taaaatcaga
    24301 aaagaaaact aaatcttcta tacaactata taagaaagtt gcagatatag gtgaaaataa
    24361 agggaataca tctgaagcaa cttatcaagt tgctattcta agtgaaaagt taaaagacta
    24421 taaaactgct gaagaatatt ataaaatgta tgtagaaaat tactcagaaa aagatgctta
    24481 ttttgatgaa tcttattata atttaggtat gatgtactat aacaatggtg atttaaaaaa
    24541 ttctaaattg acattaaaga agttggttaa taaggtacca aatagtatgt ataataattc
    24601 aaaagtaaaa gaaattttaa aagaagaata gttaaatta
//
LOCUS       DAMJYC010000053        24526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJYC010000053 DAMJYC010000000
VERSION     DAMJYC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.054"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            154..2019
                     /locus_tag="PC472_002366"
     CDS             154..2019
                     /locus_tag="PC472_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2727685.1"
                     /translation="MNIPKKIISLLTITIMLSGLSLNTVNASTKESLVGSGRWETAIQ
                     ISKNGWENSKEAVLVNDSSIADALSATPFAKAKNAPILLTQSNSLDARTKAELKRLNV
                     SKVYLIGGNVTLSSNLEYELKSININSERISGDSRYSTSLELAKKIDSINTISKIVVV
                     NGQKGLADAVSVGAAAAQGNMPIILSDTTNGTSTSKDFISKHNITTSYVIGGTNSVSE
                     DIKNSLPNAKRISGENRNQTNAKVVEEFYKGTNLKNLYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVLLAGNKLDSVQKDILDTKIFDKITQVGGNGNEDVFKSIQDIQEETKYTV
                     ETISELDTAIKKSDANDIIRFKNDDKITGSIDISTKKAITIEFNGLYSGKVNINIPNG
                     DVYNYGDMTGQMDIKDIKTDTLVNEGSINEITIYDPNGCKIENEKDADIWKITLLDTS
                     KNTYINNSGTITKIVSDASNTTIKNSGKIELISGTKSPAVSGNQATDENIKDNDDKEA
                     GGISVYVDPCSPPKEGKVMLRIPKEPRSSSYNIYYRIVKSRPSAMDVGDTISKRGWTK
                     VTTRDSFMISATDGYYVEAVEIYDSDRKVSKWGRSGSIDDGYNNTTSSATLRSYK"
     gene            2328..2786
                     /locus_tag="PC472_002367"
     CDS             2328..2786
                     /locus_tag="PC472_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727686.1"
                     /translation="MNITNKCVLGISILAILSFGFSAFQGNAQITDRKKDYDIYLKAN
                     SDKSDVKSSLNDISKLEKKYGESDALSISKINLYEKMNDYKNLQSEIEKVYNRYPQTR
                     NTVSFVLLYSEVALKNGNKELSKNMINEAIKLGAQDAYKERVDKVLSQLN"
     gene            2799..4406
                     /locus_tag="PC472_002368"
     CDS             2799..4406
                     /locus_tag="PC472_002368"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDF2727687.1"
                     /translation="MLDKYYNRGKVEQIDKFILFILLFMMCIIPIITHEYTSTNYSPI
                     FTLTLYSSGERVEIFNFYKTAILYLGTMIVFCFFMYKIFVLKEELKKRKVNIILLILA
                     IGVILSSVFSDYKDIALFGNPDRFEGALAWFCYIVIFFVLYNIKIDVKDLKLFYFGLF
                     PFLVINLFLGLAQFFEIDVLQSKILTTLIGGGTLSGTFWTTLYHFNIMSGIASVIFCA
                     SLVYMLFEKDIKKKIVPLFGAIMSFTIILISNSISGFLTSILLFPIILVLSWRFVNKK
                     ELCVWTIIMVIISSIIYIILNRHNPNVYQETFAMFEKLNQASIFIVPILIVTFILLII
                     IMKFINKKKFFNFVTVCSIVIVSAGLLFFSYTLNKEVSILESNPNANITRIEDSKLFN
                     IINGMSTDRLNIWTKVMNFINERPILGNGFDTFPYKFISTDKNGALSTYGEVIDKPHS
                     WYMSVAYGSGIIGIIGLVGIIIYLTKEVFYSCVDKNNDKFLYIFFIGFLAYSIQGLFN
                     DSLAGSSIISWVFAGLTANRIFTIKSK"
     gene            4605..5564
                     /locus_tag="PC472_002369"
     CDS             4605..5564
                     /locus_tag="PC472_002369"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727688.1"
                     /translation="MNVSIIIPTKNAEKYIENLIKALINQTIEPCEIIVIDTESKDKT
                     VEICMEYDIVKFIQIKQSEFGHGSTRNRAAKLSKGDILVFMTQDALPQNCYFIEELIK
                     PLGKNNIVCTYGRQLPRNNAKTLEVFSRSFNYGNSDIIKSKEDIPKLGVKTFFFSNVC
                     SSFIKRDFFLVDGFPENIIMNEDMIISSKIIYNGKKTCYASKAIVTHSHRYTYLQQFK
                     RNFDIGVAFKENSYYLSEVKSESEGFKFVIEAIKFLFQSNKCYLIPSLFIESGFKFFG
                     YKFGMNYKNMPIKLVKLMSMHSFYFDRKEVMEEYYSENSNNRC"
     gene            5539..6390
                     /gene="rfbD"
                     /locus_tag="PC472_002370"
     CDS             5539..6390
                     /gene="rfbD"
                     /locus_tag="PC472_002370"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2727689.1"
                     /translation="MKIVITGAKGQLGLEIVNQLDNLNNIREKNIYDIVPTDRSNLNI
                     TDLENVNNFIFKEMPDVVINCAAYTKVDLCENDIDNAYKVNALGVRNLATACEKIKAK
                     FVQISTDYVFDGNSNIPYKEDCITFPNSIYGKSKLMGEYFTRQFSSKYFIIRTAWLYG
                     DGDNFVRTMLNLAKNNNEINVVDDQIGSPTSTVELAEAIINLIETDYYGIYHGTCEGE
                     CSWYEFANKIFELSKITVKVNPITSNEFKREAPRPMYSVLDNFMLKLIGLNNFNRWDE
                     ALKKYLE"
     gene            6418..7296
                     /gene="rfbA"
                     /locus_tag="PC472_002371"
     CDS             6418..7296
                     /gene="rfbA"
                     /locus_tag="PC472_002371"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2727690.1"
                     /translation="MKGIVLAGGSGTRLYPITKCVSKQVLPIYDKPMIYYPISVLMLS
                     GIRDILIISTVRDIKIFKELFGDGSQLGVNIQYAIQEKPNGIAEAFIIGEDFIGDDKV
                     ALVLGDNIFYGYGFSERLERAINRDEATIFGYHVSDPTSFGVVEFDKDFNVLSIEEKP
                     KIPKSNYAVPGLYFYDNEVIKIAKSIKPSKRGELEITSVNNEYLKKGKLKVELFGRGM
                     AWLDTGTHRGLLDASNYVEVVQTRQGLYIACLEEIAYIKGYITKEDLLELAKPLMKTD
                     YGKYLIKIANEFKENK"
     gene            7504..8400
                     /locus_tag="PC472_002372"
     CDS             7504..8400
                     /locus_tag="PC472_002372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727691.1"
                     /translation="MNSWRDTLYTPTSIEKIDDLYFIVDCWQHRVIYNDNLSDKISNW
                     KTLSDNLEGPHSIASDKEVFLVDNTEKNEIQVFKRKEDNFILTQTIQNVGKRPHKIIY
                     DKNTNLFYSIASTSQDIVVLENINGNVRVKQKTRLDFLDGSYIRSISIIEDKMYFLPG
                     PNKVICTDYKSGKFNVISEYKLPKALEAINHLYKIDNYYYISLYTMGENPNEPKLVRF
                     KNFNELQNNEYEDLTKKLNIKGVPYFFSEFDGKVFFTEIDSYSEIKSFKVNNDNIDSI
                     VNYYDSGKPSKESIERKKSILR"
     gene            8444..9016
                     /gene="rfbC"
                     /locus_tag="PC472_002373"
     CDS             8444..9016
                     /gene="rfbC"
                     /locus_tag="PC472_002373"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2727692.1"
                     /translation="MSKFNFIETKLKDLYIIEPTIFGDSRGYFMETYNKREFLEAGLD
                     MEFVQENESKSKKGVLRGLHFQTENVQGKLVRVAFGEVYDVAVDLRIGSPTYGLWEGV
                     ILSSENKRQFYVPEGFAHGFLVLSNEAVFNYMCTNYYNADYEGGILWNDKDINIEWPL
                     DKVDSVILSEKDKKLLKLSELDLQFKYGGE"
     gene            9020..10042
                     /gene="rfbB"
                     /locus_tag="PC472_002374"
     CDS             9020..10042
                     /gene="rfbB"
                     /locus_tag="PC472_002374"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2727693.1"
                     /translation="MSKVLITGGAGFIGGNFVHYILDKYPKYEVVNLDLLTYAGNLET
                     LENIKHKHNYKFVKGDISNRNFIFDLFDEERFDIVINFAAESHVDRSIKEPWKFINTN
                     VIGTQVLLDASNKYGVNRFHQISTDEVYGDLPLDRLDLLFTEKTNLNPSSPYSASKAS
                     ADLLTISYYKTYGLPVTISRCSNNYGPYHFPEKLIPLVIHRALENKELPIYGDGKNVR
                     DWLHVYDHCTAIDLIVHKGKSGNIYNVGGHNERSNLEVVKTILDTLGKSEDLISFVAD
                     RKGHDLRYAIDASKLEQELGWKSIYSFDEGIKHTIKWYLENQEWMESIISGEYKNYYS
                     NMYKNE"
     gene            10063..10851
                     /locus_tag="PC472_002375"
     CDS             10063..10851
                     /locus_tag="PC472_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2727694.1"
                     /translation="MSLVKELYRNRDLILNLSKNDFKTKYAGSYLGIFWAFVQPVVIV
                     LIYWFVFQVGFKAAPVKDFPFILWLIAGIVPWFFFQEALINSTNSLLEYSYLVKKVVF
                     KISILPLVKIISALYVHIFFICFALASYCIYGIYPNIYIIQILYYSFCTMFLALGISY
                     ITASIIPFFKDLSQIISIFLQIGMWATPIMWSYNIMPTKYQWFLKLNPMYYIVEGYRD
                     TLVNNVWFWERYNQTLYFWTISIIIFFVGIILFKKLKPHFSDVL"
     gene            10865..12133
                     /locus_tag="PC472_002376"
     CDS             10865..12133
                     /locus_tag="PC472_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2727695.1"
                     /translation="MNEIVIKVENISKVYKLYEKPIDRLKETIGLSKKVYHTKHYALE
                     NLSFQVEKGETIGIIGTNGSGKSTLLKMITGVLTPTSGNIIVNGKISALLELGAGFNP
                     EYTGIENIYLNGTMMGYKKEEMDDKIESILEFADIGEFINQPVKTYSSGMFSRLAFAV
                     AINVEPDILIVDEALSVGDVFFQAKCYKKFNDFKKQGRTIIFVSHDMGSIMKYCDKTL
                     LIDRGKFVDMGPSANMIDIYKKILVNQYVPDSDNEEIIEDVMEIEGTNKWYEKLQINP
                     TKLEYGNKKAEIIDFAIVDDKNKINNNINKGTIFNIKMKVKFNENIDNPIYAFTIKDL
                     KGTDVTGTNTMLEHIDTNKYDDKGEVIIVFKQKMDLQGGSYLLSLGCTGYESEDFTVY
                     NRLYDICNINVISLKNSVGYYDMNSEIEIY"
     gene            12161..13069
                     /locus_tag="PC472_002377"
     CDS             12161..13069
                     /locus_tag="PC472_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2727696.1"
                     /translation="MEKYIGNIKLNFDFYKGNDLYSDGVVEDELLDIVKNYSEYEDII
                     KERKEWPIIYHLSKIRENIIEWIPINKTDNVLEVGSGCGAITGILSKKAKQVDCIELS
                     QKRSYINAYRNKSKSNINIFVGNFEDIEKNMTEKYDYITLIGVFEYAESYINSKKPYI
                     EFLRIVKKHLKKNGKIIIAIENRLGLKYWAGCKEDHLGTYFEGLEGYREDKGIKTFSK
                     NELEDIFKLVGFYKYNFYYPYPDYKLPITIYSDEYLPKLGELNNNFRNFDLDRVVTFN
                     ETEVFDSIIKNNLFPIFSNSYLIILE"
     gene            13088..14470
                     /locus_tag="PC472_002378"
     CDS             13088..14470
                     /locus_tag="PC472_002378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727697.1"
                     /translation="MSKIIYSKYSNERNKKFQIRTSILQNDDGKKIVKKLAIVDESKK
                     HIDDIFENFERLKDNYYLDERVKINLCNKTEDGLEFEYLEGMTLDKIIDDFMENNEYD
                     KSIEIIKDFRNVIFNVSTTCGFNITEDFIKVFGNVDFNSDTKATLLSNIDSSFNNLVI
                     NDAWNIIDYEWVYKFPIPIRYILYRALNIYITTSVYGSKLKDLNIYDILGYSELELSK
                     YEFMEYNFGRYIAGESISLQNLYEDIKTKKYKLEELLISNDFNKRMQIFYDEGNGFSE
                     ENSYYIDLEMKNKITIPLEKDIKSIRIDPASVNCIILINKLDVVFANETIDIKNNLIT
                     NADEKKQDLYTFLSSDPQIYIDLGNRFQKGYINFEYEIKKLDFDTYDIYLEVRNLMLN
                     NQNKLINLNKELEERNMQLDILGKEINQINDRLENTLNELDNAKNKIYILEKRKIKNI
                     LRNMKKRISK"
     gene            14504..15664
                     /locus_tag="PC472_002379"
     CDS             14504..15664
                     /locus_tag="PC472_002379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727698.1"
                     /translation="MHFFTSITTNYLPKARVLANSVKKNCENSWFILVVCDNLPNDFN
                     LEKEPFDEVWTIESLGLPVENLNMWIFQHTVVELCTAVKGQAMLNLLNKYEKCVYLDP
                     DMVVFDSLKELENLLDSHDIILTPHVTHKEEEEQHIRNNEIASLRYGAYNLGFLAVKR
                     NENGLEFVTWWRDRLCKYCFDDIPGGLFTDQKWIDLAPGLFEGVYILRDPSYNVATWN
                     ITWRKISNLDGKYKVNNIDLKIYHFSGFDSGAQEIMLKVNGGNNQALWNLREQYILEQ
                     EKNGQKELGSLKGKFDTFDNGEIISKDMRVLMRNRLDLQDYFKEKNPFCSVGEKSYYN
                     WYKNNVSFRLDDETLDEKSKKILELENELNRLRKMISPLQQIKKILKKFLKR"
     gene            15675..19223
                     /locus_tag="PC472_002380"
     CDS             15675..19223
                     /locus_tag="PC472_002380"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF025665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HDF2727699.1"
                     /translation="MNNRDKNILFLIHDYIKYDTPMNVGGTQLHIEDLISKIENETFY
                     ILYKKKNKLILDEYRDSKLINTYNYYCGDLSSVVEYTNPLFKLIYSAILVNFKIDLIH
                     IHHLKNHTFDIIYEAKSLNLPIIITIHDYYLVCPKINLIDRNNCCCVNRETKELCGEC
                     MSEEIGMSFEELHLYREEVSKILLMCNEVIIPNKSVSEVLLIHYYCIKEKIRVIEHGQ
                     TGEVSKYAPTYKNGKFNVAFVGGLKPCKGSKEIIKIIKNCTDKDINFFVFGTIDDKDF
                     DDIPSHNINCTGNYFRKDIYKLLEENEIHIVCILSIWPETFCYTLSESWQCNIPVITV
                     DIGATGERVKNGGGILITGNNIGESTLKSIIELKNDRNKYESLCKTVKNIKLRDIGEM
                     SKDYSNLYFSYMKNVMLDNKISSELILDALKNIDLFQTNNSIKNYELEDLKNEIKYIK
                     GTFAYRVWQKYKDMKIPFKSNISSFFKRKVSLEKDISKNKNIIKRKSEQLNIISNIEY
                     EQVYYENEKLNFYVEGWFFSKYYDMNVSCENCESFSIERLSRPDVQSHFGGKKEAENS
                     GYRIAFIGVEENSKISLVFDIGNLKKYYNFNVDTSQGVVYIGKYPKKLRWKSIPQLFD
                     MTVVEPYKYKKVIDKNSETIENVSLILNATRPIKKEWAEETIRSIINQGFKSLYIISC
                     NENLNLFSEIDNIMLVSSKLLTRKEECLEFLTSKYDFNCEYVSFISIGDKMEVNTIRE
                     MIKEAKKSNSFIVTTNENRFFEEEYFAPFYKQQLYNRFDNPSIDRLCRKSMLISTDKI
                     IDFISGNIKSNEIKIIDKILYNYRLYNDNIDNKVKVLAYYLPQFHTIPENDEWWGEGF
                     TEWVNVNRGRPMFKGHYQPHVPGELGYYNLVKDEGIQKKQSQLANDYNIYGMCYYYYW
                     FNGKKLLEKPLENLIADKSINTNFCICWANENWCRRWDGLENEILIKQEHNVQSDKRF
                     IYDVLPILKDERYITIDNKPILLVYKSDLFPNFISTVESWNKICIENGLEGLEVITVQ
                     TGGLEDPRNMGCKYAVEFPPHNIYSRVLSAKSKVEELDKDFEGTILDYSEVAGIGMNK
                     KAQPYTFFRGVMLSWDNTARRNTQSRIFYGSNPNEYKKWIASVIDFSINFYEEKERMI
                     FINAWNEWAEGTHLEPDEKYGHKYLEVTKEVLGAQY"
     gene            19401..20771
                     /locus_tag="PC472_002381"
     CDS             19401..20771
                     /locus_tag="PC472_002381"
                     /inference="COORDINATES: protein motif:HMM:NF025665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HDF2727700.1"
                     /translation="MNKLNSLIKRLYHMLPLSYEKKILLKNNVYKLLSPFIKNTNMYS
                     IWINSLENPEKYIKDNNPKKGVDNEKDIYIDNLFKRASEKSSNYIEISDTRFQLEKND
                     IKLISFYLPQFHPIVENDEWWGRGFTEWTNVSKAVPQFLNHYQPHLPAELGFYDLRLK
                     EVMKRQIELAKMYGIYGFCFHHYWFAGKRLLERPVNMLLKDKELDIPFCLCWANENWT
                     RRWDGLENEVLIEQKHSKEDDLEFIKDISNYFKDERYIKIDGKPVLIIYRIELLPNPI
                     ETIKLWRKYCLEIGWNDIFIVGAQTFGFEEHIKYGMDAGVEFPPHNINNCPILNKEIN
                     FTNKKFTGLVYDYKKLVDDKLYIKKSHGKVFKTVMPSWDNTARKPNNASIFKNSTPEL
                     YKKWLKDIIYSTKENVLDSDNFIFINAWNEWGEGAHLEPDRKYGYSYLNATREAIIET
                     RSEKNN"
     gene            20812..22608
                     /locus_tag="PC472_002382"
     CDS             20812..22608
                     /locus_tag="PC472_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727701.1"
                     /translation="MDKLKKNNNLKIKIIFFILLQIFFIFFLVVNFKTPLLGEDYALL
                     YNGPFKDIGDYFSTIMAALEKQMSTWMVRMGNPLTIIWLSFDKSIYNIAVSFVSMIFF
                     FLIFFYGNARKPNVNNLGDLIGFFVPASLMMFLSPSIGDLFFWCNGSLNYLWPLCILL
                     LCAIPYRLYLSEKNINIKNPILIILYCIACFISGFTNENTPPVIITLVAALIIRDLYL
                     KKKPPKWIVFSFISICAGWIGLVVNSTTRFRSMYYRNMFGLPEHATINELINNVLRVG
                     YLFLKSGFAFVIIFLILSLLFIFILKSQKTENEFKYKHLFAENMFFILLTFISAAALV
                     FAPYTELRSFTLIHTIILATISNLLVNLYEISSHKQRLGLSSISIITLLSTFLLFINI
                     YNEYNDYYKFDTQRTCDIKSQVAAGKTTVYASKNFIENRTMNNREFYIYGNDLGQGGN
                     FYSDYFGAKKIIWYDKIDSNIDPATINLSSESTDVNVELINGNSEQDNSSLLSDGIFN
                     VSGWAIDKVAGNSPSKVFIKIDDKYYQARTVIREDVAKYFDNKKYKKAGFECSIPTKD
                     IKKGKHKVTLCVISNDGEKRYEVDSGKSIEFK"
     gene            22639..23529
                     /locus_tag="PC472_002383"
     CDS             22639..23529
                     /locus_tag="PC472_002383"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2727702.1"
                     /translation="MASLIKQRKYNRNILLEYIKLMRPKQYIKNGFVLAALIFSNNIL
                     NPSLALKSILSFIAFCMISSSAYILNDILDIEKDKMHPKKCKRPLASGSIGKKGAISL
                     GIVLVLSSILLSLLIHKNLCVIILLYLFNNIMYSLKLKNIILIDVFSIAIGFILRVCA
                     GSIAINVSLSSWIILCTFFLSLYLGFGKRKKEIILLKEDASNHRKILKEYDEENLNQM
                     MSISLSATIVCYSLYSANNIYNSNMIFTTVFVVYGVLRYNYIVNIGDEGNPTDVVLND
                     KSLKFCVLFWVIACIGILST"
     gene            23576..>24526
                     /locus_tag="PC472_002384"
     CDS             23576..>24526
                     /locus_tag="PC472_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727703.1"
                     /translation="MFMINIVIPNYNGYKLLKNCLDSLKNQTYSNFSIIVVDNASDDS
                     SYNWLNNYENVTLKKLDKNYGFSKAVNEGIKLSNSKYVVLLNNDTVVPENWLETLLGE
                     IEKDESIFSVCSKMIRYNEKDKIDDAGDEYSILGWAYKIGDGKNISSFRKSKYVFSSC
                     AGAAIYRNSIFDEIGYFDEDFFAYMEDIDISYRAKIYGYKNRYCSEAKIFHIGSATSG
                     SKYNSFKVRLAARNNVYVPYKNMPFLQLIINMPFLIIGFLVKYLFFLKKGFGNDYKEG
                     FFEGIKNLKNLNKTEFRIKNLFNYLIIEALLILNMCKYIFS"
BASE COUNT         9864 a         2239 c         4065 g         8358 t
ORIGIN      
        1 atctatgcta tgcaatatca aatattttaa tgttgtgtat gatgattcta gagtcttgaa
       61 atttttataa ttacaattta tattgacact taagttgttt aataagtaag agcattaaat
      121 gaaatatatt agaaattgag gggagtacaa gttatgaata taccaaaaaa aattatatcg
      181 ttattgacaa taacaataat gttaagcgga ttatctttaa atacagtaaa tgcttcaaca
      241 aaagaatctt tagttggtag tggaagatgg gagactgcta tacaaataag taaaaatggt
      301 tgggaaaatt caaaagaagc agtattagtc aatgatagtt caatagcaga tgcattatca
      361 gcaacaccat tcgcaaaagc taaaaatgct ccaattttat taacacaaag taatagtctg
      421 gatgcaagga ctaaagctga attaaaaaga ttaaatgtat caaaggtcta tttaatagga
      481 ggaaatgtta cactaagttc taatttagag tatgagttga aaagtataaa tattaactca
      541 gaaagaatat ctggagatag tagatattct acttcgttag aattagctaa aaagatagat
      601 agtataaaca ctatttctaa aattgttgtt gtaaatggtc aaaaaggttt agctgatgct
      661 gtaagtgttg gagctgcagc agctcaagga aatatgccta taatactttc agatactact
      721 aatggaacta gcacttcaaa agattttata agtaaacata atataacgac atcttatgta
      781 attggtggta ctaattctgt atctgaggac attaaaaatt cgcttcctaa tgctaaaaga
      841 atttctggag aaaatagaaa ccaaactaat gcaaaagttg tagaagaatt ttacaagggt
      901 acaaatttaa aaaatttata tataactaaa gatggtatga aaaaacaggg tgacttaatt
      961 gattcgctag ctgttggtgt tttagctgct aaaaatagtt cacctgttct tttggcagga
     1021 aataaattgg actcagttca aaaggatata ctagatacca aaatttttga taagattaca
     1081 caagttggtg gaaatggaaa tgaagatgtt tttaagtcaa tacaagatat tcaagaagaa
     1141 actaagtaca cagttgaaac aatttctgaa cttgatacag ctataaaaaa atcagatgct
     1201 aatgatatta taagatttaa aaatgatgat aaaataactg gctcaattga tataagtaca
     1261 aaaaaagcta taacaattga attcaatgga ttatatagtg gaaaagtaaa tataaatata
     1321 ccaaatggag atgtatataa ttatggagat atgactggcc agatggatat taaagatata
     1381 aaaactgaca ctttagtaaa tgaagggtca attaatgaga taactatata tgacccaaat
     1441 ggttgtaaaa tagaaaatga aaaagatgct gatatttgga aaataacatt actggataca
     1501 tctaagaata cctatataaa taatagtgga actataacta aaatagttag tgatgcatca
     1561 aatacaacta taaaaaattc aggaaagatt gagttaataa gtgggactaa gtctccagca
     1621 gtaagtggta atcaagccac tgatgaaaat ataaaagata atgatgacaa agaagcagga
     1681 ggaatttctg tatatgtaga tccatgttct cctccaaaag aaggaaaggt aatgttaaga
     1741 ataccaaaag aaccaagaag ttctagttat aacatatact acagaattgt taaatctaga
     1801 ccatctgcaa tggatgtagg agatactata tcaaaaaggg gttggactaa agtgactact
     1861 agagattcat ttatgattag tgcgacagat ggatattatg tagaagctgt agaaatctat
     1921 gattctgata gaaaggtaag taagtgggga agatcaggaa gtatagatga tggatataac
     1981 aatacaactt catcagcaac attaagaagt tataaataaa atattgatat gcatataaat
     2041 taagaaggat tttttgtggt tgttagtttc tatgtattta tgatataaat aatttaaaaa
     2101 tgagttatat aaacttgtta cattaattaa ttttaattat tgtgaagtta ttatatagct
     2161 tatttttatg aaaaagttta atgtacaatt taaggtgagt atactacttt tgatataaaa
     2221 cttttaaaat tattattcaa taaagtttta ataatttcaa aagtagtgta tactcatttt
     2281 atgaagaatt ttaatgaaat aagaataaat aaggaggaaa gagtaagttg aatataacta
     2341 ataaatgtgt tttaggaata tctattttag ctattttgag ttttggattt tcagcgtttc
     2401 aaggtaatgc tcaaattact gatagaaaaa aagattatga tatatatcta aaagcgaact
     2461 ctgataaaag tgatgtaaaa tcgtctttaa atgatatttc taagttagag aaaaaatatg
     2521 gagaaagtga tgcgttgagt atatctaaga ttaatttata tgaaaagatg aatgattata
     2581 aaaatttaca atctgaaatt gagaaagttt acaatagata tcctcaaact agaaatacag
     2641 tttcatttgt acttttatat tctgaagttg ctttaaaaaa tggaaacaaa gaattgtcaa
     2701 agaatatgat taatgaagca attaaattag gagcacaaga tgcttataaa gaaagagtgg
     2761 ataaggtatt atcacaatta aattaaaagg agaaatatat gttagataaa tattacaata
     2821 gaggaaaagt tgaacaaata gataaattta ttttgtttat attattattt atgatgtgta
     2881 ttattccaat aataactcat gaatatactt ctacaaacta tagtcctatt tttacattaa
     2941 cattgtattc ttcaggagaa agggtagaaa tatttaattt ttataaaaca gcaatattgt
     3001 atttggggac aatgatagtt ttttgttttt ttatgtataa aatttttgta cttaaagaag
     3061 aattaaaaaa aagaaaagtt aatataatat tgcttatctt agcaatagga gtaatattat
     3121 cgtctgtttt ttctgattat aaagatatcg ctttatttgg aaatcctgat agatttgaag
     3181 gagctttggc ttggttttgc tatatagtta tattttttgt gttgtataat attaagatag
     3241 atgtaaaaga tttgaaatta ttttattttg gattgtttcc ttttttagtt ataaatttat
     3301 tcttaggatt agctcaattt tttgaaattg atgttttaca atctaaaatt ttaactactc
     3361 ttattggtgg aggaacttta tctggaacat tttggacaac tttataccat tttaatatca
     3421 tgagtgggat agcatctgtc attttttgtg ctagcttagt atatatgtta tttgaaaaag
     3481 atattaagaa aaaaatagta ccattatttg gagcaattat gtcatttaca ataattctta
     3541 tatcgaattc tataagtggt tttttaacat ctatattatt attcccaatt atattagttt
     3601 tatcttggag atttgtgaat aaaaaagaat tatgtgtatg gacaattatt atggttataa
     3661 ttagttccat tatatatatt atattaaata gacataaccc aaatgtttat caagaaacat
     3721 ttgctatgtt tgaaaaatta aaccaagcat caatatttat agttcctata ttgatagtaa
     3781 catttatatt gttgattatt ataatgaaat ttataaataa aaagaaattt tttaattttg
     3841 ttactgtatg ttctattgtt atagtctcag ctggattatt atttttttca tatacactaa
     3901 ataaagaagt cagtatttta gaaagcaatc caaatgctaa tattactaga atagaagatt
     3961 ctaaattatt taatataata aatggtatga gtacagatcg tttaaacata tggactaaag
     4021 ttatgaattt tataaatgaa agacctatat tgggaaatgg atttgatact tttccatata
     4081 aattcattag tacagataaa aatggtgctt taagtacata tggagaggtt atagataaac
     4141 cacatagctg gtatatgagt gttgcatatg gaagtggtat aattggtata attggtttag
     4201 tgggtataat aatatattta actaaagaag ttttctatag ttgtgtggat aaaaataatg
     4261 ataaatttct atatatattt tttattggct ttttagcata ttctattcaa gggttattta
     4321 atgatagcct tgcaggatct tcaataataa gttgggtttt tgctggttta actgctaata
     4381 gaatatttac aataaagtct aaataaattt taaaaatggc atagtggact aattatagaa
     4441 aactatgtca ttttatttta aaaaaataga tattattttt agttagtatt ttaatctatc
     4501 tacaaataat ataaaataat agatgtatgt ttataattta aaaaaaatca aaatctaaag
     4561 taaaatgtgt agaatcatta aataaatcaa aaggggatga tagtttgaat gtgagtataa
     4621 taataccaac taagaatgca gaaaagtata tagagaatct tataaaagca ctaataaatc
     4681 aaactataga accttgtgag ataatagtta tagatactga atcgaaagat aaaactgtag
     4741 aaatttgtat ggaatacgat attgtcaaat ttatacaaat aaaacaaagt gaatttggtc
     4801 atggttcaac tagaaataga gctgctaaat taagtaaagg tgatattcta gtttttatga
     4861 cacaagatgc tttgccacaa aattgttatt ttatagagga actaataaaa cctttaggta
     4921 aaaataatat tgtatgtact tatggaagac aattgccaag aaataatgca aaaacactag
     4981 aagtattttc tagaagcttt aattatggaa atagtgatat aataaaatct aaagaagata
     5041 ttcctaaact tggagtaaag acttttttct ttagtaatgt ttgttcttct tttataaaaa
     5101 gagacttttt tttggttgat ggatttccag aaaatataat aatgaatgaa gatatgataa
     5161 tatcttctaa gattatatat aatggtaaaa aaacttgtta tgcatctaag gctattgtta
     5221 cacattcaca tagatataca tacttacaac aatttaaaag aaacttcgat ataggtgttg
     5281 catttaaaga aaatagttat tatttaagtg aagtaaagtc tgagtcagag gggtttaagt
     5341 ttgtcataga agccattaag tttttatttc aaagtaataa atgttatttg attcctagct
     5401 tgttcataga aagtggattt aaattttttg gatataaatt tggtatgaat tataaaaata
     5461 tgcctataaa attagttaaa ttgatgagca tgcattcttt ttattttgat agaaaagaag
     5521 ttatggagga gtattatagt gaaaatagta ataacaggtg ctaagggaca attaggttta
     5581 gagatagtaa atcaattgga taatttgaat aatataagag aaaaaaatat atatgatata
     5641 gttccaacag atagaagtaa cttaaatatt actgacctag aaaatgttaa taattttatt
     5701 tttaaagaga tgccagatgt cgttataaat tgtgctgctt ataccaaggt agatttatgt
     5761 gaaaatgata tagataatgc atacaaagta aatgccttag gtgttagaaa tttggctaca
     5821 gcctgtgaaa aaataaaagc taaatttgtt caaatatcta ctgattatgt atttgatgga
     5881 aatagcaata taccttataa ggaagactgt attacttttc caaattctat atatggaaag
     5941 agtaaattaa tgggtgagta ttttacaaga caattttctt caaaatattt tataattaga
     6001 acagcttggt tatatggaga tggtgataac tttgttagaa ccatgcttaa tttagcaaaa
     6061 aataataatg aaattaatgt tgttgatgat cagattggaa gtccaactag tactgttgaa
     6121 ttagctgaag ctataattaa tttgattgaa acagattatt atggaatata ccatggtacc
     6181 tgtgaaggtg aatgtagttg gtatgaattt gccaacaaaa tatttgagtt aagtaaaatt
     6241 actgttaaag ttaatccaat aacaagtaat gaattcaaaa gagaagcacc tagaccaatg
     6301 tattcagttt tagataattt tatgttaaaa ttaattggat taaataattt taatagatgg
     6361 gatgaagctt taaaaaagta tttagaataa aaaataaatt tgtttggagg ataaattatg
     6421 aaaggaattg tgctagcagg cggatctggg actaggttat atcctataac taaatgtgtg
     6481 tctaaacaag tactaccaat atatgataag ccaatgattt attatcccat atcagtgctt
     6541 atgctttcag gtataagaga tatactaata atttctactg tcagagatat aaaaattttt
     6601 aaagaattgt ttggagatgg atctcaatta ggagttaata tacaatacgc tattcaagaa
     6661 aaaccaaatg gtattgcaga agcatttatt ataggagagg acttcattgg agatgataaa
     6721 gtagctcttg ttttaggaga taatatattt tatggttatg gatttagtga acgattagag
     6781 agagctatta atagagatga agctactata tttggatatc atgtaagtga cccgacatct
     6841 tttggtgtag tagaatttga caaagatttt aatgtactat caatagagga gaaacctaaa
     6901 atacctaagt ctaactatgc tgttccaggt ctttactttt atgataatga agttataaag
     6961 attgctaaga gtataaaacc ttctaaaaga ggagaattag aaataacttc tgtaaataat
     7021 gagtatttaa aaaagggaaa gcttaaagta gaactttttg gacgtggtat ggcttggcta
     7081 gatactggca cacatagagg gctattagat gcatctaatt atgtagaagt tgttcaaaca
     7141 agacaagggt tatatattgc ttgtttagaa gaaattgctt atattaaggg atatataaca
     7201 aaagaagatt tactagaact tgctaagcca ttaatgaaaa ccgattatgg taaatatttg
     7261 ataaaaatag ctaatgaatt taaagaaaat aaatgaaaat aagataatta ttaaaactgt
     7321 atgaatacta taatataaaa atgacaactt agtttatata atatttgatt gtataaatta
     7381 agttaaatta tgtactaaat atttaaacaa taagaaggtg taattttgaa tattaaaaaa
     7441 aaatttaaaa tttgtattct acttatactt gtatgtatgt tatttttagg agcttggtct
     7501 aaaatgaatt cttggagaga cacattatat acacctacat caatagaaaa aatagatgat
     7561 ttatatttca tagtagattg ttggcaacac cgtgttatat ataatgacaa tcttagtgac
     7621 aaaatatcta actggaagac tttaagtgac aatttagaag gtcctcattc tattgcaagt
     7681 gataaagaag tatttctggt agataataca gaaaagaatg aaatccaagt ttttaaaaga
     7741 aaagaagata attttatatt aactcaaact attcaaaatg ttgggaaaag accacataaa
     7801 atcatatatg ataagaatac aaatttattt tacagtattg ctagtacctc acaagatata
     7861 gtagttcttg aaaatatcaa tgggaatgta agagttaaac aaaaaactag gttagatttt
     7921 cttgatggat cttacataag gtcaatttct attattgaag ataaaatgta ttttttacca
     7981 ggtcctaata aggtgatttg tacagactat aaaagtggaa aatttaatgt gatttctgaa
     8041 tataagcttc caaaagcttt agaagcaata aatcatcttt ataaaataga taattattat
     8101 tacatatcac tttacacaat gggtgaaaat cctaatgaac ctaaattagt tagatttaaa
     8161 aattttaatg aacttcaaaa taatgaatat gaagatctta caaaaaaact aaatattaaa
     8221 ggagtacctt actttttttc agagtttgat gggaaagtat tttttacaga aatagactct
     8281 tattcagaga ttaaatcatt taaagtaaat aatgataata tagatagtat tgtgaattat
     8341 tatgattcag gcaaaccatc aaaagaatct attgaaagaa agaaaagtat tttaagatag
     8401 atatcaatga ttaaatatta ttgtataata aaggagaaat actatgagta agtttaattt
     8461 tatagaaaca aaattaaaag atttgtatat aatagaacct acaatatttg gagatagtag
     8521 aggatatttt atggaaacct acaataaaag agagttttta gaggctggac ttgatatgga
     8581 gtttgttcag gaaaatgaat ctaaatctaa aaaaggtgtt ttaagaggat tacattttca
     8641 aactgaaaat gttcaaggaa aactagttag agtagctttt ggagaagtgt atgatgtagc
     8701 tgttgattta agaattggtt ctccaactta tggactatgg gaaggtgtta tactatcatc
     8761 agaaaataaa agacaatttt atgtaccaga aggttttgca catggatttt tagtactatc
     8821 taacgaagct gtctttaatt atatgtgtac taattattat aatgctgatt atgaaggtgg
     8881 catattatgg aatgataaag atataaatat agaatggcct ttagataaag tagacagcgt
     8941 tatattatct gaaaaagata aaaaactatt aaagttaagt gaattagatt tacaatttaa
     9001 atatggaggt gagtagaaaa tgagtaaagt tttaattact ggaggcgcgg gatttattgg
     9061 tggaaatttt gttcattata tcttagataa atatcctaaa tatgaagttg taaatttgga
     9121 tttgttaact tatgctggta acttagaaac attagaaaat ataaaacata aacataatta
     9181 taaatttgta aagggtgata tttcaaatag gaattttata tttgatttat ttgatgaaga
     9241 aagatttgat atagtcataa attttgcggc tgaatcacat gtggataggt ctatcaaaga
     9301 accgtggaaa ttcattaata caaatgttat tggaacacaa gtactgctag atgcttctaa
     9361 taaatatggt gtaaatagat ttcatcaaat ttccactgat gaagtttatg gagatttgcc
     9421 acttgataga ttggatttac tttttactga aaaaactaac ttaaatccaa gttcaccata
     9481 ttctgcttct aaagcatcag cagatttatt gactatttca tattataaga catatggatt
     9541 acctgttact atatcaagat gttcaaataa ttatggacca tatcattttc ctgaaaaact
     9601 aatacctctc gtaattcata gagcattaga gaataaagag ttacctattt atggagatgg
     9661 aaaaaatgta agagactggt tgcatgtata tgatcattgt acagcaattg atttaatagt
     9721 tcataaaggt aaaagtggaa atatttataa tgtaggtgga cacaatgaaa ggtctaattt
     9781 agaagtagtt aaaactattt tagatacttt aggtaaatct gaagatttaa tatctttcgt
     9841 tgcagataga aaagggcatg atttaagata tgctatagat gcaagtaaat tagagcaaga
     9901 attaggatgg aaaagtatat atagttttga tgaaggtata aaacatacaa taaagtggta
     9961 tttagaaaat caggaatgga tggaaagcat aatatctgga gagtataaaa attattatag
    10021 taatatgtat aaaaacgaat aatattgatt ggagaaatta aaatgtcatt agtaaaagaa
    10081 ttatatagga atagagacct gatattaaac ttatctaaaa atgattttaa aactaagtat
    10141 gcaggttcat atcttggaat attttgggca tttgtacaac cagtagttat agttcttata
    10201 tattggtttg tttttcaagt tggttttaaa gcggcacctg taaaagattt tcctttcata
    10261 ttatggttaa ttgcaggaat agtcccttgg ttcttttttc aagaagcact cataaattcc
    10321 acaaatagtt tgcttgagta tagttatttg gttaaaaaag ttgtatttaa aataagtata
    10381 ctaccattag taaaaatcat atctgcttta tatgtacaca tattttttat ttgttttgca
    10441 ctagcttctt attgcatata tggtatatat ccaaatatct atataattca aatattatat
    10501 tattcttttt gtactatgtt tttagctctt gggatatcat acataacagc ttctataatt
    10561 ccttttttta aagatttaag tcaaattata agtatatttt tacaaattgg aatgtgggct
    10621 acaccaataa tgtggagtta taatataatg ccaacaaaat atcaatggtt tttaaaatta
    10681 aatcctatgt attatattgt ggaaggttat agagatacat tagtaaataa tgtttggttt
    10741 tgggaacgtt ataatcaaac attatacttc tggactatat ctataatcat attttttgtt
    10801 ggaataatat tatttaagaa attaaaaccg catttttcag atgtgctgta gaagaggtat
    10861 acatatgaat gagattgtta taaaagttga aaatataagt aaagtgtata aattatatga
    10921 aaagccaata gatagactaa aagagactat agggttatca aaaaaagtat atcatacaaa
    10981 gcattatgca ttagaaaatc tttcgtttca agttgaaaaa ggagaaacta taggcatcat
    11041 aggtacaaat ggttccggaa aatctacttt attaaagatg ataacaggag tattaactcc
    11101 aacaagtggt aatataattg tcaatgggaa gatatcagcc ttattggaat taggagctgg
    11161 atttaatcct gaatatacag gtatagaaaa tatctactta aatggtacaa tgatgggata
    11221 taaaaaagaa gaaatggatg ataaaataga atcaatacta gaatttgcgg atattggtga
    11281 atttattaat caacctgtta aaacttattc aagtggtatg ttttctagat tggcttttgc
    11341 agtagcaatt aatgttgaac cagatatact tatagtagat gaagcactta gcgtaggaga
    11401 tgtttttttt caagcaaaat gctataaaaa atttaatgat ttcaaaaaac agggtagaac
    11461 tattatattt gtaagtcatg atatgggaag tataatgaaa tattgtgaca agacattgct
    11521 tattgatagg ggcaaatttg ttgatatggg cccatcagcg aatatgattg atatatataa
    11581 aaaaatatta gtgaatcagt atgttcctga tagtgataat gaagaaatta ttgaagatgt
    11641 tatggagatt gaaggtacaa ataaatggta tgaaaaatta caaatcaatc ctacaaaact
    11701 tgaatatgga aataaaaaag cagaaataat tgattttgct attgtagatg ataaaaataa
    11761 aattaataat aatataaata aaggtacaat atttaatata aaaatgaaag ttaaatttaa
    11821 tgaaaatata gataatccaa tttatgcatt cacaataaag gatttgaaag gtactgatgt
    11881 aacaggaaca aatacaatgt tggaacatat tgatacaaat aaatatgacg ataagggtga
    11941 agttataata gtatttaagc aaaaaatgga tttacaagga ggttcatatt tattatcatt
    12001 aggatgcact ggatatgaaa gtgaagattt tacagtatat aacaggttat atgatatatg
    12061 taatataaat gtaatatcat taaaaaatag tgttgggtac tacgatatga attctgaaat
    12121 cgaaatttat taagttataa ttgaaaggta gttttagaat atggaaaaat atataggtaa
    12181 tataaaatta aattttgatt tttataaagg aaatgattta tattctgatg gagtagttga
    12241 agatgaactt ttggatattg taaaaaatta tagtgaatat gaagatatta taaaagaaag
    12301 aaaagaatgg ccaattatat atcatttatc taaaataaga gaaaatatta ttgaatggat
    12361 tcctatcaat aaaacagata atgttttaga agtaggttca gggtgtggtg caattacagg
    12421 aatattatca aaaaaagcaa aacaagttga ttgtatagaa ttatctcaaa aaagaagtta
    12481 tatcaatgca tataggaata aaagtaaaag taatataaat atatttgttg gaaattttga
    12541 agatatagaa aaaaatatga ctgaaaagta tgattatata actttaatag gagtatttga
    12601 gtatgcagag agttatatta attctaaaaa accatatatt gaatttctta gaatagtaaa
    12661 gaagcatcta aagaaaaatg gtaaaattat tattgcaata gaaaataggt taggattaaa
    12721 atattgggct ggatgcaaag aagatcattt aggaacatat tttgaaggtt tagaaggata
    12781 tagagaagat aaaggtataa aaaccttctc taaaaatgaa cttgaagata tatttaagtt
    12841 agttggtttt tataagtaca acttttatta tccatatcca gactacaaat taccaataac
    12901 aatctattca gatgaatatc tacctaaatt aggagaactt aataataatt ttagaaattt
    12961 tgatttagat agagtagtta catttaatga aactgaagta tttgattcta ttattaagaa
    13021 taacttattt cctatttttt caaattctta tttaataata ttagaataaa aggaagtgaa
    13081 aatccagatg agtaaaatta tttacagtaa atattcaaat gaaagaaata aaaaatttca
    13141 aataaggaca agtatattac aaaatgatga tggaaaaaaa attgtaaaaa aattggctat
    13201 tgtagatgaa tctaaaaaac atattgatga tatttttgag aactttgaga gattaaagga
    13261 taattattat ctggatgaaa gagtaaaaat aaatctatgt aataagactg aggatggttt
    13321 agagtttgag tatttggaag gtatgacatt agacaaaata atagatgatt ttatggaaaa
    13381 taatgaatat gataagtcta tagaaattat taaagatttt agaaatgtca tttttaatgt
    13441 gagtactact tgtggtttta atattactga agattttata aaagtatttg gtaatgtaga
    13501 tttcaattct gacacaaagg ccacactatt aagcaacata gattcttcct ttaataatct
    13561 tgttattaat gatgcttgga atataattga ttatgaatgg gtatataaat tccctatacc
    13621 aataagatat attttataca gagctttaaa tatttatata actacatcag tttatggaag
    13681 taagctaaaa gatttaaata tttatgacat attaggatat agtgagttag aattatctaa
    13741 gtatgagttt atggaatata attttggacg ttatattgct ggtgaatcta tatcattaca
    13801 aaatttatat gaagatataa aaacaaaaaa gtataagtta gaagagttat tgattagtaa
    13861 tgattttaat aaaaggatgc aaatattcta tgatgaaggc aatggcttta gtgaagaaaa
    13921 ctcatattac attgatttag aaatgaagaa taaaattaca attccattag aaaaagatat
    13981 taaatctatt agaatagacc ctgcttctgt taattgtatt attcttataa ataaattgga
    14041 tgtagttttt gcaaatgaaa ctatagatat aaaaaataat ttaattacta atgcagatga
    14101 aaaaaaacag gatttatata cttttcttag cagtgaccct caaatatata tagacttagg
    14161 gaataggttt caaaaaggct atataaactt tgaatatgag attaaaaaac ttgattttga
    14221 tacatatgat atttatttag aggtcagaaa tttaatgtta aataatcaaa ataagttaat
    14281 aaatctaaac aaagaacttg aagaaagaaa tatgcaatta gatatactag gtaaagaaat
    14341 caatcaaata aatgatagat tagaaaatac actaaatgaa ctggataatg caaaaaataa
    14401 aatttatata ttagagaaaa gaaaaataaa gaatatattg agaaatatga aaaaaagaat
    14461 tagtaaataa ttattttatt tcaaaagaag gagtattaaa aatatgcatt tttttacaag
    14521 tattactaca aactatttgc caaaagctag agtattagca aattctgtaa agaagaattg
    14581 tgaaaactca tggtttattt tagttgtatg tgataatcta ccaaatgatt ttaatttgga
    14641 gaaagaacct tttgatgagg tttggactat tgagagttta ggactacctg ttgaaaattt
    14701 aaatatgtgg atttttcaac atacagtagt agaattgtgt actgccgtca aagggcaagc
    14761 catgcttaat ttattaaata aatacgaaaa gtgtgtgtat ttagatcctg atatggtagt
    14821 atttgatagt ttgaaggaac ttgaaaatct tttagacagt catgatataa tattaacacc
    14881 acatgtaact cataaagaag aagaagaaca acatattcgt aataatgaaa ttgcatcact
    14941 acgttatggt gcatataatt taggattttt ggctgtaaaa agaaatgaaa atggtttaga
    15001 gtttgttaca tggtggagag atagactttg taaatattgc tttgatgata taccaggagg
    15061 actttttact gaccaaaaat ggatagatct tgcaccaggg ctatttgagg gggtatatat
    15121 attacgagat ccttcatata atgtagctac ttggaatatt acatggagaa aaatcagtaa
    15181 tttggatgga aaatacaaag ttaataacat tgatttaaaa atatatcact tttcaggttt
    15241 tgattctgga gcacaagaga taatgttgaa agtaaatgga ggaaataatc aagcgttatg
    15301 gaatctaaga gaacagtata ttttagaaca agaaaagaat ggtcaaaaag agttaggaag
    15361 tttaaaaggt aaatttgata catttgataa tggagaaatc atatcaaaag atatgagagt
    15421 attgatgaga aatagattag atttacaaga ttattttaag gaaaagaatc cattctgctc
    15481 tgttggagaa aaaagttatt ataattggta taaaaataat gtatctttta ggcttgatga
    15541 tgaaacttta gatgaaaaaa gtaaaaaaat tttagaactt gaaaatgaac taaatcggct
    15601 aagaaaaatg ataagcccac tacaacaaat aaaaaaaatc ttgaaaaaat ttttaaagag
    15661 ataggaggaa aatcttgaat aatagagata aaaatatttt gtttttgata catgattaca
    15721 taaaatatga tactcctatg aatgtaggcg gaacccagtt acatatagaa gatttaattt
    15781 caaaaataga aaatgagaca ttttatattt tatataagaa aaaaaataaa ttaatcttag
    15841 atgaatatag agattcaaag ttaataaata catataatta ttattgtgga gatttatctt
    15901 cagtagtaga atacacaaat ccgttattta aacttatata ctctgctata cttgtcaact
    15961 tcaaaataga tttgattcat atacatcatt taaaaaatca cacatttgat ataatatatg
    16021 aagctaaaag tttaaactta ccaattatta ttactataca tgattattat ttagtatgcc
    16081 ctaagattaa tctgattgat agaaataatt gttgttgcgt aaatagagag actaaagaac
    16141 tctgtggaga atgtatgtct gaagaaatag gtatgtcatt tgaagaacta catttgtata
    16201 gagaggaagt aagtaaaata ttattgatgt gcaatgaggt tattatacca aataaatctg
    16261 ttagtgaagt acttttaatt cactattatt gcataaaaga aaagataaga gtaatagaac
    16321 atggacaaac aggtgaagta tctaaatacg cacctacata taaaaatgga aagtttaatg
    16381 ttgcatttgt aggaggattg aaaccatgca aaggtagtaa agaaattata aaaataatta
    16441 aaaattgtac tgataaagat attaattttt ttgtatttgg gacaatagat gataaagatt
    16501 ttgatgatat tccaagtcat aatataaatt gtacaggaaa ctattttaga aaagatatat
    16561 ataaattatt agaagaaaat gaaatacaca ttgtttgtat attatcaatt tggcctgaaa
    16621 ctttttgtta cactttaagt gaatcttggc aatgtaatat acctgtgatt acagttgata
    16681 ttggagctac aggagagaga gtgaaaaatg gtggaggtat tttaattact ggaaataata
    16741 ttggagaaag tactctaaaa tcaattatag agttaaagaa tgatagaaat aaatatgaaa
    16801 gtttgtgtaa gacagttaaa aacataaaac tacgagatat tggagaaatg tctaaagatt
    16861 attctaacct atattttagt tatatgaaaa atgttatgtt agataataaa atttcttcag
    16921 agctaatatt agatgcttta aaaaatatag atttatttca aacaaataac agtattaaaa
    16981 attatgaact tgaagatcta aaaaatgaaa ttaagtatat aaaaggtact tttgcctatc
    17041 gtgtttggca aaaatacaag gatatgaaga taccttttaa gagtaatata tctagtttct
    17101 ttaaaagaaa agtatcttta gaaaaagata tatcaaaaaa taaaaatatt ataaaaagaa
    17161 agtctgaaca attgaatata atatcaaata ttgaatatga acaagtttat tatgaaaatg
    17221 aaaagttaaa cttttatgtt gaaggttggt ttttcagtaa atattatgat atgaatgtat
    17281 cttgcgaaaa ttgtgaaagt tttagtatag aaaggttaag tagaccagat gtacaatctc
    17341 attttggtgg gaaaaaagaa gctgaaaata gtggatatag aattgctttt ataggggtag
    17401 aggaaaatag caaaatatca ctggtttttg atataggtaa tttgaagaaa tattataatt
    17461 ttaatgttga tacttcacag ggagttgtat atattggaaa atatcctaaa aaacttagat
    17521 ggaagagtat accacaatta tttgatatga cagtagtaga gccttataag tataaaaagg
    17581 taatagataa aaatagtgaa acaattgaaa atgtttctct gatattaaat gcaactcgtc
    17641 caataaaaaa agagtgggct gaagaaacca ttagaagtat aataaatcaa ggatttaaat
    17701 ccttatatat aatttcttgt aatgaaaatt taaacttatt ttcagaaata gataatatta
    17761 tgctagtgag ttctaaattg ttaaccagaa aagaagagtg tttggagttc ttaactagta
    17821 aatatgattt taattgtgag tatgtttctt ttatatctat tggggataag atggaagtta
    17881 atacaattag agaaatgata aaggaagcaa aaaaaagtaa tagttttata gtaactacaa
    17941 atgaaaatag attttttgaa gaagaatatt ttgctccatt ttacaaacaa caactttata
    18001 atagatttga caatccatca atagatagat tatgtagaaa gagtatgtta atttctactg
    18061 ataaaataat tgattttatt agtggaaata taaaaagtaa tgagattaag attattgaca
    18121 agatattgta taattataga ttatataatg ataacataga taataaagta aaagtattgg
    18181 catattattt accacaattt catactattc ctgaaaatga tgagtggtgg ggagagggat
    18241 ttacagaatg ggtaaatgta aatcgtggac gacctatgtt caaaggtcat tatcaaccac
    18301 atgttccagg ggagttaggc tattacaatt tagtaaaaga tgaaggaatt cagaagaagc
    18361 aatctcagct agcaaatgat tataatatat atggaatgtg ctattattac tattggttta
    18421 atggtaaaaa attattggag aaaccgttag aaaatcttat agctgataag agtataaata
    18481 caaatttttg tatttgttgg gcaaatgaaa attggtgtag aagatgggat ggacttgaaa
    18541 atgaaatact tataaaacaa gaacataatg ttcagagtga taaaagattt atttatgatg
    18601 tattaccgat actaaaggat gaacgttata taactataga taacaaaccg attttactag
    18661 tttataagtc agacttattc ccaaatttta tatcaactgt tgaaagttgg aataaaattt
    18721 gtatagagaa tggtctagag ggtctagaag taattactgt tcaaactgga ggtctagagg
    18781 atccaagaaa catgggctgt aaatatgcag ttgaatttcc accacataat atttattcaa
    18841 gagtcttatc tgcaaaatca aaggtagaag aattagataa agattttgaa ggaactatat
    18901 tagactatag tgaggttgca ggcataggaa tgaataaaaa agctcagcca tatacatttt
    18961 ttagaggtgt aatgctaagt tgggataata cagcacgacg aaatacacaa tctcgtattt
    19021 tttatggctc aaatccaaat gaatataaaa aatggattgc atccgtaatt gacttttcaa
    19081 taaattttta tgaagaaaaa gaacgtatga tttttataaa tgcatggaat gagtgggctg
    19141 aaggcacaca tttagaacca gatgaaaaat atggacataa atatttagaa gtgacaaagg
    19201 aagttttagg agcacaatat tagtagatac ctttaataaa taggttttta aatcaaaata
    19261 tatagtttta ctataattag aagttgtaga ttataatctc ttaatacaat ttgtatagaa
    19321 attgtgttaa gaggttattt acatgctaat cacctattaa tgttttctga gttaattaaa
    19381 attttcaagg aggtatttta ttgaataaac taaatagttt aattaaaaga ttatatcaca
    19441 tgttaccatt gagttatgaa aaaaaaattc tgttaaaaaa taatgtgtat aaactattat
    19501 ctccatttat aaaaaataca aatatgtata gcatttggat taatagttta gaaaatccag
    19561 aaaaatatat aaaagataat aacccaaaga agggtgtaga taatgaaaaa gatatttata
    19621 tagataattt atttaaacga gcttcagaga aatctagtaa ctatattgaa ataagtgata
    19681 caaggtttca attagaaaaa aatgatataa agctgatatc tttttattta ccacagtttc
    19741 atccaattgt agaaaatgat gaatggtggg gaagaggatt tacagaatgg acaaatgtga
    19801 gtaaagcagt tcctcaattt ttaaatcatt atcaaccaca tctaccagca gaattgggtt
    19861 tttatgacct gagattaaaa gaagttatga agagacaaat agagttagca aaaatgtatg
    19921 ggatatatgg tttttgtttt catcattatt ggtttgcagg taaaagactt ttagagagac
    19981 cagtaaatat gttattaaaa gataaagaat tagatattcc cttttgtcta tgttgggcaa
    20041 atgaaaattg gacaaggcgt tgggatggtt tggaaaatga agttttaata gaacaaaagc
    20101 actcaaaaga agacgattta gaatttatta aagatataag taattatttt aaagatgaga
    20161 gatatataaa aattgatgga aagccagttt tgattattta tagaatagaa ttattaccaa
    20221 atccaataga aacaataaaa ttgtggagaa aatattgcct agaaattggc tggaatgata
    20281 tattcattgt gggagcacaa acatttggat ttgaagaaca cattaaatat ggaatggatg
    20341 caggtgttga atttcctcct cataatataa ataattgtcc tatattaaat aaggaaataa
    20401 attttacaaa taaaaaattt actggtttag tttacgatta taaaaaatta gttgatgata
    20461 aattatatat aaaaaaatca catggaaagg tatttaaaac tgtgatgcct tcttgggata
    20521 atacagctag aaagccaaat aatgcaagta tatttaaaaa ttctacacca gagttatata
    20581 aaaaatggtt aaaagatatt atatattcta caaaagaaaa tgttttagat agtgataact
    20641 ttatatttat aaatgcttgg aatgaatggg gagaaggtgc acatcttgaa ccagatagaa
    20701 aatatggcta ctcatactta aatgcaacaa gagaagctat aatagagact aggagtgaga
    20761 aaaataatta aaaattagtt atttaaatag atgtttaagg agagttaaaa tatggataag
    20821 ttaaaaaaaa ataataacct aaaaattaaa ataatatttt ttatattatt acaaatattt
    20881 tttatatttt ttttagttgt aaatttcaaa actcctcttt tgggagaaga ttatgcttta
    20941 ctatataatg gtccatttaa agatataggt gactattttt cgacaattat ggcagctctt
    21001 gaaaagcaga tgtcaacttg gatggttaga atgggaaatc cattaacaat aatatggcta
    21061 agctttgata aaagcatata taacatagca gtttcatttg tatcaatgat tttctttttt
    21121 cttatatttt tttatggaaa tgcaagaaaa ccaaatgtga ataatctggg tgatttaata
    21181 ggtttttttg ttccagcatc acttatgatg tttttgagtc cttctatagg ggatttgttt
    21241 ttttggtgca atggcagtct taattattta tggcctcttt gtatattact tttgtgtgca
    21301 attccttata gactttattt atcagagaag aacataaata ttaagaatcc tattttgatt
    21361 attttatatt gtatagcttg ctttatatct ggttttacaa atgaaaatac tccaccagtt
    21421 attataacat tagtagctgc attgataata agagatttgt atttaaaaaa gaaaccacct
    21481 aaatggatag ttttttcctt tatatctatt tgtgcagggt ggataggatt agtagtgaat
    21541 tcaaccacca gatttagaag tatgtattat aggaatatgt ttggcttacc agaacatgct
    21601 actataaatg aacttataaa taatgtttta agagtaggat atttattctt gaaaagtgga
    21661 tttgcatttg taattatatt tttaatttta agtttgttat ttatatttat attaaagtct
    21721 caaaaaactg aaaatgagtt taaatataag catttgtttg cagaaaatat gttttttatt
    21781 ttattaactt ttatctctgc agcagcatta gtttttgcac catatacaga acttagatca
    21841 tttacactta ttcatacaat catattagca acaataagta atttattagt aaatttatat
    21901 gaaatatcta gtcataaaca aagacttgga ctgagtagta ttagtataat tactttactt
    21961 tctacttttt tactttttat taacatatac aatgaatata atgactatta taaatttgat
    22021 actcaacgta cttgtgatat aaagtcacaa gtagcagctg ggaaaactac agtatatgct
    22081 tcaaaaaatt ttattgaaaa tcgtactatg aataatagag aattttatat ctacggaaat
    22141 gatttaggac aaggtggaaa tttttactca gattactttg gagctaaaaa aataatctgg
    22201 tatgataaaa ttgatagtaa tatagatcca gctactatta atttatcaag tgaatctaca
    22261 gatgtgaatg tagaacttat aaatggaaac tctgaacaag acaatagttc tctattatct
    22321 gatgggattt ttaatgtttc tggttgggca atagataagg tggctggaaa ctctccaagt
    22381 aaagtattta taaaaataga cgataagtat tatcaagcta gaacagtaat acgtgaagat
    22441 gttgctaaat attttgacaa taaaaagtat aaaaaagcag ggtttgagtg ttctatacca
    22501 actaaagata taaaaaaagg gaagcataaa gtgacacttt gtgtcataag taatgatgga
    22561 gaaaaaagat atgaagtaga ttcaggaaaa tctatagaat ttaaataaaa gctataaaat
    22621 ggaaaggagg acatgtaaat ggcaagttta attaaacaaa gaaaatataa tagaaatata
    22681 ttattagaat atattaaatt aatgagacca aaacaatata tcaaaaacgg atttgtttta
    22741 gctgcattaa tattttcaaa caatatactc aatccatcat tagcgttaaa atctatatta
    22801 tcttttatag cattttgtat gatatcatca tcagcctata tactaaatga tattttagat
    22861 atagaaaaag ataagatgca tcctaaaaag tgcaagagac ctctagctag tggatcaatt
    22921 ggaaagaagg gggcaatttc attaggtatt gtattagttt tatcttcaat tttattatcc
    22981 ttattaatac ataaaaatct atgtgtaata atattattgt acttatttaa caatattatg
    23041 tattcactca aattaaaaaa tataatatta atagacgtat tttctatagc cataggattc
    23101 atactgagag tttgtgctgg gtctatagca ataaatgtta gtttatcaag ttggataatt
    23161 ttatgcacat tctttttatc tttatattta ggttttggga agcgaaaaaa agaaataata
    23221 ttacttaaag aagatgcaag taatcataga aaaatattaa aagaatatga tgaagaaaac
    23281 ctaaatcaaa tgatgtcaat atctttaagt gcgactatag tgtgctactc attatactca
    23341 gcaaataaca tttataattc aaatatgata tttactactg tgtttgttgt atatggtgtt
    23401 ttaagatata attatatagt taatataggg gatgaaggta atcctactga tgttgtatta
    23461 aatgataaaa gtttaaagtt ttgtgtttta ttttgggtaa ttgcgtgtat tggaatttta
    23521 agtacttaga gttaattaca tattaaaata aaatttaaag ttgaaaagat ggagaatttt
    23581 catgataaat atagtaatac caaattataa tggatataaa cttttaaaaa attgtctaga
    23641 ttcattaaaa aatcaaacat attctaattt tagtataata gtagtagata atgcatcaga
    23701 tgatagttca tataattggc taaacaacta tgaaaatgtt acactgaaaa aattagataa
    23761 gaactatggt tttagtaagg cagtaaatga aggtataaaa ttatctaatt cgaaatatgt
    23821 tgttttgtta aacaatgata cagtagtacc tgaaaattgg ttagagacac tattaggaga
    23881 gattgagaaa gacgagagta ttttttccgt atgtagcaaa atgattagat ataatgaaaa
    23941 ggacaaaata gatgatgctg gagatgaata ttctatacta ggctgggctt acaaaattgg
    24001 agatggtaag aacatatcaa gttttagaaa aagtaaatat gtatttagtt cttgtgcagg
    24061 tgctgctata tatagaaatt caatttttga tgagataggt tattttgatg aagatttttt
    24121 tgcatatatg gaagacattg atatatctta tagagcaaaa atttatggat ataaaaatag
    24181 atattgctca gaagcaaaaa tatttcatat agggagtgct acatctggaa gtaaatataa
    24241 ttcatttaaa gtaagattgg ctgctagaaa caatgtatat gtaccatata aaaacatgcc
    24301 ttttttacag cttataataa acatgccttt tttaattatt ggttttttag tgaagtactt
    24361 gtttttcttg aaaaaaggat ttggaaacga ttataaagag ggtttttttg aaggtatcaa
    24421 gaatttaaag aatctaaata agacagaatt tagaataaaa aatttattta attatttaat
    24481 tatagaagct ttattgatat tgaatatgtg taaatatata ttttca
//
LOCUS       DAMJYC010000054        24420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJYC010000054 DAMJYC010000000
VERSION     DAMJYC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.055"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            138..638
                     /locus_tag="PC472_002385"
     CDS             138..638
                     /locus_tag="PC472_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2727704.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(888..1667)
                     /locus_tag="PC472_002386"
     CDS             complement(888..1667)
                     /locus_tag="PC472_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727705.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1664..2143)
                     /locus_tag="PC472_002387"
     CDS             complement(1664..2143)
                     /locus_tag="PC472_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2727706.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAINNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2952..3653
                     /locus_tag="PC472_002388"
     CDS             2952..3653
                     /locus_tag="PC472_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2727707.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            3679..4335
                     /locus_tag="PC472_002389"
     CDS             3679..4335
                     /locus_tag="PC472_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727708.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            4440..6080
                     /locus_tag="PC472_002390"
     CDS             4440..6080
                     /locus_tag="PC472_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2727709.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6438..7760
                     /locus_tag="PC472_002391"
     CDS             6438..7760
                     /locus_tag="PC472_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727710.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIGTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7957..8964
                     /locus_tag="PC472_002392"
     CDS             7957..8964
                     /locus_tag="PC472_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2727711.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            9002..10429
                     /locus_tag="PC472_002393"
     CDS             9002..10429
                     /locus_tag="PC472_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2727712.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            10571..11836
                     /locus_tag="PC472_002394"
     CDS             10571..11836
                     /locus_tag="PC472_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727713.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11878..13476
                     /locus_tag="PC472_002395"
     CDS             11878..13476
                     /locus_tag="PC472_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2727714.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            13511..14533
                     /locus_tag="PC472_002396"
     CDS             13511..14533
                     /locus_tag="PC472_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2727715.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(14991..16394)
                     /locus_tag="PC472_002397"
     CDS             complement(14991..16394)
                     /locus_tag="PC472_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2727716.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            17567..18076
                     /locus_tag="PC472_002398"
     CDS             17567..18076
                     /locus_tag="PC472_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2727717.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            18104..18298
                     /gene="rpmI"
                     /locus_tag="PC472_002399"
     CDS             18104..18298
                     /gene="rpmI"
                     /locus_tag="PC472_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2727718.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            18354..18710
                     /gene="rplT"
                     /locus_tag="PC472_002400"
     CDS             18354..18710
                     /gene="rplT"
                     /locus_tag="PC472_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2727719.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            19020..19565
                     /locus_tag="PC472_002401"
     CDS             19020..19565
                     /locus_tag="PC472_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727720.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            19552..19725
                     /locus_tag="PC472_002402"
     CDS             19552..19725
                     /locus_tag="PC472_002402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727721.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            20000..21925
                     /locus_tag="PC472_002403"
     CDS             20000..21925
                     /locus_tag="PC472_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2727722.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            22326..24230
                     /locus_tag="PC472_002404"
     CDS             22326..24230
                     /locus_tag="PC472_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2727723.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         9144 a         2739 c         4433 g         8104 t
ORIGIN      
        1 agatatattg gttttaattt tatagaaata tacaaagata ttttaagcta atatatagta
       61 atttatatag cattataaaa tagaatcata taaaataagt taactacaat caaaaattaa
      121 aaaggggaaa tgttaagttg atagaaggca taaaatttat aaaggcagaa gaaaagtata
      181 tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat ctagctatgg
      241 atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatattata aataaaaact
      301 taccacaact gtttataatt gatttagaat cagataactg tattggttgg tgtgacgttt
      361 tacctaaaac tgaaaaagtt ggttatctag gcatgggtat tcttaaagag tatagggaaa
      421 aaggtatcgg aagtagtctt ttaaaacaaa ttattgattt atcaaaagaa tatgggtatg
      481 aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt tataaatctt
      541 tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat agacctgtaa
      601 aagaagaagt aattcaaatg gagcttacac ttatataaaa taaagttaaa gtctttaaaa
      661 ttccatatac acttgtataa aataaagtta aagcccttaa aattccatat aaaagaaata
      721 tttttaaggg ctttttaata atggttcttt tgaaggtagc taaatatcta aaattttata
      781 tattaaattt aaagaaaata taaagatgga agatacaacc atgtttacta ataaaaaaat
      841 taaagtcata aataataaac tcataaacaa cttattatta cttatgttta ttttgcatct
      901 attacttcat gagccatttt aactaagtca ttcatagata aatttgagtc ttgaactgta
      961 aaagaatagt aaactccatc ttgtttccat gtcaaaaact taaatttatt aacttcaact
     1021 ttgtctgatt cattatttgg ccccacacta aatgtgtatt tgcctgattt ttcatcaatt
     1081 ttatcctgtt ctgtcatttt ataaccttct ggtacaaact tttgtgcgaa tgcttcataa
     1141 gaaatattaa tatctttgta agtatctaca acttttagat tgtcagaact cttcccaagt
     1201 acatcatttg ctgtgtcaag agatatttca tcattgccat ttttatattc aaaactaagt
     1261 gcttttgatt ttcctaaaac attattttct ttgtcaaatg cttttttatt tacagtatat
     1321 gctcctttaa atttatatcc attactaaat tcagaaacta tattaggggt aaagttaaag
     1381 tcttttttta cttgttctgt agttgggata gatgtatagg tagccttagt acttgatttt
     1441 ccaactaaac tatatatatg tcctactgca agggctgttg tgctcaatat caaagtaatg
     1501 gctaaagttg caactaagcc ttttctaaat ggtaatttgt gtgttttatt attaatcatt
     1561 ttataatttt cctctctttc tggtaaacta tttagtgttt tgcttaaact attatgaaaa
     1621 ctttctggaa cttctggaaa cgaattattt aatatatttt tattcatatg attacctccg
     1681 tgttttctaa tttagttttt aataatcttc ttcctcttga taatctactt tttacagttc
     1741 cttgaggtat atctaatatt tccttaactt catctactga ataaccttca atatagtaaa
     1801 ggactattgg aattttaatt tttttaggta gactttgaat agcagtatac aaatcgctat
     1861 agtcatcttt ttcatcaatt tttttgtttt tgaagacatc ttcaaatgat agagatttta
     1921 atcgtttgcg tcttaatgaa ttacattcat ttatgactat tctaatcagc catgttttaa
     1981 agtattgttc ttccttaaga gaatccaact tttcatatgc ttttaaaata gcattgttga
     2041 tggcatcttc acagtcttgg tcgtttccta gaatagactt agatactcga tacaaagtct
     2101 gttctgattc aagtatattg tttgtaaatg tagttttatc caactaagtt ccttcccttc
     2161 tatgcaaatc gtagcgctac ttttttgcct ttcacttatt agataaaatt ttactataga
     2221 tggttcactt tttttatgat tatttgataa atttaagttt gaaagttttg cctattctat
     2281 acaaatattc tataatatat ttttttctag tattttcccc tcatatataa agccatgtag
     2341 atatattaaa ctttagaagc catttatata tttagtttta tagtaagtgt ttatattgag
     2401 taaattagaa aatagtaagt atttaatgtt gagtagttag aataacaatg cttttggatt
     2461 atttacttca tgtacagatt taaaataagt tttttaaata atgagtaaag ataacagtat
     2521 cttgactaaa agtaggcttt taagaaaaaa caatgaaact taaatgagaa ttaagagtta
     2581 gaaatgtcat tattttagat agagatgaac ataaataaac ttaaaaaata taaattataa
     2641 aaataataat tttttttaaa ataataaagt tttttgatta aaagttttat attgaaatgt
     2701 caaaataata aaagacctta taagttaatt acaggttaaa aaaataatat gtgaattttc
     2761 tgatatttac taattatgaa taaaatattt aagtattaat gtttttagtg agactttaag
     2821 acagataatc tcctattaaa gatactatat cttagataaa ataaattaaa acaatataaa
     2881 aacataacaa gattacagaa agaaagctca caataaaatg ctgaaaaatt tattttaagg
     2941 aggaataaag tatgaagttt caagaaatga ttaatagccc aggtctttgg attgtttcta
     3001 gttttttagt aattatttca gttgttcaag caatcgtatt tatgaaagag gcactaaaag
     3061 aggcttcaaa tcttggtatt gaaaggaaag gaattaaagc agcaattcgc tcagctagtg
     3121 ttacagccat tggaccttct ctatctcctg ttattacatt attatcactg gtagctgtga
     3181 ttggcgctcc tacgacttgg atgaggctct gtgatgtcgg agcagctaga acagagcttg
     3241 gagtaatatc tcttacatcc aatttatctg gggtagaagt aggtagtgct gcatttggag
     3301 cagaagcttt ttcgtatgca ctatggggga tggctttaaa taacttaggt tggctatttg
     3361 ttgtatttat tttgggtcat agaatgcgtg gaattgttga aaagatgaac atgaagtata
     3421 atcctacttg ggttaaaaaa ctacttgcag gagctacagt tggattattt gcttaccttc
     3481 tttcaaatca aataaaaaca tttgaaatac caaaacttac accagcaatt atttcagcta
     3541 tagttatgct agtattgaca actttattta aaaagaatca acgattacaa gaattatcct
     3601 taggtattgc catgttaatt ggtatgtttg gtacacagat tatattatcg tgattaaaac
     3661 ggataagagg tgataattat gtatgataaa aagatgataa aaatagggag aatttctata
     3721 ggtcttgcaa ttattgcaaa ctttattccc gctatctatg taggtattcg ctttggtcag
     3781 atgccaatgc tttctgttat atttcaaatt tgggggttag tcgctgcgac ttatggggtt
     3841 agttggattg ttcaacctat agcttattat cctacactag gagctccagg ttcttatata
     3901 ggttggcttg caggttctgt tggagatata cgtatgcctg cagcatcaat ggcacagaaa
     3961 attgcaggtg tagaagcagg aactcatgag ggtgaagttg ttggaacaat tggtacatgt
     4021 tgttcaatct ttgtatcagc ttctatgatt accattttta ccattgtagg ctataaagta
     4081 attcctttgt tacctgaatt tgttactaat tcatttaatt atatacttcc agcattattt
     4141 ggagcaattt atgttgatat tgcacgtaag gatattcgag ctggtggatg tacgattgct
     4201 gtagcgttag caattatgta ctttggtgaa aaaattggta taccaggtgg attattaaca
     4261 ttgtttattg tgttaggtgg gatacttatc acaagggtct tctttgtaat tgataacaat
     4321 aaaacaaaag aataggattg cttattaagg agaaagacta gaattatttg ttgagaaaaa
     4381 agaataggat tgtttattaa gaaatatgaa tataaataag tcttgaaagg agaaaaagca
     4441 tgtttgatgt taaaattgtc aatggaataa tcgttgatgg tacaggaaat tcaagatata
     4501 aaagtgatgt agggattgta ggagacaaaa tcatggctat tggagattta agtcaaaaag
     4561 aggctaaaga gactatagac gcaactggaa aatttgtatc acctggtttt atagatttcc
     4621 atactcattc tgatttatct cttgtgtatg ataaatacac aagaagccgt atccatacag
     4681 gtgtaacaac agatgttatt gcaaactgtg gtataggagt tgctccgata agagaagaga
     4741 agaagcaaga gttaattgat tatcttggaa ctaggataat tggtacaatt ccaacaaaat
     4801 tagagcttca ttggaataca atgcaagaat actttgatta cttaactgaa aattctccag
     4861 cagtcaatgt tgtagcttat gtagcacaag gacctgtacg tatcaatgaa atgggatttt
     4921 caaaggaacc agctacttta gagcaactta aaaatatgaa gttggaagtt agaaaagcta
     4981 tggaagctgg atgtgtagct ctttcatcag gattagttta tttaccagga gcatacacaa
     5041 agaaagaaga aatggctgaa ttatgtaagg aattgatacc atacaatgga tactatatat
     5101 ctcatattag agatgaaggt gatgaagaaa tggaagctct tgatgaagca atttatatag
     5161 ctaagactgc aggagttcct ctacatgttt ctcatttaaa agttatgggt cataagaatt
     5221 ttggaactat tgatgaggta tttaaaaagc tagatgaagc tgaggcagat ggtttggaag
     5281 taacttttga ttgttaccca tatacagctg gaatgacatc tttgggtgca cttttacctc
     5341 cttgggcttt tgaaggcggt gtagaaaata tggtaaaacg attagaagta caagaaaata
     5401 gagacagaat tataaaagag ttggaggaag ggattcctgg atggcaatgt ttctaccaat
     5461 tagcaggtgg gtggaacggt gttgtactag cctctgtaat gactgaagca aataaatatg
     5521 tagagggtaa aactttaatg gaggtagcca aaatcaatgg tgaaaatcca tttgatacgt
     5581 tctttaggct tttgattgaa gaaaaaagta aaattcaagt agttgttcat acacaaggtc
     5641 aagaggatac agataaggtt gtttgccatc caaagtcatg tataggttct gatagtatgg
     5701 acctttctac agaaggttta ctttctcttg gaaagcctca tcctcgtgca tttggtactt
     5761 ttggacgaat tttctcttac tatgttagag aaaaaggtat gttgactttt gaggaagcag
     5821 ttaaaaagat aacttatctt tcagcaaaac gcttaggcat ttataaagaa aggggtctac
     5881 ttaaagaaaa ttattttgct gacattgtgg tatttgaccc agatacaata gaagataaag
     5941 cgacttatag taatcctaaa cagtatacag tgggagtgga atatgtgttg gtgaatggta
     6001 aaattgcatt ggcaggagga aagcaaactg atgtatgtgc tggacgtgta attaaaaatc
     6061 ctcttagcat agcaaaatag aatacattaa gaacttttat aaaaatagtc tttctttgaa
     6121 tattaaggaa ggctattttt gttaaattaa taaagattac tctacttgtg ttttctaaag
     6181 aatttaactt taagtagaaa aaacaatata ttaattgtaa aatgtaatta atattaatga
     6241 ataagattta aatacaaaaa tttaaataca aaaatgtttc aacagatagt cgtaattaaa
     6301 aaacttaaag taacttttta tattaggtat aattgattat tcaaaattct atgaatcagg
     6361 agtcctgtgc ctttagtcat ggtaggttta gaaaatgttt taagagataa tagtatcatt
     6421 aggaaaggag agacgaaatg aaaatacttt atgtaggacc aggtatggta atcgataaaa
     6481 tactagcagt aagtaaaaag aattttccta atattgaagc agattttctt agatatgata
     6541 gatatattga agtacctgat ttattggaaa aatatagagg tagagtggat gcagttcttt
     6601 ttacaggtaa gtcgccattt aaattttttg aaaaaagaca aaaagatttt gtattgtctg
     6661 attatattcc tagacatgaa acaacattgt acagagtgtt gcttgaaatt acatatttgc
     6721 ttaaattcga tattagtaga cttagtatag gtacttatga taaggctatg atgaagagat
     6781 tgtataaaga aattggagtt gaatttaagg atgaacatat gttttttgca gagcaaaagt
     6841 atttagaaga ggactatata aagtttgtgc ttgaatttca taaatataat tatcaaaata
     6901 atgatgtatg ttgttgtatt acaggtcttg aagaggccta tcatataatg aaaaaagata
     6961 atattccagt agttttagcc ttgccttctg aggacgtaat ggtgcaaagt ataaaaaaca
     7021 ttcagatgcg ttatattgca aagaaaaaca atgagaatca gattattgtt cttgctatta
     7081 aactgaacat gccttcagaa tattcactaa tgaaggaaga tgagtacgct tatctttcac
     7141 aaaggattaa gattcttgat aaactatacc atttcaatag ccgtatagat ggtgttttgg
     7201 tagagcagag tagaagtgaa tttatgattt ttacaactaa aaaaatactt gagttagaaa
     7261 caaagaaata cagagatatt tatttgttgg atatgattcg tgaagtttca gttataaaca
     7321 cttatatagg aattggttat ggaaaaacag caaatgaagc taaatttaac gcttatgaga
     7381 gtattaagat ggctcaaagt caaaaaaatg atgcagcata tgtagttttt gaaaatggag
     7441 aaattatggg accactaaag ccaaatccta ctgtcaaaga gaaaaatagt tttgatgaac
     7501 gtttttatca aattgctaca gagacagggc taagtgctaa tactgtatat aaagtattca
     7561 ataaaataat aaatagtgga aaaactgaat ttacatcaaa ggaattagct actatatgtg
     7621 gtgtaagtat tagaactatg gacaggatta tacttagatt gtgtgattct ggttattgtg
     7681 aggtagtgag tgaacagctt atgagcaagt atggtagacc cagtagaata ttacgtttta
     7741 aaccttttct gttgttttaa atagatataa gtcaaaaatt aaatttttat gataacattt
     7801 tctttacaac tttttagata tatgttaata tttagaaaaa tgcatatgta ttcataaaat
     7861 aaactcaaaa cagctaaaaa atcattaaaa aggaaatcgt atgcataaaa tgagaaagtt
     7921 aatttataac ttaaataaat ctgggaggag ataattatgg gaaaatatca ggattcaaaa
     7981 aaagttgtaa gaaattattt tgaagcatta gaaaatgcta ctggggatga ggttgaaaat
     8041 gtcttaaagc aacatatgaa atcagattat aactggaaag gtgtgtatcc atttcgtgaa
     8101 caagaaggca ctgaaaatgt agcagatgta ttttggaagc ctttaaaaaa atcattgagt
     8161 aatatgcaaa gacgacaaga tattttcatt gcaggaacaa atgagattga caatagtgaa
     8221 tgggtaatga gtatggggca ttttatggga ctttttgata aggattggct tggcattaaa
     8281 catactcgca aaatgattag tttaagatat gcagaattta actgtgttca agatggtaaa
     8341 attgctaaaa ctggattatt tgtagatata attgggttta tgattcaagc aggagttaat
     8401 ccattgccac cacaaacagg ttcatatttt gtatatccag ggccaattga ccataatgga
     8461 ttactttttg aagatgcaga tgaagttgaa ggagtaaaaa ctttagcact agtaaataaa
     8521 atggtagatg atttatctga attaaatgaa agtggtgcta tgggatgccc accagaaata
     8581 cttgaaaaat cttgggctaa agatatgatt tggtatggtc caggtggaat tggtgcttct
     8641 tatactatac ctagatatca agagcaacac cagctaccat ttagaaataa tttaaaagga
     8701 aagactttta atgggcatgt atgtagattt gcagaaggta attttgcttg tttctttgga
     8761 tggcctaatc tgactaatgt accatgtgga ggttttttag gattaccagg tggagaggtt
     8821 aaagcagata tgcaagttgt tgatgtttat tatcgtaagg gcgataaact tcaagaaaac
     8881 tgggtgttaa ttgatattcc ttattggttg aaacaacaag ggcttgatat tcttgagaga
     8941 actaaaagta tttttaatgc ttagttatgc atacgtattc taaaacaaag ggggatataa
     9001 agtggcatct accaacaaaa ataatgcaac aagtaaagag gaagaaaaag aagtttctac
     9061 aagtgggctt gaatcatgta cggttaaaga aaacaaagct aaaatgaatc aaactaattt
     9121 tagtaatata aatgaaaatc aaacaaatga gaataaaacg aacacatata aaagaaatat
     9181 agataaagga aatatagata aagggaatat agataaaaca aacacaaata aatccgacat
     9241 aaataaagtt aacatagata cagtggatac agataaatta aatacaaata ctcttactga
     9301 aaaaaataaa aaagttatat ttaaggggga agataatatg tcatctacaa aaatttacta
     9361 tggagattca ttagaagtaa cgcctgttgg agtaatggat tacaatgatt tttcaaaaca
     9421 gacaaaaagg gaacaggaaa tacctggatt tgattctaag tatagagatt ttgtagatta
     9481 tattatgaag attacacata atatctggga agaaaaaggt ataggtgtta tttatgatac
     9541 atatcacaat aatgttacaa tgcattgtgg ctcttcaaat ttagttggta ttaaagatgt
     9601 tatttccaat actcttcaaa cattacatgc gtttcctgat agaagattaa ttggtcaaaa
     9661 tgtaatatgg tctaactttg gtgctgatgg ttttttatct tctcaccgtg ttttgtctac
     9721 agcaactaac ttaggtgata gtaattttgg accagcaaca ggtagaaaaa taaactttag
     9781 aactgtaatt gattgtgcca caactaacaa tagaatccat gaagaatggt tggttagaga
     9841 taatttatgg attgttactc aattaggatt aaaccctcag gaagtagcaa agagtatggc
     9901 aaaggcaagt gaatctaaag tgcttagttt acaatcaact tatggtatct gtgaatctat
     9961 ggatggtcaa tttatgccaa ctaagtatca agcaaaagat gattctgttg gcgaaatgat
    10021 gcttgaaatg acaagccgta tttataatta taaatatata aatgaagtta aaaaatacta
    10081 tcatgataat gcagtagtac attttatatg tgataaagat ttaaatggct atgatgagat
    10141 tcaaggaatg attgtaagtt tatttgcatc aattccaaat ggaagttatg aagtggaaag
    10201 agtaacttgt aatcaaagag aaaaaaatga aggttatgat gtatctgtaa gatggagatt
    10261 acgtggaata aatgaaggta taggattttt tggacagcct tctggaaagc atatagaagt
    10321 aatgggaatc aatcattatc atatactaca aggtaaagta aaggaggagt ggataacttt
    10381 tgatggtatg gatgtattaa aacaaattta tatgggagta gaagaataaa gctgtttaga
    10441 gtataaatag ttttgaaata tatagggaag tattgcttta taaaatttaa gtggggaatg
    10501 tatatgaaaa atagatgttt aaacttttag caaaattatg actaaattta aaagggggaa
    10561 ttataaaaat atgaaaacaa cggttgaaat agaaaatgat atcatattag gaaaagacta
    10621 ttcaaaccta aaaagaggaa ttgcattttt ttcaattgct ttaatatact ttttttattg
    10681 ctacaatttt atggtgggta cttttattaa accaacaatg atttatgctt tagcagatgg
    10741 gggatttggt ttttcattaa aacaaacaga agaaatattt gcagttatgt cttttggtac
    10801 aattccaggt acatttattt ttggggtgat ttctacaaaa attgggaaga agaggacttt
    10861 aattagcgtt gctttactga ttggattgac aacatttata ccaatgttat ctcctacaaa
    10921 tattatgtta tggaaaatag cacgtttgtg tacaggagta gtgttgggag gcgtatttgg
    10981 aacagctatg cctttagttg cagatatgtt tccaagtaaa tatcgtggta agttagctgc
    11041 tatattaact gctttatttt cattagcaat gatttttggt gggaaagtat atggcgtttt
    11101 aggtgatgca aattggcaaa tattgatgta tactgcgatt ataccaccta tagttggagc
    11161 tatcttagca atcttctttg tgccagatga tttggagtat acaaaggaat tagttaaaaa
    11221 gggaaaagaa actggtgaaa agattagcta tataagtatg tataagggaa aatacttatg
    11281 gattggattg ggaacgattc ttttatcagg tgcaaatttt acagcatatt cagcattttc
    11341 aaataatgca actacatacc tagtgacagg aataggaatg tcagcagcag tggctgagtc
    11401 tatatatagt ttacaaggaa ttggtcaatt agtaggatat ttattctggg gttcaattgc
    11461 agataaattt ggaagaaaaa taccttttat aggtatggca ttagcagcag tatttgtttt
    11521 tatatataca aaattaggtg gagataatac aacaacattc tatatgatat cagtattact
    11581 aggtgttgct gttggatatt ctggagcatg gggagcatac tacacagagt tgttcccaag
    11641 taaatatcgt tcgttgtcat ctggtatgtc atttaatgga ggaaggatta tatctacgtt
    11701 tgcaatacct gctatagctg gtacagcttc tggtacttta ggtatgatga ctatctttta
    11761 catatcaatc gcagtctttg tagcaggtgc tattgtatgg ttattcttac cggagacgct
    11821 aaataataaa aaataaaata ctaggaattt attttgttac ttagaaaggg gtggcttatg
    11881 atttcatcaa ttgatgtatg tattataata ggatatttgc tattaatgtt agttattggt
    11941 tattattcag gaaaagataa taaaaatcaa gaagattatt tcttggcagg taggtcaatg
    12001 ccatggattc caattgcttt atcagttgca gctactatga ttagtgcaaa tggctttata
    12061 ggtggtcctg gatgggcata tgtagatgga atgtatccag taatggttaa tattacagta
    12121 cctttagcaa tattttttgc actgagcatt accaccccag ttatctataa attaaaaata
    12181 acctctatat atgaatatat gggacttcgt ttaggaaatt attcaagagg attaactatt
    12241 attcaatttt ttattaattc attgattcaa gctagttcta tggtatatat tccagtgctt
    12301 attattcaaa tgattactgg ctggtcactt tatgctttag tcccaatagt tgtacttgta
    12361 tcaattatat atactctatt aggtgggatt aaggcagtta tatggactga tagtattcaa
    12421 atgatagtag taataagtgc tgtatttata gtaattattg tagccttaaa aggaatgaat
    12481 ctaagtttct ttgatacatt acaaattgca caacagagtg gaaaattaaa tacatttgat
    12541 ttcagtaggg atatttcagt gacaaatact ttttgggcta ctttaattgg tggaactgta
    12601 atgtggataa gatacttttg ttttgaccaa tctcaagtac aaagagtttt aacatctaaa
    12661 tctttaaaat gtgcaaaaaa ttcttttgta gttagtgcat ttgttatgaa tttagtttac
    12721 tactttatgc tattggtagg agtaatttta tttgtttttt atgggggaag gacatttgaa
    12781 acttcaaatg aaataatgat tacttttatt ttaaatgaac ttccagtagg agccattgga
    12841 attattattg caggtgtgtt tgcagcagca atgtctagta tcgactctat attaaacagt
    12901 atgacaactg tttttacgaa ggatatttat gagttttatt tcaaaaaaga caacaaagaa
    12961 tcatcattaa aagttacaat gattataact gtagttatag gagttgttat gacaggtttt
    13021 attattatgg gatttggagg aagcatcaaa tcaattcttg accttgttgg taactatatc
    13081 tcatactttg ctggaccagc tacaggtgca tttgtactag caatgtttac atataaagct
    13141 aatgataaag gagtatctat tggttttatt gtagggttag ttttaggatt ttttatttct
    13201 aataaatata atgtgagttg gttgtggaat ccagcaatag gtgctacaat aacattgata
    13261 tttggatata tttttagtgt cattttaaag aaaaatcata atgcagatga tattaaaaaa
    13321 tacactgcat ttggtatgag aaatcacctt atttctcaag gaatggataa agtagatgga
    13381 gtatctatag tgccattttc tttaggtaaa caagaactaa ttgtattagc ttttttcatt
    13441 atccaatata ttatattatt tttgatacaa ttttaagaag aaatactaga tatttaagga
    13501 gaaatactaa atgaatcaaa agaaagttca ttataataaa tcggctacct caaaagatgt
    13561 tgcaagattg gcaggagtat ctcaatcatc tgtttcaaga gcatttggta gtgcaaatgg
    13621 aaagggcgta aaacctgaag taagagaaag aatttttcgt gtagcaaacg aaataggata
    13681 tgttcctaat ttggtagcac ggggaatgat tagtggaaaa actaatgtta taggattgat
    13741 agttggtgat agtctaggtc cattttacaa tagaattatc aatttatttg tagaaaaaat
    13801 tcaagagata ggtaaacagt gtctcgtttt taaagttcct agacaacaaa gtatagatag
    13861 tataatttca aaagtaattc agtttcaagt agaagctgtg attattacag cttcggccat
    13921 gaataaggtg atggctgaga cttgtgaaga aaacaatatt cctgtgattc tttttaatcg
    13981 ttttatacct ggtataaaaa taagtacagt ctatgtagac ccaattgaag gtgggggaat
    14041 ggctgctgaa tatctgctta agaaaggtca taagaatata ggatatattc aatttacgag
    14101 agaaacaagt gaagaaatgg aaaagaaaat tggattttat tctaagttaa gacaaagtgg
    14161 gattcataac cttaaagaag aatctgcaaa ctatgattat gattctggat atgaagcagg
    14221 taagagaatg ttagcattat caaatcctcc aacagcaatt ttttgcacaa gtgacttgat
    14281 agctattggt gtaatggatg ctgcaaggtt tgagtacaag ttgagggtgc cagaggattt
    14341 atcagtgata ggatatgatg atattcaaat ggcttcatgg aagagttata atttaacgac
    14401 gattagacag ccactagatt tgttgattga aaaaacaatt aacatactta agtccttatt
    14461 aaatgaagaa aatccagagt cagttgttga aatgttaaaa cctgaattga ttgaaagagg
    14521 ttctacactt taagtggtag atatttaatt aagtgataga tatttaatta agtggtagat
    14581 atttaattaa gtggtagata tttaatcaag tgatagatac ctaattaagt tgtagatatc
    14641 tgataagaaa tttttaagtt ttcaatcgtt ctaaattgtg agtataaaaa tctactaaat
    14701 ttagttatgt aaaatataaa ttataagtgt agaaactagg ctactagctt ttataccact
    14761 tcaaacatta ctggaggtag ttaagtttac agcagtttag ttttatgtag atataatatt
    14821 ctgtaaatag acaaaattaa aatatgtaat taaaatatgt aagtgtctca aaatgaaatt
    14881 tttagtttat gaggcacttt tttgtataaa atcaaatgta tttttataac tttaacaata
    14941 aaaagaggct tatctctatt ttgagacagc ctcaaatttt taaaactata ttagtttaca
    15001 gcttcttttc ctatcaaatc ttttaactct tcatgagtca tagtttcttt ttcaaataag
    15061 aatttagaaa cagaatgtaa atcttcaaga ttatccttta acatctctaa agtctcttta
    15121 taacaaattt caactatacg atttgcttcc ttttgaacct tatcacataa gaaagtctta
    15181 tcatttccat caatagtcat aaatcctaag ttactcatac catattcaca aaccatttgg
    15241 ttagctattt cagtgacttt ctttaagtcg tcctttgcac cagtagattt atgattaaat
    15301 attaattctt ctgaagcctt tccagcaagt aatattttta tttttccaat caactcatcc
    15361 ttagtataaa tatatctatc ttcatcaggt aattgaagaa cataacctaa tgcttgtcct
    15421 ctaggaacta tagatatttt ttgtatagga caaattccta ctatatcact tacaagagca
    15481 tgtccagctt catgatatgc tactattttt ttctcttttt caaccaatgc agagttttta
    15541 gattctagcc ctgcaataac tctttcaagt gccttgtcaa aatgttctga tgtaatacac
    15601 tcactgtcat ctctaactgc aaatatagct gcttcatttg ctatatttga aagatgagcc
    15661 ccattgaaac catgagtttt tttagctaaa agttctagat ttacagattt gtctataggt
    15721 ttgtcatctg tatgtacctt tagaatttca aatctagtgt gatagtttgg tgcacctata
    15781 tgaatgtgtc tgtcaaatct tcctggtcta agtaaagctt catctaataa gtcaagtcta
    15841 ttggtagcac ctattattaa tacattggaa tctttattaa atccatccat ttcaactaaa
    15901 agttggttta gtgtttggtc tttttcgttg ttactttcaa gatgtctctt tgctcctaca
    15961 gcatctattt cgtctataaa tattatactt ggagcttctt ttctagcttt ttcaaataga
    16021 gttctaactc tttttgctcc cacacctaca tatttttcaa cgaactctga accagtaaca
    16081 ttgaaaaatg atgagtttgt ttcaccagct acagcagatg caagtagggt tttacctgtt
    16141 cctggaggac cataaaataa tactccttta ggtatttttg cacccatttt ttgatatttt
    16201 tgtggtgact tcataaaatc gactatttca aatagttctt cttttacttc gtctaatcca
    16261 gcaacgtctc taaatgtagt ctttggtttt gtagaaagtg aatcatctgt ttgattatct
    16321 ctttccattt ttgtctgtgc aaagacagga gctggattgt tgattttctt aaaaaagttg
    16381 tttagcattt tcatattcac acctcaggta attaattatt gttaatcaat tttatatttt
    16441 ccatatttac tattttttat tatttatttt tcatggaaaa aatatacatg ttatctaaat
    16501 aaaaataaat ttgtaagatg tatgaaaaaa tgaatttatg tatatagaga tttctactta
    16561 tgtattgaat aaagtattac aaattataaa ttatgtattg attatatatt gagtaaagta
    16621 ttataaagca tgtattgatt aagtatttat taagtattga ataaaatatt acaaatttag
    16681 ttgaaatgaa aatgataaaa aatgttgtac tatattttac aaatcatata aatagagaaa
    16741 ttgttgtagc acaaagtagt aggtagtata ttagaatata attataggat ttgttgtgta
    16801 gtatagtagc atattagaat ataatatagg atttgttatg tagtatagta gcatattaga
    16861 atataatata ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt
    16921 tatgtagtat agtagcatat tagaatataa tgtaggactt gttatgtagt atagtaacat
    16981 attagaatat aatgtaggat ttgttatgta gtatagtagc atattagaat ataatgtagg
    17041 acttgttatg tagtatagta acatattaga atataatgta ggatttgtta tgtagtataa
    17101 tagtatatta gagtacaata tagaatttgt tatgtagtat agtagtatat tagaatacaa
    17161 tatagaattt gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta
    17221 gaatatatag agtcatgtag aatatacata tataaaagga ttttgggtat aaatttaaaa
    17281 attaatagca aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt
    17341 cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaaa taaggtaaga
    17401 ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa
    17461 gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt
    17521 acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt
    17581 agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca
    17641 acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt
    17701 ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt
    17761 tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt
    17821 aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa
    17881 tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag
    17941 agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa
    18001 agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat
    18061 agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac
    18121 tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa
    18181 agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa
    18241 atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga
    18301 tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa
    18361 gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag
    18421 gattcagagg atctagaagc aaattatata gaccagcaaa cacattcgta atgaaagcat
    18481 taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac
    18541 aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat
    18601 taaaattatc tggtgttgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc
    18661 cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca
    18721 tgagatgtaa agtctttgag ttatataaat aattcaagga cttttttatt ttttgaaaaa
    18781 tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata
    18841 gaattttatg agatttatta taagaattgt tacaattatt tatgatagct ataaaaaatc
    18901 atagtaaaaa tgatactgat gtatgttgat atatgtataa agtttccaat caatcttgaa
    18961 ttaatattat caacatgata atattatctt attaataata taaataaggg ggagataata
    19021 tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa
    19081 ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa
    19141 aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtagttg
    19201 aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg
    19261 atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc
    19321 aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata
    19381 tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag
    19441 ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac tcgcacgaag
    19501 aaggcagaag aatgcattct ataggagtga taatcactcc acctaaagag gatgaaggga
    19561 aataaatctg atttacatga ggtagagcca atttacacag aaaatcgttt tatgatttac
    19621 atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtacc atttcatcca
    19681 atgacttgga gtggtacaat gaagtttttt aatttttcat attaatgcta aagaaatgaa
    19741 ctttagacca catatgcaat taggttgcat tatacaatta gaccacatat acaaaataaa
    19801 aatcagtatt tttatagcag ataaattgac tgtcattctt cttaaaaatc atctattagt
    19861 tattgttagc atattgaaag aaattatagt aatctattga ataaatgaat attgcctata
    19921 atcatataaa ctgctatagt ttaaaataaa gcaggacgtt aaattttata gtaatctaaa
    19981 aatttaagag ggggatttta tgaaaataaa atttgttatt atgccattgg tgatattttt
    20041 aatggtatta tttgtggggt gtcaaagtac aaaagataca gaagttaaag caaaagatac
    20101 agaaattaaa atattaggga caggtgattt acattcaatt ttgacagact ctatggtttc
    20161 gtatgtaaat gaggaaaggg aaaagaataa aaatctgctt atggtagatg cgggagattt
    20221 ctatggtaca caaagtagag aaatgtggga atggtcttct ggaaaaaagt taattaatat
    20281 taaaagaaat ggtagagcag agtatgatga cataattaag agtagtaaag atgaagtacc
    20341 tatagttaaa gatatggcaa agttgaaata tgatgctgta gttttgggag ataatgaatt
    20401 tgtttcaaat gataagcaaa gtcttgataa actggtttct gattttaaaa ataataatat
    20461 gccattggta tcagcaaata tatatgaaca atctggagaa aattatgtac aaccatatgt
    20521 aatgaaaaat attaagacag atgagggcaa tgtaaaagtt ggaatactag gtcttactat
    20581 aaaagaagta ggggaaaccc ttggtcttga ggattttgaa aaggataaaa aagctagaga
    20641 gcttggagaa caagctgatt acaaaggaaa attatatgca aatgatttag tagaagatgc
    20701 aaagaaatgg ataaaagtta tggaaaaaga aagtccagac ataatagttg cagttgtaca
    20761 ctcaggggaa gaacctaaaa cagatagaaa tccaggaaat agaataaaag agttagcaac
    20821 tacagtggat ggaatagatg caattgtagc tggtcatact catgaaaaaa tagatgaaca
    20881 caaatataaa aataaatctg gagatgaagt aattgttaca caaccaggtt ggcatggaga
    20941 tagagtatca gagattaatt tcaaattaaa taaagagaat ggaaagtggg acataaagga
    21001 aaagtctagt aaattaaaaa taattgataa agaagtaagt atagttgcta ctgcagattt
    21061 acattcacat ttctctgata aattaacagt tgaccttgtt aatgaacgta gtaacccaat
    21121 tgaacctgtt gtagtagatg caggcgactt cttagaccct caaacagatg aaatgactca
    21181 atggtataaa gagtggaaaa gtataagaga taataatagt gataaaacga ttagtagatg
    21241 cccaatggtc atggatatga atcagggaat gtatgatgca gtagtacttg gaaatcatga
    21301 atttgtatct gaaaaagatg aattttggtc agatgaacgc cttttagatg ctgttataga
    21361 ggattttgaa caggtggcaa tttctgtgtt atcggcaaat atatataggc aatctggtaa
    21421 aaattatgtt aaaccatata ttataaaaga tgtggagacg agtgaaggta atgtaaaagt
    21481 aggaatattg ggacttacta taaaagaagt aggtaaaggt cttaaaaatg taaacttaca
    21541 agagcaattt caatataagg ataaattgta tgcaaatgat ttagtggaag atgcaaagaa
    21601 atgggtaaaa gttatgaaaa agaaaaaacc agatataata gttgcagttg tacattcagg
    21661 agaagaacca aaaaatccaa aacatccagg aaatagaata aaagaactag caactacagt
    21721 agatggaata gatgcaattg ttgctagtca tactcatgaa aaaatagatg agcatgaata
    21781 taaaaataag tctggagaaa aagttatagt gacacaacca ggtgagcatg gtaaatatta
    21841 ttctaaaatt acctttaaag taaataaaga aaagggttcg tggattatta atgataaatt
    21901 cagtaaaaca attgaggttg aataacttta taaaatttat agataaagta aattatctga
    21961 tattgacatg atatataaga taaagtttct aatattagta taatgcataa tgtgaaatat
    22021 tttaataata agagaataaa caagacaaat aaaaatattt taacatgtaa gatatagtat
    22081 gaactattat actaatgctt actttataat ttgaacatgt tagaataagt cttatttgaa
    22141 aattttagtg tattagagta agtattattt taccagtaaa aaatattgag aactatatgt
    22201 agatttgctt aaaaaggaat ttacatatag ttcttttcgt ataataaata gtacaatctt
    22261 atatggtaaa gataaagtgt gatattaaat tttattatca tccaaaatct aaggaggagt
    22321 ctcttatgaa aataaaattt attatagtag catcaataat atttttaata gtattacttg
    22381 tggggtgtca aagtactaaa gacacagaaa tcaatgtact cgccacaagt gatttacatt
    22441 caattattcc agataattta atttcctatg tagaagagga gagaaaatat gatgagaatc
    22501 tacttatggt agatgcagga gatttttttg acatgcaaag tggtgagatg aataagtggt
    22561 ttactggtca aaaattagta agatttaaag atgggatacc agagtacaaa aaaatagctg
    22621 aaccacgtga aggagaagtt ccattggcta aaaaaatggc aaagctgaag tatgatgcag
    22681 ttacatttgg gaatcatgaa tttgtatcta atgacaagca gagccttgat aaattggttt
    22741 ctgattttaa aaataacaat ataccattag tatcagcaaa cgtatatgaa caatctggag
    22801 aaaactatgt aaagccatat gtaatgaaaa atataaatac tgacaaggga actgtaaagg
    22861 ttggtatact tggtctgact ataaaagaag taggggaacg ttcaaggttt aaaagttttg
    22921 aacaagataa aaaggctaga gaacttgggg aacaacctca atacaaaggg aaattatatg
    22981 caaatgattt agtagaagat gcaaataagt gggtaaaggt tatggaaaaa aaagaaaagc
    23041 cagatataat agttgccatt gtacattcag gtgaagagcc aaaaaatcct aaaaatccag
    23101 gaaatagaat aaaagaacta gcaacaactg tagagggaat agatgctatt gtagcaggtc
    23161 atacacatga aaaaatagaa cagcatacat ataaaaataa ttctggtgaa gaagtaatag
    23221 taacacagcc tggagctcat ggtaatagta tatcaaagat taattttaag ttggataaga
    23281 agaatgataa gtggtttata aaagacaaat atagtgaatt aaaggttttt ggaaaggaag
    23341 taaatataat tacaacttct ggtctgaatc caaaattttc agatgaaatg gcagttaatt
    23401 tgtttaatga aagagataga gatgttcaac ctatatttat agatactgga gattttttaa
    23461 atactaatac aaaagaaatg actaagtggg ctaaagagtg gcaatacaat gaaaataaag
    23521 gcatctataa aaaaataaat gaatggccaa ttgtatatct tggttatgat gctgtagtac
    23581 ttggtagcca tgaatttaca gaggataaag agacttttga ggaaaaaaaa tctacattgg
    23641 attatattat agaaaacttt gaagaaatga agataccagt attatcagct aatatatatg
    23701 atgaatctgg agaaaattat gtaaaacctt atataattca taatgtagaa actagggaag
    23761 gaaatataaa ggttggagta ttaggtctta ctactactga taaggatgaa gaagacttaa
    23821 gtaactcaag cttaagcaaa gactcaaaga cttcgaaaat tcctgaagaa acaggaaagt
    23881 tatatactaa taatctagta aaagatgcaa gcgaatgggt aaaagttatg caaaaggaaa
    23941 gtcctgatgt gattgttgca gttgtacatt cagataataa tagtactcta aaaaatccaa
    24001 gtagtgaagt caaaaaactt gcaatgtcag tagatggaat agatgctatt gttgtaggtc
    24061 atacgaaaaa taaggtagaa gaacatattt acaaaaataa gtctggggat gaggttatag
    24121 taacacaatc tggagaggat tgttattcaa aaataagtct tgaactaaga aaagaaaatg
    24181 gcaagtggaa tatcataaac aaatatagta aagcaataaa aatgaagtga atttattgat
    24241 atataaacca ttattaatac tgattctaca atataattaa cactgatttt tataatataa
    24301 taaatactga ttctataata taattaacac tactttttat aatgtcatca acactgattc
    24361 tataatataa ttaatactac ttttttaaat ataatcaata ctgattccat aatataatta
//
LOCUS       DAMJYC010000055        24183 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJYC010000055 DAMJYC010000000
VERSION     DAMJYC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.056"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            334..531
                     /locus_tag="PC472_002405"
     CDS             334..531
                     /locus_tag="PC472_002405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727724.1"
                     /translation="MSNLREKIIKELNEFNIKADNDFLEYAVEHIKKFMNEGIADEEL
                     LIRGVVLGASYVVNIENKDLD"
     gene            578..835
                     /locus_tag="PC472_002406"
     CDS             578..835
                     /locus_tag="PC472_002406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727725.1"
                     /translation="MNDYKEKTEPKLNILVQSKVDILCSNSSTMNGVDTSIDIKLSLD
                     NRVIGNLTNEDIKKEISTSFNKCLKEIDKKICSKEVINKLI"
     gene            1006..1707
                     /locus_tag="PC472_002407"
     CDS             1006..1707
                     /locus_tag="PC472_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2727726.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKSDTGDDSKIWV
                     EAIQNIAMKRGVNNG"
     gene            1700..3109
                     /locus_tag="PC472_002408"
     CDS             1700..3109
                     /locus_tag="PC472_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HDF2727727.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKNMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRHERLKYEVIPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSSF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            3100..4602
                     /locus_tag="PC472_002409"
     CDS             3100..4602
                     /locus_tag="PC472_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2727728.1"
                     /translation="MGIISYVKKLFKRPAGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNAKYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLGENKRKAKVKQIITAESRFVEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLNDESNEIEKEINKIKDNNGNG"
     gene            4589..5602
                     /locus_tag="PC472_002410"
     CDS             4589..5602
                     /locus_tag="PC472_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727729.1"
                     /translation="MAMDKSTSELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYI
                     NITRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATS
                     YSKNILINAVETAQITKVTKTMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKK
                     YREDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISY
                     EALRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGK
                     GVYPINEAPVYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDVPLEMWYQEVYGN
                     LNF"
     gene            5622..6956
                     /locus_tag="PC472_002411"
     CDS             5622..6956
                     /locus_tag="PC472_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727730.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFALPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            6972..7355
                     /locus_tag="PC472_002412"
     CDS             6972..7355
                     /locus_tag="PC472_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDF2727731.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            7374..8411
                     /locus_tag="PC472_002413"
     CDS             7374..8411
                     /locus_tag="PC472_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDF2727732.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            8451..8789
                     /locus_tag="PC472_002414"
     CDS             8451..8789
                     /locus_tag="PC472_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727733.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNAEKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            8786..9163
                     /locus_tag="PC472_002415"
     CDS             8786..9163
                     /locus_tag="PC472_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727734.1"
                     /translation="MINIDRRRKDIIRTINMNPTNITITNIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSDDVVLDVNSRDSLEFECIYGRMKI
                     VNVYPQIVKGELCGYQCSLERID"
     gene            9165..9575
                     /locus_tag="PC472_002416"
     CDS             9165..9575
                     /locus_tag="PC472_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727735.1"
                     /translation="MSAFTNAINDINRKKAGMFVLCMSASAMLEGEAKANASWTDRTS
                     HARQSLNAKTLGGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPVK
                     SVQHPGTKAMPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            9576..10397
                     /locus_tag="PC472_002417"
     CDS             9576..10397
                     /locus_tag="PC472_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727736.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVIEILDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDATSDRWVEALSKYTKDLLEIESYKDNWKKNF
                     IAPCALWRTTNVENKRINYHLIEITKTMKCHVVSKNKDEIVKFLEALETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTVVFKMIGKIKREGPIMDKIYSNGNLK"
     gene            10410..10613
                     /locus_tag="PC472_002418"
     CDS             10410..10613
                     /locus_tag="PC472_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727737.1"
                     /translation="MAETINKKTNVSKQEEKYLKNDFLKNSEALGYEKMVVAGALFNC
                     KKEELTKSEFEKLIKDFLEREVK"
     gene            10615..12036
                     /locus_tag="PC472_002419"
     CDS             10615..12036
                     /locus_tag="PC472_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2727738.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSANTGLKGRLAIPVKAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGKQKKGT
                     LTLKDTTENSAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFIFFENTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTILANVVNQALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KSFNDENIVNVGSSAYYENIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            12052..12465
                     /locus_tag="PC472_002420"
     CDS             12052..12465
                     /locus_tag="PC472_002420"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727739.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            12549..13040
                     /locus_tag="PC472_002421"
     CDS             12549..13040
                     /locus_tag="PC472_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727740.1"
                     /translation="MSEIYKRELENEVVEDNDLDEEIEETNEDRLKMKEDEIIAKLLE
                     DSPVPQRTVFLDRLGIPITLKALTEKEISKIRKECTKIVKVQGRREEKLNDDEFTLAL
                     IEKGTVKPNFSNQKLLNAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDDD
                     IKN"
     gene            13217..17491
                     /locus_tag="PC472_002422"
     CDS             13217..17491
                     /locus_tag="PC472_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2727741.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPVPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALRGLTGKIDSFV
                     SGAISKFARLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGS
                     LREKLGGLKDVEKGVAISSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRGFVEDLIKKMPQIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLASTFGTAKLVAFGL
                     TVGIGAIVIAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSSDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLNFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNIGGLKDGATGMLNSLKATGPQALETLKGLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNSLGLEGTQAIDTLRTIFSQASSALDTTNLKQGLSNTFN
                     SFKEGFNSGGIKNAINSMLGTINQAGPQMQQALGKMDGKMGQVFANVDFSTPIETQAS
                     KVLENLNNLGIEGPKALETVRSIFAQASSQIQGSASQTAQQANQEVANALTQGNPTVQ
                     QAGQQLGTDLTNGVVNGVQAGVPVVQQKSNELATATQNGVTNAVNSATPQIDNSNLTS
                     GIDTAFNQATATVQQGASSMYNGAKQSFTQLAQIGREAGSSLYNGATTSFNMLATNVR
                     VACSSMYNGARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPI
                     TASFNVKTTQTTVKRTVNESSGGILGNILDRFADGGVATRTSICGENGAEMVIPLTNT
                     RRSRAIELYEQTGKMLGLGSTPSQKVGNSNIVNNVRQFPLANVLDTDNKEYKEATPNS
                     VNSSNNTINLGGISINVQNSDNKEEMIQELLSQVESGIREALQDIG"
     gene            17573..18316
                     /locus_tag="PC472_002423"
     CDS             17573..18316
                     /locus_tag="PC472_002423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727742.1"
                     /translation="MWQKFKSMNGILKIIIGIIAIILLPITLVLLSIEFLVKAIGSKK
                     KAKIALGCILVFLTTLCTRNVYNVLSIEKEEHIPVKQVSYNDKDKGKSENNIQEQQQK
                     KEESKKLEEQKKNEEKEQNNEPTKFFTDVYLKNKNCVNMTSYEGIKSNLDIFGYKYEV
                     IEPNETTLRQINVYDNQSNDTIQFQFYENDTGEEVVTVMQYNKKDEPDKSVAISDGFH
                     TQPPKFQNHINNTNKEVNSIEEQINFMFN"
     gene            18385..19056
                     /locus_tag="PC472_002424"
     CDS             18385..19056
                     /locus_tag="PC472_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2727743.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVATLEKWVDQAEPLRLIIT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKDTKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            19053..20015
                     /locus_tag="PC472_002425"
     CDS             19053..20015
                     /locus_tag="PC472_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HDF2727744.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            20008..20373
                     /locus_tag="PC472_002426"
     CDS             20008..20373
                     /locus_tag="PC472_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727745.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            20348..20806
                     /locus_tag="PC472_002427"
     CDS             20348..20806
                     /locus_tag="PC472_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2727746.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVEYEFDVLTIDDENIVIDGNIKR"
     gene            20815..21948
                     /locus_tag="PC472_002428"
     CDS             20815..21948
                     /locus_tag="PC472_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2727747.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKATGVITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAESRIVGTIGNVSKGSISVL
                     LGSINGVKSVTNKDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGVG
                     YAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGAL
                     VTVVTPDTLLINVKASFIFSNSFNEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAIV
                     GSMMLTDEGIQDFSNLTINDAKENIKLQDQVVGIGEIVNEVVG"
     gene            21945..22715
                     /locus_tag="PC472_002429"
     CDS             21945..22715
                     /locus_tag="PC472_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2727748.1"
                     /translation="MITSKKGKEMLLTLSPIYEQSLVMNSIYEAIGSEFDNLELLNKE
                     IELQLFPQTATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQMKYIVNPNRLATIIK
                     SYTGTDVYIKENIAPYTFKVTANVDDVINYEDFKYITNKTKPSHLHWMPSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWLLNGSKNLSAYILYNEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGLEMLNGNKLLNAELREEVL"
     gene            22718..23098
                     /locus_tag="PC472_002430"
     CDS             22718..23098
                     /locus_tag="PC472_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2727749.1"
                     /translation="MANAVTTDIARQKMCKARAGDITLPTIVSMAFGDGGIGNDGTII
                     APLSSDIALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKSFGNKYKDSDMEMVFQIDDEF"
     gene            23203..23727
                     /locus_tag="PC472_002431"
     CDS             23203..23727
                     /locus_tag="PC472_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727750.1"
                     /translation="MLDNDKYLNNKIDTTKTELNTRIDTENEKQNIKIDQLIAGGSNV
                     ASTQTITIDDWVEDAESGFKSTVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIE
                     IRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNIEEKLSGSL
                     SNIATSVNELITYC"
     gene            23742..>24183
                     /locus_tag="PC472_002432"
     CDS             23742..>24183
                     /locus_tag="PC472_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727751.1"
                     /translation="MQTEWTFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYIYVGESGYASSSTANNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYSPYTGGAGGGL"
BASE COUNT         9869 a         2705 c         4763 g         6846 t
ORIGIN      
        1 aatatttgac tagggtataa gggattgccc tagtcactac gaacagacta ggcagggcgt
       61 gaggacgctg ttagttcaat tctaactatg ttcaaatatt aatcaacgta tacactaaaa
      121 gtagagaaat tgagggcaaa attttatatt ttgtatctta attcagaagt ctaaaaatcg
      181 ggtggggctt ggtaacctca ctcaccatgc aggtactggt gtttaatcta agttcgattc
      241 ttagaacttg cgacataata tatgtatctc cctatttaaa aaggctaagt gagggtaagc
      301 ttagtctttt atttttttac aggagtatga taaatgagta atttaagaga aaagataata
      361 aaagaactaa atgaatttaa tataaaagca gataatgatt ttttagaata tgcagttgaa
      421 cacattaaaa agtttatgaa tgaaggtata gctgatgaag agttattaat aagaggagtt
      481 gtgcttggag caagttatgt agttaatata gaaaataaag acttagatta atttctaggt
      541 ctttttttta tacaataaat taaaaggaga ataaattatg aatgattata aagaaaaaac
      601 tgaaccaaaa ttaaatattt tagtccagtc taaagttgat atcttatgtt ctaatagctc
      661 tactatgaat ggagtagaca cttcaattga tataaaactt tctttagata atagagttat
      721 tggcaattta actaatgaag acataaaaaa agaaatatca acatctttta ataagtgttt
      781 aaaagaaatt gataaaaaaa tttgtagcaa agaagtaatt aataagctga tttgatttcc
      841 tcaattgaag aaattagttg ttcttgaact ttttctataa ttaattcttt atctaaactt
      901 ccagaataaa aaggttgcaa taaatcaatg taatttaatt agatacatag actctaacaa
      961 gagttctttt ttattcccaa aacgacaaac aaacgaggtg gtggtgtgca agatgtgaaa
     1021 gaaaaagtaa aacaagatta cctaaaaggt atgaaacaaa aggaaatatc agcaaagtat
     1081 gacattagtt taaatacttt aaagtcatgg ataaaaagat acaattgggc taatgagaaa
     1141 aagaagggtg cacctaaaaa taaaagaggt gcacccatag gtaataaaaa tgccactggt
     1201 cctcctggaa ataaaaatgc tgaaaagttt ggtttcttct caaaatatct acctgaagaa
     1261 actagggaat taatacaaga aatatctata aaagataaat ttgatattct ttgggagcag
     1321 ataacaatcc aatacgcagc aataataaga gcacaaaaga taatgtatgt taaagacaag
     1381 gaagaaatga ttaaagaatt aaagaaacat gaaagtacag aaaatggaga gaagatagag
     1441 tatgaatttc aatttgcatg ggataggcaa gcatcttttc ttaatgcaca gagtagagct
     1501 atgagtgaac ttagaagttt aattaaacag tatgatgaaa tgattcataa ggattggaat
     1561 ttggctacag aggagcagaa aaatagagtt gaaaagttaa aatgtgaagt tgataaccta
     1621 aagaaaagtg atactggaga tgattcaaaa atttgggttg aagctataca aaatattgca
     1681 atgaaacgtg gtgttaacaa tggataaagc tttattgaca ctattagatt gttattggga
     1741 taatcctgtt tggtttgcag aggatatgtt aaattttaaa gctgacaagt ggcaatctga
     1801 tgttctgatg gctttagctc aaaccccaaa agtatctatt agaagtggtc aaggagtagg
     1861 taaaactgga ttagaaagca ttgcaactgt atggtattta agcactagac cttttccgaa
     1921 agtagttgct acagctccaa cacgacaaca attatatgac gtactatggg ctgaaatagc
     1981 taaatggcta agtaatagca aggttgagaa gctacttgag tggactaaaa caaaagtgta
     2041 tatgaaaggc tttgaagaaa gatggtgggc tacagctaga acagcagtaa agcccgagaa
     2101 tatgcaaggt tttcatgaag attatatgtt atttgttgtt gatgaagctt cgggagttgc
     2161 tgaccccatt atggaagcta tattgggaac attatcaggt gcagaaaata agcttctttt
     2221 atgcggaaac ccaactagaa cgagtggaac gttttacgat agccataata gagacagaga
     2281 tttatataaa acatttaaag tatcttcttt agacagccct agaacatcaa aagataatat
     2341 tgaaatgcta aaaagaaagt accatgaagg ttctgaccct tggcgtgtca gagtacttgg
     2401 agagtttcca aaaggtgaaa gtgattcttt aatatcttta gaagctgttg aaacaagcac
     2461 aataagagaa gtgaatatat ctaatgacta tatattaaat ataggggcgg atatagcaag
     2521 atatggtgat gatgaaacca taatagctcc aagaataggt gggaaagtat ttgatttatt
     2581 aacttattca aaaaaagata caatggaaac agtaggaaat atattaagag cagttgataa
     2641 atttaaaaat atgtatcatc aaattaacag agtaaaaata aaaacggatg atgatggctt
     2701 aggtgcaggt gtaacagata gattaaaaga agttataaga catgaaagac ttaaatatga
     2761 agttatacct attcaaaatg gttctagtgc tatagaaaaa gataagtact ataataaagc
     2821 ttctgaaatg tgggataaca tgagggagga attagatgca aatttaagta gttttataca
     2881 aaataaagaa gctataatac agcttcctaa tgatgataaa cttattaaac aactatcaaa
     2941 tagaaaatat acagtagatt caaaagggaa aatacaaata gaaagtaaaa aggaaatgaa
     3001 aaaaagaatt ggagaatcac ccgatagagc tgatgcagta atatattcgt ttgcagaaaa
     3061 taacaatact gatttatctt tactgaaagg gggtagtgta tggggataat atcttatgta
     3121 aaaaagctat ttaaaagacc tgcaggagag attatgcgta tgtctagtgg aaacattggc
     3181 gtatataaat tagacgattc tagagttgat tatgagttag caagagaact gtatcaaaat
     3241 aaaaatgcta agtacaagct aggttctagt tttgttagac cgattgtcaa ttcaacaact
     3301 ggttttatgg gtgtacctca ttttcaaata gaagatgaag aagctcaata tatattagat
     3361 gaatttgttt tagataacac atctaaaatg ttaaaaacac atacagatag tttaaagcaa
     3421 ggtgattgtt atatttggat aactagagaa gaaagagaaa atcctttata tcccgataaa
     3481 aaagttagat taatatataa cttcatatca cccgaagaag tgaaagaaat aatattagac
     3541 cctacaacaa aagagcctat agcttatata ttagaaagtc aaaatgaatg gactgactta
     3601 ggagaaaaca agagaaaggc taaggtaaaa caaataataa ctgctgaaag tagatttgtt
     3661 gaggttgaag gtgataagat agaaggttta gaagaagggg aaacgcctaa tgtatggggt
     3721 tttataccaa taatacattt taaaaatgaa gctgatgaaa cattgaaata tgggcaaagt
     3781 gatatagaac caatagaacc tcttttaaaa gcttatcatg atgttatgtt acatgcgtta
     3841 aaaggtagca aaatgcactc tactccaaaa ctaaagttga aattaactga tgttgcaagt
     3901 tttttagcac acaattttgg tgttgaagac ccagttaaat ttgccaaaga aggtggaaag
     3961 ataaatcttg atgggcatga aatactattc ttaaacaaag atgaagaagc tgagtttgta
     4021 gaagtaaaat cagccatagg tgatgctaag gagcttttaa agcttctttt ttattgcata
     4081 gtagatgtat ctgaaacacc cgagtttata tttggagtac atacacctag tgctttagct
     4141 tctgtaaaag aacaaatgcc tattatggta aataagataa gaagaaaaag agaacaattt
     4201 acaaatagct ggcaattact tgcaagaatg gttttaataa tgagttctaa ttctagtggt
     4261 atgaaatatt catcttatga tgtgactata ggttgggatg aagtaaatcc acgagatgat
     4321 aaagaattag ctgaaacact agaaaaagta tgtagtgcat tagataaagc tttagagggt
     4381 ggatttatta gtgaagaatc aacagtaaac tttttagcac agtatataga tacaatgagc
     4441 aattatataa gtgatgaccc tgaaatagtt ggagaaagag aaaagataat aaaaaccaag
     4501 atgttaaaat acagattaga tgactctcaa ggtttaaatg atgagtcaaa tgaaattgag
     4561 aaggaaataa ataaaataaa ggataataat ggcaatggat aaaagtactt cggaattaat
     4621 aactgttgca ggggagtaca agaaatgggc attagaagct agaaaaaaat ttatagattt
     4681 aaggctcaag caagatgatg aaataagaac aatgtatatt aacataacaa gaaatattac
     4741 aaaagaaata agaaaaggaa atctttcaga ctttaacaaa gttaggttaa aacagatact
     4801 aaaacaatta acacaagaaa taaaaatatt aaatgaacaa ctagtattta attttgatga
     4861 atacttaaat aaaaatgttg aaacagctac tagttactct aaaaatattt taattaatgc
     4921 agttgagaca gctcaaataa ctaaagtaac taaaactatg atacaaaaag ctttctatga
     4981 tattaatata agaactgtag aagcttatta tacaagggtt aaggatggtt tatttttatc
     5041 tgatagaatt tggtctaagt gtaagaagta cagagaagat atgaaagtta tattacaaac
     5101 agcagtaaca gaaggtcaag actgtgttaa aacagctaag atgttagaca aatatgtttt
     5161 aaaaggtaag aaaactttag ttgatgaata tccaaatatg ataaaaagaa taggaaatag
     5221 agtacctcaa aatataagtt atgaagcttt aagattggca agaactgaaa tgacatcagc
     5281 ttatggtgat ggggttttag cttctgcaat gattaaccct gcaaccatag gtattcagtt
     5341 tatgttgtcc atggcacatc ctcacacaga tatatgtgac gaaatatgtg gagaggataa
     5401 ttttggtttg ggtaaaggtg tttatcctat aaatgaagct cctgtatatc cattccaccc
     5461 tcattgtttg tgtattatgc ttactgtagt tcaaccatta gatatattag ttggaaggtt
     5521 gaaaaattgg attaaaaatc ctatgaatga tgtacctctt gaaatgtggt atcaagaggt
     5581 gtatggaaat ttgaattttt aaattgaaag gtggtgatta aatgaatgta ataactggag
     5641 aaatggactc aatgaatgcg ttaatatcta gtataaaacc ttctgatatt cctttagcta
     5701 aagatataga catagaagct ttaaaatcta tagatgatga ccctcttgag gtagttgttg
     5761 agataccagc tacaaaatct aaaaggggat ggaattatac tgctaaaagc ttgaaagata
     5821 ttgtagatta cactaatgaa aatactctta atggcttttt aggacatcaa aaagctgaaa
     5881 atatatcaac tgaatttgca ctacctgtaa cgcattggat aggtgcagaa atgaaagggg
     5941 ataaagctta tttcagaggg ctgattgatg ctgatgcaac aaatttaaaa agatggatta
     6001 gaactaaaag gataaaagaa gttagtatat ttggttatcc aaaacttaaa aagagtgcta
     6061 aaggcgaaat gaatgttata ggatatgagc cactatctat tgattggact cctctacata
     6121 gaccaggtat gccaacaagt attgtaggta tggaaatgag tcctaatggc gaacagttag
     6181 atggaacttt tgaagcttta agaatagatt taagagaagc tttaaaagct aagttttcta
     6241 ttaatgataa taattcatat ctctatatac aaaacataag atatgataac aatactgtca
     6301 tatatgagtt ggagcaaaat ggattatgca agctttatag tataccattt actatagttg
     6361 aaaataaaat aaatctaggt gaagaaattg aagtaataaa gaaaataagc tatgaagcta
     6421 aaggagaaat gaaaggagag gaaaacaaat tggaaggaaa agagttaata aaaaatgtca
     6481 aaggattact gcaaactggt gaaatatcat attcagaggt catacaagga ataggcttaa
     6541 ctaaggaaat tgtgacagga gagatggaag atgtaaaaag ttcattaaaa gcagaaaaag
     6601 aattaagaga agtgaaaaaa gtacttggaa tagtaggaga gatggacaca gttgaagtgg
     6661 caaaaaaggc ttcaaaagct ttagaaaatg agaaaaagga agcttggaac tgtatagtta
     6721 ataaagtaat taaagataaa gtgtcaggtg aaatagctca aacattagtt aagaaaatgt
     6781 taaatgttga ggaaggctca agtgaagaag taataacagg agaaatagaa aatatattaa
     6841 atgatgagtt tgtaaaaaat acaatgtcta atatgtataa agataatcca acaacaacag
     6901 gattattaaa ctctagcaat aatggaagtt taacaactaa gaaaaataga atataaagga
     6961 gtgatgttta tatggcattt aaaggtcaac caacgccaag cacaataaca cagataacaa
     7021 gagcaaaaat aagtgatggg aaatctgtaa gagttattct ttcagaaggt gaaagcacta
     7081 aaacacaaca attttatctt ataaatggat tctttggagt cgctatgcaa gacggagaaa
     7141 aaggcgatga agttactttg caaatagagc aagctgaata cgaaacggat aatattgtta
     7201 catcagaagc ttttgaggca gggaaattga tttattggga taatacagct aagaaattta
     7261 ctactacatc tgcaagtaat aggctagttg gtagagtaac agatgggaaa gacagtaata
     7321 atgtaatttg gtttatatta ttacctcaac aatagaaaag gagtgataaa tatatggcat
     7381 ttaaagtaat tagtcaggaa aatttgctgg aacaaaaaag aaaagaaact ttacaagaag
     7441 atataccatt tatagtaaat ggtgaaatgg aatatgtaac aaagaaaata tcaaatggag
     7501 aaatggaaac cttggagtta aataagccac ttggtgaaat gatgactttt agctcgactt
     7561 caaatttaaa agagttatta agaaaagttg tattagatgt tgaactaggc agagagcaag
     7621 tacaactatt atataaacca atctatgaca gtatagcaga ttctaattta ccacaagtta
     7681 tggatgctaa gtgggcttta caaggtaact gtgtattcct agagcatata gaaggtgaag
     7741 aaattaaatt cggtacaata aatgcagaaa atggtccagt tgcaaggata caaacttatg
     7801 caactggttt tgagtataca aaagaaatga aggattttaa ccaaacattt agtgttgaaa
     7861 tattaaataa atcaattggt gagagttaca atgccttgtt aaaccacata catctaagcc
     7921 caataataaa ttttaattat aaagcttcta ataagacagc ttttaaaggt gaaactaatg
     7981 acccaatatg gctaggaatt tggagaacat taacacaagc acaaaaagat acagttatag
     8041 caaaaagaca aggtaatata ttaatggctt ctagtgctga ccaaattgaa atagaaatgg
     8101 cgttaaatgg aggacattta ttaaacggaa gcatgtatcc atctataaaa aatatatcaa
     8161 cagtaattta ttatgatggg tgggaggtta ctgttggtaa aaaaacatat tcttacaaag
     8221 gtgttacacc aggcaaagga tatttgataa gacctaagcg aggatttaaa gagttaataa
     8281 agagagattt aacaacagag gttggaaatg ctgatttaag taagttagta gaaaatcaaa
     8341 ttgtaggtca ttgttataga ggtgcttttg cagcagtaga agaaaatgta caagaaataa
     8401 gttttagata aaacactcat aagagtgtta tttttatgag gtgataatat atgacaccaa
     8461 ctagtaattt aatagaaaaa ttaagactat tattaaatga taaagataaa aaatcattta
     8521 cagatgaaga attaaacttg tttttagaag aagcagactg tatttactgt gcagcttctc
     8581 aaggatgggt attaaaatct ttacaatatg aaaatacagt aggggaaatg tatgagtata
     8641 aagtgggtca agaaacatat aaaagctcta gtataaagga cctagtatct gtagcttatc
     8701 aaaatgcaga gaaattcaag gatatgtgta ctaacaaaaa agaaaaggga agttttatgt
     8761 taggaattag cacagaattt gaaatatgat aaatattgat agaagaagaa aagacataat
     8821 aagaactatt aatatgaacc ctaccaatat tactataact aatattaaaa aaactgaaat
     8881 agatggagct tttgaagaaa ctgaaacaga gataaaatgt gttgttagaa tatttaatga
     8941 aaagacagca gagaaacaaa tatcaagtga aaagcaaggt acttttagtt ctattagaac
     9001 atatggaatg ttagtaagtg atgatgttgt cttagatgtt aacagtagag attctttgga
     9061 gtttgagtgc atatatggga gaatgaaaat agttaatgta tatcctcaaa ttgtaaaagg
     9121 agaactttgt gggtatcaat gttcacttga aaggattgat taaaatgagt gctttcacaa
     9181 atgcaataaa tgatataaat agaaaaaaag caggtatgtt tgtactttgt atgagtgcaa
     9241 gtgcaatgct agaaggtgaa gctaaagcaa atgcaagttg gacagacaga acatcacatg
     9301 caagacaaag tttaaatgct aaaactcttg gaggaggaaa taatttcatt attagattat
     9361 cgcatggtgc agaatatgga ggaatacttg aagaaggttc aaaaccacat gttattactc
     9421 caaaatcagc tcaagcttta tactggagag gtgcttcaca tcctgtaaaa tcagttcaac
     9481 atcctggtac aaaagcaatg cctattataa aaccaactat tgataaaaat ataggcaaaa
     9541 taggtaatat gatttttaga tattggagtg attaaatgag ggcaggaata agaaaagctt
     9601 taatagataa tataaaagaa ttgaaaggtt gttatgaacc taatgtacca aacaaagata
     9661 ctaaaaagcc ttatatggta gttgtacaag ggcaagacaa tgacaatgga gaaacgatag
     9721 gttttgaaag aagtatagaa gtatggattt atgaaggtag aactacattt aagaagttag
     9781 ataaattaac taaacaagtt attgaaatct tagacatgaa tactatagtt gatgaatctg
     9841 aaaatgaagc tttcacttgc atttataaag ggacaagtga aaatgatatt gttgttgagg
     9901 aatgggatgc tatagcaaga ggtataaggt ttagtgtaat agctttagaa gataaagaag
     9961 atgcaactag cgatagatgg gtagaagctt tatcaaagta cacaaaagat ttattagaaa
    10021 ttgagagtta taaagataat tggaagaaaa actttatagc tccatgcgca ttatggcgaa
    10081 ctacaaatgt tgaaaacaaa agaataaatt atcatttgat tgagattacc aaaactatga
    10141 aatgtcatgt tgtaagtaaa aataaggatg aaatagttaa gtttcttgaa gcattagaaa
    10201 caagtttaat aatagataaa agagtaagac ttagagagga taagaatatg tatttaactc
    10261 ttgttagcgt agttgaggat agggaatcag acatgtttac aacaggacaa ttaacagttg
    10321 tgttcaaaat gataggtaag ataaaaagag aaggtcctat tatggataaa atttatagta
    10381 atggaaattt aaaataggag gtgtaagggt tggctgaaac aattaataaa aagactaatg
    10441 taagtaagca ggaagaaaaa tatttgaaaa atgatttctt aaaaaatagt gaagcacttg
    10501 gctacgaaaa aatggtagtt gcaggtgctt tatttaattg taagaaagaa gaacttacaa
    10561 aatcagagtt tgagaaatta ataaaagatt ttttagaaag agaggtgaag taaaatggca
    10621 actggtacat ggaatgaaaa agaaaaaaag gagataccgg gcttttataa cagattcaaa
    10681 acacaagcag aaaagtctgc aaacacagga ttaaagggta gattagcaat accagttaag
    10741 gctaattggg gagaagttgg caaggttgta acaataaaaa atgacttgag acagcttaaa
    10801 actttgtttg gtgatgatat gaactattca gcgtataagt taggtaaatt agctttatta
    10861 ggaaatgtaa aagagctgtt attatatagg cttgtagatg gaaaacaaaa gaagggtaca
    10921 ttaacactaa aagatactac agaaaatagt gcaaaagatg taattaagtt agaaactaag
    10981 tatccaacag ctagaaactt taatgtaaca ataaaatcca atttagtaga ttcagataaa
    11041 aaggacttta tattctttga aaatactaaa cagttattta gttcaagtat taaaggcact
    11101 atagatgaaa tagtactaga aataaactca aatttagata atgagtatgt aattgcaact
    11161 aaagtagctg atagcgatac aattctagca aatgtagtaa atcaagcttt agagggtggg
    11221 aatgatggtt gcacatctat tactaatgag tcttatctaa aagcactaga agaatttgaa
    11281 agatatagtt ttgactcttt tgtacttgat ggtgtggctg atgaagcatt gcaggaaact
    11341 acaaaagctt gggtagctaa aaataaagaa ttaggaaaag atatactact ttttctaggt
    11401 ggaaaaacag aggataatat aaaacagata aatgataaat caaaaagttt caatgatgaa
    11461 aatatagtta acgttggaag ctcagcttat tatgaaaata taaaatatac acctagtgaa
    11521 gtagctgttt acattgctgc tctttctgta agtaaaggta taacgggtag tatatgtaat
    11581 gcaaaaacta tatttgaaga agtagaacca cgattaagtc aatcagaagt taaagagtgt
    11641 ttgaaaagtg gtacattggt cttagatttt gatgatggag atgtgattat agttgatgat
    11701 gtgaacacat ttaaaaaata tgtagatgat aaaaacgaag caatgggata tatctctaat
    11761 atcatgttta ttaatactat aaataaagat acttcattaa aaagaaaaga gtttgtaggt
    11821 aagatattta atgatgcaac aggtcaaaca actgttatat gtgcattgaa gaaatatttt
    11881 gaagaattga tgagtcaagg tattatatca gaatttaatg ttgatataga tacagagctt
    11941 caagcaactg ccaaagcaga tgaattttac tggaagtggg atgctgttaa ggttgatgtc
    12001 atgaaaaaaa tatatggtac tggatactta ggataaagga ggttatagat tatgtataat
    12061 gatgattata tagaagaagc cagttttctg aatggttctg atgtagttat acttattgat
    12121 ggtgtagaag agctatacat ggaagaaata aaagctgatt ttgagcaaga tgagcaaagt
    12181 attaaactgt tagggtgtca aaatgaaata tcaagggttg gtactactaa aggttcattc
    12241 tccttgaatg gatataagac agattcaaaa tttgcaaaat taggatttag gtcttttgaa
    12301 ataatatata atttatctaa ttctgaaaca ttaggatatg aaagtattag attaaagaat
    12361 tgtagattaa aaaaattgcc tcttataaat tctaaagctg gtgaaattgt aaaaatagaa
    12421 gtagagggaa gttttagagg atatgatttg ttaaatgaac tttaaaacaa aataatgttg
    12481 tactttaaag ctatgaacaa ttaattttgt ttgtagcttt ttaaaattaa aaatattgga
    12541 ggaatagtat gtcagagata tataaaagag agttagaaaa tgaagtagta gaagataatg
    12601 atttagatga agaaatagaa gaaacaaatg aagatagatt gaagatgaaa gaggatgaaa
    12661 taatagcaaa gctattggag gattcaccag ttcctcagag aactgtgttt ttagatagat
    12721 taggaatacc aattactttg aaagcattaa cagaaaaaga gattagtaaa ataagaaaag
    12781 aatgcactaa aattgtaaag gtccaagggc gaagagaaga aaaattaaat gatgatgagt
    12841 ttactctagc attaatagaa aaaggaactg taaaacctaa tttttctaat caaaaattac
    12901 ttaatgctat gaaagtaaca aatgctagag aatttataaa gagaaaattc ttagctggag
    12961 aattaagtaa aataagtgac caaatattag aattatccgg tttttatgat gaaataagtg
    13021 atgatgatat aaaaaactaa taaaaatggg gggacgactg actgttttaa ataacatctt
    13081 tgtcaagcat catgttcccc cagatgttta tgttaagaaa aatttaatgt ctcaacgctt
    13141 aatgaaagta tttactcaaa atgaaataga gcaagagaat aaagctatga aaaaataaat
    13201 atctacagaa aggtaggtga gggaaatagc taaaaaggaa atgtatcata ttgatgttgt
    13261 cataagtgca aaaggcgacg gagaaacaaa aagcaaacta agtgctatgg aaaaatacat
    13321 gaagcagaca gaaaaaagga tgcaaacact taataggata aaggttaatc ctgctataaa
    13381 agctactgat aaagcttcaa gtgttgtaaa tagagttaat aacaatatga ataaagcaaa
    13441 aaaaactgtt acagctagaa taaaagctac agataacgcg agtccagtag ccaatagagc
    13501 tagcaataat gttaataaag ctaaaaagac agtaacagca aggttgaaag ctacagataa
    13561 tgcgagttct actgttaaca aagttaataa taagataaaa gaagttgcta agcctgtacc
    13621 tcctgtaatc atacgaggac aagatgaatc tagttctata atagataaag taaaagctaa
    13681 gattcagaat ctaaaagctg atactatcat aaaaataaaa tcacaagctg atgaagctat
    13741 aaatactatt tctcgaacta aaaataaatt acaagaattt gtgagtaaga gatatgaagc
    13801 agcagttaag attagagatg aagctagttc agcacttaga gggcttacag gaaaaataga
    13861 ttcttttgta agtggagcta ttagtaaatt cgctagactg gctactaccg caggagcttt
    13921 aataggtgga attggtgtag gttctgctgt aaaaggattt gctacttttg aacaaagtat
    13981 gaagaataca caagccgtaa gtggagcaac aggaaaagaa atggaagctt taactgcaaa
    14041 agctagacag cttgggagag aaactagttt tacagctaaa gatgcaggag acgcaatgta
    14101 ttacatgggt atggcaggat ggaagtccga gcaaatgata aaagcaattc ctgacgttct
    14161 taacttggca gcagcaggag gaacagactt agcgttaacg agtgacattg tgactgatgg
    14221 actaactgca ttaggaatga ctgcaaatga cacaactgaa tttgttgatg tgatggcagc
    14281 aacaataact aattcaaata caagtgttga gttaatgggt gaaacattta aatacgtagg
    14341 ctctatgggc ggagcattag gagtatctat gaaagattta tctcttgcga caggtctaat
    14401 ggctagtgca agtgtcaagg gaagtatggc aggtacttcg ttaagaggag gtctagttag
    14461 attaataaag ccaccagaag aagccgcatc tgcaattaaa aaatatggaa tagaattaaa
    14521 gaaaaataaa aatggaagtt tagacttggc aggaacaata ggaagtctta gagagaaatt
    14581 aggaggatta aaagatgttg aaaaaggtgt tgcaatatcc tctatatttg gtcgtactgc
    14641 aatggcaggt tgggcggctg tagtaaatgc tagtgaaagt gactttaata agttaactac
    14701 agctattgca gaaagtgaag gagaagctaa gagaattgct gatatgaagt tagatacctt
    14761 atcaggacaa tttgaaattt taaaaagtgc tattgatgat gtaaggataa gtgtaggtca
    14821 aagactagga cctatgacaa gaggatttgt agaagattta attaagaaaa tgccacaaat
    14881 cggtgatgct atagttggag tagtagaaaa gtttgttaat aatttcgata aaataaaagc
    14941 aggatttcaa gtattattgc ctgcaattgg ttccgttata gcctctgtta tggtacttaa
    15001 agccacattt gcttttggag gagccattaa aagtttaagt ttacttgcaa gtacttttgg
    15061 tacggctaaa ttagttgcct tcggattaac agttggaata ggggctattg ttatagcttt
    15121 tgcaggaatg actgttgcta tttctaataa taaaactgct atgatggact tacaaacacg
    15181 ttttggttcg tttggtgagt atgttactac aataatggag actgttggtg gagtcataaa
    15241 gcttacgtta ggtaacttgc ttataatgtt aggtggaatt ggaaaaggaa taggaatact
    15301 tttatctgat aagagctggg atgaaaaagc ttcatcactt aaaaatctat ttggcaaaac
    15361 aacagcagaa attaaaacaa atacaaaaga agctttatca gatataaatg gagaaacttc
    15421 taatgcaaca gctctattaa aaaagtctac ttcaaaagag ttacaaggtg ttactaaagc
    15481 tttctcaaca gcatttgacc aatctaaaaa tgtaacagag aagaaatcta gcgatatagc
    15541 tagagcatta acaaatggat taaagggttt agatgaccaa tctcttacta tgatgagagg
    15601 tttgaatgac aatatggcga taattttatc tggtgtgaca gcagatatga aacctagtga
    15661 caaagttaat aaaatcacca aaaatcttga tgatgctttc aaagcaggta aattaagtgc
    15721 acaagaatac agaagtagca tacaagagac tttaaatttt ataagcaagt atagtgcaga
    15781 ttcttcaaat aatttgaaac aaggtatgag tgatgctttc aatgccttca aggaagggac
    15841 aaatataggc ggattaaaag acggagcaac aggaatgttg aactctttaa aagcaactgg
    15901 tccacaagct ttagaaacct taaaaggttt aggaggaaaa gcaagtgaga ttttcaaagg
    15961 agtggatttt aattcttcta tagatgcaca gaaaactaaa gtattacaga atttaaatag
    16021 tttaggatta gaaggaacac aggctataga tactttaaga actattttct cacaagcatc
    16081 atctgctttg gatactacaa atttaaagca aggtttaagc aatacattta actcttttaa
    16141 agaaggattt aatagtggag gaataaaaaa cgctataaat agtatgcttg gaactattaa
    16201 ccaagcaggt ccacaaatgc aacaagcatt aggcaaaatg gatggaaaga tggggcaagt
    16261 atttgctaat gtagacttta gtactccaat agaaacacaa gctagtaaag ttcttgaaaa
    16321 tttgaataat ctaggaatag aaggaccaaa agctttagaa actgtaagaa gtatatttgc
    16381 tcaagcttca agtcaaatac aaggttcagc ttctcaaacg gcacaacaag cgaatcaaga
    16441 agtggctaat gctttaactc aaggtaaccc aactgtacaa caagcagggc aacaattagg
    16501 tacagatttg accaatggcg tagtaaatgg agtacaggca ggagtgccag ttgtacagca
    16561 aaaaagtaat gaacttgcta cagcaacaca aaacggagta acaaatgctg taaatagtgc
    16621 aactcctcaa atagataatt caaatcttac aagtggtata gatacagcat tcaatcaagc
    16681 tactgcaaca gttcagcaag gggcgagtag tatgtataat ggtgctaaac aaagttttac
    16741 acaactagca caaataggta gagaagcagg ttcaagtctt tataatggtg ctacaacttc
    16801 ttttaatatg ttagcaacaa atgttagagt tgcatgtagt agcatgtaca atggagctag
    16861 aacatctttt acgagtttaa gcagttcagc tataagtgcc atttctgcta tgtgtagttc
    16921 tgtagtaagc caagttagtg ccatgtcgag tcaaattatc agctattgga atagtgtaag
    16981 agcaacagtt tcagcaccaa ttacagcatc ttttaatgtt aagactactc aaacaacagt
    17041 taaaagaacc gtaaatgaaa gtagtggtgg aatactgggt aatatactag accgttttgc
    17101 tgatggtgga gtagcaacaa gaacttctat ctgcggagag aatggagctg aaatggttat
    17161 ccctcttaca aacactagaa gaagtagagc aatagaatta tatgaacaaa caggtaaaat
    17221 gttaggatta ggttcaactc cttctcaaaa agtaggtaat tctaatattg taaataatgt
    17281 tagacaattt cctcttgcaa atgttttaga tacagataat aaagaatata aagaagctac
    17341 accaaacagt gttaattctt ctaataatac tataaattta ggcggaatat ctataaatgt
    17401 tcaaaatagt gataacaaag aagaaatgat acaagaacta ttatctcaag tagaaagtgg
    17461 aataagagaa gcattacaag atattggata atgtcgaatt gttgctaaaa aaatcttcct
    17521 cataaatgat ataatattgt taaaatatgc aaatttatga gggggaaatt ttatgtggca
    17581 gaaatttaaa agtatgaatg gaattttaaa aattattatt ggaattatag ctataattct
    17641 tttaccaata acattggttt tgttatctat tgagttttta gttaaagcta ttggaagtaa
    17701 gaaaaaagct aaaattgcat taggttgcat attagttttt ttgacaacac tttgtactag
    17761 aaatgtttat aatgtactaa gtatagagaa agaagagcat attcctgtga agcaagtttc
    17821 ttataatgat aaagataagg gaaaatccga gaataatatt caagaacaac aacaaaagaa
    17881 agaagaatca aaaaaattgg aagaacaaaa aaagaatgaa gaaaaagagc aaaacaatga
    17941 accaacaaag ttttttactg atgtatattt aaaaaataaa aattgtgtta atatgacatc
    18001 ttatgaaggt ataaagtcaa acctagatat attcggttat aaatatgaag ttatagagcc
    18061 gaatgaaact acattaagac agattaatgt atatgataac caaagtaatg ataccattca
    18121 atttcaattt tatgaaaatg atactggaga agaagttgta acagttatgc aatacaataa
    18181 aaaagatgaa cccgacaaat ctgttgcaat aagtgatggt ttccatacac aaccaccaaa
    18241 atttcagaat catattaata atacaaataa ggaagttaat agcatagaag aacaaataaa
    18301 ttttatgttt aattaatatt tgtataatac tttaaagctc ttacagtttg taggagcttt
    18361 ttattgtaaa aaagaggtga taacttggta atagatattt atctaaaaaa tgaaaaagaa
    18421 aaaatagatt ttcattttcc agtaaatcca caagattctt tatctataaa aaaagaaaaa
    18481 aggtttgaaa ctgtagatat agtaaactta ggtgaatttg atataaaaaa agaaggggag
    18541 aagataagag aaatatcatt taaaacattt ctgcctaact tatatgacgc ttcttattgc
    18601 agatacagcg agttaaaaaa tccaatcgaa gtagtggcaa cgcttgaaaa atgggtagac
    18661 caagccgaac ctttaagact tattataact ggttttggct acaatggatt agtcacaata
    18721 tctagtttta gcaatactca aacagcagga agagaagaag atagagacat tgagataaca
    18781 tttagaactt acagagaact gaagatagag acattaaaaa aagatacaaa aagtaatact
    18841 aaaacagatt taaaagataa tagacctaat acccaaacta aatctaaaat atatactgtt
    18901 aaagcaagtg atacattata taagatagct aaaaatcttt taggtaaggg ttcaaggtgg
    18961 ccagagattt ataatatacc cgaaaacaaa aaagtcattg gtaaaaatcc taatataatt
    19021 aaaaaaggtc aaaagttggt gatacctagt aaatgaaaat aatattaaac gggaaatatg
    19081 atattgcaaa tttcaatgaa ggaataactc tttcagaagc tatagacgga gttgcatata
    19141 agatggatgt atcattagta gaacctaaac aacttaaaga tataaatatt aaaaaaggtg
    19201 ataaaataat tctaattgat atagcatatg agagtaaaaa agaagagaca atatttgatg
    19261 gagttgtatg ggaaactaga aggagtgaaa agagtaagaa actaacattg tcttgcagag
    19321 aaagaacagt ttacatggaa gaatcagagg aacaatatca gtttaaagaa aatacagcaa
    19381 cacagagaat tgaatattac tgtaagcaat ggaatatacc ctattacaac ttagctaata
    19441 cagggaagaa acttgctaaa gtaatacata agacaaatat actagatatg ataaaaaagg
    19501 acttaaaaga aacagcaaca aaaggtggag acttatttag agtaaggatg gataataaat
    19561 taaaattatt caagcttggt actaatgcaa atgtatataa attagatagt atattagaag
    19621 atgctaactt tacaagtagt tttaatgatg cagtaacaag tgtaaaagtt ttaggtaaga
    19681 gtaaagacga aaatacaaaa gcgcctataa ttgggacata taaaaaagat gctgataagt
    19741 ttggaacact acaaaagatt aaacaagatg aaaagataaa aaatgctaaa gaagctaaga
    19801 aagcagcaga agcaatgttc aatagtggag aggaaacaat aagtgtagat tgtgcagtag
    19861 atataaatag aataagagca ggtgacaaag taagtttaaa aagtaaagaa tattatgtta
    19921 tagatgtcac tcatacacta gattctagac cgaaaatgaa gctgaatata gggtctttag
    19981 aatatataag gaggaaattt tataataatg actgatgcta gatttaatgg aattgctaga
    20041 atattgaaag aaaatatgaa taaaagtgta gcaaatggca cttttggaat gggttgtgaa
    20101 cttgcagaaa taacagcaaa tggattaaaa gtaaatggct ataaagatga aatacaggac
    20161 tatctagtat tagagaattt aacattaaaa gaagattatt ttactttttc agatgaagct
    20221 ttaagtggag aatatagaca taagcataaa atagaaactc caaaggaatt gaagccacta
    20281 cgtataggcg ataatgtgct agtagctgtt atgggagctg aatttgtagt aattgggagg
    20341 gttgtaaatg ccaaacctat ttcctcaaag tgaaactttt gaaactgtag aattaaaaaa
    20401 taatgatgaa aatgaattgg acctaaaggg ttctttttta tttgattttg aaaaaggtga
    20461 atttgttaaa aacgcagatg gaacactaaa aaaatgtgat aaggtgcagg cgtacaaaca
    20521 atggtgtcaa aaggctatat taacacctag atacaaaaaa gcagcttata caaacattta
    20581 tggaagtgaa ataaaagact taatagctag taacttatct caaagtgcaa aagagcttga
    20641 aataactaga ttaataaaag aaactatttt ggttcatcct tacacaaaag aagtaggaga
    20701 gtttagcttt aattggttgg agaatagcag gttagtagag tatgaatttg atgtactaac
    20761 aatagatgat gaaaatatag taattgatgg caatataaaa aggtaggtga ttatatggaa
    20821 agagagctac ctataccagt atttttaaca gaagatgagg actctgtaca tgaaaggatg
    20881 ttaagcaact ttcaagatgt ttctacatta gaaggtgact tcatctatga tgcaacaaga
    20941 cctacagcag agcagatagc tgaattaaaa caactaggat tacaaaataa tttaaagata
    21001 gcatttcctc agacttctta tggaacttat ttagagtggc ttggtgaatg taaaggagta
    21061 tttaaaaatc aaccaactaa ggctactgga gttattacat ttacaggtgt acaaggaact
    21121 atcattacaa aaggaactat agtaactact attgcaactg atgaaaaaca gagcatagaa
    21181 tttgagcttc ttgaaaccaa aactatagga gaaaatgaaa cagtagatat taaagcagaa
    21241 agtaggattg taggaactat agggaatgtg tctaaaggta gtatatccgt tttactaggt
    21301 tctattaatg gtgttaaatc agttactaat aaagatttca aaggtggaac agatatagaa
    21361 gatgaagaac attttagaga aagagtcctt gtagcagagc aagaagacaa acttagtgga
    21421 gctagttcag attatataag atgggctaaa gaagtagatg gagttggata tgcttatgta
    21481 gttcccgaat ggaatggagc ggggacagta aaagtattaa tactagataa aaatagaaaa
    21541 gcagcaacac aagagttaat agacaaggtt caagaatata tatatccatt aaatatatca
    21601 gaaggagaaa atagagatgg gaaagctcct atcggtgcat tagttacagt tgtaacacct
    21661 gacacattac ttattaatgt aaaagctagt tttatattta gtaacagttt taatgaagaa
    21721 acagtattaa acaatctaaa aactaagata gataaatatt tagataagat tgatttaggg
    21781 ggaacagttt catacaatgc tatacaagcg atagtaggtt ctatgatgtt gacagatgaa
    21841 ggtatacaag acttttctaa tcttactata aatgatgcaa aagaaaatat aaaattgcaa
    21901 gaccaagtgg tcggaatagg ggaaatagtt aacgaggtgg ttggatgata acttctaaaa
    21961 aaggtaaaga aatgcttcta acattatctc ctatctatga acaatcttta gttatgaact
    22021 ctatatatga agctatagga agcgaatttg ataatctaga attattaaat aaagaaatag
    22081 agttacaatt atttcctcaa actgctacat ggggacttga attttgggaa aatagggtag
    22141 gtttatctac taatatagat gaagatatag aagctagaag aagaaaagtc attgctaagc
    22201 ttcaaatgaa atatattgtt aatcctaaca gactagcaac tataataaaa agctatactg
    22261 gtacagatgt atatataaaa gaaaatatag ctccatacac ttttaaagta actgctaatg
    22321 ttgatgatgt tattaattat gaagatttca aatatataac aaataaaacg aaaccctctc
    22381 atcttcattg gatgccttct tttgcactca aatttacaga tatagaaaaa tttgaggtaa
    22441 agatgattaa tcgaatattt atagatttta gagggaatat aagtaatttc ttagatggca
    22501 tgtggttatt aaatggtagt aaaaacttaa gtgcttatat actttataat gagccaataa
    22561 gcttaaacat gaaaaataag ttatttgtaa aagaaagtga agtatttaca aatcttaaag
    22621 taatcattaa aaagaattta tattatttaa atgggctaga aatgttaaat ggaaataaac
    22681 tgctcaatgc agaattaaga gaggaagtgt tataagtatg gcaaatgcag taacaacaga
    22741 cattgcaaga caaaaaatgt gtaaagctcg agcgggtgat ataacattac ctactatagt
    22801 tagcatggca tttggagatg gaggaattgg gaatgatgga actataatag ctccactttc
    22861 aagtgatata gcgttaaaaa atgaggtgtt tagaaaagat atagaaaatt atgtatatcc
    22921 aatcccaact acttgcagat attcaaccac tctattaaaa aatgaagcag aaggaaaaaa
    22981 cataaatgaa ataggtttaa tagattctga tggcgattta attgcaatta aatcttttgg
    23041 aaataagtat aaagacagtg atatggaaat ggtttttcag atagatgacg agttctagga
    23101 ggtgaataaa tgcctaatga attagatttt aataatgaga ttgaagaata tttaataact
    23161 acaccagctc atgcaaatga gtttaataat cgacaacaaa aattgttaga caatgataaa
    23221 tatttaaata ataaaattga tacaactaaa acagagttaa atactagaat tgacacagaa
    23281 aatgagaaac aaaatattaa aattgaccaa ttaatcgcag gtggttctaa tgtggcatct
    23341 actcaaacaa taacaattga cgattgggtt gaggatgcag aaagtggatt caaatcaact
    23401 gtaacacata gtttattaac acagagaata gttgtaaata ttatagatgc tactacaaaa
    23461 gaaaatgtag ttacaaactt taaaattatc gatgataatt ctatagaaat tagaagtgaa
    23521 acaaggtcag aattaaacgt ttatgtgata aatggaaatg cagaaactca ttttattaat
    23581 gcaactgtag atgataacag agtatctgaa atgactactt attcgtctaa gaaaatcgaa
    23641 gatagatttg ttaatataga agaaaaattg agtgggagtt tatctaacat tgcaacaagt
    23701 gtaaatgagt tgataactta ttgttaaagg agagtgaaaa aatgcagact gaatggactt
    23761 ttgggtataa tggttcgcca caaagtgtta tattgaaacc tggtaaatat aaatttgaat
    23821 gttggggttc ttctggaggt atcaacaatt cttcttggta tactgatgct aaaggtggat
    23881 attccaaggg tgaaattaca ttaaaaaaac aaactacatt atatatttat gtcggcgaaa
    23941 gtggttatgc ttcttcatct acagctaata acactaaaag tggttttaat ggtggtggca
    24001 aaggttattt aaatcaacag gttatgggta cttattattc tatgtatggt ggtggagcta
    24061 ctgatataag gctcgttggt ggtgcttggg ataatgagca aggtttgcta tctcgtataa
    24121 ttgtcgcagg tggtggcggt ggttcatatt ctccttatac tggtggtgca ggaggaggat
    24181 tag
//
LOCUS       DAMJYC010000056        24084 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJYC010000056 DAMJYC010000000
VERSION     DAMJYC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.057"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     regulatory      212..313
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            386..1729
                     /locus_tag="PC472_002433"
     CDS             386..1729
                     /locus_tag="PC472_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2727752.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1908..3272
                     /locus_tag="PC472_002434"
     CDS             1908..3272
                     /locus_tag="PC472_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2727753.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3450..5315)
                     /locus_tag="PC472_002435"
     CDS             complement(3450..5315)
                     /locus_tag="PC472_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2727754.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5551..6768)
                     /locus_tag="PC472_002436"
     CDS             complement(5551..6768)
                     /locus_tag="PC472_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2727755.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6968..7831)
                     /gene="aroE"
                     /locus_tag="PC472_002437"
     CDS             complement(6968..7831)
                     /gene="aroE"
                     /locus_tag="PC472_002437"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2727756.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7913..9835)
                     /locus_tag="PC472_002438"
     CDS             complement(7913..9835)
                     /locus_tag="PC472_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2727757.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9899..10747)
                     /locus_tag="PC472_002439"
     CDS             complement(9899..10747)
                     /locus_tag="PC472_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2727758.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11160..12041
                     /locus_tag="PC472_002440"
     CDS             11160..12041
                     /locus_tag="PC472_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2727759.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12249..13112)
                     /locus_tag="PC472_002441"
     CDS             complement(12249..13112)
                     /locus_tag="PC472_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2727760.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13267..15201)
                     /locus_tag="PC472_002442"
     CDS             complement(13267..15201)
                     /locus_tag="PC472_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2727761.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15313..16326)
                     /gene="galE"
                     /locus_tag="PC472_002443"
     CDS             complement(15313..16326)
                     /gene="galE"
                     /locus_tag="PC472_002443"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2727762.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16350..17219)
                     /gene="galU"
                     /locus_tag="PC472_002444"
     CDS             complement(16350..17219)
                     /gene="galU"
                     /locus_tag="PC472_002444"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2727763.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17417..17737)
                     /locus_tag="PC472_002445"
     CDS             complement(17417..17737)
                     /locus_tag="PC472_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727764.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17727..19718)
                     /locus_tag="PC472_002446"
     CDS             complement(17727..19718)
                     /locus_tag="PC472_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727765.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19841..22384)
                     /locus_tag="PC472_002447"
     CDS             complement(19841..22384)
                     /locus_tag="PC472_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2727766.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22662..22996)
                     /gene="rnpB"
                     /locus_tag="PC472_002448"
     ncRNA           complement(22662..22996)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC472_002448"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23128..23805
                     /gene="srtB"
                     /locus_tag="PC472_002449"
     CDS             23128..23805
                     /gene="srtB"
                     /locus_tag="PC472_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2727767.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23965..24039)
                     /locus_tag="PC472_002450"
     tRNA            complement(23965..24039)
                     /locus_tag="PC472_002450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24005..24007),aa:Thr,seq:tgt)
BASE COUNT         7699 a         4163 c         2712 g         9510 t
ORIGIN      
        1 ttataattat atcacaaact catttataat aaacttccat taatagtata aaacaagtta
       61 gaataattga cattatactc atacatattc aattattgga aaaagtaatt tttttcaata
      121 aataacctta attttttata aaatcgacaa aaaaacgaat aattttatta tatttattgt
      181 aaataataca atattacgat ataatacaac agaaaaacaa atagaaaatg ttcatataat
      241 attagtaata cggtctaata gtttctacaa actcacctta aatgggtttt ctatgaatag
      301 aatgtcaact ctgaatatat gttctattct atctatatga atatatattt ttttgtttat
      361 atcttaaagt tttttggagg taaaaatggt accaaacact aacaatgtac aaccaggtgg
      421 aaataattca tcattaagct ttttagacaa aatgtttaaa tttagcgaac gtggctctag
      481 ccttaaaaca gaagtaatag gtggagttac tacattctta acaatggcat acatagtttt
      541 cgtaaatcca gcaatattat ctgaagctgg aatggataaa gccgccctta taacagttac
      601 catactagct acagccattg gtacacttat atttgcgatt ttaggaaatg caccatttgc
      661 tctagctcct ggtatgggat taaatgcatt tttcacatac tctttggtta taggtgcagg
      721 aatcccatgg caaacaggtc ttggggtagt ttttctatct ggatgtgtct tctttacact
      781 cgcagtaaca ggtcttcgaa agacaattgc agacgcaatt cctagagaat tggcagtagc
      841 atcttcagca ggtataggtc ttttccttgc ttttgttggt ttaaaaaata tgggtgttat
      901 aatagctaat ccagatacta tagtttcact tgcagatttt acaccaacag ttttaatagc
      961 cctttttgct cttatcttaa tggcaatact agaatataaa aaagttaaag gtggaatatt
     1021 gataagtata gtagttgcaa caataatatc tgttatattt ggatttgtgg agttgccaac
     1081 agcaataatc tcaacacctc ctagtatagc accagttgct tttaaattag atattgtagg
     1141 tgcattgaaa ttctcattaa ttggacctat attctctttc atgtttatag acatgtttga
     1201 ttcattagca tttcttatta cttgttgtaa acaaatgggt cttgaagatg agaatggtga
     1261 aataaaagat ttaggaagaa tgttatatgc agatgtttct tctactttaa taggctcatt
     1321 cttaggaact agtactgtta caacatttgg agaatctgct tctggtattg cagcaggtgc
     1381 aagaacagga cttgcttcaa tagtaacagc agcattattc ttagctacac ttatatttac
     1441 accattactt ggtatagttc ctacttacgc tgcagctcct gcacttgtaa tggttggtgt
     1501 gtttatgttt gaaagcataa aacagattga ttttgctgat agcaaaatag ctgtacctgc
     1561 ttttgttaca gttttattga tgcctttaac ttatagtata agtattggtt tatgctttgg
     1621 atttatttca tatataataa tgcatgtagt agctaaagaa actgataaaa tcagtattac
     1681 actttggatt ataggtttac ttgcagttgt aaacttagtt ctaagttaat atttttaaat
     1741 agttctaggt caatatttta aattatatag aataaaatct tatttatttt aaatttataa
     1801 atagcaaaat cactttatat gtaaattgat tttgctatta actttgtata gaaaatttat
     1861 tatcataata tatagatata aaaatatata gaaaaggagt ggtgtttatg tcacaattac
     1921 ttataaaaaa tgcatatcta attactatga atgcagatag agaggtatat gaaaatggaa
     1981 gcatacttat tgaaaataat attataaaag aggttggtaa gtttgatatt aatattgtag
     2041 ataaagatgc agagatatat gatgctaaag gaaaaatact aatgccaggg ctagtcaata
     2101 ctcatgttca cttatctcaa caattaggac gtggtcttgc agatgatgtt gtacttttaa
     2161 cttggcttag agaaagaatt tggccatacg aaagtagctt caattatgaa gattccttga
     2221 tttcatctac tgcatgttgt gttgaattga taaaatctgg tgtcactaca tttttagaat
     2281 ctggtggtca atatgtagat gctatggttg aagctgttga taaaactgga cttagagcct
     2341 gtttagctaa atcagttatg gatacaggag atggtcttcc tgaggcatgg caaaaaacta
     2401 ctgatgagga aatcaacact caattagact tattccataa gtttaataat acatctaatg
     2461 agagaattaa aatatggttt gcattaagaa ctatttttaa tacaactgaa gaattacttg
     2521 ttagaactaa agaactagct gataaataca atacaggtat acatatgcat attgctgaaa
     2581 tagctgatga aatttcattt gtaaagcaaa acaatggagt tggaacagtt gaataccttg
     2641 ataaactagg tgtattagga ccaaatcttt tagctgctca tacagtttgg cttactcata
     2701 aggaaattga tttatttaga ttacatgatg taaaagtttc tcatgaccca ggttcagcta
     2761 tgaaagtggt tctagggttt gcatcaattc ctgaaatgtt agaaaaagga atacctgttt
     2821 caattggtac tgatggagct ccttcaaata atagaatgga ccttatgagg gacatgtatc
     2881 ttacatcact aatacataaa ggaagaactt taaacccaac tgtcgtacca gctgaagaag
     2941 tcttagaaat ggctactata aatggagcaa aatgtgcttt actagaaaaa gaaattggta
     3001 gtcttgaagt aggaaaaaaa gctgatatga ttattctaaa tccagataca attcattgtt
     3061 taccaatgca taatccaatt ggaaatatag tctattctat gactagtgaa aatgtagact
     3121 ctactatttg tgatggtaaa tggttgatga aggaaagaaa agtattagtt gtagatgaat
     3181 ctgaattatt aaataaagtt aaaaaacaag cttctaaaat aaaagataaa gcagaagtta
     3241 aaattccatc taaatttaaa attataaaat aacataaatt tcaattgttt tgtaaatatg
     3301 aatatataaa tatatttata tacatcatat atacaaaaca attgtttttt tattttatat
     3361 tcattagcat tgctcataaa atacaccata attccagaaa aaataaccaa tcagttagga
     3421 ttacatcatc taagcaatat ataaacgtct tagtttataa gttttattct actcttatct
     3481 aaaaccatct ttaaagagtt ttcaactgtt tcaattacat ctttataact caagtacaca
     3541 tttgcgtaga tacaatcttc atataaatta tattcagtta ctctacaata cagcagtttt
     3601 cctccatcta ataaattgct caaaatatag ttttcttttc ttgggacata gcctatcaca
     3661 tatccagttt caccaactat tttaatagct ttttcatcat atagattatc tttctctcta
     3721 gttaaatagt atatcttact tttctccaat tctttttcaa gatattctct atttctaaac
     3781 cttgaaccag caacagctat tgaagtcaaa tagatatcct taccatgttc atctactttt
     3841 attttttcaa tatttacaaa ttcttttttt aaattattac tttcatataa tactcttctt
     3901 aattcatcct tcaatataac atttttttct ttttcatacg cttctttaac aatttttaat
     3961 tcttcaagag atagtttctc tctgtatctt tttaagatat ttatggattc ttttcttact
     4021 tcatttatat ttccaaatat tccttttaaa gatatattct ttccttcagg atatagtgat
     4081 ttacttcctt taagcacaat aaaaaataac atatcatcat aatattcttg agtcaattta
     4141 tctttttcta tatctttcat ttttccaatt aaatcatcta ttttaaacgt ctcttcaaaa
     4201 aaatttaata attttaatct agaacttgtc gttccctttt tagatatatg ccaatatacc
     4261 tcaaaatctc taatatctct atttagatat ggcaacaagt catcaaaaga taagtttaca
     4321 tcataaattt ctgacatttt tataatatct tctgtttttc cactagcatc tcttaatgct
     4381 tcaaaatata tatttttcca tttttcactg agcaagatac catttatttc tttttcaaat
     4441 tcaatcttat tgcactctat attttcatct tttaagacgt ttatagctat taggaaaatt
     4501 gaatataaac tttcaaagtt agtcccataa tttactacag taggtaaaaa tcgattcact
     4561 aattctaatt tattacctat atatttaaat tctacactta gcaagtaatt gcatataaga
     4621 cgtgctaaat tatttatctt ttctttatct aaatctcgtt tttcaagata ttcattcaaa
     4681 tctacaattg aaataatata gttcatcaat aatcgttcgt actttgtatc cttataacca
     4741 tcttcaatta gataggaatt tattttagaa tctaaatttt ccatattaac aatagcaaat
     4801 acttttattg aacctgtagc ctttttagat aaattaaata aatatgtatt gtaaaattca
     4861 agttttttaa tagtattact cagataaaat acgtattctc cagatttaga aaatgtatct
     4921 acaacttctc ttaagttttc tacatttaga tatttctcac ttaaaactaa ccctagtttt
     4981 acatactcaa cttttttact cttatatatt aattctttag ttaatttata taaccatttc
     5041 tcatcaataa tcatttcttt taccaaagta tttatctttt tcataaaaat ttcgaaataa
     5101 caaattaaag acttagaatt actaccctcc aataaataaa tttcattaga ttcactttta
     5161 tacatattta aaatttcatc tactagatta tttgcaagct ctacttgttc tcttaacggt
     5221 atactttcat atatacatag gtaattcatt tcaaatgatt ctttgtatcc atcataacca
     5281 ttaaattcct gcaatcgctt aaaaacattc tccaccaaat tatcctcctt tacatgatgt
     5341 aatttatatt gattatacta cttatttcat caagtttaca tttatacgca aaaaaatatt
     5401 actagaaatt gaaattctag taatatcctt attattaatt atgtaaatac cctatcttat
     5461 aactgtaaat aatatcctta ttaattttaa ttacttttaa taaaaatcta tcctagattt
     5521 agacttctct atgcatatat tatactaata ttagacatca taatcataca ctttttcttt
     5581 ttcatatctc atgtttacaa acaatgcaag taaaattcca acaaatgtta ttgccacatt
     5641 aaacaataca acatattttg gtccattaac tccccctgat tttgtaataa tacctgctat
     5701 atttaaaata acatagtttg ctatactaga tgcaatcata actatagatg ttatttttcc
     5761 tttattagtt ggaaacattt catttgcagt tgacgttgtt aactgaagaa cacctcctgc
     5821 tgctgaataa cctattacaa atccccctaa catacaaata gttggagttt gaacaaaata
     5881 tacaactaat agcataagta aagctattat aggatatata actaatattc ttattggttt
     5941 aatatatttt ttaataagta aagaagttat taatactgca catattactc ccattgagta
     6001 aaaggattga atttttgtag ggtcagccat cccatacaat ttacccaatt cctgattaca
     6061 atttaaccat aatacaaatg tagatgtaca tgtaaatcca atacaaacaa gagctatact
     6121 tgttggtgta aatttcatct tctctgattt aactgttttg cctttgttat caataacatt
     6181 attagctggt ggaaatggta acacagctat caaaaaggca tctactacaa taagaatcgc
     6241 tgttactata aaaataactt taaatgatat tgaatttgct gcaacaaatc caattataaa
     6301 aggaagcaaa aattgtgcta aagctattgt aaatttagta aacatatttg ctattgttcc
     6361 taaactagca aatatctcca tacaagaagg agttacacag gtatctaaga atgaatttgc
     6421 tgcccctcca attattgcaa atacatatgc tatataaaaa tttggtgaaa atacaattcc
     6481 aacaaagaaa attgcatgta gaaagtttcc tataagacca cttacttttc ttccatactt
     6541 atctgaaata ggtcctgcaa atggataaga tataagtctc cctaatccta gtgcagcaat
     6601 aacagctaaa acaatactta catccaatgt tccatctgat aatgttttag ccccccacat
     6661 tgatgaaaat tctggtttgt attgtcctaa aattgaaatt ccaataccaa gtaaaaaata
     6721 tgaaaaatac aacgctatgg cagtaggata atatttttta ttgttcataa tttttctccc
     6781 attcttgtta atttattaac atttttgtgt acaataaact catccccata atggatttaa
     6841 agtatagtca gttaaagctt caaatttaac tggactatga ggcaaaaccc actatgacaa
     6901 tttgtaattt attattaaaa atattgaaat acgtatgttt tttcattgtt taagatatta
     6961 ctcttatcta tttaaagcat aattcattaa cttcttctac aggcatttct aatccagtat
     7021 atagattaaa agcttctgct ccttggtata ataacattcc taatccacca atagccttac
     7081 atccattagc ttcagcatct tcaatcattt ttgttttctt aggattataa acgacatctg
     7141 ttactactaa atcttttctt aaaacactag tatcttttat atttgtttca ttgtcataag
     7201 gcttcatacc aataagagtt gcatttgtca atatatcact tgattgtatc tcctcataaa
     7261 gtttgtttgt atcttctaaa tcatatagat taacaacaca ttctggtaca tcttttttta
     7321 tattctcaac tgtttgctct gctcttttat aaaaatcatc cttaggatta aagatagata
     7381 tttctcttgc tccatctaaa gcacattgta cttgtatagc agttgctgca ccaccagcac
     7441 ccataattgt aatcttttta cctttaactt ctaccccatt ttcttttaaa ttacgaacat
     7501 agcctactcc atctgttata tgaccaacta actttccatt gtcatttaca attgtattac
     7561 atgctccaat tatacgagct gctggagaaa gttcatccat atattctgta acagcagatt
     7621 tacatggcat agtaacattg gctcctttta tattaaaagt ttttattgct aaaagtgcct
     7681 cttttacctt atctactgtg atatcaaagg ctaaatatct ataatctaaa tctaatttct
     7741 taaaactata attatacatc actggtgatt tagagtgacc aaccggtgta cctattaatg
     7801 caaataatcc tgttctacct gaaatttcca taactataat ctcctctcaa tattatcatt
     7861 ttctttgcaa ataacctatt cagttattta caaagaaaaa tttatttatt atttatatat
     7921 ttaatgctac ttcaaaagct tcttttgttg catctaatgc cctacgagct tttactgcat
     7981 caccaacaat ataagtttct tttacaactt cttttataac ttcacttaat gggtcgtaat
     8041 tacgagaacc catagcaagt actactgaat caaaaccttt taaatcatgc tctgtgccat
     8101 ctgtaagtaa atagtcgaca ccatcatcat aaaatttaga aacttttgca ccagtaatgg
     8161 tatgtatatt ataattttca aaatcattca taaggaattt acggtgttct gaaattacat
     8221 ctgcaccaac ttcttctcta agctcaataa cagtaacctc atgttcttgt tctccaagga
     8281 aggctgccac ttcacatcca accattccac ctccaacaac aagtactttt ttcccacaag
     8341 attttttacc atcaagaagg tctacagcat gaattaatcc tgaatcattg attcctggta
     8401 taggaagtac aagtggagtt gctccagtag ctataataac tacatcaggt gatttctcct
     8461 ttataagttc tactgtagct tctgtattag tacatatttt tacaccatat tgattacatt
     8521 ttgaaatata acttctaact aagttagtaa tatctccttt ccctggagga tatgcagcta
     8581 aacgcatttg cccaccaaga acttcagatt tttcaactaa agttacatta tgtcctcttt
     8641 tagcacatac ccatgctgca agcatacctc caacacctcc accaacaaca agaacttctt
     8701 tgctaatttc agctggctta agctctgctt catgtcctaa tataggattt gcaagacagg
     8761 ctataggttt gccttggaac atatttggta cacatccttg taagcatgca atacatggta
     8821 tcatttcgtc tagttttcca tttttagctt tattaggcat ttctgggtca gccaagcttt
     8881 gtctaccgaa tgctactaaa tcacatcttc cttgtctgac cattaactca gcaaactgtg
     8941 gctctgtata acgtccaact gttattactg gtatactaac agcttttttt atttcagtaa
     9001 ctaattctgc actgaatcca gcatgtaaag tcgtaggagc ccacatatat tcatcatgta
     9061 tatgtatact acgtgaaaca tgaagtccat ctattccaca atcttctaga tatgctgcta
     9121 ttactgaact atcgtgtaca tcaattcctc ctggaacatc atcagtgctg tttatacgac
     9181 ataatattgc tagagaattt cctacttttt tacgtatatt ttcaatgata agacgaggta
     9241 atctcattct attttcaaaa catccaccaa actcatctac acgcttattt gttctttgtg
     9301 atataaaact actaaccaga tatccatgag cgcagtgaac ttcaacagca tctgcaccag
     9361 ctttttgtgc acgtaatgca gcatctccat aagattctat aagttcatat atttcttctg
     9421 ttgttattgc tagtggtgta ttacgaccat ttgatgcagg tattgcagaa gcagctttta
     9481 atggatgtcc tgctactttt tcatttcctt cagaaccagc atgttgtaat tgtatagata
     9541 ccttcgaacc tgcatcatga caagcatcta ttacattttt aaaactatct attgtgctat
     9601 catcatataa acaaggctta cgaggacctc cttttgcttt tttgtctaca acactagatt
     9661 ctatagttat taatccaaaa ccacctaaag cacgctcaag ataataagcc ttagaagttt
     9721 cacttaaagt accatctgtg tttgcaaaat tgtttcccat agggggaact acaaaacgat
     9781 ttggaactgt cattgaacca attcttatag gtgtaaacat agaactaatc ttcataaggt
     9841 tttccttcct ttcaagtttt atattataat actctgtata tttttatatt atataagttt
     9901 attttggaga tacttcagac catgctttat ccaaaacttt tttagttgca ttaaatacag
     9961 tttcagcagc agtggataac cctttagata ttagagaatc agatattact tctgccccta
    10021 tgactcttgg tgatatattg tgttctttta ttattttcac aaattcctct gtgtttccat
    10081 atccttcacc tggagccaaa cggtcatgta gtgattcatc cctaagtttt tcataaggag
    10141 tttctaaaac atcacacact tgaattgata ctatcttatc tgctggaaca ggtgcaaggt
    10201 cctctgctgt ctgctttgct ctagcccagt gccaagtatc taatataagc attgcatttt
    10261 cacgattaca tcctttcaca acacgccatg cagtagccaa atctggtaca ccactatatg
    10321 gcataaactc aagacctata ataagttcac ctgctctgtc acataattct cccaatgcag
    10381 ttataatttg ttcttctggt atcatctcta ataatccaca atttatatgt ttaacattaa
    10441 ataagtgagc catatgataa acattttgct ctttttctct ttgagcttcc gttctatctt
    10501 cttctcttcc ccattgagtt atatattcta cttctgtaac tttcatatca tattcattaa
    10561 gaattgcaat catttcttca tctgtaacac ctgcattttt tgcatcaata taattttcag
    10621 cacgcaatcc aataccatca aacccagctt cttttgcaat acgaactcta tcacggaatg
    10681 aaacttctgt tcccaatgta taagaactaa ttgtaattgg aatattaaga tttttttgat
    10741 tattcatagc ttaaatttgc cccctttatt ttctttataa ttaatagtat ttttgatttt
    10801 atatttttat ttatcctact agtagtatta ttttacctta aaaataaaag tattttttga
    10861 tttctattta tctagatttt ataaatttat tgtatcacga gactttacta ataacaaatt
    10921 gataatttcc tattaattca tagattttgt ttatagattt tatttataag ataattcttg
    10981 tatttttcta ttaggtcgta taaatatatt tatcttataa ttcagacaaa aaacgataaa
    11041 tttttttaaa agcttttatt cttactctat aatcatatat aataaaaatg taaaaatatc
    11101 ttaaaggtta acttctacaa atatataaat aaaaatacct atgaaaggat gaacaaaaat
    11161 tgaatttata tcacttacgc tattttgtta cattggcaca tttagaacac tatacaaaag
    11221 cggcagaaaa tttatctata acacaaccaa gcttaagtca tgctatttct ttactagaaa
    11281 atgaacttgg ggtggctcta tttgaaaaag aaggtagaaa tatagtcctt actaagtatg
    11341 gaaaaatatt tttaaaagat gtggaaaaat cattagaaat tttagattca agtgtaaaat
    11401 cattaaaaat aactggtact ggtgaaggac aaatagactt agcttttctt agaacacttg
    11461 gtactgattt tatcccagac atagtgcaca aatttttaaa atctaatcct gctaaatcaa
    11521 tagattttaa atttcatact ggtgtaacta ctgacattat tcaaggtctt aaagaaagaa
    11581 aatatgatat tgcattttgt tcgaaacttg aaaaagaaaa gggaattgaa tttatacctg
    11641 ttgccaaaca agatttagta ctaattgtac catatagtca tccactagct gcaaaggata
    11701 ctatagattt aaaagaaacc ataccttatc ctcaaatagt atttaatcag agaagtggtc
    11761 ttagatatat aattgatgat atgtttaaaa aaataaatca acagcctaat atagtctatg
    11821 aagtagagga agaccaagta attgctggat tagttgcaaa aaatttcgga attgctgtag
    11881 tcccaaatat gaatatgctt agctttacaa aagtaaaagt aattcagatt attcacccaa
    11941 gttgggaaag aaacttttac ttagcattta taaaagatag atatttgcct cctgcaatta
    12001 aaaactttaa aaactttgtc attaaaaacg ctcaattata atttattaat aaaggggtta
    12061 tttttaaata tcctctttta ttctttttat aaaataaaaa aatctatact tttaaaagta
    12121 tagattccta actgacagtc ggcatccgag tctcagtaat tatcgttata caacagcaac
    12181 tggattcagt tcctgaataa ctaggttact tacattatat cagatttttt aaaatttgtc
    12241 aattgtatct aacgcatttt ttcaagccat ggtccaacag gctctacaat aggaacttct
    12301 ttaaatcccc aatttcttat atcattcaca ctcgttggag tatatatttt gtttctgaca
    12361 gatttgaata ttttgttttt ttctccatat tgcatacact ttggattagc ttctattaac
    12421 cctcttcttg ctctgctaag aggattacat aaaatatctg ctatttctaa accttctcta
    12481 tagtcagcat cttctttttt ggcaattata aaacccttaa ccttatctct taattcttca
    12541 tctatatcta agtatattgt tccattatta aatacatcaa aaaacgcctt ttgtactttt
    12601 aagtttgaga catcatccct tgactctata actattcgtg cagattctac agtttcatta
    12661 tttacaaatg aataaaaatt ctgaagaaca tgtataaaag ccacaacata cggttccttt
    12721 gaaggtgcat aatatttctt taatctagct ttatctactg ttactgatat tatagtacac
    12781 tctaatctag atagaaataa aggcaactca tcatagaata acttaatctg tgaatcatca
    12841 aacttctcaa atgctcttga ttttcttgat atatctgtca aatgtattcc tatgttactg
    12901 gttccaaaac aatactcttt aaatgcatcc atttctttcc tcaaagaaca tacataatcg
    12961 ccttgactta ctgcatattt atattcaaaa attactcctg tcaatgtaaa tgggtcttca
    13021 tgatttatat cagctcttcc actttcatca ataaacatta tatatttttt ctttataatc
    13081 ttactcaccc tcttaacata tgatgatagc atctatattc attttatatg agttttaaaa
    13141 atattccaag atttttatat aacttaaact taattttata ctaaaaaatt gagatgtaaa
    13201 acaatcaagc ttttattgat tgaatttcac atctcaatta taattatttt tcaatatata
    13261 tttttactat tttgcaagaa tatccattaa tttatccatt actatataag atactccgcc
    13321 tcctatttga taaggagttt caactttttt atttgcaaat actctttgtt gctctgatga
    13381 taatcctgaa ttatccatta aaactattgg tgaatgtaat tttacagcta atggtccagc
    13441 tgataaagca tctactaaag catctgattt actaactaat acagcctgta aatcagtatc
    13501 tccatagaat ttttctatca ctttggcatt agtatctact ctactatctc catatattct
    13561 atttcctgaa atatcttcag tagttatatc attcattttt gctattactg aatcgttaat
    13621 tgcacttggt ccacctataa agtatgcatt ttctaatttt ctttctgtta gccatttata
    13681 agattcttct gtaattccat ctttattgac taatattata ggttgttttt cttccccagc
    13741 tttagaagct acagataaag catctgcctc cccatttcca gaaaccatat atgcagtttt
    13801 tatttcttct cgatttccaa gttcttcagc tattcttgta gcagtttgat atctatcttg
    13861 acctgctatt ctctttgttt ctgtgttgaa agtagttttt atttcttgct ctaatttaga
    13921 acttattgca ttttctccac ctatcaagat tactgtttta ggtgctaata acttcatttc
    13981 ttcaacagtt tcagttggta aattagctgt attagtaagt agtattgttg agtcatagct
    14041 acttgctaaa ggagttgctg ttataccatc agctattgca ttaccattta ctaatattac
    14101 agtatctgaa ctatcagcat tccagtttcc aactgaactt atctttttag ctgtatcaaa
    14161 tctatttgca cctataattg tatcccatgt aggatttata gttccatctt cattaaaagt
    14221 ataatctttc ccatctattt tttgagaacc agttagcatc tttccatctt tgtctaaata
    14281 gtaagactta ccatctattt gttgccatcc tgtctgcatt attcctgttt tgtcaaagta
    14341 atacttatta ccatctattt cttgccatcc tgttactgct aaaccatcag atcctagata
    14401 gtatttcttt ccattctttt cttgccatcc tgtttgcatt actccatctg agttaaagta
    14461 ataagttttt ccgtctatta ctttccatcc agatactctt gctccatcag ttcctaaata
    14521 atattttttg ttattaattg ttttccatcc tgtttgcatt actcctgaag catcaaagta
    14581 atacattttt ccatttactt ttagccaact attcttttgg tatgttccat cagattttac
    14641 ataatacttc ttaccatttt ctgttttcca tcctgtttga tttagattta tcttatattt
    14701 tgccgctatc tttacatagc tgtcgttact ataatctata attacatctg aacctacagg
    14761 aacttggtca aataaccatc taacatcttt attgtgcatt cttatacatc caccagaaat
    14821 atgctttcct atagaactct cattattatt tccatgtatt ccatatgtat ctccataagt
    14881 tccttttaat gctaatccca tccatctatc tcctaatgga tttcttggac ttcctccagg
    14941 tatattacct ttataatatg gtctattttt tatcttgtta actacttttg ttttccctgt
    15001 tggtgtttct gacccctttt tacctgttgc aactctaaat tcttttacaa gtttattatt
    15061 tacaaaatat cctagagtat tttttcttga atttactata atcacatgtt tactggctgc
    15121 gcttgattct ggtacgccac atgcaaatac taaaactaaa gtcagtgcta gtactaaata
    15181 cttttttaga ctttttttca ctataacttc cccctctttt agaaacatta ctacatgtgt
    15241 ctaattataa cataaaatta ccaatacctt atatttacaa atttgttaca tttatacata
    15301 taagtatgta ttctactctt catacccatt aggattcata ctttgccatc tccatgaatc
    15361 ttcacacatc tcttcaagtt catacttagc tttccatcca agctcttttt ctgcctttga
    15421 agaatctgca taacacatgg ctacatctcc agcccttctt cctacaatct tatatggtat
    15481 ttcttttcca cttgctttag aaaatgcttt tactaaatca agaacactat acccctttcc
    15541 agttcctaaa ttataaacaa caagtcctgg attttcttct aatttttgta atgcttttac
    15601 atgaccagca gctaaatcta atacatgtat atagtctcta actccagttc catcatgtgt
    15661 tggatagtca ttaccataaa cactcaattc cttcaattta cctactgcta tttttgttat
    15721 ataaggcatt agattgctcg gaactccatt tggttcttca cctattctac cacttttatg
    15781 agcaccgact ggattaaagt atcttaaaag agctatatcc aatgacttat ctgcttttga
    15841 tatatctact aacatctgct ctatcataag tttagttctt ccatatggat tggttacaga
    15901 aagtggaaaa tcctctaata taggacaagt atgtgggtct ccatatacag tagctgaaga
    15961 actaaataca aactttttaa caccatattc tctcattaat tcaaataaat ttaaggtact
    16021 tattagatta ttgctgtaat attcaattgg tttttctact gattcaccaa ctgcttttaa
    16081 agctgcaaag tgtataattg attcaatatt attttcttta aatacaacat gcatttcatc
    16141 tttatttctg atatcaatat tataaaattt tactggtttt ttagataatt ccttgattct
    16201 atcaaccact attgagttgc tattacttaa gttatcaact ataacaactt cataacctga
    16261 ttctaaaagt tctattgctg tatggcttcc tatatatcct gctcctccag ctactaaaac
    16321 tgccattgta attacctctt ttctaatatt tatttttcac ttactttttt tctcatatag
    16381 tccattaaat catcttttaa attttctctc tttagagcaa agtctattgt tgcctctaaa
    16441 aaaccaattt tatctcccac atcatatctt ctaccttcaa agttataagc ataaatagcc
    16501 tcttgttttc caagagtttg aagtgcatct gtcagttgta tctctccacc tttaccaggt
    16561 gtctgtttct ctaatatgct aaatattgca ggagttatta tatatcttcc aagtatagct
    16621 atatcagatg gagcttcttc aattgcaggt ttttcaacca tatctttaac tttatatact
    16681 ctatcttcga tatgttttac atccaatata ccatatttat tagtatcttc tttagctatc
    16741 ttttgaacac ctaatacagt tgttttatac tcattataag tatctattaa ttgttttaag
    16801 cacggaacat ctgaatctac tatatcatca cctaataata cggcaaaagg ttcatcacct
    16861 ataaaacttt ttgcacagta aattgcatgt ccaagacctt ttggctcttt ttgtcttata
    16921 taatgaatat ttaccatatt tgatatgtct cttaccattt ctaacatctc tgtcttacct
    16981 ttttgctcta gttctaactc taattcaact gatttatcaa aatgatcttc tatagatttt
    17041 ttatttctac ctgttattat aagaatttct tcaattcccg attctatagc ttcttctatt
    17101 atatactgta aagttggttt atctactata ggaagcattt ctttgggctg tgattttgtt
    17161 gctggtaaaa atctagttcc tagccctgct gctggtatta ttgctttttt aactgtcatc
    17221 tgcatttttt cacccttctt cgtaaatttt ctattgtacc aaattaatat accatattta
    17281 tactttttct tcaaactcta tattatccca atatttaatg ctataagata atataaggta
    17341 gatgaaattt catctacctt tagttttata agttaatatt gtattgttta tattttatcc
    17401 aaaacttata caggtactaa atttcatcta agatgccttt tatatcacta tctatatctt
    17461 catctagata agaatctaaa tcttctaagt caacttctag ttcttgatat gtcatatcaa
    17521 cactgacatc ctcatctata tccatttctg atattaattc tcctaaaata ctatttccat
    17581 cagaatctaa accactatct atatcttcat ctatatagtt ttcatttaaa atttcatcta
    17641 aatcaatctc gtaataaact tcattatttt tttcatcccc tgagtccaat cctaagaact
    17701 catagttaaa agtccaaaca ttgtatttac ttgacatact ttattcctcc tgtgaaaaac
    17761 atattttcat ctagtttatt ggttccataa tcaacagagt tatatatttt ttcaattatg
    17821 tcttttcttc tttcttttgt actattattt aatgtcaatt gtttaaatac atatgtattt
    17881 gttattctct cgaataatat ttcttttata ctatcatcat caatatttct atagtttttc
    17941 cacaataact caaataactt aaacatttcc ctactagttt catctacata ataaggatac
    18001 atattttcta ctaaatggtt taatcttgat atggcttgtt ttaaaaatac atcaagactt
    18061 gcactttcac tttcttttaa cattctataa ttgtatttct tcttatcttc tcctgaaatt
    18121 acaaagatga tattgtctaa acttttttca acttcattta aataactgct tatattatat
    18181 gataaatcag acaatagatt tttaatctgc ttaaacactt gtacatcaag tttagtatta
    18241 tacttttcaa acaaatctat ttcttgattt tctaatattc ttggcatata tgctgacaat
    18301 aaatctaaat tgtttctttg ttcttgtatt tgcttataaa gtgaatttat aacttctatt
    18361 cttatttcat catttaactc tttttctaaa atcttatcaa attcagattt catatagtaa
    18421 atatttccaa agaaattaaa tattatcata tcattcatag ctttagacca attcttatat
    18481 atcacttcaa tagaattttc tttaagtaga ggttgctttg catatataaa tacatcttgc
    18541 ataagttttt gaaccatact tttacttaag tttaaaagta ctttttgatt attaaatttt
    18601 atttccgttt ctacaaaatt agaattttcg ttttcaagtt ctttattaat tacaatgtct
    18661 tttatatcac tattttttat agatatgatt tctttttgaa aagactttag aatagtcaag
    18721 ctatcgaaat cttcttgaat aaaaaatgga gcatgtttta atgtatatcc cactatttca
    18781 ttttctatat cttttaggtc atctatttgc ataatttctc tatttttatc atttatatgt
    18841 aggttaaata catttttatt atcatataat attatatctt ctcctaaatt taacttagaa
    18901 caattatctt tcgctgactt tacaatagtc ttcttagatt tcttatttat aaaaattaat
    18961 tggtcttgat attttactaa aagcatgttt gtgtttttat aatcaaatcc atctatattt
    19021 cctgatatct tgcagaatac tatttcacaa tctttaggaa gatttcctat tttttggtta
    19081 tctttaactc tctcatttaa atcagatggg actacactat ctatattgtt attaaatata
    19141 gatacagacc ttactataat cccctctctt tccatatgaa agtatccctt caatgacact
    19201 cttttatctt ctaaaatgta tctttctata tcatcaaaat ttatttcaaa tctatttttc
    19261 ccactgataa atataaactt ctcattatct ttattaattc cacccatata aaacttattg
    19321 tcatctaaac ttaatatagt catattatcc tttagataga atataaattc tctttgactt
    19381 ttgcagatta aatcatattc atccaaagtt attttcatag tcaaatttgc tgtaacattt
    19441 ttacttgaat tttcactttt tatgtaaaaa ggcaagttat ttccatagac aattatttga
    19501 gaatcttcta aaactgcctc taatttttct ttattgtctg aaaaactata tatatatttt
    19561 acacctgtat cctcattaac ttcatctgat ttgctgatta ttatattccc tagatttgtt
    19621 atatgaagct ttttatattc ttttgagtat ctacttatgc cactaagctc aaacccatct
    19681 gaaagctcat atatgatttt ttctcctaat ttcaacatac cttcaccacc taaacagaat
    19741 tataagtatt ttataaaggt gaataaaatg tctcaaaaca aataatattt tttttaatac
    19801 tttttattct gagacatagt ttctcctttt ttataatgct ctaagcacct tcaactattt
    19861 cttcataaag tttatctaaa tgtttaacat attcacgata tccagatttg ttctttaact
    19921 cacttcttaa atcttcatat ttttgtttaa gttcttcagc actcaaagaa ggagctgatt
    19981 cttttaattc ttctaactta taatcatagt acttatcata catagctata tatgtatcaa
    20041 tagacttctt agctagttca ttacattttt cttgtgcttc tccatattga gaataatcac
    20101 ctatttttat tttatagtat ccattttctt gaattattgt tgaagaaata cctttagctt
    20161 catattcttt tgctgtttct tttgctgttt tttcaacttt aagtgaacct gcataaacag
    20221 tgtaatatac acctttacca tctttatatg cttccatttc ttttgctttc ttttcagctt
    20281 gtgctttttc ttgagcctct ttttcagctt ttgccttttc ttctgcttct tttttctctt
    20341 tttcagcttg tgcttttttc tcttcttcag ttaatttatt gttagcttgt tgttgagttg
    20401 atgactttgg aatattctct ttatcatttt tcttattgtt gcctactgtt ataaaatatc
    20461 ctacaccaac aataaccact ataactactg ctataatagt ttcttttaat ctactcttct
    20521 tagaaacaac ctcttcatca tctaaagaat cttctttatc tgatacataa tttttcattt
    20581 ctttcttgtt ttctctatat ttgtccttag aagaattttt agatttttta ttactatttt
    20641 ctttcttttt aataggtatt actactgatt tttcattttc taatttttct tcttttattt
    20701 cttttgcttt tttatcttct aagtttttac tttctttttc aagcttcgat tcattatttg
    20761 aagatacttt ttcatctaag ttattattta ttggtttttc tatttttaca ctacgttctt
    20821 cttctatatg ttgacttggt tcttcttgtt ttatttcttt caattcattt tgctctattt
    20881 cttctgattt cacttgatga ttttcaactg gtttatctaa aattatatct ggagaaatat
    20941 catttaaatt ctcttcttct tgttcagctt cttcattgtc ttttttaata aatccaaata
    21001 atttagcctt ctttttactc tttttatttt ctggttcaat tatatcttct ttattgctgt
    21061 cgtactcttt atctgtagta tctttatctt ctacaacgtc acctatatct tgattattac
    21121 tatctacttc ttctttagtc ccttcttcag catcatcttc tattgaaata ctacttctaa
    21181 agcaaatatc tttatctgca ttttttatca tgtaaatgta ttcttcaata tcgatatctt
    21241 tatttcttct agttcttttt gatgcaattt catgattttc taattcacta tagttatctt
    21301 ttattagatt ctgtgcttta ttccatgtat cttcactaat ttctgtttta tttttaatta
    21361 catagtccac aaattcctta aaattttcag gaccaaattc tccaattata gtcagtctca
    21421 ttgcaccttc tataaggtct ctatttcttc tctttaaaaa ttctaataca actgttgcac
    21481 aagcaaaatc tttaataatt tctttgccaa aaccatatgc ttcaacagaa tgaagttctt
    21541 ctaatccaca taacttctta tattcattgt gtattgtttg agatatactt tcattattta
    21601 agctatttaa cttatctata aacttcgatt ctatatcatt tgattttgat tcatgtttta
    21661 cttttgaatt tttaatttca tttagtagca tgtcattttc tgaattatct ttacctaatt
    21721 gcaaatcctc aatttcagtt gcttttattt ctactttgtt ttgttcgtta ttctcttcca
    21781 cattattagg taccacatct ttattactag ctaatatttt gtttacacag aatataattt
    21841 ctctttttaa ttctcttttc gcatctgctt gatttttaag aaataaatct ccacattctt
    21901 tcttttctaa acttattttt ctaagagata tattaagaag gtctttatat aggtttaaaa
    21961 ccttataatc cttttctaaa cgtcttcttc tctctgatag ttccatttta tcataagcta
    22021 aaaaactatt taaatcattg gcttttagag agtctaatcc ctctttcata gccttattta
    22081 catactttct acattttgct gtagttaact ctaaatcttt ttctaattca tctatttcag
    22141 gtatgtactt atctactatc catttgccaa taagtggttc tgtatttctt gctaatgtct
    22201 tataaaacaa tatattaaat tttaaaagct ctgcatattt tgcttcatta aattctactt
    22261 tttcaaattt cttttccaaa acaccatcta gcttttcatt tattttagac tcttgtttta
    22321 aaaaacttgc tgtgacattt aaaacagtgt ttcttattat attattttca ctcaaaacct
    22381 tcactcctta aatcaattat tttattgtaa tatttacatt gtgcaaagtt gctaatttga
    22441 ttatgatatc taaataatta tttactaaat ttgctacatc ttatatcata tcatattatt
    22501 tacatcattt tactttttct ttttatatct atcttatatt ataccatttt taatatctgt
    22561 actaacttat attacaatat tcctcaaaat tttaaaagta gattagacaa atttacaaat
    22621 aaaaaaacat cagtttaaac tgatgttttt gactatcgaa ataattaagc aagtctataa
    22681 gcggagttct gtattagata atcatctttc taggcctatt gtcaccaata ggctcaagcg
    22741 acctacctac cggagagtac gagcagcact ctttattcct atcttggtct tgctccaggt
    22801 ggggtttaca tagccaacta gtcgcctagc tgctggtgag ctcttacctc gcctttccat
    22861 ccttaccaca atagtggcgg tttatttctg ttgcactatc cttaggatcg ctcccactag
    22921 gcgttaccta gcaccctgct ctatggagct ccgactttcc tcatgtaaga ttacatgcga
    22981 ttatctggct tacttaatat ttattttact tagttatgtt aacacaaatt cttttacatg
    23041 tcaaagtaat attatactaa aaatttaaat ttattcaaaa atagtgtaat atagatatat
    23101 aaactacatt taactcgagg tgatctattg aaaaaattat atagaatagt aattaacatt
    23161 atactagtat tagtaatact ttacagtgga tttaatatat actcaaaact taccaaatac
    23221 aatcatgata ctaaaatatc ctcagaactt caaaaaaaag aatataaaaa agaggattta
    23281 tcaaagatta acagtgattt taaattttgg ctttctgttg agaatacaaa tataaattat
    23341 ccagttgtac aatctaaaga taattcttac tatcttgaca aagattttta taaaaaagat
    23401 tctatttctg gtacactgtt tatggattat agaaataaat ctattgatga caaaaatatc
    23461 ataatatatg gacataatat gaaaaataaa actatgttca acaacctaaa taagtttaaa
    23521 gatgctgact tctttaaaaa aaataataaa ataaaaataa ccttgaatgg aaaagaattt
    23581 ttatatgatg ttttttctgc ttatattgta gaatctgatt atgattatct aaaaacaaat
    23641 tttaacaatg aatctgatta tcaaaattac ataaatgata ttacttctaa atcattgtat
    23701 aaatctccta ttaaggtaaa tagcaatgat aaaatagtta cactttctac atgtacctat
    23761 gaatttgatg atgccagaat ggtgattcat ggtagattga tttaaaactc acagacacag
    23821 tttaaaacaa taattttaaa ctgtgtcatc ttttatacct ataatttgta aattagacca
    23881 ctaaaagtat ttaatacaaa ataacaaata catcaaacaa taaaaaacaa taaaaaaaga
    23941 ctatgctaaa aacatagtct ttactggagc tggtgatagg aatcgaacct acaacctgct
    24001 gattacaagt cagctgctct accgttgagc cacaccagca cacctatttt attgtaaatt
    24061 agcaaaacta atttttaaaa gtta
//
LOCUS       DAMJYC010000057        23060 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJYC010000057 DAMJYC010000000
VERSION     DAMJYC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.058"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            43..864
                     /locus_tag="PC472_002451"
     CDS             43..864
                     /locus_tag="PC472_002451"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2727768.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIHIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(940..2328)
                     /locus_tag="PC472_002452"
     CDS             complement(940..2328)
                     /locus_tag="PC472_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2727769.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFDKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2463..3221)
                     /locus_tag="PC472_002453"
     CDS             complement(2463..3221)
                     /locus_tag="PC472_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2727770.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSAIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3223..3939)
                     /locus_tag="PC472_002454"
     CDS             complement(3223..3939)
                     /locus_tag="PC472_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2727771.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(3941..4648)
                     /locus_tag="PC472_002455"
     CDS             complement(3941..4648)
                     /locus_tag="PC472_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2727772.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(4764..5300)
                     /locus_tag="PC472_002456"
     CDS             complement(4764..5300)
                     /locus_tag="PC472_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2727773.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5402..6472)
                     /locus_tag="PC472_002457"
     CDS             complement(5402..6472)
                     /locus_tag="PC472_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2727774.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6465..7523)
                     /locus_tag="PC472_002458"
     CDS             complement(6465..7523)
                     /locus_tag="PC472_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2727775.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(7510..7842)
                     /locus_tag="PC472_002459"
     CDS             complement(7510..7842)
                     /locus_tag="PC472_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2727776.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8041..9162
                     /locus_tag="PC472_002460"
     CDS             8041..9162
                     /locus_tag="PC472_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2727777.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9290..11188)
                     /locus_tag="PC472_002461"
     CDS             complement(9290..11188)
                     /locus_tag="PC472_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2727778.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11503..12279
                     /locus_tag="PC472_002462"
     CDS             11503..12279
                     /locus_tag="PC472_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2727779.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(12371..13123)
                     /gene="xth"
                     /locus_tag="PC472_002463"
     CDS             complement(12371..13123)
                     /gene="xth"
                     /locus_tag="PC472_002463"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2727780.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(13284..14234)
                     /gene="galT"
                     /locus_tag="PC472_002464"
     CDS             complement(13284..14234)
                     /gene="galT"
                     /locus_tag="PC472_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2727781.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            14619..15815
                     /locus_tag="PC472_002465"
     CDS             14619..15815
                     /locus_tag="PC472_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2727782.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(16150..16602)
                     /locus_tag="PC472_002466"
     CDS             complement(16150..16602)
                     /locus_tag="PC472_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2727783.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(16914..18359)
                     /locus_tag="PC472_002467"
     CDS             complement(16914..18359)
                     /locus_tag="PC472_002467"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2727784.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(18503..19411)
                     /locus_tag="PC472_002468"
     CDS             complement(18503..19411)
                     /locus_tag="PC472_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2727785.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            19668..20492
                     /locus_tag="PC472_002469"
     CDS             19668..20492
                     /locus_tag="PC472_002469"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2727786.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            20532..21062
                     /gene="lepB"
                     /locus_tag="PC472_002470"
     CDS             20532..21062
                     /gene="lepB"
                     /locus_tag="PC472_002470"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2727787.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNLGKVD"
     gene            complement(21144..22925)
                     /locus_tag="PC472_002471"
     CDS             complement(21144..22925)
                     /locus_tag="PC472_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2727788.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         7750 a         3637 c         2665 g         9008 t
ORIGIN      
        1 ttggtatcat tataattaat acatactaat ggagggtttt ttatgcaaaa aaaagtcgca
       61 gcaattaatg acttatctgg aataggaaaa tgctcattat cggttgctat acctatactt
      121 tcagcattaa aggttcaatg ttgtccattt ccaacggcca tactttcaag tcaaactggt
      181 tatcctgaat ttactttctt agactttact gatgaaatgg taaaatattc aaatgtttgg
      241 aaaaatttaa aagtcaattt tgatagtata tatagtggtt ttttgggttc aaagcatcaa
      301 attgagatag tggctaattt tataaacgat tatcccaatg cttttatcgt agttgaccct
      361 gtgatgggtg ataatggagt tatgtatcca atattcacag aagaaatgag acaggaaatt
      421 aaagaattag ttaaacattc agatttaact actcctaatt taacagaagc ttgtttctta
      481 actggaaatg attataccaa aagcgattat aatagagatg agcttataca tattgcaaaa
      541 tctgtatctg atttgggacc tagcaaagtc gttattactg gtatattaga agatgataat
      601 atattaaatt tggcttacga tagagataat gaccatgtat tctttacatc agtcaaatat
      661 aataattgtt cttacagtgg aactggagat atattcacat ctatactttg tggtatgtta
      721 gtaaataaac atgatttggg agtagcggtt aatactgcaa cagattttat atataaaact
      781 ataaattaca catctcaatt tgatactgat agaaatgatg gagttatgtt tgaaaacttt
      841 ttatcagatt taaccaatat ataaaatcaa aaaaactatc cattttataa atacctataa
      901 atcataaaat ttaggtattt acaaaatgga tattatttac taaactattg gtatttcaat
      961 tgttaccata gcaccaccag ttttcacaga attttcaata cataaagtcc cattatgttt
     1021 ttgtacaata ttatttacaa tatagagtcc cattccataa tgagatttag aatttctact
     1081 caaatctcct gtataaaact gttctgtagc ttttgacaaa tctgctttag aaaaaccact
     1141 tccacaatct gtaactacaa attttaagta tttattacgt actgatacac ttatgtatag
     1201 tttactctgg cttggtgaat aatctatagc atttgaaatt atattcataa tagctctgaa
     1261 taacagttct ctatctatag ttatattttt aggcaaatta tcctgtttat aaaactccac
     1321 atctaagttt ttagtactag ctaatgcttc tatttgcact aagacatctt ttataaactc
     1381 actcatactg attgcttctt tatttaatat atagccattc tctgtttttg atatatctat
     1441 taaagtatta atatattttt ctatttgagt gacacccttt gagatatatt ccatgtattc
     1501 taataactca ggattttcat ttatttcaat taataagtca gtatttccat gtataattgt
     1561 tagtggtgtt tttatatcat gtgccagtgc agatatctgt tcctttctat tttcttctag
     1621 catccattgt ttttctaaag aatcttttaa cgccaacttc attttatcca tagaaaaaag
     1681 gacattatta atttcataaa ttttactaga ttctacaaca aaatctaaat cctgttgttc
     1741 tattttttct gtagtatttt ttaaaagctc catttcaata ttaaatttct tgtcaaatac
     1801 ttttgataat ataacaattt caataaaaaa tattatacta aaaataatca tacccaatgt
     1861 ctctggtttt ggtaaatatg ctcttaaaat tggagaacta tactctgata ctattttgta
     1921 ttttattata aaaacttcat ctttcttaaa aaactttata taactttctg aatatgcatg
     1981 cctttcttca atatctctca ttaaattcca tacttctttt gattcttttt tattaaaggt
     2041 acctgaaatt aaattcccat ctaaagtata aaccccatac tcatatatac ttggaattat
     2101 atcttcagtt actttttcac ttgaaataat ctgttcttta tatttattaa attgtttttc
     2161 tgcataatta gctggtaaaa ttacacctga tgataataat acagaaaatg ataacattaa
     2221 aaggagtaca attgaaattg tcataataaa gaataaagat aaatatttta taaaaatagt
     2281 tcttaacttt tcaacttttt tgttgttaga ttttttacct ccaagcattt gtaccctgtc
     2341 ctccaatagt ttaattaatt cataattaat acctagccag ttttttattt gattatttta
     2401 tatcttttta ttgctaagtt atattttaac ataataatat aaataaatag tcaattttta
     2461 ctctattctt ctgatttttt accttcccat ttagaaaacc aaatacataa aattacaaat
     2521 gctaaaattg ttacaaaacc acacattaaa cctgttgatt gatatgcttt tattgattca
     2581 acctttatat attcaaccgt tttagaactt gaaaaagacg accaaatagt tataaatcta
     2641 acaccccatg cacaaggaaa atactgccaa atagcagaac ccaatccagt taataataat
     2701 gctgaaagta acatttcaac tatacctact ccaattgatg ttccttttcc gaaccttaaa
     2761 cttaagaata aatgtaatat gtattcaaat aaattacacc caattaaaat tagaattgaa
     2821 atcatataaa agcttgatgt aaagtaagcc ttagatatac caaatttaaa acctaataca
     2881 gacactaatg ttgctacaaa accaagacat actaagatta tatatttact tataagagta
     2941 agatattttg gattagatgt tgttaacata tgtttataat tccctgcata atattcttgt
     3001 tcaacaaaca ttgaacacac aacacttgac aataaaggaa aagctattgc tataacttgt
     3061 aaatatgcta ctatctttag tatattatca gctcctgaaa ccttataata ccataaaaac
     3121 aatcccatcc ccattaaagc aatgcaaatg tgcattagta ttataaaatt tctctttaat
     3181 tttattaaat cagaacaaaa tattcttatt aactgtctca tattacttca cctctctatt
     3241 attgaaccaa acccttgtag ctaaagcaaa taatacaaat aaaacaatag atattaatac
     3301 tcctggcaaa ataacagcat tatttaaaaa ttcacttccc tctggtacag gaagaccatt
     3361 tggcagaata tgcaatacaa cagccattaa tcttgaagga atcgaatatg gaaatatata
     3421 ccacatttct gtagttgaga aaacagcaac tccaacaata gtgaagaaca tatttattag
     3481 aactgtaaca aaaataccaa attttgaagc taaaaacata caaagaggaa tctgccacat
     3541 aaatagtaaa ataattaata tagttgcaat gatattttga cctattgaaa tagtttcttt
     3601 atagattatc ccaacaaaaa ctacacctat tgcagaaata attgatgcta taaaacagca
     3661 aataatacag taaatcattt ttccagtcca aacatctttc atttcaattg gcaatggtaa
     3721 aataccacga tatttacttt tttcatcttt taatactatt aaagaacata ttaatgttaa
     3781 tgctcctgga agaaatgtcg tataccacca gttgaaagca gcattttgcc ctccacctag
     3841 tccacacaaa agtatacaaa acactggaac tatccatatc aacttcattc caaaagttcc
     3901 tttcatcttt tgaaattcca ctctaataaa tctttccata ttactgccct ctctttctat
     3961 tttcagctac aactttcata aaaagttctt ctaagttttg ttcttttgat acttctcctt
     4021 gataacccat tactccatta gaaattattc ctatttcatc tacaacttgt tcaacttcag
     4081 acaagatatg gcttgataaa ataactgtaa ttccttgttt tggaaaagaa cgaatcaatt
     4141 ctctaagttc ttcaatacca atagggtcaa gaccatttgt aggctcatct aagattaaaa
     4201 gttttgggtg atttaataat gcaatagcaa taccaaggcg ttgcttcatg cctagagaga
     4261 attgacctga acgcttttta aaggtatctc ttaaatctac agtctctaaa acttcttcaa
     4321 tgcgagagtc aggaagacca agtaaagtag tatggacttt taaattttct cgtgctgtta
     4381 aattctcata tatagcagga gactcaataa gagaaccaat atttgataaa tcctttctag
     4441 tccatctatg tccctcaaac attatttttc ctgaagttgg atttaaaatc ccagttatca
     4501 tttttaataa agtagatttc ccagctccat ttggtcctaa taatccatat atagaattag
     4561 atttaattgc tagtgaaatg ttatcaactg ccatctgctc cttaaatttt ttagaaactc
     4621 cttttacctg caaaatataa ttactcataa ttttcatcct ttcttttgtc aaacttattt
     4681 acaagaatca ttctaaatca taaatataag gattttataa gaattaaaaa acaaaaaaag
     4741 atgtgttaaa aacacatctt tttttatctt aattctatta attttccatt gtagtatatc
     4801 tctacttctt caatccaaat gctattatct tcttcttgga tgacattttt aataatatta
     4861 tttatagtat atattatatt ttgttcattc aaataatcat ctttccaaga aactctactt
     4921 tttttatctg gttctatcaa gtttatttta acaagattat ctttttcctt aacttctact
     4981 tccatgtcta aaccattaaa atactcattt gataatgtat ctaagattaa tcgcagttta
     5041 tccttaagtg aactttcttc atcaatctgt aagtacttta ctattttctc atcctttgaa
     5101 attatctcta taggtatttt agtagttttt ttagaattaa tcattttact agttgtttct
     5161 tcttttgcat cttcatcctt ctttacaact gctgattctt tagaatcaac tagctcaact
     5221 gcactgatgc tataatccat acatgccgta ctcaataaag tagtagcaat tattaaaaaa
     5281 cttactgcta ttttattcat aagtatccct ctcctaattc tctttcgata cttattatac
     5341 catagtatag attatatttt tatactttta aatcaattta tgaattatta tagtaaatta
     5401 tttacaattc taagctattt gaatttattt ttatagtata ttctttctct aaattcgaca
     5461 gatttttatt caaaagccct tttatttccc tcgtatttct atacttgttg ctatgttctt
     5521 cattatgttc ataatcatat gaatcattgt catattcttc atcattatta aattcttcaa
     5581 ttgcttcttt tgactttata tcacaattaa atttatactt atttactggt atatcaagtc
     5641 ttaccttaga atttggaact tctatgtcca ggaacttagc aattggcata tcagagtaca
     5701 agtaaaattc actttcataa tttgaatttc tacctatttt ctcttttata tttacatttt
     5761 caggtatata ctcttctata ttattgataa atacatctcc attctctgaa gatatatcca
     5821 atgactctga ttctatgttt acatttttaa cgcctaaaat tgactttgtt gatagatgaa
     5881 gattgctatt agatattaag tttaagtttt ctaatctatt tactttattt tctgttatag
     5941 ctctgctaaa acctccagaa gaagttttat atgttatatt atttgaatct acatcatcag
     6001 atataaataa gtacgaactt atacttgatg catttatatc tacattattt ggtacatata
     6061 ttctaatttc tctataatca cgtgaaaaca tactatttat agatgtattt aataagtggt
     6121 cgaaacttct aatcttttta attttatttt cagattttct ttcgcctttt acaactaaag
     6181 tcttatcttg tagggttaca ctgtatttgt aaaattcatt attccctctt gttgtcacac
     6241 gcacatcttc atttgaactt tttcttatca taacatttat attgtcaaca gctatatcta
     6301 acttgtcaat atttactttt tcttgataaa gtttgttttc tttgtttaat tcagcttcct
     6361 tttctaaacc aaagtttatt aggcttggca taactaaaat ccctgaccat gttgtcccta
     6421 ttatacctat taccaataaa accgcagcaa atatagcaag ttttttattc atgtttatct
     6481 tctcctttct tattttctat atcttcaagg tccttatata tcgcctcaaa atttatacta
     6541 tcatcattat attcatcagt ttcttttatg ctttcttctt ctctattata gtaatcatta
     6601 ttatatgaat cttcattaat gtctttattc aaatcttcat catttaaagt agcttttttt
     6661 cttttttcca tcttttcatt tgcctttata taaatatttt ttctctttat ccaatgtata
     6721 taattattta gtaattgttt acctagtttt atggcaaata ataaaatttg ccatgcaagt
     6781 atctcaagcc ctacaaatgc tatggttgcg aaaatacaaa acatcataac atctggaacc
     6841 attgctataa atttaatcac aaatggaatt aatataacaa aagcaattaa tgagcatact
     6901 aatgcaatag tagcagatac aatagcacat acaaacgcaa atgctggaac aattagaaat
     6961 aaagataaca ctactaataa cccctttata acttgtctgg ataatcctcc tacaccttct
     7021 tctatacttg gtgtaagttt atcatttata ttttcttttg atttattgta cttttcctta
     7081 gcttcatctt tcaagataga gtaatccttt tttacatttg atttaaatct actaaattta
     7141 ccatttaata tgtctgtatt cattattttt ccatctatat tttcttttga tttactatac
     7201 ttttcttttg cttcatcttt taagatagag taaccttttt tcatatctga tttaaattta
     7261 ctaaacttat cattcaaaac atctgtattt attatttttt cctcttcaat atttttttct
     7321 ttagtttgta agataagttc ttttgctata gattttggtg agccaagtga tgctattatc
     7381 tccatatcac tctttccctc tataagacca tctacgaaat actcttcata atctctcaat
     7441 atatcattaa cttcgctctc agaaaaatct ttttttagat aatcctttaa aatttcaaga
     7501 aactcagttt tattcaagtt cattacctcc ctcatcttca cttattaaaa tctcaacagc
     7561 ctttacaaat tctctccact cttccatcag attggataaa ttctcttttc ctagtgaagt
     7621 tattctatag tattttctag caggaccttc attagattct agtatatatg tttcgaaata
     7681 caaatctttt gtaagtcttc ttagaatagg atatattgtt ccttcattta catctattac
     7741 tttagagatg ttatgtacta tctcatatcc atacatatct ttcttagata tgagagcaag
     7801 tacacaaatt tctaaaacac cctttctaaa ttgtgtgttc atatactcca tcctttcttt
     7861 aaatacacag tattgtgttt tacaaggtac tatgttaaaa cttcttatat cctcataata
     7921 acacagtact atgttctgca caatagttat ttttaaatat tcttgttgat taaatccttc
     7981 tataaatgta taatactcta atcagatata ttttatgaaa gtggtgataa attggctaaa
     8041 atgaaagatg ataaaacaat attaatccta actgctcaat ttggtgctgg acatataagt
     8101 gctgcaaaag cagtcaaaga atgtattatt gaaaaatata gtaactataa tgttgttata
     8161 caaaacttta taaatgcaag tattcccatg atgaataagc ctatggtaaa actttatgaa
     8221 aacaatacaa aatatactcc tggattatat aattactatt attactttaa gaaatcgttt
     8281 gattcaagac atgatttatc tcataaatta tatacaccta aactttctga gtatattgct
     8341 gatataaatc cagatttaat tatctctaca tttccactag ctgctgcttg tgtaaataac
     8401 tttaagataa aaaatcctga tataaatata cctacactta cagttataac agatgttgta
     8461 gatagtatgg aatgggtttt tgaaaataca gatttgtatt ttgttccttc tcctgaaata
     8521 aaaaatagat tttttcagaa aggaataaat cctgactcca taaaggttac tggagttcct
     8581 gttgataaga gatttcaaat tgaaagtaaa gaactatgtt gtgataaata tagactatta
     8641 cttctaggtg gaggaagagg tctatttgat atagatgagg attttatgca ttggatagat
     8701 gaatttattg aagaacatag taattctata gaaattacaa tagttactgg taaaaacaaa
     8761 aaattatatg acaatctaac tcataaaaaa cctctaaaaa atattaaagt acttggattt
     8821 gtaaacgaca tgtataatct aataagagaa tgtgacctaa tgctaactaa gcctggagga
     8881 gcaactatat ttgaagctat tcaatcacaa actccagtac tcgtaaaaat gccaaaagtt
     8941 ggacaagaaa ttgaaaacgc taaatttata atagataagg gtcttggcat gatttatagt
     9001 gatgacctag accttaaaaa catattttat aaattagtat caaatgaatt tgattctata
     9061 ataaacttta tgaaaaaaaa tttaaaagaa tttaaaacag ttattcatcc agagaaaatt
     9121 gctgattata tatcagaatt aatagataat cattacagtt aaattgtact attaaaaaat
     9181 ttcaaacata aaattaaagg caatcaagta tgctcttaca cttgagaatt tactcatagt
     9241 gtttaataat acttgattgc ctttatcata tcaaaatcaa attgttatat tagtatatat
     9301 ttttcttgta ctgcatatac agttgttgta tttcttccat acttctcttt aattctattt
     9361 gtaaatctga agcagtctgg taatccttat tttctatagc ttctctcact ctagtgaata
     9421 gtgattctct agttaaagta tccttcataa tattgaactt taaatccgat attgagttcc
     9481 aaactacctc atcaagcgca tccatatttt cttttgtgtg tatcttgaca atacttctta
     9541 ttttttgaat cccagcctct attattctag tttttaattt cttttgccac ttatctatag
     9601 ctccaatctt aaatgattct attatactat cagtaaatac atcaccttgt tttaaagatt
     9661 ttaattttga agcatatatt tctaagtttt tcatattttc ataaactgta gctggtggta
     9721 ttccaaatct tgcatttctt tcttccaaac tataatgttc aaatacatca ttttcatctc
     9781 tatatgctct atctttttct aaatagaaac tttcttcacc tacattttta gataactctt
     9841 tttcaagttc ttttgtacta agaccactct tagctgctgc ttttatacca tccaacattg
     9901 tttgataaca tcctgctatt actaaatacg tgttagatag agggcttggt gatcttaact
     9961 caaatcttac agtctttgga tttttcatat ctcttattaa tccaacaagc actgacctat
    10021 ttcttgatgg aatctcataa ctatgcccta aagatgtaac tatgcataca ggagcttcaa
    10081 atccaggaac taatctatta aagccatcat tagaagctgt tacaattggg tttaatactt
    10141 cataattgta aagaagtccc ataagagccc catatcctag ttcacttaga taatcttctt
    10201 ttaaatcttt tggagcaaat agatttttta tacttccatc ctttaacttc gcacttactc
    10261 ctacatgtgt atgtccacca cttcctgcta ctccatgaat tggttttgct ttaaatgtaa
    10321 cttctagtcc atgtgatgta aatacatcct ctataacatc tcttactaat aattcattgt
    10381 ctgctgcttg taatggtgta gaaaacttcc aagaaacctc taattgctcc atagcatggt
    10441 ttgttcttcc atctatacta atagaacttt gtattcctcc gacctctttg tgagccattt
    10501 cagggtttac acctagcttt tgaagtataa ttaaactttt ctctaaacaa gttcttataa
    10561 taccatgagt tcttttccag tattgctctt ttaggctttg tgatacatat aatttctcta
    10621 agtcagcttt atcctcagga gtattaaccc agaactcaag ttctgtagct gctgttaaca
    10681 ttatttcctc tatatcatct actgaatcta ttcctatgtt atttattaca tgtggatatt
    10741 ctctaaatat ttcatacatt gactttttaa agtacttatc tgctttttga agtactcctc
    10801 tagagcatac ttttttatta tcatgtatta aaaatgcagg tatctttaat gttccaacag
    10861 gtagacctac ttcttcgtct atatattcca tattgtaatc tacataccaa tgacaacttt
    10921 tatctggcat taaatctact ttcgcattat ttaatgtagc tatattataa agctcaacac
    10981 ttgaaccatc agtctgtata gctgattcca aaaatttatc tatatcctcc aaaaataatt
    11041 ctacaggaat cttttcatct gttgcatttc ctcctaaatc aacccccatt aaggaaacaa
    11101 atcttatatt tttattgctt tttaggattt ctcttaattc ttcattagtg tgtttatctt
    11161 tctctattac gtataataga ctatccattt ttccactctc ccataatttt tatgtatatt
    11221 ttattttgtt aaatacaaga taaatttcat tttccttaca tttatgaatt tttcttgcaa
    11281 attattcgtg ttttattcgt gtttttattt atattattat atatcaagta taaaaagttc
    11341 gcaatatttt ttattggaaa aaatattata catacaatag tttaacattt ttacaaatat
    11401 ggggtatact aaaataaaag tatttttatt tgaaaattta attttattta taatataatc
    11461 tatagtaaaa gaaataataa aaaaatttag gagataaact ttatggaata cataattttc
    11521 gatttggaat ttaaccaagg atttgataaa aaactcaata aaactgtatc caatgaaaag
    11581 tgtccttttg aaattataca aataggagca attaaattgg attctaaatt caatataata
    11641 gatacgttta atagctatgt aaaacctaca atttataaag acatacatcc ctttattagt
    11701 cgtatgacta atattaaaaa ttctgatttt aatgattcgc ctactttccc tgaggtctac
    11761 aacaatttta taaaatttat atcatcacaa gaccctatat tatgtgtttg gggagctgga
    11821 gatttaaagg aattatacag gaatatcaac tatcacaagc taccatcaaa ttccttacct
    11881 aaatcttata ttaatataca acaacatgca tctaaatatt tcaataatcc agcaggaaaa
    11941 agcattggcc tacaaaatgc tatttctata ttagaacttg atgaaaaaat gtcttatcac
    12001 aatgctttaa atgatgcata ttatactgct aaagttttta ttaagatata caatccaagt
    12061 atagtcccag atatatatct ttatacgagt ataaaaccta aaactataag atattctaac
    12121 aaaaaaagag ttgattatga taagttattt gatgaattta ggaaaatttt aaatcgtgaa
    12181 ttaactaaag acgagaaaaa aatcattaac ttagcttata atatggggaa aacaaatcaa
    12241 tttacattag aaaatgttaa gcaaagaaaa aacaaataga gtttatttag ataaaaaata
    12301 ggcatgtaga ttaaaaaact agttaacttt aaatttatat ctacagccca atttttatca
    12361 taattaattt ttattttatc tctaattcaa ctggacaatg gtctgaacct aatatttcag
    12421 tgtgtatatc cgcacttact aatctatctt ctaatttttt ggatgcacaa aaataatcta
    12481 ttctccaccc tgcattattt tttcttgcat taaatctata tgaccaccaa ctataaacac
    12541 cttctttatc aggattaaaa tatctatatg tgtctataaa cccactgtct aataatttag
    12601 aaaacttctc tctttcttct ttagtaaaac ctgcattttt catattattt ttaggatttt
    12661 ttaaatctat ttctttatgt gcaacgttca tatctccaca cacaattaca ggcttatgat
    12721 tatctagttt ggttaaataa tctataaagt cgtcttccca tctagttcta tattctagtc
    12781 tctttaattc agattgagaa tttggcgtat atactgtaac aaagtaaaaa tcttcaaact
    12841 ctagagttat cactctaccc tctttgtcat gttcttctat atttattcca tacattactt
    12901 ttaaaggctc tttttttgta aatatagcag tacctgaata accctttctc tcagcataat
    12961 tccaatattg aaaatatcct ggtaaatcta actctatttg cccttcttgc aatttagttt
    13021 cttgtaaaca aaatatatct gcatctactt ctttgaaaaa gtctaaaaat cctttcccaa
    13081 cacaagctct tattccatta acattccatg atataaattt catacttata ctcctcatct
    13141 aaacattgac acactcccca tagctaaaga tgggggcttt tcaacaattt tattgtaaat
    13201 tcttaattca cttttatttt aacaatgcta tactataatt tcatctaaaa ttactattta
    13261 cttaatacct aaaaatatat gttttaatca atttttaact tttttgttaa atcttcaggg
    13321 ctcattgaag aaacatacat atttgttcca agttcaaatc ctccaaaatt atattttctt
    13381 gggattatgt gcatatgaac attaaaatac gattttgttt caatctttgt tggatgagtg
    13441 tgaatacata agttaaatgg catatatcca ttgatattgt ataacttctt aaacaacttt
    13501 tcaaatattg tagaaagctc ctgtatatta ttatcattta actcaccaaa ctctatttta
    13561 tctttaaata ttattcttac ttctccacta tacttggttg catatggtat aagtaccaaa
    13621 aaagcttctc cattataaac aactcttcta tctaaactta tttcattttc tattaaatca
    13681 tcatacaaag agcttttatt gctgtcataa tactcctttg aaaccaacag ttcattcgta
    13741 atttcaggtg gaataaaaga catagacaat atttgagcat gtggatgcat caaagatgct
    13801 cctgcctttc tcaagaagtt tttgaagata ctaacatact ctactttatc atctttcgac
    13861 aaatctctaa atctatttct atacataaaa aaaacatctt caaattcttt ttgactcatg
    13921 ttataaaaac tacgattatg tttatcgctc tctatcatta cttcatgaac accatataca
    13981 tctcttgcca tatcatcaat tataggaaat ttattgtaga cagattttac aagccatcct
    14041 tcttcactat ctatttcaaa taatgcatca gttgcatgag cttcatttcc tttgcaaaat
    14101 gggcattctt catcatattc attagtttct tcatcttcat ttgggatatc tgctgtatct
    14161 aaaggtcttt ttaacctatc tgtagcaaat ataacaacat cattggtaat tggatctatt
    14221 cttaattctt tcataattaa cacctcttag ttaatagcct ttaatataat tatatgatat
    14281 ttatcgtatc tgttaaataa atttataata ttttttataa ttttgcatat ttattattat
    14341 atattttgtc tatagctgaa agattataac taaaaaatca ctatcagtac atcagaatat
    14401 tatcttacta taaattaatt tatgaataca taatttagtg gcataaacat gggtatttta
    14461 ctataatttg ttaaaagttt attttaaagc tatattaatt ttttttgatg atttttaccc
    14521 ataagtatgt tattattatc ttgtaaagat gtcttgtatt caaacataca atttacataa
    14581 tacaatatac gtatttaaat taaatagggg gtattaaaat gaattattca aacagagtta
    14641 gtgcaatgca agcctctcca atcagaaaac ttgttccatt tgcacaagca gcaaaagata
    14701 aaggaatcaa agtatatcac ttaaacatag gacaaccaga cataaaaact cctaaaggat
    14761 tttttgatgc tgttaaaaac tttgatagcg aagtcttaga atatgctaca tcagaaggta
    14821 ttccagaatt attagaagca cttcaaaatt actataaaac ttacgatatg aattttgaaa
    14881 aagatgaatt attagtaact aatggtggta gtgaagcttt acttttcact atgatggcag
    14941 tttgtgaccc aggagataat ctattagttc cagaaccatt ctatacaaac tacaatggat
    15001 ttggtcaatc tgtaaacgta gaagttaatg ctgttacaac aaaggcagaa aacggtttcc
    15061 accttccatc taaagaagaa atattgtcta aagtagatga taaaactaag gctataattc
    15121 tttctaaccc aggaaatcca actggtgcaa tttatacaaa agaagaactt aatatcctag
    15181 ctgaaatagc taaagaaaaa gatttatgga taatagcaga tgaagtttat agagaatttg
    15241 tttatgatgg gcttgagtat acaagctgtg gtaacttaga gggtgtacag gatagagtta
    15301 taataataga cagtgtttct aagagataca gtgcttgtgg tgctagaatt ggttctatcg
    15361 cttgcaaaaa taaaggtctt atcgctcaaa tacttaaatt atgtcaagga agactttgtg
    15421 ttcctacatt agagcaaata ggagctgttg agttatacaa aactcctgtt tcttacttca
    15481 aagaagttaa tgaagagtac aaaaaaagaa gagatgtatt atacaatgaa ttaatgaagg
    15541 ttgaaggtgt tatatgtaaa aaaccaactg gcgctttcta tatagttgct aaattaccag
    15601 ttgaaaatgc agaagatttc acaatttgga tgttaaaaga atttaacaaa gataatgaaa
    15661 cagttatggt ttgccctgct gaaggattct atgctactcc aggtttagga cgtgatgaaa
    15721 taagattagc ttacatctta aatgaaaaag accttcatag agctgctaca ttattaaaag
    15781 aaggtttaga gcaatatgta gctttacaaa aataattttt taaacttaat ttatataaat
    15841 ttaatgatat aaaatgtccc cttttcagat attatttgaa aaggggacat ttcttaatta
    15901 atatattaat taagacttag acctgactat ttttcatttt ctttatatag tgttggccag
    15961 taatctttat tcactttaat taaagcatct aaaacttgtc ttgccttagt tagagaaatt
    16021 ataaaaaagt acatcaagaa aatttaagat gtacttttta gattgtattt agagttataa
    16081 ttatatagtt aaatgaataa caaagaatta atcttagtac cattaaatac aaaaattttt
    16141 atattttttc tatctaaatt cttctttatt taatatatat tgaagagtat ttattgcttc
    16201 taaatatttt tttctgtctt cttcagttaa tttagaaagt ttttctccta aaccatcact
    16261 taatttatcc ataaaatcag ttgctatctt atctccctct tcagtaatgt caattagata
    16321 ttttcttcta tcaactaaat ctcttttcct tgttacatac ttcttatcag ttaaatcatc
    16381 aaccatacta gttaaactgc ctttttctat atttaactta gagcataaat cagtcatact
    16441 tattactcca taatttttta tgaaaactaa tgctcttaat tgtgttttat ttacattata
    16501 atctggtgca aattttttta ggtcttctaa gtacaggcta gaataaatct taggaaatgt
    16561 ttttactaaa aaacttatgg tatcttttac tatatagttc atataatcac ctcggttgtt
    16621 ttaaaattta ttaaattatc taaataactt tatttcagtt tacattaaaa atattgcaag
    16681 tacaatagtt tttgatgaat gtttaaattt aattagtaat cttatcattt ttttattttt
    16741 atttatatta ttcctaatta aatattattt atatagctat tttattaaat tcatggtata
    16801 gtaagtaaag aagaactctt cacagcggtg gagagaagac gctagcccat taaaatggct
    16861 atcttattta taaataagat agccgttttt aattatattt tatctgtaca attctatact
    16921 cttaggtcta catctacttt tcctaagtct tccttataat cttttatagc aggttctaca
    16981 acttcattta caaattctac aacttgttct ggagctctac ctataaagtt ttttgggtcc
    17041 attatagata gtatttcttc cttgctcatt ttaaatgaat catcattcat tataagttca
    17101 attaagttat tatccttgcc ttcatgcttt actctatagg cagctttcat agaatattct
    17161 cttattattt catgtaattc ctgtctatct ccacctcttt ttacagcttc cataagtatt
    17221 gtttctgtag ccataaaagg aagttcttca tttacacgtc tatttatcat attttcatat
    17281 actactaaac catcagttac atttatccct atttcaagta tagcatctgc tgccataaat
    17341 gcttgaggta ttgccaatct cttattggct gagtcatcta atgttctctc taaccattga
    17401 gtggcttgta ccattgcagg gcttatagat tcagagatta tatacttaga taaagatgct
    17461 attctctcac ttctcattgg attcctctta taagccattg ctgatgaacc tatttgattt
    17521 ttttcaaatg gctcttctaa ttcttttaaa ctttgtaaaa gtcttatatc attagtcatc
    17581 ttatgcatac tttgagctat ccctgatagt atgcttatta cttgataatc taactttcta
    17641 gtatatgtct gtccacttac tgcataggaa gaatggtatc ccatcttttt acaaattttc
    17701 ttatctaact cttttacttt ttcgtggtct ccttcaaata agcttaaaaa acttgcttga
    17761 gtaccagtag tacctttaac tcctcttaac ttcatgttgt ctattctata gtttaaatct
    17821 tccaaatcaa gaattaaatc ttgagcccat aaacaagctc tttttcctac tgttgtaagt
    17881 tgagcagctt ggaagtgtgt aaatccaaga gctggcatat ctttgtactt aagtgcaaat
    17941 tcttttaaat tgtttattaa atttacaagc tttactctta gaagttttaa agcttcattc
    18001 atttgtataa catctgtatt atctccaaca tatgcagatg ttgctcctaa atgtattatt
    18061 cctttggctt tttcacattg taaaccataa gcttttacgt gagacattac atcatgtctt
    18121 acttcttttt ctatttttct agcttcatca aaatttatat tatctatatt agctcttagc
    18181 tcatctaatt gttcttcagt tatattgatt cctaactcat gttcaccttc tgcaagtgca
    18241 acccataacc ttctccatgt agagaattta aattgtggag aaaatatata actcatgtcc
    18301 ttgctacaat atctgtctgt aagtgggttt gagtaagtag tatatttatt atctatcatt
    18361 atattagcac cttccttaaa tcttttgtcg tgtgattact gtgtacacat tagtacataa
    18421 ataattatat ataattttac gaactataac aagacttttt tattttttgt tcacatttat
    18481 atatagtatt ttttatttat tactattttt tatttatatc caaattatct ttttcaatta
    18541 agtaatccac tatttcacta tcaacaaaat ttaatacata cttatatcta attgcatagt
    18601 atttatcact ccatccaagc tgatttttta catcactttc atctgcccca agccttaaac
    18661 ttattgctgc aaatgaatgt ctaaaatctc ttgctgaata ttgagaaagt ccagctaaat
    18721 ctaatgcttt tttgactata agtctgacat ttctatctgt tatagaatta ctcttttgag
    18781 ttgtaaaaat aaaatcatca ctaggtatta ttacctctgg aagcccagaa taattcctgt
    18841 aatcttctaa aagtttaaaa aagtatggat gtaatttaac tactctttct tttcttccct
    18901 tacctaacct tacatatgag ttatcgtttt catctaccat aaggtctttc cactttaagc
    18961 taactaattc atttaataaa cacccagtag taactagaca gactattatt actctatctc
    19021 ttatatttaa ttttggtagg atacctataa gtttatttat ttcttgtata cttagaacat
    19081 catcttttgt ttttattctt gttactgtgg gtttttctac atatctaaat ggatttatat
    19141 ctatgtatcc ttcttttttt aagaagttat aaaaactatg taaataacta tatatctttt
    19201 cacaagttga tttcgcatat ttagtctgta tatagtctac aaaattttta caatcttctt
    19261 tcgtagcata aatcaattct ttccctttta aaaattcttt aaacagtata acctttgata
    19321 aataatcgtg tttcgtgttt ggatttaatt ttgtagaaaa aacattaaaa gtttcatact
    19381 cataatcatc tagtattttt gtactataca taataactcc tttccttttc ataaaataaa
    19441 atagctatac ttttactata ccatatattt ccccctcaaa tatacccctt attcaataga
    19501 taacttttaa gtttattttg ttatattatg aaggttttat gaaaatatca atcttccatt
    19561 gtgcactaca cagtactatg gtattataag ttagtaaaag gtggcatttt tttattaatc
    19621 tatgaataaa atatgctact ataaatttgt attggaggta ttttgatttg gaaacattta
    19681 gtttgcttga aatttttaag gcagtcattt tgggcatagt tcagggaata acagaatggc
    19741 taccagtaag tagtacaggg catatgatat tagttgatga gtttataaaa ttgaattttt
    19801 caaatacatt tataagtaca tttttagttg taattcaatt tggttcaata ttagctgtac
    19861 ttgttatatt ttttagaaaa ttaaatcctt ttgatagcgc aaagaatatt aagcagaaaa
    19921 aagaaacagt ccgtttatgg cttaaggtta taattgcagt tataccatct ggagttattg
    19981 gaatactgtt tgaagatgat atagatagat tattctttaa ttcaacagta gttgctattg
    20041 ctcttattgt atatggtata ataatgatag ggcttgaaaa gagaaataaa aaacctaagt
    20101 ataaagattt ttctcaagtt acatataaat tagctctttg tataggatta ttccagtgtc
    20161 tagctcttat accaggtaca tctaggtctg gttctactat aataggtgca gtattacttg
    20221 gtacttctag atatgtagca gcagagtttt cattcttctt agccatccct acaatgcttg
    20281 gagctagtgc attaaaactt ctaaaagctg gatttggatt tactggattt gaatggctta
    20341 ttttaggtgt tggttctgta gtagcatttg ttgtttcaat tgtagttatt aaatttttta
    20401 tggactatat caaaaaacat gacttcaaag tatttggtta ttatagaata gtcttgggta
    20461 tagtggttct tgcttatttc tttctattat aatgtattct aatttaataa caaatgataa
    20521 ggagaaaagt tatgagtgtt aaaaaagaaa tatttgattg gattaagtca atagctatgg
    20581 ctattgtact tgcatttgta attctacaat ttataagacc ttctattgta agtggagaat
    20641 caatgtatcc tactttagat gataaagatt atctgatttt aaataggata tcatacaagg
    20701 ttggtaaacc tgaaaaaggc gatattgtag tttttaaaac caatttagtt gatggagaaa
    20761 caggaaagaa aaaagactta ataaaaagag ttatagctac tgaaggtgac agaataaaaa
    20821 tatcaaattc taaagtgtat gtaaatggaa aattattaaa tgaaccatat atacacaata
    20881 actatacttc tggagatata gatactgttg ttccaaaagg taaactattt gcaatgggag
    20941 ataatagaga aaatagtaat gatagtagat tccctgatgt aggtatggtt gatgaaaatg
    21001 aagttcttgg taaggttatg gtgagactat tacctcttga taatcttggg aaagtagact
    21061 aaaaaataag agactgaatt ggtaaagttt attttacaaa tctcagtctc tttttatttt
    21121 cttaaattac aaatattttt ttactaaata tcttttgatt ctgtaataag tcttattata
    21181 tcacttgctt cttcattaaa agttgttact atacttaggt agtctgttgc tctactcata
    21241 gctaaataaa ccttatttaa atctcctaca aagtcattta cttgataatc ttgttcaata
    21301 tcatttatag tttgattgta taacatctct aattcacata ctattaccgc tttatactct
    21361 aaattcttta ttgcatatat atttgctatt gtagttccta ccttcttact tatatttgtc
    21421 aaattatctt ctgcacatat ataaggaatt tttgcctctt ccaatgcttt ttttaacata
    21481 tattgaaagt aaatggtttt tccacttttt aatttcttct tattgtaagg gtaaacgata
    21541 gcaacttcag aataatcaag acctttttta cttattaaat actcaatttc ccagattaca
    21601 gaacttattt ggtcatcaag gtcacttact tttattatat caacagactt atttccacct
    21661 ctaagtgctt tagtataaaa gaaagtacta aattctgtat ttggtcttaa ttcgttgata
    21721 tatgagttag aattatttga aaatttattt gtaaaatcta caatctcttt agcttgtcta
    21781 taatttttac ttaagataat cttatcatca aattctatac cattatataa ttttttaaat
    21841 atattcaatc tattagagat attcaaggag ttacaagaac acacattaaa tatgaatttt
    21901 gttttatata aaaactccct tataaaatct atttcatact ctaaaaagct ttctgcttca
    21961 tcaataaaaa tagccttgaa catattttta ttttttataa tgttctgtgc ttgttttaca
    22021 agattattaa aggtcttttc gtaatcattt tttaacatgt tataatctac aattaaattg
    22081 aattttttag ctagtttaaa tataaaagaa ctaaatgtgt gaacttctaa attgttatta
    22141 tctttgtata ataactctat cctctctcga agctcattgc acaattgttt tgtatgtgta
    22201 aatatgacta atttatgatg tggatatact tttgctaatt ttatagccct agaaagcata
    22261 attgctgttt tacctatacc agaaccacct tcaataagtg tattcccata tttaatagaa
    22321 acaacatctt taatttgaac gtcttccatc atagttgctg tatacttata gtcatcattg
    22381 tagaatgaaa tttttttaaa tttttcattt agatgaagtt tatcatttat tatatagtat
    22441 tctgaacata tgtctagtaa aaataaattt aagtttattt catcattctc atcattaaga
    22501 tattcctcca gcgaaccctt attcattata tcttgaagct tatttttatc tattatatta
    22561 ttattaacaa attcttcaaa ttcatatgtt tcctcaactt caacgtatgg cataatgtaa
    22621 acgtagttat aacttatatt tctattcttt tgtcccattt ttagtttcaa aagttcatat
    22681 tcctcattca taacttcaag taactcttct tctaaaataa aaaatatatc ttctgttgta
    22741 tccataaatt ttataaataa aattcttcca tctttaacat acaacaaatc ggtatttatt
    22801 cctttaaaag gagtaacttt tggcactact ttaacgtcct cattgatatt ttcgtaaaat
    22861 attttctcga actccttcaa ttttagggcg tctaactgag aatttatatt tatgtattgg
    22921 ctcacttaca ttacctcctt ctgttatact taattatacc attttgaact atatttttat
    22981 atatactttt tataggcaaa tttagtaaat aaattcaaaa taaatacaaa taaatacaaa
    23041 actgctaaaa taatgttgca
//
LOCUS       DAMJYC010000058        22530 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJYC010000058 DAMJYC010000000
VERSION     DAMJYC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.059"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            345..1136
                     /locus_tag="PC472_002472"
     CDS             345..1136
                     /locus_tag="PC472_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2727789.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALTAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1165..2385
                     /locus_tag="PC472_002473"
     CDS             1165..2385
                     /locus_tag="PC472_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2727790.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2405..3100
                     /gene="pxpB"
                     /locus_tag="PC472_002474"
     CDS             2405..3100
                     /gene="pxpB"
                     /locus_tag="PC472_002474"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2727791.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3130..4116
                     /locus_tag="PC472_002475"
     CDS             3130..4116
                     /locus_tag="PC472_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2727792.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4335..4901)
                     /locus_tag="PC472_002476"
     CDS             complement(4335..4901)
                     /locus_tag="PC472_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2727793.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5253..6320
                     /locus_tag="PC472_002477"
     CDS             5253..6320
                     /locus_tag="PC472_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2727794.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6339..7499
                     /locus_tag="PC472_002478"
     CDS             6339..7499
                     /locus_tag="PC472_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2727795.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAINMSLGL"
     gene            7512..9608
                     /locus_tag="PC472_002479"
     CDS             7512..9608
                     /locus_tag="PC472_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2727796.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTVITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9767..10111)
                     /locus_tag="PC472_002480"
     CDS             complement(9767..10111)
                     /locus_tag="PC472_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2727797.1"
                     /translation="MEIKRYEGTGRMSRTVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10403..11293
                     /locus_tag="PC472_002481"
     CDS             10403..11293
                     /locus_tag="PC472_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2727798.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11511..12797
                     /locus_tag="PC472_002482"
     CDS             11511..12797
                     /locus_tag="PC472_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2727799.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12886..13329)
                     /locus_tag="PC472_002483"
     CDS             complement(12886..13329)
                     /locus_tag="PC472_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2727800.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13353..14147)
                     /locus_tag="PC472_002484"
     CDS             complement(13353..14147)
                     /locus_tag="PC472_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2727801.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14713..15189)
                     /locus_tag="PC472_002485"
     CDS             complement(14713..15189)
                     /locus_tag="PC472_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2727802.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDDPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15290..16459)
                     /locus_tag="PC472_002486"
     CDS             complement(15290..16459)
                     /locus_tag="PC472_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2727803.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16622..17485
                     /locus_tag="PC472_002487"
     CDS             16622..17485
                     /locus_tag="PC472_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2727804.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDTKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17556..18149
                     /locus_tag="PC472_002488"
     CDS             17556..18149
                     /locus_tag="PC472_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2727805.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18400..19035)
                     /locus_tag="PC472_002489"
     CDS             complement(18400..19035)
                     /locus_tag="PC472_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2727806.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(19115..19846)
                     /locus_tag="PC472_002490"
     CDS             complement(19115..19846)
                     /locus_tag="PC472_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2727807.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            20177..22366
                     /locus_tag="PC472_002491"
     CDS             20177..22366
                     /locus_tag="PC472_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2727808.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         8648 a         2578 c         3774 g         7530 t
ORIGIN      
        1 atgaatgaag aaaaatgatt ctgtcttgca tataaagtga ttaaaaatga aaaatacttt
       61 aaaaatgaag gttaaagccc ttattttaag tgaaaataat taattattaa ctaaaaaatg
      121 gtaaaaagat tttttgaaaa agaaatacaa atgaatgttc aattaaaata attgcaaaaa
      181 tattccttaa atggtaaaat tatattaatt aaatgcaata gaatagttgc aaaaataaaa
      241 ttgaatacaa gtgataaaat atttcatgca atatataaaa taaacaaaat ttaaaaatta
      301 aaactactta ttctatcaaa atgttagcga ggaggggtca taaattgtat aaagtagatt
      361 taaatagtga tttaggagaa agttttggaa cgtataagat tggtttagat gaagaagttt
      421 taaaatatat ttcatcagct aatatagctt gtgggtttca tgcaggtgac cctagtcata
      481 tggaaaaaac agtacagcta gctaaaaaaa atggagtgaa aataggcgca catccaggtt
      541 tcctagattt aataggattt ggaagacgtg agatgaaaat aacaaagcag gaagctaaag
      601 attatacaaa atatcaattg ggtgctttaa cggcatttgc aagttcaaat ggatgtaata
      661 tacaacatgt aaaaccacat ggagcattat acaatatggc agctaaagat aaagagttag
      721 ctatggggat ttgtgaagct atatatgaag tcgataaaga tattatactg ttggggctat
      781 ataatagtga aatgataaat tctgctaaag aaattggact taggtttgca aatgaagtat
      841 ttgcagatag agcttatgat aataatggtt ttttggttcc aagaaatgta gaaggagcag
      901 ttatacatga tacaaaacat gcaatagata gggttgttag gatggtaaaa gaaggaactg
      961 tagaaacttt aacaggtgaa gttatacata tcaaagcaga ttctatatgt gtacatgggg
     1021 acaatccaaa agcaatagag tttgtcaaag aaataagaaa gcgttttgaa ttagaaggta
     1081 tagaagtttg tccattagaa aacatagaag tttgttcgtc agagaacata gtataagact
     1141 gataacatga gggggagata aaatatgagt acaaaaaata ttacagataa aactaaaaat
     1201 aaaaaagatg tcggagcact tataggggct gcatttataa tggcaacatc agcaattgga
     1261 ccagggtttt taactcagac tgcacaattt acccaagatt ttggaccaaa tttttcattt
     1321 gtaattttaa ttacaacaat cctttttata ggggctcaag taaatgtatg gagagtaatt
     1381 ggtgtatcag gacttagagg tcaggatata gcaaataaaa taatacctgg attaggttat
     1441 ttagtagctt ttttagtagc tcttggagga ttagctttta atataggaaa tgttggtgga
     1501 gctgcacttg gtatgaatgt aatgtttaat atgaatatga cattaggaac tgtattaagt
     1561 ggattaatag ctatatttgt atttatgtca aaaaattcaa attctctagt cgataagata
     1621 actaaatttt tagcattagg aatgataatt atagttggat atgtagcaat atcaaatcat
     1681 cctcctgttg gagaggcagt atcaagaatg gtaaaaccag aaaatccaaa aggactgata
     1741 ttcccaataa taactctact tggaggaagt gttggaggtt atataacttt tgctggagga
     1801 catagattaa tagacggtgg aataacagga gaagaaaata taaaagaaat aactaaatct
     1861 tctcttttgg gtatattagt tgccacaatg atgagagttc ttttattttt agcaatactg
     1921 gcagtggttt caaaaggtct tcaacttgac ccagaaaatc cagctgcttc agcttttaaa
     1981 tttagtgcag gagcaatagg atataaattc tttggacttg tattatggtc tgctgcaatt
     2041 acatcagtta taggagctgc ttatacatca gtttctttct taaagacact aaatcctttt
     2101 atagataaat atgaaaaata ttttataata gcatttatag caatatcaac acttataatg
     2161 gcatttatag gaaaaccagc tactttatta attttagcag gagctttaaa tggattgata
     2221 cttccaatca cattaggaat tatgcttata gcatctaaaa gaaaagatat tgttggagat
     2281 tacaagcatc ctacatggct tttaatattt ggattaatag tagttttaat atctgcatat
     2341 acaggaataa cttctcttag cagcctaggt gctttatttg cttaatttag aagaggtgac
     2401 atttatggaa actagatact tgatatcagg ggataaggca gtggttgctg aatttggaaa
     2461 tgagatatca gaagatataa acaaaaaagt aatttcattt atgagagcaa tagaaatatc
     2521 gaatcttaaa ggtattgtaa cagaaatggt accaacatat aggtcactta tgataagtta
     2581 taatccatta gaaatagatt ttgatagttt gattgagaat ttaaaaaaaa tagaagataa
     2641 tttggaaagt atagtattac caaaacctaa aatacatgag attccagtat gttatgatga
     2701 ggtttttggt atagatatta aaaacgttgc aagctacaat aatctcactg tagatgaagt
     2761 tataaaaata cacacaagca gagagtactt gatatatatg ctagggttta ctcctggatt
     2821 tccatatcta ggtggtatgg atgaaagaat agctacacct agattagaag tgccaaggac
     2881 taaaatttat ggtgggagtg ttggaatagc aggttcccag actggtgtat atcctattga
     2941 tagtcctggt ggatggcaaa taattggtag gacaccttta aaattatatg atgaaaatag
     3001 agaagaacag attttgctta gagctggaga ttttataaaa tttgttccaa taacattaga
     3061 tgaatttata gaaatcgaaa aaaataactt aagtatttaa atatcgttat agtacaggga
     3121 gtggtatata tgggattcaa agttgaatta ggaggtttcc aaacattaat acaagacaga
     3181 ggtagagttg gctatggtca atatggtgta tctggatgtg gagcaatgga tgagtatgca
     3241 catagagtag gaaatatatt agtaggaaat tctgaagatg aggcttcttt agaagtttta
     3301 atgttaggtc ctacaattac ctttgatgaa tatacgcaaa tagcagtaac tgggggagat
     3361 ttaggggcta aaataaatgg aaaagaaatt caaaactgga ggtcatatca aataaatcca
     3421 ggtgatgtat tatcatttag aggtgtgaaa tcaggtgcta gagcctatgt gtcaatagct
     3481 ggtggaatag atgttccact tgcaatggga agtaagtcaa cttatacaag agctaaaatt
     3541 ggtggatttg aaggtagagc acttaaaaaa ggcgattata taaatacttt tattcaggaa
     3601 aaagatttta caataaataa aaaattaagt tcaaaatata ttccaactta tagttcagaa
     3661 atcgtactaa gaattgtaaa aggtcctcaa tttgatgctt tttctaacgg tgaagtagag
     3721 aaatttttat caaataaata caaagttaca aatgaaatag atagaatggg ttgtagacta
     3781 gatggagaga gtataaagca ccttaatgga gcagatatta tatctgatgg tatttcttat
     3841 ggagctatac aagtacctgg tcatggaaaa ccgattataa tgttatcaga tagacagact
     3901 agtggtggct atactaagat agggaacgtt ataagtgtag atttgtataa gttagcacaa
     3961 gcaaagccaa atgatgtagt aaaatttgaa cttgtagata tatatgaagc acatagacta
     4021 ttgagagagc aagaagataa gattcaagat atctacaaga gtatgaaaaa tataagagtt
     4081 gtaaaggcaa aagtattaaa tgatatagca gtttaattta attttaacaa atacaatttt
     4141 tattataatt tataatataa cttacaaata gctatttact atttaccata attaagatgg
     4201 tttagattag tcaaatagct atttttttag tttgagtaaa tattttaaat ttaaataaat
     4261 acttcaaatt taaaataaaa gcaatattta aatctattat attatctaca aaaagtaggt
     4321 gtgtcatatt gagtttattt agattttatt ggccaaattg gaggtgatat aactccatat
     4381 ccacgagcta ttgtatcttt acttggattg atgattttat gtggtacatt agcttttata
     4441 aatgttgagt cacctggatt taatctaact accttttcac ccatacaaat atcaaccaca
     4501 ccactcgtta ccaccataat ttcttctgat gcatgatata tataatcttt ggcatcctca
     4561 ctttctggtt taagttcaaa ctctacaaat aaaattgaag gtgtaaactc agttgtaggc
     4621 attggagata ctatctcgta aaacatttca ctctttggaa atttcatcat aactctatct
     4681 tcttttctaa tgactaaatc atcagtatta ttgtcatcca taaacatata taaaggagtg
     4741 tctaaaactt cagcgatttt tctcaatgaa gacaaggatg gttcaatagc atctctttct
     4801 atttgagaga tataacttat agataaatct gtcaatttgg aaacatcact taatgttttt
     4861 ttcttgcttt ttcttatatt tctcatcttt tcacctagca taagaacctc caaataataa
     4921 aattaatata aaatattcgc ttaaaacaaa gtaattatag cacaacatag aagtagtggc
     4981 aataagaata catagtagat tttgacaatt aaaatttaat taaatccaaa taaaagtcta
     5041 tgaaaatgaa ttattgttaa attaatacaa agaaaataat aataaaaaaa tttaatagaa
     5101 gagtattaat tgaattaaga ttatcacatt atattttgga aaagagtaaa ttttcagtaa
     5161 attatcttga catatatatt gaagtaataa tataatttaa agtataaaat tttaaaaatt
     5221 gaattataac ttcaaaaaat aaggagggta atatgaagat aacagatatc aaatttgaaa
     5281 agcttagaat taaattaaaa aaacctgtag tagtttcatt tggagtaatt gaatatggag
     5341 aaagtgtaat tttaaaaatt gaaactgatg aagggtattg tggatttggc gaagcagcac
     5401 ctcttgcagc agttacaggt gaagttttgg ataatgttct ttcagtattg cttatgttta
     5461 aaaaagagtt aataggcaaa gacccactag atattgaaac catacatacc atcatggatg
     5521 gaattattat aggtaatact tctgcaaaag cagctattga catagcttta tatgatatta
     5581 aaggaaaaat tatgaatgca cccctttata aagttcttgg aggatttgat agtaaagtcc
     5641 aaacggatat tactataagt atagataagc cagaaaaaat ggcagtagaa gctttagaaa
     5701 gagtaaaaga aggttttagg atattgaagc ttaaagcagg tataaatcct gaagacgata
     5761 ttgaagctgt aaagttaatt agagaagccg ttggagagag tataagaatt agaattgatg
     5821 caaatcaagg atggaacgta aatagcagta taaatactat caaaaagtta gaagaattta
     5881 atgttgatgc aattgaacaa gcacttccac attgggattt agatggtact gcttacataa
     5941 gaaataaaag taacacaaaa attatgatag atgaatcatt acattcacca atagatgcaa
     6001 ttaaggctat aaaaaagaat gcagttgata catttaatat taagctgatg aaatcaagtg
     6061 gaatatatcc tgctataaaa atcaacaaca tagctgaggc aagtggagta aattgtatgt
     6121 taggatgtat gctggaaacg agaattggta taacagcagc agcaaattta atagcatcta
     6181 aaaagaacat aacagaagct gaccttgata gttttatgtt ttgtgaagaa ttagaaggta
     6241 tatctggagg atttgtaatg gatggagata ttatgaatct tgtcaataaa ccaggtttag
     6301 gaatagaagt aaacttatga taaatttgga ggttaaatat gaatacactt ttttcaaatc
     6361 caggtagtat acttgctgta atgacatcaa tgattgcatt gggtttttac ttacaaagat
     6421 acaaagccat taaaagctta ggaccagcac ttacaataat cataatggga ataatacttt
     6481 caaacttaaa agtagttcca gtaagtactg agttatatgg aactatttca acatatgcta
     6541 ttccagtttc tatgactata atgttgatga gtgttgactt aaaagaaatg acaaaattat
     6601 caagagaacc tttaattgca atttttgtag ctgtattaac tgtaagtatc atggcatttt
     6661 tatttggatt agtatttgca gaaaaaatat cagaaggttg gaaggttgct ggaatgtttg
     6721 taggtacata cactggtgga agtgctaatc ttacagctat tggaacaggt cttaatgtaa
     6781 gtagacaaac tcttgcagca gcaaatgcag cagactatgt aattggagta ccaacattga
     6841 tatttatgtt tgctctacca gcaatattaa aaaattcaaa gaagtttaaa aaactttggc
     6901 catatcatgt agaagaatca gaattagaag attgtcaaaa tgaagaattt atggagtcaa
     6961 aagaatggag tattaaagat atagcttgga tgttggctat aggttttgtt gttacagaag
     7021 tttcaacaat acttgctgga tatttcaatt caagttttag tagtgcagca agaatacttt
     7081 tagttacaac aatttcaatt ataattgctc aattaaagcc agtaaagaaa ctaaagggaa
     7141 atttagattt aggattattt gttgcattat tctttttatg tacaataggt ttttcagtag
     7201 atataaaaga gtttttaggt tcaactttta ctataacact ttactgtttt agcataattt
     7261 ttgcttcatt tgtttttcat ttaggcataa ctagactttt aaagataaag tatcaatatg
     7321 taatactttc tatagtagga gcaattgctg atggtcctac ttcagcttta gtagctgctt
     7381 ctgcaaagtg gaattcgctt gtaagtgtag cagtcgtaat gggagtaatt ggtggagtat
     7441 taggaaatta tgcaggtata tcagtggcat atgcaataaa tatgtctcta ggattatagg
     7501 ggggagttta attgataaaa tttaaattat acatatctgg actatacagt ggaaatgtaa
     7561 tctttgatgg agatttatta attgaaaaat tgaatccttt tacaaataaa atagaaagtt
     7621 taaaaccaat aagcaaagaa gaaaatacat actatttaaa tcttacaaaa atagatttaa
     7681 agtctttatt taataatttt gatgtatata ctaaaagctt agtaaacaca gataaagaca
     7741 cagttataaa taacttagga gaaacgcatt ctaaacttaa tgaatatata tgggttcaaa
     7801 gaaacaaaaa gttcccacta gatataatta ttgtagataa taaaatagta ggttttatct
     7861 gtttatccag agaaacttgt actatattaa ttatggatgg atatgaagag tatactgtat
     7921 taaaagaatg ggaaaaaact cataaaaatg aagagatata ttctataaga tttggtggaa
     7981 attatatgat tgatatgaaa gatggtataa aactttctac agatgtatat ctccctgatt
     8041 ttgtagattc tgccaaaaaa gcaccgacaa ttcttatgag aactccatat ggaaaagaaa
     8101 atgataaaga aatatattat aaatatgtac aaagaggata tgctgttgta atacaagatg
     8161 ttaggggaag aaatgaatca gaaggtaagt gggaacctat gattcatgag agggaagatg
     8221 gagatagtac cataaattgg atagtatcac aggaatggtc aagtggaata gttggaatgc
     8281 ttggagcatc atatcttggt tatgtccaat gggcagctgc atcttcaggc aataagcatc
     8341 ttaaagcttt agttagtata gtaacatcag gaagtccttt tattgatata ccaagaaaag
     8401 gaggagcttt tgtatctggt atgcttgcat gggcatttat ggtatcaaga aataaagttg
     8461 atagaagcaa aatggtaaga gatgattggg atgatgtttt aaacatacga ccaatagaga
     8521 atatacctgt tgaagctctt ggatatagaa ttgaatttct ggaagaatgg ttaaagagag
     8581 tagaaaaaga tgagtattgg gatttgatgg attggcattt acaaaaagat aaaataaatg
     8641 tacctgcatt ggttgtatct gggtggtatg atgataatag tatgggaact acagaggcct
     8701 tagatgttat aaaggactat gaaaaaggaa agagaaaggc tattctaggt ccatggatgc
     8761 ataattcaaa tactttaaga gatataaatg gtatttcttt aggaaatagt tcattaagat
     8821 atgatttgga ttataattat ctattatggt ttgataaata cttaaaaggt atagaaaata
     8881 acatagatac gactgctcca gtagagtatt actctgtagg ttctaataag tggaagacag
     8941 aagaaaattg gccaataata aataagatag ataaaagtat gtatctaata agtgatggaa
     9001 atgctaacac atctttagga aatggtagat tagtatttga taatgattta gaagaaaagt
     9061 atgatagtta tatatataat ccaaaagacc catcagtaca actaattgat atgtcagaga
     9121 atgaagttgg agttccaaat aactacaaag atttagaaaa aagaagtgat atgttgtgtt
     9181 atacatcaga tgcttttagt gaagaattta ctgtaacagg tgatataaag ttagagtttt
     9241 ttgcatcaag ttctgccaaa gatactgact gggttataaa aattatggac gtggatttag
     9301 atggaaattc aattaaatta gctgatggta tacttagtgc tagatttaga aatagttttt
     9361 ataagtcaga gtttatggaa gaaggagaga tttataagtt tacggttata acttcaaaaa
     9421 tatcaaacac gtttaaagtt ggtcataaaa ttagactaga tataacatct agtgctaaaa
     9481 actttatttt ccaaaatagc aacacgacag agggatataa tagtattgaa tatatagaag
     9541 caaagaatac gatttaccat ggaggtaaat atccatctaa attgatattg ccaatagaga
     9601 ataagtaaat ttatatagat taaatagtga ggaggtgtct caaaatgaat tttttagttc
     9661 gtgagacact ttcttttata cgaaatcaaa tatattttca tcatttcaac aatgaaaaag
     9721 aggcttatct ctctgttttg agatagcctc ctataagcta aattgattat tttgtagctg
     9781 caacaacgct catttcaact aacaaatgct ctctagcaag tctagcttca acacaagctc
     9841 tagcaggttc aaaccctttt tcaacccaag catcccaaac agagttcata gcttcaaagt
     9901 ctttcatatc tcttaaataa atagtaacag atagcaaatg ttgcttatct gaaccatatt
     9961 tgtttaataa atcttctatt ttagctaaaa cttcagttgt ttgttcaatt actcctcctt
    10021 cagcatgagt ttgtccacat aaataaactg tattgttatg aactacagtt ctactcattc
    10081 ttccagttcc ttcatatctc tttatttcca tattttaaat acctccatag ttaaaaaatc
    10141 tattaaaaat ctaaataata aattaaaaaa agtctatact atataaaatt atattatata
    10201 ttctgataat aatccataaa aacatataac tttttagcta taattattat tgttattaat
    10261 tgaaaagatt tattagttac ttgacatagt ttacactttt aattatagta taaattggaa
    10321 tatttagata attattattt aatgtataag ctaagttttt aattaatatg gctttataca
    10381 tttatataga aaagaggtac gtatgagtag cacaatcaaa tcaaatcgaa taaaaggtat
    10441 aatgtttata atagcatcag cgcttggctt tgcaatgatg tctgcattcg taaagctagc
    10501 tggagattta ccaagttttc aaaaagtgtt ctttagaaat ctggtatcag ctataatagc
    10561 tttttggctt atcataaaac ataaaggaag tctaactggt aaaaaagaaa atttaaaagt
    10621 acttttatat aggtcaatat ttggaactct aggagttata tttaactact atgcaattga
    10681 caagttgatg ctttcagatg ctaatatgct aaataaaatg agtcccttcc ttgtagtaat
    10741 attttgtgca attgctctaa aagaaaaaat aaatgtaaag cagataggag ctattataat
    10801 tgcatttata ggagctttat tcataataaa accaacattt agtgtagaag taattccata
    10861 tcttggagga attgcaggag ctgtatttgc agcaatggca tatacttgtt taagagttct
    10921 tggagataga gaagattact atacaattgt atttttcttt tcagtttttt cattagttac
    10981 tgtagggcca attgcttttg cagtatatga accaatgacc cttatgcaac ttatatatct
    11041 attactaggt ggagtatttg caagtctagg tcaatttgga ataactctag catataaata
    11101 tgcacctgct aaggaaatct ctatatttga ctatagtaat ataatctttt cagcaatttt
    11161 aagtatattc ttattcaatg tttatccaga tatgttaagt gttgttggat atttaatagt
    11221 attttcggca gcattttata tgtttttata caataagaag ttagataaat tagacaaaga
    11281 aaagaagaag taaactagat aaaagtgttt ttataatttg tattgaatat ttaagtcttt
    11341 gtattaaaat taaagaaaat ttaattaaaa atgtttggta tattaactta taacagcagt
    11401 taaaattgaa aaattataac tgctgtttct tttattaaaa atatccaaat gtctgtaaaa
    11461 atagactata atagagtagt ataaataata taaaggggat aaattaaagt atggaaaaag
    11521 tgaaagttgc tgtgattgga gcaggtaata gaggaacgta tgcatatgct ccatatattt
    11581 atgaaaattc tgatgtatgt gaaatagttg ctgtagcaga acctaaaaaa ggaagacgag
    11641 aactatttac acaaaaatat aacctagata gtaaaaatgt atttgaaact ttagaagact
    11701 tttttaaaca tgacaagatg gcagatgcag taataatagc tacaaatgat gatagacatt
    11761 atgatgtagc aaaactagct cttgaaaaag gttatcatgt actattagaa aagccaatgt
    11821 ctaatagttt agatggtttg gtacatatag atgacttgtg tgataagtat aaagataaga
    11881 tttttatgat ttgccatgta ctgagatatt ctccatttta taataaatta aaagaaattg
    11941 tagaaagcaa aaaattagga gagctagtta gtatacaata taatgaaaat ataggatatt
    12001 ggcattttgc acatagcttt actagaggga attggagaaa tagcaatgaa accagccctt
    12061 taattttagc aaagagttgt catgacatgg atattttgtt atatttagta ggaagtagat
    12121 gcaaaaaaat atctgcattt ggaagcttaa agcatcttaa taaccaaaat gctagtggag
    12181 agatggcaca aaattgtctt cagtgtttag ttgaaaaaaa atgtccatat tcagcaaaga
    12241 gaatttattt agaaaaagat agaagtataa atagagctgt tcacataaat ccaactgaag
    12301 agaatctatt aaatatactt aagacaagtc catatggaag atgtgtttac agatgtgata
    12361 ataatgttgt agacaatatg gtaaatatac tacagtttga aaatggagtg acggctactt
    12421 ttaatttatg tgcatttact aaagaaaatg gaagaactat aaagttaatg tttagccatg
    12481 gtgaagttgg tggggattta aataaaaatg agataagaat taaagaattt ggaaaaaacg
    12541 aagaaatagt tatgaatcca tcaaatcaac agaatatggt agaggaatat gatagaaatc
    12601 ttatagctga atttattaaa ttagtttcaa ataaagaact agaaaagggt agagtagcag
    12661 caaaagaagc tatacaaagt catgtaatgg catttgcagc agaatattct agggtcagtg
    12721 atgaagtagt gtatatagaa gaattttttg atagtgctaa gcaaatgact aaggagatag
    12781 aagaaactat attttaatta ataaaaattt attaaataaa ataaagatag attaaataat
    12841 agaaatacga aaatatgtgt tctattaagg aatctatctt ttttattaag gttttttctt
    12901 atccctatca aaaaaatcca tacattctga taaaagataa gaaagtaaag cgaataagat
    12961 ttctgtactt aaaggaatcg ttactgaatc cataaaggta tcaattatgt tcatagctag
    13021 aaaagttagt ataaaatcaa ttataaattc gcaaagttta tatattgagt atggtaattt
    13081 gtttacatat ctgaatatat ctaaaatagt agtgcataga aaatcaattg gagtggttat
    13141 acaaaaatat attaaaaaga aaattaatac agctccaaga gatttatatt caaatcctaa
    13201 gaatgataag aagccaattc ctataaccgt gaaaatacaa aaagttatac caaaaaaaac
    13261 agcaaaaaat aaaaatacag aaataaattc gcctaaagga atccttcttt tattacttat
    13321 cttaatcaac tccttaattt atgatatgaa atttatataa ataataacat tcttaaaaat
    13381 tcataatagt cctctgtttc aaatagtact gtttgagaat ctattttttt catatttgga
    13441 taaaaagagg ccttaaaagc tttataatgt tctctaaatt ttatttctat cttaaattta
    13501 tctggcaatt ttattaagtg cctagataaa tctccagata aagctttttt gacacctttt
    13561 tcaatttttt ttagtgcaag gtttggatgg attgaaattg aaccatttcc aattcctttt
    13621 gacacaggaa cagtgactat atttggattt agtttttttg cactctcaca aagcatttca
    13681 tctccactta aaaatgctac aggaacatta taatatgcag caatataagc atttatagta
    13741 aattcagatg ctatttcatc atttatttta aaatagtcat aatcaatatc catagtatgg
    13801 gaaagtggat taccattaca agtagctcca gagtgatagc cagtgaatat acatgcatca
    13861 aagctttcat caattccagc catcataact agtgggtctt tggtccatcc tctaagtatt
    13921 ttgacgtttt caggtaaaat atttggatta atatttctac cactgtcatg tgcatcttta
    13981 acaagtattt catgtacacc agattgagta gctccatcac atgctgctgt tatctcttta
    14041 ctcatttgtg ttctatagtg ttgcgaaaat tcacattcaa gttttgtttc atcccaattt
    14101 acaataccac aagtaccttc tatatcagca cttaaataca atttcataat atataccccc
    14161 ttatgtttat tattgatgat aataacagat aacagaaaat acataatcaa taaagggcat
    14221 aaaaatatta tgtcttacta ttaactatta ttaaatctgt aactgaaaaa taactaaatt
    14281 ggtataaaaa aatataacac tatgtgtcat ttatttatat agttttttat atctgatgtt
    14341 tatataaaat tgtaacatat aagtggttac atttactagt ttaacatagt taaaatagtt
    14401 aggtattatt tttaagttaa attgattatt ataaaatcgc agaataaaag aaaaatttaa
    14461 tttattaaaa tgcatgaaat gttttttcct caacataatg ctaaattaaa ctcaaaaaga
    14521 aataatagaa aaatcttatg tagcttagaa agaatgtgaa tgatttaagg gaaaaataga
    14581 aatttattat ataatattag gctgtattat ataatacagc ctaatattat atgtatatgt
    14641 gacataaaat cttggtaagt gtaaataaaa taaatgtgga aaacaagtta atgaaatcaa
    14701 gaaaaaagta atttaaaatt caatttcatc ttcccaatta aattctcctg atttttctac
    14761 actcttaggc cagtgactac accatttagg aacggacgga ttttcaaccc tatcaacgct
    14821 tggagtatat ggctttaagt caataacagg actattatca ttagcatcaa tatatggtat
    14881 ttttattatg ccattttcat ggtcgatatt gataatttgt acagcagtca atgctattgg
    14941 attgggtctt ataggtgacc ttgttgcaaa tattcctata acgtctggtc cacttttata
    15001 aggttttggg gtttctaaaa tacttcttag ttcaggatca tcaaacccat cagcccacca
    15061 aaatacattt aaatgactga aatcgtcgag ttcatttaat gcaggtacat actttttgtc
    15121 taatttaata aagacctctt cgccttgaat acatatttta cctataggat ttattaatat
    15181 ttctttcata atattacctc caactattta atttatcatt tgtaatttta gggtataaca
    15241 tgacatcatg tgagggtcaa tataaaataa taaataaaaa tattaatagt tagaatgata
    15301 atacattttt tacatggagc atgacaccag taataaggca atcttcgtca atatcaaaag
    15361 tagaggtatg caaaggggaa gtaatgtttt tttcacgatt tgcacaacca aggtggtaaa
    15421 aagctccttc acagtgtctt agaaaatacg aaaagtcttc agccccaaga gtgggcgagt
    15481 ctttaaggat aatattttct tcaccaaaca attctacagc attttgtttg acgtaatcta
    15541 ctagatggtt tgaattgata agagctggat aaccttcctc aatttcaaca gaaatacttc
    15601 ctccaaatgt tttacaagta aaatcgcaaa tttctctaat tcgttttttt gaaaactctc
    15661 tagtttcagg agtcaaggtt ctcaaagtac cccttataac cactttatca caaactatat
    15721 tttccttgat tccaccttca atctttccta gagaaataac cacagaatca gttggatttg
    15781 tgtttcgact aacgattgtc tgtagagctg taataatatg agcagatata acaatagcat
    15841 caataccttg atgtggataa gctccatgac attttgaacc atgtacagtt aattgaagtg
    15901 tatcggtgga agcatttaga gtgttgtatt ttaattcaat tagatttttg ttgatattgg
    15961 gatttacatg aagtccaaat atatatttta catttgggtt ttcaagacat ccatcttcta
    16021 tcataagttg agccccacca atagtttcct cggcaggttg aaaaaagaat tttacattta
    16081 catttaagta atcctttaat ttaaatagta tattacaagt acccagtaaa atagcagtgt
    16141 gtgcatcatg accacatgca tgcattttgc caatattagt agatttataa gaataattaa
    16201 gttcttctgt tataggtaag gcatctatat cagctcttat agcaactgtt tcaaagtttg
    16261 gactcacgtt aatgtatgca gttatccctg tatgattctt atattcaata taatttatac
    16321 ctatttcatt taagtattta ataatttttt tcttagtttg aaattcttct aatccaagtt
    16381 ctggagtctt atgtaaatct cttcttatat ttataaccca atcatttatc tcatgacaaa
    16441 gagtttttat attaagcata aaaaacatcc taactatatt aaaaatttat ttttcttcat
    16501 taattatatt attttatgta aatcattatt atgtatcaag tacaaataaa atatcttata
    16561 attatattag agatatttag tatggagaag aatatatcat tttataagaa gaggtgttta
    16621 aatgttagga ataattgcgc catctgggcc attaagaaat acaagtttgg aggaaataaa
    16681 gtttaattta gaaagttatg gttacgaagt taagttttct gaaagttgta gtttaaatta
    16741 taaaggttat ttagctggta atgatgatat tagagcaagg gatatagaag atatgttttt
    16801 agataaagat gtagatataa ttatgtgctt aagaggaggt tatggaacaa caagaatatt
    16861 ggataaaata aattatgata taataaagca aaatccaaaa ccatttatag gtttttctga
    16921 tataacaggt ttaaatttag ctttctataa aaattgtggt cttttaccat atcatggaat
    16981 aatggcagca aatgtaggta aatgggataa atttacctat aaatcattgg taaatgcttt
    17041 agaatttaaa gatgaattat atttagaaaa tccaaaagaa gaaaagattt atactgtatg
    17101 tgaaggtaaa gcagagggaa taataatggg aggaaattta tccttaataa ttgccacaat
    17161 gggaacaaaa tatgaaatag atacaaaaga taaaattttg tttattgagg aaattggaga
    17221 acctcaatat aaattggata gaatgttaac acaactatat tcttcaggaa aacttgagga
    17281 atgtaatggt ataatttttg gagattttaa ggattgtatt gaagaaaatg atttgatgga
    17341 attactaata gaatttgcaa ataaggtcaa taaaccttct atatataatc ttcagtcagg
    17401 acattgtatc ccaatgataa ctattccttt gggcagaatg tgcgaattag atgctacaga
    17461 aaaaatcgtt aaattaaaaa aatagtgtgt atataaatat aatagaaata tggtataatc
    17521 ttatacaagt aaaaatttag ctaggaggat gaaaaatgtc tgatgataga ataatagact
    17581 ttaacgaact aaagaacaaa gttaaagatt ctgatgtaga taaatttgag caatacatat
    17641 ataacttata tttttctgtt atggatggaa agatgtctat ggctgagttt tcaagaaaaa
    17701 tattcgatta tatgagagat aacaacatat ctcaagaaaa atttatgaaa atacaaaagc
    17761 aatttatgga gagatatggt atggacactg aagaagtaga aaaacaactt agaaactttg
    17821 gaatcgaccc ttctacagca ggttttatga gtaataatac ttccagcaaa gtatctacag
    17881 aagatttaga gtcatttaag aagagtgcag gattttatga gaaatatgga gaaaaaatcc
    17941 aaccaaagag ttgtataaca acatttataa aaaatgactt aaatgatatt aatgtaataa
    18001 tcgaccaaga aaagataatg ctttgtagtg atagaaaaat aaatcttatg gattcagaac
    18061 taaatgagtt tttattagaa tataagaata tgtttaataa aaaaataaag gtagtaatgt
    18121 gcgaaactac gaataagtat gattattaaa aaatgaattt aaatactgaa gtagtataaa
    18181 ttaattgtac tacttaaaaa taaaaatcgc aatttattat tccttataaa tttgtataag
    18241 gaatatctaa actgcgattt tttatattgt gaagattagt cataatagat agtgttttaa
    18301 attgtaatct tctcactaaa actgtgtttt tctattagaa attttattaa aaataaatat
    18361 ctgataattg tattatatgg gatgtaaagt attagaggct taacgaatct cttttaatac
    18421 attaactcca ttttgaatca tactgttgaa aaatactaag tttaaaatct cagcagggtg
    18481 aacgttagga atatcataag tatctactaa attcataggt acaactatat ttttttcaat
    18541 attatgttga ttaaaatatg attttagagt tattgcaaat tgatatatac atatatctgt
    18601 gcaatcgcca actataatta tattatctat attatcaaaa tccaaatttt ctaatgcaaa
    18661 aaaaccatta gtagaatttt taggaagaat ttgtaggttt tctatcgact taagttcatc
    18721 aaccaattca ctttctacat cattttccaa acagtgaaca ggataactca aaagttctat
    18781 tgatttagga gtatgagaat cagtaaatgc tattacttta tttaatttgt ttgatatttt
    18841 ttttgtaaac atttcaattg gctttattaa agactctact ctaggagagt ataatgcacc
    18901 ttgtcttgca aagccattat taacatcaat tataaataaa gcagttttac tcaaatcata
    18961 attttctatt ttttctattg gtagattatc taaattactc tttaaagctt caagttcatt
    19021 taataagtta tccatagtta tacctccaag ataaatttaa taaaattgta catattaatt
    19081 atataaaagt caaggagtac aaactattgt ataattagct gtattgtttt gagtgtaaaa
    19141 gatttgaagc aatatctgga tagttagtaa aaataccatc tacattgagt tttagacagt
    19201 gtagtatatc atcctcttca tttacagtat atacattgac accaagtaga tttttgtgac
    19261 aaagttcaat tagagattca tttactaaaa ctagaggtgg atgaaggtaa ctattaccca
    19321 gaaatttagc aaaattgact acattgttta taggtaaata acacaataat cctgttttta
    19381 tactagggtc taatttatga aagtttttta agctattgct gttgaaacta gatataagaa
    19441 ctctattttt catattatac tttaaaataa gctctaaaac gtctttttct atattgggat
    19501 agtttatttt gtcagttttt agttctatat ttaaaataag gtctgtaggt gaaattagaa
    19561 ttaatacttc ttctaaagtt ggaattttac attctttatt gctttgatat ccttcaaaag
    19621 atgagtttag agtttttaat tttcttaaag taaagtcttt tacaaaacca tgtccattaa
    19681 aagttctatc aaccttttca tcatgaataa ctacaatttt tccatcctta gttctgtgga
    19741 catcaagttc tattccatat atatccatat ccaaacattt tttaaaagct agaatagtat
    19801 tttctggata ctttccacta aaccccctat gagcataaat attcatttat atggttccct
    19861 cctataagta taacttatct tataaaaatt taattaatat aatatatcat attccaaaag
    19921 gtattgttat atattagtta aataactatt tgtaatttat atattatata aatgtagaaa
    19981 aataaaatca tatatgtgtt ataatatatt ttaaacattg taaatattac gaatttagta
    20041 gtcataataa gtgtaatttt atgcaagctc atgtttttag agcttgcata tgtattattc
    20101 atatatttat atattgtgaa aataaaattc caaaagaatt ttttctatag acttagtagt
    20161 aagggggaat tttgatatgt atgaagttaa tttagaacga gctatgaatt attggatgga
    20221 tttgttagat gattatacga atatagcaaa aattccaaat gatttttttt ataaagattg
    20281 ttctgatgaa gtagtagatg aggtttttaa gatgaatatt gagaaacatg aaaggttaat
    20341 tcaatattct aaagtgaaca atattagcat agactctatc atagaaacta tatatggcct
    20401 tatacttcaa aagtatatat atgaagatga tgtagttttt ggtaaaacaa tgaatataat
    20461 tccaataaga gtaagagtta gacaaagtga aaaatttata gatgttgtaa ggaagctaaa
    20521 aaggcagtgg agcaggtctt tagagtatgg atactatttg ttatcagata tagagaataa
    20581 aaatcatcta aatactaagc ttataaacac aatatttata gctaaaagtt ttgatttatc
    20641 atatgattat gacatagaaa aaaaaggata tgatttatct gtaatagtat atactaagag
    20701 ggcacttaaa ataaaaataa agtataaacc atctatatat aaaagagaaa ctattagaag
    20761 aattttaaat caatttgatt atataataga gcaaataata gaagataatg atattttaat
    20821 agaagattta gaatttgtat gtaaggaaga agaaaaagta ttattggaag actttaataa
    20881 caatcaatat gaaataccct atgataaaag ttatgttgat ttatttaggg agcaagttaa
    20941 ggtaacacct agtaatatag ctgctaggga tgaaaataga aagttaacat ataaagaatt
    21001 ggatgaagta actgataaac ttgcaggtta tttaaatcat ataggagtgg aaagtgaaga
    21061 catagtagct gtaatgttac caagagatat taacataatt attacagcaa tagggattat
    21121 gaaatcaggg ggagcatttt ttccaataga cacaagtaat ccagaagaaa gattgaatta
    21181 ccttcttgaa gatagtaatg caaaagtagt gataactaca gatgaactca aaagtaaggt
    21241 tgtaaatgag aataccatag tactcgatat aaatgatgaa gaaatgttta agcttggata
    21301 tgaattaact gaaaaaataa catcatcaaa ttgtgcatat acgatatcta cttcagggtc
    21361 aactggtagg ccaaagacca tagctataga acataagagt ttagtaaata tgtgttatta
    21421 tagcgtaaaa tctataagcg ctacagaaaa tgatatatgt ggtatatact taagtttcag
    21481 ttttgatgct gtaatgaaac aactattccc ttatttgctt gtaggtgcat caatagatat
    21541 tatgcctgaa gaagctaaat ttgatgaata tacagtaaat gaatactgtg aggaaaatga
    21601 cataactata ctagcattgc caacagcatt tgcaaggtta tttatacaaa attgcaataa
    21661 taattcactt agagttgtac agaccggagg agaaagatta aaaggatata gaaagcgtaa
    21721 ttatgaacta tataatgagt atggaccaac cgaatttact gttgtatcga cttcttttca
    21781 tgtggacaga gaatatggaa aaataccaat aggaaaacct atatttaata cttatgcata
    21841 tgtattagat aaaaagaaca aactatgtcc aattggagca ccaggagagc tatgtctatc
    21901 aggcattcaa atatcaagag gatatttaaa taaaaaaggg ctaacagagc aagtatttgt
    21961 agagaatcct tacaaaactt gtgaacataa taagttaatg tatagaactg gtgatttagt
    22021 aagatggtta gatgatggaa atttagatta tattggaagg atggacaatc aagtaaaaat
    22081 tgatgagttt aggatagagc tatatgagat tgaaaatata ataaataata ttacagaaat
    22141 taaaagtgtt gtttgtatat cgagaacaaa tgatgatgga gatatgtata tatgtgcata
    22201 ttatgtaatt gatgaggaag atagtggtaa gataaatgaa agaactatta gagaatattt
    22261 aaatgagcat ctacctccat atatgattcc aacaataata atgaggatag ataagatacc
    22321 agttacacca atcggaaaag taaataaaag agcattacca gaataatttt ttattatgat
    22381 aggtgtcata aagtgatttc agacatttct ttatgacact tatttttata aaagaatcaa
    22441 tttgcataaa aacaagaatt ttctgtatat atttactatg gtgaaaattt atttataaga
    22501 atgattattg tattttttat acttttccgt
//
LOCUS       DAMJYC010000059        22174 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJYC010000059 DAMJYC010000000
VERSION     DAMJYC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.060"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            88..429
                     /locus_tag="PC472_002492"
     CDS             88..429
                     /locus_tag="PC472_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2727809.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESANYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            580..993
                     /locus_tag="PC472_002493"
     CDS             580..993
                     /locus_tag="PC472_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2727810.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1195..1836
                     /locus_tag="PC472_002494"
     CDS             1195..1836
                     /locus_tag="PC472_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2727811.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1860..3113
                     /locus_tag="PC472_002495"
     CDS             1860..3113
                     /locus_tag="PC472_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2727812.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3159..3809
                     /locus_tag="PC472_002496"
     CDS             3159..3809
                     /locus_tag="PC472_002496"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2727813.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3860..4456
                     /locus_tag="PC472_002497"
     CDS             3860..4456
                     /locus_tag="PC472_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2727814.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4469..5308
                     /locus_tag="PC472_002498"
     CDS             4469..5308
                     /locus_tag="PC472_002498"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2727815.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5553..6707
                     /locus_tag="PC472_002499"
     CDS             5553..6707
                     /locus_tag="PC472_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2727816.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7013..8710
                     /gene="ade"
                     /locus_tag="PC472_002500"
     CDS             7013..8710
                     /gene="ade"
                     /locus_tag="PC472_002500"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2727817.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            9062..9526
                     /gene="bcp"
                     /locus_tag="PC472_002501"
     CDS             9062..9526
                     /gene="bcp"
                     /locus_tag="PC472_002501"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2727818.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            9536..10279
                     /gene="yaaA"
                     /locus_tag="PC472_002502"
     CDS             9536..10279
                     /gene="yaaA"
                     /locus_tag="PC472_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2727819.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFAIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            10407..13064
                     /locus_tag="PC472_002503"
     CDS             10407..13064
                     /locus_tag="PC472_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2727820.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVAGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      13118..13220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13330..14595
                     /locus_tag="PC472_002504"
     CDS             13330..14595
                     /locus_tag="PC472_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2727821.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14625..15515
                     /gene="metA"
                     /locus_tag="PC472_002505"
     CDS             14625..15515
                     /gene="metA"
                     /locus_tag="PC472_002505"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2727822.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            16006..16449
                     /locus_tag="PC472_002506"
     CDS             16006..16449
                     /locus_tag="PC472_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2727823.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            16480..18297
                     /gene="ftsH"
                     /locus_tag="PC472_002507"
     CDS             16480..18297
                     /gene="ftsH"
                     /locus_tag="PC472_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2727824.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            18475..21177
                     /locus_tag="PC472_002508"
     CDS             18475..21177
                     /locus_tag="PC472_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2727825.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            21211..21909
                     /locus_tag="PC472_002509"
     CDS             21211..21909
                     /locus_tag="PC472_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727826.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKETWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         8856 a         2382 c         3987 g         6949 t
ORIGIN      
        1 gtaaatcagt cttacatata tttccgatag attatattga taaaaaacac taaaattaaa
       61 ttaataaaag tacttggagg gatggaaatg aaagaggaaa attttattaa tgttgaatac
      121 gaggataaac gtttttcaat taatagtaaa gaagttttgt acatagaaaa gcaagagtat
      181 gaagtaaaga tttatactga aagtgctaat tatgaagttg gagaaaatgc tccttgtata
      241 agaaaactat tgaaagaaga aaattttttc aaatgcagta atagttatat tgtaaatcta
      301 gacaagtgtg aattttatct cagcaataat tgtatttttg tgaataataa ttttataaga
      361 attttaggtt cagaaagaaa aaagaaatta aaaaagaaat tagaaaaatt atatgattat
      421 gaagattaat attgggaggc tgtattttat acagcctctt tatttattga ttgtattatt
      481 aataaatttt aaattatgat aaaatataaa acaagtaact tatttcagaa taaaatactt
      541 tttaaaagaa agggacaaga tatgggagat tataataaaa tgattgaatt aaagaataac
      601 ttaggtatca gtgaaagaaa gttaaaatat ccttgtatct ataaacattt taaagggaaa
      661 tattatgcta caatgggtct tagcaaggct attgatgata tagaaaacat atgtgaaata
      721 tatggtaaag aaaatttaat ccaaaacaga aataaatata aattagttat tagacatact
      781 gaaagagaag aagatatcta tgtttatagg gatttagatg gaaactttta tcataaaaaa
      841 gaagaagata ctaatgattt agttttatac aagaccttat atgatgatac aggaatattt
      901 gcaagacctt tggatatgtt tttagaaaaa gtagatacag ataagtatgt caattcaatt
      961 caagaataca gatttgaaga agtatataaa taatataaga gaaatatgct tataaattta
     1021 gaaaaatttt ttaacagtac aataatttgc agaaatacaa atgagattta ttgtaaataa
     1081 tatgttagat tagattataa aatagaaaca aagtaaagta ttatatgata taattaagtt
     1141 ttgagaagtg ataatttata tttaataaaa aataggcata tattaggagg acatatggga
     1201 aatacttttt atatagtaag acatggacaa acggattgga atatattggg aaaaacacaa
     1261 ggtcatggta attctgacct tacgcctcaa ggtatagaac aggcaaaaga actttctgaa
     1321 gatattggca agtattcaat tgattatatt tttagtagcg accttgggag agcaatgcaa
     1381 acagctcaaa ttttaggaga taagctcaat attgaagttc aaaaaactga ggcacttaga
     1441 gaaatgggat tcggagtttg ggaaggcctt ttaataaaag aaattcaaaa agattattca
     1501 gatatatatg caacatggag aaatgaacca cacttagtaa atataccaga aggcgaaact
     1561 cttaagatta taaaggaaag agtagatgct tttataaagg aactaaatga aaaatatgat
     1621 aataaaaata taattcttgt tacacattca attacattga gggttatgtt gttgtcgttt
     1681 ttagaatcag gtatggaaaa tatatataga attaagcaag ataatactgc tttaaatata
     1741 gtagaattta aagattatgg acctgttata gtaaaaatga atgatacaag tcatataaaa
     1801 aatcatgtaa agataaataa ttctgcactg gagtaggaat caattataag gagaaattta
     1861 tgagtaaagt tatagtagtt ggtggaggag cttctggaat gatggcagct ttgtcagctt
     1921 caaaaaacaa taatgaagta atattagttg agagaaatgg agaattagga agaaaactta
     1981 gggcaactgg tggcggtaga tgcaatttta ccaataatag agagatagaa gatttttttg
     2041 ataaagttgt cagtaataaa aaatttctct atagtagttt ctatacattt acaaataaag
     2101 accttatttc gtattttgaa agtagaaatt tggaatataa gattgaagaa gaaaatgacc
     2161 acaaagtata cactaaaaat gataaatcta tagaagtaat agaagtatta aataaagatt
     2221 tactcaatca caatgtaaag ataatgtata ataagaaagt tatagatatt ataactgaag
     2281 aaattgctct aaaagatgat tctaataagg acaagtcaaa gtatctaata aaagggatta
     2341 ttttagataa tggagataag atactaggag acaaggttat tatatcaact ggaggagtga
     2401 gttattcaaa gacgggttca gatggttcaa tgtataagat tcttaaaaag catggacata
     2461 cattaaacaa actatatcct gctttagttc cacttacaat agaggaaaaa tggattaaag
     2521 atttacaagg aatatccatg aagaatgtgg aaatatcttg taagattaag aaaaggaaaa
     2581 tatcaaaatc tggagacatg ctatttgccc attttgggat aacagggcct tgtgttctta
     2641 taatgtcttc ttatattaac aagattattg aaaaggaaaa ggtagaatta aatatagatt
     2701 tcttaccaaa tttaagtact gatgaaatct ctagtataat cagagcattt ccaaataaaa
     2761 atgttctaaa taatcttaag caaatactac ctcaaaattt cttaaaagag attttttctc
     2821 tattatcttt agttgataaa aaggcaagtg atttgtctaa ggcagatgaa attagaatta
     2881 ttgagtatat aaaaaatatg aagctaactt gtaatgggac tacaggtata aacactggta
     2941 tggttacttc tggtggtatt tcagttaaag aaattaattc ttctactatg gaatctaaat
     3001 tagttaagaa cttatttttt acaggtgaag taattgatat agatgcagaa actggtggat
     3061 ataatttaca aatagcattt tcaactggat atcttgcggg tataagtgta taaatataat
     3121 tttttaataa ttaaatatga ataaatagag gtgtatatat gggtaattta gttatagctg
     3181 tagatggtcc agcaggagct ggaaaaagta caatagccaa gatagtagca aaaaagctaa
     3241 atataaatta tatagataca ggtgctatgt acagagcagt tacttataag tgtttaaaaa
     3301 gtggtattga tgtcaataat gaaaaagaag ttattcaaat agctgaaaat tcggatatag
     3361 attttaaaga taacaatata tatttggaca aagaagttat aaatgaagaa ataagaacaa
     3421 tagaagtaag caataatgtt tctaatgtag caaaaataaa agaagtaaga cagttgatgg
     3481 ttgaagttca acgaaaaata ggaatgaaaa gttcagtaat actagatggt agagatatag
     3541 gttcatatgt tttcccagat gcagattaca aattcttttt agtagctact ccagaagaaa
     3601 gaggaaatag acgctataaa gaactttgta ataaaggata taatactact ttagaagaag
     3661 taattgagga tataataaga cgagatgaaa ttgattcaaa tagagaattt gcaccattgg
     3721 taaaagctaa tgatgcacta gaaatagata ctacaggaaa aactatagaa gaagttgtag
     3781 aagaagttgt atctaaaata aatctttagt tttttatgca tcataatgaa aataaatgta
     3841 gtgttgaagg gagataaacg tgaattttta tagatttgta ataaatattt ttaagggttt
     3901 ttctaaaata ttttttaaat atgaagttat aggtgcagaa aatattccag atagaggaaa
     3961 tatagtaata gcatcaaatc ataaatcgaa tcttgaccct atattcttgg ctgctgccat
     4021 agaaaataga gaaatagctg caattgcaaa gaaagaattg tttaaagtaa aaccattggg
     4081 atttatatta aaaaaattgc atgttatgcc tataaataga gaaaaaccag atgtatctac
     4141 aataaaaact attttaaggt cagttagaga tggttatgta ttaggaatat ttccagaggg
     4201 tactagaata aaaggagatt cttttggtaa ggctaaggca ggtctttctg tatttacaat
     4261 aaaaagtaaa tctaaagttg ttccagtatc aataatatct aaatataaac tttttagtaa
     4321 agtaattgta tatattggag aaccaatatc atttgaagaa cattttaagg aaaaattgag
     4381 taatgatgac catgaaagga tatcacaaga gattttagaa gtgataaaac aaaattactt
     4441 taaatattca aaataagggg gtaaagttat gaacataaaa atagcaaaaa atgctggctt
     4501 ttgttttgga gtaaaaagag caatgaaaat ggcatgggat gaagttgaaa aaaatgattc
     4561 tggcatatat gctttaggtc ctcttattca taacaagcaa gcagtagcaa aatatgaaga
     4621 aaaaggtctt aagactgtga atgaaattga tacaatacca aatcatgaaa atatgataat
     4681 aaggtctcat ggagttcctg aaaatatata taaggaagcc aaagataaaa aattaaaaat
     4741 tgtggatact acatgtccat ttgtaaagaa aatacataca gtagtaagtg agtatcataa
     4801 taaaggctat gaaataatag ttattggaga tatgaagcat ccagaagtta taggtataaa
     4861 tggatggtgt gaaaattcag ctataataat aaagacctta gaacaaatgg aaaatatgga
     4921 atttgataac tccaaaaaat attgtcttgt tgcacagact acaataaatc cagagttgta
     4981 tataagtata gttaataaac tatcagataa attagaagaa atagtattta atgatacaat
     5041 ttgttcagct acaaaaacaa gacaagaatc agctaaagag ttagctaaag aagttgattg
     5101 tatgatagtt ataggaggta aacacagttc aaatactcaa aagttagtaa aggtttgtga
     5161 agacttagta ccaacttttg caatagagac taaagatgaa ttggatgtaa atacgcttaa
     5221 aaaatataaa aatttgggta ttacagcagg ggcatctaca cctaattgga taatagaaga
     5281 agttgtaaca ttccttgaaa atttataatt tgataggtaa taaaaatagg tggacattgt
     5341 tataatttat cataatatcc acctttgatt tcatattaca tgtggtaaat agataaccta
     5401 atatcaaaaa gtgtgtatct taagatgaaa aactaagagt ataagatgcg cactttttaa
     5461 tattagaaat tcaaaagaaa tattgatatt gattatcaat agaaaacaga atataatata
     5521 attgttgcaa aattagtcta ggaggtaaat taatggaaat gttgatatcc gcttctgaag
     5581 aggcgttttt acatgtaggt tctatgattg gattttttat tttgttattt ggatatatta
     5641 attataaaac tagtggaaat tttactaata tcatatcaaa aaacagaaaa tttcaaccat
     5701 taataggtgc tttaattgga gctataccag gttgtggcgg aagtttggca ataatgccat
     5761 tgtatataaa tggaaaatta agctttggag caataattgc aagtttaata gctagtatgg
     5821 gagatgcagc atttgtactt atttcatcaa atattaaaat gtattttttt gtcactatag
     5881 ttagtacaat tacaggaata ataactggac aattagtgga ttactttaaa ctagaagaaa
     5941 aattagggtt aaaaaaccgt agaaaatcag ataaatatga tataagtaaa aataactcta
     6001 aagaaaataa agaacaacat aatcatgatg aaataatttt agacactcta gctaaatctc
     6061 atggaaatac aaatagactt gcgttcataa taacacatgg aaagggatat aaaatttata
     6121 taggaattat agtaatcggt tttatattta tgtcacttgc tcactctgga cttaatcttc
     6181 ctataattga gaagttacat tcattggaag aagtaatagc agtgattgga atattatttt
     6241 caataattta tatgtggtgt tttaaaaagg tatttaaaaa ttctaatcaa aaagaggaag
     6301 aaaataaaaa gatttctttg cgagaaatgc ttatccattc tgttggagaa atatcatttg
     6361 taattacatg gatttttatt gcatatctta tttacgattt gataatatta attttaggtg
     6421 gagatgaata cttagttaaa ctagtattat caacaggtgt aataagtgta tttataggtg
     6481 caggtcttgg acttattcca ggttgtggga tacaaattgt gcttatgtca ttttatttga
     6541 agggaactat accacttggt gcagtgatag ccaactctat ttctcaagat ggagatgcac
     6601 ttttcccact tttagcaatg gataaaaaat cttctttatg ggctatgatt attactacta
     6661 taccagcaat acttgtaggt atgatagttt atgcattttt aggttaaata taattacgaa
     6721 taaagaagta ttaaaaaaat aataaagcac ataaatttat ttataagaat agtgaagatt
     6781 aaatataaaa ataaaaaatt caatatgaat taaattattg cttaaatatt aatgagaaca
     6841 tgggagataa tattattcct catgttctct ctatttaatt aaaggaatta tggaacaaat
     6901 ttcaagtgat actttctatc aaaactcata tataatatat attaataaat taaactataa
     6961 tacgtaatac agtcagtata ttgatataaa aaacttagga ggggttataa aaatgggtaa
     7021 tttaaaaaga caatgtgatg tttcaagtgg aagagaaaaa gctgacattg tactaaaaaa
     7081 tgggacaatc ataaatgtat ttacagaaga gttaataaca ggagatgtag caatagtagg
     7141 agacactatc gttggtattg gcgattacaa gggcaatgta gaaattgatt gtagcaacaa
     7201 atatatatca cctggcttta ttgatgcaca tatgcatatt gaatctacaa tggtaatgcc
     7261 aattgaatta tcgaaaaaat tattaaaatc aggtacgaca actatcatag ccgacccaca
     7321 tgagttggtc aatgtaaaag gagctagtgc aatagatttt ttattagaat cgacaaaaga
     7381 tataccactt aatgtatata ttatggtgcc ttcatccgta cctgcaacaa gttttgaaac
     7441 taatggagtt ggtaaattta gtgcaaaaga catggagagt tatgtaaata atcctagaat
     7501 tttaggttta ggagaggtaa tgtgttttaa tgatgttata aattctgaaa atgaaatctt
     7561 ggataaacta gagcttttta aaaataaagt tgtagatgga catgcaccta atataaatgg
     7621 taaatcactt cagacatatg tatgtgcagg aatagaaaat gaccatgaat gtataacttt
     7681 tgatgaagta tatgaaaaat tgagagcagg attaaaaatt ttaataagag aaggaagcgc
     7741 agcaaaaaat ttaaagtcta tagtaagtgg tatgctaaag cataacttgc caattgaaga
     7801 atttatgttt tgcacagatg ataagcactt agatgatata gaaaaacaag gtcacataag
     7861 atggaatata aaatgtgcga ttgatttagg tatggaacct gttagggcta taaaagttgc
     7921 aacttataat tctgcaaaag catatgggtt aaggaaaatt ggagcaatag gtgcgggtta
     7981 taaggcagat attgttgtat tgaatgattt agacaaaatg gaagtggata gtgtatataa
     8041 agatggtaat ttggtaaacg aagagatgtt ttctaattac aattatgaga ttaaagataa
     8101 agaattgctt aatacagtca agtttaaata tattaataaa gaaaaaatac agttaaaagt
     8161 aagtgaaaaa aattatgtaa tggagattgt accttatcaa attttgacaa ataagttata
     8221 tgaatcttta ccatgtgcag atggatattt tgttccaaat aaagagtatt caaaactttg
     8281 tgttgttgag agacatagaa tgactggaaa tgtagcaatt gctccactaa aaggatttgg
     8341 cataaaaaat ggagcaattg ctactactgt ggcccatgat tctcataata ttatagttgc
     8401 aggggataat gatgatgata ttctagtagc tataaattac ttaaaggaaa tacaaggtgg
     8461 ttatgtaatt gtatcaaatg gaaaagttct tgcacaccta agtcttcaag tagctggatt
     8521 aattagtact tttacagctg aagaagtaca agaaattact gataatatgt tggagatagc
     8581 aagaaaaatg ggagttccag agtatgtaga cccttttatt acactttctt ttatggcact
     8641 tccagttgtt ccacaaataa gacttacaga tttaggtcta tttgatgttg aggaatttaa
     8701 atttatatag ataatataaa agtaataata aatgaggctt tctcaaaata gggataagtc
     8761 tctttttatt gttgaaatga tggaaatata tttgatttca tacaaaaaag agcatctcat
     8821 gaactaaaaa ttttattttt agatatcctt attttaatta atctatcatt taaccttgtt
     8881 ttaagcatag atatcttaat aaaactattt tcagtctatt ttaacatata aatacatgaa
     8941 aattatatat ttatgaaact ttatacataa aaaataaaat attttattta tatatagatt
     9001 tatagggtaa taatataagc atagtataaa attattaata aaaacaaaag gagagatatt
     9061 tatgttgagt ataggaacaa aagcaccaga atttacacta gaagataaag atggaaataa
     9121 ggtaagtatg tcagatttta aaggcaaaaa agtagtagta tatttttatc ctaaagataa
     9181 tacaccagga tgtacacgtc aagcttgtgc ttttagaaat gcatatgatg gttttaaaaa
     9241 ggaagatata caagttattg ggataagtaa agatagtata aaatcacatc aaaaatttgc
     9301 agaaaagcat gaacttcctt ttattttact ttctgaccct gacttagttg ctataaaagc
     9361 ttttgatgta tggaaagaga aaaaaatgta tggaaaaact gcattgggag ttgtacgtgc
     9421 gacatatatt atagatgaaa atggaattat tgaaaaagtc tttgaaaaag caaaaccaga
     9481 cacaaatgca caagagattc ttgaatattt ggaaaaacag gagtaataag aatttatgat
     9541 tataatgtta tcaccagcta aaaatatgaa gaatatagaa gtatttgata gagatttgag
     9601 tttgccttgt tttatagata acacaaaaga aatagtggaa aatataaaga catttgctat
     9661 agaagatttt aaaaataaaa tgaaaattaa tgaaaaatta gctgtattaa ataaaaatag
     9721 atttgaaagc attaaatttg atagattagg aaaccctgca atcttaactt atgatggaat
     9781 acaatataaa aatatagaag cagaaaattt tacaaggaaa gatgaagagt ttgcaaatag
     9841 ctgtattcga atcataagtg gattgtatgg tgttgtaaaa ccatatgata gtatatatga
     9901 atatagatta gaaatgcaga caaaattacg agtaggtgag tttaagaatt tatacgaata
     9961 ttggggaaat cgtatatata aagaactaat caaagaaaaa acagctatcg ttaatctttc
    10021 atcaaatgaa tacagcaaat ccatagaaaa atttattaaa gatagtgata catatattac
    10081 atgtactttt aaagtaaata aaaatggcat attgaaagta gaatctacac aagcaaagaa
    10141 agctagaggt atgatgacaa aatacattgt aaaaaatagg ataagagata ttgaagaact
    10201 gaaaaagttt aatttggaag gttataagta taaagaaaac ttatctaaca atagtgaata
    10261 catatttgta aaagagtaga agttttgtta aatattaaat ggcattgtta aatttaggta
    10321 aaatatactt agtgtgtatt gaattgaact tgattttaaa atataattat tcttaaatta
    10381 aaaaaagaaa ggagtggtct tccaaaatgg aaaatcacaa acagatgcct tggcattcaa
    10441 gagcaacgca tgaaatttta gaaagtctag attcatcaga ggaggggctc accgataccg
    10501 aggcggaaaa acggcttaaa gagaatggtt taaatgaact tagaaaaaaa ccacctagaa
    10561 caactttaca aatgctttgg agtcaaatta cagatgctat ggttatgata ctaataggag
    10621 cagcaatatt atctttgata tttggcgaat ttacagaagc ttttgtaata ctaataattg
    10681 taacagtaaa tgcagttatt ggaattgttc aagagaaaaa agcagaatca tcattagaag
    10741 cattaaaaaa tatgaactca cctacagctc gtgttatgcg tgatggtgaa gaaaatgtta
    10801 ttccagcaag taatcttgta gcaggagata ttgttttttt agaagatgga gctatggttc
    10861 cagcagacct tcgtttaata gaaacatcaa gtttaaaaat tcaagaggct tctttaacag
    10921 gagaatctgt tccatcagaa aaagatgcag atacaatctt acctaaagaa tgtgtattag
    10981 gagaccgtag caatatggcg tatacatctt caatagtcac atatggtaga ggtgtaggtg
    11041 ttgtagtagc aactggtatg gatacagaag tgggtaacat agcacatctt ttagattcac
    11101 aagatgattt tgatactcct ataaaacgta aacttaatac tgttggaaag acattaagtg
    11161 tcataggtat tattgtatgc gttgttatat ttgcaatagg tgcattttat cagagacctc
    11221 tcttgccaat gtttatgatt gcaatttccc ttgccatatc aattattcca gagggattac
    11281 ctgcaacagc tactattgta atggctttag gcgtacagcg tatggctaaa agaaatgctt
    11341 taattagaaa attacctgct gttgaaacat taggaggagc tacagtaata tgttgtgata
    11401 aaactggtac gttaacctta aataaaatga ctgttacaca tattgctgtc aatggagatt
    11461 ttgaatctgg aaaagtgaca tcaatagata ctgcatctga aaaacatcca ttagtgtata
    11521 aagagattgt ttatgctggt gcactttgta atgatgcatg tattaatcca gataaaacag
    11581 atgaaatatt aggtgaccca acagagggcg cattaatttt tatggccaaa aaatttggaa
    11641 tagatcaaga agctcttgaa gaagaatatc cacgtgtatt tgaacagcct tttgattcag
    11701 atagaaaatg tatgacaact gttcataata tagatgaaaa gattattgct tatactaaag
    11761 gagcagtaga cgaaattctt gacctatgta ctaaaatatt aacttcaaaa ggtgaaagaa
    11821 acattactga agtggataaa aagaatatac acgaattatg tcttaacatg tctaaagatg
    11881 cattgcgtgt acttggtttt gctaagcgtg agatttcatc tataccaaaa gaagatagtg
    11941 aaaatataga atatgattta acatttatag gtatggttgg tatgattgac ccaccaagaa
    12001 ctgaggtaat tgatgcagta agtacttgta aagaagcagg tataagaacc ataatgataa
    12061 caggtgacca taaagttact gccacaacaa tcgctcatga gttaggaata tggagtgaag
    12121 aaaatactgt gatatctggt gatgaacttg ataatttgag tgatgatgaa ctagatgaag
    12181 ctgttaaaaa tactacagta tttgctcgtg tatccccatt tgataaactt agaattatac
    12241 aatctctaaa aagaattgga gaagtaccag caatgactgg ggatggtgta aatgactctc
    12301 ctgccttaaa gtctgctgat attggtatag ccatgggtat aagtggtaca gatgttgcaa
    12361 aagattcttc tgacatgatt ttaatggacg atagttttac aacaattgct tatgcaataa
    12421 aagaaggtag acgtgtatat agaaacatac aaaaagttat acaatttttg cttgttggta
    12481 atatagcaga gatacttacg ttgtttgtag caatattatt aaattgggat actccgcttc
    12541 ttgctgttca tattctatgg gtaaatcttg caactgctac gcttccagcc cttgctcttg
    12601 gagttgaccc accaagcaga aatataatga agcaccagcc agtgaaatca accactttgt
    12661 ttgaaaaaaa tcttatacaa agagttgtta cccaaggtat atttgtggca ataatgacta
    12721 ttttagcata taaaattggt cttaatatag aaaatcattc tgtaggtcaa acaatggcat
    12781 tttgtgtatt agcattttca cagatgttac gagcatttag tcagcgttca aatacagatt
    12841 caatgtttaa tagaaataat ggtaaaaatc catttctttt aatctcattt ttaacttctg
    12901 catttttaat agcagtaata ctatttgtac ctgtttttag aaatgctttt aacttaacag
    12961 ttttaaataa cttagagtgg cttataacga ttgttttagc cataatgtct gtaattcaag
    13021 tagaggttgg gaaaattatt aaaagaaaaa aattaaaaaa atagttgaca aaattaggaa
    13081 aaatatataa gatataaaac atagaaaaat taaataaatc ttatccagag aggtggaggg
    13141 actggcccta tgaaacccgg caaccagtat atttatatac attggtgcta aatcctgcgg
    13201 cgaaagctga gagatgagta taattaagct tatacagagc ctgagttata tactcaggct
    13261 ttatttattt taactctcat ctggtaaata gtttaatttt ttttactaaa atctaagggg
    13321 gatacaagaa tgtataataa agaaacaata tgtgtgcaag gaaattataa accaggtaat
    13381 ggagaaccaa gagtactacc tttatatcaa agtacaactt ttaaatatag cagtatagac
    13441 caacttgctg aattatttga tttaaaagtt gatggacata tatattcaag aataagcaat
    13501 cctactattc aagcttttga agaaaaaata agtttactag agggtggagt atcttctgta
    13561 gctgtatcat cagggcaatc tgcaaatatg ttggcagttt taaatatatg taaatcagga
    13621 gatagtatac tttgttcttc aaaagtatat ggaggaacat tcaatttact aggacctagt
    13681 cttaaaaaat ttggtataga tttaatatcg tttgacttag attcaagtga agatgagata
    13741 gtagaacttg caaaggaaaa tactaaggtt gtgtttgcag aaacacttgc aaatccaact
    13801 cttgaagtca tagattttga aaaaatagca aatgtagcta agagaattaa tgttccattt
    13861 attgttgata attcattagc atctccagtg ctttgtaacc ctttaaagta tggagcaaat
    13921 atagttactc attctaccac aaaatattta gatgggcatg cttcaagtgt tggaggaatt
    13981 atagtggatg gtggaaactt taactgggat aatggaaaat ttccagaatt agttgagcca
    14041 gacccaacat atcatggtat aagctatact caaaaatttg gaaatgccgc atatgcaact
    14101 aaagcaagag ttcagttgct tagagactat ggaaattgtt taagcccatt caatgcgtat
    14161 cttactaatt taaatgttga aacactacat cttagaatgg agagacatag tgaaaatgca
    14221 cttaaaatag ctagattttt agaaaaacat gaaaatgtag attggattaa ttacccagga
    14281 cttgaagata acaagtatta tgagaatgcc aaaaagtatt tatcaagagg atgtagtggt
    14341 gttttatcat ttggagtaag aggtgggtta gaaaatgcca aaaaatttgt ggaaaaatta
    14401 cagatagcat ctttggttac acatgtttca gatgtaagaa cttgtgttat acatccagct
    14461 tcaactactc atagacaatt aacagaagaa caattaattg catctggagt attgccttca
    14521 ctaataagat tatctgttgg aatagaaaat gtagaggatt taatagctga tttaaatcaa
    14581 gctttaaatt tctaatattc taagagaaag agaagtgaaa agatatggct ttaatactac
    14641 caaagggatt acctgttatt aataaattat tagatgaagg aatagatgta atttataaag
    14701 aagattttaa aaaggaatta aagtatgaag aaaacataga cactaagata gctatactaa
    14761 atctaatgcc tataaagata gatacagaat tagatttact aagaaggata gataaaacag
    14821 gattcaacgt gtctgtggaa tttattaaaa tttcaacaag agaaagtaaa agaagttgta
    14881 atgaatatgt taaagaattt tataaaacat ttgatgaagc caaaggagag tattttgatg
    14941 gctttataat aacaggagct ccagttgaac aaatggagtt tgaagaagtt gattattgga
    15001 atgaactaga agaaattatg gattattcta agagaaaaac taaatctaca ttatatatat
    15061 gttgggcagc tcaagcaagt ctttacaaat actacaatgt aaaaaaactt ccactaagtc
    15121 aaaaatgttt tggagtattt aagcataagg tagataaaga tagtaaaata gtagatggat
    15181 ttgaaaatga attttttgct ccacattcaa gacatactac tgtaaatatt gaagctttga
    15241 aagagaataa agagttgagc attgtgagtc attctaaaga agctggtccc tatataatta
    15301 caaattcaag ggatgtgttc gtaatgggtc atagcgagta tgacaaatac actcttgata
    15361 aagaatataa gagggatata aataaaggag ataaaatatc tatacctcaa aattactata
    15421 ttaatgatga cccttcagaa gagccaactg taaaatggaa aaaacattca gaattgttgt
    15481 ttagaaattg gataaaaaac tatttgatac aataaatttt aagagtatat aaatgttaac
    15541 taaaaaaata tagaaattta taggtataaa gttaatctta atgatacttt ataccttatt
    15601 tttttaaaat ttgaccttat aatagtagtt ttatatttta agaaaatcac tttaggtgta
    15661 tgtagataga ggtggcttta attttatata tggtgcgata atgggagact aaacctttag
    15721 agtatcaata aatttgtatt ttaactttta aaagagatta aaaagggaga aatcagtgta
    15781 aaatatatat atcgattttt tattaaattt tattaaaaat atcctgtata aaattaaata
    15841 tcaataagat aacaaaattc caattgaagt tacaatattg taattgacaa taaatactta
    15901 atactataat atttaataca ttaaacatta tagtgttaag tatttattta cagtatgcac
    15961 ttcagcctaa atttgaaata aagtattttc gtagggagcg tttgtatgga tgagatgagt
    16021 tttaagaaag agctactaga gttgactaga gatataaata tgaaatttac aactttatta
    16081 agtgattttt atcagccact tggtattaca gcagtgcaag ctttgatttt atctgagtta
    16141 tgtgaacatg gagagaaaaa aataagcgat ttagggaaga acttaaacat gacaaatagt
    16201 aatgtatcag taatctgtca aaggcttgaa aaaaatggct ttttaaatcg cataagagat
    16261 atagaagacc aacgaattgt aaaagtaaaa gttacaaata aatcattgga tatacaagaa
    16321 catatttcaa gtagtatttt tgatagttat tttgaaaata tgaccagtga aaaattgcaa
    16381 gatatggaag atataataga aggtttagag aaattaaata aacttttaac ttacattgat
    16441 tgcaaataac ataaaaataa tttaaaggag aataaaatta tggatgaaca taaaccccaa
    16501 aaaaggtcag cactctatta ttatacagtt ataataatag tagtaatgtt tttaaatgct
    16561 ttaatatttc caagtttaat ggataaaaaa atggttgaga ttgactatgg aaagtttctc
    16621 acaatgttgg atacaggaaa ggttaaatct gttgagatag aaaataatag aatagcaata
    16681 aatccatcta gtagcaagga taagaatata tacataactg gtagaatgga tgacccagaa
    16741 ttggtagata gactaaaaaa tgcaaaagta gaatttacta aggttattcc taaggaaaat
    16801 tcacctcttt taagtatttt acttacatgg ataataccta taggtattct tatgctattt
    16861 ggtaacttaa tgatgaagtc tatgcaaaag cgaatgggtg gtaatgctat gcaatttgga
    16921 aaaagcaacg ccaaaatgta tgtatctgct caaagtggaa aaagatttaa tgatgtagct
    16981 ggtcaagatg aggcaaaaga agctttaact gaaatagtag atttcttaca caatccagaa
    17041 aagtataaaa agataggagc acaaatgcct aaaggagcac ttttagtagg acctccaggt
    17101 acaggaaaaa cactccttgc aaaagctgta gcaggtgaag caaatgtacc atttttctca
    17161 atatctggtt ctgagtttgt tgagatgttt gtaggaatgg gagcttctag gattcgtgat
    17221 ttatttaaac aagcaaaaga aaaagctcca tgtatagtgt ttatagatga gattgatact
    17281 attggtaaaa agcgtgataa tggaagtgga ttaggtggaa atgatgaacg tgaacaaact
    17341 ttaaatcagt tgttaagtga gatggatggt tttgatggtg gaattggagt agtaatactt
    17401 gctgctacaa atagacctga cagtcttgac aaagcattac ttcgtccagg aagatttgat
    17461 agacgtatac cagttgaact tccagacctt ggaggacgtg aagctatatt aaaggtacat
    17521 gctcaaaatg taaatgtaag ctcagatata gattataatg caattgcaag gtctacatca
    17581 ggagcatcag gtgcagaact tgcaaatatt gtaaacgaag cagcactttt agcagttaag
    17641 tctggcagag atatagtaga acaacaggat tttgatgaat ctgttgaaac tgtaattgct
    17701 gggtatcaac gtaaaggagc tgtaatttca gataaggaaa agaaaattat agcatatcat
    17761 gaaataggtc atgcacttgt tgctgctatg caaaaacatt ctgcacctgt ccataagatt
    17821 actataatac ctagaacttc aggagcattg ggatatacta tgcaagttgc tgaagatgag
    17881 agtgtactta tgtctaaaga agaagcaatt gataaaataa ctacttttac aggtggtcgt
    17941 gctgctgaag aggttatctt taacacttgt acatcaggag catcaaatga tatagaacaa
    18001 gctactaaaa ttgcaagagc tatggttact cgccttggta tgagtgaaga gtttgatatg
    18061 gtcgctcttg aaactgtaag caatcaatat ttaggaggag atacttctct tgcttgttct
    18121 tccgacactg ctgctaaaat agatgaagag gtatttaaaa tagtaaaggc ttgtcacaaa
    18181 aatgcaattg aaatcttaga aaataatatt gataaattgc atgaacttgc taattattta
    18241 ttagaaaaag aaactattac aggtgatgag tttatggaaa tacttaactt aaactaaaat
    18301 gattatacac aagataaaaa tgcctattgt taggcatttt tattttacaa ctaaattttt
    18361 taaattcata tttaaatctt tacataatct taatcattaa aaatacatct taactacaat
    18421 tttatatcgg aattgatatt ataatagtaa tgaatggtgt aaggaggttt tttaatggat
    18481 agacctaatc ctgatattct attagaaaaa attaaaaatg aagaagaaaa actaagtaga
    18541 ggtcaattaa aaatattctt tggttatgca gctggagtag gaaaaaccta ctctatgttg
    18601 gaatctgcac aaaatttaaa aaaagtgggt gtagatgtag tagttggata tattgaacca
    18661 cacactagac cagaaacatt ggctttactt gatggcttgg aaattcttcc tgtaaaggag
    18721 atagaatata aatctataaa actaaaagaa tttgatttag atgctgcttt agcaagaaaa
    18781 ccagaagtta tattagtaga tgaatttgcc cattctaatg ttgctgggtt aaggcataca
    18841 aaaagatggc aagatataga agaattgctg ttagcaggca taaatgtcta cactactgta
    18901 aatgtacaac atttagaaag tctaaatgac attgttgaga taattacaaa cgtatcagtt
    18961 aaagagacta taccagataa gtttttggat acaaatacac agttagaact tatagatgtt
    19021 gaacctgatg tacttcttga aaggttcaat gaaggtaaga tatataagaa agaacaagct
    19081 actagagcaa aaaataattt ttttataaaa gataacctgg ttgcacttag agaaatcgca
    19141 cttagaaaaa ctgcagagag agttaataaa gaagttcaaa tgaccagact atcaaagggt
    19201 gatgtaactg taatacctac atcagataca cttcttgcat gtataagtcc atctccatct
    19261 tcagcaaaag ttattagaac tgcatccaga atatcagaca gtacatttgc aaaatggata
    19321 gctttatatg ttgaaactcc aaacacagca agacttccat ttgatgaaca aaaacaacta
    19381 caaaataacc taaaattagc taagaaatta ggtggagaaa taattgtttt acatggtgaa
    19441 aatataatag agcaaatact tagaattgca aagctgagaa atgtaacaaa aattgtaata
    19501 ggtagaaatc attcaagtaa taaaagattt agtaaaagat ttaaaaaaga tatagttgac
    19561 aaattaattg atgaagttga ttatatagat atacatataa ttccatataa atttgcctca
    19621 gatgttaaat acaaacctaa aaaggataaa tcatcaataa aatctaaatt taaaattagc
    19681 aaagttgatt ttataaaact tatatttatt actttgatga taagtatttt agcatatgca
    19741 gtacaaagca tgggatttat aagagaaaat atattgttaa tatatatgtt gggtgtagtt
    19801 ttagtttcta tgtggactaa agggtatagt acaggtataa taagttctgt atttaatata
    19861 gttcttttaa attatttttt tacagcacca ttatatacat tatctatagc agattcaaac
    19921 tatatagtaa cactggttgt attctcaata gtaggtataa taactagcac tcttacgtct
    19981 aagatacagc atgaagcaga aactgctgct aaaagagaag aaaatacaaa aatgatatat
    20041 caaataatta agggattttt aagattatca aataaagatg atatagtaaa caaaggtata
    20101 gaacttttgt ctcttagctt aagtagagat gtaatttgtt atttaaatgt agataagcat
    20161 aataaatcaa agctttacaa aaaaaatact aataataagg ataaaaatga tttggatagt
    20221 gaagatgaaa aagctgttgc aagttgggtt ttatccaatt caacagtggc aggaaatgac
    20281 acggatacac ttcctggttc aaagggatac tatataccaa ttgtaggaat gaatttaact
    20341 ttaggagtag tgggaatatc atgtattgat tcaaaattgg atacagaaga tataagctta
    20401 attgaaacaa taattgctca aatggcaata gcgcttgata gagaaatctt gtcagaagct
    20461 aaagaaaata ccaatcttga gattgaaagg gaaagattaa gaagtaatct tttaagagca
    20521 gtttcgcatg accttagaac tccactagca ggaatatctg gtgcagtaag tacaattata
    20581 aaaaacaagg gtactatagg tcaagatata atagatgaat tacttagtgg tgtatatgaa
    20641 gatactcagt ggttaataag acttgttgaa aatttactta gtatgaccaa gatagatgaa
    20701 ggaaaattgg aagttaaaaa acattctgaa ctggttgagg aaattatatc agaagcgtta
    20761 caaaaaataa aaaaaagaat tgaaaatgca cttatagata ttaatatacc agaacagata
    20821 ttatttgttc ctatggatgc aaaattaata gaacaagttt taattaattt aatagataat
    20881 tctttgaaat attcaaagga agattgtaag atagagataa atgtatatga gaaagatgat
    20941 tatgtttggt ttgaagtatc tgataatggt cctggtatat caaaggaact taaaaagcat
    21001 atctttgaca gattttttac tggagaagaa ggagctaaag attccagaaa gggtgttgga
    21061 cttggattat caatttgtaa atctataatt caagctcata aaggtgaaat tatggtagaa
    21121 aacaataaag ataagggttc aacttttaag tttagtttgc ctaaggaaaa tgaatagttt
    21181 taaattttaa aattagtttt ggaggaagaa atgagtaaaa aacttatatt gttagtagag
    21241 gatgataaga caataagaaa atttatatct actgcacttt taacacagga ttatgatgtc
    21301 aaggaggcta taactggaaa agaaggtata tctattgctg tctcatattc tccagatgtt
    21361 gttcttttag atttaggctt agaagatatg gatggaatag aggtaataaa agctatcaga
    21421 caattttcaa atataccaat aattgtagtt tcagctagag agcaagatag agacaaggta
    21481 gaagtatttg atgcaggggc agatgattac ttaacaaaac cattctccat agtagagtta
    21541 ttagcaagag taagagtagc atttagacat tcacaagtag aagctcaaca aaaagatgat
    21601 gttaaatcta cgtttgaagt agataaatta ttgattgatt ttgataaaag aaaagttatt
    21661 gttgatgatg ttgaagtcca tttgacacct attgaatata atattctttc attacttgca
    21721 aagcatcatg gaaaagtgct tactcataat tttataataa aagaaacatg gggtagtgtt
    21781 atagggaatg agactaagtc tttaagggtc tttatggcaa cgttaagacg aaaaatagaa
    21841 aaacaacctg caaacccaag atatataatt acagaagttg gtgtaggata cagattaaat
    21901 gatgaatagt tgtaatatat taattattag attatgtgct tattaatata tattaaaatt
    21961 atatagttta gttaaaataa gaatgaagtg tatgtgcttg ttgtcagttt agcacatgca
    22021 ttttattttt tagctttttt agtatcaata attttttagt atcaataata tgaaatatgt
    22081 tttaaaataa gtatatagtg taatctgtaa taaaaacttt ggttaaataa taaatgtatt
    22141 taacacttac cttataataa atttaatata aata
//
LOCUS       DAMJYC010000060        21974 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJYC010000060 DAMJYC010000000
VERSION     DAMJYC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.061"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            535..1842
                     /locus_tag="PC472_002510"
     CDS             535..1842
                     /locus_tag="PC472_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727827.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRRTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1928..2190
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2316..3119
                     /locus_tag="PC472_002511"
     CDS             2316..3119
                     /locus_tag="PC472_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2727828.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGETVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3157..3768
                     /locus_tag="PC472_002512"
     CDS             3157..3768
                     /locus_tag="PC472_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2727829.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3800..4471
                     /locus_tag="PC472_002513"
     CDS             3800..4471
                     /locus_tag="PC472_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2727830.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4770..5132
                     /locus_tag="PC472_002514"
     CDS             4770..5132
                     /locus_tag="PC472_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2727831.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5568..6269
                     /locus_tag="PC472_002515"
     CDS             5568..6269
                     /locus_tag="PC472_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2727832.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6540..7358
                     /gene="larE"
                     /locus_tag="PC472_002516"
     CDS             6540..7358
                     /gene="larE"
                     /locus_tag="PC472_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2727833.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7413..8561
                     /locus_tag="PC472_002517"
     CDS             7413..8561
                     /locus_tag="PC472_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2727834.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRILDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8697..10085
                     /locus_tag="PC472_002518"
     CDS             8697..10085
                     /locus_tag="PC472_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727835.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSIGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10114..10812
                     /locus_tag="PC472_002519"
     CDS             10114..10812
                     /locus_tag="PC472_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2727836.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10815..12248
                     /locus_tag="PC472_002520"
     CDS             10815..12248
                     /locus_tag="PC472_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2727837.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12270..13007
                     /gene="truA"
                     /locus_tag="PC472_002521"
     CDS             12270..13007
                     /gene="truA"
                     /locus_tag="PC472_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2727838.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13118..13325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13578..14777
                     /locus_tag="PC472_002522"
     CDS             13578..14777
                     /locus_tag="PC472_002522"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2727839.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15024..16070
                     /locus_tag="PC472_002523"
     CDS             15024..16070
                     /locus_tag="PC472_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2727840.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16178..17341
                     /locus_tag="PC472_002524"
     CDS             16178..17341
                     /locus_tag="PC472_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2727841.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSDYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(17430..17810)
                     /locus_tag="PC472_002525"
     CDS             complement(17430..17810)
                     /locus_tag="PC472_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2727842.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(17813..18472)
                     /locus_tag="PC472_002526"
     CDS             complement(17813..18472)
                     /locus_tag="PC472_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2727843.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(18578..19552)
                     /locus_tag="PC472_002527"
     CDS             complement(18578..19552)
                     /locus_tag="PC472_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2727844.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGILVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIAGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVKGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKTINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(19592..19939)
                     /locus_tag="PC472_002528"
     CDS             complement(19592..19939)
                     /locus_tag="PC472_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727845.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(20157..21113)
                     /locus_tag="PC472_002529"
     CDS             complement(20157..21113)
                     /locus_tag="PC472_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2727846.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            21556..21924
                     /locus_tag="PC472_002530"
     CDS             21556..21924
                     /locus_tag="PC472_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2727847.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         8713 a         2223 c         3638 g         7400 t
ORIGIN      
        1 ttatgaaaaa tttatataag taaaatatat taacataaag gtaaaatttt gtatactaaa
       61 agggaatatt tacagtttaa tggcagtttt aaaataataa attaaagctg tcatttttat
      121 ttaaaaattt ttagaaaaag tacaatagtg ttaatgtgtg aattagataa ggtgtatata
      181 atagtttaga tgatatgata taaaatatgt tgtaaattaa atgttttctt tgacaatata
      241 tacacaagga tgataatatc tatatgaata aaagatagtg tgtaatttga taatatgtaa
      301 ttataacatt atgatataat tacaataaag tatgtaataa tctgtagaaa tttcaagcta
      361 gttatctaag tcggataact aaatctaaac ggaatacata atgtattaaa taaatgatga
      421 tactaaaatg atactatttt tatagaaatt tattgaggta tataattaat gaaaataata
      481 tattaaatta tataatatat tgagttttac ataaaagcaa aggtgggaat aaaaatgaaa
      541 gaaattgctg atgctataac atcagagtat ttaagctatg gtatcaatga agatattagt
      601 atctatgaag gtagattttg catatattta gataaaaaat atagatgtaa tgggaaaatt
      661 tattataaaa tgacacctcc aatatctata agttttaaag cagacatagg ttgtgttgaa
      721 gagatagaca atgaagatga caatttagct ttagattatg ataatgctat tcttgaagtt
      781 catggatata aaattatttc tataacaata aatactttaa gtgaatttag tgtagaaggt
      841 tatatcaatg atgactgcat aaaatctaag aactcctatg ttgaatatgt agattttaat
      901 ataataaact tagataaaat acctggaaaa ctcattaaat acaatgataa ggtatacgca
      961 ggtagaattg aatttgatat aaatgattat gttgtaacta tagacaagcg atatgattat
     1021 agaaaagaat taaaatcaga attaaagtct aaatctggag caataataac tcatataggt
     1081 aggattagaa gaaaagatgg tagaattttt agaacaaata atacaataaa tcttttagat
     1141 aggatatcaa cagctttgag ttttatgtgt ggaagatatg tagggttttg tttggccaaa
     1201 gggtatagaa gtggtaatga agtttataga atatggaatg aaaatcaaat atctcctttt
     1261 agatatgtac caacatggtc agatacactt tcaaattatc ataatatgga aaaatatata
     1321 agccttatgt gtaaaaagct agaagatttt tattatggtt ctgctataaa gagtgttgtt
     1381 gattggtaca tagaatctct tggaagtgct accatggaaa acaatataat atctgtacaa
     1441 attgcacttg aaactttgtc atatgtaatt ctagtggaac aaaataagat acttacagat
     1501 gaagcatttg actgtaattt ggcatctaaa aatataagac tcttacttga tacatgtaag
     1561 ataccatatg gtaaacatga attaaatata tttgataata taataaaaaa taaatttgat
     1621 gatggagtgg atttagttat ttatttaaga aatagtatag ttcatccaag tagaaggact
     1681 catagagctg tgttagaggt tgaagatatt tggaatataa tttcaatagg aacgagatat
     1741 attgaacttg ttttactctt tattttaggt tatagaggag aatattcaaa cagattagta
     1801 gaaagatgtt atggtgaagt ggaagttgta ccttggaact agatttttat atttatttta
     1861 aaatatatat tgacatatat tgccttatgc tataaaatca atatcataat aataattcag
     1921 aataacatta aatgctaaga aaagaaatag taaaagtagt taatcttaaa cagagagttt
     1981 ccgattggtg agaggagcaa tagattttac tttgaacaca tctttgagca ggactctgaa
     2041 agagtagaaa tactttatta ggagatttcc ggaatccttg cacccgttat agtgctagag
     2101 tataataatt ttattgtact taaagaggtt agtgtcgtga ggcattaatg aacagaggtg
     2161 gaaccacgag agttaactcc cgtcctctag cagaagctag aggcgggagt tttttaatat
     2221 aattttttaa gcgcgcagag aattatattg caatagatac actttttaaa aaataatcaa
     2281 aatattttaa aaattaaaag acgagggaga atattatgaa aaatatatta aaaaaagtag
     2341 gaatatttac aattatgtta ggtttattag gtggagttgt aggttgttca aaaccagata
     2401 atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga agtagttgta gggtttgata
     2461 atacgtttgt accaatggga tttttagatg aaaagggaga aacagtagga tttgacgtag
     2521 acttagctaa agaaacattt aaaagacttg gaatggaagt taagtttcag cctatagatt
     2581 ggtcaatgaa ggaaactgaa ttaaatgatt caaaaactgt agatgtatta tggaatggat
     2641 attctataac agacgaaaga aagaaaatag tatcatatac agaaccatat ttacaaaata
     2701 agcagataat agttactcta agtgactcaa aaataaattc aaaagctgat ttaaaagata
     2761 aagaagttgg aacacaacaa ggttcaacag cacttgatgc agtggaaaag gataaagatt
     2821 ttatgaatag cttaaaaggt ggagcaccag ttctttatga tacttatgat aaagcactta
     2881 gagatttaga gataggtaga actagtgcag ttgttggtga tgaagtttta attagatact
     2941 atatgggaca aaagggtgaa gacaaataca aggtattaaa agacgatttt ggattagaag
     3001 attatgttgt agcaacttct aaagaaaatc cagaactttg tgagaaaata aatgaaacat
     3061 taaaagaaat gaaaaaagat ggaacttttg acaaaattta tgataagtgg tttaaataga
     3121 agataagtta gctgttaaga agggggcact aaaggcatgt tacaaggttt agagatagtt
     3181 atagcaatgt tttgtataac attgatagta tcaattccat tggggattgg agttgcattt
     3241 ttaagattat caaagaataa attagtaagt ggtattacac aatgttatat attgataatg
     3301 agaggtacac ctttactttt gcaaatgata gtcatatttt acggattacc tcttttagga
     3361 attgtatttg acagatttac agctggagta gtagctttct tcttaaatta tgcagcatat
     3421 tttgcagaaa tatttagagg tggaatacag tcaatcgata gaggacaata tgaagcttca
     3481 aaagtactag gatttgataa gtttactatg tataagagag taatctttcc acaagttttt
     3541 aaaagaatac tagcacctat ttcaaatgaa gtaatcacat tagtaaaaga tacatcttta
     3601 gtatatatat taggattaaa tgatatttta agaatatctc aaatagcaat gaacagggaa
     3661 gcaagtctgt taccattatt tgaggcagga gcgatatatc ttatatttgt agctatactt
     3721 actaagggat ttgaattact tgaaaaaaaa tattcttatt atagataaag ttacaatatt
     3781 ataggaggag gttttttata tgttgaaaat caaaaactta aataaatcat ttaaaaaaaa
     3841 tagagtactt aaagatattt cttttgagtt agaagaaggt cagattggag ttttacttgg
     3901 taagtctggt gcaggaaaga ccacaatatt aaggtgtata aatggtcttg aagaatttga
     3961 tagtggagag attataatag ataatgaggt aataaaaaat aagagagata tggcaaaaat
     4021 acgaggaaaa ataggtatgg tttttcaaaa tttcaactta tttcctcata tgacagtcct
     4081 tgaaaatata attgaatccc cagttaatgt ttttaaagtt cctagaaaag aggctgaaga
     4141 gagggctaga gaacttttaa gattagttga tttagaagat aaattaaatt cttatccatt
     4201 tgaattgtca ggaggacagc aacagagagt tgctatagct aggtcatgtg cattgatgcc
     4261 aaaagtcctt tgttttgatg aacctacttc tgcacttgat attgatacta ttcaaagagt
     4321 agtcaatata atgaatagac ttaaagacaa aggtatgaca atattaataa ttacacatga
     4381 tgttgtcttc tcaaataatg ttgcagataa aattataagt ataaaagatg gtatagtaga
     4441 aaatgttcaa ataaaagaaa aaattgttta gagttttata aatataaact agggtagaat
     4501 tttataatat aaataaaggg caagtaaaat caaatatact atgccctttt gttatctcaa
     4561 aatttaatct tatattttat gtttaagtta cattataatt tataataaaa ataaaaggac
     4621 tatttaaata tataatgtga ataaatgttt aataatttga aatatgataa ttttttatgt
     4681 tgaaagctta taatgattat gatataaatt ggttatagta tacaatttat gggtataaca
     4741 ataaaaaatt agaaaggaat ttttataaaa tgattaaatt atatggatat acaaaatgct
     4801 ctactgttaa aaaagctaaa aattggctta aggaaaataa tttagaattt gaagatatag
     4861 atatggttca aaaccctcct agtaaagagg agttaaaatc aatttataag actagtggat
     4921 atgatataaa gaaatttttt aatacaagtg gtatgaaata tagagaattg ggtcttaaag
     4981 atattgtaaa aactgaatct gatgataaac ttttagaaat attggtaagt gatggtatgc
     5041 ttattaaaag accattactt ttggatggta aaaatgtatt attgggcttt aaagaagatg
     5101 tatggaaaag tactttgcta aaagaagatt aggaagagat aggtgtgttg attaaaatta
     5161 ttaattaata cacctataaa tttatgcatt aaatctaatt tttataaata ttaattagca
     5221 aattcaaatc aaaaaaattc atatcaacaa acatattact tcttattaca aattatatca
     5281 tatgttttat attaaaatat taaaatatta caacgaattg ttacaaaatt acttattatt
     5341 aaccatgaac atttgttttg tgctatcata tttatataag ggaattgtca taaaaaatat
     5401 cttgttcata cagttttata tagaaaaaag aaatgaatct tttctttata taagtatatt
     5461 ttgtaaattg cctctatatt tatagtttaa ataatagtgg tattaaaaat ctgacaacaa
     5521 aaataaaact ttcaaaaaat taaatctaaa aagaatagga gataaatatg agtattgaag
     5581 atttatatga ttttgaatgt gttcctttga acaatgagat tgatattgat gttttatttg
     5641 aaaagatgat agaagatgga aaagaaaaag aatatacacc aattatagtc attgatgaaa
     5701 aagtagggct attaagaaaa aatatagata tgattactaa agagtatggt tctcttgaaa
     5761 actatagaga gtattgttta acaaaatata atgaaattga tgtaaatcaa ttttttgatt
     5821 ttaggaagag tgagatttca gaattattaa aggagctaat tgatttagaa gatgattttt
     5881 atgattataa acattttaga ccaattatga atatagatat atttcatgaa tataacaaag
     5941 tttacatagc taaaattcca acaattaaac catatgaagt atttgcatac atacctattg
     6001 gcggatttaa tgattgtcct agagatgaag aacatatagc tattgcaaaa tattggtatg
     6061 aaaagcatgg aatctttcca atcgcaattg gatgtgatac agttcaatat tctgtaaaaa
     6121 atttaactaa agatggtaaa aaatttgatg acttatgtat tgaattagta ttttattgtg
     6181 aggatattat tatacaaggg tatgaaacct taaaggctct taaggatgta ttaaaacgct
     6241 ctacaatagt gcttttttgg tgggattgat attctatcat ttagatagta ttaaattgtt
     6301 agtcaccaag aatccacttg ctttagttat ggggagtgtg tcaaactagg tttttataca
     6361 cagtgtagca tagtaatatg tttaaattac aaaggttaaa aaaatacttt aatattttta
     6421 acattataat taatagattt aaaggtaatt ataatttata aaataaatac tttatatgtt
     6481 ataatcatta agaatgtaaa aaaataatat atagttttat agttcatatt tttccagata
     6541 tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa attaaaaaaa atgttacttg
     6601 aattaggcag tgttgtagtt gcttattctg gtggagtaga tagtaatttt ttgcttaagg
     6661 tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac tattcatgca atgatgcatt
     6721 caagtaggga aattgaagag gctaaacaat atactcaaaa ttttggtgtt aaacatatca
     6781 tattaaatat agaaaacttt gatttaaaag aatttaaaga aaatggtgtt gatagatgtt
     6841 atcattgtaa aaaatatata ttctctaaaa taaaagaggt agcaaaagaa cataatataa
     6901 agtacatagt agatggaaca aatatagatg atttaggaga ctatagacct gggcttaagg
     6961 cattaagtga attaggagta attagtccat taaaagatag tagtttaaaa aaagaagaaa
     7021 ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa taaaccatca tttgcatgtc
     7081 ttgcgagtag gataccttat ggagttgaaa taacagatga aaatttaaga atagtagaaa
     7141 agagtgaaga atatctatcc aatttaggtt tttctcaatt tagagtgaga atgcatggag
     7201 atatagctag aatagaagtt ggacaggaag aactaggtaa attttttgaa aataacaatt
     7261 ttaataaagt tgatactaaa ttaaaaatat ttggctttaa atatgttaca ctagatatgt
     7321 caggatataa aatgggaagt atgaatttaa atgtgtagat tattttaaaa ataaataagt
     7381 tttatagtta atataaggaa acaggaggtt ttatgaaaaa ttatacgtta atatcgccat
     7441 gtttttttgg aatggaaaaa atgctggcta gagagattac taatttagga tatgaaataa
     7501 taaaaacaga agatggaaga ataacatata aaactgatga gtttggaata gcaaagtcaa
     7561 acatgtggtt gagatgtgct gaaagagtac atcttaaaat tgctgaattt gaggcaaaaa
     7621 gctttgatga attatttgaa aatacaaaaa gaataaactg gtctagatat ataccttatg
     7681 gagctcaatt tcctatatca aaagcttcct ctataaaatc aaaattatac agtacaccag
     7741 atgtacaagc catagtaaaa aaggctatag tagaaagctt aaagaaaagt tatttggagg
     7801 atggtttgct taaagaagat aaggagaaat accctatttt tgtatttata cacaaagata
     7861 aagtaaccat ttctatagat acaacaggag atgctttgca taagagagga tatagagaaa
     7921 aggcaaataa agctccaata agagagactt tagctgctgg gcttatttat ttaactccct
     7981 ggaaagcagg tagagtttta gtagacccta tgtgtggttc tggtactata ttgattgaag
     8041 ctgctatgat tggtataaat atggcacctg gcttaaatag agagtttata tcagaaaagt
     8101 ggagaattct tgataagaaa atatggtggg atgtaagaaa agatgctttt aataagatag
     8161 ataatgaatc taaatttaag atttatggat atgatataga tgaagaatct atagatatag
     8221 ctagagaaaa tgctgaaata gcaggtgtag atgaatatat agagtttaat gtaggagatg
     8281 caactcaatt taaaagtgaa gatgagtttg gatttataat cactaatcct ccttacggag
     8341 aaagattaga agataaagac agtgttaaac agttgtataa ggagcttggt tatgcattta
     8401 gaaaattaaa aaattggtcg tattatttga taacttctta tgaagacttt gaatatgagt
     8461 ttgggcaaaa agcagacaaa aaaagaaaat tgtataatgg tatgttaaaa actaactttt
     8521 tccaatatcc gggtcctaag cctcctagaa ataataaata agttttacat gatttatttg
     8581 taaaatataa aattttatta aaataagtaa aacattatga tgccttgaaa cgtctaatta
     8641 ttgtgaggag atttttgtga acaaatttaa agatttatag gattaggagg agttaaatga
     8701 aagtgttaaa gcttattact gtagtagccc tgatgatgtt atttgttact ggatgtaata
     8761 taaaaactga atctccagaa caattagcga agatacctga ctatgatgat acaaaaaaag
     8821 tattgtatga tggtattgac cagttactaa aaccagattc atctatgata ttgccttcta
     8881 atacaaaaga agtaggaaaa ataaataagg tagatttaaa cagcgatgga attgatgaat
     8941 tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt gagtcaggta gggtttgtaa
     9001 ccttgagtta caatggagaa aaatacgtac ttggagacca ttttttagaa aatggcgaat
     9061 caatagaata tgctaatttt tatgatttgg actcagatgg atataaggaa gttatcttat
     9121 tggttaaatc caaagacaaa acgaatatgc atatatataa agtagtagat aatgaaataa
     9181 caaaaatata tgacttggat gcatcatggc ttcaaaataa agaagatttt aatgacatga
     9241 aagtaaaaat tggatatata gatggagatg acaagttaga catattgatg cttcatttga
     9301 ataataagac taatgaaatg tttgcaagtg ttgcaaattt tgatgggaaa atgaaaatca
     9361 aagattctgt taagtttgaa aatgtaaaaa acttaagtga attatatata acattaggta
     9421 atgttgcttc atctattgga aattcttctg cagctataaa aggtattgta cttgatattc
     9481 ctatattgaa agataataac tatattacgc agatacttta tatgaaagat gggaaaatta
     9541 acaaagcttt tagtgattat gataaaacta ttactaaatc ttattatata cctgtagatg
     9601 atattgacaa agataagatt atagaaatac ctatagttgc aggcagtaca ggtaataata
     9661 aaaatactta tagttctaaa tcatctgcaa ctataagttg gtatagatgg aatggaaaag
     9721 aaggtgctga ctcaagttta atatttacaa gtcagatata ttataattat aaatataatt
     9781 ttaaattatt tattccaaat aatttatttg ataaaatact tgttgaacag gaatttgttg
     9841 gagaaaaagc agtatttaaa ttttactatt ttgactattc agaccgtaag aatttattta
     9901 atatagtagt agagagtaaa aataaacttg aagatagaaa aaatacaggt actcaaaatt
     9961 ctattgtact tcaagagagc gatgaatata ctttcttact agttgtaaac aatgcaaatg
    10021 aaatggataa gctagatatg actatagacg cattaaaaga atatttctca ttaatatatg
    10081 gttaatatta tactcaattg gaggttttat tttatgaagg aaaagatact tatactagaa
    10141 gatgaaattg gtattagaag ttttgttagt ataaacttaa aaagggaagg ctatgaaata
    10201 gttgaggcag gtactggaag agaagctata gaaaaaatga caacagaaaa ggatattaca
    10261 atagctcttt tagatgtaat gcttccagat ataagtggta tagaagtatg taaatttata
    10321 agggagaatt ttgaccaagt aggtataata atgcttactg ctaaagctca agaagatgat
    10381 aaaatagaag gatttatctc aggagcagat gattacatca taaaaccatt tagtataaaa
    10441 gagcttttag ttagagtttc tgctcttctt agaagagtag caaaggatga ttcaagtgtt
    10501 aagtcgagtg agatagtttc tccaccattt atacttgata tagacaaaag aaaattattt
    10561 aaaaatggaa aggaaattga gcttacacca actgaatttt caatagtgaa atatctaata
    10621 tcaaatgcta agcagtcgtt aagtagagac cagatattag acgaagtatg gggaactaac
    10681 tatttatatg attttaaaat agtagatgta aatataagaa ggataagaaa taaaatagaa
    10741 gatgacccat caaaaccaaa atatatacaa actatttggg gttatggata ctgttttaga
    10801 aaggaagaat aatagtgtta cattttgaag gtctgagaaa aaaagtaata aaaaactatt
    10861 ttataataat tattataatg gttactctgt ttgaaggatt gttcatgttt tacatacaaa
    10921 actactatta tgattctgtc aaacagctac ttgaatctga aataaaatat gctgatgaat
    10981 ataatgctat tacaatggaa actacaagtt ttgagaaaaa agtaaaaaat atatttgata
    11041 aacaaccctt aactaaaaat tcagaatttg gaatttcaat aatagataaa gataaaaata
    11101 ttatattaga ccaatatggt tttaaaagta aagaaaaagc aaattatgaa gatgttaaca
    11161 atgcattaaa agatataaaa actaaaaatt taacacctta tacttataga ataccagata
    11221 caggggagca tgttatgagt atctccttac cacttaaggt aaataatata atagaaggtg
    11281 ttgttagata tacagtatca ttagatgcta ttgataatgc gatattaaag caagctacat
    11341 ggcttatatt agcaggaata tttatactta ttatagctat actaataagt cttaaatttg
    11401 ctgagactct tataaaacca ttacgagagc taaagaagtt tgcaaatgaa ttggctgtag
    11461 gaaattataa tataaaatta gaaaagatga agattgtaga tgatgaaatt ggtgatttag
    11521 cacagacatt tgaacacatg gcacatgaga ttgacaaaag tgaaaaatta aaagaggaat
    11581 ttatatcatc tgtatctcat gaactaagga ctcctcttac atctataaag gggtggagtg
    11641 aaaccttagg ttatgaaagt ataacaagag aagaactaga cttaggttta ggcattatac
    11701 aagatgaaac agaaagactt ataaaactag ttgaagaatt attagacttt tcaaggcttt
    11761 cttcagacag gataaaactt catgttgata tagttgatgt tgaaggttta attgtaggtg
    11821 ttgtaaatca acttaaagtt aaagcagctg aaaaagatat atcactttta tttgaatttg
    11881 aaaatgaatt tatagagaac attcaaggag ataaaaatag attaagacag gttctcataa
    11941 atttaataca aaattcattt aaatttacga gtcaaggtgg ttacataaaa gttgtagctt
    12001 ctcaagatga agagataaca accatatcag tagaggataa tggttcaggt atagaaaagc
    12061 aaaatttaaa taaagtgtta gataaatttt tccaagaaga ttataataag gctggtagtg
    12121 gattaggact agcaattagt aatgaaattg taaaacttca tggtggaaga atgaaaatag
    12181 aaagtgaaaa gaatgttgga acaaaaataa cttttaatat aaaaaataaa tttgctaaac
    12241 aagcataaat tcttagaaag aggattaaga tgagaaacat aaaaatgata gtagcatacg
    12301 atggttcaag atataaagga tatcaaaaat taggtgacaa taatatgact attcaagaaa
    12361 aacttgaaaa tgttttaagt aaaatgacta atgaaacagt ggagataata ggttctggaa
    12421 gaactgatat gggagctcat gcaagaggac aagtggtaaa ttttagaact aattgtatgg
    12481 attctttgga taaaattcaa aaatatttat atgaatattt accagaagat atagttgtaa
    12541 aaactgttga agaagttgat gaaagatttc attcaagata caatgtaaaa tcaaagactt
    12601 atatgtataa aattgacaat aataagtatc ataatccttt tataagaaaa tatgctactc
    12661 atgtaagtaa aaaattagat ttagatagaa tgagaaaagc aagtgagtat ttagtaggag
    12721 aacatgattt tactagtttt gcttcttcta aatctaagaa aaaatcaaat gtaagagaaa
    12781 tttattctat aaatataaaa gaagatgata atgttataga aatatatgta gaaggaaatg
    12841 gatttttgta taacatggta aggataatag taggtgcttt gatagatgtt ggtctaaaaa
    12901 gaagagctcc acaagatatt aagtatatgc ttgaatcaaa agatagatgt caatcatcag
    12961 atactgcacc agctaaagga ctatgtcttt ggaaagtaac atattagtca attatataaa
    13021 actctcttta aaataaaaat aaaataaatt atatagttga cataaatgga aaaataaact
    13081 attatatatt ttataagaga gatataaaac tttatattta aatcctgtga gaaagagagt
    13141 aacttctatg tgttttatag agagctgagg atggtggaag cttagcaaat aagtattagt
    13201 gaaaagaact ttggaggata ctaattgaat ttagtatgtt agtaggtaat ctcgccttaa
    13261 gagaaagagt ggataagttt atcttatcaa ttagagtggt aacgcggata tatttcgtct
    13321 cttggttttt ccaagaggcg ttttttaata tatttaatta ttagtgttta tctggtatgt
    13381 tgaacattta ctaattgatt agctaagttt atcaataaga gtggtaccgc ggatatttcg
    13441 cctcttagta ttttaaaata ctaaggggtt tttttagtat taaaatgatt ttatgtgagt
    13501 ctaaattctc agagtcattc aatattaaga atttctataa aataaaaatt ataatttaag
    13561 attgggagga attaaaaatg aaggaaaaag tagtattagc atattcagga ggacttgaca
    13621 catcaatcat aataccttgg ttaaaggaaa attatgaaga tatagatgtt atagctgttt
    13681 gtggaaatgt aggtcaggaa gataaaatgg aagatgtata tgaaaaagct ttacaaagtg
    13741 gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt tgtaactgaa actatattta
    13801 aagccgtaaa agcagaggct aaatatgaag gaaaatactt attaggaact tctttagcaa
    13861 gacctataat agctaaaaaa ttagttgaag ttgctcataa agaaggtgca aaatatatat
    13921 gccatggatg tacaggaaaa ggtaatgacc aagttagatt tgaagctaca attgcagctc
    13981 ttgacccgac tataaaagtt attgcacctt ggagaatttg ggatataaaa tcaagagaag
    14041 atgctataga ctatgctgaa aaacataata taaaggtaac agcaactaaa gctaaaatat
    14101 attcagtaga tgctaacctt tggcatgttt caacagaagg tggagatata gagcacctag
    14161 aaaatgaaca taaaaaagat gtttataaac aatgtgtaga cccagaagat gcatgtgatg
    14221 tagctgaata tgtagaagtt tactttgaaa aaggtgtacc taaaaaggta aatggcgaag
    14281 aattatctcc agttgcactg attcataagt taaatgagtt gggttgtaaa catggaattg
    14341 gtgttataga tatagttgag aatagacttg ttggtatgaa atcaagaggt atatatgaga
    14401 ctccaggagg aacaatcctt tatgaagcac ataatatttt agaaagtgct actttagaca
    14461 aagatacatt acatttcaaa caaatggtat cttataaata cggagaactt atttacaatg
    14521 gactatggta ttgtaaatta agagaatcta tagatgcatt tatggaacaa actcaagata
    14581 atgttacagg aacagtaaaa gttaaacttt acaaaggaaa tataaaacct gctggaatat
    14641 ttactgaaaa tgctctttat gatgaaggaa tatcttcttt tggaaatagt gaactttatg
    14701 accacaaaga tgcagaagga tttattaatt tattcacttt accattaaaa atcagagcta
    14761 tgaaagctgg aaaataataa aataaattat ttaagagtgt ataaaaacta tttttaggtt
    14821 tttatatgct ctttttttat ttgaatttat taaattatac agtaaatagt ataaaaaaat
    14881 atattatagt tccaaatggc ttaataatgt gaaaatataa gacctaaaat ataaatttta
    14941 taaatacaat agttattgac tctctcattg taagagggtt tatactgaat ctaatgaaaa
    15001 tttatagtga ggagatagat tatatgagaa aaataattga aattaaacat cattcacatg
    15061 attatgagtg tatgtggaat ggtatagaag atttatatat aaataaaacc ggtgaatgcc
    15121 ttccaaacga ttttttcttt ttactgtcag gttttggttc attttgttat atgaaaacaa
    15181 ataaagctga tttgaaaaga atggtagctt tgggagatgg aaggacaaaa cagatgtata
    15241 aatttttagc accaattgtt ggatttgaat acaaacatca tgagtttaaa aagtatgagc
    15301 aagcattaaa gaaagcaaaa tctgaaattg atgatggata tccagttatt ttaggagctt
    15361 tagatatgta ttatctacca tattataaga agctatatca taaagaacat attccttttc
    15421 attatatatt aatggtagga tatgatgatg aaggaatata tctgtatgat tgtggtagaa
    15481 cagaattatt acatctttct tatgataaat tacgtgaaag catgaattgc aattcccctg
    15541 gattaagcaa agaaaatact atttgcacaa taagaatgaa tacttcaaag aataaatata
    15601 acattgctag agaagctatt gctataaagc gtgatatgtt tttaaatcca cctaaaggat
    15661 ttttgggata taaaggtttt gaaaagttta ttaaagagtt accacagtgg aacactgaat
    15721 tggataaaga tgattatgat aagattttga ccaatatggt tatgtttttt ggaacagtac
    15781 caacaattcc aaatgcaatc aaaggaatta aagaaccaga tactgttcaa tttaaaggtg
    15841 gatttgataa aatgagtaga atgctcaaca gtattgggga ggaatacaaa aacgactctt
    15901 ttatacaagt agctgatatt tttgataaaa ctgctattat aattgagaaa atatccaata
    15961 ttataataga ttacttaact caaacatgtg atgatacaga acaacttccg ttattgttct
    16021 ctgaggtctt agagcatatg aaaacaggat atttaatctt ggatgtataa aaaatatatt
    16081 gttgacccta acattacgta atagtgtaat atgaaaatat agcttgaata tattagtagg
    16141 tattgatggc tgtaaaatta aaatttgagg tgtggatatg agaacagtga aacaagtttc
    16201 tgatttaact ggaataagtg tgagggcatt acattattat gatgaaatag gtcttttaaa
    16261 accaaataaa attacagatg caggatatag actttatgat gatgaatcta ttaaaactct
    16321 tcaacaaatt ttatttttta aggagattga tattccttta agagaggtta aggaaataat
    16381 gtcaagccaa tattttgata aggttgaagc tctaaaaaat caaaaaaaat tacttatttt
    16441 aaaacgaaag agactggatg aattgataga gcttataaat caaactctaa gaggagaagg
    16501 aaatattgat tttaaagagt ttgatatgag cgagtatttt gatgttttag aagaatttaa
    16561 aagagagcat aggaataagg taattaaaat ttatggtagt gtagaaaaat acaatgaata
    16621 tatagaaaga gttaagtcta atgaagagaa aattgctaaa atggctataa aacaatatgg
    16681 aactattgag aagtttgcta aagctataaa aacaaatttt agcagtgata tattgaattt
    16741 aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa gaaaaacatc caaaattaaa
    16801 agaattatat agaaagcttg tagaagattt aagtaggaat ccttcttcaa cagaccttca
    16861 agaaatagca aaagagataa cagatatatc taaaaaagat tatgaaattt tctctatgga
    16921 tactggtgat gacaactggt attatatggt tcaaaattat ctagtaaatc ctatgtggat
    16981 agaagaagta gataaaaagt atggaagtgg agcaggaaag tttattggac aagtactaaa
    17041 gacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac ttgtagaaga
    17101 tttgagtaga gattgttctt caagggaagt tcaaagcatt gttgaagaaa tagacaatga
    17161 aatgaaaaga agtaatgaat tttataaaat agacaatggt gaaagatact ttgattatat
    17221 gtcagagctt tatttacaag attctgacta tataaaagta actgataaga attatggtga
    17281 tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata ataattgttg
    17341 aaaatttgaa agtaaaaaat atgcaagttt tataaaagca caaaaataag gatgtagatt
    17401 agctccatct atatccttat ttaaagttat taaaatatat agtttacatc aaacttaaca
    17461 ctttcaataa tatttcccaa atatgttttt atactttcaa cttcttctaa agattttaat
    17521 ttattttcag tataaattgc tttgtccaga atattaaacc ttaatcctat ttcatcaaag
    17581 tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact cgtagatatt
    17641 gaatcagctt ctttttgagc tttttcccaa tcttcatttt ctatagattt ttctaaaaca
    17701 gatatattgt ctttatatcc ttctgtaaaa tcatatagtt tattgttaac ataaaatcca
    17761 aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa tattaatcac
    17821 cctttttatt ttgaataaat attttgtcat tttcatctaa aacacaaata agaacatctt
    17881 taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta tctttttgag
    17941 ctatgtctag atttttattt attattctac catccagtat aagtgggata ggtaaatgat
    18001 taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt agattaccat
    18061 cagtctccaa aatagcatat tgtacatctt ttaggttaaa gtatccttga atcctaagtt
    18121 gttctaataa ttcatctata ctaattcttt cttttttaag ctctttatat acaatttttc
    18181 ctttatctat taatacagaa ggtttacctg ataaaaatga acttaatttt ctacttttta
    18241 atgataagaa tgaaattaga gtctgcatga ttacaagtcc agatattgct gcaacaccat
    18301 ttatgattgg tatattatta ttttccattg ggacagatgc aacttctgca attaatagtg
    18361 ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg actctaagtg
    18421 caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc atatctatca
    18481 attactcctt taaaaaatat ttacacttag ttagtatatc ctatataaaa aatttaattc
    18541 aaagtataaa aaaccttaaa taattggatt caactattta aggttttttt gataatttta
    18601 atattaaatt ttttatggat tttctgttac ttttatttat tttaataaca gattcaacaa
    18661 aactcttttt atctcctaat tttaagataa aatcaggata ttcttcaaat tcattgagtt
    18721 ttattaattt gaaagaagaa gatagatttt taaagttaat agttttagag ttattcacta
    18781 atacacttct ttttttaaaa taaatttctt ttttacttat atttagtata gttaatattt
    18841 gtttaagata tattattata tcttctggag ttctatcaaa tttgagttca aaattttcat
    18901 cttcaaagta gggaatataa tataaaggtg ttttgagagc aggtaattta ttatataatt
    18961 cttcaagaat atcgatattt ttaggggagt taaagaaaaa gcatttacat acattcatta
    19021 aatccacagc atctgaaagt gagttatgtt taatatctcc ttttacagaa aaaatttttt
    19081 tcatattcag taaagatatt tgcttagata agattctatt attgtatgta atatgttgag
    19141 atataaaagg ttgaatgtca atcatcatat taaataactc taagcattta cctgtatatt
    19201 tatacactct aaatgaagtt agaaggccag ctatatcaaa atcaccccaa ttatatattg
    19261 taacatcatc aaatttacaa aaccatttat aaaaatcatc cataacatat aaaagattag
    19321 gagcattatt gatatctagt tggttcaatt tggttaaatt tttacattta tttgaaagta
    19381 ttttattgta ttttggctta actaaagagt aataattatc tattgtatta aattcgttat
    19441 caactaaaat tccaccaata gaaattaatt ccgcaccatt atattctttt ggtttatttt
    19501 tattatcatc tagtatatta aattcaaagt ctataaataa cttgtatttc ataaatctaa
    19561 agcacatcct caggtaaatt atttattgag cctaagcatc ataagaatca atagatataa
    19621 tgactctaaa catcaaaggt tcattacaac ttttatttat ttcataattt aattcaaata
    19681 gatcttttaa gatataattt ataagtattc tttcccaacc catttttaaa ttaactttag
    19741 agtctattaa tatattttct atctttttaa atatttctat atttgctggt attatactga
    19801 gctcaatatt acttttaaac gggttagact gtaaatctgt tataagtata ttttttaatt
    19861 cctctgtaag ttcagaaatt aaactatcac ctatgtattt ttttatgatt tcctctctaa
    19921 tttcttttaa atttagcaat attagtacct gctacttaca tagtagcttt tcctttcctt
    19981 ttaatactat tattcatttt tgaatcttat ttagatatat tttaagatta aaagtcttta
    20041 gctatataat tttttaccca tttttaactt atattaacat attacaggct tatatctcca
    20101 ccctgtttac attgaggatg gagtatattg cctattttga taaaaatgat aggttgttat
    20161 ttgcctattc ctttttctga taatttttct aacatatctt tggtcatttt atctaaatca
    20221 taactaaact taaagttcca ttcttttact gcactactac tgtctagaga gttaggccaa
    20281 gaatcagcta tactttgtct tacagggtct acatcatatt ctatcacaaa atcaggaata
    20341 tattttttta tagaatcagc tatttcttca ggagaaaagc tcatagcagt aatattaaaa
    20401 gcatttctat ggatgagttt atcagctgga gcttcgatta aatccactat tgattgaagt
    20461 gcatcaggca tatacatcat atccattttt gtaccttctg ctatgtaaga tttatatctc
    20521 ttatttttta atgcctcata atatatatca actgcataat ctgtagttcc tccaccagga
    20581 ggcgtaacat aagaaataag acctggaaat ctaacacctc tagtgtctac tccaaatttt
    20641 gagtgataat aatcacatag taattctcca gaaactttag tcacaccata catagtatta
    20701 ggtctttgta atgtatcttg aggagtcata ttttttggag aattttcacc aaaagcagca
    20761 attgaactag gtgtaaaaaa tttacaatcc aattctttgg ctatttcaag accatttatt
    20821 aatccattca tatttaagtt ccaagcttct agaggtttac tttcagcaac tgcagaaagt
    20881 aaagatgcta gatgaattat agtatctaca tcatattttt ttgcaatttc aaaaaaacga
    20941 ttcttatctg taacatctaa tatctcaaat ataccagatt cacatactgg attaccttct
    21001 tttactctcc ttgaactagc gattacattt tgttctccat actcatttct aagctttatt
    21061 gttaattctg aacctatttg tcctaatgca cctgttataa gtattttttt caattttaca
    21121 acctcctaag ggtttaattt atgtagtact acttattttt attttagcaa atcttttgtt
    21181 taaatacaaa gaattaaaga taataatata cgacttaatt gaatgaagtg cagtaagtgc
    21241 gtaaaaattt aaagctaata tataatggtc tattgtataa caataaaaga gtagattaaa
    21301 ttattatata tagattgtgt gactaagtca cagaaaataa agctaattta atatataata
    21361 aaagcatgtc aagtaaagct ttaaattaaa gtaactttat ataaaaagta atataaattg
    21421 ataaaattat aaatttattt atagtgaata taggttggat tttatagata caaaaatagt
    21481 atatatgata tgtagaccat tagcatgttt aaagtagcaa aacatataaa ataaaatttg
    21541 aaaggaagta taaaaatgac aagaccatca atatatcata gccaaggata cacttgtgca
    21601 gaggcattaa taaagtctta taatgaggaa cacaacacag atataccaat ctcaatagga
    21661 agtggtatgg gggttggaat gaatgttgga agtgtttgtg gagctgtaaa tgctgctgtt
    21721 gttataatag ggtatttaaa tgggcgaaat agtaatttag atgaaaatat agcaaggaag
    21781 tattcaagag agctattgaa tagagtaaga aataaataca gctcagaaat atgtgtatct
    21841 cttaaaaaaa ataaagtaac ttgtgaagaa ataataaatt tcacatatga tgctctaaat
    21901 gatatgctaa aaaatcaaaa ataattctaa gaaacaagta ttttacattc gatagaatac
    21961 ttgttttttt atga
//
LOCUS       DAMJYC010000061        21942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJYC010000061 DAMJYC010000000
VERSION     DAMJYC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.062"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC472_002531"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC472_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2727848.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC472_002532"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC472_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2727849.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC472_002533"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC472_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2727850.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC472_002534"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC472_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2727851.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC472_002535"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC472_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2727852.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC472_002536"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC472_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2727853.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC472_002537"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC472_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2727854.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC472_002538"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC472_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2727855.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC472_002539"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC472_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2727856.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC472_002540"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC472_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2727857.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC472_002541"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC472_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2727858.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC472_002542"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC472_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2727859.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC472_002543"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC472_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2727860.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC472_002544"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC472_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2727861.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC472_002545"
     CDS             6989..7174
                     /locus_tag="PC472_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2727862.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC472_002546"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC472_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2727863.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC472_002547"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC472_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2727864.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC472_002548"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC472_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2727865.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC472_002549"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC472_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2727866.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC472_002550"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC472_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2727867.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC472_002551"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC472_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2727868.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC472_002552"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC472_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2727869.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSDEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC472_002553"
     CDS             11117..11767
                     /locus_tag="PC472_002553"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2727870.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC472_002554"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC472_002554"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2727871.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC472_002555"
     CDS             12536..12805
                     /locus_tag="PC472_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2727872.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC472_002556"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC472_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2727873.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC472_002557"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC472_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2727874.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC472_002558"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC472_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2727875.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC472_002559"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC472_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2727876.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC472_002560"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC472_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2727877.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC472_002561"
     CDS             14901..15848
                     /locus_tag="PC472_002561"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2727878.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC472_002562"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC472_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2727879.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC472_002563"
     CDS             16330..17163
                     /locus_tag="PC472_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2727880.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC472_002564"
     CDS             17151..18017
                     /locus_tag="PC472_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2727881.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC472_002565"
     CDS             18011..18814
                     /locus_tag="PC472_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2727882.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC472_002566"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC472_002566"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2727883.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC472_002567"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC472_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2727884.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC472_002568"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC472_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2727885.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="PC472_002569"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="PC472_002569"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2727886.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8818 a         2561 c         4493 g         6070 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata aaaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaatgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tgacgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tttagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga tgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatac aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aaatttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa tagggacaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJYC010000062        21916 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJYC010000062 DAMJYC010000000
VERSION     DAMJYC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.063"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            32..1144
                     /locus_tag="PC472_002570"
     CDS             32..1144
                     /locus_tag="PC472_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2727887.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSISKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGVLLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWIIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC472_002571"
     CDS             1398..3422
                     /locus_tag="PC472_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2727888.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKLIDQISKIAKNGTSKNRVSGADRHETNANVIKN
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC472_002572"
     CDS             3642..4328
                     /locus_tag="PC472_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2727889.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC472_002573"
     CDS             4423..5478
                     /locus_tag="PC472_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2727890.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAISKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFRSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="PC472_002574"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="PC472_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2727891.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGNGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="PC472_002575"
     CDS             7195..8901
                     /locus_tag="PC472_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2727892.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSIIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDECGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="PC472_002576"
     CDS             8923..9996
                     /locus_tag="PC472_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2727893.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="PC472_002577"
     CDS             10008..10730
                     /locus_tag="PC472_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2727894.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="PC472_002578"
     CDS             10746..11903
                     /locus_tag="PC472_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2727895.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="PC472_002579"
     CDS             11906..12745
                     /locus_tag="PC472_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727896.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="PC472_002580"
     CDS             12760..13935
                     /locus_tag="PC472_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2727897.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="PC472_002581"
     CDS             13964..14713
                     /locus_tag="PC472_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727898.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="PC472_002582"
     CDS             14735..15643
                     /locus_tag="PC472_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727899.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="PC472_002583"
     CDS             15691..16443
                     /locus_tag="PC472_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2727900.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="PC472_002584"
     CDS             16397..17764
                     /locus_tag="PC472_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2727901.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="PC472_002585"
     CDS             17770..18861
                     /locus_tag="PC472_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2727902.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="PC472_002586"
     CDS             18945..20858
                     /locus_tag="PC472_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2727903.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="PC472_002587"
     CDS             21057..21764
                     /locus_tag="PC472_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2727904.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
BASE COUNT         8743 a         2289 c         4051 g         6833 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaagataaa
      241 aaataataaa tccacactga gtataatact tagtatatcc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attatctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agcttagtat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact ttcttatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaag acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtgtat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tgggagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttgtatttt ctttattgta
     1081 tgtactattc tggattatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagac gctaaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgctgtctg
     1441 tggcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aatcacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaggatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaacttata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag aatttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccacaataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggatctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag aaatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc aatatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaattt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaggagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaaaata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagaatta gtcaacctag tattacctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaaccac tttgggtaat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt attccaaaga
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atccaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagattcta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaaac aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ttattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaatg tgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagtaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa taatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaatg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcgacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtact atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcagca tgttttttaa
    11101 tggtagctat attattattt aatctatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccaa
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaatt
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgagaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agctgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggctca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagagcttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagcgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttatgg caactttagc ttatttaatt cctatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggtgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcatatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg tttttt
//
LOCUS       DAMJYC010000063        21851 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJYC010000063 DAMJYC010000000
VERSION     DAMJYC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.064"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(61..1221)
                     /locus_tag="PC472_002588"
     CDS             complement(61..1221)
                     /locus_tag="PC472_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2727905.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(1392..1595)
                     /locus_tag="PC472_002589"
     CDS             complement(1392..1595)
                     /locus_tag="PC472_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2727906.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(1682..2716)
                     /locus_tag="PC472_002590"
     CDS             complement(1682..2716)
                     /locus_tag="PC472_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2727907.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEALKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3238..4599)
                     /gene="rlmD"
                     /locus_tag="PC472_002591"
     CDS             complement(3238..4599)
                     /gene="rlmD"
                     /locus_tag="PC472_002591"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2727908.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVKA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(4626..5348)
                     /locus_tag="PC472_002592"
     CDS             complement(4626..5348)
                     /locus_tag="PC472_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2727909.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNTGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(5348..5677)
                     /locus_tag="PC472_002593"
     CDS             complement(5348..5677)
                     /locus_tag="PC472_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2727910.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6007..6933
                     /gene="fba"
                     /locus_tag="PC472_002594"
     CDS             6007..6933
                     /gene="fba"
                     /locus_tag="PC472_002594"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2727911.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7068..8699)
                     /locus_tag="PC472_002595"
     CDS             complement(7068..8699)
                     /locus_tag="PC472_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2727912.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9182..10219)
                     /locus_tag="PC472_002596"
     CDS             complement(9182..10219)
                     /locus_tag="PC472_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2727913.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10238..11023)
                     /locus_tag="PC472_002597"
     CDS             complement(10238..11023)
                     /locus_tag="PC472_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2727914.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11056..12189)
                     /locus_tag="PC472_002598"
     CDS             complement(11056..12189)
                     /locus_tag="PC472_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2727915.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12237..13364)
                     /gene="hadC"
                     /locus_tag="PC472_002599"
     CDS             complement(12237..13364)
                     /gene="hadC"
                     /locus_tag="PC472_002599"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2727916.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(13364..14590)
                     /gene="hadB"
                     /locus_tag="PC472_002600"
     CDS             complement(13364..14590)
                     /gene="hadB"
                     /locus_tag="PC472_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2727917.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(14605..15405)
                     /gene="hadI"
                     /locus_tag="PC472_002601"
     CDS             complement(14605..15405)
                     /gene="hadI"
                     /locus_tag="PC472_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2727918.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(15444..16643)
                     /gene="hadA"
                     /locus_tag="PC472_002602"
     CDS             complement(15444..16643)
                     /gene="hadA"
                     /locus_tag="PC472_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2727919.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            17320..18318
                     /locus_tag="PC472_002603"
     CDS             17320..18318
                     /locus_tag="PC472_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2727920.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            18680..19207
                     /locus_tag="PC472_002604"
     CDS             18680..19207
                     /locus_tag="PC472_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2727921.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19234..19830
                     /locus_tag="PC472_002605"
     CDS             19234..19830
                     /locus_tag="PC472_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2727922.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(19909..21492)
                     /gene="asnB"
                     /locus_tag="PC472_002606"
     CDS             complement(19909..21492)
                     /gene="asnB"
                     /locus_tag="PC472_002606"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2727923.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         6766 a         3992 c         2611 g         8482 t
ORIGIN      
        1 cttcaataaa aaagacaatc ttaattaatt gtctttttta atatatattt ggctaataca
       61 ttattttaca caaacattac aagggtctat tttaccttgt gacttagctt gttttaatgt
      121 tactttattt attgttttac ttctttttag tgtagaacaa ttttttgtta gatgatatga
      181 ttttccatta ggagttacat atactgtact tttatcagtt aatccaggat tattagagct
      241 aggctcatta cttggtttgt tcgtattatt tgaattagta tcttttacaa tacctgtatt
      301 tgaatcccca acattattag ttatatttaa acattgtttt ataacaacat catcaatatt
      361 tccaatagca gttattttac ttgcattttt taatatactc ttattacttc catttccaac
      421 taacactata ggtgtttttt gtgcaattgt agaaccaact aaagaataaa ctaaatcagt
      481 tccacttgct atgtaaaatt cttttgagcc attataaaat ttatttatta tttgtttatt
      541 tgtatcgtat ctatctactc cacctattct tgttgcttta gtttcattta ttaacttatc
      601 actcatactg acttttccac caattgcata agtttctaat ccatctgtag caaaacttgt
      661 agtatttcca tcagttaata ctattggaga tttatctcta actgctatag aagcaacact
      721 catagcatct ggttcgcctt taaaagcatt agttaatatt acttttttaa ccgtacttac
      781 tcttcctatt tcttttgcaa ctttatgact tgtatctatt ctatcagttc cacttattct
      841 atttatgctt atttttttat cacttaatat tttctgaact gatgctccta tcgaattttt
      901 tcctccaatt agatatactt cttttacctt ttctaatctt tttaatgttt ctgaagggat
      961 tgtatctttc ttagttaaca atattggggc atttttagct cctgacaatc cacttgctga
     1021 caatccatca gataatccat tttcagaatt aataagtatt gcaatatcat aattttgttt
     1081 atctgctatt aaacctgctg tttcatactt atttgtacct tttattgaat taatagaact
     1141 aagagcgttt gatgttggac aactagcaat caataatgaa acagacatcc ctattgccaa
     1201 tagtattttg cttaatttca taatgaaatc ctcctataaa ttttataata ttacttaaat
     1261 attataattc tacataaaag aatattttcc tttatgaaat tagtttatat ttcaaaattt
     1321 tgtcactttt taaatctata tactaaaaaa tagactattt gtaacagtct atttcttagt
     1381 atatcaaata tttatttagg tttacgtttc tttgtactag tttttgtata tgatattcca
     1441 gttccaggta tactcaatgt agctgatttt cttccttttg aattaactga cactctagca
     1501 cctttaacac cagcacttat acctacactc ttcttaccta aatttaattt gagtcctcct
     1561 cctagattta tgcttttcct aaagttaaat cccatagata taaccttccc ttctatattt
     1621 taaatattta tacttaaaat tatatttgtt atacacttgt catcattggt atatctagca
     1681 tctatatctt tttacgtttt ttcttcttat atactactcc agcaactaca gctgctgttc
     1741 cagctccaac cccataagta gcatttctac ttttctcttc ttctcttttt tctaaacctt
     1801 catcatatac accttttaac ttagtttcaa tagacttcat ttcatctggt atactaaaat
     1861 cttcttcatt gtaaccttga ttatatacac tttcttttgc tttaggtaac ttaaccttac
     1921 cattatcata tccttcttta aaccaattag catatttaac attacctata gttgatgtgt
     1981 caaatctttc tcctttgata gcactctcaa atccttgagt agtgtaatca ttttttaatt
     2041 gttcttgtat ttcgtcataa gcattattat aagcatctat aaattcagaa tctatgtttt
     2101 cttcaaatgt cattttttct ttatctttat tgctatcctc tatcccttta gctttatatt
     2161 cttctatctt tttagtctta tattcattgt atccagttga atatccggta tcataagcat
     2221 tttttaaagc ttctttttca tatgtattat tagatttagc acctgtaagc cctaaattat
     2281 atccagtctc tttagccacc ttttcttcct cttctaattt agccttacct ttttcatatc
     2341 cttctgagta ctttgattca tatgtatcac tataatctcc actataattt gatgaactat
     2401 aatttccttt atacccatct tcataccctt gattatatcc tttttctcca acagacctct
     2461 tttgcttttc ttcattatta accgaagttg ttgttccaga actgcttcct cctccagtat
     2521 aaggacaacc accattatta tgtaaatgag ctggataacc accacaatga tagtgataac
     2581 ttcctaatcc agatttattt ttattatcac gatgacctcc tgatgaatca gttcttcctg
     2641 aatgagcata cgaaaagctc cctacactaa tcatagttat actcattgca acagctatta
     2701 actttttctt aaacataatc tatttccccc taaaatattc tatgatttta ttatatcagt
     2761 caatatatta tgtttaaacc gttaaaattc gacaatgtaa atatttgatg aattgtattt
     2821 gtaaataaat cttttagcat atcttgaata tctgtagttg ttttgacatc atattcttca
     2881 ataagttttt taatatttcg tttttgttaa acataaaaag cgtgcacttt cctttgtata
     2941 gtgatatttg atattttaat tatcactact ttagaagaat acacacttta ttttaaaaac
     3001 cctgatacta tgttattaga aaatggtact aatataaaag atatccagca tatactcgga
     3061 catgctaaaa cttcaacaac tatggataca tatttacatg taactaataa catgagaaac
     3121 cagacggtaa atatttttga aatcattgta gaataaattt gccacccaaa aataattatg
     3181 gtggcaaata ggtggcaaat ctacttttaa gacacactat aaaagtctga aaccctgtta
     3241 ttctctaata agtttaacta cagtctcaca atgtggcgta tttgggaaca tatccataca
     3301 cttaaccatc ttaatctcat aaccataccc cttaaaatca accaaatcaa caaccaaaga
     3361 cttaggatta caagaaatat aaacaatctc cttagcacca aatccacaaa tatccctaat
     3421 agcatcctta tgaatgcctg gtctaggagg gtcaacaata atcaaatcag gcttgtcctt
     3481 taaatcctta acaaccttag ccacatcccc agcaataaac tcacaattag taagtccatt
     3541 caacttagca ttctcattag cagcaacaac agcttcctca actatctcaa taccatacac
     3601 tttcttagca gcaccagcca taacctgtcc aatagaccca gtaccactat acaagtcaaa
     3661 tacaacttta tcattgtaat ctccaataaa atccctagca atactataaa gaacctcagc
     3721 tcccttagta ttggtttgga aaaacgaaaa tggtgaaatc ttaaacttaa gacctaaaat
     3781 ttcttcttgt atataatctc ttccatacaa tactcttaac tcatcacatt ggactgcatc
     3841 tgctaaacca tcattaatag tatgaagtac acctactaac tcagctttta aatcaatccc
     3901 taaaagcata tctttaaact catacatatc aaaatcttct tgagaagaag ttactatatt
     3961 aaccataatc tcattagtat gaatcccttt tctaactaca agatgtctca aatatccttt
     4021 gtgattcatt gttctataat aaggaagctt tttttcattg aaaaactcta cactagaaac
     4081 taatatcttt gagaagtctt catcaaccaa catacactcc tcaacagttt gtatatctat
     4141 atgctttcct tttttatgaa gacctaatgt caaaggtcca ttttttactt catctccaaa
     4201 agtatattcc atcttatttc tataatattt gttttctgga cttccttcta tccctaaaaa
     4261 ctgaaatcca aataaatctt gctttaaaaa taaatccata acttgttttt cttttatttc
     4321 taattgtttt tcatatggca cagacaatag tgtacatcct ccacactctc taaagtgctt
     4381 gcaagtttct tcagtttcaa gtggtgagtt ctctaataat tctatcatct taacttcagc
     4441 tttcttactt ctaacttttt taactccagc tttcaccttt tgaccactta ttccaccctt
     4501 catatggata actctatctc caacttggct taaagatgtg ccaccaaatt ccattttatc
     4561 tacttcaaat tctataacat cttttttctt cacagacatt cctccaattt actatagtat
     4621 ctttattatc tttcttcaat caattttact tcttcatctg ttaactctct atactcacct
     4681 aactctaaac tttcatccaa ttttaaattg cccatagaca atctttttaa gtatactact
     4741 tttttgccta cgctttcaaa cattcttttt acttggtgaa acttaccttc atgtattgta
     4801 agctctattt ctgattcatc atcacttttt aatatattta actgtgatgg catagtttta
     4861 tatccatcat caagcactac accttctaaa aaagcttcta catcttcttc agtaactact
     4921 ccatcaattt ttgcataata agtcttaggc acatgcttct taggagataa aactctatga
     4981 gaaagctttc catcattagt taaaactaat aatccctctg tgtctttatc taatcttcca
     5041 acaggaaatg gttcaaaagc taagtatgat aaatcaatta aatctaatac tgttgggtca
     5101 tatttatctg tagttgctga tatatatcca tcaggtttat tcatcatcaa atatatatat
     5161 tctctatata taacttcttc tccattaaat aatatctcat cattttcagt gtctacttga
     5221 gttcctgtat ttgaaacttc acttccattt actactacag acccttgttt acaatacttc
     5281 tttatttcac ttctacttcc ataacctaaa tttgaaagaa ttttatctat acgttgcttt
     5341 ttagccatta aaaatgctct ccattctcat aatatttttc tttttcaaat actgtaaatg
     5401 ctaaatccat actttctaca tctaaattat ttcttatact atcagttatt attcttgcta
     5461 ctatatcttg tacttcttgt cctctatcaa accaagctac ttcaacaaaa ggatatacat
     5521 ctgctatttt accatctctt attgcaactg actttataca ttctatttca aagtagtctc
     5581 ttggacattt tacagcttca actaaatcat ttatcatttt ttcacttatt ttacatatat
     5641 cattttcatt aattcctctt atttttatct gtggcatagt ttatctcctt tattttaaat
     5701 ttttagtgaa ttatcatcca cttaattccc ttcgataacg tttgaagtgg taacttcttg
     5761 gtcaagacaa ctattgttat cagtatctcc aaactaataa ggtagttcct acccttatag
     5821 ttagcattat atagctaatt ttcctaaatt gtttatattc tttattattt tatttaatcc
     5881 tattgtcttc tatttaacat tatacttgta atttatagtt attacaattt actatatact
     5941 ttttttgatt taacgttata atataagtga tgggtaaatt ttaaaagtta ataggaggag
     6001 tttaaaatgg cattaattac tacaaaagaa atgtttaaaa aagcttacga aggtggattt
     6061 gctataggtg catttaacat aagtgactta gaacaattac aaggtgtttt aaaagctgct
     6121 aaagctaaaa attcttatgt tatgatacaa gcttctatgt cagctgttaa atatgctgga
     6181 cctcatacat tagttgaaat ggttaaggct gcttctgatg aaataggtgt agatgtagct
     6241 cttcacttag accatggtcc aaatatggat gctattaaaa cttgtattga tgctggtttc
     6301 tcttcagtta tgatagatgg ttctcatttt gactttgaag aaaatgttag aataactaaa
     6361 gaagctgttg aatatgcaca ctctaaaggt gttgttgttg aagcagaact tggtgttcta
     6421 gctggaacag aagatgatgt tacttctgat gttcacaagt atactcaacc agctgaggca
     6481 gttgagttcg ttgaaagaac aggtgttgat tcattagcaa tagctattgg aacttctcat
     6541 ggtgctttca aattcaaagg agaagctaaa ttaagattcg atatactaga agaaatccaa
     6601 agcaaattac caggtttccc aatagttctt catggtgcat ctgctgttga ccaaaacgct
     6661 gttgctacat gtaatgaatt tggtggaaat atagctggtg ctaaaggtgt acctgtagat
     6721 atgttaagaa aagcatcttc tatggctgtt tgtaaaataa acatggatac tgacttaaga
     6781 ctagctatga ctgctgctat cagaaaattc ttagctgaaa atccaaaaga atttgaccca
     6841 agaaaataca ttggtgctgg tagagatgct atacaagctg tagttgaaag caagatagat
     6901 gatgttttag gttctgctaa ctctataaac taatataact tttaaatatt tataaaagaa
     6961 aagagatgac ttaaagtaat taaatttatt tttaagccat ctcttttaag ttgtatcaat
     7021 tatgtatctt ataatatttt aattatattg tctttttaaa ctgacagcta aacgtgaata
     7081 tgtccactct ttatctttct atggatggta gaaggatata tacctataac ttcagcagcc
     7141 tttgttattg aaccatactt atttatagcc ttacttatca ttatttggtc cactatttta
     7201 tatgcatcat taagattcat aattccattt accactattg gtgataaatc atctgtttca
     7261 ttatcaatca tatctaaatt tatcagcatg ttaaattcat cttctccaat tacattttta
     7321 gctgataata ctacaaatct ttcaatgatg ttttttaatt ctcttatgtt tcctggccaa
     7381 gaatattctt ctaataactc aataacttta ggcgaaactt ctacttctct attgtacttt
     7441 tcattgtaaa gttttaagaa ataatttact aatggtacta tatcctcttt tctttctcta
     7501 agaggaggta tttttactgg aataacactt aatctatagt acaaatcttg tctaaattta
     7561 aggttatttc taaggtcttc atgagaaata ttagtagcac ttatatatct aacatctatt
     7621 tttataggct tactaccacc tattctagta atagtatttt cttgtataac cctaagtaat
     7681 tttttttgca tctgcattgg taactcacct atctcatcca agaaaatagt tccaccattc
     7741 gcctcttcaa aaagtccctt tttacctttt ttcttagctc cagtaaaaga accttcttca
     7801 tatccaaaaa actctgactc taataattca tttggtatag ctccacagtt tatagctata
     7861 aaaggtttgt ctctccttga actatactta tatattgttc ttgctattat ttcttttcct
     7921 acacctgatt ctccatttat aaatatagaa gaatctgagg ctgcagctct cttaacaact
     7981 ttttttatat tgtccataat cttactttga cttatatact ctgtgtcttc tgatatatca
     8041 ctatatttta tctgagattc aagcttaata acccttgaaa tatctctccc tgttagtact
     8101 cctcctgtaa gctttccctt agaatcccat cttggaactg ccgtatatgc aataagcttc
     8161 ccatctgggt aaacgatatt tctataaatc tttttcttca ctttaagcat gtttaatatt
     8221 attggttctt caactaatcc ttgtctcaca aggtctgtta tatttctacc aagtacctca
     8281 gttcttttaa aaggtaatat ctcatcactg acagtgttca tcttttcaat taccccatct
     8341 ttgttaaaca caacaacaac ttcatctata tgctctatta tatcttcaat tactttatca
     8401 gatattagag acttctcaaa taaataaact atattatttt cattattatt atctttcata
     8461 ttgtatatat gtgcagtcat ccttaactct ctgaaagcta ttttcttttt actatctgtc
     8521 agtatttctg ttttgtcaaa atttgaatct aattcattta tatttctagg cttttttata
     8581 tctagaaaac taaaaatact atctattaga tgattacagt atattatttc gcctgattct
     8641 ttacataaaa tggctggatt ttctacatgt tctataagcc ctattaaatt attatccatt
     8701 aatattcgca cctttcatta agtaatttat tgaactacat acaaaattta ttgtttgctt
     8761 acaataaata ccatcagcac atataatcaa ctccttataa tattcgtctt ttcaagtagt
     8821 ttatttcatt aaaacgtaac taagatataa ttttaatgat tatagtttgt taaaaatttc
     8881 attttgcttt taattgtatt ttatcacata tttagtaatt atttgtctat ttaatacaat
     8941 ttaattaaat ttaagattta tttaaaaaaa caaagataat ccttacagaa tataactcac
     9001 aatttgtaag caatatacat ataaggatta tccagattat cttgtattat aaaattttat
     9061 tcaaacagac tttttaataa ctgacaaata cacactagca ataacttatc aaagggttaa
     9121 taattatgtt actaactaat aagacttatt caatcagtaa tatttatata gtttgtatct
     9181 attaagcttt tatagcttct aataaatcat caacgctatc taatttttct ataagcattg
     9241 gaactatttc atgtaagtct ccaactactc catagtcagc gatttctaaa attggagctg
     9301 ctgggttttt gtttatagca attatgaaat ctgatgattg catacctgct aaatgttgta
     9361 ttgctcctga tataccacaa gctatatata ggtttggttt aacagtagtt cctgtttgac
     9421 ctacttggtg agaatggtct atccatccag catcaacagc cgcacgagaa gaaccaacta
     9481 ctccacctaa tttgtcagct aatttcttaa gcatttcaaa tccttctggt ccacctaatc
     9541 ctagaccacc tgatactata acatttgcat ctgtaagaga tactaaatct ttcttagttt
     9601 taactgtttc taaaacagta gttcttatat catcttgagt tattttgtag tctaatgcta
     9661 taacttcacc agttcttgtt tcgtcttttt cagctttatc cataacacca ggtctaactg
     9721 tagacatttg aggtctatgg tttggacaaa tgattgtagc cattatgttt ccaccaaatg
     9781 ctggacgagt ttgttttatt ttcttatctt ctgggtctat ttctaactta gtacagtcag
     9841 ctgttaatcc agttccaact cttgaagcta ttctaggtgc taagtctcta cctatatgag
     9901 ttgctccgaa aagcattatt tctggtttta tttcatctat tgcatctttt attacttttg
     9961 tatatgcatc agttgtatat ttttctaaaa gagcatcatc tgcaacataa actttgtcag
    10021 ctccaaaagt gattaattct ttagctaatc catcaacatc ttttcctaat aatattgcac
    10081 aaagttctgc atctacttct ttagctaatt ttcttccttc tcctaataat tctataacta
    10141 ctggagctat ttttccttct ctttgttctg caaatatcca tacgttttta taagaactta
    10201 aatcttttat atcattcata gtttgtactc ctccaactta tataatgtgc ttttgtttta
    10261 gaccgattat tattttacta gctatttcat cagctttacc ttctaagatt tctccttttc
    10321 ctcttggttc aggagtaaat gatctaaata ctttagttgg tgatgctttt aaaccaactt
    10381 cttctttatt tacatctaaa tcatcaattg tccaaacttt tacatcatgt ttatatgctt
    10441 ttactatttt atcaacagac atgtatctag gtgtatttaa ctcttttact gctgttaaaa
    10501 gtacaggtgt acttactttt attaattcgt atccatcttc taattgtctt tgaactatta
    10561 tatcttttcc ttctattttg aaatcttgta catatgtaac ttgaggaatg tctaatttct
    10621 ctgcaatttg tggtccaact tgtgcagtat ctccatctat tgcttgtctt cctgcaaata
    10681 ttatgtcata gtcacctact ttagatattc ccgctgcaat agtatttgat gtagcccaag
    10741 tatcagctcc tccaaatgct ctatcactta ataatattgc atcatcagca cccatagcta
    10801 aacactctct tagcataaag tctgcttgag gaggtcccat tgttataact gttatagtta
    10861 catcatcatg ctcatcttta atttttaaag cttcctctaa tgcatttgca tcatctgggt
    10921 ttaatatact tggaactcca tctcttataa gagtacctgt ttctttattt attcttactt
    10981 cgttagtatc tggaacttgt tttacgcaaa ctaatatttt caaagtctat acctccaatt
    11041 tcattagatt atttattatt ttaatacgtt tgatgaaatt accatttttt gaacttctga
    11101 tgttccttcg tatattgata cgattctagc atctctgtac attctttcga tttcataatc
    11161 ttttataaat ccatatccac cgtgaagttg taatgcttta taagcaactt catttgcaat
    11221 ttctgatgca tagtatttag ccatagcaga ttcttgagca actctcattc ctgcatctct
    11281 cttttgtgct gcatcataaa ctaatcctct agctgcacaa acttttgttt ccatgtcagc
    11341 tatagtaaat tgagtatttt ggaatttagc tataggctta ccaaattgaa ctctttgttt
    11401 tgtatattta acagcttcat ctaaagcacc ttgagctatt cctagagcta aggcagcaac
    11461 acctattcta ccaacttcta gagtacccat tgctatctta aatccttgac cttcttttcc
    11521 aagtaagttt tcttttggaa ctttaacgtt ttcaaatatt aagtcagaag tttcagttcc
    11581 tcttataccc atcttatctt catgagctcc tgttgagaaa ccatcccatt tactttctac
    11641 tataaacgct gatgttcctc taagtccttt acttctatct gtaactgcta ttactatagc
    11701 tacatcacag aatggtccat tagttatgaa agtttttcta ccatttaata tatagtggtc
    11761 tccaactaat tctgctgttg tttgttgtcc acctgcatca gaaccagctc ctggctcagt
    11821 taaaccaaat gctcctatta attcacctgt tgctatacct tttaagtatt tttgtttttg
    11881 ctcttcagta ccattgtaaa gtattggtaa tgaagcaaga gaagaaccag ctgtaacaaa
    11941 agtagctgtt gaagcacatc ttctagctat ttcttccatt actatagcgt aagaaacata
    12001 tcctgctccc ccaccaccat attcttctgg gatattactg cttataaatt ttgcatcagc
    12061 taattttttg attaattcct taggcattac acctgtttta tccatttctg ctggtaaagt
    12121 atctaattct ttactaacaa aatctcttac tgcttttctt aaaagttctt gttctttgtt
    12181 atataacata gcttcctcca attttcatat tttatagtaa atttatttta atgaacttac
    12241 gctaaactca tcatctcagc aaaagtttga atacgagttc tggcttgttc attattctga
    12301 gtttgttggt cgatttcaac atataaagta ggtattccac tatcttctat atcttttcta
    12361 actaaaggat aatcgtattc ttctgggtca cagaatttca tcatacagaa gataacacca
    12421 tctatatctt tctttttaac ttcatctact ataagtgacc cacgtttttt cttagggtca
    12481 taagctaatg aacatccttc tatgtttgac cattgtcttg ctaatctctc taacgcatca
    12541 tcacctgctg gtacatctgt tctgaattgt cttgtttctt gtgctaagtc gtcagctaca
    12601 actgatatat tgttgtcttc taaaatgtct aatatatctt tagaatcagc taatatacct
    12661 gttaataaaa ctttctttcc agaacagact tcttctggca tagcatttaa ttttgctatt
    12721 aaatctttca ctagctcagt atgttcttct tttctcataa agaacccact cttaattact
    12781 aagcttctta ttgatggttt tatagtatta gaatgtttat aagctacttc aacaaagtct
    12841 ctcatagttt ttctatgttc attgtaaact tctatacttt catgaatttt tgcttcttct
    12901 atttcatatc cacaaatttc ttcaagttct ctttttacgc ctttgtactc actgattaag
    12961 tattttacac cagcttcaag ttttctattt tgtgggtgta ctaatgatat atattttata
    13021 tgaggtactg ctgatttcca gttttgtcct aaacaaatta gtgtatcaca catacctggt
    13081 ataataactc cagataattc atcataagca ccttttaatc catattctaa acatgattgc
    13141 attattgaac atgcaaatgc agggaaatat tgtttagcta aatctaattc tgtttgtcct
    13201 ccccatatac caactggaag cattccagct gcatgtataa tttcttctgg gcaataaact
    13261 gggaaacaac ctatagcttt tttaccagtt tcacttttat atttttttac agccgcattt
    13321 ggattttcaa ctacttcttt cattttagat aaaatagctt ccattatatc tcacctctat
    13381 taagtttttt tctttcttcc attacttcaa ctaaaccttg aattcttgtt tcaaattgag
    13441 cattagtgaa agctctaggg tctgcttggt caccatcaaa tccagcatat ggtaatccag
    13501 tttcttcagc tgctcttctt tgcatttcat attgtattaa actcataagt ttacagcttc
    13561 tgttcatatg atagaatgct ccatcacatt taccctctac taaagaatca actctatatt
    13621 ttgtcatacg atctaggttt acattgttaa acatagtact atatgctact gccattccat
    13681 ctaaatcatt aacttcatat tgtaatgccc aagcatgtgg gtaaacacta cctgtcatgt
    13741 taactccaaa tttagctaat gttttcatct tgtatcctat atatggccaa caaggtatac
    13801 cttccatcat tattctgtat ttttcttccc ctctgaaaga agatttacca gttttcatgt
    13861 tgtcctctaa ttcttctata agtaacttaa atgcttctgt agtttctttt ttacctctag
    13921 cacaaactat tacagccatg taagtaaata agtcaaatcc attcattgga gaaggagaag
    13981 aatctgctgg taaactcata gaatacttcc atagtcttcc gttttcagct gatattttca
    14041 ttacttcttc aaacttctta gggtcaaatt tctttcctga tataatttct agttgtttta
    14101 tagcttcttc aaattgagct ttaatataat ctattcttga ttgagtaact tcgtcttcat
    14161 tattgaaagt tgtatcaatc attattaaag gtatatctaa ttctcttgaa atattttcat
    14221 accattttat aacttggtta catatattgt tacagcaaag taggaaatct ggagctggca
    14281 tatccaaagc ttcacatcca ccattttcta aaagaccaaa atttgttctt gcatatgcac
    14341 ataggtcaat agaatatcct ttagattcag ctatttcaca taattctaaa gaaccttttt
    14401 tagctgctac tccagctgct tggttttctg gatataatac gtttaagtca aatacttctg
    14461 ctaactcttg aggaaatact gatgttgacc aacctacagg tcttccttct tctttagctt
    14521 taaatgcatt tgcatattgt tcagctaata aatcattaat tactactcta gcttcttttt
    14581 tttcagacat tttcatccct ctttttatat atttttcact tctttttgtg attcttttgc
    14641 ttcatcaaaa gcatataacg ctgctcctaa agcaccagtt aattgaggta tatcaggtac
    14701 aataatttct gtgttgattt ctcttgccat agctcttaca ataccactat ttctagcaac
    14761 tccaccaacc ataactacat ttctttgtac tcctattctt tttactaggc tagaaactct
    14821 ctttgctact gaagtatgaa tacctgccac tatatcttca atttttgcat tttcagataa
    14881 atgtgatata acctcagact ctgcaaatac tgtacatgta ctgcttattg atacttcatt
    14941 ttgagaattc atagatatac ttccgagttc agatacatca acctctatta tttttgccat
    15001 tacatctaaa aatcttcctg tacctgcagc acatttgtca ttcataagaa agtttaatag
    15061 tcttccatta ttatctaatt ttaatacctt tgcatcttga ccacctatgt caataatggt
    15121 tcttgtctct ggaattatga aatttacccc tctagcatga cagcttaatt cacttatttg
    15181 cttatcagca tctgaatagt tcattcttcc atatcctgta actacaactt ttttaatatc
    15241 ttctcttgca agacctgtct tgccatataa tttttctaaa actcttgatg gtccagtagt
    15301 cccagtacca gaggatattg tttcagaagc tacaatatct tccccattct ttaagattac
    15361 tccctttgat gcagttgaac ctatatctaa tcccattgtg tacatcttta gcattctcct
    15421 tttaaaaaat ctagaattac taattaatat cttacaactt tactatcttt aaagttattt
    15481 attttctctt catcgtagcc taaagatttt aatacttcta cagtatgttg acctactttt
    15541 ggtgctggtg tatattcttt aattccttca tttctaaaca taactggagt attaactaag
    15601 acacctgtat ttccgctatc gtatgttttc ttaaataaga agtcatttgc ccaagcttgt
    15661 tcgtcatcta ataaatcttc acagctttga attttttcaa atggtaagtc tgcttcttct
    15721 aataaagctg accactcgtc taatgttttt tctaacatag cttctccaac tatcttaact
    15781 aaatcttcaa catgattaac cattgaatct atgttattat atctatcatc ttctaatata
    15841 tattctctat ttataacctt acagaatttg cctaaccact tgttgtattg tattaaagct
    15901 aattgaatcc atcttccatc tttacattta tatgtagtca ttaatgggct gtttggattt
    15961 tctcttgata aaggcatttc atttccgtat tgtgctgttg ttatcattgt tcccattcca
    16021 tatatagctg tatggaaaag acttactgtt actctctcac ctttaccagt ttgagctttt
    16081 ttatgtaatg ctgctaaact tcctgctgct agtgctagac ctgcatagtg gtcaccaaat
    16141 cctgctgctg tatttgctgg agatgttcct ttttccataa cagattggct aactcctcct
    16201 cttgcgaagt atgcagtata gtcaaatcct ggtttatctt ttaaaggtcc tttttcacca
    16261 tatcctaata tttgagagaa tattaatcct ggatacttat cttttatttg gtcataagct
    16321 atacccattt tttctaatgc ttgaactcta acattagtta caaatatgtc tgcttctgat
    16381 aataatttat gtaatatttc tactccttct tttgatttta cattaatact tacacccttt
    16441 ttatttccat tttctaattc aaacataggg ttttcatcat ctgatgctgg agatttaaat
    16501 gttccaccca taactcttat tccatcacct tctataggtt caatcttaat aacctctgca
    16561 ccccagtcac ctaacatttt tgcacaacat ggtgctgcga tgaaacttga aagttctact
    16621 actttaactc cttctaaaag cataacgttt tcctccttaa tagtatcaat tataaaattt
    16681 cgattaaatc actcataaaa tacttttttc tgatattaac tgtctgatgt caaagttcct
    16741 aatcttatct aaaataactt aaaaattatt aaaattattt tttattgttt tattgtctta
    16801 cacctatatt gttaagcatt tatcgtgcca actttatatt taatcaaaat atcgcttgat
    16861 ttgaatgttt tttaacaata tgcaattgaa taatcttttt catatcgtgg catcttgcca
    16921 ctatccattg cattttgcta ttctttctat gcttataaaa gtttttttta tatatttttt
    16981 tatgaaaaag cttgacattt caaaaaaagt tttcattgct aaactcaaaa acacatgtta
    17041 tcattgtaat taatcagcaa gttagttaac taatttcaaa cttagataat tttttaataa
    17101 tatttaatct aaaaaataac aatttctttg ataaatttta gagtaattat tacattaaat
    17161 aaaaaattac taaatatcaa aataacacag ggaaaacaat ttcaaaacta aatttttaac
    17221 cttaaaaatt ttgtataaaa aatataacct tatatgctac atattatttc taaatgaaaa
    17281 attaccataa taatttaata attacaggag ggtattgata tgaaaatact agtatttgga
    17341 gcacgcgatt atgaagaacc agtaataaaa aaatggtctg aagaacataa ggatgttcaa
    17401 gtggatattt atcctgaaaa catgactgaa gaaaatgtag ttaaagctaa agggtatgat
    17461 ggtatatcta tacaacaaac taactatata gataatcctt atatttatga aactttaaaa
    17521 gatgctgggg ttaaagttat agcttcaaga actgcagggg ttgacatgat acattttgat
    17581 ttagttaatg aaaatggact tatcgttaca aacgttcctt cttattcacc taatgcaata
    17641 gctgaattag ctgttactca agctatgaac cttttaagaa agactcctct agtaaagaaa
    17701 aaagtctgtg aaggtgatta ccgttggata gctgaacttc ttggaacaga agttagatct
    17761 attacagttg gtgttatagg tacaggaaaa ataggtgcta cttctgcaaa attattcaaa
    17821 ggcctaggag ctaatgtaat tgcatttgac caatatccaa atagtgattt aaatgatata
    17881 ttaacttaca aagattcttt agaagacctt ctaaaagaag ctgaccttat aacattacat
    17941 actcctttac ttgaaggaac aaaacatatg ataaataaag atactctagc tataatgaag
    18001 gatggagctt acatagtaaa tactggccgt ggtggtttaa ttaatacagg ggatttaata
    18061 gaagcactag agtcaggaaa aattagagct gctgcccttg atacatttga aactgaagga
    18121 ttgttcttaa acaaaaaaat gaatcctgga gaattaactg acccagaaat aaataaactt
    18181 ctttctatgg aacaagttat attcactcat caccttggtt tcttcactag tacagcgatt
    18241 gaaaatatag tttattctag tttaagcagt gctgtagaag ttataaaaac aggaactgct
    18301 actaatagag taaattagta ttttataaat atattttttc attaattagc tattatctaa
    18361 caaaatagcc tttaatttcc caaatataag agagtcagct cagtatatat tgagtagact
    18421 ctcttatatt attttttata ttaaacttgt actcttctta ttcataccaa attaaattta
    18481 tattaactta ttctaactac tcctactcta tacattttac ctatttttac ttttctaatt
    18541 atatacattt gaatgggtac aatctgtatt ttacaaaaaa aacacctaac aaactatgaa
    18601 aattgtatga ttaatattaa tatatttata agaaaacata acaattatgt tagctctgga
    18661 tttttaggga gggagtatat tggaaaaaat gaataaagtt acttctaaaa aaactaccta
    18721 tattgttact tcagcattat ttgcatccat aatatgcctg acaatagcat atatattaca
    18781 tattccagtt ggtggaaaca atggatatgt tcatatagga gatgctttta tttatctagc
    18841 tgcaactatt ttgccaacaa actatgctat agctgcttct gcaattggtg ctggtctagc
    18901 tgatttatct acaggtgctg ctatctgggt aataccaact ataatcataa aaccaatact
    18961 tgtattattt ttcacttcta aaagtgacaa gataataaat aagagaaata tagttgcttc
    19021 ggttgtagca ggtatagtag gattagtttt atacatgttt gctgaaggta taattattgg
    19081 aagttttaca agtgcatttg taatgagctt acttggctta ttacagccaa taggaagttt
    19141 tattgttttt ataatcttag gtatggcact tgataaactt gattttaaaa aacgttattt
    19201 taattaagaa tttaaattta tggagggaaa attatgaata tcttatatac aattgaaaat
    19261 agtatctatg taaatataac taatacatgt ccatgtagtt gtgttttttg tataagaaac
    19321 gaaaaagatg aagttgcaaa tagtggtagt ttatggctgg aacatgagcc aagtgtagat
    19381 gaagtaaaag aagccttcaa taaatacaat ttagacgact atgatgaaat cgtattttgt
    19441 ggatatggcg aaccattaat gagaataaat gaattgatag aggtagcaaa gtttataaaa
    19501 gaaaaaagta gcataaagat aagaataaat acaaatggtc taagtgattt aatacataac
    19561 aaaaaaacag ctattctgtt aaaaaatgtt atagatgcag tatcaataag tcttaatgcc
    19621 ccaaataaag aagcttataa tagagttact caaccaaagt ttggagaaaa atcatttgat
    19681 tatatgttag attttgcaaa agattgcaaa aaatatataa aagaagtagc tttttcagta
    19741 gtagatgaaa tatctccaga agaaatagaa gaatctaaac aactggcaaa aaaacttgat
    19801 ataccattaa gggttagaca taagaattag caagtaaaat tataaaacat taattataaa
    19861 acattaagaa tcacctgaat ggtctttcag gtgattcttc ctcttgtatt aacattttac
    19921 aaaatagatt tcataccaca ctaggaatgt gtaaatcatc caaatctttc tccaattatc
    19981 agagtttcca ctaatgtagt cattaaaaat ctcattaatc tcatctatgt taaagaattt
    20041 atctgctatc tcactattga atttatctcg gatgtcctta ttatattggt catcagccat
    20101 ccatatacga attggcacaa taaatcctaa tttatttcta aaagcaatct cttctggtaa
    20161 tgtttttgat gcagctgtac gaaaggcaat cttattctct tttcctttca ctttatattc
    20221 tgatggtatt ctggatgcaa tatcaaatat tcttgtatct gtaagaggca ttctgacttc
    20281 caatccaact gcagtactca ttttatctat attaaaataa atatctcctt ccaaccatat
    20341 ttgaatatct acatttgaca tgttagtaag tgtatctagt tcttctgttt cttcataaat
    20401 tccctttacc acttcaatag gaagcacatc aggattgtat ttcttaagaa tttttctttt
    20461 ctcaccctca ttcataatgt ttgtatttcc aatataaaat gtcttcaaat tctctccata
    20521 acactctgcc ttatggtata tattatatcc accaaaaaac tcatctgctc cttctccaga
    20581 gtaacagata tttgtgtttt cagctgctgc cttgcatcca atagcaaaaa cgatagcaga
    20641 tgcatctcca gttggttgct ccatatggta cattacataa ggtactgacg caaaatactc
    20701 ttcaggtgta attatatatc tcgaattttt acacccaaga atattagctg tctgttgtgc
    20761 taattttgat tcatcaaaac gctcttcctc ataaccacat gaacctgttt tttctatatc
    20821 tgacattgcc aatatataag atgaatcaac acctcctgat aggaataact ctgcagtttc
    20881 attctctgat tttacctctg gcataatttt ctgaagtgtt gtatgaattt catccgtcca
    20941 ttcctcaata gacttgctgt ggtcaggatg aaattcaggt ttccaataac gaccaataca
    21001 tactgtttta ttctgataga caaggtaatg tcctggcata agcttcttta ctccacggaa
    21061 gaatgtattt tcacctgcta catatgtgaa agacatataa atctgtaaca tgtcttcatt
    21121 taactctttc acaaatcctg gttgctccat aatcttacga atagatgaac catagagaag
    21181 cttcccattt tcagtctcat aataatagaa aggttttact ccaaactggt ctcttaggca
    21241 gaagagtttc ttttccacag tatcccataa tgcaaaagca aacattccat gcatgtgatt
    21301 tgccatatca tatccccatg tcttatatgc ttccactaaa attgctcgtt cacgctcatc
    21361 tctacataat gaagaatcga ttccaagttc ttcacagaga actttccagt ttcgaatatg
    21421 tccattgtat tctagaattt ttatcatata tgtgtctttt cgtgttaaat acttttcttc
    21481 taaatttatc atatgtgtat cctttcgtat taaaacattt aacttctaaa tttattaaat
    21541 gtttatatat atttattcac ttataatcac tgaaaaatcg acttttttca aagtaaattc
    21601 tttttgaatt agtactaatc tgctctttat taatggcatc aatatgatta tcaagtatta
    21661 tcagtgttat aacactgaat tttttattct tcataaatat tgctcccttt atattaccaa
    21721 ctaaatataa tatatcttat gttacttaca tattctttaa gaaattctga agattttata
    21781 aagttttttg tattataatc aattaaaaat aaaattctag ttataaagca tattttccat
    21841 taaacaaaaa a
//
LOCUS       DAMJYC010000064        21020 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJYC010000064 DAMJYC010000000
VERSION     DAMJYC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.065"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            217..1278
                     /locus_tag="PC472_002607"
     CDS             217..1278
                     /locus_tag="PC472_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2727924.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1291..1488
                     /locus_tag="PC472_002608"
     CDS             1291..1488
                     /locus_tag="PC472_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2727925.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1652..2356)
                     /locus_tag="PC472_002609"
     CDS             complement(1652..2356)
                     /locus_tag="PC472_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2727926.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2521..3246
                     /locus_tag="PC472_002610"
     CDS             2521..3246
                     /locus_tag="PC472_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2727927.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3306..4898
                     /gene="hcp"
                     /locus_tag="PC472_002611"
                     /gene_synonym="priS"
     CDS             3306..4898
                     /gene="hcp"
                     /locus_tag="PC472_002611"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2727928.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5270..6175
                     /locus_tag="PC472_002612"
     CDS             5270..6175
                     /locus_tag="PC472_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2727929.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKNVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6299..7252
                     /gene="msrA"
                     /locus_tag="PC472_002613"
     CDS             6299..7252
                     /gene="msrA"
                     /locus_tag="PC472_002613"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2727930.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNFHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7405..8535
                     /locus_tag="PC472_002614"
     CDS             7405..8535
                     /locus_tag="PC472_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2727931.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8652..9620)
                     /locus_tag="PC472_002615"
     CDS             complement(8652..9620)
                     /locus_tag="PC472_002615"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2727932.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            9955..10143
                     /locus_tag="PC472_002616"
     CDS             9955..10143
                     /locus_tag="PC472_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727933.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTCFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10403..11602
                     /locus_tag="PC472_002617"
     CDS             10403..11602
                     /locus_tag="PC472_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2727934.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11654..12157
                     /locus_tag="PC472_002618"
     CDS             11654..12157
                     /locus_tag="PC472_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2727935.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12264..12581
                     /locus_tag="PC472_002619"
     CDS             12264..12581
                     /locus_tag="PC472_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727936.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12739..13698
                     /locus_tag="PC472_002620"
     CDS             12739..13698
                     /locus_tag="PC472_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2727937.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13813..14598
                     /locus_tag="PC472_002621"
     CDS             13813..14598
                     /locus_tag="PC472_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727938.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14890..16209
                     /locus_tag="PC472_002622"
     CDS             14890..16209
                     /locus_tag="PC472_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2727939.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16284..16712)
                     /locus_tag="PC472_002623"
     CDS             complement(16284..16712)
                     /locus_tag="PC472_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727940.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16803..18179)
                     /gene="hydG"
                     /locus_tag="PC472_002624"
     CDS             complement(16803..18179)
                     /gene="hydG"
                     /locus_tag="PC472_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2727941.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18180..19265)
                     /gene="hydE"
                     /locus_tag="PC472_002625"
     CDS             complement(18180..19265)
                     /gene="hydE"
                     /locus_tag="PC472_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2727942.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19301..20509)
                     /gene="hydF"
                     /locus_tag="PC472_002626"
     CDS             complement(19301..20509)
                     /gene="hydF"
                     /locus_tag="PC472_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2727943.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(20524..20775)
                     /locus_tag="PC472_002627"
     CDS             complement(20524..20775)
                     /locus_tag="PC472_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2727944.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         7913 a         2481 c         3399 g         7227 t
ORIGIN      
        1 aagcagaatt ttattaatat ttatatatag atatgaacta ttaataaaac gttaaaaata
       61 caaaaaaact atagaaattg acatatattc cagtatttgc taaagtagct gttggggaaa
      121 attacaaaaa ttattaatgc gcattagata atttgcttta atttttttag catttggggt
      181 aatttataat ataaagcatt agaaaggaaa atataaatga gggacttttt aaaaaattat
      241 aagtcatcaa ttatactttt agcatgtact atagtagggg cgatagtagg attagtactt
      301 ggtccaaaag cttcaatact aaaaccattt ggagatttat tcataaatgc tctattaatg
      361 atagtagtac cattagtttt tcttagtgtt tcttcaggtg ttgctagtat aaaaaatatg
      421 gaacgatttg gaaaaataat gaagggtaca gctatagtat ttatgggaac aacacttgta
      481 attgcatttt taggtattac atctgcatta ttatttaatc cgtctaaagg attagatgta
      541 tcaagtgtaa aaaatttaat ggtggctcaa gatgcagaaa ctttagaatc aaaaggaacg
      601 ttattagacc aagtagtatc aagtattaca gttaaaaatt ttggagattt attttccagt
      661 agtaatttac tacaattagt tatatttgca gttttaattg gaacagcaat agctatgatg
      721 ggagataggg gaaatacaat aagaaagctt ttatcagaag gaacggaagc aatattaaaa
      781 gtagtaagta tattcatgct ttatgctcct atagggctag gctgttatac agctacaata
      841 attggggaac ttggaagtaa aataattcaa ggatatgtaa gggtgtttgt aatttattca
      901 ctaacagcag ccatgtattt cttattattt tacagtctgt atgctttttt atctgcagga
      961 aaacaagggt ttagacaatt ctggagaaat gctataaaac caatagcaac ggcactagct
     1021 acttcttcaa gttcagcatg tataccatca aatttagaag ctagtaaaaa tataggagtt
     1081 cctgatgata tatcagaaac agttatacct cttggaataa atatacataa agatgggtca
     1141 gttttagcag taataataaa agtggcattt ttatcaggta ttttaggtag tggaatggca
     1201 aatccagcaa cgataggtca attatttata actgtatttg ttgttggagt atttatggct
     1261 cctgtagcag gtggctgact tattggtgag atgctcgtgg ttagcatctt cggttttcca
     1321 gtggaggcaa ttccactgtt aacagttata actactataa ctgatattcc taatactata
     1381 ttaaatacaa ctggaaatac agtatcatca atgttagttt caagattagt agaaggaaaa
     1441 gattggctta tagataaaac agcaattact acaaagaaaa taagctaatt gtacacttaa
     1501 ttaataaagt taataggtat cttaatttta ataaattaga tacctattaa ctttttaagt
     1561 aaaaatataa atttaagatt caaatatgat atttataatt tgaatcaact aatatagtat
     1621 atattgaaat aaatatgttt aaatatctat tttatttttt aaaataaaag atttcaaatt
     1681 tatttttgtt aaaagataac aagccttcat ctcgcatctt gccaagctct cttgacatag
     1741 aacttctgtc aacacataaa aaatcagcca tttcatttct tgaaaaggga attgtaaact
     1801 ggtggctacc tgtttgttct atctgttttg aaaaatacat aagcaatttc tcgcgagttg
     1861 ttttacgaga aagtaattct attttattgt gaagtaaaat gtttttttca gctattaatt
     1921 gtagcatatt ttcaacaagc ctattatgaa atacacatgc agatgaacaa ataccagtta
     1981 cacttttaaa ctttataaat aaaacttcgc aagaagatgt tgtggttact gtaacattac
     2041 ttttatgtag tctagtacat gcgaatacct ctccaaataa atctccaggc aagagttcag
     2101 ctacaatatt cctattccct tcgatatctt cctttattat atgagcactt cctttaagca
     2161 taattccaat agaagtaact tcacttccaa cagagaatat tatactatct tttgggtaag
     2221 tcttatctac aacacctaaa cattccaaca tttcttcaag ttcatcactt ttaattcctt
     2281 caaatagagg aattttttta agtaggtcta tatgcttttc taaaaatttt ttcaaataat
     2341 cacttccttg ttgcatatgc aaccgataat ttgttttcat tgtactatac taagtttata
     2401 aaagcaaggg gatataaaaa tcctactaca agaaatttga aataaagaaa tataggtatt
     2461 aaaaataaat atataagaaa tcaatacttt aaattaaaaa aattttatga ggtgatataa
     2521 atgatgagaa aaataattaa aattgatgag gaaaagtgta atggttgtgg actatgtgtt
     2581 gaagcttgtc atgaagaagc tataggaatg gtaaatggaa aagcaaaact tttaagagat
     2641 gactattgtg atgggctggg agattgtctg ccaacttgtc caacaaatgc gatttcattt
     2701 gagtatagag aagcagcaga atatgatgaa gctgcagtaa aagcaaatat ggaagcaaag
     2761 aaagcacaaa agaaaacttt agcatgtgga tgtccaggtt ctcagtctaa gtcaataaat
     2821 agagaggttt caaatagtac gtctataagc aatgatatag tagagataaa aggttctcaa
     2881 cttaatcaat ggccagtaca aataaaatta gtaccaacta atgcatcata tttaaagaat
     2941 gcaagtcttt taatagcagc agactgtaca gcgtatgcat atggaaattt tcataataaa
     3001 tttatgaaaa ataaggttac attaatcgga tgccctaaac ttgatgaagt tgactatgct
     3061 gaaaaactta cagagatttt aaaagaaaat gatattaaaa atatagtagt aacacgtatg
     3121 gaagttcctt gctgtggtgg tatagtaaat gctgttaaga cagctctaca aaatagtgga
     3181 aaaatgatac catggcaaat agttactata tctgtagatg gaaaaattgt agatggagaa
     3241 atttaattaa gtgaattata gaaaaatata ataaatttta ataaataaaa agggaggaat
     3301 aaaaaatgga aaaaatgttt tgttttcaat gtgaacaaac agcaggaggt aaaggttgta
     3361 ctataaatgg tgtctgtggt aaaaaggggg gtacagctaa cttacaagac gaacttacag
     3421 gagtgttgat tggtctttca agagcaacta ttggaaataa gaatagacca acaagtgaaa
     3481 cagataaaat tatgatagag gggctattca ctactataac gaatgtaagt tttgatgatg
     3541 aagctataaa aagacaaatt gaaaaaactg aggtagaaaa aagtaaactt gtacctagat
     3601 gttcagattg ttcaacaact tgtaatagaa atgataacta tgatatgaag gatttatgga
     3661 atgataatga agacatacgt tcattaaaat cattaatctt atttggatta agaggtatgg
     3721 ctgcctatgc atatcatgca atggtacttg gttatacaga caaagacgta aatgaatttt
     3781 tctatgaagg attaatagca gttggaggag acttgtctat tgacgaactt ttaggtctag
     3841 ttatgaaaac tggagaaata aacttaaaat gtatggaatt gcttgataga gcaaatactg
     3901 aaacttatgg aacaccagaa cctacacagg tatctatgaa aatcgagaaa gggccattta
     3961 ttgtagttac aggacatgac ctttatgatt tacacaaatt attagaacaa acgaaagata
     4021 agggtataaa tatatatact catggtgagt tattaccagc acatgcatat ccaaaactaa
     4081 aagaatataa acatctaaaa ggtaattttg gtacagcatg gcaaaaccag caaaaagaat
     4141 ttgataatat tcctgctcca atattattta caacaaactg tttaatgcct gttaaagata
     4201 gttatagaga tagagtattt actacagaag tagttgctta tccaggtatg gttcatattg
     4261 atgaagataa agatttttct agtgtaatag aaaaagctct tgaacttgga ggatatagag
     4321 aagatgttca tatgactggt attaatggag gagatacatt aactactggt tttgcaagag
     4381 gtacagtttc atctgtacaa gataaagtag tagatgctgt aaagagtgga gctataagac
     4441 actttttatt ggttggaggt tgtgatggtg caaaacctgg aagaaattat tacactgaat
     4501 ttgtaaaaca agctcctaaa gacactgtta tattgacact tgcatgtgga aaatatagat
     4561 ttaatgattt agaccttggg gatataggtg gcttacctcg tttaatggat atgggacaat
     4621 gtaatgatgc ttatagtgca attaaagtgg cagtttcact ggcaaatgct tttgaatgtg
     4681 gagtaaatga tttgcctctt agcatggtac tgtcatggta tgagcagaaa gctgtttgta
     4741 tacttcttac actacttcat ttaggtatca aaaatatcta tataggacct tctttaccag
     4801 cattcttatc taagaatgta ttagatatat tggttgataa atttgcattg acacctatat
     4861 ctactcctga agaagatatg aagaagattt taggataaat aaatatatct atacttaaag
     4921 atatttatag ttaaactatt tttaagtatg ccacaattaa gataaggggt taaacttaaa
     4981 atttgattaa aaaatcaatt ttgagatgac cctttattta ttttttgtaa taattgaaaa
     5041 ataaacctgc tttcagtata ttttttttaa gaatctttgt attaaattta taatacttag
     5101 atatataata tcttatatat tttattttaa gtattaaagg tttgaatgta acttaaatat
     5161 taaaagagga acattgtaat atgtaacttg cataaaatat atatactata gtgaaattat
     5221 tttgattaaa atttaaaata taaggtgttt tatgtggggg tatatgtata tgaaaaattc
     5281 taattttaat atattggcat ttgatggtgg tggattaaaa ggggcattga gtatatccat
     5341 acttgagagg atagtaaaag agtatccaaa tctattaaac aatataaata tgtttggagg
     5401 tacatctaca gggagtctaa ttgcactagg gcttgcgtat ggtgtaagcc ctaaagagat
     5461 aaaagaactt tactcaattg aaaattcaaa gtatattttt aataaaagtt atgcagaaat
     5521 actcagacca aagtatgaaa acaaaaactt aaaagaagtt ttattaagta tatttccaga
     5581 ggaactagaa ttaaaagatt taaataaatt agtaatgata ccaagttttt atataggaaa
     5641 tgaggaaaat gcatggaaac cagtgtttta taacaatatg ccaaattctt ttacaaagac
     5701 tagcaaagtt gtagatgtag caatggcaag tagtgctgct ccggtatttt ttccaacata
     5761 taatcgtcat gttgatggag gaataatagc aactgacccg agtttagcgt gtataattca
     5821 tgccattgac agtggattta aacttaaaaa tacaagactt ttttcaatag ggactggata
     5881 tgtatataac agtataaagg cagatactac tgaatgggga gctattgact ggataattaa
     5941 taaagaaccc gatttaccaa ttatatctat taccttagaa ggaaattctc aaatgagtca
     6001 gttattttct caaaaacttc ttggagataa ttactataga ttaaatccta agatggaaaa
     6061 aaatgtagct atggatgatt gtgatgcatt agaatatcta atgtctttag gagaaaattg
     6121 tgatataaaa gatgcttttg gatggataaa gaaaaagagc ttttatagaa attagattca
     6181 atttgcaatt gtatttttac acatgatttc attgagtagg taaaaataat aaaatgcaga
     6241 ttattaagtt acttcttagg gaataaataa agtataattt atttaaggag tgatgaaaat
     6301 gataaaactt gctacttttg caggtggatg tttttggtgt atggtaaaac cttttgataa
     6361 gtatgatgga gttattaaag tagtttctgg atatacaggt ggtcatgttg aaaaccctac
     6421 atatcaagag gtatgttcag gaactactgg tcatttagaa gcaattcaaa taacatatga
     6481 tgatgatata ataagttatg atgaacttct aaatatcttc tggaaacaga ttaatcctac
     6541 agatgatggt ggacaatttg gtgatagggg aactcaatat aagactgcta tattttatca
     6601 tgatgaagaa caaaaaaata tagctttaga gtcaagagaa catctggaaa atagtaaaat
     6661 atttgatagt aaaatagtga ctgatataat tccagcaact aaattctacg aagcagaaga
     6721 aaatcatcag gattattata agaaaagacc attaaactac aatatgtatt ataaagggtc
     6781 cggaagatat aattttgtaa aaagtcattg ggatggaaac aatataaata gggaagagtt
     6841 aaaaaagact ttaactccta tacaatttga agttactcaa aatgatatga cagaagtacc
     6901 ttttgaaaat gaatattatg acaataacaa agagggaatt tatgttgata ttgtaagtgg
     6961 agaagtgtta ttttcttcaa aagataaatt tgattctgga tgtgggtggc caagtttttc
     7021 aaagccagtt atagataaat ctatcattga gagaagtgat ttctcacaag gtatgtacag
     7081 agttgaagta agaagtgcta aaggaaattt tcatttaggt catgtatttg atgatggtcc
     7141 aaaagaactt ggaggtttaa gatattgtat aaattctgct tctcttaaat ttataccaag
     7201 agaaaatatg gaagctttag gatatggaga atatttaaaa ctttttgagt aattaggaat
     7261 atttattata aacttatcta aaaggtgaaa atctaaaaaa atattttcac cttttgtttt
     7321 tttgtcaata gtatgataaa ttattaattg taatcttatt tataccaata gattaagaat
     7381 ttaattttga aagagagaaa acaaatgaat ttaaaagact ttaatggaaa gagattaaaa
     7441 tcagctagaa tttttagagc aaagacgata gaacagttgt ctaaagaaac taaaataaat
     7501 aaaaaagatt taaaagcttt tgaggaaaat aagtatgtac caaatataga aaatacatta
     7561 aaattatcta atatattaaa ttttccaaaa gagtattttt ataaaaatga aaatataaat
     7621 atcgttgtag aggatagcca ctttaaccct caatctaaac tacctagagt agaagagatt
     7681 tcttatagag aaaaaataat tataatacat aaaatatatt catttatgga gaattatatt
     7741 aagtttccaa aagaaaactt gccaaataga aaagtaatgt cagatattaa tataaatgat
     7801 atagaatctc ttgcatataa gactagagag ttttgggatt taaatagtac tccaatagta
     7861 aatatggtaa gtttgttaga gtctaaaggg attataatat caggtatgaa tgtagataga
     7921 aaaggggcaa ctatctttac tcaaaaacag agaattagta aagaatctaa atatctaata
     7981 tctctgggta atgacaaaaa gtcagcatca attagaaatt atactttggc atgtgagctt
     8041 ggatatataa tatcaagtga attaagaata ccaagtaagc aattttcaga agatgagtat
     8101 gcctgtgcat ttttattgcc aaaagagagt tttttaaaag atttgactca tccagaagat
     8161 ttagattatt atgtagaact aaagaaaaaa tggattgtac ctatatctgc tatgattttt
     8221 agagcatata atctagaaaa gataaattac aaaaaataca attatcttat gaatgaaatg
     8281 gataaaaaag gctggttgat agaagaacca ctagacaaaa tgaaaggttc aagcccaacg
     8341 tatttaaaaa gagcagttga attattgata gaaaataaaa taatgagtgt aaatagtata
     8401 gtatcaagtt tagaggaatt tggaataaat ctttatccag aagacttaga attattgatg
     8461 ggattgaaaa aaggattgtt atctcaagag gtaaataaaa aaagtaaagt tataaaattt
     8521 gatggtaaaa aataggttta tattttagta ttaaaattaa aagcaggtac aaatgattta
     8581 aacattaact tagttaaaag ttttttcaat aagtcattta cctgctttta gtgtaagaaa
     8641 gaatattgaa tctaaagtgt ttttgaaagt gcaatatatt cccttaaaac agaacaagat
     8701 ttattaaatt cgttttgctc atattcagtc atattgattt caataatttc ttcaatacca
     8761 tcttttccta taacacatgg aacacctgca aatacatttc tttcgttata ttgaccttct
     8821 agtaatgtag aaactggaag aacttttttc tcattgtgta gtatagcttt tacaatttct
     8881 actaaagcag tacctatacc aaattctgta caatctttac catttattat acttattcct
     8941 gtttttttat ttttttcaac tattgatggt aagtctaaat tgctataagt acttgggttg
     9001 tcttttatca tgtctaagat tggtttacca ccaattgaaa catgagacca aggaaccatt
     9061 tgagaatctc catgttcacc catagaataa gcttgaattg attgttgagc gattccagtt
     9121 tcttcagata aaatccttct aagtcttgtt gaatctaaag ctgttccagt tccaatgact
     9181 ttgtttttag gaaagccaga tttttcccaa atataatttg taactacatc aacaggatta
     9241 gatatgacta caaatatacc atcaaagcca gaatcaacta tgggttttac tatagatttt
     9301 atcattccaa tagttggtct tagtaaatct aatctagttg tgttttgtct acgtaaaggc
     9361 ccttctgaag agtctgcaac acttatcact actatgtcag aatctttaca gtcagaaaaa
     9421 gttccttttt ctatatgtac tttatgtggc agatatgaaa ctgcatcagc taaatctaaa
     9481 gcttgagctt ttgatttaga ttcatctata tcaatcataa agagttcatc acaaactcct
     9541 tgagttatta aactaaatcc acaatgagaa ccaacatgcc ctgaacctac aatagatatt
     9601 tttcttggct ttattgccat tttaaatcac tccccctaaa ataacgatac attgaaaatt
     9661 atagatgtta ttaaaagctg tttttgaata ttttaatatt ctattatttc agaaaattta
     9721 tttaatatga ttttatacta aaatttttaa aaaagcaatg ttaatatact ctctattagt
     9781 gaatattaaa gtattatatg tatattaata attattaaaa tatgtaatgt cactgatagt
     9841 tagaatattt aagtgctaaa agatgtgtat aaataataat gatgtcagct tataatgata
     9901 ttaaattgag atatactgtt tctctaaatt aaaaaataaa ggagtaaatg tgttatgaat
     9961 tatcatacaa aagaagaatt ggtggaagca ctgagagtag tatcttcaag tattattaat
    10021 tgtggaaaag gacagaagaa attttctgag gagacatctc atcatacatg ttttaagaat
    10081 attatagaag taatgtatat ttcaaaatca ttaataatgg atgaaattag taaaagagat
    10141 tgaagcttat tttgcaattt aacatgtttt aatatacatt ttattatata agaaaataag
    10201 taaacataca tgttataaaa tattggaata atatacaatt ataaatttgt taacactaat
    10261 atctatatgt taaaatatag tagaagatgt tagtaaataa taggatgagt tctattttaa
    10321 tacaattaaa ttaagcttag tttgaaattt gtatctaaaa agtaaagtaa tctagaatta
    10381 agaaaatgga gatagaatta taatggaaaa attatactat acaaatcagt atataagaga
    10441 ttttacagca gaaataattg aaataaaaga agtagaaaat aagtatcatg ttttattaga
    10501 taagacagct ttttttcctg gtggaggagg tcagtttgga gacctgggaa aaatagagaa
    10561 tgatgatgta ataaatgtgt atgaagatgg aaaaaaaata taccatgtat tagataaaaa
    10621 acctataaag atacataagg taaagtgtag tattgattgg gatagaagag aagatggaat
    10681 gcatcaacat tttggacaac atgtattgtc tggatgtttt tttaaaactt ttaatgcaaa
    10741 tacgacaggt tttcatttgg gaaatgagta tagtacagta gatatagaag gtaatttaga
    10801 agaggataaa attagagaga tagagttgtt tgccaatgaa attattcgtc aaaatataca
    10861 agtagagaca ttacttccaa cgaaaaaaga actaaaaaag atttggttaa gacgtgattt
    10921 gccaaatact gatgaagaga taagagtaat aaaaataggt gatttagata cgaatgcatg
    10981 ttgtggagtg catccaaaat caactggtga attgagaatg ataaaaataa agaaatggga
    11041 aaaacataag tcaagtacaa gaatagagtt tctagcaggt aaaagggcag tggatgaagt
    11101 attgaagagg gatatatatt tgactaaaat atgtagatat ttaagctgta gtgaggaaga
    11161 tgctataaat ggtattgtaa atctaaatag taaagtggaa gaggctttaa ttaaaaaaag
    11221 aaggcttgaa gaagtagtat ctacatatca ggtcaaagaa atgattgaaa aagcaaataa
    11281 ggttggcaat atttcggtca ttaaaaaaat ttatgatgat gaagatttga aatatgtaaa
    11341 taagatagca aataaaattg cagaatatga aaatagtatt gcattattag ctgttaaatt
    11401 taatgataaa ataaatttag tttttgcatg ttctaaaaat ttaaaagata ttaatatggg
    11461 agttttatta aaggatgcaa taaatcttat agatggcaag ggtggtggaa gtaaagtatt
    11521 agctcaagga ggaggtaaga ataatggaaa tttagaggct acacttgatt atgcatttat
    11581 gaagatagaa aaaactctat aaatatatta tataaaaaac cttagtagat aaaatagata
    11641 tggaggaata agtatggagc aacaaaagga ctacatagag ttatatgaaa atgaagaact
    11701 aatagagata agagaagcac gtatggatga tttggataca atagcaaaat ttaattataa
    11761 tttggctaaa gaaactgaag gaaaagaact tgatatggat gttttaacta aaggtgtaaa
    11821 agcattgtta ttagatgaga gaaaaggaaa atatcatgta tatacagtat ttgataaagt
    11881 tgttgcacag attatgtata catatgagtg gagtgattgg agaaatggaa atttcctttg
    11941 gattcagagt gtatatgtag ataaggaata tagaagaaaa ggtatcttta attatttatt
    12001 taattatata aaaaatatat gtgataaaga tgaaaatata gttggaatga gattatatgt
    12061 agaaaaagaa aacataaatg caaaagcaac atatgagtct ttaaatatgt atgagtgtga
    12121 ttataatatg tatgaatacg aagtaataca ttcgtaatat aaatttgtaa tactgttata
    12181 ctgatgttat aaagtattga ttatattgac gattatatga aataaactgg atattataat
    12241 tagaattttt agggggtata aaaatgggcg ttttaaaagg aatgttttat gtatttttat
    12301 ttttagactt agtgtcaata ttctgtttct ttttcaataa aggaaaaata gcatctaata
    12361 aaattgtgtt taatgcaata ggagtactta catttgtact ttgttttatg ctttttagtt
    12421 attatcctaa taataatctt attggaaaat ttattgcttc tctatttttt atatttggag
    12481 ttgcaggagt tatgttgaaa gaaaaaaatt ttttatatgc tagacttctt ttaactgtag
    12541 ttatagtttt ttctacatta agattgttcg ttattcaata aaatgtattt gtatgaaaat
    12601 ttattaaagt ctacacattt aagtgtaggc ttttttgatt ttttgtttga aaattaagga
    12661 aattttaagg aaattttaaa atcgtcttaa atatttattg atatacttaa aatattaata
    12721 gcgaaaggag acttatcagt gatagaaagt aaaaagtcag aaaaattgtt actgtcaatt
    12781 atatacataa tatttttggt atatatttta atgattataa gaataatttt atttagagat
    12841 gtaccaattt acgctatttt caagggaacg tttaggaatg taaatttaat tccattttat
    12901 acaatatatc aatttatagt agatagtaat cttgacttta tgaaagctac tgtaaatatt
    12961 atagaaaata taggtatatt tattcctatg ggaatttttt tacctatagt atgtaaaaat
    13021 ctaaataaaa aaactataat tatagtaatt atacttgtta gcctagcatt tgaattaaca
    13081 caatatattt ttgctcttgg aagtagtgat atagatgatg ttattttaaa ttcattagga
    13141 ggcataattg gaattaatat ttacataaac atgaataaat tatttcccaa tgatattaac
    13201 agatttaagg ctataatagg tactagttta gttttaggag ttataggatt aggagttatt
    13261 tctaaaaatt atcataactt actaactttt aaatttagac ctgatagaaa aatatcaaaa
    13321 atattaattg aagaaaatag ggaaataatt aaagatataa ataaagatac agtagatata
    13381 gttgggacat ttgagagctt taaaaaagga ataataacta ttaaagcagg ttctaataac
    13441 aaagtaaaga gaccagagaa tttaattgat tctgatggaa atataagaat ttatttaaat
    13501 gaaaatacaa aattagtatc ttatataatt accaaagaat caaaaatgga tatagttaaa
    13561 tatgaggaat ttgatgtgaa gaatttagat ttattaagga gatatgatac tattaatgtt
    13621 tggattgata aagaaaatca gccaaaggat aaacatggta ttatggctag taaattatta
    13681 attggattgt atgaataaaa tattaaacaa gaataaaaga ataacaaata taaaaaacga
    13741 aaaaactatt tctataatta tttttacgtt gtgcataaaa taagaacatt agtgaaggag
    13801 tagagataaa ttatggagat aataaaatat attttgtact tttttatata ttcattttta
    13861 ggatggacag tagaaagtat aggatgttct atagcaagca aaagaataat caacagaggt
    13921 tttttaaatg gtccaatatg ccctgtttat ggttttggag cggtaattgt gatatcacta
    13981 ttgggaagat ttaataatgt agtaatagta tttctattag gaatgatttt aactacgata
    14041 ctagaatatt ttacaggatt tattttagaa accttatttc atgctaaatg gtgggattat
    14101 tctgacagaa aattcaatat aaagggtaga gtatgtttaa aaaatgcaat atactttggt
    14161 gtaatgtctg ttttaataat aagatttata catccattta taaaatattt tgtaagtata
    14221 attccatata gaatattaat ttcaatggct ataattacaa cactttggac tatacttgat
    14281 ttgattgtta ctattataac acttaaaaaa ttagatataa agttaaactt attagacgat
    14341 ataattacag accttaatga tataaatgtg aaattggata aatttgatag aggagaaata
    14401 caagctttat ttaaaacaat aaataacgac gaattagaag ttagagaaaa aataaacaaa
    14461 ataaatagta agttggaccg tatagagtct aatattattt tacaaaaaag agttataaaa
    14521 gcttttccac atataaaaca taaaaaacaa caagagcaac tagaacattt taaaaaaatt
    14581 attaatgaga aaaggtaatt atacaattta atgaagacga atagaataat ggtatttata
    14641 agatgatttt cagaggactc tataaaaata gaatagagtc ctttattttt tgaaaaataa
    14701 tgtttatttg ataaaaataa aatatattgt ttctgaataa caaatatttt gaaaattata
    14761 aaaacatata ttgacaattt gtgtcaaaaa tactaacatt aagtatataa agaattttat
    14821 atgagaatat ctttataaat cgcaataatt ttaaaacata aatttattta aagaggggat
    14881 gatttgccaa tgagcgagat aactagtcaa atgataagta cagaagaaaa aaaatatgtt
    14941 gctaaaactc aaaagatacc ttattatcca gtagcattta aatcaggtga tggtgcaatg
    15001 ctatatgatt atgaaggtaa tgaatatgta gattttttag caagtgcagg ctctgcaaat
    15061 gtaggtcatg gtaataagga aatctcacaa gcagttaaag aacaaatgga tgatataaca
    15121 caatatacac ttgcatattt ccatagtgat cctcctgtaa aattagctga gaagcttgtg
    15181 gaaatagccc ctggagataa tgataaaaaa gtactataca gtgcaactgg ttctgcttgt
    15241 atagatgctg ctataaaatt agcaagaggg tatactggaa gaacaaagat aatatctatg
    15301 tgtgaatctt atcatggaag tacttatgga gctatatcta tatctgcttt aagtacaaat
    15361 atgagaagaa aaatgggtcc attgttacca gaagtttatc acttccatta tcctgataaa
    15421 aatagaacag ctaaagaatg tttagatgag atagaatatg cttttgcaca ctatctacca
    15481 gcagaagagg tagctgcaat atttatagag ccaattgcag gagatgcagg aataatagtg
    15541 cctccagtag agtgggtaca aggtttaagt aaaatatgta aagaaaatgg aatattactt
    15601 gtaagtgatg aaatacaaca aggtatggga agaacaggta agtggtttgg tatagaaaat
    15661 tttggtgttg aagcagacct tatagttctt ggaaaatctg ttggaggtgg attacctcta
    15721 ggagcagttg ttggaagaac agaaataatg caaagtcttg atgcaccagc acatttattt
    15781 actttagctg gaaacacaac agtatgtgtg gctgctttaa aatctataga gataattgaa
    15841 aaagaaaact tacttcaaaa gagtatagaa atgggagatt atattaaagc aggatttgaa
    15901 aaactaaaag aaaaatacga tataattgga gaaataagag gcataggatt atctatagga
    15961 gttgatatag ttaaaggaaa aggttctaat gaaaaacatc cagatgctac agctaagata
    16021 tgttatagat gtatacaaac tggattaata atgatattct taggacaatc tactttaaga
    16081 gtgcaaccac ctctagtaat aactaaggag caagtagata aagcaatgaa catcatagat
    16141 tctgcaatag atgattattt aaatggtaga attggtgatg aagtatatga agtaactcaa
    16201 ggttggtaaa ataaatatta aatgaaaata ggagttatct caattttaga aaattatttc
    16261 tgaaatgaga taactctttt aagttaccca caaataagaa aattgtttga ttcattaaag
    16321 actatatttc ggatgtcgtt tttgtgagta tcagagacag tattacgaga ataattacct
    16381 ttattcgtac tatctgttaa tttagttata attaagatag ctaaaagaat aagaattact
    16441 gtgtataaag caataacata tggttttctt ttatatttgt atttgttatt gaaaaaagaa
    16501 tggccataat tatttttatt tactttagat gtatttgtat tctttttctt gttacctttc
    16561 tttttatcat ccttttttct atttttaggg taaggaatga ctttgtgtgt attatcaagt
    16621 ttgatatttt tatttttttg aatattgttt tccaggctag cctttttttc tccaaattga
    16681 atagttttat tggtattgtt gttatctttc aatgttaatc tccctccctt aaactttccc
    16741 tttagataat attactacta acctatggaa tatacaataa ataatatata agtaataaaa
    16801 ttttataggt ataaatctct ttctccacgc tctaaggcag tcatattgtt agatactatg
    16861 tttcttatat cttctctttc tatcttacct atttcttctg cgataagttt tttacccata
    16921 attttaagtt cttcatctcc ataatcgatt aaaaattcat ttaatgtagt aagagcattt
    16981 ggtgtacata cattatggat ttgtcgactt ttagctaagc tcatgaatct atcgccagtt
    17041 ctacctttac gataacaagc tgtacaataa ctaggtatat atccatcagt aatcagttct
    17101 ttaagcacct caacagggct tctatggtct ccaacttcga attgttctgt attgtcacca
    17161 tcttcatatg ctttatatcc tccaacacct gttagagaac ctgcactaat ttgtgataca
    17221 ccatatttta gtaactcatt tctcatttca gcagtttctc tagtggacat tatgattcca
    17281 gtaaaaggaa cagctaatct agttatagct actatttttt tgaacatatc gtcagaaact
    17341 aagtgaggaa aatcttctaa gctcatacct tcagcttttt tcaatctagg gaaagatata
    17401 gtatggaaac ctacaccaaa tttttcctct aagtgttcgt tgtgcatcat aagtcctaga
    17461 acttcaaatt tagggtcaga taaaccaaat aaaactcctg caccaacatc atcaacacca
    17521 gcttccatag ctctatcaaa agctgttaaa tggtaataat agtcattttt tatagattga
    17581 ccatgcattt ttataaatgt tggtttgtga taagtttctt ggaataatat ataagttcct
    17641 attccttttt cttttagtaa tttatactca tctacacttg tagctgctat gttaacgttt
    17701 acccttctta tatttccgtt ttcattatat gttgaataaa ttgcatctaa gcaatctaag
    17761 atgtaattta tatcacaatt tttagggtct tcaccagctt ctagagcaag tcttttatga
    17821 cccatttttt ctaatatttt aacctcttct tttatctctt ccatggtaag tttttttctt
    17881 ttaaatttgt tgcatttact aaaaccgcaa taaacacatt catttacaca gtagtttgat
    17941 acataaagag gggcaaataa aaccactcta ttaccatata tttcattttt tatttgccca
    18001 gctatactaa ataatctatc tagttgtttt ttatcttcca cttcaagaag tgtagctata
    18061 tctttataac taagtttttc ccttctatca gccttatcaa gtaccttttc aatatcatca
    18121 gaagttgaat tttttgcatc ttctaataaa gaattaataa gttcatggtt tatgaacatt
    18181 tacaaacgcc tccattcaat attatcaccg cgagaatagt caacttcaag tcctaatgac
    18241 ttgatgtttt cttctaaact ttggtgatat tcacaagctt cttgaccagt aaaggctttg
    18301 ttatcgtaaa gagaatattg ttttctaaat tcttgaggag aaaggtttgg cataattaca
    18361 ttgcaaccag ctaaaagacc tgcatttcta ccactagggt ttatagatgc caaggctgtt
    18421 gtagcgggta ataaaacttt tggaagtaga agtcttgtaa ttgaaagcat aagtaatgtc
    18481 ttctctaaat ctccatgttt ataatcctta aaaattgtat catggtgtgg aatgaaaggt
    18541 cctattccaa ccatatgagg atttaactct tttaggtata ataaatcacg aacaaggtct
    18601 tcgttgttct gaaatggtga ttcaaccata aatccagcac ctacttgata accaatttct
    18661 ttaaggtttt ttaggcattc ttttctagtt ctaagctcaa tgtttggcgg atgtaatttt
    18721 ttataatgac tgtctgtagc agtttcatgt cttaaaagat atctatctgc accacataaa
    18781 aagtattttt tatacgaatc ataactcttt tctcctagtg atagagttat ggcacaatcc
    18841 tcatatttat tttttatact tgaaacaatt tcacatattt tatcatcagt aaaatatgca
    18901 tcttcaccac cttgcaaaac gaatgttctg tatcctattt tataacctat atcacaacat
    18961 tcaagtattt catcaagtga aagcctgtat cttatgatat ttttattaga agattgtatt
    19021 ccacaatagt agcaattatt tttacaatag tttgtaagct ctataagtcc tctcaagtag
    19081 actctatttc cataatgttt atttctcatg tcgctagctt gtttataaag atagtttctt
    19141 aaattttgtt cgaagttata ttcctcattg tcgatatttt ctattaaata tagtagttca
    19201 tcaaaactta atgaattaat gctgtaaagt ttatctataa agtatttaat cttattttta
    19261 gtcatattca aatatgtaat tattaagtat agttaaattt ttaataattg aaaagttcaa
    19321 gtgatctttc caaaatacct tttacatatg ctattaagac accatagttt acaataggta
    19381 cattgaaaga ttttgccttt tctattctag ataacattcc agctctattc atcatacaag
    19441 ctccacagtg aactacaagg gcatatttat ttatatcttc agtgaaggat actccagaac
    19501 taaattcaaa agtaatgttt ttgccagttt tctttctaat catattagga atttttactg
    19561 ttccaatatc atcagtttgt ctatgatggg tacatccttc ggccatcaag attttatcac
    19621 catcttttaa attttctaaa gcataagctc cattaatcaa ttctttaagg tctccctttt
    19681 gtctagcaaa caatatagaa aaagatgtaa gaggaatatc ttttggtgta tctttatcta
    19741 cttgtggaaa tacttgagag tcagtaataa caagttttgg ttttttgcct aaattagata
    19801 gagtttcttt taaactatct tcttttgtaa ctatagaaat tgcaccatta tccaaaatat
    19861 ctcttataac ttgctgttga ggaagtataa gtctaccttt tggagctgct ttatctattg
    19921 gaacaactaa aacaactaaa tcatttggtt caattaaatc tgataccaat ttgaactcag
    19981 tgctatcctt aggcaaaact tttattattt cattttttaa attttcaatt ccaattttat
    20041 ctgttgatga gacagaaaca actggacatt taactttatt tttagttaag tttaaaattt
    20101 ctgattgatt cttaatagta tcaattttgt taagaattag aatatgaggg atattcttat
    20161 cattaaattt ttcaattagt gacaaatctt cttgtgatat accaatttgg caatccacta
    20221 ctagtaaagc tatatctgtc ttttctaaaa catctagtga tttacctatt ctaagttcac
    20281 caagttcacc aacatcatca agaccagcag tatctataag aacacaaggt cctataggaa
    20341 ggatttccat tggtctaaat actgggtctg tagtagttcc agcaatatct gagactattg
    20401 cagcactttg atttgttata gcatttatta tgctagattt acctgcattt cttttaccaa
    20461 agagacctat atgtactctt accgattgtg gagtagaatt tagactcatt tgtactcacc
    20521 accttaaact tctttttttg atatactagt ttttacagat acatgaggta tgttgcctaa
    20581 tttaccagtc aaactattaa tagtatccat ttcgccaaca actgctatac atacgattga
    20641 gacaccttct tcttcgcaag gaatgcccat tcttccttta ataatacctt taaaacctga
    20701 tacaacctca ttgaatttaa attgacattc ttttggctct tccaaaatag cacttataac
    20761 tgcaaccttt ttcataaata tcctcctaac tcagaaaata tgtcttaaac aaatccaaaa
    20821 tcaaaaaact tttctttgta aagaaaagtt tctacaaaag aaaagttatt aattacacaa
    20881 gtataatgtg ctatattaaa ctatttcctt acagattaga taactctttt ctggtagggt
    20941 tagcttgtaa aaatttaatt taacaacaag taactgaaga aaatttttat acaatttata
    21001 taaattataa taaaaattaa
//
LOCUS       DAMJYC010000065        20934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJYC010000065 DAMJYC010000000
VERSION     DAMJYC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.066"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            71..892
                     /locus_tag="PC472_002628"
     CDS             71..892
                     /locus_tag="PC472_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2727945.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            913..2109
                     /locus_tag="PC472_002629"
     CDS             913..2109
                     /locus_tag="PC472_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2727946.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2276..5674
                     /locus_tag="PC472_002630"
     CDS             2276..5674
                     /locus_tag="PC472_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2727947.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5684..7357
                     /locus_tag="PC472_002631"
     CDS             5684..7357
                     /locus_tag="PC472_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2727948.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINNDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7630..7824
                     /locus_tag="PC472_002632"
     CDS             7630..7824
                     /locus_tag="PC472_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2727949.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7993..8226
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8303..9700
                     /gene="asnS"
                     /locus_tag="PC472_002633"
     CDS             8303..9700
                     /gene="asnS"
                     /locus_tag="PC472_002633"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2727950.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9873..11048)
                     /locus_tag="PC472_002634"
     CDS             complement(9873..11048)
                     /locus_tag="PC472_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2727951.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11467..12831
                     /locus_tag="PC472_002635"
     CDS             11467..12831
                     /locus_tag="PC472_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2727952.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12853..13344
                     /locus_tag="PC472_002636"
     CDS             12853..13344
                     /locus_tag="PC472_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2727953.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13617..14288
                     /locus_tag="PC472_002637"
     CDS             13617..14288
                     /locus_tag="PC472_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2727954.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14373..15239
                     /locus_tag="PC472_002638"
     CDS             14373..15239
                     /locus_tag="PC472_002638"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2727955.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15272..16774
                     /locus_tag="PC472_002639"
     CDS             15272..16774
                     /locus_tag="PC472_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2727956.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16904..17356
                     /locus_tag="PC472_002640"
     CDS             16904..17356
                     /locus_tag="PC472_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2727957.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17516..18319
                     /locus_tag="PC472_002641"
     CDS             17516..18319
                     /locus_tag="PC472_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727958.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18377..19264
                     /locus_tag="PC472_002642"
     CDS             18377..19264
                     /locus_tag="PC472_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2727959.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19283..20131
                     /locus_tag="PC472_002643"
     CDS             19283..20131
                     /locus_tag="PC472_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2727960.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20194..20883
                     /locus_tag="PC472_002644"
     CDS             20194..20883
                     /locus_tag="PC472_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2727961.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8218 a         2221 c         3753 g         6742 t
ORIGIN      
        1 atttttatat caaaaatagt aataaagtct atatacattt agaaaatact gtttaaaagg
       61 agtaggattt atgataaaaa aagagatatc taactttaga ggaagtagtg attatgtagt
      121 atcaccagaa ttgatggcat cagttaatgt tgctattgct cttgaaaagc cacttttgat
      181 taagggcgaa cctggaacag gtaaaactat gctagcacaa gcaatttcaa atgaattaaa
      241 aaaagattta gtaatttgga atataaaatc aactacaaaa gcacaagaag ggctttatgt
      301 atatgatact gttcaaagac tttatgatag ccaatttgga ggagaaggtg tagatgatat
      361 tagcaagtac ataaaatatg gtaaactagg agaggccttt agctcaaatc aacaagtaat
      421 tcttttaata gatgaaatag ataaggcgga tttagaattt ccaaatgact tgctttggga
      481 attggataaa atggagtttt acataaatga gacaaaagaa actgtaagag ctaaacaaag
      541 accaatagtt attataactt caaatgcgga aaaagaactt ccagatgctt ttttaagaag
      601 atgcatattt cattatatag aatttcctga tagagacatg atggaagaaa tagttaaagt
      661 acattttgat aaagtagaag aacacctatt ggaacaagta atgactactt tttattggat
      721 tagaagttta aaggacatac aaaaaaagcc tagtacatca gagttaatag actggataca
      781 agcattaact ttgagtggaa tgcctattga aaaaatagaa aaagaagttc cttttgcagg
      841 aatactactt aagaataatg aggatattga atctatgcaa agacacttat agttaatagg
      901 agctggataa ttatgtttat tgaatttttt tatttattga ggtcaagagg attaaatgta
      961 tctttaaatg aatggatgac tttaattgaa gcattggata aaggactgtg ctattcaaac
     1021 ttttcaaatt tttactatct ttgcaggatg attttaataa aaagtgaaag tgattttgat
     1081 aagtttgatg cagtgttttt ggagtatttt aaaggtatag aacaccaaga agaaattcca
     1141 gaagagatta tgaattggct taaaaaacca gatatagatt tagaagagtt tgaaagatta
     1201 gaacaaaatc caaatataaa tcttgataaa ttaagagcta aattggagga acgaataaaa
     1261 gaacaagatt ctcctcataa tggtggtaat tattggatag gcacaggagg cacttcagag
     1321 ttaggacatt caggaaaagg gcaaactggt atacgtatag gtggaaattc tacttatggt
     1381 agagctgttc ttgaagttgc aggagaaaga aaatacagag attttagaga tgatgaagtt
     1441 ttaaatatgc gtcaatttca agttgcacta aagagacttc gtcaattttc tactagaata
     1501 gatgctccaa agacagagct tgatttggat aagactattg aagagacttg taataatgca
     1561 ggatatttaa agttattttt tgagaagcca agaaaaaata cagtgaagct tttattgctt
     1621 atggattcag gtggttcaat gagagggtat agcactttat gtaatacatt atttcagtct
     1681 gtaagtaaat caaatcattt taaagatgta aagatatatt atttccataa ctgtatttat
     1741 gatagattat ttacaacacc tgaatgttgg ttaagtaaat ctattaatac tgaatggata
     1801 ttaaaaaata tagataaaaa ctataaagtt ataattgttg gagatgcttc tatgtcgcct
     1861 tctgagttgc ttcatattgg tggtaactat cgtggtccat ataattatac acctggtata
     1921 gagtggctta aaaggtttaa gagaaagtac agtaaaattg tatggatgaa tccagaactt
     1981 agagatggat gggattcaat tagttattgg tatcaaactc aaagaatgat acaaagtgaa
     2041 tttaatatgt ttccattgac tgtaaaaggt cttgagaaag cattgaaaaa tttaatggtt
     2101 tcaagataaa gtaaatataa atgatggtat gatttattca aaattgtacc atcattattt
     2161 atttttttaa tatatctatt ataaattgta taactgaaac ttaaaaaatt atatagtcta
     2221 atacatttta gactcacaca aaacatatac attaataata attttagaag tcgaggtgat
     2281 tataataaat tatgaattaa ttgtaaaata caatggagat atattgaggt tagaagaaga
     2341 attaggtgta tcagtagaga tacttaattc atcatatgca ataataacat caagtaatga
     2401 agaagatgta aatatacttt taacttatcc agaaattgaa tttatagaaa agccttttat
     2461 attacagact caggatgtgc aaagtttttc aagtacagga ataacaggat ttaaaaatag
     2521 aactggttta actggaaaag gaactataat tggtataatt gattcgggaa tagattatac
     2581 tctacctgtg tttagagata gtgatggaag gtctaagata ttatattatt gggaccaatc
     2641 tattcaagga aatccaccag aaggttttag agaaggaaca ttatatacta atgaagatat
     2701 aaataatgca atagatggaa gtatgtatat acctatttca acaactagct tacatggaac
     2761 acatgttgca ggtatttgtg caactattgc aagtgatgcc agaataatag tggttagagt
     2821 tggaaatata cagaccgata ttttttcgag aagtacagag tttatgaggg caattaaatt
     2881 tattttagat agggcattag aattaagaat gcctgttaca ttaaatataa gttatggaag
     2941 taatgaaggt tcacatagag gaacatcttt atttgaacaa tatatagatg atatgtgttt
     3001 attttggaaa aacaatatag ttgtagcagc tggaaataat gcagataaag gaggtcataa
     3061 aaggattagg cttcaaaata atattacaga agaagttgag tttatagttg gggaaggaga
     3121 gcgtatatta aacataaata tatggccaga ttttgttgat gattttagtg tacatttggt
     3181 aaacccatca aataatcaaa cacaagcaat ctctctaact tcaggtgaga taaggaatac
     3241 tttgggtgaa actagaataa ctggttattt ttaccctata gcaccgtatt ctctcacaag
     3301 aagagttact ctacaattaa gttcaaatac acagataact cctgggttat ggaaaattgt
     3361 atttgaacca atagatatag taactggtaa tgtaaatata tatttgccaa cttctgaagg
     3421 tctaaataga aatactagat ttctgattcc tactcaagag cttactgtaa cagttcctgg
     3481 aacagcaagt agagttataa cagtaggtag ttttaattca agaacagata tagtatctat
     3541 attttctggt gaaggagata cacaattagg tgtttttaaa cctgatttat tagctccagg
     3601 tgaagatata gtatcttttt taccaggagg tacaagtgga gcattaactg gaactagcat
     3661 ggctacacct catgttacag gggtttgttc attgtttatg gaatggggaa ttgtaaatgg
     3721 aaatgattta tttttgtatt cacagaaatt aagggcatta cttctaaagg gagcaaggag
     3781 attaagtaat caatcttatc caaataattc atcaggattt ggttttttaa atttatcaga
     3841 tatagatttg tacactctat ctaatataaa tcaagattta gaaacagagg atattggata
     3901 tagaagtatt aataagtcat ttaaagatga ggaaaatagt tataagttta tagatggtta
     3961 taatatgcaa atacataatg acttagagaa tgaaatttat ataagtaaaa atgcaagtag
     4021 acaatcagga atactatcag gaatagatat agtccatact cctgaatttg aagaagaact
     4081 agctgggtta ggtatgagtc aaaggttttt taagataagt gattcattag gtgtactatc
     4141 tataaataat acagactata atagcataca acgagttttg caacttcctt caatcataag
     4201 aactgtatcg actacaaaga tgacattact tggagaaatt aatcgaggta cttttggagg
     4261 tgtagttgca acagaagaaa tgggcgtaaa tttctttaaa aataatccta atattaatat
     4321 aacaggaaga gggactctaa tatcaattgc agatacagga atagactatc tacacccaga
     4381 ttttatttat ccagatggca cttcaaaaat agtatattta tgggaccaaa caaaagaagg
     4441 aactcctcct gatggttttt atattgggac agaatataca cgagaagata taaatcgagc
     4501 tatcgcagaa aatgacccta gtctatctca ggatgaagtt ggtcaaggaa ctatgttgag
     4561 tgggatatgt gcagggcttg gaaatgtaaa tagtgaatat gcaggtatag cagaagattc
     4621 tgagctaata ataataaaac tcggcaagat agatgatttt tataatagtg caatgttgtt
     4681 tgctgcatct cagtatgcat ataaaaaagc ttttgaactt ggaagaccat tagttataaa
     4741 tatgtcttta ggtaccagta gcctagcagg tttaaccaat aggagtaata gtgaaaaagc
     4801 gttctttact agagggttat gtataacggc aggagcagga aatgagggca atacacagac
     4861 acatacatct ggaataatac catatgttgg aggttctgtt gaagttgaat tagagttaaa
     4921 tgaagacgag gaagaattat cattagaact ttggttaaat agaccagata aagctgatgt
     4981 aataatagta tctcccactg gtgaagaaag taaaagtgtt ggaatttcaa attataataa
     5041 agtaacaggt ttatttgatt tagaggggac tgaatattct ataacatata tatacccaac
     5101 aactttttct ggacagcaat ttactaatgt tactttaaag aatgcaaaaa gaggtgtatg
     5161 gaagattcga ttagtaggag tttatattat aacaggaaga tataacttat accttccaaa
     5221 tagagaactt ttaaaaagtg gaactagatt tagagaggta gacccttttt acactataaa
     5281 ttatcctgcg attcaagatg atttaataac cataggagcc tacaatacca ttaatggtag
     5341 tttatggcaa agttcatcaa gagggccaac tattgaagac aggttaaaac cagatatagt
     5401 agcaccaggt gtcaatataa tagctgctta tcctgggaac acgtatgcaa ctataactgg
     5461 tacagcagca gcaagtgcac atgcagctgg tgctgctgca atgtattttc aatatacttt
     5521 tgttgacggt agatatccaa accaagcata tgtgcaaaaa ataaaaactt ttatgcaagc
     5581 tggtgctaga aaagattcaa acacagtata tccaaataca aattctggat atgggctctt
     5641 ggatgttaga ggaatgtttg atgttttaag ataggaggga tttatggaaa aatcttattg
     5701 tataatttat caaggtgata tagaatctgc cctacaagaa aatggaataa ataggtatat
     5761 ggttttaaat agtcagcttg cagtaatata tgtaccttta gattttgatg aaactatttt
     5821 aaataatata atacaagttg cttggtggga agaatctgaa ccaatgagca gtctaattga
     5881 aataactaat aatgtaaata atggagaaac cattacaaca gcagctgaaa ctgattatat
     5941 ttatgaaaat ccatataatg atataacagg gagaggaatt ttattagcag ttatagattc
     6001 tgggatagat tatttgcatc ctgattttat aaataatgat ggaactagta aagttttata
     6061 tttatgggac caagaagcaa atacaaatcc acctccagaa ggttttattt ttggaagtga
     6121 atttacaaga agtgagttaa atatagccat aaatagaaat gatgggagtt tgagtcaaga
     6181 taatataggt actggaactt tagtttctgg tatattagtt ggaaatggaa ggataaattc
     6241 tcagtacaga ggtattacta cagaatcaga cttaatagta gttaaattaa aatcatatac
     6301 agatacttat tatgctggaa ggataaatta cagtgtttca gattttttag cagctataac
     6361 gtatgttact aacatagcaa gaacagaaaa taaaccactg ataataaatt tgactattgg
     6421 agttaagtca agtgctgtag caactacttc aatactagat acttttaata tattgagtag
     6481 tgctggtgtg gtagtagtga gtggtgcagg aaatcaaggg aatacagata ttcattattc
     6541 tggcagattt agcagtgtgg gagaagttca agatgtaata atacaagatg gagatgacta
     6601 tgctcttgat ataacactaa atacgaatgg tccagataaa gtgggagcac agataatatc
     6661 tccttcaggt gaagttagtc atgatataag gtattctcca gacttttata tatatagagg
     6721 aaaatttaac ttagaaaata ctacttatgc aatgagattt atttatccat atattacctc
     6781 tggaaaagaa aatttggaga taagattaag agatataaaa cctggagtat ggattttaag
     6841 attgacttca gaacttataa taaatggaga atatgacata tacttgccaa ataaaaattt
     6901 gatagctcca gacacaaggt ttttagagcc tgattcagta gctactataa ctatgtatgc
     6961 tgctagtgat gatgtaatta cagttggtac atttaacaat aagactgata gtatgtggat
     7021 tggttcttcg aaaggaccaa ttagaggaag aggaattaag ccagatatag tagcttcagg
     7081 tgtagatata atatccacat ataaaaatgg aacatataat acaggaacag gaacaggagt
     7141 gagcagttct atagtcacag gtgttttagc cttattgatg gaatacttag aaaaacagga
     7201 taatgtaccg agattatcat tgtttacaca agttttaaaa acatatttaa tattgggtgc
     7261 tacaaaactt gagatatata catatccaaa tgtatcgcag ggatatggga ttttaaattt
     7321 aaaaaatacg attcaacaga tagcaaatac tctatagata agaacctatg taaaattata
     7381 attgattatt agtttaattg tatcaaatac tttagataga gtagaaaaat agcataaaaa
     7441 ataaatatga aaattaaaaa acagttttat attttgaata cacaaagtaa atattttgta
     7501 tataaatagg tataaattaa gtattgaaaa gatattatgt taatttaaaa ataattataa
     7561 tttagactta tgaattttat ttgtaaaatt gctaaaacta ttacaaaata aataatagag
     7621 gtgatagttt tggaaagtaa aaaggcaaga gcattaaaag gtaaagataa taaaagactt
     7681 aaaagttcac ttttagttgg aaatgagtcg acagcagcat gggctgatgt tgaaaaatta
     7741 aaacctcata gtaaagtttc aataccttca attaagggtg tcgaagaagc aaaagactgg
     7801 gttgacaatg gaagtaaatt ataaaatttt ggaaattgaa gtaataaaga agtaatgaag
     7861 ataagtattt attatcatta tgtaaggtag aattattata ttttaaataa caatcagtat
     7921 caattatata aatttattga ctaaaaattt ataaattggt ataattttgt taattagcag
     7981 aaataataaa agataaatac tgtgaagaga agagtagata taaaatgtag ttctagcgag
     8041 ttgaggatgg tgaaagctca atacgaggtt tatattgaag aacatcttgg agtttctaat
     8101 cgaaagctta tttagtaagt agacttagac ggaaggcacc gttataggct ttaagcattg
     8161 cttaactaag aggtcattat gacaactagg gtggtaccgc gagagtaata gtctttcgtc
     8221 cctttatata gggatgaagg gcttttttta ttgaaattat atttaaaaga aattaataac
     8281 aattatacta ggaggattta aaatgcaaaa ggaatttata acagtaaaag aattgtacag
     8341 agaaaaagaa cagtacatag ggaagactgt aaaagtagct ggatggataa gaacatctag
     8401 agtatctaaa aattttggat ttatagagct aaatgatgga agcttcttta aaaatatgca
     8461 gatagtattt gatgagaaag tagaaaattt taaagaggta ggaaaattag caataagctc
     8521 atctatactt gtagagggag agttagtatc tacagaaggg gctaaacaac cagtcgaaat
     8581 acatgcaaaa agcatagttg tagaagggga atcagatagt tcgtatccac tacaaaagaa
     8641 gagacatacg cttgaatatt taagaacgat agcacattta agaccaagaa gtaatacttt
     8701 ttcagctgta tttagagtaa gatcaatagc tgcctatgca atacacaaat tcttccaaga
     8761 aagaaacttt gtatatgcac attctccaat cataacaggt agtgactgtg aaggtgcagg
     8821 agagatgttt agaataacaa ctatggattt aaatgatatt ccaaaaactg aagatggcaa
     8881 aatagactat actcaagatt tctttggaaa agaggctaat ttaacagtta gtggtcaatt
     8941 aaatgccgag ataatggcac tttcttttag aaacgtttat acttttggac ctacatttag
     9001 agctgaaaac tcttatacag gaagacatgc ttcagaattt tggatgatag agccagaaat
     9061 agtttttgca gaccttgaag ataacatgga acttgcagaa gatatgataa aatatgttat
     9121 aagctatgta ttagaaaatg ctcctgaaga aatggaattt tttaatagtt ttatagataa
     9181 aggattgtta gaaagattaa ataagatagt aaactctgag tttattagaa taacgtatac
     9241 taaagcagtg gaattattaa ttgaatctgg gcatgaattt gagtatcctg ttgagtgggg
     9301 atgtgacctt caaacagagc atgaaagata tataacggag caaatatata attgtccagt
     9361 gtttgttaca gattatccaa aagatataaa agctttctat atgagaatga atgaagatgg
     9421 taaaactgtt agagcaatgg atttactagt tcctggagtt ggagaaatcg taggaggttc
     9481 tcaaagagaa gaaagagaag atgtattgtt agatagaatt aatgaaatgg gattaagaga
     9541 agaagattat tggtggtatt tagaacttag aaagtttggt actgctactc actctggatt
     9601 tggtcttgga tttgaaagaa taattatgta tatgacaggt atgtctaaca taagagacgt
     9661 tatacctttc ccaagaactc ctaaaaatgc tgaattttaa taattatata taataaaact
     9721 tatataactt taagtataaa cactaaaatg acttgcatat ttatctattt tagagatatg
     9781 caagttattt ttattgtact ataaatatat aatttgatta cttttatttt gcgatataat
     9841 acttataggt ttatgtaata gtatgtaacc ttttaataaa atgcaacaca attacgtcct
     9901 tgttcttttg cagtatatag ggccttatca caattactta taaactcatt taatgttatt
     9961 gtctttccaa ttttctttgt ataaactcca atactaacag ttacacattt taaaggaaca
    10021 tcatcacgct caaaattgca ggactctata atttttcgta aacattctga tttaagttta
    10081 gcatctgcct cattacagtt aaaaatacat ataaaaaatt cctcaccacc atatcgtata
    10141 gcatgttcat tttcagaact tgtaaaatct aaaattactt tggcaatatt ttgtagacaa
    10201 tagtctccct tttgatgtcc ataattatca ttaaattgtt taaaataatc tatatctatc
    10261 atcattccag ttataggttc ttgatttgca tggctgatta attttttttg tagaatatct
    10321 tcaaggtacc tacggttgtt cattttagta aggtcatctg tcataacttt gtcgttaagt
    10381 aaattattta tatgagatat ttcttcatat tgttgactaa ttaaaagttt atcatagtaa
    10441 gtaaatacac gatttctata tagcattgta gaaacaaaaa tggcaaggga tgttataaaa
    10501 aatgtgttga caatattact aaactcattg cctggtatag tttgaatccc aggtaagaat
    10561 aaattaagaa acaaaaagca tcctccaaaa actattactg attctccaaa ttttaatact
    10621 gagaaagctg cagctgctaa cataatataa ctaaatacac ttaggtcatt tccacctaac
    10681 tggtcaagta gagtaattac acaaccccat aggcagaaaa aagtcacata aattatccat
    10741 aaatttatat aaaacgtaca gcaatccttt tttttattcc aaaggtaaga aaatacaaac
    10801 ataaagacag aagatacaca aaatagtgtt acatacatat aaaaatacca ttggcgtctt
    10861 ggtgtgacaa aagggcctcc ttctctagct gagattgata tcatcataat tatttgagat
    10921 attactatta ttatgcaaat gattttaccc gtatgataaa gataattgca gagttctttc
    10981 tcaacattgt ctttgtgttt tttgtctaga ggaaatccaa aaaatttttt tattttttct
    11041 aagttcatat aagcctccat taaaaataaa gcataaaaat aagtattcct ttctatcatt
    11101 agatatttgg aatacttcgt ttatgattta attatatcaa aatatattgt gtgtaacaat
    11161 ctaaaattgt gatagataaa atttattaga taatgtcgag taactttatt gattaatact
    11221 ttattataat taagatttac agatagttta ctactgaaaa taaagaatag aataaattta
    11281 aattaagcat aataaaatat aaataataaa gttcgcatta acaaactatt taaatatcaa
    11341 gacctgttaa aaaacaacag caataaacaa ataaaataga taaatatatc caattaaggt
    11401 ataattaagt tttatatgat atagttatat ttgataattt acaatagtaa aacttggagg
    11461 gaaaaaatgg gtgaaaagaa aataaataat atgaattatt cacataataa ttattccaaa
    11521 aagacaggtg aaccaactag aaaaataaaa aatagccata tcactcttaa aagtaaagaa
    11581 gaaaaaattt gttctagttg caataaagaa aataatacta aggataatta ctgtaaattt
    11641 tgtggaaatg aactttatga aatagcatca ttaagaccac ttgaaacaaa actagacttg
    11701 aaatctaaaa taaaagagct ttcatatcat gcaaataaaa gaggtgtatt tttaacaaca
    11761 tttacaacca tatttatatt gtttattatt gcattgatat ttaaagctat tataactatt
    11821 caatttaatg atattagtta tttaataaat ccagcacata taatcctagc tctcaacttg
    11881 ggtcaaatta gtgtatctat gtctaccatg atgggttctg ggtttataaa tgccaatata
    11941 ggtttgttga tattacttat aatccctatt ttggtattat taatatcaaa cctaattttt
    12001 atgagaaaaa gatgtagaga ttcaaaaact gtattagcaa attctctagg agttggaatt
    12061 ttttatggat tagttttagc tattttatct atttttacta atgtaaaaac tagttcacat
    12121 agtatgttag aatatggata tgcattagaa tatagttatg aattttttag tgttctttta
    12181 aatggatttg tattagggtt tatatgtaca tacataacta cttataaaaa aagttatgaa
    12241 aaggaaaata tgtatttatc tcttttttca aattctataa gaacattcat attaggttat
    12301 gttttagtat tagtaatact attggtacta actatatctg atagtagtta cttaaatgaa
    12361 ctagatatga gtagttattc aaatggatta aatttattta ctatactccc acaaatagct
    12421 agttatatgt gggcttttgc aaatggaatt tcagtaacca taataaactc aacagtatca
    12481 atgtttactt taagttcttc atctttattt ggagatacta aattaatgtt ttatgcaatg
    12541 ggagcattgt ctatgttaat attattatta aatggataca agctaagatt taagtataat
    12601 acagatagta taagacctat aatagtattt agtatatatt atgcattttt gatgggaata
    12661 ttggctttat ttagtacatt tatactagat agtaatatta atttctttaa tacaactaat
    12721 tatggaacaa ctttaataat gcaatttaaa gtcttacaag ctattgtaat ttcatttgta
    12781 tatagttttg tgatatcttt aataggatat aagctaaatt cagctgatta aatatgattg
    12841 gagggaattt ctgtggaagg aaatactaat aataaagtag atgataaatt ttctaaggtt
    12901 aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa atcaaaatta
    12961 aatagagaga ttgatgatat tcaagaaaag aaagcaaaga tattcttgga aatgggaata
    13021 ctgacatatc aaaaaattag agaagattct attaatgata atagttttga taaattctgt
    13081 gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat taatgaaatg
    13141 gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt aaatgataaa
    13201 tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga ttttataatt
    13261 tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg ctgtggaaga
    13321 aaaattataa tggatttaga ataatattgg gaaacctatg tatagtatac atgggttttt
    13381 ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa ataaacacca
    13441 aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata aaataaatca
    13501 gtaaaaatat agatgcgaga aaaaaatatg aaaaaatatt gcaaagaaaa agttttcatg
    13561 ttattattaa tacataaatc aagaaaataa atttcagaaa cgaggtaatt taaaaaatgc
    13621 tagataaggt aaagggaaga ttaatagtat catgccaagc tttagaagat gaacctcttc
    13681 atagtccttt tataatgggg agaatggcta aggctgctat ggaaggtgga gctgttggaa
    13741 ttagagctca aggagttgaa gatattattg aaataaaaaa agttactgga cttcctgtta
    13801 taggtattat aaagagaaac tatgaagatt cagatatata tataacgcca actaaaaaag
    13861 aagttgatga acttttaaca acagggtgtg aaatgatagc attagatgct accaatagag
    13921 ttagaccaaa taatgaagac ttaaaagaac ttataaagta tataaaagaa aacggtgttc
    13981 tagtaatggc agatatttca aattatgatg aggctatcaa agcacaagag tacggtgtag
    14041 attgcgtttc aactacacta tctggatata ctccatatac taaaacatta gaaggtccag
    14101 attttgtatt aatggaaaga ttagttaaag atttagaaat acctgtaata gctgaaggaa
    14161 aggtaaatac tcctcaagat ttaaaaaagg tctttgagtt gggtgttcat tcatcagtag
    14221 taggttcagc aataacaaga ccacaattaa taacagaaaa atttgttaaa gcaattgaaa
    14281 ttaattaaaa attaatttat aaattaaaaa attattatat ttaactatat ttgaaaatta
    14341 aaattggagg taaagatatg agaacaacag atatgaaggg aatttattca gcattattag
    14401 tttcatttga taaggaagga aatataaatg aaaaagggtt aagacaaata attagacaca
    14461 atatagatgt ttgtaaagta gatggtcttt atgtaggagg aagtacaggg gaaaacttca
    14521 tgctttcaac tgatgaaaag aaaagaatat ttgaaatagc taaagatgaa gtgaaagaag
    14581 aaataaaatt aatagctcaa gttggttcag ttaatttaaa agaagctgta gaacttgcta
    14641 aatttacaac tgatttagga tatgatgcaa taagtgctgt tacacctttt tactataaat
    14701 ttgattttga agaaataaaa cactattata atacaataat aaattctgta gataatagat
    14761 taataatata ctctatacca tttttaactg gtgttgatat gagtttagac cagtttggag
    14821 aattatttga aaatgaaaaa ataataggtg ttaagtttac agcagcagat ttctacttat
    14881 tagagagaat gagaaaaaca ttcccaaata aattaatatt tgcaggattt gatgaaatga
    14941 tgttaccagc tacagtttta ggtgtagatg gggcaattgg ttctacattc aatgtaaacg
    15001 gtgtcagagc tagacaaata ttcgaattaa ctaagaatga aaaaatatca gaagcacttg
    15061 aagtacaaca tgtgactaat gatttaataa cagatatatt aggaaatggc ttatatcaaa
    15121 caattaaatt actacttgaa gaacaaggtg ttgaggctgg atactgtaga caacctatga
    15181 aagaagcaac tgatgaaatg aaatcaagag caaaagaaat atacagaaag tatttttaat
    15241 aaaatacttt tagtatggga ggattagtca aatggtagga tttacttgga tagattttgc
    15301 aatactagtg gtttacttat tggcagtttt atttgcagga ttgttatttt caaaaaagga
    15361 aatgaaggga aaagagttct tcaaaggtga tggaactatt ccgtggtggg ttacatcagt
    15421 atcaatattt gccacattat taagtccaat atcattttta tcattagcag gaaattctta
    15481 tggtggtact tggattttgt ggtttgcaca attaggtatg tttatagcaa tacctttgac
    15541 aataaaattt ttcttacctc tatatagtag gttagaaata gatacagcat atgaatactt
    15601 agaaatgaga tacgaaagca aaggtcttag agtacttgga gctttgatgt ttataatata
    15661 tcaaatagga cgtatgtcta tcataatgta tcttccatca atagttctgg ctggtcttac
    15721 aggaatacct gtaaatgtac tgataatagt tatgggtgta atagcaataa tttactcata
    15781 tacaggtgga ttaaaggctg tcttgtggac cgattttata caaggaatgg tattaatagt
    15841 aggtgttaca gggacattgt tctatttaat agcaagtata aatggtggtt ttggaacagt
    15901 aatggatact cttacaactg gacacaagtt cttagcaagt aatgaagtta tgtttgataa
    15961 aaatatacta tctacaagtg catttataat atttgttgga gctggtctta atactttttc
    16021 atcatatgta tcgagtcaag acatagttca aagatttact acaacaactg atataaagca
    16081 acttaataaa atgacatatg gaaatggtgt tttatcaatg ggacttgcaa cagtatttta
    16141 tctaattgga acttgtttat ttatatacta tactcaaaac ccagatttag ctcaaacagt
    16201 tcaacaagac caagtatttg catcatatat agcttatgaa ttaccagtgg gtataacagg
    16261 aatactactt gcagcgatat atgcagcttc tcaatcaact ttatcgacag ggcttaattc
    16321 agttgctaca agttggactt tagatataca aactatcata acaaaagata taagtatgga
    16381 aaagcaaact aagatagcac aatatatatc attaggtgta ggtatacttt ctatagctgt
    16441 tgcaatagta ttagcaaatg gagaaataaa gtcagcatat gaatggttta atagttttat
    16501 ggggctagta ctaggtgttt tagcaggaat atttgtactt ggtgcatttt gtaaaaaagc
    16561 gactaaaatg ggagcatatg ttggattcat agtatcagca attttagttg tttatttaaa
    16621 ataccgtatg ccagaagtaa cttcttgggc atattctcta ataacaatca cgacttctgt
    16681 agtggtaggt acaatagtta gtgcaataga agctaaagtt aaagcaaaag tatctttacc
    16741 aaaagctgag actactgttt attatgaaaa ataatattta aatgataaat tgtcttatag
    16801 agtgattaga taaataattt agttactcta taagattgta taatatactt tgtatataaa
    16861 agtataaaaa tagactttat aattatatag ggagatagtg cttatgatat ttggaaatat
    16921 aaatcatagt aagacatatt caacattgca tgaagattta cttagatgtt ttgaatatgc
    16981 taaagataat tcattaatag attatgaaaa aggtacttat acaatagatg gtgatgatat
    17041 ttttgtgaat attgtagagt ataaaacttg tgaaaaagaa gatagattct gggaagctca
    17101 taagaagtat atagatgttc atttgatgct tgatggatgt gaacgaatag atataaattt
    17161 tatagaaaat ttagaacaaa aaatatatga agaaaagggc gatttcttgc ctcttgatgg
    17221 taaaaataat ggatacatgg aacttagaaa aggtgatttt ttagtttgtt atccggaaga
    17281 tgctcatatg acaggaataa aagttttaga aaaagaaaat atcaaaaaag ctatttttaa
    17341 ggttttggtt agataatgat aatttatata accactaggg ctgaaaatag tgatttaact
    17401 ttcagcgatt agtgagactt cgtaaatttt agttagatta ctattttcag ccttagtttt
    17461 ttatgagaaa attgaaaata attaaaagct taaaattttg taaaggagtt aacttatgga
    17521 aaatatagat gtattcttat taataggaca aagcaatgct agaggattgg gaaatccaaa
    17581 agagagcatc attccaaatg aaaattgttt tgaatatttg agtactgatg aaattataaa
    17641 tatgagatgt gagctagaga cttcagaagg tgatggtact atagcacctg cattttcaaa
    17701 tgaatggaat aagcttactg gaaataaagt ttgttttatt cacaatgcaa aagatggttc
    17761 aagaataaaa aactggaatc atgataataa ctggtttcta aatgacacta tagaaaaatt
    17821 taatacaggc tgtactacct taagtaaaca ttataatata gaaaataaat acgttatttg
    17881 gattcaaggt gaaagtgatg caaaatatgg tagtgatgta ttgtactata aagaaagtct
    17941 aaaaaaaatt gcgtataggc ttaaagaaga atgtatgatt gataaaatgt ttgtaagttt
    18001 aactggttat tggcttggag aagatgaata ttttattaga acaagacgaa ttgcagcagc
    18061 acaggagtcg gcttgtagtg aatgtgatat attatgtgta ggaagcaaaa ttgctatgca
    18121 gtttcatgac agaggattaa ctattgatga tgttcattac actcaagaag gtcttaatat
    18181 gttaggagag gacttatgta aaaatattta taagtatcat ataaccaaag aaaaaagtgt
    18241 attaagtgat actatagact tatcagaagc aaggaaatat atctgtgaat tggaaaagat
    18301 aagtaaaaga tttgtgtaat ataaaatttt aaatagagtt gttaatttat aaaaataatt
    18361 tgtatggaga tatgttatga aaaattacgt ttgtatagat gttgggggta cttcaataaa
    18421 atatggtttt attagagaag atggatttat aattgataaa tcaagtcttg atactgaagc
    18481 aaaggaaaaa ggtggagaag gaatattagc caaaataaaa gatatagtaa aaaaatatat
    18541 agaggaaaat gaaataagtg gtatatgtat atcaactgca ggtatggttg acccagttga
    18601 aggtaaaata ctatttgcat tagaagaatt aattccaaac tataagggca tgcaattaaa
    18661 aaaagaagta gaaaaagagt ttaatataaa atgtgaagtt gaaaatgatg ttaattgtgc
    18721 tggacttggt gaaatgtggc ttggagcagg taaagatgca acatcttcta tatgtttaac
    18781 aataggtact ggaattggtg gatgtattat aataaataat aaactgataa atgggtttag
    18841 taatagtgct ggtgaagtag gatatatgaa tgtaaatgga agttcttttc aagaattggc
    18901 atctactagc agtctaatta aaaaagtagc aaaaataaaa aatttgaatg aaaatgactt
    18961 aaatggaaaa ataatttttg atttagcaaa gaataatgat gaagattgtt taaaagaact
    19021 cgataatatg ataaaatcat tagcagttgg aatcgcaaat atatgttatg taataaatcc
    19081 agaagtggta attttaggtg gtgggattat ggcacaagaa aagttcctaa aaccaaaaat
    19141 agacaaagca ttaaaggagg ttcttataga gagagtttac aaaaacacta atatagaatt
    19201 tgcaaagaga caaaatgatg caggaatgct tggagcatta tataattttt taaacaaaat
    19261 ataaagaata gaagggtttt taatgagtat tttagaacaa ttggaatcac caacttttaa
    19321 agtcacaaaa tcagataaat tattaatagc ttatataaaa gaaaatatag atgatgtatt
    19381 ttataaacca atatcacaaa tagctaaaga gagcaatatt ggtgaagcaa ctataactag
    19441 gtttgtaaaa aaaatgaatt ttaatggatt acaggatttt aaagttactt tagcacaaga
    19501 aatatcaact atcaataaaa aaaatattat aaataaaaat atacaaaatg atgagccagc
    19561 tttggataca gcaaaaaaac ttttaagttc aaatgtaacc actttagaga acacagtaga
    19621 gataatcaat agtaaggatg tacatgactg cgcaaggctt ataataaatg caaagaaagt
    19681 ttacttcata ggtattggat attctggtat aatagcacag gattcaaact ataaatttat
    19741 gagaataggt cttaattgtg taagttttga cagtagtcat acaatgataa tgatgtcttc
    19801 cataatggaa gaaggagatt taataattgc aatttcacat tctggtgaaa cagaagagat
    19861 tataaaaact gtaaagcttg caagagccaa taatgctaaa attatatcaa ttactgaaaa
    19921 taaaaattct gaattaaagg atatttcaga tgtacactta tcttatgtat cgggagaaac
    19981 agtactggaa actggttcaa tatcttctaa attagcacaa tttttcatta ttgatttagt
    20041 atatacacag gtagtaaaag aactttctaa tgaagctatt gaaaggaaaa ttaaaactac
    20101 aaatgctata aaattgttta aaaatgaata aaataagaaa tttgtaatgt tttatagagc
    20161 tctcttaaaa atttaggaat ggagagatat tacatgtcta aattaaaaga ttataatatt
    20221 gataaagtaa taatttttaa taatttaagt actaaaacga agaatcaact taaagaaaaa
    20281 gttaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga tagcttagac
    20341 agtgtttata tacttttaga aggaaaggta tctattttta agataagtga aaatggagag
    20401 agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac acttgacgct
    20461 tctaaaaact ctactgttgg ctgtgaagca tttgaaaatt caattgtagc ttattatagt
    20521 attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga tattatttct
    20581 tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat atccataaaa
    20641 gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg agtttgctgt
    20701 ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga tatgcttggc
    20761 tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga acttattaaa
    20821 attgacaata aaggttttta tgttaaagaa actgaattat caaggtattt taaaaataat
    20881 taattatgat taagtaagag tattattaga attttaatta aataaaaata ttat
//
LOCUS       DAMJYC010000066        20712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJYC010000066 DAMJYC010000000
VERSION     DAMJYC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.067"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            161..3109
                     /locus_tag="PC472_002645"
     CDS             161..3109
                     /locus_tag="PC472_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2727962.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3087..3551
                     /locus_tag="PC472_002646"
     CDS             3087..3551
                     /locus_tag="PC472_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2727963.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3631..3933
                     /locus_tag="PC472_002647"
     CDS             3631..3933
                     /locus_tag="PC472_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2727964.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3995..5359
                     /locus_tag="PC472_002648"
     CDS             3995..5359
                     /locus_tag="PC472_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2727965.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5394..5867
                     /locus_tag="PC472_002649"
     CDS             5394..5867
                     /locus_tag="PC472_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727966.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            6039..6689
                     /locus_tag="PC472_002650"
     CDS             6039..6689
                     /locus_tag="PC472_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2727967.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREGINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6863..7795
                     /locus_tag="PC472_002651"
     CDS             6863..7795
                     /locus_tag="PC472_002651"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2727968.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYYDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKNRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8069..8782
                     /gene="ispD"
                     /locus_tag="PC472_002652"
     CDS             8069..8782
                     /gene="ispD"
                     /locus_tag="PC472_002652"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2727969.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8779..9264
                     /locus_tag="PC472_002653"
     CDS             8779..9264
                     /locus_tag="PC472_002653"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2727970.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9359..9567
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9631..11346
                     /locus_tag="PC472_002654"
     CDS             9631..11346
                     /locus_tag="PC472_002654"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2727971.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11468..12913
                     /locus_tag="PC472_002655"
     CDS             11468..12913
                     /locus_tag="PC472_002655"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2727972.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13351..14832
                     /locus_tag="PC472_002656"
     CDS             13351..14832
                     /locus_tag="PC472_002656"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2727973.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15053..15208
                     /locus_tag="PC472_002657"
     CDS             15053..15208
                     /locus_tag="PC472_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727974.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15353..16750
                     /locus_tag="PC472_002658"
     CDS             15353..16750
                     /locus_tag="PC472_002658"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2727975.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLGMG
                     IVLEDTRQGVKWKRI"
     gene            16735..17148
                     /locus_tag="PC472_002659"
     CDS             16735..17148
                     /locus_tag="PC472_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2727976.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17254..18012
                     /locus_tag="PC472_002660"
     CDS             17254..18012
                     /locus_tag="PC472_002660"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2727977.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            18025..18762
                     /gene="rlmB"
                     /locus_tag="PC472_002661"
     CDS             18025..18762
                     /gene="rlmB"
                     /locus_tag="PC472_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2727978.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18765..19292
                     /locus_tag="PC472_002662"
     CDS             18765..19292
                     /locus_tag="PC472_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2727979.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19357..20004
                     /gene="sigH"
                     /locus_tag="PC472_002663"
     CDS             19357..20004
                     /gene="sigH"
                     /locus_tag="PC472_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2727980.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20078..>20712
                     /locus_tag="PC472_002664"
     CDS             20078..>20712
                     /locus_tag="PC472_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2727981.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKP"
BASE COUNT         8569 a         2163 c         3881 g         6099 t
ORIGIN      
        1 aaagtattat ttgtaaaata taaaaaaaaa tttcttaata tttgaataaa agaatagctt
       61 aatttaaatg ttattacttt ataaaaaaat aataagtaca aaaaaaacaa tatatggcat
      121 ataagttgct atataaataa taaggaggga acaattataa atgaggcaaa gaataaaaaa
      181 cttaattgaa aatgaagata agaaaaatcc tttaacagat gaagcgattt caagtaggtt
      241 aaatattcga agagaagatg ttaccttttt gagaaatgag ttaaaaatag aagattcaag
      301 acaaagaaga aaagttgttt taattaaagc tataaaagat attttaaaag aagaaaaaac
      361 tataaataaa aatgatataa caagaagatt aaacagcatt gggtttacag tatcaagatt
      421 tacagtcata caatacttaa aagagattca agattctggt gacctaaaac ttgataaaga
      481 gagtgatgat aaaaaagaag aaataatcaa taatagagaa aataaagata tagcatttga
      541 taaactcata ggtagtagtg gtagcttaag tacaattata aaactagcca aagcagcaat
      601 tctttatcca cctcatggtc ttcatactat tattatgggg ccaactggtg ttggaaagag
      661 tgagttagca gagtgtatgt ataaatttgc agtagaatca tccagattcc ctaagaatgc
      721 accatttata gtttttaatg cagcagatta ttcagagaat ccacagcttt tgctatcaca
      781 gttatttgga cacgttaaag gtgcttttac aggagctgat gagaacaaag aaggattagt
      841 atcaaaggca gatggaggaa tattgtttat agatgaaata catagattac ctcatgaagg
      901 tcaagaaata ctatttcaac tgattgataa aggtatggtc agaaagttag gagaaacaag
      961 actaacacat aaaatagatg taatgattat ttcagcaact tctgagccaa tagattctca
     1021 tttacttaat acatttaaaa gacgtatacc tgtaacaatt gaaataccag aattagttgc
     1081 aagaccatta aatgagaggt ttgacattat aaataatttc ttcttaatag aagctcaaag
     1141 aatgggagca aacatacata taaagtcaga tgtattaaaa gctcttatgc tttacgattg
     1201 tataggtaat gtaggtcaac ttagaagtga tattcaagta gcttgtgcta ggggactatt
     1261 aaaccaactt acaaacaaac taaaagaagt aaatataagc atatcagatg tacctggata
     1321 tgtaaaacaa ggtcttataa aaatcagaaa ttgtagaggg aaaatagaaa attatgtaga
     1381 tggggattta attatagact caaatcttca aggtgagata gaaaagaaaa atgaagatat
     1441 atatactttt ccagatgaaa tatacaaact tactgaaaga agacatattg atttattgga
     1501 acaaggttta gattttgatg ttataaatag aatcattgga ggagaattag aaagttgcat
     1561 tcaaaaatac ataaaacaag ttaaaaaagt ttctattagt aacaatcttc cagatataag
     1621 tccaattgtt ggaaaatata tcgtagagct aacttatgaa gtaatcaaaa tagcatcaca
     1681 atacctcagt tcactagaac cgaatctaca tttatgttta gcagttcacc taaaagcagc
     1741 ttttgagaga ttaaaagaag gaaaagtaat atcaaatgct catttgaaag aaataatagg
     1801 aaattatcca gtagaatatt ctgtagcaga aaaggtagct caatatttaa aggaaagata
     1861 taacataaac ttaccaaaag aagaaatagg tataatagca atatatttta aaatgagtag
     1921 tagaagagat atgtctgaac aaaaaaagat aggcgtactt cttatggctc acggaagagt
     1981 agcatcttct gtatgtgagg ttacaaataa actattagga attaatcacg caaggtattt
     2041 agatatgcca tttgagaaga agccggaaga aatgattgaa gaagctacta caataataaa
     2101 agatattgat gaaggtagag gtgtgattat attagtagat atgggttcac tttcatattt
     2161 tggagatatt atttctggaa agactggaat taatataagg acaatatcta gatttgatac
     2221 attgctttct atagaggtta tacgaagagc agttttacca gatttaacaa tagacgagat
     2281 agttgatgaa gttaaaggta tagagccaca agtaaaagaa aaggttagta aatgtaacaa
     2341 tacaaaagta gattttagaa aacctataat tttaacatta tgtattactg gattaggggg
     2401 agcttgtaag ataaaaaaaa tcttagagga aaatattaaa agtataactg aaaaggttga
     2461 tatagaacct ataggtatga ttgaatatcc aagtcttgat ttagagataa aacaaataag
     2521 caatgataaa aatgtaatcg ccatagttgg aacaattgac cctcagatag caaatatccc
     2581 tttttttagt ctagtagaca taaatgataa agcaaagtta aatcaaataa ataacctaat
     2641 tgatacaaat caaattggtg aaacaaataa aaaatatgaa ttatcagatt taatacataa
     2701 agaagatata tttgtggatt tacaggttaa atcaaaagaa gaagttctaa aatatatgag
     2761 taaaaaactt agagagaaag gttatgtgac aaaaaatttc tataaaaaag tagtggaaag
     2821 agataactta tttccaactg aattatcaga tgaaatagca atacctcaca cagatggaat
     2881 agatgtaatt aggccagcta taagtatagc catcttaaag aagcctattg tgtggagtaa
     2941 aagtaaagta aaaataatac ttttattagc tgtagaccaa aagtgtttta atccattaag
     3001 cactctactc tcatttatag aaacagatga atttaagaag ataaaaaaca ttaaagatag
     3061 agattttatt agggagatga tattagatgg agtcaatgaa aataattagt agcaatttaa
     3121 tttttaaaaa tattgaagtg tctaacaatg aagatgcatt aaagtttttg ggacaacgtt
     3181 tgtttgacga acagtatgta aaagaaagct acatacaagc tgttgtagca agagaaaaaa
     3241 agtatgctac agggttacca acagaaatat acggagtggc aataccacat acagatatag
     3301 tacatgtaaa tgagccagga atagcaattg gaatactgaa taaacctgta aaatttataa
     3361 tgatgggtac agacgatact gaaattgatg taaaagttgt atttatgtta gcagtgaaag
     3421 agccacaaga acaattgcaa ctattagaaa aacttatgac tatatttcaa gataagaata
     3481 tactaaataa tattgttgac ttaagtgaag aaagtgtgag tgatttatta aactctaaat
     3541 tgaaaaatta aacatgtaaa gtatattaaa aatgtaaaaa gattattaaa gatttctaac
     3601 tatagatatc tttaaaataa aataatttca atgggggtga ctaatgtggc taaaaagata
     3661 ttagttgcat gtggaacagg agtatgtact tcaacaattg ctataaacaa attaaagaaa
     3721 gcacttcaag acattggtaa attggatatg gttaatatta cacaatgtaa agtagtagaa
     3781 gttgcatcaa aagcacctga ttatgattta ataatttgta ctacacaagt atctagtagt
     3841 ataaaaacac cagtgataaa tggcttacct tttttaactg gtgtcggaat ggataaatta
     3901 atcaatgatg tattagaaga gttagaatta taattaaaaa aattaaggaa catggtctct
     3961 aaaatcataa gttttagaga cttaaaatta aataatggag ggtctttata tggagatatt
     4021 aaatttcatt gttggtctag gtgcacaagt aatgatgcca ataataatat gtatttttgg
     4081 tttgatatta ggtacaaaac taggaaaatc tttaagagct ggtttaactg tgggagttgg
     4141 gtttataggt cttaacacta ttatagcttt gctaacagat aatttaggtc cagctacaca
     4201 acaaatggtt aagaatttag ggctaagttt atcaataata gatgtaggat ggccagcagc
     4261 atcagctata gcttttgcat caacagtagg ggctttaatt attccaatag gtcttgtggt
     4321 aaatatagtt atgctaatta ctaatactac tcaaactgtt gatgtagaca tatgggatta
     4381 ctggcatttt gcttttacag gagctttagt ggcaggggct actcaaagtg taatgtgggg
     4441 agtatttgca gcagtagcaa atatggtaat tgtattggta atggcagatt taactgctcc
     4501 aggtatagag gaatatttag gtatgccagg tatatctctt cctcatggat ttacacaggc
     4561 atttgttcca attgctatag tagtaaataa attattagac ctaataccag gcataaataa
     4621 gattgagata aatgctgata cattgcagaa aaaatttgga ttatttggag aacctttaat
     4681 aatggggtct gtaataggtg taataattgg tattgctgcg aaatatgata taaaaggaat
     4741 attacaatta ggtgtaacta tgggagctgt attaatatta attccaaaga tggcagcatt
     4801 attaatggaa ggattattac cagtatcaga agctgcacag gaatttattg agaagagatt
     4861 taaaaacaga ggcaagattt atataggctt agattcagca gtaggaatag gtcatcctgt
     4921 tacattatca gttgcactag tgttggttcc tttgactata cttatagcag ctatattgcc
     4981 aggaaataaa gttttaccat ttgcagattt agcagtaatt ccatttgcac ttgtattaat
     5041 cgtaccaatt acaaagggaa atgtgtttag aacattaata ataggaataa ttataattac
     5101 ttctggatta ttaatcgcaa ctaacttagc tccattattt acccaaatgg ctttaaatgc
     5161 tagttttaaa atgcctgaag gagcaactat gatttcaagt atttgtgatg gtgcgaatcc
     5221 attaagttgg gtatttgtaa aagttatgaa ctataaagtt attggaggag tagtttttgg
     5281 agttatagct ttaggaatgg ctatttacaa taggaataga ataataaatg aaaataaaaa
     5341 gctatctata gaagaataag gctaaaacaa ttttattaag gagtgtatag gttatgaaaa
     5401 aatataaata tgattctaaa agatacttac tcagtgttac aataccagga ttttttatga
     5461 ttttaatatt gatttatgca ctgtttaata actatacaaa ttttaatttg aatatatata
     5521 gtttgttaat aattgtctgt acttatggta tattcaatac ttttatatca tcaagtaatc
     5581 ctaaaaaaat tattattgat agcaaatgta tacattttac ctcatttatg actacacata
     5641 agtatgaaat taaagatata gaaaagataa gaataagaga attttataat aaaaaaattt
     5701 atgtaaaaat taatgatgga aaccttttta aaggtaggta ttggataaga acaaatatgt
     5761 ttaataatag tatagcatta tatagctatt tcattgagtt ggaagagtgc ttatatccag
     5821 attcactaaa atttagaaat aaaaaatttg agaataagaa catataaaaa taaaaaattg
     5881 aattgaagcc tagcttaatc ttttttgata agatataagt ttgaacttta tactaaattt
     5941 ttagaatttt caatcaagac aataacataa tagctaagca attagaacac aattaagatg
     6001 tataaggcat gtatatgatg aatgggagga tttaattaat gttattagag aaattaagaa
     6061 aagaagtatt acaagcatct ttagaccttt taaactataa tttagtgaca ttaacaggag
     6121 gaaatgtaag tgggagagat gaacaaactg gctatatagc aatcacccca agtggaatgg
     6181 attatagaaa tcttacacca agtgatattg ttatagtaga tgtagatgga aatattatag
     6241 atggaaaatg gaaagcatca gtggatttat cagaccactt gtatatatat aagcatagag
     6301 aaggcataaa tagtataatt catacacatt ctacatactc aagttgtttt gctattttaa
     6361 atgaacctat agaatgtgca agtacaactc ttgcaaatga agtagggggt tcagtaccag
     6421 ttgcaaaatt ttcaccacca acatctaaaa agatgggaaa atgtgttatt gaagcaattg
     6481 gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat tgcagttggt ccatctgtag
     6541 gacatgctct tacagccgct gtaatgttag aagattcagc aaaagtatat tacttagcaa
     6601 aatcaattgg cacaccagtg ctactgcctg atgaagaaat tcaaagagcg agagatgtat
     6661 tttttaatgt ctatggtcag gataaataaa ttagataaaa ttttaaaagt aatatgaaaa
     6721 tatagatatt caatgatatt aaggagattt ataatgatat aataagttta atagttaaga
     6781 agtaaaatat gaaatgtaat agtatataaa ttcatttaag actaaaaaga ataagataaa
     6841 ataaaaagga gtctgtattt ctatgtatta cttattaaac aaaagcacaa atccttactt
     6901 taatttggca ttagaagaat atctattttt aaatgataaa tataatgatg atattataat
     6961 catatggaga aatgaagaat ccatttttat aggtaaaaat caaaatccat atcaggaagt
     7021 atattatgat gttattgaaa agggagaaat tcctatttta agaagaatat ctggaggagg
     7081 aactgtttat catgacttag gtaatatcaa tatgtcattc atacaaaaaa ataggcaact
     7141 tcatgagata gattttttgg agcacactaa gtttatgcaa aagatgcttt caaccttagg
     7201 actagatgta tctataactg aaagaaaaga tttattttta aatgggaaaa aaatttcagg
     7261 ttctgcacag tctataaaaa gaaaaaattc tctttatcat ggaactcttc tttacgattc
     7321 agatttaaat aaattaacta aatatttaaa ttcaaataaa gcaactgaat caaatgcaac
     7381 taaatctgtt tcaagtaaag ttacaaatat taagttttta ctcgaaaaag atataaatta
     7441 ctttttagat tattgtgttg aatatttaaa atctaatata aagaatataa aagaacttga
     7501 attggatgaa gaagatatta aaagtgtata caatatagga gaagataaat ataaaaagat
     7561 agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg ctaccattta ataatgaaag
     7621 taaaataaat atacatgtta acagatggaa agtatcaaaa ttttttattt cttatgatga
     7681 taatgtaatt aatatggaca agttgttaga tttgaatttc tttaaagaag aaataaaaga
     7741 gagtatttta gaaaattatc cacattacat agatttaatc aatttaatat tttaatataa
     7801 aacctctaat ttgataaaat tgtcttatat caaactagag gtttttaata ttcttgcata
     7861 ttaacaacca ttacaaaatt gtaaaattaa aaacaacact attgaaaaat caatatatat
     7921 tgtaataaat atatgtgttt ttcattagca aatagacatt aactatttat acaactgaaa
     7981 atttaatata taattaatca gatggttaaa ataaaaatat taaaagtttt aataaatgat
     8041 ggttaaaaat aaaatacgag gtgataccat gtatagtgtt ataattgttg cagcgggaag
     8101 cggaaggaga atgaacttag atataaacaa acagttcata aaattaagag aaaaagaaat
     8161 tattgctcat acaattcaag ttttttatga aaatataaat atagatgaaa tagtggtatg
     8221 tataaaaaaa gaagaagaag atttttttaa agaaaatata ataaataagt ataactttaa
     8281 aaatataaaa atagcatatg gcggaaaaga aagacaggac tcaatatata atggtttaaa
     8341 aaaattagat gaaaattgtg atatagtact tatccatgat ggagcaagac catttgtcga
     8401 ccatagaata atcaatgaat ctataaaggt agctaaagaa aaaaaggctg tggttgtagg
     8461 tgttccagta agtgatacta taaagatagt gtcagatgga acagtacaag aaactccaga
     8521 aagaaatttg ctgtgggcag cacaaacccc tcaaaccttt gaatataatc tcataataga
     8581 tgcttatgaa caagcatata agaacaatta ttatggtact gatgattcaa tgttggtaga
     8641 aaatataggt caaagtgtca ctatggtaat gggttcttat gaaaacataa aaataactag
     8701 tccagaagat ttaaatatag cagaacaaat attaaatatg gaaaaaagag atgaagttaa
     8761 cagtagaagg agaattatat gagaatagga ttaggatatg atgtacacaa attaacagag
     8821 gatagaaaac ttataatcgg tggtgtagag ataccacacg ataaaggttt gttgggtcat
     8881 tctgatgctg atgtattgat acatgcaata atggattcta ttttaggtgc tctagcatta
     8941 ggagatatag ggaagcattt cccagataca gatgaagaat ataagggagc agatagtatg
     9001 aaattattag aacatgtgta taatctaata acaagtaaag gatataaaat tggtaatata
     9061 gacagtacta taattgctca aagccctaag atggctccat atattgagag tatgagaagt
     9121 aatatatcaa aagttttaaa cacagacatt gataatataa acataaaggc aacaacagaa
     9181 gaaggtcttg gttttacagg agcaaaacag ggaatagctt cacaaagtat ttgtttatta
     9241 ttgttgacaa gtcagaataa ttaatatatt attagtaata agtttaatgg atatcaagta
     9301 ataggcctat atagaatatc tataatgtat gaatttaagt gaaattaata taaatataaa
     9361 attagctatg ataaagaaat agtaaaatga tgaaatcttc acagagagaa aacggtggtg
     9421 agagttttca agaggaagtc tttgaacctg tcttttagct taaaggtata aaaatcttta
     9481 cggtaggaac cgttatattc ccaaaagaga gctattcata tagctaacta gagtggtacc
     9541 gcggaaatta aacctttcgt ctctatactt gtatagagat gagaggtttt ttatattttc
     9601 aggaagttaa tattcaggag gaatctatta atgaaaatgt caaaaatgtt tatgccgaca
     9661 ttaaaagaaa tcccagcaga tgctgagatt acaagccatc aattaatggt taggtcaggc
     9721 atgataaaaa aaatgacttc aggtgtttat aaccaattac caatggggtt aagagtgttc
     9781 aaaaaaattg aacaaataat tagagaagaa ttaaataaaa aagattgtca agaaatactt
     9841 tgtgcagcct tacttccttc tgaattatgg aaagagtctg gaaggtggac tgctatgggt
     9901 gaagaaatgt ttagactaaa agacagaaca gaaagagagt actgtcttgg gccaacacat
     9961 gaagaagcat tcactgatat aataagacaa gaaattactt catataaaca attgccacta
    10021 aacttgtacc aaatacaagt aaaatataga gacgagagaa gaccaagatt tggtgttatg
    10081 agaactaaga cttttacaat gaaggatgcc tatagttttg atgtagatga taaaggttta
    10141 gataagtcat atcaagatat gtttgatgca tatgtaagta tatttgatag atgtggattg
    10201 gagaatagcc ctgtacaggc agattcagga gctataggag gttctacatc agccgaattt
    10261 atggttaagt cagaagttgg tgaagatgaa gttgtatttt gtagtggatg tgattatgca
    10321 gccaatgtag aaagagcaga atcttgtaat ctagcatcac aaaaagaaga aatgaaagaa
    10381 ttagaagagg ttcatacacc aggagcagct acaataaaag agttagaaga atttttaaaa
    10441 acttctccag ataagtttgc taaaacttta gtatatgagg cagatggaaa aacagttgta
    10501 gtcgttgtaa gaggagatag agaagttaat gaaatcaaag tatctaatgc cataggttct
    10561 gttatagaat ttgctcttgc tacagatgat gtagtgagaa aagtaactaa tgcagaagta
    10621 ggatttgcag gtccaatagg tataaatgct gactatgtat ttatagataa agaaattgtt
    10681 gaacaaagaa acatagttgt tggggctaat aaaactgagt accatataaa aaatgctaac
    10741 tatggaagag attttgaagg aatagttgga gattttagaa atgttcaaga aggcgacaag
    10801 tgtatagtat gtggtaagcc acttgaaata gctagaggtg ttgaagtagg acatatattt
    10861 aaactaggaa ctaagtactc agaatctatg aatgcaaact tcatagacaa agatggaaaa
    10921 tcaaagccta tagtaatggg atgttatgga ataggtgttg aaagaacagc agcagctata
    10981 attgaacaac ataatgatga gaaaggtata atatggcctt tgtcagtagc tccataccat
    11041 gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa tatcaatagc tgaaaatata
    11101 tataatgatt tgcaagctat gggtgtagaa gttttattag atgacagaga tgaaagaata
    11161 ggtgtcaaat tcaatgattc agaattaata ggaataccta tgaggattac agtaggtaaa
    11221 aatattaatg aaggtaaagt agaatttaaa cttagacata aagaagataa agagataatt
    11281 gacatagaag aaataaatga aaaagtaaag gcagagttta tcaggaataa tgttaggtta
    11341 ggataataac ttaaataaga tattgtatag ataatcaaag ttgtacttta tagacaccat
    11401 tttatctata tataaggata taaaatggtg tttttttata aaacaaataa atgaggaggt
    11461 aattgaaatg gcaaaaaatg aaaaacaatt tgttgaagaa ataacaaaga tggaagatga
    11521 ttttccacaa tggtacacag atgtaataac taagactgac ttagttgatt atgcaccagt
    11581 aaaaggtttt atggttgtaa agccatatgg atatgcatta tgggaaaaga tgcaagaatt
    11641 tatggataaa aaatttaaag aaactggaca taaaaactgt tattttccat tattaatacc
    11701 agagagttta ttaaacaaag aagcagaaca cgttgaaggt tttgctccag aagttgcatg
    11761 ggtaacacat ggaggaaata aaaaattaga agaaagatta tgtgtaagac ctacttcaga
    11821 gactataata tgtacaatgt atgcgaaatg gttaaaatca tatagagaat taccatatct
    11881 ttataatcaa tggtgctcag ttgttagatg ggaaaaatca actagacctt tcttaagaac
    11941 atctgagttt ttatggcaag aaggtcatac attacatgaa acagcagaag aagcacaaga
    12001 agaaacaata caacaattag aagtatataa agcgctatgt gaagaattac ttgcaatgcc
    12061 agttgtagct ggtcaaaaaa gtgaaagtga gaaatttgca ggtggtgaaa gaacatatac
    12121 tatagaagct atgatgcatg atggtaaagc attacaatca ggtacaagtc acttcctagg
    12181 tcaacacttt actaaggctt ttgatattac ttttgcagat agggaaggaa atttagctaa
    12241 tccataccat acatcttggg gagcatctac aagacttata ggtggactta taatgactca
    12301 tagtgataac agaggtttag tattaccacc aagagtagct ccaattcaag ttgttatagt
    12361 tcctatagct gctaaaaaag gaaatgtaat ggagactgtt gataaaatat atgctgattt
    12421 aaaagctaag ggagtagctg tagaagttga tgatagagat aactatacaa ctggatggaa
    12481 atttaatgag tgggaaatga aaggtgttcc agtaagagtt gaaattggac caaaagatat
    12541 agaaaataat caagctatgg tatttagaag agatacttta gaaaaggatt ctatgccttt
    12601 agaaggatta gctgatgcta tatgtgattt atttgatgtt atacacaatg atatgtttga
    12661 aaaagcaaga aaacatagag aagacaatac atctatagtt gaaaatatgg atgagtttag
    12721 aaaagcttta gaagaaaagc caggattcat aaagactatg tggtgtggag atgcagaatg
    12781 tgaagctaag attaaggaag aaacaggagc aactataaga tgcttacctt ttgaacaaga
    12841 aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca gatagtatgg tagtaatggc
    12901 aaaagcttac taaaatacat ttaaataaaa actggggtag aaagtattta atactttatc
    12961 ccagtttttt gttttttatg agtaataaaa tgtatttgta actatgtcat aagataagtc
    13021 atagatactt atattaaaaa gaatataata gagaactatt tttaataatt acatattaat
    13081 gaaagcaaaa tattttgtta tatagatttt caataaagca aacaataaat ataaatgaat
    13141 attaataagg agtgctttta atttttagaa aataaatgac ttaaaagaaa gatttatgaa
    13201 attaaaacta ggattttctg atataataaa catgtgtatt tttaaataaa aaggatatta
    13261 ttattagatt tgattaaaaa taaacttgca aaacaatgta ctagtttaat attatagtta
    13321 tattgaataa aatatcagga ggaagtaaaa atgaatgtaa gagtaaggtt tgcaccaagt
    13381 ccaacaggat ttgtacatat aggtagtttg agaactgctt tatataacta tttatttgca
    13441 aaaaaaatgg gcggagaata tatattaaga gtggaagata cagaccaaag tagattagta
    13501 gaaggtgcta tagagaatat gcttaatgct atgaaatggg ctggggtcaa ccatagtgaa
    13561 ggtgttatat tagatgatag tggaaaagta gtacaaaaag gagaatatgg tccatatatt
    13621 caatctcaaa gattagatat ataccaagaa tatataaagc aacttttaga tagtggaaaa
    13681 gcttattact gtttctgtac aaaagaaaga ctggatgaag taagagatgc tcaaagagca
    13741 gcaggagaaa cagctaaata tgatggtcat tgtaagaatc tttctaaaga agaagtagaa
    13801 gcgaatataa aagcaggaat accatatgtt ataagattaa gattgccaga gaatcataca
    13861 attaagttta cagacttagt tagaggagat atggaattta atactaatga cttagatgac
    13921 caagtattaa tgaaaacaga tggatttcca acttatcatt ttgcagtagt tgttgatgac
    13981 tatttgatga aaattacaca tgttataaga ggagaagaat gggtatcatc tactccaaaa
    14041 catgtttatt tatatgaagc ttttggatgg gaagcacctg tatttgtgca tttaccaaat
    14101 atacttaata aagaaaagaa aaaattaagt aaaagacaag gtgatgtagc tgttgaagac
    14161 tttaagaaaa aaggatattt accagaggga ttagtaaact atgtagcatt agttggttgg
    14221 tctcctgaag ataatcaaga attgtttaca atggaggaat tagaaaaagc cttctctgta
    14281 gaaagagtct ctaaaagtgg tggagtattt gatactgaaa aattaaattg ggttaatcag
    14341 cattatataa aagatggtga tgatgcttat ctaacagact tagcaatacc atttttaata
    14401 gaagatggat ttataacaga agaagaagct actaataaat atgatttctt aaagagtatg
    14461 atatcagtac ttaaagagaa attacaatat gtaaaagaag ttactgaaca tgcaagtata
    14521 ttttttggag ataaaataga ggtagaaact gaagagggaa gagagttctt aagattagaa
    14581 catataccta ctttaataga tgctttgaga gaaaaaatag aaaaaacaga ggtattaaat
    14641 gctgagtttg ttcaagctat gcttaaagaa atacaaaaag agtacaaaat aaaagggaaa
    14701 aatttattta tgggctcaag aataattttg acagggcaaa tgcatggtcc agatttacca
    14761 aaagtaatgg aagttttagg aaaagaaact tgcttaaata gaatagctta tgtaaaaaat
    14821 aatatattat agtcataaaa gtccgctaat ttagaagcgg actttttttg tgttagtatt
    14881 tggtgaaaat atctgcttaa gaaaatgaag gtatttaagg taataaattt aatatataaa
    14941 aagcaaagca aaaatataaa taaaatatga ataataaact aataaaatat taagcataga
    15001 agaagggaat ctattatttt agtttgattt aattattaag ggggtaatta ctatgtatag
    15061 taatattgat gatgttaaaa aggaattaaa ggagctatgc ttagaatatg ttacaatttt
    15121 agaaaaatta aaagatgaaa aaatgattac agaagaaact tttgaaaagt gctcttctca
    15181 aaaaaaaata tttttagaag aacaatagag gatggtaata gtattattat tatgtagttt
    15241 gaaagatagt acgaaaaatg atagtaatac tattatttat tgcaattttc aactataata
    15301 taatatataa tgttaagaag ataatttata aaaatacagt gaggtgatga gtgtgaaagt
    15361 ttataataca ttgactagaa caaaagaaga atttgttcca ttagaagaag gaaaggtaaa
    15421 aatgtatgtg tgtggtccaa cagtttataa ctatatacac ataggtaatg caagaccatt
    15481 tataatattt gatactctaa gaaggtattt agagtataga ggatatgatg taacttatgt
    15541 acaaaacttt acagatgttg atgataagat tataaataga agtcatgagg agggaatatc
    15601 tcctgaagaa gttgcggcta agtatataaa agaatatttt gtagactgtg atgggctagg
    15661 aataaaaaga gctactgttc atcctcaggt tacagataat atacaacaga ttatagagtt
    15721 tataaaagag ttggaagata agggatatgc atatgcagta aatggtgatg tatattttga
    15781 tacaaataag tttgaaggtt atggaaaact ttcaggtcaa aaacaagaag atttagaggc
    15841 aggggctaga attgaggtta atgaccaaaa aagacatcct atggactttg ttctttggaa
    15901 ggcaaaaaaa gaaggtgagc caggatggga tagcccttgg ggagaaggta gaccaggatg
    15961 gcatatagaa tgttctgtta tgtctaagag atatttaggt gaaacaattg atatacatgc
    16021 aggtgggcaa gatttaacat tcccacatca tgaaaatgaa atagctcaaa gtgaagctag
    16081 aagtggaaaa acattctcaa aatactggat gcataatgga tatataaata taaatgatga
    16141 aaagatgagt aaatcaaaag gtaatttctt tacagtaaga gatatatcaa aattatatga
    16201 tttagagata gtaagatttt tcatgttatc agcacattat agaaatccag taaactttag
    16261 tgatgagatg ttaaatcaag ctaaagcagg tcttgaaaga ctatataata ctaaagaaaa
    16321 attagaattt acgctaagta atttagtaga atctccttta acagaaaaag aagtagagct
    16381 tgttaaagaa ttagatgact ttagacaaaa attcatagat gctatggatg atgatgttaa
    16441 tacagcagac gcagtaagtg taatatttga attagctaag ttaataaatt ctaatgtaga
    16501 tgaaaattct tctttggagt ttgctaaaaa atgcttagat gaatttaatg aacttacagg
    16561 agtattaaat attgtaaata agaaaaagga tactgtatta gataaagaca ttgaagaatt
    16621 gatacaaaaa agaactgatg caaagaaaaa taaagagttt caattagctg atgatattag
    16681 acaacaatta ctaggtatgg gaattgtatt agaagacaca agacaaggtg ttaagtggaa
    16741 aaggatataa aaatggaaaa aactgaatta gttactatgt caccattagt tttagcatac
    16801 ttaggtgata cagtgtatga gacatatata agagaatatt taattagaca gaatacacaa
    16861 agaaaagtta atgatttgca taaactagct ataaagtatg taaaagctaa agctcaggca
    16921 actataattc atgaaattga aattgagtta actgaagaag agagtaaaat ttataaaagg
    16981 ggaagaaatc aaaagtcaaa tacttctcct aagaatgcag atattataga ctacaaacat
    17041 gcaactgggt ttgaggcatt agtgggatat ttgtatttaa ataatgaaat agagagacta
    17101 caatatatta tcaataaagg aatcaaaatt attgaaagag atatgtaaat attaaagagt
    17161 gttgtcttaa taataagtaa taaggcaaca ctttttttat aactaatgta taataaatat
    17221 atagaaatta gtaatgagaa aggataaaaa gttatgaaag taaaattaat atcacataca
    17281 cctgaaccag aaaaagtaat agctatggca gctaagctat gttattcacc agttgggaca
    17341 gacgaaatag aaaaagactt aacagatgaa agtatagaga aatttttaaa tatgttatta
    17401 agtataggtc atgggtcaat attagaacat gcttcattta cattttcaat agaaggtatt
    17461 tcaagggctt gttcacatca aattgttaga catcgtatag ctagtttttc acaacaaagt
    17521 caaagatatg ttaaattaga acaatttgag tacataattc ctccagaaat agaaaaaata
    17581 gaaaaagcaa aagagttatt tatagactcc atgaaaaaag accaagagaa ttatgataag
    17641 ttagttgaaa tattgtttga aaatcattat aatgacctaa taaaaaacgg taaaaatgag
    17701 aaaacagcta aaagacaagc tgaaaagaaa gcaatagaag atgctagata cgtatttcca
    17761 aatgcatgtg aaacaaaaat ggtatttact ataaatgcta gaagtttgtt taatttcttt
    17821 gaacatagat gctgtgaaag agcacagtgg gaaataagaa atttggctgt ggagatgtta
    17881 aaagaggtta aaaaggttgc tcctatatta tttaaaaaaa ctggtccaag ttgtgtaaat
    17941 ggaagttgtc cagaaggaac tatgacttgt ggagatatag ttcaagttag agaaaaattt
    18001 aaggccttgt aggaggtgaa taatttggcg agtatagaag gaagaaatcc ggtaatagaa
    18061 gcaataaaaa gcgacagaga aatagataaa atattaattg caaattcagc taaagaaggt
    18121 tcaattaaga aaataatagg aatggctaaa gataagaata taattattca gtatgttgat
    18181 aaacataagc tagatgaagt aagtacaagt cattcacatc aaggtgtaat agcttatgca
    18241 agtgagtata agtattatga attagatgag ctaatagatt tagcaaagaa taaagatgaa
    18301 gacccatttt tcattatatt agatgagata acagacccac ataatttggg ttcaataata
    18361 aggacagcag atgcagtagg agcacatgga gttatcattc caaaaagaag gtctgtacac
    18421 ataactcctg tagttgccaa agcatctgct ggagcagtag aatatatgcc agtttgcaag
    18481 gtaaccaata tagttaatac aataaaaaga ctcaaagaag aagggctgtg gattgctgca
    18541 gctgatatgg atggagaaac tttttataaa caaaatctta ctggtcctct tggtgtagta
    18601 attggaagtg aaggttttgg aatatctaga ttggttaaac aaaactgtga ctttatagta
    18661 aaaatgccaa tgataggtaa tgttacatct ttaaatgcat ctgtagcagg tggaatactt
    18721 ttatatgaga tattcagaca aagactggat aaaagtaaat aggcatgaaa aaaaatataa
    18781 atcattactt aataatagat ggctacaata ttataaatgc gtgggacaat ttgaaagaac
    18841 tggctaaaga ggacttagaa gattctcgag aaaagcttat tgatgacatt atagaatttt
    18901 ctgagtttat gggttataag actataatag tttttgatgc ttataatgtt aaaaattctc
    18961 gagaaaaagt tgaaaaaaga aaacacataa caatagttta tacaagagaa caccaaacag
    19021 cagatagtta tatagagaaa tttataactt ctttatcaaa gtatgatgat gtaaaagtag
    19081 ccactaatga ctatgcagaa cagcaaatga ttttaggaaa aggtgctact agaatgtctg
    19141 caagagaatt aaagttagag ttagatcgtt ccaagaataa aatgaaggaa aaaaatatcg
    19201 gtttgaggaa aaaaattcag cgaaattggt tggaggaaag gttagataaa gaaacattgt
    19261 cgaaacttga gaacattcgt agaaagcgtt gaaattacta ggttctttag actataatat
    19321 acctataaat ttgttttata tatgggggag atttacatgt tggtagcaaa agaaaaaagt
    19381 tatgagttag tggacaattg tcagcaggat gagtataaca ttgtattaag agcaagtgaa
    19441 ggggataaaa tagcactaga gtacattatt actaaataca gaaattttgt aaaggcaaaa
    19501 gcaaagtcat attttttaat tggagcagat aaagaagata taatacaaga aggaatgata
    19561 ggcctttata aggcagttag agattttgat ggaagtaaga ccaattcatt taaatgtttt
    19621 gcggagatat gtataacaag acaaataata actgctataa aaactgcaac aagacaaaaa
    19681 catatacctt taaattcata tgtttctttg aataaaccaa tatatgatga agagtcagac
    19741 agaacacttt tagatattat agctacaagt atagtaacag atccagaaga gcttataatt
    19801 agtaaagaag agttaaaaaa tatagaatca aaaatgaacg aattgttaag tgacttggaa
    19861 ttagaagtac tagaattata tttaaatgga aagtcatacc agtttatagc ggacaagctt
    19921 aaaagagatg taaagtcaat agataacgct ttacaaaggg ttaagagaaa attagaaaaa
    19981 catcttgaaa acagaaatga ttaatagtaa aatacttacc ataagcttat tgtgagcaaa
    20041 attaatttat atcgaaagat aagggaggaa attcaaaatg gctaaagcta aatacgaaag
    20101 aacaaaacct catgttaata tagggacaat aggacacgta gaccacggta aaactacatt
    20161 aacagcagca ataacaaaaa cattatatga cagatatcaa ttaggagaag cagtagattt
    20221 cgcaaacata gataaagctc cagaagaaag agaaagagga atcacaatat caacagcaca
    20281 cgttgagtat gaaacaccaa atagacacta tgcacacgtt gactgcccag gacatgctga
    20341 ctacgttaag aacatgataa caggagcagc acaaatggac ggagcaatat tagtttgttc
    20401 agcaacagat ggaccaatgc cacaaacaag agagcatata ctattatcaa gacaagttgg
    20461 agtaccatat atagtagtat tcttaaacaa atgtgacatg gtagatgatg aagagttatt
    20521 agagttagta gagatggaag taagagattt attaacagaa tatgatttcc caggagatga
    20581 cactccaata gtaagaggtt cagcattaat ggcattagaa gatccaaaga gtgagtgggg
    20641 agataagata gtagaattat tcgagcaaat agatgagtat ataccagctc cagagagaga
    20701 tacagataaa cc
//
LOCUS       DAMJYC010000067        20445 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJYC010000067 DAMJYC010000000
VERSION     DAMJYC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.068"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(501..2105)
                     /locus_tag="PC472_002665"
     CDS             complement(501..2105)
                     /locus_tag="PC472_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2727982.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDKNPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2491..3417
                     /locus_tag="PC472_002666"
     CDS             2491..3417
                     /locus_tag="PC472_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2727983.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3537..4535)
                     /locus_tag="PC472_002667"
     CDS             complement(3537..4535)
                     /locus_tag="PC472_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2727984.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4639..5076
                     /locus_tag="PC472_002668"
     CDS             4639..5076
                     /locus_tag="PC472_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2727985.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5162..5749
                     /locus_tag="PC472_002669"
     CDS             5162..5749
                     /locus_tag="PC472_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2727986.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6049..6669)
                     /locus_tag="PC472_002670"
     CDS             complement(6049..6669)
                     /locus_tag="PC472_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2727987.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6677..7444)
                     /locus_tag="PC472_002671"
     CDS             complement(6677..7444)
                     /locus_tag="PC472_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2727988.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7485..8306)
                     /locus_tag="PC472_002672"
     CDS             complement(7485..8306)
                     /locus_tag="PC472_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2727989.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8665..10575)
                     /gene="phnW"
                     /locus_tag="PC472_002673"
     CDS             complement(8665..10575)
                     /gene="phnW"
                     /locus_tag="PC472_002673"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2727990.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYVQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10854..11639)
                     /locus_tag="PC472_002674"
     CDS             complement(10854..11639)
                     /locus_tag="PC472_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2727991.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11856..12086)
                     /gene="dltC"
                     /locus_tag="PC472_002675"
     CDS             complement(11856..12086)
                     /gene="dltC"
                     /locus_tag="PC472_002675"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2727992.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12105..13253)
                     /gene="dltB"
                     /locus_tag="PC472_002676"
     CDS             complement(12105..13253)
                     /gene="dltB"
                     /locus_tag="PC472_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2727993.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13253..14767)
                     /locus_tag="PC472_002677"
     CDS             complement(13253..14767)
                     /locus_tag="PC472_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2727994.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14888..16078)
                     /gene="dltD"
                     /locus_tag="PC472_002678"
     CDS             complement(14888..16078)
                     /gene="dltD"
                     /locus_tag="PC472_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2727995.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16366..17385)
                     /locus_tag="PC472_002679"
     CDS             complement(16366..17385)
                     /locus_tag="PC472_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2727996.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17561..18100)
                     /locus_tag="PC472_002680"
     CDS             complement(17561..18100)
                     /locus_tag="PC472_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2727997.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18201..18881)
                     /locus_tag="PC472_002681"
     CDS             complement(18201..18881)
                     /locus_tag="PC472_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2727998.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTEIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILETLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18910..20199)
                     /locus_tag="PC472_002682"
     CDS             complement(18910..20199)
                     /locus_tag="PC472_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2727999.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6740 a         3520 c         2099 g         8086 t
ORIGIN      
        1 aacatagtcc tatatatcta ttatttggat agaattattt tttaaattag aattattttg
       61 atgaatgatt caagtaatag gtatgaaatt gaatctttta gaactgctta caaaaaatac
      121 tcaaagaaaa atagtaattt tctgagattc acaatatatt ttatatgaaa tgttataagg
      181 taatctattt aataaattta aatttgctat tatagattta gttccaaagt cttactgcaa
      241 agtaatatac atagtttttt aataaactaa taagttcaat taataactac ttaattgttt
      301 aatatagtac atagataact ttaataaatc gacagttatt taaaaaggta gaatcaataa
      361 aattatactt ataaatcttc aatacaataa ttaaaattta ataaaaatat aattgttgat
      421 ttaactgtaa tcttatttaa ttctacctaa cttattatat aaaactattt tttatagatt
      481 ataaaatatc taactttcat ctacttcaaa gaatacaata cttccccttc tcttatagtc
      541 tgcaatactt taatatcttt tattttcata gggtcgattg taagtgggtt tttatctaat
      601 attatcaaat tagctaattt cccttcttta attgaacctt ttttatcttc ttcaaaatat
      661 tgatatgctg catttatagt aacacccttt aaagcatcta atgggcttat cttttcattt
      721 tctcctattt gtataccttt ttttgtaatt ctattgacag cacaccatac tgtttccagc
      781 atattaggtg ctattactgg tgtatcctga tgaagagtgt aaattaatcc cttttctata
      841 gttgttttaa gtgggctaat cttaaacgct ctatcttctc caaggttttt gatatgaata
      901 tcaccccaat aatatgtgtg agctacaaag tatgaaggta tcatatttat tactttcata
      961 tcatcaatct ggtctgctct tactgtttga gcatgtatca taactggtct tatattattt
     1021 tcactacttt gctcttttaa gtttaaaact ttctcaaacg catctatcaa ctggtctgct
     1081 gctgcatctc cattacaatg agttaagagt tgcatctttt cttttagtga aatatctata
     1141 aacttttcta cttcttcatc tttgtatata ggatatccac aataaccatc atctgaattt
     1201 tcatatggtc tactcaacca tgctgtctta ccttgtggag aaccatctaa gaaaatctta
     1261 taacctccta ttttaaatct atttatatat ttttttataa actccctatt attatctgct
     1321 acagacttac tttttttcaa atttacatat cctacaacat ccatttttaa tttattttga
     1381 tttgccattg cttttaaaat gttaaattga ttttcttcca ttaaaccttc ttgagctgtt
     1441 gtaataccat agcttagata tatgttctgt gctttttcaa ttgaattaaa aatctcattt
     1501 gatgatggtt gttttatttt agatgctaca ttaaaaaatg cattttcttc taaatatcca
     1561 tttggttctt cactgccttc tactctacct atatgtccac cttctgggtc acgtgtttca
     1621 ttggtaacac caagctgttt taaccccaat gtatttgcaa ctcccatatg accagaagca
     1681 tgtgaaatta gtattggatt ttctgaagat gcactatcga gtacactctt atctggatgc
     1741 ttattttctt ctaaaaaatt attatcatat gaaaatccaa ttatccagtc tccttttttt
     1801 atattattcg actctttgaa atcctgtatt ttttttacta tgtctttaaa actggttgca
     1861 tcttctaaag gaactagctt tagagtagta gcaaaagcta tcaagtgact atgtgaatct
     1921 ataaaagatg gcatcaatgt cttaccttgt aaatctataa tttctgtatc tttatttttc
     1981 aaagcaaata cttcttcttt tgttcctatc ttttttatta ttttgtcttt tataagaata
     2041 gcatctccac atattgaatc ttccattgtg atgatatttc cattgtaata aatttttgct
     2101 ttcataaaat tctccttacg ttgtctactt taataaattt ttttcattat tttctgctta
     2161 attatattat cttctttata aactttttct ttagagttta atcatataaa agttttattt
     2221 ttaattattg tttgccaaga agtaaatact attcattgta aataagacaa attaatagga
     2281 tttatactaa ttgaaaaaat attataagtt gttaaattta aatatctaaa ctcaattata
     2341 tctgtacaat tgtaattttt taaatcatga tgtattattt taaatataca gttacattat
     2401 tattcacaat ataagtatct tagttctcaa ataacatctc atgtaatcta ttcaatgcaa
     2461 aaaatttaaa tattttttag gaggtttttt atgagcaagg aaaataaaaa tgtaagaatt
     2521 gctgttgtcc aggcgtcacc agtcataatg gatttagaaa aaactgttga aaaagcatta
     2581 ggattaatta aagaagctgg tagaaaaggg gcaaatatcg tagttttccc tgaagcattc
     2641 atacctgcat atccaagagg tctttcattt ggatttgttg ttggaagtag aacaatggaa
     2701 ggtagagaag attggaaaag atactatgat aattctgtac cagttcctag tgctacaaca
     2761 gatttattag gaaaagcagc acaagaagca ggagtatatc tatcaatggg tataacagaa
     2821 agagatggta atgatataaa ttgtaccctt tattgcacaa acttattttt ctctccagaa
     2881 ggtaagctaa ttggtaaaca cagaaaactt aaacctacag gaacagaaag atgtatttgg
     2941 ggagaaggag atggaagtac tcttacagta gttgatactc catatggaaa aatgggttcg
     3001 ttgatttgtt gggaaaatta tatgccatta gctagaacag ctctttatgc taagggtgta
     3061 aaactttaca tagcccctac tgcagattca agagaagagt ggcaagcaac tatgaaacat
     3121 attgctttag aaggaagatg ttttgtaatt ggatgcaatc aatatgttga aaaaaatatg
     3181 tatccaactg accttaacta ctataaagag ctagatgcag aaccagaaat tatgtgtcca
     3241 ggaggaagtt gtatagttga cccttttgga aaatatgtag caggtcctat tttcaataaa
     3301 gaagaaatgc taatagctga tttagaccta gaaaaaatag tacttagtag actagatttt
     3361 gattcagaag gtcactattc aagaccagat gtatttgaat taattgttca tgaataatct
     3421 ttttacacaa acatctacat aaatcttttt acataaaaaa atgagatact cataagtaaa
     3481 tttcaagtat ctcatttttg cttaaaacct ataatcctaa ttataaattt attcacctat
     3541 attttttcta aatcaactac tgttcttcca acatgttttc ctgccattat tgaatctata
     3601 ctatcattta actcttccaa tgatactaca tttaccatat catctaaatc atctaatttc
     3661 cattcatttg ataataaatc ccatattttt aatcttaagt tcattgggca atccacagaa
     3721 tctataccat ataaagtaat tccctttaat ataaatggat atactgaact tgtaaaatca
     3781 actcctcctg cattaccaca agttgttact gctccacaat aattagttgt ctttattgct
     3841 gttgctagag tatttcctcc tactgtatct attactccag cccatcttgg tctaagcaga
     3901 ggtcttcctg agttatcatc cacttctgac ctatgtataa catctttaac acctaagttt
     3961 agtaatactt ctttttgttc ttctaatttt cctgttgaaa ctacaactga ataacctaat
     4021 ttaatcaata ttttagttgc aattacagca actcctccag taccaccagt gactaataca
     4081 tctcccatat tcttgtctac agaatctata aatttataca cagataaagc tgctgtaaac
     4141 cctgctgtac cataatccat tgcttctttt agtgaaagat ttttaggaag tttaactacc
     4201 caactagaag gaactcttat gtattctgaa aaaccaccag aagtattcat tcccaagtca
     4261 taacctgtta ctaaaacttc ttctccaatt ttaaaatcat cacaactact agattctact
     4321 ataccagatg catcaattcc tggtgtatga ggatactttc tcgtaacacc cttattacca
     4381 tttgcagaaa gtgcatcctt gtaatttaaa gaagaatatt ttacttttat taaaacttcg
     4441 ccttctggca atgtatccac ttctctttca actatatttc ttctaaaatt tccttcttct
     4501 tctgtaatat atagtgattt aaatttttta ttcataaatt tcctcccaat acatttagtt
     4561 ttatgttaaa ctaattatag tttataaata gttttatgtc aaactaattt aaatagatta
     4621 aagattggag aataatttat ggaagataaa tatattgtac atttcataag taaaacaaag
     4681 gctagtatga ttaaattcat tgaaaataaa ctcagtaaaa atggtttagg tgagttaata
     4741 cctactcatg gaaatatttt aactgcactt tatgaaaata atggaatact tacaatgaaa
     4801 gaaattgcag ctaaaatagg aaaagataaa tccactgtta cagtgttggt taataaactt
     4861 ataaacctag gatatttaga aagacaaaaa tgtacaaatg acaaacgtat cacgtatata
     4921 aagcttacaa aaaaggcact gcttatagaa gatactttta atagcatctc aacacaagtt
     4981 aaagagacgg cataccataa tattacagaa aatgaaaaac aagaatttct tagaatttta
     5041 aaaaaaatta atgataattt taaaaatgct gactaaattg agcataatta tatctcctat
     5101 tggaataata aataaaaaga atcaaatatt gtaaaaattt attatgatag agaggtagaa
     5161 tatgaattta aaagatagta aaactaaaga aaatttatta agagcttttg caggtgagag
     5221 tcaagctaga aatagataca atatttctgc atctgtagca aaaaaagaag gattatatat
     5281 aatagagtct ttatttaatt acactgctga ccaagaaaaa tcacatgcaa aacaattctt
     5341 ttctaaatta aaagaatttg ctggtgaaaa tataagtata tcaggagcat accctgtaga
     5401 tttatatgac aagacagcac aacaccttaa agctgcacaa cataatgagc ttcaagagtg
     5461 ggatgatgta tataaaaact ttggaaaaac agcacgtgaa gaaggatttg aatctatagc
     5521 taagttattt gaaaatatag ctgaaataga aaaagttcat ggtgatagat ttggtagata
     5581 tgctactgat ttagaagaag gtactttatt cagaagaaat gaagatatac aatggatatg
     5641 tacacattgt ggccatatac atataggtaa agaagctcca aaagcttgtc ctgtatgtct
     5701 tcatccacaa ggatatttct tagattttaa aaactcatta tttgaataaa ttaaggcaat
     5761 aattctattt aaacaattta ctatatttat atcaagtatt tttataaata ctgtattatc
     5821 ttaataacaa taaaatgaac cattgatatt actataagta gaaaatgtag tttaaataca
     5881 atttatagta attcatcatg gttcatttta ttatttacta atccctataa cctacttaaa
     5941 tctatcttta acttttacac actcacataa aatctcatct ttaaactaaa tatttaaata
     6001 aagtgtattt taagtatatt attataatac attatataag ataatacact atgataactt
     6061 tatacttata tcatttattt cttttgtcaa gtcaacctca ataatcttaa ctacatcttt
     6121 ttcaaacttt acttttttat aatccaaaca tcctactacg aatggaactc caaactttct
     6181 aaacaaataa atctttccac tttcattgta tattgatgta aaattttctt ctattatatt
     6241 cactatcaat tttaactctt caaaagttat tatagattta actaaaaact tctctattat
     6301 gctcaatata agcagttctt ttttacctat gctttgctct tttttatata aattttgaaa
     6361 tatttctttg gtataattac atataacttc tttactgaga ataaaatcaa tgtcaaattc
     6421 tttattaata tcctttttca taaacatatt tgcaatatca tcaagagaag tctcttcttt
     6481 catagacaat attaactcta ttctttctag tattttatct cttggaaaga aagtctcttg
     6541 acctgtaaaa gctgattttt ttataaacca ttcctctggt attatgtttt ttcttttcca
     6601 tctatataat tgaccataag atatattagt tttactcaat aaatctttct tagatattaa
     6661 ttcttccata acttttctag cccaccttta tactgttttt tacagtagaa ttatctgaaa
     6721 ctgataaatt atcactgtat tcaatatttc caacgtcaca attatctact agagatatgc
     6781 ttttacctcg tatatctttt attgatgcat cttctaattc aatagtatct ccctctatca
     6841 aattacattt aaactttcct gaaaaaatat taatgatttt aattaatgaa aaagcatctt
     6901 tgcttcttcc tatttttata ttagttgccc ctatctcttt acaataagaa cctcctcctg
     6961 aataaacata tatattttct ccatttaaaa gaccatcaca ttttattact ccatatacct
     7021 ttatatcttc acattctata tttgatttat ttctcatttc gccatagatt tttatatctt
     7081 gtgcatacat attttcattt acacttaatt gtcctcttat tgtaacatta ttacatttaa
     7141 gtttgccatc cactttctgt tgagcagtta taactaagtc atctatttct gcatctccct
     7201 ctatattaag ttgaccattc actcttacac gctctgcttt aagattgcct cttataatag
     7261 tttttccgtt aacaacaaaa ttttttatat tgatgtcacc ttcaaatttt gcatctccaa
     7321 ggacttttac ttcgttactt ttaacatttc cttttgattt agaacttccc acgattttta
     7381 tattttcata ctcaccacta cttataaagc cttctccaag tatttttata gacccttttt
     7441 ccatattatt acctcatttt aaattttatt tttaaaccta tttattactt atccttcttt
     7501 aattttattt tttcacttat attctttatt tcaccaacta ttgaatcttc atcaatctct
     7561 atactttcac tgtactccaa actttctaca taacatccct tgcctatttt aatatttttt
     7621 cctcttacaa ctttgacttc actattttct aagcttattt catctgcttc tataatattt
     7681 gcaaacaatt tactttctgt atactttcta attgataata gacctaaact tattaaattt
     7741 gaagacactt tttcaaagtt gatatgcttt ttttctagca tacgaaattt tttgcaataa
     7801 tctccatgcc ccttttttat atttatacta cttgctccaa cttcattaaa attagaagtt
     7861 ccatttaaac taatattaac ttcttcacaa ttcaaaaatc cattacactc caagattcca
     7921 cttaaattga tattttcaca atctatattc tcatttgctt ttaaatatcc ttctccatga
     7981 ataatatccg attttatact tccttctaca ttaataattc cactagtatc aatttcaaca
     8041 gaattaatat ttcctgtaca tctaagaagt cccgatatct caacttttga tgccagtatg
     8101 tttccttcta ctttacttaa accagatgta aaaaattcat tacattctgt tgtcccaaaa
     8161 acttttgctg aaccagaaga atcaaattta ctacatttta cacttccttc tattttgcct
     8221 gttcctgata tattaacatt atcaaaaatt ccacctttac attttccaac accatcaaat
     8281 cttagtgaac ttaattcttc attcataatg atatcctttc tttaccgtat tttatcttta
     8341 aacctaatat aacaaaacat tgttacgttg tcaagtatga taataatact tagtaaagca
     8401 actatcattt attttctata aataggcata aaaataatag ggaaattctt tatttaaact
     8461 ataaatctaa tttcttagat tgtggtttaa ataaaatata tccctattac tcaatcttta
     8521 tatattcatt gtataaactt ctaaaatata agtctttaaa ttctactgtc aatacttttt
     8581 atacactgtt tgtgctatca tctcacacac ctaaaggagt gggctttccg cactattctg
     8641 taacctacaa gacactatct tcttctatct attgataaat ttttctatta catcagctag
     8701 tctctccata tcagttggat aaacttcacc tatgctacct attctaaatg tatctatatc
     8761 agtcaattta cctggataga taacaaatcc attatctttt aaataagtat aaaattccat
     8821 aaattcaaac tttgcatttt taggatataa gaaagttgtt attacaggtg attgagcatt
     8881 atcattcacc aaagtatcaa atcctaaaga ttttaatctt gaagctattg taaactgatt
     8941 ttctttatat ctagcatatc ttttttcaac agaaccttct tcttccaatt ctaataaagc
     9001 ttgataaaaa gctcttacaa catgtgtagg tgaggtaaat ctccatttac cattattttt
     9061 ctccatagtc tcccattgca catacacatc aagagataat gatttagcta tacctttaca
     9121 tttagataat ttctctttat tagctattat aaaaccaaat ccaggtactc cttgtataca
     9181 tttgttagaa ctactaacta aaaaatctat attaaaatct tcaacatcaa tctcaattcc
     9241 cccaaaacta ctcatagcat caactatata aattttgtta tattcttttg caagtttccc
     9301 tacctcttgt ataggattta atctacctgt agtagtttca cagtgaacca ttgatatatg
     9361 tgttatatcc ttgttttctt ttaataaatt ttctactaca tttaaatcca ctgcttctat
     9421 atctttaaat gtgcaatcaa caaattctat gttaagataa ttacatatat ctttcattct
     9481 ctttccatat gctccatttg caattacaag aagtttacca tcttttgaaa tagtacttcc
     9541 tattatagct tctactgaaa aagtcccact tccttgcatt aatacacttg tgtatttatc
     9601 tgtattttgg gttgctagag atactaatct tcttctaaca tcttgtacta ggttattata
     9661 ttcaacatcc caagtacacc aatcttttaa catagatgct cttactgatt tagtagtaga
     9721 tagtggtcct ggtgtaagta atatgtaatc attttctggt acaaaatctg atttaatagt
     9781 ttcattctct atttttatta gtatatcttc taactcagcc atagtttcta ttacaaaatg
     9841 tgctcctgcc tctttaaatt tctttgaaac tatactcatc ttagctttta attcttcctt
     9901 atccatgttc tctacttctt cttgtgtaag ccctaactca cttgaacctt ttattacagc
     9961 aacactccac attcctgcat ttacgccttc ttttacatca cttattgtat ctcctacttt
    10021 taccatagaa ctcataggag acacccctaa agcttcagca tttttatagc acatccaagg
    10081 atatggtctt ccttgagata cttctgatgg tgttacaaga aaatcgggtg agtatccttt
    10141 ctttgctgca tttggttcaa ctatgttcat catctctctt gtataacccg ttgtagaacc
    10201 tatttttaat ccattttttc tcaacttttc aatagtttca acaacatgag gaattggagt
    10261 cgtatactct tctaatgtct caaataacat tggctcaaat tcagcataaa gttcatttac
    10321 atcatcttca gttggcactt taccaaactt atctgaccaa atattcttta ttctatccat
    10381 ttcacacatt tctcttatat ggtctatttt taattttccc ataggttttc ttgcttcttc
    10441 cattgttaca tctatacctc ttcttttaaa tatctctata aatacattaa gaggtgcaaa
    10501 acatccataa tctactgttg ttccagccca atcgaatact actgctttta ttttttctcc
    10561 atatatcttt ttcatattta tagcctccat agattctttt tagaattttt gtgtttttaa
    10621 ttgtttttac ttttaaagta taactttcat atgttaatta tacatcatat ttaggttaaa
    10681 tatatattaa atttctgttt ttagttgtat ttagttgttt ttattttgta tcatattccc
    10741 ataaaatcct aagttacaat ctaaaattta ctttctaatc ttaatctatg aactgactat
    10801 attttatgaa tttagttgta ttgagcatag ttgtattata taaaactaag ggtttatttt
    10861 gcttctatcc atgttatatt tatctcattt aacttatcct tccattcatt cggaggttct
    10921 acatcacaaa taacaaaatc taattcctct aaatctgcta tcttatacat actagcaatt
    10981 cctattttag aactatcaca taatacaaaa ctttcctttg aattttttat atatgctttt
    11041 gacaacaatg ccttattttg gtctatacta ctaattccaa aactatcaag tataccttca
    11101 ctagatataa aagctttatc tacgtataag tttttcatag catctattga tattggacat
    11161 gctgtccttt ggtgttttaa atttactttt ccaccaataa atataacttc tccatcaaac
    11221 aaaccttgat tctcgtaaga aatcaatttt gacagaactg gataagagtt tgtaataatt
    11281 tttaaattgt ttacagatat tatataatct acaatctgca aattggtagt accttcatct
    11341 atgataattg actcattgtc atttacaaac ttagctgcat atttagcaat actttgcttt
    11401 ttatctaggt tagtattgtt tctctttata tgttctggtt caatcactcc ataatttatt
    11461 tttatagcac caccatatac cttttttaat ttattttcat tttctaattc ttccaaatat
    11521 cttcttattg tttctgatga tacttctaat ctttttacta attctttagt gtttaccttc
    11581 ccttttgaat ctagtaattt aagtatttct ttttttctat ctatcgaatt taaagacatt
    11641 cattttcctc ctaaatataa tatatttttc tcctataata ttacaatgat acctaagatt
    11701 tatgaaatca tatttatgta ttaaattttt tacttaatta aagttatttt caaaagactt
    11761 tgtattatca ttaaaaaagt ccatcaaata ttagtatcag atggactttg taaaatttta
    11821 ttttataaat taatatttaa agtctaacta ctaatttatt ttcttgtctc caagaatttt
    11881 actaaattat ttacagtaga catatctttt ctctctaaat cagttggttg aagctctatc
    11941 cctaatctat tttcaatttc aagtaaaact tcaattatag ctaatgagtc taaaagttcc
    12001 gtctcaaata aattaaggtc taaatcttct cttatctcat cagtccctaa tacatcttca
    12061 aatatttcta taactgtttc ttgcataaat tttcctcctt aaaattaagc taaataacct
    12121 gagaatatca gcaatccaaa acacacaata tggaatgtaa tgaaaatctg cactctctcg
    12181 aaccatttat cttttttatg ttttttataa aatttagatt ttcgctgata tatatcagtt
    12241 aacacaagtg ccaatccttg gtatacacca taagcaacat aataccaagt tagaccatgc
    12301 caaaatccca ttgttatcat ggttatcatc tgagccacat gagcagcagt agtccttttc
    12361 ttaaatcttt tctttctcat tgatgacata acaaatctag aaaaaatata atctccaaac
    12421 catcttgaaa gacttatatg ccatcttgtc cagaactctt tcatatcttt gctgataaat
    12481 ggcttgtcaa agtttatagg tacttgtatt ccaaatatgt accctgtacc tatagcaaat
    12541 aaactatatc ctgcaaaatc aaagaacagg tataaactat atgcgtacat ataacttaaa
    12601 atatttataa aactcatatc tttaggtatt cttgataccc aatatgtatt tatcaaaaat
    12661 gctataacaa acttataacc tactcccatg acaatatttt ttattcctgg cagtaagtat
    12721 tcttctatat actcttttct agagatttgt ttttccaagt cctgttcaaa tcttcttgac
    12781 ctatcaatag gtccagaact taaagttgga aaaaataaca taaaataaag catctttgaa
    12841 attttaactt cttttatagc tccatcataa atttcaataa ccatttgtat agttctgaaa
    12901 tttaagtatg atattccaat aaatccaatt attccaaaag aagtaactgg agatatttta
    12961 tttattatta ttgggagcat tgatgcaaac aagaatcccc aatatatgta tttattttta
    13021 gtttttcttc ttacatattc atatccttta actattatta cttcaagaat aataaatatt
    13081 accaagtact ttagttggac atcaattcct actattaaga atatcataaa caaacttgcc
    13141 aacataccat aatactttat attcttgccc attagaccta aaattactgc tggtattgat
    13201 gttaataata atatataaag ataaaaataa tctccatatt gtgaaaaaat cattaaattt
    13261 cctccataag tttctttctg tctattttac cattgatatt tgttggaaac tcagagatta
    13321 ttgaaatatt tcttggaatc atatatgaag gtatatactt tccaagttcc ttttttatta
    13381 tcatcccatt tttaatatta cttaaatcat tcttttcatt taactcaaca atacctttta
    13441 agtaagcaat tttttcgtcc ttatatactg gtaaaactac tgcatttttt acattatgta
    13501 cttttctcaa attattttct atgtcttcta tttcaattct aaatccattt aatttaatct
    13561 gaaaatcttt tcttccacaa taatatatat ttccatctaa aagatatccc atatctccag
    13621 ttttataagc tctccacttt acaccatcta tttcatcata gaaaaatact tcatcagtct
    13681 tttctttatt attgaagtaa cctttagaaa ctgatggtcc aattattata atttcaccct
    13741 tttcattttc tttaagctca tttccatctt catcaagtat ttttatttta caatttgaca
    13801 tagggtatcc aactggaaga cttttttcat catctatagc cttttgagtc atatcattta
    13861 cacttacacc aacagtagcc tcggttggtc catatccatt gataattctt gtgtttggaa
    13921 atctactcat aagttcttta gtaagatttt ttgaaagtgc ttctcctatg aatatcattg
    13981 actcaaggtt aggaagcatt ttactattaa actctttttc agttatacat actcctgcaa
    14041 aagaaggagt tgacacccat acagctatat ctgagatact aaattgtcta aatagttctt
    14101 tatagtctgc taatacatct tttgatattg aaaataatgt agctccatgg ataagaccag
    14161 ggtatattgt tgtaactgaa aggtcaaaag aatatgctgg ttggttcatt attacttttt
    14221 cacttccatc tatgtttaga tatgggctta tccaatctga aaagctatct aaattgtttg
    14281 aacttatttg aacccctttt ggctttcctg tacttcctga agtaaatagt atatatgcat
    14341 tttcatcatc ttttacccag ttttctttat ctaatgattt tccctgatat tcatttatta
    14401 tataatttaa tttatccata tcaattacat tgatatctcc aaaatttctt tcatcactaa
    14461 agttaaataa tacctttggt tttatctctt tagtgacttc aaaaactcta tcaattggaa
    14521 aactaatatc aagaggcaca tacgctctac ctgattttaa agctcctatc atacatgcca
    14581 ttatcatgtt ttctttattt ccataaatta ctataggagt atcttcttct ttgtatacat
    14641 cctttagaaa tactgatata gcatctgaat attcatttaa atctttataa gaaagtttat
    14701 ctccattaca cattagtgct gttctatctg tatttgaata tttttttatt ccttcaataa
    14761 ttttcatata agaatgctcc tttaaaatcc gttataaata aatggcaatt ctttaaatgg
    14821 agtaaatgtt agaactccta tcgccaatat tataaccata gttaaacaaa tactagttaa
    14881 caaatcttta ttgttcttta aatattttaa ataatctctc acagacatct acccatcctt
    14941 tcgtaccaag atgcattaca tcccttaaat aatacttatc tgacccttta tctttaaggt
    15001 ttagaacttc aaagcctttg ctttctgcta ttttttgtgc tttatcgtag tactgatttc
    15061 tttctttttc agaaatacca gtcaaattat agaatttatc catacttggg attaatacaa
    15121 caactggctt aatacctaag tcagtacata catttagcat cagtttataa ttttcaaact
    15181 ccttagatgt taataaattt acatctttgt ctcttccttt gtattgctct ataccatctt
    15241 taaaatgctc tttataatag tacttatcaa tatttagtgg atttttacca actctttttt
    15301 tagcatcttc aatggctttc tttctctcat atgaccaatt tattggtctt tttctttttg
    15361 ttgctctctt tttatctagc ttaattaatc gtttataaag aatacccttt tctttaagag
    15421 ctatacaata ctttcttcct tgaaaataag gctctaaaag caccttttca gctttttcag
    15481 gaaatgtctt aggctcatat agcttagcat ataatgcttc tgctttatac tcgtcacttc
    15541 cccataataa cttacttgat ttttttgcat attcaatctt attttgttta gatatttttg
    15601 gattatctaa aaatcgataa aattgtattg gtgaaaatct agactgataa tggtgacttg
    15661 tgactccgtc tttttccata aaccattgca tagaaattaa caagacaact ttcttattat
    15721 caatattagg attcatactt cctaatattg cagcatcttg taatgtctga gtataggctc
    15781 tcccaattgc aaacacctta tttttactcc tatttgtatt aaaatagtac tttggatgtt
    15841 gttttgttga attactaagt tctgatgacc ccaaaatcat aatatccttt tctcttaaaa
    15901 aatgattatt tgctgtaacc cctttatctt ttatatcact taaagtatca tccataattg
    15961 gaagtagatt tttctccctc aaaagttcat cagttttaga atcaagaaat ttgtctaacc
    16021 caaataaaaa tactacgcct attataaaag gtgtaataaa gtatatcaat tttctcatat
    16081 tttctctcct ctaaaaatat tcaaatttat gttattatta atcatattag ttttgatatg
    16141 tatattcaac tacaaaattg taataaaatc gtcaaaactt tttttgatac tgttacaaaa
    16201 ttgtaaaaaa aaagaaaaaa acgccaaaaa taaatttctt tttagcactt tttcatactt
    16261 ataatttgac attttaacac ttggttaagt atacacctta ccttaactta aaattaaatt
    16321 tatttaagaa tatatattat aaattcttat attttctaat ataaactata tactatcagc
    16381 tggataaaca atactacttt gtcttcttat ctcatccata atttccatta ctattattga
    16441 attttcatga gaatttatat ttgattctat tttattgtta tttataagac ttataaactc
    16501 tacaagttca taatacattc cttctatttc tctaggcttt tcctctgctt tttcttttgg
    16561 aatagatata tcttcttctc ttccatctct gtatttaata atagcttttt caaatagatt
    16621 aagcttttca attattatac ttccctcttc tccctgtatt tcagatggta tataagaatc
    16681 agctacttta gaatactgaa ttatcgcatc catatcatca tattgaaata cagctgaacc
    16741 ttctccatcc actccagttt gaagtatatg agaaactgcc tttacagttt ttggagcacc
    16801 aaataaattc accataggat gtatacaata cactcctata tccattaaag ctccatttga
    16861 caactctttt ttaaaggcat tttctataat tccattttta tatttatcat atcttgaaga
    16921 atactgacaa tagcttccaa aaaatcttct tattttccct attttataca agttttcttt
    16981 aactgcttta aagtttggaa cacatgtaat tctcatagct tccataagca atacattatt
    17041 atctttggct gtttttatca ttgatttgac ttcttttaaa ttagatgcta acgacttttc
    17101 acaaagcaca tgttttttgt gttttaaaca agtaatggtt tgctttgaat gtaatgcatt
    17161 tggagaagct atgtatacag catctatatc cttcgattta gccatttgtt ctaaatcatc
    17221 aaaaaaaata cttgctccat atagagttcc aaattctcta gctttttcta aatttctaga
    17281 atatacagca accaattcaa agtctttaac cctagatgca gctcttagaa atatatttgc
    17341 tatattgctt gtcccaacaa taccaaatct tattttaccc atcataaatc actccccatt
    17401 ttctacactt tattttaatt ttaataaaaa actttacaac ttatagatat attataaaat
    17461 aattttatca aataaaaaag aaagctaatt ttaaatgatt tcaccatttt aacatagctt
    17521 tcttttaact acaatctaaa ttcaacttat atttttaaag ttatctttct actatatctt
    17581 tatttcccct caaaaatttt atagtaagaa tttcatccac aaacaatgtt actaaaagta
    17641 tagttacaac aattggccaa taaggtatta taagactata taaactatca actatataca
    17701 cttgaaaata tctaactcca ataaatgtca atatagtcca atatacagaa aactttagac
    17761 atactattcc tctactattg tacttataat ctgaataatc ccaatagacc ttattaaaat
    17821 atactttcat aagtattcct gtaatatatt ctactactgt cggcactact agacataata
    17881 aaagcaatgc aaatatatta ttggggtcaa taaatcttga gacctctatt ataatcgaca
    17941 ttgcaattgc atacattggt ttaaatggac catttaaaaa tccatcttct tgaaaatgcc
    18001 cttgtttata ataactatat aagttttcaa ttatccaacc tataaaacca tacaagaaaa
    18061 aattaaataa aaaatataat agagaaccta cattatccat gaatattcct cctagctgtt
    18121 tatacttatt ttttctcata ttcatggatt tattctaaat caaagaaatt atatttaatt
    18181 tatttaatta atactttata ttatagattg tgacattctt tagagttcaa actttttagg
    18241 tattctctaa cttccagtaa agtttcaagt atcttatgtg attgtgattt aatttgctca
    18301 tctggctctt ttaaatctgg tacatttata catcttatac caccattgta tgcagcttcg
    18361 actcccatag gcgaatcctc tatgacaata caatttttgg gattaacatt tatcttttta
    18421 gctgctttta ggaatatctc agggtttggt ttagagttta caacatcatc tccacaaact
    18481 atagcatcaa agtaatcttt taaatttgcc ttagctaatc tctttactgc tctttctctc
    18541 tttgttgatg ttgcaacagc cattttgtat ccattttctt ttaaaaaaga gattagttca
    18601 ttgactccta atttgatagg agctcccttc ctctccataa attcaatcat gtttttagtt
    18661 ttttcgtcat ataaatctat tattggtaca gaactatcat atatctctgt aagaccttct
    18721 attattcctt tacgatttct tcccataact gatgtataaa tttccttagt catagtatat
    18781 ccatactttt caaacgtctc catccaaaat tcaagagaaa ttctctctga gtcaaacaaa
    18841 actccatcca tgtcaaaaat aattccttct actttttgca tacaattcct ccaatatttt
    18901 ttgatataat tatattacac tttttatgat tagttttaca accctcaatt tatccaattc
    18961 taatattgta aaattgtaat tttcaaaagt tacactttgg tttactttaa tatttgaacc
    19021 aagcatatag gatatccacc cacctatact gtcaaatcca tcatgctcta tatcaagtcc
    19081 aaaatactta ttaatatagt tgatagattc agttccatca actaagtaag aacctgctcc
    19141 tattttctta atatgagtct cttctttgtc aaattcatct tgaatttctc caactatctc
    19201 ttctagtata tcttctattg ttaccacacc agatgtacca ccatattcat ccacaactat
    19261 agcaagttga agtttttcct ttcttattct ctcaagagct ttatctatag ttaaattttc
    19321 agatatatat ataatatctc tcaatatttc ttctagttca attttatttt catttatctt
    19381 ttgattgtat aaatctctta tatgtacaaa acctaatata tcatctttat tttttctgca
    19441 tactggatat cttgtaacac cttcctcttt taaaatagct aatatctttt cttcagaatc
    19501 actttcatat atacatacca tatcagttct aggaaccatt atctctctaa ttttcttttc
    19561 ttcaaattca aaaatattat caactaatct ttgctctgat tcatctataa gtccactttt
    19621 ataactttct tctactaata gtcttatttc gtcacctgta tggggttcat caacctcatc
    19681 agcttgtgaa tatccaaatg gtttaagaaa taaatcagta cttaagttga aaaaatatat
    19741 tattggatat gttaacttat aaaatcctac taataaaaaa gatgtactca acattatttt
    19801 ttctgtatta taaagtgcta aagctttagg aaccaactct cctattacaa cctccatcat
    19861 tgttataatt aaaaaagaaa tggaaattga tatagtatat ataatacttt cactaagatt
    19921 aaatacactt actattggta aaattaactc tttaactgta gattctccca tccaacctaa
    19981 tgcaagtgaa caaagtgtta taccaagttg acatgcagaa agataggaat ttaagttgtc
    20041 ttttattatt ttacatcttt ttgcattatt attaccttca gttatcatgg tatcaatcct
    20101 agaactacga agttttacca tagcaaattc acaagctaca aaaaaagcat ttatcaaaac
    20161 aagcattact ataagtgtta cttttaataa ctcccgcata aagtataggt tcttataatg
    20221 taagaaatct ataagaactc aaagttcccc ccttatatta ataaatttaa tttataatat
    20281 atattctatc atatttatta atatatccta ctttattaca aataattttt ataaactaaa
    20341 taaccccttt taatatttat gactggctat ttgataaata ttaaaggggt tatttagtta
    20401 tcatttttta tttctaaaaa tcttttctca aatgatttag ttatc
//
LOCUS       DAMJYC010000068        20295 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJYC010000068 DAMJYC010000000
VERSION     DAMJYC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.069"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            207..2066
                     /locus_tag="PC472_002683"
     CDS             207..2066
                     /locus_tag="PC472_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728000.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2340..2939)
                     /locus_tag="PC472_002684"
     CDS             complement(2340..2939)
                     /locus_tag="PC472_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2728001.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3042..3839)
                     /locus_tag="PC472_002685"
     CDS             complement(3042..3839)
                     /locus_tag="PC472_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2728002.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3952..4057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4149..5135)
                     /locus_tag="PC472_002686"
     CDS             complement(4149..5135)
                     /locus_tag="PC472_002686"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2728003.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5619..7349
                     /locus_tag="PC472_002687"
     CDS             5619..7349
                     /locus_tag="PC472_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2728004.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7459..7539
                     /locus_tag="PC472_002688"
     tRNA            7459..7539
                     /locus_tag="PC472_002688"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7493..7495,aa:Leu,seq:tag)
     gene            7874..8389
                     /locus_tag="PC472_002689"
     CDS             7874..8389
                     /locus_tag="PC472_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2728005.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8881..8985
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9195..11669
                     /gene="gcvPA"
                     /locus_tag="PC472_002690"
     CDS             9195..11669
                     /gene="gcvPA"
                     /locus_tag="PC472_002690"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2728006.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11669..13126
                     /gene="gcvPB"
                     /locus_tag="PC472_002691"
     CDS             11669..13126
                     /gene="gcvPB"
                     /locus_tag="PC472_002691"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2728007.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13549..15906
                     /locus_tag="PC472_002692"
     CDS             13549..15906
                     /locus_tag="PC472_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2728008.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15992..16309)
                     /locus_tag="PC472_002693"
     CDS             complement(15992..16309)
                     /locus_tag="PC472_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2728009.1"
                     /translation="MSEFKNITAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(16893..18164)
                     /locus_tag="PC472_002694"
     CDS             complement(16893..18164)
                     /locus_tag="PC472_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2728010.1"
                     /translation="MLKVLKGNIIFAKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   18811..20145
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18811..18839
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         7593 a         2373 c         3189 g         7140 t
ORIGIN      
        1 attaatagta ataatctata agaacttata tattagaaaa aaatttttgt atatatagag
       61 gactctaaag atgaactatg taaaacaagg tttatatatt ctaatagtat agaaaaattt
      121 attgtaaacg catgtatatt attagaaaaa atagtttgaa ggtattcaat aaacataaaa
      181 aacaatatag gggataaagg tgttttatga agagattttt gaggagaatt atcttagttt
      241 tatttatttt gttgattttt ataagtttta tttctattaa attaattcat aatgttggag
      301 attatggaaa attgattaat tatgttggga ttgttcgtgg agctagccaa cgtctaacta
      361 aactagagat gaatcatcaa ccaaatgatg aattaataga atatattgat gaaatcctac
      421 aggagttgat tacagggcat ggagattatg gtttagtaat cactgattgt aatgaataca
      481 atgaggactt acttttatta gaaaaaaagt gggaagattt aaatattgaa attaaaaaag
      541 taagaatgaa agaacagaat aaccagttat taagtataag tgaagagttt ttttctcttg
      601 caaatgatac agtgtttaaa atagaaaact tttctaaaga aaaaagtaat tacttaatga
      661 ctttaataat aattatatct attataggta tattagcatg tatcatattg attttacaat
      721 atagtaaaaa aatggttaga ctggaaaaat taaatgtaga tttaaaaaat attgcatata
      781 aagatgaact aacaggagta aatactatag aaaaatttaa attggatgca aatcaaaata
      841 tttgtataca taaggataag aaatttgctg tattctatat tgattttgaa aactttaaat
      901 acattaatga tatatttgga tatgattatg gtgacatgat actgaaaaga tatgctaatc
      961 ttatgatgaa tgacataggg aaatatgaaa tatttgcaag agaaattgca gatagatttg
     1021 ttgcgttaag atgctatgta gataaggaag atttagtggt aagacaacga gatgtagaca
     1081 atgaactaat taatataaca aatgaaatta aaaataaaca tagtattact gttgttagtg
     1141 gaatttgttg tattgaagat gtaaatgaaa aattaagtat agatggttta ataaatagag
     1201 caaattttgc acaaaaaact gttaagaata aaccaggtac taattatgct ttttataatg
     1261 acagtattcg taaaaaaatg attgaagaaa atactatcaa gagtagaata catgaagcta
     1321 ttgaaaaaag agaatttata gtttatttac agcctaaagt taatttacat agtcaaaaaa
     1381 taaattgtgc agaagcttta gttcgttggc ttactccgga taaaggtatt atatctccag
     1441 caatatttat tccagtactt gagaagaatt tttttattgc cttagtagat aagtatgttt
     1501 ttgaggaggt ttgtaaatgg attagaaaga ggttagatga aaataaacct tttgttcaga
     1561 tatcagttaa tgtttcaaga atacaatttt ataacactaa atttgtagaa acttattcca
     1621 atatacaaaa taaatacagg atacctaaaa atactataga aattgaattt acagagagtg
     1681 ttgcttttga gaatcaaaaa catcttttgg aaattataca tgatttgcat gaaaatggat
     1741 ttacttgttc tttagatgat tttggaaaag gttattcgtc tttaagtgtt ttaaaagatt
     1801 taccatttga tgttttaaag cttgattcta tgtttttcaa ggctagttta gataaagata
     1861 aagagaaaat agtaataaaa aatattgtac atatgttaaa agaacttaat atcacaactg
     1921 ttgctgaggg cattgaatgc aaggaacaag ttgaatttct tagggatatt gggtgtgatt
     1981 tagttcaggg atttgtattt tataaaccaa tgccaatatt agaatttgaa aaaatattgg
     2041 ataaagagtt tgtttataat agctaaaatg tactttataa aatatgaact aattaagtaa
     2101 aattattttg tactgaataa atttttactt tatttacaga aaagcgcgaa aagtgtattc
     2161 ctttaagtgt gggatggata gcacaaatag aggagaaaaa atattggcca tataagaatg
     2221 cataataata taaatatttg aaaggtcagc attatataaa aattaaaaag aaaataaata
     2281 atttttctat aaaaaagaga ctatgagaaa atttactcat agtctcctaa ataatgtatt
     2341 tattttcttc ctgcaaccca ttttaatccc caatgaaatt cagtatccat ttctctatga
     2401 cctttgaagt atttaactac tttttcaaca ctgaatgtct catcatttac attttctatc
     2461 aatgctattg aacacatatt atagccattt ttatgttcat caagtttgac aactaagtct
     2521 gaatcttgtt tttgtttgat tgttacaatt ccatcagctt cagaccagtt agctacacct
     2581 tcgtatataa aagcataaat aagtattctt tttatatctt ttattttatt accatttatt
     2641 cttaaatttt ctccttgtgt attacttcct gttctgtcat ctccatctaa ttgagcaaaa
     2701 ggtggattgt ctaaactacc aaaggcattt cctaatgctt gaacagctcc ttttacacca
     2761 tttttcatct caaataggca acccaaatct aaatctatat tactgcttct taaagagcca
     2821 aaaaaaccct ttttttgtac tttttgattc caatttaaat taactaagat ttcacctaaa
     2881 tcgctatttt ccttttttgt aaggtttatt ttttgtccct tttttaattc tatagccata
     2941 gtaatcttcc tccacaaaca tagatttatt taaatggtag cacttaataa gtcaatattc
     3001 aacaagatta gaataataat attaaaatta tactaaatat actaaaaagt aggaatcaca
     3061 atacctttgt atttttcttc aattaagctt cttgctttat ctgatgtaag aacatcttgt
     3121 agaactttaa tcttttcact gtctttatct tcagttctaa atgctaaaag atttgcatac
     3181 tcgtcattta taggagctgt ataaattccg tttttcttgg catcaagacc agcaggtaca
     3241 gcataagtag tatctataaa tgcagcagtc acatctggaa gagaagatgg aatttgagca
     3301 atatctaact ctttgaattg tattcccttt ggattttcta ttatgtcctt aggactaact
     3361 aatccatccc ctttaggtaa cgttaaaagt ccaatacttt caaggtatct aaggttctta
     3421 gaacaagaag atgggttatt gcttattgca attgtatctc cttttttaaa ttcatcaaca
     3481 gattttatct tataagagta aagtattgct gggcatacat atagtttttt accagccatt
     3541 attttgtaac ctttggcctt aacagcttct tttagataag gttcatgttg aaatagattt
     3601 gcatcaattt caccattatt taaagcttcg tttggaagta tgtaatcatc aaagttttta
     3661 acttctaaag tatatccttt ttctttaata agtggtttta attcttctaa taactctcct
     3721 cctggaacta aagtagcacc aacaacaatt tttttatcat ctttagcttt agaacatcca
     3781 accacagcta aagttaatac tagacataat aacaaacata ataatttttt gaatttcata
     3841 taaattccct cctgaatata attgattagt taaaaataaa ataccattct ttatcacact
     3901 gcaaaataaa aataagcctg agtttaaact caggctttaa aagctaattt agactcatct
     3961 ttcagtataa tactgcagga tttagcacca atgtatataa aaatatactg gttgctgggt
     4021 ttcatagggc cagtcccttc accactctgg ataagaagta tcaattttta attatgttat
     4081 aaatattatc atgatttttt acgtctgtaa acataatttt ataaatattt taaaatcttt
     4141 aatgtatttt atttaacacc taaaattcca gaaactagaa tatcagtatc agcacctaag
     4201 aaatagtcat ttatttcaat agaatccaga gtatttctca aagcattttc ttcatgcctt
     4261 ataccaataa gtttattttc tacaaatgca acatcacatt ttccaaaaaa atctccaaaa
     4321 aattttatac tagttattat gcctttatca acatttaaat taaactcaac atttccacca
     4381 gaatatttta attcatttga aagagtatat tttggtgaat taccaaaatt ccaatcccaa
     4441 gtttcgtatt ttgatttagt aagcttttct atttgtttta tatcatgctc actcaaacaa
     4501 taattagtat tatcagtatt tgttttagaa agataatcca ttatagcatc tttaaattct
     4561 aatatgtcca tttttgaatg taaatgttca cttatattgg ttacacgaga ttttacagat
     4621 tttattcctt ttccttcaaa ttttataggt ttaaccttta atgcttttga gaggtcattt
     4681 atttcagatg aaaataaaag agtaccatga tgcattactt tgtttttgta gttgtattga
     4741 gcatttccag aaaatttctt gccatctata agaaggtcat ttcttccaga aaattcggca
     4801 ttgacatcta gtgttttaag taaatctatt atgggctgag tgaatcttct gaaatcacta
     4861 aaattattat tgttacaagc tataaatgta aaatttatat ttcccaaatc atgaaataca
     4921 gctccaccac cagactgtct cctaacgaca ggtatagaat attcttttat atattctaaa
     4981 tttatttcag aaagtgtgtt ttggtttttt ccaacaatta tagaaggttc attcctccat
     5041 aaaataaata agtcttcatc aaaatttttt agaagatatt cttccattgc taaattaaaa
     5101 taagggtttg ttttttcatt ataaattaat aacattaaag tagttcctcc atagaattga
     5161 taaaattttt attatgaata caattaattt tataattcga caataatata aaaactcctt
     5221 taaattagaa attttaaaaa ctaccttgca aagtttaaat aaataatata tactcaaaat
     5281 atgtttaata aaagtgaata gaagggtaga ggcgcaataa tctatcagta ctaataagga
     5341 gttaagcaca gagatttatt aggaaaggag atattgccga agttttataa tcgatgcttt
     5401 aagattatat tgctgggggt acatagaaaa tatgtacaac tgtcacaggt ttatttgtgg
     5461 tgagctatca tttaaggaat tatattatat ttatttttac tattgttgat atattgtaat
     5521 gtttaagcct tgagtgttta ccactcaagg cttttttagt tttattgtaa cataataccc
     5581 tgtacagttc acaaaattaa aaggagaggg agatttatat gaggaacaaa aaaattaata
     5641 ttatttttct gacgacaatc atgttcataa tgtcaacagt aatggttttt gcagaagaag
     5701 atatagacac tattgcacta gccaatgcag aaaaatttgg gattttaact ttaataccac
     5761 cattagttgc tataatactt gcatttataa ctaaaaacgt aataatttct ttacttatag
     5821 gtattctttc tggtagtttt ataattaaag ctagtggtat taatgttttt gcgacattta
     5881 tacaggcatt tttagattta gtagacagag ctttagtatc tttagcagac ccttggaatg
     5941 cagggataat actacaggtt ttggcaattg gaggagtaat aaatcttgtt gctaaaatgg
     6001 gaggagctaa agctatagct gaagcacttg ctaaaagagc aaagtcagca aaagggacac
     6061 agcttataac atggtttttg gggcttttag tattctttga tgattatgcc aattcactaa
     6121 tagtaggtcc tatgatgaga cctgttgcag ataaaatgaa aatatcgaga gaaaagctag
     6181 catttataat agatgccaca gcagcaccag ttgctggttt agctataata tctacatgga
     6241 taggtcttga ggttggttta atacatgatg cttttgaaag tataagtata gatgtagatg
     6301 catttggtat atttttaaat acaataccat ttagatttta caatatatta atattagctt
     6361 tcattgtaat atcagcatta ttattaaaag aatttggtcc aatgagaaaa gctgagataa
     6421 aatctagaag tagaaaaatt agtatagacc ttgatgaagg cgtagaagag ttggatgatt
     6481 tagctccaaa aaatggagta aaattgagtg tatggaatgc tataatccca ataggaacat
     6541 tgataatagt tgcattagca tcattttatt atagtggata tacatctata atgggaggag
     6601 acgataaggc tctgatacaa ctatttacaa attctccata ttcttttgaa gcaataaaag
     6661 aagcatttag tgcatctgat gcatctagag cattattcca gtctgcactt gttgcaagtt
     6721 tagttgctat tataatggct gtagtaaaaa agatttttac aatatcagaa gcaattgatg
     6781 tttggataga tggaatgaaa agtttagtaa tcactggtgt aatattaata cttgcatggt
     6841 cattaagttc agtaataaaa gagttaggta cagctaaatt tttaatacac ctattatcag
     6901 gttcactacc accattctta ttaccaagtt taatatttgg tttaggagca ataatatctt
     6961 ttgctactgg aacagcttat ggaacaatgg gtatacttat gccacttgca attccattag
     7021 catactcatt aaatcctgat atgtcttatg taatagtaag tactagtgca gttttaacag
     7081 gtgcaatatt tggagaccat tgttctccaa tttcagatac aacaatactt tcttcaatgg
     7141 gagctggctg taatcatata gaccatgtta acactcaaat gccatatgct atatttacag
     7201 cagtaatcac tattgtattt gggtatatac cagctggact tggattgcca atatatatag
     7261 tattaccagt ggcaatagct gctatatttg taggtattca aataattggt aagaaagttg
     7321 atgaagcgga aatagaactt gtagaataaa gatataaata agtttcacct tttattatca
     7381 ctattttgtt agtttttaga taattttaaa aagttattga aaataatggc gaaatatagt
     7441 aaaataaaag agtgataagc ggatgtggcg gaattggcag acgcactaga cttaggatct
     7501 agcgccattg gcgtgggggt tcgactccct tcatccgcac tttacagtac ttaattgtac
     7561 tgaaaaaaac tggggttgct ggctttcgga gtgggctatc ccgtttttat ttttttgtat
     7621 atataataga ttggcacttt agtataaggt gcttttttta taggcaaaaa cagtacattt
     7681 atttctgttt tattatcatt catctattag aatatacttt agttaagaat gtgttaataa
     7741 gcagtgttca cattcttgat aaaatgtgga ataatatgta tttttactaa gacggaaatg
     7801 aaataattaa accattaaat aatattataa gatgatgaat aagtagataa atcaaatttt
     7861 gtaggaggat attatggaaa aatatatatg gttgtttcca ttacttttta tttttcatga
     7921 tatggaagag ataataggtt ttggaatatg gcttaaaaaa aataaatcaa tgcttgataa
     7981 gaagtatcca tttatcagta aagtttatga aaattatagt actgaaggca tggcatttgc
     8041 tgtgtttgaa gaatttattt tatgcattat tttctgtata cttacagtta taacagagaa
     8101 tcagtatgta tatctactat ggttaggtag ttttatagca tataccttac atttggtaat
     8161 tcatataggg cagtcaatca taataagaaa atatatacct tcacttatta ccagtatcat
     8221 atgcttacca ataagcattt ggtgtataag taagagtatt tatatagttg attgtgaaat
     8281 gagtacaaca attctgtaca gtataattgg cattataatt gtagcattga atttaaagtt
     8341 tgcacagtcg ctgattggaa aatttacaaa gtggatgtca aacatataaa ttttagttta
     8401 ttggacagtt caaggaatca gaaattggaa ttttatagta ttaatatttt atggtattaa
     8461 tataatgtat aaaattttat agcaatgtat ttcataagta taaagtgact agaattttaa
     8521 gaaatataca aattctataa aaactaaata atttcaagta tattttaaaa atcttatttt
     8581 tcacaagttt aaaattgtaa acgatttcat caagtctatg aatatacttt taacatgtta
     8641 tagtaaatct caatataaat ttacatttgg ttctttgtgt gttattttaa taataaatta
     8701 agtgaaaatg ttattttaca ttaatatata atatctttgc ataagagtga ataatataaa
     8761 agagaaataa ttaataataa aaacttaaca aaaaaattaa tacataaaaa aaaataaata
     8821 taatgtgttg acatttaatt gaaatattca tacaataaaa ttgaaaataa atatttccaa
     8881 atgagattag cgagagagtc ctttatacga aaaggcgccg aagaagaaat ttaagaaatg
     8941 ccaattttta gagaagcttt caggcaaaaa tatcgctaat tgatgggact ctggagagac
     9001 ttaagttaag atactataag tcaccgaaga agcaaaataa tattaaatta ttaaatcttt
     9061 caggtataca aacagggata tataggctag gattattttc catgctgata tatctttttt
     9121 ttatgaaaaa gtacaggaaa taatatcaaa gttacttaaa tacagtaggt ctaattttct
     9181 agggagggtt atatatgaat gaattgaaaa gagtatcttt gtacaatata cacaaagagc
     9241 ttggagcaaa acttgttgaa tttgctggtt gggaaatgcc tttagaatat gagggtataa
     9301 ataaagagca tgagaaggtc agaaaaagtg caggtatatt tgatgtatct catatgggag
     9361 aggtacagat aaagggagca gagtcagaaa aatttataca aaacctagtt acaaatgata
     9421 taagcgcatt aaagataaat gatattatat atacacctat gtgttatgaa aatggtggag
     9481 tagttgatga tttattaata tataaatttg gtgaagaaga ttatttatta gtaataaatg
     9541 caggtaatat agataaagat gtagcatgga taataaaaca aagtgaagga tacaatgtag
     9601 atataaaaaa tatatctagt gaagtgagtc aattagcaat tcaaggacct aaagcagaag
     9661 aaattcttca aaaaatcaca gatatagact taaactcaat aaaattttat aagtcaattc
     9721 catcaacaaa agtatgtggt tgtccttgtt tagtttcaag aacaggatat actggggaag
     9781 atgggtttga gatatactgt aaaaataaat atgtagaaat aatttggaat gaagttctaa
     9841 aggttggagg agaagatata tgtccagctg gtctaggttg tagggatact ttgagatttg
     9901 aagcagctct acctctatat ggacatgaaa taaatgaaca catatctcca attgaaggtg
     9961 gtttatctat ctttgtgaaa acaaataaag aatcatttat tggcaaatca attttatcaa
    10021 aagaaaaaga aagtggagct aaaagaaagt tagtaggatt tgaaatgcaa ggtaaaggaa
    10081 tgcctagaaa tggatatgat ataaggattg gtgataaaac agttggattt gtaacaacag
    10141 gatgtgcatc tccaacgaca ggaaaaattt taggtatggg aattatagat tctgaatatg
    10201 caaaagtagg aaatgaaata ggtatagcaa tcagaaaaaa agttgttcct gctgtgatag
    10261 ttaagaaacc tttttataaa aaacaatata aaaaagataa tattatatta aacaaagaaa
    10321 ataaattttc atatatacct gctacaagtg aagataaatc aaaaatgtta aaagtagttg
    10381 gattaaattc ggttgatgaa ttattttcag atataccaga agaagtaaaa ttaaaaagag
    10441 atttaaattt ggaaattgga aaatctgaat tagaggtaag caaaatagta aaaagattgt
    10501 cagaagaaaa tttaagttta gaagacttga cttgttttct tggtgctggt gcatatgacc
    10561 attatatacc atcaattata aaacatataa cttcaagatc tgaattttat acagcatata
    10621 ctccatatca agctgaaata agtcagggaa ctttacaagt agtttttgaa tttcaatcta
    10681 tgattgcaga aattacaggt atggaaattg caaatgcatc aatgtatgat ggagcaactg
    10741 ctgcaataga agcatgtata atggcaatga atcagactag aaaaagtaaa atagtagtgt
    10801 caaaaactat tcatcctgaa actttgtcaa tattaagaac ttacttacaa tataaagatt
    10861 gtgagatagt agaaattgac ttttgtaatg aatatggcac tacagatata gaaaaattaa
    10921 aagcttctgt agacaaagat acagcatgtg ttcttataca aactcctaat ttctttggaa
    10981 ttattgaaga aatggaagaa atagaaaaga taactcatga aaataaggct atgttaatta
    11041 tgagtgtaga cccaatatca ttaggagttt taaaaacacc aggtgagata ggtgcagata
    11101 ttgttgttgg agaagctcaa tcacttggaa accctcttaa ttttggaggt ccttatgtag
    11161 gatttttagc tagcaaatct aaatatacta gaaaaatgcc aggaagaata gttgggcaaa
    11221 gtttagatgt agaaggtaaa attgcatatg tattaacttt acaaactaga gaacagcatg
    11281 taagaagaga aaaagcaact tctaatatat gttcaaatca agctttaaat gctcttgtag
    11341 cttcaatata tatagctact atgggtaaag aaggatttaa agaggttggt atgcagtcta
    11401 tgaaaaaagc acattatact tacaataagc ttgtacaaac tggaaaatat aagccaatat
    11461 ttaaaggtaa gtttttcaaa gaatttgctg tacaaggaaa tcttaatata gaaactataa
    11521 atgataaact cttagaagaa aatatattag gtggttataa tttagaatac aattatccag
    11581 aattaaaaaa ttcaactttg ctttgtgtga ctgagaaaag aagcaaagag gaaatagata
    11641 agttagttgg aataatggag ggattataat gaaagaatac aatagtttgt tgatagatat
    11701 atctaaaaaa ggtaggaaag catactcact tccaaagtta gatatagatg atataaaaat
    11761 agaaaatatg attgaccaag atatggcaag acaaagtgag ttgaatttgc cagaagttgg
    11821 tgaattagaa ttagttagac attatacatt attatcaaat aaaaactttg gagttgatac
    11881 aggtttttat cctttgggtt cttgtactat gaaatataat cctaaaataa atgaagatat
    11941 ggcagcactt ccaaacttta ctggaatgca tccataccaa tcttcggata cagctcaagg
    12001 ttctctttct ttgatgtatg atttatcaag aagacttgca gaaattacag gtatggatga
    12061 agttacacta caaccttcag caggttctca tggtgagttt acagggctta tgattataaa
    12121 agcatatcat gaaaatagag gtgaccataa gagaaccaaa gtgataattc cagattctgc
    12181 acatggaaca aatccagcaa gtgcagctat ggcaaacttt gatgtgattc aaatagcatc
    12241 tgataaaaat ggagctgtag acataaatgc attaaaagag gttttgaatg atgaagtagc
    12301 agctcttatg cttactaatc caagtactct tggtttattt gaaaaaaata taaaagaaat
    12361 agctactctt gtacatgaag caggaggact tttgtactat gatggtgcaa acatgaacgc
    12421 tattatgggg ataacaagac ctggagatat ggggtttgat gtggttcatc ttaatcttca
    12481 taagactttt tcaactcctc atggaggtgg aggtccagga gctggtcctg ttggagttaa
    12541 aaaagaatta gtaccttttt taccaatacc agtaattgaa agaaaagaag ataagtatgt
    12601 actaaattat gatagagaaa aatctattgg gaaaattaaa aatttctatg gtaattttgg
    12661 agttcttgta agagcttaca catatatact aactatggga agggatggac ttaaagaggc
    12721 tagtgaaatg gcagttttaa atgctaatta tataaaggaa agtataaaag atgattatat
    12781 tttgccaata gataccttgt gtaagcatga gtttgtatta ggtggattgc caagaggaga
    12841 ggccaatata aaaactatcg atattgcaaa aagactacta gattatggat accatcctcc
    12901 aacaatgtac tttcctctta ttataaatga ggcacttatg atagaaccta cagaaagtga
    12961 gagtatagag acattagata gttttataga agctatgaaa agtgttgcta aagaggcaaa
    13021 ggaagaacca gaactattga aaactgctcc acataatact ttggtcaaaa gagtagatga
    13081 tgcaagggca gtaaagaaac ctatactaac atggtcacaa agataaatag ttaaaaagta
    13141 gagatttaaa gtataaagag aattaaattt gtaagaaaaa gctatctaac ttaatctagt
    13201 ttagatagct tttttgtata ttagtatgta tggtattttc tatataaagt tttaaattat
    13261 attagtgctt atatagaata ttgatatgat gattatgtat agatattaat aagaattgtt
    13321 gaatttatct tgccttaact ttatttactc ttttatatta agaaaaactt aactaaaata
    13381 tagcgaaatt cataaaaatt taggaaatct taagaattat aatatttaaa tctttagatt
    13441 tatattttat tatataaact atagaataat aaaataaaaa aattgagaat tttataatat
    13501 atttgtagat ttgagtataa aaaagtgcta agttgaaagg agtaaaatat ggataaggtt
    13561 aatttagaaa ggtatagttg tgatataaat gttggtttaa ataaaaatca acttcaaagt
    13621 agaatagatg ataatttaat taatgcattt gataatggaa aaaccaaaac atataaacaa
    13681 atttttaaag acaatatatt tacacttttt aatctcataa atattgtttt agtctgttta
    13741 ttagtttcag ttggttcttt taaaaatacc ttatttatat ttattgctgt aattaatact
    13801 attataggag taatacaaga gataagagct aaaagattat tggacaatct atctgttata
    13861 gtttcaaata aggtatctgt cataagagaa aaagagaaaa aagagattga tgtacaagaa
    13921 ttagtattag atgatattat gattttaaaa acaggtaatc aaatttgtgc agattcaaaa
    13981 gttttaaatg gaaaattaga ggtaaatgag tctttggtta caggtgaatc tgatattatt
    14041 ataaaaaata aaggcgattt tttatattct ggaagttttg tagtttctgg agaagctttt
    14101 gtaagagttg aaaatatagg aaaagataac tatgcaaata agatagctaa tgatgcaaag
    14161 atttctaaaa aacataattc tcaacttaga aattcattaa atgcaatatt aaaaattgta
    14221 ggtataataa taattccttt aggaattata ttgtttgcaa aacaacattt tggaaatgga
    14281 gattctatag ctacatctgt agtaggtaca gtggcagctg taaatggtat gataccagag
    14341 ggattaacat tacttacgag cattgctcta gctgtaggaa ctataaaact tgctagtcat
    14401 aaaactttag tgcaagaact gtactgtgta gagactcttg ctagagttga tacactatgt
    14461 cttgataaga caggtacaat tacagaaggt aaaatgcaag ttgatgatat agtaatgttg
    14521 gaagaagtgg atattaatga aataatgggg aatatatgta attctctcaa agatgataat
    14581 gcaacactaa atgcaataag agataagtat aatgttttag atacgtataa agctaagaat
    14641 ttaataccat tttcttcaga aagaaaatac agtggggtta cctttgaaaa taaaggtaca
    14701 tatttattag gtgcatttga atttatattt aaggaaaaaa ataaaaaact taggatagaa
    14761 gttgaaagat attctaaaaa aggaaacaga gtaatagttt tagctcatag tgattcttat
    14821 atggaagata ataatattcc tgaagatata aagccactag cttttatatt gatattggat
    14881 aaaattagag atgaagctaa ggaaacttta gaattttttt acaatgaagg tgtagatata
    14941 aaaattatat ctggtgacaa tcctataaca gtaagtgagg tagctagaaa agcaggtctt
    15001 aaaacagcta gtaattttat tgatgcaaca acacttaaaa atgatagtga tatatacaaa
    15061 gcagttgata aatacagtgt ttttggaagg gtaagcccac aacagaaaaa acaaatgatt
    15121 ttagcattaa aaaaacaaaa tcatacagta gctatgactg gagatggtgt aaatgatgtg
    15181 ctagcactta aagaagcgga ttgtagcatt gccatggcat ctggaagtga tgttactaag
    15241 aatgtgtcta atttagtctt attagattca aactttgcat caatgccaaa ggttgttatg
    15301 gagggaagaa aagtaattaa taacattcaa agagcatctt ctttgttttt agtaaagaca
    15361 attttttcat tcttcttgtc gttattaaca ttattcttct tcagtagaca gtatccattt
    15421 gttcctatac aattatcact cattggggct gtaacagtgg gtataccatc attcttctta
    15481 gctcttgaag caaacaaaag tagaatttca ggaaattttt tactcaatat attaaaaaat
    15541 gctttaccag gagcactatg tgtgacagta aatgtaatta ttgtttacaa tatagttgaa
    15601 tatttaggtt atagttcaag tgtattttcc acaatgtgtg ctatattaac tggtgtatgt
    15661 ggtcttttaa tattaagaca acaatgtttg ccatttaata aagaaagatt atttttaata
    15721 gtaagtatga ctattgcatt tgttattggg attctatttt taggtggatt attctcattt
    15781 gtagcattga gtagagaatt aattctcata actatggcat tagcaatatt aatgccaatt
    15841 atgataaaag ttatgttatt tgtcttagat gagatattag agacaattgt tccagattta
    15901 aattaatttt aaaatgatat aaataagagc agttttagtt gaactgctct ttttagatgt
    15961 gtttaatttt atgtaagaat atgtattttt attaatcagc tatatatgag caacagtagt
    16021 caacaacttc attaaccttt aagtcaaaac ttgaatcact aggaacatta aatttttgcc
    16081 cttctttata agttacaaac tcattgcttc caggcagttt tacatccata cttccagcaa
    16141 gcatctccat tatttcttct tctctagtag aaaaagtgta ttctcctggc aacattaatc
    16201 ctaaagtttt tctttctcca ttaggcagta ttattactct gctacttacc ttaccatcaa
    16261 aataaacgtt tgccttcttt actgcagtaa tattcttaaa ttcactcatt agataaaacc
    16321 tccaaaatta tttgtacata tatattatat aaaaataatg taaaaaatgc tatatattta
    16381 caaaaaaatt tagtatttat taaaaaaatt aataagaata aaaaagagat gaaattgctt
    16441 aaattgataa aaaatataaa ataaattgca aagtaagcaa gaaaagaata agttgaagag
    16501 ttatatatta taaatattaa aaattttagt attaataaaa aagatattac attaatatga
    16561 aaatatagga gattagaagt gtttgataat tacagtacat gttagatata tcattgtaaa
    16621 ttaacatagc tttgaatata ataatgttca gaaggcaata tttttgaggt ctgaatccaa
    16681 taataatata gtcttatgat tgtagatatc aaaaaatgta agttactagg aatacgtttt
    16741 gtagatttat atatgattaa taactaaagc tataaataaa caaaataaaa agagtatgtt
    16801 tcaacctata gcttttagat ttaaaatata ctcttttatt aattatagtt gtataaatta
    16861 accataatat ataattaaaa tttttattat aattatatat taggttcttc aattagtttt
    16921 ccacatacat atctatgaat gatatttcta tcatcaccaa catatataaa tctttcaagt
    16981 ctttcaagta tagaataatt gtcatgattt aaatttgaat catcaataat caatgcatca
    17041 aaatcataac cttcttcaaa gctaccaact tttccaaaga aacttccacc acttttggtt
    17101 gcaatataaa aagcctcaga taaagataag aaattatact tcttaccaga gttaacccaa
    17161 tataatttag ataattgtat tgcagatacc attgctttaa atattgacaa tgtatgtcca
    17221 ccacttatat ctgatccaag tgctaatcta atattattat cgattaattt tctgataggc
    17281 atagcaccac taccaacatt aaagttagaa gctggacagt gaactgccat aacattattt
    17341 tctctcatta aatctatttc atcctgagaa caatgaacac aatgagccat taaagtagga
    17401 gtttgaccaa ataaattata tctattatat gcatctccat agaatttaga ttcaggttct
    17461 agacttctaa cccattcaat ctcatcataa ttttcagata agtgtgattg aataggaata
    17521 ttatatttta aacataaatc tcctaaacct tttaaaagtt cacttgtaca gcttggtata
    17581 aatctaggtg ttattattgg ttttacaata gaatctgtat cactatactt atttataatt
    17641 tcttctgtat cagtaataga atcctgagtg ttttctaata aagtatcagg gcaattcata
    17701 ttcatattta ctttaccaac gtatgctcca agaccaactt ctttgaatat atccataagt
    17761 ttcatagagg cttctttatg tactgttgca aacactgcta ttcttgtagt tccagatttc
    17821 caaagttctt ttacaaattt tttatacatt tttgttgcat attctatatc tttataattt
    17881 gattcttctg gaaatgtata agtttcaagc catggcaaaa gctctttatc catagccatt
    17941 ccaagatttt taaattgaga ggcatgggca tggaggtcat tcataccagg tattataagc
    18001 ttatcagtgt aatctactac ttttaggttt ttgtattcct taggtatctc tttaaatatt
    18061 ccctttactt tacctttttc cagcataata taactatttt caaatactgt gaaagtttct
    18121 gaagtttttg caaaaattat atttcctttt agtactttta acatataggt ctccttagtt
    18181 ttaaatttgt tttaatatat gtaggtaaaa ttttgtttta caaatcaaaa ttttaccaca
    18241 tgtattatat caaatctaaa ctataaatac tattagataa tagcaaatat tagtgaaata
    18301 cagttgttta ctattattat atataactga catttaagtg acatttaaga aaaatataat
    18361 gcctacttac ataaaatgga atgttattta aagagaactt tgattatatt ttcagaagtt
    18421 tttttatcca tatcgtttaa aacaagaaaa tatctattca tagttatttt tatattagta
    18481 tgtcctaatc tttcagagat gatttttata ttagttctag ctagaagaag aactattaga
    18541 atagataata tagtaagtat ttacaaatat gtaggtgttc ttaaattgat aaattattcc
    18601 attttaattt tatagtttga attttatgat ataataaaaa tatataaatt ttgcagtgag
    18661 cgatattttt gataaagtag ggtttaacag ttgcaatgta agggattgag ggtgtatgat
    18721 aaatgttatc aattgcacta ctcatggttc actgcaaatt tgagagagtt gtatgtgtgt
    18781 aagtactgaa aatacttagt ttattttggg gttttatatt aactaagtgg tatgtaaata
    18841 aacctggatt agcttttatc caacattctt ctctgtttta tattaactaa gtggtatgta
    18901 aatgctacta gaccagctac cactaacagt acgaatgagt tttatattaa ctaagtggta
    18961 tgtaaatata gtataaacac tttcaaccgt ccttgaaagt gttgttttat attaactaag
    19021 tggtatgtaa atttacttag aaacaaatat attgttatta acataccaag ttttatatta
    19081 actaagtggt atgtaaattt aagagcatct tctataccta ttttaaagtt tactgggttt
    19141 tatattaact aagtggtatg taaatttgct atctgagtat taatttcttc tatttcttgc
    19201 ctgttttata ttaactaagt ggtatgtaaa tattataata tgtaactata tctaactttt
    19261 atattatcgt tttatattaa ctaagtggta tgtaaatgta tgtcttttaa agttgctaat
    19321 acaactgttg ggtcgtttta tattaactaa gtggtatgta aattgaatat cactacacca
    19381 aatttttaga cattcaaacg ttttatatta actaagtggt atgtaaatta attagtggtg
    19441 ttataagact cattttttga ttttagtttt atattaacta agtggtatgt aaatttgact
    19501 tactatgtat tacttctaag ttacttatat cgttttatat taactaagtg gtatgtaaat
    19561 caagatattt ttaataaaat ttctgagcga actgttttgt tttatattaa ctaagtggta
    19621 tgtaaataaa aatagaacaa attatggaaa aatactagta aatgttttat attaactaag
    19681 tggtatgtaa attctcatca caacactata tacatagtaa attaacatag ttttatatta
    19741 actaagtggt atgtaaatgc atgttaaagt ggctaattgg aactatgatt acagttttat
    19801 attaactaag tggtatgtaa atagtccaga ccctaatcct cctgttgacc cagagtttta
    19861 tattaactaa gtggtatgta aatatagtca aaagcaacat cataatcacg tatattatcg
    19921 ttttatatta actaagtggt atgtaaatca acatgtcagt aatattgaaa caggaaattc
    19981 gagtgtttta tattaactaa gtggtatgta aataacactt taaaaactaa tgtattcagt
    20041 ataagagatt gttttatatt aactatgagg tatgtaaatc ttgttaatgc aaataaacaa
    20101 catagcacta ttaatagttt tatattaact atgtggtatg taaatttgca acaagtatag
    20161 gtaaaatacc ccaataattt atacagcatt ttctccttta aaatataatt attttttagc
    20221 atttgtagta aataattacc agataatatt gactttagtt ttaatgatta aaatataaaa
    20281 gtagaataat tataa
//
LOCUS       DAMJYC010000069        20132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJYC010000069 DAMJYC010000000
VERSION     DAMJYC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.070"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(306..767)
                     /locus_tag="PC472_002695"
     CDS             complement(306..767)
                     /locus_tag="PC472_002695"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2728011.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(872..2065)
                     /locus_tag="PC472_002696"
     CDS             complement(872..2065)
                     /locus_tag="PC472_002696"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2728012.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2088..2753)
                     /locus_tag="PC472_002697"
     CDS             complement(2088..2753)
                     /locus_tag="PC472_002697"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2728013.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="PC472_002698"
     CDS             complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="PC472_002698"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2728014.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4031..4152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4341..6824
                     /gene="recQ"
                     /locus_tag="PC472_002699"
     CDS             4341..6824
                     /gene="recQ"
                     /locus_tag="PC472_002699"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2728015.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6913..7024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7166..8467
                     /gene="thiC"
                     /locus_tag="PC472_002700"
     CDS             7166..8467
                     /gene="thiC"
                     /locus_tag="PC472_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2728016.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8521..8715
                     /gene="thiS"
                     /locus_tag="PC472_002701"
     CDS             8521..8715
                     /gene="thiS"
                     /locus_tag="PC472_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2728017.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8708..9511
                     /gene="thiF"
                     /locus_tag="PC472_002702"
     CDS             8708..9511
                     /gene="thiF"
                     /locus_tag="PC472_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2728018.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLFENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGKGLMAPRVAICANHMANLATQILIDM"
     gene            9574..10344
                     /locus_tag="PC472_002703"
     CDS             9574..10344
                     /locus_tag="PC472_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2728019.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10361..11464
                     /gene="thiH"
                     /locus_tag="PC472_002704"
     CDS             10361..11464
                     /gene="thiH"
                     /locus_tag="PC472_002704"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2728020.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11465..12154
                     /locus_tag="PC472_002705"
     CDS             11465..12154
                     /locus_tag="PC472_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2728021.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12342..13736
                     /gene="yfcC"
                     /locus_tag="PC472_002706"
     CDS             12342..13736
                     /gene="yfcC"
                     /locus_tag="PC472_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2728022.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14253..15620
                     /locus_tag="PC472_002707"
     CDS             14253..15620
                     /locus_tag="PC472_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2728023.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            15645..16253
                     /locus_tag="PC472_002708"
     CDS             15645..16253
                     /locus_tag="PC472_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2728024.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            16301..16672
                     /locus_tag="PC472_002709"
     CDS             16301..16672
                     /locus_tag="PC472_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2728025.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16851..17189
                     /locus_tag="PC472_002710"
     CDS             16851..17189
                     /locus_tag="PC472_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2728026.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            17655..18158
                     /locus_tag="PC472_002711"
     CDS             17655..18158
                     /locus_tag="PC472_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2728027.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            18205..19164
                     /gene="moaA"
                     /locus_tag="PC472_002712"
     CDS             18205..19164
                     /gene="moaA"
                     /locus_tag="PC472_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2728028.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLLENKCSDTNRRMYEIGG"
     gene            19168..19638
                     /gene="moaC"
                     /locus_tag="PC472_002713"
     CDS             19168..19638
                     /gene="moaC"
                     /locus_tag="PC472_002713"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2728029.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            19674..20105
                     /locus_tag="PC472_002714"
     CDS             19674..20105
                     /locus_tag="PC472_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2728030.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7832 a         2197 c         3440 g         6663 t
ORIGIN      
        1 ataaaataga taattaatat actagtaata tatgctataa ataaaaacaa tagctaaaat
       61 agtaatatat atgtttttat aatattttat gtttagggtt atatttgtaa ttattttaag
      121 cttctaaaag tagatttgat ggataacgta taattttaga aggatatgca atttgtttga
      181 taagaacgta catatgatta aattaaaaat attaagtacc aaaaagaaga aagatagttt
      241 ctccctttgg tacttaatat tttgtatcct ttacactaat ttttaatatt tattaatgat
      301 attatttaat ctagagactt aaataaattt gccatttcta gcgcattcat agcacaatca
      361 taacctttat ttccagcttt tgtacctgct ctttcaatag cttgttcaat attttctgta
      421 gttacgattc caaatataac aggtaattca ctgtctaaag atactttggc tatcccttta
      481 gagacttcac tacatacata atcataatga gaagtagccc ctcttataac acagcctaaa
      541 cagattatag catcatactt accactttta gccatttttt gagcaacaag aggaatctca
      601 aaagctccag gtacccaaac tatttctata ttttctggag aaacgtcatg tcttaaaaga
      661 cagtcttcag ctcctgataa aagttttgat gttataaatt cattaaaacg agaatttatt
      721 atacctattt ttaaatcttt tccaataagt tttccttcat atatcatttt tatttcctcc
      781 aattttattt atgttataga tttatattta taaatatctt taatttaatt ataaaaaact
      841 acttagtaga aaaatatcat catataatca tttatataat atctaaaaga tgtcccattt
      901 tatctttttt agtttttaaa tatagcttat taaagtcatt aatttcttca ttaataggga
      961 ttctattttc gatttttatt ccatatttct ctaattgatt tattttatct gggttattac
     1021 ttaaaagatt tatacttttt atgtttaaat tttttaatat ttgagcagcc atatagaaat
     1081 ctctcatatc ttcttcaaat ccaagcatta aatttgcttc aacagtatcg taaccttctt
     1141 cttgaagttt ataagcctta attttattta aaagaccaat acctctacct tcttgcttca
     1201 tataaattaa aactccactt ccattttcca caattttctt catagaagat tctagctgta
     1261 atccacaatc acattttaga gagtggaaca catctcctgt taaacattca gaatgaagtc
     1321 taactagagt attttctata tttatatttc cataagtaag ggcaatgtgt tcttcatttg
     1381 aataagtatc tctatatcct atgatttcaa aatttccata ttttgtcgga aggaaagcac
     1441 tagatacttt ttctatagta gtttcattta ttttgcggta ttctataagg tcttttatag
     1501 tgatgatttt taaattatgc ttttctttga aaatcattaa atcatcaact cttgccattg
     1561 taccatcatc tttcattatt tcacagataa gaccaatctc tttaaaacct gcaagtctag
     1621 caaggtctac tgaggcttct gtatgtccat ccctgacaag aaccccacca gtttttgcta
     1681 ttaaaggaaa gatgtgacca ggcgttttga aatctttact tgtagaattt tcatcacaga
     1741 acaattttac agtttcagct ctttcatatg cagatatacc agttagagta tctgctccat
     1801 ctatagtaac agtaaaggct gtttgaaatg tatctgtatt ttctctaacc ataagaggta
     1861 tattaagact tttaaatttt ttttcagtgg caggtagaca aattagacct ctaccatatg
     1921 tagccataaa atttattgat tcaggagtag ccatttcagc tgccatcaat aaatctcctt
     1981 cattctctct actttcatca tcaactacga ttaccatttt cccatttttt atatcttcaa
     2041 tagcttcttc aatagtatta aacatgtaat tcactccttt attattttta aaaaccattt
     2101 ttaaataaaa aatctttatc tatatttgat ttatggttac tactattaga atttaattcc
     2161 ttgtagttat tagccataaa attattaaca tactttccaa ttacatcgct ttctaaatta
     2221 acgaaatctc caacattctt tgtcaagaga atagtgttaa ttttagtatg aggtataata
     2281 gaaaccttaa atatttcatc atctaaatat gatatagtta aacttacacc atcaatagca
     2341 acagaacctt tgagtatcat tgaggataaa agttttttat caggtcgtat acttactaaa
     2401 actgcatttt catctttttc aaatttgcag atagttcctt taccatcaac atgacctgtt
     2461 actatatgac caccaaatct tgaagaaaga ctcatagctc tttcaagatt taccttgtca
     2521 tttgcattta ataaacctaa atttgtggaa cgaattgtct ccatcataac atcggctgta
     2581 aattcatttt ttccgaggtt tgaaacagtt agacaaactc catttacggc tatactatct
     2641 ccaagatttg tgccatctaa tattttattt gatagtatgg ttacttttcc actcttacca
     2701 ttagcagtaa tttttctaag tattccaact tcttctacta tgccagtaaa catcctaacc
     2761 tcctatatga atgtattaac aaaaatttaa tttaaaacat ctgattctat aagaatatcg
     2821 ccatctatca aagttacatt attgattgtt aactgtgttg aatctgacaa atgatttatt
     2881 ccttctccag aaacgaatgt aggtgcatct ttaccaccta taatcttggg cgatagaaat
     2941 aatttgactt tatcaactaa cttatttcta aagagactgt cattgagagt agcaccacct
     3001 tcaactagaa cagaatcaat gtttaactct cctaatttat acattacaaa ctccaaatct
     3061 actaagttgt tttttgaagg agaaattatt gtttcaacat tattttcttt taacatagat
     3121 aattttatac tttctttgca acatgtgaaa actatagttt tactggtttt atctttaacc
     3181 aaattactat taagtggcaa ctttaggtgt gtatctatta ctattcttgt aggatgagac
     3241 actttttctt gattgagcct acaagttagt aaaggattat cgttcaaaac tgtatttatg
     3301 ccaaccatta tactttggta tttatttctg tatttatgag ttagatacct tgatgattca
     3361 ttagatatcc attttgattc tagtgatttt gtggcaattt taccatctag acttactgct
     3421 gattttacaa tacaaagagg tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa
     3481 tctcttgctt catcttctaa tattcccaca gtaacatcaa tattgttatc ttttaatttt
     3541 tttactccat taccacaaac aagtggattt gggtctaatg tagatattac aactctctta
     3601 atcttatttt gtataatttt atctacacaa ggaggcgttt tcccataatg agaacatggt
     3661 tctaagttta cataaagagt agagtctttt agactttgtt tagcactgtt tatagcattt
     3721 acttcagcat gattagagcc aaatttctca tgatatcctt tgcctattat attactatct
     3781 ttaacaatta cacatccaac taatggattt ggatttacaa agccttctcc atttttagcc
     3841 aattcaatag cttttttcat ataataaata tctttttctt tttgattcac aaaattcaca
     3901 atatgtacac ctcctgttta attggataat ttttaaacaa aaaaccctga gaattaactc
     3961 agggtttaga ataatctaca ataaatgttt aataaatgta gaacaaaatt gctctacaaa
     4021 aacactcatt aaatatcttc ttccatccag actttactgt cggctttgga atttaaccaa
     4081 atcaaccaag ttttacattg gctcgcgggc tataccgccg attgggaatt tcaccctacc
     4141 ccgaagatta agtattaaat ttatatttag aatgtatcat aaattatttt actttgcaaa
     4201 ctattttttt aaaaaatata tgttaatata taatatagaa tgtctataaa ctttgaaata
     4261 ccacgattgt gaatataaaa ataaaaattt gaacataatc aggaaagaag tctaattaat
     4321 ttaaattagg agagtaatat atgaataaaa aacttttaga aatactatca aaatactatg
     4381 gatatacaag ttttagaaaa ggtcaggaat ctataataaa ttctatctta agcgaaaaag
     4441 atgtgctagc tattatgcca acaggagggg gaaaatcaat atgttatcaa attccagctt
     4501 tgattttaga tggaatgaca atagtaattt ctccacttat atcacttatg aaagaccaag
     4561 tagatgcctt aaagactatg ggaattagtg ctgcttttat aaatagttca ttaagcagca
     4621 aagaatttaa tgaaatatta aataatatta gaaaaaataa ttataaaata ctctatattg
     4681 ctccagagag attagatgca caagagttta ttgagctaat aaataataac aatgtaagtc
     4741 aagttgcgat agatgaagca cattgtgtat ctcaatgggg gcatgatttt aggttgagtt
     4801 atagaaggat ttctgatttt ataaaaaatt tgcctaaaag acctatagtt acagcattta
     4861 ctgcaacagc ttcagaagag gtaagaactg atataataaa tttactgtgt ttagaaaatc
     4921 cagattatta tataactgga tttgatagag agaatttatc aataaatata gtaaaatcat
     4981 ctagtaaaaa taagtatatt ttagattata ttcaaaatca taaaaatgag tctggaatta
     5041 tatatgttgc aactagaaag gaagttgaaa atatatataa tggtttatct aagagaaata
     5101 tatctgtgtc aaaatatcat gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag
     5161 attttataaa tgataatgtg gatataatgg tagcaacaaa tgcatttgga atgggtattg
     5221 ataaacctaa tataagatgg gttatacatt acaatatgcc tcaaagtata gaaaactact
     5281 atcaagaaat aggtagagct ggtagggatg gagaaaaaag tgagtgtgta cttttatttt
     5341 ctccaggaga tgtacatata caaaaatatt taattgatat tggagttgaa aatcctgaaa
     5401 gaaaattatt tcagcataag aagcttcaat acatgattga cttagtttac agtaattcat
     5461 gctatagaaa gacaatattg agttattttg gagaaaatta tatagacaat tgcaataatt
     5521 gtagcaattg tttagtagag ggtgaaatag tagataaaac tatagatgca caaaaagtca
     5581 tttcatgtgt agctagaatg aaaagaagtt atggtgttac tactattgta gatgtgctta
     5641 gaggttctaa gaataaaaag attttacagc ttggattaaa tactttgtct acatataaca
     5701 ttatgagaga ttattctagt gaagacttaa agaattttat aaatacattg gtttctcatg
     5761 gttttttaga tttagttgag actctaggca atggaaatag aggtagtttt cctactataa
     5821 gattaaatga gatgtctatg aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa
     5881 ttgttatgac aaaatcattg gaagtagagg atgagttata taatgcttta agagaactta
     5941 gacattcaat tgcaagtgaa gaaaggatag ctccatatat ggtatttgga gatggtacac
     6001 ttagagccat gtctagtagt tatccaatta ataaagaaga aatgctagat atctctggag
     6061 taggagaaat taaataccaa aaatatggta gagattttga agcagtaatt aaagaatatg
     6121 tcgaaaaaaa taatataaat aaaaaaatag atgaaaaaaa tgtcgattca aagaacgtta
     6181 atagtgattt cttagaggtt aatacagata aagttttata tgaaaaactg ttagtcatta
     6241 gagaagaata tgcaacaaag gaaaaattac ctgcatatat gattttagcc aataaggctt
     6301 tgaaagaaat aagtggaaga tatccacttg atgaagaaca attaaaagat atttctggca
     6361 taggtggagt aaaatttgaa aaatatggag ctagtatttt ggcagaggtt aataaatatg
     6421 taaaagaaaa taatattaat gttatttggg agaaaaaggg caaaaggaag ttaatattag
     6481 atggagaaag tagaaaaaat aatgaaatag cattagattt gctaaatcaa ggaaaagata
     6541 tacaaagtat cagtcaagaa atagaagttt cactgtcaac cattattgga tatgtgcatg
     6601 actatgtaaa ggatggacat agtatatcat ttgaattagg actagaaaat ttttataatg
     6661 aagataaaaa gaagatgatt ttagaagttt gtgaaaaagt tggctatgat aatttaaata
     6721 acataaaaag aaaacttcca gattcaataa agtatgaaaa tataagagct gtgatacttg
     6781 actcttatct tgaaaactta aaaaataata ttatagcaga gtaattttta acttaataaa
     6841 aaataaaatt tgtattattg tgttgaacta ataattttgc tgtgttatac tgaatttgta
     6901 aaaataatga aaaattagag ctaggggcgc tagaattggc tagctgagag ataaagccgt
     6961 aacttacaaa ccctaataac ctgatcaaga taatgcttgc gaagggaagc tttttaatat
     7021 aattttatta tataaatagg caaattattc aatgttagga gaaatttgtc taagtgtata
     7081 tacgattata agagtgcttt ccaaacgagg aaaagcactt tttttatttt caaaaactat
     7141 aactttaatc ttggaggatt gtaatatgaa ctatacaaca caaatggatg ctgctagaaa
     7201 aggcataata actaaagaaa tggaaatcgt ttctcaaaaa gaacaggtag atgtaaatga
     7261 attaagagaa ttaatagcaa atggacaagt tgttatacca gctaataaaa atcataaatc
     7321 tttaagtgca gaaggtgtag gtaaaaattt aagaacaaaa ataaatgtaa atctaggaat
     7381 ttcaagagat tgtaaggata tagaaaaaga acttgaaaaa gttagagtag caatagacat
     7441 gaaagctgaa gctataatgg atttaagtaa ttatggcaaa actagagaat ttagagaaaa
     7501 ggttgttgaa atgtcacctg caatgatagg ttcagtacct atgtatgatg cagtaggata
     7561 tttggaaaaa gaattaaaag atataacaga agaagagttt ttaaatgtaa taagacaaca
     7621 tgctattgat ggagtagact ttattactat acatgcaggt cttacaagaa gtgtttgcca
     7681 aaaaataaaa aatcatgaga gattgactca tatagtatct cgtggaggtt ctttattgtt
     7741 tgcatggatg gaattaaata ataaggaaaa tcctatttat actaattttg ataaaatatt
     7801 ggatatatgt gaagaatatg atgttacatt gagtttagga gatgcttgta gaccaggttg
     7861 tataaaggat tctactgatg gtgttcaaat tcaagaactt gtagtattag gtgaacttac
     7921 taaaagagca tgggaaagaa atgtacaagt aatgatagaa ggtcctggac acatggctat
     7981 tgatgaaata gaagcaaatg tagtattaga aaagagatta tgccatggag caccttttta
     8041 tgttcttgga ccattggtta cagatatagc accaggttat gaccatataa caagtgcaat
     8101 aggaggagct ttagcatgtg ccaaaggagt agatttttta tgttatgtaa ctccagcaga
     8161 acatttaaga ttgcctaatt tagatgatat gaaagagggt ataatagctg caaagatagc
     8221 tgctcatgca ggagatatag ctaaaaatgt taaaggtgct cgtgaatggg ataataagat
     8281 gagtaaagct agagctgatt tagactggtg tgagatgttt agacttgcaa tagaccctga
     8341 aaaagctaag agatacagag atgaatcaac tcctactcat gaggatagtt gtactatgtg
     8401 tggaaaaatg tgctcaatga gaacagttaa gaaaatatta aacaatgagg aacttaatct
     8461 aatataattt ttggctagta tgactggaat aaaaagaagc tgaaaagagg aggtgattta
     8521 gtgaaggtta atgggaagga aattgaattt gaaaaagatt tgactgttat agatttatta
     8581 aataaatata atttaaaaag tgatagagtt gtagttgaag tgaatttaga gataattgaa
     8641 gaatctaact acaatacata tgtattaaaa gacgaggaca ttgtagagct tattagcttt
     8701 attggaggtg gttaaaataa aaatatttgt aaatgaatta ttaacagatg ttgaggaaaa
     8761 tacaactgct tataaagtta gagatagcta taacaataaa tgtgatgtat tggtgttaaa
     8821 tggatatcca attaaaacag atatgcccct ttttgaaaat gataaattaa cacttataca
     8881 aaaaggagtt aagccatcac ttgatgaatt agaaagcttg ttgattgcta gacatactcc
     8941 aaatatacat aataaattaa aaaaagggaa agttgctata ttgggattgg ggggacttgg
     9001 ttccaatata gctatctctt tagctagaat aggagtggga gaacttttac ttatagatta
     9061 tgatgttgta gaaccatcta atttaaatag acagcagtat tttatagatg atataggaaa
     9121 actaaaaact gatgccatga tagaaaatat aaaaaaaata aatccattta taaaattaaa
     9181 taaacgagat atttttttaa acaagaacaa tatggatact attaaggatt cagatttaat
     9241 aattgaggcc tttgatgatg cttcatgtaa agcacaggta tgtaattatg tattaataaa
     9301 tttaaaagat aaatatttaa tagcatcatc tggtatggca ggttactatg attcaaatat
     9361 aataactact aagaagataa aagacaggtt ttatatatgt ggggattttg tgaatgaagc
     9421 taaatttggt aaagggctta tggctccaag ggttgccatc tgcgcaaatc atatggcaaa
     9481 tttggcaacg caaatactta tagatatgta aatattaaaa ttagatataa aaaagattga
     9541 atattaaaaa aatttaacgt ggagggaaat aatatggata aattgatact tggtggacat
     9601 gaatttaata gcagattatt ggttggaaca ggtaaatatg gttcaaataa tatattgcct
     9661 gaggtaataa aagaaagtgg aagtgaaata ataacaatgg cacttagaag agtggattta
     9721 gacaataaac aagaaaacat attaacgtat attccaaaag agatgacaat acttccaaat
     9781 acatcaggag ctactaatgc agaagaagct gtaagaatag ctagaatatc tagaaaaatg
     9841 ggatgtggag attttataaa aatagaagta atatcagata ctagatattt acttcctgat
     9901 aatgaagaaa ctataaaagc tacaaagata cttgctgatg aggggtttat agttcttcca
     9961 tatatgacac cagaccttta tgctggaaga agacttatag aagctaatgc agcggcagtt
    10021 atgccacttg gagctccaat tggttcaaat agaggtcttc aaatgaaaga aatgataaga
    10081 ataatgatag atgagttgga tataccaata atagtagatg caggtattgg aaaaccatct
    10141 caagctatgg aggctatgga aatgggagct gatgcagtac ttgtcaacac tgctatagct
    10201 tcagcaggag accctgtaca aatggctaga gcatttaaat tagctgtaga aggtggaaga
    10261 gaggcatata ttgcaaaaac aggaaatgtg tcagaatttg caaatgcatc ttctccttta
    10321 acaggttttt taggcaattt atagaaagtg gtgattggtg atgagttttt atgatgtaat
    10381 agaaaaatat agggattttg attttgatgg atatttaaat aatgttactg ataatgatgt
    10441 tttaagaagt ttatcaaaag acaagcttga agattttgat atattaaatt tgctttctaa
    10501 aactgcagta aaacatttag aagatatggc acaaaaagca cataaattaa gtgttcaata
    10561 ctttggtaaa acagtatgtt tatatacacc aatgtatata gcaaattatt gtgttaatca
    10621 atgtgtttat tgtagctata atataaagag tggaataaaa agaaaaaaat taactatgga
    10681 tgaaattaga gaagagggag aagcaatttc aaaagaaggt ttcaaacatc tacttgtttt
    10741 gactggagaa agtagttttc actcaagtgt agaatatata ggtgaggcta tagaaatatt
    10801 aagagagaaa tttccctcta taggtataga agtttatcca atggaagtag aagaatataa
    10861 gtacattgta gataaaggtg ttgaaggatt aacagtatat caagaaactt atgacgagga
    10921 aatatataag agagtacata taaaaggccc aaaatcaaat tataaattta gactagatgc
    10981 tcctgaaaga ggggcaaaag caggtatgag gactttatca ataggtgcac ttttagggct
    11041 aaatgatttt agaaaagaaa ctttttttac aatattgcat gggaaatact taaaaacgaa
    11101 atatcctcat attgagcttt catattcaac accaagaatg agacctttta aagggtgttt
    11161 tgaggaattg gtagatataa gtgatactga tttagttcaa gctatggtat gtatgagatt
    11221 atttgaccct catgcagcta taaacatatc cactagagaa aatttagaaa tgagaagtca
    11281 tataatacct ttaggagtaa ctaaattgag tgcaggtgta tctactgatg taggaggtca
    11341 ttctcaagat gaacatgata ctgctcaatt taagataaat gatgaaagta ctgttaaaga
    11401 tgtggagaaa atgctaaact caattggtta tcagcatgtg tttaaagatt gggaaagatt
    11461 ttaaatgtat ctgataacaa acagaaaatt atgtagtgag gaacgatatt tagaagttat
    11521 taaagagtct atattgagtg gtgtagaaaa tataataata agagaaaagg atttggaata
    11581 tcaagagcta agaaaattat atatgaaaat aaaaactaaa atcaattgta tagattttca
    11641 agagcaaata tcagatgaaa gtttgaaaac taatataaat caaaaagagt gtagaaataa
    11701 attcaaggtt aattttataa taaatagtaa cattgaattt tttgaaaaag tggattgtca
    11761 aggtatccat cttcctttta aattgttctt gaatttaatt gaaaataagt ataattttaa
    11821 tgaaaataaa atactaggat tatctttaca taaagtagag gaagtagatt atttagaaaa
    11881 attaataaga aatcaaaata taaaaataga ttacataaca ttatcacata tatatgaaac
    11941 taaatgtaaa gaaggattaa atccaaaagg aattgagctt ttaaaagaag ctaaaaagat
    12001 tactgatatt aaaataattg cacttggggg aattttacca tctaatgtca aggaaacatt
    12061 aaaatactgt gatgattttg ctataatgtc aactataatg aggtctaaag atattaaaaa
    12121 aaccatatca aattataatg aaaagttgaa ttagtaaata tagtatattg aatttttaac
    12181 ttaaataaaa ataaaaaagt tatcttttaa attttaaaag gtagcttttt tattttatat
    12241 ctttgctaat aaaaaattgt aaaaagggaa aactttagtc agagagatta aacaatatcc
    12301 tagaaagatt taaaatttag taaagtatgg aggacattat aatgactgac agtaaaaaga
    12361 aacgatttat tataccacac acttttacaa taatattttt actaatagtc ttaatggcaa
    12421 tattgacttg gatagtacca agtggtgaat ttagtagaga agatgtagaa ggtagaatgg
    12481 tagttgtacc agggacttat caaagtgtag aaagtaatcc tcaaggattt gcagatgttt
    12541 ttaaagcacc aatagaaggc tttatcgatt cagctgaagt tgtaggtttt gttttaatag
    12601 ttggaggtgc ttttggtatt gtaaatagga ctggagctat agaagcagga atagcttcgg
    12661 ttatctctaa gtttaaggga aaagaacttc ttataattcc aatatcaatg attttatttg
    12721 gacttggagg tacaacattt gggatggcag aagagacatt accattttat atgatatttg
    12781 ttccactcat gacatccttg ggatatgatt ctctaacagc aatagcaatt gtatttatag
    12841 gagcagcagc aggagcagcc gcttcaacaa taaatccatt ttcagttggt attgcacagg
    12901 cactagctca acttgctcct ggttcaggag taacttacag agtagttatt tttatagcat
    12961 atatactaat aagtatagca tttgttatgt actatgctaa gaaggtaaag gaaaatcctg
    13021 aaagttcaat agtatatgaa atagataaaa ataataaatc aatacttaat caaacttcaa
    13081 cagaaataaa agaatttact tttaaagaag tgtctgttct tgctatattt ggaataggga
    13141 tggcaataat gatttggggt gttttaagtc aaggatggta cattccagaa atatcaatgt
    13201 gttttgctgc aataggtgtg ctttctggat tggttggtag attatctcaa attgaaatct
    13261 cagaaagttt tatagatgga gcaaaagatt tagcatctgc agctcttgct atagctttgg
    13321 ctcgtggaat cgtaataatt gctcaaaatg gatttataat agatactata ttaaattccg
    13381 cagcagcctt tctaggtggg cttccaaagg ttatatttgt atatttatca tttttcttgg
    13441 aagctgcatt ggcattttta attccatcat cttcaggtct tgcttcactt acagtacctg
    13501 tacttgctcc tcttggtgat ttggttaata tatcggctca agtgatagta accgcttatc
    13561 aatttggtgt gggtcttaca aactttataa ctccaacttc aggagtactg atgggagcac
    13621 ttgcagtagc ccatattcca ttttcaaaat gggtaagatt tataatgcct atcttgttga
    13681 ttttgacagc tgtatctcta gtctttttaa caataggtat ttatataggg ttttaattaa
    13741 gtgtagtttt atttaagata tttagtaatg gtataaaatg ataatatgtt ttcaataaat
    13801 tgccactaat ttaattagtg gtaatttgtt ttttgtattt aaaaataaat gtatttactt
    13861 gtattttgaa ttaatatcta cttagacatt gttttaattg ttatattttt atttaattaa
    13921 gcattgattt atcatttttt atatttcata taacaatgca aaaaataatt tttatatagt
    13981 aaatgacaaa agtttacaat aaatgtatat tttcttactc attccttaaa attactcatt
    14041 tagtttaata actctcattt catatgtaaa gttggtaaaa aaattaaaat taaaagtaaa
    14101 aaactaaata aattaatgtt aatttgaaat atatatgaag gtttttggag aatacttact
    14161 tatgatagga aaaaatgtta aaataatagt gtaatcaaat ataaaggact gaaataatta
    14221 acagttatta aatattagga gtgtaaaaat ttatgggatt aaaagaattt gattttattg
    14281 aagagtctat tgatatgttg aggacaatgt caccaacatt agaaactata tcagatgaaa
    14341 tagaagagta ttttgaaaat atcttggatg aaaagaatca agaatatata aatgtaacct
    14401 ctagaataaa atcagaatca agtttaagag aaaagataat tagaaataga tatttaaaaa
    14461 aatatggaga agcaagtaat cttattcata atgtttctga cttaataggc ctaagaattg
    14521 agtgtagatt tattgaggat gaaaacaaaa tatatagact tttgagaaga tattttaata
    14581 aaacagatga caaaataaac tattataata aagagaataa gaatattaaa ttaaaactat
    14641 ctgagagaca acccaataag caaaaaaatg gatttgaaat atataaaata gatggagttt
    14701 ttgcgtattt agatagagaa gtaaaatttg aacttcaaat taaatctctt gtaaatgtgt
    14761 tttggagtga aatagaacat aaaataattt ataaaaacaa tacatattta cttgctgata
    14821 aatttataaa ggatatgatg gactctataa agaataatct tactatgata gataatcagc
    14881 ttttaagtat ttataaaaac tttcattcag ggaagtcttt taatatgaaa gtaagtaaaa
    14941 aagaaattga aaaattattt gcgaaattag tctatgacgc tttttctgaa aagatgaata
    15001 aaagtatagg ttttgttgta gattttaaaa aaccatgtga gactatattg aactattctt
    15061 ttaataagca agatataagt gatgaagttt taggtaattt tatgcttgat gagtttgcaa
    15121 gattaaatga aattgtaaac aaggatatag attttaatga gcaaatagaa tttgaaagag
    15181 aaccaacatt tgatgataag ttttgtaaag acctaggaaa tcattttaga agtagattaa
    15241 acacagaatt tccatggaac ctatttttta gaatattatt tgaaatagag ccatataaca
    15301 ataccaaaga ctttgaaaac tttgtagagt ttataaaaga aaatgtgcta ctagaagaaa
    15361 atagggagca attattatgc caatttgaag aagatagcaa gcttataatt gaagatatat
    15421 atgattgtat atttaatagt ataagtgaga ttgatagtgt tgaaatttta tataactata
    15481 atcttgaaaa aataaacttt acatcaagtg aaatcataaa ttgtatatgt agagaatatg
    15541 agtgttatgg tgaatactta gaagaaaaag aaaagcttat gaatacattt aaagaaaaga
    15601 ttattcaaat ctttgagtaa taggtcaatg ctgaagtggc gctaatgcgc catttttcaa
    15661 atatatatga attaagtaaa attattttgg aggaggcgat ttgtatagag aaaacaggtg
    15721 taattttggc agggggaaga aattctagaa tgggtagaga taaagccttt ttagagttac
    15781 atgataagct ttttattgaa attgcaatag aagcatttaa aaactttgat gagttgataa
    15841 taatatctaa taatgaagaa ttatattcta aatacgatat taaagtttat aatgatatag
    15901 taaaagatgt tggtccaatt ggaggaatat atactgctct tgaatatgca aagtatgata
    15961 tagttacaat tgcatgtgat atgccatatc taaataatca aatagtcgaa agaatagcta
    16021 ataaaatgga tgataaaagt gtgatttcag tgacaaatgg aaagttacaa ccattgtgtt
    16081 ctggatataa aaagagtata ataaataaag tatctttatg tataagggaa aatgatttaa
    16141 agctcagaag ctttatagat aaaatagata aaagttatat atactttgat gaagaagatt
    16201 tatttttaaa tgtaaacact gttgatgagt ataaaaaatt aactaaagtt taattagcta
    16261 tggttgtaaa atagagtagg atttatatgg aggagagttt atgaatttac ttaaatgcga
    16321 ttcttgggca gtaatattaa ataatagcga taaagaaagt aaagcatata aaatattaga
    16381 tgaacttaaa aggaatatgt acaaagttgt tgctatagat gaagagaaaa aacctattga
    16441 aggaatagat gtgtatgaat gtttaaaaga tgttccacac aatattgatg ttgtagctat
    16501 tattgataaa caatctaaga tggatgtgat tttagaagaa gtagagcttt tggatataca
    16561 aaatatgtgg tttgaaaaag gaagctttag tgaaatgatg attaaaaaga ctaaagattt
    16621 aaaattaaat atagaatata atttaagttt atatgatgag ttgactagat aaaattatat
    16681 ttcttaataa taaaaatata attagttaat aaaacatatt attttatagt atagattggt
    16741 taataaaagt acagattggt taatgaaaca ttaaattaca atatttgtta aaaaaattgt
    16801 gagttacatg atataatgta ttaaatagga tactaaggag ttgatatatt atgttaatag
    16861 taactacaga aaaagtagaa ggtaaaaaga tatcaaaggt tttaggatta gtgagaggaa
    16921 gtacaataag agcaaaacat gttggaaaag atataggagc aagttttaag aatcttgttg
    16981 gaggagaact tactggatat aatgaaatgc tcactgaagc aagacaaatt gccataggta
    17041 gaatggttga agatgcagaa gctaaaggtg caaacgcagt aatagcattt agactgtctt
    17101 cagcttcagt tatgcaaggg gcagcagaaa tgcttgctta tggaacagca gttgttttag
    17161 aagatgataa tagtattctt gaaaaataaa atataaatag ttaaaaagag gcatctttta
    17221 aaaattttat ggatgccttt ttttatttga aaaaactaat ttgagaagaa attttaacta
    17281 ttatgacaat aaaattaggg tgatattata taaataagta aaattatatt atataatgta
    17341 tggtatagac tttaaaaatt ttatttgtag agaaaaatat ggacaatgtt ataataatta
    17401 tagaaattaa aagtacttac attcattatg tatgtgacat gtataattag ttttatattt
    17461 aatacagttt tgaagtaata tcttcatttt gagaacgttg gaataataaa aaatgtatta
    17521 ttataggtga tataaataaa aaataaatat aatatgttat attgctgata gaaaagagaa
    17581 taaaagaaaa taggttttat taaaagagat ttttatttat aggacttaat caatgtatag
    17641 aggggtgaga acatatgttt aatgttgcaa ttataacact tagtgataaa ggatatgaag
    17701 gaaaaagaga agatataaca ggaaaaaagt taactgaatt tgttgaaaat acaggagctt
    17761 ataaagttac tgaatatgta cttataaaag atgataagga aatgctaaag gaaaatatta
    17821 taagactttg taatagtaat aaaatagatt taattcttac aaatggaggt actggttttt
    17881 caaaaagaga tataacccca gaagcaacta aggaagtgtt ggaaaaagaa ataccaggtc
    17941 tgagcgaata tatgagaatg aaatccacag aaattactaa aaaggccata ctaagtaggg
    18001 gtgtaagtgg aataagaaat aattctataa taataaattt acctggaagt cctaaaggtg
    18061 cagttgaaaa tctaagtttt ataattgatg ttttagacca tggcatagaa gttttaagag
    18121 gagaagctac agaatgtgcc actaaaaaaa gtgaatagta tataatctag atagataaaa
    18181 aatagacaaa gaggtagata aagtatgatt gataagtatg gaagaaaaat agactacctt
    18241 cgtatttcac ttacagataa gtgtaattta aggtgtgttt attgtatgcc accagatgta
    18301 aaatttgata aaaattatat aaatgaaaat ctaagctttg aagactacaa gtttattata
    18361 aaggctatgg ctgaacaagg tataacaaaa attagattta ctggaggaga acctctttta
    18421 tatcctaaat taaatgagct tataaaattt acaaaagaag aatgtggaat aaataacata
    18481 ggaatgacca ctaatgctat tgggctttct gatagaatct ttgagcttga aaaatgtggc
    18541 ttaaataaag ttaatataag tttagattct ttgaaggaat ataagtataa atccgtaaca
    18601 agaggaggaa acttaaaaga tgtcttaaaa tcgattgaat cttgtttaaa cttaggtata
    18661 aaagtaaagt taaattgcgt tgctataagt gggtttaatg atgatgagct taaagatttt
    18721 atgtttttaa cagttaaatc accaattgat gtaagattta tagagcttat gccattaggt
    18781 gaggctaata gagtttataa gaagggttat tttaatgtaa aagaaatgat tgaaaaaata
    18841 tatggtttat ataaagtcaa aaatgatgaa aaaagtacag cagaatatta tatgtttaaa
    18901 ggagctaaag gaagaatagg aattataact ccattaagct gttcattttg taacacttgt
    18961 aatcgtatta gacttacttc tagtggtgca ataaaattat gtctacattc aaaagaagaa
    19021 atagatataa aaccattttt acataaacca ttgttattta gagaatctat gaaagatata
    19081 ataaaacaaa aaccagaaaa acatcattta ctagaaaata aatgtagtga tacaaatagg
    19141 cgtatgtatg aaattggagg ataagtaatg ttaacacata taaatgaaaa agggtatgcc
    19201 aagatggtgg acataagtga aaaagatgat actaaaagaa cggccgtagc tacagctaca
    19261 attactctta gtaaggaagc attagaaaaa gtaaaaaatg gtcagataaa aaaaggagac
    19321 gtattgtctg tagctcaagt tgcaggtata atgggagcta aaaatacatc ttcaaacata
    19381 ccaatgtgcc atcagattaa attagttgga tgtgatttag aatttgatgt agatgattct
    19441 atgtctgcta ttaagatata cgcaacctgt aaatctcttg ggaaaactgg agtagaaatg
    19501 gaagctttaa atgcatgttc tttagcagca cttacaattt atgatatgtg taaagcagtt
    19561 gataaaaaaa tggttataag taatatccat cttgtggaga agactggtgg aaaatcagga
    19621 gattttaagt tcgagtaaat taataatata ttgggttaaa atttggagga aatatggcga
    19681 aggtagtgtc aataaatata agtgagaaaa agggtgtaat taaagttcct gtacaatgtg
    19741 cagagctaaa aataaatcat gggatagttg gagatgcaca tgcaggaaac tggcatcgtc
    19801 agataagctt acttggaaaa gaaagcatag ataaaatgaa aatgaaaggc tttgaccaat
    19861 taaaatatgg agattttgct gaaaatgtaa ctacagaagg gatagaagtt ttttccctgc
    19921 ctgttggtac aaagttaaga atagggcaat gtgaagttga agtaactcaa atagggaaaa
    19981 aatgtcacaa tggatgcgaa attaagaaat tgacaggtga ctgtgtgatg ccacgagaag
    20041 gaatttttgt aaaggttcta aaagaaggaa ttataaatgt gaatgattca atagatgtaa
    20101 tctaatgaag tcactattaa aatataatta ta
//
LOCUS       DAMJYC010000070        20102 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJYC010000070 DAMJYC010000000
VERSION     DAMJYC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.071"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(34..2145)
                     /locus_tag="PC472_002715"
     CDS             complement(34..2145)
                     /locus_tag="PC472_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728031.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(2135..2887)
                     /locus_tag="PC472_002716"
     CDS             complement(2135..2887)
                     /locus_tag="PC472_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728032.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(3978..4154)
                     /locus_tag="PC472_002717"
     CDS             complement(3978..4154)
                     /locus_tag="PC472_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728033.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4258..4866)
                     /locus_tag="PC472_002718"
     CDS             complement(4258..4866)
                     /locus_tag="PC472_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728034.1"
                     /translation="MGKFLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(4965..5789)
                     /locus_tag="PC472_002719"
     CDS             complement(4965..5789)
                     /locus_tag="PC472_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728035.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(5967..6860)
                     /locus_tag="PC472_002720"
     CDS             complement(5967..6860)
                     /locus_tag="PC472_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2728036.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7117..7554)
                     /locus_tag="PC472_002721"
     CDS             complement(7117..7554)
                     /locus_tag="PC472_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2728037.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(7811..7960)
                     /locus_tag="PC472_002722"
     CDS             complement(7811..7960)
                     /locus_tag="PC472_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2728038.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(7973..8203)
                     /locus_tag="PC472_002723"
     CDS             complement(7973..8203)
                     /locus_tag="PC472_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2728039.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9020..9241)
                     /locus_tag="PC472_002724"
     CDS             complement(9020..9241)
                     /locus_tag="PC472_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2728040.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            9821..10078
                     /locus_tag="PC472_002725"
     CDS             9821..10078
                     /locus_tag="PC472_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2728041.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            10547..11785
                     /locus_tag="PC472_002726"
     CDS             10547..11785
                     /locus_tag="PC472_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2728042.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
     gene            12709..14010
                     /locus_tag="PC472_002727"
     CDS             12709..14010
                     /locus_tag="PC472_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2728043.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            14086..14361
                     /locus_tag="PC472_002728"
     CDS             14086..14361
                     /locus_tag="PC472_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2728044.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            15696..15926
                     /locus_tag="PC472_002729"
     CDS             15696..15926
                     /locus_tag="PC472_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2728045.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            15919..16500
                     /locus_tag="PC472_002730"
     CDS             15919..16500
                     /locus_tag="PC472_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2728046.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(17079..17756)
                     /locus_tag="PC472_002731"
     CDS             complement(17079..17756)
                     /locus_tag="PC472_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2728047.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(17945..19309)
                     /locus_tag="PC472_002732"
     CDS             complement(17945..19309)
                     /locus_tag="PC472_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728048.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            19437..19958
                     /locus_tag="PC472_002733"
     CDS             19437..19958
                     /locus_tag="PC472_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2728049.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         7622 a         2341 c         2998 g         7141 t
ORIGIN      
        1 tttattactt taaaaatcta ataacctttt ttattataaa ataccaataa tttttttgaa
       61 tttcttataa gttaatagat atataaggta catataaacc ccacaaatcc ctataaaacc
      121 taagtttatc aaaagagcat tgacctcact attgacgatt ctaccatcat tatttaacat
      181 aatcataaaa ttaaacagat aaattaaaaa acttcctgca ataagagggt atgaaaatac
      241 tttctttaat tgatttctaa tacagaactt tatataatca ttgtttgctc ctaatcgatt
      301 taaatccata aagacctgct tgtgatttat agctatagta acaccccttg tatatgcaat
      361 gattcctact gctgtaaaac atatgattgc cacatatatg aataacatta agaagactgc
      421 catgtttttt atcatatctt ggctttgtaa aattttaaag ttaggatagt atttccaatt
      481 aagatacaac tgattgttgt ttgggtctag ttttaaatga ccttcaccac ttggatattc
      541 ttcatccata aaatattttc ttccagttga ttcataacgt ttttttacgt attcatcata
      601 accactgaac atgcccattt ccttagatgt tctttttata atttctttct ttaagtcatt
      661 ggcaaaagca taggtatctt tatctacatt aaatagtata gaattattgt aattttttat
      721 ggataaatct ttagtaaatg atttataatc ctcctcatta aggacatagg cgttcatact
      781 tctattttga aaaaaagaac cattaaatat tatttttcca gcatatctaa aggggatttc
      841 tttttctgtg actgggcttg atataaattt ggtatattta taatcttgat tttcatttcc
      901 tttatttaca acagcaagat attcaccatt ttttatagtt attggtttgc caattagctt
      961 ggaaaaatct ttggaattaa agaacatatc ataggagctt tttttcatat ataccttttc
     1021 gatagttcca tctttatgat atattgattc tacaccatca acaattaatt ctatagaagg
     1081 aatttccata tagttttgaa tttttatatt gtgtttatct gctaattgtt caatatcttt
     1141 ttttgatacc tgtttatctc tttgattgta ggtaaaagta taatctatag ggttattttt
     1201 aagagtatca tagatacctg taaccatatt aggaacatag aatacagcaa ataatgctcc
     1261 tgcaatcaaa aatgaaataa cacacatgtt ttttactgtt tgtcttccca taaatctcat
     1321 catgctagtt gatatgatgt ttttatagta tttctctgga tgttttcctc ttttagcatg
     1381 tacaactatg tatgtcataa tcatatatat tccaataaga gagagtagat aagtaaagct
     1441 ccatatagat ggtagtttat aatttaattt gtttatagtt agtgaaggta cgagatatcc
     1501 tagaaaaata ccgagtataa taaaaattat acctaatata ccataccagt cctttacatc
     1561 ctgtattggt tcagatattc tctggtcatt taatatatca attatatttg tacgattaat
     1621 aaattttaca gaaagtataa atatgcataa tgtcacaaat acactaaata taaatccata
     1681 tatgaatcct tgagttccaa aatgatatgt catttctctg gtatcaataa tcactaattg
     1741 aaatattttc catataccaa aagatacagg aatacttaat ataagaccta aaaaacagca
     1801 tactactgaa attaacaata tctctgaaaa tagaagtttt tttaatttct tcttatctgt
     1861 tcctagagcc atgaaaatac caatttcctt gcttttaaat tttaaaaaga gggaagatgc
     1921 gtatgtagta aagacagtgc atcctaatat agcaactgag aatatcattg tagcctgttt
     1981 tctgctatct ccaccagttg gaagtatgtt ttgtattgtt gggctaaaat aaattagagc
     2041 atatgaagtt actaatacca cagaaagtat tatacaaaag aataaagttg tatagttctt
     2101 ccaattatag gctcgaagtt tactgtataa tttattaatt gtcatcttga gcgcctccca
     2161 tttcttttaa aacttctagt aattcatcta ggaatttatg tcttgaattt gattttacaa
     2221 cttctttata aattattcca tctttaagca caataactcg gtcacagaag cttgctgcaa
     2281 agctatcgtg tgtaaccatg agtatagtag catctaattc ttgctttgct tttaaaaatg
     2341 attcaatgac tgtacgacaa gacttactat caaggttacc agttggttca tctgctaata
     2401 ttaaatctgg gttgttcatg agtgctcttg ctactgctgt acgctgtttt tctccaccag
     2461 atatttcggc agggtatttt tttctgatat gttcaatacc aaatagctca catagattaa
     2521 ttgcacggtt ttccatttgt aagacttgac tacctttgat aatttgtgga acacatatat
     2581 tttcaaatac actaagacca tcaaatagca tgaaatcttg gaatacaaat cctagttgtt
     2641 gatttctaac ttctgaaagt tccttttctt ttaaatttct tatattatta tttccaagaa
     2701 ttatttcccc attatcaatt gggataaaac atgaaataca atttaaaaga gtagtttttc
     2761 cacttcctga tgaacccatg attgcgacaa attctccttt gttgatttca aaagaaatat
     2821 tttttaatac aggatatgtt ttactgcctg ttttatatga tttacataat tggtttactt
     2881 taagcatata tatcatcctc actttcattt atcttgtaag tcaatgatag catttgaaag
     2941 gggaggaggg atatgagata tattgtaata gttggatgtt tttttgtaat gtttgaaaat
     3001 atattgtttg gatagtgtga gaggttatta ttatagttaa attgattata ttattggagg
     3061 atttagaggg ttatttttag gtattaattg ttgtgctaaa gcaatatgat gtggaacatt
     3121 gaaggtgtag ttagaaaaga ggtatatagt aaaagtaaaa aggttataga ataacgaatg
     3181 ttgtattgaa aaaaatgatg taaataatgt atagtagggt taaaaagaaa aaaagaatta
     3241 tagagcagta agattattta atggaatgta ttgtaaataa tttgaaagga tttaggggtt
     3301 attaacacaa tgtaattgag aaaatatatt ggagttttgg aaataaatag gttgttaaaa
     3361 acaattgaaa tgtataataa aaaggttaaa aagtattatt agtataggta tgatagataa
     3421 ttttagaaag tggaagtatt ttagactatg ctgaaaagtg attgatatga ttttgagata
     3481 ttaaaatatt actatattat agtaaagaag ttaaaataat aaaatgttgt aataataaaa
     3541 gtctgtagta taataaagaa ctgtaataaa taagactttg caatgtaata gaaatgctgt
     3601 aataataaag gtctgtagta taataaagaa ctgtaataaa taagacttta caatataata
     3661 gaaatgttgc aataacaaaa caccataata aataagagtt tgcaatataa tagaaatact
     3721 gtaataacaa aacactgcaa taaataagag attgcaatat aataaaagtc tgtaatataa
     3781 caaaatccta taataaataa gattttacaa tataataaaa atcctataat aaataggact
     3841 ctgtaatata ataaaatgct gtaacaacaa aacactataa taaataagac cctgtaatat
     3901 aataaaaata ctataatata gtaaaaatca tgcaataata aaaatctata ataataaacc
     3961 ccagcataac aaagacatta atttcttact aaaaaggcca ccacaattaa cagtgaaaaa
     4021 gcaattgaaa gtattgaaaa agtaaagtga gaattagagt cattattacg attattaagt
     4081 tttatccata aaccacatat aattgtacac aacaataaaa ttgctgatat tactcctata
     4141 aatgtaaaaa acattaaaat tcctccccct tatttaatgc aagtattttt agtcaatgag
     4201 aatattttca aacgttacca taaaataaaa aaatgtacat aaaaaattaa tatagtatta
     4261 ttttgtaata tctaccaata ataaatctaa ttgaaaatct attaaaccat ttctctgttc
     4321 aacattaaga atatcaatac cactatactt gaaaaaagca ttagaacgaa gaaaaagcaa
     4381 ctgaataaat gaagttattt gatacgcaat agttctgtat tcagcttcag tttttttgtt
     4441 attctgattt tcatagtgag ccttaattaa aggtagtatc tcaaatggat aaacaatttc
     4501 ttcctgtatc aatatatagg gtacagaaat ctttataaat tgactatgtt cacctataaa
     4561 atcaaaaaat gcaattagta tttcccttaa ttgctgttta ggtgctaaat ttttagtatc
     4621 atgtgtagcc ttatattcat ccatatgacg ctttaaaaat tttttaatag aaatattaat
     4681 taactcctct ttagatttaa aataataatt aatcatagct aaattgacat ttgctttagc
     4741 tacaatttct ctagccgtaa tgcttgaaac atctctatgt gaagatagta actggattgt
     4801 agcattaatt aattgttcct ttgttgaatc tgatttcata atttttcccc ttaaaaattt
     4861 ccccataaaa aaattaaaca tgtttaatac acgtttagat actatcatga aaattttggg
     4921 atgtcaatgg gtgattagta cttaagtatt taagtactaa tcgattagac ttttataagg
     4981 gggatttgaa tttctgtaag ataattttca ggatttattt cattatgttc acctttatga
     5041 ccgatttgtc tatttatacc tgatatttga taatcactat ttttatctaa ccaataacag
     5101 aacgattcat atgcaccagc tatattttca tatggaccat aaaccatagt acatgccatc
     5161 atatcaattt cttctgtctc acgatatata aatccagatt tgttctttcc aatcttcttt
     5221 acaaccattc caacttcaat atctacaccg ttatctttga cttcttcatc atggtaaaaa
     5281 gctatgttat tgttggattg acgtgatact tctatatgtt cttctttgat aaatttagat
     5341 agcttttccc agagtatacc ttcagattgg tagtctggaa ttatctctct aagtgatatg
     5401 attttataac ttggaatctt tttaaagaca acattgtaat gaatttcatc tttatcacag
     5461 tttatagctt ctataaattt atcaatttta tttattcttt gttgttcctg ctttatttcc
     5521 ttttgtattt cattcttttt tctattcaat tccttaatta caaattcatc attccaatta
     5581 tgtacgatat ttgctatttc tgcaacgcta aattttgaat ctctcaataa tacaattctt
     5641 tgtaatattg aaatctgttc aactgagtat aatctatgac ctgtatattt gtctatttct
     5701 gcaggcttta aaataccaag ttcatcataa taacgcaaca tacgaataga aatttgtgtt
     5761 agttttgaaa cctctcctat tttaaacatg aaaatatgac ctccttttca cttataatca
     5821 gtatatattt tgtttttata tacttcaagc gtttgtggaa atacaattat tttccagatg
     5881 ttgcaataaa ttatagagag taaattatat ttaagtagta atcgtatcaa atcatagatt
     5941 atattaattt ataattcata ttaaaattac cagtctaact caaattcaaa atcatccgac
     6001 tttatctgtt caatttcaga atttaccttc tgtttaaatt tattagaaga aatcatatat
     6061 acaagaaata ttaaaccaaa tattatttgt acatattgag ctattacaca taatttaata
     6121 gggtagtgta gaagtaattg aatgttgaaa aaatagtaca tcatatgaac taatccagct
     6181 gataaaatgc ttctacttgt attgatatat acaaaattaa ttataaagtt taaaagtaca
     6241 atgtttggta taaataaaaa agcatcaaat agggagtatt gtgccaaatt atatggtatt
     6301 tgattaggtg taaaatacga ccctagatac cagattccac aaacaagccc taaaataatt
     6361 gaagagccta taaatccaaa tctaactaat aatttgtcaa tggcatatcc tctccatcca
     6421 gcttcttgac taagtatact caatataaaa cttgctaata gcatataagc tattttagaa
     6481 ggtctaaaca ctgctatgtg tatccaatct atatcagaag tttttacatt aaagtaattt
     6541 ccgattatta acgaagtaat aaaagttaaa gcaaaaaata atattgttaa tagtatccat
     6601 tttattccga tttctttaaa gcttaggtat ctataaataa aatcttgttt tgcactttgt
     6661 ggatagattg tagaaataaa aaatagacct acaatagctg gtgatgtata tgcaagataa
     6721 aaaatgattt ctccaagtat tgtatttgta attccgagca acgcaggtgt aagactgaat
     6781 atccagttca atactagagt aaatgtaaag aaatagatta agtgtttgtt tgtattaaag
     6841 ggggtgttat atagttgcat tttttcacta tccttccgtt ttataatgaa gttttaattt
     6901 ttattaattg tttatttatt tttatatgta ttaagttatc gcttgatgtt agttattgcc
     6961 tataataata tcctattttt tatattttga ttaattattt ttacactaaa tataattaat
     7021 tagtatttaa tattgaatat attatattat tattaattat taaataggtc aataaatttg
     7081 caattagtga tattaataaa ttactttaga tttttattaa atgtttagct tttctttaat
     7141 attttttata taacgacgat ttataataac tttttcgttg ttttttaaag tagcttgcat
     7201 gcgattatgt gatattactt ttatactttt tatgtagtcc atatttaaaa tcatggattt
     7261 agatattcgt gagaaagatg tattagctaa taattttgat aatttatata tttttaaatc
     7321 aatttcaaat acatcatctt cataataagc aaatgtttta tttccaactg tctcaaagta
     7381 taaaatatca cagacattga gtttataaat ctctcctgat tttttacctt ttatatcaag
     7441 taacggtata ctcaataatg aaattatttt ttcaatgtta ttatctagag cagaacattt
     7501 tatggttatt tcaatatcat cacaattaat accttgttcg acatgtattt tcattaatca
     7561 tcacctcatt ttattactgt cattagaata gcattatatc ttattgagtc caactagatg
     7621 cgttctaaat ttcaaattaa gcatagtgaa attcaaaaat aaaacagtga aattcaaatt
     7681 tgaggtggta aaattcaaaa aaatactatg gatatttgga taaattatcc aaatatccta
     7741 gtatttcaca tagaatatgt ctaattattt tacaaataga cctattttat gacttacata
     7801 tattgtcaaa ttacttctta aatatcaaaa tatttttgga taatgtatca atactgtttg
     7861 ataaagtatt taatttaccc tcaattctaa ccaacaagta catggataga gctataggaa
     7921 aaccaacatt ggttattaac agttgtaaac tatcatccat aaaattagcc ccctattcta
     7981 taactaagtc ataaggagtg gtatttgttt ccacaacctt ggcttcaaca acagaagcta
     8041 ggtcaaatcc tccaaccgaa aaaatatttt tagatactat caaatccatg acagatttta
     8101 tattttcttc agttaaatca tcctttggat tttctataga aaaagaaact tgtttatcct
     8161 ttacattttt aaagctcatg actaattttt tcttaatttc catataatta cctccgatat
     8221 aataatttat attttaattg tttgcttgtt aatattggta ttttaattat caatattaat
     8281 gttttgcttg ttagtattaa tatttcaatt gtcaatatta atgttttgct tgttagtatt
     8341 aatatttcaa ttgtcaatat tagtgtcttg cttgttagta tcagtgtttc aattgtcaat
     8401 attagtgttt tgcttgttag tattaatatt tcaattgtca atattagtgt tttgcttgtt
     8461 agtattaata tttcaattgt caatattagt gtcttgcttg ttagtattaa tatttcaatt
     8521 gtcaatatta gtgttttgct tgttagtatt agtatttcaa ttgtcaatat tagtgttttg
     8581 tttgttagta tcagtgtttc aattgtcaat attagtgttt tgcttgttag tattagtatt
     8641 tcaattgtca atattaatgt tttgcttgtt agtattagta tttcaattgt caatattagt
     8701 gttttgcttg ttagtatcag tgtttcaatt gtcaatatta gtattttaat tgttgactct
     8761 agttattgct gatatagaaa atatttaatt tatttcaatt aattagatta aagtagggta
     8821 gcatctaaaa tagctaacat gtttaagttg aaatacgttt aatatattaa tttttcaaag
     8881 cgttaatata ttaaagttat aaccaaaatt gaagttataa tctgccaaat ctatagcgta
     8941 ttaagtgcat tatataactt aatcttttat ataaagagtt aattttttaa tttgtaaagc
     9001 agagtacgtt taaaagacac tatttagata gtgatgtact atctaatttt aaaatatcta
     9061 caatagggtt ctcttgaaga cttgctattg cttctgcaac agcataaaca tcttcattta
     9121 atgcctctgc ttttacattt gcaaatgatt tacttttaac aactgcctta cccttatcat
     9181 caaaaccagc atccaatttt agttttatac tagataaatt ttttgttgaa acaacttcca
     9241 taaaattacc tcctaaaaat ttaatttcaa agatttgtcg actttctttg ttagctataa
     9301 tactatcatc taagataata tcggagttaa acagtaattt ctaaagattt tttgtgtaaa
     9361 tttttatagg aaaaatttta cttttttgct gatgaaaaag tttacaagtt atttgtgaag
     9421 atatgttctt agtgttttga aatagtattt gtatatattc atgcttagtt gagatagttt
     9481 tgtatggatt ttcatatgtg tagagttaaa tgttaggtac taaagtttat gttaaattaa
     9541 atgttaaagc ttatgttaaa aattttatta aatttaatct gtaaataaaa aagttaaaag
     9601 tccaatatta acattttact tttaaaccat taaaatcttt attaaagcta acaaagttat
     9661 aagttatgag aaattttatt gaagttaata caaaaattaa ttttaaagct aagaaatttt
     9721 ttattagatt caataaaact aataaatata gtttagtcaa taatgtatta aataaaaact
     9781 ttgttaagtc tataaaaata tcttaaagga ggtgtaactt atgaagataa aagtggagga
     9841 tataccagtt caatttcatg caatggtaga aataatcgga attgataagt tcatagaggt
     9901 tgccaagttg tatggaggaa caaatactta tatacctacg tataaggggc tttttagata
     9961 tgctagaaat agggatatag taaaacaatt taatggagtc aaccacaatg aattggctat
    10021 tagatataat atgtgtgtaa gcaatattaa gcgaatactc aatgaaaata gtgtttaaat
    10081 ataataaaat aatgtttaaa cataatgaaa atagccttgt taaacgtaag agtaaagaca
    10141 aagaagatgt tttataaagt ttttttagac aaattaacta aatttactac ttattaattt
    10201 aaatacattg tatattctat aaaaaaattt gttaaatact ggagatatat tgagaataca
    10261 aagaaaatgg tcgaaaatat ttgttggcta aaaaatagat aaaatgatac aattgcttgt
    10321 ataaagggga aaaataaata aataaactat ataaataaag aaatagcaat taatatgaca
    10381 taatatattt ataaaattaa gaatataaat agatatttta agggattagt tgttaaatta
    10441 ggaaatatta aaaaaaatat atatttatat ttaaactatt ttgttattta tataaaaata
    10501 cttatctagt tactagcaat tattagtaga aatgggggaa atagagatga ataagggatt
    10561 ttacaaatta aattcagtgg tattgaactt tgatattaag aatgatgtta acaagataag
    10621 atttaatcat tatataatga gaaaagaaaa catacaaaga cttcaaggaa ctctgccaaa
    10681 cggacaattt tatatgacag tgagaagagc tgctacagat ttacaacttt ctatttctac
    10741 tataagtagg cttgtaaaag agtttttgga tttagggata ataagattag tgtctaaggg
    10801 tgtaaaaggt aatcgttctg tatatagtta cgtaagtata gagtttaatg gaagtttatt
    10861 tgatgatagt aaatatatgg aacttgactg tgtatgtaat gatgaattta ttgataatag
    10921 tggcatagag tttgattgtg atagcaaaga taattctttt aatggagata taaatgtaga
    10981 aactgtgcat tgcattaatg agtatttctc tgatgatgat gtagagcata attataacaa
    11041 tttccataac aaagatacta taaaggataa agccatttct aatattagca ctatcaaaaa
    11101 atatattgat gaatcttttg atacaaataa atcagaaaat tctaacttac aacaggagtc
    11161 aataaatttc aactttaatg atacatgcaa tgaaatagat aaaagaacga ataatagtat
    11221 agaaaataat caaaataaaa ggcctatgag attgagtgac atcagtcttt taagtgataa
    11281 aggtatgaca aataaaaaga attactttaa tcataatcat ggtatacaaa aagggactaa
    11341 atctgtaaca aagaaaaaag aatccttaaa taaatttaat aaaaaaaata tacagcaaag
    11401 cattataaat atgctaaata aaaaatcagg taagaatttt aaaatcgatt ctccaataag
    11461 cataaaactt atcaatgaaa ggctaaagga aggctatgtc ttagaagatt tctacaaagt
    11521 tattgaggta aaagtggcta agtggaaaaa taccataatg gaaatgtata taagaccaga
    11581 aactttgttt agctataagt ttgaatccta tgtaaatgaa aacatatcta tgaaaagtca
    11641 aagtaatgtt cacccatgta aagaaggcaa tagtaatgat agcagtaatt atatacattc
    11701 tgattatata acatatgatg gcagtgtgcg tgagagaaaa aaatatgaga atgtaagata
    11761 ctggaatcct atatgtgaaa tttaattagt ttaaaaatgt atttatgata aataagatat
    11821 taatagagaa tagtgagcta tagcaaatga tatatcaata gttggtagta agttatatta
    11881 agtaagatat tactatcagg tagtgaatta tattgaacga tatattaata attggcagta
    11941 agttatatca aataagatat tactatagag taatgaatta tattgaacga tatattaata
    12001 attggcagta aattatatta aataagatat tactatagag taatgaatta tattggacga
    12061 tatatcaata gttggtagta agttatatta agcaagatat tactatcagg tagtgaatta
    12121 tattgaacga tatatcaata attggtagta aattatatta agtaagatat tactatcagg
    12181 tagtgaatta tattgaacga tatattaata attggcagta aattatatta aataagatat
    12241 taatattgga tagtgaatta tagtggacga tatatcaata attggtagta aattatatta
    12301 agtaagatat tactatcagg tagtgaatta tattgaacga tatattaata attggcagta
    12361 aattatatta aataagatat tactatcgag taatgaatta tattgaacga tatatcaata
    12421 attggcagta agttatatca aataagatat tactatcgag taatgaatta tattgaacaa
    12481 tatattaata attggcagta agttatatca aataagatat tgctattatg tagtaaatta
    12541 tataaaacaa tgtatcagaa taaatagtat attaataatt tgtagtatat catgacgaat
    12601 aatacagtca taacaaatag tgagtcataa caaataatgt atttataatt gataatgtat
    12661 ttaaaaatca aaatataaaa acttataagg aaagagagat taatttatat gggtaaatca
    12721 aaagatgttg aaatagagaa gtctatttta ggcacaattc ttttagacaa taaattaagc
    12781 tataagctag atgaattaaa tgaaaacatg tttatgaatg atatatgtct tgagatattt
    12841 aaaattatga aagaacttaa aaaagaaaac atagtgattg atgtggccac tgtgaaatca
    12901 aagatagata gaaaatctct agcaataaaa acaagtgatg tgactaatct aattacttgg
    12961 ggtcagaatt ttggtcttga tggtcatatt aaaattttaa aagaaaattt agctagaaga
    13021 agcataaatc aaaactgcca aaacctttta cacagcctaa accttggaga aaatatcgat
    13081 acttgtattt acaaatttga aagtaatata aaggagattt tggacaagga tacttatgaa
    13141 aatgatgatg taaatagtat agcagggaag gtgcttgatt ttttagaaaa caaaaaagat
    13201 attggattta aatttggtat aaaattactt gataccacta taggaggtct ttttaaagga
    13261 gagctaacca caatagcagc taaatcgggg gttgggaaga ctgctttggc acttcaaata
    13321 atgttaaatt catttaagca aggtaaaaaa actttattta taagtagaga aatgaccagt
    13381 gagcaagtat ttatgagaaa tatatgcaga gtaactggtg taagtacaag gaatatgaaa
    13441 tctaaagaaa tagatgaaaa tgattggaaa cttattgtaa atgcgatagg tgatttaagc
    13501 gaaaataact taatttatat caatgataag atagatacaa ttagtgcaat tagaaagagg
    13561 ataaggcagg taaagccaga tttattaata gtggattatg tgcaactcct tacatctcag
    13621 aaaagcatgg ataagagaga aagggaggta gctacttttt cgagagaact taaaaatatg
    13681 actttagatt ttaatatacc tgtaatccaa ttaagtcagt taaatgatga aatgaaagat
    13741 tcaagacctt atggagatag acctatgagg gatagcaaag ccatctacca cgatagcaac
    13801 aatgtggttt atatacatca attaaagggg agtgattatg aagaagcagt gagagatatt
    13861 ggagaaagtg aggaggcagt tagagcatca gaatacaggg gaattaagat ggttgattta
    13921 atagttgcga agtgtagaga tggacagact aggcataagc atttttgtta ctttggggat
    13981 aagttacatt ttcaagaact aaattattag gtcaataaag tagtaataac aagcttatta
    14041 aaagtcggga aatagcaaat agattataaa atatgaggtg aaaatatgtt ggaattaaga
    14101 aaaattatag aattacttga agaacacaag atagaagtaa gtattccaaa ggagttacat
    14161 aagtatagtt tattggaata ccttgaattg tgtcaacaaa gtgggggaac atatttttat
    14221 attaaaacac tggaaggggc tttaaaagag gctagaaatg agcttatgat taaattgttt
    14281 aatgggaaaa attatatgga attagcgagg atgtttgaaa tgagtgttat taatgtcagg
    14341 catattgtta gaaaaaaata gatatttttg gagaatacaa gtgtgatata cttctgaaat
    14401 agaagttatt ataagaggag tataagtgtg attaacaaca tgattttgtg aataaaattg
    14461 aggatatata tgagttaaat gtgtgattaa taaggtaaga ttgtgtataa gattatgagt
    14521 atttaaaagg aggggctata attaatagtg tagagttgtg tgtttgctta agattatata
    14581 ataatttaat agattaaact atactaataa ggtaaaatcg tgtgtttagt taaagcttat
    14641 tataaaatct tgatatgtga ttaatgatgc aatatgtgaa taaagttaag attataatag
    14701 atttaacctg tggctaatag aataaaattg tgaataaagt taagattata ataggtttaa
    14761 attgtgacta atagagtaag agtgtaaata gagataaact tcaatatgag cttaacctat
    14821 gattaataga gtaagaatgt aaatagagat aaacttcaat atgagtttaa cttgtggcta
    14881 atagagtaag agtgtaaata gagataaact tcaatatggg tttaacttgt ggttaataga
    14941 gtaaggtgta actaaagata aacttcaata tgagcttaac ctatgattaa tagagtaaga
    15001 atgtaaatag agataaactt caatatgagt ttaacttgtg gctaatagag taagagtgta
    15061 actaaagata aacttcaata tgggtttaac ttgtggttaa tagagtaaga atgtaaatag
    15121 agataaactt caatatgagc ttaacttgtg attaatagag taagagtgta aatagagata
    15181 aacttcaata tgggattaac ttgtgattaa tagagtaaga gtgtaactaa agataaactt
    15241 caatatgagc ttaacctatg attaatagag taagaatgta aatagagata aacttcaata
    15301 tgggtttaac ctgtggttaa tagagtaggg tgtaactaaa gataaacttc aatatgagtt
    15361 taacttgtgg ctaatagaat aagattgtga ataaagttat agttttagat gggtattaac
    15421 ctgttactaa tagaatatga ttaggtgtaa aattatagag ctgcataaga aaatattata
    15481 gatatattaa atattagata ataaatattt atatagatac acaaaagaaa aggcgatatg
    15541 aagctaaact taatgacttc atatcgtttt ttatttgaaa ctcctttgta tttagagtgt
    15601 gatttttaat attgaggttt tatgtgatta agtcacagat aaaaagtaag tatcttgata
    15661 taattcttat agattaataa tatagggagg ttattatgtc taataaaaaa aagaagtatg
    15721 caaatatttt agcaaatgag tgcgttgctt gtggtagctg tataaaagca tgtccaagaa
    15781 gtgctatctc agttccatgt ggcattagtg caaaaattaa tagagattta tgtgttggat
    15841 gtgggatttg tgaaaagata tgtccagcat ctgtaattga gattataaca atattaaaag
    15901 aggaagaagg taagtgtcat gaataataaa aaacattggt atgactattt atggattttt
    15961 tcttcaatat atttgatatt gggatttgta aatattattt tcgcttggat tgggctaatt
    16021 tgtttctttg ttccactagc tatttctatt gttaagggaa ataaagctta ctgtaataag
    16081 tattgtggaa gagggcaact gtttaatttg cttggaaata aattgaagct gtcaagaaat
    16141 agagatattc ctaaatttat tagaagtaag tggtttagat atgggttttt gacatttttt
    16201 atggtaatgt ttgccaatat gcttttttca acttatttag tgtttgaagg aagtagaaat
    16261 tttaaagaag tagtgacttt gttgtggact ataaaaatgc cttggcaatg ggcgtatcat
    16321 ggaactttag tttcaccttg ggtggtacag tttgcatttg gtttttatag tgttatgctt
    16381 acatcaactg ttcttggttt gattactatg atattgttta aacctcgttc atggtgtgtt
    16441 tattgcccta tgggtactat gactcagatg atatgtaagg caaagagtaa gtctgtatag
    16501 aaaggcctta tgtgatattt acagttttgt tcaagtagag atgagtagta ggtttgaata
    16561 tatatgacaa atatatgagt ataggggcat agatttagtt tgtgtaaggt aaactgattt
    16621 atgcttcttt tttatgtctt agattattgg ctaagtggaa acaatgaatt tagatattaa
    16681 gaattattca gttatttgaa gaaaatagaa taataattaa cattatatta atggatattc
    16741 attacatata agccagttaa tagcaagatt tatttaatat gatataggtc aagtatttaa
    16801 actaaataag gtcatgacaa ttaatataag tatagatata aaaagattag aagttataac
    16861 ttataaaagg tattctatat aaatagtatg gggaaaatag tcaaattaac ttattagaaa
    16921 caaaaaatat acataaaaaa taaatgtaaa tatcaaataa gatactatat caaaatagat
    16981 ataagtatct tttttattat tgaaattatg aaaatatatt tgattttaca caaaaaagag
    17041 tgcctcatga actaaaaagt tcattttgag acacactttt acagtgtcaa agaacgaagt
    17101 ttatccttat ccaatacctt tataccacca cgagataaag acacaatacc ttctctagca
    17161 aaatatttca acattctcga tacaacttct ctggcactac ccatatactt agcaatttgc
    17221 tcatgagtca tatttatagt gctactgcca tttttagcta tctcatcaat taaaaaagat
    17281 gccaaacgct tatcaaaact cataaaaaga atttgttgca tagcccacat aacatcagaa
    17341 aaacgttcag tagctaattt atatgaaaat aactctgcat gaatattttc tgtagataat
    17401 tttgcaaaaa cagaggaact aatctgtata atatcgcagt ccgtttcagc atctacatag
    17461 acatcaaaag ttattgtttt tagaatacag gaagctgata aaatacatac atctccatca
    17521 tcaagtcgga acagtgtaac ctctcgacct tcatctgaaa gtatgtatgt acgcactgtt
    17581 ccagatttta taatcaaaat accaatacag tcactatcac cacaatgtac tggattccct
    17641 tttttgtaat gcgaggtatt tgctgaagaa attaataatt ctttttgtaa gtcagtaagt
    17701 ttatcccaaa aaggcaagct atgtgtaagt atatcaattt cattttgtga aatcatacaa
    17761 tatctccttt cttaaattgt tataatccca tgatacaagt gaatagataa attgacaagt
    17821 agtttgttaa gaggattatt tagtcagata gtaatagtat gatttatgta gaaaaaatat
    17881 gataatatat attcttgcta ttaatgagaa agtaatatgt tttgatgata gtaggtttgt
    17941 ataactaatg cataccgttt atttttaaaa gattaagttc cttatgtaac cttatagaca
    18001 taaaaccagc aattatagca gccatcaaat cagctattgg ttgtgcatat aagataccat
    18061 ttaacccata gaatacagga ataatcaaaa ttatagggat aaaacagaca ccttgtctac
    18121 acatacctaa aaatccacct tcctttgctt ttcctagtgc gaggaataat gtagaataca
    18181 ctgtatagaa tccaaatagc ataaatgaaa acccatttgc cctaagagct ttactaccat
    18241 tttctattaa taaaatgtca ccacttgtaa attgtgaaat tatagatttt gggaagagta
    18301 tcaatataag accaagcact acacaaaata ctgtagtcca taatatggaa gtttttattg
    18361 ctatatgtaa tctatcgtag ttctttgaac caaaattata accagcaatt ggttggaaac
    18421 cttttataaa accaaataca acaagactac ctaaagacgt tattcgtgtt acagcaccca
    18481 ttccagctat cacagaatca ccatactttg tagcctccat attagttaat gtaatagcaa
    18541 gactagtcaa taattgaaat attaaggtag gtatgccaat ttttaggatt tctgaaaaaa
    18601 tttcttttga aaaacagcat tcgttaattc taaaactaaa tatacttttc ttgcttaaaa
    18661 tatatcctac ataaacaagt gttgaagcaa tctgtgagat agctgttgca atagaagcac
    18721 cccttacccc cagaccaaaa gtataaatga atatcgggtc taaaatcata ttaagcaaag
    18781 cacctaataa catagcacac atagttgttt tagcagctcc ttcacttgtg acaatattag
    18841 tcatagtgac attgaatata ttaaaaatag atgacacgac ataaatgcta gcataagata
    18901 ttgcataagg cataatactt tttgtagctc ctaacaattt taaaattgga ttgagaaaaa
    18961 tcataatcac aataataact aatgtaccca ctataatact actgtaaata gatgtactta
    19021 ccacttttcc tgctttttca ttatcaccat ggcctaaaag tctggcaaca tatgaagcag
    19081 ctccatttcc aaacaataat cctaatccca caacaagctg acctaatgga aatgcaacgg
    19141 atatagctcc catttgactt gttccaagtc cacccacaaa gtatgcatca accaaattgt
    19201 ataatgcagt tatcatcata cctatcatgg taggtaatcc catagataac aatgcttttg
    19261 agattggggc agaccctaat aattcaattc ttttgttact ttctttcata cttaacactc
    19321 ctcttctatt gacattaagt ctaaattata atactataaa ttatagtaag tatggaaaat
    19381 agtactataa tttatagtat ttgtcaatag taagaaaact aaaggagatg atttttatgg
    19441 ctacaataga tttaattgta ttgggaatag taaagcaaca acccaagagt gcttatgata
    19501 ttcaaaaggc agtggaatac aggaatatat cacgttgggt taaaataagt acaccatcaa
    19561 tttataaaaa agtaattcaa ttagaacaaa agggatatat tgaaagtaaa actgtgaaga
    19621 atggaaaaat gccagaaaaa gcagtttatt cattaactga attaggtgag acacattttt
    19681 tagatttaat gtttgataca tctagcaaac caataaatat tcttttagat tttaatgctg
    19741 ttatagtaaa tttaaatagc cttacaccag aaaaacagaa attatgtctc aataatattg
    19801 aagagaatgt tcaagagtta aagaaaacta ttgaggaaaa tataagtata aagacagaag
    19861 taccagacac agcaaaagca gtgttggagc aacagttggt gctagcacaa gcattggaaa
    19921 cctggataaa ttctttaaaa gataatatgg gaaattagaa ttagagaagc taacttaata
    19981 atcattaaac aaaaaccagc atatttaata atgagatatt agaaaaaata aaacaaatga
    20041 tataaactga aaagtatatt taaaataaag ctcacttaat tgagggagaa tgttaaactt
    20101 tt
//
LOCUS       DAMJYC010000071        20037 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJYC010000071 DAMJYC010000000
VERSION     DAMJYC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.072"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            160..615
                     /locus_tag="PC472_002734"
     CDS             160..615
                     /locus_tag="PC472_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728050.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(823..1083)
                     /locus_tag="PC472_002735"
     CDS             complement(823..1083)
                     /locus_tag="PC472_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728051.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(1346..1699)
                     /locus_tag="PC472_002736"
     CDS             complement(1346..1699)
                     /locus_tag="PC472_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728052.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1716..7037)
                     /locus_tag="PC472_002737"
     CDS             complement(1716..7037)
                     /locus_tag="PC472_002737"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728053.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDSKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(7054..8037)
                     /locus_tag="PC472_002738"
     CDS             complement(7054..8037)
                     /locus_tag="PC472_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2728054.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(8038..8736)
                     /locus_tag="PC472_002739"
     CDS             complement(8038..8736)
                     /locus_tag="PC472_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2728055.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8733..9785)
                     /locus_tag="PC472_002740"
     CDS             complement(8733..9785)
                     /locus_tag="PC472_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2728056.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYLRDIRGEIIYTKVMGILINTTGVHDLSKLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9778..10206)
                     /locus_tag="PC472_002741"
     CDS             complement(9778..10206)
                     /locus_tag="PC472_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2728057.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(10206..10532)
                     /locus_tag="PC472_002742"
     CDS             complement(10206..10532)
                     /locus_tag="PC472_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2728058.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10548..12077)
                     /locus_tag="PC472_002743"
     CDS             complement(10548..12077)
                     /locus_tag="PC472_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2728059.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGIVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(12359..12781)
                     /locus_tag="PC472_002744"
     CDS             complement(12359..12781)
                     /locus_tag="PC472_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728060.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12781..15234)
                     /locus_tag="PC472_002745"
     CDS             complement(12781..15234)
                     /locus_tag="PC472_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728061.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15234..15401)
                     /locus_tag="PC472_002746"
     CDS             complement(15234..15401)
                     /locus_tag="PC472_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728062.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15428..15874)
                     /locus_tag="PC472_002747"
     CDS             complement(15428..15874)
                     /locus_tag="PC472_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2728063.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(16000..16428)
                     /locus_tag="PC472_002748"
     CDS             complement(16000..16428)
                     /locus_tag="PC472_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2728064.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16442..17506)
                     /locus_tag="PC472_002749"
     CDS             complement(16442..17506)
                     /locus_tag="PC472_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2728065.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17511..17954)
                     /locus_tag="PC472_002750"
     CDS             complement(17511..17954)
                     /locus_tag="PC472_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728066.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(18203..18643)
                     /locus_tag="PC472_002751"
     CDS             complement(18203..18643)
                     /locus_tag="PC472_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728067.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18748..18945)
                     /locus_tag="PC472_002752"
     CDS             complement(18748..18945)
                     /locus_tag="PC472_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728068.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQS"
     gene            19114..19434
                     /locus_tag="PC472_002753"
     CDS             19114..19434
                     /locus_tag="PC472_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2728069.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19516..20022
                     /locus_tag="PC472_002754"
     CDS             19516..20022
                     /locus_tag="PC472_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728070.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         6235 a         3549 c         2100 g         8153 t
ORIGIN      
        1 cttttttata attttatgat aaaataatta gtatattttg ctttacttga atgtaattat
       61 ttatacaaaa gtcaaacata atataaatgt agtaaatttt tagtttcaag taatatctcg
      121 ttattataac taaatattta ttaaaattag gaggataaaa tgggcttaag agacaaattc
      181 gcgcaatcat ttgctagatc aaaaactatg agtggccctg agaaaaaggc taatgaaata
      241 atgggtaaat tgcttcttaa gaaagctata ttacctatcg ttttaatgtt cgtgataata
      301 atcgctggag ctatgcttaa aatcaattct tgggttacac ttggcataaa tttagttatt
      361 gctgttggtg ctttcttcta cataagaaat tctagtaaaa agtatcagaa ctttaaacct
      421 tatgttggca atttgattag tttagagaaa aaaggtaaaa aagagtacgt tgctattata
      481 aaacaaggta agttaccagt taagttacaa atagcttatg gtggtgaaga ccttgaacat
      541 gttaagaaaa atcaaatggt tcagataagt tataatccag atgctaaaat agctattttg
      601 gttaatagac aataatttaa taaaaaaaag gagttgtctt aaaattgatt ttttctaatc
      661 aaatttaagc tcaactcctt ttttaatttt tatatattat ttttattact ttttttaatt
      721 aatattttta aatatcaaat ccttctcaat aaaatagtgc actatctaca atactacttt
      781 atcttttcct aacaatatat aatctggtgc tactctttca tgttatacac cgttttgtaa
      841 catacctttt ataccagtaa catcttcctt aatttcctct acatctgttt tcattatacc
      901 catttgaaca agtatatttt tgtttatttc ttgttgttgt gtagataatt ctataaaatt
      961 ttctacagtt tttttataca tgtctctatc ttcttttttc tcctgcatag tatttttaaa
     1021 taatagagca catagtatac ctattgcccc tagactactc aattcggtta ttaatttatc
     1081 cattatagcc tcctattgtc taaatttttt atacttatag ctttaatttt tgttgtattc
     1141 ttacatcatg caatattata ttttcaggat tatcaattta atcttctgta ctttaaattg
     1201 taggtttaat ttatagattg aataaataat aaatatatat acaatttcta atataacaaa
     1261 aaataagtac aaggcttact tattaaagtt aagaccttgt actttacata aaaaaaataa
     1321 ctaatacata gttattctaa atttactatt caactggtat ttctaattct ttacatctaa
     1381 ttctaacttg ctctctaatt actgctggta cttcttctat tgtttttttg cctcttaaaa
     1441 ttaaagctac atatacttct accattccac tctcacctcc agattttctt acactaaaag
     1501 tgcttatatt acttgcttca ttgacagaac tttgatttaa caacatctca tatatttctg
     1561 catttgctat aaaattattt gttatatcat catcttgctt tacttgacct atacttatgt
     1621 ctgaaatgtc tttatactgc atttctactt ttctaccttg tttgatttgt tctattttat
     1681 ttttatcgaa tttaaacaat tttcacacct ccattttaag cttgtggctc gttgttttca
     1741 gcatcatatc taaaaatcat agatggacgt tttatagctg tccatttatc tcctttttct
     1801 actatgattt taggctgagc actttttatg ttggcttctt ctaattcata gatttcttgg
     1861 taacttgagt ctgatttttt gatttcaact agtttcatgt ctttgaataa gtcagattta
     1921 tttattttta cttgaacttt agttttattt attaattgat atataggatt gttttccatc
     1981 atatcatcct ttaatccctc atttaatgtt actattaagt cagtgttatt ttcagtaatg
     2041 tttatgattg aatatacttt attgtttatc attaaaatag aatccttttt tactttaagc
     2101 cctgtttctg atttaaatat aaacatactt gaatataaat cgcctgtagg actactggct
     2161 ttaatcagta tcttattttc tcctaatact attttatttt gtggtatctc aaatataatg
     2221 ctatttattt ccaaattagt cttagtagat attaattcat catttataaa catttcaagt
     2281 ttttctataa cattattagg gtcttgtact acaactctca cttgagatat tgatttttca
     2341 actatttcta tagatgcttt acttgtatct acttttgtta tcatgtatat tgtatcatct
     2401 gaaagctcta tatctaaaat atcatttaga tgttcaggaa ttggtatttt agtaaattca
     2461 tataaatcag taattgatat agatatttta tacatagatt tatttttacc agttatccat
     2521 agactattat catttttaat tactagtatg tggtcatcat tatgtgactt aatttcttta
     2581 acattagtta atactttagt aaaagttctt attatcttat ctttttcact ttctcctagt
     2641 ccaaataatc catttgtatt gtacccttga gcgtacaact ctccctcatt agttaaaaca
     2701 agtgtcattg agttatatat acttatatct ttaacagaag gtaatccagt aaccttttgt
     2761 aactctctta catcattatt gtggccaact cccaattggt actcagtatt ctttccatat
     2821 gaccatagag tattatctgt cttaataata taggagtagt tatctccaca agcaactttt
     2881 ttgacatctg atatatttac ttcagtaaga gttaataatt ctatatcttc aagggtttga
     2941 ttaatttctt tatatgaatt acttcctatt ccatataact tgccatcctt agcaacagcc
     3001 aatgaatgtg tttttgatat atcaatatca gatacctcaa atggcaatat tattgaagta
     3061 tacttaaaat tatctttaaa tatgttttta taatttatat acttattatt tgtttttata
     3121 tacttatcat taaacattac tagcttttca tttttcttat taaaaactat tttatctgat
     3181 gtgttatctg ttttaaaata tgaatgtagg gaaccttcaa gagcttcatt tttatcatta
     3241 tcatatctat ggtgaatacc taaatgatta tatctattat ctcctgttga agaataaaat
     3301 tttccatata tatctttaat aaatatagta ttgtaactag aaaatacagt atcaacatct
     3361 ttctttatag atactttata atatggaact gacatatccc tataagattc atcagatata
     3421 ccataagtat ctctacctga tgatatcaaa ttgttattat tatctatata aagtgtatta
     3481 ttagctgtta caaaaacatc tttaacatct tccacactat ttgtattatt attatttgct
     3541 atacttccat atccactaag acctccaaca aatagtttat tgtcatttgt taaaatggct
     3601 gaagtatatc cattaactac tatttttttt gcatttataa tattattaat tactcgtatc
     3661 ccctttatat cattagtctc tgttgaaaag aataccctct cttttttccc tgtagtataa
     3721 atcatattgt catttgttat aaaaaatgta gcttcacttc ctgctgtcac aacagatttt
     3781 acattctcaa tatttatttt tgtaaatgta cttatacttc cttcagtaaa cccaaaatag
     3841 ccatttacaa gtccacatac atagcatgta ttatcatttt taacaatgat tgatacatca
     3901 ttacatagat atacatctct aacaccattt ataggtatac gtgtaaatat attcctactt
     3961 gtagtatcac ccaatcctaa ctgtcctttt ccattattac ctgctccata taaaagacca
     4021 tcattagtta ataatagaga tgtgtttcca tatacagcta catattttac attttcaaca
     4081 tttattttag taaatatatt tctatttaca ttatctccta ttcctaactg accagaatta
     4141 tttaaaccaa cagcataaca ggtattatca ttttttatcg caaatgcatg tttactattg
     4201 tcagtaaatc tatttacatt tatagatttt atattatcaa tatttatttt agtaaatacc
     4261 tttctattta tctcatctcc cactcctaat tgacctacat catttaaacc aacactgtaa
     4321 agcatattat tattagttaa taaatatgta gactttgaat ggctatatat ttcttgtata
     4381 ttagaagata tttcactact gctattcttt ttattgtaat ttatcttatt atttaaattt
     4441 aaactactat taactgatat accatgcgta cccttagagt tatatccaca tataaataag
     4501 ctcttatcag ttaatattgc aaatctagtt ttatctctac ttaaatcata aggaggtata
     4561 aatatatttt ttatattttt cattaccttt ttataattag tggcattata tccatcttgt
     4621 gtatctgaaa catctagttc ttttgcactt tcagatatat tcccaaaacc atatagcgtg
     4681 ttagaagata aatacaatag agctttatat aaaaatatgg ctttatcaga ggtaatagga
     4741 cctgaattta tattagatgc tgcaatatta ttagatgatt gattgttaag ataattcctt
     4801 ccatattgat gtatataaga tgaacctcca gaaataagta aattatctgg attgacattt
     4861 tttaaattac ttgacgattc cccattatat aaataagcaa aagttgtaga agatgatgat
     4921 tgtgtacatc ttgtaggata ataattatat atataagaat caagatatcc tgcatatgaa
     4981 attccctgat attgaactct atagcttgaa tcagttggtt ttgtccatga agtaccatgg
     5041 tatccagtta gaaaagatcc aatgtttgta caaaatacag ttacatactc atatatgata
     5101 atattattaa ttttagtaat attattcgtg catgaaactc tactataact tgaccctgat
     5161 atatttatat aataccaatc acttaataaa cctagattta cattagatcc atcagaagta
     5221 ctaaaaccta acaggtaatt tgtatttata ccagttgcat ataccttttt ttcatcaact
     5281 acaataaatg ttgcattata tgaactataa gcatcagtta cattactaag gctaatctca
     5341 gtatattgac ttattaatgc attactaagt ccattaacat aacttgaact ctttccagaa
     5401 gtgtatattt gcttattatt agttacaatt aatgaaaacc agtttgataa atcagtattt
     5461 ataacaactt tttttacatt atctatagat attttcttaa atacatctga attagaagga
     5521 agaacacctg ctattgagtt actatttggt ccagaataat aacaagtatc atcattttta
     5581 atgtagaata ttcctccaga aatatttttt gatgctacta tagactttac attagagata
     5641 cttaattttg tcaactcatt taattgactt ggtaagtcag aacccattcc tttgtatcta
     5701 ttatttccta gaccatatac atctccattc cctaaaataa ccagagtaca ataaggacta
     5761 aaaacgacat ctcttacaga atgtatatct agtggaagtt caatatcctt tatttccatt
     5821 tcttctttta ataaagttaa tttatcctca gatttaatta aatttatata attactataa
     5881 ctatattttt cactatccat tttcttaaat tctacaatgt gcatatcatc aaaaaacgaa
     5941 cctaattgtc cataagtatt atctcctgta caatatgaat cattatttga atcaattagt
     6001 atgctatgtg ataaaccaat gaaaatattc ttagctacaa tattttttga atctataaat
     6061 gaattatcta tattctcaga gtaatttgct atacctaact gatatatatc attatttcca
     6121 gcaaccttta aagtaccttt gttatctata agcatagtgt taccatgacc acatgcaata
     6181 gtttttatat ttacttcttc tatcttagta aatagtgatg attgaagtgc atttccaaaa
     6241 catttttcct ttccacaacc atatatatca ttattatacg tcattaacac agtgtgttca
     6301 gaaccacaag ctatagtctt aacatcatta atagaaacta aactaaataa atctctatta
     6361 ttcttatctc ctaaacctag ttgaccttga ttattaaaac cagtagaata gcatttattg
     6421 tcattcttta ttataaaagc tgatttatta tatgaaaata cagcctttac attgtcaaca
     6481 tctacttttg taaatgtatt tacatttgca gatgctccat tagttcctag acctaattga
     6541 ccatacaaat tatttccagc aacatataaa gttccatcat tgtataaaag atatgttgat
     6601 tgaagtccac atgcaatatc ctttacgcct tttatttctg gaaccttctc aaatgtagaa
     6661 actgttttcc ctactttttc taatcccaat cccatttgac catctgtatt tatacctgcg
     6721 acaagtaatt ctccatctga agtaagtata actgcatgag attctccaca tgcaactttt
     6781 ttaatattgg tagatatatt tggtattttc gtaaataatt ttcttgttgt attgtcttct
     6841 cccaatccaa gttgaaagtt actattatct ccacatccat acaagatatt attatttttt
     6901 agaccaaatg tatagttaca acatccagta aaaaaattaa taaagtcata attatcatac
     6961 ccttcatcaa attcatcaac taccatattt ttatcgccaa agaaattacc tctttgaagt
     7021 agtttagttc ttttcattat aaaaacctcc taattatctt atttcatacg aatatttatt
     7081 taaagttata tctgtctgac caattgaata tgccctatta gattttgttt caacaaagtt
     7141 attatccatt tcatgtatat atctaactct atttgcatta aatggtagca ccacattctt
     7201 aaatgtaaca ctaccaagtt ctccagttat acactttcta tttaaatcta acttaaggcc
     7261 ttctatagct attatgtttt tagcattagt taaggtatca taccaatatc cagcttcatc
     7321 tacatctatt tctgactcta attctaagta tattaagata tctaatactt tttcagataa
     7381 tttattgtaa ctatttttta atacagatag ttcagtttgt atttgacctc ctattacatc
     7441 atcaatagat atatttttaa ttttttctac tacctcattt atagctcctg ataagttttt
     7501 agcttctgta gataagagtt cttcgccaat ttttactttt atttctttta tatttttatc
     7561 aaacttatca tctaaatctt gaatatcctt ttgagttaca aaaaccaacg tcgggtctac
     7621 ttctaatgta acatttgaag tattggacaa ttgtaaaatc atttttacaa ccaaatcttt
     7681 aatactgcca tcttcagcac gtggcttata cgtctctgga tacttaccta tagctattat
     7741 attattctca ttatccagaa cagcagcttc tcttatcata aatccaccaa ctgttcctgg
     7801 taaaatagtc tctataacta tccaattagg atttttttca tcaattctta gagaatttat
     7861 atttccttcc caaactacat tctttaaagc agtttgttcc tctgttggct cataatattc
     7921 tccattacta tctccaactt gaaacttggt taatccaaca agctctccta gtgcagttgc
     7981 atttgctatt ttttctctac caattttggt taatattata taaaattttt cttccaatta
     8041 taccacctcc gatttaggat aaactactac attttctaca cctgtatcat ttgcaatagc
     8101 tatattaatc ttacaattat tttcaatgca tttaatgctt aaaggataaa cctttatttc
     8161 ttctctactt aacattccag ctccacagtt tacaacagca ctacatttta taattggttc
     8221 aaagtatgga tgaactttta cctcttcact acataccata cctccaccac aatagaccat
     8281 acttttatct agtattattg gttctaaata gtgtaataag tgagcaggtt ttactctttc
     8341 aatcatatcg ctacaatcta aaagagtttt atagtcacag ttatttgcta taaaactcaa
     8401 tacgaatgta aattcatcac tataaacttt tatatctgtt tctgattttg tatatgcctc
     8461 acatatactt tttataactt caatagtact agtacctctg cttctcattt tagcttttat
     8521 attgctacgt cttgtgtcaa aatcaagttc ttttttttca atgcagagta tgtcttccca
     8581 catatctaat ccccatgttg cagtgtcaac aaataattgg ttaaaggtat catcaatatc
     8641 ttctttaaga atatttaatt ctgtatcgta tgcaccttgt atctcctcta caatgtaatt
     8701 tctatcaaat gatggtagtt tatcaattag cttcattgat tttccacctc actaaaatta
     8761 acagttgtta cactaggtat tttatcttca ttaatagtta tattatctgt acttccattt
     8821 ataagtagct tacttaaatc gtgtacacca gtagtattta taagtattcc cattactttt
     8881 gtatagatta tttctcctct aatatctcta aggtatgtat ttatactttc taggaaagat
     8941 tcttttacat tgtctaatgt atatccatct tctagtttca ttactgcact tatacttatt
     9001 tctattggta atggtgtcac aactgttata gttggtccaa taggcttctc ttcatctata
     9061 tgttgctgac acctttcaat agtttctgta tcaacagctt gattattttt cccaaatatc
     9121 aagaccttaa ctgttcccgg accatcccat cttggataaa ccttaacatt atagactcca
     9181 tctacttcca aagcccattc ttcatagtga gctttatttc cacttgtagc ttgatttctt
     9241 tggattttat aaaatctttc ttttagctct tcatctgtct ctatatctgt accaccttca
     9301 aaacttcttg tgttagtaat ctttgttact ccagatatat tatcaactag tttaaattca
     9361 caatttgtag gtaaattata tttcttacca acttccagag cttgaactgg gctattgtca
     9421 ccttcttcac taccaatagt tacatctttt attactacaa atagtaaatc tctatatgat
     9481 attactgtgc cattatttat tacagtacct ttctctccaa taaattcaac ttctccattt
     9541 gactctgtac ctaactttct atatacacca aattcattga ctcttttatc aagaaactgg
     9601 ttatatgtgt cttgtataaa agccatttta tgtatctttg aaagttctag atatatcttc
     9661 gaaagttcta gattatttcc agataccatg ttgtttagaa aagaaccttc tcctttataa
     9721 atatcaagat taatattttc aagagttcta ttttttatta cttcatatgt ctggtcacta
     9781 tacatttatt tccacctccc catagatagt ggatactttt acatttgcac tcaatacgtc
     9841 tctgttaaag ttagcacttt ttacgtttac ttctaatata tatggattta ttagaagggc
     9901 ctcttttatg aatctactag cttcactttc tgtaagacct tttgtatatt tttgtcctat
     9961 tagttctgaa agctctgtcc catattctaa agagtatatc tcatgttcat atctatttgt
    10021 ctttatacac ttgtatatcc aaacttttaa ggcttctttt ttttcaatta ttttaaagtc
    10081 acctttttct aaaataggtt catctttttc aaaatcccat gccacttcac gaaagattgg
    10141 caattcttct gttttaggta agatataatc ctctgggaca cctataaaag gaaatattgt
    10201 actcattata agctcaccaa cttacttaca acagcaaatt tttcgcctat tctaaacatt
    10261 attaccatat ccccagattc aaagttatct ataaaaggat tttttacttc atgctggtgt
    10321 tcttgatttg tttctgtgtc aaatgtttct agctgtctat caatcatcca actatctatc
    10381 aaaatatctt ctttttctaa tatgatgtta tttgtctcta tttttaaatc tggtaattta
    10441 cttttaattt ttccaataaa aaaagaaggt tgattatgat actttccttc ttctcttatt
    10501 atccctataa attcatttat tggattagcc attatatcac catcctttta taatactctc
    10561 ctagctgtat taaaatctct tctactactt aacttactta ttttaactac atcacctttt
    10621 tgtggagcat gtaaaaattc tccattacct atatataatc caacatggct gactggatta
    10681 tgaaaaaata ctaaatctcc tgcttgaaga tttttttgtt ctactttctt gcctttttta
    10741 gattgttgat ttgacgttct tggcaaacta acattaactt ttttaaaaca atagaccatt
    10801 aaaccagaac agtcaaaact acttggtcca tttccacccc atttataagg tttacctaga
    10861 tgtttttttg cttctgatat tactatttta gctttatcag atacattacc agaataacca
    10921 ccaataataa tttttccttt tcttcttcca aatttactag cttcttcaac atttccaaat
    10981 aaaatatcta tatggtatgt tccattcttt tctatagtta ttgcaggtcc tacgtcattt
    11041 actttataga ctccatcacg accagaaact cctgtaatta cctgtatttc atctccatag
    11101 tttaataaag gatgtttatt taaaaactct tttgtataat aagtttgctc atatttacca
    11161 accataggag cagcgcaagt ttttttagat gggtcaagtt ttttctttct acaatctgta
    11221 tctccacctt cttcttttct aggacagtaa gctgtaaatt ctgctgtaaa ctcttttcct
    11281 cctgcataat cttctccccc taaattactt tcttccttag gttcatcctg tcctgctgac
    11341 ttttcatcca taagattttg aaaattaagt tcaagctcaa tttgatattc tccattttgc
    11401 caagtatgtt tgtctgtatc tatataaaat agtcctacaa gctttgtata agaatcttta
    11461 acttttactc ctctaccagt tatacaactt acatctccat aacctttaag agaacagctt
    11521 ttttctatcc cattaaactc gctatcaata tctacatctt gattttcttg ttgctgaatt
    11581 actttttgca ttattacatt tacttcctta aaaatttcat tatctatttt ttcgctaatc
    11641 ttgcttccat actggtctac cactattact ttatttttta cattttccat gctctccgaa
    11701 aaggtggtat ttataatatt aaatccctct tcaaacataa catttaatgt aacaattcct
    11761 ttttcaataa cattaaactt atctaaattg gcctctatca tatacttttt ctttgtcttt
    11821 ttacttgctt ctgtatatgc actcattatt gtgtcataac catttactcc tataaacatc
    11881 tttgtatact tgacattggt ctttgctatt gttccaactg aaagcctatt ttcagcaaat
    11941 acttgctttg ctatgtcttc aactaactta tctttaaaat tgtatgacac ttcactttgt
    12001 gtaagtaaaa atcccatatc tttagataca aaactaattt cattactgct tgaatcttta
    12061 gacctattaa ttatcattcc tctaaagagt tctttctcat ctacatagaa acagactgta
    12121 ctagctattg gtatatctat ttgttggaaa tttacatcaa atgatgattg tattattgaa
    12181 aactctagtg tccttgatgg agatttataa tcacctgacc atgatacctt gtctactatg
    12241 tcagttatgt catatatact tccgtttttt atgtgtaccc aaatcttaat aataaaaaat
    12301 cacctgcctt ttcatttaat tctataagca gttatttata acttattatc tataatagtt
    12361 attgttttgg agttactttt ggtatcttta ttcttttata ctcttttaaa tctaatgtaa
    12421 aatatacatc tcgagtccca tctctttctt cataactaaa cccttcaatt atgacttcca
    12481 tatttatatt tgtttccgta attgtaaatc ttaatataaa accttccttc atccattttt
    12541 ctatcttatt tacacaatca tatggtgatg gaaaacctgt atagtcacag aaagtatagt
    12601 ctttatttgg aaaaaaactt gatattgaaa tgcttttaag agctacacca ccaaaagttg
    12661 ctatttcacc tattttgagt atactagaag agtttacagc agcttttcca ttaatactaa
    12721 aggaagatgg aaatactgga aatctaaatc tatcttcagc ttgtctaagc cacatttcca
    12781 ttataagaca ccccctaact gtgccaattg gatacttgca actaattttg atgttatctt
    12841 ttctatatca gcttcttctc taatcactat tgtatcagct aacttagcta tgtttatatt
    12901 tccaccattt tttctgcttc tatattgatt tgcttcttgt ttagttaaaa ccatttcacc
    12961 ttcatggagt ctggtttgat agtcattata aggaacatag ttcaatccaa atgcattatt
    13021 acctttctta cctgaataag ctttaccaga atcaccagcc tttgttccag catatggatt
    13081 atctttgtaa ttttttttac tcttttccac tagatttaaa actgttcctg tgatgcttcc
    13141 attttttatc ttattaatca tagtttgcca ccacttgcat atatctttaa ctattttaca
    13201 tactttatct aatatgttaa aaaaagctcc caatattggc tctataattt tccatccagt
    13261 ttccaacaga ggtcctaagg tctcccatac agttttccaa ataacaccca gtgtctctat
    13321 aaaattgttt atttgaggtg catattcacc taaaaaagta aaaattcttg tgataatagg
    13381 actaacagct tcaactacag gtccgattag ttggaatgct gtagttacta ttgtaccaaa
    13441 tatacttatt aatccttgtg caattggtga tttattgttt atactatcta gtaaattatt
    13501 tactaagttt actactggaa gtaatgaatc tattaatgct gtaaaatcga tattttgtaa
    13561 tagtgtactt cctgcgttaa caattgcttt tggtgcattc cctatcattg ttgctgcatg
    13621 attttcaaaa cgttcttgct tattttcaac catcatttta cgcttttcag cctcattaga
    13681 aattcgatat tgagtagaat cagaaaactc ttttccattt tttacacttc tagattgcat
    13741 ctggtcatat ttgttgccaa taattatctt accattcatt acattgccta ttttaccagc
    13801 cattttttca gataagccag ctaaattgcc taatccactt tctataaaag tttttatttt
    13861 ctttagtcct tgacctaaaa tatcatcaaa aggtttaaca atactcttca agccattgtt
    13921 aatgcttgtt tgagctcctg atattatacc tggtattgta ttactcattt ctttagctaa
    13981 ccccatctgc tttacactta tgccttccaa tgcttcatca aaagttttat aattttctcc
    14041 taccagagta ttaagagatt ccatgtttcc ttttgctgca cttaagaaag ctgctgatac
    14101 atcttgttca ctacttgtat tcatatttaa agctctaaca tctaccatag cttgagttat
    14161 atctttttct tttttgccac cagtaatatt actaatcatt ttagctttag ttcccattgc
    14221 aacaacatct tcagggtcaa aaggcgtatc atttgccatt cttactaaac tgccataata
    14281 atcatcaaca tctatttgat taacttttcc agtatttctt ttcattacac tttctagaac
    14341 aactttctga ttttgttcct gaccaagtaa atcaacaccc tttttcacag cgacacctat
    14401 agtagcacca tttgtcaaat cagatatctt cccttttatc gaaccaagta ttccacttgc
    14461 catatccttt acagctattg caccactcca taccttacct gcaaataatc tcaatttggg
    14521 aaatacttta cttataatgc cacttgccat atcttttata gctattacac cactccaaaa
    14581 agtattttta aacaacatta acttaggcaa aattctaccc atagcactac tcaccatatc
    14641 tttaagatta atacttcctt gataaggacg tttctctact tgttttaatt ttcccaagat
    14701 actacttatc actctcttag tcatatcttt tgcttttatt acgatattcc aaatcttgcc
    14761 accaatcttt tttaaatttc caagtaattt atttttagtt ttttctactt catcactttt
    14821 agctttaaat attaatataa tcataatatt tgaattaaac tcatttttct tttgaatcaa
    14881 cttattaaaa taatcaataa tcatagctat agttattgct agtctcataa tacttccatt
    14941 taaacttcca ataactcctg aaacagcatt tatgctagaa cttaattttg tcatagttga
    15001 acttacacta ctagcagtag tttgaacttt tcttaacgag gaatttaaac tgtttactct
    15061 attacttgca cttccaatag aattttttat gctactagcc attacattag tggaaagtga
    15121 aacagccaca gttgctctcc taaaattatc agttactgat ataatttgat tcatttttgg
    15181 tgtgtaatta tctgtaaggt acatatttat accttcttct ctcacatttc ccattacata
    15241 ccacctcctg tcaaaggaca cactccatat ccatgtttca ttttttcaac ttcttcctca
    15301 atttctaagg cgaaaaaagc ttcaataagc tttaattcgc ctttatccat tgcataaaaa
    15361 agggacggtc ttataccttt tttcttccaa taatagtaca ttattgtagt cctggtatcc
    15421 gtccctatta gttttttacc tcttctatca ctgcattttt accataaccc ataacatctt
    15481 gaagtattct atataagttg gtaatttcac ctggtaataa caattttctt gctaattctt
    15541 ttggtgttgg agccttaaat ttatccatta gctccttatt tttaaataaa agctttccat
    15601 ccaaatcaca aacaccttct ataattgtaa gcatctgcat ctccggtaag tctatatccg
    15661 ttttcatatt ttcatcaatt ttaagacaag tattttgtat ttcttcatct ttacttattg
    15721 ttaaagctct acacattact ttaaattcct gcccaaatat agttgataat ctcttaagtt
    15781 caactatagt acttggtctt tctaagtttt ctgtatctgc atttaaaagt aaatctacta
    15841 tgtttatatt ttttaataat ccattttcac tcattattat ttgctccttt atcaatcaaa
    15901 ttcaatcaac taactttttt acttattaac tatctgaatt tattaagtat atatttacta
    15961 aatatcagta taaaaccaaa tataaaacta aatataaaac taaattatat caagaaaatc
    16021 atactcagta aatgtgaaag gtgcttctat ctctcctttt acgccaacct cccaatcagc
    16081 taaagttaaa tcatcaaatg ctatattctt tactgctatt ctttctgctc cataagaatc
    16141 tgggtcattt aatttagata tagcaacaaa tctaggttca gaacctctct ttatcttttc
    16201 tccaattaac ttgtgcattc ttgaactaac atggtataga gttattgagc cttttccttt
    16261 atatcccata tactttgtat cagtacccat ttgacctgct attataatat cttcttttgt
    16321 aaattccatc tttgcttgaa acttctttac ttctgctact ttgtttccat caagccaaag
    16381 ttctccccaa gtaccactca ttacatttct agcttccata tttgccatat taataatcct
    16441 cctatatttc tattgataaa tctatatctt ccatagcatc aagtactttt atttttgctt
    16501 ttaaaaatac tttagaacct gtgttagctt cttttatttc ttgtaatgtc atataagata
    16561 aatctactcc ttttgatttt aaatacgatt tttgtgcttc aaaatctatt tcaacagtag
    16621 aatcagattc tataagtgct gatttttcta attcttctaa ataacttttt atagctacta
    16681 tcaataaaca tttgttgtca taactgttag taacctttcc tatatagtca tctattatca
    16741 cctttcttat gtcactatgt ataatatcta aagtgtcaac tatttttatt ttctggaaca
    16801 tttctccttt ttctgctgtt aattcagtta aagaatttac tcctctagca attcttatag
    16861 ctcctgcttc tttaataagt ataagctctc ctttattaac ccttgattct gcatcaactt
    16921 tcgtcatctt aggtatatcg actacatcac taagtttagt ataagttact gattgactta
    16981 aaggtgtacc tgctataagt ccagccaccc tacttgtaaa ctcatcaaca ctgtatttct
    17041 tttctccaac taacacatct tctgtagtaa aattaattat cccttcatga tttcctacaa
    17101 cttttcctaa tacagcttta accttaacct tatctatatc tctaagttta attatccaat
    17161 tttttatagc agtcttatca gcttctactg cttttggcat acatagataa ttaaattcct
    17221 tagtctctaa aaaatctaat gcagtttgaa tatctgcttc tccttctatt acataaacta
    17281 ataatttatt tggagtgtta acatttccca tcaaagctaa atttatatat tctttatttt
    17341 cagcagataa atcaactggt atatcctctt tttcatgtat ttcattaaga cctagtgcct
    17401 tagcatcttt aagcaccatt gcaattattc ctctagctga acgttcttta acagttgtag
    17461 ctagctcctt aaatgatatg ttgatacttg gtaatcctat agccatacta tcactctcct
    17521 aaatttaaat tgatttcttc catattttca tatactacat cttcttcata tacactatcc
    17581 agatagttta gtgtaaatgt aaagttcaat acataaattg actcttcttt tttaatactt
    17641 tgttctagct ttgttatatt taattttctg tctgttacct ttatatttct gttgaatagc
    17701 ttatttaatt catctgaaat ttcatacatt ctaatactct ttgactgtgg ttttggtaag
    17761 tattttatag agataacaat atctttatta ttcgtattta acattatagg tgtacaaata
    17821 actgatagta tacttacaaa aaaacaagac ccttcagagc cttgcacact ttcatcatca
    17881 ataaaaatac tttcagtaaa gttttttttg agaatctcaa taattgtttc taatatttcc
    17941 ttgtatttaa gcaaataatc accacactat cactaaatat tcttgcttaa tctgataatc
    18001 ttactataaa tgatataagt catttttaaa tatatatctc atttattcct gtcaagtatc
    18061 ttctatattt ttaggtaata ttattctttt caatctatca cctccttcaa tatggatttc
    18121 tttcacaata acattatctc acatatttta ttgcctattg ttgcaatggt gtatataaag
    18181 tgttgtaaaa ttgtcctatt ttttataata tctcactcaa tttaaatatc atctttttct
    18241 ttaaattgta gcaagatgtt ctatctaagt gtagtttcat agaaatatat gtcatattgt
    18301 tttttgtctt actattataa aaaagattaa aaaattccat ttctatatca tttagacacg
    18361 ttagagcatt ttctatcttc tctttttcta tttctatatc ttctttttcc atttttaatt
    18421 ttcttaatct ttcctctctt tttaacacct cattttccac agaagagctt atgttatatg
    18481 tgttactagt cttttctgta tataccattg ctccacatcc agagtattca ttctcacatc
    18541 tttttatgtc caagttaata ttctttatct ttatatccaa atacttataa ttatgtaatc
    18601 ttttatctgt ttctttaaat aatatatctt ttttgttttc catagtactt ctcctttttt
    18661 atatacttat tattagcttc aaatttacaa taatctcttt agtgcacagt tttttactaa
    18721 tctcacaact ttcaagaaca tattgttcta tgactgtata aaaaaaactt cttctatatt
    18781 ttgttctaaa atactagaaa tcttcttagc cataagaagt ccaggatttt ttatgcctct
    18841 ttctatttga gatatataat catttgtaat tccagccttt ctggcaagtt gaatttgtgt
    18901 catacctttg cattttctat aatatttcaa gttattttta aacattgttt tatacctcca
    18961 ttactattaa gaatattttg ttcttcctca cttagattat atagaattat ttgttcttta
    19021 tcaatatatt ttagaaaaaa atattctttt atattttatt agttttattt tttagaacaa
    19081 tttgttataa tctatacata aggtggtgtt ataatgtttg ccaaaagatt aagagaactt
    19141 agaaaggaat ttggattgac tcaaagagaa cttggagaaa aagtaggcgt ttcccaaaga
    19201 gtcttagggt actatgaaac agaaaacaga tttcctgatg aacacatttt aaataaattg
    19261 gctgatgtat ttaatgtatc agttgattac cttcttggaa gaaccttagt taaggaaaat
    19321 attgatacag tagctgcaca tagaaaaaat ccacatgaag agttacctga agaagcacaa
    19381 gaacaactta atgattatat agaattttta ctaaataaat ataaaaaaaa ataaaccata
    19441 tgatgagcag tctatttttc tgctctttta atataattta aaggcaaaca tacattctat
    19501 tttaggggga tttttatgaa taatttggat aagctttttg aattagcttc tcaagaaggg
    19561 ataataattc attataccac ttatattgca ggtgatttag aaggactata tataaacaaa
    19621 catggtatta aaattatatc attactcagt aatttaaaac aaaactcaaa aaaattaaca
    19681 tccatcttag ctgaagaact tggacatcat ttcactagcc ttggatacta tgtatcctct
    19741 tataacgatt attatacaaa aattattata gataagtgcg aaaataaagc cttaaaatgg
    19801 gcttgtgagt tcttaattac tgaagaagat ataataaaca taattaactc tggcatcacc
    19861 tgcgtttatg aaatggcaga catacttaat gtggatatta ctttctttca aaaaagatta
    19921 gaatttttat cattaaaaaa acagtcttta caacttggaa ataataaata tttaatatta
    19981 actaatttgc cgtatttcta catatttgat ccaatttcat aataaaaata gtagtaa
//
LOCUS       DAMJYC010000072        19805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJYC010000072 DAMJYC010000000
VERSION     DAMJYC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.073"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..191
                     /locus_tag="PC472_002755"
     CDS             <1..191
                     /locus_tag="PC472_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2728071.1"
                     /translation="KVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
     gene            309..614
                     /locus_tag="PC472_002756"
                     /pseudo
     CDS             309..614
                     /locus_tag="PC472_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            627..1532
                     /gene="truB"
                     /locus_tag="PC472_002757"
     CDS             627..1532
                     /gene="truB"
                     /locus_tag="PC472_002757"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2728072.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1663..2592
                     /locus_tag="PC472_002758"
     CDS             1663..2592
                     /locus_tag="PC472_002758"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2728073.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2696..2953
                     /gene="rpsO"
                     /locus_tag="PC472_002759"
     CDS             2696..2953
                     /gene="rpsO"
                     /locus_tag="PC472_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2728074.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3193..4038
                     /locus_tag="PC472_002760"
     CDS             3193..4038
                     /locus_tag="PC472_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2728075.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4163..6265
                     /gene="pnp"
                     /locus_tag="PC472_002761"
     CDS             4163..6265
                     /gene="pnp"
                     /locus_tag="PC472_002761"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2728076.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETGMIP
                     RAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEARPS
                     RGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTLSLL
                     DAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQMDI
                     KIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDKIKD
                     VIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVGKTY
                     KGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEIDEKG
                     RVNLSRKVLLPKPEHKNK"
     gene            6531..7274
                     /locus_tag="PC472_002762"
     CDS             6531..7274
                     /locus_tag="PC472_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2728077.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7432..8679
                     /locus_tag="PC472_002763"
     CDS             7432..8679
                     /locus_tag="PC472_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2728078.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINLIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8714..8959
                     /locus_tag="PC472_002764"
     CDS             8714..8959
                     /locus_tag="PC472_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2728079.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            8969..10192
                     /gene="dapG"
                     /locus_tag="PC472_002765"
     CDS             8969..10192
                     /gene="dapG"
                     /locus_tag="PC472_002765"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2728080.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10305..11039
                     /locus_tag="PC472_002766"
     CDS             10305..11039
                     /locus_tag="PC472_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2728081.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11162..13573
                     /locus_tag="PC472_002767"
     CDS             11162..13573
                     /locus_tag="PC472_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2728082.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13576..14634
                     /gene="mnmH"
                     /locus_tag="PC472_002768"
     CDS             13576..14634
                     /gene="mnmH"
                     /locus_tag="PC472_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2728083.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14628..15962
                     /gene="rimO"
                     /locus_tag="PC472_002769"
     CDS             14628..15962
                     /gene="rimO"
                     /locus_tag="PC472_002769"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2728084.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            15949..16491
                     /gene="pgsA"
                     /locus_tag="PC472_002770"
     CDS             15949..16491
                     /gene="pgsA"
                     /locus_tag="PC472_002770"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2728085.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16779..17825
                     /gene="recA"
                     /locus_tag="PC472_002771"
     CDS             16779..17825
                     /gene="recA"
                     /locus_tag="PC472_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2728086.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18001..19542
                     /gene="rny"
                     /locus_tag="PC472_002772"
     CDS             18001..19542
                     /gene="rny"
                     /locus_tag="PC472_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2728087.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8097 a         2007 c         3840 g         5861 t
ORIGIN      
        1 taaaggtagg taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg
       61 aggaagttaa ggatttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg
      121 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc
      181 aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
      241 aagacatatt ttaatatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg
      301 aggttcagtc aaaggtatct agtatagttt taactcaaga catgatgaat agaaataata
      361 ttagctttaa tgatgttgat ggaataacaa catatacaag agacataaaa ggtatagaag
      421 ttggaatttt atttaaagag aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa
      481 attatgttga tgtaagtgaa atagccaagt tatttggcgg tggtggtcat gtaagggctg
      541 ctggatgtac tattagagat agtattgata atgcaaagaa aatggtacta gaggcagtgt
      601 taaaatcaat ttaaggtgac agtaaaatga ataagattat aagtatattg aaacccacag
      661 gtatgacatc tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagttg
      721 gtcatacagg tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag
      781 ctacaaaagt atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttactt
      841 taggtataag tacagatact tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt
      901 ttaaatttag taatgaagac atagaaagag cttttgacac acaaagagga gagataaatc
      961 aacttcctcc aatttattca gctttaaagg taaatggaaa gagaatgtgt gatttagtaa
     1021 gaagtggtag acaatctgaa attactttaa aaacaagaag agtaaatata aaagacataa
     1081 aaatactaag tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt
     1141 atgtgagaag tatttgccat gatataggag aatatctagg ttgtggagct catatgtcgt
     1201 ttttgaatag aacatcttct ggtaaatttg acttagataa ttcaataaca ttagaagaat
     1261 tagaattatt ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtcc
     1321 ttgattcgtt taattatgtt gtattaaacc ctaatgctat aaaatattat agtaatggtg
     1381 gctcaataga tgataaaagg tttttaaaaa ataatttcga taaagatgat gaatttgtaa
     1441 gagtatacag tacagataat ttcttaggat taggaaagtt atcaaaacac aataatacaa
     1501 taagtgttaa aagtgataag atgttcatat aaccaatgta tttaaaaaaa caacaaaaga
     1561 gatagtacta taataacttc tatagtctat ctcttttact tatgctataa tagtttgtga
     1621 ataagttaag agtaaattat attttagcgg ggaacattta ttatggtgat tataaaatct
     1681 ataaaagaaa tagcaaacat aaaagaaagt gtcattacca taggaaattt tgatggtgta
     1741 cacaaaggac atcaagttct tataggaaaa acagttgaac atgcaaaaaa agaaaatata
     1801 cagagtattg tatttacatt tgcaaatcat cctgtaaatt actttagacc aaactcaaca
     1861 aaaaatatta taagcaatga tgataaaata aaattattag aagatttggg aatagatata
     1921 gttgtaatca taccatttga tgagtatatg actaaaatac ctgcaaagga tttcgtagat
     1981 gagatactag taaaaaaatt aaaagctaaa aagatagtaa ttggacatga ctttacattt
     2041 gcaagggcaa aagaaggcaa tgttaatcta ttaaaaagct tagaacatga atttggtttt
     2101 gaggtagaag taataaaacc catcaaaatc aatgatgtaa gagtgagtag tacttatatt
     2161 agaagtttag tatcccaggg tgatatggct aatgtcaagg aatatttggg tagaaattac
     2221 aagttagaag gttgtgttat acattctaaa caccttggaa gaactatagg attcccaacg
     2281 gcaaatattg accttaagaa taatatgctc gttccaaaga gaggtattta tgcttctatt
     2341 gtacatattg gagatgagac ttattttggt gctacaaatg taggctataa cccaacagtt
     2401 aatggaaaaa ctttgtctat tgaaacaaat attttagagt ttgatagaga tatttatggg
     2461 gaaaatataa ttgttgaatt tttggaaaga ataagagatg aaagaaagtt taattcaata
     2521 gatgacttaa aaaatcaatt gtataatgat acaaattatg tgtatgaaaa ctatgtttgc
     2581 aaaaacaagt aatgtatgat aaaatgatta aggaaaataa ccattactcg gcatttgaat
     2641 actccaatca atgcttagta ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat
     2701 aaacaaatca gaaataataa aagaatatgg aagagctgaa ggggatactg gttctccaga
     2761 agtacaaata gctttattaa cagctagaat aaacgagtta aatggacatt taaaaactca
     2821 taagaaagac caccattcaa gaagaggact attaaaaatg gttggtagaa gaagaaactt
     2881 attagcttac ttaaaggaaa aagatcttga aggatataga gcattaatag ctaaattagg
     2941 attaagaaag taatttcatt ctttataaaa aagcaggtat agtgtatacc tgctttttta
     3001 tagttaattc attataaagt aaaatagtta ataataaaag tagtttatat gttataaaaa
     3061 attataaaat gaataatatt gggtaagaat attaaaatag tatacaaata cataatcaat
     3121 ttaaaaggaa agacaggata aatatagaaa ttttataaca ggataattag ttggaataag
     3181 gaggatatta ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat tccagatgaa
     3241 atatcggaaa aagattatat agagatagtt ccacttacag ttatttttaa tgatagggaa
     3301 tatatagaag gagtagatat aaagaaagaa gagttttata aaaaagtaaa agaaatcaag
     3361 caaataccta aaacttcaca agctacatat atggagttta aagaagtgtt tgataagttc
     3421 ataacagaag gttatcatat aatctgtatg acaggggctg caaacgcatc aggaactttt
     3481 caaagtgcta tgatagctaa aaatgatgta aatgaaaatg aaaaaataca tatatttgat
     3541 actagaaatt tatctctagg tagtggacaa tatgttataa aggcatgtga acttcttgaa
     3601 gaaggactgg gatttgaaga aattatagat gaattagaaa atactcgcag tagtgtaaga
     3661 ttgctttttg caccatatac acttgatttt ttaaagcaga gtggaagagt tccagttgca
     3721 actgctttga taggaaatat gttaaatata aagcctatat ttttctttga taatggggaa
     3781 gctaagttgg taaataaggt tagaggaata aagaatatag caagtaaatt ggtcgatata
     3841 attttagaaa tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat
     3901 aatttacacg attgtgaaat attaaaagat gaagtaaata aaagaataaa agcaagaaga
     3961 gtattattta ctcgaggagg agtttctatt tgctctcata cagggccaga tatattggct
     4021 ataagttgtt ctaattagta gtaatataag gtttttgcat atatatgtga agataatttt
     4081 atattgttaa tttatagtat ttttgataat atagtaaata gagtgatata cgtgtttttt
     4141 aataagagga ggacaagcat taatgtttga acataaaata tttaaaatgg attttgcagg
     4201 aagagaactt tctgtagaaa taggaaagat ttgtgaaatg gctagtggta gttgtatagt
     4261 tcgatacagt gatagtatgg taatggtaaa tacaactaaa tcagccaaac ctagagacgg
     4321 aatagacttt tttcctttaa gtgtggatta tgaagaaaaa ttatactctg taggtaagat
     4381 acctggaggt tttttaaaga gagaaggcaa gccttcagag aaagctatac ttacatcaag
     4441 attgatagat agaccaataa gacctttatt cccaaaagga tttagaaatg atgttcaggt
     4501 agttgcaaca gttctatcag tagaccaaga ttgtacacca gatatagtag caatgatagg
     4561 ttcttcaata gccctttcta tatcagacat accatttaat ggaccaacag gttcagtatg
     4621 tgttggcctt gtagatggtg catttgttgt aaatccaaac gcagagcaaa gagaaaaaag
     4681 ttcaatgcat ctagttgttt caggtacaaa ggaagcaata atgatggttg aagccggagc
     4741 tgatgaagtt ccagacgagg taatgcttga tgctatttta tttgctcacc aagaaataaa
     4801 aaaaatagtt gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt
     4861 tgaattatac catgcaggag aagaaataac tcaattggtt cgcgaatttg caactgataa
     4921 gatgaaaaaa gcagttcaga cttttgaaaa gttagagaga atggaaaata tggatagagt
     4981 taaagaagaa actttagctc actttgaaga aacttttgaa gattttgttg gagatataga
     5041 agaagtatta caagatataa taaaagaaga agtaagaaaa cttatagtac atgaaaatgt
     5101 aagaccagat aatagaaaac ttgaagaaat aagacctata tggtgtgaaa ctggcatgat
     5161 acctagagct catggttcag ccatatttac tagaggacaa actcaagtac ttaatgttgc
     5221 tactttaggt gctttaggag atgttcaaaa actagatggg ttagatgaag aagaaaacaa
     5281 gagatatatg catcattata atttccctgc atatagtgtt ggagaagcta gaccatcaag
     5341 aggaccagga agaagagaaa taggacatgg tgctttagca gaaagagctt tattaccagt
     5401 aataccatct caagaagaat ttccttatgc tattagatta gtttctgaag tattaagttc
     5461 aaatggttct acatctcaag caagtgtatg tggctcaaca ttatctttac ttgatgcagg
     5521 tgtacctata aaagatatgg ttgcaggtat agcaatggga cttataaagc atgatggtaa
     5581 ggttgcagtg ctttcagata tacaaggtat ggaagaccat ttaggcgata tggactttaa
     5641 agtagctgga acagagtatg gtataacagc aatacaaatg gatataaaaa ttgatggtat
     5701 agataaagaa attctacaac gtgctttaaa acaagctaaa gagggtagaa tccatatact
     5761 aggagagatg agaaaaacta tatctcagcc aaaaccagaa ctttcaccat atgcacctaa
     5821 gattgttaaa atgcaaataa atcctgacaa gataaaagat gtaataggac ctggtggaaa
     5881 gataataact aagataatag acgaaactgg agttaagata gatattgaac aaactgggga
     5941 agtatttata tcaggtattg agattgatat gattaagaaa gctcaagaac ttataaataa
     6001 catagtagtt gaaccagaag taggaaaaac ttataaaggt aaggtaagta gaataatgaa
     6061 ctttggagca tttgttgaga tacttccagg aaaagaagga ttacttcata tatctcatat
     6121 agctcatgaa agagttgcca aagttgaaga tgtattgaat ataggggatg aagttgaagt
     6181 taaagttaca gagattgatg aaaaaggaag agttaattta tcaagaaaag ttcttttacc
     6241 aaaaccagaa cataaaaata aataacttat tatataatta aaagcaaagt cttaggattt
     6301 tgctttttta aatacaaaaa agtagataat tttaaaatga tagttatgga tggcttatag
     6361 ttctttttct gtcaggcttt actcttataa agaagctatt taaaagctga ttaatagaga
     6421 gctttattga tatatataga atcacttaag ttatatttag tgcataaaca gagcttattt
     6481 ttaatagtat taaataagaa acaatttgta cataattagg gggagtttta atgataatga
     6541 tggtacttaa taagagtaag ctcaaaaaaa tcataatact aattttagta gtgttattag
     6601 tattatcagg aggggtaatg gtattaaagt tatttaacaa caagccagta tttaatttat
     6661 ttaatggtgt agatttacca tatgaaagag gtacaaagga taaaagtgga tatgttgcat
     6721 taacctgtaa tatagacctt ggatgggaaa cagagtacgt agaatccatt ttagaaacac
     6781 ttaaaaaaga aaatgtaaag attacattta atgtaactgg aaaatgggca gaaaagaata
     6841 aggatgaatt attaaagata aaaaaacaag gacatgaaat aggtaatcat ggatataaac
     6901 atttagatta ttctacctta tcatatgaag acaattatga gcaaatagag acctctaaaa
     6961 agataattga agaaattata ggtgaaaaaa ctaaattttt tcaagcacca gcaggttctt
     7021 ttggtccaga aacagtaaag gctgctaaag cattagggta tacttctatc aaatgggatg
     7081 ccgatactat agattggaaa tacaaagacc aaccagaggt aatcattgat agaatgaaga
     7141 aaaaagacat taaagatagt agtataatct taatgcatcc aaccaacgca acgacaaaat
     7201 gtatagatga tattatagct attgtaagag aaaaaggact aaaaccagga aaacttagtg
     7261 atgtatttaa gtagacaaag tataaatatt tggttaagat tgtataagtt gtagttgaat
     7321 taattaaaag actgacttat aatgtaatac atataaaaaa taaatataat taaaacatag
     7381 tgttttcaat aatccaatat gaaacaaaga ataaattgtt aggagaatgt aatgtataag
     7441 acaaaaatac tggaaaatgg gcttacaata attggtgaag aaataccata tctaaagtca
     7501 ataacattag gtatttggat aaatgcaggc tctaggatag aagaagcaca ggtgagtgga
     7561 acttctcatt ttatagagca tatgatgttt aaaggaacca aaaacagaac atcaaaagag
     7621 attgctagtt caatagataa tttgggtggt caaataaatg catttacaag taaagaatgt
     7681 acatgttatt atgtaaaact aatagatgag catatagata caggaattga tgtattgagt
     7741 gatatgatac taaattctaa atttgataaa aatgatatag ataaggaaag acttataata
     7801 ttggaagaac ttaagatgta tgaagactct ccagatgact tatcttatga tttattagtg
     7861 gaaaatatat atgctaatga tggattagga atgaatataa ttgggacaaa agaatctcta
     7921 tacaatataa ctagagaatc catgttagaa tacttaaata agtattatat tccaaataac
     7981 gctgttattt ctatagctgg aaactttaac tttgatgata tggtggagaa aatcaaatca
     8041 aaatttggac actgggaaaa gaaaaattta agtatagaca taagtgaggc taagtttaat
     8101 ccttgcttta tatcaaaaaa taaagatact gaacaggtga acttagctat gtgcctaaaa
     8161 ggtattcctt ttgaaaatga tgaagaagtc tattcaatgg cagtggtaaa taatattttc
     8221 ggtggaagta taagctcaag gctttttcag aaaataaggg aagaaaaagg tttagtatat
     8281 tcaatatact catcacagac attatatagg aaatgtggtg aattaggtat atttgcaagc
     8341 atgagtacag aaaatttaca agatgtatat aatttaataa agaaagaaat tgaaaatata
     8401 agagaaaact atcttacaga aaaagaaata tctgaaagca aggaacagtt aaaaggaaat
     8461 tatatattgg atttagaaag tactagcagt agaatgatgt ctactggtaa atctatgtta
     8521 cttagtaaaa aagtaaaaac tacagatgaa atacttgaat gtataaataa tgttaatatt
     8581 aatttaataa agaaggttgt tgataaggta ttcaatatcg aaaatattgg tacatgtata
     8641 gttggaagag atgtagaaaa gattctacac ttggattaat attgaaaaaa gtaaatctct
     8701 ggaggtataa aatatgaatt tatctgaaat agcaggtaag gaaatagtta acttggtcac
     8761 aggagaaaga ttgggggtca ttggagaatg tgaccttata ctagatgaaa ctacaggcgg
     8821 tatattagcg cttctaattc caaaagaaaa aggatttttt gccagaagaa aagataagtc
     8881 atttttagaa gtaccatgga gaaatgtaaa aaaaataggt aatgacatga taattataga
     8941 atatgaggga tatatttagg aggaaaaaat gagtatttta gtgcaaaagt ttggtgggac
     9001 atcagtagaa tcatatgaaa aaatgaatga agtatgtaag atagtaaaag catacaaaaa
     9061 aaatgatgaa gaattacaat tagtattagt agtttcagca atgggaagaa aaggagctcc
     9121 atatgctaca gatacactta taaatttgtg tagtgcagta aatgatgagc caagtaaaag
     9181 agaacttgat ttaataatgt cttgtggaga aataatatca ggaacaattc ttgctaattt
     9241 attgaatgca caggggatag attctgtttt ctttactggt cagcaagcag gtataattac
     9301 aagtgatgaa tattctaatg caaagataaa gtatataaat cctaaaaagg taaaaagagc
     9361 tttagatgat ggtaaggttg tagttatagc tgggttccag ggtgtaacag atgatggaga
     9421 aataactact cttggaagag gaggaagcga tacatcagca gttgctattg gtaaagcttt
     9481 agaatgtgaa acagttgaaa tttacactga tgtggatggt ataatgactg cagaccctag
     9541 agtagaacca aacgctaaag tattgagttt tatagactat gaggaagtat tccaaatggc
     9601 tgacaaaggt gcaaaagtaa tacatccaag agcagtacag ttggctaaaa gtggaaatat
     9661 aactttagcc ataaagaata caatgaatcc aacttttgaa ggtacaaaaa taggttcttt
     9721 atgtagacac ttagaagaca atatagaata tgaacaagaa agagatttta aggttgctgt
     9781 tgcaaataaa gatagcgtag cacaagttaa gattaagtca gcagaagaag tgtttacaga
     9841 agtattaaat gagatagaaa agaaacttat aactatagat atgataaatt tctttatatc
     9901 agaaaaagca tttgttgtag aagatgctga tataaaatca ttaaaggaaa tactagataa
     9961 atttgaatta gattatgaag taaatagaga ctgtgctaag gtaacattga tatgttcaag
    10021 aattgctgat gaaatgtctg gtatcatgtc aaaggtagtt agaggattat caaaagcagg
    10081 ggtatccctt ttacaaacat cagactcaaa tatgactata tcttgtttag ttagtgaaga
    10141 agatatgcat acagcagtac atgctataca ccaacaattt tatttaaaat aaactgtata
    10201 atacaattat tttttaatca aatttaagaa tttcatatat atcttaatat atttgattta
    10261 tttggtaaaa ctaaattaaa tataattttg ataggagttt ttttatgaaa gattatagag
    10321 aagataatga aaaatttgat aaagataatt gtagtgaaga atctagttgc gaagagagag
    10381 aatgtataaa gcaatttggt accaatgaaa tgccacctca acctcctaaa gatattcaat
    10441 gtataactat aattggagaa attgagggac attttatagg aaatcctcaa aaaaaagcta
    10501 caaagtacga gcatataatt ccaatgctat actcaattga ggaaagcaat gatgttaaag
    10561 gagtgttagt tgttttaaac actgttggag gagatattga agcaggttta gctattgcag
    10621 aacttttaaa cagtacatct aaaaaagtag ttactcttgt tctaggagga agtcatagta
    10681 taggtgttcc tcttgctaca gcaggagatt actcatttat agcaccaaca gcaaccatga
    10741 ttatacatcc agtgcgaact actggtcttg tgattggtat aaatgagact tttgagtatt
    10801 ttaaaaagat gcaagataga ataattcaat ttataataag aacatcaaat atcaaaaaag
    10861 atgtacttga aaaacttatg catgaaaaag atgaacttgt aagtgatgtt ggtagtgtat
    10921 taataggaaa agaagctgtg gattatggtc ttatagatga agttggagga ctaaaagaag
    10981 cacttaaaaa attaagagag cttataaaag aatctgagga agaaaaaaat aatgattaaa
    11041 atttgtttaa ataataaaat ttatgatata atgatgtaat aaagtaggag tgttagtgtt
    11101 aaggcattag cactttttgc tgtgaagggg taaaacttgc taaaaataag gagtgatatt
    11161 aatggccacg aaaaagaaga aaaaaaagaa ggtaaatacc ttaagtttta atgcagaata
    11221 tcataattta ataacaatat ttataggtgt atttttatta tatagtttaa attctaattc
    11281 tatgggatgg atacctgtgt taatgcaaaa tctttttaag ggactatttg gagggctttc
    11341 aatagcaata ccatttatag taattataac aggactttta ggattttttg atggaaatga
    11401 atatatatat aggctaagaa agactaaact atactacata attattttat ttatatttgt
    11461 attttatgga ttattaaatg caggaactct tcctgtagat agcccactaa aaggtaacat
    11521 gtttgatgat gttatgaaat tgggtgtaag tggacaaggt agtggtttga tagcaacaac
    11581 tattgcgtat tatatgagta aaatatttgg tatagcaggt ggatggttaa taagtatctt
    11641 cgcacttata ctttctgtaa tgtttatatt taatatttct ataaaagatt tggtgtcaaa
    11701 tgcaaaatca aaatctaaag catctaaaga cagcaattta acgtttaaag ataaaattgc
    11761 aaatatgaaa aaatctgcaa tagatatgat gactgatgaa gtagatgata ctactataaa
    11821 taaaaaacca ggtttcttta aagggttaat gtcaaaaggt cttaataatg atgatgatga
    11881 agatgaatat tttgaagaag aaaatagtga tggtgtagat gataaaacta taaaaatagt
    11941 tggatttaat aaggcagaag atgagtattt agaaatatta gaaggaactc aaagtatgcc
    12001 agagttagat gttttaaaag aattacaaaa agctaccaat gaaaatccag ttgtagatac
    12061 taaaccagaa aaaaaagttg atatagctaa acctaattta aatatagaaa aaactcaacc
    12121 tatgagtatt gtagcagagc cagttaatga ggattattca aactataaaa agcctagcat
    12181 tgagctttta aataaagtaa ataagaagtc tgatgaaaat ggtaaaaaga aagtgttgaa
    12241 aaatgcttct cttttagaaa agacactatc agattttgga gtggaagcaa aaattaatca
    12301 agttacagtt ggacctacta taacaagata tgaaatacaa ccaagtccag gagttaaggt
    12361 aagtaaaatt gtaaatttaa ctgatgatat agctttaagt ttagcagcta aaagtataag
    12421 aattgaagcc ccaattcctg gtaaaagtgc aattggaata gaagttccaa atgaagaagc
    12481 tcaaatggtt ggtgtgagag aagttctaga aagtgaagaa tttaataatt ttaactctcc
    12541 tcttgcaatg ggacttggaa aagacgtagc aggaaaaatt ataattggag atataggtaa
    12601 gatgccacac ttactaatag ctggttctac aggttcagga aagagtgttt gtgtaaatac
    12661 attaataagt agtattttat ataaggcaaa tcctgatgaa gtaaaattac tattaataga
    12721 cccaaaagta gttgaacttg caaattataa tggaatacca catctattga taccagtagt
    12781 tactgaccct aaaaaagctg caaatgcttt aaattgggca gttacagaga tgaatagaag
    12841 gtacaaatta tttgcagatg ctcaagtaaa agatgtaaca agttataatg agaaagcgga
    12901 agaaaaactt cctaaaatag taataataat agatgagtta gcagatttaa tgatggcaag
    12961 tgcaaatgat gtagaagatt atatttgtag attagctcaa atggcaagag ctgctggaat
    13021 gcacttgatt gtagcaactc aaagaccatc tgttgacgtt ataacagggg taataaaggc
    13081 taatatacca tctagaatag catttgcagt atcatctcaa actgattcaa gaacaatcct
    13141 tgatatgggt ggtgcagaga agcttctagg taaaggtgat atgttattct atccacttgg
    13201 tgcagcaaaa ccagttagac ttcaaggggc attcatatca gagagtgagt cggaaaaagt
    13261 aatcgatttt gtaaaaagtc aggttaaaga tggaataaaa tatgaagaag atataattga
    13321 aactatatct aaggttaata cttcaaaggg tagtgatgag gatgagtttt tgagtgaagc
    13381 gatagaattt gtcgtggaaa gtggtcaagc atctgcctct atgctacaga gaagatttaa
    13441 aataggattc aatagagcag ctagacttat agattctatg gaagaaagag gaataattgg
    13501 agcaagtgag ggaagtaaac cgagaaaggt attaataagc aaacaggatt tgcagaattt
    13561 agaaggtgaa tagaattgtc agttataaaa atagaaaaag cacttaagct agataaagta
    13621 atatttgtag atgtgagaac tgaaggagaa tatgaagaag accatatatt gaatgcaatc
    13681 aatatgcctc tttttaaaaa taatgaacat aatgaagttg gaacaattta taagatgcag
    13741 ggaaaacatg aagcaataca aaaagggttt gactatgtat cgtataaatt aaaggatata
    13801 tatttacaag ctgcagaact tgcattaaat tatgataata ttgtcatata ttgtgcaaga
    13861 ggcggaatga gaagtggcag tatagtaaat ttattatcat cattaggtgt aaatgtatat
    13921 caacttgaag gtggatataa ggcttataga aattttgttt tagagtattt aaggcatgta
    13981 atggatacaa aaaactttgt atctgttcat ggattgactg gtacaggaaa gactgatttg
    14041 ttacatttat tagatgaaaa aggaatagac gtactggatt tagaaggcat agctaaaaac
    14101 agtggttcta cctttggttt tataactttt gataaaaagc caccatctca aaagaacttt
    14161 gaaacaaaac tatttgaaag tatatttttt tcgaaagaga attatatatt tgtcgagagt
    14221 gaaagcaaac gagttggaag tgttacagtt ccaaatgaaa tttttgaggc aatgacacga
    14281 gaaggacatc atatattatt agaatgtgat attgaaaata gagttgatag actttgtaga
    14341 gattatattt atgataaaga tgaaggtaat atattgattt taaaagaatg tataaacaag
    14401 tttagaaaaa gattgggtaa taaagtagta gatgattata ttgatttatt agaatcaggt
    14461 gaatataaag aacttgtaaa aagatatctt ttagaatact acgacccact ttatatgcat
    14521 tcagtagaga aatataagta tagtaagatt ataaatttta atgatacaca aaaagcttta
    14581 gaagatgtta tagacttata tgagagtata ttggaaggag aaagtgaatg ttaaaaatag
    14641 cattggaatc attaggatgc tcaaaaaatt tagtagatgc agaaataatg atgggaattt
    14701 taaataacaa aggctataaa ttaattggag attttgaaga agctgatgta attatagtaa
    14761 atacatgtgg gtttatagag tcagctaaac aagaatctat agatacgata ataaactttg
    14821 cagagttaaa aaaaactggc aatttaaagt tgttgatagt aacagggtgt ctagcccaaa
    14881 gatattctga agaattaaaa actgaaattc ctgaaataga tgctatagta ggaacaggaa
    14941 gctatcaaaa tatagataaa atacttaaag aattaagtga aatgcatcaa attgtaagcc
    15001 ttaatgatat agagtttgta tttaatgaag atttaccaag atatatatct acacctagct
    15061 atatggccta tttaaaaatt ggagaaggat gttccaataa ttgtacttat tgtataatac
    15121 caaaactaag aggtaaatat agaagtagaa aatttgaaga tataattaaa gaagctaaga
    15181 aattagctga atctggagta aaagaacttg tggttatagc acaggatacg acaaagtatg
    15241 gttttgattt atatggaaaa gaaagattat cagaactttt agaagaactt gcaaaaatag
    15301 atggatttaa gtggataagg gttatgtatt catatccaga atctataaca gaagaattga
    15361 tacaagttat aaagaaatat gataatatat gtagttactt tgatatgcca atacagcatg
    15421 caagtaacaa tattttaaaa ttaatgaata gaaaaactac taaagaagat atattaaata
    15481 aaatcaatct gataagaagt aatataccag atgctatact tagaactaca ataatagtag
    15541 ggtttcctgg tgaaacagaa gatgacttca aacaacttgt ggactttgtt gaagaggtta
    15601 aatttgatag acttggtgca tttgcatatt caagagaaga agatacacca gcagatagat
    15661 tgccaaacca tatagatgaa gaagtaaaaa tacaaagaag agatacttta atgatgattc
    15721 aacaaaaaat atctgaagaa cttaatgaca aaaagatagg aaaaacttat gaggtgttaa
    15781 tagaagaaca gatagaagat aatgtatata caggaagaac acaaggtgat gcagaagaaa
    15841 ttgacagtat agtgtatgta aaaagtgtag acaatcttga agtaggagaa tttgtaagtg
    15901 ttcaaataaa tgatgcaatg gaatacgatt taatgggaga tgttttatat gaacttgcct
    15961 aacaagttaa ctttgtttag aatattttta ataccagtat ttgttttaat tatgttgctc
    16021 aatataccaa ataaattttt aatagcttgt attattttta taatagcttc aattactgat
    16081 gctttagatg gaaaaatagc tagaaaatac aatctagtca ctgactttgg aaaatttatg
    16141 gacccattag cagataagtt attggttatt tcagcactta catgtatgat agaagaccat
    16201 cttgtaagta gttggatggt aattataata gtggctagag agcttacagt tagcatatta
    16261 agagctatag ctgctgctga tggaaaagta attgctgctg gaaacagtgg aaaacttaaa
    16321 acgataactc aaatggtatc tatagtgttt ttattgttag gtgctcagtt tgaaaatgta
    16381 ttgatattaa atattggtga aattctcatt cttatagcta cactacttac attatattct
    16441 ggatgggaat atctatataa aaataaagag ctttttatgt cttctaagta aaaaattatg
    16501 ctttaataaa taaaaactaa tgattaataa ataaaaaata acaacttaaa attttaaaaa
    16561 tgtaaaaaag ggtatactat tatagtatac ctcttttcat tttaagaata ttttcttttg
    16621 tgtatataat tattaataat ggtttataaa taaaatgtaa tgaataaaaa tataatcatt
    16681 agtagcgttt ttaaattgac taggtttgat ttatgttata taattaatat ataacaagag
    16741 aacaaatgtt tgtagataaa tgaagggatg gtatagagat gagtgtagat caagaaaaat
    16801 taaaagcgtt gaatgaagct ttaggtaaaa ttgaaaaaga ttttggtaaa ggttcagtaa
    16861 tgaaattggg agaagcaaca tctatgtcta tagatgttat atcaacagga gcgattggtt
    16921 tagacatagc tattggtata ggaggtctac ctagagggag aatagttgaa gtatatggtc
    16981 cagaatcttc tggtaagact actgttgcgc ttagttgtgt agcatcagct caaaaagatg
    17041 gaggaatagc tgcatttata gatgcagaac atgcacttga cccagtatat gcaaaagctt
    17101 tgggtgtgga tgttgataac ctaataatat ctcaaccaga tacaggtgaa caggctttag
    17161 agatagcaga ggcattgata agaagtggag cgatagatat aatagtaata gactcagtag
    17221 cagcattagt tccaaaggct gaaatagatg gagatatggg tgattctcat gtaggtttac
    17281 aagctagact tatgtcacaa gcacttagaa agttaactgg ttcaattaaa aaatcaaatt
    17341 gtgttgctat atttataaac cagttaagag agaaagtagg aataatgttt ggaaacccag
    17401 aaactactac tggaggacgt gcactaaaat tctattcatc agttagattg gatgttagaa
    17461 aaatagatac aataaaacaa ggtgataaag ttataggtag tagaactaga gttaaagttg
    17521 ttaaaaacaa agtagcacca ccatttaagc aagctgaatt tgatataatg tatggagaag
    17581 ggatttcaaa aattggagac cttttagata tagctgctga tgtagatata gttaaaaaat
    17641 caggttcatg gtatagttac aatgatacta aacttggaca aggaagagaa aatgttaaaa
    17701 aattcttgga agataattta gatttaacta ctgaaataga tgagaaagtt agagcatttt
    17761 acaatttaaa tgaagaacat gaagaatcag gtacttcagt atcaaaagaa attgtagaag
    17821 aataagtaaa aatacaaaaa aataactagt aattaaagac atgccacgct ccctattagc
    17881 ttgacacaat gttgaaaaaa ttataaaatt aagtgaggat aaaatatcta aatttaattt
    17941 taattattag gaatattttt taatcatagt gatgacattt tgggattatt aacgaaggag
    18001 gtggatgtca tagatagtgt agtaatgatt gtaataggtg cagctgttgg tattatagct
    18061 ggatattttg ttagaaaaaa tatatctgaa gcaaaaattg gacaagctga aaacttggcc
    18121 aaagaaataa tagataaagc acaccacgac tcagaaacag tacaaaaaga aaaattacta
    18181 gaagctaaag aagagatcca taagtggaga acagaagctg aaagagaaaa cagagaaaga
    18241 agaacagaag tacaaaagta tgaaaaaaga gtagtacaaa aagaagaagt cttagataga
    18301 aaattacaaa atttagaatc taaagaagta aacctaagtg aaaaattaaa ggtagtggaa
    18361 aagaaagaag aagaagttga agtaataaaa actcaacagt tagaaaaatt agaaagtata
    18421 tcaggaatta cttctgataa ggccaaagaa atcatcttaa ccaatgcgga gagagatgta
    18481 agacgtgaaa tgtctataat gataaaagag atagaaagtc aagctaaaga agaagctgat
    18541 aagaaatcaa gagaaattat aggatatgca attcaaaaat gtgcagcaga tcacgtagcc
    18601 gaaactactg taacagtagt aagcttgcct aatgatgaaa tgaaaggtag aataataggt
    18661 agggaaggta gaaatataag gactttagaa accttaacag gtatagacct tataatagat
    18721 gacactccag aagccgtaat tttatcagga tttgatccaa taagaagaga aattgcaaga
    18781 attgctctag aaaaattaat agcagatgga agaatacatc cagctagaat tgaagaaatg
    18841 gttgaaaagg cgagaaaaga agttgacaat attataaaag aatatggtga acaagctgca
    18901 tttgaaacta gtgtacatgg gattcatccg gaattaataa gattattagg tagacttaac
    18961 tatagaacta gttatggaca aaatgtactt aaacattcta tagaagttgc tcatatagct
    19021 ggtataatgg ctgctgaaat aggagcagat attagattgg ctaagagggc aggtttatta
    19081 catgatatag gtaaggctgt agaccatgaa atggaaggta cacatgttga aataggtatg
    19141 gatttactta gacgttataa agagtctaaa gaagttatac atgccatgag tactcatcat
    19201 ggagattacg agcctcaaac agtagaggct gtattagtta cagcagcaga tgcaatatca
    19261 gctgctagac ctggtgcgag aagagaaact ttagaagcat atataaagag acttgaaaaa
    19321 ttagaagaaa tagctaattc atatgaaggt gtagaaaaat catttgctat acaagctggt
    19381 agagaaataa gaataatggt aaaaccagaa gctataaatg atgaagaaat acatctattg
    19441 gctagagata tgactaagaa aatagaggat gaacttgaat atcctggaca aataaaagtg
    19501 agcataataa gagaaacaag agctatagaa tatgcgaagt aaaagaaata atatctaaat
    19561 aataagaaaa tatttttaga aatttaaatt tgccacaaga tatgataata tatcttgtgg
    19621 cttttaaatt gtttaagttt aattttatat aatgcaaaaa taatctaagt gctaaaagtg
    19681 ctacttacaa aagattaata aaataaagct ttatatatgt attatttata taattatcca
    19741 agtttgtttg atataatgaa gttgtaaaat caatacttta ctacaaaata aatagtaaat
    19801 actac
//
LOCUS       DAMJYC010000073        19517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJYC010000073 DAMJYC010000000
VERSION     DAMJYC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.074"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(141..1169)
                     /locus_tag="PC472_002773"
     CDS             complement(141..1169)
                     /locus_tag="PC472_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2728088.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1217..2077)
                     /locus_tag="PC472_002774"
     CDS             complement(1217..2077)
                     /locus_tag="PC472_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2728089.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2346..2555
                     /locus_tag="PC472_002775"
     CDS             2346..2555
                     /locus_tag="PC472_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2728090.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2555..2821
                     /locus_tag="PC472_002776"
     CDS             2555..2821
                     /locus_tag="PC472_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2728091.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2850..3422
                     /locus_tag="PC472_002777"
     CDS             2850..3422
                     /locus_tag="PC472_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2728092.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3657..4847)
                     /locus_tag="PC472_002778"
     CDS             complement(3657..4847)
                     /locus_tag="PC472_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2728093.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5107..5538)
                     /gene="dut"
                     /locus_tag="PC472_002779"
     CDS             complement(5107..5538)
                     /gene="dut"
                     /locus_tag="PC472_002779"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2728094.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5651..7879
                     /locus_tag="PC472_002780"
     CDS             5651..7879
                     /locus_tag="PC472_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2728095.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKFVKGKESKFDKLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7990..8475)
                     /locus_tag="PC472_002781"
     CDS             complement(7990..8475)
                     /locus_tag="PC472_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2728096.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8727..9950
                     /locus_tag="PC472_002782"
     CDS             8727..9950
                     /locus_tag="PC472_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2728097.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9954..13088
                     /locus_tag="PC472_002783"
     CDS             9954..13088
                     /locus_tag="PC472_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2728098.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            13122..14420
                     /locus_tag="PC472_002784"
     CDS             13122..14420
                     /locus_tag="PC472_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2728099.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLIALT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14709..14954)
                     /locus_tag="PC472_002785"
     CDS             complement(14709..14954)
                     /locus_tag="PC472_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2728100.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15168..17795)
                     /locus_tag="PC472_002786"
     CDS             complement(15168..17795)
                     /locus_tag="PC472_002786"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2728101.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GNVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17856..18698)
                     /locus_tag="PC472_002787"
     CDS             complement(17856..18698)
                     /locus_tag="PC472_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2728102.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18705..19343)
                     /locus_tag="PC472_002788"
     CDS             complement(18705..19343)
                     /locus_tag="PC472_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728103.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         6805 a         3090 c         2687 g         6935 t
ORIGIN      
        1 acaataaaaa aatgtagaaa gtaagtatct ctctacattt tttattgtat attattttta
       61 tattaattta attttatctt gattcagttt tataaaaaat tgttataatt gtcaagttct
      121 actctattat ttataatttc ttatatctta ttaacaacat ttaagttatt acatttattt
      181 atatatattt tttcattatc taagtcttca tcaaacattt ttattatatt ttttatctca
      241 tacttgtact catcttcatt cattctataa agcattaaat cttctagtac atctttcata
      301 tcgaagtaca catcttctaa taaataaaaa tagccttcct ctatatcttt caatttatct
      361 atagtatcca tttttttact tatatctttc ataatttcta aattttgtat atttacatct
      421 atgattgtta ataagtacat tctcacagtt tttattattt caacagaaaa atctacaata
      481 ttataatttc cttctttttt atataatgac tccttaaatt tgttaaaaca attatctaaa
      541 ttagaattta tatcgttaaa tggtggtctt attatgtcac gtattattaa aagattttgt
      601 atatctacat tatttgtgat tgcctcatcc aaaataaata aattatatgc atcatttaat
      661 gggtcatggg catctccact aaactctaag ttaacaaatt caagtaaatt tttcaatgat
      721 atatagttca tacatctcat accacaattt atatattttt cttttatatc tacaaataaa
      781 tctctaatgt tatttacaaa ccttggatgg ttattttttt tagcacttct catatctgtg
      841 ttattgaaac atgtcaaatc caaattacca aaagtatata ttcctttaat atcagaaaat
      901 atgccaagcc attttttaaa ctttctcatg acctcttcat aacttggtgc actcttcaat
      961 tcttcagaag atatatgtgt taattcttgt atatgaggat acaactcctc attacttact
     1021 ggtcttatta aagatttaaa tttatcaata ttttttgttt tttcatcata cataactgct
     1081 ccgattgcaa ttatatcaga attaaatact gctctttttg atttagaatt tggcatattc
     1141 atttcaaaat ctatatatac tcttttcaat ataaacaccc tttccaattg tttatagtga
     1201 ttctttttaa ctatttttat tcaaaatttt ctaagaaaga atgtttctcc ccatcttttt
     1261 gccaaccaag tactgaatca agatttacat tttctgctgc cttaaatata gatttttcta
     1321 catttttaaa atttgattct aagctcttta ttaagtcttt tatctcatat cttttattac
     1381 ctgttttttt agcagcaacc atacatgcac aatttaatgg ccatatacca ctattttgaa
     1441 taaatcttat aatatgttct tctcttatat aataaagtgg tcttataatt tttatacctt
     1501 caaaattagt agaattaagt ttaggaagca ttgttttaaa atttccagca cacaataaat
     1561 taagcattgt tgtctctatt acatcatcat aatgatgacc taatgcaagt ttgttacacc
     1621 ctaattcttc tgcctttgaa tataatgctc ctctcctcat tcttgcacac atatagcatg
     1681 gataatcttt tgctatttca tctgctatct caaatattct tgaatcaaat aaatgtattg
     1741 gtatatttag atactcacaa ttatctataa gtaattgtct tatatttgca tgatatccag
     1801 ggtccatagc aataaattca acatcaaaat taacttgtcc atgttttttt aactcttgaa
     1861 acattttagc cataagaata ctgtcttttc caccagaaat cgctacagct atcttatcac
     1921 cttcttcaac caatttataa tctcttattg ccttcataaa cttagaccat aaattttttc
     1981 tatatttttt tattatactt ttttctatat ctcttactgg aagtctttcc tcaaaaggaa
     2041 ctataatttc gcagcctttt cctgatagtt cactcatcta atcacctcgt atatttatgt
     2101 taataatatt ttacaaaaaa gtctaccttc atccacaaaa tactataatt tttactttaa
     2161 ttttactaaa taatttatag tttacaataa cacataaact tataataaca taaataacct
     2221 ctttctttta ttatcttttt tatttttctc atttaatatt ttattattag taacgatttt
     2281 taaatgattg aatattatac actagataga gttacctatc tatttacaac aaggaggttt
     2341 tcatcttgga aaataagact agagatatca aaaaaaattg tggtccatct ttggctagac
     2401 catacattaa taatcaaatt ttcacagaaa tgtactgcct ttctgatgca ttaaaatttg
     2461 gaactatatt tccagaacta aacttactag agtctgaaat gtacaacaaa gaattatatg
     2521 ctaagccaaa aaaatataga ggaggtagaa gataatgaaa gactcatcta gagctgaact
     2581 tatgagaaaa gttcaggaaa catcttttgc atgtgttgat atgaacttat atttagacaa
     2641 tcaccctgat gataaaaatg ctatcaacac ttataattca ttatgtaatc aatttgcgca
     2701 agctagatat gcttacgaaa ataaatacgg tcctttaact aactttgggt atgctccaag
     2761 tagatgccct tggcaatggg ctgaccaacc ttggccttgg gacagagaat ttaattatta
     2821 gacatcaagt attggaggat tgaaaattca tgtggatata tcagaaaact atacaacacc
     2881 cagttaatat aaaaacttgt gaccctagaa tggctaaatt tcttataact caatttggtg
     2941 ggccaaatgg ggaacttgct gcatctttaa gatatttaag ccaaagatat acaatgccta
     3001 ctggtaatat gcgtgcactt ttaacagata ttggtacaga agaactagct cacgttgagc
     3061 ttatatgtac tatggtttat cagttaactt ctgatgcaag cccagaagag ttaaaagctg
     3121 caggtcttgg ttcaaactat gctcaaaatg gatatggaat ttatccaaca gattcaaatg
     3181 gtgttccatt tgatgtaaga cctatagcag ttatgtcaaa tcccgtaacc gatttacatg
     3241 aggatatggc agctgaacaa aaagcacttg caacttatta tcaacttata aacctaacag
     3301 atgacgttga tgttatagat gtattaaaat tcttgggtca aagagaaata attcactatc
     3361 aaagatttgg tgaagcttta atggatgctt acgagttaga agaatctcaa aaaatgttct
     3421 aaaattttta tttgtataac tattattatt tgataattta taatttactg ttgtgttaaa
     3481 atttataaaa aatataaata taatttattt tctataaaca gagaaaataa ataaaaagca
     3541 ggtatcaatc aaatagtaac atgtattata ctatacatta ctatcaatat tgtacctgct
     3601 ttcatattat aagcaataaa gaaaaatcta tttattaggt tttataataa aaacaattat
     3661 aaaactcttc ttgcatttac atatctactt gaatagtagc tactatttat attagatatt
     3721 gttacttttt ttgatgaacc tgaagatgca tgtatcatat ctccatttcc tatgtatatt
     3781 ccaacatgac ttacagaacc attatttgaa ccttgtgtat caaagaatac taaatctcca
     3841 cttctaaggt ctcctctatt tacatatgtt ccatacttac tttggtctct agatactctt
     3901 ggtatactaa ctcctacaga ttttttcata acgtactgtg tgaatccaga acagtcaaaa
     3961 ctatttggac cttctgctcc ccatacatat ggttttccaa gtagtgtctt tgcaaaactt
     4021 attacactat cagctccttg acttggagaa ttagtcgaat aagatggaat tgaagtatca
     4081 acagaacctt tttcagcaag gtattgactt gacacatatc cagtttgatt gccataactt
     4141 acttttgtcc atcctccact ttcagaaata cttccaacct cagtaccata acttaatgta
     4201 gctattttag aatgacttgt acctggtcct gttctaacgt tcaatgactt tgctgttaca
     4261 acctttgtac cctttacaag gtcatttgaa ctgttttcag cattttcatt tgtagagtca
     4321 tttacactag ttcctaaata tttacttgaa acataaccta ctctaccatt agaacttatt
     4381 tttgaccatc catttgactc agatatgtat ccgacttcag tcccatatcc tagtgttgat
     4441 attttagaac tacttgtact tggtcctgtt ctaaaattta atccttttgc tgttacaact
     4501 tttgtcgact taactgaagt atcactactt tcattgctac ttccaaggct atttattgct
     4561 acataatttc catgcacata acctgtgttt ccattatatt taacttttac ccaagaacct
     4621 tcttttccta cgtactcaac tttatcacct ttgtaaagtt tccccattga ttcatggttt
     4681 gtacctggtc cttttctaaa atttaccgca ttccctgtta ttgttctgct ttctatactc
     4741 acactctcta cttcagactc attagcatat gctaaattcc ccatagtagg cattattatc
     4801 gcagttgcca ttatactggc tgctattctc ttttgattaa aattcataac tttccactca
     4861 tctcctcttt tctactatct ttatttcact tcaaaatata aaagaaataa ttgacactat
     4921 caaaatatca attatttctt ttatattaat attattacaa atttgtaact atttgtctat
     4981 agtaatattt gcttaccata aattaccatg tataaatttt aatctttaat agcctgtaat
     5041 ttagtaatat agccatcttt tacctttttt aaaaatggct atatttttta acttttttgt
     5101 aactttttaa aatccagttg agccaaaccc accttttcct ctttcggagt cacttaattc
     5161 ttttacttct tctatgttaa tatcatagat aggttttact atcatttgag caattttcat
     5221 atgcttttca accttaaaat catttttacc atgatttatt aatataattt taagctctcc
     5281 tctgtatcct tcgtcaatag ttcctggtgt atttaataca gtaacagaat gctttaaggc
     5341 aagcccactt ctaggtctta cctgcgcttc tgtcatagta ggaagctcta tacatatccc
     5401 agttttgata agtttagtct ctccaggagg tattacaacc tcttctatag aatacaaatc
     5461 cataccagca tcacctttgt gagcaaaatt aggtattata gcatcatcat ttaatttttt
     5521 tacttttaaa ttgtacatct tatatctcct ttcaactttc tataaatatt ataacttaaa
     5581 tttcgacctc ttgaagtttt ttattttttt gtggtaatat aattattagt gttttaaagg
     5641 aggatttttt atgaaacttg tacttgctga gaaaccatct gtagcaaaaa ctatagcatc
     5701 atttttaggt gcaaagacca gacaagatgg ttactttgaa ggaaatgatt atataattac
     5761 ttatgcagtt ggacacttag tatcattata tgatatgaag gattatgata aagataaata
     5821 ctctggctct tggaaaatga acaatttccc atttgttcct gaggataagt ttaaatttaa
     5881 gattgatagt tctaaaacta aacaattcaa cactgtaaaa aagcttttga atagaaaaga
     5941 tgtagaatat gtaataaatg ccactgacaa tgaccgagaa ggagaactta tagcattttt
     6001 aatattttta ctggcaaaaa ataaaaaacc agtaaaaaga atcttggtaa atgaatggac
     6061 accagaagat attacaagag gtataaaaaa cctaaaagat gaagatgaaa tgagaaatct
     6121 tcaagctgct ggatatacta ggcttattac tgactggttg attggaataa atttcacttc
     6181 tgttgctaca ttgaaatatg gaaatgggaa actattaaac ataggtaggg taattcttcc
     6241 aacagttaaa ttagtctatg atagagatat ggaaatacta aactttgttc ctaaaactta
     6301 ttatgaaata gaagggcatt ttaaagctga agctggtgaa tataaaggta aatttgttaa
     6361 gggtaaagaa agtaaatttg ataaacttga agatgcaaat aaaattatag cttcaattac
     6421 atctgaaact ggtaaaatac tagataaaaa agttactatg tcaaaggaat atgcaccaaa
     6481 actttttagt ttgacatcac ttcaaggata tataacttct aagtattcca attttacatc
     6541 tgataaggtt ttaaatgtct gtcaatctct ctatgagggt aatggtaagg gtggatacat
     6601 aacttatcct agaactgatt ccattttctt agaagaaagt ttggcttcaa aagcttctca
     6661 aactttagat aaattaaagg ttggtcttga atatgaaaat aaaattaaat tttctaaaac
     6721 taaaagagtt tttgattctt ctaaagtaga ctcccatagt gctataattc caacttatat
     6781 aattccaaaa agtttatcta aggatgaaca attagtttat gatgccataa aagatagatt
     6841 tgtagcaaac tttatgcctc ctgctgaata tgagaataca gaaattaaga ctgaagtaga
     6901 taattgtact ttcttaacaa aaggtaaggt acttaaatca aaaggatatc ttgaagtata
     6961 taataaggaa gaaaaaaacg atttacttcc acttgtaaat aaagatgatg ttgtagatgt
     7021 gttagaaata aaaccattaa ctaagcaaac tactccacct aaaccatata ctgaggacac
     7081 ccttttaaag gctatgaaaa attgtggtaa aaatgtgcct gaagaagata caacagtgct
     7141 atctggatat tcgattggaa catcagccac acgtgctgat gtgttaaaga agataagtca
     7201 agtaggctat gtaaagaaaa aagggaaatc ttattctata acagaccttg ggaaaaattt
     7261 agttgaaatt tttcctgtta aagacttatt tgatgtagat tatactggaa aacttgaaaa
     7321 aagtttaagc gatattcaaa aaggaaagta tacaagaaaa gaatatctta ctaatatcat
     7381 tagttttata tatcaaaatg tcaatattat aaaacaagat gcttcaaaaa atataaacac
     7441 agaagcatat actttcgatt caaaaactaa aaaatttact aaaacaaaag aaaaaaaaca
     7501 gaaaaaagat gtaactaata ctgaaaaaga agttgctata tcaaaggatt ctaataacaa
     7561 aaaagaagtt aaaaatggtg atgaaaatca atctcttggc aaatgcccca tttgtcaagg
     7621 tgatgtacta gaatttgata aaggctttgc ttgtaaaaat cataaagaat gtaaatttgt
     7681 tatttggaaa aatgataagt atttaagtct ttataaaaag aaagttaaca aaacaatggt
     7741 caaaaatatt ttaaaaaaag gtgaaactaa agtaaaatct ttaactgcta aaaacggtaa
     7801 taaatttgat gctacattaa aatatgataa aaatccagat actggctatt tcaattggaa
     7861 aatagagttt gacaactaaa tattcataag aaaagaaata gcagtagtat aataatctat
     7921 attatattac tgctatttct tttcttatga agttttaact tttttaatag tgacttttac
     7981 aatcacactc taacctaatt tctttactat ttctgataac tttaatgtat gttcatctaa
     8041 taaagttagt tcctcttcag aaagattaga taattttttt actagagatt ctttgagaat
     8101 agcttttact gcatcaaata atttaaatgc tttatctgtt aattcaaccc taagaatcct
     8161 tctatctttt gggtcaggaa atttattgac taaaccatgt tccactaatc tgtcaataat
     8221 cggagtcata tttgatttta aaattcctaa attagaagct atttgagaaa ttgatgatga
     8281 atttgatgtc ataagataaa gaattacctt agcatgtgat ggagccattg gacattcttc
     8341 aaaataagct ttcattatat cagagttatt ttgaaacttt tttaacaaat cgctttcttt
     8401 aaatatatta ttttcaagca caaataaaaa atctaaaaaa gagtccgcta ttttaagtat
     8461 attgttgtta tccatatttg taacatccct tcaatagata atataataaa acattgttgt
     8521 ttaatatagt ataaattata aactattaat aaatttaaaa caagaaaaag ttattgactc
     8581 ataataatta taaatttata atcaaattag agaattataa attgccaata aagggaagtt
     8641 ttttaattag tctaaaacta aacttaaatt ataagacact ttaaattcta caacaagcta
     8701 gtgtgcaata tataatagga ggagatatga aaagatattt aatagttttc ttaatgtgta
     8761 gttcactgtt tttagttgga tgtggaaaaa cagaagaatc tgaacctgag aaaccaatag
     8821 cagtttctgt tcaaaaagca ataggtggag aaattgaaaa cacaaattct ttctcaggaa
     8881 ctacaaaggt taaagatgaa acagctgtta ctgcacagac tgttggcaca gttcaagaag
     8941 tgtatgtaaa acttgggcaa aatgtaagaa aaggagatga attattgagt ataagcagtc
     9001 cagagctaga aaatagtgta aagcaaagta aagcctcact agatttagca aaagcaagtt
     9061 attcaagtgc cactggtggt tcattagagg cacaagtaaa tcaggcaaaa acagctcttg
     9121 acaatgcaaa gattcaatat gaggagtctc aaagaaatta tgacaataat aagattcttt
     9181 atgaacagga agtcattagt ttagaccaat ttaaaaaaat agagttttct ttagagcaaa
     9241 caaaacaaca gttagattct gctcaaagag catacgatac agcaacttca aaaagtatac
     9301 ctcaagctaa agcacttgct aaaaaacagc ttgaccaagc tcaagtatca tataatttag
     9361 caatgagtaa tttagacaaa ttaacattaa catctccagt agatggtaca ataacagcta
     9421 aaaactttga ttcaaaggaa atgataacac aatcacaacc agcttttatt atttctaatc
     9481 ctaatatact agaagttgat ttaaatgtag cagaatcaga tataggtaaa tttaagaaag
     9541 atggaaatgt agatgttata attgaagacc aaaggatttt aggaaaaata gattatgtac
     9601 cttcagtagt tgaccctcag acatctttat atcctgttaa agtacttgta aataatacaa
     9661 acaacaaatt taaggcaggt atgtctgctc aagtaaactt aagtgttgaa aaagaaaatg
     9721 gtgctgttac agtacctaaa aaagcaatat ttgaagaaaa tggtaaaaaa tatgtataca
     9781 tagcaacaaa agacaatata gcaaagaaac acttggttca aactggaatt gtaacagagg
     9841 ataaaataga aattaaaagt ggtgtaagtg ataaagatac agtgattatt ggagggataa
     9901 gtcttatttc tgacggaact aagatatttc ctgtagaaaa ggagaagtag actatgaatt
     9961 taacacagac ttccgttaaa cgtcctctca caatcataat ggtcttttta gtattaatag
    10021 tttttggtgg tattggatat aaaaaaatgt caataaatct aatgcctgat atagaaatac
    10081 cagttgtaat ggtcatgact acttggacag gtgctggtcc tcaagacgtt gatgaacaag
    10141 ttagtcaaaa agttgatgaa agtttatctg ccgtttcaaa tgtaaaatct actatatcaa
    10201 gttcacaaga aagtgtttct atggtagttg ctcagtttga atttggaact aatttggatg
    10261 aaataatgaa tgatgttagg tctaaagttg atgcattaca gaccagcctc ccagatgatg
    10321 cagctaaacc aacagtttta aagctggata tgaatgcaca ggctataggt caacttgtca
    10381 tatctggagg aaatgaaaat agttcacaag ccttaagaaa atacgctgaa gatgtaattc
    10441 agcctaaaat agaatccata gatggtgtta cttcagcaga ccttaaaggt ggaaaaaaag
    10501 cacaggttaa tgtaatagct gacccagctg ttctttcaaa ttatggagtt agtttatcca
    10561 ctataaaagg tgtcttatcc tctagtaata aaacattccc ttatggttca attactcaag
    10621 gtgaggataa aatagtttta agagcaattg ataaattaga atccctagat gatataaaac
    10681 agattcaaat tcctgtaaaa ggaggaaata cagttagatt agataatctt tgtagtgtag
    10741 attatggata tgcagacaaa gatagtattt atagatacaa tggaaaagac agtttggtaa
    10801 tagatatttc taaacaacaa gatgccaaca ctgtaaaagt tatgcagagt gcatacaaat
    10861 acgtagatga attgaataag gaaaatcctc aatttaaaat taaaattgct aatgatacaa
    10921 gttcatacat aagtgaatca ataaacagtg taatgagcaa cttgcttata agtgctgtta
    10981 tcgcatttat tgtaatattt gctttcctaa aaagtgtaag agcttcacta gttgttgcag
    11041 ttgcaatacc aatttctata ataggtgcta tagcaattct ctactttact ggtgaaagtt
    11101 taaatctcat tactgcaagt tcattggtaa tttcagttgg tatggttgtt gataatgcta
    11161 cagttgtaat tgaaaatata tttaaatata ggaaaagtgg cagattgagt cttgatgatt
    11221 gtgctattga gggaacaaga actgtaacaa atgcaatact tgcatcaaca ttgaccacga
    11281 tagcaatatt cttacctatc atgtttacag aaggtatagc taaaataact tttggggcat
    11341 taggtaaaac actaatagct tcactagcat tctcctttat aggtgcaatt acactcgttc
    11401 ctagtgtatt tgcaaaatta agtagaggaa aaaatagtca aaagatgcaa gaaaaagata
    11461 gtcctatatt tgataaggta agtgaagcat ataaaaaatt attaagtgta tgtttgagac
    11521 atcaacgctt agttgtaata tcaagtttac ttatttttgt agcttcctta tttggaatgg
    11581 gatttatagg tatggacttt atgcctgcag cagacaaggg tgaattatct atttctatag
    11641 aacttccaaa aggtcttagc ctagaatcca atgattacta tgtatctatg actgagaaaa
    11701 aagtctcaga tattccagaa attaaaactt taataacaac tttgacatca gattcaaata
    11761 atagtaaagc aagtatagca attgaattag aatctcaaaa aaatagaaaa agaactactg
    11821 atgaaataga aaaagagata attgatagag tagctactgt accagattgt aaaataaatg
    11881 taagtcaaaa cagtagtgtt atgggtggag ctggaggagc agacttcaca ttagaaatga
    11941 aaggtcctga tttagatgta ttaaaaatac tgggaaaaga agctgaaaaa gaatttagct
    12001 ctataaaagg atttagaaat gttgaaactt ctcttacaga tacaacacaa gaagctcaat
    12061 ttactataga taaacataag gctcaagaat atggtgtaaa tactgctgaa attgcaggca
    12121 tgttacgtac agcaataaat ggtgactccg taaccactgc tactattgat gattacaaag
    12181 tggatgtaaa cctaaaattt aaagcagata ctattgatag cttagaagat ataaagcaac
    12241 taaaagtaaa ctctgcaact ggaaaagaag ttcctcttgg tgctttttca gatataaaaa
    12301 tggcagatgg tctacaatct atatctagga ctgatggaga tttttctgta aatataactg
    12361 ctacacttga caatttagat gctggtactg ctacaaaact agcactagaa aaaacgaata
    12421 aaatggtaat tcctaaagat tactctatag gtgttggtgg tgaaacagaa atgatgaatg
    12481 aatctatggc aagtcttgga ttttcaatgg tcatagctat agcattagtt tatatggtaa
    12541 tggtagctca gtttgaatca tttagtaaac catttataat catgttctgt gttccatttg
    12601 cattcgttgg agttgtgctt tcgcttctag ttagtggtca aagtttaagt actgtcggta
    12661 tgctaggtat aatcttactt atcggtattg ttgttaataa tggtatagtt cttattgact
    12721 acatagagca acttagaaaa acaaatcgta acaatgattt aattggtttg gtttcaattg
    12781 gttgtgcagc aagattaaga ccagtcttaa tgactacagc aacaacaatt ttaggtatgt
    12841 tgccaacagc actatctctt ggtgaaggtg gagaaactat gcaaccacta gccatagtta
    12901 taatgggtgg tctaactgta tctacactta taactttggt gcttatacct actatatact
    12961 taatatttga taaatgggaa aataagtttt cagacaaaat gcctaaaata tttggtaaga
    13021 taacagataa aataggaagt ataaaacgta aaataataaa gaaaccaaaa ttgaaagcta
    13081 aagtttaaat agaatggagg gtgattgtat tgaataaaaa aatgagcaaa atagttgcaa
    13141 ttggctgtgg tgttggtata ataactgcca atttaatccc agtttacgca gataataata
    13201 cttcttctat aaacaaagtt gataattcta ttttaagtga ctcaaataaa agaacatctg
    13261 aaaaggaaat tttgactgct agagaagctg ttagtgctgc acttaaaaat agtgaaaaat
    13321 taaaaatgaa atcagaagaa ataaaaatgt taaaagaaaa gttagaagta caagatgagt
    13381 ttgatagttt tactggaagt gacaactcgt ttccatacga ccaaattgga cttcttaaaa
    13441 atcaaagtga acaggctaaa ggctttatgg aggaccaaat agcaaatgat attactaata
    13501 aatttaacga tttagtttca agagaaaatg agctagacaa gataaaaaac aatcttgaaa
    13561 taaaaactaa agaaattaaa gatatgaaat taaaaaaaga tttagggtta gtaacatctt
    13621 tagaaactga atcagctgaa cttgaacttc aaacactaca aaatactcaa aaagctaaac
    13681 ttcaagaact aaaaaacaat caagactact ttaagttgtt gactaatata gatttaaata
    13741 attatcaatt ggataaagaa tatagatttg aatcttttag agtaggtggc tcagttgatt
    13801 catatatgga aggtaaggtt aatgaatatt taaaatacga ccaattgatt ttagaacgta
    13861 cagaagaaag ctttaatgat aaagatgaga acaaagcaga tttaccagat agacctaatc
    13921 ttacaagacc agttgctcct acaaaaccag agcaaggtaa cctaactgat gatgaatata
    13981 aggttcttac ggataagtat gaaaaagact acaagagata tgaaaaagaa gttgaagcat
    14041 acaatcttga aagacagact tatgctgctg gccttactac ttatgcaaat tatcttcaac
    14101 aaaaatttaa tactgaaaat ggtctagtaa cattagaaga cagtaaaaaa gctcttaaaa
    14161 aagggttaat tgatagctat gctcaattac ttgctttaga agatacaatt cagattacta
    14221 aaaaacaact tgatttatca gaaaaacaat taaaaaatac taaattaaga tatgatttag
    14281 gattaattgc tctaactgat tataaaaaac aagtggtaag taatcaagat gctaaaaata
    14341 gctatgacac tcttattgtg aattataact cactaaaaaa tggtatagaa aaaccttgga
    14401 tacttaatac tggaaaataa ttctggtaaa tagaaagatt actttaacat acgtttgatt
    14461 aaaaaatagt tatgcacact cgtggcttta gtcatggaag attcagaagg caaataatag
    14521 aaataggtta gtaaaaatat atttacttat aaaactgtat acttttatag aggctatctc
    14581 aaaatagaga taagcctctt ttttattgtt taaattataa aaatatattt gattccatac
    14641 aaaaagagtg ccttatgaac taaaaagttc attttgagac accctcaaaa gcttaactct
    14701 aaatttaatt attttttatc aaatgtctca tctattttac atctcaccat gttaacaaat
    14761 tcagaaatat tatatcctct aagtttgcat ccacatttag gacaataatc cagtttccta
    14821 gataccaaag ccctacaact aggttctgga catcttattt ttagactctc atctgctact
    14881 ttaaatccac atcgtgggca tatactacct ggacgtgatg atacttcctt tccacattta
    14941 ggacactttg tcaactttat cccctccagt aaactcaatt taattgatag ttgaaatttc
    15001 tctctacaat aatatatgca atataattaa atacgtttca cttacaacta tataagttaa
    15061 atttttataa taaaaatccc ctatattcaa tttacaaata cttaatttga tacattgaac
    15121 aataggggat tttattttta tttagttaca tattttgcag tctattatta tctaggattt
    15181 tttatagatg cttgagctgc tgcaagtcta gcaattggca ctctaaatgg tgaacaagat
    15241 acatagttaa gtcctacact ataacagaac tctactgaag atggttctcc tccatgctct
    15301 ccacatattc ctagttttaa ttctggttta acttctctac ctaatttagc tcccatctta
    15361 actaacttac caacaccatt ttggtctagt acttggaatg ggtctttttc taatatttct
    15421 tcatctacat attgacctaa gaatttacca gcatcatctc ttgaatatcc aaatgccatt
    15481 tgagttaagt cattagtacc aaaactgaag aagtctgctt cttgtgctat ttcatcagca
    15541 gttaaacaag ctctaggtat ttctatcata gtacctacag tataaggaac ttctatacct
    15601 tcttctttta ttatagcgtc aacagtttct actatcattt ttcttattat ttttaactca
    15661 tttacttctc caactaatgg aaccattatc tctggagaaa ctttaacacc tgctttgatt
    15721 gcttctatag caccttgtat tatagcttta gattgcatta cacatatttc aggataagtt
    15781 attgcaagtc tacaacctct gtgtccaagc attgggttaa actcatctaa atcaactatt
    15841 ctcttcttaa tatcacttac ttttattccc atagattttg aaagttcttc tatagtctca
    15901 tcatcatgag gcaaaaattc atgtagtggt ggatctagta atcttatatt aactggtttt
    15961 ccatccataa ctttaaataa ctctacaaaa tcttctcttt gcattggtaa tattttttca
    16021 agagcttttt ctctttgttc tactgtatta gataatatca tttctctaac tgctggcaat
    16081 ctagcttcat caaagaacat atgctcagtt ctacatagac ctataccttc tgcaccaaac
    16141 tctatagctg cacgagcatc tctaggatta tctgcattag ttctaaccat catacatttg
    16201 tgtttgtcta cccagttcat taatttttca aaatttccag ttaaagatac ttctgttttc
    16261 ttaacatttc ctaggtatac atttcctgta gaaccatcta tagaaatgta ttctccttct
    16321 tttataacta catcatcaag tgctcttatt tctttattaa actcatcaac ttttatttct
    16381 ccacatccag caacacaaca tttacccata cctctagcaa caactgctgc atgtgatgtc
    16441 attccacctc tagctgtaag gattccttct gctttaacca ttccttctat atcttctgga
    16501 gatgtctcta gtctaactaa aactactttt tctccttttt catttgcttt tactacatca
    16561 tcagcattga aataaacttt accacttgct gcacctggtg atgctggtaa accttttgct
    16621 ataaccttgg cttcttttaa tgccttatct tcaaattttg gatgtaataa ttggtctaat
    16681 tgattaggct caattctcat gatagcctct ttttcatcaa ttattccagc ttcaactaaa
    16741 tcaaccgcta catttattgc tgctgctgct gttctcttac catttctagt ttgtagtata
    16801 tataatcttt cattttctat agtaaactct atatcttgca tatctttata atgaccttca
    16861 agtatatgag taatttttac aaattcatcg aatacagctg gcattacatc tgcaagagtt
    16921 gatatatttt gaggagttct tatacctgca acaacgtctt caccttgtgc attcattaaa
    16981 aattcaccaa ataatttatt ttctcctgta gctggatttc tagtaaatgc aacacctgta
    17041 ccactagttt ctcccatatt accaaatacc attgattgaa tatttactgc tgttcctaag
    17101 ttatgagcta tatcattcaa tttacggtat acgattgcac gaggattatt ccatgatcta
    17161 aatacagcct ctattgcaag cattaattgc tcttttgggt cttgtgggaa atcatttttt
    17221 atttcttttt tataaattgc tttaaattct tttactattt cttttaaatc atcagttgta
    17281 agttctgtat caactgtgta tccctttgct tcttttactc tgtctaatac attttcaaat
    17341 ttatattttg gaacttccat agcaacatca gaaaacattt gtatgaatct tctgtaacta
    17401 tcatatgcaa atctttcatt ttgtgtagcc tctgctaagc caacaacact attgtcatta
    17461 agacctaggt taagaattgt atccatcatt cctggcatag agaatactgc accagaacga
    17521 actgatacta ataatggatt cttattgcat cctaatgttt tatttaagtc tttttctaaa
    17581 gtagctaaat gctcttctat ttcttttatt atctctgctc ttatactttc attgttaaca
    17641 taataatcat tacatgcttc tgttgttata gtgaatccag gagggacagg taagcctatc
    17701 ttagtcattt ctgctaaatt ggcaccttta cctcctagta aagatttcat gtctttgctt
    17761 ccttcaccaa aactataaac atacttagtt tccattgtta aatcccccat taatcttttt
    17821 attttaattt atatcatttt tattaataga cattattaaa tttctatttc tttgtatatc
    17881 tttagaccat tttctttcat tatgtctaaa attatacctg ctgtttcttc tattgcctta
    17941 tttgaaacgt ttattactgg gcatccaatt cttttcatta tctcttctga ataatctaac
    18001 tcctgaagta ttctttctaa attagcgtag tttgctttac tggacaatcc taaagctttt
    18061 aatctttggg ttcttatctc attcagcttt tcaggtgaat tagtaagacc tattattttt
    18121 ttagtatcta tctcaaacac ttctttagga attggtattt ctggaactaa tggaacatta
    18181 gctactttta tatttttatt agctaaatac atactcaaag gagttttaga tgttcttgat
    18241 attcccacta aaattatgtc tgcttgaaga acacctctag ggtcttttcc atcatcatat
    18301 ttcactgcaa attctatagc ttcaactctc ttaaagtaag attcatctaa ttttctaatt
    18361 ataccagctt ctcttttagg ttttcttcct gttctcttgc ttatttctct tagtatatct
    18421 gtcattaaat ctatacaact aagaccttct ctttcgcaat atctttcaac aatactgcat
    18481 aattcttcat caactaatgt atatacaatt aaagcgtttt cagcttttgc attctccaaa
    18541 gtttcaagta gtactccttt attaatcatg tatggaaatc tttttatttc ataagtttca
    18601 ttcacataaa actgagacat acatgccttt gcaacttgtt gtgcagtctc ccctactgaa
    18661 tctgatactg catatataat taggtttttc accagcatac ctccttaatt gtcaactatt
    18721 tctaagaata gcttagttat gttggttttt gatattctcc caacaactct catatgattt
    18781 ttgtcttctc ccttatattc aacaatcgga atcgaatcta cttcatgttc tattattttt
    18841 cttgaagcta ataaaacact tgctccttta gttaaagtga ctacatttgg agttcttgtc
    18901 attatcatac ctattggcat tttatttata tcagaacctc ctatagttgc ttttagcaag
    18961 tcttttctgg aaacaactcc acataactct tcattctcat ctataataac tatactccct
    19021 acatcagata aaaacatagt gactattaca tcatatatac tttcatcctt cttagctagt
    19081 acaggcatgc tcattatatc ctctactgtt ttttctttta cacttttacc aattaaattt
    19141 atttcactaa ctcctgaata aaaatatcca acttttggtc ttgcgtctaa tattcctgtc
    19201 attgttaata tagctaaatc agatctaagt gtagcacgag tcacattcaa gctggaggca
    19261 atactttcac tcgttattgg ttcattttcc ttaactatat ctataatttt tagttgtctt
    19321 tcattaagct gaataataat caccttcttt cattgtgcta tactttttat cttttcgcat
    19381 cttatatagt atattatata actatatata ccattctgta aacagatttt ttattgaata
    19441 cgatatattt tttactaagt tctctcgctt aaagatattt attatatatt aatgggggat
    19501 atttctttaa ttaatag
//
LOCUS       DAMJYC010000074        19387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJYC010000074 DAMJYC010000000
VERSION     DAMJYC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.075"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(76..591)
                     /locus_tag="PC472_002789"
     CDS             complement(76..591)
                     /locus_tag="PC472_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2728104.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(954..2024)
                     /gene="leuB"
                     /locus_tag="PC472_002790"
     CDS             complement(954..2024)
                     /gene="leuB"
                     /locus_tag="PC472_002790"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2728105.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2031..2522)
                     /gene="leuD"
                     /locus_tag="PC472_002791"
     CDS             complement(2031..2522)
                     /gene="leuD"
                     /locus_tag="PC472_002791"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2728106.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2537..3814)
                     /gene="leuC"
                     /locus_tag="PC472_002792"
     CDS             complement(2537..3814)
                     /gene="leuC"
                     /locus_tag="PC472_002792"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2728107.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3771..5513)
                     /gene="leuA"
                     /locus_tag="PC472_002793"
     CDS             complement(3771..5513)
                     /gene="leuA"
                     /locus_tag="PC472_002793"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2728108.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5637..5883)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6031..6477)
                     /locus_tag="PC472_002794"
     CDS             complement(6031..6477)
                     /locus_tag="PC472_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728109.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6498..7217)
                     /locus_tag="PC472_002795"
     CDS             complement(6498..7217)
                     /locus_tag="PC472_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2728110.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7335..8609)
                     /locus_tag="PC472_002796"
     CDS             complement(7335..8609)
                     /locus_tag="PC472_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2728111.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8809..10176)
                     /locus_tag="PC472_002797"
     CDS             complement(8809..10176)
                     /locus_tag="PC472_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728112.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10426..10890)
                     /locus_tag="PC472_002798"
     CDS             complement(10426..10890)
                     /locus_tag="PC472_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2728113.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10895..11524)
                     /locus_tag="PC472_002799"
     CDS             complement(10895..11524)
                     /locus_tag="PC472_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2728114.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11558..12445)
                     /locus_tag="PC472_002800"
     CDS             complement(11558..12445)
                     /locus_tag="PC472_002800"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2728115.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12474..13712)
                     /locus_tag="PC472_002801"
     CDS             complement(12474..13712)
                     /locus_tag="PC472_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728116.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFITLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14096..15238)
                     /locus_tag="PC472_002802"
     CDS             complement(14096..15238)
                     /locus_tag="PC472_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2728117.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15379..16245)
                     /locus_tag="PC472_002803"
     CDS             complement(15379..16245)
                     /locus_tag="PC472_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2728118.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16579..18126)
                     /locus_tag="PC472_002804"
     CDS             complement(16579..18126)
                     /locus_tag="PC472_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2728119.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18119..19255)
                     /locus_tag="PC472_002805"
     CDS             complement(18119..19255)
                     /locus_tag="PC472_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728120.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
BASE COUNT         6401 a         3528 c         2008 g         7450 t
ORIGIN      
        1 atttttattg tatttttgaa ttttattttt tctaaatata aaatttatgt tttaaaatat
       61 aaaattttta tttaatcaaa gttcaatata ttccactttt tctttcgatt cttcaaaaaa
      121 ccattcttca ttagaaaaat caccttctgc aatttgtcca ataggcattt tcatatactt
      181 tccagttgct gtcactgcta tatctccatt tggtagaatt atttctcctg taccttcaaa
      241 taattttctg ttttctttag ttattcttcc aacaactttt atagtagcat ctgttggtac
      301 tggtttctta tatttcaact ctaaagatac tgttactccc catatttggt catcatttat
      361 acttacagct cttccaatag tctcatccaa tattgcagca gacatacctc catgaactct
      421 cccaggataa ctttgatgcc aatcctttgt attacaaatt gatacaagct ctccattttc
      481 aagttcataa aaatctgctt taagtcctag ctcattttgt gttccacaaa ctaaacaaga
      541 tttactacta ttttgcttct tcaaaacttt atactttgcc aaaaaatcca tctcctaacc
      601 gacattagta gtcgcttaat attaaataat tctaaaatat attatataat attaataaaa
      661 tccctgcaaa cttctaataa taaaaatagg ctacttcaat atgataagtt taatttaaag
      721 tattattttc caaaaaataa ctttatcaaa atagaaactc aaagtagata taaacctctt
      781 tttcgttatt aaaatagtaa aaatatattt gacttaatat aaaaaagagt gtctcattaa
      841 ctaataaagt ttattttgag acacccctca taaaacaaat tatactacaa tagattttta
      901 ctaattctac aagttatata tttaaattat attattcaaa ttaaactata attctaaatt
      961 tcatttacta ttaattcgcc catttccctt gttcctacaa catttcccac accattatag
     1021 atatcaatag ttctataacc tttattgagt acactttgta cagcatcttc aatacactta
     1081 gcttcttctt ctaagttaaa actatgtctt aacatcatgc ttacagatag aatagtcgca
     1141 attggattta caatatcttt tccagctata tcaggtgcac ttccatgtat tggttcatac
     1201 ataccaaaac tatcttctct aagtgatgct gatggtaaca tacctataga acccgtaatc
     1261 atagatgctt catcagatat tatatctcca aatatattat tagtcactat tacatcaaat
     1321 tggctagggt cttttacaac ttgcatagca gcattatcaa catacatatg tgacagttct
     1381 acatcttcaa actctttagc taagtcttcc attatacttc tccaaagtct tgaactctca
     1441 agcacatttg ccttatctac acttataagt tttttatttc tatttctggc tatttcaaaa
     1501 gctctcttac caattcttct tatttcattt tcgttatata tttctacatc ataagcattt
     1561 tcgataccat caatgatttt tcttccacgt tctccaaaat aaatgccccc tgttaattcc
     1621 cttacaacaa caaaatcaat gcctttctca actatatccg ttctaagtgg tgaagcttct
     1681 tttatacttt catacattgt agctggtctt aaatttgcaa ataaacccaa tgactctctt
     1741 aattttaaaa gaccagactc tggtctttta tctcccttac atttatccca ctttggtcct
     1801 ccaacagcac caagcaatac agcttcgttt tttcgacaag tttcaacagt tttattaggt
     1861 agaggttctc cagtttcatc aatcgcgcat cctccagcta atacatattc acaatcgaat
     1921 ctatgattaa atttacaaca aattttattt agaaccttta taccttcatc tataatttcc
     1981 ggtccaacac catcaccttt tatgactgct atattacaat tcatacttca ctacccccta
     2041 tttgctttta ttgagttaat taagccttca tttaatataa tattttgcat aaactcagga
     2101 aatggttctg ctttatattc ttttcctttt gttatatttt ttatcacacc tgtagaaaaa
     2161 tctacttcta tattatcccc ttcatctatg ttattggcag cttcttcaca ttcaagtata
     2221 ggaagcccta tatttataga gtttctaaaa aatattctag caaaagtaga agcaatcaca
     2281 caggatactc cactttcttt tattacaatt ggtgcatgtt ctcttgatga accacaacca
     2341 aagtttttag ttgcaactat aatatctcct ttttttacct tagaaataaa tttatcatca
     2401 atatcctcca tacaatgcgt tgctagttct ttataatcag ctatatttaa atatctagct
     2461 ggtattatta catctgtatc tatattgtca ccgaatttaa aaacacttcc attggcaatc
     2521 ataaatttta cctcccttaa attatttctg atggttttga tatttttcca gttatagctg
     2581 atgctgctgc tacagctggg cttgcaagat acacctctga atcaacatgc cccattcttc
     2641 ctacgaaatt cctatttgtt gttgaaatgg atttttctcc tgctgcaagt atacccatat
     2701 gtccccctag acatggacca caagttggtg tagaaactgc tgctcctgcc tctataaaag
     2761 tggtaatata ccctttttct atagcttcaa gatatacttt ttgagttcca ggaaatatca
     2821 taactctaac tccatctgct acttttttgc ctttcattat ctcagctact acttcaaggt
     2881 ctgatattct tccatttgta caagagccta ttacaacttg gtctatcttt atatccttat
     2941 ctatctcatc tactgttcta gtattttcag gaagatgagg aaaggctact gtttctttta
     3001 gttcagctaa atctatttcg tatacttcat catagactgc atcttcatct gcttcatata
     3061 tatttacatc ttttgtcata gttgaacact tatgtgaatt tatatattcc atagtcttat
     3121 cgtctactgg aaaaattcca ttttttgctc ctgcttctat tgccatatta caaatagtaa
     3181 atctatcatc catagataag tattctactc catctccaca aaattccatt gacttgtaaa
     3241 gtgctccatc aacacctatc tcccctatta tatgaagtat tatatcttta ccacttatcc
     3301 atttatttgg tttattcttc aaaacaaatt ttattgctcc aggaacctta aaccaacatt
     3361 ttcctgtagc catacctgct cccatatcag ttgaacctac accagtagaa aatgctccta
     3421 atgctccata tgtgcaagta tgagagtcag cacctataat tacatctcca gcagttacta
     3481 tacctttttc aggaagaaga acatgctcta ttcccatatc tcctacatca aaaaaattta
     3541 caactccttt tgattttgcg aaacttcttg taaatttaca ttgttctgca ctctttatat
     3601 ctttattagg tgtaaagtgg tctaaaacca tcactacttt agtcttatca taaacatctt
     3661 caattcctaa tttttcaaat tctctaatgg ctactggtga tgttatatca ttacctaaaa
     3721 ctacatctaa atctgcttcg attaattgcc ctttcttaac ttcatttaat ttagcatgtt
     3781 tggctaatat tttctgcgtc atagtcatcc ccatttttta tttcctcctc aatataactt
     3841 ttatttattg cattcataag tgcattaata gctgctgtat ttatgttttc cgatatactt
     3901 acaccaaaat agacttcatt attgtgctct attcctacat aagctacccc tcttgaatgt
     3961 gacccttcct ctaatgcatg ttcccagtaa tatctaaatt tataatcttt catgccacat
     4021 tgctttagtg cattattaaa tgaatctaca ggtccatttc ctataccaac aatcctttgt
     4081 tcatttccca tataacttat tcttgcagtc atctcaatca tgttagtatc attagaatca
     4141 tcattttcat agtttaattc atccattgat tttattttat acttcttaac tttataaggg
     4201 ctctcaactt caatatattc tttcttaaac aaattaaata ttgcttcact actaagttct
     4261 tccccaattc tatcagattc cattttgact acatttccaa aatcagaatg catattctta
     4321 ggtatcataa atccataatc tgtttccatt atataaacag ctcctccttt tccagattgg
     4381 ctatttatcc ttataatttc tttgtattct cttccaatat catgagggtc tattggtaaa
     4441 tatggaactt cccagtaatc attatctctg tttttcattg ctatcatgcc ttttctaata
     4501 gcatcttgat gtgaacctga aaaagcacaa tgtacaagat tacctgcata tggatgtcta
     4561 tcatgtacca taagttttgt acagtcttca tatataccta ttatcttatc tatattgcta
     4621 aaatctagct ctgggtctat tccttgtgta taaagattaa gtcccacatt tactatatcc
     4681 atattcccag ttctttcacc atttccaaat agtgttcctt caagtctatc ggcaccagcc
     4741 aataatccaa gttcacttgc agccgtacaa gttcctctat cattgtgagt atgcaaactt
     4801 agtataatag aatccctaca acttatattt ttacaaaacc attctatttg gtctgcatat
     4861 acgtttggtg ttgccatttc aactgttgat ggaagattta ttattacttt tttttgagga
     4921 gttggtttcc aaacatctat cactgcctca catatttcaa gtgcataatc catctctgtt
     4981 cctgtaaaac tctctggtga atattcaaaa gtaaattctg tttctggata tttttttact
     5041 tcttcattaa ataattttgc tccttcaact gctatatcta ttatttcttg tttactcata
     5101 ttaaaaacca catctctttg aagaacagat gtagagttat ataaatgtat aattgctttt
     5161 ttacatcccc ttattgattc aaatgttttt tctataagat gagccctaga ttgtgtaagt
     5221 acttgtatag ttacatcatc tggaatcata ttctcatcta ttaattttct taaaaatgta
     5281 tactctgtat ttgatgctga tggaaatcct acctctattt ctttaaatcc tgtatcaata
     5341 agcattttga acattctgac tttttcattt acactcatag gtgttggcag tgactgattt
     5401 ccgtctctta aatcgacact acaccaaatt ggagccttaa atatttcatt atctggccat
     5461 ttacgattta ctatttgcat tttatcatat tttttatact ttccacactt catgttattt
     5521 cccccttttt ttgttttttg tattcagtat acctcatatc gacgcaatcc tgcacaattc
     5581 ataaggataa atgtcaattt tatcattaga ataaaaaaaa cctcatctct aataaaatta
     5641 gagacgaggt tttgctcgcg gtaccactct atttagcttt ctaaaaagct cccttagtaa
     5701 tagccaatac aataattata atgactatat ttcctataac gtggaattac tccgattatt
     5761 ctaagattta aatcttagaa aacagctcct agacaagttc agaactatca actactaaat
     5821 cacaccatcc tttagctctc tgaaagtttc attgttctta ctacttctat tcatcacttt
     5881 tttaatcatt taaattttct aaatattttg atttaatagt tattatatac acattaaaat
     5941 gtattgtcaa cacaaaaaat aattatctca attttttatt tttgataagc aattctataa
     6001 aacatgctaa ctattgaaaa cagtatgata ttatgcctta aactttttca tcttttcatc
     6061 tatgatattc aataattttt tttcatcttc caaactcaat gcccttaatc taattttata
     6121 attagattcg tcagtgtata tatttaatat attttttact acttctattt tttctattat
     6181 cttataatta aacttttgac tttgtttaga ttttatactg aatatacatt gattatttat
     6241 taatattgaa tgattatcga gagtaatata tacttttccc tcatatttat ttgaaaaatc
     6301 acgaccttgt ttaaatagaa aaactgctgt tattatacat aaaatatata atattgtcgt
     6361 tgaatatata aaaattgtag ctaaaattat aagtgctaaa accatatata tagtcattcc
     6421 ttttttaaaa taaccattgt cataataatt catatagata aacttatttt tactcaaatt
     6481 ttttcactcc caagattcta aaaatttata tcaaaaaatt ctataatttc atcttcatga
     6541 taatttatga ctaattcatc tggaaaatct actgcttcta aaactttttc tgcattttca
     6601 aattctccta tagaataaca tatatgagag tcagtaccaa gaattactct tgcaccatat
     6661 gttttacaca atgtcaacat atgacttaca ttttcccaag tacccgttct atgagagttt
     6721 gaacttaatg atgaattatt tatttctaaa aggatatttt tatctttagc tttttttact
     6781 ataggttcat aatctaaagg atacctactg tcatctggat gtccaattat ttttacctta
     6841 ggtttatcca taacttttaa aatagcattt gtattttttt cttttgtacc agaggctata
     6901 caaggtctat gcaaacttgc gattatataa tccaaatgtt tcaacatatc ttcttgaaca
     6961 tctaaattac cattataatc cattatattt ccttctattc ctctaagaat ctttacgcct
     7021 tgcacttcac gtggaagcaa atgcaaatta taaaaataga atggatgtgg tcctcctggc
     7081 atattaggtc catggtctga tattcctaag tattttatat tcttcttact cgcctcttcg
     7141 acattttcct tgaaagtact atgtgcatgt ccacttatta gagtgtgaca atgtacatcc
     7201 ataatagact ttttcattaa atttcctcct aatattttac ataaattcgt gaaaatagct
     7261 aaagggctat ttcaaaacta aagctgtcat aatagacagt gtaacgtttt aaaacagccc
     7321 cattttcaat tattttacac ttctaaatct ttaaatttat ctttaaataa ttctccaata
     7381 cttcctaaag attcctctac ttctaggtat tctgttatat cctcttctgg ctctctagtt
     7441 gcttctttta ttgaaaccaa cattctttta gtctttggtt taaaatcaag aatcttaact
     7501 ttaacctcat ctccaatatt aacaacatta gtaactttag ccactctagt atcactcaat
     7561 tcagatattg gtaaatacgc atctacacct tctttaattt gaacaaaagc ccctttttct
     7621 attattctaa gaactttagc attgacaaca tcatcaattt gtacttcttt tgctattaaa
     7681 tcccaagggt cattatttat gtcttttaat gccaaggcaa tttttttagt ctctttattt
     7741 acatccaaca cataaacttc tacttcttgt ccctctgaaa caacatcttc aacactttca
     7801 actcttgtcc aagctaaatt atttatatgt gctaatcctt caatcccacc aatatccaca
     7861 aatgcaccat aaggcattat ctttgtcacc ttaccatctc ttttttgtcc aacttcaaga
     7921 gaattaaata agtcttcttt agctgctttg atacgagctt tttcttcttc tctttcttgc
     7981 ttaattcttg ctctttcagc ttctttttct gccttaattt gagctcttct ttcttctctc
     8041 tctttattta tacgctcttg taatacatct ctatgagatg ctacaagtct attttttcta
     8101 acatccaatt cttttatata aacttctaag ttaagaccaa caaatttaga tgtatctgta
     8161 ataaattttg tatcaagttg agaaattggt ataaaaaatg tatatgtatt gtaattagca
     8221 aatacacctt tatctatatt cttttcaaca tttacagtta ttattctatg ttcatcataa
     8281 gccttttgta acttggcaaa atcatttctt ttgtcaagtt gtaatttaga taaagttatt
     8341 gtaccatctt tttgagatac ttttgttatt acagcagtta tttcatcacc tatgtggtat
     8401 atatcttcta tatattgatt tttttctatg ttcaattcac ttataggtat tattccatca
     8461 aacccatagt ctaatcctaa ttgtacttca tcatttctca ctgcagttat ttttccagtt
     8521 gtcaattctc caactttcac tttgctgaat acttgttctt gctggtcaag taattcttgc
     8581 attgttaaat cattttccat tataaacatc ctttcctctt attgattatc atatctgtaa
     8641 atattatacc attaatattg caaatactta catttttctt actataattt ttaaaataaa
     8701 ttcaacttca tatatttata cattatagaa aatatcttat taaattaaaa tacaaattct
     8761 aatttttatc taataaaata ttttcataaa aaatttatat tgtatatctt attcttttat
     8821 tgctttaggt tttgatagaa aacatttata aaatataaag aaagaaattg ctgttatagt
     8881 agcagctgtt atatctgcaa ctggctctgc taaaattact gcaaaaagtt tttcattaaa
     8941 gaatgttggg agtataaata ttaaaggtat taaaagtaca atctttctaa gtagagctaa
     9001 aaccaaagat attgttgctt ttccaagtgc taaaaatgtc tgttgacaag ctatttgaat
     9061 cccaaacata aaagcaccag caaaatatat ttttatactc caactagtca tactaagtaa
     9121 ctgcggattc ttattaaata tactaacaaa aaatgcagga aatatcatta ataaaagcca
     9181 taaaatagta gaatatgtaa aacatgacaa caaacacaac ttaaatgtct tttttactct
     9241 atcaagttga cctgaaccat aattataact tattattggt tgtgctcctt gtgtaacccc
     9301 catattagga agcataagaa tttgcattat actgctcatt atagccattg aacctactgc
     9361 taaatctcct ccatacattt gtaatttact atttaaggct ataagtacta aactttctgt
     9421 agattgcatt ataaatggag agattcccaa accaagcact ggtaagatga tacttgcttt
     9481 tggtatcata tacttctttt ttattttaag tatacttttc tttccaaaga gaaacataag
     9541 tacccaaata gcagaaacgc cttgtgcaat tatagtagct aatgcagctc cttttacacc
     9601 catattaaag ccaaatataa aaatcgggtc aagtattatg tttattaaag cacctatagc
     9661 aactgtcatc attccagttg tagcaaaccc ttgggtattt ataaaagtat tcatacccat
     9721 tgaaatctga acaaacactg tacctattaa ataaatattt aaatagtcca ttgaatatcc
     9781 aattgtagat tcacttgctc caaacatcca aagtaaaggc tcttttccaa tcttaaaacc
     9841 tatagtcaaa agtattgcta aaatcaccag tacagaaaaa ctgttactca ttattttctc
     9901 tgcctcatca ttgttttgct cacccatttt tatagcagct aatggacctc ctcccatacc
     9961 gattaaataa ctacatgcag ttattataat tattattgga aatgccactc caacaccagc
    10021 catagcaact tctccatttt ccattctacc aataaaaatc ctatcgacta tgttgtataa
    10081 aacatttaca atttgtgcga taatagcagg tgtagcaagt ttaaataata atttacccac
    10141 actttcacta cctaagttag tgtttgaatt ttccatttta aaacctcctc aatccaataa
    10201 ttaaaatgta tcactaaaat tttattctat ataaaaattt cattttatta gcagacaaat
    10261 ttagagtact ttccctacaa gtaatactat actatatttc ttttgccata gtaaacattt
    10321 tagaactaac tgaagttaaa tactttatct cattttttat ttattattaa aaatttttca
    10381 cacatactca taaaaaaact atccaagtat attacttaga tagttttata ttatatgtaa
    10441 agaaagagct gaaactataa cccctgataa caaattacca agaaatactc ccataaaagc
    10501 atcttttatt ctcattttta atatggaagc tatagcagat gcagtccatg ttcctgtagt
    10561 tggcagtggt atacctacaa atagtattat accaattatc gaaacacttt ttaatttctt
    10621 cattcttgag tttatttttc tatcaattac atcagcaatc cctttaaata gtttcttccc
    10681 tcttaaaaat tgtaatacat gtctgaatgt caatatcaag ggaatcgata ccaatgtaga
    10741 acctattaca ctaaatacat aaacccatat aggatttaag cccattgcaa tacctatagg
    10801 aatggctccc ctcaattctg ttacaggaac catagataaa attaatatgt gtaaatatcc
    10861 caaactattc cctcctattc taagtgacat taagttatga atttatttgc tctataacct
    10921 caccatcctt tttactaact aaaaatgcat ttttagtcaa tgaaagcatt ggtaaatcac
    10981 tcttagagtc tgaatatgca tatgaatttt cataatcaat ctctatccca agtttaccta
    11041 atacagcctt tattcttttt aatttttcaa ttcccttaca attttctcca atgaattgat
    11101 ttttatatct tgaattttca taaaaaaact ccgtgcctat aacctcgtct gcaagaccat
    11161 aatacttaaa atatttcata tatgcatatg gagatgcagt tatcataatt ataacattat
    11221 cttcttcttt atttttgtaa ataacctttt tcatagattc gttaattttt ttcattatgc
    11281 aatcatcaaa aaactttttt aaatcttctt cactaaaatc tactattgga taaaagattg
    11341 cctcttttgc cttcttcaaa ggtatcattc taattaaata cataaataga ataaaaataa
    11401 tgtatggcaa ctttataata gttttaaagg ttttattttt taaagagtaa ataaaaaaat
    11461 ctatcatgct atcagtattt ataacagtat aatctacatc aagtaataat acactttttt
    11521 tcataagtat ttccccatcc tatcactaaa aatatcacta aaaaatcaat attattaaac
    11581 atgtaattat ccataagatt acgtttattt ttaaagcatt atcttttagg acaatatcag
    11641 taggattatt ttcatttgta gtgttaatta tataattata tctaagtaca ccataaacta
    11701 caaatattgt tgtaaatatc atatgtggct tttctggatt acttgtagaa tacaatgcat
    11761 aacatacaat tgtagaactt aatataacta tcatcatttg atttagattt tctatgtcat
    11821 aatcttccaa tatttttcta tgttcaactg catcatctct tagagtttct atttcttttt
    11881 tacgttttcc aagagctaag tacagagata agaaaaatgt acataaaatt atccaatttg
    11941 ataaactaac acttatggct acacatcctg cataaaccct aagtataaaa ccaagagata
    12001 ttgaaaacac atctaatagt attatattct ttagtttaag agaatataat atattgttta
    12061 tcaaatagag actgagaatt atagaaagat taaaactaac aactgttgat aaacagactg
    12121 aaattaaagc taatactaca aatagtatat tagcactttt tttgcttatt cttccacttg
    12181 ccactggtct attacatttt ttaggatgtt ttttatcttt atccatgtct atagcatcat
    12241 tcagtgcata tactgatgat gacattaggc aaaaacacac aaacgcaata aacgtattta
    12301 aaaataaatt tatatttagt atgttgtttg aaaaaattat agctgaaaat acaaagaaat
    12361 tctttatata ttgctttatt ctcattaact ttatataatc tgaaatttta gtaagaagcc
    12421 ttatacttgt attggtctgt ttcataaact tactaccctt tcaatattat tttttaaaca
    12481 ggatatgatt ttatccctat gtttaaaatt tctataaatc cattgacatt actgattact
    12541 atagtaacca ataacagcga tagaaatata ttatacatat tcttttcatt ttttctaagc
    12601 gctttcgtaa agtaagccaa taaaattata actgcaaaac taaaatttat agccataata
    12661 cttgaattga ctagattata tcctactata ccatgtaaag ttagattgaa tataattatt
    12721 cccatacata gcttaggaac tcttttttct gaattttcaa taactgataa aacacaaaaa
    12781 cataaagcta ataatgtaag aattactggt attgtagcaa cttctccatt ttgcaaaaat
    12841 aacccttttt taatgcttgc tccaggaaaa taaaaaaccg aatacaataa tctctctata
    12901 atcattgcaa tcttttccaa aattggttct ccacttataa atttcgtatc atactgtatt
    12961 acatttccaa ggttcataaa actctcataa gacttcgttg aaactacaca aatatatgcc
    13021 attaggataa atgtaattaa atgttttaac atttttccta tttttaattt aaactcaaat
    13081 atcatattaa aaaaatacaa atatatatta ctaatagtta ttccaaatag aggaatagct
    13141 gctaattcta ctaaataaga attttttcct ttcatcttat ttgcaataac tatgaaaaat
    13201 attaatgaaa attggctata tataaatctc tctacattta aagttataaa taactgtgga
    13261 aaagaaaata tcattatcat agtaagtaaa attaaagtta atctctgcat ttttatcttt
    13321 ttaatatgat taaagactaa agttattgac atagcactag ttattatttg tagacaaatt
    13381 agtccaaacc cataggcact attaaaatct gttgtcccag gatttgctat tgaagtacaa
    13441 aataggtata ttggatatgc aaaaattgat ataattgctg aaaacaatat gtgcttacta
    13501 ttgtcttgac ttatagcaaa tacatttaaa ttgaataaaa ctcctgtatc tgtatctaac
    13561 aatacatcat aagtattgtt taaatcataa aatatagtag tcttagaaaa aaatatctta
    13621 gttattatta agtaagttat tacaactaat ataaacaaaa acatctcgtt tttatatttt
    13681 aatattttac tcatcaatat atcaccatcc attcattata taggatttgc tgacacctac
    13741 aatttattcc ttatatttaa tagccatctt cataatgttc ttacttgttt tattgtaatt
    13801 gaataactct ttatttacaa ccacaatatt tactttattg atgttataat cagtcaagta
    13861 ttgtttatat aacatagatt agtgttgtat aaaatgagaa tttgtaagca atattaatgt
    13921 aaaatatttg aaaaacttta tataaaaata tacaatgttg cttatggaat taaactataa
    13981 caaattaaag cataatttaa ggagacaaaa ttataatctg tctccttaaa tattaaaact
    14041 atattttaaa acattactaa atactataca aatttcaaat atagtataaa attaattaga
    14101 ctttaacact attagctcca tcttcttccc attttttaat ttcttttaat ctatctatta
    14161 taggtaaatg ctgagtacat tcaacttcac attttccaca agatatacat tctttagctt
    14221 ttgccttact attcattgtg taattccaat gtcctaagcc ataaactaac tttgtcattt
    14281 cttcatcact ctttttaaac atctgctttt cattataaaa aagcatatag gctggggtat
    14341 taataccttt tggacatact ttacaataac cacaacctgt acatatttcg ttcatattct
    14401 tatttaatct agtctcaata tcatgtatat cctcatctga ataaattcta ttttcgtctg
    14461 caattttaca tgcatcatct atatcttgtt ttttattaaa ccctatcaaa gatatactta
    14521 tttgtgaaat tcctatgttg aatcttaaag ctgattctat agtactttcg ccatcttttg
    14581 atagaaaaga aagttcttta ttatactttg gaatagttcc accacttaat ggattcattg
    14641 ccacaacacc atatccatta tcaacagcat atttggcacc ttttagccta tatggaaagt
    14701 ttaatatatt tattcccata gttacacctt caaacttatt ttcacttaga atatctataa
    14761 cctcatctcc tggttgatga gatgaaaata ctatatggtc tatcaatcct tcttctttac
    14821 atttcaataa tccttcatat tgacctccag gtctcattgc tagttcgtaa tgttccatct
    14881 ttctaatatt ccaaacatga taaaaattta ttttagatac gcctaatcgt tctattgatt
    14941 tttttacagc ctctatagct ttttctgatg tatcacatgc ttttggcttt tctttagttg
    15001 atacataaaa atcggtctta ccttccttta tcatttgtct aaaggctagt ccaaatatat
    15061 cttcacttct gtcatcacaa taccctggtg ctgtatcaaa gtaattaata cctttttcat
    15121 acgcatattt gactaaatca gcattttctt gattactttt ttctaaatca aaccttaatc
    15181 ctccatagcc aactactgaa accattttcc ctgtctttcc atattcaacg tatctcattg
    15241 taaattcctc cttagatata ttaagactta atttctttat agaaaatatt ataccataaa
    15301 taatttttat aaattattta tggtataata ttttttttat aaattatatt tttttataac
    15361 ttgtatttta taactaagtt aaaaactata aaaaggttca atatggacta ttacttgagc
    15421 attttcatta atttcttctt gtatttgttt ctcaatttta tgtgttaaat catgagattg
    15481 ctctacgctt ataaatgggt ctaccattac atgcatgtca atataaatag aacttttgct
    15541 acctctactt ctgatattgt gaacatccct tacctcatta aattcaaaca caatttcttt
    15601 tatataatcc tcatctacag ccactctatc gaccaaaatt ccaatagatg gcctaaatat
    15661 tccataagcc gaataaatta tgaatgcaga aataataata ggaactatag attctattat
    15721 tactggaaat cctaacttaa caccaactaa agtaaccaat acacctagag acacaaatat
    15781 atcacttctt gtatgcaatg aatccgaaat caaaacataa ctatttagtt tagtcccaat
    15841 tctatattca tacatgcata caaaaatatt tataaaaagt gtaataatta aagcaattaa
    15901 actttctata gttatagcag gtacaactgg attagctatt cttaacactg cactaagaat
    15961 tatcttaatt gctataacaa acaacatacc acttataaat aaactggtta taatttcaaa
    16021 ctttttatgt ccataaggat gatttttatc ttttggttta gatgcaaaga aaatacctat
    16081 aagccccact atattagatg ctccatctga caaagaatga aatccgtctg ctgtcatact
    16141 atagcttttt atttgattac caataatgat ttttaataat gctactgcaa aattagcaaa
    16201 taaaattatc cacaatactt gcttcacttt cttatagtta tccataacaa attccccctc
    16261 ccttcgattc aataaagaaa gctatctatc aatatcaatc aaccagacaa taaaatcgta
    16321 tataagcaca tttactatat ttatattcaa aattggttgt acttgttcat tctatttgaa
    16381 acttaattat aaataaaaat cgtaggtatg gttttggtat acataaaaat aagagactgt
    16441 ctcaaaataa agataatctt cttttttatt gttgaaatga taaaatatat ttgatttcat
    16501 ataaaaaaga gtgcctcatg aactaaaaag ttcattttga gacactccct taaatttttg
    16561 atataaacta ttaatttttt atactaagcc ttctttaact aagaatgaat gagcaacttc
    16621 ttctggtgat ttaccaagtt catccacttg atagttaagt tctctcatag tttcttcatt
    16681 tatttttcct gaaagcatat tcataacttt ttctaactca ggatatttct tcaaagtatc
    16741 ttctcttact aatggtgctg cataatatgg tacaaagaaa tttttatcgt cctctaatat
    16801 cttaagatta aactttttaa taagaccatc agttaaaaat gcatctataa cctgagattc
    16861 attattatct aaagctgtat atcttaaact tccattcatt gcttttacat ctttgaattt
    16921 taaaccataa tctctagaaa gtcctacaag accatcttct ctattttcaa actccaaagt
    16981 tggtgagaat gtaaattctt taccatattt agtcaaatca gaaatagtat ttattccata
    17041 cttttcagca gtttcaggca tcatagctaa tgtgtaagta ttattaaatc caagagggtc
    17101 aagtaaagtt aactgatttt ctttttccat agtatctttt acaacattat atgcttcatc
    17161 agcatcttta atcggagcat gtttcattac atttaccaaa agtgtacctg tatagtcaac
    17221 atacatgtct aactcaccag atttaattgc attaaacgca acagatgaac cacctagatt
    17281 taaattcttc ttaactttaa gattcgtatt tttttctact aattcagcat aaacattacc
    17341 aagtataagt tgttctgtat aatctttaga accaatagtt attgtatctt ttttacttga
    17401 aaaactagaa ctaaaaacaa cgaacaacat tgaaacaaca agcactacag atattacttt
    17461 aagtgctata tagttcttct taggtgcttt tggatttaat ccttttggag taactgcaac
    17521 ttcaatttta ctaaataaat aatcaactaa cagtgcaatt atacatgatg ggattgcccc
    17581 tgctaaaatc atattattat ttacagtttg aacccctgaa aatactaaat atcctaaacc
    17641 accagcacct ataaatgctg caagagtcat caaaccaaca gctgtaactg cagatattct
    17701 aactccaccc attattatag gaagtgctaa agggaattgt attttaaata atgtctgatt
    17761 tcttgtaaga ccaatacctt tagctgattc taatactact gggtcaatac ttgttatacc
    17821 tatatacgta ttttttacaa ttggtagtaa tgaataaacc actaccataa atatggctgg
    17881 tttacttcct ataccaagta taggtacaag aagacccaaa agtgccatag acggaactgc
    17941 ttggactaag tttacaaatc caagtacaaa ctttctaaga gatgagattc ttgaaataat
    18001 tattccaaga ggaacaccaa ctattatagc tattaaaata gatgtaacag ttagactcat
    18061 atgttgtatt aaaagctcta taatcttatc tttttgtaat agtataaagt taaaaaaatt
    18121 agccaagaac actcacctcc atttctaaga attgttcact taaaacagaa agtaaactac
    18181 ttctagtaat aagccctacc agtttatttt catcagaaat aaccggaata tatccaaccg
    18241 aattacgatt cattacattt agtatttcta ctaaattatc accttcattt acatgcaaag
    18301 gctcacttga cataatatca gcaagtaata ttgacttatc atcaatatct ttcatatctt
    18361 ttacagtaac aatacctttt aatgtttttg ctctatctat aattagtaag ctatcaacct
    18421 tactcgttct cataatttct attccttgag ttacagttct agtagaatta acagcaatag
    18481 gattttttat cataatatct cttgctttta tatattctgg attatcccaa actctatctt
    18541 ctccgataaa atcttttaca aagtcatttg caggctttct aagtatattt tcgggagtat
    18601 catattgtgc tattcttcca tccttcataa tacaaatttt atcagctatc tttaaagcct
    18661 catccatatc atgagttaca aatattatag tttttttcaa ttcatcttga agagaaaata
    18721 attgttcttg taaagatgtt ctcgttattg ggtctaaagc actaaaaggc tcatccatca
    18781 atattatttc agcatcagta gcaatggctc ttgcaacacc gattctttgt tgctgtccac
    18841 cactaagttc tgaaggatac ttattcaaaa attcatcact atcaagtcct accatctcaa
    18901 gtagttgcaa cgttcttttt tcaatttctt caacggattt ttcctttttt aggcgtggaa
    18961 ttagctctat attttctttt attgtaagat gagggaatag gcctgtgttc tgtataacat
    19021 atcctatttt tcttctaagc tctatttcat tttcctgaga aattggtttg ccattaatat
    19081 atatctcacc agatgtaggt tttattaatt tgtttataag cttcaaagta gtagtctttc
    19141 cacaaccact tgaaccaatc aaaactacca atgtacctgt ttctacaact agagaaatat
    19201 catctaatat aacattattt cctatttttt ttgttacatt tcttatctct atcataattt
    19261 atcatcccct ttttcgaaca agcaaccttc ttcaatatta taagttgttt ttataattat
    19321 atcatactat tttatttatg caaacaaagt acgattttta ggtcactatt tgtctgaata
    19381 ttgctac
//
LOCUS       DAMJYC010000075        19088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJYC010000075 DAMJYC010000000
VERSION     DAMJYC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.076"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..1356)
                     /locus_tag="PC472_002806"
     CDS             complement(<1..1356)
                     /locus_tag="PC472_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2728121.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECPPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGEIGPTGVTGPTGNTGATGSIGPTGVTGPTGNTGVTGSIGPTGVTGPTGNTGVTGS
                     IGPTGATGPTGNTGEIGPTGATGPTGVTGSIGPTGSIGPTGVTGPTGVTGPTGSIGPT
                     GEIGPTGATGPTGATGPTGPTGVTGSIGPTGATGPTGNTGVTGEIGPTGVTGPTGATG
                     PTGATGSIGPTGVTGPTGATGVTGEIGPTGPTGVTGSIGPMGVTGPTGEIGPTGATGP
                     TG"
     gene            complement(2034..2558)
                     /locus_tag="PC472_002807"
     CDS             complement(2034..2558)
                     /locus_tag="PC472_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2728122.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(2623..3375)
                     /locus_tag="PC472_002808"
     CDS             complement(2623..3375)
                     /locus_tag="PC472_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2728123.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(3406..4704)
                     /locus_tag="PC472_002809"
     CDS             complement(3406..4704)
                     /locus_tag="PC472_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2728124.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYITKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(4721..6985)
                     /locus_tag="PC472_002810"
     CDS             complement(4721..6985)
                     /locus_tag="PC472_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2728125.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(7240..8058)
                     /locus_tag="PC472_002811"
     CDS             complement(7240..8058)
                     /locus_tag="PC472_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2728126.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(8051..8749)
                     /gene="cbiQ"
                     /locus_tag="PC472_002812"
     CDS             complement(8051..8749)
                     /gene="cbiQ"
                     /locus_tag="PC472_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2728127.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(8752..9030)
                     /locus_tag="PC472_002813"
     CDS             complement(8752..9030)
                     /locus_tag="PC472_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2728128.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(9020..9772)
                     /locus_tag="PC472_002814"
     CDS             complement(9020..9772)
                     /locus_tag="PC472_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2728129.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(9954..10146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10278..12050)
                     /locus_tag="PC472_002815"
     CDS             complement(10278..12050)
                     /locus_tag="PC472_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2728130.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(12064..12993)
                     /locus_tag="PC472_002816"
     CDS             complement(12064..12993)
                     /locus_tag="PC472_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2728131.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            13222..13410
                     /locus_tag="PC472_002817"
     CDS             13222..13410
                     /locus_tag="PC472_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728132.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            13403..14104
                     /locus_tag="PC472_002818"
     CDS             13403..14104
                     /locus_tag="PC472_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728133.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            14106..15029
                     /locus_tag="PC472_002819"
     CDS             14106..15029
                     /locus_tag="PC472_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728134.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            15186..16109
                     /locus_tag="PC472_002820"
     CDS             15186..16109
                     /locus_tag="PC472_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728135.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            16102..16839
                     /locus_tag="PC472_002821"
     CDS             16102..16839
                     /locus_tag="PC472_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728136.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            16850..17623
                     /locus_tag="PC472_002822"
     CDS             16850..17623
                     /locus_tag="PC472_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728137.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(18164..18772)
                     /locus_tag="PC472_002823"
     CDS             complement(18164..18772)
                     /locus_tag="PC472_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2728138.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(18950..19066)
                     /gene="rrf"
                     /locus_tag="PC472_002824"
     rRNA            complement(18950..19066)
                     /gene="rrf"
                     /locus_tag="PC472_002824"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6007 a         3379 c         2435 g         7267 t
ORIGIN      
        1 tcctgttggt cctgttgctc ccgttggtcc tatttctcct gttgggcctg ttactcccat
       61 tggtcctata cttcctgtca ctcctgttgg gcctgttggt cctatttctc ctgtcactcc
      121 tgtcgctcct gttgggcctg ttactcccgt tggtcctata cttcctgttg ctcctgttgg
      181 gcctgttgct cccgttgggc ctgttactcc cgttgggcct atttctcctg tcactcctgt
      241 atttcctgtc ggtcctgttg ctcccgttgg ccctatactt cctgtcactc ctgttgggcc
      301 tgttggaccg gttgctcccg ttgggcctgt tgctcctgtt ggccctattt ctcccgttgg
      361 tcctatactt ccggttgggc ctgttactcc tgttggacct gttactcctg ttgggcctat
      421 acttcctgtt gggcctatac ttcctgtcac tcctgttgga cctgttgctc ccgttggtcc
      481 tatttctcct gtatttcctg ttggacctgt tgctcctgtt ggtcctatac ttcctgtcac
      541 tcccgtattt cctgtcggac ctgttactcc tgttggtcct atacttcctg tcactcccgt
      601 atttcctgtc ggacctgtta ctcctgttgg tcctatactt cctgtcgctc ctgtatttcc
      661 tgttggtcct gttactcctg ttggccctat ttctcctgtc gctcctgtac ttccggttgg
      721 gcctgttact cccgttggtc ctgtacttcc tgtcgctcct cttggtccca ttggacctgt
      781 aattctaatt ggaggacact cacatttagg ataacaatta tctataattc tatcttcaaa
      841 tgttacaagt atatacggtt tgttacaacc tctactagat gtaaattcaa gaatctgata
      901 agggtaattt aaagataaag ctaacccata attaggatat ttattcattg cccatgcttt
      961 tatagttccc gtaatattta aacaaatata gttggatgta tcacttatcc caattggtaa
     1021 atatccccta atattttcca tagacactct atcattccat gttatagttc gtaaattata
     1081 atattcactt attcttttaa tttcaatatc tactttttct ttaaaaatat gattcttatt
     1141 tgaatcaata tgaataaata actctgcttt taatatactt ttgtctttta atatatctgg
     1201 tattctcact ttaaaaaaag taatcaactt ttcttttaca aaactatttg aacatttact
     1261 tcctattaag caataatcta aattactaaa gtttttatct ggatattttt tagatataaa
     1321 agtatcatca tttaaataaa gtataatatt tctcatttat aatccctcca ttttgggtct
     1381 atatcatact atgtttaaaa tgcatgctta gttacaatac aattaaaatt tatagtatta
     1441 aatttttata taataatgct gatttctaca aaaaaagaac atgtcattag aaatttctaa
     1501 tagcatgttc tttttcatta ttatatatta cactttaaat tattccttga ctggcttagc
     1561 tggtgtaaac taaaccagtc tttctaaaat attaagcact tttttcttat tatgtttttt
     1621 aggatttatt gtctatatta ttgtaaacca aagttataaa attgtaaata gaatatatta
     1681 ccataacaat atttttttat aagtaaaaat tataaaaaac tttatttttg ttaaatagaa
     1741 taaataaaat aagtttaaaa aatactgaaa taagtgtttt ccacatattt tatatattta
     1801 tttaatacat tattatttaa aaataaatat ataaacaatc ataataaaat ttttttaata
     1861 aaactttatt ttttagttaa aatcatacaa tatatagatt tccacaatac aaataaatta
     1921 aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct catttttaag
     1981 taatatatat ttttaatttt tatttataac taaatctagt ttttatataa attttagttt
     2041 ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt cataatttgt
     2101 ggagtttgtg gcttgtcagc tgaatctgtt ctaactgatg ctatttcatc aactgtatcc
     2161 ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg tgagttttta
     2221 tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc cattgatatg
     2281 actcctcttt catgttttaa attgttagta aatccattac cactaaattc accttttatt
     2341 gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat cataaatcct
     2401 ggtattactc tatgaaatat tattccattg taatatcctt catttactaa tgatacaaag
     2461 tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc ttttccattt
     2521 tccatttcta tagttactat aggattttta ttttccataa tttattacct tccttttcat
     2581 tagttaaatt acataattac atttattata ttatactcta aattaaagtc tatcaagtat
     2641 attttttcct atttcatatc cttcttgata aatagtttta caaaactcat catcaaagtt
     2701 aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt tatcttcatc
     2761 atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata ttaaaataat
     2821 tttatccaca ttatccccaa ttaatggttc aattaatgca cttctaatta aacctccatc
     2881 taatttaaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt ctttaaatcc
     2941 aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga taatggcttc
     3001 attacgaggc atttttgata cgtcaacaat cttttctgat actactttat tatcaacttc
     3061 aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt cccttaggtt
     3121 gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc cattctggat
     3181 atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt acaaataata
     3241 tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta ttcctccatc
     3301 atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac cttctaaaca
     3361 taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata tttcaaaaaa
     3421 cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt ctcttataga
     3481 atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat gagtagaaga
     3541 tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt cttgatactc
     3601 aacattagct tctctacata tatttttata aatagctata gaatatccat cactagtata
     3661 tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt tattagttgg
     3721 gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg atatcataaa
     3781 agatgaataa atagactcaa agaatccctc tctatcctta cccattgata tgcaaattct
     3841 ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc taccaacttc
     3901 ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat taacaagagc
     3961 attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata taaattcatc
     4021 attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa aatctataat
     4081 atcttccata tttacatcta agttagatgc tatttgcttt ataagaaaat tatctttttc
     4141 taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat tatatgagtg
     4201 accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac aaattggctt
     4261 tttaaagtct ataagccttt catttggttt taatatacta tcacctttta aaactactct
     4321 accagctatc ccaagaggtc tatctaacca tgtacttaat attgctcctc catagcattc
     4381 tgtatttaac ttcaaataag atttttcagc ttctatttct gcattcggtt taatactaaa
     4441 tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta taccttctga
     4501 atttactaca aaagctacta tagctgataa attcttagta atataatact ttccaccaac
     4561 tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat ctaagatttc
     4621 cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt ctattaagtt
     4681 tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata tttagtgttg
     4741 tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta acatctgtac
     4801 caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta gaaccaagtt
     4861 ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta actttatctg
     4921 caactttaga tttgcttata gtactcatat actttttagt atatttatct aacatattat
     4981 aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct atacattctt
     5041 ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca tataatgttc
     5101 tcttctctgt caatgacata tatagctctt ctttagctct agttatacca acataacaaa
     5161 gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt gatataggaa
     5221 ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct ttagatgtat
     5281 gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca gatgtaagtg
     5341 ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca ttactacttt
     5401 caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct tgagcttctt
     5461 cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc tcgtccatat
     5521 atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact tcttttatag
     5581 ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc ttagtagata
     5641 tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt tttaagttag
     5701 ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga acatttataa
     5761 tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac gccaacaaat
     5821 cctttatttc ttttctctca tagaattttg taccaccata tatattgtat ggaatttgac
     5881 ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa agtactgcaa
     5941 aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca gcaatgaaat
     6001 cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct tctttttttt
     6061 cactccaaag cttttttctt tttctttcta tattattaga tatgacttta ttagctgcat
     6121 ctaatatcac ttgtgtagac ctataatttt gttctaactt tacaacatgt acattgtcat
     6181 aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca taaatacttt
     6241 ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat tttatcaatt
     6301 catattgagc tttacttgta tcttggtact catctaccat tatgtatcta aatctacttc
     6361 tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta agtattaaat
     6421 catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca taaatatctg
     6481 caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt ggagtataaa
     6541 gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac actttatcac
     6601 ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct gaatcataaa
     6661 ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata cgaacacagc
     6721 aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt tgttcaactc
     6781 tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata tttggtgctt
     6841 gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta gttttacctg
     6901 aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct tctctttgag
     6961 caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata aatatatttt
     7021 aatctaaaat atgaattact tcaaattata attatgtatt atattaatag attatttcca
     7081 atatattaca attaaataac atttttccat ataatttatt tcattgttta ataaaaaaaa
     7141 taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta agggttatta
     7201 tatataaatc tattttttta aaataggttt agggcttaat tatttatttg tattaagttc
     7261 tctttccttc caatacttat acaaatcttc ttctttctta aataagggta aattcatatt
     7321 tctatgtact tttactaacc atggggattc taggccagct tgtataatat tattttcatt
     7381 tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca atacatatat
     7441 gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta tttttatatt
     7501 atttttattt aatcccttta ttatatctac aatagaccta gtacttactg ggtctaaacc
     7561 agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg atgctattgc
     7621 cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa aatctattcc
     7681 attaacagct actagagctt tgtttattct ctctttaatt gttttttcat ccataccaat
     7741 gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt gtttttctgg
     7801 gtcttggaag acaataccta cattttttct aaggttgtat aaacttctct tatcatattt
     7861 taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac ccattaaatt
     7921 cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa catttccctt
     7981 tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt gatacgttaa
     8041 attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca tacaatttta
     8101 tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac agcatacttc
     8161 caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat cttagctctt
     8221 gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta tatattaaca
     8281 ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat attaattgat
     8341 taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc gctctaaata
     8401 acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa ctaaaaaatt
     8461 caaaactata taataaatct gctttattaa aagatatatt tacaagagtt attcctatac
     8521 taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt aaatctattc
     8581 cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa ataaatatat
     8641 tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt ttgtttggat
     8701 ttactttact caatctattt gtatatgcat atttatcaat tataagcatg attaacttct
     8761 ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag ctgcttgtgc
     8821 agagaataat aaactttcta tttctccact tggtggctca tatggactgc taaaccatgg
     8881 tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg ctcctccata
     8941 ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta ataaaagtaa
     9001 taatatgtta gtttttgttt tagcactcat tagctttaac tcctttatca ttattttcta
     9061 gtatgctttt ttctccgttt tctgtaatta aattatatac aattacagta agtatacctt
     9121 ctgctattgc tattggtatt tgagtcataa agaaaattcc taaaaattta attgctgaag
     9181 ccattacccc acctgatggg tctgggaaag ccagtgctaa ttgtattgaa gttattgtat
     9241 atgtggctaa gtctcctatt gctgctgcaa gaaatactgg aatagcatta tttccatctt
     9301 ttttctttaa agccttaaat attaggaatg atacgattgg ccctattatc gccattgaaa
     9361 aagcattagc tcctaaagta gttattcctc catgtgctaa taacaatgct tggaatatta
     9421 aaactatagt tccaagaaca aacattacgc ttggtccaaa cattattgct ccaagaccta
     9481 caccagtagg atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct gataatacaa
     9541 acacaaatgc tccacataaa gctaataata ctttcttttt agggtcttgc tttactattt
     9601 tacctattga ttttaatccg aataagaaaa atggtataaa tataactccc catgctatag
     9661 accattttac tggtaagtaa ccttccatta tatgcattgc atttgatgta actggagtta
     9721 aaatgattaa cataagtgca gctataaccc ctaatttaat attctgtttc atcttagtat
     9781 ccctctctcc ctttctaata attaaaacat aataatacat gtctgattta tttaataaaa
     9841 taaaaaagcc taactcgatg gttaggcttc taattttcca caatctaaat acaaacacat
     9901 aaaacaaaca tatctgtata attattataa gaaactttct ccatccccga agaaattact
     9961 ttaatatttt aggcaggtct cctgacttag cttcatccta ctcctcttcc ttcccatgag
    10021 atataaaaaa tatcatcata gtggctaaca aaagtttcgt ctgcatcaca gtagcggggg
    10081 ctgtaaagga atttaacctt tttccctatt aatcttgctt aaagaaccta taatattata
    10141 tatttaattt ttaaattctt cttaaattat tttctatata aatcattgta taccttttct
    10201 gtgaaaattt ctacattttt tataaatttt attcattttt atatttttat gtatataaat
    10261 tcttttttgt aagtttatta atttctaatt atcttcttta aatacattga aactacatca
    10321 gaaaatctag ttatatctct tgaatatata aaatcatttc catatattaa tctttcagct
    10381 tcaagttctg attcttttcc tgtaaatact cctaatacta atatattttg ctttctagct
    10441 tttctcactt cattggctgt atctctcaaa ccaactatac ccctataagc cttttctcct
    10501 cttaatgttc tctcactact ttttcctatc tttacatcat ttggtcttcc atcgctgaga
    10561 actatcagga ttttattttc ttcatctcta tttattagac cactacatat agattttatt
    10621 gccaaaccat ctctattatt accagcacaa aaatattcaa atatattttc actatttttt
    10681 atactatcat cataatcttt aaatcttttt aatattgtat agtctaaaaa actagaaaat
    10741 cccatcactc tatttggtat gccaacttct gttagagcat ttgcaattat gtaagcttga
    10801 attgccacat ttccctgatt tctactttga gagccacttg catccaataa tatatcaata
    10861 acgtatttcc ctttttcatt ttctatatct ttataaaata ttttattatt attacttcta
    10921 ccaactctcc aagctctatt tgctaatata gtaccacaat cagaatatac tctactttta
    10981 gcattttctt ctattaaaat tcttgataga ctttctttta atctctttat ttgctgccta
    11041 tacattctag catgttctct aaattttcct aaattatttt ctttttgtct tgttacatat
    11101 tttagttgaa atacattatt acactcactt ctaagaactc catctgtaaa gtgtattcta
    11161 caaccttcat gaacatttct acaattttgt gtctcaattt ttttaatttc acctttactc
    11221 aaataagttt ttccataata atgttctatt tttgaatata tcttatttgc agtgtcttca
    11281 tcaacatata taactcggtc tgctgaattg ctacatttag aatcatcaaa atcccctaca
    11341 ccttctacaa gcatggatga agatattgaa tctatatcac tttcaataat attttcttct
    11401 tcatcactat aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt tacttctaca
    11461 ctttctacat tattttcatc aataagacta ttttcttctc catttaatat gtattcaaaa
    11521 taagttaaat atataacatc taatgctttt aaaatctcca taatatcttg attggtatct
    11581 atatttctta tatcattaac tattcttcta gtcatactat ctaatgctgg gtgcttgtcc
    11641 atactataaa gtacaaatgt gtatttaatt ttatccataa tattatcatt gtgtattttg
    11701 gtaaaacgtg aaacaatatc tttataagca tttcttctta tgtcttctat ccctggccta
    11761 tctttaatga ccttttcttc aactattgta tttaatataa cttgaattag acccagtata
    11821 atatctttgt tgtatccttt tcttattcta tttgtaagat attttttaac tatatcccaa
    11881 tctacatact ttcttctagc acctgcaatt attgcaaaat atagacctat ttcttttgaa
    11941 gagtaatctt tttctgatat atctatactt tcattgtaat caccagctat tgtccatgat
    12001 aagttattag ctctattttc aaattcataa tcatcatata tccatttcat aatctcacct
    12061 atattaaata tgacttattg gaaacacatc tatactctcc catttattag gtattctagt
    12121 tttaattaca tctccaacca tttctttctc atatacatca aaagtctttc ctgtaagtcc
    12181 catattaatt gcaagagttg gttttaatcc tcttcttata gtcttaagag atgccataag
    12241 accacgtaaa tcaatagctt tactagatat ctctccattt tgagacttca attgtaaatc
    12301 taaaaatatt cctgcaaagt gtattagttt ttcttcatca gcatctgaaa attcattttt
    12361 taaaatcatc attaacttat cttcttctat aggaggaata tctattacca taaatctaga
    12421 taccagtgct tcattaagtt cttttgttcc tgcatattca taattcatag tcccaataaa
    12481 tcttgttgct ggatgtagat tgactctctc atacccaggt acatcaatta ttcttctata
    12541 gtctaaagct gaatgtacca caactatagc atcatttttt gccatattaa tttcatcaaa
    12601 caccccaaaa cctccattaa tagcacactc ataaactgag cctcttctaa gtttaacttc
    12661 attgtctata aatgtatctg tacctataag tgttgaactg tctgtattta tatgaaatga
    12721 tgtattccac tgaggtctat taaatatttc ccctagatta tcagcaagta agttttttcc
    12781 cgttgccttt ggtcccgata ataatatatt ttcttcttct aaaatagctg ctatacacat
    12841 agacaagata tctttaccat aaaaataagt tttagacttt gttactctgt attctaaatc
    12901 tttttctaat ccataataat ttctaaaata cagtacatct tcaataagtt tttcatctac
    12961 cccttgcacc cttagattat ctattatatc catattttta ccccttttat ataaatagta
    13021 tttgtttcat ttcttatcac cattttagca taaaaggata taagctaaaa ttgatttttt
    13081 aatctccccc attttaagta aaaccatgca ccattttatc actacaaaat taagagtact
    13141 acttactgat atttcttgat tttattcgtc tatacgtata taatatatac tgataaatta
    13201 tgaggaaagg atgattgaaa tatggaatat atgtcttgcc cagaagtagc aaaaaaatgg
    13261 ggtatttctg aaagaagagt acaaaaacta tgtgaagaaa gtcgcatacc aggagtttca
    13321 aaactcgggt atatgtggct gataccaaag aacgcagaaa agcctgttga tggtagaaca
    13381 aaaagaagaa aggaagtaca atatgaatag cgttttgatt atagatgatg acaaagaact
    13441 ttgtgctctt atgaaaaaat gtgttgagca agaaaattta tctgctatta ctgcacatgg
    13501 tggtgcagaa ggtctacatt tagtcgataa aaatagacac acactctctc ttgtaatttt
    13561 ggacataatg atgccagata tagacggttt tcaagttctg aaaaaaatta gagaaacaag
    13621 taatattcca gtaattatgc ttactgctaa aaatgacgag gatgataaag tttccggact
    13681 acgtttaggt gctgacgatt acctaacaaa accgttcagc ataaacgaac ttatggctcg
    13741 tgtcaattct cttattcgtc ggtatactat gctaaaccct gcttttataa cagacacaga
    13801 tttcataata ataaatggca tgattattga caagacaaac cgaattgttt ctgtcaataa
    13861 tattcctgtt caacttacaa gcaaagaatt tgatttactt gcttttctgg cttctaataa
    13921 gggacgggtg tttaccaaaa aacagattta tacacaagta tgggaagaag aatatgcttt
    13981 tgacgatagt aacatcatgt ctttcatcag taaattgaga aaaaagattg aaccagaccc
    14041 caaccatcca ttttatattt tgacagttcg tggtgtcggt tatcgtttta ataaggaggc
    14101 ttaacatgaa tacaagtgtc tatctgcttt tgatattggc tcttagcctg attattatag
    14161 cgtaccttgt ttctgtcctg ctacgtgtcc gaatacaact tacacttata aaagatgctt
    14221 tagaggatat aaaaaatgga aaccttaatc gacgtatttt aacagatgaa aacgacatta
    14281 caaaacaaat ttgctatgat attaacgaaa tagcaatcaa tagccaattt caacttattc
    14341 agcaaaaaca atcagaacaa gcctacaaac agctaatgac aagtctctcc catgatgtaa
    14401 aaactccact tgcttctttg gtcggttatt tggaagcagt agaaagtaaa attgttgtag
    14461 gagaggaaaa agacgagtat atccatgttg cgtctaataa ggctcattac ttaaagaatt
    14521 ttgtagaaaa tctatttgaa tgggtaaaat tggattctaa ggaacaagtt tttcattttg
    14581 atatttttga cttgaatgaa ttatcccgta atattatatc tgattggata cctgttttag
    14641 aaagcagtca ttttgaatat gaatttgata taccagaaat agagtacttc ttacgtatag
    14701 acgcaaatgc atatacccgt atcattaata acctattaca aaatattatt acccatagta
    14761 gtggcaacaa aatgacattg agaatttttg aaaacaaaga gcaagctcaa attataataa
    14821 cagataatgg gaaaggtata tcttcagaca atctcccaca tatttttgaa aggttgtacc
    14881 aatgcgacca ttcacgagct tcaaagggaa atggtttagg cttggcgatt gcaaaggaac
    14941 ttattaacgc tcataaggga aatattaccg ccaacagcac tcccggtatg ggaacagaat
    15001 ttacaatatt gcttcctaaa gccctgtaaa agtacgaggc tttcttactc taaaacaaga
    15061 taaaagcaag attatggcaa ggttttggca aggttcatgt tttataattc taattgtagt
    15121 tgattttgac gttgtatcaa ctatcttttg tagaaacaaa tttattaaaa gtgaggaaat
    15181 tatatatgaa tgattttgtg attgaaacaa agcaacttac aaaaatttat gggaatcaaa
    15241 cagtagttga caaagtcaat ctccatgtaa aaaaaggccg aatatatggt ctgttaggac
    15301 gcaacggagc tgggaaaacc acaattatga aaatgatttt aggattgact tcaatcacgt
    15361 atggcgaggt agatgtattt ggagagaata ttaagggacg tgaaaaacaa gtatatcccc
    15421 gtatcggtgc aattatcgaa acgccaggat tttatcctaa tctgacagga acggagaact
    15481 tggagatttt tgcaaaactg cgtggtacag ccgcccctaa ttctgtaaaa agagcattgg
    15541 aaattgtagg cttaccttat aaaaataaaa agctgtttag taaatattca cttggcatga
    15601 aacagcgact tggtattgcc aacgctatcc tgcatgaccc agaactgtta gttttagatg
    15661 agccaaccaa tggtcttgac cctattggta ttgcggagat gagaaagttt attagggact
    15721 tgagcgttga gcatggaaag acaattctta tttccagtca tattctttca gaaatctcat
    15781 tgttggctga tgatattgga attatcgacc acggtgttct tttggaagaa agtagtatga
    15841 gtgaactaga aaagaaaaac agtaaatata tcctgctaca agtttcagat attccgaaga
    15901 catcacttat tttagagcgt caatttaaca cgaaggatta ttccgttcaa gatgactaca
    15961 cgttacacct ttataacact aatctggata tgggtgaaat caataaagca cttgtaatgc
    16021 agaatgtaac tgttattagt tctcaacttt gtaatgatac tttagaggac tactttaaaa
    16081 aaatcacagg aggagagggc attgcttaaa cttattcaaa cagaatttct taaactgcgt
    16141 cgtaggaaac ttatttggct gatgttgttg tctgctctca ttatgccgtt tttcgcacta
    16201 ctatacttca attattttgg taaaacaggt gttgagccaa taaagtttta taaatggtct
    16261 gcatttggtt atacactttg gattattttg cctgttgttt tgggtatatt ttgcaccatg
    16321 cttatacacg aagaaaacca gtatgatatg ctcaaacagc tttggattgt acctattagt
    16381 aaaatgggat acttttttag taaatttttc gttgttttga tttactctat ttgttttatg
    16441 ctgattacag ccgttgcttc tgtgttattt agtgtacttt ctggatatgt cgtatttagc
    16501 tgggcaagca ttttatattt gttgaaaaaa tgtttagaaa ttggtgtaat cactgctttt
    16561 gtaatgttgc cgattttggc aatcgctaca tctcaaaaag gatacattct cccaatttgt
    16621 atcacattgg tatatgcctt ttccggtttt gttataatga caataaatat gtatttacac
    16681 ccattatcca gtatgggagt tattatcatg agaaataaag acattccagg tcttatattt
    16741 tcacaggaaa ttaatgtccc attagcattt ctttgtattt gtatttggag tattgtatca
    16801 gtattgtttg caaatattgc actaggaaga agaaagtgag gtgtagataa tgtggaaatt
    16861 actttgggct gaatgtcaaa aaattcgtcg ttcaaaaatt gtttggatta cagtttttgc
    16921 atccattatg atagcagtca tagtttttgc aggggggcag gatgtatata atgacccaga
    16981 tttacattat ggcttaaaga gcgtttatga cggttcacgg tatatagaca atgcaggttg
    17041 gtatatggac gcagtacaac cttggtcaac tttttttgtt cttcctgctg tcattgccct
    17101 attaggaagc tatattattt gtcgtgaaga agaggaggac actattaaat ccctgcgtct
    17161 tattcccata aatgaaacaa atttaaccat agcaaaaatg attattactt ttatattcag
    17221 tatacttctt tatttgttac tctttactat aacatttttg actgaatccg ttttacattt
    17281 ttccgattta tcgacaaaat tggttttaag ttgcatgaaa gagtattttc ttgatggcat
    17341 aggtgtattc tttgcaattt ctcccattat tgccttggta tcgcgcatga aaaaaggata
    17401 ttggcttgca ttagtattca cagaagttta ttctattgct ggactatttg cgggtatgtc
    17461 aaatgtctta caaacctttt ttcctattgc ggcaatattt aatatttctg gatactatat
    17521 aactactgga gaaaaaatta tggggagtat cataagttta ttactttgtg cctgcctttc
    17581 tgcatttata cttaaagggc taaaacacaa tgaaaaaaat tgaaataggt atatcaaaat
    17641 gaaaaagata aaaaaactga ttattctaat tgtattcttg tttctttttg gcatgttaca
    17701 ctgttatact gaatggaaat tcaacaataa cccacctaaa ggggtaatag aaagaataaa
    17761 tgagtggact gataagattg gactggatta gtaacttatg ttaattatag tgaatgtaat
    17821 attagcaagt tattgtttcg tattttaata ctataacttt aaatatttgg ttttaatctt
    17881 ccaaacgaat ataaaaaaac tgtcacagag gcaacatatt tttgaatatt tataaagtgg
    17941 tagttttgat tttcaaagct accgcctttt cttctatata agcaaactta tcctcaacaa
    18001 agtgtaaaac atggtacatt ttaagaaaaa agtaccacag acacaagcaa taaaagaaac
    18061 gccgagaacc tacaaaaacg tagtgcttat gcagatttac gacgccttct attttctcaa
    18121 ccgtcttaat gaccttgtac aaaagaaatt tttctaatat tttttataat cttatcttgt
    18181 gatgtttttt atgtataaat gacttaataa caaatccaat aaaaattatg gatattggta
    18241 taattagaac gaggaattta taatcattat aatgtgacat tattcttacc caactctcag
    18301 aaaaaatata tcctatactg attaatgaag tattccatat ggttattccc acgatagatg
    18361 atattacaaa agttataata ttttgccctg ctatacctgc tataaaagat atgtatgttc
    18421 tacataaagg gattaatcta catacacaaa cagaaataga agagtattta ttaaaataat
    18481 cttgagatgc aaatattcct ttctttgcct ttggacatat ctctattatt ttatttatga
    18541 tagatttacc tccccaattt cccactgtat aacaaaatat cgaaccaagt atacctgcaa
    18601 ttatacttaa aattaaaatt gtcagaaatc caaaatgatt tcttgcagca attgctccac
    18661 ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc atactcaatc ataattaata
    18721 caaatacaga gattaatcca tattgtaaaa taaaactatt gattacagac acttacttat
    18781 ctatctcctt tcttatttaa aggttttaag ggtacatgtt ctaaggattc tcaccctata
    18841 tttaaagaat caatctcttt aaactattat atgtatttac acaaagcatt tataacatac
    18901 tttaaaaata gtaccttaaa ataagaaact taataaataa aaaaagatta cgtggctacg
    18961 tcctactctc ccaggcagtt tcccaccaag taccatcagc gctagagagc ttaacttctg
    19021 tgttcggaat gggaacaggt gtatcctctc tgctattgta accacataat ctttatgctt
    19081 aatatttc
//
LOCUS       DAMJYC010000076        19027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJYC010000076 DAMJYC010000000
VERSION     DAMJYC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.077"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            629..802
                     /locus_tag="PC472_002825"
     CDS             629..802
                     /locus_tag="PC472_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728139.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            802..1413
                     /locus_tag="PC472_002826"
     CDS             802..1413
                     /locus_tag="PC472_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2728140.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            1490..1678
                     /locus_tag="PC472_002827"
     CDS             1490..1678
                     /locus_tag="PC472_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728141.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(1658..1975)
                     /locus_tag="PC472_002828"
     CDS             complement(1658..1975)
                     /locus_tag="PC472_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728142.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            2099..2326
                     /locus_tag="PC472_002829"
     CDS             2099..2326
                     /locus_tag="PC472_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728143.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            2396..3088
                     /locus_tag="PC472_002830"
     CDS             2396..3088
                     /locus_tag="PC472_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728144.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            3066..4319
                     /locus_tag="PC472_002831"
     CDS             3066..4319
                     /locus_tag="PC472_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2728145.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIITIANSKGLEKSLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSIKAQIDYLKQLRIHVHPSCINTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            4325..5776
                     /locus_tag="PC472_002832"
     CDS             4325..5776
                     /locus_tag="PC472_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2728146.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQKEESIKEYEDAFPTKKKTEEGEPLDE"
     gene            5769..6809
                     /locus_tag="PC472_002833"
     CDS             5769..6809
                     /locus_tag="PC472_002833"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2728147.1"
                     /translation="MNNIDYWIKTFNQLEEDVHNKGDNFNKELEKQYDIVLYNILREI
                     NNWYMKFAKDNKISMQEAEKLLNLRELAELKLSLEEYIKYGEENVISQKWMKELESAV
                     KRVRISRLKALELKIRHQVEVLYSKEYEDVNTLIADTYQDSYYHTAFETQKGIGIGLM
                     IAMLSMNNINKIVSSPWTTDGITFGNRIWNKHRPRLMSELNKGLKETLINGISPENLT
                     NKINKNFNTSKEEAKLLVKSELAFFSSLSQRDCFNSLGVEKYEIVSALDSKVCEKRCS
                     PLDGKVIEMKYYEIGVTAPPFHPRCRCVIVPYFDGEESYRAAREEDGGTYYVPSSIKY
                     KEWHKKYVKNTY"
     gene            7119..7379
                     /locus_tag="PC472_002834"
                     /pseudo
     CDS             7119..7379
                     /locus_tag="PC472_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7626..7898
                     /locus_tag="PC472_002835"
     CDS             7626..7898
                     /locus_tag="PC472_002835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728148.1"
                     /translation="MFKKKYIKKPSKISVRNIIAFIITVIGIALGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLVGVISGYLIGKAIYLIASLISYIIYE"
     gene            7948..8178
                     /locus_tag="PC472_002836"
     CDS             7948..8178
                     /locus_tag="PC472_002836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728149.1"
                     /translation="MKEYIIWFKSGNSISGIVDEDVADKLMQDFIEADSDCRNLKGYL
                     DEDGTTIIDLSQIEAISINNCSENNNIGFNPR"
     gene            8219..8782
                     /locus_tag="PC472_002837"
     CDS             8219..8782
                     /locus_tag="PC472_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2728150.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTVTQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            8794..9693
                     /locus_tag="PC472_002838"
     CDS             8794..9693
                     /locus_tag="PC472_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2728151.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWESKTLTNQRKWSTLVHPADIDQTNYVASI
                     GNITQVYNEEQKFPEMDAYCISKIYADWIALGKTAETTALTTANILEVFDKLMEKMTE
                     ARVPAVGRILYVTPGVDTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDSVKIVKVPSNL
                     MKTVYDFTTGWKAGAGAKQIFMSLIHPSAIITPVSYQFSTLDEPRAVTEGKYLYFEES
                     FEDVFILNKKADAIQFVVEGDGA"
     gene            9693..9923
                     /locus_tag="PC472_002839"
                     /pseudo
     CDS             9693..9923
                     /locus_tag="PC472_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9996..10397
                     /locus_tag="PC472_002840"
     CDS             9996..10397
                     /locus_tag="PC472_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728152.1"
                     /translation="MENNLIDDIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESLNFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDTLVNYLLTYGKNKILTFRCLRW"
     gene            10391..10738
                     /locus_tag="PC472_002841"
     CDS             10391..10738
                     /locus_tag="PC472_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728153.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDAITKRTNNKEVIVLENQ
                     PCKLSYKNITSATDGKVAKLEQTIKLFISPNIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELLDDKA"
     gene            10738..11028
                     /locus_tag="PC472_002842"
     CDS             10738..11028
                     /locus_tag="PC472_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2728154.1"
                     /translation="MARGGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            11138..11944
                     /locus_tag="PC472_002843"
     CDS             11138..11944
                     /locus_tag="PC472_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728155.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            12293..12730
                     /locus_tag="PC472_002844"
     CDS             12293..12730
                     /locus_tag="PC472_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728156.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPHSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            12723..12899
                     /locus_tag="PC472_002845"
     CDS             12723..12899
                     /locus_tag="PC472_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728157.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            12900..14210
                     /locus_tag="PC472_002846"
     CDS             12900..14210
                     /locus_tag="PC472_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2728158.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            14231..14707
                     /locus_tag="PC472_002847"
     CDS             14231..14707
                     /locus_tag="PC472_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2728159.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            14760..15017
                     /locus_tag="PC472_002848"
     CDS             14760..15017
                     /locus_tag="PC472_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728160.1"
                     /translation="MIKIKDKYEIKDSISFDYSKKRTIEECVSEIYKKAGKYLIDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            15019..15459
                     /locus_tag="PC472_002849"
     CDS             15019..15459
                     /locus_tag="PC472_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2728161.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFVGEDGKPVEWELKAIDSD
                     RDRQLRKNSAIRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            15782..15940
                     /locus_tag="PC472_002850"
     CDS             15782..15940
                     /locus_tag="PC472_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728162.1"
                     /translation="MDNFLQNVLERLSVSLIVFLVSNLLKKRKKPLKAGTKSGWEFDL
                     KIRFHKFK"
     repeat_region   16718..17605
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16718..16746
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            17757..17945
                     /locus_tag="PC472_002851"
     CDS             17757..17945
                     /locus_tag="PC472_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728163.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            18064..18930
                     /locus_tag="PC472_002852"
     CDS             18064..18930
                     /locus_tag="PC472_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF2728164.1"
                     /translation="MENLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIKLEENEVKGVWETNGSWTGTVNKYTESVIDKVRTWIEENNRPAKIEG
                     EKKNFHVVYKIE"
BASE COUNT         7762 a         1945 c         3556 g         5764 t
ORIGIN      
        1 aataattgct aggttagttt aaagggctaa tctagcaata tgaacagact aggcagggca
       61 tgaggatgct gttagttcaa ttctaactat gttcaaaacc tattaataca ctatatgtag
      121 cagttgaatt aagactaaaa tctcatacaa ttttatctta attcagagtc taaaaaccga
      181 gtggggcttg gtaacctcac tcaccatgca ggtactggtg tttaatctag gttcgattcc
      241 tagaacttgc gacataatat atgtatctcc ctactaaaaa tgctaagttt actccaaact
      301 tagcatttta ctttttaaaa agaaaaaaga aatttttatt gtcataatac tatttgttta
      361 ggtatattat aatgtgcata cttaaaatta aatacttagc aagcatttga attaatatac
      421 ataagatata tgacataatt ttttatagtg taagttattt aaattaatta taaataacag
      481 taattttatt atataaaatg tacatattgt gaataataat aataaaatca tgtacaaaat
      541 gccaactgat aattcctcga aatgtattgc atatttaacg taacgtcagt ataattaaat
      601 tataatagtg aagtggaagg tggtacttat ggctacaaaa agtattttaa aaaatgtaga
      661 tgtaagaaaa aaggcatttg gaagaaatct agtatcagct ctagaaaatg ctaaaaataa
      721 acaagaaaaa gaagttgtat taagtaaaaa atgttcagaa gtaccaaaag ataaaataaa
      781 ggatatattc gggagatttt aatgagtggc tatttaattg taaacttaag taatatgcta
      841 ggagagctgg aggaagaaga agttaaaaaa attctctcca gtttttcttg tccccttaat
      901 aaagatgtag aagaattttt aaaaaacaaa gctattgaat tttctaaaca aggtttggct
      961 agtacacatt tagtgctaac ttcttataaa ggcaagcctg ttatagttgg atatttcact
     1021 ctagctaata agtattttac aataaaaaga aaaacattat caaactcttt agctaggaaa
     1081 atagtgaagt ttggacaata caatgaagaa ctaagaagat atattattgg agcacctttg
     1141 atagggcaaa taggaaagaa ttattcaaat aattataata aattaatcaa gggtgatgaa
     1201 cttctaaaaa ttgcatgtga caagataaaa gcagtacagt tagatatggg tgggaaaata
     1261 gtgtaccttg aatgtgaaga taaacctaaa ttaattgaat tttataagga taatggattt
     1321 gtagactttg gaaaaagaag ccttgataaa gacgaaacag attcgttaga tggggactat
     1381 ttagttcaaa tgttgaaata tctaaaaaaa taaaagtaca taaaatctaa aatgactatc
     1441 ttgatagatg gtcttttttt atacaataaa ttaaaaggag aatgaaatta tggagattaa
     1501 gaaaaacaca caagatgtag tatcagaaag aaaaaattcc ctagattctg aattcaagat
     1561 acctgcaagt ggtgtgtgtt atatggctga gtttataaag gaatctaggg aaatcataaa
     1621 agaattagat aaacattttg aaagttgtct agatgtttta tctaaggcaa gactctaaat
     1681 attttgaata tgctgaatca agcatggttt gccaatctgg gaaatcagta tttttaacta
     1741 taaataaatc aaattcatta tcaggaatag ctaaaaagtc ttcttctgaa ttgactatgt
     1801 aattaccaaa tgcaagtagt tcatcaaaag aatcaaaatt agtgtgttgg ttcataaatt
     1861 tcttgctaag aattattgtg cgaatcttat caaagtcagg ttttaaattt tgttccattc
     1921 tttcaatttt cttttgaaat tgctttaaat ttctaatgtc aatattttta ctcataagaa
     1981 cacctccttt caatagaata ttagcataaa attgtggtga attctgctat tgtcgaacga
     2041 ttgttgaagg atattgtata ataacataga atttactata ctataaggag gtgattatgt
     2101 gggatttgag ataagtggta atttgaattt tgacagtgtt attgatgatt taaaaaaaga
     2161 agttgaaaat aaccctacta tatttacatc acaaaatgtt ggaaataagt tcaaagaaaa
     2221 atgtaaaata tgcgaaaaaa tatctgaatt tgaaatacta gaagatggta aagttaaatg
     2281 tttagaatgt gggactgaat ttgaattgaa tcttaaagta gagtaaacaa aaaaagaatc
     2341 tcaattatga ggttcttttt ttattcccaa aacaaacaaa taaagaggtg gtgatgtgca
     2401 agatgtcaaa gaaaaggtaa aacaagatta cttaaaagga atgaaacaaa aggaaatatc
     2461 atcaaagtat gacattagct taaacacttt aaagtcatgg ataaaaagat acaactgggc
     2521 tagtgaaaaa aagaagggtg cacctataaa taaaagaggt gcaccctttt ctaataaaaa
     2581 ttcagttggt catggtgctc caaaagagaa taagaatgct gaaaagtttg gtttcttttc
     2641 aaaatatcta cccgaagaaa ctagggaatt gatacaagaa atatctataa aagataaatt
     2701 tgatattctt tgggaacaga taacaattca atacgcagca ataataagag cacagaagat
     2761 aatgtatgtt aaaggcaagg aagaaatggt taaagaatta aagaaatatg aaagcacaga
     2821 aaatggtgag aagatagagt atgaatttca atttgcatgg gataggcaag catcttttct
     2881 taatgcacag agtagggcta tgagtgaact tagaagttta attaaacagt atgatgaaat
     2941 gattcataag gattggaatt tggctacaga ggagcagaaa acaagagttg agaagttgaa
     3001 atgtgaagtt gataacctaa gtaaagatga cattggagat gatgagttga aaataagtgt
     3061 agattatggt gatagaaatg atagttagag taaattttaa tccagatttc aaggaagcta
     3121 attttactaa aaaaagatac agagcaatga aaggttcagc agggagtgga aaatctgtta
     3181 atgtagcaca agactatata ctaaagttag gagataagaa gtatcaagga gctaatctat
     3241 tagtagttag aaagtcagaa gctacacata agtattcaac gtatgcagag cttacaggag
     3301 ctataaatcg tatttatggt aaacaagctg ataagtattg gaaaactact ttaaatccct
     3361 tagaaattaa gagtaaagtt actggtaact ctataatttt cagaggagtt aatgatgcaa
     3421 aacaaagaga aaaattaaaa tcaattaact tctcgaaagg aaaattaaca tgggtttggt
     3481 gtgaagaagc tacagaactt atggaaagtg acatagacat actagatgac cgtttaagag
     3541 gtattttaac taatcctaac ctatactatc aaatgacatt tacatttaat ccagtctcag
     3601 ctactcattg gataaaaaga aagtactttg actataaaaa tgatgatata tttactcatc
     3661 atagtactta tctacaaaat agattcatag atgaggctta ctacagaaga atgcaaatga
     3721 gaaaagagca agacccagaa gggtacaaag tctatggtct tggagaatgg ggagaaactg
     3781 gtggagcaat acttaaaaac tatgttatac atgaatttcc tacagaattt gaatactttg
     3841 ataatatgag gttatcacaa gactttggtt ttaatcatgc aaatgcaata cttagaattg
     3901 gctttaagga tggagagtta tatatatgta atgaaatata tgtacatgaa atggatacct
     3961 cagaaataat aacgattgca aatagtaaag gtttagaaaa gagtctattt atgtactgtg
     4021 atagcgctga acccgataga attaagatgt ggaaaagtgc aggatataaa gctaaaggag
     4081 ttaaaaaagg accaggaagt attaaagctc aaatagatta tttaaaacag ttaagaatac
     4141 atgtacatcc tagttgtata aatacaataa aagaaataca acaatggaaa tggaaacaag
     4201 acgaaagaac tggattatat cttgatgaac cagttgaatt tatggatgat gcaatggctg
     4261 ctcttagata ttctatagat aataagctta aaaataatgg aataagcttc ttaaagtaaa
     4321 ggaggtgtta aatatttata taagtgaaac agatttaata aaagttcagt taaaaaaaga
     4381 gagcaccttt aacctagtaa aagtcataga acactacatc ttaaagcata ggccagaaaa
     4441 atataaacaa ggagaagaat actattatgg taatactgat gtaaacaata agagaagata
     4501 ttatctctta gatggagcta aggttgatga ttttactaag gttaataata aagcaattaa
     4561 caactaccat aagcttttag ttgaccaaaa agtgggctat agtgttggaa atccaatcgt
     4621 atttaatgca gatgatgata atctcactaa gcttttaaat gacttactag gagaagagtt
     4681 tgacgataca ataacagaac tatatctcaa tgctagtaat aaaggggttg aatggttaca
     4741 tccatatatt aatagaaaag gtgagtttaa atatgtaata attccagctg aagaagcaat
     4801 tcctatttgg gatagtaaaa gacagaggga attagttgca tttattaggt tttattatat
     4861 tgaagatata gatggaaata aaataaaaag agttgagtac tacacagaaa atgatgtaac
     4921 ttactttgtt gaaagaggta atagttttgt tcaagaattt ttatatgatg aatatggaaa
     4981 aatgactgat atacaagaag gtcattttag aataaataac aaagaacagg gttggggtaa
     5041 agttccattt atacctttta aaaataatga aaagtgtgtc tcagatttaa ctttctataa
     5101 atcattaata gatatatatg acaataatat ttctacacta gcagataact tagatgaaat
     5161 acaagaggtt atttatgtat taaaagaata tccaggaaca agtctacaag agtttataga
     5221 taatataaga tactataaat caattaaagt agatggtgga ggtggagttg ataaactaga
     5281 gataaatata ccagttgaag ctaaaaagga gcttcttgat agattggaaa agaatataat
     5341 tatctttggt caaggagtta atccagaatc tcaaaacaca ggtgacaaat cgggtgtagc
     5401 acttaaattt ttatattcac tacttgactt aaaatgttct aagactgaaa agaagtttaa
     5461 aaaagcaatt agagagcttt tatggtttgt gtgtgagtat ttaaagataa gtggtagtaa
     5521 gagctatgat tataaaacag ttcaaattac ttttaatcac tctatgataa taaatgaagc
     5581 tgaaaagata gatatggcag ctaaatcaac tggaattgta tcagatgaaa ctattgtttc
     5641 taatcatcct tgggttgaag atgttaacga tgaacttgag agacttaaaa aacagaaaga
     5701 agaaagtata aaagaatatg aagatgcttt tccgactaaa aagaaaactg aagaaggtga
     5761 acctttagat gaataatata gattactgga taaagacatt taatcagtta gaagaagatg
     5821 ttcataataa aggagataac tttaacaagg aattagaaaa gcaatatgat atagtacttt
     5881 acaacattct tagagaaata aataattggt atatgaaatt tgctaaggat aataaaataa
     5941 gtatgcaaga agctgaaaag ttattaaatc taagagaatt agctgaactt aaattatcat
     6001 tagaagaata tatcaagtat ggagaagaaa atgtaattag tcaaaagtgg atgaaagaac
     6061 tagaaagtgc agttaaaaga gttcgtataa gcagactaaa agcattagag ttaaaaataa
     6121 ggcatcaagt tgaggtatta tactcaaaag agtatgagga tgtaaatacg cttatagcag
     6181 acacttatca agatagctat tatcatactg catttgaaac tcaaaaaggt attggaattg
     6241 gattaatgat agccatgctt agcatgaata atattaacaa aatagttagt agtccttgga
     6301 ccactgatgg aataacattt ggtaatagaa tttggaataa gcatagacca aggttaatga
     6361 gtgaacttaa taaaggatta aaagaaactt taattaatgg gatatctcct gaaaacttaa
     6421 cgaataaaat taataaaaat tttaatacaa gcaaagaaga agctaagtta ttagtaaaat
     6481 ctgagcttgc cttttttagt tcattaagtc aaagggattg ttttaatagt ctaggtgttg
     6541 agaaatatga aatagtaagt gcattagata gtaaggtttg tgagaaaaga tgtagtcctc
     6601 ttgatggtaa agtaattgaa atgaaatact atgagatagg agttactgct ccaccatttc
     6661 atcctagatg taggtgtgta atagtaccat attttgatgg tgaagaaagt tatagagctg
     6721 caagagaaga agatggtgga acttattatg taccatctag tattaagtat aaagaatggc
     6781 ataagaagta tgttaaaaac acttactaag ttagtaggtg tctttattat gtaaaaaata
     6841 aaatttattg agagggtgat ttgaaatgct taaattatat attttatcaa taattgtatt
     6901 ttgcacaggg ctttatttat tcaacatgaa ggtttatagt aatgaggaat taattgaatt
     6961 acttaaaagt gtgaatatca gaaaaagaaa aaaatataat tttatttttc tagctttatt
     7021 tccactactt aattttattc ttggtgtgat actcatacta tattctttgc tagttagcaa
     7081 tgaagatatt attaaatatt taaggaggaa taaataagat ggctaagttt aaaaagaaag
     7141 cagttgaagt agaagctttt aaactaggtt atgatgtaga accaaaatgg tttattgaga
     7201 atgatagagt ttgtaatttt atgcaagaaa aatgtattaa tggtcatata agttgtgatt
     7261 taaagacact agaaggtact atgagagcta ataaagggga ttacattata caaggtgtaa
     7321 aaggagaaat atatccatgt aaagcagata tatttgaaat gacttatgag aaagttgaat
     7381 atagagaaaa aaataaatta tcaacagaga tgactttaga ttcaacaaac tttcaagaaa
     7441 atattaaaaa agctagaaaa gaattagatt tatttataca aactttagaa aaagcagatg
     7501 ataaaattaa taaactagca gaaaaaatga ataaatgtga ttgtaaagtt gatatagata
     7561 agattgtaaa acagttagtg gaacacctaa gagaaggtat tgaataagtt ttgggggatg
     7621 gaaatatgtt taaaaagaaa tatattaaaa agccaagcaa aataagtgtt agaaatatta
     7681 tagcatttat tattacagtt attggtatag ctttaggtgt ttttataggt atcaacataa
     7741 tcatggctca tgttttagga atagctaaca tggtagataa taatactttt acctgtgtta
     7801 gattagttta tggcttagta ggagttataa gtggatattt aataggaaaa gcaatatacc
     7861 ttatagcttc actaataagc tatattattt atgaataatt tgttcaaaga ttttttatca
     7921 tgtaaaaaca aaaggaggct tattttattg aaagagtata taatttggtt taaaagtggc
     7981 aatagcatat ctggaatagt agatgaagat gttgctgata agttaatgca agattttatt
     8041 gaagctgact cagattgtag gaacttgaaa ggatatttag atgaagatgg aacaacaata
     8101 atagatttat cacaaataga agctatatca ataaataatt gtagcgagaa taataacatt
     8161 ggttttaatc ctagataagg cttttttatt atgtaaaaaa tgaaaggaga catttaaaat
     8221 ggattggtta aaagaattat tggaaggaat aaaaatagag gataacaaga ttgatgtagc
     8281 gtctcttcaa aaatctatag aaaagaaaat aaaagagact acagttactc aagaagatta
     8341 tacaaatctt gaaacacagc ttaatacagc taatgaaact attaaaaagt ttgaaggagg
     8401 tatgacaaaa gaagatgtag agaatctaaa aacaacttat gaaactgata agaaaacttt
     8461 ggaagaaacc tacaaaaaag aaattgaaga aaaggacttt aattactggt taaatgatgc
     8521 ttttaagtct gttaaatgta gggatgaaat agcattaaaa gctcatttag acatggaagc
     8581 actaagaaat agtaaagata gacaaaaagc ttttgaagag caaataaacc ctttgaaaca
     8641 ggataaagat tatttgttta atgcaacact agaaggtgaa gaacctaaaa tagatactat
     8701 aacaccagga caagagccta agataaatga ttttggtttt aattttactg gggtaagacc
     8761 tcatgaaaat aataataaat aggaggaaat aaaatggcag cactaaatta tgcaaaagaa
     8821 tattcaaatg ttttagcaca agcatatcct tatactttaa acttcgggga tttgtatgca
     8881 acaccaaata atggaagata tagatggact ggttctaaaa caatagaaat accaactata
     8941 tctacaactg gaagggtaga ttcaaacaga gatacaatag cagtagctca aagaaactat
     9001 gataatgctt gggaatctaa aacattaact aaccaaagaa aatggtctac attggttcat
     9061 ccagcagata ttgaccaaac taactatgta gcatcaatag gaaatataac tcaagtatac
     9121 aatgaggaac aaaaatttcc tgagatggat gcttactgta tatctaaaat atatgctgat
     9181 tggatagctc taggtaaaac agcagagaca actgctctta caacagcaaa tatcttagag
     9241 gtttttgata agttaatgga aaaaatgaca gaagctagag tacctgctgt tggaagaata
     9301 ttatatgtta caccaggagt tgacacttta atcaagaatg caaaagagat acaaagaact
     9361 gtaaatataa aagatggagg aacttctctt aatcgtcaaa caactgatat tgattcagtt
     9421 aagatagtta aagtgccatc taatcttatg aaaacagtat atgactttac tactggatgg
     9481 aaagcaggtg caggagctaa gcaaatattt atgtctttaa tacatccaag tgcaataatt
     9541 acaccagttt cttatcaatt ttctacatta gatgaaccaa gagcagttac agaaggaaaa
     9601 tatttatact ttgaagaaag ttttgaagat gtatttatat taaataaaaa agctgatgca
     9661 atacaatttg ttgttgaagg agatggagca taatggcaca agtaaggaaa ttaaatagaa
     9721 tattaactat agaagaatgt aaaatagatg atttcttaga aatgggatat gatttaatag
     9781 ataaagctgg aaaagtagag aaatatggta agtctttaaa tgtaaaggat ttggttgctg
     9841 aaaataatat tttaagaaac aaagtagaat ctttagaagt agaaaataag cagcttaaag
     9901 agaaagtaaa aactttagag gaagaagata tccaatctat agaaaaagca gagcctacag
     9961 aaaagaataa gtctaacaaa aagtaggtga aaattatgga aaataatctg attgatgata
    10021 tagaaaaaag acttgaaagt tttggatata tattaaaaga tggggataag tggttaatag
    10081 gttttgtaag agaaaagata gaaaatatta ttaagctgga ttgtaatata aaaactatgc
    10141 caattgaact gaaagaaatt gaagttgata tgatagttgg agagttctta tttactaaaa
    10201 aaaatatggg tcaattagat atagaaagtc ttaattttga agctgtagaa aagtctatat
    10261 cagaaggtga tacaaaggtg gattttgcta taggaagtgg ctctcaaaca ccagaacaac
    10321 gctttgacac tcttgtaaac tatcttttaa cttatggtaa gaataagata ttaactttta
    10381 ggtgtttaag atggtaagta aaactagaaa agcaatagaa atgttatata gagataaatg
    10441 cactatagta gaatatcaac caatcaaaga cgctattaca aaacgaacta acaataaaga
    10501 agtaattgta ttagagaatc aaccatgtaa gctttcatat aaaaatatta catcagctac
    10561 agatggaaaa gtagctaagc tagagcaaac tattaaactc tttatatctc caaatataga
    10621 aattaaagca ggttcaaaac ttattataaa tgataaagag tatgtaagaa gtggagaatc
    10681 agctatatat cctaatcatc aagagataat attagaatta ctggatgata aagcgtaatg
    10741 gctagaggtg gaagtgttga ttttagagag tttaaaagag tttgtaaaaa gatggagaag
    10801 cttacaaaga ttgatttaga taagttttgc aaggatgcag caagagaatt agcagcacga
    10861 ttacttggga aagtaattag aagaacacca gttgatacag gattcttaag acaaggatgg
    10921 aatggagtgg cttacgctag gtcgcttcct gtgtataaac aaggaaataa ttatattata
    10981 gaggttgtta atccgactga atatgcctta acacaatggg gcatataaaa cccagcaaaa
    11041 tcggtaaacg ctaagtgtaa tagtgttaca ttataaagta ggtagaatat ggacatagaa
    11101 taactcttga tttataatgt ataaaagagg tgattttatg gcaaaatttt tagatattac
    11161 aggaaaaaaa tttggaaggc taagagtcat taaattttct aaagagataa aaagtggtaa
    11221 aagaaataga aaatattggt tatgtaagtg tgattgtgga aatttcaaag aaataagaac
    11281 tgactcttta actagtggct tagtacaatc ttgtggatgt ttaaaaaaag aacaagataa
    11341 attaaattta acagacaaat accaatttaa aaagaaatat aaggttcaaa ataaaagact
    11401 ttacagtata tggaagggta taatatctag atgtacagat aaaaataata aaagatacaa
    11461 tagatatggt gaaaggaata taattgtgtg tgatgaatgg ttttgctatg ataattttgc
    11521 aaattgggca ttgagtaatg gatattcaga gaaacttaca attgatagaa taaataatga
    11581 aggaaattat gaatcaagca attgtagatg ggtggatata aagacacaat gtagaaatag
    11641 gtcaacaaat atattagtaa agcatgaaga aaaagaaatt acattgatag agctttctga
    11701 aaaaacaggt atttcatatt cttgcttaag aagtagatat tctaagggat tagtaggaaa
    11761 taagcttata gaaaaagtta agattattga agaaagcaga gctaagttat ctattgagga
    11821 tgtaaaagaa attagaaaaa agtattctga tggatataca ataaaacaac taagtgaaat
    11881 atatcctgta acatattcat ctatatcaaa tatagtccat agaagaacat ggaaaaatat
    11941 ttaattaatt atatatgcca ataccgagga aaacctatag attgcgaata ggctataggt
    12001 ttccgtagag cgtagagagt gaataaatat aatctctcca agagtgctgg gcaactaaat
    12061 aaagtttatt ttgttgatga tgtacgccga acttatagga aactataaga gctagaggat
    12121 aaaaagcctt taggataaca aaatgcatat gttgaatatg gccatagaac taaagatgga
    12181 aaaggttggg ttaaaggaca acatttctta acaatttcag agatggaact acaaagccaa
    12241 gttgataaga ttatagagaa aaaactatta atattgctta aaggagtgtt tgatgcttaa
    12301 taatattata gatggaatat cagtaaagtt agataaatcg tttggagaaa aatatacaat
    12361 ttatagcgaa gatgtggagc aaggtattaa tgaaccttgt ttttttattg ttcctttaaa
    12421 tccaagcaag acaccatatc caagcgggag agaattaaag aaaaattctt ttgatgtaca
    12481 ttatttccct cattcagaag ataagaattt tgaaataaat gagatagctg agatgctact
    12541 ggaggaatta gagtatatag aaattgatgg agatttagtc agaggtacaa atatgaattt
    12601 tgaaattata gacaatgttc ttcacttctt tgttgattat aactacttta ctataaaaag
    12661 taatgacaca gataagatgg atacagtaga gttattcggt ggtttgaaga gaggtgataa
    12721 ttttgagtaa aacattaagc aaagaagata actacaagtt tactaaggag cagatagtta
    12781 actctaagaa gtatgtaaat agaaaagatt tattaaatgc aattttaaaa gaaaatgatt
    12841 tatattcctt ctcagaggta gaggatagaa taaataaatt tatgaaagga gtgagttaga
    12901 tggctttagg tggaggaaca tttgtaacac aaaataaggt cctacctggt gcatatataa
    12961 attttgtaag tgctacaagg gcaaccagtt cattatcgga tagaggtatt gttgcaatac
    13021 ctttagagtt agattggggc atagatgaag acgtatttca agtaaccagt gatgattttg
    13081 agaagtattc agtgaagtat tttggatatg attatactca tgagaagctg aaaggtttga
    13141 gagatttatt caaaaatata aggttgggat atttttataa attaaataaa ggcgttaaag
    13201 ccagttgtac tatagccaca gcaaaatata gtggtatcag aggaaatgac ttaaaagtaa
    13261 cagttacaac aaatatagat gataatgcta agtttgatgt tgtaacactt ttagataata
    13321 agaaggtaga tactcaaata gcaaaggtta ttacagactt acaagacaat gactatatca
    13381 cttggaagaa ggatgcaaca ctagaagcaa gtgcaggact tgtatttact ggtggaacta
    13441 atggcgaagc tgtgacagga gcagagtacc aagctttctt ggataaaata gaaagctata
    13501 gctttaatgc tttaggatgt ttggctacaa caacagaaat taaaagttta tttgtagaat
    13561 ttacaaagag aatgagagat aaggtaggag ctaagtttca aacagtacta tataagaaaa
    13621 gtgatgcaga ttatgaaggt gtagtgtctg tagaaaataa gattaaagat attggattag
    13681 tagaatctag tttaatttat tgggcggctg gagctatagc aggatgcgat ataaataaat
    13741 ctaatactaa taaaaagtat gatggtgagt ttgatgttga tgttaattat acacaaatac
    13801 aacttgaaga agctttaaaa actggtaaat ttatattcca caaggtggga gatgaagttc
    13861 atgtgttaga ggatataaat acttttgtat catttacaga tgataaaaat gacgattttt
    13921 caagtaacca aagtgttaga gtacttgacc aaattgctaa tgatatagcg actttattta
    13981 atacaaagta cttgggtgaa gtaccaaatg ataaatctgg tcgtatctcg ttttggaatg
    14041 atgtagttaa gcatcatgaa caactgcaaa atatgagagc aatagaagat ttcaaagctg
    14101 atgatgtttc tgtagaacct ggaagcgata agaagactgt tgtagtaagt gatgctgtaa
    14161 aagttattag tgctatgagt aagctttata tgactgtttc agttagttaa gagaggagtg
    14221 tgataaatag atgtctaaaa atattactat gaatgttaga gatgcaataa gtggttctaa
    14281 agctgaatgt tttgtaacaa taaaaggtaa aagatataat tttatgcaag ctattaattt
    14341 agaagcaaaa atggaaaaga ataaaagtga gatgccgatt ttaggcagca ttacaaaagg
    14401 aaacaaaagt acaggaagta aattttcagg gagtgcaaca ttttattata atacttctat
    14461 ttttagagag ttgttatatg aatataaaga aacaggcgag gacatttact ttgatatgca
    14521 aattaccaat gaagacccta catcaagtgt cggaagacaa actataatac ttgaagattg
    14581 caacctggat agtggaataa ttgctaagtt tgatgctgat ggcgagtatt tggatgagga
    14641 tgtagatttt acttttgaga attggagaat agttgagaag ttcaatatag caaatggtat
    14701 ggaataagat acacatttat aaattataga tgtgtatttt ttatttacaa aattaggaga
    14761 tgattaaaat taaagataaa tatgagataa aagattcaat ttcttttgat tatagtaaaa
    14821 aaagaactat agaagaatgt gttagtgaaa tatataaaaa agcagggaaa tatcttatag
    14881 atattgctga taaactggca cttgatacaa ttgaaggttc atcattaaaa ccaattacaa
    14941 taaaaatcaa actagatgag gatggcattg caacaataga aaaagaaaca aaatatttaa
    15001 tcatggaggt agaataatat gggagattta aacgcttttt taagtcaaaa tgcaataaaa
    15061 gtagagaata gaaagtatgt ggcaagtgaa aggtttgtag gtgaagatgg aaaaccagta
    15121 gaatgggaac ttaaagcaat agattcagat agagatagac agttaagaaa aaattctgct
    15181 atcagagttc ctgttatagg caaaaaagga aaagcaacag ggcaatacac tagtgaaaca
    15241 gattttaata cttacacttt gaaattatgt gtggaaacta tagtatttcc agatttacat
    15301 gatgcagaac ttcaaaatag ctatggtgta atgggagcag aggaactatt aacaacaatg
    15361 ttaactcctg gcgaatacac agacctttca agtgaagtag gagaggtaaa tggttttgat
    15421 agaacttttg aagataaagt agaagaagca aaaaactaat tgaaggaggc gattatgatg
    15481 ctagtgtagc tcattattgc cttcataaat tcaaatggaa accacatgaa tatacagatt
    15541 taccagacta cgagagggca tttgttgctg cttctataga tattaaagta gaagaagaaa
    15601 taaaagaaga aaaaaagact gctaaagaag ctagaagaag tagaagaaga taaaatatag
    15661 gtaaaatatg taagaattat atgttataat atttttagca agaagatgta atctacaatt
    15721 tatagagtgg agttcataca aaagattatc ctcccaacgt atagaaggga ggtgaatatg
    15781 tatggataat tttttacaaa atgtactaga aagactatct gttagtttaa tagtttttct
    15841 agttagcaat ttacttaaaa aacgtaaaaa accactcaaa gctggcacta agagtggttg
    15901 ggaatttgat ttaaaaatca gattccataa gttcaaataa gtattatgaa cttcactcta
    15961 ctgcaaatag attgtagttc ttcttgtttt tattatacca caaaaaatta taaataaaac
    16021 cgttgtttat ataaaatatt tgaaatgata gcacattccc gttttgggaa catggtcaaa
    16081 actcaaacta tattactggt attatattaa atatttagca tcaaaattaa ataaagcact
    16141 tactttttgg taggtgcttt tttatattca aatctgtgta cttaattgaa atatttggta
    16201 aaatatgtaa gaattatatg atataataat tgtagcaagg aaaataaaat tgaaaagcta
    16261 agggtgattt tctcatataa acgccaaatt ccaatatagg aaggaggtga atttatatgt
    16321 taattaactt tttattgagt atagtagctg gaattgtttc agcttacatt tacgagaaaa
    16381 taaaaaatca cccaaacgcc aataagggtg atttaaagaa ataactttta aattctaatc
    16441 aattagagaa attcaccttt tacaaagtaa attttatctc cttgctatta ttataccaca
    16501 aattggtaca gatattcaaa aataatatat ttatgatata ataaaaatgt agagattttg
    16561 cagtgttcga tttttgtgat aaaatatggc ctaacacttg aaatataatg tattgaggac
    16621 gtatgataag tattatcatt tgcactactc atggttcact gcaaatttga gagaggtgtg
    16681 tatgtgtagg tattggaaat gctaagttta ttttggggtt ttagattaac tatatggaat
    16741 gtaaattaaa cgaaattaaa aatctaattg atagtattac aagagtttta gattaactat
    16801 atggaatgta aatcaagtgg gcgaaagatg attatatacc agaatcactg ttttagatta
    16861 actatatgga atgtaaataa aactgaataa tgcaggtgaa aagttcttaa cagaggtttt
    16921 agattaacta tatggaatgt aaatgggaca gactcaaccc tactatttac aagctttata
    16981 agttttagat taactatatg gaatgtaaat aatatattct attattaaaa gtaattgtat
    17041 cgggtacgtt ttagattaac tatatggaat gtaaatttta tcaaatacac atacatttta
    17101 atattttctt catgttttag attaactata tggaatgtaa attctaaaag gtcacatata
    17161 tttattaaat ctgttgcctt gttttagatt aactatatgg aatgtaaatc agctttacat
    17221 atctatctat gatttttgaa tctccatgtt ttagattaac tatatggaat gtaaattcta
    17281 atttagttgt taaaataaat tccttaccaa ttgttttaga ttaactatat ggaatgtaaa
    17341 ttgagctgtt agataatatc ctagcatttt tatttttcgt tttagattaa ctatatggaa
    17401 tgtaaattat gtacctagaa atttagaaag ttttggagaa atagttttag attaactata
    17461 tggaatgtaa atttcgcaac ttatgatggt gaaatgatta cattaacagg ttttatatta
    17521 actatgtggt atgtaaataa agcaaaagat aggaatgata attttaaaac tccagacttt
    17581 tatattaaca atgtggagaa aaactaaata gaagaaagaa gcacttattt ttggtaggtg
    17641 cttttgtttt gctcaaaatt ggtcggttga gtaaaataat tagaaaaaat tagtaaaaac
    17701 tcttgaaaag tgtcgcgata caatgtataa ttatattatc gcgatacaga aaagaggtga
    17761 aaattattac tgatagcagt agagcagatt acttcaagca gagacgacag aataagaaaa
    17821 cttttagtgt tctactagat agagagaaag tagaaaaaat tgaagaacat ttaaaaaagc
    17881 agaacaagac taaaactatt tggcttgaag aaaagattaa tgaagagtta gaaaaagagg
    17941 aataaaaaat aagagacgtt ctccccgacc aaagattgaa catcccttat tgacgtatat
    18001 tatatacact aactatagta tacgtcattc cttaaaaaaa ttcaattaag gagtgtaata
    18061 gttatggaaa atttaatagt aaaagagttt aatggaagtc aaatttatac ttttatgtgg
    18121 aaagaaaaat cttgttggat agctaatcaa atagttggat tattcgatta tgctgatgta
    18181 tctaaaacaa tacaggattg cataaaagca gaagactttg agattgaaca agagtatgat
    18241 gtattgaaag gaaatgaatt taatgatttt gtaactactt taaatgtagt cgcaaataat
    18301 ataattagta ataaagctag aagtataact attttttatg aagatggttt gtatggattt
    18361 ttacaataca cagataaacc aattggtgta cagtttagaa aatggcttag acgagaagtt
    18421 ttaccaagca taagacaaac tggtgcatac ataactaaca atgctaatcc cgaaaaacta
    18481 agagaaaaag caagtgagat tgagaaatta cagttggctt ataacagtac atctatgcta
    18541 aaagagttat tagatggtgc aggttttgac aataaatcta aattactaac agcaaagaca
    18601 ttatataaaa aggcaggcat tgatttacct atagaaattg aagaagagga atctttcttt
    18661 gacacaaaac aaatagcatc taaactgaaa atatattcta agagtaataa accagcacag
    18721 ttggctgttt gtgagattat taaaaagata aaattagaag aaaatgaagt aaaaggagtt
    18781 tgggaaacaa atggctcttg gactgggact gtaaataagt atacagaaag tgtaatagat
    18841 aaggttagaa cttggataga ggagaataat agaccagcta agattgaagg tgaaaagaag
    18901 aattttcatg tagtgtataa gattgagtaa atttgattgt attaaatagt ttagtttatt
    18961 tttgggggga ttaatacaat gtatgagaat ttacttaagg agtacaattt aaaaactgat
    19021 gaagatg
//
LOCUS       DAMJYC010000077        18984 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJYC010000077 DAMJYC010000000
VERSION     DAMJYC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.078"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..1033)
                     /locus_tag="PC472_002853"
     CDS             complement(<1..1033)
                     /locus_tag="PC472_002853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728165.1"
                     /translation="MYIEELKQNLNNKIGDIINIYLKIYWNRISNRGEKNLYNIYFNR
                     EIKRVLYNKYLENINIDKRDISVVYDCIINDLNSEIDKRIRNSNINELMAILYGIACF
                     QAEFHGRVFITLNNNTSNFSNILHIEKDILRYIERNLKKRFIVDSISATDKSSIQYLI
                     EKCMHFSSDSDNQNNFGVKLFTDVYMMSLIITNLIAEKNMLLSGLCDDISVWINDYMI
                     YNNLDRYYDNEIIYKEYIEDLNFDIRDYDSKIIEKLKNNFFKSYGFRIETIERFLNST
                     NKTIKDTELVHLVEEKTLVKAFCDDSGADIEEIRFLIKYLTITKSIEYDKLKSMENYS
                     NRILEKAFIN"
     gene            complement(1120..2247)
                     /locus_tag="PC472_002854"
     CDS             complement(1120..2247)
                     /locus_tag="PC472_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2728166.1"
                     /translation="MTIPKLEPSDRKREFDKYTSQQINKVVYNYLFEKNMSNRKIEGE
                     ILNLSSKQNKGYQAMSILHHLGINKNHKGVFMGYTIDKAIEVLKEDEIYFKQIINILL
                     ESKNINKYPIIVDSWELLDEKIALKTIDKSTLKHNRTGVPIEIRHFFEINNLGKNEKK
                     NVNFRYDDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRNIFPKYYEKFLEDEIEGKTN
                     LPKIRFVKFDSNNFDIEFIFTSEIEDDVLEEISQDNPLDIDTINIELETEIRKEGKVK
                     YVYGKQYERNPKNRIEAIKYHGTKCVVCGFDFEKTYGNRGKGYIEIHHIRPLSSVGKE
                     SNINPKTDLVPICSNCHRMIHRKKDSVLSIDELKQLIKDKK"
     gene            complement(2493..2768)
                     /locus_tag="PC472_002855"
     CDS             complement(2493..2768)
                     /locus_tag="PC472_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728167.1"
                     /translation="MYGEIIEIYFDYWINEHMYRDILEKDKYYIESWKEIITTNELLE
                     KSLLEEDRRYVLVELNDLFNSLIEFERELSYKFGFKAGVKFIIELLK"
     gene            complement(3016..4342)
                     /locus_tag="PC472_002856"
                     /pseudo
     CDS             complement(3016..4342)
                     /locus_tag="PC472_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(4355..5098)
                     /locus_tag="PC472_002857"
     CDS             complement(4355..5098)
                     /locus_tag="PC472_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728168.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNINNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKASFDWIMSPNNFLK
                     ILEGNYQDKSINVKVGADAYSPDDSQLKW"
     gene            complement(5119..5433)
                     /locus_tag="PC472_002858"
     CDS             complement(5119..5433)
                     /locus_tag="PC472_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728169.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKAKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(5437..5631)
                     /locus_tag="PC472_002859"
     CDS             complement(5437..5631)
                     /locus_tag="PC472_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728170.1"
                     /translation="MKLKLKRIEKGIKQYELAQKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKGE"
     gene            5773..6633
                     /locus_tag="PC472_002860"
     CDS             5773..6633
                     /locus_tag="PC472_002860"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728171.1"
                     /translation="MINNTIGEKIKQYRKSLNMSRDELANKIGCSVHAIAKYEQNQRK
                     PNLDIIKKISTALSIPINRLIIEENDLDISTNNYTNLKEFDSVVKTFDNYSAKLIGNN
                     ISKFRNINNISIKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTNIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIINDFLIDFINKDIDIDNTPVSHYIDKSEFDLVIHIL
                     ENYGYDINTDYKTKFEITDNVDTIKPINLNFSDFKNFSKNLNWVINGLVENFIQENSN
                     GLIENEDEEY"
     gene            6722..7474
                     /locus_tag="PC472_002861"
     CDS             6722..7474
                     /locus_tag="PC472_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2728172.1"
                     /translation="MNKSYIPKYTYKFPTNLPHGDKKYTLTYIRRMISEFVYDMGKLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNSN"
     gene            7464..8094
                     /locus_tag="PC472_002862"
                     /pseudo
     CDS             7464..8094
                     /locus_tag="PC472_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8210..8440)
                     /locus_tag="PC472_002863"
     CDS             complement(8210..8440)
                     /locus_tag="PC472_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728173.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(8446..8925)
                     /locus_tag="PC472_002864"
     CDS             complement(8446..8925)
                     /locus_tag="PC472_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2728174.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(8925..9140)
                     /locus_tag="PC472_002865"
     CDS             complement(8925..9140)
                     /locus_tag="PC472_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2728175.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(9478..10617)
                     /locus_tag="PC472_002866"
     CDS             complement(9478..10617)
                     /locus_tag="PC472_002866"
                     /inference="COORDINATES: protein motif:HMM:NF038233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family RNA-directed DNA polymerase"
                     /protein_id="HDF2728176.1"
                     /translation="MHLKYSNLMTLESLNLPKIYNLESFSTEIGLSTTILYLLSQKQD
                     KFYHQVIIPKTKSGNRTLSIPSISMKIVQKWIKVKILDNIPVSNCAMAFRKGTNYGIK
                     MNAELHKYNTYILKLDLKDFFNNIEREKVFYLFREIGYNNYISNILSNICTFNEVLPQ
                     GGVTSPSISNLICKRLDRRLENLASKRDITYTRYADDLIFSCNDEVLLIKIKNVILDI
                     LKSEGMEINKLKTKFIRPGSRKKITGLHIDKNKVVVPRDTKRRVRAMIHYAITKEDYS
                     KLDEIKGYISFISSIEGNYISKIKSYINKIVNNGQCRLFGPIVESYNKNKIFKNLELV
                     SCIEEMDWVITKGAFYNREKYELKEVIEKSTEYGEDNINGETSPF"
     gene            complement(10804..11685)
                     /locus_tag="PC472_002867"
     CDS             complement(10804..11685)
                     /locus_tag="PC472_002867"
                     /inference="COORDINATES: protein motif:HMM:NF038232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family effector protein"
                     /protein_id="HDF2728177.1"
                     /translation="MKIKNSYLNSVNKIYTDIYKNINKEYIDVFLCGGVSTNKSCIRD
                     MVRIELENNKIRVLYPEDLFMDILSKNKSMDLLSLENFLADNSDVICIIPESPGSLVE
                     LGAFTNNKRTLENLFVVINEKYKTQKSFITTGPIKYIMNEKGSNRITYYNEDLNALIT
                     LLKFKFRKFIRMSKNKTMDVDTIIGQYYFMQLVIYFTKHISNKDLKKLINYIYTENKY
                     EKEKFNMIFSSSLKLLYKNRHIKRDIISGKISLTDKGNIYVEGMIYNLNIKNRGKLYD
                     SIRYGILYSELYGKIPL"
     gene            complement(12030..12379)
                     /gene="ssrA"
                     /locus_tag="PC472_002868"
     tmRNA           complement(12030..12379)
                     /gene="ssrA"
                     /locus_tag="PC472_002868"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12537..12992)
                     /gene="smpB"
                     /locus_tag="PC472_002869"
     CDS             complement(12537..12992)
                     /gene="smpB"
                     /locus_tag="PC472_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2728178.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            13619..14446
                     /locus_tag="PC472_002870"
     CDS             13619..14446
                     /locus_tag="PC472_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2728179.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            15461..15925
                     /locus_tag="PC472_002871"
     CDS             15461..15925
                     /locus_tag="PC472_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728180.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLRLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(16192..16836)
                     /locus_tag="PC472_002872"
     CDS             complement(16192..16836)
                     /locus_tag="PC472_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728181.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(16766..18055)
                     /locus_tag="PC472_002873"
     CDS             complement(16766..18055)
                     /locus_tag="PC472_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728182.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDHILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(18052..18924)
                     /locus_tag="PC472_002874"
     CDS             complement(18052..18924)
                     /locus_tag="PC472_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728183.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         6603 a         2896 c         1959 g         7526 t
ORIGIN      
        1 tattaataaa ggctttttca agtattctat tagaataatt ttccatagat tttaatttat
       61 catattctat agatttagtt atagttagat atttaatcaa aaatcttatt tcttcaatat
      121 cagcacctga atcgtcacaa aaagctttga caagagtttt ttcttctacc aaatgtacta
      181 attctgtatc tttaatagtt ttatttgtag agttcagaaa tcgttctatg gtctcaatcc
      241 taaaaccata tgatttaaaa aaattattct ttaatttctc tataattttg gaatcatagt
      301 ctctaatatc aaaatttaaa tcttcaatat actctttgta tattatttca ttatcataat
      361 atctatctaa attattatat atcatataat catttatcca aacactaata tcatcacaca
      421 atccacttag tagcatattt ttttctgcta ttaaatttgt aattattagg ctcatcatat
      481 aaacatcagt aaataatttt acaccaaaat tattttgatt atcactgtct gatgaaaaat
      541 gcatgcattt ttcaattaaa tattgaattg aacttttatc agttgcactt atagaatcaa
      601 ctataaatct tttttttaaa tttctttcaa tatatcttaa tatatctttt tctatatgta
      661 atatattact aaagttgctt gtattattat tcaatgttat aaatactcta ccatgaaatt
      721 ctgcttgaaa acatgctatt ccatatagaa tggccataag ctcatttata ttactattcc
      781 taattctctt atctatctct gaatttaagt catttattat acaatcatag actacactaa
      841 tatctctttt atctatatta atattttcta aatatttatt atataatacc ctctttatct
      901 ctctattaaa gtatatatta tataaatttt tttctcctct attagatatt ctattccaat
      961 aaatcttcaa atatatgtta ataatatctc ctattttatt atttaaattt tgtttcaact
     1021 cttctatata cattgtagta taaccccact taggctttag atttttttca ttatattaca
     1081 aatttcaatc tattatataa gttcaaaaag tatcaaatat tatttcttat cttttataag
     1141 ttgcttcaac tcatctatac ttaaaacact atcttttttc ctatgaatca ttctatgaca
     1201 atttgaacat attggcacta aatctgtttt aggatttata ttactttctt ttccaacaga
     1261 acttaatggt ctaatatgat gaatttctat gtatccctta cctctgtttc catatgtttt
     1321 ttcgaaatca aacccacata caacacactt tgtaccatga tattttattg cctctattct
     1381 attcttagga ttacgctcat attgttttcc ataaacatat ttaacttttc cttcttttct
     1441 aatttcagtt tctaattcta tattaatagt atcaatgtct aaaggattat cttgacttat
     1501 ttcctctaat acatcatctt ctatttcaga agtaaaaata aattctatat caaaattatt
     1561 actatcaaat tttacaaatc ttattttagg caaatttgtt tttccttcaa tttcatcttc
     1621 taagaatttc tcataatact taggaaatat atttcttaat aaatctctaa acttagattt
     1681 ccaaattatt ctacttcttg gattctctaa tttatccatc tctataactg cactataata
     1741 attatcatca tatctaaaat tcacattttt cttttcattc ttccctaaat tatttatctc
     1801 aaaaaaatgt cttatttcta ttggaacccc tgtcctgttg tgttttaatg tagatttatc
     1861 aattgttttt aaagctattt tctcatctaa taactcccaa gaatcaacta ttataggata
     1921 cttatttata tttttacttt ctaaaagtat attaatgatt tgtttaaaat atatctcatc
     1981 ttctttaagc acttcaattg ctttatcaat tgtatatccc ataaaaaccc ctttatgatt
     2041 tttatttatt cctaaatgat gcaatatact catcgcttga taacctttat tttgttttga
     2101 tgataaattc agtatctctc cctctatctt tctattactc atattttttt caaataaata
     2161 attataaact actttattta tttgctgtga tgtatattta tcaaactctc tttttctatc
     2221 actaggctct aacttaggta ttgtcatatt atcctcctca attgattatt ttagatttta
     2281 ataaaattat tgtatcatat atttattatt aaaatatgtt atgttattta tttaaatata
     2341 aaaataatac aatgtaattg attccacaaa aaattaaaga gggcatcctt aataccctct
     2401 ttataaaata tatttaattc aaggtgttat agtacaattt attcataatc tcaatctaca
     2461 atttaattaa tctactgaaa catacaaaat aattacttca acagctcaat tataaatttt
     2521 actcctgctt taaatccgaa tttataacta agttctcttt caaattctat taatgaatta
     2581 aataaatcat ttagttccac caatacatat cttctatctt cttctaataa actcttctct
     2641 aacaattcat tagttgttat aatttctttc caactttcaa tatagtattt gtctttttct
     2701 aaaatatctc tatacatatg ttcatttatc caataatcaa aatatatttc tataatttca
     2761 ccatacatgt tagtacctcc ttgactttca tctggcactt aatatatact taacttgtgt
     2821 aggttagtgt acattaagta cctaatttat aaaggtagtt gttatattta tatggcaact
     2881 acctttttct aataaatttt tcaatctaaa tactatatat tttaaatctt tattgcatac
     2941 aattaaggat tatatacttt tcttctaaca ctccttaaac aatagtttat atatcactta
     3001 ataaattata ttttttcata ccttcttaca ttccttaaaa taaaacaaac ttcctatata
     3061 ccaataaggt ctaaagcctg attccccatc tctattttta gcaactataa tttcacttaa
     3121 attagctcca aaaccttttt ctctacgtcc taattctttt atatcctcta ttcccattat
     3181 cttacaataa tcaagagcct ctttctctgt aggtctatga atatatataa ctgaattaga
     3241 atccatatat atctgttttg aacttctcat tactctatca ccttttggtc tttcatcctt
     3301 caattcatca ttcaattgag ctaactgtat aattggaata ttaaaatcta aagtaatctt
     3361 ttttatttgc cttgataaat cagcaaattt agcttctcca acttctttac cttcgtaatc
     3421 caaaatctga agataatcaa ttataacaac atctggttta atacttttta tttctctctt
     3481 aattcctgat attgtattga ctgaatcatt aatatagaga ttattttcct gatttaaagt
     3541 acttaaagca tttatgtatt taattatttt cttcttcatt tagatttcta ttcttcattt
     3601 ttacaactga tattccagtt aaagaagcta tgtttctaaa caaaatttgt atatcactca
     3661 tttcctcact tataaaaagt acttttttac cttgaaatac cacatttctc attatctgta
     3721 aggatagtgc tgtttttcct cttccacttt tggccgctat tgtaattaat tcaccactat
     3781 atagacctcc aatagtttca tctaaaaatt ttattcctat attaatacct tcttcaattt
     3841 catcatttat atattcaaag gctctttctg atatagctgt tatactagaa tccttcttaa
     3901 ccaatttctg attgacttca ttaatgtatt tttctaattg atatataagt gtattatcat
     3961 ctatttcccc taatttgaat ttattaaatt tatcaattat atttctattt ttatattttt
     4021 caataactat atcaatataa tgagatacat tacctatagt tggaactatt gttgatagtt
     4081 ttgtcaaata acttatacct ccaacataat ctataagccc ctttttcttt aaatcttctg
     4141 taagagttat tatgtctata ggctcatccc ttttatgtat atctttcact gaattgaata
     4201 tatgtttgtg ttcttgatga taaaacatat cttcacttat taatatttca atctcttcta
     4261 ttttcctcga atctagtaaa atactaccta atatagattg ttctgcttct gtgttcttaa
     4321 ctaaattact catattatcc ataatataaa ctccctacca ctttaattgt gaatcatctg
     4381 gactatatgc atctgcccct actttgacat taattgattt atcttgataa ttgccttcta
     4441 atatcttaag aaaattattt ggactcatta tccaatcaaa acttgccttc caaccttttt
     4501 cattgcttcc atttaaaaag ctagttttac taactatatg aataatttct aatacagtct
     4561 ctttatcata ttcttttatc ctagcattaa ttactttttt tctagcttct gtaagtttct
     4621 ttacttttga tagttctgta caagtaatat tgaatttttc tataatttca ctgtatattt
     4681 tacttttttc attatttata ttcttgttag ttgttagttg cttgttaatt gcatgttgat
     4741 tagtctgttg attttcttca ttagattgat atagctccca gttttcaata gttataagcc
     4801 tatttttgtt tgttgattta tttgttagaa atccatatct ttcaaatttc tttaaagcag
     4861 ttcttacatt ctgaatactt acatcttttg aacacatatt tgctatactt tccaaagatg
     4921 ttattaactg tccagattta acttcatatt ctttacctcc ataaacccat ttattaggct
     4981 tatgattaac tgacagtaat aggtttatga gtatatctct ttgtttggaa ttacaatctt
     5041 tatatagtgg gctttctttt atctttctat gaagctttat ccaacctaga ttactcaaat
     5101 actatcaccc acttaagcct ataattttat gccatttgca ttttcaataa accaatcatc
     5161 tagtttattt tttattatat acactctatt atctatccat atacaaggaa atcctttatt
     5221 tttagaatga cataactgtc taaattttga ttcccctatg ggataaattt tagaagcttc
     5281 tttacaagtg ataagttgag ctttatctct tctatatgtc tgtatctcct tgtacccttt
     5341 tgccttttct tgtgcttctt gttcttgttt gacaagtata ttcaatgtag cttgtagtgt
     5401 tttaagaact tctagattat ttgtatctgc catagatcat tcccccttat caaaaaataa
     5461 ttcttctaca cttgtatcta gttcttgagc caattttaac atcaacatat tacttggatt
     5521 tttggatttg cctctttcta aagaagataa ataatatctt gaaatgccaa ctttctgagc
     5581 taattcatat tgctttatac ctttttcaat tctttttaat ttaagcttca ttgatttcct
     5641 ccttcctcac atacccatat tgtgcctttt tatctttcta caaaacagaa taacacccat
     5701 ttcgtgcttt atcaatatat ttaagtgtat taatacaccc atttcgtgct ataatgtatt
     5761 tgaggtgaaa ttatgataaa taatacaatt ggagaaaaga ttaaacaata tagaaaatca
     5821 ttaaatatgt caagagatga actagctaac aaaattggtt gctcagttca tgctatagct
     5881 aaatatgaac aaaaccaaag aaaacctaat ttagacatta taaaaaaaat atctactgct
     5941 ttatcaattc ccataaatag acttattatt gaagaaaatg atttagatat ttcaacaaat
     6001 aattatacta atttaaaaga atttgattca gttgtaaaaa catttgataa ttactcagca
     6061 aaactcatcg gaaataatat aagcaaattt agaaacataa ataatatctc tattaaagag
     6121 ctctcagaaa aaataaattg ctctgagaat gaaattataa attatgaaaa tggaacaaaa
     6181 atgcctagaa ttgaaatatt aaaaaaaata gcacttgcta cttataccaa tattgaagat
     6241 ttatttggat tcccattaga agagataaaa atagataacc aaacaatcct ttatagaaac
     6301 cctcagactg gatatataat aaatgacttt cttattgatt ttataaataa agatattgac
     6361 atagataata ctccagtttc tcactatata gacaagtctg agtttgattt agtgattcat
     6421 atattagaaa attatggtta tgatataaat acagactata aaacaaagtt tgaaattaca
     6481 gataatgtag atacaataaa accaattaac ctaaacttct ctgatttcaa aaacttctct
     6541 aaaaatctaa attgggtgat aaatggtctt gttgaaaatt ttatacaaga aaattctaat
     6601 ggtcttattg aaaatgaaga tgaggaatat tagtactatg cattttcaca atataaattt
     6661 aattgttaat tttgatatag tcataattag ataagaatta aataaggaag gagctacaat
     6721 catgaataaa tcatatatac caaaatacac atataaattc cctactaatc tacctcatgg
     6781 agataaaaaa tatactttaa catacattag aagaatgata agtgaatttg tatatgatat
     6841 gggtaaacta gaaaataacc cctttacatt cccagaagta caaacattac ttgatggtat
     6901 aacagtaggt ggacataaat taagtgatca aaatcaaata ctaaatataa aatcatcatg
     6961 ggattatgta ataaaattaa gtaacaaaga aaacttatct ttaaataaag atacaatttg
     7021 ctctatacat aaaatagttg caaaagatga agctctaatc gttggagatt tcagaaatgg
     7081 taatataggt atagctggta ctactcaata tgaatgcata gaagccactc tattaaatga
     7141 tttatttgta aatgacataa atatcataaa taaaataact aacccattag aaaaggctat
     7201 aataataaat ttatggttgt cctattgcca attcttctat gatggcaaca aaagaactgc
     7261 tagacttacc tcaaacctaa tacttataag taatgatata ggagttctat caataccagc
     7321 taaacataaa caagaataca acacattaat gctaaacttc tatgaaacac tagaagctga
     7381 tgaagttata aaattcttat tagaaaaatg tattacattt tttgatggat ataactataa
     7441 atcatataag gagctgttta aaaatagcaa ctaaaagagc taatggagaa tgctctatcg
     7501 gaaaatataa agatggttgg cgttcccgta taatgattgg ctataatgaa gatggtaaac
     7561 caataagaaa agaattttat ggtaagactc aaaaggaagt taaagataaa cttgatgctt
     7621 ataaaaaaca atattattta agctctgata tttcagatga taaaataact ttagagcatt
     7681 ggttttacaa ttggttattt gaatatagaa taaaagattt aaaacctaag tcatttgaaa
     7741 gatatgaaag catctatcat aattatatta aagatactga tttaggtaat gttaaattaa
     7801 aggacttaag agtatctcac atacaaaaat attacaatag attgcttgat tccaataaaa
     7861 gtatacctac tataaggcaa ataaacacta aactgaaaac ctgcttatca gaagctgaaa
     7921 aacaaggtct tatacaacgt aattattgta ctatggtaaa tataccagtt gagaaaactg
     7981 tacaagtttt aatgggtcat tatgatatat caataacttt aaatatatac tcaagttatg
     8041 gaagacaaga aagttgaagc tgtagaaaag ttggataaaa tattttcatt gtaaaaatca
     8101 aatacatata aatattgtaa tttcatatag ttaattacaa aatcaaaata aggatacaaa
     8161 ttaattttat attatcttgt atccttattt cgtatctagt tttaattatt tatcttggat
     8221 agcctttatg atacattaat ccattttcta acattaattc ttgagccaag tatatatgac
     8281 ccttacagtg catatctgcc acaccttcac ttataagttt atatgtttca gaagtatata
     8341 aataatccat actttcttcc aagctcttac ctgtttgctt agaaaattct aatatgattt
     8401 ttttatatag tatttgtaat aatgttttat ttgcttccat aatcactata cctcctcttc
     8461 atacctaaac tttaaatatt tatctattac attttgattg gttatacata tttgttcatt
     8521 tggtttatag tattttaatc tttttaaagc ttcatctttt tcaataagat tatcctgata
     8581 cagttctatt gtattgtaaa tcttatcatc agcaatacca cctattataa tgtcatattt
     8641 catatatata tcactcatat ttctacattc tattataaaa tcaagccatt ctaaattata
     8701 actattaaag atttttacct tgcagttttc ttttaattta tcaatatcta aagaataact
     8761 atttaaatat gcttttttac ctcttttttt aaaacgctct gtccagttaa ttgcttgtgt
     8821 ctttatactg gttacataga atccttttcc aaaatctaaa gaatctcttg aaaaggtaat
     8881 actaggagtc tttaccacac aatatgaacc atgatataca atcattatat cacacccttt
     8941 tctttaagta cttgcattaa atcttccact atgtaacctt tactttgaga atgtagtgct
     9001 tcatagcaag gtattatata gttatctaaa atatcagtat catgtttaag aagtttataa
     9061 actccttctg aatctatatt caatttttct gctaaagctt caatgcaaaa aatagaaaat
     9121 tctagttttc tttcatccat tacaaacctc aactcctatc aagcttatat aatatatatt
     9181 tttactaatt aacaacttaa actgtcttac atttataaaa atattatatc ataaagtaga
     9241 tattaaaatt ctgcattagt taatctctaa taaacaaata aagacttttt atacaataaa
     9301 atccctccca atgttctttc aaaaaagctc tttttttgta atcgaaatac aatttaatgg
     9361 atttcatcac ataaataaaa aatactagcc aataaagact agcattttca attattttat
     9421 cattcatttt tcaaaataga taaatactat ttacaaaact cccacaatca cttttattta
     9481 aaatggactt gtttcaccat ttatattgtc ttcaccatat tcagttgatt tttctataac
     9541 ttctttcaat tcgtattttt ctctattata aaaagcaccc tttgttatta cccaatccat
     9601 ttcttcaata caacttacta attctaaatt tttgaaaatt ttatttttat tatatgattc
     9661 aacaatagga ccaaataacc tacattgtcc attattaaca attttattta tgtaagattt
     9721 tatctttgat atataatttc cctctataga tgaaataaaa gatatatacc cttttatttc
     9781 atctaacttt gaataatcct ctttagttat tgcataatgt atcattgctc tgactcttcg
     9841 cttagtatct cttggcacta cgaccttatt cttatctata tgtaagcctg taattttttt
     9901 tcttgaacct ggtctaataa actttgtctt taatttattt atttccatac cttcagattt
     9961 caatatatca agtattacat ttttaatttt aattaataaa acttcatcat tacatgaaaa
    10021 tattaaatca tcagcatatc ttgtataagt aatatctctt ttgcttgcta aattttctaa
    10081 ccttctatct aatctcttac aaattaagtt agatatagaa ggtgatgtta ctccaccctg
    10141 aggcaatact tcattaaaag tgcatatatt acttaatata tttgagatgt aattattata
    10201 tccaatctct ctaaacaaat aaaatacctt ttctctctct atattgttaa aaaaatcttt
    10261 caaatctaat ttaagtatat atgtattata cttatgcaat tctgcattca ttttaatacc
    10321 ataattagtt ccttttctaa aagccatagc acaatttgaa actggtatat tatctaaaat
    10381 ttttactttt atccattttt gaactatttt cattgatata cttggtatac tcaatgttcg
    10441 attgcctgat ttagtttttg gtattattac ttgatgataa aatttatctt gtttttgaga
    10501 taataaataa agaatggtag tactaagccc aatctcagta ctaaatgatt ctagattata
    10561 tattttaggc aaatttaatg attctaatgt cattaaatta ctatatttta aatgcataat
    10621 gacaagtacc tccttaccat aaatcttttg agttaattgt ttcaattttt catacttaat
    10681 gttgaataaa ttgaaacaat tacgaaatat aaatacactt aaattctaac agagacgaat
    10741 catctctaac tataataagt accatataac atgcgcatct atagcaactt ataacagtgt
    10801 tctctaaaga ggtatcttgc catataattc tgaatatagt ataccatacc ttatagaatc
    10861 atacaacttt cctctatttt ttatatttaa attgtatatc attccctcaa catatatatt
    10921 tcctttatca gttaaagata ttttaccact tataatatct ctcttgatat gtctattttt
    10981 atataataat tttaaagaag aactaaatat catattaaat ttttccttct catatttatt
    11041 ttcagtatat atatagttaa ttaatttttt caagtcttta ttagatatat gctttgtaaa
    11101 atatatcact aattgcataa aataatattg tcctattata gtatccacat ccatagtttt
    11161 attttttgac attcttataa actttctaaa tttaaatttt aaaagtgtta tcagtgcatt
    11221 taaatcttca ttataatatg taattctatt acttcctttt tcgttcataa tatactttat
    11281 tggtccagtc gttataaaac ttttttgtgt tttatatttt tcattaatta ctacaaataa
    11341 attctccaat gttcttttat tattagtgaa tgctcctagt tctactaatg atcctgggct
    11401 ttcaggaatt atacaaatta catcactatt atcagctaag aagttctcta aactaagtaa
    11461 gtccatagac ttatttttac taagtatatc cataaataaa tcttcaggat ataaaactcg
    11521 tattttatta ttttcaagtt ctatcctaac catatctctt atacaacttt tattagtact
    11581 tactccccca cataagaata catctatata ttctttatta atatttttat aaatatctgt
    11641 atatatttta ttaactgaat tcaagtaact attttttatt ttcacttaat cctccatatt
    11701 atttagttta ttcttattct actaattata ctatttttaa acaaatatat tttagatacg
    11761 tataggtagc aataatctat atactattaa ttatatcaat tatttagaag atttaaaatc
    11821 aatgtcttat cttaattcta ttatttatat gtataatact atttttagta tatttataat
    11881 ttaatgtata attatacact taaatgcacc ccaatatacc cccaaaaaaa cctctttttc
    11941 tgtggttggg gtacggttgg tggattttac tccaaaataa aaaatactag tcaataaaga
    12001 ctagtatttc atcattcttt tttcaaaatt ggtggagacg aggggagtcg aacccctgtc
    12061 cgcaaatctt ttccttgctg catctacatg cttagccaac ttcactaata tctcacctca
    12121 catagcgcca agtggcaggc tctatgctta gccagtccca attaatctac ttcaagtcga
    12181 gacaccccta aagtagttcc ctgcataatg acgtccagtc ttagtcggca ggaggactaa
    12241 gagggacgag cagcaactag gctgctatcg cgaaattatc gtctgcgttt attttaaagt
    12301 tccccagttt ttacgtgacc cagagtaaca cgacatgcag cctcaagttc caaacccacg
    12361 tcgaaacctt tacgccccct tgagtagttt atagtccagt agacttttcg caaactttat
    12421 caccattttt attaaatttt taatgtatat ataatataat acttttaaga ataaaaatca
    12481 atgttatgtt ctagataata aagaatttta ttctaaaaaa tacagtttta tctagtttaa
    12541 tacttacctg tcatttctct ttctatacgt ctttgtgcat ctttcttagc catatcttga
    12601 cgtttatcat atagtttttt accttttgct aaggcaatct ctactttaac tttaccgttt
    12661 tttagataca aactaagtgg tataagtgaa taccccttta tagtagttgc tccaataagt
    12721 tttcttattt catgcttatg aagtaataat tttctaactc ttaaagaatc aacattgaat
    12781 atatttcctt gctcatatgg acttatatga acttgtttta aaaatacttc gctattatct
    12841 acagatgcaa atccatcact taggttaact ttgccttgtc ttattgattt tacttctgtt
    12901 ccttttaatt caattccaca ttcataagtt tcttctataa aatattcatg tcttgctctt
    12961 ttgtttgttg ctaatatttt tttaccagcc atataaatct caccttcttt gctagaaatt
    13021 tgttccataa atgttagaaa tttactaaat atttaatgtt gccaaattat ttgattaact
    13081 tgtatgataa catatttagg cattttagtc aatatttcac tgatatatta agttagttag
    13141 gctatatata attaagtaat ataaatgtat gaaaatagct aaaaaactac ttcgaatata
    13201 cttaatacta aactaactct aaccagtaat actaatttat aaatttcaaa acaaaaatat
    13261 tacatattat aaatcttcta tcttctatct tctatcttct atcttctatc ttctatcttc
    13321 tatcttctat cttctatctt ctatcttcta tcttctatct tctatcttct atcttctaaa
    13381 taattttaac atgcccataa aatataatac ccacaaatga taaataatac tcaaaatgtc
    13441 tattgaaata actatacatc cttaaataac ttaacttaaa tctaaacatt tcaaaaactt
    13501 cttaaaaata tgtaaaaatt tgatgataaa gtagcgattt tttcaaaaaa aatgtaatat
    13561 agagttatat cttataatat tttagagata aattttatca tagtggggag aaatttgtat
    13621 ggatttaagc aaaaaagtaa aaattttaga aaatgcatca gatggaaaaa gcgaatttga
    13681 gatattagaa tttgaaaaac ttgagggagc ttctagccca ttaatggcaa tgggattata
    13741 ttttgctaga gaagctggtt tgaaagctag acaaattaag ataaaattaa ataattcatc
    13801 aataaaaaca gaagctggtg cattatacta ttataaagga aatatatctt ctgaaactaa
    13861 gattggtggt gttggaggtc tttttaaaaa aggaatatca ggagcactta ctaatgaatc
    13921 aatgatgaaa cctgtataca aaggtactgg agaagtttat ttggaacctt cttttagaca
    13981 ttatttattt atgagtcttg ataatgattc tataattgtt gataaaggat tattctattg
    14041 ttgctctgat acaatagaag taaaagcatt tacacaaaag aacttctcat ctgctttact
    14101 tggtggtgaa ggcttcttcc aaatagaatt aagtggtact ggtgtaatcg ttctggaatg
    14161 tgttgtacct caaagtgaaa ttgtagagta tgaattgaaa aatggtgaag aattaaaggt
    14221 agatggtaac tttgcaatag caagaacatc tggtgttaat ttttctgtta caaaatcaga
    14281 taaatcttta attggttctg ctttaaatgg tgaaggtttc ttaaatacat ttactggtag
    14341 tggtacagtt tggctagctc caactcaagt aatgtatgaa aaactaagtt ttggagcaat
    14401 gcctactaat gataatatga ataataaagt ttctaagcaa aaatagttgt tttataaata
    14461 tttattaaat cattattgat tttataaagt cactataaat tttattagat tattattata
    14521 aaatcagagt gtggaaatgt ttatgtgtat tctttaaata aaaatttcca cattctgtaa
    14581 ggttattata cttgatatat aaaattcaat ttaggaacat agtctaacat ttataaacat
    14641 attaaggtaa tgatttctct tagtaattat tctcatatct tattctttga ttatatttat
    14701 cttattccta gcccttatgt catattatat ttcttatctt attatttata attagcatta
    14761 acttatctaa aatcttgtat ttttttaata gtaaagttta catctatatt aaaaagttta
    14821 catctatatt aatttcataa aacaatcaag gtagtcaaga gattcaactt tatttgattt
    14881 taaaaattta agtgaatttg ttcttataca catcttttta aaattatatc tgtagaaaat
    14941 atcatttaga gctataatta aattaactta ttaaatttta gaaaatttat taaaaaatat
    15001 aagttaaatt aatattttta aagattcttt tttataacta ctagaatagt aatttagtag
    15061 ttattgtatc tctaaagttt aagcatggat agatatacat ttgataattt gcctataatt
    15121 tacctactac ttggagtaaa tacaatatat taataataag ttcctaaagc caataaatta
    15181 actaatacac ataaagatat tgatgttgat tatacaacta taagaaacgg atttaacaga
    15241 atttaatcaa gctaagaaat tatatatatc gtgatgtttt tgaattagaa ggtaattcaa
    15301 aattagttta tagtcttctg tgaatccaat aaaaagtaca gggtacaaga attgttagta
    15361 aaatttttaa agattttatg gataaatata agtagtttta cttattgtct aaaactttaa
    15421 aataccatta attaccttaa atattttaat aggaggacat atgaatttaa ataaagttaa
    15481 atttactgaa gaacatgcta ttgaagttac taattggaaa tatgaaggta aatattcaat
    15541 atacaatcta cctccatggg aggaaattaa aaagaagaat ttttcactag caaaagaaga
    15601 taaaagaaaa aattttataa gttttataaa tgacaataaa gaattgatag gttttataaa
    15661 cttattagat gaaggaagct ctgtattctt tggtattggt atgaaaccga attattgtgg
    15721 aatgggaatt ggtaaagaaa taattagcct aagattacaa gaatgtagaa ataaatatag
    15781 tactaaacca attgtattaa atgtaagaac ctggaacatg agagctgtaa aatgttatga
    15841 gtctcaagga tttaaaatag ttgagactaa ggtacaaaaa acgcatttag gagatggaga
    15901 attttttgtt atgaggtatc aatagtttat aacaatagtc ttattgttaa tacaacaatt
    15961 aaaataaaaa aatacatatg tgtaggtcat agcaataagt gtctttgaaa tataaatttg
    16021 agagcttttg ataaaatttg gtatacatat atattttata ctacggatat ataaatctat
    16081 ctacatacat aaagagattg taagtaggtt tgtgtatata aaaatagatt tatatttcat
    16141 aaatgtacct tttatttgta ttaactttat tttatctaag ctctcatttt attatctaat
    16201 cttccttttt actggtttta ttaagagaga tgcaattatc aacacaacac ttccaagtat
    16261 cagccaatat ttctcatcaa aactaccttg actcatagac attataaaaa attttccaac
    16321 atacttgcct ttagtcatca tatttaaaat taaatatcct atagatatca tataacctat
    16381 gattgtatta ttaaataaat ttgagaaaaa cattcctaaa cttcctataa acatagctga
    16441 tgcgaaactt ccaataatat actcaactac tggaaaatta catccaagat atattaaaac
    16501 atttacaaaa cctatgatta ataccactat acctataatt gacatcagta tcctaattaa
    16561 ttgtacaata acttgagata atgcttttga agatactatc tcatcaattt cttttgattg
    16621 ctctagcgaa aatacaggta ccaacattac tatccctatt attgcaacat aattttctaa
    16681 acatatagct gttgaaattc tgtctaaatt agctactcct tgtattagag gaatcataca
    16741 tagcaatata attgaacata ttaaattagt taaagtgctt aatttgaagt tttcttttat
    16801 tagaactaaa cagttttgaa aactttgccc aaacatcaag attccccctt cttttttgct
    16861 catataagaa tatagctaac aaaacaagca ccaatgcttc caagcttatc acaattctat
    16921 taatcataat ttgattgaag ttttgtataa taatatctcc atttaataca ctattatgtc
    16981 ttatcattag tcccattgtt gaataatcac ctttgagtcc tgtagaattt atactgtaaa
    17041 gccaccaaat taattgcaca ataattgcta ctggtgtatc tgttaatata gtaatactca
    17101 ttcctacagc tgtaactatc ataagagttg gtagtatcca ccataatgta tattttataa
    17161 atgctaagtt atcaatagat atacctttat ctttagcaaa gaaaatcaaa ggaagtaaat
    17221 ctttcatacc taaaattaat acaggcaata tcatcataaa gacaagtgca aaatatcttg
    17281 ataaaactat tttgcatgaa gatacttgtt ttgaataaat aatctctctc attcttgaac
    17341 gcttatcttt aagtgacata aaaactgcca caaatactgg aaatatactt aaaactatac
    17401 ctatataatc gcaaaaaatt cttgcatacc cattactaac tttatctttt tgtattgtat
    17461 agttgtaatt cttcatagct tcatcatagg taactggaac tactccaaat ctttgtaact
    17521 tgtcaaaagc atatgcagac cctcctccta aaatgtggtc tactctttgc attatttctt
    17581 caaatctttc aagagatact ttagaataat ctatttttat actttcatca gttttccaaa
    17641 gtgaatttat ttcgctaata cttttaccag ttatttcttc tatatatttt tctatggtct
    17701 tcgttttatt ttggtctaat tttatatact taactacccc tgttggatac gttgtatatg
    17761 tgtttttttg atattctcta attaggttta tagttgcatt tggaatgacc ttttctgttt
    17821 tcatagattc tttttgacca tatggatatt gattgtgtac agaataatat tcctcaatat
    17881 tctctacttt ttgtggagca actaatggat ttccatcatt aggaatattt ttacttacat
    17941 catttgaaac tgaacttcca agttgagaaa tatagaaaaa aactaaaact acaataaaaa
    18001 tataatatac catactcttt aatatatttt tacattcttt tataaataga ttcatatatt
    18061 atctcctccc attataagca tatagccatc ttcaatagtt ggattacaat ttactgctgt
    18121 ctcaaaaggt ttatcatctg atataatcct aagtatagcc tttccaccat gagtcaacat
    18181 tcctataact ataaatctac tttttatatt ttcaatgtct tttttatcta cttcaatact
    18241 atatactttc ccttcagcta gttttgctaa tgcttctgtt gagccattgt atatgacttt
    18301 tccactattc aatatagcta cgttttcaca tgtagcttct atatctccaa caatatgtgt
    18361 agataaaata acgattctat catttgcaat ttcgcatagt aaatttctaa atcttatacg
    18421 ttcttctggg tcaagacctg ctgtcggctc atctactatt aaaactctgg gattatgaat
    18481 taatgcttgt gctatcccaa gccttctatt catacctcca gataaagatt ttactttcac
    18541 attttgataa ttagtaaggt ttactttgct taataacttt gctataattt ttcttctttt
    18601 tcttagtggt atatccgata aaactgctaa ataatccatt gctttatata ctgtcatgtt
    18661 aggatacatt gaaaaatctt gtggcaaata accaaccata tttctaattt ccttagtatt
    18721 ctcaatagga actccacaca tatgtattga accttctgtt tttgataata aagtggctaa
    18781 aattctcata aatgtggttt ttcctgctcc atttcttcct aaaagaccaa acataccact
    18841 gtcgatttca agatttatat tatcaagagc ttgttttttc ccaaatcttt tactaagatt
    18901 ggatactgct attttcattt ccatatataa atacaccccc agaaataatt ttatagaatt
    18961 ttatttttat aaatttttac tgta
//
LOCUS       DAMJYC010000078        18955 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJYC010000078 DAMJYC010000000
VERSION     DAMJYC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.079"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            297..641
                     /locus_tag="PC472_002875"
     CDS             297..641
                     /locus_tag="PC472_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2728184.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            672..2273
                     /locus_tag="PC472_002876"
     CDS             672..2273
                     /locus_tag="PC472_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2728185.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2292..3392
                     /gene="dprA"
                     /locus_tag="PC472_002877"
     CDS             2292..3392
                     /gene="dprA"
                     /locus_tag="PC472_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2728186.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3425..5512
                     /gene="topA"
                     /locus_tag="PC472_002878"
     CDS             3425..5512
                     /gene="topA"
                     /locus_tag="PC472_002878"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2728187.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5677..6462
                     /gene="codY"
                     /locus_tag="PC472_002879"
     CDS             5677..6462
                     /gene="codY"
                     /locus_tag="PC472_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2728188.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6569..7834
                     /locus_tag="PC472_002880"
     CDS             6569..7834
                     /locus_tag="PC472_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2728189.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7865..8635
                     /locus_tag="PC472_002881"
     CDS             7865..8635
                     /locus_tag="PC472_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728190.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8744..9190
                     /locus_tag="PC472_002882"
     CDS             8744..9190
                     /locus_tag="PC472_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2728191.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9208..10401
                     /gene="nifS"
                     /locus_tag="PC472_002883"
     CDS             9208..10401
                     /gene="nifS"
                     /locus_tag="PC472_002883"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2728192.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGIAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10455..10895
                     /gene="nifU"
                     /locus_tag="PC472_002884"
     CDS             10455..10895
                     /gene="nifU"
                     /locus_tag="PC472_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2728193.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11184..12263
                     /gene="mnmA"
                     /locus_tag="PC472_002885"
     CDS             11184..12263
                     /gene="mnmA"
                     /locus_tag="PC472_002885"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2728194.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12406..12644
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12712..15351
                     /gene="alaS"
                     /locus_tag="PC472_002886"
     CDS             12712..15351
                     /gene="alaS"
                     /locus_tag="PC472_002886"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2728195.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15376..15633
                     /locus_tag="PC472_002887"
     CDS             15376..15633
                     /locus_tag="PC472_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2728196.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15643..17814
                     /locus_tag="PC472_002888"
     CDS             15643..17814
                     /locus_tag="PC472_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728197.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18020..18445
                     /gene="ruvX"
                     /locus_tag="PC472_002889"
     CDS             18020..18445
                     /gene="ruvX"
                     /locus_tag="PC472_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2728198.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18523..18810
                     /locus_tag="PC472_002890"
     CDS             18523..18810
                     /locus_tag="PC472_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2728199.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
BASE COUNT         7783 a         1916 c         3578 g         5678 t
ORIGIN      
        1 attatcatag ttatgaccgg atacaatgta ttttcaacat ttttcaaata gcttacaact
       61 aatataaata aagataaata tcgtataaaa gcaactaatt tttctctgtt cattaaaggc
      121 actcctccaa aaaaataaac ttttgctatg aaaactagaa taatatcata aaagaataaa
      181 gtcaatagaa gtatttatta atattgatgg aaataaaatc aatccaaaaa cttgaatagt
      241 aaaattgtat actgacaata atttaaaagt gaaaatttaa tgtattggag tgaataatga
      301 ataataaaga aaaaggtgat tttggagaaa aagtagcagt taattatttg ctatcaaaag
      361 gagcaaagat actagaaaaa aattacagat taaagatagg ggaaatagat ataatagcaa
      421 aaatggaaga tgaaattata tttgtggagg tgaaatcaag gtctaacata aagtttggct
      481 acccttgtga atctgtaagc ttcaaaaaaa gaaaaaagat aattggagtt gcaagttatt
      541 atattataaa aaataactta aataacactc ccataagatt tgatgttata gaggtatatt
      601 tattagaaaa aagaataaat cacataatga atgcttttta aaaaagataa tgtttttgga
      661 aaggattaaa tatgttaagt attattaatt caagcaactt agtaggaatc gacagctttt
      721 tagttaaggt tgaagtagat gtaagcaatg gaatacctag ttttaatatt gttggtcttc
      781 caggcaaaga aattaaagaa gcaagagaaa gagtaaagtc agcaattctt aatagtggat
      841 ataaatttcc aagcacgaga atagttgtaa atttatcacc agcagatata aaaaaagaag
      901 gagctttttt agatttatct ataagtatag gccttttaag agagttaata aaaaaagatg
      961 aaaattacat aagagaaagt atgtttatag gagaattatc attagatggg aaaataagaa
     1021 aagtaagagg tatattgccc ataattatgg gtgcaaaaac tcagaacata aaacgtattt
     1081 ttataccaat tgagaacata aaagaaagtc tatttgtaga tgaaattgat ataattccta
     1141 ttaaaagttt aaaagaatgt gtcgattttt taaacgaaga gataaaagtc gataaagtaa
     1201 ggataatgtc ttttttggat gataagtcaa gaaaagagaa cgaagaatta gaaaaagata
     1261 atagttatat agattgcaaa tatactaaga ttaataatga agaaagtaaa tatgatgaag
     1321 actttaaaga tgtaaaagga aattattttg taaagaggag tgcagaaatt gctgctgctg
     1381 gaaatcataa tatgtttatg attggacctc caggctcagg aaaaactatg attgcaaaaa
     1441 gagtaaggac aattttacca gatataagtg tagaagaaat gatagaagta agtaaggtct
     1501 atagtatatt gggtatgata aatgaaacta aaggaataat tgataaaaga ccttttagag
     1561 cacctcatca tacaactaca aagcaatctt taattggagg aggaatggat gcaagacctg
     1621 gagaaattgc tttagcacat agaggtatct tatttttaga tgagatagcc gaatttgata
     1681 gaaaaatact tgagacttta agacaaccta tagaagatgg atatgtaaat atatccagag
     1741 taaagtacag tgcaaaatat ccttgtagag tactgttagt tgcagctatg aatccatgtc
     1801 catgtggtta ttatatgtca gagacagagt gtagatgtag aagtaacgaa attgatagat
     1861 acataaataa aatatcaggt ccattgttag ataggtttga tatttttgta gaagttaatt
     1921 ctataaaata ttcagatttt aattctttaa aacaagaaga aagctctcaa aaaataaaga
     1981 gaagagtaga aaatgctaga aaaatacaga taaatagatt taagaaagat aatataaaaa
     2041 ataatagtga aattaaagct tacaatttat ttaaatattg taaactggaa aaagaagctt
     2101 caaaaacggc tgagatgata tttagtaaat acaacctaag cagtagaagt tatacaaaac
     2161 ttttaaaaat ggcaagaaca atagcagatt tagaagaaag agatttgata aattcacagt
     2221 gtattataga agctttttca tttagaaagg cttattatag ttattttaaa tagttttttg
     2281 aggtggattt tatggaaaaa agagatactt atttatggtt aaaatcaatt ggtggaataa
     2341 caacaaaaac aattgaaata atagaaaatg aaatagtaaa tattgaagat atatttgatt
     2401 tttctgaaaa agaaatatat aatttaaaaa atataagctt gaatattaga aaaaatatag
     2461 taaaatatag aggtcacgct tatttagaaa atataaaaga actactttac aaaaaagcga
     2521 ttaaatatat ttgtaaatat gataaagaat atcctgaaaa tttaaagaat atatacaacg
     2581 ctccaaagtt attattctac aaaggtgaca taggcttagt taataataat tttaatatag
     2641 ctatagtagg ttcaagaaaa cctacagcct atggaattaa ttgtgcaaaa acaataagtt
     2701 gtcaactatc ccaatatgga gttaatatag taagtggatt agctatagga atagatgcat
     2761 actcgcacat aggttgtatg agtggaaaat ctaaaactat agcagtactt ggttcaggag
     2821 tagacaatcc tcttccaaaa caaaatttac atttatcaaa taaaatatta gaaaatgggg
     2881 gattattatt atcagagtat aatattaact cgacagtagc cccgtaccat ttttctaata
     2941 gaaatagaat tatcagtggt ttaagtgatg gagtagttgt tgtagaagca gcaataaaaa
     3001 gtggtgcact aatcacagta gattttgctc ttgaacatgg aaaaaatgtt tttgcaatac
     3061 ctggtaatat aaattctcaa atgagtagag gttgtcataa aattataaaa gaaggtgcaa
     3121 aattaattga aaatatagat gatattttaa atgaatataa catatttaat attattgata
     3181 aaaaaataaa tcaaaaatat gataatataa gtctaaatgc caagtctaag caaataattg
     3241 aagctataaa aagagaagga aacttgcata tagatagtat ttgtgattat actggtatag
     3301 aaataaagta tgtaaattca ataatcaatg aattagtact aaatgagtta gtagtagaaa
     3361 tgaataataa aacgtacagt ttaaatgtat aaattaagat taggtagtgg gggtgtatgg
     3421 ttttatggcc aaaacattag tcatcgtaga atcgcctgca aaagctaaaa ctatagaaaa
     3481 atttttaggg aaaagtcatt acacagttaa agcatcagtt ggtcatgtca gagatttacc
     3541 taaaagtaaa ttaggtgtag atatagaaaa taattttgaa cctcaatata taaatataag
     3601 aggaaaaggc gatgtaataa aagaattaaa aaaagaagct aaaaaatcta aacaagtata
     3661 tctagcaact gaccctgata gagaaggtga agctatatca tggcatttag ctcatatact
     3721 aaatttagat gaatctgagg attgtagaat agagtttaat gagataacta aggatgcaat
     3781 taaaaaagca attaaacatc ctagaaatat tgatataaat ttggttgatg cacaacaggc
     3841 aagaagagtg ctagacagac ttttaggtta tcaaataagt cctatattat ggcaaaaggt
     3901 tagaaaaggt cttagtgcag gtagagtgca atctgttaca acaaagttaa tttgtgacag
     3961 agaaaaagaa ataaaggcat ttattccaaa agaatattgg acaatagatg ttgaggctaa
     4021 gacagtaaaa gacaaagata taactttgaa attttatggt aaaaatgatg aaaaaattga
     4081 attagaaaat gaagaagctg taaatgaaat actaaaagaa atggaaggta aagatttaaa
     4141 ggttgagaag atagaatcta aatcaagacg taaatcagct ccaaaaccat ttacaacaag
     4201 tatgcttcaa caagaaggag caaataagtt attttttact actaaaaaaa caatgatgat
     4261 agcacaagaa ttatatgaag gtatcgacat agaaggtgaa ggtactgttg gtcttatatc
     4321 atatataaga actgactcta aaagaatatc tgatgaagca aaagaaaaag ctaagggcta
     4381 tatcgtaaac aatttaggag aaaactacta taaaaacact ggtgataaaa aagaaaaaaa
     4441 ggatactaaa aaggttcaag atgcccatga ggcaataaga ccaacctcag ttgatagaac
     4501 tcctgagagt ataaaagcct cattgagcaa agaccagtac aagttataca atttaatttg
     4561 gagaaggttt gtagctagtc aaatggaaga ttcagtgttt gatatactaa atgtagaatg
     4621 taaaataggc gatattgtat ttaaggcaac tggttcaaaa atgaaatttg atggttatac
     4681 caaggtatat aatttttcag aaagagaaga caaaatttta ccaagtatag aaaaaggaga
     4741 tatattaaat atagaaaata tattgccaga ccaacatttt acacaaccac cagcaagata
     4801 tacagaagct agtttagtaa aaacactaga agaactaggt ataggtagac caagtacgta
     4861 tgctccaaca atagctacta tactgaatag agagtatgtt gaaaagcaag gaacaagtct
     4921 atgtccaaca gaacttggaa taatagttac tgatattttg gaaaataatt tccataaatt
     4981 tatagatgta gattttactg ctgatatgga aagtaaactt gatgttatag aagaaggcaa
     5041 tacagattgg aaaaagattg tatcagaatc atatgcacca cttaaagagg ctattgaaga
     5101 agctcttgaa aatatagaaa aagtaaatat ggatgaagaa actgatgaga tttgcgagaa
     5161 ctgtggttct aatatggtta tcaaatatgg tagatttgga aagtttatgg cttgtaaaaa
     5221 ctatcccgat tgtaaaacaa ctagaccaat tatgaataaa attggtgtta aatgtccaaa
     5281 atgtgaagaa ggagatatta tacttagaaa atctaaaaga ggtaaagctt tttatgggtg
     5341 ttctaattat ccaaactgtg actttgtagc ttggaataag ccaacaggag agttttgtga
     5401 gaagtgtggt tcatatatgg ttgaaaaagt gactaaatct gaaacaaaaa cagtgtgttc
     5461 taataaagaa tgtaataaag agacaaaaga gcataatgac ggaaaagagt gaaaattgaa
     5521 aaaaatcaca taattctctt gaaaaaggtt ccaatctatg ataatctttc aagtgtaaac
     5581 aactaataga aaaataaatt ttcattaaat gtgaaaataa tattagaaaa tataaaaggt
     5641 tattaaaagg cctaaatttc atgaggagat gattaaatgg caagtgaagt gttacaaaaa
     5701 acaaggaaaa taaataaaac attacaaaca agtggtggaa gcagtgtctc ttttgattta
     5761 ctggccggag cattgggcga cgttttaagt tctaatgttt atgtagtaag tgcaaaaggt
     5821 aaagtactag gtcttcattt aaatgatgtt caagacagtt cagttataga agatgagtat
     5881 actaagcaaa agaaattttc agatgaatat actcaaaatg tgttaaaaat tgatgaaaca
     5941 ttagaaaatt taaatggtga gaagatatta gaaatctttc ctgaagaaca tggaagatta
     6001 caaaaatata ctacagtagt tccaatatta ggaagcggtc aaagattagg aacattggta
     6061 ctttcaagat attcaaattc attcaatgat gatgatttag taatagctga atacagtgca
     6121 actgttgttg gtcttgaaat attaagagca ataggtgaag aattagaaga agaaatgaga
     6181 aagaaagctg tagttcaaat ggcaataggc actctgtcct actccgagct tgaagcagtt
     6241 gaacatattt ttgctgaatt ggatggaaaa gaaggtctac ttgtagcaag taagatagct
     6301 gatagagttg gtataactag gtctgtaata gtaaatgcac ttagaaaatt tgagagtgca
     6361 ggtgtgatag aatcaagatc attaggtatg aaaggtactc atataagaat acttaatgac
     6421 aaacttacag atgaattaaa aaaattaaaa aacaatcaat aatctatatt ttataggttt
     6481 agattagaaa aggtatctat ttgaaataga tacctttttt agacgaatta ttaaggtaat
     6541 gataagtata ttaaaaagga gtgagaaaat gcctttagca gataaaataa gaccaaaaac
     6601 aatgaatgat gtaataggtc aaagtcatat aataggtaat ggaaaaatat tgtctaagat
     6661 attacaatct aattttttac caaatatgat attttttggt cctcctggtg ttggtaaaac
     6721 tacagtagcg gaaataatag cagaaagaag caataaaagc ttctataaaa taaatgctac
     6781 taattcatct ttagaagata taaaaaaggt gatatcagag cttggaagta taaataatgt
     6841 aaatggagtc ctattatata tagatgaaat acaaagtttt aataaaaaac agcaacaatc
     6901 aatattagaa tttatggaaa atgggagtat aactttgatt gctagcacta cagaaaatcc
     6961 atatcattat gtatataaag ctcttttaag taggtctact gtatttgaat ttaaaccttt
     7021 agaaaaactt gatgtagaaa aaggactgaa aagagctgtt gaagtattaa atgaagatag
     7081 ctatatggat attgagtgta ataatgatgc tattgaagat atagcaattc tttctgatgg
     7141 agatatgaga agagccttaa atattttgga agtggtagtc tattcaacta aaccaaataa
     7201 ggataatata acttatatag attcagatgt aataagggca tctacattta ataaaattat
     7261 aaactatgac aaaaatggtg atagtcatta tgatatttta agtgcatttc aaaaatctat
     7321 aagaggaagt gacccacagg catctataca ctatctagca agacttataa aaggtgggga
     7381 tttgataagt atatgtagga gacttttagt aatagctagt gaagatatag gtttagctta
     7441 tccaaatgct attgtaattg taaaggcatg tgtagactca gctatgcaac ttggatttcc
     7501 agaagcgaaa ataccattag cagaagctac tattcttttg gctacatctc caaagtctaa
     7561 ttctgcatgt atagcaataa tgaaagctat ggatgaactg gataaagagt ttgttaagga
     7621 tataccaaat agtataaagg atgctcacta ttctggttct aaagaaatag gtaggggttg
     7681 tggatacaaa tatcctcata attttaaaaa tcattatgta aagcaacagt atttaccaga
     7741 tagcataaaa gatagtattt attacatacc acaagaaaat aaaactgaga aaaatataaa
     7801 aaaatattta gaatatctaa atagtgattt ttagactgaa ctttatgtat cgggggtaga
     7861 tattatgatt ataaaactta actcaactta tcacagtaga gttatgaaat acttaaaaaa
     7921 agaacctgag tacaatttgt ttataatagg ggatattgaa agatatggat atggaaataa
     7981 ttttttaaat atatgggcag atgtgggtga acatggtgaa ataaaggcaa tattacttaa
     8041 atattttgaa tttatgatgt tttattctga tggagagtat gatgttgaag gtttttataa
     8101 tctacttcgt aatactaatt acgaagaaat atctgggaaa atatgtgctg tagatgcttt
     8161 agcaaagaga ttaggactta ataatttaaa ggtagttgat ttttgcaaac ttcaaactaa
     8221 aaagtttttg atagataata attgtaatgc aaaggttaag agaattaggc ttggaaatct
     8281 aaaaaagaca gtgaaacttt atgacttaat agatgaattt cacagtacaa ctttagagaa
     8341 tttaaaaaat ggactaagga caggtagagg atattgtata gagattaaca aacaggtggt
     8401 gtcaatggca aagagtactt cagaaaatag gactcatgca atgataattg gagtaggaac
     8461 acatcctaaa tatagagcta aaggacttgc tacaaaatgc ctaattaaac tatgttcaga
     8521 gttactgaga gaaaacaaaa ttccttgttt gttttatgat aatgaagagg ctggaaagat
     8581 atataaaaaa ctaggatttg aaaacattgg aaaatgggga atttattcaa aataaaaaat
     8641 taaaattaat cttgacaaaa ttagtagggt ataataaaat tatcttgaaa gtaattccta
     8701 ctaaaacagt atgaattaga tttctatata gaggtgataa tatatgaagt tgtctactaa
     8761 aggtagatat ggactaaaag caatgtttga attagcatta aatcaagaca atggaccagt
     8821 atcattaaaa tttattgcta agaagcaaaa aatatcagat cagtacttag aacaaatatt
     8881 ttcatcttta aaaaaatcgg gtctagtaaa aagtgtgaga ggtgctcaag gaggttattt
     8941 gctttcaaag aatgctgaag acataacagt aggagatata cttgtagtat tagaaggacc
     9001 agttgcatta tctgactgtg tactagatga agatgtatgt gaaaactcaa acatgtgtgt
     9061 aacaaaaata gtttgggaaa agatgaaaaa gggtatagaa gatgttatag attcaattac
     9121 tcttaaagat atgataaatg actataacaa aaataaatta gaaaatgata taacaaatat
     9181 taaaaaatag atttggagat gaatgtaatg gaaaaaagaa gattgtatat ggattattct
     9241 gcaacaacac ctattaaaaa agaagtattg gatgcaatga tgccatacct aacagattat
     9301 tttggtaatg catctagttt tcacacattc ggaagagaag caaaagatgc tttggataaa
     9361 gcaagagaac aagtagcagc ccttataaat gctgaaccta gtgaaatata ttttacagca
     9421 ggtggttcag aaagtgataa ctggacacta gagggtatag cttatgctaa taaaaacaaa
     9481 ggtaatcata taataacttc aaaaatagaa caccatgcaa tacttcatac atgtgaatac
     9541 ttagcaaaac atcatggatt tgaaataact tatttagatg ttgatagcga aggaaaagta
     9601 gacttaaagc aactagaaga ttcaataaaa gatactacta tacttataag tataatgttt
     9661 gcaaataatg aaataggaac tatacagcct attaaagaaa tatccgaaat agctaaaaaa
     9721 cataagatat tattccacac tgatgcagta caagctacag gtaatattcc agtagatgtt
     9781 aaagaattag gaatagattt gatgagtatg tcttcacata aaatatatgg accaaaaggt
     9841 gtaggagctt tatacataag aaaaggtgta agacttcata actttgtcca tggtggagca
     9901 caagaaaaaa gcaaaagagc tggtacagaa aatatacctg caatagttgg atatggaaaa
     9961 gcagcagaat tagctaagga aaatatgcaa aatcatgtag aaactttaac tcgtcttaga
    10021 aataagttaa tagatggtgt gttagaaaga ataccttaca caagagtaaa tggtagctta
    10081 gaaaatagat tgccaggaaa tgcaaacttt gcattccaat ttatagaagg tgaaggaata
    10141 cttttattat tagatatgtt aggaattgct ggttcaagtg gttctgcatg tacttctggt
    10201 tcattagacc cttcacatgt acttcttgca ataggtttac cacatgaaat agcacatggt
    10261 tcattaagac ttacagttgg agattttaca acagatgatg atatagatta tatactagag
    10321 aacttaccaa aagtaataga aagacttaga agcatgtcac cactttatga tgatgcgaag
    10381 aaacaaggtt tagtaaaata gacataaaga taaagcataa aaaatagaca taaattgagt
    10441 aggaggaaaa gataatgcaa tatagtgata aagttatgga acattttatg aatccaagaa
    10501 atatgggaga aattgataat gcaagtggtg taggtgaagt tggaaaccct acatgtggag
    10561 atataatgaa gatattttta gacatagatg gagatgtaat aaaggatgtt aagtttaaaa
    10621 catttggctg tggttcagct atagcaagtt cttctatggc aactgaaatg ataaaaggaa
    10681 aaactataaa agatgcatta gaacttacaa ataaagctgt tgcagaagct ttggatggtt
    10741 taccaccagt aaagatgcat tgttcagttt tagctgaaca agctgtaaaa gcagctctta
    10801 tagattatgc tcaaaagaac aatatccata taccagaatt ggatggatat gttatagatg
    10861 atgctcatga ccatgatgtt gaagaagaag aataaaaata agtgattgtg ttatgagaag
    10921 tttacaatga ttaaaaagaa gtactaatga tataattagg acataaaaaa tagctgtttt
    10981 taaaatagct attttttatt gcctaaatta agtattttaa cttgatttag aggttaatta
    11041 gttaacaaaa taatccataa gattcaaata ataaagatga ttattttttt agaaaataga
    11101 acaagctata ttttaaataa gtacaatact tgctctatat ggtataataa taagtaatgc
    11161 aaaagatgag gtgaaatttg tttatgaata aaaaagtaat gataggtatg agtggaggag
    11221 ttgatagctc tgtagctgca tatcttttaa aacaacaagg atatgatgtc attggtgtta
    11281 ctatgaagtt gtggcaagat gatgatgttg ttgaaattga aggtggttgt tgctctttat
    11341 ctgctgttga agatgctaga agggtagcaa ataaaatagg tataccattt tatgttttaa
    11401 attttagaga agtgtttaaa gaaaaagtaa tagattattt tatagatgaa tatttggaag
    11461 gaaaaactcc taatccctgt atagcttgta ataaacatat aaaatttgat gatttttata
    11521 agaaggctag acaaataggt tgtgattatg tagctacagg tcactatgca aagatagaaa
    11581 aggatgaaag tacaggaaga tatttgctaa aaaaatctgt tacagataaa aaagaccaaa
    11641 cttatgcact ttataatctt acacaagagc aactagagca tactttattg ccaataggtg
    11701 actatgaaaa agatagagta agagaaattg ctaaggaaat gggtatggca gtacacaata
    11761 agcctgatag tcaagaaatt tgttttgtta aagataatga ctatgcaaat tatgtaaaaa
    11821 aacattctaa aaagcgtatt gaagaaggtt tttttgtaga tactaaaggc aatatacttg
    11881 gaaaacataa gggtatatta tattatacta taggccaaag aaaagggctt ggaattactt
    11941 ttggaaaacc aatgtttgta atagacataa atccaataaa taataccata gttcttggag
    12001 acaatgaaga tttatttaaa aaggaactta ttgctaaaga tgttaacttt atatcaatag
    12061 atacgttaga agaaccctta agagttcaag ccaaaataag atattcagca aaaccatcac
    12121 cagctactat tcatagagta ggagaagata ctattaaaat agtttttgat gaagctcaaa
    12181 gagctattac taaaggtcaa tctgtagtaa tgtatgatgg agatattgta gtaggtggag
    12241 gtataatcga gaaaagttta taaaaacatc taaaaaatta aaaaaataag caataaagta
    12301 tattaagagt aaataaagtt tataaaataa atttattgta tatgaaaata tagtattgat
    12361 taattttgta aaatttagta atataattag tatattaatt aaaaaacaaa tacagtgata
    12421 aggaatagta aaatatttga tgtcttacag agagagggaa atgctggaag cccttagaat
    12481 atgatatttg aagcagcctt ttagtagcaa tcttgaacaa tttatctttt ataaagaaga
    12541 tttaagtaga gattgacggt agttacgtta tagctaaggt tttccgaaga tatcatactt
    12601 tgtatgataa ttagggtggt accgcgattg tcctcgcccc tataaattta tttataggga
    12661 cgaggttttt tatttaaacg aaaaaataaa atttattagg aggaacatga tatggaaaag
    12721 atgggattaa atgaaataag aagtaagttt ttagaatttt ttgagtcaaa aggtcattat
    12781 gtagcaaata gttattcttt agtaccaaac aatgataaga gtttgttact tataaactca
    12841 ggtatggcac ctttgaaaaa ctacttttca ggtgtagaag tacctccaag tgtgagaatg
    12901 tgtacatctc aaaaatgtat aagaacagga gatatagaaa atgtaggtat aactgcaaga
    12961 catgctacat tctttgaaat gatgggtaat ttctcatttg gagattactt taaaagagag
    13021 tcaataaaat ggggatggga atttgttaca gaatggctaa atataccaga agataagata
    13081 tgggtgactg tatatgaaga agatgatgat tcttatgata tatgggcaaa agaaatgaat
    13141 ttcccagaag aaagaatggt tagacttgga aaagatgata atttctggga aataggtaca
    13201 ggtccttgtg gtccttgttc agaaatatat tttgatagag gagaagaata tggatgtgac
    13261 aatccagact gtaaaccagg ctgtgattgt gataggtatt tagaattttg gaaccatgta
    13321 tttactcaat ttgatagaga tgaagaagga aattatagtc tattagaaaa caagaatata
    13381 gatacaggta tgggtcttga aagaatggga tgtattatgc aaggcgtaga cactatcttt
    13441 gaagttgata caattaaatc tatactagaa gcagttgaga agttaacagg agtaaaatat
    13501 ggagagaatc caaaaaatga tatatcaata agaataataa ctgaccatat aagagctgta
    13561 acattcttag ttagtgatgg agtgcttcca tcaaatgaag gaagaggata tgttcttaga
    13621 agactcctta gacgtgctgc tcgtcatggt aagttattag gagtaaaaga attattttta
    13681 cagaaactaa tagatgaagt tataaaagta aatgataaag catatccagt tttagttgaa
    13741 aaagaaagtt atattaaaaa agtagttgga atagaagaag aaaaattcaa tgaaactata
    13801 gaccaaggaa cagaaatatt gaactcttat atagaagtat taaaaaatga aggaaagact
    13861 gtcctaagtg gtcaagaagc ttttaaacta tatgatactt atggcttccc tatagactta
    13921 actaaagaaa tattagaaga agaacatcta tctgtagatg aagaagcttt taatgaagaa
    13981 atggaaaagc aaaaagaaag agctagaaat gctagaggaa atatggatgg agaaagttgg
    14041 aaggaagacc ctctatcaaa attagaatct acagttgaca gtacatttaa tgggtatagt
    14101 gaaatttatg gtgaaggaac tattgaagct atagttaaag atgatgagct agttcaaagt
    14161 gcagaagaag gcgataaggt atctatagta ctagataaca ctactttcta tcctgaaggt
    14221 ggaggacaag ttggagattg tggattgata actaatgaaa acttagtttt agaagtatta
    14281 aatactaaaa aaggtgctaa taatagtata aaacatatag gtataataaa atcaggaaga
    14341 ataagtaatg gggacaaagt gaagacactc gtagatagag aaactagaat gtcagctgca
    14401 agaaatcaca gtgctacaca tttacttcat aaggcattaa gagaagtatt aggagaacat
    14461 gttaatcaag ctggttcttt agtaactcca gaaagactta gatttgatat tactcacttt
    14521 gaagcgatat caaatgaaga attaaaagta atagaagaaa aagtaaataa tgttatttta
    14581 tcatctttag acataaaatg tgatattatg aatataaagg aagctaaaga aaaaggagct
    14641 acagcactat ttggtgaaaa atatggtgat gaggtaagag tagtttctat gggagattat
    14701 tctacagaac tttgtggagg aactcacttg acaaatactt ctcaagtagg aatgttcaaa
    14761 atattatctg aaggtggagt agcagcaggt gtaagaagaa tagaagcaat aacaggaaaa
    14821 gctgtttatg aatacctaaa agaaagagat ggaattatct ctgaagtttg tgtaaactta
    14881 aaatctaaag aagataattt aattcaaaga ataagttctt tattagaaga aaataaaaac
    14941 ttatctaaag aattgcatga tatgaaagct aagatgagtt tacaatcagt tgattctatt
    15001 tttgattcta aagttgaagt aaatggagtt aatttgataa ctaataaatt tgaaggtatg
    15061 gatatggata cactaagaga aacagcagat aatctaagag ataagcttgg ttctggtgta
    15121 gtagtacttg caaatgtagt agatgataaa gttaactttg tagttactgc cactaaagat
    15181 gtattagata agggaataca ttcaggtaat attgttagag aagttgcaaa aatagctgga
    15241 ggaaaaggtg gaggaagacc taatatggca caagcaggag ctagtgatgt atctaaggtt
    15301 gaccaagctt taagctatgc aagtgaggtt attaagacac aagttaaata acattcactc
    15361 aaggaggttt ttagtttgga aaaagattta gactacacta tgaaatttga aggaatacca
    15421 gaagatagga tgagtgtagg agatactatt gattttgtat ataaagcatt agttgaaaaa
    15481 ggatataacc ctataaatca aattataggg tatctccttt ctggtgattc tagctatata
    15541 actagtcata aaaatgcaag agctataatt aaaaaatttg aaagagatga gatacttgaa
    15601 gaagtaatca cgcactatct gaatagaaag taggtcaaat aaatgagaaa aaagattatg
    15661 tcgttgtttg caatagtcat tatgattttt gtatcaactg ctagctatgg ttttgctgaa
    15721 gaaaaaggca aggtcatatt tattgatatg aataggacca atcttggaag tatgatgaat
    15781 attccaatct tgaaaaatga gatagaaaag agaggctatg tagctctcat gaatataaga
    15841 ggggatcaag gtactgatga tagaaggtct tatgcaagta tgggtgcagg aacaagagct
    15901 agtgtaacta ctgaagacta tatcaatttt caaaaagtag accaagatac tgctaaagct
    15961 tttgaagtcg caacaggtca aaaagctaaa gaaataaatg atttaggcat taatagatct
    16021 ataaatgaaa atcttgagta tggagaatat ggagcaaatt tagggtcatt aggaaaaact
    16081 ttatcagaaa atggtttaaa gacatctgta attggaaatg ctgatataat tgaaaataat
    16141 caattagtta aaaacagaaa cttatgttta gtagctatgg accaatatgg aaggattgat
    16201 tcaggtaata ttgataatat taataagaaa gattctaaaa tgccatttgg aatatctaca
    16261 gattatgata aattactaga tgaaactaaa aaatcctata aaaatagtga tgctatattt
    16321 gtagaacttg gagatacata tagattagat atgtatagat taaatctaaa tgataatact
    16381 tatgatataa tgaagaaaaa tattgtaaaa aatataaata aatatcttga acaagtattt
    16441 tctatggttg gagaaaatga cactgtttat atagcaagtg catttccaag tgacctagat
    16501 tataagaata agagaagact ttctccaata ataaaattta gtggagaagg gaaaggtcta
    16561 ctggaatctg ccacaacaag aaaggctgga attgttgcca atttagatgt tggagtagat
    16621 atactaggta catttggtct tgaaaataaa gagatggtag gtagaagtta tactttagta
    16681 aataaagagg acaatattag ctatctaaaa tcactttatg aaaggatagt gtctatatct
    16741 tcaataaggt caactattat aaatggcttt gtaggcgtaa tatctgcttc ttgggtaatt
    16801 gccatgtttg cgattttctt tagaaattat atgccacata aagagcgtgt attcaaagta
    16861 cttaaggagt ttataaaatt aggcataata atgccactat catttatgtt agctcctttg
    16921 atgaatttta aaactccaat ttcaatatct ttgggaatat ttgcaactac gctaatatta
    16981 tacttgttag gtagaaaatt atttaaggac aatgatataa agcacatggg attttttgca
    17041 acacttacta ttttagtcat agttatagat tctgtatttg gaacatactt aatgcaaaat
    17101 aacataatga gttatgatgc tgtcatagga gctaggtact atggtgtagg aaatgaatat
    17161 gaaggtgtaa ctatagcaag tgcagtattt ggcttagcag tattattaca ttataagaag
    17221 gtatctaaat tacttgttgt aattttttca ttaataatac ttataactag tgcatatcct
    17281 tcaatgggtg caaatgttgg tggagctata tcagaatgtg tagcatattt gttgtttata
    17341 atgcttatat atgacattaa aatagatttt aagaaagctg tattattagc tgtttcagct
    17401 gtagttgtcg tttttgcatt tgcagcttta gatatacttt caggtagtga atctcattta
    17461 ggtatgtttg tacagagaat tttcctaaat ggaccaggag agatattaca aacatttgga
    17521 agaaaaattc aaatgaatat gcaactagca cagacaagtg tatgggtaaa tattttactt
    17581 gtaggaattg gagtaattgc agtgcttata tttaaaccat ctagacactt tagaaagata
    17641 atgaataatt ttccaatctt gtttaaaggg tttattgcat ctatggttgg ttgtataatt
    17701 actcttttag ttaatgattc tggaatagtt gctgcatcta cagcatctat atatatactt
    17761 ataccactaa taataataag tataaatatg ataatatttg aaaaaaataa ctaaaaaaat
    17821 ttttactgtt catataggat ttaaataaaa aatgcaatat gattattaat gtaaactgga
    17881 tgcttacata aaaaatcata ttgcaatttt tatgattgat tatgtaaaat tatatgaagc
    17941 agtaagcctg tgaccttagt tatgataggt tcagaatatg tatttaatct atagaatata
    18001 aatagataaa ggaggaacta tgttagatgg aagaataatg ggtcttgatg ttggtgataa
    18061 aactattgga gttgctgtga gtgatttgat gggattaact gcacaaggtg taaaaacaat
    18121 aaaaagagtt ggcaagaaaa aagatattga agagctaaaa gcaataataa aagaaaaaca
    18181 agtaaataaa attgtatcag gattaccaaa aaatatgaat ggaactttag gtccacaagg
    18241 agaaaaagta atcaagtttt gtgaattggt aaaagcagaa acaggtattg atgtagagtt
    18301 ttgggatgag aggctttcta cagtagcggc agagaggtct ttattagaag cagatgttag
    18361 tagacaaaaa agaaaaaagg ttatagatat gttggcagca gtaataattt tgcaaggata
    18421 tttagatttt aaaataaatt cataaaagaa ctataaaatt gaatacttat gatatataat
    18481 ataaataata aaaataaaaa taaatttgag gtgaatgaca ttatggaaga aaatataata
    18541 aatttaatag acgaaaatgg cgttgaaagc caatttgaaa taatattaac attagaagca
    18601 gaaggaaaag agtatgcaat attaatgcct ttagatgatg aagaagctga agaagcttta
    18661 atctttagaa ttgatgagga tgaagaaggt gaaatcttaa tacctttgga aagtgatgaa
    18721 gagtatgaaa ctgttgtagc tgtgtatact gctataatgg aagaagaagg tttaaattac
    18781 gacgaagatg aatcaaatgg acttaactag ataagtaatt aagtttgtat ataaaaatca
    18841 gatatttatt atatttgctt taattaaacc ttattaattg taaaagcatt tatagtatat
    18901 atctgttttt ttgtttttta ttaaaaatac caaaaaattt atgaattttg gtgaa
//
LOCUS       DAMJYC010000079        18772 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJYC010000079 DAMJYC010000000
VERSION     DAMJYC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.080"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            165..719
                     /gene="tcdR"
                     /locus_tag="PC472_002891"
     CDS             165..719
                     /gene="tcdR"
                     /locus_tag="PC472_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2728200.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1037..8137
                     /gene="tcdB"
                     /locus_tag="PC472_002892"
                     /gene_synonym="toxB"
     CDS             1037..8137
                     /gene="tcdB"
                     /locus_tag="PC472_002892"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2728201.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8259..8759
                     /gene="tcdE"
                     /locus_tag="PC472_002893"
     CDS             8259..8759
                     /gene="tcdE"
                     /locus_tag="PC472_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2728202.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8838..8999
                     /locus_tag="PC472_002894"
     CDS             8838..8999
                     /locus_tag="PC472_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728203.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9088..9270
                     /locus_tag="PC472_002895"
     CDS             9088..9270
                     /locus_tag="PC472_002895"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728204.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9487..17619
                     /gene="tcdA"
                     /locus_tag="PC472_002896"
                     /gene_synonym="toxA"
     CDS             9487..17619
                     /gene="tcdA"
                     /locus_tag="PC472_002896"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2728205.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17954..18652)
                     /gene="tcdC"
                     /locus_tag="PC472_002897"
     CDS             complement(17954..18652)
                     /gene="tcdC"
                     /locus_tag="PC472_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2728206.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPSCSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7419 a         2074 c         2904 g         6375 t
ORIGIN      
        1 aaaagattac taattaatta ttaaaattaa tgtattcata atgcatattt tcatataaaa
       61 tttaatttat ttgccgatta tataattata atgactgatt taattccgat gttgtcaaaa
      121 ttttcaaata aatcatcatt ataaatataa gagaggatga ttttatgcaa aagtcttttt
      181 atgaattaat tgttttagca agaaataact cagtagatga tttgcaagaa attttattta
      241 tgtttaagcc attagtaaaa aaacttagta gagttttaca ttatgaagag ggagaaacag
      301 atttaataat attttttatt gaattaataa aaaatattaa attaagtagc ttttcagaaa
      361 aaagcgatgc tattatagtc aaatatattc ataaatcatt actgaataag acttttgagt
      421 tgtctagaag atattctaaa atgaagttta attttgtaga atttgatgaa aatatcttaa
      481 atatgaaaaa taattatcaa agtaagtctg tttttgagga agatatttgt tttttcgaat
      541 atattttgaa agaattatct ggtattcaaa gaaaagttat tttttataaa tatttaaaag
      601 gatattctga tagagaaata tcagtgaaat taaaaatatc tagacaagct gttaataagg
      661 ctaaaaatag agcatttaaa aaaataaaaa aagactatga aaattatttt aacttgtaat
      721 taatgagctt aaagaaatat ttacaataga aatcaaattt tagaattaac tttattgtaa
      781 aatcaataac ttaatctaag aatatcttaa tttttatatt ttatatagaa caaagtttac
      841 atatttattt cagacaacgt ctttattcaa tcgaagagca aattaatcaa ctgagtgtct
      901 taaatttaaa atgttaggaa gtgaatgtat atgaaaacct aagtagatat tagtatattt
      961 tataaataga aaggaggata tataaaagag ttttagcatt tagatgtaaa aatattatag
     1021 taaaggagaa aattttatga gtttagttaa tagaaaacag ttagaaaaaa tggcaaatgt
     1081 aagatttcgt actcaagaag atgaatatgt tgcaatattg gatgctttag aagaatatca
     1141 taatatgtca gagaatactg tagtcgaaaa atatttaaaa ttaaaagata taaatagttt
     1201 aacagatatt tatatagata catataaaaa atctggtaga aataaagcct taaaaaaatt
     1261 taaggaatat ctagttacag aagtattaga gctaaagaat aataatttaa ctccagttga
     1321 gaaaaattta cattttgttt ggattggagg tcaaataaat gacactgcta ttaattatat
     1381 aaatcaatgg aaagatgtaa atagtgatta taatgttaat gttttttatg atagtaatgc
     1441 atttttgata aacacattga aaaaaactgt agtagaatca gcaataaatg atacacttga
     1501 atcatttaga gaaaacttaa atgaccctag atttgactat aataaattct tcagaaaacg
     1561 tatggaaata atttatgata aacagaaaaa tttcataaac tactataaag ctcaaagaga
     1621 agaaaatcct gaacttataa ttgatgatat tgtaaagaca tatctttcaa atgagtattc
     1681 aaaggagata gatgaactta atacctatat tgaagaatcc ttaaataaaa ttacacagaa
     1741 tagtggaaat gatgttagaa actttgaaga atttaaaaat ggagagtcat tcaacttata
     1801 tgaacaagag ttggtagaaa ggtggaattt agctgctgct tctgacatat taagaatatc
     1861 tgcattaaaa gaaattggtg gtatgtattt agatgttgat atgttaccag gaatacaacc
     1921 agacttattt gagtctatag agaaacctag ttcagtaaca gtggattttt gggaaatgac
     1981 aaagttagaa gctataatga aatacaaaga atatatacca gaatatacct cagaacattt
     2041 tgacatgtta gacgaagaag ttcaaagtag ttttgaatct gttctagctt ctaagtcaga
     2101 taaatcagaa atattctcat cacttggtga tatggaggca tcaccactag aagttaaaat
     2161 tgcatttaat agtaagggta ttataaatca agggctaatt tctgtgaaag actcatattg
     2221 tagcaattta atagtaaaac aaatcgagaa tagatataaa atattgaata atagtttaaa
     2281 tccagctatt agcgaggata atgattttaa tactacaacg aataccttta ttgatagtat
     2341 aatggctgaa gctaatgcag ataatggtag atttatgatg gaactaggaa agtatttaag
     2401 agttggtttc ttcccagatg ttaaaactac tattaactta agtggccctg aagcatatgc
     2461 ggcagcttat caagatttat taatgtttaa agaaggcagt atgaatatcc atttgataga
     2521 agctgattta agaaactttg aaatctctaa aactaatatt tctcaatcaa ctgaacaaga
     2581 aatggctagc ttatggtcat ttgacgatgc aagagctaaa gctcaatttg aagaatataa
     2641 aaggaattat tttgaaggtt ctcttggtga agatgataat cttgattttt ctcaaaatat
     2701 agtagttgac aaggagtatc ttttagaaaa aatatcttca ttagcaagaa gttcagagag
     2761 aggatatata cactatattg ttcagttaca aggagataaa attagttatg aagcagcatg
     2821 taacttattt gcaaagactc cttatgatag tgtactattt cagaaaaata tagaagattc
     2881 agaaattgca tattattata atcctggaga tggtgaaata caagaaatag acaagtataa
     2941 aattccaagt ataatttctg atagacctaa gattaaatta acatttattg gtcatggtaa
     3001 agatgaattt aatactgata tatttgcagg ttttgatgta gattcattat ccacagaaat
     3061 agaagcagca atagatttag ctaaagagga tatttctcct aagtcaatag aaataaattt
     3121 attaggatgt aatatgttta gctactctat caacgtagag gagacttatc ctggaaaatt
     3181 attacttaaa gttaaagata aaatatcaga attaatgcca tctataagtc aagactctat
     3241 tatagtaagt gcaaatcaat atgaagttag aataaatagt gaaggaagaa gagaattatt
     3301 ggatcattct ggtgaatgga taaataaaga agaaagtatt ataaaggata tttcatcaaa
     3361 agaatatata tcatttaatc ctaaagaaaa taaaattaca gtaaaatcta aaaatttacc
     3421 tgagctatct acattattac aagaaattag aaataattct aattcaagtg atattgaact
     3481 agaagaaaaa gtaatgttaa cagaatgtga gataaatgtt atttcaaata tagatacgca
     3541 aattgttgag gaaaggattg aagaagctaa gaatttaact tctgactcta ttaattatat
     3601 aaaagatgaa tttaaactaa tagaatctat ttctgatgca ctatgtgact taaaacaaca
     3661 gaatgaatta gaagattctc attttatatc ttttgaggac atatcagaga ctgatgaggg
     3721 atttagtata agatttatta ataaagaaac tggagaatct atatttgtag aaactgaaaa
     3781 aacaatattc tctgaatatg ctaatcatat aactgaagag atttctaaga taaaaggtac
     3841 tatatttgat actgtaaatg gtaagttagt aaaaaaagta aatttagata ctacacacga
     3901 agtaaatact ttaaatgctg cattttttat acaatcatta atagaatata atagttctaa
     3961 agaatctctt agtaatttaa gtgtagcaat gaaagtccaa gtttacgctc aattatttag
     4021 tactggttta aatactatta cagatgcagc caaagttgtt gaattagtat caactgcatt
     4081 agatgaaact atagacttac ttcctacatt atctgaagga ttacctataa ttgcaactat
     4141 tatagatggt gtaagtttag gtgcagcaat caaagagcta agtgaaacga gtgacccatt
     4201 attaagacaa gaaatagaag ctaagatagg tataatggca gtaaatttaa caacagctac
     4261 aactgcaatc attacttcat ctttgggggt agctagtgga tttagtatac ttttagttcc
     4321 tttagcagga atttcagcag gtataccaag cttagtaaac aatgaacttg tacttcgaga
     4381 taaggcaaca aaggttgtag attattttaa acatgtttca ttagttgaaa ctgaaggagt
     4441 atttacttta ttagatgata aaataatgat gccacaagat gatttagtga tatcagaaat
     4501 agattttaat aataattcaa tagttttagg taaatgtgaa atctggagaa tggaaggtgg
     4561 ttcaggtcat actgtaactg atgatataga tcacttcttt tcagcaccat caataacata
     4621 tagagagcca cacttatcta tatatgacgt attggaagta caaaaagaag aacttgattt
     4681 gtcaaaagat ttaatggtat tacctaatgc tccaaataga gtatttgctt gggaaacagg
     4741 atggacacca ggtttaagaa gcttagaaaa tgatggcaca aaactgttag accgtataag
     4801 agataactat gaaggtgagt tttattggag atattttgct tttatagctg atgctttaat
     4861 aacaacatta aaaccaagat atgaagatac taatataaga ataaatttag atagtaatac
     4921 tagaagtttt atagttccaa taataactac agaatatata agagaaaaat tatcatattc
     4981 tttctatggt tcaggaggaa cttatgcatt gtctctttct caatataata tgggtataaa
     5041 tatagaatta agtgaaagtg atgtttggat tatagatgtt gataatgttg tgagagatgt
     5101 aactatagaa tctgataaaa ttaaaaaagg tgatttaata gaaggtattt tatctacact
     5161 aagtattgaa gagaataaaa ttatcttaaa tagccatgag attaattttt ctggtgaggt
     5221 aaatggaagt aatggatttg tttctttaac attttcaatt ttagaaggaa taaatgcaat
     5281 tatagaagtt gatttattat ctaaatcata taaattactt atttctggcg aattaaaaat
     5341 attgatgtta aattcaaatc atattcaaca gaaaatagat tatataggat tcaatagcga
     5401 attacagaaa aatataccat atagctttgt agatagtgaa ggaaaagaga atggttttat
     5461 taatggttca acaaaagaag gtttatttgt atctgaatta cctgatgtag ttcttataag
     5521 taaggtttat atggatgata gtaagccttc atttggatat tatagtaata atttgaaaga
     5581 tgtcaaagtt ataactaaag ataatgttaa tatattaaca ggttattatc ttaaggatga
     5641 tataaaaatc tctctttctt tgactctaca agatgaaaaa actataaagt taaatagtgt
     5701 gcatttagat gaaagtggag tagctgagat tttgaagttc atgaatagaa aaggtaatac
     5761 aaatacttca gattctttaa tgagcttttt agaaagtatg aatataaaaa gtattttcgt
     5821 taatttctta caatctaata ttaagtttat attagatgct aattttataa taagtggtac
     5881 tacttctatt ggccaatttg agtttatttg tgatgaaaat gataatatac aaccatattt
     5941 cattaagttt aatacactag aaactaatta tactttatat gtaggaaata gacaaaatat
     6001 gatagtggaa ccaaattatg atttagatga ttctggagat atatcttcaa ctgttatcaa
     6061 tttctctcaa aagtatcttt atggaataga cagttgtgtt aataaagttg taatttcacc
     6121 aaatatttat acagatgaaa taaatataac gcctgtatat gaaacaaata atacttatcc
     6181 agaagttatt gtattagatg caaattatat aaatgaaaaa ataaatgtta atatcaatga
     6241 tctatctata cgatatgtat ggagtaatga tggtaatgat tttattctta tgtcaactag
     6301 tgaagaaaat aaggtgtcac aagttaaaat aagattcgtt aatgttttta aagataagac
     6361 tttggcaaat aagctatctt ttaactttag tgataaacaa gatgtacctg taagtgaaat
     6421 aatcttatca tttacacctt catattatga ggatggattg attggctatg atttgggtct
     6481 agtttcttta tataatgaga aattttatat taataacttt ggaatgatgg tatctggatt
     6541 aatatatatt aatgattcat tatattattt taaaccacca gtaaataatt tgataactgg
     6601 atttgtgact gtaggcgatg ataaatacta ctttaatcca attaatggtg gagctgcttc
     6661 aattggagag acaataattg atgacaaaaa ttattatttc aaccaaagtg gagtgttaca
     6721 aacaggtgta tttagtacag aagatggatt taaatatttt gccccagcta atacacttga
     6781 tgaaaaccta gaaggagaag caattgattt tactggaaaa ttaattattg acgaaaatat
     6841 ttattatttt gatgataatt atagaggagc tgtagaatgg aaagaattag atggtgaaat
     6901 gcactatttt agcccagaaa caggtaaagc ttttaaaggt ctaaatcaaa taggtgatta
     6961 taaatactat ttcaattctg atggagttat gcaaaaagga tttgttagta taaatgataa
     7021 taaacactat tttgatgatt ctggtgttat gaaagtaggt tacactgaaa tagatggcaa
     7081 gcatttctac tttgctgaaa acggagaaat gcaaatagga gtatttaata cagaagatgg
     7141 atttaaatat tttgctcatc ataatgaaga tttaggaaat gaagaaggtg aagaaatctc
     7201 atattctggt atattaaatt tcaataataa aatttactat tttgatgatt catttacagc
     7261 tgtagttgga tggaaagatt tagaggatgg ttcaaagtat tattttgatg aagatacagc
     7321 agaagcatat ataggtttgt cattaataaa tgatggtcaa tattatttta atgatgatgg
     7381 aattatgcaa gttggatttg tcactataaa tgataaagtc ttctacttct ctgactctgg
     7441 aattatagaa tctggagtac aaaacataga tgacaattat ttctatatag atgataatgg
     7501 tatagttcaa attggtgtat ttgatacttc agatggatat aaatattttg cacctgctaa
     7561 tactgtaaat gataatattt acggacaagc agttgaatat agtggtttag ttagagttgg
     7621 ggaagatgta tattattttg gagaaacata tacaattgag actggatgga tatatgatat
     7681 ggaaaatgaa agtgataaat attatttcaa tccagaaact aaaaaagcat gcaaaggtat
     7741 taatttaatt gatgatataa aatattattt tgatgagaag ggcataatga gaacgggtct
     7801 tatatcattt gaaaataata attattactt taatgagaat ggtgaaatgc aatttggtta
     7861 tataaatata gaagataaga tgttctattt tggtgaagat ggtgtcatgc agattggagt
     7921 atttaataca ccagatggat ttaaatactt tgcacatcaa aatactttgg atgagaattt
     7981 tgagggagaa tcaataaact atactggttg gttagattta gatgaaaaga gatattattt
     8041 tacagatgaa tatattgcag caactggttc agttattatt gatggtgagg agtattattt
     8101 tgatcctgat acagctcaat tagtgattag tgaatagata aaaatatgtt aaatatatcc
     8161 tcttatactt aaatatataa aaataaacaa aatgatacac tacataaagt gttctatcta
     8221 atatgaagat ttaccaataa aaaggtggac tatgatgaat gcacagtagt tcaccttttt
     8281 atatttctaa tggtaacaaa atattttttt atataaacct aggaggcgtt atgaatatga
     8341 caatatcttt tttatcagag catatattta taaagttagt aattttaact atatcatttg
     8401 atacattatt aggatgttta agtgcaataa aaagtcgtaa atttaattct agttttggaa
     8461 tagatggagg aatcagaaaa gtagcaatga tagcatgtat atttttttta tcagtagttg
     8521 acattcttac aaagtttaac tttttattta tgttaccaca agattgtatc aattttttaa
     8581 gactaaaaca tcttggaata tctgaatttt tctctatttt atttatttta tatgaaagtg
     8641 taagtatatt aaaaaatatg tgcttatgtg gattaccagt acctaagaga ttaaaggaaa
     8701 aaatagcaat tttactagat gcaatgacag atgaaatgaa tgctaaggat gaaaagtaag
     8761 taatggtaga tataataaag atattaacaa ataaaaagtg ttatccaaat aagaatagct
     8821 gaaagttatc ataattcatg aaactaataa tgaaaacgag ggagcagatg ccaagagaca
     8881 cacaagtatt aaatacatat aatttcgaag caagtgttca ttactatata gatgacaagg
     8941 tagtatatca aacattggtt cacaaagatg gtgcatggtc agttggtaaa atctattaag
     9001 ctacattagt tacagatatc acaaactata atagttaaac atagaaatat gtgtaaattg
     9061 tgatggaaat tattcaaaaa cacaaaaata cgtgatgaag gacaaaatga tatagaaaat
     9121 aagtatcaaa ccttaataaa tgatttaatt gatagtttaa aagttatagg aaaaatatat
     9181 aaagaaataa aaacattaaa aaaatataag atatgtttac aaattactat cagacaatct
     9241 ccttatctaa tagaagagtc aattaactaa ttgagtatct ttaaattgaa atgttaggaa
     9301 gtgatttaaa tatgaaaact taaattataa aaaatcaata ttaatttatt tttaaaaaat
     9361 agaaaggagt gtataagatt tattttcaaa gtttaaaaac aagaaaatca atttaaattt
     9421 cagaaggaat aaatgtggtt atagaagtgg atttattatc aaaaataata atactaggag
     9481 gtttttatgt ctttaatatc taaagaagag ttaataaaac tcgcatatag cattagacca
     9541 agagaaaatg agtataaaac tatactaact aatttagacg aatataataa gttaactaca
     9601 aacaataatg aaaataaata tttacaatta aaaaaactaa atgaatcaat tgatgttttt
     9661 atgaataaat ataaaacttc aagcagaaat agagcactct ctaatctaaa aaaagatata
     9721 ttaaaagaag taattcttat taaaaattcc aatacaagcc ctgtagaaaa aaatttacat
     9781 tttgtatgga taggtggaga agtcagtgat attgctcttg aatacataaa acaatgggct
     9841 gatattaatg cagaatataa tattaaactg tggtatgata gtgaagcatt cttagtaaat
     9901 acactaaaaa aggctatagt tgaatcttct accactgaag cattacagct actagaggaa
     9961 gagattcaaa atcctcaatt tgataatatg aaattttaca aaaaaaggat ggaatttata
    10021 tatgatagac aaaaaaggtt tataaattat tataaatctc aaatcaataa acctacagta
    10081 cctacaatag atgatattat aaagtctcat ctagtatctg aatataatag agatgaaact
    10141 gtattagaat catatagaac aaattctttg agaaaaataa atagtaatca tgggatagat
    10201 atcagggcta atagtttgtt tacagaacaa gagttattaa atatttatag tcaggagttg
    10261 ttaaatcgtg gaaatttagc tgcagcatct gacatagtaa gattattagc cctaaaaaat
    10321 tttggcggag tatatttaga tgttgatatg cttccaggta ttcactctga tttatttaaa
    10381 acaatatcta gacctagctc tattggacta gaccgttggg aaatgataaa attagaggct
    10441 attatgaagt ataaaaaata tataaataat tatacatcag aaaactttga taaacttgat
    10501 caacaattaa aagataattt taaactcatt atagaaagta aaagtgaaaa atctgagata
    10561 ttttctaaat tagaaaattt aaatgtatct gatcttgaaa ttaaaatagc tttcgcttta
    10621 ggcagtgtta taaatcaagc cttgatatca aaacaaggtt catatcttac taacctagta
    10681 atagaacaag taaaaaatag atatcaattt ttaaaccaac accttaaccc agccatagag
    10741 tctgataata acttcacaga tactactaaa atttttcatg attcattatt taattcagct
    10801 accgcagaaa actctatgtt tttaacaaaa atagcaccat acttacaagt aggttttatg
    10861 ccagaagctc gctccacaat aagtttaagt ggtccaggag cttatgcgtc agcttactat
    10921 gatttcataa atttacaaga aaatactata gaaaaaactt taaaagcatc agatttaata
    10981 gaatttaaat tcccagaaaa taatctatct caattgacag aacaagaaat aaatagtcta
    11041 tggagctttg atcaagcaag tgcaaaatat caatttgaga aatatgtaag agattatact
    11101 ggtggatctc tttctgaaga caatggggta gactttaata aaaatactgc cctcgacaaa
    11161 aactatttat taaataataa aattccatca aacaatgtag aagaagctgg aagtaaaaat
    11221 tatgttcatt atatcataca gttacaagga gatgatataa gttatgaagc aacatgcaat
    11281 ttattttcta aaaatcctaa aaatagtatt attatacaac gaaatatgaa tgaaagtgca
    11341 aaaagctact ttttaagtga tgatggagaa tctattttag aattaaataa atataggata
    11401 cctgaaagat taaaaaataa ggaaaaagta aaagtaacct ttattggaca tggtaaagat
    11461 gaattcaaca caagcgaatt tgctagatta agtgtagatt cactttccaa tgagataagt
    11521 tcatttttag ataccataaa attagatata tcacctaaaa atgtagaagt aaacttactt
    11581 ggatgtaata tgtttagtta tgattttaat gttgaagaaa cttatcctgg gaagttgcta
    11641 ttaagtatta tggacaaaat tacttccact ttacctgatg taaataaaaa ttctattact
    11701 ataggagcaa atcaatatga agtaagaatt aatagtgagg gaagaaaaga acttctggct
    11761 cactcaggta aatggataaa taaagaagaa gctattatga gcgatttatc tagtaaagaa
    11821 tacatttttt ttgattctat agataataag ctaaaagcaa agtccaagaa tattccagga
    11881 ttagcatcaa tatcagaaga tataaaaaca ttattacttg atgcaagtgt tagtcctgat
    11941 acaaaattta ttttaaataa tcttaagctt aatattgaat cttctattgg tgattacatt
    12001 tattatgaaa aattagagcc tgttaaaaat ataattcaca attctataga tgatttaata
    12061 gatgagttca atctacttga aaatgtatct gatgaattat atgaattaaa aaaattaaat
    12121 aatctagatg agaagtattt aatatctttt gaagatatct caaaaaataa ttcaacttac
    12181 tctgtaagat ttattaacaa aagtaatggt gagtcagttt atgtagaaac agaaaaagaa
    12241 attttttcaa aatatagcga acatattaca aaagaaataa gtactataaa gaatagtata
    12301 attacagatg ttaatggtaa tttattggat aatatacagt tagatcatac ttctcaagtt
    12361 aatacattaa acgcagcatt ctttattcaa tcattaatag attatagtag caataaagat
    12421 gtactgaatg atttaagtac ctcagttaag gttcaacttt atgctcaact atttagtaca
    12481 ggtttaaata ctatatatga ctctatccaa ttagtaaatt taatatcaaa tgcagtaaat
    12541 gatactataa atgtactacc tacaataaca gaggggatac ctattgtatc tactatatta
    12601 gacggaataa acttaggtgc agcaattaag gaattactag acgaacatga cccattacta
    12661 aaaaaagaat tagaagctaa ggtgggtgtt ttagcaataa atatgtcatt atctatagct
    12721 gcaactgtag cttcaattgt tggaataggt gctgaagtta ctattttctt attacctata
    12781 gctggtatat ctgcaggaat accttcatta gttaataatg aattaatatt gcatgataag
    12841 gcaacttcag tggtaaacta ttttaatcat ttgtctgaat ctaaaaaata tggccctctt
    12901 aagacagaag atgataaaat tttagttcct attgatgatt tagtaatatc agaaatagat
    12961 tttaataata attcgataaa actaggaaca tgtaatatat tagcaatgga ggggggatca
    13021 ggacacacag tgactggtaa tatagatcac tttttctcat ctccatctat aagttctcat
    13081 attccttcat tatcaattta ttctgcaata ggtatagaaa cagaaaatct agatttttca
    13141 aaaaaaataa tgatgttacc taatgctcct tcaagagtgt tttggtggga aactggagca
    13201 gttccaggtt taagatcatt ggaaaatgac ggaactagat tacttgattc aataagagat
    13261 ttatacccag gtaaatttta ctggagattc tatgcttttt tcgattatgc aataactaca
    13321 ttaaaaccag tttatgaaga cactaatatt aaaattaaac tagataaaga tactagaaac
    13381 ttcataatgc caactataac tactaacgaa attagaaaca aattatctta ttcatttgat
    13441 ggagcaggag gaacttactc tttattatta tcttcatatc caatatcaac gaatataaat
    13501 ttatctaaag atgatttatg gatatttaat attgataatg aagtaagaga aatatctata
    13561 gaaaatggta ctattaaaaa aggaaagtta ataaaagatg ttttaagtaa aattgatata
    13621 aataaaaata aacttattat aggcaatcaa acaatagatt tttcaggcga tatagataat
    13681 aaagatagat atatattctt gacttgtgag ttagatgata aaattagttt aataatagaa
    13741 ataaatcttg ttgcaaaatc ttatagtttg ttattgtctg gggataaaaa ttatttgata
    13801 tccaatttat ctaatactat tgagaaaatc aatactttag gcctagatag taaaaatata
    13861 gcgtacaatt acactgatga atctaataat aaatattttg gagctatatc taaaacaagt
    13921 caaaaaagca taatacatta taaaaaagac agtaaaaata tattagaatt ttataatgac
    13981 agtacattag aatttaacag taaagatttt attgctgaag atataaatgt atttatgaaa
    14041 gatgatatta atactataac aggaaaatac tatgttgata ataatactga taaaagtata
    14101 gatttctcta tttctttagt tagtaaaaat caagtaaaag taaatggatt atatttaaat
    14161 gaatccgtat actcatctta ccttgatttt gtgaaaaatt cagatggaca ccataatact
    14221 tctaatttta tgaatttatt tttggacaat ataagtttct ggaaattgtt tgggtttgaa
    14281 aatataaatt ttgtaatcga taaatacttt acccttgttg gtaaaactaa tcttggatat
    14341 gtagaattta tttgtgacaa taataaaaat atagatatat attttggtga atggaaaaca
    14401 tcgtcatcta aaagcactat atttagcgga aatggtagaa atgttgtagt agagcctata
    14461 tataatcctg atacgggtga agatatatct acttcactag atttttccta tgaacctctc
    14521 tatggaatag atagatatat caataaagta ttgatagcac ctgatttata tacaagttta
    14581 ataaatatta ataccaatta ttattcaaat gagtactacc ctgagattat agttcttaac
    14641 ccaaatacat tccacaaaaa agtaaatata aatttagata gttcttcttt tgagtataaa
    14701 tggtctacag aaggaagtga ctttatttta gttagatact tagaagaaag taataaaaaa
    14761 atattacaaa aaataagaat caaaggtatc ttatctaata ctcaatcatt taataaaatg
    14821 agtatagatt ttaaagatat taaaaaacta tcattaggat atataatgag taattttaaa
    14881 tcatttaatt ctgaaaatga attagataga gatcatttag gatttaaaat aatagataat
    14941 aaaacttatt actatgatga agatagtaaa ttagttaaag gattaatcaa tataaataat
    15001 tcattattct attttgatcc tatagaattt aacttagtaa ctggatggca aactatcaat
    15061 ggtaaaaaat attattttga tataaatact ggagcagctt taactagtta taaaattatt
    15121 aatggtaaac acttttattt taataatgat ggtgtgatgc agttgggagt atttaaagga
    15181 cctgatggat ttgaatattt tgcacctgcc aatactcaaa ataataacat agaaggtcag
    15241 gctatagttt atcaaagtaa attcttaact ttgaatggca aaaaatatta ttttgataat
    15301 gactcaaaag cagtcactgg atggagaatt attaacaatg agaaatatta ctttaatcct
    15361 aataatgcta ttgctgcagt cggattgcaa gtaattgaca ataataagta ttatttcaat
    15421 cctgacactg ctatcatctc aaaaggttgg cagactgtta atggtagtag atactacttt
    15481 gatactgata ccgctattgc ctttaatggt tataaaacta ttgatggtaa acacttttat
    15541 tttgatagtg attgtgtagt gaaaataggt gtgtttagta cctctaatgg atttgaatat
    15601 tttgcacctg ctaatactta taataataac atagaaggtc aggctatagt ttatcaaagt
    15661 aaattcttaa ctttgaatgg taaaaaatat tactttgata ataactcaaa agcagttacc
    15721 ggatggcaaa ctattgatag taaaaaatat tactttaata ctaacactgc tgaagcagct
    15781 actggatggc aaactattga tggtaaaaaa tattacttta atactaacac tgctgaagca
    15841 gctactggat ggcaaactat tgatggtaaa aaatattact ttaatactaa cactgctata
    15901 gcttcaactg gttatacaat tattaatggt aaacattttt attttaatac tgatggtatt
    15961 atgcagatag gagtgtttaa aggacctaat ggatttgaat attttgcacc tgctaatacg
    16021 gatgctaaca acatagaagg tcaagctata ctttaccaaa atgaattctt aactttgaat
    16081 ggtaaaaaat attactttgg tagtgactca aaagcagtta ctggatggag aattattaac
    16141 aataagaaat attactttaa tcctaataat gctattgctg caattcatct atgcactata
    16201 aataatgaca agtattactt tagttatgat ggaattcttc aaaatggata tattactatt
    16261 gaaagaaata atttctattt tgatgctaat aatgaatcta aaatggtaac aggagtattt
    16321 aaaggaccta atggatttga gtattttgca cctgctaata ctcacaataa taacatagaa
    16381 ggtcaggcta tagtttacca gaacaaattc ttaactttga atggcaaaaa atattatttt
    16441 gataatgact caaaagcagt tactggatgg caaaccattg atggtaaaaa atattacttt
    16501 aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac
    16561 tttaatctta acactgctga agcagctact ggatggcaaa ctattgatgg taaaaaatat
    16621 tactttaata ctaacacttt catagcctca actggttata caagtattaa tggtaaacat
    16681 ttttatttta atactgatgg tattatgcag ataggagtgt ttaaaggacc taatggattt
    16741 gaatactttg cacctgctaa tactcataat aataacatag aaggtcaagc tatactttac
    16801 caaaataaat tcttaacttt gaatggtaaa aaatattact ttggtagtga ctcaaaagca
    16861 gttaccggat tgcgaactat tgatggtaaa aaatattact ttaatactaa cactgctgtt
    16921 gcagttactg gatggcaaac tattaatggt aaaaaatact actttaatac taacacttct
    16981 atagcttcaa ctggttatac aattattagt ggtaaacatt tttattttaa tactgatggt
    17041 attatgcaga taggagtgtt taaaggacct gatggatttg aatactttgc acctgctaat
    17101 acagatgcta acaatataga aggtcaagct atacgttatc aaaatagatt cctatattta
    17161 catgacaata tatattattt tggtaataat tcaaaagcgg ctactggttg ggtaactatt
    17221 gatggtaata gatattactt cgagcctaat acagctatgg gtgcgaatgg ttataaaact
    17281 attgataata aaaattttta ctttagaaat ggtttacctc agataggagt gtttaaaggg
    17341 tctaatggat ttgaatactt tgcacctgct aatacggatg ctaacaatat agaaggtcaa
    17401 gctatacgtt atcaaaatag attcctacat ttacttggaa aaatatatta ctttggtaat
    17461 aattcaaaag cagttactgg atggcaaact attaatggta aagtatatta ctttatgcct
    17521 gatactgcta tggctgcagc tggtggactt ttcgagattg atggtgttat atatttcttt
    17581 ggtgttgatg gagtaaaagc ccctgggata tatggctaaa atatatgttt gataaaaaat
    17641 tattcctgtg ctactaagaa attattttta tataataaat attgagattt aattaaaagt
    17701 catgtgttat tgtaatacat gacttttagt taaaattttt ctatcattta ataatctatt
    17761 attcttgact attttataat aaaattcata tatggaaata ttaatactaa ataattaata
    17821 gttgataaaa aatagataat atgctaaaag caaaaactaa tttagagcct tgtaactgtt
    17881 tatttgcaat tataaaaaca tctttaaaca tattgactat aatataaaat attaactata
    17941 atacaaaaca atattaatta attttctcta cagctatccc tggtatagtt atatttccac
    18001 ccatagttga ttcataagtt atagttccag cacttatacc tcttatagtt atgtaatcat
    18061 cctctaatac tcttgaagga gttattgatt ttttataact acatagtaca accttatcat
    18121 aatttccaga cacagctaat cttatttgca cctcatcacc atcttcaata acttgaataa
    18181 ccttaccttc aaattttacc tttttgtact tataatcatc aggtgttcta gctaattggt
    18241 cataagtaat accagtatca tatcctttct tctcctcttc ttcttttttg cgttgttctt
    18301 cagccttttt agcttcttca gcttttttag cttcttcagc tttacgttga ttttcagctt
    18361 caatagcttt cttttcgtcg tctttcattt tgaaccatgg ttcagcatca gacaatttgc
    18421 tatttaaagt ttctatttct gttttttgag aatctataac tgatgtttgg ttagaaatga
    18481 cctcctcatg gtcttcagaa caagatggtg aggatatatt gccaaaaaac acgcctaaaa
    18541 taaatgccag tagagcaata ccctttgtgc tcttaaagaa tttaattatt ttcttagagc
    18601 ttccttttcc ttcattacta aattcgttac cctcattttt tttagaaaac ataatacaat
    18661 ctccctttta ttagatatat tttattttaa aagttaaaaa cttaaaataa ctagttgact
    18721 aaaaattata tattcatatt ttcctaaaat atatttcccc taagcataat tt
//
LOCUS       DAMJYC010000080        18641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJYC010000080 DAMJYC010000000
VERSION     DAMJYC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.081"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            148..1365
                     /locus_tag="PC472_002898"
     CDS             148..1365
                     /locus_tag="PC472_002898"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2728207.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1460..1840
                     /locus_tag="PC472_002899"
     CDS             1460..1840
                     /locus_tag="PC472_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2728208.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2124..2606
                     /locus_tag="PC472_002900"
     CDS             2124..2606
                     /locus_tag="PC472_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2728209.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2627..3442
                     /locus_tag="PC472_002901"
     CDS             2627..3442
                     /locus_tag="PC472_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2728210.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3495..5045
                     /locus_tag="PC472_002902"
     CDS             3495..5045
                     /locus_tag="PC472_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2728211.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5142..6494
                     /locus_tag="PC472_002903"
     CDS             5142..6494
                     /locus_tag="PC472_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2728212.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGLHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6625..7383
                     /locus_tag="PC472_002904"
     CDS             6625..7383
                     /locus_tag="PC472_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728213.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7430..8149
                     /locus_tag="PC472_002905"
     CDS             7430..8149
                     /locus_tag="PC472_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2728214.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8273..9673)
                     /locus_tag="PC472_002906"
     CDS             complement(8273..9673)
                     /locus_tag="PC472_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2728215.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9677..10585)
                     /locus_tag="PC472_002907"
     CDS             complement(9677..10585)
                     /locus_tag="PC472_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728216.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10846..11493
                     /locus_tag="PC472_002908"
     CDS             10846..11493
                     /locus_tag="PC472_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2728217.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11818..14457
                     /locus_tag="PC472_002909"
     CDS             11818..14457
                     /locus_tag="PC472_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2728218.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14515..15618)
                     /locus_tag="PC472_002910"
     CDS             complement(14515..15618)
                     /locus_tag="PC472_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2728219.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15979..16800
                     /locus_tag="PC472_002911"
     CDS             15979..16800
                     /locus_tag="PC472_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2728220.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16879..17090
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17177..18493
                     /gene="argH"
                     /locus_tag="PC472_002912"
     CDS             17177..18493
                     /gene="argH"
                     /locus_tag="PC472_002912"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2728221.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7123 a         2149 c         3303 g         6066 t
ORIGIN      
        1 tattgtatgc aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat
       61 cgtttacatg ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa
      121 aaattggatt tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtaaaagag
      181 gctagagaga ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt
      241 agcgaaaaaa caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct
      301 tttaaattaa gaggagcttg taacaaaata gcgagtttaa cagatgaaga aaaggcaaat
      361 ggagttatag cttcaagtgc agggaatcat gctcaaggag ttgcattggg agcaaaaatg
      421 actggtataa aatctacaat agtaatgcca gcaactgcgc ctttagcaaa agttagtgca
      481 actaaaggtt atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca
      541 aaagctgttg aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag
      601 tatgttatag caggacaagg aactatatct cttgaaattt tcgaacaatt agacaataaa
      661 gttgatacta tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca
      721 gcaaaagcat taaatcctaa tgtaaaaata gtcggtgttc aaactgcaaa tataccttca
      781 atgaaagagt caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct
      841 gatggtatag ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattggtt
      901 gatgaaatag tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa
      961 agtcaaaaaa tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt
     1021 aagtatgtac cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac
     1081 ataaatacgt tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca
     1141 tttagtacaa ttatggaaga taagccagga aactttgctg agttaactag aataataagt
     1201 gagaatggtg gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta
     1261 ggaaaacaaa gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata
     1321 aagaaagcta tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata
     1381 atatacaaga aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac
     1441 gaattaggag gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc
     1501 tttaggacca tattctcaag ctataaaagc tggaaacttg ttatttgtat ctggacaagt
     1561 tcctcttgta ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca
     1621 atctttaaaa aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt
     1681 taagactaca gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc
     1741 tgaatatttt ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa
     1801 agacgttaaa gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt
     1861 gaaaaggctg aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa
     1921 taaagaacat gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat
     1981 ttttgagaag gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat
     2041 gagttcttgg atatttttat cctgaggatt catgcctttt ttgtattgaa attcaaaaat
     2101 ctatttaata aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt
     2161 tacatgcagt agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt
     2221 caaaaaagtt aatgggagat ggagttgcaa ttattcctaa tagtgatgtt gttgtagccc
     2281 cttgtaatgg taaagtaact gtgcttacag aatcaaagca tgcctttggt atggtctcag
     2341 atgagggtgt tgaaatatta gtccacatag gaattgatac agttagttta cagggtgagg
     2401 ggtttaaaaa tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagtt
     2461 ttgaaagaga aaaaataaat tctcagggaa ttgattgtac aacaataata attgtattaa
     2521 atcatagcga attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagggcaag
     2581 atactgttat tgaaattatg aaataatagg aagggattaa ttcaatatga gagtagtaaa
     2641 aatctttaat aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact
     2701 aggtcttggt ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga
     2761 aaagatttat tatgttcaag accacatgca aacaaaattt ttagaattat taaaaaatgt
     2821 tagtccagaa gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg
     2881 aaaatttaat aataaaggga tattatcttt agtggaacat attagttttg caatagatag
     2941 aatgaaaaat aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc
     3001 aaaagagttt gaattaggag taaaagcttt agaaattata tatagagtat gtcatatcca
     3061 tttgcctgaa gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga
     3121 taatttggca tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg
     3181 ttatggatta gaattagacg aatcgagttt aagtacatta cgttttagaa ctcatcttaa
     3241 atttcttgct caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat
     3301 gtatgattat ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt
     3361 atatattgaa aaggagttta ggtataaatt agataaacct gaaaaaattt atctactact
     3421 acacctaaca aaaatcttat aagaatgaga tgaattagta atagaaatat atataatttt
     3481 agggggggat aagattgaaa gaaaaactac ttggaggact ggaaacattt gcaaaggcaa
     3541 tggttcagcc attaatgtat ttatcgagtg caggaatatt aatggtggta ggtgtactac
     3601 ttacaaatga tatgttatta ggagtacttc cattttttaa atgggagcca attcaaatat
     3661 ttggaagcct aatttataac tgtataatgg ctattattaa taatcttgct gttatatttg
     3721 ctataggtat tcctgctgca ctagcaaaaa aagagaagca caaagcagcg ttaataggat
     3781 ttatgagtta tataatatac cttacagctt ccaacactat gttaacatca ctaggtcaac
     3841 tagcaaaaga aagcaagatg cttggactga tagggactgg acaagctact attttaggta
     3901 ttcaagttgt agatacaagt gtatttggtg gaattatttt agggtgtata gctggatatg
     3961 tatttaatcg tacaagcaac aaaacattta aaggagcttt acagatatac tcgggagcaa
     4021 attttagttt tatatgcatg ttctttgtat ccattttaat tggagttgtg agtaacttta
     4081 tatggccatt agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa gatacaggta
     4141 catttggttt attcttatat ggttttttag agagaatatt agtaccaact ggacttcatc
     4201 atttaattta tacaccattt cagttttctg atttaggagg agtgcttaaa gttggtgcag
     4261 atacagtttc aggagcctat gctatagtta tgacagaaat gaatttacca gttgagcgtt
     4321 tctcagatag tatatactat atggcaactg gatttacaaa gatgtttggt tatataggta
     4381 taggagctgc ctttatttat actgcatata agcaaaataa gaagaaggtt aaggcaacga
     4441 ttattccact aataattaca gcagtattag cctctgtaac agaaccatta gactttatgt
     4501 ttgtattctg tgcaccactt ttattctttg tacatgcagt tatagctggt ctttttatag
     4561 ctttattaaa aatatttgat gttactgcat tatgtggagg gaacttatta gtatcaatta
     4621 taatgaatac tacagtaggc gtagaaaaaa cacactggcc agtaatgatg atattaggac
     4681 ttgcacaaat tattgtatat tttgtagtat ttagtttttt aattaagaaa tttaattata
     4741 aaactccagg tcgtgaagat gagagtacag ctacaggcga agaacaatca aaacaactta
     4801 atttagatgc agggatagaa aatataattg atgggttagg cggaaaagaa aatattaata
     4861 cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa agatgaaagt aagattaatg
     4921 aagatataat taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa gatatacaaa
     4981 taatttttgg acttcatgtg catgaggtaa gacgtgcagt agaagatttt ttagaagctt
     5041 attaaagata tatatttata tagcaagggt aaataacagc tttaagtgct gtttactatt
     5101 tattaaagat acatataatt tatagaggag gaataattat tatgattatt actattgttg
     5161 gtggaggaag cacatttact ccaggtattg ttaagtcaat tgcattaaga gaaaaagagt
     5221 tagagcttga ggaaattcgt ttatttgata ttgataaaaa acgtcaagat aaagtagctg
     5281 tagttgtcaa ctggatacta aaagaagatg ttaaaacaga tgttaaacta actgtaacta
     5341 attgtgaaaa agaagcgtat acagatgcta catttatatt tgcacaaatg cgtgttggta
     5401 aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt gtaggtcaag
     5461 aaacttgtgg ttgtggaggg atggcatacg gactgcgtac aatacaacca atgataaagg
     5521 ttattgatga tgtagagaaa tatgctaaaa aagattattg gatattaaat tactctaatc
     5581 ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg attataaata
     5641 tctgtgatat gccaatagct attatagata tgatagcggg gtcattaaat atcaatgacg
     5701 ttcataatat acgctatgat tattttggtc taaatcattt tggctggttt acttcaatag
     5761 attataaaca tagagactta atggaagaga ttaaggaata cataaaagaa aataaaatat
     5821 tattaccaaa atcttatata gaaaacttaa ataaaagtcg tagtacagga aatcgccatg
     5881 ctgtaagtag ctggatacat gtttgggaat ctgtttatac aatgttagaa agcttcccaa
     5941 aatatctgcc aaacacttat ttaacatact atcttttaag taaagaaaca gtggaacatt
     6001 caaacataaa tcgtactcgt gcaaatgagg ttatggaaag ccgtgaaaaa gacttatttg
     6061 aaggtgtgag acattactta gaaactggag aaatttctga aaaagcattt tatgcaggtt
     6121 tacatggaga ttggattagt gatttggcag tttcaattaa aaatgataca cgtagtcgtt
     6181 ttttagttat aactgaaaat cgtggagcta taccaaacat gccatatgat gcaatggtag
     6241 aaattccagc atatattggt aaagatggtc cagaagttat tgctcgaggt ccaatacctc
     6301 tatttcaaca aggattaatg atgcaacaac ttaacagtga aaaattgata gtagaagctt
     6361 atatagaaaa tagttatgaa aaagctttac aagcattcac attaaataaa actattccaa
     6421 gtatgaatgt tgctaaagaa attttggatg aaatgataga agttaataag gaatactggc
     6481 cagaattaag atagtaggat aaaagaaagg gtttttattt aatcattaga tatgttttca
     6541 gagtctttta tcaaaatgta ttcacaaaca tatggcaata tattaaaata aagagtgata
     6601 tttttagaag aatggggtga tgcaatgaac attataaata aaattttttg gcaagaagat
     6661 aggattggca tgattacaaa taatttagag gcagatacac atagtcactg tatgttacaa
     6721 ctgttcttag ggatagaaga cagcattgaa ataacagtta atgaaaaatt agttaaatgt
     6781 aattgtatca tagtagataa aaatatctct cattcatttt cagcaagaaa aaaagtttat
     6841 tactctgcta ttattgaacc tacatctata tatgcagaac agcttacctc aaaaatgaat
     6901 gattttggct actggatatg tgacaataat ggattagaga aattacgtca acaaggaact
     6961 tttttgatag ataactctag taaagaacaa tatttaagat ttatggaaat gcttaataac
     7021 tatttgaata tacctataac tctaaaacat tatgatgaca gaattactga attgctcaat
     7081 ttattacgta cttgtaattg tgataatcat actatttcaa gttttgcaga taaagtgtct
     7141 ctatcagcaa gtagattatc acatctgttt aaagaacaaa tagggatacc tttaaaaagc
     7201 tatatcttat ttcatcaaat ggagtgtgct tttagagaat tactttctgg aaaaaatgtt
     7261 accgaagctt ctatgactgc tggattcgat actccatcac actttgcagg gacagttaag
     7321 cgaatgatgg gaatgcctgt ttctttatct ttgaaagata gcgagttttt gaaagtatat
     7381 taaatattct cgttatataa tgaagttgta ctaagaaagg ggctttgtta tgaataaaga
     7441 atatttagaa gaaacaaaaa tgttaaatta taatgaacca caacttaagc ttttggtatc
     7501 atcaaagaat tggttggaat tagatgattt tcataaaata aaatctattt atgagtttgt
     7561 acagaatgat attttatttg gatataatgc ttctgatatg ttaagcgcaa cacaggtatt
     7621 aaatgatggt atgggacaat gcaatacaaa agctactctt ttaatggcat tattacgtgc
     7681 agtgaatatc ccctgtaggc ttcatgcttt tgatgtaaca aaagattttc aacgaggagc
     7741 tacatccaaa ttgatttcac tattagcacc aaaatatatt cttcatactt gggtagaggt
     7801 attttatcaa gataggtgga ttgctctgga gggtgtcata actgataaaa aatatttaga
     7861 agcaattcag aagaaatttt ttaatcatgg tggaacgttt aaaaagtatg caatagctac
     7921 aaatgatttg aaaaatacat ctattgattg ggatggtaaa gatactttta tccaaaaaga
     7981 agctatcgtg tatgattatg gtatttttcc atcaccagac gtattttttt ctactcattc
     8041 acaacatatg tcaaaattaa aaaatttcat atatgtacat cttatcagaa aaataatgac
     8101 aaaaaatgta tgtaaggcta gaaacaatta tattgataaa aatgaatgag tgtttaagag
     8161 gtaactatat aggaaatata tagaataatt aaaactgtac tggttaaaat gaataaaaag
     8221 gagttatcac agagcaaact taaattgttc ataggataac tccttttctg tattaacttc
     8281 taataatttg atgtcttttt ataaaacata tagatacaat taaaccagta aatgcaagag
     8341 tagctgctat tgaaaatgca acatctattc catgaatgtt agacagcatg tttgaactca
     8401 tatgagcttt cttagtagca cttgtcatta tagtaataag tatagcagaa cctatagctc
     8461 cagatatctg acggagtgtg ttattaattg ctgtggcatg agatataagt tctctatcta
     8521 atgttttaat tccccatgta gttaatggca ttaaaaccat tgaaatacca atcatacgaa
     8581 tacaatacat caatgacaca tgaattaaag atgtatctct gcctaaaaaa gcgaaggata
     8641 aagttcctaa aaacaaacat cctgttccta atattgatag agcatgaggt ccatatttat
     8701 ctaaatgacg tcctgcaatt ggattgagta caaccatcaa tatagcacca ggtaacatta
     8761 atgaacctga agataatgca gaaaatcctc ttacagattg aacgtacaga ggaatcatta
     8821 atgtagcaga catcattgag gcatatacaa tcataattaa tattgtagaa ataagaaatg
     8881 gtttattttt aaaaaccctc aattctaaaa aaggttcttt agattttaat tgacgcaatg
     8941 taaataaaat taatgacata attcctatta atattggtaa atatgtggca atattagtcc
     9001 acccataatt accttgatta gtaaatccaa ttaataaacc accaaaacct agagatgata
     9061 gtactattga aggtatatct aattttagtt tttgagtttc cccaacattt cttaataaaa
     9121 taaatgatag tataacatct aatatagcaa taggggaaag aaacagaaat aaactatgcc
     9181 aaccaaatga atctactaac catcctgata aagttggacc tattgctgga gcaaatccta
     9241 ctgtaatacc aactaaactc atagctgcac cacgtttttc ctcagggcat aaatagagta
     9301 taataacttg taaaagtggc attagagaac cagaacccat tgcctgcaac acacgagcaa
     9361 tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat acatcctata gaaaagatag
     9421 acattgatgt aataaataac tttctagtac taaatcgatt tattaaataa gctgttgtgg
     9481 gtacaataat acctgcaata agtagataaa ttgttgttaa ccactgacca acatcagcag
     9541 taattttaaa atctgacatt atattaggta atgctgttga caatagtgtt tgactgagag
     9601 aacttataaa agcaccagtc attacaattg ctattattaa atttcctttt ttattgttac
     9661 tttgggtttc cataatttat aacgtctcct tttttaattg atttttaatg ctgttcaata
     9721 aaagtacttt tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt gcatccataa
     9781 ttaaagaatc atattgttga ttttttccct cgacgtataa tttttttatt gcttctagta
     9841 atttaggatt ttcaaggaga gaagtatccg ttttgattag ttcttctata tcttgtatcg
     9901 ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac ttcatcttct tgtttatgta
     9961 aaaacattga tttttcaatt aaaggattta gattatccat attttctaca actaaatcta
    10021 gaatttgtat agtaaatttg gcaataaagt atacatttat ttctcttttt tgtacatgaa
    10081 taatgtatat aaactgatga attagagcat ccaataagaa aactaaatca aatataattt
    10141 tttctatttt atcaccatag ttatcaaata agcttagttt gtattcattc aataagttag
    10201 ctcttgattt tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa aagttttctc
    10261 ttatataggt atttatggta gttctagaaa aaatattttt ccatacagca attatctttc
    10321 tgtttaactt ttctgatgat ggaatgtcat tattttcatt tatggaatgt ataatttcta
    10381 aaaacttttc atcataataa ttgattattt cgcagataaa actttcctta gtttcaaaat
    10441 gcttgtaaaa agtagcttta gacattttac actcttttac aacatccgat attgaagtat
    10501 tttgaaaacc tttttggtca aaaatttttt gtgcaatagt tataatgagc ctttgttttt
    10561 ctgtcacttt attatctcct ttcacgcaaa aacgtttagt actatatagt acctacaagt
    10621 tttgataaat atattataca tcatttggaa aaaaaatcaa tatatagact gtaatatgaa
    10681 atttttgaaa aatttgttgt tatttttagt cgtataaaaa agaaacttaa gagtttctta
    10741 agaattgttt aaagaagttg caataaatta gtggtatgat tatagtatga aagagaatgt
    10801 cttgaaattt ttctgtatca aattccaatt atggaggtgt agtatatgaa aaagaaaatt
    10861 tgtgtagcaa taatttttgc tctatttata gtttttgtag aaatgtttat tgatagctct
    10921 agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat acaggaaagg cgtaaatgta
    10981 ttatcaataa acaatattat agtagcgaat aaaaaatata gtttgcccaa agattattct
    11041 cctcaggaaa gcagtgaagc gagagatgct ttttataaaa tgaataaaga tgctcaaaag
    11101 tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta
    11161 tttaagtcat atgtaaaaga acatggagaa aaagaagcaa atagattttc agctaaacct
    11221 ggagagagtg agcatcaaac tggacttgct tttgatattg gaggagatga ccagtcttgt
    11281 tgggcaaata aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat
    11341 aaatatggtt ttatacttag atatccagaa ggtaaagaga atataacagg atatatgtat
    11401 gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc aatgaataat
    11461 ttgactttag aagaatattt acatataaat taagagatgg tttgttaaaa ttaggttaaa
    11521 ataaaaaata acctagacta ttagctgtgt aaagtgatat aatgtaaata caatttaaga
    11581 taactactcg aaggggagta gcttacacga aagtggtaat taatcgtcaa gacagtagaa
    11641 atactcggtt aattagcaag ggattccttg atagcaagac cttcaaaata agcattaggt
    11701 atgtttgttt tggggtcttt ttttattcaa ggaattttta tttaaaaaaa tttaaatttc
    11761 aagtatacat aagagccccc aaacataaaa taaatttatt gaagaggagg gcaacaaatg
    11821 tcttacaaaa gtacttatca agaagtaatt aaaaatttag actcaagttt atctggtttg
    11881 agtaaagaaa gagcagaaga gttgttagaa aaaaatggag ctaatgaatt aaaagaagca
    11941 gataaggttc ctacttataa gcttttccta gaaagtttta aagacccatt agtaataatt
    12001 ctattgatag ctgctttagt acaaatattt ttaggtgaaa cagtggagtc tataataatt
    12061 tttgcagtta ttattataaa ctctgttctg ggtgttgtac aaacaaaaaa agcagaaagt
    12121 tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag agacaataaa
    12181 aaaatgacaa ttccagcaaa aaatttagta gttggagata ttgtattctt agaagctggc
    12241 gattacatac cagcagatgg aaggcttata gaagcacaat ctctcaaggt tgtagaaggg
    12301 atgttaactg gagaatcaga accagtttta aagcatacag aaaaaataag tgaagatgta
    12361 agtttaggtg accagaaaaa tatggtattt agtggtgctg tagttgttta tggtagaggt
    12421 tcttttgtag tgactggaac aggtatggat actgagatgg gtaaaatagc tgggctttta
    12481 gaaagtgctg aaaataaact tacaccattg caacaaaaaa tagaggattt tagtaaaaaa
    12541 cttggtgtag gtattgtaat acttgctctt ttaatattta taattcaagt agcaagaaat
    12601 tactttatgg ctggaaatgg agaaatgaca caaattattt tagattcatt catgttctct
    12661 gtagcagtag cagtagcagc tataccagaa gctctatcat caatagtcac tatagtattg
    12721 gctgttggaa caaatagtat ggcaaaaaaa caagccataa taagaaaatt accagcagta
    12781 gaaactttag gttcaacaag tgtaatatgt actgataaaa caggtactct tacacaaaat
    12841 aaaatgacag tagttgatta ttatatgtat ggaactaata atgatgagtt agataaagaa
    12901 aaagtaaact attcttatca tgctaaatta ttgactatgg tttctacatt gtgtaatgac
    12961 tctcatataa ctagtgatgg taaagaagtt ggtgacccaa cagaagtggc tttaataaat
    13021 tattcaagta aaaatgattt agattatgat aaattaagaa gtaagtatat cagaataaat
    13081 gaaataccat ttgactcaga cagaaagctt atgtctactg taaatagtat atatggagat
    13141 tatataatgt ttacaaaagg tgctccagat attgtgttta gtagatgtaa atatgctttg
    13201 aaaaatggtt caaaagtaga tataacagat gagataatag aagaatataa acataaaaat
    13261 gaagagttct caaatagagc acttagagta ttagcttttg caataaaaga tgtcccagaa
    13321 gaagatttta taccttcaat tgatgacgaa catgacatga cattggttgg aataatggca
    13381 atgatagacc caccaagaga agaagtaatg gatgcagtaa aagaggcaaa gagtgctggt
    13441 ataaaaactg ttatgataac tggtgaccat aaaacaacag cagcagcaat tgcaaaagag
    13501 attggtataa tggaagaagg agacttggct cttactggaa aagagcttga tgctttgagt
    13561 gaagaacaac tatatgaaaa actagaaaat ataagtgtat atgccagagt ctcgccagaa
    13621 aataaaattc gtatagtaaa agcatggcaa cataagaata atataacagc aatgactggt
    13681 gatggtgtta atgatgcacc tgcattaaaa caagcagata taggaatcgg tatgggaagt
    13741 ggtactgatg tagcaaaaga tgcttctgct atggtattgg ttgatgataa ctttgcaagt
    13801 atagtaaatg ctgttgaagt tggtagaaca gtatacagta atataataaa atcaattact
    13861 tatctatttg caggaaactt aggagctata gtatccatag tatttgcagt gtttgcaggt
    13921 tggtcaaatc catttacagc tttacaactt ttgtttataa acttagtaaa tgactcttta
    13981 ccagcaattg cattagggtt tgaaaagtca gaaaaaaata taatgaataa acctcctaga
    14041 cacccggatg aaagtatact agcaggagga acattgaagg cagttgctat aagaggttgt
    14101 ataataggtg tagttacaat aattgcacaa tatataggat tacaagtatc tccagaactt
    14161 ggagtagcaa tggcattttc aacattgata ttagctagaa taatgcaaac attacctgca
    14221 cgttctaata atcaaacagt aataaaatta ggttttttca gcaataaata cgttatagga
    14281 gcagttttag tctgtcttgg gctttactct ttaacactaa taccagtgct aagaggaata
    14341 ttctctatac caacaacgtt tggattgtac cagttgggga tttgcctagg gttagcatta
    14401 atttcatcaa tattaatgga acttaacaaa ttaataaata gaagtgaagt aaaataatca
    14461 aaaaattaaa gggcatattg attttaacaa tatgcctttt ataaatagtc taatttattc
    14521 tatattatcg attatttgga atgcttttat aacttcaggt aattcttttt cttcaggcat
    14581 tctaaatctt atgaaatttt tatcaagtcc agtaaaatca cttccagata taactcttat
    14641 ttttaattta gcaaactctt gttgtaaatc tatgtccttg tttttatgtt caactgtcat
    14701 aattgataca gtatcactag tttcagctat attaaggttt ttccatggaa ttaataattg
    14761 attttttata tcttttgttt tttcttttaa ttcttctata aattgaacat catctaataa
    14821 atttgctgca atactacgag atatttcaga cacttcgtaa ggagtagaaa tctttttaat
    14881 agggctaaca agttgttctg gaagaactgc atatcctgct cttagtcctg ctaaaccaaa
    14941 tccttttgaa aatgttttta aagcaattac attatcataa ttatttagaa gcttgacagc
    15001 tgaattttct tttggcatgt attcaccata agcttcatct accataactg ctatatcata
    15061 tttagctgct tcctttacta tattttcaat tgatgataaa ggtataattt gtcctgtagg
    15121 attgtttgga ttgtctatgt aaattacttt atattctgga tttatttttt caataaattc
    15181 tttttcatta aacttgaaat tatcctcttt ctttaaaaga acataatcat aagttgcacc
    15241 atgcattttt atatctgtct catattctga gaactgaggt gaatatccta aaactttatc
    15301 tcccttttca ataaatagtc tatttaaaag gtaaataaca tgtatagaac catctgccaa
    15361 acaaattctg tcagtatcta atgctataaa atctttccaa taatcaacta tattatcttt
    15421 taaagcaatt gaatgaggat atcctcttat catatcaaat tttaaagcat taaaagcttc
    15481 tacagctttg tttgagtaag acacggtatt tataccttca ccacagtcta tctcgaatac
    15541 atcttctgaa tgtccagatt ctattgcata actttttttc aactgcattt caatagcttt
    15601 gttaattttt agtttcatac tttatcactc cttatttagt caataataaa tgttataaaa
    15661 gttgctagat ttaaaatatt atgaaaatat ataatagtaa aaaatgaata taaatattat
    15721 aatagctcac tttatttaat atgattatat atcatatatt gtaaaatttc tagcattata
    15781 agactataaa ttgaaaatat tcagaaaaat actacaagaa acgatttatt ttgctcttaa
    15841 aataagttaa atgaatgtta ttaattttgt aaattgaaat agtttaagta taaagttatg
    15901 atataatcta ataagggtaa ttctttagat tatattaagg aaatataggt atacaataaa
    15961 aagttatgga gttggattat gggatataaa ttaattgtaa cagatatgga tggaacttta
    16021 ttaggcaata atcataaagt tacagatgaa aataaaactg cactacaaaa agttataaaa
    16081 agcggtatta atgtaacttt agcaacagga agagccttcg attcagctaa atgcaatgta
    16141 gattttctta aagaggacat gccaataata gcttgtaatg gttcattaat aagagagcaa
    16201 aatggaaata taatatattc aaataagatt gatacaagaa catgtttaaa cattttagat
    16261 gtattagata aatacgatat ttactaccaa tgtaatagta tagattctat gctttcgaaa
    16321 aaaatagaag gacgtgaaga tagattaagt gtatttttag gctctgaaac agaagtaatt
    16381 gtaaaagatg atttgagaga agaaatcttt aaaaaagata tactaaaatt tgtaatcata
    16441 gaagaaaaaa atccatctat tttggatgaa ataagaaaag aattgagaaa agttcaagga
    16501 ataaagataa cttcttcatg gccaaacaat attgaagtca tgaatgaagg tgttgataaa
    16561 ggtaatgcag ttaaaatttt agctgaaaaa atgaatatag acagagaaga tataattgca
    16621 tttggagaca attacaatga catagaaatg ataaagtttg ctggtcttgg ggtagctatg
    16681 ggaaatgcag aagaattgat taaacaagag gctgattatg ttacagatac aaatcaagac
    16741 agtggggttg caaaggcaat ttataaattc ctagagttaa aagaatcact tagctcttaa
    16801 aacagtatat aataggaaaa aataggttga ctattttgta gatagtaatt ataatacaaa
    16861 tagataaaac ataaaaattt agattcagtg aaaaagagag tagatataga gaaaatctca
    16921 gcgaattggg ggatggtgtg agcctaatga taacacttta tattgaaaag aactttggag
    16981 aatactactt gaaattcagt atggtagtag gtaatctcgc cttaagagaa tagagtgggt
    17041 aaacattgtt taccaactag agtggtaaca cggaatattt cgtctcttgg tctttttgac
    17101 caagagattt tttagtttca ttgagttaca agacttataa ttaaaataca aaaaattaaa
    17161 atatgaggtg aaagttatga aattatgggg cggacgcttt agaaaagcgg aaaatcaact
    17221 tatggaagaa tttaataaat cttttggcta tgattgtgta ctgtacaaaa aagatatcga
    17281 aggaagtgta gcacatgttc atatgcaagt aaaatgtgga cttttaacag aagaagaagg
    17341 aaagtctata acagaaggtt taaaagggat acttgaagat gtagaaaatg gaaagttagt
    17401 attggatgga gagtatgaag atatacatag ttttactgag ataaatttaa ttcaaagaat
    17461 tggagatgtt ggcaagaagc ttcatacagc acgtagtaga aatgaccaag ttgcagttga
    17521 tatgagattg tatgctaaag aaaaagcaaa tgatttggtg gatttaatat ctgaatttaa
    17581 agctactata aaagatgttg ctgacaaaaa cccagtgatg atgccaggct atactcatct
    17641 tcaaagggca caagtagtga catttaagca tcacttaatg gcatattata gtatgtttga
    17701 tagagatgag aaaagaataa aaaatgctat agaaatatta gatgaatcac ctttaggctg
    17761 tggagcttta gcaggaacta cgcatgatat agatagaagt ataacttgtg aacaattagg
    17821 atttaaaaaa gtagtagata attttatgga tggagtaagt gatagagatt atttactaga
    17881 gctaatgagt gatttttcaa ttataatgat gcacttaagt agattaagtg aagagttgat
    17941 actttggagt tctcaagaat ttggatttgt tgagatagat gatttatata caactggaag
    18001 tagtataatg cctcagaaga aaaatccaga tggtgcagag cttataagag gtaagactgg
    18061 tagagtatat ggaaacttat ttggtctttt tacagtaatg aaaggaatac cattagcata
    18121 taataaagac atgcaagaag ataaagaagg ttttttcgat agtgtacata ctcttgaaat
    18181 gtgtatacaa ataatggata gaatgatagc cacactaaag gtaaatgagg ataaaatgaa
    18241 acaagcagta aaaaatggtt ttttaaatgc aacagaagtt gcggattatt tggttaaaaa
    18301 taatgttgct tttagagatg cacatggtat agtaggaagt atcgttatat attgtgaaga
    18361 taataaaaaa gcaatagaag atttaacatt agaagaatta cataagtttt cagatgcttt
    18421 taaggaagat atttatgatt ttatcgacta tgagagtata ttaaataagg gaataaaaaa
    18481 gaatttaaaa taatgtattt atttttaaaa gctgtaaatt tgaatgttat catttaaatt
    18541 tacagctttt tattgtgtaa gattttatta tataaaagta agaaacttaa aaggagtagt
    18601 atttaaaata aatacaaata aaaaattatt attaaaatga t
//
LOCUS       DAMJYC010000081        18594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJYC010000081 DAMJYC010000000
VERSION     DAMJYC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.082"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..321
                     /locus_tag="PC472_002913"
     CDS             <1..321
                     /locus_tag="PC472_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2728222.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="PC472_002914"
     CDS             337..1920
                     /locus_tag="PC472_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2728223.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="PC472_002915"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="PC472_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2728224.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DLRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3421..4773
                     /locus_tag="PC472_002916"
     CDS             3421..4773
                     /locus_tag="PC472_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2728225.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4965..6074
                     /locus_tag="PC472_002917"
     CDS             4965..6074
                     /locus_tag="PC472_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2728226.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6357..7748
                     /gene="hydA"
                     /locus_tag="PC472_002918"
     CDS             6357..7748
                     /gene="hydA"
                     /locus_tag="PC472_002918"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2728227.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7742..10303
                     /gene="xdh"
                     /locus_tag="PC472_002919"
     CDS             7742..10303
                     /gene="xdh"
                     /locus_tag="PC472_002919"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2728228.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10455..11801
                     /gene="rpoN"
                     /locus_tag="PC472_002920"
     CDS             10455..11801
                     /gene="rpoN"
                     /locus_tag="PC472_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2728229.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12583..13605
                     /locus_tag="PC472_002921"
     CDS             12583..13605
                     /locus_tag="PC472_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2728230.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13665..14672
                     /gene="gap"
                     /locus_tag="PC472_002922"
     CDS             13665..14672
                     /gene="gap"
                     /locus_tag="PC472_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2728231.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14894..16096
                     /locus_tag="PC472_002923"
     CDS             14894..16096
                     /locus_tag="PC472_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2728232.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16122..16865
                     /locus_tag="PC472_002924"
     CDS             16122..16865
                     /locus_tag="PC472_002924"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2728233.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16874..18406
                     /locus_tag="PC472_002925"
     CDS             16874..18406
                     /locus_tag="PC472_002925"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2728234.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7442 a         2150 c         3747 g         5255 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtttag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aggaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acctaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta
     3301 agaaacaaga atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag
     3361 tatataaata aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa
     3421 atgtctcgag ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa
     3481 tcttgggtat tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct
     3541 atgatgatag gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct
     3601 tgcttgttta tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta
     3661 ggtgctaaaa taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca
     3721 atagcctcat catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata
     3781 gtagctggat tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc
     3841 cctagtgtag ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg
     3901 ataaaatgga taactgacaa caaattagaa gcggttgaac caaactcatt agcattagct
     3961 ttagcagtat tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagc
     4021 acagctatat tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca
     4081 gattttagtg cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga
     4141 atgcctagat ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg
     4201 acagaatcag ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt
     4261 aaaaatatta aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc
     4321 aattcatttc caataactcc attcggtcaa aatacaggaa tagtaggtct tacaggtata
     4381 aaatcaagat ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca
     4441 aaatttgcag caataatagg agcaatacct aagcctgttc ttggaggcgt aggatttgca
     4501 atgtttggaa tggtaatggt tggaggagta aaaactttaa gcaaagtgaa atttgatgga
     4561 actaaaaatg gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat
     4621 cctgatttct atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca
     4681 acaggtagtt tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa
     4741 gaaaaaaata ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg
     4801 acttaaagtc attaagtatt aataaaaata attcagagat attaataatt caacaaaact
     4861 atcttatatt ttaaataata taaaaaagta aataaataac aaatacaaat aaaagaatac
     4921 aatctaaata ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag
     4981 ttaatttata tggaatggaa tttttaaatc caattatgac agcggcagga cctggagcaa
     5041 aagatggtgc tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa
     5101 ctatatctgt attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt
     5161 ttttaaacac agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca
     5221 aaaaggtaaa agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa
     5281 tagctgaggt tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt
     5341 atgtaggtac agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag
     5401 atgtaccagt atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg
     5461 ttgaagaagc aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa
     5521 tagatgtaaa tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag
     5581 gtgcaccaat aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata
     5641 taccaataat cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg
     5701 ctggagcaag tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg
     5761 gaaaaattgc taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg
     5821 aaataaaagg actaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag
     5881 ctccagtagt ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat
     5941 acgaagcttt agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg
     6001 gattatgtgt aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag
     6061 ctatgaatga gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag
     6121 gataataaaa tatatcagat aaacaaacac acattatgca ttaataatac agtctaaagt
     6181 taataatttt attatattga tttaactaaa tattagttta gctgaatatt aatttaatta
     6241 ggtattggtt taatcttaat atagatttac tgcgtataag tgaactaact agattacttt
     6301 aagttaattt tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg
     6361 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
     6421 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
     6481 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac acacactttg
     6541 acttagatac aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta
     6601 tagtaggagg aactactact atattggatt ttgctacaca agctaaagga tgttctttga
     6661 aagacggatt agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct
     6721 ttcatatggc tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga
     6781 aagagggagt atcttcattt aaactgtata tggcgtataa aggaagcctt caagttgatg
     6841 atggtgtaat ctttgaagca ttgagaaaag cagaagagat aggagggatt attggtttcc
     6901 attgtgaaaa tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt
     6961 tatctccaaa atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa
     7021 gacttatgaa gatagctgaa gttgcaaact ctaaagtata tgtagttcac ttaagctgta
     7081 aagaatcctt agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa
     7141 cttgtcctca ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg
     7201 ctaagtatgt aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg
     7261 ctattttaga taatcaaata gacactatag gaacagacca ttgttctttt aattataagg
     7321 gacagaagga acttgggata aacgatttca gtaagatacc aaatggttct ccaggtgtag
     7381 aacataggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
     7441 agatggtaga agtaacatca actaatgcag caaagttgtt tggaatgtat cctaataaag
     7501 gaactataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca
     7561 aaatagaagc aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
     7621 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
     7681 taatttgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
     7741 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
     7801 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
     7861 ttgtggaaca tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac
     7921 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
     7981 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
     8041 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
     8101 aaagaaagct ttaaaaggta atatatgtag atgtacaggg tatgtaaaaa taattaaagc
     8161 tgtcgattta gcggcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
     8221 gggtctagta ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc
     8281 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagagctaa
     8341 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt
     8401 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtagggc acttaaagaa
     8461 ggattgggat actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
     8521 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
     8581 ttatgaggaa ttaacaccat taagtactac agcagatgca cttagagaag atgctccaaa
     8641 gatacatgag acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga
     8701 aaagatagaa aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca
     8761 cgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
     8821 ctattcgaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttagggtt
     8881 accatatgag aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga
     8941 agacatgagt gttcaacacc atgcagcctt acttgcatac catacaaaga gatttgtaaa
     9001 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
     9061 ggaatttacg acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat
     9121 atctgacaca ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca
     9181 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
     9241 taaccctcct ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga
     9301 atgtaatcta aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa
     9361 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
     9421 aatggcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
     9481 agcttgtgct atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag
     9541 attggtgata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
     9601 gcttggtact gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
     9661 agttcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
     9721 acagacagta tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt
     9781 agactctggc aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag
     9841 tattacagat aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata
     9901 tgcaacacaa gttgtaattc tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca
     9961 tgatgttgga agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt
    10021 tatgggtctt ggatatgctc taaccgaaga ttacccagta gttaactcta ttcctacagc
    10081 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    10141 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    10201 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    10261 tgagttacca ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt
    10321 aaataaaaaa atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta
    10381 aatgatagaa tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt
    10441 gagaggtgag cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa
    10501 taatgactac acaacttaag caatcattat caatattaaa tatgagtaag ttagagttag
    10561 aagaagagat aaaaagagaa gcagagaata acccattagt tgaagtcgag aaaagtgggg
    10621 agataaattg ggaagagtat ataaaagaca tggataggtc aagacgttta gatagaacag
    10681 agataagcta taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa
    10741 atttatatga agatttaaaa tttcagataa gcctgtataa acttacagac aaagaattgg
    10801 aagtctgtga gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa
    10861 aagtaattgt tgatacctta aagatagatg aagaactttt tgaaaaatgt ttaattagtg
    10921 ttcagcagtt ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac
    10981 aaatggctag tttaggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa
    11041 atttaattgc aaataataaa tacaaagaga taagtaaaaa gtacaatatg tcattacaaa
    11101 aatgtgttga actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt
    11161 cagtagagaa aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat
    11221 ttattgtata tttaaatgag aaagattctt atcaaataag aatcaatagt tattataaag
    11281 acatattaaa aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa
    11341 attcagcaac aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg
    11401 cagaagagat agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac
    11461 ctttgaaaat gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag
    11521 gtgtaaatgg taaatatatg ctaacacctt ttggcgtata tgaatttagg tactttttca
    11581 gtagcgccat tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta
    11641 taaaggaaac aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcta
    11701 agttattaaa agaaaaagga atcaatgttg caagaaggac agttgccaaa tatcgtgaag
    11761 aattaggaat cccatcttca agtaagagaa aagaatacta ataagtaaaa gacaaatatc
    11821 agtatggtaa ggggaaaagg ctaattaaga gatgaatgta gattgggcta aaaattttat
    11881 aaagtaaaat aatataatat gaaaaagtat taaaatgcta taaggtatta aaaaactggg
    11941 gaacattaaa acattaagat agcaaagagc ataaaaaact aaagaagatt aaaacattaa
    12001 agaacattag agctttgagg aatattagaa ctttaaagaa tattaaaatt tataaaatat
    12061 aaaaatacta aaaagaattg aaaaactggg gaacattaaa atattgtaaa atgctaaaaa
    12121 actagaaaac ataattaaat aagtgcttat aaagaatgat taaattaaat aagtgtttgt
    12181 aaagaataat tagatattag aaaaagaaga atacctagat aaataaacta ttattaaatt
    12241 taataatcac aaaaggaaaa cacgtagggt ataaataaaa atcttaacta gataaaagta
    12301 aacttagttg tagataaaaa taactagaaa taaaattatt tatagtaata aatattagaa
    12361 tgaaaatata aaaaaatata tataaaaagc cttatataaa aagaaaaaat actgtgattt
    12421 gaaaatggtg gagaaatatt ttataaaata aaaaaataaa aaagtttttg aaaaactatt
    12481 gaaatctata cgcaaatcct ataaaataaa aatatcttgt gggacttaaa taaacactag
    12541 ggacaaaaaa agtcccgaaa ggaaagcaat aaggagtatg ttatgaagaa cttattagaa
    12601 attcaaaaga aattgattcc acaggcaatt gaacttatgg aaagaagata ctctatttta
    12661 agacaaatat ctttaagtga accaataggt aggagaacat tatcaaatgt attagagatt
    12721 agtgaaagaa taatcaggtc agaaactgag tttttaaaag aacaaggact tatagacgta
    12781 gctgtatctg ggatgactat cactaaagaa ggttctgatt tgttagataa acttaaggac
    12841 atcatgagtg acatcatggg attgtctaag ttacaagata gagtcagaga aaagcttgga
    12901 ataaaaaggg taatattagt tcctggaagc tgtgatgaaa atggaagttt actaaaagat
    12961 gtaactagat atggatgcga atatttctta ggcattttaa aagatggaga tatcgtttca
    13021 ataacaggag gaagcactat gttggagttt tcaaacgtta ttaaaactga taagagatat
    13081 ccaaactcta caatagttcc agctagagga agtatgggaa ctgatgtaga gatgcaatct
    13141 aacaatatag ttgcttcaat aagtaagaaa ttacattcta attgcaaact tttacatata
    13201 ccagatgagt tggatgaaag tgcaatgaaa actcttagcc aagtgccaga gattaagaca
    13261 actttggagt atattaaaag aacagacatt ttagttttta gtataggtag agcagatgtc
    13321 atggcaaaga gaagaaaact tccagaagac aaggttgatg agatattatc taaaaatgca
    13381 gttggagaag catttggata ttatttcaac aaagatggag aaatagttta caaactgaac
    13441 actgttggaa ttgatttaga aacagttaaa caagtaaaag aaactattgc aatctttgca
    13501 gggactgaaa aagtagaagc cttattagct atatcaaata tgcataaaaa catggttctt
    13561 gtaacagatg aagaaagtgc ttataagata ttgtcattat tataaaagtc aagacctagc
    13621 acagagctag aaaacataca tttactttat aggaggtagc gaaaatggtt aaagtagcta
    13681 taaatggatt tggaagaata ggaagattag cattaagaaa aatgatggag caacaagata
    13741 agttcgaagt tgttgcaata aacgacttaa cagacgctaa aatgttagct catttattta
    13801 aatatgatac agctcaagga agatttaacg gagaaataga agttaaagaa ggtgcttttg
    13861 tagtaaacgg aaaagaaata aaagtaacag cagaaagaaa tcctgctgac ctaccatggg
    13921 ctgaattagg agtagatata gtattagaat gtacaggatt ctttacatct aaagataaag
    13981 ctgaagctca tatacaagca ggggctaaga aagttgttat atctgcacca gctactggag
    14041 atttaaaaac aatagtattc aacacaaaca gtgacatatt agatggttct gaaacagtta
    14101 tatcaggagc ttcttgtact actaactgtt tagctccaat ggctaaagta ttaaatgata
    14161 aatacggtat agaaaaaggc ttaatgacta ctatacatgc atacactaat gaccaaaata
    14221 ctttagatgg tcctcatcca aaaggtgatt taagaagagc tagagctgct gctggaaaca
    14281 tagttccaaa cactacagga gctgcaaaag caataggctt agtaatacct tcattaaaag
    14341 gaaaattaga tggggcagca caaagagtac cagttgtaac tggttcaata actgaattag
    14401 tttgtacatt aggaaaaaat gttacagttg aagaaataaa tgcagctatg aaagaagctt
    14461 caaacgaatc tttcggatat actgaagaaa tgttagtatc ttctgacata atcggaataa
    14521 gctacggttc attatttgac gctactcaaa caaaagttat ggaagtagat ggaaaacaat
    14581 tagttaaagt tgtttcttgg tatgataatg aaatgtctta tacttctcaa ttaataagaa
    14641 ctttaggata ctttgcacaa ttagcaaaat agtcagtttt gagaaaatag aaaagtagat
    14701 tgacaaagtc cgagtttgta gtttaatact acggactcag gacttttttt aagaaaaaat
    14761 atataaaata cacctaaata atgttacata ctatatataa aaatatgata atatgatata
    14821 tttaataggg taaaagtatg tactatatta tacttggcaa taaaaaatta ctttattaaa
    14881 aggagagata tttatgtcaa tgcttaacaa aaaaactata gaagatattg atgtgtgcgg
    14941 aaaaaaagta ttagtaagat gtgactttaa tgtgccatta caagatggtg taattactga
    15001 tgaaaataga ttgaacggag ctttaccaac tattcaatat ttaatttcta aaggggcaaa
    15061 agttatatta tgctctcact taggaaagcc aaaaggagaa gctaaaccag aattatcttt
    15121 agcaccagta gcaaagagat tatctgagat gttaggaaaa gaagttgtat ttgcagcaga
    15181 cgataatgtt gtaggagaaa atgcaaagaa agcaactgaa aaaatggaaa acggagatgt
    15241 agtattatta gaaaatacta gatatagaaa agaagaaact aaaaacgaag agaatttctc
    15301 taaagaacta gcatcacttg ctgaaatatt tgtaaatgat gcatttggaa ctgctcatag
    15361 agcacattgc tcaacagttg gtgcaggcga gtttttacaa gagagagttt gtggatactt
    15421 aattcaaaaa gaattaaaat tcttaggaga agctgttgca aaccctgtaa gaccatttac
    15481 agctatactt ggaggagcta aagtttctga taaattagct gttataaacg aattattaga
    15541 aaaagttgat aatctaataa taggtggagg aatggcttat acattcttaa aagctcaggg
    15601 ttatgaagta ggaacttctt tacttgaaat agataaagtt gaatatgcta aagaaatgat
    15661 ggaaaaagct aagaataagg gtgttaactt attattacca gtagacgttg ttatggcaga
    15721 ccactttgca ccagatgcta ctccaatagt aacagaagat gctaatgtaa aagaagatta
    15781 catgggacta gatatgggac ctaagactat agcaaacttt gtaaaaacta taaaagaatc
    15841 aaaaactgta gtatggaatg ggccaatggg agtatttgaa tttgaaaact ttgcaaatgg
    15901 tacattatca gtagctagag ctatggcaga gttgactgat gcaactactg taattggcgg
    15961 tggagatagt gcagcagcag ttaatcaatt aggatttggt gacaagatga ctcacgtatc
    16021 aacaggtgga ggagcatcac tagaattttt agaaggtaaa gaattaccag gtattgcagc
    16081 attagataat aaataaacta atatatagag gagattgtaa aatgagaaaa cctataattg
    16141 caggaaactg gaaaatgcat aaaacaataa aagaagcttt agaatttgta aatgaagtta
    16201 aagataaagt aaattctgat aaagtagaag ctgttatatg tgcaccattt actttattaa
    16261 aggatttaaa agaagctact aagggtacaa atataaagat aggtgctcaa aatatgcact
    16321 ttgaagaaaa aggagcattt acaggagaag tttcacctct tatgttaaaa gaaatagata
    16381 tggactatgt tgtaatagga cattcagaaa gaagacaata cttcaatgaa actgatgaaa
    16441 ctgtaaacaa gaaagtttta aaagcattag aagtaggaat agacccaata ttatgtgttg
    16501 gagaaacttt agaacaaaga gaagctggaa aaactaaaga tgtttgtaaa gttcaagttg
    16561 aaaaagcttt agaaaatgtt cttaaagatg atttagcaaa agtagttgtt gcatatgagc
    16621 caatctgggc tattggaact ggtaaaactg caactgctga agatgctaat gacgttatat
    16681 cttatataag agaagttata aaaggattat atggagaatt agctaatgaa gttagaatac
    16741 aatacggtgg aagtgttaaa ccttcaaatg tagccgaaat aatgggtcaa agtgatatag
    16801 atggagcttt agtaggtgga gctagtttag ctagtaatga ttacctagac cttgttaatt
    16861 tctaaggagt gaaatgatga aaaaaccagt tgctttaatt attatggacg ggtttggata
    16921 caataaggat gttaagggaa atgcaattgc tgagtccaaa actcctaatt tagatagaat
    16981 aaaaaaagag tatccaaaca cattgataaa tgctagtggt cttgatgttg gtctacctga
    17041 tggtcaaatg ggaaattcag aagttggaca cactaacata ggtgcaggta gaatagtata
    17101 tcaagattta actagaataa caaaatctat aaaagatggt gactttttta ctaataaagt
    17161 tttatgtgaa gcaatggata atgcaaaaga aaactctctt catgtaatgg gacttttgtc
    17221 tgatggagga gtacattcac atatagacca tcttaaggct ataatcaaaa tggcaaaaga
    17281 taaaggcgta caaaaggtgt atgttcacgc atttactgat ggtagagata ctgaccctca
    17341 aagtgcatta gaatatgcca aagaagtaca agctagtatg gatgaaattg gagtaggtga
    17401 atttgctaca gtttctggta gatactacgc tatggataga gataagagat gggaaagagt
    17461 agaacttgca tacaatgcca tggttagagg tattggtgaa aaagcaaatt ctattgagga
    17521 agctattcaa aactcttatg atgatggaaa aaatgatgag tttataatgc caacagttat
    17581 tatgaaagat gataaaccag ttggaagtat aaaggaaaat gattcaatta tattcttcaa
    17641 ctttagacca gatagagcta gacaaataac tagagcttta gtttgtgaag agtttgatgg
    17701 atttaaaaga gaagatatta aaaacttctt tgtatgctta acagagtacg atataactat
    17761 agagaatgtg catatagcat ttggacctca atctttagcc aacactctag gtgagtatct
    17821 agctaaaaat ggaaaaactc aacttagagc tgctgaaact gaaaagtatg cacatgtaac
    17881 tttcttcttc aatggtggag tagaagagcc aaataaaggt gaagaaagac tattgatacc
    17941 ttctccaaag gttgcaactt atgatttaaa accagaaatg tcagcatatg aattgacaga
    18001 taaagcttta gataaattag gagaagataa gtttgacttt atagtattaa actttgcaaa
    18061 tcctgatatg gtaggtcaca caggaagtat agaagcggct ataaaggctg ttgaaactgt
    18121 agatacttgt gtaggaagat tgatagataa aatagtagag ttaggtggaa gtgcaataat
    18181 aactgctgac cacggaaatg ctgaatatat gttagaccca gaaacaggaa aaactgtaac
    18241 ggctcactct attaatccag taccatttat tgtagttggt caagaatatg aaagtgcaaa
    18301 attattggat ggaggaagat tatctgatat agcacctact attttagata tgatgaagtt
    18361 agaaaagcca gaagaaatga ctggtcattc acttatttca aaatagtcga ttctgataag
    18421 tagataataa tatataaact acaatataaa tttaaaaata atgcagttta ggtttatttt
    18481 gtaaagttta gactttatga cataaggttt aaattttgta agttctggat attgtaaggt
    18541 gtagatgaag atatatttta taaaatttac aagatagaca atttataaaa taaa
//
LOCUS       DAMJYC010000082        17900 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJYC010000082 DAMJYC010000000
VERSION     DAMJYC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.084"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            176..511
                     /locus_tag="PC472_002926"
     CDS             176..511
                     /locus_tag="PC472_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728235.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            648..1925
                     /locus_tag="PC472_002927"
     CDS             648..1925
                     /locus_tag="PC472_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2728236.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2046..3434
                     /locus_tag="PC472_002928"
     CDS             2046..3434
                     /locus_tag="PC472_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2728237.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3805..4518
                     /locus_tag="PC472_002929"
     CDS             3805..4518
                     /locus_tag="PC472_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2728238.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4636..5505
                     /locus_tag="PC472_002930"
     CDS             4636..5505
                     /locus_tag="PC472_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2728239.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5660..6805)
                     /locus_tag="PC472_002931"
     CDS             complement(5660..6805)
                     /locus_tag="PC472_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2728240.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7138..7914)
                     /locus_tag="PC472_002932"
     CDS             complement(7138..7914)
                     /locus_tag="PC472_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2728241.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8496..9470
                     /locus_tag="PC472_002933"
     CDS             8496..9470
                     /locus_tag="PC472_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2728242.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9568..9873
                     /locus_tag="PC472_002934"
     CDS             9568..9873
                     /locus_tag="PC472_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2728243.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            10029..11189
                     /locus_tag="PC472_002935"
     CDS             10029..11189
                     /locus_tag="PC472_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2728244.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11239..12141
                     /locus_tag="PC472_002936"
     CDS             11239..12141
                     /locus_tag="PC472_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2728245.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12221..13114
                     /locus_tag="PC472_002937"
     CDS             12221..13114
                     /locus_tag="PC472_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2728246.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13167..13348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13576..14706
                     /locus_tag="PC472_002938"
     CDS             13576..14706
                     /locus_tag="PC472_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2728247.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14778..15803
                     /locus_tag="PC472_002939"
     CDS             14778..15803
                     /locus_tag="PC472_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2728248.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15806..17002
                     /locus_tag="PC472_002940"
     CDS             15806..17002
                     /locus_tag="PC472_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728249.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17130..17855)
                     /locus_tag="PC472_002941"
     CDS             complement(17130..17855)
                     /locus_tag="PC472_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2728250.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6874 a         1986 c         3115 g         5925 t
ORIGIN      
        1 tgttatgtat ttacaaaact gatatattaa ttacataaaa tctaaaatag tttgtaatag
       61 gagtatatat aataaaaatt ttaattaaat aaagctaggt atatttataa tattaataaa
      121 tatattgcta taaaagtttg tttaataaat taaataaaga aaagaggaga ataaaatgta
      181 caacatagat gatatgatat ataattgtcc agtagaagct ttatcaaata ttttaggtaa
      241 aaaatgggtg gctataatta tatggaaaat acaagaagat aaaaaacgat ttggagagct
      301 acaaagagct ataccagact gtagtaagaa aatgttagtt caacaactag attttttaat
      361 tgaacagggg attatagtca accaaaaaaa gttggttaat aatgttgtag agtcaacata
      421 ttttttaagt gaatcaggat tacgattgct tcctgtcatg gaaaaaatga ttatgtggag
      481 caataaaaat ttaaattgtg ataaatacta aaatttactc tgtaagtaat gaaaatttaa
      541 ttaagctatg agtagaaaaa tgtattaaaa ttacataatt ttttagttag attttaattt
      601 agaaatggga ttgtaatatt ggaaataaaa taaggaggaa ttttaatttg caaaatgtaa
      661 atttattttt agtatttata gaaggtatag tatcattctt ttcaccatgt atactgccaa
      721 ttttgcctat ttatttgagt atattgtcaa atagtagtgt agaaaattta aaagaaggaa
      781 agacgagttt tataggaagt tctttattta aaaatactat attttttgct cttggaatct
      841 caacaacatt ctttatattg ggttcatcag taaaagtatt gagtatgttt tttaatgaaa
      901 ataaagattt aataatgttt attggtggga ttataattat tatcatggga ttattttaca
      961 tgggaataat taaatcttca atattgaata gagaaaaaag atttaatgtt aagtttaaag
     1021 aaatgaaagc aataacagct tttatattag gttttacgtt tagttttgga tggacacctt
     1081 gtattggtcc aatattggca tctgtattag tcatggtttc aagttcaagt aatcatctta
     1141 gtgccaatct tttaatagca gtatatacta ttggttttat tttgccattc ataattacag
     1201 ctatgtttta cagtaaacta tttaaaacta tagataagat aaaatctaac atggaaatta
     1261 ttaaaaaaat aggtgggatt atacttatag tatcaggaat attaatgatg gtaaatggat
     1321 ttggaagtat aagcaaacac tttaatacgt cacaaaatag taaaattgaa agcaagcaag
     1381 aagaaaataa gcgagaaaat tctactgata aagaagaaaa ttctgatggt aatgatagtc
     1441 aaaaagactc taataatgac aataatgata aagggtctaa tgatgaagat agaataaagt
     1501 ctattgattt tactttaaca gaccagtatg gtaaaacaca taaattaagt gattatgaag
     1561 gcaaagttgt attcttaaat ttctgggcta catggtgtcc tccatgtaaa gaagaaatgc
     1621 cttatataga acaactgtat aaagactata ataaaaataa tgatgatgta gtaattttag
     1681 gagtagcttc acctaattta ggtagagaag gttcaaggga acatgtagtt aacttcttaa
     1741 aagaccaagg ttatacattc cctgttgtac ttgatgaaga tggagcatta gcatatcaat
     1801 atggaattaa tgcttttcca actacattta taattgacaa ggaaggatat gtaactcagt
     1861 atattccagg agctatggat aaggcaacta tggcatcatt tattgaaaat cagagaaata
     1921 aataaaagta tttaagtata agtttataaa tcaatttaag gttacaaaaa agtgcctaat
     1981 tgtaccacct aaataatctt gttatagtaa aagtataaac aatacaagaa agggttgaat
     2041 tttatatgaa aaaaacattt gatgctataa taataggatt tggaaaaggt ggaaaaacat
     2101 tagcagggga tttggcaaac aggggattaa aagtagcttt aatagaaaag tcaaacaaga
     2161 tgtatggtgg aacatgtgta aatgtggcat gtataccaac aaaaagctta gaaaattcag
     2221 caaacagcgt aaaaacaaag aatataaatt cttgggatga agtacaagct gaatatgaga
     2281 aagctataga taaaaaagaa actttaatta caaaactcag agaagctaac tataataaat
     2341 taaattcaaa tgagaatgta actatattca caggaatggg aactttcatt gatgaaaaaa
     2401 cagttcaagt caaaactgaa aatgaaattt atgagttagt agcagataat atatttatta
     2461 atactggttc aagaccattt atacctaata ttaagggaat tgaaaataag aatatagtat
     2521 atgatagtga gtctcttatg aatctaagag ttttacctaa aaaaatgaca attataggag
     2581 ctggatttat aggtttagaa tttgctggta tttatagttc atttggagca gaagttacaa
     2641 tattaaactc taataatggc attttaccaa atgaagatgt tgaggattca gaagaaataa
     2701 taaaattact tgcaaagaga aatgtaaaaa ttgttaataa tgcaaatata aaagagatta
     2761 aagaagtttc tgaattagca atagtagaat atgaagtaga tggtaaatct aaagaattaa
     2821 caagtaatat gatacttgta gctacaggaa gaaaagcaaa cactgaaggg ctagggctag
     2881 aaaatgcagg tatagaatta aatgaaagag gatttattaa agtctcagaa actcttaaga
     2941 ctaataaaga gcatatatgg gctataggag atataaatgg aggtccacaa tttacttata
     3001 tttcattgga tgattatcgc atagttatta atcagttatt tggagataag actagaacaa
     3061 ctaatgatag aaagaatatc ccaaattcta tatttataag tccagcattt tcaagagttg
     3121 gtcttaatgt taagcaagct aaagaaaagg gatatgaagt attagtggca aagatgccag
     3181 tagaagcaat tccaagagct aaacagattg gcaaggcaga tgggtttata aaaatagtga
     3241 tagataaaaa atccaataag atacttggag ctagtatgat atgtgaaaat tcaagtgaga
     3301 ttatccatct tattcaatta gctgtagatt tagaagtaga gtatacatat ttaagagacc
     3361 gtgtttatgc acatccaact atgacagaag ctcttaatga tattctatct cctaatatga
     3421 taaaagaagt ataacataag tttaatatat aaacctaaaa ttatcattat aagtagattt
     3481 tagaaagaga ctatctcaaa aaatagagat aagtctcttt tttactgttt aaataataaa
     3541 aatatatttg attttataaa agaagattat tttatgagct aaaaagttta tttagaaaca
     3601 ctacctattt atgttataaa gttataaata taatatagaa tctattattc tgtattatta
     3661 agtggggtta agcgttataa ttaattataa atattgaata atctaaatgg ataagataat
     3721 ggtattaaaa attatcttaa aaggtgaaat aatctgtatt taatgtagta cagttatatc
     3781 tttaaagcaa ggtggtgaaa tgatataaat aagatatcta aaagagggtt tgatgatagt
     3841 gataaaagat ggttttcaag taaagaatta acaagactta taaaggctaa agaagaaata
     3901 gaatggttat taaataggga ttacaaaata gacccagtcg ttacttttgt tggtgataga
     3961 tatcagtttt ctataagaca aagagatgca ttaaaaagag cagtctgtac tgatgaaaaa
     4021 aatattataa gacaatcaaa gaggctttct ctagataaaa tcaaagaaga taccgtaaat
     4081 atagatggct ttaatttgat aatatccata gaggtaggat tatctggtgg tactctagta
     4141 ataggaagtg atggcaatat aagagattta gcaggtctta ggggaaccta taagattata
     4201 gataaaacag aagaagcttt gaatctgata ggtaaatttt ttaataaata caatgcacag
     4261 aatcttaaat tttatttaga tgctccagtt tctaattcag gaaatttaaa gtataggata
     4321 ttagagcatg ccaaaacttg gggaattgaa actgaagtag aattagtaaa aaatgcagat
     4381 gttgtattgg aaaaattaga tagagtagtt agtagtgatg cagtcatagt tgacaagtgt
     4441 ataagttatt ttaatgtggc tagaggtata attgaagagt atattaaaga atgtaatatc
     4501 ataaatttaa ataaatagat aaatataggt gagaatatat aagtatatgt agcaatattc
     4561 ttagtaatac aagtaaagtt tgaaaatagg agaacgaata catatcgaat ttcataaaat
     4621 gttgtaaaaa gacatatgag aggatggata tgtgtgaaat taactaaact aaatattaaa
     4681 aaatatagag cacctatata taaatatgct ttagcaaata taaatttgag gtctgctaaa
     4741 tctaccaatt caagtatcat aacagtaata cctcagggag ccaaaatgga agtattggat
     4801 gaggaagatg actggattaa ggtgatgtac aattctcaag aaggatatgt gtataaggag
     4861 ttggtatcag taagcgaata tgcttggagt aacttaaact taagagaaga taaatctaca
     4921 acttccaata taattacagt aattcctgaa aaatctaggg ttgaagtcct tcaagtagat
     4981 ggagattgga gtaaggttgt atatgatgat aaaataggat atgtttttaa ttatttttta
     5041 tcaattgatg ggaataaacc aaatgaatta gattataagt atttttatac tgacatgatt
     5101 aaatttgtta acgaaaataa tattaaaagt actagcgatt atctaattgt aacagacctt
     5161 agaaataaat atacttatat ttttaagaaa gataatggag gatggggaca actttacaag
     5221 tggcaatgta ctataggcaa gcctgagact ccaactataa caggtatatt ttatataagt
     5281 ggaagaaaac cttcttttgg aacggatgag tattcagtta agtatgctac tagaattaaa
     5341 ggtggatatt actatcattc tgtactatat gattcaacag gaagttatat aatagatggc
     5401 agacttggtg aagctcttag tcatggatgt ataagattaa gtacggaaaa tgcaaagtgg
     5461 atatatgata atataccaga tacaacaaca gttataattc attaagaaaa attttgagtt
     5521 ttttattggg ataatgattc ttaaattaat aggattatat attaacttct cataaagagt
     5581 actatttaaa aagtgtaata agagagtgct attaacaaaa caaagatatt ttgaggcact
     5641 ctcttataaa tatacataat taagatattt tcataaacaa ttctataatc ttatctttat
     5701 caaagaaaac aggattagca ccagcacaag catctttcat agagttttct gcaagcaatt
     5761 caaaattagg attatcaaca cctacatctt ttaaagattt tgggattcca acctcttcag
     5821 aaagagcttt tatcttatct attacaaagt caacacactc tttatctgat ttattattta
     5881 catccattcc aataacttca gctattggtc ggaatttggc aggggctgat ttagcatttt
     5941 cctcctcaac tattggaagt agcatggcat tacatactcc atgaggtaaa tcatataaac
     6001 ctcctaattg atgagccata gcatgtacat ttccaagacc tgcattacta aaggcaattc
     6061 catttaaaaa acaagcataa cacatttgtt ctctagcttc tatgtcatta ccatttttaa
     6121 cagctcttgg aaggtattta aatatctgct ttatagcata taaggcagtt gaatctgtaa
     6181 catcataagc accattagct actacagctt ctatagcatg agttagtgca tccataccag
     6241 ttgctgctgt taaattagat ggtttgctaa tcattaattc tgggtcattt actgtcattg
     6301 tggctagtga attggtatca accattatca tctttacatg tctgtcttca tcagttataa
     6361 catagtttat tgtaacttca gcagatgtac cagcagtagt agttatagct atgataggaa
     6421 gacatttttt agaagttttg ttgatacctt catagtctct aatatctcct ccattagtgg
     6481 ctaatataga gatagcttta ccacagtctt gtggagaacc acctcctata gttataacga
     6541 agtcgcaatt ttctgatttt aatattttaa gtcctttatt cacattagtt acagttggat
     6601 tttgtttaac ttcatcataa acaacaaagt ctatatctat ctgagataaa atatctgtta
     6661 ctttttttac agtaccattt gaaactagaa atttatcact aacaataaat gcttttttag
     6721 ttcctaatga tttaattgaa tctttagctt cattaagaca tcctttccca agtaaattta
     6781 ttggtggtac ataataaatg ctcataacta aaatctcctt ttattttcat tgatttttat
     6841 gtaaaaatat atatctaaca attttcacta atttactcat aatatatcgt atcttagatt
     6901 gagatagggt tgctggttct tatataagaa cttaattcgt aattacgatt atgattagaa
     6961 tttaacatag tatttttatc atgtcaataa aaatataaaa aatattataa aaaaaacaaa
     7021 gttactgtag ttaaatcagc aactttgttt ttatagtata aatcaaataa atacattagt
     7081 ttaaaacaaa atttttaatt attatgtttt gatatcttcc atatacagaa tattaatcta
     7141 atttattaca atgtcttcat tacgttcttc catcattgat tgaatatttt tctgagcatt
     7201 taagagtaat ttttttatca gttctatttt ttcgtcatta gctctaaata aaggcagtgt
     7261 tacactaaca gcagctctta tacatccatt ttttcttatt ggtacggcaa gacattttag
     7321 gtgttccatt gattcctcac actctgaagc tatttgtgtt ctttgaactt caagaagctg
     7381 gtcgtataaa acatcaaaat ctgtaattga tttttctgta aaacctttta gaccatctgg
     7441 atataagctt attagttttt ccttgtcaaa atcacataaa agagcttttc caagacctgt
     7501 acaattagca ggtatttttt ttccaacata agaaataagt ctaatcggtt ctggagaatc
     7561 tacttttcca atataaagaa cttcattatt gtcaagtata ccaagctgac aagtttcaga
     7621 acaggaatta actatattag tcatttcgtc aattatatag ttaaaaatag ttcttttttc
     7681 aaaaaatgaa gaccctaatg tgtaagaacg tatccctata gaatatttgg atgtgttttt
     7741 atcaatagta atgtattttc tagcattaag tgtatgaact ataggaaaga tgctactttt
     7801 tggagcattg ataacatctg ctatttctgt aagagtatat ccattttcgg attctgctaa
     7861 tgcttctaaa atatctaata tcctagctgt tgggcgatga ttatctgaag tcataaattt
     7921 ccccctcttt ttattaattt aaagtattag aatatgttga ttttagcaca ctatacgata
     7981 ttttacaatg tagaaaataa atatgtaaat ttatatataa ataagctctt gatttagata
     8041 cagatagata tttttttaga ctgtacatta aggaataact aatttccaaa atatacctaa
     8101 agaaagtaag tgatatttag ttatataatt taagtaaata tatgcaatag ttatttttat
     8161 atgtaaatat caaaatatta tgaacttata ttttatctat tttttacact gtataaaaac
     8221 tttatagttt tgaatactat ataattttaa atacgctgta tttttgcata taaggaagtc
     8281 atttgataat atacaaggta ttatcagaaa gtttgaaaaa attttgacaa aaatttattt
     8341 tttttattga ctcataaata ggaaaatgct atcattaatt caaagattcg taattaaaaa
     8401 cttagttctt aaatacgaac cgaaacggcc gttagaatta aagctaaaaa cttaataaga
     8461 gtaattaaat atcatgtttt aggaggatta actttatgat aatggatttt attaaaaaaa
     8521 ttcctgcagg tatgatgata gtgccaatgt ttatagcagc tttcattaat accttttgcc
     8581 cagatattgt acaaataggt tcgtttacga cagcagtatt ttcaagtgtt gggtctgcaa
     8641 ctataattgg ggttcaactg gtatgcttgg gaactcagct tcaatttaga gaaatgccaa
     8701 aagttttaaa gagaggtggt atacttcttg gttctaaatt tgctataggt gctttgatag
     8761 gtatacttat aggaaagcta tttggaatgg atggtgtcct tggtctgaca acattggctg
     8821 ttataagtgc agttacaaat agtaatggta gtatatatct ttcattgatg acttcatatg
     8881 gagatgaaac agattgtgca actatgagtt tacttacttt aaatgatgga ccatttttta
     8941 cattaatagc tcttgggact tcaggtcttg caaatgtacc attgttgtca ttattggctg
     9001 caatagtgcc aatacttgtt ggtatgataa taggaaattt tgacaaaaaa atgaaagagt
     9061 ttttagagcc agggtgtaat ttactcgtac cttttgtagg gtttgcactt ggtagtagca
     9121 taaatcttgc aagtatacta aaaggaggtt tatcaggagt attacttgga ttaatatctg
     9181 tatttgtagg aggtatattt atagtattct gtgataaatt tataggaaaa cgtccaggat
     9241 atgcaggatg ggctgtagct acaactgctg gaaatgcaat tgctgtacct gctgctgttg
     9301 cattagttga cccatcatgg caaccatatg ttgcaacagc tacaacacaa gtggcagccg
     9361 cagtagttgt cactgcaata ttagtacctc ttataactag ttggtgggct aagaagtatg
     9421 gatgtcctaa gttcccaaaa caaaatgagg atggtacaat aacagcttaa tttatattac
     9481 agtgagttgt tggtaaaata ataaattctt taattagatt ttaggatatt aataaaatta
     9541 tattatttat aaaggagagc aagaataatg ataaatgcag tgataattga tgaaaaagat
     9601 aatgttgcag tagctattga agcaatagct aaaggtagtg aaattagttt taaattaaaa
     9661 gacaaaacag taaaaacgat tacagcacta gatgatataa ctatttatca taaattggct
     9721 ataaaagata tggccaaagg tgataaagtt actaaatatg gtgaacatat aggagaagca
     9781 aatcaggaaa taaaaagtgg ccaacatgta catattcata atgttagaag tgttagagag
     9841 gatttggata aacaagttat gactaatata taaaataata tttatagagt tataaaagtt
     9901 gtattcattg cttttagtgt cacatagttt ttaaataggt ttttagctgt ttgattagtg
     9961 agtgtaataa aaattataat atataaaaat tatagaaagt atatagacta ttttaggagg
    10021 aaatcagtat gaaattttat ggatataaaa gaccagatgg aagagttggt ataagaaacc
    10081 acatattaat attgcctacg agtgtgtgtg catcagatac aactagaata atagcatcac
    10141 aagttcaagg ggcagttacc tttaataatc agaatggatg ttcacaagta cattcagacc
    10201 aacaattaac tatggatgtt atggctggtt atgcagcaaa tccaaatgta tatggtgtaa
    10261 tagttgtatc tttgggatgc gaaaattgcc aaaatgattt ggtagttgat gcaataaaag
    10321 agagaacaaa taagccaata aaaactcttg taatacaaga agaacatgga actttaaaaa
    10381 ctatagaaaa agctgttcgt tatgctagag aaatggcaca agaagcttct ttgcttagaa
    10441 aagaagaatt tccaatatct gaacttatac taggaactga atgtggtggg tctgacccaa
    10501 caagtggatt agcagcaaat ccattaatag gtgaattaag tgataaactt gtcgatttag
    10561 gagcaacatc aattttaagt gaaactactg aatttatagg tgcagagcat atactagcta
    10621 ggagagcagt aaatgaagaa gtaaaagaaa aaatattaca tatagtacat agatatgaaa
    10681 attcattaaa attggttgga gaagaagtta gagaaggtaa tccatctcct ggaaatatag
    10741 ctggtggttt aacaagttta gaagaaaaat cacttggttg tatacataaa ggtggtcata
    10801 gacaaataag tgaagtatat gattatgcca aacaaattga taagaaaggt cttgttataa
    10861 tggatacccc tggaaatgat gcttcatctg ttgctggtat ggtagcagga ggagcacaaa
    10921 tagtggtatt tagtacagga agaggaacac catcaggaaa tccaatagca ccagtaataa
    10981 aaataactgg aaataaaata acttttgcaa atatggaaga taatatggat tttgatgcta
    11041 gtccagttat ctatggtcca caaacaatgg aagaattaac agatgatttg ttaaatatgg
    11101 ttgtagatgt agcaaatgga aaacaatcaa aagcagagtc ccttggatat actgaaatgg
    11161 caatagctag agtatgcaac tatgtataat ctttaagtaa gaaaataaat acatagaatt
    11221 ttaataaagg gtgagattat gaagtttgta agttttactg aaggagaaaa tgaatgtaaa
    11281 agaactggag ttttttctaa agatgaatta tttattatag atgttaactc tttaaatctc
    11341 agtagaaatt ttaaggattt aaatgaatta attcaaaatg tatctagtga agatattatt
    11401 aagttgaaaa ctattataaa tgaaagtaca ttctataatt atgatgtttt taaaagagag
    11461 agatgtacgg tacatgttcc tatagaaaaa ccaatacatg atatcttatg tgtgggagtg
    11521 aactataaag accatcttga agaaactcaa actcactttg atgatagttt tgaagaacca
    11581 caaaaaacgg tttattttac aaaacgtgtg acaaaggcaa taggtccaga agatgagata
    11641 gatggtcatt ttgagataaa taatcaacta gactatgaag ttgaacttgg agtagtaata
    11701 ggtaaaacag gtgttaatat tagaaaagaa gaggtagaag attatatctt tggttacact
    11761 gttataaatg atatttctgc tagaaagttg caaaaagaac atgttcaatg gtttagagga
    11821 aaaggattag atacatttac ctctttaggt ccatgtattt tacataaaag tgcagttcct
    11881 tttcctataa aacttaaagt atgtagtagg gtaaatggtg aagaaagaca aagttctaat
    11941 acaggattat tcatatcaga cgtttcagac atagtttcag aactttcaaa aggtatgacc
    12001 ttagaggctg gagacattat agctactgga acaccttctg gagtagggat gggattttca
    12061 cctcctcgat atatgagtag tggagatgta gttgaatgtg agattgagag tattggaatt
    12121 ttaaaaaatt acattaagta actgttttaa tataattaat attaaagcta aatctgatgt
    12181 attaatgaac atattaaaat aaattttagg agtttaaatt atgaaagcag aatttttgac
    12241 accagttgtt acaatatttg atgaagaagg aaatttagat aaagaaggta ataaaaaagt
    12301 gtatgaccat cttatagatg gaggtgtaga tggactagtc ataatgggaa gtactggaga
    12361 gttctttaat atgtctatgg atatgcaaaa agaacttata gatttagtaa ctagtcatgt
    12421 aaataagaga gtaaaagtgt ttataggtac aagtcgtatg tgtataaatg aatctataga
    12481 actttcaaac tatgcacata atagtggagc agatggtgtt atgataataa gcccttatta
    12541 tttctctctt gatgatgata gtattgagtt attttatagt gaaatagcta agaatacaga
    12601 ggctgaaata tatttgtaca attatccaga tagaactggt tatgatttaa gtccagaact
    12661 tacattaaga cttgcaagaa aacataaaaa catagttgga tataaggata cagtaacttt
    12721 gatgggacat accagagatt taataaatac tatgaaaata gagttcccag aatttaaggt
    12781 ttatagtggt tatgatgaaa actttataca taatctaatg tctgggggag caggttgtat
    12841 aggggggtta tcaaatttaa ttccagaagt ttgctccaaa tgggctgaag cttacaataa
    12901 tagagatatg gagaaggtta tagaaattca aaaatatatt aataaagcaa tggattttta
    12961 cttaataacc aatccattta taccagcaat gaaaaaagct atgaatatca gagggttaca
    13021 aataagtgat tatatgactg ctccatttgt aaaaccaaat gaaaaacaag tatgtgaaat
    13081 taaaaaattg atgaatgaat taaatatatg ttaaatcaac cttgcaaaga ggtcaacaat
    13141 aaattacaat tatcttaata aaaattaaat taaaataata ctagggtact atattagttt
    13201 aatagggaaa gtgatgaaaa ttcactacag cccccgctac tgtatacgga tacgaaatca
    13261 aaatccactg aaatttataa aaattttggg aagggtgatg gaggaaaagc cgtgagtcag
    13321 gagacctgcc cagtattata taagttcaat ttagggtgga gattgtaatt tataataaaa
    13381 tgaagtatca attcgttttt gtaaagttga tatatgaact tctccttatt aaattaggag
    13441 aagtttttta ttttattaaa aggttatact tattaaagat gactttagga taactttaat
    13501 aagtatactt taaaaagcat atattattaa agttttttag ccactcataa aaaataagat
    13561 attggagtga aagagatgaa atttaaatca aatgttttaa agcagttttt tttaattgct
    13621 atggtaattt ttatggtagg aggtcttata gggtgtagta aaagtgacaa agagaataaa
    13681 caagctagta ctggtaagaa attagaatta aaatatgcta gtggatttag tgttgaagat
    13741 ttagaagatg gaattaaaaa agtaactgat ggagataatc gagaactgat tttaatccca
    13801 aagaagatta aaattcctga aaaatataaa aatgctaata tagttagaac gcctgtggac
    13861 aatgtattaa tagcatctac aacacaggct tgttctctga gaacaataga tgaactagat
    13921 agtataaaag ccgtaacaac agaagagcag tactggacta ttaaagaaat taaagattta
    13981 atgaaagatg gaaaaataca ttacgttggt tcaaatttag caccagatta tgaaaaaata
    14041 gtagacttaa atcctgactt aactatagtg agtagtggtt tatcagaggc tgatacaaaa
    14101 tttattgaca aattaaaaga attagggctt aattatgttg tagacaatga gtataaagag
    14161 caaaatccat ttggaagaat ggagtggaca agtttaatag ctgcatttta tgataaagaa
    14221 gatatggcta ctagtgaact aaataaaact gtacaaaagg tagaagaagt aagtaaaaaa
    14281 ataaaaaaca aagctaaacc taaagttgta tgggcatctg tatatgaagg aagtgcttat
    14341 atagctccta aagattcata tgtggataat atgataaaaa tggctggtgg aataaatgca
    14401 tgtgcttcaa tagattcaaa tgaagggcga gtaagtattg aacaattgca tgaagttgca
    14461 aaagatgcag atatatttgt atactcatca agttcagatt atgcaccaaa tttagattct
    14521 ataaaatcta gtgcacctgt attggctgat ttggatgttg taaaaaacgg gaacctatgg
    14581 gtgtttgctc cagattatta tcaatcagta gataagacgg acgaattaat agtagattta
    14641 gttgagatgt tccacccagg tactacaggt gaaaaaatca aacattatat aaattatgat
    14701 aaatagtaca gagaggacac aaaaagatag gttttataga ttttagttaa tatagctttg
    14761 ctaagaggag tatacaaatg gattttatca ataaaatgag aataaggaat acaacaatat
    14821 ttatagtatt aattataagt atgatactta cttttttagt gtgtgtagca tttggctctg
    14881 taaaaattga atttaaagaa gtaatagatg ttgtattaag aggtatggat aatacagaac
    14941 attataaaat aatatacaat ataagaatcc ctagagtact tgcaaccatg gtaggtggag
    15001 catgcctagc agtatcaggt attttattac agatattttt taaaaaccca atagttgaac
    15061 cgtatatatt aggagtatct tcaggttcta ctttatcagt agctctcatg attttgggag
    15121 gatacacatt gggatttgag agtgtaaatt caatgagtat atttgtagca gcttttatag
    15181 gagctttaat tgtaatgtct atagtcattt tatttgcaaa taaggtaaag aatataacaa
    15241 ctcttttaat aataggaatt atgaccggat atgtatgtag tggtataaca aatgtgttac
    15301 aatcatttgc aaatagtgat aaattaaaag aatttactat gtggtctatg ggaagttttt
    15361 ctggatttac atgggaaaag gtagaaatac ttataatagt tggtatatta ggtcttttga
    15421 tgactgtatt cataataaaa cctttaaatg cttttttact aggagaagag tatgcaaaaa
    15481 gtattggcat aaatataaga ttttttagag ttttgatagt atttatatct agtatattag
    15541 cagcaatagt atcagcattt gcaggaccta tagggtttat tggacttgca gtgccacaaa
    15601 tttcaaaaat gatatttaaa acatcagata atagagtttt gataccagta gtaatatttt
    15661 taggcagtat agttacagct ctttgtgatt taatatcaag agtaatttta tcaccagtag
    15721 aattaccaat aagtacgata acatctttat taggagctcc aattgtagta atgttattgg
    15781 tgaaaaggaa ggatagggct tagacatgtt gaaaactaat aatttaagtg taggatacaa
    15841 taacaaagtt gttataagta atataaatgt agaagtaaaa aatggagaaa tactatgtct
    15901 tttgggaagt aatggagctg gaaaaactac cctattaagg tccttatcta aacttatatc
    15961 accaataaaa ggtgaaatat atttgaatgg tgtaaacata aattgtatat caagaaaagc
    16021 attatcaaaa aaaatggctt tagtacttac aaatagatta ttaggtgatt taatgactgt
    16081 acaagatata gtcaatatag gaaggtatcc atatacagga ttttttggga gtctatctaa
    16141 gaaagattta ataatggtag atgaagcatt ggagtctgta gatgccttac atttaaagaa
    16201 aagatatttt gatgaactaa gtgatggaga aaaacaaaag gtgttggttg caagggctct
    16261 tgttcaagaa ccagaaataa ttatattaga tgagcctaca actcacttgg atataaaaca
    16321 tagattggaa ctcataaaca tactgaaaaa attaagtaaa gaaaagtcaa tatcagtaat
    16381 actttcattg catgaaatag acatagcatt aaaaagttgt gataaagttg ctcttataaa
    16441 aaataataaa gtcatagctt atggtcagcc tgaagatgta gtagatgagg atattataaa
    16501 ttcgctttat gaattggatg ataaaaactt taatagtctt ttaggttcag tagaaattag
    16561 taataaatct aaaaatgaag tttttataat tggtggtggg ggtaaagcaa caccaattta
    16621 tagagctttt actaaaaaag gtataggtct gtattctgga ataatacatg aaaatgatat
    16681 tgattatgaa attggtagaa ctatggggat aaagatgttt acagaaaatc catttgagcc
    16741 tataagtgat gagagtttcg atttggctat tagaaattta aatgattcca agattataat
    16801 agatacagga tttagtgtag gagagacaaa taagaggaat atagatatta taaaagaggc
    16861 tttaaaattg gataaaaaag tttattcttt tagaaataga gatgagagta agaagtatta
    16921 tgacagtttg gatagtaaga tagaacatat agataaagtt tctcaaataa tcaatagtgc
    16981 agatataaat aatttattat aatttatgaa ataaaatgta cttatgtgct atagaaaaaa
    17041 gagtatagat tataattttt ttgaaattac aatctatact cttttgttat tttgtttatg
    17101 tatttatata tttataaaac tttagcattt tatagataac caatagcttc ctctctagtt
    17161 aaaaaaaagt gaatccctgt agtcgattct ttccatctat tttcattaaa atcttcagct
    17221 acaatcgttt ctcctactct atatataaaa ttttcatcag catatgcacg agcactttta
    17281 aaagattctt taccatctat actagtaatt gacagtactt ttgccttatc acatctacaa
    17341 gcgtttgatg tagcagacac tctttttgcg tcgctgggta ttaataattg taccattcta
    17401 aaattaaaac attttttata gcctataaat gctcctgttt caggacaata caatttaaaa
    17461 aaacgggtac tttcatctga tattactcca tttaaagtag catgttctaa atttgaattt
    17521 tctaaattag ctcctctaat atctgagtta gataagtcac aaaatctaaa acttgcacca
    17581 cttaagtctg cattttttaa tatagaattt tttaatgaac aatccgaaag ataagaatta
    17641 ttaaaaatag agttttctaa atctgctttt tctaaattta aattgataag gtcagaccac
    17701 gaaaagtcaa tattacttaa gcagtatcct cttaagtcca tattttctaa tcttaattct
    17761 tttaaatctg ccttttctcc agcattattg tttttaagcc ataaataatg tttctcaagt
    17821 gttttttcta attcttgttt agaaatagtt ttcattaaaa tcaccttcat aaatttattt
    17881 ttatatatgt agtagcaaaa
//
LOCUS       DAMJYC010000083        17773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJYC010000083 DAMJYC010000000
VERSION     DAMJYC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.085"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            128..685
                     /locus_tag="PC472_002942"
     CDS             128..685
                     /locus_tag="PC472_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728251.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            complement(1035..1397)
                     /locus_tag="PC472_002943"
     CDS             complement(1035..1397)
                     /locus_tag="PC472_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728252.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(1831..2640)
                     /locus_tag="PC472_002944"
     CDS             complement(1831..2640)
                     /locus_tag="PC472_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728253.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISIQVI"
     gene            2732..3355
                     /locus_tag="PC472_002945"
     CDS             2732..3355
                     /locus_tag="PC472_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HDF2728254.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            3352..4677
                     /locus_tag="PC472_002946"
     CDS             3352..4677
                     /locus_tag="PC472_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728255.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIKKQKKYHY"
     gene            4698..5237
                     /locus_tag="PC472_002947"
     CDS             4698..5237
                     /locus_tag="PC472_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728256.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            5256..6647
                     /locus_tag="PC472_002948"
     CDS             5256..6647
                     /locus_tag="PC472_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HDF2728257.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFADYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVETRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIMLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            6662..7753
                     /gene="ytvI"
                     /locus_tag="PC472_002949"
     CDS             6662..7753
                     /gene="ytvI"
                     /locus_tag="PC472_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2728258.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     misc_binding    8056..8269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8523..9557
                     /locus_tag="PC472_002950"
     CDS             8523..9557
                     /locus_tag="PC472_002950"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2728259.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            9598..10815
                     /gene="argJ"
                     /locus_tag="PC472_002951"
     CDS             9598..10815
                     /gene="argJ"
                     /locus_tag="PC472_002951"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2728260.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            10830..11690
                     /gene="argB"
                     /locus_tag="PC472_002952"
     CDS             10830..11690
                     /gene="argB"
                     /locus_tag="PC472_002952"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2728261.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            11720..12907
                     /locus_tag="PC472_002953"
     CDS             11720..12907
                     /locus_tag="PC472_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2728262.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            13016..14038
                     /gene="argF"
                     /locus_tag="PC472_002954"
     CDS             13016..14038
                     /gene="argF"
                     /locus_tag="PC472_002954"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2728263.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            14165..14782
                     /locus_tag="PC472_002955"
     CDS             14165..14782
                     /locus_tag="PC472_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2728264.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(15002..15700)
                     /locus_tag="PC472_002956"
     CDS             complement(15002..15700)
                     /locus_tag="PC472_002956"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2728265.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(15862..17079)
                     /locus_tag="PC472_002957"
     CDS             complement(15862..17079)
                     /locus_tag="PC472_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2728266.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(17270..17683)
                     /locus_tag="PC472_002958"
     CDS             complement(17270..17683)
                     /locus_tag="PC472_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728267.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         6575 a         2072 c         3007 g         6119 t
ORIGIN      
        1 aaattataat taaactgtca agattttata atagtataaa gaattttata aattacaata
       61 attgacgaaa ctaattagat tagctaaact aataaagtaa ttaattttaa atgaaagagg
      121 gaaaatcatg cgtgttagta gagaaatagt tatacaagct acatcagata tagttaataa
      181 agatgggctt aacaaagttt ctttaaaagt tattgcagaa aagctaggaa tacgtacacc
      241 atctttgtac aatcatatag aaagtctaga tgatttatta cttgaagtag ctcataaagg
      301 aatgagagag atgaatagtc aaatgataaa ggcggctatt ggaaattttg gagatacagc
      361 tataaagtgt ataagtatag catactttaa ttttataatt tctaatcctg gtgtttatga
      421 aacaattcag tgggctactt ggcatggaaa taatgaaaca ttagaaatct ttgatgaata
      481 taaatcctta ttagaaaaac ttattctttc ttgtaatcta aatacaaaga atacaggtga
      541 agtattaaac ttaataatga gtgtacttca tggttattcg actttgcaac ttgggaaatc
      601 acttttgagt aaagaagaag ctataaaagg tctagtcgat tctattgata ctgtgttatt
      661 aggtattcat aaaaaatatg attaaggagg taaataaatt attttaactt atttattgaa
      721 cacttcatta cgtgggatgt aaaataaaat tttatcattt ttatgaaagg aaaatagtat
      781 atttcaagaa tatttacttt ctgaattggt aaaccctatt tttactgttg tcgttgataa
      841 tgaaaaggta gctgtaaaat tccttgataa tcagacaaaa gcgacacagg tatcatagta
      901 taaattgata tttcataagg acagtaattg gaaaattatt ggataggtta tttgattata
      961 taaaaatgat tatataaaaa aagaaaagac cttagctaac aacacatatc aatttatacc
     1021 ttgaagctgt ggttttattt ttcaaattgt ttgcggattt ctctgtcttt acggattaca
     1081 gtttttgctg attttagcag ttcaactaaa gaacggattt catattcatc acaatctgag
     1141 aaaatatctt tggcttcctt ttcaaaaatt actttggaag aaagtacatt atcacataag
     1201 agagtatcga ctgatacaga aagtgcattg gcaatcgcaa tcaaggttgg caggctgact
     1261 ttcgtttttc ctgtttccac gttgctcata tgggatggtg tcatgttgat aatgtttgca
     1321 acggattctt gtgttatacc ttttttaatt cttgcaattt taattcgctg tccgattgct
     1381 ttataatcaa tttccatagt ttttgttctc ctttcattct tttaaatatt gtaactattt
     1441 acagagcata atataatact ctatataaat gaataattta tataatatat atttaagaaa
     1501 aaaatttgca ctacttttaa gacaactaca aaagaaatgg aggcataaag cctccatgtt
     1561 aagattttat aaaaagatag taaaaaactg attaaatggt taacgaaaat aaaaggctat
     1621 ttctgtatct gaaattcatg aggaaatgca gtttgcattg aggaaacaaa taatagcaat
     1681 gaacctgaat ttctacaaaa atttggaaat aagataccaa ctttagaaga taaaaaagga
     1741 cattttaagc aaattattat agacaatacg agatattaaa aaaggcgtgt atttctcatg
     1801 tgatttgaga tatacacgct ttttatattt tcaaataacc tgtatagaaa ttttttgaat
     1861 gtagttttcg gcgttatgtt ctgataaata atcaattaaa tcttcttcat aaatatttcc
     1921 aacaatatga tacccttgtt cttctaactg atttttcata ttttgataag ttttatataa
     1981 actgtcataa ctcccctggt gatacataac tgcataattt ccctttggtt taataaaaag
     2041 gcgctcatca tctactttat ttttaataag gctataataa tagctctctc taaaaattcc
     2101 ctgttttata tttttttcaa gaacaatttc tcctacatga aaattataat catgtaataa
     2161 acaatattgt aagtgatttc caataccttg taacattgtt ttttcatctg ttagtacagc
     2221 ttcaggaatt ttagttgcaa tcagatattc ttcctcacaa tttacaattt cgattttttc
     2281 tgtgtttaca ttgagatgta tttccattgt tgaaatgaca tttctcaaca tagaacacat
     2341 tttttcaatc ttcttcactt cttcttgaag atccattagc tttctgtgaa gtacattaag
     2401 aaaggtattc gtatctaaat cattgagata ttgttttatt tcttctaaag gtgtacctgc
     2461 tttttttagc acagaaataa tattaaattt ttcaaattgg tcaattgtat aataccgata
     2521 gccattttca tgggtaatac tcggttttaa taattcaatt tcatcataat ggtaaagtgt
     2581 atgttttttt accccacaca attctgcaaa ttcacttgtc ttatataatt ttttaatcat
     2641 ttttaaatct cctattgact ttagggttac cagatacttt aggataacat agaaagcttt
     2701 tatattcaat gaaatgtgag gaaaagatat tatgaaaatt attacaatta gccgagagtt
     2761 tggaagtggt ggacgagaac ttggaaaaca acttgctgat attttaaatt ttgactacta
     2821 cgataaagaa attatcactg ctatagctag aaagaaaaaa attgacgaaa actatgtttc
     2881 ctacatatta gaaaatcatg gttggagaaa cgttccttta acaatgtgta cttcttttac
     2941 taatattttg tctatgcaat ccatgaagac agatttgtta atagaacaat caaatgttat
     3001 caatcagatt gccagtacag gtaaggattg tgttattgtt ggtagaaatg cagatatatt
     3061 attaaagaag taccagccat ttaacgtttt tgtttgtgca aatatgaact ttaaaataca
     3121 acgctgtatt gaaaaaaagt taaacaatga acatatttca aaaaaagaat tagaaagaaa
     3181 gataagagag attgataaaa atcgagcaag atcaagagaa ttttttacga gctccttatg
     3241 gggaaatcaa acttcctatc atattatagt taatacatct gaatggaata taaaacatct
     3301 ttcttatgca attgctaact tttcagagta ttggtttagg aggtatgaac aatgatacag
     3361 ttgtcagata attttaatta taaacgctta ttaaaattta catttcctac aattattatg
     3421 ctggtaataa gttctattta tggggttgtt gatggatatt ttgtttcaaa ttttgtaggg
     3481 aaaactgctt ttactgctgt caattttatt atgccatttt tgctaatctt agggtgtgtt
     3541 ggctttcttt ttggaacagg tggtggagct ttaattgcta aaacaatggg tgaaggaaaa
     3601 aaagaagaag caaacgaaca gttttcgtta ctcattgtta cttctgctgg ttgtggtatt
     3661 attctagctg ttttaggtat agtaactatg ccatggattg cttctgctat gggagcagat
     3721 ggtcaattat tgacagatag tattctttat ggatgtgtgg taattatcgc tattcctgcc
     3781 tatattttgc agtgcgaatt tcagtgttta tttgcaacag cagaaaagcc aaaacttgga
     3841 ttatatgttg caattatggc tggaattaca aatattgttt tagacgcatt atttatcact
     3901 gtttttaaat ggggattggt aggtgcagca ctggcaactg caatcggtca atatgttggt
     3961 ggtatcattc cattacttta ttttagccga caaaacaaca gtttgcttaa atttaagaaa
     4021 cctaaatttg attgtgatgt tttgaaaaag actgttgcaa atggttcttc agaactaatg
     4081 actaatgttt ccacatctgt tgtgagtatg ctgtataatg ctcaattact aaaatatgct
     4141 ggcgaagatg gcgttgcatc ctatggtgtt cttctttatg tagcgttact atttcaagct
     4201 gtttttattg ggtattctgt tggtactgcc cctataatca gttatcatta tggagcaaaa
     4261 aattatgacg aattaaaaaa tttaagaaaa agaagtttga ttgtaattat ttgttttgct
     4321 gtatttatgt ttataatagg cgaattttta tcaaaaccga ttgcttttat ttttgtgggt
     4381 tacgataata atttaatgga aatgacatta agagcattta tgatttactc tttttccttt
     4441 ttattttctg gttttggtgt ttttggttct agtttcttta ctgcattaaa tgacggttta
     4501 acttctgcgc ttattgcttt tatgcgtatt ttagtatttc aagttgtatc agtacttata
     4561 ttccctatat tctggggagt aaatgggata tggttttcta ttgttggtgc tgaaatattg
     4621 gcaactatta tgacggcttt ctttataata aaaaaacaga aaaaatatca ttattaaatc
     4681 aatttggagg tatctatatg agcttaacaa tacaaaaagt aacttcatat tttaaagatt
     4741 tagaaaaatt tgaagaactt aataacgagg catttccaga tgaagaacgt atgtctatac
     4801 aagaaatgct taatttagtt caaagtaaaa aaatagaagt atctgcttta tatgataagc
     4861 aacaatttat tggtttttat gttttggtga ttgaaaatca tataggatat atattatttc
     4921 ttgcaatttc tccagaacaa cgctctaaag gatatggtgg aaaagcactg tcacttatga
     4981 aaaaacaata tcccaattgt caaattgttc ttgatatgga aactatagag gacagtgctc
     5041 ctaatttaca gcaaagaatt ttaaggaagc aattttatct tcagaatggg ttttatgaaa
     5101 cagaatatct catggagtat aagggactag aaatggaagt tttatgtaat cagaaaacct
     5161 ttcaaaaaaa tgaatttgaa acattgttga aaaatcttaa aattagagaa attccatttc
     5221 atttgtggaa aaagtaagaa agtgaggtat tagaaatgaa aagacgttta gctggggaat
     5281 gggaaccgat taaaggtgtc atgcttgcat ggcccccttt tcttccccat aagtatattg
     5341 tagatttaac gcaaaattat tctgtataca tgtgtgtacc aaataaagag gaaattgaaa
     5401 ttgcaaaata ttatttaagt aagtggggtg gaaatttaaa aaatttgatt ttctgcattg
     5461 ctccacaggg atttgataca ccatgggttc gtgattgggg tatgcaccct atttttgatg
     5521 aaaacggaaa actctttttg gctggtgcag aatataagat gtcaacacca tttgtatcca
     5581 taaataaacc agaaatcatg tttgattgcg aacataacct attaactact aactcttatg
     5641 aacaaaaaga agatcaatca caagagttga ttgcgaaaca gattggtttg ccatttatga
     5701 aattacctta tgcactaaca ggcggaaatc ttatgtcaga tggtttcaat aaattgatgt
     5761 ctatgcacat tttacagaca gaaaatcgtg cgaaaggaat ttctgacgaa gaattttttt
     5821 ctatggtggc aagagatact ggaatgaatg agtattatat attttctaat tttgctgatt
     5881 atggtttaca acatattgac tgttatttaa aaataattga tgaagaaaca ttacttgtga
     5941 gcagaacgcc aaaagaacac tatttgtatg aaagatatga aaaaatttta gaagaagtaa
     6001 aaaaggtaga aaccagatat ggacgtccat tcaaaatctt tcgtatagat attgttccgt
     6061 ttcaaaagga cagtgatgaa cttactgctt atgttaattc tgtgatttta aatagtcgtg
     6121 tctatgttcc aatgtatgga attgaacagg ataaaattgc aataaaacaa tggcagaacg
     6181 ctttgcctgg atatgaaatt aaaggatatg agtttgattt tgaaaaagag ccagatatta
     6241 taaaaaatcg tacaggatat gtgagaactg gctggggaaa tgaagatgtt attcattgca
     6301 ggacaagagc aatttgggat gagaatatgc tctatatttc cgtgaaaaga ttagaagaaa
     6361 tcgtttcaga gaatgatgct ttagaagtta ctattcaaat agttgattat agtaaagctg
     6421 gcttagatta tgaaaattgt agactgtttt atcgatacta tggttttcaa acatgggaaa
     6481 gtattatgtt agaagaaact acagaagcag aaatattttt tgccaatatg ataggaaagt
     6541 caggtgatat gattgaatat tttgtgcaag caaaaagttg ttctggaatg tgtgaaacaa
     6601 tgccccctgt tgctccaaaa ggcacatata aagttactat aaagtaggag gtgcatttta
     6661 tttgaatgaa caagatcata catcatacca aaataagcag agatttttaa taaatttctg
     6721 ttatttggta gtgatattag gcttactaat gttattaggg aaatatgcgt tttctgtatt
     6781 acttcctttt gtttttgcat ttttaatagc aagtttattg aataaaccag tcttatacat
     6841 aagtgaaaaa ttactaataa agcgaggtat tgtagcaacc atatctgttt ttttattctt
     6901 tttaattgca ggaataatag taagtgtaat aggtacatat ctaatatatg gaataaagga
     6961 aatttttcat ttcctaccaa ccatgtttga ggaattggta atacctttga ctgaaacagt
     7021 tataaaagaa gtcgaactat tttctggttc attcaatttt tcgtttattg aattaataga
     7081 agcaaatatg cctgtattga tgaatgaaat gagtgttttc atagtttctc tttcaaataa
     7141 tatcgtatct ggtattactg atattgtttc gtctgttcca ttactattta tgaaaacaat
     7201 tattacaatc gttgcttcta tttttatagc aattgattat ccaacgataa agagatttat
     7261 agtgctacag atacctaaaa ataaaagtta tcttttagca gaagcaaaag cgtttactat
     7321 tgataccatt attaaatgtg gtttttctta tcttttaatt tttgctatca ctttttctga
     7381 attatatgtc ggttttgcta ttattaatat ttcttatgct ggaataattg ctttattcat
     7441 tgctctttta gatatactac ctgttttagg aactggaagt attctaatac cttggtgtat
     7501 tatcagttta tttttagggg attattcaat ggcattagga attgcactac tatatattgt
     7561 gattactatt atcaggaata ttattgaacc aaaattagtt ggtaaacata tggagttaca
     7621 tcctgtattg gccttagcca gtatgctaac aggattacat ttctttggat ttattgggtt
     7681 gttcggtatt ccattgttaa ttgcgttttt gaaaaagtta aatgacaaag aaatcataca
     7741 tatattacat taggaaagaa tagttgtatg tatgtctctt ttatggagaa aattgtgttt
     7801 tttctaaaaa acgctggatg aaaaataatt ttgacaaagg tatagctttt gaatgacttg
     7861 attcaagagg gagaactttt ttaaaatata ttacagcagg aaattcatgg tgtcctatta
     7921 ctgtagatat ttataactca atttagatac aaaatgctgg aaattaatta tatagaaaat
     7981 atttccaaaa atttaaaaat tatattgaca aagttaaaaa aataaactat catataaaac
     8041 aataagaaaa taaattgaaa tcctgtgaaa aagagagtag cataaagcta gttgatagag
     8101 agctgggaat ggtggaagcc tagtaacgaa ttttattgtg aatagagctt tggaggagat
     8161 taccagaagt aatagtatgg taattggtga atctcgcctt aagagattag agtgggtaag
     8221 attatcttat caattagagt ggtaacgcgg gtgtatattc gtctcttggt ctttgaccaa
     8281 gaggcttttt taattttaaa tgtacatttt gtagatactt tttaatttga gttttaatga
     8341 ttattgtaat tttaccaata agagtggtac cgcggaaaat cgcctcttag tatttataat
     8401 gctaaggggt tttttgtttt aattagagta ttttaatcta cgtattatca aatgattatc
     8461 atatagtaaa gtggaaagat aggattttaa gactatttga atataaaaat ggagggaaca
     8521 aaatgaataa aataaaagtt ggtattattg gtgcaactgg atatgtcggg gcagagttga
     8581 ttagattatt aatgaatcat gataaagtcg aagtgacagc gataggttca aattcgtatg
     8641 ttggaaaaga tatagttgat atatatccaa gtataggata taaaaataac atgatatgta
     8701 tagaaaatga aaaagttata gatatgtgtg atgttgtatt tacagccctt ccacatggag
     8761 ttagtgaaaa gtttgtaata aaagcaatta aatcgaaaaa gaaagttata gatttaggtg
     8821 ctgactttag aataaaagat gaagaagtat atagtaaatg gtatggagta tcttttatag
     8881 ataaaatctt acataaaaaa gctgtatatg gattaagtga aatatacaaa gaagatataa
     8941 aagatgcaga tataattgca aatcctggat gttatccaac tagtatttct ttaccattga
     9001 tgccattact aagttctaaa ttgataaaaa ataataatat aataattgat tctaaatcag
     9061 gtcttacagg tgctggtaga gaattatcga ttagtagtca ctttacggag gttaatgaaa
     9121 atatcactgc atataaaata gggaaacaca gacatactcc agaaatagaa caaaatttaa
     9181 gtgaatcctg caaagaaaaa gtaagtgtag tttttacacc aaacttaatc ccagtaaata
     9241 ggggaatctt atcaactata tattgtacaa aagaagataa tatttctata aatgatatac
     9301 ataggaaatt aactgattat tatgaattta aagaatttat agaaattctt ccattagata
     9361 aggtagcttc tttaaagaat gttagattct caaataaatg tgtaatatca ttacatgaaa
     9421 atggagatac attgataatc tgttcagcta tagataatat gattaaggga gcagcaggtc
     9481 aagctataca aaacatgaat ataatatttg gtattgaaga aaatacaggt ttaaaaaata
     9541 ttgcaccatc attttagaaa taaaaaatac taaactcata taattaggag gataaatatg
     9601 gagattataa agggtggagt tacagtttca gaaggatttt ttgcttcagg gattcactgt
     9661 ggcttgagaa aaaataaaga aaaaagagat ttagctttgg tttattcaga tgttttatgt
     9721 gatgcagcag cagtagtaac tcaaaataaa gtaaagggca atcctgttta tgttacacag
     9781 gaacacatta aaaatggaaa agcacaagct atcatagtaa atagtgctaa tgctaatacc
     9841 tttaatggaa aagaaggact tataaatgct tataaaatgg ctaaattcac cagtgataaa
     9901 ttaaatatta aagaaagtga tgttttggtt gcatctacag gtgttatagg aaagccatta
     9961 aatatagatt taatagaaga aaatatggat gaattagtga ataatctaag taaacaaggt
    10021 catattggag caagagaagc tattatgaca actgatatta taaaaaaaga aatagcagta
    10081 gcaataatgt atggagataa aaaaataact attggaggta tggcaaaagg ttctggcatg
    10141 atacatccta atatggcaac aacgttagga tttataacta cagatgcaaa tattgatgga
    10201 gttttgctaa aagaagcttt aaaaattgct gttgacaaaa gttttaatag agttagtgta
    10261 gatggagata caagtactaa tgatatggta atgattctag caaatggaaa agcaaaaaat
    10321 gatagaataa acaaaaaaga tgaacattat caagtatttt tgagtgcact tacatatgtc
    10381 tgtatagaac ttgctaaact tgttgcaaaa gatggagaag gatgtacaaa actaattgaa
    10441 tgctatataa atggagcatt aagtgaagaa catgcagtaa aattagcaaa aacagttata
    10501 tcttcttcat tagtaaagac agctgtattt ggagctgacg caaattgggg tagaatactg
    10561 tgtgctttag gttatgcagg tgaagaaata gatatggaaa aagttgatat tatttttgag
    10621 agtatgaaag gatatattga agtatgtaaa aatggaaatg gattagattt taatgaggaa
    10681 aaagcaaaaa aaatccttga agatgacgaa ataagtatat tagtagattt aaatatgggc
    10741 aatgccagag gaaatgcatg gggatgtgat ttaagctatg attatgtaag aataaatgga
    10801 agttatagaa attaggagga tatactttaa tgattaatat agaaaaagca aatacactta
    10861 tagaagcttt accatatata gaaaaacatc agggaaagac tattgtagtt aagtatggtg
    10921 gaagtgcaat gaaaaaagat ggtctaaaag aatctgttat ggaagacctt gtgttaatga
    10981 gttatgttgg aatcaatata gttttagtac atggtggagg ggcagaaatc aataagatgc
    11041 tagcaaaagt ggatatagaa agtaaatttg taaatggtct tagatataca gatgaagaga
    11101 ccatggaaat tgtaaaaatg gtacttgctg gaaaggttaa taaagactta gtaaataaaa
    11161 tacatacaaa aggtggaaaa gcagtagggc tttgtggaat agataataat atgattttat
    11221 gtgacccata taaaaattat gaacttggat ttgtaggaga aatcaaaaaa gtaaatgttg
    11281 agcttataga atcttgtcta aagtcaggtt atatttcagt aattgcaact ataggtgttg
    11341 gagatgatgg agagacttat aatataaatg gtgacacagc agcttctgct atagctaaag
    11401 agttaaatgc agataaattg atacttctta cagatgtccc tggtttgtta agagagccag
    11461 atgaagaaaa atcattaata acagaagtta tattagagga tgttgataaa ttatttgaag
    11521 aaggtataat tactggaggt atgataccta aaatagaggg atgcgtagat gccttaaata
    11581 acggagtaaa tagggtacat attttggatg gaagagttcc acattctata ataacagagc
    11641 tatttactga tagtggaata ggtacattaa taagaaaaga aactgaataa aattataagt
    11701 ttaagagggg agaataagaa tgaataacga caatacaatt tcaaaatgga atgaatattt
    11761 tatagataca tataaccaac ccaattttgt catagattat ggtgaaggaa gttgtttttt
    11821 tgatactaat ggcaataaat atatagattt tacaagtgga tatggagtat cttctcttgg
    11881 gtatagtaat agtaatttaa agaatgctct aaaagagcaa gtagataaat tattacatac
    11941 ttcaaattta tattttaatg agccagtgtt gtattcagga gagaaaataa ttaattcaag
    12001 tggaatggct aaagtatatt tttgtaattc tggaacagaa gcaaatgaaa cagcattcaa
    12061 aatagcaaga aagtatagta gtgacaaata tggtaatggt agaggaacta tcataagttt
    12121 aaaagactct tttcatggta gaacaatgat gtctttaatg gcaactggta tggacaaata
    12181 ccataagtat ttttatcctc ttcccgaagg ttttaaatat atagaaagaa ataatattga
    12241 agatttaaaa aataatcttg attccacagt ttgtgcaata attcttgaag cgattcaagg
    12301 cgaaggtgga gttaatgttc ttgaaaaaga ttatgttttg gaaatagtaa aaatatgtca
    12361 agaaaaggat attgtagtga tttttgatga agtccaatgc ggtataggaa gaactggtaa
    12421 actttttggc tatgaatatt ttgatgtaaa accagatata gttaccgtag caaaagggtt
    12481 gggagcagga ataccagtag gaggagtatt ggtaaataaa aaactttcaa aagtactagg
    12541 aaaaggagac caaggtacaa cttttggagg taatttatta gctatggtgg cagcttcagt
    12601 tgtattagat gagatatcta aagatggctt ttataatgaa gtattagaaa aaggaaatta
    12661 tataagaaaa tctattgaaa gttttaataa caaagttata ttaaagacaa aaggtattgg
    12721 tctaatgata ggaattgaaa ctaatattga atctagtatt atagaagaga aagctagaaa
    12781 aaaaggattg cttattttga ctgctggaaa aaatgtattg agatttttac caccattaac
    12841 aatcagttat aaggaaatag atgaggctct tgaaatccta aaagatatat tactagaaat
    12901 taactaaata ttcaaaaaat agttgattaa agataaaaat tagaagtaaa taaaaaagta
    12961 aagtgctaac aagtttaatt gaatttaaaa agtttataag aaggggcgaa ttaatatgaa
    13021 taatataagt ttaaaaggta agagtttttt gacattaaag gattttacaa aagaagagat
    13081 tagatattta ttaactttat caagttattt aaaaactaag aaaaaagttg gtataaaagg
    13141 cgatttgcta caaggaaaaa atatagcttt attatttgag aaaacatcaa caagaactag
    13201 atgttcattt gaagtagcag ctcatgatga aggagcacat gtaacttatc taggtccaaa
    13261 tgattgtcat atgggtaaaa aagagtctgt atcagataca gcaaaagttc taggaagatt
    13321 ctatgatgga atagaattta gaggctttaa acaagaaaca gttgaaagtc tagcaaaata
    13381 ttctggtgta cctgtttgga atggacttac agatgaatat catccaacac aaatactggc
    13441 agatttttta acagtaattg aaaatgtaga taaagatttg aataaagtta aatttgtata
    13501 tgttggtgat gctagaaata atatggggaa ctcactgatg ataggatgtg ctaaaatggg
    13561 aatacatttc gtagcactag cacctaagga actttggcca aatgaagaat tggttaagga
    13621 aatgaaggaa atagccaaga aatccaaagg agatataatg ttgactgaaa atattgatga
    13681 tgttaaaggt gcagatgtta tatatacaga tgtatgggtt tctatgggag aagaggagca
    13741 atatgaagca agaataaatc aacttaaaga ttatcaagta aatatggata tgataaagaa
    13801 aactgaaaat gaaaatgtta tatttttaca ctgtcttcca gcatttcatg atttagaaac
    13861 taaggttgct caggagattt atgaaaagtt cggattatca gaattagagg ttacaaacga
    13921 ggtatttaat tcaaaatatt caaaggtatt tgaagaagct gaaaatagaa tgcatacaat
    13981 aaaagctgtt atggtttcta ctatgactga tgtaaatatg aacaataatt taatatagat
    14041 atgttttaat atagacttta ctttaatgaa aatttataaa atattttaaa tatattattc
    14101 aaaaactctc attatatgtt aaaataactt atgtatcaac aaattaagag ggaggatttg
    14161 agatatgaaa aaaatcatat tagctttaac aactttttta ttagcaatat ctttaacagc
    14221 atgttcaagt gcaaaaccag aagatacaat tgatagtttt tttaacagtg cgaaaaagtt
    14281 tgactttgaa ggcatgaata aggtaatgga aaataatgac gaaaaatata aggatatatt
    14341 aaaggaatta gatacaaaag acccaaatgc ccaatatgtt ttagattatc taaaacagaa
    14401 tgcatctaaa atcacatata caataaaaga cagcgaggtt aaaggtgata aggctacaat
    14461 caaagtagaa tgtaaattta tagattcaac accattactt caagaaatag tagctgaggc
    14521 atttacaaag atgattggta tgagtttttc aggacaagac ttaactgatg agaaaacaac
    14581 tgaaatgtta gtatcaatta tgaaagaaaa acaaaaaagc gttaaagaga cttatgttac
    14641 aaaaaatgta gagtttgaat gttctaaaaa ggataataaa tggataataa gttcagtaaa
    14701 tgatgcagta gctgatgtat tgttatctaa tcttgttaca gcaggacaag aattttctaa
    14761 ctctatgaat acacttgaat aatattgttt aggtgtagta aagtaataaa tatatgatta
    14821 tttttataaa taaacatata aatatttatg aaggatacca tttttggtat cctttttatt
    14881 aatataatga aatacatggt taatttctat attgtcaaga aactatgata tatgttataa
    14941 gtctatacta tttaaaataa acttataaca tatatttttt ataatatagt gtatgaaaaa
    15001 tttatttaac taattttaga tatttattga caactgattc agccaagcat tttgatgaat
    15061 caacaatatt tattgaagga tatgattttt ctaaaactac atttaaatca gtacaagcaa
    15121 ttactatatt ttctatagat tcatttttaa catcttcaat aagtttattc caaatatcaa
    15181 tattgtgtgg attttcttta tccattttta tattttgaat aagattgttt aattttattt
    15241 gccattcatc tttaaatata aattcatgtc cattatgtat tataccattc tggtaaattg
    15301 tagactcaaa agttgaacta gtcgaaaaaa gagttacttt ttgtgagata gtaggtaatt
    15361 ttttaactgt ttcttctaca atatttaata ttggtatatt aatagactct tttaattctt
    15421 taaagtatat atgtgctgaa ttgcatggca ttgcaataaa atttacacca gttgattcaa
    15481 gcttttgtaa tccatcaatt atagtttctt tcataaggtc atgatttatt ggacggtcaa
    15541 tataaaatgg ggtaggtaat gaatatatca tcatctttgg aaattcatca tcatattttg
    15601 ctccatattg agattgacac tcatcaataa ttaaatcaac aaaaggggct gttgaccttg
    15661 gacccattcc agccaaaata ccaattaatt tttcattcat aaaaattatc tcctttatat
    15721 ataaatatat ttatgtataa aataaaaatc tataaaagca taaaattata ttttataata
    15781 gattatattg tacacttata taatataaat ataaagtgat tttttagatt atatatttta
    15841 atatactttc ttatataaga attattttaa atcaagatta tctagtattt ttaaaaatat
    15901 ttttacagca gtatccaagt catctacaga gacactttca ttaggattat gacttacgcc
    15961 cccagcgcat cttataaata ccataccaat atcagttata ttatccattt cctgagcatc
    16021 atggccagca ccactataaa tataaaaagg attaagattt aaatctataa aacttttttc
    16081 tatgattttt gttattttat tagaacatgg tacaggaaca ttttcaaaag caagttcact
    16141 tgtataatta agttttctat tttcacatat atgtgatatt tcattaaata tcttttctac
    16201 agaattatca agaatttctt gtgaacaaga gcgtacatcg agtgtaaatt cagctctttc
    16261 aggtaccaca tttgatgaag atggatagaa attcatcttt cctacagtag caaccaaatc
    16321 agtagtcgtt ttagctactt tttcgatagc taatacacat tcacaggcac aacatccagc
    16381 atcaagtctc atattcattg gaagtgtacc agcatgtcca gacttaccat ttactgatat
    16441 cttaaaactt ttaaatcctg taatacttga tacaattcca gcaggaagag attcattttc
    16501 taaaacagga ccctgctcaa tatgaaattc taaatatgca tctacatctt ttctaggatg
    16561 caaatttgtt aagttttttg tattaaatcc aaactttgtt acagcttctt caagtgtaat
    16621 tccattttca tcaacagatt taagaagttc ctctttaaaa gtaccagcaa aaactttact
    16681 tcccattaaa gaagtttcaa atctagttcc ttcctcttca gcgaaagcaa ttagcttaat
    16741 attgaaagga taagacctat tattgtgttt tgcttcttca agaccaacta gaggcactat
    16801 tatacctaac ataccatcaa atatacctcc acactttaca ctatcttgat gagatgcaac
    16861 tactaatgtg ggagcatttg gaaagcttga tttatatgta cctataacgt ttccaatgtc
    16921 atcaatttca atatcaagat ttaaaccttc catccatgaa ctaattaaat caatagattt
    16981 tttatgttct tgagtcaaga taagtcttgt aagaccattt gttgttgaac ttatttttcc
    17041 taatgtttgt agataatcca tacatttttt tcctaacata tttacctcct aaaaatataa
    17101 tttttattta agctaagatt agcttttata cttataattt ttgaactttc aaataatttt
    17161 gtatgtatag taaaatacta ttttagtatt aataatgata taaaaacata aagtctgtag
    17221 acgaagagca gaagttttaa tttaaaaata tgaaatatga tttttattat tatagtattt
    17281 ttctataata atatctcttt ccagttagag gataattttc aagtgaaaat actttatggt
    17341 atccaaattt ctcataaaat ttaggtgctt gaaaactgaa tgtatctaaa aaaacatatt
    17401 tacaacctcg ttctttagca attttttcag ccttatataa aagttgactt ccagtacctt
    17461 tatatctaag cttctcacta acccataagt attttataga aagccaatta ccatgagtaa
    17521 cacctataaa acctgcaaca attttacctg ctttatctcg aatatatatt cctaaatcca
    17581 ctgggttttt atcttcaagt ctagcaagat tatattttaa taatccttga aaaatagtat
    17641 cttcatcttg tttttctatt ttttctgtaa tttctaattc catatttgaa ctttctcctt
    17701 gtataatctt ttgtatattg gtatttttta ataaaatcag tagaaagaat atagtttaac
    17761 attattctaa tag
//
LOCUS       DAMJYC010000084        17545 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJYC010000084 DAMJYC010000000
VERSION     DAMJYC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.086"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            319..777
                     /locus_tag="PC472_002959"
     CDS             319..777
                     /locus_tag="PC472_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2728268.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMAIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            838..1113
                     /locus_tag="PC472_002960"
     CDS             838..1113
                     /locus_tag="PC472_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2728269.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1172..2446
                     /locus_tag="PC472_002961"
     CDS             1172..2446
                     /locus_tag="PC472_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2728270.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2576..3628
                     /locus_tag="PC472_002962"
     CDS             2576..3628
                     /locus_tag="PC472_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2728271.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DNHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDTNPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3628..4659
                     /locus_tag="PC472_002963"
     CDS             3628..4659
                     /locus_tag="PC472_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2728272.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGTTVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4751..5563
                     /locus_tag="PC472_002964"
     CDS             4751..5563
                     /locus_tag="PC472_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2728273.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5566..6486
                     /locus_tag="PC472_002965"
     CDS             5566..6486
                     /locus_tag="PC472_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2728274.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6661..7107
                     /gene="rpiB"
                     /locus_tag="PC472_002966"
     CDS             6661..7107
                     /gene="rpiB"
                     /locus_tag="PC472_002966"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2728275.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7137..7802
                     /locus_tag="PC472_002967"
     CDS             7137..7802
                     /locus_tag="PC472_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2728276.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7912..9414
                     /locus_tag="PC472_002968"
     CDS             7912..9414
                     /locus_tag="PC472_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2728277.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9726..11315)
                     /locus_tag="PC472_002969"
     CDS             complement(9726..11315)
                     /locus_tag="PC472_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2728278.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNI
                     NYYYREFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKF
                     LENVISLFSKVKIELTLILTLILLTLRFHAFTSNKIFYNTNLIIYNFIIISIVYIIYE
                     NIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFM
                     YIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVI
                     GDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDML
                     KNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQS
                     IVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDM
                     FIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENA
                     HMEIIIDADLFKVMLTFRRVN"
     gene            complement(11284..12000)
                     /locus_tag="PC472_002970"
     CDS             complement(11284..12000)
                     /locus_tag="PC472_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728279.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12540..13340)
                     /locus_tag="PC472_002971"
     CDS             complement(12540..13340)
                     /locus_tag="PC472_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2728280.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13654..14385)
                     /locus_tag="PC472_002972"
     CDS             complement(13654..14385)
                     /locus_tag="PC472_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728281.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14372..15067)
                     /locus_tag="PC472_002973"
     CDS             complement(14372..15067)
                     /locus_tag="PC472_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2728282.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAKPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15069..15860)
                     /locus_tag="PC472_002974"
     CDS             complement(15069..15860)
                     /locus_tag="PC472_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728283.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15870..16682)
                     /gene="modA"
                     /locus_tag="PC472_002975"
     CDS             complement(15870..16682)
                     /gene="modA"
                     /locus_tag="PC472_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2728284.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(17317..17517)
                     /locus_tag="PC472_002976"
     CDS             complement(17317..17517)
                     /locus_tag="PC472_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2728285.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT         6683 a         2145 c         2767 g         5950 t
ORIGIN      
        1 ataataaata attttataaa atagttgtta aacataaaaa accatttgta aaaaacaaaa
       61 aaatcatttc tctaacaaaa aaaaccacaa aaaatataaa aaagtatggc tagttaagtg
      121 attaacaagt aaaaattaaa ataaaaatca aaaaaaattt atcaatgaaa gttagctaaa
      181 atcctggaag agagcattat ttttgggaaa acattttctt ttgtttcaca aaaaaagcca
      241 ctttttggca cacaacatgc tttatataaa gtcaagaaaa actaaggtat aagacaaagg
      301 caattaggag gtgtaaatat ggagtttata aattttgtaa gtgataaagt tatagacctt
      361 aaaatggatt gtaaaacgaa agaagatttc tttaaagaaa tacataataa agtttttgat
      421 ttaggctttg taaaagaaga atttggagaa aaaatattag caagagaaaa tgtatttcca
      481 acaggtttaa atttaggtga ttatggagta gcaataccac atacagatgc agaatatatt
      541 aaagagcaat ttatatcagt ctgtactttt aatgaaccag tagtatttag ttctatggaa
      601 gaccaagatg aaaaagtacc tgtaaatttg gctttcgtat taggacttaa tcaaccacac
      661 agtcaattat ctgtattaac agaattaatg gcaattatgc aaaataaaga gttagttgat
      721 aagttgatga gttctactga taaagaagaa gttttaaaga cactaaagtc tttataaaaa
      781 taaaaaagta ctaaaaataa aaaatataaa aataaaaatt attaggagga aataaaaatg
      841 aaaaaaatat tagtagcatg tggagcagga atagcaacat caacagtagt ttgtgacaag
      901 gtagagagat tagttaagga aaataatata caagcagaag ttatacaatg caaaatagct
      961 gaatgtgcaa caaagcaaga aggagcagat ttaatagtat caacaactat actaccaaca
     1021 acttataata taccagcaat aaaagcaaca gcatatataa ctggagtaaa tacagcagca
     1081 ttagataaaa aaatattaga tgctttaaaa taatcctatc atagatacat tgaaataata
     1141 ttttataaaa cagaaaaagg aggatttata aatggataaa ttattagcag tagtacaata
     1201 cattttaaat atgggaccga cagtaatact accaataaca attgtagtac taggaatgat
     1261 atttagaatt aagtttacaa aagctttaaa gtcaggtctt acaattggta taggatttgt
     1321 gggtataaac ttagttgtaa acctattaac atcaacacta gggccagcgg ctcaaggaat
     1381 ggttgaaaga ttcggactta atttaacagt aatagatgtt ggttggccaa caacagcatc
     1441 agcaacatgg gcttcaccag ttgcaccaat attaatacca gtttgtttat tagtaaactt
     1501 actattatta tacttcaata aaacaaagat attaaacgtt gatatatgga attattggca
     1561 ctttatagta gcaggtgcag ttggatatat agtaacagga agttatgttt gggctatatt
     1621 ctgtgcaata ctaatggagt tattagtact gtttattgca gaaaagacag caaaaaatgt
     1681 tcaggagttt tatgatttag aaggtatagc attaccaact ggttcaacag cttcatttgc
     1741 tccacttgga tatttagtag gtaaatttgt tgaaaaaata ccaggaataa ataaaataca
     1801 agcagaccca gactctatac aaaaaagatt tggtatattt ggagaaccaa tgatgatggg
     1861 gattatacta ggtataatac ttggagcact tgcgggatat gatgtagcag gaatattcaa
     1921 tgtaggtata tcaatgggtg cagtaatgtt acttatgcct agaatggtta gaatattaat
     1981 ggaaggttta attccaattt cagaatcaat aagagatttc ttacaagcaa aatatggaga
     2041 cagagaactt tatataggtc tggatgcagc agtagcaact ggacatccag cagttatatc
     2101 aacagctctt atacttgttc ctataacttt gttcttagca gttatactac caggaaacaa
     2161 agttttacca tttggagact tagcgacaat accattttat atggcattta tagtgtgttt
     2221 tagaaaaggt aatatagttc aatcagttat aacaggaaca atagtaatag ctatatcact
     2281 ttatatggct acaaactttg cagatgttca tacacaatta ttacaacaag caaatatgat
     2341 gccagaagga acaacaatgg ttacaagtat agatacaggt ggaaacttgc taaaatgggg
     2401 aatattgaaa ttatctcaat tagtttcttc atttggaata ttctagtatg tagttaatag
     2461 agattactct tttttagtaa agtatgcgaa aatttaacat ttagcagcta ctaaaaaaga
     2521 gtaatatttt ttataaattt ttaaaaatac aaaatacaag aggaggagat ttatcgtgaa
     2581 agcagcagta ttacatggca caaatgatat gagatttgaa gatatagaaa taaaaccatg
     2641 tgaaagtgat gaagtaaaaa taaaagttat ggcagcagga atatgtgggt cagatcctcc
     2701 aagagtatta aaacattgga aatatccagt tccagctata ccaggacatg agttttctgg
     2761 agtaattgct gaagttggta aggatgtaaa aaatgtaaaa gttggagata gagtagttgc
     2821 aattcctttt ataccttgta atgaatgtga atattgtaaa agaggattat tttcattgtg
     2881 tgataaccat ggaatgttag gagctaaaag ttttggagct tttgcagagt atgtaaatat
     2941 aaaagctaca aatgtacttc caataggaga tatgaacttt gaagatgctg caatgataga
     3001 gccactagca gttgctatgc atggagtact taatataggt gttcaagttg gagacactgt
     3061 agctgtaatg ggtagtggaa caatggggca gttggtaata caaggactta aaatagcagg
     3121 ggcaggaaca attatagcag ttgatatatc tgataataaa ttaagagaat caaaggaatt
     3181 aggggctgat ataataatta atgctaaaga tactaaccca gtagaaaaaa taaaagaact
     3241 tactggtgga aaaggtgttg acatagctct tgaatgtgca ggttcaaaaa taactcaaga
     3301 acaatgttta cttataacta aaaagaaaag taagatagga tttttaggga tagcatattc
     3361 agatataact ttatcagaag aagcatttga aaatatattt agaaaagaac tagaattaaa
     3421 aggtttttgg aactcttatt ctgcaccatt tccaggacaa gaatggacta agggaatcaa
     3481 tctagttaat gaaggaaaga taaaattaaa agaaatggta tctcatagat tctcacttga
     3541 agatacttac aaagcatttg aaatgataag agatagaaaa gaagaattta acaaaatatt
     3601 aattttacca caaggggttg aaaaataatg aaagcagtag ttaaaactaa accaggctat
     3661 gataatgtag aagttttaga agtagaagaa ccaaaagcta caggagacaa ggttaaaata
     3721 aaagtagaat atagtggtat atgtggttca gatatacatt catttaaagg tgaatatgca
     3781 aatataaaag ccccagtaac attaggtcat gaattttctg gaattgttgt agaagtagga
     3841 gaagatgtta aaaatataaa agtaggagat agagttacat cagagactac ttttgcaact
     3901 tgtgagaaat gtatatattg tgaaactaaa gattataact tatgcccaac tagaaaaggt
     3961 attggtactc aagtaaatgg aagttttgct gagtatgtat tatcaagaga agaaagcata
     4021 catgtacttc ctgaaaatgt atctttatta tcagcagccc ttacagagcc actagcttgt
     4081 tgtgttcatg cagctcttga aaagactact atagaaagtt ctgatacagt tttaataata
     4141 ggaccaggac caattggttt attattagct caagttgtta aatctcaagg tactacagtt
     4201 ataatgtctg gtgttacaaa agataaggaa agattagaaa tagctaaaaa tcttggagta
     4261 gatagaactg taaatgtaat ggaagaaagt ttggcagatg tagtgaatga aatgactgat
     4321 ggaaatggag taaacaaagg atttgattgt tctgggttta tgccagcagt taacgaagcc
     4381 ttaagactgc ttagaaaaaa aggtactttc gtacaagttg gaatctttgc taaaaaatta
     4441 aatgaaatgg accaagaagc aataatccaa agagagttac aatatatagg aagcaggtct
     4501 caaaagccaa gttcttggat tacagcatta gatttaatgg caaatgggaa agtaaataca
     4561 gaagctttag ttactagaac tgttggttta gacgaattta aagaaggtat tacagcttta
     4621 atgaacgggg aagaaataaa agtagcagta aaatcttaaa aaaacatttg tattatttta
     4681 aaaaaaattg taaaatctac tgtaggataa aaaaatgtaa ggtcttaaaa aataacagga
     4741 ggaagaaagt atgagcactc ttagagatca ttctaataat attagaaaaa acataataaa
     4801 aatggttgca gaagctaaaa gcggacatcc aggtggttca ttatcaatag ctgatatgtt
     4861 gacagtatta tattttgata agatgaacgt atgtgcagaa aatccaaaaa tggctgatag
     4921 agatagattt gttctatcaa aaggtcatgc agcaccagct ttatatgcta cattagcaga
     4981 aaaaggattt ttcccagaag aagaattaat aacactaaga aaatttggtt ctaagttaca
     5041 aggtcaccca gacatgaaaa aagtagcagg aatcgatatg tctactggtt ctcttggtca
     5101 agggctatct gcagcaaatg gtatggcact tgcaggaaaa ttagataaca aagactatac
     5161 agtatatact atattaggtg atggagaaat ccaagaaggt caaatatggg aagctgcaat
     5221 gacagcatca cattacaaat tagataattt aattgcattt gttgacttaa atggattaca
     5281 aatagatggt tctaatgaag aagtaatgaa tgtaaatcca gtagatgaca aattcaaatc
     5341 ttttggatgg aatgtaatag taataaatgg acattctttt gaagaaatag gaaatgcagt
     5401 tgatgaagct aaaaaagtta caggaaaacc aactgtaata atagctaaaa cagtaaaagg
     5461 taaaggtgtt tcatttatgg aaaataatgc tgcatggcat ggaacagctc caaatgcaga
     5521 acaagcagag caagcattga aagatttaga aggaggtcta taggaatggg aatagcaact
     5581 agagaagctt acggacaagt gttaaaagaa cttgcagaaa ataaagatat agtagtatta
     5641 gatgcagatt taggaaaagc tacaaaaagt atatcattta aagaagttgc cccagataga
     5701 ttttttgaca tggggatagc agaaggtgac atgattggta cagcagcagg tcttgctact
     5761 tgtggaaaaa taccttttgc gagtactttt gcaatatttg cagcaggaag aggctatgag
     5821 caaataagaa actctgtagc atatccaaat ttaaatgtaa aaatagcagc tactcatgca
     5881 ggtgtaacag taggtgaaga tggtggtagc catcaagcta tagaggatat atctctaatg
     5941 agaggtatac caaatatggt tgtattaaat ccagcagatg ctttagaagc aagacaagct
     6001 attcttgcat ctattgatta taatggacct gtttatataa gattaggtag agctgcaact
     6061 cctgatgtaa acagtgaaaa ttataaattt gaaataggaa aaggaactgt tttaagagaa
     6121 ggttctgaca taacagtaat agctacagga ataatggtag caaaagcctt agaagcagca
     6181 gaagaacttg ctaaagaagg tataaatgta gaagttgtta atatatctac aataaaacca
     6241 ttagatgaag cattaataaa agaaactgct aagaaaactg gaaaagtagt tacagcagag
     6301 gagcacagta taataggtgg tttaggttca gctgtatgtg aagctttagc agaaactaaa
     6361 gatgtagtag ttagaagaat tggtgttaaa gatgtatttg gtcaatcagg tacaccagct
     6421 gatttattaa aacactatgg tttaactaca gaagatatag taaaaaatat aaaagagtta
     6481 ttatagatat aaatatatct aaataacaaa ttgatttgac ataagtcaat taaagctgtc
     6541 ttaaaaatag aaagaaattt ctacaatggg acagctttat ttttacaata ctttattttt
     6601 atgataatta taaaatagtc tgaaagtaat caaaaaaatt ataaaaagag gtgctattta
     6661 atgaaaatat ctattggatg tgaccatggt ggttatgaat taaaggaata tataaaggaa
     6721 aatttaataa ataaaggtat agaagttgaa gatgtgggta caaattcaaa agaaagtgta
     6781 gattttccaa tatatgcaaa aaaggtaagt caaaatgttc aaagtaaaaa atctgattta
     6841 ggcatacttt gttgtggaac aggaataggt atgtctattg cagcaaataa gtttaaagga
     6901 atcagagcag ctgttgtttc tgactgtttt tcagctaagg caactagaga acacaataat
     6961 acaaatgtac tttgtttagg agaaagagtt attgggaaag gattagcaat gagaattgtt
     7021 gaagaatggt taaattcaag ttataatgga gaaagacatg caagaagatt gcaaatgatt
     7081 gatgatatag aaaaagaaaa tttataaata tataatttat aacaaggggg agcgttatgt
     7141 ctattatttg tgcttcaata atgtgtgcag accaaatgaa gttaaaagag gaattagaag
     7201 ctcttgaaga agcaggtgta aaattacttc attgtgatgt tatggatgga atatttgtta
     7261 aaaacttggc tatgggacca gagttattaa aatcaataag tgaaaataca acaatacctt
     7321 tagacataca cttagccaca gaaactccag ataaatatat tgatatgatg tcttatataa
     7381 agccaaaata tatttctttt catgtagaat caagtacaaa tgtaaaagct gatatacaaa
     7441 agttaagaaa ttatggtata gggccagtgt tagcaataag ccctcaaaca agtgtggata
     7501 aaatagaaga atatataagt ttagttgagg gtatcttagt aatgacagta aatccagggt
     7561 ttgcaggtca aaagtttaat ctttctgtat tggataagtt ggataaactt acagagatac
     7621 taaaagatta tgataatcct ccatttatag aggttgatgg aaatataaat aaggatacta
     7681 ttaaacttat gaatggtaaa aaagtagata tatatgttgt tggtacttca gctttattta
     7741 atgataagcc tccaatttca tataaagata aaatagaaga actaaaagaa agtataaagt
     7801 aaccatgtaa taaaattata atatgtttct gtttaatagt aaaaatttag gtggaaacaa
     7861 gaaattttat aatatttaat aaaaattttg gttgagtaag gagaattttt tatgggagac
     7921 ctatataaac tacaaaatgg tactgatatt agaggaatag cgtatgaaaa tgattctaaa
     7981 gaagttaacc taactgtaga agaagtaaaa aaaatagcta aagcgtttca tatttggtta
     8041 aaagaaaaga ctaagaaaga taaagtaact gttgccatag gaactgattc taggataaca
     8101 ggaagccaat ttagaagtac tgttattgaa actttaacta atgatgattg taatgtaatt
     8161 gattgtgaga ttgccacaac tccagctatg tttatgacaa ctattatgga tggatacaat
     8221 tctgatgggg cgattatgat tacagcaagt cacttacctt attactataa tggtttaaag
     8281 tttttcacag aaagtggagg tttagaaaaa actgatataa aagagatgtt ggatatagct
     8341 gttaaaaatg atagtgtaga ctatgaggaa gtaaataaaa aaggtgaagt tatagtaaaa
     8401 aatttaatag aagattattc taatttatta atagataaaa ttagaaaagg tgtaaattca
     8461 agtaaaaatt atgaaaagcc tttttctggg cttaagatat tggttgatgc aggaaatggg
     8521 gctggtggat tctttgctga aaaagtttta cacatactgg gagcagatac gacaggtagt
     8581 cagtttttga acccagatgg tatgttccca aatcatatac ctaatcccga aaacaaagaa
     8641 gcaatggaaa gcatatgtaa agctgtgtta gataataaga gcgacctagg aataatcttt
     8701 gatacagatg tagacagagc tgctattgta ggtaaaaatg gcaaaccaat aaataaaaat
     8761 gctcttatag ctgttatatc atcaatagta ttagaagaac atccaaaaac agctatagta
     8821 acagattcga ttacttctga aggacttgct aaatttataa atgaattaca aggaagacat
     8881 catagattta aaagaggata taaaaatgta ataaatgaag ctataagatt aaatagtgaa
     8941 ggtgaagagt gccatttagc tatagaaacg tctggacatg ctgctttaaa agaaaattat
     9001 ttcttagatg atggggcata tttaatagct aaaattttaa taaaagtagc aaagctatcc
     9061 ttagaaggaa agactataga agaattgata gaaaaccttg aagaagctca agaagagaaa
     9121 gaagttagaa taggcattaa taaagaagat tttagaccat atgcagagga tatattaaaa
     9181 gatttagatt ctcatgtcaa gagtattgat ggatggtctg tagcaccaaa taattttgaa
     9241 ggcataagag ttaattgtga tgaatcaaat ggagatggat ggtttttact tagaatatca
     9301 ctacatgaac cattattagc tttaaatatt gaatcaaata aaattggcgg agcagaacaa
     9361 atttattcta agttaaaact tttcttagaa aaatatgatt taaaaggtct ttagggtaaa
     9421 tagaaagaat atatttatat tgaagaataa atgtaaattt gtacctaaga ttacaaatat
     9481 tacaaaattg taatcttagg taacagagat gtaactgatt atggaaacac tatgtgatat
     9541 tatagataca gagagatgaa atatgtgatg aattatagag aaatataatt taaaatgtat
     9601 taatatcaca ccaaaaaggt ataatcaaaa aatatgtaaa tataaacccc aacatttaga
     9661 aaaagacctc taaggaggtt tttttcttgt ttaaataact taaaatatat ttttatatca
     9721 atatatcaat taactcttct aaaagtaagc attaccttaa ataaatcagc atctataatt
     9781 atttccatgt gtgcattttc taattcaatt atattttgag caattgataa accaagacca
     9841 gagccatctg tttttctaga tatatcaccc cttctaaatc tttctattaa ctcttctggc
     9901 tttagattta gtttatcatt tgaaatattt ttaaacgtta atattatatt ttcttccttt
     9961 ataaacatat ctatatagac cctagtgtta ggcatagagt attttaatat attgcaaatt
    10021 agattctcaa aaactctcca agttttttta ccatctatca tggtaaaaac agcatattca
    10081 ggtgtttcta ttataaaatc taaactgcta ttttgaattt tgtcttcaaa ttctacaata
    10141 ctttgaatta aaagttctct tatatcggtt tttgttatat taaattctac atctccacta
    10201 cttagcttag atgcttcaaa tatgtcatct attaataatt ttaatctttg tgatttttta
    10261 tctaaaatat ttatatagtc atttatagtc tctctgtcaa agtcattatt ttttaacata
    10321 tcaacatagc ttaatataga tgtaagtgga gtttttaagt catgagatac gtttgtgata
    10381 agctctgcct tcatcctttc attttttata ttagcatcta cagcagtttc aagaccgcct
    10441 gttatattat ttatactttt ggctaaagta gatatttcat catctcctat aacttctaat
    10501 ttatacttta aattgccatt ttctatattt tttatatttt ttgatatgtt atttatgtaa
    10561 ttaaacttaa cagataaaaa gttgtatata taaaaatgaa aaactgtgtt tacaagtgta
    10621 aataaaatta ctatagctat tgcctcagca taatatatat acataaagaa tatcaaaaca
    10681 agatattgga taatagcttg tacaactata gatattaaaa atactatagc taatttttta
    10741 tttatagttt ttttattata catactaagt aagtttgaaa agaatacata tgttatagac
    10801 atatctttta gattatagtt attatattta atattttcat atattatata gactatagat
    10861 ataattataa aattgtagat tattaaatta gtgttgtaaa atattttatt acttgtaaat
    10921 gcatgaaacc ttaaggttaa tagtatcaaa gttaatataa gtgtaagttc tatttttacc
    10981 ttagaaaaaa ggcttatgac attctctaag aatttgactt tagtttgttt atttttttta
    11041 tacaatgtaa aacatagtat agtcaataaa aaacttgata atataatacc tatgcagaca
    11101 aactctaatt ctacttttga caaatgtaca ggagtaccga aaaaataaaa ataagggaga
    11161 gcaaactctc tataataata attaatatta gaaaagttat tattcaaatc ataaactgct
    11221 tcaaataata tagaaactaa tagaaacagt aataataata aaataaattt tgctttagaa
    11281 tcattaataa ttaagttttt caattttgta accaatgccc cacaccacct ttaaatattt
    11341 tggttctttt ggattaattt caatcttttc tctaattctt ctaatatgaa cagcaatagt
    11401 tctctcacaa ctataagcat cctcattcca gacattttca taaatctcat gagaagagaa
    11461 aattttacct tttcgtgaca taagaagtgt aagtatttta tattcaagtg gtgtcatttt
    11521 aatttcttca ccatctaaag aaactatttt tgtatctgta tttagctcta ggccaccact
    11581 ttttagaata taattagtat catttgagtt atttgtatta ctaaaatcac ctaaatttat
    11641 ataccttcta agttgagatt ttactcgagc agtaagttct agtggattaa atggtttagt
    11701 tatatagtca tcagcaccca tattaagacc tataattttg tccatatctt ctgattttgc
    11761 agataagaga attattggta tattcctttt ttctcttatt ttaagtgtag ttttaagccc
    11821 atctaaattg ggcatcatta catccattat tattaactta atatcatttt cgattagtat
    11881 gtccaatgct tcaagtccat catatgcctt taaaacatta aagttatcag gtctcaaata
    11941 gaactctata gcatcaacta tctctctatc atcatctaca accaatatat tttgattcaa
    12001 cttattaacc cccttgaaat tttattttta accaattttt attttatacc aaaaagaggt
    12061 attagaaaac agtaagccca ttcattatga aataaaaact agtactttta atatattgtc
    12121 aatgcatata gtaaatttaa aagtatagac gcccatatta atctgaaggt aaaagtttat
    12181 gaaaatatct agggtaatct atcacacttc ttatgttatt ttggtttatt aattttttag
    12241 gtttaaacat atattaacaa atataaataa caaatgagtt tcataattat taagaattta
    12301 taacaaatat ttattttaat gtagtttaat ataaacaggt agtttaatgt aggtatgtat
    12361 aaagcattta ataaggctta taggttttaa tgatgatatc tttatatatt ttaagagatg
    12421 atgaaattag atatgggtaa tagttgtatg agaaaaagta gtatagcaca aaaagtgcta
    12481 tatcacaaaa tctcaaataa tttccttttt ataacttaaa ttaaaagaat aaatatttct
    12541 tataaattag gtgtatgaag tatagcagga acatctttat tatttaataa cttatcaaat
    12601 gatttatggt cagacttata gccaatacct tctctatatt ctaattcatc aacaattttg
    12661 tattgttgac aggcattttt aaccttttca tagtcttcag tagaatgagc tttataaact
    12721 atcttattat taaaagttat tttatcagca aatttaggtt ttacaaaata tttagcatct
    12781 tttattttac cagcattata taaattttgg ataacagaac cattttttaa atgttgtaaa
    12841 tggtcatcta acttataagt atgtgctata tgagttacat ttttaaattt ttcttcaaat
    12901 gtaagagcaa ttttttccat gtaattgaag tcagtattac catttgtatt tgtttctggt
    12961 aataatatta tattttccct tattacgcca aggctattaa gagaagctaa aaattctcta
    13021 tttctatatt ctgatttttg tatatttgtt aggttttcat attttttata tttatcaaag
    13081 acggatatac cacatccagt agatactaaa accacaaata catgattttt tcctctttct
    13141 tttatagcat taacaatagc actcccagcc cacaacactt catcatcttg atgttgaaca
    13201 tagaagacaa cataatcttt aaatttttta tgactataat tcacatagct actaaaagcg
    13261 gttaaaatca aacataaaac tagaataata gatagaattt tatatttatt tatagtaaat
    13321 tttttattct taaatgtcat ttaatcatct cctgaaaagt tgtcttagtt atattatcaa
    13381 acaaagtact aaaaattatt tcttaaatgc tgaaaaatat agagtagatt aatggaataa
    13441 aattttttta ttagtttaaa ataaagaaaa catttatgta taagactaat caaaatatta
    13501 atgattgata ggaattgaga tttaaaggga ttgtacattt attcgggatt cattttaata
    13561 gaattttact ttagtactta gaataaaagt aataactttt taaatttaaa gagtacacat
    13621 atatatttgt gtactccttg atgatatgat tgcttattta ctacaaaaac agagtctttt
    13681 taaacctaaa atatttgatg caccagctat cgtagttcca taatagtaaa ctggtttgtt
    13741 aagtgataga aaatttataa ttgagttatt acataatgta cttcctgtgc aaatcactaa
    13801 atcagcccaa gatattacat cttcataatc tcgtatacca tcttcaatct taacattata
    13861 ttgaatagta tccacaaatt caggatttaa atctaatact cttgtttcga aaaagtcctt
    13921 aatattatca attatggctg gttgatatcc tataattgct acttttggat taccaaattc
    13981 catttttata aactcaggga atttttttgc acatacctct ggctcgttat ttttacaatg
    14041 aatggttcta tccgtcttgc ctaagtattt cataactgca tttaatgatg ctataaaaag
    14101 acttctatca tgaggattgt catgtaaatc taatgataat atgtctttta aagaaccttc
    14161 aaatgtagat ggagtagaag taaatgcttg tcctttagct cctttaaagt cagcttcaag
    14221 cattatttct ttaccaacta taattggaaa atctttacgc ttagtatttc caattgcttc
    14281 ttttgaagat agagatttgg acacaatact tatcatatcc atatttaaat tatatttatc
    14341 gacttgtact aatagataat ctctcaaaat atcaaatata ttcatagaaa aaagattctc
    14401 ctttattttt tatttcttca tttaaatcaa aaatatgatt tatttttcca ttgttcatta
    14461 ttcctatctt atgagcaaaa aacattgctt cagaaatatc atgagtaaca tgaatcactg
    14521 ttggtttgta ttttatgtag acatccttca aaattttgct tatatgtttt tttgtaatat
    14581 aatccaatga actcataggt tcatctaaaa gtaatatatc aggtttagct attaaggcac
    14641 gtgcaaaagc aactcgttgc ttttcgcctc cacttaaatt tttaggatac ctatttttta
    14701 tatcttctat ctgcattaat ttagacaagt ggttagcctt aagaatctgt tcatctatag
    14761 gtatttttct taatttaagt ggaaacctta tattatcttc aacattcata tttggaaata
    14821 attcaaaatt ttgatataca aatcctatat ttctattttc tggaggtaag tttgatatta
    14881 aattttcttt gtatgataca tttcctttag cagatggata tcttcctgct aatgtttcta
    14941 ggaatagagt tttacctgac ccactagctc ctaataaaac aaagtattca tgattattaa
    15001 tatttaaact attttcttgt aatttaaaat taggtaaatc aagagataaa ttttttattt
    15061 ttaacatatt aattcttatc aacctctcta ttaaaaaata tctcaaaaag catcaagcat
    15121 gtaagtgaga taaatatcaa aattacagct attccgattg caatgtctat atcaccagta
    15181 gacatattta aatatataga tgtaggaata atttcagttt tcatacgtgt agaacctgct
    15241 atcatagcta ctgcaccaaa ttcaccaagt gctcttgacc aagtcattac agttgcagat
    15301 atgattccag tttttagatt tggaagaatt ataaatctaa atgcttccca actattacat
    15361 ccaagggtac gtgcaacaaa tatcatcttt tcattacttt catttagtga agtgtgaagt
    15421 attttaatag taagtggaag atttacaaat acttgtgcta aaactatacc ttgctttgta
    15481 aatataaaat caagcttaaa aatcttataa atggaatctc caatccccat tcttccaaac
    15541 aaaagtaaaa gagcaattcc tgatactaag tgtggaagag acatagggag atttattatc
    15601 tgtataatca gttttttaaa tggcaattta gtaatatgta aaaaatatgc tactggaata
    15661 gctaataaaa ggcatactat agtagatatt aaagatgttt taatactaag tgttattgca
    15721 tactgtactt ccactgattt taagcatata ggaacataac ttatcccttt aatgattaac
    15781 ataagaagtg gtgatacaat aaacaaaaag actatagcaa aaaatccagt aagtagagta
    15841 gtaaaaaatt tatttttcaa gtttaatcac tacttaacag gttcaaaacc atatttttta
    15901 aatatctgtt tagcttcgtc tgactctata taatttataa atttattagc taaatctttt
    15961 ttatcactat agtttacgat tcctgctgat ataagttggt ctatattttt atcttgggca
    16021 atttctataa cttctacatc ttcattacca aagaccgaat cttttgtggc tattgtagca
    16081 tcagctttac ttgatgttaa ctgtacaatc atttcattta cagttgcagc tgtagagaca
    16141 acattatcat ttatagattt taaattattt ttttcaagta ttttttgtgt agttttacct
    16201 atagcattag actctttgtc tcccaaaata agtttaactc catctttatt taagtcttcc
    16261 aatttttgaa tattagctgg atttccttta ggtacaacta ttgcaggtgt atgatgtgca
    16321 accgttttat gttcacctac aagttctttt tcaaaggcct ttgaataaat tggttcagaa
    16381 ccaacaataa atacgtctcc tttgtgactt gtctcaattt gagctattag ctgtgcagaa
    16441 cctgcatagc tatattcaac tttgacatta ttttctttct caaatttttt tgctatttca
    16501 tccattggtt tctttaaacc tgcccctgaa tagacaaaaa gagtctcttt ttcttttgaa
    16561 tcatcagctg ttgtcacttt agtattagat tcatcattag ctttgttttc tttagaatta
    16621 catgcagtaa tacttaatga aataatcata gctaaagcta gtactgaaag ttttttaaac
    16681 ataagatatt cccccttagt aaattagtgt aaattctttt ttagtatatc atactaagaa
    16741 aacgatgtct aagtttttgt tatgttttat aatgttttgt tatgttttgt tatgtatagt
    16801 taagaaaaaa tctatttata atgtcatttc cataggtagt tattttattt ttaattttta
    16861 ttaatacttt aaagtatttt aaatttaaaa acttaaatca aattaactta ttgtagattt
    16921 tttacttttt aactaatttt atatttatta tatttcagtg aaaaaataga acattgaaac
    16981 atatagatat gttttaattg ttttttatta ataaattact aaaagtgtta tatcagaatt
    17041 gaatacaaaa gaaagagtat gtatttatga gatagttgaa aattttgatg tgccacaact
    17101 aaaattatta tattactata tattctaatt aaaagagtac ttgtaggttt aagtacatac
    17161 tttataaata tatttttgtc agatttattt tataaaaagt atttagacta agtattattt
    17221 ttaagtattt ttttgttgaa gttatttttt ccttttttac aaaatgcgta aataaaaaaa
    17281 agactcttaa aagagtcttt ccgaaaaatc tatatattat aatatagtta tattttctgc
    17341 ttgaggacct ctagcgcctt taactatatc aaagcttact ttttgacctt cttctaatga
    17401 tttaaatcct gaagtttgta tagctgagaa atgagcaaat acgtcatctc cactttccat
    17461 tgatataaat ccaaatcctt tttcattatt aaaccatttt actattccat tattcattaa
    17521 aaatactcct attaacctat atcaa
//
LOCUS       DAMJYC010000085        17353 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJYC010000085 DAMJYC010000000
VERSION     DAMJYC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.087"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            110..1027
                     /locus_tag="PC472_002977"
     CDS             110..1027
                     /locus_tag="PC472_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2728286.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1105..1842
                     /gene="cas6"
                     /locus_tag="PC472_002978"
     CDS             1105..1842
                     /gene="cas6"
                     /locus_tag="PC472_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2728287.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1848..3725
                     /gene="cas8a1"
                     /locus_tag="PC472_002979"
     CDS             1848..3725
                     /gene="cas8a1"
                     /locus_tag="PC472_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2728288.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIDKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKVQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHITNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3727..4641
                     /gene="cas7i"
                     /locus_tag="PC472_002980"
     CDS             3727..4641
                     /gene="cas7i"
                     /locus_tag="PC472_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2728289.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4643..5344
                     /gene="cas5b"
                     /locus_tag="PC472_002981"
     CDS             4643..5344
                     /gene="cas5b"
                     /locus_tag="PC472_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2728290.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5355..7676
                     /locus_tag="PC472_002982"
     CDS             5355..7676
                     /locus_tag="PC472_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2728291.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEIVECNSNMN
                     IGEYTEKHLVKEFGSLREVQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSNEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QNGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            7691..8242
                     /gene="cas4"
                     /locus_tag="PC472_002983"
     CDS             7691..8242
                     /gene="cas4"
                     /locus_tag="PC472_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2728292.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8245..9225
                     /gene="cas1b"
                     /locus_tag="PC472_002984"
     CDS             8245..9225
                     /gene="cas1b"
                     /locus_tag="PC472_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2728293.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9228..9494
                     /gene="cas2"
                     /locus_tag="PC472_002985"
     CDS             9228..9494
                     /gene="cas2"
                     /locus_tag="PC472_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2728294.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9678..10035
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9678..9706
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            10225..10653
                     /locus_tag="PC472_002986"
     CDS             10225..10653
                     /locus_tag="PC472_002986"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2728295.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKRFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(11107..11886)
                     /locus_tag="PC472_002987"
     CDS             complement(11107..11886)
                     /locus_tag="PC472_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728296.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            12210..12755
                     /locus_tag="PC472_002988"
     CDS             12210..12755
                     /locus_tag="PC472_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2728297.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            12787..13923
                     /locus_tag="PC472_002989"
     CDS             12787..13923
                     /locus_tag="PC472_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2728298.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            13927..15318
                     /locus_tag="PC472_002990"
     CDS             13927..15318
                     /locus_tag="PC472_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2728299.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            15394..16269
                     /gene="dpsA"
                     /locus_tag="PC472_002991"
     CDS             15394..16269
                     /gene="dpsA"
                     /locus_tag="PC472_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2728300.1"
                     /translation="MSNQYDITVIGGDLRQMYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     STKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            16272..16862
                     /locus_tag="PC472_002992"
     CDS             16272..16862
                     /locus_tag="PC472_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2728301.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(17069..17269)
                     /locus_tag="PC472_002993"
     CDS             complement(17069..17269)
                     /locus_tag="PC472_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728302.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         7139 a         1670 c         2831 g         5713 t
ORIGIN      
        1 aaaatataaa gaggttatat gtacatatgt gtagaaataa ataataaaaa tatcaattaa
       61 tgttattagg ggggggttac tcattagtaa attagctaat acacttagaa tgatgatatt
      121 aattcaaagc agaggcaaaa tgaaatgcaa agaattatca gaagagttgg aagtaagtga
      181 aaggcaaata aaaagttaca agatgtattt agagcaagct ggtatattta taaattcaac
      241 acctgggatt tatggtggtt atgaaataga taaatgtaat agcatctcat taattaaatt
      301 attagatagt gaagtttcaa ttctagatat gataaattca cagttagagt ataataatga
      361 tatttacaaa aatgaattta ataatattgt agaaaaaata aaagcagtat taaatacagg
      421 agaaaaaagc gatacatata tggattattt tacagttcaa gcacaacgta attgtgatta
      481 tgagtctgaa aaaaataagt gtaatgaaat aattagagca tataccacaa aacacaaatt
      541 ttggatagaa tattattcat taaattcagg aaatagtgaa agaattgttc atccatatgg
      601 tttatttaat tataaatcag atacttatat ggtagctttt tgtgaaaaaa gatttaagtt
      661 tatagacttt aaattatgta ggattaaaga ttataaggtt ttagaagaaa aatataatgt
      721 agataaaagt tttagttggg atgaatatag taaaaatagt ataggaattt ataagggaga
      781 agaaattaat gttgttataa aaattagtca tccattttca acaattataa aggagaaagt
      841 ttgggttaat aatcaacaaa tcatagaata tgatgataaa tctataatgt ttaaagctaa
      901 aatgagaggt tatgaggaga taaaaagttg gattttaagt atgggagctt atgttgaggt
      961 tgtagaacca gatagactta gaaatgatat attgttagag attgaaaaaa tgaaaaaaat
     1021 ttactgattt tttcaaataa tttatacttg gaccaatata cttcacggtc tagtgttaat
     1081 atattaattg agagaggtga tattatgaaa atgaaaattg aatttagtac aggttgtata
     1141 ccaatatctt ataattcgct ctttatgagt attataaaag aagcaataag aaaatctaat
     1201 gaagattact ataaaaacat gtactattat aaagaaaaga ataataaaaa aactaaaaat
     1261 tttacatttt cggtttatat aaaaaagtat agtattgaag gtgacaattt tattatagaa
     1321 gataaagtaa tattaaatat aagtactcca gatttagaat tgggttttca tatatacaat
     1381 gggttaatga cttctaaaaa atgtttatat aaagactacg aacttacaag aattagaatc
     1441 gatttatcaa gagaaaagaa ggtaacagaa gaaagggtac tatttaatgc attgtctcca
     1501 atctgtgtaa agtctaaaga aggaaaattc ttggagataa ctgatgatag atacatagaa
     1561 gaactaaact atataactaa tgaagtggtt aaaaattata gggggaatgg attaaaaaga
     1621 aagttagaat ttgaaaatat aggtctcaaa aaggtagtag ttaaagagag tttaagagaa
     1681 tttaaaaaaa taacaggtaa agagtatcaa tatattaatg gttacaaagg taaatttata
     1741 ttgaaaggtg atattgatga tttaaatctt atctacaatc taggaattgg ttttagacgt
     1801 gctcagggat ttggagatgt agatatttta gaatggaggt gagataaatg aagttaagag
     1861 tttatagagg agattggttt tttaatatgg gtatagttgg atttttaaat ataataaaga
     1921 aagcagataa acaagctgaa atatttatta tggaagatta tatcgaattt gatagcttat
     1981 ttcttgaaaa ctttcatgag tattatttta attactttat ggatgaatat gatgtatcaa
     2041 aaagaattaa aaaaaatatt gattacagta taaattttat taaatctaag cctgatagaa
     2101 tcaaagatgg aattaagaaa attaaagata gtgtgaaaca acaaaatgat aagattaaaa
     2161 aatttgatga agaaaaattt aacatgataa aagagaagct agatagtatg tctaagataa
     2221 aaagttatga tgaatttgat agtttagaag ctttagtaga tgagactata gatatattta
     2281 aaataaaatc aataaatgac aggcttacag caaatcttta taaatatgta gttcaagata
     2341 attattttgg acaagttagt ttttttaatg ttgctaaggc gaaattagat ttagatggat
     2401 taaaacaggt aatgtttaat gattatttaa gacaaattat atattttgga gagttagaag
     2461 atttattaga agagaatgac tatgataagt tgaaaaacta tttaaatgat agattaaata
     2521 gtatagataa ggatataaat gaaaaaaaag ttagtaagtc tagtataaat actatagaaa
     2581 aaattatgaa ggaaataaat aaaaacttta taaagaaaaa taaaagcata gaagatataa
     2641 aagagtatat ggatagttta gaagtatgtg aaatgtgtgg tttatataag ggaatattag
     2701 atgaatactc agaaagtaat tttgctccac ttggagtaag caccaataat gctaggaata
     2761 tgttttggaa tcaggcgtat gtaccatcaa tatgtgatat ttgtaagcta atattgttct
     2821 gtactccagc aggggctact tatacaagaa agaattattt aattaatgaa gaaaatgagt
     2881 tttatttatt tgttaacatg gatacttgta taaatgagct ttttgaaaga aataatagtt
     2941 taaaagttca aaaaagtgag tcttcagatt ccaaagatga aaatcctttt aatcagctta
     3001 taaaaagtat agtagaagaa aatacattaa aaagtgaatg gcaacttcgt aatatacttt
     3061 ttgtggaatt taaagcaagt atagattcta aaaaatgtaa aataaattat tttaatattc
     3121 caacttattt agcaaagttt tttacagaca agtatgcaaa taaaaaaata caaagtatct
     3181 atgattacaa gcttaaatca aatgtactag atttactctt gaaaaatagg gacttgaagc
     3241 atataacgaa taacatttta agaaataaag ttaaaaatga tatggaaagc aacaacaaat
     3301 caaatatatc aggaatagat tgttttagag tagtccaatt aagagtatta ataaatagct
     3361 acaaaaaggg ggtatataaa atggatagta aaaatttgga gaagaatgat gaaaagctga
     3421 aaataattta ttatttaggg tgtgatatac atgactattt tgttaataaa aattctaaaa
     3481 ataaaataga tggagtaagc tataaacttt taaattcagt taaagtcgga aataaaagtg
     3541 attttatgga tacaattata agagttttta tgtcagctga aaaacagatt ccagcattta
     3601 ttttagatat agaaattgaa aaagatttag actttgaatc tataggtcat gcttttatat
     3661 ctggattaat ttctggaaaa tatgaaggaa aagataaact accaaacgaa aaggaggaaa
     3721 aataagatga aaagagggtt aacatttaca gtaatagcac aagctcaaag tttaaactat
     3781 ggtgaaggga taggtaatat atcagaatta aaaaaattaa gtagagatga tggaaatatg
     3841 tatacatttg catctcgcca gtgtttaaga tatgacatag tacgattagc agcagaattg
     3901 tttaattgga atttacaggt tgtcgataaa gaaaaaggta ctattcaatt taaagataat
     3961 ataagtataa gagaatcaga agaaatggat ttatttggat atatgaaaac aaataaaaaa
     4021 gatgagaaag ataaaaaagg aggttctttg actagagaag ctactgtaag attaagtaat
     4081 gcgatttctc ttgagccata tagaagtgat atggattttt taaataataa aggatttgca
     4141 gatagaatag gagaacaccc taatcttgct aacatagagc aacatttaag ttactataca
     4201 tatacagtga ctatagattt atctaaaata ggcaaggatg gagagataga acttgataat
     4261 aaagagaaat gtagaagagt agtagaattt ttagaaataa taaaagtatt gaatagaaat
     4321 attagaggta gacaagaaaa tttatctcct ctatttgtag taggaggagt atatgaaata
     4381 gcaaatccat ttttcttggg tagaattaaa ttaaaaggag ataaaaatgg atttaaaata
     4441 aataagcaag ctatagaaga agtaattcaa ggtacttttt taggaaaaga tttaaaagaa
     4501 tttacctatg ttggaatggt agatggggta tttataaata aagaagaatt taagggattg
     4561 tttgaagata attttttaag tgtagataaa ttttttggac aactagttaa agaagtaaag
     4621 gaatattatg gggtgaatta atatgaaggt tttgaaatta gatttatttc aagaaactgc
     4681 atgctacaaa aaaccatttg ctatgaagat tacagagaca tatccactgc ctccatattc
     4741 tacaataaat ggattaattc acaaaatatt aaatgctaca gaatatatac catttaatat
     4801 aagtgtacag ggaacatatg aaagtatatt taataattac cagacaacat atttttataa
     4861 aaaagatagt attacatcta tgccaatgaa tagtcatatg ctattgaatg taaatttgat
     4921 aattcatata ggtggggatt ttgatttatt ggaagaaata tatgatagtt tattaaatta
     4981 tgacgagcat ttatcattag gtagaaaaga agatttagta agaattaatg atattagatt
     5041 tgtagaagtc aataaatttg aagtagatga ggatatacaa ttagatgatt atgaaaatta
     5101 taagttatca gaatgtaata taagaatgcc tatatatata ccaaaaacaa gtttacaaga
     5161 tacagatata gatggtataa gttacagact caataattac tatgataata atgctgtgga
     5221 agataaaaaa agggtttgga ataaggtaga tagttattat gttgaggatg gaaatactat
     5281 atcaattgga gatatattat tagataatga aggagattta ttatatttta ataatataaa
     5341 ttaggaggga gattatgtta tatgctaaat ctaatccagt ggaaacactt agagaacata
     5401 ctgatgaact tttaaaacaa atgaatgtat taagagaaag ttatggaaaa aatataaata
     5461 gcttagattt tctagaagag gagatatttt ggcaattact tgactttgtt atagaatttc
     5521 atgatattgg aaaagcattt tcaccgtttc aagagttaat aaaaagcaga atggatacta
     5581 aaataaatga acctaaaata gtaactcatt tagaaaataa tgtaggccat aattatcttt
     5641 ctcctgcatt tattgattat agttatatag acaggaaaaa aaataaagaa cttagagcag
     5701 tattaaatca agttattgta tatcatcatg aaagagatat ctttatagat aaagatttta
     5761 aaaatttaat acaaaaaata ttagatgaag atttaataaa taaagtttat gaactacagc
     5821 atgaatttaa agttagatat ccaataaaaa cagaaaaatt gagtaaagta tatctacaaa
     5881 gtgtagaaaa aagaatagat aaaaatcaca agtactataa tttatatatt atgttaaaag
     5941 gaatactaca tagattagac cacagtgctt ctgcccatga gattgtagag tgcaatagta
     6001 atatgaatat aggcgaatac acagaaaaac atttagtaaa agagtttggt agtttaagag
     6061 aagttcagtc atttgctaaa tcgaatagaa acaagaatat aatactaatt gcatctacag
     6121 ggatgggaaa aacagaaact gctttgattt ggatagataa ggataaagca ttttttacac
     6181 taccattaag agtaagtata aatgcgttat ttgatagggc taaaaatata attggagtag
     6241 gagaagcaaa tgatacattc ttaggtctgt tacattcaac agcaatagat tatttagaag
     6301 aaagtaatca ggaaaattca aatgagattg tagatttagc aaagttatta tcttgtaaac
     6361 tgacattttc cacaatagac caaatattta aatttccttt tttgtataga ggctatgaaa
     6421 aagtgtactc aacattagca tattcaaaag tagtaataga tgagatacaa gcatattcac
     6481 cagaaatagc agctgtattg gtaaaaggta tagaaatgat acataagata ggtggcagat
     6541 ttatgataat gacagctact atgccaacca tatatataga tgaattaaaa aaaagaggtg
     6601 taatgaatag taacttagct gacttgacat gtaacacaga aaaaataaga cattgtgtgt
     6661 ctattgtaga aaattcaata gatgaaaact taggtaaaat aatacaaaat ggaatgaata
     6721 aaaaagttct tgtaatagta aatacagtta aaagtgctgt agaaaaatat gagttaatag
     6781 aagaaatagt taagccaaaa ggaatagatg tcaacttaaa tcttttacat tctatgtata
     6841 tacaagaaga tagagcaaaa ttagaaaaat atataaaaga atttgcagat agtgatagta
     6901 atggtatttg gattactaca caattagtgg aagcatctct tgatattgat tttgatgagc
     6961 ttcatacaga aaattcaaca cttgacagtc tatttcaacg atttggtaga tgttatagaa
     7021 gtcgtgagta tgaagaaaat agcccaaata tatttatata tacagaaaaa gctacaggaa
     7081 taggtagtat ttatgataag gatattgtag aaaaaggact tgagctactg aaaactttta
     7141 taaatggaaa agaatgtgtg aagatgaaag aaaaatataa ggtagaaatg gttaagatac
     7201 tatactctaa agaaagtcta gaaggtacag cttttgaaaa gagatttacc agtgctttaa
     7261 atattttaga tactataacg ccatatagtt taggttctaa agatgcacaa aatatactta
     7321 gaaatattga aggatataca gtaattccag aggatatata taataaaatt gaagaaactt
     7381 taatcaaaga ttatgaagag ttgggacaag agcttataaa tgcatatagt aatgatgata
     7441 gacaattgat aaataaaata aagtctaaga gaaaaaaagc aagaagagag ataattaaaa
     7501 aaactgtaaa tttaccaata tataaagcta aacaaaatgt aattgacata caggtgaaag
     7561 gtcttgaaga tttaaaaata cttttatata aatatgatat atacgaaaat aagcatacaa
     7621 aacagatatt tggtaaaggt gtgcttataa gtaatgaaat agaccaattt atttgatttt
     7681 tatattaaca atgtggctaa aatttactaa agaaggtgat tttgtgccaa tagatttaga
     7741 agtattaaaa gttcaaggtg taaaaataaa ttattattat gtatgtaaaa gaaaattatg
     7801 gctattttct aaaggaataa ccatggaaca aaatagtagt agagtattgt ctgggaagat
     7861 agtacatgaa gattcatata aaagaatgaa aaataaagaa atattgattg atgatatctt
     7921 gaagctagat ataataaagg acgagtatgt aaaagaagta aaaataagtt caaagatgaa
     7981 agaagcagat gaaatgcagt tatactatta cttatactat ttaaagaata gtatgggtat
     8041 agagaaaaaa ggaactataa attatgttaa ggaaaagaaa caagaagaat tgatattgac
     8101 agaagaaaaa gaaagaatta ttgaaaacac acttataaat ataaatgaaa taaatatgca
     8161 gttatctcct cccaaaattg agaaaaaaca ttattgtaca aaatgtgctt attatgaatt
     8221 ttgttttgtg aaggaggagt aacaatgtat aaagactatt atattataag taatggggat
     8281 ataaaaagga aagaaaatac gatttactat tacaatttag aaggtgagaa aaaaatatta
     8341 ccagtagaac aagttgatga tattcatata tatggagaag tagatttaaa cacaaaactt
     8401 ataaattaca taagtaaata tggggtgatg cttcatttct acaattacta tggatattat
     8461 tcaggtacat attatccaag gaagaaaaat gtatcaggat tcagtttagt atgtcaatca
     8521 gcctgctatt tagataaaaa tgaaagatta tacttagcaa aatcctttgt ggaaagtgca
     8581 tcataccata ttttaagaaa tatgagatat tacaaagttg atgaaagttt aattgatact
     8641 ataaaggatg aactaaatga tttgaatgat gtcaaagaaa tatcagaact tatgggtaaa
     8701 gaaggcagaa taagaaaaac atactataaa tcatttaatc aaatattaaa agatgatttt
     8761 gaattttata aaagagaaaa aagacctccc aaagacccag taaatgctct tatgtctttt
     8821 ggaaatagca taatgtatac gaatgtttta agtgagatat ataagacaca acttgaccct
     8881 acaataagct ttttacatga accatcaaca aagagatttt ctttaagttt agatattgca
     8941 gaaattttta agccaattat aattgacaca ataattttta gcctgattaa taataaaaga
     9001 attaataaga atgattttga atatcaagac aaaatttgtt atttaagtga aaaaggaaag
     9061 aagaaatttt tgcaagaatt tgaaaataag atgcagacaa ctataaaaca tagaaatcta
     9121 aatagaaaag tttcatatag aatgtttata aggttagagt gttataaatt aattaaacat
     9181 tttataaatg atgaaaggta caaagtttta aaggtgtggt ggtaagtatg tttgttattg
     9241 ttacttatga tattgttgaa gcaaggtcgt taaatagaat tagaaggata cttagaaaat
     9301 atttgacttg gacgcaaaat tctgtttttg aaggcaatat tactgaagga aagttacata
     9361 aatgtatttc tgaaatagaa aatattattg ataatagcga ggattcaatc tatgtttatg
     9421 agataaaaaa tcctaattca attaaaaaga aatgttatgg gattgataag tattctgatg
     9481 aaatgtttat ataggtttgc agtgagcgat atttatgcta aaataggtgt taacagttgg
     9541 aatataaggg attgaaggtg tatgataact gttatcaatt gcactactgc tcgctcactg
     9601 caaattttga tgtttttatt gaattataat tgcttgattg aagtgttttc aatgtattca
     9661 aatataccta ttttggggtt ttatattaac taagtggtat gtaaattttt tcatttttat
     9721 ttttttagtt gcattttcaa ctggttttat attaactaag tggtatgtaa attatttttt
     9781 agctttttct ctatatgtac caatatcacg ttttatatta actaagtggt atgtaaatta
     9841 gaaatacaat tcggaacttg tttctgttga atttgtttta tattaactaa gtggtatgta
     9901 aataggttta aaaacctctg ttcttccacc ataataccca gttttatatt aactaagtgg
     9961 tatgtaaata ggataaagaa aagactcaca caagacacag tgtcaggttt tatatcaact
    10021 aagtggtaga taagtagaat attagcaata tcaacgagta tttagaaact tattttatat
    10081 taataaagca gtatataaaa ttgtagaatc aacaatagca tatactaaaa catccacctt
    10141 atattaacta aatcatatct aaataaaata cacttaccta caaacattat aaaatcaata
    10201 caaaaatgag gtgaaacaaa atttatgata aagaaattaa acagtaaaga tataaataaa
    10261 atcatggaaa tatgggaaaa aagtacaatc aaagcacatg actttataag taaagaatac
    10321 tggcaaaata actacaatac tgttaaaaac gaatatatac ctatatcaga tacatttgta
    10381 tatgatgatg gagatgaaat aaaaggattt ataagcataa tagataaaag atttatagga
    10441 gctttattta taaagcccaa ataccaaaat ctaggtatcg gaggtaaact tttagattat
    10501 gcaactaaaa aatataaaag tctaagctta gcagtatata aagataataa aaaagcagtt
    10561 gtgttttata ataaaaaagg ttttaatata gtaaaagaac aagtaaatga agattcagga
    10621 tttaaagagt acataatgga atatagtaaa taatatgatt acattctatt aaaaggataa
    10681 ttaatttatt tgccatttga ttttttaaga gtaattttta aaatagacta tatgaagata
    10741 aagatattta ttatagatgt aagttaatca gaattgatat agctacaaaa gaggaaaata
    10801 aaattgtatg gtgtatttca gatttaacaa atttactatt taaagataat aagtttgata
    10861 taatattaaa gatactttca cctataaact atagagtatt gaaagaattt taaataatta
    10921 tggaatgcta ataaaggttt gttaaaattt aataattatt agattataga aaattgccat
    10981 gaaattagtt catcaagaac tagtttcata gcaattttaa ttcttataag tgtatttatt
    11041 tttacagatt taaaaaactt atacagtatc taataatgta atcttattac tttagcctat
    11101 aaatatttaa atcttaaaat gttctctttg agataagctt atttcagagt cacaatttcc
    11161 acaacagtaa aatgttgaga catctttaaa tttttcagta ttctcatcaa agttcatttt
    11221 tttctttgca ataattccta ccttagttaa attagaacca caattattac agttttgaat
    11281 tttgcatagg ttatatagtt tatcaagtgt aatatcttct aataattcat cacaattaca
    11341 acatctgata gaagaaactt cactacacat ttcaataggt tcagcttctt tagaagaaaa
    11401 tttatatgtt atgtcatttg atactttata acctgtatca aatatattag aattacaact
    11461 tggacataag tactttggaa gatatatatc ttttttttca aaagtatcac tactaatatt
    11521 acagtttaat tcatctaaaa ttggtctttc atctaggtta ttacctttat ttaagcttag
    11581 aagataatta taaatttttt tatcttcatt ctgttttgat gtatctaaca aatgctccat
    11641 tatattagtg tattttttgt tttttgaagg catgtttaaa tccagataat tgttttcaat
    11701 agcaaagcat actatattga aatagttata tttaactgca aaaaataata acatttcatc
    11761 aaatacttta tctattattt tgtcagaaat acttatccaa aaatctggtg actctaagta
    11821 aactttccta ctaagttcat ctaactcttc atctatatta tttatattat tttcattaac
    11881 tttcatatca tcacttcctt ttaaatatct tgtgtataaa ctatttgtta ctatttttta
    11941 tataaaataa aatcttatat aaaaatatat catattataa tttacaagta tagtctaaat
    12001 agagctgttt ttaagcaaag ttttataaat gactattaaa atataaagta acagtataaa
    12061 gtttacttat aaattaaatg taaacctaaa taattataat agttgacaaa aaaacgtctt
    12121 tattttatac taaaacaaag ctttaataga agctttgtat atgtacatat aaactaaatg
    12181 aaataagaca tatgaaggga attataaaaa tgaaaaatac taaagtaaat attcaagatt
    12241 taactaaaat gtcaatatgt gtagcattat tgtgtatatc atcttacata tatataccat
    12301 tgccatttac tccagcagga gtaacagcac aaacaataat gataaattta atagctttga
    12361 ttttaacacc aagacaagca tttagtacag ttggtgtata tataatgata ggtttaattg
    12421 ggattccagt atttggtgga ggtacatctg gtattggaaa gttgttaagc ccaacaggtg
    12481 gtttttattt tgggtttttg tttgcagcac taattattag tttattaaaa ggacgaaaca
    12541 atgatattaa gagatatata ctaataacaa tttttgttgg aatgcctatt atatatttta
    12601 tgggaacagt atttatgtgc tattttaatc aaatgacagt agaagcagca ctttttgcag
    12661 cagtagtacc atttttaatt ggagatacaa taaagtcggt tattgcatct tttttaggca
    12721 ctaaactgaa caatgtactc agaagaaatc tatgataaat ctataatata aggaagggtg
    12781 taatgtatgg aggaagtttt tatattagga ggattgagaa gtcatatagg tctaaaaaat
    12841 ggaatttttc aatttgttca acctgaatta ctaggagcaa gtgttttaaa agatttaatt
    12901 aaaaagtatg aaattgataa aattgatgag attatatgtg gaaatgcagt tgggacagga
    12961 gggaatatag ctagattaat gactttaaca gcaggagtgt caaatgaagt acctgctttt
    13021 actgtggata tgcagtgtgc ttctgctatg atgagtatag acatagcttt ttcaaaggtt
    13081 aaatctggtc agtgtgattt aattattgca ggtggttttg aaagtagttc tctacaacca
    13141 atgcgcacat atcataagaa tgataagcga tataatacaa ataatccaaa ttatacagtt
    13201 gctcaatttt ctcctgatga taatagccaa aatagtatgc tcgaaggtgc ggaaagagta
    13261 gcagagttat atcaaattga gaaagcagac ttggattttt gggtaaagga aagtcataaa
    13321 agagcaaaag aagcaagaga ggaaaaaata cttgaagata ttatatctcc tataaataat
    13381 agtacctatg atgaaggtat acgtgataag atgagtcaaa ggttattaga taggatgcca
    13441 tcaattttag gaaaagaaac tattacaaat gcagcaaatt catgtcttat taatgatgga
    13501 gcttcattta ttataatatg ttctaagaag tatttagaac atgtaaaaaa gaagcctaaa
    13561 gcaaaaatta ttaatacatg cacaatagga acagatgcaa ccttaagtcc aacatcagcg
    13621 atacaagcaa tggataaact tttagagata gaaagtctta attatttaga tgtatctgct
    13681 gttgaattta atgaagcatt tgcagttata gatgtgttgt ttcaaagaaa atatccagag
    13741 ttaatagata gatataatat atttggtgga gctttagcgt atggacatcc atatggtgct
    13801 tcaggtgcaa ttattgcatt acatctttta aaagcattag aaaagactaa gggtagatat
    13861 ggaatttgct ctatagctgc agctggtggt ctaggttcag ctttactgct tgaaagggtg
    13921 tagattatga actattatga acttttaaga ataaaaaaag agaggcataa aaataaaaat
    13981 tttttaatta tagatggaga aaaatatact tatgaaaata tattgagtta tagtgaggaa
    14041 ctaggaaaac aaatttcttt aggtgaaaat atcccaattt taatatattc taaaaatata
    14101 atgtttcagt tgattagttt ttttgctatt aattattcaa aaaatattcc tattatttgt
    14161 cattataatc tttctaaaaa agtattagat gatattttat taaaaaataa tatttcaatc
    14221 attatatctg atgaatctat ggacgttatt gatttatatg atgataacaa taatgaagta
    14281 gcaaaaaaag ttaatttttc tatttttaat cctaaattta atatatatat gtatcaatat
    14341 aagaacctta taaattattt agataaaagt atttgtatgg gagctttatc ttcaggttct
    14401 acaagtgttc caaaggtatt atatagaaca tatgagagtt gggctggatt ttttcctata
    14461 caaaatggta tttttaatat ctctggggaa tcaatcttgt ttgttaatgg aacacttagt
    14521 tttacaggga atctaaattc tataatgtca gtattatatg aaggaggtag tattgtaata
    14581 agttcaggtc ttaattgtaa atcatggata aggataataa aacaatataa tgtaactaac
    14641 atatacctta tacctacaaa acttcaactt ttggttaaac acttaaaaga accagtactc
    14701 aaagttgtaa gtatttttac agggtctcaa ttattatttg aagatactgc aagaaatcta
    14761 aaaaaatata tgccaaagtc tgaaattatt ttatactatg gggcaagtga attaaattat
    14821 attacatatt tatgctatga tgaactaatt gaaaaacctc taagtgtagg cagaccattt
    14881 ccaggaatag atatatatat tagagaagga aaaatttttg tgaatacaga atatgcagta
    14941 tataatgcaa ccaaacctta ttctgtcaat gatattggat attatgataa tgatggatac
    15001 ttaatatttg aaggaagaag tgatgacatt ttaaatattg gaggatttaa agttagttct
    15061 actaagattg aaaatgaagt taaaaaaatt cctcaaattg aagatgcaat tgtattgccg
    15121 tactcagaca ctataagagg aaatcaaatt gtattatttg taataactat agacaaaatt
    15181 acgaagaaag atttgttgat taaaatgaaa gataatctaa tgaaaaatga aatcccaaag
    15241 aaaattatct ttttaaattc atttccatat acctcttctg aaaagataga tagattagca
    15301 ttattaaaaa tgttataagt ataatattta gtaattttag tgaaccaatg tattgataaa
    15361 aacatattgt atatagtaaa ggaggcttac aatatgtcaa atcaatacga tataactgtt
    15421 attgggggag acctgcgaca aatgtacatg gtaaaaaaac taattaataa agggtattct
    15481 gtagtcactt atgggatatc aaatgaaatt ataaatggaa tcgttggaaa atcaacttca
    15541 ctagctgaag cactatccaa aagttccatt attttatgtc caattccatt tacaaagaat
    15601 caagttaata ttgaaaatac ggatgattgt cttgatgcta ctattgataa cttgctaatt
    15661 aatttatctc ctaatcaaat attatttgga ggtaacatat caactaaagt ttgtaaattt
    15721 tgcgagcaac actctattca gatttatgat tttatgaaaa tgaatgatgt tgctatttta
    15781 aatgcagttg ctacagctga aggggctata gcagaagcta ttaaaagaag tataatcaat
    15841 atacatcaaa gtaactgttt agtattaggt tatggaagat gtgcacaaat acttgcaaaa
    15901 aaattatacg ctttggatgc aaatgtatgt gttggcgcaa gaaataatga tgcaagaagt
    15961 acggctaatg catttggata ttccagtatt tctttaagtc aacttgatga aaaactttta
    16021 caatttgatt atatttttaa caccatacca gagtgtattt taacgaaaga aaggttagaa
    16081 aaagtatcac aagaagtaac tattatagat atagcttctt ctcctggagg agtagattat
    16141 tctgttgtta aagaagcagg gattaatgcg actctttgcc ttgggcttcc tggtaaatat
    16201 tcaccaaaga catctggtga aattcttgtt aacgctatag aaaatattat aaatgaaaga
    16261 agggattaga aatgagactt gaaggactta caattggagt aggatttact ggttcgtttt
    16321 gtacttatga taaaatattt atagagttag agaatttggt taaagaagga gctaatgtac
    16381 acactatatt ttcagatgta tcacaaaata tcgattgtag atttggaaat tctgaagagt
    16441 ttatgaaaaa agcatatgaa cttacaggaa ataaacctat agttacgatt gaagaggcag
    16501 agccatttgg acctaaagga attgctgata ttataataat agctccttgt actggaaata
    16561 ctgctgcaaa attagcaaat ggtattacag attcaccagt attaatggca gcaaaaggtc
    16621 atcttcgtaa tgataaacca ctagtaatat caatatcaac gaatgatgct ctaagcttta
    16681 atttcaaaaa tattggcatt ctccttaatt ctaaaaatat atattttgta ccttttggtc
    16741 aagataattg caaagcaaag cctaattcta tgattgcaca tactgagcta ataattccaa
    16801 ctatagaact tgctctagag aacaaacagc tacagcctgt tataaaaagt ccacatcaat
    16861 agaattggtt cttataatat tatgtattag ataaaatact agcaaagagg ctgtctcaaa
    16921 atagagagaa gcctcttttt cttgttgaaa tgatgaaaat atatttaatt tcatacaaaa
    16981 aaatgcctca tggactaaaa agtctatttt gagctaatct ccgtaatttt tggtatatta
    17041 aaattattta cttaatccaa tttttaaatt agaatttata tcgttttgac tcttcaaata
    17101 tcaatctaac tgttgttgat acaaaagaag aggacataat taaaacaaat accgatagtc
    17161 ctctaataaa caaattatct acataataag ttactattcc aatacctcca atcgttaaaa
    17221 ttacacttat aatcaaagaa aaaatgctaa ttgtttttaa ttttttcatg cttatcctcc
    17281 ttcttataaa cttcaaattt atctatataa tattaaatat attttatttc atagtatata
    17341 aaaatcatga ata
//
LOCUS       DAMJYC010000086        17281 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJYC010000086 DAMJYC010000000
VERSION     DAMJYC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.088"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            33..779
                     /locus_tag="PC472_002994"
     CDS             33..779
                     /locus_tag="PC472_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2728303.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            840..1514
                     /locus_tag="PC472_002995"
     CDS             840..1514
                     /locus_tag="PC472_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2728304.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKREFIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            1883..3229
                     /locus_tag="PC472_002996"
     CDS             1883..3229
                     /locus_tag="PC472_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2728305.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMYYSQMTARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEYIDAKVLAGESMYYYKKIKD"
     gene            3256..4650
                     /locus_tag="PC472_002997"
     CDS             3256..4650
                     /locus_tag="PC472_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2728306.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDIKMHKPEVGDIISTIIKDKSILSAHYGGTWEKIGEKI
                     EFGITVHYYKLIEK"
     gene            4860..6296
                     /locus_tag="PC472_002998"
     CDS             4860..6296
                     /locus_tag="PC472_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2728307.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKDMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            6640..7584
                     /locus_tag="PC472_002999"
     CDS             6640..7584
                     /locus_tag="PC472_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2728308.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRF
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7753..8685
                     /locus_tag="PC472_003000"
     CDS             7753..8685
                     /locus_tag="PC472_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2728309.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8790..9257
                     /locus_tag="PC472_003001"
     CDS             8790..9257
                     /locus_tag="PC472_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2728310.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9501..10355)
                     /locus_tag="PC472_003002"
     CDS             complement(9501..10355)
                     /locus_tag="PC472_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HDF2728311.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSFKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSYIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VLMLSIIK"
     gene            10521..12485
                     /locus_tag="PC472_003003"
     CDS             10521..12485
                     /locus_tag="PC472_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2728312.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSANPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12485..12946
                     /locus_tag="PC472_003004"
     CDS             12485..12946
                     /locus_tag="PC472_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2728313.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12949..13269
                     /locus_tag="PC472_003005"
     CDS             12949..13269
                     /locus_tag="PC472_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2728314.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13285..14385
                     /locus_tag="PC472_003006"
     CDS             13285..14385
                     /locus_tag="PC472_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2728315.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14505..17105
                     /locus_tag="PC472_003007"
     CDS             14505..17105
                     /locus_tag="PC472_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2728316.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6855 a         1780 c         2999 g         5647 t
ORIGIN      
        1 aaaaatgtaa actgtgaaat ggaggtagta acatgaaaat tgaaaatctt tctattgatg
       61 atattaaacg aggctatata tttaatgatg atttaaattg ttatagttgc attaaatgta
      121 caaaaacata tgaggtagga gaaatatata aaataggtaa tagattcttt gaagcttcaa
      181 aagctattga acttcatgtg gaattagaac atagtgatta tttagagcaa ttgcttaata
      241 gtgattcaaa gtacaacaca ttgactgaaa atcaaaaaca attatttcaa ttatttatta
      301 gtgatttacc agataaggag atagcaaaac aggttggagt atccacatct actatacgcc
      361 atcaaaaatt tattttcaga gaaaaagcaa aacaggctaa gttctattta gcactatatg
      421 aatctgtatt tgaagataaa tctaataaaa gtagtactat aataccaatc catgaacatg
      481 ctcgaatggt agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac attttggata
      541 catcatttga aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa gaaaagaaaa
      601 aggtcgtaat tttatctaag attttggaac aatttgaacg tgggaagaaa tattcagaaa
      661 aggaagttaa tcagatttta gaatctatat atgatgattt tgcaacaatt agaagatatc
      721 taattgagta tggttttatg agtagaaata aagaatgtac aaagtattgg ttaacataat
      781 taaaataata aattttacta atgtgtaatg ttatttttat ctagggggag gagtcaaata
      841 tgaatgatga attaaaatct attttataca ataaaggtgt agacattgtt cgctttgttg
      901 atatatctga atttccaata aatcaaacac aaggtttttc taaagcaatt ttattttgta
      961 tagggttatc aaaaaaattt attacagata tatacaataa cttacctaca gatagtgatg
     1021 agtttttgga aaaagaggag aaagtagaag aattagctga ctggatttca aaatatattc
     1081 aaaataaagg atatagagca tattctcaat cggagaaaaa caatctagaa catggatatt
     1141 ttgaaaaagc ctatattaat ccagaaatgc aatcagggat tagtccttta ccacataaga
     1201 caatagcaaa tattagtggc attggattta tgggaaaaaa taatttgttt gtaacagagg
     1261 aatatggatg tgcttttagc atgtgtacag tacttacaga tgctccaatt tctgttgaaa
     1321 gatatccttt gattgattca aaatgtatgg attgtaatgt ttgtgtagaa aactgccctg
     1381 caaaagctat acatggaaat gagtggactt tgccaggaaa aagagagttt attattgatg
     1441 tttcaaagtg tttttgtgta ttgaagtgta tgatgagttg cccatggtca ttaagatatg
     1501 caaatcaaaa ataattttac tagagaaggt attaaattga atattataaa atttttaaaa
     1561 tgtaagatta tataagattt ttcaaaataa aaatttgtat agaacactca gaaatgagtg
     1621 tttttttatt gggattaaat tatattgata caaagcttaa ctattttaat cattatataa
     1681 aatttgtatt aaaaaattaa gaataacttg gtaatagtga aaatataatg agattataat
     1741 tgatttataa tataactata atgatattgt aaaaatgaaa attgtcctct caaaattccc
     1801 aaagagagta aaagtcactt cagttatagt caaaaaaatt tttctgtgaa aagaatttag
     1861 atttgaaaag gaggaattaa tcatggaaat tgaaactaaa actccagatt ggattaatgt
     1921 actagaatta aacagtaaaa tagatattac tgggaaatat gatgtaagta atatgataca
     1981 aaatattgtc tctgataagt cactagaagg ttccattata tattttccaa aaggtaatta
     2041 cttatttgag aaaggtatta aaattagtca acaaataaca cttcaaggag attcttatta
     2101 tggaggaggt aatcaagtat caaatttaga taaaaaacaa tcaatcatag gaacaaccaa
     2161 ttttataacc cgaggagttt ctaatatgag tattattaca ttgtcaggaa caagtcaatg
     2221 tatcaaaaat attaattttt attatgatag tcatgatata gagaaaatac cgcctaaaaa
     2281 tgtatctgct attacagaat atggagaaac acaaggacta tctcattttg aacatctgtt
     2341 tatttctgga ttttcaggga taggaattga aattccatac tattcaactg gtaatgatat
     2401 tactgttagt tcatgtggtc ttggtatgag gcttggagag aagtcaatgc tttctagtag
     2461 caagatttac gaatgtaaaa atggaatgga gattactact ggtgttagct taaataatgt
     2521 gagaatagaa gaaataaaag aaataggtat taataataaa ggatttggtt ttaatctgat
     2581 tatgaatctt atggtcgacc aatgtggata ttgtggattt atgtttgaaa aaatgtacta
     2641 tagtcagatg actgccagaa ttactagatg cggtcagtat tataaaagtg ttgattataa
     2701 tgcttatgag aatatggaag atagaaaaga agaagcttat tccatttttt atggagatat
     2761 tttagaaaat tgcaatattg tattgatgaa tagtaatatt gataccttgg aaaatgattc
     2821 acttaatgaa cataagatat atgtaatcaa ggcaaatgaa acaaacaatg taatgctaac
     2881 atgttatgct gaagcagata actttataca atgtgcaaaa ggtaacttgc tgttagaaaa
     2941 tggtgaaaat acttataaat tttataatgg tgagatttgt tctgtaggag gtgttcaaat
     3001 ttctgatata gatgaaaaag atttaatcaa aataatcgag aacactctgt atatagatga
     3061 taaaggtaag tatttaatgg ttcctagatt aaaaaagaat actgtaatat attcagtact
     3121 agattctaca gaagaaataa ataaattaca tggtggaata tgggaatata ttgatgcaaa
     3181 agtattagct ggggaatcca tgtactatta taaaaagata aaagattaaa ttatcaaact
     3241 aggaggtagt gattaatgga agagtctata ataattaaag atataagtgg ctggattaat
     3301 gttcttgatt atggaatttg taatgatgaa acaaaagatg taagttccaa gttgcaagag
     3361 attatggatg ctgtaccaga agattctatt atatattttc caaaaggaaa atacttattt
     3421 gaaagtggtg ttaagattaa gaaacgatta actttatgtg gagattctta tattttggcg
     3481 aatgttccta aaaatactgc tggagtaaca caatttactt ttaaaggaaa gaaaaatcct
     3541 aaaagtgaaa atatagcaat tataacagca aaaagtgtaa gacactgtat taagagcatt
     3601 tgcttttatt cagatagttg tgatagagat atgacatcta atcatgaacc tccaacagat
     3661 ggaaaaccta gatttcatca taatgtaatt ataaattata gaaatatatc ggccattgta
     3721 tgtgaagata aacagggaga atcaggtcat tatgaaaatc tatcttttag tggtttttct
     3781 ggaattgcac ttgatatgcc taataattct acagcaaatg atattacaat ttttacatcc
     3841 ggacttggaa ttagtacagg agaaaattca actattgcta acagtaaggt ttggggctgt
     3901 gttgatggaa tgataatttc tactggaact tttataaatg gtatgagggt agaagaaata
     3961 aaaaaagttg gaattaaaaa tattggtaga ggtttaaatt ttattacaaa tatcacaatt
     4021 gaccaatgtg gatattgtgg agtttggttt gattctatat ccaatgttca gatatcagga
     4081 aatatcacaa ggtgtgggca gtactattat gatgtaagct atgataatta tttaatgttg
     4141 gataatagag ttaaagaagc atattcttta ttctatggta atttattgga atcaagtaat
     4201 atagttttat caaataccaa taaagataat tgggaagatt tcaaagaaga taagcataag
     4261 gtgtatcttg tagaagcact tgaaaccaag aatgtactat taacatgcaa tatagatgct
     4321 actgactgga ttgttaagtc agaaaaagga aatttaacgt acaataatat gcgacaaact
     4381 gttatatttt acgatggaaa actaagtagt atcaatggaa ttggtatatc tgatgaagat
     4441 tatggggata aagtcaagat aaaaaaagga aatatatata taaatagtaa ggatattaag
     4501 atgcataaac cagaggtagg tgacattatc tcaacaatta ttaaagataa aagtattctt
     4561 tctgctcatt atgggggaac atgggagaaa attggtgaaa aaatagagtt tggaataact
     4621 gtacactatt ataaattgat agagaaataa gtaaatacgt agtatttgaa tagatgtatt
     4681 ttattggaga gctaggacta tagttttaat gtaaattgtt tggtaatagc atggattgaa
     4741 taatataaaa ataatataaa aaattaattt gattatgtaa atttctatga agtagtaaaa
     4801 ctgtgacttt agtcatagga ggttcaaact aagtaaaagg gaaaggaata tataatatta
     4861 tggaaggaag aactgggatt aaagatggaa gatggattaa tgtagtagaa agaggtatta
     4921 aaaatgatgg ttcgtacaac gtaggtcctg ctttgcagga tattattgat gaaatagaaa
     4981 cagatttttg tgtcttatat tttccaaaag gtaaatacct atttacaagt ggtgttaaaa
     5041 ttaataaaga aattgtattg caaggtgatt ctaattcatc tacaggtact acacaattta
     5101 taactcgtgg agtagaaaat atgagtatta ttacattgac agggaagaaa caatgtatta
     5161 aaaatataaa tttctattct gatagttgtg agatacaggt caatgaagaa ccaccgacaa
     5221 aaggtaatcc taaatatcat tatgagatga ttatcaataa gaatgaaaag aatgttgaga
     5281 tgaacaatgt atcagcaatc ctgtatgata acagtaagga tatgattaaa ggtctaggtc
     5341 attatgaaaa tatttatata agtggttttt cgggtacagg aattaggata ccatattact
     5401 caattgtaaa tgatattaca gtttcatcct gtgggctggg tattgataca ggaatagaca
     5461 caattatttc taatagtagg atatctaagt gtcaaaatgg aatgaggatt agaactggaa
     5521 ctagtataaa taatgtaagg attgaaaaaa tacagaaagt tggtcttata agtagtcaag
     5581 ttaaggggta tgaggggtat ggaagttata aaattaataa tattacgatt gaccaatgtg
     5641 ggtattgtgg cttttcattt gattcaatga aagatagtta tttttctggt attataagaa
     5701 ggtgtggtca gtattattat aatacagatt atgacacata tttaagtatt aaagatagag
     5761 tagaagaagc atattcttta ttttatggaa attattttac agaatgtaat attgatttag
     5821 ttagtgataa taaggataac tgggatgatg gcttagaata taaacataaa gtttatgtat
     5881 ttaaaaccct tcaaatacca aaattaacat tgagatgcaa tatagatgcc tatgattata
     5941 tcgttaaatc ggtacatgga ggaggtaatt taatgttaaa aaataacttt aacaattata
     6001 tatttattga ttcatctagt ccagaagtta ttaatggaat taattttaat tatcaaaaga
     6061 atggaaacct gttaaggtta aataatggta aattaagtta taaaaatgga aaggaagaaa
     6121 cagctatatt cagacctaat tatggtctta ttgtagcatc aacagattca gaatgtgaac
     6181 aaatagaggc tacctatgga gaaaaatggg agaagcttgg ttctgaagta attggaaaag
     6241 aaacggtata ttattataaa catcatgaac cagaaaaaac aaaccaaata caataaaatt
     6301 tgtattatat aatgatataa agttttaata tatagtgttt acctttaatg taatattgat
     6361 atatttgtat agtattaaat ttgttaaaaa gtaaaattgt agggtgcttg aaaatagcac
     6421 cttttttagt ttcattataa aattagtttt gaaagtttag gaataatgta tatttatttt
     6481 taaagaatta taactttatt aaggatattt taaatgctag aaatgaatca gtaaatcata
     6541 ctggaattat cttaaatgtt ttgattaaat taagtattta gagctataat tatattaaaa
     6601 atatattaga acataattat attactatgg aggttaaata tgatagtaag tgtgagtaga
     6661 agaactgata tacctgcttt ctatagtaag tggtttttta acagacttaa agaaggtttt
     6721 gtttatgtaa ttaatccaat gaatccaaaa caggttagta agatagagct aaatccacat
     6781 actgttgatt gttttgtatt ttggacaaag gatgtaactc caatgttata tgatttaaat
     6841 aaattaaaag actataaata ttattttcac tatactataa catcatatgg aaaagaagta
     6901 gagacatgta ttttagataa aagaaaggta atagattcat ttaaggaatt gtctagaaaa
     6961 ataggcaaag aaagagtaat tttaagatac gacccgattt tcttaagtga aaagtacaca
     7021 gtagattatc atatcaaagc atttgcgagt ttatgtaatc agcttgatgg atttacagaa
     7081 aaatgtataa taagttttat cgatttgtat aagaaaacaa aatttaatac taaagcatta
     7141 catataaagc caatagaaat tacagaaata gaaacattat caaaagagat ttctaagatt
     7201 gctcataagc atgggataat acttgaaatg tgttctgaag aatataattt aagtaaattt
     7261 gggattggaa aaagtaagtg tattgatgat agattaatat caaaaattat aggatgtgaa
     7321 gtagaggtaa aaaaagatag tacacaaaga gatatttgtg gatgtgttaa aagtgtcgat
     7381 ataggacaat acaatacttg cagacattat tgtctgtatt gttatgctaa ctttaattat
     7441 gcaaaagtag aagaaaactg taggttttat aaggaagata ataaattact catagatact
     7501 ttaagagatg atgcaaaaat aagcattaga gatatgaaga ctattaaagt agataagaat
     7561 aaacaaatta gtatatttga ttaaaataaa tatactaatt accttaatat attaagaata
     7621 tagatacttt tgataacaaa attaaactta atagatattt tctatataag aaagatggtc
     7681 ataatatgtc aatttgacca tctttttgat ataattaaaa cacaaataat ttagtaacaa
     7741 aggaggcaga acttgaattt attaaaaaaa tatcctatac ctattgcaag tttgatttta
     7801 tcaattttta ctttaggtaa cttattacaa tcttatagtg aatcattacg taatgtaatt
     7861 ggatttatag gatttatttt gtatgcaatt tatatagtca aaataatatt acttagaaag
     7921 ggtgttaaag agcaattaga gaacccttta atatcaagta catttccaac tataacaatg
     7981 gcaacaatga tatttgctac atatataaaa ccattatctt tagaattaag tattatgatt
     8041 tggagcatag gaattgtggg tcatcttgtg ttaatagtct tatttagtaa gaagttctta
     8101 gttaaattct caataaaaac ggtatttcca tcttggtata tagtatatgt tgggatagtt
     8161 gcagctagtg ttacggcacc agcatttaat caacttttga ttggcaaaat tgctttttgg
     8221 ataggatttg tatcttatat agttcttata ccagtaattt taaagagagt atggataatc
     8281 aaggaaatgc ctgaaccatc tttaccaagt atagctatat tagctgcacc agggtcacta
     8341 ttattgtctg gatatattaa ttctttttca gataaaaata atgtagtatt atacttgtta
     8401 tttatattat caattgtatt ttatattata gttttgggat atttaccaaa attattaaga
     8461 ttgccttttt atccgagttt tgctgcattt acattcccta tggcaataag tgcaatgggt
     8521 atgaaactaa tgaatggtta tttaactaaa tcaggtcatt ttataagttg gatttcatat
     8581 gcttctaagt tagaagagat aattgctaca gtgattattt tatatgtttt agttttgttt
     8641 attatatatc ttagtaaatc tgaaactaca tctaaagcta aataagaatt attaaatatg
     8701 tgtatttaat aggaatattg atataaatat atgaagataa gataaaccat agaaacttaa
     8761 tttagcttaa ttttattgga ggaaaacaaa tgaaaacaaa agaagaatat ttaaaagaag
     8821 ttagaaacat atcagaagaa tattttagaa aaggagagtt tttttgctct gaagcagttc
     8881 tccagacaat aaatgatgct ttaggccaac ctttaagtcc agagataact aagttagcat
     8941 caggatttcc gataggactt ggaaaggcac aatgtctttg tggagcaata tctgggggag
     9001 agatggcttt aggtatagta tatggaagag ttcatggtga agctatgaat ccaaagatgt
     9061 ttgagcatgc taaaggtttg catgattata ttaaaaaaga gtatggtgca acatgttgta
     9121 gagctattac aaagaaatgg gatggtgata actttatgtc tcccgaaaga aaagagcact
     9181 gtattaaaat aacaggtcaa gttacagagt gggttgcaga taagcttata gaagatgaaa
     9241 aacttaatat caaatagtgt aatattagtt ggtgaattta taaagttaaa tttacttata
     9301 gtataagata cttgatattc tcgtattgat agaaaaattt atagcatatg tatttaatga
     9361 aatatactta tatttagagt gtggtttaat ttcataaata catatgctat tattttaagt
     9421 atataagcaa atttatttaa ataaatagaa acagttaatt tatatgtaca aaactatata
     9481 tgattagtgt aaataaatac ctattttata atactcaaca taaggacacc agacatgatt
     9541 agtattatac caataaaaat agcaagccat tcttttttgt tcttcttttc tttaaaaaat
     9601 ataatccctc ctaaagtggc tactatcaca caactctgag agatagaaaa acttattgta
     9661 actcccaatg tattagtcgt tatgaacata cctaaatttg ctattgacca caatatacca
     9721 gtaattaaat tgaataaaac attttcccta tatacaatct ctttagaaaa atacttgaca
     9781 ataatatatg aagttatcaa cataccaaca gattgaggta atataattct atatccttca
     9841 attaaaaata tttgattagt tacaacatat aatgataaaa atagagatga taataataca
     9901 actccaacac attttaacat atttttatct gtagattttt tagtttgata atttgtgaga
     9961 agtataccaa gtattataca taatattgat aaagaaccaa tcagtaacat tttaaaactg
    10021 gtccattcat taaacaagat tacagctaat aatgtcgtaa aagttaattg tgaaccattt
    10081 gaaattggca ttactttaga tacactagca atttgaattg ctttaaactg cattaattgt
    10141 cccaaactcc agaatatgcc agaaatcata cttatacaaa atgaaaaaaa tgtaatttca
    10201 ggatgtataa ccagagttat tatagtggaa aatataaacg cacttatgga tgtacctaaa
    10261 agctgttcat gaggagaact tttctttaga tttgcaacta tgggcatcaa tccccatccc
    10321 attacaggaa gtaaagcaat aattattgtc agcattaaac tatttccttt catattaaat
    10381 ttagacagct tttaatttat catctaaaat taaagtgttc aatatataaa aattaccgta
    10441 tttaatcagg aaatattagt agttttttag aaaaacattt tccattaaaa taaagacaaa
    10501 gggaaggagt aaaattgaaa atgttaactg atatgattca aaatcgtgaa attaaaataa
    10561 taaagttttt attaaaagaa ggtcatacaa ctgtaaaagc aatagcagat gatttagatg
    10621 tttcagaaaa aacagtttca aaatctctta aagaagtaga agcagtgctt aaaaagatag
    10681 gtctttcttt aataagaaaa cctaagatag gtgtttatat tgatggagat ttaaaaaaaa
    10741 tacttcctta cctagataat aaaggaagtc aaattcctac aaccagagag gagagagtta
    10801 tctatatata ttcagtactt ttaaagagta atgattatat tactatacaa caactttctg
    10861 atgaactata tataagtcgt ggaactattg aaagggattt agcagaagtt gaaaaactgc
    10921 taaaaaacga agggatttcc atatataaga aacctagtaa gggaataaag ttaaacataa
    10981 gtgaaaggga taaaagacaa cttactgcta agtttataca taaattttgg agtagaaact
    11041 ggtatattaa gcaagagcaa gaaagctttt atcaagcatt tgaaaagata caggcagatg
    11101 taaatggtat tttttcggaa gatgatttat caataattat tgatattctt aggaatttca
    11161 gcagagaaag aaaatttgag ttctctgatt atgaatttca gtcattagtt attcatttgg
    11221 caatagctgt agagagaatt agaaaaggcg aatatataga agaaagtttg tatattggta
    11281 atatagaaaa taatcaaatg tcaaatacca attatttagc aaaacaatta gaagaaaaat
    11341 tttcaatatt attaccaaaa tctgaaattg gatatataaa tttacattta gtagcggctg
    11401 accaacatag agatactaat gaactaaaaa atacttcagc taatccttgt gagacaagta
    11461 caaataatat atcaaatctt cgggaaatta taaaaaatac attgtatgag acagcttatg
    11521 acaaggattt aattgatgga ttgacttcac atgtacattc atcaataaat agactgaaat
    11581 atggacttag tattaaaaat ccatatgtaa atacaatcaa acaaaacttt tctttatcat
    11641 ttgagcaagc tttagattta aagaaagtaa ttgaatttat atatgatatt aggataaatg
    11701 atgatgaatg tgcttatata gcattacatt ttgaagcttt tagagaaaga atgaaagaac
    11761 ttccacaaaa tattagagta attctagtat gcagtacagg attaggttca tctaggttat
    11821 tgtctgcaag aataaaaaaa tactttccaa tgataaagat tgaaaaagta ctttccgtac
    11881 aagaattagt atccagtaaa attgatgcag atttagtaat aagtactatt tatttagagt
    11941 tagaaacaat tccaaccata gtagttagtc ctattttaaa tgaaagtgat ataagaacct
    12001 tggaaagaaa tatacaaaat gtttcaagtt gtacttctaa gaagtataaa tctttcagaa
    12061 atttaatctt tgaagaaaat atattgttga atattgaagc ttcaaattat gaagatgcaa
    12121 ttaatcatat aacagataac ctaataaaaa aaggttttgc aaaagaaggt atagctaaaa
    12181 gtgcaattga gagagaaaat ttatcttaca cgtctttcca gtcaatagct acacctcatg
    12241 ctaatcctag ttatattaaa aaatctaata tatctgttgc aacgttaaaa aatcctatta
    12301 catggggaaa tgaaaaggtg gaagttattt tctttatttc attagaaaat gacaaaaacc
    12361 tagagcttga tgaaatatat gaagtttttt atgacttcat tgacaataaa aaaaatataa
    12421 atgacatatt aagtgcaact tccacaaagg aagtgtacga tttgctagtg gaggagagta
    12481 tatagtgaaa atatcagatg tattgaaaaa agagcaaatt gtttttaatc ttgatacaaa
    12541 tacaaaggaa gaaacaataa aaaaattaag tagtatattt gttgaaaatg ggattgtgga
    12601 caatgaagaa gaatatgtta agtctgtttt ggaaagagaa gaacattcaa ctacaggtat
    12661 tggtaatggt attgctattc ctcatgggaa aagtaatgct gtcaaaaaat cttctataat
    12721 atttgcaaag actaagcata agattgaatg ggatgcttta gatgaaaaac cagtagatag
    12781 tattttctta ttggctataa gcaatattga taaggatagc aatcatcttg tactattatc
    12841 taaattagca actaaactta tggatgatga caatgtagat gcactaaaat tagccaattc
    12901 agaacaagaa ataatcaata tatttagtga agaaggagag atgtagaaat gaaaagaaaa
    12961 attatagcag ttacagcatg tgcaacagga gttgcacata catatatggc agcacaagca
    13021 ttaaagaaag cttcaaaaaa gatgggtcac cttataaaag ttgaaacaca aggagctaca
    13081 ggaatcgata atgagttaac tacaaaggat gcacaaattg cagaagtagt aatatttgca
    13141 gtagatacta aagtcagaaa tgctgaacgt tttgagggaa aagaaatact agaagtacca
    13201 gttaatgcac ctataaagga tgcagagaaa gtaataaatg atgcattaaa gttaattgac
    13261 actaaataaa tggagggaaa tgtaatgttg aaaaagatag tctctgagtt aaaaaagcat
    13321 gtcttaactg gaatctcata tatgattcca ttagtaattg caggtgctat gataatggcg
    13381 atatctcgtg taggtggttc tttctataat ataccagata tatgggatgc taaatatgca
    13441 gaaagtgcta gtagtattgt tagattatta catgatttag atgggtttgg aggtactgcg
    13501 ttaggattaa tgtttcctgt aattgctgca tttataggat tttctatagc tgataaatta
    13561 gctattgtac caggtcttgt tggaggtatg attgctaaag atataggtgc aggattttta
    13621 ggagctttgg cagttggtct tatagcagga tatacatgtt tgtttattaa aaatcatgtt
    13681 aaattaccaa aatcagcagc ttcaatagta ccaatattta tagtaccagt atttgggaca
    13741 ttaatcactg ttgtattaat caattatgtg ataggtatac cttttgccac attaaatact
    13801 ggattagaaa attggttaaa tggattatct ggtacaaatc agatactaat ggcagctatt
    13861 ataggggcta tgatggcagt tgacttaggt ggaccagtta ataaagcagc tttaacaaca
    13921 tcacttgcat tattaacaag tggaatttat gcgcctaaca ctgctgcaat ggtaggaata
    13981 gttataccac cactaggatt gggacttgct actatactag caaaacaaaa atataataaa
    14041 cagttacgtg aagctgggaa atcatctctt ataatgggtt taattggagt aagtgaagga
    14101 gcaattcctt ttgcagtaga atctccatta aaagtaatac cttcatgtgt aattggaaca
    14161 gcaattgctt cagctatggc agttggttta ggttcagtaa atgcaactcc aattagtgga
    14221 ttttatggat ggtttacagt agagaattgg ccaatgtatg tgttatcaat agcagttgga
    14281 acagtaattg tagctggatt agttatagta ttgcgtggaa atccagtgat tgaggatgat
    14341 gaatttgaag atgatgattt tgatgaagca gagtgggaat cttaaaattt aataactatt
    14401 taagacgtgt tataagctat attcaatatg aatcttatac taaataaagt gttaaatatt
    14461 cagtttagac ttaaatataa aagataaaga acagaggaga ataaatgata aaagcacata
    14521 ttgttaatca tacacactgg gatagagaat ggtattttac ttctggagat gcattagtac
    14581 ttagtgaaca actatttaca gatgtaattg atgaattaga acgtaatcca gatgtaagtt
    14641 ttgtacttga tggacaactt tctattttgg atgattatgt acaattacac aaagaaaaaa
    14701 tagaaatcat taaaaagtta ataaaagaag atagactaca tattggacca tggtttactc
    14761 aaactgatgc attctttgct agaggagagt ctattttaag aaacttaatg attggaattt
    14821 ttgaaagtaa aaagtatggt aagtatatga aaattgggta tttgccagat acatttggat
    14881 ttaatgcaca gatgcctata ctattaaagc atgtaggtct tgataatata ttcttttgga
    14941 gaggtataca tcttggtaaa caggttcaat caccatattt taagtggaaa agcttaaatg
    15001 gagaaagtta tatatatgca gtaaatatgc cacatggata tggaactggt atgcttttag
    15061 agccttcatt aaagtatgtt aatggacgct tagacccagc tattgatttt attaaatcat
    15121 atactgatgt agatgaggtg ttaatacctt ctgggaatga ccagttaaat ataataggtg
    15181 atttcaaaaa taaaattaaa gcgattaatg atatcggaaa gtattcatat ataacgagtt
    15241 catatcaaga gtttttaaaa tatataaagt ccatagaatc actagaaagc tatagaggtg
    15301 aatttagaga acctgtatta gcaagaatac ataaatcaat tggttctagt agaatggata
    15361 taaaattagc atgtgataaa ctagaaaaca agctgattaa aagaatagag ccattattag
    15421 taattgctaa aaagagcaaa attgaaataa gcaatcagtt acttataaat acatggaaaa
    15481 aacttttgga aggacaagca cacgatagtt tagctggatg tgtaactgat actgtaacag
    15541 atgatattct tcaccgtata agagaagcaa atgaaatatg tgacagtatt gaaaatacta
    15601 ttgtaaaaaa gatttcagaa gggcttaaat tatctaaaaa tgatatttta gtatttaata
    15661 cagaagctaa gcgttttaat ggatataaag agatacaagt tgtaagtgat agtaaaaata
    15721 tttattttaa agataattta gatgctacta taattgaaga aacttatgta aaaccgagag
    15781 aaaatgtatt agaagaaaca cctgctggta atatatttat tgaagaacca ggatattata
    15841 tattaaaagt aagaataaat gtacagcttc ctgcacttgg ttataaagtt gttagttttg
    15901 aattaagtga taaagaaatg gtgtcacttg ataaatctaa tgacactttt atatctaatg
    15961 ataattataa gataatatat gaagatggaa gcttaaattt acaacttaaa gatggaactt
    16021 acatatcaaa ctttttaaca ttaaaagata gtggtaatgc aggtgatacc tatgactttt
    16081 ctccactaaa aaatgatgag aatataaaac tttcgtttga taaagtgtat acagaaaaag
    16141 ctttaggata tgaaaaaatg gtggttaaag gtactacttt actaccactt acattagaag
    16201 atagaaaaaa caaatatctt aatggtaaat taaatgtaaa agtaagtatt gtcttatcta
    16261 aagaaaaccc attgatggat gtaaaacttt gtgttgataa cacgatatat aatcatagac
    16321 tacgtgttca tataaaaact gatataaaag ataataaaaa catagcatca ttgccatttg
    16381 gatatataac aagaaaaaat ggagttttag acaactggga agatatttac agtgaaatgc
    16441 ctatagattt agaacctttg gaaagtaata ttacgcttac aaaccaaaat agaagttgta
    16501 ctgtatttac tagaggaata aaagagtatc aacatattga agatgaaatt gcattgacct
    16561 tacttgctac tacagatgaa ctaggaaagc ctgatttatt atatagacca ggacgtgctt
    16621 ctggtgatac aactaaaaaa ggtcatataa gaattaaaac agataaagct caaatgctta
    16681 aggaattaga attttcattt gcaatatata tggattctaa atcatttgat gaattggaaa
    16741 ttgcgaattt aacccacaat tatctaagag aatgtgtaaa ttatcaatta caggattaca
    16801 atttcttttt atatcgtata gataataaga ttcaaaaatc aattgtcaat aaaactgtaa
    16861 gtagagaatt agaaataata tctttaccag aaaactatct tatatcagca tgttatcctt
    16921 cttattacag taaagataaa tttataataa gagtggaaaa tccaagtagt aatagaatga
    16981 tattggatga gcaaatattt aaaaatagaa atgggaaaat tgtaaatgca atagaagatg
    17041 tagaagagga acaggtatat gaaatagcac cttttgacgt tatttcaata ttattggatt
    17101 tataagtgta aaagtaagtt tattgtttaa agtctgacag atattaatct gccagacttt
    17161 ttaatttgta taaatttggg taaatattaa tgaatggttt tagtatttaa gtatgaattt
    17221 agtatttaat atagaattta ttgaagtaga ataattagtt taacaggata agcagtttaa
    17281 a
//
LOCUS       DAMJYC010000087        16737 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJYC010000087 DAMJYC010000000
VERSION     DAMJYC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.089"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            81..755
                     /gene="fsa"
                     /locus_tag="PC472_003008"
     CDS             81..755
                     /gene="fsa"
                     /locus_tag="PC472_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2728317.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(877..2271)
                     /locus_tag="PC472_003009"
     CDS             complement(877..2271)
                     /locus_tag="PC472_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2728318.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2349..3695)
                     /locus_tag="PC472_003010"
     CDS             complement(2349..3695)
                     /locus_tag="PC472_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728319.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      3945..4047
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4141..5517
                     /locus_tag="PC472_003011"
     CDS             4141..5517
                     /locus_tag="PC472_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2728320.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(5614..6759)
                     /locus_tag="PC472_003012"
     CDS             complement(5614..6759)
                     /locus_tag="PC472_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2728321.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDINSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7012..7719
                     /locus_tag="PC472_003013"
     CDS             7012..7719
                     /locus_tag="PC472_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728322.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            7766..9361
                     /locus_tag="PC472_003014"
     CDS             7766..9361
                     /locus_tag="PC472_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728323.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            9464..10546
                     /locus_tag="PC472_003015"
     CDS             9464..10546
                     /locus_tag="PC472_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2728324.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            10695..10856
                     /locus_tag="PC472_003016"
     CDS             10695..10856
                     /locus_tag="PC472_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2728325.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGDRI
                     DKKKMNRPS"
     gene            11197..12465
                     /locus_tag="PC472_003017"
     CDS             11197..12465
                     /locus_tag="PC472_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2728326.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            12660..13448
                     /locus_tag="PC472_003018"
     CDS             12660..13448
                     /locus_tag="PC472_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2728327.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            13448..13897
                     /locus_tag="PC472_003019"
     CDS             13448..13897
                     /locus_tag="PC472_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2728328.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            13901..16165
                     /locus_tag="PC472_003020"
     CDS             13901..16165
                     /locus_tag="PC472_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2728329.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            16305..16616
                     /locus_tag="PC472_003021"
     CDS             16305..16616
                     /locus_tag="PC472_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728330.1"
                     /translation="MAGNLNNMRAVNNFRGDKSILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLSTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         6252 a         1936 c         2728 g         5821 t
ORIGIN      
        1 aaaacaataa gcaaaatgtt gaaaataata ccaaatcaat atataataag aataaagaaa
       61 gattaaaggg ggatatttta atgcaactaa tcttagatac aggtaatgtg gaggagatta
      121 aggagttatg tacttgttta ccaatagatg gtgtaactac aaatccaagt attgtttcaa
      181 aagaaaaaaa gaattttaag caacttataa atgaaattgg tgagattata ggtgaggata
      241 tgcctataca tgctcaagtg ttaagtacaa agtatgaaga aatattagaa gaagcattat
      301 atataagtag tttgagaaaa aatatatatg taaaaatacc tgtaacacaa gatggtttaa
      361 gagctataaa agaccttcac agaaaaggtg taaaaataac tgctactgca atattcactg
      421 ctcatcaagg ttttttagct gcaaaggctg gagcaaatta tattgcacct tatgtaaata
      481 ggcttgataa tatatctggt gatggagtag ctatggtttc agaacttata aaaataatag
      541 atacttataa aatggataca aaagtacttg cagccagttt taaaaatgca caacaagtta
      601 tagaattaat gcaacatggg gtgcatagtg ttacagtacc atatgatatt tgtaaatcta
      661 tgatgaatca tccattaact gattggagtg tagataagtt tatagaggat tgggagaata
      721 cttttggaaa aggaagtaaa acaaataata tttagtatta tggtaaaaga gcagttttgt
      781 ccaattttac aagaacttat ataaaaagat gaaaatatga attttctttt taataatttc
      841 gttattatct taagtcaatt catattttca tcttttttag atttctagtc ctataaattt
      901 ccaccaaggg aatgttatac atattattat aaagtaggtt aaaggaatca aagttaaacc
      961 gtatttgaaa ataaccttgc tattatacag attttcacca tgaccaacca ttattgtagc
     1021 atgatgaaat ggtaatatgt actgtatgct tactataatg tagacaaata gacataaagt
     1081 aacactattt tcctttagta tatgtaaact tccaagtaat ggtattacta cagatgatgt
     1141 agtgacagca cttccaagac atatattcaa gattattgtg agagttatca aaaacaatat
     1201 tgaaataatt tctgaagagt ttggtataaa ctttatcaaa taatttccca ttatatctac
     1261 aactccagag tactttaaca ctcctccaat tgaaaatgct gctataaaaa aaataaggac
     1321 cttccaattt atttctttta aagtactaaa tccaactatt tttcttagta ataaaagaag
     1381 tacacaaatt gacattataa ttaatgtgtt tatgttatgt atgaactgag ttaaaaacat
     1441 aaaaaacatt attcctaggc ataaaaatac gtgtttttgc tctttgttta gcaaattaaa
     1501 ttcttttttt aaattagaat atgaaaaatg aagtttatta ccctcaagat tttcattaga
     1561 aataaatttt attgaatgca attctttttt aaagacaaag ataaataaaa aataagttat
     1621 aaaacaagta attatagttg gaacagacat ataaaaagcc cattgacccc aatttatatt
     1681 acaatttcca agttctagta caacataatt tagaagagtg tctccattta caaaaaacat
     1741 tgatgtaaag gtagatgctg taaatatact aaataaaagt atagattttg aatcttcagt
     1801 tatctgttgt tcttttaaaa actccttgta aaaagcagat acaagaataa cccttggaaa
     1861 tggttgtggg ataaaaaata ttaaaagtat acctaaaata tacgataaaa gaatcatttt
     1921 tttaggggta tttacagtat ttaaaattaa cttctcagaa ataatttttg cgacacctgt
     1981 ttttgtaact gcattagtaa ttatgtatgc aagaattatt atgtaaaaat tttctgtaaa
     2041 taaaaactgt aaaataacct ttattggtgc aatgttaaat ataaacgcta gaattacaaa
     2101 aaacaatgca acaaaagttt tttcaactga gtctacagcc catatcaaga tagttaaaaa
     2161 agataagcat atagaaaatt ttatgttgaa actgtaatta agagagaagt agagaattat
     2221 ggtagctata acagtcatat aaaattgttt tggcttaaga ataataccca tttttatatt
     2281 cacctccata atttattata tcataatgga ggtgaataca atgaatgtga atacatttta
     2341 taaaaagatt attcattgat tctatgattt acaatagctt tagttttcca atgtcctttc
     2401 ttatagtaga taactgtaac aataagacct aaagtccaac caaaaggaat acccatccaa
     2461 attccgtctg ttccaaaaaa tttagaaagc atataagaag ctggaagtct tccaagccat
     2521 aaagatacta ttgagcttat aagtgcaaac atggaatcac cagcaccttt cattgcactt
     2581 gaaagtacaa aagaagttcc aataaataaa aagaatggtc ctattataaa taagtaagaa
     2641 gagccaattt taatgacatc tttatcagta ttgaatagag caattagatt ttccctaaat
     2701 agcaaaatta aagcaataac tgtaactgct aaaactaagg tcatttttat agattctcga
     2761 atgccttttt taactctttc attttctcca gcacctatgt tttgagcaac aaaagtagaa
     2821 acagcagaac caagattcat aataggtaag cttataaaat tttctatcct aagaccagca
     2881 ccaaaagctg ccatagcaga tgtaccataa ctattaacta aaaattgaag agccatattt
     2941 ccaattgaaa acaacatttg ttgaactcca cttggaagtc ctaatctaag tccatcttta
     3001 aatattttgt tatcatatac aattcctttg attttaaatt taagcacttc atgtttttta
     3061 tttaaataaa ttatacttat tataaatgat actgcctgag ccattatagt ggcaagtgca
     3121 acgccctcaa cacccattct tagcactaca ataaaagtta agtctaaaac tatatttaga
     3181 attgtggcta ttatcagaaa ataaagtgga gttactgaat ctccaagtcc tctaagtatt
     3241 gcacttactg aattatatcc agcagaaaat aatattccac taaatattat agtaagatac
     3301 aattttgatt gagcaaaaac ggattctggt gtattcataa gcttaagcat ggggccacca
     3361 aatactattc caagaatact tattaatata gaaccaataa ataaaagtat atatgtagta
     3421 tcaatagtag cttttacctt ttttaaatct cctgagccat aaaattgtga cactaaaata
     3481 gaaaatccaa ggctaacacc cataagcgct gccaccaata aaaacattat agggttagct
     3541 ccagagactg ccgccattgc ttcttttcca ataaatctac caactattat actgtcagct
     3601 gtattgtata gttgttgaaa taaactacca ataagcatag gcatggcaaa aaagaatatg
     3661 gactttccct catgacctgt agttaaatct ttcaccttat tcctccaaat aaaaaaatta
     3721 ttaatttatt atagtataat cttaacataa actttatatc caaactataa ataaattgta
     3781 tcaaaatatc atatattttt ttaaaatgta aagaatataa ttataatttg aatttaataa
     3841 atcaatattt aatataatcg aatattaata caaaatcaat acaatatata cgtatatatt
     3901 gacaaaatta attatataaa ttaaaataat attcgtaaag aaaaattcat gttttgtact
     3961 gtataacata taattttgac aatatgggtc ataagtttct accggaatac cgtaaatatt
     4021 ctgactatgt atactattat ttagttaatt aaatatttaa ttaactgaat tatgaaggat
     4081 acggctcagc ttatcaacta ctgtgtcgtt tttttgtaaa atttaatagg aggtgttgtt
     4141 atgcaaacaa caaacagtag tactatgcag aaactttttc caattttgac taataaaaat
     4201 gtcgacatga agaaggaaat aattgcagga gtaactactt ttttaactat ggcttatata
     4261 atagctgtaa atccaaatat tttatctgag acaggcatgc ctgcaggtgc acttgttaca
     4321 ggtacttgtt tagcagcagc atttggatgt attttaatgg gtgtagttgc taaccttcct
     4381 tttgctttag catcaggtat gggtcttaat gcattttttg catatacagt agttttacaa
     4441 atgggtgttc cttgggaagt agctcttaca gcagtatttg tagaaggtat tatttttata
     4501 gtattatcag taagtggagt tcgtgaggct gttgtaaatg cgataccaaa aaatatgaaa
     4561 ttagctgtta caggtggtat aggaatattt attgcactta taggtctagt taacagtgga
     4621 attgttattg gagaccaagc cactcttatc aaaatgggta gatttacacc agcagtaata
     4681 ataacttgtg taggattaat aattattgct gtattagata aaaaaagagt taaaggttct
     4741 atactttttg gaatagtagt gagttcatta cttgcttggg catttgcttt tataaatcca
     4801 gagtatgcac aaaaattagg tatatattta cctggtggat tatttaaatt cgaaagttta
     4861 gcaccaatag caggtaaaat agatttagga tatgtactac atccaacaaa tataggtggt
     4921 tttttagtag tagtttgtac atttttattt gttgattttt ttgatacagt aggtacatta
     4981 gttggtgttt gttctaaagc aaacatgtta gatgaaaaag ggaatgttcc taatgtaggt
     5041 agagccttac ttacagatgc ggttgcaact acagttggtg caggacttgg agtatctaca
     5101 gttacaactt atgttgagag ttcaacagga gttatcgcag gtggaagaac tggatggaca
     5161 gcaataacag ttggaatttt attcttagct gcaatgtttt tctctcctgt atttatagca
     5221 attccatcat gtgctacagc accagcttta atatatgttg gatatttaat gcttggtaca
     5281 gttaaggata tagagtttga taatataact gaaggtgttc cagcttttgt tactattgct
     5341 tgtatggcat taacatatag tataggtgat ggtttaactt taggtatatt aacttatgtt
     5401 ttcgtaaata tattttataa tatatttgga gctaaaaagg cagaagacaa aaaacatgtt
     5461 tctgtagtaa tgattgttct ggcaatatta tttataataa aactttggct actataatag
     5521 gtattattta aaattttaat ttaatataaa ataaagggct gtaatttatt atcaattgtg
     5581 atgataaact acagctcttt tgtatttgtt gtattattta ataatgtcta ttagttctcc
     5641 attttgtaaa gttttaggtg taattactat attgaactct ttttgcaaat cttttataat
     5701 atctaattct ttttttgtta ttattgagat tgtgttacca cttcgatttc ctctagcagt
     5761 tcgaccacat ctatgcaggt attcattttt actttttgga aaatccaagt taaaaacatg
     5821 acttacatcc acaatatcta gtccacgagc agataaatca gttgtaatta aaatctttgc
     5881 tttaccaagt ttaaatttat ttattgcatt ctttctatct tctttttcca tatttccaaa
     5941 aattcctatt gctttgtaat tatgataatt tagttttgat actaggactt ctatattttt
     6001 ttcattattt acaaatacaa tagctttttt tggatttgta gctgctaatg cctttctaag
     6061 aaaagtaaat ttatccctta tctcacctaa taaatatgag tgattgatgt taggatttat
     6121 ttgtgatttt tcctttgttt ttattatttc aaactccttc ataagtttat cacaaatttt
     6181 tatagtgcta tcagtaagac ttgcagagca tcctataatt tgtctatctc ttaacgttgt
     6241 tcttattatg tcttctatac aggttatatt ttttccattc aatagattgt ccacttcatc
     6301 taagacaata gtttttatat tatgtgattt tagtttcttt tgttttatca agtctaaaac
     6361 tcttccacaa gagccaatta ctatgtgtgg ttttactgct tttatatttt ttatttgttt
     6421 ttgtatattg acctccccaa ttaaagctaa agaagtaacg gagagttctg catttttagc
     6481 caataattca acctggtttg taatctgcat tactaattca tgagtaggtg caagtataag
     6541 agcttgtgtt tctctttttg atgtatcaat tttttcaaat ataggtaaaa ggtacgctag
     6601 tgtttttcct gttccagtct gtgaattaat caataaatcc ttattttgaa ttatatttcc
     6661 tataacaagt gactgaactt ctgttggaga atttatgtct tgttttttga gtccatctat
     6721 tagagtggaa cttattttta gttgttcaaa tgtattcata ttttgctcct tatattttaa
     6781 taattatagt caactatttt ttattataca ttgtttattc aaatcgaact atatattctt
     6841 ttataattat gtgtaatttg aaagtaaata tcaattatat ttataccttt atatttctga
     6901 tacctttgca ttttatagat ttctaaaata aacaaataaa agttgtaatt gtttgatatt
     6961 tgttatatat tatatataac aaatatcaaa actataatat ggagggaaat tatgttaaaa
     7021 gtaatcaatg tatctaaatc atataaaaat aaaaaagtag taagtagtgt ctcatttgat
     7081 gtaaatgatg gagagctttt tgggtttata ggacataatg gagcaggtaa aactactact
     7141 attaaagcaa ttgttggaat acacgatttt gaagagggag aggtactaat aaattctaaa
     7201 tctataaaaa aacagcctgt tgaatgtaaa aaggaaatgg catatatacc tgataatcca
     7261 gatttatacg agtcattaac tggtatacaa taccttaatt ttatagctga catatttgaa
     7321 gttgccaaag ataaaagaga agagttaata aaatattatt caaataagtt tgaactcaat
     7381 aaagcattag gagatttaat atcttcatat tcgcatggta tgaaacaaaa attagctata
     7441 atttcggctt tgatacacag cccaaaaatc ttaattcttg atgaaccttt tgtaggtctt
     7501 gacccaaaag cttcctttat attgaaggaa attatgaggg agttttgtga caaaggtggt
     7561 tgtatatttt tctcaacaca cgttttagaa gttgctgaaa aaatttgtga taagattgcc
     7621 ataataaaag atggaaatat aattgcttat ggaactacag aagaagttaa gggaagtaat
     7681 tcacttgaaa gtattttcat ggaattaata gagaaataaa atgtttttta tacaatatag
     7741 tgtctaaagt gaggaatgat aaaatatgag caatctaagt atattaataa aaactaatat
     7801 aataaatgaa tttaaattaa atgcgttaaa aaaagctgat ggtagagaaa aacaaaaaat
     7861 aatgggaatg atgctttcag ttgttataat agcgggtcta ttaatttttt atgtaacgag
     7921 cttaagtctc tctttagttg atatcttaaa gcaaataaat caactggaaa tgctactgat
     7981 atttggtttt ggattatcaa cattaacaac tattgttaca tcattatata aaacgtcatc
     8041 atatctattt caagcaaaag atttagacct tttgactagt ttacctataa aagaatctac
     8101 tgtactagca agtaagatat taatgttggt tggagcaaac tatttatttt cttcaatatg
     8161 tatgattata cctgcagtgg tttacttttt aaattctgat gtaagtataa cttatttccc
     8221 ttatttgata ttgctatttt tagctttacc attattacca atagttgctt catcaataat
     8281 ttttctattt atagggcaga tatcatcaag aataaaatac aaaaatttag tattaatcat
     8341 aggtagcatt atattgatgt tgctttatac cctacttatg tcaagactta attctatgtc
     8401 agttgaaatg ctaaagaata gtgtgtctat ttcagagaca attagaaaag tatatcctcc
     8461 tatatattat tttgttgatg ctcttaaaac aggaagtata gtttcatttt tagcatttat
     8521 agccatctca ataataccat tttttatgtt tgtaactctg tttgcaaaaa aatttaaaga
     8581 tataaatgct aagatgagtg aaggatataa gtcaaaaaat tatgaattta agggtcaaaa
     8641 aacttcaaga ccttcaaaag ctttattaaa aaaagaattt aagagatata cttcttcgtt
     8701 tatatacttt ttaaattcat ctgcaggttt gttgtttcta atagtaggta caattttaat
     8761 attattattt ggagcagata aagtgggtga gttattgaaa ttgaatatag attttaatct
     8821 tataaaaata caattattgg gagtattctt ctttataata gtaatgaatt gtacaacata
     8881 ctgttcaatt tctcttgaag gaaaaaactt atggatactg aaatcttctc caatagatga
     8941 aagagaaata ttctggtcaa aaatattgat gaatttttta ttgaatgcac cactatctat
     9001 tttatgtcta atattagtat ctataagatt agactttgaa attcagttta ttattgtatc
     9061 aatctgtttg attggaagtt tttcaatgtt tgtagcattg atgggattat tatcaaattt
     9121 attataccca aaccttgatt ggaaaaatga ggtggctgta gtaaaaagaa gtgcaagtat
     9181 gatagttaca atgttagtat cattagctta tgtaattata cttggaggta tatatgtatt
     9241 acttggaatt tcgtttttaa atatatatat tattttggca agtatactta ctttgatatt
     9301 agattttatg atatggagaa ttatttctac taaaggagta aaaatattta aaaatttata
     9361 agtgtattta tgtcaaataa agacttatct tttttagtct tgcaaataat aggttaagta
     9421 gtatactatg tatagtgata tgaaaaaatt taggagaaaa taaatgagaa aattatccaa
     9481 aagagaaaaa gattttattc aatatggatt tttattattt ttgatatgta ttactactta
     9541 tttagtatta actactttag atataaaact aatacctaaa attataaggc tagtaaataa
     9601 gacttatata tttcttggca tattgacaat tatagtatat atgataattg aatctattgt
     9661 aactcatttg ataataaatg cagttcaaaa aacaaaagta aaatttattg gtattaaaat
     9721 ggctacaatg ggattttact ataacttagt tactccattt gcatctggga gtcaaccaat
     9781 gcaaatatat gcacttacaa aatatgatgt aagccttagt aaatcagtgg ctatagttac
     9841 taataaaact gtagtatttc aaagtacagt tactgtattt tgtggaattt taattttgct
     9901 aaataagtca ttactgatgg agcaagtaca ttctgtgaaa gtcttgataa cttttggaat
     9961 gattatgaat gtaagtatgt tgttaggtgg actactaata gtatttagtc caaaacaagt
    10021 aaaaatgata gcagaagttt tgattaattt tttatctaaa tttaaaacat ttaaattttt
    10081 agattcaaaa cgtgatagaa taaatcatta tattgatgat tataattatt ctataaaaat
    10141 atttattaaa aatgtaagaa tcttagtttt aagtatatta cttactgtgt tacaattaat
    10201 cgcatatttc agtatagttt attgtgtgta taaagcctca aatttaagag gagtttcata
    10261 tgtacatata atgacattac aggtcttttt atatatggct atatcaccaa ttccaacacc
    10321 aggaaatgtt ggagctaatg aggttacatt ttttactatg ttctccaata taattccaaa
    10381 agaattattg gggtactcgg tactcttata tagtgggttt gtatactatt ttatattaat
    10441 agtttcaggt ttatttacat ttggaacgca ttatacatta aattcttgga aaaaggaaga
    10501 taaagtaaat tctaatttag ataaggttat tgaaaaatta aattaataaa ataattaggg
    10561 aaattctata tataaatatg gtgttgagta agctatatta tattgagata tttccctttt
    10621 ttgattaaaa ttatagaaat ttgaaataat aagtaataaa cagaaagagt gatattgttt
    10681 tgaaaggaga aactatgaaa caagataaaa aaaatgaaaa acaaagtgag atttctaaga
    10741 taaaacaaag caatgataat aaacttgaaa tagctcaaga attacatgta agtaaaaata
    10801 tagggaatat gcttggagat agaatagata agaaaaaaat gaatagacca agttaatgaa
    10861 aatgttgaaa atggtacatt ttaaaaaata tatataatga aatgttttat tgttaaattt
    10921 aaacttattt taatggagga aaaagtcgaa aaatatctaa aaagatatgg atataaaaaa
    10981 tattaatata tatatactat aaacttgaca aaatcaacct tttaataact agcaaaaaat
    11041 agcaaaatta tataagcgtt atttaattgt caaaaatgaa agctaaattg catgtcctaa
    11101 aaaaaatgat tgataacatt tatttgtgtg ctataattat atagtaaaac aataagtata
    11161 ttgcataatg tatgcaaatc gggaggagag acggacatgg aagcatctat acacacaatt
    11221 gccagcaata atttattgat aatttttgct attgtaagta taactggaat aatttgtagt
    11281 aagcttagtg aaataataaa ggttccagat gtagtgttat atttacttgt aggtatttta
    11341 ataggaccat catttttaaa atttatagat attcgtggat ttcaaataga aaatcaacta
    11401 atacttacat ttggttctgc atttatttta tatttaggtg gaaaagaaat aagtttaaaa
    11461 gtattgagaa atgtgaagat atctgtattc ttattagcta ctttaggagt tgtgatatct
    11521 gcttttataa tgcaacaagt aataggattt acttttggga taagtgcaat gactgcttta
    11581 ctagcaggaa ctataatagc atcaacagac ccagccactc ttgtacctat atttaatcag
    11641 gtaaagataa aggatagggt aaaacaaact gtaataagtg aatcagcttt taatgatgca
    11701 acaggagcta tactaacatc agcagtgttg gctgtaattt tgtcaaataa attttcatta
    11761 ggtgaaaata tttatgaact ggggacaatg gttattgtcg gagttttagt tggtttaatt
    11821 acaggaatac tgcttttgaa attagttaat gataaacctt atggaatatt taaagatttt
    11881 gcaccaataa tatctgttat ttcagttgta gttgcatatg aattatctac aaaattaggt
    11941 ggaagtggat atatgtcttg ctttattgta ggtataataa ctggtaacaa gaaaaacttt
    12001 aaaatttggt taagtcaaaa atcttatgat gcagattttt atgttgctga aactttaggt
    12061 actatttgtc gtatggcaat atttataata ttgggtagcc aagttaattt ggctcagtta
    12121 agtaaatatt ttatgccatc tgtaattaca gtattagtat taatgtttat agcaagacca
    12181 ctttgcgttt tagtttgtac acttgtagac agggaagcaa aatggagtaa aagtgaaata
    12241 ttatttatga tgtgggtaag ggaaacaggc gtaataccag cagcactttg tggtattatt
    12301 tcagcaatga aagttcctgg atacgaagtg atttcatctg tagtattcat gactatacta
    12361 attacattag taatacaagg aagtactaca aaattagtag ctaaaaaact tggattatta
    12421 gaagaagaag tgataaatat aagtgaaaaa gtatcaactt tttaaagttt tgctttaaaa
    12481 tagtacctgt taatggctat tttaatattg tctaatgatg attattgaaa tagtcatttt
    12541 tttatgtata agtaatttac agaatttaca tattagacat atttattatc aaaatttgtt
    12601 gaagctatga tataattagt tgtatttaaa ataagaatag caaataaggg aggaaattta
    12661 tgtttacggt tatggatatt gtacagccag atacagtaga agaagcgtac tcaattttaa
    12721 ataaaagaaa aactaaccaa gtaattggtg gaagtgcttt tttaagaatg gggaaaaaaa
    12781 gaataggaac aggaatagaa ttatctaaat taaatttaga ttatataaaa gagtatgaag
    12841 attacgttga gattggttct atgactactt ttagaacatt agaaacaagt tccataataa
    12901 aaaataattt tggaagaata atagaagatt cagttaaaaa tataatagga gttcagttta
    12961 gaaatgtagt aacagttggt gcaacagtat tttctaaata tggtttttct gacttgatag
    13021 tagcattact ttcattagat acggaagtag aattatataa tattgggaga attagtcttg
    13081 aagagtttct aaatagagat tatgagaaag atttacttat taagatatac ataaagaaga
    13141 caaataagaa tgcttcttat aaatcaatta gaaatgcaaa gagtgattat ccaattctaa
    13201 atgtatctgt atcaaaacat atggaacaat ttaagttatg tgtaggagca agaccgcaga
    13261 aagctacgat tgcaaaacaa gctagtgaat ttctatctaa caatgaaatc aatgaaatta
    13321 atatagataa agcagtagaa atagcatcag aagaactaac ttttggttct aacatgagag
    13381 catctaggga gtatagaaaa gctatgagta aagtactttt aaaaagagct ataatggagg
    13441 ttatttaatg ttagtaaaga ttaatataaa tgggaagaaa aaaagtgttg atatagaacc
    13501 agaagaatat ctggttgata ctttaagaaa agttggtaat ttaagtgtga aaagaggttg
    13561 tgacacaggt tgttgtggtt tatgtacagt tttaattgac aagaaaccta cattatcttg
    13621 tgcaacactg acattgagag ctttagataa agaaataact acaattgaag gtttagaaaa
    13681 tgaagttaaa gaatttagtg aagttttggt aaaagaagga gctgagcaat gtggtttctg
    13741 ttctcctggc tttatactta ctgtacttgc aatgaaggat gagcttgaaa atcctacaga
    13801 tgatgaaata aagcattatc ttacaggaaa tctatgtaga tgtacagggt atatgggaca
    13861 gttaagagct atcaaaactt atctggaggt aaaatagaac atgaatatag ttggaaaagg
    13921 aatcaagaaa atagatggaa tgagtatcac aacaggaaag cctgtatata cagatgattt
    13981 atcagcaaaa gatgctttgg tggtaaagat acttagaagt cctcacgctt atgcaaaaat
    14041 aaaagatata gatacatcaa aggcagaaat ggttgatggt gttgaatgtg ttttaacata
    14101 taaagatgta ccaaaatctc gatttacact tgctggtcaa acatatccag agccatctcc
    14161 atatgataga ttaattcttg aagatgttgt aagatatgta ggtgacgaag tagctatagt
    14221 ggcagctata gatgaaaaaa ctgcaattaa agcaatgaat cttatcaaag taaagtatga
    14281 agtacttaca cctgttttgg attttgaaga ggcaatagga aataaaacta tggtacatga
    14341 tgaagataga cattgtaact ttgatatagg tatgcaaaga gaaaaaaata tagtatccca
    14401 tcatgattat acaaaaggtg atgtagaaaa agtattaagt aattgtgatg tagtgattga
    14461 agaaacttat tatactcaag ctcaagcaca tgcaatgatg gagacgtata gggcatacaa
    14521 ttatttagac catatgggaa gattggttgt agtaagttca actcaaatac catttcatgt
    14581 aaaaagacat ttatctcgtg cactagaaat accagctagc aaaattaggg ttataaaacc
    14641 tagaattggt ggcggatttg gtggtaaaca aactgcatgt gttgagattt tttctgcaat
    14701 tgttacttta aaaacaggga aaccagctaa aataatttat gatagaaaag aaacttttaa
    14761 ttgttcaaca agtagacatg caatgagatt aaaagttaga ctaggtgcta caaatgatgg
    14821 cataataaag gcaatagata taaaagcact ttctgataca ggagcatatg gagagcatgc
    14881 ttctacaact attggtttgg ttggagaaaa aactttacca atgtataata aagttgaagc
    14941 aacaagattt attgcagatg tggtatatac taataagatg ccagcaggag cacttagagg
    15001 gtatggagca tcacaaggat gttttgctgt tgaatcaact ataaatatgc ttgctaataa
    15061 attaaaaatg gacccaacag atttaaggtt aaaaaatgtg gtaacagaag gtgaaactac
    15121 atttgcatat ggtaaaactt taaatagtgt cgatttaata aaatgtataa ataaaggcaa
    15181 agaacttatt ggatggaatg aaaaatatcc ttgtaaacat cttgataatg gaaaaattag
    15241 aagtgtgggt atggcaatta ctatgcaagg ttctggtata caaggtcttg ataagtcttc
    15301 agctgaaata aaattgaatg actgtggaga ttatactctt atgataggtt ctactgatat
    15361 gggtacagga agtgatacga ttttagctca aatggctgct gaggttcttg atacaagcat
    15421 tgaaaatata acagttatat cagctgatac tgacttagtt ccatatgacc caggttcata
    15481 tgcttcaagt actacttatg taacaggtat ggcagtagtt aaggcagctc aagacttaag
    15541 agaaaaaata atttctgctg gagcaactaa actaggtgtt gagaaggatg aagtagaatt
    15601 tgatggtata aaattaacta gtaaaaaagg tagcacttta ttattcgata tagcagttga
    15661 ttctacaatt ggtccagata ctaatcaatt aataggtctt gcttctcatg gtagcccagt
    15721 atctcctcca ccttttatag caggttttgc agaaactgaa attgacacag aaactggaaa
    15781 agttgattta gtaaattatg tagctgtagt agattgtgga acggttataa ataaaaatct
    15841 agctaagata caagttgaag gtggaattgt tcaagggatt ggaatggcat tatttgaaga
    15901 agttagatac acagaaaaag gaagcatgga cacaaataca tttatgcaat acaagattcc
    15961 agctagatgt gatattggaa atgttaaagt agattttgtt gaaacttatg aaccaactgg
    16021 accatttggt gctaaatctg ttggagaagt tgttataaat acacctttac cagcaataca
    16081 agaagctgtg tttaatggaa ttggtgtaag aataaataca ctccctatta cacctgaaaa
    16141 agtttttatg gaaatgaata aataattgtt tatgaaaatt tagactagtt ttacatgtta
    16201 aacaaccagt tatgtcatac ttgttgaaag atatttaata agtagttatt ataatattat
    16261 gaattttact tataaaatac aaaattaaat aaggggatga tgttttggct ggtaatctaa
    16321 ataatatgag agcagtaaat aattttagag gagataagag catcttagaa tgtttagtca
    16381 gctttgaggg tcgttcaata agtcagagaa aagtaagggt attttttaaa gaaaaacaaa
    16441 atcaaataga aattgatttt gcagaagagg aaatttctaa attggttgaa aatgttgttt
    16501 taagtacatc atatcaagaa atgttatatg atgaaataga gaaacaactg gaaattgatt
    16561 gtataggtac ttggatgata ttatctaaat taaaagatgg tagtagagtt cactgatttt
    16621 aaaataaatt attagagcct aattttaagg ctcttttttg ctgtttttaa ttaacaatag
    16681 aaaatttatc tttagtttgt atataatttt aatatcatat attctatata tataaaa
//
LOCUS       DAMJYC010000088        16320 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJYC010000088 DAMJYC010000000
VERSION     DAMJYC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.090"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(141..962)
                     /locus_tag="PC472_003022"
     CDS             complement(141..962)
                     /locus_tag="PC472_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2728331.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(1153..1506)
                     /locus_tag="PC472_003023"
     CDS             complement(1153..1506)
                     /locus_tag="PC472_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728332.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(1548..2990)
                     /locus_tag="PC472_003024"
     CDS             complement(1548..2990)
                     /locus_tag="PC472_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2728333.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            3666..4877
                     /locus_tag="PC472_003025"
     CDS             3666..4877
                     /locus_tag="PC472_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2728334.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            4969..5484
                     /locus_tag="PC472_003026"
     CDS             4969..5484
                     /locus_tag="PC472_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2728335.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(5751..7850)
                     /locus_tag="PC472_003027"
     CDS             complement(5751..7850)
                     /locus_tag="PC472_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2728336.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7974..8513)
                     /locus_tag="PC472_003028"
     CDS             complement(7974..8513)
                     /locus_tag="PC472_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2728337.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            complement(8516..9256)
                     /locus_tag="PC472_003029"
     CDS             complement(8516..9256)
                     /locus_tag="PC472_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2728338.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            complement(9258..10307)
                     /gene="vorB"
                     /locus_tag="PC472_003030"
     CDS             complement(9258..10307)
                     /gene="vorB"
                     /locus_tag="PC472_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2728339.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            complement(10324..10560)
                     /locus_tag="PC472_003031"
     CDS             complement(10324..10560)
                     /locus_tag="PC472_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2728340.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            complement(10575..11678)
                     /locus_tag="PC472_003032"
     CDS             complement(10575..11678)
                     /locus_tag="PC472_003032"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2728341.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            complement(11781..13211)
                     /locus_tag="PC472_003033"
     CDS             complement(11781..13211)
                     /locus_tag="PC472_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2728342.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            14000..14446
                     /locus_tag="PC472_003034"
     CDS             14000..14446
                     /locus_tag="PC472_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2728343.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            14448..15542
                     /locus_tag="PC472_003035"
     CDS             14448..15542
                     /locus_tag="PC472_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2728344.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLFSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            15630..16160
                     /locus_tag="PC472_003036"
     CDS             15630..16160
                     /locus_tag="PC472_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2728345.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT         5514 a         2708 c         2029 g         6069 t
ORIGIN      
        1 atttaaaata agctatacta tttaaattta attctattta gttattttag caaaataaaa
       61 acctctagta tttttctaga ggatacaata ctataactat taacttttta actatctatt
      121 aatttacttc aaaacacact ctaagctata ttgttagatt taactttttc tatatttaca
      181 aaataggttt ctctttcttt aaataaagat acatatttaa agtcaagata cttaagcata
      241 tcataggcca catcaaaatc tttgcatata tcttctaccc tatgagaatc agaacctaca
      301 gtaactataa atcctccaag ttctttatat ctttttagaa tatcttcatt tggatgtata
      361 gcatttagtc catatctata accagaagta tttagctcta taccactccc attattgatt
      421 attgacttta agacctcatc aataacatct ttaaagtcat tgtatataac tctattatct
      481 gaatatattc catacctaga tatataatcc aaatgaccat atatattgta atctccaaat
      541 tcacgattag tttcaagtat gctattaaaa tatttataat acgcatcttc tttgctaaga
      601 cctttaaaat acgcctttga tggaacaggt acattgtcaa ttgtatgagt tgagcacaat
      661 acaaaatcaa aaggattaga atttattatt tcttctattt cattttttag atgttttctg
      721 taacccactt caacacctag taataactct atgtcctttt tatatttttc tttaaactta
      781 ttaaatactg gaacatagtc attataattt atgcaaaatc ctctatcttc atcatgttct
      841 aaatggtctg taaaagtcaa atatctaaca ccaatactta tagctttttt tattgtatct
      901 tccatggaat cctttccatc acttgaaaaa ttagtatgaa tatgattatc tattaaattc
      961 atattatccc tccctttatt ttatctatct taattataca acaagtttat tgtatacatt
     1021 tgttaaaaga aaattataat ggttaaattt tatttacata cttttatttt tacaaaaaag
     1081 agaagcacct atttctgatt ttagaaaatc attaaggata cttctcctaa tgttactttt
     1141 atatacttca atttaatttt taaaagtctt aattttgtaa tagagtttag ctgttatatg
     1201 tccaatcata taaaaagctg tataaagtaa aacatatata tatgcagaat catttaaaaa
     1261 tatatacatt aatactatat aagttactaa agaaacaata agagataaaa tccaattagt
     1321 cttatttaat acacaaatta tcccttgtat caaaaaagaa attggaaaaa tcaaaaccat
     1381 tcccacaaca aaaatacttt tagattgaat atctggaata ttaaatatat ctgtagcaaa
     1441 agttaaaaac attagcacca ttatgactgt aggaaatatg atactaaaaa tattactctt
     1501 tttcataata atcaatcctt atttattgga tatttgataa tcttcaatta ttttgttgcc
     1561 tcaatttctc ccatcattct tactaaatct tgtccatatt gacctccagc ccaattatta
     1621 gataaacttt caactgtttt agccttacca aataaagatt tttcatgtct agggtctact
     1681 ggatttgaaa gtgggtaacc atcttgtcct gcatataagc ataaatgttg aatttgagct
     1741 aatattcctt cttcccaagt atcaaatctt gaatgggcat tcttatcagt atcagaacca
     1801 cctacagaag ttttaagtcc acaaggattc ttaaatgatg catctaatac tccaccaaaa
     1861 ttacaaaaac cagtttcttt tgcagattga gctactgcca aaacaggatt aacaccttcc
     1921 tgaacagcag tatcatataa tattggtatt aactctataa ataaatcatt agctttttta
     1981 gattttgccc attttaagca cgcttctaaa ctagctgttg gctgacctaa tatgcttgtc
     2041 tcatttttca taggtgactc accttttgta gacgaatcgc cattattttg tgcagcagaa
     2101 acacacttat tatacacact gtcacttatt gaacctaatc tagtaatctt atctgctcct
     2161 gctaatatag acttatcact actttctgac accaatacaa ttggattttc cttagcaatt
     2221 gtagaacctg taagtgcatt tactaaatca tatccatcac tcaaataaaa ttctgatgca
     2281 tctggataaa actcttttat aactttctta tttgtatcat atctatctga accaccaagt
     2341 cttttagcat tagttttatt aactaaacta gtgctcattg atatactccc acctactgca
     2401 tatgttttta cattgcttgt tgaaaaaggc acactctttc catctgtaag tactatagga
     2461 gctatatctc ttactgatac gggtgcagca cttattgtat ctgcttctcc tttatatgca
     2521 ttagttaata tcacttcatc aacttttttg ataccattta cctctttagc aacactataa
     2581 ctagtcttta ttctgtcatc tccacccaat ctagttacct gtgcccctgt gttttttagt
     2641 tcagtttcta ctgacttact tattgataat tctttaccta ttatgtatat ctttttaaca
     2701 ttttttaatc tttgttttgt ttcatttggt atcttatttt tttgtgcaag caaaattgga
     2761 gcatcaacag cacctgcaag accacttgct gaaagaccat ctgctataga attatccata
     2821 tttaccataa ttgctgtatc ataactcttt ttatctgcta ttaatccagc cgtctcatat
     2881 atattgttac ctttaattgt atctgatgtt aatgcatcta cattaggtac atttattaga
     2941 aatatagcta atgttaaacc taatgctaaa actcttctaa ttgttctcat attcttcccc
     3001 cttgatatat caagtttaat tcaaaaatga ctataacata gtattacatc tacgctatag
     3061 tcatttatta taatttaata attatatatt tgttattata tcataaatta caaaaagttt
     3121 ccataatatt gtatagatta aaggaaaaat ttacatttta gttacaattt actagatttt
     3181 tgttctaatg tacctatatt tagtaacaat ttgtaataaa aatcgatatc tatattacat
     3241 atttaattca ctgttttaca aaatgaaatt ttattgtata caataaatag attatatgtt
     3301 tacataatct atattatttt taagtaaatc ttttatcgat attattaaga atatatatta
     3361 catcaagttt attacgtgca aaccactatc aacttaaata cctaaggtaa cacttgaagt
     3421 agtagattct tggtaacaat aattattgct atcattatct ttttttaatt aagtatttac
     3481 tatttatata tcaatctact tttcactttt aatttaattc taatattttt ttatatgttt
     3541 tatgatatat aatgtgatat attctaatta atgatatttt caagtaaaaa aatgttactc
     3601 tattataagg tgatagtata cttgaaaata aataaaacac ttaactttat ttaaggagat
     3661 ttatgatgag taacaaacaa ggttctaaca tcaaagatgt aaaaattaaa aataagttaa
     3721 taatattgaa attactttct acaaatacac ctatgtccag agttgattta gctaagtcta
     3781 caggacttac taaaatgact ttatcaaatc tagttactga acttattaat gaaaatatga
     3841 ttagggaaat agagtcatca actttaaatg cacattcatc aggtcgtaaa cctattttat
     3901 tagatatttc taatatatcg ccttgtattt gtggaatcct aataagaaga aatatatgta
     3961 aggtaattat ttcagattta aaaggtaata ttgtaaaaca actttctgaa acttacccta
     4021 aatttctatc aaatgatgtt ttaaaagata taataaaaag aatgtttact aaaataatga
     4081 aaatcaacaa gagagatata attggaattg gtatttcttc aataggtcca gtagatgata
     4141 taaatggtac catactaaat cccccagatt ttggaaatgt atcaaaccta aatatagtag
     4201 attttataaa agaaatgtct gatttgccaa tatttttgat aaatgatgct aattcgggtg
     4261 cattagcaga aaaaatgtat ggtttaggta aaaatatatc aaattatata tacctacaca
     4321 taatgaatgg tattggagct ggtctagttc tagaaaataa acttcatact ggcaacctag
     4381 gacaaagtgg agaaatagga catacatcaa ttaattttag tggaccattg tgcagttgtg
     4441 gtaacaatgg atgtttagat tattatacaa gtgtcagtaa tctaattaaa agaattgaat
     4501 cattgagcca tatttatcct aactccccat taattaactg taaagatttt tctttagtca
     4561 agttaataga tgaggcaaat aataaagata gtcttgccat gtttttgctt gatgaatttt
     4621 gtacatacgt atcatatgca ttagttaata cacttacatt gattgattgt agttctataa
     4681 taataggcta tgactttaat atacctggta catttataga aaatacatta ttaaaaaaat
     4741 tgactgcttc agcaagtttt tctaaatata aaaaaataag tgtaaggcat tcaaattttg
     4801 gagcaaatgc accattaatt ggctctatag ccattgtaag ttataatttt tttaatggta
     4861 gtataaattt ttattaaata tgtaaaatat ttaaacactt ttattgaata tttttccatt
     4921 taatattatt atattttata agagcattta aaatttggag gtataaaaat gatttcagct
     4981 aaaatggaaa aattacttaa tgagcagatt aaccatgaat tatattctgc gtatctttat
     5041 ctttctatgt cttcttacct tgaagcagaa ggacttaaag gattttctaa ttggttttat
     5101 gttcaatata aagaagagac agaccacgct atgttttttt acaaatactt acataatgta
     5161 ggaggaaagg ttcagcttga tgctatccca atgccagatt cagactttac ttctgcaatg
     5221 gatattcttg agcgtacgtt agctcatgaa aaaaaggtta cagctttaat aaacaactta
     5281 gcagctgtag caaactctga atcagacttt agaacttctc aattcttact ttggtttata
     5341 tctgaacaag cagaagaaga aacaaattgt gaagacaata taaaacgtgt taaacttgct
     5401 ggagagggtg ggctattctt tgttgaccaa gaatttgcta atcgtacata tgcagctcca
     5461 acaaacccac ctgtgacaat ataacgttta aaaattttta atataataaa gaaggtgttt
     5521 taatgaaaat caagattgat tcttatcatt aaaacacctt ttattattcg ttttatacgt
     5581 agtattttat tgtttataca gtgctatttc acaatgaaac ttaaaactat actttcattt
     5641 taaaatattt ttaaattaaa tttggggtga ctgaaaatca cactatctaa cttacaaaat
     5701 gtgattttca gttggtgctt taattatggt tgaataccaa aacttataaa ctatttatat
     5761 ttaactatta aggcatctac tgcacatacg ccttttcctt taggatatac aaacactgga
     5821 tttaagtcta tttccttgac ttcatcctta ttttcataag catattttcc taacttgacc
     5881 atcatatctg caagagcatc aatatcacat ggctcacttc ctctatagcc atttaaaagc
     5941 ttaaatgatt ttagtttttt aagcatcata atagcttcat catgatttat aggtaatgga
     6001 tataatgtag tatctttaaa tacctctaca aatactcctc ctaaaccgac taataacatt
     6061 ggtccaaatt ggtcatcatt agttatacca ataataattt ctataccact ttctaccatt
     6121 tcttgaacta atataccatc aatattagca tctggttttg ctttttttac attagttaaa
     6181 atctcattat atgcatctac tgcctcatct ctattcttaa ttcctatttt aactcctcca
     6241 acatcagatt tgtgcaatat ttcagaagaa tttattttta aaacaacagg atacttaata
     6301 tattttaaga tttcatctaa ttcatcaata ctttttgcat taccttgtcc tggaactggt
     6361 actccatatt ttttcatctc aatcttacta tctaattctg ataatgctac tacattattt
     6421 gactctcttt tcttaacagc tttaaattct aaagttcttt ttgaataatc ataatctata
     6481 aacttagcca ttttattaaa acaagtaaat gtagttccaa ctgatgacat tataggcact
     6541 tgattatctt ctaatctagt cctataatct aaatatctat ttccttcaag tgtaggaact
     6601 gcaaacacag gttttttcaa tccttgttct ctagcttcac ttacagcttt acaaagggtt
     6661 tcatgcattt cattttcttc atctcttata tttgcaccta ccatagtaaa tccaacacta
     6721 tcatcctttt caaaggtatg taataaacct actatcatat ccttttcatg gaataatgct
     6781 gttgtagcat ctagtggatt tttaggtgtt gcaaatcctg gaagatattt ttccatttct
     6841 tctttagttt cagaagatat ttctgcaagt tctacaccat tttcttctgc taaatctgca
     6901 caaatagtat tttctcctcc tgagaagttt attgctgcaa aattagaatt tgcaggtaaa
     6961 ttaccatcta aaacactaaa agcttgtgct aaacacataa actgttctaa attttccaca
     7021 gaaattactc catatttttc aaaaatactt tcaaaagctt tactcgaacc agctaaattt
     7081 cctgtatgag aagcagcaga aatagcacct ttttctgacc ttcctgattt caatattatt
     7141 actggctttc ttttttgtgc tgcccttttt aaagcatcta taaatttttg aggatttttt
     7201 aaaccttcaa ggtaaattgc tattactgat gcataatcat catctacaag gaaatctact
     7261 gcatcttcta gtgtaactat atttccattt cctgttgaaa aaccataaga tatattaaaa
     7321 taatcacttg aaacaatttc agcagttata aatccacttt gacctacaat tcctatacca
     7381 ggttttttag tatctaaatc ccatttagaa cctcctgccc atagattaac cttatctata
     7441 caatttagca accccatgca gtttggaccc aatattttca tatcgtatgt atttgatatt
     7501 tctataagtt gattttctaa atctgtacct tcctctgtac cttcctctgc aaatccacta
     7561 gcatatacca ctgctgcctt tgttcctaac tctccagaat cttttaatac agatggaact
     7621 acatttctag gcgtacatac tactacacag tctactactt ctggaacttc ttgtatcgtc
     7681 ttataacatt ttctcccaaa caattcttct cttttgggat taacataata tactctatct
     7741 gtttccttac tttttactat actaagtgct gcacttcttc caaaaccaag tttatcagtc
     7801 actcctacta tagcaatact ttttggtcta ataagttttt ccaaactcat aaattcacgc
     7861 tcctatatgt ttatagttgt gattttaagt gaattttatc tcaagatgaa gtttctcttg
     7921 gcactaaaga ttattaaatc ttgagataaa tcacttaaga taagggtaat ttatcaaatg
     7981 gcgttattat agccttccat gaatgctgct tcattttgaa cattaaactt tccttttcct
     8041 ttttcctcaa agaaatcaca catagttttt aaagctaatt ctttttcaaa taacccagtt
     8101 ttagcaatta atgctccaag tactactata tttgctgctt ttggattgtt tactttttga
     8161 gctaattcct gacaatttat tttaactaca cttatatctt ctcttaatgt tacattctca
     8221 tctattgcag atgtattaat aactactgtt ccaccttctt taactaaatt tatataatct
     8281 aaagatggtt tattcattgc tactaaaaca tctatttctt ccatatcagg cccacctata
     8341 ctctcatcag aaaactttac tacactatag gcttttccac ctctcatagt tggaccatat
     8401 gcaggcatcc aagttacttg tttccccaat tcaagtgctg actcagctac aatcagacca
     8461 agagttaaaa ctccttgacc accaaaccct gcaaatacta gctctttttc cataactatt
     8521 caccatccct ttgcttaaat tcgccaagag cataatatgg cactatttct tcttccatcc
     8581 atttgataga tttctctagt gatacacccc agttagttgg gcaaggtgct aatatttcaa
     8641 ctaaggaata tccctctcca tttaattgag cttctattgc attttttata tattttttta
     8701 gattaattat ttcctttggg ctatgaactg aacctcttgc tacatatgct acatcaaaga
     8761 agtttgccac catctcagga actttcagtg gactaccagt agtctttata tctcttccat
     8821 ttacacttgt tgttgtgact tgacccggca tagtggtcca tgacatttgt ccaccagtca
     8881 ttgcaaaatt attattgttt attacaaata cagtcacatt atgatttcta tatgctgtat
     8941 ttaaagtttc agaaagacct ataacataag catctccatc accttggtaa gtcattacta
     9001 atgtatctgg aagtgctact ttaactccta tagctgtaga tgaagctcta ccatgtgcag
     9061 tttgcatctt atcaccattc cagctaaaat tcatattgca gacacaacca actcctaatg
     9121 ttaatacatg atttttttca tatccatgtt cttctattac ctctgctatt atacgattta
     9181 ctattccatg accacaacct gggcaaaact tgtttggtat tgaaacaacg ctaggtgtta
     9241 atttttctgt agccatatta gaacacctcc ttgatttcgc cagattttat tttgctataa
     9301 gtgtccatta cttgcaatgt ttttggtata tgatttccac taaataaacc atacacaggt
     9361 acattttctt tacatacttt ctttgaggct aaagcaacat cttctactaa ttgacctgtg
     9421 tctgttgatt ctactgagat aaatcctttt acatctttac ttaccttttc aaatgcttta
     9481 tatgggaaag gccatactgt tattggtctt attattccaa ctttttcacc ttgttctcta
     9541 agtcttctaa cagcattcat agttgttctt gatggtatac caaacgctac aaacacatat
     9601 tctgcatctt ctacttgata atcttcccat tgttgttcat tttcagttat tctcatacgt
     9661 ttctccatac aaaaagttgg cttttggtta tctgcaacct ttgcatggtc tctattactt
     9721 ccatcataag tccaacctaa atctccttct ctcttcttaa actctggaaa ttctactggt
     9781 tccatcattt gacctaaagc tgcttctgtc aaaatttcga caggcactct gtacttctcc
     9841 gctacatcaa atgagttata catcaaatct acagtctctt gaatactgtt tggtgcataa
     9901 actatcaaat gataatctcc atttcctccc cctctagtat ctcttagata gtctgtctgt
     9961 gctgagtcca atgagccaag accaggcccc catctctgta catttacgat tacacaaggg
    10021 taatgatttg cacataagta agaaatccct tcttgtttta aagatattcc tggtcctgaa
    10081 gaactcgtca tagacctcat tccacttgca tatgctccat ataccatata tatagcagcc
    10141 atctcatttt cggactgtat aaaagttctt ccaagttcag acattctatg tgataaaaat
    10201 tccattacct ctgtttgtgg tgtaattgga tatcctgcat aaaattcaca acctcctctt
    10261 agggctgctt ctgcaatggc gtggttcccc ttccacatct ttttcatatt atcccaactc
    10321 cttctaagcc tcttgacatg cttcttcttt aattatttca aaaacattat ctggacacac
    10381 ctgatagcaa attccacaaa gtatgcattt actctcatca acttctacat gagcataacc
    10441 ttctttattt atcttacttg atatttttaa tgctcctttt ttacaagata taacgcagta
    10501 ttcacaactt ttacatctct ctgcgtttat ttttaagttc atatctacac cccgtttcat
    10561 aatatttcta tctattatcc aatacttggc tctcctttaa tatttaatat ttcctttaaa
    10621 actgctacaa atttcctatt ttcttccata gttccaatac ttacacgtgc atgtgtagca
    10681 cttggtctta ttacaaaccc tctttttttc aagtcttcaa ataacttagg tatatctgtt
    10741 ttcatatcta cataaaggaa atttgattgt gaatctacaa catcaaatcc catttttgta
    10801 aactcttctt taaagtaagc cattccaact ctatttaatt catatgtttc ttttataaac
    10861 tcttcatcat caagtgctgc tgttgctcca cttatagcaa cacgatttgc agagaaaggc
    10921 tcttttactg taaataaagt tcttattatc tcatctttag caataccata tcctactcta
    10981 agccctgcaa gaccatatat tttagagaaa gttcttgtta ctataacgtt ataattttcc
    11041 cttacatact taagtccatc cacataatta ggaactgtta aaaattctat atatgcttca
    11101 tccaatatag ttataatatt ttcagggact ttctttaaaa attcttctaa ctctttatca
    11161 tccacaattg ttcctgttgg attattaggg ttacatacac atattaattt tgttttatct
    11221 gtaatcttat ctaatatacc ttgtaaatca tacttataat cttctgttaa aggtacttct
    11281 actggtatcc cttcattttt tatagttgca gttctatatg atggaaatgt tgggttacaa
    11341 tatataactt catctcctct atttataaaa gcctcaccta ttagagtgat aacgttatct
    11401 gcaccattag caacaataaa attttctggt tttaaatcta gcttttcagc taattttctt
    11461 ctaagttcgt tagcttctgg ctctggataa agttgacctt gttttaacat ttcaatcata
    11521 gcttctattg cttttggtga tggtcctaga ggattttcat ttgaagctaa ttttattatt
    11581 tcttttacac catatttttc cttaactttt tctattggtt cacctggaac atattgatgt
    11641 aattctttta cggtatttct caataatttt tctatcattt ttaaaagccc cctctgtata
    11701 tgtattttca tttatataaa cgctattgcg tctacttact ttaatagctt aaattaacca
    11761 tctactcaac tttcttttaa ctaagcattt ctttgattta actcttctct tatttggttt
    11821 aatttcttgt cacttactgg atatagtatt gctataacaa cagctattat taaaggtatt
    11881 gccatagaat aagaaatcat atttataaaa cctgatttaa ctgcttctgt gactaccata
    11941 tcagcttgat atcccattga gttcattaaa actgttctta tagtagttgc aagtaatact
    12001 cctacttgta ttgcaactgg atatagtgac atacttaagc caggtaagtc ttttcctgat
    12061 ttccaatgcc cataatcaca agaaccggca aagaaattaa gatttattat tgatactaca
    12121 tttgaaaaac ctccaccaat tgccaacaac atagttactc ctaaagtagt tttcccccaa
    12181 ggagtccatg caaccaataa gtggcatagt gcacatatag aaaaagaaat tattgaagct
    12241 agtttttgct ctctacagat ttttataaac actggtgcga aaaacatgaa tgctacactt
    12301 accaaaggca tgattgataa aaatactgct aatccacgtt catcttctat tacatattta
    12361 aaataatatg gagccaaagg tcctaatcct atagatctta tacatataag aaattctaaa
    12421 caaaatgcca tcataagtgg tacattagtg aataaagctt ttgccatttc acttgcttta
    12481 atctttggct tctcttcttt ttgaagaact tcttcttctg ctccagattt tattatctct
    12541 gttgaagaaa accaaaatac taaaaggcat cctgctgcaa agaataagta ggtacctaaa
    12601 taacccattc cttctccacc taatacatct gcaaataaaa ctaataataa cggatatgaa
    12661 aaacctacta agaatttacc aacttcttta aatatattcg tagtagttga gcctataact
    12721 cggtctctag gtgtcttagc aactacaggg taaatagcat aaattatagt gaaaactact
    12781 tgctgagcac aagatgctat taataataaa gctgtcatca aaggtaaaag taatgctgga
    12841 cttattttca ttaatggaac tatgaaaaat attccatatg ttaatgctaa cgctgctggt
    12901 gcatatctaa gccatggata atactttcct tttttgaatg gttgtttgtc aaatactgta
    12961 cctgttatag gagctacagc aatttttatg aaagattgta taatttgcat accacctgcc
    13021 ataattacac caagttttgc cacatcagtt aaaaaaatat ttgtaacagt tgatacaaag
    13081 taacctataa aattgtacgc taaatttata gaacttgaat atgtgtatat tgctcttttc
    13141 ttagggtctc tcatatcatc tctaaaattt gatataagat ttccttttgg tttgctttct
    13201 tgactactca catctacccc tcccttataa aatttactta ctttttatta ttcacgtata
    13261 ataatatata ttttgaatct taaatatctc attcttgatt caggagtata tttttatatg
    13321 tgtataattt taatttaatt atattctcaa aattcataat tgtcaaacac tttttaagtt
    13381 ttatttttta ctactaaaat taactaaaaa gctatttaga cttaatttag gtttgttttt
    13441 tattattttc ttgttttgaa atgtcattga aatgcgtatt ttaaattatt ttatcatatt
    13501 tctagtgatt ttaatttttt ataattttat tcaagaataa tttattcaag atatttctat
    13561 actacatcca gatattaaaa atatcttttt cttatttaaa ttccttttat ttttatacat
    13621 gtactattga tttaaaaata aaagcgtgtt acaattacaa aaaagaatct atgagataaa
    13681 tatcaatatg caaaaatttc agaattttca attttctatt tttacgtaat ttccatctaa
    13741 tatcataaat tattatagct atattaatct aaaaccaata aaacaaatat cgtactactt
    13801 ttatataaaa tatatttatg tacagtgcat attaaaaatt ccaatttcta attttatcat
    13861 gattatttta tataatagtt ttttaaagaa ttaataaaac atagatttag agaataaaat
    13921 attcttgact atattaattg tttaatataa tatagttgat atagtcaaac atattataat
    13981 tatagaaggg aaatttaaga tggattttaa aaatttacag tatgaaagtt tgtcatatat
    14041 tatttgtaac ttgcaaaaaa actttaaact ttattgtgaa aaatgtttaa aaccatataa
    14101 acttacgaat ggtctatatt tctatttaat ttatattaat aaaaatagga attgttcttt
    14161 gaatgatgtt tctacagaat ttgaagttga taaagctcat acaacaagaa ctatatctag
    14221 gttagaacaa gatggataca tagaaaaaat ccaaaaccct aatgatagta gagcttttca
    14281 gttgagagta acagataagg gtgaggaagt attaggggat ataaaaaaca tattttcaaa
    14341 gtgggataat cacataaaaa aagagttctc ggatacagaa tataaagaac tggtaaagaa
    14401 tttacatgtg gtaaaggata taaaaacagc tgtggaggaa gaataggatg tattgtaatt
    14461 tattaaaacc aattaaaatt ggaaatttgg aattaaaaaa tagagtatct tttgcaccta
    14521 caagcatggg tcttaaattg gaagaaaaaa ttaaaaagtt ttctgatatt gctaagtcag
    14581 gagtagctct gattactcta ggagatgtat ctattagacc tagttttcat aaggtagcta
    14641 taagcctatc ggatgaagat ggagtcatga aatacaagaa aattgtagat gaaatacaca
    14701 attctggtgc aaaggtatca gcacaactat tttgttcaga ttatgatgtt aatttaatta
    14761 aagatactat gaagatggga attacttcac atgatgagat aaaaaaaata atgaatgacg
    14821 gagtaaaaga ttacataaca aatatgccca aagaagaaat taaaaatatt ataaatttat
    14881 ttaaagtaac agcattgaat gcaaaaaaag caggttttga catgattcaa attcatggtg
    14941 ataggcttgt aggaagtttt tcatcaagta tatttaataa tagaaatgat gaatatggtg
    15001 gaacgtgtga taatagaagt agatttgcaa gcgaaataat tagtagtatc cgagatgaag
    15061 ttaaggacat tccaatagac tataaatttg caataagaca agaaaatcct cattatggaa
    15121 atgcaggagt tttattttcg gaagttgagt attttgttaa aaaatttgaa tccctaggtg
    15181 taaacagttt tcatgtaaca cttgcaaatc attcaaaatt agaagatact ataccaacca
    15241 ataatcatcc ttactttaaa gatgaaggct gttttttata tttagctgat gaggtaaaga
    15301 aacacacaaa tcttccagta tgtggggttg gaaaattgtc tagcccagat tttatagaaa
    15361 gtattatttc aaataataga gtagatatgg tttctatgtc aagacaatta ctagcagata
    15421 gtaattggtt acagaaagta aaagatggaa gagtagatga gattaaaaag tgctgttatt
    15481 gtaataagaa gtgtgcagat gctttacaaa cacgctctca gtttggatgt atactagatt
    15541 agaatatcct tataaaaata aataatttgt taaggttaat atatttttgg tgatttagat
    15601 aagtatttag ataaagagga ggagacatca tgaaaagttt agttttatat tcaagtttaa
    15661 ctggaaatac taaaaagatt gcttatacta tatatgatga aattcaagaa gaaaaagaca
    15721 ttaaagatgt aaatgaatta gttgattata gtattgacta tgaaaattat gatatagtat
    15781 tcttaggcta ttgggtggac aagggaattt gtgataaaaa ttcaaaacaa gtgcttgaaa
    15841 atattcacaa taaaaagata gcactatttg gaacaatggg agcaagtaaa aaaggaagtt
    15901 atggagctag tataatagaa aagatagaat caattatccc aaaagataat gaaatattag
    15961 gttcattcat atgtcaaggt aagatagcag aaggattgaa agcaaaatat aaagagatgt
    16021 taaaactttc tcctgacaac gaacatattc gccaacaatt aaataaccat gaggaatctc
    16081 aaagtcatcc agatgaacaa gaaatatatg aagccagtat gtttgcaaaa aatatgatga
    16141 ttaaagctag tattgtgtag tagttaaaca ataagttcat ataagatata gaataaataa
    16201 tttagtaaaa aagtatagca aataatttgc tatgcttttt ttatgaaatc aaaaaaataa
    16261 taaaaatact tgactgtaaa ccttgtttat acattatttt atagaaaata agatagtttt
//
LOCUS       DAMJYC010000089        15979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJYC010000089 DAMJYC010000000
VERSION     DAMJYC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.091"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC472_003037"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC472_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2728346.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC472_003038"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC472_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2728347.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC472_003039"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC472_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2728348.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC472_003040"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC472_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2728349.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC472_003041"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC472_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2728350.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC472_003042"
     CDS             2662..3168
                     /locus_tag="PC472_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2728351.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC472_003043"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC472_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2728352.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="PC472_003044"
     CDS             3983..4771
                     /locus_tag="PC472_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2728353.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5242..8958
                     /gene="rpoB"
                     /locus_tag="PC472_003045"
     CDS             5242..8958
                     /gene="rpoB"
                     /locus_tag="PC472_003045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2728354.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9000..12485
                     /gene="rpoC"
                     /locus_tag="PC472_003046"
     CDS             9000..12485
                     /gene="rpoC"
                     /locus_tag="PC472_003046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2728355.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12774..13196
                     /gene="rpsL"
                     /locus_tag="PC472_003047"
     CDS             12774..13196
                     /gene="rpsL"
                     /locus_tag="PC472_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2728356.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13320..13790
                     /gene="rpsG"
                     /locus_tag="PC472_003048"
     CDS             13320..13790
                     /gene="rpsG"
                     /locus_tag="PC472_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2728357.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13839..15905
                     /gene="fusA"
                     /locus_tag="PC472_003049"
     CDS             13839..15905
                     /gene="fusA"
                     /locus_tag="PC472_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2728358.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6255 a         1989 c         3251 g         4484 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa acaatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgagag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgttttata aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgag gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggagat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaacatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt atagtagaaa tgatattttt ataaaggcca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt tgtatatttt
     4921 aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat atggaattat
     4981 aattaaatag gggggatggt acttgtataa aattttttat agaaaattag acctataaat
     5041 gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg cttaaaataa
     5101 tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata tattgaaaaa
     5161 tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt ttatttttaa
     5221 ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag aactagaatg
     5281 agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat tcaagtagat
     5341 tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat ttcaccaata
     5401 gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga tgataaacca
     5461 aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc tctaaaggtt
     5521 aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt gtttatgggt
     5581 gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga aagagttata
     5641 gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga taaaacaggt
     5701 aagagattga tttcatctac tgtaattcct aatagaggag catggttaga atatgaaaca
     5761 gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc agttacagtt
     5821 ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt aggagaagat
     5881 gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga aggtcttgta
     5941 gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc atcatcttta
     6001 ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg tagatataaa
     6061 ttcaataaga aacttgcttt atgttataga ataatgaata aaatttcagc agaagacata
     6121 ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag ctatgatttg
     6181 gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga tgatgataaa
     6241 aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga ttttgacata
     6301 gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat tttagatgga
     6361 tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga acttatacca
     6421 aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa tatattctat
     6481 aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag atctgtaggt
     6541 gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt tataaaagaa
     6601 agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa cataagacca
     6661 gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt catggatcaa
     6721 acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg gcctggaggt
     6781 ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca ctatggtaga
     6841 atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc tctaggaact
     6901 tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt tgataaagaa
     6961 acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga tttattcgta
     7021 agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca tagagttgtt
     7081 tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga ttacatggat
     7141 atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt agaaaatgat
     7201 gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc tctagttaga
     7261 agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga ctctggagca
     7321 gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga aataattata
     7381 aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg ttcaaactca
     7441 ggtacttgta taaatcaaac acctataata aataagggtg accagataat aaaaggtgat
     7501 gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag aaactgtctt
     7561 atagcattca tgacttggga aggttataac tacgaggatg ctatattaat aaatgaaaga
     7621 ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg tgaagctaga
     7681 gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg agatagtgca
     7741 attaagaact tagatgacag aggtataata agaataggtg cagaagtaga ctcaggtgat
     7801 atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga ggaaagatta
     7861 cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact taaagtgcct
     7921 catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa tggagatgat
     7981 ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag aaaaataaaa
     8041 gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag agtattacct
     8101 gaagaagata tgccattcat ggaaaatgga acaccactag acataatact taacccacaa
     8161 ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg acttgctgct
     8221 aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga gtatgacata
     8281 atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt atatgatggt
     8341 agaacaggtg agtcttttga caacagaata actgttggat atatgtacta cttgaaacta
     8401 catcacttag tagatgaaaa actacatgca agaagtactg gaccatactc attagttact
     8461 caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga gatggaggtt
     8521 tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac tgttaagtct
     8581 gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga aaatatacct
     8641 gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag tttatgctta
     8701 gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc tgtggatgaa
     8761 gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga agttgaagaa
     8821 agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga tgaggatata
     8881 gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga ttttgatagt
     8941 gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga gagaactcct
     9001 tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca gaaaaaataa
     9061 gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt actttaaaac
     9121 cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac tgggagtgcc
     9181 actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga tgtggagtag
     9241 aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta gctgctccta
     9301 tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta cttgatatgt
     9361 caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt gatccaggag
     9421 agactggatt aaacgaaaaa caattgctta cagaaaaaga atacagaact gctcttgaaa
     9481 agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca ttactacaaa
     9541 atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat agtacagggc
     9601 aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa aagtctggaa
     9661 ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat ttaagaccaa
     9721 tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta tatagaagag
     9781 ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca gatataattg
     9841 taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat aatggtagaa
     9901 gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca gatatgttaa
     9961 aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac tactcaggac
    10021 gttctgttat agttgttgga ccagaactta aattttatca atgtggactt ccaaagaaga
    10081 tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa ggttatgcac
    10141 ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt tgggatgttt
    10201 tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact ctacatagat
    10261 taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa ctacatcctc
    10321 ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt catgtacctt
    10381 tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat aatatacttg
    10441 ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta ggttgctatt
    10501 atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt aaagacttta
    10561 atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta gtgaaactta
    10621 aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt agatttatat
    10681 ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat ccatttgcac
    10741 ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat aaatgcttta
    10801 gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct ctaggattta
    10861 aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc ccagaagaaa
    10921 agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa gcatacagaa
    10981 gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg acagaaacaa
    11041 ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat atatatataa
    11101 tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca ggtatgcgtg
    11161 gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct aatttccgtg
    11221 aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa ggtcttgccg
    11281 atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt gatgtaagtc
    11341 aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag atttatgcta
    11401 taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga agatatacta
    11461 tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat tcaatgatac
    11521 aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg ataagaacag
    11581 ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga aacttggcaa
    11641 caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa tctatcggtg
    11701 agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca ggagctgata
    11761 taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca aaaggattag
    11821 ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag agaaaagaag
    11881 taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat ggttctagat
    11941 taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa ggatttataa
    12001 atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat atagttaaag
    12061 aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat atagaagtta
    12121 tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca gatttattgc
    12181 caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct atagataaag
    12241 gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca tctcttgcaa
    12301 ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta acagaggcag
    12361 caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata ttaggtaagt
    12421 taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa aaaattgaag
    12481 attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt taaaatagta
    12541 aagtgtgttg tttaaaagta cactaagggc ttgaacaagt tcttagtgac ttttagatgg
    12601 caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca tatttatgtg
    12661 ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa tattcatatt
    12721 gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag atgatgccaa
    12781 caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct actgctccag
    12841 cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt gccccacaaa
    12901 aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac tcagctttaa
    12961 gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat ataccaggag
    13021 aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga gtaaaagacc
    13081 ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt gttgataaga
    13141 gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa aagtaaaaac
    13201 aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg catcacggca
    13261 ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag ggaagcgaaa
    13321 tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg tatgggagta
    13381 aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga aagtctcaaa
    13441 gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa gctttagaag
    13501 tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca agaagagttg
    13561 gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa acattaggtt
    13621 taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt gaaaagctag
    13681 ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa aaagaagata
    13741 ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag gatatataga
    13801 tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag tttcctttgg
    13861 aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact actactacag
    13921 aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat gaaggagctt
    13981 ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact tctgccgcta
    14041 ctactgcttc atggaaagac catagaataa acataataga tactcctgga cacgtggact
    14101 tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct gtattctgtg
    14161 caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa acttatggag
    14221 tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc tacaatgttg
    14281 taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta ccaataggta
    14341 aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt atatacaaag
    14401 acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa gaattagctg
    14461 ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa ttaatgatga
    14521 aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata agaaaagcta
    14581 ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac aaaggtgttc
    14641 agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata ccagctataa
    14701 aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa gagccattct
    14761 ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg ttcttcagag
    14821 tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag aataagagag
    14881 aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata actaaagtgt
    14941 atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga gacacattat
    15001 gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca gttatatctg
    15061 ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct cttcaaaaat
    15121 tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga caaactataa
    15181 tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta agagaattta
    15241 aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata actcaaccag
    15301 ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac ggtcacgtta
    15361 agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac aaaactgttg
    15421 gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa ggagctatgc
    15481 aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta tatgatggtt
    15541 cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca atggcattca
    15601 aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa gtagaagttg
    15661 ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga agaggattaa
    15721 tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt ccactttcag
    15781 aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca acttacacaa
    15841 tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata gctgaaggaa
    15901 aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga tgagagatat
    15961 ctctcatcta gcacaagat
//
LOCUS       DAMJYC010000090        15801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJYC010000090 DAMJYC010000000
VERSION     DAMJYC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.092"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            102..1292
                     /locus_tag="PC472_003050"
     CDS             102..1292
                     /locus_tag="PC472_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2728359.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1462..2424
                     /locus_tag="PC472_003051"
     CDS             1462..2424
                     /locus_tag="PC472_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2728360.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2543..3205)
                     /gene="zmp1"
                     /locus_tag="PC472_003052"
     CDS             complement(2543..3205)
                     /gene="zmp1"
                     /locus_tag="PC472_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2728361.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3305..3390)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3575..6493)
                     /locus_tag="PC472_003053"
     CDS             complement(3575..6493)
                     /locus_tag="PC472_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2728362.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGILKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYEVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENIRQENPPVNPPAPPNTDEPMVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNRVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6611..6696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6823..8001)
                     /locus_tag="PC472_003054"
     CDS             complement(6823..8001)
                     /locus_tag="PC472_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2728363.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8376..8561
                     /locus_tag="PC472_003055"
     CDS             8376..8561
                     /locus_tag="PC472_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2728364.1"
                     /translation="MFFISDIYIKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8626..11400)
                     /locus_tag="PC472_003056"
     CDS             complement(8626..11400)
                     /locus_tag="PC472_003056"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2728365.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11572..12828)
                     /locus_tag="PC472_003057"
     CDS             complement(11572..12828)
                     /locus_tag="PC472_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2728366.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGIRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12900..14402)
                     /locus_tag="PC472_003058"
     CDS             complement(12900..14402)
                     /locus_tag="PC472_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2728367.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14493..15683)
                     /locus_tag="PC472_003059"
     CDS             complement(14493..15683)
                     /locus_tag="PC472_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2728368.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4957 a         2738 c         1923 g         6183 t
ORIGIN      
        1 tatattttca aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata
       61 tatacataag caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc
      121 aaactttagt tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt
      181 ctgatattta tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac
      241 ctccagagac tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag
      301 ttcatggata tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacaca
      361 taaataaaaa agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg
      421 ctggtggtct aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt
      481 ttgcaccgta ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag
      541 aagttcctac aaatatagag acttttgagc cagatttaga tgctctaaaa aatgctatta
      601 ctccaaaaac aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt
      661 ctgaagaatt attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata
      721 caagcatata cctaatttct gatgagccat atagagaaat aatttatgat ggtgccaaag
      781 ttccttgtgt actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat
      841 tatctctacc tggtgaacgt ataggatata ttgttgctaa tgggcaaatg gatgattttg
      901 atgatgttat gtcttcatta aatgttgcaa atagaatact tggatttgta aatgctcctt
      961 ctctttttca aagggttatt gcaagaagtt tagatgcaga agttgatgta aatatttata
     1021 aaaagaattt tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac
     1081 ctaatggtac attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca
     1141 atgatgcaaa acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc
     1201 attttagagt atcatattgt gtatcttatg ataaagttaa aagttcttta ccagcatttg
     1261 aaaaattagc caaattatat aatttaaaat aactaaatca aagtaattca aataattaac
     1321 taccttagta tagaatactt acattctata ttaaggtagt ttataatttt atgtactata
     1381 ttttatacat accatattat tccaatatgc ttgaatattc ttatctatag tactaatata
     1441 aattacaagg aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta
     1501 ttttttagtt caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa
     1561 ataactttat caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga
     1621 ttaagagctc ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt
     1681 tttatgtgtg tagtagtaag tatgtcattg tatcaactgg ctgttctaaa cacaaaggca
     1741 tctgtagttg ctgttttgtt cagttgtaat ccagtctttg taatgctttt agcttactta
     1801 attttgaagg aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga
     1861 attatagtta taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca
     1921 ttatcttctg ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa
     1981 tttggtggaa tagttgtaac ctgctttagc tttatatttg gtagtttgga aatgttgata
     2041 ttggttttac tcactaaaat taattttatc gcagatatat taaataaaaa taagcttcat
     2101 gtatttgcaa atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt
     2161 tatgtattcg tatttgttac aggtgttggt tatgcgcttt actttatggc aatggaggct
     2221 acatctacta gtcttacatc tttagtattc ttctttaaac cagtaatatc tccaatacta
     2281 gcattattaa tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata
     2341 gtaattggtt caataatttc tattatacct acgataatta ctcaaatgca taataaacac
     2401 gttgaagcct tgtataagga gtagtaattg ataatctatc atagttatac ataagacaaa
     2461 atttattaga tttaaacgca atataaaaga gatgctatta taatagcatc tcttttatat
     2521 ttattttaaa tttattaaaa ctctatttag ctaaattttg caaaaagcta taagtttgtg
     2581 gacaagctga ttttaactta ctatttgtat cttgatttaa atagtaatat gcaaatgatt
     2641 ctgcaaaaaa ttcttctgga tatacaccta agtagtttac attacctaaa gaacgacctt
     2701 ctttagcaaa tatttgtttg aattgtgctg atttagatat atcatttaat actatatggt
     2761 ctattgcatg tgctgtttca tgtaattcta aatttattgc atcatgccct ttacctttgt
     2821 tactaaatcc tattctaaga gctactgtac ttccacctaa acctggaaca tcatcccaag
     2881 tttttccagt accttcccat cccttaggaa ctacaccttt taaataagca tattcttttt
     2941 catctgttaa ctttccactt aataatttta tttttaagtt attttgtttt aatgcattta
     3001 aatactttcc atctatatta gctaatctat ttaccatagc attagcttcg tttttatcgt
     3061 aatttccagt tggaaaaact actatttgac taagtgtgtc tttattttgt tgtatagtag
     3121 tactatctgc atgtgcactt actggaactg aacttattaa gaatattgat attatagcta
     3181 ttaataattt tttacttggt ctcatattat tccctccttt gagttaggcc gggagtaata
     3241 aatttcaaaa taaaaaagat acccaagagt atctttgtat atacaaaaag aaatttttac
     3301 tcttttcggc aactggctgg catggtttac taaactttag cccctatagc tttgcgtgac
     3361 taggtttccc tagttttgcc tcatttaatt ttatatctat attatagctt atatcttttt
     3421 aattgtctat atccctttgg aacttttttc taaaaagtta ttattttttt acataaatct
     3481 tcaaaaaaaa atatcttcta acaatatttt cattattaaa tattgtttta gaagatattt
     3541 tttaatataa cttatatttt tataatgtta gggtctaatt tgtattttta tttcttctta
     3601 atacgataag tcctacaata gctcctataa ccaaaagtat ttctccaata attgtagtac
     3661 tatcccctgt ttttggtggt actggtggat ttaccaaagt atcatcttta actctattat
     3721 ttttttcttc tgacactgat ggctttacta cttctggtat tggaataatc tgtttaacag
     3781 gagtttctat catctcttct gatggtggta ctggcggatt caatacctct ggtattggtg
     3841 gatttattat atcttctgat ggtggtactg gtggatttat aactatagta tcctctgtat
     3901 cagatgaact tgatggtttc cttggtttat ctgtgcttgg tggtactggt ggatttacca
     3961 ttggttcatc tgtatttggt ggtgctggtg gatttactgg tggattttct tgtcttatgt
     4021 tttctatatt cccaatatct actatcttac caatatcttc attaccaatt tcaaccttaa
     4081 ctttatcctt cagtttttca tatccacgtg gagccattat ctccactaaa taatatgttc
     4141 ctgggttttt tacactagat tgaattatac catcttcatc actaactaaa tccttaacta
     4201 ctatatttcc aaatgcatct tctaaattga acttagcacc tgaaagtaca tttttatcta
     4261 aagcatccac ttttttagct tcaaaagcat aaccttgctt agtagtctct actcctggag
     4321 gacttgaacc attaatctga gccacatttc gtatagtctc cccaactttt acatttgtga
     4381 agtttgcatc aaatgtaacc caactttgca caggttcccc ttcttttatc tgtttaaatt
     4441 cgtatttatt tactaatctt attgtatttt ttccatctcc agcttctttt ttaagttcaa
     4501 gattatcaga atattttata ttagatattt ctagaactga gtttacttca tctaaaagat
     4561 ttacttgtcc tgcatcataa actccataag ttgacatatt gattttgtat ttaacaatct
     4621 ggttatcact actgtttaaa acttttttat caacttcttt ttttgcgctt attaatccag
     4681 aatttttctt aaagtttatc tcatcatttt tatcaatttc actattattt gcatcaagaa
     4741 cagctctatt tatatagcta tcttcaatac taattacctt taatgtataa gctataaaaa
     4801 actttgattc cacttcttct ttactgagat taattgtaaa tccagttata actttatcta
     4861 cttgcccact ttcatttctt ttttcttcta cttcatatga aatattattg gcaatttttt
     4921 ctttactcca aggagtccag ctcccattca ttggaatttc tacaactcta aaatcatgcc
     4981 cagcttctcc tgaagtagtt aatctcatgc cttctggtat attatctgtt ataacaacat
     5041 tacttattgg tgatttatca tcatttattg aaatctgata gtttattact tgaccaactt
     5101 gagtaatatt tgttgctcct gaatgtcctt tagtaagaag ataatcatct cctctatcaa
     5161 atatacttac agtagcatca tcttcttgta tcttcttacc atcagattca atcctagcta
     5221 cattcttata tttattacca tgttttgcat ttttgtcact gattctagcc ttatacttaa
     5281 cagtatacga tttatctata tttccaaaat taactgaaat agaattttta ctctgttcaa
     5341 tattaaaatc actaacttct tttccatcac ttgttactac acttacagaa ccatttatca
     5401 atgtcaatcc ttcaggaagc tcatcataca aaataatatc ttttaactct tcctgctttt
     5461 catttacttg tatactgtaa tttatatcct tatttttaac tggcataaat acagtagttg
     5521 atttttttcc ttcaacaagc tttttaagta ttcctttagc atctggaaca tccgttttat
     5581 ctccaggttt tatttctaca tctatatctc cacttccact ttctattttt ccagtagttt
     5641 caatatgtat ttttgcatta gtatcttcat caaccttttt aacatatgct tccaaaccta
     5701 ttttaccttg cattacactt tggccattag gtaattctct aaatgtaagt gtatactctc
     5761 cattactttc tgttttatca aagcattcac ttgtataatc taaattaact ttattgaata
     5821 catctggcac cttaaaagtt attgaatctc ctgcttgtat tccttttgat gttggagtat
     5881 aagttaaatt aaatgatatt tttttcattg attccgtttc tattgtattg ttattaattt
     5941 ttacaacaaa tccatagtct tcattttctt cattaagttc ttcttctaaa actttcaata
     6001 aattttcatc ttctaataga ttatcttcct ctggagtttt ttctgtattt ttattgtcta
     6061 gtggtttttc atcttctaaa gcttgttctg actttttatt atctgttgac ttatcttctt
     6121 cttgtatctg ctctggcttt ttattatcta ttggcttttc tccttctgga gtttgctctg
     6181 gctttttatt atctgttgac ttatcttctt ctggggtctg ttctggtttt ttattatctg
     6241 ttggtttttc ttcctctggg gtctgttctg gttttttatt atctgttgcc ttatctttct
     6301 ctggagtctg ttctggcttt ttattgtcgc tttccttctc ctctccagtt gatgaattag
     6361 ttgaaccact ttgaatctga ctattctctc ctaattctga agcataagat acagttggat
     6421 atattgtaga caccaccata cttaaaatca taattaaact aattaataat gcctttctat
     6481 ttcctttctt catgtaaatc tcaccccttt aggttaccta taatttataa tctaatcttc
     6541 cattaatatc atatataaag taatttttac taaaaaatta tacaaaaaaa gctatgaaaa
     6601 taaataaatt tatattcata gccggttgca ccactgactc agtatatcca taaggtgccc
     6661 aataatagaa tatacttcaa catctctata atatttcata aaaacaatta aaattgcttt
     6721 atttatatta aaaatacaat tatatacttc tattatatta tgcctctata ctattgtcta
     6781 tcctttttac attttttacc ttatgatgag attaattaat aattaaataa aatccttatc
     6841 tacaacaatt acacaattat attcaggatt tatctcctta actaattttg ttatatcttc
     6901 ttttaagtca tcttctgaca tgtttttgga aaattcggaa ggattaacta ctatatcaaa
     6961 tattaaattc ttcttgcttc cttttccaac tattctaaaa tcatgcatag attttacaag
     7021 actatttttc ttaagtattt cttcaagttc ttttttggct gacataactt ctttatcttc
     7081 aagacatatt gggtccatgt gaattactag atatatatct aatttttcag atatttctct
     7141 ctctgcagta tctataattt catgtatttc cattatatct atattggatg gaatttctgc
     7201 atgtatagat gcaatacatc taccaactcc atagttatga ataattaaat catgtgtacc
     7261 tattatatat ttataagata acaatagctc tataatagaa tttaccattt cttcatccgg
     7321 tgcttctcct aaaagaggat ttatagtatc ttttacaaga gagaaaccag cataaagtat
     7381 cactaatgat actacgatac caacatatcc atctatggga aaattagtaa atctagctac
     7441 aataaatgat ataactacac aacttgaagt aaatacatct cctaaagcat caagtgaaac
     7501 tgcttttaag gctgaagaat caattttatt tcccataaat ttattaaaac gactcaacca
     7561 gattttaatc attatagatg caattaaaag tataagtggt attacttcaa attttacagg
     7621 aattggattt actatccttt ctatagatga ctttacaaat tgaagtccaa ccaacatcac
     7681 cataaatgct actattaatg ctgataaata ctctattctt ccatgaccaa aagggtgttc
     7741 tttatcagct ggcttacttg ctaatttaaa acctatcata gttattgctg aagatgccat
     7801 atcagaaaga ttattaaatg catcagccat tatagctata ctagatgtga gcattcctat
     7861 gaataccttt atcacaaata aaagtaaatt ggataatata cctactacac cagctacata
     7921 accatactta tttctaacat ctgtattttg aacgttttcg ctatctctga taaatgtttt
     7981 gactaggatt ttggaaaaca tgaaacttac ctccctgccc tttattttat taacaaattt
     8041 tataatctat atctttattt atgataacat aatttatata taaaattctt aaaaatcact
     8101 ctactccagt aaaatatcat tctatatatc tatttagata cgaattttat atttcgtaaa
     8161 gtttttatgg cttagtgtct gtttaataga ttctatataa gtttttacaa agattattta
     8221 ttatgataat atcatttttc ccaagtatta cttagaatat gatttttttg ttttgtctgt
     8281 acatatttta aatttatact atatttatct atattttata accttatgat acactataac
     8341 tataagattt taaatggaaa ggaatgaaat aaatcatgtt ttttatcagt gatatttaca
     8401 taaaatcacc tattaaattt gatactcctt tgcaaaaaga ggcatacaaa atattacaaa
     8461 aattagatat tgactttgaa tgtgttgaca cagatgaagc aattacaatg gaagattgtg
     8521 ttcagattaa taaaaaatta aatatgaaaa tggtaatata aaacaacatt aaacaaacaa
     8581 aaaccgcaaa gttacaatat atagtacttt acggtttcat aagaattaat ttttactatt
     8641 aaagttcata gttctactat ttttcattgt tctaagaatt agctttacaa attcatttga
     8701 taataggata gtagatgcaa atagtattat ctttatccac atatcaaagt ccaaagaaac
     8761 agaattaaag aaactctgga acacttgtgt aaatattatt tgaattatcc ctgtaactac
     8821 tattatttgc aatgccagtt tatttttaaa gaaatttggt attgtactat ttaaaccaaa
     8881 ctctctacaa tttaatgcat taaaaagggc actgaatgca aataaagaaa atataacagt
     8941 accttgttct tctgatgtag ctcccaatat attaaaagct gattgagtaa atacaattgt
     9001 aattataaga atagcattta taataatatt tacaaacata gacctagcta ttattccaga
     9061 atgtctattt attggttttc ttttcaatac ataatctcta acaggttcta atcctaatgc
     9121 tagggctgga ggaccatcca ttattatatt tacccataat aattgtattg tagtaaatgg
     9181 catatcctta ccagtaagtt gagatattat agcaattata aatgctacta tatttacagt
     9241 aagctgaaac tgtataaatc tttgaaagtt ctcatatata cctcttcccc attttattcc
     9301 ctctactata gtactaaagc tatcatcagt caatattata tctgctgcat ttttagatac
     9361 ttctgttcct gaaataccca ttgcaattcc aacatctgct tgactaagtg ctggggcatc
     9421 atttatacca tcacctgtta cagctacaac ttcgccactt ttttgaagtg ctgaaactat
     9481 tctcattttt gtatctggtt tacttcttgc aacaatagat attcctttga tttcttctct
     9541 caattcttta tctgtcaata catctatata tgttgcttca actgcctttt tcccatcatt
     9601 taataatcct aattcatttc caatagctgt tgctgtattt atattatctc ctgtaagcat
     9661 ttttacatct actcctgcat taaatgcttt atcaatggaa tctttgaccc cttctctcaa
     9721 tgggtccact attcctacaa atccactaaa aactaaatta tcttccttca tataacttct
     9781 tgaatttcct attaaattca attcaccttc tgaagtcatt gcaacttctg ttttagaatt
     9841 actcattttt ttataagcaa atcctaatgt tctcatagat ttaatttgta acttcttaat
     9901 ctcatctaaa atactttttt ctacttttgg agttattgga acaatatttt ttccttgttg
     9961 tacataagaa cattttttta ataatacttc tggagcacct tttgaaagta atatatctga
    10021 atcctcttga cgaattaatg ttgacatctt cttttcttct gaactaaatg gtatttgtga
    10081 tatcaaatac gtttgttttc tcatttcatt atagttctta tcattatgat ataaaagtaa
    10141 tgcacactct gttgcgcttc ctatatattt aaatgaatta tcttcttttt ctatatctgc
    10201 agttgagttt actatacagt tttgttcaaa atagctatta ctttgatact ctcctccact
    10261 tatatattta ccatcaacat atgctacctc taccatcatt ttgttttgtg ttaaagtccc
    10321 tgttttgtcg gaacatatta cagaaacaga acctatagtt tcacaggctt ctttctttgt
    10381 tactaatgca tttatctttg ccatcttttg cattgttata gctaaggtaa tatttatcat
    10441 agttggcaaa ccttctggaa cagttgcaac tattaatgca atacaaacca tatatgcatt
    10501 tttagcaggt tctaatgatt gtaagaaaga taatatactg cttgtatcta aaattaacat
    10561 accttttctc tgcatctgta taatcataaa catacacaat aaagtagcta ttatgcctga
    10621 tattcctgct attttagcac ccaacttccc taatttaatt tgaagtggag tttgctggtc
    10681 ttcatttgat aaattctgtg ctatcttacc catctctgtc ttatcaccag tatgagtgac
    10741 aaccattatg cccctaccat atgctacaag agtccctcca aataccatat tgacttgctt
    10801 tgcaggtatt gcatcctgtt cgattatttc tgttttagaa taaactactt ccatatctaa
    10861 tacagcatca gcattcttag ctacatcatc agattctcct gttagcatat cttctctaag
    10921 tttcaaattt atagactgta taagtcttcc atctgctggt atcatatccc cagtttctat
    10981 atatacaata tctcctggta ctaaatcatt ttttgatatt ttaattatct tcccatttct
    11041 aagcaccttt acttctatat tttctgttaa ttttgacagg gcatgagctg cttttttaga
    11101 ttttccttca gttattatgc ctatagaaat tccaataaaa atagccccta aaatacctaa
    11161 tgcgtcatgt aattctccca caaaagaact tattactgct gctcctatga gaattaatat
    11221 cataggttct gtcaaacttt ctcccaactc gtcccaaaat gttctacctt cttttatagt
    11281 aaattcattt aatccatatc taagcttcct ttcttctacc tcattatcat caagtccaat
    11341 ttctggattt gtttttaagt attttaatac ttctttggta ggtttattat aatacctcat
    11401 aactttatcc cccttgttaa ttatctataa ggaattatat gcttatgata gcttgtatat
    11461 tacttttcaa aaaaaataag gtgttgatta atcaacacct tatagtggtt atatattatt
    11521 aaattttatg ttacaggctt tataaattgt gagctataca aatcaaaata tttaatcttc
    11581 aaaataccct tctggatata atggaaactt agatataagt tcttgtgcct ctgctttaca
    11641 ttcatctaat acttctttat tatcaatatt ttctgctacc ttattcatta tccctgctat
    11701 ctgtataact tctttttctt tcagacctct ttgagatatt gatgtaagac ctatacgaat
    11761 accactagtt acaaatggtg attgtgggtc gtcaggtata acatttttat taactgttat
    11821 tcctacatat tcaagagctt tttcaaattg ttttcctgta agattcttac ttcttaagtc
    11881 aaccattact atatgattat cagtacctcc acttacaata ctaaaaccat gtgaaatcag
    11941 ttcagaagct agagtctgag catttactac aacttgctcc attatctttt taaatttatc
    12001 tgtacttaaa tataatagtg aaaaggcttt tgccgcaact gtattaagat gtatagaccc
    12061 taatgttcct ggaaaaacac ctttatcgag tttctttgca tgttctgctt tacaaagtac
    12121 aataccactt cttggtccac agaaagtctt tgtcgtagat gaagaaacaa aatctgcata
    12181 aggtacagga ctaggtatta ctttcgctgc gactaatcct gcaacatgtg ccatatctac
    12241 cataaagtaa gctcctactt cttttgcaac ctttgatatt ctttcataat ctattaatct
    12301 tggatatgaa cttgcccctg caattataag ttttggtcta cattcttttg cctttgcttc
    12361 aagagcatca taatctatac gtccagtatt tgggtctacc ccataaaatt catatttata
    12421 aatgtcatgt aaaaaattaa ctgcgctacc atgtgtcaag tgtccacctt ggtcaagacg
    12481 catacttaaa acagtgtcat ttggttttaa tattgatgaa tatacacaat agttagctgt
    12541 agaacctgaa tacggttgta cgtttacatg ttctgctcca aatacttctt ttgcacgttt
    12601 tattgctagg gtttcaagtt tatctgcttc ttcagaacct gcttgaaaac gagcccctgg
    12661 atagccttct tctgttttat ttgtaaatac acatccactt aattcaagta cttctgttgg
    12721 tgctgtactt tcagaagcta tcatctcaat attgtgttct tgtctaacca actcatcagc
    12781 tacaatcttg tataactctg ggtctgaaat ttttgttgtt tttaacataa taatcctcct
    12841 cttaattaaa gaatatattt tattagttta atcttcattg ataaatttat tataacggtc
    12901 tataaaacgt aagatattcc tccaccaggt cctaatggta taccaatacc catgaatagt
    12961 aacaatatta ttatttctat taatgtaaat accactgagt atggaatcat attcgatatg
    13021 acagtaccta tacctatatc tttatcatat cttctggcaa aggccaatat aactggaaaa
    13081 tacggaaaaa gtggacttat tggatttgtt gaagcatctc caatacgata agctatttga
    13141 gttaatgctg gattaaagtc tagtagcata aacatgggaa caaatatagg tgctaaaatc
    13201 gcccattttg cagaagcact tcctacaaat aaattgataa atccagatag caatataaat
    13261 cctattatca atccatagct aggtattccc gactctgcta gtaattttgc tcctgttatt
    13321 gataaaattg ttcctaagtt actaagatta aataaattaa taaactggga tgctataaat
    13381 gctaggacta tatatccacc catatcactc atactactac ccatcataga tactaaatct
    13441 ttgtcacttt ttatcttctt acttactttt ccatacacta atcctggagt taaaaatata
    13501 atagtgatta taggaactat acctttcatt aaaggtgcat tactagatag tatagaacca
    13561 gtctctggat ctttcataaa tgcattgtca cctatacata aggcaactat cactactact
    13621 acaactaaca atgataataa agcgtatttt atacccttat tttcaagttc actcaattcc
    13681 tgtatatcct tatcatttgt tgaatcatta tattttgtaa aacgaggagc tataaatttt
    13741 tcagttacaa atgctcctgc aaaagttaaa actatcgctg atataaaagc aaaataaaga
    13801 ttcattgctg gattgccttg gtaagatggg tccatcattt gagctgctgg tattgtaaat
    13861 ccagctaaca atacatctgt agtactaagc ataacatttg ctgcaaatcc ccctgcaaca
    13921 cctgcaaaag ctgcaaacat acctacaaga ggatgtcttc caattcctaa aaacacaagt
    13981 gctgcaagtg gtggtaatat tataaatcct acatctgctg ctgtatgtga taacatacct
    14041 gcaaatacga ttgctacagt tacaagtttt tgtggaactt tacttatacc ctttttcatt
    14101 aacacttcca taaatccaga tttctctgca acacctgctc caagcattgt aaccagtact
    14161 aatcctaaag gagcaaatga ctgaaagttt gtaactatgc tacctaaata tgattgaatc
    14221 tgctctttct ctagtagatt tactaccttt acaacttctt ttgtagaagg atgtatttct
    14281 tctactccta aattagatat aactgctgac aatattacaa caattataca tagtaataaa
    14341 aatatggtta ctgggtctgg aagtttattt cctgctctct ctacaacatt tagtgccctc
    14401 atcaataatc cactatcttt gtttttttcc tgtgaattaa ttttcctcaa actaataccc
    14461 cctcataaac tttatctcca gaatgctcta tcttaaattt tattttttaa tttatccata
    14521 taatttataa cagactgaca tagtacattt gtacctacta atatagaatt tttatcagct
    14581 ttaaatttag gattatgcca ctgatatata caatcttgtt ctttgttgcc tacccctaac
    14641 cagtaaaaaa atcctggggc tttttccatg aatatagaaa aatcttctcc tcctgtagaa
    14701 ggtattggat caactatagc atcttctgaa tacaaatcac acacagactt acaagctatt
    14761 gcgtacatct ctttactatt tataacagct ggtaaatcaa ataaataatc tagctttgca
    14821 gttgcttgat atccacgagc tatctcacaa actatatttt ctaaattttc tgatattttc
    14881 tttcgtactt cattactaaa gcttctacat gtgcctgtca tatctacctt ttctgaaatt
    14941 acattatttg cagtccctcc atttatagta cctattgata taacagctga ttctaatgga
    15001 cttatgtttc tgcttactat ggtttgaata cccattataa ttgcacttga tgctactata
    15061 gggtctattg ttctgtttgg tatacctccg tgacctccaa atccatttac ttctatctta
    15121 atagtgtcta ctgctgccat taacccacct aacttaacac ctatttttcc acaaggtata
    15181 tctggatgat tatgaagtcc aaatatagca tcaacttttg gattttccat tactcctcgt
    15241 tccactagca tcttcgcacc tttattgact tcttcggctg gttgaaatat aaactttaca
    15301 tttccatgaa gactatctct aatatgagac aatacatatg cacatcctac aaggcaagac
    15361 gtgtgtatat catgcccaca tgcatgcatt ataccatcat atctggattt atacttaata
    15421 tcaacctctt catatattgg caatgcatca atatctcctc ttagtgctac tgtgggacca
    15481 tcatacttcc cttttaataa tccaacaact cctgtttcca ttcctaaatc taatatttct
    15541 atgtctaaac ttactaataa atcttttata gcttttgtag tcctaaattc tttaaaactt
    15601 aattcggggt gcatatgaat atctgtaaaa aaatctaaaa tatcttcttt atacttttct
    15661 gagattaaaa taagttcatg catactaaca cctctccaaa attttatttt tttatttttt
    15721 ataatttaac tacaaattat ctaagttaat aatctttata aagatatttt ttataaattt
    15781 aattttagtt tatatttata t
//
LOCUS       DAMJYC010000091        15670 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJYC010000091 DAMJYC010000000
VERSION     DAMJYC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.093"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(106..966)
                     /locus_tag="PC472_003060"
     CDS             complement(106..966)
                     /locus_tag="PC472_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2728369.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1283..2611)
                     /locus_tag="PC472_003061"
     CDS             complement(1283..2611)
                     /locus_tag="PC472_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2728370.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2759..3088)
                     /locus_tag="PC472_003062"
     CDS             complement(2759..3088)
                     /locus_tag="PC472_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2728371.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3112..4401)
                     /locus_tag="PC472_003063"
     CDS             complement(3112..4401)
                     /locus_tag="PC472_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2728372.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4417..4743)
                     /locus_tag="PC472_003064"
     CDS             complement(4417..4743)
                     /locus_tag="PC472_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2728373.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4878..5507)
                     /locus_tag="PC472_003065"
     CDS             complement(4878..5507)
                     /locus_tag="PC472_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728374.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5722..8379)
                     /locus_tag="PC472_003066"
     CDS             complement(5722..8379)
                     /locus_tag="PC472_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2728375.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8738..9682)
                     /locus_tag="PC472_003067"
     CDS             complement(8738..9682)
                     /locus_tag="PC472_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728376.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9675..10700)
                     /locus_tag="PC472_003068"
     CDS             complement(9675..10700)
                     /locus_tag="PC472_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728377.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKAEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10775..12343)
                     /locus_tag="PC472_003069"
     CDS             complement(10775..12343)
                     /locus_tag="PC472_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2728378.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12456..13367)
                     /locus_tag="PC472_003070"
     CDS             complement(12456..13367)
                     /locus_tag="PC472_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728379.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13351..14337)
                     /locus_tag="PC472_003071"
     CDS             complement(13351..14337)
                     /locus_tag="PC472_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728380.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14697..15098
                     /locus_tag="PC472_003072"
     CDS             14697..15098
                     /locus_tag="PC472_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2728381.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15101..15499
                     /locus_tag="PC472_003073"
     CDS             15101..15499
                     /locus_tag="PC472_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2728382.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4939 a         2735 c         1736 g         6260 t
ORIGIN      
        1 aaaatacaaa ttatatttta ttaattttta ctacttgtaa attattatct tatacaatga
       61 atttgtatta tataataagt atttttagga ttctgttaca taatattatt taaatattct
      121 tctatatttc tcttcttctt cttcagtaaa cacattaaat acaactttta aatttttttc
      181 aggattttct tttagccata gtctaactct acttacagca agattagctg cctctttttt
      241 tggataacca aatacacctg tagaaattcc acaaaaaaca atatttttta tttcatcaat
      301 ttcttttatt gtatttaaac atgatttata acaatgaagc agttgctttt cttgctcttt
      361 acttggctga ccaccagata caataggtcc aacagtatgt actacaaatt ttgcaggtaa
      421 acaataacct ctagttatct tagcatctcc agtatattct aaatgccctt gtttttttat
      481 tattttgtca caatcttctc taagtctagg acctgcgcat gaatgtatct cattatccac
      541 gcatagatgt aatggttgta aacaccctaa taacttgtta tttgcagcat ttactattgc
      601 atctgctctt aaattcgtta tattacctct ccatatggca atcccttcct ctacttcttc
      661 tatatcattt acatctacta actctctttc ttcacattca atcaacaata aattatgtaa
      721 catattaagt atctctggag caatttcttt tggtggatat acattcaaaa gtcctctaag
      781 tatatctcta ggcttaattt catcatttga taaattgtat attttagaag gaacttcttt
      841 tgaaaaatat gcaattaaag tgtttatatt ttttcttctt tgttcgtcag ttagaggctt
      901 gatagtttta tcaaaatcct caaataaatt tacatcatca gcataatctc tccacttcat
      961 agccatctct ccattcttgt attttagact taaaaataat tatgtattta tgtacatatt
     1021 tatttatttc tataaacacc tacataaatc ctgttaaaag tgtaaatttt ttattatgca
     1081 acttctctgg ttatttctat tgattgatag taaattattg tacctaaatt taatccaact
     1141 aacaagtaac taaacctact ttcctaactg caaaatacac tatgtttgcc acttgtatct
     1201 catttgtaaa taaataaagt ccctacaaat acattaacta ggctatgtac taaactgtag
     1261 gaactttaaa aatatattat tcttaattaa gttttggcca ataattttta tttgcttcta
     1321 ttaaatcatc tagtaattct tttgcaactg ttgcactagg aactgtctta gatagcataa
     1381 gtgcttgcca caatttatta taagacttct caatccaagc atcaactact aatttttcaa
     1441 ctgctaattg ttgttctatt aaaccttttt gaaatgttgg tattttccct attgataatg
     1501 gttcataccc gtcttttcca actatacatg gaatttcaac catagcatca tcttgtatat
     1561 tttctatagc tccattattt tgaactatca ataacatacg ctcctgtgta ttaaaagcta
     1621 gagctgtagc taaatccact ataaactctg catgtattcc tatcttaagc ccactgccct
     1681 tagaatgtcc ttgcttttct atcaatgcac atgaaccaaa aacttctttt tctcttccat
     1741 caataacttc attggcacga gtataattta catcagaatg atttacaaca taatctggaa
     1801 atagatagta ctttaagtaa ctacttggta ttgtagttaa atctgttttt actatttctt
     1861 tagtaaataa attagtttca aaccaactat ctttcttgtc atttttcatt ccttcttcac
     1921 catttataaa accatattct tttatatgtt ctactaattt tggtaaaagt tcatttcctt
     1981 ttttatcttt aattgaagta taccaaccaa agtgatttaa tccaaagtat cttatatcca
     2041 tatgtttacg agatttgagt cctgcaatcc ttgctatatt atgttccata cctattggca
     2101 tatcacaaat atttattacc ctagaattag gtctcaacct tctaacagct tccgctatta
     2161 ttgatgctgg attagaataa tttaacatcc aacaatttgg agaatacttt tccatataat
     2221 ctatgttttc aagaacacct tctatagacc tcattccata agctattcca cctggtccac
     2281 aagtttcttg accaactacc ccatgtttta gaggtatctt ttcatctagt tctctcattt
     2341 caagttttcc aactcttata tgagctaaac aaaaatctac atctgtataa gcctctttag
     2401 ggcatgttgt tgctaaatac tctatattag ggtccttttc ctttattagt atttcacaag
     2461 ctttagctac cttattttgt ctttcttcat cattatcata taatttgatt gctcttaatg
     2521 gtaacctatc taagttatct agcaacatta atatgatttc tggggtatat gtacttcctc
     2581 ctccagctat tactactgaa aatttttcca taataatatc tccttttcta tttaaaaatt
     2641 tactgtattt ataaataaat ttataaatac tagtacttta aagtaaaagg gagttcattt
     2701 attaaatcta ttgatttaat aaactatagc caaacttcaa gcactattta caagaaattt
     2761 aattcttatt aattcttggg tccatcatct caaatatatc tacaagttca gatactaaag
     2821 aatgagcaaa taatgcattt gaataatggt cttcagagtg cacaactaaa aaatcagtcc
     2881 cactatcttc actattcata gcatttgata gtaattctaa atgatgcttt gatgctgttt
     2941 gtattttctc tgaggctgaa tttaaaaggt gttttgcttt attgtagtca ccttttttta
     3001 tttccttaag agacatcatt acatcacttt tagcatctcc tgcttctgat attatactaa
     3061 atactttttc ttccatttca ggtaccatat ccttctcctt ctaatgactt gttaaaaatc
     3121 aaagctatca tcatctaatg ttgtagctaa ttctgcattt ttatttaatt cttccttctc
     3181 cattatctta aagaatggat aatatatcaa ggtcataagt attatacttg caaatactaa
     3241 tacgattgcc ttccaatcta atgtcatcaa aaatgctgca acccctggag gtaaaaagcc
     3301 tggtggttct gcaataggta atcctactaa tcctactttt aatgccataa aattaagtgc
     3361 tccaaccaat acaggtccaa atacaaatgg tacaaacata tatgggttca taactattgg
     3421 tgttccaaat attactggct catttattcc aaatagtgat ggtaaaatca caacttttcc
     3481 catactatta aaactcttac acttacttct tgacataagg tacactaatc ccaaagttat
     3541 ccctgaacca gttacttgca agaaaccata gtaaaaacct attgtaaaca catattttgg
     3601 gtctcctcct gctgctatca gtgttgcatt ttctgccata tatgtagctg ctattggcat
     3661 ccatatactt gaagttattg ctgctccatg aagcccaaag aaccataata attgagttat
     3721 aaatagtatt atagttactg caaaaacatt atctacactg cttatagctg gtgctaagaa
     3781 attcattata atagctggta gtggttgacc tgagacattt ttagttattg ctgctattgc
     3841 tgtaaataac attatacata ctatcattgg agttaagttt ctaaaacttt tatttactac
     3901 actaggtact tcttttggaa atctaattac gagattttta tcattcataa acttagtaat
     3961 atcaactact aaaagtgcta ctaatatact tacaaataaa ccttgtgcat ctaaataact
     4021 tgtatctaat acaccatcta ctacttgccc acatacaaca agttgtgcaa caactgctgt
     4081 catacctatt tctacatgat taagattata ttttttagct agtgcctgag ctataaaaaa
     4141 tgctgaatat atagataata aactcatagt aaatttatta gggataagta atgtatccat
     4201 attttcagat aataatgttt ttgcacttcc ttcaggtaac atattgttta atgccataaa
     4261 taacatactt aaagaaccaa caattattat tggggtaatg cttatcatac cttcttttat
     4321 agcttgaaga tgcctttgtt tttcaatctt ttgtgctatt ggaagaagaa atcttcttaa
     4381 attattttga agtgcttcca tttttattcc ccccaattat ctatttttat ataattcgat
     4441 tgccgacttc ataacagaag gagcatccat acgaccataa tgcttagtat ctattaaacc
     4501 aactgctatt ttgtaattct tacaagcttc aactacatca tcttccttaa acctaatttg
     4561 tggtgctaca agtactacat ctgtatcatt agcttcaatg tatgatttta aattttcaaa
     4621 tgatgttgcc tctatattta tagttttagt ttttaacttt tcatgctcac tacaaaaact
     4681 tttcatcttt tccacaagta cacctgttga agctccattt gcacatacta ataaaatatt
     4741 cataattacc ctcctacaat atctaacctc ttttgttttc taatttttta tcagccaatt
     4801 cttactctaa ttcctatcag tttttacttt aattcttatt attttatttt aattcttatt
     4861 aatatttact ctaattctta gtactgccaa tgattaaact tgaaagccgt atctataact
     4921 tcttcttgct tatatttttc taatattttt aagtatcttt ctttataatc attatctact
     4981 tctttttctg gtattactat actagcttca tttaaaaagt ggaatgaatc ccttcccatt
     5041 tctaatcctc tatatgtatg cttgtcaagt gccacgtcca taacttctgg aactttaccc
     5101 attgcaaatc cttttataat tatatttttt aataaatcac ttgatctatc tttctcacat
     5161 gcacaaagat atcttattgc atgtatgaaa aataaaggtc tgtctccatc tgcatatgaa
     5221 tattcctttc ttacttgatt taaattattt atgattatta cagctgatgg gtcccccatt
     5281 cctatatctt caactgatat tgtaagtatc cttctccata gcttatcttc taattctggt
     5341 gatgatatat atagttcata agcaaattca caagcttctt ccaccatatt tcttcttata
     5401 gctttttgaa atgacgatat tatttcgtct gcactaaatc cgtttcttgt agtcattctt
     5461 gcccatgggt catttgcctt tttatctctt aatattttat cttgcatatt atttactcct
     5521 ttttatttaa attttaatta tttatattgc tcatttaata atataagcaa gtattatgcc
     5581 agttttttta gacactttaa aacactgtta tttcaatcat ttttatcatc aaaacttaga
     5641 tactttgtct aagtatattt atatgtgtct aatattaata ttattttaga ctaaaaaaaa
     5701 ttaccaaata agtttttata ctcattggta atctttttcg attatttctg catattctaa
     5761 tattaataat atttgatagc actctttttc attaatttct atattatatt ttttatctaa
     5821 ttgagatact atatctttta ttttgcttag actcttgtga taatcaagtg ttttttcttt
     5881 atcaagcttt tcaatttctc tagtatttcc aatcaaccca tctaccatga aagctaaatg
     5941 catcataagt ccaattactt tatcgtattt cttttctata ttaaatacta aaaacaattc
     6001 atcagacaca tattttatat ctttaaatat cctattataa ttagagcttt ttaaggatgt
     6061 ctttaaacct tcaactacat catcataacc tatacttatc tttataatat tttttaattt
     6121 attgatattt tcatccttat ctatatcttt tttgctaata tagtcaattc ctctaatatt
     6181 aattctttgt tcattgatta ctgcaacaat attactactt tctttaagtt tcgtaatatt
     6241 taaacttaaa tctttttctg ttttatatgg gacagatata atatctattc catctaagta
     6301 cctactcatc ttttcttcta atatttttct tgttttatta tctacacctt ctgaaacatg
     6361 tgatgctata ataattgttt tagacagatt ttctttattt tgaatttcat tagttccaaa
     6421 atagtacttt ctcgtgttag tgatagagtt gtatacgtca tttaaaggta atcccatatt
     6481 ggccttctga actgcttcta acacagttaa tgtcgtcacc ataggaatta ctcttactcc
     6541 acactttttt tctttcttta taatttcatc aaaagagttt aatgaaccca tatctgtaaa
     6601 tagtaataca tctttattgt ctatattatt aattaaattt ttaaaatcta caataacttc
     6661 atcatagctt ttatctaact tcatatcgaa agaaatagca ctgcactttc caataatatc
     6721 accaactgct tttaccatac tacttgctgt agtttctcca tgcatagcaa ctataatatt
     6781 aatgttattc actattttat tttgttcatc ttgtttagct tgtactatga ataatgtaat
     6841 atatgctaca gtttctatag ataaaaataa atcaaattca ttttcaatta ttccaactat
     6901 ttttaatgct gttttaaatt cacttctgtt atttcttcta atttgattca aatctatgtt
     6961 gtttgtagag ctacttttat ccaatacgct ttttatataa attgccattg acttaaatat
     7021 tgattcactt atatctattt cttcatcttc tttaataatt tgtcttattg attcacacat
     7081 gcttttaaac ttaacattat taaatagaga agcatctgct atattcattt ctacagattt
     7141 attaagatta gtatttactg cattaataat atttatatca ttgtagttaa taacttcttc
     7201 ttctcctaat gacgtaaata caaaatattc tattccatgt gttataactt catctgcttt
     7261 aatagacttc tctctataat catcactcac ttcatttata gatttatcaa aatatttttt
     7321 ctctatataa actggttctt tattatctct tttatatctt aggtatccat ttgcaacagc
     7381 taattttata tcattttgaa gttgacctac attattttta caatcataaa gtaatagtga
     7441 tttaattatt tctttatgta cttttattga aatagaaata ctttttgatt cttccttgaa
     7501 aaaatttttg actaattcca ttctttcaat cattgtccta tctctcagtg atggtatttc
     7561 tattttcatt ggtattcttc tattgaatgt atctaatagt gttgatgtcg gattttctgt
     7621 agtagcacat attattaata ccttagactc tctttctttc gttgcatctc ctagtctttt
     7681 aaatgttccc ttatctataa aagtaaacaa catttcttga ccttctgctg gtagtctatg
     7741 aatttcatct aaaaatagta tccctccatt tgcaatttcc aaaagacctt ttctggaaga
     7801 ttctgcacct gtataagctc cttgttctac cccaaaaata tgtcccataa gtaattgtgc
     7861 attattagca taatcagcac aattaaatgt tacaaaagga gctttatttt ttctaattcc
     7921 actatctata gaataatcgt acatatatct agcaatcata ctctttccta ctcctgtttc
     7981 tccatagata atagtatgaa gtccatttgt aggatagagc attgcgacct tgcatttttc
     8041 tattataggt cttaaactat gttctttacc tattagtgtt tcaaaactat ctttttttat
     8101 tttgttttct acttttgttc ctttttttat gccaagcaac tcactttgtg tatcaagcaa
     8161 ttcattcttg ttaaggttaa gatttgcagg gatataatac acaggtcttc cttttatctt
     8221 cgtaaccata ccctcattga ataattggtt taaatatgta cttatattgt ttcttgcaat
     8281 tcctgtcttt tctgataatt ggttagctgt aatccctttt tcattttccc tatatgatga
     8341 ttctaaaact ttcattactt tatctttttt atatatcatt acaagctccc ctacattata
     8401 gtattttatt aactaaaacc cctatacaca ctttattttt taataaactc ttattcttta
     8461 actaaggtta taatttatca aactttcctt actttaaatt atagaaaatt attgtagaaa
     8521 aaataagatt attgctcatt ttttctacat tactattttt aattttatag tactttctta
     8581 acaacatact ttacttgttt ttaattttat tatatttgaa tttattatat ttgaaaagta
     8641 gaaataaaaa accctatttt ggagtagttt taaaatttct ccaaaaatag ggttttagtt
     8701 tgtatttcaa ataaatacct gttcaagtat ttttatacta aacaactttt aaattttgtt
     8761 gatatttatt tatttctgat ttagaagaat atacaaaatg tccatctgaa acctcaatcc
     8821 aacttaatga cttttcattt gaacaatcat atccattaga attatactct attctatttc
     8881 ttgacttagc atatctaggg tcaggaacag gaattgcaga aagtagtgat tttgtatatg
     8941 gatgaatagg attattatac aattcatttg aatctgccaa ttctactaat tttccattat
     9001 acataactcc tatgcggtca ctaatatgtc tcaccataga taaatcatga gctataaata
     9061 aacaagttaa attcttttct tgttgcaatt ttttaaataa atttactatt tgtgcttgta
     9121 tagacacatc caaagaagct ataggctcat cagctattat aaattctggg tctactgaaa
     9181 gagctcgcgc tatgccaata cgctgtcttt gtccacctga aaaatcagat ggatacttat
     9241 ttgcataact tctatcaagt cctactagct ttagcatctc atatacttta ttcattctat
     9301 ctgttttatt tttacagata ccttgaattt ttagtggctc tgaaattata tctgcaattg
     9361 tcatacgtgg atttaatgat gaagtagaat cttgaaaaat aatctgcata cttttgttta
     9421 catctttttt tataaatttg tacgtttttt tatctgatat gcaatttcct ttgtaaatta
     9481 cttctccacc agtagattca tgtagtctta ttatagtttt accagttgta gattttcctg
     9541 aacccgactc tcctaccaat ccaaatatct cacccttata tatatcaaaa cttatatcat
     9601 caactgcttt tacagtagta cttttgtcca aatgaaaata ttgctttaag ttttttattt
     9661 ccagtatttt ttctctattc attagaaatc acccttccac aatttactct aactggtaca
     9721 tctacttcag gtgcatctgg gtgcaataac caagttgctg cactatgtgt atcattaatt
     9781 ttaaacattg gtggctcttt ttcataatct atcttaagtg catttttatt ccttattgca
     9841 aaagcatctc cttttggtgg gttcaataaa tttggaggca ttccaggtat attatataaa
     9901 tagtcatcct gcgaatctaa agtaggtaat gaacccaaca acccccaagt atatggatgt
     9961 ctagggtcat aaaatatatc ctctacagtt cctatttcta caatctttcc tgcatacata
    10021 actgctattc tatcagctat agttgcaact actcctaaat catgagttat aaatattatt
    10081 gacaaaccta ttttatgttg taaatccttt attaaatcaa ttatttgagc ttgtatagtt
    10141 acatctaaag cagttgtagg ttcatcagca atcaatacat ctggattaca ggcaagtgct
    10201 atagctatta ctatacgttg tctcattcca cctgaaaaat gatgtggaaa ttgtttaaat
    10261 cttttctcag gattatcaat tccaaccaat tcaataagct ctatggctct cttttttgct
    10321 tcacttttac ttataccttg atgaatgatt acagcttctg caatttgctt tcctattgat
    10381 atagttggat tgagagatgt catagggtct tgaaatatca ttgatatatt ctttcctctt
    10441 atatcttcca tctctttttc agccttctta accaaatcac ttgatttaag taaaacctct
    10501 ccatttttaa tatatccatt gtatgggagt atcctcatca aactcttaca cagtacagat
    10561 ttcccacaac cagattctcc aactatagcc aatgtctcac ctttttttag attaaaagat
    10621 acatttctta cagcctgaac ttctccttct tctactttaa aacttacaga tagattatta
    10681 acctccaata aatgttccaa aaaccatcaa cccctatgct attcaaacct tagggatata
    10741 tttttatccc taaggtttct aattaatttc agtattattc aattgtccag tcagctatgt
    10801 tccagaatat acctacacca tgatgaccta aaactgtatc aggtgttaat ccttttatgt
    10861 ttggttttcc aacatatatt gcatctatat aagcaataaa tgtataaggc atatccttag
    10921 tcatttctac ttggaattgt ttatataact ttaatttttc atctttatct tctgtttctc
    10981 ttgctttttg aagtatttta tctatagtag gattagagta agcactgtaa tttgcacctt
    11041 tatctgttcc aaatacttta tatgtatggt catctgggtc aaatggactt ccccaaccta
    11101 ttaaatgagc atcttgatta gcccaatctg tttcagttac aacaacagct ttagcatcaa
    11161 caccaatttc ttttaactgt tgtgcacata ttttagccat atcaactcta acttggtcac
    11221 tctcacctgc tgttatctcg aaagctaact tagtaccttc tttttcatag attccatcag
    11281 aacctaattt ccaacctagc ttttcaattt cttgtttagc tttttctgga ttatattcaa
    11341 acttttctat atcaggatta ttatatggtc ccatttgtaa aggtgaatat gctggtacac
    11401 catgtcctaa taatacactg tcaactattg cttttctatc tattgcatag cttaaagcat
    11461 ttggtaatcc tttagctttt ttatctttaa agaatttaga attaaaattg tataatatac
    11521 ctctatagtc ggctgttttc attatattta ctttaaaatt cttttcgtct ttttcaaagt
    11581 tagacatatc tttaggagtt atttgagcta ggtctaattc tcctgatttt aattgcattg
    11641 ccttagcctt atcatctgga acaatcttaa agactacttt atctaatcct ggttctttta
    11701 caaaataatc actattctta acaagtgtta tactttgacc tttgtcccat ttttcaagtt
    11761 taaatggtcc agtacctatt ggtttttgat taaattcatc agtagctata tccttacctt
    11821 ccaaagcatg ttttggtaat actcctactg ttaaataatc aagcattgct gtatttggag
    11881 cttttaaagt aatttttata gtattatcat taaccacatc aatttttgtt atatcttcat
    11941 aatttgatgc tatctcagaa gcattatctg gattcattat agtttctatt gtaaacttaa
    12001 catcatttgc tgtaaatttt tctccatcat gccacttaac atcatctctt aaattaaaag
    12061 tatatgtatt tgttgcctca tcaaatttcc agtcctttgc tagacaagga accaccttat
    12121 tattttcatc atgagctgtt aatccattaa atattaacga atttatttct ccatgttcat
    12181 atagtgcagg atttatactt gtatagtcat tacttccata aacaagtacc tttccatctt
    12241 taggcgtatc tgccttctta tctttgtctc ctccactaga acaacctgcc attagactaa
    12301 gtatcattac taaacttaaa acttttaact tctttaattt catgatgctc ccccttttgc
    12361 tttttataaa atatttttca aagctttgac cccatttatt taaatagata tttcaaatac
    12421 ctcagtattt gcttatttaa tactcaaatg ctgatttata aattactaga ttttttatta
    12481 ttattctttc ttatataatt acctatattt gttatacaca ccaatgtggt aaccaaaaat
    12541 atacccggaa tcagtataat ccaccatcta tttgaaagta atgcttcctc tgacaaagat
    12601 aacatacttc cccaagaaac aatttctatt ggtaaaccaa ttcctaaaaa gctaagtgtc
    12661 gattcagtac caatagctgc gcctatgttt gttacaacca tgaacattat tgatgccaca
    12721 aaatttggaa gtaaatgttg ctttaataca tagaagaatc ctcctcccat gctcttagct
    12781 gctagtatgt attcgctatt tcttatttgt ctaacttctg ttctcactat cttagatata
    12841 ttcatccaac ttgttatccc tattactatg gacatagata ctggatttga atttccaagt
    12901 atagcttgta caaatattat aattaatatt gaaggtatac taagtatgat ttctgtaaac
    12961 ctcatcattg catcatctac gagttcagat gctgaaccac ttatactacc ataaacaatt
    13021 cctataacag ttgatattac agtggaaaat agtcctataa ataaagatat ttttcctcca
    13081 taccatatca tagaatatat atctctaccc attgagtctg ttccaaataa aaaagtttta
    13141 tttggagcta aattagaact tgctaaatcc atgtatgttg gttcatgtgt cattataaaa
    13201 gatgagaata cacttccaat gactatgatt gataaaataa taattgatat atatggtaat
    13261 tctttaattt ttttagataa ggttttcttt gtttctatat gttcatcttc tgctactaaa
    13321 atataatttt ctcctacaat ttcaaaatca ctatattgtg gcttcattag tatcacctct
    13381 atcatatttc attctagggt ctattttatt gtttataatt tgagctaaca tattcccaaa
    13441 tactactaaa gcccctgtta ttaagcataa aactaataac atattataat catgatactt
    13501 tgcactttca aaacaaagtg ttcctagccc aggataagaa aaaacttttt caactacata
    13561 agttcctcct agtatatgag gtatcgaaat ggccattata ctaatatatg atggcattat
    13621 atttctcagg caatgcttaa aaacaatagt tctattattt aaaccttttg ccttacataa
    13681 aagcacataa tcttctctta tttcctctag taatttattt cttatcatat atgtgtaata
    13741 ccatagatgg cttaatataa gtactgtaag aggtaagatt agatgtgata tcctacttaa
    13801 tatactggac tcttctccca ttgaataagc accactactt ggaagtatac ttaagtttat
    13861 actaaatata agtatgagta ctaaggcaac ccaaaatgat ggaatacagt tggttattgt
    13921 tcctaatttg catatgattc tatcaatgag cttatcttca tgtaatgtac aaaatattcc
    13981 taacaataag gctagcacaa aggtaagtat atatcctgaa ccacctaata ttatagtgtt
    14041 tatatagaca tcatttataa cagaagtaac attttgttta tatttaaatg aaattccaaa
    14101 ttcccctttt gatgcatttg aaatccattt tatatattga ctgtatattg gttcatccaa
    14161 accaagtttt ttcatagcat tttctttttc ttgtgtactc attctttcta ctccatcccc
    14221 atagtaagag attaatgggt ctcctggagc aagtcttgcc atgtaaaaaa caataaaaga
    14281 taataaaaac attactaaaa taaactgcaa caactttttc agtatatcct ttaacaaacc
    14341 ccaacccccc tttgttgcaa taaaaaaaac cacataaaag caaacgtaaa gtaacgttaa
    14401 aaccttcgtg gttccattat cattaaacaa aattaaattt tctatattta tctatgagtt
    14461 aattttagca tcatttatta actgtgtaaa caaacttttt aaaaattgtt aaaatttcat
    14521 caaattattt tatttatacc taaaataaaa tttttatatt ctattcttca ttattttcta
    14581 taaaattata aaattttagg ttgactatat aagaaaagta tattatatta gtttgtatgc
    14641 aaaccattta agaggtgatt ttattgaaaa gtaattatga ctgttttaga atagccatgc
    14701 tcttaaaaga actttattct aaaactatgt acacagtaga agaaaatttt aaagaaaatg
    14761 gattaacaca ccaacagatt atagttataa aactgatagc tcataatcaa gaacttacaa
    14821 tatcacaact atgtgatgaa atgtctttag ctaaaggtac ggtttcaggc ataatcagca
    14881 gattagaaca gattggatat atagaaaaat tcaagaaatc taatgataaa agaaatacat
    14941 acgttaagtt tacaacaaca ggatttgaat ttgccacaaa ttttaaaatt aaaatgcaag
    15001 aaagttttga tgatattttt aaaaattgtg atgaaaacga attgagtgac ttagtaaaaa
    15061 atcttagaaa tatattggca aaagtaaagg agagataatt atgaattata aattaatact
    15121 tgcaatagta tttataacat cagctttaat tttctacact ataggtgtat ttggagaaag
    15181 aaaggcaaaa attttaaaaa agaaacatgt aattatattt tggcttggtt ttatatttga
    15241 tactttagga acatttacaa tgagtaacat agccaacagc catacttttg aagtaaaatc
    15301 tgctttatca caaaaccttc atagtattac aggtcttctt gcaattgtgc ttatgctttt
    15361 tcatgcaagt tgggctactt ttgtcctata caaagatgat gaggaaaaga aaaaattctt
    15421 tcataagttt agtatagttg tatggactat atggttagtt ccatatttta taggtatgtt
    15481 tattggtatg gctggatagt aaaataaaaa gtgtgaaaga aggatgtagg taaatttgta
    15541 taagacaaat ttacctacat ccttctttca cacttttaca tatttattta taatactcca
    15601 ttatttcttt catgttcctt aatacctata tagaatattt aattgtttct atagtatttt
    15661 attattttct
//
LOCUS       DAMJYC010000092        15623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJYC010000092 DAMJYC010000000
VERSION     DAMJYC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.094"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            163..1554
                     /locus_tag="PC472_003074"
     CDS             163..1554
                     /locus_tag="PC472_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2728383.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1808..3601
                     /locus_tag="PC472_003075"
     CDS             1808..3601
                     /locus_tag="PC472_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2728384.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3682..5064)
                     /locus_tag="PC472_003076"
     CDS             complement(3682..5064)
                     /locus_tag="PC472_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2728385.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5322..5669
                     /locus_tag="PC472_003077"
     CDS             5322..5669
                     /locus_tag="PC472_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2728386.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5687..6721
                     /locus_tag="PC472_003078"
     CDS             5687..6721
                     /locus_tag="PC472_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728387.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6770..7819)
                     /locus_tag="PC472_003079"
     CDS             complement(6770..7819)
                     /locus_tag="PC472_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2728388.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8094..9629
                     /locus_tag="PC472_003080"
     CDS             8094..9629
                     /locus_tag="PC472_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728389.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9814..10500)
                     /locus_tag="PC472_003081"
     CDS             complement(9814..10500)
                     /locus_tag="PC472_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2728390.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10625..12007
                     /locus_tag="PC472_003082"
     CDS             10625..12007
                     /locus_tag="PC472_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2728391.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12409..13677
                     /gene="eam"
                     /locus_tag="PC472_003083"
     CDS             12409..13677
                     /gene="eam"
                     /locus_tag="PC472_003083"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2728392.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13770..14126)
                     /locus_tag="PC472_003084"
     CDS             complement(13770..14126)
                     /locus_tag="PC472_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728393.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTFEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14277..15371)
                     /locus_tag="PC472_003085"
     CDS             complement(14277..15371)
                     /locus_tag="PC472_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2728394.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         6054 a         1927 c         2507 g         5135 t
ORIGIN      
        1 ataatatttt tcataatttt aaaaataata ataaaaaaat gaaaatacag gcgataatct
       61 attgagtaaa catagaaaag atgatattat aagtatgtgc taaaatggtt aaagcaatat
      121 taatattaaa tttaactata aaacaaaagg gggcgttttt ttatgtcaag tacagaaagc
      181 gtatctaaaa aaagtggtca tccgaagagt ttttggctta tttgcatgac aattttgtgg
      241 gaaagattta gttattatgg tttaacagct ttattggtat tattttttac agcaagtgta
      301 gctcaaggtg gtttaggtct ttctactcaa gaagcaacat ccatgtttgg cttatttaca
      361 ggtcttatat atcttacacc attagtcggt ggctggttag cagataaagt tttaggtcaa
      421 caaaaatgta tttctatagg tgcactttta atagcaatag gagatttcgt actatttgct
      481 tcatctacat ctattgcaat ggtttgggta ggtctatcaa taattatttt aggaaatggt
      541 ttttttaaat caagttgctc aaatatagtt ggggatatat atcctaaaac tgatgtgtca
      601 agaaaagatg cagcatacag tttattctat atggcagtaa atgtaggagc atttatagct
      661 ccaataatat gtggattgct ttctgataat gtatttgcag taagaggagc aagtggagat
      721 atagtatctt atggatataa gatggcattt ttaatttgtg gtataggcat gatgttaggt
      781 atgattatat ttacacttct tgcaccaaaa ttattaaatg aagtcggaaa atatcctgtg
      841 actaaaggta ataaaggtga aaaaattgag tatggaccac ttactaagtt ggagaaaaat
      901 aggataatag caatggttat tttattttta tttgtggtct tattctggac agcatactat
      961 caaacacaaa gttcttttat gatttatgta agggataatg taaatagaac tctatttgga
     1021 tttgaaatgc cagtagcatg gcttacatca ctaaatgcta tattatgtgt ggctttatgc
     1081 ccagtattag ctaccctatg ggttaaatta tctcatacca aaagaggtga tttctctatt
     1141 ccagttaaaa tgggattagg aatgataata atgggtgttg ggtttatagt tatggtattt
     1201 gctacattag ctaatggtcc agatggaatg gtaaaggcta gtatattgtt tatagtattg
     1261 acttatatat ttacaacagt aggtgaatta ttcttatctc ctataggact tgcaatgttc
     1321 aataaattag ctccagctaa gtatgctacg ttgtctatgg gagcctggta tctaacaagt
     1381 ttttttgcaa gtttattatc aggaaagatt gctggtttta cagccacact tggtttttta
     1441 cagatatttg gaggaatagc aacagtttta ataatatttg gtttaatatt attgggaata
     1501 agaaatgtat taacaagatt aatggctatg gatttactaa caaaagaaaa ataattcatg
     1561 acgaattatt gtaaataaat gaattacttt acaataaagg aagttacatt taaatataaa
     1621 attaaatgta atttcctctt tgattatgaa aaaaagcttt acacaaatag atattataat
     1681 taaaattata attgtgataa ttagagtgta taagatttat ataaattata caaaaaatat
     1741 ttatgtaata aactttacat acttatatat ttagttattc aaactattat ttcgaggagg
     1801 tcttgttgtg aatataaaag atagattaag tggattaaga aaatttatgg aagaaaaaaa
     1861 tatagatgcc tatatgattc catcttctga taatcaccaa agtgaatatg taggagatta
     1921 cttcaaatca agagaattta tatcagggtt taatggttca gcagggacgg ttattgtgac
     1981 taaagacgaa gctgggttat ggacagatgg aagatatttt atacaggcag aatctcaatt
     2041 agaaggaagt acaataaaat tattcaaaat gggacaagaa ggttgcccaa ctacagatga
     2101 atatttatat aaaaatattc ctgaaggagg aactttagga tttgatggta gagttatatc
     2161 tgctagagaa ggagctacat tagctgaaaa attatctaaa aaaggtataa agatagaata
     2221 tcagtatgat ttaatagata gtatatggcc agatagacct gctttatcag atagtaaagc
     2281 atttttatta gatgttaaat attgtggaga aagttttagc tcaaaattag ctagattaag
     2341 agaaaaaatg agtgaaaaag gtacttctac tcatgtaata actactcttg atgatatagc
     2401 atggttattc aatataagag gtggagatgt taaatataat ccagttgtac tttcttatgc
     2461 agtaataact ttaaaagaag tttatctatt tgttgatgaa agtaagttaa atgaagaaat
     2521 actaaatgaa ctagctaagg aaaatgttca aataaagcca tataatgatg tgtatgaatt
     2581 tgtaaaaaat atagacaaaa ctgaaaaagt tttattagat ggaactaagc ttagttatac
     2641 aatttacaat aatatacctt gtgaagttga gaaagttgat gagttcaatc cagtaatgtt
     2701 ttttaaagct caaaaaaatg aggtagaatt agaaaatata agaaattcac atgtaaaaga
     2761 tggtgtagct ttcactaaat ttatgtactg gttaaagaaa aatgtaggta agatggaaat
     2821 cactgaaata agtgctactc aaaaattaga ggacttaaga agagagcaag aaggtttctt
     2881 tgagccaagt ttcaatacaa ttgctgcata taaagagcat gctgcaatga tgcactatag
     2941 tgcaactcca gagtcaaatt ataaattaga ggcagaagga ttatttttag tagattcagg
     3001 tggacaatat tatgatggaa ctactgatat aactaggact acagttttag gacctataag
     3061 tgatgaatta aaactacact ttacttctgt agcaagagga atgataaatc tttctaaggc
     3121 taagttcttg catggttgta gaggatataa cttagacata ctatctagaa gttgcatgtg
     3181 gaatatggga attgattatc aatgtggaac tggacatggt ataggatttg tgctaaatgt
     3241 tcacgaagct ccaaatggat ttagatggag agtagtacct gaaagatttg atagtgcagt
     3301 tcttgaagaa ggaatggtaa ctactaatga accaggtata tatatagaag gttctcatgg
     3361 tataagaaca gagaatgaaa ttgtagttag aaaagctgaa aagaatttct atggtcaatt
     3421 tatggaattt gaagttgtta cgttagctcc tattgactta gatggtatag caccagaatt
     3481 aatgaataag gatgaaaaag attatttaaa ttggtatcat aagttagttt atgataaaat
     3541 atcacctttc ttaactgatg aagaaagaga atggttaaaa gtttacacta gagctatata
     3601 gtttgtacta gatacaataa aaatcgaggt attataatgc ctcgattttt tattgtatat
     3661 tttattaact ttaaaaaatt tttaatttga taattctact aattttttat tcattataag
     3721 tagtataatt ccaaatatca ataaaaatga tggtatcact atgaagactt gtaacatacc
     3781 aagttggtct ataatagctt gcgtatagcc agataattta tttgctgcaa atttgcctaa
     3841 aaaccaaaca cccattagga atgaaatata cttttcaggc gaaagtttat ttaccatacc
     3901 atacccgact ggagaaaagc aaatttctcc tatactttgg aatgttaaaa atccaactac
     3961 ccaaaataca cttgctttgt gaattgaatt agggtctgtt cctgtttgta taactgctac
     4021 tatcataaac aaaaatgcta tggctaatag tataaaacct aagctcattt tttttggcac
     4081 acttaaatcg ccttttttag tttgagataa ttttacccat actaaagcag agataggtcc
     4141 aagaataaca caaaggagac cattgtatga atctatccat gaagtaggaa tttgaaaatt
     4201 gccaacagta agatttatgt gttcctttgt atagagtgca atagacatag aggcttggtt
     4261 atatgctatc caaaaaaata ctgtgaaaaa agctaacacc aatattacta ttattctttt
     4321 tttctcaatc aaagttaaag gtcttgaagc gagcattttc ttatattcta gtgattttct
     4381 tatagactct ctcccgttaa tagggtcatt attcaaaggt ttataaaact ttctgtttcc
     4441 aaatataaag aagaataaac ctattagaac cgtaattgat gaaaataaaa atacatatct
     4501 atatccatac atacttatag ataatccgct tttcatagca aaccattcat tagcaactaa
     4561 accgcatatt aaagggccaa ataaagtacc tagattagta gccatatacg taatagagaa
     4621 agctccatct tttcttgggt catcattggt atataagttt ccaaccatag tatgtaagtt
     4681 tcctttataa aaacctattc ctaatccaga taaaaatatc atgatgttta tatccatctt
     4741 actatttgct atgtatccta gaaaataacc aattgaaacc attagcattc caagtataat
     4801 acagtttttg ggacctaaaa atctatcact gacccatcca ccaataactg gagagaaata
     4861 agtccatgct aaaaaagatg caagcattga tgcggattct gctggtgtta accctaaacc
     4921 acctttggag acttgttcag ctaaaaacaa taataatacc aatttcatac catagtatgc
     4981 aagtcctaca aaggtaaata ttgaacagca gaaatataaa tctctaggtt gtttaagttt
     5041 ttcttgcgtt tttaaaactg acatatgtcg agaacctcca gataaatagt ttaacaaaat
     5101 tttaccatac tatatgagta tatgtttttt tcatatagac gtcaacttgt aagataattt
     5161 aattaagtcg aaatatatgt aaaaatagta tcatttttgt gtattcagta atattttttt
     5221 atgataatat atgttattat tatattaaaa tacaaacata tttatggaaa ggatatgcca
     5281 atttagtagt tagattgatt gtttggcagg tgggatgagc tatgataaga aagaaatctg
     5341 aattacaagt agaagtaaaa gaaaatttaa atggtggtat aggaaaggtt aagcttgaaa
     5401 acataataca aaatgaagag cttaaaggaa aaggaagact atttaaaaga gtagctttac
     5461 cagtaggaag ttcaattggt gtgcatgacc atacaacaga ttttgaagta tattatatac
     5521 taaagggaaa aggtaaagta tttgataatg gggaatttgt agaagtaaat gagggtgatg
     5581 tagtgtatac agctgatggc gaaaaacatt ctatagaaaa tataggagaa gaagatcttg
     5641 agtttgtagc actagtttta tatatgtaaa atatggagga ggagtcatga caggcaagat
     5701 agaagaaatt aaggacaaga taaatagatt ttgcaactcc aatttaaatc aagaatacaa
     5761 agatataagc tttaaaattt tgcaaaacct tttagataat aataaagaga taattcattc
     5821 ttctagagct gatatatggt cttcagctat actaaatata gttttagaac aaaatctttt
     5881 atacaataag aagcatccac tacatataac aaaaaaagag ttttcaaaac aaataggtgt
     5941 ttctttaaat actataaata ataaatcgtc tttaataaaa gatgtttgta gtattgactt
     6001 tttaaatcaa gatactatag ctatttctaa tgtagaattt tgggtaaaag agactaattc
     6061 caaatcaaag tctgtagatt tagatttaga aaagaagaaa atactatata aaagatatat
     6121 taagctatca caagatgcga atagtgatat tgaatcaatt agatatatgg aggaagctgt
     6181 aaatataggt aaaagtatga ttactaaaga agataaagaa cttggatttt ggaaaggttt
     6241 atctaccaga gcctatatga tagcacttga atcccttgct agaaagttag aaagtattaa
     6301 taatctaaag gagtccaaaa aagtattaga atatttaata gaaattaatc ctagtgatga
     6361 gcagggaata agatacaaat tattaaatgt attaattaga ttaaatgata gaacttcaat
     6421 aaataaatta tttgaacttt ataaagaaga aaaatctgct acatggatgt acagtaaagc
     6481 tctttattac tttaaaaata aaagtatgtt tttggcaaat gattctatta aggttgctag
     6541 aagtaaaaat aagtacgttg gattatattt gttagattgg cgaaatgctt ttggtagaga
     6601 atttgttaca gaggaagaaa aggcagaagc tgtctattac tatgatgaaa acatagtgat
     6661 ttggaatgaa gttaaaggtt ctatggattg gctattaaaa aaaatgttgg aattttcata
     6721 aaaaaaacta ctccttactt ttatataagt caaaggagta gtttttaatt tatacatcta
     6781 gttttaaatc ttcaggtttt attagacctt tttttctcat aaattctgaa atcaacaaag
     6841 ttacaatagc aggaagtata aaattaagta aaagtatacc aacccacata gatatacctc
     6901 cttttccagc actttgcata gcagttactg ttccaaattg tccaaccaat ccacaagtac
     6961 ccataccaga accaatagga atattctcca ttttgaatat tgttgtagat aatggtccaa
     7021 gtaatattga agctacagta ggtggtatcc aaattttcca attttttatt atattaggta
     7081 cttgcaacat agatgtccca agaccttgtg ccacaaggcc accaactcca ttttctctaa
     7141 agctcataac agcaaagcct accatttggc aacaacaacc tacagttgct gcaccaccag
     7201 caagaccacc taaacttaac atcatacaaa tagctgcact acttataggt aatgtaagag
     7261 ctattccaac aagtgcagag actaaagctc ccattattat aggttgcata gttgtagctt
     7321 gcattattat atttccaaat gaagtcataa aagcagaaac agctggacca ataagttcag
     7381 ctacaagtac tccaattagt attgttacag caggtgtaac caatatatct atttttgttt
     7441 cttttgaaac taattttcca acctctacag caaaaagtgt agcgacaaat gctccaacag
     7501 gtcctcctaa ttcattacca caagctccaa caagtgcaca agaaaatagt acaagtggag
     7561 gtgcttgaag tccatacgca atagccactg ctatagctgg accagtaacc tgtttagcaa
     7621 gagggcttat aacttcagta aaaagagaaa tattgaaatt atctcctaat gtattgaaaa
     7681 ttgttccaat taaaagggac gaaaaaagac cgagtgccat ataacttagt gcgtcaataa
     7741 gatacctttg aacagaaatt tcaatatttt ttcttttagc aaatgatttg aaatttcttt
     7801 ttttataatt gatttccaat ttaaaatcct cctcatataa tataagtata atttcttttt
     7861 aattaattat ataacttttc agctgacaag acaaatgtta tttcactaaa attagactat
     7921 tgtaatagta agaaattaac attaatttta gttttaaatt ttaaatttat ggtttataat
     7981 tagtataggg atattctgaa atataataat tttccagttt atcccaatat ttaacttagg
     8041 tagattgctt acaaaacaaa tgattatata ttaggctaaa aggaagtgga tagatgatat
     8101 ttgtaaaaaa taagaaatac agaaaaattg ccataattat atctattcct atattgcttg
     8161 ttgcgcagta ctaccttttt aaatttggaa tacttagacc aatggtaaag ggagtagaaa
     8221 ttgaaattgt agatggggaa tatgttaaag atatagataa atatgtagta aaattaaatg
     8281 aaacagttac tttgtctaca ggtgaatata ttaaggttcc ttcgtatgct aaaaatccaa
     8341 atatttggtt taatgtattg gataactcag ggactttaaa aatagatgga aataaaatga
     8401 cagcattaaa ggttgggtat tcttctgtgg gtatcatgaa aaattcaaga gtattaaaaa
     8461 aggctactat aaaagtagta gacccagagg tagaatcact agatatggag attaatgggg
     8521 atttaaaaca tgttggtgat agtgctacta tagaaagtac aatagaagtt gattatgata
     8581 agtttaaaaa atcatataag ccagtttata agagtagcaa tgaaaatatt ttaaaaatca
     8641 aaggaaataa agtaagtgca gttggagttg gtaaagctac aatatatgct aagtgtggaa
     8701 ataaagaaat tgagagaaca tttaggattc aagctagagt tgctgaaata aatatggatg
     8761 atataaaaat agaagttgac cagaatgaaa aattgaaacc agatataata acttctccgc
     8821 ctggtttaga accaccaaaa atatattatg agttagtaga ttcaaaatta cctgtagaaa
     8881 gagcaataag tttaagtagt aatggaaatg ttgtagggct tagagaaggt aaagataggg
     8941 tgagaataac ttgtggagat aaatctaaag taataacagt aacagtagtc aaagaaacta
     9001 taacgaatga ctatatacaa aacttggttg gttcacataa aatagtaaac aataaagtta
     9061 ttattaaact tgaatgggat tatatgaaag atgtctttga ttatgatata tatcttaaag
     9121 ataagttgtc aggagaatca tttaagaaaa ttaaatctat aactataaat gaaagtgatt
     9181 taggtaagtc taacaaggta tatgcaacta tagaagtaga tatgaagggt aatcaaaata
     9241 tagattttga tatttatgtc atagggaaaa aggattcaga tacaactaag ccaagtaatg
     9301 taataaatat taccaatgga aatggaaatt caaataatga taataatatt aaagatatga
     9361 gggtagaaaa tatatcagct aatgttgata gagataataa tgtcattaga gttaactgga
     9421 gtagtatatc tcataaggac tgtacttaca gtatatatgt aaaaaataat actaatggag
     9481 atggagaatt tactctatat caaagtgata tacagggaaa tgagcatact ataagtattc
     9541 ctgaaggaga tttaaatcta gatatttatg ttgttgcaaa ttataatgga aatagctctc
     9601 aaaatagtga tgttataaat gtaagataga tttgtataca aattgaaagt gttgagttta
     9661 tttaaatatt aaattaagaa aaaggatagt atagggtaat tatactatcc ttttttgtta
     9721 caatatatag gtttaaaatc tctagtaata taaattataa aaagtcttat ttacacaaat
     9781 ctatagatat tttttttatt aatcaatatt gtcttaaatt gaacttagtg cggcttctat
     9841 tgcatgtatt actgtcgtat caaatagtgg aatagaagtg tcattttgtt taattaaaag
     9901 acctatttct gtacatccta atataacagc ctctgcacct tgtttgccaa gcttatctat
     9961 aatagataag aaagcttttt ttgaagattc agatataatt cctaagcaaa gttcattaaa
    10021 tattgtgtca tttactatta tacggtcttc ttcatttgga attaagactt caattccatt
    10081 attaataatt acatttttat aaaaatcttg ttccattgta tatttagtac ctaataaggc
    10141 cacttttttt atttcttttt cccttaatac cgtagcagtt acatctgcta tgtgaagtaa
    10201 tggaatatgt atactttctt gtattttatc tgatacctta tgcatagtat tagtacagat
    10261 aacaataaaa tctgcccctg cctcttgtaa tttaattgca gcatcagcta aaattttggc
    10321 gcttttttcc caatttccac tagattggca ttcttcaatt tcttgaaaat ctacactgta
    10381 aagtatacat ttagaagagt gtagtccacc taatctttct tttattactg tattaataac
    10441 ttgataatat gtaatggtac tttcccaact cataccacca attaagccta tagtcttcat
    10501 attatacctc tcttatttat ttattttgaa atagctattt acaaatacaa gtatagcatc
    10561 taaaatagaa gcatacaatg ataaaattgt atggagtaca attataatta agaggagtaa
    10621 ttatatgcca ataaattctt ttgaaaacta tcctatgaca tggaaaccaa aattagataa
    10681 ccgaaaacca ccaatatata aaactttagc aatgttatta gaagaagata ttaaaagagg
    10741 aaatttaaat cctggagata aactgcctcc acaacgggaa ttagcagatt ttctagattt
    10801 aaacttaagt acaataacac gagcatttaa actttgcgaa gaaaagggac taatatgtgc
    10861 aaaagtggga aagggaacat tcatttcttc tgatgtaaat gtaagtaata cattattata
    10921 tcaaacggaa tcaaaagaca taattgaatt agggacagtt catcctccgt atgagcaaaa
    10981 tacatatatt attgacttta ttaaaaatgt actaaaacaa ccagaaatgg ataggttttt
    11041 acaatacatg tctccatctg gcacatatat gcaaaaaaag tcaattgcaa aatggataga
    11101 aagaaataat gtatacacca gtgaagaaaa tatattatta tctacaggag ggcaaaatgc
    11161 tatatgtgca actttactag gattatttaa agctggtgat agaattgcta cagattcttt
    11221 aagtttttct ggaattaagt caatagctaa aatgataggt attcaattag tcccaattcc
    11281 tcaagaaaat aatgaaatat cgattgaata tcttgaaagt tattgtaaaa atgaaaatat
    11341 aaaaggcata tatcttatac cagattatca taatccaacg acacatacta tgtctgattt
    11401 atctcgtaaa aagatagcca agatagcaaa gcaatataat ataataatta ttgaagatgc
    11461 tataaatagc atttttagag atggtataca aactcccata ttttctttag ctagtgataa
    11521 tactatatat atttttagta catctaaatt tttatgtgct ggtcttagag tagcatttgt
    11581 agttgctcca aaacgatata tagaaaattt agaaaatgca ctatataata tgaatctaat
    11641 ggtttctcca tttacagcag aaatagtaca ccgtttatta tattctccta ttatagataa
    11701 aattatagaa gagaaaagag atgcaattat agaaagaaat gaagttgctg ataaagtttt
    11761 gtcagattat aatttgattg gtgataagaa ttgcagtttt cgttggttac ttcttcctga
    11821 tgaattggat ggaaaatcat ttgaaatttc tgcaaaaaat ctaggagttc aagtatattg
    11881 tgctgaacga ttttcagtag gaaactctac agttccaaaa gcagttcgta tttgtgtaac
    11941 agctccaaaa gatgtagaag aattagaaaa agggcttaat ataataaagt cacttcttaa
    12001 gagttaaatt tattttacta ttatttaatt aaataaataa aaaggcggag aaatttatta
    12061 taataaacct ccgcttttat tatttaatga aatactttac tttttattaa aaaatttaat
    12121 tttaataatc ttgaaaatat aatcgtttta tggtaaaata attcttaaaa atagaaaaaa
    12181 ttaatcattt gttaaaaaaa atgcataatt tattgtaatt tattgacgaa ggttgaaaaa
    12241 tattcataaa tgtgcgaaat aacagaaaaa atgcaaaaat taattcaatt tcatttgtta
    12301 taaaatctat atggcaaaat taaatctgcc tatcagaatg atataaacac accagaaagg
    12361 aggacgctat caaataatac aagtagttaa ctttgatagc aaaaaattat gaatgaacaa
    12421 actagaatat ccttagagag agctgctgaa ttaaaatcaa aaattgatga ttatattcag
    12481 gctagaaaaa cgattaacag aggtcttgaa aaagaagaag agataaataa acgaaaacag
    12541 aaaatattaa gtatcttaaa tggaactgaa gaggattgga ataactacaa atggcaatta
    12601 tcaaatagaa taacagatgt agatacttta tcaaaaatta taactctaac taaaaaagaa
    12661 aaagaatata taaaagaggt tggtactcaa tttagatggg caatatctcc atattatttg
    12721 agtcttatag acccagaaga tacatgtgac ccaataaaat tactgtctat accaacacat
    12781 atagagttgg aagatgaaca agaagatttg gacccaatgg gagaagagta tacaaaccca
    12841 gcaggatgta taactagaag atacccggat agattaataa taaatgtaac aaatgagtgt
    12901 gctatgtatt gtagacactg tcagagaaga agaaatattg gacaacaaga ttctcataag
    12961 tcaaaagcta ttatccaaga atctatagac tatatcagag aaaatgaaga aataagagat
    13021 gtactagtaa ctggtggaga tgctcttaca ttaaaagatg attatttaga gtggattctt
    13081 agccaactta aagagatacc acatgttgat tatgttagat taggtactag gactcttgtt
    13141 acaatgccac aaagaatcac agatgaattt tgcaatatgc taaaaaaata tcaccctgta
    13201 tatataaata ctcattttaa tcatccaatg gaaataacta aggaatctaa agaagcttgt
    13261 gaaaagttag caaatgcagg agttccatta ggaaatcagg cagtattatt aaatggaata
    13321 aataatgata aatttgtaat gagatgttta aatcaagaat tactgaaaat aagagtaaaa
    13381 ccttattata tattccaaag taaacatgta aagggaacaa aacatttcaa tacatcagta
    13441 gatgatggtc ttgaaatcat ggagtattta agaggatata catcaggaat ggctatacca
    13501 acatatatag taaatgctcc aaaaggaggc ggaaagactc ctttgcttcc tcaatacctt
    13561 gtatcaaaag gaacagatta cgttatgctt agaacatggg aaggaaaagt tataaaaatg
    13621 gaagatgaac ctgctgtaga tataaagaaa cttataaaag aacaagcgca ggattaaaaa
    13681 agtatttaaa gtttataaaa agaaacaact ttaaaataaa aagaaaatgg atgtcaaatt
    13741 tatatcaatt tgcatccatt ttttattagt tatttattat tttgttgttg attaactaat
    13801 tcaagatatc cacttgggtg gaagtaatat atctcaaaag tattttcatt tacaaagtaa
    13861 ccaatatcac catctgcatc tggtaaaaat acataaccat taagattttt agcttttaat
    13921 gcttcaaaat cactagcatt tccttgatat atgtaatcaa cttcattgtt gtattttact
    13981 aaaagttctt tagcttgttc ttgagtcaat ttagtatttt ggcttacagg aacattcttt
    14041 tgagaatctg cataagaaga ttcatttact ggtgcagtta ataacaaaca agataaaact
    14101 aataaagata taagtgcttt tttcatacta aaaccctctt tcatgtttaa ttatataaga
    14161 ttcattactt aataatattg tatgtttcct ataattaaat attaccatta aagagaataa
    14221 tattacagtt acacagctgt aacttttgat tacaaaatcg taattaaatt gtatttttat
    14281 tccatttgta ttttatcatg tctacgattt acaaactcac agaagtatat ctttataacg
    14341 cccatcacag gagcagatag aatcatacca ggtataccaa agaatcctcc accaacagta
    14401 actgctagga ttgtaaagaa tgggctaaga cctagttgtc cacctacgat ttttggctct
    14461 ataaatgtaa cttcaacttg ttgaattatt attaagtaaa ttaaagaagt taaagcaatt
    14521 gttggattgg agaataaatt aattattaca actggtgcca tacctattac tggaccaaaa
    14581 tatggaacca tattcatgac acccataagt gtaccaaata atagtgcata ttcagatttt
    14641 aaaaagtaaa gaccaattcc agaaagtact ccaactataa aagaatctaa aatcttacct
    14701 gtaaagtatt taccaatatt taaatttaag ctttgacata gttctattat aaagtctcca
    14761 tattttttac ctaaggaaat ataaactact ttttttgtaa acagtaagaa tttttctttt
    14821 tctaataata tatagaaaca aattataaaa cctaaaagaa attgtactat aaattttcca
    14881 actgaaaatg tagtgctaaa tatcccacca agtgaaccaa taagcaagtt actaaattta
    14941 ggcattacac tcataatttt gtcaccatac tcttttaatg tattagggtc aactgattgt
    15001 aaactcttgc ctaagtcgaa taaaaactgc tctgttttat ctatgtatgt aggaatttga
    15061 gcaactaaat cagctaaact atttactatg gatggaactg taaataatat aaacgaaatt
    15121 agtataacta tgagtacacc ataagtaaga agtaacgaat atattctctt aaaatttaat
    15181 ttactttcta agaactttac tagtgggttg aatatatatg ctaatacaaa tgctattata
    15241 aatggtgtta gtagtgacat taataaactt atcactccaa aaaaatattt gtaattatca
    15301 ataactttaa tcaagatata tgaaacaact acagtgagta caatatttag ataaattttt
    15361 ttatcttgca aaataacaca acctttctta tatttattaa atttagatat acttaaatct
    15421 aagttaaagt taatatttat agcttattat acaataaaaa gcagtattaa tatagtatga
    15481 ttaatatgaa attataaata cgttaaaact ttatgtttat agctaaaata ataaaaatta
    15541 tttatattgt gtttatatat aatctaaaat gaaaaaaagt ggtgcctttg attaaactaa
    15601 gtttttataa tttaaatttt gta
//
LOCUS       DAMJYC010000093        15449 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJYC010000093 DAMJYC010000000
VERSION     DAMJYC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.096"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            451..1857
                     /locus_tag="PC472_003086"
     CDS             451..1857
                     /locus_tag="PC472_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2728395.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1885..2733
                     /locus_tag="PC472_003087"
     CDS             1885..2733
                     /locus_tag="PC472_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2728396.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLSEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3394..4086
                     /locus_tag="PC472_003088"
     CDS             3394..4086
                     /locus_tag="PC472_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728397.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4083..5018
                     /locus_tag="PC472_003089"
     CDS             4083..5018
                     /locus_tag="PC472_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728398.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYMDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            5166..6089
                     /locus_tag="PC472_003090"
     CDS             5166..6089
                     /locus_tag="PC472_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728399.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6073..6843
                     /locus_tag="PC472_003091"
     CDS             6073..6843
                     /locus_tag="PC472_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728400.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6853..7635
                     /locus_tag="PC472_003092"
     CDS             6853..7635
                     /locus_tag="PC472_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728401.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            8012..8548
                     /locus_tag="PC472_003093"
     CDS             8012..8548
                     /locus_tag="PC472_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2728402.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8580..8969
                     /locus_tag="PC472_003094"
     CDS             8580..8969
                     /locus_tag="PC472_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2728403.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            9006..9392
                     /locus_tag="PC472_003095"
     CDS             9006..9392
                     /locus_tag="PC472_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2728404.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9519..10961
                     /locus_tag="PC472_003096"
     CDS             9519..10961
                     /locus_tag="PC472_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2728405.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            11258..12073
                     /locus_tag="PC472_003097"
     CDS             11258..12073
                     /locus_tag="PC472_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2728406.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            12675..13859
                     /locus_tag="PC472_003098"
     CDS             12675..13859
                     /locus_tag="PC472_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728407.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(14236..15345)
                     /locus_tag="PC472_003099"
     CDS             complement(14236..15345)
                     /locus_tag="PC472_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2728408.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         5512 a         1934 c         2783 g         5220 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagtt
       61 tgggcaagta gtttcttgta aatctagtta tgtttcaacc aatgataata taatttcatt
      121 agtattagct ggtaattaag ttaatttgac tagagataag taaattgatt taaaaggaaa
      181 taagtaaatc aacaattaaa tacatctaaa agtaagttta ttgcaatgaa ttgttaaaga
      241 aaataattaa tatggagtaa agctattgca atgaagtaac aaagaaaata attaatatag
      301 agtaagttta ttgtaatgaa ttgttaaaga aaataattaa tatagggtaa atctatctca
      361 atgaaataac aaagaaaaga gttaatatag agtgaactta ttgcaatgaa tcaacaaaga
      421 aaataatcaa tataaaaagg agaaatattt atgaatacag gatttccaaa agacttttta
      481 tggggagcat catcttctgc atttcaagtg gaaggagctt gggataaaga caataaagga
      541 aaaaccgtag ccgactataa ttcttttaaa aagtctcatt tacaggcaga tactaaagtc
      601 gcatcagact tttaccataa ttatgaagaa gatattgaat taatgaaaga attagggatg
      661 aaaacatatc gtttttcaat ttcatgggca agaataatac cagatggtga aggagagata
      721 aaccagaaag gtttagaatt ttataataag ataatagaca aactgataga gtgtgatatt
      781 gagccattcg taactttata ccattttgac cttccattta aattagtaga gaaatataat
      841 ggttgggaaa gtagagaaac tgtctatgct tttgaaagat ttgctaaaat atgctttaag
      901 cattttggag atagggtaaa gtattggcaa cctcataatg aacagaattt aattgttaga
      961 gtggaggaga gaattaatat atatgatgaa acagattcat ggaagataga taaaatgcgt
     1021 gctcagatgg attataatct ttgtttagca catgcattag cagtcaatgc atgtcatgag
     1081 atgataaaag aaagtaaaat tggagcagca gtatcatcat ctgtaacata tcctcttact
     1141 tctaagccag aagatgtata tgcagctaga atgaatgata attttaaagt gtactatatg
     1201 cttgatatgc atcattatgg agaatatcca gggtattata tgaagtatct tgaaaaaaga
     1261 aatatagttc ctcacatgga agatggggat aaagagatac ttaaaaaagc taaaatggat
     1321 tttatagctg taaattatta taggacaaat tgtgcagaag ctttacctga agattcacaa
     1381 catccatttg gattaagaga aggaactgtt gattttagta tgtatggact atttaaaatg
     1441 tcaatgaatc ctaacttaga ggcttctgag tatggagcag caatagaccc atcaggtctt
     1501 agagttgcct taaatgaata ttggcaaaga tatcacttac ctgttataat aacagaaaat
     1561 ggtctaggag ctaaagatat attagaagat ggaaaaattc atgatgacta tcgtatagac
     1621 tatttaagaa gtcatataaa tgcatgtaaa ctagctattg aagatggtgt agagatgatt
     1681 ggatattgtc catggtcatt tacagacctt ttaagttctt ctcaaggttt taataagcgt
     1741 tatggacttg tttatataaa tagaactgac catgaagttt tagatttaaa aagaatcaag
     1801 aaagatagtt tttactggta taaagaagtt atagagaaca atggaatagt aaaataaaca
     1861 taaatatata ttgagggttt tagtatgtac attacaaagg tattaaataa ttcgttgcta
     1921 cttgcaaagg atgataatga tgaagaaata atattgatgg ggaaaggcat tggccataat
     1981 tataaggccg ggtctgagtt aaataaaaaa gatatagaaa agatttttgt tcttcatgat
     2041 gaaaatataa aaaagagtat tatacaattg gcaacagaaa tagatgcaga atattttggt
     2101 atagctcaaa tgataattgc atatggaata gaaaaatata acttaaaact tatgaaccat
     2161 atatatttgg ccttaactga ccacattgcc tttgcagtga gacgttttaa agaaggtata
     2221 atgattgaaa atcactattt gttcgaaatc aaggaattta atccaaaaga gtatgatata
     2281 ggaaaatatg ctattactgt ttttaaggag gttcttggtt tagacctatc agaagaagag
     2341 attggtaata tagcaggcca ttttataaat gcacaacaag ataatcctta tagtgataga
     2401 aataagagaa gtgctaaaat tgtaaatgct attttgcaaa tagttcatta tcacttttct
     2461 attgtatata ataaagagtc attttattat aggagatttg taatgcatct aaaggccttt
     2521 tcacaaagat ttctggctaa tgaaccatca agagaaaaaa tagattttat atatgagcaa
     2581 gttcaaaaaa attgtaagga agagtatgaa tgtgtgaaaa aggtaggagc ttatatgtat
     2641 aaggaatact ccagagaatt accaagacaa gaggagttat atttgatgat tcatattcat
     2701 aagattttag gagaattatg cgaaaatagt tagaatatct taatatgcta gtaatattat
     2761 aaaaaattaa aaattaacta cactttaaat atgaataatt gcattactta agggggttgt
     2821 cttaaaattg atttttttaa ttaaatttga agctcaactc cttttttaaa cctttatatc
     2881 attacaactt taatgtttat acgcctgtac tttagatgta tatctacttt ttacatttct
     2941 caacatgcaa acccaccatt ttttgcagta tgttaattga aataaaccat tgagttaacc
     3001 ctaaatttat tctggactta taggaatagt tccaaagata ctttaaaata ttttctggaa
     3061 taattgctta tttcacatat gttttattgt gatgttttga tttaataaat tcttcaactc
     3121 tgtatatagt aaatgtatta tcatgtttgt tttattcaag cacacttata atatgcttga
     3181 ttttttcaca atgatgaaat atactaatgg gtgttatagg tataagaata gcattacact
     3241 tgaagaagta agtgataaat gagatataaa tcctcatatg ataaactact atttcattga
     3301 ggagcatatt ctaagtgatg tcaagatact acaatatgac taaattccta gtgataaaaa
     3361 agtctacgaa tgagtgtaga aaggggaact aagatgaata aagttttaat tatagatgat
     3421 gataaagatc tttgtatttt gttaaaaaag agtatctcaa ttgaacagat tgaagcaaac
     3481 tattgccatt ctgggagaga tggactggtt gaactttcaa aacaagatta tcagttagta
     3541 gttctagacg tgatgatgcc aggtatggat ggttttgaaa ctatggaaca aatacgaaaa
     3601 tatagcagta ttcccatctt aatgctgaca tcaaaaagcg ataattacag caaagtgcat
     3661 ggattgcgag ctggtgcaga tgactatcta accaaaccat ttgaaattac agaatttact
     3721 gctcgtgtac tgtctttaat ccgacgttac acccgtttta atccaaaaaa tgaggagcca
     3781 cagcaacttt ctttcaaagg tttgttgatt gaccttgacc agcatacagt tactaccaat
     3841 ggcgaaccta tgttgcttcc acccaaggaa tttggtgtac ttctttactg tgcaaggcat
     3901 cagggtaaga ttttgacaaa acagcagatt tatgaagccg tgtggggtga gccatatgta
     3961 tatgatgaca gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat tgaactagac
     4021 ccatcatctc ccatctattt gcaaacaata aaaggaattg ggtatcaatt caacacggag
     4081 gtatgaaaat ggacgaacaa acgataattc ttttcttact cttgctgtcg attggtctac
     4141 tttttctttc catatttgct ttttccaagc ttgggcatat ttacaagcgc ctaaaagata
     4201 tagaggagat actggctgat gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc
     4261 catgtgatgt tatggctcca ttggtttatc agcttaatga gattgtctac gattatgaaa
     4321 ataagttact ttctctgaaa aagtcggata aagctagcaa acaacttatg acaagcttat
     4381 ctcatgatgt tcgtacacca ttaacaactc tgattggtta tatggatgct gtacacagtg
     4441 gtatagttat cggacaagag cgggaggaat accttgaaat cgctaggcgt agagcctacg
     4501 acctcaagga ttacattgat gttctttttg actggtttcg gttgaactct gatgaattta
     4561 cgctgtcaat agaatctgtg gaaattgcag agttgtcacg caatattttg aaagactgga
     4621 ttcctatatt ccatgaaaag aaactgaatt ttgaaattga tattccagaa aatcgactaa
     4681 tggtaaacct cgacccagat ggatattccc gtgtggtcaa taatctggtt caaaatgttc
     4741 ttgctcacag taaggctcgg caaatcaaga ttaccatgag tgaggattcc agaatggtat
     4801 tactccgtgt tgaggatgat ggtgttggga ttgccagaga aaatttacca catatttttg
     4861 agcgactgta taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta
     4921 ttgtcagtca aatggtggag cgaatgggtg gtcaggtttc cgtagaaagt gagattggaa
     4981 agtataccgt atttacagtg agcttaccat taatataaaa ttgtttttcc cccatgtgac
     5041 atgtatacac agggggattt tttgtaagtt ataaagttgc aaggttaata caaggttgtt
     5101 gcaaggttaa tgtaagattg aggtggtatg ataactttaa taaacaaagg aggcttttat
     5161 tatttatgaa taatttaatt attgaaacaa aaaatctcac taagcaatat ggtgctcaaa
     5221 agagcgtttc taatttgaat attcacgttc agaaaggtcg catttacgga ttgctaggaa
     5281 gaaacggtgc gggtaagacg acaactatga aaatgttatt gggtctaaca aaaccaacta
     5341 ctggagaaat taaaatattt ggtcaggaca ttcacaataa cgagaagaag cttcttcctc
     5401 gaattggatg tttgattgaa tcaccagggt tttatcctaa tctgacagga acagaaaatc
     5461 tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg
     5521 atgtagtcgg tttgccaaac aatgacaaaa agttattttc tcaatactct cttggtatga
     5581 aacagaggtt ggcgattgct cttgccatta tgcatgaccc acaactgttg attttggacg
     5641 agcctatcaa tggacttgat cctattggta ttgcagaagt aagaacgttt attcgtgatc
     5701 tctgcacaga acgaggtaaa actattttga tttccagtca cattctttct gaaattgcct
     5761 tgttggcaga tgacattggc attattgacc atggtatttt gttggaggaa gaaagtcttg
     5821 cccaattaga agaaaaaaac agtaaataca tacattttgc tgtatctgat gcagcacagg
     5881 cagcaaggct tctggaaacc caacttaacc tgaaaaattt caaagtagca gataattaca
     5941 atattatgct atatgataca gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa
     6001 acaacattga ggtatcagaa tctcatttat gcgaagatac tcttgaagat tacttcaaga
     6061 aagtaacagg gggtgaagga attgcttgat ataatttcat gtgaattttc aaaactcaag
     6121 cggttaaaat ttatactgat ttctatttta ggtgcttgct tatttccaat tcccgcaaca
     6181 attttgattg caaaggacaa cttgcccttt gagcaattgt tcaaattagt tgtcaatttt
     6241 ggatattttt tgctattacc aattgtatta agtattgttg ctagccaact attttttata
     6301 gaaagagata atgatgtatt aaaaaatctt gcaacagtgc cagtgcaaaa aggaaaactg
     6361 gctctggcaa aattggctgt gttgctgttt atatcattgt tctactctgt tgcagggtta
     6421 ggagctacta tcattggagg tttcattgtt ggaatagtag agggggttgc tgtaaaatta
     6481 ggaatgagca ttgcttttgg aattatgttt tttgttactg tacttcctgt tgtggtactc
     6541 attgtatatt tcaatcgcag ctatattttt tctattatac tttcttttgt atatgctatt
     6601 ttcaatttta gtatatcctt aaatattatc aattttgaac caaataaccc aatattaagt
     6661 gtattacctg ctcctgttat aatgcgttgg tggatggcat tttggggaga tcccactggc
     6721 gaatacacgg cacttagaca gccatatttg ctctcaactc ctgcttgtgt aggtatttta
     6781 tgtttgataa ccattatagc agtcctgctg atttgtatga tatacaaaaa gcaggagaat
     6841 taggaggtat ctatgcttga tataattact acagaatttc aaaaaataaa aagatataat
     6901 attctatgga ttggtgttgt tgctgttctg ttttcagcac tattggcagt ttttcaggtt
     6961 gtaagttcac acggcagtga ccctctaacg tatgagagct ttgcaaacgg agttatctgg
     7021 aataatttca gtcttagttt tccatttggg attacactta ttggaggata tcttattaac
     7081 cgtgaatata cagaccaaac cttaaaaaac atcctaactg ttcctatttc attacgcaaa
     7141 ttgctagtgg gaaaattaat tgcggttgga ggaattactg tattgttaag tcttttcagc
     7201 ttcttatgca catttatttt ggggttgttg ttttgcaata tcaatatatc tgttacactc
     7261 gttgtaaaat ccttagttca gattttgtct gttaatctgt attgttatgt tgctgttttg
     7321 ccgattattg cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt agggattgca
     7381 tttgtatacg gtttttgtgg tgtgtttgtt gctggaagaa atctgacaga cttttatcca
     7441 atcacagcag gtcttggtct agttggatac aacaatggtg ctggtactgt ttatcagcca
     7501 cttatcggtg ctatgacttt gattgtaatt ctaatcttga caactattct acttgtattt
     7561 acacctaatt atgataaagt tatgaccata tctaagaaaa aagggataaa gcataaaaag
     7621 tattctcata aataacataa atattgtttc tattatttaa gttgctagac agtgaaaaga
     7681 aaaaatctgt cgtacagtgt ctatatgctc atgtattcaa gaagctacag ttttgatttt
     7741 tcataactgt agcttctttt tgtatttatt aaatcacgac tatcataata cttgcaatag
     7801 gtatagcgac aagagagagt actgtcatat gtgttttata tattacatta agtatattct
     7861 aaaatatatg ttttaaataa tatatttgaa aataggaata tttaaataaa aaaaataata
     7921 ttatatatag ttgacaaaaa ttggcaaatg agatactata taaatataga aatagtaatc
     7981 attactatta ttaaaaaggg aggaattaat tatgaactta aaaggaacta aaacagaaaa
     8041 gaatttaatg gcaggatttg caggagagtc agaagcaaga aataaatata catactacgc
     8101 ttcaaaagca agaaaagaag gttataatca aatagctgct atatttgaag aaactgctaa
     8161 caatgaaaag gaacatgcta aattatggtt taaactttta catgatggta tgccttcaac
     8221 agaggaaaat ttaaaggatg cagcagcagg tgaaaactat gaatggactg atatgtatgc
     8281 aaaatttgct aaagaagcta gagaagaagg atttgacaag atagcttatt tatttgaagc
     8341 tgttggaaaa atagagaaag agcatgaaga aagatactta aagcttttag aaaacttaaa
     8401 tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg caatgtcaaa actgtggtca
     8461 tgtgtatgta ggtactgaag caccagaaaa atgcccagta tgtgaccatc caaaagcgta
     8521 tttcaatata aaagctgaaa attattaaaa attatcatta ttaaaaagga atgttaacta
     8581 tgaaattttc taaacaacga gaactgattt taaatgaaat attaaataat ccggttcatc
     8641 ctactgcgga ttacctatat gaaaacttaa aaaaagataa tccaaattta agcttaggaa
     8701 ctgtgtatag aaatttagct cagctaacgg aacatggctt tataagaaag gttagtattc
     8761 cagggtatcc agatagattt gatggtcgaa tagataatca ctatcatatc atatgtgaag
     8821 tatgtggaga ggtatacgat ttagaatccg aagttcttaa taatttacaa gaattaatat
     8881 ctgaggagac agacataaaa ataacatctt ataacataag ttttaaaggg atttgtaata
     8941 attgtaaaag gtgtagccaa gtaggttagt tttcaaattc taaaaataat taatgaggtg
     9001 agtttatgtg tagtgaacaa aaatttttta tatgtgagac ttgtggaaac cttgtaggaa
     9061 tgatacaaag tggtggtgta cctattttct gttgtggaaa acctatgaag gaacttgttc
     9121 ctaatacaac agatgcagca gttgaaaaac atgtacctgt aattgaagtg gatggaaata
     9181 atgttactgt taaagtaagt agcacaactc atccaatgac taaagaacat catatagcat
     9241 gggtgtatct tatgactgaa caaggtggtc aacgcaaatg tctagcagta gatggagaac
     9301 ctgtagttaa atttgctttg aatgatgatg ataaagtgat ttctgcctat gcttactgta
     9361 atttacatgg attatggaag gctgaactgt aatatttaaa taaattgagt caaaaaggag
     9421 gctataaatg tattttcata caaatagcct cctataaatt tatataaatt caagttttaa
     9481 ttttgtttaa tgtcaatata atataaggag aaaataatat gtcaatttat aaatgtagtg
     9541 tttgtggata tatttatgat gaatctaaga atgataaaac atgggatgaa ttaagtgaag
     9601 attgggaatg ccctgtatgt acaaaaggtc gtagttattt tggaaagata agtactgttt
     9661 actatgaaga agatgaaaag atagcagaag acatagttga agatgaatct aaattaaata
     9721 ctgaaaaaga gggagattta aattacttaa gtacatattt aagaagagat gatgaagttg
     9781 agaaacatat ggatataatt cacgaaatgg ctgttacagg aaaatctata atagaaccta
     9841 tgagaactaa actaccagta atttcttggg atgatatttt aattatgggt gctcaattaa
     9901 atccattacc tttaaatgag catgatgaag taaatacaac aacaataata ggaaaaaaag
     9961 caaagaaacc tatgattata gaaaatccag tttatatttc ccacatgtca tttggagctt
    10021 tgtctaaaga actaaaaata gctttagcaa agggtgcagc ccaaaataag acagctatgt
    10081 gtagtggtga aggaggtata ttaccagaag agaaggaagc ttcatacaag tatatttttg
    10141 agtatgtacc aaataaatat agtgtaactg aagaaaatct taaaaattct gatgctatag
    10201 agataaaaat tggtcaagga acaaaacctg gtatgggagg tcatcttcct ggcgaaaaag
    10261 taactgaaga aatagcaaaa gtaagaaata agcctgtagg tcaagatgta ataagtccat
    10321 cttgttttga ggaaatacaa tccaaagaag atttaaagaa acttgtagat gaacttagag
    10381 aagtttcaga agggcgtcca ataggtgtta aaatttcagc aggtcatatt gaaaaagata
    10441 tggaatttat agcttatgct aaacctgatt ttgtaactat tgatggtcgt ggtggagcta
    10501 ctggagcaag tccaaagttg cttaaagatg caacttcaat tccaacaata tttgctcttt
    10561 atcgtgctag aaaatacata gatacacatg gattagatat agatttggtt ataacaggtg
    10621 ggcttcgtat atctactgat tttgctaagg caattgctat gggtgctgat gcagttgcta
    10681 ttgcaagttc tgctcttatg gcagcggcgt gtcaacaata tcgtatatgt ggttctggta
    10741 agtgtcctgt aggagtagct actcaggatg aggaattgag aaaaagatta catatagaaa
    10801 acagtgctaa tagagtggca aactttttaa atgtaagttt agaagaactt aagacttttg
    10861 ctagaatttc tggtcataaa gatatacatg atttaagtgt tgatgattta tatactgtta
    10921 actctgaaat atcaaattat acaaacatac agcatgtata gattgaagtt tgaaactatt
    10981 agattttaga tagctataaa agtaaatgat aaatatattt tataaaagag agtgtcttaa
    11041 aatgaacttt ttagttcata agtcactctt ttttttatga agccaaatat atttatcatt
    11101 ttagcaatga aaaagaatct tatctcttta ttttgagata gactccttct atttatgaaa
    11161 attaaagata ttaaattgta aaatattctg tgatgtcgta ctattaaact aaagattctg
    11221 tactttaaag taaataaaaa tttaaggagt gattattatg gaaagattga agatacaagt
    11281 tttagttgac aataatactt atattgatag atactttgtt ggagaaccag cagtaagcta
    11341 ttatattgag attgatggaa ataggatttt gtttgactca gggtattcag atgtattcat
    11401 atcaaacgct gaaaaattaa atatagattt aggaaattta actcatgttg ttttttctca
    11461 tgggcataat gaccatacaa gaggtatcca gtttctagaa aataggtatg atttatctac
    11521 agttgaactt atttcacatc ctaactgttt tatacctaga aaacatggag agaaaagcat
    11581 aggagctcca ttttctgctg aggaaattaa aaatattttt atgtataatc ctaaggataa
    11641 gccatttaat ctaagtaaga attgtgtgtt tttaggtgaa ataccttcta taaatgattt
    11701 tgaaaaaaga gctaaaattg gtaaatgtaa aataggagat ttgtgggaag atgattatgt
    11761 tttagatgat tctgctatag tttgtaaaac tgataaaggt atttttattg ttacaggatg
    11821 ttctcatagt ggaatttgta atatagtaga gtatgctaaa aaggtatgtg gagatgatag
    11881 agttattggt atactaggtg gttttcatct ttttgaattg aataataggt tagatagtac
    11941 aatacaatat ttggataaag aagaaatgag aatgttgtac ccttgtcact gtgtttcttt
    12001 taaggtaaaa gcgaagatga atgaaatatt gaatataaat gaagttggag tagggctgac
    12061 cataagtata tagtatttga aagtatataa gatttaagtt gaaggttaaa aagatatttt
    12121 ctatagaaaa tatctttttt gttacgttta ttttaagaga aatcaatata cttttatatg
    12181 aaatattgta aaaaaattta tttttaatga gaatatattt tcattaataa acaattttat
    12241 tatgtattaa taatgttaga ataaatataa ataaatcaaa gttacttaca ttacagtata
    12301 ttttttcaca aaatatattg atttattctt ttaaagtttg ataattccaa tgaatgtaat
    12361 aaaagaagga attttatact atacgattat tattatataa taataaagaa aaaattatat
    12421 aataataaag aaaaaatgta taatatttaa cattaaaaaa gatatttcat aaaattaaaa
    12481 aacaaagtga atttgaatac tatttaaaaa ataaaataaa tatataaaaa atatttaata
    12541 taaaggtgga aataagttga aaatgtaaag tcattggaaa aatatagttg aatttaatga
    12601 gcaaaaatgt tattgtttta tcaataaagt gttaaaataa aaactttcaa tatcatttta
    12661 gggaggatga tttcatgcaa aaaagtgtaa agaaagcaaa agtaacgggc tctatgttag
    12721 ctatttttgg tgttgcaagc gttcttttta gttcacatgc aggaggagga tttgctacag
    12781 ggaatcaaga aactcagtac tatgtgcagt atggatggac agcaccactt atggcaattt
    12841 tagcaatgat tatacttact gctaccatga gagaagttat cataatgtat aataataaca
    12901 attgtaggaa ttataaggat ttattttgtg agctttggag accatatcca aaacttgaaa
    12961 taatatggga gatatattac tatctaatgg ttttaatagc tgtaagtgct gttatagcag
    13021 gtgcagcagc agtatttcaa agtataggag taaattattt tgtagctgta tttattattg
    13081 gagttgtact tcttgtcttt actatatttg gtgctatgct tgtatctaaa gcagctacag
    13141 caatgacaat agctattcta gtatgtactc ttactatttt tattgttggt ataaaagcta
    13201 aagtaccaga aataactgaa atattatcaa atagaacgag tttcacacct ggctatatca
    13261 agccaatact taatactttt atttatgcag gttttcaatc ggttgttata cctacacttg
    13321 caggatgttc aagaccactt cttaagaact caaaagaagc tactaaggca atgataattt
    13381 catttgttat gaatgcaatt gcattaggac ttgctgtaac tatgctaatg ggatggtata
    13441 gagaaattat tgctgctggg caaacaacac tccctactct ttatgttgca ggtcaatctg
    13501 gaaatcatac tatatacata atttataatg ttgcattatt cctttgttta atgtctacag
    13561 gagtaactac tatatttggt cttgtaaata gatttgaaga ccataaagca ctttctttcc
    13621 tttcttcaag aatgaaaaga agggtattta cagcttgtgc aataatggta gtgtcaatgc
    13681 ttatatcact tacaggatta agtaatattg ttaaatatgg atatggatat tgtggttatc
    13741 ttggtctatt cactatagta attccattcc ttacactagg acattataag aataagaagt
    13801 ttgcaaagga aaatccagaa gctaaatggc cagcggaatt aaatgaaaat gtaaattaga
    13861 tgtagtagca aaaaatattg ctaaagagaa tactatagat aaggatacta tagataatat
    13921 atctgcatgt ttattaagat tatttgcaaa atctaatatt aaacaactat aaacaagcaa
    13981 tataaaacta agaataaaag ataaaaagag ggtgtctcaa aatggacttt tagtttataa
    14041 ggcactcttt tttatatgaa gtcaaatata ttttcattat tttgacaata aaagagacta
    14101 atcctacttt ggatagcctc ttttttcttt aatataaaat agtataacag caaagtatag
    14161 attgatacat gttttctgta gctttctata tattacagtg tttgtgtggc attggtattt
    14221 taagcaatct ataaattata attctataat tcttctacta attttagatt ctatagccgt
    14281 ctctatttca cttaacattc gttcattttt agggtcaata aaagcacatg tatatccttc
    14341 ttcaccagct ctaccagttc taccaatacg gtggatgtag tcttctggtg tttctggtaa
    14401 atcgtaatta tatatatgac ttactccaga tatatcaagc ccacgagaag ctacatctgt
    14461 agctattaag tattgtatat ctaaatttct aaatgatttc attattcttt ctcgttttga
    14521 ttgagcaata tcactatgaa gtttttgaca attatatcct cttatagcaa gagcttcttc
    14581 aagattgtca acccttctct tagtcctaca aaatattatt gccataaaag gattatcttc
    14641 atctagtacc ttacataagg catctaattt ttttctatct gtagtttcta ctacttcttg
    14701 ttttatatta ttaagagtga tttcttcttt ttgaatagaa actacaacag gattttttgt
    14761 gtatctataa gctaattttt ttacttgaga atttatagtt gcagaaaagc atagtgtttg
    14821 ctttttgttt gaagtctctt ttaaaatagc ttccacttca tttttaaatc ccataagcaa
    14881 catttggtct gcttcatcta aaacaaaagt cttcaactta ctaaggtcta ttgtttttct
    14941 atttagatgg tctaaaagcc tacctggtgt agctataatc atatgaatat ttccttttag
    15001 tttctttatt tgagaaccaa tatcttttcc tccatatgct gcaagaatac ttacatcttt
    15061 agattcttta agtttcatag cttcttctgt tatttgaata gctagttctc ttgtaggaga
    15121 aagtataagt acttgtatat catttatatc taatgatata ttttcaaata tcggaagtaa
    15181 aaaagctagt gtttttcctg ttccagtttg agcttctgct attacatctt ttccttcttt
    15241 tattagttta atactttctt tttgtatagg tgtaggtgtt gttattcctg atttttttag
    15301 aatatttatt gtattttcac taatccctaa gcttttgaaa tccattgtta tcttaccttt
    15361 catgaaaaat tttctttttt attaaccaat taataatatc atatttgtaa gtatttttag
    15421 taaatttttt aattattatt tatatagct
//
LOCUS       DAMJYC010000094        15338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJYC010000094 DAMJYC010000000
VERSION     DAMJYC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.097"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(63..797)
                     /locus_tag="PC472_003100"
     CDS             complement(63..797)
                     /locus_tag="PC472_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2728409.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            922..1638
                     /locus_tag="PC472_003101"
     CDS             922..1638
                     /locus_tag="PC472_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2728410.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1772..2464
                     /locus_tag="PC472_003102"
     CDS             1772..2464
                     /locus_tag="PC472_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728411.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2454..3635
                     /locus_tag="PC472_003103"
     CDS             2454..3635
                     /locus_tag="PC472_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2728412.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3917..4372
                     /locus_tag="PC472_003104"
     CDS             3917..4372
                     /locus_tag="PC472_003104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2728413.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4362..5087
                     /locus_tag="PC472_003105"
     CDS             4362..5087
                     /locus_tag="PC472_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2728414.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5059..7440
                     /locus_tag="PC472_003106"
     CDS             5059..7440
                     /locus_tag="PC472_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2728415.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7645..8874)
                     /locus_tag="PC472_003107"
     CDS             complement(7645..8874)
                     /locus_tag="PC472_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2728416.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9474..10388
                     /locus_tag="PC472_003108"
     CDS             9474..10388
                     /locus_tag="PC472_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728417.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10412..11047
                     /locus_tag="PC472_003109"
     CDS             10412..11047
                     /locus_tag="PC472_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2728418.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAGGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11423..12139
                     /gene="pyrF"
                     /locus_tag="PC472_003110"
     CDS             11423..12139
                     /gene="pyrF"
                     /locus_tag="PC472_003110"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2728419.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVKEIENA"
     gene            12165..13208
                     /gene="carA"
                     /locus_tag="PC472_003111"
     CDS             12165..13208
                     /gene="carA"
                     /locus_tag="PC472_003111"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2728420.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13261..>15338
                     /gene="carB"
                     /locus_tag="PC472_003112"
     CDS             13261..>15338
                     /gene="carB"
                     /locus_tag="PC472_003112"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2728421.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSL"
BASE COUNT         6174 a         1693 c         2761 g         4710 t
ORIGIN      
        1 gattatataa agattattta caattaatac aatcttgctt ttttatttaa aaattttaat
       61 tattatttaa aattttttat cttctcttta tcccatataa acaatttctc agaattattt
      121 aaagcaccaa atacatttct tttaaaatca ggcatctctt tatttaaatt agcaataaga
      181 ttttttatgt actctctcct agtatgacca tttgcagggc agggatttgt gataatagga
      241 tagttagaat cttttgaaat tttcttagtc atatactctt ctatgtaaac cataggtctt
      301 ataactgtta aatcctgtct gtctaggtat gtctttgggg aaaaacaatt tactctaccc
      361 tcataaaaca tagacattaa aaatgtctca attgcatcat ctttatgatg accaagagct
      421 actttattac atccaagttt tttagcatta tcgttcaaag cacctcttct taaattagca
      481 cacaatgcac aaggattctt ttcttgtctt atatcaaata caatttcttt tatgtcagtt
      541 tgaaattcat aaaaaggaac atttatttct ttacacaatt tatctagtgg agaattatca
      601 acacctccag tatttaatgt tatggctatc aattcaaagt tttgtggaga aaatctttga
      661 taacttttaa gtatatgaag tagagttaaa ctatcttttc caccagaaag tccaaccgca
      721 attttatcat tttcttgtat catatcaaaa tcttgtatag ccttacgagc tttacttaga
      781 agtttttgta tagacatttt atcaccatcc tttaaaaata ttaaatttaa gatttattta
      841 agatttactt ataaaatact taagtcttat actctaaaat taacattata gcaaacataa
      901 caaaaaggag aagatttaat tatgaaaaaa tttaaattaa taggagccat tattttagta
      961 gtatttataa gtgtatttat atctaaagat agattactta agaaagataa agatccaata
     1021 gtagctgctc aacatgattc agaacaagat gttgaggcaa attctcaaaa taaaaatcac
     1081 tcaaatagta tatcaaatat attattggta aacaagacaa atggaattag caaaaactat
     1141 actcctgaaa atattactaa ggtaaacata ccttttgttg aagaagcaac agaggaagaa
     1201 aaacaaatgg caggagaacc tgcaaaggca gttgaagatt tagttaagca agctaattca
     1261 gaaggaatac agtttttagg gtcttcagca tacagatctt atgatactca actggatact
     1321 tatacaagaa gggtaaaatc tcaggggcga gaaaaggcag atgcgtatgt agcaaagcct
     1381 ggttacagtg agcaccaaac tggactttgt attgatttaa caaatccaga aagatggttt
     1441 gttggaagta caaaagaagc taaatggttg gcagaaaatg ctcacaagtt tggatttata
     1501 ataagatatc ctgaaggtaa agaagatata acaggtacag catatgaacc atggcatatt
     1561 agatatgttg gtaaggatgc tgccgaggaa atttattcaa aaggattaac tttagaagaa
     1621 tatttacaaa ataaataata taaatgtgta aataatttgt attatattgg aaatttagaa
     1681 attaataaat ttatagatta cactaactaa aatatagtat aattttatat gaataaagct
     1741 atatacaaag attaagttag gatgtgtaaa aatgaataat acagttcttg ttgtggatga
     1801 tgaaaaggaa ataagagacc ttatagagat atatctttta aatagtggat ataatgtaat
     1861 taaagctgaa gatggaaaag aggctctaga tattttaaaa aaggaaaata ttcatttaat
     1921 gatattggat ataatgatgc ctaaaatgga tgggatagaa gtttgtagaa aagttagaga
     1981 aagtttgaat ataccaatac ttatgttaag tgcaaaatca gaagacatgg ataaaataca
     2041 agggataatg actggtgcag atgattattt gacaaaacca tttaacccac ttgaacttac
     2101 tgtgagagtg aagtcgctga ttagaagagc atattatttt agtggtgcta ataatgaaga
     2161 taaagatgtc ataaaggttg gagtagtaat tatagataag gaaaaacata gtgtaacagt
     2221 ggctggaaat gaaataattt tgacatcaag agaatttgat atattatatt tactagcaaa
     2281 taataaaggc agagtatata gtacagaaga aatatttgaa aaggtatgga aagagaaata
     2341 ttaccaatct aataatactg taatggttca tatgagcaga ataagggata aaattgaaaa
     2401 atatacagat ggagaaaaaa taattcatac tgtatgggga gtaggatata aaattgaaaa
     2461 ataaaagttt attaaatcat atgtttcgac ccatattaga tcttataata agctgtgctt
     2521 taacttcaat gcttatttta acaagtatgt atataataaa atcattatat gtcaatagtg
     2581 atatgtctgt agttaatcaa cttgtatatc aagcaagaga tttcagaagt cttatagggt
     2641 atgatttatt ttatccattt ttatttacaa tcacatcatt agcaatttat tccatgatat
     2701 cgtacaagag aaataagcaa attgtagatt ttataagaaa gactaataga aaaaatcaaa
     2761 aattagaaat cattaaaagt gctgtaaatt taatggatga aggtaactta gaaaaaagta
     2821 tagatattga gtatgattta gatatattaa aggataaaga aaaggatgat attgatgaac
     2881 tagcacataa aatcaataat atagtccatc aacttagaaa tataactatt gaagagagac
     2941 aagctcagca gacaaaaaca gatttaataa caaatgtgtc acatgactta agaacacctt
     3001 tgacatccat aatgggttat ttaggagtga tagaagaagg taagtataaa gatgaagttc
     3061 agatgatgta ttatgttgat attgcatatg aaaaatcgaa aaatttaaat gttcttataa
     3121 atgatttgtt tgaattgact aaaatgcaaa ataatacaat aaaattaaac aaaatagaaa
     3181 taaatttagt agagttatta agtcaagtag tatctcaatt tgaaatttat tttaaacaag
     3241 aatttatggt tagtagaatt aattttaatg aagaaaaatt aattgtaaaa gctgatccaa
     3301 ataaattggt aagagcattt gaaaatctta taactaatgc tgtgaaatat ggaaaagatg
     3361 ggcattatgt agatatagtc acagagaaaa aggaagatat ggcggttgta aaagtgataa
     3421 attatgggga accaattcca gtgttagatt taccaaatat atttgataga ttttatagag
     3481 ttgaaaaatc gagaaataga aatgttggag gttctggatt gggtcttgcg attacaaaaa
     3541 atattgtaaa tttacatgat ggagaaatta ctgtttcaag cgataaatat caaactgtgt
     3601 ttgaagtaca actgccaata atacaatcag cttagttata aaataaaagt acgattatgt
     3661 gatataaata ttataacaat aaagtgttta caaaaataaa aaagtaatat ataatttaag
     3721 tcagtttaat aagatactta tcaagagtta ggggagagag ttagctcagt gatcctatac
     3781 caacctttta tcaggtggtc ctgctaacaa cgatgagatg acttgaaata atatactctt
     3841 cttatcaata atagatagga agagtttttt ttagaattaa tgtaacaaat tttaaagtat
     3901 aatggaggat taaataatgg aaaaagtaga aagttttaag ctagaccata caaaagtaaa
     3961 agctcctttt gtaagaaagt gtagtgtact tgatggagta aagggagata aagtaactaa
     4021 atttgacttg agatttttac agccaaatgt agaaagtttt ggaactgctg ctatgcacgg
     4081 tttagagcat cttttagcta cttatttaag agatacatta gatggtgtaa tagacttatc
     4141 tccaatggga tgtagaacag gattttattt aatactatgg ggagatgtag atgccaaaac
     4201 agttaagatt ggtttagaag aagcattaaa aaaagttctt gaatcagata aaatgccagc
     4261 agctacagct atagaatgtg gaaactacag agacttatct ttatttgggg caaaggaata
     4321 tgcaaaagat gttttagata aaggattttc tttaaatata tatggagaat aatttaattg
     4381 gaagagaaga tataagcata aatcaaagtg aagttttaag atatttacaa tatagaggtc
     4441 aagagataga taaagacctt aaatatacta ttgatgaatg tgtagctata actaaaaata
     4501 aaataaatcc tagatattta tctaggattt atcctattaa aataaataaa gataatgttg
     4561 tagaattaga gggaacaaat ttaacattag agagtaaaga tgtatatgaa cttttaaaag
     4621 aatgtaatga gtgtgtaatt atggcagcta ctatagggat agatatagaa aaagaaataa
     4681 gacgacactc ttattcaaat ttaacaaaag gactaataat tgattcatgt ggaacaacag
     4741 caatagaaga agtgtgtgac attgttcaaa ataatttaga aaaggttttt ttaaagcaag
     4801 ataaatttat aactatgcga tatagcccag gatatggaga tttaccaata gaaaaaaata
     4861 tagatatatt aaacgttttg aatgctcaaa aacaaatagg tctaacaatt acttctaatg
     4921 gaattatgat accgagaaag tcagtagtgg ctattatagg tatttctaat acaagaataa
     4981 ataaatctaa gaaagaaaag tcgtgtgaaa attgtaaaaa ctatagaaac tgtacatata
     5041 gaaaaggagc gaactcttgt ggaaataaga caatatctga aaaataatat attaatattt
     5101 gatggtgcaa tgggaacaat gcttcaacaa aaagggttaa agttaggtga aaatccagaa
     5161 gtatttggtc ttcaaaatcc agataaactt atagaaatac atacagcata cttagaggca
     5221 ggttcgaatg taattcttac aaatactttt ggatgtaatg aactaaaatt agatagtaag
     5281 tatactgtag aagaagtgat tgacaatgct gttttggtgg ctagaaaagc tatagaaaat
     5341 gtagataata ctaaaccaag atatgtagct ttagatattg gtcctatagg agaaatgcta
     5401 gagcctatgg gtactttaag ttttgataaa gcatatgaaa tatttaaaag acaagtacta
     5461 caaggcgtta aatctggagt tgatgtaata gtaatagaga ctatgatgga tttatatgaa
     5521 gctaaagtag ctgtattagc agcaaaagaa aactctgatt tacctatttt ctgtactatg
     5581 acatttgatg aaggtggaag aagttttact ggatgtatgc cagaatgtat ggtggctact
     5641 atagaaggtc taggtgtaga tgcaatagga gttaattgtt cattaggacc taaacaactt
     5701 ttaccaatag ttgaaaaaat agcatcaaga gctacagtcc ctgttatggt tcaagcaaat
     5761 gcaggtcttc caaatatagt tgatggagag gctatatatg atgttgatgc aaaagaattt
     5821 tttgaagggg ttaaaaagtt cgttgaagtt ggtgctacta taataggtgg atgttgtggt
     5881 acgaatccaa gttttataaa agaaatatct gagaatataa attctgttac aaagggatgt
     5941 atcgagaaaa ttgataaatg tgtagtctgt tcaccatcaa aatttgtgga agtacaatcg
     6001 cctacagttg ttggagaaag attgaatccc acaggtagaa aatctctaca agaagcatta
     6061 aaaaatgaaa atgttgatta tgctataaat ttaggtttag aacaagtaaa tgctggagca
     6121 caaatacttg gagtaaatgt tggtcttcca gagattgatg aaaaaaaact aatgccaaaa
     6181 ttaataagag agattcaagc agtagtagat acacctttac aagttgattc ttccaatgta
     6241 gaggccttag agcaaggact tagatattat aatggtagaa caatagtaaa ctctgtaaat
     6301 ggcaaagagg agtctttaga aagtatatta ccaatagtta aaaagtatgg ttcatgtgtg
     6361 gttggactta cacttgatga aaaggggata cctaaaaagg ctgaagaaag atttgaaata
     6421 gcaaaaagga tagttaatag agcagtttct tatgggataa aaccaaaaga tatatttata
     6481 gactgtttat cattaactgt ttcagcacaa caagaagaag caatagaaac tataaaagct
     6541 ataacaatgg taaagacact aggagtaaaa actatattag gtgtttcaaa tatttcattt
     6601 ggtcttccaa ataggaaagc acttaatgct tcttttctaa cattagcttt aggggcaggg
     6661 cttgatttag caattataaa tccaaatgaa tatagtatga tggaagctat caattctttt
     6721 aaaatattaa ataacacaga taaaggatgt ataaactata taaatcaata tagcaatata
     6781 aataattcaa aaaaatcgga ttcatctaca aagattgata aagatttgcc cttggatatt
     6841 ttagttgaaa gaggattaaa agatgaagca aaaaatgtaa cactgaatct tttaaaagag
     6901 aaggatgaaa attatatatt agatgagatt ttaattcctg ctcttgataa agtaggaaaa
     6961 agatatgata gtggagatat atttttacca caattgattc aatcagcaga aacggtaaaa
     7021 gtttcattaa atacaataaa ggaaacttta ttaagtaaaa gtagcaataa tgtatccaaa
     7081 ggaaaaataa tagttgctac agtaaaaggt gatatccatg atattgggaa aaatatagta
     7141 aaaataatgc tagaaaatta tggatatgaa gttatagatt taggtaaaga tgtccctatt
     7201 gaagaagtag tggaagttgc taaaaagaga aatataaaat tagtaggatt aagtgcactt
     7261 atgactacga ctgttcaaag tatgaaagat acaattcagg cactgagaga taatgagata
     7321 aatgcaaagg tatttgtagg aggtgcagtt ttgacagaag aatacgctga agaaatgggt
     7381 gctgattatt attcaaagga tgcaaagtct gctgttgaaa tagcaaagtt aaatttttag
     7441 catataaatt aaaataaagg ttttactaaa taggatattt tatatataat ataaatataa
     7501 gttttagata cttatgttta aaacaatagg aattaatcta taataatgag attaattaat
     7561 ataaaaaata tttatcatca acattaaaaa gggctgggaa gtctggtata acttccatca
     7621 gcccttttta gttaaaatca tatgttaaaa agcccaatta ccatttttaa aaatctgtat
     7681 ttgctttcca tcataagtct caccaataat atccatatca gcagaaccaa tcataaaatc
     7741 tacatgtgtt aaactgtcat ttacaccatg ttcatccaat tcttcatctt ttaaattatt
     7801 tccaccttcc agacaagtct tataagcaga accaattgca aagtggcaag aagcattttc
     7861 atcatataga gtattataaa aaattatatc cgtattggat attggtgagt tgtatggaac
     7921 aagagcaact tctcctaaat aatgagagcc ttcatccgtt tcaataagtt tttctagtgt
     7981 atctagtccc ttttcagcag agaaatctat tattttacca tctttaaatg ttaagaagaa
     8041 attgtcaatt atgtttccac cataaacaag aggttttgta ctataaacag ttccatttac
     8101 tttaaactta tgtggcattc caaaaatctc ttcagttgga atattcggat taaaacttat
     8161 tccattaggg tctttggatg ctccacttag ccaaacatga ccctttggca agtctagagt
     8221 caaatcagtt tttgaggatt tatattttaa agttttaaag ttatttttat ttagaaagtc
     8281 cattttagat ttaagatttt cattatgttc ttcccatgct tttatagggt cctgaccatc
     8341 tactcttgaa catttaaata tagcatccca cagtttttct attgctttat cttctgataa
     8401 atttggaaac actttttttg cccaatcttt tgttggcaca gagactatac tccatttaca
     8461 tttatcagtt agagtgtaag aacgccattc ttttaaagct tttccagatg ccttctgaaa
     8521 atttgcaact ctttcagggt caacatcttt taataaatct ggattttggg catagactga
     8581 taaaaatgcc ccaccttctt ttgctatatc tacatattgt tctgctgtcc atttggggaa
     8641 ctcatgaaat gcttcatctg gagcatataa gtatttaata agagtacttt cttcatcaga
     8701 ccactctata taaacatttt tagcaccaca tttatatgct tctttaagtg catttcttgc
     8761 aaaatcagca cattctattg gtgactttat aagtagaatt tgacctggct gtatgttaac
     8821 tccaactttt attgctaatt cagcgtattt ttctaaattt ctatttaaat tcatatttat
     8881 cctcctatat ttttaatatt gaataactta taaagtatta taacattact tatgttttta
     8941 atattgaaca acttataaaa tattataact ttacttagat tttgggtcat attcaaaata
     9001 tttatacttt atagattgag aaataaaaaa atattttaat taaaacagat tatgaggata
     9061 acaaacttat ttaagcagta tgttttttgt tgataataat attaaagtag aaataaaaat
     9121 acttttttag tgaaaagcta tacaagaata ccgaaaaaaa cttataatat aataatgaga
     9181 tagatattta taaatgaaaa aaaatttttc tggcaaagct taattaaaaa ggtaatagct
     9241 agtgttgtta tttaaattag catagaaaat caaataaaaa aattatgcta atgtatagac
     9301 attgataagg tagaatttaa gccttttgaa tgctatatta aactagagta gatattctta
     9361 tgaatttcaa aatagggcag gaggaaaaag gaaataaaac tataaaaaat ataaagtatt
     9421 tatataaatt tatagagttt atacgtcaga agatttcagt taggagggca taaatggagc
     9481 tttatgaagc cattttttat agaaaatctg taagaaattt ttccagtaaa aagataaaaa
     9541 agccattgat ggaagaaata aaaagaagtt gtaaaaattt agaatactta aatgaagatt
     9601 taaatattaa agcacatgtt gttgatagag gtcatataat acattttttg atgggtaagg
     9661 aatgtaaagt aaaggcacct cattatatag tagttacatc agacaaaggt gaggactatt
     9721 tacagaatat aggatttgct acagaaggtg tagttttgcg ccttacaaca ttgggaatag
     9781 ctacttgctg gttggaagga aacttaaaga gagaagatat cttggaattt gtagatattg
     9841 ggaataagaa ttttgaagaa gatgaggaag atgatttaga ttatctagct ggaataaaaa
     9901 cagaagaaga tgatgaaaac aaaatagaaa atccttatat aataatagca tttggatatc
     9961 cagcaaaaaa tgaagagttg tttaggacga gatatgctag acctgataga aagccagtaa
    10021 aagatatgat aaataagata aataggaata gaaagtggaa agatatctta gaattgacaa
    10081 gaagagctcc atctgtaaaa aattctcaac cgtggtattt ccataaggat gaacgaggtc
    10141 ttcatttatt tgaaaagaga cctaagaaac actgtgaaga tatgaataag gttagcttag
    10201 gtgtagcctt aagacatttt gatatagctt gtataaaaaa taaaatagat gtttcatatg
    10261 aaaaacttcc tataaggaat aaaataggaa aatcatattt tataactgta gtcgagcatg
    10321 tgaaaccaga agaagaaacc caagaagaaa atgtaactct agaaaaagaa gaatcgcaag
    10381 atgaataaaa ttttggaagg ggatattgga tatgtataag tgtggaaaat gtaatcttag
    10441 aggctgttct aatggaaata tggataaatt aatgaagata tgtccaacta gagaaattga
    10501 actacaacaa aaagcaaagc aactttatga agaagaagag aatagaaaaa tagcttataa
    10561 tgctggatta gttgaagcag gggggtattg tcaatatact agactaaaag agattataga
    10621 gtttgctaaa aagtgtggtt ataaaaagat tggcatagca ttttgtattg gactaaaaag
    10681 agaaatgcaa acagtgcaaa aaatatttga acatcatggg tttgaagtat tttctgttat
    10741 atgtaaaaat ggggcaatgc ctaagagtac aattggacta gaagcatctc aaacaatatc
    10801 aaagtgttca gatgaggtaa tgtgcaatcc aataggacaa gctttatttt taaatgaagc
    10861 taaggttgac cttaatgtaa ttcttggatt atgtgttggt cacgatactt tagccatgaa
    10921 atatatggaa gctcctataa caactttagt tgtcaaagat agagtaactg ggcataatcc
    10981 agttgctgca atatataatg cagaatcata ttttgagaaa aaattgtttg ataaagatga
    11041 agaataattc aaagacttat tgacataaaa agcaattagt ggtttaatta taaaaaagat
    11101 aaaactttaa attatcctat agatacataa agttatataa ataaagaaaa atattacagc
    11161 taaattcctt aaagtagtcc agagagacta aagggttata gataatgtta gtatataacc
    11221 tctagtaaat cttctagagg tttttgatga gtataaaata aaaatttaaa tataataaag
    11281 tcttttaaac tagtccagag agactgggaa agatatctca tagctaatga atagagttat
    11341 gtatagataa aatccttatg cctcttttgg tgtgaggatt tttttatgat tatttaggct
    11401 ttattaaaaa ggagatgtaa aaatgataaa tggtaaagaa aaattgatag ttgcaattga
    11461 tactgacgaa tttgataaag ctaaagaact aatagacaga ttagaagata gtgtagacat
    11521 atttaaggta gggctagagc aatacgttgc cacaaaaggt aaaacaatag attatcttaa
    11581 agaaaaaggg aaaaaaatat ttttagacct taaattccat gatataccaa atactatgaa
    11641 aagtgcagtg aaagctgcag tgagagataa tgtttggtta atgacaatac atgtatcaga
    11701 tatggaagga atgagacaat gtgcactaat tgctagggaa gaagcagaaa gattaaatat
    11761 agaaaagcca ttagtagttg gagtaacagt tttaacttca ttaagcaacc aagatttaca
    11821 ggacatagga tgtaatatga caacagaaga gttagctata aagagagcta aactggcaaa
    11881 agaatcagga atagatgggg ttgtttgttc agcgcaagaa gtagataaaa tagtagaagc
    11941 atgtggaact gattttataa cagtttgccc aggaataaga cctaaatcag cagaggttgg
    12001 agaccaaaaa agagttgtaa ctccatcaga tgcaataaaa aaaggtgctc attatttagt
    12061 tgttggaaga cctataacaa aagcagaaaa tccaagagaa tcagctatta atattgtaaa
    12121 agaaatagaa aatgcttaga taaaatattt gggaggtagc ttatatgaag gggaaattaa
    12181 ttttagaaga tggaacagta tttgaaggaa aagcatttgg atatctaaag gaagctattg
    12241 gagaagttgt atttaatact tcaatggctg ggtatggaga actattgaca gatgcaactt
    12301 attatggaca aatagttact atgacttatc cacttatagg aaactatgga ataaatttag
    12361 aagatgttga atcaaataag gtgcaagtaa aaggacttat tgtaagagaa aaaagtgata
    12421 acccaaataa ttttagatgt gaaatggata tagacacata tctaaagcaa aataaggtaa
    12481 taggacttga aggaatagat acaagagcat taactaaaat tattagaaac aatggaacta
    12541 agagaggaat tataacttta gaaaatgtat cattacaaga tgttcagtct aaattagaag
    12601 agcattgtaa caaagattca gtaaaaaatg ttacaagaaa agaaattgaa aatataaaag
    12661 gtactggagc taaagttgta gttatagact ttggtgttaa aaatagtgta ctagagtctc
    12721 ttaaggcttg taactgtgat ataactatag taccagcaat gactacagta gaagaaattt
    12781 taaatataaa tcctgactta atcttattat ctaatggccc aggaaatcca gaggatataa
    12841 atgatatagt tgaaaatata aaaaaattaa taggcaaaaa gccattgaca ggaataggtt
    12901 taggacatca aattttagca ttggcattag gaggaaagac ttcaaagctt gtatttggac
    12961 atagaggagg taatcaccca gttaaatgtt tagaaactgg aaaggtattt ataacttctc
    13021 aaaatcatgg atataatgtt tcagaaatgc cagaaaatac agaagtaact cacataaatt
    13081 taaatgacaa tactatagaa ggaatgagac ataaggaatt accaatatat agtgttcagt
    13141 acattccaga atacacaaca aaagaagatt tagattgtat atttaataaa ttcttagcat
    13201 taatatagat ttagtattat ttacagatag taaaaaccat ttagataagg agaaaaattt
    13261 atgcctaaat tagatagtat aaagaaaact ttagtattag ggtcaggacc aataataata
    13321 ggtcaagctg cagagtttga ctattcagga acacaagctt gccagtcttt gaaagaggaa
    13381 gggattgaag ttgtattaat aaatagtaat ccagccacta taatgactga taaagaagtg
    13441 gcagataaaa tatatataga accattaaca atagaattta tagaaaaaat aatagaaaaa
    13501 gaaagaccag acagtttact tgcaggtatg ggtggacaaa cagggctaaa cctagcagtt
    13561 gaactttatg aaaaaggcat actagataaa tatggagtaa agataatagg tacttcagta
    13621 gaatctatca aaaaaggtga agatagagat atatttagag aagtaatgaa agaaataaat
    13681 cagcctgtaa tagttagtga tatagttaca gatttacaag caggtcttga ttatgcactt
    13741 acaattggtt atccagtagt tgtaagacca gcatacacac taggtggaac tggtgggggt
    13801 atagctgata atgaggaaga attaagagaa atactttcac atggtctaca attaagtcct
    13861 gttggacaag tattaattga aaagagtata aaaggttgga aagaaataga atatgaggta
    13921 ataagagatt caaagggaaa ttgtatagtt gtatgtaata tggaaaatat agacccagtc
    13981 ggagttcata caggagacag tatagttgtt gctccaactc aaactttaag taacaaagaa
    14041 tgtgcaatgc ttaaaaaagc atctttagat atattaaatg cagtagaagt tcaaggtggt
    14101 tgtaacgttc aatttgcact taatccacat agttttgaat atgcagttat agagataaat
    14161 ccaagagtta gtagatcatc agctttagca tcaaaagcta caggatatcc aatagctaaa
    14221 gtttcgtcaa aaatagcttt aggttatact ttagatgaga tagaaaatgc agtaactaaa
    14281 aaaacttatg cttgttttga accaactata gattatgtgg tggcaaaaat accaaaatgg
    14341 ccttttgata aatttaaaaa agcaaataga aaattaggaa ctaagatgat ggcaactggt
    14401 gaaattatga gtataggaag taattttgag gctgctattc ttaaagggat aagatcactt
    14461 gaaacaggaa aatattcatt agttcacaca ccatctgagg agagaagcat tgaggaatta
    14521 aagaaaagag ttgtagtacc tgatgatgag agactatttg atttagctga aatgataaga
    14581 agaggctata aagtagaaat gatagaacaa attacaggta tagataaatg gtttataaat
    14641 aaatttaaat ggatagtaga gcaagaagaa aaattaaaag ttatgaaaat tgaagattta
    14701 actaaagact atcttcttga attaaagaaa aagggattct ctgataaagg tatatctgac
    14761 ctaatgaaga taagtccaga aaaattatat gaattgagaa gtctatacaa tataaaacca
    14821 gcatataaaa tggttgatac ttgtgcaggt gagatagatg ctatatctcc ttactattat
    14881 tcaacttatg agcaatatga tgaggtagtt gttagtgata agaaaaaggt gatagtatta
    14941 ggttctggtc caataagaat aggtcaaggt atagaatttg actattgttc agttcactgt
    15001 gtaaaatctt taagaaaaat ggacatagaa actataatca taaacaataa ccctgaaact
    15061 gtaagtacag actttgatac atcagataag ctgtattttg aaccattaac agaagaagaa
    15121 gttttaagta taatagaaaa agaaaagcca gaaggtgtaa tactacagtt tggtggacaa
    15181 acagctataa aattggctaa gtttttacat gaaaaaaata tacctatact gggaacagat
    15241 tttgatgata tagatgcagc agaagataga gaaaaatttg atgatttact agagagacta
    15301 gatataaata gaccaaaagg aaagggtgta tggtcact
//
LOCUS       DAMJYC010000095        15162 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJYC010000095 DAMJYC010000000
VERSION     DAMJYC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.099"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            68..1909
                     /locus_tag="PC472_003113"
     CDS             68..1909
                     /locus_tag="PC472_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2728422.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            2214..2921
                     /locus_tag="PC472_003114"
     CDS             2214..2921
                     /locus_tag="PC472_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728423.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            2915..4225
                     /locus_tag="PC472_003115"
     CDS             2915..4225
                     /locus_tag="PC472_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728424.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            4342..5025
                     /locus_tag="PC472_003116"
     CDS             4342..5025
                     /locus_tag="PC472_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728425.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            5018..7405
                     /locus_tag="PC472_003117"
     CDS             5018..7405
                     /locus_tag="PC472_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2728426.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            7685..8827
                     /locus_tag="PC472_003118"
     CDS             7685..8827
                     /locus_tag="PC472_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2728427.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            8846..9370
                     /locus_tag="PC472_003119"
     CDS             8846..9370
                     /locus_tag="PC472_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2728428.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIIFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKNCIK"
     gene            9470..10288
                     /locus_tag="PC472_003120"
     CDS             9470..10288
                     /locus_tag="PC472_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2728429.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEVVSQLFAKKLEESLSPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            10366..10677
                     /locus_tag="PC472_003121"
     CDS             10366..10677
                     /locus_tag="PC472_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2728430.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(10854..11537)
                     /locus_tag="PC472_003122"
     CDS             complement(10854..11537)
                     /locus_tag="PC472_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728431.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIHNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(11630..12271)
                     /locus_tag="PC472_003123"
     CDS             complement(11630..12271)
                     /locus_tag="PC472_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728432.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(12445..14448)
                     /locus_tag="PC472_003124"
     CDS             complement(12445..14448)
                     /locus_tag="PC472_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2728433.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(14601..>15162)
                     /gene="recX"
                     /locus_tag="PC472_003125"
     CDS             complement(14601..>15162)
                     /gene="recX"
                     /locus_tag="PC472_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2728434.1"
                     /translation="IAIFKELVYTFNLKKGNEINEEELKPILDDEMYIKAKNKALNIL
                     SHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVNDSVLAQKIVNTNVNLNKCGKN
                     RIKQNLYNKGINRSTINEAVSELDKDTEFENAMYLAKKRYERVKKEDKKKIYQKISQH
                     LSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT         5864 a         1721 c         2447 g         5130 t
ORIGIN      
        1 aaaaagtttt atgtaaaata taaattttat attaaagcta aaactaattt gaggaggcta
       61 atttatattg aagattagaa ggtatttaat tttggtaatt ataattctaa ttacaacaag
      121 tcagagtttt atttcttatg ggctagagcg atttgaagaa ataaccattt ttcacactaa
      181 tgatatacat ggaagatatg ctgaaggtga tgaccatata caaataggaa atctagccac
      241 attgaaaaaa gaaactccaa actcaatttt agtagatgca ggagattgtt tgcatggact
      301 tccaatagta aatatggata gagggaaaag tgcaattgaa ttaattaaat gtgctggata
      361 tgactatatt actcctggaa atcatgactt taattatgga aaagatagac tgtttgagtt
      421 atctaaaagt gctaatttag gagagaatac tttaaaattt ttatcatcaa atatttttga
      481 aaatggaaaa aaagtttttg atagtaatga tataaaagaa atagatggaa taaaaatagg
      541 attttttgga ctctcaaccc aggaaacaaa gagcaaaact ggatttaaaa atgtggaagg
      601 attagagttt aaaaatccta tagaaagtgc aacagaacaa gtaaatgaat taaaaggaaa
      661 gggtgccaat gtaatagttg caatatgtca tattggtaca aatagtttaa gtaagccaaa
      721 tagtatagat atagcaaatg aggttcaagg aattgatttg attattgatg gacacagcca
      781 cactaagttt gaaaatggta aacaagtggg tgatacattg attgtaagtg caggacaata
      841 tttagaaaat attggcgaga taaaactaac tttggatact aatgatgtag aaaatataaa
      901 aatagaaaat aaatcagtta gattaattgg aaaagaagaa gcattaaagt ataagcttga
      961 ctccgctgta agtaaaaagg ttgatgaaat aaaagaacaa caagaaaaaa tattagataa
     1021 agttataggg gtaactacaa atactctaga tggttcatat gaaaatgtaa ggacaaaaga
     1081 aactaatttg ggtaatttaa taagtgacat tttactagat aaaactaagg ctgatatttc
     1141 tttatttaat ggagggaata ttcgagatac gatagaaaaa ggcaatataa caagaagaga
     1201 tattgtagat gtatttcctt ttagcaatac tatagttacc aaagaactta caggagcaca
     1261 gattaaagat gtattggagc atggagtaaa actttatcca gaaaaaagtt cagccttttt
     1321 gcaagtggga ggaatttcat attactttaa tcctaaacaa aaagaaggcg aaaaaataac
     1381 aaatattcaa aaagaaggaa agccattaga tttaaataaa aagtatgtag ttgcaacgaa
     1441 tgattatata gcatcaggtg gagatgaatt tccttgtttt tcaaaagagc cagtactaaa
     1501 ggaatttgga aatttagaga gtgcagtcat tgaatatata gagtacaaaa aagaaatatc
     1561 aaaaaatgta gatggaagaa ttggtattaa atctaaagat gagttaataa gcaagaatca
     1621 tgagagtgat ttgatattaa gtgaaaatga aaaaaatatc aatgataatg aagagaatct
     1681 atctaataat ataaaaagct ctgatagtga taaaaataaa agcttagata aaacaagtgc
     1741 tagagaaagt tctaatctag gaatatctaa aagagaagct aaagaaaaat ctcctaaaac
     1801 aggagattta ggtttttcaa atagtataat tatatttata gtttcaagta cattgatatg
     1861 tttattgaat tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa aattaatgag
     1921 caaaaattag tagatagtaa tttaatttta gataatagta aacagcaaag gaaattagtt
     1981 atataaatac taatttcctt tgttttatgt tttaacatga ttacttttaa ataaaattgt
     2041 attttatgaa aaataattac actaaatggc atagtattat ggggattttt agtttttatt
     2101 caagtaaatg aaatcatatt aacaacttct gatatattta caaattgata aaaaaatgat
     2161 tttattagta tgatataatt tagctagtat atatgatgat ggaggatata gggatgaata
     2221 atatattact tttggaagat gacaaatctt taaatagagg aattagtttt aaattaaaaa
     2281 aagaaggata caatgttttt agtgcatttg gtatagaaga agcaaaaaat attttcgcaa
     2341 aagaagagat atgcttaatt atttccgata taggtctacc tgatggtagt ggttttgact
     2401 tttgtgagga gattagaaag aaaagtgatg tttacataat aatgttgaca gctcttgatg
     2461 aagaggtgga tattgtcaca ggatatgatt taggtgcaga tgattatatc acaaagcctt
     2521 ttagcttgat ggtattaata tcaaaagtga atgcgcttat gaaaagagta aatacagtaa
     2581 aaaattatac tctattagtt tgtgatgatt tattctttta ttatatagaa aataaattaa
     2641 ttataagaac agatgataag gaagaagaga taattttaag taagacagag acaaaactat
     2701 taaaatattt gatggaaaat tctatgcaga cattgactaa agagcaactg ttagaatctt
     2761 tatgggatag tagtggtaat tttgtagatg ataatacgat tgcagtaaat ataagaagac
     2821 ttagacaaaa ggttgaaaaa aatccatctg aacctaaata tataaaaaca gtaagagggg
     2881 ttggatatat gtggggagaa aggagtataa aaaaatgcta aaaaatatga acctcaaaga
     2941 tggtttaagt gtgaaagatg attatataat gagaagagtt attgtatatt ctgttttatc
     3001 tgtactaata gtaacccttg tatttatact ttttgaatat agcagattac ataaattgta
     3061 tgaacatcaa ataaaaaata ctcaaaatat tgctggaata ttgattgaaa aatatcctaa
     3121 tgatgaaata gatatagtaa attctattta taattctgat tatagtaagg tgtctactgg
     3181 agaaaaggca tttaagaaat ttggctatgg attggaaaat aaaatgtcaa atgataaaaa
     3241 ttttagctta tacttaaaat ctttttttaa ggaatcattt tttatttttt taatgatgtt
     3301 tatagtagtt ataggtataa tatattattt tataagatac ataaataaaa gattgagtaa
     3361 aatttatttt atagtagata agatgtcaca agaaaactac ttgaaggaca ctactgagtc
     3421 agaagaaact ggttatctag tagaatataa tgaatacttt aaagaaggca tatttagtaa
     3481 gataaataac tgtctatatg agcttaatag aagtttaaag ataaagttta ttaaattaga
     3541 aaaagaaaaa gaaagtgtaa aatcattagt aacagatatt tcccatcaat taaaaactcc
     3601 tttagcatcc ttaaaattat acaatacatt acttatagaa gaagaattag aagaagatga
     3661 agccaacgag ttcttattaa ccaataaggc ttctataaat aaattagaaa atttaataaa
     3721 ttctttagtg aacatatcaa ggttagaaat tagcatgata aacataaaaa aagaagataa
     3781 tgatataaaa aatactattc taaatgcagt aaaaagtgtt acccctaagg ctaagagcaa
     3841 aagtataaca ttaaatttaa atgaatttaa cagtagaatt ataccacatg ataaaaagtg
     3901 gactgaggag agtattttta atgtattaga caatgctgta aagtatacac aggtaaatgg
     3961 agaaataaat atatctgtag aagaaactgc taatttcttt aaaattataa tagaggataa
     4021 tggcataggg attgaaaaaa atgagtttaa taatatattt aaacgttttt atagaggttc
     4081 tgctgatgaa attgaatctg tagaaggtag tggggtagga ttgtatttaa gtagaaagat
     4141 tttagaagaa caaggtggaa atattatagt ttcttcaaaa gtaggaatag ggagcaagtt
     4201 ttctttgttt cttacaacta tgtaatatta aatgaaagga aaatgtaagc ctctacattt
     4261 attatataag tataaacagc tccagaaata aaaaattaat aaaatatgga gtaatataac
     4321 ttagaatgag aggataattt tatgaaaata ctatatactg aaaatttatc aaagcattat
     4381 ggaaaaggag aaagcttagt aagagctttg gacaatgttg acttagaaat aaatgaaggt
     4441 gaatttgttg caataatagg aaaatcaggt tctggaaaaa gtacactttt gcatatgatt
     4501 ggaggtcttg atatacctac atctgggaag gtgtatatag ataataaaaa tatatttaca
     4561 ttaaaagaag aagaacttgc tgtatttaga agaagaaaaa tagggtttat atttcaaagc
     4621 tataatttaa ttccatctct taatgtatgg gaaaatgtag tgttgccaat cggtttagat
     4681 ggtagagaag tagatgaatc ttttataaaa gaacttttaa aatcattagg acttgaaaat
     4741 aaacacgacg ttttaccaaa tacattatca ggtggtcaac aacaaagggt ggcaatagca
     4801 agagcattag ctacaagacc agctataata cttgctgatg aaccaactgg aaacctagat
     4861 tctaaaacgt ctgatgaggt tatgagtata ttaaaatcta tgtctaagaa gtacagtcaa
     4921 actctagtta tgataactca tgatgacagt atagcccaaa tggctgatag agttatattc
     4981 atagaagatg gcagggtgtc aaaggttggt gataaaaatg attaatacta ctaaaatagc
     5041 aaaaagtaat ttaaagcaaa ataaatcaaa aagtattcta ataataatta ctataatact
     5101 ttcaacctta atgctttctt ctattggaat ttatattgtt gatgcaggag catatcaaaa
     5161 agaaaacact ataaagtatt caggtaatta tcaaggtata ttagcaaatg tagatgaaaa
     5221 acaggctgat atattgagta atcatgcaga tgttgaatta actggtgaaa tgaatggagt
     5281 aggtgttgaa aaactggaag atgattcaaa tatttctcta gcatacatga atgaagatgc
     5341 tttgaaatta aatagttttg aatttatcaa aggaaaattg ccatcaaaag agaatgaaat
     5401 tgtgttagat agtggagctt taaaggcact tggttataaa aataaagatt taggtgaaaa
     5461 gataaaaata tcttatgatg actataaaaa tgataaaaaa attgaaaaag agttcattat
     5521 aagtggaatc ttaaaaacta gcgaaatatc tgaagcaggt aaatattatt atgctattat
     5581 atcagaatca tatatgagaa atactagaga tatgtcacaa gaagatttta atatttatgt
     5641 gaaatttaat gataaaagta atttatcaat agagcaagca aaagaaaaat tagataaaat
     5701 tgctaatgat ataggactgg atactattaa tactgctgta aatgaaaatt atataaatgc
     5761 tttaaaacca gatatggaaa cgattatggg tggagttttt gtagggttag taattgtatt
     5821 atctagtatt ttagtaatat acaatatatt ttatatttca atagttacaa aggttcaaga
     5881 atttggaaaa cttagagcaa ttggtgcaac taagaaacaa ataaaaaata tagtgtttaa
     5941 agaagggttc atattagccg gaatagcaat accaatagga ataattttag gctatgtact
     6001 tgcaaatatt ataataaaat catttatgga tatagatgta aaatcatcac gattaccagt
     6061 tatattatcg gttgtggtaa taagctttat atctgttgtt ttatcacttt taaaaccaat
     6121 gaaagtagca tcaaaagtat ccattgttga cgcagtaaga tatacaggaa ataaaataag
     6181 taataagagt aagagaaaag gatataaaaa tataaatcta aagagattat cacatgccaa
     6241 tttagaaaga aataaaaaac gtacttacat gacattggca tcacttatac ttagtggaac
     6301 tatatttata acagtatcaa ctgccttaga aagttttgat gctgagaaaa tggcaagaga
     6361 acactttcct tatgatatag aagttagact atctggttat gaaatgaaca gtgataaaaa
     6421 tcctaaagat aatttgaata ttttacaaat ggataatcct ctaagtaaag atttttttaa
     6481 tcaaataaag aacatggaag gaattaaaag aatagaatct gcaagaagtg taaaaattgg
     6541 tatggaggac tatgatgttg aatttaagta tgacctttta caaagtataa atgaaaatga
     6601 tgtaaaatca ttaaacaaga atataataga tggaaaaata aatcttgaaa gacttcaaac
     6661 tggagatgaa atagtaatta ctcatgttga tacagcaaaa gaaatgggag taaaagcagg
     6721 agataaaata agattaactt tatatgatgg agataaaaag ataaaaaaag aatttaaagt
     6781 tcaagcaata gctatggggg ttccaagttt tggtataggg aaagatttta tagatagaac
     6841 tttgaaatac gattcaacta gtagcttggg aatatataca gaagaaggaa agtatcaaga
     6901 aatcaaagat agtatcaaga agattgctaa aaataatgga tttttagaga ctgactttat
     6961 agatagtaga attgagtcaa ataaggctac tatttcattt ataaagataa tgggatatac
     7021 gctaacaggc ataataggtg tgattggatt tatgaatctt gtcaacacaa tgataactag
     7081 catagttact agaaaaaaag aacttggtat gcttcaagct attggtctta ctaataaaca
     7141 attagtgaaa atgcttaatt cagaggcaat gtattacact tctggaatga tgataggaag
     7201 tattctattt ggaggtatat tagggtatat tgctgttata tttctaaaaa agactggatt
     7261 atcttatgca acatattctt tgccaatagt accaatcttg ttgatgattg tgtgtattct
     7321 tatagctcaa tttataacaa cttatttgat aggacgaagt tttaataaag aatctcttat
     7381 tgatagagta agatacagtg aataaaatgc aaatatttat ggttagttta actagcaaaa
     7441 aacatataaa aaaatatagt taaagaaatt cataattaaa taggttaaag catagtacag
     7501 agcaaacaat ttaaacagat gtttgctctg tattttatta tgtaatcaat aatatatcta
     7561 atatactgat attattcatg ttagaaaatg taccggtaca attctaaaaa agtataaaat
     7621 tgtaccatat aaaatgaaaa ttttgtataa taatcgtata gaaaataaat aggagctgat
     7681 atatatgaat aagaagatta attatgcacc aggaccaact gaaactaggg aaaatgtaag
     7741 attagcaaga gcagaaaaaa caacaaatcc agatatagat attgattttg ttgaatttta
     7801 taaaaagaca tgcgagaagt ttggtaatat aattggaaca aaaaacgatg tttacatatt
     7861 gagtggtgaa ggaatattag gtcttgaagc agcatgtgca tcacttacag aaaaaggaga
     7921 tagagtactt gtaattgata atggtatata tggagaaggt tttaaagatt ttgtaacaat
     7981 gtatggtggg gagtatgtgc ttttttcaag tgaatatact aaaagtatag atattgatga
     8041 attgaaagca tttttagatg aggatagtaa ttttaaatat gcaacagtag tgcattgtga
     8101 tactccaact ggcgtattaa atgatgtaag caaaatatgt ccattactta aaaaatatgg
     8161 aatattaact gtagttgatt ctgtagcagg tatggtagga gaaagattaa gtgtagatga
     8221 atctaaaata gatataatat taggtggttc acaaaaagct atttctgcac cagcaggact
     8281 tactatagtc ggaataagtc aagatgcaaa aaattgtata aaaaatagaa aaactgatgt
     8341 aataggtttt tattgtaatt taaatatatg ggaaggctat tatgaaaaaa aatacttccc
     8401 ttatacaatg ccaataagtg acataatggg gcttgataaa gcacttgata atatattaga
     8461 agaaggaata gaaaatgtat taaatagaca tgaaaagata gcttatagtg ttagaaaatc
     8521 tattgaggaa tatggtctag aactattttt agaagaagga tattctaata cagtcacagc
     8581 tataaaagtt cctgaaagta taggagcttt agaacttact gattatatgc ttaaaaatta
     8641 taatacactt gttgcaacat cactaaatca atatatggat actattttaa gaataggtca
     8701 tatgggagag aatgcaaatc taaataaaat agaacatgta ctaaatgttt tggataaaag
     8761 tttaagtgca ctaggattca aaggaaatgg aaatttgtta aatttattta ataagtatta
     8821 tttttaatag atagggggat atagtatgga agttacagct aagcaaaaat ttacaacgaa
     8881 agacatggtg gaaacctcac tattaatagc attagtattt attgcaacta aatttataaa
     8941 cataagatta ccaatatcta taaatggtgg gcttgtgcat ttaggaactg caatgttgtt
     9001 catatcagca ataatatttg gaggtaaaaa aggtgcttta tcaggggcta ttggtatgtc
     9061 actttttgat ttgatatctg ggtggacttt atgggcaccg tttaccttta tagttagagg
     9121 agttatgggt tatttacttg gaaagattgc atgggcaaat ggtaataatg ggaataattt
     9181 tttaatcaat gtaataggaa tatgtgtctc atcaatttgg atgctatttg gatattatgt
     9241 aacagaagtt atactttatg gtaattttat agtacctctt acttcaatac caggtaattt
     9301 aatgcaagta ttgataggtc ttataatagc gcttcctatt tctaaggtat tgaaaaactg
     9361 tataaaataa atataaagtg aactaaaatt tatgatagca taaattatac tgtataatat
     9421 taggatggag tacataatta aaaaatttta gtatagaggt tagttcacaa tgttatcagt
     9481 tacaagatat tcaatcaaaa atgttcgttt aaagccgtgg gtaaagttta tttggtattt
     9541 tgagacaaaa tcaaatatac tgttaaataa taagttactt ccgacagaca gtatagatat
     9601 tattctcaat ctttctgatg ttatggaata taaaattgaa aatcaagact atactgctag
     9661 taatatgcat tttaatggaa taagggataa acatggattt attattcaac atggaaatat
     9721 acgtgttata gggatttctt tttatccatt tggtttatat ccatttttaa agataccaat
     9781 ttcagagttt aacagacaaa ttgttgactt agaagtagta tcacagctat ttgcaaaaaa
     9841 attggaagag tcattaagtc caacacaatc agtagaaaaa attgttttat atttagaaga
     9901 agtattatta tccatattag aagaagattt aatttcaaat aaatatgtaa agcttctaaa
     9961 ttcatttata tatgttaata gatatagcaa tataaaggca ttttgtgatg atacaaatat
    10021 aaatattaaa acattggaaa gaatctgttt aaaatataca gggtatacac ctaagatttt
    10081 aaagagaatt tacagattta aaatggcaag taatcaactt atttataatt ataaagatga
    10141 tgaacttttt gattttattt atgaaaatga gtactatgac caagcacact ttataaaaga
    10201 atttaaaaaa ttttcaggga cgtcaccaat taaatttatt ggtgagaata agacgattaa
    10261 agaaaatata aagtacagct atttataatt atactgtcga ttttttacaa tacctccaat
    10321 ttattttttg atatgatata ggaaaataaa ttggaggttt tacatatgaa aattattaca
    10381 gaaatagttg agtttaatgt ggaagaaaat ttatcaaaag aagagtttat agaaattgta
    10441 aatgacttag aattaaaatt tcattctatt caatctggtt ttattgatac tgagctttta
    10501 tatgatgaaa agtctcaaaa gtggattatg atacaacatt gggaatctat agaagacctc
    10561 aagagtgctt ctagtaaaat gtttaaagat tcttcaactg agaaatttag aaaagcttta
    10621 aagaatcaaa ctgttaagat gaatataata ccacaaatta aaagttggaa attatagaac
    10681 tgaatgtaaa agatagttaa atagtatcaa actaaattag ccaaataaca ttttgtattt
    10741 aaattgttat ttggctttta gtgtttattt taatatgagt gttatatact agaaaaacca
    10801 acaaactgtt agagtgtgga ttatcataat gtcatattcg ggagctaaaa gtcttaatac
    10861 tgatttgcca attggtcaat ccatgcatgt ccatctggat atgctgttat taaagcattt
    10921 acaagccaat ccctttgctc ttgtgataat agtgggactg ttacatcgaa gtctttttga
    10981 taaccaagcc tatctatgta tattgatttg caaaacaaaa ccacttcggg agccatatat
    11041 gggatattat ctgatgttat cagtctggcc ttatccatat ctcttataat tctttgattt
    11101 tgggaaaaaa gaaattttcc atctttctgt ggattatgta tcaattcaat aaaatcgaac
    11161 tgtaactgtt cgtcacactg tatattcatt ttatagttat catcctcgac tttttttaat
    11221 tcaataaatg agcaatcagg cttaatcgca aaaacacaaa tttgctctat atgttgcttg
    11281 ttaaggtcga taataggtcg taataaataa ttacccaagg gctcatatat atcccatcct
    11341 tgattaagca tatactgaat gctcttttct ttatctgtgt cagaaatcca tatgtcaata
    11401 tcactgtgtg ggcgaatatg catattcgca aataattcta gggcatatcc accacatatt
    11461 gaatatgaaa agccacaatc attaaaaaag tttacacaat ctttaattag tttgttcata
    11521 gcaacctcct tttgcatgta ctgaaataaa ttaaattttt aaaaatacca actaaatgtt
    11581 tgttttgtgt aaatataaaa ctgccgatat taaggcagtt ttatatttac tatttagaaa
    11641 atttattaac aaatttttga tattcacaca ttatcttatc attcataaaa gaaataccca
    11701 taaaagcagc ttgctcacca aacaaagtaa aacgttctat catctgttgt ggattacctg
    11761 gataaactgt tggaatacac catgtgttta gaagaaaacc aaacatttga gcagcaatct
    11821 ctggattttt aacttccata gaattatctt gatttccttc ttcaatcaat gcttttatcc
    11881 aaggcacaat atcatttata atctcatcaa actgcgtagc taaatactta ggattacgca
    11941 atagtgatga tgacattaaa aaaatgtctt gtttattgtg ttgttcataa gcatgaagca
    12001 acctatattg taatttttct aatgcggtct ttccttcatc attttttacc tcatacccat
    12061 aaaatccatc attatacaac cgacttgtga ttgccttaat tatttcgtct tttgatttaa
    12121 agtgatgata aatagcacct cttgaaaggc catctaaatt atctacaatg tcttgaatcg
    12181 ttgtctgctc ataccctttt tctaagaata acttatgtga aatatcaagt attttttgta
    12241 tagtattatc aggttctttt tttcttccca aaaagctcac tcctcaaaac aatcatttgg
    12301 tatgtttatc ttagcaaata cataatgatt tgtcaatgtg tctttttagc atgttgaaca
    12361 ggtatattgc catgctttca ttattttatt tttttgcctt atactggact atatttgcag
    12421 tttcctaata tatttggctc atatttaaat accattatct aaaagcacta ttgctgtacg
    12481 agaggcaata ggcattttag aagaaggagg agttgagtta aagtatacat cataatctct
    12541 taagttttct ttttttgtgt ttcctccaaa ctctaccata tcagtattta atatcgtttt
    12601 gtagtttgta ttttgaaact ttgaaatatc aactgattgt attagattag ggtggaaatt
    12661 aaatataaat aaatatttgc ccttactgta caccaaaatc tttttatcat tatccatatg
    12721 tataagttca tgactatttt ggataaaaat atcactgtat tttgtaagtt cgagcataga
    12781 tttatcaaaa tttaataatt gtttatactt taagttgtta tcttctgaaa ggctccattg
    12841 tcttctggca tacttatagc tccaattgtt gccttctcgt ggaaaatcaa tccactcagg
    12901 atgtccaaat tcatttccca taaaatttag atatccttca cctgctagag aaaaagttat
    12961 taatttaatt aatttaataa gagatattgc tctattgata acatggttat ttgagtttat
    13021 ttccatattc cagtacattt ccttgtcagc tagccaaaaa ataattgttt tatcgccaac
    13081 taatgcttgg tcatgggatt cacaatatcc tatatttttt tctccaggtc gtctagtagt
    13141 aagttcatac cacatttttc ccaaatccca gtcttcatca gataagttag agatagtttt
    13201 tatccaaaaa tcaggtactc ccattgcaag tctatagtca aatccaattc cgccgtcttt
    13261 tatagggata cacataccag gcattccact catatcttct gcaatactta tcgaattagg
    13321 tttaatttct tttattaatt catttgcaaa ttggagatat gttatagctt caatatctgt
    13381 attcatacta aaatattttt cataactatc aaatgaaact ccaagaccat ggttatgata
    13441 taacattgaa gttacaccat caaacctaaa gccatcaaaa tggtactcat ttaaccaaaa
    13501 ttttatattt gaaagcaaaa aatgtattac ttcaggttta ccatagttaa aaagttttgt
    13561 accccaagca ggatgatttc cctttgaacc tgagtggaaa aattgatgtt cacttccatc
    13621 aaactcattt attccttcta aagtattctt tacagcatga gaatgaacta aatctaataa
    13681 aacagcaatc cccattgaat gagcagtgtt tatgaggttt tttaagtctt caggagtacc
    13741 aaacctagat gagattgcat aaaagtttga aacttggtat ccaaaagatg cataatatgg
    13801 atgttccata atagccataa gctgaatggt attgtatcca gcttttttta tcttaggaag
    13861 tattttttca gtaaattcat tataagttcc aatagattcg ctttcagtag acatacctat
    13921 atgacattca tagattagtg gagaagttat gttttttaag tcaaaattat tgtcagtcca
    13981 gataaatgga gtttttggtt gccatatttg tccattaaat ccaccaaaat ctttttgaat
    14041 tactcttttt atatataatg gtatacggtc aaatgttttt ccatttgcag taacttgtac
    14101 ctttacctct gatttgtgag gtaagctatt ttgaccagga ataaatattt cccaattacc
    14161 agacccaatt tttcttagag gatgagattt tctattccaa ttattaaagt caccaattag
    14221 tgacaaacta tctgcatttg gagcccattc cctatagaag aatccatcat ttgtctgatg
    14281 aaatccatag taaagatgag catttgcaaa agaactaaaa tctttatagt ttttcaatgt
    14341 ttttgatttc atatcctcat aatgtttcat tcgctttttt atatcagatt caaatggttg
    14401 taggtataca tcaatatcta aaattttaaa tcttttttga cttttcattg tatttctcct
    14461 ccataataaa tattacatct aaaaaataat atttatctca tactatcttc tctgtataat
    14521 tgatatatat tatataatcc taataaaaag cttaagccac caatgattaa tttcataatt
    14581 tacctcccta gtatttatac ttatatatcg tattcatcaa aatttaaaag tttgtttaaa
    14641 actcttttaa taatatcata atcaaagcct ttataggaaa gatgttgaga aattttttga
    14701 tatattttct ttttatcttc tttctttact ctttcatacc ttttttttgc tagatacata
    14761 gcattttcaa attcagtgtc cttgtctaat tcagatactg cttcattgat tgtacttcta
    14821 ttgatacctt tattatataa attctgctta attctgtttt tgccacattt atttaggtta
    14881 acatttgtgt taacaatttt ttgagctaat acactgtcat ttacgagatt ataactttta
    14941 agaaaatcta tgaccctctc aattatattt tcatcaaatt ctgatgatag tttttctttt
    15001 aatttttttt cagattggtc agcatgagat aaaatattta atgctttatt tttagctttt
    15061 atatacattt catcatctaa aataggtttt aattcttctt catttatttc attccccttt
    15121 tttaaattaa atgtataaac aagttcttta aatatagcaa ta
//
LOCUS       DAMJYC010000096        14777 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJYC010000096 DAMJYC010000000
VERSION     DAMJYC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.100"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            109..621
                     /locus_tag="PC472_003126"
     CDS             109..621
                     /locus_tag="PC472_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2728435.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(776..1837)
                     /locus_tag="PC472_003127"
     CDS             complement(776..1837)
                     /locus_tag="PC472_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2728436.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1961..2854)
                     /locus_tag="PC472_003128"
     CDS             complement(1961..2854)
                     /locus_tag="PC472_003128"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2728437.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(2864..4345)
                     /locus_tag="PC472_003129"
     CDS             complement(2864..4345)
                     /locus_tag="PC472_003129"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2728438.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(4453..4684)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5486..6394
                     /gene="trxB"
                     /locus_tag="PC472_003130"
     CDS             5486..6394
                     /gene="trxB"
                     /locus_tag="PC472_003130"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2728439.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(6484..8307)
                     /gene="typA"
                     /locus_tag="PC472_003131"
     CDS             complement(6484..8307)
                     /gene="typA"
                     /locus_tag="PC472_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2728440.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            8519..8761
                     /locus_tag="PC472_003132"
     CDS             8519..8761
                     /locus_tag="PC472_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728441.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            8909..9100
                     /locus_tag="PC472_003133"
     CDS             8909..9100
                     /locus_tag="PC472_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2728442.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            9384..11885
                     /locus_tag="PC472_003134"
     CDS             9384..11885
                     /locus_tag="PC472_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2728443.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            12045..12713
                     /locus_tag="PC472_003135"
     CDS             12045..12713
                     /locus_tag="PC472_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728444.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            12697..13974
                     /locus_tag="PC472_003136"
     CDS             12697..13974
                     /locus_tag="PC472_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2728445.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            14375..14596
                     /locus_tag="PC472_003137"
     CDS             14375..14596
                     /locus_tag="PC472_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728446.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         5648 a         1874 c         2267 g         4988 t
ORIGIN      
        1 aataatatat ttaaaaaagt attttatttt caaaactaga cttcatttat aatttatcat
       61 gaaaataaaa ggaatataaa tataagagaa tgagaggtta ttagagatat gagtgatttt
      121 aaagaaattt tggcagaaga gattaaaaat aatccatttg gattgatagg taaagattgg
      181 acacttataa cagcagaaaa tgaaggtaaa gtgaatacaa tgactgctag ctggggaggg
      241 cttggagtta tgtggggaaa agatgttgct tttgtagtaa taagaccaca gcgttatact
      301 aaggaattta tagataatac agataaattt tctcttacat tttttgatga agattttcgt
      361 aaagagcttt catattgtgg aaaagtatct ggtagagaag aggataagat atcacagatt
      421 ggatttaata tagaacactt aaatgataca ccttatttta aagaatctaa aatggctatt
      481 atatgcaaaa aaatgtatag tcagaaactt gaacctcaat gttttattgc tgaaggtata
      541 gatggaagat ggtatccaca aaaagattat catactttat atatagctga aataaccaat
      601 gtacttgtaa aagaagatta gttataaagg ttaatactat ctctaattaa attaaaattt
      661 ccataaaagg aaattgacac aaaaaagcta aggacataaa tatgtttata aagattaata
      721 aataacgtat ttaaactcct tagcttgata aaattttaat ttagttttct ttaaattata
      781 attcagaaat caattcattg ataacatctt caacagtact taatttatca attttatagg
      841 catcatttcc acaaaataaa agtgaatttt caacatctcc tttaactgca ttgattagag
      901 cttgagaaat acagtaagga gtttcttttg gattacaagg aatcaaacaa ttatagcact
      961 tctttatttc tggacgattt attttaactt cttctataaa tttgtttctt atggcgcgtc
     1021 caggaagacc tacaggactt ttaactatat gaacatcttc ttcactagca tttatgtatg
     1081 ccattttaaa gttctcatgg gcatcacatt cgtaagtagc aacaaatctt gtaccaactt
     1141 gtacaccact tgcacccata tctatatatt ttttaacatc actactagaa gttataccac
     1201 cagcagcaac tactggtatg tttctaccaa atttttctcc ataagtattt gcaaccttta
     1261 aaatttcaac aaattcctta tcatagtcta tagaatctat gttatcaagt tcctcgttag
     1321 agtatccaag gtgaccacca gcttttggac cttcaacaac tattaagtca gcagtagttt
     1381 tctcttttct atcccacatt tttaaaatta cattagcagc ttttgcagtt gaaactatgg
     1441 gtgctatttt tacattactt ccctttacta atgatggtag cttattaggt aatccagctc
     1501 cagatattat aaggtctaca ccagctttaa cagcttcctt tacgtatgtt tcatattctt
     1561 tcatagctac cataaaatta ataccaataa taccaccatt tgagatttct ttggccttag
     1621 aaatatgttt ttttatagct cttaaatttg catttatagt attagtttca aaatcttcct
     1681 catcatatcc aatttgaact ccagaaatta caccaacacc accaagctta gcaactgcac
     1741 cagctagtga tgacctagat ataccaacgc ccattccacc ttgaattata ggaacttttg
     1801 caactaaatc tctaattatt aactctttta tgataatccc tcctcaatgt aaacatgttt
     1861 aaagaactag attataatac ttaatattat tacctagtcc taattattat actacaaatt
     1921 ataataaaag agtagttata ttttgtatag aagacgataa ttatattata tttaaaacag
     1981 ctccattaga atcgcaacat aactctttta tttcccaatc agattttagt ttttgtaggt
     2041 aatttttcat attatccaca aaactatcat cattttcttt tattaaagac attatagtag
     2101 gtccagctcc acttaaaaat actccaatac tatttaattg ttcagatttt tcaacaatat
     2161 cattgtagtt ctcaattaga gttcctctat aatcttgatg caatttgtct tgacaagcta
     2221 ctttaagtag gtcaaagttt ttgtttagta aggcagatat tagtaaagca actctactaa
     2281 cattgaatac cccatcagaa tatggtattt cctttggtaa aacacctcta gctttttctg
     2341 ttgataactt aaaattaggt atcattgcac aaaatttaag ttcttcagga atttttataa
     2401 tatcataatg tatattctca ttatcagtaa cagatacaat catatttcct aaaatagcag
     2461 gagcaacatt atctgggtga ccttctattt ctgaagctat atttagtaat tgatttttat
     2521 ctaaattagc acctgataga gcatttgcag ctataactcc agctactata caagaggcac
     2581 tactgccaag acctctacat ataggtactt cactttggat ttttattttg ataccagcag
     2641 gtattttttc tggtttaact ctatcaaaaa agtacttcat agatgtataa actaagttgt
     2701 tttcgttttt gtaagcatct tcacaacctt ctatttcaag accactttca atttcttcaa
     2761 cataaaattt attgtatata tttaacgcaa ttccaagaca atcaaatcct ggacctatat
     2821 tagcacttgt tgcaggaact ataatctcta acataataat cacctatacc tttaagaatt
     2881 tttttatagc tagttttaat tcatcttttt cataaatatc tttatgtagt atttctgcat
     2941 ttttaagatt tttaataggt tttggaatat ctattttaga aatacttgat aatctatcaa
     3001 ttatagcaaa gtcatcaagg tctttaaaat cacaatctat agactttaga acatcttcag
     3061 aaaacttgaa tggacttgca gtacttacaa taacagtttt tgtgttatca gaagtttctt
     3121 ttttatattt ttcataacaa ttgtatgcaa cagcagtatg agtatctatc aaataattat
     3181 aatttttaaa cacattgttt atcgtgcttt taacttcttc ttcatttgaa tattctccat
     3241 agaatgaggc taaatttttt tccatatcat cacttatttt gtagactcct ttatcactaa
     3301 ggtctgataa aagcttattt acaatatcag tatttctatt tgatatttca aacagtaatc
     3361 gttctaaatt acttgaaatt aatatatcca tagaaggaga agtagttaat ttcaatgttc
     3421 tatttttatc atatacacca gttttaaaga aatcataaag aacattatta tcatttgatg
     3481 cacatatcaa tttgtttatt ggaagaccca tttgtttggc ataatatcca gctagaatat
     3541 taccaaaatt accagttggg acaacaaaat ttattttatc atttaaattt atatcactat
     3601 tgctaactaa tttcatatat gaataaaaat agtatgcaac ttgtggaaga agtcttccta
     3661 tattaattga atttgcagaa gataatatat aattattatc caatagtttt tgattaaatt
     3721 ctttatcaga aaaaatcttc tttacacctg attgtgcatc atcaaaattt ccttttatac
     3781 caactacata tgtatttttt ccagtttgag ttttcatttg aagtttttgt actgggctta
     3841 caccatcttc tgggaagaat acaattatct ttattttatc tatatcttta aatccctcaa
     3901 gtgcagcttt acctgtatct ccagatgtag cagtaagaat cacaacatct ttctctaaat
     3961 tatctttctt tatagatgtt ttaagaagat gaggcattat agtaagtgcc atatctttaa
     4021 atgctaaagt tggaccgtga tatagttcta agaaataagt atcatttact ttatttaatg
     4081 gagctatact actacaatca aacttttcat catatgcatt ttctatacaa tttttaattt
     4141 catcttcagt aaaatcacat aaaaatttac taagtacaaa aaatgctatc tgagaatacg
     4201 ttaaatttac taaatttata agttcatttt ttacattggg aaattcactt ggaacataaa
     4261 gaccaccatc acttgatatt ccttttatta ttgcttgtga tgcagttact ttttcttcgg
     4321 tgcctctcgt acttgaataa tacatttttt ataatcctcc atatatttta aatttttaat
     4381 tagctaatta aattagatac agaatatttt aaaaactgaa tttaggtaat aaaaaaagac
     4441 cctcatccct aaaaaaggga cgagaatctg tttcccgcgg ttccacccaa attgatttta
     4501 aaaaatccac ttgatacttt aacgcaagta ataaaaacgt ctattcctac taaaatcatt
     4561 aagataactt cggatagaag ctcctaggta gttttcaatc cttctaatta gggaaacatt
     4621 tcagctagta gtttccttct ctggtaatcg attcagattt actcttccta atcaatgcct
     4681 ttcaatattc ttaatattat taattattct agtttataaa acagacaaag tcaagtatta
     4741 atataaaaat ttggaaaaaa tattttttaa attgtaaaaa aattgtaatc aaattgagta
     4801 ttagaaaaat atttttttaa cttttttaaa agtttaaaaa cctagatttt catagtgatg
     4861 aagctgtttt taatgaaagt aatcaaaaga taactattaa atatctgtaa tattacaatt
     4921 ttatactaca ctatattaca aaaaaagttt acaaaacttt aatttgaaaa atgtaattat
     4981 atggtatatc atataaatat aagtaaatag tgctaatatt taaactatta atcataattg
     5041 gttcaacaaa agtatatagt taataatgtt taatttgaag aaattgttag tgtgaaatta
     5101 catatatatt caaattaaat tcataaatat ttcacataat aatagtatac tgtaaacaaa
     5161 aggaggtatt aaatatggga atagtactta taacagcagc attagttttg gttatgtatg
     5221 gactttcaat tctatcatat atatcagaaa gcaatatatt agaagacact gtaaatgata
     5281 accaataagg ttttgaatta tataaataag tgagaactgt aaataaaagt taatttttat
     5341 ttgcagtttt tttatatata tgtaaaataa agttaattaa taaaaaataa tgttttgttt
     5401 atagtatata tggataataa tagtaaagta taagaatgat gagaataata tataagaaat
     5461 aataatataa agagaggtaa acgttatggt agatatcatt gtaattggtg ctggaccagc
     5521 aggtttaact tcagcaattt atgctatgag agcagggtta agcgttacag tatttgaaaa
     5581 aaatatttat ggaggtcaag ttgcaagtac ctctgaggta gaaaattatc cagcagttca
     5641 aaagatttct ggggtagaat tttctaataa tatatacaat caagcagttg ctcaaggtgt
     5701 tgatattcag tttgatgagg ttgaagagat aaatttagag ggaaaagtaa aggttgtaaa
     5761 aacttcttca ggtgaacata aagcaaaggc tgttattctt gcaaatggag ttgaaagacg
     5821 taaattaggt tgtgcagggg aacaagaatt tacaggtaga ggagtatctt actgtgcaac
     5881 atgtgatgga gcctttttta aagataaaga agttgctata gtaggtggag gaaatactgc
     5941 actagaagat gctttatttt tagctaataa ttgtacaaaa gtgtatttaa ttcacagaag
     6001 agatagtttt agaggtgaag aagtgttgga gaaatctgtg aaagcaagag agaatattga
     6061 gatactttac agtcatggtg tagaaaaaat agaaggagaa aagactgtat cgaaaataga
     6121 agtaaaaaac ttaaagactg aagaaaaaag aactatagat gttagtggta tttttattgc
     6181 tataggtctt aaacctaata ataaaatgtt tgaaaatgtt cttgatttgg atgaaggtgg
     6241 atatataatt tcagatgagt cttgtacaac atcagtagaa ggtgtatatg tagcaggaga
     6301 tagtagaact aagtttttac gtcaaataat aacagcagca tcagatggag ctattgcagc
     6361 cgttcaagca gctaattata taaatgttga ataatataaa tttcaaataa aaatagccat
     6421 ataaactgaa ataatttttc agtactatat ggctattttg ttaattagag gtaaatgtac
     6481 gttttattta ccttgtctag atttgttata tcttaatcta tcatgttcat ttaagaatct
     6541 ttttctaagt cttatagaat caggtgtaat ttcaactaat tcatcatctt ctataaactc
     6601 tagtgcttct tcaagagtaa atattctagg aggagataat tttatagcat catctgaacc
     6661 agaagcacgt acgttactca ttttcttgtt tttacaagga ttgactacca tatcatcttt
     6721 tctggaattc ataccaatta tcataccttc gtaaacttct acaccagggt ctacaaacat
     6781 tacagctcta tcacttaagg cattaagaga ataccccata gtaacaccag gtccttgtgc
     6841 tattaaaaca ccatttcctc ttgaaggtat ttctccttta aattcttcaa atttttcaaa
     6901 tgaacgaact aaagtacctt ctccacgagt agcgtttata aattcacttc gatatcctaa
     6961 aagaccacga gtaggtgcta agaactctat ttttacatag tctccttcta ttgacataga
     7021 ctccatcata ccttttctca tgtttaattc atttataata gtaccagagt aaacttcagg
     7081 acagtttaca acaactcttt ctattggttc cattagtttt ccatcttctt tatgcattaa
     7141 aacttcaggt ttagaaacac caacttcata accttcacgt ctcatatttt caagtagtat
     7201 agataagtga agctctccac gtccagaaac tttgtatcca tcagtagtat caagaggttc
     7261 aactttaaga ccaacattta cttcaagttc tttttcaagt ctatctttca aatgtctagt
     7321 tgtaacgaat tttccacttt ttccaacgaa tggagagtcg tttactaaga agttcataga
     7381 aagagtaggc tcttctattt ttatcatttc cataggtagt ggactatcta aatcgcatat
     7441 agtttcacct atagatatat caggaatacc agctataaca actatttctc cactagtagc
     7501 ttcatcaact tcaacttgtt ttaaaccttc atacacagaa agttttgata cttttccttt
     7561 agatacaaca gaatcttctc tacatatagc aacttgtgta ttgtttttaa gagtaccttt
     7621 gtaaattcta cctataccaa gtctacctac ataatcatca tatgcaagag ctgatatttg
     7681 gaactgtaga ggttcattat cgtaattagg ataagcttca acgtgattta caatagtttt
     7741 aaatagagga gaaaggtctt cactatcatc atccatttca agtttagcaa taccttgttt
     7801 agcaatacca tatattatag gaaattcgca ttgctcatct gttgcattta agtctacaaa
     7861 taaatcgaaa acttcattaa ctacttcttc cgctctctgg tcttttttat caatcttgtt
     7921 tataaataat attggtttta aaccgaactc taaagatttt tgtaaaacga atctagtttg
     7981 aggcattggg ccttcactag catcaactaa tagaataact gtgtcaacag ttttcataac
     8041 acgttcaact tcagaagaaa agtcactgtg tcctggagta tcaactatgt ttattttgta
     8101 atcttcatag tttattgcac agttttttga atatatagtt atgcctcttt ctttttcaag
     8161 gtcgttactg tccataacgc agtctttaac tacttcgttc tttctaaaaa caccgctttg
     8221 agaaaggaaa gcatcaacca atgttgattt tccagcgtca acgtgggcta taactgcgat
     8281 atttataatt ttatgttttt gtgacatttt tgttgctccc ttcgattaaa ataacttatt
     8341 aattatagca caatatatta ttaggtacaa ggaggaatat attaaattgt attaatatag
     8401 tttaaattgg ataaaactgt tagttttgca tctgaaaatt attctgttta aaaaaatttt
     8461 ttagacaaat aataacttta atagataaaa agattaataa aagagtaaag gatgatatat
     8521 gttggaagta accaaaataa taattaaata ttttgcaagt gaaagtgata cttttgtaaa
     8581 agaagaagtt tgtaattttg aagaacttct taagtggtct cataaattta ttgaaggtaa
     8641 aaagatagaa gatgttttgt atataaagtc taaagaaaac tataaagtaa atgtgattaa
     8701 gtctttagaa attaaggaat tggaaatagt gagaaaacaa cagataccac cgtatatgta
     8761 gtattaataa tatataaaat taatatgtaa tactcgcaat aggatataaa tggtatatat
     8821 ggtaataatt tatattaaaa aaacttttat aaagtttata tttgggtata aaagtattaa
     8881 ttagacatta attaggagga acttaaatat gataagagta tgtccaaact gttcaaatgt
     8941 ggatgtaaat aagctaaaaa ctttggttgg agaagaaaat gtaaagactg gttgtatagg
     9001 tcagtgtaga gcatttaaaa aagaagctgt tggaataata gacaatgagc ttgaaataaa
     9061 ggaaacagaa gcagagtttt ttgaagcatg taaagaataa attttaaata aaatataata
     9121 aatagagcat ctaaattgta taagcattaa acaatttaga tgctctattt ttatatatac
     9181 atttaattat gagtatactt ttctattaaa tacaaataaa tataactaat acatttattt
     9241 attaaaaaaa tattgattta atatacctat agggggtata ataaaaatat agatttctaa
     9301 ataagtaatt tatcaatatt acatatcaaa ataaaacgtt acaaattagc tgaaaatata
     9361 ttgaaagaaa ggaagatttt attatgagtt caaataaagt ggtagaaagt tataaaatta
     9421 caggtatgac atgtgcagct tgtgcaaagg cagtagagag agtaactaag aagatggatg
     9481 gagtatatga ccaaagtgta aatatagcta ctgaaaaact taaaatagaa tatgataatt
     9541 ctaaggttaa ttttgatgat ataaagcagg ttgtagaaaa agcaggatat ggaattataa
     9601 aggaagaaag taataaaaaa attgatatga aaattgatgg aatgacatgt gcagcttgtg
     9661 caaaagctgt agagagagtg gtaaaaaaat tggatggagt agaaagtatt agtgtgaata
     9721 ttgcaacaga taaagctaat atagactatg acccatcaaa agtaaaatta tctcaaataa
     9781 aagcagctat tgaaaaagct ggatataaac caatagaaga ggtaagaaat aaggttgatg
     9841 tggatgaaga taaattgaga aaagaaagag aaatgaagtc tttatttgtt aaatttatag
     9901 ttgcaatagt atttgcagta cctctgtttt atatagcaat gggacctatg ataataaagc
     9961 ctatagggcc atggcctttg ccagaaatta taaatcctat gacaaatact tttaattatg
    10021 cattaattca gttaatttta gtaataccag taatgatagc tggttataaa ttttatataa
    10081 atgggtttaa atcacttttt tctttaagcc ctaatatgga ttctttagtt gcaataggaa
    10141 cattagccgc atttttatat agtctatata cgacactaca aatagccaat ggacaaattc
    10201 aaggtatgca ccatcatcag ttgtattatg aaagtgcagg aataataata gcacttatct
    10261 tacttggtaa atatttagaa tcaaaatcaa aaggaaaaac atcagaagca attaaaaagc
    10321 ttatggggct acaacctaaa actgcaatag tgttagttga tggtaaagaa gttgaaactc
    10381 ctatagaaga agtagagata ggagatatac tattggtaaa accaggaact aaaatacccg
    10441 tggatggagt tgtaatagaa ggatatacat cagttgatga atcaatgctt acaggtgaaa
    10501 gtataccagt agaaaagaat gttggaagta aggtgacagg agctagtatt aataaaaatg
    10561 gtgttattaa gtttaaggct gaaaaaattg gtggagatac tgctcttgcc caaataataa
    10621 agttagttga agatgcacaa ggtacaaaag cacctattgc taaacttgcc gatactgtat
    10681 ctggatattt tgtaccaata gtaattgcaa tagctgtagt tgcatcatta ttgtggtttt
    10741 taataggagg aaaagatata gtatttgtac ttactatatt tatatcagta cttgtaattg
    10801 cttgtccttg tgcattagga ttagcaacac caactgctat aatggttggg actggaaagg
    10861 gcgcagaaaa tggaatcttg ataaaaggtg gagaggcact agaatctgca cataaagtaa
    10921 atacagtgat atttgataag acaggtacta taacagaagg aaagccaaaa gtaacagata
    10981 tagtattaaa taataatgta aaagaagaat atcttatcaa gattgcttca agtgcagaaa
    11041 aaggttcaga acatcctctt ggagaagcaa tagttaagta tggagaagag aagaatataa
    11101 aatttgaaaa agttgataat tttaaagcta taccaggtgc tggtatacaa gttactatta
    11161 atgatgaaag tatattactt ggaaatagaa agcttatgaa tgacaataat ataaagcttg
    11221 gggatttaga agaaaaatca aatatattgg caagccaagg taaaacacct atgtatatag
    11281 ctgtagatgg aaatttatca ggtataatag cagttgcaga tgtagtcaaa gaaagtagta
    11341 aaaaagcaat agaaatactt catgatatgg gaataaaagt tgcaatggta actggagata
    11401 atgcgaaaac tgcaaatgct atagcaaatc aagtaggtat agatatggtt ttggcagagg
    11461 ttttaccaga agataagtca aaagaagttg aaaaactaca aaatcaagga aagtttgttg
    11521 caatggtagg ggatggaata aatgatgccc cagctcttgc aaaagcagat atagggatag
    11581 caataggaag tggaacggat gtagccattg aatctgcgga tatagtactt atgaagagtg
    11641 atttaattga tgttccaaca gcaattaaac ttagtcatga aactataaaa aatataaaac
    11701 aaaacctatt ttgggcattt ggatataata caattggtat acctgtagca gcaggaatat
    11761 tatatgtatt tggaggacct ttattaaacc caatgattgc agcagcagca atgagcttaa
    11821 gttcagtttc agtcgtatca aatgcactta gacttaaaaa cttcaaggca tataagagag
    11881 attagattaa taaattaaat aaaatgtatc caaaagagct atctttaaat taaaaggatt
    11941 aatttacaaa ttaagatagc tcttttttca tgtaaaattc acaacaataa gataaaatat
    12001 aagtaatata cgaaatttta aataaaatag aaaggattga tagaatgaat tcgtcaatac
    12061 ttgtaattga agatgattca aatatacaag agttaatatc agaattttta agtgcagaag
    12121 gttatcaagt tgatacagca aatgatgggt tggaaggtat acaaaagttt aagcaaggaa
    12181 gttatgattt agtaatatta gatataatga tgccaaactt agatggttat ggagtttgta
    12241 aaatgataag aaagtcatca agcgttccaa ttatattttt aactgcttta aatgatgaag
    12301 gagaccaact taaagggttt gacttagaat gtgatgatta tataacaaaa ccattttcat
    12361 ttaatcttct tataaaaaga gtagaagcaa ttttaaggcg aagtaacaag actataaatg
    12421 acaaatttat agtttttgaa aaattaaaat tagatttaaa cacatatatt gcagagatag
    12481 atggagaacc tatagaactt acactaaaag agtttaatat attaaaagct ttgatagaaa
    12541 agtatccaca agttataact agggaaggtc ttttggatag tatatggggt tatgactatt
    12601 atggagatac taggattgtt gatgcacata taaaaaatat aagaaaaaaa atatcattac
    12661 catatattaa aactgtaaaa ggaataggat atacacttga gaaggatatt tgataagtgg
    12721 gaaaagttaa gtataaagta taagttattt tcaataacga catcattatt aatagcatta
    12781 gctttaataa tatatctaat tttatatttt ttattgccat catattatca tgagtataaa
    12841 attgagtctc ttcaagaaag tttgaaatct ttagtagata gttcaattca tttcgataca
    12901 tatactttag aagaaagact ttattatatg gctaaagacc aaaatttggc tattttatta
    12961 aaagataacc aaggtaaaat tgtatatggt aagaatgaag tagttatttt aagatatagt
    13021 aagtacatga ttaatagtct tgaggatgag tatagaactt ctatacctat atatactaaa
    13081 gatgcaaaag atggtccata tactttagaa ctcgttatgc cattacaacc aattgacgaa
    13141 gcaaacgaag ttataagaaa actaatgcca tacatcatat caatagcaat attgatagcg
    13201 attattggag catatatata ttcaattgta ataacaaagc ctcttataaa cataatagag
    13261 agtgagagag aacaggaata tagaaggaag gattttgttg ctacaatatc acatgaacta
    13321 aaaactccaa taaccataat aagtggtcaa attgaaggta tgatttatag tgtaggtaag
    13381 tataaggata gggatactta tctgaaaaaa tcgtatgagt gtacacaaga attaaaagat
    13441 ttagtaaatg aaatgataga ggtttcaaaa tcagaaatac ttgaaaaaga tttgaaattg
    13501 gtatcaataa atataagtga attattaaat agacttgtaa agagacaagt atttttaata
    13561 gaagaaaaac atatgaaaac tattttaaaa atagaagaaa atttggaaat taaagcagac
    13621 caagagagaa taactaaggc aatcaataat ataataaata atgccataaa atattctcct
    13681 gaagaatcag agcttataat tagactctat gataaaaata aacgaataag taaaaaaact
    13741 tctaatcaaa gagtggtttt agaaattgaa aatactggtg taactatcga gaaacgttat
    13801 ttagaagaaa tatttaatcc attttataga atagaaaaat ctcgttcaag aaaaactgga
    13861 ggaagtggac ttggacttta tatagtcagc caaattttta aaagtcatgg gtttgattat
    13921 agcataaaaa acaaagaaaa ctctgtagta ttcacagtag aatttaaaaa ttaattactt
    13981 attataaaga atatataact ttcaataata gttcatttta aaatcacaaa aaattcataa
    14041 tgaaactgta ttatataaat ataaagaagc ttaaaaccat aaaatacacc aactataatg
    14101 atgagatttc cttacatcct ccatataagt catcattata gtaacccaac aattaagtta
    14161 ctatgaattt aaaaaataac accgagcata taatctatgc ttagtgttat ttttattttg
    14221 aaataaaaaa tatttaattt attgaaatgt atagatttag atagtttaaa ctatatttaa
    14281 gacttaaaaa ttataattga tattacaaat aaatatatgt actttgggaa aaataaatat
    14341 aggaagtatt atatttacag aggaggtagt tttgatggat agattaaaaa gagatatgaa
    14401 aaatatgaaa gaagatttaa aagaaggtgt taaaaagaca gcttatgtta tgaaaaatac
    14461 agctgaagat ttgaaagatg atgcacaagg tgctatggta aacttagaaa tgaaaaaaga
    14521 tgagatgatg gaaagatatg aacaaaagaa attttctaaa gaattagaga agcaaatgaa
    14581 gaatgaaatg aaataagctt ttatttatga ataacaaatt attgtattca tataaaaagt
    14641 agccagtaat attaaatagt ttatttaagg aaatagactt tctaaaaata actgtattag
    14701 atttagaaag tctatttcaa attagtttta taaatagaca taaggataaa gtagatgtta
    14761 tagaactaga ttttaaa
//
LOCUS       DAMJYC010000097        14459 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJYC010000097 DAMJYC010000000
VERSION     DAMJYC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.101"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            102..1730
                     /gene="aspD"
                     /locus_tag="PC472_003138"
     CDS             102..1730
                     /gene="aspD"
                     /locus_tag="PC472_003138"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2728447.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEGLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1851..2846
                     /locus_tag="PC472_003139"
     CDS             1851..2846
                     /locus_tag="PC472_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2728448.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3035..3418)
                     /locus_tag="PC472_003140"
                     /pseudo
     CDS             complement(3035..3418)
                     /locus_tag="PC472_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3644..3741
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3883..4378
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3883..3911
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4664..4822)
                     /locus_tag="PC472_003141"
     CDS             complement(4664..4822)
                     /locus_tag="PC472_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728449.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5181..6551)
                     /locus_tag="PC472_003142"
     CDS             complement(5181..6551)
                     /locus_tag="PC472_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2728450.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6834..6932
                     /locus_tag="PC472_003143"
                     /pseudo
     CDS             6834..6932
                     /locus_tag="PC472_003143"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6951..7372
                     /locus_tag="PC472_003144"
                     /pseudo
     CDS             6951..7372
                     /locus_tag="PC472_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7603..7875
                     /locus_tag="PC472_003145"
     CDS             7603..7875
                     /locus_tag="PC472_003145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2728451.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8238..8318)
                     /locus_tag="PC472_003146"
                     /pseudo
     CDS             complement(8238..8318)
                     /locus_tag="PC472_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8358..9026)
                     /locus_tag="PC472_003147"
     CDS             complement(8358..9026)
                     /locus_tag="PC472_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2728452.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9862..12282)
                     /locus_tag="PC472_003148"
     CDS             complement(9862..12282)
                     /locus_tag="PC472_003148"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2728453.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVETFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12636..12986)
                     /gene="rsfS"
                     /locus_tag="PC472_003149"
     CDS             complement(12636..12986)
                     /gene="rsfS"
                     /locus_tag="PC472_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2728454.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13103..13675)
                     /gene="yqeK"
                     /locus_tag="PC472_003150"
     CDS             complement(13103..13675)
                     /gene="yqeK"
                     /locus_tag="PC472_003150"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2728455.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13676..14365)
                     /locus_tag="PC472_003151"
     CDS             complement(13676..14365)
                     /locus_tag="PC472_003151"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2728456.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4880 a         2350 c         1842 g         5387 t
ORIGIN      
        1 actttttcta taaacctatt ataattaaag tataatcatt tttgcaactt gtttatttta
       61 ttataaaaat gtgaaactta ttacataagg aggaatttaa catgaaaaat catacttata
      121 aagaaatcaa aaacatatat ggaaaaataa gcccttttga atttaaggac aaattaatag
      181 atattgcaaa atacactgca aaagaaaata acagagaatt acttgatgca ggtcgtggaa
      241 atccaaactg gacttgttca actgctagag aagccttttt tacttttggt catttcgcta
      301 ttactgaaac tcgttctaat tgggatttag gtcatttggc tggaatgcct caaaaaaagg
      361 gtattaaaga aagatttttt aaatttataa atgaaaatat agatatgcct ggagcatatt
      421 tagctagaga tattataaac tttggtataa atgaacttgg atttgatgga gatgaatttg
      481 ttcatgaact agctgatgga attattggtg ataactatcc acttccagat agaatgttac
      541 ctcatatgga aaaaatagta cacgattacc tagttcaaga aatgaaatat gatatatcag
      601 gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc tacagctgct atgtgctata
      661 tatttgattc attaatggca aatgaacttc taaaaaaagg agatacaata gccttaatga
      721 ctccaatatt tacaccttat cttgaaatac ctaaccttcc tcgttacgac tttaaagtgg
      781 taaatataaa tgctaatgaa gttgatgaaa aaggtgctca cacatggcaa tatacaaaaa
      841 aagaattaga gaaacttcgt gacaaatcta ttaaagcttt attcgttgtt aatcctaata
      901 atcctgcttc tatagctatg gatgaaacat cttgtaataa tctaatagat gtaattgaaa
      961 attataataa agatttaatg ataatatcag atgatgtata tggaactttt gtagaagatt
     1021 tttcatcctt aatgtcaaaa ctaccttaca acactgttgg agtatattct tattcaaaat
     1081 attttggagt tacaggatgg agacttggta catttgccct tcataagaaa aatgtttttg
     1141 ataagaaaat aaatgattta actggagaat taaaaaaatc agtagataag cgttacagtg
     1201 atatgagtct taacccttct agcctttcat ttatggaaag agttgttgct gatagcagac
     1261 ttgttgctct caaccatact gctggtcttt ctactccaca gcaagtacaa atggcgtttt
     1321 tctctgcttt tgcattgatt gacaaagtag atgcctacaa aaaactcaat atgagtatct
     1381 gccatagacg tcaaaaacta ttatttgaag ctttagagct tccaattaat gaaaacaaaa
     1441 acaatgctgc atattacact caatttgata ttgaagaatg ggctaaatta aactacggag
     1501 aaggcttatt taaatttata agtaaaaatg catctccagt agatgttctt tataaattag
     1561 ctaatgacta ttcagtagtt ttacttagtg gaaatggatt ctatggtcct gaatggtcaa
     1621 ttagaatatc tctagcaaat ctatatgatg aagcttatac taagatagga aaagccataa
     1681 gagagatatt aaatggttat atcgttgaat ggcaaaaaat taagaaataa ttatattaca
     1741 ctgaaatatc taaattatat gaataaaaga gtttatgtag accttaacta ctttttttga
     1801 tataatccta tcggaattat aaattttatt tatgaaaggc agagaatagt atgagtatta
     1861 agaaaaaagt tgccatagtt tttactggtg gaactatatc tatgacagta gatgataaag
     1921 tcggagcagc tataccaact ttatcaggtg aacaaatact atcaatggtt accaacattg
     1981 ataaagttgc agatgttgaa gtttttaatt ttgatgaaat tccaggacct cacataacac
     2041 cttatagaat gatggaattg aaaaactatg taaatgattt attagcaaga aaagatatta
     2101 caggtgttgt aataacacat ggtacagata gtttagagga aacagcttat tttctagacc
     2161 ttacaattga caacatcaaa cctgtaatag tcacaggtgc tatgagaagt agttctgaac
     2221 ttggatatga tggttcaagt aatttatctg catcagtttg tactgcagta tccaaagatg
     2281 ctatgggaaa aggagtcctt gtagttttaa acaatgaagt attattagct tctgaagcta
     2341 ctaaaacaaa tactctttct ctaaatacat ttaaatctct aactagtggc ccattgggaa
     2401 taattgactg caacgaactt gtccttacaa gagatattgt aaatagaaca attatagata
     2461 ctgataaggt agaatctaaa gttgccttat ttaaagcata tgtaggagaa aatgctgact
     2521 ttataagatt tgctgttgat agtggttata aaggtatagt tattgaggca atgggcagag
     2581 gtaatattcc tcctcaaatg ctttctggag ttgaatatgc tagagaaaaa ggacttcctg
     2641 tagttatagt ttctagatgt cattcaggta gagtattcga tagttatggt tactttggtt
     2701 ctggtagaga tttaaaaaat cttggttgta tatttggtgg ggacttacca ggtcaaaaag
     2761 ctagaataaa acttatctta gcacttggaa aaactgataa ccttgatgaa atcaaagatt
     2821 tctttgaaaa aggaatctat tgttaattct aataacttct atttaattat ttattgatat
     2881 ttcaacattt ttaaatcaaa atctaatact tatgtcagtt aaatgtcagc aaaatatttt
     2941 atccacaatt tttacacaaa ttttccacaa cttatcccca atattatcca caagaaaaaa
     3001 ggtagcactt atattagtag ctacctttta tttattaatc ttcatctttc cagcttttta
     3061 tccaatttcc tatttctatt attttttctt cagttatttc ttctccatca tgtaattttg
     3121 aaattagttc tgataatgga ttgttatgta gacctccaaa aattcttttt ccctctattt
     3181 tgagatattc tttctctttt attaatactc tatagtgtac atgtttccca attctctgcg
     3241 atatgaaaaa acctttttta gttaatctag ctaaaagtgt aattgtagtt gtatgcttcc
     3301 aagcatatgt tttttctatt taatctgcaa catcttttga tattacagtt ttataaccag
     3361 tattccatat gtatttcata atttttaatt ctgcatctgg taatttttta ttcaacataa
     3421 tatattcctt ctttcaattt atgtcacttc ctcaaactta tctagcttaa attaataata
     3481 ggagcttttt atcacaaaaa ctacaggtaa atttttctat atattctacg tatttagtct
     3541 gtttttaagt gtgaaattac gttcatacct ataaatttat tcaattttca aacaatcttg
     3601 ttgaataata actaattcta ttgtattatt tagttataga ataaaactaa atcggcaaaa
     3661 ctagagaaat ctagtgacgc aaagctatag ggactaagac ttatataaat atcttatgag
     3721 ttatgttagc cagttgccaa agagatattg ttctttttgt ttttatgaaa gttttttagg
     3781 gggataataa tttatatgtt tagagataaa atggataaat gtacacatat attgactgct
     3841 tatattagca gttcgtatga ttattgtaat tttttggata caatttacat tccatatagt
     3901 taatataaaa cttgtgttta ttgtgatagt gccgaacagg tgttaatacg atttacattc
     3961 catatagtta atataaagct ctaatttaat agctttattt ttagcaaaat acccaaattt
     4021 acattccata tagttaatat aaaaccggtt ccctccgaag ttcatagaga ttcttatcag
     4081 catgatttac attccatata gttaatataa aacaaatgct gaaagataat tcggttgggg
     4141 atttaatgaa atttacattc catatagtta atgtaaaact aaatagagca gatgaggctt
     4201 ttcaagaagc cgatagattt acattccata tagttaatat aaaactctga tgttgtgaga
     4261 tttgaaaaaa acggtaaatt gaatttacat tccatatagt taatataaaa cgctgataaa
     4321 gttattaaaa gtaaaattgc tgaaatacta tttacattcc atatagttaa tataaaaccc
     4381 caaaataaat tgagtatttc caatgcctac acatatacaa atctctcaaa tttgcagtga
     4441 accatgagta gtgcaattga taacacttat cacaagtcct caatgcctta tatttcaact
     4501 gttaagcctt aatttcttca aaaaatcgaa cactgcaaaa tttctatgac tttattatat
     4561 cataaaaata ttatttttga atatctgtac caatttgtga tataataaaa gcaagaagaa
     4621 ctacaatcta ttttgctgta gagtggagtt cataatttag aaattactta gttttatgga
     4681 atctgatttt taaatcaaat tcccaaccac tcttagttgc cgctttgagt ggttttttac
     4741 gctttctaaa tagtttgcta gctatataaa ctattaagct agcagataga cttgctagta
     4801 tgccttgtaa aaaattatcc atgtatattc acctcccttc ttagcgtcgg gaggataatt
     4861 ttttgataca tgaactccac tctataaatt gtagattaca tcttcttgct aaaaatatta
     4921 taacatataa ttcttacata ttttacctat ttattacttt ttacattata ataatattct
     4981 tttaatcaaa aaaacaccta ctattaaagt aaatgttttt aagtattttt aattttaaaa
     5041 tccatatagt tcatatgaaa ctaaatataa taactcaaca tatgagcaat atcgagacat
     5101 ttacatacca catagttaat ataaaacgaa aataaaaaaa ttaaacttat aaaaccctta
     5161 ttaatttaca tacccactag ttatttatat tcaaaaaaat cttctccatc tgggttataa
     5221 aataatattc tacttgccaa tccttgttca tatttataat tttctttatc acctacataa
     5281 gaaaatgtta atgtcatgct tcttgatgga tctaatgaaa ttatatctaa ataaatagta
     5341 catttttctc ctggctttaa tacaccgaaa ctattaaaat ccatttttat tttagcatat
     5401 tgccccatat ataatgttga attagcttct ccataaactt ttaattttgt tacatctaaa
     5461 ttttgtgtac ttgtattttc tatatttata ggaactgttg ggtctttata tttttcagta
     5521 taatattcaa ggttatcatc ataacattca agataacttc ctttatcttg ccaaattatg
     5581 tgctttgaat caaaatcaat ctttaatcca catattatat ttttatttga agacggaaga
     5641 ctaatattat tgatggtatt ttcacactct agcataattt tatcacttag atttcctatc
     5701 ccagtcactt ttgcagcatt tttaagtata tctttattac ttccaatatc aactagtaca
     5761 ataggattat ttttagccat tggtgaagca actaaagcat aaactaaatc agtaccacta
     5821 gctatataaa attcttttgc gccattatag aacttattta ctattttttt attagtatca
     5881 tatctatcta ctccacctaa tctagttgaa ttagtatcat taactagttt gtcactcatt
     5941 gatgaagtac cacctattac ataactttct acaccagttg tattaaatgg aacacttttt
     6001 ccatcagtta atactatagc tgctttatct ctaactgcta caggtgcagc actcattgca
     6061 tcaggctctc ctttaaaagc attagtcaat ataactttat ttactttgtt aattgagttt
     6121 atttcttttg caacattata gcttgtctta attctatcac taccttgaag tcttttagcc
     6181 tctattcctt tatcttttag aagagtttct gttgctttat caatagaact ttctccacct
     6241 attatgtata cctttttagc tttctctact cttttaagag ttgcattagg tatgttattt
     6301 ttcttagtta aaaaaattgg tgcatttgtt gctcctgcaa gtccactcgc acttaatcca
     6361 tcagccattg tgctgtctgc attaattaat atagctgtag tataattttg ttcatcagct
     6421 ataagagcag ccgtttcata tttatctttt ccttgaatag tataaattgt atcaagtgca
     6481 ttagctgata taggaccagc agcaaacatt gacacggcta accctagtga taataatttc
     6541 tttaatttca caatatctcc cccttttata tatgaaatac tatatatctt acataattct
     6601 acattaaatt ttcttttcct cctttaatga tatttatctt cctgttaatt taagtgtata
     6661 caaatacgtg ttataatgta actgtaaaga attaaaaaga taatgacata attagagcag
     6721 tccaactgtt cttttataaa actaacttat aaagggagcg tacatatatg aatatcaaat
     6781 cagcttttat aagaaaaaga tgggaaaaat tctatgtata tgtggaataa gaaactggca
     6841 aaaagaaaca aaaaagttat ggaagctata aaaagaaaaa ggatgctgaa aaacatttaa
     6901 ttgaaataaa gtctactata aataataata agtttgttgc tccaaaaggt atgacatttg
     6961 tagaaagatg ttataaatat atagacatga ataaaaataa ttggtctcca tatactgtta
     7021 caaatacaaa atcttgggtt aaaaattaca tagaaccttt tttaagaatc taaaactcac
     7081 agaagtaaat cctagttcag tacaagcttt tgtaaattat aactttaata attctacctt
     7141 tactagtgct aaagtaaggt atagctttct aagttctgta ataagggaag cgtatagatt
     7201 aagagaaata caagaaaatt catgtgattt tataaaactt ccttctaaag aaaaatattc
     7261 tacaaatgaa atatacaata gagaagaagt attattgcta atagaaaagt tacttggtaa
     7321 taatatagaa atacctattc ttttaatgtt acttctaggt ttaagaatgt gagaagctac
     7381 tgggcttagg tggtcagatg ttgacttaga taacaatctg ataaatgtta atcaaattct
     7441 tgtctattct aatggcaaaa tatcttttaa agagccaaaa acaccaaaat caaaaagagc
     7501 tttatcagct ccaaaagaat taattgaaaa attgaaaatt gaaaaactaa gacaaagcaa
     7561 attaaaattg taaggtatcc tggaaaacag aaataactta gtttgtctaa acaccaattt
     7621 aaatccgttt ctaccttctt cactaagaaa aacgtttcac aattttacaa aaaaaataat
     7681 ttaaaaaaga ttagaataca tgatttaaga tatacaagtg ccactctgtt gcttttaggg
     7741 ggaactaata tgaaagttgt ttctgaaaga ttgggtcata cagatataaa aataactatg
     7801 aatagatatt ctcatgtact taaagaaatg gacaaagaag cttccgacaa tctaagcgaa
     7861 cttctattta aatagtaaaa actgactgtc agttaaatgt cagttaaacc taaaaaaata
     7921 ggttttgtca gttgattatc agtcaattat tttataatag tttcacttaa aaacgtctat
     7981 atctacacat tttactattt gtttatgctt attaatatct atctgttatt attaattcta
     8041 atagcttcta tttaattatt tagttcagtt tacaggaaac tacacacaaa ccataaaata
     8101 ggatgttgac aatataacag atagtatgct cctgtaaaag gtaacatact atctgttatg
     8161 ttgtctggat ttccaaatgg ttaccctttg gcacacgact ttgctatctc cttggtaaac
     8221 taaaagtttg taatctaaaa tcctaatcca tctctataaa atttaattga cttctccata
     8281 ctttttacac ctaaacatat aaaagtaatt ttattcactt aatgacctcc ataattccca
     8341 aattagctat ttggaactta actaaaaaat cctaatataa gggcaactat tataatggca
     8401 atcggaatac atactgtgta atcctgttcc atagagtcat catctgaatg tagttttaca
     8461 attcgcaatt tagcaatcag tttgaagcaa aaatagccta ttattgttcc aattacgttc
     8521 gtgagtaagt cgtctatatc ggttgctctg tgtaacgtaa aaagctgaat agtttcgata
     8581 aaaagagata atccaaaacc aatcaagaat gtatttctca aacgttcaaa ggttttgctt
     8641 ataagcggac ataaaaaacc taatgggaca aaaagtaaaa tattcaaaat aaaactcaaa
     8701 ctaaatccat cactaaaagg aataagattt atgtttggat taaagaacgt ttcccctaac
     8761 tgtgacagtc ttatatattc accgaatgta ggtataccaa caatatgtga tagtgttaaa
     8821 cataagtagt aatacattgc cagtgataaa atagatgttt ttacctttgg aatatcacta
     8881 tttttcttga atattacaac taagaaaata ccaatcaaga taattcccaa caagaaaata
     8941 ggattaatta acaataaatc tttgatagct tcaaacagac tgagttgaat ttctacacct
     9001 gattgattat ttgttacagt ctccatttgt acatacctcc tcataacatt tcaattattt
     9061 ctaaaaaaca aaatagaaga taaatcttaa aatcatccta tcaaaatttt taaatttgcc
     9121 tttatcagtt tataccacca gttaattcct gtcttacttc tgtatggtat tcttgggatt
     9181 ctttcatcaa attagacaaa aaatggttac catatcaata aactggttac gctccatatc
     9241 tcccgaagca accatcccct gagaataaat ccgtatcagg aatctccaca tctactgcac
     9301 ctatcacaag ttatttgata acatcctcag acaatcccaa cttggatata tcgacaacac
     9361 ccattagaat accactgctg accatacttg ttttcctgct gataatttta atcaactggg
     9421 aagttgaaac gctatctttt ttacaagttc ctttttagaa acatggtagc tattatatga
     9481 atctgaattt cgttgtctgg gtgtttcata taaagcatag atatgcacct tttttgcctg
     9541 ttttcattat atagaaaaaa atttcaaaat acaataatta tatttttttc gtttaagctt
     9601 agagcagttc cttgatgaac aacactttgg aaactgaatg atttacatac cacttaatac
     9661 attaactcga tttaacatta attataataa tatttaaaac aacaactagg gtatataaga
     9721 atgaactttt tagttcttta atcatttttt tgtataaaat caaatctatt tttataattt
     9781 caacaatgaa aaaaggctta tctctatttt gagacagcct catttatttt ataaatttat
     9841 atttttgttc atatttttga gttatttaac aactatattt actaattttt taggtacagc
     9901 tacaacttta actatagttt taccttcaac taatcctttt attttttcat cttctaaagc
     9961 taccttttcc atctcatctt tagatatatt tgagttaaca gttaattttc ctctaacttt
    10021 tccattaact tgtacaacaa cttctatttc atcttttact aaagcttttt cgtcatattt
    10081 aggccagtct atatcaaata cacttccttc tttacctatc atagtccata gttcttcacc
    10141 taaatgaggg gcaaatggtg caattatagt tactatagtt tgtacacctt cttttataac
    10201 tgcttcgttt atattatcta actctttata tttatacata tcatttatta attccataag
    10261 agctgatata gcagtattaa atccgaattt ttcacttaaa tcagcagtaa cttttttaag
    10321 tgtagagtgt atagtatatc tcatatcctt atcttgggaa tttaattctc caaactctac
    10381 atccttttta accacatctg ctaactcgtc aaccaatcta taaaccctat ttaagaatct
    10441 aaagcatcca tcaacacctt gctctgacca gtccaaatct ctttctggtg gtgctgcaaa
    10501 taatacgaaa agtcttgcag tatctgctcc atactcatct attatatcca taggagatac
    10561 tgtattccct ttagacttac tcattttaga accatccata agtaccattc cttgagtaag
    10621 taagttttta aatggctcat tgaagtctac cattcccata ctcttaaatg cctttacaaa
    10681 ccatcttgca taaagtaaat gcattatagc atgttctact ccacctatat attggtctac
    10741 tggatgccat ctattaacta attccttact aaatggttca ttttcattat tgctatccac
    10801 atatcttaag aaataccaag aagaatcaac gaatgtatcc atagtatcaa cttcccttct
    10861 agctggtttt ccacagtgag gacaagttgt tgacataaat tgctttgaag tcgtaagtgg
    10921 agattcccct tttccagtaa attcaacatc tgttggtaaa agaactggta aatcttcctt
    10981 tttaactgga actattccac attcatcaca ataaaccaca ggtataggac aaccccagta
    11041 tctttgtctt gaaactaacc agtctctaag tctataatta attgttctct ttcctctacc
    11101 ttctttttct aacttatcga ttattccttc aaatccttct gaagcatcta atccattaaa
    11161 ttcttctgaa tttatcattt tgttatcttc tgttattaca tcaattatgt ctaaattata
    11221 tttttcagca aaatctctat ctctttcatc atgtgctgga actgccatta tagcacccgt
    11281 tccatactca actaatacat agttagctat ccatagagga actttcttgc catttaaagg
    11341 atttataaca tatctaccta taaacattcc ttctttttct acatcagctg ctgttctttc
    11401 tatttctgtc atagtatgca ttttttgaac aaaagtttca acatctgctt catattcagt
    11461 ccctgcaacc aattctttaa ctaattcatg ctctggagca agtaccatat aagtaactcc
    11521 ataagttgtg tctggtctag ttgtaaatac tgtcatagat ttatcagttc catctatatc
    11581 aaaaactaaa tctgctcctg tacttttacc tatccaattt ttttgcattg ttttaacttt
    11641 ttctggccat ccatctaatg tatctaagtc attaagtaat tcttctgcaa attcagtagt
    11701 cttaaaatac cattgttcta aatctttctt taatactgtt gctttacatc tctcacatgc
    11761 tccttgaaca acttgctcat ttgcaagaac agtctcacaa gaaggacacc agttaacata
    11821 tgattttttc ttatatgcaa gcccatgctc taaaaacttc aaaaatattt cttgtgtaaa
    11881 tctatagtat tctggagttg aagttgcaac ttctttatcc caatcataac taatccctaa
    11941 taaatttaat tgctctttca tttcttctat attttccatt gtccatttat gcggatgtat
    12001 tccatgtttt atagctgcat tttcagctgg taatccaaaa gaatcccatc ccattggatg
    12061 aagtacatta tatccttcca tttttttgaa tcttgcgaca acatcaccta tagaataatt
    12121 tctaacatgt cccatgtgta tttttccaga tggataaggg aacatttcta atgtgtaata
    12181 attaggtttt tcagtttgtg cagtatccat tttatactga ttattatctt tccagatttt
    12241 ttgccatttc gattctactt ctttaaaatt atatacgctc atattttgcc tcctattttt
    12301 aattgtgtat aactacctta tttagtatta tttacttata atttccaaat aattctttga
    12361 aaataaaaaa tcctcgtcac taaacttacg tttagggacg aggataaatc ttcgcggtac
    12421 caccctaatt agcctactac atatatagac tctcttagat agcttttaat ccagccatgg
    12481 agaatagatt ttaatctagt aagttcaaag ttatataata ttagttctca ccttccacta
    12541 actctctgaa aaaatataat cttttactgc tctagtctat tcaatttaat ataatttttg
    12601 aaattttcta attataataa catatattat catgtttatg ctaatgtgtc aatatctata
    12661 taaggtgcat ctttccataa tttttctaaa ttataaaacc ctctattttc ctcattaaat
    12721 acatgaacca ttatgtctcc ataatcaagg agtacccaag tttgagttcc ttgtccttct
    12781 ttacctcttg gctctaatcc tagttttgct aattcatctt ccacattgtc agcaatagct
    12841 tttacctgtc tctgagaaga tgctgtagta ataataaaat aatcacacaa actagaaact
    12901 tttcctatat ttattataac tgtatcttga cccagtttat cttctatggc atcatatgct
    12961 atttttgtca tttgttccac tgtcatataa aaattcctca ctttcctaat taaaatacac
    13021 ttagttattg taacatatta atacgatttt gtaacatagt caaatttaaa tttattaata
    13081 tgtttgcctt tatatagctt ttttataata acttttcttt aattagatag tttcttgcca
    13141 taactgttct tggatgtatc tcttcttttt tattgatgac aaacattaaa gtattattaa
    13201 aagaagttaa aagtgcttca tcaagcttcc ctccatatgc taattctctt aatgtatcta
    13261 caactgggaa ttttcttcct tcttcaatta aatcagctat gtaaattatt ttttctaaaa
    13321 gagacatgtc ctctctccct gttgtatgat atttgattgc atttataata tcttcatctt
    13381 ctacttcaaa ttcatactcg catatgtacg ccccaattac actatgagac aaggaacgat
    13441 ttccatcctc tattgggtct aaatatattt catacttatt tacataatcc tctatttcct
    13501 tgtcacttaa atactttgca caatcatgaa tcattcctgc taagtaagct ttttctttgt
    13561 cacatccata aatctcacat aatttttcag cacattttgc aacatttttt gaatggctaa
    13621 gcctccccac tggtagcatt tgatttagtc tttggttaat attttctaag ttcatttaat
    13681 catctccata ttgatatagt ttattttcat ataaataatt ttctacattt tcgggtacta
    13741 agtaacgtat tgacttgcct tcatttagct gttcccttat atatgtggac gatatatcca
    13801 gtgatggaac ataaactgtt ataatgtcag cattatactt cttagttagt ttttctatag
    13861 tctcttgtga ccttaacaaa ctaattcctg gtctagttgc ggctataaaa gtagctaact
    13921 caaagttttt ctttacatct ttccactctt caacactaca aattgcatct gcacctgtta
    13981 taaaataaat gtctgcattt ttgtattttt tctttaagta ttttaatgta tctattgtat
    14041 aagtcctttt ctttctttcg atttctactt ttgaaactaa aaaatcttcg ttgctttcag
    14101 ttgctagaag tgtcatatta tatctatcat acttatctgt agtgatacat aatttatgtg
    14161 gtggatttcc tgatggtata aagattattt tgtcaatgtc atacttatct ctaataaatt
    14221 ctgctgttgc taaatgagca tagtgtatag ggtcaaaagt acctcccaat atacctattt
    14281 tgattttgcc tttatggcga ttaaatttct ctagtttttc agtatttttc aactctgcca
    14341 tcttaactag attttcactt ctcatatttt gtctcctttt aatgttataa tactattgta
    14401 ccattatttt ttatttctta aagcttataa tttgtttttt tgggtatata attatattt
//
LOCUS       DAMJYC010000098        14208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJYC010000098 DAMJYC010000000
VERSION     DAMJYC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.102"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            74..2185
                     /locus_tag="PC472_003152"
     CDS             74..2185
                     /locus_tag="PC472_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2728457.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2383..3348)
                     /locus_tag="PC472_003153"
     CDS             complement(2383..3348)
                     /locus_tag="PC472_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2728458.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3633..4589)
                     /locus_tag="PC472_003154"
     CDS             complement(3633..4589)
                     /locus_tag="PC472_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2728459.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4957..5745
                     /locus_tag="PC472_003155"
     CDS             4957..5745
                     /locus_tag="PC472_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2728460.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5868..6338)
                     /locus_tag="PC472_003156"
     CDS             complement(5868..6338)
                     /locus_tag="PC472_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2728461.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6477..7121
                     /gene="uppS"
                     /locus_tag="PC472_003157"
     CDS             6477..7121
                     /gene="uppS"
                     /locus_tag="PC472_003157"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2728462.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7164..8069
                     /locus_tag="PC472_003158"
     CDS             7164..8069
                     /locus_tag="PC472_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2728463.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            8384..9931
                     /locus_tag="PC472_003159"
     CDS             8384..9931
                     /locus_tag="PC472_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728464.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            9934..10332
                     /locus_tag="PC472_003160"
     CDS             9934..10332
                     /locus_tag="PC472_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2728465.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            10372..12105
                     /locus_tag="PC472_003161"
     CDS             10372..12105
                     /locus_tag="PC472_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2728466.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            12337..13548
                     /locus_tag="PC472_003162"
     CDS             12337..13548
                     /locus_tag="PC472_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2728467.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            13741..14001
                     /locus_tag="PC472_003163"
     CDS             13741..14001
                     /locus_tag="PC472_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2728468.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         5729 a         1491 c         2309 g         4679 t
ORIGIN      
        1 atatgaattt tagatatact taataagaag tatattgtag aaaaaataaa tttaaaaaat
       61 taagggggat gaaatgaaaa aaataagtat attagtatta tcattgataa tgacacttac
      121 tatgtgtagt gtaagttcat ttgctgattc atcaaatgat aaggaaatga gagctgcttg
      181 gatatctaca gtttataatt tagactggcc taagactaag aataatgaag ccaagcaaaa
      241 aaaggaatac acagatttat tggacaaatt aaaaagtgta ggaataaata cagcagtggt
      301 tcaagtaaga ccaaaatcag atgctttata taaatcaaat ataaacccat ggtctgaata
      361 tcttaccggt acacaaggta aagaccctgg atatgaccca ttaccatttt taatagaaga
      421 agcacataaa agaggaatgg aatttcatgc atggtttaat ccatatagaa taactatggc
      481 agatgagagc atagataaat tgccagcaaa ccatccagca aagaaaaatc caagctgggt
      541 agttaaacat ggaaataagt attactacga cccaggttta ccagaagtta gaaagtacat
      601 agtggatagt atagcagaag tagttcaaaa ttacgatata gatggagttc attttgatga
      661 ttacttctat ccaggagtaa gtttcaatga tactgcaaca taccaaaaat atggaaaagg
      721 acaaaataag gataattgga gaagagaaaa tgtaaatact ctacttagag atgtaaaagc
      781 gtctataaag agtataaagc ctaatgttgt atttggtgta agcccagcag gtatatggag
      841 aaataagagt agtgacccaa caggttcaga tacatcaggt aatgaaagtt atgtaggtac
      901 atatgcagat acaagagcat ggataaaaca aggattgatt gactatgtag ttccacaact
      961 ttactggcca attggtctta aagcagcaga ttattctaaa ttagtggcat ggtgggctaa
     1021 tgaagtaaaa ggaacaaatg tagacttata cataggacaa ggtatatata agcaaggtca
     1081 atcgagttat ggtggacaaa atatagcaaa agaaatagta caacaagtaa cattaaatag
     1141 aaagtatagt gaaataaaag gtagtatgta tttctcagct aaagatatag caaatagtac
     1201 ttctatacaa aaggatttaa agagtttata ttctagtagt gaagaaccag ttactccacc
     1261 atctaatgtt aaagtggaga aattaagagg cgacgaaaga tatgacacag cagtagcaat
     1321 aagtaaaaaa ggttgggcta caaattcaga tacagtagta ttagtaaatg ggtactctat
     1381 agttgatggt ataacatcaa caccacttgc tacatcaaat gatgctccta tactattggt
     1441 aaataaggat aatataccta catccactaa gaatgagtta aaaagactta atccaagtaa
     1501 agttatttta attggaggaa ataactctat tggagataaa gttgagagtg agataaaaga
     1561 tacattgtca aatgtatcaa taaatagagt tggtggttca gatagatata gtacatcttt
     1621 aatgatagca aaagaattag taaaaactaa tccagtagaa aaactatata taacaagtgg
     1681 tacaggtgaa gcagattctc tttctatagc ttcaaaagca ggagaagaaa agcaaccaat
     1741 agtattagta tcaaaagata atgtaagtga tgaagtttac aattggatat cagatttaaa
     1801 agtaaaagat gcttatttta taggtggtaa cctaagtata tctgattcag taataaataa
     1861 gctggataag gtcattacaa atgatgtatc taaaaataga atagctggag aaaatagaga
     1921 agaaacaaat ggaaaagtaa tacaaaaatt ctatccaaat gctgaatatt catcaatgtt
     1981 tgtaagtaaa agtaatcaat tggtagatgc attaacatca ggtcctttag cagctaaatt
     2041 aaaatcacca gtagttatgc ttggaaatag tgtaacatct gctcagaaaa ctgctttaga
     2101 gcctaagaaa actactctag tatacgaagc tggagatggt ataaatcaaa acacattaaa
     2161 cactttctta aacttagtta agtaataaaa aagttaattt aaaatacaga gtattttata
     2221 aatgtagatt aaaatgaaaa ttatcatgat aaattaaaaa ataaaaaaga actatatgca
     2281 ttttgtaatc aagtgtacac agcaacttga aaataattat gcgatatagt tcttttttag
     2341 taattaatct tatacttttt aataaacttt atattgaatt ttttattgtt taaactctat
     2401 gtcattaaat ataaaatcgt gtaatatgtc taaagtatct gggtcaaatc taagaaccca
     2461 tccagctttt ccatatatac cacctgtact atgaatttca tcatcaatag gaaattcaaa
     2521 ttgcttaata tttaagtcgc ccatttttaa aacttgagct cctaaagaaa gtattgcatt
     2581 aggcttcatg ttagttttaa catatggaag aactgcatct aataaacctg gatatttagt
     2641 aacaggtaaa gtttccgttt tcttgattat agcttgtata atctgtctct gtctgccatc
     2701 tctagcaaag gctgtatcgt tgtgtctgat acgtgcaaaa gatagtgcct gatatccatt
     2761 tagagtctgt tttccagagt ttctaatgta ttgtatgcta cctttatcat cacttttatt
     2821 ccatttataa gtttcaggaa taaatttatt tagttctcgt atttctcctt tttcaacagt
     2881 aacctcaacg ccaccaatag catcaattat atacataaat gattcgaaat taacacaagc
     2941 ataattttga atatcaatat taaaattctc ttcaattgtt tgtattaata aatcagcttg
     3001 tccataagca taagcatgag taagtttttg tttaccatga ccagggatgt ctacatagct
     3061 atcacgagct aatgatgtaa gtttgatatc attgtgtttg ttatcaatag taagtatcat
     3121 catagcatct gaccttgaag aatcttcatt tggtctagca tctgttccca taagtagtat
     3181 gttaattatc ccatcttcat ttttatggtc attgttatcc aataaatcac tacttatgct
     3241 cgagtcatga atagcagata atttataata aaaatatcca tagacactta tagggaatat
     3301 tactaccaag aaagccagta gtataacaaa tttctttaat tttgacaaaa gtaccccttc
     3361 tttcttcttt ttcctgtata ttttttaata gcatacactt aattatatta aagtattgag
     3421 atatattcaa tacttgtttc agttacaaaa aaaagacaat gatataattt ataagaaaat
     3481 ttatcgacct atatatatag ttaaaagtat atataatata ttttttaaaa gtaatttata
     3541 ttttaaaata aaaacacacc ccataaatac aaagtaattt gcatttaaga ggtatgtatt
     3601 tatcatcaaa ttattggttt gttttggaat ttctaatctt caaccataat atctttaaat
     3661 atgaaatcat gcattgcatc tagttcatat tcctcaaaag gtattacata gcctgcatta
     3721 cctatttttc taccattttc agtagaaaga ggaaactcca tagatttcaa atttaaattt
     3781 cctatactta aaagttcttt tcctaaagat attatttcat taggattcat attagttttt
     3841 acatacggaa gtattgtgtt aactagatta ggatatttag tgactggtaa atccttaaca
     3901 ccatttatta aactctgtat aacacttctt tgtcttctat ctctttcatc tgtactatca
     3961 ttttttctta tacgagaaaa tgcaagtgtc tgatatccat ttaatttttg ctcaccagaa
     4021 tttcttatat atttaagaga acctttattg tcatttttat tccatttata agtttcagga
     4081 ataaatttat ttaattctct tatttcactc ttatcaacag taacagtaac tccacctaaa
     4141 gcatcaacta tatccataaa agagaaaaag tcaactacgg cgtagttttg tatatcaagt
     4201 tggaagtttt cttctatagt ttctattaat aagttagctt gtccatatac gtaggcatgt
     4261 gttaattttt gttctccacg tcctggtata ttaacataag tatctctatt taatgaagtt
     4321 agcttaagac ttttattttt attatcaata gtaagtatca tcatggcatc agaacgagaa
     4381 acttcttctc ctggtcttgc atctgtacct aacaataata tgtttgtgat attttcttcg
     4441 tttttatagt cattactact aagcgcctct gtatttatac tatcatcttg tatagttttt
     4501 aatttagtaa aaacatatcc atatatacta acaggaaatg ctataactaa aagggctaga
     4561 aatataacga actttttgag tcctgacaat aatatcccct actttctaaa ttttttaatt
     4621 acacatagtt aattatatta aaaatttgtt acataagcaa taaaaaacac aataaattag
     4681 cataagtttg tcataatata attattttta tagcatttta tagaagaaag tgtgtagtta
     4741 ttattgattt tattggaata taattgtatg aagacttaaa tttttataaa ctgatgtgta
     4801 tattgaaaaa aatattttcc tgaatgtagt tatagcatct tatgaaaggt gcaaattgta
     4861 atttaaaatt tttagtgtat aataagaatt gaaaattcaa accgataaaa attaataaaa
     4921 aataattata aattaataat ttgggggagg aaatgtatga taaaattaat agcaacagat
     4981 ttagatggaa ctttgttaga tgaaaagagc gaaataaatc cagagtttta taaagtattt
     5041 aaaaaattaa gagagagagg gataatgttt tctgctgcta gtggcaggca gtatcaaaat
     5101 cttataaaaa aatttgaaga tataaaagat gatatgatgt ttatatcaga aaatggaaca
     5161 ctagtagtat ataaaggaaa agaaatatta tcaaatcctt taaataaaga attagtaaat
     5221 gaaatcatag agacaacaag aagtataaaa gggaaaaaaa ttgtaatgag tgggaaaaaa
     5281 tacgcttata tagaaagcaa agatgaagcg tttatacaag aggtcagtac ttattatgca
     5341 aagtttaaaa ttgtagagga tttgacgaaa gtagaaggag acatattgaa gatagctgta
     5401 tttgacttca aaggtgcaga acataataat aatatctact ttgaaaagtt ttctgataga
     5461 gctcaagtat gtatttctgg agtggagtgg cttgatttaa ctgcaaaagg tgcaaataaa
     5521 gggagtgcca taaaaaaagt acaaaaaatg ttagatataa aatatgaaga aactatggta
     5581 tttggtgacc agttaaatga tgtggaaatg atgaaaagtg cctatcatag ttatgccatg
     5641 gaaaatgcaa atgaacattt gaaacaaatt gctagattta gagcaaaaag aaatactgaa
     5701 aatggcgttg tagataagat aaaagaggtt ataaaaattg gatagtcata tgtatagatg
     5761 aaaaagatta ttttgaagaa atcaaaaaaa tatcaatgta attttgataa ataagtttat
     5821 ttatgcgaaa attacattga tatttaataa acatcaataa tagatattta tttttttatg
     5881 atattcagta ttttgttttc tatttgagtt gcattttcag cagaaattaa ataaacatat
     5941 ctacctgcct tctcaattat agtattattt gctatttcag aattgatttt ttctccatgt
     6001 ccttctccaa aaggtgcttg tatcatatct tttttataat tttctagagt tttatagatt
     6061 ttatctacct cgtcagtttt tataacgata atatcttcta gagataattt ttcagaagaa
     6121 ttaattacaa aaccttgaat tatgtcttcc tctacatcat taaaatagtt aaaatccttt
     6181 gcatcaaaga tttttgattc ctttataagt aaatcagaag attttaatgc tttttttatt
     6241 tttgatgtag aaacgttttt gtaatcacca cttgaagaac aactaaccat tcctaatgat
     6301 aagattatcg aaaaaaacaa tattaaaatt tttttcatga taaacctcca agttttttaa
     6361 actctgtctt aaattagttt actacataat tatataaaaa taaatgtatt taatgttaca
     6421 gttttgatta ataatgtaat attttgataa taatacctat tgagaggtga ttagaaatga
     6481 gaataccaaa acatgtagga attataccgg atggaaatag aaggtgggca agtcaaaatg
     6541 gtatgacaaa agataaagga tatgaaagtg gaatagatcc tggactagag tcttatagat
     6601 tatgtagaga tttaggctgt gaagaggtga ctttttatgg atttacaact gataatacaa
     6661 gacgtccatc agtgcagaca aaagctttta caaaagcatg tattgatgct gcaaatatat
     6721 taatagatga aggtgctgct ttattagttg taggaaatac aaaatcgcca atgtttccag
     6781 aagaattatt accatatact aatagaatta aagccaaaga aggtcaaata aaagcaaatc
     6841 ttctggtaaa ttatggttgg tattgggact taaataatat atcaggagta gaaaatgtaa
     6901 ataaaaataa tatacaggat tacatacagt catttgatgt atccagacta gacttgataa
     6961 tcagatgggg tggaagaagg agattaagtg gtttccttcc tgtacaatca atttatgcag
     7021 atttttatgt tatagataat tattggccag attttaagag tgacgatata aaggacgcat
     7081 ttagttggta tgatacacaa gatgtcactt taggtggata attaaaaata ttctgattta
     7141 aggttgtaga gtgttttaaa ttattgaaaa aaaaacttct tgatggtaaa ataacactaa
     7201 taatatgtaa aagtgtaaaa atttatacca agaagggtga agaaatgtca aaaggcaata
     7261 ataacaataa ttcaagaaac aaatctaaaa agaccagtca tctgaataga aaaaagcgaa
     7321 aattaaataa gaaaaagtta gcagtgttga tttgctttac agttttattt ttatttattg
     7381 catttaaggc tactcaaggt gtagtagctt tggtaaaaag tatggataaa agtaataaaa
     7441 ctaatcaaca acaaaatgtt aactcagaac aatttgacct tggtaatgaa gaggaaaata
     7501 aaaagaaaaa atatactgta tttatagacc caggtcatgg aggcaatgat aaaggaactg
     7561 aaagcaagac cagtaataga tatgaaaaag acctaaatct acaaattgca aaaaaattag
     7621 caaataaatt atctaaacaa aaggatattc aagtagtagt atccagaact gatgatacat
     7681 atatatcatt aaaggataga gctatcttag caaataatag ttctgctgat gtgcttgttt
     7741 ctattcattt aaatgcagag aaaaatggta atacagcaac aggtatagaa acttggtata
     7801 ggaataaagc tacagatggt tcaaaagaac ttgcacagac agtacaaagt actatagtat
     7861 cctatgttaa agttagagat aggggaatag ttgaaaataa ctttgaagta cttagagaat
     7921 caaatatgcc tgctatatta atagagtgtg gttttttaac aacaccttca gaggaacaaa
     7981 aaataataaa tgaaaaatat caagaccaac ttgcggaagg aattgttcaa ggtgtacttt
     8041 catacctaga ttctaaaggg aataagtaag ttaactttac tttaatgtga ggataaattt
     8101 aaaagtttaa ctgtgtaaat tgtattaact ttatttcgta atgttgcaaa cattgtttga
     8161 atttagaaac tatgaaatta gaaattattt tatctatatc taatttcata gttttttttg
     8221 caaaaaattt atagttataa aattttaaaa aataaattta gtataatgta atgtagttat
     8281 ttatatgtga ttttaacttg cattgatttg ctatcaattg tgctttataa ttagtatata
     8341 taaagtttaa ctgtaagata atttaaaaaa taggggaatt tatatgaata aaaatagcat
     8401 gacaagaatt tacagtttaa ataaaataat ttttattata attggaattt taggaatagt
     8461 tttactcaca agacatattg aggtagatat taaagtttta attttaattt tattaacctt
     8521 gtttagtata aatgtattta tttcatatct taaaattaag ttgtatgagg ataaaattaa
     8581 tttaggattg ataaataatt cagataagtt tataaatatt actaaagatg agaacttcct
     8641 aaaacatgtt caaaattata ttatcagtaa tgaaaaagaa aactgtgtaa tggcttgttt
     8701 tgatttatgt agactcaaac ttataaatga tatatatggt tatgagctag gggatgaagt
     8761 gttaaataat attttaagta acttaaaaaa ttattttgga aaagaagcta tttatggaaa
     8821 gttaaaaagt gatgtttttg tattaataat aaagcttgaa aatagggaac agaagattcc
     8881 ttatttagta aatcttatta agaataaaat tgtgatacta agtcagattg acagttttaa
     8941 aatggatata aatattgagg cttctattgg aatttataaa tttaacaata atgaaattga
     9001 tataaaaaaa gctatagata atgctgatat ggcaaggctt aaatcaaaag gactaaagca
     9061 catagagtat gtagaatttg ataatgctat ggaagaagaa atacaatcaa ttatgaaaat
     9121 agagagagat ttattcttgg ctattaaaaa taaagagttt gtaattcact atcaacctaa
     9181 agttgattca agtacaggta acattatagg ttcagaggca ttgataagat ggttgcaccc
     9241 atcattaggc atggttggac caaataagtt tatacctata gctgaaaaaa atggtctgat
     9301 aaacaatatt ggtagatggc taattcaaga agtttttatt accattaaca agtggatttc
     9361 agaaggtata aatgtcttac cagtctcaat aaatctttct agagtagaac tatataaaaa
     9421 tgatttagtt gattttttta aactgatgtt taatacatat aatattccaa aagaacttat
     9481 agaattagaa attacagaaa cgacagcact aagagatgta aaatttataa gtgaaaggct
     9541 ttatgagatt aaatctttag gtatgaatat atcattagat gattttggag taggtaattc
     9601 aaattttatc aatcttaagg ggataccact agatattata aaaattgaca ggtcacttat
     9661 attggatata gttacaaata caaaaacaaa gtttatggtg aaagcaattg ttaacctttc
     9721 acatgattta aacgtaactg ttatatgtga aggggtagag gatatgcatc aggtcaaagt
     9781 cttaagtgaa ctgggatgta atttcataca aggttatgta ttttttaaac ctttggatga
     9841 gatgaattat aaaaaacttc ttactgacgg aagttatata aatctgaaag atactctttt
     9901 agataaatgc atgacagttg aggaggagta aggatgattt cagaagatgt agattattca
     9961 ataagaatca taagagctat atttaataat ggagaagata aaccaataga tgctaaaatg
    10021 atattaaaaa gtgaagatat accaatacta acactataca gactgcttag aaagctttgt
    10081 gcaaacaata ttcttacttt taacaaagaa gatggcaaga catacatgtt aaatgtgtca
    10141 ttggatacaa taagtctaag aactattgta gaagtaattg atggaaaaat atatataaat
    10201 aaatgtacga gatatgtatc taattgcagt aattgcttaa aaggttgtaa agtaaatcgt
    10261 gaattgctag aaatagaaaa agaaatttat aaacttttat cgagaaaatc tataagacaa
    10321 atactagaat aaaaaatttt cagctatgat aataattgga ggttttcggt tatggatgat
    10381 atatctcaag ataattttct actgtctaaa gagtatgaga atagcttaga tgtagatact
    10441 aaaaaagcaa gtggaatata ttatactcct aaaataattg tagattatat tgtaaaaaaa
    10501 actttaaaaa atcacgatat aattaaaaat ccatatccac gaatacttga tatttcctgt
    10561 gggtgtggaa attttctttt agaagtatat gatattttat atgacttatt tgaggaaaat
    10621 atatatgaat tgaaaaagaa gtatgatgaa aattattgga ctgttgataa catacaccgt
    10681 catatattaa actattgtat atatggtgct gatattgatg aaaaagctat aagtatatta
    10741 aaagatagct taacaaataa aaaggtagta aatgatttag atgaatctga tataaagatt
    10801 aatctatttt gttgtgatag tcttaaaaag aaatggcgat ataagtttga ttacatagtg
    10861 ggtaatccac cctatattgg tcataaaaaa cttgaaaaga aatataaaaa atttctccta
    10921 gaaaaatata gtgaagttta taaggataaa gctgacttat atttttgttt ttataaaaag
    10981 attatagata ttttaaagca aggagggatt ggcagtgtta taactccaag atatttttta
    11041 gaaagtctat caggtaaaga tttaagagaa tatattaaat caaatgtcaa tgttcaagaa
    11101 atagtggatt ttttaggtgc aaatatattt aaaaatatag gggtatctag ttgtattcta
    11161 acttttgata agaaaaaaac aaaagagaca tatatagatg tatttaaaat caaaaatgag
    11221 gatatatgta taaataaatt tgaaacattg gaagaacttc taaagagtag caaatttgaa
    11281 cattttaata taaatcagag attgctatct gacgaatgga tacttgtaaa taaagatgat
    11341 gaaacttttt ataataagat acaagaaaaa tgtaaatata gcttggaaga tatagcaata
    11401 agttttcaag gaataattac agggtgtgat aaagcgttta ttttaagtaa agatgatgta
    11461 aagttgaacc ttgtagatga taaattttta aaatgttgga taaaaagtaa gaatataaat
    11521 aaatatatag tagataaaag tgaatatagg ctaatatact caaatgatat agataatgag
    11581 aatacaaata aaaggatact agatgaaatt atagggctat ataaaacaaa attagaaaac
    11641 agaagagaat gtaaaagtgg aataaggaaa tggtatgaac ttcaatgggg tagagaaaag
    11701 cttttctttg aaagaaaaaa gataatgtat ccttataaat caaatgaaaa tagatttgct
    11761 atagattatg ataacaattt ttcaagtgca gatgtatatt ctttttttat aaaagaagag
    11821 tatttagata aattttcata tgaatatcta gttggaattt taaattccag tgtttatgat
    11881 aaatacttta aaataactgc taaaaaaatg agtaaaaata tatatgatta ctatcctaat
    11941 aaggttatga agataaggat ttttagggat aataattatg aagagataga gaatttatca
    12001 aagcaaataa tatcaatttt attaaataag tctatagata aaggaaaagt agaaaaattg
    12061 cagataaaaa tggataactt gataatggat tctttaggta tataagcagt tgtaagtatt
    12121 aaaaaattat aatcttttaa ttgtttattt aaatttagaa ataaaaagtt agccatataa
    12181 aaaatattaa aaatatatac ttttcaatat ttttttgata aaataagaat gaatttgtgc
    12241 aattttattt ttctccaata actaaaaata gttttttgaa ttaatatttg cttaagaata
    12301 aaaatcagaa acgtttttga tatagtaggt gataatatga aaggattagt tttagaaggt
    12361 ggaggaacaa aaggagccta ccaaataggt gcatataaag ctttaagaga gcttggtata
    12421 gagtttcaag gagtaactgg cacatcaatt ggtgctttaa atggagcata tataattcaa
    12481 aatgatattg aagtaatgga agatatttgg ttaaattatg actatactca ttttatgaat
    12541 attgatagag aaacctatga gaggtttaaa aatgtagatt tcactactaa aaatctcaat
    12601 gcagtgtttg agcttataaa taaagctaga aaaaatgaag gtatagatat aagcccatta
    12661 agacaattaa tggagacaac attaaatgaa gacataataa ggaaatcaaa aaaagacttt
    12721 ggacttgtta cggtctggtg ggataaaaaa attaatccat atccattgta tatcgaagat
    12781 atacctaaag gaagattgat tgattattta attgctagtt ccagtttgcc tatttttcaa
    12841 acgaacatga tggatgataa gttgtatctt gatggaatgt tttgtgataa catgccaata
    12901 ggactcttgg aagacaaagg ttatgaagat gttgttgtaa ttaggttgat tgatgatttt
    12961 attgggaaaa tgaatctaaa tcgacatcaa aatataaata taaaaactat aataccatca
    13021 gaatatctag gaggttctct taataaagat agagataatg tagaaaaaaa catccgacta
    13081 ggttacttag atactatgaa agcatatgaa agatgcactg gaattaagta ttattttgat
    13141 gtagagtata aatataatga agaatattgt tttgataaat ttagaagtat gagcaatgaa
    13201 acaatacaat atatctgtaa tttattgaat ataaaaagag atgcttcact tagaactgtt
    13261 atggaaagtg taataccaaa gcttggtgag gtgcttggat tgccaaagga tttttcatat
    13321 aaagatttgt tttactgtat atatgaaaag aaacttgaag aaaataatat aaacaggata
    13381 aaattatatg attttaacaa ggttatagat gttgtaaata caaatattga ttctaataga
    13441 aatttagctt ctaattttga agttagacct aaaattagta ttaaaaagaa caaaaacaca
    13501 aaattattga caaattgtat tataaaagac ataaaaaatc aaagttaaat aaaaaacaaa
    13561 aaaaaatttg taattatact cgaaaaaagt gtatattagt agtataatat ttttgtagca
    13621 ctagtgggaa caaaaaatga ccataggaca aaaaagtacc acttagtacc attaaagtaa
    13681 aaaacaggaa aacaatttta aaaaatataa aatgtaaagt ttataggagg atttaaaaaa
    13741 atggaaaaag tagttagtat caaaaatgca agtggattac atgctagacc agcaggaatg
    13801 tttgttaaga aggcagctga atttaaatca acagtagagg ttgtagcaaa agacaaaact
    13861 gtaaatgcaa aatcaataat gggaataatg agtttaggtt tagctcaagg agaagaatta
    13921 aagttagttg taaatggaga agatgaagaa gtagctttag ctgcaatggt tgaattagtt
    13981 gaaagtggat ttggagaata gatataataa ttttttaaaa atttgatatt tagaatctaa
    14041 tattacttaa agtaaagtaa cagagaagta ttaaacatat acagaccaac atttgtagat
    14101 atttaatttc tttgttactt tcatgtttat ttaatcagaa gattaagact attgttaatt
    14161 gggtataaaa atataaatat tttagaaaat gaaataagat atttttaa
//
LOCUS       DAMJYC010000099        14132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJYC010000099 DAMJYC010000000
VERSION     DAMJYC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.103"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            438..2672
                     /locus_tag="PC472_003164"
     CDS             438..2672
                     /locus_tag="PC472_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728469.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPNDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2703..3548
                     /locus_tag="PC472_003165"
     CDS             2703..3548
                     /locus_tag="PC472_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2728470.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3788..5521
                     /locus_tag="PC472_003166"
     CDS             3788..5521
                     /locus_tag="PC472_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2728471.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5913..6736
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5978..6006
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7016..7168)
                     /locus_tag="PC472_003167"
     CDS             complement(7016..7168)
                     /locus_tag="PC472_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728472.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            8292..9647
                     /locus_tag="PC472_003168"
     CDS             8292..9647
                     /locus_tag="PC472_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728473.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVFSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9765..10433)
                     /locus_tag="PC472_003169"
     CDS             complement(9765..10433)
                     /locus_tag="PC472_003169"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2728474.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFEKALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(10500..10928)
                     /locus_tag="PC472_003170"
     CDS             complement(10500..10928)
                     /locus_tag="PC472_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2728475.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            11035..11628
                     /locus_tag="PC472_003171"
     CDS             11035..11628
                     /locus_tag="PC472_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2728476.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11747..12682
                     /locus_tag="PC472_003172"
     CDS             11747..12682
                     /locus_tag="PC472_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2728477.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEIMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(12953..13897)
                     /locus_tag="PC472_003173"
     CDS             complement(12953..13897)
                     /locus_tag="PC472_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2728478.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFCKSNKLKQIEKTESI"
BASE COUNT         5336 a         1869 c         1826 g         5101 t
ORIGIN      
        1 taaacttaat tttaataact aaaattaatt atgcaaaatt attaatatgg tattaaatta
       61 tgtataaaaa gttaattatg aataaaataa ttatgccttt gaaaaatcat taaacttaaa
      121 attaattata aaaaaactct accttactaa ctatacagct cacaatctat ctatttatga
      181 aaaaatatga tataattgca ttcaaaaagg aaatctttca atatactaga atttaagcat
      241 taactaattt tatctaatat tattactaaa ctaaaaaata tttgtatttg tagcattgga
      301 gcaagtaact taatgtatat tcattttaaa tacttattta aaatttagca tttaaattat
      361 ttacaaatta acatttaaat acttctagca taaatacagt tgtcatcaaa atttaaagta
      421 tcttatggag gaataccatg caaaatcata attatataaa aatgttaaat aaaaaggtag
      481 aagtattatc taaggaatta gatagtagaa attttaaatt aaaaacctta aatgaaatag
      541 attttttttc taaaatacca aacagagaat ttatgtacca atatattgat gactgtattt
      601 cgaaaaatat acatatagga atcatgttat taaatatcga taactttaaa ttatttaatg
      661 atatatatgg atatctagtc ggagaaaatt tattaattac tctaactcaa aaccttttgc
      721 actttattcc agaatcaaag ggaatagtcg cttatttaaa aggagaaggt tttttaattg
      781 tacttccaaa tgtatcaaat aaagagttaa ataatatggg tgcaactatt atagagagcg
      841 cccgagaatt aaaaataaaa cttcctaatg aaaaagaaat atactccaat gttacattta
      901 gtattggcag cactatctgg aatggtgaac caaacatgac tactttgatg cttttcaacc
      961 aagtatatag tgctttaaaa aaggctaaaa gtgaaggtaa aaatagatac attctatttg
     1021 attttaaaga taatagtttt tacaataaat tagaaaatga taaggagttc ttatcagaaa
     1081 tatcacatgt tctacaaact aaacaaattg aagtatttta ccaaccactt tataatataa
     1141 aaaataatac tatagttgga tgtgaggcac ttgcaagatg gagacaccct attaaaggac
     1201 ttcttactcc agataaattt attcctttat ttgaacagtt tggtctgata acggaattag
     1261 atttgcattt atttgaagag aactgtaaaa atataagaag atggattgat aacaatgatg
     1321 tttttgttcc aatttgttgt aacttttctc gtcttcattt ttttaaccct aattttccaa
     1381 gtatgctaaa aggtattact gaaaagtaca gcgtctctac atcaaatttt agcattgaaa
     1441 taacagaaaa tatattaata gaaaatacaa atacaattat tcaacaacta gctgaacttc
     1501 gttcttatgg tttttctgta tcaatggacg attttggttc tggatattca tcttttggta
     1561 tgttgcaaaa tctacctttt gatataatca aaattgatag gatattttta tctcataatt
     1621 taaatgattt taaaaataca accattttgt actctataat aaacattgca agagctcttg
     1681 ggatgcttac agtatgtgaa ggtgttgaaa cagaacaaca tgtagaattt attaaaagta
     1741 taaattgtga tatcgcccaa ggttattatt atgcaaaacc catggagtgc aaagaatttg
     1801 aaaaattact atctaaagag aataattcag agaaaatgtt tataaaatat tctgatatag
     1861 gaaaatcttt aatagagaag atatttgatg gattttttat tatgcaagat atgaaaaaac
     1921 taagagctaa tgtcactgaa gacattcaat ggtttgattt ttacaacaaa actgaaatta
     1981 catcttttcc taaagtaata gaacattttg aaaaacatat taaaggtaaa aaattttcta
     2041 ttctttacaa aagtataacc ccacataata ctgaatcaga ttcattatta attagtggag
     2101 aagccgtttt atcagatgaa gcttctgaac atcaaaaata tagcaatttc tactttagtg
     2161 caaattgtat aatttcagaa aataaattac tcttagcaaa attacatttg gatttaatta
     2221 aaactgctgg ctatataaat gaatttgaat catattcaat acctaaaatg acaaatacac
     2281 atcttgatga aagtaatatt ttatcacaat tttattcaat gttgcctgta ggtataattc
     2341 gctacgattt atttggtgat atgattatta cttatatgaa tgaagaaatg tttgatatta
     2401 taggttatac aaaagaacag ttttttggtg agatgggcgg aaatttaaga gcaattgttc
     2461 atccaaatga tttagacttt gtttataaaa aaagtttgga tatgattgac acaaataaaa
     2521 ttgaaccatt tttatataga ttcataaaac gagatggtca aattgtcaca gttatgtata
     2581 cacaatgtaa cctttctgca attgatggaa ggtcgattgc ccagggtatg tatgtagatg
     2641 taaataaaat aaaaaattta acaaaaatct agtacaaagt attttatttt ggagaaatta
     2701 atatgtttaa agaaattttt ttaagaactt ttccaggatt tctaattata agagattcta
     2761 actacagaat tatatttata aatgataatt taaaaaattt aattaaaagt tatatgcaag
     2821 ataatccact tggaatgact aatatagaaa tagctaagaa actccctgat aatattgcta
     2881 aattttttac ggattctcac aatattcaat tagattggga aaaaaattat ccttatgata
     2941 aaatttcaaa ttggatttta gagtttaaaa aagatactac aagctattgg aatgttttag
     3001 aatataaagt agatgttgat gaaaaaacct acataattac tatggccaat gatataacaa
     3061 aactctatga agaaaataag agaaatcttc actactcaat tactgaccca ttaactggag
     3121 catataatag aaaatattta aatgatagat ttgacatatt tataggagat tatattgtac
     3181 taattgactt agataatttc aaaatgataa atgactatga aggtcataat gtaggagaca
     3241 agattttatg tgattttgtt tcacttttaa ataaagaatt aataaattct acttctatta
     3301 taagattagg aggagatgaa tttattgtta tattctctag tgatgttgac aaaaattatg
     3361 tttattcaca gctagaagcc ttaagagaaa attttttaaa agtattctct aagtataagt
     3421 atctttcatt tagttatggt gttgatacag taaaaagaaa tttaaaattg acaatagttg
     3481 aattggataa aaaaatgtat aaaaacaagg agaaaaacaa gaaaaaattt gataaaaatg
     3541 attattaata ttgttatttt aaaataaatt aaaattatct ttcttttttt aaacttgtac
     3601 attagtttat aaatatgtat ctatacaaga tattgacatt aagtactgaa gcatatattg
     3661 tttcagttct tttttgttaa aattaactta tttaacaaag atatcatagt ttaacaaagc
     3721 ttttaattta aatatattga tgtataatat aattaaaata taatagcaaa aagaaaggag
     3781 gaatatcatg agcatactcc taaaaaaagc acccaaatta gcaaaacata ttataacaag
     3841 tttttatata aatcgggata ttgatgaagt attaaaatac ctttgtgaaa atgttacatg
     3901 gatcggacca ggagaacagg aatttctcac ttcttttaat gaaataaaaa attattttta
     3961 tgcaggtcaa gatgaaattc cttcttgtga tataaataat gatatatttg aaactgtaag
     4021 tgaagatgag aatagatgta tggtattagg aagatatact gttcgaacta aagaaaatgc
     4081 tcaaatgatt ttagaggtaa atcaacgttg tacatttgag ataattgagg atagaggaaa
     4141 attattaata aagcacatgc acatatcaaa cccttatggt gaaatgcaac ttgatgaata
     4201 ctttcccact aaaattggaa ctcaaagtta tgattatttg caaagattac taaaagaaaa
     4261 aacagaagtt attgaaatga ttacaaataa tatcaatggt ggcttaaaag gtagcaatga
     4321 tgatagtaca tactcatttt tctatgtcaa tgagggattg cccaaaatat taggatacac
     4381 atatgatgag ttcatggaaa tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga
     4441 cttacctaaa gcactagaag attgtgaacg atgttttgca aaaggaccta catattcatc
     4501 tgaatataga atccgaaaaa aagatggaac attaatgtgg gtattagatt ctggaatgaa
     4561 atcgttaaat agtgatggca tagtaaaaat taatagtatt attactgaca taacccaact
     4621 aaaaaacata gaatctgaac taaaattaga aagagagcga tatcgtattg ctttgcaaaa
     4681 tattacagat atcatgtttg agtatgatat tgaaaacgat acccttataa aatatcaacg
     4741 tgtagaaatt gacaaaaaaa ttgaattaga aaattttgaa acaaaaaatt actccaaact
     4801 attagaatct ggtaaaatta ttcaccttga tgatattgga aaactactag aagtacttca
     4861 tggaaatctt cgtgaaacaa ttgagataag acaagtaaat tcattaacta aaaatgaatg
     4921 gagatggatt cgtgttcaat gttcagttat atatgatagt gaccataacc ctatcaaaac
     4981 aataggcgtt ttaaaagaca taactgaaga taaatctaaa ttagaaagtt taattaatca
     5041 ggctcaacgt gacccactaa cacaattata taatcaaaga gtatcacaaa atcttattga
     5101 agagtatcta tgtagttctg attccaaaaa taatgatgct ctattagtta ttgatattga
     5161 tgattttaaa actgtaaatg atacttttgg gcatctagag ggaaatgaag ttttagttgc
     5221 tgtatcaaaa atactattac ataacacaca tgataaagac atagttgctc gaattggtgg
     5281 agatgaattt acaatattta taaaatcatt aacaaaggat ttaataataa aaattactaa
     5341 tgatatttta aatgatgcga gtaaaataaa agtaaaggac aatcataaaa taacattaag
     5401 tattggtatt gccttcactg atgattcaac taaattatat aaagatttat tttctaaagc
     5461 tgataaagct ctttatctat caaaagcaga tggcaaaaat tgttatagta catacgaata
     5521 ataaagctat ttatacgacc aagaatgaac ctttaaattt aatttcatag tctaacaatc
     5581 aaagagttga agctaatctt atagctagtc aactagttcc tagaaaaaat ttagacaata
     5641 tatttattga tactgctcat ttttctaaag agcaaattta tataaaaaat taaactttta
     5701 tgtacataca gatactaatg ataaacagtt atcattagta tcttctatct taaaacaaaa
     5761 tgataattta tctatataaa aaagtgcttt gttgacagag ttcacttttt tatataggta
     5821 cagaatttaa gtataaatac atgttttatt ctatacattt tagttaatgt aaaatttctt
     5881 gattgtactc tctaactttt caacattata ctatttatat accacttggt taatataaaa
     5941 ctattattat agtgtcgtgt ggagggcggt gtgacttatt tacataccac ttagttaata
     6001 taaaactgtt ttctgaatct ttttttgtat ctataatatt tttatttaca taccacttag
     6061 ttaatataaa acaaatccta attgggtagt tatccaaaag actataacat ttacatacca
     6121 cttagttaat ataaaaccta gcttttattt cctctgctgt cttatcaaca ttattattta
     6181 cataccactt agttaatata aaaccttttg ttttaggatg attatataat attacaaatt
     6241 tatttacata ccacttagtt aatataaaac ttttttaatg caaatttata aaaaaggggg
     6301 tggatagaat ttacatacca cttagttaat ataaaacttg aatttgtaaa tagttagtta
     6361 taaaaattaa aagatattta cataccactt agttaatata aaaccacaca tctgatacta
     6421 aaaaatataa tgctaaagat aatttacata ccacttagtt aatataaaac ttgatgttgt
     6481 aatatgtcca gaaaaaaagt ttaaaactaa tttacatacc acttagttaa tataaaactc
     6541 attgtactcg ttaagtttat ttgtgaaaaa atctaattta cataccactt agttaatata
     6601 aaacttacag taacaaaaaa attagatgat gttgacaatg atttacatac cacttagtta
     6661 atataaaacg atttttctgc ttcgactggt gttattgaga cttttttatt tacataccac
     6721 ttagttaata taaaacccca aaataaactg ggcattttca atgcttacac atgtgcaact
     6781 ctcttaaatt tgcagtgaac catgagtagt gcaaatgata aagcttatca catgccctca
     6841 acaccttata tttcaagcat taagccctac tttgtaacaa atatcgctca ctgcaaaact
     6901 atttacattt ttattatatc ataaatatat tatttttgaa tatctgtacc aatttgtggt
     6961 ataataaaaa caagaagaac tacaatctat ttagacatag agtgaagttc aaatactaat
     7021 gtttattctt tttgaacttg atttttagtt caaaactaaa gtcgctcctg ccagagtggc
     7081 tttttacttt tttaaataat ttactattta agtaaactat taaactggca actaaacttg
     7141 ctagtacacc ttgtaaaaaa ttatccatac atactcacct cccttctata cattgggagg
     7201 ataatctttt gtatgaactc cactctataa attgtagatt acatcttctt gctaaaaatg
     7261 ttatagcata taattcttac atattttacc tatatattac ttttctaaag attcgtctat
     7321 atatttctgt atacatctac tctatcctcg atttgttaca accccaatca aaaaagtagt
     7381 tcaacttcta agttttatac aaagcaggaa actcaattat tattagaaaa atcaaaaaat
     7441 actcctatag gtataccaat ttttttgatg ctaactcttg gattacgttt tggtgaagct
     7501 gtatcactta tatggtcaga tgtagacctt gataaaaaat ctcacatgta aatcaaacat
     7561 tagtttatgt atacaataaa ataatcttca aaggccctaa aacccctaga ttcaaaagaa
     7621 gattatttgt tcctgatgaa cttataagtt tgtttaaagg ggaaaaatta aaacaaaata
     7681 agttaaaatt acagggtata ttgaaaaatg aatttgattt gatatgctta aataaaagat
     7741 tcaacccgtg agttcaacca acttttgttg ttcattttaa aaaactatta agagataata
     7801 atcctagaga tataaaagta catgaattaa tacatgcaaa tgcgactttg atattacttt
     7861 caggtactaa cataaaagct atttataaaa tattagtaca tgcagatata aaaatagcta
     7921 tgaatatata ttccatattc tcacttatta gatgaaatga ataaagaaac atctgaaaat
     7981 ataagcaaaa tactattcaa ataatgttca gttttatgtc aattaaatgt cactcaaagt
     8041 gctttttttc catttgtcag ttaaatgtca cttaaagtac ctttttccta tttgtcagtt
     8101 aaatgtcagt aatatataaa taagatatat ttataagacc cttaaaatca ctattattta
     8161 ataaaattta taattatata ctatttgctg ttgacattgg tattatacca tagtttatca
     8221 tttatattag aataaaatta ttaattaata gtttcgtaat tttataatag atttacaggg
     8281 agtgataata aatggataca aaaatatcta taaacgaaaa catgagtcta ggtaaaagat
     8341 tttttaaata cctagcacca tctgtagttg caatgtgggt tttctcacta tatacaatgg
     8401 ttgatggaat atttgtaagt aaaggagttg gagaattagc tttagctgct gtcaatatat
     8461 caatgccttt tattaatttt atatttgcag tttctctatt attttcaact ggtgcatcta
     8521 ccataattgc aatctatctg gggaaaaaag atattaaatc tgctaacgaa gtcttttcat
     8581 ttaacttagt atcaataatt atattatcaa taattatatt agctataact ttttttaatt
     8641 tagatagatt agcactattt ttaggagcta cagaatctac aattggtatg gttaaagatt
     8701 atttaggaat aataatattt tttaacggat tctttatagt gtcttattct ttagaagtta
     8761 taataaaaac tgatggcttt ccaatattag ctactgttgg tgtaataata tctgctctta
     8821 caaacatcat acttgattac ttgtttgtaa ttgaatttgg atggggtgta aaaggtgctg
     8881 gtattgcaac tggtttgtct caagtctttt ctactatttt cttcttaata cattttttaa
     8941 gaaaaaactc tactttgaat ttttcaaaat ttagaattga ttttaagaca cttagaaaaa
     9001 ttgtatttat aggttttcct gattcaacta ctgaattaag ttgcggtata gttgttttac
     9061 tttttaactt aagtcttact aaatatattg gagaaaatgc tcttatttat tatagtgtca
     9121 taaattatat aaatacatta gtacttatga ctatgatggg tataactcaa ggtatgcaac
     9181 cacttactag cttttattat ggtgcaggaa atatagataa tgtaaaaaaa ttattaaaaa
     9241 tggggataaa agccacaata atagcatcag ttgctgtatt tgcaatttgt atggcttttt
     9301 caggaccaat agtctcttta tttatacatc cagaagaaac aatgctattc aatgaaggag
     9361 ttcgtgtatt taaaatattc tctatatctt tcttattggt aggaattaat gtaataattt
     9421 ctggattctt tgtatcagta gaaaaaccat caatttctac tgttatatct cttggtagag
     9481 ggcttgtaat agttgttttt agtctaattt caatgatact aatatttggt ggacaaggaa
     9541 tatggatgac tactattgtt tctgagttta tatgtcttat tttatcttta gtttttctaa
     9601 aaaagaattt ttctacatta gactctaatc taaataaggt tgcttagatg ataagagtag
     9661 caatatatga aaatatctaa ataaaaaata cctattatac aatcttgcaa atattaaacc
     9721 tttgcaaaaa ttaggattat ataataggta ttttatttat gtaattattt tatttctaaa
     9781 tatccatagc catcttttat taaagttcct tttacttcat attctacagg ttttttaaat
     9841 attggaccat ctacgacaaa tgtatgatac tctccatgtt ctccacatgg gtctgcacca
     9901 gcatttttta tcttctccac aagttctcta gttaattttt ttcctaaaaa ttcctctcct
     9961 aagtgcttta agtttacttt tgttacaaca ctacaaaatc cactgtctat aaattcatat
    10021 actaaatcct ctctactctc ttgccataat ggtaaatcta ctttcatacc aactgcttca
    10081 catctatcag tatcccattt tctatgactt tctatatcta tatctccata tgcacatatc
    10141 gttgctccca tgtttttagc ttttattaaa gccttctcaa agctctcttc atatccttct
    10201 acaccacact cagcaactaa taaaggtaat cctatattgc tacttacttg cttaagcata
    10261 ttatattcta aattatgtgt ccatgacttt ccatgttctt cacttatagt tgtcaagagt
    10321 gccacaggct tatatccttt tttcaacatt cgatttaatg ctaatatact atccttacca
    10381 ccactaaatg acattacaaa tttttcatca gccaatttta acacctcttt catctaactt
    10441 tgttttatta acataattat accaaatttt tgagttgcat agcaaattat attaaattgt
    10501 taattcacta taaagaatgt ccctaatatt aaaagtataa cccctatgta tttgtataaa
    10561 tgcattgttt cttgtcccaa aattacgcta tcaataaaaa atccaactaa aacttgggat
    10621 acaagagtta atataagtgt atttgatact cctaaatttg gaattgcctt tactgtaaaa
    10681 tatattatta gacctccaaa tactcctcct aataacaact tataattaaa accatttata
    10741 gatactagac actttaagtc acctgtaact attatacttg ccaaaaaaaa tattgctcct
    10801 acagttaagc ttataaaggt ggccactaat gctgttgtac tttttcctag ctctccattt
    10861 ataaaagctt caagagctgt agaaccacct gctaataatg caaatattat tgcccataat
    10921 tttgtcatgg acaaacctcc ttctatataa ttctattaat agaagtttat gatacaacct
    10981 attaattaat tactaagtat agtttaattt ttatacacta ggctttattt ggatatgaat
    11041 ttctgttata attgttctat cgattttgga ggtgatttta tgtacacaaa ttttaatatc
    11101 gattttaata actttaataa aaaagaaaat gctattaaat ttatgttaat gggagtatta
    11161 ctcattatat taggattatt atgccttaca tttaagacat taggtataaa attaatttct
    11221 tggacttttg gtattgcact tttattcttt gcatatttaa atttaaaaaa tattaatgaa
    11281 ttaaaaagat atgctactaa agaagaaatt aaaccctcta ttaatatcca atgggttcta
    11341 ataattgcat gtatattact ttttgtattt ccgcaaaaaa tacaaagcat attttcatta
    11401 tttttaggat tttatttaat ctttaatcaa ctagtagcac ttgtaaatag taagaataat
    11461 ccttattcta aatttacaac ttggaatata gttaagattc tatttggtat atgccttata
    11521 ttatctccac tttttttatc tagatttata gtatcaataa tgtctttttt cataatatta
    11581 tttggactag ttctattttt ctctggaaat acagcgagaa aatattaatt attttctcgc
    11641 tgtattttta tattacttaa aatagaattt gtttaagtta tctatttgtg gtaatatgtt
    11701 ttttgtacaa gctcagcgta caattttatt agaaataggt gattttttgg ataaatttaa
    11761 atatgctttt gataataaaa gatatcatac ttggaattac tatcttagaa atacttttgg
    11821 agaaaaagtt tttaaggtat ctataaatgc aggatttact tgcccaaaca ttgatggaag
    11881 tcttggttat ggcggatgta cttattgtag caaagaaggc tcaggtgatt ttgctggaaa
    11941 ccctaaagat aatcttatca gtcaatttta taatataaaa gaaataatgt taaaaaaatg
    12001 gcctcatgca aagtatattg gctactttca agcctataca aatacgtatg ctcctcttga
    12061 agttttaaaa gaaaaatatg agactatatt agaacttgaa gatgtcatag gtctttcaat
    12121 atccacaaga cctgactgtt tgccagatga tgttgtagaa tacttgtctg aactaaataa
    12181 aagaaccaat ttatgggttg aacttggact tcaaacaatt catgattcta catctaaaat
    12241 tataaataga ggtcatgatt ataaaacatt tgttgaaggt gttgaaaaat taaagtctaa
    12301 aaatataaag gttgtcgtac acattataaa tggtcttcct ggtgaagatt ataatatgat
    12361 gatggaaaca gcaaaggcag ttggtaaaat gggtgtagac ggtataaaaa tccatctatt
    12421 gcatgttata aaagatactc ctatggaaaa gatgttacaa aatggtatgt taactcttat
    12481 ggaacaagat gaatatatca atcttgtctg tgaccaatta gaaatacttc ctgaaactat
    12541 gataatccat agactcactg gtgatggtaa gagggatgaa ctagtaggtc ctatatggag
    12601 cctaaaaaaa tgggaaattt taaatcaaat tgatgatact ttaaaggcta gaaattctta
    12661 tcagggctgt aagtttgttt aatacaaaat aataactata gaaatggtta tataaatata
    12721 gtcctttcta tagttattta gtatttacaa aacaaaatat taatttgtac atttatacaa
    12781 tatataataa aaaatagata gtctaaactg tatctactta tattttattt gtactgtcca
    12841 ctttattcta taaaatttat aaatagttat tttttaatac caaatataat ttggtataat
    12901 ttttgttgga ctctaaaatg tataccaata aatccttttt aagtatataa ttctaaatac
    12961 tttcagtttt ttcaatctgc tttaacttgt tggacttaca aaatattctt atttcatcat
    13021 aagaaagatt aagtgctact actgcagata ttaacatcaa catcactcca aataacttcc
    13081 ctaaggaaaa actttctcct aatatagtgc aaccaattat tactgaacca actaattcta
    13141 ctgatgccac aacacctgca actgacaact ctacaccttt agatatacct gctgcataaa
    13201 aaatatatgc aagtgcagct ggaacaatgc ctaacattag catacaagat aaaacatcta
    13261 aatccttagc ataatttaag atttcccaag gtcttgaaga aggaatcata aatatagctc
    13321 caaataagaa actgtatact aatattgttg aacttgaaaa ttcctttaaa gcatttttac
    13381 taatagttgg cataagtgca tatgctattg ctgataatac tccaagtaaa agcccaaaag
    13441 cgtttaaacc ttgaaaatcc aaacgtccac cagtcactgc cataattgca ccaataaaac
    13501 aaagaataag tgaaaataat ttgcttctag taatattttc tttataacat aattttgaaa
    13561 atattgctaa aaacagtggt gatgtatata gtaaaactgc tgcaattgat acacccgcaa
    13621 tgtcaattgc tttaaaataa cataaattaa acattgcctg acatataatt cctattatta
    13681 ctgaatatat aattcctttt ttgtttatct ttaagatttg tggtgtcctt atagatgaat
    13741 atacgcctaa aactataaat ccaaaaaaca atcttgtaaa tgctacttgt tctgatgtca
    13801 gtccatttcc cattaaaaac tttccaaata atccaagtgt agcccataat aaccctgcta
    13861 ttgctataaa tacataccct ttactttttt ctcccatttt aaattccctt ctatttttat
    13921 ttagtcaatt tacactcttc tttttaagca aaaactatgc catcttaaat caatatatat
    13981 cgactctaaa accctagtct ttccaataat actaataaat tttcaatttt tttatattta
    14041 tttttcgctt actttaagtt gatttagact tattttaatt gtaaaatgag ttatatataa
    14101 cttatttaaa gttaaaatag actgaaactt at
//
LOCUS       DAMJYC010000100        14029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJYC010000100 DAMJYC010000000
VERSION     DAMJYC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.104"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            437..1330
                     /locus_tag="PC472_003174"
     CDS             437..1330
                     /locus_tag="PC472_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2728479.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1330..2724
                     /gene="gltA"
                     /locus_tag="PC472_003175"
     CDS             1330..2724
                     /gene="gltA"
                     /locus_tag="PC472_003175"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2728480.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIVF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3090..5309
                     /locus_tag="PC472_003176"
     CDS             3090..5309
                     /locus_tag="PC472_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728481.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNVKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5456..6202
                     /locus_tag="PC472_003177"
     CDS             5456..6202
                     /locus_tag="PC472_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728482.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6202..8082
                     /locus_tag="PC472_003178"
     CDS             6202..8082
                     /locus_tag="PC472_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728483.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8201..9592
                     /locus_tag="PC472_003179"
     CDS             8201..9592
                     /locus_tag="PC472_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728484.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9699..10739
                     /locus_tag="PC472_003180"
     CDS             9699..10739
                     /locus_tag="PC472_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2728485.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11228..11881
                     /locus_tag="PC472_003181"
     CDS             11228..11881
                     /locus_tag="PC472_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2728486.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11957..12166
                     /locus_tag="PC472_003182"
     CDS             11957..12166
                     /locus_tag="PC472_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728487.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12306..12872)
                     /locus_tag="PC472_003183"
     CDS             complement(12306..12872)
                     /locus_tag="PC472_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2728488.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13033..13686
                     /locus_tag="PC472_003184"
     CDS             13033..13686
                     /locus_tag="PC472_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2728489.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13844..>14029)
                     /locus_tag="PC472_003185"
     CDS             complement(13844..>14029)
                     /locus_tag="PC472_003185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728490.1"
                     /translation="DDFMYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVH
                     KFKLYVVNEKYIKGIME"
BASE COUNT         5494 a         1391 c         2373 g         4771 t
ORIGIN      
        1 ttatacaata tgaaagctgg tattgcaata gattaaagcc ttatacacgt ttagttttta
       61 aagaaattag tgttattatg aaaacctaag ttaatttatt aaataaataa ttgaataaaa
      121 aaattttatt atgtatttat aaaatttttg ttacaaattt ggcataatat agtatacttt
      181 aagtgtgtat taggttaaat ctataacaaa ttagacgtat gatatagact tagtgtttat
      241 tggcatttac aagaaatttg ttaatatcaa ctatttgtag tttgggcagt tataattatt
      301 atagggggga tgtccaatac aaaacaatca accaagaact aatattatat tataaagagt
      361 tatagttttg tgtgtttatg tttttaatta aatttaaagt gggaaaaata tatttgttag
      421 aatggagaat agcgagatga gtaataaaat agttagtaag agacaattaa cagatagtat
      481 ttatttaatg gaaatagagg cacctagagt tgcaaaatct tctcaaccag gtcaatttat
      541 aataattaaa aatgatgaaa aaggagagag gattcctctt acaatagctg actatgatag
      601 agaaaaagga acagtaacta ttgtattcca aacagttggt gcttcaacta aaaagttagc
      661 aatgtttgaa gaaaacgatt ttgtaatgga ttttgtaggg ccattaggac aagcaagtga
      721 atttatacat gaagatatag aagaattaag aaataaaaag atattatttg tagctggtgg
      781 agttggttct gctccagttt atccacaagt taaatggttt aaagagcatg gattagatgt
      841 agatgtaata attggtgcaa gaactaaaga attaattata ttagaagatg atatgaaaaa
      901 agtagctaaa aatgtatatg tatcaacaga tgatggtaca tatggattta atggaagagt
      961 tacagaccta ttaaaagatt tagttgataa tcaaggtaaa aaatacgacc aagcaatagt
     1021 aattggacca atgataatga tgaaatttat gtgccaatta actaaagaac taaatatacc
     1081 tactatagta agtttaaata caataatgat agatggaaca ggtatgtgtg gtggttgtag
     1141 agttagtgtt ggaaatgaaa ctaagtttgc atgtgtagat ggtcctgaat ttgatggtca
     1201 tttagttgat tttgatcaag caatgagaag acagtctatg tacaagactc aagagggaag
     1261 agctatgcta aaacttgaag aaggcgatag tcatcatcat agtaattgtg gatgtggagg
     1321 taacaaataa tggatgctaa aaaagtaaaa gtaccagtta gagaacaaga acctgctgtg
     1381 agagcaacaa attttgatga agtatgctta gggtataata aagaagaagc tatggcagaa
     1441 gctaatagat gtttagcttg taaaaaacca aaatgtgtgg gtggatgtcc agtaggaatc
     1501 gacataccag ggtttataac aaaaattaaa gaagatgata tagaaggtgc tgctaaagta
     1561 atagctaaga gcagttcatt accagctgtt tgtggtagag tatgcccaca agaaagtcaa
     1621 tgtgaaggtg tatgtatact tggaataaaa tcagatgctg tatcaatagg taaattagaa
     1681 agatttgtag cagattggtc aaaagaaaat gacataaatt tatctgatac agagcctaag
     1741 aaaaatcaaa aagtagcagt aataggaagt ggtccagcag gtctagcttg tgctggtgat
     1801 ttagctaaaa aaggctatga tgtaacaatc tttgaagcaa tgcatgagcc aggtggagtt
     1861 ttaacttacg gaatacctga gtttagactt ccaaaacaag ctgttgttca acctgaaata
     1921 gacaatataa gaaaattagg tgtaaaaata gaaactaatg taattgttgg taaaacaatt
     1981 acagtagatg aacttataga agatgaagga tttgaagcta tattcatagg ttcaggagct
     2041 ggtcttccaa tgtttatgaa tataccaggg gaaaatgcaa atggagtatt ttctgcaaat
     2101 gagtttttaa ctagagtaaa cttaatgaag gcttatagag atgattatga tacacctatt
     2161 agttctggta aaaaagttgc tgtagtaggt ggtggaaatg ttgctatgga cgctgctaga
     2221 actgcattaa gattaggttc agaatcatat atagtttata gaagaagtga aaaagagctt
     2281 ccagcaagag ctgaagaagt tcatcatgca aaagaagaag gcattgtatt taatacatta
     2341 acaaatccta aggaaatttt agtagatgaa aatggatatg ttaaaggtat ggtttgtata
     2401 agaatggaat taggagagcc agatgattct ggaagaagaa gacctataga aatagaaggt
     2461 tctgaatttg tacttgatgt agacacagtt ataatgtcac ttggaacaag cccaaatcca
     2521 ctaatatctt ctacaacaaa gagtcttgat ataaataaga agagatgctt aataacagat
     2581 gaaaatggtc agacttctaa agagggtgta tttgcaggtg gagatgctgt tactggagca
     2641 gcaacagtaa tatctgcaat gggtgctggt aaaacagcag cagcatcaat agatgaatat
     2701 ttaaaagcaa aagttaatgc ttaatttgta cattaaagag agggtgtctc aaaatgaact
     2761 ttttagttca tgaggcactc tttttgtaca aaatcaaata tgttttaatc atttcaacaa
     2821 tgaaaatttc attcctgtag aaagtctaac ttttaggtct tagtacaatc tctattttga
     2881 tgtagccact tttttagatg gtttaataat ttatcagtta ttattgtaat atttgatact
     2941 aagtaacata actttgtcta actatgataa aatagttata caataaaggt cttgtaagga
     3001 gtgaataatg tgtggctaat aaaatcagat ttgtcaaatc taacattaaa taatagaaaa
     3061 ataaattgtg taattaatgg tattttaata tgaaaaaaag agaaaaagta tttgatgggc
     3121 tagtttttaa gcatatatta atcttattaa tactattagt tttgattgat ttaacaagta
     3181 ccatcatata tgcaagtaac tctatggaaa ttacttctag aaatatgatt gaggcatcaa
     3241 aaagagaact tgaaaactat ttagaagtaa acatatcttt attaaaagct cttagtcaag
     3301 atgatagatt ttctaatggt gaaacatcac taattgaaaa gggaaaatta ttaagtccat
     3361 atcaaaagga atataatctt tttatgattg gtataacaga tacaaaagga aatgcttcaa
     3421 gtacatatag ggaaaatgtt aactcaataa aagataagcc tagttttgag aaagcaatta
     3481 aaacaaagca agtagtagtt tcagatattg aagtcagtaa tgtaacaggt gacaaagtat
     3541 ttatcatata tgtaccaatt gtaaaaaata atgagatgat tgggaccatt tttgcttctt
     3601 tttattttca agatgtaaat aatttaataa gtagaagcaa ttttgatgat tctattaaat
     3661 ttctaatgat agataaagat tatactatta tttctcatcc aaacaaaaaa tatgtaaatg
     3721 ataaaagtaa gatgcttgat ttagaaggga atattatagg aaccactaaa agtgaaatct
     3781 taaaaaatat aaataaaaaa tgtcaaggtg attttttatc ttgggataat tggagattat
     3841 ataatgtaaa atatacgaac gtaaagtgga ctaattggac tttagtatca aaatgtaata
     3901 tatttaagaa ctttaaaaat ctaatagtta attttatgat aaagttgtat ttttatatag
     3961 taatatttat gatattgtgg aaattaagta atgctaaatt aatagaacaa cttaaaaaat
     4021 tggcttatta tgattcttta agtggaataa aaaataaaga aaagtttaga aaagattcta
     4081 tgtatatttt aaagaattat tatcaagata atttttactt agtacaattg gatgtcaata
     4141 aatttaaata tataaatgag atgtttggat atgctgaagg gaataagata ttaattcata
     4201 tatcacaggt tttaaataat aatacaaata aatatgaaat ttgtgctagg atggataatg
     4261 accattttat attgttgatt gcttgtaaca ctgaagatga acttttaaat agattatcta
     4321 aaataaataa agaaatttgt aacttaaata ctactaactc atctaagtat aaaattgtta
     4381 tgtcaagtgg aatatataag attactaaaa aggatgatat taaaaagatt gacttgttaa
     4441 ttgatagagc aaatattgca gcaaaaagta aaaaggaaaa atatgaacat tcatattcat
     4501 tttttaatga agatacaaga aatcgattgt acaaggaaaa acgattggaa gataatatga
     4561 ataaagcttt agaaaaaggt gaatttatag tatattatca acctaagtat agtttagatg
     4621 atgtaaatga aattgaagga gcagaagcct tgataagatg gaatagtcca gaatttgggt
     4681 ttatttcacc tatagatttt gtacctttat ttgaaaagaa tggttttatt gtaaatattg
     4741 atatgttcgt gtttgaagaa gtatgcaaaa cattaaacaa gtggataaat aaagggtata
     4801 ctcctgtacc aatctctgta aatatgtcta gagttcattt atatagggat aactttatag
     4861 aaaatataac tgatttgata agtaaataca atatatctcc tgagtttatt gaacttgaac
     4921 taacagaaag tgttgtgttt gataatttaa atatattgat tgatataatg aaaaagatta
     4981 aagagatagg attccttata tcaatggatg attttggttc gggatattca tctttaaatc
     5041 tgttaaaaga cctttctttt gatattttaa aattagatag aggattttta atcgaaacaa
     5101 cagatacaaa acgtggaaaa attattattt ctaagattgt ggaaatggca aaagcgattg
     5161 atattaaagt aatctgtgag ggcgtagaaa cttatgaaca agtagagttc ttgaaagaga
     5221 taggttgtga taaggttcaa ggatacttat ttgctaaacc tatggtatta gatgaatttg
     5281 aaaaacactt aaactttaaa tttgattaat cattaataat tattagacta taagataccc
     5341 taacaactta cttacaaata tgtaagttaa ttgttagggt atttttatgg ttaaagtagc
     5401 atattcacga tattatggta ttgtagtaaa aataaaaaat tatggagaat ataaaatgaa
     5461 catattgagt attagaaata tatcaaaaac atattgtgga aatattccat ttaaagcatt
     5521 agataaggtt agtctaaata tagaaaaggg agaatttgtg tctgttatgg gaccatctgg
     5581 aagtggtaaa tctactcttt taaatataat atctacagtt gatagacaga gtgaaggtga
     5641 ggttgtatta gatggatatg atgtaagtaa attgaaagga gaaaaacttg ctgaatttag
     5701 aaggaaacaa cttggttttg tatttcagga ttttaatttg attgacactt taactgttgg
     5761 cgaaaatatt atgttgccac ttacactaga gggggcaagt atcaaagata tgaatataca
     5821 aactaaaagt atatctaaat ttttaggaat agataaaatt ttagatagaa aaacctatga
     5881 aatttcagga ggtcaagctc aaaggtgtgc tattgctaga gctattataa ataaacctgc
     5941 catacttctt gcagatgagc caactggaaa tcttgattca aaatctactg atgatgtttt
     6001 gaaattattt actaggataa acaaagaaca aaaggtaaca acacttatgg taactcatga
     6061 agcatatagt gcaagttatt cagatagagt aatttttata aaagatggtt gtatatatac
     6121 agaaataaaa aaatctgaaa gcagtaattc tttctattct gatatactag ctgttttgtc
     6181 tcaaatagga ggtgtaagat agtggatttt agaaagtttg cttataataa tatagttaga
     6241 aatttcaggg catatttagc atatttttta agttctgttt tttctataat gatatttttt
     6301 gtttttgcag tgtctatgtt ccatccaatt ataacaaaat ctggaatgca gagtggttct
     6361 actgcattta tggctcttat gacttcagaa ttaataatat tcggttttag tctacttttt
     6421 atattgtact ctttaggaaa tttcttaaaa tatagattga aagattttgg agtattgatg
     6481 ataattggaa tgagtaacag acaactgaaa aaattgatat taattgaaaa tttgataata
     6541 ggatttttag cagtgatgtt aggaatatta cttggtttat caatttcaaa attgttttta
     6601 ttgtatttaa gcaaactgtt ttatatgaat ttaactgaat tttattttcc agtaaaagca
     6661 atgctattga ctattgtatc atttatgatt ttatttttga taacaggacc aatgagctta
     6721 aaattattga gtaaaaagag cattttagag cttttagtag gtacaaaaaa gcctaaaaaa
     6781 gagataaaat cttcaaagct atttggaata attggtgtaa tatgtcttat attaggatat
     6841 gctatgatac tatttggcaa taagttaaaa ataaatggag gtatgtatac aacagcacta
     6901 ttaattactt tggggatatt tttatttttt tctcaattta ctattttgat attaaagtat
     6961 ttaaaaaata aagaaaaatt ttataagaac aaagtaaata tgttacttat aagtaatttg
     7021 atatataaat taaaggataa tacacgattg ttatttttaa taacaatact tttatcggct
     7081 actttagtag catttacaac aacttcaaca cttgtaagtt ctcaaggaga agcttctaaa
     7141 aataatttcc caatggttta ctcatatttt tcagagcata ataatgaaaa agaatatgaa
     7201 gaactacaac aaattagaga agcaatcaaa ggatatgatt ataaggagtt aagttttcct
     7261 gtacttaaat ataataggga agtcttacta agtgtaaaga attataacaa attatcaaag
     7321 gaattgggat tagataaaat tagttcaaag ttagaaaaat atgaagggat tataattcct
     7381 agtagcaatt cacaagaaca tataaattct ttaaaacaat taaaaaatta tagtataaaa
     7441 aatttaaata tagaagtaaa aaaatcaaaa aataaaataa tttttcctac aggactgttt
     7501 agtaaagttg tagttataag tgatgactta tacaatgaag taaaaggtga attttcaaaa
     7561 attaattttc atggatttga ttataaaaac tggcaaagca gtgcaaagat aactgaaaat
     7621 ttaaaagaaa aacattttaa tttggataga gaatataaca ggtcatattt tttaacatta
     7681 ccagatatgt atttagtaga gttacaacaa agtaaaattc ttcaatttat gggtgttttt
     7741 ataggagtga tattatttat agcagttttt agttttttat acttcagatt atatacagac
     7801 gagatagaag ataatgtaaa gtacaaaaaa ctatcaaaaa taggtctatc ttttaaaaat
     7861 atgaataaga ttgttagtat agagataggt acattgtttt ttataccgta ttttatagca
     7921 attataaata gcataatttc tttactgttt ttaaatcaag tattaaataa tagttttaag
     7981 ttagaaaatt tgggtatatt actactgata tcatctatat attcacttta tttttactta
     8041 ttaaaaaaaa tttatactag aaaagtttgg ttttttgatt aaagtggtat ataatagata
     8101 tataaattaa tatttaaaaa gtagttaata ttaggggagg gcatttgcac ctcccttgta
     8161 atatgtggaa aattgaatat tagaagaagg aggagacttg atggaaaatc aacaattatt
     8221 aggaacagaa cgaattagta aattactact aaaatattct ataccagcta taataggtat
     8281 gttggtaaat agtttatata atgtagtaga tagaattttt attggaaata taccaggggt
     8341 tggaccactt gcaataacag gtcttggtgt aacaatgcca ataatgacaa taatacttgc
     8401 atttgggatg ttgataggta taggaactac tactaccata tcaataaaac ttgggcaagg
     8461 taaagtagaa gaggcaagaa aacttatagg aaatgcaatg acgttatctg taataacagg
     8521 aattatcata atgatacttg gaatattatt tgcaaataaa atacttacat tgtttggagc
     8581 aagtgaaaat actttaatat atgcaaaatc atatatcaat attatattat taggtacagt
     8641 agtaaatctt ctttcctttt ctttaaacca ctcaataaga gctgatggta gtcctaaaat
     8701 ttcagcagga ataatgatag taggatgttt aactaatata gttttagact ggatattaat
     8761 atttggattc aatttgggaa tacaaggagc tgcaattgct acagtaactt ctcaggctct
     8821 tacagctatt ttaactatag ggtattatat aagtggaaaa tcaaacttac gattcagtaa
     8881 atctaatcta aaattagata aaaagctaat aaaagcagtg tttgcaatag gtatgtcgcc
     8941 atttgctatg caacttgcag caagtttagt tcaagtaata tcaaatattg cacttaaaac
     9001 tcatggtgga gatttagcta taggggctat ggctactatt tcttctattg caatggtatt
     9061 tttgatgcct atctttggaa taaatcaagg agcgcaacct attataggtt tcaattatgg
     9121 agctgaaaaa tatgataggg ttaaaaaagc atatttaggt tcattagtag tagctactat
     9181 aattttatgt atgggtatgg tagtagttat gcttttccca gaagctatta taggtatatt
     9241 taataaagac cctgaactta tgaatatatc tgttaatgga ttaagaatat acttacttat
     9301 gttgccaatt gtaggcttat cagttacagg aacaaacttt atacaatcta taggaaaagc
     9361 aaaaatggct atgttattga gccttttaag acaagtaatt ttattgatac cagcagtatt
     9421 aattcttcct acattcttag gtttacaggg tgtatggaca gctcaaccag tgtctgattt
     9481 tatagctaca gtaattacag gtatagttgt atttagagaa ttaaaaagat atactcctaa
     9541 aactgaaaaa ttaaatgaaa atgaaagact aaacgagata actactgagt aaaaatataa
     9601 attcagataa ttgagcaaga aattgcaaaa aatacgatta tatgataaaa taaaaccgct
     9661 gtcaaggata agagaaattc taattatgga gtgattttat ggaatttaca aagaaaagca
     9721 aattaaaaaa tatgtatgta aacatgaatg tagctagaat gttgtctaag ataaatgagt
     9781 ataagggtag acaactgctt tacaaaaaac aaccaaaaga aattttagaa aacttagaaa
     9841 aaaaatcttt agtagattgt tctgaatcta catacattgg aaatcaaaga gatagtagta
     9901 attttaatct agaaaagttg ataagtaatg aagttacacc tagaagtaga gaggaattga
     9961 gtattgttga atacagagat gtggtaaaaa ctattaacag tgcttatgag agtattccca
    10021 tcagttcaca aacgattctt gagcttcatg gatacttata taaattttca tctactagag
    10081 gaggaagtta taagtcagat aatgatttta ttgaacacaa tttaaaacct agcgaattta
    10141 taagtactga ttcaagtgtc aataaagttg aaaaagctgt agaagaaata tgtgaagctt
    10201 ataatacatt gatagaggaa gatgaaattg atatattaat attaatttct gcatttgtgt
    10261 tagatttcat attgatacat ccattcaaag aaggtaatat aaaaatggcg agagtactta
    10321 tcctattgtt actaaataaa aatgggtatg aagttggaag atatataagt ttaggaaaga
    10381 tatttgatga tagttcatat gaatattata gtaatttaaa ttatttgaaa gcctcaatag
    10441 gtagtgaaaa agcagatatg aatgcatgga ttgagtattt tttagagacg atattaactg
    10501 catatgagaa gctggatgat agtttaaata tatctgataa gaaaagacaa acaaaaacta
    10561 gtagaataga gaaaataata aattctaccc ttggatattt tacaaaagaa gatataagag
    10621 atttatgtcc tgacattcca gaacctacta taaatagagt ttttaataat ctaagaaaac
    10681 aagataagat agaagttgtt gcaagaggaa gaagtgcaaa atggaagaaa aaatattaaa
    10741 aaatgtttag ataactaaaa tggtatagtg ttttacacta taccattttt aatgtcatta
    10801 gcatgagcaa taatttaaca acttaagcaa tatcaatttt atttattgat tatttttgaa
    10861 ctgaaaaatg gcgttatatg gtatacctca gtggtaatta attttggatg agaagatata
    10921 atattacaga atatattagg agtatatgta gaaaaaatat aataaaagtg tgaggcttag
    10981 taattaacaa attatgtggc ttcacacttt tttataaaat aaagatactt agcagaataa
    11041 tattttagtt gatatgttta taagatggta cttgtttggg taaaagtata aatagtaaaa
    11101 aagtagaagt aatgtgtata aaaacataag ttacattctg taataatagt tattttataa
    11161 ttcaacaaaa gaatagaatg ggtatatttg tgaaataata atataaaata atcttaggag
    11221 tgagcttatg aagaaaaaat tattatatat aaatgtaaat tcaaaacctg aagacttatc
    11281 ttcaagtaaa actgtagcaa gaaaatttat aaataaattt atggaaaaaa acaaagattt
    11341 tgaggttgaa gagatagacc tttataaaga acatatacca agacttgagt atcagtactt
    11401 tgaaaaaaga aatagcatag ttagtgaaga aaatgctaaa aatttagacg ataaggatag
    11461 aaaagagctt acaaagatta gaaatctttg tgaccaattt gtgagtgcaa gtgtatatgt
    11521 aatagcagca cctatgtgga gtttatcttt tccagcacca cttaaagagt atatagattg
    11581 tataattcaa gatggaaaaa ctatatcttt tgaaggcaat gataaaccac aaggaatttt
    11641 aaatgatatt gatagaagta tggtatatat acaatcttct ggtggacaca taccatgggt
    11701 gttgaagcca gttatgaata aagggttaaa ttatgtggaa agtattatga aatttatagg
    11761 aattaaaaaa tttgatgaat tgttagtaga tggaactgga actagtgaag aagaaagaca
    11821 agctgcaata gaaaaagcat ctgaaaaaat agatggtata atagatggga tgaaatttta
    11881 attataatat tttattaaaa atataaagga aaaacctctt ttaatgtaga aacttatatt
    11941 gaaggaggcg agaatcatgc gagaagaaaa aagtaatgaa aagtatgatt gttattggtg
    12001 taatcaagag aataatttct gtgtagaaat aaaagataat atagtcatga tagatgatgg
    12061 cactggtacg ttaaaacaag ccgttttcat agggtataaa caaatccaaa ttaatttaaa
    12121 ttgttcacat tgtcaaaact taaatagaat aaaattaaat ttgtagaatt ttaaaatgga
    12181 aattattgaa ggaattaaaa atagaaagtg ttaatattta gactgaaaat tatattttat
    12241 acaataaaag tttatttaaa taacattgta ttttaatatt tattttcagt cttactttat
    12301 taaaattatt ttttggttat gtctggaaaa tatctgtcta ctaatttttc tgaaatattt
    12361 tttaattggt cagccattga agtcagactt aataaggcta tcaataaatc atctttattt
    12421 tctggattta tactaatatt ttcaaaatta gactctataa agtccggtat attctttctt
    12481 tgattttctt ttattaacat gaatttttcg taacatgaaa ctaaatcttt ttcactgaaa
    12541 ttatcctttt catacacacc atgtaagatt cttttaattt cttgtattgt aagtgtattt
    12601 ttcatagaat aaataattat catctgtaaa atttgttctt tagtatactt ttttccctta
    12661 actggtttga ttaatccctc tttgctgtaa ttatttatca tggttttagt aagaatttta
    12721 tcactttcaa atcttttatt agcagaaagc ttattgtcaa ttagagaaat aatttgatcc
    12781 atatataagt caatagatgg aatatcgttt gaagttatat ctgcattatt tagtgtttct
    12841 agtattatag tattaagttc attattcatc atatgttacc tccataatat atataataca
    12901 ctaaattaga aagtaaaaca atattaaggc ttgtaaaaaa aacataaagt ggtataatta
    12961 aatatataat atagtaatgg aaactatatt tatgaatctg atttttaaaa tacataattg
    13021 taggaggacg aaatgtatca atttttttta aagggtagag acccaataag cagtttaact
    13081 cattttatag gtgcttgttt atctctttta gcaactataa ttttagtttt tcaatctgtt
    13141 acattacagg aaacttctct cttgatgata gtatctgtat cggtatttgg actttcacta
    13201 attgctttat atagtgcaag ttcgtattat cattttttaa aggggacacc agaacaagaa
    13261 ttgtttttta gaaaagtaga ccatgcaatg atttacgtat tgattgctgg ctcttataca
    13321 ccaatatgtc tgaattttat ggaaaaaaaa gaaggcataa tatttgttac agcaatatgg
    13381 atagttgcat ttattggtat aataataaaa atattttgga tggatgcacc tagatggtta
    13441 tctaccagta tatatttact tatgggatgg gcaattgttt ttgatataaa tgcattcaat
    13501 tcaattccta aggattgcct tagactatta attatggaag gtgtatctta ctctataggt
    13561 gcaattattt atataattaa aaagcctaat ataagtcctg agtttggatt tcatgaaata
    13621 ttccatattt ttattatgat aggttcttta tttcactttt tagcagtttt attgtatgtt
    13681 ttatagtttt aaaagatttg attaattgat actaaaattc tacaatgaat aaaatggaac
    13741 tgccttatat agattatatc ttcgacaata agacagttcc ttattataaa tattaaattc
    13801 tacttataat aaaattttga taaaaatagt ccattcaaaa tgcctattcc ataattcctt
    13861 taatgtactt ttcatttacg acatatagtt tgaatttatg tactataatt atatcctcta
    13921 aattctctga aacactaaca attctgacac atttgtttct aatttctaca ggtaaatcta
    13981 aatcatttgt taaattcaca tagtcatcat atttatacat aaaatcatc
//
LOCUS       DAMJYC010000101        13962 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJYC010000101 DAMJYC010000000
VERSION     DAMJYC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.105"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..439
                     /locus_tag="PC472_003186"
     CDS             <1..439
                     /locus_tag="PC472_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2728491.1"
                     /translation="RDLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIVAKLIAEG
                     ILNKTIDNKENGEGKIMYKHTIVYDGAVDKIPATVVGWGYNDGKILICDIKDYIPGQT
                     ENLYVVGGGACEKIGSMTKENYTMIKGNDRFDTLYKALDFINK"
     gene            complement(485..670)
                     /locus_tag="PC472_003187"
     CDS             complement(485..670)
                     /locus_tag="PC472_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2728492.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            1395..2072
                     /locus_tag="PC472_003188"
     CDS             1395..2072
                     /locus_tag="PC472_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2728493.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            2241..4277
                     /locus_tag="PC472_003189"
     CDS             2241..4277
                     /locus_tag="PC472_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2728494.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(4468..5034)
                     /locus_tag="PC472_003190"
     CDS             complement(4468..5034)
                     /locus_tag="PC472_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2728495.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            5322..6257
                     /locus_tag="PC472_003191"
     CDS             5322..6257
                     /locus_tag="PC472_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2728496.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            6722..7936
                     /locus_tag="PC472_003192"
     CDS             6722..7936
                     /locus_tag="PC472_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728497.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLNQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(8356..8877)
                     /locus_tag="PC472_003193"
     CDS             complement(8356..8877)
                     /locus_tag="PC472_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728498.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            9233..10030
                     /locus_tag="PC472_003194"
     CDS             9233..10030
                     /locus_tag="PC472_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2728499.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            10140..10730
                     /locus_tag="PC472_003195"
     CDS             10140..10730
                     /locus_tag="PC472_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2728500.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEILNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            10796..12493
                     /locus_tag="PC472_003196"
     CDS             10796..12493
                     /locus_tag="PC472_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2728501.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            12707..13546
                     /locus_tag="PC472_003197"
     CDS             12707..13546
                     /locus_tag="PC472_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2728502.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         5508 a         1456 c         2258 g         4740 t
ORIGIN      
        1 aagagatctt tactggctta gtcatacaaa agctcctgca atattaatag aagtgtgctt
       61 cgtagatagt aaagcagata cagattatta tattagacat aaagacatag ttgctaaatt
      121 aatagcagaa ggtattttaa ataagacaat agataataaa gagaatggtg agggaaaaat
      181 catgtacaaa catacaatcg tttatgatgg agcagttgac aaaatacctg caactgtagt
      241 tggctgggga tataatgatg gtaaaatact aatatgtgat ataaaagatt acataccagg
      301 tcagacagaa aatttatatg ttgttggtgg tggagcatgt gagaagatag gttccatgac
      361 taaagaaaat tataccatga taaaaggtaa tgatagattt gacactcttt ataaggcatt
      421 agattttata aacaagtaaa tcgattagga ggtagtaact ggaaatagtt actacctctt
      481 ttttttattc attttcagtt ttttcaataa ttatttttcc atcctcaaaa gtaacaagaa
      541 catccctttc atcaggagaa atattcatct ctttaatcca tgacattgga agtatcaatc
      601 taggtgtaaa agaacctttg ccacttttat tgaatgcaac tctaagtttt ctttgttcca
      661 ataatatcat tcctttctac tattaataaa tgccaacaat aataaagtaa ataccaaagc
      721 aataagagct ttaaaagtaa aacttattat tatattaata atatttaaaa ctatagtgat
      781 accaagaata atcatacaaa ttctataaaa tttattattc atattaaatt agaaaatgtg
      841 ttataatatt agaaagaagc tacctagaag gaaggtattt tccttctagg attgaaactt
      901 ataattcttt aatcaatctt agtattgcga gtactagatt aattaaaact aatgtgaaag
      961 agaaagcatt tttaatttta ttaaatttgt tttctctttt attttttctt ctttctaatc
     1021 tccccacttt ctttgtcctc ccttctaagt tttttatttt cttacctcct ttctatattt
     1081 atattatatc atcacgtgac gtgaaagtca ataaatattc catatttttc aataaactta
     1141 tattgataaa ctatattttt tacaaataaa aataaatggt gacaaaacca aatttgccac
     1201 cattttgcca ccgaaaataa ataatagaat aaatttaaat taaacataat aaaacataaa
     1261 caacaaaact gaccttaata aaacattgaa gcaccaacac ttgtaaaaaa taacaacaat
     1321 aaacaaataa cctagaataa tattacaact taagtaaata ataattttga ataataattt
     1381 acggaggtat tcatatgttt aaacacgata aagcattatt aaaagaagta aaagtagaaa
     1441 gaccaaatcc tcaatatgct gttttaatgc aagaacagtt gggtggtgca aatggagagt
     1501 taaaagcagc tatgcaatac ttgtcccaaa gttttagaat aaaagaccct caaataaaag
     1561 atttatttct tgatatagca gcagaagagc ttagccatat ggaaatggta gcacaaacta
     1621 taaatttatt aaatggacat gatgttgatt ataatgcagt taatacaggc gaaatagaaa
     1681 cacatgtatt aactggtcta tcaccagtct taataaactc atcaggagct ccttggactg
     1741 ctaattacgt tacagttaca ggggatttag tagctgactt actttcaaat atagcttctg
     1801 aacaaagagc taaggtagtt tatgagtact tgtatagaca aatagatgat aaatatgtaa
     1861 aagaaacaat agatttctta cttaatagag aagaagcaca taatgctcta tttagagatg
     1921 ctttaaataa agttaaagat acaggttcaa atagagattt tggagttaca gaagattcta
     1981 aattatattt cgatttgtct actccagggc caaatcacga tactaagata gatattaatc
     2041 ctccttcttt tgaaaaacct ttaaaaaaat aatttgatat tttttatttt gttttagtag
     2101 ttataaaaat tatttactag aagaattaat tatatgaaaa attaaatatg tatagttaaa
     2161 aatgggtata cacttattat aagtgtatat ctatttgttt attgcaaaac atataataaa
     2221 ataacaaatg aggtggtatt ttggacagta atagaaaacc taataatcta ataaatgaaa
     2281 aatctccata tcttttacag catgcttata atcccataaa ctggtatagt tggaatgatg
     2341 aggcttttaa aaaagcaaaa gaagaggata aaccaatatt tttaagtgta ggatattcaa
     2401 catgtcattg gtgccatgtt atggaaaaag aatcttttga agatgaagaa gttgctgaaa
     2461 taatgaatag aaactttgtg gcaataaaag tagataaaga ggaaagacct gatgtagata
     2521 gtgtgtatat gacagtttgc caagctatga caggtagtgg tggatggcca atgaccataa
     2581 taatgacacc agataaaaaa cctttttttg cgggaacata tttcccaaaa tattcaaggt
     2641 ataatagacc tggagttata gatttattag agaatgtatc tgaaaagtgg aatacaagca
     2701 gagatatatt aataaaatct ggagatgaaa taatagaagc tcttaaggat gattttggtg
     2761 taaaaaatac tgagggtgat ttatcaaaag agatgttgag ttcatcagtt agagtattta
     2821 aggctattta tgatgaaaag tatggtggtt ttggtaatgc tcctaaattt ccaagtcctc
     2881 agaatcttat gtttttaatg aaatattata gtatagaaaa agacaaagat gtgttaaaaa
     2941 tggtggaaaa aactttagat ggaatgtata gaggaggctt gtttgatcat attggatttg
     3001 ggttttcaag atactctact gataaaaagt ggttggctcc tcattttgaa aaaatgctat
     3061 atgataatgc tatgcttact atagcttttt tagatgcgta taaaataaca aagaaagagt
     3121 tgtataaaga aattgctata aaaactatag attatgtagt tagagagatg aaggataaag
     3181 aaggtgggtt ttattcagca caggatgctg atagtgaagg tgaagaaggt aaattttata
     3241 catttaatcc tcttgagata attgaagttt tgggtgaaga agatggaact ttctttaata
     3301 attattttga tataactagt agtggaaatt ttgaaggcaa aagtattcct aatcttataa
     3361 aaaataaaga atatgagaga cataatgaaa agatagctga ccttagtaaa aaggtatttg
     3421 aatatagaaa agaaagaaca tctttgcata aagatgacaa gatacttaca tcatggaatg
     3481 cattgatgat agttgcttta acaaaggcat atagtacttt aaaaaatgac atatatttag
     3541 agtattcaaa taagtgtctt aactttataa ataataatct tgtaaatgag tcaggtagat
     3601 tattagctag atatagagat ggaagttccg actatttagc atatttagat gattatgctt
     3661 ttttaatctg ggcgtatata gagctttatg agtcaacatt taatatgaaa tatttagaaa
     3721 aggctttaaa tttgaatgaa agctgtatca accttttttg ggattatgaa aaaagtggat
     3781 tttatatata tggtaaagat agtgaaaatt taatagcaag accaaaagat ttgtatgatg
     3841 gagctattcc atcaggaaat tctgttcaat tatacaatct tataagactt gcgaaaatta
     3901 ctggagataa tagacttgaa gaaatgtcat ataaacaatt aaaactgtat gtggataatg
     3961 taaagagttc tccaactggc tacagctttt atatgctttc attgatgttt gaactttatt
     4021 ctactaagga aattatatgt atttttaagg aagattctga tttaattgca tttaaagaat
     4081 tgataagtga aaattttata ccaaatgcaa cttttttagc aaagaaatac aatgaagaaa
     4141 acactattat aggtttttta aataactata tattgaagga tgataagact agttattacg
     4201 tgtgtcaaag taatagttgt agtcaaccaa ttaatgattt acaaaaatta aaagatatga
     4261 ttttaggagt agaataatca taatagcgaa aaaggagatg ctttaaaata aaagtctaat
     4321 catctccttt ttttgtataa aaaacagaaa aagagttcct aagaacacct aatctatgta
     4381 atgattgttt tgttaaaaaa taaaaatgac attatagtta tataaaatat tgtactataa
     4441 aaaagtggta ttatagatgc gatagtatta atctttttta tttactctat ttcttctttt
     4501 tgcaaattct tcaagtgctt cagatttaat tttatctatt tcttcatatt ccataaaatt
     4561 agcatcttct tcaaagccat cttgttcttc accattaaaa cgatacattt tttttagtaa
     4621 actccataag gtctctaatt catctttggt aaattcgtga aatacatctg ccataaagtt
     4681 tattccagtc ctactacatg tcaccataac cttttttcct aagtctgtaa ctttaacatt
     4741 aattgctctc ttatcatgag gacttggaat tacatcaaca tatccatttt tttcaagatt
     4801 agcaactagt ctatttatat tttgtttact tgtacccatt tttcttgcaa tattatttaa
     4861 tgttgtttct tcctctggta aatgaagaat tgataaaata gtcatatact gtcttgaagt
     4921 aagtatacca aaatatttat ctccttctat ttgtattttg tttgtaaggg taaaaagagt
     4981 tgcatatgtt tgattcatta aaaagagctc ttttagttca tttgaataat ccatgaaata
     5041 tatctccttt atttttagat gttgttttgt cgaaaacaga atatcatgaa ttaattctaa
     5101 tgtcaatatg gtgtcaaaat atattaaagt attacttttt tatgtaacta ttaattaatt
     5161 aaattattgt ataattttat gtaaaaactt ctatgtacca atgataactt ctatgaggaa
     5221 ttaataattc ttaaattttt tagaaagatt ttgacttgaa aatacttgta atataatata
     5281 taaattaata tactgaataa aatcaatctg gagggataat tatgaagtgt acaaacttta
     5341 ctttcaaagg agaggaaggt ttagatatat atacatataa atgggaagat gaaaatataa
     5401 aaaaaccaaa ggcagttatc caaattgcac atggaatggc tgaaactgca caaaggtatg
     5461 agacttttgc caaggtactt actaaaaatg gttatatagt atatatcaat gaccatagag
     5521 gtcatggaaa aactgcaaag ataatagaaa atgttggaca tttagctgaa aaagaaggat
     5581 ttagatgtct tgtagaggac atgtatactt taacaaatat tataaagaaa gaaaatgaag
     5641 atttgccaat ttatctattt ggacacagca tgggttcatt tgcaagtcaa agatatataa
     5701 tggattacag taataattta tctggtctta tactatgtgg ttcaaatgga aagcaaggaa
     5761 tcattctaaa tcttgctcat ctgataatca atcgtgagat taaaaagtat ggaagacgtt
     5821 ctaaaagtaa taaaataaat aatttgatat ttggcggcga aataataaga agaaatgaaa
     5881 agactaagtt tgactggtta agtagagata aagaacaagt tgaaaaatat ataaatgacc
     5941 cattttgtgg agtagtgtgt agttgtggct ttttttatga tttagttcaa gggttaaaag
     6001 aaattgagga taaagaaaac ttaaaaaaag tgccacttga tatacctatt tacataatat
     6061 ctggagataa agaccccata ggaaaaaatg gtaaaggagt cttaagacta agggatagat
     6121 atataaaatt aggagtaaaa gatgtaactt gcaaacttta caaagatgga aggcatgaat
     6181 tgttgaatga aataaataga gaagaagttt ttgaggatat aatttgttgg ttaaataata
     6241 aaatagaaat attgtaataa aaaaatataa tgatatattt actttaaatt tatttatata
     6301 taatatttga aaagtggatt atgtatatgc aaagtagaaa agagagagcc cagaagtgat
     6361 tttttgctat aatgccattt attgaatagg tgggtgttat tataaataaa ttattaacac
     6421 tcagtattat tatcattgta tatggaattt attttattta aaattccttg aaagtttaaa
     6481 tatagtaagt tgtagtaaag agaaaatata aatatttaaa tgctatttaa tgtgaatcta
     6541 ttaattaggt tatacttaaa cttaattaat agattttata tttcaagaca atgagatagg
     6601 gttttagatt aaaatataac caatggttaa attacagaca ttttgttaac tttccctcga
     6661 tttactagta aatatttttt ttatatactt aaattacaac agatgtacta gggaggtata
     6721 tatgcaaatt aaaataggta aagtcataca gtgtcttaga aaagaacgaa atttaactca
     6781 agaacaatta gcaaagttta taggtgtgtc aacaccagca gtatctaagt gggagagtgg
     6841 aaattcttac ccagatatag aattattgcc tttattagca gactttttta atgtgtctat
     6901 tgataagctt ttaaattaca agattgattt aagtgaagaa gaagtaatga aaatttataa
     6961 agaattagaa tcaggctttg ctagaattga aattgattta tcaacagaag aaccaaaaga
     7021 agaatttaga caagacttag aatctgtaaa aaagctttcc aatatgtata tagagaagta
     7081 tcctaaaagc tatttattga agctaaggat atgttcgctg tatcagatgt attcatataa
     7141 atttggtaag agtgaactta atgacagagt aaaagaaaca acgaatattc ttgaagatat
     7201 agtgagaaat acagatgata tccaaataaa agaaacagct cttattatat tatcaaacgc
     7261 atattgtatg ttggaggatt atgaaaaggc agagttgtat ttaaatatga tacataagtc
     7321 aataggggat acaagtgtta atttagctat gatatattta aatcaaaata gacttgaaga
     7381 agcagaaata ttgcttcaaa ataaattgtt tagtaatgta tttaatataa gcatggattg
     7441 taagggaata attaatgtat acaaaaatca atacaaggaa ttaaagaaaa aattggagaa
     7501 tagaaatttt aataaaaatg caattgagag agaaatggag tatataaaaa ataagttgct
     7561 ggggtatgca aatctttctt tagaaataaa aaaaatgctc agtgaagata aaggtgcatt
     7621 ttttagtatg tatatggatt atatggaatt atctctaatt tttttatttt ttaacatgaa
     7681 agaggaagca aaaaaagcct tatatagtct aaaagaaata ttagaaaaat atcctataca
     7741 tgaaaatttg gatgtaagtc aaatgagatt ttttgataag gtagagtcta aaaatttata
     7801 tacttttaat atatatacaa acttgttaat tgtacttaat gatgatagtt ataatgagct
     7861 tagagaagaa ccaattttta aggatgtaat tgaaaagatt ttagatatgg aaaaaatgtt
     7921 gaaaaataaa gaataagtaa aaatttaata taggacttaa agattaatca aattaaaaga
     7981 aagtgtttca aaataaaaat ttgaatcacc ttttttgtcg gtcttaaaca gttatttaga
     8041 aaaatagagg tttcaaaata gaaatctgaa ttatctgtat tattacacaa aaatagaaac
     8101 ctaaatttat aaaaatagaa taaagtttgt tatataaaat gtaattttgt ctataaaatc
     8161 tcacttgttt tcttaacttt aagcgtgaaa aagagaatgt atcatgaatt ttttagttca
     8221 tgatgcatcc ttctttgtat gaaatccaat atatctttat tatttaaaca atggaaaaga
     8281 gccttatttc tattttgaga catctctttt atttaattat gaagttttat acagttcaat
     8341 aaataggaat tatatttaga aactcatgac attatttctt agatagtgat taaatttatc
     8401 atattccatt ttagataact tttctgggta agacattaca atcaggttat gcccattaaa
     8461 tttttcatgg tatggtggat gaatgtgttc ataattattt gtcttatatt ttactctttc
     8521 atagaacgcc ttgcgataaa ttgaaatctc atcaattgga aggtcaattt ctaaaataat
     8581 agtttttcct ttcttattta aaagctctaa cgctctttgc ccatatctat tattgcgcat
     8641 ttctggaaaa atgcagaaat gttctacata gataaagtca tctgtttccc aacataaaat
     8701 tatgccgaca aactggtttt tatcataaat taaattgaat tgatattctt tatttttcaa
     8761 aatttctgct tgcaaagaag attttctttg ttcatgaaat gggaaactaa ttttatataa
     8821 ttccatagcc ttgtaatata atttattttc actggttctt aaccgttcaa atttcacttt
     8881 actcctccta atcacgaaaa ttccaattta tttatgagtt cattttacca tagtcagtat
     8941 ttgcatacaa tgtgttttaa gttgagtgtc atttctttct tttatttatg cttatatctc
     9001 atagtctaag agataattgc ttaagacata actaaaaact attatattaa gctttgataa
     9061 tctaaaaaca gtatatttat ggacttttct aggtctaaaa gtaatcttaa taattttaga
     9121 agttacaaaa aagttacata aatttggaaa gctataatat acatatataa tataaggtta
     9181 aaactgtaat gaataatatt aatttaaaat ttgaacatgg aaggagatta ttatggagaa
     9241 aagagaaaag aggataataa tatcatcttt attaagtgta tcaattttaa tgggtttggc
     9301 aagtatatat tctatattaa ataaagaaga cataagttta accgttaaag gtcaagagca
     9361 aaaagtatct tcctttaaga aaacagttga ggaacttttg gatgaacagg gtgtaaagta
     9421 taattctgaa gataagatta atccaagttt agatacagaa ctaaaagatg atatgaaaat
     9481 aaaagttgtt aaggtaacta aaagtaaaaa agaagagatt gaaaaaattc catttgatac
     9541 aaagcatgta aatgatagta atttgttaaa aggaaaatct aaagtttatc aagaaggtca
     9601 agaaggagaa aaaaaactag tctataattt aacttaccat gatggaaagt tagtcaaaaa
     9661 agtcttatca aaagaagtaa tatctaagga gccaactaca aaaattataa aatatggaac
     9721 taaagaaaaa gtattaatag catcaagagg agcaaatata agaggaggca aacatatgaa
     9781 agtggttgcc actgcatatg caggagatac aataacatct actggtacaa ctccaagatg
     9841 gggtgttatt gcagttgacc cacgtgtaat accatatgga acaaaagtat atatacctaa
     9901 actgggtatg acttttgtag cagaagattg tggaggtgca attaaaggta atagaataga
     9961 catttttatg aacagtgaag gaaaagcttc aaattgggga aggaaaagta tagatatata
    10021 tctacattaa attagtgaat agaataactt tacttgaagg acaaaaaagt ataaaatatg
    10081 aattaactaa actgttagat tattcttaaa acataaaaat atatctaagg ggggataaga
    10141 tgaaaatagt ctgcttagga gacagtttaa cgtatggatt tggagtatca cgaagtaatt
    10201 catggacaaa cattgtaaat aaagagactc gattagaaat attaaataag ggtataaatg
    10261 gtgatacaac aagtggtatg ttagctaggt ttaatgaaga tgttgtaaaa aactctcctg
    10321 atatagtttt tataatgggt ggtaccaatg attttattgc tggtgcggga aatgaaatta
    10381 ttaattctaa tataatggct atggtgcatc agtcatattc aaaaaatatt atacctataa
    10441 taggtatacc attaaaacca gatattccaa atgttaggga agattggagt tgttttactg
    10501 attttaatat agtatctcaa aacttagaat catatagtta ttggataaaa aaattttgta
    10561 tgacatttaa tactaatttc gtagattttt attcagaata taataaaaat atggaactag
    10621 aaggatataa aaaattatat tttgatgggt tacatccaac caaagagggt catagaataa
    10681 tggcagacat ttttattaat tcaattaata agtatataga agagttatag attacattaa
    10741 aatgcttgtt tatgcatcag ttgcaataaa tacatacagt agagaggggt ttattatgga
    10801 ttatactaaa acttatgaag agtggataaa gggttcatat tttgatgaag atacaaagct
    10861 agagttggag aatataaaaa ataatgaaaa agaaatagag gatagattct ataaggattt
    10921 agaatttggg actgcgggtc ttagaggaat aattgaagca ggaacgaata gaataaataa
    10981 gtacacagtt agaagagcta cttttggatt ggcaaactat atattagaaa acacaacaaa
    11041 agaagaaaca agtagaggag tagtaatagc tcatgataat agacacaagt ctagacaatt
    11101 ctgtatagaa tctgcaaata ctttggcagc atgtggtata aaagcatata tatttgatag
    11161 tttgagaact actccagaat tatcttttgc tgttagaagc ttaaatgcaa tcgctggtat
    11221 agtcataaca gcaagccata atcctccaga atataatgga tataaggtat actgggaaga
    11281 tggagcacaa gttatgccag agattgcaaa tgcaataaca gagaaagtaa atagtataca
    11341 tgattatagt acaataccaa ccttaacgga agctaataaa aatttggtag ttttgttgga
    11401 cgaatcacaa gatactaaat ttatagaagc agtaaaaagc caaattataa gaaaagattt
    11461 agttaaaaat gtaggtaaaa gctttaagat agtttataca cctctttgtg gaacagggaa
    11521 tgttccaata agaagagcat tgaaagaagt tggttttgaa aatataattg tagttccaga
    11581 agaagaaaat ccagacccaa attttgcagg gcttgattat ccaaatccag aagagaaaaa
    11641 agctttaaat agaggtatat tactcgcaaa agaaaaagga gcagaccttg taatagcaac
    11701 tgacccagat tgtgatagag taggtgtagc agtaaaaact actacaggtg agtatgcttt
    11761 acttacagga aatcaaattg gtggtatgct gacacattat ataatagaag gattaaaaga
    11821 aaataataaa ttaaaagaaa atcctacaat aataaaaacc atagttacat cagaatttgg
    11881 agcagatata gctaaagcaa ataatgttga tgtattaaat gttcttacag gttttaaatt
    11941 tattggtgaa aaaataaaat tatttgaaca aaataaaaat agaagttatg tatttggata
    12001 tgaagaaagt tatggttact tagttgggac tcatgctaga gataaagatg gtgtagtttc
    12061 ttcattgtta atatctgaaa tggcagcatt ttactattca aaaggtatga gtttatatga
    12121 tggtttaata gaactttata aaaagtatgg atttttcaaa gaacagacta tatctttgac
    12181 tttaaaaggt atagaaggtg tagaaaagat aaaagaaata atatcttatt ttagagaaaa
    12241 tcaaattgat tatataaata gtattaaagt agttgataaa aaagattata aaaatggtat
    12301 agataatctt cctaaatctg atgttttaaa atactttttg gaagatgaat cttgggtagc
    12361 tataagacca tctggaacag aaccaaaact taaattttac atagctgtaa aaggagctag
    12421 tgatattgaa gcagataaaa agattcaagg tttgaaaaag tatatagatg acatggtcga
    12481 aaaattaaaa taaaatatta ttattttata agataatttt gtaataatat ttatttagct
    12541 gaaaattata atttactttc attgagtaga atatggaaat gttagtagaa ttgttatttt
    12601 cagcttttat ttgtgttatt tttacttaaa taattatatt atagaatgaa tctgagtata
    12661 attaatattg tataatgaaa ttaatttgat agaaaagggg atatttatgt ttggtataga
    12721 taagaaattg attgaatata taattgcaaa atccaattac agtgaattaa gttcaaagtc
    12781 agcagaagta tctttcttct taatgttgtc catatttcct tttttaatat ttacaataag
    12841 ttctatagct tatataccga ttcttcattt aaataagtat atagctttgt ttagaaatat
    12901 gatgccagaa ggtgcttttg ctgtgttatc ttcaatcata gtttctgcaa tagacaatag
    12961 aaacctgaaa tttttagcag tcagttttgt acttactatg tggacatttt caagagcagt
    13021 aaaagctctt attaaaggaa tgaatagagc ctataaagtt aaagaaacta ggtctttttt
    13081 taaaatttta tctatatcat ttttatttac aataatgctt ttggtcctta tttttttatc
    13141 tatgatattt ttagtttatg gggaaaaaat aggttatttt atttttaatc ttgtaggatt
    13201 ggatgagata tttataaaaa tatgggatat tctaagatac actgtaggta taataacaat
    13261 aatagtgata tttacacttt tatataaata cactccaaat aaaaagctaa ctattaaaga
    13321 atctgcgcca ggtgctattt ttgctacttt tgcttggttt ttagtatcat ttttatattc
    13381 ttattataca aattactatg ctaactatga agtaatctat ggaagtatag ctgaaataat
    13441 tgtacttatg acctggatgt attttagtag ttggtctatc gttattggct atgaggtaaa
    13501 ttcaagattg tattttagaa agataagaca tgaaatgtta aagtaaagta tttaaaataa
    13561 aagagagtat ttagtattat aaatgtaatt gaattaataa agaaatattg acatttaagt
    13621 taaaaagtct agaatttttc tgggcttttt ttatttttag tataacttga caaattttat
    13681 ttaatgggat agttttaatt tataatcatc tttaaaaaga tgactattga taagtaagag
    13741 ataaaattgg taagtatcaa aaaatatact aaataaaaat tatagtccta atgagttaat
    13801 ttctgtattt tcaaataatg cagtatttag tcatgtgttt ttatatgcgc ctgtatttct
    13861 aattaccaat atattagaat aagctactgt aattagattt agaaaaaagc gacataaata
    13921 attataaaca aataataaat tacataaaat taaatttatt ca
//
LOCUS       DAMJYC010000102        13730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJYC010000102 DAMJYC010000000
VERSION     DAMJYC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.106"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            114..254
                     /locus_tag="PC472_003198"
     CDS             114..254
                     /locus_tag="PC472_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728503.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(429..749)
                     /locus_tag="PC472_003199"
     CDS             complement(429..749)
                     /locus_tag="PC472_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2728504.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(869..1606)
                     /locus_tag="PC472_003200"
     CDS             complement(869..1606)
                     /locus_tag="PC472_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2728505.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1821..3644
                     /gene="acd"
                     /locus_tag="PC472_003201"
     CDS             1821..3644
                     /gene="acd"
                     /locus_tag="PC472_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2728506.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3859..8157
                     /locus_tag="PC472_003202"
     CDS             3859..8157
                     /locus_tag="PC472_003202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2728507.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIAQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8361..8822
                     /locus_tag="PC472_003203"
     CDS             8361..8822
                     /locus_tag="PC472_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2728508.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8852..10006
                     /gene="nusA"
                     /locus_tag="PC472_003204"
     CDS             8852..10006
                     /gene="nusA"
                     /locus_tag="PC472_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2728509.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10013..10294
                     /locus_tag="PC472_003205"
     CDS             10013..10294
                     /locus_tag="PC472_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2728510.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10284..10595
                     /locus_tag="PC472_003206"
     CDS             10284..10595
                     /locus_tag="PC472_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2728511.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10617..12557
                     /gene="infB"
                     /locus_tag="PC472_003207"
     CDS             10617..12557
                     /gene="infB"
                     /locus_tag="PC472_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2728512.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12600..12980
                     /gene="rbfA"
                     /locus_tag="PC472_003208"
     CDS             12600..12980
                     /gene="rbfA"
                     /locus_tag="PC472_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2728513.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12961..13638
                     /locus_tag="PC472_003209"
                     /pseudo
     CDS             12961..13638
                     /locus_tag="PC472_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5487 a         1559 c         2579 g         4105 t
ORIGIN      
        1 tgtcaaaaaa taaaaaaaat tttatttatt agagtttttg gaaataataa taacagatat
       61 tataattaat taacagtttt tgtaagtatt tgacaataga gagggtgtaa caaatgggaa
      121 taatagagtc agcatcaaaa ttggctgaga tggttcattt attggctgtt gaaaagggga
      181 taactgatat agaagcatgg gatgaagctg tgaaagaata cagcaaaatt tatgaagaaa
      241 gaagaaatga atagaatgat aaaaaatgaa aacttgatga taaaaaataa ctttatttaa
      301 agaaattcaa tttttataga agtattaaaa ataatataaa taaaaagatg gtatttcaaa
      361 tgtaagtaaa aaatcatttt gggataccat ccttattatt tattttaata atatataagt
      421 aataaatctt acttatccat attttttatg taacctcttt tttctagctt ttttatatgc
      481 tccttacgta tttcatcaag attaatacca tattgttctt ctagcttaaa catcatagta
      541 acacatgtct gcgctacatc cattagttcc ttagcagttt tctttataat atcaacttca
      601 tctaaatcat tattttctcc acttagattt ctgaatttac ctatacattc agctaattca
      661 ccttgttctt cacccatttt gataaatgta gattctagtg ttgggtctaa attattaagc
      721 tctggtaaag atatagtttt tagtttcata aaagctcctt tcaagttatt cgtatttata
      781 ctaaaacatc ataacaaaaa taatactaaa aaaaaacttt aaattcaaca taaaaactta
      841 catatataat aaaaatcgtg aaataaatct atatttgtct taatacagcc tcatataaaa
      901 cttctccaat aggtttattt attacaagag aagctgactt atcatagact gtatttgctt
      961 tattaataag agcaatataa tcacctttgt aataatctat aaaacttgct gctgggtaca
     1021 cagctaatga tgtacctcct ataatcaata aatcagcatt acttatagca gaaattgttt
     1081 tagtaatgac atcactgtca agagcttcct cataaagtac tacatctggt tttactatag
     1141 aaccacagtt gtcacagtaa ggaatattac ctcccagatt taacatagat tctaagtcaa
     1201 aaaatttacc acatttagta caataatttc tatgtacaga accatgaagt tctagcacat
     1261 ttttacttcc agccatttga tgaagtccat caatattttg agttatgaca gctttaagct
     1321 tacccatttc ttcgagtttt gctaaagcta tatgagcatt attgggtttt gcattaggat
     1381 atattaattt atccttataa aattcaaaaa attcttctgg atatctaaca aaaaaagtat
     1441 gtgaaactaa ttgttcagca gtaaattgtt tattgagttt ttggctaaat aatccagtag
     1501 aacttctaaa gtctggaata ttagattcag ttgaaacacc tgctccacca aaaaatacaa
     1561 tattattgtg attagctatt aagtctttta agctatttgc atccatagtt acacctccaa
     1621 attattatat ataaattgta ctacatatta ggtcgtttat gttttattaa tttaaataaa
     1681 tatatagttt aataagttaa ataaagggaa tataaataaa atgtatacag agtataaata
     1741 gacaatattt actaaaataa caagttgtcg tataattata ttaaaattgt tttagtgtat
     1801 aagaagggcg ggggaagtac gtgaagaaaa aagcagcttt agcaacattg gctatgttac
     1861 ccttaggtgt ggtaaatgca catgctgatg gagatatagg tatagtgact ataaattatt
     1921 taaatgtaag aaatgaacca actgccgaaa gtagcatagc ctttgttgct aaaaaagatg
     1981 ataaagtttt gattaaagac tcttctaatg gatggtataa aataaaagct gaatctggac
     2041 aagaaggttg ggcttcatca aaatatatag caaaatcaaa tagtgactct ttaagaacgt
     2101 ctacaaataa agaaaaacaa gttatttcaa atagtttaaa tatgagaaat ggagcaggta
     2161 caagttacag agtaataact gtactaaaaa aaggtcaaaa agtagaagta atatcagaga
     2221 gtaatggatg gtctaaaatc aaatatgatg gaagactagg atatgtatct agctcttatt
     2281 taggagacgt ttcaaactca actaataaat caaaaactaa acaagtgaat acaacttcac
     2341 taaatgtaag gagtggacca aatacaagtt atggtttgtt aggcaagttg ccaaaaggaa
     2401 gtaaagtaga agtaatatca gaaagtaatg gatggtcaaa aataaagtat aatggaaaag
     2461 atgcctatgt atctagtatg tacttatcgg atgtaagtca aagtaattca gacaattcta
     2521 gtcaaagcaa tgataaaaag aatactgata aggttgtaaa tacagcttct ttaaatgtaa
     2581 gaagtggacc aggttctaca tatagtaagt tgggaaaagt ttataaagga agtaaagtaa
     2641 ctgtactatc agaaagtagt ggatgggcta agattaattt taacaataaa gaagcatttg
     2701 tagtaggcaa ttatttatct acttcagcag atacttcaaa taataactca aatagtaact
     2761 ctgataacag ttctaatagt aatggcaata attcttcatc atcaggtcaa gtaaatggta
     2821 tgtcaggtat aagtggggct aaaattgatt ataaatcttt gagttatact ttagaatctc
     2881 atataagtaa acaagttgag aaagcagcat caggaggaaa tgtgatagct ccaagtaata
     2941 ggaaaagcac tccaagtcct gaatttagta ctttttcagc tcaaagaaca agctcatttg
     3001 taaatgcaag ttcaagtgat atagaatatt atttaaatcc taaaaatttt acaaatacta
     3061 ctaaaggtat gatgcagttt ttaaagatta acagttatag agatggtatt tcagaatcaa
     3121 gtcttaactc gtatcttaat ggtttatctt ctagtgtatt taaaaaccag ggagcagcct
     3181 ttataaatgc tgccaagaag tataatatag atgttgtata tctagtatct catgctatgt
     3241 gggaaactgc ttatggtaag tctacacttg cccaaggtca aacattaaca tcttataagg
     3301 gacaacctct tagtaagcca gttaaagtat acaatttttt tggtatagga gcaatagata
     3361 aaagcgctaa tgtttcaggt gcagaagcag cgtattccaa tggctggact agtgtagaag
     3421 ctacaataga tggctctgca aagtggatat cacaaaatta cgtaaatagt tctaagtaca
     3481 atcagaatac tatctacaag atgaagtgga attatgatta tacatggcat cagtatgcaa
     3541 ctgatgtaaa ctgggctaat ggaatttctg gaattatgga aaatttaatt ggtctttatg
     3601 gaggaggaag tagtttggtt tttgaagttc ctcaatataa gtaatacata aataaaaaat
     3661 atagactatg ggtagttttc attttattta aattatctat agtctttttg tatataattt
     3721 ataatctgta aatttaatgt taacaaaact tttatgttat aatattaatt ctaagtaaat
     3781 ataagtgagt aataggtaga aatagataaa aagtggtaat aatatatata aagataattt
     3841 tgagaaagga ggatatatat ggaaagtatt aaggaatatt tggataagtt agaaattaat
     3901 aatagtggtc ttggaaagca acttaaagaa gtttatataa atagagtagt ttattttaag
     3961 gaagataaga tagtttattt ttatctaact tctaaagata ttgtttctca tgaactttta
     4021 gataaattta aagaagaatt gatgtataaa ctcgattatt ttaaagatat gagaatgaag
     4081 ataagattta ctggtcttga aagaaaatcc aataaagatg ttataaaaaa atattggaat
     4141 aatatattgt atatattaaa gtatctttgt ccatctattg cagggtggta taaacaagta
     4201 gaatttcttt gtttagaaga agaattaaaa ataaaacttc caaaaggtat attttatgag
     4261 cgattaatga aaaaaaatgt aacttatgta cttaaaaccg tgcttagcga agagttggga
     4321 ttagatttaa atataacaat agaaaaagct gttgatgaaa aggtaaataa agaaagactc
     4381 ataagaatca atgatagaga aatggaagaa aaaattaggg ctttagaaat tggaaaagtt
     4441 aataactgtg aagaaaatga agaggaaagc tatgtcatta agtctgatgt agatgaaaat
     4501 ttaatctatg gagataatgc aaatgctatg attgaaaata tagttgaatt aaatgcatct
     4561 tctggaacag ttgcagttgt tggagatata tttgatgttg acactaaaga acttaaaaac
     4621 ggaaaaatac ttatgatagc ttcaattaca gattatacta gctcaataag ctgtaaactt
     4681 tttttaacag atacaaataa ggatggagta cttgaaaatg ttaaaaaagg agcatactta
     4741 aaaatcaagg gtgacatagt ttatgataca tatcaaagag aaattagtat gatgattagt
     4801 gggataagaa aagaaactag aatagagaga gaagataaat cagaggaaaa aagagtagaa
     4861 ctccatgcac atacacaaat gtcttctatg gatgctattt gttctgttaa aaagcttgta
     4921 gagcgtgcag ccaaatgggg tcattctgct attgctataa cagaccatgg tgttgtacaa
     4981 ggatttccag atgctatgaa tgcaggtaag tcaaataata taaaaatact ttatggagta
     5041 gaagggtact tggttgaaga tgattctctc attatagagg atgcaaatga taaagaactt
     5101 tcgcagacat ttgttgtatt tgacatagag acaactggtt tttctaatac aaatgataaa
     5161 ataactgaga ttggtgctgt aaaaatagaa aactttaaga ttgtagatag atttagtgag
     5221 ttaataaatc ctgaaaaaga tatttcttat aagatacagg aattgacagg aataactaat
     5281 gaattaatta aagacaagcc aaccattgaa gaagttcttc ctaagtttat ggaatttgtt
     5341 ggagatagtg tattagtagc acataatgca gagtttgata ccggatttat atcgcaaaaa
     5401 tgtagggaac aaggcttgtt atataataac aaaaaagtag atacacttat gttagcaaga
     5461 gtaatgttgc caaacttaaa aagacataga ttaaatgtcg ttgcaaaaga acttggtatt
     5521 cctcttttaa atcaccatag agcagtggat gatgctgagg ctacagcact tatatttaat
     5581 aagtttttgc agatgcttac agaaaaaggt gcaaaaactc taagtgatgt aaacaatata
     5641 cttggaaaga ttgattacac aaagcttagc acaaatcata taaccttgat agcaaaaaat
     5701 tctataggta taaaaaattt atataaaata atttcagatg cacatgtaaa tcattttttt
     5761 agagcaccaa gaatccttag aagtgtatta aatgaatata aagaaggatt aataattggt
     5821 tcggcttgtg aagctggggt ggtttttcaa gctgtaaaga aaaacgtgtc tgatgaggaa
     5881 atgaaaaaaa taatagattt atacgattat attgaagtaa tgcctataga taataataga
     5941 tttatgattg ataaaggaga agtaaaggac gaagaagagt taagggagtt gaataggaaa
     6001 ctaattgata cagccaaaaa atttgacaaa attccagtag ctactgggga tgttcatttt
     6061 ttagacaaac atgaagctgt cttaagaaaa gttcttaaat attctcaagg ttttaaagta
     6121 gatgaagaag aaacttattt acattttaga acaacagatg aaatgttgga agaatttagt
     6181 tatcttggag aagaattggc atatgaagtt gtagttgaaa atagtaatct gattgccgat
     6241 atggttgaag atataaaacc tataccagat gatacttatc ctccaattat agagggctca
     6301 gatgtggagt taagagaaat gtgttacaaa aaagccaaac gaatctatgg agacccaata
     6361 ccagaaatag ctcaaaaaag actggataga gaattaaatt caataataag caatgggtat
     6421 gcagttatgt atattatagc tcaaaaactg gttgcaaaat ctctttcaga tggatattta
     6481 gttggttcaa gaggctcagt tggctcatct tttgcagcta ctatgagtga tataacagaa
     6541 gttaatcctc ttccagcaca ttatatttgt gaaaacgaag actgtaaata ttcattcttt
     6601 tatgaaatag gtgaatgggg ctcaggtgtt gatttaccag acaaggattg tccaaagtgt
     6661 ggaagaaaac ttgctaaaaa cggtcatgat attccatttg aggttttctt aggttttgaa
     6721 ggtgataaag aaccagatat agacttaaat ttctcaggaa attatcagcc aacaattcat
     6781 aaatacacag aagaactatt tggagagggg tatgtatata gggctgggac tataggtact
     6841 gtagcagaaa aaacagcttt tggatacgca agaaaatatg tggaagaaaa taatataagt
     6901 gtaccaaatg cagaagtact taggctttca aatggatgta ctggagtaaa aagaacttca
     6961 ggacaacatc ctggtggagt tatggttata ccagactata aagatgtata cgattttaca
     7021 cctatacagt atccagcaaa tgatacttct tgtggtgtta taactactca ctttgattat
     7081 cattcaataa gtggtagaat acttaaactt gatatacttg gacacgacgg tcctaccatt
     7141 ataaggatgc ttgaagatat gactggaata aatataacag aaattccttt agatgataaa
     7201 gagactatgt ctctatttac atctacagaa gcattaggtg ttacaccaga agaaataaac
     7261 tgtcctatag gttgccttgc tgtaccagag tttggaacta agtttgtacg acaaatgtta
     7321 cttgacacaa aacctactac atttgcagaa cttgtacgta tatctggtct ctctcatggg
     7381 acagatgttt gggtaaataa tgcacaagac ttagttagag aagatatagt tggattaaaa
     7441 gatgttatat ctacacgaga tgacataatg aactatttaa tatttaaagg attacctccc
     7501 aaaatgagct ttacaataat ggagagtgta agaaaaggta aaggtttaaa accagaacac
     7561 attgaagaaa tgaaaaaaaa taatgttcca gaatggtata taggctcatg taaaaagatt
     7621 aagtacatgt tcccaaaagc acatgctgtt gcttatgtaa tgacatcatt taggattgca
     7681 tattgtaaag ttcactatcc agaagcattt tatgcgactt attttacgac aaaagttgag
     7741 gattttgatg cagatttaat tgttaaagga ttagatgcta taaaatcaaa aataaatgaa
     7801 attgagtcac tgggaaatga tgcaaccaca aaagaaaaag gtatgttgac cgttttagaa
     7861 gttgctcttg aaatgtatgc tagaggaata aaattattac cagttgatat atataaatca
     7921 gatgcaactg agtttatagt tgtaggagaa aaaacattac ttccaccaat ggcagcaata
     7981 caaggtcttg gaggaaatgc ggctataaac atccaaaatg aaagaaaaaa tggtgaattt
     8041 atatcaaaag aagatttgag aaagagaaca aaaatatcaa aaacggtaat agaaactctt
     8101 actaatcatg gttcacttga gaatatgagt gagagaaatc aattgtcatt gttttaagtt
     8161 agcccaaaaa gttgcaaata aaagaatttt atgatatcat agtatagaaa atagtatact
     8221 ttgcagaaac taagagtgag acagctgttc tcactctttt attttaaata tattattaaa
     8281 aagctggttt atttttatta aatatatttt atttaagatt atataaactg gtcatttaca
     8341 attgaatagg aggaacttta atgaaaaaaa atttagaagc tacaattgag gagattgtaa
     8401 ctaaaataac ggatgaacat ggatttgaaa tggttgatgt tgaatatgta aaagaagcag
     8461 gagaatacta cttaagagta tacatagata aagaagaagg aatatcttta aatgaatgtg
     8521 aattagttag tagagaatta agtccaatac tagatgaaaa agaccctata aaagaaaatt
     8581 actttttaga ggtatcttct ccaggacttg atagagcatt aaaaaaagac agagattttg
     8641 ttagatatca aggtagagat gtagatttaa aattatataa accattaaat ggatgtaagc
     8701 aatttgaagg tgaacttgta ggattaacag aagataataa tataaaaatc attgcaaatg
     8761 gtaaggaaat ggaatttaat agaaaagatg ttgctattgt cagattagca ataaagtttt
     8821 agtggtatat taaataggga ggatataaaa aatgaatcat gaatttatgg aagcattaga
     8881 tgaattagta gttgacagag gtatagataa agaaatactt atagatacta tagaacaagc
     8941 tcttttaact gcatacaaga aaaactttgg ttcagcacaa aatgttagag ttgaatttga
     9001 tagagacaag ggagatataa aagtattttc tcaaagagtt gttgttgatg aatcagattt
     9061 atatgatact ttcttagaaa tagagttagc agatgctaga gagataaacc ctaattatga
     9121 attaggtgat attatagaac atgaagttac acctaaaaac tttggaagaa ttgcagctca
     9181 aacagcaaag caagtagttg ttcaaagaat aagggaagct gagagagaaa tagtgtataa
     9241 tgaatttata gaaaaagaaa atgaaatagt tacaggagag gttgctagag caaataaaaa
     9301 tgttgtttat gtaaatcttg gaagaataga aggtataatg actcaaagtg aacaaatacc
     9361 atttgagaat tataaagcag gtcaaaaaat aaaattctat atacttgaag ttaaaaagac
     9421 taacaaagga cctcaaatag ttgtatcaag aagtcatccg ggtctagtaa agagattgtt
     9481 tgaagaagaa gttcctgaaa tatatgaagg aatagttcaa ataaaatctg tagcaagaga
     9541 agcagggtca agaactaaaa tggcagtaag atctatagat gaaaaaatag acccaattgg
     9601 agcttgtgtt ggaccaaaag gacttagagt aaaaaatata gtggatgaac ttggcgatga
     9661 gaaaatagat ataataaaat atagtgaaga cccagcagag tttatatcag cagcacttag
     9721 cccatcaaag gttgttagag tagatataga tgaagatgaa aagtctgcct tagtaatcgt
     9781 tccagattat cagttatcgc ttgctatagg taaagaaggt caaaatgcaa gactagcagc
     9841 aaaattgact aactggaaaa tagatattaa gagcgaatct caagcagaac aagaaatgtt
     9901 aaatcttgaa aattccaaag aaatagaagc tgaggatgtt gaagctgaat ttatcgacca
     9961 agaagagtta gatacttcag aggataattt tagtgaagaa gagtaaggag ggatgatttt
    10021 gaaaaaagta aaaaaagtgc ctcaaagaaa atgcatagca tgtcaagata gagactctaa
    10081 aaaagagctt ataagaatag ttaaaaataa agaaggaaaa atatttttag acccaacagg
    10141 aagagccaat ggaagaggag catatgtatg taaaagctct gaatgtctaa aaaaagctat
    10201 aaagagtaaa gcattaaata aagcttttaa aatagatgtt cctgatgaag tctatgataa
    10261 tctacttatg gagttagaag aatatgaaaa ataataaaga aaaagtactt tcgttcttag
    10321 ggctcgcaac tcgtgcaggg aaaatagtgt ctggtgatga ttcaactttg cttgacctta
    10381 aaaaaggaaa agtaaatttg attttaatcg cagaagatgc atcaaataat actaaaaaac
    10441 tttttaaaga taaatcaact tttagaaata ttccatatct atttttttca acaaaagagg
    10501 aaataggatt tgcaatagga aaatctccaa gagcagttgt aggaataaaa gatgaaaatt
    10561 tttctaaaaa gataattgaa ctaatagaaa tttaaaataa aatagggggt gatcttgtgt
    10621 caaaaacaag agtataccaa atagcagaag aattaaatat atcaaatgaa gagcttataa
    10681 ataagttagc tgaattagat ataaatgtaa ctgacaaaga ttcagtatta gaaggtgaag
    10741 agttagaatt agcacttgaa atgttaggtg aagatttatc tcaagaaaat ggaaatgtta
    10801 tagaaataga tggaaagttg acagtacaag tattagcaac gaaattagat aaatctccat
    10861 cagaaataat tatgaagctt atgaaaatgg gaactatggc aacaataaat caagagataa
    10921 gttttgagat tgctgctttg gctgccaaag actatggatt tgaattaaca gtagctgaaa
    10981 gtgatgatac agaagcttta gaaatagaag ctttaatgga aattgaagaa gataaagaag
    11041 aggatttaaa acctagacca ccagttgtta cagttatggg acatgttgac catggtaaga
    11101 catctttact agatgcaata agaaaaactg atgtgatttc aggtgaagct ggaggaataa
    11161 ctcagcatat aggtgcttca gaagttaaaa tcaatggaca caaaatagta tttttagata
    11221 ctccaggaca tgaggctttc acatcaatga gagcaagagg agcacaagtt acagatatag
    11281 caatattagt agttgcagca gatgatggta taatgcctca aacagttgaa gctataaacc
    11341 atgctaaagc agcaggtgta cctttaatag ttgctattaa taaaatagat aaaccaggtg
    11401 caaacccaga taaggttaaa caggaattag cagaccaagg attattagtt gaagactggg
    11461 gtggagaagt aatagcagtt ccagtatcag ctaagaaaaa agaaggaata gatacacttt
    11521 tagaaatggt attattggtt gctgaaatgg aagagttaag agctaatcca aataaaagag
    11581 cagtaggaac tgttatagaa gctgagcttg ataaaggaag agggccagtt gcaacagtac
    11641 ttgttcaagg tggtacttta acagtaggtg acccaatcgt tgcaggtgtt gcatgtggta
    11701 aagttagagc aatgataaat gcaaaaggta agagagtaaa aacagcagga ccatctacag
    11761 cagtcgagat attaggtcta tcagaagttc cacaaggagg agaccaattt gtcgaagttc
    11821 caactgataa gattgctaga agtgtagcag caagaagaca acaaatagtt agagatgaaa
    11881 tgttaaaatc aactcaaaga ttatcactag atgctctatt tagtcaaatg agtgaaggtt
    11941 ctataaaaga ccttaatata gttataaaag cagatgtaca aggttcagta caggcagtta
    12001 aacaatcttt agaaaaatta tcaaatgaag aagttcaagt taaagtaata catggtggtg
    12061 ttggagctgt aacagaatca gacatattac ttgcagcagc ttctaacgca ataataatag
    12121 gattcaatgt aagaccagtt ccaggtgcag aatctctagg agaaaaagaa aatgttgata
    12181 taagaacata tactataata tacaaggcaa tagaagatat acaagcggct atgactggta
    12241 tgttagaccc tgaatatgtt gatgaagaaa caggaaaagc tgaaataaga gaaatatata
    12301 aaatatctgg tgttggaaca gtagctggat gttatgtgac taatggtaag atatttagaa
    12361 attgtaaagt aagattagtt agagatagca taataataca tgaaggtgag cttgcagcac
    12421 ttaagagatt taaagatgat gtaaaagaag ttaattctgg atacgaatgt ggtatgagtt
    12481 ttgtaaatta taacgacata aaagaaggcg atatagtaga agcttatata actaaagaag
    12541 tagaaagaaa attataaatt cgtatcaaag aaaatcagtt agtaaagaag gtcgatggta
    12601 tggcatcata taatagaaca agaaggatag cagaagaaat aagaaaagtc gtaagtacta
    12661 tgcttataaa tggagtaaaa gaccctagaa taacatctat ggtgagtgta actgatgtag
    12721 aagttacaaa tgacctaaga tatgcttatg tatatgtaag tattctaggc ggagatgaag
    12781 aatccacact aacaggtctt aaaagtgctg gaggatatat aagaagagaa gtaggaaaaa
    12841 atataaaact tagatatatt cctgagatag tatttaaact tgatgattca atagaaaaag
    12901 gaatgtatat ggatagcctt ataaaaagag ttaatgaaaa aaatgctcaa caaaatgagg
    12961 atgaaaatta tgatgaataa tatggatagt attataaact atatttatga gggtgactat
    13021 tttatagtta cctctcatat cagtccagat ggagacaata ttggttctac actttcaatg
    13081 tattatacat tgaagaattt aggaaaaaat gtatattatg tacttgatga tgaagctcca
    13141 ttaaatttaa ggtttttggt tgaaggtgta aaaatctata agtctaatga atttaatatg
    13201 aagaactatt ctctaattgc acttgattgt ggagataaga tgagaatatg tgtgagtgat
    13261 gaaatcaaaa ataatgcatc aaaattaatc tgtattgacc atcatgctag taatgattca
    13321 tatggagatt taaattatgt agatattgat gcatcttcta catgtgaatt agtttataat
    13381 ttattagtta gatttcagca aataaaagat attaatatta taaatgaaga tatagcaaca
    13441 tgtttatata caggtcttgt aactgatact ggaaactttt cgtattcaaa tgtacatgca
    13501 agtagctttg aaatggcaaa aaatttactt gttttaggtg cacaaaaaaa tactataata
    13561 caaaatatat atcaaagcaa ttcttctggt tattacaaat tattaggaga agctctaaag
    13621 ggtttggaga tatttgatta aaaaatagtt acgcacactc gtgactttag tcatgagtta
    13681 gtagctaaaa tagtcagcat atagggaaac ttatatgtag agataggata
//
LOCUS       DAMJYC010000103        13685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJYC010000103 DAMJYC010000000
VERSION     DAMJYC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.107"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            285..2384
                     /gene="nrdE"
                     /locus_tag="PC472_003210"
     CDS             285..2384
                     /gene="nrdE"
                     /locus_tag="PC472_003210"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2728514.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2397..3368
                     /gene="nrdF"
                     /locus_tag="PC472_003211"
     CDS             2397..3368
                     /gene="nrdF"
                     /locus_tag="PC472_003211"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2728515.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3444..4070
                     /locus_tag="PC472_003212"
     CDS             3444..4070
                     /locus_tag="PC472_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2728516.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4465..5094
                     /locus_tag="PC472_003213"
     CDS             4465..5094
                     /locus_tag="PC472_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728517.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5095..5877
                     /locus_tag="PC472_003214"
     CDS             5095..5877
                     /locus_tag="PC472_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728518.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5870..6748
                     /locus_tag="PC472_003215"
     CDS             5870..6748
                     /locus_tag="PC472_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728519.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6781..7740
                     /locus_tag="PC472_003216"
     CDS             6781..7740
                     /locus_tag="PC472_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2728520.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKGLIYNAK"
     gene            8054..8746
                     /locus_tag="PC472_003217"
     CDS             8054..8746
                     /locus_tag="PC472_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728521.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8756..9760
                     /locus_tag="PC472_003218"
     CDS             8756..9760
                     /locus_tag="PC472_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728522.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSKHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            10008..10859
                     /locus_tag="PC472_003219"
     CDS             10008..10859
                     /locus_tag="PC472_003219"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2728523.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            10963..11709
                     /locus_tag="PC472_003220"
     CDS             10963..11709
                     /locus_tag="PC472_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728524.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11709..13625
                     /locus_tag="PC472_003221"
     CDS             11709..13625
                     /locus_tag="PC472_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728525.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDTKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     IKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         5376 a         1330 c         2450 g         4529 t
ORIGIN      
        1 ttgtattgtt ttatatttga gttgataaaa atttctttaa ataaaaataa tgtcaattat
       61 aaagtaataa aagtttagat ataaaaaaat ttgtgtatat actatatgta gtatataata
      121 gataaagcac tgactatatg tagtgctttt gtttttgaaa aatacttatt gcatttctat
      181 aattatagat taattttggg atttcaactg ataagtacca aaagaaataa taagcaaatt
      241 aagtgaaatt atggcacaag attatgttaa aaggacggta aataatgaat tggatagagc
      301 ttaacaatca agtgataatt aaaaatgaac aaggcaagta ccaattggaa aaggataagg
      361 aagccttaag tagttacata gaagaattta tacagccaaa gttgagaaaa tttaataacc
      421 tagaagaaag attgaagtat ttaatagatg aaggttatta ttcaaaagaa gtaataaatc
      481 agtatagcat agatgttatt aaaaatatat atgatatgat tgataaacat aagtttgaat
      541 ttcaatcata tatgagtgca aataagtttt atcaaaatta tgcattaaag agcaatgatg
      601 gaaaggaaat attagaaaca tataatgata aagtgttaat tgtagctttg acactaggaa
      661 atggcgataa agatttagct ttaaacttgg cagataaaat agtaaaacaa gaatttcaac
      721 cagctacacc tactttttta aatgctggaa gaaaaagggc tggagaaatg gtatcatgtt
      781 ttttactatc tgtagaagat agtacagaag gtatatcgta tgctatatct tcatcaaatc
      841 acttatcaaa gattggtgga ggagttgcac taaatttatc taaacttaga gcatctggcg
      901 aatctatcaa agatatagaa ggtgcagctg gaggtgttgt tggagtagct aaaatgttag
      961 aacaatcatt tagttacttt aatcaaatgg gagctagaca aggttctggt gcggtgtacc
     1021 ttactgtatt ccatcctgat tttgaattgt tgatggatac taaaaagata aatgcagatg
     1081 aaaaaataag acttgctaca ttatcattag gagctataat accagataaa tttatggagt
     1141 tggcagaaaa aaatgaagta gcatatgcat tttatcctca tactgtgtat aagaaatatg
     1201 gagtttctct tgatgagata gaaatggatg agtggtatga taaattggtt aatgatagtg
     1261 atataagaaa aaaagaaata aatcctaggc agatgttgac aaaaatagca cagatgcaac
     1321 aagaaagtgg atatccatat gtagtgtaca tagatacagc taatagagaa catacattaa
     1381 aagatgttgg aattattaaa atgtctaact tatgttgtga gatatttcaa tatcaaacac
     1441 cttcagaaat agaggggtat ggaggaaaaa atgaatgggg acaagatata agttgtaacc
     1501 taggttcttt aaacatagct aatgtaatgg ataataaaac tatagaaagt acagttgaaa
     1561 cagcaataag agcactgtca tttgtagcag atagcacaga tataaaacca gtgccaacgg
     1621 taagcaatag taacagcaag tctcattcta taggattagg agctatgaat ttacatggat
     1681 acttagtaag agaaaatatt ttatatactt cagatgatgc tatagatttt tctaatgtat
     1741 tttttgcaat ggttagatac tattcaatta aagcttctat gaaaatagct atagaaagaa
     1801 atcaaacttt tgaaggattt gataaatcag aatatgttaa gggaagaaat agcaaggttt
     1861 tatcaaaata ctatgaacaa tcttatctac ctaagtcaga aaaggtaaga gcattatttg
     1921 aagggattta tataccaaca aaagaagact ggacaaaatt attggatgag gtaaaagaaa
     1981 aagggatata taatgcatat ttaatggcag tagcaccaac tcaaagcatt agttatgttc
     2041 aaaatgctac atcaagtata atgccaatta ctgagccagt agaagtaaga acatatggag
     2101 actctactac aatatatcca atgccatttt taacaaatga taatatgttg tattatcaat
     2161 cagcatacag gatggatatg agaaaggtca tagatttagt ggcaacagtt caaaaccatg
     2221 ttgaccaagg tatatcaaca acactgtttg ttacagatga aaagacaact agagatatag
     2281 ctagacatta catatatgcc tataaaaaag gtttgaaaag tttatattac acaagaacaa
     2341 agatgacaag agatactcat gagtgtctag tgtgttcagt atagggagag aaatatatga
     2401 catttaattt acataaaata cataatgcag ttaactggaa tagagaagaa gatggattta
     2461 cacaagcttt ttgggaacaa aatgtaaaac aattttggct tcctgaagaa atatctgttt
     2521 ctaaagacat aaaggtatgg aatgaactaa gtaataaaga aaaggaatta tataaaaaag
     2581 tattgggtgg tttaacatta ctggatacaa aacaggggaa taatggaata ccatctatga
     2641 tgagtttaac agaaaaccta caaagaaaag ctgtcttgtc ttttatgggg actatggaag
     2701 aaatacatgc aaaaagttat tcttctatat ttatgactct actttctaat ttagaaatag
     2761 atgaattatt tgaatggata gaaacagaac caacattaca aagaaaggct gatttagttt
     2821 tagctcaata cgaaaatact acaaatcaag agggattata cctatcaatg gttacaagtg
     2881 tatttttaga gagtttcttg ttttattcag gattctttta tccattgtat ctatctggtc
     2941 aaggaaagat ggtagctagt ggagaaataa tatcgcttat tcttagagat gagtctttac
     3001 atggtaagta tataggattg ctagcacaag aaatatacga ttcatttgat aaaatggata
     3061 aggagatgtt agaagagaag atgtattcta tactttatag ccttatggag aatgagatag
     3121 agtatacaaa tgttatatac agagaaagtg ggcttgaaaa agaggttgtg aactttttaa
     3181 aatacaatgc aaatagagct ttagaaaatc tagggtttga gaagctatat actgtagatg
     3241 ctataaatcc aattgtatta aatggtctta gcactgaaac caaaacacat gatttttttt
     3301 caactaaggg taatggatac caaaaaggag tgtatgagga attagaggat gaagatttta
     3361 taatataatt tataattatg acacactgtt gtcactttta gcgtgataat atcaatgtat
     3421 aaaatatata tggaggtatt tttatgaaat atgaatggag aaaaagagat aaagagttat
     3481 atttacctaa aaagaatcct gtaatagttg aaataccaga acagaaattt atcatgttaa
     3541 aagggagtgg agacccaaat tctaaagaat ttactgaagc aataggagta ctttattctt
     3601 tggcatatgc aataaaaacc atgcctaaaa aaggaattgt accagaaggg tattttgact
     3661 atacggtttt tcctttagaa ggtatatggg ataagggaga aaaaagtaaa ggcttagatg
     3721 tacttattaa agaagattta atttacacta taatgatacg tcagccagat tttgttacac
     3781 aagacctgtt ggataaagct actgatatag taaagaagaa aaaacctcat aatcttcttg
     3841 aaaaagttag atttgggtct attaatgaag ggctatgtgt tcaaatgctt catataggtt
     3901 catatgataa tgaaccagaa agctttgaca ttatgaacga attttgcaaa aaaaataaac
     3961 ttataagaaa atcttacaca catcgtgaaa tttatataag tgatgctaga aaagtatcgc
     4021 ctgaatcatt gaagacggtt ttacgttttg aagtagaaag ggaagtataa atattttgaa
     4081 taatttataa gagttataaa aagtgaagtc tgttgagact tcattttttg atgcaatttt
     4141 aaaattaaat ttgatgtttc ataatatttt aaaaactttc tacttttatt agtgtgaata
     4201 actgttgcta ttaaataata atactaatta tataagtatt attatataat atttatagat
     4261 tattatataa atagattatt agtaagatgt atgcattatt aattaaaata aattacatat
     4321 actatttaat atttcattta aaatattaaa aaatgtagat aaaaattgtt ttattaagaa
     4381 aaaattaata tattgacaaa atttaagtgc tcatttaaac tattaattga taataatatt
     4441 caatattaaa taataggagg cgttatgaat aaatatcttc taataaatcc agtggcagaa
     4501 agaatgtatg gagaaaagtt tcaccttatt aaagcaaatt tgataaaaaa ggggtacata
     4561 ataacagaat gtgaaccaca gttagaatat gtaaaaaagc agtacaaaga gtatacaaaa
     4621 aagactgaaa atacaatgtt ggattgtaga tgtccagaaa gtataagtct tttaaaaaga
     4681 aataatttga taaataattt tgaagtacct atgatagaac caatattagt aaggacttgt
     4741 agggttttat acgataaata tataaaaagt aaagatgata tgttggtagt aacctgtcca
     4801 tgtacacaat taagagactt tgcaagtgaa aagtttaagg ataagggtaa tgctatgttt
     4861 tatacatgga aagagtttgc tgaacaagaa ggtgtaaaat cacttggaaa aatagatgaa
     4921 tcaccaattc cattaggata tttcaaagat acttttgaaa aagttcttga agtatcaagt
     4981 gaagacgaaa tattaagtga aatacaaaaa ataagaaatg gttttgaaga taagtatgac
     5041 atagtagaaa tgttttattg taaagatggt tgtaataatg gagatggttt gtaaatggtc
     5101 aataaattta ataataagtt tttaaggcta gtaaagagtg ttctgatatt ttttataatt
     5161 cttttattat ggaaaattac aaattatctt ggtatttgga gtgattatat attaccatca
     5221 cctgaaaagg tatatagtac atttttaaat atgataagtg atggttcaat atttattaat
     5281 gtatatgcaa gtatgaaaag agttttgata ggttttgcaa taagtactgc tataggtgtg
     5341 cctttaggga tattctttgg aatttatagt ggagtatacg aatactttaa atctctaata
     5401 aattttttaa ggaatacacc tccccttgct ttaataccaa tgcttatatt atggtttggt
     5461 ataggagagg aatcaaaaat tataattata gttttagcat cttttttccc aatctttaca
     5521 agcacattga aagggattaa aaattgtgac tcaaaactga ttgaagtggg tagagtattt
     5581 gagttttcaa agcttcagat aatatttaaa ataataatac caaatgccat tcttgatatt
     5641 gctgttgggt taaagttagc tcttggatat agctttagag caattattgg ggcagaactt
     5701 gtagcagctt catctggtct tggatacttg atatctgatg gaaaagaaat gtctagaaca
     5761 gatgttgtta ttgtagggat aatcgtaatt ggacttttag gaataattac agattacata
     5821 ttttcgatta ttgtgaagaa ggtaagtaag ggaaagatgg tggaagcata tgaataattt
     5881 agtatgtgac tctggatata taattagaaa tttatataaa agttataaag ttgacaataa
     5941 agaacatgta gttcttagtg atatctcttt aaacatagat agaaaccata taacagtaat
     6001 tttaggtgaa agtggttgtg gaaagactac cttgcttaga gcaattgcat cacttgaagg
     6061 tataaatagt ggtgaaatta ggtttattaa tgataataaa gagtatagac ctaatgttgg
     6121 ttttgttttt caggagagta gattaatgcc ttggttaaac gtaagtgaaa atatagcttt
     6181 tcataatcaa agaaaaaata ctattttaaa caaggtgctt agaaaattta aacacaataa
     6241 aagaattagg agtaaaatta taaatgataa ttttagaagc ttagaaacaa atgataattt
     6301 gaacaaatca aataaatata ataggaaaag taatattgat gtagaaaaat atcttaagat
     6361 gatgaacttg cagaaattta aaaactctta tccaaatgaa ttgtcaggag gtatggcgca
     6421 gagagtaagc atagctagag ctctttcttt caatccagat atgttgctta tggacgagcc
     6481 tttttcggca ttagattatt ttacaaggat tgatatgcaa aatgaagtta taagaataca
     6541 taagtcaact aataaaggcg tgatatttgt aactcatgat attgatgaag cgttaaagat
     6601 aggcaagaaa atagttgttt tcacagacga aagaaaagta aaagaattta atatagaaga
     6661 tggttataat agagatttaa cttcagatta ctatattaaa ttaaaaaaag atattttaat
     6721 gacaatgaag tcaaataaag gcatttaaag aaattaataa tattttaagg agtaaagtaa
     6781 atgaaattaa aaaagctatt gataggtata acgatattaa cacttgcaac agcatcttta
     6841 gctggatgtg ctaaaaaaaa taatggagaa aaactttcag aaataaattt aacttatgta
     6901 aaatcaccat taaatgttcc ttctataatt cagaaacagg atgatttatt tggaaaggaa
     6961 tttaaaaagg ataatataaa agttaacttc cacgaaatta ctacaggtcc agagcaaaca
     7021 caagctttag ctgctggaga aatagacttc ttacatgcac taggtggtac atcagcttta
     7081 atagctgcat ctaatggagt agagttaaag atattaaaca cttatagcag gtcaccaaaa
     7141 ggatttatga ttttgactaa taataattca ataaaatcag cagcagattt agtaggtaag
     7201 aaggtggcag gcccaaaagg gactatatta catcaggtat tgatttcagc acttgataaa
     7261 gaaggattaa gtatggatga tgtggaattt gttaatatgg gtattccaga ggcatcagca
     7321 gctctttctg atggcagcgt agatgctgca ttgattgctg gtccagcagc tcttaaagct
     7381 atgaagtcag gtagtaaact tgtagcaaat ggtgaaggtt tagtagatgg tataatagta
     7441 actgctgtta gcacagactt tgcagagaaa catccagaaa tagttgaaag atttatgaag
     7501 gttgaaaagg aaactttaga atatgtaaat aataattttg atgaagctat ggaaaaagta
     7561 gctaaagaag ttgatttaag tcttgaagaa actaaagaat tatatgcttg gtatgatttt
     7621 agtttagata taacagataa agacatatct tcgcttgaag atacacaaga cttcttaata
     7681 aaaaataagt tacaagagaa aaaagtgaac ataaaaggat taatctataa tgctaagtag
     7741 aaactatgat aaaataatac taagtaagaa atataataaa agtttaagtt accttattaa
     7801 ggtaactttt tctattttga attagtaatt tcaatttatt ttagaatgat tagttgataa
     7861 taatgattaa ttaatcataa aaagatactt ttttatagag ttttattaag atgtataata
     7921 atatggttat acttatgaag tcagtaattg tgaagttagt aatatgatta aatttatata
     7981 ttgtaaaaag attataatat attaaaattg gactgttata tgatgagtaa ataggtatat
     8041 taggaggaat tatatgagtt ataaagtata tattgtggaa gatgatatat caataagtac
     8101 attacttaaa gattatattg agaaatatgg atttgaagtg attgttgaag agaactttga
     8161 tgatataatg attacatttg aaagttttaa gccacactta gttctattag atgtaaattt
     8221 acctaaattt gatggtttct actggtgtag atgtattagg caacagtcga atttacctat
     8281 aatttttata tcagctagag atggtaatgt agaccaaatt agagctttag aaagtggagc
     8341 agatgattat ataacaaaac ctttttacta tgatgtagta atggcaaaaa taaagagtca
     8401 tataagaagg gtatatggtg aatattcacc taaaatagac gaaagaatag tggagctaga
     8461 tggattaaag ttttatccag aaaggttaga actagaatta aatggtgaga atctaattat
     8521 aactaaaaaa gaaggtatac tgattgaatg tcttatcaag agatacccaa aagtagtaag
     8581 tagagattat cttttagaaa agatatggga tgatattgag tttgtagagg aaaatacttt
     8641 aaatgtaaat gttagcagga taaggaaaag atttgaggaa ttaggtatag ataatgctgt
     8701 tcagactgtc cgagggttag gatataaatt aaataagaat tggtaaggag aaagtatgaa
     8761 gttattttta aaagattata aaggttatat ttttatatat tatataggca tagttatgac
     8821 aattatttac tgtaatctta tgaagtttat tagttttgga gagagtttat atattctttt
     8881 atttagttct tttatattgt tatgtttttt aatatataag tattataaaa atagagaagt
     8941 atataatctg tttgaaaaag gagtacattc tttggaggag tcatttttgt atttaggaag
     9001 ctcattcttg gggagaaata tatcagttat tttaaaacaa caacataaat tgtatcaatc
     9061 aatgatacaa gagcataaaa agatacatag taaacattta actttcatca accaatggat
     9121 tcatcagatg aaaacaccaa tatcagttat aagccttcag cttcaagatt atgagggaga
     9181 agaagtaact gaaaatataa gaaaagaaat agatagatta aataaagggt tagatatggc
     9241 tatgcatttt gcaagggcag ataattttca gaaagacttt atagttgaaa aagccaaatt
     9301 aaatgaaatt gtatctagta ttgtaaatga agaaaaaagg ttatttataa aaagtggaat
     9361 gattcctaag attcaagtag acaaatttac ttatgtttat acagatgtaa aatggatgaa
     9421 atttataata ggtcaattaa ttacaaatgg agtgaaatat tctaaagatt atagtaacta
     9481 cctaactata aaatctgaag aaaataataa atatgtaatt ttaagtataa tagatgaagg
     9541 aattggaatt gtttctaaag atttaaaaag ggtatttgac ccatttttta caggagaaaa
     9601 tggtcgtaag tttggagaat ctacaggtat gggtctttat ctagtaaaaa aggtatgtga
     9661 tgatttaggt cataaagtaa gtataaaatc agaagtagga aaaggtacag aagtttcaat
     9721 ctactttaat aaagatgtaa atagtttaat aaaggtgtaa attatctgtt acaatagggt
     9781 ttttagttaa ttgtactttt ttattataca atttattaaa tgaaatattc atagttaaat
     9841 aatcaaaatt taagagagtt tatttgcaaa ccttacatag atgtaaggtt tgtgtaatgt
     9901 ttgcagatag ttaaattttc taatctaaat tacaattaat ataagaaata acataagaga
     9961 aataacataa aaaaagaaat tttgtaaaaa aggaaggatg tttattgatg agtttagatg
    10021 ttatttttat attgaaatca gtcataatag ctatagtaga ggggcttaca gaatttattc
    10081 cagtttcatc tacaggtcat atgattttgg ttggtaattt aatagatttt aaggggcaat
    10141 ttgctgaaat gtttgaggtt gtaatacaac ttggggctat attagcagta gtagtacttt
    10201 attggaaaaa gataaaagat agcgttatag aattttttaa atttatattt actggtggaa
    10261 aagaaggaaa gattggtttt agatttggaa tgaatgtaat tataggttgt ataccatttg
    10321 cgattatagg agttctattt tacgataata taaaatcatt gtttaattta caatctgtaa
    10381 ttataggatt tatagtgggt gggattttat tattagtagt agaaactttg tttagaaaaa
    10441 agaatcattc cacagataat atagataaaa ttactccaat acaagcttta aaggttggga
    10501 cattacaagt tttgtctgca tggccaggta tgtcaaggag tgcatctact attatgggtg
    10561 gatggatagc aggacttaat tctcctacag cagctgagtt ttctttcttt ttagcagtac
    10621 cagctatggt ggcttcttca ggaaaagatt tatttgaatt tgattattca ataatgacac
    10681 caacattgtg gattgctttg gtagttgggt ttatagtagc ttttattgta tcaataatag
    10741 ttatggaaaa attcgtgaat tttttaaaga aaaaaccaat gagagtgttt gctgtataca
    10801 gaataatcat gggtgtagta cttgcagtat tagcttttac taacataatt agtgtataga
    10861 aaaaatagat ttattatcta taaaaattaa actaagataa attataatta acaattatat
    10921 atatgttatt tcaaaaatta aattaggaag gatagttaaa taatgaaaat tttaactgta
    10981 aataatttaa gtaaagtata tggtaaaaaa ataattttca acgcattaaa tgacataaat
    11041 tttagtatag aagatggaga atttgtagga attatgggtc catctggtag tggaaaaact
    11101 actcttttaa atatgatttc tactattgac aagccaacca caggaactat ggaattaaag
    11161 ggcaagaatc ctttattgtt aagaggtgag gaacttgcat tatttagaag aagagagtta
    11221 ggttttgtat ttcaggattt taatttattg gatacattaa ctataggaga aaatatagta
    11281 ttgccattga cattagataa ggtcagtgta aaagagcaag atgagagatt gaatgaagta
    11341 tctacaatat taggtataaa agatttatta ggaaaaagaa cttttgaagt atcaggtggt
    11401 caagctcaga ggactgctat tgctagagca ttgattaaca atccatcaat acttttagca
    11461 gatgaaccaa caggtaattt agattcaaag tcatcaaagg ttgtaatgga gctatttcaa
    11521 aagataaata aagaaaataa agttactact atgatggtaa ctcatgaccc attagcagcg
    11581 agctactgta gtagaatctt attcataaaa gatgggtcta tatataatga aatatataag
    11641 ggtagtagta gagagcagtt ttatcaagag ataatggatg tactcacatt attgggaggc
    11701 gataactaat gaatataaaa cagtttgcta ttaacaatat ttcaagaaat attaaagcgt
    11761 atttaggata cttagcaagt attgttatat catcttcact gctattttct tttattatgt
    11821 ttacaagtca tccagattta gatgtatctc aatttcctga ttatttaaaa gaaggtctca
    11881 agatgagtaa gataattgca tatctattct tattcttctt tgtgttttat tcagttagtg
    11941 tgtttcttaa aagtagatat aaagaatttg gaatacttta tattacaggt atatcaaagc
    12001 gccaagtaat gaaattaatc tttatagaaa atatgataat aaatatagta tcttctgtaa
    12061 ttggaataat aataggctta atctttgcaa agatattttt agtggttatg tctacttttt
    12121 tagaattatc gccattaaaa ttctatatac catttaatgc gatattgtct actttaatct
    12181 attttatgat tttagctgta ttaacatcaa tatttacttc ttttatagtt aaagaaaatc
    12241 aggtgttgaa gttacttaaa ggtacaaaaa ctccaaaacc agagcctaaa acatcactaa
    12301 tgcttgctat attaagtatt tgtttgttta taatagcgta ttataatgct attacctcaa
    12361 ccgaccaata tacaacatat ctgcgcttag ttccagttac atcccttact atagtagcaa
    12421 cttacttgtt cttttctcaa tttagtgtat tttttatgaa gaagctaaaa ttaaacaaga
    12481 gattttatag aaaaaataca aatatgcttt ggatttcgaa cttgatttat aaggtaaagg
    12541 ataatgctag aatatttttc ttgataacta taacttctgc agtggcattt actgctatag
    12601 gtacagttta ttccttttgg aaagatgtag agcgtcaaat taatttaata tatcctaaca
    12661 caatatatta ttcaacaatg acattgcata atgacactaa aaagcctgat agcaaagaaa
    12721 aagacaagga aagaatgatg tttatagaaa ataatctaaa aaaagagaat gtagactata
    12781 caaggattaa tggtgaattt aaaactgtat ttcccaagaa aagtgatttt aatgtcagaa
    12841 ttatgaaaga atctaagtac ttagaaatta cagaaactat agggattaag cctataagtt
    12901 ttgaagataa tgaatgtata tcattattat catcaagttt accaggaaat aagaacgtca
    12961 aagaaaatat tattgtaggg aatagaggtt taaaggtggc aaaacaagtg gaagaatgtg
    13021 ttatgccagc atattacaaa aatatgtatg tggtaaagga taatttttat gatagtatag
    13081 aatcaaaatt tatagtggat agatttactg catttgaagt agaggattca agtaaaacta
    13141 tagagatatg cagagaattt gaaaataaat ttaataatga gtcagggatg caaccatatt
    13201 tgtttttctc taaagaatta atgataggaa ctgggaaatt aatgtattct acatttatat
    13261 ttttagcaat atttataggt ttaattttct ttgttaccac aagtagtttt ttatataata
    13321 aattttatat ggattgccaa gttgataaga aaaagtatga acagttaaat aaattgggta
    13381 tgacatataa tgagattaaa aaagcttcaa caattgagat tggaatagta tttttgtttc
    13441 cttatgtagt ggctgttata cattcaatat ttgcacttac tgcacttaaa aattcttttg
    13501 atatagaagt agcatcatca gctgttttgg taatgggaag cttgttcttg gttcaaatag
    13561 tttatttttt aattgtaaga aataattatt taaaagagat aaggttaaat ttagtcaatt
    13621 tttagttact attattaaat gataataaaa agggtttaaa attaattaat attactggaa
    13681 ttata
//
LOCUS       DAMJYC010000104        13258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJYC010000104 DAMJYC010000000
VERSION     DAMJYC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.108"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            155..1573
                     /locus_tag="PC472_003222"
     CDS             155..1573
                     /locus_tag="PC472_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2728526.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1649..2461
                     /locus_tag="PC472_003223"
     CDS             1649..2461
                     /locus_tag="PC472_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728527.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            2884..3501
                     /locus_tag="PC472_003224"
     CDS             2884..3501
                     /locus_tag="PC472_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2728528.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3658..4110)
                     /locus_tag="PC472_003225"
     CDS             complement(3658..4110)
                     /locus_tag="PC472_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2728529.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4558..5916
                     /locus_tag="PC472_003226"
     CDS             4558..5916
                     /locus_tag="PC472_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2728530.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6189..6569
                     /locus_tag="PC472_003227"
     CDS             6189..6569
                     /locus_tag="PC472_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2728531.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6694..6894
                     /locus_tag="PC472_003228"
     CDS             6694..6894
                     /locus_tag="PC472_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728532.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7090..7818
                     /locus_tag="PC472_003229"
     CDS             7090..7818
                     /locus_tag="PC472_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2728533.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7840..8757
                     /locus_tag="PC472_003230"
     CDS             7840..8757
                     /locus_tag="PC472_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2728534.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTKEEAEQQILNCFLK"
     gene            8899..10083
                     /locus_tag="PC472_003231"
     CDS             8899..10083
                     /locus_tag="PC472_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2728535.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10190..10378
                     /locus_tag="PC472_003232"
     CDS             10190..10378
                     /locus_tag="PC472_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2728536.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10527..11177
                     /locus_tag="PC472_003233"
     CDS             10527..11177
                     /locus_tag="PC472_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2728537.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            11280..12218
                     /locus_tag="PC472_003234"
     CDS             11280..12218
                     /locus_tag="PC472_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2728538.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12244..13095
                     /locus_tag="PC472_003235"
     CDS             12244..13095
                     /locus_tag="PC472_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2728539.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5291 a         1277 c         2341 g         4349 t
ORIGIN      
        1 taagtataac actcacattt taatgtattg tgaaattttt gttgaacttt atgtaccata
       61 acttataaaa tttatgtcat gttatatttg aataagtaat aaaattgtag atgtaaatta
      121 atctacaaaa tttatatgaa atggagttga tgctatgaca gaattaatga ttataagtgg
      181 gttagtacta ttgatttgta taacttctag caaggtattg tataaatttg gagtaccaat
      241 attattgatt tttatcttac tagggatgtt atttgggtct gatgggatgg taggaatcta
      301 ttttgataac tatgaactga caaaacaatt atgttctatt ggactaatat ttataatgtt
      361 ttttggaggg tttggaacaa actggaatat ggctaaacct gttgctatac cttctatatt
      421 aatgtcttca ctgggtgtaa taattacggc tggggttaca ggtatgtttt gccacatggt
      481 aataggaact agtttacttg aaggtttatt aattggggca attgcagctt caacagatgc
      541 tgcatctgtg tttgctgtgt tgcgttctca aaaactaaac ttaaagggtt cacttgcatc
      601 attattagaa gttgaaagtg gaagtaatga cccaatagcg tatatgctga cactaattct
      661 tttgacattg atgaataatt ctggggtaga acttattgca ccaatggtaa taaaacaaat
      721 agcttttggg ttagctatag gatttatact agcaaaatta tctgtttata ttttaagaca
      781 ttcaaacttc gagatagaag gattttatac tatatttata acagctattg caatactttc
      841 ttatgcattt agcgagtatc ttggaggaaa tggatattta agtgtttaca tttcaggtat
      901 tattatagga aatagtagga tacctcttaa aaaaagtttg gttcatttct ttgatggaat
      961 ttcatggatt atgcaaataa tgttattctt tatgttagga ttgttatctt tcccttctaa
     1021 gttaccaaat gtatttggta tagcaatggc catatctata ttcatgatag ttattgcaag
     1081 accattagca acatttatag ttctagcaaa atttaaattc tcaaataaag aaaaattatt
     1141 catatcgtgg gtaggtctta gaggtgcggc ttcagtagtt tttgcaattt atgctgttac
     1201 tcaaggagta gccatagaga atgacatatt ccatataata ttttttatag cgttgctttc
     1261 agtaggaata caaggttcat tgataccaac tgtagccaga aagttggatt tagttgatga
     1321 taatacacct gtattaaaaa catttaatga ttatgatgga gaaattagct ctaaacttat
     1381 cgaagtaaat gttgatgagg gtaataaatg ggctaataag agtataatag attctaatat
     1441 accagacgat atattaatag ttatgataaa aagaaaggaa caggttttaa tacctaaagg
     1501 ttcaaccatt ataaaaacag gggatatttt ggtattgagt ggagatagaa ttgaagaatt
     1561 aatacaatta taatttattt tgtattgact ttaacctaag gggaaagttt aaacttaaat
     1621 taatcaaatc taatgcgcag gtgatgagat gttttctata ggaatgtttt ctaaaataaa
     1681 taaaataact acaaagacat taaggtatta tgaagatata gggttgttaa aaccagaata
     1741 tgtagatgaa tttacaaaat ataggtatta tacaacagag caattaccta aattacatca
     1801 gataataact ttaaaacaaa tgggtttatc attagctgag ataaaaaaag ctgtagaaaa
     1861 tcctgaagaa gttgaaaata ttttaatgaa taaagaaaaa gaaatgttgg atgtaattaa
     1921 aagtgaagaa tataaaataa ttaaactacg taattacctt atgaatttga aaggagaagt
     1981 caatatgaat gatgtaatta ttaagtcttt accaaaagtt aaagttgctt ctatgagaaa
     2041 agtgttaaag gggtatgatg aactttttaa tctatttcca aatgtaatgg ctgaagaaat
     2101 gatgaaaaca gggtgtacat gtgcagttcc tgattattgt tttaatatat atcatgatgg
     2161 tgaatataga gagactgata tggatgttga aatgtgtcaa gctataaatg atttaaagga
     2221 agatacggat atattaaaat ttaaagaatt agatgcagtt gaaaatgcag tttgtatatt
     2281 acacaatggt ccatattcaa cattaggaaa aacttacgca gatgcattta aatggataga
     2341 ggataataaa tataaagtgg ttggaaatcc tagagaatct tatatagatg gtatatggaa
     2401 taaagagagt gaagaagact ggttgacaga agttcagata ccagtgaaac gcatgttata
     2461 atatgtctac aaagtataaa taagaataaa aaaggtggtg tctcaaagta aacattttag
     2521 tttatgagac actctttttc atatgaaatc aagtatattt ttataattac gattatgaaa
     2581 aaaggcttat ctatattttt agacagcctc ttattttttt attataatag ttttctaaag
     2641 atgctctatt tataaatttt tattaaagac tttataaata attgtattta atatacaaaa
     2701 tatatataaa tattgatgtt ttatatataa agttgtatat ttttttaagg atataaagta
     2761 aaaaaatata caatatcaag aaaaaaagat attgggtatt taaagtaata ctttatttaa
     2821 ttttaagtaa atcgggtatc tacatatata gatactaatt tcaaagaata ggagggtgtt
     2881 tatatggaaa tattcgcaat aggacattca aattatccat atgataagct tattaatatg
     2941 ataaaaaaat atgacataaa ttgtgtagtt gacattagag aaactccata ttctaaatat
     3001 aatattcaat acaataaaga ggcatttaac gaatctctta gaaattccgg atttttatat
     3061 atatacatgg gaaaagaatt tggagctaaa agaactaata aagatgtata tacgcaagaa
     3121 ggttatgcag attttgagaa agtagcaaaa gaagatattt ttttaaacgg aatagaaaga
     3181 ttaaaaaagg gttgtcaaat gggttatagg atagttttat taggagctat gcaagaacct
     3241 ataagatgtc atagaagtat cttggtggga aaagtattaa ataaagaagg ttttgatgta
     3301 aaatatataa tgcatgaagg aaatttggca tatcaggaag atatagagga atcattattg
     3361 gataagtatt ttagtgatag aaagcaactg agtattgaca atttattagg gagtgcatta
     3421 acgagagaag aaatgataca agaaggttat aagctagcta ataaagaaat aggatataga
     3481 actgagaaac taggtaaata aaaatataca aatagataaa atacagtact tatcttaagt
     3541 aaaataatat tatgaattta taatagctag gcatgatgtg ccaattaaaa atattctagt
     3601 tatttaataa tatcattaaa tttagggtat tttttaatag cacatcatgt tgcagtttta
     3661 aaaaataaaa tctctaatat tgccataatc aactgttcta ttaagagtag gatgactaga
     3721 tataaatcta actgtaattt ttgaatttaa caaacaagga tttgaaatta aaaatactct
     3781 atctccattg acaatagaag taatagctac tggcaaaata tctacaaatg tatcatatct
     3841 gtttttagca atttgataag gataatttcc tacaaaaaca aatagattta aatcatatat
     3901 attatcagta aaccaagccc attgaccata aactttgtca atattcattg tgcctaaacc
     3961 aagtgtatca aagtatgttg ctatagttaa ataaatattt tccatgtttt ctaaatatga
     4021 aacagtatat cttctgaaac gtatgggtgc tgtggaagaa ataggataaa ccatatttac
     4081 atttagatta tttgcattta aattaaccaa agtatcacat cctaaaatta gtctataaaa
     4141 tacaatattt aaatggatgc gattttgtta catctaaagt gtaatatttt atatattgaa
     4201 aaagtggtaa aagtttggat gattaaataa tattataaaa aatatataaa atgtacttat
     4261 tgttattgct acacgatagt gatacaatat taacgaaata tagctacttt taataataac
     4321 ggaaaatatt tatattaaat taataaatat tttaaatgtg gtatccataa aaaataataa
     4381 acataagata attataaaga tatactgtgt tcaaatgata ttaaacaaga tttatattat
     4441 ttgaaagcag tatattataa tgcgaacaat acaattctaa actttgcatt gtcaatttac
     4501 agttcgataa actaattaaa aattaaattt atataaagag ggaaaagagg aatagggatg
     4561 tttataagga aaaatgttat aaagttagca attcctataa tgatagagca gacgtttgtc
     4621 atgcttttag gagtatttaa tacaatgatg gcaggtcata ttggggaaga agctgtatct
     4681 gcagttggaa tggttgattc aataaacaat atatttatat ctgtatttgc tgcactatct
     4741 gttggagcta ctgttgttgt agctcaacaa ataggaaaaa aagatagtga aaaggtgaat
     4801 gaaactatta agcaggctct agtgtctgga gtaatattat cctttacaat aacaatattg
     4861 atgtgggttt ttagaactgg tgtaattaat ttattttatg gttccgcaga agaattggta
     4921 aaatcaaatg caaaattata catagagttt acattattta cgtatccatt tattgcgaca
     4981 caacaaatag caaatggtat tttaagaggg tgtggagatg taaagaaacc tatgtatgtg
     5041 actatgttta tgaatatagt aaatgtaaca ttgggatata tacttatata tggtattgat
     5101 ttaaatcctc taggaataaa tttgcaaaca ccatcctatg gcataacagg tgcagctata
     5161 tctattgcta tagctagaat aattggatgt atagtaatta taagagcttt atttaaagga
     5221 aacgaattta tatctatggg taaaatattt ccatttaaga ttgatgttga aactcaaaag
     5281 tctatattta atataggaat accagctgga gttgaacaac tactttttaa tgcaggtaaa
     5341 ttgattgttc aagtatttat agttactatg ggaacatcag caattgcatc caatgcaata
     5401 ggaatgtcaa ttgcatccat aataaatgta attgggaatg ctttagcttt gtctgctaca
     5461 accttggtgg gacagtatat aggacgtgga gatattaaag gagctaagag tactttatta
     5521 tatttaacta aatttgcaac aatttgttta tttatagtag gagctatatt tataccaatt
     5581 gcaccctgga tttcaagtat gtatactcaa aatgctagtg taattgacat ctcaacacaa
     5641 ttaattcgaa gtgattgttt agctatggta atatggccta tatcctttgt attatcagca
     5701 gggctaaagg gagccggtga tactagatat acaatgatga ctgcagtaat aggtatgtgg
     5761 atttttagaa tttttacagg ttacgtattg ggggttgtat tagaatttgg agtgttagga
     5821 atttggatag gtatgtatac tgattggctt gttagaggga ctttatattg tttaagatta
     5881 agaggaacaa agtggctaaa acacaaagtt gcatagaata tatgaaatca cttttagatg
     5941 gataactcta tttagaagtg attttttatg tagttattgt cataattata agtatgatat
     6001 ataaggtgat taaattacat aaataaaggc tataatagta tggatattat aaattatata
     6061 ttgagcagaa ttacataatt tggcgataaa tattagaaat atatatctat agaaaataat
     6121 atatatgggt atattattaa atataaaaaa tattaggtat ttatttaata ataaaggtgg
     6181 gtattgttat gataaataaa ataggaaaaa taactctcta tgtaaataat caagaggatg
     6241 caaagttatt ttggacagaa aaattgaact ttgtagtaac ttatgaaaag gaaatgggtc
     6301 caaatatgaa atgggtggaa gtagcaccaa gtaaaaatga atttacaaca tttgtgttgt
     6361 atgataaaaa tctaatgatg aagcaaaaca aagagataaa tgtatctcat ccatcaataa
     6421 ttttaagtac aagagatatt aatggaactt atgaacaaat ggcaaataac gaggttgagg
     6481 ttggggaatt aatggagatg ccatatggaa ggatgttttc ttttaaagac caagatggaa
     6541 atgattattt ggttagagag gataaataat tttacaaagt attaaattta ctcaatatct
     6601 gatataatta gagacaataa ttattataaa ctagtctagg attagaatta tataattaaa
     6661 aattaagtgt aaattttgag tagaggtgat aaaatgttta agaaaatggc agtactaaaa
     6721 gatatagcaa ctaaaatagg tcgtaaaaaa gcatatgaac tattagaaat ggttgaaggt
     6781 aatgatgcct ttgtagctga ggtaaagata aaaaagaatg gaatagaatc taaaaaagaa
     6841 gaaattatgt taaaagataa tcaaaaaata atattagagt atatagaagg ttaaatttta
     6901 aatacatata agttgatata ctaaatttct gagatgagtt tacataaaac tcatcttttt
     6961 tacttttcaa taactttgtt ttattattta atatacattt tatctcctta gagttgctga
     7021 tatataaaaa ttactgatat aataaaagaa tggatattaa tgtaatattt tatagaaaag
     7081 aggggtatta tgcttgaaat aaaaaatctt tcctttaatg ttgagtctaa caatgaggaa
     7141 ctaggaataa taaatgatgt tagcctttca tttgagaggg gaaaacttat tgttatcaca
     7201 ggtccaaatg gtggaggaaa atctacaatt gctaagctta ttatgggaat tgaaaaagct
     7261 acgagtggtc agataatttt agatggagaa gatataacaa atcttagtat tacagaaaga
     7321 gcaaaaaaag gaataggata cgcatttcaa caaccaccaa gaataaaagg tatgacagtt
     7381 gaaaatttac ttacacttgc tcatggaaaa ccattgtcta ctgatgtatg ttgccaatac
     7441 cttacagatg ttggattgtg ttcaaaagac tatttaaatc gtgaagttga taactctctt
     7501 tctggagggg aaatgaaaag aattgagata gcaactcttt ttgctagaga tttaaaggtt
     7561 tctatatttg atgaacctga agcagggata gacctttgga gctttggaaa actcaatgaa
     7621 agttttaaga aaatacatga agagtctaat cagactataa tcataatttc tcaccaagaa
     7681 agaatattag agttagctga tgaaataatt gttctacaag atggttcagt aaaaagccat
     7741 ggaacaaaag aggctatatt acctgaaatc atgtgtcaag ttaattcaag ttgtgaatta
     7801 atgaaagaca tgaattaatt agaaaggaag gcaaattaaa tgaacaaaat agatttaaat
     7861 cttttagaaa aagttgctga acttcgtgga actccattag gagcatacaa tatacgtaaa
     7921 aatgggcaag gagtagagag acattcaact gaaaatgtaa ctataattcc taaaactgat
     7981 aaacctggaa tagatattat agtaaagcca aatacaaaag gagaaaatgt acatatacca
     8041 gtaatagtaa cagaggctgg tgtacaggat atggtatata atgattttga aattggagaa
     8101 aatgcagatg ttgttataat tgctgggtgt ggtatacata actgtggttg tgatgatagt
     8161 gaacataatg gtatacatag atttttctta cgtaaaggag ctcgcctaaa atatgtagaa
     8221 aaacattatg gtgaaggtga tggagaagga aagagaattt taaatccagt tactattgtc
     8281 aatatggatg aggattcata ttgtgaaatg gaaacaattc aaattaaggg tgtagattca
     8341 actaaaagag aaatggatgc aaacctaggt aaaggagcta aaatagttgt atttgagaaa
     8401 ttattaactc atggtaatca agttgcagaa tctaatatga aggttatctt agagggtgaa
     8461 aactcttctg ctcaagtagt ttctcgtaca gttgggcaag ataattctaa gcaaaagttt
     8521 aatccttgtg ttattgggga ttccctttgt tctgcacatg ttcaatgtga ttcaataatt
     8581 atgggaaatt ctcaaattag tgctatacct gaaattgctg caaatcatcc agatgctctt
     8641 ttagtacatg aagcagctat tggaaagatt gcaggagacc aaatactaaa gatgatgaca
     8701 ttaggtctta ctaaagaaga agccgagcaa caaatactta attgcttctt aaagtaaatt
     8761 aaagatttta tttaatttaa tataattaat atagtataat atagtgaata agtctaaaat
     8821 atttttattt ttaggcttat tttctttatg taagaaaagc tattagagaa attatattgt
     8881 aattaggatg tggaataaat gaaaaattta cttataagaa atgggacaat aatatctcca
     8941 acagatggta tcaaatttga aaaaagagat atcttggttt tacatgggaa aatatatgct
     9001 attggattga accttaaaga gaaaataaga aaagaaaaat tacttacaaa taaagagttc
     9061 gagataataa atgccgataa tatgtatatt tccccaggat ttatagatgt tcacacccat
     9121 tgttataaga gaaaattacc tacaggtatg gattctgatt caattggaat tgaaaaaggt
     9181 tctacagtta tatttgatgc aggtacagct ggtccctcaa actactatga ttttaaaaaa
     9241 aagtatatgg atgagtgtaa aacagaagta tattcttttt taaatgttac aaatgaggga
     9301 ctaaatatat tgaatgaatc aacttattta gatgttataa attttgacaa ggttgagtca
     9361 attgtagaaa aaaatataga aaagataaag ggtataaaag taaaagcgag taaatttcaa
     9421 tctaatgaaa gtggtataga tataattaag aaatcaaagc aagttgctaa aaaattaaat
     9481 ttacccttag tggtatatat aggagagtat ccatcatata tagatgaagt aatgaatatt
     9541 ttgggaaaag gagatgtggt tacaccggta tatggaaata gtacaaatgg aatccttaaa
     9601 gaatctggca cattaagaaa atcagtaata aatgcaaaga atagaggagt attgtttgat
     9661 gtatgtcatg gagtggacca atttgatttt aaagttttta aaaaagcaat aaagcaaggt
     9721 ttagagcctg acctaataag tactgatatg catataaaaa acatgaaaga cgtaaactat
     9781 tctcttttaa atgttataaa taaaattatg gaacttggga tatccctaga aaagtgtata
     9841 gaaaaagtaa ctgaataccc aacaaagatt ttcgatttac atggaaataa aggcagaata
     9901 aagattggtg gagaaggtga ttttacaata tttacaatgg aggagtgtaa tgatattata
     9961 aaagactaca ataataatga acttgtttta aataaaaaat taagattgca atatactgta
    10021 aaatcatgga tgaaaagcag tgaagtttat agacatggat acgaaaatac tataataaac
    10081 taaacaatag tgataaaata ataaatatat gtttaaattg gtatatattg gataaatatc
    10141 taaggaaaca acaattaaaa aataataatt ttaggggagg tattttagta tgaaagcatt
    10201 tgttgataag gatgtatgtg tagcttgtgg agcatgtata ggaacatgtc cagacgtttt
    10261 tagtatgagc gatgaagggc attcagtagc aattgaagga gaaataccag cagatttaca
    10321 agatttatgt aaagaagccg aagaaggatg tccagtttca gctataacag ttaaatagag
    10381 ctgtttaaat ttatttttaa aataaatata taagatttaa ttggtactta agctatagta
    10441 ttagaaattt ctattactat agctttttaa aatagataaa cttaatatat aagatattac
    10501 tttttacaaa tatggaggac tgaaatatga aaaaaaatta tgaaatagta ttattcgatt
    10561 tagatggaac acttacagac cctaaagaag gaataacaaa gtctattcag tattctttaa
    10621 atagctttgg cataaaagaa gatttggaaa acttagacca atttatagga ccaccattac
    10681 atgatacttt caaggagtat tataaatttg aagataaaaa agctaaagaa gctgttgaaa
    10741 agtatagaga atattttgct gataaaggga tatttgaaaa taaaatttat gagaatatga
    10801 aagagatatt agaaatgctc tataaaaatg gtaaaatatt attagtagca acttctaagc
    10861 caacagtttt tgcagaaact atacttagat attttgatat agatagatat tttaaatata
    10921 tagcaggtag caatcttgat ggtacaagag tgaataaaaa tgaagttata caatatgtac
    10981 tagacctatg taatgtaaaa gataaagata aagttattat ggttggagat agaaagtatg
    11041 acataattgg agctaaaaaa ataggtatag attctattgg agtattgtat gggtatggtt
    11101 cttttgaaga gattagtgaa agtgaaccta cttatattgt cgaaaatgta gagtctataa
    11161 aagatatttt gttataaata tcttttatag acaaaaatat tacaaaatgg tgtcgcatat
    11221 ttaaaaatat gatattatag ttaaggaagt catttaaaaa agaggaggat gcacttaaaa
    11281 tgaaatctat tttaattatg ggtggaagcg acttcatagg ttctgcactt gctaagcgct
    11341 tgattaaatg tgggtatcaa attgatatac ttacaaatgg taaaaaggaa atagattata
    11401 atggttttaa agaacatcta atttgtgata gaaaagttcg aaaagatatg gaaaacatta
    11461 tcacaggaag aaaatatgat tacatatatg atatgacagc atatacaaaa gaagatgtaa
    11521 gtaatctgat tgattttata agcatggata acttaaaaaa gtatatagta ttgtcagcag
    11581 gagcagtata taaagatagt ggaagaaata taaaagaaga aaatgagaaa ggtgaaaatg
    11641 aaaactgggg aaaatatggg ttaaataaaa aagaggctga agattttatt ataaatagcc
    11701 ctataccata tataattatt agaccaactt acatatatgg tgaaaataat aatttatata
    11761 gagagtatta tttctttgaa aaaatagaaa aaaatgaaaa gataccagta ccaaaaggaa
    11821 aacaagttag taatcaattt atatatattg gggatttggt aaaggtattg gaaagcataa
    11881 tgaaaaatcc tcatgtaaga gaggcctata atgtgacaaa tccacagtta atttcttggg
    11941 atgaccttat atatacatgt ggagaaatta ttggcaaaga acctataata aaatatgtag
    12001 atatggaaaa agtagagttt agagaaagaa cgtatttccc atttagaaat atagacttta
    12061 atcttgatat aaataagctt attgaacatg ggctttatat accaaacgtg ttactaaaag
    12121 aaggtcttac agcaacatat aaatggtttt cagcaaataa gccaaaaatg catgacagaa
    12181 aaatgaacaa agttgagtct gtaatgcaaa tagtttaaaa aaataaataa gtaggtagat
    12241 attttgaact caaaaattaa gtatttttta aaatttttta tcttaatatt tattttgatt
    12301 tttatatact cagttgcgat agagccgagt ctattaattg tgaaaaaata taatataagt
    12361 gaaaatggag cattgataaa aaatgattca attgagagta aatctgaaag tgtaaaagta
    12421 gttcaaattt cagatactca aataggtagt ttttattcaa ctaagaatct caaaaaagta
    12481 gctaataaga ttaacacttt gaatccagat ataatagtat ttactggtga tttaattgat
    12541 tactccaata aaaatccaag tgtagatgaa attacaacta ttttatcaag tatgaatgct
    12601 aggttgggaa aattttcagt atttggcaat catgattata tgtataaact tcctaggtat
    12661 tataggcaga ttatgaaaaa ttccaacttc aatcttttag taaatgaaaa taaaaaaata
    12721 aaattaaaag atgataaata tattaatata ttaggtgttg atgaaatatt aaatggaaat
    12781 cctaatataa aatatctgga gagtcaaata gataataaaa gttttaattt attgttagca
    12841 catgaaccag atttagtaga tatgcttagt aaagatacaa tgaatttagt tctatctggg
    12901 catagtcatg gtggtcaaat aagattacct attaagggag cattagttac tcctccatat
    12961 ggaaggaaat atactaaagg gttttatgac ataaatggaa atcatttata tgtgagttcg
    13021 ggtcttggaa gtacaaagtt accatttaga ttttttaata taccagagat aatagaattt
    13081 aatattataa tctaacataa aatatttaga aaatataatt ttgcttttta taatatgtag
    13141 atgtatatac tgtaaaaatg gattaaacca gtagattgaa tgaattaata gattaaatta
    13201 atatataaaa tatttaagtt ataattaatg tattagacaa aataaatact attaagaa
//
LOCUS       DAMJYC010000105        13220 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJYC010000105 DAMJYC010000000
VERSION     DAMJYC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.109"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(78..2078)
                     /locus_tag="PC472_003236"
     CDS             complement(78..2078)
                     /locus_tag="PC472_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2728540.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2109..3827)
                     /locus_tag="PC472_003237"
     CDS             complement(2109..3827)
                     /locus_tag="PC472_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2728541.1"
                     /translation="MNFRLPKKRLPKKIIFICIVLISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4053..5291)
                     /gene="fabF"
                     /locus_tag="PC472_003238"
     CDS             complement(4053..5291)
                     /gene="fabF"
                     /locus_tag="PC472_003238"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2728542.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5320..5544)
                     /gene="acpP"
                     /locus_tag="PC472_003239"
     CDS             complement(5320..5544)
                     /gene="acpP"
                     /locus_tag="PC472_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2728543.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5598..6347)
                     /gene="fabG"
                     /locus_tag="PC472_003240"
     CDS             complement(5598..6347)
                     /gene="fabG"
                     /locus_tag="PC472_003240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2728544.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILINNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6347..7297)
                     /gene="fabD"
                     /locus_tag="PC472_003241"
     CDS             complement(6347..7297)
                     /gene="fabD"
                     /locus_tag="PC472_003241"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2728545.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDVLNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7313..8242)
                     /gene="fabK"
                     /locus_tag="PC472_003242"
     CDS             complement(7313..8242)
                     /gene="fabK"
                     /locus_tag="PC472_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2728546.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8351..9337)
                     /locus_tag="PC472_003243"
     CDS             complement(8351..9337)
                     /locus_tag="PC472_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2728547.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9363..10385)
                     /gene="plsX"
                     /locus_tag="PC472_003244"
     CDS             complement(9363..10385)
                     /gene="plsX"
                     /locus_tag="PC472_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2728548.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10396..10953)
                     /gene="fapR"
                     /locus_tag="PC472_003245"
     CDS             complement(10396..10953)
                     /gene="fapR"
                     /locus_tag="PC472_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2728549.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11160..11336)
                     /gene="rpmF"
                     /locus_tag="PC472_003246"
     CDS             complement(11160..11336)
                     /gene="rpmF"
                     /locus_tag="PC472_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2728550.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11361..11885)
                     /locus_tag="PC472_003247"
     CDS             complement(11361..11885)
                     /locus_tag="PC472_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2728551.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(11978..13174)
                     /locus_tag="PC472_003248"
     CDS             complement(11978..13174)
                     /locus_tag="PC472_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2728552.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3887 a         2610 c         1376 g         5347 t
ORIGIN      
        1 tctctataaa tataaaagtt tattattatt taaatatcta aattcaaaat atgtgatttc
       61 aatatcaaat acttagctta atttatatta ttataaagtt ttatcccctc tgaggttatt
      121 tgacttcctc ctctaccaga tttagatgat atgtatccac attcacttaa ataagatagc
      181 acctttctaa ctttgctttc agaaatctca ataccttcct gtataagctt ttcgattata
      241 aaccctcttc caattccaga ggatgactct cttctttcat atagaatcct cagtatttcc
      301 tcaaattctt ttcttgtata accaaatcca tcttcataat tgttagcaca ttctaccctt
      361 atatatggtg gtaagtcatc tctaaaaacc atactatcac acattagact tacataagaa
      421 gcaacatttt gtaactctct tatatttcct ggccaattat atttttctaa aatagttata
      481 acttctggcg aaagtcttat attgtttttc aaaaagaaat tcataagtgt tattatatct
      541 tcttttctat tcctcaatga tggtacatct attggcaata catttagtct ataatacagg
      601 tcctctctaa attttccttc tctaatcatc tttagtaagt ttttgtttgt tgctgctatg
      661 accctaacat ctatgtttat aacattatgg gaacctatag gcataacctg cttttcttgt
      721 aataccctta gtaactttgt ctgtagcaat ggaggcatat ctccaatttc atccagaaat
      781 atagttccat tattagcaag ttcaaataga cccttttttc cttctcttaa agctcctgta
      841 aaagagcctt tttcatatcc aaacagttga ctttctaata gactatctgg aactgctgca
      901 caattaactg ctataaacgg ttgattactt ctattggaag cattatgaat tgattgtgat
      961 aataattctt taccagtacc actttctcct attattaata cagaaaggtc tgatttagat
     1021 atttttttag ccaattctac acactttacc atatttttat ttttagtttt tatatcttta
     1081 aatgtatatc ttgctatctg accttgttct cttaatttat tagtaagatt ttgttctaat
     1141 ttttttatat acgttatttc ttgtaagtta taatatatcc cagctttatt ccccatagaa
     1201 aaaatacttt ttttatttac atttatatat tttttatttg taaataccac ttcatcaatt
     1261 atttctttgt cttcaagtaa aatatttaaa tcttcagaaa tgatatcttc tatgtttttg
     1321 ccttgtggat tttcatttat acctaaaatt tttaaagctt ctttattaaa tgtgtttatt
     1381 ataccatctg ctgaagtaaa tattattcca tcttttgata gattcaatat aacatcttgt
     1441 tcttctattt ttaaaaacag ttgcctataa ttatctttaa ctccattatc taaacttatt
     1501 aaactctcag aatatgccat taaattttta cgtatttctt taacatctat tctaagacca
     1561 tttattattt ctataaaggt agatatatca atatatctat ttccaaggtc gattacttta
     1621 tttatataac ttggtacttt tccctttact ccaggtgtta tagctatgtc tatattttta
     1681 taaatttttc cttcaatata tggagtaaac tttaaatttt ttactcctat tttgtaaaat
     1741 aagcttatcg tttctaaggt agtttctttt gtatcattta caaccaatac ttcagttcca
     1801 tttggtattg aaaataatcc atatatttca ttttctttaa gtgttctcct tatcactata
     1861 atcttactat cttgtactaa atgctttctc atactgttta atctatcatc attcattact
     1921 agtacaaaat caccttgtat tttatcattc tcttttaaaa cttccagata atagtggttt
     1981 atatcaatat aattacctaa aaccaaacgt atattttctt ctaagaaaga tgatagttta
     2041 gttattttat cagttactat agctattgat tgtttcatat ttatacctct tatttatata
     2101 gtatataatt acttatatac tactttattt ctaccaagtt gttttgcttt atataaattt
     2161 ttatcagctt cttctagtac atctttgcta tctgaaacat gagctacacc tgcacttata
     2221 gtcacagtca catcatcatt ccacttttta tttgcaattg caacccttat actgtcaatt
     2281 atatctgcta cttcatcacc agatttattt cttattaata cagaaaattc ttctcctccg
     2341 tacctaaaga ctttatcatc ttgttctaac ataacacaaa tggtatttgt tattcccttt
     2401 ataacagtgt caccaaattt atgtccataa ttatcattta tgagcttgaa attatcaata
     2461 tctatcatca caagtgcaaa attatcatct ttctctctgt acttttcata cataatatca
     2521 aaactttttc tattataaac atttgtaaga ccatcatgta aattttgtgc tttaaaatac
     2581 ttcaatcggt tgtataaaaa agtagaccct aatatagcaa ttaaaattac tgtaaataat
     2641 attttataat attttatttc ttttttagtt ttttcaaata gcttactttc gtttgatact
     2701 ttatcaatta tatagaaact agtctctgaa tctcttatac ttttgttctc tttatattca
     2761 tttattaaca tcttttgata ctcatttaaa gattctatat ttccttgttt ttcatatata
     2821 tttaatatct tatttaaatt ttttattttc tcctgggtta actttctttt agtagatatc
     2881 tctaaacttt ttttataatt atctattgca gaattataat tttcgctttt atattctaag
     2941 tttcccattg ccatataata gtacatatct ttatctaata tataaactgt tctatctgat
     3001 tttataagat aatcagcttt ttctaaaaaa gtctttgcct tttgtatatt atttttgccc
     3061 acatacgcat ttgactgcat tatagaggcc attatatcaa aatcattgta attatccgta
     3121 tttacaaact ttttataatc ttttatcctg ctacttattt caatagcttt atcatattcg
     3181 ccctcttcta catatatttc tgctagattt ataatagcat ataatctaac cattccattt
     3241 ttttcacctt catcaatatc tattttaaaa gaatccttta ttaatttctc tcctgtttca
     3301 tagccaccta tctttaaaaa tatgctcgcc aaatcaacat ctagtcttgc tttttcataa
     3361 ttattcccca atttctcagc caactttata gactttacag ttaaatctac tgccttagaa
     3421 taattattat ccattatata cactgatgct aatctttttt ctatattaaa tctacattca
     3481 tcagataatt tcacatctga ttctctcatc ttttctaaag aatcaatgac tatcttcgtt
     3541 ccatctggaa catatgaaat agtctttaac atatcccaaa cttcattata tactttattg
     3601 tatttttttt ctggaataga ttcaaaagaa gtcttaacca ctttttttgc ttcatcatac
     3661 tccttatctg ccacaaggct ttcagacata tattttccct tatttataat ttctctttct
     3721 ttcaatccat tttggtcata ctttataaac aaaataatac taataagtac tatacatata
     3781 aaaattatct ttttgggtaa ccttttcttt ggtagtctaa aattcaaaat tttagtcctc
     3841 cttttgtata ttgtagaatg aaaacttgca tatataaata acctcttaat aattaataat
     3901 acaatatatc aaaatgattt acaacatatg ctagacttaa atcttaataa aaaagtctcc
     3961 taaaataatt taggagactt taaatttaag ctaatataaa tttatacagt taaaacaagt
     4021 gcttgttttt tagttttata tttaatattt atttatacat atttttttag tactattgta
     4081 gcattatgcc caccaaatcc taaagaattt gatagtgcat atctaatatt tttttcttta
     4141 ccttgatttg gaacataatc taaatctaaa tcttcaccaa cttcttgtat gtttatagtt
     4201 ggaggcacaa aactatcttc tatactcata gcacaaacaa cagcctctat agcacctgat
     4261 gctcctaata agtgaccagt catagatttt gttgatgaca cacataagtc atatgccttt
     4321 tctccaaata cagtttttat agccatagtt tcatataaat cattgtaata agttgaagtt
     4381 ccatgagcat ttatataatc tacttcttct aatggaacat ttccatcttt caaagccata
     4441 ttcatagacc tagctgctcc ctctccattt tcagctggag ttgtcatatg gtatgcatca
     4501 gcagttaaac catatccaac tacttctgca tatattttag cgcctcttgc taaagcatgt
     4561 tctaagtctt ctaagataac aatacctgct ccttctccca ttacaaaacc atctctgtct
     4621 ttatcaaatg gtcttgatgc agttttaggg tcatcatttc tagttgacat agccttcatg
     4681 ttacagaatc cagcaaaagc aagacctgtt acagcagctt cagaaccccc tgctaccata
     4741 atgtctgcat cacctctttg aattacctta aaagcatcac ctattgaatg agttcctgat
     4801 gcacaagctg tacatacatt agtgttaggc ccttttgctc ctaaaaatat tgaaacttga
     4861 ccagctgcca tatttcctat catcataggt acaaaaagtg aacttactct tttatttcct
     4921 ttttccaaaa gaattttatg ttgagtttct atggcttcaa ctccacctat tccagaacct
     4981 attataacac caaaacgttc tctatcaact ttatctaaat caagatttgc atcttcaact
     5041 gctaatttag cagatactat tgcaaattga gcaaatttat ctaatctctt caattctttt
     5101 ttacttatat attcttctgg atgaaaattc ttaacttctc ctgctatctg agtttgatat
     5161 gtgctagcat caaaattagt tattttatct ataccagata ctccagattt tatattactc
     5221 cagaactctt cttttcctgt tcccaacggt gtaacacatc caagtcctgt tattactact
     5281 cttttattca tcggatacct ccactttttt aaagctcgac tatttattag cttctatata
     5341 cttaactata tctcctatag atttgaagtt ttctgcttct gtatctggta tttctatacc
     5401 aaattcatct tcaagtgcca ttattatttc tactgcatct aatgaatctg cttctaaatc
     5461 cttcattaat gatgtctcca tagttatttc tttatcttct actcctaatt gctctattat
     5521 tatttctaca actttattaa acattgtgta cctcctaaaa ttttttattt taatagattt
     5581 ctctactttt taaattatta ttgcattacc attccaccat ctacatttat tacttgtcct
     5641 gttatataac ttgacatgtc actaactaaa aatgctacgg cattggctac atcttctggt
     5701 ttaccataag atttctttgg tatgtttgct agtgttgatt cttttacatc atcacttagt
     5761 actttagtca tatctgtttc tataaatcca ggtgctactg catttatatt tatgtttctt
     5821 gatgctaact ctcttgcaga agcttttgta aatccaatta cacctgcttt tgatgcacaa
     5881 taattagttt gccctgcatt acccataatt ccaactactg aagtcatatt tattattctt
     5941 ccatacttct ttttcatcat aggtttagta actgcttttg tacaattaaa gacacctttt
     6001 aagtttatat ctataactct atcaaaatct tcttctttca ttctcataag cagaccatct
     6061 ttagttatgc ctgcattatt aatcaatata tcaatttttc caaattcgct tacaacagaa
     6121 tctatcattt gattcacttc atcaaattta gacacatcac attttataga ggtacacttt
     6181 acccccatac tttctatttc atttttagtt tttagtgctt catcttcttt agaagtataa
     6241 ttgattacta catcagctcc aaaagatgct agtttttttg ctatttcttt tcctataccc
     6301 ctagagccac cagtaacaac tgctacttgt cctgtaagat ttatcattat atctccccat
     6361 ttctataatt ttgtacaaat tcattaagag aatctatacc atctatatta taaatattga
     6421 catttgcttt tttattggaa gctatcttct taataaatgc ttttaaagtt tttccaggtc
     6481 ccatttctat aaaagtatca tatccatcat ttaaaagtct ttctatagaa tcttcccaaa
     6541 gaacagatga acttacttgt tttataagca ggtctttacc ttcgttttta tagtaatctg
     6601 catttacatt tgccaccaca tcaactttag gtttagttat gttaactttt tctaattcac
     6661 tggccaattt tacaccagct tcttttaaca ttgatgagtg aaaaggtcca cttacattta
     6721 atttaactgc cctttttgct cctgcttctt tgcattttac cattgcttct tctatctttt
     6781 cattttcacc agatataact atttgtccag gagaattgta atttgctact tctacaatac
     6841 ctccttgtat atctttaacc acattttcaa gtacatttct atctaaacct aaaattgctg
     6901 ccatagctcc aacacctgct ggaactgttt cctgcataaa cttacctctt tttttaacta
     6961 aacatacagc atctttaaag tcaattgcat ctgctgcaac caacgcacta tactctccta
     7021 aggataatcc taagcatgca ttatatttta aatctatttg agcttgtacc gcttttaata
     7081 ctgctacact atgtgtaacc atagcaggtt gagtgttttc agttttagaa agttcttctt
     7141 catttccatt aaacataagg ttttttaaat ccatatttaa gacatcacta gcctcatcta
     7201 ttacactttt agcaacagta ttattttcat ataaatcctt ggccatacct acatattgag
     7261 ctccttgacc agggaatact agtgcaactt ttcccatctt gctcctcctg atttacagtt
     7321 ctattgctgg cataatctct cttgcttgat ttaccatagc ctctataatt tccttacaag
     7381 gtgttatttc ttttaccata gcagcacttt gacctgccat aaatgaacct ttttctatgt
     7441 ctccatctac tgttgcaaat cttaaagctc ctgtaccttt tttatccagt tcttcaggag
     7501 tagctccatt ttgttccatc tttaaaaatt cttttgatag tttatttttt aatgttctta
     7561 ctggatgacc agtacttctt cctgttacaa ttgcatctct atcttttgct tttagtacta
     7621 agtttttata gtttgaatgg acagaacact cttcactgca aataaatcta gttcctactt
     7681 gaactgcact ggcacctaat gcaaatgatg ctgcaattcc tctaccatct acaattcctc
     7741 cagcagctat tacaggtatg tttacagcat cagcaacttg tggaactaag accatagtag
     7801 taagttctcc tatatgtcct ccaccttcag taccttctgc tattactgct gtagctccta
     7861 gcttttccat tctttgtgcc aaagctattg taggtactac aggaataacc tttgttccag
     7921 cttcctttaa tctatccata tactttgcag gatttccagc accagtagta ataacttgaa
     7981 ctttttcttc tataatcaaa tcaattatat catcaacaaa tggcgacata agcattacat
     8041 ttactccaaa aggtttatct gttaattttt tacactcaac aatttctttc tttatagctt
     8101 cttttggtgc gtttcctgct gctattatgc caagtcctcc tgcattagat acagcacttg
     8161 ctaatgatgc agtagctacc catgccattc ccccttggat aactgggtat tttatattta
     8221 atattttgca aattttattc atacaattct ccttcacttg aaatcataaa agtaaacttt
     8281 ttattattaa tcaactttat tttgtgtata agtttaattt tgttaattaa atcattttag
     8341 atattttata ctataaagtc cacttgacaa ctgaagcccc ccaagttaga cctccaccaa
     8401 agccaactaa aactacatta tcaccctttt taattttacc ttctctataa gcttcatcta
     8461 atgctactgg tatagatgca gcagacatat ttccatattt gtctatattt acataaactt
     8521 ttttcatatc aagctctaat ctcttagaag aagcttgtat gattcttata ttagcttgat
     8581 gtggcactaa gtagtctata tcagttgtat ttagatttgc tttttcaaga gctcttaaag
     8641 aagtttctgc catctttcta actgcaaatt taaacacatc actacctgcc atttctattg
     8701 tatgtagatt agcttctaca gtctctttac ttgccttaag tcttgaacct ccagcaggta
     8761 cttttaaaca atcttttcct ttaccatcag agcccatatg tgtagacagt attccaccct
     8821 catttacagg gccaataaca gctgctccag ctccatctcc aaacaatact gctgttgttc
     8881 tatcatcata atttaaaaac ttagaacaag tttctgctcc tattacaagc acatttttat
     8941 agcaacctgt ttccacaaat tgctttgcaa tagttactcc atatacaaat ccagaacaag
     9001 ctgctgatat atcaaatgca gtagcattca ctgctcctat tgcatcttgt actatacatg
     9061 cagttgaagg caaagacata tcaggagtaa ttgtagctaa tataatcagc tctatatcct
     9121 ctacgtttaa ctttgcacat tctatagctt ttaaggcagc tttagatgct aagtcagaag
     9181 ttgcttcaga ctctttggca aatcttcttt ccttaatacc agttctagta cttatccact
     9241 catctgatgt atccattatt ttttcgaagt gaaaattatc atatgattgc tcaggtaaat
     9301 aacttcctac tcctaatatt ccagcctttg tattcattat atattaaacc tccaaaacga
     9361 gtttattcat cttcgttatt tttattttct tcattgtatt tgcttataaa ttggttaata
     9421 tcttcaacga cttttccctt tgaaaatttt attccttgat ttatagcatt ttttatagct
     9481 ttagcatcag aactaccatg agcttttata actcctccat ttagacctaa aagtggagct
     9541 cccccatact cagaataatc tataaaactt tttaattttc ttaaatcatc ctttataagt
     9601 aatgctccta ttttactttt tgtactggcc ataaaagttt cctttataag actcataact
     9661 gaaagtgcta caccttcagc agatttaagt aatatattac ctgtaaatcc atcacatatg
     9721 attatatctg tgtatgcatt tataacttct ctagcttcta cattccctat aaaatttaaa
     9781 tctaattttt taatttcttc atatgacctt ttgactaaat cattgccttt gccttcttct
     9841 agtccaacat ttgctaatgc taccttagga ttttcaagac ctaaaacttt tcttgaataa
     9901 atattactca tagctgcaaa ttcaactaga ttcttaggtt tacaatctgc atttgcgcca
     9961 cagtctgcta taagtgtcat tcctcgcttt acatttggta ttgctgaaca taagcaaggt
    10021 ctatcgattc cttttattct accaacaaca aataatccac cagcaagtaa ggctcctgta
    10081 tttccagctg atattatagc atctgccttt ccttctttta caagatttaa tgctactacc
    10141 atagatgaat cttttttact tcttatagcc tttactggtt tatcttcgtt ttctataatc
    10201 tcagtagtat gaactatctc taatttattt ctatcaaatt cataatttga aaattccttt
    10261 tcaagtaagt ctttatctcc tgtgattatt aaatcaacct gatattcttt tatagcattt
    10321 actgcgcctt ctacattaga tttaggtgca ttatctccac ccattccatc aattactatt
    10381 ttcataattt ccctcctaat ctaattcttc aaagataaac tttcctctaa atatttgctc
    10441 ttgggcttta ttgttgataa caacttttac ataatgcttt ttatctatat ttctatacac
    10501 ttctgctttt gctataagcc tatcttgttg ttctatcaac ttaaaacttt ttacatttgc
    10561 aactctcata ataaccttag gtgcatctat tatagccatt gccaatgaat ctgctagaga
    10621 aaaaatatac gtatctttta aagtattagt cttagagtaa atcatgtcct gtgtaacttc
    10681 taacatagat atacctaatc gccctacaga taaatcaatg atttctcctg taatttcctt
    10741 tactcctaaa gtctttactt ttgaactctg agtttctgct attgatttga ctctttctct
    10801 tagctctggt atgtttagac tcattctatc taatcttatg gtctgtatac ttacatcaaa
    10861 cagacttgat aattcttcat cagtataaaa aggatctgtc tttagcatat ctataagctc
    10921 tttttgtctt tgggctttac ttttcttttt catgttccct ccaaatttta tagctgtaat
    10981 tagtaccagc tactaatagt agtatatagt aacacacaaa aattttcaat acttatatta
    11041 ttatttttct attttttaat atgcataaaa tccacttatt ttctctagta aatcatctta
    11101 tttttttata tgtacaaaaa aaacatgtag agtatactct acatgctttt tagaagctat
    11161 tacttagaca caacttcttt acctttataa tatccacagt ctggacacac tctatgtggt
    11221 aattttggct catgacattg tgggcaactt acaaatcctg ttgcttccat ctttgagtta
    11281 gctgctcttc tcatttttgt gtttgattta gacgttttac gctttggtac tgccatttac
    11341 gccacctcct tagtcaaaat ttaattaaat aaatctttta atttagcaaa acgaggatct
    11401 atgtattcct catcgtttgt aatttcactg caagaacact cttcttcatt taagtttgcc
    11461 ccgcatactt gacaaagacc tttacagttt tcactacaaa gaactttatg aggtacatta
    11521 aaatctaatg tctgttctat tatatcaata aggtcgattt cttcaccatc gaatataaaa
    11581 ggatcaaatt cctcaaactc atcttcatca taatcttctt cttttactaa aaaaccttgt
    11641 atagaatact ctagtggtat ttctacttct ttaagacatc ttgaacaatt gtcaacaatt
    11701 gtaaaattga catctatatc tagatacaaa cctttattag tctttgacac tttccctttt
    11761 aaatttacag gtgatactaa tttgtaaatc tcatcacaat aactaatagt atcaattttt
    11821 tcacaaaaat tcaagtctat tttgtcagtt tcttttctat ttattttttc tatgctaact
    11881 ttcattaagt tcacctcaac atatcaccaa atttattata caaatctagc atttgtttgt
    11941 caagtatttt tacttgatag gcataggttt aatttaatta ttttaccaat cttaatgtat
    12001 ctctagctat cattaattct tcattagttg gtattaaaag catttttact ttagaatcag
    12061 cagtagatat tactgtttct tttcctctaa catcattagc ttctttatct aatttcattc
    12121 ctaaaaactc catgttagaa gcaatatctg ctcttatagc ctttccattt tcaccaacgc
    12181 ctgcagtaaa tactacaaca tccaatccat tcatttcagc agcataagcg cctatatatt
    12241 tttgtacttt tttaacatac gcttctaatg tagcttgagc tctttcgtct ccatttttag
    12301 ctgcatcttc tatatctctg aagtcactag aaattccagt cattccatat actccagatt
    12361 ctttattcat taatttatcc aatccatcag catctaaacc ttcttttctc attaagaatg
    12421 gtaatatagc tgggtctata tctccacatc tagttcccat tattaatcct tctaatggag
    12481 tgaatcccat agatgtatct acacattttc caccatcaac agcagctata gaagcaccat
    12541 ttcctaagtg acaagttact atttttaaat cttttatatc ttttcctagt atctctgctg
    12601 ctctctgaga tacataatta tgagaagttc cgtggaatcc atatcttctt accccatatt
    12661 tagtatatag ctcatgaggt aaaccatata agtatgaaga tttaggcatt gtttgatgga
    12721 aagcagtatc aaatacacct accattggta catctggaag tatagcttta caagcatcta
    12781 ttcccattat gtttgctgga ttatgaagtg gagctaaatc tatacattct tttaaagctt
    12841 cttctacttc tggagttatt actactgaag ttgcaaattt ttctccagca tgtacaactc
    12901 tatgtcctac tgcatctatt tctttcattt ctttaactgc accaaattct ggatgagcaa
    12961 cagcttctaa aactaatgct attgcatctt tgtggtcctt cattggttgt tcaacaacgt
    13021 atttatcatc tctacctgct ttttcatgtt ttaatataga accttctatt cctattcttt
    13081 caaccaatcc tatacataaa acttcttcat tattcatatc tattaattga tattttaatg
    13141 aagaactccc acagtttaat actaatattt tcattattat ttcctcctta gattttttta
    13201 taaataatat gtaataaatt
//
LOCUS       DAMJYC010000106        13189 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJYC010000106 DAMJYC010000000
VERSION     DAMJYC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.110"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            381..635
                     /locus_tag="PC472_003249"
     CDS             381..635
                     /locus_tag="PC472_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728553.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            671..994
                     /locus_tag="PC472_003250"
     CDS             671..994
                     /locus_tag="PC472_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728554.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLSQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1268..1420
                     /locus_tag="PC472_003251"
     CDS             1268..1420
                     /locus_tag="PC472_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728555.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1614..2537
                     /locus_tag="PC472_003252"
     CDS             1614..2537
                     /locus_tag="PC472_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2728556.1"
                     /translation="MVGAFVLPNYAITLSEMSNLWGNFTMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMALVSVLFFTYSIYLCATWKDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VEKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   2804..3616
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2804..2832
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            3771..3959
                     /locus_tag="PC472_003253"
     CDS             3771..3959
                     /locus_tag="PC472_003253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728557.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4079..4892
                     /locus_tag="PC472_003254"
                     /pseudo
     CDS             4079..4892
                     /locus_tag="PC472_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            4944..5105
                     /locus_tag="PC472_003255"
     CDS             4944..5105
                     /locus_tag="PC472_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728558.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5433..5897)
                     /locus_tag="PC472_003256"
     CDS             complement(5433..5897)
                     /locus_tag="PC472_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728559.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6033..6350)
                     /locus_tag="PC472_003257"
     CDS             complement(6033..6350)
                     /locus_tag="PC472_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728560.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6633..6929)
                     /locus_tag="PC472_003258"
     CDS             complement(6633..6929)
                     /locus_tag="PC472_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728561.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(6948..7361)
                     /locus_tag="PC472_003259"
     CDS             complement(6948..7361)
                     /locus_tag="PC472_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728562.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7345..7578)
                     /locus_tag="PC472_003260"
     CDS             complement(7345..7578)
                     /locus_tag="PC472_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728563.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7797..8171
                     /locus_tag="PC472_003261"
     CDS             7797..8171
                     /locus_tag="PC472_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2728564.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            8783..9475
                     /locus_tag="PC472_003262"
     CDS             8783..9475
                     /locus_tag="PC472_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728565.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            9671..11731
                     /locus_tag="PC472_003263"
     CDS             9671..11731
                     /locus_tag="PC472_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2728566.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(11937..12260)
                     /locus_tag="PC472_003264"
     CDS             complement(11937..12260)
                     /locus_tag="PC472_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2728567.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12253..12954)
                     /locus_tag="PC472_003265"
     CDS             complement(12253..12954)
                     /locus_tag="PC472_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2728568.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
BASE COUNT         5366 a         1343 c         2039 g         4441 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaaa tctgtgactt tagtcatggg aggttcaggc
       61 tagtattact cattattgta ttcaaaagct aaagtggaaa cctagtgaat atatgaattt
      121 agaagttaat gagagagcat tagcagccga ctcaatactt attaaaattg aagatgaaga
      181 agaagcaatg aaagaagatg aaagagagaa aaagagaggt agaagaaggt agaatttaaa
      241 gttaaaatag tcacattgct aatatatgaa aatgttacaa ttttggaaat agatggatat
      301 atatgttgta tagtataatg tagataatat atcaccaagt aaaaaacaac aaataaatct
      361 aaattatgag gggtgtattt atggaaaata aagatattaa acttacaata tgtatatttg
      421 cttttgtaat tggaataata gtgttatttt gctgtatagg tctaggtcaa tttaatatgg
      481 attgtattat aaaacaaaat ggtgggtcaa tagctaccga acaatacaac atatatttag
      541 aacaatctat ttcacaatat agaaattttg gttctatact ggttttacta ggaggaatag
      601 gaatactgtt tgataaaagt gtcagaaaat actaaaaaag tatattttta tttaggatgt
      661 gatgaatgat atggcaagag atgtatttag tgtaaagatg acaccactag aagcacagaa
      721 aatggtagaa aaaaatttgg aagcagattt agtttctgtt gatatttata acctagaaaa
      781 taataagtct ataatagtta caatctttga aaaatattat gcaagaagca atagtgatgc
      841 aggtttggta gtgatttgtg aaaatactac aggaaaaaca cttgttaaaa ttacctcaac
      901 tggtagtgca attggactat ctcagattga ttggggaaca ggtaataatt tgattaaaag
      961 agtaaaaaaa atacttattg attatattat ttaattattt tatgtagata ttcttaggtt
     1021 ggtatggaat ttgtactaac ctattaagta atcaataaaa ctaaataaac atagaaagca
     1081 cttacaaata tgtaggtgct ttttattgaa aaaatagaaa ataaaataaa aagtaagata
     1141 taggaaaaat atgtaagaat tatatattat aataattgta gcaagaagat gtaatctaca
     1201 atttatagag tggagttcat gtattaaaaa attatcctcc caacgataag aagggaggtg
     1261 aatatacatg gataattttt tatttaatgt gttagctagt gtaacagcta gttacatagt
     1321 ttatttgatt agcaagttaa tcaaaaaagt aaaaagccac tctaccgcaa agagtgactt
     1381 aaagattgat ataaatttta aatatcatcg taaaaaataa ctttcagtta tagaacttca
     1441 ctctaccgca aatagattgt agttcttctt gcttttatta taccacaaat tagaaaaaat
     1501 attgcatata atatttttta tagtcaataa aaatatgaaa ttttttatac acaatgatga
     1561 tgaatctaac tagatgaata aatttaattg taaataagaa agtacttact taaatggtag
     1621 gtgcttttgt tttgccaaat tatgctataa cattgagtga gatgagtaat ttgtggggga
     1681 attttacaat gtggatatta gtaccaataa taacaatagt attacttata atagctgttt
     1741 cttctatgca atatatttta gttatgatag cttttttgtt gattatatat tcttttatag
     1801 aaaagaaaat agttatggct cttgtgtcag ttcttttttt cacttattca atttatcttt
     1861 gtgcaacttg gaaagataaa tccttgatag cagataataa agttgagact gttaaggcac
     1921 aaagagaaac tgtagaaaga gaaaaagaaa tggaaagaag aagaatacaa gaagaagtag
     1981 acaaagaaag atatattgaa aaacatggaa tggaaatatc agaaaatgat ttaaaagtta
     2041 agctagaggc tttagtacct caagaatata aggggaagaa atatgaatta aaagtagaaa
     2101 aatttaaaag atatagtatg tattttgatt taactgtaca aaatgaaaaa tttagtaatt
     2161 ctgaagaatg taaaaaattt gtaaaagaaa ttgctaatga tcttaaaaaa attaaaataa
     2221 gcaaggcgta ctttaagttt cattcaaaag atgacggcgg tatatataat tctgtttata
     2281 ttgattattt tagaaacata caaaataatg tagataatgt tgaaaattta gaattcaatg
     2341 agtttgaact taaaaccgaa gaagaagaga agcgagaaca agagaaaata gaacaagaaa
     2401 aaaacagtta taataactac attcaaaaca gagtggttga cccactagat agaataaaga
     2461 aactaaaaga gttgctagat tcaggagcaa ttacacagga agaatataat aaaaagaaaa
     2521 aagaattatt agaatagata atatagtaag cacttacaag tatgtaggtg cttttaaatt
     2581 tacaaagtat tccattttaa ttttatagtt tagattttat gatataataa aaatatagaa
     2641 gttttgcagt gtgcgatatt tgttacaaag tagggcttaa tacttgaaat ctaagatgtt
     2701 gagggtgcgt gataagtgtt atcaattgca ctattgcccg ctcactgcaa ttttaagagt
     2761 attgtatata tgtaggtatt ggaaatgcta agtttatttt ggggttttag attaactata
     2821 tggaatgtaa atgaaattag ttgaactatt gcttgatatt ccatatacgt tttagattaa
     2881 ctatatggaa tgtaaatcaa taaacccaaa aacatttaaa gaatacatgt ttaagtttta
     2941 gattaactat atggaatgta aattgcttac gaatggttgg aattacatct attgctaacg
     3001 ttttagatta actatatgga atgtaaatca atatgatttt gcattagatt ttagtgaagg
     3061 attagcgttt tagattaact atatggaatg taaattttct ttaaaacaga catcaatttc
     3121 gttatatctg gttttagatt aactatatgg aatgtaaatt tagaatcaaa agcacttaat
     3181 cctagttctt tcatagtttt agattaacta tatggaatgt aaatagcttt ttagcttcgt
     3241 ctactacttt ataagcaaag cgttttagat taactatatg gaatgtaaat aaaagttgca
     3301 cgcctatttg atttttcaat acattatgtt ttagattaac tatatggaat gtaaatgaca
     3361 aagcgactat tactatctca ggtgataaaa atgttttaga ttaactatat ggaatgtaaa
     3421 tgatgaacaa taaaacaatc atctaaagac gaagaaagtt ttagattaac tatatggaat
     3481 gtaaatgttc ctgttgtgtt ttttactaat cccattttat atgttttaga ttaactatat
     3541 ggaatgtaaa ttgctgtgat ttcactaata aaacaattaa taattgtgtt ttagattaac
     3601 tatgtggatt caaaattaaa taaacaaaga aagcacttac aaatatgtag gtgcttttat
     3661 tctgctcaaa attggtcggt tgggtaaaat cattagaaaa aattagtaaa aacctctttt
     3721 ctgtaactcg ttacaatatt attattaatg taacgagtta cagaaaagag gtgaataaaa
     3781 tagcaactaa aagtagggca gagtatatga aaaattgtcg aaaagataaa agaggtttta
     3841 gtgtactttt agacaaagaa aagttagata aatttgatga agtattagag gaaaagaatc
     3901 taaccaagaa agaatggcta gaagaaaaaa tcgacgagga actggaacaa aaggaataaa
     3961 aaataagggt cactcccacc gaccaaagtt tgagtaaccc ctatgatgta tactatcgta
     4021 tatcaattat aatatatgtc attccttaaa aaaatcaatt attaaggagt gtaatattat
     4081 gaaaaatgaa ttaatgatgt ttgaaggaaa agagattgaa gtatttgaat ttgaaggaag
     4141 aattttattc aactcaaaag atgtagcaaa ttgtttagac ataaaaaatg ttaatgagaa
     4201 tataatactt atgaatgaaa agcaagttgt aaagttaaga aattcggata tcagtaatac
     4261 tgacatccga aaacttaata atgctggaga aaattttctt actgaaagtg gagtgtataa
     4321 attaatcttt aaatcaagaa aagaagaagc tgagagattc caagactgga taagcgatga
     4381 agttttacca agcataagac aaactggtgc atacataact aataatgtaa tcccgaaaag
     4441 ttgagagaaa aagcaagtga gattgaaaaa ttacagttgc ttataacagt acatctatgt
     4501 taaaagaatt gctagatgat gcaggctttg acaataaatc aaaactatta acagctaaaa
     4561 cattatataa gaaagcagaa attgatttac caatagagat aaacgaagaa gaacattatt
     4621 ttgatacaaa acaaatagca tctaaactga aaatatattc taagagtaat aaaccagctc
     4681 agatggctgt ttgtgagatt attaaaaaga ttgatttaga agatggtgaa gttaaaggtg
     4741 tttgggaaat taatggttct tggactggta ctgtaaataa atatacaaag agtgtaatag
     4801 ataaggtgag aacttggata gaggaaaata atagacctac caagattgca ggtgagaaga
     4861 agaattatta tgtattttac aaaattgagt aaatttatca gttgtattaa ataatttagt
     4921 ttagttttgg ggggattaat acaatgtgtg agaatttact tgataatatg tatattgaaa
     4981 agagaaaaga agaatataga attaaacttt tgaaaataag agaaacagat atagatatat
     5041 ataataagtt agaaagtata gtatataaac tttctgagaa aaaattagag aaaaataata
     5101 aatagacata taaagcactt ggatattcta ctgtttcaag tgcttgtttg gtataaaatg
     5161 gtataatgga gatagagaat tatattaacc aaaaaatgga gaaaatagag aagatgattt
     5221 agttgacgct ttatcagtag gagtacctgc aggtaagaca gaatctctag tacgattagt
     5281 tggagatgga ataagtgata ctcaaaaaag tttaataaat aaaagagaat ttagaaatat
     5341 aactcaaatt ggtggaaatg gaaatgagaa agcatttagt gaagtagaaa acttagttaa
     5401 atagattaga aggtagcaac tagagttggt tgttaccttc tttttttata ctttctctct
     5461 ctaagtattt tatttttcat atcttctaat acatctaatg cagtagtata aaattgtata
     5521 ttatttctat aaggtatagc tattggaaga atgcccttgt taactaaaaa aataagaagt
     5581 acaataaata aaagtgtcat aatcattcta ataaaataat tagtgatatc tgataaatca
     5641 aatgttaagt taattgttgt agaaaataca acagccataa tagatatata caaagcaaaa
     5701 atggggttac tatttttttg taactgtcct ttaagcctag attgctctat ttctatatct
     5761 tgaatacttt ttatggaatc catttcacta taatacttat aaacttttaa ataatatttt
     5821 ttatacttgt ttaaagcttc ttcattatct ttaaggtctt tgtcacttaa ctctgattta
     5881 acttccccaa aatccataaa atccctccta caatgttata taatatactt taatttatag
     5941 catatttaag aacttatatc agcaatttgt cgaacgattt tagctaaaaa tagagtataa
     6001 tgataggctt attatttata agatattatt ttttatatag ttatatattt ctttctaatt
     6061 agataatata atgctgttgt tataatggct actaatatta ttgctattat tgtaatatct
     6121 aatcttcttg taaataagcc tattgctata tactcaagac ctgttacata gcgataaagt
     6181 acttctagtt taacagtttt tttcttatct atatttccat atattgattc taatttatta
     6241 aaatattttt tactacaaaa agcagtttta ggtttaatta aatatattat tgtagttcct
     6301 attataacta ttattccaat aagtataaga ttgatatttc caaaatccat agcagatacc
     6361 tcctattaaa tttgcttttc ttaatcaaag tatatcagta acacaagtac aaagagtatc
     6421 taaaatatta caaatattaa caggatagat aaaactatag taaaaattgt aataataaag
     6481 cgaacgaaaa ttatcatact aaaacataag acatgttata attgtattag atagaagatt
     6541 aacatacacc aaagcactcc ttataataaa gagtgcttat ttttttgaaa ttcatcaata
     6601 tataaactat caagaacctt attcaacaca ccttaaaatc gatttaaata gttctttttg
     6661 ataacataat ttatatgata taataaaaaa atagaagtgt accaccaata cacttctata
     6721 gctataacta agcattctct tgctggggga gtgctttttt catttctcca aacaacatta
     6781 tttacttcat cagtaattat caaatcaatc tcattatctt tcaaaatctc tgttgcagta
     6841 caaattagat tatctaagtc aaaacttcga gttatctcta gttgtttgtt atttacttct
     6901 ctttttagta tataagtttt aaaatacatt tatatcaccc cttgtattta ctcttttttc
     6961 tctaaaaata tattgataag taaatatgca acaaataaaa aaatctcaat gacacatagt
     7021 gttatagcta taactagaag tgattgattt ttccatgcac tataagctat gatgaaaaga
     7081 aatgtaaaag taaataatgt ataattcata attttaacta agatatgact tgttttcatt
     7141 gttaagtaca agtctctttc atctgtacta tcgtgtaatt cttcaacaat gccttttctt
     7201 gaaaaagcat tacaaaaatt aaagattgct attacaatta aaagaaatga tgaaattagg
     7261 aatcttttat cttttaaaat gcaataaata actaaactag cacaacataa cacacaaact
     7321 ataataccca ctataaaact tcgtttactc tttactttca tgttttttat cctccatttc
     7381 tttattttct tttaaacaac atagttcttc aatcgttgta ccaaacactt gtgccatacg
     7441 ataggcaagc ataagagaag ggctatattg ctctttttca atagaaataa ttgttctgtt
     7501 tgatacatgg actaaatcag cgagttgttg ctgtgttaga cccatatttg ttcgaaattc
     7561 tcgtactttt gttttcattc cataacctct ttctatatga agctaacttc atgtgaagtt
     7621 aacttcataa tatcctaaaa tgagtaaaat gtcaatctca ttagataagt attataaaac
     7681 ctaaaagaat aatatggtaa aaattggatg gaattattta ccttaattct cttgtttgta
     7741 aaactttatt gatatagaat ttaacatata cataaatcgg caaaggggcg cgaattatga
     7801 aaattagtaa attaccagaa gcggaattaa aagttatgag atatatttgt gaagctaaca
     7861 aggtattaac ttctagggaa ttagttgctg caatggaaga aaaatatgag tggaatgaat
     7921 caactacatt tacagttttg aaaagattag agcgaagaga gtttttaagt acagagaaaa
     7981 taggcaaact tacacattat aatatactag taaaagagaa aaaatatcta aggtttgaaa
     8041 caaaggaatt tttgaagaac atacataaaa attcaatttc aagtttaatg tcagcgttac
     8101 atggtgaaga cgacgaagtg gatgaagata aattaagtga actcgaagaa tgttttaaga
     8161 atttgaaata agaaaatgac aaattgatac ataataaata aaatgaaggt atgaaattat
     8221 aactttttct accttcattt tgtttattat tctgaacatg ttaagaaaat gaaataagtt
     8281 tgtattttag ttatataaat tttaatagaa tatgtacagt atatagtaga tagaatgtat
     8341 aaaaatagaa tttaaataag tagctattat tgcagtgaaa tgaatgtttc taagtatatt
     8401 tgggtggaag aatggtggaa gaaaattagt gaaatgtaat tcttccacca ttcttccacc
     8461 tagaaaatag aaactgttta ataagaatta atatacatta attcagctga atgcaatcaa
     8521 aatacttaaa tgaaagtaat ctgaaaacaa cataattcaa atcaatttat tcaaataaac
     8581 ctcaatttat cttaaatctt aagaaatatt taagaattag gataatattt tcttaagatt
     8641 tatattttat tatatagtta taagggaaag ttacaaaaat gtaagccaaa gtaatataac
     8701 aatttagtca ttgttgagaa agggagtgaa agatattgta taatatagat aagtattatt
     8761 atacaataga ggaagtgaaa atatgtacaa tatattagta gtagacgatg ataaagaaat
     8821 tgttgattcg atagaaatat atcttaaatc agaaggattt aaaatatata aagcatatga
     8881 tggaatgaaa gctttggaaa taataacaga gaatgacatt catctaatac taatggatat
     8941 aatgatgcct aaattagatg gaatcaaagc aactgtaaaa atacgagaag aaagaaatat
     9001 tccaataatt ttaatttcag caaaaagtga agatacagat aaaataatag gattaaatat
     9061 tggagctgat gattatataa caaagccatt taatctatta gagcttatag ctagggttaa
     9121 atcaaactta agacgatatg taactttagg taattatgaa gatacatgta aagaaattct
     9181 aaaaagtgga gctttggagt taaatacact aacaaaagaa gttaaaattg atggtgaaaa
     9241 cataaaaatg actccaattg agtataaaat aattgaactt cttttagaaa ataaaggacg
     9301 agttttctca atagatgaga tatacgaaaa agtatggaat gaggaaagtt ttaattcaga
     9361 aaatacagta gcagtacata tcagacgaat aagagaaaag attgaaataa atccaaaaga
     9421 accaagattt ttaaaggtgg tgtggggagt tggttataag attgaaaaat tttagtggaa
     9481 aaattataaa agaaaagagt ttaatattta attgcatttt gattttagtc atttttacat
     9541 ttacacaaat gttaggaatt ttaatatgtt ttaatttttt ctctgagagt ttaatttact
     9601 ctatggggat tatgcttttc ttaaacttta ttttatataa aatactgaca atgtagatga
     9661 ggtgggcaac ttggagaaat atagagtatt aaagttagtt tcattaatca taatatctat
     9721 ctcatttcca ttaactatat tgttggtgac aataggactt gctgagccta aatacaatat
     9781 acaaaaaact tctacaaaga gtgggatttt tgatatatct gatgattttt atgatagtaa
     9841 tatatttgac gaaggaacaa tggcaagtct attacccaag tttaagctat ctgtaggtgg
     9901 taaattaact agtaaagaaa aaggtaaatt ccaacaagaa ggtaaaagta tagatttaag
     9961 tagtagtgat tctgagtatg ataaaatcat gattaatgaa caagcttctg aagaatattt
    10021 aagttatcta aaaaatataa aagtgtatgt tattaataca tacacaggag tattttatac
    10081 aaatacgtca tatgctagtt tatatgattt taaacaaaaa actaagagct attgtaatgt
    10141 agagataata aataagatag gaaagagctc atatattaaa acaataaatg gagaaaaatt
    10201 tgaactgaat aatctgagtc tggaactaaa tgaagtagat gaaggttttg aggcatatgt
    10261 atcttttcca gaagagccaa ctatagaaga tggaatagta tatactaatt ttcaaatatt
    10321 taaacaagct acagaaaaag taagattatt ttttgtagct agtttaagtt tagcaattat
    10381 atgtttatta tctataattt tatatatcaa aataaaaggt gaagttttaa aaagaaataa
    10441 tgtattttta ctaatataca gtaagatacc tatggaattt aatgtaatat tagtatctat
    10501 ttcaattgca attttaacta tatatgctct tgctgtatct tatcatattg atgtaattgc
    10561 attagtttct ttatctttac tgatagtatc aaataatata ttttttataa atgtgcaatt
    10621 gagttattta gaaaataaaa tgtgtatatt taagacaagt attatagtaa gagtgatttt
    10681 atggttaata aaaactgtaa gggaggtcag taacgctata aagaaagtat ctttagcaag
    10741 gaaagttatt gttatagtga taatatcttt agctttaatc atggtaatta gcactcgaag
    10801 tataggtatg ggattactgt tttctatatg tattttttta ggatttacaa tatatataac
    10861 taaaagactt tcatatctta gttacgtaat agaaggtaca gaaagaataa aaaagggaga
    10921 attagattat aagataaaaa ttactgggaa tgataatttt acgtctttag ctgaaaatat
    10981 aaataatatt ggagaaggtc ttgatagagc tatagaagag caagtaaaaa gtgaaagaat
    11041 gaagtctgac cttataacaa atgtatctca tgatttgaaa actccactta cttctataat
    11101 aaattatgta gatttaatta aaaaagaaga atcaatacaa ccagagtata taaatgatta
    11161 tataaatgta cttgagtcaa agtcaaaaag actaaaatta ttgatagaag atttatttga
    11221 agcgagtaga gtaagtagtg gcaatataga attacaaatt agcaagattg atttagtgca
    11281 actattaaga caaagtatag gtgaacttga agaaaaatta tctaaaaata atttatattt
    11341 aaaattaaat gttccaaatg ataaggtgta tatatgggca gatggaagaa gaatgtatag
    11401 agtatttgaa aatcttttat ctaatatagc aaaatattcc ttagaagaaa ctagagtcta
    11461 tattgatgta tatgactatg gtgaaaatgt aaaggtaact atgaaaaata tatcttctta
    11521 tgaattaaat tttgacccaa gtgagattat ggaaagattt aaaagagctg atgaatcaag
    11581 gaatactgaa ggaagtggat tagggcttgc tattgcaaga gatttagtag ctttacaagg
    11641 tggaaaattt tatatagaaa tagatggaga tttatttaaa gcaaatttag agtttcaaaa
    11701 atatgaagaa gaaataacaa atatggaatg agatagtatt taagtatata tatttttttt
    11761 actgtcgaaa gcttttatta taatatatta gaatatttac agtagagaat gaagagtaaa
    11821 aacagagtaa ctttaagtca ctctgttttt tatttaaaat caataatata acaacttaaa
    11881 ttaaaattgt acacatagtt ttaaaataac ttatacaata aaacattatc caaatatcac
    11941 aagataattc caaataaata tatagatatc actcctgtaa taatagaaat accaatagat
    12001 ttaaacttat atgcaacaag aagtacaatc ataaaagata taattttagg gttagctata
    12061 gataaatata gactatctcc attaaaaaat acatctttag cagttaagga agtaagtatt
    12121 gctacaggta taaatttcat gatttttttc aaaaaatcag gcatctcttt ttttgaaaaa
    12181 attaacatag gaagcactct tggtagatat gtaacaatgt acattccaat tataataaaa
    12241 aatatataat tattattcat aagattcctc ctttttacag taagattctt ctataaagaa
    12301 accaattaaa gatgctaaaa ttgatgcaac tattattact aaattgcttt tgaataatgc
    12361 atataaaata acagaaataa tgattgatga tataaatact acaatataaa tcttattttt
    12421 tatttgagaa actattaaag ctataaacat agaagtcaat atgaagccag aaatagaact
    12481 gtctatagat atactttctc ctaaaaatgc tccaataaaa ttagctaaaa ctgatgaaat
    12541 taagcagaag aaattaagat aaatagcatt tctatcagac caatttccag cagtaaattt
    12601 ttcataatta actgcaaaag tttcatctgt tatagtatga caaaatggca atataaattt
    12661 tggactttta ttttttatgt acatgttcaa attagaactc attagaaaat gtcttaagtt
    12721 aactataaat gtagtcaaaa caatagatgt tacacttgca ccagtagaaa tcattgaagc
    12781 tgcaatgaat tgagaacttc ctccaaatac taaagttacc attagtgcta tttgaaaagg
    12841 tgaaagacca gatttttgta atagtattcc tcctactata cctataggta tataacctaa
    12901 gaatatagga attgaagcaa taaagctttc ttttaattgt tgagttcttt ccacaataaa
    12961 tcactccttt tgtattaaat gtattaaagt attagtaaaa cagaaaaata gattaattgt
    13021 ataattgcaa ttataattaa aaataaattt ggattaatgt ataaaatact atacaacaaa
    13081 taaataatct taaacggtta ttaaatttta tctatttgtt attatgatgt aataatacat
    13141 attaatctct ataagttaat tactatctgg tgtaatttta ttttaacaa
//
LOCUS       DAMJYC010000107        13091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJYC010000107 DAMJYC010000000
VERSION     DAMJYC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.111"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            96..725
                     /locus_tag="PC472_003266"
     CDS             96..725
                     /locus_tag="PC472_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728569.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            700..1104
                     /locus_tag="PC472_003267"
     CDS             700..1104
                     /locus_tag="PC472_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2728570.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1107..1580
                     /locus_tag="PC472_003268"
     CDS             1107..1580
                     /locus_tag="PC472_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2728571.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            2127..2792
                     /locus_tag="PC472_003269"
     CDS             2127..2792
                     /locus_tag="PC472_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2728572.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            2814..3746
                     /locus_tag="PC472_003270"
     CDS             2814..3746
                     /locus_tag="PC472_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2728573.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3767..4408
                     /gene="pcp"
                     /locus_tag="PC472_003271"
     CDS             3767..4408
                     /gene="pcp"
                     /locus_tag="PC472_003271"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2728574.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4528..4860)
                     /locus_tag="PC472_003272"
     CDS             complement(4528..4860)
                     /locus_tag="PC472_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728575.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5125..5745
                     /locus_tag="PC472_003273"
     CDS             5125..5745
                     /locus_tag="PC472_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2728576.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6070..6894)
                     /locus_tag="PC472_003274"
     CDS             complement(6070..6894)
                     /locus_tag="PC472_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2728577.1"
                     /translation="MDFLKKGISGFTIKILALIFMTFDHIAVFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6905..8308)
                     /locus_tag="PC472_003275"
     CDS             complement(6905..8308)
                     /locus_tag="PC472_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728578.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NTPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(8312..9037)
                     /locus_tag="PC472_003276"
     CDS             complement(8312..9037)
                     /locus_tag="PC472_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728579.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKVREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(9149..9922)
                     /locus_tag="PC472_003277"
     CDS             complement(9149..9922)
                     /locus_tag="PC472_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728580.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(9928..10815)
                     /locus_tag="PC472_003278"
     CDS             complement(9928..10815)
                     /locus_tag="PC472_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728581.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQSVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(10829..11851)
                     /locus_tag="PC472_003279"
     CDS             complement(10829..11851)
                     /locus_tag="PC472_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728582.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            12426..12602
                     /locus_tag="PC472_003280"
     CDS             12426..12602
                     /locus_tag="PC472_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728583.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4809 a         1891 c         1523 g         4868 t
ORIGIN      
        1 cttatattat ttaataattt attgacagta ttaaaattaa attatacaat aacagtgtaa
       61 attaacttaa caccgttaag taaagaggtg attatatgag tatcaaagaa agacgtaaaa
      121 atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt ataaaccaac
      181 atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat tctccaacta
      241 caatttatct ttactacaaa gacaaagcag agattatcag cgatatgacc aataaacttt
      301 ataacacagt agaaagtaat gctattgaca ttatgaataa ttgctcatca ctaccaatag
      361 ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt gaaccagaga
      421 tggcaaaagc tatcatgcat agtagaatga atattatatt tgcatctgaa aatacaaaca
      481 atacgcctag caataatggt ataaaaatgc ttgataaatt cctttccatt ggaattgaac
      541 aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt gcattgttag
      601 gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt tctcaactta
      661 tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg caacaaggct
      721 actaataaaa agaaaaaata tgtaaaaata tctattgctc tattctttgt atttatatgc
      781 attattattg taattgtaag caacttaaag ccagaaaact tggtagtaaa agatatagat
      841 attaataatg ttaaaaatgg catttatact ggaagtgctg ataataactt ggtaaaagct
      901 actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt aaaacacgac
      961 catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca acagtcttta
     1021 gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa agctgtagaa
     1081 aatgctttaa gaaagggaga ataattatga atactattat tatatattca agtaaatatg
     1141 gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat gtcactattg
     1201 ttgatattaa taataataat aaaattgagt tatcaaaatt tgataaaatt attattggaa
     1261 gttctatata tgttggttca gtttcaaaaa aaatacaagt gctatgtaat gataatgtag
     1321 agttattaaa caaaaaacaa gttggcattt tcctttgctg tggcttttct gaacaagcag
     1381 ataaatattt aaaatcaaat tttccatcat ccttattaga aagtgcaaat gctattggaa
     1441 tttttggtag tgaagctcga ttagaaaaaa tgaaattttt agataaactt attatgaagg
     1501 ctgtttcaaa aggtaattat gacagtttta gaatatcaca ggataatata gataattttt
     1561 taataaatct taatagctaa aataaagcta attatttcta tgataaattc ttttatttat
     1621 aagtaatatt gctcaaaata gtctttaaac tttatcaaaa ttttattttt cttagtgaaa
     1681 tctaatgaaa ctagcactta atatatgttt tattaatcat aacaaaaaga gagtgtctaa
     1741 tgaactaaaa aattcattag acactctctt tttgtatgaa atcaaatata tttttatcat
     1801 ttcaaaagag aaaaatacac ttgtctctat gtttggcaca ttctcttttt ataatataat
     1861 atctaattta aaacctattt caaatattga catatactta aactatagac ttacaaaatc
     1921 aattaatatt agcttaaaat ataatctttg ttattcaaat tcctatacac aaccattatt
     1981 tactttgata ctttaaaaca tcttccctat tttttattaa attttataat aatagtccta
     2041 ttaacgcaaa tactataaaa agagtttaac tttatccatt aaataaattt aaattaaaac
     2101 atatagaaat ggaaggagaa caatttatga taaaactaat cggtatattg atagttgtaa
     2161 ttggatttat acttaaaatt gacacattat ttactgtatt attagcagga gtggcaacag
     2221 gaattgttgc aggattagat tttaatcaaa tttttacaat tttaggagat tcttttgtct
     2281 caaacagagg tgtatcatta ttcatattaa cattgccagt aatcggtgta ttagaaagat
     2341 atggtttaaa acaaagagct gtttctttaa tagaaaaatt aaaaagactt acaacaggaa
     2401 aagtacttac tatatacatg atagctagac aaatagctgg tgctctatca ataagaatga
     2461 gtggacatcc tcaatttgta agaccacttg ttaatcctat ggcacaagct gctggtttaa
     2521 gtaatagtga tgaactaaaa gaatcggatg aagaagccat aaaggcatta tctgctgcat
     2581 cagaaaacta tggaaatttt tatggtcaaa acttatttgc aggtagtagt ggagtattac
     2641 ttatagcatc tactctgact caatttggat acaatgtaac tggacttaat atcgtaaagg
     2701 ctagtactat tatggcagta atagcacttg tagtggctac aatccagttt accttatatg
     2761 ataaaaaatt aaacaaaagt cataaaaaat agttttattt aggaggaata aaaatgcaaa
     2821 attttataaa catatcattg gaaatctttt acgcactaat gggattttta atgattgtta
     2881 tagcatataa gtcttttact accataaata acaataagaa atatggtaca tcattatttt
     2941 ggatacttat atcattgcca ttcatatttg gtagattgat tccagccaat ataataggaa
     3001 taatattaat attatctagt ttattaacac tttctaaaca agttgtattt gcaaagtatg
     3061 aagaaccaga tgaaaatttt ggaaaagaac aagcagataa acttaaaaat aaaatattta
     3121 taccatcatt aatattagca tttgctgcag tagtagtagc tatgagcctt tctaattttg
     3181 ataattcttc tcaatttgca ataggtgtgg gttcaataat tgcattaata tcagccctta
     3241 taataactaa ggcaaaacct gctacatcag ttcaagatgg ctcaagatta cttcaacaaa
     3301 tgggacctgc tagtatgtta ccacaacttt tagttgcatt gggagcttta tttactcaag
     3361 ccggagtcgg tgaagttatt tcaaccatga tatctggtgt agtccctgct gatagcagat
     3421 tatttggtgt tatagcttat gtattaggta tggttatatt taccatgata atgggaaatg
     3481 catttgccgc attttcagtc ataacggcag gaattggtat tccttttgta ttgtctcaag
     3541 gtggaaatcc tgctataatt ggagcattag cattaacagc aggatattgt ggaactctac
     3601 ttactccaat ggctgctaat tttaatatag ttccagcagc cttgctagaa tgtaaaaatg
     3661 attatattgt tataaaatat caagctccag ttgctttggt attaattata gcccacatat
     3721 tagtaatgta ctttttagga ttttaatata attaggaggt aaaataatga aaattttgtt
     3781 aacaggattt gacccatttg gtggagaacc aataaaccca gctcaagaag cagttgaaag
     3841 ggtcaataat aatataaacg gtgcagaaat cataaagata actataccaa cagttatgac
     3901 aaaatctgtt gaagctatag ataaagctat tcaagagcac aatccagata tagtaatatc
     3961 agttggacaa gctggaggta gatttgacat tacacctgag agagttgcta taaatataga
     4021 tgattttaga ataaaagata atgagggtaa ccaagttata gatactatta taaaagaaga
     4081 tggtgaacct gcatattttt caaaattgcc tgtaaaagct atggttaaac atatgaatga
     4141 aaataaaatt ccagctagtg tttcaaacac tgcaggaaca tttgtttgca atcatgtgat
     4201 gtatggaatt ctttatatga tagacaagaa atatcctaat ataaggggtg gatttataca
     4261 catcccatat acaacttcac aagtaataga taaaaaaaat acaccattca tgtctttaga
     4321 agaaatagta aaaggcttag aattagctat agaagcttgt attatatata aagaagatgt
     4381 aaaagaaatt ggtggtgaaa tttcataata aattttttag tatttttata aattaaaaat
     4441 actaactatt aatttaaata tagactgtga gtagattatt caaacaattt tactcacagt
     4501 ctttttatgg tatctacaga tacaaaatta aggtctattc ctctctcccc actcacacat
     4561 tgcatctaaa attggaatca gagattttcc cctttctgat agactatatt ctaccttagg
     4621 aggtatttga ggatattcag tacgaataat caagttatct gcttctaatt cttttaatga
     4681 taaactcaaa gttttatagg agatagtatt tatataacgt tttaatgcat tatatcttac
     4741 aacttcaaat tctacaagac agtaaagaat tatcatctta tatttaccac taatcagtga
     4801 aagtgtataa ctaaatcctg tttcacttaa atctgtattt gatatacagt ctatactcat
     4861 atattacact ctccttttgg ttagtattga acaaaaatgt gcgtacttgt ttttagaata
     4921 atttataata aaattatagt gtaagaataa ttgaaagtca attttataat aggaggtgta
     4981 tttactaaaa aaaagttatt ttaaatagga ttctaagatt agacatttct atataaattg
     5041 ataatattca tgaaaatcct attttataaa aaataatgtc tcttagaaaa tctattaaat
     5101 aaaattcatt ggaggaaatt tataatgaaa gttttattga taaacggtag cccaaaccaa
     5161 tatggatgta cttatacagc actaaatgag attactaaag tattgagtaa acacgatatt
     5221 aaaacagaaa tactgtattt aggtaaagaa acaatcccag gatgtattag ttgcgcatcc
     5281 tgttttgaaa ctggaaaatg tattcgaaat gataaagtta atgaattgat tgaagatctg
     5341 gataatattg atggtattat tattggttct cctgtttact tttctagtgc aactggtcaa
     5401 ttaacttctt ttcttgaccg tttatttttc atagctggga gtcgcatggc tacaaaatta
     5461 ggtgcttcag ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga ccaactaaat
     5521 aaatattttt caatcagcaa tatgcctatt gtatcatcac agtattggaa ccaggtacat
     5581 ggctttactc cagaggatgt tatgaaggat gaagaaggat tacagactat gcgaactcta
     5641 ggagaaaaca tggcatggct tcttaagtgt atatcagcag gtaaaaaagc tggaattaaa
     5701 gaaccacaat atgaagaaac aattatgact aattttataa aataaataaa aaatgcacta
     5761 atattaatta aaacaaaaca ctaatttata aataaaataa gcataaatgt aggataaaaa
     5821 gcactaaaac agaggtattg aattttatat caaacctctg ttttattatt acattaataa
     5881 ccaaaccata tataacatca tatatttcta aataaattat aatattatat tctaatgtta
     5941 ttgaaattac aataactgaa tttctgctca taaatttaaa tattctacaa atctcatcta
     6001 aataacaatt ttaatagacc taaaacaatc ctcatattgt tcattttgta ttaaaaatat
     6061 cacttacaat caaaaaccat ttattaagta tattcctaag atatacaata tatatacatg
     6121 gattggatag tataaataaa ataaatactt catactcttc cctttttctc cattgtaaag
     6181 taacattaaa ggtagagaag caatcatcat ccattggtaa ttttgtaaaa tagaattttt
     6241 taaactataa tctccatttg acattgttgt aaatataaat atacacatta aaacataaca
     6301 aatactcaaa ctgaactttt tacctctaca caaataaaaa ataatcccaa gtataatcca
     6361 aattggtcca ccttctacta aaaatggtac tggtaccaaa atcataaata aagcaataac
     6421 tctgttagtg actttaaaaa gtaaagctac tgtaaatatt cctaaaatta taggaatgat
     6481 tatcatcaat aatcctataa taaaatattt aatttctttc tcttttctaa atttctttat
     6541 gaactctatt gcttgtaaat aaatcgctat taaaaataat gttgaaaaaa tgttatttat
     6601 aattatagct ccttctggat gtgaaaatac attattaatt atttgatttc ctatagccat
     6661 aataacagac caaatataca atctacttat atattttttt ctgttactag tgtgataaaa
     6721 tccttcaaca gccatgaata taaacagtgg tgctgatatt ctgcctaccc aatgaaacca
     6781 aattggtatt tgcattgttt gtggcataaa tactgctata tggtcaaatg tcataaatat
     6841 taatgctaaa atcttaatag taaacccact tatacctttt tttaaaaaat ccatatcata
     6901 tctcctaatt ttgttttatc tctataataa tttttgttcc tattccaatt tcactactta
     6961 cataaatttt acctttatga atctctatta tttctttaga aatagccata ccaagacctg
     7021 aacctttatg catctcacct gtattagtac ctctataata ttttttaaaa atatgtttca
     7081 aatcactttt gcttattcca attccattat cttttattat aatttctata ttatcttttt
     7141 ttacaatttc tacacttatc aaagttcctt cgctattatg aactattgaa ttaaatataa
     7201 gattggttat agcccttctg aataagattg aatctacata tacttcaatt aaatcttcat
     7261 tactttcaaa ttcaatattc ctatttttat atctattatc atttaaaata tcaattatta
     7321 tatctctgac taaactgact aaatttattt ttttcttgtc cagaactata gtattatttt
     7381 ttaatcgagt agataaattt aaatcatcaa ctaaatcttt tatatactta gacttattat
     7441 atataatttc tgtatactct tgtatttcat cttcttcaaa ctcataatct ttgtcattaa
     7501 ttatctctgc atacccttgt atagatgcta gaggtgtctt tatatcatgt gttatatttg
     7561 ccagccattc ctctctcaac tcttcatttt cttttctctc aacttcattt actcttaaag
     7621 tatcagctaa catatttata tttttaaaaa cttcttcata tatacctctg tccttaagat
     7681 ataaactata atttccatta gccatagtct caatactcca tagtacgctt gaaattggtt
     7741 ttgtaagttt tctactaaat aaatatccta cacctaaagc taccacgcta tcaattacta
     7801 gtgttataat gataacttta tttaaagttt ttttaatgtt attttgagaa taggttaaaa
     7861 ttattcttga tatatctcta ctaggtatac ctactattat gtttagatgt tttttgttta
     7921 aatatttttc tagtacaaaa ttaacatact ttgtttctat atacttgtaa ttattcacca
     7981 tttgaaaggg tgtatatttt ttgggagtat cctttggcac atttactcca taaacttcct
     8041 ctcctaaatc atttaaaacc tgtatccagg aatttgattt ttctaatttt tcagctccaa
     8101 tcttagatag tttaaattct ccatcttttt catataaatc tttttcaaaa gaacgtgcaa
     8161 attcttcagg agtatttcca gaatctacaa ctttaaaaaa actattagtt gaaatcacat
     8221 acaatataga tatgatattt ataattacta ctgatatagc tacaaacaca attgtaaata
     8281 taaaatttct agtaatcttc cacttcatat tctaatcttc ctttactgct aatttatatc
     8341 caagaccttt tattgtcaga atatattcag gctttgaagg attatcctct aatttttccc
     8401 taacttttct tatatgaacc attatagtat tgtcaaatcc aacataatct tctccccaca
     8461 ctttatccca tagtttttct ttacttataa tttgattttg attatgtgca aataaagata
     8521 ggatttttag ttcctttgca gacagtccta tagattttcc atcttttata agttctgccc
     8581 tactttcatt taatatatat gggccaaatg atataatttt ttcttcatta ttatttagat
     8641 ttttactttc gttttgagca tcactaaaat aacttaatct tctcaaatgc gctttaactc
     8701 taaaagccac ttccttaggg ctaaaaggtt ttgtaacata atcatctcct ccaatcgcta
     8761 gtccaagtaa cttatctaat tcttcggttt tagctgataa aaatattatt ggagaattgg
     8821 aggtttttct tatttcttta caaatatcat aaccttcgcc atctggtaac ataatatcca
     8881 atacaatcaa atcaggatta atacttttaa ataattctaa tccttctttt ttagtttttg
     8941 cagtatatat attattaagg ccctcttttt ttaggacagt ttctaaaagc ttcaatattt
     9001 ctacttcatc atctattatt aaaacacgat tttccataaa caacctcctg taataattta
     9061 catatatatt agcatatatt ttatctaaaa ttcatttatc ttatttttaa ttaaactaaa
     9121 atacaaataa aattactatt atttttggtt aaaacctttt gcttgatgta aaattttcat
     9181 acgttatttt agttaacact atcattaata taattgagat tactatagaa aaaactatca
     9241 aaatacttgg tacattatac ttaaccattt gttggctcat cataatccaa tttggagtca
     9301 ctgacataaa ttttatagct ttatctggta taaaacctac actaaagtat ggaattgcca
     9361 ttatcaatcc acctagtata aatggaatta cagccgattt aaatatagat gaaagtgtta
     9421 agcctatttg tgcaaatata aaagaagcaa aaatagtagt caatataaca attatataca
     9481 tttgtattac tgtaagattt aaattactat ttccaaagta ccataagtta tgaatcgatt
     9541 catttaaatt tttaaaatca aaaaaactaa aacctctaat tcctactaac aatataaaag
     9601 aaatattaat aatagataaa attaatccat tagctactag ttttgataat actactgatt
     9661 tttttccctt tttagttgta tttattaaac ctgtcatatt agaatcatat tctttaatgt
     9721 aatttccaga agttattata aacattatca aacctattag aggtattgag ccttctatct
     9781 tgtaaaatat atctgcataa gtatctaaaa catcaaactt tctaatcaga aaaaatccaa
     9841 aaaatattaa aaacagccaa attatccaaa cactctttga ttttaatatt tttatcattt
     9901 cagctttaac taaatacatc atttctacta cacctccctt gcatcttcca aatcaaaata
     9961 aaacatgtat acatcttcga attttgcttg aactgattgt atattatccc cacttggaat
    10021 atcctctgaa acaattctaa gtactactga atcaatatca ctttgaatac ttacaacctt
    10081 atatttagat tgtatatcat caacttcaca ttcattttta acttgaacat tatatacttt
    10141 attttccatg tgacttaata tgtctttgtg tgttcctttc attagaagtt tgccatcttt
    10201 aatcattatt gtttcctttg caactgattc tatatcggaa attatatgag tagaaagtat
    10261 tattatctta tctctagaca tttttgctat taaattccta aatctagctc tttcttgtgg
    10321 gtcaagacct gctgttggtt catcaaggac tattatctta gggtcgttta gtaaaacttg
    10381 tgctatccca actctcctct tcatacctcc tgaaaatttt cctatagact tatttctcac
    10441 atcataaaga cctactaaat ctaataattc tcctatctta ctatttaaat attttcccct
    10501 taaacctttt aaagctccaa tatactgtaa aaattctttt gctgtaaaat ttttataaac
    10561 tccaaattct tgtggcaaat atcctaaaat atttctataa tcagcatcca ttttaaatat
    10621 atcctcacca ttataaagta tctgaccttc atcaggtttt atcaacgttg ttatttgttt
    10681 cattaatgta gattttcctg ctccatttgg tcctagaaga ccataaactc cattttccag
    10741 agttactgta atcttgtcgt ttgctacttt tttaccatat atctttgaaa gattttctat
    10801 ttctaattta ttcatattaa ctcccctttt atctacttat ataatctgat tttattacta
    10861 aaaaacataa tgacatagca cctatcaata ttgccatcaa aagtcctatt gctatattga
    10921 caatgtctat attcacttta aatatcataa tatcacctag ataatttcct attattttat
    10981 taaaaccaaa tatcaaatca taataattta catttgatac aactgaaagt aattttttag
    11041 gtaatatatg gattaccgac attatcttag ttatagatat aataaatata aaaaaactta
    11101 ttgaagatat tgaagactta cttataaaag acccaaatat ggctatcatg gcccatcctt
    11161 caaacatcaa aagctctatg cctatattta atattacata ctttgttata cttaaattag
    11221 ttttcaatgc aactacattg tctactattc taaaagattc taagtctcca ttaacaggta
    11281 ctccttgttg catacaagtt ataataaata ttcccattaa ataaacagaa taaattacta
    11341 atggaactaa taaagcaaca aatattttag actttaatgc ttttttcttc tcttttgatg
    11401 atagtatgat atcttttacc gaagtaacga cttcatctac ataaatatta gatataatta
    11461 tagatataat gattaacatt cctacattta taagtgaatt tgtaactcta tagttaaata
    11521 ctttccaaaa actaatatcc ttatattcat tcacacttag attatcaagt tttttctttg
    11581 acattaattc aattttttta gaaaattcac ctgtatctgt ttgatttgaa aactgcttta
    11641 aaacattaat cttattttga aatttctcat tacctatgtc tataccactt ctcgtatctt
    11701 ctatacgtcc tttttcttta tctatataat aactttctgt ttctaaaaca ggcgtaataa
    11761 agatactaat cattagtatt aacactaata tccctagaga tataaacata ctcttagatt
    11821 tagccaattt ttttatttcc caatttacca atatatttct cctcctaaat cattttaatt
    11881 acacatatat attaaaactt aaatcttata taaatataat actaacctta aataaagctt
    11941 aaattatttt ataatttata ggtgtgttta ttattggagt tacatttatt atttttctgg
    12001 gaaattatac attttcaatt atttttatta tttttgatat ttttattgat tggaaatttt
    12061 attgttgttc aaattgtagt tattctttaa atttaaagat ggagtttttt aagaatagaa
    12121 cctactgtcc ttattatgga aagattatta aatagttgca tataacttta tttatcttga
    12181 atcttatttt taaaaacaat tataatttta gaatattttc tattctcaaa caagcatgtt
    12241 gaataatgga gcatcttcta gtattatata cttgtagaat aaaactaaat tggtaaaact
    12301 gggaatattt aaccttaagg agttaagtta tagaaactag tactaaaaaa ttttataaat
    12361 tatgttaatt gactaataga atgcgacttt ttatttttac taaatttttt agggggatgg
    12421 ataatatgtt tattggagaa atagacaaat gtacacatat actaactgct tacatcagta
    12481 gttcatatga ttattgtaat tttttagata cacagttaga tgattttata tcagaatatg
    12541 gagaaacggt agttgaaacc tgcttacatc aagtcttatt attagtaagt agatacaatt
    12601 aaaactaaat ttttctagaa aaaccttttt agatttatct actctaataa atatataatt
    12661 ttttgtactt tataagtaac tataacttct gctaaagtaa agatgtattg ggagtacatc
    12721 tttactttcc cattatagaa gttaaaagca tacaccataa aattaatcat ccaatacaaa
    12781 ttatatattt cctatgtcta acttaattta caagaatcat tctatatgca tattctgcaa
    12841 tccactaaac atggctctaa acctttacta ttttatacat tctctataaa tcatacaaat
    12901 catatataaa atccccctaa tatgtttaaa ccacctataa aataacacca atatttagaa
    12961 tattcaatat caactgcata actggtcaat aaaaacgtat aactagacaa atttcaatat
    13021 ttatataaat aatacaagaa aaacaagcat tttatttata taagtttttc attttttgtt
    13081 tatattaaaa a
//
LOCUS       DAMJYC010000108        12822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJYC010000108 DAMJYC010000000
VERSION     DAMJYC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.112"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            113..301
                     /locus_tag="PC472_003281"
     CDS             113..301
                     /locus_tag="PC472_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2728584.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(461..613)
                     /locus_tag="PC472_003282"
     CDS             complement(461..613)
                     /locus_tag="PC472_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2728585.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(711..1187)
                     /locus_tag="PC472_003283"
     CDS             complement(711..1187)
                     /locus_tag="PC472_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2728586.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1406..2167
                     /locus_tag="PC472_003284"
     CDS             1406..2167
                     /locus_tag="PC472_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728587.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2427..4355)
                     /locus_tag="PC472_003285"
     CDS             complement(2427..4355)
                     /locus_tag="PC472_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2728588.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4589..5041
                     /locus_tag="PC472_003286"
     CDS             4589..5041
                     /locus_tag="PC472_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2728589.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5044..5352
                     /locus_tag="PC472_003287"
     CDS             5044..5352
                     /locus_tag="PC472_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2728590.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5466..6572
                     /locus_tag="PC472_003288"
     CDS             5466..6572
                     /locus_tag="PC472_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2728591.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENIEFDEIELDIM"
     gene            6647..9325
                     /gene="mngB"
                     /locus_tag="PC472_003289"
     CDS             6647..9325
                     /gene="mngB"
                     /locus_tag="PC472_003289"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2728592.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9642..11975)
                     /locus_tag="PC472_003290"
     CDS             complement(9642..11975)
                     /locus_tag="PC472_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2728593.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKAISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTMTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11950..12666)
                     /locus_tag="PC472_003291"
     CDS             complement(11950..12666)
                     /locus_tag="PC472_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728594.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4777 a         1791 c         1672 g         4582 t
ORIGIN      
        1 ttcaatatgt gtaaggcaaa tatatatttc tattgcaaat ttttgttatt tatgataata
       61 taaatatgtt tttagtcttt aaatagttat cattttaagg aggtgtgcgt agatggcaaa
      121 agtatgtagc gtatgtggta aaggtaaggt ttctggaaac caagtatcac actcaaataa
      181 acataataaa agaacatggt cagctaactt aagaagcgta agagcgatta tagatggagc
      241 tcctaagaga gtaaaagttt gtactagatg tttacgttct ggtaaaattg aaagagctta
      301 gtaaccacgt ttaaaagcct acttccgtaa gtaggctttt taatttaatc atttttactc
      361 tttctcataa attttaggat tatacttgaa atccattttg gtaattttat tgttactact
      421 ttcatcaata ctcaccccac actttatagt taaaactatt ttaacaaaaa aataataatg
      481 ctccacatcc aataagtatt aatgcggtaa gtgctgacca aatccagcca ggaagcaaca
      541 ttgacagaat tacagccatt cctgttgcta gacaaagtat tcctagcaat accttagact
      601 tgttatttct catggaacac ctccacatag aacctgtatc tataaatatt ttcatattca
      661 agtttatgcg acagttttcc attttagacc aataaacaca ttatgtaatt ttaatttttc
      721 atactagaat tattactgaa ctccgatata gattctacaa tatccaaaat tccttttttt
      781 ctacttaacc tttcaggtcc actatataaa acaactggta taactttagg aagtttaaaa
      841 tttttatctt ctatttcttc aaggcttttt ttcataataa aacttttcca aatttgttct
      901 ctatacgatt tcaatcttgg gacaatttca gtatccattt ctgtttgaaa ttctaacact
      961 atataatgtg atactttgcc gcttttagaa aataccttat aaattatatc aacttctttt
     1021 cctttatatt cacatataaa agatgtttct aactctatat ttttaagagt taattctttt
     1081 gctattgaga cacttgtaaa attttgaatc aaacttaaaa aactttcttt atctgaatac
     1141 attcttctgt actcttctcg tttttcattg cttcttttta aattcataca gttcctccta
     1201 gactatagac cctctatttt ttagtaaaga tagtctataa attttatttt ttaattttca
     1261 ttagtgagta ttgacagttg tgtgtaaaat atccagtaaa gatatttttt tatattttta
     1321 ttaaaagata taaattttta tattttaatg tattgacatt tacgttgcgt aaaagtgtat
     1381 atttaattta taggaggtac tacttatgga atatacagtt caaaaattaa gtaaactagc
     1441 tggtataagt actagaacac ttagatatta tgatgaaata ggtcttctta aaccacttaa
     1501 aataaattct tcaggttatc gtatatatgg tcaaaatgaa gttaataaac ttcaacaaat
     1561 acttttttat agagaattag gaattaactt agaaaatata aaaaacatta taaattcacc
     1621 aaacttcgat agtttaagtg cgttaaaaga acaccataat aaattactag ccaaaagaaa
     1681 gcaaattgat ttactaatag aaaatgtaac taaaactata gctctgaagg agggaaaata
     1741 cactatgaca gatatagaaa aatttgaagg ttttaaagaa aagatgattg atgaaaatga
     1801 aaaaaattat ggaactgaaa ttagagagaa atatggtaaa gatattatag atacatcaaa
     1861 caaaaaactt aaaaatatgt ctaagcaaga ttatgaaaat tggcaaaacc taaatatcga
     1921 aataattagt aaacttaaaa aagcttttaa aactggtgat gcctctagtg aactggctca
     1981 ggaagttgca aaattacatc ataaatggtt gagttacact tggaacacat actctaaaga
     2041 agctcattcc tctctagcac agatgtatgt atatgatgaa cgttttacat catattatga
     2101 tagagaacaa cctggattag ctaagttttt aagggatgct atagtaatat atacaagaga
     2161 taaataatat aaaataataa aggtggtgtc tcaaaatgaa ctttttagtt catgagacac
     2221 tcttttttgt atgaaatcag atatattttt atcattttat caatgaaaaa caggtttctc
     2281 tctctatttt gagacagaat ctttattgta taataattat aatcaataca aaataataac
     2341 aaattataca aaacttactt atactattta tgctaaaaac aaaaaatatt ttattaaagt
     2401 ttttaaaact tatgtaatat aaaaatctat actatagact tgattatgga aagaaaatta
     2461 tcaaaatcat tacaattaga tatactatct ctaatatcat tgtcatgaat tatctttaaa
     2521 aggatatcat aaatggagtt tatttttata taatcatctt tatttatagc caacatcatg
     2581 attaatttaa cagtagaatc ttcactccaa tatattggtt catccaatat ggaaatacaa
     2641 atcttagttc ttatagagct taattccata ggatgaggta tagctaaaaa atcatctata
     2701 ttggtagaag ataaactttc tctgtaaaat acagattcag caaatgatgg aaatacaatt
     2761 tcactttgag ttaatttgtt gcacatcaac tttataagct catctttatc tttgatttta
     2821 ggattaacaa taaataaatt tttatcaaag aaattgtcca aaagattact ataaatatgg
     2881 gaattattag tgattgactt agatatattc tctatatctt tttttaatag ttttaaatcc
     2941 actaatacaa taggaatact atcatgattt aaaggaattg tcgatattat tatatctaca
     3001 tctgacaatt cactagctaa aaattgatta aatgatagac attgaagtat atttatttta
     3061 tcatgaaaaa ctttgtttaa ttgaacctct aataacctac ttgaaccata ccctgaacca
     3121 catactaata ctacgttttt acacttaata ttactttgaa aaaatctctc tattccagca
     3181 cctatatgca gggatacata tccaatttca tcttctgtta aagagtaaat actattttta
     3241 aaaacttttt ctatagcatt taaagttatt tcaaatgcta aaggataatt actctttatt
     3301 gtatttataa ggggattcac cttatttaaa ttataagatt tagaattgag aattgattta
     3361 aagtgcagta ctaaatcatc aaataataca ctatcatttc taaggtcaaa agtatattga
     3421 ttgttgatta cttctaaaat ttcttcaaca tactctataa ttttagtatc taccctattg
     3481 gaaacgttat ccaaaagaga acttttagtt ataaaatgat tatatagata aactctgtct
     3541 gcctttgaaa taattatagt atatttgctc tcaatataat taaatatatt ttcaacagtt
     3601 gtattcgatt gaaaatctgt taaaacaaca tctaaaacat tatctaagca gtaaccatca
     3661 agtattctac ttacagtaat agctaaatgg ataataaagt tttttagatt aaaatctagg
     3721 aaattgatat taaatttatt aatttcactt aacactaccc tttgaagctc tattaaatct
     3781 acattattaa ataattctat ttctctatct gtaattccaa ttatatagtt ttcaaaattc
     3841 ctatttatca tattattaga aatacatctt cgaatatcta tttccttccc ttcaacttta
     3901 actccataat atggcttaga aactaatatt aaattatact ttgacaaaat aggctttatt
     3961 ctttttatgt catttagtat tgtaaattta ctcacataca agctattagc taaatcttct
     4021 agttttatat atgtattgtt atagagaata tattttaaaa tatattgatt tctttctata
     4081 ggagaatctg gtatttcact tgaatccata atatcatcag atattaatgc tagatactgt
     4141 tggtataatg ctaaattaag aatatctata taatatcctt ctcctttttt tattttaata
     4201 atagcaccat ttttctctaa agttccattt atagaagtaa tatcatttct tatagttctc
     4261 tcactcactt ttaaatgatt acttaaagtt ttacaagtaa ttttatcatc actttcattt
     4321 aataatttaa acaacgtgag caatctcttg tgcataaata tcccccctta tagaatttct
     4381 tctaaattat actatttaca ctaatataat acaattactc taataaaatt gtattataat
     4441 aatttatttc cacttctata tggaaattag ttattatcat aatataaatt tccatgtcga
     4501 agtggaaatt agttatattt aatttaatta ggataatgat tacaatataa atataagtta
     4561 agacattatg aatggagtgg aaaaatatat ggtactacaa gaaaaatata tatttataga
     4621 taaagactta aaaagcaaag aagaagtatt aaattttatt gcagatagtg cttatatact
     4681 aggtattaca actaataaag aggctctttt aaaagacctt ctaaaaagag aaaatgaatt
     4741 tccaacagga ataaaagatg gatttgcgat acctcataca aagagtgtaa atgtaaagga
     4801 agcttccata ctctatatta aaacaactaa cgagttagag tggaaaactt tagatgatag
     4861 taaggttaga tatgtattta gtttgttagc cccacataaa aatgaaggaa atatacattt
     4921 gaaaactcta tctaaattag ccacatgttt aatggaggat gattttacaa gtgaagttaa
     4981 aaatgctagt gatgcaagtc atttagtaaa atatataact aataaaatga aggaggaata
     5041 attatgaaaa tagtagcaat aacatcatgt atagcaggat tagcacacac acctatggca
     5101 gcaaaagctt tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt agaacaacaa
     5161 ggtgctatgg gacgagtaaa tactataaca aaagaagaag cacaagatgc ggactttata
     5221 cttatagcat ctgaccaaac tatatctgga atggaaagat ttgatggtaa aaaagtgata
     5281 aaagtcaaaa taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa gtgtatagaa
     5341 gctatttctt aataaaaaaa tctcactcta atatagaatg agaaaaatca aaaattaaaa
     5401 aattgtctaa tataattata acatattatc aaaaaatata acaaacttat ataggaggat
     5461 taaaaatgag taaaaaaaat aaatctcttt taagtgaact taagggacac ttaatgacag
     5521 gtatctcata tatgttgcca cttgtaatag gctcatcttt agttgtagct atcccaaagc
     5581 ttacagcttt agcagtgggt ataacaagtt tagatgctta taaaaatgta tctggttttt
     5641 atcatattct ttacttaatg gaacaagttg gttggactgg cataggtcta ttaaatacgg
     5701 tattagcagg atttatagca ttttcaatag gtgagaaacc agctatgggt gcaggtttta
     5761 ttggtgggct catagcaagt aatactaatg caggattctt aggagcagta atagcaggtt
     5821 tctttgctgg gtatgtttgt aagtttttaa agaaaaaaat taaaatatct gggtcagcat
     5881 caggaatgat gccacttata ataatgccac ttataacagt tggattaact ggttttttaa
     5941 tgagtgttgt attagcagga cctttaggaa acataaatac ttcattaaat gcttgggtag
     6001 ctgaaatgtg tcaaaatgga acaaattcgg tggtattggc tttaatttta ggcgcaatga
     6061 taggattcga tttaggtgga ccagtaaata aagcagcttg gatggcagga aatgcattat
     6121 tgttagaggg aatctatcta ccagcgatat tggtaaactg cgcaatagtt atacctccac
     6181 taggatatgg tttagctaca ttcataagaa aatcaaaatt ttccaatgca ttagctgaaa
     6241 caggaaaagg caatatagtt atgggtataa ttggaataac agaaggtgca attcctttca
     6301 ctttaacaaa tccattaaaa ttagttcctg ttaatatgat aggatgcgct ttgggtgtag
     6361 gattatcagc tttgttaggt gtacatgcta taatgcctcc agttggtgga ttatatggat
     6421 ttatatcaat aggttctgga tgggcttact tagtaggtgc aatatttgga gctttagtaa
     6481 tagcaatact atcaacaact ttagtagact tcaatgaaga tgaaactacg gaaaatatag
     6541 aatttgatga aatagagtta gatataatgt aaattaaaaa cttaatttta gataattgag
     6601 aattgagtgt actctcaatt ctcaattaaa ttttgggagg atttttatga aaaagacaaa
     6661 agtacatgta gttccacaca gtcattggga tagagaatgg tattttacaa catcaaggtc
     6721 aaaaatatat ttaatgaaag attttcaaga tatattagac atattggaag aaaaagaaga
     6781 ctttaaatac tttactatgg atgcacaagc ttctctctta gatgattatt taaaatggag
     6841 acctcaggat gaatatagaa taagagattt agttcaaaga agaagattag ttattggacc
     6901 ttggtatact caaacagacc aattggttat atcaggagaa tctataatta gaaaccttta
     6961 ctatggaata gatagatgca atgaatttgg tgaacctatg agggttggat atgtaccaga
     7021 ttcttttggg caatcagctc aaatgccaca aatatacaga ggttttggaa tagatagttc
     7081 attattttgg cgtggagttt ctgatgatat ggtagataca acagagttta tatggaaagg
     7141 ttctgatggt tcaaaagtat tagcagttca aataccattt ggttattact atggaggtaa
     7201 tataccagaa gatgattcag atttaaaaga atatttaaaa gatataatcg gaaaacttaa
     7261 ggttaaagct tctactaata acgtatattt tccaaatgga tttgaccaag caccaataag
     7321 aaaaaacctt cctgatatat taaaaaaagc taatgaaata gataatgaaa atgaatacga
     7381 aataagtgat atagaaaact atatagattc agtaagaaat gaaagaaatg aatttacaga
     7441 gcttaaaggt gaatttttaa atgctaaaca tatgagaata cacaaatcca tattctcctc
     7501 aaggtcagac cttaaaataa tgaataacaa attagaaaat tatattacaa atgtacttga
     7561 accattatta acattaagtt attcacttgg aaatgaatac cctcatctag ttataaaaga
     7621 tatatggaaa cttatgtttg aaaatgcagc tcatgacagc ataggttctt gtaatagtga
     7681 cacaacaaat gaggatgtat acacaagata caaacaagcc agagatttga gtataaattt
     7741 attagatttg catatgagac ttataagtac tcaaatcaaa aataataatg aagaaataac
     7801 attaacagta ttcaatggat tcccagaagt cagaaatgaa gttgtagaat ttgatactta
     7861 tattccaggt caagattttg tgataaagaa taaaaatgga gaaattctta attatataat
     7921 aaaagaaaaa gaagatatta caaattatat attaactcaa acaataagat taaacccaag
     7981 taaaaaaata tatataccca aaactgtata tagagcaaaa attgcacttt cagtaaaaga
     8041 tataccacca atgggatata ctcaactagt ttttgaattg gatactaaaa ataaatgtga
     8101 aatagaaaca agtaattgca aagaaataga aaacaaatac tacaaaataa cagccaataa
     8161 aaatggttca ttagaaatat tagataaaga atctaataag acttataaaa accaagctgt
     8221 aattgaagat aatggagatg atggagattc atacaactat tctcctccaa gaaaagatat
     8281 ttatgtttca tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa acattcaaga
     8341 agaaatgata cttaaatatt cattgaccat accagcttca ctggaagaaa gggctatagg
     8401 taaagtttct gtgaaaatgc cagtaacaat gaaaattacc ttagaaccta aagaacaaat
     8461 aattaagttt aatgttaata taaaaaataa ccaagcacta tctcatagag taagagtttt
     8521 atttaataca gaaatagcat ctaagttttc tatagcagat caacaatttg gaataataca
     8581 aagaccaaca gtattagaaa aagaccttgc attgtggaaa agagacaatt actcatggca
     8641 agaaaaacct ataactatcg aaccaatgca aagttttgtt actttagagg atggacaaag
     8701 aggaatagca ttaataactg gttgtgtaag agaatatcaa atagttggtg ataatttaga
     8761 tacaatagca ttaacactat tcaggtcatt tggatatatg ggaagagaaa atttacttta
     8821 tagaccagga agagcttctg gagaaaaaat aatagcaaca ccagatgcac aattacttaa
     8881 agagttaaac tttgactttg gtctttacat atataacaat aaatttgatg aagcaaatgt
     8941 agctaataca gctaagagat tcttaacacc tatacaaata tatgaatatg ctgatttttt
     9001 aaatggtaga cttatatttg cattcaatga cgaaaaacaa atatatgaaa atgaatatag
     9061 tttgatgaat gtaaataatt caaatttcac agtaagtgca ataaagaaag aagaaaaagg
     9121 agatggaatt gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta agggaagtct
     9181 taacatcaat aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa tctatgataa
     9241 tactagcaag cttaaagtaa atactaaaac tgtagaggta aattctttat cacattgtaa
     9301 agtacaaaca atagtaatta aataatataa aatagcttat acaagatttc aaaataaatt
     9361 gctagtacta tgttaatagt aaacccagat attatgctat ttataagctc tgtaacacta
     9421 aataacattt tgtaaaataa aaaggtgaac cttgtatatt aggattcacc ttttttaaat
     9481 aaatcaacat tctgttttaa aataacattt ataagctgtt ttgaaataat gcttacaaat
     9541 tattttaaaa caatacctat aaatttttat ttaaagaata cacataaata tttccagact
     9601 cttttattct atagacttta taattattaa ataagtctaa tctaattttt gttaaaagta
     9661 attgtaactt taaataaatc accatccaca cttatattca tcttaccacc ctgtaattct
     9721 ataaaacttt ttgctatcgc taaaccaaga ccagaacctt ctgtgttacg tgatttatct
     9781 cctcttacaa atctctccat tatatcatct acactaaaat caatctcaga tgcagacata
     9841 tttttaagca taatatttac tttattatct gtttctaaaa tatcaatata cactcttgaa
     9901 tttggcattg catatttgct tatatttatt ataagatttt caaatactct aaatgtacgc
     9961 tgactatcta atggtaatat tattttttca ttagaaaagt tattctttac tattagagat
    10021 gaatctgcaa gtttgtcatc taattctaaa agtgtctgct tcattagaga tacaatatcc
    10081 acatttacta tattcaaata aacatctcca ctatttgcct tgcttacttc aaacaaatct
    10141 tctattagat gttgcaatct ttgtgacttt ctgtctaatg tatcaatata caacttacga
    10201 ttttcatctg ttatactttc atcttttaat aaatctatat aagtgattat agatgttaga
    10261 ggtgttttta agtcatgtga tacatttgat ataagctctg ttttcatctt ttgactctta
    10321 acctcttcgt caacagcttt tttaaatcca gtttgaatat ttcctaattc gtcttttata
    10381 ggattaaaaa atcctaaatc tttttccata tttacatcta agtttccatc tgaaattttc
    10441 tttgtaacat ctaaaagttg attatattca tatattgtct ttttagagta atctgacagt
    10501 atcttaaata atactactga atatattaat gcgagtatta ttccaaagaa ccacatacta
    10561 cacattataa ctataatcaa taaatttatt cccaaaattg taactatagt tttatttggc
    10621 ttttctttta gatttattgt tctaatttga ttaataactg tttttcctgc tttttttagc
    10681 catacaaaaa acattcctgt catagtatgt tttgtaaaat actctcttat tccaacattt
    10741 aaaatgtgtt ttaaaagtat tataaataca aatattattg agaatattac aaaccaataa
    10801 gttaaattca ttaaacttac caatatgcta gccattttag cacttatgtc attgtttata
    10861 aggtactcta cataattccc actcaaggta ttacttatta taacagtagg atataaatct
    10921 acaagcccct gtataatagc aagagcaata aagagtatct ctaacggtat tcttgataat
    10981 gtatcaaatc ctagcaactc tttagatttt ctatatggga atgctaaagc tacaataaca
    11041 actatagcta atataatttt taaaatcatt ccagaaatct tataataaga attattttct
    11101 acattgctta tatatgaaat tataccatca tcaaaataag tatttccata accatactct
    11161 acactatcac ctttacttat tgctttaggt acagcatata ctaatgttat attttttatt
    11221 ggagataact tatatgaaat tgaatttcct cctaaacttg tatccattaa atcttctctt
    11281 gctttagata acaacatttg attaataact ttttgattta ctccataaga atctacaata
    11341 tttacttttc catctttatc aaaattcatt acaacgtaga atctatagtt ttcttttata
    11401 tctgtaatct gcttttgtct ttcctcatca ttcatttctg tattttttga ctgattttca
    11461 tcagaatttt cttttgaagc atcattcttt gttgtatcaa gtactcctaa ttctttatta
    11521 tcattaaaaa attcaaaatt gctttcttta tcaactaaat agtactttaa attctttcct
    11581 gacgctttaa tgaaatgctc ccaattttca aagttttcat taaaatctgc attaatacta
    11641 tcatctgtat agtaagtttc tgaaccatca taagtatctt tttctttact tacatttttt
    11701 atatagttca tagtttcttc attgataaat agctttgatg gttgtatcat cttaccttct
    11761 tgttcttcct tcatattatg atataaaaca taacttcctt tatatatact atctacaaag
    11821 ctttcctgtt caaatggatt ctctgctcca cctttagcac ttgcttgtat agtagaatat
    11881 tgactaatca tacctaatga tgctgataac ataagtataa aaactataat agtaactata
    11941 tatttatctt taaatttttt caattttgta tccaacaccc cacaccacct ttaaatattt
    12001 aggatttttt gggtcaattt ctatcttttc ccttatattc ctcacatgta ccataactgt
    12061 atctgtattt acagcattct cattccaaac tctttcatat atttcatcag cagaaaaaac
    12121 tcttcctgca tttctcataa gtaaacttag tattttaaat tctataggag ttgtttttat
    12181 atactttcca tcaatactaa cttcttttgt attttcattt aattctaatc cgcctattgc
    12241 aaacacacta tcgtcacttt ctaccttttg ctctttattt ccaaccatgt ttagatactt
    12301 agtgtacctt ctaagttgtg aattaactct agccaatagt tctaatggct taaaaggttt
    12361 tgttacataa tcgtctgctc ctatattaag ccccattatt ttgtcaactt cctctgattt
    12421 tgcagatagc attattactg gaaaattata gttttctcta agcttcattg taagtgttat
    12481 accatccatt ttaggcatca ttatatcaac tatagctaaa tgtatttctt ggttttctat
    12541 aaccttaaga ccttctaatc cattgtatcc cttaaaaaca ttatatcctt gatttaatag
    12601 atatatttct atagcatctg ctatctcctt ttcatcttct accaccaaaa tattatatga
    12661 attcattcta atctccttta tattaattta taaattttct ttacatatat gattatacaa
    12721 tatgatagaa gtttttccat ttatatatat atttatatct tttgttctgg ttatttgtat
    12781 ttaatgtcca tcttagaaac ctccttctag tttaaattta at
//
LOCUS       DAMJYC010000109        12540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJYC010000109 DAMJYC010000000
VERSION     DAMJYC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.113"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            34..1464
                     /locus_tag="PC472_003292"
     CDS             34..1464
                     /locus_tag="PC472_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2728595.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1664..2248
                     /locus_tag="PC472_003293"
     CDS             1664..2248
                     /locus_tag="PC472_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2728596.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2352..3131)
                     /locus_tag="PC472_003294"
     CDS             complement(2352..3131)
                     /locus_tag="PC472_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2728597.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3188..4444)
                     /locus_tag="PC472_003295"
     CDS             complement(3188..4444)
                     /locus_tag="PC472_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2728598.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAVEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4702..5601
                     /locus_tag="PC472_003296"
     CDS             4702..5601
                     /locus_tag="PC472_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2728599.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEKAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5625..7073
                     /gene="ddlR"
                     /locus_tag="PC472_003297"
     CDS             5625..7073
                     /gene="ddlR"
                     /locus_tag="PC472_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2728600.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7132..7938
                     /locus_tag="PC472_003298"
     CDS             7132..7938
                     /locus_tag="PC472_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2728601.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEMLFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSKVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7964..9883
                     /locus_tag="PC472_003299"
     CDS             7964..9883
                     /locus_tag="PC472_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2728602.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10125..10472)
                     /locus_tag="PC472_003300"
     CDS             complement(10125..10472)
                     /locus_tag="PC472_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2728603.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10459..12387)
                     /locus_tag="PC472_003301"
     CDS             complement(10459..12387)
                     /locus_tag="PC472_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2728604.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         4757 a         1829 c         1724 g         4230 t
ORIGIN      
        1 atatcattaa aaatttagga ggaaaatata aaaatgaatt tagatttgga attttatcaa
       61 aaaatattag aagcttccca tgatgaaata tgtgtttctg atgataaagg aataataata
      121 tattgtaata aagcatttga agaaaattat ggtctaaaaa aagaagatat tttgggaaaa
      181 aatgtttctt ttttagaaga ctctggatat tcaacaaaaa gccccatacc aattgtatta
      241 aaaacaaaga ctaaattttc actggaacaa gatactcaaa cagggaaaaa gcttatcata
      301 actgctaccc ctatatttga tgaaaatgga cacttagaat ttactgttga aaattgtaga
      361 gatataactg aacttaataa tataaaaaat aaacttgaag acacaaaaaa gcaagtaaaa
      421 aaatataaat ctgaagtaga aacactatac agaactgcac ttagaattga agatactgta
      481 attatggacg gaatagttat gagaccaatc ataaacacag taaatcatgt ttctaaaacg
      541 gatgtaagtg tacttttatt gggtgaatct ggaacaggaa aaagctcact tgctagatat
      601 atacaccaca acagcaatag atctaatggc ccatttataa caataaattg tgcaactata
      661 tctccacaac ttcttgaatc tgaacttttt ggatatactt caggtgcttt tacaggtgca
      721 agtactaaag gtaaagttgg gctcgtagaa ttagctaatg gaggaactct ttttttagat
      781 gaaataggag atataccaca aaatctacag gcaaaatttt tgcaattaat acaggataga
      841 acttttactc cagttgggag cttaaaaaat aaaaaggtcg atataagaat tatatctgct
      901 acaaatgttg atttagtttc aaaagtaaaa gaaaaaaagt ttagagaaga tttatactat
      961 agattaaatg ttatagaaat aaaattgcct cctcttagag aaagaaggga taatctagtg
     1021 gaaattataa aatattattt taataggtac tcaagtgact tcaatctaaa taaaactatt
     1081 tctaaagaag ctatggatgc tattgcaaat tatagattcc caggcaatat aagagaatta
     1141 caaaatataa ttcaaaaaat cttgcttact tgtacagata atcatataac aatagatgac
     1201 ttaccaaaca ttcttacaaa aaatatacat attaccaata atggaaataa aacacatata
     1261 tcccaaatta ataaggttat tatatctgac tcaaaatcta ctaattataa aaataaaaat
     1321 tttgacactt taattaaaga gtatgaaaaa aatataattt tggatgctta tgaaaaattt
     1381 ggtagttctt ataaagtagc caaacacctt gaaatctcac agtctaaagc caataggtta
     1441 attagaaagt atactaatac ttaattttaa atttgataaa taaaaactta acactattta
     1501 catattgtaa atttggccat ttactgttat tatgaatatg gttgaaatta tttaaataaa
     1561 tactgttgac ttagtttcat atatttacca ttaaactttt atcataaata tcatatcata
     1621 ataatatatt taataagtct aataaactat acgaggtgta aatatgttaa tgaataccca
     1681 ttttattatg gcaaaatcaa tacttgataa tatagatgaa aataaaacat tctttataag
     1741 cgaaaaaaat tttatatatg gcaatataaa gcctgatgct ttttctaaat ataaattaaa
     1801 aaaacattat ttagatgagt catttaatat gattgtttct aaaataaact atctatgcag
     1861 tcttaatata gatagtcttt ctaaatactt ttcagtaagt agacttagtc aggaactagg
     1921 agtcatatgt cattttttat gtgatttttt ctgtgtagct cacagtgaac gttgggagtt
     1981 taagcatagc ttaaataaac atgttactta tgaaagagaa cttggagcat ttgcaaaaga
     2041 tgttgattta tctaaaatta aatgtggtac tataaatagt agttttgaga agttttttac
     2101 taaactttat agtgattaca aaaagaaaac tgattatatg aatgatttaa aattttcagc
     2161 ttatacttgt aatagtgtca taaattatat acttgactct attttatcta atacaataaa
     2221 atcatatcat atagcaaatt gcggttaact taagaatttt taaacttata taaacttata
     2281 attgaatata attaaaatga ctgctctata gaatataatt ttatacttct ctaggacagt
     2341 cattttttat actaactatt tatctccatt ataacatcta tggcactttt tacactatct
     2401 actacataat ttgcactttg ctttacatct tcccttgcct tagacattgc aaaactatat
     2461 ttaaatcctt ctaacatgca aatatcattt ggagaatccc caatagtagc aacttcatct
     2521 ggacttacat ttaacgtttt tattacatgc tctatacttc ctctttttga cacactttta
     2581 ggaactatat ttaaagtata tgttccagaa aaatatattt ctaagacttc actaaactca
     2641 ctttttagat aaagttctat tctggataac tcttccaaat tttcagaaca tacgcctata
     2701 tttcctataa gattatcaaa actacttctt ttttcaaaat ctgggtcaac tataaagtca
     2761 ctagaataat gtctaaaagc agtggtatca ctacccacaa ttacatcacc cttatattta
     2821 aaataaagtt gtgcatcact tgactcaaat ctatcaatga ctttttttac taaattatcg
     2881 tctatcgttc ttttaaaaat tacattataa tctttatcat atataaatga accattttca
     2941 catacataat atccattcat atctaaacta tctttgataa tattatgtgc ttgtttaaaa
     3001 attctacctg tagcaacatt aaactgtact cccttttttt ctatttcttc aattgctact
     3061 atatcttctt ttgttatggt tccattttca tatagagttc catctaaatc acaaaatata
     3121 tgttttatca taaattaaaa gcctccaaaa caattttata ttttactaat actttaatca
     3181 aaataactta aagcttttct aattgtattc tcaattcatt aagtaaatct ctagtagctc
     3241 ttaatcttat ctttcttcta tccccattaa atatattttt ctttactatt gtttttccat
     3301 ttatatatat tgcagtataa accagtccca caggcttctc tttggttcct ccaccaggtc
     3361 cagctactcc agttgtagat atacctatat tagctttaag tcctttagca acgccctcag
     3421 ccatttctac agctgtttgc tcacttactg ctccaaactc ttccaaagtt tctcttttta
     3481 ctcctaatct tttcatttta gaatcattag aatatgttac acaaccttca agaaatacag
     3541 aggatatacc tggatagttt ataagagatg aagaaaccaa acctccagta caagattcag
     3601 ccactgctat tgttagattt ttttctacaa gtagcttaga aacagcatct tctaaagcag
     3661 tctccccttc agatatggaa atatcgcctt cagtatatac atatttacca actctacttt
     3721 ttattttttc ttctactggt tttattaatt taaaagcttc tctttcattt tcagcctttg
     3781 cagttattct tattgtaact tctaattctt ttgcatataa agcaactgtt ggattagtct
     3841 gctcattaat tatatcgaga atttctaatt ctagatttga ctcacctatt ccatataatc
     3901 ttaaagtctt agatactagc atttcatttg tgaattgttg taagtaaggt ttgacactct
     3961 cattaaacat agctttcatt tctcttggag gtccaggcaa tactattata aatttaccat
     4021 ctttttttaa aattgcccct ggagctgtac cattattatt ttttagtact atagcatctt
     4081 ttggaaagta cacttgtttc atattatttt cggccatttt tttccctgtt ttaacaaaga
     4141 aagattccaa aacctctagt gaaggtttat gtagttctaa tttctgacca aagtattctg
     4201 cagcagtttc ttttgtcata tcatcaccag ttggcccaag accacctgta gtaattacaa
     4261 aatcacttct ctttaagctt tcatcaaaac attctaaaag tctttgtttg ttatctccaa
     4321 cagtactctg atggtatact tctattccca atgatgcaag ttctttagct aaaaattgag
     4381 aatttgtatt tactatatct cccaataata tttctgttcc tacacttatt atttcagctt
     4441 tcaaaattta tcactccttg atttatagcc attaagacta gtcttaagtt cataacaata
     4501 attttagcaa aaataataac ataaataaag ttaaaaaatc atttaaacat atctttaaac
     4561 tagattatta ttctaaaacc acacataata taaacttcgt atatagtggt actttcatcc
     4621 tcaactgtta attcatataa ttaagtatgt aaataaaaag tttactttaa aataataaaa
     4681 attaaaatag gagtgtataa tatgaaaata gcagttataa tgggtggaat atcatcagag
     4741 agagaagttt ctttaaattc aggaaaagag atatataata accttgataa aaataaatat
     4801 gaagttgtaa aagtaataat agatgataaa aaagatatat ttacaaagat accagaagat
     4861 atagattttg caatacttgc tttacatgga aaatttggtg aagatggatg tatacaatca
     4921 atacttgaga ctatggatat accatactct ggatgtggac ctctttgcag tgggatgtgt
     4981 atggataaaa atataactaa aaaaatgctt agagattcaa acctacctac tgctccatgg
     5041 gttttagtaa aatcagttga tgaaatagac tatgatgaaa tagataatat aggttatcca
     5101 gtatttataa aaccaaactc aggaggttca agtgtagcca cattctttat tcattctaag
     5161 gatgaagttg aaaaagcagt tagaaaaggt ctggaagttg atgaatttgt aatgattgaa
     5221 aaatatatac caggtggaga atatacttca ttcatattaa atggagaagt gttccctaca
     5281 atatctataa aatctgattc aggattcttt gattatgaag ctaaatactc agttgaaaaa
     5341 ggtgcaaagg aagaagttgt ttacttagat gaagaacttc aaaaaagagt aaacgaaatt
     5401 tctgagactt gttggaaaat ttttaattgc aaagcatatg taagagttga tatgattata
     5461 agtgaaggta ttccatatgt tcttgaacta aatactcttc ctggaatgac tcaaactagt
     5521 ttaattccta gaagtgctgc tgctagaggt attaagtaca gtgaactatt agataaactt
     5581 atagaatact ctttaaatta gatgaaataa tttgaggtgg taatttgata ttttcaaatc
     5641 taaaattaaa tgataatgaa ccaatttata tacaacttaa aaattatata agtgatatga
     5701 tatcaaaagg actaattcca gacaatagta agctcccttc tacaagggag cttagtcagc
     5761 ttttacaagt tagccgaaat tctgtagttt taacttatga agaattaaaa tcagaaggat
     5821 taatctactc catttcagga aaaggtacat ttgttaaatc aaagaataaa tcttcaaata
     5881 ctacatggtc attaaactgg gattgtttag agaatacata ttctaaaaaa gccaatgaat
     5941 tggacattat aaaaagtgaa attccttgga gctctgacct gatttccttt aaaagtattt
     6001 ctccagatgg agatttattc gacatggaag aattaaaaaa atccttttta aatagaattt
     6061 cactagaagg acataaatta ctcaactatg gatatgcaca aggttataag ccccttattg
     6121 actatttact agaatatatg acaaacaagg gtgctgatat aagtaataaa gatatactaa
     6181 taacaaatgg attcacagaa ggctttgata ttattatgtc atcttttacc caagaaaaag
     6241 actacataat atgtgaaaat cctactcata atactgctat aaaaattatg aaatctcata
     6301 acctcaatat cttaggtgta gatataaatg aagatggttt ggacttcaat atgctagaag
     6361 acaatttagt taagtataaa aataaaataa agtttgcata cattacaccc tcttatcaca
     6421 atcctacagg aattgtaatg actcctgaaa ataggtacaa attctataat cttatgaaaa
     6481 aatacaatat agcaattata gaagatggat ttaatgaaga attactctac aatagttctc
     6541 atatatttcc tatctgttcc ctagataata tgaataatgg ggtcgtatat attggtagtt
     6601 tttcaaaaat tttatttcct ggaatgagaa ttggatggat atttgcagat aaaaaagtaa
     6661 tcaataaact agaaagtgta aaaaggtgca gaaacataca tgtatccttt ttagaccaag
     6721 gaattttatt tgattattta agtaatggag gttttgaaaa atacattaaa aaaatacgta
     6781 aattctatgg agacaaattt aactttgcat ataagtgtat aaaaaaatat ataaaaacag
     6841 aatatatctt gggtgatggt ggtttgcata tatttataaa actaaaaaat atagatacta
     6901 gagttttatt agaaaaatgt tatgaaaaaa atgttatatt tatgcctgga gatttatttt
     6961 ttacagataa ctctggtttt gatactttaa gactcggatt ttctaggtta tcttttgaag
     7021 acattgaaat tggaataaaa ataattggag aaactataaa tgaaatgttc taggttaact
     7081 aaatattgtt agcctagttt atttttatga ggtataatat atatagttta tatgaatcta
     7141 atactggggg ataatatgag taaaaagtca aaacgaagaa aaattaataa attaaaatta
     7201 acgattgtaa taactgtatt atttcttgct ttattatcta tatatgagat gctgttctct
     7261 aatcccaaca aaaaaggtaa tataaagagt caagaaacta tgcaaaataa aaataataca
     7321 acagatgatt ctaaaaataa tcaaagctct accaaaaagc aggaagataa taatatcaaa
     7381 cctacttttt catattcttc aataccagat aatataaaaa ataaaatgat tggtaaatct
     7441 atgcctactg atgaacctat aagctttgat agtctatctt atttaaaact tacatattat
     7501 ggatttgatg aaaaaactca tcaaggagaa atgatagtaa acagtgaact agctccagaa
     7561 gtagtggata tttttaagga attatacgaa aaaaaatatc caattgaaaa aattaagtta
     7621 atagatgact atgatgctgt agatgaaaaa tctatgtctg ataataatac ctcatcattt
     7681 tgttatagaa ctatagctgg tacaaatgta gtttcaaatc atggtaaagg acgtgctata
     7741 gatataaacc cattacagaa tcctcaagtt tctggaaatg atgttacacc taaagtaagt
     7801 aaagtttatg ctgataggtc tagcacaaaa tttggaatga taaaaaaagg tgatgattgt
     7861 tataatgctt ttgtaagcag aggttggagt tggggtggct attggaaaaa cccagattat
     7921 caacattttg aaaaataata gattagaaat aaaggtgaat tatatgaatt taatgacatt
     7981 ggaaaatata agtaaaagct attctgaaaa aaaattacta gaaaatatat ctcttggaat
     8041 aaatgaaggg gaaaaaatag gacttattgg tgtaaatggt actggtaaat ctaccttact
     8101 caaaattatt gcaggagttg aagaaagtga gactggtaat ataacaaaaa ctaatggtat
     8161 taggatagaa tatctacctc aaaatccaac ttacaatgaa aattcaactg tacttgaaca
     8221 agtatttaat ggaacttctg aagaattaaa gctacttggt gaatatcaag aaatcctaga
     8281 aaaacttaat tcaaacttta cagaggaatt aaataaaaaa ttattatcag tacacgaaaa
     8341 aatagatgcc ttaaatcttt gggatttaga aagtgaagct aagtcagtac ttacaaagct
     8401 tggtataaat gactttaatc aaaaaatagc agaactttct ggtgggcaaa gaaagagagt
     8461 ctctctggca tcagccctta ttacaccttg tgagtttttg atactagatg agcctacaaa
     8521 ccatctagac aatgatacta tagattggct tgaggaatat cttaattcca gaaaaggttc
     8581 attgcttatg ataactcatg atagatactt tttagaccgt gttacaaata gaattattga
     8641 actggacaag ggtagattat tcagttatga tgggaattat tcaatatttt tggaaaagaa
     8701 aatggaaaga ctttcgctag aagcaagtat tgaagataaa agacaaaatt taataagaac
     8761 agagcttgcg tgggtaagac gaggagctca ggctcgtagc actaaacaaa aagctcgttt
     8821 acaaagattt gatgaactta taaatagaga ttcttttaaa cctgatgaaa atctcgatat
     8881 atctgttgca tcaagtagac ttggaaataa aataatagaa atacacaata tatctaaatc
     8941 atttgaagaa aatactgtta tagataattt agaatacact ttaactcgta cagatagaat
     9001 tggtataata ggcaaaaatg gtatgggtaa atctaccctt ataaatatac ttaatggaga
     9061 aatagagcct gatagtggat atatttcaat tggagaaacg gttaaaatag gttgtttttc
     9121 tcaagacgac tctcatatga atattgatat gagggctata gactatgtaa aagaagctag
     9181 tgactatata gaaacttcag atggtactag gattacagct tctcaaatgt gtgaaagatt
     9241 cctttttaat ggtactatgc aatatacttt gataggtaaa ttatctggtg gtgaaagaag
     9301 aagactacat cttcttaaaa tccttatgac agctcctaat gtgcttcttt tagatgaacc
     9361 tacaaatgat ttagatattg aaacgcttaa aatattagaa gaatacctag acgaatttaa
     9421 aggtgtagta gttacagtat cacatgatag atatttctta gatagaattt gtaataaaat
     9481 atttgcatat gaaggtaatg gaaaaattca tatctataca ggtaattaca gtgattatct
     9541 tatttataga gagattcaag gaattgaatt tgaagaatct aaaaaggaat cagttaaaaa
     9601 agatgactct actaccctaa aaaaagaaaa acctaaaaat gataaaaagc ttaagttctc
     9661 atataatgaa caaagggagt ttgaaaatat tgactctgat atagaaaaac tcgaatctaa
     9721 aattgcaaca cttgatgaaa gtactgcaaa attttcaact gattttacta aacttcaaga
     9781 aattttagat gagaaatcta aactagaaaa agaacttgaa tacaaatatg aaagatggga
     9841 atatttaaat aatctagctg atgaaatagc taataataag tgatattaaa ataatcttaa
     9901 gtttaataag ttttgcttta aatctgtact ttataaacat tttaaaggaa gtgtctcaaa
     9961 atgaactttt tagttttatg agacgctctt ttttatgaga aatcaaatat gttttcatca
    10021 tttcaacaat acaaaagagg cttatctcta ttttgagaca gcctctttca taattacctt
    10081 ctaatttata tgctctaata tatctataat aattcttatt atatctatat atccagtctt
    10141 tcaatatagt gacaattaac ttcaatatga tttaaaaata ctaacaacct ttcataaagt
    10201 ggatgagatt cttctccata tttagcttct aggttcttgc ctatttctct aacagtcctt
    10261 tgaccatcaa tttgtaaaaa aacaaaactg gaatattcat ctagttctat ttttttgtat
    10321 ttaggtattc tagcttttaa ctttctaaaa aacttttgaa ttttatggtc ctgtttttca
    10381 agtatagtaa caacactatt ttcagctact tcatattcaa gtccatcagt aattttaaat
    10441 acaatatcta aaacatcatt actcttgctc attatttttt actttagata aaacaatagg
    10501 taatgctgta agcacaacta aaactactaa taaagcaatt gccattgaat ttgttgcagc
    10561 aaatccactt ggtacctttg gagttacaac tccagttact tgtaatatta tacctattaa
    10621 accaatgata gaaccaccag caacaagacc agaagataaa cttataccat tagaaacttt
    10681 aacttctttt tctttttcag ttttagatac taattcaaca aataaacgaa ttaatgctcc
    10741 aactaatatt atagaagttg ttgcaattgg taaatagaat cctatagcta tagtcattat
    10801 tggtaaattt aatgcatata gaactattgc cataaatact ccaacaataa tcataaccca
    10861 aggtaaacta cctgacataa taccagaagt taatgttgac attaagtttg cttgtggtaa
    10921 tgcaaattgt acattatcac cagttgctct aagttggtca gaaagtaata aaattactcc
    10981 tacaacaact ataacaccaa ctatacttgc aattgtaaag taacgttgca tttcgttttt
    11041 gctaccacca ataatataag ttactttttg agattgagta taacctccag ctatagcaat
    11101 agcaacaact ataaatgaac caaataaaag taatgattta ttagcttcta aatcagtcca
    11161 tcccattata acaaacacta atgttacaat aactagagaa gctatagtca taccagaaac
    11221 tggaagattt gatgttccga ttgttcctgt taaacgacca gctactatga caaataataa
    11281 agataataaa agagatatga tagctccaat aattgccata acaatgttac cagaaattaa
    11341 gaatgctgct aaaaatccaa taactactcc acctaataaa agtatcattt gtatagatga
    11401 accttcttct ccctctccag cacttgattt agctttaagt gtttctttta tagaagcgat
    11461 tatagttggt ataagtttta ttgcaccaat aatacctcca caaagcatca taccagcacc
    11521 tatatatttt acataactac tagatattgc tccaacatcc atttggttaa gaggcattgc
    11581 aggattattc cataccatag ctccatcttt agccatatct gtaaagtatc ctattaaagg
    11641 tgctatacca aagtttgata aaatacttcc tgcaaacatt gttaaagaaa cttcaagccc
    11701 tactataaat ccaattccta aaagtagagg atttacttca atatcaaatt tccatttgta
    11761 gaatttgctt cctacaagac tcattacatt gtttgctaca tttaagaatg aaccagttaa
    11821 tacagtaata aatccactta ttacaaatcc aattcccatg tacttaattg aatctccacc
    11881 agcctctgaa gcaacaagtg tctcagatat agccattgat tctgggtaca taagtttacc
    11941 atgttcttca actattagat agttgtgaac tatagcagca attcctatac caaataaaac
    12001 tccaccaact ccaacagcta agccttcaaa gaatgtaatt tggcttccaa ttaatataac
    12061 tgctggtaat acgaaaataa atccacttgc aacagattct ccaccacttg acataccttg
    12121 gattaagttt ttaccaagaa taccctttga acgagcaaat gctccaacaa aagcagaacc
    12181 tattatagct cctggaatac ctgcggcaac tgtaagaccg gctttcattc cagagtaagt
    12241 tgtagatgcg gcaaaaatgg cagctaaaat aataccgata atcagtacag ctacatttcc
    12301 accggttctg gacttatctg tgatatatgg aacgtaatct tttccactaa cctcaccata
    12361 agcaccctta ggtaattttt tgttcataaa attttcaccc cttttcaatt tgtatatatt
    12421 ataacatata tacatataaa acttaacttt ttattatttt acaaatagcg agtcaaaact
    12481 actaaaattt aaccctatat tattaatttc tacttttaga tatttttatg ctaattttct
//
LOCUS       DAMJYC010000110        12484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJYC010000110 DAMJYC010000000
VERSION     DAMJYC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.114"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            321..1256
                     /locus_tag="PC472_003302"
     CDS             321..1256
                     /locus_tag="PC472_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2728605.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1348..3219)
                     /locus_tag="PC472_003303"
     CDS             complement(1348..3219)
                     /locus_tag="PC472_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2728606.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3409..4335
                     /locus_tag="PC472_003304"
     CDS             3409..4335
                     /locus_tag="PC472_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2728607.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4474..4620
                     /locus_tag="PC472_003305"
     CDS             4474..4620
                     /locus_tag="PC472_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728608.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4725..5249)
                     /locus_tag="PC472_003306"
     CDS             complement(4725..5249)
                     /locus_tag="PC472_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728609.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5302..5526)
                     /locus_tag="PC472_003307"
     CDS             complement(5302..5526)
                     /locus_tag="PC472_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728610.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5723..6847
                     /locus_tag="PC472_003308"
     CDS             5723..6847
                     /locus_tag="PC472_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2728611.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGILDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7321..8145)
                     /locus_tag="PC472_003309"
     CDS             complement(7321..8145)
                     /locus_tag="PC472_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728612.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8493..9548)
                     /gene="ispG"
                     /locus_tag="PC472_003310"
                     /gene_synonym="gcpE"
     CDS             complement(8493..9548)
                     /gene="ispG"
                     /locus_tag="PC472_003310"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2728613.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9731..10735)
                     /gene="rseP"
                     /locus_tag="PC472_003311"
     CDS             complement(9731..10735)
                     /gene="rseP"
                     /locus_tag="PC472_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2728614.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10748..11902)
                     /locus_tag="PC472_003312"
     CDS             complement(10748..11902)
                     /locus_tag="PC472_003312"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2728615.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12066..12269)
                     /locus_tag="PC472_003313"
     CDS             complement(12066..12269)
                     /locus_tag="PC472_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728616.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         4168 a         2098 c         1296 g         4922 t
ORIGIN      
        1 taacttatat actttatgtg taaattatgg taaaaatatt atttaatcaa atcaaaactt
       61 gatttatata cctggaaaag gtattatatt aaagagtgct taaaatacgc gtttcataaa
      121 tagctatata taaaataata aataaggaga gtgatgggca atggatacta gtccgttgcg
      181 gagagaattt tttacatttt catcacttaa taatatctaa aatcaaaact aatacatata
      241 ttcagttata taaattagtt aattaataga tactattaaa tgatgagatt tatataaaac
      301 tgaataaagg atgtgttgcc atgataagat attacaaaac tatagattca aaattagaga
      361 aacttagttt ttttgaagat ggctgttgga taaatcttgt tgaacctaat catagtgaaa
      421 tcaacgaaat ttctaattta ttaaatatag atgtagaaag catagaatct gcactagatg
      481 aagaagaacg ctcaagaata gatgttgaag acaatcatac tcttatactt atagatatac
      541 cagtagacga aagtgactct aattcttctc attatactac aataccctta ggtattatac
      601 taactgaaga agctattgta acagtttgtg atgcacaaac taagatttta aatgacttta
      661 tagttggtca tattaaagat ttttttacgt ttaaaaagac tcgctttttg cttcaaattc
      721 ttcacaaaaa tgcagcttat tacttgcatt acttaagaaa aataaataaa atgacaatca
      781 taatagagcg agaaatttac aaatcaatga aaaacaaaga attggttcaa ttattggaat
      841 tagaaaaatc tttggtatac ttctctacct cactaaaatc taatgaactt gttttaaaca
      901 aaatggttag aactgctggt ataaagaagt atccagatga tgaagaccta ttagaagatg
      961 ttatagttga aaatagacag gctcttgata tggctaaaat atatggagat atattgagta
     1021 gaattatgga tgctttcagt gcaattataa gtaataatca gaataatgtt atgcaaattt
     1081 taacagtagt tacactaatt ttctcaatac ctacaataat atcaggattc tttggtatga
     1141 atgtcatcaa catgccattt tctaataacc caaacggatt ttggataata cttcttatta
     1201 cctctataat atgtatagtg attacatttt tcatgtcaag aaataagcta ttataaaata
     1261 ttaataaaaa agccattaga attaattgat ttacatttat cctaatggct tttatatatt
     1321 taaatctata taatcttata atacttttta actatcatgt tcttcatcta taatcatttc
     1381 agtttcaagc ccttctagca ttgaatctaa actttctttt atatttagta tataaattgg
     1441 tacatactct tcttcatcta ttacctcttc ttgcaactta aatggaggaa ttaatacttc
     1501 atcaaagtct atatttaaat ttggaaagcc ttctactatc tcttcacaat tatcgtactc
     1561 ttcatttgct aaagaaatgt acatatctag gctcttttta taataaagac ctgcaagcat
     1621 atactcacac ttcttacttc catatttaat accttcttta taatatttct cagcttctgc
     1681 gtcatcattt aattttaaat aaatttgacc taaattatat attgattcaa cacaaccttg
     1741 tgctctagcc ttttcatacc aaaatttaga atttacataa tctttataga acatatcata
     1801 aattaatcct accatatgtt gagcatacac attattttca ttggcaggtg tatcaaacat
     1861 cattttagac tcttccaatt taccttctct aaaataaatt ctacctagat gtatacgagc
     1921 attcatatgg tttttgctac ttgccatttc aaaatacttt ctagcatttt ttgtatttcc
     1981 taagctctca tatatacacc ccaatctata actagacctc gtatctccat tttcagatgc
     2041 tttcttatac caacttattg cttcttcaat attatttaaa tcttcataga tatttcctaa
     2101 tttgatttga caagatacat tatttggaat cctcgaataa tacgaaatcg ctttttctat
     2161 atcttgattc tgataatata aatcccctag attttcaagg gaacttttgc atccaacagc
     2221 tatagcatcc tcataatatt taatagcatt ttcataatct ttttcttcaa aatatatacc
     2281 tgccagatta ttttttgctt ctgttatatc atcttcagca gccattttat aataagtttt
     2341 tgccttctct atctcatttt ctctattata taaattagca agtctatact tagaaagtgt
     2401 atgatttttt tctattgctt tctcataata ataaattgca tctcccagct cttcttttct
     2461 atcatataca tatgcaagcc tatactcaga cttcacatca tctctatcag cactcttttt
     2521 ataatacttc tctgcatctt ctaaattatc atttatttcc ataatcatac ccaagttaaa
     2581 gcaacctttc atatgacctg acttgtacaa ctgtttatat aaacttactg ttgatttatt
     2641 atctttcttt tttccattta atattgctaa attatatttt gcatcatcat ctaaaagcac
     2701 agaacctttt ttatagtatt catatgcttc atcatctttc tccaaaaaag tatataatac
     2761 ccctaaatta aaacaagctt tacctaaatt cttgtctgct gctgttttta accatttttc
     2821 acaccttaca aaatctttca ttttaaaatg gcatacacct aaattatttt gtgcttgtaa
     2881 ataacctaaa tttgctatct tttcatacca atatatagca tattcatagc catctaactt
     2941 atagtacata ttacctaaat caaaagcagc atcttcactt ccacttttat atgccattga
     3001 ataccacttt tctgcttctt taaattgttt tttactttca tatatatttc ctaaagtata
     3061 ttgagatttt acgtaattat ccaatgcagc actcttatac aactctattg cgctttccat
     3121 cttacccatc ttttcaagga gggcaccctt agtatgtacg actttcgggt cattagatat
     3181 attttttatt ttttttatag aaaattttgt atttttcatt attcgctacc tacccatttt
     3241 ataaaatata ttctctcaaa cattattata cattataatc tacaattaat gctataatat
     3301 tgaattttta ccttttgtca ctatattgta acattaaatt ttaaatttta taatcattag
     3361 attttattat gtaattggta tataatgtat atataggggg gacattttat gaatttcagc
     3421 aatattttta tccaagttgc agttttattt ataattattt tagttggata ttttgtcaga
     3481 aaattcaact tattagatga ccactgcaca tctaagcttt caactttgac aatgacagtt
     3541 tttttaccat caatgataat tagctccatg caaattaact ttgatagtaa aatgatacaa
     3601 aaaatactat tgttattatt tacatctttg attatgtata tagtttcaat tataattgca
     3661 tttttattga aatacatttt aaaatgtaat gataaaaaag atttgggaat ataccagtat
     3721 atagttgttt tttcaaatgt tgcttttatg ggttatcctg taattgaagc agttttaggt
     3781 catgaagcaa tattctatac tgcaatattc aatttgcctt ttaacttatt ttctttcact
     3841 ttaggaattt atcttttatc taaaggcagt acaagtagag gtttttcaat aaaatcactt
     3901 ataagccctg ctacaatagc tattgttatt ggactttttt tatttgtaac aggacttagg
     3961 ttaccccaat ttataaacga gccacttgag atgttgggaa gtataactac accaatatcc
     4021 atgattatca ttggaagctt acttgctaat tcttcagctc tcgattgttt tgtcaacaaa
     4081 aaattatata tagtcacttt tataagatta ttggtacttc ctgtgattgt gtattttatt
     4141 ctaaaaggat ggataaatga taagatgatt ttagctatac cagtagtaat ttcttcaatg
     4201 cctgctgctg caaatactgc tataatggct aatcaatatg actccaatat aacactagct
     4261 tcacaatgtg tgttttttac aacactattt tcagttatat ctataccttt tataagtatt
     4321 ttactcttat cttgatttat ttttttatgt attatataat aacatattca ataaattaca
     4381 aatataaaca ttttattgta gtatattgat ggaatttttt cctagatatt tgtcaataat
     4441 atctatatgt attatagaat aggaggctta aatatggata tggatttact ctttaaaata
     4501 gtatttgcta tagttgctat aggtgtttct gtaaaaatca ttaaaactgt tgctagttta
     4561 gcttttaaaa tagcgttgat attgctaata attttatttg tatatagaat gtttttgtaa
     4621 acatttaaaa ataagttttt taacacaata gagaagtaaa atagattatc tttaatgtaa
     4681 attttttatt aaagacactc tattttactt ctctattagc ttatttaaat taaattttta
     4741 ttatatcttc ccataatata aacatcttca aattttccat ctctgattac agcatatttt
     4801 ttagttccct ctatttcaaa acctagcttt ttatataaat ttaatccctt ttcattatcc
     4861 acaattactt ctaatcctac tctagttaac ataatccaat tatctgctat atctaaaagt
     4921 tgttccataa gcttcttacc tactcctctt ccctgataat tctctgcaac acttattcct
     4981 atttctgcgc tatgtctcaa tcgtggagaa gaatttacat gtaacccagc aaaacctatt
     5041 acatcttttt ctccattatt attagtttca gctacaaaca tataatcatt ttctcccaaa
     5101 gaagctaaca tagcttcagt tttatcgact ctgtcactca ttaatgccaa agtatttctc
     5161 ataacactgt atgttcttct aatttcatta aaagcttgtg catcttctct tcttataggt
     5221 cttattgtta tatctatagg cttactcata ttggattccc cctcaaacat aatctttatt
     5281 tttaattaaa attttacatc tttattttat actgttcaaa tctatgtatg cttctacctg
     5341 aggtacatat atcatattat cttttatatg tgtacttcca aattctttta attctgtacc
     5401 ttctttatcc ttagttattt tatatctaat ggattccata ccttcaacta cttcagcatg
     5461 aaaaaacata ccttcatatt gtttaaatct aaaattaaaa ttgtctttag taagttttat
     5521 tatcatattt ttatctactc cttatataat tgatattttt cttattatta gttgtatata
     5581 tactgcttat tcatattaat atattataac ttatcattta tttagtataa agttataaat
     5641 agatatattt atacttattt tacaatcttt cttcaattga tatataatat gtttatataa
     5701 actaatgtat gtgaggtgaa atatgagcaa aaaaacaaaa tatttttctt taattctctt
     5761 tcctattgct ttgcttttaa attttatagc cagtaaagtt ccaaatattg ttgaaaaata
     5821 ttactcccaa ttcatagata aaataattgt acaaatattg agtaaagttt ctggaatttt
     5881 cccattttct ctctatgaga ttacaatgta cataatagtt atatctatct tcttattttt
     5941 atgttgtact ttatctacaa tttttaataa gaaaaaaaaa ttaaacatat atttaaaaaa
     6001 ttccatttta aatatattat ccattgtatc aatagtctac tttctttttg tagtgctttg
     6061 gggaataaac tataatagga ttccactaga aaccacactt ataaataact ataatttaaa
     6121 aaacaatacc tctatccaat caaaacaata tagtgttaaa gagcttacta aattatataa
     6181 attccttgta attaaagcaa atgaaactag aaaactcaca ttacaagata aaaatggtat
     6241 tgtaaaatct aatacagatt ataatggtgt cataaataga gctcaactag gatacgataa
     6301 tatcttggat atattaccta gtgttagtgg tagttattct aagcctaagt atattatttc
     6361 atcaaatcta atgtgctata caggaataac tggtatatac tttcctttta caggtgaggc
     6421 aaatgtaaat atagcaatac ctgacttata tattccttgt acagttgaac atgaaatggc
     6481 acatcaaagg ggatttgcta gtgaagatga ggctaatttt atagcgtatc ttacttctat
     6541 aaaacacccc aatattgatt ttaactattc tggatatatt ttagctttaa attatactgc
     6601 ttctgcttta agtaaggttg attataatgc atatgttgat atttctgctg gcattttaga
     6661 ttctgtccgt agagatttaa aaaatgaaag tgaattttgg caaaaatatg aaggtaaaat
     6721 taatgaaatt tctaatgagt ttaacaattc ttacttaaaa gctaatggcg ttactgaagg
     6781 tactcagagt tatggtaaaa tggttgattt attgctaact tattatgaat tatatccata
     6841 taactaaaca ctacaattca aaattttatt catatacagc tgatattaaa tcagctgttt
     6901 tctatttaaa taaattattt aacttcaagc aatcttatat aatttgatag tacttatact
     6961 atctgtataa catatcaatt ctaactattt tagttaaaac atttctatta tcttttttaa
     7021 taaatgtttt aactaaaatt tttaaaacat aaaacttcca cattatttca ctatctatat
     7081 aaaaatctaa aatttcacat ataaaattac ataactaaaa aatcataaat ttatagaaaa
     7141 catatattca aaataaaaaa gcatgtatca aaacttgaca catgcttata atcttatgta
     7201 tatatatcat tttgtcacaa taaaaattta cttgtaacac tgatattttt aaacaaaata
     7261 gtccttattt aattaaatta acattaatta aattcgtact cattttgcta tcattaaaca
     7321 ttacttatag taattttcta atacactatt tgaaacttcc ttcccttgaa aacttttcaa
     7381 ccaattctca tatgctgagt ttttccatac actaccatca ttaaatgtta cttgttttac
     7441 acaagatata atgtacttta caccatgctc tccagttgat tgtttccatt tatcaaacaa
     7501 tagaaaggca tcattttgat tcttacctgg tgctattgca ttttgctttg catattcttt
     7561 ctccatttgt ttccattctt tcaaccaatt ttctacccat gccttgcctt gttgtttttc
     7621 aaacatactt attatatcct gtggtggtga ttgttgcatt tttctataag tatgtgaata
     7681 tgattttgga gacacaggag atatgcctgt aacaatacca tagtctacac cagctgggct
     7741 aaatccaaca cttcctactt cgccatctgc tgccacattt tgagcatccc aatatagctc
     7801 aagtggtttt ccatttttat catatgctaa gcatgtaatt tcataatcca taattgcctt
     7861 ttcactgcta tttgttaagc ttataacatt gctaggccaa tgatatggag tatcatcata
     7921 ctgtattttg ctattattta taaataatgg aatattctca ttataaacaa gacctttact
     7981 actttgaata tctgatacct tctttgtgac agtacttacc gtatttgcta agtatacatt
     8041 tgttttttct gaattggtcg tactaagaga aaatgttgtt gtcgttccag taatcagcaa
     8101 agcaacaact aaaataggta agctagatat ttttttatat ctcacaattc ttcacctctt
     8161 tttctattaa cttataataa aacaatttac caaaacatta aaatctatgt taacataatt
     8221 taccaactgt tctagtaact atatcctatc atagttacta tcaagtttca atactattga
     8281 tggaaatttt cactatatta aatctttttt taattttata aatttacaat atgatttaac
     8341 tcatacatat atagtcaaat tagaatataa gtttcttaca tttaatgaag tttataaaat
     8401 aaaaaatgac tagataatta aatactgtaa ataaaaactt attacaaaag tatttaaaaa
     8461 tctagtcaat taatatatat atgaattgta tactataatt tttcaatctc ttctagcaac
     8521 tcatcaacta atttgttacc atcaatcatt ctaactattt cacccttttt aaataaaaga
     8581 ccttcacctt tacccccagc aatacctata tctgcctctc tggcttcacc aggtccattt
     8641 acagcacagc ccataattgc aacagtgata tttttttcca tactaccaat cttttcctca
     8701 acctcattta caacactaat taagtctata ttacatctac cacatgtagg gcatgatata
     8761 actttaatct tatcatttaa aagtcccaaa cttcttaaaa tctgtttacc aacaataacc
     8821 tcttcaacag ggtcgcctgt taaagatatt ctaacagtat ctccaattcc tttaagaaga
     8881 agtgctccta cccctataga agactttatt gttcctttgt gaacacttcc agattctgta
     8941 attccaatat gaagaggata atcaactttt tttgatatta attcatatgc atcaacagtt
     9001 ttataaatat cggatgattt tagagatata actatattat gaaaatctaa gtcttcaaga
     9061 atcttaatat gtcccattgc actttcaact aatgcttcag cagttgcaga gccatacttt
     9121 tttaaaagtt ctttctctaa agaacctcca ttaactccaa ttctaatttt taaatttctt
     9181 tccttgcatt tctcaactac catcttaact ctctctatac ttccaatatt accaggattt
     9241 attcttattc catccacacc ttgttctata gcttcaaggg ctagcttata atcgaaatgt
     9301 atatctgcaa taattggtat atttacacta gactttatct ttgctatatt tttagcagct
     9361 ttaatatcag gaacagcaac tctaactata tcacaaccag cttcctctaa tctctttatt
     9421 tgagctattg tagcatctgc atctcttgta tcagtatttg tcatagattg tatacttata
     9481 ggattttctc ctcctatttt tacagttcct acacttactt ctctcgtctt tcttctctcg
     9541 taactcatat tccacctcta tatattattt tatattgaat ctctttaaat attaaaatat
     9601 tataacctac atgtatatta taaattaaaa agctatctag ttaaataaga ataaatttca
     9661 tacataaaaa ttaatttatt cttatttaaa actacatagc ctattttctt aaaatctcta
     9721 aaaaactaaa tcattgaaat aatcttagta tatctttata tgtgataaaa agcataaata
     9781 acattaaagc tccaaaacca acaacattta tcatgccttc tttatttggg tcgagttttt
     9841 taccacctct aacagcttct agtaacagca taagtattct ccatccatca agtgctggta
     9901 ttggtaatag atttactata cctaaattta agcttatgac agctgctaaa tacactacat
     9961 ttatgatgcc tgtacgagca gcatctgata ccataccaat aacacctact ggtcctgcta
    10021 cagcattgcc aatccctcct ggtacacgtc ctgtaatcat ttgacctaga aatgttagca
    10081 tctgttttgt catatctaca gtagttttac atgcatttac tattgacccc aaaaagtctt
    10141 tttctctttg tggttgaatc cctatttcat actttccatt gttattttta ggtgttacat
    10201 ttacaacttt atcacttcca tctctagtta tactaagttt taattctcca ccagaagaag
    10261 tgtttattat atttgcaact tcatcccaag tcttaacaga attaccatta attttattta
    10321 tcttatctcc tacttgaagc cctacagcct gtgcaggagt atctggcatt atctttccta
    10381 atttagtcgt tggagaacct atatacaaaa atactggaac taaaagtatt gctgctaata
    10441 ttatattgaa tattggccct gctacaatag tactaaatct ttgtaatata gacttgtttc
    10501 caaaagaatt tggacttatt tgttcttcat cttcaccttc catgctgaca tatccaccaa
    10561 ttggaagtgc tcttatagaa tactctgtat ctttttttac actatatatc ttaggcccca
    10621 tacctataga aaactcattt actttgatac cacttctttt tgcaaatata aaatgtccta
    10681 actcatgtat taatacaatt atactaaata gtattaatgc ggctattata gtcataaatt
    10741 caccttccta tttttttatc agatttgcaa cataagctct actccatcta tctgtttcta
    10801 agatttcttc taatgttggt tcacttatac tactatgaac ttctaaagtc ttctctatgt
    10861 aatatggtat gtcatagaaa ccaattttat cctctaaaaa ttcacttact aaaacttcat
    10921 ttgctgaatt taaaacagaa gaataagttc ctcccatttt taaacactca tatgccaatt
    10981 ttaaacatgg aaaagtttct aaatctggtt cttcaaaagt taaagtacta aattttgaaa
    11041 aattcattct ttcaaagcta ctttccattc tatctggata tgtaagagca tactgtattg
    11101 gtagtctcat atctggacat cctaattgag caattattga gctatctgta tactgcacca
    11161 ttgaatgaat tatgctttgt gggtgaacta ctacatctat attttcttgt tctactccaa
    11221 ataaccatct agcttctatc acttcaagtc ctttattcat aagcgttgaa gaatcaatac
    11281 ttatttttct tcccatactc caatttggat gcttcaaagc ttcgttttta gttatatttg
    11341 caagttctcc ctttttcttt cctctaaatg gaccaccaga tgctgtaagt attatctttt
    11401 ctatattttt tttattttct ccatttagac attgaaatat agcactatgt tcactatcaa
    11461 caggaagaat atttacattg tatttttctg cttctttcat tacaagttca ccagcagtta
    11521 ccaacgtttc tttattcgct aatgctatgt ctattccctt ctttatagca caaagagttg
    11581 ggactaatcc tatcatacct actacagcag ttaaaaggac atccacttca tcaagtgatg
    11641 atattatttt taatccttcc attcctgcta aaacttctat ttctatattt ttaggcaaca
    11701 tttcttttaa ttttaatgct ttattttcct caaaaactgt tacatattta ggtttaaact
    11761 ctactatctg ttctagcaat aattcaatat tactgttagc tgatattgca actatctcaa
    11821 acttatctct atgttctcta accacatcca aagtttgttt acctatagaa cctgtagaac
    11881 ctaaaattga tatctttttc atataataat cctttctaat aataaatata ccctgtcaaa
    11941 acattataac actactaaaa caattatttt tactattcat agaaatttca tatattattc
    12001 cacagattaa aattaatcta ttattttttg tattttgtgg aatattataa aatattctag
    12061 tattattaaa tgcccttttt tatttccaca acatcttcta ttttacagtc tagtgctaaa
    12121 catatcttct ccattgtatc catagaaaca gatttatcat ttcctagtct gcctaaaaca
    12181 gaaatactta tacctgatat atttacaaat tccattttct ttatttttct gtcaattaac
    12241 aatttccata atggagcgta tgaaatcatg ttatcacctc gcaaaatata ttctatcaca
    12301 ttttatctat tttggaaaat tttcatctat ttagataaat aattatttat ctaaatagat
    12361 ttcaattata gtttattaaa tataaatatg ttaaaataaa acaaaagcat aagttcaaaa
    12421 attaaacttt atatatattt atctcattat tttactaaaa cataactata taaaaaatat
    12481 ttac
//
LOCUS       DAMJYC010000111        12362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJYC010000111 DAMJYC010000000
VERSION     DAMJYC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.115"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            289..459
                     /locus_tag="PC472_003314"
     CDS             289..459
                     /locus_tag="PC472_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2728617.1"
                     /translation="MSPKKIGRPVVGSPKTNDIKVRVDDETNEKLDEYCKKNNLTKAE
                     AIRQGIHLLLEK"
     gene            585..1391
                     /locus_tag="PC472_003315"
     CDS             585..1391
                     /locus_tag="PC472_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF2728618.1"
                     /translation="MMNDLQIFKSEDFGEIRTVDIDNEIWFVGKDIAEALGYSNTREA
                     LKTHIDNDDIAEVVIHDGSQNRNMKITNESGLYSLIFGSKLETAKNFKNWVTKEVLPA
                     IRQHGAYITNNADPQALREKANEIESLDTVNKTIEILTPFLDNAGIDEKAKLLTAKTI
                     YKKAGIELPLEIEEKEHFFDTKQIATKLNMYSKSNKPAFHAIGEIIKKLDIQDNEKLV
                     VLESKRGWSGSVNKYSQSVIDKIRHWIGENNKPAKIQGEKKNFHVVYKIE"
     gene            1448..1624
                     /locus_tag="PC472_003316"
     CDS             1448..1624
                     /locus_tag="PC472_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728619.1"
                     /translation="MIEDLLNEYNLKTDEDVEYFVKFANALYELKQNSEEKFQEYAEI
                     LRGILREQEERKNK"
     gene            2045..2239
                     /locus_tag="PC472_003317"
     CDS             2045..2239
                     /locus_tag="PC472_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728620.1"
                     /translation="MWCVKELVEYKNLKSEFSIILTKWYVNSNIKDFTSLPSIGFILT
                     MWYVKKELLRRMLLAGEILY"
     gene            2643..3425
                     /locus_tag="PC472_003318"
     CDS             2643..3425
                     /locus_tag="PC472_003318"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2728621.1"
                     /translation="MGLFGGKEPCSICGGKGKNKVLENEYLCNNCFINFTIFSRERLK
                     ATSAMQVLADHEGIRKFINFSKTNRELLAKFVETNRINKFVSIDEDNNFIKISDIRKG
                     GDVIENVYAIDEIMQFELLEDGESIVSGGLGRAVVGGALFGATGAIVGGITGKKTTRK
                     VVDTFKIKITFNNINNPLEYVNLINTKTKTNSSIYQNACNEAQEILSVLSIIIKNNDK
                     TDNTQSKTSDSIEQVKGLKELLDLGAITEDEFNTKKKELLNL"
     gene            3492..5849
                     /locus_tag="PC472_003319"
     CDS             3492..5849
                     /locus_tag="PC472_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2728622.1"
                     /translation="MATIQTSIRIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     TGGIQASQQSLNNIENILTRIEQNIGKADEQQRRFNEDINKGVSNTDRLLGSVKKITG
                     AYMSLKTLGGLGSLSDQMTNTNARLSLINDGQLSDGGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSSTREIVSFAEQLNKKFIIAGASTQEMSSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIKSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAASAETNAQFE
                     KMPLTIGQIFTSIKNNAVMIFGAIQKKIEDTVSSGGFRTFIVNVTDSLYVLGAVGYSV
                     FNGFIDLLSSPTFQGFANMMIVGISLIAQGLGWIITQALSVANVFTQNWSIIAPIVYG
                     IVGAFIAYKVVIYSVFLAQAIYTAITFTQCFVTALFAGELYVATKAMLIHQLMTLGVS
                     RANVTLCATMIMVVSAVALVIAAIFVGVAIFNHFAKTGISATGVVIGAFYFLGACIYD
                     VFAGAWNIVMAFTEFFVNSFNICIYGVQMLFYNLANFVINSMGDVGGSFDRCATTLAN
                     VFINGVNLAIKAVNGLIGLINKIPGVNIDTFGHGYTKNIDSVVGSLKNYQKTLKEPIK
                     PQDWKAPYMDIKNPFDSFNKGYKVGQNLENKLKDAFDINKIAEKAKKDLGLDDLWDKK
                     YGLGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINK
                     YTGVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGIV"
     gene            5865..6560
                     /locus_tag="PC472_003320"
     CDS             5865..6560
                     /locus_tag="PC472_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2728623.1"
                     /translation="MAYDFYLDGVQLPITPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            6553..8508
                     /locus_tag="PC472_003321"
     CDS             6553..8508
                     /locus_tag="PC472_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2728624.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYILYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDTEMANKINKLLKGKLSGTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDVTYQIQGNNQSSSTNNSSKTDKLISIAKSKLGCNYVYGAEGPNTFDCSGFTQWCYK
                     QIGIKIPRTVATQSKAGSAVDLKDRSKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHSP
                     QTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            8522..8782
                     /locus_tag="PC472_003322"
     CDS             8522..8782
                     /locus_tag="PC472_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2728625.1"
                     /translation="MSQELLQIIKKTAIDAVETSNPMQIAFGTIESLNPLVVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            8787..9206
                     /locus_tag="PC472_003323"
     CDS             8787..9206
                     /locus_tag="PC472_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2728626.1"
                     /translation="MIPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            9207..10253
                     /locus_tag="PC472_003324"
     CDS             9207..10253
                     /locus_tag="PC472_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2728627.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSTDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNLT
                     IDSNSIVVRGEVVG"
     gene            10246..10863
                     /locus_tag="PC472_003325"
     CDS             10246..10863
                     /locus_tag="PC472_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2728628.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNAENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLVSKDNY
                     MNFGATTITGHEVTIYPWSPSDIETQGEINILSGNGFGYQEVTIY"
     gene            10879..11904
                     /locus_tag="PC472_003326"
     CDS             10879..11904
                     /locus_tag="PC472_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2728629.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFVKIQLGDGGGNEY
                     NPTEEQTALKNIVWEGKVGNVKTDESMTNCLILESLIPASAGGFVVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IGTVNTKIDTTKAELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVAYTQRVA
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDVTTKENVVTNFKIIDDNSIEIRSEVKA
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLINIEEKVNGGLSSIATSV
                     NELITYC"
     gene            11921..>12362
                     /locus_tag="PC472_003327"
     CDS             11921..>12362
                     /locus_tag="PC472_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728630.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDDEQGLLSRIIVAGGGGGSYSPYTGGAGGGL"
BASE COUNT         4859 a         1383 c         2410 g         3710 t
ORIGIN      
        1 ggaacataag gtaaatcata agaaactcca acaagaattt tatattaact atgtggtatg
       61 taaagatcac tttttctcaa gctcttacaa tgatgaaaaa attttatatt aactatgtgg
      121 attcaaaatt aaataaacaa agaaagcact tacaaatatg taggtgcttt tgttttgctc
      181 aaattggtcg attgggtaaa ataattagaa aaaattggga taagttattg acttttgagt
      241 tccaaaacta tataatttaa ttatggaact caaaagtgag gtgataacat gagtcctaaa
      301 aaaataggaa gacctgttgt aggaagccct aaaactaatg atattaaggt tagagtagat
      361 gatgaaacta atgaaaaatt agatgaatac tgtaaaaaaa ataaccttac aaaagcagag
      421 gctataagac agggaatcca tttactttta gaaaaataac aaaaatagag tgttcgcccg
      481 accaagtttg aacactctat ttaaaccata agaagtaatc ttctatatga aatattttat
      541 catatagaga aacttctttc aaattaaatt taggaggagt atgtatgatg aatgatttac
      601 aaatatttaa aagtgaagat tttggagaga taagaacagt tgatattgat aatgaaattt
      661 ggtttgttgg taaagatata gcagaagctt tgggatattc taatacaaga gaagctttaa
      721 aaactcacat tgataatgat gatatagctg aggtcgtgat tcacgacggc agccaaaata
      781 gaaatatgaa aataaccaat gaaagtggac tttattctct gatatttggg agcaagttag
      841 agacagctaa aaattttaag aattgggtta caaaagaagt tttaccagct attcgacaac
      901 atggtgcata cataacaaat aacgctgacc ctcaagcatt aagagaaaaa gcaaatgaaa
      961 tagaaagttt agatacagtt aataagacta tagaaatact aacacctttt cttgataatg
     1021 ctggaataga tgaaaaagca aagttactta cagcaaagac tatctacaaa aaagcaggaa
     1081 tagagttgcc tttggagatt gaagaaaaag aacatttctt tgatacaaaa caaattgcaa
     1141 ctaaattaaa tatgtattct aagtctaata aacctgcatt tcatgcaata ggtgaaatta
     1201 ttaagaagtt agatatacaa gataatgaaa agttagtagt actagagagt aaaagaggtt
     1261 ggagtggaag tgtcaataag tattcacaga gtgtaataga taaaataaga cattggatag
     1321 gtgaaaacaa taagcctgct aagatacaag gtgagaagaa gaattttcat gtggtttata
     1381 agattgagta aatttataag attgtattaa ataatttatt ttagtttatt ttggggggat
     1441 taatacaatg attgaagatt tacttaatga atataattta aaaactgatg aagatgtaga
     1501 gtattttgtg aagtttgcta atgcattata tgaactaaaa cagaatagtg aagaaaaatt
     1561 tcaagaatat gcagagatat taagaggtat tcttagagaa caagaagaga gaaaaaataa
     1621 gtaaaataat atagataaag cacttgaata ttatgatgtt tcaagtgctt tgtttgttaa
     1681 aaaatgatat aatataggta agagttatat taacaatgtg gtatgtaaag tgactttcat
     1741 atgctctttt atcagcttct gacataagtt ttatattaac taagtggtat gtaaagactg
     1801 gtaaaactct taatgtatat gttatttgta aagattttat attaactatg tggtatgtaa
     1861 agcagctttt tcttttaagc tacataactg attatactat tttatattaa ctaagtggta
     1921 tgtaaagttc ataacttgat ttgattcata acttgtagcc attgttttat atcaactatg
     1981 tggtatgtaa attgtcttat tttattatta aagttttctt gttcatttgt gttttatatt
     2041 aactatgtgg tgtgtaaagg aattagtaga gtacaaaaat ttaaagtcag aatttagtat
     2101 tatattaact aagtggtatg taaattcaaa tataaaagac tttacatctt tgccatcgat
     2161 tggttttata ttaacaatgt ggtatgtaaa gaaggaactt ttaagaagaa tgttacttgc
     2221 aggagaaatt ttatattaac taagtggtat gtaaagtcca catctgcatc tttacttatg
     2281 ttatttgtat tgtgttttat attaactaag tggtatgtaa agtcatttat catgttcaat
     2341 ctagcattcg tactagtcag ttttatatta actaagtggt atgtaaagaa tggagctata
     2401 gtagacagca ttgatacaaa tattatttta tattaactaa gtggtatgta aagtttgcaa
     2461 tagcttgttt cataagatat acgcattgag ttttatatta actatgtgga tttaaaatta
     2521 aaaataatga aaaacactta cttgaatagt aggtgttttt ttattgaaag gatgtgataa
     2581 taatgtaaaa attttactga tatagtataa taatcttata aaattatgta gggggtaata
     2641 ttatgggatt atttgggggt aaagaaccat gcagcatatg tggaggaaag ggtaaaaata
     2701 aagttttaga aaatgaatat ttatgtaata attgttttat taatttcaca atattttcta
     2761 gagaaagatt aaaagcaact agtgcaatgc aagtattagc tgaccatgaa gggataagaa
     2821 aatttataaa tttttctaaa acaaatagag aactattagc aaaatttgtt gagacaaata
     2881 gaattaataa atttgtatcc attgatgaag ataataactt tattaaaata tcagatattc
     2941 gcaaaggtgg agatgtaata gagaatgtat atgcaattga tgaaattatg caatttgaac
     3001 ttttagaaga tggagagtct atagttagtg gaggattggg aagagctgta gttggaggag
     3061 ctttgtttgg tgctactgga gctatagttg gtggaataac aggcaaaaaa acaacaagaa
     3121 aagttgttga tacatttaaa attaaaataa cctttaataa tatcaataat cctttggaat
     3181 atgttaattt gattaataca aaaacaaaaa ctaattcatc tatatatcag aatgcttgta
     3241 atgaagcaca agaaatttta tcagtacttt caataataat aaaaaataat gataagactg
     3301 ataatacaca aagtaaaact agtgattcta tagaacaggt taaaggttta aaagagctac
     3361 tagacttagg agcaataaca gaagacgaat ttaatactaa gaaaaaagaa ttgttgaatt
     3421 tgtaatatta taaaacactt acttatgtag gtgttttttt atataaaaat tttagaaagg
     3481 agagtgaaaa tatggctaca atacaaacat caattcgaat tttcgatgga atgacacctg
     3541 cctttaggaa tatgactaat tctattaata caacaattaa tagtttagag agactgcaag
     3601 gaagattaaa taatccactt aatacaggtg gtatacaagc atctcagcag agcttaaata
     3661 atattgaaaa tatcttaact agaatagaac agaatatagg aaaagcagat gaacaacaga
     3721 gaagatttaa cgaagatatt aataaagggg taagcaatac agatagattg cttggaagtg
     3781 ttaaaaagat tactggagct tatatgagct taaaaacatt aggaggatta ggcagtttaa
     3841 gcgaccaaat gactaacact aatgcccgct tgagtctgat aaacgatggt caactttctg
     3901 acggagggtt aaataaaatg attttccaat ccgctgaaag gtctcgtgca tcatatttgg
     3961 atactgcaca gatagtatca cgaatcggaa tgaatgcagg aagtgcattt agtagtacta
     4021 gagaaatagt aagttttgca gaacaattga acaaaaaatt tataatagca ggagctagta
     4081 ctcaagagat gagttcggca cttttacaat tgactcaagg tttgggttct ggtgtgttga
     4141 gaggagagga actgaatgct gtatttgagt cagcacctaa tataattaag tcgattgctg
     4201 actacttaga cgtggatata ggaaaaatta gaggaatggc aagcgaggga atgttgacag
     4261 cagatattgt aaaaaattct ctgcttgcag catcagcaga aacaaatgct caatttgaaa
     4321 aaatgccact tacaataggt caaatcttta catcaattaa gaataacgca gtaatgatat
     4381 ttggagcgat acaaaaaaag attgaggata cagtctcaag cggaggattt cgaaccttca
     4441 tagttaatgt aacagattcg ttgtatgtct taggagctgt tggatatagt gtctttaatg
     4501 ggtttattga tttgctgagc agtccaacat ttcaaggttt tgcaaatatg atgattgtcg
     4561 gaatcagttt aattgcgcaa gggttgggat ggataataac acaagcacta agtgttgcta
     4621 atgtatttac acaaaactgg agtattattg cacctatagt ctatggtatt gtaggagctt
     4681 ttattgctta taaagttgta atatattcag tttttttagc tcaggcaatt tatactgcaa
     4741 ttacttttac acaatgcttt gtaacagcac ttttcgctgg agaattatac gttgctacaa
     4801 aagcaatgct tatacaccaa ttgatgactt taggagtaag cagagctaat gtcacactat
     4861 gtgcgactat gattatggtg gtatcagctg tagccttagt aatagcagcg atattcgtag
     4921 gagtagctat attcaaccac ttcgcaaaaa cgggtataag tgcaactgga gtagtaattg
     4981 gagcttttta tttcttggga gcttgtattt atgacgtatt cgcaggagct tggaacatag
     5041 taatggcatt tacggagttc tttgttaact catttaatat atgtatctat ggtgtacaga
     5101 tgctattcta taatcttgca aatttcgtta taaactcaat gggtgatgtt ggaggttctt
     5161 tcgatagatg tgcaacaaca cttgcaaatg tattcataaa tggagtaaat ttagcaataa
     5221 aagcagtaaa tgggttaatt ggattaatta ataaaatacc tggagtaaat atagatacct
     5281 ttggtcatgg ttatacaaaa aatatagact ctgtcgttgg aagtttaaaa aactatcaaa
     5341 aaacattaaa agagcctatt aaaccacaag actggaaagc accatatatg gacattaaaa
     5401 atccatttga ctcttttaat aaaggatata aagtaggtca aaatttagaa aacaaattaa
     5461 aagatgcttt tgacatcaat aaaatagcag aaaaggctaa aaaggattta ggactagatg
     5521 acctttggga caaaaaatac ggtcttggtg acggactcgg ttcggcaggg cttaattctc
     5581 cgctcagcga cgctgcaaaa ggagcaaaag atacagcagg aaacacagct aaaatggcaa
     5641 agacaatgga taaaagtcaa gaggacttaa aatacttaag agatatagct gaacaagaag
     5701 taataaacaa gtatacagga gtcaacatta aaattgacat gaacaataca aacaacataa
     5761 gtaaagatac tgacttagat ggaatagtta atgtattaac tgaaaaatta aacgacgcca
     5821 tggttgtatc agcagaagga atagtttaga aaggagggat ataaatggct tatgattttt
     5881 acttggatgg agtgcaatta ccaatcacac ctggcaaatt agagattaaa gtaacaaaca
     5941 aaaataaaac agttgattta ataaacactg gagaagtaaa catactaaaa aaagaagggc
     6001 tatctgaaat aagttttgaa gcagagttta cacataataa attgcctttt tgtagaggtc
     6061 aatttagaga tgttcaattc tttttaagta aactggaatt actaaagaca gattgtaagc
     6121 catttcaatt tattgtttca agggaattag gtaataaagt actatttaac actaatatga
     6181 aagtgtcatt agaagaatat aacatagtag aagatgcaga gaatgcctca gatacaaaag
     6241 ttgcaataaa gttaaaacaa tatagagatt actcaactaa aaagttagtt cctgctccac
     6301 ctaaaaaaac taatgagact ggtagaccaa gcgtaaagat agagccaaaa cgagttgatt
     6361 cagtcaatgc accaaatgct aaaacataca cagtcaaggc aggggatagt ctttggtcaa
     6421 tttgccaaaa acagttaggt aatggctcac tgtataagaa ggtatatgag ttaaataaat
     6481 caatgatgga taaggctaac aagggcaaaa aagtacctaa atataccatc tataaagggc
     6541 aggtgttgag gcttgtctga tgaattagtt ttggcaaatg atagggatgt aagattagtc
     6601 atagcacatt gggaagattt ctacgaacct gtagttttgg atggtatcac atgggaaatt
     6661 gaaaggcgag gaacaccttc aaagttagaa tttacaatag tcatggatga tatattagag
     6721 ttttgcgaag gtaactcagt aagattatat tacaaaggtg taggcatatt ttatggatat
     6781 atatttcaga agaaaagaga taaagaaaat cacattaaaa ttgttgctta cgaccagcta
     6841 agatatttta agaataaaga tacttatgta tatagcaata aaactgcaag tgaacttgta
     6901 aaaatgttgg ctaaagattt taatttaaaa tacaatgtca tagaagatac taagtataaa
     6961 atatctagga tagaagaaaa taaaacactc tttgacatga tactaactgc acttgatgat
     7021 actttaagag agaaaaaaga aatgtatatc ttgtatgatg attttggaag aataacatta
     7081 aagaatgttg catcaatgaa actggatact gtcatgaaca atgatgtaat tgaggatttt
     7141 gactataatt catcaataga tagtgatact tacacaaaga ttaaacttgt gagagataat
     7201 gaagaaacag ggaaaagaga tgtatatatt gcacaagact ctacacatat gaggagttgg
     7261 ggaatactac aactatttga tacagtagat aaaaacatga gtgaagcaga gataaagcaa
     7321 aaatgtgata tacttctaaa actatataat aagaaaacta agtcattaag tttaaaaaat
     7381 gtgttaggag atattagagt aagagcaggt tgtttagtac ctgttttttt agatttagga
     7441 gatattgatt tacaaaatta tatgttagtt gagaaagtaa agcacacatt tgaaaataat
     7501 tcgcacttta tggatttgac tcttgttgat ggagatgaat ttgcttctta ttcttcaagc
     7561 tcatatagta gtggaaatac taataataaa gatgaaaaga aaaatggtcc tgcacaaagt
     7621 actacaagta aagaagatac tgaaatggct aataaaatta ataaactact taaaggtaaa
     7681 ttatcaggaa caggaaatat atttgttaaa tattcaaatg cttataaagt taatccagca
     7741 ttaatggcgg ctatatctat gcacgaatca gctagaggga cttcaaatat tgcaaatact
     7801 aaaaataatt tctttggaat gaaaaaaaat ggagattaca tgagtttttc tagtgtagac
     7861 gaaggaataa aaagaggtat aagtaattta tcaagaaact atatccatat aggacgaaaa
     7921 actttagaaa gcatcagaaa taaatattct tctagttcag acaaagaatg ggtaaaatgt
     7981 gtaggtgcat tttataagca aataacagga agtacttata attctaatag tgcaggtaca
     8041 ggagttggaa gtaacgaaga agcagaaaag aatttaaaag atgtaactta tcaaattcaa
     8101 ggcaacaacc aaagcagtag tacaaataat agctctaaaa cagataaatt aattagtata
     8161 gcaaaaagta aactgggttg taattatgtg tatggagcag aaggtcctaa tacttttgat
     8221 tgttctggat tcactcaatg gtgttataaa caaataggta taaaaattcc taggactgtt
     8281 gcaacgcaaa gtaaggcagg cagtgcagta gatttaaaag atagaagcaa gtggaaagca
     8341 ggagacttat tatgtagagt tggtggaggt agtagtaatc acgtaatgat gtatattgga
     8401 aatggtcaaa tgattcattc accacagact ggtgatgtag taaaaataca atcagttgat
     8461 tcatatagaa aaggaaaagc atatacacat gtgagaagat ttatataagt gaggtgataa
     8521 agtgtcacaa gaattattgc aaataattaa gaagactgca atagatgcag tagaaacaag
     8581 caatccaatg cagattgcat ttggaactat agaaagtctt aatccattag tagttaagat
     8641 agaacaaaaa ctatctattg gtgaaatttt tctaatacaa acagatacat ttaaaagata
     8701 tacagataaa aagataggag ataagttagt tttaattcgt atgcaaggag ggcaacaata
     8761 cttgatttta gataggatgt gatgaagtga taccaacaga taacattgac tatgatattg
     8821 aagatgtctc aataattaat tttgatgtta gacaagaacc aagtaagacg tttaaattga
     8881 atatagagaa aaatagagta gatggtattt gtgatgatgt agaagcatta aaacaaacca
     8941 tctttttgat tttaaacaca gagagatacc aacatctaat ctatagttgg aattatggag
     9001 tcgagttgaa cgaccttatt ggagaaccta tttcatatgt aatacccgaa cttgaaagaa
     9061 ggataacaga agcactaatt caagatgata ggattgaaaa tatagataat tttgagtttc
     9121 aaaatataaa gggtaaagta caatgtagat tttcagttca tacaaaatat ggaaatataa
     9181 aagcagagaa ggtggtgagt gtataattgt ttgagttaat gacatttgaa aatataatta
     9241 aaagaatgtt agatagtgta ccagatactt ttgataaaag ggaaggttct ataatatata
     9301 atgctcttgc tcctgttgct atagaactta cagaaacata cattgcaatg gatgaattac
     9361 tagaccaaac attcgtagat actgctagtt attactattt agagaagaga tgtaaagaga
     9421 gaggtattac acctttacct gcaaccaata caattgcaaa aggagttttt aatatagata
     9481 ttcctattga ttcaagattc aacttaggag aatacaatta tgtggcaatt gagagaatat
     9541 ctgaaaaaac atataaaatg aaatgtgaaa ctactggacc tgtatttgaa cttggtcagt
     9601 tgattccaat tgaatatgta gacaaattag aaactgctga gttaactgaa atactaatta
     9661 atggagaaga tgaagaatca gaggacagtt taaggcaacg atattatgat agtctaaatt
     9721 ctcaatcttt tggggggaat atacaaaact ataaggatga agttaataaa ttgcctgacg
     9781 ttgggggagt taaagtatat ccagtttgga acggtggagg tactgttaag ttagtaataa
     9841 ttaattcaaa ttttaaagta ccaagtactg atttagttaa tttagttcaa gaagaaattg
     9901 acccaaatat gaaaggagaa ggtttaggat tagcaccaat aggtcataga gttacagttg
     9961 aaggagttac aagtacaact ataaatatat cagcagaaat aacatataag agtggatata
    10021 catgggagaa tataaaatca attgcagaag aagcaataga cgactattta aatgagttaa
    10081 atatgagttg ggaagatgaa gaaaacttaa tagtccgtat atctcagata gaaactcgtt
    10141 tgttaagtat agatggagta ttagatattg caaatacgat gataaatagt attgcatcta
    10201 atctaacaat agatagtaac agcatagtag tgagaggtga ggtagttgga taaagagatt
    10261 aatctaataa attacttacc acagatttta caggataaag aagaatatat aaaagtattt
    10321 aatgcagaaa acaaagaaat aaaaacacta catgataaat taaaggacct atcaaatgac
    10381 cagtttttag aggacctaac tataagtggt ataaaaagat gggaaaagat aatgtctata
    10441 actcctaaaa gtaatgagag tttagaagat agaaggttta ggatttttag taaatatata
    10501 agtaaactac cttactcaga gagattttta aggaactggc tagataatgt agttggagaa
    10561 ggcaattatg aattaactat taataatgct acttataaca tacaccttga gagtgatgct
    10621 agaaatcaag attggtttga ggaagttcat tcttttgtaa gtaatattaa gccatgcaac
    10681 atgacactag attacactag agttttagtt agcaaagata attatatgaa ttttggagca
    10741 actactatta cagggcatga ggttacaatt tacccatggt cacctagtga tattgaaaca
    10801 caaggagaga ttaatatact cagtggcaat gggtttggat atcaagaggt tacaatttat
    10861 tagataggag gtggtagttt ggctacagat aaaagttact acacaatatt aacagacata
    10921 ggaaaagcga agattgcaaa tgcaagctta gtaggagaga aagttgattt tgttaagatt
    10981 caacttggag atggtggagg aaatgagtat aatcctacag aagaacaaac agcattaaaa
    11041 aatatagttt gggaaggtaa agtagggaat gtcaagactg atgaaagcat gacaaattgc
    11101 ttaattttag aaagtttaat acctgctagt gctggtgggt ttgtagttag agagataggt
    11161 tatttagata ccgaaggtaa tttactagct atatcaaagt ataggtcagc atataaacct
    11221 aaagtggaag atggagcagt aattgacatg aaagtaaaaa ctatctttgt tgtatctaat
    11281 gtaaataata tagaactaaa gatagatcca actataatct ttgccacatt aaaagattta
    11341 caggacctag acactaaaat aggtactgtt aatactaaaa tagatacaac taaggcagaa
    11401 ttaacaagca acatagaaac tgctaaaaca gagttaaata ctagaattga cacagaaaat
    11461 gagaaacaaa atattaaaat tgaccaatta attgcaggtg gttcaaatgt tgcatacact
    11521 caaagggtcg caattgatga ttgggtggag gatgcagaaa atggattcaa agcaactgta
    11581 acacatagtt tattaacaca gagaatagtt gtaaatatta tagatgttac tacaaaagaa
    11641 aatgtagtta caaactttaa aattatagat gataattcta tagaaattag aagtgaagta
    11701 aaagctgagt taaacgttta tgtgataaat ggaaatgcag aaactcattt tattaatgcg
    11761 actgtggatg ataacagagt gtctgaaatg actacttatt cgtctaagaa aatcgaggat
    11821 agattgatta atatagaaga aaaagtaaat ggtggtttat ctagtattgc aacaagtgta
    11881 aatgagttga taacttattg ttagagagga gagtgagaaa atgcagacag aatggaactt
    11941 tgggtacaat ggttcgccac aaagtgttat attgaaacct ggcaaatata aatttgaatg
    12001 ctggggttct tctggaggta tcaacaattc ttcttggcat actgatgcta aaggcggata
    12061 ttctaaaggt gaaattacat taaaaaaaca aactacatta tatgtttacg tcggcgaaag
    12121 tggttttgct tcttcatcta cgagtaataa cactaaaagt ggttttaatg gcggtggtaa
    12181 aggttattta aatcaacagg ttatgggtac ttattattct atgtacggtg gtggtgctac
    12241 cgatataaga cttgttggtg gtgcttggga tgatgagcaa ggtttgctat ctcgtataat
    12301 tgtcgcaggt ggtggcggtg gttcatattc tccttatact ggtggtgcag gaggaggatt
    12361 ag
//
LOCUS       DAMJYC010000112        11838 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJYC010000112 DAMJYC010000000
VERSION     DAMJYC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.116"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            266..730
                     /locus_tag="PC472_003328"
     CDS             266..730
                     /locus_tag="PC472_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2728631.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            737..1231
                     /locus_tag="PC472_003329"
     CDS             737..1231
                     /locus_tag="PC472_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2728632.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNVFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1247..2272
                     /locus_tag="PC472_003330"
     CDS             1247..2272
                     /locus_tag="PC472_003330"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2728633.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2259..4706
                     /locus_tag="PC472_003331"
     CDS             2259..4706
                     /locus_tag="PC472_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2728634.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4886..6259
                     /gene="radA"
                     /locus_tag="PC472_003332"
     CDS             4886..6259
                     /gene="radA"
                     /locus_tag="PC472_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2728635.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6262..7332
                     /gene="disA"
                     /locus_tag="PC472_003333"
     CDS             6262..7332
                     /gene="disA"
                     /locus_tag="PC472_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2728636.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7421..8515
                     /locus_tag="PC472_003334"
     CDS             7421..8515
                     /locus_tag="PC472_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2728637.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8644..10821
                     /locus_tag="PC472_003335"
     CDS             8644..10821
                     /locus_tag="PC472_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728638.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(11007..11777)
                     /locus_tag="PC472_003336"
                     /pseudo
     CDS             complement(11007..11777)
                     /locus_tag="PC472_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4903 a         1216 c         2129 g         3590 t
ORIGIN      
        1 caaatgttta gtaataaaga tgagaaggta tatttaaaat ataaagtatt taaagttctg
       61 aaatctaatc atgaagcaaa aaattagcat ttggttatca ataaatataa taaataaaaa
      121 ctcataatta atgaaagaat aaattcatat ctaaatttct ataaaaaatt taaatttata
      181 caatttaggg ctttaaaaat ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa
      241 aataaaaagg tgtgtggtga tattaatggc aactatgaca gatataattg aaaaatttat
      301 taaagatttg atggaagaag ataatagcat acaaattcaa agaaatgaac tagcaaactt
      361 gtttagttgt gcaccatcac aaataaatta tgtcttaaca acaagattta caatagatag
      421 aggatattac attgaaagta aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg
      481 aaaaagtaaa gatgggcata ttagagaact actaaatgaa aaaataggaa gtcagatatc
      541 ctataaaaaa gcaaaagaat tattagaagg tttaaaagag tcggatttga ttaacgaaag
      601 agaattaaaa ttaatattat atgcaataga tgataaatca ttgtgtatgc caatatatga
      661 attaaaagag aaggtaaggt ctaatatttt aaagaatata ataataggat tatttagtat
      721 agaggagtga taatttatgc tatgtcaaaa atgcaataag aataaagcat ctgtatatta
      781 taataagata gtcaatggag aaaaaacaga gatgtattta tgtagtgagt gtgcaaaaga
      841 gaatacagaa atgaatttta acttagatat gcctttttca atgatggata tattttctaa
      901 tttaggtttt caacctaaaa aagaacttga ggaaaagcta gtttgtccta aatgcaatac
      961 aacatatagt gagtttaaga ataatgggag atttggatgt agtgaatgtt ataatgtatt
     1021 tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg catatagaac atacaggaaa
     1081 agcacctaaa aaatcttttt ataagataag tgtagaaaat gagattaaag aactaaaaga
     1141 agatttagat agagctataa aaaatgaaga gtatgaatta gcagctcaat ttagagataa
     1201 aattaaatat ttaaaaggtt ctattgacta agattggagt gatattatga aagagaacat
     1261 agtcatgaaa tcaagagtta ggctagctag aaatttaaat aactatccat ttccaaataa
     1321 attagataaa gaatgtgcca tggaaataat agaaaaagta aaaaatgcgt ttattaattc
     1381 taacttagaa caaaaagaag aatttgattt ctataaaatt gaagatttag accaatctaa
     1441 aaaaatgctt atggttgaag aacatataat aagcccagat ttagcagaaa atgataaaag
     1501 tgcagttata gtaaaaaaag ataaaactat aagtataatg ataaatgaag aagaccatat
     1561 aaggatacaa actatatgtg atgatttaaa cttagaatat gcatatagtg tagcaaatga
     1621 aatagatgat ttgttagaat caagtttaga atatgccttt aacacaaagc taggttatct
     1681 aacatcttgt ccaactaata caggaacagg aatgagagct tctgtaatga tgcatttacc
     1741 agcattaagt caactaggct atatggatga actttataaa atttcatcac aaataggtat
     1801 agcaattaga gggatatatg gagaacgaac agaagcattg ggaaatatat atcaaatatc
     1861 taatcaactt acacttggaa gaacagagtc taatattata gaaaatgtaa gtggacttac
     1921 aaaagatgca atttcaaaag aaataaaagc tagagaaata ttgcaaaaaa aattaggtat
     1981 aaaactagaa gataaaatat ttagatcgat agggacactt gaaaattcaa gagtaatgag
     2041 tagcgcagag gcaatgagcc atctatcaaa tataaaaatg ggaatagaaa tgaactatat
     2101 agataaacta gatttaaggg ctatagaaca acttatgata ggtatacaac cagcgcatca
     2161 gtcaataatg tataagagtg atgatgttga gaatagagat ataaatagag caacatatat
     2221 tagagaaact ttggaaaaat taagaggagg gatgaattgt gaacttcaat agatttacac
     2281 aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc taaaagtcta ggacacaata
     2341 ttgtcggaag tgaacatata cttttaggtc ttttaagaga agaagaaggt atagctgcta
     2401 aagttctaag taaagttgga tttacagaag cttatttaga aggcaaaata gttgatatgg
     2461 aaggtaaggg agaagaaatc tcagaagata tagtattaag tccaagaagt aaacaaatac
     2521 ttgaattatc aggaatgttt gcaaataaat taaaaacaaa ttatattggg actgagcata
     2581 tattattagc aattattcaa gaaggtgaag gaatagctaa taagatttta aattatgctg
     2641 gagtaaatga taggacttta gcccaattaa caattgatat gatgggtatt agtgataaaa
     2701 atcaatacaa agcagaaaat agttacacgg gcaaccagaa tcaagcagaa tctaaagtac
     2761 ttgataagta tggaagaaat cttacactat atgcaaaaca gaataaaata gaccctgtaa
     2821 taggtagaga aaaagaaata caaagagtaa tacaaatatt aagtagaaga actaaaaaca
     2881 atccagtgtt gataggagac ccaggtgtag ggaaaacagc catagctgaa ggtctggcta
     2941 caaatatagc cttaggaaat gttccagaaa cactaaaaaa caaaacttta tactctttag
     3001 agatgggttc attattggct ggggctaagt atagaggaga atttgaagaa agaattaaag
     3061 aagttgtaga tgaagttgtt aaaaatggaa acataatctt atttatagat gagatgcaca
     3121 caataatagg ggctgggtct actggagaag gctctataga tgcttcaaat atattaaagc
     3181 cagccttagc aagaggagaa atacaagtta taggtgcaac tacaattgat gaatatagaa
     3241 agcatgttga aaaagattct gctctagaaa gaagattcca accagttatg gtagatgaac
     3301 caactaaaga ggactcaata aaaatattag aaggattaag agataaatat gaagctcatc
     3361 ataaagttaa aataactgat gatgctataa aggcagctgt agaattatca actagatata
     3421 tatcagacag atatctgcca gataaagcta tagacttgat tgatgaagct gcatcaaaag
     3481 taagattaaa agaaaataca cctccttcag aaataaaaaa attagaacta gaaatagaaa
     3541 atatagataa agaaaaagaa gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa
     3601 taagagatga acaaggtcta cttaaaaaac aattagaaga tgttagagaa agatggaaca
     3661 agtcatctaa acattcagat ttagttgatg gagaagttat tgctgaagta gtgggcttat
     3721 ggacaggaat accagttaat aaaattcttg aagaagaggc tgatagactt ttgagacttg
     3781 aggaaatact gcacaataga gttataggtc aagaacaagc agtaaaatct atttcaaagg
     3841 caatcagaag gtcaagagca ggtcttaaag accctaatag accaattggt tcatttttat
     3901 ttttaggtcc aacaggagta ggtaaaacag aattatctaa ggcattagca gaagtgcaat
     3961 ttggagatga aaatcaaata attagaatag atatgtctga atatatggaa aaacatgctg
     4021 tgtcaagaat gataggttca cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa
     4081 ctgaaaaagt aagaagaaat ccatattcag ttatattatt tgatgagata gaaaaagctc
     4141 atccagatgt atttaatatt ctgttacaaa ttctagatga tggtagacta actgattcaa
     4201 aaggaagaac tgtagatttt aagaatacta ttgtaataat gacatcaaat gttggagcat
     4261 ctacaattgg tagacaaaaa actttaggat ttagtatagc taaaggagac gaagaagaaa
     4321 aatctcaata tgaaaaaatg aaagaaaata taatgggtga attaaaacaa agattcagac
     4381 cagagttttt aaacagaata gatgatataa ttgttttcca ctcactaaat gaaaatcata
     4441 tatcaaaaat agtactttta atggcagcga aactacaaga gagattaaaa gaaatggata
     4501 taaaattaga aatgagtgat gaggctgtta aattaatttc taaatctgga tttgacttag
     4561 aatatggagc aagacctctt aaaagagctt tacaaaaaga gttggaagat gagctatctg
     4621 aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt agtagcaaaa gtcaaagatg
     4681 aaaaaatagt ttttgaaact aaataattta atagagttgc attttaaaat attgcaaata
     4741 taagttttat aatctaaaca aaattttaag ggcaatgaat ctataataat tcattgtcct
     4801 taattatttt atttaatctt tacatattgt ctttcagata tgctaaaatt atagtataga
     4861 ataaaaaatt aaggatgata aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc
     4921 ttgcggatat gaaacagcta aatggcttgg taaatgtcca gaatgtacaa aatggaatac
     4981 atttgtagaa gaaatagacc aaaaaagcac caaaaaagag gtctttataa tagataaatc
     5041 ttcatctaaa ccagtcagta taaactcaat agaaagtaaa gaagaagagc ggtttacaac
     5101 agatataaat gagttagata gagtgcttgg aggaggtata gttaaaggct ctttagtgct
     5161 tgttggtggt gaccctggta taggtaaatc aacactttta atccaagtat ctagtaatgt
     5221 tgccaattta ggaaaaactg ttttatatat aactggagaa gaatcagaat ctcaaataaa
     5281 aatgagagct aaaagattag gtattaactc agaaaattta tatatatttg ctgaaaataa
     5341 tttaagtata attgaatctt atttagaaag tgtaaatcca gaattaataa ttctagactc
     5401 aattcaaact gtcttcagtc cagaaatatc ctcggcacca ggaactgtca gccaaattaa
     5461 agaaggtact tcaaaattca tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt
     5521 tgggcatgtt acaaaagaag gttcactagc aggaccaaaa ttattagagc atatggttga
     5581 tactgttcta tattttgaag gagaaagata taatacttat agattagtaa gagctgttaa
     5641 aaatcgattt ggttcaacta atgaacttgg agtatttgaa atgagggatt taggattagt
     5701 agaacttgat aatccatcta aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc
     5761 agtaataata tctactgtag aaggtacgag accaatgtta cttgaattgc aagcattagt
     5821 ttctcctaca agttttggta taccaaaaag aacatctaca ggagtcgatt acaatagagt
     5881 aggtatgtta ttagcagtat tagaaaaacg tgttggtttg caaatacaaa atcaagatgt
     5941 ctatataaac atagttggag gaataaagat aaatgaaccc tcaattgatt tagggatagc
     6001 catttctgta gcatctagtt ttagaaatat accgatagat gaagatatag ctgttacagg
     6061 agaagtggga cttacaggag aagttagagc agtaagcttt atagaaaaga gaatagctga
     6121 gtgtaaaaaa cttggattta aaaagatagt agttcctaga agtaattatg atgttgtaaa
     6181 agagaccaaa ggcatagaaa tatggccagt agataactta agacaagcta taaatatagt
     6241 acttgggagg aatcaataaa tatggagaat tttctagata ataaaaatat gctatatgca
     6301 ttaaaaatga tatctcctgg aactccactt agattaggtc taaacaatgt actaagagct
     6361 aagactggtg gattaattgt aattgcaaca aacgaagatg taatgaaaat agtagatgga
     6421 ggatttgcta taaatgcaga atattcacca tcatatctat atgaattagc taaaatggat
     6481 ggagctatag ttttaagtgg tgatgtaaag aaaatattat ttgctaatgc acaacttata
     6541 cctgactatt ttatagaaac atcagagaca ggaacaagac atagaacagc agaaagagta
     6601 gcaaaacaaa ctggtgctat agtcatagga atttcacaaa gaagaaatgt tataacagtt
     6661 tatagaggaa atgagaagta tgtagtcgaa gatatatcta agatatttac taaggcaaat
     6721 caggctatac aaactctgga aaaatataag acagtattgg accaagctgt aacaaattta
     6781 aatgccttag agtttaatga tttggtaact atttatgatg ttgcattagt catgcaaaag
     6841 atggaaatgg taatgagagt tacaagtata attgaaaaat atgtgataga attgggtgat
     6901 gaaggaactt tagtaagtat gcaattagaa gaattaatgg gtacaaccag aatagaccag
     6961 aaattaatat tcaaagatta taataaagaa aacacagaaa taaaagaact tatgaaaaag
     7021 gtcaaaaatt taaattcaga agaactaata gaattggtca atatggcaaa actattaggg
     7081 tatagtggtt tttcagaaag tatggatatg cctataaaaa caagaggtta taggattctt
     7141 agcaaaatac atagactacc aacagcaata atagaaaact tagtaaatta ttttgaaaac
     7201 tttcaacaaa ttttagatgc atctattgaa gaattagatg aggttgaagg aataggtgaa
     7261 ataagagcaa catatataaa aaatggactc ataaaaatga aacaattagt cttattagat
     7321 agacacatat gatgaatttt aaatgaattt tactactttt ttcttgaata taatatacac
     7381 tttggtctat aatagataag tataactagg gggtgaagca ttgataagga aagtaacaag
     7441 aatattgttt attttacttg gatttacaat aggtataaca acctatttaa cattaatgaa
     7501 agattttgaa atattaacat ttggaaaaga aacatatggt tatatcgcag ctgtagtagc
     7561 aggtataata atagccattt taggatatct tatagaacca tgggttgtaa ataaagtaaa
     7621 agaaattgct aaaatagtgg ataaagaatt gtcaaaatat cctcagactg atatattgtt
     7681 agggtctatg ggtcttatag taggatttgt tattgcgtat ttattaagtg gattagtaaa
     7741 tagaatacca atagtaggtg gaatactatc cttactatta tatttattcc taggatatct
     7801 tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt aatattggaa aactaggaag
     7861 attagcaaat cctattaagg ataaagataa ggataaagaa aataaaaaag aagtaaaagc
     7921 cattcctcca aaagtattag atacaagtgt aataattgat ggaagaatag ctgatatttg
     7981 taagactggg tttatagaag gaaaacttat tattcctgct tttgttttgg aggaactaag
     8041 acatattgca gattcttcag atgatttaaa aagagtaaga ggtagaagag gtctggatat
     8101 attaaatata attcagaaag aattaaatat agaagttgaa ataagtgaga gagactttga
     8161 tgatatagca gaagttgata gtaaattatt aaagcttgct caggttttaa atggaaaagt
     8221 tgttacaaat gattataact taaataaagt tgctcaattc caaggtgttg aagtattaaa
     8281 tataaatgag ttagctaatg ctataaaacc agttgctata ccaggtgaag atatggttgt
     8341 tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt gcatatctag atgatggaac
     8401 tatgatagtt gttgatggag gaagaaaata tatgaatgag actataaaag tactcgttac
     8461 ttctgttctt caaactcctg caggtagaat gatatttgca aaacctaaaa attaatatgt
     8521 tcaaagaaac ctataatttg ggctgtttat tttaaataga tggtccaaat ttatttattt
     8581 ataacgataa tgtttaatgt attcttaaag atggggattt aaatcagaaa gcagggagaa
     8641 tttatgagaa aaaagattat atctttctta gcaacattta tcataatatt aacatcagca
     8701 agtcagtata gttttgcaga tgatatatca acacgtggaa aagtaatatt tatagatatg
     8761 aatagaacta gtatgagtaa tatgttaaga ataaaatcat taagagaaga actagacaat
     8821 agagggtata ttggcttgat gaatataaga ggcgataaag gaagtgatga tagaaggtca
     8881 tatgctagta tgggtgctgg tggtagagct aatgtggcta atgaagaaga tattaatttt
     8941 gaaagctcga gtaaggatag aaatatagtt tttgaatcag ctacaggaaa aagtgcaaaa
     9001 ggtataaata atttaactat aaataagtct ataaatgaaa atttaaactt tggagagtat
     9061 ggttctgtat tagggtcatt aggacaaagt ttatctgata atggattaaa agcatctgta
     9121 ttgggtaatt cagatataat tgaaaatggt caacttataa aaaatagaaa cttatgtcta
     9181 actgcaatgg atgaatatgg aagaatacct aatggaaatg tagatactat aaataaaaaa
     9241 gacttatcta tgccttatgg aatatcaact gattatgata aattgattgt agagactaaa
     9301 gaagcttaca aaaataatga tgtgattttt gtggagcttg gagacacata tagattagat
     9361 ttatataaac caaatttaaa tgaaaaaaca tatgagagta tgaaagataa catagaaaaa
     9421 aatatagata tatatttaaa aaatatattt tctatggttg aagaaaatga cactgtctac
     9481 atagcaagtg catttccaag tgatttagat tataaaaata aaagaagact ttcgccaata
     9541 ataaaattaa atggagaagg gaaaggtata ttatcatctt caactactag aagagaaggt
     9601 attgtaacaa atttagatgt tggggttgaa atattagaca actttaatat aaaaaatcaa
     9661 aatatggttg gaagaaaata tgaacttatt aacagagatg ataataaaga atttttgatg
     9721 gatgaatatc aaaagatagt atctatttca agcataaggt ctacaatact gaatggtttt
     9781 gtaagtatag ttttcttgtc atggatagtt gctatgattg caattttatt tagaaagcat
     9841 atatcaaaaa attataaaga aacaacattt tttatattaa aggaactttt aaaaatagga
     9901 atagtaatgc cactgtcatt tatgataaca ccaataatga atttcaaaac accattagca
     9961 atatctttag gaataattat aataacactt actatatatc ttataagtaa agttttaata
    10021 aagaacgatt taaaaaacat gctgtttttt actggattaa ccatagtaat tatggttatt
    10081 gatgctgggt ttggctcata tttaatgaaa agcaatgtta tgagttatga ttgtataatt
    10141 ggagctagat attatggagt aggaaatgag tatcaaggtg tggcaatcgg tagtgcaata
    10201 tttacatttg caattttact tacatataaa aatataccaa agtggtctat aatagttttt
    10261 tctttggtta tcttaattac aagtgcatct ccaattatgg gagcaaatgt tggaagtgct
    10321 atttctgaat gtgttgcatt tttactattt atactattga tatataatgt aaagatagat
    10381 ttcaaaaaaa tcgtattatt aggtatagca gtattatttg tattaggagt ttttgtagca
    10441 atagatatga ttttgggaag taattcacat ttaggtatgt ttgtaaaaga aatttacttt
    10501 aatggaccag gagaaataat acaaacattt agtagaaaga tagagatgaa tttaaagcta
    10561 gctcaaacca gtgcatgggt aaatatactg ttaactggaa taggtgtaat tttagttctt
    10621 atgattaatc agattagata ctttaagcaa ctgatggatg agtatccaat tgtatttaaa
    10681 ggatttatag catcaatagc aggatgttta gtaactcttt tagtgaatga ctctgggatt
    10741 gtgtcatcag ctacagcctt tatttatgta attgtaccta tgataacatt aagtataaat
    10801 ctcacagcat taaaagaata agtactgtat gaataatatg taaatattaa tacattaatt
    10861 taacttcttt atttacaaaa taaagaagaa agtttataaa aaataaatta taataaaaaa
    10921 gagtttacct caaaattgat tattctaatc aaattttaag gtaaactttt tttattataa
    10981 agataaataa catataacct catattttag gtagattttt ttcttatatg tttaggagtg
    11041 catttaaatt tttccttaaa aattctgtaa aataattttg tattagtaaa tccatgagct
    11101 tctaatatac tgcataaggt atcatttgtg ttaattaaat ctttataaat atgagaaata
    11161 cgcacatcat ttaaatactc taagtaagtt tgtcccatat actgtttaaa ttttctacaa
    11221 aaatattctg gttgtaagtg tgctacctta gcgattttgt taatagaaat aggcattttg
    11281 taattaatct ttgtatattc taaaactggc tcaaattttg acaaatcaag tactgttttt
    11341 ttcatatttt tattacctac agatatttta aagtttgtat ataattcaaa taaaagttca
    11401 aataataagc ttgtaaagag cagattagct gcaggaggag aactaagttt aatttctctc
    11461 atgttttcta atataagttt aactttactt gaattttttt tatagttatt gtcattggca
    11521 ttaaccttaa agtcaaaata gcaattttta aaatcaggga tatatttgtt tagaaaagac
    11581 aatggtactt gcaataatat agaggtattt tgataaatac atctagttga gtgaataatt
    11641 ccagaattaa tgaacataca ctcattagct tttaaattat aagtgtaacc ctccatgtta
    11701 atttgtaaat caccagaagt tatataaatt atttctaatg agttatgcca atgatttgct
    11761 acataactac cactatcctg aacctcccat gactaaagtc acagggttcc tgcttcacag
    11821 aattttgcat actaaaat
//
LOCUS       DAMJYC010000113        11633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJYC010000113 DAMJYC010000000
VERSION     DAMJYC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.117"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(178..816)
                     /locus_tag="PC472_003337"
     CDS             complement(178..816)
                     /locus_tag="PC472_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2728639.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            complement(816..1679)
                     /locus_tag="PC472_003338"
     CDS             complement(816..1679)
                     /locus_tag="PC472_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728640.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            complement(1676..2050)
                     /locus_tag="PC472_003339"
     CDS             complement(1676..2050)
                     /locus_tag="PC472_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2728641.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            2417..2821
                     /locus_tag="PC472_003340"
     CDS             2417..2821
                     /locus_tag="PC472_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2728642.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            2838..3434
                     /locus_tag="PC472_003341"
     CDS             2838..3434
                     /locus_tag="PC472_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728643.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            3471..6065
                     /gene="clpB"
                     /locus_tag="PC472_003342"
     CDS             3471..6065
                     /gene="clpB"
                     /locus_tag="PC472_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2728644.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6261..7688)
                     /locus_tag="PC472_003343"
     CDS             complement(6261..7688)
                     /locus_tag="PC472_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2728645.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            7829..8725
                     /locus_tag="PC472_003344"
     CDS             7829..8725
                     /locus_tag="PC472_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2728646.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            9390..9530
                     /locus_tag="PC472_003345"
     CDS             9390..9530
                     /locus_tag="PC472_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728647.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(9527..9898)
                     /locus_tag="PC472_003346"
     CDS             complement(9527..9898)
                     /locus_tag="PC472_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728648.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            10447..10704
                     /locus_tag="PC472_003347"
     CDS             10447..10704
                     /locus_tag="PC472_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728649.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(10814..11494)
                     /locus_tag="PC472_003348"
     CDS             complement(10814..11494)
                     /locus_tag="PC472_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2728650.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT         4731 a         1306 c         1609 g         3987 t
ORIGIN      
        1 ataaaatgaa taaattatat aaataagatt agaaagtagt aaatagtctt attaaatata
       61 agtaataaaa taataaatac ctagtagaga ttaatagtta aaaaataaaa tactaccaaa
      121 ataaactcga tagtatttta tttaaatact taaatacaga aatatatata gtatatttta
      181 tacttctttt ttactataga tttttgatga tataaaatat gaaatcacaa atagtaaaat
      241 agcaacaaat ggaagagaat atactagtgt attaagtata atttctatag aaataggtga
      301 actaatattt tctaaaatac ttttaaaaac taataaagct agtgttggaa ctaaaaagca
      361 taaaatcatt aataaacgag ctctttctgt accaaattta taaataagtg gtagaataag
      421 gctaccaaaa catactgaaa ttgataagat tagtgcatta ataagtagtg tttcatttaa
      481 tataagtgta tttttaaaat aaccaatagt tacactaaca ataagtgaaa caagaacacc
      541 tataaagcta aatattaaca tagttaaata tttacttaat acaatatcat tgcgattaat
      601 aggcattgta agtacataac tatcccattt attcaaatca tcatatgaaa atgttgaaac
      661 aatcattgta gtacataaaa ctataataac acctataaag ttattaacat ttccatctcc
      721 taaagaagac ataaagccaa acataatgat gaataaaagt ataaacttta tatttccttt
      781 caaattaagt aaatctttta aaattagacc tttcattatt tatcacctct cacataaaat
      841 aacataattt cttctataga cgtattatca acaatacagt ttggatgtct tgagataaat
      901 ctatgtttat ctttaagaag tacttcatat ccaaattggc ttttacgata atatgaataa
      961 tcctctcttg aaaggttatc aaaatcacta ggtttacatt ttaaaactcc catagtatca
     1021 attaattcat ccttattttc actaaataca atttttcctt tatgtataaa tgtaatatag
     1081 tctgctatct tatctaaatc acttgtaata tgtgaagata agataataga gtgttcttca
     1141 tcttgtataa aatctaaaaa tatatctagt atttcttcac gaacgatagg gtcaagtgaa
     1201 ctagttgcct catctagtat taataatttt ggtttatgag ataaggctac agcaatacta
     1261 agtttcatct tattcccctt tgaaaactcc ttaattaatt tattgtctgg tactctcaat
     1321 ttttttaagt agtctttaaa taaggtgtca ttccaatttt tatagatttt actcatgatt
     1381 tttgaaacat ggtcagtgtt taaattttca tgaaagttgc ttccctcaaa tacaacccca
     1441 atttgctctt taatttcttt ttcatttttt ttactatcaa gaccaaagat ttctatattt
     1501 ccactatcat aagatattaa atttaaaatt gcttttaatg tagttgtttt tcctgcacca
     1561 ttttcaccta caaatcccat tacactccct tttggtactg aaaaagatat atcttgtagg
     1621 ctaaaatctt tataagtttt atttaaattt tttatagtta ttgcatccat acttatcatt
     1681 ctcctttata taataaaaat aaaatatctt ttaattcgtc atatgtaata tcactacttt
     1741 ttgctgtatc aactgctttt tgtaggtatt cttcaattaa acgaaattgt tcttctttga
     1801 caaaatccat gtttttacta gatacaaaac tgcctttacc agtaactgtc tcaataaatc
     1861 cgtctcgttc taaatcgcta tatgctcgtt ttgtagtaat tacacttatt ctaagttctt
     1921 ttgccaatag tctcatactt ggaagagctg agccttctgg taactctcca cttataatca
     1981 tacttttaat ttgtgtagta atctgttcat atattggctt cccactcgaa ttactaataa
     2041 ctatattcaa taagacacct ccttaaattg tatatatagt atatacacat tatataaata
     2101 tatatataat atgtcaactg attttagaaa aaactaattg ccaatattat acatttaata
     2161 aatgtaatgt attttatgaa agtctgccat attaaataga tttaaatttt tttaaaaaat
     2221 ttataaatat tgttcaaaaa tcctgccaat aaattggaaa tactaaatta aaaaaataat
     2281 aaagtttagc ttatagctaa ataaaagttt gcttataagg tgaacttttt gcattttcag
     2341 ttttgacata catatttttt tgtaatatat ttatattatc aattagagat aaggagagtg
     2401 agaagagata tttaatttgg ataagacatt aaaaaaggat ttaaatcagg aatatacaga
     2461 gagcaaaagt ttagttatga ataaaagtaa gtatatgaat gtttgtttta agaattatat
     2521 taaagtagta gaaggaatgt atactaagag ggatttaatt ccattaaaat taataaactc
     2581 tatggataaa aataataaga ttagaaaaac attatatgaa ttatcttgtg aatttgaata
     2641 tccaaaaact tctttatctt cattgtttac ggagctgaaa aaaatggatt ttttacaaag
     2701 agtgaaaaat ggtgagtata tgataaatcc acatatagca tataaaggta gtagagctga
     2761 taaagaaagt ttattagcag aatataatca gataaaaaga cctaataagg ttagtaaata
     2821 aggaagaagg tttttatatg agtttaaaac ttgatagaaa tgttctacag tggtttgact
     2881 atgtatttga gaatgaaaaa actagtttga gacactataa ttttaattgt acactaaagg
     2941 agattagttc taccagtttg aacaaagtag cttttatttt agaaaaaaat aactctaagt
     3001 actggaagct ttattttgaa ataccagctg aagttacttt aaaacttaag caaaacattc
     3061 atcctttatt tagggaatat atatacgaac aaatatcact gtataacaat aatcaaattt
     3121 acaattttgt aaattcaaac atattaaaag tgtttaataa tattgctatt tatcaatata
     3181 atattttaga aaatctatac actattgatt ttaaaaagag ttttatagat aaatgtcagt
     3241 atttattaat aggtgaaaaa agacttatag atgaagactt gtatttaata gcaaaatcaa
     3301 aagaagtatt cgattttttt aattcagatg gaacatttaa tttaacattg agtttcgata
     3361 tacaaaaaaa cgagaattta cttgatagtc ttttagagct tagaaaatca ataattataa
     3421 atgagagaat ttaatttatt tggatataat attaaaatgg agggatttat atggacgtag
     3481 aaaaaatgac tttaagagtt caaaaaagtt taaatgaggc ttataacgaa gcagtaaaaa
     3541 atcataacca acaagtagat gtaattcatc ttttctcagc acttattaat caagaggatg
     3601 gattgatacc aaatataatc gaaaaaatga atatatcaat tgattctttg agaaatactg
     3661 taaactttga aattgataag cttccaaaag tatatggaga aggtgctgac tcacaaggag
     3721 tatcagctac tagaaaaata aatgaggtgc ttataaaggc agagagtatt tctaaagaat
     3781 ttaaagattc atatataagt gtagagcatg ttatgcttgc aatgatggaa acagaatcaa
     3841 aatcagctgt tggtaaaata ttaaaacaat ataatataaa taagaatgac tttttaaata
     3901 ttttatcaca agtgagaggt agtcaaagag tagaaacaca agacccagaa ggcacatatg
     3961 atgcacttgc tagatatgga acaaatttag tagatttagc caagaaaaat aaattagacc
     4021 ctgtaattgg gagagatgaa gaaataagaa ggattataag aattttatca agaagaacaa
     4081 aaaataatcc tgtattgatt ggtgagccag gtgtaggtaa aactgcaatt gtagaagggt
     4141 tagcagagag aatagtaaga ggagatgtcc cagaaggttt aaaagataag attgtattct
     4201 cacttgatat gggagcttta attgctggtg caaaatatag aggtgaattt gaagaaagat
     4261 taaaggcagt tttaaaagaa gttcaaagtt ctgatggaaa aattatactg tttatagatg
     4321 agattcatac tattgttgga gctggtaaaa cagaaggttc aatggatgca ggtaatctta
     4381 ttaaacctat gcttgcaaga ggtgaattga actgtattgg tgcaactact tttgatgaat
     4441 atagaaagta tatagaaaaa gataaggctc ttgaaagacg ttttcagcct gtaattgcag
     4501 aagaacctac tgttgaagat acaatatcta tacttagagg tttaaaagaa agatttgaga
     4561 tacatcatgg agtaagaata catgataatg ctattgtagc agcagcaaaa ctttctcata
     4621 gatatataca agatagattt ttaccagata aagcaattga tttaattgat gaagctggag
     4681 ctatgattcg tagtgaaata gattcattgc caacagaatt agatgttgtt agaagaaagt
     4741 tatttacatt agaaactgaa agagaagcat tacttaaaga aaatgatgat aaaagtaaat
     4801 caagattgga tgatattcaa aaagaaatag cagagttaaa atctaaaaat gatgaaatga
     4861 cagcaaaata tgaaaaagaa aagagtcaaa tattagatat taaaaactta aaggctcaat
     4921 tagacgaagc taaaggtaaa gctgaaaagt atgaaagaga atatgatttc aacaaagcag
     4981 cagaggttaa atatggtgaa atacctaagt tagaagaaca aataaagcag tacgaagaaa
     5041 atatgaatga tggttctgaa aattctcttt taaaagaaga agttactgaa gaagaaatat
     5101 ctagcattgt atcaaagtgg acaggtatac cagtaactaa actagttgaa ggagaaagag
     5161 aaaaattact taaactagaa gatgaacttc ataaaagagt tattggacaa gatgaagcag
     5221 ttacagcagt ttcaaatgca gtaatacgtg caagagcagg attaaaagat gaaagaaaac
     5281 caattggttc atttattttc ttaggcccta caggtgttgg taaaactgaa cttgcaaaaa
     5341 ctcttgctag aaatttattc gatagtgaag ataatataat aagaatagat atgtctgaat
     5401 acatggaaaa acatgctgta tcaagactag ttggacctcc tccagggtac gtgggatatg
     5461 aagagggtgg tcaacttaca gaagcagtta gacgtgctcc ttattctgta atactatttg
     5521 atgagataga aaaagcacat gaggatgtat ttaacatgtt cttacagata ttagatgatg
     5581 gtagacttac tgataataaa ggtaagacag tagactttaa aaatactctt ataataatga
     5641 cttctaatat aggaagtaat tatctattag aagcaggagg aaatataaca gagactacta
     5701 acaatttagt aatgaatgaa atgaagcata gatttaaacc tgagttttta aacagagtag
     5761 atgatattat aatgtttaag cctttagacc aagaaaatat caagaaaatt atagatatat
     5821 ttatgaaaga tttaaaaaat agattaaaag aaaaagatat tactattgaa gttacaaatt
     5881 cagcaaaaga tgttatggtg agagaaggat atgaccctgt atatggagca agacctctta
     5941 agagatatat aggaaataca ttagaaacta taattgcaaa gaaacttatt gctggagatg
     6001 tgtataatgg atgtactata gttatagatg gaaaagatga aaatattgaa gttttagtta
     6061 aataataaaa gaatcagttg agcataatat actaaatatt ttgctcagct gatttttatt
     6121 tataaactat aggaaaagaa atattttatc aaattaataa aacattttta agtcaatatg
     6181 tccaaattaa atttaactca aaggaagtat tttagtaaat ttcttataat ataatcataa
     6241 tttgaatata attataaaaa ctattgttca tttttattat atttaaataa ataaaagcca
     6301 attaatattg cacctattaa gcaaacaagt gtaacaataa tataaacatt tctcattcca
     6361 aatacaaata ttttatcatt attaatcaca taatcattga ccctatatcc agctttaata
     6421 ctcataaaat tatataaaag tgttgtagaa atagtaatac ctataacttg tcctaaattt
     6481 cttacaaggg aatttatgct tcctacaact cctagcttac ttctagaaca gcttgacatt
     6541 atcaaagcat tgttagcagg ttgaaatata gcttgtccta ttgatattat cgatatataa
     6601 attacaaata aaataagggc agaactttct ttcaatcttg acattagaaa gaatccaaaa
     6661 ctcataacga gaagaccaat taaaataatt ttttctgact taattttatc tgatatattc
     6721 ccaaatatag gtgaaaaaat ggctaaaatc aaaggtgata gtatcataaa taaacctgct
     6781 tgaatagggg gaagtttcat agtactttgt aaataaaatg gaattaaaat acttgatgat
     6841 gcaatacaaa tgaatgatgt aagagcacaa attaaattca atgaaaattt aaaatttcta
     6901 aaaatttcaa ggtcaagaag cggttgagga tgtttttttt ctaatatcaa aaataatata
     6961 ataaaaataa ttgataataa tagtgcaatt aatatatatg gatttagtaa tcctgtttgt
     7021 tgagcactaa ttagagcact aaaaaataat gttgttccta aaaattgaag tatagcacca
     7081 gttttatcca tattttctga agatttttta ctattaggaa gaaattttgt atttaaaaag
     7141 attacaataa gaccgattgg aatatttata aaaaatataa cattccaatc aaatattgac
     7201 aaaataaatc ctcccacaga tggtccaacc atatttccaa tagcaactgc agtaactaaa
     7261 atccctaggg cttttcctct gctttctttt ggaaatagtt ctgtgataat tccatgattg
     7321 tttgccatgt aggcagaagc gcctattcct tgaataaagc ggcaaactat aagtaaagtt
     7381 aaagaattac ttaatccaca tagtaatgaa gctgatgtaa agagaatagt acctacttga
     7441 aatactcttg atttaccaat aatatctcca agtcttccaa aaaataatag tgttgaacaa
     7501 ataataattg agtaacttgc tattacccat tcaatagatg ctagagatac atttagtttg
     7561 cttgcaagaa ctgggagagc aacatttata atactagaat ctaatgttgc cataaaagtc
     7621 atggcaatga ctacaaataa aactgtgatt ttttttctat tatcatcttt tttaaaagag
     7681 tttgtcattt ttattacctc cttattataa tgactgcatt atgttaatgt ttacaatgat
     7741 agttacattt tatataatag cgatatgaaa taatgaatac aataactata ttgcttataa
     7801 ttagcactat tttgatgagg ggggaataat gaaggataaa aatacatatt ttaacaggaa
     7861 aggtcaagaa aactttatag atattttttt aaaggatgac attaaaaaag gagcaaactt
     7921 taaaagacat tggcatgagc atctacaaat atattatttt attgatggta tagcatatct
     7981 tgaatgtgga ttggataatt ttaaggtagt gcctggaaat attgtaatag caaacagtaa
     8041 tgaattacat tatttagaaa gcttatcaga taatttaaaa ttctatagta ttcgtattga
     8101 acccacattc ctttttagta atcaagttga tttgttacaa accaagtact tagcaccatt
     8161 ggctttaaat catattgtat ttaataattt aattcaaaat gattctctga ttttaaaatg
     8221 tattacaact atacttgatg aatatcacaa taaagaagtt ggatatgagt tatctataaa
     8281 agctaatatc tatcaattaa tagtactctt attaagaagt catattgata aaatatattc
     8341 tgaagatgaa tttataaaaa gaaagtattc tttagaaaaa tttgaatgta tctttgattt
     8401 gatagaagaa aaatatatgg ataaaattac attaaaagaa cttgctagtc atgtgaactt
     8461 gagtgcacat cacttttgta gaacatttaa gcaaataaca ggtaagacta caatcgattt
     8521 tataaatgga atcaggcttg ataaagctat atattattta aaggaaacag atttaaatat
     8581 gacagaaatt gctatgagat gtggctttga tagcataaat tattttagtc gcttatttaa
     8641 aaggaattat aatatatctc caactaagta tagaaatgaa cataaagaag ttagtaatgt
     8701 tattttaaaa gaagatatga aataattcat aaatatactt tatagaaaat atatattttt
     8761 aaatctttga gtttttctta ataaatatta ataaagtata gtacataaat gagtaagact
     8821 acttgtatag ttaaatgtga aaatgagtat gaaagaatgt agtgtctatg aaaagtatat
     8881 tttaatatga aactaaaatt ggaaaaataa gaatagcaga aaaaatgatt atattataaa
     8941 tcagtttttt tgagacatct ggcttagatt ctgaaagata tttaatcaaa gaaataaaac
     9001 tattaaataa gcaagtaaac agctaaaata gtatgatttt tatagaaaat ttaaatccat
     9061 cctaaagaat taaaagaaag tcaaataaat ataaaaaaat tatatattta taaaaaattt
     9121 tataataaaa atcatcctga aaaatatata aaaatagaaa aaacatacaa gattctacaa
     9181 taatagattt ttttgttatt attacgagtt ttttattgca actgtagaat aaaagtatta
     9241 ttatataatt aagataatta tctagggaaa ttttggataa tcaaaatata ttttataaaa
     9301 tcaaaataca attacataaa ctttattaaa aatttgttgg gaatttctga ctaacattaa
     9361 gaacgtaagt ttgttggggg atggtatatg tggaaaagtc aaacactact gttacaaaaa
     9421 aagaaaaaat tgaagatata ggaatgaaat tagaacattt attgagtgaa gatattactt
     9481 tttatgaatt attacttgag attttgtata aagaaaaaga agagaattaa ttctcttctt
     9541 taaggaatct ttttaaatag gcattatcaa tcattttttc tatagcttct ctatcttctt
     9601 tttcaagaga atatattttt tctaagagct tttttgtttt ttcacttact gataagttct
     9661 ctatgattaa tttatcttct ttgcttattt ttgtattatc atttacgcct aataaataat
     9721 ctgttgatgt ttctaatgct tttgctaatc taactattat ttcactttta ggttctctta
     9781 aattattttc ataacgtgac aagctagcct cagttatacc gactttagta gcaagttctt
     9841 tttgatttat atctaattct tttcgtagat ttgctattct atctcctaaa ctttccatat
     9901 gtacatcact ccttaccgaa attataagtt attttgataa aaagtaaaat aaaattacca
     9961 aaaatataaa aaaaaaaggt taaaataata aaatatattg aaaattacca tttgtatatg
    10021 tataatatga attatacaaa tggtaatttg atgagaggtg gtatgttgaa ataagaaact
    10081 gaattaatta ataaatacat agaaagtttt gaaaaccttt aattgagata taaacaatat
    10141 gtacaaaatt ttttgcaagt aaaattacca aatgtataaa ataaatcagt agcatagata
    10201 attttatgct ttaatacatt agattattta atttagttat aatggtcaaa aatgcaatct
    10261 acaaataaaa caaagtatta tcaaaaaagt attctttaag aaaatttttt attagagatg
    10321 taattgatat aaataatcgt attaaaaaat aaaatactaa gtttaaaaat ttaaataatc
    10381 tataaatcaa ttataaaata ttatttatta gaaattaaaa tgaattaagt aaatggagga
    10441 attgatatga taataattag aagtcaagat aaaactaatt taatgcagat aaaccaaatt
    10501 aaaatagatg gtagtcaagt ttatgcagta tttgaaagta aaattgatac tgtaaaaata
    10561 ggagattatg aaaataatac aagagctata caagtgttgg acaatataca aaacttcatt
    10621 gaaaatggaa caaaatatga ttatataaca tcaaataaag tcagatacaa tgttaataaa
    10681 atatttcaga tgccagctaa atagtgagtg tgcattttaa tttaataact aaagcccact
    10741 ttcttttgta gtatgaaaga gggctttaca tatttgtaaa tatcgaagta cacttatttt
    10801 atcaataaat tatttaataa ggtataatgg accaattaaa caataaaaat tctcctaaaa
    10861 cagcaagtat taaatatata atagcttcat ttttgtaaag agaggtaatt tttttgctta
    10921 tgtcatcatc aattgtaata gcaatctcat cttgaataat atttttatat atagatggga
    10981 aaatatcttt tttctttata agattaaata aattatttac acctacccat ataaaatatc
    11041 ctaaaacagc acctaatgtg ttcatcatta aatcatcaat atctgtagct cttaagttaa
    11101 acaattgaca aagttcaatt gctaatgaaa aacctaatcc agttaatgaa acattgataa
    11161 tatttctaaa atttttccaa attaaaggta gtaaaaatcc taaaggcata aacataatta
    11221 tatttaaaat atatgttaag atgccaactg agtcaaaagg tattagattt atttcatcaa
    11281 ttctaaaaag cccactatac cttcctatat cccatatgct accaattcca gctttactaa
    11341 gtgctagata gatatataat aaaaaaatat atacccatac taaatgtatc attacattct
    11401 tttcaacttt caaatttttt cgttttataa cgactatttg gtatattaag catggtaata
    11461 taacgcaaat aaatgaataa aatgtccata tcattttatt ttgtcccctt tcaaatttat
    11521 gtttttagtt ttataagtac tatctttata ttaaactgta agttttaaaa atcttttaat
    11581 aaaatcttaa aaatttatta atttataaaa atatattatt aagattaaaa aat
//
LOCUS       DAMJYC010000114        11413 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJYC010000114 DAMJYC010000000
VERSION     DAMJYC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.118"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            59..943
                     /locus_tag="PC472_003349"
     CDS             59..943
                     /locus_tag="PC472_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2728651.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1042..2217
                     /locus_tag="PC472_003350"
     CDS             1042..2217
                     /locus_tag="PC472_003350"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2728652.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2345..3199
                     /locus_tag="PC472_003351"
     CDS             2345..3199
                     /locus_tag="PC472_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728653.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3243..4478
                     /locus_tag="PC472_003352"
     CDS             3243..4478
                     /locus_tag="PC472_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2728654.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLSPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4579..5520
                     /locus_tag="PC472_003353"
     CDS             4579..5520
                     /locus_tag="PC472_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2728655.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5739..6092)
                     /locus_tag="PC472_003354"
     CDS             complement(5739..6092)
                     /locus_tag="PC472_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2728656.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            6388..7299
                     /locus_tag="PC472_003355"
     CDS             6388..7299
                     /locus_tag="PC472_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2728657.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7559..7993
                     /locus_tag="PC472_003356"
     CDS             7559..7993
                     /locus_tag="PC472_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728658.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8016..9551
                     /locus_tag="PC472_003357"
     CDS             8016..9551
                     /locus_tag="PC472_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728659.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTLKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9635..11002
                     /locus_tag="PC472_003358"
     CDS             9635..11002
                     /locus_tag="PC472_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2728660.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFIGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11037..11261
                     /locus_tag="PC472_003359"
     CDS             11037..11261
                     /locus_tag="PC472_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2728661.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         4549 a         1053 c         2114 g         3697 t
ORIGIN      
        1 tatataaact aataaaaatt aaataatatt aatcaactta ttgaatggag gaataaaaat
       61 gaaatttgtg actttttgta gtagcaatga agaaaaaata ggtgtattta attcagaaac
      121 aaatagtata tatgaaatta atagtttagg attaagtaaa ttatatactg atatgaatga
      181 ttttattgaa aacgtatcta caggagattt agaaaaaata aaaaacaatt catttgaaaa
      241 cgcaaagtgt tataagttag aagaagttaa attatgttcc ccaattgtaa gacctaaaaa
      301 agatattata tgtttaggtc taaactataa agaccatgtc aatgaaatcc cagatggagt
      361 tataaaaaac gtagttatgc cagattatcc aatttatttt tctaagagag cagacaaaat
      421 aattggagtg gatgataaga taagcttaca tggagatttg gtagagaagt tggactatga
      481 atcagaactt gcagtaatca taggtaaaga aggtataaat atatcaaaag aagatgctta
      541 tgaatatata tttggatata caatagtaaa tgatataagt gaaagagcac ttcaagataa
      601 acatgttcaa tggtttagag gaaaaagctt agacacacat acatctatgg gaccttgtat
      661 agtacataaa gaagaatttg aacatccttt aaaattagat ataagtagtg tagtaaatgg
      721 agaagtaaga caagattcta atacagagta ctttatattt gatataccta ctataataaa
      781 tgatttatca agaggaatga ctctaaagcc aggagatata atttcaactg gaactccagc
      841 aggggttgct atggggatga atcctcaagt atatttaaag catggcgata tagttgaatg
      901 taaagtcgaa ggaataggag ttttaaaaaa tatagttgat taatggataa atatgccata
      961 taattttatt agtacgaata atttaattaa tataggatta atcaaactta tgattaattt
     1021 attatagaga gggatgagta tatggcaaga gaactagaat taaagtatgg ctgtaatcca
     1081 aatcaaaaac catcaaagat ttatatgaag aatggagaac ttcctataga ggtattaaat
     1141 ggaaaacctg gatacataaa ttttttagat gcatttaata gttggcaact tgtgaaggaa
     1201 ttaaaagaag ctacaggtct tccagcagca acttcattta aacatgtaag tccagcagga
     1261 gcagcagtag gggtaccttt atcagatact ttaaagcaaa tatattttgt tgatgattta
     1321 gagctttctc cattagcttg tgcttatgca atggcaagag gagcagatag aatgtcttct
     1381 tatggtgatt ttatagcact ttcagatgta tgtgataagg agactgcaac tattatagca
     1441 agagaggttt ctgatggtat aatagctcca ggatatacag atgaagccct tgaaatatta
     1501 aaaggaaagc gtaaaggtaa ctataatatt gtaaaaatag atgaaaatta tactcctgaa
     1561 cctattgaaa caaaggatgt ttatggaatt acttttgaac aaggtagaaa tgaaatatta
     1621 ataaatgaag acctattaaa agatattcca actgataata aaatttttac agatagtgca
     1681 aaacgtgatt taattatagc tcttattaca ttaaaatata cacaatcaaa ttctgtttgt
     1741 tatgctaaag atggacaagt aataggggta ggtgcaggtc aacaatcacg tattcattgt
     1801 actagacttg caggtaacaa agctgatact tggtatcttc gtcaacatcc taaagtatta
     1861 aacttaaaat ttaaaaagga tataggtcgt ccagatagag ataacactat tgatgtatat
     1921 ctttctgatg attatatgga tgtgcttgct gatggcatat ggcaaaactt ttttgaagaa
     1981 aaaccagagc ctctaactgg agaaggaaaa agagcatggc ttaaaacttt aacaggagta
     2041 gcacttggtt cagatgcatt tttcccattt ggagataata tagaacgtgc aaaaagaagt
     2101 ggggtaagtt ttatagcaca accaggtggt tctattcgtg atgataatgt tatattaact
     2161 tgtaataagt ataacatagt catggcattt actaaaaata gattattcca tcattaaaat
     2221 actgaaagta taaagataat atagattaag taaaaatcta caacaagttt tgttgtagat
     2281 ttttttattt gctgtataat tttaaataga actttatttt tagatattca atttggaggt
     2341 tgatatggct ttaaatataa gaaactatag cctattatgt aatcaaggaa cagctaaatt
     2401 taatgaagat gtagtcggta taaatccatt tggggcatgg gttttagatg gtgctacagg
     2461 acttaatgga aaaaatttag tgtcaactga aagtgacgca aggtggtatg ttcagtggtg
     2521 gaataggtat ttatataaaa atataaataa agaagaatca ttaaagagta ttattaatac
     2581 tggaataata gaagtaaaaa aagagtatga actaagatta aatgggttta aggttgaaaa
     2641 attagattta ccatctagtt ctatagttgt tgtaaaattt catgaaaata aagtagagta
     2701 ttttttatta ggagattgtg ctttgtttat taaaaatgga gattccaaag ttataaaaga
     2761 taggagtatt tgtaaatttg ataatactgt atttgatgaa atgagtaaac tttctaactt
     2821 ggaagagatg gcttttgatg agattagaac tagtgttatg gatacaatag taagcaatag
     2881 attgaagaaa aatacaaaag agggctattg gatattggat tttgatgagg aagctgtgga
     2941 aaattctata tatggataca ttgaaataaa aaaagatttt caattgatgc taacgagtga
     3001 tggttttaca agtgcatgtg atagatataa tctaataaaa gaagaagaat tgatacgtat
     3061 agcagaaaaa tttggtatag gatatataca taataaagta agggattttg aagataatga
     3121 ctataaagcg gttaaagtgc caagatttaa agtaaaagat gattcatctt gtttatattt
     3181 agatatatat aatgattaga ctgtataaaa ctatttaatt ttagaaagga ggcaagtgaa
     3241 aagtgaaaat tcttcatata ataacacaaa aaccaaatag tacaggtagt ggtatatatt
     3301 tgtctggtat gataaaaggg ttcgaaaaaa tcggtcataa gcaagctgta attgcaggta
     3361 tagatgtaaa tgatgatgta aattgttttc caagtgaagt aagtttttat ccagtaaagt
     3421 ataattgtgg ggagttaaat tttcctgttg taggtatgag tgattctatg ccatatgaaa
     3481 gtacaagata taaagattta aatatagaca tgataaatag attgaaatat caatttaaag
     3541 taaatataga taaggctatg aatgatttta aaccagaact tataatatgt catcatttgt
     3601 acctcttaac tgcatttgtt agggaaatgg taaaagatat aaaggttatg tctatttgtc
     3661 atggaacttg tttaagacag ttaaatacca ttgatttaga gaaagaatac ataatagcaa
     3721 atataaggaa attagattta atatttgctc ttcatgaaaa tcagaaatat gatattataa
     3781 agacttttgg agtaagtgaa agtaaagtag ttgtaattgg tagtggttat aatgatgata
     3841 tattttataa taaaaattat aaaataaagg atgataaaat aaaaatagta tttgcaggta
     3901 agatatgtaa atcaaaggga ctgagtccat ttataaaagc tatatctaaa ttaaaatata
     3961 gtaaagattt aatagaagtc aattttgcag gtactggaag cgatatcgaa tcatataatg
     4021 agatagttaa actggcaagt aagtctccat ttaaaatgaa tttcctagga aagttagaac
     4081 agagagattt agctgagcta ttcaacaggt cacaaatatt tgtgttacca tcattttatg
     4141 agggattacc agttgtagtt ttagaagctt tatcttgtgg cacagatgta atcactactg
     4201 atattcttgg tgttaaagag tggattggta gtgagattaa taattctgga aagatagaat
     4261 atgtttctct accttttatg gagaaagaag gtattccaaa agatgaagag ttatatgatt
     4321 ttgaaaataa tttgtataat gcaatagatt ctaagataca atctttattg aataactcaa
     4381 ataagaaaac atctgtagat atgtcaaaaa aaacgtggga tggcttagca tatagaataa
     4441 atgaagttat tttaatggat gaattgtgtt tagtctaaaa ttatttaaat tataaaaaag
     4501 tgtaactatt tttacactat ttttaaatga ttttatatta tactaatact aaatatattt
     4561 gttttaggga ggtaatttat gaggattagt aaaaaggtat tggcgttagg tatttcagca
     4621 ttactgttaa gtgttagttt tccaacatca ataaacgcac tagataagat tgaaaatatt
     4681 caaggagtag ataaatatga aacagctggg cttatttcag ataagcaaga ttttacaaca
     4741 gcagttctta tcaatgcaga ctctacaatg gtagatggga ttgctgcaag tggtcttgca
     4801 ggagtaaata atgcagctat tttattaact aataaggatg atataccaga agcaacactt
     4861 caaagactga atagagttac taagatatat gtaataggtg gagaaaattc tataagtaaa
     4921 gatgtagaaa aaatgttact tatgagaaga atgcaagtta taagaattga tggtgtagat
     4981 agagtagata caagttataa aattgcaggt gaaatagaaa aaattaaaaa cagtgataag
     5041 atgtttttag taaatggatt taaggatgaa gctgatgcag taagtgttgc atctgttgct
     5101 tatagagatg gagctcctat aatattgaca aaagatatac cttcagcaga agaagacaca
     5161 gacttagacc catggtttgg agtttctcct gttccagtat atgctattgg aggagagtct
     5221 acattaagtg attacatagt gagcagatat agagcaacta gaattggtgg tgttgataga
     5281 tatcaaacta ataaaaatgt tattgaaaag ttctataatg gagcaaaaga gttctatata
     5341 acaagtggag atgatttagt ttatgcttta gttgcatcac cacttgcaaa aaatgctcct
     5401 gttgttttag tttcaaataa aagtgataag agtatattaa gtggagctag taaagtaaca
     5461 gcaataggta taagtgataa gagtattata gagcaatgtt tggatgctgt aaagaagtaa
     5521 tggagtgtta ttagtaaaat gaatatatac attgaaaatt agtagattgt tcattttata
     5581 atggtataaa atatatggga agaggctatc aaatggagat aagcctcttt ttcattgtta
     5641 aaattatgaa aagatacttt attttatata aaaaagtatc ttatgaacta aatgtctatt
     5701 ttgagacact atctactttt cttatattat attatttttt atatggaata aatttcataa
     5761 gtattatctt cctttaattt ttctaaccaa accatctcta aaatatctag ttcttctaaa
     5821 tagtctttct ctgttttgtt atcatcatgt tttataagtt ctaaaacttc aaatgtaaat
     5881 gctttttcac catatttttt ccattcatta tttaattctt ttatcataca tgagccagca
     5941 ccaagctgaa atttgagaga gttaaatttg ctttttatgt cattactttt tccaaggtaa
     6001 atctttttag taatatcatt ttttattatg aatacacctg tgtcaaattt catttctttg
     6061 tataattgtt ttaattcacg ttttctatcc atgattttag atacctctct ttataattga
     6121 tatgtaatat gtaagtgtaa aaatatatta catatcaatt ataaagagat taattttata
     6181 tgtaaatagt ttttataaat ttaattatga attagtcatc aataaacaag aaatttagct
     6241 gtaaaaatcc attataaagg ctattataaa ttttaatatg ctaaaaatat aaaaagaata
     6301 gttgacagtg tagaaaatat ggaataaact aaaagtgtaa tagtaatatg aagttatata
     6361 ttacattgaa aggtgtgaat gattattatg aataaaaaaa tagaattaat agggaatttt
     6421 gcaagtgatt ggattaggta tagtgattat gaatataaaa ttactgaaga agatgaatta
     6481 tatatagttc caacagaaga tgctgtgttt accatgtata acccatttga tgtagcagat
     6541 gatataatag tagatataat tcgcatagga caagaggcat tacaagatat atcaaaagag
     6601 acaaaagaaa tgaaactaaa gaaagaagta cttgaatttg taaaaaagta tggattattg
     6661 ggtttaatat attcaagtgt atataataga gatatagttg gagaagaaag agtactgatg
     6721 attgaaaaga attatattac aaaagagaat atattgagta cagatgaata tgtaaacatg
     6781 tttataccat ttgcaagtga tgaagatgtg tattttaaaa agtatagaag gggtatagat
     6841 gtatcaaaga gagaagaatc tccaaagttt tttggaaaga gaccattgat tttagattta
     6901 gtattttcaa agttctacac tgaacaacta gattggattg taagttttgc taaagatatg
     6961 tgtaagcatt ttaatcaaat ttgtgtatat aaaaatacat ctaatctatt aactgaaagt
     7021 gttactatta tgtctggagg gtttaatcct caaaagatag gattttctat aagccaatta
     7081 gataaaccaa ttattgcatg ggagtttgat tctttgaaaa cagctattga gactatctat
     7141 gcctttgcaa ttacaagtga accttcttct ttaaataaat gcaagtactg tggtaaaatt
     7201 tatattccta caaattctaa atctcaatac tgttcacctt cttgtagaaa ttgctcaaat
     7261 gtaaagaaaa gtagaagtag agccaaaaaa gataaataaa attaataaat atatcattgt
     7321 ttagatttta gtaaataact actgaattaa aagttgttat agaaaattat atatctaaat
     7381 gatggtattt ttttgttgag aaaattgaaa agtttaattg agatagtaat tttgagttga
     7441 caaaaaccac ttttaacact actattataa gtggttggta aaataatata cattaattta
     7501 agtatagcat aaaaaacaaa aaaagtcaat ggaaaaaata aaaaaggaga atttaaaaat
     7561 gatatcacct attattttga gttctataaa tcagaattta aaggaaatag aaagaaatga
     7621 actgcttgaa actaatatag aatctggaga ttatggtcta gctctttctg aatctgatgt
     7681 aaaagatata ataaattcga gagataatac tttaaagggg tatggaagaa tagaattaga
     7741 tataaaagtt acaaaacagt taatagaaaa tatatataca tcacaatata caaatgtgga
     7801 caattattta gaggctatta atgatatgca agaaattttt tattatttaa aaaatgaaac
     7861 tgatgacaag atatgtgatg atgagattat agaaatactg ggtgaatttt atgaaaaatt
     7921 ttcaggcaat atggacaatg taagaggaga agcagacgaa tttgcaaaga aatttaaatt
     7981 tggagaagtt taatatatga taggagagat tttgtatgtt aaataattta agtaatatta
     8041 atataaacga aaataattta ataaagaacc aatattccat atctctactt aaggaatgtc
     8101 taaattgtaa agtgatagat gaaagagaag tatataatat acaacaagaa gtatctctaa
     8161 tacttatgga cttgattaaa aaatatacaa atggtcaaag cacatcagta aaaacagaag
     8221 ttgccgaaaa actgttgatt tcaatatggt atgctattga tgcatatata aataagcttg
     8281 gagatataga aaaaagagtt gtggagttga ctgctaagaa tgttaaaaaa gtgtaccttg
     8341 gaggcattga aatattagag gaagagttag ttaggctaaa agatttttat aaactaatga
     8401 tgagtagaaa attggatact aattcaatag catataatga taccttgatg gagttatcaa
     8461 gtttctttaa aagttatatt gtaaaatttg aagctcagga cgttccagta agtatagatt
     8521 atccattggc actagatgat atggatgtta agggtattta ttatgtgaag aattatattg
     8581 agagtataga tatagaaaat agattttgta acctatttaa gaaaaaagat agagaaaaat
     8641 tattttatga ctatgggatg acttataaaa tagattgtga tgttatactt gtaaatttat
     8701 ttgaacttat tatgaataat tgtatttttt ctactatttt aggaaatgaa gtgactaatt
     8761 tagaaatttc aaaatatgag tatgaatttt tagaaaaaca atttaaaaat atgtacaata
     8821 atattactga agataaagtt atagactctg aagaaagtaa tataattgaa ttgattgaag
     8881 tagatgatag ttttaacact gaagtagata gtaatttgga ttataatgat tttgaagaaa
     8941 ttaacagaga aattagaata aataaaaaaa taactttact acttatggaa gcaattgaag
     9001 tattaataaa aagattaagc atagaagatg aagatgtaat aagatatatt aaaaaatatg
     9061 aaaagatatt tattgaatct gtgctaagct caataaaaag taacacttta aaaagtatgg
     9121 taactgtgag taaaaataaa aatgaacact tagaagatta tattgttgat gatgaaagta
     9181 aattggatga tgaatcgttt agagagatat tcaatgagat attagatagt acatctataa
     9241 ttgaaaaaat aagaataata aaggaaaata tcaatgctaa aaaagacttt atcgatattc
     9301 ttgaatctga atgtttattt ggagaagagt atttaatgtt gtttgcatca ttaagtgaat
     9361 tagaattggc aatattgggt agtgtagtat tttatgaaga tattagaatg agagaaatta
     9421 atatattaga atttatgtta aatgagaaaa ctgaaactac tttatggaag atagagtaca
     9481 ttaaatttat gaagaggcaa agtgaagata agatatactc catagaagag catatgaata
     9541 aaattaatta acaaaaaatt gtgaataaat agaatgttag tatataatat aatctgtaaa
     9601 gcatatcaaa aatacatata tttggaggaa aattatgaaa gagtatatca taaataatgt
     9661 tgaagaaata agagaagaat taattgattt atctaagaaa atatgggaaa atccagaatt
     9721 agcctttgaa gaaaagtatg caagtagcat tcaaaaagaa tacctaaagt caaaaggatt
     9781 taaaattgaa gaagtagaaa atttacctac tggatttata gcaagttttg gagagggtaa
     9841 acctgtgatt ggcatacttg gagaatatga tgctttacca gagctatcac aatgtgtaag
     9901 tgcagaaaga aagccattgg tagaaggaaa agctggacat ggatgtgggc ataacctgtt
     9961 gggtgtagca ggtgtaggag cagttgtttc tataaaaaaa ttaatagaag aaaataaatt
    10021 taatggaaca ataaaatatt ttggttgtcc tgctgaagaa gaaggtggag gaaagacagt
    10081 tatgtgtata aatggctgtt ttgatgatgt agattgtgca tttacatggc atccttttga
    10141 tataaatgca ccttggagag gtggaagtct ggcaaactta tctgtaaaat ttaaatttaa
    10201 agggataaca gcccatgcag ctcaagctcc tcataatggt agaagcgcac ttgatgctgt
    10261 tgaaattatg aatgttggag ctaattattt gagagaacat gttattgact ctataagaat
    10321 gcactatgtt ataactaatg gtggtggaag acctaatgta gttcctggat ttgctgaaag
    10381 ttggtacttt attagaggta aaaaagcaaa agatgcagaa cacgttcttg atagattaat
    10441 aaaagtagct caaggagcag caatgatgac agaaactgaa atggagtata aggttacaga
    10501 tggaatatat gactatatac caaaccaatg tttgactgat ttagtttatg acaatatggt
    10561 ttttattggt tgccctaaaa atactcctga agaagaagaa tttgcaaaaa agttgtgtga
    10621 tactcttact agagaagaaa gattaggtgt agcaacatca ttacaaaatg ataaatctat
    10681 tgtagaaagc tatatacaca aggaaatagt tgacggtgac aaagataaag gactagctgg
    10741 ttctactgat gttggagatg taagttatgt aataccagtg gctcaatttg caatggcagc
    10801 ttggcctgtt ggaattgcaa gtcatacatg gcagtcatgt tcatcagcag gttcaaatat
    10861 aggattttct gctatgataa attcagctaa agtactggct tgtagtgctt atgatgtatt
    10921 tatggatact aaaattattg atgaagcaaa aattgaattt gataaatcat tagatggaca
    10981 aaaatttaaa ccattggtat aattttggta ataaaatatt tcagggggga atagctatga
    11041 gagaaaaagt tgaaaaagtg cttgaggaaa agataaaacc agtattacaa agagatggtg
    11101 gagatgtaga gcttatagat gtaaatgaaa atggtgtggt tttagttaga ctacaagggg
    11161 catgtagtgg gtgtccagga gcgactatga caatcaaagc tattattgaa aatgttttgg
    11221 tgagtgaagt tccaggagta actcaagtat taggtgttta atagttagcg tttattaaat
    11281 gtatgttata cataagtatt taataaagaa gtaaaaaaaa tagtgaaaca atatatatga
    11341 aaaagtatga gaaagataat aaaacattat agaaataatg aaatatttca tatgtatatg
    11401 aagaaataag aaa
//
LOCUS       DAMJYC010000115        11396 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJYC010000115 DAMJYC010000000
VERSION     DAMJYC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.119"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            166..303
                     /locus_tag="PC472_003360"
     CDS             166..303
                     /locus_tag="PC472_003360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728662.1"
                     /translation="MGNSDCSKMLAFYEIMKMLETIDEAKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(331..723)
                     /locus_tag="PC472_003361"
     CDS             complement(331..723)
                     /locus_tag="PC472_003361"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728663.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            911..1105
                     /locus_tag="PC472_003362"
     CDS             911..1105
                     /locus_tag="PC472_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728664.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            1141..1932
                     /locus_tag="PC472_003363"
     CDS             1141..1932
                     /locus_tag="PC472_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF2728665.1"
                     /translation="MNNLQVIERNNKRVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFCLQGDDLRIFKRGSYDIGIAPNVNKLYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQQKLTCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLNA
                     TDWRKDTQQLIARIAKKQGGFEHINMLRRESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            1994..2161
                     /locus_tag="PC472_003364"
     CDS             1994..2161
                     /locus_tag="PC472_003364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728666.1"
                     /translation="MENKKIEEIKNALIGVNQIEWSLVKSFIDMYFSKKAAKLEIDDL
                     DNFDLYIKRKF"
     gene            complement(2158..2397)
                     /locus_tag="PC472_003365"
     CDS             complement(2158..2397)
                     /locus_tag="PC472_003365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728667.1"
                     /translation="MTKIYACLAGNWVNLCDDPECKMGSNHSSPYIWWEEGADVWSPS
                     NKEEEHTMYQQDYVHIYYKGVDYRIHPIFIQIASN"
     gene            2473..2670
                     /locus_tag="PC472_003366"
     CDS             2473..2670
                     /locus_tag="PC472_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728668.1"
                     /translation="MLIGDNISQILRKRNIKPYKLAKELGIDESGLYKIIRNEKKNPT
                     INTLIKIADYLDVTLDELVGR"
     gene            2709..2918
                     /locus_tag="PC472_003367"
     CDS             2709..2918
                     /locus_tag="PC472_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728669.1"
                     /translation="MAKAVAKEPLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            2919..3059
                     /locus_tag="PC472_003368"
     CDS             2919..3059
                     /locus_tag="PC472_003368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728670.1"
                     /translation="MNVRVLIAYIQFCNDKQIKASFEGLRKYNRDIELVNLYLPAHLK
                     IK"
     gene            3091..3231
                     /locus_tag="PC472_003369"
     CDS             3091..3231
                     /locus_tag="PC472_003369"
                     /inference="COORDINATES: protein motif:HMM:NF015807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2728671.1"
                     /translation="MQREQTTIRLPKELKEKLEKQASKKGRSFNSILLSILQEFIQNP
                     NV"
     gene            complement(3209..3397)
                     /locus_tag="PC472_003370"
     CDS             complement(3209..3397)
                     /locus_tag="PC472_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2728672.1"
                     /translation="MAKMGTYKNPHFAIRIPKEKLDKLKYIAEYNARSANKEIEFLVT
                     KHIEMFEKEHGPIHLDSE"
     gene            3553..3711
                     /locus_tag="PC472_003371"
     CDS             3553..3711
                     /locus_tag="PC472_003371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728673.1"
                     /translation="MLANRVRTGLRIPYDLNTKLILIAEERGMSKNSLILQILWDYIK
                     EIENKEVK"
     gene            3711..3938
                     /locus_tag="PC472_003372"
     CDS             3711..3938
                     /locus_tag="PC472_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728674.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            4133..5023
                     /locus_tag="PC472_003373"
     CDS             4133..5023
                     /locus_tag="PC472_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDF2728675.1"
                     /translation="MDWLKNRQLGIGGSDASAVAGLNPWKTSVQVYIEKKEEIPIETK
                     SFRMELGNRLEGLVAELFTEETGLKVRNVNGMLKNEKYPFAIANIDRAIVGEKAFLEC
                     KTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATHCYIAALLGNEKFVWHKINRDNEVI
                     KNLMKIESEFWEENVLKDILPIPDGSDAYSEFLKTRYKNSVKEKIELNLLEDGISKLK
                     RYDDIVLQMKELKGEKQLIEQEIQSEMREFELATLGGRIITWKGATKRSIDTKRLREE
                     MPDIAEKYTNISSYRTFKIK"
     gene            5036..5869
                     /locus_tag="PC472_003374"
     CDS             5036..5869
                     /locus_tag="PC472_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDF2728676.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGHSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEEAINV
                     D"
     gene            5884..6822
                     /locus_tag="PC472_003375"
     CDS             5884..6822
                     /locus_tag="PC472_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728677.1"
                     /translation="MNEDKSVIEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRVHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISKRGLKEEKKKKKPCTKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIVKQPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNHV
                     SKNKFANFEQTFTKYSENELDDIIKKSQKEKFGVGS"
     gene            6842..7240
                     /locus_tag="PC472_003376"
     CDS             6842..7240
                     /locus_tag="PC472_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728678.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCIRKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEV"
     gene            7243..7515
                     /locus_tag="PC472_003377"
     CDS             7243..7515
                     /locus_tag="PC472_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728679.1"
                     /translation="MKHECKKLFLECDKGSFEINDTRIGEVMFESTEIDNPFERVKYE
                     GKATFEIVSGWEYLQREMLWLKILHLSAVVAKIMQYKMLGILIREM"
     gene            7520..7900
                     /locus_tag="PC472_003378"
     CDS             7520..7900
                     /locus_tag="PC472_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728680.1"
                     /translation="MAKIWMDAGTFLEKTIDIEDMFELNLRAIRKRNEKIKNEIEKQI
                     KYEDSEIKTTNRGGPGKIIKIFNINTGEVKILKSAEEASRYIKVSRSYASYLARENKS
                     TEDGWKAEYIQGATDGISKCGTSN"
     gene            7869..8009
                     /locus_tag="PC472_003379"
     CDS             7869..8009
                     /locus_tag="PC472_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728681.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSRYECEGML
                     EK"
     gene            8125..8301
                     /locus_tag="PC472_003380"
     CDS             8125..8301
                     /locus_tag="PC472_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728682.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            8415..9104
                     /locus_tag="PC472_003381"
     CDS             8415..9104
                     /locus_tag="PC472_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728683.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            9122..9343
                     /locus_tag="PC472_003382"
     CDS             9122..9343
                     /locus_tag="PC472_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728684.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEV"
     gene            9340..10344
                     /locus_tag="PC472_003383"
     CDS             9340..10344
                     /locus_tag="PC472_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2728685.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            10426..10755
                     /locus_tag="PC472_003384"
     CDS             10426..10755
                     /locus_tag="PC472_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728686.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            10792..11163
                     /locus_tag="PC472_003385"
     CDS             10792..11163
                     /locus_tag="PC472_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728687.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            11166..11348
                     /locus_tag="PC472_003386"
     CDS             11166..11348
                     /locus_tag="PC472_003386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728688.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
BASE COUNT         4808 a         1083 c         2155 g         3350 t
ORIGIN      
        1 tacatcaagt ctataagtta aggaattttt ataccatttt ttacgaacat aagttctgaa
       61 aagggatata aaatataata ttataaaaag aaggtgattg gctaaatatt ttacatattt
      121 aaggggcaag gaataaatat tataaattat aagagggtga cgcaagtggg aaattcagat
      181 tgttctaaaa tgttggcatt ttatgaaatt atgaaaatgt tagagactat tgatgaagct
      241 aaattttata attgtaaaga agaaataaag tcagagttta agcatataaa aaaagaggat
      301 tgaatcctct ttttttatat atttttatgt ttattcttta tttttagtat tttgctcctc
      361 caaaaaaagt tttgctatct ttaaagcttt atttttagaa tcatcatcaa gttcactaaa
      421 aatatcaagt atttctttca tgtcatcaga tggatttagc tttttaataa gttctttttc
      481 tgtgatatta gaaatattaa ttttgatatt agttctacct aataaatagt cagttgatat
      541 atctaaagta tcagctatat gtctcacaac atcaagcgct gggcttcttt taccagtttc
      601 atatccgtta taagtgtttg ctggtaaact taatttatta gccatctctt tttgcattaa
      661 tccgagttct tctctctctt ctcttaatct tttagaaaat atattgcttt catctttata
      721 cattgtaatt gtccccctct taaaaactat aacgaatgta attactctta ttctactaca
      781 aaatacgcat tttgtgattt taattcgcaa aaaggaagaa aaaaacaaaa aacgtattga
      841 caattcacaa aacggagtat attatataaa tatagaaact tcaaattgag aataaaagga
      901 gtgatatata ttgaaaaatt atatattgaa atctcttaga gtaagacaag gattgagaca
      961 aagagattta gcaaaaatat taggaatgaa tcccagtact tattcaagta aagaaaatgg
     1021 cgataggcga ttcacagtaa gtgaagcaat aaaaatatca gattttttta atacagattt
     1081 gagagatatt tttttaaata aataattcgc gaaatgtgaa taaaaaggag gtagataaac
     1141 atgaataact tacaagtaat agaaagaaac aacaaaagag ttttaactac acaacaacta
     1201 gcagatgtat atgaaacaga tgcaagaaat ataagcaaca attttaacaa taacaaagat
     1261 agatttattg aaggtaaaca ttatttttgt ctgcaaggtg atgatttaag gatatttaaa
     1321 cgcggttcat atgatatagg aattgcacct aatgtaaaca aactttacct ttggactgaa
     1381 agaggagcaa acagacactg taaaatatta gatactgata aagcttggga acagtttgat
     1441 aacttagaag aaacatattt caaagttaag caacagaagc taacttgtat agaagatgta
     1501 ttaatagaaa gtttaaaaga aatgaaagat ttaagacttc aagttaacca agctaacaat
     1561 atagcattag aagcaaaaac agaggttaaa acaataaaag aagttgtttc attaaatgct
     1621 actgactgga gaaaagatac gcaacaatta attgcaagga tagcaaagaa acaaggtgga
     1681 tttgaacata taaacatgct taggagagaa agctacgaat tactaaataa gcgatttgga
     1741 gttgacctac atagaagatt aatcaataaa agaagaaaga tggcagaaga aggtgtatct
     1801 gaatctaaaa gagagaaagt taacaattta gatgtaatac aagatgataa gaaactcata
     1861 gaagggtatg tggctattgt aaaagatatg gctttaaaat atggaatatc aagtgattta
     1921 agcaaaaatt aagttaaatc acaacagcac tttgaaaact aaatacagaa tattttgaaa
     1981 aggagtggtt aaattggaaa ataaaaaaat agaagaaata aaaaatgcat taataggggt
     2041 gaatcagata gagtggtctt tagtaaaatc ttttattgat atgtatttta gtaaaaaagc
     2101 cgccaaactt gaaattgacg acctagataa ttttgatttg tatataaaac gaaaatttta
     2161 attactagca atttgtataa agattggatg gattctatag tcaacaccct tatagtaaat
     2221 atgaacataa tcttgctgat acatagtatg ttcttcttct ttattagaag gactccatac
     2281 atcagcacct tcttcccacc atatgtaagg cgaagaatgg ttactaccca ttttacattc
     2341 tgggtcatca catagattaa cccaattacc agctaaacaa gcatatattt tagtcataat
     2401 atcaccaact ttcatataaa tttggaatat attccatatt tatattatac catgtagaac
     2461 gaaggtgatt aagtgttaat aggcgacaat ataagtcaaa tactaagaaa aagaaatata
     2521 aaaccttata aattagcaaa agaattagga atagatgaaa gtggattata caaaattata
     2581 agaaatgaaa agaaaaatcc aaccataaac actctaataa aaatagctga ttatttagat
     2641 gttacattag acgaattagt tggaagatag aaattaaata cagaatattt tgaaagtagg
     2701 tgtattgtat ggcaaaagca gtagctaaag aaccattatt ttatagagca aaagatgtag
     2761 ctaaattttt agatatatgc gaagcaactg catacaaaat aattgcagaa ttaaatgaag
     2821 aattagaaaa agaaggtttt aagacttttt ctggcagggt atcagttgct tattttaaag
     2881 aaagatattg ttataagcca agaaaggggg tgatttagtt gaatgtaaga gtattaatag
     2941 cttatataca gttttgtaat gataagcaaa taaaagcaag ttttgaaggt cttagaaaat
     3001 acaacagaga tatagagtta gttaacttgt atttaccagc acatttaaaa ataaagtaag
     3061 ctataaataa ttaaagaaaa aggtgattag atgcaaagag agcagacaac aatacgcctg
     3121 cctaaagaac ttaaagaaaa attagaaaag caggcgagta aaaaaggaag aagttttaat
     3181 agtattttat taagcatatt acaagaattt attcagaatc caaatgtata ggaccatgtt
     3241 ctttttcaaa catttctata tgtttagtga caagaaattc tatttctttg ttagctgaac
     3301 gagcattata ctcagctata tatttaagtt tatcaagttt ttcttttggt attctaattg
     3361 caaaatgagg atttttatat gtacccattt tagccataat atcaactcct ttgtgtagtc
     3421 tatttggcaa caattatatc acaaaaataa atacaaaaaa atatataggc aacatataga
     3481 caacataata ataaagtgtt atactttgta ttaaagatag gcaacacata gataacaata
     3541 taggaggaaa ttatgttggc aaatagagta agaactggat taagaatccc atatgattta
     3601 aatacaaaat taatattaat agcagaagaa agaggaatgt caaaaaattc tttaatatta
     3661 caaatacttt gggattacat aaaagaaata gaaaataagg aggtcaaata atggttgaat
     3721 tggtaaaaga atttgattta caaacaatta aagtaggaaa tgcagtaaaa gtaaattgca
     3781 aaagatttgg ttttgaaatt gattgtatag tagtagtagc aacagaaaaa gaattaaatt
     3841 tagcatactt tgatgaaggt agaggctgta tggagtatca agccttaata acagaagata
     3901 ttcaagatgg tgattatgag attaaaattt tatcttagga ggaaataaaa tggtaggttt
     3961 atttgcaata tgtttagcag gattatttca ataaaaaaag tgctggtcaa agtaaccaac
     4021 acatacaaaa aattcaactt atttaggagg ataccataaa atgaataaaa tttcaagtcg
     4081 tagaaaatat ttagatgctt ttatagtaac tgatactaag aacatagata aaattgattg
     4141 gcttaaaaat agacaattag gaataggggg tagtgatgcg tcagcagtag caggattaaa
     4201 tccctggaaa acttctgttc aagtatatat agaaaagaaa gaagaaatac caatagaaac
     4261 taaaagtttc agaatggaat taggcaatag attagaagga ttagttgcag aactttttac
     4321 agaagaaact ggtcttaagg tccgtaatgt aaatggaatg ttgaaaaatg aaaagtatcc
     4381 ttttgcaata gctaatatag acagagctat agtaggagaa aaagcatttt tagaatgtaa
     4441 gacaacaaat agtttttcta taaaagaatg ggaaaatgga gttccacttc attatgaaat
     4501 acaatgctta cactatatgg ctgtcacagg agctacacat tgttatatag cagcacttct
     4561 tggaaatgaa aagtttgtat ggcacaagat aaatagggat aatgaagtaa ttaaaaatct
     4621 aatgaaaata gagagtgaat tttgggaaga aaatgtatta aaagacattt taccaattcc
     4681 tgatggttca gatgcttata gtgagtttct gaaaacaagg tataaaaact cagtaaaaga
     4741 gaaaatagaa ctaaatctac ttgaagatgg tatatcaaag ttaaaaagat atgatgatat
     4801 agttttacaa atgaaagaac taaaaggaga gaaacagcta atagaacaag aaatacaaag
     4861 tgaaatgaga gagtttgagt tagctacatt aggcggaaga ataataactt ggaaaggagc
     4921 tactaaaagg tccattgata ccaagagatt aagagaagaa atgcctgata tagcagaaaa
     4981 atatacaaat ataagttcat acagaacatt caaaataaaa tagggggtaa tatatatggc
     5041 tagtgaaaaa gcaaaaggag cattagaaaa gaaagtttca ggagcaaata cagtcaaggt
     5101 aagtccaagt aaaggtatgg agcaacttat gaataaaatg gcaagtcaga taaaaaaagc
     5161 tttacctagt atggtttcaa gcgagagatt tcaaagagtt gccctaacag cttttagtaa
     5221 taatccaagg ttacaatcat gtgaacctat gagttttata gcagcaatga tggaatcagc
     5281 tcaattaggt cttgagccta atacgccttt aggtcaagca tatttgatac catatggaaa
     5341 taaagtgcaa ttccaaattg ggtataaagg tcttttagaa ttagcacaaa gaagtggaaa
     5401 gataaaaact atatatgctc ataaaataag agaaaacgat aaatttgaga ttaaatatgg
     5461 gcttcatcaa gacttagttc atgaacctaa attaaatggt gatagagggg aaataattgg
     5521 atattatgca gtatatcatt tggatacagg aggacatagt ttttctttta tgactaaaga
     5581 ggaaattata gaatttgcaa agagtaaaag taaaagttat agtagtggac catggcaaac
     5641 agattttgat tcaatggcta aaaagacagt tataaaacag ttattaaaat atgcaccact
     5701 tagtatagaa ttacaaaaag ctatggtagg tgatgaaaca ataaaatctg aaatagatga
     5761 agatatgagc atggtcgtag atgaaagcga aagtttagaa gttgatttcg aagtaaaaga
     5821 aaatatggat ggtaaagtta gtgtggaaga agctataaat gttgattaag taggtgaggc
     5881 accttgaatg aagataagtc agttatagag aaattaaata tattaagtgg tggatacggt
     5941 cttatgccaa gaataatagc aagagatagg tggttgacag ttggcgctag aatgctgtat
     6001 tcatatctaa ctagttttgc agggaatgat ggaacatgtt ttccatctag ggatttaatt
     6061 tgttatgaac tagatatatc aaaagacaca tttacaaagt acaaaaaaga gctagagatg
     6121 agtggctata taagggttca taagaataaa tccaaacaag gcaagatgca aaacaatata
     6181 tatgaaatag tatttgatag aacttatata gatgaatgta tttctaagag aggtttaaaa
     6241 gaggagaaaa agaagaaaaa gccatgtact aaaaagcaag acacggaacc gtatcctaaa
     6301 aatgtagaca tggaaccatg tcctactttt ccggacacga ctcagccgga cacggaaaat
     6361 atggacacta atagtaacag tattaatagt aacagtatta ataatatgta tatagtaaag
     6421 caacctgtgg ataacttttt aaaagaattt aagaagctgt atgaagaaaa tataggagta
     6481 atatatccag ttacagctga atggttatta gaagtatcta atgaagtaga tataagagta
     6541 tttaaaagag ctatagagat atgtgctgaa aagatgaata tgaatctagc atacttaaaa
     6601 ggtatcctta aaaaatggaa ggatgcaaat attactacat atgagcaact ggaatcatat
     6661 agattacagc aagaaaataa gaaaacaaaa aaagtagtta ataaccatgt gagtaaaaat
     6721 aagtttgcta actttgaaca aacatttact aagtattcag agaatgagct tgatgacatt
     6781 attaagaaaa gccaaaaaga gaagtttgga gtaggaagtt aaaatattgg agggatgaaa
     6841 gatggagtta aaatttagag aatggaataa aaatggtaaa gaaatgtata gttatgatga
     6901 aatggtgtgc tattctaaaa atttgcttag agagtgggtt tatagtggtg tttatttacc
     6961 aacaagcaat gaaaattttg aggttatgat atacacaggt ttgaaggatt gtattagaaa
     7021 agaaatctat gagggtgata ttgtttcata catcttatca tttgaagaat ttataggaga
     7081 ggtaaaattt gaagaaggtt tctttgtaat agataatgaa gtgctaggag aatgcgttgg
     7141 gttatttcat gaaattgcag tggttaaagt tattggcaat atatatgaaa atcctgagat
     7201 gttagaaaag ataaggaaac ctaaagtgtt ggaggtctaa aagtgaaaca tgagtgtaag
     7261 aaacttttct tggaatgtga taagggaagt tttgagataa atgatacaag aattggagaa
     7321 gtaatgttcg agagtacaga aatagacaat ccgtttgaga gagtaaaata tgaaggtaaa
     7381 gctacttttg aaatagtatc tggatgggag tatctacaaa gagaaatgtt gtggcttaag
     7441 atattgcatt tatcagcagt tgtagcaaaa ataatgcaat ataaaatgtt aggtatttta
     7501 ataagggaga tgtaataaaa tggctaaaat ttggatggat gcaggaacgt ttttagaaaa
     7561 aactattgat atagaagata tgtttgaact taatttaaga gcaataagaa agagaaatga
     7621 aaaaataaaa aatgaaattg agaaacaaat aaaatatgaa gattcagaaa taaaaactac
     7681 aaatcgtggt ggaccaggaa aaattataaa aattttcaat ataaatactg gagaggtaaa
     7741 gattcttaag agtgctgaag aagcaagtag atatataaag gttagtcgta gctatgcaag
     7801 ttatttagct agagaaaata aatcaactga ggatggttgg aaagcagagt atattcaagg
     7861 agcgacagat ggtattagca aatgtggaac aagtaattaa gttagctgaa aagatattaa
     7921 ataagaaaaa gtgttctgtt aataaagcta ttgatatagc tataaaaata ttaagtaggt
     7981 atgagtgtga ggggatgtta gaaaaatgaa tatattagct attacaatgc ttataatagg
     8041 aagttttata gctggtagag tttatgagta tagattgaat ctaaaagagt gtgaaaattg
     8101 cgacaataaa agaggtgtat aagaatggat gatagattag aaattataaa tgcttctgta
     8161 aattatatac agatgatatg tgaaagttca aatatagcta ttatagcaga gcgaggaaga
     8221 gttagaatat tagatttaga aactaaagaa aaatatgatt tattaaaaaa taaactcgag
     8281 gaaatgttag aagaaatata agtgaaaata tctaattaag acagtttaga gagttgcaaa
     8341 atgtcttttg gtataaatta ttgttgaagt gttttgtgac tctcaaaaat gaaaatagag
     8401 aggggaataa tataatggat ttaaataaaa ttatgaatga tgcactagta gagttagagg
     8461 aaaatggctt tgtagaggaa gtagttaaaa aacaattggc agaaacaata aaaagagttg
     8521 tgaatgaggt ttttggaaat tggggtgatt ttagtaaaaa aattgaaaaa catctaagtg
     8581 aaaatataaa tataaattta gataaattag atatacaaaa atataatgtg ctcgtagcaa
     8641 ctacagtaaa agaaaagatt gatatgacaa tgaaggtaga agctattgaa cacttaaaaa
     8701 gaaatttaga taacatgctt gtaggtgctg aaaaagaata taaaatgagc gaattgttgg
     8761 aaatattaaa aatggataaa tatgatattg atgaatatga aggctatgaa gattgcataa
     8821 catttattct tgatgaaaat aaatatggtt cgggatggat tgaaattgat aaagaaccaa
     8881 ataaaagtaa atacagatgc gaacactcca tattgttgag aaaagatgga actattgcat
     8941 catatagata cgagaataga gaaatatcat ctaaagatat aatgagtgga tttgataatt
     9001 ttggagattt gctatttaaa atatttgctc atgggtcaaa aataattttg gatttaggaa
     9061 atgatataga agattatgat ttaacaaatg gagaagatta ttaaaatagg ggatggaaca
     9121 aatgtcaaag tatgtattga gatggcaaaa aggattatta ctagatgaac gcaagataaa
     9181 ttattcatgt ggaagtatag aaaatttaaa aaagaaagct gaattattag ctaaagatga
     9241 caagatactg cttgtaacaa tagataaagt tgaagaagtt ataaaagata ctagaagcca
     9301 aaaaatggct gaatattttg aggatgaagg ggtggaagta tgataataca caaatttata
     9361 atacatgttt tagataagaa tagcgacaca ccaatactaa atgattttga gggtagagtc
     9421 agccaagata ttgaagcttt ctttcaaaag aaaataagca aagtatcaag agataatgac
     9481 atcagaacag cagtatttaa tgactatagt aacaatctaa ttaagaagtg ttgtgaacaa
     9541 attatttatg atgaaagttc atttttaaat aactctaaag agattgcagc ttatttattt
     9601 gatgttatga aattgaatgc tacattagaa tcttgcgact tagcaatttg tttatactct
     9661 caaaaagatg aaaagaaagt tgctatatta aagcttgatt acaataattc gtatactcat
     9721 tcaattgagt ttaaagatga taaatttaat atacagatgt ctaaaaatga aattaatata
     9781 caagagacta agacggttaa aattgctgct ttggttggat tgagtggaat gaatgacaaa
     9841 tatcatctta gggttttaga caaggatgca gagaaggaag aagctaattc taagtttgtt
     9901 acagagttct taaatgccac taagataaaa gatgataagt ataagactaa gaagttcaaa
     9961 aatacagctg agaattggat aactaatgct cttagtaatg atataaaaca agcagaggat
    10021 gtaagaagta tattaaatta tactttgaga gaaaagcatg aaattgatat aaatgatttt
    10081 gttgataaaa caattaaaga tgataagtta aaagatagtt ttaaagaaca tatggaagaa
    10141 aaaggtcttg ttgaaggatt tagtatagat aaaaaatggg ttgataaaaa gcttaaaaag
    10201 agaaatataa aaactgacaa tggctttgaa ataaaaggta acttaactga ttttgaggac
    10261 ccaatgaaat atacagtaag acaaaatcaa aatgggtcta tagatatagt tattaagaat
    10321 gtaacatttt atgaggaaaa gtaggtactc atgtgactat tggctagaga aggagaagta
    10381 aataataaga ggatgtaaat ttaaactagt taggaggaat aacttatgaa gattttttta
    10441 ttgactatac tgctaataat tatttgtata ttagcaaatt atgtgaaaaa tcgcatatat
    10501 aaaaaatcta taaataatct aaaatataaa tattctgtag gggaaaagat tatatatcat
    10561 caaataaact gttactataa cagaatggtt ggttgtgaaa ttttagaaaa atgttatagt
    10621 acgaaattta gaaaaagaaa taccccgctt tataaagtaa aagcatatgt aggtgataac
    10681 gatacaacat gggttatacc agagtggaga attgaatgtc ttgctacgac ttatggagaa
    10741 tttcctaaat attaaataat aaaaattggc tggagaagga gattgtaaat tatgtttaac
    10801 atctataaag tgaaaataaa gactaaaaga acattggagc aggtaagaaa tcaaagcgta
    10861 gactttgagt attcagaaaa aggattaaaa aatactctga aatactataa cttgattgat
    10921 gatttaaaag taatagtagt taaatttgga gatgaatatt gtctagctaa ttacaatgaa
    10981 gaagatagaa aaataataat ggaagcacat tatcttttag agcaggatga atatactgga
    11041 tgttatataa atgaatatga acgatttaaa aaagattggg aaaatggtaa ttgtgatggg
    11101 gaagcctgta tggtattttc agatgatgaa attgagataa ttgagaagct aagggagggt
    11161 taaatatgaa taaaagaatt aaaatgaaaa aaagattaat tcataaaaag tgtgatgaaa
    11221 gatgtgtcaa ctatgacttt gtaattagca ataaccttat aacttgtaat gtgtgtatag
    11281 gatgcaaata caaagaaaat atggataaag tatgtgaaga gaactataag aaattaagaa
    11341 gtaaatagaa taaaatagtc aaggtaagtt tgtgaatgaa actagaatgt tatagg
//
LOCUS       DAMJYC010000116        11347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJYC010000116 DAMJYC010000000
VERSION     DAMJYC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.120"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     regulatory      197..240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            341..1309
                     /locus_tag="PC472_003387"
     CDS             341..1309
                     /locus_tag="PC472_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2728689.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPVN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1311..1874
                     /locus_tag="PC472_003388"
     CDS             1311..1874
                     /locus_tag="PC472_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2728690.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1882..2571
                     /locus_tag="PC472_003389"
     CDS             1882..2571
                     /locus_tag="PC472_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2728691.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNYFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKDMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2698..3426
                     /locus_tag="PC472_003390"
     CDS             2698..3426
                     /locus_tag="PC472_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2728692.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3440..4114
                     /locus_tag="PC472_003391"
     CDS             3440..4114
                     /locus_tag="PC472_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2728693.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4140..4976
                     /locus_tag="PC472_003392"
     CDS             4140..4976
                     /locus_tag="PC472_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728694.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4992..5663
                     /locus_tag="PC472_003393"
     CDS             4992..5663
                     /locus_tag="PC472_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728695.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5653..6885
                     /locus_tag="PC472_003394"
     CDS             5653..6885
                     /locus_tag="PC472_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728696.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7035..7352
                     /gene="trxA"
                     /locus_tag="PC472_003395"
     CDS             7035..7352
                     /gene="trxA"
                     /locus_tag="PC472_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2728697.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7369..8247
                     /locus_tag="PC472_003396"
     CDS             7369..8247
                     /locus_tag="PC472_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2728698.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            8517..8885
                     /locus_tag="PC472_003397"
     CDS             8517..8885
                     /locus_tag="PC472_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2728699.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            8888..9259
                     /locus_tag="PC472_003398"
     CDS             8888..9259
                     /locus_tag="PC472_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2728700.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(9403..9858)
                     /locus_tag="PC472_003399"
     CDS             complement(9403..9858)
                     /locus_tag="PC472_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728701.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(9897..11162)
                     /locus_tag="PC472_003400"
     CDS             complement(9897..11162)
                     /locus_tag="PC472_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2728702.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVMVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         4562 a         1188 c         1885 g         3712 t
ORIGIN      
        1 cggtttaaat gcgccacatc ttattaaagt tggttcataa ttctaaataa actttaataa
       61 gatacattaa aatataataa attcttttat gctataaaat ttattttata gctattaaat
      121 cagatgttta aaaatttttt aacagtctat agtttttaag agtctatagt aagtagccta
      181 aaaatatgat tttacatctg tacttgttcg agaactccaa gtataaaaaa ctaaggaatt
      241 atatagtggc agtatataat taataccctt agacacacct ttttgaaagt ggaaaaaaga
      301 tgtgtagttc tcctcgacca caaatcaagg aggataaaaa atggaaaaga gaaaagtaat
      361 aattgattgt gacccaggaa ttgatgattc tttggcaatt cttctggctt taaactcacc
      421 agagctagaa gtaattggaa ttaccacatg ttgtggaaat gttccagtaa atataggtgc
      481 agaaaatgca ctaaaaacac ttcaaatgtg ttcttcacta aatattccag tatatatagg
      541 agaagaagca ccactaaaaa gaaaacttgt aacagctcaa gatacacatg gagaagatgg
      601 tattggagaa aacttttatc aaaaggttgt aggagctaaa gcaaaaaatg gagcagtgga
      661 ttttataata aatactttac ataatcatga aaaagtatca ataatagcac ttgcaccact
      721 tacaaatata gctaaagcac ttattaaaga taagaaagca tttgaaaatc tcgatgagtt
      781 tgtgtctatg ggaggagcat ttaggattca tggaaattgc tctccagtag cagagtttaa
      841 ttattgggta gacccacatg gagcagatta tgtttacaag aatttatcta aaaaaatcca
      901 catggtaggt ttagatgtaa ctagaaaaat tgtacttact cctaatatta ttgagtttat
      961 aaatagactt gataagaaga tggcaaagta tataactgaa ataactagat tttatattga
     1021 tttccattgg gaacaggagg gaataattgg ctgtgtgata aatgaccctc tagcagtagc
     1081 atactttata gatagaagta tatgtaaagg atttgaatca tatgtagaag ttgtagaaga
     1141 tggaatagct atgggtcagt ctatagtgga ttctttcaat ttctataaaa aaaatcctaa
     1201 tgcaattgtt ctaaatgaag ttgatgagaa gaaatttatg tacatgtttt taaagaggct
     1261 ttttaaaggt tatgaagaca ttatagactc tgtggaagga gtgatatagt gtgaaaagaa
     1321 aaatatgtgt aaaaactatt acacttatag cttttggtat ttctataaat ataataggcg
     1381 cttttatagc tatgggatta agattacctg tatacttaga ttcaatagga actataatga
     1441 tagcttcact tttaggacct aaatacgctg ttgttacagg tgtgtttgga agtttaataa
     1501 gtggaattac gtttgatgtg tattctctct attttgcacc agttcaaata tcaaccggat
     1561 tacttgctgg aatagcattt aaaaagaatt ttttaagggg tttaaaaact ccaataggag
     1621 tacttttatt tgccattcca acatctataa taagttcaat aatttcagca tttttatttg
     1681 gaggtatgac ctcttcaggt tcatcatata tagtgcaaat cttgaaagtg ttaggactgg
     1741 gagatgtttt tagtgtgttt gtaacccaag tatttactga ttatggagat aagctgttag
     1801 cagttgtttt agtgaattta ggattaaatg cagttccaaa aactttaaag gtatcattga
     1861 ctggaggaaa atagatagaa aatggataga tatagtgaga taacaaataa aaatcaaaga
     1921 gaaattgttc tgcttaaagg atttccttgt atatggggaa agtgttcttt ttgtgattat
     1981 atagatgata attctaattt agaagaagag atgaataaat taaatctaaa ggttttaaaa
     2041 aacgtaactg ggaaatatgg agttcttgaa gtcataaatt ctggaagctg ttttgaatta
     2101 cctaaggata ctttagaaaa aataaagtgt attataaaag agaaaaatat aaaaaaatta
     2161 tttttagaaa gtcattggtc ttacaaaaat aggcttaagg aaatgagaga gtattttgaa
     2221 attcctgtgg tatttaaaat tggagttgaa acctttgaca attattttag aaataatatt
     2281 ttaaataaaa atgcaaattt taagactcca caagatgtaa aagaatattt tgattcacct
     2341 tgtattatgg ttggaataaa aggacaaaca aaagatatga tagataaaga tatagagatt
     2401 atcctaaata cttttgaaaa agcgacagtg aatgttttta taaataattc ttctagtata
     2461 aaaagagatg aagagttagt gaaatggttt gttagtaagt ataaattctt ggatgaaaat
     2521 ccaaatatag aagttttata caataataca gactttggtg taggagatta gcattaattt
     2581 agtttaaatt taaaattgaa aagtttctta tgtttttgaa aaagcttcta atagttagaa
     2641 gctttttttg tagagtttta tgttacaata agaaaaaagg aattaaggag caattaaatg
     2701 atgtttgata ggggaaaatg gaataaattg gattataatg aacttttaga atatatgatg
     2761 tctatacaag atataaaata tagagatttt aataaaaagc ttatacctgg aacagaaaat
     2821 ataataggta ttagagttcc taatttaaga aagttatcaa aagaaataag ccaaggaaat
     2881 tggaaggaat ttttagaagt agcagaggat acatactatg aagaagttag gctacaaggt
     2941 atggtaattg gcaatataaa tagtaatttt gaagaaactt tatactatgt aaaaagattt
     3001 atacctaaaa tcgataattg gtcagtttgc gatggatttt gttctgattt aaagtctatg
     3061 aaaaaatata aagaaaatat gtatgatata cttaaaaaat atgtttattc aaagaatcca
     3121 tgggaaatta gatttgcatt agttatgttt ttaatatatt atgttgatga taaacatatt
     3181 aaagaaatat ttgaatactg taataatata caaagtgaag aatattatgt taaaatgggt
     3241 atggcatggt tgctatcaat ttgttttatt aaatgtgaag aggaaacttt tctctatata
     3301 aaaaataata atttagatga tttcacttat aataaaatgc tacaaaaaat aattgaatca
     3361 aatagagtag atttagaaaa gaagaatata ataagaagta tgaaaagaaa gacaagaaga
     3421 tgttgacgga ggaatattaa tgaataacaa tgaagacaga attgacaaat ttttagaact
     3481 tcaaagcaaa ggatttaaat ttgcagagat agctcaaaat ctaaagatta gtcaaagtac
     3541 tttaagaact tttttaaata aaaaaggata taagtcagaa aaaggtaaat acatattaaa
     3601 agacgaatta aattataata aaagtttgga taatagaaac ttggaagaag agaaagctaa
     3661 ggagtatgaa aaaagtaaaa agaatactat aagtaataga aaaaaagtaa gcaccaaaaa
     3721 aaagatttct aatgaaaagg ctttaagtaa aagtgttgaa caaattgagt ttaatgatac
     3781 aggtttaaaa aatgcaaagc caaaacaaaa tgttaagcct aagaaagata gaaaaataaa
     3841 tataactcaa gaagatttag ataagctttg tgaagtatat gattggtatt tagaagtaaa
     3901 agattacaat ataaagccta aaaaagtatc taataaaaaa gatgtacata tagatgatga
     3961 acacatagat gaattaaaaa caacaagaat acgtatagat aaagaaatat ggaatgattt
     4021 tgaaagatta tgtagtaatt cgaattttga aaaacatcaa atacttacac aggcattgaa
     4081 agaatttatg aaaaagtata aaaatctact ataaaatact actatatgag gtgcttaata
     4141 tgaataaaat agtaaaaaaa tcaatttctc taatgattat attaacaata tttattttta
     4201 tgttgacagc atgtgaaaaa gatgaacagc ctgattcagt tgatattcaa acagaagata
     4261 aaaatgagat aaaaatagat gagggaaatg caaaagtatt gaatatagga aaatcagaaa
     4321 ttataaatat agatgaaggt gataaagtag atacatcaac aaaggtagaa aataattcaa
     4381 catttaatat atccaatgtt gaattgatat ataatgaata tgatgagaat aaaaaaatta
     4441 tatcttcaga tagtaaggct ttgttggaca tgactttaat gcctgggaaa gttgcttaca
     4501 tagaatgtgg gcataaaact tttgtaaagg gtgtagaagt ttatgcatat gaatatgagg
     4561 cagagggtaa gattgtgtat gttaatttaa aagaaaatac tattaatata agaaataata
     4621 atattaagct agaaaatagt agtcagtatg aggtgctatc tacatcagaa cttaaaaaag
     4681 taaatgaatc aaatgaggga attacgtatc aagtaaaagt aaaaaattca tcatcaaaag
     4741 atttaggaaa tattattctt aagacagcag aagttaatga aaatggagaa tatttaacag
     4801 taaatcgtgt gccatcatat aagattttaa agccttcaga agagacagat atagatatat
     4861 tgtgttcaac aaaggcaaaa agtgttgaaa tagtgggata tacgtatgat gatataaaag
     4921 aaaaagccaa tgtggatatt gacttaaaat ctcataaagt taagatagac aaataaaggt
     4981 aggggatact tatgaaggtt ttaatagttg aagataataa gatactttta gaaagtgtag
     5041 tagaagaact tagcaagcat tttgaaactg aaaagtgtga agatggagaa gaagctttat
     5101 acttagtaaa tcaaaatata tatgatttag tcattttaga cttaatgctt cctaatataa
     5161 atggatttga tatcctaaag aaaatgagag ttaataatat agatacacct gtacttatat
     5221 tgacagcaaa agaaacatta gatgacaaag tagaagcatt tactatagga gctaatgatt
     5281 atttgacaaa acctttttat atggaagagt tagtagctag ggtatacgct atattaagga
     5341 ctaatggaaa aataaaagaa agaaacggat tagaatttaa atctttatat ttagatacac
     5401 tagaaaaaag agtgtatatt gaaaaagaag aaataaaact tcaaaacaaa cagtttaatt
     5461 tgcttgagta ttttgtatta aataaaggtt caattttatt aaaagagcag atttatgata
     5521 gaatatgggg aattgattca gatgcgacta ttgaaatagt ggaagtatat gtaagtaatc
     5581 taagaaaaaa actaagtaaa tatggatatg ataaatatat taaaactaaa cgtaaggtag
     5641 gatatatatt cgatgataaa taagaatgta tttaccagta ctaaaaatca tttaataaaa
     5701 atgtatatta ttgtagtagg tagtttttta ataatctttt ctatatttat atactcatat
     5761 tttaggggac ttacatatag tggaattgat agtgagataa atgatgaact tgaatatata
     5821 gtctctcaat ttaaaagaac ttcatttttg aacccaataa gactgaaaga tcctaaagat
     5881 atggtgtatg tatatgaaga tggaaggata agttactata ctcaaaatga atattttgat
     5941 gagttacttc cagatagaag actagataaa aaaaattcgt tttttaaata cacagaaaat
     6001 ggatatactt ttagagaatt aaatgtagat gtaggtagat accaaataca gataattaga
     6061 aatatagatt ctgagatgaa ttctttaaga cagcttacat ctgttcttat aattggaata
     6121 ttaatttctg taataattac ttactttgtg gctgtatatc tcactagaaa agctttaatt
     6181 ccaatagaaa cagcatggaa aaatcaagct aagtttatac aagatgcttc tcatgaatta
     6241 agaacaccaa taacaatagt ttcatcaaaa ttagaaagta tgttaaaatc cccagaaagt
     6301 acagtaaatg atgaagttga aacaatagct actgcaatga aggaaactag aagacttaaa
     6361 aaaatgataa ctgatttatt gtctcttact aaagaggact ctattgttaa agttaattta
     6421 gaagaaatag atttagaaaa gttattggaa gaaatatcag aagactatat tgacattgct
     6481 gagtttcagg aaaaaagatt cgtgtttaat tctaaactga agaataaagt tataataaca
     6541 gataaaaata aattaagaca acttatatta attttcattg ataatgcctt taagtataca
     6601 aaattgggtg atgagatagc attagaattg aaagaagata tagaggatga ggtaacttta
     6661 cttataagtg atactggtat aggcataaaa aaagaagaga tacctcttat atttgataga
     6721 ttttttagaa gtgaaaatgt aagaaataaa gatttagaag gttctggaat aggactttca
     6781 atagctcgca tgattagttt aaacttatct attgatataa atgtaacttc tgatgtagat
     6841 attggaacta catttgaatt gtcaatacca aaaaagttaa aataataaaa atataaaaaa
     6901 ttgtttttag aaatgaagaa tgggtatact aattagtata aaatataaaa agaccttata
     6961 aaagtaaatt tatatttata aatttcatag tataattata tagggataat aaagtttaat
     7021 aggaggattt aaaaatggca aaagttataa atacatctga atttagaagt agtgttgaag
     7081 gtagtaaggg agtagttgta gttgatttct ttgcaacttg gtgtggacct tgtaacatgt
     7141 tgggacctgt atttgcagaa ttaggtgaag agatgaagga taaagctaga tttgtaaaag
     7201 ttgatataga tgagagctta gaaatagctc aacaattcaa tgtatctaca gtaccaacta
     7261 tgattatatt taaagatgga aaaccagtag aaactttaat tggattcatg ccaaagaaca
     7321 aaattgaaat gcaagtaaaa tcatatttat aaaaaatggg tgagtattat gagatatgat
     7381 atagctataa tcggcagtgg tccagctgga ttgtctgctg ctataaatgc aaaaataaga
     7441 aataaaacaa ttatcatgtt tggtaatgat aatttaagta ataagcttgt taaagctcct
     7501 tcaatagata attatttagg tttttatgat ataagtggtg atgaattaaa agacaagttt
     7561 aaatcacata tagatagtat ggatatttca attgaaaata aaagaataaa taatatttat
     7621 gctatgggag aatactttac aataatgtct ggaaatgata tgtatgaagc tactactgta
     7681 atccttgcaa ctggggtaga gtatactaga ccaatcaagg gtgaagaaga atttctagga
     7741 agaggtgtgg gctactgtgc tacatgtgat gcaccacttt atagaaataa aaaggtagca
     7801 gttataggat ataatgaaga atctaaagaa gaagcaaatt ttttaagtga actcacttca
     7861 aagacttatt ttatacctat gtacaaaaaa gataatctta tgagaagttc agataaccta
     7921 gatgattcta ttgaagttat acatgacaga ccagtacaga tagatggaga taaacttgta
     7981 aacaaagtat cttttaaaga aaaccacatt gaagttgatg gagtattcgt tataaaagat
     8041 agtacagctc caagtgcatt ggttcctgga atagagattg atggcattca tattaaagtt
     8101 gacaataata tgaagactag tatagatgga tgctttgctg ctggagattg tgttggaaaa
     8161 ccctattctt atataaaagc agctggtcaa gggcaaatag cagctttaaa tgctgtttac
     8221 tatttagata aattaaagag ggcttaatat ttatattgat taacttttta aagatattta
     8281 taaaaaactg tcttgaaata attttattat ttcaagacag ttttttattt ttaagttatt
     8341 aataaaagta ttttaaaatc tatatgtaat tacttttatt aataaaactc aaagttatat
     8401 tagtaaaaat tggaattatt tataatatac tccaaaattt aaaaataatg gttgaaaaat
     8461 ctaattaaga gtactataat ttaattaagt aattacttaa tcaaaacgag gtacttatgg
     8521 aaaacttggt taaaatattt aaagcattga gtgatgaaac tagacttaac atacttatat
     8581 tagtatccaa gagaaatata tgtcaaaagg ggatttctaa atatctaggg atttcagatt
     8641 ctgctgtttc acaacatata aaaatattaa aagatgttgg gataataaca ggatataaag
     8701 aaggatacta tgttttatat catattaata aagaatcatt taatatatgt gtagatttta
     8761 taaattctat gttaagtaat tctagtgaaa gctttataga tgtatttgat gtaaatacaa
     8821 tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa atgttgtaag agaagggaga
     8881 aataataatg aaagtatgta taccagtaga ggagaataaa ggattggata gtaaaccata
     8941 tggacatttt ggttctgctc caatttttgt tgtgtgtgat ttagaaagtg gagaagttaa
     9001 atctttagat aatggagatt tagatcatga acatggaaaa tgtcaaccat taaaagcatt
     9061 gtcagggaca gctgtagatg cagtagttgt tggaggaata ggacaaggag ctataattaa
     9121 attaaatgga atgggaataa aagtttacag agcagaaggt gatactataa gtgctaattt
     9181 agacttactt aaaaatggaa aattagtaga atttccatca gaccatactt gctcacatga
     9241 cggatgtgga catcattaaa aaaaacagct gatgaaatat atacttcatc agctattttt
     9301 tcttttaata aatacgattt tattctttta aaaccaaaat ttagattttt atgttttttg
     9361 tggtatcctt aataatattt caagtttaca agtttactac ttttagattt tttttgtatg
     9421 tttttctata tagtcaatat catttaatat gtcattgagt tctttattaa gtgtttttaa
     9481 atcatcacca tctacattgt agtttaaaaa tagttcctct acattatcaa tgatagaatt
     9541 gacttttcta gtaacttcaa caaatgtctt aaagttacta ctatttttag catttagaaa
     9601 tttttttaaa tcctttgata taaatgatac tatttcatat tcatcattaa atagacctct
     9661 accaatagga tatggttcaa ttctctgtaa aaaatattgg tctttagcat taatattata
     9721 agtataagat atttcccaac cgtctcctaa atcaaacttg ataaaatata atgtactact
     9781 tacttttgat attcttgtgg caccaagctg tcttaaaaga acttcaatcg aatcaccttg
     9841 aacagtatta atattcatat atacacatcc cctttagtat tattatacta tattttttaa
     9901 aattttataa ttaaataaac agtagatata ataatagata taatcataag tggaaaacct
     9961 attttgaaat actccttaaa agagatagga tgaccgtgtt tattacctat acctgctagt
    10021 acaacattag cagatgctcc tattagtgtt ccatttcctc caagacaagc tccaagagat
    10081 gttgcccacc atagtggcat tacatcaata ccttcagctt gcatagtcaa aataagagga
    10141 attagtgttg caacaaatgg aatattatct aagaaagaag agactatagc tgacaaccat
    10201 aatatcaaca acattgtaaa tacaagatgt ccttctgtaa gtccaatcaa tgcttgagcc
    10261 aatttgttta ttatcccaac ttctactaat ccaccaacta ctacaaataa tcccataaag
    10321 aataaaattg tagaccattc aatacctgcc ataatttcat ctacatcttg ttttcctatt
    10381 aaaagcatta tagatgctcc tgtcagtgct acaacagaag aatctatgtg gattgtactg
    10441 tgaaacataa aaccaaaaag tatgaatgct attaatataa ggctttttat aagtagaggt
    10501 ttatctttaa cagatttttt ttcatctagt tttaatattg catttttagc tctttcattc
    10561 acaacaagtt cttttccata aataaatcta aagcatatta tagttatagc aagtattacc
    10621 ataacagcag gacctaaatt tattacaaaa tccataaaac taagattggc agcactacca
    10681 atcataatat ttggagggtc tcctataaga gtagctgttc ctcctatatt tgaagctaaa
    10741 atctgtgtta ttaaaaatgg aactggatta agtcctaata tttgtgtaat aactatagtc
    10801 attggtccaa taagtaagac ggttgtaaca ttatctaata cagctgataa aatagctgtt
    10861 ataatagcaa aacaaatcat tatcttccat gggtcacctt tggctttctt tgcagtccag
    10921 atagctatat attcaaataa ccccgagttt ttgacaacag ctacaaatag catcatacca
    10981 actaatacac caatagtatt gaaatctata tggcttaatc cttcatcaag tgtaagtata
    11041 ttgaatatta tcattaatag tgcaccacca acagctgcta cggatctgtt taccttttct
    11101 gtaataatag ctgccataac tattaaaaat atacatatag ctattatttg ttgaaattcc
    11161 ataatataag tacctccatt tgtattattt tgtgcctaat gctagtacta tatatagtat
    11221 tactttttta ttgttttttc aatggatgta tgaaagtata ttatttaaca aagtttttgg
    11281 aaaattgaca gaatttaaag aaaatttttg ttatttaaat aaattttaaa attattccat
    11341 tgataat
//
LOCUS       DAMJYC010000117        11347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJYC010000117 DAMJYC010000000
VERSION     DAMJYC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.121"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            110..718
                     /locus_tag="PC472_003401"
     CDS             110..718
                     /locus_tag="PC472_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2728703.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            1045..3942
                     /locus_tag="PC472_003402"
     CDS             1045..3942
                     /locus_tag="PC472_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728704.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            4263..4475
                     /locus_tag="PC472_003403"
     CDS             4263..4475
                     /locus_tag="PC472_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2728705.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            4488..4709
                     /locus_tag="PC472_003404"
     CDS             4488..4709
                     /locus_tag="PC472_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2728706.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            4809..6965
                     /gene="feoB"
                     /locus_tag="PC472_003405"
     CDS             4809..6965
                     /gene="feoB"
                     /locus_tag="PC472_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2728707.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6991..7143
                     /locus_tag="PC472_003406"
     CDS             6991..7143
                     /locus_tag="PC472_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2728708.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSECSSSKS
                     CHSAKR"
     gene            7323..8033
                     /locus_tag="PC472_003407"
     CDS             7323..8033
                     /locus_tag="PC472_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2728709.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            8118..8888
                     /locus_tag="PC472_003408"
     CDS             8118..8888
                     /locus_tag="PC472_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728710.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8881..9612
                     /locus_tag="PC472_003409"
     CDS             8881..9612
                     /locus_tag="PC472_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728711.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            9646..10605
                     /locus_tag="PC472_003410"
     CDS             9646..10605
                     /locus_tag="PC472_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2728712.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10703..11299)
                     /locus_tag="PC472_003411"
     CDS             complement(10703..11299)
                     /locus_tag="PC472_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2728713.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4362 a         1165 c         2031 g         3789 t
ORIGIN      
        1 tttaaaaata aatgattaat ccaaaaataa atttgatttt agaaaagtaa attgacatgt
       61 aaagaagaat atattaaact aagtataaaa aatttgctgt tttgtaaagg tggtggataa
      121 tctgataaag tatatacata aaagtgatgt agctattgtt gttttgcatg aaatctatgg
      181 gattaatagc tttatagaag atgtttgtca aaagtattat aaatatggat atgatgtatt
      241 ttgtcctgaa cttctgggag aaaaaagagt ttttccatat tcaaaagtcc aagaggcata
      301 tgattttttt ataaataatg ttggttttga tatttataaa aaagttgaaa agcttataaa
      361 tgatttaaaa tcagagtata aatatgtatt tgttgctggg tatagcgtgg gagcgactat
      421 tgcatggaga tgcagtgaag attctttttg tgatggcata atttgttgtt atggctctcg
      481 gattcgagat tacatggatg taattccaaa atgtcctacc ttattagtat ttgcaaagca
      541 cgattcattt gatgttgatt ctatttcttg ccaactttct aggaaacaaa atatccaaat
      601 tgagatttta gatgcaaatc atggttttat agatagatat tcaaataatt attctgatat
      661 tcaaacaaaa gtatttacta ttaaagaaaa aagttttctg tctaaatgtt tagcatgatt
      721 tagattatta taaaatatat agaaagattt aattaatctg aaaagttttt atgacactat
      781 tgatatttaa aatattagta gtgttattat attttttgaa ataaaattaa tcactagaat
      841 tatagtgtta gttgtgcttt tatgacttat aagaaactaa tattagatag tgtatttaaa
      901 tagataataa taaatttatt atagttgaat aagaatatta tatgagaata ttgcaatttg
      961 atatatttac cattacaata aaagtagtta caactagaaa accaaataag ttaaagtaag
     1021 ttggcaaatt ggaggttttt aaatatgaaa gcatttaaaa agcacttctt gatactttct
     1081 ttgatatttt tacttattat atcatgtttt aacaatatac tatgctttgc tgatgagtct
     1141 gaaaattcaa aaaaaataag agttggatat tgtgatgact atggaataat atcctcatca
     1201 aaagagcatt cttatacagg atatggttat gattatctaa gagaaatctc aaaatataca
     1261 agatgggaat atgaatttgt aaaagggtct tgggaagaat gtttagacag actagaaaag
     1321 ggtgaaatag acttactcgg acctttacag aagaatgatg aaagaaataa actatttaat
     1381 tttcctaaac taagttcagg atatgaatat agtgcactat atacaaagga tagtaacaat
     1441 aatatgtttt atgaagatat aagttccttt aagggaatga gagtagctgt acttagagga
     1501 aactttcata atactgcatt tgagaagtat agagaagaaa ataatttttc agttgaatat
     1561 atatactgta attctattga tgaattaatt gagtccgtca atgaaaaaaa ggcagatgca
     1621 tttgtttgtg gaagtattat aaatgctaaa ggaatgaaaa tagttagcaa attttcagta
     1681 gaaccttttt attttgctac tgcaaaagat aaacctaatt tggtaaaaga attagattat
     1741 gctttaaaag aattaaaaat aaatgatatg tattatgagt tggaattata taaaaaatat
     1801 ttcaaaagag aggtcaacaa ttccatagca tttactcggc aagaaatgaa ttttattaaa
     1861 gcaaacccaa aacttactat ggtatatgat tcagagtggt ctcctgtaga gtattatgat
     1921 aaagaaagca attcatttaa aggtataagt tccgatttaa tgtcaattat atcaaaaaaa
     1981 tgtggtataa aatttgaata tattaaaaca aaaaattata atgagagtct tgattacata
     2041 aaaagtggta aggcaactat gctatgtggc tcaatcaatg aaaatgataa ggcaaaacga
     2101 tttaatatga aattgacaaa tccatacata aatattccaa tgattatggt aggaaaattg
     2161 gataccaatc tcaataatga tttaaatatt gctttaacta gtagttataa gtctattgat
     2221 tcttatatag aaaagagttt tgaaaatgct aagacaacat cttataagag tgtagaaagt
     2281 tgtctaaatg ctataaatga aggtaaagca aacttaatga tacttagttc ttatcagttt
     2341 gatgaacttt taagagaagg aaagagtgag aatttatctg ttatatctgt tttagataca
     2401 agctatggga tgcgtatagg ggtatcaaat aaaactgacc ctatattagt gtctatttta
     2461 aataaatcaa tagacaaaat tactgaagaa gaattaagtg attgtatata ttctaatact
     2521 attgacaaac cttataaagt accatttggt gttattttta aagaatattc aatacaaatt
     2581 atttcttttg tatgtatttt attcttaatt gctattaaat atataattta taataaaaag
     2641 aagaaagagg attatctaag gaagatagca tatacagacc cattgacagg agcagatagt
     2701 atagataaat ttaaaataaa ttctaataaa ctgtttgcta aaaataatcc agaagaatat
     2761 gcgttgtttt atatagatgt tgacaaattt aaatatataa atgatatgtt tggatatgat
     2821 atgggaaatg atactttgat acatatatca aatactattg caagtgaatt aaaggaagat
     2881 gaaatttttg caagagtttc tgctgaccat tttgttcttc ttattaagta taagacagat
     2941 gatgatatta aaacaaggtt aaacaatatt tacaataagg ttcaaatact tagtaatcct
     3001 aaaataaact attataaatt gattttagat tgtggtatat ataaaataag taaaagtgat
     3061 aatgacataa atactattat ggatagagct aatactgcaa gaaaaactat aaaaggtggt
     3121 cataaaaatt catttgcttt ttacgataag gaaatgcaca aaaaaatatt aaaagagaaa
     3181 gaaattgaaa attcaatggt agatgctctt aataatggag aatttattgt ttattttcaa
     3241 cctaaatata gcctttcaga ttatcaaatt ataggggcag aggctcttgt tcgttgggat
     3301 aatccacaaa aaggtctaat accaccaata gaatttattc ctgtatttga gaggaatggt
     3361 tttattgtaa atattgattt ctatgtattt gaggaagtgt gtaaaaagat tagagaatgg
     3421 atggatgaag gacaaaaagt agtacctata tctgtaaact tgtctcgaat gcattttgta
     3481 aatagtaatt ttatagagga gtttaaatta attgtagata aatacaaaat tccgacaaga
     3541 cttatagaat tagagctaac agaaacagca gtattagata atatagaagg attacttgat
     3601 acaatgaaca atttgaaaga aaagggattt gtaatctcta tggacgattt tggtacagga
     3661 tattcctctc ttaatctttt aaaagaattg cctgttgaca tattaaaact agatagagca
     3721 ttttttacag aaaaagatga gagtaataat gaaaaaattg ttatatctaa tgtcattaaa
     3781 atggcaaagg aactgaaaat gaaagtaata tcagaaggtg tagagactat ttcgcaagta
     3841 gaatttctaa agcaaattgg gtgtgatatg gtacaaggat atttgttttc taagccaatg
     3901 cctgtaaaag agtttgaaaa aatcgctttt aaaaaagaat aaagcaatgt gtaaataatg
     3961 acatatataa taaaggtgct ttaatattag ttgagtatac tattttagag catctttatt
     4021 tttgtattaa tttttacgat tttattaata aaataaatca aatgagaaaa aaaatcaaaa
     4081 gaatacaata atcacttgca aaaaatatac tattgtgata gacttaaatc aaaattagga
     4141 attgtaaaaa gaaggaaaaa ataggatatt attttaatag attaagattt aaggtattgt
     4201 aatttatata taagataacg attgcttgta gatttaaatt actttaagag gagggatatt
     4261 atatgatgcc attgatatta tcaaatacag gtgaagaagt agttataaag aaaatttcgg
     4321 gaagtgataa gacgaggact tttttaaata gtataggttt tgtagtgggt acaaatgtta
     4381 aaatagtttc taagatagat ggtaacttaa taatagacgt aaaagatact agagttgcat
     4441 tagataaaaa aatggcaagt agaataataa tatagggagg taatgacatg aacagactga
     4501 aggatattaa gtgtggagag actgtaaaag tcaaaaagtt agaaggagaa ggagctacac
     4561 gaagacgtat aatggatatg gggattacaa gaggtgttaa tatatttata agaaaagtag
     4621 ctcctcttgg tgacccaatt gaggtaacag tcagagatta tgaactatca attcgtaagt
     4681 cagatgcaga aaaaattatt gtggaataat aaagattaat tagagtaggg ttaatgtaat
     4741 tatataatga gaatgatttt ctatatcaca aacagtgatg tatttaaata ataatggagg
     4801 gaatggaaat gtcaataaaa ataggtctca taggaaatcc taactgtggt aagacaacaa
     4861 tgttcaatgg gcttacaggc tcatcacaat atgttggtaa ctggccagga gtaacagttg
     4921 aaaaaaaagg tggaaaatta aaaggaaata aagatgtgga aatagtagat ttaccaggta
     4981 tttattcttt atctccatat acattagaag aagtagttac acgtaatttt atgctagatg
     5041 ataagccgga tgcagtaata aatattgtag atgcatcaaa tatagagaga aatttatatc
     5101 ttacaactca agttttagag ctaggtatac cgaccgttat agctcttaat atgatggata
     5161 ttgtaaataa gaatggtgat aagataaata ttaaagaact atctgaagtt ataggttgcc
     5221 cagtggtaga agtaacagca gttaaaggtc aaggaattat ggaggctgct gaaaaggcag
     5281 ttgaacttgc aagtagtaac aataaattaa atttcaaatt accctttgtt gatgaaagta
     5341 aggatgcaat agaaaaaatt gaaaaaataa tagaagaaaa gactccatat atagatgtag
     5401 aaacacgctg gttagctata aagctatttg agagagatga aaatgttata caaaaacttg
     5461 atatttctaa aacaatattg aatagtattg aagaaataac tagaaattgt gaagatgaat
     5521 tggatgatga tagtgaaagt ataataacag ccaatcgtta tgaatttatt agttcaataa
     5581 tttcaagtat aataaagaag aatcgaaaag gtaaggaaac tgtatcagat aagatagata
     5641 agattgtaac aaatcgtata ctagcattac ctatttttgc acttataatg tggggagttt
     5701 actatatagc agtaagttca ttgggtacta tagcaactga ttggacaaat gatgtactgt
     5761 ttggagaaat tattcaaggc aatgtaagta attttctagc ttctttaaat gtagcagagt
     5821 ggttacaagg acttgtagtt gatggtctaa ttggtggagt tggagcagta ctaggatttg
     5881 taccacaaat catgctttta ttcttattat tatcaatatt agaagattgt gggtacatgt
     5941 cacgtgtagc ttttataatg gatagaattt tccgtaagtt tggtctttca ggaaaatcat
     6001 ttataccaat gcttataagt tcaggttgtg gagttccagg agttatgtca actcgtacta
     6061 tagagaatga tagagataga aaaatgacta ttatgttaac tacattcatt ccatgtggag
     6121 ctaagattcc aataatagca ttatttgcag gggcattatt tggaggtgct tcatgggtag
     6181 ctccatcaat gtatttctta ggtattgcaa tgataatcat ttgtggaatt atattaaaga
     6241 aaactagttt atttgcagga gaaccatctc catttgttat ggaattacct caatatcata
     6301 ttccaagtgc aaaaggtgtt ttaatacata tgtgggatag aggaaaagca tttataatca
     6361 aagcaggaac aattatattt gttgcttgtg gagtaatctg gttcttacaa tcatttaatt
     6421 ggtcattaca aatggtagat gctggagata gtatattagc tagcttagga aatatagttg
     6481 caccaatatt tgctcctctt ggatttggaa attggcaatc atcagtagca acagttacag
     6541 gtctagtagc taaggaaaat gtagttggaa catttggagt tttatttggt atatcagatg
     6601 caacagagca agacccaaca cttcttgctt cagttgcaag catgtttaca gtagctagtg
     6661 cgtttgcatt tatggcattt aatatgcttt gtgcaccttg ttttgcagct attggagcca
     6721 taaaaagaga aatgggttca tggaaatgga catggattac attaggattc caaactttaa
     6781 cagcgtatat aattgctctt cttataaatc aagttggaag tttagtttta ggtactggag
     6841 gaagtattgc tggggcaata atatctatat ttatagcagt agcagtagtc tttgtagttt
     6901 taacttattc aaataaaaat atgaaaaaag aaaaaatggg taaattaagt tatatgaaaa
     6961 attaattcat ataaataagg agtgatgaat atggctacat ttattatagc agctattgta
     7021 gtagtattaa tggcactagc tgtaatgtat atggttaaaa attcaaaaaa gaatggtagc
     7081 agttgtggat gtggttgttc tgaatgctca agtagtaaga gttgtcatag cgctaagaga
     7141 taaataggat aagagcctga taaaaagtta aattgcttta ttatcaggtt cttatttttt
     7201 gtataaataa caaatttcat atttgaagta ggttagacta tgctgtaatt ttactgtata
     7261 attatatata aatatatgat taggagggat tattgctaaa ttaagcaata taagtgaata
     7321 ttatgtttaa atttgatgtt acaaacgagc ttatgtgtat agcaagggga agtggtaaat
     7381 tttttgcaaa aaaaggtgct atggtagcct ttaaaggaaa ttttaatttt gaaaaattgc
     7441 tgttaggtcc aagtaatggc ggtggattgg gaagagcatt attaggtcat gttcagaggt
     7501 cattgacagg agaacaaatg ccaataatgg tggttgaagg tgaaggtgaa atttacctag
     7561 ctcaaaatgc ttaccatgta gatgtaatat caattgaccc aggtgattcc atttatgttg
     7621 agagtgaaaa cttattggca ttttctgaac aattaaatta cagtgtaaaa ttgattggtt
     7681 caggggtaat atctcaaaaa ggtcttttta ctacacattt agtaaataaa acaggtcaaa
     7741 atcaagatgt tgctataata acagatggta atcctctaat tcttgaagga ccttgttgtg
     7801 ttgacccaga tgcattagta gcttggactg gaagagaacc atatcctaaa gtatcaaaac
     7861 tatcatggaa gacttttatt ggtcagactt caggagaatc ttatcatatg gaatttgttg
     7921 aacctggaca aatagtcatt atacaaccat ctgaaagatt gtctggctta aaaacagatt
     7981 ctacaccttc tgatagaatg agttcaggtg gattaagact tggcatagat taaatcttat
     8041 gatatagctt aattattatt taagatatta aaaaataaaa agggtagcag ggatagatat
     8101 gttaaaagga gttttatatg tttgagaaaa ttcataaaag gcttaaagga tattatattt
     8161 ttttaataat tttaatttta tggaagatag tatgtgtttt gggtatatgg agtagctata
     8221 ttctgcctcc tcctgaaata gtcttagata cttttataaa aatgatatat gacggttcta
     8281 ttttcttaaa tgtttatgcc agtgtaagaa ggatattaat aggtttttta ataagttcat
     8341 tgatggctat accacttgga gttttatttg gagtaaataa aaaaatgtat gaatatttca
     8401 aaccactatt tgaatttatg cgaagtacac ctccactagc tttggtgcca atgcttattt
     8461 tatggtttgg tataggtgaa gaatctaaaa ttataattat tattttggca tccttttttc
     8521 caatattttt aaatacattg aaaggtataa aaaattgtga caataaatta atagaagtag
     8581 gtaaatcttt taatttatct aaaaagagaa ttttttataa aataataatc cctaattcta
     8641 tgctggatat tgttgttgga cttagtttag ggcttgggta tagttttagg gctataattg
     8701 gagcagagct tattgcagct tcttcaggac taggatattt gatttctgat ggaaaagata
     8761 tgtctagaac tgatgttgta ttggtaggaa tttttgttat tggattttta ggtatacttt
     8821 cagattatct attttcaaag ataataacaa aatttagcaa agggaagcag gttgatttaa
     8881 atggttagaa gtggattttt gctagagaat atatataaga aatatttagt tgataataaa
     8941 gagcatttag ttcttgataa tatatcttta aatatatcta gtgaagagat aactgttata
     9001 ttaggtgaaa gtggatgtgg gaaaacaacg ctacttagaa ttttagcagg gcttgaaaat
     9061 gctactagtg gaaacatata tttttttaat aatgataaaa agtgtactcc aaaagtagga
     9121 atggtatttc aagaaagtag acttatgcca tggctgaatg taagtgaaaa tatactttta
     9181 catactgaaa aagataatag aaataaagtt gatttagaca agtatttaaa aatgatgaag
     9241 cttgaaaagt ttaaaaactc atatccaaat gaattatcag gaggaatggc tcacagagta
     9301 agtatagcaa gagcactttc cttcaatccc gatattttgc tgatggacga accatttgca
     9361 gcactagatt acttcacaag aagaaaaatg caaaaagaag ttgtaaatat ccataaaaat
     9421 actaaaaaag gtgtagtatt tgtaactcac aatattgaag aagctatgga aatagcaaag
     9481 aaaataattg tttttagtaa aaataaaagg attaaacaat ttagtgtcga agatgaatat
     9541 aatagagatt taactaaaaa ctattatata aacttgaaaa aagaaatatt aagggaattg
     9601 ggggagtttt aattgattaa aagaaacaaa aagattttag cagtaatgac atctttaata
     9661 atggtgttgg ttgggactgt aggatgttca acaacaaatg ataagaaaga tgaacctgca
     9721 aaaaaagagg ctagtttacc taaggaaata aatttaactt atgtaaaatc accacttaat
     9781 gttcctagta tattagaaaa aaaggatgat ttatttggaa aggaatttaa aaaggacaat
     9841 ataaaagtca atttccatga attgacaaca ggaccagagc aaactcaagc attagcagca
     9901 ggtgagctag actttttaca tgcattagga ggaacgtctg caataatagc tgcatcaaat
     9961 ggtgttggtc ttaaaattac gaatatttat agccgttcac caaaaggata tatgcttata
    10021 actaataatg ataatattaa atctataaaa gacttaaaag gtaaaaaaat tgtaggtcca
    10081 aaagggacta tacttcatca actgttgatt gctgcacttg ctaaagaagg ctatactata
    10141 gatgatgttg aatatgtaaa tatggatatt cctagtgctg cttctgcatt agaaagtaaa
    10201 agtgctgatg tagcaatgct tgcaggtcca gtggcattga aaacgataaa ttcaggtgcc
    10261 aaaatgattg taaatggaga aggattagta agtggaataa tagttactgc tgtaagtgat
    10321 gactttgctg aaaaatatcc tgaattagta aaaagattta tgaaggttca tgaagaaact
    10381 ttaaaatata tgaatgaaaa caaggatgaa gtgatggatg ttgtatctaa agaagttggt
    10441 ttatctttag atgaaacaaa agaaatgtat tcttggtatg actttagttc taaaataacg
    10501 gacaaggata taaaagaatt agaagataca caggaatttt taatgtctaa tggaatgcaa
    10561 caaaagaaaa taaatattaa agatatgttg tacaatcaaa attagaatac tttaaaatat
    10621 taaagtggaa aatacaccct tatttattat ggaaactata tgataaaaga gggtgtattt
    10681 ttattattat acaaaaatac aattaaaaac agcttactat tttatctaca cttatgtagt
    10741 ctagtaattt tctcattttc ttacggtgaa gtaaaaactt cgaatatgtg tcctcggatt
    10801 gttttgtgtc accataaact ttccaatatt taatcataga aacttcataa ttactatatc
    10861 ccataaatcc aatcacatct ttaagaggat atcctaaaaa aatacctatt tcatcaggaa
    10921 aaacatcact cttaagttta tctactaaat gctctaaata agcattaaca ttcgtatttt
    10981 gtttgtatcc taaaaacttt aaaaagttta ctgttttttt acattttaat ttttctgata
    11041 atgctttttt atttacaaat aagattttta atcccttttt ttctttatca ataactataa
    11101 aatctatttt cttacattta tatagatatt tataaatatc attaattttt tcctctctat
    11161 ttacatcagt aaacgaaata tttattattt cagcaggttt agagcctaaa aagactggac
    11221 ctaaaacttc taaaagccat tttatatatg atgattcaat tttattttta caacaaaatt
    11281 gtgaattaca acaagacata aaagataacc tccttcaaat gaaaattaat atcaagtaat
    11341 tagatta
//
LOCUS       DAMJYC010000118        11068 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJYC010000118 DAMJYC010000000
VERSION     DAMJYC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.123"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(48..680)
                     /locus_tag="PC472_003412"
     CDS             complement(48..680)
                     /locus_tag="PC472_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2728714.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(752..1105)
                     /locus_tag="PC472_003413"
     CDS             complement(752..1105)
                     /locus_tag="PC472_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2728715.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1183..1386)
                     /locus_tag="PC472_003414"
     CDS             complement(1183..1386)
                     /locus_tag="PC472_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2728716.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            1515..1724
                     /locus_tag="PC472_003415"
     CDS             1515..1724
                     /locus_tag="PC472_003415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728717.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            complement(1776..2006)
                     /locus_tag="PC472_003416"
     CDS             complement(1776..2006)
                     /locus_tag="PC472_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728718.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2008..2415)
                     /locus_tag="PC472_003417"
     CDS             complement(2008..2415)
                     /locus_tag="PC472_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2728719.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2775..3224)
                     /locus_tag="PC472_003418"
                     /pseudo
     CDS             complement(2775..3224)
                     /locus_tag="PC472_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3238..4164)
                     /locus_tag="PC472_003419"
     CDS             complement(3238..4164)
                     /locus_tag="PC472_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728720.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4289..5071)
                     /locus_tag="PC472_003420"
     CDS             complement(4289..5071)
                     /locus_tag="PC472_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2728721.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5227..5847)
                     /locus_tag="PC472_003421"
     CDS             complement(5227..5847)
                     /locus_tag="PC472_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2728722.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5935..6633)
                     /locus_tag="PC472_003422"
     CDS             complement(5935..6633)
                     /locus_tag="PC472_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2728723.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6734..7549
                     /locus_tag="PC472_003423"
                     /pseudo
     CDS             6734..7549
                     /locus_tag="PC472_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8221..8988
                     /locus_tag="PC472_003424"
     CDS             8221..8988
                     /locus_tag="PC472_003424"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2728724.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9316..9993)
                     /locus_tag="PC472_003425"
     CDS             complement(9316..9993)
                     /locus_tag="PC472_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728725.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10143..10460)
                     /locus_tag="PC472_003426"
     CDS             complement(10143..10460)
                     /locus_tag="PC472_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728726.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10482..10820)
                     /locus_tag="PC472_003427"
     CDS             complement(10482..10820)
                     /locus_tag="PC472_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728727.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3681 a         1743 c         1570 g         4074 t
ORIGIN      
        1 taaaatgatt attaaagtaa agtttaatat aaataaatta taaaatatta aactaattta
       61 tcaatatttc ctgattgaat atgcttaatg tttgattgaa tttttttata tggccaatcc
      121 caccatttaa tttctaataa ttttgaaatt gtatcattat taaatctctt ttttataatt
      181 tttgcaggta ctcctccaac aatagaatac ggtggaacgt cattagtaac tactgctctt
      241 gttccaataa ttgcaccatc accaatattt acacctgaca taattataga atcataacct
      301 atccacacat catttccaat tataatgtta cccttattgt cccaagcgtc cgttatatgg
      361 ttgatatcga gtttccattc ttcataaaag atgggaaaag tatatgtgga ttttgatttc
      421 attgagtgat ttccactagt cattagaaat tttgcattac atgctattga gcaaaactta
      481 ccaattatta atttatcatt gtttataggg taatgataca atacattgtt tttctcaaaa
      541 tctcttgggt cattataaaa gtcattatat attgtgtaat ctccaacttc tatattttcc
      601 cttgttatta catttttaag atatacagtt tgataatcat tacttcttgg atatacctta
      661 tttgaacttg gaatagtcat atttaaatct ctcctttaat atatattatg tttttgacta
      721 ttccactact acaatacatc tcttcacaga atcactcctt gttaagaatc ttcataagat
      781 aatggcgtcc ttttttagtg ttaagtcgga tattctttct tagtccattc tttttagcgt
      841 ataactgaca aactgtcatt ttcttaccaa tagtgtcaat gctatcatca aggtcatttt
      901 gtattgcttt ttccttatcc cttaatgata tatcatcacg ttttcccttt gggggtttat
      961 cagtaggtac aagtttccaa gagtaaacaa attgtggttt accaaatgaa tatacatact
     1021 tatatgcgta tcttccatct tttctctggc tctctccagt tcttaaaatt ctatctttat
     1081 tatctcgtct tttttcagtt gacatgataa gaagctcctc tcaataaccg aattgagctt
     1141 gatacgtcat atgaatatta taacatataa ggctcaattt cattagataa cgtcaagtgt
     1201 atcaacgatt ttctcaaatt gttttctctt tatttgaatg cgattgctat tcataatgac
     1261 ccagccagaa tcaaggtttt catcagcacg tttacgtaat tttttctctc caatacgaaa
     1321 gtattttgac gcttcttcaa tcgtcaaggt atatttttcc caaataggta catcagtatt
     1381 gttcatataa tgtatcactc ctttctatgt tcgtagttca gaagtagata gatggctttg
     1441 ataagctcca ttggtatttc aaccaatccc attcttatgg acagaaccgt gtcatacagg
     1501 tgtatcatta tactgtggta caggttacgg caaaacaacc gttccaaagg ttattcacaa
     1561 atcactatat agtagctctc ttgattaagg tcatggcgta tagtatttgt tggctatctg
     1621 tatcacaaac gtatctatct actttattca gttgtcaaag aacagtcggc attgatttgc
     1681 ctataaaaaa tcacaatagc ttgtgggctt ttatagacaa ataattagtg attctccatg
     1741 taccaaatag cttgatacat ggagagaggg cgagattaag acaaattaaa tctcaaaata
     1801 gcagtgatta gttttgttcc tagcattcgc tttgtttctt cttctacatg aatataggga
     1861 tttccgtatt catcaaataa agttttctgt gaaagacgaa tgatatagtg ttcaaaatgt
     1921 ttaagtatta cgtttattgc atttacatcg ccatgtacag caagtaaaat aattgtaaaa
     1981 ggtaatactt ctttagtagg tttcatttca atgttcctgc atgagttctt tcaactcttt
     2041 tagtgcattt gttttgtgat aatgtatggt attttctgct aaatccatca atgttgctat
     2101 gtcagcattt gtcatgttga aaaagaatga aagtagaatt atatcttgtt ttctttttgt
     2161 aagttttccg attgcttgcg ccattaatac atcttcaatt cgtatgttgt agtcgtaaga
     2221 aaccatacag atgtagttag aatcataatc atctaaaata ctcaatttac ttaattctgc
     2281 aagtgacaaa gattcgagag ataccaaata atcatttctg cgtttatttt cgtcataaat
     2341 atctctagca tagttacgca aaactgtttt acagaaactg tcgaattgat gttcaattgt
     2401 cttttcatca accataagat ttcaccctct ttccatcgca aggagttaaa taaccttgct
     2461 tactcctatc acggtgggag ggcgttaaat cttcgataaa tttcaaaaaa aagagaaaaa
     2521 aatacacact ctgcctatag ggcaaaaatg tgtattcttt aaaacgtatt taatttatat
     2581 tggttgtaag ccaccaccat attgatgtat ggaaatatgc attggctagc ttttcataaa
     2641 aatacaaaga tgcataagaa attaatagag gggaaaaact tattctgtta ttaaaaagta
     2701 ggttaattct aataaatgct attgcggtcg aattaaacta tatacatggc aatcaatcat
     2761 gtttctattc tttattatga attgtgacag tattcttcaa tcttaaagcc acatttttcc
     2821 aatactttac ttgactctct attgtaggaa tatacattag catgaatttt gttaaggttg
     2881 tatttttcaa aggcaatttt gcatatttct tttactgcat ttgttgctat ccctttgcct
     2941 caatagttcc tagcaagcca gtaacctaat tcagcatttt tattaataga ggtatcttct
     3001 tcaaatgtta ggctgatact tccaatagcc ctttcttgat aaaaaatagc atagctaagt
     3061 tgtttaacat ctgaagttgc caaacaaaaa ctaacaaaat ctctagcgtt ttgctcccta
     3121 taagggtggg ggaagttgtc acgaagattc tttgcaattt ctttatcatt cgcaaactca
     3181 actaaatcgg taatataagc ataattccac tctataagtt ccatttttct tctcctttta
     3241 tgaaacagct acaagaaatc tttctaagcc aataccactt atcaaacagc gttctctttt
     3301 atttccaagc atttgataag tccagtcaac aaatccaccg tcaactattt caacggtttc
     3361 atttccttgt ttgaccgata atttgaaatt gataccttta taataattat tatcaacatc
     3421 atcattatga attgataact ctatattagg aaaaataagt ttgatagttt caaccattct
     3481 gtcgaaaaat ccgtcattat ctttgtatcc gtttcttttg cgtagaataa tagagaaacg
     3541 aacattttct atttcactta atatatcttt ataatagttt aagtgctttt ctaacaacat
     3601 tttttcacaa gtgtaagacc ctgtatctat accagatgaa accatacaat ataagccaaa
     3661 atgtgaatat aaacctttac ctacaaaatt ttgtgctctt attaccctag cagtagtagc
     3721 catgtggata ggtatggtat tattttcttc ttttcttttt aatttatcag caataattat
     3781 tgccaacata tttgaggggt ctgcaagtgt ttctgtccct cgtagagcac ttactacgtt
     3841 atattgatct acggagccaa aaacgctaca gctacctaat ggggcagagg gagaaagcat
     3901 aatcgtcttg atatccatat tgaatgcttt tctcaataat tttgattcca aagcatgaat
     3961 ttcttctgga tctataccgc tagggatagt aaatctattt agttggtatg actttagaat
     4021 atcagttgca gttaaagtat ttgcttgtct atcaaacact tctaatagaa gtgagttcaa
     4081 atctgatttt gatagagctg ataacttatc aactatattc ttatccccaa ttttatttgt
     4141 aattctactt aaaatatcac tcatagttat cctccatact gttactttca aaataattat
     4201 agaattgtta caacaactaa tctaggtcat ttgaccaaaa atgaaaaaac actgaatatt
     4261 tactattcaa tattttttta ctttgggttt atttgattaa atcttgaaaa tactgtaatg
     4321 caaccctttc agcttcaacc tttcggacaa taggactttg tgataattcc tctcttaact
     4381 caagatacag gttgatagta gcctgcaatg cattaacaca gcttttatat tcatggttag
     4441 caaccgtctt ttctaatgct tcaatatatt ggggtgcgtc caattcaatt tttctaactc
     4501 cacgaggtaa tttacctaag agaagctgta ttaatggggc aagtactgat tgtcttaaaa
     4561 aagataaaaa atcaattgtt tcaaatagtt ctctacgtcc aatcttagtg caagcgtaat
     4621 gaacccaaat ccaaaatcta tcttcaatcc actgtaagtc tggagttggg aagtatgctt
     4681 cttttttaga ataaatttct gttatgcagt tgttttcttc atagagaata gctgaatctt
     4741 caaccctatc tagtaaacca tctaaggttg taaacttaaa atcaacatga agaacagggg
     4801 aatcatagag ggaaattatc aatcgaggtt ctccaacgtg ttcgccagta aaagcagaaa
     4861 ctaatgttcc aaactgttcc acaataagta atctctcttc gataacttct tcataggatt
     4921 tatcatctgc tacaatcaga aaatccaaat cagaatattc atctaacgat tttgtgatat
     4981 aagaactgct aattgcaagt cctattatcc tactatctct ttttgaaaat tctaatacgt
     5041 tttgtatcat tttctgttgt gttttagtca taatataaca cctctcactg tcttttatta
     5101 gctatcaatc caacataatt atgcatagga gtattatata atgaagaaat aaaaaaaaat
     5161 aggtcacttg acccgaaata aaaaagcatt gaataattat tattcaatac ttttttgaaa
     5221 ttatacttat gttgtaggaa gattctttaa tacctctcgc tgattaatta cactaaatct
     5281 tttgttttca taagcaatta gatgttcttg tacacattca tttatagcac gattaatgct
     5341 tctaattgga gcgtttactt cgctagcaag ctgttttttg gtaagcagat ttaattcact
     5401 tctataatag tgtaaggata tacacctcag aagtcgttct ttaacactta aagatgataa
     5461 ttcttcaatt aattcagcat ttacaactaa tttactagat atttctcgaa tcagaaattt
     5521 tgtagcttca aaatctcccc ttaaccagtt taaaaaatca tctctatgaa gtttatatac
     5581 attacaatct gtgaaagcga caatttcaac gggttttttc ccatcattaa attgttccat
     5641 ttctccaaaa aaactaccac ttctataaag atgaatcgtt gagatgtttc ctttcacatt
     5701 taagatgaat gcttctgcaa taccgtcaat cataagaatt acatattcat tttcttgctc
     5761 agcaaaaaga atagtctctt tctttttata cgttttttta acaagcttct ttttaatatc
     5821 ttcattcata aaatcaatta aatgcatatt catccctcct taccaaatct ttgattaaat
     5881 tatatcatct ttcaattagt taggtaagat agtaaaaata tatttacata gaaactattc
     5941 cattattatt ttccagttgt catttttctg caaaaactta tcactgtcac gactggagat
     6001 accagtaagg aatgttagga tatcaatcgc taaactttca gaatcctttt tacggctcaa
     6061 aatgacgtaa gggtcaagca atcctttgtt ttcatcatta ctggttaaat tgacaatgtt
     6121 tatctcatgg gctatttctg gtagcgtttc tttccatttg ccgcgttctg ctttagggtc
     6181 aactatgacc gcctgtccgc cgaatagtac cgcatagtaa acaagcatat tgttactgaa
     6241 taatttacca ccacccaatg aaccgataaa cgcagacgct aacgcattgg taatagaacc
     6301 ctttacccct tgactagcaa gggcaggttt tagatagaca ttctttccag tgtccagatt
     6361 gtagccaaga tagatatcct catgttcgcc aagggtttgt gtcgctccaa aaccaagtcc
     6421 agccaaaaaa tccgaagtga catattgaat ataatcattg atgtaacgtt tgctgacagg
     6481 aataaattcg ccatgcaatc ccaacatatc cccaaaggga cgcacgagct tgattgataa
     6541 gtcgtcgtaa aagtctttga cttcatcaca acgccttttt agctcgtcaa gattctttgt
     6601 agatacacgg acaacatagc taagtttata catagattct ttacagttaa aagtctgttt
     6661 ttaaaaaaat gtataaatta taaatcaaaa tatagatact atcaatgacc cactaggtgc
     6721 aaaaagcatt gatatggtta aattatcaga cgctaaaagc tgggctatcc gaatgagtga
     6781 agatgggtgt gcttataaat ccattgacaa ttataagcgc tcactgaaag cgtcgttttt
     6841 catggcaatt caagacgact gtattagaaa gaatccattt aatttttagt taagtgatgt
     6901 tttggaagat acaacagaac caaaaatcgc tctaacacca gaacaggaaa tgggcttatt
     6961 atcctttatg gggagcgata agacttattg taaatattat gatgatgttg ttatcctctt
     7021 agaaacagga ctacgcattt ctgaattatg cggtttgact ttagaattgg atatgaaaaa
     7081 taaagccatt atggtagacc atcaattgtt gagtgatact aagattggct attatgtaga
     7141 agttccaaag accacacagg gtaagcgaga aattccaatg actgaaagag cttatcaatc
     7201 atttgaacgt atcgtggaac ggcgtaagaa cgctaaagca gaaccaatca ttgttgatgg
     7261 ttaccagaac ttcctatttc ttaaacaaga tggattacca aaacaagcaa ctcattatca
     7321 aagcatgtta agagcgttag tcaagaagta taataagacc catgaggaca agctaccaaa
     7381 gattacgcca cataccttta ggcatacact ctgtacaaat atggctaaca aaggaatgag
     7441 tcctaatata ttgcaatata ttatggggca taaaaacatt actatgacgc ttggctatta
     7501 tgcacatggt tcatttagtt cttcgagagc tgaaatggaa cggttataat cctagtagta
     7561 tgactagagt ttactactta tttactactt ttgatggcat tcttatacgg ttaaatgcca
     7621 acttatgcaa agtatcttcc aaaaagaaaa tgccgatact ccttgtagat acaggatata
     7681 tcgacattta ccaacttatg aaaagataat caaatttaat ataagttttt caaataaaaa
     7741 gataccaaat tctttttcga gaatctatat aacattactt aagcttggtt ggggtataaa
     7801 caattgagta gctacttttg tatggtgttc aactttagtc aaaactctta agtaatgcaa
     7861 atgatttaga ttcattaata ttcaatctct tcataagtag tgaaattcga gttatttctg
     7921 cataattata ctatgattta tagattaatt ctattagcat ccagaataat attgtttata
     7981 tgagtaaaat ttaaagaata ttgtgaaaat tataggatgt taaaataaat ttaggtaaaa
     8041 aagaagtgtt tgtatattat agagtacctt ttctaaaata tgtgggattg ttttaagaat
     8101 tagaataaaa attattttta aattagtatt tttataatgt atagtatttt taatgtataa
     8161 ttaaattgtg cagaaaatag aatttaaagt cctatagaat ttctgattat ggaggagaat
     8221 atgaaaagaa aagttcaagt aaaaaatatt actattggag aaggtagacc taaaatttgt
     8281 gtaccaatta ttgggaaaaa taaaaaagat attataaaag aggctaaaga gttaaaagat
     8341 gcatgtttgg atattataga gtggcgagtt gatttttttg agaatgtaga aaatattaaa
     8401 gaagttaagg aagttctcta tgaattaaga agttatatac atgatatccc tttattattc
     8461 acatttagaa gtgtagtaga agggggagaa aaattaatat caagagatta ttatactact
     8521 ttgaataaag aaatttctaa tacaggttta gtagatttaa tagatgtaga gttatttatg
     8581 ggagatgagg tcatagatga agtagttaat tttgctcata aaaaagaagt gaaagtaatt
     8641 atatctaatc atgattttaa taaaactcca aaaaaagaag agattgtatc tcgtttatgt
     8701 agaatgcagg aattgggcgc agatttaccc aaaatagctg ttatgccgca aaatgaaaaa
     8761 gatgtgttag tgctacttga agctacaaat gaaatgttta aaatttatgc agatagacca
     8821 ataataacaa tgtcaatgtc aggaatgggt gttataagta gattgtgtgg tgaaatattt
     8881 ggttctgcat tgacttttgg agctgcaaaa agtgtttcag caccagggca aatatcattt
     8941 aaagagttaa attcagtact taatctatta cataagagta ttaattaaat taattatata
     9001 gaaatattag acggtatttt tgaagatata aattaaatat aaaaaacttt tcagatagta
     9061 aaatttaaat tgggcaatta tatctgaaaa gcaactattt agtgtgtatt ttgaaagtga
     9121 tatagaaaat cttataaatc aactatggca tatttaaaat atgccattaa aacctactag
     9181 acattagatt atagatggta tactagcatt tctattattt atagatagta agttttcata
     9241 ataactgttt tatctaaagt agaagatata tttgtattat gtatatttgt cattattttt
     9301 ttgtaattat gtatattaat ggttatatat gtaatataat attatcactc tattttttgt
     9361 atattgtaca ttacaacatt tatatctatc atcatcaatt acagtagaat gtatgggata
     9421 cttattttca cttaaccaat atttttgcat attctttgtc caagactttg gaaacttcaa
     9481 tactttcttg tttattataa catcctcatc ttcaaaaata ttaacttctc tttctatttt
     9541 tatttttctg ttgtatatct tattgataga ttctttgtcg agttttatgt ctctatacat
     9601 atcagggcaa tagcaacaaa cataatttct tttttttcga cgtttaaatt tcacaacttt
     9661 ctctatgcta atttctaatt tttttgtacc atcttttata aatttaatat cttctatgtt
     9721 ctcgcttgaa aaaatttctg tcaatcttaa agtatctaca gtttctctaa ttatttgctt
     9781 tttataagaa gagaatccat atccaaaaga aaggtttaat ttattaggga tgtagccatt
     9841 tttatattta aacatcttaa ttttttccaa ttcagcatta tctttaaatt tcaacttttc
     9901 atacctaaaa gttactttgc taaaaagagt atattctaca tcaatatatt cttttgcaat
     9961 cttaaattta tcgctacctt caatcacaat catagcctat ctccaatcgt tttatattaa
    10021 aataaggcca tagaacatgg tctaatagta ctcattagca ctataagaca atgttaacac
    10081 aaaaagacaa aaaaaggtta tttatttcaa ataacctttt tttttatata aaatataaag
    10141 aattatattt tattcttttt tatgatgtct ataatctgtg atatcagatt tagttctctt
    10201 gaatttaagt cttttaactc ttcatttata tcattttcta aatatgtttt tccagtggaa
    10261 gtaatatctt ttataagaac atctgcagaa atatttaata tatttacaaa ttttatgaat
    10321 gttttaagac ttggattctt tttaccactt tcgatttgag ttatatacca aggcgatact
    10381 ccaaccatat ttgcaaattc ttttgaagtt aaatttgctt ttttccgttc ttctttaagt
    10441 cttttaccta tataattcat tattgttact atttccctca cttatatttc tttgtattta
    10501 atgatgcact ttattaactc tagcattgca atttgttctt tttcagttaa tttattaatc
    10561 atttcataac attctaaagg tacagagtta tcaacgcctt ctgttgtaat aatatttgca
    10621 aataacaatt caagtggtat gtctaaagca gtagcaattt tcattataat attgatagat
    10681 gtatttgtct cagctctttc aatctgacca atatatgttg gatgtaaacc tgccttttct
    10741 gctagaaact cttgactata tcccaatttt gttctataga cttttattct atctcctatt
    10801 atttttccta tatttgccaa ttattatccc tccactttag attatattca tagtttaagt
    10861 tgttttatat agactattta tactttttta gtctaaatat agactatata tgtttttata
    10921 ctgaaataag tgctatttat ttttgtatag tctgtatcta caataaacaa atatggataa
    10981 atcatatttt taattagaga aatattttca taatttcata gccatactac aataataaaa
    11041 cgaattaata atatgttaat tatagcat
//
LOCUS       DAMJYC010000119        10982 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJYC010000119 DAMJYC010000000
VERSION     DAMJYC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.124"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(209..1939)
                     /gene="lpdA"
                     /locus_tag="PC472_003428"
     CDS             complement(209..1939)
                     /gene="lpdA"
                     /locus_tag="PC472_003428"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2728728.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2106..3152)
                     /locus_tag="PC472_003429"
     CDS             complement(2106..3152)
                     /locus_tag="PC472_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2728729.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3165..4151)
                     /locus_tag="PC472_003430"
     CDS             complement(3165..4151)
                     /locus_tag="PC472_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2728730.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4176..5138)
                     /locus_tag="PC472_003431"
     CDS             complement(4176..5138)
                     /locus_tag="PC472_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2728731.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5176..6129)
                     /locus_tag="PC472_003432"
     CDS             complement(5176..6129)
                     /locus_tag="PC472_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2728732.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6574..7947)
                     /locus_tag="PC472_003433"
     CDS             complement(6574..7947)
                     /locus_tag="PC472_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2728733.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWAKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGSINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQDYIG
                     VIFSSAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8044..9690)
                     /locus_tag="PC472_003434"
     CDS             complement(8044..9690)
                     /locus_tag="PC472_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2728734.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMRWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9712..10878)
                     /locus_tag="PC472_003435"
     CDS             complement(9712..10878)
                     /locus_tag="PC472_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2728735.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
BASE COUNT         3275 a         2200 c         1172 g         4335 t
ORIGIN      
        1 aaaatgataa tgttatttta tattactatc ttttttgtac aacaaaaact acaaggattc
       61 tatattctat atttttccat aaaaaatacc ccttattatg caaataaatt atttgcataa
      121 taaggggtat ggctaagtaa gtgttttaaa attttatgat ataaattaat attaataata
      181 gacttaaatt aataatttat gtttagattt attttttagg taagtgtaaa cattctccat
      241 ttacatctgc caaagcttcc attaaagctt ctgaagtaca tggatgacca aatatcaact
      301 cacttgcttc atcagtaggt atctctaacg ctttaaatga tgctgcatga tttataagtt
      361 ctgctacacc acatccaaac atatgtatac ctaaaatttc tccatattta gcatctgcta
      421 ctaccttcac atatccttgt tcttgaccag aagctaaagc tcttccattt ccagcaaaat
      481 taaacttacc aacttttaca ttatattttt ttctagcatc ctcttctgta ataccaactg
      541 atgctacttc tggaattgta taaacacaac taggtaatgc acctaagtca acttctttat
      601 tcacacctag agcatttgaa gctgctacct ctcccatttt aaaagcagca tgagctaaca
      661 ttacacctcc agtaacatca cctactgcat atatgcttgg tatacttgtt tccattttac
      721 tatttacaac tattgaacct ttatctattt taatatctaa gtcctctatg ccagaaagat
      781 tagcttctct tcctatagca tatagacata aatctgcttt tattggctct tcaccttcaa
      841 tgcacactaa tatgttattt ccttcttctt taaattcaga aactttcttt ttagttaata
      901 catttatacc ttttttacta agagaatatt ttaaagattc gcttaactct ttatccattc
      961 ttgggattac tctatcttcc atttctacta tagttacttt agaacctctt gaattgaata
     1021 tctctgcaaa ttcacaacca atcactccac caccaattat tactaattct tctggcactg
     1081 tttccaaatc taatgcttct gtactagtta ttattaaatt tgattctatt ccttttatag
     1141 gtaatatcct tactttggaa cctgtagcaa ttattatatt ttctgtatct aaaacttttc
     1201 catctgataa aattacttta tgctcttctt taacactagc tttaagatta aacacatcta
     1261 catctctact ttttaataat ccgccaacac cagctgtaag ttttttaact actctatttt
     1321 tatattttat ggccttctta atgtcctgtt ctttatctac agttactttt acacctctct
     1381 ttgataattg gtcaatttct tctaatattt ctgctgtttt tatataagtc tttgtaggga
     1441 tacaacctct atttaagcaa gtacctccta gtatattttc ttcaactaaa gcaactcttc
     1501 cacctaaaag agctgctttt aatgctgata aatatccccc aggtcctcct cctattacca
     1561 ctacatcata atcatgatta cactcttttt tgtttatttt ttcttcttta aattctatat
     1621 tttgattctc ttcaactata tcttcttttg aaacatttcc agataaatat ttagctttta
     1681 cttcctcttg attttcccct ttttctgcta ttacacctat tactgtaaat actggtaata
     1741 cttcaccttc ctctttatgt atgattacag caagtgtacc ttctccttct gactctatct
     1801 ccatattaac tttatctgtt gttatttcta gtataggttc tccaatcttt acttcttcgc
     1861 cttcttgctt tagccaagat actatagtac cttcttccat ggctactcct gcttttggca
     1921 ttattacttc tactgacata ggctctcctc ctatttattt tcttatattt taattaagca
     1981 aatcatattc acatatagtt actgttaaaa ataattctta atatataaaa gctttaaatt
     2041 tactgaaaca attataataa tataatctat tcaaactatt tatatcagta acaaaatatc
     2101 aaaacctata caagcattga taaaggattt tccaacaatt cttttaaatc ttttaagaat
     2161 tttgcagcta ctgttccatc tattactcta tggtctgatg ttaatgataa gttcattata
     2221 ggttttattt tagcctctcc atttactgga acaaacttat cttgtgtagc acctacccct
     2281 aagattgcac ttgaaggcat atttattata ggtgtaaatg tagttatacc atacattcca
     2341 acattactta ttgtaaatgt attaccttct tggtctgctg gcataagttt ccctgtcttt
     2401 actttttccg caagttcttt gctttctttt gctatttctt ttaaagactt tttgtttgca
     2461 ttttttacta ccggaacata taagccttca tctaaaccaa ctgcaatagc tatatttaca
     2521 tctttatatc taaatatacc ctcatctgtc catgctgaat ttaaggcttg atgatttgga
     2581 agtattttag atactgccat tactattaaa tctgtcatag ttaattttac tccagttgat
     2641 tcttttactg tgtcgattaa ctttgctcta agtaccttca attctgtagc atctacttct
     2701 atattgaatg taaatacagg agcactaaaa taactttctg acattcgttt tgctacagta
     2761 gctctcattg gatttggctt ttcaaatatt ccttctactg tgttaaacat tcttgaattt
     2821 tcattttcaa ttttcagctc ttttttctca ctagatttag atgtattagt tgatacaata
     2881 gtttcttttc cagttaattt ttccacatca cactttctta tctttccatt atatccagta
     2941 cctacaatag tttctaaatc tatatttaaa tcttctgcta ttcttctagc taaaggagta
     3001 gtatttgctt tattatcttt taaataattt aacacatcta ctaaatgaat ccttccattt
     3061 tctccacttc caatcagcct atctaatttt atattgtctt ttcttgcctg acttctagct
     3121 gctggtgtgg cttttatttt atttgccacc atatctatcc ctccttatct aacaattaaa
     3181 gattttattg cttcttctat ttcatcaact cttgggacaa ctgctttttc taactctgga
     3241 ttatatggta ttggcacatc ttttccacaa attcttttta ctggagcatc taaataatca
     3301 aatgattcac tttctgttat cagggctgat atttctccac ctagcccacc agtcttagct
     3361 gcttcatggc aaattaaaac tctcccagtt ttacatacac tctttactat agtctcttta
     3421 tctaatggat ataaagttct taaatctata atttcaacat ttatattttc ttttgataga
     3481 gtctctgctg cttcttcaac actttgtaac attcttccat aagtaattac tgtaacatca
     3541 gtaccctctc ttttaatatc tgcctttcca atttcaatta cataatcatc ttctggcaca
     3601 aaaccttttt ttctgtataa caatttattc tcaacaaata tgactggatt attatctctt
     3661 atagctgctt tcaacaatcc cttagcatct gctggtgtag aaggtgcaac tacttttact
     3721 cctggtacat gacaaaacca agcttctaaa ctttgtgaat gttgctctgc tgaaccagtt
     3781 ccagaacctc ctgggcatct aacaaccatt ggaacttgtg cttttcctcc aaacatatat
     3841 ctcatttttg ccgcttgatt tacaattgca tccatagaaa ttgttacaaa gtccataaac
     3901 attacttcca taataggtct taagccagtt gctgctgcac ctgctgccgc tcctgctata
     3961 gctgcttctg atataggagt atcccttact ctttctggtc caaattcatc tatcatacca
     4021 actgatacac caaatgctcc tccatatatt cctatatctt ctcccataaa tattacattt
     4081 tcatctcttc tcatttcttc tgacattgct tcctttattg cttgtgcata tgttaattcc
     4141 cttgtactca tttttatttt cctcctttac cattcttagt ctgcatatac atcctctaat
     4201 aaagactcta tttttggatt tgggctattt tgagcaaact ctactgcatc ttctattgat
     4261 tgctttgcaa attcttgaat cttatctaat tcatcttcat tagatagatt attttctata
     4321 agataatttt ttaaaaattc aatagggtct tttgctttcc atgattctat ttcttctttt
     4381 gttctatata catttgcatc tgattttgaa tgacctaacc atctataagt tctactttca
     4441 attaatacag gtccttctcc tcttctacac ttctcagcag ctttttgtac tgtttcgtat
     4501 acttctattg ggttatatcc atctattgat attccttcta ttccataaga agctgctcta
     4561 gtggcaatac tttctatatt catatgacgt tttatgcttg ttgacatacc gtataaatta
     4621 ttctcacagt aaaatattat tggcaatttc catattgaag ataaatttat tccttcatga
     4681 aaagtacctt catttgatgc tccatcacca aaactacata aaactatttt gcctgttttt
     4741 ttatactgtt gtgtaagtgc agctcctgga gctattgtaa gacctccacc tacaactcca
     4801 tttgctccta agttacctga ctcaatatct gctatatgca ttgagcctcc ttttccctta
     4861 caaaatccag tttctttacc cataagttca gccatcattt tatttaggtc tattcccatt
     4921 cctataaatt gactatgtcc tctatgagtt aatgatacta aatctccttt ttctaaagcc
     4981 attacagccg ccacacttga cgcttcttga cctacagata agtgagttgt tccatgaacc
     5041 atacctcttg caaaaaacca actaactttc tcttcaaatt ttctagcctg attcattctc
     5101 ttatacattt caagcaatgt ttcttttgat attgacatat acattccctc ctgttaactt
     5161 ttatctatct ttctattagt cacaatcttc taataattta agtgctaaac tgtgattaat
     5221 tgctaaacta tttataaatt tcgcttttaa tgatgccttt attgcctcta ttttttcatc
     5281 tcctgaagcg acacctattg ttctttttat cttttttaaa tcttccagtt tcactcctaa
     5341 tactctttga ttaaattcaa gtatagaagt ttttccttct ttatcaaagg cttgtaaaca
     5401 aattgcacct attgagttgt atttttttaa atcttctata tctgcttcat tcatgtatcc
     5461 agaagccatt attgttgagt ttaaactcat aggatttcct atacccacaa ttgctatagt
     5521 tgttttatct attaaatcta gcacttcttt aattttttcg tctttgcaaa gtttctcttt
     5581 tgtagacaag tcagacataa cagctggagc atgtaataat ttaaaatcac ctccaaaagc
     5641 cctcgctaaa cttacggcta tttgatttgc atgaatatct atttcattgt ctccaatccc
     5701 tcctaataga ggtataaatg taacattttt acagttactt ttttccacat atcttggtat
     5761 ttcttttagt gttgttccta ttgaaacacc tattatatca ttttccttta ctactctagt
     5821 taaatattca gatacagctc ttgctagttc ttgtttttgt gttagtgcat cctgtttatc
     5881 atctactact attacttctt ttaatttata ttttctctcc aaacttctct ctattttttg
     5941 gtaatcattt ttcaaaacat caactatctg tatttttact attccttgtt caaaagcttc
     6001 ttttaatatt cttgatattg ttggtcttga tattcccaat tgatttgcta tttcctgttg
     6061 attcaaatta tcttcgtaat aaagtgaaca acacttcatc attaatctta agtctccaat
     6121 ttttttcatt tgctttcctc caaaatcaag cttttcaatt atctttacat ttgttcaatc
     6181 attttactta atttcagtaa cattatagaa ggggttcata taaaaagtca actgtttttt
     6241 tgtataacaa aaaaaaggtg atacttttct tgtaccacca ccttcataaa tagttgaaaa
     6301 attgcttctt agtgcatatt atatattact tctaattgtt aaatatcatc tccataaatt
     6361 aaaaattaat tgtccactta taatgcttta aataacaata acttattaaa tcatttctaa
     6421 aaaaccaacc atttcatcat ttaaagtctt cttatatttt tattattcgc ttctctaaaa
     6481 attcttcaat aatcataaat atataatagt ttttataggg aacatttaca atagacctta
     6541 attaaactta agactttata agaaactttt tcattattta gtatttaatt cattaagtat
     6601 agtactatat tctttatcaa gcttataaaa agacattatg ataatttgta atattgttat
     6661 aaatataggt aggtatataa aacatacttt tattgatact atagctaaat cactttgagt
     6721 agctgatgtt cctacataac caccaattga taatactgca cctaatattg ctgaacctaa
     6781 tccatttccc actttaaacc caaaactaga tgcactattt ataagcccct cagttctata
     6841 tccagtcttc cattctccat attcaatagt atctgaaaca atagcaaaca ttgttgatgc
     6901 tattccagca cttcctattc cctttatagc acttgaaaat attactccta tatagtcttg
     6961 tccaactatt ataaacataa catttgacga tattaatatt attgaaccta ctattaatac
     7021 atttcttttt ccaaattttt ttataaaagg agcaaccata aacatagcta taatttggga
     7081 tatatttgaa accatattta tagaaccaaa taatttttca tctcccaaaa taaactttgc
     7141 ataatatacg gatgaaccac catttatcgc aaatacaata aatatgcaaa ctaaacataa
     7201 tgttatttgt atccagtact tatttctaaa taaagattta atacctattt taaatggtat
     7261 tacttcattc ctatcttgct taactgcttt tactctttcc ttggttccag taaatgttat
     7321 aaaaaatacc attatagatg ctattccaaa tacaaagaat gttttagccc atgctgataa
     7381 attatttcca aagtattcaa ctagaggtag tgtaagtcca tttattagta aggtaccaca
     7441 agtggctaat atcattctaa atatacttaa tactcctctt tcatactggt tttgagttat
     7501 aagtgaattt agagttgcat agggtacatt gattgctgta taaataacag tagaaactaa
     7561 gttatatgta ataaaaatat aaattaattt agaagtgata ccaagatttg atggaacaga
     7621 aaataataat actgctgcta tagcaaaagg aatcgccatt ctcaaaatcc aaggcctagc
     7681 cttaccgtat ttggatttag ttctatccac tataattccc attgttaagt cactaaaccc
     7741 atccagtatc ctagatacaa gcataatact acctacagct gctgcactaa catctgcata
     7801 attagtataa taaaacagca aaaatgctga catagctgag tatatgatat tgcaccccaa
     7861 atccccacaa ccataagaaa ttctttcaaa aaatgaaatc ttttcatatc taatagtagt
     7921 tgctttaaca ttaacttctg tattcataat accatactcc tttttgtaag atttgccttc
     7981 taatgtttct tatttcagag cataagattt acctttgctc tgaaaatttt agtaatgatt
     8041 ttattaatcg acatcttttg attcatctac tatatattca aaaaaatcaa aatcagcata
     8101 gtgtttatgc tttacacgat ctgcacaagt taaccctata aacgtacctg taaattctcc
     8161 atatttacag tactcatctg aaaacttagt tgtatcaaat acctttccta ttctctggta
     8221 gtttttttca tcataactcc attcaaaata tgacttacga ccttgtatat acagacgaag
     8281 atatattggt atatctttta ttggtatcct agtatttaaa aattccgtct tctctccatt
     8341 ttcaagatgt attattgata gtgcactttg acccaatgtt tcactataat actttcttaa
     8401 atttatataa ttcatattat cataatacat tatcaaaccc gcactatgct gatgtacctc
     8461 tgggtaaaac tccatcttag tggttattct tgcatatact gaagttaatt ttcttgccaa
     8521 tatgcttact ttatttaatg atgttcttga ttcttgtcct ctaatcctta catatcctgg
     8581 tctagactta acatctgcaa atgataatgg tgaaatccta ggagcatagt actgtaaacc
     8641 taattcattt gaatcaaagt catcaaaact tggtatagat gaaactaaat gttcttctag
     8701 tttactttct gatacgtatt cttttgccaa attactttca tcttccattc gaagccaatt
     8761 atcctttgtc catctcattt tttgaatagc tgtttctcta cctaaagtac accttaactc
     8821 tggtacaaat ggtctagatg ttagatgaac taaatatact tcacctgatg ttgtttctat
     8881 atagcttcca tgaccagatt tttgaagttt tgattctggg ttatagtatt taggctttag
     8941 atggtcaggg tcatgtcttt cataaaaatc gcctggaatt gaagttacaa ttggattcat
     9001 tgaatctttt tcataaggtc cccatatatt tttagccctt cccatagtta cactatgacc
     9061 atatccagtt ccaccttctg cacacattat ataatagtaa tctccacgct ttgttatatg
     9121 tggtgcttca atacaacctc tatcagttcc ccctgaccat attctctttg gatatcctac
     9181 tatttctttc ttttttgtac agtattccac taaacatata acaccaggtt tttcatatcc
     9241 ttctctagtc tcccagtcta atgatgcaat ccacttttta ccatcatcat catgaaaaat
     9301 agacgcatca aaacctgatg agtgtagata tactggttca ctccactctc cctttatatc
     9361 ttttgatgta attaaataat tatcaacatc aaaatatctt gcattcatag agttcataat
     9421 tccataaact atgtaaaata aatcttcttc ttcacaataa gtcaaacaag gtgcccaaat
     9481 tcctttagat gaaggaagct tcttcaaatc tatttttgtt tcatctgtca aaatatgagt
     9541 atataactcc caatttttta agtctttaga gtgataaaca ggtatacctg gaaaccattc
     9601 aaaagatgaa actacaagat aataatcatc tccctttcta caaatacaag gatctggatt
     9661 aaaacctgga agtatagggt ttttaatcat atcttttcct ccaattttag tttaatattc
     9721 tataggatac cattcttgag ctacacattt gcctaggtcc caactatccc atcctatcca
     9781 accttcttca ttaactgtat ctagtaataa cttataattc caatagtagt gaccacttcc
     9841 atttctccat gcgtcaagct gtgcttttgc tatcttccta tataattctc ttttatcgtc
     9901 ttttgataat ttatctatat ttgattcaat cccatttaga ggtgattgac ctccatttgt
     9961 atctatacca caagcatatg aattaaataa gctccactcc ccacatataa caggaaaata
    10021 tttttgcatt tgtaaaatat ctttagcata attttctcta atatatttga gatagctatc
    10081 aatactttgt tcacatccat ctgcttcagc taacatcaaa tattggtgtg tatctagaac
    10141 aacattttta aattcttcct ctctcataaa gtctttccat gcttttaatt caaatgcatc
    10201 atgaaacact ataacgtttt cctctctcat ataaggacgc attaccttat atgcttttat
    10261 ataaaaatca cgtaaaaact ctaatgaatt aggtttactc ccccttgcca tctccttatc
    10321 tacagcctta aaccgatttg gaacattcat tatgttccac attctttctg ttataggctc
    10381 atttaaaatt tggattccat acaattcatg cctcatccca taacgttttg caagtctttc
    10441 taatacattt aatgtaaacg ctacatattc tggattttgt gaccagctac atatacctga
    10501 tattcctcca ttatcaaatc cattttggct tcctggaact gtatgtaagt ctatcaatat
    10561 actaagccca tacttatctg cccaagcaaa tgctttatca agttctttaa cacatcctat
    10621 gaatggttca caatctccaa aaataaagta tggtactggt atacgaactg aattaaatcc
    10681 catagattta atcgttgcaa aatctctctc tgtgatgtat tcactacgat gagtcttaat
    10741 tctagattca tatgcctctc ttgaaagctg tcttggtaaa tagtattcat cttcagcttc
    10801 tgtgccttca aataaacttg aggtcatcca tttttctaaa actaaccaac ctcctaaatt
    10861 tacacctttt atatacacat aaaatcctcc cctcttatct tatttaaata ggtatatttt
    10921 gcacttataa taaaataatt tttttaagtt attttaatat acagttatta tatttgattt
    10981 ta
//
LOCUS       DAMJYC010000120        10872 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJYC010000120 DAMJYC010000000
VERSION     DAMJYC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.125"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(363..1328)
                     /locus_tag="PC472_003436"
     CDS             complement(363..1328)
                     /locus_tag="PC472_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728736.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINIMKEDISEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1558..2643)
                     /locus_tag="PC472_003437"
     CDS             complement(1558..2643)
                     /locus_tag="PC472_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2728737.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2679..3992)
                     /locus_tag="PC472_003438"
     CDS             complement(2679..3992)
                     /locus_tag="PC472_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2728738.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3993..6284)
                     /locus_tag="PC472_003439"
     CDS             complement(3993..6284)
                     /locus_tag="PC472_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2728739.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6274..7068)
                     /locus_tag="PC472_003440"
     CDS             complement(6274..7068)
                     /locus_tag="PC472_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2728740.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7107..7553)
                     /locus_tag="PC472_003441"
     CDS             complement(7107..7553)
                     /locus_tag="PC472_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2728741.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7625..9031)
                     /locus_tag="PC472_003442"
     CDS             complement(7625..9031)
                     /locus_tag="PC472_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2728742.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9049..10419)
                     /gene="hydA"
                     /locus_tag="PC472_003443"
     CDS             complement(9049..10419)
                     /gene="hydA"
                     /locus_tag="PC472_003443"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2728743.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10552..>10872)
                     /locus_tag="PC472_003444"
     CDS             complement(10552..>10872)
                     /locus_tag="PC472_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2728744.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFVGTEGYYGILKS
                     VYGK"
BASE COUNT         3174 a         2177 c         1136 g         4385 t
ORIGIN      
        1 agcaaaatag taatattatt tttatagtaa aaattttatt catattacta tttataattt
       61 ttatagagca attttagtgc caatatttgc atacatttgt atagtataaa atttttatgt
      121 tctcattgat aaaattttac ttatttcaag cgttctatta gattttatca tctaaatttt
      181 aggttttatt tttataaatt aattgtattt acataaaaaa aaagacctaa aaacaaattt
      241 ttaacggtct tttctcaatt ttttattttc atttctaaaa tttataataa atagtaagaa
      301 cactataaat tcttttatat aaaattctta gtttgtggat gataaattag tgaattaaaa
      361 tactacttta ctgattttag tttgacaata tcactccaat ttttactagt ggctaactct
      421 actaaattta agtctcttct tatttcagct atatcctctt ttatagaatt agtgtcacct
      481 tttatatatg ctatgttatg ttctatatta tcatgagtag ctttatttat ttcagaatta
      541 tgtaataaac ttcctaaaat attattctgt tctatctgta tctctttcat tgatttatct
      601 tcacattcta gactatcaat cttaatttca attccatata atttttcatt tgctatggat
      661 acctcgtcct taattccatt catatcttca ttaatgttat ttattttatt ttgcataaag
      721 tttacttctg atttaacttc ggaaacatct tctttcatta cagttatttc ttgtcttact
      781 tctgacatgt cttttttcat tacatttatc tcttgtttta cctctgatgt atcttctttc
      841 attacagtca tttcttgtct tacttcagac atatcttctt tcattatatt tatctcttgt
      901 cttacttcgg acatatcttc ttttaataca tttatctcct gttttacttc cgacatatct
      961 tctttcatta cagtcatttc ttgtcttact tcggatatat cctctttcat tatatttatc
     1021 tcttgtctta cctcggacat atcctctttc attatattta tctcttgtct tacctcggac
     1081 atatcttctt tcattacaat catttcctgt tttacttctg acatatttcc ttttaataca
     1141 tttatttcct gttttacttc cgacatatct tctttcatta cagttatttc ttgttttact
     1201 tccgacatat cttcttttaa cacatttatt tcttgtttta cttccgacat atcatttttt
     1261 atgctaacta catcttcatt tatagtatat aataactcta aaatcttctc ttgcacttgc
     1321 atcaccatcc ctattaatta ttataacatt ttacacaaat gctatacaat gttaacatat
     1381 atatcagaat tattaaaatt ttaattaaac aaaattgatt cactttatta cttcaaaact
     1441 aattaatatc taaaataact acaaataaac aaaaataagg tcaacaacaa aaaaggctta
     1501 tctctttgag atagcctttc ttatttttat ttaaaacttt gttttttata aaataatcta
     1561 tacattcagc tctgaacatg aaatttttct tccaagtgct tttggtttac aaactgatat
     1621 acaaagtcca caatatctac actcttcttc attcaattcc atcttatcat tcaattttct
     1681 cgcttgatat gcacaaactc tctcgcacat cttacattta gtacattttt cttcatcata
     1741 agtaaactca aagctaagtc tttcattaac atctcccata tgattatgta ctataccaca
     1801 agcattttct atagtattat atcctaatct attaagatta gattttaaat catcatgtat
     1861 tttagatatt acttgtgcgc cttttaaaat tactactgaa catataccaa caaagtcagc
     1921 acctgccata atcatttcta aggcatcatc tccacttaca catccaccta agccaattat
     1981 tggtatatta acttgttttt ttatttcata tattctctgt aaagttattg gcttgatagt
     2041 ttctccactt aaatatccta ttccatttcc acctaataaa ggttgccctg tttctatgtc
     2101 tattctaaat gctggtccta cagagtcaca agcagttatt gcatctgctc ctgcttcttc
     2161 taatttttta gcaaaatcgc ctatctcatc tatcatagga ggaagtttaa ctattacagg
     2221 tatatttact ctttcctttg catctttaag cattggtatc aaatctctat agtcataaga
     2281 tactaattca ataaagtcag ctcctgcatt tgcaactttc tctactaatt cgctactttc
     2341 ttcaattgta tgacctattg aagctataca gacagtccca tctcttttca tttgtggtat
     2401 ttcaaaatct atccattgct ctggctcaaa atctgtccat ttttcattat ttaaaagagc
     2461 attagctgta tttctaacca tatgtggtgc tggatttata gctctttctt ttcttatagt
     2521 cttagtcaca actgcccctg ctcctgcatc acttaatatc ttgcatccag ctgcactata
     2581 tgttaatggt cctgaaccta ttataagtgg tgattttaat tctttaccta aaaaattata
     2641 cattatctgc ttcccctttt catatatatt tacattcgtt aaaatttcca aaattcacaa
     2701 gcaacttctt tcatttcttt aacaatagct tttttatcta aatcaactaa ctgtttttct
     2761 tttaaaatat gtctaccatc tatgtataca tttgatataa attctttttt accctgaact
     2821 actatctggt caaatatatt atcaagagtc acaggagttt taaattcatc ttccataact
     2881 attatatcag ccttattacc aactgctatt ttaccagaat tttgaagtcc taatacataa
     2941 gcaccatgct ctgttgccat tctaaatact aaatttgctg gcattactga tgcatcttct
     3001 tcaactgatt tatgcattaa aaatgcacct ctcataactt caaaaaaatc atttatatat
     3061 ccatcagtac ccagtgccac ttttatgcct tttttaatca tttttggtac tggagaaaaa
     3121 cctccaccta cttcacagtt actaagaggc atatgaacca cttttacgcc tttttctttt
     3181 aatatatcta tttcttcatc atttactttt acacactgag atgctaatac agtttcatcc
     3241 aatatatcta aatcattata gtagtcaact gcttttttgc caaagtgttt ttcagcatag
     3301 tttggctcat atatactctc acataaatga aattgcattg gtgcatttaa tttttttgca
     3361 tcttctttag ctttcttaat aaatctatca gaacatgtga agcttgtatg tgtacacatt
     3421 attccattta ttaatttact attttctcta ctccacttta ttaaattaga attttcatct
     3481 aaacatctaa gtccattttc aaagcttatt ctttcacaac tttctaagga taaaatcgct
     3541 ttcatgccta tttcttctag tatttttcct tgctcaatta aagttccttc ttctgtattt
     3601 ggagcttcta gggtatcaca aaaagctacc acaccagatt ctataagttc tattgctgat
     3661 gctttagttg ttgccttcac ttcttttaat ccaattctat tttctataaa tggccaccaa
     3721 tagtcattta aaaaaccttc aaagtcagta aaatgtacat ttgccggtat tcccctagaa
     3781 agaactccat attgatgcat atgaccattg acgaatcctg gcattattat agcatctgat
     3841 ttatctatta tttcttcaat atttttatat tttatttcaa cttcttcatt tggcaatata
     3901 tcttttataa tgtcattttc tactacaaca gcagaatttg tatatatttc atcagcagaa
     3961 gatattaagt atttactttt aatagcaatc atttatttac ctccctgatt taattttttc
     4021 atggccatat aaactttctc agaagttata ggaagttctc ttatacgaac tcctgtggca
     4081 ttgtatattg catttgctat tgctggtgca ggtgtatgaa taactatctc tcctaaagat
     4141 ttagctccat atggacctgt tggctcataa ttatctacaa aatcaacaat tatgtttcct
     4201 atatcttttt ttgttggaac tttgtattgt aatagattag aagtttgaag ctttccatca
     4261 gaaccatgtc tagggtcttc atacattgca agccctatac cttgagttac accaccttca
     4321 acttgtattc tagcaagtgc aggattcata acggtaccac aatctgtaga acaagcataa
     4381 tttattactt taaactcccc tgttgtttta tctaactcta tttcagcaaa tcctgctaag
     4441 aatggtttag cactctcttg tataccataa gattcagatg aatataaaac ttcttgattt
     4501 ccaccaccaa cagattcttt tcctaattca cttaacaaaa caaacttctc tacatcttca
     4561 ctataacaaa ctctgtcttc atatagtatt aaatcatcta ttggtaaatc gagtttcttc
     4621 tgtgctactt ttaatatctt ttgttttaaa ctttcagcag attttattgt agcatttcca
     4681 gttacataag tagtacaaga agcgtatgca ccagtatcat atggacacat atctgtatct
     4741 cctgtataaa cggaaattct atcaatagat gtattcaatg cttttgcagc tatttgtgca
     4801 agtattgtgt cagaaccagt tcctaagtct gaagacccag aaaacaactt ataagttcca
     4861 tcttcatcca tcctcatatt tactattgcc atgtctatgt tggctatacc agaaccatga
     4921 gttcccacag acattccaac agccctcacc ttattgttgc caatatccct aacagggtac
     4981 ttttcatccc atccaataag ttttttacct ctaattatac attcatcaag tgcacaactt
     5041 cttattggaa aattcattat tccgccttca gtttgttttt ttataatatt ctttaacttt
     5101 atttctgttg ggtccatatt aagtttatgc gctaattcat caattgcaca atcaagtgca
     5161 aatgtacctt gtgttgctcc atatcctctt aatgctcctc ctggaactaa attagtatat
     5221 acagttctcc catcaaattt tattgcaggt acattattat atgttggaag tacattatga
     5281 cctgattcag agcaaactga aggtccatta tcaccataag cgcctgtatt gttcaatgct
     5341 ttcatatcta tagcttttat gtttccttct ttatctgccc caatttttat atcaaaaaac
     5401 atctgatgtc tagtattagt cattgcaaaa gtttcttctc tagtataaat tagttttgct
     5461 ggtctttttg tcatccaagt tacaaatgat gcatatatct ctgttacagc tatgtttttt
     5521 ccaccaaaac ctccacctat tcttggtttt attactctca cttttgaaag aggtattccc
     5581 agtgctttag aaacctgtct tctcatatga taagctgatt ggtttgcaga agttactact
     5641 aatctgccat tcgtatctat atacgtataa gctctatgag tttccatcat acaatgggct
     5701 tgtgattgag tcatataaga atgttctact attacatcac tttctttaaa ctctttttct
     5761 acatcccctt tacccatttt ttctctcgat acaatatttt ttgtagcatc caatccaaaa
     5821 tcaaatggac aaaacaaatc atcttcttca tggattaaaa ttttatttcc ttccgattct
     5881 cttgcatcca atataggagt cattacttca tattcaactt ttataagttt catagcttta
     5941 atagcagccc tttcatcttc agcggctatt atagcaactt catctcctac atatctaact
     6001 acgtcttcaa gtattaattg gtcatgaggt gatgcttctg gatatgactc tccaaccatt
     6061 gaatatctat aatttggaac atctttgtaa gtgaatatgt ttactacacc ttttaccttt
     6121 aatgcatttt ttatatctat gttttttatc ttcgcaaaag catgtggact tctaagaagc
     6181 ttaattgtta aaacatcttt atgaaaaatt aaatcttctg tatatattgg ttttccagta
     6241 acaatggctt cactatctat ttttcttacc tctttattta ccatcacact cacctccaat
     6301 actattatac attctcacta ctaaggcttt tgccatatct tttctgtatt ctttacttcc
     6361 tcttatatta cttccaaatg aaagttcttc acttgatact tcaccagctt tttctatatc
     6421 attttctaaa cttaatattt cacttgcttt ataggcaatt tttgctcttc ttggtctagc
     6481 gcctattgca attttataaa tacttccttc ttttaacatt gctccattta atactgcaaa
     6541 atcacctgta cattttctta tagaatcaaa tacacctata gcatcttttt taggtaatat
     6601 aacttctatt aatatatctt tttcaagctc actttctaaa aaatcagcta agttcataac
     6661 ccctttttta tataacttaa cctttgcatc taccactagt aatgaaggta ttaaatctga
     6721 aaatccatac ttagcaaata cgctagctcc tactctagct ccacttctaa attgaactcc
     6781 tactatatta gaaacagcgt ttgaaattac cccattacag taatttttaa taaccttatt
     6841 taattctaaa gttcttaaac ttgtgtctgc tccaattaat atgctatcat cagtttcatt
     6901 tatataatct aaagctaagt cttttaaatc tattgctgag ccaatatttt tactacctag
     6961 ttttaaaaat ccacatcctc caagtattat gttgtttttt cttgatatta gcagttcata
     7021 agcttcatct aaactttttg gaactacata ttcctttata gttatcatac tctctcccca
     7081 tatcaactta attttcactt aaaagtctaa gtgtttaaat atttatgtat tgctcttaat
     7141 tgacctttat atcctgtaca tctacaaagg ttaccattta cataatctat aacttcttct
     7201 tctgttggat tttgaagctc tcttttcatt gccaaaacca tcattataaa tccaggagca
     7261 caaaatccac actggtcagc cccttcgtct gcaagaaaat ctccaaactt ctttgcttca
     7321 tccaagacac cctcaagtgt tgtaatatgc tgaccgtcta ctctagcagt aagataatta
     7381 catgaaagca cggccttgtc atttaccatc actgtacata acccacatga gcctgtatca
     7441 cacccttttt taacacttac atatccattt tctcttaatg tatctgctaa aaaatcatct
     7501 ggtcttatac ttaattcttt ttctttatta tttatggtac atttgataat caaataaatc
     7561 acctcaaaac tcatattttt aagctaaagt tttactatac ttttctatga ataaattaca
     7621 tttattaata aaggctattc catttatgac cttccattct attttttata gttcctctta
     7681 cacaaagtct ctcagctgta tcttgagcat cttttaatgc tttctcttca ttagctgtca
     7741 atatttttga atcttccatt attatatttc catctactat aacactctct atatttttca
     7801 tactagaaga atatactaat gtagataccg gattatgcat aggtatcgct tttggtgaaa
     7861 gcattggatt aaatattagt aagtctgctt ttttacctat ctccaatgaa cctatctcat
     7921 cttccattcc aatagctctt gctccatcta tagttgccaa ctcaagaact ttttctgctg
     7981 acattgctaa tgggtcacac ttattaactt tatgttgaag tgctgttaat ttcataagtt
     8041 caagcatatc ttgagagtta ttactagcgg ctccatctac tcctaaacta actgttattc
     8101 cttttttaag catttctggt actggtgcaa cacctgaaga taagtacata ttacttgctg
     8161 tattatgaga cactttcata tcgtattttt tagtaagttc catatctttt tcagttagat
     8221 acacacaatg taccataagc acatttggtc caagtattcc taatttttct aaaacatcta
     8281 tgtcatattg cccatgcaat tcttttgctg cttctctatc aaaaggagtc tctgatatat
     8341 gtactgtaaa taaagcatca tcatattctt ttactactct ccatagcatt tcaagcattt
     8401 cttgagaatt tgaccatatt gctgcaggag ctactcctat tttgattctt ccattttctg
     8461 tattatggtg tttttcaaac aatcttctta catctttttc aaccgtttct acatcttgca
     8521 ttattccagg atgaactcca aattgagctc ctgtattcat acatcctctt ccaagtattc
     8581 ctcttattcc taattcttta taagcatcta ttattccatc acaaagacct ggcttactat
     8641 gaggatacat gtagtcaacc attgttgtta taccacttct aagtccttca atacatccaa
     8701 gcattgcagc atcataagta tcttttggct ctaaatagtt agctgctgga aatgtcatag
     8761 tttctaacca gtctttaagt accatatcat ctcccaatcc ttttaataaa gtttggaata
     8821 aatgattatg tgtatttata aaacctggga ataaaacttt acctttagca tctataattt
     8881 ttttaacatc agtatattta ctttctatct ctttagaatt tccaatatca gcaatcttgt
     8941 tatcttttac tactaatgcc ccatcaaaaa taacttctct ctctttattc acagttacta
     9001 ttatggcatt cttaataagt atatccatca ttcttatttc cccccgcatt atttagtaat
     9061 ttttctttta atatattgac ctgttggatg ttctttaaca ttacaacttt gtactacatg
     9121 atttcctctc aaaaatacat cttcaacttt acatttaacc ttaaatcctt catatggagt
     9181 ataatctaca ttttgttttt gagttttata actaattgtt tcttctttat tgatatttaa
     9241 aatgactata tctgcatcac ttcctacagc tatctcacct tttttaggat acattccaaa
     9301 tatttttgca gcattcgtag aagtaacttc cacaaactta tttacactta ttctattttc
     9361 taagacccca tatgtatata aaagtgctaa cctatgttca acaccaggtg ctccatttgg
     9421 tatcttactg aaatcatcaa ttcccaagtc tttttgcccc ttaaaattaa atgaacagtg
     9481 gtctgtgcca actgtttgaa tatctccact agacaatcct tcccaaaggt aatttatgtc
     9541 ttcttttttt cttaaaggag gagacattac atacttagca ccctcaaatt tatccttatt
     9601 atataaattg tcttctaata atagatactg tgggcatgtt tctactatca tatctatatt
     9661 tttttctcta gccattttta cagtttcaag tgactcccta caacttaagt ggactacata
     9721 ggattttgaa ttagataatt tagttatatc agcaagtctt gaaacagact ctttctcaac
     9781 tatgttaggt cttgtaagtg gatgatattt tggctctaaa ttaccatttt gtatattttc
     9841 ttctatcaat acatctagta aatccccatt ttcacaatga aatccaacta tacaacctaa
     9901 atcccttgct ttctttaaaa ccctgtatat tgttccatca tctactttca tgccatcata
     9961 tgccatatac atcttaaaag atactatccc atctgaaata agtttttcca tatgattaaa
    10021 tgtatcttta tttaaacatg taattgtcat atgaaaacca taatcacaat aacaatttcc
    10081 acttgctttt ttatgatatg cttctacacc ttgcagtagg ttttccccct cattggcttc
    10141 tgcaaaatct agtattgtag tagttcctcc agctatagct gctcttgttc ctgtttcaaa
    10201 attatctgca gttgtaatag aacctgcatt catatcaaaa tgagtatgtg tgtctattcc
    10261 accaggaatt acaattttac ctgtagcatc aataacctta tcaccattta tttctaagtt
    10321 atttcctatt tcaactatct tttcatcttc tatccttaaa tcacttatat aagtcttatc
    10381 agaggtaatt atagttccac ctcttagtat tgttcccata tatacctcct aacttttaag
    10441 ctttaaagta ttttctaagt attgtaaaac tttatttaaa attgtatttt ctaaaggtgc
    10501 tatattcagc acctctagac tatatttaaa taatttattg tgaactaaat attattttcc
    10561 gtatacagat tttaatattc catagtaacc ttctgttcct acaaataact gttcttgctc
    10621 tatatactca tctattgtgt gagcaagatt ttccttagaa ggaccaaatc ctatagtttt
    10681 tattccagct tcacctgcat agtgacttcc attcgtacag aatgagtatt gagttatttc
    10741 tgaatctatt ccagcttctt ttaatccctt atatgctgct tgaacaaatt catcttcttc
    10801 gtcatataac caacctggga agaatctttc actttctata gtatttcctg tatagcaatc
    10861 agctttttca ac
//
LOCUS       DAMJYC010000121        10787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJYC010000121 DAMJYC010000000
VERSION     DAMJYC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.126"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            186..1346
                     /locus_tag="PC472_003445"
     CDS             186..1346
                     /locus_tag="PC472_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2728745.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1688..1849
                     /locus_tag="PC472_003446"
     CDS             1688..1849
                     /locus_tag="PC472_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728746.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2042..2671
                     /locus_tag="PC472_003447"
     CDS             2042..2671
                     /locus_tag="PC472_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2728747.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2910..4169
                     /locus_tag="PC472_003448"
     CDS             2910..4169
                     /locus_tag="PC472_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2728748.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKGITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4427..5422
                     /locus_tag="PC472_003449"
     CDS             4427..5422
                     /locus_tag="PC472_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2728749.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQFKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5668..6735
                     /locus_tag="PC472_003450"
     CDS             5668..6735
                     /locus_tag="PC472_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2728750.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7269..9179
                     /locus_tag="PC472_003451"
     CDS             7269..9179
                     /locus_tag="PC472_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2728751.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEEYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9242..9865)
                     /gene="ytaF"
                     /locus_tag="PC472_003452"
     CDS             complement(9242..9865)
                     /gene="ytaF"
                     /locus_tag="PC472_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2728752.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10053..10697
                     /locus_tag="PC472_003453"
     CDS             10053..10697
                     /locus_tag="PC472_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728753.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4418 a         1061 c         1763 g         3545 t
ORIGIN      
        1 ttattcattt ctaaagtaat taatgtgcaa atctttatta atatatgtta atagttaggt
       61 atatatgtat ttaattagta tgatatagat aaaagtattt attttaattg tctattatct
      121 gaaaaagtct ttacttagaa atcataaaaa tcaaattaat taggaaaaga aagggtgtgt
      181 ataatgtgat tgagactgga tttgtatatt gtagtttctt ggttgtattc gtaggtgtaa
      241 ttttttttat ggttgataaa ttgaaatgga aggtatttga tgttattaat ccaatgcttt
      301 taatatactt aggttcagct atacttggat catttggttt atttgcacag aatgaagatg
      361 ttggaacgta tcaaaacata ataacagtga acttccttcc attaatgtta gtgtttttgt
      421 taattcaatg tgatttaaga aaaatattaa agatggggcc taaaatgtta ttatcatttt
      481 tctgtgctac atttacattt atgcttggat ttgttatagc attcataatt tttaaaaatt
      541 caatgggtgc agattcatgg aaaattcttg gggcagcatc tggttcatgg ataggtggct
      601 catcaaatat gattgcagca gctaatgctc taggagttgc agaagaagga cttagttatg
      661 caatacttat gggttctata ggatatacag tatggatgtc tgtttgtcta atgtctgtga
      721 aatttggacc taaatttaac aaatggacaa agtgcgatac aagttttata gatgaggtta
      781 catcaaaact tgaaaaagaa aataaagata atacggcacc tacgtttgta gaacttatga
      841 ttttactttc tgttgggttt ggagttgcgt cttttgcaca aattgtatcc aagatacttc
      901 caacttcagg tatacttaat gaaactatgt gggtagtact tgttgcatct acagtatcaa
      961 tattattggg tatgacaaaa atatcaaagc ttaaagggag tacactggta ggaaatgtgt
     1021 ttatgtattt actccttgca gctacaggta gtaaagcaaa tttctttgga ttgtcagaag
     1081 cacctgttta cataatgttt ggattggtag tagtccttgt acatgctatt tcatttatag
     1141 ggcttgctaa actatttaaa cttgatttat ttacttgtga agtaggaagt atagctaata
     1201 taggaggagt aactacagct ccaatgattg ctggggttca caatcctgta ttaataccag
     1261 taggtgtact tatggggctt ctaggaaata ttatagggac ttattgtgct cttatggtaa
     1321 ctcaaatatt acatatgtta gcttaataaa aatatggtat aaaattaata atttaaataa
     1381 gaaaagtcat ttcaatttaa gcagaagctt ttactgaaat ggcttttttg tttgacaaga
     1441 taatctattt caagaagatg ttttgacata tttagtgtat aatattgtaa aatttactaa
     1501 aaacgagata tatttaattt ttatattgaa tcgaacgtct gtatgatgta taataaatat
     1561 acagaacgaa tgtttgcaga acgggtgttt tttgaattta ttttaataat ttatgaatat
     1621 atatcagaat gcataaatat atttaaatat aaagattaaa aagaatttaa aggagtgttg
     1681 ataacttatg ataattccat caagaagaat acaagatata acattaacaa atttaaaaaa
     1741 tggtgaagta actttaattg aattggatga aatatacaag aagatgggat ttttatttgt
     1801 tgttagtgaa ggcaaattga aaaagataaa aaaggagaat catcattaga aatgaatgaa
     1861 ataataaata ttataatagt ttataaaacc taacttatgt agaatataaa tacttaagtt
     1921 aggttttttt gtgataaagt ttataagtac tcaattttat aagataaata atggtcttaa
     1981 gtgaaaagta aataagttta aattttagta agtattatat tgcataagag gtgatattat
     2041 tatgaaaata gtaacctaca atattcataa aggtatggat tcaaataata gattaactct
     2101 aactaaaatg gggttatatt taaaacaatt ggattgtgat gtaatttgtt tgcaagaagt
     2161 attatatcct caatttttag ctttaaaagc tgttttaaat atggatggtg tctttgcgac
     2221 aaatgtaaaa aaagtaaata tgatatatgg tatttgtaca tttacaacat ctaaaatgtt
     2281 aaacagtaat cattttttct taactagcaa aaaagaacaa cggggagctc tttgtataac
     2341 tatagatgca tatggaagaa ttataaatgt aataaatact cacttgggac ttgataggca
     2401 ggagagagca aaacaattag acgaaataat agactatagg aacagattag tggggatagt
     2461 tattttatgt ggtgatttta atgagaaaaa tgtcttttta agtatgttta atgatatggc
     2521 tatttcacta aacaagtctt acttaccaac atttgaaaaa tcaaactcta gaatagatta
     2581 catttttgtt aataaaaata ctgaactaaa aggatacaca gtagaaaaaa tttacctgtc
     2641 agaccattat cctgtaatag gttacattta aatttcaata taaatgaaat tagttgttat
     2701 aaaataaaca attttttgtt aagaaatagt aaaagaaata taataaaaaa tatcaaaaat
     2761 aaaactaatt ataagacata aatttacaat aaagttacat ttgattaaat tccttgttta
     2821 acaaaagcta ttcaaatata atctaaggta gttttgaaat gggaactcta ttatgcaaat
     2881 ttaagctagt gttaaaatga ggtgaagtta tgggcagaag aacaacaaga gtttcaagat
     2941 taggtaaaga aaaaaaacga agtgttttaa ttgtattggc agctttagtt ctaataatgg
     3001 gtggatttac ttattttttt aacagtaaat ttctgtacaa tgggaaaatc gctaaaaatg
     3061 tatatataga aggagtaaat gtatctgata tgactaaagc agaggcatta aaaggtataa
     3121 cagataagta tactccagaa gacttaaatc ttgcttatga tgggaaaaaa tatacaatat
     3181 caccaaaaga tattgattta aaatatgata cagaagatgt tgtaaaagat gcttatgaaa
     3241 gtacaaaaaa agggtcctat tttcaaaact taaaaaaata tattgatata agagtgaata
     3301 aggcaaatat gaagataaaa gctgaatatg atgaggcaaa acttagttcc aaagtgtctt
     3361 ctatagcaga tagtataaat gttaagatga aaaatgcttc tattagtgtt ggaagtggtg
     3421 gactaagtta tacagactct gttgtaggta gagaatttga tttggcagca aataaagagt
     3481 ctatatataa tatgataaaa aataaagagc ataaaacttt agaacttaaa gtaaatcttc
     3541 aaaaaccaga tataacaact gaacaagtaa aaactgtaaa ttctgttata ggtcaatatt
     3601 caactactta ttcacaagct gtagagggta ggtcatataa tgtaggatta tcagcaagaa
     3661 aaactagtga tgtattatta atgccaggtg aagaattttc atacaataaa cttacaggac
     3721 ctagtaataa agcaaatggt tacaaagatg cacctgttat agtttatggt aagctagaac
     3781 aatcagcagg aggaggtgtt tgtcaaacat cttcaacagt ttataatgct gctcttcttt
     3841 cgggtatgga gataactcaa gttacaaatc attcatctgc atctacttat gtgcctaaag
     3901 ggagagatgc tactgtgagc gatggaggat taaacttaaa gtttaaaaat ccatataagc
     3961 atcctgtata tattaaaaat tatgcaggtg gaggaagtgt atcatcagta atctatggta
     4021 attctggtga caaaccaaat atatctatag aggtaaaaca aactggtcag aataaatatt
     4081 ctacatatcg aatatttaaa gattcaagtg gaaaggtcat aaaaaaagaa catatatcaa
     4141 atagttctta taaagaatta aaaaaataga taatttttta gagaaatatt tgtaatataa
     4201 aagggagtgt ctcaaaatga actttttagt ttagagacac tctttttatg tgaaatcaaa
     4261 tatattttca tcatttcaac aatacaaaag aagcgtatct ctattttgag acagcctttt
     4321 ttgtaaaaat taacactatt agttcttgtt ttaatattac aatcattaat atatagaaaa
     4381 tattcctata tattggtata attgtaaata gatttagtta tataaaatgt atgaggaggt
     4441 gacatggttg aattttatag gcaatagata tgaggtagta aattctgatg acgttttaga
     4501 aattaacaaa atctataaag ctagagatgt tttttacagg aaaaatgtgt tgataaaagt
     4561 tataaagcac aataattata tctgtgaaga ttttgtttca aatttaattg atgaaagtac
     4621 tgctttagat gagataaact ctccttatat actaaaaata atagatgttg ggattcactg
     4681 tacagaagaa acaacattat attatgttgt aagtgaagac tttaatggta tcggactaga
     4741 tgaattaatt ctaggtaatt atcttcattt agaagcaatt gtaaacataa tgatacaagt
     4801 tttaaaagct ttagaaatga tacataggaa tcattcatat catggtagtt taaaatcgag
     4861 tagtataata gtagatacag aatacaatat aaaaatttgt gattttggaa ttacaaaagc
     4921 aaataatggg gttaatacaa gaagctatgg aaacaggaga tatttatgcc cacatcaact
     4981 atgtataaat tatactgata aagaaagtga tttttttgca acaggtctta ttctatttga
     5041 atctatattt aaaaaattgc cttttggaga atcaaattca gaagaaaaaa tgctacaatc
     5101 tattgataag ggattagatt ggaatagtat aaaagcaatt aatggaaata ctgaacttat
     5161 aaatgttata aaaaggcttc ttggaagaaa taataagtat accagagcca atgatattat
     5221 tattgattta agtaaagtaa tgtatgaaaa agcatatata gaagttgaag atgagaaaga
     5281 agaagagcag aaacaagaca aagttataca atttaaaaaa gaatataaaa gtaaaatgag
     5341 aaaaaaatta cataaaaaac ttgttgttgc cggtctagct atagtcatga tttcaatgat
     5401 tattattagt tccataatat aaaaattgta tattaagtat tttaaaatca atgtctataa
     5461 aggatataca tagaaatttt agtgtagtct taaattatga aaggaagtgg ttattatgtt
     5521 aacaaaaaat aatgaggtat taaacgtcta ggagtatttt aacaaatagc cacctgcttt
     5581 tacagtttag aataaaaaag ctgtggctaa aggtcacagc tttttgctac tttttaaagg
     5641 caggagaata ttaaaaagga gcaaattatg agcaatataa aaataatagc aagtaataaa
     5701 tgttggattg aagatatagc aaggaaacaa cttgaagata tttcaaagtt aaatggaatt
     5761 ttaagagtag taggattacc tgacttacat gcaggtaaaa tacctgtagg tttagcagtt
     5821 gagactaaaa atattattta tccacatata attggaaatg atataggatg cggaatgact
     5881 ttatttaaaa ctggggttct aaaaaagaaa tttaaaaaag acaaatggat taagtcactt
     5941 agtaagatta aagatttgtc tgatatagaa ataaaaaaca catataaaga agaatgtcca
     6001 attttaaatt taggaactat agggagtgga aatcattttg tagaaattca atgtataagt
     6061 gaaatttata ataaagaaca atttgaacaa ttaaaatttt catctgatga tattatgatg
     6121 ttagttcact gtgggtcaag aaattatgga gaagatattt taaaaaaatt ttatgataaa
     6181 gatggacttg aagttgaaag tgagaaagct atagattata ttaaaaaaca tgataatgct
     6241 ttaatgtggg cagagcgtaa tcgtgaagct atcagtaaaa aaattatgca gtcaataggt
     6301 acatctggag atgttgaaac aatattctct ataaatcata attttataga gaaaagggaa
     6361 gataaattta tacatagaaa aggtgctgtt tcaagtgaaa gaggagctgt gattatacca
     6421 ggttcaagag gcagtctatc atatatagtt atgcctactg aaaatacaga gatatcttta
     6481 tattctctat cacatggagc aggtagaaaa tggtctcgtt cagtttgtaa atctcgcctt
     6541 aaaagtaagt atagtaaaga tactataaaa caaactaaat ttaaaagtca gattatatgt
     6601 aatgatgtaa atttattatt tcaagaagct ccagaggcat ataagaatat agagcagatt
     6661 attgaaagtt tgatagaata tgaacttatt caagtagtgg caactatgaa gccattgatt
     6721 acctataaag gataattttt aaagttaaaa aagattaaaa ttatattata aatagctaat
     6781 tttatggaac atttaaaagt gttttattat tagctatttt tttattttgt agagacgata
     6841 atttatagag ctaaattctt tgatatataa tgattacaaa gttagactgt attggaaaat
     6901 gcacatacat gaattgatga caatataagt gattctaaaa tataaatgaa ggtataagct
     6961 tattttcact ataaaaattt tcactgtaca aaaacattaa tgacattatt tttattgaca
     7021 taaatatata atttatggaa taatattaga tgcactgata taagtgttta aataagaata
     7081 gaaaaggaga agtgttttat gaaaaaaaat actaacatta atgagtccgg agttaaactt
     7141 cttaataaat aaggagtttg atatataata actccttatt tattaagttg ttcgcaaaat
     7201 aactattatc aaagtaaatg atagggtgat ttaaaagacg ataacttata aataaaggag
     7261 aaaaaataat gaatagtaat acatttgaaa gattacaact aaatgaagtt aaagaattaa
     7321 taaaaataca ttgtgtaagt tcactaggaa aaaatttaat agataaatta actcctagtg
     7381 ggaatattgg agtagtaaga aggaaattag cagaaaataa agaagctaga aagataattg
     7441 aaaatagtaa tcacatacca cttgaaggat tgtttaatgc aggttctaca atagataaaa
     7501 ttgaaaaagg aatgataata gaacctgttg aacttgtaaa tattgaagat tttttaagag
     7561 gttgtaggaa gatgaaagct tttatgttag aaaaagaatt ttattcacct acattaagtt
     7621 cttatgcctt aaatataaca gaatgtaaaa gtatagaaga tgagataaat tactgtataa
     7681 aatctaataa agttgattct aatgctagta aagaattaaa aaagataaga cgaaatatag
     7741 aaattacaga aggaaagata aaagatagat taaataaatt tataacatca actgtaaata
     7801 aaaagtatat acaagagttt ataataagta aaagaaatga tcgatatgta ataccaatta
     7861 aatcttctta taaaaatgaa gttaatggta ctatattaga tacatcatca aaaggaaata
     7921 ctgtatttat tgaacctatt agtgtttcaa atttaagtac agagttaact atgctaaaag
     7981 ctgatgaaac tattgaagaa tacaaaatat tatcttattt aacagaactt atatttgaga
     8041 agataagtca gataaaatta aatatagaga tactttcaga atacgatatg gtatttgcaa
     8101 aagctaagta tagtcaaaaa ataaaaggaa ttactccaaa aataaataat aatggctata
     8161 taaaaataat taaaggtaaa catccacttc taacaggaga tgcagtacct ttagattttg
     8221 aaataggcaa aaattataga agtcttataa taacaggtcc taatgcagga ggaaaaactg
     8281 taacccttaa gactgttggt ttactaactt taatggttca atgtggattt gatatttctg
     8341 caaaagaagg tagtgaattt agtgtatttg aaaaagtttt tgtagatata ggggataatc
     8401 aaagtattga aaatgcctta agtacttttt catcacatat aaaaaatatt gcagagatta
     8461 tgagtttatc aaataattct actcttgttc tgtttgatga aataggaagt ggaactgaac
     8521 ctaatgaagg agctggttta gcaatatcat tattagaaga gttttacaaa atgggatgta
     8581 taactatagc atcaactcat tatggagaga taaaaaagtt tgctacattg catccagaat
     8641 ttgaaaatgc tggtatgatg tttgataaag aaacgttaga accattgtat aagttaacta
     8701 ttggaaaatc tgaagatagt aatgctcttt ttatttctaa aaaaatggga ataaaaaata
     8761 gagttttaga tagagctaaa gaatatgtaa ttgatagaaa ctacaattta gatttagtta
     8821 aaagaagtaa gctacaatct aatattgaag ataaaaatag ttctgaagtc attttaaact
     8881 atacagatta taatgtaggg gataaggtaa aattactaga tgaagatgat tttggaattg
     8941 tatataaacc tatggacaag tttaataatg tagaagtatt gtttaatgat aattttattt
     9001 cagtaaatat aaaaagatta caacttagca taaaagcatc agaattgtat ccagaagagt
     9061 atgatttaga tgtattattt actagtttta aagaaagaaa attagaaaaa gatattcaaa
     9121 gaggttcaaa aaaagctctt aaaaagattc agaaagaaat tagagagaat agaaaataaa
     9181 tataaaaagc acaaaagaaa gcaatctaaa gagcaaagtt tatctttaga ttgctttctt
     9241 attaacatat cttcataaat ccaatgacta acaagattaa accacttaac caagataagt
     9301 ttaaatttac gtttttaaca aacatatttc ctagtaaaca accaagtaat actgcaatta
     9361 tatttgatat taaagaaaat agaataactt gtatataatt cacacctaca aggccactac
     9421 caaaaccgat agctaatcca tctaaactta aagcaagtgc aagtgtaaat gcttcagcag
     9481 atttaagcac tttagattta cttgtatcta agacatcagg ttcgatactt acattaaaat
     9541 ttaatttaag gtctaacata ttaaaattat aattctttga gtgtatagat tttttcttaa
     9601 aataagattt aaaaaaactt tcaattagtc tagcacttcc taataaaaat aaaataaaga
     9661 aacatattaa cattgttata ttttgaggta aaaagtctct aactattgta cctgcaaaaa
     9721 gcgatattgc taaaatagct gaacaaacta cgctgataat tatatttgaa tataagggaa
     9781 ctttaacttt atttacgcca tatccaaagc ttgtcacaaa ggcatctata caaagagaag
     9841 taactaataa aattgattca atcacagtag attaacatcc tttctcttaa attttgtact
     9901 tatattagtt atagtattgt aaaaatggat aaattgttac agataatcca ataaattgtt
     9961 gataaaattc caacatcata caacacctaa ataacaaaag aatcatatca tataataata
    10021 taaaaccaat cataataaag cgggtgaaaa taatggaaca aaataataag gttgggaatt
    10081 tcggtgattt gttgaagatt ttattagatg aaaaagaatt atcaatggga gaactaagta
    10141 aaaaaagtgg aattgataaa tctactatat ctcgtatagc taataataaa caaaagccaa
    10201 atataaatca tttagaaaag atggcaatac atttaaatat taacctagaa gaactgttga
    10261 aagcttcagg atatgaattt agaaacagca ataatcaaat atttaatgca gatagtgatt
    10321 ttagtaactt tgatgatatt cttggatttg caaatttaat caatgataag aactttaatg
    10381 gaaatattga aaaagagttg agtaagtgta agttatatgt ccaaacagat gaaggtaaaa
    10441 aattactttt tgataatttt aataaaaaaa tagattctat tgaaaaacaa ggtcaattta
    10501 cagacagatt aagacaaatg tatgctgatt tttgtacaga tggacttaca ataaagaaat
    10561 atttattgat tggaagtatg ctactttact ttgttatatc aacagatgtg attccagact
    10621 tcgtgttccc aataggattt atggatgatt tagtagcatt aaatatagtt acaaaattac
    10681 ttaaaaatga taagtaagtt ttagaatcat atttaatata taaataaatc aaagtatata
    10741 aaatttatta ttttttatat gaaacaaatt aaaagcacaa tgaagta
//
LOCUS       DAMJYC010000122        10581 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJYC010000122 DAMJYC010000000
VERSION     DAMJYC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.127"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(123..692)
                     /locus_tag="PC472_003454"
     CDS             complement(123..692)
                     /locus_tag="PC472_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2728754.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            895..1122
                     /locus_tag="PC472_003455"
     CDS             895..1122
                     /locus_tag="PC472_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2728755.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1341..2084)
                     /locus_tag="PC472_003456"
     CDS             complement(1341..2084)
                     /locus_tag="PC472_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2728756.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2252..3124
                     /locus_tag="PC472_003457"
     CDS             2252..3124
                     /locus_tag="PC472_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2728757.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3124..3789
                     /locus_tag="PC472_003458"
     CDS             3124..3789
                     /locus_tag="PC472_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2728758.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTLKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3810..4667
                     /locus_tag="PC472_003459"
     CDS             3810..4667
                     /locus_tag="PC472_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728759.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4823..7369)
                     /locus_tag="PC472_003460"
     CDS             complement(4823..7369)
                     /locus_tag="PC472_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2728760.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7369..8055)
                     /locus_tag="PC472_003461"
     CDS             complement(7369..8055)
                     /locus_tag="PC472_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728761.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8138..9127)
                     /locus_tag="PC472_003462"
     CDS             complement(8138..9127)
                     /locus_tag="PC472_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728762.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSNTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9114..9800)
                     /locus_tag="PC472_003463"
     CDS             complement(9114..9800)
                     /locus_tag="PC472_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728763.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10300..>10581)
                     /locus_tag="PC472_003464"
     CDS             complement(10300..>10581)
                     /locus_tag="PC472_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728764.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3725 a         1748 c         1546 g         3562 t
ORIGIN      
        1 attaaaaata ctctataaaa gctaaaaagt ttttctattc gctacatcta aaaataatta
       61 gatagaggta taaatgtatt tagtttattt atacctctag tcttttataa attatatctc
      121 tactaaattg attttttagc acgaataagt gtgctcttgg agataccagt catcttcaca
      181 acctctctat atggcttaga atttaataac cccaatgcat attgtatctg ttgattgctg
      241 tatttctttg gcctaccatc tttaaagtcc tttttctttt tagcaaattc ttttcctaat
      301 tgagtacgtt ctattatcat atttctctca agttctgcaa ctgcaagtaa tgtagtcaaa
      361 aagaatttac ccattgaagt attttctaaa aggcctatat ttaaaatatg aacacttact
      421 tctttagcaa ataaaccttc tattagttgt attccttctg atgtacttct agctagtcta
      481 tccagcttag ttgcaacaag taagtctccc ttttttagag atgatataat tttttcaaat
      541 ttaggcctgt ttgtagtagt gcctgtataa gtttccttta ttattttagc atcattatat
      601 ttattcaaaa tttcttgctg ttgctgatac aaactgttgt tgtctgtttg attttttgta
      661 cttacacggc aatatccata tataataatc atcacttcct aacataaaga ttttgtacag
      721 aagttaccca gaagcttgtt tttacttatc tttatccatt ttactaaaac gatttttggg
      781 tgtcaatact gttaactttt gacaccataa taattccttt ataatttgtt tgactatgtc
      841 aaaaattacc aatataatga aattaatgag tttatacaaa ttggaggaaa taatatgaat
      901 agtctgttaa aagaagcaat agatgaatta aaaaacatcc attgcgggga agttttttta
      961 ttaaaagaat tgttcaaagg ctatgaatgg aacaggttgg agatgaatat cagattgaaa
     1021 ttaggaaaac tttttttgta tgaagtggta actaatccca atttaaatgt agaggtcatc
     1081 aataaaaatt ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa
     1141 tagtataatc ttcttattga ttcaaaagtt aaaactttta atacaaaaca atcattctat
     1201 tttaacattt atcaatatgt ctaaatttat ataattaaca catacgaata gtattgttta
     1261 aatcacatta tttaagatag ataaatctta accctactcc agcataaata ctaaaatagg
     1321 gcttagaatt atctatattt ttatcccata aaatgcttag ctcttcctct tgattgaaca
     1381 tatatatctg gggaaataca atagaagcgt aaatttataa aatttccaaa gtcagaaaac
     1441 ttcagttcaa acttaggtat accaaaactt tcaggtgcta tttcgtcaac gccaaaatag
     1501 atgataattc cattatctgt aagataaaat gcttggtctt caggtataac taactcaaca
     1561 ttagaataat acatttctct attttcattt atttgagatt taatgtaatc agtaattata
     1621 ttaatataat caactccatc tctaaaaatg tcattaagta aaagttcttt tccagttgtc
     1681 aaatctatat tgtaattatt caatgtattg tatttaggcc catagttatt agcaaacccc
     1741 ataaggttta atatcatact cagtacataa tttttattaa aagtaactgc gtaactggta
     1801 gatacccaat agctttgttt tggaagtgaa ttttgagaag ctgcgttatt atattctcgt
     1861 tcttcttcct gtaagccatt tttaaaatta tttacagtca ttcttacagc attgtttatg
     1921 ttatttaaaa taactgggtt tacatacgtg gattgatttc taatattgta tataacagga
     1981 tactttacat catatgaaaa atacttactt ctttcaaaat cttcattaaa ttgtacatat
     2041 aatatattat atgtacaggt atttgtagat tgataatttt ccattaaaat cctccctctt
     2101 ttataaaaaa atcagataca atataatatg cttaaaacag taataatgtt aatggtaaat
     2161 gtaaagaata gaaagtatct aaaacagcaa aaaatgacta attttgtggt ataatacata
     2221 ggattgagag ttattttata ggaggaaaaa tatgttatta ttagcagata aatggaaaga
     2281 ttatgaactt atagatatgg gaaatggaga aaaattagaa cgttggggaa gttatgtact
     2341 tagaagacca gacccacagg ttgtatggcc aatggaatct gaatgggctt tatggaaaaa
     2401 ccctcatgga cattatcaca gaagcaataa aggtggaggt caatgggaac acaaaaaaag
     2461 atatccagaa caatggacta taaattataa aaacttaaag tttcacatta aaccaactgg
     2521 ctttaaacat actggattat tcccagagca agcagctaac tgggattgga tgatagagaa
     2581 aataaagaaa gctaacagac ctataaaagt gttgaatctt tttgcatata caggtggagc
     2641 tactgtagca tgtgcatctg ctggtgcaga agtatgtcat gttgatgctg caaaaggtat
     2701 ggttaattgg gcaaaagaaa atatagaatt atcaggtctt aaaaatcaaa cagtaagatt
     2761 tatagtggat gatgttgtaa agtttgttga aagagaaata agaagaggta aaaaatatga
     2821 tgcaataata atggaccctc cttcttatgg aagaggtcct aaaggagaag tatggcaaat
     2881 agaagaaaag ttatacagtt ttgtagattt atgtatggga gttttatcag ataacccttt
     2941 atttttctta attaattctt atacaacagg attttctcca atagttcttg aaaatgtatt
     3001 gtctacaaca ataggtaaaa agacaaatac aggagatata tacggtgggg aattaggaat
     3061 accagcttcc agagatggta agatacttcc ttgtggtata tttggaagat gggagaaaaa
     3121 ataatgataa aagtcatata tgaagataac catttattag ttgtagaaaa accagttaac
     3181 atattgtctc aaggagataa tactaatgat aaagatatgg taaatctttt aaaagactat
     3241 attaaaaaaa agtataacaa acctggaaat gtatatattg gtttaataca tagattggat
     3301 agaccagtag gtggagttat ggtgtttgca aaaacatcaa aagcagcatc tagattgtct
     3361 gagcaggtaa gaagtaagac tttgaaaaaa acttatctag ctgttgttca tggcaagata
     3421 aaatctaact cagatatact aaaagatttt ttatataaaa ataaaaaaac taatatggta
     3481 actgtagtga aaaaaagcca taaagatgcc aaagaagctg aacttaacta taatgtatta
     3541 gattttaaag aaaatctaag tttagttcaa gttaatttaa agactggtcg ttctcatcaa
     3601 ataagagttc aatttgcaag cagaaaacat ccattatacg gagaccaaag atatggcaaa
     3661 gaaataaata agattggaca acaaatatct ctttggtcca ataagataga aatttgccat
     3721 cctactacaa aagaaaaaat ggaatttaca tgtcatccac ctgacaaata tccatggaat
     3781 atattttaaa tctcaaatga ggtgattata tgagtaaaaa attttcaata ggagaaatgt
     3841 ctaaacttca taaaataagc atacagacac taaggtacta tgaccaaata gatttattta
     3901 aacctgttca tgttgaccaa gaaagcaatt acagatatta ctcaattgac caattttcac
     3961 aattagatat tataaaatat ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa
     4021 agcttgatga aaataatgaa gaaattctaa aattactaac aaataaaatt aatatagtgg
     4081 atgaaaaaat taaagaatta gagcttataa agaaggtact aagcacaaag aaagaaacta
     4141 tggaaaaagg tatgaaagta gatgaagttg gtaaaatagt gagaaaacat attccacgaa
     4201 gaagtatctt atctatagag tatgaagaag gattaagtag tgaagaaact atggaacttt
     4261 ccagaagaca aatagcaaat atattagaag ataatatatc tgtgttctat ggtggagtaa
     4321 gtggacttgt atctatagat aaatttttaa aagaagataa agttatttat gacaatagtt
     4381 ttgttactgt tgaaagagac ttatttgata taaaatcaca aaaacatata acagaaattt
     4441 catctggtga ttttatttgt atgacatata aagggcctta caaagaaaat tataccgctt
     4501 ttaagaaact ggttgattat gttgaatata aaagaatacc tattgaaaat tatatatatg
     4561 aaatacctat aatagaccct ctgtctacaa acaacgaaga ggaacttcta acagaaattc
     4621 aaatacaagt taaaaagaga gaaattgact taagatgtct agtgtagtaa tctagtttac
     4681 ttacacatct taatatagtt gtctattatc aacatatgat ttttctagat agtgattcct
     4741 aacagtgaga ttttcaaaaa ataggaggaa atatggctaa tgctaattcc ctcctatttt
     4801 taattaatcc atatcatttt aattaagcca tgtcatcacg aagtgcatca ataatatttt
     4861 ctttttttat cttattaata gtatacaaca ttgtaataaa tactataaat aaaacactaa
     4921 atatactaat tccaatacta aaccatggaa tcacaaaatc aatatcatct gtaaccatta
     4981 ctttatgaat tagccatgaa gaaattactg ctataggaag tccaaagaac aatgctctca
     5041 tgccataaaa ggcacactca aaattcatca tcttttggaa atcatgctca gacattccta
     5101 cagagcgaag catggcaagt tctctacgac gtaacttaat attagttgaa attgtattaa
     5161 acacattggc aatcgaaatc agagaaatca taattatgaa agtataagca aaaacattgg
     5221 caataaaaat gtaattacgg ttctcatcca tcattccata catattgtac agattgtact
     5281 cattcgtaac tccctcactc tcaataattt gctccatttc tgcaactgac tgagtaggat
     5341 ttttcgattg aaagcttaaa ctttcaattc ctgtagaaac accaggaatc tcaaacttat
     5401 ctttggctga ataaggagcc aatactctga agcttgcttt cttcacatct gaacttctga
     5461 ttcctggagg agaatcaggt ggcactactt tgacaaatga aatattcaca ttttgtctct
     5521 caggtatctc caacttgcca ttagtttcag gagtgaggct aacattcata gaagaactgt
     5581 tgaaaatatc aataatatta ttatcctttg ttaccataga aacagcaatc agttttgcat
     5641 tttgtccagt atattcttct gctggtaatc caagactttt aataatattc agataagttc
     5701 tatcatcaag aaattgcata tctactggta attcaagcat ttcactcata gaatgtgaac
     5761 cctcatagtt caaataatcg tctgtaagct cacttacttt ggcaacacat gaataggtca
     5821 taagtttctg atacaaacct tcgtaaacac catttgcagt ttttagctta tcgtaaagct
     5881 tcaacatttc gttgtctttc ataccttgtg tagaaaaaat aatatcctgg tcagtaacct
     5941 ctgacctctg atttgacatc tgttttaggt ctgctactaa agcacttgta gatataaata
     6001 aaactatgct taaaacaagc gacaatacaa tacttcgata acgtttcttg tttcttttaa
     6061 agtttttcat agcaagaact ccctcaagac cataaactcg ctgtgttagt tttgatgtct
     6121 tcacagattt agattcaatt tttacctcat ttgtctgacg aatacactcc atcactggtg
     6181 tgttggcagc ctttcttgct ggtatgtaag cagaaattaa gattgtaacc atacttacca
     6241 ctgctgcaac gatgattgca gggatagaca atgttaatgt caacggaacg tttacataga
     6301 gaatatttgc aaagttttta gctatgacag aaatcacaag tccaatactg actataccga
     6361 ttgcaacacc aattggtata ccaatcgcac caatgcaaat tccttcaaat agcaccgaat
     6421 ttcgtaactg ttttgctgta gctccaacag atgaaagtat tccaaattga tgtgttcgct
     6481 cgttcaatga catgttaaat gaattatata ttagaaaaat tgagcctagc atgattatgg
     6541 caagcacaat gccaccaacc gagtacaaaa acatattgaa caaattgtta tcagaaacac
     6601 ctatgaagcg caacacattg tcattgaaaa tggaagcatg gtctttagtg ctacttatgt
     6661 aagagcgaat ctgatgtgga tttttcaatg tgacaaaaag gctaagactg tcagtcttga
     6721 cttgtttatc ttctttggtt atcaaggtat atcctggtgc agaagattct tcaaatacgg
     6781 gctttctaca gatacccaca actgtataag ttttcttatc ctgtggcaca aaaacttcac
     6841 tcccagcagt gtatgggtca ctctgaccaa gcttttcatt ttcactcatt cgtttcccta
     6901 catccaaaga aatagtatca cctactgcaa atttgacacc accatctgtc gcaactttac
     6961 ttgaaacaag aatctctcta ctattttcag gtaatcttcc agaaattaaa gttataggca
     7021 aagtatcaaa agcttctttg ctaaatccag caataaaaag atatggttta tctggatttc
     7081 tgttaccttc aagtgttgca tagccaatat tttcaaatgt tgcagtattt gcaacttcat
     7141 cattgactct ttgttcctgt acaaaagaag aatctacatc caaaaactgg acatgccaat
     7201 caccatattt tataatagct ccatttgtca tatagttcat caaagaaact ccaaaggtag
     7261 taactgctgt aatcatagca gctgacagaa caactcctat aattgttaca agagttcgag
     7321 tgcgattctt tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt aacgattcac
     7381 cttcctctca tcacgcacta cttttccatc tgaaatacag ataattctat ctgcctgtaa
     7441 agcaatgttt tcatcatgtg tgacaataat gagagtttgg tgatatttac tatggctctc
     7501 tttcagtaac tggataatct cttgtccatt tttactgtct aagctacctg taggctcatc
     7561 agcaagcata actgctggtg cattcatcaa tgcacgacca atggcaacac gttgctgttg
     7621 gccaccagaa agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa gcaattctaa
     7681 caagtcattt aaccgttctt gattgacctt acgcttatcc ataagtatag gcaatgtgat
     7741 attttctact acatttaatg tgggtatcag attgtgaaac tgataaatca atccaacttg
     7801 tcgcctacga aaaatagcaa gtttttcatt gctttggcca tatacatctt gcccctccag
     7861 atatatcttt ccacttgttg gtacgtctac tcctgcaatg gaatgtagta gtgtagattt
     7921 accagaacca gaagaaccaa tgattgcagt aaactctcct ttttcaatag taagtgagac
     7981 atggtcaagt gcagtaacct tattttcatt cttaccataa accttacata aatcttcaat
     8041 ctttaaaaac gccattatat aagtctcctt tcatatttta taaatatatc ataggacttt
     8101 caacttacat ttaagtgaca tttaaatgag agattcgtca ctttggaaaa cgaataacaa
     8161 atattgcacc accttgtggg tgattttgtg cagtaattgt tcctccttgt ctcataataa
     8221 tcgtcttaca aagtgccaat cctattccat aaccagctgt gtttgaattt ttcccacgat
     8281 aaaatctgtt aaatagacat ggtaaatctt cttttttaaa gcctgctccg ttgtcatgaa
     8341 tagtaagttg agtaaacaaa aagttgtcct cacaaatgat atcaattttt ccattatcac
     8401 ccacactctc catgcagttt ttaaatatat tttgaattgc ttctgaaagc caatttaaat
     8461 cacccaaaat ccttactcct tttggtatat caatatgtaa agtgatattg tgcagttcca
     8521 tgggaatcaa aagttgatga agtgcagaac taatcaaatt attcacatca atttgttctt
     8581 tttgaaagac tacaatacct gcatctaaac gtgataattt taaaagggaa gtaatcagcc
     8641 agtccattcg tataagtaat tcctctgttt ctcgtatcaa ctcttttctc tcatcttcat
     8701 cagaagtatt ctctaacagt gacaaaataa gagttactga tgtaagagga gtgcgaagct
     8761 ggtgggctat gtcagctagt gagtcagcaa ggtgttcttt ttcttttttc agtgcgtaat
     8821 tttgctctct aatacgtaac gtcattttag ttatttcact ttgtagaata gaaagctcac
     8881 cctcatctga ttcagcaata tacagatggt tagcattatg aagcacaaga ttaatttgtt
     8941 ctgaaatctg tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat gctattccaa
     9001 aggtagtggc agaaacaata gaaagtattc cagtcataat actgattgca aatcccaggg
     9061 ctacagtgag aatagtaatt aataagaata aaatagcaaa ttttcgaaac tctctatttc
     9121 gaaacataac ttccccccaa tctataccct gtcccacgaa cagtcaaaat tatttgtgga
     9181 cttgaagggt catcttctat cttctctcgc aagcgtttga tgtacacagt caatgtgttg
     9241 tcattaacaa actcacctgc cgcatcccac aattcatcaa gtaatcttac cctcgtaata
     9301 atacttttag gattgctaat aaacaccaat aacaaacggt attctaaagc tgaaagaaaa
     9361 acctcattgc cattcttttt cacaacaccg gttgccgtat ctacataaag cccacaaatt
     9421 tcaaaagttg acccaaaacg cccacttttt cgtagtgctg ttctaattcg cgcaatcagt
     9481 tcacgtggac gaaaaggttt agtaatataa tcatctgcac ccatattcag tccagtaaca
     9541 acacttgatt catcaccgga agctgtcaaa aagataacag gaatgtcttg cttttctttg
     9601 atttctgtgc aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat aagtgccaaa
     9661 tcaaatttat tcttagcaat ctctacaagt gcttcacttc ttgtggatac atgggttact
     9721 ataaatcctt ctgaattaag caaaagaata agatttttgg caattgcttt atcatcctca
     9781 actaaaaata tccgtttcat ttactttcat cctcctttat tttattagtt ttatatattc
     9841 cagcagagca atagtacact actattttac ttatcattac acaattttca gtagtttctt
     9901 ttgttcttat ataatccatg cataatacct ccattgattt attatatttc ttataacata
     9961 aatacacaag ataagacaaa aaacataatg taagtgcaag ttttttaact cactaactta
    10021 taaacataca ctcctactaa cactactttc ttgatgacac atcttacaag cttatattca
    10081 tacatcaatg aaaaattaaa tttatccatt tattaaaata aaaggcaata gcttctatag
    10141 ctaatgccct ttatcttaaa atgatttaaa tactatacaa tttttataaa tggctataaa
    10201 taaactcgta tagaatataa aatttatcta cagtcttttg ttatacaaaa tttataacct
    10261 cttatagaac aaatttacca ctttgcaaac ttatcacttt tactcagcta tattataaac
    10321 ttttaattta tctttgttta aatgtttctt cttaacttct actaaagctt ttagcgtatc
    10381 caaagatgtc attatttcag tagaaaattc aatggctgtt cttctcatct taaatccatc
    10441 tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct
    10501 tatagcatct aatatgtttg gtctaggctc ttcaattcta tttactatat cagcctctat
    10561 tccattttct cttaaactct t
//
LOCUS       DAMJYC010000123        10548 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJYC010000123 DAMJYC010000000
VERSION     DAMJYC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.129"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            23..1723
                     /locus_tag="PC472_003465"
     CDS             23..1723
                     /locus_tag="PC472_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728765.1"
                     /translation="MKRYLKTYGVALIIVIIYSFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1893..2510
                     /locus_tag="PC472_003466"
     CDS             1893..2510
                     /locus_tag="PC472_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728766.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2871..4064
                     /locus_tag="PC472_003467"
     CDS             2871..4064
                     /locus_tag="PC472_003467"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2728767.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4082..5374
                     /gene="lysA"
                     /locus_tag="PC472_003468"
     CDS             4082..5374
                     /gene="lysA"
                     /locus_tag="PC472_003468"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2728768.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5591..6250
                     /locus_tag="PC472_003469"
     CDS             5591..6250
                     /locus_tag="PC472_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2728769.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6405..7490)
                     /locus_tag="PC472_003470"
     CDS             complement(6405..7490)
                     /locus_tag="PC472_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2728770.1"
                     /translation="MIIISRENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7681..8397
                     /locus_tag="PC472_003471"
     CDS             7681..8397
                     /locus_tag="PC472_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2728771.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8455..9033
                     /locus_tag="PC472_003472"
     CDS             8455..9033
                     /locus_tag="PC472_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2728772.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9427..10299
                     /locus_tag="PC472_003473"
     CDS             9427..10299
                     /locus_tag="PC472_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2728773.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4355 a         1116 c         1838 g         3239 t
ORIGIN      
        1 attttttaga gaggagtatt aaatgaaacg atatcttaaa acatatggag ttgcattaat
       61 aattgtaata atttattcat ttattaaatt acctgtacta agattagatt ttacatcttt
      121 attttcagta ggaataattt tctttgttgt agcaggaata ctagatatga tgttagatag
      181 aaatgaaaga gcatctaaaa tggctaaata taatttttca atagcaatag ctttgcttgt
      241 gtatattgta gtagttccct ttataacttc tacacctgta cttcatgcaa aatcatatag
      301 agaactattg ggaaaagtaa ctgaaagtaa atttactgat gacattagcc ctgtcagtgt
      361 aaatgacatt cgtcttgtag atgaagatat ggcaatgaaa ttaggtgaca aaaagcttgg
      421 agaagttcct gcaataggga gtgtatccaa attaggaaaa tttcatatac aaaatgtaga
      481 tggagagttg tactgggtag cgcctcttgt tcacagagac ataattaaat ggataacatc
      541 tctaagtggt acaagtggtt atgtaaaagt atcagctagt aatcctcaag acgtccaatt
      601 agttcaagaa atagatggaa agccaataaa gatagtttac caaccagaag cctacttaca
      661 tcaggattta caaagacatt tatacataca tggtattgta aatgttggta tgacagattt
      721 cactcttgaa ataaatgatg aaggaagacc ttattgggtt gtaagtttat atgaacataa
      781 aattggctat ggaggagcaa atgcaacagg aatagccact gttgatgcag aaactggaaa
      841 aataaatgtt tatgatgtta aaaacacacc taaatgggtg gatagaatac aaccacaaag
      901 ctttgtaaca gaccagatta aagactgggg tgtttatgtt aatggatttt taaactctgt
      961 tatatcagaa aagggagttt tagtaccaac agaaggtaca tcattagttt atggcaatga
     1021 taacaggtct tattggtata caggtataac atcttcaggt ggagatgaat ctacaattgg
     1081 atttatgtta gttgattcta gaacaaaaga agctagatta tataaacaac caggagctac
     1141 tgaaacggct gcaatgaaat cagctgaagg taatgttcaa gaaaaaaatt atgaagctac
     1201 gttccctgta atgtataata tattgggtca gccaacatat gtatcatcat taaaagataa
     1261 ggctggtctt gttaagagag ttgcatttgt atctgttgaa gattataatg tacttggaat
     1321 aggtgaagat aagaatgaag cattaagaaa ctataaggat gcattagaat ctaagggtaa
     1381 tgatttaaaa cttgataatg atttaaaaga tgaggtatta gaaggcacag tgacaagaat
     1441 aagcccagat gtgcgaggtg gtaacactaa ttattatgta actttagatt caaataaaga
     1501 tataatattt agggctactt caaaagtatc tagcgaattg ccattgactc aggttggtga
     1561 taaagtcaaa atatcgtatt caaaagaaga atctggtgta atagaaatgt cagaatttga
     1621 taacctaaat attgcaaact ttgaagataa aaaagcaaaa gaaaaaacag gaactgatga
     1681 aaatacagat tccaaagatt ctgaaaatca aggtaatgta taatttaaat aaaaagattg
     1741 ataatagtac ttaacatagt gatagctaat ttttatagaa tttaattcta taaaaattag
     1801 ctattttatt ttaattcttt gtaaatttaa tgtcataaaa ttaatataat attagtacaa
     1861 ctttgcaaat aatacttcaa ggagaatgta ctatgacgaa tatggaaaaa gagatattac
     1921 ttttaattag cagggttaga tgtgttacag agtctcaatt tagaaaaatt tatggagacc
     1981 aaaaaagata caataaaaaa aattttaaaa aaactttgag aaaaatgtgt agtgaatata
     2041 cactaaaaaa atatccttgt gatgtggatt attataatta taaagacctc tcttatgtat
     2101 actatttaaa cggtagtaaa ctttttaagg gagatgattt aataaaggct ctagtaggat
     2161 cagaattagt tgttagaatc aatactgcag gttgtgatgt aaaaagattt taccgaaatg
     2221 ttagtgtaga tgatgtgaaa tacgatttat ttattgaata tacagaccaa tttggtaata
     2281 taaagcaagt tttagttgat gttgacttaa ataatgaaat agacatttct aaatacgaaa
     2341 atctttctaa aaaaatagat aaatctacaa ttccattttt taaagtacca aacgttttgg
     2401 tagttacacc agataatcta tccgaattaa atgatttaga gagatatgat aatgagtata
     2461 gcataaattt tatagattta ggattaaata aactatttaa ttgtatataa gctaacttta
     2521 gaaaagaaaa ctatcgattt gccacatttt gtaaaataat tgttgcaata ttatatagaa
     2581 tatgataaaa ctatattaat aaattaatta aaaataatga gcctagggta gaggtagctg
     2641 tattcaatag tattcaatag gagctggtaa gcgttgataa ttgatgaaag ggtatatagc
     2701 cgaagaaatt ttgttgacgg acaaattttc tggacttatg cagaatatgc ataaggctgt
     2761 cacttttaaa gtggtgagct acaaggttac ttggtctatg aaatttacac gtccaaagta
     2821 tacctttggg cgtttttatt tatcaaaatt tagcttgagg gggagaaagc atgagtataa
     2881 ttgttcaaaa atatggtgga agttcagtag cagatactga aaaaataaaa tcaatagcag
     2941 aaaatataat agaacgaaga aaagaaaatc cacaaatggt aatagttgtg tctgccatgg
     3001 gaaaatccac tgatgaatat ataactttag caaaggaatt gagtaatgaa cctagtaaga
     3061 gggaattaga tgctttaatg tctactggtg aaatgatttc agcatcttta ttatcaatag
     3121 cattaaatgc acttggatgt aaagctataa gttataatgc ttatcagtta aatataaaaa
     3181 cgagtggtct tcatggaaag tctcaaattg atgatataaa cgtaaacaga attaaaaata
     3241 gtttagatga aggaaatgtc gtgatagtaa ctggatttca gggaatcaac gaagatggtg
     3301 atgtaaccac tcttggaaga ggtggttcag atacatctgc tgtagctctt gcggttaaat
     3361 taaatggaaa atgtgagata tatacagatg ttgatggaat ttattttaca gacccaagaa
     3421 aatattcaaa ggctagtaaa cttgatgaga ttgaatatga agaaatgctt gagttagcga
     3481 gtttaggagc acaagtaatg cattcaagaa gtatagagct tgcacaaaaa tatggtgttg
     3541 aaatctatgt cggacgtaca tgtggtacag aaaaaggaac atatataaga gggggaaaag
     3601 acatgaaatt agaggataaa gtaataacag gattagctac tagtgatgat gacagttcta
     3661 taacaataaa agattttaaa gctgaaaata tatctagctt atttgaagat atagcaacta
     3721 taggtataag tgtagatatg ataagccaaa ctgcacctat tttagataag ataagtgtat
     3781 catttacagt tccaaaagaa gagttgggag aatgtaaaaa gatagtatct aaatatacag
     3841 atgaagaaca tgtagtgatt gataataata tcactaaatt ttctttagta ggtcttggta
     3901 tgaaaaatac ttctggagtt gctgctaaag tatttaagat attcaatgaa aatggtataa
     3961 tgataaaact tataactaca tcagagattc gtataacttg tgctataaat tctgatgata
     4021 agcaagtagc tatagaaaaa atagcagaag tatttaatat ataaaatttg gggaggctat
     4081 tatgaaactt catggaacta tgactgtaaa taatgatgaa ttatatattg gaggagtgag
     4141 ttgtttagaa cttagtaaaa aatatcaaac tccactttat gtatttgatg aagctttagt
     4201 tagacaaaat tgtagagaat atatagaata ttttaaggct agagagggaa aaaataaggt
     4261 tgcttatgca gggaaagctt ttttacctct ttacatgtgt aatcttataa atgaagaaca
     4321 aatgtgttta gatgtagttt caggaggcga gttatataca gcttataaat caaattttcc
     4381 tatggaaaaa gttttctttc atggaaacaa taagactaaa gaagaaataa acatgggatt
     4441 agatttaggt gtaggtaaat ttgtagtgga taatttttat gaattagatt tgcttgaagc
     4501 attatgtgaa gataaaggta aaactcaaga gatatacttt agaataactc ctggtataga
     4561 agctcataca catgattata taaaaactgg acagatagat tctaaatttg gatttgcttt
     4621 agtaaatgga gatttattta gagcaataga aaaattaagt gaatataaaa atattaaact
     4681 tgtaggtctt cacgctcata taggttcaca aatatttgat gtagaaccat acatagatac
     4741 agtggatata atgatgaact tggtaaaaga aataaaagaa aaattcaaca tacaactaac
     4801 agaaattgac ctaggtggag gagttggcgt ttattatagc gaaggagata aacctaaaac
     4861 tataaaagag ttttgtgaaa ccataatcac taaagcagaa acttcatgta aagagcttgg
     4921 aatagaagta cctacattgg taatagaacc aggaagatca atagttgcaa atgcaggaag
     4981 tacattgtat acagtaggtt ctataaaaga tataaaagat gttagggttt atgtaagtgt
     5041 agatggaggt atgacagata atgtaagacc ttcgttgtac caagctagtt atgagtgtag
     5101 tattgtgaat aaaattaatc atgaggctat gaatcatgta acaatagctg gaaaatgttg
     5161 tgagagtgga gatatattaa ttggagatat aaacataatg gatattaaaa gtggagatat
     5221 acttataaca acatcaactg gtgcatatgg atattcaatg tcttcaaatt ataataaaat
     5281 accaagagca gcagtggtat cagtatttga tggaaatgat aagataattt gtaagagaca
     5341 aagctatgag gacttattag ctttagagat ataaaataga gaagtatata ataaaaatta
     5401 aatattaaag tttattatga gcagatttgc taggatatgt atctctaaat taatttttgg
     5461 tttattgtat cgttcgtagc atatctgctt attttttgtt ttactaacaa atttcttgta
     5521 ataaatataa aatattttaa aaactttata attatgataa aatttaaagt atatgacaag
     5581 gagggattta atgagaggaa gagtaatttt attaatatcg atattatcta tatttcttgt
     5641 aggatgttca tttaataaaa aagatgaaat aaatttagta gagcaaggaa aaacatattt
     5701 agaaaaacat aactataaaa aagctatgga atcattatcc tctgcactgg aagaagatag
     5761 tactaatgag aatgcaagag ctatgtatat gcaagctatg agaatgtcaa atatgacgga
     5821 gtttgaagaa ttaaaaaatt atgagggtgc aatcaaggaa cttaaactta tagagaaaat
     5881 caaaaatggt tcctctgtta ttaaagaaga ggcaactaaa aagaaagaag agttaactaa
     5941 attagaaaaa gaacaaaacg aagcagagga acaaagaaag aaaaaggcaa aagatacggc
     6001 tggagaagat aggtatagag ttgaatcaga tgcaaggaaa tcttcttata gtggaagtag
     6061 taaaaataat aaatctagtt ctaaaaataa tgataaaaaa tcagatagta ataagtctga
     6121 caataataac agttcttctc aaaatcaaga aaagccaaat aatagtaatc caacaccagc
     6181 accaactcca caagatacta ctggtggttc agatagtggt ggaaattctc aaaactcaaa
     6241 taatcaataa gtataaaata tatgcataaa tttaatgaac aaattatata ttataaaaaa
     6301 tatctcttag caattttttc ttactaagag atattttttg agaaataatt taataaatag
     6361 cagttaaatt ttaaactaaa aattaaagtc acagtagaga taagctattg taaacgatta
     6421 tctatatatt tatcaacata agtttttaca acagcagcta ttggtactga aagaaaaact
     6481 cctatgaaac caaatagaga ttgtcccacc aaaatagcag atattaccca tatagctcct
     6541 ataccaactt gattacccat aacttttggt tctataaaat ttccatctaa ttgttgcaat
     6601 ataaatagaa atgcaataac ccatatagtt ttttgtggac tatataatat agtcatggct
     6661 attggaggga ttgcaccaat aaaaggacca aaatagggaa ttatattacc taagaatatt
     6721 attgaagcta aaaataaagc attttctatt ctgataacaa attgaaatcc taaaaatgca
     6781 attataccaa taataagaga gtctaatatt ctaccaagta aataatcata gaaaatatca
     6841 tgagtagtcc taaaaaattc tataatgtta tctgccttgt ttctagcagt agaagaatat
     6901 agaaatctct taaatcccaa tgcaattttt tctttgtcat atataatgta tatagatagt
     6961 ataaatccca ttgtcatatt aaatagcata gaagatatac taagtgtata gattaagaag
     7021 tttttaggaa ttttaccaac aaaattactt gcttctttta ataaattatc taaactatgt
     7081 tgtatatgag gaattaaagt ttttagaaaa tctatgtttg acatattttt ttctataaaa
     7141 ctatctatat aatcagtata cataggcatt tcatttatca atctatttaa tgtattagct
     7201 attgatggaa ttaataacct aatggcaaag gctaaaataa aacctataaa tatataagat
     7261 aaaaaaatgg ctaaaagtct atgtgctttg aattttacct caaaaagcat cacaagtgga
     7321 tttaaaagta aagcaagtaa aatagctaat aaaaatgggc taaaaaactt taagaatcct
     7381 tcaatactag atataaagtc tgatggagta ttaataaatt taaaaaataa aattccaata
     7441 aacacgacta ttaaatagta ttttatattt tcccttgaaa taataatcac ttccttaaaa
     7501 tagtgtttag tcagctatta catattaata gtatgtgaaa agaaattaca attatttagg
     7561 aaaattaaat aatttttttg cttgttttta attaaggtct agtatagtat aataatgtag
     7621 caaaattaac attgataaaa atattaaaca cacataattt aagataagga aggattaagt
     7681 atgagaatta ataagtatat agcttcttgt ggaatagcat caagaagaaa agcagaagaa
     7741 ataattcttg gaggaagaat aaaggtaaat ggaagtgtag taaaagagtt atcttttaat
     7801 attgatgaag aaaaagacat agtagaattt gataataaaa aagtaaaacc aagcgaaaat
     7861 tatatatata tagtattaaa taaaccagag ggatatataa ctacagtaaa agaccaattc
     7921 aatagaccca gtgtaataga catacttaaa gatgtaaggg aaagagtata tccaataggg
     7981 agattagatt atgaaactag tggattatta atacttacta atgatggcga tttaacatat
     8041 aagcttaccc atccaaagca tgaaattgat aaaacgtatg tggcaagtgt gaagggaatt
     8101 ataagtgatg atgagataag acaattcgaa acaggactaa aaatagaaga ttacacaacg
     8161 gcacctgcca aaataagggt aactaaagaa aataaagaaa aaaattattc agtgtgtgaa
     8221 ataactatac atgagggtag aaatagacaa gtaagaaaaa tgtgcaaagc tataaatcat
     8281 ccagttttaa atttaagaag gatttctgtg gggaaaatag tattaaaaga caccaaagtt
     8341 ggagaatata gatacctaac agaagatgaa ataaaatatt taaaatcaat aaaataagat
     8401 aaaaataaat tgagaaaaaa tgaaataaag ttaatagtag ggagaaatag tattatggca
     8461 tatgcaaagt atttaactaa aattacagat ataaataaat tttatttaga aaatattata
     8521 aatgaaggtg acatagtaat tgatgctact atgggaaatg ggtatgatac aaggtatcta
     8581 gctgagaagg ttggagaaaa gggctttgta tatgcttttg atatacaaga agaagcaata
     8641 aagtctacaa gaaagaaact agagaaagaa ggatatacag atagagtaaa attgatattt
     8701 gatgggcatg aaaaaatgaa tcaatatata aaagaagaag tttcatgtgt tttatttaat
     8761 ttgggttatc ttccaagagc gaaacattca attataacca aaccagatac aactttaaag
     8821 gctataaagt taagtttaga aatgctaaaa gaaaatggag taataagcat tgccatatat
     8881 acagggcatg atggaggtca agaagaaaaa gattgcatat ataaccttgt caataattta
     8941 aatcaagatg aatttaatgt tttagaatct aagtttttaa atcaagtcaa taacccacca
     9001 caattgattt taattgaaaa gaaaaaagca taataaatac taaaaaactt gacaagattg
     9061 tattaagagt taatatatat agtgtgacct attaaaaata ctttaaataa taagtataca
     9121 acagcttatt ctcaaaacaa aatatacatc taaatatgca aaataagata gataactttt
     9181 aaatgagtaa aaattgaaaa atttaaaaga aatattggat aaatattaaa aaatgcaagg
     9241 aaagatggta taataaattt aatcgaaaat actatgtgaa gtataaggta cagaaaagtt
     9301 agaattttat gagtttaaga agctatgata aaaactaaga tgaaattgag ataaaaaata
     9361 aaatttttag agaaaaagac agtgttaata aaacaaaata gtttatataa ctaaaaggag
     9421 aaaaaaatgg aagaaacaaa tgaaatgaag gacagtaaac atttgatatc aaatatacaa
     9481 tctcaatata cgagattgag taagggtcaa aaacttatag cacaatacat tttaaataat
     9541 tatgataaag ttgcatttat gactgcatgt aaattgggtg aaactgtagg agtaagtgag
     9601 tctacagttg taagatttgc aaatgcatta ggttatagtg gatatcctaa attacaagct
     9661 gcacttcaag aactcataaa aaataaacta actacagtac aaagagttga aatggcacat
     9721 gattattccg atgattttgc aatactaaac aaagttttaa aaagtgatat agataatata
     9781 aggtctacac tagaagaaat tgacgagaga gcgtttaaag aagcttctaa caaactttta
     9841 agagcgagaa aaatatatat acttggaatg aggagttctt ttgtagtagc tcaatattta
     9901 ggtttttact tagatataat acttgacaat gttcatataa ttagaatgga tatgggagat
     9961 gcttttgagc agattgtaag aataaatgaa gaagatgtaa tagttgctat tagtttccct
    10021 cgttattcta agaaatctta tcaaattgtc aattatgcta aggaaaaagg agcccatgta
    10081 atatcattaa cagatagctt atttgcgcca gtagcatcac ttgcagataa cactcttcta
    10141 gttaagagta atatggcttc atttgtagat tctctagttc cagctcttag tatttcaaat
    10201 gcccttgcta tatctgttgg gatgaaggaa aaagaagata ttaaacagca ttttgatgat
    10261 ttagagcaaa tttggaaaag atattctgtg tatgagtaaa gtttgtatat taataaggct
    10321 atgaaattga gaatcatagc cttattagta aatactcgat tatcacttaa aaaataaatt
    10381 ttcgtttaaa taatacaaat aaatatttcg agactcaaaa cataaagatg tattctgaat
    10441 acatctttac tacgtcaact aatttattaa agaaagcatt tccattcatg gagagtgctt
    10501 tttttatttc tctaaacaat atcatttcct taattaacaa ttataaaa
//
LOCUS       DAMJYC010000124        10484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJYC010000124 DAMJYC010000000
VERSION     DAMJYC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.130"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            190..1230
                     /locus_tag="PC472_003474"
     CDS             190..1230
                     /locus_tag="PC472_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2728774.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1586..2908
                     /locus_tag="PC472_003475"
     CDS             1586..2908
                     /locus_tag="PC472_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2728775.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3205..4566
                     /locus_tag="PC472_003476"
     CDS             3205..4566
                     /locus_tag="PC472_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2728776.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4639..6033
                     /locus_tag="PC472_003477"
     CDS             4639..6033
                     /locus_tag="PC472_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2728777.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6095..6565
                     /locus_tag="PC472_003478"
     CDS             6095..6565
                     /locus_tag="PC472_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2728778.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6568..8784
                     /locus_tag="PC472_003479"
     CDS             6568..8784
                     /locus_tag="PC472_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2728779.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSLDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKIFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8867..9538
                     /locus_tag="PC472_003480"
     CDS             8867..9538
                     /locus_tag="PC472_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2728780.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9901..>10484
                     /locus_tag="PC472_003481"
     CDS             9901..>10484
                     /locus_tag="PC472_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDF2728781.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDT"
BASE COUNT         3965 a         1206 c         2089 g         3224 t
ORIGIN      
        1 tttaatagag gatttttgta aaatgaagaa agtatatcta tcaaattaaa actaagtaga
       61 tatacttttt attaagtttt gttttaaaat aatattatta aattataact aagcttttct
      121 gttatgatat aattaaatag ataatgtgaa tgattattat atagaatact tatttggagt
      181 tgatatttta tggacaaaca cagtgattca gtaatgtatg gtggaatgga agtatataat
      241 atttggggaa caacaacagt agactccata attggagagt ctgatgtaat gcgtgctctt
      301 aagaatagaa tttgtaaaat tggaaatagt gattctactg tagctataac tggagaaagt
      361 ggcactggaa aagaattaat agcaagagcc attcatctcg caggaaacag agggaataaa
      421 acattcgttg ctataaactg tgctgctata ccagaacctt tactagaaag tgaacttttt
      481 ggatatacaa aaggagcttt tagtggagca gattcaaaag gtaaaattgg taagtttgaa
      541 ttggccaatg gaggggttgt atttttagat gagataggag atatgccaat tcaactacaa
      601 tcaaagttat tgagggtatt acaggaaaag aagtttacga gaataggttc aaatgaacta
      661 attgatatag atgttagaat catatcagct actaatagag atttatttga gttggttaag
      721 gaaaataaat ttagagaaga cttatactat agattgaatg taataccttt agaagtgcct
      781 ccacttagag aaagaggaga cgatatacaa atattaataa acaattttat agacaaatat
      841 tcaagaaaga tgaagaaaaa cgtgtaccat atagagccag atgtggaaca aatgttgctt
      901 aagtatcctt ggccaggaaa tattagagag ttggaaaata ctatagaact ggcattaaac
      961 cttcttgagg aagatggaat aatacataaa ggaataataa atagaaagat acttgaatat
     1021 tttaagttaa ataatataaa tataaagtta cttgaggaaa gtgactatga gataagtatg
     1081 gaacaagaca tacttacact tgaagagttt gaaagagcct atataaagaa agtgttaaag
     1141 ttttacggaa atgatactaa gtctaaaaaa ctagctgcca aaaaacttgg aatatcacta
     1201 gctacattgt acagaaaaat agatgtataa taattaggct ttttattctc aattctaaaa
     1261 tttgaaatta taaaattgat aaaaaacact tggaaaacta ttttctaagt gtttttttgt
     1321 gtaatagcat tttattatcc aaaaataaaa aaaatttaaa agaatttaaa aaaataaatg
     1381 ctttaataaa ttatcaaata ctaacaaata taataaaaat tatatatgta aaacaattat
     1441 gttaaaaaat tggcattata attgcttcat attaagttga gatgacaaaa taaaaaaaga
     1501 aaggatgttg tgtgagataa taataactgt cattaaaaaa tagttgggag tgataacctt
     1561 gagtaaatat caaaagaatg tttctatgtt tgatattgat ggaaggccac caattttaaa
     1621 gtgtttgcca ctatcaatgc aacatatact agcaatgata gttggaactg ttacagtacc
     1681 tatagtcgta ggaggagctg tgggtgttag ccctacacaa ataacacttt tagtgcaata
     1741 tgcattaata atagctggta tatcaacact tatacaacta tatccaatag gaaatattgg
     1801 ttcaagattg cctataattt ttggagttgg ttttacttat gtaccaacac ttgtagcagt
     1861 cggaggaaat tatggattag caagcatttt gggtgcacaa ttaatagggg gagtggctac
     1921 agtattaata gggatattca taaaaaagat aagaaaattt tttccgaata tagttgcagg
     1981 aacagtggtt tttacaattg gattatcttt gtatcctata gcaataaatt atatggcagg
     2041 tggtgtaaat agtcctactt atgcctctat gcaaaactgg gcagtagcgg ctataacatt
     2101 agtggtagtt ataggtctta gtcaatttgc aaaaggatat gcaaaattgg cagcaatatt
     2161 tttgggaatt atagttggat attgtgtatc tttagttctg ggtataataa attttgaccc
     2221 tattagagag gcaacttggt ttgcgatacc taaaccgctt gaatttggga tggaatttga
     2281 tatgcctgta ataatctcag tagtaattgt aagtataatc aatgctgttc aagcaattgg
     2341 agatttatca gctactacaa tgggaggcat ggataggaat atcacagata aggaattatc
     2401 aagtggagta ataggaagtg gaatttgtac tatgatagga gctttgtttg gaggtttacc
     2461 accatcatca tatagccaaa acgtggggat ggtagctatg aacaaagtaa taagtagatt
     2521 tgtgcttgga atagctggta tatttatgtt attgtcgggg tttgtgccta aatttggtgc
     2581 actaatgact actataccat attctgtttt aggaggagct acaatatcta tatttagtat
     2641 gataacaatg acaggtataa agttaattat tacagaagag ttgtcatcaa gaaatattac
     2701 cattgttggt ttggcaattg ccttaggcat gggaataaca tcagtaccag cagcccaaga
     2761 ggcatttcct tcatttgtaa agttagtatt tggagaatct ccaatagtaa ttgcaacatt
     2821 ggttgcattc gttttaaatc taataatacc aaataaaagt ctagaggatg aggctaaaga
     2881 aagagctgtt atggaagaga ctaattaaga tataataatt taaaagaatt gtttaaatta
     2941 tttagtatga gattgttaga ttatttttga tagctattca ttttaataaa aaatttaaca
     3001 aataaatata taccaggcaa ttaatgttca tttaagttga aaatcgggga gggaatattt
     3061 agaaatttgt taatttttta taaaaatagc tcagtttagc tgtgtttatc ggttgagcaa
     3121 taaattcatg gtcataaaaa tatttataga aaataattat aaaaatattt agtcaagata
     3181 tttatgtatg ggaggattta ttttatgaaa acacgtaata cttcaaaata tgatgtcgat
     3241 gggataccac cactaaagga atcaatacct ttggccttac aacatctgtt agcaatgata
     3301 gtaggaaata tggtacctgc gatattaata gcaaacgtgg taggacttaa ccaggggcaa
     3361 gcaacaatgt tgattcaggg ttctatgtta gcagcaggtt tagcaacgtt tttacagtta
     3421 tatccaatac ctttatttaa aggatttaag ttaggttcaa gattacctgt aatgatggga
     3481 atgagttatg tatttttagg agcttgtctt tcagtagcag ctaaagatgg tttagcagct
     3541 ctatttggag cacagattgc tgctggggta atcgtattct ttgtaggatt tggagttaag
     3601 aaaattagac atatattcac tccaatagtt tctggaacta taatagcatg tatgggactt
     3661 ggactattcg caacagctat aaagaattta gctggaggag aaggcacaca aacatttgga
     3721 agcccaataa acttcacagt aggagttata gttgcttttg taataataat gataaataaa
     3781 tacggtaaag gtttagtaaa aaattcttca atattaatag gaatattagt aggttatgct
     3841 atatctttag ttttaggatt ggttgatttc tcagccgttc aaggagcagc tatagtatct
     3901 ttaccaacac cagcagcttt tggattagaa tttagaccag agttaatagt aatgtttact
     3961 attatttaca taataggtat agctgatatg atgggtgcct gtacaatagc tacagtagga
     4021 gcaatggata gagaggtaac tgacgaagaa ttagcatcag ttgttttagg aaattctata
     4081 acttctataa tttcatcact atttgcagct cttccaactg gagtatttag ccaaaataca
     4141 gtaattgtat ctatgaacaa agttacaagt agatttgtaa tagcactagg ggcacttgta
     4201 ttattactag cagggatatc tccagcattg ggtgcaataa tgacaactat accatcttgt
     4261 gttgttggtg gagcaacttt agtagttttc tcatcaatag caatgtctgg tttctctatt
     4321 atgagtatgg atggattcac tgaagaaaac aatcttatag caggtgtatc tatagcaaca
     4381 agtatgggtc ttacaactgc tccacaagtt ttagaccaat tcccagaaac aattggaact
     4441 gtccttggag ggtcaagtat agtttctgga gcaataatag ctatattact tcaaacatta
     4501 tttaaattaa agtcacgtaa aagtgctgaa aacgttactt caaatcttga ggaaaatatt
     4561 ggataaatgt attagatata atatgagaaa accatatggt aaaatgttgt aatgaaagta
     4621 ttcaaagggg gatttaagat gattgatata ctaataaaaa atgcaataat tgttacagtt
     4681 aataaagaca gagatgttat atttgatgga gcaatagcta ttcaaggtga cagaatcttg
     4741 gacataggaa atacagttga tattgagcct aagtatcttg atgctaaaaa agttatagat
     4801 gctaatggaa aagtaatatt ccctggcttt ataaatactc ataatcacct gttccaagtg
     4861 ttattaaaag gtttaggtga tgatatggca cttcatgagt ggttaaatac aatgatgttt
     4921 ccatcagcaa aattcttaac agaacaagat acttatgatg ctgcaatgct tggatgtatg
     4981 gaaggtttaa aaagtggtat aactacaatg gttgattata tgcatactca caatagacca
     5041 ggacttacag atggaatagt aaaggcatat aaagatttag gtataagagg ggttgtaggt
     5101 agaggttgta tagatttagg tattcacaaa gagcttatag aagatgtgga aacggttgaa
     5161 aaagatttga gaagggtatt cgaaaaatat cataattcag aaaatggaag aataaaaatc
     5221 tgtgtagctc cttcttcaat gtgggctata tctgaagaaa tgggtaagat gctttggaat
     5281 atagttaaag aatatgattc atattttaca gttcatatat ctgaaacaga atttgcaaga
     5341 acagcaacaa aggatataca tggagaaata gatataaaat tacttgaaaa atggggtata
     5401 gtaggtccag aagttgtagc agtacattgt gtatgtataa ctgatgaaga catggaaatg
     5461 cttaaaaagt atgacataaa agtttctcat aatgtagcaa gtaacatgta tcttgcttct
     5521 ggtgttgcac ctgtgccaga aatgttaaaa aaaggtataa atgtgagttt aggtcttgac
     5581 ggtgcagcaa gtaataacgc acaagatatg gtagagctta tgaaattaac agctttacaa
     5641 cacaaagtaa ataattgtga ccctctggca atttctgcag agaaagttct tgaaatggct
     5701 acaattgagg gagcaagaac tcttagaatg gaagatgaaa taggttcttt ggagattggg
     5761 aaaaaagctg acctagtaat atttaactca atgctttctc caaaatcaat acctcttcac
     5821 aatcctgtat caactttggt atactcagct agtatgcaca atgtagagag tgttttagtt
     5881 gatggaaatg taatacttga aaatggaaaa gtaactacaa ttgaaagtga agaacaagtg
     5941 ttgcttaatg cacaacatgc agcagaagaa ttatgtaaga gagctaatgt aactaataga
     6001 atggaaggac ataagtggaa tagcttatac taatattaaa ataagattgt aataatattc
     6061 tattacaaca ttagtttatc tgtaggagga gaaaatggaa aaaataaaat taaatattaa
     6121 tgattacatg tatgaaatag aagttgaaaa gaatgaaact cttttgcatg tacttcgtga
     6181 aaaattaaat ctaactggag cgaaacaagg ttgtaatagt ggcagttgtg gagcatgtaa
     6241 agttttgata gatggagttg acatgaaatc ttgtaaactt cttgcaaaaa actgcgaagg
     6301 taaagttata tacactatag aaagttttgc taatggagaa aatcttcatc caatacaaga
     6361 agcttttata gaagtcggag ctgtacaatg tggatattgt actcctggta tgattataac
     6421 aacacaagct cttttaaata gaaatgcatc tcctaatgaa gatgaaatta gagaagcttt
     6481 taaagaaaat ctctgtagat gcacaggata tgtaaagatt atagaagctg taaaattatc
     6541 tgcagctaga ttagggggta gataattatg gaaaaggtaa taggaaaatc ataccctcta
     6601 aaagatgcag ctttaaaagt tactggtcaa atgaaatacg tggctgatat gaaaccacaa
     6661 aatgtacttt acgctaagat gcttttaagc cctgtagcac atgcaaaaat aaaaagttta
     6721 gatacaagtg aagctgagaa agtagtaggt gttagagcta tagctactta tctcaataca
     6781 tcacaaagaa aatacaatgg ttcattaaga ttttatgagc acaaaatacc tgaaaatgaa
     6841 gttatatttt cagatacagt tagatttgtt ggtgaccgtg ttgcagcagt agctgcagag
     6901 actccagaaa tagcagagaa agctgtaaaa cttataaaag ttgaatatga agaattgcca
     6961 gctgtattta ctatacaaga tgcaataaaa gaagatgcta tctgcataca tggagattca
     7021 aatatagttt ctcaaaacaa cataaatggg ggagatgtag aaaaaggctt caaggaatgt
     7081 gactatatat ttgaagatga atactctact ccagcaatac atcatagtgc aatagagact
     7141 catagtgtaa ttgcacacta tgattataat gataaattaa ctgtacatac accttgccaa
     7201 aatacatttg gatttagaat aatactatct caaatttttg ggatgtcatt aaataaagta
     7261 agagttataa gacctgctat tggaggctca tttggtggaa aattagaact tacaattgaa
     7321 ccagtagcag ccgcattatc aaaaatgaca agaagaccag ttaaaatggt tcttacaaga
     7381 aaggaatcta tgatatcgac aagaactcgt catgggtctt ttgtaaaatt aaaaactggt
     7441 gtaaataaag atggtgaagt aatagcacaa gacataaaaa tatatacaaa tacaggagca
     7501 tatgcatcaa gtgctttaaa tgttatagga gcacttagtc ataaagtatt taaagtttat
     7561 aaaataccaa atataaagtt tactgggatg cctgtatata caaatacacc aatagcagga
     7621 gctatgagag gctatgggtc acctcaaata tttatggctc aacaagctca atttgcgaaa
     7681 atagcaaagg aaataggtat ggatttagtc gattttcaaa ataaaaatgc agtagagcca
     7741 gatgatgtcg atataatctt ccacggttct cttggaaatc ctagagtatt ggattgtata
     7801 gagcaaggta agaagatatt taagtgggat gaaaagaaaa aacttccaaa agaagaagga
     7861 aaatatttaa gaggtatagg tatggcaata ggtgcacatg gtaatggagt ttttggtgca
     7921 catagagatg ttgttacatt gactcttaaa ttaaatgaag atggtacgtt gacactacta
     7981 acaggagctc atgatatggg taatggtgtt gtaactatgc aaacgatgat ggtagctgaa
     8041 gttttaggaa taacacctga taaagttgat acatttgaaa ctgatacgga tgcatgtaca
     8101 ttcaaccttg gtgactatgc aagtagaggg gtatttgtag aaggtggagg agctaaaaag
     8161 acagcagaga aattgaaaaa tcttatatta gaagaagcag aaaaattact tgaaacatca
     8221 aaagaagatt tatatctaga caatggatat gtaattagta aacttgatga aaatgtaaaa
     8281 gcatcacttt ctgatgtcgc agtatatagt caaagtaaat cattaagaga gttaactgtt
     8341 gttgaagatt attcttcacc agctggacgt acttcttatg gtgtacattt tgctgaagtt
     8401 ttagtagata aagaaactaa ggatataaaa gtagttgatt ttgtagctgt acatgatgtt
     8461 ggtagagtta taaatcctct aaatttagaa ggacaattag aaggtgggat tcatatgggt
     8521 ctaggatacg cattgagtga agaattggca tttgatgaac aaggtagacc taaagctact
     8581 gattttaaaa catataaatt atttagaact gtagatatgc caaaatgtca agttgcattt
     8641 gtagatggtt atgaaaaagc tgggcctttt ggtggtaaga gtattggaga atgtgccgtt
     8701 gtaccagttg cgccagctgt tgcaaatgca gtatataatg caactgatgt agagatacat
     8761 tcattaccaa taaaattaaa atagtagaaa ttaaagcaga aataacaatt tattgtattt
     8821 gtaaatagat aataaaaata gatttatttg gtgaggagat attattatgg caaagatatt
     8881 aggtatttgt ggaagtccac gtaaaggagc aacagaatat gcagtgttag aagctttgaa
     8941 agaagcagaa actatacctg gtatagaaac tgagtattgg tcagttagag ggaaaaaaat
     9001 aagtccttgt gtacattgtg atgcatgtat aagaaaagaa actatgtgta ttataaaaga
     9061 tgatatccaa gaattagagc aaaaaatatt ggaggcagat ggaattatag taggttctcc
     9121 agtttatgat atgaatataa ctgctcaact tacagcagta tttaatagac ttcgtccaat
     9181 gtatttagtt catccaggaa aacttcaaaa taaggttggt gcagctataa ctactggtgg
     9241 aactagatat ggtggccaag aattgacaaa acttcctata ataaatttct tcctaatgca
     9301 tgaaatgtta gtgtctggag gtttaggtgg atgctatata ggtggtacaa tatggagtaa
     9361 agatggaaaa gcaaaaggtg cccaagagga tgagacaggt atggacacag taaaaagact
     9421 tggaaaaggt gcagcagagg cagttatggt ttctaagttt ggtcttgaaa aatggaatgt
     9481 cgtaaaagaa gaattagatg ttaaaaaaga tgaaaaatca cctcttagag accactaaag
     9541 atttattata ttattgaata tatacaaata gtgatgtaat agattattgc atcactattt
     9601 tatgttttaa tttagatttt ataaatttta tattaaaaaa tatagaaaca aagtgttttg
     9661 agatttattt ttaaatttta aaaatgagaa ttaaaaagtg agaagataga aaaggttttt
     9721 tagttattaa attctacata cattgtatga actaaagata gaaaagaaaa aataataaaa
     9781 gcattgcaat ttctatacta aagagtcatg gaaaaatatc tagtatatat ttagtattgg
     9841 catgttaatt gcttatatat taaagtataa aaatttaaca ataatttgga ggtattttag
     9901 atgttaaaag aaattaaatg gaaagtaaat aatttgccaa agggcgataa ggaaaattgt
     9961 atcaaatttt taaatgaaga agaaataact aaggttagaa attttcacaa aagctttcct
    10021 caatataaag aaactccatt agcaaaccta gaaggtttag caaaaaaatt aggtgttgca
    10081 ggagtatatg taaaagatga atcttataga tttggattaa atgcatttaa agtattaggt
    10141 ggttcttatt ctatgggtag atacttagct caaagattag atacagatat atctgagtta
    10201 gggtatgaca aattaacttc aaaagaaata aaagaaaaat taggagaaat aactttcttt
    10261 acagctacag atggaaatca cggtagagga gttgcttgga cagctaataa attaggtcaa
    10321 aaatctgttg tattaatgcc aaaaggttct tctgaattta gattaaataa aattaaagga
    10381 gaaggtgcag atgctagtat aacagactta aactatgatg atgcagtaag attagctaat
    10441 gattatgcag aagctgatga ccatggagta atggttcaag atac
//
LOCUS       DAMJYC010000125        10175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJYC010000125 DAMJYC010000000
VERSION     DAMJYC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.131"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..874
                     /locus_tag="PC472_003482"
     CDS             <1..874
                     /locus_tag="PC472_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2728782.1"
                     /translation="KEKYKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIY
                     IENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDS
                     VGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENY
                     QSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKAT
                     EIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTI
                     RASFPKFSGGDFFE"
     gene            867..1514
                     /locus_tag="PC472_003483"
     CDS             867..1514
                     /locus_tag="PC472_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728783.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1643..2578
                     /locus_tag="PC472_003484"
     CDS             1643..2578
                     /locus_tag="PC472_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728784.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINDLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2592..3689
                     /locus_tag="PC472_003485"
     CDS             2592..3689
                     /locus_tag="PC472_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728785.1"
                     /translation="MKLYMSVKTMLKGLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3692..4756
                     /locus_tag="PC472_003486"
     CDS             3692..4756
                     /locus_tag="PC472_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728786.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5000..5497
                     /locus_tag="PC472_003487"
     CDS             5000..5497
                     /locus_tag="PC472_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2728787.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5673..5921)
                     /locus_tag="PC472_003488"
     CDS             complement(5673..5921)
                     /locus_tag="PC472_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728788.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5987..7003)
                     /locus_tag="PC472_003489"
     CDS             complement(5987..7003)
                     /locus_tag="PC472_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2728789.1"
                     /translation="MEYNGLLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7061..7828)
                     /locus_tag="PC472_003490"
     CDS             complement(7061..7828)
                     /locus_tag="PC472_003490"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2728790.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7896..10085)
                     /locus_tag="PC472_003491"
     CDS             complement(7896..10085)
                     /locus_tag="PC472_003491"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2728791.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3533 a         1570 c         1189 g         3883 t
ORIGIN      
        1 aaaggaaaaa tataagtaca tattgttctc ttttgttata atatcttctc ttattattag
       61 tttagataaa aatatatatt tggcactgga gtcaagctct atattgctaa ttataactgt
      121 actttctata tatatagaaa atgagaattt atcaaaatta tacagtcaaa atctttatga
      181 taagcttaga atttctaaag atgaattaaa aaaagttaat gctgatttgg aaacttatgc
      241 aagttctata gaagaattag caattttaaa agaaagaaat cgaatatcaa gagaaataca
      301 tgatagtgta ggacattctc tttctacaac aataatacaa ttaaatgcta tagaaaaact
      361 cttaaaagac aaacctctaa tttatgactt agtacatgaa cttagagaat ttgtaactga
      421 aagttttcaa gatgtaagaa gggctatatc tgagttaaaa cctgttgaat atgaaaatta
      481 ccaaagttta tttaaaatca aagaattagt taaaagcttt attaagctta caaatattaa
      541 tgttaaatta actatttcaa aaaatacttg gaacttatcc agaaatcaat ctatagcttt
      601 atatagactc atacaggaat ccctttccaa ctcatctagg catggaaagg caactgaaat
      661 cagaattttt attacattca atacttcaaa tcttataatt actattagtg ataatggtat
      721 aggatgtggc aatataaaaa aaggaaatgg acttaatagt atcaatgaga gaatatatga
      781 attaaatggt aaagttgaat ttgataattc taataatggt ttcacaatta gagcttcttt
      841 tcctaaattt tcagggggtg atttttttga ataaaattaa agttcttata gtagatgacg
      901 aaaaattaat tagaaaaggt cttaaaatta tattatcatc ttataatgat ttagaaattg
      961 taggagatgc ttctaatgga tatgaagctt tagaattttg taaaatgaat gatgtagaca
     1021 tagttcttat ggatatacga atgaaagtat gtgatggggt tctaggtaca aggcttataa
     1081 aggaatataa taattctata acattattaa ttttaacaac ttttaatgat gatgaatata
     1141 taaaagatgc tatgaagttt ggtgcttcag gatacctttt aaaagatagc tctgatgaag
     1201 tattgcatga agggattcgt tcttcatttt ttggcaatat tgtgcttgat aaaagtgtag
     1261 ctgaaaaaat catgacctct gaaaaaacgg ttaaacaaga gcatctttat gacatgtaca
     1321 atttaacaga aaaagaaata tctataatta gactaattgc aaatggactt aataataaag
     1381 aaataagtca agaactattc ttatctgaag gtacaataaa gaataacata actaatattt
     1441 tagctaaact agaacttcgt gacagaactc aactagcaat ctttgctttc aaaaataaaa
     1501 ttgtaataga ataaatctaa aaatataaag tgacttaagt cacttttttt agtgacttat
     1561 agtaatagaa ttataattca caattcttta ttataatatt atagcttttt aacttataca
     1621 aaattatttt ggagggatta ctatggatat agtcagagta aataatataa caaaaagatt
     1681 taatgataaa ttagtacttg ataacatcag cttttctgtt aaaaaaggtg agatatttgg
     1741 acttattggc ccaaatggtg caggtaaatc tacacttata aatataatta caaatcttat
     1801 gttaccaaat agtggtagta ttcaaatcaa tgatttagat ttatcaaaag attatataaa
     1861 agcaaagtct ataatagggt tagttccaca agaattagca attatagaaa ctcttactcc
     1921 ttttgataac ttagagtact ttggagcttt ctacggttta aagggaaaac tacttaaaga
     1981 aagaatcatt gaagcattag aagttacagg tcttactgaa gtcaaaaaga aaaaggtcaa
     2041 aaaattatct ggtggtatgc aaaggagatt aaatattgga atagcactct taaatcatcc
     2101 taaaatatta atattggatg aaccaactgt aggtgtagac ccccaatcaa gaaatcatat
     2161 tttcaatttt ataaaagata tatctaaaaa acatgaaact actgtaatat atacttccca
     2221 ttatatggaa gaagttgaac acctttgttc taaaattttt attatggatg aaggtaaaga
     2281 aattgctttt ggagataatg attatttaaa atcactcgta tctacaaata caaaattgat
     2341 tatggaaata aaaaatataa atgcccaact tatctttgat ttaaaaaata ctaaaggtgt
     2401 tatatctgtt ttagaaaata attcattatt agaattggat atagataaaa aattacaact
     2461 tacagatata ctctctataa ttgacaaaaa cgattcaaaa ataatgaaaa tttcttatga
     2521 agaacctagt ctagaagatg tatttttaaa tctaacaggc aaaaatttaa gggactaatg
     2581 gaggtgattt tatgaaatta tacatgagtg taaaaactat gctaaagggt ttaaaaagct
     2641 cctttatact taatttaata tattttttag ctttacctct gattttatct tggtttcttg
     2701 gtatggttac agaaagcatg tttcaaaacc ctataaaaac agaatctacc cccattgtta
     2761 tatatgacaa agataacaca agattatcaa atgatttgac aaagtatttg aaaaatgatt
     2821 tatcttatat attaacagtt aaaaaagatg attctaaggc agaattaaag cttactatac
     2881 ctaaaggcta tgagagtagt ttattaaatg aaaaatcaaa tactctaaac atagaaaaac
     2941 ttggaactcg tgatgatatt gcaatattgc ttcaagatat attagatact taccatgaaa
     3001 aattttattt aaataattct cagaaaataa gttcagaaga tttttctaaa ttatttaata
     3061 aaaattctat tgatacttct ataataaata acaatgttaa acagagctct tatgaatact
     3121 ttgctttagt ttcactagga tttcttgtaa taatttttat aatgaacaat attttatcaa
     3181 attatataag tgaatcaaag ggactttcaa aaagacttta ttctatgcct ataactagag
     3241 tacaattctt aatttatgac tttgtaggtt tatggattta ttcatttata ttcttattat
     3301 tatatgtttt attctttaga atacttggaa taacatttaa aggtaatttt gccatattgc
     3361 tcctactttg tgctttatca tcttatttta tgacttcaat atctaccttt gtaaccagct
     3421 tttttagcaa aaaatacggt acagcaatag tttatgcatt actcttttta cagacaattt
     3481 ttggaggtat cttcagtatg ataagtgata catttactaa gcttacaagt ttatctccta
     3541 cctatttaat aggagaattg tttagtaatt atgaaacatt taaaactata gattcaatag
     3601 gagatttaat tttcacatgt ttaattatct ctacaatatt aattgcactt gctattgtaa
     3661 aggaaaaata taaatggagg gaggtttaat aatgattaga ttaactattg caaatataaa
     3721 aagatatcta aaaaatcata ctcttttaat taacatggtt atgcttccta ttatactaat
     3781 tttttcactt aattttttta tcaacaactc aggaaatcaa tcaatgtact tttctccagt
     3841 agcaattgta tctgactctt caggaaagta tgaacataag ttaataaata gttctaaatt
     3901 aaaagaaaat tcatttagat taaatgaaca agataaggct atgaatctat taaaaaataa
     3961 taaggtttct gctgtattcg ttttagataa aaatttttca aatagtatag ataaattaca
     4021 acgacctgta gttaaatgct ttaaaattga aaatggtggt ggtagtcttt gggcagaatc
     4081 acaaatagaa tcttttatta ctaaatcgtt aaaattgaaa gtggataaaa atatagatga
     4141 taaattgaca aagacaaata taattgataa caaaccagat aagaataaag gttcattttt
     4201 agtagtcttc ttgatatgct attttatgta tattaatgcg gcacatcttg cttctgattt
     4261 atttacatta aaaaaatcaa atgtgctaaa aagacttatc tctacagata ataaagatat
     4321 aaaaataatt ttcagtatat ttttaggact attttttatt caatcaattg tatacacttt
     4381 tgctctatta tgtttttcaa ttatagaaga ctttaatttg agtctgaatg tattgatgat
     4441 tgtactagca aatagttttg tttccactgg atttgtattt tgggtagcta gagtatttaa
     4501 aaatgaaagt tctataagtc ttatatctac attttattct ttaataggtc tagctatatc
     4561 tatctctagt ctaataccat caatggataa attatccttt atgactaatc tttcaaagtt
     4621 cacaccattt tattggacta tagatgctat taaaaacaat ggtagtattt taataaatat
     4681 aatagctcta atattaattg gaattatatt tgtaactgca ggaagtctta aactaagaga
     4741 ttttgctaaa aactaataat tattaaatat aattagtatc tatttttata taattgtaaa
     4801 agaatatttc aacatattaa attattttat cttatatttt tctctttagt ttcacacatc
     4861 accaagtcta aactttatat tatatattaa tatttttgac ttgtcaagtt atttatgtta
     4921 taatattaat cattagataa ttaaagcatt gtacctaagt tgcaatatct aaaattcaat
     4981 ttggaggtat aactagcata tgtataaaat tggtgaaagt gtaatgtatc caaaagaggg
     5041 agcctgctct gtaaatgata ttgtaacaaa gaaaataaat catgagatgc aaaaatacta
     5101 tgaattaagt gtcattttta atagtaacct taaaatttcg attccagttc taaatgcaga
     5161 tagaattggc atacgtccag ttatggatgg aaatgatgtt gataatttta tccaatctat
     5221 taataaaaca gatggtgtat ggatttttga tagaaaagaa agacttaaac tgtaccaaga
     5281 taaatttcat tcaggagatg tttttgaaat tgtaaaatta atcaaaatgc ttatgataca
     5341 agacagttca aaacaattgt gtagcacaga taaggagttt ttaaataaag ctcaaaaatt
     5401 tgctttatca gaattagctg ctgctcaatg taaatcctat acaatggtat tagaggaaat
     5461 gaaaaaacat atattaaatt caaagaatac aaattaaacc agttttttaa actggtttta
     5521 ttttttatac taatcatcca tgctacttta gcttatttta aactgcctaa tctaatatta
     5581 ataaagttat ctcaattaga tagatgtatt ttattcaaat aaaaaaaact aagagtaaat
     5641 catattactg atttactctt agctaataaa atttaatctt cttcaagttc ttttttctct
     5701 tgtaataata cacttaattc atttgcttct aaaacttgtg cctctgcaat atcaagtaac
     5761 tcttgttgcc attccacaca tttatctttg taatcaaaag gtagcgttct gtcaactatg
     5821 tacttatcaa tacagtcctc cgcactttta attatttctt ttctttcatc aataaatttt
     5881 tctaattcta ctaaggcttc ttcataagct tttctactca tataaattcc ccctttgtac
     5941 aattattcta cattaaaatg atttttcctc ttttttgtat aaaattttat ttgtatataa
     6001 cttttccatc aatatctttt atagtttgac atccagttaa tatcatagaa ctacttagct
     6061 ctgattgaag tttatttaca taagtttcta caccatcagt tgcaccacca aaagaagcag
     6121 tcacaaaagg tcttcctatt aatactgcat ctgcacctaa acctatcatt tttagtacgt
     6181 ctaaaccagt tctcactcca ccatctgcaa gtattgtaac tttaccttta acagcatcag
     6241 caatttttgg taaaacttca catgctcctg gtgtacagtc aagcactctt cctccatgat
     6301 ttgatacaac tattgcatcc acacctgctt ctactgccat taaagcatct tcaacagtca
     6361 ttataccttt caatataaat ggtagtttag tagactttac taactccttt atttgtttaa
     6421 catttttagg aagtactggt tttccatgta atgataaagt tactagacca caggcatcta
     6481 tatcaactcc aactgcaaat gcaccagctt cttcagctaa tcttatcttt tttataatat
     6541 tttcattatc ccaaggttta ataaatacaa ttccagctcc attatacttt ttgactacat
     6601 ctaaattttc cattaaaaaa gcatctactg ccgtatcacc aaccattgca taaataccac
     6661 tattagcaca tcctgcaact actggttcta tgtaatctct ttcattaatt tttccaccca
     6721 tatttaaagt agttcctgtt actggagctg caaatatagg tgcactcatt ttctttccaa
     6781 acatctctat agatgtatca ggatttgata catcatgtat tactctcata tttactttta
     6841 ctttttctaa actcttgcta ttttctataa aagaagaacc acttccttta cctcccatgc
     6901 caggcacttc acctgcacaa gcaaccccat tacaaacctt acaaacttta caacttccat
     6961 ttaaattttc tctagcactt tttaataatc cattatattc catctttaat cccccattaa
     7021 ctatagtaat ttatattata tacattatat catattatat ttacagtata tttttcaaaa
     7081 aactttttct gtggatagga gttatgccat atttttcaat agcttcatag tgttctttag
     7141 ttccatatcc tttgtgagat ttaaacccat attctgggta tactctatca tattggtaca
     7201 ttatactatc cctagtaact ttagcaagta tacttgctgc tgctattgat atagattttg
     7261 aatctccttt cacaattgga ttttgagata tgtcaatacc tggtattgtt gctgcatcaa
     7321 ctaataaata atctggtgct ttttttaaac aatttatagc ctttttcatt gccatatatg
     7381 tagcatttaa tatattaaat tcatctattt cttcattgtt tactatacct attccataat
     7441 ctagtgcttc ttcttttatt acttcaaaaa gttcatctct ttttgcctca ctcagtttct
     7501 tagagtcatt tacaccttct attttggtat cttttttaaa tactacaaca gaagctacaa
     7561 caggtccagc aagtggccct cttcccgcct catcaattcc tccaatatat aaataacctt
     7621 tatcatagcc ttcgttctca aatatattta tagtttccaa tctttcttct tcttttctaa
     7681 tattatccag cttttttgct agttttatag ctagaccttg tacagatttt ctttcatcaa
     7741 ctcttaacag ctctatatat tccatatatt tttctacttc taaagtctct attatttctt
     7801 taatctccct cacactttta tcctgcatac cattccctca tctttcgttt ttaaaaattt
     7861 aatttttaaa tatattaaaa ttttttaatt agagtttaat tgcatccact atagccacat
     7921 tttaaacata tactacatcc acctgtatgt gcaattcctt cacttccaca ctcagggcac
     7981 acaaattttt catcttcttc aaaattttta tttattattt ctttggcttt ttcatcttta
     8041 ttatcttttt cttttttatt atttttataa tattcttttt tactattttc ttttttatct
     8101 tctttattat tttcttcctt tttttcattt attaaaattc cactcctttt acatccatct
     8161 ctaaatattg ttactccttt aagaccagcc tcccatgcct taatatatat attgaaaaca
     8221 tcttcaactg tagtttcaaa aggtatattt attgtagatg atatagaagc atcaatatac
     8281 ttttgccata ctttttgcat gtttattctt tcttcatatc ctatattcat agcagtaaca
     8341 aaaaaatctg gtaaacattc ttcatctgta atacctttct tgtccatata ctctttaaca
     8401 attggagtat atactttata atacacatcc tcatcatgca agctttcagt ttttcttata
     8461 tatgaaagat taaatatagg ttctattcca ccactgatac ctatcattgt agatatggaa
     8521 cctgtaggag gtatggttaa aagttgtgaa tttctaagcc catacttttt aaccatttcc
     8581 ttagtgtctt catttagatt ttctataaaa aacttagatt tagaaatatt ttcatattta
     8641 tactcactaa atgttccata ttctttagca attaatgctg attgcttcac agcttcattt
     8701 aacatagtct taccaattaa atcacatagt tctaaagatt catcagaacc atatcttatt
     8761 cccattttta taagcatatc agctataccc attacaccta aacctatctg tctatactta
     8821 tgtacactat ctctttgttc ttgtaatgga tgcaagttca atccctcatc taagacatca
     8881 tttaaagcta ctacagcttc tctaacgcaa gattttagtt tattaatatc aaaagtagca
     8941 tgttcaccaa atggttgtat tataaactct gacaagttta ttgagccaag tagacaactt
     9001 cctccacttg gaagtggttc ttctgcacaa ggatttactc ctgaaaagca aaagttctta
     9061 tcttcactca taaaattata tgtttttatt ttatcccaaa acaatatacc tggttctgca
     9121 taatcccagt tatttttcac aaatttatta aataaatcat aagcatttac tatcttaact
     9181 attttttctc cagtagtttc aacttcaaaa gtacaattat ataacttttt ttctcttaca
     9241 gcttgcataa attcatctgt tactctcaca gaaatatttg ctttagtaac cttctccaaa
     9301 tctgttttta tatctataaa gtcttctatc tctggatgat ttacatccat agaaatcata
     9361 agagctcctc ttctccctct ttgaccaatt aagccagttg tcatactgta aagttccata
     9421 aaagagactg ctccagtcgt atgttttgca gcattgttaa ctttcgctcc ttttggtctt
     9481 agctttgaaa tatcaattcc aactcctcca ccataactaa aagttctagc aagttttttt
     9541 gcagtatcaa atatatcttc tatgctatct tttggtggtt ctattacata gcaattagaa
     9601 tatgttactt tttttccctc tttctgtaat cctctatttg ctaaaattct tccagcaaat
     9661 agaaatcttt tttgtcttat tagttttgat attcttgaac tattatttga aattctcttt
     9721 atccattcat caaaactctc accattatgt ctatacttgt tcttccatat tgatttttgt
     9781 aattcatcaa actcccaagc tttagtcctt atttcagagc gtctttctct atataatatg
     9841 tagtgttttg ctatatcttt tctatttgat tccataagta gtacttcaac tatatcttgt
     9901 atttcttcta cggataagca tttttccata gaattatagt cattagctat ttttcttgat
     9961 atatcatatg ccaactctct atctacgcca ccttcactat ttatactagc tttatagaca
    10021 gcatcttcaa tcttactttt actaaaaagt tcttttgtac catctctttt tattatgtac
    10081 ttcattttta aacctccaat aaattagtat taaattaata cacacattaa ctataactct
    10141 aaacaaactt tttatatatt tgtatatttt catat
//
LOCUS       DAMJYC010000126        10109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJYC010000126 DAMJYC010000000
VERSION     DAMJYC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.132"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(150..2030)
                     /locus_tag="PC472_003492"
     CDS             complement(150..2030)
                     /locus_tag="PC472_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2728792.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGVDRFETA
                     VKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRISGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2241..8183)
                     /locus_tag="PC472_003493"
     CDS             complement(2241..8183)
                     /locus_tag="PC472_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2728793.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKSEFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDKICEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENIEQIDNNSNNKDENSDKQE
                     ANSNTNQDKNNDKENSSMDKPDLGEDTPKKESNISKDDNSTTSTYSRSVLETYADVQN
                     SDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNLYKEIPKPEMNIPKINK
                     ISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTIEVRDIEA
                     YGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEFQVVPDTK
                     PEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIRGTSSKIE
                     GLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKENGGFGIPT
                     NHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRGPIITFDK
                     SYTMDTFMVTPRFDVSDKTINDFEVRYWDENGQLHNLGNVPFETKTDPVNNNKYVMVR
                     LKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVELKEDVTQE
                     KITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQEINKSGK
                     YKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYTQYYGESG
                     AYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVAGATKIPH
                     LNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSYGYSEKTG
                     VLNTTDIESDKVTLNVPATAIYQGISKGNADLNTQVDRFYNSMLAWEQIMDLVYSERG
                     VFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHVGVEYGSV
                     PGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLALLAQTFD
                     DKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGSMLKNNSD
                     VDLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFKAWGVEIT
                     PELNSIMDSKNYEKESRKIQYVNDEARRKTLNENIASMAKDTKVSASFSDGIQSGSLV
                     KSSKISIDLDVDKDKDKILGYEIIRSDGNYLEGENGTQVKYRTVGFVDAKGNSKAKFT
                     DNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITNTVSDGDV
                     RTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEENPYIIVD
                     MKETLPIVGLKYTKPKTETRKFSLKGLFNFRKDTSTTYNPLTNYKIQVSNDKKNWEDV
                     SQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIEVAELDII
                     GPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETAKIPAGSIVITGEY
                     SGNPAFNLPLLIDKNNKTISGESLLFAEVPEKGELGEISKGTWVYYMPKENFDSLSDK
                     VKAELYRYNDLQGDTPVGQRFVSDTMCVPVGAKNYDELKTISLTDSNSKARKATRNAI
                     DVSNKKVITVSNRVKVNIVRDK"
     regulatory      complement(8307..8392)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8564..9967)
                     /locus_tag="PC472_003494"
     CDS             complement(8564..9967)
                     /locus_tag="PC472_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2728794.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2789 a         1873 c         1146 g         4301 t
ORIGIN      
        1 atttaaaatt aaacttaata tagctcaaat tattatacat tttaacctca atattattaa
       61 attgttaaga taatgaaaat aaaataagaa tgcatttaaa tatataactt tatatctaaa
      121 tacattctta ttttctattt acttaatatt tatttttcaa ttaaactctt aatcttatct
      181 attacagatg aggctattcc tccacctact tcaaacaact ttgatgaatt tttacctttt
      241 aatgacgatt cttgtgattt atctaacgta tttgtagcaa gtataactgg tgaattgttt
      301 ttagctgcta atggtccaac tgctaaagcg tctataagtt tgtcttgatt tgcaactaca
      361 actccatcta gcttagattg agagtagaat ttatctatta ctttggcatt cgtctcttgt
      421 ctagtttgac ctgccaccct attgtttgat atatctttgt ttgctatttt tccaacttgt
      481 tcaatagcct tgttagatat tttttctact ccaccaataa aatatatatt tgacacctta
      541 tttgatttaa taaattgttc agcttcatta tctacaccat ctttttgtgt aagtactatt
      601 ggagtttttt ctttaccagc cagtgatgct attgataatg aatcagcttc tccatttcct
      661 gctcctatat acaacttgct tacattgttc gtcttatcta tttcttttgc tatattaata
      721 gaggtttgat atcttgtatc tccacttatt ctttgtactg atatttttga atttacagct
      781 ttgatagctt caacaactga atttggcatt gagttgtttc cacctatcac aattactttt
      841 gatggattta gccttttcaa ttcttctaca gttttttgag gaagcttttc atttgatgat
      901 aaaagaatcg gtgagttttt tacactagca agcggtgtag ctgtaagccc atccaccata
      961 cttttatctt ctccatttac tattacaact gtatctgatt ttgtccaacc actttgagat
     1021 attttgacag ctgtctcaaa tctatcaaca ccttttaatt ttttgactgt gacattagat
     1081 gtagaatcac ttgaatttcc tcctccagag cttccaccac tagacgaacc gcctgaatta
     1141 cttcctccac cattatttcc accattgcca ttatctggtg gtaattcatc tggtttggat
     1201 aattcatttt caactatgta ttctaaactt tttggaagtg taacttcaac atatttattt
     1261 attgtagctt ttcttccctt agttgttttt tgtgctaatc caagttttaa tattacattt
     1321 gaaccacttt cttgtgtttc tttactaaca atagaatagt ttatcctaat attgtttaat
     1381 aaatattcaa gtgcttcact tttctctacc ttatactttt cagggtcatt tttactagaa
     1441 ccagttccac caattggttt tacttcactc tcattaataa gggtaaatat taatccttca
     1501 tcagagttta tattttcata atccactgtt ttgttgttat atgttacaat ttttaaagct
     1561 ggtttatcgc ttgttccttt tcctaaaact ttatagtcag catttgagtt actaccagat
     1621 ttttttacag atattcttcc tatattcaat ctatttccgt ctaataaatc ttcatttgat
     1681 gtaacatata tatctaatgt agttgtatta cttgatttgt tgtattctgc tctacaagta
     1741 gcatttgcat ttccactttt tatctgagat tttatattat cagaccaagt aatcttttct
     1801 ttctgctcat tatctactac ttcaacctca ccttgtactt ttatactggc ttgaaatgaa
     1861 tatactatgt tttctactga gtccacagat atatctatag tatttacttt ctcatacgtt
     1921 tttgttcctt gtaaaactgg agctgcatat gcacatgtag cactaccagt tacagctact
     1981 gccatagttg atactgctat agttcttata aaagattttc ttttactcat taacacccgt
     2041 ccttatctat cattaactaa gtggataaat ttacttatcc acctagcttt tatatttaat
     2101 ttataaatta ccctaaactt atagttacat ttatatactt tacttttaat atctactatt
     2161 ttaaaactat tctaatacct aatcatatta gatatacaat gttagtatta gtatttttac
     2221 gttttttaga gttccaccta ctatttatct ctaacaatat taacctttac tctattgctt
     2281 acagttataa ccttcttatt actaacatca atagcatttc ttgtagcttt tctagcttta
     2341 ctattactat cagtaagact tattgtcttt aactcatcat aattcttagc tcctacaggt
     2401 acacacattg tatcacttac aaatctttga cctactggag tatctccttg aaggtcattg
     2461 tatctatata gttcagcctt aaccttatca cttaaagaat caaagttttc ttttggcata
     2521 tagtaaaccc atgtaccttt actgatttca cctaattcac ctttttctgg aacttctgca
     2581 aataaaagcg attctccaga tatagtctta ttgtttttat ctataagtag tggtaaatta
     2641 aatgctggat ttccactgta ttcacctgta ataactattg aacctgctgg aatcttagct
     2701 gtttcatctt cacttgtgct agggtcatca ctttgatata cataatcttc tgctaacttt
     2761 ccaattccat tagtacgagt ctggtctgca ttattcattg tgccaatttc tatattatca
     2821 cctggaggtc ctattatatc taattctgca acttctatat tagacttatt atttgagatt
     2881 agttttacat atctagcttg atatgtccaa agtttaccat ctttattaaa tgtaactcta
     2941 gcttcattat cttggtcttt tcctccacca actaaatctt gtccatattc aaaagttcct
     3001 tgacttacat cttcccagtt ctttttatca ttacttactt gtatcttgta attagttaaa
     3061 ggattgtatg ttgtgctagt atcctttcta aagttaaata atccttttaa tgagaacttt
     3121 ctagtttctg tttttggttt tgtatatttt agtcctacta taggtaatgt ttctttcatg
     3181 tctactatta tatatggatt ttcttctaca tttatatcag ggtctttttg agggtctttg
     3241 ttccactctg ctttacttat tatctttcct ttatatgtag tacttgcatc attatctttt
     3301 atattatcaa tttcaggatt ttgtaatggc ccatgtgagt cattttcagt tctaacatca
     3361 ccatcagaaa cagtatttgt tatgaatgac catgctgatt tatttatttt cccttcatct
     3421 gataatttta cagtaccaac ttcaaattct tctgttggat ttaggtgata atcatatgca
     3481 actaccttat atgtaaatgc tctgttatta agaggagata tattatcagt aaatttagcc
     3541 ttactatttc cttttgcatc cacaaatcca actgttctgt attttacctg tgttccattt
     3601 tcaccctcta aataatttcc atcacttctt attatttcgt atcctaatat cttatcttta
     3661 tccttatcta catctaggtc tatagatatt ttactagatt taactagaga accactttgt
     3721 attccatcag aaaaactagc acttacttta gtatcttttg ccatactagc tatattttca
     3781 tttaaagtct ttcttctagc ttcgtcattt acatattgta tctttcttga ttctttttcg
     3841 tagttcttac tatccattat actgtttaat tctggtgtaa tttctactcc ccatgcctta
     3901 aagtaatctc ttaaatcttt ttcaacaaca cttgatgcca ttgctactag catttggtct
     3961 tttggtaaac tattaattgg gtcactgctt gaaagtgctc tgtattttct tgaaatcttt
     4021 gcatagaatg tgtcattact taaatcaaca tctgagttat tctttaacat tgaacccgta
     4081 aaatcatcat cataagctag atgtaattgc cataacattc caagcctttc aaatacattg
     4141 cttggtatac ctaagctatt tgaagttaca tgttcatata tcttatccat tgttgaacct
     4201 tcaagtcttg aatgtgtctt atcatcaaat gtctgggcaa gtaaagcaag tacattgtta
     4261 ctagtttcgc tatatgtcat ctttccaata tctgtaacat gtcctatttc atgacctatt
     4321 ccccatccaa ataaatttcc tcctgtagct tttccactat catctatttg gaaaggtact
     4381 ccattcataa gtcctggaac tgaaccgtat tctactccta catgtaatcc agaagcatac
     4441 ataaatgctc caataaacat tctttggtat tttatgttca tacgagattt tggagccata
     4501 tcatttttag ttaagttaaa ttcattatca tctacagttc cattaccatc taaatcttgt
     4561 gttttaaata ctcctctttc tgagtaaact aaatccatta tttgttccca tgcaagcata
     4621 ctattataga atctatctac ttgtgtattt aaatcagcat ttccttttga tattccttga
     4681 tatatagctg ttgcaggaac atttaatgtt actttatcac tttcaatatc tgtagtgttt
     4741 aacacaccag ttttttctga atatccatat gaattaccaa catttgttcc tggataatat
     4801 ggttctcctt ttacagcatc tttatattta gctaattctt ctatatatgc ttttatcttt
     4861 ttcttagatt cagaaactcc cgcaggactc ttatcattta ttgttccatt tagattaaga
     4921 tgtggtatct tagtagctcc tgctacacgt acttttatat ctcctccact tggagtatct
     4981 tcagggtatc ttatgtataa acttcctcca cattctatat ctaaactatg aagtttgctt
     5041 aattgaatta cattttttcc agttttaaga tttatttctc cactcttata tgcaccagat
     5101 tctccatagt actgtgtata tatcaactta ggagtttttc cacttttact tccaacatat
     5161 actgtgattt catctcctgc tctagcagct actcctaatg cctgccagtc attttgcata
     5221 cctaaattat tggaatttac agtatactgt ccgtttttat ctttatattt tcctgattta
     5281 tttatttctt gatttacagt tgttacatca gtaagtgtgg ctctatcatt atataagtct
     5341 tgagctaatt ttaattctct atcaataact gtcttatatg gatgatattc tccactagct
     5401 ttatctggag tatttaatct atcttttaat tcagttattt tttcttgagt tacatcttct
     5461 tttaattcaa cttgtaggtc atccataaat agatttctta catcatcttc tatactgtca
     5521 tactcataaa acttaagttc tgatatatta gacttatatg aataggatga acaaacactt
     5581 acttgtatct tggtagcagt tattggttct tttaatctaa ccattacata cttattatta
     5641 tttacagggt ctgtctttgt ttcaaaagga acattcccca gattatgtaa ttgaccattt
     5701 tcatcccaat atcgtacctc aaagtcattt attgtcttgt cagaaacatc aaaacgaggt
     5761 gtaaccataa aagtatccat agtataagat ttatcaaaag ttattattgg tcctctattc
     5821 gttccctcag ctgcattata tgttgggaat gtccaatgtg ttgtaaagtc cccatctact
     5881 acattgaatt tatcaaatgg atttttatca ctatattcac ttttgtcata tcctgctgga
     5941 taagtaacat ctacaatgtg atttgttggt attccaaatc caccattctc tttaggagta
     6001 ttaattaatc tatagttagg tgttgttggt gcatccatgc tttcagttgt acctatatat
     6061 gttttagaca ttccactagt tcccaagtgg ttagtagctg tcattctaac ttcatatgtt
     6121 gtattatctt taagaccttc tatctttgaa cttgtcccac gaataagctt atcattgtat
     6181 tttatccaag acccattaga gcctttttct ttatagtaca agtcaaagtc tttagcttgt
     6241 ttatgatttt tccagcttac atcaagagat ttaaatttac ctttaacatt tataccttct
     6301 ggtggttctg gttttgtgtc tggaacaact tgaaattcta atatactatc tgggctaaat
     6361 tgtataggtt tatagtttga atctacattt tctggtactc catctaagtt ttctggtgtt
     6421 gcatctttat aaccactaga ccaatctcca tttaaagatt gtatgctaac tctatatagt
     6481 ccatatgctt ctatatctct aacttctatt gtatttttac tagtttgtaa ttttttagta
     6541 gataatactt ttcccttact atcaatttta tctagtataa gttcgtatgc tgatacattt
     6601 acttcattat tccaataaaa agatactttt tcatttccaa cagcagtgct agttgatatt
     6661 ttatttattt taggtatatt catctctggc tttggtattt ctttatataa attgtttaaa
     6721 aattcaactt ttgatatttc tgcaagattt ctatcgtttg ctgttgtagc tgttattttt
     6781 atagttatct tttttactgc tacttgttta ccaagattta ttactatatc gctattttga
     6841 acatctgcat atgtttctaa tactgaccta ctatatgtag atgttgtaga gttatcatct
     6901 ttactgatat tactctcttt ttttggagta tcttcaccca aatcaggttt atccattgat
     6961 gaattttctt tatcattatt tttatcttga ttggtattag agtttgcttc ttgtttatcg
     7021 ctattttcat ctttattatt tgagttatta tcaatctgtt ctatattttc atctttatta
     7081 tttacttctt cttgatttac ttcttcttgt tcgttattta cttcctcttg ttgctcctca
     7141 tcttttaagt ttttcatata ttctttgttt tcataaggaa tctgttgaac attaccatct
     7201 ttgtcagtaa cttcaactat tccttttgct ggagcagtac cttctggagc ttttatattg
     7261 atgacttcca tattaagtgc ttctgataaa gctatatcca ttactgctgg tttttcctca
     7321 catattttat cattttttga agatattgta actccattat tttctccact aaataaattt
     7381 tctacattac cattaatatc tacatcacta tttcctttta attcaacctt acttgtatct
     7441 actataggat tagtagatac tatactttct aggctttttg catcttcact ttgtcctaaa
     7501 tttttctgta cataatctaa gtcaacaata tctacctttc catctctatt taaatcaaat
     7561 tcacttttat ttgaatctat attctctaaa accttattat aatcactctt atctacttta
     7621 ccgtctccat ttacatcacc cattaagaat aattcataat ttaaaggttt atttttatta
     7681 tctacacttg ctctattgct taataagact ctttgtgaat aacttgatat atcaatgtct
     7741 ttaatttctt tattcttaaa tccagctcca gagacttcta cattgtattt tccaacaggt
     7801 aaattattaa atacaatatt ataatatgat atattctcat ctttattttt tatttcactt
     7861 ctacttgcag ataatttttt tatagtatat tctatattat ctgtcccaac atttactgtt
     7921 cctgaatctt tttctccaag ctttattatt ccactattgt tactatcatc ttttaatcgt
     7981 aactgcatat tagtcatact ttctgactcg ctatttttta ttggcagtga aaaatttaaa
     8041 tctacttcaa gactccccct taacttttct gtaactgatt tttcaactgc ccttagcttc
     8101 ccctctaata taggttctgc aaaaacgttt agtggtgcac tacacataga tactgctagt
     8161 acacaagata ttgctttttt catgaaataa caatcctccc ttaataaata tctaattttt
     8221 catgaattta aatactataa gtttcagaca ataaaaaaag acactcaaag agaatcttaa
     8281 ttataaaata aaaagacttt actcttttta gcaactggct gatatggctt taaaagcttt
     8341 aatccctata gttttgcgtc attagatttc cctaattttg ccatttaatt ttcactactt
     8401 tcttacccat tttatactat atttaaactt tttaatacaa tttttttaga ttaaattata
     8461 aaaaaataac atttatatta atttattaat ataaatgtta ttttccatta taatttaatt
     8521 atttatatag cttccttaca agcataattt tgtgtatatt ttcttattct ttatatcttc
     8581 cataaaattc atgtgtagca tatagattct tatctcctgt tatagctata ccaaatccca
     8641 ttatttcata ccctttatgc aacatgttag ttccttcttc tggatttgtc ttccactcat
     8701 taaaaataaa gtttcctata tcatctgcat ctctatttgt atatacaggt ttatctggaa
     8761 ttttatattt aattatattt tcaccttgaa ctgctatata tccaggttta atctcactcc
     8821 aatctaagta tttcaaaaaa gtagaatatg aattttttcc atttatcgta tgagataaag
     8881 tcttcttatt tgccattaaa ttagaccaat catttgctag accctctaat ctgcttgcta
     8941 cagataacgc tcttactcca ttctcttttc tatactgctt tgtaagagca tatactttac
     9001 cctttattct ctctttaaat gctctatcat ttggattgta atataaacct tgttgatttg
     9061 gtgaagcttg atttatacat tgcttgataa cattctgatt tatgcctcct agtacagtca
     9121 atttgtctgc accacttaaa atactcttat tactgccatt ttcaactaaa actacaggag
     9181 cattttttgc tattgtagag gcagccaaag cgtctgttaa ttgtaagcct ttacttagat
     9241 aaaattcttt actattttta taaaactctt gtataactat cttattagtt tcaaatctat
     9301 ctgtgccacc caatctaata gaatttgtgc tcttaactaa ttcgtcgctt attatttcac
     9361 tagaaccaag cgcataactc tttaagcctg tcgtgtcaaa tcccacacta tttccatctg
     9421 taagtattat aggtactcca tctctagcag ctactggtga tgcacttatc gcatctgctt
     9481 ctcctgtaaa tccattggct ataattactt tatctattgg cttaatttct ttgatttttt
     9541 cagctatttt tatacttgta tcaaatctat tttcaccaaa gattctttgt acaaccttct
     9601 ttttggatag taactgattc tcaacatttt tacttattgt tgattctgag cctattatat
     9661 acacagtctt tgctttgttt aatctttcca tagttgtgtt tggaatctga ttttgttttg
     9721 tcatcaaaat aggcgcattt aaggctccag ctaaaccact tgcacttaga ccatctgcta
     9781 agctattatc tgagttgatt agtattgctg tattgtagtt ttgcttatct gctatttttg
     9841 ctgctgtctc atatctgtca ttccctttta tttgctcaac tgatgtcaac gcatttacca
     9901 taggagtatt taccattact aaaaatagtg taagtcctat agctaatatc tttttactat
     9961 tcttcatatt attttctacc cccaatgtac ttttcatatt ttttattagt actcatactt
    10021 atttttaatc aaaagttaca attttgttac catattttat atatgatacc atagttatcc
    10081 atacaactca aacaatatac ctaaagttt
//
LOCUS       DAMJYC010000127         9917 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJYC010000127 DAMJYC010000000
VERSION     DAMJYC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.133"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            273..1211
                     /gene="pdaA"
                     /locus_tag="PC472_003495"
     CDS             273..1211
                     /gene="pdaA"
                     /locus_tag="PC472_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2728795.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
     gene            complement(1583..2956)
                     /locus_tag="PC472_003496"
     CDS             complement(1583..2956)
                     /locus_tag="PC472_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDF2728796.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(3143..4465)
                     /locus_tag="PC472_003497"
     CDS             complement(3143..4465)
                     /locus_tag="PC472_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728797.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4458..5162)
                     /locus_tag="PC472_003498"
     CDS             complement(4458..5162)
                     /locus_tag="PC472_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728798.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5345..6409)
                     /locus_tag="PC472_003499"
     CDS             complement(5345..6409)
                     /locus_tag="PC472_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2728799.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(6412..7110)
                     /locus_tag="PC472_003500"
     CDS             complement(6412..7110)
                     /locus_tag="PC472_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2728800.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYNYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(7101..8198)
                     /locus_tag="PC472_003501"
     CDS             complement(7101..8198)
                     /locus_tag="PC472_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2728801.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(8521..9765)
                     /locus_tag="PC472_003502"
     CDS             complement(8521..9765)
                     /locus_tag="PC472_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2728802.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
BASE COUNT         3451 a         1684 c         1099 g         3683 t
ORIGIN      
        1 attcaattaa cttctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacatctt ctcataatat ggtcaactat ttttttcatt ttttttaaat ttattgatta
      121 ttcacccttt gtcatactat tgtgacttta tttttaaaga tataactatt attaatagta
      181 atttccaatt ataataatac tatatgcaat tgtaattaat catataatta ttattatatg
      241 caattattat taatttagaa aggagcttca aaatgtttag aaaagtattg cattatttaa
      301 cactactttc tatttctatt ttttttattg ttggatgttc aaattcacaa aacaatcaaa
      361 acgaaaatca aaataaggaa acacagttac aagaagataa ggaaaaaata gatagtggta
      421 aagatacttc caatgtaata gtttcagacg gaacagataa accatcaaaa gccactacta
      481 ataatgataa taataaatta gatgtatctt ctttagataa cactactctt gattggtttt
      541 atataccaaa caataagcat aaaacaccag aagtaaatac ggacattgaa tttaaatttt
      601 ctgactatga tgccctatac aatggaccta caaaagatgg tcaaaaaaca ctatatctta
      661 catttgatga aggttatgaa aatggataca ctacaaaaat actcgacact ctaaaacaaa
      721 accaggttaa agcagttttt tttgttacag ctccatatat aaaagaaaat aaagatttag
      781 taaaaagaat ggtttctgag ggacatatag ttggaaatca tagtaaaacc catccatcta
      841 tgccaacaaa aacaagcaat ctaaaaaact ttaatgacga attatatgat gttgaaaagt
      901 tatataaaga tgtaactggg aaagatatgg ttaaattttt tagacctcct atgggaaaat
      961 actcagaaaa aagcttggca atgactaaaa atttaggata caagactgta ttttggagtt
     1021 ttgcatacag agactgggat actgataaac aaccatcaca tgaagaagca acgcaaaaaa
     1081 taatggataa tctacatgat ggttctatct tgttactaca tgcagtttcc aaaactagta
     1141 cagaaatttt aaatgatttt atatcaaatg caagaaaact aggatatgaa tttgaactct
     1201 tggagtatta atatttataa ttacatctaa agtctacaaa ctaatatagt taatataata
     1261 taggttataa aataataaat taaaattaga atttttgttt ggtgatagta taggtaaaaa
     1321 cttgtgatag cttatatatg ttggtgggac agggcaataa aacatcaaaa aatttggcaa
     1381 gatattaaat atagataaca gtattttaga tgttatattt agatgttatg tataatataa
     1441 tgaaaaatat tactttatta aagaatacta ataaaaatat caataaaaat agatgtatgg
     1501 cttagaatta gtaaaacaat tataataaat tatagctaca catctatttt taatttatac
     1561 aattaaagat ttattcgatt tattaaataa taaacattgc tactatggtt gttacaacca
     1621 atccggttat aactgggaca aagtttcgct ttgccaattc tattggacta accccacaaa
     1681 ttgccgctgc tgaaataaga ccccaaggaa ctagacagcc tccacctacc catataccac
     1741 tgatttgtcc taatgcagct aatgcaccaa cacttgcatt tatagcagtt ccaaatacag
     1801 aggcaagtga accaactaat gaaatcccag aaaatccaga accatcaagc ccagttattg
     1861 ctcccactac agtttctata cctgatacgg caaatttatt aagtggtatt gtttgagaaa
     1921 gagctatgcc tatatctgat aataaacctt gtgaacctgg tgctaatact tttccaaata
     1981 cacctgtcaa tggtgcaact tcacccatat agaaaaatgc tgctattggt ataattgctc
     2041 caaatatttt tatcccaaac acaaaaccat caattatatt ctcacataca ttttcaagac
     2101 tatctttgcc aaaatttata acatttataa ttattattaa aaatactgct gttccaccca
     2161 aaagagctga tgcatctcct ccttttaggt caaatacata catagcaatt atatcagcaa
     2221 gaaatcccag agctactaaa atagttgcta ttttagattt tccattgaac tcttctactt
     2281 cttcactttc aaaacctttt agttcttctc taaacattcc tttgttaata tctcttttta
     2341 atgtataaaa tgccatagat atggttacga ttcccatcac ccaaaacaaa atcataccat
     2401 catttataac atatgatact gcaacacctg ctgcaccagc tgttatactt ggagctcctt
     2461 gaataacaaa gtctgtagac aaggcaagac catgaccaaa taaatttaat gctactgcaa
     2521 caccaattgc tggtagccca gctctcatag caacaggtaa aaatacagct ccaactaagg
     2581 caacagctgg agttggccag aaaaaccaag aaagaactaa cataacaaat ccagttacaa
     2641 aaaatgctgt attagaattt tttataactc tcgaaaatgg tctcaccata tattgtatag
     2701 cattattggc ttctagggct ttagataatg caaccataat agctataatc aagataattg
     2761 gacccaactc atttaaagat ataataaaag agttaaacac tgctgttata gcaccaaaaa
     2821 tgtttttttc aaagaacaat cccataaaaa aaacacctaa tatacatggt actattgtat
     2881 cctttttcat aatcatagta attaatataa ttaccataaa tagaatgtaa atataatgtg
     2941 ctcctgtcaa aaccatatta ttatgtcccc tttttttaag ataataatat tatatggtct
     3001 tgtaagtaaa cttgttactt gcctagaggt acacaatatt gaacatccca taactaaagt
     3061 cacatgcttc ctgcttcata gaattttaca taagcaatta cacaatatat aatagcaact
     3121 acacaatata taatttaaat ttttatgatt cttttgctgg aatagatatt tcaatagcaa
     3181 attctttatc atctgaattt acatttatgc ttccattatg atatgcaact attttctcac
     3241 aattctttat accaattcca aaactttcta cattcttaga aaaattactt actctatttt
     3301 tttgtataat tagtaattta gaatcctcta ctttaattaa aaatactata ggcttatctt
     3361 tcctagcata ttttattata ttagaacata aattatcaaa tatccttcta aattgtgtaa
     3421 catctacata aatatgatat tggttataaa gttcattttc tattgttata tgaaatcctt
     3481 tctcctccaa tgtatatgaa ctatctaaaa gtaattgaaa aataaattca ttagcattta
     3541 ttttttcata cccttttgaa acttcctctc cagaattaac aaaatgcgta aaaaggtcat
     3601 ctgttaaatc ttttatttga tatgctttag accttacatt ttttatgtat tgttccaatt
     3661 gctcaggact attgtattta tggtctttaa tcaaatctaa ataacaaatt actgacgtta
     3721 atggagttct aacatcatgt gatatcgaag ttactatatc tttattttta ttcttcatct
     3781 ccctttcttt tataagttga ttctccaatg tatgactcat ctcattaata ctagatgcaa
     3841 gtgatgataa ttcatcatta ccttttactg ggattctata ctttaaactg ccactctcta
     3901 ctatctttat acccatttct attttcctta ggtactttat ctttctacta aacaacagta
     3961 aaaatgttat aataaatgta agtgccataa atgtaataga aattgcttct ataaaatttt
     4021 gaaatctaaa tctaaataaa gctactacaa ctatattagc tttaatatca gaaaattgta
     4081 tctcatagtt tctagccatg ggactgctat aaaactcatt ttcctcactc ttatcaggta
     4141 cttcattagg atagtataat gtatcataaa aaaccttatt tcctttatat atcttcatag
     4201 aaacaaacca attatcacta ttccattttg ctatatcatc tccatcatct gctcttatac
     4261 cattgttatt cacataagtt tgaaaattat ccataagttg atttacttct cttttaaaat
     4321 atactttccc ttctgataca tcttttacta tagattcaac agcagagtaa aatggtatat
     4381 ttaaaatgaa agccactgca gctgagatta ctacatatat caatagtata actcccaact
     4441 tagatggctt aattttgtta atcaatataa tacccctttc cccatgcggt ttttatatat
     4501 tgtggatttt tttggtcttt ttctaacttt tttcttaaat ttctaatatg aaccattatt
     4561 gtattatttg ctgtataaaa atactgctca tcccaaatag tttcatataa attttcagac
     4621 gaaaaaactt tttttctatt tttcaacata agaattaata tactatattc tatagttgtc
     4681 aaaaacacag gttctccatt tagtgtaaca tcttgagtat ctaaattgac acataactct
     4741 tttgttttta atatatttgg ctctctttcc ttttcctttc cctggtatat ataataacgt
     4801 ctaagtagag acttaaccct tgaaattagt tcagaatatg aaaaaggctt tgataaatag
     4861 tcatctcctc ctgctgaaaa tccaataact ttatcagagt cttgagtttt tgcagttaaa
     4921 aataatattg gtgctgtcgt tttttctcta atttcattac atgcaacaaa tcctgttttt
     4981 acaggcatta caacatccaa aataatcaaa tcaatcgtac tatctactaa gtttacagct
     5041 tcttctccat tcgttgccat aataacttca taaccttcac cagttaaaag tatttctacg
     5101 atttccctaa tctcaggatt atcatcagct actaaaattt tttttggaat ctctttattc
     5161 atcaccaagc tccttttttt gctatatttc ttaaatattt caaactatat aaaatattat
     5221 actttattag caaaagctta gcaatgtcac acgttttaat agtttctact cctgtaaaca
     5281 tgtttaaagc ttaattaaaa tatacacaca aattgacata ttaataaaaa taatctatat
     5341 aatattatct tacataagca acttttttca ctgcttttac tttttcagaa gattttacat
     5401 gtactactat acatatcaat ccacttatag ctacaaatgc ataaaaactt atccccctac
     5461 taagtaacat tgccggaact aataagtgac cagggaaaaa tatcttaaat aatgtcataa
     5521 aactattttc agaagctcca acagcccctg gcaatggtaa tgaagaaaca gcaatattaa
     5581 gcaaggattg tatcgctaat atctcaaata cagtatatgt tgatagatga aaagctttgt
     5641 atacaaagaa aggaactaaa aacatagctg ttatttgtac aatagttatg actaaaattt
     5701 ggactacaag ttttggattc tgctttatat attgagcacc tttaacatac tcttcaattt
     5761 gagatattat aacttttctt gaactttcaa catcttttat tattcttaac ttacctaata
     5821 aatttgttat tgatattata aaattgttta caagtttttt tgaaaatata attgcaatta
     5881 ctccaatgac aagagcaaca tttgttatta caccatatat tagtaaaatg ttcataccac
     5941 ttacattatt taatataaac tcacgttcca taatgaacat tattccacta tatgccagta
     6001 taactacttg gtgaataaca acaatgacta atagtataag tgaagaatat gatatattta
     6061 tatcgtcttt tttcatataa taaacttgtg ctggttgacc acctgatgct gaaggtgtaa
     6121 tagaactaaa ataaaatcca ataaaagcat atcccataca ttttaaaaga gtagtctttt
     6181 ggtttaaagc tttcatacct aagtatatat ttataccttc acaggcaaca tatataaaca
     6241 tcattgcaaa tccaattagt ataaacatag ggcttgcacc ttttaatgat tttatcaatg
     6301 attcaactga ctgccctcta aaaacaaaat atcctgttat gcccattagt aaaactaaaa
     6361 aagctacact acctaggcat ttatttttct tttcagtttt acttatcata actacatcct
     6421 ttctggtgtt acgaacttta aaccactggc tttaagtttt ggaatagcta tctttaaagc
     6481 ttcaatagta cgctcaggtg cacctactgc accacctgaa ttttcacctg catcatgtaa
     6541 acaaattata gaattttctt ttgttctact cattaacttg tttataataa tgtctactgt
     6601 actatctttc tcccaatctt ctgccattac atcccacaaa gtcattttta gattatattt
     6661 tttaacaaat gaatttgaaa atatattagt atgaccccat ggtggacgat agtagtttac
     6721 atctacacct aaatttttca ttatatttgt actttcaata aaatcttttt taacataatt
     6781 ataactataa agccatgcat ttctgtgttc caatgaatgt aaaccaacta tatgattttc
     6841 tctcaacatt ctttttataa tttcaggctc tttttcagca ttctttgcta ctacaaaaaa
     6901 catagcttgt atatcatttt cttttaacac atctagtaat ttttctgtat atctcttatc
     6961 tggaccatca tcaaatgtaa gtgcaatttc attttctcca gccatatttt ttaatacctc
     7021 tttatttaat agtttattat agtatgtagg tataattgaa tggactaaaa aaatcaatgc
     7081 aacaacgata attaaaccta ctacatacat tctctacacg ctccttgttc atcaagtgaa
     7141 aataaaatat tctctaaggt attattatca aattgttttt ttaattcttt catgttactg
     7201 ctcatttctt taagagtagc atcatcataa attaaatctt ttatctcatc tacatagtat
     7261 tttttatttt taccaagtat tctcccaatt tcattattta atataaaact agcattatct
     7321 atctcttgtt gtaaaaatgg attaaatgcc aatattggaa gttctgaata aattgtttca
     7381 aatagtgtta ttccacctgg tttagaaata ataaggtcag aatctttcat atatttatat
     7441 acttcattgg tgtaacctac tacctctata ttttcatatc taccatatag tttatagtac
     7501 atttttttat tattaccagt tattacagta gtttttactc cttctaaact atttaattct
     7561 ttgtaaaatt gttctgattt aggcagtaat cctaatcctc cacccattat aagaatattt
     7621 tttttagttg aatgatttac atgttctatt cttttaaatt ctttttttac tggaatacca
     7681 ttaactttaa tcttagattc atctattcct ttaaaaacaa gttcttcctt agtagattta
     7741 ctagctacta aataacagtc tgtattttta ctaatccatt ctgagtgact acttatatca
     7801 gttatacatg ttattaaagg aagatttgag ttatattttt ccttatatct tgatactaat
     7861 tgagaacaaa atggaaaagt tgaaataact actgttggtt gtacttcatg aagtaatgta
     7921 tctaatttat ttaaaaaata atctgaaaaa gtaaatttta tatctttctt tccattttca
     7981 gcacatttat aaaataaatt ataaagacta cttccccgat ttacaagaat agaaaaagtc
     8041 ttgtacattt tatctgaata gttaggcata atgtactcaa atatatcttt aattattatt
     8101 tctgaattat caaattttgc ttttatatct tcacttaaag aatttgatgc tgagtaatga
     8161 cccataccaa attttcctgt aagaattaat accttcataa gattccctcc tcgttaaagc
     8221 ttttcttttc atatgattac tatacttata agtatagact ttaaatcttt agaaaaacct
     8281 agtataattc ttaagaaatc ttaaagtttt aattaaaaaa tacaaagaga ccatctaaaa
     8341 atagagataa ccatattttt tattatttaa ataatacaaa tatgttcgat tccatacaaa
     8401 aaagtgcctc ataaactaaa aagttcattt tgagacacct tttttatatc taaatatatt
     8461 caaatacata cacaatcatt gtaattattt aaatgtattt tatttagttg ttctttataa
     8521 ttaatccata atattatatt cttcatataa aggatatttt tcagttaaac caacaaccaa
     8581 tgttctagcc ttagattctt catcataagt taaacatagg tctatagcct cagcgattat
     8641 tgccatatct tcttctttca tacctctagt agttacagct ggagtaccaa gtcttattcc
     8701 acttgtaaca agagcaccat ttgggtcaaa tggaattgta tttttattag ctgttatata
     8761 agcatcatcc aatctctttt ctgctgcttt accagttata tttttattag tcaaatctaa
     8821 tagtattaaa tgattatctg tacctccaga aatcaattta aaatctcttt taataagttc
     8881 ttcagctaag gcttttgcat ttttagcaac ttgtacctga tattctttaa actcatcact
     8941 taaagcttct ttaaagcaaa ctgccttaga agcaattata tgttcaagag gtccaccttg
     9001 aataccaggg aatattgctt tgtctatgtc tttagcatat ttttctttac aaagaataac
     9061 acccccacga ggtcctctta aagttttgtg agttgttgtt gtaacaaaat cagccacttc
     9121 acatggattt tggtgaagac cagcagccac aagacctgct atatgagcca tatctaccat
     9181 aagcaatgct ccaacctcat cagctatctc tctaaacttt ttaaaatcta tttctctagg
     9241 atatgcactt gctcctgcaa ctatcatttt aggttttact tcatgtgcta tctttctaac
     9301 ttcatcaaaa tctataagtc cactgtcttt atctatacca tattcataaa atttataata
     9361 ttgaccagaa atattaactg gagcaccatg agttaaatgt ccaccttgag ataaattcat
     9421 tcccataaca acatcacctg gttttaacat agcaaaatat acaccaatat ttgcatttgc
     9481 tccagagtga ggttgaacat tagcatgttc tgctccaaat atttttttga ttctatctat
     9541 tgcaagagtt tcaacttcat ctatgtattc acatccacca taatatcttt tgcctgagta
     9601 accttctgca tacttatttg taagatgact tcccatagtt tccattactg gaactgatac
     9661 tatgttttca gatgctatta attcaatatt tctttgttgt ctttttaact ctcttttcat
     9721 actttcataa atctcagggt ccgctttttt caagttctca aacatattac tcctcctagt
     9781 tattatttaa attttatttt tacagacatt tcataatttt cgtttttatc aatatgataa
     9841 tttattatta tagtccttta ccaattaata gtcaagtgtt ttgccatttt tccatattta
     9901 acatagttac tttcttt
//
LOCUS       DAMJYC010000128         9604 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJYC010000128 DAMJYC010000000
VERSION     DAMJYC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.134"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            122..793
                     /locus_tag="PC472_003503"
     CDS             122..793
                     /locus_tag="PC472_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728803.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            795..1703
                     /locus_tag="PC472_003504"
     CDS             795..1703
                     /locus_tag="PC472_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728804.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1780..2466
                     /locus_tag="PC472_003505"
     CDS             1780..2466
                     /locus_tag="PC472_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728805.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            2463..5018
                     /locus_tag="PC472_003506"
     CDS             2463..5018
                     /locus_tag="PC472_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2728806.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5212..6411)
                     /locus_tag="PC472_003507"
     CDS             complement(5212..6411)
                     /locus_tag="PC472_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2728807.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6530..7417
                     /locus_tag="PC472_003508"
     CDS             6530..7417
                     /locus_tag="PC472_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2728808.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7607..8164
                     /locus_tag="PC472_003509"
     CDS             7607..8164
                     /locus_tag="PC472_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728809.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8403..9263)
                     /locus_tag="PC472_003510"
     CDS             complement(8403..9263)
                     /locus_tag="PC472_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2728810.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3735 a         1160 c         1406 g         3303 t
ORIGIN      
        1 ttttattttg aaaacaaatt taaatacaat ctttttagtg aaataaataa aattaaaata
       61 tttttagtat gataatgcaa gaaaataatt atttatctaa aaaatctaaa ggagagtaaa
      121 attgaactta ttaattattg aagatgatat aaacttaaat gaaggtcttt tttatgcatt
      181 tgagaatgat ggatttaatg tatttaaggc ttatacaaaa caagaaggat taaatatatt
      241 taatagtaaa aatatagatt ttataatctt agactgcaat cttccagatg gagatggatt
      301 tgatgtatgt caaataataa gggaaaagtc agatatacca attataatgc ttactgcaag
      361 agactcagaa attgatgaag taaaaggatt agaaataggg cttgatgatt acattacaaa
      421 accattttct ttatctgtat taaaggcaag agtcaaagta gcaataagaa aaaaatcgaa
      481 taataaagta atatattcca atggaataaa attggaccaa aagttactaa aagtatataa
      541 aaataaagaa tgtttggaac ttagctctgt agaatataag cttctaagtt atctaattga
      601 aaataaaggt caaatattat taaaggaaca aattttacac catatttggg acagcgaaga
      661 aaattatgtt gatgataata ttgtatctgt aaatataaga agacttagag taaaagtaga
      721 ggatgaccca tctaatccaa aatatataaa aacagcttat ggaatgggtt atttatggaa
      781 tgaggtagaa taatatgttt atattagcct ttataatatg tacagttgtt atatttatag
      841 tatctactaa aataaatcga aaaagatatg atgaattaaa tttgatgtta aatcaaatat
      901 tggaaggtaa agaagttact tatccagata caaaggatac aagagcgtca aaaatttctc
      961 accaagtaaa aaaaattaag gatatgatag aaatagaggt agaacagtca aagtcagaaa
     1021 aagaagcgat taaaggttta atatcaaata tgtcacatca attaaaaact ccactttcaa
     1081 atataaccat ttattgtgaa cttctggaga atataaatat ttccacatta cagaagaagg
     1141 agtttttaca aaagatgaaa aatgaaactt ttaagattga ctggctgtta caatccttat
     1201 ttaaaatgac taaattagag gatggagtta tagaatttga agtagaagaa ctactaatta
     1261 aagatactct tattcaaagt atcagtacta tctttaataa agcagaagct aaaaatataa
     1321 ggataaatct cgaaccattt tcagacataa aattagttca taataaaaag tggaccatag
     1381 aagcaattgt aaatgtttta gaaaatgcaa taaaatactc accatcagat agcacgatta
     1441 ctatttctgt aataaaaatg gaactatata cgaagattac tattaaagat gagggaatag
     1501 gtattgatag tagagaacta aatgatattt ttaaaagatt ttacagaagt aaaaatgttg
     1561 aaaaccaaaa tggcacaggg attggtctat atcttacaag attaatttta gaaaaagaaa
     1621 atggaaacat tatagtagaa tctaaactag gaagtggatg ttgttttagt atcttcttac
     1681 agaattgtaa gagtttaaac taattttgta ggatgagtgt aattatcgtc acttacaata
     1741 ttttatagaa catatatctg tattgaaagg agaaattaaa tgagtatatt agtgacgaat
     1801 aatttagtaa aacactatgg aaaaggtgag actaaagtaa aagccttaaa tggagtaagt
     1861 atagaaattg aacgagatac atttactgct atagttggaa cttctggttc tggaaaaagc
     1921 acattgttaa atattatagg tggattagat aatccaacct caggtgatgt aataattaaa
     1981 ggaaagaaca tatctaaaat aggaaaaaaa gatttgacag tgtttagaag aagaaatata
     2041 ggatttatat ttcaaaacta cagtttaatg cccgtattaa acacttatga taatatagct
     2101 cttcctgtta cattagataa aggaaaccat atagaccatg aatatattga aatgctgatg
     2161 aagacattgg gaatttggga taagcgttta aaatttccaa gcgaactatc tggtggtcaa
     2221 caacagaggg tagcaattgc tagagctttg gcaaataaac ctgcgcttat attagcagat
     2281 gaaccaacag gaaatctcga tagtaaaact acaatggaag tggtatgttt attaaaagaa
     2341 agtagtgcaa aattccatca aacgatactt atggtaacac acaatgaaaa tatagcacaa
     2401 gtatgcgact caattatcca tattgaagat ggagttgtgg ttaatactgg tggtgaatta
     2461 ctatgatgtc tctagtaaaa ttttcaattc aatatataaa gcattataaa aaacaatcta
     2521 tatctatagt tttaagcata gtactatcag tagcattatt gacgggaatt ggttctttag
     2581 ttcatagtgc agacaaaagt agaattgaaa agataaggga agatagtgga gattatcatt
     2641 atttctataa agtagataag gaacaactta ataaaattaa agaaaataaa aaatcaaagg
     2701 aatacactgt agatagactt ggcgttacaa aacctaaaaa tcagatagat gaacctttta
     2761 ttatagaatt tttaaatgct gattcttcat atttagacat gacaggtaga aaattattaa
     2821 aaggaactct tcctagtaaa gatggagaga tagtacttga cacatattct ctaaacaatt
     2881 taaatgtaaa caaagaaatt ggaacagaaa tcaatttaga tggtaaaatc tataagttat
     2941 gtggtatttt atcaagtctt tcaaacccag aaactgctct ggagggattt gtaaacgatt
     3001 attttcagcc taaagaaaag gattcttata tagtttatgt aaaatatgat gaaaataaaa
     3061 atattaaaaa gcaaaattat aaatttatga aaaaattcaa catatcaaaa gaaaaaactt
     3121 atataaactg gtctctttca gttgaatttg gagtaaaacc accaaaaaat atagaaaaat
     3181 ataatatatt tgagttctta aaaaatttag aactaaatac aaatgcaatt atattgcttg
     3241 ttggagtgtt tggtgcagca gcagtatata gtattttcca tgtttcaata cttcagagaa
     3301 tatcacaata tggggtttta gaagtattag gcgctaataa taaacaactt ttatttctac
     3361 tatttttaga gttattttta ctttttattg ttggatttcc tataggttgc ttcttaggta
     3421 ttggagtagc gtctacaata tatgaacaat ttccacatat atttttgagt agtgatattg
     3481 ttccaggagc attttttata agtgaaaaat ctatttattt aggcttttta tttatcttat
     3541 tgttgttaat tttaattgct gtacgtgttg tacatcagtt gaataaatac tctagtatag
     3601 aatctatgaa aaactttaag attactaata agaaaaagag aatattagaa agtgtaaaac
     3661 actctaacat gacaaaagta ttatctcata aatacatgac tcaaaaaaag ggaatgttta
     3721 ttggaatttt gttttcatta tcacttggtg gaattatatt tctatgttct agttactcta
     3781 tacagcttac taaaaataat aatgaactta caatgaaagc tgatgatggt ttaaattctg
     3841 actaccaaat aactatgcaa acaacagatt ttgatgtagg tataccaaaa gataaaattt
     3901 caagattaaa caatgttgag ggaatatcaa aagtctatcc agtaagatat ttatttggag
     3961 ctatgcaaat taaagaagaa caactatttt ggaaaaattt ctttaaacct cttgaaaaag
     4021 attacaggat aaagactttt tttgatggta tttgtacaaa gcaaaatgaa ggtggctatc
     4081 ttttaaaaac aaatatttat ggttataaca atggtatgtt agaaggatta aattcttata
     4141 ttatagatgg ggagattaat acccatgata tggttaaaaa taataaggtt atagttagac
     4201 tacctatgga tggaactggg atgtatgatg cagttgatat aaatccagga gatacaataa
     4261 aagttaaagt gccaaagaca atgaaaccaa cagatgaaat tgttaaattt aaagaagaaa
     4321 ataagaatga ttatacaaca aaagaatttg tagttgctgc tactgtaaaa cgtgtgatgg
     4381 caaataacat atatttcatt ggtgattatg gtatggatat agttatgacg aatggtcaaa
     4441 tgaaaaataa ctttgatatc gaaaattata attcagtaag tataaaaaag acagaagata
     4501 gtaacagtga ggaattgtct gagaacataa aaagtgttgt aagtgatgta aaaagatgta
     4561 tcgtaacaga ttataccatt gctattgaaa aaaataatac ctacttaaat caaaaactct
     4621 tatttatata tggtattgta tttgtacttc ttgcaataag tttgttccat ataatcaata
     4681 cagtaagcta cttaattttt tcaagaagac atgagtttgg aattttaaga gctatgggta
     4741 ttacggataa taagttttta cttatgatga taagagaagg ttttttatat ggattctatg
     4801 caagtattat aatggtaatt ggaagtgtta ttggacaatt tatgatttat tttatggtaa
     4861 aacgtgtata tttatatata aatccaattc ttaaaataaa tacaccttta tacataggta
     4921 tgattatttt aaatatcact attagtatag ttgctgtaat tattccagta agacaaattt
     4981 tgaagagtga tattatttca gaaataaata agaattagag ttatataaat ttttaagatt
     5041 atataacttt ctgacaatct tgtttaaatt atttaaatat atcttatctg tgaacattaa
     5101 ataaacctct taataaatat aactcttgaa aagtcatatc tattaagagg ttttaataaa
     5161 ttttcatgtt aatatatata atccaaaaaa attaaatctt attaaaacat actattttgt
     5221 atagttatcg aaatatcctt gtatgtaaat aataggagta cccttatctc cacttccaga
     5281 tgttaaatct gctaaagaac ctataaggtc tgttaatctt cttggagtag taccttgaga
     5341 aaccatgttt cctgttaaat catcagattt attatctttt tcaactatgt attttgaaat
     5401 tgcttctttt aactcatcac cagataagtc agcaaaatca ttatctgcta agtattttaa
     5461 ttttacttcg tttggagttc cctctagccc ttttgtataa gcaggagata ctactgggtc
     5521 agctaattcc catattttac ctacagggtc tttaaaagct ccatctccat aaaccattac
     5581 ttctacatct ttccctgtta tttcttttat attaccttgg attttgttta caacttcatc
     5641 acaatttata gggaataatt ttactgtttc ttctgtagct ttgtttgaac ctaaaagtcc
     5701 atactggtca ttgtaaccac taccatctac acttgctgtc attatatcat caagtgaata
     5761 tactttttta gctccatttt gtcttaaaat tctctttgtt ctttgtcttg tatgtatatc
     5821 acaagttaaa atacttgttg tatagttaag aatagttttt ggattgtttg caaatactat
     5881 ttctacctct gcaccacaat ccttgattaa attcttataa taatcaacat aatctacacc
     5941 agtaaatgta tgcttgttat atccaaatag ttctctgtat ttttcttctg ttaaaacatc
     6001 tgaccaagga tttattccct tatcatcaag ttcatcaata cttattaagt gatttccaac
     6061 ttcatcagaa gggtagctta acattaatac gacttttcta catccttttg caattccttt
     6121 taaacatata gcaaaacgat ttctacttaa aattgggaat ataaccccaa cagtatcatc
     6181 tccaaattta tcttttacat cttttgcaat attgtcaacg tgagcataat ttccctgtgc
     6241 tctagctaca acagcttctg taacggcaat tacatctttg tccctaactt caaagttttc
     6301 actttttgac gcatttaaaa cagaatcaac aactatttta actaagtcat ctccttgtct
     6361 tataataggt gctctaaccc ctcttgaaac agttccaact aatctatcca ttatctaaaa
     6421 tcctcctaaa gaattaatat tgtcatatta gtaccatgta tattataatt caaatataat
     6481 tataagtaaa ataaataata ctaatatttg acataaggag taattatata tgaatataaa
     6541 acttgaactt tataaggttt ttaatgcagt tgtaaataat aagagttttt ctcttgctgc
     6601 taaggaattg tttatgtcac agccagctgt aagccagtca ataaagcaat tagaagaaca
     6661 attagatact ttattatttt atagaaacaa taagggagta aaattaactc cagaggggaa
     6721 aattctaagt gaacatgtca ctactgcatt aaagttaata tcatcaggag aagatagaat
     6781 aaataaattt aaaaaattag aatacggttc tctgaaaata ggagtaggag atacagctgc
     6841 acgttttttc ttgttaaaat atttagaaat ttttcataag aagtatccac atatacatgt
     6901 atctacaata aatagaacga gtagagaact tatatcttta ctcaaagatg ggaatataga
     6961 tatagcaatt ataaacatgc caatagaaga tgatacacta aatattgttg aatgtattga
     7021 aatacatgat atatttgtat gtgcaaatga ttacatagaa tacaaaggta ggaaaatatc
     7081 attggaagaa ttaaacacat tgcctttaat aatgctagaa aataaagcaa attcaagact
     7141 ttatgtaaat gaatattttt tatcaaaagg tataaaacta aacccggata tagaattggg
     7201 ttctcatgaa ttacttttag aatttgcgta tataaatctt ggagtgtctt gtgttataga
     7261 agaatttagc atagattatt tggaaaatga gaaattgttt aaattagata taaaagagcc
     7321 aatacctaag agaaatatag gttactgcca tttaaaagat atatctttat ccttagcaac
     7381 caaagaattt ttaagcatga tatctaataa tatataattt agaatacata tttcacaagt
     7441 aatttaatat aaaaatagag ataaattcgt aaatttgaaa gattcatata gtaattagtt
     7501 tttattaaaa ataaaaataa ggttaatttg agaacattta tataattaat atcgtatttt
     7561 aaatatgaac agtatattta gattaatgta taaaggggga ttagaaatgt ctgattctga
     7621 atatcctcaa atgaaaacct tagaaactag aaattgtata ctaagacctg catcattaga
     7681 tgatgttgaa gatttttttg actgttataa aaataaaata gtagtaaaac atttaccatt
     7741 tagtgagcat aaatctttag aagatacaag aaagtttata aaatcatttt ttctaaatcc
     7801 atataaaaaa gggaaaattg gccactttgc tatagtatat aaaagagaca ataaagttat
     7861 tgggaatatg gggtttaata atataaatcc taaagcatta gaagcagaaa taggtatatg
     7921 tataaatcca agctattggg gacatgattt tgctactgag ataacaaaaa gagtaataca
     7981 gtatggattt agagaactaa acttaaataa aataattgca ataacctatg aagaaaacca
     8041 aaattctaca aaatcattag atttattagg atttaaatgt acaggaaagt atatcaaaaa
     8101 aattcatact ggaaatactg tgaaaaatgt tccttgttat caatatgaat tgaaaaaaca
     8161 atagatataa tcatttaata tctatgctgg ttatttagta atgattgttt tctaaataaa
     8221 atttcaataa agtggttgcc tcaaaatata gataatcctg tttttattgt taaaatactg
     8281 aaaatgcatt tgattttata caaaaaagag tgtctcatga actaaaaagt ttatgagaca
     8341 ctctttttga ataaatcgta ttttaattaa aatagtatat atacttaaaa tattaatttt
     8401 tattagttct ctaaattttt aaatccttct ccaagaacct catgtacatc tgtaacaata
     8461 acaaatgcag tagggtcagt tcttttaact aatctcttta gataaagttc ttgtttttta
     8521 gaaactacga ctaatagaac ttctttattt tctctagtgt atccaccttt accatcaaga
     8581 atagtaagac ctctatcaag ttcttcagtt atagcttgtc ttaaactttc aggctcatca
     8641 gagataataa aaaatgcttt tgaatggtca aatccttcca ttatcatatc tgctattttt
     8701 attaatacac aaagtgctat accagagtaa agagcagttt ctaaactacg atttactata
     8761 ccagaagata taacaaccat tccatctagg catgacatga attttgtagc ttttattcct
     8821 gggaatttct tactaagtat gagtgcaatt aagtctgttc ctccagtaga agcatctgat
     8881 ttaaacatta ttcctaaacc tataccaact aggattcctc ctgcaataga agacaataat
     8941 aagtcattag taaatcttag ttttgataat gggtctgtta attgaactat agctgaaaat
     9001 acaactgttc caaataaagt ttttaatgaa ttttttgtac ccataattct aaaagcaagt
     9061 attactaatg ggacacctat aattaacatg atagctgata ctggaagtcc tgtaaccttg
     9121 tttagtacta atgatagacc actaagtcct ccaggagcaa ttgtatgtgg ttttaaaaac
     9181 atatttatac caatactcat tgatatacaa ccaaaaaata gtccaagcat ttcaagtaaa
     9241 attggcgact tttgtttttt catataaaca cctccatgaa cttgtcacaa tttaatatta
     9301 taacatattt gaccagttta tattatataa aatttcttga tataagcaat ttcaagtatt
     9361 tcattattct taatattttt gaatttttat atagtgtttt catattttta ctgatgttgt
     9421 tactctccta tgaagttttt atatattata atttttttat tcttttataa agtatcccct
     9481 ataaagatgg ttctatgaaa attttctcta tactgtttaa cgtatataga gattttgtgt
     9541 ataattataa ggtatttaat ggaaaaattt atcttacttt tatatgttat caaaaggatt
     9601 tatt
//
LOCUS       DAMJYC010000129         9474 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJYC010000129 DAMJYC010000000
VERSION     DAMJYC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.135"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(126..779)
                     /locus_tag="PC472_003511"
     CDS             complement(126..779)
                     /locus_tag="PC472_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2728811.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1024..2889)
                     /locus_tag="PC472_003512"
     CDS             complement(1024..2889)
                     /locus_tag="PC472_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2728812.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKNADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2894..5314)
                     /locus_tag="PC472_003513"
     CDS             complement(2894..5314)
                     /locus_tag="PC472_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2728813.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5328..6770)
                     /gene="glgA"
                     /locus_tag="PC472_003514"
     CDS             complement(5328..6770)
                     /gene="glgA"
                     /locus_tag="PC472_003514"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2728814.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSIN
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6799..7929)
                     /gene="glgD"
                     /locus_tag="PC472_003515"
     CDS             complement(6799..7929)
                     /gene="glgD"
                     /locus_tag="PC472_003515"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2728815.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7931..9091)
                     /locus_tag="PC472_003516"
     CDS             complement(7931..9091)
                     /locus_tag="PC472_003516"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2728816.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3006 a         1725 c          919 g         3824 t
ORIGIN      
        1 taaatacatt attctatcgt agtgccttag agtttttatt tgtatttaaa gttaaattta
       61 ttcttgtttt tcctttaata tttaatagta aaacaacaaa attataaaaa aaataaagag
      121 aaactttata aaagcttctc ttttttcatc tctgctatag gtatcgtttt ccaatgttct
      181 ctcaaaactc tttcaacatt ttctaaatca ttctccctca tagattttac tacttcaata
      241 tgttgattat tgctgtatgt caacatttta tataactcat cttcattatc actagtatat
      301 gactttgaca taaaattata tcttaaagag ttcaatagat ttactaacac ctgattatct
      361 gatttagtta tgtatatatc gtgaaattca ttacttaatt ttaaatagct attgtaattt
      421 ttatacttta tagagaccgc catcatttct gttaattctt ccatttttac taggtctgat
      481 tcagttaaat tttgtaatgc tgaagaaccg gctaacgcat ccaatactcc aattacggaa
      541 taggtgtcta atttctcctt ttcctgaaat tcttttacta caaatccctt attaggtatt
      601 ttttctagta aattttcacc tgctaaccta gttaatgctt ctctcgctgg tgttctacta
      661 actccgatag atttacaaat ctcagattca gtaattctct gattagcttc atattctcca
      721 ttttggattt tctttgatat atagtcatat acttgagttg taagtggatc taccttcata
      781 agacgttcct ccatattaag attataaaag ttaaatctcc ctacaacttt ggtagagagt
      841 tcagttttat cttactaatt tatataatat cacaattatg atatttttta tacttctttg
      901 taatattttg aaatactaaa attcttatga taatttatac aaaaaaagta gaaaaagttt
      961 tgataaaaca aaactctttc cacattcacc ttattttcta tttatttttc taaaaaaaga
     1021 tatttaattt actctaaaaa ttctaaatga tttagagtct aaatctactt tcaactcatt
     1081 attttcaatt tgtatagtac tatttgcatt tttaagacta tactttatag gtatttcctc
     1141 aataaaacta ctctctatac ataatgatat actttccaca ttttcacttc tatttactaa
     1201 aattaacatt ctatcattat tttcattaac tctaataaaa gaaaatacag aatcattatt
     1261 agtatatata aattctgtat ttccagaact taaaagttta ttcttatttc taatttttat
     1321 aacatctcta taaaaattaa gcatatcttc gtcttcattt ttccatggat aagttcttct
     1381 attatctggg tcaacacctc cacaaagacc tgcttcatct ccataatata tatatggtac
     1441 tccttcaaat gtcatctgta tagctacagc aagcttcact aaatctacat catcatttaa
     1501 ttccgtttta gctcttgtaa catcatgtgt tccaattaaa tttaaattag acctaaatga
     1561 ttcttttgga taattttctt taatctgcat atatctatta caaagttcat atgaattaat
     1621 ttctcctttt aagaaagaaa acatgttatt tctaaaagga taacccataa cagaatctaa
     1681 ttcttcacct aataaataac ttcttctctg accatagcta atcttatttg atgcatcttc
     1741 ccatacttca cctataagaa ttgagtcatc atctgcattt tttaattcct tttttaactc
     1801 tcttataaac ttagttggta attcgtctgc aacatctagt ctccagccct taatacccat
     1861 atttacccat ttatttataa cgctatcatt gtcatatatt atataatcca tatagctatg
     1921 ttctaattca tttatatttg gcaatgtctt aacatcccac caacttttat atttttgagg
     1981 aaattcttca aacatatacc atgaataata tggagattct ttagattgat atgcccctaa
     2041 actattatag tttccataca tattaaaata tttactatct gctccagtat gactaaaaac
     2101 tccatctaat actattgata tacctttttc ttttgcttta tcaataagtt ctttaaatgt
     2161 atcttcatct ccaaacattg ggtcaatttt tttatagtca cctgtatcat acttatgatt
     2221 actagaggct tcaaatatag ggcttagata tagtatactt acacctaatt tttttaaata
     2281 tccaagttta ttgattatcc ctcttaaatt tccaccatgg aaatcccatc ttataacatc
     2341 cccttgacta tcctttatgt acataggtgt atcttcccaa tttccatata tgaaactatt
     2401 ttttttagga ttatcaacct taccactacg atttccatta ttgaatctat ctacaaatat
     2461 atgataaagt attccttctt tgtaccatgt tggtacttta aaatctttat aaacagtgat
     2521 ttgatattta ttaatatcac tataattata ttcacaagaa tatccattat ctctgttttt
     2581 tccataaaat aaagtcttta tttgaccatc catttcaaca tcaactttaa agtaataaaa
     2641 ataaacacct gtaccatcaa acttttcaat cttaccacaa aagcactttc caagtttatc
     2701 attatctaat tcttcattta aagttattgt tctcatttct tcttctctat ttatttctag
     2761 ggaaatagac ttgatattgt atccctcatt tactttaaca caaatggtca attcctcatc
     2821 aaattttaaa gctccaaacg gagccttaaa atttttatcc caagagttat attctattac
     2881 actttgcatt taatcacacc cttttaaacc aaatgtcttt tgcatactca gaaattgttc
     2941 tatcagaaga aaaaaatcca gagttagcta tattaactaa agacatttta ttccattttt
     3001 ctttatctct atataacctg tttatatctg cttgagcttg accataattt tcaaaatctc
     3061 tcaatacaaa atactcatca ttgtaagtag tgagtgaatt ataaatactt cttccttctt
     3121 cgccaagatt aggaataaat ccatttatta aatcatctac tacccttttt atatccatat
     3181 tagaatgata taggtcaagt gaagaatatc caccatattt attataattt agtacttgtt
     3241 ctgcactaag tccaaataaa aacatatttt ctttaccaac ctgttcacat atctctacat
     3301 ttgcaccgtc aagtgtagcc aaagttatag caccattcat cataaacttc atattacttg
     3361 ttccagatgc ttccttagtt gtagttgaaa tttgctcact aacatttgct gctggtatta
     3421 ttatttctgc taatgatact ccataattct ctaaaaatac tacttttaac ttgtccttta
     3481 ctctaacatc attatttata gtacttgcaa cagaatttat aaatttaatt atacatttag
     3541 ccaaataata tcctggtgct gcttttgcgc caaatataaa agttcttggg tcaagattca
     3601 aattaggatt atcaagtaat tcatgataca tatgaagtac attaaatata ttcagtaact
     3661 gtcttttata cgcatgcaat ctcttaactt gaacatcaaa tatcgaactt gggtctacat
     3721 ttaaatcgta tttatctttt ataaactttg ctagatttgc tttattattg tattttatat
     3781 catcaagtct ttgtaaaaca gaggaatcat ttttaaattt ttcaatattt tttaattgta
     3841 atgtatcttt cttccatgaa tcaccaatta aatctgtaat taaattgcta agttgaggat
     3901 ttgaacttat aagccatcgc ctatgtgcaa ttccattagt cttattatta aatttatttg
     3961 gttcatcttg atagaaatct tttaatacct ctgtttcaag aatatgagta tgaagcttag
     4021 caactccatt tacactatga cttgtgacaa tacataagtt tgccatattg atattgtcac
     4081 agtctattat actcattctt tttattctat cttggtcata tcctttatta tttaattcct
     4141 ctacatatct tctatttatt tcttctatta tcatataaat tcttggtaat agctctttca
     4201 tcatacttac aggccatttt tccattgctt ctgacattat agtatgatta gtataggaca
     4261 ctgttttttt agttatttcc catgcttcat cccaagataa attttcttca tcaagcaaaa
     4321 ttctcataag ttctggtatg cataaggtag gatgtgtatc atttatatga attgctacct
     4381 tatcgaataa attattaata tttaatttgt tctttttata ttttcttatt atatcttgta
     4441 tccctgcact tacaaaaaag tattcttgtt tcaacctcaa caacttacct gcataatttg
     4501 aatcatcagg atataatacc tgtgatattt cctcagtata atacttatgt tttaaagcct
     4561 cttcatagct accagaataa tttagagcat ttgaggtaag ttcaccaaaa tctctctttg
     4621 gtatttcact cttaaacaat cttaaagtat ttatacattg gttttggtat ccgattattg
     4681 gtatatcata aggcattgcc attacaggaa ggtagttttt atgaattact tttaaatgac
     4741 taccttcctt gattaattct acctcaccac caaacttcac cattgttgct tcatttggtc
     4801 ttacagtttc ccatgcatat cgtccttctg taagccagtt atcaggcact tctacctgat
     4861 aaccatttac aaatttttgt tcaaacagac catatttata ccttattcca tatccttgac
     4921 cacttatgtt caaagatgcc atagaatcta aaaagcaagc tgctaatcta ccaagtccac
     4981 catttcctaa ggctgggtct acttcagctt caattaaatc atccaagttg attcctaatt
     5041 cagataaacc ttctctaact tcttcttcaa tatttaaatt taataaatta gattttaact
     5101 gtctccctat taaaaattct aaactaaaat aataaacttc ttttttctta tctaactttg
     5161 tagcaaccca tttttttgct atctcatctt tcaatagact acataataca tttaacaatt
     5221 gttcatcagt agcttccttg atagattgtg catataagct atacattttt acttcaaatt
     5281 tttgcttgaa tttctttttg cttattgtta ccaaaaaaac cactccttta atcacatatg
     5341 tctctatatg tttctatata ctccttagct gattttttcc aactattatc tgttttcatt
     5401 gcattttcaa ctaatttaat ccatttttct ttgtccttaa atactttgat tgcatttttc
     5461 aaacagaaaa acatctcatg agcattgtaa tttttaaaac taaatccatt gccttctcca
     5521 gtaaatttat tataaggtgt tactgtatct ctaagccctc cagtctctct aactattggt
     5581 aaagaaccat atctcatagc taacatttga cctataccac aaggctcaaa taatgatggc
     5641 attaagaaca tatcacttgc agcatatatc tgttgagcca atgatgcatt aaaagtaatt
     5701 ctagctgata ctttatctga aaaattagaa tcgtaatagt tgaacattga ttgatattga
     5761 tgttctccag ttcctaaaac gacaatctgt aaatcttctc tcataagttc tggcatcatg
     5821 tatgctatta aatcaaatcc tttttgagaa actaatctcg aaactatacc tatcaacgga
     5881 atagatgaat ctttttttag tcccaatata tcttgtaatc tcaatttatt ttctactttt
     5941 ttgttaattg agtctacatc ataatgaaca aatatatctt tatcttttgc agggtcattt
     6001 aaatcataat ctataccatt taatatacct tttaattttc ctgactcttt tctaagtaat
     6061 ccatctaatt gttcgccata aaaagatgtt tgtatttcat ttgcataagt aggactaaca
     6121 gttataattt tatctgcaaa atttatacca gctttcataa aatttattga gccataatac
     6181 tctatatctc cattatcaaa gtatttttgt ggtaaatcca atatatcata taaaacatgc
     6241 ttatcaaata caccttgata ttggagatta tgtatagtat acatagtttt tatattttta
     6301 tacccctcaa gggttgcata tctttctttt agaattaatg gtaacatccc tgtatgccaa
     6361 tcatttatat gtattacatc tggataaaaa tcaattctac ttatagcttc taaaactgca
     6421 tctgtaaaga aagtatatct ttctgcatca tctccatatc catagatact ctttctttca
     6481 tcttctcttc taaaataaaa ttcattatct ataaagtaaa attttacacc atctaattcc
     6541 atttctaaaa gaccacagta ttgatttctc catccaacac gtacactaaa cacagctatt
     6601 tcttttagtt gatcttttaa ataacttggt atagttgaat atttgggcat tattaccctt
     6661 acatctacac cttctttttt taattcttta ggcaatgcat aagaaacatc tcctaatcct
     6721 ccagtttttg caaagggcca acactctgct gttacataaa aaaccttcat tagtcattcc
     6781 cccttctaaa aaatttattt atatatttgt attcttttcc actacaagag gatatttttc
     6841 atctcctctt aattctttcc catttgatac actcgtattt ttatctaata ttgcattata
     6901 tagttttgca tttgacttta ttacacaatt ttgcattact attgagttct ctataactgt
     6961 tcctttttca acatttacct ttctaaatat gatactatct tttacagtac cttctattaa
     7021 acaaccagat gctataaagc tattttctac tttagcacta tctgtataaa ttgtaggtga
     7081 ttcattcttt tcttttgtaa atatctttct ttcagaatat aataattcat ctgcaacttc
     7141 taaatttaat aaatctttgc tcatttcaaa atatgattca acagaattta cacattttag
     7201 atatcctgta tattcatagg ctcctatttt catgttttct gcttcttcaa taataaattc
     7261 ttctaaataa caataattgc ctttatttac aatgttatat atacattcaa caaaatcttc
     7321 acgcttcata atatatgtct ccatacatat gtttgctcta gggaacttac ctaaattatt
     7381 tcctacattt attaccctat tgttactatc taaatttaga gttaagttac cagcaaaatc
     7441 tttatttgca ttatttacat ttttatatat tatagttata tcattatgag attttttatg
     7501 gtattccaat gcttgagaat aatttaaatt gcatagcata tagcttggag ctattaaaat
     7561 atattcttct tcactctttt ctatataatc tagattagct aagatagtat atatatcacc
     7621 ttctctatgt gagtaagtat ttctatactt agtgttttct ggactaaaaa ttgataggcc
     7681 accttttttt ctacttaaat cccaatcttt accactacct atatggtctg ttaatgatct
     7741 gtatttttct tttgcgaata ttccaacttt agtgattcct gaatttatca tattagataa
     7801 agcaaagtct attattctat atcttccagc tattggtgta gatgctattg gtcttccata
     7861 gttaagtttg tttattgctg ggtctccttt tttgtttaaa tttattatcc ctaaacattc
     7921 atttctcatc ttaaagacct ccttctatta atacgcatct aggttcgata ttttcatact
     7981 cagaaactac atttatttca ttatttacat tctttatctg aacattatca tttatagttg
     8041 cacactcacc tatcatagct ttttctatag ttacattttc acctattaca acatttggca
     8101 ttattactga atcaattact ttacttcctt taccaacaac aactccatgt gataatacag
     8161 agttacctac ttcacctaaa attctacaac catcagctat cattgatcta tgaacatttg
     8221 catttttacc tatatattgt ggaggcattg ccatagtatt tgtatatatc ttccacttag
     8281 ggtctgctaa atcaagtgtt tctggagatt ttattatatc catattagca tcccatagac
     8341 tttgtatagt tcctacatct ctccagtatc ctttaaatgg ataagcatac attccaacat
     8401 tatcttctaa cattttaggt attaaatttt ttccaaaatc atcataattt atttctggat
     8461 ttttttctgc ttccttaaaa taatttctca acattttcca atcaaatatg tatactccca
     8521 tagatgccaa attgctctta ggttcacttg gtttttcttc aaattcatag atacttgaat
     8581 cttcatttgt attcattatc ccaaatcttg aagcttcatc ccaaggaact tcaatcactg
     8641 ctattgtagc tttagagcct ttttccttat gaaattttaa cattttgcta taatccatct
     8701 tgtatatatg gtcaccagat aatatcaata catattctgg attataggta tctacaaagt
     8761 ccatattttg atatatcgca tgtgctgtac cattatacca atttccttct ttttcattca
     8821 taaatggttg taaaacatag acaccaccat ttattctgtc caaatcccaa tgacttccca
     8881 tgcctatatg tgaatttaat atcaacggtc tatattgagt taaaacacct acagtatcaa
     8941 taccagagtt tgaacaattg cttaacacaa aatctataat tctatactta cctccaaatg
     9001 atacagctgg tttggcaatt ctttttgtaa aaacacctaa tcttgaacct tgccctcctg
     9061 ccaaaatcat agctaacatc tcttttttca tttaatgaca ctcccctcat ctagtaatta
     9121 atatttataa ttgcattttt acttttataa actttatcag tcaataaatg tcgtgtataa
     9181 atgttgctta aataatacct taataaaaat aaaataatat caactataca attgatataa
     9241 tgtatacccc ataatggact taccgacgta tacattattt tccataagca ctatagtatt
     9301 tattaatatt caattatcgg ttaaaaaata atacaaattt tattttataa atataaaaac
     9361 attttttata aatcccttgt gtcatataaa catgcttatt ttttaatttg ttaaattcgt
     9421 aatagcaaaa tataatctaa tatttgttaa ataattatcc aaaaaaaatc aagt
//
LOCUS       DAMJYC010000130         9384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJYC010000130 DAMJYC010000000
VERSION     DAMJYC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.136"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            393..1598
                     /locus_tag="PC472_003517"
     CDS             393..1598
                     /locus_tag="PC472_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2728817.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTNRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="PC472_003518"
     CDS             complement(1669..1890)
                     /locus_tag="PC472_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728818.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC472_003519"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC472_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2728819.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="PC472_003520"
     CDS             complement(2643..2993)
                     /locus_tag="PC472_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2728820.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC472_003521"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC472_003521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2728821.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="PC472_003522"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC472_003522"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="PC472_003523"
     tRNA            complement(5068..5159)
                     /locus_tag="PC472_003523"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="PC472_003524"
     CDS             complement(5160..5615)
                     /locus_tag="PC472_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2728822.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC472_003525"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC472_003525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2728823.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="PC472_003526"
     CDS             complement(7666..8547)
                     /locus_tag="PC472_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2728824.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="PC472_003527"
     CDS             complement(8559..9068)
                     /locus_tag="PC472_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2728825.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNVYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9195..9311)
                     /gene="rrf"
                     /locus_tag="PC472_003528"
     rRNA            complement(9195..9311)
                     /gene="rrf"
                     /locus_tag="PC472_003528"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2999 a         1639 c         1002 g         3744 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttaattttt ttatttatca tgtattcttt atctttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt gtcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa gcttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac tgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaagatattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactaat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatgatact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctacaataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaacaca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 tatcaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aaaagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctacatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tattgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatcctcta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta agaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaaatata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaaca ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta caatgacatc ttttataata ccacctaaat ctacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaaa gactacgtgg ctacgtccta ctctcccagg cagtttccca ccaagtacca
     9241 tcagcgctag agagcttaac ttctgtgttc ggaatgggaa caggtgtatc ctctctgcta
     9301 ttgtaaccac ataatcttta tgcttaatat ttctattaag tttttaataa atttatagaa
     9361 tctagtaata ctagtttctt taga
//
LOCUS       DAMJYC010000131         9309 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJYC010000131 DAMJYC010000000
VERSION     DAMJYC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.137"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            173..1261
                     /locus_tag="PC472_003529"
     CDS             173..1261
                     /locus_tag="PC472_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2728826.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1289..2080
                     /locus_tag="PC472_003530"
     CDS             1289..2080
                     /locus_tag="PC472_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2728827.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2195..2749)
                     /locus_tag="PC472_003531"
     CDS             complement(2195..2749)
                     /locus_tag="PC472_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2728828.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2945..4147
                     /locus_tag="PC472_003532"
     CDS             2945..4147
                     /locus_tag="PC472_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2728829.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4204..5184
                     /locus_tag="PC472_003533"
     CDS             4204..5184
                     /locus_tag="PC472_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2728830.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5432..6385
                     /locus_tag="PC472_003534"
     CDS             5432..6385
                     /locus_tag="PC472_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2728831.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6375..7331
                     /locus_tag="PC472_003535"
     CDS             6375..7331
                     /locus_tag="PC472_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2728832.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7328..8083
                     /locus_tag="PC472_003536"
     CDS             7328..8083
                     /locus_tag="PC472_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2728833.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            8175..9140
                     /locus_tag="PC472_003537"
     CDS             8175..9140
                     /locus_tag="PC472_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2728834.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSGNSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDGKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         3661 a          938 c         1557 g         3153 t
ORIGIN      
        1 atttttctaa gtgttattta tttgtagagc tgaataaaag tgaaaattct tatctagaat
       61 ttattgatat aattaaaaaa acaagtataa aagaattaac tatagtatgt tgtagctaaa
      121 gtgttttaac aggataaaat atatcacgat tttaagatag gagttgagtc atatgagaga
      181 ttttagcacg tattctaaag atgatgttat ttttttatta aaagatatat caaatttaat
      241 tgaagaagaa ggaaataaag aaagagaatt aaaaatacaa aatggtagac attattcaga
      301 aatgatacca atagagtatg aagttagtga agattatctg aatttatatc atgagaaact
      361 aagagaaaat aaagataaac ttgcattttc tataggagtt atgtgtgaaa aaataattaa
      421 aaaaaatgga aatgatgtag tactagtttc acttgctaga gcaggaacac ctattggtat
      481 tttggcaaaa agatatataa gaagcaagta taatgtaagt ttacctcact atacaatctc
      541 tataataaga gataagggaa tagatatgaa tgcaattgaa tatatagtaa agaatcatcc
      601 gagctcaaaa attcaatttt tagatggatg gactggtaaa ggaactatat caaaagaact
      661 tgaaaaagca tgtgatgaac ttaatgttaa atttaaaaaa tattttgact caacacttgc
      721 tgtattagct gaccctgctg gatattcagg actttatgga attagagaag atttcttaat
      781 accaagtgca tgtttaaatt caacagtttc tggtttagta agtagaactg tactaagaga
      841 ggatttaatt agtaaaaatg actttcatgg agctaagttt tatagacatt taaaagataa
      901 agatgaatct atgaactata tcgaaactat agaagaatgt tttaaaaatc aatttaagaa
      961 tatatctgat gaagttgaaa actgggaaag tgacattata acaagagatg gatactttga
     1021 tgttttaaat ataaaagaaa agtataacat aacagatatt aactttatca aaccaggtgt
     1081 aggggaaacg actagggttt tacttagaag agttccgtat aagattcttg ttaaagactt
     1141 aaatgataaa tctttagacc atatattcat attagctaag gagaaaaatg ttgaagttga
     1201 acaaatggat ttaaaagcct ataaatgttg tggaataata aaaaatatga aagatattta
     1261 ggaaatatat tagacaggag aatattgaat gattgttttt tcagatttag acaggagtat
     1321 catttactcc aataaatttt taaatgctga tagtaaatat gctaatatag aaatttatag
     1381 ggaaaaagaa atatcttata tatctttaga tactataaat ttaataaagc aaatacaata
     1441 ttatggtatg tttataccta caactactag aacagtggag caatttaagc gtatagaatt
     1501 taataaatat gggatttatt ttccttggag tataacttca aatggaggag ttatacttaa
     1561 agataatgaa atattgaaat catggagtga aaaaatagat aaactaaaat ctgattatga
     1621 gcccattgaa agtatgattc ataaatttaa agactatttg aatgtagatg gaataactaa
     1681 ttttaaagtt gcagaagata cattttttta tatagttgtt gatttatcaa gatttaattt
     1741 ggattcaatc aaagaatata caaatatatt ggaatcaaaa aattggaagt tttatgtgag
     1801 tggcagaaaa atatatttta taccaaaaga aatttcaaaa gaaaatgcta taaaatactt
     1861 gactaaagaa ttaggaatag aatattttta tgcagttgga gattctatta tggactatgg
     1921 tatgcttaat atctcaaata aatcctatgt attgaagcat ggtgatataa ataaaaatga
     1981 aatagaaaat tcatttatat caagttcttt caatggaatg agtggaactg aagagatact
     2041 atctaacatt ttagatgaga actgcctact taatatataa tgactgatat ataaataata
     2101 aactagaaaa atctgaaata aattatagta aagtttatca tactaattta tttcagattt
     2161 ttatcaattt atatttaagt aagaactcaa tatgttaaaa tgaaggtgga gtcattgccc
     2221 agactgagat acatacctca tcatcataat tttcatacac atgtggtata gtactgttaa
     2281 aataaaaact atctccttcc tccaatagat attccttatt tccaagtgtt atcttcattt
     2341 taccttttat tatatagcca cattcttctc cttcgtgtgt cacaaattca ctgtttttgt
     2401 gtgtatgacc atctagtgta acaagtataa attctatttt tttcccagat aaattaggaa
     2461 caagaagttc gtaaaatttt gtagaatcat tagttactat tttttttcga ttatttttct
     2521 ttactactat atcagagtct tctcttccaa tctcttcaaa aaaataacct atatttatat
     2581 caagagcttt tgatattttc cacattgcta caatagatgg aacaaccata ttacgctcaa
     2641 tttgacttat taagcctgta cttaaatctg aattttttgc taaagtagag atacttatat
     2701 ttttagattt tctaagagat ctaattttgc ttccaacatc tatattcatt agatacaaac
     2761 cccctcagac atgattataa catataattt ataaagatta ataaaataaa aaaaaagttt
     2821 tacaatacaa gtatttttta tgctatattg aattttctga aaagatgcat tataatagta
     2881 ttaatattca atatattgaa tattgataac gatattttaa tttaatgaaa tggaggtaat
     2941 taagatgaag agttttgtaa gtcctgataa tatttgggta ttatgggcag ttgtaactgg
     3001 atgggcagca tttagtattt atttggaaca aaagtacaat tgggcatcaa aagtgtcggg
     3061 agcaataata gctcttgtag gagcaatgtt attatcaaat ctaaatatta taccagtaga
     3121 gtctgttgta tatgaccaag tatggggata tgttgttcct ttagccatag ctttattgtt
     3181 atatcaatgt aacattaaaa agatatggaa agaaagtgga agattgctca ttatatttct
     3241 tgtaggttct gtaggaactg tacttggagc tatgattgga tttttagcat taaaaaatgt
     3301 agtaccagat ttaaatattg ttgcagctat gatgacaggt tcttatatag gtggaaatgt
     3361 aaactttgct gctatgtcag gggcgtttga tgcgccagga gaattagtat cagcaacagt
     3421 tgtagcagat aatcttttaa tggcattata tttctttgtc ttaatagcta taccatcaat
     3481 tggatttttt agaaaacatt ttaaacatcc acatgtagat gaaatggaaa gtataggtat
     3541 aactgaagga gaaacagtag cagcaaatta ttggggaaga aaagaaatat ctttaaaaga
     3601 tatagcaata gccattggta gcgcttttat aattgtcgca gtatctgtag aactatcaac
     3661 ttggtttaaa gctataatac cattatcaaa tcctttttta gcaatgttaa atactttatt
     3721 tggaaatcaa tatctaataa taactacttt gactatgttg tttgccacat ttatgcctaa
     3781 tttctttggt gatataaaag gagcacagga attgggtaca tttttaatat atatattctt
     3841 tgttgtagta ggtgttcctg cttcaataac attaataatt caaaaatcac cattactact
     3901 tttatactgc ggaataatgg tactaataaa catgctagtt acatttatag ttgcaaaaat
     3961 atttaagttt agtttagaag aagctattct tgcatctaat gcaaatattg gtggtcctac
     4021 aacagcagca gctatggcaa tatcaaaagg atggtctaag ctagtagggc caatacttat
     4081 agttggaaca tttggataca tagtcggtaa ttattttggt cttttagtag gaaatatttt
     4141 gatataattt tacgtgaaaa aaagttgttt tttagaaaga tttttattta attggaggtt
     4201 gttatgattt tcgatggaca ttcagatact tgggcagatg taacaataag aaggcttaac
     4261 ggggaaaaag atgtatttag aagaatccat cttgataatt ttaaaagagc aaatatagaa
     4321 ggagctattt ttgtaatatg gatagaccca ccatatgata aagagcctga aaaaagagtt
     4381 aaacaaatta taagctctat aaaagatgaa attgaagata tgaaagatgt tgtaaacata
     4441 gtaaaaaaat attcagatat agataaaggt ataagtgaaa acaaaataaa tattttaatt
     4501 ggtcttgaag gattaagcca tattggaaga aatatagatt tactagatta tttttataaa
     4561 gaagttggtg caagacatgc atcattaact tggaatgaac agaatgaact tgctacagga
     4621 tgtttagggg atgaaaatcg tggtttaact gaaataggta aaaaagcagt caataaaatg
     4681 gaaaaattgg gcatgatagt agatgtgtca catttaaatg aaaaatcatt ttgggattta
     4741 atgtctgttg caagtaagcc agttatagct tctcattcta atgctaggag tttatgtgat
     4801 gctaaaagaa atcttacaga taatcaatta aaagaaattt caaatacagg tggattagtt
     4861 ggaataaata gttttagagg atttactaat agtaatccta agaagcaaga tttaatacat
     4921 ttggttgacc atatagacta tatggtaaat cttataggta tagaacatgt atcttttggt
     4981 tttgatttta gtgattactt agggaaagag actataggtt cttttactgg aaatgctagt
     5041 gtgccaagtg tagatggtct tgaaagcaca catacagcac agaacatagt agtagagctt
     5101 gaaaatagag gttataaaaa gaaagatata gagttaatct ctagtggtaa tttttatagg
     5161 ctctttaaag atatactaaa ataatataat aaagaactta aatttataaa atagtatatt
     5221 tatacaaata atatctacat atttaaattg gcgttcataa tttattaaat taaattttaa
     5281 tgttactaga agtttcaatt ctagtaacat tttttatgtt aaaaacagat atagatatat
     5341 attgacaatg ataattatta ttgataaact ataaaagtga aaatgataat aattttcatt
     5401 ttaattaata aaagattttt aaggagggag aatgaagaaa aggtatttag tcataggaat
     5461 aataattttg tcttattttt ctttatttat aggagctgaa gatataaata taatgcatat
     5521 atttgccaaa aatcagcata aacttatgat atttatcatg agtagaatac caagattaat
     5581 aagtatattg attgctggtg taggaatgag catagcaggt cttataatgc aacagataag
     5641 taagaataaa tttgtatcac ctacaacagg tgctacaata gatgcagcac aatttgggat
     5701 agtaatatgt atgcttttag taccaactgc ctctattttt acaaagacaa taatagcatt
     5761 tgtattttct cttgtaggga cttttacgtt tatgaaaata atagggaagt tacaatttaa
     5821 aaatataatc tttgtaccat tagtaggtat aatgtttgga aatataatag gttctatgac
     5881 ggattttatc gcgtataaat acgatttgag tcaaaatgtt agttcttgga tgcaaggtga
     5941 cttttctatg atacttaaag gaaattatga gatattatat atcacaatac cccttataat
     6001 attagcatat atttatgcaa ataaatttac tgttgttggt atgggtatgg attttgcgac
     6061 aaacttaggt cttagttaca aaagaattgt caatataggt ctgataatag tagcacttgt
     6121 tacagtatgt gtagtcgtta cagctggtaa tatccctttt ataggtctaa tagttcccaa
     6181 tatcgtatcc ttgtatatgg gtgataatat aagagcaagc atatggtata ctggtttact
     6241 tggagcgata tttgtactta tatgtgatat ttttggaaga attattattt atccatatga
     6301 aatatctata gggcttacag ttggagttat aggaagtata ctattccttt atttaatatt
     6361 aaggaggaat gtaaatgaag cttagtctca atcaaaaaat ttactttgta agtatattac
     6421 tattcatatc aatagcactt tttataattt atggaataga catgaatcat ttagaatatg
     6481 cattatcaca aagaatacca aaaatatttg ctatggtatt aggtggagga tgtatagcat
     6541 ttactactgt agtgtttcaa acaattacaa acaatcaaat attaacacca agtgtactag
     6601 gtttagattc attgtatgta atgattcaaa ctataatagt attcatattt ggttcaagca
     6661 gtagtctgat tataaatgaa aattataatt ttattattaa tgtagctatg atgattggag
     6721 cttcattgtt attatataag acgttatttg aaaaaaataa aaataatatt ttctttttaa
     6781 tcctagtagg aatgatattt ggaaccttat ttaaaagtgc aacaacattt attcaggtaa
     6841 tgatagaccc taatgaattt ttagctttac aaactagtat aatggcaagt ttaaacaata
     6901 taaacactaa tgtattgtta atagcattta taataattat tgccataata ccttttatat
     6961 atgatgaaat aaagtattta gatgtattgt cgttaggtaa agaacaagca attaatttag
     7021 gtgtagattt tgataaagca gttaaaaaaa tgataatatt aatagcaatt ttagtctcaa
     7081 tatcaacagc acttattgga cctatgacat ttttaggact tttacttgct aatataacaa
     7141 gagaaatttt taaaacatat aagcatacat acttgatttc gggttctatg ttgataggga
     7201 tgattacatt gataataggt caatttttta tacaacatgt atttaagttt gatacaactt
     7261 taagtgtagt aataaatttt ataggaggaa tttactttat tcaattacta ttaaaggggg
     7321 caaatagatg atagagataa aaaatatatt taaaagatat aagaataaaa atgttgtaga
     7381 tgatgtctct tttagtatag aaaagggaaa gataacatct tttataggac caaatggagc
     7441 tggaaaaagt acagtactat caatagttac aagacttata ggaggtgatg gtggagaagt
     7501 aataatagaa ggaaaaagct tgactaatta tagcaataaa gaattagcaa aaaaaatagc
     7561 tattcttaaa caatctaaca atataacttt aaagcttaca ataagagaat tagttggatt
     7621 tggaagattt ccatatagtg aaggtaatct aactaaagag gatgaaaatt atattgatga
     7681 agcaatagaa tatatgaagt tgactgatat acaacataag tatttagatg aattgagtgg
     7741 aggtcaaaga caaagagctt atatagcaat ggtaatagct caagatacag agtacatatt
     7801 acttgatgaa ccactgaaca atttggatat gaaccattcc gtacagatga tgaaggtact
     7861 tagaagtctt tgtgatgagt tagataagac tatagtatta gtaatgcatg atataaattt
     7921 tgcatcatgt tattcagata atatagttgc attaaaaaat ggaaaagtta aaaaagtagg
     7981 tcgtacagat gagatagtaa atgaaaaagt tttagaagat atttatgaga tgaattttaa
     8041 cattaagaat ataaatggaa atcgaatctg tatttatttt taaaatattg tatatgaaaa
     8101 taaacttgct atatgatttt ttataaagtt tttaaattaa aataaagatt aataggagag
     8161 aggaaaatat tgatatgaat aaaaaagcag caatagtagc agcagtagct attataggat
     8221 tagtgacagt gtttgcatta ggtggaagta aaaagaatga atcaaaaaca tcaggaaatt
     8281 caaataatac catgaagata actcataact tgggagaaac agatgtaaaa ttaaatccta
     8341 aaaaagtagt ggtatttgat tattcggctt tagatacaat ggatgcatta ggtgtagctg
     8401 agaatttagt aggacttcca aaagcgagtt taccagcatc cttagaaaaa tataaggatg
     8461 gcaagtatgc agatttagga ggattaaaag aaccagattt agaaggtata aagtcggcaa
     8521 atccagattt aattataata aatggaagac aagaagattt ttatgagcaa ttgtcaaaaa
     8581 tagcaccaac aataagtaca agtaaagatg ataagaagta cttagaatct gttaaaaata
     8641 atatagacaa aatagcaaaa atatttggag tagaagaaaa agcaaatcaa gaatttagta
     8701 aaattgaaaa gaaaatagaa actttgaata aaaaagtaac agataaaaat ttaaatgctt
     8761 taactataat ggtaaatgaa ggtaatttaa gtgtatttgg tgaagaatca agatttagta
     8821 tattatacaa tagttttgga tttgaaaata aagataaaaa tataaaagaa tcaagtcatg
     8881 gacaaaatat tacttttgaa tatatagcta aacaaaatcc agaagttatg ttcgttatag
     8941 atagaggaat agctactggc agtgatgtaa aagaaagctc aactgcaaaa tctgttttga
     9001 acaatgatat aataaaatct atggatgcat ataagaatga taatataata tatttagatt
     9061 caccaacatg gtatgtaaat gatggtggtc taacatcttt aaataaaatg atagatgatg
     9121 cttcaaaagc ggtaaattaa taatccgata taaccaaaat gtatttttta taaaatgatt
     9181 atcaaataaa ctaggagttt ttaagaactc ctagtttatt ttttgcataa aaaagatgta
     9241 gtttaaacaa taatattatc tataataaga taaatctatt caaaatctaa taaaatatga
     9301 agttatact
//
LOCUS       DAMJYC010000132         9307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJYC010000132 DAMJYC010000000
VERSION     DAMJYC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.138"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            330..1346
                     /gene="gap"
                     /locus_tag="PC472_003538"
     CDS             330..1346
                     /gene="gap"
                     /locus_tag="PC472_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2728835.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1741..2910
                     /locus_tag="PC472_003539"
     CDS             1741..2910
                     /locus_tag="PC472_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728836.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3290..7543
                     /locus_tag="PC472_003540"
     CDS             3290..7543
                     /locus_tag="PC472_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2728837.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7692..8186
                     /locus_tag="PC472_003541"
     CDS             7692..8186
                     /locus_tag="PC472_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2728838.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8302..8679
                     /locus_tag="PC472_003542"
     CDS             8302..8679
                     /locus_tag="PC472_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2728839.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8885..>9307
                     /locus_tag="PC472_003543"
     CDS             8885..>9307
                     /locus_tag="PC472_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2728840.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDT"
BASE COUNT         3778 a          957 c         1667 g         2905 t
ORIGIN      
        1 aaataaaaat ttatttttat tattttaaat tagaggagat tcttgaagat atattatttt
       61 taaaaataag tatagtttaa aaattcaaaa aaaagatggc aaaatatgtc ccaagggaca
      121 ttgagtaaaa taaatgattt cagcttattt acactaaaaa gtacatacta aatgaggggt
      181 aaaataaaga aaaatgatat actaattgtc ttttgataat aaaaaaacat ggaatttgat
      241 tatttaatca agaaaaagta ttgaacaaat gatttattta gtgtacaata atcatgtaaa
      301 aacatagtaa tggagggaga catttcataa tgaaaattaa ggtaggaatg aacggatttg
      361 gaagaatagg aagagctgta ttaagaatag ctcaagaaga attaggagat aatatagaaa
      421 ttgtagccat aaatgcaaga gcaacaacag aatctttggc acatttattt acatatgatt
      481 catgctatgg aacttttaga ggtgaagtag aggcaaagga tgaagacact ctagtagtaa
      541 actctaaaga gataaaaata ttaagatata atgacccaga agaattacca tggaaagaac
      601 tgggggttga tatagtaata gaatcaacag gattatttac acaaagagaa aaggcagaaa
      661 agcatattaa agcaggagca aaaaaagtaa ttataacagc tccaggaaaa aatgaagata
      721 taactatagt tatcggagtt aatgagaaag aatatgataa tgaaaagcac aatataattt
      781 caaatgcatc ttgtactaca aattgtctag ctccttttgc taaagtattg gacgaaaaat
      841 ttggtatagt aaaagggtta atgactacag tacattcata tactaacgat caaagaatat
      901 tagataaaag ccataaagat ttgagaagag caagagcagc ggctgaatct ataataccta
      961 ccactacagg agcagcaaaa gctgtgtcaa gagtactacc acaattagaa ggaaaattaa
     1021 atggtttttc acttagagta ccaacaccaa cagtttcact tgttgattta gtgtgcgaac
     1081 ttaaagaaaa cgttacatct gagcaagtta actctgtgtt aaaagatgct gctgaaggag
     1141 aattaaaagg tgttttagga tattgtgaca agccacttgt ttctatagat tataaaggag
     1201 attcaagatc atctattata gacgctctat ccacaatggt tatagaagac aatatggtta
     1261 aggtagtttc ttggtatgat aatgaatggg gatattcttc aagaactgtt gacttagtaa
     1321 aatatgtagc tgaaaaatta aaataatata tatttcttaa agcaaatttt aaaaaaattt
     1381 tttaaaaaaa ttatataaaa ctaaaaaaaa agattatgaa aaatattgaa ggaaaaattt
     1441 tttcataatc ttttttcttt catgcaaaaa gatacaagtt acaacttata tttgcatatt
     1501 aggtcgttag agaagtatat atatatttag taaggaatca tactttttat aaaatgaaag
     1561 ataagatgtc gaaaacttgc ataaatctga aaaattttta ttattaaaat actgatttcc
     1621 attttaaaaa tgataaaaaa atgacaaata aagttgcaaa tagtaatact tataagtata
     1681 attaaaaaag agaaaaggtt ttgttaaact ttataacaaa aaaagaaggg atgattcaaa
     1741 atggagaagt taacagacag gagacaggtt aaggcagtaa gcatggaaac cttcattttc
     1801 ctaatattac tagtagttgg ttttgggtat gttggtagta ttatgggagc aggaatgatg
     1861 ttcaaagtaa taatgagtac agcacatgct ttactattgg agacagtttt cttaattatg
     1921 gctatggcag ttcttgcagg cgcattaagc gcactacttt cagagtttgg agtaatagcg
     1981 ttaattaata aaattttcgc tgtatttatg aagcctttat atggactacc tggtgctagt
     2041 atagcaggag ctataacaac ttatttatca gataatccag ctattatacc ttttgctaaa
     2101 gataaaacat tcacacagta ttttaaacaa taccaagttc cagcattatg taacttagga
     2161 acagcctttg gtatgggact tatcttaaca acatttatga tttctcaagg tacagaatat
     2221 gtacttccag cattaattgg taatttaggt gcaattatag gtagtgtcat aagtgttaga
     2281 ataatgttga catttactaa gaagttttat aaatacaatc cggaagaaga taaagcaaca
     2341 ggaacattag agaaaaagga tgaatttaga gaaatcagag aaggaaatgt atttcaaaga
     2401 gctttagatg caattcttga aggtggtaaa atgggggtag atatgggaat ggctataatt
     2461 cctggagtcc tagtagtatg tacattggtg atgttattaa cttttggacc atctacagat
     2521 cctgttacag gtcaagaagt ttatacagga gctgcatatg aaggaataaa attacttcca
     2581 gttattggag ataaacttgg atttatatta gaaccattat ttggatttac atcaccagag
     2641 gcaatagcat tccctataac ttcattggga gctgttggcg cagcaatgtc tctagttcca
     2701 gagtttataa agtcaggagc tataactcct aatgatatag cagtatttac agctatgggt
     2761 atgtgttgga gtggatattt aagtacacat attggtatga tggatgcatt aaatgctaga
     2821 cagttggcag gtaaagctat tctttctcat acaataggtg gtttatgtgc aggagcagct
     2881 gctcacttta tatttacact cgtaggataa atgtaatcag ctaagataaa aaataatata
     2941 caaaaaatgg ttgttgatat gtaataagaa tttacatatc aacaaccatt tttttctgac
     3001 taaagataat ttataataca taaacattaa gtggatatta gagttaagtt atatttatgt
     3061 aaaaagtaac aaaaatctgt agaaatttat gacaaaattt agtacactaa ctatatgttc
     3121 ttatttaaaa tttaaggtta gaattaggaa ttacctagta gagtaagctt tgattaataa
     3181 aagaatcata aaataaaaat attagtcata aagattataa ataacttact taaaataaag
     3241 ccatagaact tacaaaaatt aaaaaagcat ataaatatgg aggggtacaa tggatgaatt
     3301 atttcatata ggaattgatg taggttctac aacggtgaag gttgtggtac ttaataatag
     3361 taataatata gttcataaag aatatagaag acattattct gatgtaaaaa aatcagttaa
     3421 agaagtacta aatggtatat atgaaaaatt gggagatata aatactacca ttataataac
     3481 tggttcaggt ggaattggaa tatcaaaaaa attaggtgta aaatttgttc aggaagttat
     3541 atctagtaca aaatcaatag agtattttca tcctgaaact gatgttgtca tagaacttgg
     3601 gggtgaagat gcaaagataa catatctaag tggaggaata gaccaaagaa tgaatggaat
     3661 atgtgcaggt ggcactggag cattcataga ccagatggca tcacttttaa agacagatgc
     3721 cagtggatta aatgaacttg ctaaagggta taatgttata tatcccatag cttcaagatg
     3781 tggtgttttt gctaaaacag atattcaacc tcttataaat gatggagcca atcagactga
     3841 tatagcaatg agtattttca atgcagttgt cgtacaaact gtgagtgtcc tttcttgtgg
     3901 aagaaaaata gaaggaaatg ttgcgttttt gggtggtcca ttgtactttt tatctgaact
     3961 tagagaagca tttaaaagag tattagattt aaaagatgaa aacataatct tcccagaaaa
     4021 tgcacaactt tatattgcta ttggagcagg attattatca gtagatgaag ataatataca
     4081 actaaaatct ctaatagaaa aattggatag tataaagaat attgaagatg gagaagtaaa
     4141 tttattagaa cctttattta aagataaaaa tgagtatgaa gaattttcaa gaagacatga
     4201 aaaagaaaaa ataaattatg tggacataaa ttcaataaaa agcaattgtt atttaggaat
     4261 tgatgcaggc tcaactacaa ctaaagcagc acttattgat gaggatggaa gacttgtata
     4321 ttcttattat aatagcaatg aaggaaatcc attaaaaaca accattaaag ttataaatga
     4381 agtatatgat atattaccaa aaaatattaa aatattgagt tcaactgtaa ctggatatgg
     4441 agaaggtctt attaaaaaag ctcttaaaat cgataatgga gagatagaga ctatagcaca
     4501 ttacaaagct gctaaatttt tcaacaaaga tgtagatttt atacttgata ttggtggtca
     4561 agatatgaag tgcctaaaaa taaaagatgg agtaattgat aatataatat taaatgaggc
     4621 ttgttcatca ggttgtggct catttttaga gacatttgca agttctttat ctatgacaat
     4681 agaagaattt gcgcatgaag gtatttattc tcaaaatcct gtagatttag gttcaagatg
     4741 tactgtattt atgaattcta gagtaaaaca agctcaaaaa gaaggtgcaa gtgttggaga
     4801 tatatcagca gggctatctt attcagtaat aaaaaatgct ttatttaaag taataaagat
     4861 tagagattta aatgagattg gtaataatat tgttgtacaa ggtggaacct tttataatga
     4921 tttggtgctt agaagctttg aaaaactaat tggaaaaaat gtaattagac ctaatatttc
     4981 aggaattatg ggagcttttg gttcagcact tatagctaaa gaaaaatatg aaagtggata
     5041 caaaacatcc ttgttatcta aggaagagtt gaataatatc aagctagaag caagtgtaac
     5101 tagatgtaaa ggttgttcaa atcattgtct tcttacaata aataaatttt ctgataatga
     5161 aatttttatt tcaggtaata gatgtgagaa gggtgaagct atttatggtg ataaaaaaat
     5221 aggattagaa aagaaaaaac caataaattt atttaagtac aaatacaata gaatttttag
     5281 atacaaacca ttggaaaaag aagaagcaaa aaatggggaa atagggatac caagagtatt
     5341 aaatatgtat gaagattatc cattttggtt tacttttttc aatgctatgg gctttagagt
     5401 tgtactttca gataggtcat caaaacagtt atatgaaact ggtataacta gtattgcatc
     5461 tgaaacagtt tgttatccag gaaaattagt tcatggacat atagaaaatt taattgcaaa
     5521 aggtataaaa aatatatttt atccaagtgt aacaaatgaa aataaagagg attcaaatgc
     5581 agataattat tataattgtc ctgttgtaat atcatattct gaagtaataa aaaacaatgt
     5641 agagaatata agaaataaga acataaatta tataaatcca tttataagtc taaatgataa
     5701 ggaaaaattg aagaaaagac tctatgatga attatcaaga catttttctg atataaaaat
     5761 aactaaagaa gaagttaatc atgcagttga taaagctact gatgaacaga atttatttaa
     5821 aatggagata caagcagcag gtgaaagagc acttaaagaa ataaaagaaa gaaatatgaa
     5881 ggggattgtg ttatgtggaa gaccatatca tatagaccca gaaataaatc atggaatgcc
     5941 agagcttata aattcattag atatggcagt gcttactgaa gactctatat gtcatttagc
     6001 caatgtacaa agaccactta gagttgtaga ccaatgggta tatcattcaa gattgtataa
     6061 agcagctagt tttgtaagag ataaaaacta tttggaactt gttcaactta attcatttgg
     6121 ctgtggatta gatgcagtaa ctacagacca ggttcaagaa atacttaatg aaaaatcgaa
     6181 gatatatact attataaaga ttgacgaagg caacaattta ggagcagcta agataaggat
     6241 gaggtcctta aaagcagcta tgtttgagag agaaagcaaa aatatagatg taaaaaatat
     6301 aaaggtaaac aggatacagt atgctaaaaa taatcagata acgaacaaac atactatatt
     6361 agcaccacaa atgtctccaa tacatttcca attccttgaa aaagctgtta acttaagtgg
     6421 ttataatata gaaattctaa aagatactga tagtagtgta attgaagaag ggttaagata
     6481 tgtaaataat gatgcatgtt atccagctat aatagttgta ggtcagctaa taacagcatt
     6541 aaaatctggc aaatatgatt taaataacac ttctgttact ataactcaga ctggtggagg
     6601 ttgtagagct acaaattata ttggattctt aagaaaggct atatacgatg ctggatttaa
     6661 agatgttcct gtcattgcac ttagtgtaaa tggaatagaa gatagtggta taatggataa
     6721 tatatcacta aaattaatca atagactctt tatgtctgtt gtttatggtg atttgctgat
     6781 gaaagtacta tatagagtta gaccatatga aaaagttact ggaagtgcaa atgctctata
     6841 tgaaaaatgg gttgatatat gcaaatcttc tcttgttaaa gcaaaaattt ctgtatttac
     6901 tagaaatata aaagatatag taaaagaatt tgataattta gagatactag atatcaaaaa
     6961 accaaaagta ggtttggttg gagaaatatt ggtgaaattc catccaattg caaataataa
     7021 tttagtagat atattagagc gagaaggtgc tgaagcagtc gtgccagacc ttacaaattt
     7081 cttcttaagt tgtgccttta atactatata caaacacact catttagaag gaagtagaaa
     7141 gagtagaatg ataggagagg catttatata cataactgga atatatcaaa gagtatataa
     7201 aaaagcactt gataagagtg agagatttta tgctccagcc aatataaaaa atgttgctaa
     7261 aagtacagaa ccagtagtgt cattaggtaa tcaaactgga gaaggttggc ttttaacagg
     7321 tgaaatggtt gaattattaa atgaaggtgt agaaaacatt atttgtatgc aaccttttgg
     7381 atgtttacct aatcacataa ttggtaaggg ttctataaaa gaattaaaga ggttgtataa
     7441 aaatgcaaat ataataccta ttgattatga cccttctgca agtgaggtaa atcaacttaa
     7501 caggataaaa cttatgcttt ctaaggcttt taaaaatata taagatttat attttgatat
     7561 gttaaaagtt aatgaattta aaaatatact ctatattaag tatctataaa aaagagttat
     7621 cctatttttt agggtaactc tttattagtt aaaaaattat atgtatactg atattaaagg
     7681 ggggaagtaa tatgagttta gtatatgatg tttataaatc aaaattagga aatttgtaca
     7741 tattaagtga agatggtttt atagttagta tatacatagg agatgaaaaa tttaataatt
     7801 taaaaaatga atgtattaaa aaaggaattg atataaagag aagtaaggaa gatttaaagg
     7861 aagcaataag ccagatagat gattacttta atggaaaaag aaaagtcttt aatttaaata
     7921 ttcgtattga agggactgaa tttcaaaaat cagtttatag ggaaatgtta aaaatacctt
     7981 atggagaaac attgtgttat tcagatatag caagaaatat aaacaatcca aaatcagtaa
     8041 gagcaatagg acaagcaagt aaaagtaata aaattccaat aataatacct tgtcatagag
     8101 tagttggcaa aaaagatatt ggtggatata tggggaatca ttctgatttg aaagagatac
     8161 tattaaattt agaaagacag agttaactta gaaagaaatg tttttattgt taatatttga
     8221 tataataata gtaaaaagaa gtatttttta caaaaacaat gaagaattta ttttaattta
     8281 caaaaatgag ggggaatttt aatgtgtgat aaagttatag catcaaaata tcatgagaag
     8341 ggatttaatt gtgcagaatc agttataaaa gcatataatg aggaatttaa tacagatata
     8401 ccagtttgtt taggaagtgg actgggttca gggtgtgggg ttgctagttt atgtggtgca
     8461 gtaaatgcat caaatataat aattggatat gtaaaaggca gaaatcacga agatgaaagt
     8521 actaaagcta aagtatatgc taaagattta actacaacag taagaaaaga gtatggttca
     8581 gagttatgta tagacctaaa aaaagattta gttgcttgta gagaaataat ggattttgca
     8641 tatgatagtc taaaggaaac tttaaaaaaa gaattataag aaaataagct atcaagtata
     8701 aattatttga tagcttattt ttatagtcga tactaaataa atcaaatata ttttagaatt
     8761 attatttgta aataaacagc aaataatttt aggattttta tttaatatag tttttattta
     8821 tatttatttt tgaatatgtt atactttgtt taaataaata tttgtaatga gggagatgag
     8881 aagattgaag aatctcaatt tacttattaa taaattatat tcaaaaaatc ataatgaagc
     8941 atataaaact tttttattct tagaaaatga aagtttaaaa tcaaatatta catattgttt
     9001 ttttgactca ttcctagaaa tgattgataa cgaaaactca tatattcgaa caaggggatt
     9061 gttattaatc tctgcaaatg ctcagtggga tattgataat aaaattgaaa taaatataga
     9121 tagtatacta agtcatatcg tagataaaaa accttctgta tcaagaatgt ttattaaatc
     9181 catacctaat attacaaagt ataaagaaaa tcttattaat cgcataaaaa tggaattaag
     9241 taatgcagat ataagtattt ataataataa tatgaaacca ttagtagaaa aggatataaa
     9301 cgataca
//
LOCUS       DAMJYC010000133         9148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJYC010000133 DAMJYC010000000
VERSION     DAMJYC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.139"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..518
                     /locus_tag="PC472_003544"
     CDS             <1..518
                     /locus_tag="PC472_003544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728841.1"
                     /translation="TPSNPDTGKPQSKPEEKKPEAESKKEERKKYDESKKAEEKKSDV
                     SKTETLSNPDTGKPQVKLEEKKPEVDVKQNEEKPDAKHYNKNTEKELNKDELNKSKPN
                     EISMVKKENNKNVIKDQSKVMSSRESKAETKNIKDVPKTGEASSLPYIGGILISLAAF
                     LKLNMKKFKKN"
     gene            809..1204
                     /locus_tag="PC472_003545"
     CDS             809..1204
                     /locus_tag="PC472_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2728842.1"
                     /translation="MNKKVIFSIGIVSLGVALSGCSEKGMDIKDNKSERVSITANSAT
                     FKDGTFEGEGEGFENGKTKVSVEVQDGKIKKIDVLNSEDDESYLDEAKKLIPEIIKKQ
                     SSDVDSISGATYSSKGIISAVENALKNAK"
     gene            1217..5320
                     /locus_tag="PC472_003546"
     CDS             1217..5320
                     /locus_tag="PC472_003546"
                     /inference="COORDINATES: protein motif:HMM:NF016121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2728843.1"
                     /translation="MRKKSKIIAGGILITSVIGATSFAIIDPYNILSNKNMKIPEQSR
                     KLEKSNNKEKEDIDNKKDENNMNLNGIKDGTYLGEAKGYGGNIKVKVIIESGKIKNIE
                     VLSHSETPKYYENGSKVIGNIIRANSTDVDAVSGATLTSNGIKNAVRDAISKAGFNVS
                     KDNKEVSVASNSAKSRPVSSNNMVKNIDLKEYKIKDGEYIGEAIGFKGNVKVKVIISG
                     GKLSDVKVISHNDDAEFFNKAKSVIIKILRNQGTAGVDTVSGATYSSRGIINAVNSAL
                     NKVAKESNGIFNTIKIFENDNIPRKDSNPKFNVKDIVKEALHQKSISIDKDDNISSRE
                     HKFKDGEYIGEANGFKGNVKVKVIIKNGTLVNIEIINHNDDKEFFNNAKRLIFKILKN
                     QGTTGVDTVSGATYSSKGIINSVNRALNKAVNKNITTQDTIVQSKGDKVKENVDIKDI
                     ENPNDKFTQKENQEISKDNKEKESSKSVKYLDGSYKVSGIGFTGKPIKSVVTFENNKI
                     KSIDIGTIESGDFGDNEPFRPIAIKVVDHILSDNGGKSINDLILHGEIVDKIFKSNNY
                     YEVGKNLIGDYAGELKGIYVSGRIAHEKISSVVKKYMKAKNSSTVLDSVSGATFSAIG
                     IAKSVKDAMNKSANDYETDNIVNELKIKTPSEKRIYVDLVYSTKDKKLDLSNLKVIIS
                     MRDGKEKEISYDKFKENDIEIHDKETGKAITNGMDLSSYESGHGIYATIKHKKSLSTD
                     TLLIIPRFFDKMDTNHLTRMEYSIDNGTSWKALSSLEMNEKNINFYQTIKLPKEFKGK
                     DISLRVVSTNKNTYLLKPEKSGQSLKVEKEKYDLITNEEDRLSNHNLRDRYRIVFEFI
                     ENSGNVNKEIELNNKDIKYLDGKYIGIGKGWTSDPIKSKVIFENNKIKNITIEDFPDD
                     IECRDKAEKVIPLLKENPENIINDMLLLDEIAGKIVSSDNYYEKGKELIGNYAENLNG
                     FEKNTFVTVAKYLREQKKAAVFDVVSGATYSAKGIVRSVKDAMDKSANDYKTGNIINN
                     LKIKSPSNKNMRVDKKEKLDLSNLKIVISMRDGKEKEIGYNELKENGIEIYEEETKKT
                     IYNGMDLSSYERNHAICAVIEHKNSLNKDKLIIIPEIIDKNYITGMEYSVDNGVTWKN
                     ISGLKKMNNSNNIHFNQELKISKEDERKVLLRAVSINGDKYGLNLKEKIEGPNGYCIF
                     EPSKDDKAKNKHVANGFNISFKFTNTTDNNVATSLEKVSYARIYLNDSVIYKQYQGKL
                     KVGQKIDWTGAKFKFKNSEQESIKDLFTEDEIKNLKNSNSYKKEGYFNYEDLEKLGIT
                     ISPNQNSEVTTDLNVTLTYKGKTFKVKGGGINGDDDFVGIILEKQEK"
     gene            5660..6979
                     /locus_tag="PC472_003547"
     CDS             5660..6979
                     /locus_tag="PC472_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2728844.1"
                     /translation="MKRVYQHINVYEALQERLKFIFQEFENIFVSFSGGKDSALLLHL
                     VIDYKRRNNLDTKIGVFHQDFEAQYQITTEFVEEIFEKYILDIEPYWVCLPMAVRTAL
                     SNYQLYWYPWDDKNKEIWVRDLPEKPYVIHMGNNPFYLYKYRMLQENLAKQFGRWYKQ
                     VHGNKKTVCLLGIRADESLQRYSGFLNNKNGYKGECWLSKKFKDVWTASPLYDWKITD
                     VWTAYYKFGYTYNKLYDLYYKAGLKPSQMRVASPFNDYAKESLNLYRILEPKTWTKLV
                     GRVQGVNFTAIYGKTKAIGYRNITLPEGYTWQEYTKFLLATLPARVRNSYIKKFNTSI
                     KFWHKTGGGLSEETINELLENGYKIKRNGVSNYTLDKKSRIVFMQKIPDDTDNIKSTK
                     DIPSWKRMCYCILKNDHLCRFMGFGLKSDEQRKIDTIRKKYKGLEEI"
     gene            6979..7512
                     /locus_tag="PC472_003548"
     CDS             6979..7512
                     /locus_tag="PC472_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2728845.1"
                     /translation="MDFKSPVYNIVAIPVEKIVPNTYNPNAVAPPEMKLLYDSIKEDG
                     YTQPIVCYYSQKKDKYIIVDGFHRYRIMIDYLDIYEREKGKIPVVIIDKPLDCRMAST
                     IRHNRARGSHDVDLMSNIIRELHEIGRSDAWISKHLGMDKDEILRLKQITGLTYLFRD
                     VEFGKAWIPEETIYNEE"
     gene            complement(7651..9099)
                     /locus_tag="PC472_003549"
     CDS             complement(7651..9099)
                     /locus_tag="PC472_003549"
                     /inference="COORDINATES: protein motif:HMM:NF016901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728846.1"
                     /translation="MFKENLLESSEKIEIEILKKLYINNGIFSKYKLCDELHISFPTL
                     KLYIRNINMMFFNYYNDDVNIHINKETIFLKYRNNISLDNFVSIYIENSLKYKLLSML
                     YYHKNLNSVKLCSYLNISLSTLNRKINECNKLLNSFELRIKKFDLKGSPLQFAYFYYS
                     LFRNSGVNKEITPGLNDKDIVKFLEDSMSIDFTYAQKLSIYIWSKIIIKNRDKFTKEN
                     FSDKFSLSNLDSFKNDITFKNLELFYSKFPHYKKYLAYATACFLKTFGVLAFENTVDI
                     TLPFNVHNFILSRMKSLFLNKDFEFDKTVKSNILSYCNKQFYFKGIFYSIDENTRDFY
                     LNSHLSFLKDYFIVDLFKDIKLSFKLDNLDFDYFKLLIVLNLSYIDEESKYTIKIGVL
                     SKTENLAFKVLLKDFNNLLSKKFNAKAEVLSEKNLHTYDLIISNIHDQFSDGMSTTIF
                     KFTYLGVEYDLNNLTEILNNIEKEKIENLRLG"
BASE COUNT         3893 a          876 c         1533 g         2846 t
ORIGIN      
        1 aaactccgag caatccggat acaggaaaac cacaatctaa acctgaagag aaaaagccag
       61 aagcagaaag taaaaaagaa gaacgcaaaa agtatgatga aagtaaaaaa gcggaagaaa
      121 agaaatcaga tgtaagtaaa actgaaactt taagcaatcc ggatacagga aaaccacaag
      181 ttaaacttga agagaaaaag cctgaagtag atgttaaaca gaatgaagag aaaccagacg
      241 caaaacatta taataaaaat actgaaaaag aattgaataa agatgaatta aataaaagta
      301 aaccgaatga aatatcaatg gtaaaaaaag aaaacaataa aaatgtgata aaagatcaaa
      361 gtaaagttat gagtagtaga gaaagcaaag ctgaaactaa aaatattaaa gatgtaccaa
      421 aaacagggga agctagtagc ttgccttata taggtggaat tttaatttct ttagcagctt
      481 tcctaaagtt aaatatgaaa aagtttaaga aaaactagtt attttattaa ataagatggt
      541 ttttaataaa tttaaatgta tgaatcaatt aacatattga taatgagaat caatattgta
      601 taactagaaa atgcatattt aaatattaat aaaaataact attatcttaa ataataaata
      661 tcactaccat attgaaactt ttcaaaatgg tagtgatatt taaagtataa gcgttattaa
      721 ttttaattta tttacattaa gaataaatct tatattaaaa agtagtaaat ttgatataag
      781 atactaaaac tttttaagga gtattgagat gaataaaaaa gttatttttt cgataggaat
      841 tgtttcctta ggagtagcac tttctgggtg ttcagaaaaa ggtatggata taaaagataa
      901 taaaagtgaa agggttagta ttactgctaa ttcagctacc tttaaagacg gtacttttga
      961 gggggaagga gaaggatttg aaaatggcaa aacaaaggta tcagttgaag tacaggatgg
     1021 aaagattaaa aaaatagatg ttctaaattc agaggatgat gaaagttatt tagatgaagc
     1081 taaaaagttg atacctgaaa taataaagaa gcaaagttca gatgtagact ctataagtgg
     1141 ggcaacttat agttcaaagg gtataatttc agctgtggaa aatgcattga aaaatgctaa
     1201 ataggaggaa gaacttatga gaaaaaagtc aaaaataatt gctggtggta ttcttattac
     1261 aagtgtaatt ggtgcaacga gttttgcaat aatagatcct tataatatac ttagtaataa
     1321 aaatatgaag attccagagc aaagtagaaa acttgaaaag agcaacaata aggaaaaaga
     1381 agatattgat aataaaaaag atgaaaacaa tatgaattta aatgggataa aagatggaac
     1441 gtatttagga gaagcaaagg ggtatggagg aaatattaag gtaaaagtta ttatagagag
     1501 cggaaaaatt aaaaatattg aagtgctttc tcattcagaa acaccgaaat attatgagaa
     1561 tggaagtaaa gttattggaa atattataag agcaaattct actgatgtag atgctgtttc
     1621 aggagcaact ttaacttcta atgggataaa aaatgctgtt agagatgcaa tatctaaagc
     1681 tgggtttaat gtaagtaaag ataataaaga agtaagtgta gctagtaata gtgctaaaag
     1741 tagaccggta tcatctaata atatggtcaa aaatatagat ttaaaagaat ataagattaa
     1801 agatggagaa tatatagggg aagctatagg gtttaaaggg aatgtaaaag taaaagtgat
     1861 aataagtggt ggtaaattat cagatgttaa ggttattagc cacaatgatg atgcagagtt
     1921 ttttaataag gctaaaagtg taattattaa aatattaaga aatcaaggga ctgctggagt
     1981 tgatacagtt tcaggagcta cttattcaag tagaggaata ataaatgctg ttaattcagc
     2041 tttaaataaa gtagctaaag aaagtaatgg aatttttaat acaataaaga tttttgaaaa
     2101 tgataatata cctagaaaag atagcaatcc aaagtttaac gtaaaagata ttgttaaaga
     2161 ggcattacat caaaaaagta tcagcataga taaagatgat aatataagtt caagagaaca
     2221 taaatttaaa gatggagaat acataggaga ggctaatggc tttaaaggta atgtaaaggt
     2281 aaaagttata ataaaaaatg gtacattagt aaacattgaa ataattaatc ataatgatga
     2341 taaggaattt tttaacaatg ctaagagatt aatatttaaa atattaaaaa atcaaggaac
     2401 tactggagtt gatacggttt caggagctac ttactcaagc aaaggaataa taaattcagt
     2461 caatagagct ttaaataaag cagttaataa aaatattact actcaagata caattgtaca
     2521 gtctaaaggt gataaagtaa aagaaaatgt agatattaaa gatattgaaa atccaaacga
     2581 taagtttact caaaaagaaa atcaagagat atctaaagat aataaagaga aagaatcaag
     2641 caagagtgtt aagtatttag acggaagcta caaagtatca ggaataggat tcacaggaaa
     2701 gcctattaag tctgtagtaa catttgaaaa taataaaata aaaagtatag atattggaac
     2761 aatagagtcc ggagattttg gagataatga accttttaga cctatcgcta taaaagttgt
     2821 tgatcatata ttaagtgata atggagggaa atcaattaat gatctgattt tacatggaga
     2881 aatagtagat aaaatattta aatcaaataa ctattatgaa gtaggaaaaa atttaatagg
     2941 tgattatgca ggagagttaa agggaatata tgtaagtggt cgtattgcac atgaaaaaat
     3001 atcatcagtt gtaaaaaaat atatgaaagc taaaaatagt tctactgttt tagattcagt
     3061 ttcaggagca acttttagtg ctataggaat tgcaaaatca gtaaaagatg ctatgaataa
     3121 atcagccaat gactatgaaa cagacaatat tgttaatgaa ttaaagataa agacacctag
     3181 tgaaaaaaga atatatgtag atttggtgta ttcaactaaa gataaaaagt tagatttgtc
     3241 caacttaaaa gtaataatat caatgcgtga tggaaaagaa aaagaaatat catatgataa
     3301 atttaaagaa aatgatatag aaattcatga taaggaaaca ggaaaagcta ttacaaatgg
     3361 aatggattta agtagttatg aatcgggtca tgggatatat gctactatta aacataaaaa
     3421 atcactatcc acagatacac tattaataat tccgagattt tttgataaaa tggacacaaa
     3481 tcatttaact agaatggagt atagtataga taacgggact tcatggaagg ctttatctag
     3541 tttagaaatg aatgaaaaaa atatcaactt ctatcaaaca ataaagttac ctaaagaatt
     3601 taaaggtaaa gatatctcac ttagagtagt atcaactaat aaaaatacat atttgttaaa
     3661 gccagaaaag tctggacaat ctttaaaggt agaaaaggaa aaatatgatt taataacaaa
     3721 tgaagaagat agattaagca accataatct tcgagatcgt tatagaatag tttttgaatt
     3781 tattgaaaac tctggtaacg ttaataaaga aatcgaatta aataataaag acattaagta
     3841 tttggatgga aaatatattg ggataggaaa aggatggaca agtgatccaa ttaagtcaaa
     3901 agtaatattt gaaaataata aaataaaaaa tataactatt gaagattttc cagatgatat
     3961 agagtgtaga gataaagccg aaaaagttat tcctctttta aaagaaaacc ctgaaaatat
     4021 tattaatgat atgttacttc ttgatgaaat agctggtaaa atagtaagtt cggataatta
     4081 ttatgaaaaa gggaaagagt taataggtaa ttatgcagaa aatcttaatg gatttgaaaa
     4141 aaataccttt gtaactgtgg caaaatattt aagggaacaa aaaaaagcag ctgtttttga
     4201 tgtggtttca ggagctacat atagtgctaa aggaatcgta aggtctgtaa aagatgctat
     4261 ggataagtca gctaatgatt acaaaacagg aaatattata aataatttaa agataaaatc
     4321 tccaagtaat aagaatatgc gtgtagataa aaaagaaaaa ttagatttat caaatttaaa
     4381 aatagtaata tcaatgcgtg atggaaaaga aaaagaaata ggatataatg aattaaaaga
     4441 aaatggtata gaaatttatg aggaagaaac aaaaaaaact atatacaatg gaatggattt
     4501 aagtagttat gaaagaaatc atgcaatatg tgctgtgatt gagcataaga attctttgaa
     4561 caaagataag ttgataataa ttccagaaat tattgataaa aattatataa ctggaatgga
     4621 atatagcgtt gataatggtg ttacttggaa gaatatttct ggtttaaaaa agatgaataa
     4681 tagtaataat attcatttta atcaagaatt gaaaatttct aaagaagatg aaaggaaagt
     4741 attgcttaga gcagtatcaa tcaatggaga taaatatgga ttaaatttaa aagaaaaaat
     4801 agagggtcct aatggctatt gtatttttga gccaagtaag gatgataaag caaagaataa
     4861 acatgttgca aatggattta atataagctt taaatttaca aacactactg ataataatgt
     4921 agctacaagt ttagagaaag taagctatgc tagaatatat ctgaatgatt ctgtaatata
     4981 taaacaatat caaggtaaat taaaagttgg tcaaaaaatt gattggacag gtgctaagtt
     5041 taaatttaaa aattcagaac aagaatcaat aaaagattta tttacggaag atgaaattaa
     5101 aaacttaaaa aatagtaact cctataagaa agaaggatat tttaattatg aagaccttga
     5161 aaaactagga ataacaatat ctccaaatca aaattcagaa gtaacaactg atcttaatgt
     5221 tacattaaca tataaaggta aaacatttaa agttaaaggg ggaggaatta atggagatga
     5281 cgactttgtt ggaataattt tagaaaaaca agaaaaataa aaccctacat gcctgtttgt
     5341 attattttga acggtttaaa aatatttatt taattttaaa gacaactact aatattatag
     5401 tggttgtctt ttgatgttaa tgaaatttac tataaaaatt caaaaaaatt attctattaa
     5461 taaaaagttg aaaaaattaa atgttttaaa gtgatatcat ataatagaaa aaattaattt
     5521 agaagtctaa gggatttaat attatatgaa aaacagatat acttcatcta attattataa
     5581 ttttaacaat tagataaagt taatgtaagt taaattacca gatatacttt ttaatactat
     5641 ataaatagga gtgaacaatg tgaaacgtgt ataccagcat ataaatgttt atgaagcgct
     5701 tcaagaaaga ttaaaattta tttttcaaga gtttgaaaat atttttgttt cttttagtgg
     5761 aggtaaggat agtgctcttt tacttcacct tgtaatagat tataaaagaa gaaataactt
     5821 agatactaag ataggtgtgt ttcatcaaga ttttgaagct cagtatcaaa ttacaacaga
     5881 atttgtagag gagatttttg aaaaatatat tttagatatt gagccttatt gggtatgttt
     5941 gcctatggct gtaagaactg cacttagcaa ttatcagttg tactggtatc cgtgggatga
     6001 taaaaataaa gagatttggg ttagggatct tccagagaaa ccgtatgtta ttcatatggg
     6061 aaacaatcca ttttatttat ataaatatcg tatgttgcag gaaaatttgg ctaagcaatt
     6121 tggaaggtgg tataagcaag ttcatggaaa taaaaaaaca gtatgtcttt taggaattcg
     6181 tgcagatgaa tcacttcaaa gatatagcgg atttttaaat aataaaaatg gatacaaagg
     6241 agaatgttgg ctttctaaaa agtttaaaga tgtttggaca gcttctccct tatatgattg
     6301 gaaaataact gatgtttgga cagcttacta taagtttgga tatacatata ataagctata
     6361 tgacttatac tataaggctg gcttaaaacc aagtcagatg agagtagctt ctccatttaa
     6421 tgattatgca aaagaatcac taaaccttta taggatatta gaaccaaaaa cttggactaa
     6481 acttgtaggg agagtacaag gagttaattt tacagctatt tatgggaaga ctaaggcaat
     6541 tggatataga aatattactc ttccagaagg atatacgtgg caggaataca caaagtttct
     6601 tttagctaca cttccagcaa gggttagaaa tagctatata aaaaagttta atacttctat
     6661 taaattttgg cataaaacag gtggaggatt atcggaagag actattaatg aacttttaga
     6721 aaatggatat aaaatcaaaa gaaatggagt atcaaattat acattagata agaagtctag
     6781 aatagttttt atgcaaaaga ttccagatga tacagataat ataaagagta caaaggatat
     6841 accttcttgg aagagaatgt gttattgtat tctaaaaaat gatcacttat gccgttttat
     6901 ggggtttgga cttaaaagtg atgaacaaag gaaaatagat acaatacgaa aaaagtataa
     6961 aggattggag gagatataat ggattttaaa agtccagttt ataatatagt tgcaatacca
     7021 gttgaaaaaa tagttccaaa tacatataat cctaatgctg tagctccgcc agaaatgaag
     7081 ttgctatatg atagtattaa agaggatgga tacactcagc ctatagtatg ttattattca
     7141 caaaagaagg acaaatacat aattgtggat ggattccata gatataggat tatgatagat
     7201 tatttagata tttatgagag agaaaagggg aaaatacctg ttgttattat agacaaacca
     7261 ttagattgta gaatggcaag tacaatacgc cataatcgtg caagaggttc acatgatgta
     7321 gatttaatga gtaatattat acgagagctt catgagattg gaagaagtga tgcttggata
     7381 tctaaacatt taggtatgga taaggatgaa attttaagat tgaaacaaat aaccggtctt
     7441 acgtacttgt ttcgagatgt agaatttggg aaagcatgga ttccagaaga aactatttat
     7501 aatgaagaat aagtatcgtg gattaagctt tttatattca ataaggaaac tagataagct
     7561 tatttaaatc aatagacaat aaaagaaaga tgtactatct taaattaaaa aagatagtac
     7621 atctttttaa ttataaaaga ggtagatata ttaacccaat cttaaatttt ctattttttc
     7681 tttttctata ttatttaaaa tttcagttaa gttatttaaa tcgtattcga cccctaaata
     7741 agtaaattta aaaatagtag ttgacatacc atctgagaac tggtcatgaa tatttgaaat
     7801 tattaggtca tatgtatgta agtttttttc agaaagaact tcagcttttg cattaaactt
     7861 tttacttaac aaattattga agtctttaag tagaacttta aatgctaaat tttctgtttt
     7921 agataagact cctattttta tggtatattt actttcctca tctatatatg ataagtttaa
     7981 gactattaat aacttgaagt aatcaaaatc taaattatct aatttgaaag ataattttat
     8041 gtctttaaat aaatcaacta taaagtaatc ctttaaaaag cttaaatggc tattcaaata
     8101 gaaatctcta gtattttcat caatagaata aaatatacct ttaaaataaa attgcttatt
     8161 gcaataagac aaaatattgc tcttaacagt cttatcaaat tcaaaatctt tatttaaaaa
     8221 taaagacttc attcttgata aaataaaatt atgtacatta aaggggagtg taatatctac
     8281 tgtattttca aaagccaaaa caccaaaagt ttttaaaaag caagctgttg cataagctaa
     8341 atacttttta taatgtggaa attttgaata aaataattca aggtttttga aagttatatc
     8401 atttttaaaa ctgtctaagt ttgacagaga aaatttatct gaaaaatttt ctttagtaaa
     8461 tttatctctg ttctttataa taatttttga ccaaatatat atacttaatt tttgagcata
     8521 agtaaagtct atagacatag aatcctctaa aaacttgaca atatctttat catttaatcc
     8581 aggtgtaatt tctttattta cgccactatt tctaaataaa gaataataaa aataggcaaa
     8641 ttgaagagga gatcctttta aatcaaattt tttaattctt aattcaaagg aatttaataa
     8701 tttgttgcac tcattaattt ttctattaag agttgataaa cttatattta agtagctaca
     8761 taatttaaca ctgtttaaat ttttatggta ataaagcata gataataatt tatactttaa
     8821 agaattttct atatatatag aaacaaaatt atctaaacta atattattac ggtattttaa
     8881 gaatatagtt tccttattta tatgtatatt aacatcatca ttgtagtagt taaaaaacat
     8941 catatttata tttcttatgt aaagttttaa cgttggaaaa gaaatgtgca attcatcaca
     9001 aagtttatac tttgaaaata taccattatt aatataaagc ttttttaaaa tttcaatttc
     9061 aattttttct gatgattcca ataaattttc tttaaacata gtagtctcct tttctaaaat
     9121 atagattaaa aatatacaag gtaatgtc
//
LOCUS       DAMJYC010000134         8903 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJYC010000134 DAMJYC010000000
VERSION     DAMJYC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.140"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            92..658
                     /locus_tag="PC472_003550"
     CDS             92..658
                     /locus_tag="PC472_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2728847.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            963..1424
                     /locus_tag="PC472_003551"
     CDS             963..1424
                     /locus_tag="PC472_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2728848.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1492..3738
                     /locus_tag="PC472_003552"
     CDS             1492..3738
                     /locus_tag="PC472_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728849.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3731..5563
                     /locus_tag="PC472_003553"
     CDS             3731..5563
                     /locus_tag="PC472_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728850.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6282..7559
                     /gene="hflX"
                     /locus_tag="PC472_003554"
     CDS             6282..7559
                     /gene="hflX"
                     /locus_tag="PC472_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2728851.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7792..8289
                     /locus_tag="PC472_003555"
     CDS             7792..8289
                     /locus_tag="PC472_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2728852.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8338..8862
                     /locus_tag="PC472_003556"
     CDS             8338..8862
                     /locus_tag="PC472_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2728853.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         3519 a          897 c         1624 g         2863 t
ORIGIN      
        1 ttaataaata taaattaaat tatatgtgta tatattgtac tttaatagtg atataataat
       61 aaaaaaattt ttaaagaggg tgatattaca tatgagaaaa cttgaagaga tattagaata
      121 taataaatca tttgtagaga acaaagaata tgaacaatat gtgacatcaa aacatccaaa
      181 taaaaaaata gtagtcttat cttgtatgga tacaagatta actgagcttt tgcctaaggc
      241 tatgaatttg aaaaatggag atgtgaaact aataaaaaat gcaggagcta ctataatgca
      301 tccttttgga agtattatga gaagtgtttt agttgcaatt tacgaatttg atgtagatga
      361 ggttatggtt gtagggcatc atggatgtgg tatgtgtaat gttgatacgg ataagttact
      421 tggtaaaatt ttagatagag gaatatctaa tgatgtaata cttacacttc gtagtgctgg
      481 aattgataca aaacaatggt tacatggatt cgattctgca gaagaatctg taaaagatag
      541 tattgattta ataaaagggc atcctttaat tccagatggt attattgtac atggtctaat
      601 tatatcacct gaaactggga agttagacgt tgttgtaaat ggatatgaag ataagtagag
      661 tatcataatg ttatataatc aagatactgt tgaatatgat gttgtttcaa aatttatttt
      721 gggacaactt ttttatttga aaatatatga aaaggtttat tttaagcttt acaataaaaa
      781 atataagtag ctgactaatt aaaaatctta ctattattta ttaaaattat aactatatga
      841 tataaaaata tttaataaat gaaatttata tgaatttttt ataaatatat tgaaaaaatg
      901 catgcagtat agtaaaataa tgcttagccg actaagtaat ataaaaaata aggagggcaa
      961 gtatgaatag caattctgaa gaaaaattaa atgatgtttt cttgcaatta atgcgaatat
     1021 attttataag aactcatttt tattttgata aattaggctt atatcatggt caaccccaaa
     1081 tacttttttc tttaaagatg aaagatgggc aaagtcaaaa agaattagca gataaaagga
     1141 aagtaaaagc atctacaact acagttatga taaagaggat ggaaaaagct ggacttgtgg
     1201 aaagaaaaca agatgaaaag gaccaaagag tatctaggat ttttttgaca gacaaaggtt
     1261 tagaaatatg taataaagtt gaagaattta acaaggaatt ggaaagagag ttttttttag
     1321 gatttagtga agaagaaaaa atagtactta gaagacttct aatgcaagta cgagataatt
     1381 taaaagcagg atgtcataag catgaagatg aaaaatattt ttaaagttta aaataatgtt
     1441 tttcaattta atgttattag actaattaat attacagaag ggaagaaatg tatgtttaaa
     1501 ttaattaaat atttaaagaa atcggcatta tcaatagtaa taatagtctg tctattggta
     1561 attcaagcgg tatgtgacct ttctttacca gaatacacat ccaatatagt aaatgtaggt
     1621 atacaacaag gtggagtaga aaactctgtt ccatctgtga ttagagagag tgaattaaat
     1681 aaaattactt tatttatgga taaaagtagt aaggataaag tactagataa ttatactttg
     1741 ctgaataaaa aggattatgt taagtataaa gataagtatc caggtctaaa agatgaatct
     1801 ctttatgagt taaatactaa agacaaagac actatagatg atttaaatgt tatttttggt
     1861 aaagctattt taatagtatc agggcttgag ggcgatagta aagaggtaaa agctatgaaa
     1921 gctcagttaa tgagcaaact tccaccacaa gctactcaaa gtggagatgt tgatatattt
     1981 aaattgttat cagcaatgcc aaaggaacag ttagatacat tgactaaaga gatgagcaaa
     2041 ggctttgaaa gtatgccaga gagtatgata acacaaagct ctgtttcata tgtaaggtca
     2101 gaatatgaga aaataggtat tgatacagaa aaaactcaaa ataattatat attatttact
     2161 ggtgctaaaa tgcttggtat agcaatgatt agtatggtag ctacaataac agtaggattt
     2221 ttagcagcaa gagtagccgc aagtgtaggt agaaatttaa gaagtggagt atttagaaaa
     2281 gttatgtcat tttcaaatac tgaaatgaat gaattttcta cagcttcatt aattactcgt
     2341 agtacaaatg atattcaaca gatacaaatg ttgatggtta tgttgcttag aatagtattc
     2401 tatgcaccta taatggctat aggtggagtg ataaaggttt taaacacaaa tacttcaatg
     2461 gcctggatta tagcagtagc agtagttgct atcttatcat taattgtagt gttattctca
     2521 gtggttatgc ctaagtttaa actggttcaa aagcttgttg ataagttaaa cttagtaagc
     2581 cgtgaaataa ttactggtat tccagttatc agagctttta gtactgaaaa acatgaagaa
     2641 gaaagatttg ataaagcaaa taaaaattta actaaaacaa atctttttgt aaatagagtt
     2701 atgacatgta tgatgcctgc tatgatgctt ataatgaacc ttatcacagt tcttatagtt
     2761 tggagaggtg catacagtgt tgattctgga gcaatgcaag ttggagatat gatggcattt
     2821 atacaatata caatgcaaat aataatgtca ttcttaatga tatcaatggt atctattatg
     2881 cttccaagag catccgtttc agctacacgt atagatgaag tattagtgtc agatataagc
     2941 ataaaagacc cagaaaaatc agaggcacaa gagtttaagg aagataaaaa gggtcttgtt
     3001 gaatttaaaa atgtttcgtt catgtatcca gacgcagaag aacctatatt aacaaatata
     3061 tcttttacag caaagccagg agaaacaact gcctttatag gaagtactgg tagtggtaag
     3121 tcaactctta taaatttaat tcctcgtttc tttgatgtta ctgaaggaga aatattactt
     3181 ggtggagtta atataaaaaa tgtttctcaa catgatttaa gggaaaaaat tggatatgta
     3241 cctcaaaagg gagtattgtt ttctggtact atagaatcca acttaaaata tggtagaaaa
     3301 gacgcaacag atgaagaaat gagaacagca gctgaaatag ctcaagctac agaatttata
     3361 gattctaaac cggaagcttt taaaactgaa atttctcaag gtggaacaaa tgtatctgga
     3421 ggtcaaaaac aaagactttc tattgcaaga gcaatagcaa aaaatccaga agtttatata
     3481 tttgatgata gtttttcagc acttgactta aaaactgatg cagctcttcg taaggcttta
     3541 aaatcaaaaa ctgctgaaag tacagttcta atagttgcac aaagaattag tactatctta
     3601 aatgcagaac agataattgt tttaaatgag ggcgaaatag ttggaatagg aacacataaa
     3661 gagcttctaa aaaattgcga agtttatgag caaatagctt tatcacaact ttcaaaggag
     3721 gagctagcta atgagtagaa tgggaagagg tcctatggga aaatctatgg gagcaggaca
     3781 aaaagcaaat gactttaaag ggactatgcg aaaactaatt gcatatttat ctaaatttaa
     3841 aatatcaata attctagtaa ttgtatttgc tattggtagt gcgtcgtttt ctatagtagg
     3901 tccaaaaatt ttaggaaagg caactacaaa gatatttgaa gggctagtga gtaaagtttc
     3961 tggaggaaac gtaggtatag attttaatgc aattggaaaa atcttaacct ttttactttt
     4021 cctttatttg ataagtgcac tgttctcttt tatacaagga tttataatga gtggaatttc
     4081 tcaaaaagta tcctataatt taagaaaaga gatttcagca aaattggata gattgccaat
     4141 gaagtatttt gatactaaga cgcatggtga aatattatcg agaattacga atgatataga
     4201 tactttaaat caaagtttaa atcaaagtat gacacagctt ataacatcag taacaactat
     4261 gataggtgtt ttgataatga tgttatctat aagtggaatt atgacattag tggcagtttt
     4321 aatattacca atatcaatgt ttgtaatttc aagaataata aagaaatcac aaaaatactt
     4381 tagatatcaa caagaatatt taggaaatgt aaatggtcaa gttgaggaaa cttacagtgg
     4441 ccaaacaata gtaaaagcat ttaatcgtga ggatgaagta attgaagagt ttgataaact
     4501 aaatgatagc ctgtataatt cagcgtggaa atctcaattt ttatcaggta taatgcaacc
     4561 acttatgatg tttattggaa acttgggata tgtaatggta tcaatcttag gaggttggct
     4621 tgcaattaag aaaacaattg aggttggtga catacagtca tttatacaat atgtaagaaa
     4681 ctttacacag ccaatgactc agattgcaca ggttgccaat ttattgcaat caacagcagc
     4741 agcttctgaa agagtatttg agtttttaga agaggaagaa gaagtccaaa ttgttgaaaa
     4801 tgcagtttca atagatggtt tagaaggtaa aattgatttt gaaaatgtca actttggata
     4861 taacccaaac aagactataa taaatgattt ctcagtaaat gttaaacctg gtcaaaaagt
     4921 tgctattgtt ggacctacag gagcaggtaa aactacaata gttaaactgt taatgagatt
     4981 ttatgatgtt aatagcggtt caatactgat tgatggtcat aatattaaag attttaatcg
     5041 tagtgagctt agagaaatgt ttggtatggt tcttcaagat acatggttat ttagtggttc
     5101 aattatggaa aatataagat atggtaagtt gaatgctaca gatgaagaag ttatagaggc
     5161 tgctaaatct gcacatgttc atagatttat taagacatta ccagatggat ataagatgaa
     5221 attaaatgaa gaagcaagta atgtttctca aggtcaaaaa caattattga ctatagctcg
     5281 tgctatttta gctgatccta aaattttaat attagatgaa gctacaagtt ctgtcgatac
     5341 tagaacagaa gtattgattc aaaaagctat ggataatctt atggaaggta gaacaagttt
     5401 tgttatagcc cacagattat caactatacg tgatgctgat atgattcttg taatgaatga
     5461 aggagatata gttgaacaag gaaatcacga ggaacttcta aagaaaggcg gattctatgc
     5521 caatctttac aattctcaat ttgaagaaga tgaagcaatg taacaaaaaa ctatcttagc
     5581 atagacatgt aattaataag ataaaataaa tgagggtgcc ttaaaatgaa ctttttaatt
     5641 cataaggcac tcttttgtgt atgaaataaa gtgtattttt atcattttaa caatgaaaaa
     5701 gaggattctc tatattttga gatagccaca taattttatg tctttacaaa gtgtttattt
     5761 tattataata tgtagtaagg aaaatgttgg aggtgattat caaaatgtat atttaatttt
     5821 attctttaaa ttttattata aatagaattt aattttaata gttgatgatt atattgaagt
     5881 atatgttttt attactttta ttaatcatgc aaataactac ttaaatttaa gaatagggtt
     5941 aaatataaaa tgataattac atataagtta attaaactaa tgtattttta atatatattt
     6001 agaattttaa tccttcttat gatataagta gcttgttttg tattacttag atttgtaagg
     6061 agttttttta ttgaaaaaac tgcataatat atttgggagg aggtgtaaga aagtgatttt
     6121 ttatataaga ttgagaaaat aatgcacgat tatggttatt ataatagtaa ataagaagtg
     6181 tcatttaagg ataagataaa aagcaaaatt cttgatgaat ttaattatag agccataatc
     6241 tcgtgctata gattaaataa aaaatagcag ataaggagaa tatggatatg gcgaaaaaag
     6301 gaataacagt aggattaaac ataaataata aatctgagga ctttaatgaa ctaatgattg
     6361 aacttgaaaa cttatgttca gcatgtgata ttgatgttgt agggagtata actcaaaatg
     6421 caaagcaggt taatagagca ttttacattg ggacaggtaa agttgaggaa atattaaact
     6481 taataaagaa agaaaatata gagatagtta tattttacaa tgaactttct acatcacaac
     6541 taaaaaatct tgaagaaaag cttaattgtg aaattataga tagaacggca ttgatacttg
     6601 atatatttgc acaaagggca aaaactagag aagctaaact tcaagtagaa gtagcaagtt
     6661 taaaatatat gttaccaaga cttataggtt caaatgaaaa tttaggacgt caaagtggtg
     6721 gagttggtac taaaaataga ggttctggtg agaaaaaatt agaacttgac cgtagaagaa
     6781 tagaagaaaa gataacatct ttaaataggg agttggatga tttaaaattc caaagagaaa
     6841 ctcaaagaag tatgagaaga aaatcaaatc ttccaaatgt agctctagtc gggtatacaa
     6901 atgctggtaa atcttcaata atgaataaac tagtagatat atttaaaaac tctgaagaaa
     6961 agaaagtgtt tgagaaaaac atgttatttg ctacacttga aacttctgtc agaaatatag
     7021 ttcttgcaaa taacaaagaa tttttattat ctgatactgt tggttttgta agtaatctac
     7081 cacatgactt agtaaaagca tttaggtcaa cattagaaga agcatgtgag gcagacgtac
     7141 tgttacatgt tattgatatt tcaaatccaa gctataaaag tcatattaaa gtgacagaag
     7201 atactttaaa acaaataggt gcagatggta tacctatgat acatgtgtat aataaaattg
     7261 atttaataga tgtagaagta ttggatagaa ttttagatag tatcgataaa gaaggtatat
     7321 ttgtttcagt taaaaaagat ataaatatag ataaaatgat aaaatgtatt tgtgatagca
     7381 tatttaaaga ttatgtaaga tgtaagttct taattccata tgataaaggt catgtagttt
     7441 cttattttaa cgaaaatacg agcattataa atacagaata cagagaagat ggagctattt
     7501 tagatgtaga atgtagcaat atagaatata ataagtataa aaagtatgct ttagagtaaa
     7561 ttttgaaaaa cttattatag cctaaaatat atttaattta taatttatta taaattgatt
     7621 gtaaatatga ttttatgaaa cggtatagcc ataatgctat gccgttttgc cgtttataat
     7681 aaaaataaat ttattgacat aatagtaact aattactata atataaagta attagttact
     7741 atttaaaatt atcaaaaaat taaaatctaa gataaagaga agtgtataat aatggatgca
     7801 gaaagtttaa tgaaaaaata tatggtagat gatgatagta gaaaacaagc tatatttgca
     7861 agtatatttg taatgcaaaa taaacttcaa actacatgtg ataaattaga cccagaatta
     7921 acaatgaaac aatggttact tttagctatt gcggactctg tagatgaaac tcttacatta
     7981 accaaacttg gagaattaat ggggtgttca agacaaaatg taaaaaaact agctattgct
     8041 ttagaaaaga aaggttttat agaaattgag caaaatgaaa aagattcaag agctatctgt
     8101 cttgtgacaa aagatagagt ggaagagtat tctaaaaaag tgagaggtat gcaggaaaaa
     8161 gtattacagc ttttatttga agattttaca gagaaagaag cggaacagtt attttttgga
     8221 attatgaaat taagtatagg aattgaaaat gtgaaaaatt atgtagaaaa taatgacgtt
     8281 ggagaataag caagtatcaa atcaactttg tatgacatca gagaggggag aatttatatg
     8341 aaaagtgtag tagtgattta taagagtaag tatggttcaa caaaaaaata tgctaaatgg
     8401 ctttcagaat tattgtcttg tgatattttt gaaactaaga atattaatat agaaaaggtt
     8461 atgaagtatg atacgattat tttaggagga ggtctttatg cttgtaatat agcgggaatt
     8521 tctttcttaa aaaaaaatta taaagaattg cagaaaaaaa atttagtagt atttggtgta
     8581 ggagctgctc catatgatga tgaagtaata aagaaattaa gagaagtaaa ttttaaggat
     8641 gaattaaaca aaattccttg cttttattgt cgtggggcat gggatgagac aaaaatgtct
     8701 tttatagatt atactctttg tagttttttt aaaaaatcta tagctaaaaa agaacgtcaa
     8761 ggtcttaaac cagcagaagc acctttattg tctggagaag cctttgattg gtgtaataaa
     8821 gaaaatttaa ctccaatact tgattatata agagaacttt aagaggtata tatttgtaat
     8881 tatttattat ctaaagttag att
//
LOCUS       DAMJYC010000135         8637 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJYC010000135 DAMJYC010000000
VERSION     DAMJYC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.141"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            16..2193
                     /locus_tag="PC472_003557"
     CDS             16..2193
                     /locus_tag="PC472_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2728854.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC472_003558"
     CDS             2207..2629
                     /locus_tag="PC472_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2728855.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3037..3840
                     /locus_tag="PC472_003559"
     CDS             3037..3840
                     /locus_tag="PC472_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2728856.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3833..4501
                     /locus_tag="PC472_003560"
     CDS             3833..4501
                     /locus_tag="PC472_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2728857.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4737..5399
                     /locus_tag="PC472_003561"
     CDS             4737..5399
                     /locus_tag="PC472_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2728858.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAIGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5459..6256
                     /locus_tag="PC472_003562"
     CDS             5459..6256
                     /locus_tag="PC472_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2728859.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6374..7564
                     /locus_tag="PC472_003563"
     CDS             6374..7564
                     /locus_tag="PC472_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2728860.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7673..8422
                     /locus_tag="PC472_003564"
     CDS             7673..8422
                     /locus_tag="PC472_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2728861.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3430 a          864 c         1592 g         2751 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta taaacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttcgccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcacca tcaaaatgga
     1021 tttcaaaatt atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca
     1441 tcatttgaga gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gctatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg
     1621 tttaaaaata catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggttca tatacaagag aagggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca
     1801 ctaccaacat atagaggatt taatgataat atagatgatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tctagcttag atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaagctgat
     2101 ataaatagta ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgtaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg
     2401 tgtatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agaatgactt atacttaggt ataagatatt tttacaaaaa gctgtattga gtttgttata
     2701 atgaaaactc aatatagctt ttttatggat atagttggat actataatgt gattcttagt
     2761 aatagttttt tgctattgga caaagactgt aaaacatatt aaacttatgt tgttcaaagt
     2821 cgaagtataa tataataact agaaaattgg tttatagtgc tataaaagca aatttagtaa
     2881 ggtataaaat cagaggcaat cctttactat aatatcaata tttgggagtt ctgttattgc
     2941 agagctatta aacaattata atggtacaag tgattcaacc tttgaaaatg tattatataa
     3001 aaaattaaaa taagattaaa aaggagagaa taaattatga aaaaacttaa attaattatt
     3061 atgtttgcct tggtatcagc actgttagtt acaggatgtt catcaggaaa taataaaaat
     3121 aaagaagaac aaaaagttat aagagttgca acatcaggaa catattaccc ttttgctttt
     3181 aaagataaag ataaacttaa agggtttgaa gtagattttt gggatgaatt tgctaaacga
     3241 actaattgta aagttgaatg ggtattaact gattttagtg gattatttgg attattagaa
     3301 gctgataaag cagatgttgt atctgctcaa cttactgcaa cacctgaaag agagaaaaaa
     3361 tatgcttttt cagatgctta taattatagt ggaacaaaca ttattgttag agaagatgat
     3421 actagtataa aatcaataga tgatttagtt ggcaagaaag taggtgtggg tactggagca
     3481 gtagcaaatg aaatattaaa agaaaaatac ccaaatgatg aaataaaaat agtaaattat
     3541 tcaagtgcga ctcttaaagg gaattatcaa gacctagaaa tgggaagatt agatgcagta
     3601 gttgcgcagg atgttgaggc tttaatagct ataaaagaac aaaaattaaa tcttaaaatg
     3661 atagaacctc caattcaatt tggtgcctgt tcttttgcgc ttcaaaaaaa tgaaaaaggt
     3721 gaaaaatgga caaaagaaat taacgaagta attgaagaga tgtataaaga tgggactttg
     3781 actaaaatat ctgaaaactg gctaggaaaa gacattacta aggagcctat taatgagtaa
     3841 ttttaatttt gaattttgtc taagtatatt tccatttttg attaaagcat tgggaataac
     3901 aatactaata tctattgttg catttttttt atcaatatta ttaggtgtaa tacttgcctt
     3961 tttcacacag acaaaatcaa aagttttaca atacataaca agagtgtatg tttcattctt
     4021 tagaggaaca ccacttttaa ttcaaatatt tttcttttat tttggatttc caatgatatt
     4081 ttcttttatg aaagattgtt caccttattt agcaatagta atttgtttag gtgtaaattc
     4141 atctgcatat atggctgaaa ttatacgagc atctattgag agtgttgata aaggacaaca
     4201 ggaagcatgt tatgcttttg ggttaagtaa atatcagtca atggttaaaa taattctacc
     4261 tcaagcagct acatctgcag tacctcctat tgtaagctgt acactggaca taattaaagg
     4321 ttcttcttta ggtttaacta taggaataca agatttaatg ggagtagctc aagtagaggc
     4381 tgcttcaagt tttaaatatt ttgagatata tatgataacg atattaattt actgggcagt
     4441 atctataata ttgagtaata tacaaagcaa agttgaaaaa agaataagaa gagcatatta
     4501 gatttcaata tttaaaaatg ggatgatata gatatattat tacttgtaaa taaattttta
     4561 attttagaaa aataaactta gtaatctaaa ttattgagaa aatatagtat caaaatggac
     4621 tgtataatca agttaaaaaa tagtttttct attgaaaaaa taaataaaat tctattgtaa
     4681 tttaatatgc gataaagttt tataaaaaac aacattaagc tagggggact tattttatgg
     4741 cttttaatat gaaatatttt ttagaattat ttccaacaat aatgaaatcg ttcaatgtaa
     4801 ctgtgatttt ggcaataagt tcattagtat tttcattaat tataggtaca atagtagcgt
     4861 tatcatctta ttacaaagtg aaagtattga atcaaatatg taaagtatat gtatctattt
     4921 ttagaggtac acctcttatg ccacagttat tctttttata ttttggacta gcatatatga
     4981 gtgattttgt taaaaatatg gacccagtat ttgcaacatc tatagtactt agtttaaata
     5041 tgggagctta tatgtctgaa acgataaggg catctatact ttcaattgat acaggtcaga
     5101 tagaagcagc atatgcaata ggattaacaa atttacaaac aatgaaaagg atagtgatac
     5161 cacaggccgt aagagttgca ctgccatcat tattcaataa ttttatagat ttaattaaag
     5221 ggtcttctgt agcttttgtt gttggagtaa atgatataat gggtgctgcc aaatcacaag
     5281 gagcgttgag ttttaagttt tttgaagtgt atggagcagt tatggtaatt tactggtgta
     5341 taataactcc tttaggttgg gggcaaaaaa aattagaaaa gaaaataagc aattattaag
     5401 ttattaagaa aatacataaa agtattttta tataaaaaat ggtgtgagga gaagagatat
     5461 gataaaaaga aatattataa aatgtttagt ggcagtttta tcgttaacgt ttgtaattac
     5521 tggttgttca aaaaaagagg aagtaaagaa tgaaaaagtt ataactgtag cttcatctaa
     5581 ttcagctgac ccatattctt atgttgataa tggtgagcat aagggttttg aagttgacat
     5641 gtggaaagag attggtaaaa gaagtggata taagataaaa atggaaccaa caggttttag
     5701 ttctatattt ggtatgttag attctggtaa agttgatgtg gcagccaact tctttggaat
     5761 gtctaaagaa agattagaaa aatacgatgc ttctatacct tatggttctg attctgtgtg
     5821 tatagctgta aaagatggaa ataacgaaat taataagctt gaagatttaa agggaaaagt
     5881 tgtagcagtt tcagaaggtt ctcaaggtca acaggttgca actagtgtta ataaagataa
     5941 tttgtttcaa gaaaaggttt acggtgatgg aactagtggt cttcaagatt tagatttggg
     6001 aagagttagt gcatggattg atgcagagct tactattgtg ggagacgcta aaaaagctaa
     6061 tatgaaagta agagtattag ataaaaaatt atcagctact aatatagcat acttctttaa
     6121 gaaaaacgat gataaatcta aaacgataaa agaagatgta aacaaagcaa tagaagaaat
     6181 gcttgcagat ggaacagtga aaaaaatatc tgaaaaatgg tttggaatgg atgtaagtgc
     6241 tgatatacaa aaataaatac tttaaaatta aaatgtaaaa ctgtgataac tatagataag
     6301 tatactaaat ttatctatag ttatctgtgt attacaatgt atttagatta gtctttataa
     6361 atcaaaatga gttatggagg tattgcaaat gattgaaact aaaataaaat ccattgcact
     6421 aaaaaataga gaaaaaatta taaaaataag acgacagata cacagtaatc cagagttagc
     6481 atttaaagaa tataaaacat ctaaacttat aaaagaagag ctaaataaac ttaatataga
     6541 atacataaat attgctggta caggagtatt agctactata aaaggaaaaa ataatggtgg
     6601 aaaaactata ttactcagag cggatatgga tgctcttcca atcaaagagg aaaatgattt
     6661 agaatttaaa tcaataaatg ataatatgca tgcttgtggg catgatgcac atgtttcatg
     6721 gttgttagga acagcaatga ttttaaatga cataaaagaa gaattgaatg gaaatgtaaa
     6781 attactattt caacctggcg aagaaaaagg tggttctgat attgtaatta aagaaaatgt
     6841 acttgaaggt gtggatgcat tagcgactgg tcattgttgg ccaactattg agtcaggaaa
     6901 aattggaatc gctagaaatt gtgctatggc agcgactaat acttttgaaa taacaataat
     6961 tggcaagggt gggcatggag cagagcctca taattgtata gacccaattg cagttggaaa
     7021 tgcagtatat tcatccattc aacagatagt ttcaagaaaa attgacccag ttgtacctgt
     7081 agtagtgtct gtttgttctt ttaattctgg tgtttctaaa aatataatac cagatgtatg
     7141 tacacttcaa gggactatta gagcgataag caaggaacaa gtaattgaag taagtaaaat
     7201 tctagaatat atagtaaaaa atgtatgtaa agcacatgga gcagattgca aatttgaaaa
     7261 gagtacagga ggagatgctg ttataaatga caaaaacatg atagagatag gaagagaaag
     7321 tgcttgcaaa atacttggag ctgaaaatgt tgaaataatt gattttcctg caatgacagg
     7381 tgaggatttt gctatttata tgaaagaaaa acctggttta tttatgtata taggtgtagg
     7441 agataaagaa aaaaatataa attacagatt gcatagtaac aagtttaata tagatgaaaa
     7501 atgtcttagt atagcacctt cattattttc acaacttaca gtagattatt tatgtcaact
     7561 atagagtaca ttcataaaaa tatatttagc ctatttttaa atttaatgct agtatattaa
     7621 ttttgatgta gaacatatta tttattatat ttatttaaaa aggtggataa caatgataaa
     7681 agtagaaaat ttacataaaa gttttaaagg gacagaagtt cttaaaggta taaatttaga
     7741 gataagtcaa agtgaagtag tagctatact tggatcatct ggaacaggta aatctacgtt
     7801 acttagatgt ttaaattatt tagttgagcc tacaaaagga aagataatga ttggtgatgt
     7861 aaaagtagat gtagaaaaag caacaaaaaa agatataacc aatttaagaa aacatacatc
     7921 tatggtgttt caaaattata acttatttaa aaataaaaca gcattagaaa atgtaatgga
     7981 ggcattgata gtagttcata aaaagagtaa gaaagaagct agagaaatat ctttaaaatt
     8041 aatagataaa gtaggtatgt tggataggaa agatttttat ccatcaaagt tgtctggggg
     8101 acagcaacaa aggataggta tagcgagagc gatggcagta aatccaaatg taatattatt
     8161 tgatgagcct acatctgcac ttgacccaga acttgttgga gaagttttaa gcgttataaa
     8221 atctcttgca aaagaaggta taacgatgct tattgtaact cacgaaataa attttgcaaa
     8281 agaagttgct gataaaatta tatttatgga taatggtgta atagctgaag aaggaactcc
     8341 tgatgaaata tttaataatc ctaaaaatga aagaacagca aaatttttaa atatcattag
     8401 gaaagaagaa gtatttagtt aaaagtgtat aaattaagca ttagaagata tgtaagatac
     8461 ccccattaaa tttataagtt agataataaa tttaatggga gtatattttt attattagat
     8521 ttattattta atttaaaatt agaaatgtaa ctatttgaaa attcttttta aaagtatatc
     8581 aaaatatcaa atacataaaa aaataatatt tgacaaagta tcatatgtgt ttacaaa
//
LOCUS       DAMJYC010000136         8624 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJYC010000136 DAMJYC010000000
VERSION     DAMJYC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.142"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            85..1575
                     /locus_tag="PC472_003565"
     CDS             85..1575
                     /locus_tag="PC472_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2728862.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1855..3351
                     /locus_tag="PC472_003566"
     CDS             1855..3351
                     /locus_tag="PC472_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2728863.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3485..4411
                     /locus_tag="PC472_003567"
     CDS             3485..4411
                     /locus_tag="PC472_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2728864.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4529..4720
                     /locus_tag="PC472_003568"
     CDS             4529..4720
                     /locus_tag="PC472_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728865.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4943..5293
                     /locus_tag="PC472_003569"
     CDS             4943..5293
                     /locus_tag="PC472_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728866.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            5554..7059
                     /locus_tag="PC472_003570"
     CDS             5554..7059
                     /locus_tag="PC472_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2728867.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            7449..8546
                     /gene="dinB"
                     /locus_tag="PC472_003571"
     CDS             7449..8546
                     /gene="dinB"
                     /locus_tag="PC472_003571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2728868.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
BASE COUNT         3419 a          875 c         1609 g         2721 t
ORIGIN      
        1 ataatatttt atgtatatat taataaaagg acatattgtg ttataatggt atcgtataaa
       61 atcatttacg ggggtggtat taaaatgttt caatttgaaa cacaaataca caaattaaga
      121 catgatgttt taactgcaat agctagactt ggaaaagaag ataaccttac cagagaagca
      181 atggataata ttcagtatga aataataaaa ggtgagaaac ctacatacag atgttgtgtt
      241 tataaagaaa gagctataat atctgagaga gtaaatgtaa gtatggggtt acaaccaggt
      301 caagataaga tagatttaga tacaatggat atagaagaag atgaacaaat tatatatatt
      361 ttggaagctg catgtgacag ttgtccaata aatgaattta cagtaacaga tatttgtaga
      421 ggatgtctag cacatagatg taaagaagct tgtaaatttg gagctataac acatgtcgga
      481 ggaatggcct atataaatca tgaattatgt aaggcttgtg gaatgtgtaa aaaagcttgt
      541 caatatgatg ccatctcaga ggttgttaga ccatgtaaga gtgtttgtcc aactaatgcc
      601 ttaggatttg atagagaaaa tatgaaagct atgatacatg aggataaatg tttaaactgt
      661 ggagcatgta tgtcagcatg tccatttgga gcaatatctg ataagagttt gatagctcct
      721 gtagctagaa agttagttca aaaagagaaa atgtatgcgg tggtagctcc agctataaca
      781 ggtcaagttg aagcaaatgt tacatatggt caaataaaaa atgctataaa atcactagga
      841 tttgttgatg tattagaagc agcttgtgga gcagatgcag ttacagttca tgaaagctta
      901 gaatttgctg aaagaatgga aaagggagaa gcttttatga caaattcttg ttgtccagga
      961 tttgttggtt atatagaaaa aacataccca aatcatgtag ggaaaatatc ttctacagta
     1021 tctcctatga tagctagtgg tagatttata aaaagtcatg aaccagatgc taaagttgta
     1081 ttcataggac catgtacagc caaaaagagt gaggcagcta agaaagaatt aaaaggagct
     1141 gttgattatg tacttacttt tgaagaaata atggcattat ttgaggcatt tgatatagac
     1201 ttggctaagt gttcaaatga agatatagat gatgcatcaa tttttggaag aggttttgga
     1261 gctgctggtg gtcttacaaa agctatagaa aattatcttg ctgaaaaagg tattggtgtt
     1321 aattttgaac ctgtaaagat ttctggttca agagaaatca agaaaactat gactatggct
     1381 aatttaggaa agctagaagg taattttata gaaggaatga tgtgtgaagg tggttgtata
     1441 aatggttctg gtaaattctt atcaggtgca aaggttaaag ccacttttga tagaaaaaat
     1501 tctcaatcaa ctaagaaaag tgtcttagca aatacaaaga taaaagattt tagtaatata
     1561 aatttagaaa aataaagata atacaaagca acaaaaatac atattgttaa tagagttgtt
     1621 ctaagttgag gtttaacttt tggacaactc tatttttatt tatatattta attattgaac
     1681 aactaaaata aaaattatat acacttaaaa taaaaattac atatataata ttttgtatat
     1741 aacttaacaa agtatatgcc tatgttataa taatttatag aaataaataa aaaatacaat
     1801 atacataata gtagaaatat agcaaaaagg tgtgatttat agggaggatt acttatgttt
     1861 gaatttgaaa cgcaactaaa aaggttaaaa catgaagttt taacagcagt agcaagacta
     1921 tctattgaag aaaatttaac aaaagaaaat atagaaaaaa ttccatatga aataattaaa
     1981 ggagaaaagg cgagatatag atgttgtgtt tataaagaga gagaagtagt tcttgaaagg
     2041 gcgcaacttg cattaagttt aactccaaat agtaaatatg gagatataaa tccagattca
     2101 gttagtttgg acacagatga gcaaattata tatatagttg aagcagcatg tgatagatgt
     2161 ccaataaatg aatttacagt tacagagctt tgtagagggt gtttggctca tagatgtaag
     2221 gaagcatgta aatttggggc aatttcttat ataaatggga gagcatatat aaatcatgat
     2281 ttatgtaagg cttgtggaat gtgcaaaagt tcatgtcaat atgatgctat atcagaagtt
     2341 gtaagacctt gtaaaagcgt ttgtccaaca ggagctttgg attttaatag aaacactatg
     2401 aaagcaatga tacatgaaga taattgtata aattgtggag catgtatgtc agcatgtcct
     2461 tttggagcaa tatctgataa gagtttgata gcacctgttg caaaaaaact tgcaaagaaa
     2521 gaaaatatgt atgcaatagt agctccagct ataacaggtc aaatagatag taatattaca
     2581 tatggtcagt taaaaaatgc tataaaatca ctgggatttg tagatatgat agaagcttct
     2641 tgtggagcag atgcagttac agttcatgaa agcaatgaac ttattgaaag aataaaatca
     2701 ggagataatt acatgacaaa ttcatgttgt ccgggatttt ttaagtatat agagaagatg
     2761 ttcccagatg aaaaagatag aatatcatct acagtgtctc ctatggtagc aacaggaaga
     2821 ttagtaaaaa aaatagattc tgatgctaaa gttgtattta taggtccatg tacagctaaa
     2881 aagagtgagg caacagagaa aagtttaaaa gattcagtag attatgtact tacttttgaa
     2941 gaactaatgg cattatttga agcatttaat atagatttat caaaatgttc aaatgaagat
     3001 ataaatgatg gttcgatttt tggaagggga tttggagcat ctggtggact tgccaaatca
     3061 gtacagaact atattgctga aaaggggctt agttttgatt ttgaacctat aaaagtatct
     3121 ggtccaagac aaattaaaaa gacaatgaca atggctaaat tgggaagatt agaaggtaat
     3181 tttatagaag gaatgatgtg tgaagaaggt tgtattaatg gagccggtaa gtttttatca
     3241 ggtgtaaaag ccaaaaatac ttttgataga aaaaatggac aatctactaa aaagagtgtt
     3301 ttatctaatg ataagattaa agaatttgaa gatgtaaatt tagaaagata attgttttgg
     3361 tacagtcatt tttttattat gccacaaata tgcctaaaat ttgccctttt tatatgtgta
     3421 ttttacagag ttaaggttgc tatactataa atatgtaata aataaaattt ataggagctt
     3481 ttggatgagg aaaatagtat taaaagatat cctgttttta gttactacat tatctataat
     3541 agtaatatta ttgcttgtaa caaatataac tactaatagt aagataaaat caacaagtga
     3601 atcttattta aaaagtgaca aaaatagtgt gaggcaaaat ggtatccaga ggagcacaaa
     3661 gagtagcaca aaaaatactg catttaagac tactatacta aacactggaa agtctgattg
     3721 catattggtt gagataggta acaaagtcat aatgattgat acaggagaag ataaaaatgg
     3781 aaaacaaata gtggataggt taaaagaaaa aggaataaat acacttgact atttgatttt
     3841 aactcattta gataaggacc atataggtgg agtagatagt gtgttatcta gtgttaaagt
     3901 aaagaatatt atacaagcaa attataagaa agatagtaag caatatgatg aatatattga
     3961 ctcacttaaa aaagctgata tagaaccagt gctactaaaa gataatatga atattgtaat
     4021 caatggtgca gaaataaaca tacatccggc ttcaaaaagc aagtacgaga gttcaaatga
     4081 ttattcaata atcactaata taagctatgg agcacataag tttttatttg caggagatgc
     4141 agaggaagaa agattgtcag agtttataaa tggaaatact ttaaaatatg attttgttaa
     4201 aatgcctcat catggaagat atgacaaatt aactgaaaca tttttagagt ctatttcacc
     4261 tcaatatgct gttattacat gttcagagaa aaaagaacca gaggaaggtg ttttaaaaat
     4321 attggaaaga ttgaatataa aaacattttt gacttctaat ggagaggtag ttatcaatag
     4381 tgatggtaag acattgagtg tgaatcaata atttttttaa aaatatctaa aaaaatttaa
     4441 aaaaatcatt agcaaatatt accccttaaa catagtatat tttaggagct gaaactccac
     4501 atacaaaatg actaggaggt aacatattat gttggataga ttttttggtg aaggcggatt
     4561 tggtggatgt gcttggtgga taataatatt attcttctta ttcttagcat tccaagattg
     4621 ttgggctgaa atggacttat gtgcatggat acctttctta atattattat taatactttg
     4681 tgaatgtggt gacgtttttg atggaggatg tggatgctaa aaaaatcaat ttagatgatt
     4741 taataaagct tatggtgggt ataaataaaa taccaaacca atcacaatct agtgtttgaa
     4801 ggtagtcgga aatcccccgc taccttcttt tttttattgt taaaatttat ttttgaatta
     4861 tttcatttac atataataat ttttacgata aagtctgata taattaaaaa gaaaataaaa
     4921 tttatttata ggagatgtaa aaatggctat agtggataag attttcaatg agataaaact
     4981 tatggttgca agacttttat tgggaaaaaa atacagtgaa tatgaagaaa aagatttagt
     5041 gtataataca gaagaagaag ttatacttat aactttaaaa agattagttt ttcagggaaa
     5101 tgtaaacgaa gcagaagaaa ttttatttga taaagctaag agtgtaaata gtgaaaatat
     5161 gcagtatata gcaatagaat tttatactat gcttatggaa aagactgatg aagaattaga
     5221 agctatgaat ttttctaaac aagaagttta tcaaggtata gaagatatta gaaaagtatt
     5281 gaatttagaa taaaagatat atgaaaatat tgaaataaat taatgctaag gtgagaatat
     5341 tatacttata tataaaaata tagtattctt attttattag ttatataggt atatcttatt
     5401 taccataaaa ctatcttgaa aaaaattaat cttaattgta taatattact ttgagtgaaa
     5461 aagtattaac atttttttat aagatgtttt actaaaaaaa tatatataaa tatactgtta
     5521 tcaacaagaa atttaattta gtgaggtgtt aagatgaaag taggatttga ccacaaaaaa
     5581 tatttagaag agcagtctaa atatatctta gaaagagtaa ataattttga caaactttat
     5641 ttagagtttg gaggaaaatt gatgggagac cttcatgcaa aaagagtact tccaggattt
     5701 gatgaaaatg caaagataaa agtattacaa catatgaaag aaaaagttga agttgtaatt
     5761 tgtgtctatg caggagatat agaaagaaac aaaataagag gagactttgg tatcacttat
     5821 gatatggaag ttttaagatt aatagatgat ttaagaggtt atgaactaga tgtaaatagt
     5881 gtagtaatta ctagatttga aggacaacct gctacaactg tatttataaa taaattagaa
     5941 cgtagaggaa ttaaagttta taagcattat ccaacaaagg gatatccgtc agatgttgag
     6001 actatagtta gtgatgaggg atatggagca aatgaatata tagaaactac aaagccaata
     6061 gtagtagtca cagcaccagg acctaatagt ggaaaacttg gtacttgtct aagtcagttg
     6121 tatcatgaaa ataaaagagg taatgtagtt ggatattcta agtttgagac attcccagtt
     6181 tggaatgtac ctgtaaaaca tcctttgaat atagcttatg aagcagctac agttgattta
     6241 aaagatgtga atatgataga ttcattccac cttgaaacgt atggtgaaat gtcagtaaac
     6301 tacaatagag atttggaatt atttccagtg cttaaaaaaa taatagaaaa gataactgga
     6361 aaagaatcaa ttttccaatc tccaactgat atgggtgtta atagagttgg ctttggaata
     6421 atagatgatg aagtaataag aaaagcttct atagaagaaa taataagaag atactttaaa
     6481 actgcctgtg agtataaaaa gggacaagtt gataaaggtg cttatgatag aatgaaaatg
     6541 attatggaag agcttaactt aaagccagaa gatagaaatg ttgtattacc tgctagaaac
     6601 tacagtaata aattgaaaga aagtgccgac aagaatgata cttgtcctgt agttgcatta
     6661 gaattagaag atggtacaat attaactggt aagggttctg atttaatgaa tggaactgca
     6721 gcagtagtat taaatgccat taaacattta gccaatatat ctgatgatat gcacttaata
     6781 tcacctgtaa tattagaacc tatcataaac ttaaaaacaa agactttagc tagtagaaat
     6841 gtttcactta gttgccaaga ggtgttaact gcacttagta tttgtgctgt aactaatcca
     6901 actgcacaat ttgctatgga aaagttagca ttattaaaag ggtgtcaagc tcattctact
     6961 actatattaa atagagatga tgaacaaaca tttagaaaac ttggtataga tgtaacttgt
     7021 gacccagaat atccttcaca aaatctttac tatagttaaa attatataaa aaaatatggg
     7081 ctgttttaaa gtaatagttt gagcagtcca ttacaattat aataaaatca gtaggctgtc
     7141 ttgtataaga cagtcttttg tttttaaaac atcccataaa ttaaatatat atactgttat
     7201 ccataaggaa ttttaaattt agttccttgg aatacctatt gtaaagcttt tatatagttt
     7261 ttgcaagtgt ttttaaggat tttttataaa ttatattgaa gttaatggat tatagatggt
     7321 tttatttatg gaaaatatga atatataaag aaaataatga aaaaatacca aaaagaaaca
     7381 taagtttggt ataattaaaa agtaatataa ataaaattaa tctatttata ctttaaaagg
     7441 tgataataat gaaacattat agaaaaataa ttcatataga tatggacgct ttttatgcat
     7501 ctatagaaca aagagataat aaaaaattaa aaggaagacc tgttattgtc ggtggaaatc
     7561 cacaaagccg aggtgtggtt gcaacatgtt cttatgaagc tagaaaattt ggtatccatt
     7621 cagctatgcc atcagctgta gcttacaata ggtgtccata tgcagttttt gtaagaccta
     7681 gatttgatgt ttataagagt gtgtcagagc aaataagaga tatattttat agatacacgg
     7741 atttagttga accattatct ttagatgaag cttacttaga tgtaacaaaa aataaaaaaa
     7801 atattgattc atcaattgag atagcaaagc aaataaaaaa agatatattt agagaagttg
     7861 gtcttacttc ttcagctggt gtgtcataca ataagtttct tgctaagatt gcatcagact
     7921 taagaaagcc aaatgggtta actgtaataa ccgaagaaaa tgcccaggac tttttagata
     7981 aattacctgt gaataaattt tttggtgttg gaaaagttac ttcaaatact cttaaaaatt
     8041 taggtataaa aactggatat gatttaaggt gtttaaattt atttgaactt gaaaatatat
     8101 tcaaaaaaag aggttatgaa ttatataaat ttgctagagg aatagatgat agaccagtgg
     8161 aaccaaatag ggttagaaaa tctgttgggg ctgagactac attaagtcat aatttggata
     8221 ttgatgaaga agaaacacgc aacattcttg atgaactttg tgaagaagta tgtcatagat
     8281 taaaaaactc tgagaagttt ggaaaaacat taacattaaa aattaaatat gaggacttta
     8341 caaagataac tagaagcctt agtttagaac actatattga cgaatataat gatataagaa
     8401 gtggtgtaga taatttgttg agaaatgttg aggttaatgg aaaacaaatt aggctattgg
     8461 gagttacaat atcaaaccta agtgataaaa aagaaactta taaagatatt acattatttg
     8521 aatacatgga tagtatccaa atgtgagttt tgtttaaatg aaataacact agctttaata
     8581 ctatataatt gaggctagtg ttattttatg aatattttta tatt
//
LOCUS       DAMJYC010000137         8610 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJYC010000137 DAMJYC010000000
VERSION     DAMJYC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.143"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            198..1799
                     /locus_tag="PC472_003572"
     CDS             198..1799
                     /locus_tag="PC472_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2728869.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNHLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGIL"
     gene            2018..3616
                     /locus_tag="PC472_003573"
     CDS             2018..3616
                     /locus_tag="PC472_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2728870.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNISAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEIYNLVNSYRDEKGKKPLKELKSLTGMANAWSKYMYEKEIFAHEINGKN
                     AAEVFSGFGMRSGENIAYLPMNTQSIYGDKEAKEIANSIFDLWKKSSKYNDNLLKSEF
                     YSFGFGMYVSSNSEVHATMEFLNS"
     gene            3851..5683
                     /gene="slpA"
                     /locus_tag="PC472_003574"
     CDS             3851..5683
                     /gene="slpA"
                     /locus_tag="PC472_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2728871.1"
                     /translation="MKKRNLAMAMAAVTVVGSAAPVFAAASDVISLQDGTNDKYTVSN
                     TKASDLVKDILAAQNLTTGAVILNKDTKVTFYDANEKDSSTPTGDKKVYSEQTLTTAN
                     GNEDYVKTTLKNLDAGEYAIIDLTYNNAKTVEIKVVAASEKTVVVSSDAKNSAKDIAE
                     KYVFEDKDLENALKTINASDFSKTDSYYQVVLYPKGKRLQGFSTYRATNYNEGTAYGN
                     TPVILTLKSTSKSNLKTAVEELQKLNASYSNTTTLAGDDRIQTAIEISKEYYNNDGEK
                     SDHSADVKENVKNVVLVGANALVDGLVAAPLAAEKDAPLLLTSKDKLDSSVKSEIKRV
                     LDLKTSTEVTGKTVYIAGGVNSVSKEVVTELESMGLKVERFSGDDRYETSLKIADEIG
                     LDNDKAYVVGGTGLADAMSIASVASTKLDGNGVVDRTNGHATPIVVVDGKADKISDDL
                     DSFLGSADVDIIGGFASVSEKMEEAISDATGKGVTRVKGDDRQDTNSEVIKTYYANDT
                     EIAKAAVLDKDSGASSSDAGVFNFYVAKDGSTKEDQLVDALAVGAVAGYKLAPVVLAT
                     DSLSSDQSVAISKVVGEKYSKDLTQVGQGIANSVINKIKDLLDM"
     gene            5925..8267
                     /gene="secA"
                     /locus_tag="PC472_003575"
     CDS             5925..8267
                     /gene="secA"
                     /locus_tag="PC472_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2728872.1"
                     /translation="MTVLDRIVDKSDELEIKVLNYIVDDIETLEPKMESLSDEELKDM
                     TNIFKDRLKQGETLDDILQEAFAVVREVSKRVLGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPIYLNALLGKGVHVVTVNEYLAQRDKETMQPVYEFLGLSVGVITSDLL
                     PEDRKKQYNCDITYGTNNEFGFDYLRDNMVTRKSEKVQRELYYSIVDEVDSILIDEAR
                     TPLIIAGSGKEDTKLYELANAFIKTLKDKDYEIDKKENTVCLTHTGLSKAESFFGIEN
                     ITNIDNIEIYHHTNQALRAHKLMERDVEYVVKDEEIQIVDEFTGRILDGRRYTDGLHQ
                     AIEAKEGVKIKDESKTLATVTFQNFFRLYEKLSGMTGTAKTEESEFESIYKLNVVQIP
                     TNKPVIRADLQDKVFRTEDAKYNAVVEEVIKINKTKQPVLVGTVSIEKSEMLSKLLKK
                     RGIKHEVLNAKQHDKEAEIISRAGKLGSVTIATNMAGRGTDIALGAGDKIEEKKVRDI
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDEMIKLYGGKGIEKILKR
                     MNAKDEDAIESKSLSKAIERAQRGIEGKNFEQRKNVLKYDDTINEQRKVIYEERNRVL
                     NDLNISEDIQKMVREIIEDAANKYLSNQRDYYGYFKSLYNDFMPPNTLLIPGVDKKSV
                     DEVVQHTYEISKRVYDIKKMEVGIDKIEELEKRVLLQVVDTYWMDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYALEGYDMFEELNRKIRTTTVQYLYKFD"
BASE COUNT         3508 a          895 c         1651 g         2556 t
ORIGIN      
        1 atataaatat aaattcaatt ttgaatatgt ttaaaaagaa gttaaaaata attttttaaa
       61 tttaataaaa taaaacacta atatatagac gagatatatt catatcatat atactctata
      121 atatatggaa aataaagaga aaataaatgg aaaataatca caatataaat ataaaatata
      181 aaaatcatca gggggatatg aaaatgaaca aaaaaatatt atcattaggt ctagcagtat
      241 cattaatctt agtaaacttc aaaagtgtaa atgcatcatc agtagtagaa aaaatatacg
      301 gtaaagatag atatgaaaca gcagcaaaga tagctgataa acagacttat gaaacagtaa
      361 ttttagtaaa tacagaaaaa tcacttgcag atggattaag tgcaagtgga ttatcgggag
      421 ctacaaaagc tccaatacta tttacacaac aaaataagat accagcagac acaaatagat
      481 gtctaaaaaa tatcaaaaaa gcatatataa taggtacaga agatactata agtaaatcag
      541 tagaaaaaga actagattct aaaaatatag aagtcaaaag aattggtgga gaagatagac
      601 taaaaacaag ctatttaata gctaaagaaa tagcaactat aaaaaaagtg gataaagtac
      661 tattaactaa tgcatatagt ggagaagcag acgcaatgag tgtatcatca gtagctacta
      721 gagatggagc tccaatcata cttacagatg gaaagagtgt gccttttgat gtaaaaaata
      781 ttcaatcata ttgtataggt tcagaggaga taatgagtaa tcatctagtt aaaaatacaa
      841 actcagtaag aatagaagga actgaccgat ttgaaactaa caaaaacgta atagactact
      901 tttttaatag tgcagatgga ttctatgtat cagatggata ccaattagta gatgcaatag
      961 cagctgcacc acttactaaa aactctccaa tggtactagt aaatgatgga agcgataaaa
     1021 ctgtattaga aggagctaag aatataacct ctgtaggtga aataaatgag aaggtaatac
     1081 aacagtgtat aaatgcttca aagtcaaatg gacaaccacc aacaattaca gttggaagta
     1141 cagagatata taagggtgaa aagtttgaca ctggcaagtt aaatatagtg gctaaagata
     1201 acacagggaa ggtattacca atagaagttg atgggtttat agatacaaat agagtaggta
     1261 catacatatt gacattaaaa gctacagacg aatggggaaa aagtacagga aaaagagtag
     1321 aaataaaagt gttggatgac aaatctcatg attacaacag cccagaattt aaaaaaatgg
     1381 tatctactga aatgtataac ttaatcaatt cctatagaaa agaaaaaggc aaagagcctt
     1441 tggtagtatc tagtaagcta gaaggtatgg caaatgcatg gtctaagtat atgatggata
     1501 aaaaagtatt tgcacattat atagatggaa aaaatgctcc acaagtattt tctgagtttg
     1561 gaatgagaag tgaggaaaat atagcatata tttatataga ttctaagaac gttcaaacta
     1621 cacaagatgc aaaagacttg gcaaaggcta tatttgaagt gtggaagaag tctccagagt
     1681 acaatgcaaa tatgcttagt gatgagtttt atagtactgg atttgggctt tatatattat
     1741 ctgatggtca agtgcatgct actcaggagt ttttaaatgg caatgaaggt atcttgtaaa
     1801 aaattccata aatcttgttt ttttgttaat ttactgatat tataaaatga aaatcacatc
     1861 taaagtactt tttgtaatct gttttgttat tgctttacag gttatggtat tttgggtgtt
     1921 ttttgtttta gtagtttata aattttgagg gaatcactac tgaggatgta atttatgatt
     1981 agctgaatta tataaaaaaa ttagtaggag gagtttgatg aaggtaaata agagagtatt
     2041 atcaatagga ttgacaatat cattaataat ggcaggagca ccaaatatca atgcactttc
     2101 gagcatagaa aagatacaag ggaaagatag atatgagact tctgcattga tagcagataa
     2161 acaaatatat gatacagtca tattagtaaa taccgataat tctatagtag atggattaag
     2221 tgcaagtgga ttatctggag ttgtaaaagc tcctataatg ttggtgcaaa gagataagat
     2281 accaacagat gttgaaaaaa gactaaagga tgttaagaat gcttatgtta taggtacaga
     2341 ggatacaata ggtaaatctg tagaaaatca acttaagaat aaagggattg aagttaaaag
     2401 aattggtggg gaagatagaa taaagactag ctatcttata gctaaggaga tttcagctat
     2461 taaaccagta aatgatggtg ataaggtttt tcttgtaaat ggatacacag gtgaggctga
     2521 tgcaatgagt gtatcttctg tagctgctag agatggagtt cctgttatac ttactgatgg
     2581 aaagagtata ccatttaagg ttgatggcgt tcaatgttat tctcttggct cagaggagat
     2641 aatgagtaat gaacttgtga gtaagactaa ttctgttagg attgctggta aggataggtt
     2701 tgagactaat aagaaggtta tacaacgctt ttataaagga acaaagaagt tttatgtttc
     2761 tcaagggtat aagttagttg atgctgtggc tggttctccg ttggctaagg acaaacctat
     2821 agtacttgtt aatgatggaa gtgataagag cgtgcttaga ggtgctgatg aagttacttc
     2881 tttaggtgga atggataaga aggttgttga ccaatgtatt agttctgcat ctgataaaaa
     2941 tacaatgcct acaatcactg ctaatgatgt agaaatttct gtaggggata agtttgataa
     3001 tagtatgttg aatatagttg ccactgatta ttatggaaat aatttgaagc ctaatattga
     3061 gggaactgtg gataccagta aggcaggaac gtatgttttg aatataagtg cttttgataa
     3121 ttttgggcaa aagtgtgaga ttaatgtaaa tgtcaaagta attataaaca caagtacaaa
     3181 agaccctaat agttatgagt ttaaggctat ggtatctaat gaaatataca atctagttaa
     3241 ttcttataga gatgaaaaag gtaaaaagcc tttgaaagaa ttgaaatctt tgactggaat
     3301 ggcaaatgca tggtctaagt acatgtatga aaaggaaata tttgctcatg aaattaatgg
     3361 gaaaaatgca gctgaggtat tttctggttt tggaatgaga agtggtgaaa atattgcata
     3421 tctgcctatg aatactcaat ctatatatgg agacaaagaa gcaaaagaga ttgctaattc
     3481 tatctttgat ttatggaaaa agtcttcaaa atataatgat aatctattaa aaagtgaatt
     3541 ttacagtttc ggatttggta tgtatgtatc atcaaatagt gaagtacatg ctacaatgga
     3601 atttttaaat agttaattta aaattgaaac gataatttaa aaatgaaaat aattttaaaa
     3661 aataaaattt tagggaaaaa agaagaaaaa agttggaaaa aacttctaaa aattttgtat
     3721 aacttattga aaatttattt tagatagtct atcatagaga tgtgagaaat atttggatta
     3781 atatgaacat gaaattttaa tgtacataat aagagatgta atttaatata atgttgggag
     3841 gaatttttaa atgaaaaaaa gaaatttagc aatggctatg gcagctgtta ctgtagtagg
     3901 ttctgctgct ccagtttttg cagcagcttc agatgtaata tcactacaag atggtacaaa
     3961 tgataagtat acagtatcaa atactaaagc tagtgactta gtaaaggata ttttagcagc
     4021 acaaaactta acaacaggtg cagttatttt gaacaaagat acaaaagtta ctttctatga
     4081 tgcaaatgag aaagattctt caactccaac tggagataaa aaagtttatt cagaacaaac
     4141 tttaactaca gctaatggaa atgaagatta tgtaaagaca actttaaaaa atttagatgc
     4201 aggagaatat gctattatag atttaactta taataatgct aaaactgttg aaattaaagt
     4261 agtagcagct agtgaaaaaa cagtagttgt atctagtgat gcgaaaaata gtgcaaaaga
     4321 tatagctgaa aaatatgtgt ttgaagacaa agacttagaa aatgcactaa aaactataaa
     4381 tgcctcagat ttcagtaaaa ctgatagtta ctatcaagta gttctttatc caaaaggaaa
     4441 gagattacaa ggtttctcaa cttatagagc tacaaattat aatgaaggaa ctgcatatgg
     4501 taatacacca gtaatattaa ctctaaaatc tactagtaag agtaatttaa agactgcagt
     4561 agaagagtta caaaaattga atgctagtta ttctaatact acaactttag ctggtgatga
     4621 cagaatacaa acagctatag agataagtaa agaatattac aataatgatg gcgagaaatc
     4681 agatcattca gctgatgtta aagagaatgt taaaaatgtt gtattagtag gtgcaaatgc
     4741 actagtagat ggattagttg cggctccttt agcagcagaa aaagatgctc cactattatt
     4801 aacttcaaaa gataaattag attcgtcagt aaaatctgaa ataaagagag ttttagactt
     4861 aaaaacttca acagaagtaa caggaaaaac agtttatata gctggtggag ttaatagtgt
     4921 atctaaagaa gttgtaacag aattagaatc aatgggatta aaagttgaaa gattctcagg
     4981 tgatgataga tatgaaactt ctttaaaaat agcagatgaa ataggcttag ataatgataa
     5041 ggcttatgta gttggtggaa caggattagc agatgccatg agtatagctt cagttgcttc
     5101 tactaaatta gatggtaatg gtgttgtaga tagaacaaat ggacatgcta ctccaatagt
     5161 tgttgtagat ggaaaagctg ataaaatatc tgatgactta gatagtttct taggaagcgc
     5221 tgatgtagat ataataggtg gatttgcaag tgtatctgaa aagatggaag aagctatatc
     5281 agatgctact ggtaaaggcg ttacaagagt taaaggcgac gatagacaag acactaactc
     5341 tgaagttata aaaacatatt atgctaatga tactgaaata gctaaagctg cagttttaga
     5401 taaagattca ggtgcttcaa gtagtgatgc aggagtattt aatttctatg tagctaaaga
     5461 tggatctaca aaagaagatc aattagttga tgcattagca gtaggagctg ttgctggata
     5521 taaacttgct ccagttgtat tagctactga ttctttatct tctgatcaat cggttgctat
     5581 aagcaaagtt gtaggagaaa aatattctaa agatttaaca caagttggtc aaggaatagc
     5641 taattcagtt ataaacaaaa taaaagattt attagatatg taataaattt tcttgataaa
     5701 ataaagaact ccttataaag gagttctttt attgtgttaa atattcaaaa aagcaaaaag
     5761 gagactagaa tagtctcctt ttattgtgag aaatacttaa atataagaga tataactatt
     5821 tatttaagta aacttattat actatactta aaaaaaaatg tctacttatc cataatttta
     5881 atgtataata gtatttgaga aacaaataag gaggaaaact acaaatgaca gttttagata
     5941 gaattgtaga taagtcagat gaattagaaa ttaaagtact taattatata gttgatgata
     6001 tagagacttt ggaaccaaaa atggaatctt taagtgatga agaactaaaa gatatgacaa
     6061 atatatttaa agatagatta aaacaaggtg aaactttaga tgacatattg caagaagctt
     6121 ttgcagtggt tcgagaagtt tctaaaagag tacttggaat gcgtcaatac agagttcaat
     6181 taattggggg tatagtacta catcaaggaa aaatagcaga aatgagaact ggtgaaggta
     6241 aaacactagt tggtgttgct cctatatatt taaatgctct cttagggaaa ggcgttcatg
     6301 tagttactgt caacgaatat cttgcacaaa gagataaaga aactatgcaa ccagtttatg
     6361 aattcttagg gttaagtgta ggagttatta cgtctgattt attgccagaa gatagaaaaa
     6421 aacaatataa ttgtgatata acttatggta caaacaatga gtttggattt gattatttaa
     6481 gagataatat ggtaactaga aaatctgaaa aagttcaaag agagttatat tattctatag
     6541 tggatgaagt agattcaatt ttgatagatg aagcaagaac tccgcttata attgctggta
     6601 gtggaaaaga agacactaag ttgtatgaac ttgcaaatgc ttttattaaa actttaaaag
     6661 ataaagatta tgagatagat aagaaagaaa acactgtatg tctcacacac acaggtctaa
     6721 gtaaagcaga gtcttttttt ggtatagaaa atataactaa tatagataat atagagattt
     6781 accatcatac aaaccaagca ttaagagcac ataaactaat ggagagagat gttgagtatg
     6841 ttgttaagga tgaagaaata caaatagtag atgaatttac tggtaggata ttagatggaa
     6901 gaagatatac agatggtctt caccaagcta tagaagcaaa agaaggggta aagattaaag
     6961 atgagtctaa aacattagca acagttactt ttcaaaattt ctttagatta tatgaaaagc
     7021 tttcaggaat gacaggtact gcaaaaacag aagaaagcga atttgaatca atttataagt
     7081 taaatgttgt tcagatacca acaaataaac ctgttataag agctgattta caagataaag
     7141 tatttaggac agaagatgca aagtataatg cagttgtaga agaagtaata aagataaata
     7201 aaactaagca accagtatta gtgggtactg tttcaataga aaaatcagaa atgctttcaa
     7261 aactattaaa aaagcgagga ataaagcatg aagttctaaa tgcaaaacaa catgataaag
     7321 aagctgaaat tatatcaagg gctggaaaat taggttctgt tactattgca acgaatatgg
     7381 caggtagagg aactgatata gctttaggcg caggagataa aattgaagaa aaaaaagtaa
     7441 gagatattgg tggattatat gttataggta ctgaaagaca tgaatcaaga agaatagata
     7501 atcaacttag aggtcgttct ggacgtcaag gagacccagg gacatctaga ttctttgtta
     7561 gtttagaaga tgaaatgata aagttatatg gtggaaaagg aattgaaaag attttaaaaa
     7621 ggatgaatgc taaagatgaa gatgcaattg agagtaaatc attatctaaa gccatagaaa
     7681 gagctcaaag aggaatagaa ggtaagaatt ttgagcaaag aaaaaatgta cttaaatatg
     7741 atgatactat taatgagcaa agaaaagtta tatatgagga aagaaataga gttttaaatg
     7801 atttaaatat atctgaagat atacaaaaaa tggttagaga aataatagaa gatgcagcta
     7861 ataaatattt atctaatcaa agagattatt atggttactt taaatctctg tacaatgatt
     7921 ttatgcctcc aaatacattg ttgattccag gggtagataa aaaaagtgta gatgaagttg
     7981 tacaacacac atatgaaata tccaaaagag tttatgatat taaaaaaatg gaagttggta
     8041 tagacaaaat agaagaatta gaaaagagag ttttattgca agtagttgat acttattgga
     8101 tggaccatat agatgccatg gatcaactaa gacaatatat aggtcttaag tcatatgcac
     8161 aaaaagatcc atttaaggag tatgctttag aaggatatga tatgtttgaa gaactaaata
     8221 gaaaaataag aacaacaaca gttcaatatt tatacaagtt tgattaatta agaacacaat
     8281 tcaaaatagt ttttagaatt attttgaatt gtatcaagta ataatatagg atctaattat
     8341 ttaacttaga aaaaagaagc tatatttaaa ataataatta ttttaaaata tagcttcttt
     8401 ttgtgtgaga aatacggaaa aaagagatga ttgaaatttc tttcttcctt ttaatattat
     8461 actataaaga gtattaaaag tctattagtt ataagtggta aaattaaata aaattacaaa
     8521 attgttactt ttatgtatac ataaatatat gccaatattt tacatggaaa aaaatgatta
     8581 atttttgtat aatgaaataa agattctaat
//
LOCUS       DAMJYC010000138         8543 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJYC010000138 DAMJYC010000000
VERSION     DAMJYC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.144"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            211..1197
                     /locus_tag="PC472_003576"
     CDS             211..1197
                     /locus_tag="PC472_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2728873.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1366..1514
                     /locus_tag="PC472_003577"
                     /pseudo
     CDS             1366..1514
                     /locus_tag="PC472_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1533..3014
                     /locus_tag="PC472_003578"
     CDS             1533..3014
                     /locus_tag="PC472_003578"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2728874.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLSYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3040..4011
                     /locus_tag="PC472_003579"
     CDS             3040..4011
                     /locus_tag="PC472_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2728875.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4267..4869)
                     /locus_tag="PC472_003580"
     CDS             complement(4267..4869)
                     /locus_tag="PC472_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2728876.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5150..6361
                     /locus_tag="PC472_003581"
     CDS             5150..6361
                     /locus_tag="PC472_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2728877.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6429..6626
                     /locus_tag="PC472_003582"
     CDS             6429..6626
                     /locus_tag="PC472_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2728878.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6660..7631
                     /locus_tag="PC472_003583"
     CDS             6660..7631
                     /locus_tag="PC472_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2728879.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7818..8195
                     /locus_tag="PC472_003584"
     CDS             7818..8195
                     /locus_tag="PC472_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2728880.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3473 a          897 c         1361 g         2812 t
ORIGIN      
        1 atcaattcat agtagataaa attgaaatta aatataacaa caaaaaatga aaattacaat
       61 taatttaatg ttaaaggctc aattctttgg atgtggatat ggatagtaca agtagtatat
      121 aaaaagtatt gactggacaa ataactatat tttattttaa atatataata taatgtaata
      181 aaatatatca aatgtagaag gtgataacct atgaaaaaaa taacaataaa tgatattgct
      241 aacttggcag gagtatctaa aagtactgta tcaagatatc taaataataa agatatcagt
      301 gattctacta aagagaaaat aaagacaata atagatgaat atggatatga gccgaatgca
      361 tttgctcaaa gtttgagagc taaaaaaaca tactttattg gtattataac accgtgttta
      421 gattcttttg taaaatctaa aattatgatg gcaatagatg aagaactcaa agaattaaaa
      481 tatacatcac taattattaa tactaataga aaaataaggt cagaaataga tagtatatca
      541 aagttagcta gtctaaaagt tgatggaata atactaattg gtaccgaaat aactaaagaa
      601 cataagaatg aaattgaaaa gttagatata ccgatagttg tagttggaca aaaagttgat
      661 ggtatcaata gcatagtgaa tgatgattat ggagcaggat ataaaatggg tcaatatatt
      721 gctaataaag gttacaaaaa cattgtatat ttaggtgttg atgaaagtga tatatcagtt
      781 ggtttaaata gaaaaaatgg agtacttaat gggttaaaag ataaagggta tgatgcaaaa
      841 gtattttata ctgactttga ccaagaaacg tctatacaaa gaagtggaga aatgctagaa
      901 agtgaaaatc cagatataat aatatgtgca actgataata tagcaatagc aacaatgaaa
      961 gaaatcaata agagaggtaa aagtatacca caagatatat ctgtagctgg atttggggga
     1021 tatgatgtat tgtctattat aagccctaaa cttacaacaa taaagtttga aaataaaaat
     1081 gcaggtaaag tagcagcaaa tacgatagta aatttaatac aagaaagaaa agaaccacta
     1141 ttaaaggaga ttaaatttga attaatagaa ggtgaaagta caataaataa aaattaagat
     1201 acacattaca tatattagta atgtgtattt ttttactaaa aaaactataa ttctaaaaat
     1261 taggaaaact ttttcgaaag aaatattgca tctgaaataa atttatgcta atctttaatt
     1321 aaaccggttt cgaaaccggt ttaattaaac cgatagaata gcaaaatgag tgcaaaaata
     1381 actgattgta acaaagtatc aaagtaatta ttagagtaca taggaataaa caaaatataa
     1441 aagatgtagc acattgtgta ataagactaa gaattgtttt agatagcaat aataaagcaa
     1501 atataaaatt ataaaagaaa aggaataaaa atatgtacaa agagcctaaa tatagaacca
     1561 tattagaatc gacaaaagaa gaattaaata aattaagaaa tatatcttta aatgataaat
     1621 ataaaccact atttcatata catccacaac atggactact taatgaccca aatggattat
     1681 catattataa tggtaagtac catgtatttt atcaatggta tccatatgat gcaactcatg
     1741 gaatgaagca ttgggcttat gtaagttctg atgattttgt aaactggaat agagaagatg
     1801 tagcattaat accaatcgaa agttatgaat ctcatggagc atattcaggt aatgcgatag
     1861 aggtagatgg aaaattgcat atgtattata cagggaatat aaaatatagt gcagaagata
     1921 gatatgcata tcaaaactta gctataatga acaaggatgg taaaataact aaatatgaaa
     1981 ataatccaat agtaagtgaa ataccaaaag gatatacagg acatgtaaga gacccaaaag
     2041 tatttaaaag aaaggataaa tactttatgt tacttggagc acaaacaagt gataaaaaag
     2101 gagttataat agtatatgaa tctaaaaact ctatagattg gaattttaaa ggagaattaa
     2161 atgtaaaaaa catagatgaa gattttggtt acatgtggga atgtccagat tatattaata
     2221 tagatgaaaa agatatatta atattttcac cacaaggagt agaacctaaa ggatttgatt
     2281 atcaaaatat atataatgta gtatatgcta ttggtaatat ggatttagat aatttaacat
     2341 ttgaaataga tacaatgaaa gaactggaaa aaggatttga tttctatgct ccacaaacat
     2401 ttataaagga ctcacaaata atactatttg catgggctgg aatgggagaa gttttatacc
     2461 ctacagataa aaacaaatgg gcacattgtc taactgttcc aagaaaatta aatataaaaa
     2521 ataataagct attgcaaatg cctgtagatg aattaattaa attaagatat gatgaaacaa
     2581 gtgggcaaaa tactataaag aataatatca atattataga aaataatgaa aatgtatatg
     2641 agcttaatat taatataaaa aatattgaca gcaataaatt tgggctggaa ttattttcat
     2701 cacaagatga aggtgtaaaa ttagaattta acaaattagg aaacattgta actttagaca
     2761 gaagcaactt taaaaaagtt ttcggtgttg aatatggaac aaatagaaaa gaatatataa
     2821 atattgatga aaatactaat atcaaagtac tagcggatag aagtatttta gaaattttta
     2881 taaatgaagg agaagtagta tttactagta gaatatttgc taaagaaaat tctaatcaaa
     2941 taagggttta tagtgataaa atagtagagt atgagtatac taaatttaaa ttaaagcaag
     3001 gtattgaatt ataaaaacac attataaggg gtagaaaaga tgaaaaaagt aattagcata
     3061 ggagaagctt taatagactt tataccgaat caaaatggtg gtaaattaaa aaatgtatca
     3121 gaatttagaa gagtagcagg aggagctcca gcaaatgtta gtgctgttgt agcaaagctt
     3181 ggaggaaaat cgagttttat atctaagtta ggaaaagatg catttggtga ttatataata
     3241 gatgtattaa atgaagtaaa tgtaaatact gattatgtat tgagaacaag taaagcaaat
     3301 acaggacttg cttttgtatc attaaaagaa gatggtaatc gtgatttctc attttataga
     3361 aatccaagtg cagatatgct tttagaagct gatgaagtta aaaaagagtg gtttaataat
     3421 tgtcatatcc tacatttttg ttcagtagat ttaatagata gtccaatgaa attagcacat
     3481 aaaaaagcaa tagaatacgc acttgaaagt aatagtataa taagctttga ccctaatata
     3541 agattacctt tatgggatag tgagcaatct tgtaaaaagg caatatcaga atttttacct
     3601 tttgctcata ttgttaagat atctgatgaa gaattagaat ttataacagg agaaaatgat
     3661 atagaaaaat cattacataa actctttgtg gggaatgttg aactagtatt atatacaaaa
     3721 ggaaaagatg gagtagatgc atacactaaa aaagtaaaag gtatgtgtaa aggggttaaa
     3781 gtaaatgcaa tagatacaac cggagcagga gattcttata taggttcatt tttatacacc
     3841 ttattgtata aacaaataac attgaatgat ataaaagaaa tgaaacaaga aacgttaaat
     3901 gagtatttag aattttctaa ttattatgca gctcgtagta caactattaa aggtgcgata
     3961 agttcatatg caacaaagga agaaataact gaatttataa aaaatttata gtaggaatga
     4021 gtaataggtt ttgaaggaag taacatgtaa agagttttta aggatgttta ctatgaaaat
     4081 aattaacata taaaaagcaa taaagaggct gtctcaaaat agagataagc ctctttatca
     4141 ttgttgaaat gataaaaaat attttaatta ttgttacttt ttagaagaat atatatctta
     4201 ttttaaataa gcaaattatg taagatttat ttatattatc aatactttac ttggagtatt
     4261 atgtctttag cagttatcta cttcaattga attatctaaa atataataaa tttgttttgc
     4321 aaattgacga gaatgttctt ctttttcttt attcataaaa ttaggtttac ctaaatcacg
     4381 ttcaacaagc attcctatat attttaactg tgaatgctta cagtagcgct tcataccatt
     4441 ttcccaaata tcagcaccct tttcaggtct atatccacat gttgtaataa gtgctactgg
     4501 ttttcctgcc catagagcgg ggcctttctc attaccatag tatttattca ttccatacac
     4561 caatctgtca agtagtgctt tcattggaga agtgcagtac caagaataaa ttggagtagc
     4621 aaaaacaatt aaatcacaca taagaatttt cttgaaaatt ttctgaacat catctttaca
     4681 ataacatcca aaatcaaata agttatcctg acatttttta caagcaatac atggctctat
     4741 tttacaatca tataaccata ttacttcaat atttgaatta taattttcta attcctcaat
     4801 aaaaggtttt aaaatagcag cagtattacc ttttttctta ggacttccca taagaatgca
     4861 tacttccata ttgatacctc cacagtattt taaaatagtt tttagtgctt aatatagttt
     4921 taggatacta tgagaatacg ttaacaacaa tacactttgt atatttgtaa taaaattgaa
     4981 acaaattgtt ttatcaataa aagttggaat gtatctataa ttaaaaatat tttaggtatg
     5041 tttttacata tatgtgtgta gttaactatg gtaatttatt gtaaaattat cttataggat
     5101 tataattgct taatttatga ttatatttaa aatcttggag gtaaaaaata tgtactcaaa
     5161 tgaagactat aaatttatag aagaaaatgc accatgttat atatatgatg agactaatat
     5221 aattgaaaaa tgtaaggaat tgaaaaataa tattttaggt tttgattttt tatattctat
     5281 aaaaacaaat ccatataaca aaattgtaaa aagcattgca gagcaaggtt ttggagcaga
     5341 tgcttcttca actaatgaag ttatgatttc atcagaatgt ggagtttcta aagatgatat
     5401 ttattattca tcaccaggca aaacagagat tgatatagaa actgttttag ataaatgcat
     5461 aattattgca gatagtatta cagaaataga acttataaat aagatatcta aaaagcataa
     5521 tcagaaagtt aaaataggaa taagaataaa tcctgatttt actatggatg atgatagtgc
     5581 taaatcaagc aaatttggta tagatataga agatatgagc agtgttttta agatgttaag
     5641 tatgtgtgat tatgtatctg tagttggtat tcatatacat attaaatctc aggtattgga
     5701 ttttgaaaag ttaggacatt attatgaaaa atgttttttt atagctttaa atttaaataa
     5761 aagtaagtat attaatattg aatttataaa ttttggtagt ggacttggag tagcttatga
     5821 taatcttaga gaaaatccac ttaatcttaa agagctttca aaattaactc aaaagattgc
     5881 ttttactaat tcaaacacac taaaatcaag acttcttatt gaatcaggac gtttcatagt
     5941 ttgtaaatca ggtaattatt atgttaaaat tgtagatatt aaagtatcaa gagggaaaaa
     6001 atatcttatt gtaaaaaatg gacttaatgg atttatgaga cctattattt ctaatttaat
     6061 tgaaaatatt acttgtggag tagaattaga aggtcaagag ccattatata catcaaatca
     6121 tgccttttct ctttcagtat taaataattt aaaagaagaa gagtatgtaa atgttgttgg
     6181 gaatctctgt acatcattag atgtaattgc aaataatgtt aaattgaaaa aagcaaacat
     6241 aggagatttg atttcaatta gtaatgctgg aagttactcc tattctttat caccgctatt
     6301 attttcaagt tatgaatttc ctaaacaata ttataaaaga aaagatggat atattgagta
     6361 aacaatagca taaattaata aacttagtat tattgaaaaa atttaaaatg cagggggtgt
     6421 aattctatat ggaattgtgg aaaagaaatt tatttgtatg ttggataggt atgtttttta
     6481 gttctatagg gatgagccaa atagcaccta tacttccatt atatattaaa cagttggggg
     6541 taactgatgt tagtttaata caacagtatt caggtataat ttttggatgt acctttgtag
     6601 tagcagcatt cttttcgcca atttgaggga aagctgctga taaatatgga agaaaatcaa
     6661 tgcttttacg tgcaagtatt gggatgggaa tagttatatt taccatggcg tttgttcaaa
     6721 atgtctatca gttattagga ttaagaatac ttcaaggagt ttttacgggt tatgctactg
     6781 catgtactac tcttattgca acacagacag ataaaaatca ttcaggatgg gctttgggta
     6841 ctcttgcaac tgcatcaact actggctcat taattggtcc aacagtagga ggatatatag
     6901 aatcaatctt aggtcttaaa agtacattta ttataacagg aggattgttg tttgtatcat
     6961 ttatcatctc tattttattt gtagtagata attttaaacc taaagaaata aaagaatcaa
     7021 tttctatacc taaagaacaa ttaaatatat tgccaaataa atttttaatt gcatctattt
     7081 ttgtaaccac attcattact caactagctt tatactctat agaaccaatt gtaaccatat
     7141 atatatcaca acttacaaat tttgcatcaa atgtagcttt aatagctgga ttgacatttt
     7201 ctgcatcagg attagcaaat ttattagcag ctcaaaaact tggaaaaatg tcagataaaa
     7261 taggtcctca aaaagtattg ttaatttcac ttttatgggc tgggattata tttataccac
     7321 aagcttttgt aaaaactgca tggcaactga tgttgttgag atttttatta gggttgtcag
     7381 ttgcagggct taatccttct gttaacagct tgcttaaaaa aattgcacca gaagaatatg
     7441 tgggaaaaat atttggatat aatgcatctg cccagtatat aggctgttca tcaggagcat
     7501 ttttaggggg acaaatatca gcacatttag gtattagaac agtatttttt tcaacaagtt
     7561 tattgttgtt tatgaatgca ctttggatgt ataaatttac tggattgttt attaaagata
     7621 agactaaata atcatactat ttacatttct aattttatga tgtaaaataa atatttttta
     7681 tgtaatttat catagaatta tataaaaaat aaccctatgg taaaaattgg aaaatattat
     7741 tatcgacaaa atctttgact taaaaactat attgcgatag tattaattta ataaaagttt
     7801 gagaggggcg taaggttatg acaatttcaa aaataccaca agcagaatta aaagttatga
     7861 agtttatttg ggaaaaaaat gatacagtaa catcaaaaga aactattgaa gctatggaac
     7921 gaaaatatgg ttggaaacaa acaactacat tgacactttt atcaagactt gttaaaaaac
     7981 attttttaag tgcagaaaaa atagatagat acactcatta tacaatagtt gttggacata
     8041 aggagtattt aagtgttgag acaaaagatt ttttaagcaa tatacacgac aattctttac
     8101 agagtttaat ttcagcacta catgatgatg aagttgttga taaagatatg ttggatttat
     8161 ttgaaagtca ttttaaaaat ttagaagaag agtaattata tcaggataga actctaagaa
     8221 tttaattaat ctatggctga aaaccataaa tggtattatt taatcagcta ctcactattc
     8281 ataaagtaat attaaatagg ttctctaatt agtaaaatgc ctaaaataat tggaataggg
     8341 agtatcaagt taaatgatac cacttattta aataagaaat gatttttaaa atttaaaata
     8401 attttataac ttgtcaatat tcattaatct aaagtattat ttctatttaa ccatatcaaa
     8461 atatatttta ctatttttaa ttattacaat ataaacctgt cttttgttat tataggtaaa
     8521 ctaaaagtaa gaaatgattt tta
//
LOCUS       DAMJYC010000139         8362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJYC010000139 DAMJYC010000000
VERSION     DAMJYC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.145"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(170..1039)
                     /locus_tag="PC472_003585"
     CDS             complement(170..1039)
                     /locus_tag="PC472_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2728881.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1136..1993)
                     /locus_tag="PC472_003586"
     CDS             complement(1136..1993)
                     /locus_tag="PC472_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2728882.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2208..4226)
                     /locus_tag="PC472_003587"
     CDS             complement(2208..4226)
                     /locus_tag="PC472_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2728883.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4497..5273)
                     /locus_tag="PC472_003588"
     CDS             complement(4497..5273)
                     /locus_tag="PC472_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2728884.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5330..6301)
                     /gene="asrC"
                     /locus_tag="PC472_003589"
     CDS             complement(5330..6301)
                     /gene="asrC"
                     /locus_tag="PC472_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2728885.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6314..7105)
                     /gene="asrB"
                     /locus_tag="PC472_003590"
     CDS             complement(6314..7105)
                     /gene="asrB"
                     /locus_tag="PC472_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2728886.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7106..8113)
                     /gene="asrA"
                     /locus_tag="PC472_003591"
     CDS             complement(7106..8113)
                     /gene="asrA"
                     /locus_tag="PC472_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2728887.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2690 a         1511 c          758 g         3403 t
ORIGIN      
        1 actagttcac attttcatta ttattgatat attatatatg aatataccat tttgtttttt
       61 aataaaaaat catgctacat caatagtaag caactattaa tgtagcatga ttttttgtat
      121 ctaaataatc ttagtattta cctatcagta tagattgata tatcaaaaac tatagtcttc
      181 tatattcttc tctttttagc ttttggttac ttagatagta atcaatttca tcaattagac
      241 gttgtttatc atcaggtaat acacctttta aattagcata atttgatgca tgattacttc
      301 taaatacaat tttttcatta gcatctatat tctttatcat ctctttaatt tcttgtaaca
      361 cagccttatc ctctagtatt gtaaactctc ctgccttaag taaatcgtaa agttcagtat
      421 taggttctac cattaaacta agtacaccca cataatctgg tgaaattgct cctagcattt
      481 ttcctgtatc aactgcatgc tgatgtgttt ttttagttcc tccaagacct gctattacag
      541 tggctgataa tttaatccca ctttttttaa cttttctacc aacttttatt aattgttcac
      601 tactaaaacc tttcttaata tcttctaata cttcattgct tccactttca agccctagat
      661 aaatcatttt aacaccaagt tttcttaatt ctttaagttc atcatcagat tttttctcta
      721 tagatttagg agagccgtac atagtaattc tttcacactc tggaaaaact tcttttatat
      781 ataaaataat atctcttaat ttctccattg gtattatcaa tgcatctcca tctgctaaga
      841 atattctttc tacatgctta taatatcttc taaatatatc aatttcattt tttattactt
      901 ctagtggctt tattttaaat tttttagatt tgtacatact acaaaacgta catttgttat
      961 gagaacatcc tagtgtgact tgtattataa gactataagc ctcacttggt ggtctgtaaa
     1021 gtggcatatc gtattccata ttaaaatccc ccttgtaata aaatattcat tctattatat
     1081 tattaatatt tactttaaat aataacttat aaaattatat tttttaaatt ttagtttaag
     1141 atatattctt taaaaattta atgatactat tggcagtaat attaagttgt ttttcttgac
     1201 ttataatagc atcattatct cccttttgtt ttccataatc tccaaattgt gcatgatttc
     1261 ctccttcaat ctcaacataa gttgtatcat caggaagttt ttgttttgat tctacaagac
     1321 ttttaaaatt gactactcca tctttgctcc cccatataga tataactttt tttcctaatt
     1381 ctttcaactc atcacccatt ggataagaag ctaaaaatac aacgccttca attaatttat
     1441 tatcacgtgc aaaatttgat gctgcaactc ctcctaatga atgacctcct attacccaat
     1501 gttctatgtt gttgtaagaa tccataactt tctgtgcctt attagttcca agtatagcta
     1561 gattaagtgg catctttacg attacaactt catatccact ttctgctatt tttcttgcaa
     1621 gtggagcata agattctatt tctactttag ctcctggata caaaattaaa cctttagaaa
     1681 cttttcttcc ttttggtgta aatgaaatgt tttcatttac agttacttca actttactat
     1741 tgctgactag agcttctttt gccagctttt gaggttcata tgttttattt atccatatga
     1801 aaaagccaag caaaatcaat acaaatagta tacctattcc cttaaatatc ttattcttta
     1861 tgttaaattt tctattatta tttttatcat ctttgttctc aacctcatca taaagtaaag
     1921 gtctctcaag ttccatagtt atactaaatg tatcatattt cttattacgc tttaccttta
     1981 catctttact cataaatcta acccccataa tatttgaata ctattatagt ttatcctaag
     2041 taaattgttt tttcaaccaa tgaaaaaaat caatatttta actattttca tataaaattc
     2101 ataaatatat gttttttgtt ttttatatat tattttcttt tagtatttta taaaacaaat
     2161 agccatatac tttattttaa atatatggct attctataat ttaatatcta agctgcttct
     2221 tttattgaaa ctttagttat attacttatt ctatacattt ctttgacacc atttagagtt
     2281 tgcagtaagt ttagacatat tatcttgtca cctagacttt tagtatttat aatactctct
     2341 aaaattacac ttcttccttc atcatactta tctacattgt aatttgaaat ataattagta
     2401 tctctcaata ttttttcaac atcttcatct tttattttat ttttattaac atttatatat
     2461 agattataca tattacttat tagtaatttc tgattttcta aaaaatcctc ttgatgttta
     2521 tctttatata tagcatttat caacataagt ctatcttcaa taagtgatgg tatcaataat
     2581 aaatttttta tatagtaaac atcagctaca ttttctatag acttattgac ttcatcaatt
     2641 atatttttaa ctattccttt atacatttct ataacatgtt gatagcctgt tttagcatca
     2701 tatggtaata taaacttatt tactattaat gcaataattg ctcctaatgc tacatatgaa
     2761 atcctgctta tggttaaaac atttgggtct cccataacta ctgctgaacc aagagctgaa
     2821 acagttacac ataccattaa ttttctataa tcaactacat aagagtttat ataccctgct
     2881 aaaagaacta ttaacgacct taatgtacta tcttttatta ttgaaaataa tacaataaat
     2941 attacagctc ctataaatgt accttctatt ctttttctag acctttgtat acaattttct
     3001 gcgtaaggtt gtgttaatga aaagacagtt aacattatcc atcttccatc tcttaaatgg
     3061 aaataatcca taataaaacc cgctacagct gtagctaaag caatctttac tgcataagaa
     3121 aatcttatag aatttaaatt aaagtttttc ttatatattg atagtcgttt aaattcatga
     3181 ggtatctttg attttctttc agctttcttt tcgtctcttg attttacttt tttataatct
     3241 actaaaaact catataaatt ttctaaaaca ctataaattt cttttaaatt tatattttta
     3301 gtatcctgag caataacata atctctaaat cctaatatct ctttatcagt tatggtatct
     3361 ttattttcta aacacatctt aatatagttt atttctttcg ttgctacaac tagaacatct
     3421 gatttactat ttatgctact ttcttctaaa atatttttta tatcttcatc ttcaaattct
     3481 ttactatcct ttttgtatct ttctaggatt aaacttattc tttctaatga aaataaaata
     3541 ttcattatgt ctatacctct aatactgata taaaagtcat cttttctatt atcaaaaata
     3601 accattttca aatttttaga tatagaattt attctataat tacagctatc tatttttctc
     3661 atactagatt taattataga actatcatca ttaatagtat tttttaaaag actaattttt
     3721 tctaataatt catcacatat agatatcagt tctttatctc cgacattttt catcctattt
     3781 ctattcataa caaattgagc aatcattatt ataactgctc caacagctaa agccgacaat
     3841 ctcataggaa attcactttt tcctacaggt atacttacca taaatatata cattagacca
     3901 aagggtacat atactgctct ttttaagtca taactaaata cataccctat tataaataat
     3961 gctataaaat ttaataaaac tcctaaatac atattttgta ctgcaaaaaa tgatagtatt
     4021 cctaagatta tatttattgc taaatacttc agtaaatttt taattggaga aatagttaaa
     4081 tctctttcca agagcgttag catagctgtt actataccta cacctataag tgtattttta
     4141 cttccaaata aatatataaa tatagaaata aatgctatta taaaaataaa caactttgtt
     4201 tttgatatta ctagtttctt tgtcatcata tcccccctaa aaatttcaac aagaccaatt
     4261 ttatcatatt tgttatctta ttcaaaatgc gcttttttac ggtaggactt taactttttt
     4321 tatatttaga actatataat tcatatataa acgtattttt tatatatata accgatatat
     4381 tttcgtatgc tcccatattt tttttgtttc aaatattttt tcaaaaatat aaaaataact
     4441 ctctcaacaa tactaaaaat gcatttatca agagagttat aatttataca ataaatttaa
     4501 ttaagctttt ctttacctaa tatataagtt ccaagaccca taaataaagc tcctccaaca
     4561 atatttccaa gtgttacagc tactaaatta taaatagctc ctccaatagt aactgtattt
     4621 attgttggtg aaaataatga aacactataa attgtcatat ttgctacact atgctcaaag
     4681 cctgatgtta taaaagcaaa taaacataag aatatcataa ttatttttgc agtatcacta
     4741 ttagttctaa acgaacacaa cactgataca catactagaa tattacataa aatccctcta
     4801 aagaaaagag ctgtaaatgg catggatgct ttactagctg ctgtatttgc aaaaaattca
     4861 gctacaggtc ctttgtctat tagaccagtt cctacaaata taatgcctaa aaccaaagct
     4921 ccgattaaat tacctaccca actgtatgcc catattttag aagtatcttt tatacttaca
     4981 cctttattta acataccagc agacataacc atattgtttc cagtaaataa ctctgttccc
     5041 gtcattataa ccaaacttag tgctattgca aatgataacc ccattactat cttagtcata
     5101 ggtgaacctg catcagtcaa aagtcctcca atagtaaata tcaaaagtat acctatacct
     5161 acataaagtc ctgcaaacgc agaagatacc aaatatttta ctttacttgt gtttaataaa
     5221 tttatcttat ttatagctgc attagttaat ttatccaaag tttctttatg cataattccc
     5281 tcttccttct attaaatttt aatttattca aatatatgtt ttactcaatt tatagatatt
     5341 taattccact ccagtatatg ttttcgtaca ttttagttat ttctggaagc tctacacctt
     5401 caagtgccca ttttttaaat tccataaatc ctgttctatc aattatatat cctatatgtt
     5461 cttttcctcc tggtgcatct ttatctatat acttttctac atatctataa gtattaagta
     5521 ttatctttat tatgctgtcc tcatctaccc ataaaagaaa atcttctgct agtctagggt
     5581 ttttctttcc tgttctaccc attatagcta atctatagta ttttttctca tctctagtcc
     5641 atgcatttgt tgggcaattt agcacacact ctccacagcc tatacactta tctttatctc
     5701 ttaccacctt attattttct gtttttaatg ctccaacaga tatcttagaa cattttttta
     5761 cacatgctcc acaatttaca catctatcct tttcatatat tggtaaagcc ataccaataa
     5821 ttccaaagtc attcattcta gcttttatac aatcatttgg acaaccagtt aaagccactt
     5881 taaaatgtaa atcatttgga aatatagctt tttctattct tttagcaaat tcagttgtat
     5941 tatattgagc ctttggacag actttatttc ctatacaagc tgctatattt cttgtacctg
     6001 ctgctggata acctgcgttt ttctcacttt gatttatatt cattttttca ataacaggtt
     6061 gtataatttt atttatttct tccatatctt ccatatttat tcctaatatt tcaaacccct
     6121 gtcttgtagt tatgtgcacc tgtccattgc cgtactcact tgcaatttta gaaactacag
     6181 ctaaactctc agggtcaata agacctccag gtactctaac tcttaaagaa gtctcatatt
     6241 ttgtttttgt aattctataa gcatttttca ttactttttt ggtatttaaa tctcttatca
     6301 caaacttcac cccctaatca ataagatttt tagcatatga ataatcaaat actggtccat
     6361 ctaaacatat gtaagtatca tccattttac aatgtccaca tttacctatt ccacaacaca
     6421 tttttctttc ataagaaacc caaatgttct tttcatctaa atctcttttt aaaaattctt
     6481 ctactgtaaa tttcatcatc attggaggtc caactacaac tatagaagta ttgtttatat
     6541 ctttcatatc caattctggt atgtattttg ttactaatcc tacattacca ctatatcctt
     6601 cctctgctcc atctacagta actaaaatat caagtttttt actccatctc tttaaatcat
     6661 ctgcaaataa aatatctttt ggtgatttaa atcctactat taatttaaac ttttcgcatt
     6721 tttctttatt attataaaaa tattcaacta ttcctcttac aggggctaat gcacttcctc
     6781 ctgccactac tacaatttct cttccttcat aaagggatac atcaaatcca tttccataag
     6841 gacctcttat aaaaaaagaa tctccttctt tataattaaa caactcgcta gttacttttc
     6901 ctacatttct tattgtaaag tcaatataat cctctccata tcctgatact gatattggac
     6961 tttcaccgta tttaggtata gatatctcat aaaactttcc aggcaataca ccttttgtat
     7021 tgcagtttac tctaaaagtc cattctttat cagtatgttt aactatatct agtatttttg
     7081 cacttactgg tatatatggg ttcatttata cttcctcctt actaattact aaatcattta
     7141 ttttttctat acactttgaa aatgatatgt attgcggaca ggcatcatca catcttccac
     7201 agccaacaca catatgatat ccgaatcttt ttttaaaatc atgtatttta tgcatagttt
     7261 taaatctcat tctttcgcct tgtttttttc taaaagaatt tccacctgct atatctgtat
     7321 atccatctac ttgacaagaa gcccaaaccc ttcttctctc acctacattt tcgttttcct
     7381 tatagaaaat atcctgcatt gtaaaacatg aacatgttgg acatacaaaa ttacatctac
     7441 cacatcctat acatcttgta tcatattcat cccatattga atttcctatc atttcatttg
     7501 aatcaatatt ctctggcaca tctacagata ttaaattatc agttacataa tcaacattaa
     7561 attcagttgc ttctccatta aatacttcaa attcactatc ctttatatct aagtacagtg
     7621 tatctccttc tatatttaat ccaattgaat aattatcagt tttgttagaa tccatgctca
     7681 cacaaaaaca gtttctaaaa ctttccttac accctacaag tgcaaacttt actttatctc
     7741 tgatattttt ataaaaataa tctttttctt caccattatt tagatatatt tcatcaagtc
     7801 tttttatacc atttatgtca caagctctta agaaaattaa aattttcttg ttgtctaaat
     7861 cgctttcctt aaactctttt tcagtaaaat aaaacaatac ttgatttatg ggaagtacaa
     7921 cttcttttgg tgaaaagttt gctttcttag caaattccat ttcttcaaaa gcgtttacct
     7981 ccttgtatct tataacatca gtatctgaaa aagtaccttt aaatgggatt aaaactggtg
     8041 caaatatctt gtagtctttt tttagataat tcagaccttc attgaactta gatgaatcta
     8101 attttagttt catttttatc acctttctta tattttagtt ttacttactt gctatcttgt
     8161 ttaaatctta acactttatt ttctatgctt ccgtgataat tatcacagtt tcaaaaataa
     8221 taaaaaaaat attaatggag tttattaagt tctgttaata tttttttatt atctacatta
     8281 ttcgatttca aatctaatca cttaaataca atcattaagt acaataaaac tactatctac
     8341 tcaatttttt gaataaaaaa ga
//
LOCUS       DAMJYC010000140         8307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJYC010000140 DAMJYC010000000
VERSION     DAMJYC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.146"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            214..1677
                     /locus_tag="PC472_003592"
     CDS             214..1677
                     /locus_tag="PC472_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2728888.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHEGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1733..2869
                     /locus_tag="PC472_003593"
     CDS             1733..2869
                     /locus_tag="PC472_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2728889.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3105..4221
                     /locus_tag="PC472_003594"
                     /pseudo
     CDS             3105..4221
                     /locus_tag="PC472_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4373..5242
                     /locus_tag="PC472_003595"
                     /pseudo
     CDS             4373..5242
                     /locus_tag="PC472_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            5434..6324
                     /locus_tag="PC472_003596"
     CDS             5434..6324
                     /locus_tag="PC472_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2728890.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEVISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            6542..8296
                     /locus_tag="PC472_003597"
     CDS             6542..8296
                     /locus_tag="PC472_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2728891.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVVKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         3302 a          815 c         1517 g         2673 t
ORIGIN      
        1 acacacgtcg ttaaaaattt tataaattta tgtttagata tgattaatac tcttgatttt
       61 agaaataaat atttgtagat tgtagattaa agataatata aataaataga aggtagattt
      121 tttagatata ttaatataaa gattatataa aatattgaaa tatagctgtt taaattattg
      181 tctataaata taaaatgaat ggtggtgagt taagtgaccg atttagttaa caccattaat
      241 ggaattgttt ggtcacccgt attagttgca atgtgtttgt gtataggatt atatttttca
      301 ttgaaattaa aattttttca aataagagat gtaaaagaaa tgtttactct cttgcttgaa
      361 ggagatagtt ctcatgttgg tatatcatca tttcaaggtt ttgctacatc tatggctgga
      421 agaataggga ctggaaatat agctggtgta gctgtagcaa tagcaatggg aggtccagga
      481 gcattagtat ggatgtgtat aatggctaca ttaggttcag caactgcatt tgtagaatct
      541 actttagctc aaatatataa agatgagcat gaagggcagt atagaggagg acctccatat
      601 tattttgaaa aaggtcttgg gtggaaaaag tgttcagttt tattttcaat agttgcaata
      661 ataagttata cattttttat gcctggtaca caatcaaaca ctttatatct agcaatgaat
      721 caagcatttg gattttcaaa aatgacaata gcagtagcat cttctattgc attagcgata
      781 ataatttttg gaggaattaa aaggattggt ttatttgctg aaaaagtagt acctataatg
      841 ggtggagcat accttattat gacattagta ataattataa caaatattag tttagttcca
      901 gaggctattg taacaatgtt taaatctgca tttggatttg gttcagtttt tggaggaatg
      961 ctaggttcag caatatcttg gggagtaaaa agaggtatat actcaaatga agcaggagaa
     1021 ggttctggga cttatggtgc ggcagcagca gaagtaagtc atccagccaa acaaggatta
     1081 gttcaagcat tttctgttta tgtagacaca ttattaatat gtatggctac aggtattatg
     1141 attgtaataa caggaatgta taatgttaca ggtgtttgca cagcactacc aggtgtagaa
     1201 ccgggaccac aatatgtaca gagtgcaata aatacattac atccacaatt aggtgatatt
     1261 tttatagcaa tagttttatt attatttaca tttacaactt tattagcctt ctcatatatg
     1321 atggagacta atgtatctta cttagtaact agaattaaag cagaaaaaca tgaaaagtac
     1381 ataattaatg tctgtagatt tatgcttata gcatgtgcag caatgtgttg ttttatagaa
     1441 ccacttacaa gctgggcatt aggtgatata tgtataggtg tattaacata tataaattta
     1501 attgccatct ttacactaaa aaaaccagct ataaaggcat attatgatta tataagacag
     1561 aagaagatgg gaataaaaag gagtgataga acttttaatc ctatagagtt agatataaga
     1621 aatgctgagt tttgggaaaa tagatactta aaagaggata agcatgagtt aaattaaagt
     1681 tataataaaa tataaaaatt actaaataaa tttagtggga ggtaagattg gtatgaaaga
     1741 aagaataata caagtggctg attcaaagaa agaaaaaatt ctagggctat gtcaatcttt
     1801 atatgatgaa ccagaaattg ctttgcaaga atataaatct gctaaaaaaa tatcagaatt
     1861 tctaagagaa gagggttttg atgttgagga aaacttggca ggtatggcta cagcttttaa
     1921 agcaaccaag aaaaatggag atggtcctaa aatagcattt atagcagaat atgatgctct
     1981 tccaggcaat ggacatgcat gtggacatca tttaattgca tctatgggag ttggagctgg
     2041 aatagctctt tcaagtatac tggatactta caagggagaa gtaagtataa ttggaacacc
     2101 tgctgaagaa actggagatg gaaagcctta cttgatagaa catggtgtat ttgatggcta
     2161 tgatgcagca atgatgatac atccaaattc aaaaacttgt gtaacacctg aaattatagc
     2221 aataggaggt ctagatttca tatttactgg aaaagcatct catgcaggag ctaagccata
     2281 taacggaata aatgctttag atgcagtagt actactatat aataatataa atgcactaag
     2341 acaacaatta gtagatggaa cgagaataca tggtattata ctagaagcag gaactgctgc
     2401 aaatgtaata cctgatatgg gaaaagtaag attagagata agggcaaaag aacaaaatta
     2461 ctttgatgaa gtagtagaaa aagtgaaaaa ttgtgctaga ggtgctgcaa tagccactgg
     2521 atgtgaactt gaattttatc actttgaacc aacttgccaa ggattaaatg aaaataaagt
     2581 tttggtagac atatttacaa agattatgga ggaatttgga atatatgaag atgaacaagt
     2641 aatgttaggt tcaacagata tgggaaattt aagtcaaata atgccatgta tacatccttt
     2701 gatgaagttt tctgaaaatg gagaagaact acatacaaaa gaatttttgg aggcttctat
     2761 aaattcatat gcaaaagata gagtaattga tggtataaaa atattagctt taacaggatt
     2821 taatttattt gaaaatccag agttattgaa aaaaatgaag gaagagtaga acttagacta
     2881 tttttaatta tactctttaa aataagaaga gtgtctcaaa atgaactttt tagtttatga
     2941 gacactcttt tttgtatgaa attaaatata tttttattat tttaacaatg aaaaagaggc
     3001 ttatctgttt ttatataacc ttttttaact tgtagcacaa aatatcatga atgattacaa
     3061 ttcgagtgat aaaatcgatt ataagatata taggaggggt acatatggaa actttagaag
     3121 taagaaatat tgaattgagt ggtacaaatt atgagatagg atacagacta ggagaattgg
     3181 ttgctaatat gccagagata ctagaaggtc agattaataa atctaatgtg gtaagcaaaa
     3241 aaagaagaaa aagagatgat agagttgttt gataaatatt gtccaggtct taatgaagaa
     3301 ttgcaaggtt ttgctgatgc aattcaagtg aattgtaatc aaattttata ttatactatg
     3361 acttatctta aaccaggttg tagtcaagtt gcattagcac cagaattaac tgaaaatggg
     3421 catgtactat ttgcacgtaa tttcgatttc tcacacaata tggaggattt tgttttatgt
     3481 aaaactaagg taaatggaaa atatgcacat ataggtacta ccgttatgca atttggaaga
     3541 ggtgaaggta tgaatgaatg tggcttaggt gtaagtcagt catcatgtgg tattcctgta
     3601 ggcaattcag atggtttaag aaaaccagca attgtaggtt tacagttttg ggctattatt
     3661 agatatttgt tagaaaattg caaagatgta gatgaagcat tagaatattt aaaggatatg
     3721 ccaattgcat acaatattaa tttattgctt gcagataagt ctggaaacat tgcacttgtg
     3781 gagactttag atgggaaaaa agaagtaaat ataatcaata gtttagaaag taaaagagaa
     3841 tattttttac actctactaa tcatacacat attgataaac ttcataaatt agagccacaa
     3901 tcaatgaaaa attctattca tagatataaa ttaattaaag aatatataaa taagtctaaa
     3961 aaaattggag agaaagagtt aatgaattta ttgtcttcaa aatatccaaa tggcttatct
     4021 tgtaattact ataatgattt ttttggaact ttaaaaagta tagttatgga tttaaatata
     4081 ggaaagttta atattttatg gggtggacta gagaataaat gggagagcta ctatttaaaa
     4141 aatgacatta aatctataac acagaggata aacataaata ttgaaaaagc accatctgac
     4201 ttttttgatt tcattgaata aaaaatgtat tcaattattc taaataatta ggacatataa
     4261 atcaactaaa agtttttatt tatcgtattg aattagttat aatattttag tatagtaaaa
     4321 aatagtgcta ttttacatat ttaaagagtt taagtagaag agaggtgcca tattggatta
     4381 cttaaagtat tttgaaaaag ctgtaatgta tattgagaat aatttacatg aaaatataac
     4441 tgtaaatgat gtggcaaaag aaactggata ttcttattat catttaacac gcttatttaa
     4501 gtctatgttt ggtgaaagtg taggaagtta tataaaaaaa agacgattag taagtagtac
     4561 aaaagaactt ttatatagtg ataaaaaagt gatagatata gctattggta gtggatttga
     4621 atcttcagaa gcatttagca gagcatttaa atctatatac aaggttagcc ctatagaata
     4681 tagaaaaaat agaattgatg tatttgttgg taaaaagaaa aaattagagt tagattttat
     4741 gagacatctt gttggaaata taacgattaa accagttata aaagaaatag aaaaaataaa
     4801 ggtaataggt ataaaggata aagtaatttt agaaaataat tcattacctg acttgtggga
     4861 gaaattcaga aaagtacatc atattgtccc aaatactttg ccatcaaaga gagtttttga
     4921 aatctgttaa gcaatctctg aaatacacct agttagtgaa agtatggaat ttaatgagat
     4981 aataggactt gaagttaata gttatgatgt gattccaaat tcatttgttt caaaaactat
     5041 taaggaagga aagtatgcta tatttactca tactggtagc ttagataatt tagacaaaac
     5101 ttatgaatat atatggggta cttggttttt aagttcaaaa gaagaacttg atataagaga
     5161 tgattttgaa gtatatgatg aacgattttt aggtcctagt aatgtgaact cacaaattga
     5221 tatttatata cctataaaat aatctataag aaaactaaaa aataatttga ttaagaaata
     5281 gatgttcgta aaagagcatc tattttttat ttttatcaga tttagataat tttaataata
     5341 aaagaaataa aaatatatta taaaaacaat ttgattggaa ttttcagaaa attaatgcta
     5401 taattaaaag taaatttaaa gggaggttat aaaatggcta aattaactct agaacatata
     5461 aaaggaaata catattatat tccactccct acaatagtag gtgtatatgt agatggtaag
     5521 gatgcaattt taattgacag tggaaataat aaggatacag ctagacaagt tttaagatta
     5581 ttagaagaac ataatttaaa tcctaaatta ataataaaca ctcattcaaa tgcagaccat
     5641 ataggaggaa atgcatattt gaagaatcaa acaaaatgca agattgctac gactaaaatt
     5701 gaaggatatt ttacagaaaa tccaatatta gaatcagcat ttttatatgg tggctatcca
     5761 agtaaagcac tgaagaataa atttttatta gcaaaggaaa gtgaagttga ttatattatc
     5821 ccttcaaatg gcaaaattgt agatacagaa cttgaagtga tttctcttcc aggtcattat
     5881 tttgaaatga ttggtgttag aactccagat aatgttttgt ttgttgcaga tagtcttaca
     5941 ccagagaata taattactaa atatcatttt ttcttcctac tggatataga ttcacagttt
     6001 aagacactag aaaaattgag aatgttagag gctgatttct ttgttccaag tcactctgtc
     6061 aaaactacag atataaaaag tttaattaat attaataaga agaagatgga agaaataata
     6121 gataatatta aaaaggtttg ttatgagcca gttatgattg atgaagttat tgaaaaaatg
     6181 tgtgatttgt acaatgtcaa gcttgatgct aatcaatatg tgttggttgg aagtacaata
     6241 cgttcttata taacttattt atatgagaat aatatggttg aatatatatt tgatggtggt
     6301 aagatgatga taaaagctgt gtaaatagta ataattttca aattatatga tgaaattgtt
     6361 ttagtatgac tgttatttga aaagtaatat aaaaaaattt atataaataa gctaagatga
     6421 ttgatataaa gttactgata attttaaggt aatatatacg ttactataat attatgttat
     6481 tatttgtaaa taaggggata atttataagc aaattaaaga ccttaattta caggaggaat
     6541 agtgtatatt atgaataaaa aattcaaaaa attcagtaaa aaactaagta tattaatttt
     6601 agttttgact atgtttcttt ttaataattt aactgcaaat gcacagatta aagaagattc
     6661 aataatagga attgatagat atgaaacagc aggacttata gcggatagac aaacttatga
     6721 tactgtaata ttagttaatg gagataaaaa tttatctgat gggctttcat caagtggatt
     6781 agctggagct attaacgcac caatactttt gactaaaaaa agtgaaatac caaaagctac
     6841 tttaaataga ctagataata agagtttaaa tgttgtaaaa aaagtatata taattggtgg
     6901 ttataatact attgaaaatt cagtagaaaa agatataaaa agaaaaggta ttcaagtgga
     6961 aagaataaat ggtaacaata gaattgaaac tagttataat gtagttaaaa aaataaatga
     7021 agtaagtagt gtaaaagagg taatgcttac aaatggattt gtaggagagg ctgatgctat
     7081 gagtatagca cctgtagttg caaaaaataa aggagctata atacttacag atgggaaatc
     7141 agttccattt aagactgaaa gtttaaatgt ttacgctata ggtggcaaat ctgcgataaa
     7201 tgaggattta gtaaatgaaa ctaaagcaac tagaatcgga ggaaatgata gatttgagac
     7261 caataaaaag gtaatggaaa agttttataa tggagcgaca gatttttata taacaaatgg
     7321 atatcaatta gtagatgcac taacattatc tcctttagcc aaagaaaaac caatcgtttt
     7381 agttgctgat ggaagtaata aaggtatttt aaaaggagct aaaagtataa caaaagttgg
     7441 aggtattgat gccaatatat ataaacagtg tttagatatg attaaatata ataatatgga
     7501 tatgactcca aatattttaa aacacttaac ttcagttgaa ggaaatgaag tttctgaaat
     7561 acttatagaa tcagataatt taggtgtggt tgttgaaaag acgaatacca ataaatttga
     7621 gtttgattac gtaagtgtaa ctaatgaaaa aaattgtact ttttctgtat ataaagaaaa
     7681 ttcttctaat aatataaaaa atggaaagct agttgtaagt gctaagaaaa aaatagataa
     7741 gaaagatagt gatttgcaag atatgaatgg cgacaatatg ataaatgcaa ataaagatag
     7801 gatggtaaat gtgataaaaa ttggagttcc cgataaaatg tattctaatt ttaaagtaga
     7861 tgttgaaagt ggttcagtag aactttataa tattaaagga ggagctactg ttgatgtcaa
     7921 tgacgggatt gctaacattg ttgataacaa tgtaacaaat cctttcaata taaatactaa
     7981 tagtgggatt tcaggtgtaa cagctgaaac tatatcatct gaaataaaat ttagagcaaa
     8041 caatggaatt gtaaatgtaa cagctacgaa tataaatggc aatataagtt tatttggaag
     8101 cgataataaa ggtacctttg atggaatatt taaattaaat ctgaaaaaag aaccatcaaa
     8161 tttacacttg aagttaattg gaaatgggat aaataaattg ccagaaggtt ggtctaaaaa
     8221 ttatatttta ggaaatggac aaccaattat tgaagttaaa aataatggca taaataatat
     8281 atcaattgga gaataaaaac atatgaa
//
LOCUS       DAMJYC010000141         8291 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJYC010000141 DAMJYC010000000
VERSION     DAMJYC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.147"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            50..205
                     /locus_tag="PC472_003598"
     CDS             50..205
                     /locus_tag="PC472_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728892.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            335..1348
                     /gene="aroF"
                     /locus_tag="PC472_003599"
     CDS             335..1348
                     /gene="aroF"
                     /locus_tag="PC472_003599"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2728893.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1370..2434
                     /locus_tag="PC472_003600"
     CDS             1370..2434
                     /locus_tag="PC472_003600"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2728894.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2434..3747
                     /gene="aroA"
                     /locus_tag="PC472_003601"
     CDS             2434..3747
                     /gene="aroA"
                     /locus_tag="PC472_003601"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2728895.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3725..4804
                     /gene="aroC"
                     /locus_tag="PC472_003602"
     CDS             3725..4804
                     /gene="aroC"
                     /locus_tag="PC472_003602"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2728896.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGMELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4807..6003
                     /gene="pheA"
                     /locus_tag="PC472_003603"
     CDS             4807..6003
                     /gene="pheA"
                     /locus_tag="PC472_003603"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2728897.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRALDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTKEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6050..6859
                     /gene="aroE"
                     /locus_tag="PC472_003604"
     CDS             6050..6859
                     /gene="aroE"
                     /locus_tag="PC472_003604"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2728898.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6871..7404
                     /locus_tag="PC472_003605"
     CDS             6871..7404
                     /locus_tag="PC472_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2728899.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7425..8264
                     /locus_tag="PC472_003606"
     CDS             7425..8264
                     /locus_tag="PC472_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2728900.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3407 a          829 c         1500 g         2555 t
ORIGIN      
        1 aattattaaa caagaagaat aataaattat aaaaatggag gataatacta tgaaaggaat
       61 attaaaagaa ctaacagcaa aactttatag ctttttttac tttatgttct ggggctttta
      121 ttttagcgct ggctatttct tttacaaaaa tatatgtact tcctttctgg tgaggcctta
      181 ttttagtgat tttgagcata agtaaatttt ataaaaatga ttattttata tatttatata
      241 agatttagtg cttgatagat tattttagag aactcattag tgagttctct tttttatttt
      301 aaatataaaa ttataatatt gggaggtagt aaaaatgata gtagtattaa aaatgggagc
      361 agataaaaat gaagttaaaa aattaattga ggctattggg agagaaggag tagaagttaa
      421 tcccatagat ggaacagaat taactgtttt aggtttagtt ggagatacta gtaaaataga
      481 tgccaaaaga atagaagcaa ataaggtagt tgaaaaagtt atgcatgtgg tagagccttt
      541 taaaaaggcc aatagaaaat ttcatccaga accttcaatc ataaatgtta atggaatgga
      601 aattggttca aagaaaattg ctatgatagc tgggccttgt tctgttgaaa cagaagacca
      661 aatagtatct atagctaaag atgtgaaaaa atcaggtgca ggatttttga gaggtggagc
      721 attcaaacca agaacatcac catacgcttt ccaaggattg aaatatgatg gattggattt
      781 attaaaaaaa gcaaaagaaa aaacagggct tcctatagtt acagaaataa tgtcaacaca
      841 agatatagat atatttgaag aaaatgtaga tgtaattcaa gttggagcta gaaacatgca
      901 aaactttgat ttattaaaag aattaggtaa aacaaataaa acaatacttt taaaaagagg
      961 attatctgct acaattgaag agtggctaat gtcagcagag tatataatgg ctggaggaaa
     1021 tgaaaatgta gttctttgtg aaagaggtat aagaactttt gaaacatata ctagaaatac
     1081 tttggattta agtgcaattt tagctgtgaa gaaacttagt catttaccag ttatagttga
     1141 cccaagtcat gcagcaggta aatcatggat ggttgactct ttatctaagg cagcaattgc
     1201 agttggagca gatggtctaa taatagaagt tcataatgac ccagcacatg cactttgtga
     1261 tggaaaacaa tcgattaagc caaatgaata tgatgaattg ataagtgaat taaagactat
     1321 cgcatcagca gttggtagag aaatataatc tataaaagga tggttaaacg tggaagaaaa
     1381 actattagta aatttaaaag aaaatagcta caatatattt attaaaaaaa atatactaaa
     1441 gaatatagga gaagaaataa aaaaaatata ttttggaaat aagattttta ttgtaactga
     1501 tgaaaatata gcaaaattat acttggataa agtaaaaaat aatttaagtg aatctggatt
     1561 tgaaattaaa tctttaatat taaaatctgg tgaagaaaca aaatcattta atactcttcc
     1621 taaaatatac gatagtttac ttgactttaa gttaactaga aaagatttga ttattacttt
     1681 aggaggaggg gtaataggtg atttaggtgg atttgcagca gcaacttttt taagaggtat
     1741 aagttttatt caaattccaa catctttatt agcacaagtt gatagttctg taggtggaaa
     1801 agttggagtt gaccttgaga gaggcaaaaa cttagtggga agcttttacc aaccaaaggc
     1861 tgtgtttatc gacccaaatg tattattatc acttccagaa aaattctata gagatggaat
     1921 ggcagaagtt ataaaatatg gatgtataaa agacaaaact tttttcaata aacttttaag
     1981 tttaaaaaat agagaagaag taatgcaaca tatagaatac attatacata aatgttgctt
     2041 tattaaaaaa actgtagttg aagctgatga aaaagacttt ggagatagaa tgcttctaaa
     2101 ctttggccat acattaggtc atgctattga gaaatactac aattttacag gttataccca
     2161 tggagaagct gttgccatag gaatgtataa gataacttta gaaagtgaaa agataggaat
     2221 tacaaaaaaa ggtacatcag aacttataaa atcaatttta atgaattata atttgcctta
     2281 tgagattgaa ttaagagata ataaatctat agtagatgct atatctcttg ataaaaaaaa
     2341 tctaggtgat acattaaaaa taatactttt aaaagatatt ggagagagta ttatatataa
     2401 tactacttct aaattttttc aggaggtaag ataatgggaa gtctaaaaat atatccaagt
     2461 aaattatctg gagatgtaaa aataccacca tcaaaaagta tggcacatag ggcagttatt
     2521 tgttcatctc taagcaatgg aaaaagtaga atttctaata tagatttttc tgatgatata
     2581 atagctacta taagagcaat gacatcatta ggagctatta tagaaaagaa agaagatatt
     2641 ttagaaattt cgggaatttt ttctaaagaa ggtattttaa atagagagaa tcagttgaat
     2701 caacctaaat tgacaataga ttgtaatgaa tcaggctcta cattgagatt tttagtacct
     2761 atatctttaa catttgatgg tgtaaaaagg tttataggta gaggtaatct tggaaaaaga
     2821 ccacttgata cctattatga gatatttgac agacagaata taaaatatag ttacaaagaa
     2881 aatcagttag atttgattat atcaggtaag cttaaaccag atgaatttag agtaaaagga
     2941 aatataagtt ctcagtttat cacaggttta ctgtttatat tgcctacgct agaaagtgat
     3001 tcaaagataa taattactac tgagctagaa tctaaaggat atttagattt aactctttca
     3061 acgataaaag attttggggt agaaattata aacaataact ataaagaatt tattataaaa
     3121 ggaaatcaaa cgtataaagc gagagattat aaagtagaag gagattattc tcaaggagca
     3181 ttttatttat cagcagatgc tataggagaa gatattagca tattagacct taaagaagat
     3241 tctcttcaag gggatagtga agtagttgaa attttatcta ggatgggaat ggaaatatta
     3301 agagaaggca acaaaataaa aggaataaca aatggtttaa atggtacttt gatagatgca
     3361 tctcaatgcc cagatataat acctgtatta tctgtagtag catccttaag tatagggagg
     3421 actacaataa ttaatgctgg taggcttaga attaaagaat gtgatagact tcatgcaata
     3481 aatgttgagt taagtaaact tggagcaaat atagaggaga aagaggatag tttgataatc
     3541 gaaggagtaa gcaagttaaa tggtggagta gaggtgtgga gtcacaaaga ccatagaatt
     3601 gctatgacac ttgcaattgc ttcttgtaga tgtgataaac caataatatt aaaagatttt
     3661 gagtgtgtat caaaatctta tccacatttt tttaaagatt ttaagatgtt aggaggtcga
     3721 attgatgagt ggaatatggg gaaataattt aaaagtatca atttttggag aatctcatgg
     3781 aaatgctata ggtataaata tagatggtct tccttcgggg atggaacttg accttgataa
     3841 tattgataaa gaaatgaaga gaagagcacc tggaaaaaat tctatatcta cttcaagaaa
     3901 tgaaagtgat ataccagaaa ttctaagtgg atattttaat ggaagaacta caggaacgcc
     3961 attatgtgca atcataagaa atagtgatac tcgttcaaaa gattatggag agcttaaaaa
     4021 tttaatgaga ccaggacatg cagatttcac aggtaatgtg agatattctg gatttaatga
     4081 ctatagaggg ggaggtcatt tttcaggtag gataacagct ccattagtat tttgtggggc
     4141 tatatgtaaa caaattttat cacaaaaagg aatagagata ggagcacaca taaaaaaaat
     4201 taaaaatata gaagatatga gttttgatta tgtaaatatt tcaaaacaac agttatcaaa
     4261 tttgcaaact ttggaactac ctttacttga tttatcaaag gaagaagcca tgaaaaatac
     4321 gattatagat gcaaaaaatc aaggagattc agttggtggt ataattgagt gtgcagtagt
     4381 aggtattaat gtagggcttg gaaatccatt ttttgactct gttgaatcta ctttatcaca
     4441 tttattgttt tctgttcctg ctgtaaaagg tgtagagttt ggtttaggtt ttgaacttgc
     4501 agatatgtat gggtctcaaa gtaatgatga aatgtattat gaaggaaatc aagttaagag
     4561 caaaacaaat aacaatggag gaattatagg agggattact actggaatgc caataatttt
     4621 caaagtggca attaagccaa ctccatcaat tagcagacaa caaaacacag ttaatataaa
     4681 agataaaaag gacgatattt tatatattaa aggcagacat gacccttgta tagttcaaag
     4741 ggctatacct gtaatagaag cagtaacagc cattggaatt tttgatttga tgaaaggacg
     4801 ataataatgg aggatttaac gagatataga aatgagattg acaatataga taaagagctt
     4861 attcaattat ttgaaaagag aatgaataca gttcttgaaa ttgccagaca taaaatgaaa
     4921 aataacacta ctatacttca aaaagataga gaagaaaaag tactaagtag agccttagat
     4981 aatttagata ataaagcata ttcacaagaa acggtacagt tttttaattc tataatggaa
     5041 attagtagag gattacaaaa aagattgata gataatgatg gtgagcagaa aatcgagttt
     5101 agtttagatt catcaaataa gaaatttgat ttgtcaaatg taaatagata taagtcttta
     5161 aaagatgaat taaataaaaa aaatatccta gtagggtttc caggaaaatc aggttcattt
     5221 acaaaagaag cactaaataa attcttcaac aaaaaaatat caaaaaaaca atttaaagaa
     5281 tttgaagatg ttttcatagc acttaaaaat aaagaaataa actatggaat aatccccata
     5341 gaaaattcat ctacaggagc tatctctgaa acttatgacc ttttgagaaa gtacggtttt
     5401 tatatagttg gagaagaatg tataaaaata gaccaaaatt taatagggat aaaagacaca
     5461 aagcttgaag atataaaaga aatatattct catccacaag gtataggaca atgtagtgaa
     5521 tttttaaaac aaaatagtac atggaaattg ataccattta ataacacagc aacaagtgca
     5581 gaacttgtaa gaaaacttca agataaaaca aaagcagcaa ttgcaagcaa aaaagctgca
     5641 agtatttatg gattagaaat aataagtcct tgtataaatg atattgcaaa caactataca
     5701 agatttgtag taatcagtaa tcaaatacac attgaagaag aatcaaacaa aatgagtgta
     5761 gtattttctg ttgaacatga agcaggaaaa ctttataaag tacttggata ctttgctaaa
     5821 aataatataa atatgacaaa aatagaatca agacctatga aaaatgcctc atggagatat
     5881 ttcttttata tagattttga gtgttctata tacaatgcta aagtgtacga tttattagaa
     5941 ttgatagaac ataatacagc atattttaaa tttatgggag tatataaaaa taaaactgaa
     6001 tagataagat aaatttttat ttaatttata attattagga ggtgtaagta tgaatttttt
     6061 cggtctagta ggtgaaaaac tctctcacag tgtttcacct caaatccaca agagagtatt
     6121 cgagatattg aatattgaaa gtgcttataa aaactttgaa atttcaaaag aagatatatc
     6181 taagctagac ggtgctataa aattattagg aatacaaggg gtaaatgtta ctgtaccata
     6241 taaggagcgt attatgaaat atcttgattt tatatcacca gaggcaaaac gtataggagc
     6301 agttaataca attttactta gagaaaatat gttatatggt tacaatacag attactttgg
     6361 tcttgatagt atgtttaaaa tggctaatat tgatgttcaa ggaaaagtag cagttatatt
     6421 aggaactgga ggtgcttcta aagctgctct tacatatttt attgattctg gtatagaaaa
     6481 gctttatgtt tcaactagaa aaaaggatga taaaaaatta ttaaatagta aagctatatt
     6541 aatagattat gaagaactta aacatataaa aggagatata atattaaatg ctacaccagt
     6601 aggtatgtac cctaatgtag gaatttcacc agtgtcaaaa tctattatac aaaattttga
     6661 tatattaata gatttaattt acaatcctgg agaaacagag tttttaagaa ttggtaatag
     6721 tatgggtaaa aaaacttgtg atggattata tatgctggtg gggcaagcaa taaaatctca
     6781 ggaaatttgg caagatacta aaatagataa tagtatttta gatgttatat ataatgaact
     6841 aaaattagaa tttttgtaaa ggagattgtt atgataaata aaacaaaaga aaaattgata
     6901 ttaataggta tgccaggtag tggaaaaact acaattggta aacttttggc caaagaatat
     6961 aattgtagtt tctgtgatat ggatgattat ataattcaaa tatctcaaaa aagtatagca
     7021 gagttgtttt cagaaggaga agatatcttt aggaactatg agactcaagc atgtagagaa
     7081 ctttctataa gtgataaaac agtgatatct acaggtggag gtgtaataaa aaaggatgtt
     7141 aatatggaaa tactaaaaga aacaggtata ataattttta ttgatagacc tatacaaaag
     7201 attttggaag atataaatat taattcaaga ccattattaa aaaacggaaa agatagattg
     7261 tataatcttt acaatgagag aataaatctt tataaaaaat tttcagatat agagatttta
     7321 aatgataaat cattaaataa tgctgtatat aatattacaa atgcagtcag tgaaaatttt
     7381 aaatttgact ttaaagaaaa ataatctagg gggaagtaaa aataatgaat attgttattg
     7441 taggactagg agttatagga ggctcttttg ctaaagcatt aaaaaaagct ggatatgaaa
     7501 atgtatttgg agtagatgta gatttagaaa ctttaaaaaa agcagaaaaa gcaaaaatca
     7561 taaaaaaagg atgtactaca ggtaaagagt tatttaaaaa agctgattta attatactat
     7621 ctatatatcc tagattagtt gtagattttc taaataataa taaaaacttt tttaaaaaag
     7681 ggactataat tactgatact acaggaataa aagaaacact tatcaatgat gtgttgcaaa
     7741 ttattccaga tgatatagat tttatatttg gacatccaat ggcaggtagg gaaaaaaagg
     7801 gtattgattt tgcaagtgaa caagttttta atggtgcaaa ttatataatt actccaactg
     7861 gcagaaacaa cataaaaaat ttagagttag ttgaaaattt aattttagag attggattta
     7921 aaagagtaaa aaagttaact tcacaaaaac atgatgagat tatagctttt acttctcagt
     7981 tacctcatgt tatggcagtt gctcttataa atagtgatga agaaggtaga gatacgggta
     8041 aatttattgg agatagttat agagatttaa ctagaattgc aaatatgaat gaagacctat
     8101 ggagtgaact ttttttagga aatagagata atttactaaa agttatagaa aactttgaat
     8161 cagaagtaaa tttagtaaaa gaagctatat ttaacaatga taaaaataaa ttaatagagt
     8221 attttaaaaa atcatcaata agaagagaag ctttagaaaa ataaatatta atatttaagc
     8281 tatatataga t
//
LOCUS       DAMJYC010000142         8177 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJYC010000142 DAMJYC010000000
VERSION     DAMJYC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.148"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..597
                     /locus_tag="PC472_003607"
     CDS             <1..597
                     /locus_tag="PC472_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2728901.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC472_003608"
     CDS             728..1750
                     /locus_tag="PC472_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2728902.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC472_003609"
     CDS             complement(1914..2471)
                     /locus_tag="PC472_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2728903.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC472_003610"
     CDS             complement(2477..3553)
                     /locus_tag="PC472_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2728904.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC472_003611"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC472_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2728905.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC472_003612"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC472_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2728906.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDNGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC472_003613"
     CDS             5391..6320
                     /locus_tag="PC472_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728907.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC472_003614"
     CDS             complement(6481..6930)
                     /locus_tag="PC472_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728908.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC472_003615"
     CDS             complement(7133..8113)
                     /locus_tag="PC472_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2728909.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2830 a         1348 c          983 g         3016 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggggta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gtccaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggtgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgattgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttacaggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattactt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacctttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat tatctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atgtggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttcct cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcact acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat tacacta
//
LOCUS       DAMJYC010000143         8131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJYC010000143 DAMJYC010000000
VERSION     DAMJYC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.149"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(109..960)
                     /locus_tag="PC472_003616"
     CDS             complement(109..960)
                     /locus_tag="PC472_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2728910.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1009..2178)
                     /locus_tag="PC472_003617"
     CDS             complement(1009..2178)
                     /locus_tag="PC472_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2728911.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2649..3566
                     /locus_tag="PC472_003618"
     CDS             2649..3566
                     /locus_tag="PC472_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2728912.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC472_003619"
     CDS             complement(3695..4624)
                     /gene="pfkB"
                     /locus_tag="PC472_003619"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2728913.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            4891..5616
                     /locus_tag="PC472_003620"
     CDS             4891..5616
                     /locus_tag="PC472_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2728914.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5699..6853
                     /gene="nagA"
                     /locus_tag="PC472_003621"
     CDS             5699..6853
                     /gene="nagA"
                     /locus_tag="PC472_003621"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2728915.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7552..8034)
                     /locus_tag="PC472_003622"
     CDS             complement(7552..8034)
                     /locus_tag="PC472_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2728916.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT         2863 a         1179 c         1025 g         3064 t
ORIGIN      
        1 tttcatcaat taaatgaaaa aaatacacca tttacatata attaatataa atttttcata
       61 taacaaatat cctaaactta tctaaaataa atttaggata ttttattttt atactctatt
      121 tacagaccca cacactttaa tcttttcttt cacaaaatcc tttactgatt gaagccctgc
      181 taatccatac tttttagggt ctataacctc agaatcttca tctaaaactt ttttaacccc
      241 tttactatat gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat
      301 agcttctttt ataatatcat catgtattcc tgaacctcca tgcaatacta aaggtattga
      361 aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa
      421 tccatgtgca gtacctatag caatagccaa tgaagatatt cctgttctat cgacaaactc
      481 ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt
      541 tccaccaact ttcccaagtt ctgcttccac tggtataaga gaaggactac atgcttttac
      601 tactgattta gatatagata tattattttc aaagctttca tgagaaccat ctatcattat
      661 tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag
      721 atgcattgct acaggtatat ttactctttc tgcagcaacc tttgcatttg ctagataata
      781 atcaagacct gcatatttta cagtagatgg agtagtttgt aatattactg gagaatttaa
      841 ctcctcagca gcttctataa cagcaattac catttccata ttttctacat taaatgctcc
      901 tactgcatac ccttcatctt gtgccttttt aagcattgat gttgttgtta ctaatgccac
      961 attgataccc tcccaaatta tttagttata atattttaat cttatctttt aatattcata
     1021 tattgttaca ccttttacta ctcgatttac tgttcctgta gctgatggag tatctggctt
     1081 atttccaacc tttaccgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat
     1141 tgcaagatac acatctggta atttattata cttttcttta aactcaaact ttgttcctga
     1201 gaagttattt tcattttcta ctgacactgc acaagtgaat ttagcaattt tatccctttt
     1261 tatctcctct aaaacatcta aatcatattt tcttgtatat aaacagttag atacaaattc
     1321 aaatacaagt gtattttcat caataaatga ttttggtccg tgtctaaacc ccataggaga
     1381 atcatacact gtagatattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg
     1441 tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcaaaat ctttatttat
     1501 taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt
     1561 tatatatgac ttttctacat catcttctaa actatcgaat attaacatag cagataacat
     1621 catacacgaa aaacttcctg tcatggcaaa gccttcatca tttgattttg atggcattaa
     1681 taacaagtaa ttattttcgt cgtttttagt catcttagcc agttctcctt ctggattaca
     1741 agttatagct aaatgataaa aattatctac aatcttattc cctaaattta aagcagccaa
     1801 actttcagga ctatttccac ttcttgcaaa tgaaattaat aatgttggta tatctttttt
     1861 taagtaatca tatgggtttg aaactatatc cgtagtaggt attgcttcaa atatgtattt
     1921 tcttatgtca tttttatttt taaggtaagg aagtattgaa tttcctatgt aagctgaagt
     1981 tccagcacct gtaaatataa ctctaaactg cccatgatta ttgcttattg tatctatgaa
     2041 tctacttaat ttttctttgt tagatttata tatttcataa gtttctaacc ataattcagg
     2101 ctgttgtttt atttcattag ttgttattat tgcacctaaa tcttttagtt tttgttcttc
     2161 taatttaaac attttcattc tcctttcata cttgtaacgt ttacatttgg taatgaggtt
     2221 ataaccactt taaactataa aaaaatatac tcttttataa ttttttgctt agatttttta
     2281 gtactactta aactcactat aaaatataca ctatagattt taattaattt tatagtttat
     2341 ctttaaaaat ttaataaaat catactctat atactttagt atttttattt tacgataaaa
     2401 acttgttcag accaaagtgg taattaccac taatccaaat ataaattaat ttgaattttt
     2461 tgtcaataca taaaatattt atatcaatca attttatgga tatcctacaa aattacttta
     2521 tcaatatttc ttatttcgct aatttactta tataagcttt aaatttattt tttgatacaa
     2581 agtttacaaa tttatgtgtc tgaattataa tcaattctgt aaatataaaa accttaggag
     2641 aaagtattat gaaaaatata aaaatgggaa tgataggtct tggctccata gctcaaaagg
     2701 catatttacc tatattaact aagtctgaga gatttgagtt tgtaggagct tttactccaa
     2761 ataaagttaa aagggaaaaa gtctgtagtg attatagaat catgccattc gattctattg
     2821 aatccctagc taaaaaatgt gattgtattt ttctacacag ctctacagaa acacactacg
     2881 aaattataaa aatattatta aatttaggtg ttcatgtata tgtagacaaa cctcttgctt
     2941 caacagtaag tcaaggagaa gaacttatcg aattaagtac gaaaaaaaac ttaaatttaa
     3001 tggttggatt caaccgtaga ttctgtccta tgtacaaaga aattaaaaat aatgctacag
     3061 aaattgtatc tatcaatata tgtaaacatg gattaaattc cttaagaaat gttaaattcg
     3121 acagtactct tatagatgac tatatacatg ttattgacac tgctttatgg ttagcaaatg
     3181 aagatgttga aattagtgga gaggatttat ttttaacaga caataagaat cttatatttg
     3241 taagtcataa acttaaaggt aaaaatttct ctataaacac ctctatgcat agagattctg
     3301 gaacaaagtt agaacaagtt gaaattcttt caaagggtaa tattcaaaga gttaagaatc
     3361 ttaatgtact ggaaatagaa gaaggtggaa acctaacttt aaagcaaagt ggtgcatggg
     3421 taaacatatt aaaacaaaag ggttttgagg atatatcaaa tcattttatt gattgtatag
     3481 aaaataatat aaaaccagct attaatggag aagagtgtat caaggctcaa agattactag
     3541 agaaaattat aaattcagta aaatagatat ttattataaa aataggttaa aaatgaattt
     3601 ttctaaccta tttttatata agactatatc ttttatatta aaattaaaat ctattacaac
     3661 aacattaaat ttattatatt cccattaaat gtatttaact taactttttt acttcaacta
     3721 aatcttgata tttatcaatt tctaccaaat ctacaaatcc agtttccata tttaaagcat
     3781 tacttatacc acaagcattt gcaaatttaa gtaaattttc tattttataa ccccttaaaa
     3841 tacctacact aaatccagcc actgtgctat ctccactacc tacagtattt atacacttta
     3901 caactggtac ttttacatca tatacattat cttcgtttaa atacaccata ccatcttttc
     3961 ctaaagatat acatacattc tctgcaccca tttcaataag tttttttcct gacaagataa
     4021 tttcttctct tgattctata ttcattccta ataaatgtct tatttcatct atatttggct
     4081 ttattaaata tggttttgat tttaaagcta tttttaatgc ttctcctgat gtatctaata
     4141 taaactttat attatttgct ctacatatat ttcctatctt ttcataatag tctatcggca
     4201 tattctcaca ataactacca gaagctacca gtattttagt attttttagt acttcctcta
     4261 tatcattttc aaatctctct aaatcgcttt tttctactat tggtcctttt tctaaaaatt
     4321 ctgtactaac tttattgtcc tcaattatgt ttaagcaatt tctagtttcc tgctctattt
     4381 ttgtaaattt attttgtata tctagttttc taagttcatt ttttataaat tctccattaa
     4441 atccgcctag aaatcccata gctaaaggat tttcttttaa aattttacat accttagcta
     4501 cgtttaatcc tttacctcca gcagttgcat tcttacttac taccctttgc acctcaccta
     4561 tgtttatatt attcactcta tacattctat ctatagatgg gttgaatgtt attactgtaa
     4621 tcatactaga ttctcctcat tattaatttt tacttttcta aacaataaat taatctgtaa
     4681 aataaaaaat acctcatata atatatccaa agtggttatt accaatttga atataaatta
     4741 ttttacttgt tttgtcaatg taccataatt ttataataaa ttaatagcct tacttattga
     4801 attttgatta aactttattc attgacataa ataattctat attctataat caaattgtgg
     4861 taataaccac ttagaaagga ctatattttt atgataaaaa gaaatgataa aagaccaata
     4921 tatgaccagc ttgttgagat attaagaact aagattgaaa atgagatgga accaaacgat
     4981 agaatgcttt ccgaaagaaa aatttgtgat gaatatggag taagtagaac cacagttaga
     5041 ctagctatgg ctgagttaga gcatatggga tatatataca aaagacatgg aaaaggaact
     5101 tttgttgctg cactaagtca aaattctcaa aacttaatgg aaagttatag ctttacagat
     5161 tatatgagaa cacaaggaaa aaagccaggt acaaaggtat tatcatttga ggtagtagaa
     5221 agtacaaagt atttctctga aaaattaggt ataactcctg gtgaaaaaat gataaaaata
     5281 atgcgtttaa gacttgctga tgaccttcct atgatgcttg aaagaacata tcttcctatg
     5341 aaagaattta taggattgac aaaagaaaaa atcgaacaaa aacctttata tgaaatattt
     5401 agagaagatt atggcgagat tattaaagtt gctgatgaag aattttcagc tggaataatc
     5461 tctgataagg aagctaagtt attggaagta ccaggagatt caccttgtct gaaattactt
     5521 cgtaccacat ataatgatga caatatagtt attgaattta ctttgagtgt cgctagaagc
     5581 gataaatttg tatatagagt aagacatgta agatagtaag ttaaatagct ttcaagaaaa
     5641 tagaatgata ttataagcgt caatgccaca atgatagtat aattataagg aggttatttt
     5701 gaaaagtttt atttatgcag ataaattttt tatgaaacat ggaatcaaag aaaaaggtta
     5761 ccttagtata attaatggca aattcggtga atttcaacaa gaaagaccta ataatactga
     5821 taatgttata gatttttcag gtaaatatat tgctcctggc ttagttgata ctcacattca
     5881 cggtctttta ggtgcagatg tcatggacaa tacatttgaa gcaataaaca caatttcaaa
     5941 aggcttatta aaatatggag taacatcttt cctacctaca actttaactg attctgtaga
     6001 tacactaagt gattctgtag aaaatataag caatagcttc aatcgagttg agggtgcaaa
     6061 aatacaaggg atttatttag aaggtccatt ttttacagaa aaatataagg gcgctcaaaa
     6121 tgaaaaatat tttaaagacc ctgacatcaa tatattaaaa ttatggcaaa aaaaatcgaa
     6181 aaatttaata aggaaaatag caattgctcc agaacgtaaa ggttcaatag aattaacaag
     6241 atatgcaact aaaaataata tatcagtttc tcttggtcat agttcagcta catttgagca
     6301 agttaaacaa gtcgttaatt caggtgctaa agtgtttgta catacatata atgggatgag
     6361 tcctttaaat catagagaac ctggaatggt tggggctgct atggtactaa aagatactta
     6421 tgctgaatta atttgtgatg gtcatcatgt aagcccaata gctgttaaaa taatgatgga
     6481 tgctaagtct agtaataaca tagctttaat tacagattgt atgagagctg gtgctatgga
     6541 ggatggaaaa tatactcttg gtaagtttaa tgtaaatgta aaaaatagtg ttgcaagatt
     6601 aaatagtggg agtttagctg gaagtgtatt aactatggac aaggctgtga gaaatatagt
     6661 tgattggaac atatcaactt tagaagatgc aataaaaatg gctacatata tccctgcttt
     6721 aagttgtaat attgataatg tctgtggctc tatatatcaa ggtatggatg cagactttat
     6781 agtaacagat aatgacttta atatacacga aacttatata aatggtatct gtaagtacaa
     6841 agttaataaa taggttcaat ataaggttat ggaggctgtc taaaaagagt ctggaaatat
     6901 ttaaagttat ttctccaaaa tctgatttaa cacttttatt tccatatcgt taataaatta
     6961 ttgcgataga ggttatttta aacaaatata tattataagt atttttgtga taatattttc
     7021 gataatatgt acatttaaag taacttttct attattattt gtaagttttt atcttgagca
     7081 aacatataaa tttttaataa agcatgagat aaaccctata tttcaattac taatgatgag
     7141 attttgattg ataacagata tattttcaaa tgtaaaaata ctaaaaatca tatcaatttt
     7201 aaagatattg taggtgtaaa taaacaagat aatactatag aaatttttac aaatgaaagc
     7261 acttattgta tatatctaaa agttctaagc attgagagcg aagaatatct aatagataga
     7321 ctaaaatcat tttgttgatt ttatatattt tatgttaaat ataaagcaga gtagttaaaa
     7381 ctaccctgct tttgtaatta aaatttaaaa ataataactc ataatacatt gaatttgagc
     7441 ttaacagtaa ctattgtaat aatagcatca atcttttgtg ttgaatataa tttatttaca
     7501 ctatagttaa gcatgatttc taatgctatt ttctctaatt tacaattatg attaaaattc
     7561 attaatatat atatatttat aagttgtatt gtttatattg tttattactt ttttaaatcc
     7621 actagctgta ataataaatg tcttttctgg tatccctggt gaaaagatta ttttatcatg
     7681 gttaaatata tcttgatcta ttacaagaga gatatttttc tcatatccaa aaggagatac
     7741 acatccaggc aaacattgtg ttttttgtgt taattcttct tttgaacata tagatacttt
     7801 tccacctaaa acatttttta tagtttttgg atctaatttt tttccttctg tggttacaaa
     7861 tatatagtaa tttccagacc tatcttttat aaataaactt ttactttcta ctccagtata
     7921 ttgtaatttt tctcttattt ctttagcttt ttcaaatgat aaaataggtt catggtcata
     7981 ttcttcaaac gttatatgat tttcttttaa aatatttttg atattgttgt acatatattt
     8041 tcctttctat atttaaatta atttatatat aatattcaat tataaagtga ttttataatt
     8101 tcttcaaaat atcataaaaa acattcacat g
//
LOCUS       DAMJYC010000144         8012 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJYC010000144 DAMJYC010000000
VERSION     DAMJYC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.150"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(208..1785)
                     /locus_tag="PC472_003623"
     CDS             complement(208..1785)
                     /locus_tag="PC472_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2728917.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIISLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKEDTVNEAFRMVTNKPITVNIKGDCSKTITVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGNIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="PC472_003624"
     CDS             complement(2082..4493)
                     /locus_tag="PC472_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2728918.1"
                     /translation="MRKYKSKKLSKLLALLTVYFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGEALT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGNDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            4721..5119
                     /locus_tag="PC472_003625"
     CDS             4721..5119
                     /locus_tag="PC472_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2728919.1"
                     /translation="MIFKKHKETILYLFFGTLTTFVNIISYLFFTRVVSLNFIIANIL
                     AWILAVLFAYFTNKFFVFESKVIEIAFLFKEFLSFVSFRLFSGITEMLIMYIMIDLLF
                     INDIIVKVFTNIIVIVLNYVFSKMIIFKKL"
     gene            complement(5180..5887)
                     /locus_tag="PC472_003626"
     CDS             complement(5180..5887)
                     /locus_tag="PC472_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2728920.1"
                     /translation="MIEATLFFSPHQDDEILSMGSAIIEHVEKSDTHVILCTDGSKSV
                     IRKVLDDGGACSYHIKDIHKYSLSEDEFSYNRDDEFKNSCKEMGVKESNIHIEENRAH
                     DGELNKEKAKEIILKYLEEYPNAKVKTVTPYKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYNDFKKVNPNVKVWKLANSQENKLLNAMNAYKKWNPESGKYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            complement(5958..6395)
                     /locus_tag="PC472_003627"
     CDS             complement(5958..6395)
                     /locus_tag="PC472_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2728921.1"
                     /translation="MEYVNLIFLSMIPIVELRGAIPMGLALNLNPVLVYISCIIGSTV
                     IGIPILLTFRYMLDYLKVKGYFKCFVDFVENKLHNRAKKLKSLSIVGIVLFVGVPLPT
                     TGTWSAACIASLLRMRLKDAILGIFLGNIVSGCVVFAISYHIF"
     gene            complement(6468..7862)
                     /locus_tag="PC472_003628"
     CDS             complement(6468..7862)
                     /locus_tag="PC472_003628"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDF2728922.1"
                     /translation="MIKENQKLLNNIQMIIDLIIILSSFILAYYTKFNLLAGVNSIGV
                     YGYIITAICSVVMYFILYNILDLYSAKRTSNLYKEIKEIINANIIGALILTLILFIIK
                     LINYSRIVLMLFVMYNVILTSLSRVVLRSILRKYRSKGFNQKHCLVVGATETGKKLIG
                     KIKKNKQWGYNIIGCLDDDLYHLDRFEEILILGTIDEIETILEKTHVDIVFITIDSEK
                     FYKFGEIIKQCEKAGVKTNIVPYYYNYVPSKPYMDDLDGFPIIDTRHVPLDNYFNNAC
                     KRLFDIIFSIIGIIITLPIMLFSILMIKLTSSGKIIYKQTRVGLNRKNFDMYKFRSMK
                     TQKEEEEITKWTTKNDSRKTKWGSIMRKTSIDELPQFFNVIKGDMSIVGPRPERPYFV
                     DKFKYEIPRYMIKHQVRPGITGWAQVNGYRGDTSIEKRIEYDLYYIENWTFLFDVKIV
                     FFTIFKGILNKNAY"
BASE COUNT         2487 a         1353 c          873 g         3299 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatattgaa ctacatattt
      121 atctataagt ctgtttgtat ataaacctgt ttaactatat aactttagaa caattacaac
      181 acacacttca cttaaatata catattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ctgacttata gaatctatag ctttttcaat
      301 tgaatcaacc ttagcctcta aatcctcaac ctgattaact tttttatcta tattaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatgatt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaat agtctttgaa caatctccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtatcct cttttggttt
      721 aaagtttata atgtcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttataatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tatctctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagctgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aagtttgtat cagtgtaaaa cttttctata actttagcat tagtctcatt
     1081 tctatctttt ccactcactc tttctgcatt aggaaggctc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatctctta taaatttatc
     1201 aaatgcttct actccatcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgctcca acactaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacctttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctaacactac
     1621 attagaacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaaacttat
     1921 ttttcattat ttaaatgata aaaatataaa ataacaaaaa agagtgcctc atgaactaaa
     1981 aacttcattt tgagacaccc ctttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat cttttcattc actattttcc taaaagagta
     2101 tttagttcat taaaagcact ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac tagaactact
     2221 ggcgaatcag ttttagcacc taatgctcct actgctaatc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgaactagg gtagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 atttttgatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgag acagatttta tactatttaa cttttgagct
     2641 agagatatac tagtcttgta tctatcatta cctgaaattc tttctaccga tatacccata
     2701 ttgctaagtt gagtttgtac attcttactt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactccgcc ttagtttttg aatctaagtt atcttttcct
     2821 gttaaaagaa ttggagagtc gattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcgtctc catttactaa tactgcattt tgtgaagaag tccatccttt ttggctaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagctt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaacttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatattagtt tcatatgcta aggttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taaactgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagagt ctgtttcaaa cataacagaa tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttac taagaccagc atattcaagt
     3481 tggtacattt ttttatcact atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tctgttagta gagtcttatc ttcataagaa atccagaaga atcctttcat agaattaaac
     3601 tctccccaac tgctttttac taaccatgct ccatttgatt ctggttttac atcagatgca
     3661 aagttatctt ttgaataatt atcatcccat cctactatcg ctacagcgtg atttaatgga
     3721 gctctcttat ttgtacagtt atatgctgtt tcatctttat taaaatatgt tgaataatgt
     3781 gcataacctg atgtcactga accatattgc attatagcat tttttacagt ttccttatct
     3841 ttattaagac gaacaacatc tgttacatta aattgagtag gtgcagtatc catatttgaa
     3901 ggtttagttg caccttgtgc ctcagattta agattatatg gtatatcttt ttctaactta
     3961 ggacctgccc atgctgtcag atatcctatg gctgttacat ttgaactacc tgacatatca
     4021 tctaagttcc atccatattt tccaccagtt gcccaccatc ttaaatgttc ttctgataag
     4081 tcatacgttc catatccttt taatttaaga tatgcttcta aagttgacat acctgaaaaa
     4141 gaccagcatg tattaagact tccttgattt tttgctggtg tagtaagacc aagattcatt
     4201 gggttgtatg ctacacttga tttatatgct gatggaagac tacttctacc cttatttcga
     4261 agtgttgtac cttccatttc atgtggaaaa ggtattactc cgttatattt tgctgtgtct
     4321 ccatttttga catcttctag gtattgaaga tagctttcag aatactctgc agtttctact
     4381 ccatctagag ttttatggtt ttctgctgag acaggtattg ttgacactat taaaaagtaa
     4441 actgtcaaca atgctagtag ctttgacaat ttttttgatt tgtactttct catgtttacc
     4501 cccttatatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 tgataaaata aaaattgtaa gtaaatatta attgtaaaat attataattt tattttttat
     4621 gatattttac aatatgtcta tttgttaact gatatgctat actaaagtgt attagtgttt
     4681 ttaaagatag ttatatttga ttagtttagg agggttatat ttgattttta aaaaacataa
     4741 agagaccata ttatatttat tttttggaac acttactact tttgtaaata taatttcata
     4801 tttatttttt actagagttg tatcacttaa ttttataatt gcgaatattt tagcatggat
     4861 tcttgctgtc ttatttgcct attttactaa taagtttttt gtatttgaaa gtaaggtaat
     4921 tgagatagct ttcttattta aagaattctt atcttttgtt agttttagat tattttctgg
     4981 tattactgaa atgttaatta tgtatataat gattgattta cttttcataa atgatattat
     5041 agtaaaagtc tttactaaca taattgttat agttttgaat tatgtattta gtaaaatgat
     5101 aatttttaag aaactataat aaaaaatagt ctgctgaagt agcagactat tttttattat
     5161 atatgaggcg aattgtaagt taaggagcat gtacatattg aatcttattt gactttaact
     5221 catcaaaatg tgattttaca gaatgatatc ctattgcata ttttccactt tctggattcc
     5281 attttttata agcattcata gcatttagta gtttattttc ttgactattt gctaacttcc
     5341 agacctttac atttggatta acttttttaa agtcattata atcatatggc tctacataaa
     5401 atcttaaatc ctttattttt ccttctttat ataactctaa tgctgcctca cctagtgctc
     5461 tatggtcttc atgtattcca cttgctttat atggagtgac tgtttttact tttgcatttg
     5521 gatattcctc aagatatttt aatattatct cttttgcttt ttctttgtta agttctccat
     5581 catgagctct attttcttct atatgtatat tactttcttt gacacccatt tctttacagc
     5641 tgtttttaaa ctcatcatcc ctattatacg aaaattcatc ttctgataaa gaatacttat
     5701 gtatatcttt tatatgatat gaacatgccc ctccatcatc caaaactttc cttatgactg
     5761 atttacttcc atccgtacat aatataacat gcgtatcact tttttcaaca tgttcgatta
     5821 ttgcacttcc catacttaaa atttcatcat cttgatgtgg tgaaaaaaat aaagttgcct
     5881 ctatcaatta cgttcctcct attctataaa taaaaataaa tgtttattta aaagctttac
     5941 tattctacta aatgtactta aaatatatga taagatatag caaaaactac acatcctgaa
     6001 actatatttc caagaaatat acctaaaatt gcatctttca atctcattct aagtaaagaa
     6061 gctatgcatg cagcactcca tgttccagtg gttggtaatg ggactccaac aaataaaaca
     6121 atacctacaa tacttagcga cttcaatttt tttgctctat tatgtagttt attttctaca
     6181 aaatctacaa aacatttaaa gtaccctttt acctttaaat aatccaacat atatctaaat
     6241 gttaataata ttggtatacc tattactgtt gaaccaatta tacaggagat ataaactaat
     6301 acaggattta agtttagtgc aagacccata ggaattgccc ctctaagctc tacaattggt
     6361 atcatggata aaaatattaa atttacatat tccaatatat ttctccttat tataagtaat
     6421 ttaattcttt aattgagatt tgttttagtt ataaaataaa atctatctca atatgcattt
     6481 ttatttaata tccctttaaa tatagtaaaa aaaactattt ttacatcaaa taaaaatgtc
     6541 cagttctcta tgtaatataa gtcatattct attctttttt ctattgatgt atctcctcta
     6601 tatccattta cttgagccca acctgttatt ccgggtctta cttgatgttt tatcatatat
     6661 ctaggtattt catacttaaa cttgtctaca aaatatggcc tttctggcct aggacctact
     6721 attgacatat ctcctttaat gacattaaaa aattgtggta actcatctat acttgttttt
     6781 ctcattatcg aaccccattt ggtctttcta gaatcatttt ttgttgtcca cttagtaatc
     6841 tcttcttctt ctttctgtgt tttcatagat ctaaatttat acatatcaaa attttttcta
     6901 tttaacccaa ctcttgtttg cttatatatt attttccctg aagaagtcaa ttttatcatt
     6961 aatattgaaa acaacataat tggcaatgtt ataataatgc caattattga aaaaattata
     7021 tcaaatagtc ttttacatgc attattaaaa taattatcta atggtacatg tctagtatct
     7081 attattggaa atccatctaa atcatccata taaggttttg atggtacata attataataa
     7141 tatggaacta tatttgtttt tacacctgct ttttcacatt gttttattat ttctccaaat
     7201 ttataaaatt tttctgaatc aatagttata aatactatat ccacatgtgt tttttccaat
     7261 atagtttcta tttcatctat agttcctaaa attaatattt cttcaaatct atctaaatga
     7321 taaagatcat catctaaaca tcctataata ttatatcccc actgtttatt tttctttatt
     7381 tttccaatta attttttacc agtctctgta gctcctacta ctaaacaatg tttttgattg
     7441 aaccctttac ttctatactt tctcaatata ctccttaaca ctaccctact taaactagtt
     7501 aatattacat tatacattac aaataacata agaacaattc ttgaataatt tattaatttt
     7561 attatgaata gaattaatgt caatattaat gcacctataa tattagcatt aattatttct
     7621 tttatttctt tatataaatt acttgttcgt tttgcactat ataagtctaa aatattatat
     7681 aatataaaat acattacaac agaacaaata gctgttataa tatatccata cacacctatt
     7741 gaatttactc ctgctagtag attaaacttt gtataataag caagtataaa agatgataat
     7801 ataataatta aatcgattat catttgtata ttgtttaata acttttgatt ttcctttatc
     7861 ataaaaacac cttttttcta tcaaatatat tattgtataa ttttagcata ctttgatatt
     7921 tttttgtatt taaattaaga ttgacataaa aatacaaatt atttctagta aaaaattcaa
     7981 atatcactaa tacaaaaaat gtatatttat aa
//
LOCUS       DAMJYC010000145         7995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJYC010000145 DAMJYC010000000
VERSION     DAMJYC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.151"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            27..101
                     /locus_tag="PC472_003629"
     tRNA            27..101
                     /locus_tag="PC472_003629"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC472_003630"
     tRNA            116..200
                     /locus_tag="PC472_003630"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC472_003631"
     tRNA            210..293
                     /locus_tag="PC472_003631"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC472_003632"
     tRNA            317..391
                     /locus_tag="PC472_003632"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC472_003633"
     tRNA            416..492
                     /locus_tag="PC472_003633"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            500..575
                     /locus_tag="PC472_003634"
     tRNA            500..575
                     /locus_tag="PC472_003634"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Gln,seq:ttg)
     gene            584..659
                     /locus_tag="PC472_003635"
     tRNA            584..659
                     /locus_tag="PC472_003635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Lys,seq:ttt)
     gene            662..750
                     /locus_tag="PC472_003636"
     tRNA            662..750
                     /locus_tag="PC472_003636"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Ser,seq:tga)
     gene            754..829
                     /locus_tag="PC472_003637"
     tRNA            754..829
                     /locus_tag="PC472_003637"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Phe,seq:gaa)
     gene            836..909
                     /locus_tag="PC472_003638"
     tRNA            836..909
                     /locus_tag="PC472_003638"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Met,seq:cat)
     gene            924..1000
                     /locus_tag="PC472_003639"
     tRNA            924..1000
                     /locus_tag="PC472_003639"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:958..960,aa:Ile,seq:cat)
     gene            1018..1094
                     /locus_tag="PC472_003640"
     tRNA            1018..1094
                     /locus_tag="PC472_003640"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1052..1054,aa:His,seq:gtg)
     gene            1103..1178
                     /locus_tag="PC472_003641"
     tRNA            1103..1178
                     /locus_tag="PC472_003641"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Lys,seq:ttt)
     gene            1186..1259
                     /locus_tag="PC472_003642"
     tRNA            1186..1259
                     /locus_tag="PC472_003642"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1218..1220,aa:Cys,seq:gca)
     gene            1267..1343
                     /locus_tag="PC472_003643"
     tRNA            1267..1343
                     /locus_tag="PC472_003643"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Arg,seq:acg)
     gene            1356..1431
                     /locus_tag="PC472_003644"
     tRNA            1356..1431
                     /locus_tag="PC472_003644"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:Val,seq:tac)
     gene            1507..1920
                     /locus_tag="PC472_003645"
     CDS             1507..1920
                     /locus_tag="PC472_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728923.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2229..5660
                     /locus_tag="PC472_003646"
     CDS             2229..5660
                     /locus_tag="PC472_003646"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2728924.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5902..7830
                     /locus_tag="PC472_003647"
     CDS             5902..7830
                     /locus_tag="PC472_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2728925.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3137 a         1018 c         1595 g         2245 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatatt ggggattagc caagtcggta aggcacatga ctttgactca
      541 tgtatgcgta ggttcgagtc ctgcatcccc agccaaacag tatggtctat tagctcagtc
      601 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
      661 tggagaggtg tccgagtggt ttaaggagct ggtcttgaaa accagtgact tcgaaagggg
      721 ccgtgggttc gaatcccacc ctctccgcca aatgcccaga tagctcagtc ggtagagcag
      781 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccaa aatgtggcgg
      841 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      901 ttaccgctac taaaatttaa tgtgggccat tagctcagtt ggttagagcg cccggctcat
      961 aaccggtagg tctggggttc gagtccctga tggcccacca cattaaatta acttatggtg
     1021 ggtatagctc agttggttag agcgccagat tgtggctctg gaggtcgtga gttcgactct
     1081 cattatccac cccaacttat atggtctatt agctcagtcg gtagagcacc tgacttttaa
     1141 tcagggtgtc ccgcgttcga gtcgcggata gatcaccaaa aaggcggcga catagccaag
     1201 tggtaaggca gtggactgca actccttgat ccccagttcg aatctgggtg tcgcctccaa
     1261 taaaatgcgc tcatagctca actggataga gtgtctgact acgaatcaga aggttagggg
     1321 ttcgagtccc tttgggcgca ccatatgaat agcgagggat tatagctcag ctgggagagc
     1381 acctgcctta caagcagggg gtcacaggtt cgagccctgt taatcccacc agtgcaaacc
     1441 tctagtaaaa ctactagagg ttttattttt gattgaaaaa atttttatat ttaggaggta
     1501 aaaaatttgt atatagtaat aggaaatgaa atagttgata gtgaagaact taaaataatt
     1561 atagataaaa atagtgaatt taaagttgaa aaagatttaa gtaaaagcac aaaaagagaa
     1621 gatgtaatag catatcagtt aagtatagat ttaaattacc tagattctct tataaatgag
     1681 caatgtaatt tagccagtct atctgatgaa gagaaatttg atgaatatat gactttgtct
     1741 gatgaacttg cactggattt agaagagtta atgccaaaat atacaataat aaatgctaga
     1801 gcatataaat tagacgaggt atatggaatt gtaaaaataa tactagctgt agcgtatgca
     1861 gatttagggc acttaaaact ttctgatgta gtaaagaggc tatctagaca agtagattag
     1921 gatatgaata tgaactatat taaaaaaatg tataaaatct tacgtaaaga tgaaaataat
     1981 tatgtaaaca tagaaaaaaa cagtataaaa tcaagtcaat tgtagaaatt ttcacataaa
     2041 ttcatctgaa attctataaa aaatgcctta attttaatga ggtaaatcga gtttacacag
     2101 tctaaaaatt acttatattt ataaatttat cttattaatt aaggaacttt cttaaacatc
     2161 ttcaaaaata aagtatttta gtctataatc tttaatatta cacaaacaat aaaggagaag
     2221 aatagagtat gcttaaaaag tttaataaaa tactagtggc taatagaggc gaaatagcta
     2281 taagaatatt tagagcctgt tcagaattgg ggataaaaag tgttggtata tacagtaagg
     2341 aagataagta tggattgttt agaacaaaag cggatgaatc atatttgata ggagaaggta
     2401 aagggccaat agatgcatat ttagatatgg atggaataat agacctagca aagagaaaaa
     2461 aagttgatgc tatacatcca ggatatggtt ttctagcgga gaatgcagaa tttgctcgta
     2521 aatgtgaaga gaatggaatt acttttatag gaccatcatc taaggtaatg aatatgatgg
     2581 gggataagat taactctaaa aaaatcgcaa aagaagttaa tgttcaaact ataccaggag
     2641 tagaaaaagc aataagaagt actgaagaag caaaggaagt tgcaaacaaa ataggatatc
     2701 ctgttatgat aaaagcatct aatggcggtg gcggtagagg tatgagaatt gtacatcgcg
     2761 aagaagactt agagcttgaa tatgaaactg cttgtagtga atcaagaaaa gcttttggag
     2821 aagatattat atttatagaa aaatatatag ctgatccaaa acatatagaa gttcaaatat
     2881 taggtgataa ttatggaaat atagttcact tatatgaaag agattgttca gttcaaagaa
     2941 gacatcaaaa aataatagaa tatgcaccag ctttttcgtt agatgataaa gtaagaaaag
     3001 agatatgtga agatgcagtt aagttatcaa aacatgtggg ttattctaat gcgggaacat
     3061 tagaattttt agtagatgca aatggtgggc attattttat agagatgaac acaagagtac
     3121 aggttgaaca tactgtaaca gagatggtta caggaataga tatagttcaa agtcaaatac
     3181 taatagccca aggttacagc ttagatagtg aagaaattaa tataaaatca caagatgatg
     3241 tggaaataag aggttactct atccaatgta gaataactac agaagaccct aagaataaat
     3301 tcatgcctga tacaggtaaa attcaagttt atagaactgg ttcaggattt ggtataagac
     3361 tagatggagg aaatggattt acaggggcaa acataagtcc tcattatgat agtttattgg
     3421 ttaagacaat atcttgggat agaacattcc aaggagcaat aaataaaact ataagatcta
     3481 taaaagaact tagagttaga ggtgttaaaa ctaatgtagg attcctagtt aatgttttaa
     3541 ataatcctat attctcaaat ggtaaatgta gtactaagtt tatagatgaa aatccagatt
     3601 tatttgaaat aacagaaagt aaagacagag gaacaaaatt acttcaattt ataggagatg
     3661 taattgtaaa tgataatgct tgtaaagaaa aaccattatt tgatgcatta catgacccaa
     3721 gaatggataa ggatggttca aaatcagaag gaagtaaaat acttttcgat aagttaggta
     3781 aatctgcata tatagaaaag ataaaaaatg ataagaaatt actattaaca gatacaacaa
     3841 tgagagatgc acatcaatca ttattagcaa caagaataag aacatatgac ttattaaaag
     3901 ctgctaagcc aacagaaaaa tatcaaaagg atttattctc acttgaaatg tggggaggag
     3961 ctacttatga tgttgcatat agatttttaa aagaatctcc ttggcgtaga ttacaaaagc
     4021 taagagaaga aataccaagc ataatgttcc aaatgttgct tagagcatca aatggagttg
     4081 gatataaaaa ctatccagat aatgtaatag aagaatttac aaaagaatct gctagacaag
     4141 gtatagatgt atttagaata tttgattcat taaactgggt tgaaaatatg aaaccatcta
     4201 ttaatacagc tttagaaact ggaaaaattg ttgaagcaac tatgtgttat acaggtgata
     4261 tactagataa gactaaaact aaatacaatc tagaatatta tattaaaatg gcacaagaat
     4321 tagaaagctt gggagcagat ataatagcaa taaaagatat gtcaggtctt cttaaaccat
     4381 attcagctta cacactagtt aaagagttaa agaaaaatgt aaaagcacca atacatttac
     4441 acactcatga tacaagtgga aatggtgtag caacttgttt aatggcgtca gaagctggag
     4501 tagacataat tgatgctgca ttagaatcta tggcaggact tacaagtcag ccatcactta
     4561 atgctatagt agaggcactt aagaatacag agagagatac aggaatagat ttatttggat
     4621 atgatgagtt aggaaaatat tataaggatt taagaaaagt ttataataaa tttgaaagtg
     4681 atttgactaa ttcttgtgca gaaatatata attttgaaat accaggaggc caatatacaa
     4741 acttaaaacc tcaagctgat agtttaggat tagttaatag atttgatgaa gttaaagaaa
     4801 aatataaaga agctaatgaa gttgtaggag atataataaa agtaactcca tcttcaaaag
     4861 ttgtaggaga cttagctatc tttatgacta aaaataaatt agataaagat aatataatag
     4921 aagaaggtaa aaatttatca ttcccagatt ctgttgtgga ttactgtaaa ggtatgattg
     4981 gtcaaccaga aggtggaata ccaaaagatt tacaagaggt agttctaaaa ggtgaagaag
     5041 ctataacagt aagaccaggt tcattattac cagcagaaga ttttgatgaa atagctaaat
     5101 atctaaatga aaagtatgat ataaatgcta atataagaaa tgttataagt tatgctttat
     5161 atccaaaagt atatgaagat tatatcaaac atctacaaca ttataatgat atatctaaac
     5221 ttgaaagtga tgtattcttc tatggattaa ataaaaatga agaatgtgaa gtagaaatag
     5281 aagaaggtaa ggtattaaca ataagattag ttgaaattgg tgaagttaag gaaaatggat
     5341 ttagaactat aggatttgag ttaaatggaa tggttagaga agtagaaata aaagataaga
     5401 atttctctgg aaaaattaat aatgttgaaa aagctgatat gaatgaccct cttcaaattg
     5461 gagcaagcat accaggaaaa gttataaaaa taatggttaa agaagaagat gaagtaaaag
     5521 caaatcaacc attaatcgtt atagaggcta tgaagatgga aactataata gttgctaaga
     5581 ctgatggagt aattaagtca atcaaagtaa aagaagatga tatggttgaa gataaacaat
     5641 tattaatgat aatgaagtaa tttaattaaa ataaattaaa aagcactctg aaatgtttaa
     5701 taaaaaatgt tttagagtgt ttttatttat tttaattaaa tatttgttaa aaaaaagagg
     5761 acaattacct ccagatgtag aaataaagct agtaaggaaa atgttatcta aaaattctaa
     5821 atatagtaaa tattttccat aatatttctt aggaaataac agcaaagata aaatacataa
     5881 atttaaaagg tgggtgctta tatgaaggtt tatgatagta aaatgttgag aaatgtagcg
     5941 gtattaggac atagcggatg tggaaaaaca aatctaatcg aaactatagc ttacacagca
     6001 aacactaata agatacctaa attaacagac aaggtaaata tgacttatag catgggatta
     6061 atacctatag agtataatga ttataagttt aacttattgg ataccccagg atattttgac
     6121 tttagcggag acgttgtgtc atctcttaga gcaagtgatg cagcaataat agtaatagat
     6181 gcaacagcgc ctattcaagt tggtacagag aaatcattag aactaactga gagtattcct
     6241 aagataatgt ttataaataa aatagataat gaaaaggcaa gatacaaaga tgcaatagca
     6301 atgttaagag aaaaatacaa taataagata gttcctatga taagtcctat atacaaagat
     6361 aaaaactttg taaagttaca taatgttttt gaaaacatag atgatttaga aggtgaattt
     6421 aaggaacaag ctatgagtgt taaagaagca ttaatggaac ttatagcaga aactgatgac
     6481 caaatattag ataaatattt taatggagaa gaactaacta cagaagagat acaaaaaggt
     6541 attattatag gtatacaaag aggtgatatt atacctgtaa tatgtggttc aactataaat
     6601 aatataggca caaaggaaat attagataca atatcaagtt atttagaacc tatctttaca
     6661 gaggaaagca aaccatttag gggattagta tttaaaacta tggttgaccc atttgttgga
     6721 aagatgtcat atataaaaat tactgagggt gtactttcta aggataaaga tgtttttaat
     6781 ataaataaaa atgtaaaaga aaaaatagct aatatatata cattgagaaa tagtgaatta
     6841 gtagaaatag aaaaagctaa agctggtgat atagtagtta taactaaagt taattctcta
     6901 aaaacaggag acactatatc tgcagataaa gatgcagaag ctttagaaaa gatagatttt
     6961 cctaaaccac aaatatatta tgcagtaact cctaagaata aaggtgatga agaaaaagta
     7021 gcatctgtat taaataaact agtagaagag gaccctacat tacattggta tagaaataca
     7081 gagacaaaac aagcactttt aggaggtcaa ggagaacttc atataaaaac aattaaaaat
     7141 aaaatgaaag ataagtttgg tgttgatgtt gaattgaatg accttaaagt tccttataga
     7201 gaaactataa aaggaacagc tgatgtacaa ggaaaacata aaaagcaatc aggtggtcat
     7261 ggtcaatatg gagacgtaaa aataagattt gaaagatgtg aatctgattt tgaatttaca
     7321 gaagaaatat ttggtggttc tgtaccaaaa caatatatac cagcagttga aaaaggttta
     7381 aaagactcta tgcaaaaagg tatattagca ggatatcctg ttacaaacat aaaagctacg
     7441 ctttatgatg gttcatacca tgatgtagac tcttctgaaa tggcctttaa aatggcagca
     7501 agtgcagcct ttaaaaaagg tatggaagaa gcacacccaa tactactaga acctataatg
     7561 aaacttaaga taactgttcc agaagaatat atgggagatg tcatgggtga tataaataag
     7621 agaagaggaa aaatatttgg aatggaacca gatgacaaag ggaaacaaat aatatttgca
     7681 gaagctccac aagcagagac atttaaatat gcaatagatt taagagcaat gacacaaggt
     7741 agaggatact ttgaaatgga attagagaga tatggagagg ttccatcaca gtttgcagaa
     7801 aaaataattg ggctagctac tgcaaagtaa tgaacaaaat aaatagaaaa ataattaata
     7861 tatattatta aaaatatttt tagggtttta actaggtata ggtttatgtt aggtaaactt
     7921 atacctagtt tttatacatt tttgatgaat aaaaaaatat ataatacttt cttaaatatt
     7981 ttaataaaaa gaaag
//
LOCUS       DAMJYC010000146         7944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJYC010000146 DAMJYC010000000
VERSION     DAMJYC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.152"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            184..1635
                     /locus_tag="PC472_003648"
     CDS             184..1635
                     /locus_tag="PC472_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2728926.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1833..2474)
                     /locus_tag="PC472_003649"
     CDS             complement(1833..2474)
                     /locus_tag="PC472_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2728927.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2948..4327
                     /locus_tag="PC472_003650"
     CDS             2948..4327
                     /locus_tag="PC472_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2728928.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFTNLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4371..5105
                     /locus_tag="PC472_003651"
     CDS             4371..5105
                     /locus_tag="PC472_003651"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2728929.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5194..6393
                     /locus_tag="PC472_003652"
     CDS             5194..6393
                     /locus_tag="PC472_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2728930.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAKKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6417..6899
                     /locus_tag="PC472_003653"
     CDS             6417..6899
                     /locus_tag="PC472_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2728931.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7217..7814)
                     /locus_tag="PC472_003654"
                     /pseudo
     CDS             complement(7217..7814)
                     /locus_tag="PC472_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2943 a          914 c         1353 g         2734 t
ORIGIN      
        1 aaaaaaaggt ttgatttgaa tataaaactg caatatatta aaaaaattat tcattttaaa
       61 ccttgtaatt tgattttata aatattataa ttaaattaat gaatttttaa caaggaatta
      121 ttcatttttt tgattttaag atttattgag aaaacaaaac taaatagggg ggtaattaat
      181 ataatggatt ttttcaatgg tattttaacg agtattaaca atgcagtagt atttgtaaac
      241 aattacttat ggtcgtatat cttaattata atgttagttg taattggtat ttactttact
      301 attaaaacaa attttgtaca attcagatat tttatagaaa tgtttagact tctgggtgat
      361 ggtacagcga ataaaaatgc aaaaaaggaa ggaaaaatct cttcatttca agcattttgt
      421 ataagtacat cttcaagagt tggaactgga aatatagctg gaatagcaat tgcagtggta
      481 gcaggaggac ctggtgctgt attttggatg tggcttatag cattaattgg ttctgcatca
      541 agctttgttg agagtacatt agctcagatt tacaaagtga aagatggtca agcttttaga
      601 ggtggaccag cgtattatat ggaacaaggt ctaaataaaa aatggatggg tattttattc
      661 tcaatactta taactatatg ttatggtttt gtatttaatg cagttcaagc aaatactgtt
      721 tcacttgcat ttaataatgc atttggaata agtaaaatgg ctatgggaat aggtcttgga
      781 attttaactg caatagttat ttttggtgga gtgcatagag ttgcaaaagt ttctgaaata
      841 atagttcctg tatttgcagg attatatata ttagttgctt tggctatagt tgtaatgaat
      901 ataacagaga taccaagtgt tatagcattg atagttgaaa gtgcatttga ttttaaagga
      961 atggcgattg gtacatttat gggagttgta atgactggag tgaagagagg tctattttct
     1021 aatgaagcag gtatgggtag tgctcctaat gctgctgcaa cagcacatgt aactcatcct
     1081 gtaaaacaag gtttaattca atctttaggt gtttttacag atactataat aatatgtagt
     1141 tgtacagcat tcatggtatt attatattca ggttatagta gtagtggagc aactggaatt
     1201 gaattagctc aagaagcttt aactcatcat ataggaccag ttggaaatat atttatagca
     1261 gtttgtatat tcttgtttgc atttagttct atagttggta attactatta tggtgaatca
     1321 aatatggagt ttatgagtgg aagtaagact aaacttaatg tatttagagt ttttgttgta
     1381 ggtatggttt tatttggttc acttactcaa gtagatgtag tttggaatct tgcagactta
     1441 tttatggcat tgatggcgat aataaactta atagcaatag cacttttagg aaaatatgca
     1501 tttattgctt taaaagatta tacaagtcaa aaaaaatcag gaataaaaga ccctgtgttt
     1561 attgcagatg aaatagaagg cctagaaaag gtatcagagt ggcataggga attatctgag
     1621 gaacatttag gataaaaaat agtataaaat tataaaacca ttttgaaaga ctagagtatg
     1681 tatatacttt atatgaatat aatattgaat taatttatat tactggaatt tttaattcca
     1741 gtaatatttt tttaattaca aggatattat gatgacttaa agactgtgga aaactctttt
     1801 ttagttttca ttattaatat taattgacaa atttagtttt tatttaaatt tcttaagtgc
     1861 atataaatcg tattttttga taatcccatt aagtctgcaa ctttaacaac agaatcctta
     1921 attttaaaaa taccactttc atttaaaata gaaacaatag atttattttt atcattgcta
     1981 gatatattag aatcattttc aacaattacc tttgcatctt ggtatgattt taaaattact
     2041 tcatcagtat cttctacgat attttctaaa acaggagcac tttgcatagc attatgttcc
     2101 ggaatatatg aaccaattat tcttgttagt ggtgtattaa gataaaaatg tatgcagagt
     2161 agaccgatta ttttattatc aactcccctt atcactatat ctgttgattt taaaggttca
     2221 cctagtttgt ttctattaaa ataactgata aaatctccat cagcatcatt ttcgagttta
     2281 ttaatgagtg ccaaatcaaa atcagttata atatctcctt cttttacagt attatattta
     2341 ccattagcta ttttatttat tgaatagtgt actccattaa atttatataa aataaactcg
     2401 tatccttctc ctaaatacgc agataatcca tccactagat tgttataaga atcgaaaata
     2461 tagttatcag tcatgttaaa agtcatatgt tacctcctaa gaaatatctt aatatcatat
     2521 taacatataa aatgaattta ataaaatata tttcagtgaa ataataaata taatgaaaaa
     2581 aaaactattg actgaaaaat atttcagtga tattattaaa atacagtaag gcaaacgtat
     2641 tattgacaga atatataaaa tgttaaaaat agtatctagg aattaaacaa attaaaggac
     2701 tagtgatttg aattttttta gtataatatg tgacttccaa ttaatattta ttaaacacca
     2761 ataaaattgt aaatatgctt aatgatatac tttagcgatg aagtgaaaat attttatttt
     2821 tatgattaac ctataggaat taatttagca tactgtttag ctagtaaatc taaataaatt
     2881 ttagctttag ctgatatcat gttgaataaa gtttctattg gattttgtat aacaaaggag
     2941 tggggagatg gcacaagata aaattgtggt tgaaatttta gaaaacattg gtggtaagga
     3001 aaatattaag gaagtagcac attgtatgac tagactgaga ataaaggtat gtgatagttc
     3061 tcaggtaaat caggaaaaac taaagaaaat aaaaggtgta ctaggtcttg tattaaaagg
     3121 tgagcaatat caaattgtta taggtcctaa tgtatcacaa gtgtataata ctcttaatga
     3181 agttatagga attatacctg agaaggatag taaaaaaaca atatctgaag gtaaacaaaa
     3241 tagtattgca aaaatattag atactattgc aggtattttt aatcctatag ttccagcatt
     3301 agcgggtgca ggtctaataa aagctatatt agcattacta gtagcatcaa agtttttatc
     3361 tgcagatagt gaaacctata taatattaaa tacaatatct gatggtgtat tttcattttt
     3421 accatttatg ttagcttact cagcagctaa aatatttaaa atgaatactt atgttgctgt
     3481 agcaatagca ggagcaatgc tacatcctaa tttcactaat ttaattcaac aaggcttaac
     3541 taaaatatct tttatcggta ttccgatgtc actaatccag tataacggaa gtgtatttcc
     3601 gatagtacta actatttggt ttgcatcgta tatagaaaaa ggagtagata aaataacacc
     3661 aaaggcactt aagataatat tagtacctgc attgactatt ttatttgcag ctcctatagc
     3721 attaattatt attggaccaa tagctcaaat gctaggaaac tatatagccg gtggagcaag
     3781 tatcctattt gaaagaggag gaatatttgc agggttaatt tacggaggaa tttattcatt
     3841 catggtagta acaggattac atcatggtat ggttcctgta ttagtagatg gaataacaag
     3901 atatggattt aactatataa gtcctgtgtc aggtagttca aatatggctc aagcaggatc
     3961 tgcatttgga gtatggttaa aagcaaaaga taaagatttg aaggctgtag cagctacagc
     4021 taccatatca gcattaactg gagttaccga acctgcaata tatggtgtta atttaagatt
     4081 aaagaaaccg tttttatgcg ctgctatagg tggtgcagca ggaggtggat ttgctgcata
     4141 tttccaatgt aaagcatttg ctatgggagg tccatcgttt ttaactcttc ctatgtttat
     4201 aggagaaggt ggacatgtag gattggtagc aatagcattt acaatagcat ttatagtatc
     4261 tgcaatagct acctatatag taggatttga agaagaggta acagcagatt cacacgctaa
     4321 cttataataa agagaaatta tttaacatat caaaaaggag attaattact atgaaaattt
     4381 atttaactaa agattatgaa gaaatgagcc aagtggcagc acaaatggtg ttaaagtata
     4441 tgtacagcaa tgataataaa agaataaacc tttctataac agcaggtcaa acaccaaaaa
     4501 gaacatacga aatattaagt gaatttgtta agaataaacc ttattttgaa aatgtacatt
     4561 attataactt tgatgaaata ccaataaaag gcgaaaaagt tggagttact ataagaaatt
     4621 taactaatat gtactttaga gaagctcaaa taccagaaga aagaatacat gtgttagatg
     4681 aaaataatta tcaaggcttt gatagaaaga tagaagccga tggtggaata gacatggtat
     4741 taatgggatt aggttgggat ggacacttct gtggaaatct tagtggtaca gtcaactctt
     4801 ttgatgaaga gacaagagct gtttcaactt atataacaga tgatatggct aaactattaa
     4861 caaaatctgt aggtggaaat ccagaaaaat tatttgatta ctatgtgaca tatggaccta
     4921 aaacaataat gcattgtaaa aatataataa tgatagtaaa cggaaaacac aaagctcaaa
     4981 tattaaagca aacactattt ggaccattag atttattatg tccatcatct ttattgagat
     5041 tacatcctaa cttcacatta atagtagatg aagaagcagc atctgaaata aaagatttaa
     5101 tataaataag taaggaatta aaattaaaaa tgaatttagg tgcaaagtaa tattttattt
     5161 tgcacctttg ctttaataaa aaggagaata aaaatgtata atcatatgaa aaaagtattc
     5221 caaggattag agggtgggtt attttcaagt gtagagaaag ctgatgtagg cgattcttat
     5281 caaaaattag gtgagcaggg tgtaagttta atgggatggg cagatccatt tttcccagat
     5341 tactgtatac ctagacatat attagatgca gctacattag cgatatcaag taatatgtca
     5401 actcattata cagcgcctgt aggtaatgac ttattaaaaa ttgagatagc aaaaaaatta
     5461 aaagaagtaa ataatttaga tgttgatttt aaaagaaata tattaataac tcctgggtca
     5521 gactcagggc tttattactc aatgttaccg tttataaatg aaggggatga ggttcttata
     5581 ccaacaccat gctatccaaa taatatacaa aatactaaat taatgggagg agttcctata
     5641 tttgtagagt taaaggaatc agatggatat caaatagata taacagaatt tgagaagaga
     5701 ttaactaata agacgaagtt agtagttctt actcatccta ataatccaac aactacaata
     5761 tttaataaag aatctttaga aaaattagct aaatttatta tagacaatga tttaatatta
     5821 atatgtgatc aagcatttga agattttact tttgaaaatg aattaataac accagcttca
     5881 ttagaaggta tgtttgaaag aacagtaaca atattttcaa catcaaaagg tatgggatta
     5941 agtggattta gagttgcata tatggtatgc agcgataaat taatggacaa atactatgga
     6001 gcagcagtga cagtattagg tgctactaat acagtagctc aaatgtcaac gattgaagca
     6061 tttaaagata gaagctttat ggaggatttt gaaagagttt ttgataaaag aaggcaagaa
     6121 gcatataaga taatcaatga aatacctaat gtatctatgc agatgcctga gtccggattt
     6181 ttaggttgga taaatgtatc aaagcttgga acatcatcag aaatacaaaa gtatctagtt
     6241 gacgaggcta aagtttgtat taatgatgga attaattatg gtccaggagg agaagggcat
     6301 ttgagaataa ttttaggaag ctataaagac gatagtaagg taattggtgc attaaataga
     6361 ataaaagatg ctttaattaa aaagcaacaa taaataaaaa ataattggag tggagtatgt
     6421 tatcaatatt taagagtaaa aaaaatgaca acaagcttaa ggcatttgca tcagggaaag
     6481 taattcctat agaagatgtt gatgataatg ttttttcatc taaaatgtta ggtgatggaa
     6541 tcgctattta tccaactaat gaatatgttg tagccccttg tgatgcagaa gtaaatataa
     6601 taatgaaaga tactaaacat gcagttggaa ttaaattatt taatggcatg gaaatcttat
     6661 ttcatgttgg aatagatact gtatctatga atggagaagg ttttgagata tttgtttcac
     6721 caggtgacaa gataaaaaga ggtgataaat taatatcttt taatagtaaa ttaataaaag
     6781 aagaagggtt acaagatgta gttataatgg tagttactaa ttcagaacag tataaaaata
     6841 taaaatatca tattggactg gaggcagtaa tcgatgatac tattattgca gaaatttaaa
     6901 aacagttatt taagtaaata cttagataat ttagatatag ttaaaattaa atccttaaaa
     6961 tcaaattatt tagtttttgt gtaattttaa ctataaagcc gattaataat ataaagtata
     7021 ctaaataata tggttattat gattgttact gacaaagcct aagtagatat aacaatttgc
     7081 tctaattgag tattgctata tctacttgtt tttatattta aaataaaaga gtctgcattg
     7141 aaaagataat tctgatgaga gtaaataggg tatttaataa tagtttaatt ctgacttatc
     7201 aagatgaacc tattcatata cctaaaacat ctttaacacc ttctaaattc atacaaatag
     7261 ctgtataggc tgccttattg attatttttc cgtcaatttt tactttgtag tgaacagcat
     7321 atataaatat tatcgaatag attgtatcaa gagctctatt ttgctattca ttagctaaag
     7381 atagtactct gtcagttatt tttgaaacta atgcatgaga tatatcaatt ccgtaaatat
     7441 tatttaaatg tatgatatat atcgcgagtc gacattcctt ttgagtacat gccaattact
     7501 tggttctcta ttccagaaat ttcattttcg taattttgaa taaccctagg ctggaaatca
     7561 tccctaggga tatttaattt tacctctcca aaatctgatt taacattctt agctctatat
     7621 ccatttcttg aatttgatgt tgctttactt tggttatcat atctctcgta cattaaatga
     7681 tcttcaatct ctggctctaa cattttgtga attgtgcttg cgaataaatc ttttaacatc
     7741 tcttggatgt ctttagtatt ttttacatca tactcttgaa ctaagtgttt taatatttaa
     7801 tttttgttgt acataaaaaa tgtgtacctc ctattttggt aattagattt aatatcttga
     7861 cttttaggag aatacacatt ttattttaca gaccctcttt tctagaaaag tttttatgaa
     7921 aattgtgata taaattatta ataa
//
LOCUS       DAMJYC010000147         7943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJYC010000147 DAMJYC010000000
VERSION     DAMJYC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.153"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            178..807
                     /locus_tag="PC472_003655"
     CDS             178..807
                     /locus_tag="PC472_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2728932.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(921..2435)
                     /gene="glpK"
                     /locus_tag="PC472_003656"
     CDS             complement(921..2435)
                     /gene="glpK"
                     /locus_tag="PC472_003656"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2728933.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVDDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2679..3221
                     /locus_tag="PC472_003657"
     CDS             2679..3221
                     /locus_tag="PC472_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728934.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3235..5727
                     /locus_tag="PC472_003658"
     CDS             3235..5727
                     /locus_tag="PC472_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2728935.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6148..6546
                     /locus_tag="PC472_003659"
     CDS             6148..6546
                     /locus_tag="PC472_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728936.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6763..6972)
                     /locus_tag="PC472_003660"
     CDS             complement(6763..6972)
                     /locus_tag="PC472_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728937.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(7004..7213)
                     /locus_tag="PC472_003661"
     CDS             complement(7004..7213)
                     /locus_tag="PC472_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728938.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7512..7931
                     /locus_tag="PC472_003662"
     CDS             7512..7931
                     /locus_tag="PC472_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728939.1"
                     /translation="MDTLGERIVYLRKSKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPFNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
BASE COUNT         2984 a         1149 c         1036 g         2774 t
ORIGIN      
        1 ttctttatat agttttaagt ataataaaat catgtatact aaatgcatgt taaaatatta
       61 ttttaaaaca tgcacaatat ttgtacatat tgtagacata taaacctata aattatacaa
      121 tagtattgtt aagattatat atttttttaa cataaatttt gtattggggg aaaaacaatg
      181 gataaaaact atacgatgcc aatataccaa aaaatagcgt tggatattgc aaataaaata
      241 tacacgggtg aaatacaaga ggattcagta ctatttggac gctctgtttt ggctggtaaa
      301 tacaatgtat ctccagaaac gataagaaga gctgtcaaaa ttttagagga cattggcgtt
      361 gttaaaagca taaagggtaa aggtgttatt gtactatctc cagacaaagc ttcttctttt
      421 ataaaaaaat atagagatat tacaaatata tcatcatata aatcaacact ttataattta
      481 atagatacta aatctaattt agaaaatgaa atattagata ctataaataa aattttagac
      541 tattctaaca gacttgaaat aatcaatcct ttagtgcccg ttcagttcac cataaattct
      601 aattgcaaat atattggtca aacagctgca caaactaaat tttggcaaaa tacaggtgct
      661 acaatagttg caattaaaag aggtgaagaa cttataatat ctcctggacc atatattgaa
      721 tttttagaag gagacattct cttagtagtt ggtgaccaac acatttacaa ttctatacca
      781 atgtttttat atgaaaacga aaaataaatt atataattta gtatgattat ataaaaatgc
      841 aaaattaaca acaaaaaaag agcttcatat tttacataaa gctcttttta aattcaaata
      901 aatttaattt tgattaattt ttaaatatcc aattcatctt ctttttccca gcttaaagct
      961 ctcgatacag cttttttcca tcctttatat aatttacatc tcttctcttc actcatattt
     1021 ggattaaact ctttttctac tgaccacttt gaagtaattt catttcttcc aatataaaat
     1081 ccaacagaca accctgctaa ataagcagct cctaaagctg tagtttctac tattttgggt
     1141 ctatctatat ctacatttaa tatatctgat tgaaattgca ttaaaaaatt attattactt
     1201 gctccaccat caacttttaa tgattttagt tttaattttg aatcgttttg cattgcttct
     1261 aaaacatcct ttgtctgata agcaattgat tccaatgtgg ctcttactat atgttctttt
     1321 tttgctcctc ttgtaagtcc taaaattgaa cccctagcat acatatccca atatggagct
     1381 ccaagacctg taaatgctgg cactacatat acaccattag tatcatctac cctatttgca
     1441 tacatttcac tatcttttgc agattcaatt atctttaatt catctctaag ccattgaaca
     1501 gatgctcctc caataaatat acttccttct aatgcatatg ttacttttcc atcaactccc
     1561 caagcaattg ttgtaagtaa cccatgctta gatttaacta tgttactgcc tgtattcata
     1621 agcaagaaac aacccgttcc ataagtattt tttgcagtac cttcttcaac acaattttga
     1681 ccaaataatg ctgcttgttg gtctccagca catcccgcaa taggtatttt agctcctgat
     1741 aacatgcgct catctgtatg accataaaca tagctagaag gttttactaa tggcaacatg
     1801 cttattggta tgtccaattc tcgtaatata tcctcatccc attctaaatc atttatattg
     1861 tacatcattg ttctagatgc atttgagtaa tctgttacat gtacttttcc acgagttaaa
     1921 ttccatatta accatgtatc tatagttcca aataataact ctccttgttc tgctttttcc
     1981 ctagctcctt ctacattatc taatatccat tttacttttg ttgctgaaaa gtatgcatct
     2041 attaaaagac ctgttttttc ttttattgat tcttccaatc ctttattttt aagttcatca
     2101 catatttctg acgttctcct acactgccat actattgcgt tataaatagg tttaccagta
     2161 tacctactcc aaacaaccgt agtctctctt tgattagtta tccctattga agcaatttct
     2221 tctggtctta tacctgctgt ttctataact tctctcatta ccccactttg tgacccccat
     2281 atttccatag ggtcatgctc tacccatcca gcttttggat aaatttgatt gaactctttt
     2341 tgtgcaattt tcactatttc gcctttttta ttaaatagta tagctcttga actggttgtt
     2401 ccttggtcta atgccattac atacttcttt tccatcacca tattctcctt atcatataat
     2461 atttaattta ttaaaaagaa aaatttaata tcacatattt caataaataa tcatacttaa
     2521 gtacaaattt aatataatag atatttagta agaagacact tattttctat catatctata
     2581 taataatatt atacattata gcttacatat tgattaataa attttaaagg agtataataa
     2641 acagtgttca taataatttt aataaaagag gtggtaatat gccaaagatt ttagaaaatg
     2701 taaaagaaga tatattaaaa gtgtctcggg atatgatttt agaagaagat tactcgaata
     2761 ttagcataag gaaaatagct caaaggtgtg gtataagtgc aggtacagtg tataattact
     2821 ttaattcaaa acaagaaata atagaatata tcacaaaatc tgaatgggat ttattgataa
     2881 ggcgaattga atactccaat aaaaacactg aagattacat aaaaaaacta aacattattt
     2941 ttactgaaat aagaaatttt ataaacaaag ttcataatat ccaatacaat gactttttaa
     3001 acacttttga aacagagaga ttttttgaaa tgaaaaaaca caaagacgac tttcatgaac
     3061 agctttcaaa taaagtatat gaagctttag aaaaagaaag tttttttaat aatgacaaat
     3121 tagtatgcaa tatcataata aaaatgtttt tttcatattc aacatatctt catatagaat
     3181 ttgaagattt aaaaccctat attgaaaaat taatacatta aggtggtgtt taccttgaat
     3241 cataatacta gtattaagaa caaggacagt aatccaaaaa tatttttagt acttacactt
     3301 tatctattag gtatatttat gggagctatt gatactggaa tagtatcacc agcaagaacg
     3361 attatacaaa atagccttgg agtaaatgaa aaaacgggaa tttggatgat taccatatac
     3421 acattaacat acgcttctgt aattcctatc tctggaaaat tagcagataa aataggaaga
     3481 aaatatgtgt atctagttag tatatttcta tttggtagtg gttcattaat atgtggatta
     3541 tcatcactat tttccaattt ctatatttta ttaattggtc gtgttattca agcaattggt
     3601 ggtggtggaa tcatgccaat tgctacagca gaatttggaa ctaccttccc agaaaataaa
     3661 agaggcatgg ctttaggttt agttggggca acatatggta ttgccaatat tttaggttca
     3721 agcattggta gcacaattct aagtatcttt ggaactcaaa attggaagtg gctatttttt
     3781 gtaaatcttc caatttgttt aatcatactt attggtggag ttttctgtat taaaaacaat
     3841 aagtctgaat ctaaagaaaa aatagataag cttggtacat tgatgttgat atgtataatc
     3901 gtatcactat tatatggtct tatgaatata gactttttta attttaaaaa ttctatacaa
     3961 gatatatcag tttatccata cttattgaca tttattgtac ttatacctat tttcatattt
     4021 attgaaaata gagctaaaga ccctatttta agctttgaat attttttaaa tccgagaata
     4081 ttgatagtac ttattttatc tttaattgtt ggtattggaa tgatgggtat ggtattcgtt
     4141 ccacagtatg ctgaaaatgc attaaagata aatgctggtt ctgggggata ttttgtagct
     4201 atattgggat tatttgcagg tattgctgct cctttaagcg gaagacttat tgacaagttt
     4261 ggggcaaaaa aaatactatt actaggattt tctgtatcta tgataggctc tctttaccta
     4321 gttttaattg ctctaaaaac caatactgta tttagtgtat gtgtaagttt aatatttatg
     4381 ggattaggaa tgggatttac tatgggaact cctctaaact atatgatgtt atcaaataca
     4441 agattagaag aatctaattc agcacttgca acattatcct tgatacgctc aattggtact
     4501 tcaatttctc cagctataat gattggattt atagcacatg caggattatc tgtgcaagat
     4561 aatattatgg acttagttgg aaagccctca actcctaaaa tagttcaact tgaagaactt
     4621 gaaaacatga tagatgactt aaaatcagac cctgaaatgg caaaaaattt aaaaaatgtc
     4681 tctatcccaa atatgaatga tacttcaaat ataaatatgg acatgacagg tggcaaactt
     4741 ccaaaatcct tacttaataa agttcagtca gcagatacaa ccaacattac caatataaca
     4801 aaagaaatat caacaacaat gtttgatgaa aaagttccat ctataattac taaaatacag
     4861 aaaaatgtac aaaaaggaat agatgggaca caaagtggaa taaacggtat tgaagatggt
     4921 gaaaataaac taaatagtgg tattaaagga gttcaaactg gtattgacaa tatggtaaaa
     4981 gctagagctg gtattaatca aggaatagat ggtgtaaaaa aaggtattgc tggtgttgat
     5041 aaaggtataa aaggtatgga acaaggtctt gaaaaacaag atgaggccat caaggaactt
     5101 acaactgctt ataataaaat acctactaca gaaaaaactc ctactgataa cgaaaataac
     5161 tcaaatgaac aaaaccagag taataccagt aattctgaat cagatgacac taagaatgat
     5221 tctacagaaa ataagaataa caattccagc gagaataaac caaatgtaaa tgaacaaaaa
     5281 gaaagtttaa atgctcaaat acagcaacta aaaaagacaa gagatgactt aaatacaaag
     5341 ttacaaaaaa ataaaactca aaagaacgaa ttaagtaaaa aactaagatc aatggaatct
     5401 caaaagaaag agctacaaag caagttaaat aattctataa gccaaaagaa agacatggaa
     5461 aaaagccttg atacaatgca acagcaaaaa aaatcactcc aagcagtttt agcaaagaca
     5521 caagaggtaa aaactgaaat accaaaggct tttgataaat caaaattgga ttatataaat
     5581 tccatagaaa ataatagaac aaaaatagaa aacacactac aatctactct aaactcagga
     5641 tttaaacaga tgtatataac agtattctgt gtaaatctac tagcatttat aatattattg
     5701 ttctataaag aaaataaaac tagataaaag agtgttatag tattatattg taaatttatc
     5761 ttattcttaa atattttatt ctaaagaata aattcataaa atttttgtaa ttgattaata
     5821 taaattacaa tataatacta tttttctctt aatctttttc tcttttacag acaatcaaaa
     5881 agcaatctta gtttatcgaa ataaatttat acaatctatt catacacccc tttacatttt
     5941 tataacaatt aaacctattg ttatatatac aaatcaaaat aaaccatata ttttcaacta
     6001 taatatgtta aaatcatatt atgagcatat taatttaagc aaaatgtcat ttagacttta
     6061 ttttagtagt tttatattat aaatttatat atttttaatt ttaaatgtca tttagacatt
     6121 actaataaaa agaaaggagt gtattttgtg gatactttag gaaaaagaat tgcttatttg
     6181 agaaactcca aaaaacttac tcagcgcaaa cttatggata ttttaaaatt tgaaaactta
     6241 ggcaagtatg aaacaggaga tagaaaacct aattgtgata tactaatgtc aatagctgat
     6301 tattttaatg ttacgactga ctggcttctc tatggtaaag agaaggtaaa tgtcaatagt
     6361 agtgtaaaag aagataaaga agattactta catgttacta atgatgaaat gacgatatta
     6421 aatctataca gacaactaaa cgaacgtgat aaaataaaaa tagaaggaat acttgaactt
     6481 aagatatctg aatataaaga cttaaaaaag cattcctcta ataataatga agataaaatg
     6541 gtttaaacat acataatttg atacgatttt acatcatcta ttaaccttct ttttttgtag
     6601 tatattacta aaatatggat attggattat ttaaaaacaa gtatagtcta atttcaagca
     6661 catttataat atttagatta ccttatccat attttagttt aagtactttg aaaaatataa
     6721 tacatctcta tgtataactt atattcctaa gaaatgcatt actcatgttt ttaattcttc
     6781 caacatcttt ctatacacaa ttgtacatcc ttcataattc ccctctttaa gaaggattga
     6841 acatgtacta gaatatttag ttaaagtatt taatctttcc atatttaagc tttttatatt
     6901 actgagtgct gtaataaaat catcaattgc tctacaacac tctataaccc catctgaatc
     6961 caatttacac acaagaatat tcacctactt tatatactat ttattaatca tcttctgaat
     7021 attctatatt aagtaattga taaattgatg atttatattt tttacctgtt cttcttccat
     7081 ttaaaatagc tgacaaatag ttttcattaa cacctatttt cttcgctaat tccctttgag
     7141 tcatatttaa ttcaataagt ctttgcttga cttgcactcc caaaaaaggt atccattttt
     7201 ttcgagcatc catttatcaa cttcctttct aagtgattta tatttacttt tatattattt
     7261 tagcttacgc accccctcta tttagtatat atttttaata tgtacacata ttaagtatat
     7321 aaatttattt aatactaaat aagatttttt gattactttg tgctaaaatt aggatgtaaa
     7381 ttcgtatcat taagttaatt ataaagtcat tttgacctta agtcaatggt ttttatgtca
     7441 ttttgacctt taatttgaaa ctatttacat cttaaaaagt ctttttgaca ttaattgaat
     7501 ggagagtgtt tatggatact ttaggagaaa gaattgttta tttgagaaaa tcaaaaaacc
     7561 ttaaacaata tgaactagaa gaaatgttgg gctgtgacaa cttaagtaaa tttgagagaa
     7621 acattagaaa accaaattat gaaatattaa aatctatagc tgagatattt aatgtttcag
     7681 tagactggct attaaatgga gataatctat cacataaatc agatttaatt tgcgattcct
     7741 cctcaaacta tccatttaat agcataaact ctaatgaaat aaagctttta aacaacttta
     7801 gaaagttaaa tgattatgat aaagccaaaa tagaaggtat gattgaatta aaacttcatg
     7861 aatatgaaaa agagaaggat ttaggaaaaa ttgagtataa caaaaataaa gatgagaaaa
     7921 tagataaata atttttatgt tgc
//
LOCUS       DAMJYC010000148         7791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJYC010000148 DAMJYC010000000
VERSION     DAMJYC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.154"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            49..1107
                     /locus_tag="PC472_003663"
     CDS             49..1107
                     /locus_tag="PC472_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2728940.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGSYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1211..2395
                     /locus_tag="PC472_003664"
     CDS             1211..2395
                     /locus_tag="PC472_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2728941.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2602..3315
                     /gene="rpsB"
                     /locus_tag="PC472_003665"
     CDS             2602..3315
                     /gene="rpsB"
                     /locus_tag="PC472_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2728942.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3394..4305
                     /locus_tag="PC472_003666"
     CDS             3394..4305
                     /locus_tag="PC472_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2728943.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4390..5094
                     /locus_tag="PC472_003667"
     CDS             4390..5094
                     /locus_tag="PC472_003667"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2728944.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5120..5677
                     /gene="frr"
                     /locus_tag="PC472_003668"
     CDS             5120..5677
                     /gene="frr"
                     /locus_tag="PC472_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2728945.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5680..5883
                     /locus_tag="PC472_003669"
     CDS             5680..5883
                     /locus_tag="PC472_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728946.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5912..6646
                     /locus_tag="PC472_003670"
     CDS             5912..6646
                     /locus_tag="PC472_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2728947.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6659..7438
                     /locus_tag="PC472_003671"
     CDS             6659..7438
                     /locus_tag="PC472_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2728948.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3208 a          792 c         1463 g         2328 t
ORIGIN      
        1 actaatacaa aatagtaata attgttttgt actggaggat atatatttat gaattgtaaa
       61 tggatatcta aaatagatga gagaaaaaaa tgtcatagag aagcagattc ttctggatat
      121 tgtatctttc ataaagaaaa taagtctgat gaagagatac agttgatgat ggatactctt
      181 cataaagaag agataagtga atttaatgga tttgtttttg aaaatgaatt taatgcggaa
      241 gaaatattaa cttacaatta taaaatactg gatttttctg aatccatatt taaacaaaaa
      301 gcaaatttta aaaaatatat ttttaaaaag aatattattt ttaactatac agaatttaga
      361 gataaagttt tatttaatgg ttgtgtattc ttagaaaact gtgattttaa tagaaccata
      421 tttagtaaac actatataaa tgaccgtata tttgaaaaag tgaaatttaa aggacctgat
      481 ttggtagtta ataaagtaga aaattttcca cgaatggatg gaataatttt tagcatgtgt
      541 acaaaatttg tgttaaagaa tgttgaatat ggaaaaagtg agtatgagca tggcaagata
      601 aattatagaa tagctagaaa tcaagcaaca aaaattggag aatatgagat gataggatct
      661 tactattata aggaaagaat ttatagtagt aaaataatga aacgttctaa ttatcctact
      721 ttcagtgatt atttagttga aaaatttttt gaccaaatag cacgttacac aacaggttat
      781 ggagaaaagc catggaatat attattagtt attatagcaa taataagtgt ttttgcttta
      841 ttatacttat ttgtaggcat agaaagttca aatagcacac ttgtagcttt agatataaac
      901 aatataggtg attactcact aagtgagatt ttcaaaatgt atatggattt atggtatttt
      961 agcatggcta catttagtac agtaggatat ggagatatgg ttgctacaag tttgattggg
     1021 aaagcattgg caggtataga agtatttttt ggagttacaa taggagcaat ttgggcatct
     1081 gtgattatta aaagaatgat aaggtaatat ttgtgtaaat atggataggt attaattact
     1141 agatataaat tggactaaat agggaaaatt taaatataaa tatacttaat aatccaagaa
     1201 ggtgaaaaat ttgaaaaatg cactaaaaaa aattatatca ttagccatga ttctagtttt
     1261 tataatacca aattattcat atgcagatga taaatctgta gacaagtatt caaaaacatc
     1321 tatacttata gaccaagaaa caggtagagt tttatatagt aaagaacctg atatgaaggt
     1381 accattagct agtttaagta agatgatgac ttttttactt gcaatagaag ctattgaaaa
     1441 taaagaagta aaagaaactg ataaagtaaa aatagacaaa tctattgcat cagtaaaagg
     1501 gtcaagttat aaacttaaag atggtgaaga agtgccactt atagagctta tgagaggtct
     1561 tatgattgta tcaggaaatg atgcggcaat tgctatagca aaacatattt gtaaaacgaa
     1621 agaagcattt gtagatagaa tgaataaaaa agcaaaagaa ataggaatga gtaatacaca
     1681 ttttctaaat ccaaatggtc ttccaatata cgatttaagc aaccccaaaa aaccagcaaa
     1741 agaaaatatc tctactgcaa aagatatagc tattcttgga aaatacatgt ttgaccatta
     1801 tgaaaaacaa gtaacagcta taactgatat ggagacatat acaaattcag atagaagttt
     1861 tgaaaaaagc aatacaaatg cattattaag aattatacca gaagtagatg gtataaaaac
     1921 tggatatact ggcaatgctg gatattgctt atcattttct atggttgtaa ataaaaatga
     1981 taaaaatgag aaaaaacgta gagtaatagg ggttgtatta ggtactaacc ataaaaataa
     2041 aagaacctct gcttcattag ctctactgaa atatggaaaa gaaaatttca atatgaaaaa
     2101 agtaattgat aaagatagct ttattggtaa gaaatatata aaaggaatga aagagttaga
     2161 ggttacatta aaagcaaaag atgaacttta tacaatatta aaagatgatg aaagtataaa
     2221 atcagaagtt aagattaaaa atatagagta ccctgttaaa aaaggtgata ctatgggtat
     2281 tataaaatat tatactaatt caggtgactt acttggaagt gtagatatag taagtaataa
     2341 tagtatagat aacgtatcat taaaaacaaa attaaaaatg aaatttgcaa attaaattca
     2401 aaaaatggta taaaatacac aaaaaaatat aaaaatagaa aatatggaaa aaatattgtt
     2461 atttcttcac ctctgtgata tactactaga ggtgaaaaaa taaaataaat cacacacagc
     2521 attgggatcc ttaaaaggtg cctattacat ggttttttag ggagaagatc aagctggagg
     2581 tataaaacca ggaggtaaga tatgtcagta atatcaatga aacaattatt agaagcaggt
     2641 gttcactttg gtcaccaaac aagaagatgg aaccctaaaa tggctaagta tatattcaca
     2701 gagagaaatg gaatatatat aatcgactta caaaaaacag ttaaaaaagt tgaagaagct
     2761 tacaagttca ctaaagaagt agctgaaaca ggaaagccaa tcttattcgt aggaactaaa
     2821 aaacaagctc aagatgcgat aaaagatgaa gctgaaagat gtggaatgta ctttgttaat
     2881 gaaagatggt taggtggaat gttaacaaac cacaaaacta taaaaacaag aataaataaa
     2941 ttaagagaat tagaaaaaat ggaagaagaa ggcgtattta atgttcttcc taaaaaagaa
     3001 gttataaaat taagagctga aaaagaaaaa ctagaaaaat acttaggtgg aataaaagac
     3061 atgcctgaat taccaggtgc aatgttcgta gttgacccaa gaaaagaaaa catagctatt
     3121 caagaagctc atagattagg tataccagta gttggtatag ttgatactaa ctgtgaccca
     3181 gaacaattag actttgctat accaggaaat gatgacgcta taagagccgt aaaattaata
     3241 actggtgcta tggcaacagc tgtaattgaa ggtagacaag gtgctgagga agaagtagct
     3301 gaagaccaag aataataatt tgacaattaa atggtaaggg tcgcccttac catttataat
     3361 atggaataga ataagtagga ggaaacttaa aaaatggcta atataactgc tcaaatggta
     3421 aaagagttaa gagaaagtac aggcgcagga atgatggact gcaagaaagc tttacaagaa
     3481 gctgaaggaa acatggaaaa agcagtagat ttattaagag aaaaaggatt atcaaaagct
     3541 gctaaaaaag ctggtagagt tgctgctgaa ggtctagttg caatagaaat gaatgatgat
     3601 aatacagttg cttctatggt agaagttaac tcagagacag atttcgttgc taaaaacgaa
     3661 gacttcaaag tgttcgttaa agatgctgct tgtatggcat tagctactga taaagaagat
     3721 atagcttctt tattaggtga aactcataaa gagggaataa ctttacaaga agttttaaat
     3781 aatagagttg caaaaatagg tgagaaatta gactttagaa gatttgctaa agttgtaact
     3841 aatggacaag ttgctggata cattcacggt ggtggaaaaa taggtgttct tgttgaaatg
     3901 gaaacagaag ctagagatgc taaagtttta gaattaggta aagatgtagc tatgcaagtt
     3961 gctgctatga atcctaagta tgtttcaaga gatgaagtag atgctgaata catagcacat
     4021 gaaacagaag tattaactca acaagcttta aatgaaggaa aaccagctaa tatagttgaa
     4081 aaaatggtta aaggaagatt agaaaaagaa ttaaaagagg tttgtttatt agagcaaact
     4141 ttcgttaaaa atccagatat aactgttaaa caattagttg ctgatgttgc taaggcagta
     4201 ggttctgata taaaagttgt taaagttgtg agattcgaag ttggtgaagg tatacaaaag
     4261 agagaagaaa actttgcaga agaagttgct aagcaactta agtaataacc aaagagagaa
     4321 cacgcaggtg ttctcttttt taaaaaatag aaaaaattgg atatgaagga ttgaaatagg
     4381 aggtttctga tggataaacc aatgtacaaa agggtattgt taaaattaag tggagaagca
     4441 ttagcaggag aaagaggttt tggtataaat aatgatgtag ttaatgatat tgctattgct
     4501 ataaaaaaaa tacaagaaat aggtgttgaa gtagctgtag tagtaggtgg aggaaacttc
     4561 tggagaggca gaaccagtga aggaatggac agaactactg ctgactatat aggaatgtta
     4621 gctacagtta tgaatgcaat ggcgctacag gatgcactag aaaatataga tgttgctaca
     4681 agagttcaaa ctgctataga catgagacaa attgcagaac catatataag aagaagagca
     4741 gttagacact tagaaaagga aagagttgtt atatttggtg caggaacagg aaacccttac
     4801 tttacaacag atacaactgc tgctttacgt gctgctgaga tggaagcaga agttatattg
     4861 ttagctaaaa atgttgatgc agtatatgac aaggatccaa aagtacatgc agatgctaag
     4921 aaatttacag aattaagtta catggaagtt atacagaaag aattaaaagt aatggattct
     4981 acagctacat ctttatgtat ggataataaa attccaataa aggtatttga gcttacaaca
     5041 gagaatataa taagagcagt aaaaggcgaa aatataggaa ctactgtaaa ataatgaaga
     5101 atgaaggagg agtaaaatta tgaaactgga aatacataag caattagaag agaaaatgaa
     5161 tgggacaatt gatgctttaa agtttgaatt tggaactata agagcaggaa gagctaatgc
     5221 acaaatgcta gacaaaataa gagttgatta ttatggaact ccaactccaa taaatcaaat
     5281 aggtgctata tctgtaccag aaccaagaat attaatgata tctccttggg ataaatctgc
     5341 tatgcatgaa atagaaaaag cgatagctaa ctcagactta ggattaaatc catcaaatga
     5401 tggtgaggtt ataagacttt ctgtacctgc tttaacagaa gaaagaagaa aagaattagc
     5461 taaaaaagct agtaaagctg cagaagaatt taaagttaga ataagaaatg aaagaagaga
     5521 cgccaatgag aaaattaaaa agatggaaaa aggcggagaa ttaactgaag atgaattaaa
     5581 aaaagcacaa gatgaagttc aaaaaatgac tgataaattt ataaaagaaa tagatacatt
     5641 attatctaaa aaagaaaagg acataatgga ggtataatat tgaaaaaata ttataacctt
     5701 ttaggtcttc atgtagatga ggtaaaacaa tattttgatg agcaaaatat aaattatacc
     5761 gtaaaatcta ttgaaggtaa aaaggataaa gaaaaacttg tagttcctag agttataaaa
     5821 atatctgaga taggtgatag tgtagaactg ataatcacat acttttctga ctccttagtt
     5881 taaggagtca gaacaatatt ggaggcaaaa tatgaataac aacattatat atgatataga
     5941 cttaaataac atacctactc atattgctat tataatggat ggaaatggaa gatgggcaaa
     6001 agctagattt cttccaagaa ctgcaggaca taaggctgga gtagaaacta taagagatat
     6061 agtaaaagaa tgctcaaaat taggtgttaa gcacttaaca ttatatgcat tttcaacaga
     6121 aaactggaaa agaccgaaac ttgaagttga cacacttatg aacttattat ctacatactt
     6181 aagaaatgaa atagctgaac ttcaccaaaa caatgtaaaa gtaactgcta taggagatat
     6241 aagtgcttta ccaaaaactt gtataagaga attaaactca gctaaggaac tgacaaaaaa
     6301 caatacagga gttaacttaa atttagcatt aaactacgga agtagagcag atataaaaaa
     6361 tgctcttata gatatagtaa aaaattgtga aagtggtaaa atagatataa acaatattga
     6421 tgaagatatt ataaaaaact atttaagcac aaagtctata ccagatccag acttggtaat
     6481 aagaacaagt ggtgaacaaa gattgagtaa ctttttactt tgggaagttg cttattctga
     6541 gttttacttt actgatatac attggcctga ttttaaaaaa gaagaactac agaaggctat
     6601 atacgtatat caaagaaggg atagaagatt tggaggactt aagtaaggag attattttat
     6661 gcttacaaga attattgctt cactagctct ggttccatta tttctatttg ttgtatatgg
     6721 aggtattcct ctatatatag ctgagacagc aatagtttat attgcattgc atgagtttta
     6781 taaggcattt aaaataaaag atgtacatcc aatattcatt ataggatatt tattttctat
     6841 ttatttagct gtaaaaaata tttttaattt accattagaa tatacttatg cagtaatttt
     6901 tattttattt ttagcaagta taatttatat gttgatgggc aaaaacaatg ttatagatgt
     6961 ttcgattacg tttttaggag ttttttatat aggagtattt ttagatttta taattataac
     7021 tataaatgga tttgaaaaag gtagtatata tgtatggctt atatttgtga tatcttttat
     7081 gacagatata tttgcatatt ttagtggtta tttacttgga aaacataagc taattcctaa
     7141 agttagtcct aaaaagacta tagaaggagc tattggagga ataataggaa gtacactttg
     7201 ttgtatttta tttggatatt tatttggcat tgatttgctt caattagcga taataggaag
     7261 tataggaagt gtaatagctc aattaggaga tttatttgca tcttctataa agagatatgt
     7321 agggataaag gattatggaa agataatacc tggtcatggg ggtatattag atagatttga
     7381 tagtgttata ttagttgcac cttttgtata tagtgcgatt aaatttttta ttagataaag
     7441 attggttgga tttaaaagag caatctataa ttgctagtaa tgaaaatagc tatgtaactt
     7501 ttaaatgcag agatattctg cagttattag ttacatggct attttcattg ttcaaaaatt
     7561 tagaagacct tggggaattg tataagaata ggtattactt tataatagta tactatattt
     7621 gtagtgagta tattttttat actataacta tcattaaaaa atattttttt gtttaataat
     7681 ttcgatattt ttagctttat tatatattta taatatatat gtataaatgt aactaattta
     7741 ttatacattt ataaatttta tttttaaata taattaataa gagataagta a
//
LOCUS       DAMJYC010000149         7789 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJYC010000149 DAMJYC010000000
VERSION     DAMJYC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.155"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(224..2503)
                     /locus_tag="PC472_003672"
     CDS             complement(224..2503)
                     /locus_tag="PC472_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2728949.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2774..4228)
                     /locus_tag="PC472_003673"
     CDS             complement(2774..4228)
                     /locus_tag="PC472_003673"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2728950.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4392..5261
                     /locus_tag="PC472_003674"
     CDS             4392..5261
                     /locus_tag="PC472_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2728951.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5474..7546)
                     /locus_tag="PC472_003675"
     CDS             complement(5474..7546)
                     /locus_tag="PC472_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2728952.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2643 a         1291 c          827 g         3028 t
ORIGIN      
        1 ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa cattcaaatg
       61 taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa
      121 tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag
      181 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
      241 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      301 caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta
      361 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      421 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      481 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      541 aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata
      601 tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa
      661 tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta
      721 ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa
      781 aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaaa ccatttttct
      841 caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt tgccaacgta
      901 caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa
      961 ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta
     1021 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
     1081 ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt
     1141 caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1201 tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct ctattatttt
     1261 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1321 taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc
     1381 caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct
     1441 ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa
     1501 tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta
     1561 taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca catgctgata
     1621 atgtaagttt tataatagca tatgatttct cagatatagc agttttagga acttcagaaa
     1681 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca
     1741 tactataact taatgaacct gatttaaagt tttttatatt tgacttcata ttgttgatac
     1801 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1861 tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat
     1921 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1981 tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa tatacattaa
     2041 tattactatt ggtattttta tcatcttcta ttacatcttt taattttcct gttataacca
     2101 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     2161 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2221 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa
     2281 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2341 tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2401 tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2461 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2521 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2581 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2641 attaattaat atagatatta gtgtttacat tattgcatta tctattttac aattatttac
     2701 atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg
     2761 tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2821 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2881 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa
     2941 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     3001 gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa
     3061 ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa
     3121 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag
     3181 tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc
     3241 ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta
     3301 catagcaagc agctataact gctaaagtat tatatactgt aaacatccct ggaactggta
     3361 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3421 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3481 catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta
     3541 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat
     3601 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3661 tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccattgca cagtagtcac
     3721 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3781 aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt
     3841 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3901 tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta
     3961 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta
     4021 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     4081 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     4141 aatcatattg tacattatta atatctatat ttaattcacc cttgacattg attatatcca
     4201 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4261 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4321 gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4381 ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata
     4441 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4501 tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt
     4561 gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat tcaaatgtct
     4621 tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta
     4681 gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt
     4741 taaatatgca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4801 ccaatactga atttggaaat gaaatattga gaaatacatt atttatacaa cttatgatat
     4861 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat gttttttatg
     4921 atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa
     4981 tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     5041 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt
     5101 cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     5161 attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa
     5221 acaataaaaa tcctttagat attactcttt tagatgaata gataatttat gtaaatttta
     5281 ttttaataaa acttgaagta ttagtgtatt tatcttacta atacctcaag ttttttaatg
     5341 ttttaaaata tatcaaaata taatgatata tatttctatt acttaaaata tatattaaat
     5401 aaattgcatg ttaactgaac ctatttatat atttattaaa tctcacataa cttttagtta
     5461 cactatcata tagctatcct attttttcaa tatcatctct taatatactt actctattct
     5521 cctcttttgc tgtggttact ccttttttaa tgcttaattt ctgattttgt tctaaattcg
     5581 taaatactac aggacttatt gttgatagac catttttttc aatatattct aagtccattt
     5641 taactaattt ttctcccgct tttacttcat ctcctacatt cacaaatact tcaaatccat
     5701 ttccttctaa gtttacagtt tctatcccta aatgtataag tatttctact ccactttttt
     5761 gggattttat tattattgca tgcttagttg gaaatataac tccaatagtt ccatcaactg
     5821 gtgaataaat caatccatca ctagatttaa ttgcaaatcc atcccccatc agtttacttg
     5881 aaaatacaga atctggcact tcttctatat ctaataactc tccatttgtt aatgaatcaa
     5941 tcttttctac taaagtattt tccatatttg caacttccat attttttttc actttatttt
     6001 tatcaacatt tttagtaaca gtactttcaa cctttgtttt tccctctatt atatctttta
     6061 tatcagactt tattgaatct gattttggtc caaatatagc ttgaacattg ttgccaataa
     6121 ccataacacc tgctgctcca agtgatttca atttttcttt atctacttta tttatatcat
     6181 ttacaaccaa tctaagtctt gttatacacg catctagact ctttaagttg tctttatttc
     6241 ctaaagcaat caaaatctct gatgctaatt cattcttaga catttcaatg acttcagcgt
     6301 tagtttctga ctcatcttct cttcctggtg tctttaaatt aaattttctt atcataaatc
     6361 taaatacact atagtataat actgcaaaac caatacctac caagatagcc aagtaccatg
     6421 gagttctatt aggaagaaca ccaaataata agaaatctat taatcctcct gaaaatgtga
     6481 atccaatttt aactcctaat aattgcataa ttgcgtatga taaacctgaa aataaacaat
     6541 gtattgcaaa taatactggt gcaacaaata aaaacgcaaa ctctattggc tcggttattc
     6601 ctgttaaaaa agaggttaat gctgctgatg ccattatacc tgctacaaat tttttctttt
     6661 ctggctttgc ttcatgcacc atagcaagag ctgctgctgg aagaccaaac atcataaatg
     6721 gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac tccatcttta agttgagcaa
     6781 agaatatctt ttggtcaccc attacaagct caccagcttt atttacatat tctccaaatt
     6841 gataccaaaa tggattatgg aaaacatgat gcaaaccaaa tggtattaat agtctttcca
     6901 aagaaccttc cataaatgct gctagtgttt gatttgcttc tataacattt tttgagaaac
     6961 taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa tataattcct attgctatag
     7021 caactaatga agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct agatactgag
     7081 gtaattcaat tttataaaac tttttataaa gataagcagc cactatccct ataataatac
     7141 caccaaatac ccctgtttga attgttggta ttccaacaat atttgcatac atagaattgt
     7201 ctaccatatc ttctgtaaca cctgccataa cacccataga tgtattgaga attaaaaaac
     7261 ctactatggc tgccaatgct gcaacacctt ctccttcagt taaaccaaca gctaccccaa
     7321 ctgcaaatat aagtggtaag ttattaaata taatttgacc tgaactttcc attatactta
     7381 ttataagttg aaatgtagga ttcgccaaag ctggaaaatg agccaacaat gcgtctcctg
     7441 ctaaagcatt actcacgcca agtaatatac ctgctgcagg aagtaatgca actggtagca
     7501 ttagagattt tcctactttt tgtaaaacac caaatacttt tttcatttac ttttctctcc
     7561 ttattatttt atattttttt aaataaaaaa gacttaagtt tataaaaagt tacattagta
     7621 tacttaattc taaaattaaa cttatgttaa tataaacaaa cctatattaa cctaatttca
     7681 gaattaaaaa taaactatta taattttttt ataaacataa gtcttgcctg cattatcagt
     7741 aacaatctta atgttttaaa attattattt tatattaaat tttaaataa
//
LOCUS       DAMJYC010000150         7754 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJYC010000150 DAMJYC010000000
VERSION     DAMJYC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.156"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            316..1509
                     /gene="nifV"
                     /locus_tag="PC472_003676"
     CDS             316..1509
                     /gene="nifV"
                     /locus_tag="PC472_003676"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2728953.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1534..3459
                     /locus_tag="PC472_003677"
     CDS             1534..3459
                     /locus_tag="PC472_003677"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2728954.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3481..4476
                     /locus_tag="PC472_003678"
     CDS             3481..4476
                     /locus_tag="PC472_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2728955.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4802..5275
                     /locus_tag="PC472_003679"
     CDS             4802..5275
                     /locus_tag="PC472_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2728956.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5309..5803
                     /locus_tag="PC472_003680"
     CDS             5309..5803
                     /locus_tag="PC472_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2728957.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5900..6421
                     /locus_tag="PC472_003681"
     CDS             5900..6421
                     /locus_tag="PC472_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2728958.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6610..6921
                     /locus_tag="PC472_003682"
     CDS             6610..6921
                     /locus_tag="PC472_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2728959.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6949..7635
                     /locus_tag="PC472_003683"
     CDS             6949..7635
                     /locus_tag="PC472_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2728960.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3107 a          762 c         1500 g         2385 t
ORIGIN      
        1 taaattttag taggaatttt ataggtttat ttttaaaggt tttttgaaaa taaatctatt
       61 taaaaattaa attaaaatgc aagaaaaaca taataacttt catttttaag aagttttcac
      121 aaaaaagaaa aaatatagtg caataaatga tacaaagtaa tataatagat tacaagatat
      181 gaaaagctaa aaaaataaat tgcaaatgaa attaaaatga ataaaaaata aaaaaataat
      241 cataagttag aggtatcaat gaaaaataag acttaatgtt tttaaaatta aaagtatact
      301 aaggggggca ttaatatgtg cgttatatca aaagatagag caaaggaaat aaaaatagta
      361 gatactactt taagagatgg agagcaaaca gctggggttg tatttgcaaa tagagaaaaa
      421 ataatgattg ctgaaatgtt aagtgaccta ggtgttgacc aaattgaagt tggtatacct
      481 actatgggtg gagatgaaaa aaatgtaata aagcacatat gtagtagaaa tttaaaatca
      541 gatataatgg cttggaatag agcagtaata aaagatgttg aagaatctat ttcttgtggt
      601 gttgatgcag tagctatatc aatatcagta tctgacattc atatagaaaa taaattgaga
      661 acttctagag gctgggtttt agaaaatatg gctaaaacag ttgaatttgc aaagaaaaat
      721 ggattatatg tttcagtaaa tggagaagat gcttcaagag cagatataga cttcttaact
      781 gaatttataa atgttggaaa acaagcaggg gcagatagat ttagatactg tgatacagta
      841 ggagttatga atcctttttc tataaagaat gcaatagaaa ctctttatga aagaactaat
      901 tttgatatag aaatgcatac acataatgac tttgggatgg caactgcaaa tgcacttgca
      961 ggaatagcag caggagcaaa ttatgttggt gttacagtaa atggattagg tgaaagagca
     1021 ggaaatgcag cattagaaga agttttaatg gcacttaagt gtgtttataa atgtgattta
     1081 aataatatag atactagaaa atttagagga atatgtgagt atgtagcaca ggcatcagga
     1141 agaatacttc ctacatggaa accagtagtt ggggataata tgtttataca tgaatctggt
     1201 atacatgcag atggagcttt aaaagaccca cataactatg aaccatttga cccaagtgaa
     1261 gtaaatcttg aaagaaaaat agtaataggt aaacattcag gaagagctgc agttgtaaat
     1321 aagttgagtg aatatgaaat gtatatttct ccagaaaacg ctaccaagct attaaatgct
     1381 ataagagcta catctataag attaaaaaga agtttaatgg ataaagaaat tcttcaatta
     1441 tattgtgata tactagcaca tgagaaaggc acaacagaag aagaagcagt tagaggttca
     1501 tatatttaag atataattgt ttaggaggaa aatatgggag ataatatagt ttataaaata
     1561 attaaaaaac atatagtaga tggagaagct gttgctggaa gttcaattgg tataaaaata
     1621 gaccaaactt taacacaaga ttctactgga acaatgacat atcttcaact agaagctatg
     1681 ggaatagata aagtaaagac taaaagaagt gtggcatttg tagaccataa tatgttacaa
     1741 caaggatttg aaaatgcaga cgaccataag tatatacaaa cagtggcaga taaatatgga
     1801 gtttatttct ctaagccagg aaatggtatt tgtcatcaag tatttttaga aagattttca
     1861 acaccaggag atacactttt aggttctgat agccacacac ctactgctgg aggtgtgggc
     1921 atgatggcta ttggagctgg tggattagat gttgctttag ccatggcagg aggagcatac
     1981 tatataaaag caccaaaagt ctgtaaggtg aatctagttg gaaaattaaa taatatggta
     2041 tcatcaaaag atataatatt agaagtgtta agaaaacaaa ctgtaaaagg tggagtaggt
     2101 aaagtttatg aatatggtgg agaaggtgtc aaaagcctat ctgttccaca aagagctaca
     2161 ataacaaata tgggggctga acttggagca actacttcaa tcttcccaag tgatgaaaaa
     2221 acactagaat tttttaagag tcaaggaaga gaagacgcat gggtagagct aaaaccagat
     2281 gcagatgcag tttatgatga agagattact ataaacttgg atgaattaaa accattagct
     2341 gcaaaacctc atagcccaga caatgtagat gaagttgaaa atataggcaa gattaaaata
     2401 gaccaagtgg caataggaag ttgtacaaat tcttcttatg aagacttaat gaaagtagca
     2461 caaatcctga aaggaaataa agtacataag gatgtgagtt tggtaatagc accaggttca
     2521 agacaagtta tggagatgat tgctagaaat ggggctttgg cagatataat aagtgctgga
     2581 gctagaatac tagaaaattc ttgtggtcca tgtattggta tgggtcaatc tccaggtact
     2641 gactctgtgt ctttaagaac tttcaataga aatttctatg gtagaagtgg gactttgtca
     2701 gcacaagtat atttggtaag tccagaagtg gcagcagtaa gtgcaatcaa gggagtgctt
     2761 acagacccaa gagaatttga tattaaattt acaaatttag atgtaaatga gtttttgata
     2821 gatgatagca tgataattaa acctgctgat gtaggaagtg atgtagaagt agttagagga
     2881 cctaatataa aacctttccc tcttaataca gagctaagtc agtctatagg tggaaaagtt
     2941 atattaaaga cagaagataa tataactact gaccatataa tgccatcaaa tgctaagtta
     3001 cttcctttta gaagtaatat accgtatctt gcaaactatt gtttcaatac tgtagataca
     3061 gaatttcctc agagagcaaa agacaataat ggtggattta tagttggtgg agataattat
     3121 ggtcaaggtt caagtagaga acatgcagct ttagcaccat tatatctagg tgttaaaggg
     3181 gtaatagtta agagctttgc aagaatccac aaggctaact tgataaatag tggtataatt
     3241 ccaatggagt tttgtgatga gaaagattat gaaaatatat ctcttttaga taatctggag
     3301 ataccaaata ttttagacaa tctaggtagt ggaattttag aagttaagaa tacaactaaa
     3361 ggaacttctt ttaaggtcaa agtagaattg tcagcaaaag aagttgatgt attaaaagct
     3421 ggtggaaaat taaactacac taaaaatcaa gctaattaat atgaaaagag ggaatattga
     3481 atgtataaag tgacacttat accaggagat ggaataggac cagaagttgc gaaagctatg
     3541 aaaaaggtag tagaagctac tggagtagaa atagaatggg aagaagttaa tgcaggagag
     3601 gcagttattg aagagtatgg tactccactt cctgaatata ttatagatag tataaaaaag
     3661 aacaaaatag ctataaaagg cccaattact actcctgtag gtaaaggatt taggagtgta
     3721 aatgtaactt taagacaggc attagatttg tatgtcaatt taagacctat taaaagtttt
     3781 aaggggataa aatcaagata tgaagatgtt gatttagtgg ttgtaagaga aaatacagaa
     3841 gatttatatg ctggaataga acataaaata ggtgattatg cagctgaaag tataaagata
     3901 ataacaagaa gtgcaagtga aagaatcgtt gattttgcat gtaactatgt taaagataat
     3961 aagagaaaaa aagttacagc tatacataag gcaaatataa tgaaaatgtc tgatggattg
     4021 tttttagatg tgtttagaga agttgcatca aaacatggtg tagaatatga tgatttgatt
     4081 gtagatgcag cagctatgaa cttagtttta aatccagaaa attatgatgt aatggtaatg
     4141 ccaaatcttt atggagatat actatcagat ttaggtgctg ggcttgttgg aggtttaggt
     4201 ataattccaa gtgcaaatat tggtaaagat tgtgcgatat ttgaagctgt tcatggaagt
     4261 gctccacaaa tagcaggtca aaataaggct aatccaacag cacttattca atcatcagta
     4321 atgatgctta gatacttagg tgaatatgaa aatgcacaaa aaatagaaac agctttagaa
     4381 aaagtatttc ttgaaggaag taaattgact gtagatttgg gtggctcagc ttctactaca
     4441 gagtttgcgg atgaggtgtg taagtatatt gtatagattt tatttaggga aaaagtaaaa
     4501 tatagattga aggaaagagg ctttctcaaa atagagataa gcctttttta ttggaatgat
     4561 aaaatatatt tgattttata caaaagagag ttcctcataa acgaaaaagt taattttggg
     4621 atatccattt taattttaaa attaattgat taatatgcaa agaaagatta aataaattta
     4681 ttatttttaa ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt gaactttcaa
     4741 ctaaaaatga atatattgat tatgtgaaat tctataaaac agaaactatt tgactttagc
     4801 catagtagtt tcagtggagg tgtgtttgat taagacttta gacagtaata tattaagaga
     4861 agtaggtact ttatctagag cagtaaattc aataaatgat attaagtaca aggaattaaa
     4921 gttacaaaaa ggacaattta catttttgac aagaatctgt gaaaatcctg gtataaatct
     4981 tgtggaatta tcaaatatgt taaaggtaga caaggctaca acaaccaagg ctatacaaaa
     5041 attaataaag gcaggatatg tagataaaaa gcaggatgag tttgataaaa gaggatataa
     5101 tttaactcca actgataaat ctttggaagt ttatgagttg ataattgaag aagagaatag
     5161 aagtatagag atatgttttg ataattttac agatgaagaa aaacaagtgg taactaagtt
     5221 attagaaaaa atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga gatagtaaat
     5281 aaatacatta acttaaggag atatttttat gattagaaaa gcgaatatga atgatttaga
     5341 aagcattatg aaaattataa aatctacagt ggaagaaatg aagacttata ataatacaca
     5401 atgggatgaa aactatccat tagaaaaaga ctttgtttca gatattaaga aacaagattt
     5461 atatatttat gaagtggatg gagaagtagc agggtttata tgtcttaatt atgaagagcc
     5521 agaagaatat ttaggcttaa attggtcatc taataaaaaa gcaatggtaa tacatagaat
     5581 ggctgtaaat ccaaacttta gaaagacagg aatagcatct aagttagttg attttgctga
     5641 aaaattagct gtagaaaaca atgtatcata cttaaagagc gatacatatt caataaactc
     5701 aaaaatgaat ttattactta caaagtgtgg ttttataaaa atcggagaaa tgagtttttt
     5761 aggaaaggag aaatctttct actgttatga taaaatatta taaagagcat ttacaataca
     5821 aggggtaaat aatagatttt cagcattagt aagggcataa tataacctag tatatgctat
     5881 tatgaaaggg atgatttata tgattggatt tctaaagaaa agaagaagta ttagaaaata
     5941 caaggatgtt gaagtagaaa aagaaaaatt agataaaata ttaaaagctg ctcttttagc
     6001 tccatcatca aaggggctta gaacatggga atttatagtg gtagatgata aagaaaagct
     6061 aattaattta tctcaatgta gaactaaagg tggtggattt ttcttaaaaa atgcaccttt
     6121 agctattgta attattgcag ataaagaaaa aaatgatgtt tggatagagg atgcaagtat
     6181 agctgcttca tacatacaac ttcaagctca tgaactaggt ttaggttctt gttggataca
     6241 agtaagaaat agaatgcatg atgataatat agaagctgat aagtacatta gagaagaatt
     6301 aaaagtacct agcaagtaca gtgtagaatg tataatttct ataggatatc ctgatgaaga
     6361 aaagaaagca tatagtgact ctgatttaga ctataaaaag gttcatttta acaactttta
     6421 gtatataata tgttgtatta agacattata tttttttcta ttgttaatat atacttaaaa
     6481 ttgtaaaaat atatctgaat tttactaact tatctagggg ttatgtacaa ggattttaaa
     6541 gataaatata gaaattaatg tttataatcg atacataact tgagtaacaa aatttaagga
     6601 ggttaaagta tgggtatatt taaaacaaaa atggatgagg actggaaagt aaactacatt
     6661 aaagagttta atgaaatgag agattcttat gagagtaaac ttcaaaaaaa acagtttgag
     6721 gtagatagtt tgaaatcaga attggataga ctaagaagct ataaaaatag ccttaagcca
     6781 aaagaaaaac aaattactga tgatgatata aataatataa agagtttaag aagagatggc
     6841 ttaagctata aagaaatttc aaatcaaaca agttggagca aagcaactgt aagtagggta
     6901 ttaaatggac tttacgatta gcatatacat ggggtgattt aaattgggat gaagtatagt
     6961 tacaaaaaat gcataataga ttctatttta ttgatgttta tagttcaaat attaaggatg
     7021 attttgaatt atgtacttct aagtcaattt gaatttactc ttgaaaattt taatattata
     7081 aacttaattt cttttacatt agttggttta tcgttaattt tatttttaaa agataattct
     7141 ttgtataaca aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa taaaaacaat
     7201 atacttattg aaaagtgtaa gctgatactt tttgttgtgg ttttatcttt agcaataatt
     7261 gtaacttatt gtactaaggg atatgtactt tttaatgtaa ctatgatgac tttgtctgta
     7321 ttgatagttc caatatttga agagttattt tttagagaat atatatggaa ctatctaagt
     7381 aattttataa agtcaaagag taaaattatc tgtataacat ctatactatc tggaatttat
     7441 aatataggat acatagatgt tataagaaat tatgtaatat tgtataacaa ttcgagctac
     7501 acctttgaag ttattatttc aaaaattatg ataggtactg tttttggtat tgtacttgga
     7561 ttagttaagt ataggtttag agatgtaggg ttttgtatat tattgaggag cttatttgct
     7621 atatttatta ggtagatagt ataatcggtt tataaggttg ttttaaattt aattttaaat
     7681 caaccttatt ttatttgtgt tttataaagc acttatatat taataactaa taaatttaat
     7741 aaaaattatt tgaa
//
LOCUS       DAMJYC010000151         7722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJYC010000151 DAMJYC010000000
VERSION     DAMJYC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.157"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            953..2311
                     /locus_tag="PC472_003684"
     CDS             953..2311
                     /locus_tag="PC472_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2728961.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="PC472_003685"
     CDS             2418..3212
                     /locus_tag="PC472_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2728962.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3205..5511
                     /locus_tag="PC472_003686"
     CDS             3205..5511
                     /locus_tag="PC472_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2728963.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5650..6927
                     /locus_tag="PC472_003687"
     CDS             5650..6927
                     /locus_tag="PC472_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2728964.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..>7722
                     /gene="yqeC"
                     /locus_tag="PC472_003688"
     CDS             7110..>7722
                     /gene="yqeC"
                     /locus_tag="PC472_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2728965.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVEN"
BASE COUNT         3026 a          835 c         1442 g         2419 t
ORIGIN      
        1 cttgtatatt ttatgttgca gatacaaata tagtaaagga gagtacattg gtacaactga
       61 gatacatagc ttattaagtt tttctgtctt atgagaatta tattataaaa tattcataaa
      121 tattttgagt ttaaattaaa taattatttt atgcttagat taattgatat ttaattaatt
      181 taataacaaa aacagtgaaa gagtaaataa tattatttac agggggtaaa gcaaagtatg
      241 ttaaataaat tgtacttttg gccccgtaca tagagaaatc tatgtaggac acaactttaa
      301 aaccagtaaa tcctaaagcc ttgcaactac aataaaagat agaaagaaac ttttatgaat
      361 gtggtgaaaa ccagaaacaa tagtgaggat ggtatatggt gagagcctaa gtacttaaaa
      421 atggacattt ggtcgccaag cctagaaata ggaaggtcaa acgactatct cgagagagag
      481 tacactataa gcgattgata gtggaaacgg gttgctctta gcaggtaatg ctgaagatga
      541 agaaatagtc tacgctcata tgaaagtatg aggtcactgg atttgaagtg acatctataa
      601 agtagcgttt ataggtaaat gtttaaaact tgaaacatgt aaatacttat gaaaacttat
      661 aacttataat gattaagatt gaaagattta aattgaatta aatttactat aaaaaattaa
      721 aaattcatag ctataatact cttatatttg taaagcatgg tatataatgt atacaaaatt
      781 aaagaatacg tttatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa
      841 cttaaatata tgttggagta tagttgatat ttatgaacat cattaattta atatattaga
      901 tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc
      961 aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt
     1021 agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc
     1081 aaatgcagct ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt
     1141 tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt
     1201 aggaggagga ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc
     1261 tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag ctggtatagt
     1321 aataattata attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc
     1381 aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc
     1441 tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt
     1501 atctcttatg atattaggat taaataaata tggaaaagga ttatttaaag atgcatcaat
     1561 attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc
     1621 ttcaattcag gagtttactt tggttgcact tccaaaacca cttgcttttg ggctagatat
     1681 aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat
     1741 gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact
     1801 ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac
     1861 tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa
     1921 atttgtacta gctattggag gaattggatt atttttggca ggaatatctc ctttattagc
     1981 taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc
     2041 aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag
     2101 catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct
     2161 tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc
     2221 aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga
     2281 taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat
     2341 atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt ttaataagtt
     2401 aatgatgggg ggataagatg gttactataa aggaatattc agttccgcag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggatgaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aattactgta gtggaatact ttcaagtgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtatg
     2761 gcttttctga cttaatacca gcacttttgg ttgttggagc gaaggtgaga ttatacaata
     2821 aaggaatact agagttaagt gagtttttgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcaatag ctgtttttga ctcaataaga aaatgcacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag cacttgaagc gagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatgatt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg ggttgtgggt atttatacgt ataaagatgt accacaggtc
     3421 agatattgtg ttccaggtca aacagaacca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tagctgctga agatgagaaa
     3541 acagcactta aagccttaaa aatgataaag gtagattatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagag
     3661 ccataccctt ttggattgga tgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ttgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcattacaa tcaccacaca atgtaaagag aatacttaga
     3901 aaagtattgg gtataccgtc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagtaga
     4081 catgaaatgt tttttgatgt taaagttggt gcagataaag aaggaaattt aaaagcaata
     4141 gagctaaagt cattaaacaa tacaggtgct tatggtggga atggtgtctc tgtttcttcc
     4201 gaatctggac acaatacctt accaatatat aatgatattg atgctgttag atttaatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gagggtatgg tgctacacag
     4321 ggaatatttg caatagatag tgctattgat gaattggcac ataagattgg aatggatcca
     4381 cttgaattta gaattaaaaa tataatagat aggtatacca atggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagct gagacaatgt atagaaaagg gaaagaaatt aattaattgg
     4501 gatgataaat atccatgtaa agatatagga aacaacaaag taagagcagt aggtatggca
     4561 gtagcaaatc atggttctgg tataccgcgt gtagatatgg caacagtcac tataagaatg
     4621 gatgaagatg gcacatataa actattatct ggtcttgctg acttaggtca aggggctgat
     4681 acaattcaag ctcaaattgc tgcggaagct ttaaatacta ctgtagatag aatttctatt
     4741 tatacaggtg attcagattt atgtccctat gatacaggag catttgcttc ttgtacaaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagatat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agacagggtt
     4921 tttgttaaaa atgataatag caagtttgtt ctattagagg aattgggtag agaatctgtt
     4981 ggaggaatag gatttgctag tgatgatgta ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttga aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaagt cataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aatccaactt tagctagaat acaagtagaa ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgaag accctatatt tgataaagat ggtagattat taacagatag tttcctacag
     5281 tataaagttc ctacaaaaaa agaagttgga aatatattgg tagattttgc tgagaatttc
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagttataca tacaccagca
     5401 ccagctatag caaatgcaat atttaatgct acaggagttc gtttaagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aataataata aacaattata ataaataata
     5521 aggtgtataa aagcaagtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgtcaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaga tgtgcttctt agctaagaac
     5761 ttatataatg ttggtacata taatgtaaga cagcattatt tcaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgcaaa gaaaatgaga actataagct tctcaatagt
     5881 aatatagcgc aacaaatatt aaaagaagta gacagttcgt ttaggtcatt ttttggatta
     5941 attaaattag ccaaagaagg aaaatatgac tttagaggga taaaatcacc taaatattta
     6001 gataaagaag gattttttag cataataata ggtcaaataa gaatcaaaga agatgggatt
     6061 ttaaatgtac ctatgtctcc agcatttaaa aaactatatg ggaaagtaag tataaaagtt
     6121 ccacaaaata tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttgggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaagc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaagttag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctgtgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaaa tcagattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaaatat gactttaaag gggaaagaat aacgcgtgga
     6781 cagtacaaat ccagtgttgg aatcattata aatgcagatg taaatgcgag tcttaatata
     6841 ctaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc taactataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata gaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgtt tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttcttctta caacaacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtatg tggtagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac caatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa actacaaaaa
     7501 ccatagggat attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaag tagtattaat agtagttcta
     7621 cagtaagtat tgaaaactta aaaaatatag tattaaatcc aaatggatta tttaaaaatt
     7681 attctggaaa aagagtatta tttattaata aagttgaaaa tg
//
LOCUS       DAMJYC010000152         7593 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJYC010000152 DAMJYC010000000
VERSION     DAMJYC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.158"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(283..678)
                     /locus_tag="PC472_003689"
     CDS             complement(283..678)
                     /locus_tag="PC472_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2728966.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            819..1211
                     /locus_tag="PC472_003690"
     CDS             819..1211
                     /locus_tag="PC472_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2728967.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1311..1727)
                     /locus_tag="PC472_003691"
     CDS             complement(1311..1727)
                     /locus_tag="PC472_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2728968.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1742..2653)
                     /locus_tag="PC472_003692"
     CDS             complement(1742..2653)
                     /locus_tag="PC472_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2728969.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2835..3857)
                     /gene="splB"
                     /locus_tag="PC472_003693"
     CDS             complement(2835..3857)
                     /gene="splB"
                     /locus_tag="PC472_003693"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2728970.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(4084..5214)
                     /locus_tag="PC472_003694"
     CDS             complement(4084..5214)
                     /locus_tag="PC472_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2728971.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNSGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(5602..7539)
                     /gene="htpG"
                     /locus_tag="PC472_003695"
     CDS             complement(5602..7539)
                     /gene="htpG"
                     /locus_tag="PC472_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2728972.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKHYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLFIEL"
BASE COUNT         2484 a         1302 c          791 g         3016 t
ORIGIN      
        1 tttttatagt cataatattt ttttcagatt aaataaaaat caattatagt taaaatattt
       61 tttaaaagaa atctatccca ttataaagta aattttaatt atcttatttc tagtatgtaa
      121 ttattcaaaa tagtatatca ttattgaaat acacatctaa taactaaaaa tttttacata
      181 ttcattattt aaaaatcttt ttattgcatt taaattaaat ctatcaacat ttcagtaata
      241 acgttttctt aattttcact atggatgtcg attcaattat aattaaagct ctacatcaat
      301 ctttttacca ctcattgaac aacaactaac ttttgcctta tcaatagaga atatttcaaa
      361 cttaccatct cctacagagt acatctgacc taaacttggc attatttcaa gtgccttagc
      421 ttgtgtttct tttgatgtaa caaatactgc ttcacctaaa atacgtagag tattaaggtt
      481 ttggtctata caacaaagtt caacctttgg attaaaaata agttgcttaa acatatcctt
      541 ttgattagat gtgcaaaatg taagtttacc attgtattcc ataacaaatc ctaaaggacg
      601 cacatgtgct tgattgttct catcaactgt tgctaaataa taaactcgtg ccttgttcaa
      661 aaattctaat gctttcataa ttcttacacc ccttttaaat acttgtatta aggattatcc
      721 tttgatataa ttttaatata aaatacagaa aaaacaagta tgcagttttt tctgataaag
      781 tataaaaaag atactactat aactaaggag taattaaaat gagtataaat actgaaaaaa
      841 atataactaa aaacgttgaa gaattaacct gtccaatccg ttatgcatta gacattgtcg
      901 gtggtaagtg gaaattacct attatatgta tgttagcagt tgaaaatcca attagatata
      961 gtagcataaa aagaaaattg gatggcatta caaatactat gctagctcaa tcattaaaag
     1021 atttagagtc tactggtatt gttcatcgta aacaatataa tgaaattcct ccaaaagttg
     1081 aatatacatt aacaagtaaa ggtaaaagta ttgtgcctat tttacaacaa tttgctaatt
     1141 ggggtgctac taatatgcaa gaaaaaaaca catgtggact tagttgcaaa gaatgtagaa
     1201 agataaagta aatttaaatt tactaaatta ttcatattat agactatata ttaatttatc
     1261 tgattgaata taaaaacagg atgacttata tattcatcct gttctatatg ttaagctcta
     1321 aatttaatta gttcaagcat atcgtttaat accaataaac tatctacttg aatcattagc
     1381 catttacttc cattaaaaaa gtttacctca ttatatattt ttttgatata atcacaacaa
     1441 gtattaataa ataaatcaac ctctactcta ttgctttcac ttacaataac caaagcttta
     1501 aaataacctt cttgtggata taaagtacat agtgatttac ctcttttttt atattttaca
     1561 ttccaacctc tttgcattga acatttactg tattcaagct taggttctac attataagta
     1621 gaaaccaaag ttttatttaa cttgtcccat aatggattat taataaattc attaatttgc
     1681 tcaaatgtag gtttagtatc ttgaaaaaac gcatcttgcc aattcatatt catcatcctt
     1741 tctattgttc aataggaacc ggaacaatcc aatgttccag atatgctgct tcaggtgtag
     1801 tggggtaata catctcaatt gcaaaatcag tagttgttaa attatgtttc ttcatccatc
     1861 tttccataaa tttatctgct ttaaaaacag ctgaattaac taactcatta aagttttctg
     1921 cctcaaatcc acatactaaa tattctttag ctggcaactc aaaagtacta tatccttcta
     1981 ctgatgtacc tgactctacc tctacacctg ccatatacat acaatttcct tcttttgcat
     2041 ttcccatata aagagcagca aattcatttc cacctggaat ttgatttggt atattatgtc
     2101 tttgacttcc aagtttcatc cataattcct ctactattgc aattccagtt gtttcaccgc
     2161 ccaccaactg gcaaataggt acttctattt ctattccaac aaatgtcctt ggattactca
     2221 atgtcttacg attgacttca atcactatat catctgcaat cagtggaaca ccttcatcaa
     2281 ccatagcata atttaaaatt aattctggct taataaagtg attgagtttt actggatttg
     2341 ctctatattt ttctggagtt aatccatatg cttttttaaa ggaacgtgta aatgtctcat
     2401 gactagcaaa tccaaaattt aaagctacat caagtatacg attcttatgt tctgctaagt
     2461 attcagaagc tcttgcaaga cgacgaagct ttatatactc cattacagtc ttatttacca
     2521 atcttttaaa tagtctttga tagtaaaatg gtgataaatt tgctttattt gctaattcat
     2581 taattgatat ttcttctgat aaatttgctt ctattaaatc caatgtaatt tgaatagact
     2641 cccatgcatg catactataa ctcctcctga tatttttgtt actcttatcc tttatagtat
     2701 caaaactaga aaaaatgttc ttgatagata gtgccctttt taattttctt atatgatagt
     2761 gctaaatctc attgctttat tataaacata aaatatataa aaataaaata tagcaaatct
     2821 ttaggtaaga tttgctatat tatatatttt attaaactat ttttaaatac tccatttatt
     2881 tccttaagaa agaactcttt catatcatct aattgttctt tatcatacac gtacttacca
     2941 tatccaaatt gaccgtattt aaactttcta tcttcatttt ccattggcaa tagtgtatct
     3001 ggataaatct ctagtattct attttttgct ttagttgtat atctatgaga tataacttca
     3061 aatataattt ttttatcaaa atcctttggt aaagcttctt ttatacttat aatcagattt
     3121 ttatactctt ctttccaacc ttcatataaa aacacaggtg ctattatgaa accaatctga
     3181 taaccagccc tagcaagttt tacagctgac tctattcttt tatttgcaga ggctgtataa
     3241 tgctcatgtt catttataat tctaggagta ttgatactaa atcgtatttc tgtatgatta
     3301 ttatgttcag catccaataa gctatccaca tcactgtatt tagtaacaaa tctaaatctt
     3361 cctttttcct ctcttccaaa aaactctatt gttttcttca aagaattagt atatggctct
     3421 actggaactg ggtctgatgt agctgcccct tcaaatatgg ttacctgtgg ctttctatct
     3481 tcaatatatt tttttgtctg ttcaagtatt tcatctacat ttacaaatac ccttataaat
     3541 ggtttatcac ctaattgtgt gttcaaataa caatactcac atcttcccat acatccactg
     3601 acaagtggta gttgataatt agcagaaggt ttacatgttt gaaacttcag actttttcta
     3661 atcccaacta ccatagtctg cttcccactt ctatattgtt catatagatt ttcacctgga
     3721 atatgttctt taatcctatt tgatgatata ttaattatct ctacatcttt attatctcta
     3781 aatttattgt atattttttt acccacatca gtgtctaaag ttcctttttc gaatattatt
     3841 cttttgggat taaacattta tttcacctct acttaaagtt tttaaatcga agaattttat
     3901 aaaatattac ttaattaatc ttcccttttt taaactcctt tatttattag aaagtaaaat
     3961 aaaagcttaa gacaaattta ttatatatat aacttctata cacaattttt ttactattaa
     4021 ttacttataa ttttttatta ttaatttata atttttatat aaaactaaat atgtaaagtc
     4081 aaattataaa gacttatggt acatttcctt tatttgttcc ttattaaaat actttggatg
     4141 tctcttaata tttgcaactg atattttcat acaattatca gttaaccaat ctatatctga
     4201 tttttcaata cctaaatcac ttagctttat atctaaatct atttttttaa ggaaatttct
     4261 aattgcatct gaacaatctt ctgcacaagt tcctcctaat acctttgaaa tattaccaaa
     4321 tctttcaata tcactatccc atgaactttc tataatagta ggagtcaaag ctgcaagtcc
     4381 ttttccatgc attacatctc taagaccact tacaggatgc tccattccat gtggcaaggc
     4441 tgtacccgct gtaccaatcg tcattcctcc aatagtactt gcaagtgtta tcttttccca
     4501 tgcttctaaa tcattaacat cattatatac ctttattagg ttttctgata taagttcaat
     4561 cccataaata gcttgtacat ctgcaagagg gtttcttcca ttagaaagat atgcttccat
     4621 attatgggca agtgcatcaa atccaactga tgcaattata tgttttggca ttgtaaccat
     4681 aagttctggg tcaattattg atgcttttgc atacatacta ttcttcttta tagatttttt
     4741 atctttagtt tctgaatttg taagtactgc aaaacaattt ccttcactac ctgtacctga
     4801 tgttgttgga actaagatta taggtaaagc ttcttcacct tgtctaattc caaatatata
     4861 atcatttaaa tctcctgaat ttttataaga aaaagctata ctcttggcag catccataat
     4921 acttccacca ccaagcccta aaacacaatc acatccagtt gattttataa tttctactcc
     4981 tgaatatata gtagttataa gaggattttg ttctactcta tcaaaaactt catattctat
     5041 tttagagtct tttagtaaat ctattgtttt atcaagtaat ccagttttct ttgtactatt
     5101 tttacctgtt actatcaata cttttttgcc atatttagat acttcttttc ccacatcatt
     5161 aattctgcct ctcccaaata agatatttac tggcaaatta taattaaaat tcatgctaaa
     5221 acctcctatt tctactttag taataatttt atgtttttaa caaagtgtaa ttatgtattt
     5281 aaacataaac taaaaaacaa tcttttactt aattatatgt attatcttat tatatatcaa
     5341 gttaactata tttaactaaa tagagttttt tattagaacc tacttaaata tttaaaaatt
     5401 aaccaaagat aaaaaaataa agaatggtct gtctcaaaat agagataaat atatttttca
     5461 ttgttgaaat gattaaaata aatttaattt cacataaaaa aagcgcccca tgaactgaaa
     5521 agttcatttt gagacacttc tttatcatca ctgatattga atacttttta taaatttgct
     5581 tcaatgtatt caaattcaat cttataattc tatgaaaaga ctcataagct tattacttct
     5641 ttgtacaaat gaatctaact catctggctt taattcttta ttagaaagta aagctaaatc
     5701 tgctatctgg ttacaaataa gcgtaatctt atctttcttt tcctcatcat ctttaagaga
     5761 aaccaatttt ttgattattg gactgttctc atttattaca agagtttttt cttcctcaaa
     5821 attcattcct aaatccattc cagcaaatct tgactgcatt tctgccattc taatagattg
     5881 ttctgatact aacaccatag ctggagtgtc ttcatttttc aatccttcta cagaataatg
     5941 cttcactctt tctccaacag cttctttaaa cagaccttct atcttagcta tctcttcttt
     6001 gttttcttca ctatctttat tttcattttt atcttttaat acatcagaaa ggtctgcatc
     6061 tattctatta aacttaactc cattctcttt atattccata aatgaaataa aatgagtatc
     6121 tattgatgag tttaaaacaa cagcacttaa gtcgtattct ttaaatagtt taatgtattg
     6181 agattgctgt tccttatcac ttacataaaa taccttattt tcatgtttgt ccttacattt
     6241 ttctaaataa tcttgtaatg ttatatactc atcatcaata gttttgaata atatacattc
     6301 ttttaccttc tcatagaaac tttcatcttt taagcaacca tatttaataa atatttgtat
     6361 atcatcccaa aacttattgt attcttctct ttcattttta caaagagatt ttaacttatc
     6421 agctactttt ttaattatat gcttagaaat tttgcttacg tctctgtcat tttgtaagaa
     6481 acttcttgat acatttaatg gtaagtctgg gcagtctata acacctttta ataaaagtaa
     6541 aaattctggt ataacttctt ttatattgtc agctacaaac acttgattgt tgtataattt
     6601 tacttttcct tcagtcagtt caaactcatt ttttaattta gggaagtaaa gtataccctt
     6661 taaattgaaa ggataatcta catttaaatg tatccaaaat aaaggttcat caaatacatt
     6721 gaataccttt ctataaaact ctttatactc ctcatcagta cagtcttttg gagattttaa
     6781 ccatagagga ttagtgtcgt ttaaaggctc aattactttt gcatctacaa tctcttcttt
     6841 tccatcttcc ttttgttttt tagctttttc ttctgcttct ttagcttctc tttctaatct
     6901 ttcaacatct tctaaatata tttctactgg taagaaagaa cagtatttat ttattattcc
     6961 tcttacagta aattcatcta aaaattcttt actatcatca tcaataaata gtgttatagt
     7021 agttcctcta tcatttctag tatctgattc agatatttcg tattctgtac caccttcaga
     7081 tatccatctt acaggagttg caccttctgt atatgaaagg gtatctattt gtaccttttt
     7141 agacaccata aatgcagaat aaaatcctaa tccaaaatga cctattatgt catttgactc
     7201 ttccatctta tccttatatt tattaaagaa atcttcagca cctgaaaaag caacttgatt
     7261 tatgtatttt tttatctctt cttcagtcat accaattcca ttgtcaataa atttaagtgt
     7321 accttctcct ttatttacag atactgtaat tttataatct gatgacttat tttctgatat
     7381 ttctcctagt gatacaagtc ttttatgttt acttactgca tcacatccat tacttataag
     7441 ctctcttata aatatatctt tatcagagta taaccatttt ttaattatag gaaaaatatt
     7501 ttctgtatga attgaaatac tccctttttc aaattccatt ttttatccct cctcatttta
     7561 taattaatat tttaaatttt ttaaaatggt atg
//
LOCUS       DAMJYC010000153         7543 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJYC010000153 DAMJYC010000000
VERSION     DAMJYC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.159"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(476..865)
                     /locus_tag="PC472_003696"
     CDS             complement(476..865)
                     /locus_tag="PC472_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2728973.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(2625..4031)
                     /locus_tag="PC472_003697"
     CDS             complement(2625..4031)
                     /locus_tag="PC472_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2728974.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4076..4783)
                     /locus_tag="PC472_003698"
     CDS             complement(4076..4783)
                     /locus_tag="PC472_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2728975.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     KVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            4954..5868
                     /locus_tag="PC472_003699"
     CDS             4954..5868
                     /locus_tag="PC472_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2728976.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            5868..6629
                     /locus_tag="PC472_003700"
     CDS             5868..6629
                     /locus_tag="PC472_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728977.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            6641..7378
                     /locus_tag="PC472_003701"
     CDS             6641..7378
                     /locus_tag="PC472_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2728978.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2575 a         1215 c          808 g         2945 t
ORIGIN      
        1 acttttgttt ctacctcttc agtatcttat atcaatcttt tattttttca atatcttctg
       61 ttaacacttt tatctctatc tctttaatat cttatattaa tcctttattt ttattttttc
      121 aatatcatca ttaattccta tttattttat catcttaaat acgaaaacac tgtgtcatat
      181 ttgcaaaata tatattatca tctatattaa agcttcacaa tttatattta aaaattctct
      241 ttaattacac tacctctatt taaaaagcta ccttaattat acttataagt aaaggtagct
      301 ttttaaattt aattaaatag aatatccact attaatttat atttaattta tttagattca
      361 cattatagta tcataatcca cttataatca tttgtcatat tacttaagta gactaatata
      421 ttacataaat acacttacat acactttata ttgatgctat aagaaatacg tttgcttata
      481 cgtctttctc ctcttctttt agatttttta tccactcttc aataagtaaa atatcatctt
      541 tgctcaaatt aatattttca tgtaatgaca acaataaact ttttatagaa gaatcatgaa
      601 tatttctaaa aaaatctctt gtctcaactc ctatgtattc cttttcttta attaaaactt
      661 cataatgcgt atatttatct atcttttgag aatttagaaa tctttttact actaatctag
      721 ataaaagagt caaagttgtc gtttgtttcc attgatattt ttgctccatt cctaaaacaa
      781 tatctctaga tgttactttg gaatctgatt tccatataaa tttcattatc tttaattctg
      841 attgtggcaa cttttttata gtcatgataa aaccttgcta ctcagaatat agacaagcaa
      901 tatccataat aaaatttttc gattaaatac atcttatacg gatttatata agctcagact
      961 tataagatat ttttcaatct taatcctaaa tttagctatt tgtacacggt aaatatatat
     1021 aaataatgtc aaaattctct tgtattatat tgcttaattt tatgaaatat tttgtactca
     1081 gctatctatt ttggatactt gtccagtagc tctcctttct tttaaactct ttgattttaa
     1141 atatttgtat ttcagtaaat cctacttcct aaatttttta tttactacaa cttattactg
     1201 aacttcctac ttcctaaact ttttatttac tacaacttat tactgaactt ctcacttact
     1261 aaatttctta cttactataa attataacta aacttctcac ttactaaatt tcttacttac
     1321 tataaattac tactaaactt cctactttct aaatttctta cttgctacaa attattacta
     1381 aacttcctac tccctaaatt tcttacttgc tataaattat tactaaactt cctactttct
     1441 aaatttctta cttactataa attattacta aacttcctac tccctaattt tcttacttac
     1501 tataaattat tactaaattt cctactttct aaatttctta cttactacaa attattacta
     1561 aacttcctac tccctaaatt tcttacttgc tataaattat tactaaactt cctactccct
     1621 aagtttctta cttgctacaa attattacta aacttcctac ttcctaaatt tcttacttgc
     1681 tataaattat tactactaaa cttcccactt actaaatttc ttacttacta ctacttataa
     1741 ctaaacttcc cacttcctaa atttcttact tactaccact tatcactaaa cttcccactt
     1801 actaaatttc ttacttacta ctacttataa ctaaacttcc cacttcctaa atttcttact
     1861 tactaccact tatcactaaa cttcctactt tctaaatttc ttgcttctta agaataatgt
     1921 taattttaat tttaagatta tagtttttac cttgtttaat tgaaacattc tatataatta
     1981 tatccttcta ttttagagtt tttttattta tatattacaa agtttaaatt ttttcattaa
     2041 ttatattata aattttccat atatttcaac ataaatacat atatatatat gttatttcta
     2101 aaaatactac acaaaaactc tactttagta gaacttaatg gcgcaaaata cttcaattta
     2161 aaacatttat cgtactttaa ataaataact ataataatat acttacaata gttacataga
     2221 agctattata actacacaga acagcgtata attaaaactt ttttaggatg tcattatatt
     2281 ttttcttaaa attaaaaaac actataccat aattctattt tagtagaatt tttttgtcaa
     2341 taataaaatt aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata
     2401 accttacaat aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag
     2461 ccataagcgc ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat
     2521 ttatccttat tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa
     2581 atattttcat tcttcattct tatctgttat tattttataa aatatcactt aattagaggt
     2641 aattttagta ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta
     2701 tgtgcattaa taatgccaga tactatcaaa atacctagac catgattttg acctttggca
     2761 tatgcctcac ttagtccatt ttggctcttt aataaactat taattctttc ttgactcata
     2821 ccttttccat catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc
     2881 cttatattac atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc
     2941 ctttttaata attttttatc tgcatctata ataatatctt catttagtat atcaagttca
     3001 acgtaaaagt cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac
     3061 gcagaaactc taatcttatc acaattcaat gcttgcatat tatattcaag tttagacact
     3121 aaatttaggt catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact
     3181 tgctcttgtt ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt
     3241 atcattgaaa gtggagttct tatatcatga gatattcctg caatccagtt agacctagct
     3301 tcctctttct tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata
     3361 gattttgaaa tatcacttag tattcctttt tcttgtaaaa gtactggctc tccttttgac
     3421 ataagcttta tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat
     3481 agagctatta aaaaagtaac tattgcatta accaaaaaca tacctattat taacatagga
     3541 aattgttcta tttccttaac cgtataagta aggttaaact tagtgtactt gtcttttgga
     3601 taacctgtta ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa
     3661 aagaaccttg aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt
     3721 aaatcaaatc cccatattct atctccattg ttatcaatta acattgccca aactttattt
     3781 ttatgtagta tttccttact agaatcatct aaaataaact ttccatcttt taaaaccaaa
     3841 ttatcttcaa tcataatcaa gtttgatttt atttctctgc tatcacttgt atctaaatta
     3901 tcagagtatt tagatacaaa taacatatat gaaaacagaa gaaaacctgc tatatttaat
     3961 acaataaaaa gtaatgaaat caagatactt gaacctataa atcgcctcaa tatgtacttt
     4021 acactatcca aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat
     4081 taatcatcaa cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag
     4141 ttttttctat cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat
     4201 acatatcatc tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat
     4261 tttcatacaa ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgccctt
     4321 ctttctcaac acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg
     4381 gcaactcctc ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag
     4441 taagttcctt tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa
     4501 tcaatctatc ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct
     4561 ctctaatttt tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg
     4621 cacaatctgg ctttacattt ctaaatacct taacagcttc ctcataactt gatgctaaaa
     4681 atactctact aaatccctct tttctcagga tttcatctat catttttaat aattccttgt
     4741 ggtcatcaac cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt
     4801 agtataccat atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag
     4861 gtaaatttca gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc
     4921 aataagcaca gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga
     4981 ctttcaaagg aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa
     5041 ggagctgtat atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg
     5101 cttcttggac ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat
     5161 gaaaaaaata gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac
     5221 tatggtcatc ttacagggct tgaaaacatg accataatgc aaagactctt agatttacct
     5281 aaaaaaaatg taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa
     5341 gttagtcaat attctttagg tatgaagcaa cgtcttggta ttgctatggc aataatgaag
     5401 tttcccaaat tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag
     5461 gaaattagag aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct
     5521 agccatcttt taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc
     5581 gaactggttt ttcaagatag cataaaagaa cttcataata gaggtcaaag tcagattgct
     5641 atcaaaacta agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca
     5701 cttatacaag atgggtactt gactattgaa aatttaaaag actcagatgt ggctaaaata
     5761 aatggtatgc ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc
     5821 ttggaagata tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg
     5881 gtctagaatt ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgcag
     5941 ctgttgaaat aatgtggtgt atcgtaaatt caaatagagc tattacacgt aatccagata
     6001 tgttaggtgg atttgaatat tcctatatgc ttatgagttt tgcaagttta aatggtctat
     6061 tctttcctat cttaatatca ataatcacat cacgtataag cgatatagaa cataaaggag
     6121 atacatggaa gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat
     6181 ttttatgttc tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt
     6241 tgtttggaca ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca
     6301 cattaggaac aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg
     6361 taattccaaa tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga
     6421 catcaggttt gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt
     6481 tatcaccttt aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata
     6541 tgaatttttc tatcacgatt ttagttttct taattggaat gctaatttat tatattggaa
     6601 gaaatcattt attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtctt
     6661 gggagaaatt ctaaagttaa aaaattcttt tatatggtat gtattattag ctttgccact
     6721 tataagtgtg ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga
     6781 atggtacagc ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt
     6841 aattgcaatc ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa
     6901 tgtaatgaca ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc
     6961 aatacttaca gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat
     7021 gtttggtttt tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat
     7081 tatttctatt tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc
     7141 aactcctatt ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa
     7201 acctgctgtt ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa
     7261 gcaatctgct ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac
     7321 tatagtattt tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg
     7381 gtaaatgcta gatatttttg ggtttgaaat ttatattctt tttagttgaa atctatatcc
     7441 attttattta aaaatatata tcttttattt gaattttata tgttttatct actcgctata
     7501 taatagatat actcctaaaa tttaaaaata tataataata tta
//
LOCUS       DAMJYC010000154         7414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJYC010000154 DAMJYC010000000
VERSION     DAMJYC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.160"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(22..684)
                     /locus_tag="PC472_003702"
                     /pseudo
     CDS             complement(22..684)
                     /locus_tag="PC472_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(844..1380)
                     /locus_tag="PC472_003703"
     CDS             complement(844..1380)
                     /locus_tag="PC472_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2728979.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSIRAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="PC472_003704"
     CDS             complement(1453..1866)
                     /locus_tag="PC472_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2728980.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2332..2886
                     /locus_tag="PC472_003705"
     CDS             2332..2886
                     /locus_tag="PC472_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2728981.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISGIDDTISDMQGSISSMNGTISDMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3494..3673)
                     /locus_tag="PC472_003706"
     CDS             complement(3494..3673)
                     /locus_tag="PC472_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2728982.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3899..4432)
                     /locus_tag="PC472_003707"
     CDS             complement(3899..4432)
                     /locus_tag="PC472_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2728983.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4749..5099
                     /locus_tag="PC472_003708"
                     /pseudo
     CDS             4749..5099
                     /locus_tag="PC472_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6139..6711)
                     /locus_tag="PC472_003709"
     CDS             complement(6139..6711)
                     /locus_tag="PC472_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2728984.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6773..7036)
                     /locus_tag="PC472_003710"
     CDS             complement(6773..7036)
                     /locus_tag="PC472_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2728985.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7036..7257)
                     /locus_tag="PC472_003711"
     CDS             complement(7036..7257)
                     /locus_tag="PC472_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2728986.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2595 a         1266 c          877 g         2676 t
ORIGIN      
        1 agtgtgcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacccgcc agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccatgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaaccttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactatt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttactta ctaaatataa gaaaataatt gctcaatttc tcaataaatg tgaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttaattcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgctaatt tttgtttatg
      961 actatcatag agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacactat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgccctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaatg gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct atcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattccctat catgaataga
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccattcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaagt ttaaaactaa gaataaaaag agataaaaag gaagctacta atcaaaatat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgatattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag atatatttaa
     2221 ccatgttttg aatagcagat aattatatga ttaaaaatta aatatgacat agcgtttgtg
     2281 gaatataaca cctaaattat gttataataa taaaacagga gtgtgatagt gatggatgaa
     2341 aaaatattag agttattaca ggaaatgcaa ggtaatatta gtaatatgca aggtagtatc
     2401 agtggtatcg aaggcactat tagtgatatg caaagcaata ttaatggtat cgaagacact
     2461 attagtggca tgcaaggcac tattagcgat atgcaaggca gtatcagtgg tatagatgac
     2521 actattagcg atatgcaagg cagtatcagt agtatgaatg gcactattag cgatatgcaa
     2581 gacagtatta ctgatatgca agttgaaatt aaagaagtca atagtaggtt agacaatatg
     2641 gaacctgata ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgatact
     2701 attcagagtg atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat
     2761 acagtgatta aacatacctc ttcattagta aaagatacat cagatattaa aaatgattta
     2821 aataatatag agatagtcac atctaagaat tcgaatgata ttgctaaatt aaaatcagtt
     2881 aaataatata ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc
     2941 aaattttata cagaaatact gattatccga caacacactt tacttataaa taatttatta
     3001 aaaaaatact cattttatcc taaaattaac acgactaaat gagtcttgat ataataataa
     3061 aaaataaggg agattgaaca ctaaaaagtt gtgtatctcc cttatattct ataatatagt
     3121 tatataattt ttcagcctaa aataaaagtc catctttgat taaaacacat ccccttatca
     3181 cttataaaag gataaaatct tgttttcatt tttccattta tatcttcttg cttaagagta
     3241 ataatttttt cttttgatac tttaagtgtt tggttaaatt taaccaatcg actaagtttt
     3301 gttcaacatc tatttttcat attttccaat acttaatcta ttttttataa ataattcttt
     3361 catttctaac tcacttacat tttttacata aaacgaagca tataaatttc aaccttatct
     3421 tgtatggttc ttaatactta atgagtagct taagtatcaa acattataaa ttttcccctt
     3481 acatttaaca ctcttaccat ttccaaaata tacaaaatat attttgaaca tttgtacaat
     3541 gatgaaatac aatacatgaa aatacaatat cataactgtt gtctaaaaat agaagttctt
     3601 caacataacc tttcacaaaa aacatattat ctaaatattt tttgtctgct ccttgttttc
     3661 ctgtttgtag catataaatt actgcatcta aacatataac tactcacatg aaaaaagaaa
     3721 ataaacttcc aaaatacaag ctctaaaaac tacctctaaa gttgtataat gtttactagg
     3781 aataaggttg tataacatat agtttaaata atcatttttt aaaattgcaa gttattgctt
     3841 tcaaaattag atgtttgtat atcaaataaa tgattaagaa tttctaaatt taatggcttt
     3901 ataatgcgct ccctttcttt gggataaaat aatgttgcac agatactttt tctaaagata
     3961 acaattttat ttttctatca attccaccag catacccagt caaactccca tttgttccta
     4021 caacacgatg acatggaata ataatagaga ttccattatg ccctactgca ccaccgattg
     4081 cttgtgctga cattttagat attccttttt gttgagcaac tttatatgca atttctccat
     4141 aagtgattgt tttaccatat ggtatttgtt gtaaaatttt ccacactgta gtctgaaaag
     4201 gagtacctac catatggagt gatggtgtaa aattgggttc ttttcccgaa aaataaatat
     4261 ctaaccattt ctttgtctgc tcgaaaattg gcaattcttt ttcttgatgt tcttggtcaa
     4321 gattataagc ataatacttt tgattctcaa accaaagacc agttaaccca atattatctg
     4381 atgctaaaag aatatttcca attggactat ggtaatagct aatatattgc atacaattcc
     4441 tccttatatt ctgaaaactt cactcaaatc attagacact tcttttctat ctaccattat
     4501 gtaaaaacat actaatataa tttccttcta actacacttt cgttataata tctatcctca
     4561 tatcatatta gttttatatt tacctgcatc tgttcatcct tttttccctt tccctttgat
     4621 gaatataaaa ttcatacatt taagcttcct actgtattaa cttttataga agtgtatatt
     4681 ataattttat cactaaatag gccaaaaaaa tatcgatata gcatcaagta atatcataat
     4741 actttcaaat gggaaaagta agaataggct gtaaaaaaga aaagtatttt tatgttagaa
     4801 atccagtaaa atcattagta gatagaataa cagaaaacga acaatttgct atttatcatt
     4861 ttacacataa atataattga cgcaataaag tgttttctat tctacaacga ttaataaggt
     4921 tagaaaaaag taaaaatgaa ctgtatatgt atgaagcatt agtttctgtt tctttccttt
     4981 ggcttattat gataaaaaat attgttttgc taccaataaa acacaaaaat ataattaata
     5041 tccatatgca aaaaatttta cgttatattg aaaaatacta ttgttttaca ttatattgaa
     5101 gggtactatt gtgaaaactt aattttggag tactatcaag cagtggtaat atcaataaat
     5161 cagagtgctc cagatgattt gaactaagtt taaatactat accttataaa tattttactg
     5221 agttttgttt attaaaagca actcaatttc taaaaggcac aaatgagcta ataagtgaca
     5281 ttgctataaa agttgttttt catcaaataa tctattttag taaatgtttt aaagaaaaaa
     5341 cgggatattc tccaaaagtg tacagaaata taaaaaaaga gatatgattt tttatttctt
     5401 ttgattatcc aaagagtaaa taaaaaatat tgtaaaaaac ttactaacaa cggcaatgct
     5461 ttgaactatg gtaatacttt gaacttttga ttggactttt tttgctaaaa taatatattt
     5521 aaatagcaaa gtaatctgtt taatcaataa aaaataagag ataaaagaaa tcaactaaaa
     5581 cttttacctc ttattttgat agtttatcag agctaatatt tacactcttt ttatttttga
     5641 tacttcatta aaaatgatgc ttactctcca ttcactttta attcttcatt aaaaatgatg
     5701 cttattctcc attcactttt aattcttcat taaaaatgat gcttactctc cattcgcttt
     5761 taattcttca ttaaaaatga tgcttattct ccattcgctt ttaattcttc attagaacta
     5821 tgcttactct ccattcactt ttaattcttc attaaaaatg atgcttactc tctgttcact
     5881 tttaattctt cattaaaaat gatgcttatt ctccattcac ttttaattct tcattaaaaa
     5941 tgatgcttat tctccattca cttttgattc ttcattaaaa atgatgctta ttctccattc
     6001 acttttaatt cttcattaaa aatgatgctt attctccatt cacttttgat tcttcattaa
     6061 aaatgatgct tattctctgt tcacttttaa ttctttatta aaactgatgc ttattctcca
     6121 ttcactttca attcttcatt aaaactgatg cttgcactct gtaaactcat aaacatccat
     6181 taatgcttca ccaaatcttt gatagtgaac tacttccctc tcacccaaaa atctcaatat
     6241 atctttcaaa tcagggtcat cagttaaatt tattaattga tagtacgtag ccaatgcttt
     6301 ttgttcagca gccatatcct catgtaaatc agttaccgaa tcgccaaaga cattaatata
     6361 acttgtagta aagtttactc cattagaatc tactggctga agaccatgtc caaaattagc
     6421 ataattacta ccaagtccag cagccttaag ttcttctgga gtacaattgc ctattaattg
     6481 atataacact gaagaaatta tctcaacatg agccatttcc tctgtaccta tatcagttaa
     6541 aagtgcacga gattttccag ttggcatagt atatctttgt tgaagatatc tagttgcagc
     6601 tgccaactca ccattaggtc ctcccaactg agtcatgata tattttgcca ttctagggtc
     6661 tgcttgtctt atgttaactg gatgttccag tgttttttga taaatccaca tattatctta
     6721 ctcctcctta atatatattt aataataaat ctaattttaa agtttaataa ttttatttat
     6781 aaaatgattt gtcccatggc caaggttcag atatccatga ccaaggatat tcacttgatt
     6841 catatccaaa attagttaat gctccatatt tagattcata atcacacaca gcttgtgtaa
     6901 atttatcgca gtatctgttg tatgaatcaa gagctttctt atcccttgga ttgttatcta
     6961 aatataaatt taattctata caggcaaatt gatattctga aattctctct aaaagttctc
     7021 ttctatttaa tgacattagt acatgttacc ccctttttta gctctaactc tatttttagg
     7081 atttgcatat agccaatcat tatagttatt tgactctatt aaatctaatt caggaaataa
     7141 agtacctctt tttaatgctg tttttggatt gtatgtgtca tcataaattt gacgattgat
     7201 gtatggtcta gcaaattcac aacaatagtc ttgtttatcc tcttttaaat atcccattaa
     7261 ttatcctcct taatttttag aattttaaaa tagcattatt tttaaaaatt gtatactggt
     7321 tctatattat aactggtagt tttaataggt ctttaataag attaaatgat taaataagta
     7381 tctttttttc ttattttacc tttaaaattt ttct
//
LOCUS       DAMJYC010000155         6977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJYC010000155 DAMJYC010000000
VERSION     DAMJYC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.162"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..410)
                     /locus_tag="PC472_003712"
     CDS             complement(<1..410)
                     /locus_tag="PC472_003712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728987.1"
                     /translation="MTRMERRLRKIKNLKNKTAMIGVTASLALSTISVVTPNIVVHAE
                     SLSKGAMITNLEDNTYNKIEEITKLFSENNKDKAPVYKEKILKIKSEYLEKMNGIEDP
                     SGIRNYYTEAIKKINEIFREAKKEEVKKITESKKA"
     gene            complement(469..1422)
                     /locus_tag="PC472_003713"
     CDS             complement(469..1422)
                     /locus_tag="PC472_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2728988.1"
                     /translation="MKETLNVRDNSPENKFFMKGFYFDTVVEISICCCQSKGDDILNK
                     SFDLCKYYEELLSKTKKGSDIYNVNNSGVDGVDVHDETLNIIKKSLYYSEISNGNFDI
                     SIQPLLRLWNFKNEEKIIPSQKDIETNLELVNYKNIDVNKNNVRFKISGMSIDLGGIA
                     KGYIANKIKEYLISEGVRSGIINLGGNVLCIGKKDNGEKFKIGIQRPFSERGEVLGIL
                     EVCDKSIVSSGIYERFFEKEDKIYHHIINPKNGRPFESDLVQVTIVSDDSMDGDALST
                     IALSLGEKEGIKLINSIDRVEGLFINKNGTIRVTENFESIL"
     gene            complement(2030..3097)
                     /locus_tag="PC472_003714"
     CDS             complement(2030..3097)
                     /locus_tag="PC472_003714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728989.1"
                     /translation="MNKKVKILIASAALSCGVSNFIVPQRVEAKEISTMARESRLESK
                     KSNHEVNNNKSTEKVNEARENAKSKLKAKFEEANKELKKLGDKALGKSFAEINAIYEV
                     GNIEINKAQDTNKVNEALNSATESMDKTLNEALNSAGELERILNELKPNETEKPKHEQ
                     NQMNSTLKEKVKNMFPKLEKIASANYGRYYIQGPISVLKGIVDKNFAGYSDETIKTII
                     DDIQYYFGHSVGAEVGENGVDGFIAAIDKLYSKFENKSKHEQNQMNSTLKEKVKNMFP
                     KLEKIASANYGRYYIHGPISVLKGIVDKNFAGYSDENIKTIIDDIQYYFGHSVGAEVG
                     EDGVDGFIAAIDKLYAEFENR"
     gene            complement(3386..3904)
                     /locus_tag="PC472_003715"
     CDS             complement(3386..3904)
                     /locus_tag="PC472_003715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728990.1"
                     /translation="MKVVRNLIIVLFLLLLTGCSSTPATVENHKNIVDPGNDTNKIEN
                     KKDSIASNDGTPIEIKEKMFIAQLDDIFNNSDDYIGRTIKIEGLYNSLEADGKTLNYV
                     YRHSPGCCGNDGIVCIEFVGNDKMTMLNPDDWIEVIGKIEAYKDKTFNSVRIRLETLN
                     VKTQRGAEFVSQ"
     gene            complement(3901..4731)
                     /locus_tag="PC472_003716"
     CDS             complement(3901..4731)
                     /locus_tag="PC472_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDF2728991.1"
                     /translation="MNLVSEMMSYPFIVRAVIVGILVSLCSSLLGVSLVLKKYSMIGE
                     GLSHVSFGAIAIAVALGVSPLKLSIPIVIIVAFLLLRIKESSKIKGDSAIALISSSSL
                     AIGVISIAITTGMNTDVCNYMFGSILAMSKNDVYLSVVLSIVVIFLFTIYYNKIFAIT
                     FDENFTKSTGINVNFYNMMIAILTAITIVLGMRMMGAMLISSLIIFPSIISMKIFKSF
                     KSVVISSGLVSILSFCIGMTISYKFSTPTGASIVVVNIIIFILSIIGEKIKSNNRSYI
                     "
     gene            complement(4727..5437)
                     /locus_tag="PC472_003717"
     CDS             complement(4727..5437)
                     /locus_tag="PC472_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDF2728992.1"
                     /translation="MNLVEFNNTSFSYENKLVAKDISFTVSKGDYLCIIGENGSGKST
                     LIKGILGLVKPCKGSIFISEELKDSTIGYIPQQSDIQRNFPATVYEVVLSGCIKRNSV
                     FPFYRKSDKVLAHENMEKMKISDLKNKCYSDLSGGQQQRVLIARALCATDKLIVMDEP
                     ITGLDQKTTLNLYDMLHNLNKNYGLSIIMVSHDIKTSLNYANKVVHMKNRMLFYGDVK
                     DYKQSDCGKKFLGGEIND"
     gene            complement(5440..6468)
                     /locus_tag="PC472_003718"
     CDS             complement(5440..6468)
                     /locus_tag="PC472_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDF2728993.1"
                     /translation="MRKVTVIGMSIILIILSVLTGCTRTESKENDKNNISSRKDKLKI
                     VTTLFPQYDFAKQIAGDRADVKMILSPGQDSHSFEPTPEDIKKIQNADIFIYNGGEAE
                     EWIEKIIDSVDTKKVNAIKMMDYVKAVKEEEVEGMEPEVEHDHDHEHDKEKSHKHDEN
                     DNHNHDEEYDEHIWTSPKNAKNMVEAIKNTLVKVDNTNEKYYNANYENYTKKLDELDK
                     SLTNTVAKAKRKMIVLGDKFPFRYLAKDYGLTYRAAFNGCSTQTEPSIDTVTYLIKTV
                     RENKIPVIYHIEMSNKKIAKTISDETGAKVLLLHSAHNVTKEDFDKGITYIDIMNNNI
                     GNLKEGLQ"
     gene            complement(6475..6897)
                     /locus_tag="PC472_003719"
     CDS             complement(6475..6897)
                     /locus_tag="PC472_003719"
                     /inference="COORDINATES: protein motif:HMM:NF013629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2728994.1"
                     /translation="MNNKTVYKTKQKEIILDYLKSNEDSHVTIDEVFNYLSEQGNKVG
                     RTTVYRHMEKLADDGILRKYYIEEGIGSCYQYIGEREDCHQHFHLKCTECKELIHLKC
                     SYLEDLNNHILKDHNFKINNTKTVFYGICENCRDKLER"
BASE COUNT         2160 a         1221 c          669 g         2927 t
ORIGIN      
        1 gcttttttac tttcagtaat ttttttgact tcctcttttt ttgcttctct aaaaatttca
       61 ttgatttttt ttattgcctc tgtgtaataa tttcttattc cagatggatc ttcaattcca
      121 ttcatttttt caagatactc agattttatt tttaaaattt tctctttata tacaggtgct
      181 ttatctttgt tattttcact aaataattta gttatttctt ctattttatt gtaagtattg
      241 tcttctaagt ttgttatcat tgctcctttt gaaagacttt ctgcatgtac aactatatta
      301 ggtgtcacaa cacttattgt agataatgct aatgaggctg ttacacctat cattgctgtt
      361 ttatttttta aattttttat ttttcttaat cttctttcca ttcttgtcat taaaaatcct
      421 cctttttagc ttgaagtttc ccttagaatt ttgctatgat ttatttaatt acaatatgct
      481 ttcaaagttt tcagttaccc ttatagttcc attcttattt ataaatagtc cttccaccct
      541 atctatagaa tttataagtt ttataccttc tttttcacca agcgataatg ctattgtact
      601 taacgcatca ccatccatag aatcatcaga tactatagtg acttgaacta aatcgctttc
      661 aaagggtctt ccattcttag gatttattat atggtgatat atcttatctt ctttttcaaa
      721 aaatctttca tatattcccg aagaaacaat tgatttatca catacttcta aaattcctaa
      781 tacctcaccc ctctctgaaa aaggcctctg aattccaatt ttaaattttt ctccgttatc
      841 ttttttacct atacataata cattcccccc taggtttatt attccgctac gtactccttc
      901 tgaaattaaa tactctttta ttttatttgc tatatatcct ttagcaattc cacctaaatc
      961 tattgacata ccacttattt taaatctgac attattttta ttaacatcta tatttttata
     1021 atttactagc tctaaatttg tttcaatatc tttctgacta ggaataatct tttcttcatt
     1081 tttaaaattc caaagcctta aaagtggctg gatactaata tcaaagtttc cattagaaat
     1141 ttcgctgtaa tataaactct tttttattat gttaagtgtt tcatcatgaa catcaacccc
     1201 atctacacca ctgttattta cattgtagat atcactacct tttttagttt tactaagaag
     1261 ttcctcataa tatttgcata aatcaaaaga tttgtttaat atatcatcac ctttactctg
     1321 acaacaacat atgctaattt ccacaactgt atcaaaataa aaacctttca taaaaaactt
     1381 attttcaggt gagttatccc ttacatttaa tgtctctttc atttaacttt tccgatcctc
     1441 ctaactctat ttttaaacga agttgattat tattatcatt taaaaaataa aaaatttaat
     1501 gccattatct aagatagtat cccaataaac tctataactt attaataata ctaccataac
     1561 tcaattcatt atttttatca tttttttctt aaatatataa aaaaaaagaa agatccttac
     1621 ttctttatat gttatattct cattaaacta aattttcttt cataagatta catagtctgt
     1681 ccacactact ttttagtata ataattaatt gtatcatttc agtagtttgc acagactatt
     1741 tataagttat ctagttccac ccttcattta ttttatattt taaagactaa tataaattac
     1801 catctttata attcaaaaac tatatctatg ttttatttgg taataatact aatcaaaata
     1861 tcaatgtgaa gtatcttagt ataaaccata taccataact atgaatataa atactataat
     1921 ggctataaat atataggcca aaggcataac catactcctc tcaattccat aatctgaata
     1981 tagatttaaa ttcaggttaa agcttaataa gttctatata agtaaaaaat tatctatttt
     2041 caaattctgc atataattta tcaattgccg ctatgaatcc atctactcca tcttctccaa
     2101 cttctgctcc aacagaatgt ccaaagtagt actgtatatc atctattatt gtttttatgt
     2161 tttcatccga atatccagcg aagtttttat caacaattcc ttttagtaca ctaatcggtc
     2221 catgtatgta atatcttcca taattagctg atgcaatctt ttctaacttt ggaaacatat
     2281 tttttacttt ttcctttagt gtagaattca tttgattttg ctcgtgcttt gatttatttt
     2341 caaattttga atataattta tcaattgctg ctatgaatcc atctacccca ttttctccaa
     2401 cttctgctcc aacagaatgt ccaaagtagt actgtatatc gtctattatt gtttttatag
     2461 tttcatctga atatccagcg aaatttttat caacaattcc ttttaataca ctaatgggtc
     2521 cctggatata atatcttcca taattagctg atgcaatctt ttctaacttt gggaacatat
     2581 tttttacttt ttcctttagt gtagaattca tttggttttg ttcatgtttt ggtttttcag
     2641 tctcatttgg ctttaattca tttaatattc tctctaattc acctgctgag tttagcgctt
     2701 catttaatgt tttatccatt gactcagtcg cagaatttaa tgcttcattt actttattag
     2761 tatcttgtgc tttatttatt tctatattgc ctacttcata gattgcatta atttcagcaa
     2821 atgattttcc aagtgcttta tcacctaatt tttttaattc tttatttgct tcttcaaatt
     2881 ttgctttaag cttacttttt gcattttctc tagcctcatt tactttttca gtagatttgt
     2941 tattattaac ttcatggtta ctttttttac tttctaacct agattctctt gccattgtac
     3001 taatttcctt tgcctcaaca cgttgaggaa caataaagtt gctaactccg caacttaatg
     3061 cagctgatgc tattaatatc tttacttttt tattcattct tttcatcctt tcttttcttt
     3121 acatttctaa attaattata attttgtact tttaaaatac tataatttaa catctattta
     3181 acctcaacat tttctaattt ttaaaaaaaa tattaaccaa gaaattttag aaaagttgat
     3241 atgataagaa agaaaaattt aactactttt ttacttttaa gtcctattaa catccctttt
     3301 tataaaaact aaaaagcaca tttataattt ataaatgtgc tttcatagat ttaataatac
     3361 taacctttct ttatctttat gaaatttact ggcttacaaa ttctgcgcct ctttgtgttt
     3421 taacatttaa agtttctaat cgtattctaa cactattaaa tgttttgtct ttatatgcct
     3481 ctattttacc aattacttct atccaatcat ctggatttag catcgtcatt ttatcatttc
     3541 ctacaaactc aatacatact attccatcat ttccacagca tcccggtgaa tgcctataaa
     3601 catagttaag agttttacca tcagcctcaa gagagttata taacccctct atttttatag
     3661 ttctaccaat ataatcatct gaattattaa aaatatcatc aagttgagct ataaacattt
     3721 tttctttaat ttctatagga gtcccatcat tagaagcaat ggaatctttt ttattttcta
     3781 ttttattagt atcgttacct ggatcaacta tatttttatg attttctact gttgctggag
     3841 tacttgagca cccagtaagt aataataaaa ataatactat tattaagttt cttactactt
     3901 tcatatgtaa ctcctattat tactttttat tttctctcct attatagata atataaaaat
     3961 aattatattt actactacaa tacttgcgcc tgtaggtgtt gaaaatttat acgatatagt
     4021 cataccaata caaaatgata atatagatac tagcccggaa ctaattacta cagacttaaa
     4081 acttttaaat attttcattg atattataga tggaaaaata attagacttg atataagcat
     4141 ggctcccatc attctcattc caagtacaat agttatagct gttaaaatag ctatcatcat
     4201 attataaaaa tttacattta taccagttga ttttgtaaag ttttcatcaa aggtaattgc
     4261 aaatatctta ttataataaa tagtaaataa aaatataact actattgata aaactacact
     4321 taaatataca tcatttttac tcatagctaa aatacttcca aacatataat tgcaaacatc
     4381 tgtattcatt ccagttgtta tagctattga tattactcca atagctagag aactggatga
     4441 tataagcgct atagcagaat caccttttat tttactactc tctttaattc tcaataataa
     4501 aaatgctact attataacaa taggtattga tagcttcaat ggtgaaacac ctagagctac
     4561 agctatagct atagctccaa atgaaacatg ggatagtcct tccccaatca ttgagtattt
     4621 ttttaaaact aaacttacac ctaataagga actacataat gatactaaaa ttcccactat
     4681 cacagctctt acaataaatg gataagacat catttctgac actaaattaa tcattaatct
     4741 caccacctaa aaactttttt ccacaatcac tctgcttata gtcttttaca tctccataaa
     4801 acaacattct atttttcata tgtactactt tattagcata atttaaagat gtttttatat
     4861 catgagatac cataataata gaaagtccgt agtttttatt taaattatgt agcatatcat
     4921 aaagatttaa tgtagttttt tgatctaatc cagttatagg ttcatccatt acaatcaact
     4981 tgtctgtggc gcataaagct cttgcaataa gaactctctg ttgttgtcct cctgataaat
     5041 cactatagca cttatttttt aaatcagata ttttcatttt ttccatattt tcatgagcta
     5101 gaactttatc tgattttcta taaaatggga atacactatt tctttttata cacccagata
     5161 atacaacctc ataaactgtt gctggaaagt ttctttgtat atcactttgt tgtggtatat
     5221 atccaatagt cgaatctttt aattcttcac ttatgaaaat actcccttta catggcttta
     5281 ctaaacccaa gattcccttt atcaatgtac ttttccctga gccattttca cctataatgc
     5341 ataaataatc cccttttgaa acagtaaaac taatatcctt tgctacaagt ttattttcat
     5401 atgaaaaact ggtattatta aactctacta agttcatact tattgtaaac cttccttcag
     5461 attacctata ttattgttca ttatatctat gtatgtaatt cctttatcaa aatcttcctt
     5521 tgtaacattg tgtgctgaat gtaataataa aactttagct cctgtttcgt cactaatagt
     5581 tttagctatt tttttattac tcatttcaat atgatatata actggaatct tattttctct
     5641 tactgttttt attaagtatg taactgtatc aatacttggt tctgtttgtg tactacatcc
     5701 gttaaaagca gccctatatg ttaatccata atcctttgca agatatctaa atggaaattt
     5761 atctcccaaa acaatcatct ttcttttagc tttagctact gtatttgtaa gtgatttatc
     5821 tagttcatca agttttttcg tataattttc atagtttgca ttgtaatatt tctcatttgt
     5881 attatctact ttaactaaag tatttttaat agcttctacc atattctttg catttttagg
     5941 tgatgtccag atatgttcat catactcttc gtcatgattg tgattgtcat tctcatcatg
     6001 tttatgtgat ttttccttat cgtgttcatg atcatggtca tgttcaactt caggttccat
     6061 accttctact tcctcttcct taacagcttt tacataatcc atcatcttaa tagcatttac
     6121 cttttttgta tctacagagt ctattatttt ttcaatccat tcctctgctt ctcctccatt
     6181 ataaataaat atatctgcat tttgaatttt ctttatatct tctggtgttg gctcaaaaga
     6241 atgactatct tgccctggag ataagatcat ttttacatct gccctatctc cagctatttg
     6301 ctttgcaaag tcatattgag gaaatagagt cgtaactatt tttaatttat cttttcttga
     6361 actgatatta tttttgtcgt tttctttact ttcagttcta gtacatcctg ttaataccga
     6421 caatattatt aaaattatag acattccaat aacagtaact tttctcattt tacactacct
     6481 ttctaactta tctctacagt tttcacatat accatagaaa acagtctttg tattattaat
     6541 tttaaagtta tgatctttta atatatgatt atttaaatct tctagataag aacattttaa
     6601 gtgaattagt tctttacatt ctgtacattt taagtgaaaa tgttgatggc aatcctctct
     6661 ttcacctata tattgataac atgatcctat accttcttca atataatatt ttcttaaaat
     6721 accatcatca gctaattttt ccatatgtct atatacagtt gttcgtccta ctttattacc
     6781 ctgctcactt aaatagttaa atacttcgtc tattgttaca tggctatctt catttgattt
     6841 taaataatct aagattatct ctttttgctt tgttttatat acagttttat tattcatgaa
     6901 taatacctcc tgaaattata ttattaatta ttataatatc tgtcaatacg aaattgaaat
     6961 tcaatttcaa attttaa
//
LOCUS       DAMJYC010000156         6898 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJYC010000156 DAMJYC010000000
VERSION     DAMJYC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.163"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            135..1877
                     /locus_tag="PC472_003720"
     CDS             135..1877
                     /locus_tag="PC472_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HDF2728995.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2047..2235)
                     /locus_tag="PC472_003721"
     CDS             complement(2047..2235)
                     /locus_tag="PC472_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2728996.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2232..2813)
                     /locus_tag="PC472_003722"
     CDS             complement(2232..2813)
                     /locus_tag="PC472_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2728997.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2981..3799
                     /locus_tag="PC472_003723"
     CDS             2981..3799
                     /locus_tag="PC472_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2728998.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4809..5630
                     /locus_tag="PC472_003724"
     CDS             4809..5630
                     /locus_tag="PC472_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2728999.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5940..6719)
                     /locus_tag="PC472_003725"
     CDS             complement(5940..6719)
                     /locus_tag="PC472_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2729000.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2579 a          914 c          881 g         2524 t
ORIGIN      
        1 acttttttga agttaaattt tttatattat ttataatctt atctatatct ttatgttaaa
       61 ttttcttaac atgcattttt tatatttaat atagtaaaat aattaataac tatatccatg
      121 attggaggat ttttatggtt aaatccaaga agaaaatatt tgtttttcta attttaattg
      181 cagtttttat tatatttgca tcatacataa gaaattatac taatgataca aaaaaagatt
      241 ctgttagagg aactattcct tttgaaaaaa tggaatataa gcgtcctaat atacagtcaa
      301 tttgcgaaaa cattaacaag tgcaacgaca aacttttaac agctaaaaca gctaaggaac
      361 aattaaattt atttaatgaa gttgataaaa tttatcaaga tttttacagt acacttacta
      421 tagcaaaaat tagaaataac atagattcat cagacgaatt ttactctaag gaatataagt
      481 atcttatgag taaatcagta gatgttgaca tgtcatacaa agactttcaa gatagatttg
      541 taacttctaa attctctaaa gaattagaaa aagaacttgg aaaggataca tttaattatc
      601 taaagaacat tggaaaactt aattctaaag aagttgaagg tttgcttaaa aaagagcaag
      661 acctagttgt aaaatatgaa gacttattag ctaaatctac agtatcaata gatggtatag
      721 aggtagactt tgaagaagcc ttgtcaagac caaatcttag ccctgaagaa tatgtgaaaa
      781 tttattcaga ttacttaaaa aaatacaatc ctatatttgg taacatattc ttagaactta
      841 tacagacaag aactgaaata gcaagtaagc aaggatttaa gaattatata gattatgcct
      901 atatgaacct aaacaaagac tattctcaag aagaagctaa aaaatttaga caagatgtaa
      961 aagattacat agttccatta tatagagaga tttcatctaa accatctgat tcttctattt
     1021 atattaaagt atataaaaat cgctcattta ggaagtttga taaagtactt gaagatatat
     1081 ctccaaaact aaaagagagc ttcgactata tgaaaaaata tgacttgtac gattattctt
     1141 ctggaaaaaa caaatcacct ggaggatata ctacttatat aaataaatat aaagcacctt
     1201 tcttgtttaa tacatgggat aattcttttt taggtgttac tagttttgct catgagtttg
     1261 gtcattttta taattactac aacagtatta acttaaacaa taagatgcaa ccaagtatag
     1321 atgtatgtga agttcattca acatctttag aaattctatt ttataaatat tttgatgatt
     1381 tttttggtaa acaatctgaa gctataaaaa aagaacactt atcgattgtt ttaaatacta
     1441 ttattgatgc ttgtctttat gatgaatttc aagaaattat atacaagaat ccatatatga
     1501 gtctaaatga tatcaataaa ttgttttttg atttagagga agaatatgga gtttccaata
     1561 atatacttag agaaaaaaat gctccttttt ggatacttgt atctcataac ttccaagttc
     1621 ctttttatta cttgagttat ggtcttgcat ctgatgtatc acttcagata tggcaattat
     1681 cacaagaaga ttacagaaaa gctgttgatg tatatatgga ttttttaaat caaaatactg
     1741 atgctggatt taaagatgtg gtagaaaagg taaatttaca attacctttc caagatggaa
     1801 atttagagga aataagctct actttatatg attattttgg tatagataat ccgcttgaac
     1861 taaaaaatgc ttcttaattt ctaatacttt atactcactc ttaagattta gtattagatt
     1921 gaaataagtg atattttttc acacacaatt aatgtttaat aaaattatat ggtaaagtat
     1981 cagaaatttt taagactctt gtctttataa tttctgatac tttatttatt aataatatta
     2041 ttatttttat atatatataa ttaaaaggat tgtggaaatc aataaaaata tccctgtaaa
     2101 aatagcacta ttcatatgga acttaataga acttgcatct gttaccttat tattcttaat
     2161 ccagaaccca caaatcatag ttgaaagaaa aagtaaaaca gtgataagac ttgaaccaat
     2221 agttataatt ttcatcttac atcaacatcc tctccaataa tattttctat taaaatatca
     2281 atgaatttat ctcgctcata tttattcttt atatttatac cactgtataa ctgaaatgat
     2341 tctgtagata aagcagaaat ctgtattggt aaaataattt gcagggcaat aattcttaga
     2401 ctcatttcat cttttttatt tccaaaaatt ttacgaagca tagggacaat atcaagttct
     2461 gcttgcatat ctccattggt tatacattga ttcaatacaa aaggaagcac tttttcataa
     2521 tgtaatccag tatcacacaa tttttttaat aaatttttta aatcttcaat aggtcttaga
     2581 gtatatacac tatcgtcata tagctctgct attatatttg acatcacatc tcctatggca
     2641 gtactcaaca aattatcctt tgttttaaaa tgataattta ttaaaccaac tccaacattt
     2701 gcacgctcag caatttttct aactgtaatc tcctcaatat catctacttc atttaaaaga
     2761 ttcaatgtaa cttcaattat acgttctcta gcatctttat cattactttt cataatagac
     2821 ctccaaaaca attaacattt tcagttctga acatgttcag tataatattt ttttcatcat
     2881 atgtcaatat aatttaatta taattttagt tgactctaac attatgatat ggtttatatt
     2941 aatattaagc taaaagtacc tttataaagg agacaaaaac atgtttaaga taggcgattt
     3001 ttcaaagcta agtaaaatta gtattcgtat gttacgtcat tatgatgaaa taggactttt
     3061 aactccttct catacgaata aaactaatgg atatagatac tatagtgcag accaactttc
     3121 tactacaaac agaatccatg cactaaaaga tatgggcttt ggcttgtatt ctataaaaga
     3181 aattttgaca gaatataacg ataaagaaag tctcataaaa tatctaaata tccaccattc
     3241 tcaagtaaaa gaacaattag aagatacaca gaaaaaacta ttaaaaatag aaaccactat
     3301 taaaagaatt ggaggaaatg atattatgaa aaattatgat gtaactataa agaattttgc
     3361 accaaaatat atgatgacac ttagaagggt tataccaact tatcaagacg aaggaatgtt
     3421 atggcatcaa gcatttttag agacaaaaga ccagaatgtt caaattgaac ccccaaagta
     3481 ttctaaagca gttttttatg atactggata taaagaagat tatgtagatg ttgaagtaca
     3541 agttgctgta tctggaaagt ataaagatac tgaacatgtt aaatttaaga ctgttccaag
     3601 tgtgactgct gcaactgcaa ttgttaatgg aaatttcaat caagttgcag acgcttgtga
     3661 ggctattggt aattggatat cagataataa ctatgatgtg gatggtccta tgtttaatat
     3721 ttatcatgta agtcctggca acgatagtaa tcctgataat tgggtaacag aggtatgctt
     3781 cccagttaaa aagaagtaat ttttatatta attggataat attttaattt gttagacaaa
     3841 ttacttttag tgttatgtat aaatacctac aatttaatta ggatattatc ttaatttgtt
     3901 aaacaaatta cttttagtgt tatgtataaa tatctacaat ttaattagga tattatctta
     3961 atttgtcaga taaatcactt gtgatgttat gtttgaatga ctagaattta atcaggatat
     4021 tatcttaatt tgtcagataa atcacttgtg atgttatgtt taaatgacta gaatttgatt
     4081 gggatattat cttaatttgt cagataaatc aattgtgatg ttgtgtttaa atgactagaa
     4141 tttgattagg atattatctt aatttgtcag ataaattatt tataatgtta tgtttaaata
     4201 cctacaattt aattaggata ttatctcaat ttgtcagata aatcaattat gattttatgt
     4261 ttaaatgact agaatttgat taggatatta tcttaatttg tcagataaat caattataag
     4321 attatatact atccttatac tctagttaaa tatattttaa tcactaatat aaattagttt
     4381 ttttaacaaa acaataaaag agtaaagtac aaataattcc tttgtactct actcttttta
     4441 actttctttg tacaataata caatgataaa tataaaaaat tatatattcc attctataga
     4501 aatatgctca ccaataacat gtatgttgtt aatcaatata ttatatcaaa ttatatcaaa
     4561 attacataaa ccttatagga caatcatatc ttattacaag aaaaatgtct tatccatatt
     4621 gaataagtca ttcagttcat atacaaagtc aatttcattg agaaacattt aatatatgtt
     4681 tataaaaatt tcatactatt ctttcatatt tgcatgtgat ataatttatt aactcataat
     4741 tttgttaatc agttgtgctt gtatattatt tatataatct aatattttaa gaaggtggtg
     4801 taataacttt gaaattaaaa attgttggta gtggcggtat gtcaattatt ccaagttcat
     4861 tttgcaaatg caaaatatgt gaagaagcca gacaaaaagg cggaagatat gaacgattag
     4921 ggccaagttt atatattgat gatataaaaa tgctgataga cacacctgaa gacattgcag
     4981 tagcttgtaa cagacaaaaa atatcggaag taaaacatat ttcaatttct caccatgacc
     5041 cagaccatgt taagggtatt agaattgtcg aaaaaatagg atgtgacttt atcacaggag
     5101 aaaataatcc aattggcttt tatgcgcttc ctgaagtaat tgaagatata aaccgaacga
     5161 atctcgattg tcttaaatat tatggtgatg ttataaattg catttctatt aatgaaactt
     5221 cgcatgtaaa gataaataat tttgatattg atttgattaa caataaccca gacaggaata
     5281 taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat atatgcctgc tgtaatccca
     5341 agccatttac tcatagtgat atgtactttg atgctgatgt tttaataata agtctagttt
     5401 ctgatgatgg tatattaaat gatggtacaa aacttaaaga cacaccattt aaagatgaaa
     5461 tatttacttt agatgagatt gttgaaatta aaaactatta tagaattaaa aagattatca
     5521 ttacacatat tgacgaaata tggggaaaat catatggtta ttacagtgaa ttagaaaaga
     5581 aacttgataa catttatttt gcatatgatg gtatggaaat tatagtctaa atcattaagt
     5641 aataagcata atgcaaaata agaaaacttc aatttaacag tatagttaaa ctatgtaata
     5701 tcaacatatc tcagttttta caaaatatca tagtatttat ctttataaaa tttcaagtcc
     5761 tacatatttt aatacttcaa tatctaaata aagtaaaatc aaaaatatat tttatataca
     5821 ttatagacgg cttatcggca ctagataagc cgttttgtca caattattat ttgctcaaca
     5881 aaacttctca taatatataa cagattgaat ctccaactga tacttaatta ccgaaactct
     5941 tattccttaa tatgttgttg cattaaccaa accttcatca tcattttttg tggcaatagt
     6001 tttgctaata aatgactgga ttttgtattt ataccataca cagaaatatc cttatttttc
     6061 tttgcatcct tcaatgcttt tttggcaaca atgtctggag acactatgcc tgggaaattt
     6121 tttgtccctt tttcagctac aactatacct ctcttaaata gatttgtact catccaacct
     6181 ggacatacag caattgcatt tatcccttta ctcttaagtt caacatttaa tgctcttgta
     6241 taatttctta taaacgcttt agttgaacta taaatatttt gatatggcaa tggctggaat
     6301 gatgcttgtg aagaaatatt catgatatgg cttccctttt gcatatatgg aatacatata
     6361 agtcccaatg ctacaacagc actaatattt aaatcaatca tattaataga ttcatctata
     6421 cttaaatcat tgtatgaaca gaattttgca aaaccagcat tgtttataag gtattttata
     6481 catacatttt ctttacttaa aaatgtacca atttctttta cttcttctat ttttgataaa
     6541 tcttttgaaa aaatttttac cttacttcca aattcatcta ctaattgatt taatctcgcc
     6601 ttgtttcttg caactgccca tatttctgtt atttcttctt catttattaa taactttaga
     6661 aactctttac caaaaccact attagcaccc gttataattg caatcttctt ataactcata
     6721 attattctcc tttaccataa gtttatacgc tcaaactgta aacttatttt ttacaaataa
     6781 aacaccctct attcagatgt tatattcata ttatttgaga tattatattt atatcatttt
     6841 caataattgt tatatagcat aaaaaatagt gttaatatta tagaatcggt attgatta
//
LOCUS       DAMJYC010000157         6838 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJYC010000157 DAMJYC010000000
VERSION     DAMJYC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.164"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..107
                     /locus_tag="PC472_003726"
     CDS             <1..107
                     /locus_tag="PC472_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2729001.1"
                     /translation="EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            234..1136
                     /locus_tag="PC472_003727"
     CDS             234..1136
                     /locus_tag="PC472_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2729002.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKDRGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            1748..2761
                     /locus_tag="PC472_003728"
     CDS             1748..2761
                     /locus_tag="PC472_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2729003.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQVNVY
                     IDN"
     gene            3113..3682
                     /locus_tag="PC472_003729"
     CDS             3113..3682
                     /locus_tag="PC472_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2729004.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(4018..5355)
                     /locus_tag="PC472_003730"
     CDS             complement(4018..5355)
                     /locus_tag="PC472_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2729005.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(5878..6588)
                     /locus_tag="PC472_003731"
     CDS             complement(5878..6588)
                     /locus_tag="PC472_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2729006.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         2545 a          716 c         1056 g         2521 t
ORIGIN      
        1 gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa
       61 atatattaaa agaaggatta agactaataa caattcaaaa taaataactt atgtaagaac
      121 cgtaggaact acggggatag cctgtggaga attagtaaga catatgttag tatgcaaaat
      181 tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattgga aatgtgttgc
      241 cacaagatta ttatggatta atagtgattt taggtgttat tcttggagtt atgggggcat
      301 tttacaactg gtttactcta tatgtgcagt ctttatacaa gaagattcct aaaattggta
      361 cctttggaaa actgttgatt ccatttataa tggctggtat tcttgggctt gttatgcctg
      421 atgttctggg gagtggacac aatttagttg aagaacttac aagtcatgaa tatatttttg
      481 gaatggttgt tttgattttt gttatacgtt ttatattttc ggctgttagt tttgggtcag
      541 gtgcaccagg gggaatattt tttccattgc ttgttttagg agcatttata ggtggaatat
      601 ttggtatggt aggtgttaaa gtttttggaa tgaatcccga ttatgtaaat aactttgtgc
      661 ttttggctat ggcaggatat tttacagcta ttgtgcgtgc ccctttgact ggtatcatat
      721 taatttttga aatgacaggt tctgttagtc aaatgctttc actatcagta atttcaattg
      781 tggcatatat tgtggctaca cttatgaagt ctaaacctat ttatgaaagt cttcttgaga
      841 ggttacttga aaaaaatgga gaacctgtac ataaagatag gggcgaaaag atactagacc
      901 agttctctgt tatgtttggc tctcctgttg cgaacaaggc aattcaggat gtacagtggc
      961 ctgaaaattg tcttttggta gcaattcaaa gaggtggaga agaaattatt cctagaggta
     1021 agacaattct tagaccaagt gatgtaattg tgactatgac agatgagaga gatagtggtg
     1081 ctgtgtatga taagatggaa aatttgtgta aagaacatac ggcacatatt ttttagggat
     1141 aatgatgaat gtttaataat gatagaatat ttaatgaaga tttgattaaa aaattggtaa
     1201 tttgaaagag agttaaggaa ttaatataag aaaatgaaaa tgtgatatct tagatgtaaa
     1261 agagtttgaa gaaaataatt gtatatattt gttaatgaga ttatatggac taatggtata
     1321 tttatactgt gctaaaagtt gcactattgt atgaggaatg ggaataaagg gtttgaaaaa
     1381 ataggtggaa gatttttcag atattcttat tgggatattt taattaatat attttaattg
     1441 cgatattttg ggaagaatag aatttattta taaatgaaaa taattaaaag gggttaaggt
     1501 tgtattagcc ccttttgctt atagagtttg gaaagggtat cttgatagta gttgtttcat
     1561 gcttattata ttttattaca tattttaaaa ttgaccttat tactcctaaa tcattgttat
     1621 atcaactttt aaaacacttt caagacaatc gcctattgaa aacaaagtta attaaaaacc
     1681 tacatatcag tttagtattc gtgtagatga ttatataaat ataaaagtat aagaaaggtg
     1741 agtatctgtg gttagttcat ttttaaatga tatctttaga acaattttag taagttgtat
     1801 ttttatagtt attttattag tctttagaat aactttattt aagactttca gtaaaaaatt
     1861 taattactat atatggctta ttgtgattat taagctatca ttaccattta tgaattatac
     1921 ttttatattt aatgagtcaa aatataatgg taatattaat aagataagtt taggcaattt
     1981 taatagtatt tctattgtat atagtactgt tttagtctct gtttggattg ctattacgat
     2041 attttattta gtttatactt tattgaaata tattaagctt aaaaatatga taaatgattt
     2101 gtcttatgat gttgaggatg ataagataaa aaatctttat gaaaatttat taaaagagct
     2161 taagataaat aaaaaaatac ggcttaaata ttcatatgaa gttgaaagtc cagtattttt
     2221 taattcgtgt gtcattttac caccatgtga ttatacatta aaagagttaa actggatatt
     2281 taggcatgag ttgatgcatt ttaagagtaa agacctttat attaagtatt tggtgatatt
     2341 tttaaaatgt gtttattggt ttaatccttt tgtgtatata atggagaagt ttatagattt
     2401 ggattgtgaa ttttattgtg atgaaagagt tttgaagaat tgtacgatag aagagaagca
     2461 ggattatgca ttgacaataa ttagtgcaat gagaaaaggt tcaaatagtt caaataaatt
     2521 tgtggctgga ttacataagg agtcagatat taaaaaaaga gtgtttagta tgtttaatga
     2581 aaagtacaga aatggtattt tgatagcagt tattttatgt ttattatctt ctattacata
     2641 ttttaaaatt gatttaatta ctactaaatc aatattttat ccactttcaa aacaaacacc
     2701 tattgaaaac agaactaatt caaaacctac atatcaggtt aatgtttata tagataatta
     2761 aaaaatacaa agtttaacaa gagattaata taaataattt taataagtat ctttcacata
     2821 tgttaaattt actttaatta tattaaagcc aatgaattat tctttagaag ctccaatgcc
     2881 agtaaataca ggtaaagaaa aaaatactac aattgtagat taaaaatgtt ctaatattta
     2941 aatagataca aaagtgtaag aagggtaaaa atctgttata atacattaaa aactaaattt
     3001 agaaaaattt acataattag aaaaatgatt tgataaaaaa taaagttaaa tattaactaa
     3061 gtaaatttga aaaagaaaaa atataaagta caaatttaaa aggaggtaac taatgaaaaa
     3121 gaagttggaa gatatgactt tagaggagtt atggaacttg tttcctatat ttttgactac
     3181 tcacaaagaa tgttggaaaa attggtttaa agaagaacaa tatagactaa ctggaatatt
     3241 gccaatgcaa aaaattgtta gaataagtca tattggaagt acttctgtga gtacgattta
     3301 tgccaagccc attgttgaca tactgattga gataattcct gattataata tgaaagatat
     3361 taaagatttg attgagtcga atggatatat ttgtatgtct gagagcagta gtaggctttc
     3421 acttaataaa gggtatacag tagatggatt tgaagaacga gtttttcatt tgcatataag
     3481 gtatgtaggt gataatgatg agttgttttt tagagattac ttaattgacc acccagatgt
     3541 tgcaaaggag tatgaaaaac ttaagttgga cctttggaag aagtatgagc ataatcgtga
     3601 tgcttataca gatagtaaac tagattttgt tagggagttt actgaaaagg caaaggtgtt
     3661 gtattctaat agatataatt agaaataggt acaattagat atttatctta tatatcgtat
     3721 tatatttttt aagaaagtaa ttaatagaaa aagggcttgt ttcaaaatgg agagataaat
     3781 ccatttttca ttgttgaaag gataaaaata tatttgattt catgtaaaaa agagtgcctc
     3841 atcaactaaa aagtttattt tgagacacac acatttttac taaggtaaat aaaatatcac
     3901 atttgattaa attttaagtt aatgaatata tgtgtaaata tttaattata agttattata
     3961 attttacata gtatcaaaca atctaatttg tattatataa gataatctaa ttttagacta
     4021 aaataatcta atataaagaa ataaagaaaa ttcactctca gtataagaaa tatccaattt
     4081 tccattgtac ttattaacaa tttctctaat attctcaata ccatatccgt gagaattact
     4141 atctttttta tctgaaataa atagttcttg ttttctattg ataggattta ccttgttgtt
     4201 tgtcaataaa tagctaaaat actccttctc acaacgtgcc tttaatacca cttttctatt
     4261 tgacaaatca ggaattttca aagatgcttc tatagcgtta tctaaagaat ttgaaaaaat
     4321 agtacataaa tctataggtt ctatacccaa gtgattgtct atatctatat taaagtgcac
     4381 atcaacacca ttttcttcta aacttgtatg acgaagattt aaaattgagt taagagcttg
     4441 gtttttgcaa aattgttttc cactaagagt acctatacct atttttaact gattaaaata
     4501 ctttttagct ccagcaatat cttcaatttc taaatacgat tcaatcattt gaaagtgatt
     4561 attcatatca tgtcgcattt tgcgtatttg aatttgttga ttttctagta agttatagta
     4621 tttttctttt aggtgatttg cttgtttttc tatcttcatc tttacatttt caatcaatgg
     4681 ctgtaaaaga agtatcacac cgatatttgc cagaattgct gacaagatga ttagataagt
     4741 gccataaggt aagttgccta agataatatt cttattacta cccataggga ttaaattagt
     4801 aggtggcaga ataatagcaa ttaatattga taaaaatgaa gatatacata ccatatcaat
     4861 aaaaatccat attttatttg ataagtattg ttttatattg accaatctat tctttaggaa
     4921 aatatgtatt aaaaaccaaa ataataattt aattagagca gaaattatct ggactattga
     4981 aagtattaaa atatactctt ttgagtgtat aagttcctca tatatagggt tgtttataaa
     5041 aagaaaatta agcccaatta taataggata aagtatcatg acaactgata ttataggaat
     5101 taatttgctt ttgctacata ttataagtag tataaaatat ccgaatagtg taaacaaaat
     5161 atttatgggg tcggaaagcc atattatcat ttgactaata atagagtagc ccaaaatcaa
     5221 tatgattttg aaaatccaat gattcctaaa gtctataaaa taggaagtca acttataaag
     5281 aaaaaaggct atgaaaatat tgattaatat tagaataaaa tttatgcttg tagagattaa
     5341 agaataagtg cccatttaat atacctttct attaattaag attctggaaa ctaagtttta
     5401 aaaaattaaa tcttaaaaat taaaattctg gaaattagat ttcaaagaat tgattttgaa
     5461 aaataaaatt ctagaaatta agcttcaaaa aattgagttt taaaagattg aaattttaaa
     5521 aattaagttt caaaaaatta aatttgaaaa aaactctaaa aactaagctt caaaaaattg
     5581 aattttgaaa gattgaaatt ctagaaactg agctttaaaa aattaagttt gaaaaaactc
     5641 taaaaactaa gcttcaaaaa attaaatttt gaaagattga aattctagaa actgagcttt
     5701 aaaaaattaa gtttgaaaaa aactctaaaa actaagcttc aaaaaattaa attttgaaag
     5761 attgaaattc tagaaattaa gcttcaaaaa attaaatttg aaaaaacaaa actctaaaaa
     5821 ctaagcttta aaaagttaag tttcaaaaat aaaattctag agattgaact tcaaaaatta
     5881 agattctaac atataccttg taaattttcc tacaacctct tgatattttt tacggcttat
     5941 aggaattttt tcattctgaa ctttggcata agtttgagtt atactatcta catggtttag
     6001 attgacaata tacctttgat gcgtacgtat aaaattagag ggaagaacag tttccatatc
     6061 atttaatttt ccgtagtatt ctaatgtttt atcaaaggta actacagtta attttcttag
     6121 ctcactttta aagtagagga tatcttttgt attaatttta caaagattag ttccaatctg
     6181 aactgtaata aagttttctt tccattgttc taattttttt agtgcttctt ttaatacttt
     6241 acatattttt tcagttttat aaggttttaa gatgtagttc aaagctccta catcatatcc
     6301 atgaaataca tagtcagaaa aacctgtgac aaagatgatt atagtatctt ttgaatgttt
     6361 tcgtatagtt tttgctatgt caacgccatt actttttcca agttcaatat ctaaaaaaat
     6421 aatatctatg taataatttt cgtgttctag attgtatact agactttctc catttgaata
     6481 ttcttttatt tggtaggggt agccccacaa atccatttct cttgtaatga ttttattcag
     6541 catgtttctt tgttgttttt catcatcaca aatacaaatc gaagtcaatt ttttatctcc
     6601 tttcaaattt tatatttata tagttaagta tataatatat tttgctacct agatatataa
     6661 aatgaaataa caattatatc cagatagcaa aataaaattt tttatattag tatacttaca
     6721 cttaaaacta ttctattaaa cttattctga agtgctaatg attttcttat taaaaaatct
     6781 aacaatcttt cttattataa aatacaaatg cttttattat tataaaaagt aatgattt
//
LOCUS       DAMJYC010000158         6771 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJYC010000158 DAMJYC010000000
VERSION     DAMJYC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.165"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            426..1700
                     /locus_tag="PC472_003732"
     CDS             426..1700
                     /locus_tag="PC472_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2729007.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKAFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1907..2224)
                     /gene="trxA"
                     /locus_tag="PC472_003733"
     CDS             complement(1907..2224)
                     /gene="trxA"
                     /locus_tag="PC472_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2729008.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2491..3243
                     /locus_tag="PC472_003734"
     CDS             2491..3243
                     /locus_tag="PC472_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2729009.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3230..4111
                     /locus_tag="PC472_003735"
     CDS             3230..4111
                     /locus_tag="PC472_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2729010.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4337..5692)
                     /locus_tag="PC472_003736"
     CDS             complement(4337..5692)
                     /locus_tag="PC472_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2729011.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2602 a          961 c          846 g         2362 t
ORIGIN      
        1 tacttgtaca tcttttataa catataaaat ctttttttct aattatatac aatatatttt
       61 ccattatgtt ctaatcaatt tctttactat ttttacaact ttattagtca tattcataat
      121 tatttctaat tagtgtttaa cattttatgt aaaatttata aaaaaatgta tataaattaa
      181 aataagtgac tatactaaaa atatgatacg gataaacaat aagttcaacc tatctccccc
      241 aaaatcaagt gagctacagt ttcccccaac tgtagctcat tttcgtgtaa tttaataaac
      301 atctcaacaa gaattaatta acaaaacccc ttataaaaac ataacatata ttaaacaagc
      361 tcttgattaa aacttgagct tttttaataa aaaacagaat agtttattag ataaggggtt
      421 tttttatgca attcatggat gtatctaaat acattgaagg tgcaaatctt aaagttaaaa
      481 ctaaaaatgg tgaaactgtt aaaagaatat attttaacaa ctctgccact ccattagttt
      541 taaaaaacgt agtggataac ttaaactgtg agattccttg gcttacttat attaatgctc
      601 caggaataat ctcagaaaaa aatactttaa aatatgaaaa tgtacgctct actatcctca
      661 aacttattga tggagacgaa gaaaaggatt ctgttattta tgtcaaaaat gctacagaag
      721 gaataaactt cttagcaaaa ttttttaaag atgaaaatcc agataaattt gttattacca
      781 cagcaatgga gcatatggct aattacctgc cttttaaagt taattttcct acaaaagttg
      841 ttggaattac agaaaaagga gaacttgatt tatgtcaatt agagaatata cttaaaaatt
      901 atgctggtaa tgtatctctc gttacagtta caggagctag taatgtcaca ggtataacca
      961 ctcctatata tgaaatagca agaatagctc ataagtatgg tgcaaagata ttggtggata
     1021 ttgtgcaagt aattcagcat aaagcttttt caatgatgcc acatgaatgt gatgagcata
     1081 tagattttgt tgtttttagt gctcataaat gttattctcc actaaatgga ggagctctgg
     1141 taggacctaa aaaattcttt gaaaaattta atcctgtact atatggctca ggttctacca
     1201 aatttgtaaa tgataacaaa atactatatg caaacgtacc ccaacgtttt gaagcaggat
     1261 acccagagta ttttggaact cttgcaatgg gaaaagcact gaacacttta aataaaattg
     1321 gtctaaacag aatttcaaaa tacgaaagag aattattttt atatgctaaa gaagaaatga
     1381 gtaaaatacc taatataata atatatggag ataaatcaaa taatgtaatt atcccaaata
     1441 tatcttttaa tataagtaat atgtattaca aagatgtagc aaaatattta gttgataact
     1501 ttggaataga aactggagct ggagtagtag gtgcagatat ttatgtacaa aaattattag
     1561 gtataaatcc aaaagaggca tacgtcagat ttatgaggga aaatcctgta ggtcttgtta
     1621 ggataagctt aggaatgtac aatacttttg atgaggtgga tagctttata catgctatta
     1681 aaactttggc ttttaaataa gatttttcta ttctttttta tcaataaata gagagtagtt
     1741 ttgttttcac aaaaaaacaa attatatcaa cagtcaattt cattagcaat tagacactca
     1801 tttgtaacta tacaaactat tttacactat acaaacaaaa aactcattac aatgagtttt
     1861 tttgtttata tatgagattt attttaatta cttcaattta ctttaattac atatatgatt
     1921 caattttgtt ttttaaattc tccttaggca taaaacctat taatctatct acaggttttc
     1981 catccttaaa tattagcata gttggtactg tagatacttc aaatttctga gctaattcta
     2041 tactttggtc tatgtccacc ttcaagaagt ttgctttttc aaccatctca tttcctaaag
     2101 cttcatatat tggtgatagc atcttacaag gtccacacca agtcgcaaag aaatctacaa
     2161 ctactaaacc atctttattt tctacttcat ttataaattc attattattt attattttta
     2221 ccataatata catctccttt attatcttct tgatattaat atactacgcc ctatataaca
     2281 gttcaattag gcactttttt gtaaccgcct atttttaata gatttaatat tatgataaat
     2341 agatttataa gattaaatat ttatgagact aaatatttct aatatatttt atttttttct
     2401 taatatagtt gacatttttt cccttaaatt ataaaatcaa attaataact acataggtag
     2461 ttactaatct tttatcaaag gagtttctat atggataata taattaatga attacaaaat
     2521 ataggattta ctaaatacga atctcaaata tacatatcat tattaaagga aagcccttta
     2581 acaggatatg aaataagtaa attatcaggt gtaccacaat caaaagtcta tgaaaatata
     2641 acaaagctat taaacaataa tataattatc aatgttggaa ctgaccctat taagtacata
     2701 ccaataaacc ctaatgaatt attaaaaaat aagaaaaaag aatttgacaa ttctatggat
     2761 aatctaaaga atagcttaca gatattaaat aaaagtaaaa gtgtagaata cgtattaaac
     2821 ataaaaggag ataaaaatat attaaaaaaa gcaatagaaa tgataaaaaa tgcaaaacaa
     2881 gaaattataa tttcttgccc ttatgaagaa attcttgaat taaaaaaaga actcctaatt
     2941 gcttacaaaa aaaatattaa tattgaaata ctacttttag aaaacaaaag tctgaaaggt
     3001 cttgataata ttcatattca tggaggtaat aaaactacac ttgaaaataa aatgctttac
     3061 aaaggaatta ttttgattgt agataatgat gaaatattga caggaaactt atcagaaggc
     3121 agtgaggcca tttcaatttg gacaaaaaac tctaacattt tatatattgg agttcaatac
     3181 ataaaacatg aaatttatat ttcagaacaa ataaaaggag gttataaaaa tgaacaaaat
     3241 taagattaaa caagttgatg cttttacaac tatacctttt ggtggaaatc ctgctggtgt
     3301 cgttactgat gcctctaaca tttctgatga aattaagcag aatgtggcta aagaaatgaa
     3361 tttatcagaa actgcatttg tttcagagtc aaatgttgca gattttaagg tacaattttt
     3421 cacaccaggt ttagaagttg atttatgtgg tcatgcaaca ataggtactt tccatgcact
     3481 tttggaagaa gggaagctta atactaataa gagtatattt tatcaagaaa caaaagctgg
     3541 tgtattatct gtagagctta aggaaataaa taatgaaaat atatttatga tggaacagaa
     3601 tacacctgaa tttgaaaatt tagataattg cagaaaagaa cttgcacaaa tgataggtgt
     3661 gcatgaagat agacttcttg aatatcctat tataaaggta aatactggac tttggtggct
     3721 tgtatttggt ctaaaatctt tagatgattt aaaaaatatc tctccagatt taaataaaat
     3781 caaaaaattt agtggtgata ataatataat tggtataact ccattttgta tagaaactat
     3841 agataaaaag tgtgactacc atttaagatc tatagcacca tatgttggag ttacagaaga
     3901 ccctgtatgt ggaactggga acgcttgcgt atcatcttat atagtacatc acaatatttt
     3961 agataaaact agttttattg gtgagcaagg taactttata aacagacctg gaaaggtata
     4021 tgtaaatata actagagaaa acaatgacat aacttctgta aaaattggag gaagtgctta
     4081 tactgtttta agtggtgaaa ttatgtatta atcccatacc taataaaaac aaataaaaat
     4141 actagctaac taaagctagt atttttattt gttattttta tttaataatc atactctaac
     4201 aaatattata attatatata tcataatatt gtatttagaa gtaaaatttt atttttatta
     4261 ttaattagtt ataaatcaat ctaatacaac ataattacag tagattaacc tataaaacat
     4321 aataattgct tactgattat ttatcgtcct caacaagtct acttaatgtc ttatcagata
     4381 agaataataa tatcgctgca acaaatagta ttactggaat tatagtatat actttaatca
     4441 tatcaaattt aagagtaaat gcatatatat atccgtatcc tttaccagct ataaaagtag
     4501 caaatgtcca tactcccata agaacaccta gaagcttctt aggtgcatac ttacttacaa
     4561 aagagttacc aagtggtgag aataacattt caccaagact taaaagaata ccaaacatga
     4621 ttatccatag tatactagct ttagcagttt caggagctcc aacccctctt gaaaattctg
     4681 caccaacaag cattaagaat gcaagaccta agaatattaa tccaagacct gtctttttaa
     4741 ataaactcat atctccttgt ggtctactag caagtttaaa ccataatgct ccaagaactg
     4801 gtcctaatac tatacataca agtgaattta atgagtcaaa ccaagcaaga ggaacatcaa
     4861 atcctccaac attcatgttt acaaatgctg caccataatc ataaactgca agataagtta
     4921 gataccagaa tacccagaaa attacagaga agaatgatat aagtataata gcccaaattc
     4981 tcttcttatc catgcttgta agaggagact tatctttttc ttcaattttt tcagcaacta
     5041 caccttcttt aaaaggtctc tttcctgcat tcccaaggaa tctccatcca tatatgaacc
     5101 atattgcacc tattacacaa agtacagctg ctataaagaa acattgagaa aatccaagta
     5161 caccattttt tgcaaatgtt ttaaggtaaa gaataccaac tgctatagta ccaataaaag
     5221 aacctatatt aacaaaagaa tattgtactg aaaatgctgt atcaagttct tcttgactat
     5281 caaaaagctg tccatttacc gcagatacgt tacctttaaa gaatgcagta ccaatcgaaa
     5341 ctaagattat cattgcattt accatagaaa ctgtagttgc aatagaacca agatagtatc
     5401 ctacccccat gataagcata cctataggta tagtatatct agctcctatc catctatctg
     5461 atatatatcc cccaattata ggtgcaaggt atgtgaatgc aacaaggttg gcttgcatta
     5521 tagcagcttg tcctttatct attccaagac caccatgaac tatagctgtt gtaaggaata
     5581 tatatattaa gtactttgaa gcataatatg ctgctctttc aaatgagaaa gttgttgagc
     5641 agatataaaa tcccatagga aatttaccct tcttttttac tgttgagctc ataattttaa
     5701 atcctttctt aattgtattt ttatttttta cgttttgtcc aaattctttc ttttatgaaa
     5761 gatataatat tattattttg taaaacaatt tgtaattatt attaagataa ttgcgcattt
     5821 atttgtagag tacatattct catggaataa aattaataca cttcttaatc ataaatttaa
     5881 attatataaa ttcactattt tcagatatag ttataaatta atatattctc atctaatacc
     5941 atgaaaaaac cttcgccata tgcaattctt attttttacg tgatagtatt ttacaaacta
     6001 aaatccccct tttgtagcaa cttctgtaat tacattttag tgctttttta aaggagtctc
     6061 gaataaagca acatatattt tgttcaaata atgtttttat ttacttttta aatttatata
     6121 ttacttcacc caaccaattc tcaacttaaa taaataaact actatctatt cacagattag
     6181 ccctatttct tacacattag tttataataa acactgtgaa tctaaacata aaaactgtta
     6241 ttttaaagta attacccata tattcgcttt gatactattt attgaggtta tactctttat
     6301 gtataatata ttatttataa aatacctcaa aatatattat tcataaaata cctcaaactg
     6361 aaaacttttt cacgaatatt tatttttcac ctcccctttt ttatttcacc atgtaaatcg
     6421 taccataaat atgcacttat atgcaagaaa aaaatctatt aatcgacagt aaataattag
     6481 aaaaattcaa attatattta tcaagtatat tatataaaat atttcctatg aaaatataaa
     6541 ttaaattaat gttcagaatt gtttttagat atgaaatctt gattttttta ttactttact
     6601 tatttgctca aatatatttt tgtatgcagt atataataat caaattatac acttcttata
     6661 actgcattga tttgaaaata atattaataa ttaatagaat aaaaaattta aattaaattt
     6721 aaaaaaattt gttaagttga ttgaatactt ttttatgtat ttaattaatt t
//
LOCUS       DAMJYC010000159         6700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJYC010000159 DAMJYC010000000
VERSION     DAMJYC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.166"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            199..1032
                     /locus_tag="PC472_003737"
     CDS             199..1032
                     /locus_tag="PC472_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2729012.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1125..2057
                     /locus_tag="PC472_003738"
     CDS             1125..2057
                     /locus_tag="PC472_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2729013.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2109..2591
                     /locus_tag="PC472_003739"
     CDS             2109..2591
                     /locus_tag="PC472_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2729014.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQESMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2603..3472
                     /locus_tag="PC472_003740"
     CDS             2603..3472
                     /locus_tag="PC472_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2729015.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIRAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3515..4270
                     /locus_tag="PC472_003741"
     CDS             3515..4270
                     /locus_tag="PC472_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2729016.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVNKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4312..5133
                     /locus_tag="PC472_003742"
     CDS             4312..5133
                     /locus_tag="PC472_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2729017.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5138..5899
                     /locus_tag="PC472_003743"
     CDS             5138..5899
                     /locus_tag="PC472_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2729018.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKINPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIFVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6060..6317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6430..6669
                     /locus_tag="PC472_003744"
     CDS             6430..6669
                     /locus_tag="PC472_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2729019.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2951 a          627 c         1139 g         1983 t
ORIGIN      
        1 atataatact caattttcaa cattctatac aattatacag aaattataca gcataataat
       61 caccgaaata acaaatttta aaaattaagc aaaacttttt gtcgaagttc aacgtctata
      121 tatatgtagt gtaaattttt gggataaaat actacaaaaa tataaatgta aaaatatagt
      181 aatattggag gtcgaacgat gagaaataaa aagaaaaaaa taaatagaaa gaagttgtat
      241 ttgcttttaa gtgctattgt agtgtgtctg gcttttatag tagttggtgt tcgcgtcagc
      301 tctttaaata tgaaggaaaa tgaaattagt agaaatgcaa aactgtcaag ttctactatt
      361 acagatatag tatctaatac atcaatagaa gttagtaaag ggccaagtaa aagttctgga
      421 aaaatagcat acataactat agatgatggt ccatctaaat ttactgacga gatgataaaa
      481 actttaaata aatacaatgt taaagcaact ttttttatga tagatgggaa tatgaaggaa
      541 tatccacagc aagttaaaaa tataattaaa aatgggaaca cagcgggttt tcatagtgta
      601 tcacatgata ttcacaagct ttatgttact agtacatcag caaaagagga atttgataca
      661 aatgatcaaa ctttttataa gataactggt aaacattcaa aagtaataag aataccttat
      721 ggaagcaaac catatacacc acaagcctct tatcaggctt tagttgatgc tggatataaa
      781 atttgggact gggacttaga tacagaagac tggagatcta attcttctca aattgtacaa
      841 aatgtaaaaa atcatattaa gaatagaaaa ggtgaagata aagaccaact tgtagtttta
      901 atgcatgaga aaaaacagag tgcagaagct ctagattcag ttttaaaatt cttatcagat
      961 gaaggttatg aatttgcgcc agtagatcaa aaccaaatac caaaaaatta ttggttacgt
     1021 aatttagagt agttaaaata taagaatatt gattgatatt tttagaaatt gagaataaaa
     1081 atgagtgagg tgtaggtata ttgaataaaa aaatttatat aggaatggtc ggaatattgt
     1141 ctttgatgat ggttggttgt aataaaagtt tagcaaaagt aaatgatgtg gaaatcacaa
     1201 aagaacagta caaaaaaaca aaagcagttt tatctgctac taataattat ataaatgggc
     1261 agtctttaga tgaattagaa aaaactttgg ataaaaaagg tagaaataaa ttagaaaatg
     1321 ttataatatc gtttatggtg gataatgaac ttttatatca agaggctaag gataaaggac
     1381 taactccaag taaaagtgaa gtagactcaa aatatcaaga actagaagat aagatgaatt
     1441 tgaatacaag ctataaggaa aaaatggaca aagcaggtgt tgataaagat tatttaaaac
     1501 aagaaatatc tagagattta gcaatagata aaaataaaaa ggcctttgaa gatagaataa
     1561 acataagtga taatgatatg gaagcctact atacaagtca taaaaaagat tttaatgtag
     1621 aagaagtaag tgcttctcag atacttatat cgactttaga taaaaataaa aaagaagtaa
     1681 gcaaagataa aaaagaagct ttgaagaaga aggcagataa tatattgact aaaatcaaaa
     1741 atggagaaag ttttgaaagt ttggctaaaa aatattcgga tgataaagca actggaaaaa
     1801 atggaggaca gctaggatat tttacaaagg atgataaaaa tgctgagttt acaaaggaag
     1861 tgttcaaatt aaagaaaaat gaagtttcaa atgtgtttga aactagttat ggatatcata
     1921 tagtcaaagt tactgataaa agagaaagac aaaaaagttt taatgagtgt cagagtttaa
     1981 taagagaatc tattttaaat gaaaagtata ttgagcatat aaaaaaactg aatgaagatg
     2041 ctaaaataga cagatagaat ttctaacagc tcaatcctag cctgtaatcg gagggagatt
     2101 ttacaggaat gtttaagagt ttaaagagaa cgaaagttga gaaatacatt atcgataata
     2161 aagactcttt ttatagaata gcatatagtt atacgaagaa tgaagaagat gccttagatg
     2221 tggttcaaga atccatgtat aaggcactat actcagtgga aaatattaaa gaagtaaatt
     2281 atataaagac atggttttat aaaattttag ttagaacgag tatagatttt attagaaaaa
     2341 accgtaaata taataatatg acagatattg accttataga tgagactgga gagtatgata
     2401 aatatactga tttggattta agaagagctt tggaagaatt acctattgaa tacaaatcta
     2461 ttataatatt aagatttttt gaagatttaa agattgaaga agtagcaata atattggatg
     2521 aaaatgtgaa tactgttaaa actaggttat atactgcttt aaaaaaacta aaattaaaaa
     2581 ttgaagagta gggataaaaa gaatgacaag caaagaaagg ttaaataagt taaaagatga
     2641 gtatcataaa attccaatac ccaagaaatt agatactata ataaatcatg aaaaaataga
     2701 aaacatatat cgagagaaaa ataaaaaagt aaatagatta agatttaaag ttgcaatagc
     2761 ctttgcatgt atatttacag tattagtaaa tataagtcct gtatttgcag acaatttttc
     2821 taagatacct gtaattagag caatagtaga agtaataact ataaaaaatt atagtttaaa
     2881 aagtgaaaat tatgaagctg agattgatat tcctaaaata cgaggtttaa aagataaaaa
     2941 cttagaacaa agattaaaca gtagttttat ggaagatgga aaaagacttt atcatcaatt
     3001 ccaagagaga atggagaaga tacaatcatc aaagaataaa ggatataaat ctttaagttt
     3061 gagttatagt gtaaaaaata attctaaaaa atttctatca atagagatga caaaaaatga
     3121 gatagaggct tcatcatatg tgagtaaggt acattatact attgataaaa aaagacaaat
     3181 agttttgact ttgccaatgc tttttaaaga tgataagtat ataaaagtta taagtgataa
     3241 tataaaagaa caaatgagag aacaaatgaa aaaggatagt acaaaatcct attttataga
     3301 tcaaaaaaaa gacttgcctg ttgaagattt taaaacaatt aataagtatc aggattttta
     3361 ttttaataaa aatgaagacc ttgtaatttg ttttgatgaa tatgaagtag cacctggtta
     3421 tatgggcgca gttgagtttg taataccata taaagtaata aaagatttgt aaattgaata
     3481 gtagtttata tataaatctt ttaaggagga gaatatgact acatataaaa aacttaaaaa
     3541 atctatattt aaaattgcta tattttcaat atgcttatta gtttcattag tagatacaaa
     3601 ccctatattt gcaggtagtt cctttaatga aacttcaaat agtgatacga taaaggtgtt
     3661 aaatgctaag agtatcaact ttaaacaaga caattatgaa gtgaatgtga gtattcctaa
     3721 aataaaaggt ttaaaagata aaactttaga gaataaatta aacaatgagc ttttgcaaga
     3781 tgggaaaaaa atttacagtg aatttcaaaa taaaatgaaa aaggaagata ataagggtca
     3841 tagataccta aatgtaatcc ataatgtcaa aaataattct aaggactttt tatcaataga
     3901 aatgacaaaa gaagagatag aagcttcctc atctataact aagaagcatt acactgttaa
     3961 taaaaataga ggaatactat taaatttacc aatatttttt aaagatagta gatatattag
     4021 agctataagt gataatataa aagagcagat gagaaatcaa attaagaaag atgatacaaa
     4081 atcttatttt atagaccaag ataaagactc gccagtgaaa gattttgaat ctataaataa
     4141 gtatcaagat ttttatttta ataaaaatgg aaatcttgtg attagttttg atgaatatga
     4201 agtagctcct ggttatatgg gagcagtaga atttgtgata ccaaataaag taataaaaag
     4261 actaagatag atatatagaa gatttttaga tagactggta aggagaatta tatgaatata
     4321 tatgaacaag caataagatt aatagaaaat aatgaggatt tcgcatttgc tacaataacg
     4381 tctcattcag gttcaacacc acgtgaaact ggagcaatga tgattgtgaa aaatgattct
     4441 actatatttg gctcagtagg aggaggtagt gtagaagcag aatgtataaa acatgcaatt
     4501 aatgttataa aaaacagaga atcaatgttg tataaattta ctctaaacaa atctgatgtt
     4561 gcaaaactag gaatgatatg tggaggtact ggagaaatac aaatagattt tatagatagt
     4621 aaattaaaaa gtaatataga aaaatttaat aagagactaa aggaaaatac aagtaaagca
     4681 tatatatttg gtgcaggtca tatttcaaga gatgttgctg tgatactatc tcttttagaa
     4741 tttagaacag tagtaataga tgatagagaa gaatttgcta atcatgaaag gtttccagaa
     4801 tcagaagtag tagttttaga ttcttttgaa aacataccag attttccaac tgacgaaaat
     4861 agttatataa ttattttaac aagaggtcat ctatatgatt caagtgcttt agagtgggct
     4921 cttaaaagag aggctggtta tataggtatg attggaagta gaacaaaaat tggattgact
     4981 tacgaaaaac tcatgaaaaa aggttttaag aaggaagaat tatcaaaagt acatgcacca
     5041 ataggcataa aattgaatgc tcaaacacca gctgaaatag ctgtatgtat agctgctgaa
     5101 cttataaatt gtagagctaa taaggaaaaa taaactaatg aacacaggga gtaaagatat
     5161 tgatacttta aaggttaatt ttagagggat tgaaataaaa gcagttttag tatataaaaa
     5221 tagaaaaaat atttctataa aaataaatcc atttggcaaa ataactgtaa tgagtcctcc
     5281 taaaatatcc aaaaaaataa taaaagatat aattattgaa aaaggagact ggatacttaa
     5341 aaagtcagaa aaatgtaaag gaagagagga agtatataaa caaagaatgt ttataactgg
     5401 cgaaaaattt ctttatttag gtgaagagta ccatctagtt ataaaagaac tgttgagaga
     5461 ttctgtcaaa aaaagtaact gtagaataac tattaacgaa tatcaaatat ttgttcagac
     5521 aaatgataca agtacagatt ttattaaaaa atctttaaaa tcttggtata agatggaaag
     5581 tgaaagaata gtattagaga gaatagattt tcttaaatta aattgtgaaa taatgaagca
     5641 attagttcca gcgagtgtta aggtaaaaga acaaaataag agatggggaa gttgtacagc
     5701 tcaaaaaaat atttatataa actcaaaaat atctatggca agattagatg taattgatta
     5761 tgtaataata catgagttta gccatttagt acatatgaat cactctaaaa aattctatga
     5821 tttagtaaaa tccattatgc ccgattataa agataaagaa aattggctaa aaataaatgg
     5881 atataaaata ataatctgaa aacgattatt ccaaaatata ggcgtaaagt ataaaagaag
     5941 tattattgtg aaaaaaataa ataatctgaa aattttaaaa aaagtcattg acattgtaaa
     6001 tgatagggaa tataatctat attaacaaca aaacagaatt aaaatataaa attaaaaaat
     6061 taaaagcaat gacagagaca acaatactaa tagtgttatc cacagagaac caatgttgct
     6121 gagaattggt gttaacagta gtataatgat cactctggag cttttgtact gaatattttt
     6181 agtaagtaca aacgtatgtc aacgttacat gactaaggtt aaggtaatta attacccgac
     6241 ctgaaaaaag aggggtagat gtatttttta tatctacaaa taaggtggta ccgcggaata
     6301 taactttcgt ccttataagt aatatttata ttgcttataa agatgaaagt ttttttattt
     6361 taagcgatat aaataaattt aatccatata aaattaataa aaaaataata aaaataagga
     6421 gggattttta tggaaaatac tgtatatgct aaattaaacc aaggaataga ttctttttta
     6481 agagtggcta tgactctaag aagaagagag gttgatatac aatcaataag tatgactgta
     6541 gataatatga ataattcagg tataaaatta atagtaaatg aagaaaagac atctttagat
     6601 tctgtcttaa accatatgaa aaaactacat gatataagag agataactgt tgaaagaatg
     6661 agacattaat taaatatatt ttataaaact aaagataaat
//
LOCUS       DAMJYC010000160         6635 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJYC010000160 DAMJYC010000000
VERSION     DAMJYC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.167"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(348..839)
                     /locus_tag="PC472_003745"
     CDS             complement(348..839)
                     /locus_tag="PC472_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2729020.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
     gene            complement(2019..4154)
                     /gene="rnr"
                     /locus_tag="PC472_003746"
     CDS             complement(2019..4154)
                     /gene="rnr"
                     /locus_tag="PC472_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2729021.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            4750..5364
                     /locus_tag="PC472_003747"
     CDS             4750..5364
                     /locus_tag="PC472_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2729022.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDGSEYLRKS
                     VGNALRNISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            5382..5744
                     /locus_tag="PC472_003748"
     CDS             5382..5744
                     /locus_tag="PC472_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729023.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            5850..6347
                     /locus_tag="PC472_003749"
     CDS             5850..6347
                     /locus_tag="PC472_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2729024.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         2256 a          975 c          744 g         2660 t
ORIGIN      
        1 tttatgaagt tataatttaa tatatattta ttttatttgt gagtctattt ttatatataa
       61 tgaaaaatgt gataagtatc caaaagaatt actggatata ataagatagg gtatttagcc
      121 ttttaagata aataaaaata tctgcaacta tatattgcaa aaatttaaaa ctaatttata
      181 tatgctaata taaatattta taaagacata agacaaattt acctacagat ttaaatttaa
      241 cttatatatt ttaaaaggct aatataatat gtatttcttc tatacaatca aaaggcttca
      301 tatctaagat atgtgatgac actaatattc acaattttta gtaattatca cattataaaa
      361 aatattacag ctagtccatc attagatgct tcttgtataa actttttaag ctcaattaga
      421 tttgcccata catattcaaa aaaatcatct tcactctcat cattcataac aggataaata
      481 ttattttcta acatctcatt aatattaaat ctttttctaa atgcttgttc ttccaaactg
      541 ttcattgctt ttgatagttt ttttacatca tttgctgtaa tcaacatgac aggaaattct
      601 ccatcaattt ccataagtat gtttccactt agtactaatt tgctaaatat attatcatca
      661 tctccaccaa aaggacaacc tgtcaaagta aagtgaatgg catgccatgc tttatcaata
      721 ttgatagtac tattttcgtt ggcatcttca aaaacaaaat cttgtaaatt ttcttctgct
      781 tgctgtaatt taaaaacatt ctcttcactt attttagtat aacatcctat catccccata
      841 attacattcc tcctaagtag cgttttttta attgtaactt ataattaaac ttagttacaa
      901 taaaatatat tgagaaaagt attatttcat cttaaattta aatacctata ttatcaacta
      961 aaattctgtg tttatagcgt aagtattatt attttttcct aaaaattaaa tgaatcagaa
     1021 gttctatagg caatattgac tataaataag aactattact ttttaaaaaa aatttaagaa
     1081 attatactgt agaatcatgc tctacttatt agctttaaat aaaaaataaa tcatgttctc
     1141 aaattaaata ataaataaca ataaatttaa attttaaact ataagagttt ctggagtgtt
     1201 aataagcata ccaataatta tatgtgtaag aatttgattt aatagatatg aataattttt
     1261 acattaatta taaatatatt aacctattgt tataaattct aaaaaagaaa agttatctta
     1321 aataaaaaat aagtgtatct taaattttaa atgatacact tatttttata ttgaaattat
     1381 attttttaat atctattctt aattaggtat ttttaaatta tctgtttaca ttaaatatct
     1441 cttaattact tatctttcag ctagcttctt ctattaaatg tttctcaatt acttattttt
     1501 caattagcca cttctattaa gtatttctta attacttatt tttcaattag tcacttctat
     1561 taaacatttc ttaattattt atttttcaat tagccacttc tattaaacat ttcttaatta
     1621 cttatttttc aattagccac ttctattaag catttctcaa ttacttattt ttcaattagc
     1681 cacttctatt aagcatttct taattactta tttttcaatt agccacttct attaaacatt
     1741 tcttaattac ttatttttca attagccact tctattaaac gtttcttaat tacttatctt
     1801 tcagttagct ctttctatta aacatttctc aattacttat ttttcaatta gctactttca
     1861 ttaaatattt cttaattatt tatctttcag ttaactattt ccattagata tttctcaatt
     1921 acctattttt atcatttatt ttatagagta ttgtgcacta tatatttata catatcataa
     1981 atactacaac ttgttatctc ttattttagc tgttttatct actcctctat tttttcaact
     2041 atctcaaagt caatttcttt aaaatctaca ttaacacttt caacctttat cttaacaagg
     2101 tctcctatct tatatgactt cttagttctt tctcctaaaa cactataaga actctcatca
     2161 taaatatagt aatcatcatt catatttgca agtcttatca acccttctat agtattatct
     2221 aactcaacaa atattccaaa tgaagttacg ctagatacaa caccctcaaa ttcttgacca
     2281 actttatcct tcatataaag agccttatag aaatcatgta catctctttc agctaactct
     2341 gcctttcttt ctttctcgga tgattgaaca gatgcataat ccactatagt agtaagctgt
     2401 tcttgtcttt ttccacttat tttattattt aaagattctt ttattattct atgaatttga
     2461 aggtcaggat atcttcttat tggtgaagta aagtgacagt agaatttagc tgctaatcca
     2521 aaatgtccac tacattctgg tgagtactta gcttgtttta aagaacgaag cattatagta
     2581 ctgatagctc cttcttcttt tttgcccttt attttctcta ttataccttg taatgctttt
     2641 ggatgtactt cctctttatc accttttatt atatatccaa atgtagatat aaacttactc
     2701 aattcttcca ttttttccaa agatggtgtc tcatgtattc tatacacaaa tggtattccc
     2761 atccagtaaa aatgttcggc aattgtctca ttacttataa gcataaattc ttctattatc
     2821 ttattagata ttcttctttc atatggcttt atatctacaa cttcgccttt tccatttaga
     2881 attatctttg cctctgggaa attgaagtct atagctcctc ttttttccct tcttgacata
     2941 agaatcctag ctaaaatttc agcattttta aaatcttcaa caacatgttc aaaagtcttt
     3001 ttaagttttt catcatcctt ttctaatata tcagatactt ctgtataagt cattctagct
     3061 ttggaattta ttacagattc acatatttca tgtttaacca catttccttt atggtctatt
     3121 tccataaata tagaaagagt tagcttatct tcaaatggat ttaagctaca tactccattt
     3181 gaaagagttt ttggcaacat tggaattact ttatctacta aataaactga tgtagctctt
     3241 tttaaggctt ccttgtcaag tttatttttc tctcttacat aatgtgtaac atcagctatg
     3301 tgaactccta gtttgaagtt tccattaggt aatacttcta ttgatatggc atcatctaag
     3361 tcttttgcgt catctccatc tatagtaaat atgtttaaat ctcttaaatc tcttcttctt
     3421 tttatctctt cttgaggaat ctcaacagct acagcttctg cttcttgcaa tactttcttt
     3481 ggaaattctt ctggaagacc atgttctctt attatagaat ctatttctac gcctctttca
     3541 cctttttgac ctagtatctc tataattttg ccttctggtt ttctatcctc ttgtggccat
     3601 actgtaattt cacatactac cttatcatta tctactgctc cactaaaatg ttttttaggt
     3661 atatatatat ctttagtaaa tttcttgttg tctggtgtta caaatccaaa agttttactt
     3721 gattggaaag tacctactat tctagttatt tctctcttta ttacttttac tattcttcct
     3781 tctgctctct tttcttcagt tgcaggtact actatttctg ccataactct atcattatgc
     3841 atagcaccat ttatattgtc acttgagata aataaatcct gtgtatattc aacatcagat
     3901 tctacaaatc caaaaccctt tttgtgtgat acaaattttc caacaaagta tcccatttga
     3961 ttgggagata ctatttttcc ttttttagta aagcagatat aaccatcttc ttctaattca
     4021 tttaaaaaat tataaaacat tggcatttct gatgaatgta tatcaaatat ttctgctaat
     4081 tcttcttttt ttagaggatt atatgcactt tcatttatta atcctagcaa actttcttta
     4141 agtcctggta tcatttttcc ctcctgtttt acttttcatt atattattat ttatattgtc
     4201 ttttatattt cttcagacaa aacatttttt acttatcaca taattttcta tatttcctta
     4261 ttattattac aagttaaaat tacttattta tatttttgat ttaatgtaag ctttgtcata
     4321 ctaacttcaa acaaattatg atgttttata aattatttgt aaatatcatt ataattactt
     4381 aagttattct ttatcataat tgcttaaatt actctttatc acctaattat acaccatcaa
     4441 ataggattgg taaaatattc atctcatata agtattacta tataataatt tattctatta
     4501 gtatactcaa atcatgtaaa atttatataa ctcaattaat tttttaaata caactttata
     4561 atataaaact tataataaaa gtaaatttat atgttaaatg aggttgattt tttattattt
     4621 tttatttatg ctagtactaa agacttgcaa acgttttttg acaagtcttt attttgtcta
     4681 aattaaacaa acttttttac aaataaatac ataaaattta aactaatcta tttattggag
     4741 gagataaaat tggaagaagt aattgcaaaa attattacta tagaaaatgg attcaaagaa
     4801 attgaaaaca ttgctaaaaa aattgtaaaa aataatgact caaaaaagtg ttattatcta
     4861 tcaatctact tatataggtc agaatattat caagtaagag aattggctgt gtttatactt
     4921 ggttttatat cagtaactat ttctgaagct ttgttatttt taaaagataa tgtgagtaaa
     4981 gatgaaaact ggagagtaca agaaatacta gctaaatcat ttgattatta ttgcagtgaa
     5041 gtaggatatg aaaaggctct tcctgttata aaagaatggc ttaattctga tagtccaaac
     5101 ataaaaaggg ctgtgacaga aggtcttcga atttggacta gtagagacta ttttaaatct
     5161 aatcctgacg ttgctatatc actactttct agtttaaaag aagatggtag cgaatacttg
     5221 agaaagtctg taggtaatgc tcttagaaac ataagtaaaa aatatccaga tttagtaaaa
     5281 aaagaattag attcatggga tatatcaaat aaaaaagttg agtttgtata taagcttgct
     5341 tcaaaatgta tcttggcaaa gtaaaatgga ggtgaactta tttgaaactt cgtgatgagt
     5401 atacttgccc attagaacta acacatgata tgataaaagg caaatggaaa tctattatat
     5461 tatggagatt acgcatagga ccaacttcct tatcaaaact tgaaaaggaa atcaatggta
     5521 ttaatcaaaa gatgttactt gaacaactta aagaattaat tgaatttggt cttgtagata
     5581 aaaagacata tgaaggttac ccacttaaag ttgaatactc acttacacct attcgaggga
     5641 aacaaatatt ggatgctttg gctataatgc aagatatagg aattagcttt atgttggata
     5701 atggtatgga agatattctt aaggaaaaag gtgttttaaa gtaaatagat acccacaaaa
     5761 aagtaagtaa tttaccatta ttttcaactg gtatataatt aaattataga tatattctat
     5821 taatttatta taataggagg taaattatta tgaaagtaac tagtacaggt atagtaaatg
     5881 gtgttataga agataaatac ggtaaaagag gaactcagtt caatgaaggt ggtatgccta
     5941 catattcact acctctaaag tttgaagatg ctccaaagaa tacagtatcc tttgctgtat
     6001 ttttagaaga taaagactct gttcctgtat gtgggtttgc atggatacat tggttagctg
     6061 taaatataac aaaagatgag ttaaatgaaa atgaaagtgt aacagctact gattttgtac
     6121 aagggactac tagttggcat ggcaaactag gaggtcttga cagattagag gccagtgtat
     6181 atggtggaat ggctcctcca gacagacctc atgtctatga aatacatgta tttgcacttg
     6241 atactattct tgaccttgaa aaaggattct atatgaatga atttttcaaa gctattgaag
     6301 ggcatgtttt agatacattt acactaaaag gaagttattc taattaatta ggttgactat
     6361 agctaatcat taattaaatg ctacatttaa ttgatgatta gcttaattgt tattagaata
     6421 ttttctgcac tttatcttaa tatatatttt agttgactgg tattttaatt tatattagat
     6481 tattatcaat ttatataaat tgattttagc ttaaaactta cttatctatt tcagtttcta
     6541 ttagaattat tattagactc attaaaataa aagcactaat ataactaata atatatgtta
     6601 aattcattaa ctctacataa agttttataa tctaa
//
LOCUS       DAMJYC010000161         6545 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJYC010000161 DAMJYC010000000
VERSION     DAMJYC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.168"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            88..546
                     /locus_tag="PC472_003750"
     CDS             88..546
                     /locus_tag="PC472_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2729025.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(663..1400)
                     /locus_tag="PC472_003751"
     CDS             complement(663..1400)
                     /locus_tag="PC472_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2729026.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            1617..3293
                     /locus_tag="PC472_003752"
     CDS             1617..3293
                     /locus_tag="PC472_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2729027.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3500..3700
                     /locus_tag="PC472_003753"
     CDS             3500..3700
                     /locus_tag="PC472_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2729028.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            3754..4917
                     /locus_tag="PC472_003754"
     CDS             3754..4917
                     /locus_tag="PC472_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2729029.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(5128..5550)
                     /locus_tag="PC472_003755"
     CDS             complement(5128..5550)
                     /locus_tag="PC472_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2729030.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(5571..6407)
                     /locus_tag="PC472_003756"
     CDS             complement(5571..6407)
                     /locus_tag="PC472_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2729031.1"
                     /translation="MRQEERTVCFDRELKIEAYTFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
BASE COUNT         2491 a          787 c         1068 g         2199 t
ORIGIN      
        1 ttatttaaat gtcttgacaa aaaatgaatt atcaattaca atattattta ataaagatta
       61 tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa tggatttatt aaaagacaag
      121 ttaaaagaaa ctggttttaa gattacgcca caaagaagag caatagttga aatattgctt
      181 aaacatgacc attcacatct aagtagtgag gaaatctatg atttagttag ggttgactgt
      241 cctgaaatag gcttagctac tgtatataga acaatgcagc tattggatga aataggatta
      301 atatcaaaac taaacttaga cgatggatgt ataagatatg agattagtct acataaagaa
      361 gactgtcata atcatcatca tcttatttgt aaaaattgtg gaaagataat ggaagctaaa
      421 gaagaccttt tagacaatat agaaaaagag atacaatcat tgtacaaatt taaaatactt
      481 gatcatgatg ttaaatttta tggactatgt gacgagtgta atggagtaag cgattcagaa
      541 gaataaactt ctattgctta atgtcgatgg aagaatagta tagaactttt atattctatg
      601 ctattcttcc attttttatt ataaaccatc tataaaatgc aaatatacag ttttcaagat
      661 gcttatagtt ttaatattga aaatataatt aatcctataa gtacaaaaat gaatgatttt
      721 tctatgaaat tactaaatgg tcctccaaca gttaaaaaac ctaaagaaat tttcttttcg
      781 cttagcggat aaaaaattgg tattccttgc tttgtaaata aatctcctaa aaacatatga
      841 cttgcataac caatcgtacc ataaaaagat aaattattaa tattagtgat taattctatc
      901 tgttttagta aaaaatatac tattatagtt gcaaatatgc tatgagaaaa acttctgtgc
      961 tttaaccagg gaaaaattga caacattaga caaaaaattg taaaatatat ttcaacttct
     1021 atgatgtata agcatatagc taatagtata aatattaatg atagaaaaac ttttcttaga
     1081 aatgtgtggg ttagttttgc ttcaataatt ataattgcaa caaatgtaat tattgcgctt
     1141 aaataaaaat tatcatttat atttattgaa ataaagaaaa taataatatt aacagcatat
     1201 ataaaaattc tatatatgta tttggataca ttgtttttta gaatgaaatt tgagaccata
     1261 gaattagatt tatccaaatc gggtagtaca gcaaaagtag ctgaaactaa aatatcaact
     1321 aaagatatag gaattttaaa tattaaagat gtttggatag ctgttaagat tcctattgca
     1381 caatgagttt taccacgcat aaatcctcct aaattatccg atattaaatc atataatatt
     1441 tagaggatat caaaataaaa taggtttgtc aaaataatac gttaaatgta ttttaaatat
     1501 agcttaaaat atgatacgaa ttatggtaca ataagaataa ctggtagcga gtgcgtgatt
     1561 tgatattatt tatgattgta gaacgtgtta aaaaattaaa tagacagaag gagatgatgc
     1621 aattgttcaa aaagaacacc aacaagataa aggtaatggc cttgggtgga cttaacgaag
     1681 ttggaaaaaa tatgactgtt gttgagtata aagatgaaat aattgttata gatgctggac
     1741 ttagttttcc agaggatgaa atgttaggag tagatatagt tataccagat ataacttatt
     1801 tagtaaaaaa tagggataaa ataaagggta tatttattac acatggacat gaagaccata
     1861 taggagctct tccatatata ttaaagaaaa taaatgtacc tgtatatggg gcaagactta
     1921 gtataggtct tatacaagta aaacttaaag aacataaaat gaataatgtt aagttaaatg
     1981 ttataggtcc tagacaagtt ataaagcttg ataatatgga agtagagttt ttgaaaaata
     2041 accatagtat accagatgct tactctattg caatacacac agaccaaggt ataatatacc
     2101 acacaggaga ttttaagatt gacttgactc ctattgatgg agatgttatg gatatgcata
     2161 gaatctgtga gttgagcaaa aaaggagttc ttttaatgct ggcagatagt actaatgcag
     2221 aaaaaccagg atttactatg tctgaaaaga ctgttggggt aggtcttgac gaattatttg
     2281 caaaaggaaa tggcagaaga attatagttg ctacctttgc atccaacata cacagattac
     2341 aacaaataat aaatactgct gaaaagttta atagaaaagt tgctatatca ggtcgctcaa
     2401 tggtcaatgt agttggagtt gcaaaagaat taggatacct agatatttct gatgacatgc
     2461 ttatagattt aaatgatata tgtaagtatg aagatagtga attagtgata ataacaactg
     2521 gttcacaagg tgaacctatg tcagctcttg ctagaatggc tttttcagag cacaaaaaag
     2581 tagaaataaa gagtggcgat ttagttataa tatctgctca tccaatacca ggaaatgaaa
     2641 aattaatatc aagagtaatc aatttcttat ttgaaaaagg agcagaagta gtatacagtg
     2701 atatagctga tatacacgtt tctggacatg cctgtcaaga agaattaaaa cttatacatg
     2761 cattagttag accaaagttt tttatgccag ctcatggtga atatagaatg ttaaaaagac
     2821 atgctgaaat agctgaacag cttggtatgg ataaagaaaa tatttttgtc atgcaaacag
     2881 gagatgtttt agaattagat aaaaattctg ctaaggtagc aaaccgtata caaacaggaa
     2941 acatattagt tgatggtcta ggagtaggcg atgtaggtaa tatagtttta agggatagaa
     3001 agcatttatc agaagatggt cttatgatag ttgtagttac tatttccaag gatgagggta
     3061 aggtcttagc aggtccagat ataatttcca gaggattcgt atatgtaaga gagtcagaag
     3121 atttaatgga tggagcaaag gatataatta agaatgttct aaatgaatgt gaagagaaaa
     3181 atattaaaga atgggcttat ttaaagaata atattaaaga aaacctaaaa gaatatttat
     3241 atcaaaaaac taaacgaaat ccaatgatac ttcctatcat aatggaagta taatataaaa
     3301 aaactataaa tttgaaaatt agacctcttg taatttttaa agtaatatca aaaatctaat
     3361 tgatattatc ttaagaatta caagaggtct ttttaatact cattttaaaa aatatatata
     3421 ttacataaaa taccaattat aacaatcttt tattagtaaa aactacttat atataaattt
     3481 tactgatagg aggattctta tggatgatgt ttcaagacaa aatgctataa aagctttaaa
     3541 acaaacaaaa atggaaatag caggagagta tggaatgaat tatgaagatg cttttgaaat
     3601 aatagaaaat gcgtctaata aaggtgtttt agaaggttac tttaaaaagc ttgaaaagaa
     3661 aaaaaattta ggtcaaggta tatctaggca tttagaataa aaaaagttaa atgtgagaaa
     3721 ataagtttaa ataaacttta agaggtgaca tcgatgaaaa aattagctag tactgctttg
     3781 gctatattaa ttgcgcttac tcctttaagc ttttcttttg caaataataa ggaaaatgct
     3841 gatgccaacc aactaaatat atcttcaaaa tcagcaattt tgatggatgt tggtagtgga
     3901 caaattttat atgaaaagga tgctcataaa aagcttcctc ctgctagtgt aactaaggta
     3961 atgactatgc tacttatagt agaagctttg gattcaggca agattaagct tgatgatgaa
     4021 gttcaagtaa gtgaaactgc atctagtatg ggtggtagtc aaatattcct agagccagga
     4081 gaaactcaaa aggttgatac attattaaaa ggtatagctg ttgcttctgc aaatgatgct
     4141 tgtgttgcta tggctgaaca cttagctgga agtgttgaag gatttgttga tagaatgaat
     4201 gctaaagcaa aagagttaaa tatgaatgat actcattttg caaatacaaa tggacttcca
     4261 gttgcaaacc actatacatc tgctcatgat atagctttaa tgtctagaga actattaaaa
     4321 cacgaaatga taagtaaata cttaactaca tggatggata aggttgtagt agggaagaaa
     4381 caagttacag taggtcttgc aaatactaat aaacttataa agcattatca aggagcaact
     4441 ggtgttaaaa ctggttttac tcaagaagca aaatactgtt tatcagcatc tgcaaagaga
     4501 ggtaatactc atctagttgc tgcaacttta ggagcagaga cttctccaga aagatttaat
     4561 gatgcctcta gccttttaaa ctatggattt gctaattatg aaagtgttaa gttatgtagt
     4621 aaaggtgata atatagcaac acttacattg gataaggctg atgaaaacaa agttaaattg
     4681 gttgctaaag aagatttaaa tgctcttatt aaaaaaggaa gttctaaaga atttgagaag
     4741 aaaattgaaa tagtaaaaaa tcctaagatg ccaattaaaa agggaactgt tttaggaaaa
     4801 ataaagatat gtaaagataa aaaagtaata ggcgaagtgg aattgataaa tactaaggat
     4861 ataaataagg caagctatct acaaatgctt caaagaataa ttgataatat gatataaata
     4921 attatcagat gaaatataat tataatacac aaagatatga ttataataca cataaaaaat
     4981 acccttatgt aattatttac cataggggta tttttattta ccatatagaa gtattttata
     5041 atcttatgat ttttatagaa atatagaaaa gtttatgtat cttaaaatat catttgattt
     5101 aatatcatat atagggttgt ttaacaatta tctaagtaaa ggcatactac cagtaagttt
     5161 attcactttc tttttattcc cataaattgc tatcccaaaa tactgtaact catcttctgg
     5221 aacactagca atcttttgcg taaattcttc ataaagattg caactttgtg ctacatctga
     5281 aaaatctaca acagttaaat cgttaaattc caaagtatat aattttttac gtaaatcttt
     5341 tattatttct ttgtttcctt ttaaaatagg tacaggaatg tcaataattc ctaagtggct
     5401 attacctgtt ttgtcaatta catctggccc aacagtttct ggggcatgtt ttcctagtgt
     5461 aattcccatt atagctgctg tattggatat aattcccatt ggtaaatttt cgtctattac
     5521 catgacacat tttgaatttt tattttccat ttttatttct cctttttacc ttatttaaag
     5581 ttgtttgaat catcatttat aaatatattt tgatattgct ttggtgtaag accaataaaa
     5641 ttcttaaaaa agtttgtaaa gtgactttgg tctacaaatc ctgtttgtat cgcagcatct
     5701 ataggttcta cgcctttttc taaaagtttt ttagctttgt ttacccgtat attttctagg
     5761 tatctatatg gtgtaatacc ttttaatttc gtaaaattac gaagtagaga gtacttattc
     5821 atacctgcta tagtgcttaa ttcgtcaagt actatatgtt cagcataatt attttctaga
     5881 taatcacata ctgcttgaat ttctatattt gtattttcca agttagattg caaatttggc
     5941 tctgtatgtt cttcaattaa ttgttcaatt aggaagaaaa aaagctcttc ctttttaaaa
     6001 tccaattctt cttccatgat tatatagtgt aattcttgta gtagaggtac tagctcactg
     6061 cgaaatataa caggttgatt aaattttggt aaataatttt tacctgtaat ttcaaagaca
     6121 gtttttttca ttatttctgg cttaatatta atacatctgt aatcaagtac tttatcatct
     6181 atttgttcac aagtatgact atcaaaaggg ttaaaaagta gcaaatctcc agtacttgtg
     6241 gtatactctt tatttttaca agataaatat ctttgacctt tttctataaa accaattaca
     6301 taatgttcat gaaaatggtt tgggaatttt tgcattatgc ctttaaatgt atatgcttca
     6361 atttttaatt ccctatcaaa gcatactgtg cgttcttctt gtctcactta ggtaacctcc
     6421 tttcatgtta tacaaagtgt aacacatatt tttttatact tcttgtataa tattgactaa
     6481 gagacaataa tgtcaattca tgtcaatata atattacact tacagtaggt gtaattatta
     6541 tattt
//
LOCUS       DAMJYC010000162         6532 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJYC010000162 DAMJYC010000000
VERSION     DAMJYC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.169"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(2..715)
                     /locus_tag="PC472_003757"
     CDS             complement(2..715)
                     /locus_tag="PC472_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2729032.1"
                     /translation="MGKVAKILLSLIVIVLVLTGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1007..2671
                     /locus_tag="PC472_003758"
     CDS             1007..2671
                     /locus_tag="PC472_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2729033.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2778..3677)
                     /locus_tag="PC472_003759"
     CDS             complement(2778..3677)
                     /locus_tag="PC472_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2729034.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3704..4342)
                     /locus_tag="PC472_003760"
     CDS             complement(3704..4342)
                     /locus_tag="PC472_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2729035.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4679..5677)
                     /locus_tag="PC472_003761"
     CDS             complement(4679..5677)
                     /locus_tag="PC472_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2729036.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFITSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5775..6350)
                     /locus_tag="PC472_003762"
     CDS             complement(5775..6350)
                     /locus_tag="PC472_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729037.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2211 a         1037 c          726 g         2558 t
ORIGIN      
        1 tttaaccaac agatgttttc ttcaaattaa tcagatgatt tagtatatct tgaacctttc
       61 cagagtcctt taaatatcct aatgctccat ttatttgtga ttttaaatct atttctactt
      121 ccatatccaa atcaacattt ccttttttta tagatatatt tttaagtgtt attgggtaaa
      181 gataactttt ttccaaagta atcttatttc cattaacctc aaatggaact tttttattgt
      241 agttacttaa tattttttct agcattttat cacttagttt aaattttcca attttcacat
      301 tctcaagact tacaactaag tttccatcct ttaaagtagg tctcaattca agttcatatt
      361 gactatttac caaaccaaat accttatatg gtcctgctac ctttatttta ccatttttca
      421 tttcaacaaa attattttca agttcattaa ttccatgctt ctgcatcaat gtatatatta
      481 aatttttaaa ctcttcctct gaaacagata tttttccaac cattctaagt ggatttttta
      541 ctacctccat agagtttata tatgagcctt ttgataaata ttcttcatct atgattttat
      601 tattttgtga actaacatca tatctttctt ttggtgtcaa gatatataca acaattcctg
      661 taagtactaa aacaataaca attaaactta acaaaatctt tgcaaccttt cccataatcg
      721 tctcctttac aaatctgtca tcagatttaa aataaattta gataactaac ttcacataat
      781 attcttaaca atatacatac tataatacag tcttttaaaa attaattcaa ccaatctaat
      841 gttttgtaaa taaactgtaa aaacatgttt ttagtataaa agttaatatt ttgcataata
      901 ctcttaatta cacctttatt tttttctaat cttggattaa aattaatatt atgcaataca
      961 gtgtagtgtt ctgtattata atcaaattaa ataggaggta tagaatatgt caaatgaaac
     1021 gaactcatct aactttataa agaatattat tataaatgac cttgaaacag gaaaacatga
     1081 cagtatcata actcgttttc cacctgagcc aaacggatat ttacatattg gtcatgcaaa
     1141 aagtatatgt cttaattttg gattagctaa ggagtttaat ggaaaagcta atttaagatt
     1201 tgatgataca aacccattaa aagaggatgt tgaatatgta gagtccataa aagaagatgt
     1261 aaagtggtta ggatttgatt ggaacgagtt aaattttgca tctaactact ttgatgaaat
     1321 gtataagaga gctttaatac taataaaaaa gggtaaagct tatgtatgtg atttgacaca
     1381 agaggaaatg agagaatatc gtggtactct aactgaacca ggaaaagaaa gtcctcatag
     1441 aaacagaact attgaagaaa accttgattt atttgaaaga atgaaaaatg gtgaatttaa
     1501 agatggtgaa aaaacattaa gagccaaaat agatatgtct tctccaaata taaaccttag
     1561 agacccaatt atatatagaa tatctcattc aacacaccat aatacaggag ataaatggtg
     1621 catatatcct atgtatgctt ttgctcatcc aattgaagat gcaattgaag gaataactca
     1681 ctctatatgt actttagagt ttgaagacca aagacctctt tatgattggt ttgttaaaga
     1741 atgtgagatg gaaaatatac ctagacaaat agaatttgct agacttaata taaataatac
     1801 tgtcatgagt aaaaggaaat taaaacaact tgtagatgag ggtattgttg atggttggga
     1861 tgaccctcgt gttcctacaa tatccggaat cagacgtaag ggatatactg cagaagcatt
     1921 gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac tctacggtag atagtcaaat
     1981 gcttgattat ttcctaagag aaaacttaca accaaaggct cctcttacta tgggagtttt
     2041 aagaccttta aaacttatta taactaatta cccagaagat aagattgaga tgcttgaaat
     2101 agagaataat gctaaagatg aatctcaagg aaagagatta gttcctttct ctagagaatt
     2161 gtatatagaa caagatgatt ttatggaaga acctgttaag aaatatttta gatttttccc
     2221 aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg taattaagga
     2281 tgaaaatgga aatgtagttg aaatacatgg tacttatgac cctgaaacta agagtggctc
     2341 tggctttaca ggacgtaagg taaaatctac aatacattgg gttgatgcta aatcagctat
     2401 accatgtgaa tttagattat ttgaaccatt aattcttgat gatatacctg aaaatgaagg
     2461 taaacatttc ttagaacaaa taaatcctaa ttcattggaa atattacaag gttttgttga
     2521 acctacacag ataaaagatg ctaaaccttt tgataaattc cagtttgtta gaaatggatt
     2581 ctttagtata gataataaat atacaactga tgaaaaattt gtatttaata gaatagttcc
     2641 tctaaaaagt tcttttaaac caggtaaata atcttacaat caaaaaatga agacttaaaa
     2701 tataaattac ttctactata aataaactta gttagaagta attttttatt tgagtcttca
     2761 tcatttactt taaatgatta acttttttca ctacagattc ccatgacttc tattccatct
     2821 tttatgcatt ctatgttaag aggaaaatct ggtccttttt ctccgtcaat atctgttata
     2881 agtgaatctt cacattctat atgcaccttt ttagttttaa agtatagtat ttcatttcca
     2941 ttatattgat ctaaataatc acctctaagc acacttatca aaactggtat tgattttggt
     3001 attggcattc ctttaaaaat aattacatcc aaatatccat catctatttc agccttatat
     3061 gctagattta tatttcctgc tgttttgcca ttaaaaatca acataagata catatctcca
     3121 tcataataca tttcctcaga agatactttt atcttaaatt tcctcatata caatgcttct
     3181 tctattccct ttatataata agaaattctt ccaaatgaat tttttaagtc tgtgtttatc
     3241 ctttgtgata catccgtaaa cataccagca cttgcaacat ttataaagta cttattattg
     3301 actttcccta tgtctatttt ctttggtttt gaatttatga tttcatttat agcttctttt
     3361 atatcaaatg aaatttgaag agcatttgaa aaatcatttg cagttcctgt aggcaaaatt
     3421 ccaattggaa tttttatgtc taattctttc attgcattta ctactaaatc tacagtacca
     3481 tcaccaccag ctatcaatat gtgttcataa gaactatcaa cttccttaaa tgcattcttt
     3541 acaggttctt taatagttaa tctatagggt atcaataaat aatttttttc ttgatataat
     3601 tctatgatag tgtctaaatt tgataatatc tttttttcac ctgaattagg attgtatata
     3661 agttttactt ttttcattat ttctccttac tcattttatt cttttagtat ccaaaatctt
     3721 catttaaaaa aatcacatgt attctttctg gtgttaattg tctttttata agtgtataac
     3781 tacagcatat tcttcctgga cttgaacagt ctatacatat accagtagtc ttacatccag
     3841 tatttgtact taatctttta caattggctg gagcagctat tgtttttgtt ctttttatag
     3901 cttcatctac atctttaact attttattta ctcctgctat aactattact ttatcaggtc
     3961 catatatcat agcagcaacc ctattcccat tcccatctaa attgtaaagt tctccatcct
     4021 ctgttatcgc attagtactt gtaaaataag catctgcaga aaatgacttt ctatatattt
     4081 ctttcatttc ttctggtttg atatcttttg catatctatc aagaaaatta tatttttctc
     4141 ttataaaatc aatcactcct gtttcaaata aggtcataga acctccacat cctactagag
     4201 aacctgcttt aactatttct tcaatttttt taagaagctc ttctttgtta cttactaaat
     4261 accctttcat attattgttt tccaaagatt ttattgtttt ttctattctt ttttcattaa
     4321 gccaatttaa attttgatcc attttaactt cccccttatt tttaatatgt acttaattat
     4381 ttataaactt tattttcttt ataagtttat tttagtacat ctaaaaaaaa atacaatata
     4441 aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa aaaaggaaag
     4501 tagatgtact attctacttt ccctatattg tatgtagtat ttattgccta aaatctatat
     4561 attaattata tgtttcatat tatataatat cacagttaat taaaatgtaa tactatcttc
     4621 tttttaaaca tataaattgt attattaaat ttaatatatc caacttcaat aaatcttatt
     4681 aatttgatga agaagatgct atagctgcac ttgttgctgc cattacacat gcaatttgaa
     4741 tagcaacaac aatatttaaa aatatattat tagttataga gcctatatta ttctcatttc
     4801 gtatcaaatc tgcataaatt gaagctacaa tagcagaact aatcattact ctattacttt
     4861 ttcctaaaga ccaatttcca aagccttttt cttctttaag tttatttgaa acatttttta
     4921 tctcttctat tatttcatct tctctataat ctaataatga aattgctcct attattggat
     4981 atccataata attcatttta caatcatttt ctttaaatgc ttttttaagt tcaatagtct
     5041 ttttacattt aagcatagtt ctatcatcac taaataaaag aatttgggat aatgcttgta
     5101 aatcatttcc tttataaaaa ccattttcat ttagaaattc ataacatttt tctatgtatt
     5161 ctaaatcttc atctaaattt tttgaatgaa ttgcaataag tgctatatta caatagtcat
     5221 cactagaagt tataaatgga tgttttttcc tcatgtaatc atatattatt ttcatttttg
     5281 atatacatgt aagataatcc attttctctc tattttcata aactattgat gctgtaagtg
     5341 gtaaatatgt atcgccccaa aacttttcac ttttaagttc tttgtatata tctaaaatca
     5401 tcttaaaact actttctgga tttgattcaa acgaaagtag tgttgccata taaaacatat
     5461 tatatcctct aaaattagag aataatccag tatttttttt aataagctct attcgttctt
     5521 ccactaatct aggacttatt ctcttgttct taagggtaaa tgcaagtgca caactatgtt
     5581 gtatcaatcc catttgccaa gaacctttaa cttcatttaa tgctttgtaa ttttctatta
     5641 ataagtttac ttttccattt ataaacgatt gttccatatt gccctcctag taatatatct
     5701 cattgtacta atagttttat atttattata tatatattaa atactacaat caatacatat
     5761 tacttcaaag aacatcattc ttcaacaata atgtagcctt ttttgtaatg tcttcccata
     5821 ttattgactc tatcatagta ctctgattca ctttcattac tccatttggg cacaaataca
     5881 tctattgcat tagaattact aaaatactta ctagaccccc tatccataac agtttttaat
     5941 tttccatcta caataatctt agttccaaaa cttaagtgat tgtttgctac aaaaccatct
     6001 tctaaatcat ctcctgtagc agttatcgct ccatatccat tttcttgatt aagacttgtg
     6061 taataactaa cttcaaaagt tataggtact aacttcttta ttttaatacc taactgattt
     6121 tgtagtttct ccttttgtac tttcaactta tactcatcac tatttttata atcttccata
     6181 ccttgattat acttggaatt aaagctttta cctgacttta ctatagtatc tatatttact
     6241 atagattctt tacaatgatc tgcatctatt tttgtttctc catttaccaa taatggagtt
     6301 aaacatacac tacatacaac acatgtaatc aatgcttctt taagcttcat aaattccccc
     6361 cttattattt tgatatatta gcatatttaa tattatatgt ctacatttag atttaaatac
     6421 cacacccatt tgctttaaga agttgtctta tatttaattc accttaaaca cttataaatc
     6481 ctctatctta gaatcatata gaataaagct tttatcttta aatatttttt at
//
LOCUS       DAMJYC010000163         6436 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJYC010000163 DAMJYC010000000
VERSION     DAMJYC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.170"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..967
                     /locus_tag="PC472_003763"
     CDS             <1..967
                     /locus_tag="PC472_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2729038.1"
                     /translation="LNISKVNMFFDGGKFIGGKSIVFSIIGIIFLIILVSGIAYGYWV
                     TKCISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHRSDEIQNKTDIMREE
                     WISNITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKNIEYTNTLINDLKL
                     TYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSNIDNINFDFDCTLL
                     KRAFSNLIVNAFTYGDKNTKVCVHITLNERIEIIISDNGKGMSREEASNLFNRYYRGS
                     NTKQKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI"
     gene            1186..3663
                     /locus_tag="PC472_003764"
     CDS             1186..3663
                     /locus_tag="PC472_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2729039.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRIYPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIETFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIIGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3678..4364
                     /locus_tag="PC472_003765"
     CDS             3678..4364
                     /locus_tag="PC472_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729040.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4638..5471
                     /locus_tag="PC472_003766"
     CDS             4638..5471
                     /locus_tag="PC472_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729041.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5509..5964
                     /locus_tag="PC472_003767"
     CDS             5509..5964
                     /locus_tag="PC472_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2729042.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFIIQFQ"
     gene            6001..6240
                     /locus_tag="PC472_003768"
                     /pseudo
     CDS             6001..6240
                     /locus_tag="PC472_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
BASE COUNT         2479 a          689 c         1006 g         2262 t
ORIGIN      
        1 attaaatata tctaaagtga atatgttttt tgatggaggt aaatttatag gtggtaagtc
       61 aattgtattt tcaatcatag ggattatatt tttaataatt ttggtttctg gaattgctta
      121 tggttactgg gtaacaaagt gcatatcaaa tattacatct gctgttagcg atatttcaaa
      181 acgaagctat cttccagtta atattaaagg tgtttttgct gatatatata atagtttaaa
      241 tattttaaat gaagaaattc atagaagtga tgagatacaa aataaaactg atattatgag
      301 agaagagtgg atttcaaata taactcatga tttaaaaact ccactttcac caataaaagg
      361 gtatgcagag cttcttacag atagtagtac taattattca cctgaaaaag tatataaata
      421 tgcttcaatc atgttaaaga atatagaata tacaaataca cttataaacg atttaaaatt
      481 aacctatcaa ttaaaaaatg gaataatccc tttaaataaa aataattatg atttagtaag
      541 atttttgaaa gaattatcca ttgatatttt aaactatcca gaatatgaat caaatatagt
      601 tgaatttaag agtaatatag ataatattaa ctttgatttt gattgtacac tattaaaacg
      661 agcttttagt aatcttattg tcaatgcttt tacatatgga gataaaaata ctaaagtttg
      721 tgtacatata acattaaatg aaagaattga aatcattata tctgataatg gaaaagggat
      781 gtcaagagaa gaagcatcaa atttgtttaa tcgttattat agaggaagca atacaaaaca
      841 aaaaatagaa ggaactggtt taggactagc aataacgaag caaattattg aagttcatgg
      901 agggaatata tctgttgaaa gtatacctgg attaggtagc tcattttata tcacctttcc
      961 tatttaatta aggttaatta aggtcaaata aaaaatatat taaggttgat agattattat
     1021 atttatatag tagtttatca atctttttta tttaaggggt gatttgatta aaaatattat
     1081 aaatagaatg ataaaagtat ggaggagtct attagagatt taatttagat taaagtataa
     1141 aaatattaga ttgaaatatc ttagataagg aaagaaggtg taaatttgaa aattatatta
     1201 aagtatattt tcacaaatat taaagaacga aaaattagaa cagcagttat gttattgtcc
     1261 atagttttat caacagtttt actttttgtt tcattctcaa ttggattatc ttatgaaagt
     1321 gcacaaagaa aaatggcaaa aggtatgtat ggtacagcga ctatatctgt ccaaagtaaa
     1381 aatccagata tattaaccaa tttagaagac atacctgatt taaatactat aaaatcaaag
     1441 gttggtgtat tggaaagctc tgctatttat aataaagggg gatactatga agaatttagt
     1501 cttatatcag cagacttaag tcaactaaat aaaatcaaca agccacgatt agaaaatggc
     1561 gatagtatta ctgatttttc tggtgataaa attattcttc caaatcgctt tacatcaaaa
     1621 tataaaataa aaaaaggtga ttctattact ttacagattt atggaaagtc ctatactttt
     1681 caagttagtg atattgcatc ttatgataca gtatttttga ggaacacaag aggtgtaaat
     1741 gcactgctac ccaaagagac tctatcaaaa attataaata aaggcagtgg atatacaaga
     1801 gttcttatag agtcagaaga agaaattaca gaaaatctag taaataaatt atccgaagaa
     1861 ctttctactg aaaaatatat ggtatcaaat actataaatg aaactaagat aatttcagat
     1921 gctagacaaa agacaatgcc tttcttttta ataagttttt ttgcattaac ccttagtata
     1981 tttataatat acagcagtta taaggttatt acattagaac gtcttccttt tattggaaca
     2041 tttagaagta ttggtgccaa tgaaaaaact gtaactcgta tattgatgct tgaaagtata
     2101 ttatacggaa gtataggagg attaattgct attcctattg gtgtagttgt tttaaattta
     2161 atgcttcatg ggttaggaaa ttctctcgaa caaggaatat caattccagt agtaatatca
     2221 cccattggag tgattatatc tgttatagta gcaataatcg tttcttcatt tagtgcatat
     2281 atacctgtta aaaaagctag ccatttgcca ataaaaaata ttgtactagg aacagtagaa
     2341 gaaaagaatg tttcaaatcg ttctatactt tttataggaa gtataatgtt tattttatct
     2401 attttacttc caagaatata ccctgaaaat acattgtatc tagctggtgg attttcatta
     2461 ctaggattaa ttgtagctac tattgtgttg attcctctta ttactgatat catgtctata
     2521 gtttttgagt ttgtttataa aaatatttta gggaatgagg gtaaattagc tgctagaaat
     2581 atgaaaaata acaaaaatat aatacagaat atcactttac tatttataag tatttctgca
     2641 gttattgcaa taagtgttgt tggtaacttt gtcaaaactt atataacaga tgtgtttcgt
     2701 gacgcagaat tacaaggttt tgcagatgga aagatgaatg aagagtttat tgaagatgtt
     2761 agacatatgg atggaattaa aaaaatatta cctctttatg ttatgaataa tgagatttct
     2821 ggtaatggtg taacattatc aagactagag ggtacagata atataaaact atataactct
     2881 atgtttggaa tcaattatac aaattttgag ataaaaaaac aagtaataga aacttttaat
     2941 gataaacgtt ctgttatttt gaatgaagat actctaaaaa aagtaggctt atctatagga
     3001 gatacaataa ctctatctaa tgataagtat gatttttctt ataaaataat aggtagtttt
     3061 aaatcccgtg caaatgatgt agaagctgtt ataccatcac attatgcagt taatgatttt
     3121 gataaaacta attatggttt tttagtatat acagcagtca atccagatgc tgttatgatt
     3181 cagataagat atttatttgg agatacatat aattggagtc gtacagttga agaatttaat
     3241 aatgattctt taaatactat aagtagtttt ttgtctccta tgaataagat gacctatttc
     3301 atttttcttc ttgctacagt aggtattatt aataatctac tgattaatta tattcaaaaa
     3361 agacgttcta tagctatgta taaatctata ggactaagta ataaacaaaa tataaaagtg
     3421 acattgattg aagggtttac ttcaggttta cttggagctg ttattggtat tgtgatatct
     3481 atacttgaga ttcaaactat ctttatagtt gcaggaccaa aaatttcaat gaaaccagat
     3541 ttagatttta aaacatttat tattgttggg ttattgggaa ttatagtaac tttaataggt
     3601 tcgatagtgc ctattataaa agggaagaaa atgaaactga tagaagaaat taagtttgaa
     3661 taattatgga ggttaatatg aaaattacac ataatagttt agctgtagaa gcaaaaaata
     3721 ttatcaaaga atataaaatt ggaaatacaa caactcgagt gttaaaagag gtatctttac
     3781 aagtaatgaa aggtgaattt gtttctatca tgggtcaatc tggttctggt aaaagtactc
     3841 ttttatatat tttaggtggt ttagatactc caacgagcgg aaaagtgtat atgaatggag
     3901 ctgatatatc acattttaat gatgagaaaa tgagcataat acgtcgtaga aatataggat
     3961 tcgtatttca attttataac ttaataccaa atttaaatgt ggaggaaaat ataatgcttc
     4021 ctttgttatt agatggtaaa aatttaaaag attataaaaa tcaattagat gaaatcttag
     4081 atatagttgg tttaactgat agacgtaaac atacaccacg agagttatct ggtggtcagc
     4141 aacaacgtgt tgctatagct cgagctttaa ttggaaaacc agagatttta tttgctgatg
     4201 agcctactgg aaatttagat agtaaaacag ggatagaaat aatagattta ctgaataaaa
     4261 taaacagaga caatggacaa actataatta tggtaactca ctcaccagaa gcagccaaaa
     4321 gtagtagtcg tactattaca gtttcagatg gtttaattgt ataaggacta attattgtat
     4381 ttgagataag catttttaac tatataaatt agacatatgt aaagtggata taagttaaat
     4441 actataaatg tgctataata ttaaaataac agataaaagt actatatttt ttattgatta
     4501 ttttaatttt atttgtttat taaatatcta atgagtattc atactatctc tattattaaa
     4561 agcagttaat gatatataga gataatatgg attcttctta ctttaataaa tattcacgat
     4621 aggacaggat ataatatatg ttaaaaaaag aaagtattca ttttataaat aaaaataggt
     4681 cacttatagt ttgtgaagac aattttgttt atctcttgcc ttgtatacaa ttaagaaatt
     4741 ggatatcgaa ttttactata tcgtttccaa atcaaacaat aatttctgat aattacacga
     4801 ttattcctca tggaagtgta actttggtgt ttttttatga tgccacaggg ttacatagtc
     4861 tattatttgg tccaactact aaaccaaaaa cagtaggaaa tattgctaat agatgtgatg
     4921 ttatactaat tatagaattt caacctgctg gttttttccc gcttataggt atacagcaaa
     4981 gtgaacttat tgataaagtt gttccatttt ctataattaa tacttcatta gatttagaaa
     5041 ttaaaaaaat atttaatgaa tctttatcta tagataaatt gattttaaag ttggaagaat
     5101 tattgatttc taatattaaa attgaatact catatgagtt tattttggca attcagttga
     5161 ttattcaaaa ctctggaaac atatcttcac aagaaatttc taaaaaagta ttttatagtt
     5221 ctaggcattt aaacagactc tttaatcagt acctagggtt gagtatgaag tcattttctc
     5281 gtttggtacg tattaataaa tctataaaac ttctaaacaa taataaaact tcattgatgt
     5341 ctatatgtaa tgaattagga ttctatgata taccacattt tattaaagat tttaaaattg
     5401 tctgtggaat aactcctcaa aaataccgag ctaatatgtc tgatttttac agtgaaattg
     5461 caaaatttta agttataata cttataacaa agaaaggata gtgataagat gaaatttgga
     5521 ggagtattgc taattgtcaa agacataaat aaatcaaaaa agttttttga agatgttatg
     5581 gagcaaaaaa tcacattaga tttaggagaa catgtatcat ttgaaaatgg atttggatta
     5641 caaagtaatt ataaagctat aattggatat gatttaccaa tatatcaaca atcaaataac
     5701 tttcagttgt attttgaggt taatgacatc aaacaatggg aaagtaagat aaatagaata
     5761 ggaaatattg aatttttgca ttatataaaa gaatacccat ggggtcaaag aacttttagg
     5821 ttttatgatt ttgataaaaa tattatagaa atttcagaaa gtatggaaag cgtcataaag
     5881 aggcttttaa aacaaggttt agctttagaa gaaatttcaa aacgcactat gtatccagtt
     5941 gaatttatta tacaatttca ataattataa aatattaaaa tatataattt tttctttata
     6001 ttgcaaaaaa caaaggcttg gggcataata gaaccagaaa aacctgaatc tcaacagcaa
     6061 atggctcata tggatttcgc tgttaatgat ttagaagaag cagttcaata tgcaattcat
     6121 tgtggagcta caatcgcaga ggagcaattt actgatgact ggagagttat gattgaccca
     6181 gctggacacc atttttgctt atgtcagatg aaatcaatta tggaaagtag ccatttttca
     6241 ttttatagac caaaatattg tttattaatt acaaaaaagc acaacagtat ataccattgt
     6301 gcttttactt attatacatg aatacactaa aaaatataaa tagtagagct ttatcaataa
     6361 tgataatttt taaaaaatag tattttttta ttgaataatt tcttagatat tttaaagtat
     6421 ataaattttg catata
//
LOCUS       DAMJYC010000164         6435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJYC010000164 DAMJYC010000000
VERSION     DAMJYC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.171"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            157..3336
                     /locus_tag="PC472_003769"
     CDS             157..3336
                     /locus_tag="PC472_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2729043.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3517..3600)
                     /locus_tag="PC472_003770"
                     /pseudo
     CDS             complement(3517..3600)
                     /locus_tag="PC472_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3621..4385)
                     /locus_tag="PC472_003771"
     CDS             complement(3621..4385)
                     /locus_tag="PC472_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2729044.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4541..4774)
                     /locus_tag="PC472_003772"
     CDS             complement(4541..4774)
                     /locus_tag="PC472_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729045.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4848..6341)
                     /locus_tag="PC472_003773"
     CDS             complement(4848..6341)
                     /locus_tag="PC472_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2729046.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2390 a          911 c          743 g         2391 t
ORIGIN      
        1 agataattca ataaaaatct aatagtaaat tgttatttac acatatatcg tgatagataa
       61 aagctattca tatctgcctt aattttagtc ttaaatttag agtgtaaatt gaatgcattt
      121 agattataat atatacagtg aggtaaggtg aataatatga aaatagatat gatatataaa
      181 ctaataagaa gaaatacaga accatcaaga tggtcaagag gtgtaaaata ttatactaat
      241 caattagttg attatataga catagatatt gatgacctac aaaataatct gttgacattt
      301 cgtggtggag tcaattctga agataattat agcttttatt ttaattcaat aactatagat
      361 ataaagcact ttaagataat tgaaaatgat tgtagttgcg ctgactgtca atcaaagggc
      421 aaaagtattt ttatatgtaa gcatatagtt gctattgtga taaaaagctt aaaacaaata
      481 aaaaataaaa aagatgttcc tcctattcta atagatatat taggtgcaga taaggataag
      541 ctttctaata caaattctaa aaccagctta gatttaagca agcaattact ttctttgtta
      601 gacaatacta aaagagaaag ggttaattta gatgtattga taaatgtgga ttcaaataaa
      661 gcaattgctg aatttaaaat aggaattaat aaaatgtaca ttttaaaaaa cattactgaa
      721 tttgcatatt caaaacttaa tggatatgaa ttagattttg ggaagaaatt tacttacaac
      781 cccaacactc attattttgc tgatgaagat gagaagttag tagatattat agaagaatat
      841 gggacaaatc tttcatacaa cttttcttca tcacattcaa aaaaatttat ggagataaat
      901 gcaagctctt taaaacaatt tttaaatgct attaagtaca aggattttac ccttattttt
      961 tctaaagagg aatatcatcc aaagatatta aatgatactc tacctgtaag gtttgatttt
     1021 aaaaatatag aagataaaat agtcttatat tctaaagatt cactacctgt tccacttact
     1081 aaaaaaaatg atgtactttt atatgatggc aatatctatt tattatcaca taaaaaagct
     1141 catgattatt caaaaatata tcaaatatta tcaaagacta aagagttaaa atttgataaa
     1201 gaggattcta aggatgtttt aggattgcta gttcctagat taaaatctat atctcaagaa
     1261 gttcatttag atgataacat aaaaaataat ataacaaaag attttaaatc tgagttctac
     1321 tttgatatga ttgatggcaa catatgttgt gatgtaaagt atatatatga tgatgaagat
     1381 aaaaaatttg tgctccccaa catacaaaag gaagctacta ttgaaaataa acttacttct
     1441 tcacaattta caaaggaaaa taatcattat gtgtttaaag gaactgacca tgacctattt
     1501 acatttttag atttacaatt agaatctcta aaggaatttg gagatatata ttattctgag
     1561 aagtttaaat tgaaaaaaat atataacgct tcatcaattc aagcttcaat caatcagact
     1621 aaccagaatt acctagaatt taattttaat atatctgata taaatccaaa agagtacaag
     1681 gatattttga aagcttttca agaaaaacgt actttttata aacttaaaga tggaaacttt
     1741 atagatttat cagaaagaga aacaaaagat ttttttgaat tagttgaaaa tctagatata
     1801 atggataaat caaaaaataa taaaattcat aaaaataaag ctctatatat aaatgatgta
     1861 atacgtagta aaaatttaaa gtttatagat ggtaaaaaag aactagacag tatatgtgaa
     1921 aaatttagaa attttgatag cataaattta gaaattcccg caaatttaaa tgcaaacctt
     1981 cgtgactatc aaataaatgg cctcaattgg tttaaagttt tagattatta taaatttggt
     2041 ggtatattag ctgatgaaat gggtcttggt aaaacaatac agactattgc atttttatta
     2101 tcattatcta ataaaaaaag tcttattgtt acaccgacat cactcattta taattggaaa
     2161 aatgaatttg aaaagtttgc tccagatata aaagttttat tgatacatgg aaataaaaga
     2221 gatagagaaa aatgttttat ggaattagaa aattttgatg taatacttac aacatatggt
     2281 actcttagaa atgatttaga aaaatatagt gaaataaagt ttgattattg tatattagac
     2341 gaagctcaaa atataaaaaa tccagtagct ttagtaactg agtctgtaaa aagtataaat
     2401 gcagaaaata aatttgcact cactggtaca cctatggaaa ataatctact agaattatgg
     2461 tctatatttg attttataat gcctggctat ctatatagta aagcaaagtt tcaggaattg
     2521 tttataaata aagaagataa tgttaaaaac ctgaaaaaat tgataaaacc ttttattctt
     2581 agaagaagta aaaaacaagt tatgaaagaa cttccagata agattgaaaa aaactttttt
     2641 gttgaattga ataaagagca gaaaaagata tatagtgtat attcaaaaga tatacaagat
     2701 aaaatgaaag ataaaaatct aaagaaagat aaaatagtta tattttctta tttaactaaa
     2761 ctaagacaac tttgtttaga cccaagtata gtagtaaaag attacaataa aaaaagttca
     2821 aaaattgaaa catgtttaga aatacttagg gatagtataa acgaaaacca caaaatatta
     2881 ttgttttcac aatttacttc tgtgcttaag aacataagta aggaacttga taagtataaa
     2941 attaaatacc attatataga tggtaaaact aatgccaaag aaagacttga acttgtagat
     3001 gagttcaata atagtatgga taaaaaagtt tttttaatat ctttaaaagc aggcggaact
     3061 ggtttaaatc ttacatctgc tgacatggta attcactttg acccatggtg gaatccatca
     3121 gtagaaaatc aagctagtga tagagctcat agatttggtc aaaaaaattc agtacaagtt
     3181 ataaaattga ttgctaaagg gactatagaa gaaaaaataa taaaattaca agaaagtaaa
     3241 aaagagctta taaatcaatt tataaatgga gaactttcta atgaaggtgt attaaaaagt
     3301 ctatctgatg aggagataat aaacttattt aactaaatta tttaatccta cagcttaact
     3361 atatgaattt gttctaacat ttatatagtt aagctaaaat atctgaataa ataaaattta
     3421 atattgttat ttaatcccaa ctttacaatc taatgatatc tctttctata catattgcat
     3481 atacaaatgt aataacttga ctattagaat attttgtttt aacaatccta tttcatcata
     3541 ataatgaagt atatgtacac tcacttttgt aagttttgca atccatttta cagttttcat
     3601 tattgtaaat tttggttaaa ttattcctct aaaaatttct ttgctacatt tgaaaaatct
     3661 aatccattcg ataaaattgc aggatgacca cctttatcaa acaaatacat tttaccattt
     3721 ttaatctttt caagaagtaa tgaaaaagta ttttcaaaaa aatcactaga tattagagat
     3781 acaaactcat cttctctact tcctgttaaa agcacctctg gttgcatagt ttctagaggc
     3841 ttatgaaaaa actttcctat agttttataa tgttcaaaaa tagcttctgt atcattatct
     3901 acaacttttt cccagttttc tccttgatta taaatataaa aagcttttgc accatcatct
     3961 tgcttagatg cttcacgctc tattttcaca ttttgtacaa atgactctag aggaacttct
     4021 ccttcaaaac tatctgcaat taccttattc actaaatcag gtctctcaag tgcaacattt
     4081 aatgcaacaa gagctcctcc actactacca attatgttta cttttttata gttcattgct
     4141 tctaaaaaac atatcacttg catagcctca tcaaaccata agtcagcaga aaatttatcg
     4201 accatttgag attttccatg acctacaaaa tcaattaata ttactttata ttcatcttta
     4261 taaaaattga ttatatcatt aaacatcttg gaagaggctg tatttccatg taaaaaaagt
     4321 aatggtgtac catccccaat ttcattatag tagcactgtt tgtcgttata gttaaaatat
     4381 gccatatatt tccccccttt aatatcccta taggtatata tttattactt atatttaaaa
     4441 tgactattcc cttagccact taaaaatagt ataacttaga atgtagataa aatcaacatt
     4501 ccttgctaat ttatctattg tttgtctaat ttattataat ttaacttctg taaaatttct
     4561 tttttgtacg aaaatattcc ttccatttac aacaatcatt caaatgaagt ataacatgac
     4621 gagtaggagg cataagcaaa attccagcaa tatctttatt cccccaaaag tccaacaacc
     4681 aaatagaatc ttcatgcttt attactcctc cagtcaccaa aatcctttct aagtcaattg
     4741 gagagacctt tcgtttcata tcttcagctt tcaaatcctt aacaacttct attgtgtttt
     4801 ttgcaacttc atttcgataa caaagcaatt catcaatatt gactaaatta tcgtatatct
     4861 cttttcactt taaccatttt tttctttata ggtatctcct tatatttttt taatatcaaa
     4921 tctcccttat aatttaatat atctacatat gaacataaat cttgaacttc aattacacct
     4981 atatcatcat tagttatacc ttttatatta ctaaatgcac caactatatc taaaactctt
     5041 attttcttct tcttaccagc atttaaatat aatttaacaa cttcactttc tatatttagt
     5101 tttatatctt tacttttgta agaattttta tcaacttttt ttctctttga tacttttttt
     5161 aggattcctt ttgaatattc ttcaaacaaa actttgccat gtgtaattct acttttttct
     5221 atatctttta attcatttat tttatatcct atatacgttt ctatattctc tatatatttt
     5281 tcatcctttt cagttactat agtaattgcc tttccactat ttccttttct cccagttcta
     5341 cctattctat gtatatagtt ttctttatct tgtggcacat cataatttat tactaaagaa
     5401 atatcatcaa tatgtattcc tctggacgct acatcactac taactaatat attaaatttt
     5461 tggtttttaa aatccttaat tacaaaaatt cttctttctt gtgataaatc tgcatgtaac
     5521 tctcttatta aaaaaccctc tttactcatc ttgttatata atttagatac tttctctttg
     5581 gtattacaaa atattatcac actctttgga ttttcgctgt atattaattc ttttaaaagg
     5641 atgtacttct ctcttccatt tgccttgatt attttgtcat ctatttgtct tgttttttga
     5701 ttagtatcgc tctcattttc ttctatattt ataacactgt aatctagcat atatttttca
     5761 catatatatt ttatctcttc atctattgta gctgaaaaaa gacttactat tttctcctta
     5821 ggtaaattta ataatatttt ttccatttgt tcaacaaaac ctttattaaa cattttatct
     5881 gcctcatcta ttactaaata ctttacattt tccaacttaa tagaacctct atttatatgg
     5941 tcaagtattc tgcctggagt agctacaaca atatgaaccc tttgtttcaa ttcagctatt
     6001 tggtctttta tagattgttt gccaaatata gcactacatc tcaccttctt tagtctccct
     6061 atatctgata tctcatcttt tacttgcaag gctaattctc ttgttggaac cactataaga
     6121 gcttgaatat tattgtaatc tacatttata ttctcgcata aaggtattgc aaaacttgct
     6181 gtctttccac ttcctgtttt tgacctaact actaagtttt gaccttttaa taattttgga
     6241 ataacttctc tttgtactct agaaggtata ttatatccta aactcttcaa tgacttaagt
     6301 atcttttcat ttaacttaaa tttttcaaat gtatatttca tatattacca ccttattata
     6361 tttatcttat ctatatattt ttctacatta tattattata atacaaggtt ctactatttt
     6421 tatattattt attag
//
LOCUS       DAMJYC010000165         6313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJYC010000165 DAMJYC010000000
VERSION     DAMJYC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.172"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            238..1182
                     /locus_tag="PC472_003774"
     CDS             238..1182
                     /locus_tag="PC472_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2729047.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1204..1830
                     /locus_tag="PC472_003775"
     CDS             1204..1830
                     /locus_tag="PC472_003775"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2729048.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1864..2913
                     /gene="hisC"
                     /locus_tag="PC472_003776"
     CDS             1864..2913
                     /gene="hisC"
                     /locus_tag="PC472_003776"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2729049.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2916..3494
                     /gene="hisB"
                     /locus_tag="PC472_003777"
     CDS             2916..3494
                     /gene="hisB"
                     /locus_tag="PC472_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2729050.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3496..4101
                     /gene="hisH"
                     /locus_tag="PC472_003778"
     CDS             3496..4101
                     /gene="hisH"
                     /locus_tag="PC472_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2729051.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4098..4820
                     /gene="hisA"
                     /locus_tag="PC472_003779"
     CDS             4098..4820
                     /gene="hisA"
                     /locus_tag="PC472_003779"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2729052.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4802..5560
                     /gene="hisF"
                     /locus_tag="PC472_003780"
     CDS             4802..5560
                     /gene="hisF"
                     /locus_tag="PC472_003780"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2729053.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5588..6256
                     /locus_tag="PC472_003781"
     CDS             5588..6256
                     /locus_tag="PC472_003781"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2729054.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2579 a          537 c         1250 g         1947 t
ORIGIN      
        1 aaatatagta aaattaaata caaaattaaa tcatcaccat catcatagat taaagggatt
       61 gtaatatgaa attaattcat agacacaagc cctgttttta gtgtaattgg tcttgtatct
      121 atgatgggaa gtaattttaa gagaaaatta tagccatata ggtataaagc ctatatggct
      181 tttttattgt tttaaatttg caaatataag agtaaattta atgaagaggg tgagaatgtg
      241 aaatttttga ggattgaaga tgaggttatt tattctaaaa aaagatataa attaaaaagg
      301 gaaatagata aacttttttt ggatgaagaa tattttcaaa tagaaccaca attatttgaa
      361 gaatatgatg aatttacaac tataaataat aaaattccag aagaatctat ggtaaaggtt
      421 gtaaatggaa aagtaatggt acttagagct gatataacta caagtataat aaaaagcctt
      481 gtaccaagat gggaagatgg tttaaagctt aagctttttt ataattcatc aatatataaa
      541 aataaaaata cagttggaat aaaagagata agacaaatag gttgtgagta tctaggagaa
      601 gcctctgtag aagcagatag agaagtagtg aaattagctc ttaagatact tgaaaaatac
      661 aataataatt ttattctaga ggttgggagt agcaagtata ttcatggttt gttagaagaa
      721 ttaaatttaa ataagaattg tgaaaatcaa attaaaaatt tactatatac gaagaacact
      781 catgaattaa aagtatatat tgaagaatta aaaattaagc gtgaagtaaa agagttatta
      841 tcaaatattt taagtttaca agggaattta tataatgtta ttgaaaaatc aaacaagttc
      901 tattgtaata ataagatgaa gcaagctctt gaagaattaa agcaagtaaa taatttgatt
      961 gaggaatgta attttttaga taaggctaga tttgatttat caatgattac tatgcttgac
     1021 tattatgaag gaattatgtt tagaggatac tacccaaact catataaaga aattcttagt
     1081 ggtggaagat atgattcttt aactaaagag tatggaaaag aaattccagc cattggattt
     1141 acactaagtg ttgatgagtt aatgaagtat gtatataagt aaagtttaga aggagggatt
     1201 gctttggatt atttgactat agcactagca aaaggacgaa ttgaagggga atcatttaaa
     1261 aaatttaaaa aaatgggtct aggagatagt atagacacag atacgagaaa actaattttt
     1321 aaagatgaag aaaataaaat tatatatata catgtaaagc catcagatgt agttacatat
     1381 gttgaaaaag gagtcgctga cctcggtata gcaggtaaag atactatttt agagaatgag
     1441 acagatgtat atgaaatata tgatttaggt tttggaaaat gtaaatttgc tgtagcagga
     1501 ttaaagggag atagcatata tagagaagat gaatatttaa aagtggcaac aaagtatccc
     1561 aatatagcaa aaaagtattt taaagagaaa ggtcaaaaaa ttgaaataat aaaattaaat
     1621 ggttctgtag aactagctcc aatagtaggg ttgtcagatg taattgtaga tatagttgag
     1681 actggcaata cacttaaagc aaatggtctt gaaatattag aagacatatg taatataagt
     1741 gctagaatta tatcaaatag agctagctat agatttaaat atgaacaaat tcagaatata
     1801 attcgtttat ttgaagaact tgataattaa aatataattg agctttataa ggagggattt
     1861 aagttgagag aaaaagaaag cataagagag ttaaggggat atgaaccaaa tcacgtaaac
     1921 tgtaaagtaa aacttgatgc aaatgaagga agtaagaggt tgtttaaata ccttattaaa
     1981 gaaatttcag atagtgatat agatttaaat ttatatcctg aagattctta tagtgatttg
     2041 aaagaatcta taatagatta cataaatatt tctggagtaa ataagaaaaa tcttctagta
     2101 ggaaacggtt caagtgaaat aattgattta ataatacata cttttgtaga taaagatgaa
     2161 gtaatattga gtttctctcc aagttttagt atgtattcta tatatagtca gataaatggg
     2221 tctaaattta taggtgttga aagtgatgaa aatttagtta taaatataga taatgtgatt
     2281 gaaaaagtaa aggagaataa tcctaaaata gtaattgtat gtaaccctaa taatccaact
     2341 ggaacaatat taaagaggga agagataata aagcttttag attcaactaa tagtctagtg
     2401 gttttagatg aagcatatat ggattttgga gaagaaagca tgttaagtga tgtttttaag
     2461 tatgataatc taatagttct tagaacttta tcaaaggcat ttggattggc tggaataaga
     2521 acaggatata tgttatctaa tagtagttta ataaattctg ttgagaaagt aagacctcca
     2581 tataatttaa attcattatc agactttatt gctacaagag cacttagaaa taaagatgtg
     2641 gtaaaggctt atataaagga agtaaaagag gaaagagaag ttttatatga agaaatgatt
     2701 ggtatgggaa ttaaagcata caaatcacaa gcaaatttta tattatttta ttctgaaatc
     2761 gaaaatttat cacaaaaact tattgataga ggagttttaa taagaaaatt tggtggaaag
     2821 ttagaaaatt attacagagt tacaattggt gataaagaag aaaattctat gtttgttgga
     2881 gctataagag atattttgaa aaaggagaaa taggtatgag aatttggaag gtagaaagaa
     2941 atactcttga aacacagatt ttagtagaat taaatattga tggttctggt aaagctgaaa
     3001 tagatactgg aataggattt ttagaccata tgcttacctt aatgtcattt catggcaagt
     3061 tcgatttaaa agtgatttgt aagggtgata catatgtaga tgaccaccat agtgtagaag
     3121 atataggtat agctataggt gaagcattta aaaatgctct aggtgacaaa aaaggaatac
     3181 gaagatattc taatatctat atacctatgg atgaatctct atcaatggta gcaatagata
     3241 taagcaatag accttatctt gtatttaatg ctaaatttga tacccaaatg attggtagta
     3301 tgagtactca atgctttaaa gaatttttta gagcttttgt aaatgaatcg agagtaacat
     3361 tacatatcaa tctcttatat ggggaaaatg accatcataa gatagaaagt atttttaaag
     3421 catttgcaag agctttaaaa gagggtagtg aaatagtttc taatgagata gcatcttcaa
     3481 agggggtttt gtagaatgaa tataatagta gactatggtc ttggaaatat agattctgtg
     3541 tctaggggtt ttagaaaagc tggaatagaa actaaaattt ctagtgatat agatgaaata
     3601 aaacaggcag attcacttat acttccaggt gttggagctt ttagagattc tataagtgcc
     3661 ttagataaac taggattgat accaataata aaggagcatg tttctaaggg taaatttatg
     3721 ataggtatat gtctaggaat gcaattactt tatgaaaaaa gttatgagta tggagaatat
     3781 gaaggattag gacttataaa aggaagtatt gataaactgg atataagttt aaaagtgcca
     3841 catatgggat ggaataatct aaaatttaat aaggcaaatg atgatatact taaatatata
     3901 aatgaagatg attatgtata cttcgttcac tcatattatg caaattcatc aaatgaagaa
     3961 ctaattgcat tttcagaata tgaaaagaaa atccctgcta ttgttagaaa aggcaatgta
     4021 tatggtatac aatttcatcc tgaaaaaagt ggagaagtag ggttaaatat attaagagca
     4081 tatggggaga tgataaaatg ataatatttc cagcaataga tataaaagat aataagtgtg
     4141 taagactcac acaaggagaa tttgacaaag taaatgtata ttatgataat ccattagaag
     4201 tagcatataa atggaaaaat gaaggagcag aatatataca tatagtagat ttaaatggtg
     4261 ctagaagtga atttggtgta aatacaaaaa taattgagga catagcaaac aatatagata
     4321 tacctattca agttggcggt ggagttaggg ataaggaaaa agtcaaaagt ttaataaatg
     4381 ctggtgttac tagagtaata ttaggcagta tagcgattga aaatttgaat ttagttgaag
     4441 aattggtcaa tgaatataaa gagaaaattg tagtttctat agatgctaaa gatggaaaag
     4501 tagcagttag gggatgggaa gttgtaagca atgtagactc attaacatta tgtaaacaac
     4561 ttgaaaagat aggtgtacaa actattgttt atacagatat atcaaaagat gggatgttgc
     4621 aaggtccaaa ttttgacata tatgaaagaa tagcaaagga aacatcacta aatgttattg
     4681 cttctggagg cgtaacttct atagaagacg taaaaagact aaaagctatg aacttatatg
     4741 gtgcaataat aggtaaagcg ctttatgata aaaagataga ctttaaggag gcacaacaat
     4801 tatgcttact aggagaataa taccttgtct agatgtgaga aatggaagag ttgtaaaggg
     4861 gaaaaaattt aaagatatag tagatgttga tagtccagaa gtacttggaa aattttatag
     4921 tgattgtggt gcagatgaac ttgtattcta tgatataact gcatcaaatg aagagagaaa
     4981 gacatcttta gaatttgtga caaaagttgc agaaaatata aatataccat tttgtgttgg
     5041 tggaggagta aataaattag aagatttcac tgatatttta agaaaaggtg ctgataaagt
     5101 ttctataaat tcatcagcag tcaaaaatcc tgaattaata agggaagctt cattaaaatt
     5161 tggagcacaa tgtgttgttt tatcgataga tgcaaagaaa aatgaagagg gttcatggag
     5221 cgtatacgta aaaggtggaa gagaaaaaac taatctggat gcaattgaat gggctgttaa
     5281 aggtgtagaa cttggagcag gagaaatcgt tgtaaatagt atggatgaag atggaatgaa
     5341 aaatggatat gatatagaat tattatcaaa gataacttct ttagtaaatg tacctgttat
     5401 agcatctgga ggcgctggaa aaaaggaaga tttttatgag gcggttaata aatccaatgt
     5461 agatggaatt ttagcagcat ctgtttttca ttttggcgag ataaaaataa acgatttaaa
     5521 gaaatattta aaagacatgg gtgtagaagt aagattataa gttaaatact aaaaggagat
     5581 tagaaatatg gataataagt gtaacaatgt atacagtgat gaagtagaga aatttataag
     5641 aagtataaag tttgatgata aagggttagt ccctgttgtt gttcaagagg tagttagcaa
     5701 agatgtactg atgttagcat atatgaataa agatgctata aagaaaacct taaaagataa
     5761 agtagcttgc tattttagta gaagtagaca agaactttgg gttaaaggtg agacatctgg
     5821 taatactcaa aaagtagtta agatgtctta tgattgtgat gtagatacta tactactttt
     5881 tgttgaacaa actggtgtag cttgtcatac aggcaattat tcttgttttt acagagactt
     5941 gtttgatgat agttcaaaaa tggagcttga agtacaaaca aatatattga aagaattata
     6001 tgatttaatc aatgaaagaa aaaataatcc agttgaaggt tcttatacta attatctttt
     6061 tgaaaaagga atagataaga ttcttaaaaa agtaggtgaa gagagtagtg aggtaattat
     6121 agcatcaaaa aataccgata aaagtgaact tatttacgaa attagcgatt tagtatatca
     6181 tacattggtt ttaatgatag aaaaaggtgt tgaaattgat gaaataaaaa aagaattact
     6241 taaaagaaga aaatagaata agatagatta atgggtaata agaaaatata ccgcttttaa
     6301 taaaaagtgg tat
//
LOCUS       DAMJYC010000166         6307 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJYC010000166 DAMJYC010000000
VERSION     DAMJYC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.173"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            200..745
                     /locus_tag="PC472_003782"
     CDS             200..745
                     /locus_tag="PC472_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2729055.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            758..1771
                     /locus_tag="PC472_003783"
     CDS             758..1771
                     /locus_tag="PC472_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2729056.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1993..3966
                     /locus_tag="PC472_003784"
     CDS             1993..3966
                     /locus_tag="PC472_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2729057.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4035..4394
                     /locus_tag="PC472_003785"
     CDS             4035..4394
                     /locus_tag="PC472_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729058.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4429..4785
                     /locus_tag="PC472_003786"
     CDS             4429..4785
                     /locus_tag="PC472_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2729059.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4822..6114
                     /locus_tag="PC472_003787"
     CDS             4822..6114
                     /locus_tag="PC472_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2729060.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2500 a          588 c         1185 g         2034 t
ORIGIN      
        1 attctaatta gtacaatttt ttcctttata aaattgaaaa aagatgtatt tatttatcaa
       61 aaatattata ttaaaatgat attaataaaa aaacatttac aatattagca tatagtacag
      121 aaaaaagatg taatttgtaa aatttatggt aagcagtaaa attaaaaatt taaagatatt
      181 atagggaggt tataatttta tggaaaacgt tattacaggt ctaagtaaag gtgcagagtg
      241 gtttatcggt ttgtttcaga aagggggaga acaatttata ggtcttgttt caggtactct
      301 tccaacgcta attgtattaa tggtagctat taactctttg attaagataa ttggtgagga
      361 aagagttaat aattgggctt ctaagctagg aaagaactct tttacaagat atattctact
      421 tccattagta tcagtatttt tcttgggaaa tcctatgtgc tatacatttg gtagattttt
      481 aaaagaagag cacaaagcag gattttatga tgcagcagtt tcatttgtac atccaattac
      541 aggtcttttt ccacatgcaa atgcaggaga actttttgta tggttgggaa tatctgcagg
      601 tttgactact ttaggtaaag aaactactac actagcattg tggtatttta tagttggtct
      661 aattgtaatt tttataagag gtatagtaac agaaaaaatg tatgcttttc taacaagaaa
      721 aaatagagca acaaataagg cttaggaggt agataaaatg gaggagaata aaatattaaa
      781 aatagaaaag ggaacttcag gctggggagg tcctttgtat ataaaaaaag aagggaatag
      841 aaataagata ctatcaatga ctgctggtgg tatccatgaa gtaacattaa aaataaaaga
      901 actactgggt tgtgaaattg tagatggatt taaaactgga gtttctgatg aagaagtagc
      961 agtagttatt attgattgtg gtggaactgc tagatgtgga gtttatccaa agaaaaaaat
     1021 acctacaata aatgtaaatc cagttggaaa aacaggtcct ttagcaaaat ttataacaga
     1081 agaatattat gtatcagatg ttaatcctaa ctgtataagt gttgtagatg gggaagatat
     1141 gccacaaaaa tctcaagaaa ataaaagcga aaataagagt tctattagaa agcctgataa
     1201 ttatgatgag gttaagtcaa aagcacaagg tgaatatgct aaaaagaaca taatactttc
     1261 tattggtcaa ggggcagggc aagtagtatc aaaattttat gatgcaggtc gagatactat
     1321 ccaaatggta atgaataacg taataccatt tatggcattt gtaagtatgc ttatgggaat
     1381 tattttagca tctggactgg gagactggat tgcaagagtt atatctccac ttgcaggtaa
     1441 tattggtgga ttattgatta tatcagtaat ttgcacactt ccattcttat caccaatact
     1501 aggacctggt gcagttatag ctcaagtagt agggactctt gtaggtacac aaattggtct
     1561 tggtgcaata ccagcatatt tagcacttcc agctttattt gcaataaatg ggcaagctgg
     1621 atgtgatttt gtaccagtag gattaagttt gggagaagca gaaccagaaa ctgtagagta
     1681 tggtgttcca gctttatttt actctcgttt aataactggt ccaatagctg ttattattgc
     1741 atatggagtt gcagtatttg cattgagata atatttaatg tcaatataag gcttggaatt
     1801 aattaataaa attgtaaata taaatttaag ctagtcaaga ttttttgtta atgaaagact
     1861 agcttttata ctattaatta gctgtaatta tcgagttaga ttaatactaa attttccttt
     1921 attaaagtga aattttatgt attaatttgg tttcaaattt gtactagaat gaaatcaagg
     1981 aggagataca aaatgagtgt aactgagaaa attccttcta ttgaccgcaa gatatttaag
     2041 atattgatta tgtgtagtaa aaaagagttt gtgagtatta actcaatagc aaatgaattg
     2101 aatgtcacaa ctagaagtgt aaggacgtat ataaaacaat tgaataaaga tttaggtaat
     2161 gatatagcag taatcaaata tataaagggt caaggctata aactagaaat aaaagatgag
     2221 cagatactca ataagattat tgatatgaat agaaaaaatg tattttcatt aaattcaaaa
     2281 gaagatagag tagagtttat actaaattac ttgatagaac tagatggatt tattacactt
     2341 gatagtttag ctgatgagat gtgtgtagga agaactactt tagtaaatga tttccagtat
     2401 gtagaaaaag ttttggcttc atataattta aacttgatta aaaaacagaa tacaggtatg
     2461 aaattaaatg gaaatgaact tgatattaga ctgtttatat taaatcagtt gtataaaaac
     2521 tcaaggaaag atttcagtaa ttcaaagtat tttaaaggaa tcaaaaaaga agaaatcata
     2581 aatttagaag aaaaactatt gactttattt aaaaaaaata atttttatgt tacagatgaa
     2641 atgttaagag aagttattaa ctacatagta gttttagtct atagagtaaa agaagaaaag
     2701 aaagtaaaag attatgatgt aaagtttgat ttacttaaat catatgatga atactttatt
     2761 gctagggaaa taaaacatat aatatcagga atgtttgaat gtactttaaa tgatgaagaa
     2821 attatttatt taactatacc gttagttagt ggaaatgcac cagcatcaga atgtgcttta
     2881 aatagtagta gaattagtaa aaacattgat gagttgatgg aaaatatatt taatcaaata
     2941 tatgtcgata tgggtatttt tataaatgag gatgaattaa gagtaggtct aggatatcat
     3001 cttagtttta ctttaaacag gctattattt aatataaagt taaaaaatgt tttgttagaa
     3061 gagattaagc aaaattatat acttccattt aaattagcac aaatagcagg aaaagtaata
     3121 gagaaaaagt ataacttaga agtttcagaa gatgagattg gatatatagc aatccatttc
     3181 agtggatatt tagaaagaaa tagcagtaga ttctattcaa taaaaaaaat agcaattata
     3241 tgtagtacag gtctaggaac agcaaaacta ttgaaaataa gagtagaaaa actgatagga
     3301 aataatccta aaattgatac tctttcaagt tttaatttga gaaatattaa cttagatgaa
     3361 tacgatatag tttttacaac tattgattta gacacatcaa atataaatac aatagtatta
     3421 aaaataaata caatatttga tgaaaacaaa ctaagagaac aattaaaaac agtattgtgc
     3481 ctaagagaag ggaatattga cactactaat tctacaaatt tactaataaa taatttgtta
     3541 gatgaagata aatttatgat attaaatgaa aagactatac ttgcttcatt agaaaaaatg
     3601 atggaccatc ttatggattt gggatgcatt gatgataagt ttagaaaaaa tatagtcaat
     3661 agagaagaaa aatctcccac agtatttgat aaaggtttat tatttcctca ttctgtaaat
     3721 gaaaaatcag ataagttttt aatggcagtt ggaattttag aagaaccaat agaatatgca
     3781 aatagaagta taaaaataat tcttatgaca atgtttccat gtgagaataa gatagactca
     3841 gacttattag taaaaattta tgaagaagtt ttaaagatag gccaagatgt aaaattaacg
     3901 aataagataa gtaaatgtag aactttttta gaatttaaaa aggtgttact taaaaacctt
     3961 atataactat tgtacagcat ttatagttgt tatacagatt taaaaataat gtgatttttg
     4021 gaaggtgtat aaatatgaaa ttattagtga ttttatttgt tgcttatatt ttaaatacta
     4081 tatttataat gaagcagaat aaattgtatt ttaaaacttt aaatagagct aaaaaaaagg
     4141 gtgatatagt gtctacaggc aaaaagaaaa gctatttttc taaaggaagt atagccatta
     4201 tatcttcaga ttcagatggg tttataaagt ttggagaaat tttaaaagga cgtacagtaa
     4261 tggctaaatt taaagagatt gaagaaatag aaggattaga tatatatagt gcagagcaaa
     4321 aatttaaaga tgaagagagt attgtacaag ctataaaatt tataaaagaa aaaataaatg
     4381 tttcttttaa ttaactcaat ataaatataa attatcagga ggaatgtcat gaaatatgaa
     4441 gttaagataa gtgggatagg cagtttagtt aaagagttaa tggatgaaag caattgtctt
     4501 attatatatg atgaaaccat taatgatgac gacttaaagg atatatctgt aattcattca
     4561 atagcaacgt taaaaagtga tgttgaaata ggagatacac ttactatagg gaatagagat
     4621 tattgtatag tttcagttgg tgatattgct cagaagactc ttagagaaat tgggcattgt
     4681 acaatcaaat ttgatggaaa atgtgaagta aatttaccag gagaaataca tgtggaaata
     4741 ggaaatccag atataactat tggagattta ataactattt cttaatttga aaataaaatt
     4801 attataacag gaggaattgg tatgttagat ttaaacagaa aacttgcaaa attggaagag
     4861 gaaggtaaaa aaataaaagt agctcttata ggagctgggc atatgggaaa tggaatggta
     4921 agccaaatgg caaatatgaa aggtattgaa gcttctattg ttgtagatat aaatttggag
     4981 ttggctcata aagcttttac tgatgcagga atagatgaag aaattataga taatgtaaca
     5041 aatgaaaagg atgcagaaat aaaattacaa gaaggaaaag tattgacatg taatgatttt
     5101 cttgtggctt gtaaaactaa gtcaatagat gtagttatag atgcaactgg aggtattgca
     5161 attggtgcag agatagcatt aaactctatt ttaaataaaa aacatatagt tatgttaaat
     5221 gctgaaactg attgtgttgt tggaccaata cttaaaaaat tagcagatga tgctggagta
     5281 atatttacag gttctgcagg agatgagcca ggtgcagtaa tggaattatt tgattttgca
     5341 gatgcaatgg ggtttgaagt tagagtgata ggtaaaggta aaaataataa attagattta
     5401 gattgtaatc ctgatacagt tagagaagaa gcagaaagaa aaggagcttc acctcatatg
     5461 atagcttctt ttaaagaagg aactaaaaca atggtagaaa tggctttaat gtgtaatgca
     5521 actggatttg tgcctgatgt aagaggtggt catggtattg aagctacagt taatgaagta
     5581 cctaagaaat atgcattaaa aagcgaaggt ggagtattag ataattatgg tgtagttgat
     5641 tttgtaaatg gtatagcacc tggagttttc gtagtagtag cccataaatt aaaagcagtt
     5701 aatgatgaac taaaatattt aagtatgggt gatggaccta attacatatt atatagacca
     5761 tatcatttat gtagtttaga gactccttta tcagcagcta tggcagtctt agaccataag
     5821 gctacaatag tacctaaagc tggactagta gcagaagtta tgactatagc taagaaagat
     5881 ttgaaaaaag gtgagtttat ggatggctat ggagcatata catgctatgg tactatagaa
     5941 aaatacgatg tagcaaaggc tatgaatgct gtacctattg gtttgataag taaaaagact
     6001 aaagttgtaa aagatattaa aaaaggtgaa gttataactt acgatatggt agagatagaa
     6061 aaagatacta ctttatatca tttaagacaa ttgcaagaaa aaatatttgg gtaagatttg
     6121 atggtaatat aaatagatat tagtaaaaag ggttgatggt tatttttaaa tcaacccttt
     6181 taaattttta tatattcttg ttaggatact ttctttaaaa gtatattata ggttatttgt
     6241 agtcgtaaaa attattttga attagttctg caattttata ttcacattga aataatatta
     6301 ttaaaaa
//
LOCUS       DAMJYC010000167         6166 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJYC010000167 DAMJYC010000000
VERSION     DAMJYC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.174"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(129..536)
                     /locus_tag="PC472_003788"
     CDS             complement(129..536)
                     /locus_tag="PC472_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729061.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1010..1894)
                     /locus_tag="PC472_003789"
     CDS             complement(1010..1894)
                     /locus_tag="PC472_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2729062.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2185..2661)
                     /locus_tag="PC472_003790"
     CDS             complement(2185..2661)
                     /locus_tag="PC472_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2729063.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            2928..4103
                     /locus_tag="PC472_003791"
     CDS             2928..4103
                     /locus_tag="PC472_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2729064.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4184..4987)
                     /gene="proC"
                     /locus_tag="PC472_003792"
     CDS             complement(4184..4987)
                     /gene="proC"
                     /locus_tag="PC472_003792"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2729065.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5027..5611)
                     /locus_tag="PC472_003793"
     CDS             complement(5027..5611)
                     /locus_tag="PC472_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729066.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2055 a         1037 c          688 g         2386 t
ORIGIN      
        1 ttagctttag gggtttccag caattcaagt agtattggat agattctatc ctatctctac
       61 atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa agtcacgagt
      121 gtgcgtagct attttttaat caatatttta gtaaatttta cggtaagtaa aaacctctct
      181 ccttcaaatc ctatagcaaa tatacttaca agttgctttg agaaaataac tcctaatata
      241 gctacaacta tgcatatacc aactattata tttaaaacat tgtttaaaaa ctttagtgct
      301 tgcttttcac cttgtttact acttatatct tgatacattg gaataaaagt agtaactata
      361 gcagtaccta ttgctgcaaa tataatattt ggtatattca tagctgttaa ataactctct
      421 gtatataagc ctgtaccata aattgatgat aatactaatt ctctacccat tccaagaatc
      481 ttagagatta tagtaactat catcaaataa aaagttgctt tagctacctt gctcatttta
      541 tcactccact tctaacatac ctctttttaa tcaagtaaaa tttagctcat tagatataat
      601 tctatcaact aatttgtctt gcttagccct atcaaaatat aattaacacc tatttcttaa
      661 tatatcacaa actacattat tcttcatatt tttttatttc tccaataagt tttacatctt
      721 tcctatataa attgtatagc ttcaattata ttcaaagaaa tggagttgct acccgataac
      781 tttaaaagta aaaaacagat tttattatct ttttaaacta tattctaaca tattttgtcc
      841 atattgagaa attttactaa actattatgt actttattaa ataaataaaa taacagcaca
      901 cctataaaat acaagtgtgc tgttcataaa cagattttat aaaaaaataa ttcctaagta
      961 gagattaaca tgctttctca tactctcata tataatgtgt ataaaaatat taataaaaat
     1021 gctttaattg ttttaactta cttggatgtc ttaattttct tattgcttta gcttctatct
     1081 gtcttattcg ttctctggta actccaaata cctttccaat ctgttctaat gttttagggt
     1141 catcatcatc aattccaaat ctcattctta aaacttgctg ttcttgctct ccaagacctg
     1201 taagtagttc ttctatcttt tcttttaaat tatgctgttc aacttcgtgt attacaacat
     1261 ctttaaaatc agcatctgat ggtataaatt caactaaact actatcctca tcttctttta
     1321 atggagtatc caaagataca acatttttat ctcttcttaa taattccaac actttgtcaa
     1381 cttctaagcc acatgcatct gcaatctcat ctatagttgg ctctcttaat aatacattta
     1441 aaagttcttg tttctttttc tttacaaaat ttattctctg atgaagatgt ataggtatcc
     1501 taatagtatt ttcgcaatcg tcgatatatc ttgttatact ttgttttatc caccaagttg
     1561 cataagtgct aaatttgtat ccttttttat aatcgtactt ttctacagct tttataagtc
     1621 ctatatttcc tgcttgaatt aaatccaaca tatcaatact atctctttta tatctctttg
     1681 ctatgctcac taccaaccta taatttgaat ttataaattt ttcttttgct actgaagatg
     1741 agttgattat ttccttagcc agctctacct cttctcctgc tgacaacatt ttgtattcct
     1801 ctatttcctt taaatacatt ttcatcgcat ttgatacatt tgatgaaccc acacaaatat
     1861 aattttcata attattttct tttttagtat ccataatcaa accccttcta ttcatagaat
     1921 ttgctaaaac aaaaataaat aatttaaacc tacataatct acaattacat aatatcccat
     1981 tctttaaatg aattgttttg tatattatgt atacaaattt ataaaaacta tacctataat
     2041 attattctac ttctaacagc aatatcctcc ttatatatat aatttatttt attatatgtt
     2101 gttatattct tagttttttg aatgatatat gtctaaaaaa agactgttta ctaaacagtc
     2161 tttttttata tatatgctca atatttaatt ttcatgatga tgattacatc cacatccaca
     2221 attttcatca tggtgatgat gttcttctaa atcttttgcc tgttgttcca tttcatgaaa
     2281 ctcttttacc attatatcat aagttggaag agctgcctca gtatcgtatt cttcccctct
     2341 ccaatcagcg tacatcatag catctccatt agttaaaacc aatctacctg acatagttat
     2401 attatccact tcttctaaaa attctatttg tttttgtaaa acttctcctt ctaactccac
     2461 atcttcatct aaacattgca ctatcattac aggtgcataa taatcttctt catctttaac
     2521 atttacaaca atatctaaaa ttccttctgc accttcttca aattctgcca actcaacatt
     2581 tgtatcaatc atatcttgag gtactaataa atcttctatt aaatatttta ttactctatc
     2641 ttttactaat tcttttgaca tttatttttc tccttccgat tctataacat tatcataatc
     2701 atttctatat tatagtatac tacattaatt gttttttcaa tctcttacaa aaattatttt
     2761 tttattatct tccttatatt ccctttttta ttacaaagtt gtaatcgcat tgatttaaaa
     2821 aatagtttat atttagtata atagaaatgt agcacatatt tttattcaat atattaataa
     2881 aaaaatatat tgaaaatgta atgtatgtta aatctaggag gaatattatg aagtttaaaa
     2941 aacaaattag ttctattgtt gtgtctacta tgcttgtact tgggagtatg aacctttctt
     3001 atgcagacgg tacaaatgta gttacacttg gagctaactt atcagagtct caaaagcagc
     3061 aaatgcttaa ttactttgga gtaaaaaaag accaagtctt agttttagat gtaacaaatg
     3121 cagaagaaag acagtattta caaggtgttg caacagaggg tcaacttgga aaagttacta
     3181 tctcttgctc atacgttgaa cctactaaaa aaggaaatgg aattaatgtt aaaacagcaa
     3241 acttaacttg ggtaactagt tcaatgattg cttcaaccct tacaacagct ggtcttgaag
     3301 atgcaaatgt tattgctgct gctccatatc ctgtaagtgg tactggagca ttgacaggaa
     3361 tcatgaaagc atttgaggat gcttcaggaa agaaacttga tgaaactaag aaagaaattg
     3421 cttctgaaga attggtagtt acaggtgact taggagatga tatagggcaa gaaaaagcaa
     3481 caggtgtaat gaatgatata aaaacagaaa ttgtaaagaa tggtacaaaa gataccaatc
     3541 aaatagcaaa tacaataaat aatgttgtaa ataattacaa tattactatt acaccagaac
     3601 aaaaagaaca aattaaaggt gtaatgaaaa aaatagctga ccaaaattac gattacaata
     3661 gcatgaaaaa tacactagat aatgtatcag ataatgtaaa tgaacaatta aagaaattag
     3721 gtgaaagtgt taagggttct ggtatactag atactattgg tggatggttt agtggaatag
     3781 gagactggtt ctctggttta ttctcaggcg gagataaaaa agatttggga atcttaaata
     3841 acactaatga tgagtctctt ggctctgatg ctgtaataaa ttctacagaa ggtgttcaaa
     3901 taaatccaca aaataatact aattctaaca atgatagtga tacaaatagc tcaaaaaatg
     3961 atacttcaag taataatgaa aataacgata gttctcaaac aaatgaaaac aacacaacaa
     4021 ataattcaga aaatcaatca aataataatg actcagttgg tttctttgaa aaaataaaaa
     4081 attggctttc aggcttattt tagaaattaa acaaataaaa agaattgcct aaaatggaaa
     4141 ttttctataa aaggcaattc tctttattta caataaattt ttattactca ctcatttttt
     4201 tagatttttc tatacatgca catattgctt ttatcacaga agctctaaaa ccttcttctt
     4261 caagtacttt aactgcttct atagtagttc caccaggtga acataccata tcttttaatt
     4321 caccaggatg ttttcctgtc tcaagtacca tttttgcaga acccataaca gcctgtgatg
     4381 caaacttata tgcttgtggt cttggcattc ctgcaattac tgctgcatct gctatagctt
     4441 ctataaataa aaatacatat gctggtgcag aaccacttgc accaattaca gcttctataa
     4501 gatactctgg tactacctca gtatttccaa aagaattaaa tacttctgta actgcttcta
     4561 agtcttcttt gtttatattt ttatttatag aaagagaaga catggcttca tttacaagag
     4621 ctggagtatt tggcatagtt ctaactattt ttttatcttc tcctataata gactctatat
     4681 cttttataga ttttcctgct gctattgtaa ctataatttt attgtcatct ataaagtctt
     4741 ttatctcttc caaaatatca tcatatatat ctggtttaac agctacaatt actatatcag
     4801 aggttctagt tacttccttc gagtcttttg tagtatttat tccaaaattt gcatctattc
     4861 tatcgagagt tccttgacta tatgctgatg ctataaccat attaggttca actaattttg
     4921 aatttactat tccccctatc atagcgcttg ccatatttcc tgcacctatg aatccaattt
     4981 ttttcacttt agtacctcct tattgactaa tagtaatcgt taaaatttaa gcttctttaa
     5041 ctgcatcaga cttttcaaat ggatttggta tacttactac caaaattttc actaccttat
     5101 tagtatagtt gacccaatta tgattgttct tttcactatt atagtgtatt gtgtctcctg
     5161 gatacatttc tatttgttca tctcccaaaa acactgtaag agtaccctct aatacataca
     5221 caaattcctc accttcatgt acgtaacaac aaatttcctc actactctta cttggaagaa
     5281 tttcaattaa tcttggtagc attgtttttt cctttaaact agatgataaa tgataatgta
     5341 taaatgttga gttttctaca tcaaatactt ctttttcata acttcttaat acagctcttt
     5401 tatgctcttt tggcttcata aaaaagtaag ttaattctac atctagtaca cttgctattt
     5461 ttcctaagga atctgttgcc acacttgtta atcctctttc aagttgtgat aaaaaaccaa
     5521 ttgacaagtt tgttttttca ctcatatcct tcagcgtcat ttgcttttga gttctgagtt
     5581 gctttatttt tgctcctatg tccttattca aaaccatcac ccctaaataa ttttaatttt
     5641 ttcataatat aaatacaaaa atataaatat aggtactgaa cctatcttta tttcatttaa
     5701 aaaaattttt ataagtatta ctataattta tcaatctttt tttgtctaca tttttacgat
     5761 tatttacgat ttatttttca catatgtata tttaatcttg ccaactaaag atacattttt
     5821 tataagcatt tactctttat ataataaaac ttaagttgtt catgtagtct tcttctggtt
     5881 taattattaa aatttcaaat tatattatta tttctacaca ttattacatc ctttagtaca
     5941 attataacat aattttacaa tttaatgata tctattcaat gtcttttttt actctacaac
     6001 atattttatt ttagagttta atactatata tattttttat atacagcctt gtcatttttt
     6061 agattgtcaa gagttattga agtattttat gtagatttag attctatttc ctaatttctc
     6121 atttaacaag aattgcttat gataaaaaat aatatcatct caataa
//
LOCUS       DAMJYC010000168         6122 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJYC010000168 DAMJYC010000000
VERSION     DAMJYC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.175"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            296..862
                     /locus_tag="PC472_003794"
     CDS             296..862
                     /locus_tag="PC472_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2729067.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            902..1483
                     /locus_tag="PC472_003795"
     CDS             902..1483
                     /locus_tag="PC472_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2729068.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1540..2457)
                     /locus_tag="PC472_003796"
     CDS             complement(1540..2457)
                     /locus_tag="PC472_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2729069.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2587..4218
                     /locus_tag="PC472_003797"
     CDS             2587..4218
                     /locus_tag="PC472_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2729070.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4221..5168
                     /locus_tag="PC472_003798"
     CDS             4221..5168
                     /locus_tag="PC472_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2729071.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5453..5716)
                     /locus_tag="PC472_003799"
     CDS             complement(5453..5716)
                     /locus_tag="PC472_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2729072.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5815..6060)
                     /locus_tag="PC472_003800"
     CDS             complement(5815..6060)
                     /locus_tag="PC472_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729073.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2214 a          834 c          994 g         2080 t
ORIGIN      
        1 tccaaatccg ttctcattta gatagtatgc ttagaaattt tagaattatt gagtctaact
       61 ttattactta cttcacttta taacaattaa atacaaattt aaatattata aaactacaaa
      121 ataatttaaa gcatataatg tcaatatgtt taaattataa aatattcatg tagttatata
      181 ataaataatt tataaatata gccaacttag cctaaatacg tatataatat agctaatatg
      241 gttcttatac ttgataccat caataaaact aaaaatataa aaaggagaac ttaaaatgag
      301 tgttagttta caaaaaggac aaagagttag cttagtaaaa gataataatc ctgtaaaaaa
      361 tctagtagta ggcttaggat gggatatgaa taaattgggt aaaaaaaatt atgacctaga
      421 tgcatttgca atagcattga ctaaccaaga taaaatgaat gttagaggtg acttggttta
      481 ttttggaaat cttaaacacc cttctaaagc tattatacac actggagata acttaactgg
      541 aaaaggtgaa ggtgatgatg aacaaataat tgttaattta gaagacatac ctgaatatgt
      601 acataaaata gtttttggag tttctatata caaagctaaa aaaagagacc aggattttgg
      661 tcaaatgaac aactctttta taagattaat tgattcaaat tccaagcaag aattatttaa
      721 atataatctt caagaagact tttctggtaa agtaacagtt ttggcaggag aaatttatcg
      781 tagaaatgaa gaatggaaat ttaatgcttt aggaattggt caaaatgaag agcttcgtga
      841 attaattaat acatataagt aataattaca ataaaaaatt tataatgagg agttggttta
      901 aatgggtata actttagcaa aaggacaaaa agtaagttta acaaaaagta accctggtct
      961 aaaaaaagta atagtaggac taggatggga tattaataaa tatgatggtg gctttgattt
     1021 cgatttagat gcctctgcat ttttaactgg aactgatgga aaagtaacga atgatggaga
     1081 ctttatattc tataataatt taaaacacgc ttctggagct gttgaatata tgggtgataa
     1141 tagaacaggt gttggtgatg gtgatgatga acaaataaat gtagacttat ctaaaatacc
     1201 tcaaaatatt gctaaaatat catttagtgt tactataaat gaagcaatta caagaagaca
     1261 aaatttcgga caagttgaaa attcatatat aagaatatat aatgaagaaa ctaatgagga
     1321 acttattaaa tatgaattgg gtgaagattt tagcatagaa acagcaatag ttgtggcaga
     1381 actatataga cataatggag agtggaaatt taatgcttta ggttctggat ttgaggatgg
     1441 acttgcagga ttgtgtaaaa attttggcgt taatataggc taatatatct aaataaaaat
     1501 aagagtatat tgatatactc ttatttttat ttagatatat tataaactgt ttacacttct
     1561 actcttcttt ctcgcttgtt ttttcccata ttctactgaa atatcaaaaa atgctgaaat
     1621 attttctgta aaaaacttgc ttctatgata tgcaatatca aatgtcctat gtaaattaat
     1681 accttcaata tcacaagccc acaaaagatg cttttttaaa taatcctcaa taagtaattc
     1741 ggatataact gcaatgccaa aattatctac aactgctctt aatatagctt cagagttata
     1801 acatgaccat ctaatattca ttgggatttt taattctttt aattgctttt ctaattgtgc
     1861 tcttgtacca cttcccaatt cacgcataat taatggttga tttgaaagtt ctgaaacttt
     1921 tatagagtct cttttataaa actcatgctt gtgtgaacat ataagtacaa gattatcatt
     1981 aataatgctt tttgtaacaa tatctgggtg cttaattttt ccttctacaa tcgcaatatc
     2041 taattcacta ttaagtaatt tttttgcaat cagtcttgta tcttctacaa atacttcagg
     2101 ttctataaga ggatttacct tgtataattc taacattatt ggacttatta cacatgtacc
     2161 aacagttact gttgccccaa ctcttatacg gatatttttt gcacaatgct tcatacaatc
     2221 attcatttct tcatttacag ctaaaagatg cctcccatat ccaagaagtt ttttccctgc
     2281 ctcagttaaa tataattttt ttgaaattct atcaaatagc ttaacaccat actctttttc
     2341 tatttctgaa atagcttggc taactgatga ctgggtaata tacatatttc ttgctacttc
     2401 actcatttta ccacattcag caaccttaac aaagatttca agactacgaa ttgtcatatt
     2461 atcacaacct tagcaaacat tagtaaatgc ttatgatttt attaatattt tagtatttta
     2521 caaatatatt gtcaagaagt ataattttat tatagagtta taaagtagat tggagtgtga
     2581 tatattgtga aagtattaat tataggtgga gttgctgctg gaacaaaaac tgctgcaaaa
     2641 ataaaacgtg aaatgggtga taattgctct gttacaatac taaataaagg tgatgatatt
     2701 tcttatgcag gttgtggtct tccatattac gttggtaaga taattgaaga taaatcatct
     2761 ttaatcgtaa atacacctga aagtttttct aaactaacag gagcagaagt tcgttgtgga
     2821 gttgaagtta catctattga tagaaatgca aaacaagtaa ctttgaaaac gctttcaaca
     2881 ggagaaaaag aaaatctaag ctatgacaaa cttgtaattg ctacaggggc agacccaata
     2941 aaaccaccaa tacctggaat agatttacct ggagtattct ttatgcgtac tccaaatgat
     3001 gctataaaac taagagattc tatcgaaaac ggtattaaga gagctgttgt tattggtgga
     3061 ggtttcattg gtcttgaaat tgctgaaaat ctttctgcaa tgggtatacg tgtaagtgtt
     3121 gttgatatgg ctgaacatgt tatgcctgga tttgatactg attttgctga atacgttgaa
     3181 aatcatatgg ctgataaagg cataatgatt tttacaggtg accaggttgt aggtattgaa
     3241 ggtgaaaata aagttgaaaa acttcgtact aaaaatagag ctattaaaac agatttagtg
     3301 gttatgtcag ttggaatccg tcctaacaca ggttttctaa aagacacagg tttagaattt
     3361 gcccctaata atacaattat tgcagatgaa tatatgctta caaatgacaa agatatatac
     3421 gtagttggag actgtgcttt tgtaaaaaat tctttaacaa ataaacctac atggtcacct
     3481 atggggtcat ctgcaaatca tgagggtaga atttgtgcac aaaatatatc tggaaaatct
     3541 aaaacttaca atggtgttct tggaacaaca gtagtaaaat tacctgagct caatgcagga
     3601 aaaacaggtc taagtaaaga aatagctgaa aaagaaggtt ataatgtttg ctctgtaacc
     3661 atagctactg atgataaagc tcattattat ccaggtgcat ctaattttat tataagaatg
     3721 gttgctgaaa aaaatactag aaaattatta ggtgttcaag ttatgggacc tggagcggtt
     3781 gataaaatag tagatatcgc tgctactgca ataactttag gcgctgacct ttattcatta
     3841 gaaagtatgg accttgcata tgcaccacct ttttcaacag ctattcaccc ttttgtagtg
     3901 gcagtaaatg ttttacaaaa taaacttaat ggagaacttg atagtgtttt acttgacgaa
     3961 attgaggatt ttgacagttg gactaaactt gatgtttcaa aagctccttc tattcctagt
     4021 ttacgttata ttcctgttgg tgaaattaat ggagaaattg aaggccttgc taaggatgaa
     4081 aaaattcttc ttatatgtgc taaaggtaaa aatagctata tggcgcaaaa tcgcttaaga
     4141 cgctttggct atacaaatac gaaagttctt gaaggtggaa tactatttta tccaaatctt
     4201 aaggtaatgg aggagtaaat atgagtaatg ttacagcaga acaaaaaaag gaagtaaaag
     4261 gtagaggttt tctaccaagc agggatggag aacattttgc agcacgtata ataactgtaa
     4321 atggtgtaat aaatgcatca caagcaaaaa aaattgcaga agctgctgaa aaattcggaa
     4381 acggtcagtt agcatttact actagactaa cagtagaatt acctggtgta aaatttgaag
     4441 atattgaagc tctaagtgaa tttattgcta gcgaaaatct aataacaggt ggaactggat
     4501 caagggttcg tcctgttgtg gcttgtaaag gtactgtttg tgttcatggt ctagctgata
     4561 cacaggcact tgctgctgaa atacacgaag aattttacaa aaattggtat gatgtaaaac
     4621 tacctcataa gttcaaaatt ggtgttggtg gatgccctaa taactgtatt aaacctgacc
     4681 taaacgatct tggaattgtt ggtcaacgtg tacctgacta tgattcagag ctttgtgttg
     4741 gttgtaaaaa atgtgctgtt gtagaggtct gtcctgttaa agcagctaaa ttaacagata
     4801 aaggtaaact tgaaatagat agtaatctat gtaataactg tggtaagtgt atagaaagtt
     4861 gtaattttga tagtattgaa gaaaaagaat ctggttataa agtctatata ggagggaaat
     4921 ggggaaaatc agttcgtcct ggaacacaga ttgatagatt attttcaaaa gaggaactta
     4981 tgactttaat tgaaaaagca attcttttat ttagagaaca gggtaacaca ggtgaacgtt
     5041 ttggaataac tatcgataga atcggtgctg ataaatttat agaaatgctt ttatcagatg
     5101 aagttttgga aagaaaagaa gagatactag aggctctttt acaccttaca ggtggagctg
     5161 tatgctaaca ataaatattt taattgtaca gataacctat ggaaaaaatt ttagttaaga
     5221 ataattaaaa ttctatatat gatatatgat atatgaggct atctcaaaac acagatacgc
     5281 ctctttttca ttgttgaaat gataaagtat atttgagttc atataaaaaa gagtgtctcg
     5341 tgaattataa aattcatttt gaggcaccca cattatttta taataattgc ttattaattt
     5401 tattttaact atataaaatg tacaaaaagt aagaatatcc aatattctaa aattaatttt
     5461 gaattgtatt gattgaaaag tttactattc cactatttcc atcttgattt actactagat
     5521 accatgttcc agtattagga actctcattc tacatggatt tttctttgca aatccaccat
     5581 agtaatcata atttctttga tttttaaatt tcacataatt tatatggtct aacaatatta
     5641 cattggcttg tttctctaaa tttacctcta caatatcccc tttttttaag ttcatcttac
     5701 tataactata ttccattgaa ttactcctcc agtatcgaat aatctttgtt atttactcat
     5761 ctcaaatatg taaaaattca tttaaaatat tacaaatact atataattag actattatcc
     5821 agttttaacc ttatttacta caatccaaag gttattaaaa atattattgg caacaacttc
     5881 tccatcccat acaactcttt cttctttatt ttctaataaa gcatagtacc cagagtcaga
     5941 actttcatct ttatagactg taacataaaa ctcttcatca ctatattcag gtatattttt
     6001 aaagtcttca gatattttac atgttaatat ataaccttct aattcttcat ctataaacaa
     6061 agcaattcct ccaatctcta tatttttact aattatacca tattatgtca taaacttttt
     6121 at
//
LOCUS       DAMJYC010000169         6091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJYC010000169 DAMJYC010000000
VERSION     DAMJYC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.176"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            176..1501
                     /gene="cdeA"
                     /locus_tag="PC472_003801"
     CDS             176..1501
                     /gene="cdeA"
                     /locus_tag="PC472_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2729074.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1596..2390)
                     /locus_tag="PC472_003802"
     CDS             complement(1596..2390)
                     /locus_tag="PC472_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729075.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2391..3266)
                     /locus_tag="PC472_003803"
     CDS             complement(2391..3266)
                     /locus_tag="PC472_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729076.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSSDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3270..3638)
                     /locus_tag="PC472_003804"
     CDS             complement(3270..3638)
                     /locus_tag="PC472_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2729077.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3821..4480)
                     /gene="deoC"
                     /locus_tag="PC472_003805"
     CDS             complement(3821..4480)
                     /gene="deoC"
                     /locus_tag="PC472_003805"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2729078.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4516..5703)
                     /gene="murJ"
                     /locus_tag="PC472_003806"
     CDS             complement(4516..5703)
                     /gene="murJ"
                     /locus_tag="PC472_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2729079.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5780..>6091)
                     /locus_tag="PC472_003807"
     CDS             complement(5780..>6091)
                     /locus_tag="PC472_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2729080.1"
                     /translation="DLQVKNIIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVD
                     KFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQN
                     K"
BASE COUNT         2028 a          956 c          668 g         2439 t
ORIGIN      
        1 taagaaacag ttagatttta ctaatataag tttattaatt agcattatac taatttatct
       61 taatatgatt ttttgttgat tttaagctgt ttatcgtata aataaaattt ttcttgacta
      121 tatagttact atatagctta ttatatatag agagtaataa aaggaggtaa aatttatgga
      181 aaatttattt acaagaaaat tcactacttt tgaatttcta aaatttgttt ctcctgcaat
      241 tatatccatg atatttatat ctttgtacac aataatagat ggcatctttg tatcgacatt
      301 agttggttct gatgctcttg ctagtataaa tattgtacta cctataatta accttgtttg
      361 tggatttgga ataatgatgg caactggtgg aggtgctatc gtttctatac gtatgggtga
      421 aaatagacag gatgaagcca actctacatt ttcttttata gttttgtttt cattgattgt
      481 tgggatttta ttcacagtaa tctcatattt ctttatcaaa gaaatatcta ttttgcttgg
      541 tgcaacagat aagttattac catattgtat aacttatggt aaagttatga ttttatgtac
      601 accattttat attttaaaat ttatatttga gtactttgca agaactgatg gaaattctaa
      661 atttagttta tttctatcag tcattggtgg tgtaacaaat ataatattgg attatgtatt
      721 tattaaatat tttggaatgg gtcttttagg agctgcagtt gcaactgcta taggtattat
      781 tttaacttgt gttttaggta ttatttactt cttatctaat aaatctacac taaaattaag
      841 aaaaccaaaa accgatttta gacttataag agatactatg ataaacggtt cttctgaaat
      901 ggttacagaa ttatctacag gaattacaac attcttattt aatgtagtag ctttaaaatt
      961 agcaggagaa aatggacttg ctgctcttac tatagtattg tatgctcatt ttttaatgac
     1021 atcagtctat ctaggattcg ctgctggagt gtctccatta ataagctata attttggtgc
     1081 tgaaaacagt gataaattaa aagaaacatt taaacattct ctaaaattta tatttgtttc
     1141 ttctctttta gtgtttatta ttgctttagt atttgcacca tttattgtta gggtctttgt
     1201 aagtccagat aacacagtat ttaaactagc cttacaagga ttaaaaatat ttgcatttgc
     1261 ttttttgttt gttggtataa atatatttgc atcaggattt tttacagcat ttcacaatgg
     1321 aaaaatttca gctattatat cttttagtcg tgcctttgtt tttataatca taggaatcat
     1381 aattcttcct cctatgttaa acatgactgg attatggctt acagttccat ttgctgaggt
     1441 tataaccata tttatatcta ttctatttat aaaaaaatat aaaggtagat ataagtatta
     1501 aaaattaata cttaaaatca acatttatta aaactaataa acctgcttta aaatacaaat
     1561 ttacaaattt gatttttaag caggttttat tttagttata ataagtcatt tgcatactct
     1621 ttaattggtg cttttctaag taataaaatt ataaatatta tgcaaataac tataattcct
     1681 acctttgttc caaataaaat aaaaggattc tgaaatgaat aaataatttt ttctaaatac
     1741 aatagcattt tatgcatatt tttagttgca aaattaacta tataaattac cattaatatc
     1801 ggtaatccta tcaatatagt aatactagta acttttctaa ctctatatgt aaaagctcca
     1861 agtaacactc ctataaaacc tatacatatt tcatttatac atatttgtac aaaataattt
     1921 aaataagtta gtggcatact tgatgttgaa ggatctatca gtgctgatat agatgtccaa
     1981 atcatatctc tctttagtaa aactgatagt tctatactgt aacctgttat aatttctata
     2041 aataacttcg ataaaaaaat caaaactata cctaaaatag aaatcacaaa gcttaaaata
     2101 attaaatcta tgacaatagc ttttatacaa ctttttctat ctgctcttat acttaaagct
     2161 cctgaaaagt ctttatttgc tatatttact ccatacacaa atagcagtat agttatatat
     2221 gttaaaatag gattcacaat cgtggataaa ttagttttag atgcttctcc aaaaatcttg
     2281 agaataaaca ctggcaaaat attcataaca attcccacaa tcataaatat aattatatac
     2341 aatttagtat ttttattaaa aaattttata tatggcttta attcattcaa ttacaattcc
     2401 tccttcttag tcatgttaac aaacatatct tgtagcccta tgtatcctac atcaatattt
     2461 gaatttttaa gtattttttc atcattttca ctaaaatcac catagtaaaa ataagcaact
     2521 gcatttccaa atgccttttt aggttttata ttttttatac attctaattt ttctaactct
     2581 tctttatttc cagtaatata atgagctttt tgttttatga catcaatttc ttcatctata
     2641 ataatttttc cctcatttaa tattattaca tgctccagta aatcatctat ttcatcaatc
     2701 aaatgagttg atattattat tgtccttgga ttttttatat aactatctaa aattatgtta
     2761 taaaactctt gtctatttac tgcatccaaa ccaattgttg gctcatcaaa tatggtgata
     2821 gctgaattag aacaaatacc tattatattt gaaagtatag ttttcatacc tcttgacagt
     2881 tgtttatact tttttttagt atttaaatca aaatatttgc ataattcttt ttctaagcct
     2941 ttatcataac ttttataaaa actagaagaa aattcaaata tctgacctac tttaaaatct
     3001 gaactataaa attctttttc cctgacaata caaacttctt ccaaaacttt tatatcttct
     3061 tttgggttct ttcctaatat ttctatctca ccttcatttt gaattaactg atttaccatt
     3121 atatttaata atgttgtttt tccagctcca ttcttaccta ataaaccata tatcttattt
     3181 ttttcaaagt ttagagacat attattaaaa atattttgct tcttaaaact gtgagataaa
     3241 ttttctatct taatagaact actcatatat taatcctcct tgaaattaat tataatatca
     3301 accaattctt ctctatttat ttctaatttg ttagcttctt gaagtaggtt ttttacaaaa
     3361 ttatgtttaa aattttcttt tctagcttct tttataatag tttttgcacc atctgataca
     3421 aacatcccta tccccctctt cttatataaa atatttttat caaccaatat atttatacct
     3481 tttaatactg tagctggatt aattttataa agctttgaaa gctctgttgt tgatggaacc
     3541 tgttcttctt cttttattcc atttgatagt atctcatctt caattgctcg tgctatctga
     3601 ataaatatag gttcttcatt atttaatact aatttcatag gttcctcctt tctccattag
     3661 ttagttactc aactaattaa ctatataact tataatatct tgaaaaatat tttctgtcaa
     3721 tactaaataa aaaacagagg gaaaattccc tctgtttttt gttctttata tttataatta
     3781 aattttaatt tttgtaatcc tatcccataa gttaaaaact ttaataatct gttgtatctt
     3841 cattttttaa tattttacat attgagctag ttcctaacct ttctgctcct gcctctataa
     3901 atttttctgc atcagaaatt gatttaactc cccctgcagc ttttattttt acattttttc
     3961 caacatgctc tttcataagc tttatatctt ctaaagtagc tccacctgtt cccattccag
     4021 tagatgtttt tataaaatct gaacctgaca ttgttactat ttcacacatt tttatttttt
     4081 catcttcatc taaataacat gtctctataa tgacttttaa tactttatct cctatagctt
     4141 tttttatttc ttttatttct tttcctatat tatcataatc tttatttttt atatctgaaa
     4201 tatttacaac catatcaact tcatctgctc cattttcaat agcatctttt gcttcaaata
     4261 cttttgtagc agtagtttga tatcccaaag ggaaccctat cactgtacat atctttattt
     4321 taccttttag atattctgat gctcttttca catatgatgg tggtatgcaa actgatgcta
     4381 cactcatatc tacagcttca tcacataaaa ttttaatatc ttcccatgta gttgtagctt
     4441 ttaaaatagt atggtctact gtttttaata tatgtttcat aaaatcctcc taaatttata
     4501 attaatataa ttagattact tttttaaatg caattttctc tttgcaatgt ttagtatcat
     4561 atcaacctct tcaactttaa aaataatcat tagtagtgta taaattccac ctccaactat
     4621 aactgaaata gctagtgata caaactcatt aaatgttcct actcccaaaa ttccaaatat
     4681 aaatttatac gtaaagtatg ataaaacacc cataattaaa gatgcaacta ccgatttcaa
     4741 agttgctttt attattttat cttgtccaaa atatccaact tttctcttta ggttcaagaa
     4801 gagtaacaat atacatgcaa ttgatgatga actagttgca agagcaagac caccatgagc
     4861 cattggtttt attaaaacta agtctaacac tatatttacc cctacagata taatcccatt
     4921 taccataggt gtttttgtgt cttgtaatga ataaaaaacc tttcccaaaa tatctcttaa
     4981 tccaaatgca gtcataccaa ctgcataaaa aattaaggca gttgctgtca tttgggttgc
     5041 cctcgcatca aaagcacctc tctcaaaaat gattctaaca acaggagttg caaaaaaaat
     5101 agaagcaact gaaataggaa tcatagaaat tattatcata tttatactag atttaactga
     5161 actagtaaac ttcttttgat tattttctgc acttaactta gataacatag gatacataac
     5221 agatactatt gaagctgtaa atgtccctgt aacaaaacca tttaatctat ctgcataagt
     5281 aagtgctgga atgcttcctt taactaatgt tgaggctaaa gttgtatcta ccaatgagtt
     5341 cacttgatta acagcaacac ctataaatac tggtgaaagt aatgctagta atttaataag
     5401 gctatcatct ttaaagttta agtagtataa gtatttgtag ttcgtttttt taacaaaaaa
     5461 catgtaaaag agcagttgaa ctaccatagc aaccacagca ccaattggaa gtatgtatgg
     5521 tccaaatatg gtacttaaca ttattgaaat aattattact atattatagg gtatacttcc
     5581 aaatccaact acaataaagt tctctttaat ttgtaaaaat gctgacataa cacttgtaat
     5641 acctatgaat attattcctg ttctgaacct cccatgacta aagtcacagg gttcctgctt
     5701 catagaattt tgcatactaa aatatgtctt actaattctc cacaggctat ccccgtagtt
     5761 cctacggttc ttacataagt tatttatttt gaatcgttat tagtcttaat ccttctttta
     5821 atatatttat acttgcattt atatctctat cgtgagtttc attacaacta ggacaaatcc
     5881 attctcttat atttaagttc ttaacttcct cgtttttata cccacattta ttacatattt
     5941 gtgaacttgc aaagaattta tctaccttta caatttgtct accatgccaa ttagccttgt
     6001 attctaattg acgaataaac tctgaccatg atacatcaga aattaagcga gatagtttac
     6061 gatttctaat aatattcttt acttgtaaat c
//
LOCUS       DAMJYC010000170         6056 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJYC010000170 DAMJYC010000000
VERSION     DAMJYC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.177"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            37..1518
                     /locus_tag="PC472_003808"
     CDS             37..1518
                     /locus_tag="PC472_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2729081.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFIMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1551..4988
                     /locus_tag="PC472_003809"
     CDS             1551..4988
                     /locus_tag="PC472_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2729082.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5006..5752
                     /locus_tag="PC472_003810"
     CDS             5006..5752
                     /locus_tag="PC472_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2729083.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5701..6009)
                     /locus_tag="PC472_003811"
     CDS             complement(5701..6009)
                     /locus_tag="PC472_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2729084.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2515 a          599 c          931 g         2011 t
ORIGIN      
        1 atattataat tgctaaagag agtgaggtgt agctatttga aaaaaaatag attaatagca
       61 aagaatatat caatagcatt tatagtacta ttctttttta gtgtatttac attcttttat
      121 gtaggtaata ttaatagagt gttagaatat gaaacaaatg acataataac agttacaatt
      181 gctggatgga ttatactttc atttttattt ttaggtatta taatttacat attatattca
      241 aaagcaaata gtcaaaaaac tatagaaaaa gtagcatata cggattttgt aacaggttat
      301 tccaactgga gaaagtttga attagatgtt acaaatttat taaagaagac atcacaaaat
      361 aataaatatg caatggtcat attcgatatt gataaattta aagcaattaa tgatatatat
      421 ggccataaaa agggaaattt aatattaaaa gatattgcag atactttaaa tgaattgact
      481 aatatcaatg aaacttttgc tcgtgtaagt gctgataatt tcaatatatt attaacatat
      541 aacaaaaaag aggatataat aaatattatc aaaaagatta tggcaaataa tgaacttgta
      601 aacttatctt ttggaattta tgaaatcaaa gataaggatt tatctgtaag tgtatatagt
      661 gatagagctt cacttgcaaa atcatcaata aaaaataata gtgatgtaaa ttttgcattt
      721 tttaatgata aattaagaga gaaattatta cttgaagata agatagaaaa agaaatggaa
      781 tatgctcttg aaagtgggca attcataatg tatttacaac caaaatacaa tattaaatta
      841 gataaatttt gtggaagtga agccctagtc agatggcaat atacagaaaa agaggtcata
      901 tatccaggtg attttattcc tatatttgaa aaaaatggat ttattagaaa gattgatatg
      961 tacatattag aacaagcttg taaagaaatc cggtctttat ttgataaagg aatttcacct
     1021 ttacctatat ctgtaaactt ctcaagagtt gattttttta aaaaagattt tattgaaaac
     1081 atagtaaata tatgtgatag atataaaata ccatatagtt taatagaaat tgaaattact
     1141 gaatctagta tgtttggaga tactgatact ctatttaatg ttagtagaaa tctacaggat
     1201 attggcttta tagttgcaat ggatgatttt ggctcgggat attcttctgt aaatatgctt
     1261 aaaaatatac cattaaatgt aataaaatta gatagaggat tttttgttga tgataaagat
     1321 gttgataaga gtcaaattgt tataaaaagc atagtttctc tcattaagca attaggaata
     1381 agggttgttg ctgaaggaat tgaaacaaga agtcaaatag aaatgttaaa aaaagcaaat
     1441 tgtgatattg ttcaaggata ttatttttca aaaccattac caattaaaga atttgaaaaa
     1501 ttagtctata aaatatagtc agcgagtctt aaatgggagg tggtgattaa gtgataagag
     1561 caaaatcgtt aaaaaatgta ctgagtattg ttttaatatt tttatttaca ataatttctt
     1621 tttctgattt tgcatttaaa actaatgtcc aggaagaaga aaatagtaaa attaatttaa
     1681 gggaaatatc cctacaaagt tccaatgcta taaaaataaa attagatagt tctctatcct
     1741 ttattaaaag tgtggcaaat atggtttctt attttaatac tatagataac tcaaaaatag
     1801 ttccattatt agctaatata tcaaaagaat ctgactttca aagaatctct attgtatctc
     1861 ctgatggaat aagtcatacc agcgatggca aaattatcaa tgtgtcaaag tatcagtatt
     1921 ttaagaaagc tattaaagga gaaagtaata tatctgaagt tgtgaaatct ccaattgatg
     1981 gaaaagatac ttttgttata gctactcctg tatttaataa gaataataaa ataattggtg
     2041 ttttatacgg ttcttactac aatgataaat tagctgaatt tatagatata acaagtttta
     2101 atagtgaagg gtacatagat atatttgaat ctagtggtca atttgtttta aaatcttcac
     2161 atacaaacaa actgattaag tcaagtagta acatatatga tatatttaaa aaagcttctt
     2221 ttgatgatgg ttatagctat aaaaaaatat atgaagatac aaaaaataac aagagtggtt
     2281 ttacgtcata tgaatacaaa ggagaaaatc gttatgctac ttatacaccc attggtgtaa
     2341 atgactggta tgtaatttct gtgataccac aatatactgt aagaagaaac attgataaaa
     2401 taaatcaatt agcattttct cttattttca aggtattctt attgtttatt atgcttataa
     2461 tatatattat ttataaaaac aataaatctc agaaagatat actaaaagct aacaaagaaa
     2521 tctcatctat tacaaatagc attcctggag gagtacaaaa gtgtaccctg ggtgaaacat
     2581 gcaatattaa ttttttaagt gatggattta tagagttgtt tggttataca agagaagaaa
     2641 taaaaacatt gtttaataat gatttttata gtttaataca taaagatgat gttgaaagaa
     2701 taaaaaaaga aatttttaaa caactttaca aaaataaaac tgttgaatta gaatatagga
     2761 ttataacaaa aaatggtaat acagtatggg ttttagataa aggggagctt ataacagaac
     2821 ctgatggaaa agaatatttt tgttgtgtaa ctgtagatat tactagaaca aaacaattag
     2881 aacaagagat ggaacaaaaa aatctagaac ttgaaatgat atcagaaaat gttttaggtg
     2941 gtataattat aacagaattt aatgatgatt ttactataaa atacgctaac tctggttatc
     3001 tttcaatgat tggatataca cgtgaacaat tagaaaatga acttaaatac aaagcttgta
     3061 atcttgtttt ttatgaagat agaaagaagg ctcttgaaag tatacaacat caattaaaca
     3121 caaaaggaat agctgtatat caacatagaa taagaagaag agatggtaat attatttggg
     3181 tttctataaa aggaaggctt attgttgatg atgatggtat tgaaaaaggt atttggattc
     3241 taaatgatat aacagattta aaaaatgcag aacaaaaact taaaataaat gaagaaagat
     3301 ttagaattgc attaaagcaa acatcaaaca ctatatttga gtatgatata aaaacaaagt
     3361 cacttttaaa tactagtaaa gagcttgata aatataatat ttataaaatt actaaaaacg
     3421 ttccatattc tttagtagaa tcaggagtta ttcataaaga ttttagtgat gattttataa
     3481 acatgtatga aaatgtaata aatggagcaa aaaaagcaag ttgtgtagtt aagatgaaac
     3541 ttccagatga aaattatata tggaataaag ttaccttgac tactattttt gatgaagatg
     3601 gtaatcctgt aagagcaatt ggtatacagg aagatataag caaacaaaag gaagcagaga
     3661 taagatacaa tcaagaaggt aagtatcatt cagctatatt gtcggaagca atgataacct
     3721 atgaaataaa tgtatcaaag gatactttta tcaaaggaaa tgaaagttgg caaaaaccat
     3781 ttggaattaa gcctgacaat aactattctc aaatggtaaa aactatagca aatagtattg
     3841 tacataaaga caataaagaa acatttttaa atacattttt atgtgaaaac ttattggatg
     3901 catataaaaa taatgtgact gaaataaaat tagaatatcg aagaatgaat caagaaaata
     3961 aaatgatttg ggtcatttgt acaatgcact taataaaaga ttctattacg aacgatataa
     4021 aagcacttgc ttatgtcaaa gatattgaca agcaaaaaaa tgaagaaatt agattaaaat
     4081 taaaagctga aagagatact cttacaggat tatataataa aggtactaca gaaaaattga
     4141 ttaatgattt cttgaactct gaggaagcaa gcttaggaat gcatgcattt ttaattgttg
     4201 atatagataa ttttaaatct gtgaatgata atttaggaca tgtatttgga gataaagtat
     4261 tatttgaagt ttctaaaaac ctaaaaccta tatttagaaa agatgatata gtaggtagaa
     4321 ttggtggaga tgaatttata gtattcttaa aaaatattca gtcttttgag aatatcaaaa
     4381 agaaagcaga aaaaatatgc gatatattta gaaattctta tacagaaaat aaaaagacat
     4441 ataaaatatc tggaacagtt ggaatttcat ttgtaccaga acatggaact gtatttgaag
     4501 aactttataa aaaagcagac atagcacttt attatgcaaa gagcaaagga aaagataagt
     4561 atgcacttta taatgattgt atctaccaaa atgaacttga aaataatata agtgataata
     4621 atataaaatt agcagataaa tgtgagaaat ccaaaccagt acttaaagaa attcttgatt
     4681 ctattgataa ttatatctat atagtgaatc caaatactta tgaacttctt tttgtcaata
     4741 ataaggtgtc tcgtttggat ttagagataa aattaggaga aacctgttat aaatcactat
     4801 taaaatctga tagaccatgt aataattgcc ctcttaacgg attagaagaa aataagcatt
     4861 caaaattcaa aatagagaca aatacatttg gtaaaaatat cagtatagca agttggatta
     4921 attgcataga tggagaatac aactgtctta tagagtgtat agattcttct ttatacaaaa
     4981 ataaataaaa aaggactatg aagatatgtt aaatagtata actttgatac ttggtataat
     5041 atttacaatt tactattttt atctcaaaat agtttttgga ggaatttcat ttagtgagat
     5101 tttcttagtt gtaggaattg tactaattat ctatcaaata tttaagaaaa aaattaaaga
     5161 aaagaaggtt ttttataggg tgttaaaaat aataatcagc atttttttaa tagtatttgt
     5221 agttactgag agtctaatta ttttttatcc caaaaatagt ttagaatcaa aaagtgatta
     5281 tctcttaatt ttaggagctt ctgtaaaaaa gactacacca agtacaaccc tgaagggtag
     5341 gttagacact gcattgaaat acctaaaaat taatgataat tgttatgtgg tagtatctgg
     5401 tggtaaagga agtggagaaa aaataactga ggcaaaagct atgaaagatt atcttataaa
     5461 aaatggtata gataaaaata ggataataga agaggataaa tccacgaata cttatgagaa
     5521 ttttaaatat tcaaaattga agatagaaga acatagtcaa ggaaaactaa gcgatttaaa
     5581 agtaaaaata gtaacaacag attttcatgt tctcagaagt aaaatattag catatagaaa
     5641 tggatataaa aatacgtctt tttatgctag taaatcaaaa ctttcatttg taccaactta
     5701 ctatacaaga gaattttttg cattatggaa aacaatcatt tttgatagat aataaataaa
     5761 aagactagat ataaactcat atctagtctt ttctattccc ttaattctaa tccattttcc
     5821 aactttactt gttgtagaaa tttcaacatt gggatttaca agtaattgct tataagaagt
     5881 tttatatttt acgattccaa aatataattt tccttcataa tccattataa atccgaatgg
     5941 tcttactctt ggtttattat cttcaactgt agcaaaatag aatgttgagt tttctttaaa
     6001 aaaattcatt attttttcat gattaaatcc tctttgtatc ttatactaat ataatg
//
LOCUS       DAMJYC010000171         5995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJYC010000171 DAMJYC010000000
VERSION     DAMJYC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.178"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(964..1515)
                     /locus_tag="PC472_003812"
     CDS             complement(964..1515)
                     /locus_tag="PC472_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729085.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1706..2668
                     /locus_tag="PC472_003813"
     CDS             1706..2668
                     /locus_tag="PC472_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2729086.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2963..4807
                     /locus_tag="PC472_003814"
     CDS             2963..4807
                     /locus_tag="PC472_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2729087.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYYSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5129..5713
                     /locus_tag="PC472_003815"
     CDS             5129..5713
                     /locus_tag="PC472_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2729088.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2344 a          585 c          925 g         2141 t
ORIGIN      
        1 aaggagatgc cctttgaaaa taaaaattaa aaaatattta atgctattac aataggaact
       61 agaactacac ttaataaata tacagaaatt agatttggct caatataaaa tacaaatata
      121 gaatttaggt gttttttatg aaaaaatttt tatatgcttt atatagtttc attgttatta
      181 tagctaattt tagattaaaa gaaaaaaatt ataactttat tttattagca atctttttta
      241 tattgctgtt aattcaaaat ggagttttaa aaacaggtta tcaaaataga gctacatttt
      301 atcaagataa ttattttaat agagaggata aaaatgataa gataaaattt atgactataa
      361 agatttttat atttttgtca ttaccattat gttttaatat actattcaat tatttaacct
      421 cttaaattat atttagatat aggagtctta tgataaaagt aagcttatgt gttatttgta
      481 gtaaatataa agtgcatata gtgataaaac aaaagtttta tataaatgaa gaaaaaaata
      541 taaaataatt tatttaacta cttaaaaata aactaaaagg gataataaaa tagcttttag
      601 gttatggtaa taaaggtatt taaaaagagt gcataagcac tccaattgtg agaaatacat
      661 acacaaatca ttattacaac aatgaaaaat gtacatataa gagacgtttt gtatttgttt
      721 taagtgataa tattttaata ctaactaaat aattatcaca atagtaaaaa agaaaaagga
      781 gtattttgta ctccatttgt caaaaatata aaacttttat ataagcttat tataacataa
      841 ttgataaaag tgtaaagata tataatgaat ttataaagaa aaataaaata aaaaagataa
      901 atcctaatca aaggatttat ctttttgtat agaaaataat tattctatta ttaaatggca
      961 tgactaaact ggataataat ttattattac tgctttcaat gttttttctc cttgattatg
     1021 gtatgtatga attgattcag ctgaaaaaca aatagagtca ttttctctaa gttgataaat
     1081 agagtcattt acttctaatt ttaattgacc ttctataatc ataatatatt cctgtgattt
     1141 ttcagaatga ccaacagatg tataactatg accttcttct aattccattt gaaataattc
     1201 gaagtttcta gttggtgtat tgggataata acaataaaga cgataatctt tattttctga
     1261 aacttgtaca tctatatcag tttttgatac aataaaagtt tcattttgag gttgttccag
     1321 tattgcagta tatggaacat ttagtccact tgcaattttc caaattgtgt ttactgttgg
     1381 gtttgagtct cccttttcaa tttgcgaaag cattacttta cttacgccag ataattcagc
     1441 aagttgacct agacttaagt tacgttcatt acgtaatcta tttaaatttt tagcaataac
     1501 agtattaata ctcatagtaa ctccttaaaa tgtaaattat tgacaaatat aatttacgaa
     1561 tgtataattt atttaacatt atttataatt tacaaatgta taatataatg aacgttaaat
     1621 atactgtaca tccgtaatat aaattatact gcataaagag ttgtttttca acggatgata
     1681 gttgagagat taagggggaa ttgtaatgcg tgtacttaat aatgttacag aggctatagg
     1741 caatactcat atgttacatt tatccaaaat agcagatgaa tttggagttc aaggtaatat
     1801 ttatgcaaag atggaatact taaatccggg atttagtaaa aaggaccgtg tagcattaca
     1861 aatgattacc gaagcagaaa atgcaggttt attagttcca ggtcaagcag ttgtggaatt
     1921 aacaagtggt aatactggaa caggactagc aatagcttgt gcatgtaaag gatataaatt
     1981 tatagcgtgt atgtcaaaag gaaattctat ggaaagagca agaatgatga aagctttagg
     2041 tgctgaagta gttcttgtag accaaatgcc taattcagtt catggacagg tatctggtga
     2101 agatttggca cttgtagaaa ttgaggctca aaaaatagca aaagaaagaa atgcatttag
     2161 agcagaccaa tttaatttag aagcttgtaa tcatgcacat gaaaagtata cagcagaaga
     2221 aatgtgggaa caattggatg gaaatattga tgtatttgta gattttcttg gaagtggtag
     2281 tacttttgca ggatgttcaa aaacacttaa aaaacataat tcttctatca aatgttatgt
     2341 tgttgaacct gaaacagcag caatttattc aggtaaagaa ataactgacc aagggcataa
     2401 aatacaaggt ggaggatatt ctatggattt gccattagta gacaagaaac ttattgatgg
     2461 ttatattcaa gttacagacg aggaggcaac tgatgtagca agaaaattag ctaaattaga
     2521 aggtgtattt gcaggatttt cttcaggggc aaatgtatgt gcagcaatta agttattaaa
     2581 gagcagtgaa aaaggaaaaa atatagtttt aactcttaat gatagtggtt taaaatattt
     2641 aagtactgat ttatatgaag aattgtaata atagcacaat agttatgcaa tgttttttaa
     2701 attaagtata tagatttaaa ttataaaaag tatctcaatg taatttttga aagttataaa
     2761 ggagatactt ttttattttg taaatatgga gtatcctact tataagtatt aagctatata
     2821 gatattgatg attagctatg caataaaaaa gagtatgtgt ttttgcattt atacatcata
     2881 acttggaata atacatatta atgttctaaa tgtatttaaa acatatgata aaattagaac
     2941 aaatagatac aaggagataa aaatgcaaaa gagaatttta agaaatttta tatttacggg
     3001 taaaaatatt ttaagtaaac ttgtttttat aataaaatat aattttagta cattaatttt
     3061 atttgaatta tttcataaag gattagcctt attttttata tggccatcta ttagatatat
     3121 atttgatatt ttaataaaaa gtttacatat agtctaccta aatatggata attttataaa
     3181 agttttgacc aatcccatgt caatagttct gataatacta tcagttataa tactagcttt
     3241 ttatgcattt tttgaattta cctctgtaat tatttgtttt aataaatcga taaaatatga
     3301 aaaaataggg ttatttgaac tctttaaaat atcatttgag aactctataa aattgttata
     3361 tcctaaaaat atactattat ttatatttgt gatattaata attccattag taaatacagt
     3421 tttaatttca gggtttatag gaaaaataca aataccagag tacatactag attatataaa
     3481 gtctgatata attttaaata taatctatat gatttttctt ttaataatat atatttcggt
     3541 tattagatgg atttttagca ttcatgagat aactttaaat acagaaaatt ttaaaagagc
     3601 aagaaaagaa agtgttaatc ttataaaggg aaagattata agaacaatta tttattcatt
     3661 aatattatct ttagtagtag ctattattgg atatgcgatt tattatagtg gaattatatt
     3721 tataggcata tggactaaat attgtactga tatggaattt ttgaagaatg tatttataaa
     3781 tagatgtatt gcatttgaag aatattttag attgattttg tcaatattca tttttatatt
     3841 ggacattgga tttatctcag caacttatta tcaatataaa ggagtaagta tttctaaaaa
     3901 taatattaaa aaagagaaaa aatctatatc aaaaattgtt tttaatttag tattaatatt
     3961 attaatttct tatatagagt cagttgcatt ttcttcacat ataaatggta tgtttaatac
     4021 gtcattttca ttttatacaa cagcaattgc tcatagagca ggagctgaaa tggctccaga
     4081 gaatactttg gcagcaatag aagaagcttc acatgctaaa gctgattatg cagaaataga
     4141 tgttcaagaa actaaagatg gagaacttat tgtacttcat gattcaaact ttaagagaac
     4201 tacaggagta gataaaaatg tttgggatgt aaattataat gaagtaaaga catatgatgc
     4261 gggaagcttt tattattatg gaaaaggtta ttatgatgaa aagtttgttg gggaaaagat
     4321 accaacactc aaagaaatga taaaatattc taaaggtaag gtgaaattat taatagaaat
     4381 aaaacttaat ggacatgaaa agggagacgt agtaaaaaaa gttgttcagt taattaaaga
     4441 aaataatttt gaaaatcaat gtatagttgc atctatggat aaaactatat tacaaaaagt
     4501 aaaaaaactg gatgagaatt tactgacatg ctattttatg gcaattgcat atggtgattt
     4561 ttcaaagtta aattatgttg atatatatgg aattgaatca acttttgtta ataaaacagt
     4621 agtagaaaag attcataaaa tgaacaaaaa aatcttcgtt tggactgtta atagtgacag
     4681 tttaattgag aaaatgcttg acctaaatgt agatagtatt attacagata atccatacct
     4741 tatagagagt gcaatttatt ggaagaaaaa tggatttatt agtcaagtgt cagattattt
     4801 attttaaata ttctataatt gtattatcca gtaaaagaga tattattaaa atcaataata
     4861 tctcttttta acataaataa ggttgttaaa ttaaggttat tcttataaaa attgtgctta
     4921 tcagctataa acaaggaata aaagtgaaaa aattgatctg agattattac agttaaaaga
     4981 gttttaagat gacagagaga tagattgtca actgtatatt taataaatct tgttatttat
     5041 taaattttat ataagtaaaa tatataaatt aataaatctt atgctagaat atatatgaaa
     5101 aacaatataa atttggagga ttaaaaacat gacagagcga gaaaaaatgt tggcaggaga
     5161 actttacgat tgtggtgata ttgagttgtt aactcaatgg cataaagcaa aagacctagt
     5221 aagagattat aaccaaacag attcctcgga tttggaaaaa aaagaaaaaa tattaaatga
     5281 gttacttggt ggtaagggta aaaacttatg gattactgca ccgtattatg tagattatgg
     5341 aaataatatt tattttggaa ataattgtga agtcaatatg aattgtacgt ttttagatga
     5401 taataaaatt atcattgggg ataatgcact aatcgcacca aacgtacaaa tatatactgc
     5461 atttcatcca actaatgctg gtgatagatt tggtaatgcc aaagaagatg gttcatttga
     5521 attttgtaaa actcaaacag ctccagttac tattggagat aatgtttgga ttggcggggg
     5581 tgctattatc atgccaggtg ttactattgg agataatgta gttataggtg caggaagtat
     5641 tgttacaaaa gatattccaa gtaatatgat tgcatacgga aatccatgcc gtattattcg
     5701 agaaaataaa taaagttaaa ttttcttgca aaatctcaaa aaacaccttt cttactttat
     5761 acatagggta ttttctaata ctttcacatc catattcatt tgttaaattt ttgtggaatg
     5821 tagtttttat cacttctatc ttacatttcc accatttttt ataaaagtca ttcctttata
     5881 gatttattta taatagtata tattctattt gatgtttgaa taagcactta tttttaagta
     5941 aaatactaac ttttaggtta gtattttgct atagtatgat gattaagtaa gaata
//
LOCUS       DAMJYC010000172         5893 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJYC010000172 DAMJYC010000000
VERSION     DAMJYC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.179"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            167..2191
                     /locus_tag="PC472_003816"
     CDS             167..2191
                     /locus_tag="PC472_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2729089.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKLLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(2421..3608)
                     /locus_tag="PC472_003817"
     CDS             complement(2421..3608)
                     /locus_tag="PC472_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2729090.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3810..5081)
                     /gene="brnQ"
                     /locus_tag="PC472_003818"
     CDS             complement(3810..5081)
                     /gene="brnQ"
                     /locus_tag="PC472_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2729091.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(5222..5875)
                     /locus_tag="PC472_003819"
     CDS             complement(5222..5875)
                     /locus_tag="PC472_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2729092.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
BASE COUNT         2199 a          925 c          709 g         2060 t
ORIGIN      
        1 tatcttgcaa aaataatttc aaaataggtt tttataggat aaaatcctaa aaaaacctat
       61 tatttaatca aatatataaa tttatactac tatttgttgt ataatataac ttgcattata
      121 ctttaaatac tttaaaaata aatactattt tgttaggaga aataaaatgg ataaaaagaa
      181 gttaatcctt ataacatatg ataaattgaa tagtgaccac tacaaagaag aattaacaaa
      241 cttttttgga gacgaaataa tcatagaaac acaaaatata cttgatggaa taaaagaaaa
      301 tttagaggga gatgtagtgc tttctctatc acctttaact agtaattttt taataaaaca
      361 ttttaaggaa gatattgaaa ttattcatgg aacgaaagca ctttcaaaac ttggatatga
      421 aaaaatgatg aaactgcctc caggtacaaa attgttactt atgacaacca ataaaacttc
      481 agcatttgag atggctactt atctatataa aattggtata aatcatatag attttgtacc
      541 gacatatcca gattgtgatg aaatatatga tttagataca gctataacac caggtcagat
      601 tagatttata ccaaaataca taaaaaatat tgttgattta ggttggagaa aaatctcatt
      661 agatacatat atgtcattat tagtagtatt aaaactaaaa aatgagaaat ttatagaaaa
      721 attatacaaa ttgtccaagg agacactaag tcatgatttc ttaaatacat cattagataa
      781 tatatctaaa ttaaaaacta tactttacat gaccattgat gaaataggtg atggtctaat
      841 atttttcaat acctttaata aggttacttt tgtaaataag tctttactta atatgttgga
      901 actagatgaa aaacttataa aatcaccttc cttaatggaa tatatgccaa agagtttctt
      961 agataaaatt acaaagaact taaatataga taatatgatt atatacatag acgaaataga
     1021 taaaaaattc atactttcta aaaaaccatt ttatctttat aaaaatatag agggttgctt
     1081 aattacatta aaagatgtaa acaatataga aatattagaa caaaaaataa gaagtgattc
     1141 tgtcaaaaga ggttatgtag ctaaatataa atttaataat attattggaa atagttctat
     1201 tataaaagat tgtattaaaa aggcaaaaaa aatggctctt acagataatc ctatattaat
     1261 aacaggtgaa acaggaacag gtaaagaagc ctttactcag tcaatacaca atcattcaaa
     1321 tagaaaaaat aaaccttttg tagcaataaa ttgtgcttca ttaccaagtg aattacttga
     1381 aagtgaacta tttggatatg aagatggttc ttttactgga gcaaaaaaag gtggaaaaaa
     1441 aggattattc gaattagctc atactggcac tatattttta gatgagatag gagatatgcc
     1501 acatgattta caggttaaac ttttaagagt cttacaagaa aaagaaatta gaaaaattgg
     1561 tggtacaagt attattccaa ttgatgtgag aatattagct gccacaaata aagatttaga
     1621 gaaattaatt atagaaaata aatttaggat ggatttattt tataggataa gtatgttcac
     1681 tttagattta cctcctctta gaaagagatt agaagatata cctcttcttt tagaatcatt
     1741 tttaaaagaa cttccctata aaaacataaa actcgataag agtcttttag aagctttaaa
     1801 ttcatacact tggatgggga atattagaga actgagaaat tgtgttgaat atatggctta
     1861 catgggttcc aattatctta caataaatga tttacctcaa aacatatctt caaaattaaa
     1921 taataatcat atgtcaagta atatgtccat attcaatgat ttaaaccaat atgacaaaaa
     1981 catatgcatt tctatcctaa aatcattgca catgaaacca atggggagga caaagttaat
     2041 gaaatttatg gaatataatg tcacagaata cgaagtaaga aatatgctag aatatttgac
     2101 acgcaatgga tacttaattt ctagtaaggg aagaaaaggc tcatcaataa ctgagaaagg
     2161 cataaaaata atagaaaata atataattta aacctatagt aattcatctt tactttatca
     2221 aaatttatat ataattataa ttttttattt ttctataaat tttttataaa ataataaaac
     2281 ttatttatat taaaaacgaa gctatctcaa catatggata agcttctttt taattgttaa
     2341 aaagataaaa atatatttga tttaacagaa aaagagtgcc tcataaacta aaaagttcat
     2401 tttgagacac gtccttaata ttaataaatt aattggctta aaattactga ttgataatta
     2461 taaagtacct ctgttaaagg agtaaattct gaaccgattc caaatcttaa ttctgtaaac
     2521 gcctctaaat aatctgggtc acatacatct tcttgtgtta aatcttgttt caccatttca
     2581 gtagcaagat aaattgctac tgatggtaga aaagcatcca ttggcatagg atatccacca
     2641 agtctatttg acatgccaaa agccttagcc ttttcactta tcatgtcgtt tattttatca
     2701 taattttgag catctttttc agatattttt agacccataa cactaggata tgtttgtatt
     2761 ggtgatggat tgctttgctc tgcaactata tactttaatt ctaaagcctt agtcaatata
     2821 acttcatcca tatactcatt tacaccaaat acatttatat cttttccata ttttttaact
     2881 tgtttttcaa tatcttcatt taaaaactgc ttaactttgt ttttgtcttc ttcagtattt
     2941 atatttggag tattaacttg tacaaatggc aatcctatat ttttgcaagt ctctttaatc
     3001 atttctaatc ttttggctat attaacatct ttcaaatcat ctgtagaggc ataatgaata
     3061 aaagcttttg ctcccatttc ttttgaacgt tcagctaata cttttcctct ttcctctgca
     3121 ctaaccccta gattcacatc aacaaattgg cttaattggt ttttatcatc tcccataggg
     3181 gcagaaatag ttattatttc tggacgcttt tcctttacct tttgtaaagc aggtaaaagc
     3241 cctgcttggt cagttgaaac tacaatagcc tgaacttctt tatcgtcagc caactttact
     3301 atcttattaa tagcactgtc tatattagaa gtaaaattct ctggcaatac aacatgcttt
     3361 atagtttgtt taactttagt ttgaccatct ttgtctttat caattttatt ctcttcctca
     3421 tattcttttg ccatctcttc caccatattg tattgtactt tattttcaga taatggttga
     3481 gttaccactg caactttaaa atcatctata attttctctt tatctttctt gcctacaagt
     3541 aaactacaac ctgtaagtcc aataattaaa catatggata gcataaatgc tgttattttt
     3601 ctaagcatta attaaaacct cctaaatttg tacattctta tcactattac taattttatc
     3661 atacttcaaa gaaaaattat attataaaaa attatataca tgctattata atccaatcta
     3721 aaaagtacta tgggtagatt aggtttagat taaacctacc catagtacta aaaattaata
     3781 tattataaaa ttaaattaat tacaaattat tatgctactt gtttgttgct ttttataaag
     3841 ttaccaacta ttccacccaa tatagctggt attacccagt taaagcccaa acctgcaagt
     3901 ggcaagcttt gtataaatgg aacagctcct gtcattccat ttatagctga taataaacta
     3961 atcactaatg taacatatgc tgcaccttta aaagcattat cattctttat atgtttacca
     4021 gccaactcca taacaactat tgctaataaa acaggatata tagtttgtag gattggaact
     4081 gatatggcta ttatagaatc aacacctaaa tttgatataa gcatactagc tacacatata
     4141 cctataacca gttgtttata actcgcctta cttattcctt caaaataagt tgatattgat
     4201 gctgttaaac ctgtagaagt agtcagacag gcaagtgcca caattacacc taaaattacc
     4261 tttcctactt gaccatataa caatatacta gttatttcaa ctaataaaga tgcttgaggt
     4321 atactagtat catataaagt tgaaactgtt gcacctaaat aagtaagacc accatatact
     4381 actgctagaa atacacaggc tacaaaacca gctttaatag tcattcctat ttggtcttta
     4441 tctgatgtat atcctttagc tattatagac atcaacatta tagttgccat agatgctgca
     4501 cctagagcgt ccattgtttg ataaccttgt gatatacctt caccaaatag atttttatct
     4561 atcatatgct caggactcat agggcctgct ggagaaatta taccctttac tattaaaaca
     4621 aataatacta ataataacgc tggagttaaa tacttaccta ttatgtctat aaccttagaa
     4681 gacctaatag taaatgctaa agttagccca aagaagatta ttgaaaatac agctttagct
     4741 actggaccat ggtctccaaa tataggcata atacccattt catatgttgt tgcgcaagtt
     4801 cttggtatta caagaagtgc taaacatgct agagcagcta cactcattac tcttgctaac
     4861 cctttaccag ctctcctaag tacatcatcc atgcttccct tacatcttgc tgttgctata
     4921 ataacaagca ttgctatacc agcatctgca acaacaaatc caataagagg aattaaccag
     4981 tctttgccgg attccatccc taaaaatgga ggaaacataa gatttcctgc accaaagaac
     5041 atagaaaata aagcaaaacc aactataaaa atatctctca tctgcttact cacactaaat
     5101 tacccccttt gaaaatattt tgaatattaa gataatctta ctttattttc agaaaaatgt
     5161 caatctggta aaaattcaca cattgttaac gttttcccaa tttattttat gaatattttt
     5221 ttcattttat gataattccc atcaatttcg ttaaatcaat tgcttgtgac acatcaaaaa
     5281 ttgctcctct aatatcatct ataccaatct caattccctc aatattatta gtagtaaaat
     5341 ctatgccaga aagtttagtt ccattaaagt ttgctttttt taaatttgta tactcaaata
     5401 ctagattgtc accttttact tcttgaaata cagaattttc aaaatttgaa tctaagatag
     5461 aaactatctt aaatttagag aatgaaaagt tagaatactt acctaaaaca tccttgaaca
     5521 aaacgttttt aagtatacaa tcatcaaatc taactcctat caatttacta tttttaaatt
     5581 caactctgta tatacttcct ccatcaaaaa atgtatttga taaattgcag ttttcaaata
     5641 ccacatcaag taaatcaatc ttttttaaat tacaattttc aaaagacaca tttctaaata
     5701 tacaagattc aaagctaact cttacagaat ctaaatcact tataacttca ttttctataa
     5761 ttttattaaa tattttttca tcattaaata tatcttcaag tatatcttca agattttcaa
     5821 taatttctaa ttctcctgta aatttaggtt tttgcacttt aataatttta gtcattttcc
     5881 cctccactat aaa
//
LOCUS       DAMJYC010000173         5883 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJYC010000173 DAMJYC010000000
VERSION     DAMJYC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.180"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            119..1483
                     /locus_tag="PC472_003820"
     CDS             119..1483
                     /locus_tag="PC472_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2729093.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1622..2788
                     /locus_tag="PC472_003821"
     CDS             1622..2788
                     /locus_tag="PC472_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2729094.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2905..3687
                     /locus_tag="PC472_003822"
     CDS             2905..3687
                     /locus_tag="PC472_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2729095.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3806..4459)
                     /locus_tag="PC472_003823"
     CDS             complement(3806..4459)
                     /locus_tag="PC472_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2729096.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4483..5847)
                     /locus_tag="PC472_003824"
     CDS             complement(4483..5847)
                     /locus_tag="PC472_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2729097.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2208 a          749 c          903 g         2023 t
ORIGIN      
        1 ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat gttaggataa
       61 atttaaatct ttttctaaaa atgtattagt ataattctta aagaaaggat ttttaacaat
      121 ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta aaacaagaga
      181 tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac aaaatgaaga
      241 aaaaatactt ttagcactaa agaaagattt aaataaatcg gattttgaag gttttatgac
      301 agaggtaggc atgttttata gtgaattaaa ctttgctata aagaatataa gaaaatggag
      361 caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca aagttgttcc
      421 acaaccttat ggtgttactc tcataatgtc accttggaat tatccatttc aattagcact
      481 tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac cttctgaata
      541 ttctgtgtca acatctagtg tagttaaaga catagttgaa gatacttttt caaaagaata
      601 tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg aaagatttga
      661 ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat cagcatcaga
      721 gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca ttttaaaaga
      781 tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa atgcaggaca
      841 aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg agttaataga
      901 gaaaataaaa tattatacta ataagtattt tggggataat gcatgtaata atgagcaatt
      961 tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg ataaagataa
     1021 gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa ctatagtaga
     1081 caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta tatttcccat
     1141 tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc caaatccatt
     1201 ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag aaatgattcc
     1261 agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt acttaccttt
     1321 tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat tcgatacatt
     1381 tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta gatatgctcc
     1441 atacgataat aaattaatta aggttcttaa aaagataatg taggttatat tgtagaataa
     1501 taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta atttggtata
     1561 tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata aggaggaaaa
     1621 aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat ttataatagc
     1681 tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt ctaataattt
     1741 agatgctata tttacaacat tacaaccttt tataatagga tttgctattg catatcttct
     1801 taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga aattaaaaaa
     1861 actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa cagctacatt
     1921 gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta ttgttggact
     1981 agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata aattaatgac
     2041 tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga atgaatttgt
     2101 tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta tgttaaagaa
     2161 tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtgt acttattaat
     2221 tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat ttacagaaaa
     2281 acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg gaagattttt
     2341 aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa tacttacgtt
     2401 agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa atattatccc
     2461 tttctttgga cctttatttg gagctatacc atctacgcta atagtgatgt ttgtatctcc
     2521 tataaaagct ttctggttat tgttgattat attaataata caacagatag atggaaatat
     2581 tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga ttttgttctc
     2641 cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag ttccaatgtt
     2701 tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggaca aaaagggatt
     2761 accgacagat acttccgatt atatgtagct aaaaaatatt atgtataatt aaaaatatat
     2821 aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt tagatataaa
     2881 attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa atctaaatat
     2941 actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa agaacttttt
     3001 agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt atttcatggt
     3061 ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta tgctaaaaat
     3121 aaaaatatag ccctagtaat accaagagta gaaactagtt tttatactga tatggcgtat
     3181 ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag agctatattt
     3241 cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg gggatatgga
     3301 gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg cagttgcaag tttttctggt
     3361 tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc agacattcta
     3421 tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt actttacata
     3481 cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt ttgtggtgat
     3541 agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagattc tctgggtgta
     3601 gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa tttatggata
     3661 aagaaattta tagatataat tggatagtac acttatggta ttaacaggta tactataaaa
     3721 tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt aaaatttcag
     3781 tctctaagaa tttaataaaa ttttattaag ttcttacacg agttattatt ggagtaaaat
     3841 tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg cataaatccg
     3901 tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca gcttgagaag
     3961 atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata gcaagtatac
     4021 caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta acatatcttt
     4081 tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca taagctatat
     4141 ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca gcattcatag
     4201 caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa gactgcattt
     4261 catttcttat atcttcatat attatagctc ctatttcgct tttacttgag aagtaatagc
     4321 ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt gtcttatgat
     4381 aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt gtttctttgc
     4441 ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt tttttgaaaa
     4501 tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa ttctgtcaaa
     4561 tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta taacagcata
     4621 gatagctcct ttttttgtag cacctttcca gcaaagacca attacaaatg gtgcaaatat
     4681 tacacctgta cataatggag ctggccaaaa caataattct aataaactat caaatttaag
     4741 tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat actttagagt
     4801 attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa tatctcttga
     4861 tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg cagccaatat
     4921 agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca tttgaggtaa
     4981 aacctgaaca gatgatatat ttggaaataa actaccacca atagctccaa ttattcctat
     5041 agggaaagca agaaataata gtataaatcc tgcaattaca ctacttttaa ctgcattttt
     5101 agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg agcctaaaac
     5161 taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa agaatggttc
     5221 ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta agcttatata
     5281 agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg atactgcaac
     5341 tccccacatt cctgatgtat aagtatatat aatgataact ataactccta taattacagc
     5401 tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatca attgactacc
     5461 cataatagct agtatagata ttggtgttac aagactttga taagctctta aaaatacact
     5521 atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata agcttcttaa
     5581 ttttcctgcg aaaaatgcat ttataaagca agctaaagct gcacctatag tagaatacat
     5641 tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc accctcctcc
     5701 aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggcagatttc tacctgcgat
     5761 taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc atatgtatat
     5821 tgcacacgta acaattaaaa gtaacatagt cttttctcct ttataaaata ttttaataaa
     5881 ata
//
LOCUS       DAMJYC010000174         5797 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJYC010000174 DAMJYC010000000
VERSION     DAMJYC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.181"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..410)
                     /locus_tag="PC472_003825"
     CDS             complement(<1..410)
                     /locus_tag="PC472_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2729098.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNI"
     gene            complement(558..1349)
                     /locus_tag="PC472_003826"
     CDS             complement(558..1349)
                     /locus_tag="PC472_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2729099.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1385..2041)
                     /locus_tag="PC472_003827"
     CDS             complement(1385..2041)
                     /locus_tag="PC472_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729100.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2034..2999)
                     /locus_tag="PC472_003828"
     CDS             complement(2034..2999)
                     /locus_tag="PC472_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2729101.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3105..3211)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3501..4445)
                     /locus_tag="PC472_003829"
     CDS             complement(3501..4445)
                     /locus_tag="PC472_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2729102.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNVVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            4556..5023
                     /locus_tag="PC472_003830"
     CDS             4556..5023
                     /locus_tag="PC472_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729103.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5193..5792)
                     /locus_tag="PC472_003831"
     CDS             complement(5193..5792)
                     /locus_tag="PC472_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729104.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         1887 a          975 c          620 g         2315 t
ORIGIN      
        1 aaaatatttc ttataaagta atctaatatc acaaaagcac tataaaaaat tagtattggg
       61 cttattctta gtttttttac tttagaaatg gttaagtata taaaagtaga catattaatt
      121 atgcttaagt taagcaatac aatttggaat ttttgaattt ttaaatttac tactcctaaa
      181 atacttacaa aaactaatac aagacaccct actttcaata tatttataaa ttcatctgtt
      241 ttaaagctac ttatataaac tatagaatat ccaaatatcc aaagtaaaat tattctatac
      301 atatttatag aaactgtata caaatttata tttaaaagtg caataaccaa aaataaccca
      361 ttagctagta tatagacctt attcttactt aaagttcctt tagattccat attccccacc
      421 acttcttcat ttttatatag ttttacctat aaatatgtta ccacaattct tccaaaaaat
      481 ttgtcacttt ctagttataa aaaaaaatga gtaaatattt ttaattttac tcattttgta
      541 gattttgtat attcagttta aaaagctgga actatacttc ctttatattc atcttgtata
      601 aattttttaa cttctttact attcatagcc tctgataaag ccttaatctt atcactatct
      661 ttattatttt ctctacaagc tattatattt acatatggag aatcacttga ttctattact
      721 attgcatcct tagtaggatt taagttagct gttaaagcat aattagaatt tattactgct
      781 ccgtcaacat cttttaatac tgttggtaat tgctctgcat tcatttcttt tattactata
      841 tttttaggat ttttagttat atctttttta gttataagct ctccatcttt tacttctatc
      901 aatttatttt ttgctaataa tttaagtgct ctagctccat tagttgcatc atttggcaca
      961 gctataacag ctccatcttt taattcatct aatgacttaa ctttttctga atagaatccc
     1021 attggctcta tatgaacttt agatgtataa gtaagtttat atcctttctc cttcacagtt
     1081 tcctctaaaa atggaatatg ttgaaagaag ttagcatcca aagacccttc atctaaagct
     1141 gtatttggaa gtacataatc atcaaatatt tttacttcta aatcatatcc tttttctttt
     1201 aacaaaggtt ttgcaacttc caatatcttt gcatgtggat ttgatgaagc tcctactagt
     1261 atttttttat cctctttgtt tgaacaacct actgctgata ttgctattgc tgacaccaat
     1321 gctactgata ataatttctt taatttcatt tttactcccc ctctttaaaa tgtttataat
     1381 ttatttattt tagtttctta taagccagat ttcctactcc ctggattatc tgaactaata
     1441 ttataagtgc tattacagta tataccatta ctgctgtatc aaatctttga tatccataaa
     1501 taagtgctat attacctaat cctccagctc ctactgcacc tgccatagct gtataaccaa
     1561 gtatagatat tattgataat gtaattccag atactattga aggcatagct tcctttatca
     1621 taactttaaa tactatttgt ctttttgtag ctccaaaaga ttttgctgct tctatcaatc
     1681 ctttatcaac ttcatttaat gagctttcta taattctagc gataaacggt gctgccccaa
     1741 tagttattgg cactattgca gcagtttccc ctatacttgt tccaacgatt aatctagtta
     1801 ttggaatcat tgcaactata agtatcataa atgggaaact tctaaatata tttactataa
     1861 atcctaatgc actatttatt gtgctatttg gttttaatcc atctggtttt gtaactacta
     1921 atatgattgc taatataaat cctaatattg ttgccactat agttggtact ataaccatat
     1981 acaaagtttg taacaatgca tttggaaata gtgttgttaa aaagtcaatt aaactattca
     2041 tttatcattt cctcccatct tatgccctta ctagttagat attgttttac ttgctctcca
     2101 cttttatctg aaatgtttat aataagtgaa cctaaaatat catctttaaa ttgttctaat
     2161 ttcccaaata ttatagacac atctatattt agttctcttg ccatagtcgt aataatagct
     2221 tcattagaaa tatctttagg gaatagtatc tttatatttg ttccttttgg caatattata
     2281 ctttcttcac ctattaattt tctaagtcct ttagtatttc ttaagaatat ttcttctgta
     2341 tctcctacct ctaatacttc tccattttcc attattgcta ctcttccaca aatctgcttt
     2401 ataacttcca tttgatgagt tacaactatt attgttattc ccaatttttt atttatatct
     2461 tcaagcaatg aaagaattga ctttgtagta tttgggtcta atgctgaagt tgcttcatca
     2521 caaagaagta cttgtggatt tagtgctaaa gctctagcaa ttgcaaccct ttgtttttgt
     2581 ccgccactta gatttctggg tttatcattt ttcttttcag atattccaac tacttctaat
     2641 aactctaaaa ctcttttttt aatttctgcc tttgagtatc caaaacattc taatggcaat
     2701 gctacattat caaatacagt ttttctttca agcaatgaaa agtgttgaaa aatcattcct
     2761 aactcttttc ttaaatctct catttgcttt tcattcaatg actttacttc tttatcagaa
     2821 acaagtacac tgccttcttg gtattcttct aatccgttta tacatctcag tagtgttgat
     2881 ttccctgcac cactatgacc tattattcca aaaatttctc cagattctat ttcaatactt
     2941 acatctttaa gcacttgaat ctttccataa tacttattaa cattttttat gctaatcata
     3001 aaatttcctc ccatatattc tgctcagttt ttatgtttat cctctcttat gtattacaac
     3061 aaaataaaag cccgagtata aactcaggct tagaaagcta tttatactca tctatcaact
     3121 atattagttg caggatttag caccaatgta tataaaatat actggttgcc gggtttcaaa
     3181 gggccagtcc ctccaccact cttgataaga cgttatttaa tttaattcta ttatcatttg
     3241 ttgctatgtt atatatgata tattcattaa tagtatttgt caataaattt atttaaattt
     3301 tttatttttt attatcgtta attaactatt tttaattagt tttctagctc ctaacacttt
     3361 atattttaat gtctatataa ctatagaaca tcattataag aaataaatta aataacatca
     3421 atctatctaa atcatctgta aagtaaatta agaaaattaa cattttatcc aaactctatc
     3481 ctactattaa ataaaataat ttaaatacaa tgatgattat atagctctag tactttagac
     3541 tttatcaaat gtttaagttc tataggttct attattgtag catttttccc aaaagttaaa
     3601 aaatattcag ccacccagtt aagtgtagat tttggtatat acatatctat gtatccacct
     3661 ttagacaaga ttttcaatcc tcttgccaac aaaaattctg tttcacaaca ttttactccc
     3721 tcatctgtca aattaatttt aatatgataa tctgtaccta cttccatatt cttaagataa
     3781 tcatgtatag aagataaaac cttatatttc ttacttgata agttttctat actcaaaatc
     3841 ttcacaattc tgtctacacg aaattcctta atagattctg attttataca gtaagctgga
     3901 caataccaaa gtccattcat ggcatacaaa ccaataggaa caatggtacg tacactattt
     3961 ttttgctttg atgaatattc aattttaatt gtagattcat tcattactac attaaacaat
     4021 tctttcaata atggtgttga acagtgccta tctggtgtcc aaaaaacaaa tttacgcctt
     4081 atattctcaa tattatattg aatatcattt ggtatacaat ttaaaaattt ttttaaaaca
     4141 gaaatagttt cttgctcaaa tggcaaatca ttataatatt ctagtgactg atatgcaaag
     4201 aatatagatt ttacctcact ttctgaaaat gcaatcggtg gaagtatacg ctctttaagt
     4261 acatgatatc caccagccgc tccaactttt gaatataatg gaaatccaat actctccagc
     4321 acctgtaagt ctcttagtat agtacgtttt gatacagaaa attcttctgc taattctcct
     4381 gctgtaaaac ttctttttgc atttattgtt atcatcagtt caattaaacg ctcagctctt
     4441 gacatattta tactcctaat ttaaatatag tgacaataat tgtcactatt atatgataag
     4501 atattcttac atttaaagca ataataatag taaagaaaga agggactgat actttatgga
     4561 aaataatact attaaggctg atgatattat caattattgt ctatcaaact tagatgatgt
     4621 tttattaatg aatagttggg gggaacgagc aatttactac aatcctaatg gtattttaaa
     4681 gcgaggagta tatgttctta ccattaaaga aaaagacagt aatcatgata aaggttcgtt
     4741 ggttagtcgt ccaaatgtat accgtgtgaa tataggatta aaaaaagaaa cttttattga
     4801 aatgtttgga tatattccaa agcgtccagg tgtaggtcaa atagttgata tggattttga
     4861 ttttacaaaa ttggacacaa tcatgccaca tcctatctac tcatggatgg gatggatatg
     4921 cgtcctaagc cctactgaaa agacatttga gaactttaaa actttaatag gagaatctta
     4981 caattttgca aaacaaaaat ttaaaaagag aaaaaacaag taaaaacata tgaaaataat
     5041 ccctaaataa attataataa aaatattaag gttacaagat aaaaattaat atcttaataa
     5101 catgcaaaaa agcgaagcca aaaaatgtat tatttataag gcttcgctat atctcaaata
     5161 atttatttca aatttcaaaa ctatttattc tattatttct tattatttcc tgttattcct
     5221 aatctatcat tcatatcttt tatcattttt ctaaaaccac tacctatatc atttccaaca
     5281 tcttcagttc catgaactaa ttttataaag tcattttgac cttttttcat atcttcatca
     5341 gataccttat ctaatttttc tattctctta tctatgtctt ttactaatct ttccccttca
     5401 gcgattaggt catcattcat tttctttaac tctgtatctt ctgttttacc atctttttta
     5461 agagatgtta caaatgattg tattttatct ctactatctt tatatgcagc ctttacatca
     5521 cttagatatt tttcatttcc tggatattct ttatctttaa actgattatt tatactttct
     5581 ggagtatcat aaatagaata tctatttaga cctgctatat aatcattata gcttgttgaa
     5641 taataattca tatatccttc attattattt acagattcat taggattttt tcctgtattt
     5701 tcacttggtt tttcattatt tttagtacat gcagttatac ctatagctaa tactagtgct
     5761 aatcctacaa ataaagttct ttttaatttc attttta
//
LOCUS       DAMJYC010000175         5616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJYC010000175 DAMJYC010000000
VERSION     DAMJYC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.182"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            127..627
                     /locus_tag="PC472_003832"
     CDS             127..627
                     /locus_tag="PC472_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2729105.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            684..1670
                     /locus_tag="PC472_003833"
     CDS             684..1670
                     /locus_tag="PC472_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2729106.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1720..2442
                     /locus_tag="PC472_003834"
     CDS             1720..2442
                     /locus_tag="PC472_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2729107.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2471..4405
                     /locus_tag="PC472_003835"
     CDS             2471..4405
                     /locus_tag="PC472_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2729108.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMLIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4398..>5616
                     /locus_tag="PC472_003836"
     CDS             4398..>5616
                     /locus_tag="PC472_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2729109.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKHLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDI"
BASE COUNT         2343 a          429 c          970 g         1874 t
ORIGIN      
        1 attgggaaaa tagttcacta aaataatttg tttttataaa attactgttt aagtttatag
       61 attttgtata aaataaagtt atgttattaa tttttttaaa aataaaactt ttactaggag
      121 acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat
      181 gcagttttaa cattatcatt gggatttata agttatggac caatacaatt tagggtagca
      241 gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt
      301 tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta
      361 gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa acagaataaa
      421 agtactttag ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta
      481 atgttaaatg tattctttgg gcttccatta attctatcta tgatacaggt aggttttgga
      541 gaatttgtag tagtaacaat tgtaggagta ccatttttct gttttttaat gaaaaaatat
      601 aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat
      661 tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat
      721 atattaatga taagcaaaag tttacaataa aagagttgtc agaggaattt aatctttcag
      781 ccaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag
      841 aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat
      901 ttacagaaaa agaagttatg atcatactat ttgcacttaa gtcacttcag atttataatt
      961 ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa
     1021 gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata
     1081 ctaatgatgg agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat
     1141 ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc
     1201 ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg
     1261 ataaaataat attagttcat atagataata taattgatat aaaaagaaca ggagaaagta
     1321 aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa
     1381 atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag
     1441 aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg
     1501 gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt
     1561 tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt
     1621 atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa
     1681 tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt taataagaat
     1741 gctaaaataa ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa
     1801 ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat
     1861 gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa
     1921 catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct
     1981 ataggaatgg attgttcaga gaaggaaaat aaagaagatt tagagcttgg gagagagttt
     2041 aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct
     2101 atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga
     2161 gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata
     2221 gctgacattt tatttgacag gatggaatat attggtgagg aatttataag taagaaagga
     2281 attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt
     2341 aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa
     2401 aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag
     2461 gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt
     2521 ttatcaggaa acagacacac cacagatttt tagagaagaa agcataattg cacttggatt
     2581 tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat
     2641 taatgagaat tgtaatgata aagattttat aaaaaagttt atgttgatta aaaaaggaga
     2701 ttatttttta tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat
     2761 tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac
     2821 actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag tatatacaag
     2881 tgaattttat aaattaaata ataatgatat tagctcatat ttagaaatta ttaagtcaca
     2941 agataagaaa ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa
     3001 aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc
     3061 cattaatcta gaacaattag aaagtctaaa aaatataaag acagatgatt atattttatt
     3121 gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt
     3181 atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt
     3241 ttttcctgtt agatggatag agtatgaaaa ggagaatata gacggaaatg catgggttat
     3301 atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatgccatt
     3361 aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt
     3421 ttacaatcta gtaagtgaaa ccagtctaag aaacagatac tatacaggtt acttagatta
     3481 taaaaacaat gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga
     3541 gggtcttaag tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat
     3601 atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga
     3661 aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa
     3721 tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga
     3781 tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga
     3841 tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa
     3901 attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat
     3961 agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta
     4021 tgatttattg tctattgtag atggtataga tattagaaaa atgctatata caatcaataa
     4081 aaggatagag tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt
     4141 agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga
     4201 gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga
     4261 aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa
     4321 taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga
     4381 ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa
     4441 gggtaggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaagcatc
     4501 tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta
     4561 gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa atacttccta
     4621 aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag atgctttcta
     4681 tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata
     4741 atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag
     4801 gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat
     4861 tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg
     4921 atgagtatag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt
     4981 tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc
     5041 aaaatgtaga tttaatttat atagatagaa aaaaactgtt ggattataaa ttttataaaa
     5101 acaatgatag atttaaagat tgttatttac tagctagact tctactttta aatttgtcta
     5161 tggataatag tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt
     5221 atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat
     5281 atatacaaga taagagtaaa tttcttttga aaaatgaaca aactggtaag aaaaacttta
     5341 atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca
     5401 ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc
     5461 agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta ttatatcctt
     5521 gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt aagggtaagt
     5581 ttatagaagc aaaaactgtg aggttagatg atataa
//
LOCUS       DAMJYC010000176         5445 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJYC010000176 DAMJYC010000000
VERSION     DAMJYC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.183"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(204..1388)
                     /locus_tag="PC472_003837"
     CDS             complement(204..1388)
                     /locus_tag="PC472_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2729110.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC472_003838"
     CDS             1761..4112
                     /locus_tag="PC472_003838"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2729111.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDNLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC472_003839"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC472_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2729112.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2136 a          613 c          866 g         1830 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttactatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgctg
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgataattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcac aaagaacttt gaaaattaaa cagtaggtta atttataaaa caagaaacaa
     5401 accataaagc cagatatttt gataacaata gtatctgagc ctgat
//
LOCUS       DAMJYC010000177         5345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJYC010000177 DAMJYC010000000
VERSION     DAMJYC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.184"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            138..974
                     /locus_tag="PC472_003840"
     CDS             138..974
                     /locus_tag="PC472_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2729113.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1327..2904
                     /locus_tag="PC472_003841"
     CDS             1327..2904
                     /locus_tag="PC472_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2729114.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEYTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2897..4081
                     /locus_tag="PC472_003842"
     CDS             2897..4081
                     /locus_tag="PC472_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2729115.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGKEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     NL"
     gene            4099..4698
                     /locus_tag="PC472_003843"
     CDS             4099..4698
                     /locus_tag="PC472_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2729116.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4722..5192
                     /locus_tag="PC472_003844"
     CDS             4722..5192
                     /locus_tag="PC472_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2729117.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1992 a          565 c         1028 g         1760 t
ORIGIN      
        1 aaaaatattt tgttattgta gtattatagg gtaaattgca aaatatatac gaaagtaaaa
       61 ttttaaatat tgacattaaa ttaaatatac tatactatgt aagtgtgatg agagaatttt
      121 tttagagggg atttatagtg aatataaaaa agatatttaa taacaatgta gtagtaagct
      181 ccctagaaga tggcacagaa ataattgtca ctggtgctgg agtaggattt aagaaaaaag
      241 taggcgattt gatagatgaa aatttgattt ctaagaaata ttttgttcaa gacgatcaaa
      301 gggacaaata caatcaaata cttaataaga cctctattga atactttaaa atttcagaag
      361 aaattataga aaaggctaat gaagttttaa atactcaagt gaatgattca ataatattag
      421 ccttaactag tcacatagaa tttgctgtcc aaagagaaaa acaaggtata aaattaccaa
      481 acttaatatt gaatgagaca aaacaacttt atagggaaga atttgaattt ggattatggg
      541 caattgatga aattgaaaaa aggataggaa taaaattgcc tgaagatgaa gcaggatata
      601 ttgctattca cataatcaat ggtttggaaa attctcaaaa agatgaaggt attaatatac
      661 tagagttttc aaaacaagtt attcagataa ttgaagaagt acatggattt aaattggatg
      721 taaattcatt aaattatact agacttataa ctcacttaaa gttttttgtt caaaggattc
      781 tccgaaaaga aacttacaat gatagtgata ttgaagatat gtataagtta gtgaataaaa
      841 attacaataa acctaaagtg tgtactgaag aaattgcagc attggtatta gacagatttg
      901 aatataagat tagtaaagaa gaagaattat atttaatgat tcacataaat aaaataacaa
      961 acagtggttg ttaggattgt aactgataaa gcaggcaaaa cctaagtcat tgagcttaac
     1021 tattagatta agttttttga tttaggtttt tttattagat acaatcaaaa tacagacagt
     1081 gacttttagg attgtaactg gttaagcagg caaaacctaa atcattgaat ttaactatta
     1141 ggttaagttt tttgatttag gtttttttat tagatacaat caaatacaga cagtgacttt
     1201 taggattgta actggttgag caggcaaaac ctaaatcatt aaacttaatt attaaattaa
     1261 gttttttgat ttaggttttt ctataaattt gattatgttt ttagataaaa ataggagggg
     1321 aaaattatga aaaaaaataa gttaggttta tggaacttct tccaaatgtt aggaaaaacg
     1381 tttatgttcc caatagcact acttagtgtt tctgggatga tgctaggact tggggcaggt
     1441 tttacagacc ctaagatgat tgaaatggtt ccatttttag ggaatgaata tgttaactta
     1501 gttttaaact tcatgttaac aataggatta tttgcattta acaacttggg agcattattt
     1561 gcaatggcaa ttccactagg attattaaaa aaagaaaaag agtttggtgc tttttcagga
     1621 ttagtaggat ttatagcaat gcacataggt acaaattttt atttaacaag agctgatttg
     1681 ttagtaccag cagaacagat gtcaactaat ggtcaagcta tgattatggg tatacaaaca
     1741 tataatacaa gtgttttagg tggtattgta gcaggattaa ttgtttatgc aatatatgat
     1801 aaagttagta atttaaagat accagaatca ttaggattct acagtggacc aagattagtt
     1861 ccaattgtta gtttaatagt catgagtata gttggattat taatacctat tgtttggcca
     1921 ccatttttca atgcattcca aagtttagga agatggattt cttcagcagg accattagga
     1981 tatttttcat atgcagtggc agaaagagtt acaattccat ttggactaaa tcatcttgta
     2041 acatccgtat tccgttttac accaataggt ggaactgctg ttattgatgg agaaacgtac
     2101 tttggtacat tgaacatgtt tatggcatat gtagaaaata atcagattat acctcttgat
     2161 ttagctggta aaatggaaca aggtaaattg atgattcaat atggattagc tggtgctgca
     2221 ttggctatct accgtacagc aaaaccagaa aatagaaagg cacttaaagg acttttaata
     2281 tcaggagtgt tgacagttat tataggtggt ataagtgaac caatagagtt tctattctta
     2341 ttcatatcac cagcattatt tgctttccat acatttatga atggtctagc aaacatggta
     2401 cttccatact taggcgtgtt gatgggattc acaggagatt taattgcatt tataagtttt
     2461 ggtgtgttaa gaggaacagc tacaggatgg ccaatagcag tattagtagc agttttatat
     2521 tttgcaatat actattttgt atttaagtgg gcaattatta agtttaatat aaagacccca
     2581 ggacgtgagg ataaagaaat aaaagtatct gaaggaaatg gttcagattt caaaaatttg
     2641 tctacatata aaggacaaca aatgatatct gctttaggtg gtcaatctaa tatattatca
     2701 ctagataatt gtgtaactag attgcgttta aaactacaag atgtatcatt aataaatgag
     2761 gatgcaatta aagaggctgg tggtatagca gttgttaaat tagatgagta tacaatacaa
     2821 gtaattatag gaactcaagt gtattcttta agaaagcaga tggataaagc tatggaaggt
     2881 catttggagg catgtaatga ttaattttga taaaaaagta gaccgattgg gtacatattg
     2941 tacccagtgg gactatgtag aagatagatt tggtaagaag gatttgcttc cttttacaat
     3001 ttcagatatg gatttagagt ttccatttga aataacagag gtgttgcagg aaaggttaaa
     3061 tcatagagtt ttgggataca gtagatggaa acatgatgat tttagaaatt cagttacgaa
     3121 ttggtatatt aaaagattta attgtagcgt tgatagtaac tggatatatt atagtccaag
     3181 tgtaatgtat tcaattagca aacttattga gatgttaagt aatgaaggag atggaatcct
     3241 tataaatact cctgcataca atgcttttta tgatgtgata acaaataaca atcgtaatat
     3301 tataaagtca catcttgtaa ataggaatgg attctattca attgatttta atgattttga
     3361 gaaaaagtgt aaaaaatcta agatatttat tttatgtaat cctcacaatc caactggtag
     3421 agtatggagt gagttagaac ttgttaagat gataggaatt tgtaagaata atgatgtcaa
     3481 gataatatca gatgatatac acatggatat tgtatatgac aatagaatga cacctatctt
     3541 aaatgttgca aaggactatt tagattctgt attcatttgt acatcagcgt ctaaatcttt
     3601 caatatacca gcattgactg ggtcatatgt gattatacca aatgaaggta ttagaaataa
     3661 atttgaaaat atcactagat accgtgattt tgtaaattcg ccagcaattc tagcaatatt
     3721 agctacaatg gtctgttatg atgactgcga atattggtta gatgaactat taaaatacct
     3781 taaagagaat cttaaatata cgattgatta tataaaagat aatttagaac cattgaaatt
     3841 ggtcatgcca gaaggctgtt actttgcttg gatagatttt tccgaactta aaataagtgg
     3901 tgagaaattc caaaaattat tgatggatat tggagaagtt gctattatgt cggggtctgt
     3961 atatggtaaa gaaggtgagt tttatttaag gttaaatgtg gcatgttcaa gagaaaaact
     4021 tgaagatggt ttaaatagga ttaaaaaaac tatggattat attgcaaaaa gaaatttata
     4081 gtaggaaggg agatagttat gggatattta aaaacaatgg ttgatgttat gactactgaa
     4141 atgaatgcca taagatgttt aatagatgag gcaggaatag aatatgaaag tatagttagt
     4201 gaaattgcaa attgtgaagg caaagtaatt tttatgggtg taggaaaatc tgcacatata
     4261 ggtaagaaat tggcagctac atttgctagt actggtacac ctagcttttt tgtacatgca
     4321 acagaggcag tgcatggaga tttaggaatg attgaaagta aggatattac aattttaata
     4381 tcaaatagtg gaaattctat ggaagtggta aactgtataa aatatattaa agcaataggt
     4441 tctaagacaa ttgcatttac ttcaaacaga aattcagtac tggcaaaaga gtgtgattat
     4501 gctttaatat atcctgcaaa ggatgaagca gaccatttaa atctagcacc aacaacatca
     4561 tctacaatta ctttggtttt aggagattct attgcatgtg cattatctaa gagtagtaat
     4621 tttggttctt cagattttta taagtaccat ccaggaggaa gtttaggtga gaagttaaaa
     4681 acagctaaca atggataaaa gcaaaaagga gagtagtttt tatgttcaaa atgtttaaaa
     4741 aaaaatcaat aaaatcaccc atatcaggaa aagtaataga gttatctaaa atacaagatg
     4801 gtgtattttc tcaaaaaatt atgggagagg gtgtagctat agattctaca ggagacattg
     4861 tgtatgctcc aatcaatgga aagatagtag tagtagcaga gactaaacat gcatttgtaa
     4921 tagaattaga aaatgggatg gaacttctta ttcatgttgg tttagatact gtaaatttaa
     4981 aaggcgaagg ttttgaggca ttggtgagta tgggagaaca agtgaaggaa ggaacaccaa
     5041 tgttgaaaat taaccgtcct ttgattgaat caaagggtat atcacttatt actccagtta
     5101 caataactaa tcattcagag tacaatatga atacatgtaa tgtaggcaat agtgttgaag
     5161 gtgggaaaga tactgtaatt gagtttaaat aaagaactaa aatacaaggt taatatttat
     5221 aaatacctat aaacttatgc tttataagat tgattgtata gatttgttgt atagatttgt
     5281 tgtataaaaa agaactcttt aattataaat taaggagttc ttttttattt tgtaaatatt
     5341 gttta
//
LOCUS       DAMJYC010000178         5002 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJYC010000178 DAMJYC010000000
VERSION     DAMJYC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.185"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            701..4843
                     /locus_tag="PC472_003845"
     CDS             701..4843
                     /locus_tag="PC472_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2729118.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDAVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVVSADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDLPASIINPEYSLDGGVTFQPWNGSLNIGTLEVGETK
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVIFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKNCCCNCCKNDF
                     WC"
BASE COUNT         1937 a          727 c          834 g         1504 t
ORIGIN      
        1 tattatattg attattcaaa attatatata aaattcagaa aaaaatttta gtttacataa
       61 taaaaataaa gtcaactaaa attattgatt ttagattaaa gtaccggatt aagcatttaa
      121 gtagatttta agcttgattt gattagaaag gtacaaacta tcaccgaaaa aaattaaacc
      181 catttaaaaa gcaatataaa agcttgttat ttttgagaaa aatagaccct ctaaactcaa
      241 ttagattaaa aataaaatac agaaaattat atctctaaat atcttaaata attaagttta
      301 aattttacga tattttgttt gatgatttat aatataaaag catactctta atcttcaaaa
      361 tttaaaaaga gtaaaaaata acaaaaaaat cactgacatt tcactgacaa attgtaattt
      421 tgttattata aatacattga aaatacttga ttattgaaaa atggcataaa ataatacatt
      481 ttatgccatt tactattatt tgatagtttt taacttatga cataagatat tttttaacat
      541 atacaataaa atataaaaaa attgatattg caaataaaaa aaatacatat tagatgttat
      601 agaaaaaaag cgcacttata ggcttaaaat gtcacataaa agagttttaa agaataatat
      661 attatgaaca caaattttgt aatcacaaag gagtgataat atggcaacag tatcaggaca
      721 aatagttttt gatagaaata gaagtgctac cattgattct ggagactcag gaatagccaa
      781 tataccagtt gtattacaag atatagcaac tggagttaga cttgtggttc tcacagatgc
      841 atcaggaaat tatgttttta ctaatgttcc aaatggtaac tatagaattg tagaagcttt
      901 tggaactaca ggaggagtac caacaccagg gaattttgca acagcgatag taggtccagt
      961 tccaattgca gaaatgccac ctattagttc tgtgacaaat cctcctattg gttcaacaaa
     1021 cttagatggt acaacaccta acacactatt aataacagta gcaggttcag atataacaaa
     1081 tcaaaatata ttaaatgggc ctgtgattta tacacctata gagaatatat tggattcttg
     1141 cgtatctgta tctaatataa accttattac agatgcagat aacggaacat tcggtttttt
     1201 tccaccaggt acaccaagta atacaggacc tgaaatagaa ccttacccaa atgtgacgcc
     1261 agattttacg tatgttgtac cagacccaac taagtttaca cctttagatg gagaatatac
     1321 agtacaaaat attatgacaa atgcaatgag taatgttata ggagcttggt ggcgtattgc
     1381 tgaccatact acaggaaatg aaacaggaag aatgatggtg gtaaatggtt ttaatcctgg
     1441 agctgtattt tttaaatcaa cagtacaagt tacacctaac actaattatt tatttagcac
     1501 ttggatttta aaccttttta aggtaacagg atatccacca ccacagctag gtgtaagaat
     1561 atttgaccaa aataataata tactatatca agcaacatta ggagctcaaa ttccagttaa
     1621 tacaaacgca ccagaatgga aagaagtagg tactgtgatt aattctcaaa ataataatca
     1681 acttacagta gaatttttta gtgaaggaga aagcgccatt ggaaatgact atgctataga
     1741 tgatgtttct ttaagagaaa tagctgttcc aacttttact ccaataaaaa caagtaacac
     1801 agaaactgct aatgtaggtg atataatcac atatacagtt acattagata atacttgtga
     1861 tagcccttta actaatgtat tttttaggga tttaattcca aatggtttag agtttatacc
     1921 tggaactgta acggttgatg gagtatctgt gccaggagta gaccctaata caggtttccc
     1981 acttccagat attggaggag gaacaggggt tgaagtaact tttgatgctg tggttgaaag
     2041 tattcctaac ccaaatccta ctaataatat agctaatata gattactcct atacacctgt
     2101 agaaggcggt ataccaaatg atttttcagt agattctaat ccagttcctg ttgaagtagt
     2161 atcagcagat attgaagtta caaaattatc tgaacctact atagtgaatc caggagagga
     2221 attaatatac actataaaag tagtaaataa tggaccattc ccatctgaga atgtggtttt
     2281 aacagatgat ttaccagctt ctattataaa tccagagtat tcattagatg gaggagtaac
     2341 ttttcaacca tggaatggaa gtttaaatat agggacatta gaagtaggag agacaaaagt
     2401 tattataatt aggggtattg taaatccatc aacagtagga attataacaa atacagctgt
     2461 agtatcttca actacagcag acccaaacct taataataat acatctacaa tagagacaga
     2521 agtgaattta ttagcagata ttttagtaat gaaaacagca gaacctaatt cagctgttcc
     2581 aggtacttta ttgagatata ctattcaagt agaaaactta ggaccagcaa atgctgaaaa
     2641 tgtaatatta aatgatgata ttccagcttc tattataaat ccagaatatt cattggatgg
     2701 aggagcaagt tttcaaccat ggaatggaag tcttaatata ggaactttaa actctggaat
     2761 ttcattaaca gttttaattc aaggaacagt tagtttaaat tcttcagaat atatagtaaa
     2821 tacagcaaca gtttcttcaa ctacacctga cccagattta agcaacaata tatctaccat
     2881 tattacacca gttaacccac aagcaggtat ttctattata aaagtagctg acgaggatgt
     2941 agctgttcca ggagaagaat ttgtatatac aattgaaatt tttaatgaag gaccatctaa
     3001 tgctacgaat gtagttttaa cagatgatat accagatgtt attttaaatc cagaatattc
     3061 attggatgga ggagcaactt tccaaccatg gactggaagt cttaatatag gtacagttgc
     3121 accagggcaa ttaataagaa ttatcataaa gggattagta agttcaactg ctacaggaga
     3181 tattactaat acagcagaag taagtgcaga tgtaccagaa ccagttacgg attcatcaac
     3241 agtgacaact cctatagttc catcagcaga tatagaagta ataaaaacta gcaatatgga
     3301 tacagcagtt ccaggagaaa ctttcagtta tactataaca gttacaaatt taggaccatc
     3361 tgctgcacaa agtatcgttt tgacagatga tataccagat gttattttaa atccagagta
     3421 ttcattggat ggaggagtaa ctttccaacc atggaatgga aatcttagta taggcacttt
     3481 ggatgctgga gaaataagaa gtattataat aagaggaaca gtgagccaga cagcagtagg
     3541 tacaattata aatacagcaa caataagttc aactacacct gacccaaatc cagataataa
     3601 tacttcaaca gatgaaacag atataagtcc attagcagat atttctgtga taaaaggcaa
     3661 cgaaccagtt gctataccag ggggaagatt tatttatgga attgaaattg caaatgcagg
     3721 accatctttt gcagagaacg taactttaac agataatata ccagcttcta ttttaaatcc
     3781 agagtactca atagataatg gagtgacttt ccaaccatgg aatggaagcc ttaatatagg
     3841 tactttagat gctggagaga taagaaatat cataataagg ggaacagtaa gtcaaacagc
     3901 aataggcaca attataaata cagcaacagt aagttcaact acacctgacc caaatctaaa
     3961 taacaatatt tcaacatcag aagcagaggt aagttcatca gcagatattt ctgtagtaaa
     4021 acgaagcaat cagactgttg ttgtaccagg agatgtacta gactatacaa ttgaggtgag
     4081 aaatgcagga ccttctactg ctcagaacgt aactttaaca gataatatac cagcttctat
     4141 tttaagtcca gaatattcaa tagataatgg agtgattttt caaccatgga atggaagtct
     4201 tagtataggc actttagatg ctggagagat aagaaatatc ataataaggg gaatagtaag
     4261 tcaaacagca ataggtacaa ttataaatac agcaacagta agttcaacta cacctgaccc
     4321 aaatccagat aataatactt caacatcaga aatagatata agttcatcag cagatatttc
     4381 tgtaataaaa ttagcaaata aaacagaagc atgtgtagga gagcaaatag attttgtaat
     4441 agtagtatca aatgcaggac cagaaaatgc tcaaaatgtt actttaatag acaatgtttc
     4501 agataagctt aagaaagcag tattttcact tgatagaggt gtaagtttcc aaccatggac
     4561 tggaagtctt aatataggta cactaccagc tggaacttta agagttattc tattaagagg
     4621 aattataaaa tcaacatgtc ttgatagatt aacaaatatt gcagaagtta cttcaacaac
     4681 accagattca aaccttaaca ataatatatc aagagtgcaa gtagaaataa aacaatgttg
     4741 ttgtaatgat tgctgttgta acaactgtag taattgttgt tgcaatgatt gtggttgtaa
     4801 aaattgttgc tgtaattgtt gtaaaaatga cttttggtgt tgattttata aaataatttt
     4861 gtatacctta aattaaactg aaataaaaaa taaattcaca tatgagtaaa atattttata
     4921 aatattaata aattttctat ctttgatatg gaccacattc gtaatactag ttattaaatg
     4981 aagtggtcct ttttaattca tt
//
LOCUS       DAMJYC010000179         4998 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJYC010000179 DAMJYC010000000
VERSION     DAMJYC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.186"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            24..1907
                     /locus_tag="PC472_003846"
     CDS             24..1907
                     /locus_tag="PC472_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2729119.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2131..2811
                     /gene="pgmB"
                     /locus_tag="PC472_003847"
     CDS             2131..2811
                     /gene="pgmB"
                     /locus_tag="PC472_003847"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2729120.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2808..>4998
                     /locus_tag="PC472_003848"
     CDS             2808..>4998
                     /locus_tag="PC472_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2729121.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSD"
BASE COUNT         2057 a          454 c          952 g         1535 t
ORIGIN      
        1 ttataaagga gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta
       61 atcaaatcaa aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa
      121 tgagcaatta tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa
      181 aaaatgaagc gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag
      241 gtgcaggagg aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag
      301 taatgagtaa agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt
      361 taaataaacg ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta
      421 cagaaaaaag aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag
      481 gtaatttttt tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg
      541 tgaaagatac tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta
      601 taaacccaga aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg
      661 gagatttaca aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa
      721 tttacttttg ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata
      781 tggaggtaga ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc
      841 ataagagaaa tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta
      901 aacagtggca agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata
      961 taaaagatat gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt
     1021 atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa
     1081 taaaatattt attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga
     1141 gattagatgt ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca
     1201 aaaaagtaaa accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggt
     1261 taatgggtga ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat
     1321 tcttttgtac agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg
     1381 taagttatcc aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta
     1441 caagaatatt gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta
     1501 tgtttgtaca aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag
     1561 aattaagttc aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga
     1621 ataaggatat atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac
     1681 ttaggtctat atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga
     1741 aaaaagaatc tatttctata ataataaata attcatctaa aaatataatt ttgacgttac
     1801 cagattattt agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa
     1861 aagaagaact aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat
     1921 ttattttaga tttcatgtta aattattata cagtttaaac aagtctttat aaacgataaa
     1981 aaatatggtc tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag
     2041 tagataattt tataaataat atcttaaata caagatagaa aataaattga ttattagtct
     2101 aaaaatttct tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat
     2161 ggtgtaatta cagatactgc atattatcac tatatggctt ggagaaaact agctcataaa
     2221 gttgggatag atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag
     2281 tcattggaca ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa
     2341 gtgagaatgg ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca
     2401 aatgatattt tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag
     2461 ataggtctat cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt
     2521 gacaaatttg attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag
     2581 atatttctta tgtcagcgaa agggcttaat gtaaatccac aaaattgcat agggatagag
     2641 gatgcaagtg caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga
     2701 aattatgaaa acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt
     2761 gaatatatac aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat
     2821 ttagtaatgt agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat
     2881 tagcagagtc cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg
     2941 aaaattatag tggagataca cacaaggggt cttatatagc tggagtatgg tttccagata
     3001 aaactcgtgt tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt
     3061 cagtcaacta tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat
     3121 gtaaagtaga agattttcat agagaattag atatgaaaaa tggtatttta aatcgtaggt
     3181 ttattgtaaa tataaatgat aaaaaatttg aagttagggt tactaggttt ttaagtatat
     3241 ctgttaagga acttgcagta attaaataca gtataaaatc attaaattct aatactaaaa
     3301 taaaatttgt accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa
     3361 agttttggaa tggaatttct tcaagttctt tagaatataa agggagtatt ctttctcgaa
     3421 caaaggagaa tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga
     3481 caaatggtga tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga
     3541 taacaatgga tgttttaaaa aatgaggttg caagtataga aaaatatgta tctataacct
     3601 caactagaga ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatagag
     3661 aactatctaa aagttatgat gatatattaa atgaacatat aaagttatgg aataaaagat
     3721 gggaaagtgc agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata
     3781 atttattcca actattttca acatattatg gagaagattc gcgattaaat ataggtccaa
     3841 aaggatttac tggtgaaaag tatggaggag ctacttattg ggatacagaa gcattttgtg
     3901 ttccagtata tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt
     3961 acaatcagtt agaacaggca aaggataatg caaaaaaact aggtcttaaa ggtgctttat
     4021 atccaatggt aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg
     4081 aaatccaccg aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg
     4141 actataaata tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg
     4201 caagtagagt tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc
     4261 caaacgagta tgaaaacaat atcaataata attggtatac taattatata gctaaatggt
     4321 gtttagatta tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg
     4381 acagaaataa tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt
     4441 atctgctgta tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag
     4501 aatttgtaga agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt
     4561 gggataagat tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact
     4621 attttggaaa tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat
     4681 atactgtaca tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag
     4741 ataacctaga aaaggcttat gaattatatt caagaacagc taggcttgat ttagataatt
     4801 acaataatga tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca
     4861 ttgtacatgg atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt
     4921 tgccaaaaga gtggaaaaat tattctttca atataaatta tagagataat attattaatg
     4981 tagatgtatg tagtgatt
//
LOCUS       DAMJYC010000180         4852 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJYC010000180 DAMJYC010000000
VERSION     DAMJYC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.187"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(181..357)
                     /locus_tag="PC472_003849"
     CDS             complement(181..357)
                     /locus_tag="PC472_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729122.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(411..500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(680..880)
                     /locus_tag="PC472_003850"
     CDS             complement(680..880)
                     /locus_tag="PC472_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729123.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(882..1232)
                     /locus_tag="PC472_003851"
     CDS             complement(882..1232)
                     /locus_tag="PC472_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2729124.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1394..1583
                     /locus_tag="PC472_003852"
                     /pseudo
     CDS             1394..1583
                     /locus_tag="PC472_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2060..2230)
                     /locus_tag="PC472_003853"
     CDS             complement(2060..2230)
                     /locus_tag="PC472_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729125.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(2619..3113)
                     /locus_tag="PC472_003854"
     CDS             complement(2619..3113)
                     /locus_tag="PC472_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2729126.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            3918..4295
                     /locus_tag="PC472_003855"
     CDS             3918..4295
                     /locus_tag="PC472_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2729127.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            4400..4775
                     /locus_tag="PC472_003856"
                     /pseudo
     CDS             4400..4775
                     /locus_tag="PC472_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         1982 a          570 c          565 g         1735 t
ORIGIN      
        1 ttgtgtatta ccacaaatac tattgtatca tagattaaca taaatgtcta tttaattata
       61 atttttagtt aacattttta tttattctta atatgaaaat aaatttaaac attttatatt
      121 accaatatat taatgtaaat gtagataaaa ctaaaaaggg ttttctagaa aaaaatctgt
      181 ctaattatac ttacttacca ataccatcac ttgatgcaag caagattcca caactttttc
      241 tccgtactct aatataaaat catctaactg tgtatctata aaatcacaat aatcatatga
      301 actaccaata taagcagtta acatatgtgt acatttatcc attttatctc taaacataat
      361 gtatttatcc ccctaaaaaa ctttcatgaa aaacaaaaag aaaatatctt tttggcaact
      421 ggctgacata acttatttta taagcgttag tccctatagc tttgcgtcac taaatttctc
      481 tagttttgcc gatttagttt tattctacag attaataata caatagaatt agttattatt
      541 caacatatct agctgaaaaa ttaataaatt tatatatgaa cgttaaatta tctgaaaaaa
      601 taaaaagccc ctctgtcagg agtgacttaa aaattgaatt taagtttgta tttaaaacac
      661 atttttaata atgcaaaaac taatcaaaaa taaacaattc atcaataggt gtatctacta
      721 tttttgatat atctattgct aatttcaaag aaggattata ctttcctgct tctaaacgca
      781 taatggtttc tcttctaact cctacttttt ctgctaattg ttcttgagtt aattctttta
      841 attgtctata tttttttagg ttacaagtga atttaggcat attatttttc ctctaaaata
      901 taaagtttta tagcataaac atttaaagtg attgcatatg taattgatag taaaacacac
      961 attaattcta tattcctaga cactatagta atacaccata aaatgaataa ttctataata
     1021 gcaagctgtc ctgtaaatgc catagctgct ttttcatctt gtacatacct ttcatctgct
     1081 ttatcaccat ttatttttgc tataataaag tttgagaaaa aagcaaaaaa tccaatatat
     1141 gtaaggtcag caacattgcc agatgaaaaa tatctaagac ttaaaaatcc aaggaaacca
     1201 caaaaaccca taaaaaattt tttctgaatc attttttatc ctccttagta attttaaaag
     1261 agatatattt atatctttat gatacaaata tatctctttt atattctttt gtcaatagaa
     1321 aatcagtatt atacaatatt tgtactgcac cataattgtt agagcaaaaa tctactgctt
     1381 gagatgttta tgtatgacta aatatgattt taatttcaaa ttaaaagtag ctaaatttta
     1441 tttgaataga gaaggaagag atattttaat agtaaaacaa tatggagttt ataaccattc
     1501 tcaagttgaa agatgggtta atatattagg agagtgtgca ctaaaaataa aaaaaatttt
     1561 ctatactata tggttcaagt tagatgttat aaactatatg atgataacta aatatagtgt
     1621 acaataaatc gctaagtatt ttggaataaa caatagcaca gttatagcaa catgaaaata
     1681 aaaattttta catagtggtg ttgatgcctt ttctaaaatg aaaggaagac cataatgaat
     1741 aaaaacaaaa acactaaaga aaaaataaat taacaagaga acaagaatta gaatatgaaa
     1801 ataaattttt aaaagatgag cttgagtttt taaaaaagat gtgtgcctca gggaagccac
     1861 attaaaaaac agtggtatca ttattaatca aaagaaagtt agatgtatta tcaaaaaatt
     1921 agaacttaaa tgcatagcat tttttcataa atcacataaa tataactcct ataaatgtat
     1981 aattggaaaa gtaaaaaata ataaaacaaa tagaagattt aaaatatcaa tagcatatca
     2041 gaaaataaca aatgatatgc tagaatttat aaaatatatc taatgataaa aatggaaaat
     2101 caattataaa aaaggcatat cccgattcat tttttgttat atagtcaaat aagaatacta
     2161 tagatgaaag atacgatttt gcatatgtat taagtaagta ttctgatgtt tttggttgat
     2221 acacatgcaa taaagaagtt gtgtttgcag ataaattagc aaaactatag tataagttgc
     2281 aacaataaag aaaatgaaaa ttatcatact aaagcataag gcatgttaca attttattag
     2341 ataggacatt aacatacatt aaagtacttt ttattataaa gagtacttat ttttttgaaa
     2401 ttcattaatg tataaactat caagaatatt actcaacata atttaaaaac aacttgaata
     2461 ggtattttta aattatgtga tataataaaa aatagaagtg tatgccaata cacttctgta
     2521 attataacta agcattctat tcttgaggag tgcttttttc atttctccaa acaagattgt
     2581 ttatttcatc agcaattatt aaataaaatt tattgtattt aagtattcga tttatcattt
     2641 aaaaatttta ttacaatatc tattttgttt tgctcgttca ttatgtctat tgaacttcca
     2701 taatcacttt ttaatttatc tagaccactt ttgcttattt ctccttctgg aagtatactt
     2761 aattctagat atttttcatg accaggagat gacattaata gatatgaacc attataaggc
     2821 atacctaaat tttctatttt atctgctaat gggtcatctt taaagccacc atttattatt
     2881 atttttccat ctttctttac ccatgatatc attcctccat cagtatattt tgattcatct
     2941 gcatagtagc taagagacgc actttttatt gcacttatat cagcttcaag tttagctatc
     3001 tttgcttttt ctatattttt aaataatgct ggaagtgcta ctattgccaa tattcctatt
     3061 atagcaatta ctactaataa ttccactaga gtaaatcctt ttttattttt catttatttc
     3121 ccctccatat aaaattaata atatataaat ttttattttg ttgatttcta ctttctatat
     3181 tttaccatat atagacaaaa aaatcacttt ctatgacaaa tatactatta ttttacaatt
     3241 tatggaaatg atttaaaatg acatttcttg ttagaaaaga tataagaaat ggattaaaat
     3301 aaagaaaact acagtcatat caatgtcaat aatattattt aaaagcagat gaaaataaaa
     3361 agaggactca gaaaataatg tctatttaat tatacttact taccaatacc ataacttgat
     3421 ataaacaaga ttatactaca ttttttttat gctctagtat aaaatcatct aactgtgtat
     3481 ctataaaatc aaaataatta tatgaactat cagtataata agtcaaaata tgtatacatg
     3541 aatacatatt ttaaaagata tatattatta catacctaaa aaactttcat aaaaacaaaa
     3601 ataaaatatg cttttagtaa cttgttaaca taacttataa tttattttat aggcattagc
     3661 ctatatagct ttgtgctact aaatttcttt gattctagcg atttagtgct attctacaac
     3721 taaaataata caatagagct atttattgtt tggtagcatt gtttgcaaat taaacaaatt
     3781 tatatatatg aatattattt tacgtttaaa aaaacagact aaatatgtag aatatttggt
     3841 aaaacttacc tctagttttt gtcttaaaaa gctcctatta ttaatttaag ttagaagaat
     3901 taaggaagtg acataaattg aaagaagaaa tattaataga aaaattatta agagtagaat
     3961 taatagtaat ggagtattta tggaaaaaag acactctaat gcctaaaaaa aaaattgtaa
     4021 aagaaataaa acagatttat ggatggcata aaagtacaat aaaaatacta ctaaaaaggc
     4081 tagttgataa aaggtatttg gctagatata ttataaattt tcaatcttat tataaaataa
     4141 taatagataa taaagaatat tatactttta agaaaaaggt actaaaatct agtaaaagca
     4201 taaaaataat gcattcactt accactatac ataaaaatgt aagtaaagag aagttagacc
     4261 tattagatga gtattataga aacttaaaag aatgatatag taaaaatcga atgtaattat
     4321 ttaccttaat tcctttgttt gtaaaacttt attgatatag aatttgacat atacataaat
     4381 tggtaaaggg atgtatacta tgaaaattag taaattacca gaagcggaat taaaagttat
     4441 gagatatatt tgggaatcag aaaaggtatt aatatcaaga gaaatcacca agtctatgga
     4501 acaaaaatat gaatggaaag atacaactat atttacagta ttaaaaagat taataaagaa
     4561 gagagttttt agaaacggag aaaatagata agtgtacaca ctataatgta ttagtaaaag
     4621 aaaaataata tcagagattt taaacaaaag aatttttaaa gaatacacat aaaaattcaa
     4681 tattaagttt aatgtcagcg ctatgtagta aggatgatga agtagatggt gatattttaa
     4741 atttattaga agaatatttt aagaatttga aataagaaaa tgacaaattg atacataata
     4801 aataaaaaga aggtatgaaa ttataacttt ttctaccttc attttgttta tt
//
LOCUS       DAMJYC010000181         4729 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJYC010000181 DAMJYC010000000
VERSION     DAMJYC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.188"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            180..1331
                     /locus_tag="PC472_003857"
     CDS             180..1331
                     /locus_tag="PC472_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2729128.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1377..2552
                     /gene="thiI"
                     /locus_tag="PC472_003858"
     CDS             1377..2552
                     /gene="thiI"
                     /locus_tag="PC472_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2729129.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEETTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2688..3491
                     /locus_tag="PC472_003859"
     CDS             2688..3491
                     /locus_tag="PC472_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2729130.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3734..4375
                     /locus_tag="PC472_003860"
     CDS             3734..4375
                     /locus_tag="PC472_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2729131.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYSAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1905 a          451 c          912 g         1461 t
ORIGIN      
        1 tataagtata ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac tagcttttca
       61 tgaaataata ttttataatt taagattgtc tttttaataa agattaacaa aaaaaatact
      121 ataattagtg gtataatagt tagagttgaa gattttaaat aaaggtaggt gtaattaagt
      181 tggaaatata cttagacaat agcgcaacta caaaacctta tcaagaagtt atagataaaa
      241 tggtttatgc acttaatacg gaatatggaa atccttcttc tgtccataga aaaggtgtag
      301 aagttgaaaa agctataaaa gaagtaagac aagatatagc taaatctcta ggagcgaaag
      361 aaaaggagat ttattttaca tcaggtggta cagagtgtaa taatactata ataagaggta
      421 ttactagttt aaataaaaaa agaaaaaatc acataatatc tactaacata gaacatcctt
      481 ctgttttaaa tacattaaaa gatttagaag aagatggttt tgaagtaact tatttagagg
      541 tcggaaaaga tgggaaaata aatatagagg atttaaaaaa tgcgataaag tcaactacat
      601 gcttagtaag catgatgcat gttaacaatg aaataggaac tatacagcca ataggtgaag
      661 ttggaaagta tttgaagggt ttaaaagaaa aaatttattt ccatgttgat gctattcaat
      721 catatggaaa aattaacttt agaccatcta aatacaatat agattttatg agtgtaagtg
      781 cacataaatt tcatggtcca aagggaattg gatttatgta tataaaagaa aataatcgcc
      841 ttaagcctat gttaacaggt ggtggtcagg aaattggaat taggtcggga acagaaaatg
      901 tacctggaat atatggtctt ggagaagctg ttagaatact aaacaaagat ttagatgctg
      961 ttatttcgaa agtagataac ttaaaaaata tattaaaaaa tgaaataatt gataatatag
     1021 aagatataaa aatcaattct ccagaagatg gagtatgtca tatattaaat gtatcattta
     1081 gagggacaaa aggagaagtt ttacttcatt acttagagca aaaaggtatt tatgtatcaa
     1141 ctggctctgc atgttcttct aaaaagaaag gtagttacgt attaaatgct ataggtctta
     1201 caaatgaaga aataaatgga acaataagat ttagtttatc tgatatgaat acagaagaag
     1261 aaatgttaga agctgtgaaa gttttgaaag aatcaatttg tgacttaagg tcaataatga
     1321 aaagaaaata aagaatactg tataaatagt gataataaat tagtttaaga ggtgaaatgg
     1381 tgtataatat attaatagta aagtatggag aaattggagt aaaaggaaag aatagatata
     1441 tatttgagaa taggcttata agaaatataa gaaatatgtt aaaaccaatt ggaagattta
     1501 atgtatataa agagtatgga agaatatatg ttgatttaga agactatgat tatgaagaag
     1561 taattgaaga agttagaaaa gtatttggga tagtgggtgt gtgccctgga gtaagagcta
     1621 aaaaagatta tgatacatta aaagaaatag ctttaaaaat gttagaagaa aaaatagagg
     1681 ctggatataa aacttttaag gtagagtcaa gaagaggaga taagtctttc ggattgactt
     1741 ctcaagaaat gagtatggat ataggtggat acttattatc aaaggtagga gataggatta
     1801 atgttgatgt aagaaatcca gaagttaaaa taaagtgcga atacagagaa tttcatacta
     1861 tggtctatag tgatactata cctggatatg gtggattgcc acttggaacg aatggtagag
     1921 ctatgtctct tttatcagga ggtatagata gcccagtagc tacatggatg gttgcaaaaa
     1981 gaggtatgga agtagaggca gttcactttc atagttatcc atttacaagt gaaaggtcac
     2041 aggaaaaagt taaggattta gctaaaatac tggcaaaata ctgtggtaga gtaaggcttc
     2101 ataaagttaa tatcttagaa atacaaaaag caattggcga gaattgtaat gaagaagaaa
     2161 ctactattct ttcaagaaga tttatgatga ggatagcaca gagactttct gaaaaaagac
     2221 attgtgatgc tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa actatacaag
     2281 ggcttacttg tactaatgcc gttgtagact taccagtgtt tagaccactt atagcaatgg
     2341 ataaatcaga tatagttgat atagccaaaa aaatagggac ttttgaaaca tctatagtgc
     2401 ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc cgtcactaaa cctagattag
     2461 agaaaataga aaaatctgaa acagcacttg acattgaaaa attagtacag gatgctattg
     2521 acaaaataga ggtagaggat attgagtttt agcatacgag ttatttaatt actaaattga
     2581 atattgagta tattagtagt tctgttgaaa ggatttatgt agacaattta ttaatatgta
     2641 gtatttaaaa ataagtacta taattttatt taatgggaat gattattatg aataaaaagg
     2701 gttttacatt aattgaattg ttggtagtta tatctataat aggaatttta gttatagtag
     2761 ctgttccagc gttatttaga aatatagaaa aaagtaaagc agttacatgt ctttctaata
     2821 gagaaaatat aaagactcaa attgttattg caatggctga ggaatcaagt aaagacaaga
     2881 atgaagtcat aaaagaggta ttagaaaaca aagatggtaa gtactttgaa acagaaccaa
     2941 agtgtaagtc aggtggaata tattcagcaa cgtttgatga tggttatgat ggaataactg
     3001 gaatagaaag cattgcaaaa gtgtatgtta cctgtacaaa acatccagac ggtgttgaaa
     3061 tggctaggga tgtacatcaa agtatgaagg atttgattgc atcatttgca caagaccctt
     3121 ctataatacc aggagcttca aagggcaatg atgattttag aaaatattta ttagacaata
     3181 aatataaaaa tgggtggcct acaattccag atgaatttaa ggcaaaatat ggattaagta
     3241 aggatacact atatatacaa ccatatgcat ataatcctac taaatctgat gctactgtag
     3301 ttgtatttgc aaataataag actggaggta attggtatac ttccctagtt tacgattatg
     3361 atgaaggtag atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg
     3421 ttgacacaga tagtgttaag tctgtaaaaa cagagattca ttctaaagag ggatggggtc
     3481 ctttaaatta atataacatg ttttattaat ctggtattga ttaaactata gaaaagagca
     3541 gtttctatag atgacgaata ccagattttc atttatatat ataattgaga tatattaaaa
     3601 gtgtttatat ttttaagtct tcacatacaa gctaaaaata attataataa tattgtaaag
     3661 aaatttaatt ataagattaa ttttctgaag atttagtatt tgtactaaga aatacataaa
     3721 aaggggaata gatatgaata ataattttca agacaaccaa acaataaact taatacaaag
     3781 tcgtagaagt atacgaaaat ttacgacaga acaaattagt gatgagcaag taaacacatt
     3841 attacactgt gcatttgcag caccttcagg atgtaataaa cagccttggc acataactgt
     3901 tgtgcaagac cagaaacttt taaaagagat tagtgatgat actttatcta ggattcatga
     3961 ggtgagtaat gttgagataa ataaaaactt taagttattt tatagtgctc caaccgtttt
     4021 atttatttct tatgatgaaa gtagttcatg ggctccatat gatataggaa tacttacagg
     4081 gaatataaca acagctgcac aagcactagg gcttggaagt tgtataatag gaatggttag
     4141 aggtttattt acacctgttg aacaagggga tattgaaggt cttgtgagtg tattggataa
     4201 agaagatgta aaagaatcag agtcaataaa gatgaagttt gatacaaata aaaaatacag
     4261 agaactactt gatataccag aagggtattc tgtaccattt ggtattgctg taggtattcc
     4321 agatggaaat ttaccaaatg ctagagaggt agtttataaa gtttccagag tttaggataa
     4381 agtattgcaa gaaacaatca gttacttgaa gaattaaatc agtaatatac aaataaattg
     4441 atggaagagg aagctattta aaaagtagtt tagatttttc gttgtaaaat aattaaataa
     4501 agaataaggt gataagtatt ttatttatta tcttattctt ttttattttt gttttgtaga
     4561 atgataaaaa taaggattaa gtgtaataaa atagaaaaca agtatgttaa ttttaagtat
     4621 atgctttaaa atttatccaa gttaaaaatt taagatatct tttcagtata atggaaaaaa
     4681 tgtcaaaata ttgaaatagg gaaaaaatag tgatataata agaaaaaat
//
LOCUS       DAMJYC010000182         4659 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJYC010000182 DAMJYC010000000
VERSION     DAMJYC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.189"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            68..1237
                     /locus_tag="PC472_003861"
     CDS             68..1237
                     /locus_tag="PC472_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2729132.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1400..1942)
                     /locus_tag="PC472_003862"
     CDS             complement(1400..1942)
                     /locus_tag="PC472_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2729133.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2090..2374)
                     /locus_tag="PC472_003863"
     CDS             complement(2090..2374)
                     /locus_tag="PC472_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2729134.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2437..3201)
                     /locus_tag="PC472_003864"
     CDS             complement(2437..3201)
                     /locus_tag="PC472_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2729135.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3483..3749)
                     /locus_tag="PC472_003865"
     CDS             complement(3483..3749)
                     /locus_tag="PC472_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2729136.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(3791..4600)
                     /locus_tag="PC472_003866"
     CDS             complement(3791..4600)
                     /locus_tag="PC472_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729137.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1585 a          650 c          642 g         1782 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcaggg
       61 tagaaagata aatgaacttt gtaaagatga agttgtagag ttttgcaata gaataaaagg
      121 taaagtggaa agtgttgcaa taagtggagt atttgctcca gtaaataaac aacaagaaga
      181 agaagttgca aaatgggtta gagaaatttt aggggatatt cctatatctt tatcaaatga
      241 aataggtaat ataggtatat tagaaagaga aaatggagct attttaaatt ctgcattgtc
      301 tcaagtaggt aaagctgtat ctttaggatt tgaaaaagca cttaaagact taggtataga
      361 tgctcaaata tatttcagtc aaaatgatgg aacattaatg aatttagagt atacaatgaa
      421 atacccaatt tttacaattg gatgtggaat aacaaattct ataagaggag cttccttctt
      481 atcaaaagta aaaaatgcaa tagtattaga tgttggtgga actacaagcg acttgggtgt
      541 actatataat ggattcccta gagaatcatc tattccagtt acaattggag gagtacatac
      601 aaactttaga atgccagatg ttttaactat tggtcttgct ggtggtacat gtgtaaaagg
      661 cgaaaaagat aatataaaga taggtcctga aagtgtggga tatagaataa ctgaagatgc
      721 aataatattt ggtggagaca ctctaacttt atcagatgtt gttacaaaat tagatatggc
      781 gtttgaagat agaaaaagca atgttgaaaa cttagataaa gagtattgta atgaggtata
      841 taagaaggtt attttaaaat tagaagatgc tgtagaccaa atgaagacta gtcaagatga
      901 tgttgaattg gttttaactg gtggaggaag tataattgtt cctgaaaaac taaaaggcat
      961 aagcaatgtt ataagacctc ctcattatac tgctgcaaat gctattggtg cagctttagg
     1021 acaaataagt ggagatgtag aaaaagtata cagtttagat aaaatgtcga gagaagatgc
     1081 catagaagat gcaaaaaaag aggctgtaaa taaagctcta aaagctggtg ctaaagaaga
     1141 tactatagaa gtattaaata tagaagatgt tccacttgca taccttcctg gtaatgcact
     1201 tcttataaaa gtaaaagttg ttggtgattt aatttaaaga ataaatataa gtatttccag
     1261 aattatataa atatttctgg actcattttt aaacttattt tatcaataaa aacagcataa
     1321 cttttgacta tattcttata agtttccgaa cagccgatat atacctattt tttgaggttt
     1381 atcggctgtt tgtcatcttt tatcgcattc caactatttt tcttaccgta tttgctgtac
     1441 gaattgtaat cttatcactt acatttgaac tggcagtctt tgaccaccaa tttgtctttt
     1501 tataatcttt acgactaaaa gaccaaaaga tagcattttt ataatgatat actttttcat
     1561 actcttcttt aggatttcca acctcatcat aaaactcgtc ataagataag tcaggaaaca
     1621 taaaaataag attatcatat atttctttgt tgttatctcc ccaccaatta ggagcattat
     1681 ttagtatatc ttttaactcc tcttgtgaaa taataaaaac tggtatttcc aattcaaact
     1741 gtttttttat cattaatctt attgtatttg agataacttt gctgtcatct acatcactta
     1801 aaaagacaac attaccacta ttaaggtatg tagaaacttc tgaataacca agttctacaa
     1861 agcttgcttt taattcagac ataattatct tgttcttacc acttatattg atacctctta
     1921 ataatgcgat ataacttttc atataataat ttcacctcta aacattcaca tttttctatg
     1981 tagtacaaac ttgaatttgc atcttttata attctttcaa aacttcttca tatgtaattt
     2041 ttttaatata aaaaaatcgg tgcttaattt gtaccgattt ttgtttttcc tagtaaagaa
     2101 ctcgtttttt tgcttctgct atagctctag cagcactttt tgcttgcatt cttttttctt
     2161 cttctttttt aaattcattt attgtttcat ttagattctt atcatgttca tttttttctt
     2221 tatcttcgtt ttctctgtat actaatatat aatctattat tgattcgttt attatttgag
     2281 atatactttt attttcatta attcctattc tcttaacttc cttaagtaga gtctcatcaa
     2341 tatgaaccgt tgtttgcttt gtattttttg ccaccttaat cacctcttaa aaaaaattct
     2401 aacattaaaa ttttttttag tcaataaatt ttaatattaa aattctttaa atgttttatt
     2461 tgattttcta aaattttcta ttgtatttat catcttattt tttatatttt cacctagtac
     2521 agaagacttt agtctaggat tttcgatatg gtcataaatt ggttctaaaa attcgacttc
     2581 gacaggaaca gagttaattt taccaatagg ttggtatcct tcataagtat cttttgaatt
     2641 ttttattact agtggaacaa tagggcattt tgctttatat gctatcttta aagctccact
     2701 tctaaactcc gaaaccaatg aatttggttc tttaatccat gttaaatctc cttctggaaa
     2761 cactgccata ctttgacctg taagtatatt ttgagaggct tgggctatac tctttatacc
     2821 ttcacgattg ttttttctgt ttacatatac acatcttagt aattttgccc attctttaaa
     2881 tataggtata tttctccaaa ctggttcatc tgcaattaca catccaattg gtctttctac
     2941 acttgctgtt agtatgaagc tgtcaagcat actagaatga tttacaacaa atagaagtgg
     3001 ttcttttggc agtgtttcct taccaattat attaagctct atgttcacta tatctaaaga
     3061 ttttttagct tgtttttgca taaattcaaa tttttccttt aagctaaatt tatcaggatt
     3121 ctttttaatt ttaataagcc taggtataga agatgtaaaa cctataagtt gatatgtagc
     3181 aaattttaag taattattca taaaattatc tccttaaaat aaaattattt attactgtaa
     3241 agattttaac ataaaataaa atttaagtct attttaattc aattttgtaa catatatttt
     3301 aaatagacag agattgagat tttatatttc caattttagt aatcagattt tatcaataaa
     3361 ttactattct attaatttaa ttaaataacc tttaaattta attataaaaa ctgttgcaga
     3421 aatttctaca acagtttaaa aaatttacct tacaaaaact tattttataa caagagagta
     3481 tattataagg ctagtacagc ccattttttg cactgagaaa ttcttttgcc atcatcacct
     3541 tgttctttgt catatgcaaa ttgattaagc atttcacatg gaaatgtatc acatagacca
     3601 caatgtgtaa ggtttttatc ttcacaacaa gattttacag gacattgttc accccaaaat
     3661 ggtttctcaa tctctgtaca acctttacaa ttgacttttt ccttgtatcc acattcataa
     3721 cataaaattc cacatcttga ttctatcatt ttaagtcact ccttaaatat aaattttata
     3781 tgattgtata tcaaaattta tacttttcat tgtaaaaatc ggacaaattt tttatatagg
     3841 ttgttggatt aacaccacaa atagatttaa aatcatttat aaaatgagat tggtcgtaat
     3901 aaccaatatc ttgtgctaaa gttgcaaagg gtattttatt attttgtatt tcttgtaata
     3961 caaggtttat acgaagtaat ctaaggtatg atttaacact cataccaaga gaattattaa
     4021 aaattctatt taaatgtcgt tcactataac aactaatttg tgaaatgttt ttaacagaca
     4081 taatagagtt ctgttttcta gcattttcaa gaattggtat agtcatatct actatatttg
     4141 atttcaataa gtatgataag aataacatat ctaattgttc tactaattcc tttatagtac
     4201 ttgttctttc aaaaatggaa ttaacctcta agctaaataa tgtgttaaaa tcttcaagtg
     4261 aaatttttaa atctgtagat tctctttgag aaagacctgt caaataatat actccacctg
     4321 ggcgaaactc aacgaatact ctaaaaagta cattttctat atcatttttt acaatagtag
     4381 tttttgttgt ggctccccag aatgcgcttt ctataccttt tttgtcaaat ttaaatatca
     4441 tacatccact agcatcaggt attaatgtaa gattttcttt aatacttgta tctggaatag
     4501 atattgtata gtgtgctata tactgtctta acaatttatg tggcaaaata tatgtatact
     4561 gtttgcaggt atttaaaatg ttattgatta ttttattcat aagtatatcc tcctgatata
     4621 taaaaaataa gtatactaat ttatttttta tataataaa
//
LOCUS       DAMJYC010000183         4428 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJYC010000183 DAMJYC010000000
VERSION     DAMJYC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.190"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..748)
                     /locus_tag="PC472_003867"
     CDS             complement(<1..748)
                     /locus_tag="PC472_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2729138.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVVGGGACEKISSITKEKF"
     gene            complement(748..1005)
                     /locus_tag="PC472_003868"
     CDS             complement(748..1005)
                     /locus_tag="PC472_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2729139.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(1025..1255)
                     /locus_tag="PC472_003869"
     CDS             complement(1025..1255)
                     /locus_tag="PC472_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2729140.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            complement(1287..1424)
                     /locus_tag="PC472_003870"
     CDS             complement(1287..1424)
                     /locus_tag="PC472_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2729141.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEIVGEDYI
                     A"
     gene            complement(1425..1724)
                     /locus_tag="PC472_003871"
     CDS             complement(1425..1724)
                     /locus_tag="PC472_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729142.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIEELPT
                     PKYTENKETKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(1721..2953)
                     /locus_tag="PC472_003872"
     CDS             complement(1721..2953)
                     /locus_tag="PC472_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729143.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQS
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETTLSNNYPYLVTNGA
                     IKYIERCSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICVTQE
                     YLFISDNTKLYKINKTTGIEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDM
                     YIMLTKNLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDF
                     RIGGYLHMKNIKFLNDFVIASDAERGIEIDSNKKSALVKYDANLNLIVYSDDSKYDNF
                     DIYNGYIYAIYSLSSSPFVKISLNTLKRIDSYRKLTETYPSAGMFVINDIVFFISSGI
                     FRNILSKKVYSDEKGESL"
     gene            complement(2988..3170)
                     /locus_tag="PC472_003873"
     CDS             complement(2988..3170)
                     /locus_tag="PC472_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729144.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(3170..3466)
                     /locus_tag="PC472_003874"
     CDS             complement(3170..3466)
                     /locus_tag="PC472_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729145.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELHYIDIPYGS
                     IDYIKNRIVGINIETKQPILEEIPVYVSEAEKEKQELENQLLLLTNQEIGGGIL"
     gene            complement(3481..>4428)
                     /locus_tag="PC472_003875"
     CDS             complement(3481..>4428)
                     /locus_tag="PC472_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729146.1"
                     /translation="LITLLQALPFLTVSSYNSTQATFKADHTDPTLLTKIEVFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLN
                     DISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDL
                     MNQLTQANNTITQLNTKYKVASGTVTPFKGDDTKIAYPYLTDRVFKPSAWVKISNLDF
                     KPNIFFADFDYYDTEYKNNYKLFLFACCGVATQRGVDFSSVTSFVRKSSDEYFHANGW
                     LYNNSEGDVYFNNTGVQIPAYSFDSTQRHIYKWYAIKFI"
BASE COUNT         1364 a          787 c          481 g         1796 t
ORIGIN      
        1 taaatttttc tttagtaata gaacttatct tttcacatgc gccacctcct acaacataaa
       61 gattttgcgt ctgacctggc acgtaatctt ttatatcaca tatcagtatt ttcccatcat
      121 tataacccca accaactaca gttgcagaga ttttgtcaac ttctccatca taaacaattg
      181 tatgtttgta catctgttta actccctcat tatttatatt tttatttaat acaccttcta
      241 caattaactt agcaatacct tcatgaccta gtttcttagc tttatcataa tcttctttat
      301 tatcacagaa gaaactttca attaatactg ctgtaggctt tgaactattt aagatatata
      361 atcttttatc taatttagca cctctatttt taaatactgt acctagttta tcacatattc
      421 tagttgcata ctctaagcct ttattactat aatataggac ttctgaacct ttaccttgac
      481 cgttacttgc atttaaatgt aactctataa gtaaatcata tcctccacta ttaactctag
      541 gtattttata agacttttct tcattcttag ttttaaactg cttttctggg catattatta
      601 catctacctt atgcccttct tttctaaatg tatctgctaa tactggtgca agagatttgt
      661 tgtattgata ctcgttaact actccatcag cagaagtaca tgctccactt tttaaaatac
      721 tgtgtcctac tgttatacat attttcatta tttattttcc tccttcaact gtttgtaagt
      781 ttggtttata cctattgata taccccaaca aatcacgcct tgtaagactg caacaggact
      841 tagtcctaac atccaaatag aaaatcctat accaagtatt aataacacta ctggaatata
      901 tttgttatct agttgcttat acttcttaca gcccttacct ataatagaga gagcagctac
      961 taaaattagc aactgctctg gtataaaact tattaaatta tccatctctt atcctcctaa
     1021 ttaattaaaa tattcctctt tgaactgcaa atataaagaa ccctacaagt gttgtaatca
     1081 ttgtaccaat tagccatttg agcatacttg taagtgagtt tagattctca cacaatgctt
     1141 ttaactctgc tttagactct atatttgcta tttttaattc gtctatttct tctccatgtt
     1201 tatttattct tgtttcatgt ctttttaaat ctgcttcgaa aagttcttca ttcatgaaaa
     1261 cctcctaatt tttgaattaa aaaagactat gctatatagt cctctcctac aatttcttta
     1321 tattcacttg ctgttatctt attcttttct actgccgttt taacttgctc tttagtccaa
     1381 ttaccattat tatagaaatc tgttattatc ttgtaccaat tcatttatat caccccattt
     1441 gacattaatt gaaatgttaa atctgctatt gtttgttctg tagaatttac tttgtcttct
     1501 atgctacttt taatatctgt atatctatag aaaacctctt tagtatctat atttataaat
     1561 aactttgttt ctttattttc tgtgtatttt ggtgttggta attcctctat caaaatacct
     1621 tgttttaagt tttcctccga taacaaattt ggttcatagt gtatcatacc aacatatttt
     1681 atattttgtt cttctgtatc cattaaattt cctaaataaa tcataatgat tctcctttct
     1741 catccgaata aacctttttt gacaatatat ttctaaaaat tccactacta ataaagaaaa
     1801 ctatatcatt tattacaaac ataccagcac ttgggtatgt ttcagtaagt tttcggtagc
     1861 tgtcgatacg tttaagagta tttaaactta tttttacaaa aggactacta gaaagcgaat
     1921 atatagcata tatatatcca ttatatatat caaaattgtc atatttgcta tcgtctgaat
     1981 acacaattaa atttaaattt gcgtcatatt ttactaaagc actttttttg ttgctatcaa
     2041 tttctatacc tctttctgca tcagatgcaa taacaaaatc atttaaaaac ttaatgtttt
     2101 tcatatgcaa atatcctcca attctaaaat ctttagcaat ggcaaaatca aaatttattt
     2161 tagataaatg acatgttgta atactacttg aatttgagtg ttctgttgta gcataaatag
     2221 cattcttatt gcaaacaaac ttacctcctt taaaattgta aataccatta gaaactaaat
     2281 ttttagttaa cattatatac atatcagata ttctaatttt atgaagtgta gaagaagtct
     2341 catctccata tactccataa ataaattccc cataagtaca taacttataa taagcacctt
     2401 ctattgactg cacttcaatt cctgttgtct tatttatttt atataattta gtattgtcag
     2461 atataaataa atactcttgt gtaacacaga tacatgagaa gttagcatta gctaaagtaa
     2521 tgtcaaaaac tactgtttca tcaatagcat taattttaat tagatgagtt tctttaatta
     2581 cataaaaata tggttcttca tattcaaaag ttttgaagtt cccactacat ctttcaatat
     2641 attttatagc tccatttgta actaaatacg gatagttatt tgaaagtgtt gtttctctta
     2701 aatctaatcg cccctctttt atatcaattt tattctttat ttcttcccat gtatcgcttg
     2761 tagtaacctc tgcacctttg gagtttaatg ctgttactac attattttta gcattaactc
     2821 cactttgaaa aacctctttt aatgctcctt ctacattatc acttgtaaaa ttattctctg
     2881 tatcttctat agttacattc tttgcttcta atacaagatt tctaacttta ttaactaact
     2941 ctttaaaagt catttagtca ccttctttca ataaaaaaaa gaacctacta cgctgttggt
     3001 tctattcctt ctactactcc actatttttt ataatataat cctctactgc ttttctgtat
     3061 tctgtgttag ttacatcatc aagttgaaac tctctatttt tcaaagggtt taaccctcca
     3121 tttaatattc tttctgctaa tattcttacc acaacattat ttatattcat tataaaattc
     3181 ctcctccgat ttcttgattt gtcaataaaa gtaattgatt ttctaactct tgtttttctt
     3241 tttctgcttc acttacatat actggtattt cttccaaaat tggctgtttt gtttctatat
     3301 ttatacctac aattctattt ttaatataat ctatacttcc atatggaata tcaatataat
     3361 gtaattcagt tattttattg tgtggtaaca catccccaca ggactcacct gtttcaaaaa
     3421 ttattttacc tgtttggtca tatattactc tatttggaac attcatgttt cttctccttc
     3481 ttaaataaat ttaatagcat accatttata aatatgtctt tgagttgagt caaagctata
     3541 agctggaatt tgaacaccag tattattaaa ataaacatct ccttcggaat tattatagag
     3601 ccagccatta gcatgaaaat actcatcact actttttcta acaaaagatg taacacttga
     3661 aaaatcaaca cctctttgtg tagcaacacc acaacaagcg aaaaggaaaa gtttataatt
     3721 atttttatat tcagtatcat agtaatcaaa atcagcaaag aaaatattag gcttaaaatc
     3781 taagttacta attttaaccc aagcgctagg tttaaaaact ctatcagtta gatatggata
     3841 agcaatttta gtgtcatcac ccttaaaagg agtaactgtg ccacttgcga ctttatattt
     3901 tgtatttaac tgtgttatgg tattattagc ttgtgttaat tgattcatca aatcctgcac
     3961 actagcgtca gaactatcaa aacttgtttt tattttctct gataactcga ctaatgtatt
     4021 atttaaactt gcctctatat tctttaatgc taaagtattt ataatgcttg ttttcccaac
     4081 tttaaatcct gcattaacct caactaattt tgttgatata tcatttaaat ttacattttc
     4141 gggcagtggc attatattct tacttatact taaaactttt tcagcagttg cattattgct
     4201 gtctgtaaca actattttaa gcgtgtgcag tgcattatct tctaatgtat agttaattgt
     4261 tttctctgtt gttaaatctg ttgttatagt ttcttttagt acatcatcta taaatacttc
     4321 tatcttagtt aatagtgtag ggtctgtgtg gtcagcttta aatgttgctt gtgtggagtt
     4381 ataagaggat acggttaaaa aaggtaatgc ttgtagtaat gttattag
//
LOCUS       DAMJYC010000184         4424 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJYC010000184 DAMJYC010000000
VERSION     DAMJYC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.191"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            165..1640
                     /locus_tag="PC472_003876"
     CDS             165..1640
                     /locus_tag="PC472_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2729147.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1727..2140
                     /gene="speD"
                     /locus_tag="PC472_003877"
     CDS             1727..2140
                     /gene="speD"
                     /locus_tag="PC472_003877"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2729148.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2170..3021
                     /gene="speE"
                     /locus_tag="PC472_003878"
     CDS             2170..3021
                     /gene="speE"
                     /locus_tag="PC472_003878"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2729149.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3011..3889
                     /gene="speB"
                     /locus_tag="PC472_003879"
     CDS             3011..3889
                     /gene="speB"
                     /locus_tag="PC472_003879"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2729150.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4069..4269)
                     /locus_tag="PC472_003880"
     CDS             complement(4069..4269)
                     /locus_tag="PC472_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2729151.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1747 a          496 c          758 g         1423 t
ORIGIN      
        1 taatgtatat gtttttgagt aacttcaaaa aaacacttgt aaaaatgctg ggatgttaat
       61 ataataaata tcatgagttt aataaaagta aaaataaaaa atagaaataa taaataataa
      121 atattaaaaa tatttgaata tagagaggag atgaatttga aatcatggat agcgagtttg
      181 caaatgcagt tgaattaagc tatgagtgtg ctccattatt agagtcgtta aaggaatact
      241 cagagaaaga tatagcttgt tttgatgtgc ctggacatgt aaaaaataga ggtgtagcta
      301 tattaaataa atatctaggt gaaagtctca tgaaaatgga tataaattca tcaccaacta
      361 tggataatgt atctgcgcca aatgggatta taaaaaatgc acaagatttg ctagcacaag
      421 catatatggc tgatgaggca ttctttataa cgaatggcac aactcaagca atacatgcaa
      481 tgattttgag tgttataaaa ccaggtgaaa aagtattact tccaagaaat atacataaat
      541 cagtaataaa tgcgttaata ctttgtggag gaataccaat atttatacaa ccagaatttg
      601 atgaaaaact aggtataagt ttaaatatta ctcttgaaaa agttagaaca gagatagaaa
      661 aagattgtaa tataaaagca ttatttttcc ttaatccaac ttactatgga gtttgtgcag
      721 accttgagag tattatagaa ctatgccata aaaataatgt tttagtgtta gttgatgaag
      781 ctcatggtgc acattttcca tttcatttag atttacctcc ttctgcaata agtttgggag
      841 ctgatatggt agctgtaagt atacataaaa ctggaggagc attgacacaa tcttcagcac
      901 tcttattgaa tagagataat gtaagttttg aaaaggttct tcaatcaatc aatatgctac
      961 aatcaacatc agcatcttat ttgcttatgg caagtataga tggtgctaga gttaatctag
     1021 ttgagaatgg tgaaaagcaa ctgtcaaaag cattaaatct atctaggtat gcaaaatcta
     1081 aattaaacaa aattgatgga ataaaagttt tatctacaga aatattgaaa caaaaaggtg
     1141 ttaaatttat agatgaaaca aaattgtgta taaatgtaaa agaattaaat ttgactggat
     1201 ttgaagtata cgatttatta tataaaaatt tttcaattca agtagaattg ggagattcat
     1261 ataatatact ggcacttgtg tccattggaa caaataaatc agatatagat agattagtaa
     1321 aagcactttc tataattgca aagatttata gaaaagaatc aacattaaat gaatttaata
     1381 tggttcaaat aaatccaatt attaaactta atccaagaga agctttttat gcaccaaagg
     1441 aaagtgtaga aatcaattca tgtatagata gaatttgtgg agaatcaata atggcatatc
     1501 caccaggtat accgattatt gctccaggag aattgataac agaagaaatt atggagtata
     1561 ttattttctt aaaaaatagc aatgcatatc ttacagatgt acaagataaa aacttagata
     1621 gaatattagt aataaaataa attactttat tttgcaaggg caagtatttg ttaagtagag
     1681 tttatcaaat agaacttatt aaaagtataa aggagttggg gttgaaatga aatttgaaac
     1741 actaggaaga catatactag tagaatacta taattgtgat gaaaacgtac ttaaagaccc
     1801 acgtcttata gaaaatttta tgaatgattc agcattaaat gcaaaagcga caattgtgga
     1861 ttctgtattc caccatttta atccttgggg ggtttcagga gctgttataa ttgctgaatc
     1921 tcatttgaca atacatacat ggcctgaata tggctatgca gcagcagatt tttttacttg
     1981 tggtgatata gacccatgga aaagttttga attattagag caacttttaa agtctgaaag
     2041 aagcgagtct acagaaattc caagaggctt aactacaaaa attaaaaaat attcaaaaaa
     2101 agatttaggt aagattactc ataaaccaga agcagtataa aacatacaag agaaagtgag
     2161 gattattata tggaactttg gtatacagaa gaatggacag aaaatgtaag attttctata
     2221 aaagtaaata aacatctatt tgaaggaaag tctcaatttc aaaggataga tgtgtttgat
     2281 agtgatgaat ttggaaaatt tctaacaata gatggattaa tgatggtaac atataaagat
     2341 gaatttatat accatgagat gataactcat gttcctatgg ctacaaattt aaacataaag
     2401 aaagtattag tcataggtgg aggtgatgga ggtacagtaa gagaattaag ccgttatcct
     2461 caaatagaaa agattgacat ggttgaaata gataaaatgg ttgtagatgt atctaaggag
     2521 tatatggata tatgctcatg taaattagat gataaaagag tgagtttata ttttgaagat
     2581 ggtgttaatt ttgttaagtg tgcacatgat aaatcatatg acctgataat agtagattca
     2641 actgacccaa taggaccagg tgaagggctt ttctcaactg atttttataa ggattgttat
     2701 agaatactga ctgatgatgg aattttagta aatcaaagtg aaagtccgta ttttgacttt
     2761 aatgctaaag agatgaaaag agctaataaa aaattaaaac agatatttcc aatatcagaa
     2821 gtttatcaag cacatatacc aacatatcca tctggtcact ggttgtttgg atttgcttca
     2881 aaaaaactta atccagtaaa aaatcaagat agaaatggat gggagaaatt aagtttaaaa
     2941 actaaatatt ataatagtga tatacattta ggttcgttta tgttacctca atacgtgaag
     3001 gagatgctag atgaagaata atttctatca tatgaacact tttatgagta tggacaaaaa
     3061 ttatgaagaa tctaatctta tagtatttgg tgttggattt gatggaacta cttctaacag
     3121 acctggggct agatttgcaa gtagctctat gagaaaagaa ttttacggtc ttgagacata
     3181 tagtcctttt ttagatttgg atttagagga ttataatata tgtgattatg gagatttaga
     3241 aattagtgtt ggaagtacag aacaagtctt aaaagaaatc tatcaagaga catataagat
     3301 tgttagagat tcaaaggtac cctttatgat tggaggagag catttagtta cattaccagc
     3361 ctttaaagca gtacatgaaa agtacaatga tatatatgta attcattttg atgcccatac
     3421 tgatttgagg gaagaatata ataatagtaa aaattctcat gcaacagtaa ttaaaagaat
     3481 atgggatatt gtaggtgata ataaaatatt tcaatttggt ataaggtctg ggacaaaaga
     3541 agaatttaaa tttgctacag aagaaaaaca cacatacatg gaaataggag gaatagatac
     3601 gtttgaaaat atagttaata tgctaaatgg aaagaatatt tatctaacta tagatttaga
     3661 tgtattggat gcatctgtgt ttccaggaac aggtacacca gaacctggtg gtgtaaatta
     3721 tagagagttt caagagattt ttaagattat aaaaaactct aatataaata tagttggttg
     3781 tgacattgta gagttaagcc ctgattacga tacaacaggt gtatcgacag ttatagcttg
     3841 taaaatctta agagagttat gcttaataat atctgataaa attaaataga gtattttata
     3901 taaatagtat atattataat ataagaagtt attttaaaaa agttagttta agtttgacgc
     3961 ttttctaaag taacttctta tttttgtaat taaaaataaa tataataaac aacaaaaaag
     4021 agccctcgat agctcctttt ctaaaatata tatatttaaa ttaaataatt atattctatt
     4081 tacgttttca gcttgaggtc ctctattacc ttgagttata tcaaagctaa ccttttcgcc
     4141 ttcttctaaa gttttgtatc catcattttg tatagctgag aaatgtacga aaacatcatc
     4201 ttctccctct acagatatga acccaaatcc tttttcattg ttaaaccatt ttactattcc
     4261 gtttttcatt ataagaaaac ctccgcatta ttaaaaaata caaactaaaa aaataagtct
     4321 gttctattaa tataacataa attttaaata aaagcaaata aaatttttaa aaaaattaat
     4381 tacttttaga atttaataaa aattgttaaa ttatacagtg agaa
//
LOCUS       DAMJYC010000185         4380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJYC010000185 DAMJYC010000000
VERSION     DAMJYC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.192"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(61..606)
                     /locus_tag="PC472_003881"
     CDS             complement(61..606)
                     /locus_tag="PC472_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2729152.1"
                     /translation="MKKFVCTVCGYIHEGDTAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(724..2547)
                     /locus_tag="PC472_003882"
     CDS             complement(724..2547)
                     /locus_tag="PC472_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729153.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDINTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMVSNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTILSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2560..4335)
                     /locus_tag="PC472_003883"
     CDS             complement(2560..4335)
                     /locus_tag="PC472_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729154.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1415 a          793 c          390 g         1782 t
ORIGIN      
        1 ctcactcata actttaaaaa ttataagtga gatactccta aaatctactt tatattaaat
       61 ttattttcca aaatatctat ttaataatcc taagaatgct ttaccatgtc tagcttcatc
      121 tttacacatt tcatgtactg tatcatgtat agcatctaat cctaattctt ttgctttctt
      181 agctaatttt aattttccat cagttgcacc atactcagca tcaactctaa ctcttaagtt
      241 ttcttttgta tctgcaacta caacttctcc aagaagttct gcaaatttag cagcatgttc
      301 agcttcttca agagctattc tcttataagc ttctcctact tctggataac cttctctatc
      361 agcttgtcta ctcattgcta aatacattcc tacttctgta cactcaccaa caaagttagc
      421 tcttaatcct tcgattattt cttcatctac accttgagct actcctattc tgtgctcatc
      481 agcccaattt ttttcgcctt tcatttcttc aaatttatca gctccaactt tacatactgg
      541 acattgtgct ggtgcagtat ctccttcatg tatatatcca catactgtac aaacaaattt
      601 cttcataatt actttcctcc taaattttat atatcaaatt aaatttttta tttattaagt
      661 tagtctatat atacccgcac catatacctt taaacaattt ttttaaaagt tttttctaaa
      721 tacctatttt ccctgtgtca ataatatgtt tctataataa tgtcccttta attttatcaa
      781 ttctttatga tttcctgatt ccacaattct accattttct aaaactatta tattatcaca
      841 atttgatatg gtacttaatc tatgagctat aattatactt gtctttccat aagttaaatt
      901 atttattgct tcttgaattt tagcttctgt tgctatatct atgttacttg tagcctcatc
      961 caaaataagt attggagcat tttttaatac tgctctagca atacttatta attgtttttg
     1021 accttcactt aaatcttcac ccctatccga taatatagta ctatatcctt cacgcatttt
     1081 aactataaat ttatgtgcat ttgctatttt actagcatat ataacttctt catcagtagc
     1141 ctccagattt ccatacctta tattttccat aactgtatca ttgaatatat aaccttcttg
     1201 tggaactact ccaaaacaag ttctcaaata gtttctgtta atagagtata tatccttacc
     1261 atctaaaaat atacttcctt catctatatg ataaaaattt gtaattagat ttataatagt
     1321 tgtttttcca cttcccgtct ctcctatgat ggcatttgat gtgccagctt tgatttttag
     1381 gtttatatta tctaaaattt tcttttttga atcataagaa aaacttacat ctttaagctc
     1441 aatttctccc tttacaaaat gagagtttaa attaccatct ttattatcgt taatctcact
     1501 tttaaacatg ttatgcactt cttgctcttc ttgttcatct ataatttcaa aaactctttc
     1561 acatccagct atggctgaaa gtaatgtatt aaatagattt gctaattcat ttaaaggtct
     1621 tgtaaattgt cttacataac ttaaaaaact agctataacc cctatggtta taatatcttt
     1681 agatgcaaga atgcctccta taatacatat agttgcaaat cctatattac taatcacatt
     1741 cataataggc attaaaagag aagtgtaaat ttgcgattta agggaaatat ttagaagtct
     1801 atcattgact tctttaaaat catttacaga ttttctttca taattaaaag attttactac
     1861 atttatgttt gataccattt cttcaatatg tgcatttagt tttcccaact caacttgttg
     1921 ttgcttaaaa aagactctag ttctctttgc aataaaatta ctcacaaaca ttactatcgg
     1981 tattaatatt acactgcaaa aggttaaaat tgggcttaat acaaacatca taataaaagt
     2041 catcaatatg tttaagattc ctgtaattaa ctgtactaat gaatttgata ttcctgtact
     2101 gatattatca acatcatttg tcactctact cataatatct ccagtagaac ttttatcaaa
     2161 aaaggttatt ggtagatttt taaattttaa aaataaatta cttctcattt ctctgactat
     2221 actttgagat aatctagctg tacatatccc cttagacagt ttaactgaaa actcaattat
     2281 ataaaatgaa agtaaaataa ttattccttt taagaaaatg gattttgaat aagtattgat
     2341 atctattatg tcaactactt ttccaatagt atatggtatt acagttgata taattgaatc
     2401 aattattact agcaaaaaaa taaatctagt aatcttctta tcttttttaa agtacataaa
     2461 aattctttta aacgtttttt taaaatcttt aggcttttct tttttcttta atctactgac
     2521 cttctgattt ccagtactta aattcaatat acttcctcct taatttacat cttcacttac
     2581 taaattctgc aacgcaaaaa tttttttata aattggagaa ctaaccagta aatcttcatg
     2641 tgtagcaaat ccaaccacct caccctcatc caagactaaa atcttatcac agtcaattat
     2701 agaactaatc ttttgtgaaa tagttattac agtacatttc ttactttcct tttctcttaa
     2761 attacttata tactctttta tattctttct tataattgtc tctgtattaa tatccattgc
     2821 actaaaacaa tcatcaagaa ttaatatttg tggttttttg gctatagctc tagctatagt
     2881 aattctttgt ctctgtcctc ctgataaatt tttacccctt tggaataaaa cttcatcata
     2941 agtattttcg agttttgtta taaattgatt tgcatttgct atttcacatg attttacaac
     3001 atcctctttg catatatctc tatttcccat aactacattc tccaatatac tttttccaaa
     3061 cacactacta ttttgaaata ctacacctat tctttccctt aaactttctt cagaaatact
     3121 gtatatacta cgtcctccaa tcttaatatc acctttatca attatatata atttttgaat
     3181 taaatttact aaagaagtct ttcctgaacc agtttgacca atcacaccaa tattttgacc
     3241 ttcttctatt ttaaaggata cgttttttaa tattttttca tctgtatttt tgttatacga
     3301 aaaatctaca ttttcaaaaa ttatatcaca gtttacaaac tttaaatctt tttctccaac
     3361 gtctaaactt tcttctctct caattacttc ttcattctca tcaaccaaaa cctcacttat
     3421 tctctcataa gatgcttttg ccctaacaaa aacattaaaa acatgtgaaa tcattataag
     3481 agatgttaat attctagtca tatagtttac ataagcaact ataatcccaa ttttaatttc
     3541 tcctgaataa attaaatgcc ttccaattag taatattatt attatggaaa cattaatgat
     3601 tattgtaatt gtcggtctaa aaaatgacat catagttgta gcttttctac tatttttaaa
     3661 aagttcatca tttaactctt caaagctttg gttctcataa tcgtatctac aaaatagttt
     3721 aactactttt attccactta aaaactgtct taattttgaa tttactttat ctgtactttc
     3781 ttgtacattt ttaaataatc tataccctat cttcatatta agcaatatta taataaatat
     3841 aattaaaact gcaattagaa taattattga catctttaaa tttaatttta tagctataat
     3901 taaacttcct atacacatta ttggtgactt tataaaaact ctcatcattc cattaacgaa
     3961 ttgttgcatt tgattgatat cattagtaat tcttgttatt aatgaagctc taccaaattt
     4021 atctatttgt tttaatgagt atgtatttat cttttcaaat acatcctgtc ttaaatcata
     4081 agaaaaatgc tgagaaactc ttgccgagat tacacttctt attattgcaa atattgcacc
     4141 taaaaaagtt atacctatca ttatagctcc tgttgatagt atgtagtcta agttcttatt
     4201 tgaaacccct atatctatta tttttgccat tatagttggt tgagctaaat cacaaaaagc
     4261 ttccaatgtt aaaaaaaata ctgctgttag aaataatact atatattttt ttatgtattt
     4321 tttaaaatac ctcattcaat catcccttca tagaattttc tcattaccaa aagtatatca
//
LOCUS       DAMJYC010000186         4278 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJYC010000186 DAMJYC010000000
VERSION     DAMJYC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.193"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(226..609)
                     /locus_tag="PC472_003884"
                     /pseudo
     CDS             complement(226..609)
                     /locus_tag="PC472_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      835..932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            987..1163
                     /locus_tag="PC472_003885"
     CDS             987..1163
                     /locus_tag="PC472_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729155.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1389..1589
                     /locus_tag="PC472_003886"
     CDS             1389..1589
                     /locus_tag="PC472_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2729156.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   1953..2243
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2019..2047
                     /rpt_unit_seq="ctttacataccacatagttgatataaaac"
     gene            2470..2652
                     /locus_tag="PC472_003887"
     CDS             2470..2652
                     /locus_tag="PC472_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2729157.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            2723..3567
                     /locus_tag="PC472_003888"
                     /pseudo
     CDS             2723..3567
                     /locus_tag="PC472_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3791..3955)
                     /locus_tag="PC472_003889"
     CDS             complement(3791..3955)
                     /locus_tag="PC472_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729158.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1625 a          538 c          490 g         1625 t
ORIGIN      
        1 agtcaatata aaagacagta atttatctat agtgttactt atattttata tatatggcta
       61 tttttcaaca ttttacaata tataacattt atttttttaa cttttgtcag taaaatgtca
      121 gcaaaacact ttatccacaa tttttatata agttttccac aacttatcac caatattatc
      181 cacataaaaa aggtagcact tatattatta gctacctttt atttattaat ctttatattt
      241 ccagcttttt atccaacttc ctatttctat tattttttct tcagttattt cttctccatc
      301 atgtaatttt gaaattaatt ctgataaagg attgttatgt aaacctctaa aaactctttt
      361 tccctctatt ttgagatatt ctctctcttt tattaatact gtatagtgta catgcctccc
      421 aattctctat gatattaaaa aacctttttt ggttaatcta gctaaaagta taattgtagt
      481 tgtatgcttc caaccatatg ttttttctat ttcatctgca acatcttttg atattacagt
      541 tttataacca gtatgccata tgcatttcat aatttttaat tctgtatctg gtaatttttt
      601 atttagcata atatattcct tctttcaatt tatgtcactt cttcaaattt atctagctta
      661 aattataata ggagcttttt agaacaaaaa ctatatttaa tttttaccag gtactctacg
      721 tgtttagtct gtttttaagt gtaaaataat gttcatatct ataaatttat ttaactttca
      781 aacaatcctg ttgaataata actaattcta ttgtattatt ttagttgtag aataaaacta
      841 aatcggcaaa actagagaaa tttagtgacg caaagctata gggactaaga cttatataaa
      901 tatcttatga gttatgtcag ccagttgcca aagagatagt gttctttttg tttttatgaa
      961 agttttttag ggggataata atttatatgt ttggaaataa aatggataaa tgtacacatg
     1021 tattgactgc ttatattagc agttcgtatg attattgtaa ttttttagat acacagttag
     1081 atgattttat attagagtac ggagaaaatg tagtagagtc ttgtttacac caagtaatgg
     1141 tattggtaag taagtataat tagatagaca ttgatttcta gaaaaccctt tttagtttta
     1201 tctacattta tattaatata tagcaatgta aaacgtttaa atatattttt aatattaaga
     1261 ataattaaaa tgttaactaa aaaatataat taagtagaca tttctattaa tctatgatac
     1321 aatagtattt gtggtaatac acaattaaat caagtatatc ttgatatagg ttaataggag
     1381 tatttttaat gaataatgga atagtgaaat ggtttaataa tgaaaaagga ttcggtttta
     1441 tatcaatgga aggtagagat gatgtgtttg ctcatttctc agctatacaa acttcaggat
     1501 ttaaatcatt agaagaaggt caacaagtaa gctttgatat agttaaaggt gctagaggtc
     1561 ctcaagcaga aaatataact atattataat atatagattt ttcgggaaag actcttttta
     1621 gagtcttttt ttattgttaa tatatgtaag ataatcattg gtgaagtaaa taaggtatat
     1681 tgagtaatgt tcttgatagt ttatatgcta atgaatttca aaaaaataag cactcctata
     1741 aaaagagtgc tcttaatgta aatataaacc ttcattggtc cagcctaata ccccgtattc
     1801 tccaagttct aactcttttt tatatacatt atcactttca gccatatcta acacaatcat
     1861 ttcttcttta gaatcaattt gtaaaatatg aggactgtgt gttgtgacaa ttatttgtgt
     1921 ttttgggaga attgttttta atgtaataaa aattttacat accacatagt tgatataaaa
     1981 cttaagacta gcagactcat aagcaattag ttgcctctct ttacatacca catagttgat
     2041 ataaaacttt aataataagg agagaaaaaa tgaatattta tgactttaca taccacatag
     2101 ttgatataaa actaattgga gtccaatcta atttaataga aggaatactc tttacatacc
     2161 acatagttga tataaaaccc tattcttata tttcaaatcc tttgagtgct tatgctttac
     2221 ataccacata gttgatataa aaccccaaaa taaacttagc atctccaatg tttacacata
     2281 tacaatgctc ttaaaattgc agtgaacgcg caatagtgca attgataata cttattatat
     2341 attctcaatg ccttatattt caactgctaa acctcaattt gtcacaaata tcgctcactg
     2401 caaaatttct atatttttat tatatcataa catataatta atattttcaa ttaattttta
     2461 catttaatta tgtttaaatt ctataataat gcaactttaa tgtttctttc tggaactaat
     2521 ataaaaacta tttcagaaag attaggtcat gcagatataa aaataactat gaatagctac
     2581 tctcagtttt tagaagagat ggacagagaa gcatttgaaa atttgagcaa aatactattt
     2641 attatatttt aaagaagatg acttattcaa tatttgatgc tagaatataa ttaataaaaa
     2701 tttaattata ggtgattgta ttatgaatat tattaaaagc ataactttac tcttaacatt
     2761 attaattgtg atattaatga caggctgttc cccaagagaa agtgctagtg aagttgcaaa
     2821 aaaatatatt gataagatga actctggtga ctatgaaaca gcatatagtt tattaagtaa
     2881 agatagtaaa aaaaaaatta gtatagaaaa tcttgaagaa tttcaaaata ttttagttag
     2941 tacaaaaaga ataattagtt ataaaatagg taaagaaaaa atcataaaaa aatataaaca
     3001 tgatggaaaa gaatataaaa atgttgttaa attagaagaa acttttaata taaaaaacct
     3061 tctttataaa aaagatgatt ctctaaaagt aaatagatat tttgtagttg aaaataacaa
     3121 atacaagcta ttattgtatg aaagtttcaa aaaagaattt ggtattaata gtatggacct
     3181 tgctcaactt aagcttaatg attttggact aaaatacttt aaagaagttg accttatact
     3241 aaaaaaagct attgctatta atcctactga ttcaaagctt tattatgccc aagcatgtaa
     3301 ttatatgaat tttgatggat ttcctggtta ttcagataca acatataatg ctttagattc
     3361 aattaataaa tctttaaagt atttagataa aaatgattct gactacaaaa aaatgcaagt
     3421 aatatatata acttaaaagg agttattctt atgtttcaca aaagatttga tgaagcacaa
     3481 caatatttaa ataaagcctt atactttaac aaagataatg aagacccaaa atataatcta
     3541 aatacaataa aaaaacatct aaactagata tttaattttt gaatttataa aatatttttt
     3601 tatgtataat tcagcactga atcataatct ctactaaact aattacaagt ttaaattata
     3661 aagacttatt tctatataaa tattactcta taacaataag tttcttattt aaattttaaa
     3721 atattatcta aaaatcataa aaaagaaggt ataaagagct accctcaagc tcaatatacc
     3781 ttataaatat ctaaacttca ttttttacta atttttctat atctttgata atatgttctt
     3841 tagatttttt tccagctatg gcatcaaaaa ttgcaacagc ctttttctct ccaaactttt
     3901 ctgctacatc attaaagtct aaaaatctaa cactttcttt tggtttacca aacatataac
     3961 acctctcata catttttata tatttattat aacttctata gacttatttt tcaccaataa
     4021 acgttagaca aattagtccc tttatttttt taaactagct ttaccttcaa aattcttaac
     4081 aaaaacagtt tattctactc tataaataaa aatagacaat ttaatttgta ggaaactgat
     4141 aatagaatac agtaaataaa actttgttta atctatctga aaaagttatc tattaaaact
     4201 gctatgaatt tataaagaat attctagttc ataaaaatac aaatatgtaa atataatcac
     4261 cttaatatta atttattt
//
LOCUS       DAMJYC010000187         4269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJYC010000187 DAMJYC010000000
VERSION     DAMJYC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.194"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            269..1888
                     /locus_tag="PC472_003890"
     CDS             269..1888
                     /locus_tag="PC472_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2729159.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTTRQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2262..2456
                     /locus_tag="PC472_003891"
     CDS             2262..2456
                     /locus_tag="PC472_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2729160.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(3015..3338)
                     /locus_tag="PC472_003892"
     CDS             complement(3015..3338)
                     /locus_tag="PC472_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729161.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3383..3637)
                     /locus_tag="PC472_003893"
     CDS             complement(3383..3637)
                     /locus_tag="PC472_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729162.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3901..4182)
                     /locus_tag="PC472_003894"
     CDS             complement(3901..4182)
                     /locus_tag="PC472_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729163.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         1554 a          559 c          496 g         1660 t
ORIGIN      
        1 tttacaatta tagttaattc tttgttaaat tgattgtaca taaaatacta aaaatctaag
       61 ttcaagtatt taaattgcta tatatcataa attttctaaa attaacaaca acttaaaaat
      121 ttaacattct cttaacgtat acttaacaaa acattaatat ttttattgac aaaatttgtt
      181 attctatatt atggaaacaa taaattttag ttaataaagt ttataattca gataaaaaaa
      241 ttattaattt cttaagggag gaaatatcgt gaatatagta attagcctta tcggtggact
      301 tggacttttt ctttacggaa tgagtttaat gggagaaggt ttacaaaaat cagcaggaga
      361 caaattaaaa aaaattattg aactcttaac aagtaatgtt gttatgggtg tactggtggg
      421 tacagtcgtt acaggtatta tacaaagttc aagtgctact actgttatgg ttgtaggatt
      481 tgtaaatgca ggtataatga atctaagtca agctattggt gtaataatgg gggcaaatat
      541 aggtactaca gttactgctc aattagtttc atttaatctt gaaggaattg caccaattgc
      601 tttaggtata ggtattttat tctatttatt tacatcaaat caaaaaacaa agcatttatc
      661 tgagatactt ataggtttcg gtatattatt tacaggaatg gagtttatga aagatgcagt
      721 agctccactt gctgaatata aagcattcac tgatgcactt ttatatttta gtaaaaatcc
      781 tgtattaggt atattggcag gatttgccat aactggtatt gttcaaagtt caagtgcatc
      841 tatgggtatg ttgatagcac tagcttctca aggaatactt cctctatcat ctgctttacc
      901 tattctttat ggagataata taggtacttg tgttacttca cttttatcaa gtgttggtgc
      961 aagtagaaat gctcgacgtg cagctgtaat gcatttaagt tttaatgtaa ttggtacaat
     1021 aatatttatg cttgttttaa ataaacctat atcagccata gttactcatt ttgacccaac
     1081 tgatacaact agacaaatag caaatgcaca tactttattt aatcttacaa atgttataat
     1141 attattacca ttttctaaat acatagttaa attagcaaat agacttatac ctattaaaga
     1201 aactgaatca gaaatagtaa ataatactaa gtacttagat gaaaggatgt ttagcactcc
     1261 atctatagca cttggtaata ctgtacaaga agttgtaaga atgggtcata aagctacaaa
     1321 ctctttggaa cattcaattg caggattttt aaataagagc aatgaagata ttaataaagc
     1381 ttttgagtca gaaaaagttg tcaataagtt acagaaagat atattgaact atttgttaaa
     1441 actttctaaa gaacctctta gagatgatga acgctttaga acagatttgc tttttaatac
     1501 tgtaaatgac attgaaagag tctctgacca tgcagaaaat atagctgaat tggccatgag
     1561 tgttaaggaa atgaacatta gtttttctga tagtgcgata agagaaatat acgaaatata
     1621 caataagaca attactaatt ttaaggatgc cttaatagtt ttagatgtta aagattttga
     1681 gttagcaaat aaagttttag aagttgaaaa tgaagtaaat tatttagaaa aaacttttag
     1741 aaattcacat atgattagac taaataatgg aagttgtaca attgatgctg gtgtacttta
     1801 tcttgactta cttactaatc tagaaagaat ttctgaccac tcaacaaata tagtaaaaca
     1861 agtactaaaa cttaaacaaa aaatatagtg attattttta tatataatct taatcaatga
     1921 gaagtaagaa gctatagata atgcttatat catctatggc ttcttatttt ttatgtaata
     1981 ctcaaatttt tatagtattc tcttattatt aaacaatttc tgcctagaat attccatata
     2041 tatatgactt ctttcataaa acttgaactg cccataacta aagtcacaag tatacataac
     2101 tattttttaa tcaattataa ccaatatttg ttttatttat cttcatttta attcttcatt
     2161 caataatgta ataagattat tttttatttg gatactatta tatcatctaa atatgttatt
     2221 atatatttgg atattttcat attaatagga gggattctac aatgataaat tatatggtag
     2281 aaattatata tagtgaaaaa ataagcgaag tagaaaacat gttgaaaatt gaaaatatac
     2341 aatatgaatt tacttctcgt gatggcttta tattttttgg tttttatgat atgacagacg
     2401 catatagatt tgagacttta tgttcagatg aaaaaattcg atttaaaaga atataataga
     2461 taaaaaagta catatttcta aactatttgt ttttcaaatt taggttgtgt tttaatgtag
     2521 tgttgatatt ataaacaatt ttaataataa aaaagtaggg acgttatatt aatatgctcc
     2581 tactttttac tcatacttat taatattatt tatcaaaatt accctacaaa ctggaattta
     2641 tcattctttc ataattttaa actatcatat ttaaaatata taaaattcta catgattttt
     2701 gtataaacat aatatcaacc attatccaat ttaaaaatat tttttagtaa tttccatttc
     2761 attttctctt aacatattaa ttaaactttt atatgtaaat ggtgtattta catattattt
     2821 aatatccaac aattatataa agaaatgtgc tctttgcctt ataaagaatt ataaagccaa
     2881 ttcgttttat tatattattt ttctttatac cttaaaaact ttaccttcaa attcttattt
     2941 atctatatat tataaaaatg ttcttaacct cttttacgag attttcttga catattatca
     3001 gttagataaa ataattaaat aatataatca gcaagtatgt tttttactct tttaagtaaa
     3061 tttccacctg caccccaatc aatttttaaa attccaattg cactgcctgt tgaagtagct
     3121 ttaataactg tttttcctgt ggtattttca taaactacta ttaatcctgc atcactatta
     3181 tttcttgcat aatacttctc aaaagttgta attagtataa atttatcatc tcctaaatta
     3241 taagaatcac taaaaactaa atctgcatca agattttctt ttatcatttt ttgtgcctct
     3301 aagggtgtaa tttttacagt aaatatttct gttgccattt tttatctcct cataagaatt
     3361 tattttagac aaaacataat ctctagtatt ttctgatata tttatcaaac aatatcccta
     3421 ttcctcctaa cagagctaat atagaaccaa aatttctata ttgagaaata gattggtcta
     3481 gataaatatt atattggtca gtatccatga ttccaccatt ttgttgaata atatgctctg
     3541 cactaaattc acctaaacct atacaacaga ataatactac tactccaatt acaaaagcaa
     3601 atatacatat tgttaattta aaatccttat tttccatagt tatccctctc ataagctaaa
     3661 tttatttgat tatttttact agattttata ttactcttat tatacaatac aacatatatt
     3721 tacatctatt tccaaaattg taacctttat agttttatat atttaaattt tttaaacaaa
     3781 aaatacacat ctataaatta taaatgtgta ttctgtacca ttactaataa tatattatac
     3841 ttttttacaa atttctaatt atttataact attcaaaaat aaaattcata taaaataatt
     3901 ctattgtaca attgaaactt taataatgtc tccatttttt ctctccaagt gaactacttc
     3961 tccagttaaa tcatagtcaa atgccttaac taatatttca gtaacttttt taccatctac
     4021 tacatcatct tttccattaa ataaatccaa agcattattc tcaatcattc tctttgcaaa
     4081 atttacaggt aactttatat gtattttatc gcctgtatgt gataagactc ttattcttaa
     4141 aaacttttct aaattgtttc ttggtatttg ttttgatgcc atgctattaa atcactctcc
     4201 tttatttttt atttaataat aaattttatt cttaaaaata tctttatatg tatttattat
     4261 gaaagtaac
//
LOCUS       DAMJYC010000188         4141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJYC010000188 DAMJYC010000000
VERSION     DAMJYC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.195"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            41..439
                     /locus_tag="PC472_003895"
     CDS             41..439
                     /locus_tag="PC472_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2729164.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            427..1365
                     /locus_tag="PC472_003896"
     CDS             427..1365
                     /locus_tag="PC472_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2729165.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1749..1973)
                     /gene="secG"
                     /locus_tag="PC472_003897"
     CDS             complement(1749..1973)
                     /gene="secG"
                     /locus_tag="PC472_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2729166.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2566..3858)
                     /gene="eno"
                     /locus_tag="PC472_003898"
     CDS             complement(2566..3858)
                     /gene="eno"
                     /locus_tag="PC472_003898"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2729167.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1436 a          662 c          461 g         1582 t
ORIGIN      
        1 ataaatttaa ctaatttata taaaatctta aggagttgcc atgataaaaa aaatagaaca
       61 tgtggtcatt accaataata atatagaaaa ttatatcaat ttttataaaa aatttggttt
      121 ttcatctaaa gaaatgagca aaaattataa acttaatgct agaaatgtta agataaatct
      181 taacataaaa ggttataaat ttttacctta tgttaaaaat tcaaaagcaa gtagagataa
      241 cttatgtttt aaaataaaca caaatattga tgagccaaaa aaatacttag aacaaaatga
      301 gattcaaatt gagcgaggaa ttgtagaatg tacaggtgtc tttggtaata tgaaatctat
      361 ttatttgaaa aaaactaatg gaaatttaat tgaattatca aatcatagaa tcacggagag
      421 taaaccttgg aaaatttaat cctatcaata aatgttgttc ttcctttgtt tctcacaatg
      481 tcacttggat atgttctaaa aaaattaggt ctatttgata ctcatacttt aaataaaatg
      541 aataatgtct gcttcaagtc tttcttgcca ttattattat tttttaatat atacaataca
      601 gatttagatg gttctcttaa ttttaagctt atggctgtag ctgttataat tattttttca
      661 atcttcttaa tcttagtctt tattattcct aaaattgaaa aagataataa gacaaggggt
      721 gcattaatac aaggtgtatt tcgtagcaat ttcgttatat ttggtattcc tgtgacaatt
      781 tcactatttg gcgataagag tattggagtc acatctttat taattgcagt tattgtacct
      841 atgtttaatg tattatctgt tgtaattctt gaaattttta gaggtggcaa agtaaatata
      901 aaacatatga ttaaaagcgt cttaacaaat ccactcataa tcgcctcttt tataggattg
      961 acaatgctaa ttttaaatat taatatacct acatttttac acaaaactat tggtgatatt
     1021 tctaaaatag ctactccttt atcacttata attctaggtg gctcttttag tttttctgct
     1081 ataaaggggc atataaaccc cattatattg ggcgtatgtg gaaaacttat acttgtacct
     1141 gctatatttt taccaattag catttttctt ggatttagaa atgtagaatt agcttcactt
     1201 atgataatgt tatctgctcc aactgcagtt tcttcattta caatggcaca acagatggat
     1261 tctgacagtg aattagctgg acaaattgta gtatttactt ctggattttc tataattaca
     1321 gtatttttaa taatatttat tttaaagcag aataatctta tttaatgata tttgataaca
     1381 caaattggta ttatgagaaa catatataaa atcgatttaa ttgcgtaaaa acatattatt
     1441 atactatttt tactatataa tataactttg tgtatattat ctcattaaac atggtgaaag
     1501 tagtgttgct atatattttt tagtttgaaa aaaatatata aatacaaaca atatataaat
     1561 acaaataata aacgccaaca aatagtcaat ctataaaata aaaactggac taatcatata
     1621 aaatcattag tccagctttt attttacgta ctttttatgt tgatgtactt ttattaaaaa
     1681 ctaaatttaa tttttaaatg tctagcaaat gcactaatta aataatggcc agttattcaa
     1741 caatatcact acttcttaac tataagtaat gccaatgcat ttataaaaaa taatactgct
     1801 gttatctttg taactctagc aagaaatgct tgttttcctt ttggtttaaa gtaagcttga
     1861 cttccttctc caccaaattc tgaaggtaaa gcattcttag aatcttgagg cattatgcta
     1921 attactagta aaatccctag tactacttgc acacccatta aaatgttgct cattcttgcc
     1981 cctcctatct gtttattact taaatttcat tactctttag tgtatcacaa taatatagcc
     2041 atttcaagat tattatgcta taaataacat acaaaaaaat atattgttac tataatatat
     2101 tactatattc tcaatataaa aatgaataaa acttagaaga gaaaactatt taaattaata
     2161 cataaattaa cacttaaaat aatctactct cattgtatat attataaata gccaccaaaa
     2221 taaaacaatc attttaaaat attgatataa cttttaattt caacataatt taaaataaat
     2281 aatccacatt tagcaataat acttcaatga caattatgat taaatcaaaa tatgtcttca
     2341 cttttatttt ttaataaaaa cctcctatgc aattagataa aaatgagata gaaacattta
     2401 ttcatatttt ttctgaatct atgaataaaa ttaagtaata aaatataaaa aaggataagt
     2461 gaattgaaaa atcaattaac acttatctct tttatatctt ataagatgag cttaaatatc
     2521 ttatattctc tcttcattaa tcttttttag taaccatttt aataactatt tttttaagtt
     2581 atagaaagat tttaatccac aatatctagc ttgctctcca accatttctt ctattcttaa
     2641 taattggttg tacttagcaa ctctatcagt tcttgatgga gcaccagttt ttatttgacc
     2701 agcatttact gcaacagcta aatctgctat agtagaatct tcagtttctc ctgatctgtg
     2761 agatataaca gcagtatatc ctgctctctt agccatttct atagcatcta aagtttcagt
     2821 tattgtacct atttgattaa ctttaactaa tatagagtta gctactccat tttcaattcc
     2881 tttttctaat ctctctgtgt tagtaacaaa taagtcgtca ccaactaatt gtaatttatt
     2941 tcctaatttt tcagttaata atttccatcc atcccaatct tcttcgtcta aaccatcttc
     3001 tatagttatt attgggaagt tgtttgacca atcttcatat aaagcaacca tttcagcagc
     3061 agttaactct tttccttctc ctgctaaaac gtatttttta gtttctttgt tgtacatttc
     3121 tgtagcagca acgtcaagac ctaacataac gtcttcacct ggcttatatc cagcttttgt
     3181 tatagcttca actattaatt ctaaagcttc tctgtttgaa cctaagtttg gagcaaatcc
     3241 accttcgtca cctacaccac aagctaatcc tttttctcct aaaactttct ttaacgaatg
     3301 gaatacttct gctcccattc ttaaaccttc tttgaaacaa caagctccta ctggtaatat
     3361 cataaattct tgaacatcaa cgttgttatc agcatgctct ccaccgttta atatgttcat
     3421 cattggaaca ggtaattgtt tagcatttac tccacctagg tattggaata atggtaaacc
     3481 tatttcatca gcagctgctc tagctactgc cattgaaaca cctaatatag cattagctcc
     3541 taatttacct ttgtttggag ttccatctaa ttctatcatg attgcatcta tagctggttg
     3601 gtcaaatgca tccattcctt ctatttcagg tgctattatt tcatttacgt tagcaacagc
     3661 tgtttctaca ccttttccta agtatcttcc cttatctcca tctcttaatt caacagcttc
     3721 aaaagctcct gtagatgctc ctgatggaac tattgctctt cccattgctc catcttctaa
     3781 tactacttca acttctacag ttggatttcc tcttgagtct aatacttctc tagcatatac
     3841 taattcaata actgacattt gtatatctcc cttcgatttt ctattagtta aattttcatt
     3901 tactgtaaac atccgtctac tattttaatc tactttctat attatagtac ataaagttgc
     3961 ttttgaaaat taggtacaaa tttatttgat gggtaaattt gtaaaagttc ctatttttat
     4021 tttatttttt ataatatttt aatttatata atataaaaaa acttgtctaa aatccaaaac
     4081 caatcttaaa tacacaaata ttataactag atttaaaaca ttaaacataa aatgtacaac
     4141 t
//
LOCUS       DAMJYC010000189         4066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJYC010000189 DAMJYC010000000
VERSION     DAMJYC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.196"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..2286)
                     /gene="carB"
                     /locus_tag="PC472_003899"
     CDS             complement(<1..2286)
                     /gene="carB"
                     /locus_tag="PC472_003899"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF2729168.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAIC"
     gene            complement(2479..3528)
                     /gene="carA"
                     /locus_tag="PC472_003900"
     CDS             complement(2479..3528)
                     /gene="carA"
                     /locus_tag="PC472_003900"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2729169.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(3785..>4066)
                     /locus_tag="PC472_003901"
     CDS             complement(3785..>4066)
                     /locus_tag="PC472_003901"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729170.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         1169 a          803 c          411 g         1683 t
ORIGIN      
        1 acatatagca tctacttcta tttctctacc agttagatat ttatctataa gtactggatt
       61 tttatggtct ctatcaaatg catcatccaa atactgaatt agtttttctt cgttataagt
      121 tatttccata ccttgaccac caagtacata agatggtcta actaatactg gataacctaa
      181 ctccttagca atttcaacac cttcattagt tgtccataca ccttttcctt ttggtctatt
      241 tatatctagt ctctctagta aatcatcaaa tttttctcta tcttctgctg catctatatc
      301 tctaaagtct gttcccagta taggtatatt tttttcatgt aaaaacttag ccaattttat
      361 agctgtttgt ccaccaaact gtagtattac accttctggt ttttcttttt ctattatatt
      421 taaaacttct tcttctgtta atggttcaaa atacaactta tcagatgtat caaaatccgt
      481 acttacagtt tcagggttat tgtttacaat tatagtttct attcccattt ttcttaaaga
      541 ttttacacag tgaactgaac aatagtcaaa ttctattcct tgacctattc ttattggacc
      601 agaacccaat actacaactt ttctcttatc actaactact acctcatcat attgttcata
      661 agttgaatag taataaggtg atagagcttc aaattctcct ccacatgtat caaccatttt
      721 ataaactggt tgtatattat ataaacttct caattcatat aatttttctg ggcttatctt
      781 cattaagtca gatatacctt tatcagaaaa tccctttttc tttaattcat gtaaatattc
      841 tttatctaag tcttctattt ttagaacttt taacttttct tcttgttcta ctatccattt
      901 aaatttattt ataaaccact tatcaacacc agttatttct gatatcattt ctactttata
      961 gcctcttctt atcatttcag ctaagtcaaa tagtctctca tcatcaggta ctactactct
     1021 agcctttaat tcttctaatg ttctaccttc tgatggagca tgaaccaatg aatattttcc
     1081 tgtttcaagt gatcttatac ctttaagaat agcagcctca aagttacttc ctatactcat
     1141 gatttcacca gttgccatca tcttagttcc taattttcta ttggcatgtc taaatttatc
     1201 aaatggccac tttggtattt ttactaccac ataatctaat gttggctcaa aacaagcata
     1261 agttttttta gttactgcat tttctatctc atccaaagta taacctaaag ctatcttagc
     1321 tgctactttt gctattgggt atcctgtagc ttttgatgct agggctgatg acctactaac
     1381 tcttggattt atttctatta tcgcatattc taaactatgt gggtttaaag ctatttgaac
     1441 gttacatcca ccttgaacct ctattgcatt tattatgtct attgatgcct ttctaagtag
     1501 ttgatactct tcatcactta gagtttgact tggagcaaca actatactgt ctcccgtatg
     1561 aactccaact gggtctacat tttccatatt acatacagtt atacagtttc catttccatc
     1621 cctcattact tcgtattcta tttctttcca accttttata ctcttttcaa gaagaacttg
     1681 tccaacagga cttagctgta gtccatgtga aagtatttct cttaattctt cctcagtatc
     1741 agctatacct ccaccagttc cacctagcgt atatgctggt cttacaacta ctggatatcc
     1801 tatcttattg gcaaactcaa gacctgcttc taaatttgta actatatcac taactattac
     1861 tggttgattt atttctttca ttacttctct aaataaatct ctatcttcgc ccttttttat
     1921 agattctata gatgttccta taacttttac attatattta tctaatatac cagcatcatg
     1981 taattctaca gctaagttta atcctgtctg accacccata cctgcaagta aactatctgg
     2041 tttttctttt tctattattt tttctataaa ttctattgtt aatggttcta tatatatttt
     2101 atctgcaatt tctttatcag tcattatagt agctgggtta ctatttacta atacaacttc
     2161 aattccctct tcttttaatg cttggcaggc ctgtgttcct gaatagtcaa actctgcagc
     2221 ttgacctatt attattggcc ctgaccctaa tactaaagtt ttctttatac tatctaattt
     2281 aggcataact ctttctcctt atctaaacaa ttttcacagt tcttatcttc atcatctata
     2341 caatcaactt taattacttt attttatctt aaattcaaat ttactcatca atacatctat
     2401 ttaatacatc tatgttaatt aaaccaaact ttttgattta agttattctt gttttttagt
     2461 tagctattct aatgttattt attttactaa ttctaaaaat ttatcaaaaa tatagtcatt
     2521 atcttttggt cctgggcaag cttcaggatg gtattgaaca ctgtatacat ctagtttctc
     2581 atgtctcatt ccttcaacag tattatcatt taaatttata tgtgtcactt tcatttgttc
     2641 aggaacttct gaaacataat aaccatgatt ttgagaagtt ataaatactt ttccttcttc
     2701 taaatcttta actggatgat ttccacctct atgcccaaat ttaagtttag cagtttttcc
     2761 tccaagagct aaagctaaaa gttgatgtcc taagcatatt ccaactatag gttttttacc
     2821 aataagagct tttatatttt ctataacatc ttctaggtct tctgggtctc caggtccatt
     2881 tgacaagaat attaagtcag ggtttatact caatacatct tctggagatg ttgtagctgg
     2941 aaatattgtt atatcacatc cacgagctat aaatgatctt aatatatttc tttttactcc
     3001 aaaatccata acagccactt ttggaccatt tccctttata tgctctactt cttttcttgt
     3061 aacagttcta actgcttctg tatttgaaaa cttatccaat ttatgtttaa catcttctaa
     3121 tttactgtct tctaatgtaa ttataccttt catggttcca ttatttctaa gtatcttagt
     3181 caaagctctt gtatctattc cttcaagtcc gataacttta ttttgcttta aatattggtc
     3241 tatatcaatt tcacatctaa aattacttgg agaatcactt ttctccctta ctatgaatcc
     3301 ctttacttga actttttcag actctgctga tgataaattt ataccataat ttcctacaag
     3361 aggataagtc atagtaacta tttgaccata gtaagaaggg tctgttaaaa cttcaccata
     3421 tcctatcatc gaagtattaa atactacttc tcctacactt tcttctaaat atccaaatgc
     3481 ctttcctata aaaactgttc catcttctaa tattaacctt gctttcatac tacagtacct
     3541 cctataattc tctttagaca aactaaagaa cattaactaa atataagctt ttaaatatct
     3601 atatctattt ttcactttta cattctagtt ttatacttat ttacatttat tttttatctt
     3661 tttctattat actacaaatt tcttaaaaaa aagaagttat ctcaaaagta aaaactccta
     3721 gaaaaaataa cctctttttt ttatttaata attaaattct taaactatac aatcaactta
     3781 atttctattc agctatattg taaactttta actcatcttt atttaaatgt ttcttcttaa
     3841 cttctactaa agcttttagt gtatccaaag atgtcattat ttcagtagaa aattcaatag
     3901 ctgttcttct tatcttaaat ccatctcttg tagagtcatt tcctttagtt ggtgtattta
     3961 ttactatatc aacttgttta tttcttatag catctaatat gtttggtcta gattcttcta
     4021 ctctatttac tatatcagcc tctattccat tttctcttaa gctatt
//
LOCUS       DAMJYC010000190         4058 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJYC010000190 DAMJYC010000000
VERSION     DAMJYC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.197"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            93..1496
                     /locus_tag="PC472_003902"
     CDS             93..1496
                     /locus_tag="PC472_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2729171.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1587..1827
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1870..2568
                     /locus_tag="PC472_003903"
     CDS             1870..2568
                     /locus_tag="PC472_003903"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2729172.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELLNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2591..3592
                     /gene="trpS"
                     /locus_tag="PC472_003904"
     CDS             2591..3592
                     /gene="trpS"
                     /locus_tag="PC472_003904"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2729173.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1528 a          454 c          727 g         1349 t
ORIGIN      
        1 ttgaaaaata tcagtttaat aataatgaat ctattttatt aattttgaaa ttaaatttaa
       61 aataattaat agagagggga aattaaatta ggatggaaaa gaaaaaaata ggcctatgga
      121 gtttggtatt tatgaatgta tcagctttat atggtataag atggatagct aaatctacag
      181 catcaagctt tggtttagga ttaggtgcaa tacctatgtg gttattattc tcatttatat
      241 actttgtacc aggagcttta atttgtgctg aattagcttc tacatatcaa aaaggtgatg
      301 gaggtcttta cgattgggtt aaggaagcct ttggagaaaa gtatggattt ttagtttctt
      361 ggttaaactg gacagctaaa atattttggt attcttcatt tttaactttc ttagcagtaa
      421 atgtttctta tacaatagga aatccagccc ttgctgataa taagatgttt gtacttatat
      481 tatctttaat aatattctgg gcattatctc ttatagcaac taaaggaata agttttacaa
      541 aaatatttac taatacaggt gcattaggtt caacaatacc aagtgctata cttataatat
      601 tttcatttgt agcagtattt gtgttgaaaa aacatgatat agcttctaca tacacaatag
      661 cttcaatgac tccaaaactt aatatggatg catttgttgc tatatcagct ataatgtttg
      721 gtttagcagg agcagagact gctgctaact tcataacaga aatagataag cctgaaaaga
      781 acttccctaa ggcaatatta atatcagctg gaatagtagc ttcattatat gtattaggtt
      841 caattgcaat aacaatgata ataccaccag accaaattac tgcttctaaa ggtgtattag
      901 atgcactttc tgctgtttgt gcgtctttag gaattggttc ttggttggtt caattaatag
      961 cttttggtat agctttttca gtattaggag caatagttct ttacatagca tcaccaataa
     1021 aaatgttatt tggtagtgtt aaaaaaggaa tatttccaga tagtttagta gaggtaaatg
     1081 aacacaagat accttctaaa gcagttatac ttcaagcaat aatagttact ataatactat
     1141 tggtgactac attaatgcct tctgttgatg ctatatacaa tgttcttgta actatgacag
     1201 cattaactgc attattccca tatgtattgt tatatgcttc ttatattaaa ctaagaaaag
     1261 agagaccaga tgaaattaga ccttatacta tggcaaaatc aacaagtact tgcctaggtt
     1321 tagcaaaaat ggttttagtt gtaactgtag tgggtatatt attatctgca gcaccagtta
     1381 tgcctacatt tgctgaaaat gttatatatg agatagagat gattggtgga ggtcttctag
     1441 ttatattatc tggtctatgg ttatggaata gatatgaaaa gaaaacagct aaataaaatg
     1501 tattttaaaa atttcttttg gatattataa tatatagttg acaatttata gtgtaaggaa
     1561 tattataaaa taaagaaatt aataaattaa atcctgagat aagaaatagt aaataaatat
     1621 atttttgcac agagaattaa ggatgctgag aacttaataa aaagtattta ttgaatgggt
     1681 ctttgaggag taaaatgaaa tgtaatttaa gattacagag tatttgatat cgtgtatgca
     1741 cgttatagca tatattaaga tatatatatt caattttgag tgtatataaa tttaggtggt
     1801 accacggaaa atatatccgt cctattgatt taggacggat tttttaatat agaaaattta
     1861 ggaggaaaaa tgaacgtaat aggaattata ggtgcaatgg atgaagaagt tagtatttta
     1921 gttgatttaa tggatattag agagactata aaaaaagcta gcttagaatt ttataaggga
     1981 attttggaag gtaagaatgt agtgttagta aagtgtggca taggtaaggt taactctgct
     2041 ctttgtgccc agatactaat aagcgaattt aaggttgatg ctattgttaa tacaggtgtt
     2101 gcaggagctt taaacgaaaa acttgatgtt aatgatatag taatatcaac tgatgctata
     2161 cagtatgatg tagacactac agcatttgga gaccctaagg gagttatacc aagaatgaag
     2221 acttctgtat ttaaagcaga tgaaagactt atagatgctg cttacaaatc ttctgtggaa
     2281 gaagtaaaga ctcataaagt attaaaagga agagtagtta caggagataa atttataaat
     2341 tctaaagaat taaaagaaga attattaaat gattttggtg gatattgtgg agaaatggaa
     2401 ggtggagcta tagctcatgt atgctattta aataatactc catttgtaat aataagagca
     2461 atgtctgata aggcagatgg aagtgctgat gttacttatg atgtatttgt acatgatgct
     2521 gccaataact ctaaagacat agttttaaat atgttaaaat caatataaat atataaggag
     2581 gtacaaaaaa atgagtgaaa aaaaagttat attaagtggt gcacagccat caggcaaaat
     2641 gactttagga aattatttag gagctataaa aaactggact gaacttcaag acaattatga
     2701 ttgttattat agtatagttg atttgcatgc aataacagta cctcaagacc ctaaagtttt
     2761 aagagccaat actatagaat tacttgctca atatatagct tgtggattag acccagaaaa
     2821 aaatactata tttatacaat cacatgtcaa agagcatgtt gaacttatgt gggtattgaa
     2881 tactatgact tatatgggtg aattaagtag aatgactcaa ttcaaagata aatcacaaaa
     2941 gagtgaagca aatctaaatg caggtttatt cacatatcca gttcttatgg ctgctgacat
     3001 attgttgtat caaactgacc tagttccagt tggagatgac caaaaacaac atttagaatt
     3061 agctcgtgat ttagcaaata gatttaataa tagattttca cctacttttg tagttccaga
     3121 aggatactac ccaaaaggtg gagcaagagt gatgagtttg caagagccaa ctaaaaaaat
     3181 gagtaaatcc gattcaaatg aaaatgcatt tatactatta gcagatgatt cagattctat
     3241 aaagagaaag ataaaaagaa gtgttactga ttctttaggt gttgtaaaat acaatgatga
     3301 acaaccaggt attaaaaatc taattgatat atattctaat ttaagtaaaa agagtgtaga
     3361 agagattgta aacatgtatg aaggaaaggg ttatggcata tttaaagaag atgtggctga
     3421 agtagtatca gaagctttaa gacctataag agaaaaatat gttgaccttc ttaataataa
     3481 agattattta gaaaaaatat atagtatggg agcagaaaaa gccgaaaaac aagctagaaa
     3541 aactcttaga aaagtttata aaaaagttgg atttatagag agaagatact aattattagc
     3601 tatctttact tgataaataa attttaaagt tattaaaaag gtatatgata tgaaagagag
     3661 agtctcaaag tgaacttttg ttcatgaggc actctttttt gtatgaaata aaatatattt
     3721 taatcatttc aacaatgaaa aagaggatta tctctatttt gagacagcat cttttgataa
     3781 taaaaaaaga tgagcttttg ctcatctcaa aggggttaga ttattcttac tttggggaaa
     3841 gtagaataaa ggtaaaagtt aactgatgtc aactttgtct tttttgcata tcactactga
     3901 gcataaaaaa atcagaaaac catagccttc tgaaacaact tctaacatac attgtatttt
     3961 atacccaata tgtaatttat gagttaattt taactcttat aatgaaaatt gtcaataata
     4021 tatcaaaaaa atatttttta aatttatata attaaatg
//
LOCUS       DAMJYC010000191         4012 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJYC010000191 DAMJYC010000000
VERSION     DAMJYC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.198"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(332..853)
                     /locus_tag="PC472_003905"
     CDS             complement(332..853)
                     /locus_tag="PC472_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2729174.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1019..1585
                     /locus_tag="PC472_003906"
     CDS             1019..1585
                     /locus_tag="PC472_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2729175.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(1802..3854)
                     /locus_tag="PC472_003907"
                     /pseudo
     CDS             complement(1802..3854)
                     /locus_tag="PC472_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
BASE COUNT         1295 a          624 c          372 g         1721 t
ORIGIN      
        1 aaataaaact aaaaaagatg ttgtctcaaa atatagataa atttattttt cattgctaaa
       61 atgattaaaa tatatttgat ttaatatact tgatttaata taaaaaaaag agtgtctcat
      121 aaactaaaaa gttcactttt agacactcct ttatatatat tatctacttc ttaattcatt
      181 tattacctct ctacattttc tttcaactag tggatacatc tttataagta ctaattactc
      241 cacttaaaaa tgattcaggt actgtccatc agtcatattt ttctgccaaa tataatggtg
      301 tttcataatg agacaataaa tatttatatc tctacttatc ttctctaagc tcgtcaaatc
      361 caactatagg tatatttaaa ttatattctt taagtagctt ttctagctct tctataaaaa
      421 tacctttccc atccaccatt tgtatatcct ttattttatc catcaattca ttgcaaccac
      481 ttaactcacc aaaccaacgt ttagaactac atgtcttatt ataaccacaa cttggactgc
      541 catctattgc aatgatactt tttatatcat aatcattttt taaatattca tcaaactgcc
      601 ttacatgagg tattaacatt tgcctacatt gttctctaaa aaacaaattg tcaaactgtt
      661 cttttacatg tccccaccta tttattccat acataatcat ttctgggcat ggtagttgaa
      721 ttattccata ccctttattt attaaattta tcgctatatt ttgtgctccc ttaaattctg
      781 acaatccctc cactttagaa ttacaattta atatacaatg acatactact acaatctctc
      841 tatttctttt cataataatc tccttagtta ttcttaattt taatgatact acatattttg
      901 ataattatat tatttatttg ttctatattt ccaatattct atatagaaat atacaattag
      961 taatctttta taatctatac tatgataaat gtatatattt ttttaggagg tattataatt
     1021 ggatactaaa aaagtcacat tttcttcaat gtgcatagtt attaatattg tcttaggtat
     1081 ggttatatct atgataaata taccattatt atttttagat acagttggta ctgtacttgg
     1141 ggctgtcgta ttaggtcctt tttggggtgc tcttattggt gggtgtacaa acttagtttt
     1201 aggtgttata tcaggtccta caaatatacc ttttgccctt gtaaatattg ttcttggtct
     1261 tattgttgga tatatttcta agaaaaaagg ttttggttat aaagaagcta ttattacagg
     1321 agtaatttta tctattgttt gtcctttaat tggtactcct atttcagttt tattgtttgg
     1381 tggtttaagt ggaagtggtg ctgatttatt ggttggtttt ttagttcagt ctggtcagaa
     1441 gatatttact gctgcgttct ttccaaggat tcttagtaat atagtagaca aacctttgtc
     1501 ttgtattatg gtggtatatt ttataagtaa aatgcctaaa ggttttataa aacaatatag
     1561 taaaaatact tctagttcat attaataaat catttattat gtaaggttta ttactcttta
     1621 aaatgctata tactcatttt tatataacat ttttattaat aatttaatta gatagtattt
     1681 gtattacttt aattaaacgt aacctcttga tataaatttg agctataaat ctatatcaag
     1741 aggtgctatt ttatttttaa tataattaat attctattac gcaaaaaatg catatatttc
     1801 attattttat ggaaaatatt acttcattta actctatatc aaactcatta agtaaaaatc
     1861 taattatatt taatttagcc tctgtattta aatttgtttc taaatatata ttttctgata
     1921 ttttaatacc ttttcttaat ttatatgcat ctctagactt aatattatct ataaaatctc
     1981 ttttataagt tttgtctaca atactcagaa tccaaatcat ataaagtaag agcaacttgt
     2041 tcataaaatt ctgtccaatt tttagcctta tatgactctc ctaatatact aaatgatact
     2101 atactttcat ttgtaaaatt aacatcatca tcctctaacg taaatatatt ttcactgtta
     2161 cttggaggtt catatgtaac acttggatat ttccaaattt ctacagctct atctagtaat
     2221 atcttagttc tattttttat ttcttcttca ttccacttat ctatagataa aatatattta
     2281 tttagataaa gcctactatc tttaaatcca tcttccattt ctttcttttc ttcaaatgtt
     2341 ttattactta gttttgaatt atatcctgtc aaagttaaat ttccaattgt atgtaaatat
     2401 ttatcatgta tctccttata gttatctcct agtgattcct tccatttatt tgttaatgtc
     2461 tgtggcatta tatgctctat atttagcata ttattattga ttaaattttc taaatcaaca
     2521 atttctttat tattataatt ctctaacctc tcaagtatat ataacttatt ctttgactta
     2581 gtattatata tatctttagt ttcaaaagca tttgaaaatt cactgtcagt aggaaatctt
     2641 tgagatgatt tcttatttat aaaaatatat cttaatatat tagaatagtt ttctttataa
     2701 tcactaaatt ttttaatttc ccttcctaag ttcataaata ctttattcag tgcattcgga
     2761 ggtacatcac atactattct tcttaatata aaacattcta ctaaattaag ggattctaca
     2821 agtacttttt tatttattat accttcatta taatcatcaa ataattctaa tataaacgga
     2881 tatgatacaa atatttcaag tttgtttatc cttttcaaaa ctttgtttac atcattatca
     2941 tcagtacatg aataagatat ctctctataa tagttagaaa atttaagtaa atcttttaat
     3001 aaaatttcta tatctatacc attatctgat acataccttt taaaactaaa atatacttta
     3061 tttttattcg gtatacttct ctcctttata gtcaaatagt ctcttataaa actacttacg
     3121 ttgctacttg tatttttttc aattttatac caataattat tataaaattc ctcttgtaac
     3181 ttagagcttc tacccattaa cacaaagttt cttactctat cagcttcact taaatctaag
     3241 ccagttgaat ttagactttc aaagattaat tgagggtcat cttcaccatt ttttaactct
     3301 atttctacta tagttaattt ctgtatagca tcaaacaaat catctattga tatattatct
     3361 tcttttattc tattataaaa atataaataa tttgaaacta tattagaatt tctatctgta
     3421 ttcatttctt cttcaaatag ttcatagtac gcttttctat cttctgctat tggttttaac
     3481 tttatttttc catctttatc tcttgagtgc ttgtttataa gataacactc cttaatctgt
     3541 tcttctgttg taataattcc ttttgattcc ccaaactctt ttattatttg acacatggca
     3601 atcaataata ttgacacagt agtcactctt tgttgtccat ctattattaa atattctttt
     3661 cccatactat atatagatac aactgaccct aagaaatgag ttctaaatcc agaatctatt
     3721 atatcctcta aatcatcaaa caatctttta cattgttctt tcttccagtt ataatttctt
     3781 tgatatacag gcactacaaa acttttgttt gacccctcta aataactaag aaattcaatt
     3841 tctcctgctt tcatattaac tccccctata agttaaataa attccattca taactccatg
     3901 tatctatata ttatatcaaa attttgactc ataaatgtat atgaaagcct tattatatac
     3961 ctttttatac ctatttttat tttattaaag taaatacatg attattctat aa
//
LOCUS       DAMJYC010000192         4007 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJYC010000192 DAMJYC010000000
VERSION     DAMJYC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.199"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            592..1347
                     /locus_tag="PC472_003908"
     CDS             592..1347
                     /locus_tag="PC472_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2729176.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(1490..1918)
                     /locus_tag="PC472_003909"
     CDS             complement(1490..1918)
                     /locus_tag="PC472_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2729177.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(2339..2539)
                     /locus_tag="PC472_003910"
     CDS             complement(2339..2539)
                     /locus_tag="PC472_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2729178.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(2722..3891)
                     /locus_tag="PC472_003911"
     CDS             complement(2722..3891)
                     /locus_tag="PC472_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2729179.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         1349 a          621 c          458 g         1579 t
ORIGIN      
        1 atttaaaatt ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg atgaatgtat
       61 aaagaaaagt tgtttgataa ttattttaag ttcttagcac tattattttg gccaattatg
      121 tggtataagt ggatagtaat ttcaaatggt actcttgaaa atatgttatt taccatatat
      181 gcaatcgtcg caattgtttt catcatttta tattcggtgt ttatgataaa atataaagac
      241 attacacaaa tagatttctt ttacaagata agtactctac ttgcttttat atttacactt
      301 tttagctttt taatataccc aaagagttta tttttcttgt atttaaagat tatttttact
      361 ggaatatacc tttattattc aatagtaaaa actcttaaat ttaaagatga cgaaggtgta
      421 gtgggtatta tgagctcact tttattaatt gtaataactt tgttctacta aatttaattc
      481 tatttgactt aatatggctt taaatattag acatctacat tattttctga tattatttaa
      541 gatggtagtc ttatattaat aatgaaatac actatgaagg agttaactat tatggaaaaa
      601 aaagttttag caacagttgg agaaaaagag ataactaata ttgatattga aaatgcatta
      661 aaatcattag acccttatca agctatgcaa tttaaaactg aagaaggaaa aaaacactta
      721 ttaaatgatt tagtgaatca agaattattc ttcttagatg ctaaagaaga aaaattagat
      781 gaagaagaaa tatttaaatt agaaatgaaa aagatagaag aaaatgtatt aaagcaattt
      841 gctataaata aagttttatc tagtgttaat gtaacagaag atgaaaaagt aaaattcttt
      901 gaagctaata agagctcttt ctctaaacca gaaagtgcaa ctgctaaaca tatattagta
      961 gatagtgatg aaaaagctaa agaaatactt gctcaaataa aatcagaaga aatatctttt
     1021 gaagatgcag ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg tgatttagga
     1081 acattcggta gaggacaaat ggttcctgaa tttgaagaag ctgtgttctc tatggctaaa
     1141 ggtgaagtaa gtgaaccagt taaaactcaa tttggatacc atataataaa attagaagac
     1201 cttcaagaaa gtacagagtc tacatttgat gaagttaagg ctgaagttga aaaaagttta
     1261 ttatatcaaa aacaaaatga agtttatgga aataagataa atgctcttaa tgctaagtac
     1321 ggaaaccttg taaaatacaa cgattaatca taaggtcata tataaaaaag ctgtcttttc
     1381 acaatattta ttagaaaaga cagcttttta tattaaagtt taaatattca gtttcatatt
     1441 atcattcttt tattgtaaag aactcagctt aaaagtttaa ataagatttt taattatcat
     1501 tagctaaatt tttatatcca cgtattatct cataacagta acctattaca tctcttcttt
     1561 ttattatccc aataaatgtg tcttggtcat ctataacagg aatgaaattt tgatttaaag
     1621 attttataac taaatcttca atatctgcgt tgatggaaac aggagaatta tccatttttc
     1681 tatttatatc tgtcactgga acatcttcca atccctttaa gtccagattt aaatcatttt
     1741 ttaatgtcca taaaaaatca ccttctgtta tagttccaac atatttacca tccttactta
     1801 ttattggaat tgcagagtat ttgtgatatt ccattttttc tagggcttgc cttatagtgt
     1861 agtcttcata tatatatgct acttcacttt ttggtgttag aaagaataat atgttcatta
     1921 cactacccac cgttactgaa aacttataaa tattactcaa tatttataat gttacttttt
     1981 tgcagtaact ttctcctttc ttattttcta agtattctta atactatacg ctgtaacaag
     2041 cgtttttcct ttgctcattt taatcttatt atgctttatt aatattttta tatggtctat
     2101 cagtatcact tattctcttt ttagaaaatt tactaaaaat taattgactt aaacttagcc
     2161 ataataattt tactctacac tattaatttt aacatattta tctcattatt aatgatttta
     2221 ttttaataat tttaatttat tttgctatta attttattta caaaaactga aaaaaccgct
     2281 aaatgattag cggcttatct ctcatattac tagaacacta atatatatga atttttaatt
     2341 ataatctaac tacgttagca gcttgaggac ctttagctcc atctactact tcgaactcaa
     2401 cgctttgtcc ttcttccaat gttttgtatc catctccagt tatagctgag aaatgtacga
     2461 aaacatctcc ttcaccttct acagatatga atccaaatcc tttttcgttg ttaaaccatt
     2521 taactacacc agttttcatt aataaatcct cctaaaaaaa cacaaatatt tagctactct
     2581 tttctgtaac cttattacta ctctatcata tatagctatg tttgtcaatt attattattt
     2641 ttatacatta ataatcaatt tttttacttt ataattttaa atgattatta tcttttacac
     2701 tttatttttc tttataattt tttaataatt tatttttatt atatggaatc ttatggactt
     2761 atcactccaa cctaaatccc atggcaccaa cattctatca gtattatgac aagttaccaa
     2821 aggataccct cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc tgcctttctt
     2881 ctcataacca acaaaatccc ctggacgtaa attataagct tctttgtata cttcttcata
     2941 ggaaccttta gcaattaatg aacctcttcc actatacagt gcataatttt taaaagcttg
     3001 tgcatttacc catgcttttg tacctgctct ctcgctataa ttccaaattg agttcttttt
     3061 gaaccttcca ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc caccttctgg
     3121 attataatcc ctatagtttg gattaaattt cataccatgt tcttcatcag ctgcggctcc
     3181 acagtattta tgtgcataat ctatagcctt tttttgttca tctgtaagct gtaactctac
     3241 accatcttgt tgcccaatat agtttcttat atcctcagat tttatatttt ctaaatttaa
     3301 agagtctgca aaagggtctg tgtaccactc tttagttatg acatattgat tatctataat
     3361 ttttacattt agataatgat atgtacctat tctaaaggca ttttctttgt ccttttgatt
     3421 ttcatatgaa tacttatatt cagtcgatac tgaacagatt ataccataga ggtctttttc
     3481 tttttctttt atttttttaa tcttaaccct tgtctttatg tctttaaatt ttacaccttg
     3541 cttactagac cagttttcta aatacttcat cttttttact tcattttcat aagcccatag
     3601 accaaatttt ttatccgtct cataaagacc ttttaaaata ttttcatttt tctctaatat
     3661 tgccttattt ctgtagtcaa ataagttttc aagcaatagt ttatattggt cttttacatc
     3721 atcaccttca acatttgcag attccatgac tttttcttcc atatttttca tacttaaatc
     3781 attgattaaa aatacacctg ctgtagtagc taaagacaaa aatacaaatg ccattatacc
     3841 tttgatgtac ttattcttat ttttcagtat tttcaaagct acacctccca aatagtacac
     3901 cttacaaatc aaattacaaa gaatattctg tcacatatag taattttcat atttaataat
     3961 tctctatttt attatgtcca atattaaaaa ttaattgtat caataca
//
LOCUS       DAMJYC010000193         3902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJYC010000193 DAMJYC010000000
VERSION     DAMJYC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.200"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            145..1650
                     /locus_tag="PC472_003912"
     CDS             145..1650
                     /locus_tag="PC472_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2729180.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1764..2462
                     /locus_tag="PC472_003913"
     CDS             1764..2462
                     /locus_tag="PC472_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2729181.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2747..3835
                     /locus_tag="PC472_003914"
     CDS             2747..3835
                     /locus_tag="PC472_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729182.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1530 a          391 c          636 g         1345 t
ORIGIN      
        1 ataaaaaaaa ataattaata taaaaatgtg tacaaactaa tgaatattga tatagtaagc
       61 tatcatatga aagagtatgt acacattttt tttactaatt tttcacaata tgaacataaa
      121 taatttacag gaaaggaatc ttttatgaaa attcaaaata ctgagtgggg atatattgaa
      181 tggaaacata cttatgatga aaataatcca aaacaagcta tgaatatata cattgcagta
      241 actatgcctg gtaagaaaca ttttaatcat gttcattatg gtcaagagca gatgatatat
      301 attttagagg gagagggttt gtatattata aatggagttt ggaagccatt ttatcaaggt
      361 atgatttttt atatagaatc tggtagcaca catgaaacta taaatacagg cgatagagaa
      421 ataaaagaac ttattgtttc aaataatgta gatgatgtag gagagagtga agttatagat
      481 attaatccta ataattattt aaagaagacc ttgataaatt atagtgaaag tacattaaat
      541 ttgtatgcag cagtagaatc aattcgtggt caatttatag acccgtttaa aattccatta
      601 attatatatg atgattcatg gaatatagtt ttaaaaaatc cttattttcc acttttttgt
      661 tttgaaaaat gtaatcctat gaaatttcct caaaattgtg attgtatgaa tcaaaaaagt
      721 agcaatcaat ttgtatgtga atatgggata actatatata atataccaat actgtacaaa
      781 agcaatagca taggtgttat aagaggaggg tatgtactat tatctgattt gaatttagat
      841 acagagcata ataatttata tgatattcca gagggagctg ctagaagtat aaaacgcctc
      901 ttgaaacaaa tatctaaaaa tataataaat ttttgctctt ttaatgatat aagaaaggat
      961 ttacaagaaa aagagaagac tatagcaaga acatatcatt atggagagca acttgaaatg
     1021 aatttaaaag tagctcaaga tatggttact aatttaagaa taaatcatca ctttttattt
     1081 aatacattaa attcaatggc aagcatagca ctagatgatg gtagttatga tttgtatagt
     1141 gcaataatag atttatcaag aatgtttaga tatacaatga ggtctgattt aagatttgta
     1201 gaattagaat ctgaaatatt atacattaaa aattatttga atttacagaa attacgttat
     1261 ggagatgcac ttaaagtaaa atatcttatt ccagagaagt tatataacct ttctgtgcct
     1321 tttaacttta ttcaacctat tgtagagaat gcatttacac atggatttag agatattgac
     1381 acagaaaagc gaattgaaat aattgcaaga ttagattctc agtatgccat tatagagatt
     1441 cataataatg gaactatctt agatggaaat aatatagaca aaataaaggc aggtattcgt
     1501 agtaataatg gtcatggttt gtctttaata tatacaaaat tcacatctgc ttatggaaat
     1561 aattttgata tggatataaa gtcgagtgat aatgaaggaa cttacataat gattaaaatt
     1621 cctattgaaa attataagga gtatgtatag aacttgtgaa aaattaataa gttttataaa
     1681 tgttaagaaa tatattagat gtttttatgt tgttttatat atcttacttt tttatattat
     1741 taagctaaaa gggaggtatt ctcatgagaa gagcactaat aatagatgat gaagaagctg
     1801 ttctaaaaat aataacacat tttatagaaa tgaaagatat gccaataaaa attgttggta
     1861 aagcaacatc tggtgatgaa gcagtagata aaatcattgg tttagagcca gacatagttt
     1921 ttatagatat tcaaatgcca atatacaacg ggttagaagt aatagaaaaa acatctcatt
     1981 tatctaaaaa aataaacttt gtagtaataa ctgcatttaa ctattttgaa tatgctcaaa
     2041 aagctttgag attaaatgta aaagatatac tccttaaacc attggatatg aaagaattta
     2101 ctaagtctgt agaaaaaatt attggatatc aatataccaa taatgattta ttaaatgaga
     2161 tactggaata tataaatcta aactatagca atgacttaaa gttaaatgat tgtgctagat
     2221 tatttttaac caatccatct aatatatcta ggatttttaa aagtaattta aatacaactt
     2281 ttatatcata tctaaatcat ataagaatag aaaaatctat tgagttactt gaaaatacta
     2341 caaaaagtat aaatgagatt gcagaaatag ttggatataa tagcctaaat aacttttata
     2401 aaaactttaa gatagaaaaa ggaatgactc caaaagttta taaactgaac actaaaaatt
     2461 gaaattgata aataaaaagt caatatagat aattgtttga gtgtgtctga ataagtatac
     2521 tttaattatg ataaaaatta aagcgcttta aacatattta ataatattaa accttataaa
     2581 agctgtacct atttatataa aaaaatatat ataaataaaa agaaaaagat atacaaaata
     2641 aaaaaagtga attaaatact gtttcaaaaa gacagaattt tcatttaatt tactgagagt
     2701 ttatatttct taaaggaagg taactttata atggaggtaa tttaatatgt cagacaaagt
     2761 gaatatgaaa aatattatct gtattgcagg cgcttatcta gcaacagcaa ttggttctgg
     2821 gtttgctact ggtcaagaaa ttctacaatt ttttgcctca caagggtata tgggtttaat
     2881 tggagcatta atatcaatgg tattgttttc atttcttggt gctgaagtta taactagagg
     2941 tagagaatta aaacttaaag aaccaacaag aatttacact ttttattgtg ggaagatttt
     3001 aggaaaattt tacgaatggt ttagtccaat atttttattt gggattctta tagtaatgat
     3061 ttcaggtgca ggctcaactt taactgaata ctatggtata aatccttact taggaagagt
     3121 attgatggct gttgctactt taattacagt gtctatgggt ttagaaaaat tagtaagcat
     3181 acttggacgt attggaccag tgatagcagt atttacaatt gctgttggat taataagctt
     3241 gtttaagcat tttgatggtt tatctatggt tggagaagct atgaaaaata ttgaagttac
     3301 aaaagcatca gaaagtccaa tcgtttctgg tgtaatatat aatacattaa atattattgt
     3361 aatgattgtt tttttaacag gaatgggagc ttctattgaa agcaaaaagg atgctttttg
     3421 ggggggttta ttaggtggaa tagtgtttat gactgctggt atagttatgt atttggctat
     3481 gatttcagat ataggaaatt tgtatatgaa agaaatacca tcactttatt tagcagataa
     3541 tatatctccg attgtaggtg tatgtttttc agttgcatta atattaggta tctatacaac
     3601 agctgttcca cttttatggt ctgtaaccaa tagaattgtt gaagatgaac atcctaaatt
     3661 taaattggta acaacagtaa tagctatttt ggcttgtata ggaggttttt taccgtttgg
     3721 taaattagta aacattttat atccatatac aggctatatg gggcttttaa ttttagtctg
     3781 tatggtgtat aaagcaatta catgtagaaa aaaatcaaca caaagtcagg aatagagcca
     3841 cttattaata gtttttattt aaagaaattt tttatataat ccgaatttaa aaagtcaaaa
     3901 ta
//
LOCUS       DAMJYC010000194         3901 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJYC010000194 DAMJYC010000000
VERSION     DAMJYC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.201"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..260)
                     /locus_tag="PC472_003915"
     CDS             complement(<1..260)
                     /locus_tag="PC472_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2729183.1"
                     /translation="MKVVIVPGHTLTGKGTGAVGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCEIANRQNVDLAVQIHFNANKTTLNP"
     gene            complement(260..526)
                     /locus_tag="PC472_003916"
     CDS             complement(260..526)
                     /locus_tag="PC472_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729184.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKVMLQKEGDK"
     gene            complement(573..743)
                     /locus_tag="PC472_003917"
     CDS             complement(573..743)
                     /locus_tag="PC472_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729185.1"
                     /translation="MYNILKRMIEQKNYETKEELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTPIEPII"
     gene            complement(769..1086)
                     /locus_tag="PC472_003918"
     CDS             complement(769..1086)
                     /locus_tag="PC472_003918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729186.1"
                     /translation="MNKIKFRIIREHNFSKEEIDNIGIDNFFIADNGQLWTPLISKGE
                     IIKTGEQSYNEWLELQINPVKPEQSLDRGTILEENQKEQDNLLIDNAYRIAILELNTN
                     NVL"
     gene            complement(1089..3419)
                     /locus_tag="PC472_003919"
     CDS             complement(1089..3419)
                     /locus_tag="PC472_003919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729187.1"
                     /translation="MLNEDVVKNINKLNNEQDNINLELSKKANKEDLEKLIQGGPNVS
                     VSKDISINNWILEENVYTAIVEHNLVTRKVIVSLIDKSTNNNVFCSYQILDDNSIKVF
                     NELNNELECIVVNGNSSINMISATIDDNRSTETTTYSSVKIEALLKVLENKIDDIENL
                     KEIDNIKCSTDTGEYIIENSKKGYLTNFNIEGKTLIDLWGKTSSDFSLWKATFVDGKI
                     NILTENDIRYSNFFTINYTSYKPDTIYTIIVDVDKNTLPSTSGIYIHSLGEENSVFIP
                     NLTNIAIPGGVIGKFKYTFTTISDLSDCNAVLRSVLDNDTLTSGYEVSLKITILEGDY
                     TDINIDYSNELLSVGQRDKIEFLSYQYSGINIFNKNADFKDNYILQYLNGEELISEAS
                     NHKYTLDYIEIEPDTEYTFYNCSRNICWYDINKSFIPAPLNERIIGDKDIFYVARSPK
                     NAKYLRVTIIKDLHDNGNKTIITKGNKYDKKTIPHTLRGLANGIRDEIVYKNGSYKLI
                     KRCEEIVFNGNENWIEVHGMYEFRLNLDISVLRLDMSKFITNLYKQSHVDYIFNVQTE
                     SALSISTENGIGVIDIVDTSISPFSIANFKEKLKKNPLKIIYELEIPREVELGLLNLE
                     QYDNQTGFICSSGITIPNVSFESTRNLGSHIEVIRENLKDITTLTESNVDFKFINGWD
                     KVWQSATITKVGKYLFVNMIVTKSTSSTVLEEAIIKLPLGISRQNYSCPVMNSSCEQV
                     DTVGIACVNNTILMLTPGHDITDKGILISVIVNLEV"
     gene            complement(3454..>3901)
                     /locus_tag="PC472_003920"
     CDS             complement(3454..>3901)
                     /locus_tag="PC472_003920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729188.1"
                     /translation="SISKSPLALNKILKTEIGRTSLANSSYLQTNYSNISTSTQNTTY
                     FAKGSNAVIDSGSRNSNDTGGMVSSTTPNQCIFICLTIGSWSNGTNTTGTISHLQTGS
                     QVATRTTTTGPIHQGTGATAQYICVGGCKFTESSDSGAWGCMAYAK"
BASE COUNT         1175 a          647 c          415 g         1664 t
ORIGIN      
        1 ggatttagag ttgttttatt tgcgttgaaa tggatttgta ctgctaagtc tacattttgc
       61 ctattggcaa tttcacattg ttctgctaaa tagttattag atttatctac ttttccagta
      121 tacacaatag ctccaccttg tttcaaccat ttaacaatta aatcagttaa aattctattt
      181 tcttttcctt catctatata accaactgcc cctgttcctt ttcctgttag tgtatgtcct
      241 ggtactatta ctactttcat catttatcac cttctttctg tagcatcacc tttatatctg
      301 ttacatcttc ctttatttcc tccacatcag ttttcattgc tcccatttca acaagtatat
      361 ttttattgat ttcttgttgt tgtgttgata gttcaataaa gttttctact gtctttttat
      421 acatgtctct atcttctttt ttctcttgca tagtattttt gaataataaa gcacataaga
      481 ttcctattgc tcctaaacta cttaattctg ttaataattg ttccatgatg acctcctact
      541 aaaattgata taaaaaaaga accttttata ctttatataa taggttctat tggtgtatct
      601 tctttgttta ataaatttgt taactctgta tactcacttt cttttattct gttcatagcg
      661 taaaatacat ctaacttagt ctgcaactct tctttagttt cataattttt ttgttcaatc
      721 attcttttta aaatattata catgttgttc cctccgtttt ttatagtttt ataacacgtt
      781 atttgtgtta agttctaata tagcaattct ataagcatta tctattaata gattgtcttg
      841 ctccttttga ttttcttcta aaattgtacc tctatcaagt gattgttctg gtttgactgg
      901 attgatttgt aattctaacc actcattata agactgttca cctgttttaa taatttctcc
      961 tttgcttatt aaaggtgtcc acaattgtcc attatctgca ataaaaaaat tatcaattcc
     1021 tatattatca atttcttctt ttgaaaaatt atgttctctt attattctaa actttatttt
     1081 attcattttt aaacctccaa atttacaata acactaatca aaattccctt gtctgtaata
     1141 tcatgacctg gagttaacat cagtatagta ttatttacac aagctatacc tacagtatca
     1201 acttgttcac aagaagaatt cataactgga caagaatagt tttgtcttga tattcctaaa
     1261 ggtaatttaa ttattgcttc ttctaaaaca gtagaagatg tactctttgt aacaatcatg
     1321 tttacaaata agtattttcc tacctttgta atagtagctg attgccatac tttatcccat
     1381 ccatttatga atttaaagtc aacattactt tcagttaatg ttgtaatgtc ttttaaattt
     1441 tctctaataa cttctatatg gctacctaaa tttcgtgtac tttcaaaact aacatttggt
     1501 atcgtaatac ctgagctaca aataaatccc gtttgattat catattgctc taaatttagt
     1561 agaccaagtt caacttctct tggtatttct aattcataaa ttatttttaa aggatttttc
     1621 tttaattttt ctttaaaatt agcaatgcta aaaggagata ttgaggtatc tactatatct
     1681 attacaccta ttccattttc tgttgatata gacaatgctg attcggtctg aacattaaat
     1741 atataatcta catgagattg cttgtataag ttagttataa acttagacat atctaatctt
     1801 aatacagata tatctaaatt taatctaaac tcatacattc catgcacttc tatccagttt
     1861 tcattaccat taaaaactat ttcttcacat ctttttatta gtttatagga accatttttg
     1921 taaactattt catctcttat tccatttgct aaacctctta atgtatgtgg aattgtcttt
     1981 ttatcatatt tatttccttt agttataatt gttttattcc cattatcatg taaatctttt
     2041 attatagtaa ctcttagata ctttgcattt ttaggacttc tagcaacata aaaaatatct
     2101 ttatctccta ttattctttc atttaatggt gctggtatga aacttttatt tatatcatac
     2161 caacaaatat ttctactaca attataaaaa gtatactctg tatctggttc tatttctata
     2221 tagtctaggg tatatttatg attactagct tcagaaataa gttcctctcc attaagatat
     2281 tgtaaaatat aattatcttt aaaatcggca ttcttattaa agatattaat tccactatat
     2341 tgataactta aaaattctat tttatctctt tgcccaacac ttaatagttc attagagtaa
     2401 tctatattaa tatctgtata atctccttct aatatggtaa ttttcaaact tacttcataa
     2461 ccagatgtaa gtgtatcgtt atctaaaaca ctccttaaaa cagcattaca atcacttaag
     2521 tctgatattg ttgtaaatgt atatttaaat ttaccaatta ctcctcctgg tatagctata
     2581 tttgtcagat taggaataaa tactgaattt tcttcgccta aactatgaat atatattcca
     2641 cttgtactcg gtaaagtgtt tttatcaaca tcaactatga ttgtatatat ggtatctggt
     2701 ttatatgaag tgtaattgat tgtaaaaaaa ttagaatatc ttatatcatt ttctgttagt
     2761 atatttattt ttccatctac aaaagttgct ttccataaag aaaaatctga actagttttt
     2821 ccccataaat caattaatgt ctttccttct atattaaaat tagttaaata gccctttttg
     2881 ctattttcta ttatatattc tcctgtatca gtactacatt ttatattgtc aatttctttg
     2941 agattttcta tatcatcaat tttattttct agaactttta ataaagcttc tatctttaca
     3001 gatgaataag tagttgtttc agtacttcta ttatcatcaa tagttgctga aatcatgtta
     3061 atagatgagt ttccatttac aactatacat tccaattcat tgtttaattc attaaaaact
     3121 tttatactat tatcatctaa aatttggtat gagcaaaata cattgttatt agtagattta
     3181 tctattaaac ttacaatcac ttttctagtt acaaggttat gctcaactat agctgtgtat
     3241 acattttctt ctaatatcca gttatttatt gatatatctt tagatacaga tacattagga
     3301 cctccctgaa ttaatttttc taaatcttct ttatttgctt tcttactaag ttctaggtta
     3361 atattatcct gctcattatt taatttatta atatttttta ctacatcttc atttaacaat
     3421 taatcacctt cttctaataa aagttatttt gtactattta gcataagcca tgcatcccca
     3481 cgctccagaa tctgaactct ctgtaaactt acatcctcct acacatatat attgtgcagt
     3541 tgctcctgtt ccttgatgaa taggtcctgt tgttgttgtt cttgttgcaa cttgtgaacc
     3601 agtttgtaaa tgacttattg ttcctgttgt attagtgcca ttactccatg aacctattgt
     3661 taaacatata aatatacatt gatttggtgt tgtactactt accatgcccc ctgtatcatt
     3721 tgaatttcta ctaccactat ctattacagc attagagcct tttgcaaaat atgtagtatt
     3781 ttgagtagaa gtgctaatat tactataatt agtttgtaag tagcttgaat ttgctagact
     3841 agttcttcca atttcagttt taagtatttt attaagcgca agcgggcttt tagaaataga
     3901 t
//
LOCUS       DAMJYC010000195         3889 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJYC010000195 DAMJYC010000000
VERSION     DAMJYC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.202"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..546
                     /locus_tag="PC472_003921"
     CDS             <1..546
                     /locus_tag="PC472_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2729189.1"
                     /translation="SHINILIIADIKNKDICLKLNIKYMFNLININRQLYPAENSKNN
                     DKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSVYVLINT
                     LYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKIMNKNKG
                     NKEGDSNESNRFDTESYGNNS"
     gene            500..928
                     /gene="ytfJ"
                     /locus_tag="PC472_003922"
     CDS             500..928
                     /gene="ytfJ"
                     /locus_tag="PC472_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2729190.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1176..1616
                     /locus_tag="PC472_003923"
     CDS             1176..1616
                     /locus_tag="PC472_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2729191.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1632..3083
                     /locus_tag="PC472_003924"
     CDS             1632..3083
                     /locus_tag="PC472_003924"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2729192.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNIATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3174..3842)
                     /locus_tag="PC472_003925"
     CDS             complement(3174..3842)
                     /locus_tag="PC472_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2729193.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFILSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1632 a          355 c          663 g         1239 t
ORIGIN      
        1 tctcatataa acatcttaat aatagctgat ataaaaaaca aagatatttg tttaaagctt
       61 aatataaaat acatgtttaa tttaataaat ataaatagac aactttatcc agctgaaaat
      121 agtaaaaata atgataaaaa agaggggatg aagaataata tagattcaag tatattatta
      181 gctgatgacc ttttgagcat ttatagatta ttaaaaaaga taaaaataca tgaactttat
      241 tcaaatatta attttggaac tggaaatatt ggcttaacat cttctgtata tgtattgata
      301 aatactttgt atggaaattt atttaatatg attgatgcag aaaaaatgta tttaaatgta
      361 aatccagatt ttacaaaaga ttatgtttta ggaaatataa ggatacatat aaggccaaga
      421 ataaaagctt tatttaatat tataataatg attaataaaa ttatgaataa aaataaagga
      481 aataaggaag gtgatagtaa tgagagcaac aggttcgata cagagtctta tggaaacaac
      541 tcttgaaaca attaaaggtt ctatagatgc aaatactatt ataggagacc ctataaaaac
      601 agatactaca gtggttgtac ctatatcaaa ggttacaatt ggctttggta taggaggagg
      661 agaatactca aaaggatatg atgataaaga tagggaaatt gaattaaaaa atgaaaaatc
      721 agatacgaat tttgctggag gtagcgcagg ggctatatct gtacaaccag tagcatttgt
      781 agtagttgaa agtggagaga ctagaataat gagtttagat agtaatatca atctagtgga
      841 taatatatta tctataactc ctagagtatt ggagaaaatt caaaatatat cacaaagtaa
      901 taagaatcaa gataaaaaca atatgtaaga ataaaatatt tttaaatatg tatatagttt
      961 taagtaaagt agtggggtta atataaatcc cactattttg ttgtgtactt taaacattta
     1021 tatctaataa gattttagtt taaaacaatt taaatagata ataaagttga tatatagtat
     1081 aattataaaa agaaaagaag tattatatta tagatatata aacgcatttg agatataaga
     1141 gggagttata aatcattagt tttgaggtga atgttatgag cgataaagaa ttatctattt
     1201 tgttgttaga aagtatgaat aattttcata atttaataaa gattattaat aacgaaagat
     1261 ataaaaaaga taaggttctt acagaaagac agttctttgc cttagtaaaa ataagaaaac
     1321 atgataaaat agagcttaaa aatctaagta gagatttgca tgtatctact tctagtctgt
     1381 gtatactact taataaattg gttgagcaag agtatgtata tagggaagaa gatagtagag
     1441 atagacgaaa tactttttat ggaataacta aaaatggaga aaagatattg gataatgaaa
     1501 tactaaagtt tgtatctata ataagtgata aaatggattg cctagatata gataataaag
     1561 ataaattatt tacttctttg gaagagtcta aaaatataat agaacaatta ttttaaaaag
     1621 gagaatttat tatgaatata aagtattcta aagagatatt aaaaagttta gaaaatgcta
     1681 taacagtagt agctctattt gagggagaag atgtttttac gacatttgat aaaataaata
     1741 tgataagcag aaattttaat tctaaagaaa atagtataga tactgtaaca ttggttgaaa
     1801 ataacaattt ggtaaatgtt tgtcttgttg gttttggtaa gagagagtta ttgaatagag
     1861 aaaaaattag aatcataggt gggaatttgt gtaaaaaatt aaaaaatata ttgaaaaatg
     1921 aagcatataa gagtagtgat gttaatgtac tgaatcttaa tttaaatagt gatgaaatag
     1981 gttgttttgt agaagggata ttacttggaa attataagtt tgataagtat aagacaaaaa
     2041 gtaaggattt tgaaagaaaa gaagttgata ctataactat atttactgaa aaagaagttg
     2101 agtgtcaaat agaaaaggct aaaatattag ctaattcaac tataatagct agaaatctag
     2161 ttaatgaacc atcaaacgta atatatccaa aaacattagc agtagaaaca gttaaacttg
     2221 gtgcagagtt tgggtttaat gttgatgttt atgaagaaga aaaaataaag gcattgggaa
     2281 tggatgcttt tttcgctgtt tcaagaggtt caattaataa accaagattc atagtaatga
     2341 gatactttgg agataaagaa agtacagata tattaggtct agtaggtaaa ggattgactt
     2401 atgatactgg tggatattct ttaaaatcaa atgcttctat gctagatatg aagacagata
     2461 tggctggtgc tgcgagtgtt ataggtgcta tgtgtgctat atctcaaagt aaattaaaga
     2521 aaaatgttat agcagtagtg gcagcatgtg agaatgcttt atcaggaggt tcatacaaac
     2581 ctggagatat aattagttct atggcaaaga aaactattga agttttaaat acagatgcag
     2641 aaggtagact tacattagct gatgcaatat actacattat aaacaatgaa aaagtaacaa
     2701 aggtggttga tgtagctaca ttaactggtg ctgctttaac attgttaggc aatattgcaa
     2761 ctcctatagt aacaaataat gatgattttt attgtgaatt agaaaaagct gccactttat
     2821 caggggaaag ggtatggaag atgccaattt atgatgaatt taaagatatg ataaaaggag
     2881 aagaagcaga ccttaaaaac actggtggta aaaatgctgg atgtataaca gcaggagctt
     2941 ttattggtga atttgttggg aatactcctt ggatacatat ggatatagcg gggacaagta
     3001 catcatcaaa atcaattgga tacaaagcaa agggggctac aggagaacct gttagaacat
     3061 tgtactattt ggcagaaatc taaaaaacaa ttataaaata taatttataa agccacatgg
     3121 atatttttgt aatttaagat tatctatgtg gctttatttt ttatttaaat aaattattta
     3181 ctctttttct ttttttcaag tataaaagta tctctattaa ataaaagagc aggtatgtac
     3241 ataatactag taacaaaaat aaacgtaaaa atagcagttg tgctatgact taaaaaacca
     3301 taatagtacc tagagttggt aaatgtgttt agtaaaaata tgaatgaaaa ataaataaaa
     3361 catatagcta agttatctct aagtgcatat ctgttaactc taaattttct aacatcaacc
     3421 atatatatat ctgcataaat tatcaagcac ttagataaga taaagataaa atatattaag
     3481 ctaggaaatt catagcccaa gtttttagaa aaaatgagtt ctccaactga acaaactaaa
     3541 ctccaagaaa aaaatatatt aaaaagatga tactgcttaa aaattagtat ggctatacac
     3601 agatatatac aaatataatt gattccaatt ggtaaagagt ttagagtact ataattacct
     3661 gaagaaataa gtaataattg ctctagagcc ataattatta caagtaatgt acaaattatc
     3721 ttctcaactg tatagctata aggtaaaata tttttggtta gttttggaca atagtataaa
     3781 aataatccaa atataaagag acttacaaat tgttcactag aaaaaagaaa aattttaaac
     3841 atataacatt ctctcctata ataaatttaa tatgtatcta tataaatat
//
LOCUS       DAMJYC010000196         3854 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJYC010000196 DAMJYC010000000
VERSION     DAMJYC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.203"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            91..1203
                     /locus_tag="PC472_003926"
     CDS             91..1203
                     /locus_tag="PC472_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2729194.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1411..1887)
                     /locus_tag="PC472_003927"
     CDS             complement(1411..1887)
                     /locus_tag="PC472_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729195.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2086..3711)
                     /locus_tag="PC472_003928"
     CDS             complement(2086..3711)
                     /locus_tag="PC472_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2729196.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1344 a          615 c          428 g         1467 t
ORIGIN      
        1 tattaaaatg tatgcaactc tatttcaaaa ttattttgtg atatatatat gttataataa
       61 atgtataaaa ttttgattgg tgggttatat atgaatgcat ttattagaaa aagaaataaa
      121 aactatgttg tttacctgga atttgaagat gaaaagacag gtaaaagaaa acaaaaaaat
      181 atgggtactt ttgataaaaa aagggaagca agtaaaagac taaatgaggt aaaagaaagc
      241 ttatataatg atggtttttt agtaccaagc gaaattacag tgtcagaatt tctattagat
      301 tttctaaata aatatagtga aaatatatct ttagctacat ataataatta tgttagtatt
      361 tgtaagaatt acattaatcc atctattgga aaatataaaa tacaagactt acatccggtt
      421 cacatacaaa actacataga taaactatct tataaactaa atcctcaatc aattaagatt
      481 catataaata tattgaaact tgcaataaaa agagcttata gactcaaatt aataaaagaa
      541 aatgttatgg atagtataga ggctccaaga tataaaaaat ttaaaaatga gatttatgat
      601 agagaacaaa tgataaaatt attagattta gcaaaaaata cagaaatgga attacctatt
      661 aatttggcta ttggattagg tcttagaatt tcagaagttt taggtctaac ttgggataat
      721 attgattttg aagaaaatac aataacagta aataagataa cttcccgaat taatggttct
      781 gttattctca aagagccaaa gacagaaagc tctgttagaa aaatatcttc tcccaaagaa
      841 ttaatgtctt tattaaaaga atataaaata aaacagaata aaaatttatt aaaatcttcc
      901 attagaaata ataataattt gttgtttttc aataagaaat gtgaacctat tgctgaagat
      961 gttatgagta aaaaatttaa aagattttta gaaaagaatg aattacctca tattagattc
     1021 catgatttaa gacactcaca tgttacttta cttataaatt ctaaagtacc tataaaggtt
     1081 atatccgaga gagtaggaca ctcaaatata agtactactc taagcgttta ttctcatgta
     1141 cttaaagaaa tggataaaga ggcttctgac aaaatttctg aaagtttatt taatgctaat
     1201 taatattaat ttatcttagc tttaaattag cttagcttat tgagatagat tcattaatat
     1261 taaatgtatc tatctcaaat taataactac ttaaataatc tgttttattt taatttatcg
     1321 cctcagttat tatacttttt tacgaaaaat tatactttac aataaaaaag atattccctt
     1381 atagaaaata tctttttaaa tactatatta ttatatactt gctaacattg gaatatctaa
     1441 catcttcttc attacatcat ctactttttt tgctttatat tcattgtatc caattaaata
     1501 tcctttttca tatgaacttt ttaaaatctc tttttcatat aaattatcag atttggcttc
     1561 tgtgacactt aaattataaa gccctttaca cccatcttca taacctttat tatacccttt
     1621 ttctgcaaaa tagtttttat gatttttttc gttatgtact gaagatattg ttactccaga
     1681 attttctttt gtactagcat aaggacaaaa acctttattg tgttgatgtg ctgaatgtcc
     1741 tccacaataa taacgtgtta actctgattt atttttatta tgatgacctc ctgatgaatt
     1801 atgccctcct gagtgagcat atgaaaaatt ccctacacta atcatagcta aactcaatac
     1861 tacagctact aaccttttct taaccataat ctattccccc caaaatgtta tatattttta
     1921 ttatatcatc tactatatta tatttaaatt cttaatatac aaaatattac tctattctgc
     1981 taataaacat gtttaaacaa cataaaaaac ctcctaatat ttgattaaaa ttttacacac
     2041 tcatgcatag catgtctcaa atattaaaag gttttatact aataattatt tatcttttcc
     2101 atacatttta gctaattggt cttgtatctc tttactttct aaatactcat caaagtccat
     2161 ttttctatcc attattccat ttggagttat ctctataatt ctatttgcta tagtagatat
     2221 aaactcatgg tcatgagaag tgaataaaag tactcctggg aacttttcaa gtcctttatt
     2281 tacagaagtt atactttcta ggtctaagtg gttggtaggg tcatctaata caagtacatt
     2341 tgcatttgaa agcattagct tactaagcat acatctaacc ttttctcctc cagatataac
     2401 ttgagcttct tttaatgctt cttcacctga gaataacatt ctacctaaaa atcctcttat
     2461 aaaactttca cttttttctt cagagaactg tctaagccag tcaaccaaag aatattctac
     2521 tccgtcaaaa aacttattat gattttttgg aagataatct tgagaagtag ttactcccca
     2581 tttatactct ccactgtctg gttcaagttc tcccataact atgttgaaaa gtgcagtagt
     2641 agctatttca tctcccataa atactacttt atcatctcta tccaatctaa atgacacatt
     2701 atcaagaacc ttaaccccat ctatagtttt agttaaattg tgtacttcta aaacttcatt
     2761 cccaatttct cttgcaggtg taaatcctac atatggatat cttcttcttg atggttgtat
     2821 atcttcaact tctaatttct ctaactgttt ctttcttgat gttgcttgtt tagcctttga
     2881 agcattagaa ctaaatctag ctataaattc ctttaattga gctatctttt cttcagtctt
     2941 tttattttgg tccttagcca attgtaaagc caattgactt gactcatacc agaaatcata
     3001 gtttccaacg tacatttgta ttttaccaaa atctacgtca actatattag tacatatttg
     3061 atttaggaaa tgtctatcat gtgatactat tataactata gattcttcta aatccattat
     3121 aaaattattt aaccagttta ttgatttaaa atctaagtgg tttgtaggtt cgtccattaa
     3181 tagtatttca ggcttaccaa aaagtgattg tgctaacaat accttaacct tttccccacc
     3241 aactagttcc ttcatttgtt tgtaatgcat atctttcgtt atgccaagac ccataagtat
     3301 tttctcagca ttagtttctg catcccatcc gtcaagctct gcaaactcac cttcaagttc
     3361 ggcagctttt atcccatctt cttcattaaa gtcttccttc atatacaatg catctttttc
     3421 tttcattatg ttccaaagtc tctcatgacc tcttataaca acatttaaaa cagtttcttc
     3481 ttcatattca aagtggtcct gttttaaaac tgacattctt tctttatcag ttatagatat
     3541 actacctgta ttaggctcta tttctcctga taatattttt aaaaaagtag attttcccgc
     3601 accattagct cctattattc catagcaatt acctttagtg aattttaagt taacatcttt
     3661 aaatagttcc ttatcaccaa atctaagtcc aacacctgta acttgtaaca ttaatatatt
     3721 ttcctttctt tttgctcata atttatttta tcataaaact ttgataacat ttttattata
     3781 tattatcacg taaatagtat attttttata ctaaatttgt gaaatattta ttaaaataat
     3841 actaaaaatt gtaa
//
LOCUS       DAMJYC010000197         3698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJYC010000197 DAMJYC010000000
VERSION     DAMJYC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.204"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            200..1780
                     /locus_tag="PC472_003929"
     CDS             200..1780
                     /locus_tag="PC472_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2729197.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRLSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1954..2400
                     /locus_tag="PC472_003930"
     CDS             1954..2400
                     /locus_tag="PC472_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2729198.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2421..3464
                     /locus_tag="PC472_003931"
     CDS             2421..3464
                     /locus_tag="PC472_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2729199.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1496 a          343 c          692 g         1167 t
ORIGIN      
        1 aaatcaacat aataacttaa attatatggt ataataaatt aaataattgt tttaattcaa
       61 aattttttgt tattttaaat tatttcaaaa ctcttagttt tagtgatttt atttatgata
      121 tttatgggat taaaaagatt tcaatagata tttttggtaa gtatttaaga agtctttaac
      181 tttataggag tgataattta tgctaatagg tacatctaaa ttaagcagaa atgttgatac
      241 tgcatgtgta gaagaagtag ggattccaat gattgtattg atggaaaatg cggttatatc
      301 tgcaatgaaa aatatggata tagacatgta taatagttat acagttgtat gtggtgttgg
      361 aaataatgga ggagatggtc ttggaatagc aagacatcta aatacatatg gaaaagaagt
      421 aaacgtattt ttagtaggta atcttgaaaa actaagtgaa tgctctaaaa taaattacaa
      481 tgtactttta aatatgggaa ttaatattat aaatattaat aaaaatgatt taagtgaaaa
      541 aaattatgga gatgaaaatg aagtagataa aattattaaa gttgagaaac aaaatattat
      601 aaaaaataaa aatttaacaa tttttaaaga tagactatct aaaagtgatg ttgtagtaga
      661 tgctatattt gggactggat taaaacgtga tataactggt atttttaaag aagttattga
      721 catagtaaac gataatagca aaaatacata ttctatagat attccttcag gtatagatag
      781 cgataatgga aatgtattag gaacttgtat tagagcaaac aaaacgatta gctttgagtt
      841 ttataaaaga ggttttctaa actatgacac tagcacactt ataggtgatg tagttgtaga
      901 aaagataggt gttccagatt ttattacaag aaaataccat gatgaagagt tcataactga
      961 taagactttt gtaaaaaata atattagaag aagagataag catggtttta aaagtgattt
     1021 tggaaaagta tctatagttg caggttcaaa aggattttat ggtgcaagtt tcatagcaac
     1081 tgaatctgct gttaagagtg ggagtggact tgtaacatta attagtagtg aagatgtgca
     1141 agaaaaggta tctattagac ttacagaagc aatgacttta aattttgaag aagaaagatt
     1201 aaataaatta cttaattcta gtgatgcaat tggttttggt ccaggtatgg gagataatag
     1261 tcaaacattt gataaattac ttaagatagt agaaaattct aattgcccaa tagtattaga
     1321 tgcagatgga ttgaacgtta tgaaggacag atgttataag tttttagaat ggaaaaatag
     1381 atttgtaatt acacctcatt tgggagaaat ggccaggttg acaggagatt caattgggta
     1441 tataagagaa catagagtag atgttgcaaa agaatttgct caaaaatata atctggtggt
     1501 gcttttaaaa ggttatcaaa ctgtcataac agatggtaaa aaaacatata taaatcctac
     1561 aggaaatagc tgtatggcaa ctggtggaat gggtgattgt ttgcttggaa tgattacttc
     1621 atttataggt caaggtatgg atatattgga agctacagtt agtggagcat atatacatgg
     1681 ctatatagga gataaattga gcaaagcaat gtatacggta aatgctacgg atttaataag
     1741 taacatttca ttaacaatga aagagttgtt ggatatataa ttggtgatat ttgatactac
     1801 tttgtaacaa ttttaacaaa gtatgttgat ataatactat taattaatta atttatttta
     1861 ttgttttgta atttaaattt atattcaaaa acattgtata aacatataat ttatataggg
     1921 ttttataatt catatgaaaa gagggtagat agtatgaagt taatatggtt gatagtagca
     1981 gtcttatttg gaatagcaga aatgttaaca ccaagcttaa cattgatatg gtttagtgta
     2041 ggagctgtaa tacttatatt tttaagtagc tttatagaga gtattttctt acaaatttta
     2101 atatttgcag taatttctat agcaatgtta gtcgtagcta ctaaaaaaat agtaaaaaaa
     2161 gataaaggct ataaatctaa cactaattta caagcaatga tgtctaaaaa aggtattgtt
     2221 acagaagaga tttctccaaa taacacaggt ctagttgtag ttgaacatga aacatggacg
     2281 gctatttcta tagatggtga aaagatagaa aaaggttcta cagtagaagt attaaaaatt
     2341 gaaggggtaa aattagttgt taaaaaagta gacgccacag ttaatgtgac aaaccaatag
     2401 aataaaggag gatttatagt atgggtacta aaattgtttt aagtattgta ttaatagtag
     2461 tagtcgtagc aataagttta acttgtataa gagttataaa acaatctaaa gtaggtataa
     2521 taatgagact tggtaagttt caaaaagtgg ctgaaacagg tgtacatttc ttaataccat
     2581 ttttagataa aatggcatat gtaattgacc ttagagaaat tgtaatagat tttccacctc
     2641 aaccagttat aactaaagat aatgtaacta tgcagattga tacagttgta tattataaag
     2701 ttacagaccc agttagatat gtgtttgaga tagctaatcc aatagctgct attgaaaatt
     2761 taacagctac aacactaaga aatataattg gtgaacttga tttagatgaa acattaacat
     2821 caagagatat aataaatgta aaaatgagaa ctatccttga tgaagcaaca gataaatggg
     2881 gtataaaagt aaatagagta gagttaaaaa atataatgcc tcctcaggat attcaagttg
     2941 caatggaaaa acaaatgaga gcagaaagag aaagaagaga ggcaatactt caagcagaag
     3001 gtaataagtc agctgcaata cttcaagctg aaggtgaaaa acaatctgca atattaacag
     3061 cagaagccaa aaaagaagct atggtacgtg tagcagaagg tgaaaaagaa tctgctatat
     3121 tagtggcaga aggtgaagct gaagctataa gacagacagc tattgctaag gcacaaggtg
     3181 aagctgaaat gataaaaaga actcaaatgg caacagcaga aggtttaaaa ttagtttttt
     3241 cagcaatgaa agaggctgat atagacaata atattctagc attaaaatct atggaagctc
     3301 ttgaaaaaat ggctgaaggt aagtcaacaa aactggtttt accttcagaa gcagttaatt
     3361 tcctaggaac attcaaagga ataaaggaag ttatgagtga tgataacaaa gaagtacttg
     3421 atataaaaga agttttaaat gataatgaat cattaaaaaa ataaaagagt tttgttatca
     3481 taggaaggat atggaaatta aaggtcgaat taattaaata tagcacaaaa atgcacgttg
     3541 aaaagaagag gacttataaa tttttaagtc ttcttctttg tttttattag aaagatgaaa
     3601 ttataaaaat aaattaacag aatctttata caatttatcc tttactaatg taaaaagtgt
     3661 tatataatat tatataaaac atatagcaaa taagcatg
//
LOCUS       DAMJYC010000198         3677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJYC010000198 DAMJYC010000000
VERSION     DAMJYC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.205"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            97..603
                     /locus_tag="PC472_003932"
     CDS             97..603
                     /locus_tag="PC472_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2729200.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            731..1747
                     /locus_tag="PC472_003933"
     CDS             731..1747
                     /locus_tag="PC472_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2729201.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2101..2805
                     /locus_tag="PC472_003934"
     CDS             2101..2805
                     /locus_tag="PC472_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2729202.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     VGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3100..3579
                     /locus_tag="PC472_003935"
     CDS             3100..3579
                     /locus_tag="PC472_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2729203.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1517 a          336 c          626 g         1198 t
ORIGIN      
        1 taaaatcttc ctatatcata taaaattaag tattgattag tctattacat aaatatagaa
       61 tataaatata tccatttaat aataaggaga agagatatga aagagtatgt agttgtggat
      121 ttagaaacta caggattaga tccatataaa ggttgtgaaa taatagaaat aggaataaca
      181 gaaataaaaa atgaacaaat tgtaaaaaac tattcaaggt tgataaaacc taaggggata
      241 atttcttcat ttataacaga attgacacat ataagtaatg aaatggtaga aaatgaagaa
      301 ccactagaat tagttttacc tagatttaga aaatacatag gtgatagaac tataattgca
      361 cacaatgcga aatttgattt aaaattttta aattactatc ttagaatgtt agatttagaa
      421 ccaataaaca attatatatg tacagtagag ttattaaaaa aatgtaaaag ttataaagga
      481 aaaaacaaga aacttgaaac agcttgtgct tattacaaca tagagaatat aaatgctcat
      541 agagctgaca gtgatacatt agcaactgca aaactatttt tgaaaataaa agatgaatat
      601 taattataaa tatagagaaa attagtgaaa atatccttta taaaattgcc ccttaagctt
      661 acaattttgt tctttgattt attttgtaga acaatgtata atgtacctta tatgaagaag
      721 ggagactaaa atgggttatt acaatgataa tgataataat aaaataagac gtaaaaaaat
      781 tagtaacaac aaagatagta ataaaaaatc taaacattct tatacttcgg caaataaaaa
      841 aaataaaagt cctaatacaa gttccagtaa aagaatgagt gaaaaagtag attcacagaa
      901 tcttataaaa agagatagag atagttttac aagagataat gtaagacctt taaatacaga
      961 ttacaatcaa agagaaagaa gaagagtcga gtataaacaa agagagaaaa aattaaaaag
     1021 taaaaaaaga aaaaaacaaa aaaggattag aatagttagg gcattattat ctattatgtt
     1081 agttgcacta attatatttt cagtggcagg tgcaatattt acagtttcag ctataaaagg
     1141 gtcaccagaa gttactaaaa agttaattag gtctaaatat ataagtagtg aggttgtaag
     1201 tataaaacaa atgccaaatg atttaaaaca tgcaatagtt tccatagaag atgagagatt
     1261 ttataagcat aaaggagtag atgttatttc tctagttagg tcagtattaa acaatgtgtt
     1321 tacagataca acacaaggtg caagtactat agatatgcaa gtttcaaaaa acctgcttac
     1381 tagtaatgat aaaactatga agcggaaaat aagggatatg tataatgcta agcagatgaa
     1441 taagattatg actaaggatg aaattcttga agcatattta aataatatgt atctaggtaa
     1501 aaatgcatat ggagctgcaa agggagctga agtctacttc ggtaaaaaag ttagtgaatt
     1561 aaatttagct gagtgtgcta tgttagctgg tatgacaaat aatcctgcaa ggtatattga
     1621 ccatggagag gctaagaaga gacaagtgat tgtactgtat aagatgcatc agcttggtta
     1681 tataactgat aaggagtata gaatagcaaa agctgatgac actccattta aatctgaaat
     1741 tgattaaaat tgtaagaata tagtattttc aatagtttaa acaaacgaaa atgtaataaa
     1801 aaaggtgatt tgccacccat ttgccaccat aattataatt taatagcaaa ttgttttaat
     1861 gtacaaatca gataatataa tattgtttta aatactacat tatgctaaaa tttaattgta
     1921 ttttgtaaat taaaaaactt ataatttagt tttctagggt tccgcatttt ataatggtca
     1981 ggtccaagag aaaacgcaca attgttgtgt acacggaagg ataaaagcct gggagatatt
     2041 taataatatt tctataggct tttttatttt tacatatttt aaatttgagg aggttttcta
     2101 atgaaaaaat ttgttattga ggatgcattt tggaatctat ttcctagtgc aaaaattgga
     2161 gttgttattt gtaatgatat agataactct ataaaagatg aagactataa gaaaataatt
     2221 ttgaagggag aagaagaagc attaaaatac ttggaggact cagaatttag caacaaccaa
     2281 gtgattaaag tatggagaga agcatttaaa aaatttaaaa cgaaaaaagg agcaaggtct
     2341 tctattgaag cattattaaa aagagttcat aatggaaata atataggaac tattaaccct
     2401 ttagtggata tctacaattt tatctcatta aaatatgcat taccttgtgg aggggaggat
     2461 atagataaat ttataggaga tattaggcta actagagcaa ttggtaatga ggagtttatc
     2521 ccactaggga cagatgaaaa ttcatttcca tatgaaggag aaataattta taaagatgat
     2581 gttggagcga tttgtagatg ttggaattgg cgtgaagcag tgagaacaat gcttactgaa
     2641 gatactaaca atgccttttt atgcattgaa ttagtagatg aaagtagatt taaagaattt
     2701 gaaaatgctt taaatgaatt agctaaaata gttcaagaaa aattgggtgg gaattgtaaa
     2761 atttcaattt tagatattga taataaagag gtatcagtgg attaatatgt aggtgctgtt
     2821 gtaatggact attctagtta taaagatact tatatttagg gtaagaaggt ctattttaga
     2881 tataacgatt atcaaaggat ataatataga aaataaaaat gtagaatata aaaagccttt
     2941 aaaataagaa aaaaatgtat tttaaaggct tttattatac tcttttttat ttgcataatt
     3001 aacaataaaa atatattgtt aattataata tttttattga aaaacgatat aatataatgt
     3061 attatactat atataaaata ttttaaagga gttggcatga tgtctaataa acttataaaa
     3121 aatttactaa gtggtatatt acaaatttta ttttttcttt taggattagt aattgttgca
     3181 ggaggattta aaagttttat gtacctctgt tttagtggag aagcaactct acaaggtact
     3241 atttatggaa tattaatgtt tattttaggt gtatcatatt ttatcattat aaaatcattg
     3301 atagaagtat taggtagttc tgaatatagc ctatttgtaa aagagaatgt aaaaagattt
     3361 aggattattg gatatctttt gcttttaaac tctctaattg agtttatttc aacttttggt
     3421 actactggaa aaggaatgag atttctagat ttaggatttg gattttattt tactgttcca
     3481 gtatttgtat attttataac ttctcttatg agttttgtaa ttgcagatgg atttgtaaag
     3541 gcaataaaga taaaagagga taatgattta actatttaga tgtgacactt taatatatag
     3601 gagataaata acccgaaaag agtgactaac tagtaattta aaataaaata tattagggta
     3661 taatacataa actataa
//
LOCUS       DAMJYC010000199         3675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJYC010000199 DAMJYC010000000
VERSION     DAMJYC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.206"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            248..1624
                     /locus_tag="PC472_003936"
     CDS             248..1624
                     /locus_tag="PC472_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2729204.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1935..2606
                     /locus_tag="PC472_003937"
     CDS             1935..2606
                     /locus_tag="PC472_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2729205.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2795..3592
                     /locus_tag="PC472_003938"
     CDS             2795..3592
                     /locus_tag="PC472_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2729206.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1392 a          407 c          682 g         1194 t
ORIGIN      
        1 atagtaaaag ctatgatagt tttagaccta ttatatgtaa aaaatataga tgtttttggt
       61 gcaatttaaa ttaaaagata tatataaaaa tttaaaatat tccaaacaga aattataaaa
      121 attactatca ttgtctttat acaatattta tactataata gaataataat ttagaggaaa
      181 gctctaaaaa taaacaaatt aagggtctta acagtagtga ttttatgtta aggaggtata
      241 aagaaagttg aaaacaatag ttagaagact tattctctac gcagatggaa tgcgtcctac
      301 tcaaataatg gtatctgggt ttgctgcgat tattgtaatc ggagcattgt tgttaacact
      361 cccgatagcg tcgcagagtg gagagagtat aggcttatta aatgctctat ttacggctac
      421 ttcagcggtt tgtgttactg gtctagttat ggtagataca gcaacttatt ggagtttatt
      481 tggacagata gttataataa cattgataca aattggtggt cttggattta tgaccgtagc
      541 cacaatgttt tcacttatgg cacgaaaaaa aatacaacta agagaaagat tattaataca
      601 agagtcatta aatcaagctg atttatcggg acttgtaaga cttacaagat ttgtattaat
      661 aataaccata acaatagagg gaataggtgc actggtttta tcaactgtct ttattccaca
      721 atttggtttg tctaaaggga tatggtatag tgtatttcac gcaatatcag cgttttgtaa
      781 tgccggattt gacttgatgg gcagtgtaag tggcccattt acatcgttaa attcttatgt
      841 aaacaacttt acagtatcaa tgactgtttg cgcacttata gtgcttggag ggctaggatt
      901 ccctgttgta cttgatattg taagaaaaag aagattttca aaattaaatg tgcattctaa
      961 ggttgtattg ttttcaactg ctacacttat ttttgtgggt gcattattta tttttcttat
     1021 agaatttaat caaaaagcta ctatggcgga cttgccttta aagggaaaag tactatcagc
     1081 aatatttcaa tcagtaacag caagaactgc tggttttaat acgcttgatt tagctacttt
     1141 acgagaaagt agtgtatttg taatgataat tttgatgttt attggagcat cacctgcatc
     1201 aactggtggg ggtataaaaa caacaacact agctgtttta atcattacag ttagaagttt
     1261 tctatcagga aaatcagata tagaagcttt tgaaagaaga ttagctccat caactataaa
     1321 aaaatctctt ggtatatttg taatcagtat atcagcagtt atttttggaa cattgattat
     1381 ttcaataact cagcctaatt ttacattagt tcaatcagct tttgaggtta catcagcact
     1441 tgctacagtt ggttcaagtt tagctggaac tccaaactta aatgctttag gtaagattat
     1501 aataataatc tttatgttta tgggtagagt tggttctctt actttattta tggctatact
     1561 ttctggtggt agaagaaaga gtcaaccaat cagatatgca gaaggtaaaa ttatggttgg
     1621 ttaagttacc aaggagtagt ataaatttat aatgggttta gagtagcact gtaaatctgt
     1681 tgataaaata aatgagccta gatgtaatta tatgaagaaa taaaatcaac taatgaacaa
     1741 aattaattaa tgaaataaaa aaacagggaa ataaaataca aataaaaagg taaatcaaaa
     1801 ttaaattaat ctgtgtgtat aaaattatat attgtgtata agttttgtat gtgtaaagta
     1861 tataagtttt gtatgtgtaa acaattaatt taagagatat tatataaatt gtgtattagg
     1921 gaggaagttt cgagatgaag caatatatag tcattgggtg tgggagattt ggaagttcag
     1981 ttgcgtctac tatgcatctt ttaggacatc aagtaatggc aatagacaaa aatgaagatt
     2041 cagttcaaag tatatctgac aaggtaaccc attcacttat agtggatgtt actgatgagc
     2101 aagcgttaag gtcattaggt ttaggtaact ttgatgtagc agtagttgca ataggttctg
     2161 atataagggc atctataatg gcgactctta tagccaaaga aatgggtgta gagttgataa
     2221 tatgtaaggc aaaggatgaa ttacaagcta aagttcttta taaaattggt gcagatagag
     2281 ttgtatttcc agaaagagat atgggagtaa gagttgcaca caatttagtt tcggataata
     2341 tattagacca tattgaactt gacccagagt attcaattgt tgaaatcgta actccaaata
     2401 gttgggttgg caagacactt atagagcttg aattaagagc tagatatgag ataactgtac
     2461 ttgctataaa aacaggtaaa aatataaatg ttacaccttc tccagatgag gaacttacag
     2521 ccggaagtat cctagttata atcggtcaaa atactagtat aacagcgata acatctggaa
     2581 ataaggggat aattagaaga agataattta ctatttaata tatatttaat tgcaatgaaa
     2641 gtagatagta tcatataatt atgaatagtt atatgatact atttttttaa tcgaaggtag
     2701 tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg ctgtgctaat
     2761 ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa acagtaagga
     2821 taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga ataaagagtc
     2881 aaagttcata atagaaggat acagaatagt aatgcttgca cttgaatgta tggcaaacct
     2941 tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa aactattaga
     3001 agatttggat aaaaaaaaca caaagatata caagactact aataaaaact ttaaagaatt
     3061 agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga aaaaaaaatt
     3121 aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagacagaa tacaagaccc
     3181 aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg ccataatagc
     3241 actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta tgggttctat
     3301 ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc ttaaatcatt
     3361 ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca aaatagatta
     3421 tggctcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg aagaacttgt
     3481 atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt cgttaaatgc
     3541 tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt aatgtattgt
     3601 aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa aaatagtcaa
     3661 tatatattaa aaaaa
//
LOCUS       DAMJYC010000200         3457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJYC010000200 DAMJYC010000000
VERSION     DAMJYC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.207"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            306..953
                     /locus_tag="PC472_003939"
     CDS             306..953
                     /locus_tag="PC472_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2729207.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNKALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1120..1689)
                     /locus_tag="PC472_003940"
     CDS             complement(1120..1689)
                     /locus_tag="PC472_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2729208.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1694..2593)
                     /locus_tag="PC472_003941"
     CDS             complement(1694..2593)
                     /locus_tag="PC472_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2729209.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2604..2753)
                     /locus_tag="PC472_003942"
     CDS             complement(2604..2753)
                     /locus_tag="PC472_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729210.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2746..3330)
                     /locus_tag="PC472_003943"
     CDS             complement(2746..3330)
                     /locus_tag="PC472_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2729211.1"
                     /translation="MKRFFIVVLGCIFVLGVISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1228 a          577 c          381 g         1271 t
ORIGIN      
        1 taaaatttaa acaagttatt cataaaataa aataataatc atctataacc cacaacttta
       61 ttttatatta aataaaaagt attagtgatt atgttttata agtatttctt tattcagtta
      121 tttattaaaa actaatagta aatcttatac taatcgcata tttatgttat aactatattg
      181 taactatttt cgtaatctta ggaaaagatt acttataaaa ttaaccttat acaataatat
      241 atcgtataat cttaattagc ataatagtta caatattaaa atttaaatat gggagtgatt
      301 ttagtatgat aagacaaaca ccattagaga aacgatatga caagctaggt cctcatattg
      361 taacagcact caaaaaacgt tattttgatg cttattactg taaaactaga aataaagctt
      421 tgcaacaaat tttagattta attcctcaaa atcatgtagt atcatgggga ggatctgaaa
      481 cacttaagga aatgggcgta caaactgctg ttagagagaa aggatttaaa gttattgata
      541 gagatttagc aaaaactcca gaagaaagaa ctgaaattat gcgtcaagca cttctatgtg
      601 atactttttt gatgagtagc aatgcaatta gtgaagatgg acaactcttt aatattgatg
      661 gaaatggcaa tcgtgtagct gcaatgatat ttggtccaaa aaatgttata gttgtagctg
      721 gtatgaataa aattgtaaaa aacattgatg atgcaataca aagaactaga acaattgcat
      781 ctccagcagt tgttcagcgt tttcaagatg tcaatacacc atgctatatt aatggaagtt
      841 gtatagattg cacaagccct gaaagcattt gtgcatacat ggttactaca agaatcagtc
      901 gtccagcaaa aaaaataaag gtaatattgg taggagaaaa tcttggtcta taaaatatac
      961 atacatgtaa atagctctat aaataataaa atcatatgaa ataacttatt tctaaaagtg
     1021 gtagaataaa agcttctaca agactttcat tctaccactt ttatattctt ttaatttgtg
     1081 tttctatatt attaattgct ccgattttca tatatattac taactatttt tattttcata
     1141 atttttttgt tcttcatcaa gctgtttata ataatcttta ttatagtcag ctagataatc
     1201 tataccacct cctgtaatca catttaataa tttcattgca gattcttgat taactttaca
     1261 aacataatta tcaccagatt taacaagctc taatgtaata ttaaattctt ttttctttgc
     1321 agaatcaata tctttcatag ttggatttgg catcatttct cctagtttag gaggattctt
     1381 ttttaaaata gaaaccatat cagtattgtt aatttttaaa tctacagtca ctttatcctt
     1441 attattcttt atatctgaaa tttcccaagt catattttta gaatacattt taaatccttc
     1501 tttatcttcg tatataagat tacctaaccc tgattttggg aaatacttat caacagtatc
     1561 tgcatcttgt gtttttattg aattaaacat actatccact tcatttttta tcttattatc
     1621 attagtacaa ccagctaaca ccagtactga tattgcaaac attataatac atataataaa
     1681 tttcttcatt aaattatatc cccctatact tttttgcaca aatattattc tcttttttat
     1741 cttttgaaaa agttgtagta attgcatttt gtggacaaac ttttatacat tcaccacacc
     1801 taatacagtc tggactattt acatctttat atattggtat atcaagctta cattttgttt
     1861 gacatgctgt acaatttgta catttatctt tatcaatttt aaatctatat attgaaatag
     1921 gattaaaaaa accatagatt gctcctaaag ggcatatata cttgcaaaat ggcctataag
     1981 taatgattga catcaataat atcaatagca aaatacttaa cttccagcta aataaaaatc
     2041 ctattgattc tcttaaattc tcatttccta aaataagtgg tattcctccc aaaagtgttc
     2101 ctgaagggca aatccattta caaaaccaag gatttccacc ccctgctaca tttacaatag
     2161 caactggcaa aataattaca aacattatta agactatata ctttagatat tttagatatt
     2221 tatcaccata aacttttttt atctttactg gaaagggtac tttatgtagt aaatcttgta
     2281 caagtccaaa gggacaaagc caaccacaaa caaatctgcc aaaaaaagca ccaaatgcca
     2341 ttaaaaaacc aacaacataa aatgagaatt tgtactctct gcttgataaa actgcttgta
     2401 atgaacctat tggacatgct ccaagtgcac ctggacatga ataacaattc aatccaggta
     2461 cacataattt tttactttct cctccatata tagttccttc taaaaatcca tttacatatc
     2521 catttgttaa tgctgtaaat gcaacttgag taataagtct tatattatta tttaccttag
     2581 ataataactt catccctctt ctcctaacca ataccaatac actcaagaca aatcataatt
     2641 gctttagtta atactgtatt tgtttctcca cgatatgtac ctaatgcaat aaatataata
     2701 ctaattgcaa taatagaaat gcctttttta ttatctttaa taaagttact cattggcttt
     2761 taccattttt agtctttcat ctataatttt tttccaatct tccttactat ttgcacctac
     2821 aaccttctca cccacaaagt ttcctttctt gtctacaaaa taagttgttg gaaactccat
     2881 aactctttta aaatcagttt ttttaagttc ttcagataca gtcaattgtt ggtaagttgc
     2941 atctgacttt ttcataatgt ctttaactaa atttttctct tcatttgaaa gaccagttct
     3001 catatcagtt ttatcaactt ctacaactaa tccctttata gctacattgg aattattact
     3061 ctcatattct tttgataatg cttcaagctc tggcatttca cccacacatg gtccacacca
     3121 agtagtccag acattaataa gagtaagttc tttatttgaa aatatctctt ttgttacctt
     3181 atttccatca acatcctccg tatctaaatt actaaaccat tcactattat tcttgttttt
     3241 ttctactggc ttagattctt ttttgctaca tccagaaatc acacctaaaa caaaaataca
     3301 tcctaaaact actatgaaaa accttttcat atctcacact cctagaattt atatagtata
     3361 taaaatcgct gcttgaaata aaaataactt atcttctata ttaaagtaat tatatttaat
     3421 aaataagagc taatttaaaa aaagatatac agttaaa
//
LOCUS       DAMJYC010000201         3349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJYC010000201 DAMJYC010000000
VERSION     DAMJYC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.208"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     misc_binding    150..361
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            524..1813
                     /locus_tag="PC472_003944"
     CDS             524..1813
                     /locus_tag="PC472_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2729212.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1867..2946
                     /locus_tag="PC472_003945"
     CDS             1867..2946
                     /locus_tag="PC472_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2729213.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2967..3332
                     /locus_tag="PC472_003946"
                     /pseudo
     CDS             2967..3332
                     /locus_tag="PC472_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT         1214 a          362 c          668 g         1105 t
ORIGIN      
        1 gaaatttaaa aaaatacatt atataaatat agtaatagta tgaaaattta catataaaac
       61 taaaaaatgc aagtaattga ctttttaagt tgacaaaata ttgcatatat attatgatat
      121 aaatcataaa ttaactacaa atcaatatga tatattcgta gaagaagaaa gtagcataaa
      181 ggaattttac agagagttag atttggctga gaactaatat tggaatttat gtgaaaagga
      241 ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct taagaaaatg
      301 agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat tcgtctcttg
      361 gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa aactatatat
      421 taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa attgtaaaat
      481 atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag aagaaaaaat
      541 taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag ctatgttttc
      601 agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg agttggcaca
      661 gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt tttctgcaat
      721 tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat ctatgataac
      781 aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag gggtttctct
      841 attaggttgg tttggagttc aagtaggttt ctttgctcaa aatgcacaga taataataaa
      901 agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg gtctcttgat
      961 gatgagtact gcaatttatg gatataaggc aatggagaag ttgagtgtgt attcagtacc
     1021 atttttatta gttcttatga tgctaactat attcttagct tttagagcaa atggaatttc
     1081 agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc tgtcaatgag
     1141 tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta aaagtagaag
     1201 agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta tgataatagt
     1261 ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt ttattacatt
     1321 aggaattgct ataccaggta taatagtact tacattagca caatggacta caaatacaag
     1381 taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc ctgagaaagt
     1441 attaactata gtccttggta ttatagcaac attattggct gtatttggaa tatatgaagg
     1501 ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg gtgtatatac
     1561 agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag tattatataa
     1621 accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa cttacctttc
     1681 aacatatgga tttataactc ttactactat agcaccttta gatggtttta tagcagggtt
     1741 tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc aagaaataga
     1801 caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat atgaggagaa
     1861 agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg tgcagcagtg
     1921 cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa acaagccatt
     1981 aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga tgctttggta
     2041 gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact ttcagaagta
     2101 gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt ggatgttata
     2161 attcctatag aaataggagg gataaactca cttatgccta tagcagttgc tgctaaaaaa
     2221 ggacttccta tcttagatgc agattctatg ggtagagcgt tccctgaagc tcaaatggta
     2281 actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga aaaaggtaat
     2341 tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag aactttaact
     2401 gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc tcaagttaaa
     2461 aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt tttacttgaa
     2521 aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgcg tggatacaag
     2581 ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt tactagagga
     2641 catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt attccaaaat
     2701 gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga tttaatagca
     2761 gtattagatt tagaaactgg atttccaata actacagaga gaataaaata tggttctcgt
     2821 gttatggtag tagcttttcc ttgtaatgaa aagtggagaa cagaaaaagg aattgagaca
     2881 gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga attacaagga
     2941 aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt ggaggaacaa
     3001 atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt aaggtggcaa
     3061 ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata gatgaaagta
     3121 agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt acaaatgcta
     3181 ttgtagaaag aaaaagactt aataatgtgg ctatgataag aataggaaag ccagccacta
     3241 ctgccattct tccatttata gattggccag aagacttaag agaaaaaata gaattagatt
     3301 caatattggt atctggtgga tatgagtttg attaaaaaat agctacgca
//
LOCUS       DAMJYC010000202         3197 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJYC010000202 DAMJYC010000000
VERSION     DAMJYC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.211"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            2..613
                     /locus_tag="PC472_003947"
     CDS             2..613
                     /locus_tag="PC472_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2729214.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            911..1249
                     /locus_tag="PC472_003948"
     CDS             911..1249
                     /locus_tag="PC472_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2729215.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1656..3092)
                     /gene="miaB"
                     /locus_tag="PC472_003949"
     CDS             complement(1656..3092)
                     /gene="miaB"
                     /locus_tag="PC472_003949"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2729216.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1098 a          471 c          365 g         1263 t
ORIGIN      
        1 aatgataaac gatattaaaa ataaattcaa aaattacaaa ccatatataa atggatacaa
       61 gaatatgaag agagcatctg ttttaattcc aatagtagaa ataaataaca cacactatat
      121 attatttgaa gttcgctcaa aaaatttgaa gcatcaacct agcgaaattt cttttccagg
      181 tggtaaaata gaatcaggtg agactccata tgaagctgtt attagagaaa cttgtgagga
      241 acttggaact tttagtgaca atatagaagt aatatcaagt cttgacctcc ttataacacc
      301 agtaaagttt atcatacatc catacttagg atatataaaa aatattaata atttaaatat
      361 aaacaaggat gaagttgacc atactttttt ggttcctata aaatatcttt tagagaatcc
      421 tccaaatact tatagtaata ctgtaaaagt ttttccagat gaaaatctac catatggttc
      481 aattccaaac caaaaagatt ataaatttga aagtggaagt tatcctattc tgttttataa
      541 atataaagat tatgtaattt ggggaatgac agcaaaaata ctagaaaact ttttaaattt
      601 tttatcagaa taacactcat aataaattat caaaattttt acataagaaa tttataaaag
      661 tttctaaatt taacaattct aaaatatcaa taactatatt ttgtagcact tttttatttt
      721 attattgttt gaatatttga tggttttcaa tgcaatttca acataaatac tatatattag
      781 tgtatactgt tgatgaatta aagtatgata atctatttaa taaagtatga agtaaatgat
      841 tttaatgtgc ctttatggta aaattactaa taagtaataa aattaattaa aatatatgga
      901 ggtccttata atgtttgctg taggatttgt tgttataatt gcacttgcct ttttcatttt
      961 aagtgtacta ttatttaatg gaaaagccag tatgctacta gcaggttata actctatgag
     1021 taagaaagaa aaagcacgat acgataaaaa aaaattatgt agaagttcta gcattgttac
     1081 ttttattgtc tctataatgc tatttattat ggcgtttttg ggatatagaa tagagagtgg
     1141 tcaaatggac gaaaaaaaaa tggttccctt ttctataata tttataatag tcatactttc
     1201 cagtgtattt tttaatatcc tctactctaa caaaaaatgt aaaaaataaa tagttgtaag
     1261 ttgagcagtt aattataaaa gattgactgc tttttcatgt atattgacta ctcttattaa
     1321 atattttaac atttaaaaat ctatgtaaaa ataatgaata tagtacaaac ttctaataat
     1381 gattttacct ttgtggttac agtccactat atataattta atatgataac agggaattat
     1441 taatgacaac taagtaaact tttataaaat ataataaaac aaaaatagat gtacaatgaa
     1501 attatatctt atatcaaatt tatttttatt aatagcaaaa acttacatta gcataacata
     1561 tgacaaattc gcttaaaata aaaattttta atataatttg ttctatagta catctatttt
     1621 gattagttta cataatttta ttattttgta caatattaat tatttactaa tatcccattt
     1681 aatgaaaaag ttctaggttc agtaattttg accataacta atttaccaat taaatcatca
     1741 tttccacctt cgaaatttac gagtttattt tgtctagttc taccagcaaa cttattttca
     1801 ttatttttac ttcttccttc aactagaact tcaactattc tatctttata tccatcattt
     1861 atttctgcag aaatcctatt tacagcttct aacaccctat taaatctatc atgctttata
     1921 tcttctggta tttgattttc cattttagca gcaggagttc cctgtctttt agaataaata
     1981 aatgtaaaag ctgaatcata tctaactttt tcaaccacat ctaaagtgtc caataaatct
     2041 tcttctgttt ctcctgggaa accaatcatt aagtctgtag aaaaagcaat acctggaact
     2101 tcctttttag ctttttctat tatttctaaa taatattcct ttgtataatg tctattcatt
     2161 tttttcaaaa ggctactgct tccgcattgt attggtagat gtaaaaactc acacacctta
     2221 tcacaatctc taattgcgta tattacttca tcagatatat cttttggatg agatgtcata
     2281 aatcgaaccc tttcaattcc ttctatatca ttcacttttc ttaaaagttc tgagaatgaa
     2341 actggatttt caagtgtctt accataagaa tctacatttt gtccaagtaa agtgatttct
     2401 ttcgttccat ttgcaacaag ctcttttatc tcgttaatta tatcttcagg agttctactt
     2461 ctttctcttc ctcttgtgta tgggactata caatatgtgc agaaattatt gcatccatac
     2521 attatattta caaatgcttt taattcaaat tttctattac ttctaagacc ttctattact
     2581 tctccatcca catcccatac atccactaac attttgtcag aatttattga ttctgtaagt
     2641 aattgcggaa atttatatag attatgtgtt ccaaatataa ggtctacatg tttatatttt
     2701 tttcttaatt cctctacaac atgtggttgt tgcatcatac atccacatac agctattttc
     2761 atatctggat tttttccttt tagatgtttt aattgtccta agttaccata tacttttagc
     2821 tctgcatttt ctctaactgc acaggtgtta tatattatta aatcactttc ttcaaccatc
     2881 attgacattt ggtatcccat ctcttctaac attgaacata atttttctga atcatgttca
     2941 ttcatttgac acccaaaggt ttgagtaaac acgagttttc tctttccagt tctgttgaag
     3001 tattcttcat tttgtctgtg tatttgttct atatatttat cttgttcttg tattttctct
     3061 ataggtattg ttacttgttt tcttttgctc acgtctattc ctcctagtta atttcttata
     3121 ttacacataa ccttaattat atcaaataag aaaaatcttt taaagatatt attatttttc
     3181 tagttgtgtt ttaatag
//
LOCUS       DAMJYC010000203         3041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJYC010000203 DAMJYC010000000
VERSION     DAMJYC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.212"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            154..948
                     /locus_tag="PC472_003950"
     CDS             154..948
                     /locus_tag="PC472_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729217.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            949..3009
                     /locus_tag="PC472_003951"
     CDS             949..3009
                     /locus_tag="PC472_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2729218.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1165 a          314 c          500 g         1062 t
ORIGIN      
        1 tattataaac gaataaaaaa taataatttg tcagataaac ttgtaaattt ttatgaataa
       61 tccattctaa aacaaaaaac tgtgtataaa aaaggtttat atgttatatt tatataaata
      121 taacatatga acgagaaagg agtggtttga taaatgattg aggtagataa attgtgtaag
      181 tcttttacaa gggttgtaaa agatgataag aataaaagtt caataagaaa gctaaagaaa
      241 gtaaaaacta aaaaagaaga attttttgct gtaaataatg tttcatttaa agttatggaa
      301 ggtgaaattg ttggaatatt aggtccaaat ggtgcaggaa agactacatt acttagaatg
      361 ttaggtggca tattaacacc aacatcagga agtataaata tttctggata tgattattca
      421 atagatagaa attcagcaaa aaaagaaata ggttatctct ctggaaatac aaaactatat
      481 ggaaggcttt ctccgagaga acttctaaca acttttgcaa gtctatatga gatgtcaaaa
      541 gaagatatag aagaatcaat tgaaaatatt gtaaaaataa tggatatgag tgaatttata
      601 gataatagaa ttgaaaatct atcaactggt cagactcaaa gaacatctat agctcgttgt
      661 ttaatacatt ctccaaaagt atatattttt gatgagccaa cactaggtct ggatgtttta
      721 agtagtgcat ctattataga ttttatgaag aatgaaaaac taaggggaaa aactgttctt
      781 tactcaactc attatatgga agaagcagaa actttatgtg acaagatttt tatgattcac
      841 aatgggaaaa taattgcatc aggaacacca cagagtttaa aagaggaatc tggagtaaat
      901 aatttgagag atgtattcat aaaacttgcc agaaaggagg acatttaaat gagaagtaag
      961 attgtaaaat acatatttaa aaaagaaatg ttagacatat tgagagataa aaagacttta
     1021 tttatgatga ttgttgtgcc acttctttta tatcctttac ttatgttatt aatgattcaa
     1081 gtaatgaata tgtctgcaag tagtatgaat agtaaggaca ttaatatagc ttttaataat
     1141 aaacctaata aggtgttagt atccatgatt gaagatgata atttagaaaa agatactaaa
     1201 aatacttcaa atgataaagg caaaataaga gtgttagatg tagataacta taaaaagtca
     1261 ttagaagaaa ataaaattga tgcttatata aagatggaag aaaaaaaatc atctataaat
     1321 tatcaaatat atataaattc ttctcaagaa aattcttcta atgcactaaa aagaattgaa
     1381 actgttttgg ataaatataa aagatttacc atagaaaaaa ctttatctga aaaaggatta
     1441 gatgtacaag ctactttaga accaataaca tatagcactg tagatgttgc aaagacggaa
     1501 gaagtggcag gatacttttt aggtcagata cttccattta tattaataat aggagtttta
     1561 ctaggttcaa tatatccagc aatagatgtt atggctggag aaaaggaaag aggtacatta
     1621 gaaacattat ttacactgcc aatatcaaac ttagaactgg ttatgggaaa atatatggca
     1681 gtttctctta gtgcaatagt aacagcaatt ttaaacattt tatctatggg actgactatg
     1741 gcatttattt tagttagtgg aggtattagt agccaatttg gctttggaaa ctttaattat
     1801 ggagacttag tcttaccaat tataactact ttaatttgca tatgtctttt ttcaatggta
     1861 gtttcagcaa taagtatgtg tgtatgttca ttagcaaaaa gttttaaaga tgctcaaaac
     1921 tacataacac ctcttatgtt aattattatg ataccatcat atgtttctat gataccaaat
     1981 atagtattag atagagtgac agctgtaata cctgtagtaa atatatcttt acttataaaa
     2041 agtgtattat cttttaaaag cgatttaaat ttaatagcct tagttttagc atcaaatgta
     2101 gcctttgtta tattggcaat catactactg tctaagatgt tcaattctga ggaaatactt
     2161 tttggtaata gtaagagttt ttctttctta gagaaaagaa gtaatataaa aaaagggtct
     2221 ataccaactg taagtgatgg tgttatttta tatgcagtag gattagttct attgatttat
     2281 gttggttctt tagttcaaat taaatttggt atggcaggta ttataatgac acaattcatg
     2341 ataatttctt taccattatt atttgcatac tatattaaag ctgattttaa agaggtattt
     2401 agcttgacag ttcctaaatt aaagcatgtg tttgggagca ttgttctttg gattggtgga
     2461 tacatactta ttatgattat aacacagcta attttattct tatttcctca aaatatggaa
     2521 gttgctgaaa gtttaaataa ggctcttttt atcaaagata gtgttttact gaatttacta
     2581 gcaatagctt tattaccagc tatatgtgaa gaaatgtttt ttaggggatt tatattttct
     2641 tcatttagta aatcaaaaga taaaaataag tctatcaaac tcgctataat atgtagtggg
     2701 gtactatttg gtattatgca tatggatttt attagaataa ttccaacatc aatattaggt
     2761 attatatttg catacagtgt gtataaatct ggttcaatat ttgtatcaat gttattgcat
     2821 tttttaaata atagtgtagc agttgtgtta aatcattata caactggaaa tataaccaac
     2881 ttatataaat ttgttgaagt tgatttttcg aacttaaatg tgcttaaatt tgccttaatt
     2941 cttctaataa gtcttatatt aattatgtta ggattaagag ttttagatag aaagtcttta
     3001 agaaattaag tataaagtta ttatatagat taaaatatta a
//
LOCUS       DAMJYC010000204         3039 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJYC010000204 DAMJYC010000000
VERSION     DAMJYC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.213"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            292..996
                     /gene="srtB"
                     /locus_tag="PC472_003952"
     CDS             292..996
                     /gene="srtB"
                     /locus_tag="PC472_003952"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2729219.1"
                     /translation="MRKFLRIFINVILVLIVLYSSQKIYYKFDDYKKADKTYSKVRIE
                     RQEKQEKLDSKNKDFKFWLKIDNTNIDYPVVQAKDNKYYLNKDFYNQESKSGSIFIDY
                     RNNPNSQNLILYGHNMRNDTMFNNLLKFKDEKFFNQKNKIRIIKDDKEYIYEAFSAYT
                     IEANYDYLIPDFPNNKAFEEYIREVKNKSLFKSNLSVSDKDNIITLSTCSYEFEDART
                     VVHAKLINNLEGNLTY"
     gene            1092..1601
                     /locus_tag="PC472_003953"
     CDS             1092..1601
                     /locus_tag="PC472_003953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HDF2729220.1"
                     /translation="MNLIFNKSIKVKELNEKQEVVFYVDSFFDHMTKNIGYGLLLVIN
                     NRVIIKDLGLACMHSVNFSKNILGEIMGVIKATNIAFVNSYKEITIIHNSLGIEKWIT
                     NEWSIKDQVVMEYKKIIKNRTKYMKINFKIINTNANAYRKERYGDGHLKLIYEVRSLA
                     KFASKTSRI"
     gene            2037..>3039
                     /locus_tag="PC472_003954"
     CDS             2037..>3039
                     /locus_tag="PC472_003954"
                     /inference="COORDINATES: protein motif:HMM:NF019941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HDF2729221.1"
                     /translation="MKVTGKIFSITFLLFLLCISPISAFANEKLYQGYFENIKISGDE
                     PSVLLRITDGKGKDGKDNVVYCYDKNSQYPSALESADKTYYKRIESYLDSTDELTDKY
                     GKDKKERIAAVLNAGYPNDALGCMKKYGVSENDAMYMTQILIWDITLGTDVDYVASGR
                     ITESMAKYANEILNISKVIKFEQGKLVLEGKLEFTKKEKDWRTEKLYTTGNRGEFWFK
                     DIPSGFEIIDWNTDKKIDGNLSVGQEFYIKSSSEPSKNTRFNLEYKYNTVKFYFYKCV
                     SKNTHNNKKEYQNLIRLEPINKNNQISLEIKINGNFNEVITGGSGDKTWTEDTATEGM
                     "
BASE COUNT         1325 a          249 c          475 g          990 t
ORIGIN      
        1 ttttgacaaa gaaaataatg ataaattaaa gacaagctat gataaaacag taaaaatata
       61 aaatttattt agataacaag aatagaaaaa ctatcgattt tacaaaaaaa cctatcaaaa
      121 ctacaaaaaa tattgaatgg gatcttgtga atttatacaa tgttataaaa atataaggta
      181 aagcgtttaa taagagtgta tatatctaat ttagtaggaa taaaaaaata aatttgattt
      241 taacaatcaa tttttatatt taacaaagta gttaatatat ggagtggtat tttgagaaag
      301 tttttaagga tttttataaa tgtaatttta gtattaatcg ttttatatag cagtcaaaaa
      361 atatattaca aatttgatga ttataaaaaa gcagataaaa cgtattcaaa agtacgcata
      421 gaacggcagg aaaagcaaga aaaactagat agtaaaaata aagattttaa gttttggtta
      481 aaaatagata acaccaatat agactatcct gttgttcaag caaaagataa taagtattac
      541 cttaataagg atttctataa tcaagaaagc aagtctggat ctatatttat tgattataga
      601 aataatccta attcacaaaa tttgatatta tatggacata atatgagaaa tgatactatg
      661 ttcaataatt tattaaaatt taaggatgaa aaatttttca atcaaaaaaa taaaatacga
      721 attataaagg atgataaaga atacatatat gaagcatttt cagcgtatac aatagaagct
      781 aattatgatt acttaatacc agactttcct aataataaag catttgaaga atatataaga
      841 gaggtaaaaa ataagtcttt atttaaatca aatttaagtg taagtgataa ggataatata
      901 ataacactat ccacttgtag ttatgaattt gaagatgctc gaactgttgt acatgcaaaa
      961 ctcataaaca atttagaagg gaatttaacg tattaaacaa gtagaagtta attaaatatt
     1021 tagcatatat aagtttttga gttttatatt aaatttaata agagtttgat gttaatagga
     1081 gaaaaataaa tatgaattta atatttaata aaagtataaa agttaaagaa ttaaatgaaa
     1141 aacaagaggt agtattttat gtagatagtt tttttgacca tatgactaaa aacatagggt
     1201 atggtttact attagttata aataatagag tcataattaa agacttagga ttggcttgta
     1261 tgcatagtgt gaatttttca aagaatattt taggggagat aatgggagtt ataaaagcaa
     1321 ccaatatagc atttgttaat agttataaag aaataacaat aattcataat agtttaggaa
     1381 ttgaaaagtg gattacaaat gaatggagta ttaaagatca agtagttatg gaatataaaa
     1441 aaattataaa aaatagaact aagtatatga aaattaactt taaaattatt aatactaatg
     1501 ctaatgccta tagaaaagaa aggtatgggg acgggcattt aaaattgatt tatgaggtta
     1561 ggagtttagc taagtttgcc tctaaaacaa gcagaatata gttataagaa atgtaattat
     1621 atatcaacat ttaattaaca aatgttttat aataaactat ttaggatttt accctatagt
     1681 ttgcttatat ataaataaat tcatgcgtaa aaagataagt ttttagatga atagcgtgtt
     1741 tcaaatattg tttgaaatac gctatttttt atagatacat taatatttta tagaattatt
     1801 agtcagtaga ttattaagtt taatagcttc taattattac tatagaattt aaaattgctt
     1861 catatatgaa gtaaatatat aagaattatt tatcaatttt acataaaaaa taatcgaaac
     1921 tacaaattgc attgaaactt aatactaaaa agtttaattt attaaatagc ttttatattt
     1981 atatttaaat ataaagttac aatagatttc ttttagaaaa tttaaggagg gcaagattga
     2041 aagttacagg taaaattttt agtattactt ttttattatt tctactatgt atcagtccaa
     2101 taagtgcatt tgcaaatgaa aaattatatc aagggtattt tgaaaatata aaaatatcag
     2161 gtgatgaacc atcagtttta ctaagaataa cagatggaaa aggaaaagat ggaaaggata
     2221 atgttgtata ctgttatgat aaaaatagtc aatatccaag tgccttagag agtgcagata
     2281 agacatatta caaaagaatt gaaagttatc ttgactctac tgatgaactt acagataagt
     2341 atggaaaaga taagaaagag agaatagcag cagtattaaa tgctggttat ccaaatgatg
     2401 ctttgggatg tatgaaaaag tatggagtta gcgaaaatga tgctatgtat atgacacaaa
     2461 ttctgatttg ggatataacg cttggcacag atgttgatta cgttgctagt ggtagaataa
     2521 cagaaagtat ggcgaaatat gctaatgaga tactaaatat atctaaagta attaaatttg
     2581 agcaaggaaa attagtatta gaaggaaagt tagagtttac gaaaaaagag aaagattggc
     2641 gtacagaaaa attgtatact actggaaata gaggagaatt ttggtttaaa gatattccta
     2701 gtggatttga aattattgat tggaatacag ataaaaaaat agatggaaac ttatcagtag
     2761 gacaagaatt ctatattaaa tctagtagtg aaccttcaaa aaatacaagg tttaacttgg
     2821 aatataaata taacacagta aagttttatt tttataaatg tgtatcaaag aatacacata
     2881 acaataaaaa agaatatcaa aatttaattc gacttgagcc aataaataaa aacaaccaga
     2941 tttcattaga gataaaaatt aatggaaatt ttaatgaagt tataactggt ggttctggag
     3001 ataaaacatg gacagaagat acagcaacag aaggaatga
//
LOCUS       DAMJYC010000205         3009 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJYC010000205 DAMJYC010000000
VERSION     DAMJYC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.214"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(29..637)
                     /locus_tag="PC472_003955"
     CDS             complement(29..637)
                     /locus_tag="PC472_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2729222.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(746..>3009)
                     /locus_tag="PC472_003956"
     CDS             complement(746..>3009)
                     /locus_tag="PC472_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2729223.1"
                     /translation="YLLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDR
                     LIGVIYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMI
                     IQRTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKIL
                     TFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEI
                     KIDEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVD
                     MTLEQYFETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVE
                     EVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTS
                     RVLYTNRTFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIK
                     EVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHEL
                     KTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVK
                     SDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIIL
                     NILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGI
                     SNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDN
                     IEKFEIEFFDIYK"
BASE COUNT          953 a          490 c          241 g         1325 t
ORIGIN      
        1 tagatacata tcttacctat ataaaaactt attctatata aaaacgccat aaaaaatctt
       61 ttgcttcttc tgcataatct atatttatac gtttactagt ttttatatct aactcatctt
      121 tactaaatac tttttttcct ttatcatcat aataaaaatc acttatatat aattcttcac
      181 ccattatact cttactattt agacttctat ctatttttaa agctttgcaa agttttcctg
      241 gtccgttagt aatatttttt acttgatatt tactcaactc agtgtaagct ttgttgtatc
      301 tattcataga tatctcatct aaagaagtta tcggctctac ccctcttatt aatacacact
      361 ctggcacatt ttctatattt gcagataaat tcaagcaatt atacatacca taaattaaat
      421 aaacatatat atgtccacca tctaaatata atggcttagt tctatctgtc tttttattgc
      481 catatacatg tgctccttta tcatttacac ccatgtatgc ttctgtctct atgattttgg
      541 taacaattac tttattttca tatttcctta ttagatattt acctaatatt gatttggcta
      601 aatcaattcc attttttcta aaaaaatcct tttccaaaat ttcacctcat aaaatttatt
      661 taaaacgcat caaatacaac taaagtaata aataaaatta actcttatta atgtgatata
      721 tataaacaat aaatttagac atttattatt tatatatatc aaaaaattct atctcaaact
      781 tttcaatatt atcattgtaa tttgaattat catatacttc ttcaacaacc tctttaattg
      841 ggaatataac ttttacatta gttccttctc ctaccttact atctatgtaa atcttcccat
      901 tttgaatttc aatcaatgat tttgtaaggc ataaacctat accacttcca aattcatttg
      961 atataccact gttttcaatt tgtttaaatc gattaaatat atcatttaat ttttctttgt
     1021 ctatgcccac cccagtatct tttataatta tctctactgt ttcgtctttt ttatatatac
     1081 ctacaaggat ttctcctcct tctaaagtaa attttatagc atttgataat atatttagta
     1141 ttattctttc tatcgattct gggtcaagcc ctaccataac agtttcttca tctgtatcaa
     1201 aaattaaatc tattccttta gacttagcat aactactaat agatgtaact attctttctg
     1261 ttatgtcaat aatatcaaaa ttaactgctt taatcttata tatgtctgac ttaacttgag
     1321 aaatatttat tatattatct attaatctca ataatctaaa catattttgt ttagttattt
     1381 ttatatgttc ttttagtata tcctgaaaat caattatttt tcctttttta taaaggtttt
     1441 caataagttg aactgaacta tatatattat ttaaaggtgt cttaagttca tgagatatat
     1501 tagcataaaa ctccatcttc atttggtcat actgtctttg ttttattaat gcttttttaa
     1561 gtctttcaga ctctctttgc acacttaaat ctttaataat tctcactcta cttcttttct
     1621 gtccaaagta taatgttctt gatatcattt ttacttcttt aatgtgatta ttttcattta
     1681 ctatatggat actcttctct ctttctccta tatttaaatt gtttatgtat ttttttacta
     1741 atatatttct atatttttgt gttgtaactc ctaatacatc actagaaatt ccgaacaact
     1801 ctctaaaggt tctatttgta tatagtactc ttgatgtact taaatcctca agaaaaatag
     1861 cctctggtat aacatcaagt aatttttcat atattatttt ttgttcattt actgttctaa
     1921 gaatatttat ctctttagtt atatctctaa atgtaagcaa tttaaagagt ttattatctg
     1981 accaatagtc tccaactcta acttcttcaa catgttcttc accttctgag tcagacatca
     2041 caaaattaac tatacagttt gctgtgtcat tgtaacttac tatttcagct ttcttttctt
     2101 gtgttagatt taatctttta aattccctat tgtcaaagaa atctatgtta aaagtttcaa
     2161 aatattgttc taatgtcata tcgactaaat cacttgaaac acatttaaaa ctcttttcat
     2221 attttttatt tatacttatt accttattct tctttatgcc accctcagca cttcttacaa
     2281 gtataggaga tggaatattt agtataatgt tttcatagtc tttatttata tttcttattt
     2341 cttcatctat tttgatttca tttgtaatat catttaaact tattaagtaa ttgctttcat
     2401 ctttattatt acttaaactt aacaaataaa ttttatcatt taactctacc agtttatcaa
     2461 ttatacttat atcgttatca aatttcttca atttttcagc aactttttca tcttccttta
     2521 taaaagttaa tattttataa taattattca tatcctttga aaattcatct ttaaatttat
     2581 tatttgtgta acttatatta aaatcatagt ctgttactat ccatacattt ggtattgtat
     2641 ccagagcttg tctaagtaat tcttttctct ggtctatgta gttttttaat gttctttgta
     2701 taatcatatc cttttctatt gctaaattta tctttcctaa ttgctctgac tttttactta
     2761 attttttatt gattgaatca agttcataat atggatacac tactctatta ttaagactgt
     2821 atacatatac ataataagta tggttacata atagtaaata tattactcct attaatctat
     2881 ctgaatctac tatattaatc ttagaaattg ctacaaaaca tataatagtt gtaaaaaaaa
     2941 caaaatatgt taaatctttt attaacttat agtgctcaat gttactatca agtttattca
     3001 acaaatata
//
LOCUS       DAMJYC010000206         2958 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJYC010000206 DAMJYC010000000
VERSION     DAMJYC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.215"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(14..961)
                     /locus_tag="PC472_003957"
     CDS             complement(14..961)
                     /locus_tag="PC472_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729224.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(989..1480)
                     /locus_tag="PC472_003958"
     CDS             complement(989..1480)
                     /locus_tag="PC472_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729225.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(1923..2816)
                     /locus_tag="PC472_003959"
     CDS             complement(1923..2816)
                     /locus_tag="PC472_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2729226.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT          987 a          418 c          263 g         1290 t
ORIGIN      
        1 agctttaaaa tacttaataa tacaataaaa gtgatgtatt tacaaataat tcttctggat
       61 ttaattcatt tattgatgta tgttcaattt ttatatttat atttttcata tcttcataaa
      121 taattccaac tggagaacaa atgaaacaag aaaatccaaa agtttttgtt atttttttta
      181 ccttattatc accaaaactt atttgctttt tacaatcgtt tatattttca ttgttatttt
      241 gtatatcatt tttataatac ttatcttttt taatgtcatt agacttattt actgttttaa
      301 aattactcct atttttacaa ctatagtcta tttcaacttc tatataccca aaaactatca
      361 agttacataa tttcaaatta ctacctttgt ttataagatt tttcataggt ttaacatcaa
      421 atattttact atttacattt acttttaata tatctcttag atttttagga gcttttaaag
      481 tattacttat attcaattgt ttgaatacaa ttttatttac attagtataa actctattac
      541 tttctattga tattcttgtt gggaatttat ctagattaca cttataataa tagtatatat
      601 tagcactatt ctttataatc ctatgtgcag aatttggtaa aactacactc ttaaaagata
      661 ttattttttt acaacttgca tctattctcc ctatgtaaaa accttgctgt gggtctaatc
      721 cacgttttat aatgccatta acttttaccg tattatttat aaactctata tatttagaaa
      781 tataatcttt gaaaaatata tttccagttt tgtacctact tgtatttgta acaataatag
      841 tgtaggtcaa aatctcaaat acagatgcac attttttatc aacatatttt tctactttta
      901 taatacttcc atttgcatca tttgaaaata catcattaaa agtagttttc gttattctca
      961 ttgcatccac ctttctataa tttaaagatt actctatctt ggaactattt tcataactta
     1021 taattgcagt aacactaata aataaattat catctaaaat agatgaagta atatcttgta
     1081 taagatactt taaattaatt ttatctttat tttctatctt tctaggcatt tgtataaatg
     1141 tactaaaggg tatttttaat ttaaatgtat tttttttact agaatttcta tttcttttat
     1201 tgcctgcata atctaatatt gcatcaatat ctatatcacc aactaaaatc aacttctttc
     1261 ctgtaagttg ttcatcttct aatgatatac attctatagt atcaacaaga cttatattgt
     1321 taatattcat attgtaaaca tatgtctcta atgaatttat tacagttttt ttagaagcta
     1381 atttaaaatt tttatatgta ttaaattgag ataaataaag agtcttttct agtttttcac
     1441 atctttcaag gttgtatatt accttattat tttcatacat aatcgcatca atctccctgt
     1501 atctatatat taatactatt cataaaaatt atttcaaatt accatttttt attattcact
     1561 atctagttgt tatttacttt taagaaagaa tcctttttta cttcattgat ttcaatttac
     1621 aagtaaaagt ctttctgaaa aaaaaatata tatatctcta ttcacttttt tataagtaat
     1681 tataattttt atagactaac ggtcagcctt aagggaaaag gtgttcttta agtgcagaat
     1741 ttagataaag taaataatcc ttttataaat aattccaagt tttaacttaa ataatttgat
     1801 ttacagtata aagtaaattt tgaaagataa tgatatcgat ttaataatta caagtaaaag
     1861 catgtcaaaa taaggctatg attttaaaaa tcatagcctt atcaataaaa atttaatcaa
     1921 agttacattt cactttgaat ttcactgtct attaattgaa gattatactg ttgaattaat
     1981 tctattagac gagctgcata tatactattg ccattttcat ccttcgcagt actataacct
     2041 gccttttcta atgctttagc ttgatatata tacgtttttg catcaaatac accattgttt
     2101 ttataccttt tatttgtggc taaaaatttt gcatggtctt ttatagattc atttttatca
     2161 tcatatattc taaatttatc atctataatt gtatctaaat gttctttcgt ttctaaagtc
     2221 actgacttgc ctttccaata agcgtcagcc tttataccaa atagattgtt atattgtttt
     2281 gccaaagttg acttgcccca gctagattct attatagctt gtgcaatagt tatagatgga
     2341 agtattttat aatccttata attttgtatt gcttctgttt taatttcatc aataaacttc
     2401 atatattttg tatcagactt caatctttct ggttttatac cataatcctt aagattgtct
     2461 atataattat caaccaatct tttttgtcta ttactaaatt ctaactcatc caaaatatca
     2521 cttaacttat ttattttatt atttttagaa aaattcttaa caaataaatc tgatacttct
     2581 tgtatctgtg aatccttaac gttttttaag ttatttttat ttaacacagc aacaatggaa
     2641 gctacatact tccagttaac ttgtgcatta tttttacttg ctaaatcagc atattttata
     2701 tacttactga catttatatc atcctttttt atctctctag atgaatattc cttcactaat
     2761 acacctatta aaattgatat tatcagtatt ataacaatga attttttatt cttcataaat
     2821 attgtcctcc cttatactat ctgctgaata taatatacct tacttttgtg acatattctt
     2881 tacgaaattt tgaaaatttt atgaagattt atgaaatata tttaattaaa agcactacta
     2941 ttgttataat atatagtt
//
LOCUS       DAMJYC010000207         2891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJYC010000207 DAMJYC010000000
VERSION     DAMJYC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.216"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            269..2683
                     /locus_tag="PC472_003960"
                     /pseudo
     CDS             269..2683
                     /locus_tag="PC472_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1277 a          413 c          513 g          688 t
ORIGIN      
        1 atacaaaacc ctatttttta taacaattca ttaacataaa aatcaaactt accttaaaat
       61 ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca taatgactgc
      121 aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa
      181 attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata
      241 tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct
      301 tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc
      361 aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg
      421 gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc
      481 aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa
      541 tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg
      601 aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga
      661 gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc
      721 tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt
      781 aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa
      841 tggaacagca gttgcagaca aatttataaa agatgctgga ataactcaat cttatgtagt
      901 aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact
      961 aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac
     1021 agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga
     1081 tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa
     1141 gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata agataactaa
     1201 agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa
     1261 aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa
     1321 tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga
     1381 taaagcagta acagtaaact taaatggtac atatacaaag actgtaacta tcaacatgcc
     1441 taatggagat gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga
     1501 tggaactttt gttaactatg gaaaaataac taacttaaag gttaatgata aaaatggagc
     1561 caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag
     1621 tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaagggac
     1681 tactatagat aataaaggta caataagtag tgttaaggga gataattctc caacaataag
     1741 tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca
     1801 tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa ataatgaagc
     1861 agcaaaaata acaagcgtgc ctacaccagc aaaagatgca actagattaa ctatgccatc
     1921 agtatcaagt ggatatacta tagcaataaa aacatcaagt aatgagagtg ttataaaaaa
     1981 agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat ttacggtaac
     2041 acatacatca agtggtaaaa cagcagatac aaaagaatta tctgtaacag taccagctaa
     2101 atcaacagat gaagaattac aagcagcatt agacaatgaa gtggcaaaaa taacaagcgt
     2161 tcctgcacca gcaaaagatg caactaaatt aactatgcca tcagtatcaa gtggatatac
     2221 tatagcaata aaaacatcaa gcaataaggg tgttataaag gaagatggaa caataatacc
     2281 tccaaataca gaggaaacag taacattggt atttacagta acacaagaat caagtggtaa
     2341 aacagcagat acaggagaaa tagatgtagt agtaccagcc aaatcaacag atggggaaat
     2401 acaagcagaa gtacaagctg aggcggataa aataacaagt gtaactcagc caacacaaga
     2461 tgcaacaaca ctaactatgc caacagtagc aagtggatac acaataaaga taaaatcatc
     2521 aacaaatgaa agtgttatag cgaaagatgg aacaataata cctccaaata cagaaacaaa
     2581 agtaacatta gtatttacag tgacacacac atcaagtggt aaaacagcag atacaggaga
     2641 aatagaagta acagtaccag ccagaacagc tatagataca gaacaattgg cagtacaagc
     2701 tgaggcagat aaaataacaa gtgtaactca gccaacacaa gatgcaacaa cactaactat
     2761 gccaacagta gcaagtggat acacaataaa gataaaatca tcaacaaatg aaagtgttat
     2821 agtgaaagat ggaacaatag tgccaccaga tacagaaaca aaagtaacat tagtatttac
     2881 agtgacacac a
//
LOCUS       DAMJYC010000208         2790 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJYC010000208 DAMJYC010000000
VERSION     DAMJYC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.217"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(339..1046)
                     /locus_tag="PC472_003961"
     CDS             complement(339..1046)
                     /locus_tag="PC472_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2729227.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1850..2719)
                     /locus_tag="PC472_003962"
     CDS             complement(1850..2719)
                     /locus_tag="PC472_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2729228.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITSEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          967 a          458 c          245 g         1120 t
ORIGIN      
        1 tactataaaa tttttagtct tcatcaatac cttttttgct ttcattgtta aagctatttt
       61 ttctattagt ttatttctta taaaacaaac atttagtatt aaaatatata tcacacaata
      121 gtatttgcaa tttaacttta aaaaaataaa aatacccaac tgacctaagc aactcgattg
      181 ggtataaata atctaaaaaa tcatttttgt ataaactctt tatttaactt acatttttat
      241 ttaccttcta ctttaattca acttatattt ctatgcttct tatactctca ttcaacttat
      301 atttctactt atcttatact tttataaatt catcacaatt atttagttct cgctatcaaa
      361 gaaattgtat taaaatactt tccattctct acttccatca tttttttgtc atttatagca
      421 tatgaattat cacaatttat ccaatccttc caagcttcat catgacaatt taaagcttca
      481 catttctcta caatcacaga ctccgattca gaccaaagtt tattccacca tgtaattgaa
      541 tggaagttca tattatcttg ccaaaatgga atgagttcat ctggtataca atccacgaaa
      601 tcttctttta atccaggcat tgccatagca agtattcctc cctcttttac aagaggtgat
      661 atatgattgt ctaaaaatcc ttctttgttt ccaaagtagt gataagaatc gatacttatc
      721 accgcatcaa agaaaccttc tgcataaggt agttcatgtg cctctgcctg tattggaaat
      781 attttatcat ccaatttaaa ctctttaaat cttttatagt tttcagttgg ttctatccat
      841 aaatctgttg caaatacagt agcatcaaat tcctttgcta aaaatatcga agatataccc
      901 ttaccacaac ctaagtctaa aatacgcatt cccttttcta gttttatctt acttgttaat
      961 tcttccaata tttttataca attaggtccc atcatatttt tcatgagaaa gtccttatcg
     1021 tatttattac tttttgtaaa tatcattcta tctttccttt ccctatcagt tattatttat
     1081 taaattttat acgtttgatt ttttattttt tatatgcttc ttatattgag caatctcttt
     1141 tttattcatt ctttaacaga caattttaat gtgtaatttt atccactctg tgatagtaac
     1201 tttaatgaat aatcttatct actgtgatag ttaactttga tgggtaattt tatctactcc
     1261 acagcaatcg ctttaatgag taatcttatc cactctatga tagttatttt agtgagtaat
     1321 tttatctact ctacagtaat cgctttaatg agtaatctta tccactctat aatagtcact
     1381 ttaatgagta atcataattt ttatatcatt ttagatactt actctaataa gaagtcattt
     1441 tctaatactt aataagtttt aattacttcc actttaacct atatactaat taacttattt
     1501 aatttagtgc atatttgaca ctttaccttt tattatacca tttattatca taaatataat
     1561 aattctattt ttttaaaata aatatttata ataatctaaa ataaatattt ataaattcta
     1621 atcaatccta aataataatc taaaatacat caaaaaataa actaaaaaat atcgaaaaat
     1681 aatttaaaag acatcaaaac taaatcttct aatcagcaat taaaagtcta ttttcttagt
     1741 ttaaaaaata aagatagatt ttttcaaaac actggataaa taggtaaaaa aataaacagt
     1801 tagtaaaata aatttttact aactgtttat ttttattgtt taatatgtcc tataaatcct
     1861 ctaagtaata ttttccttgc aaaaattctg attctaaacc tagattttta aaataatcga
     1921 ccatgcccat acacgcaaat ttttctgtag aataatgagt agcacctata acattaatta
     1981 aattatcttt tgctatttga tgaaactcta atactggttc aaaatgtttt aaaggcttag
     2041 taaacccagt aatatataga tttataccaa gttcagcaac ttctcttgct acaaatggat
     2101 aacttcctcc ccctgctgca atagcaacta aaccatcttt taatacagaa tctccataat
     2161 catacaattt cacatcatga ccaacagatt ttttaattaa ttccttaatc tcttctattg
     2221 attttagtga tgttttacat ataactccta cctcaatttt atcgtcatat ttacaaaatg
     2281 gtttgactat ttctaaaccc atagcttttg caaaactaac agaagttgaa tagtcactat
     2341 agttgtctag tggagaatgt aatacataaa aagaaattct acgatttttc atctcttcca
     2401 tataagatag tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg tatcccattg
     2461 catgatgtga aaaaattaaa atatcttttt catctttatc tataacctgc ttaattattt
     2521 ctttatcagg aaaagtagtt gtgtatactt tattaatatt ttgagcatta tctaatacta
     2581 atcctatgta tttttttcta aattctgaag tgataaaatc attttcaaaa ttcataaaac
     2641 tccaatcatc attaatattt ttaatattaa attctaaatc taatttttta taaagttcaa
     2701 ttgccttaat aataaccact cctttataat aatattgaat tactacatat taatatagaa
     2761 atttcacgat aataaacttt atacgtttaa
//
LOCUS       DAMJYC010000209         2778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJYC010000209 DAMJYC010000000
VERSION     DAMJYC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.218"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            33..836
                     /locus_tag="PC472_003963"
     CDS             33..836
                     /locus_tag="PC472_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2729229.1"
                     /translation="MNKVLNWPGAKWSMSKNIVGILPKHNIYLEPYFGSGAVFFNKKA
                     CNTEILNDADKQIVNLFKCIRDNPNELMNAIYFTPYARDEYMNCNILETDNDIEKARK
                     FIIRTNMARGGIQNYKTGWKHTGPKESKKCFQKVVGKWNKLPKIILDTAIRLKDAEIE
                     NRDAVELIKKYNKTDCLIYADPPYLLKTRSQKMYNVEMETEEEHEKLLKTLLKFSGYV
                     ILSGYDSDLYNEMLNGWNKMEFKACAEQGKRKEMLWTNFETTKQLSLFK"
     gene            849..1256
                     /locus_tag="PC472_003964"
     CDS             849..1256
                     /locus_tag="PC472_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2729230.1"
                     /translation="MRINFTIDGEPVGKERPRFNLATKRTYTPNKTKSYEELIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            1358..2206
                     /locus_tag="PC472_003965"
     CDS             1358..2206
                     /locus_tag="PC472_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2729231.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKAYK
                     NKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            2309..>2778
                     /locus_tag="PC472_003966"
     CDS             2309..>2778
                     /locus_tag="PC472_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729232.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENEL"
BASE COUNT         1220 a          250 c          522 g          786 t
ORIGIN      
        1 gaatgtagaa agaaggctaa gaaggtgaaa aaatgaataa ggttttgaat tggccaggtg
       61 ctaaatggag catgtcaaaa aacatagtag gtatacttcc aaaacataat atatatttag
      121 aaccatattt tgggagtgga gcagtatttt ttaataagaa agcatgtaac acagagatat
      181 tgaatgatgc agataaacaa attgtaaatt tatttaaatg tataagagat aatccaaatg
      241 aattaatgaa tgctatatat tttactccgt atgctagaga tgaatatatg aattgtaata
      301 tccttgaaac tgataatgat atagaaaaag ctagaaaatt tataataaga acgaatatgg
      361 caagaggagg aatacaaaat tataaaacag gttggaaaca cacaggtcct aaagaaagta
      421 aaaaatgctt tcaaaaagtt gtagggaaat ggaataagtt acctaaaatt attttagata
      481 cagctataag gttaaaagat gcagaaatag aaaatagaga tgcagtagag ttaataaaaa
      541 aatacaacaa aactgattgt ttaatatatg cagaccctcc ttatttacta aaaacaagaa
      601 gtcagaaaat gtacaatgta gaaatggaaa cagaagaaga acatgaaaaa ttgctaaaaa
      661 cacttttaaa gttcagtgga tatgtaattt taagtggata tgattcagat ttgtataatg
      721 agatgcttaa tggatggaat aagatggaat ttaaggcatg cgcagaacaa ggcaaaagaa
      781 aagaaatgtt gtggaccaac tttgaaacta ctaaacaact tagtttgttt aaataatagg
      841 agagagtaat gagaattaat tttacaatag atggagaacc agttggcaaa gaaagaccta
      901 gatttaattt ggctactaaa aggacctata cacctaataa gactaagagt tatgaggagt
      961 taataaaatg gctgtatcaa tctaaagtta aacattactt tgaaggctat ataaaaatga
     1021 ctttaagatg ttattattct atagctaaaa gtaacagtaa aaaagttaaa gagcagaaaa
     1081 gaaataatgt attaagacct agtaagaaac ccgatattga taatgtagtc aagattatag
     1141 ctgattcact taatgagata gcttataaag atgatacaca gattgttgag gttgtagcta
     1201 gtaaatacta tagtgataag ccaagggttg aggttatatt agaagatgtt atctaaccaa
     1261 cggaaaaatc cgttcgttaa attatgccct tagtttttca taaaaatgtg aaaaagctaa
     1321 ataaagaata tatcaaacga caaaggagag ataaattatg aatgaaaata taaataaaga
     1381 aataacagta cttggaactt tagaaatcga gggaatgaaa tttcataaca ttgagggtgg
     1441 atttggagaa cataagaaag caatgctagt aaaagatata gccgagatac ataatagaga
     1501 atctagacag attaatgagc ttataaataa gaatagaaaa agatttaaag atggaaaaga
     1561 tatattagat ttgttaggtg tcggtttgga cgataccaaa ataaaagaat taggatttac
     1621 tcaacaatca attaattctt atagagggtt aaaaaacaaa gggttattat ctgggattta
     1681 tatattatct gaaagaggtt atgcaaaatt attaaaaata ttagaagatg atatagcttg
     1741 ggaattatat gagaagttag ttgatggata tttctctatg agaaaagaac taaataatcc
     1801 tcttttaagt gcatcaaagg agttacaggc tatatttatg ctagataaga aacaagaagt
     1861 cttagaaact aaaatagaga atgttaatga gaaattagag aactttatgg atgatgcacc
     1921 actattcaat attgaatgtg aaagtattgt aaaagaagtt aagaaggtag caacaaaatc
     1981 actaggaggt catggaagta aggcttataa aaataaatct ttaagaggta aagtatacaa
     2041 tgatatatac catcagatta aacgagagtt tggagtagat agttataagg ctataaagcg
     2101 ttgtcaatta aataaagtat tagagattgt aaacaattat aagttaccta tagtgtttga
     2161 agaagaaata agacttttaa atagtcaatt atcaatagta agttaaattt attcaaaaaa
     2221 aaggaaaagg agtgctttca cactccactt gtcaaaaata taaaactttt atccaagatt
     2281 attataacat aaacaggagt gtggaagtat ggataataat atcaataaaa aagaactatt
     2341 taaaaaagta gaaggtagat tacatcatta taaattttta agtgcagaaa ttaagaatct
     2401 tgaattagat atagaaagta gagaaaatga gatatttggg tgtaaggctg ttggatatga
     2461 tgaaaaagta agtccaacat atgcttttaa ttcaactgtt gagaatgaga ttataaaaaa
     2521 agaaagagat attactagat tgaaaaaact gaaaaaagat aaagaaatag aaaagaagaa
     2581 aatagaaaat gcacttacat gcttagatat aagagaagaa cattttttta aactgtttta
     2641 taatagtaga atgaaaaata gtatggttta tatatcctta gagatgaact cagataggaa
     2701 aacatgtaga tgtgtaaggg aaagattagt gtataaagtt atggatatgc tttatccaag
     2761 aattaaggaa aatgaact
//
LOCUS       DAMJYC010000210         2756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJYC010000210 DAMJYC010000000
VERSION     DAMJYC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.219"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            116..1048
                     /locus_tag="PC472_003967"
     CDS             116..1048
                     /locus_tag="PC472_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2729233.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1023..1850
                     /gene="panB"
                     /locus_tag="PC472_003968"
     CDS             1023..1850
                     /gene="panB"
                     /locus_tag="PC472_003968"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2729234.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1869..2717
                     /locus_tag="PC472_003969"
     CDS             1869..2717
                     /locus_tag="PC472_003969"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2729235.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1105 a          332 c          448 g          871 t
ORIGIN      
        1 tataagaact atactttaaa tgtttttaat tattttgtct tatgtaactc atatcctcag
       61 gaatgagatt aaaaattaag atgttgtatc acaaagtgga ggtgatacta ttaaaatagg
      121 ctttattggt tctggaaaag ttggaacatc tttggcaaat tattttttat caaaaggtta
      181 ttgtgtttca ggcttttatg gaaggaatca attatcttta ttagaatcaa caaatttaac
      241 aaaaacaaag atttacagca atttgaaaga tataatttat gaaaacgata tattatttat
      301 tacaacatca gatgattcta ttgaaattat agacaaaaaa ctttccaaat ttaatttaaa
      361 acataaatac atttgccaca caagtggctc tttaaaatct agtatattgt tctgttcaaa
      421 aaaagcaggt gcattaattt attctatcca tcctatattt gcattttcca ataaaaatac
      481 caacataaaa aaaatgaaag acatttgttt ttccatagaa ggtgataatg aacaagatga
      541 tttagttatt caaaatttca tagatgattt gggaaaccaa ttttttataa gagataagga
      601 tacttcatcc acttatcatt tagctaatgt tttagtttca aatttagtat tatctttact
      661 agatatagga gtaagttact ttattaaatt aggtttatct gaagaggaat ctttaaaagc
      721 tatcagtcct ttagtaaaaa aaaatataga aaatatactt gataatggct ttactaaagc
      781 tcttactggt cctgtttctc gtggagatat aacaccaata agaaaacatc tatctgtatt
      841 ggataaaaaa gatgaggata tttacaaaat actatcctta aacctactaa aaataattgc
      901 cttaggaaat gacaattcat taaaaggtat aaaaaatatt actatagaaa atgcaatcga
      961 aaatttaata agtaaatctg aaaaatacaa agaaatttac aaacttttag gaggaaaaaa
     1021 cgatgaaaaa taccatacag acttttaaga atgctaaata tgaaggaaaa aaactatcta
     1081 tgcttactgc ttatgactac tcaattgcaa aaataatgga tgagtgtgat ataaatggta
     1141 ttttaatagg agattcatta ggaatggtca taaaaggtga agaaaacact ctatctgtta
     1201 ccatagacga aattatatat catacaaaag ctgtaaaaaa tggtgtaaaa aatgccttaa
     1261 tcgtaagtga tatgcctttt ctatcttacc atgtttctat agaagatgca gttaaaaacg
     1321 ctggtagact tataaaagaa ggaggagctc atgctgtaaa gttagaaggt ggctcaaacg
     1381 ttataaagca aatagaaagc attgtaaatg ctcaaatacc tgtaatgggg catttaggat
     1441 taactcctca atcagtaaat tcatttggtg ggtttaaggt tcaaggaaat accagtgaaa
     1501 cagcaagaca acttattgaa gatgctaaat taatagaaaa agcaggtgca ttctctattg
     1561 tccttgaagg agtgcctacg aaaatagctg agatggtaac taattctatt tcaattccta
     1621 caataggtat aggggcagga attaactgtg atggacaaat tttagtttat caagatatgt
     1681 tgggaatgtt tggagatttt gttcctaagt ttgtaaagca gtatgcaaat ataggagata
     1741 taatgaaaga ttcaattaaa aattacatcc tagaagttaa tacaggtgca tttccacaag
     1801 aaaaacacag cttttcaata aatgaatctg agttagaaaa attatactaa tattaaggat
     1861 ggtacaaaat gcttgtaaaa gaaattaaat tactaagaaa tataatcaaa gattggagaa
     1921 aacatggcta ttctataggc cttgttacaa caatgggatt tttacatgaa ggacaccaaa
     1981 gtcttataaa aaaagctgta aaagaaaatg ataaagtagt agtgagtgta tttgtaaatc
     2041 caactcaatt tggaccaaat gaagatttta atagctatcc aagagatata gataaagatt
     2101 ttaaatattg tatggatagt ggagcaacag tagtctttaa cccatcacca gaagaaatgt
     2161 atttaaaagg taattgtact accattaatg tttctggact tacagatttt ctttgtggag
     2221 ctaaaagacc tgttcatttt ggaggtgtct gcctagttgt ttcaaagttt ctaaatatag
     2281 taacacctga taaagcttat tttggagaga aggatgccca acaattagct gtaattaaaa
     2341 gaatggtcaa ggatttaaac atagatacag aaataatagg ctgtcctata attcgtgaaa
     2401 atgatggatt agcaaaaagt tcgagaaata catatctctc tgaggaagaa cgaaaatctg
     2461 ctctaatatt aaataagagc ttaagtttag caaaagaaga attagtaaaa ggcaatctaa
     2521 atccagagaa tataaaagaa ttaataacag ctaaaataaa ttctgaacac ttagcaaaaa
     2581 tagactatgt tgaaattgta gatagtgaga cattacaacc tgttaagcaa atagaacatt
     2641 ctatacttgt ggcaattgct gtgtttatag gaaaaaccag actaattgat aattttacat
     2701 ttgagttaaa tatatagtct ataattttat ataatatatc taaaaaataa tagaat
//
LOCUS       DAMJYC010000211         2741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJYC010000211 DAMJYC010000000
VERSION     DAMJYC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.220"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            200..1798
                     /locus_tag="PC472_003970"
     CDS             200..1798
                     /locus_tag="PC472_003970"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2729236.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2033..>2741)
                     /locus_tag="PC472_003971"
     CDS             complement(2033..>2741)
                     /locus_tag="PC472_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2729237.1"
                     /translation="EIGPTGPTGVTGSIGPMGAIGPTGPTGVTGSIGPMGVTGPTGEI
                     GPTGATGPTGNTGATGSIGPTGVTGPTGATGSIGPTGPTGATGNSSQPVANFLVNAPS
                     PQTLNNGDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYS
                     FAILFPILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSA
                     GTAATLTIFRIADTVMT"
BASE COUNT          944 a          402 c          424 g          971 t
ORIGIN      
        1 atttaattcg atatttgatt gggtaaaagt atatataata ataattttta tattaaattt
       61 gtagtattta tgtaagaaat aaattttata taatattttt tcactaaatt tttttctttt
      121 tacaacaaat actcactacc ttttgatata atatttttat caattattat tatattcaag
      181 ttttgaaagg agtattaaaa tgagtttatt agttttcgga cataaaaatc cagatacaga
      241 ttcaatatgt tctgcaattt cattagcaca tttaaagaac aaactaggaa taaaagcaac
      301 agcttatgct ttaggagata tcagaaaaga agctaaatat gccttggatt actttaaggt
      361 agatgcacct gaaattttag ataatgttag aatacaagtt agagatttaa attatgataa
      421 agtagtacct ttaacaccaa cttcatctat attagaggct tataacctta tggatgaaaa
      481 aaatgttaaa acactacctg taacttatga tgatggaact tttgctggta tcattactat
      541 gaaagaaata gctaaaaatc tacttcatca acactttaca actatacaca catcactatc
      601 aaacatttgt aaaaacctta atggtactat tcttgtggac tgtggtgaag atataaaagg
      661 tagagtagtc actttatcat ttggaatgga gactctaata gagactttaa aagaaggaga
      721 catagctatt gttggagata gatatgatag tattgaatat gctattgata caaaagttaa
      781 acttttaata cttactaatc atacagagat ttcaaaagat ttgctagctc ttgctaagat
      841 aaatggagtg tctgttgttt ctgttgaaag tgacaattat aaaacttcta atgtaataaa
      901 tcaatgtagt ttcttagact ctatagaagc tacagaaaat ttaattacat tcaaagaaga
      961 tgattatatg gatgaaataa aagagattat gctagaaaca aacttcagat cttatccaat
     1021 attagatgat gataataagt ttttaggttt agtttcaaaa ggtcatctat taaacccatc
     1081 taaaaagaat gttgttttag ttgaccataa cgaatatgct caaagtgctg atggaattga
     1141 acaagcaaac atagttgaaa taattgacca tcataaactt ggtggtattt ctactgatgt
     1201 tcctatgtca tttagagtta tgcctgttgg atgcaatagt actataattt atcaaatgta
     1261 caaagaaaat aatgttgaga taccttatga aatagctggg cttcttttat ctgctatatt
     1321 atcagataca ttattattta agtctccaac aacaactgat atagataaga aagcttgtga
     1381 agaattaagt aaaatagcaa aagtagacat ggaaaaatac gctatggata tgtttaaatg
     1441 cggtacttct ttagatgaat atactataga agaaatagta aatatggact ttaaagaatt
     1501 taatatgagt ggacatagag ttggtattgg acaagtattt accttagaca tagactctat
     1561 atttgctaaa aaagatgagt ttttatctta tataaattcg actgattatg ataaattaat
     1621 tctttcagtt acagatataa tcaaagaagg ttcttattta atctataaag cagaagatag
     1681 cttaattgct aacgcatttg gtgttgaagg tgttcaaggc acatttgcac caggtgttgt
     1741 atcaagaaaa aaacaattag tccctaattt aactactgca atcaaaaatt ttaaataata
     1801 taaaattttt aaataacgaa aactgtctca aatctatatt tgagacagtt ttttattata
     1861 tatatattga taataaagtt aaataaaaat ccataaattt aaaattttta tgaatatatt
     1921 ttattattat tgttatttta ttcattctaa aataggtaaa actcaatata taaaaatact
     1981 tttgtcattt aaataaagcc taaatagttt ttaagattat tttagacacg ttttaagtca
     2041 taacagtatc agctattcta aatatcgtca aagtagccgc agttcctgct gattgcccat
     2101 ttcgtatccc tactgttgta gatgaaaaat tatttattgt aaaagtagtc ccaccaaata
     2161 aaaaaccagc aaaatatcca gaaagtactc ctccgcctcc tggctcagta gtaagccctg
     2221 ccaaatcttt tcctcctaaa attgggaata ggatagcgaa agaatattga tttatacttg
     2281 atgaacctgg ttgtaatgct actgaaactg atatataata cacaccattt tcttgtactg
     2341 taaacgtacc atttgcatct actgttatac ttgaactatt tcctattatt gtttgccaac
     2401 ctgttatagc atctccatta tttagtgttt gtggagatgg tgcatttacg aggaagttag
     2461 caactggctg agaggaatta cctgttgctc ctgttggtcc cgttggtcct atacttcctg
     2521 tcgctcctgt tggacctgtt actcccgttg gccctatact tcctgtcgct cctgtatttc
     2581 ctgttggtcc tgttgctccc gttggtccta tttctcctgt tgggcctgtt actcccattg
     2641 gtcctatact tcctgtcact cctgttgggc ctgttggacc tattgctccc attggtccta
     2701 tacttcctgt cactcctgtt gggcctgttg gtcctatttc t
//
LOCUS       DAMJYC010000212         2691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJYC010000212 DAMJYC010000000
VERSION     DAMJYC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.221"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..733)
                     /locus_tag="PC472_003972"
     CDS             complement(<1..733)
                     /locus_tag="PC472_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2729238.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     TVYTGKVDKSNNYLAEQCEIANRQNVDVAVQIHFNADHTTLNSMGTETIYKTNNGKIY
                     ADRVNTKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNEGVKQMYKHTIVYDGEVDKILANVLSWGYSPSKVLVCDIKDYV
                     PGQTENLYVVGGGACEKISSITKEKF"
     gene            complement(733..990)
                     /locus_tag="PC472_003973"
     CDS             complement(733..990)
                     /locus_tag="PC472_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2729239.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            complement(1010..1240)
                     /locus_tag="PC472_003974"
     CDS             complement(1010..1240)
                     /locus_tag="PC472_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2729240.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            complement(1281..1463)
                     /locus_tag="PC472_003975"
     CDS             complement(1281..1463)
                     /locus_tag="PC472_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729241.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            complement(1463..1756)
                     /locus_tag="PC472_003976"
     CDS             complement(1463..1756)
                     /locus_tag="PC472_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729242.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            complement(2143..2691)
                     /locus_tag="PC472_003977"
                     /pseudo
     CDS             complement(2143..2691)
                     /locus_tag="PC472_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          801 a          495 c          309 g         1086 t
ORIGIN      
        1 taaatttttc tttagtaata gaacttatct tttcacatgc gccacctcct acaacatata
       61 aattttccgt ctgacctggt acgtaatctt ttatatcaca aactaaaact ttgcttggac
      121 tatagcccca actaagcaca ttcgcaagta tcttatcaac ttctccatca taaacaattg
      181 tatgtttgta catctgttta actccctcat tatttatatt tttatttaaa ataccttctg
      241 ctattaactt agcaactatg tctttatgtc taatataata atctgtatct gctttgctat
      301 ctacgaagca cacttctatt aatatcgctg gagcttttgt atgactaagc cagtaaagcc
      361 ctctgacatc tgattttgca cctctgtttt taaatacagt tgctaattta gtatttaccc
      421 tatctgcgta tattttcccg ttattagttt tgtaaattgt ctctgtaccc atagaattta
      481 atgttgtatg gtcagcgtta aaatgtattt gtacagctac atctacattt tgcctattgg
      541 caatttcaca ttgttctgct aaatagttat tagacttatc tacttttcca gtatatacag
      601 tagctccacc ttgtttcaac cattttacta ttaaatcagt taggattctg ttttcttttc
      661 cttcatctat atacccagtt gctcctgttc cttttccact taaagtgtgt cctggtacta
      721 ttgctatttt cattatttat caccatcctt caactgtttg taaacttgat ttgcccctat
      781 tgcaactccc caacataaaa ttccttgtaa aattgatgaa ggattaaatc ctaacatcca
      841 tattgaaaat ccaattccta tcacaagcaa tatgattgga atgtatttat tatctaactg
      901 tttgtatttc ttaaatccaa atcctaatac attaagagca actactaata aaagcagttg
      961 ctctggtata aaacttatta aattatccat atcttaacct cctaattaat taaaatattc
     1021 ctttctgtat ggcaaatata aagaacccta ctaaagttgt aattataact cctacaagcc
     1081 acttgagtac tcctgtcaga ttttttatat cactacatag gttttgtatt tgtatagcaa
     1141 actttgcttg ctcaatctcg attctatcaa tttgtttgcc gtgttctttt actcttgttt
     1201 caagtgtact tattttttct ttcacaagtt cttcattcat gaaaacctcc tattgctttg
     1261 tattaaaaaa agaacattac ctattttgta agttctgctc cttctactac ctcactatgt
     1321 tctataatgt agttttcaac tgccatcctg tactctgtgt tagtaacatc atcaagttca
     1381 aatactctat tttttagagg atttaatccc ttatttaata ttctctctgc taatattctt
     1441 actacaacat tatttatatt cattataaaa ttcctcctac tttctcattt tcatttaata
     1501 aaatttgatt ttctaactct tgtattctct tttcttcatc tgaaataaat gttggtattt
     1561 cctctaaaat tggttctttt gtttctatat ttatacctat aattctattt cttgtatagt
     1621 ctatacttcc atacggaata tcaacaaaat gtaattccgt tatttcatca tgttctaata
     1681 tatcccctgt tgcttctcca gtttggagta atattttccc ggtttggtca catataattc
     1741 tatttcctct ttccatttaa attaccacct ttcatattat tcataagcat accatgtaaa
     1801 actttctccg actttacaac ggtatactac gctactacta tcagtgtatt catctcgata
     1861 agtatataaa gaaaattctt tgctagtaat agtttttaca aacataaaag cacaaaaacg
     1921 atattctcca ctactcttag gaacacgacc aacagaagca agttgattta aattattaat
     1981 agcataaaaa ttaacagtat acccatttcc ataaaaacaa agtttttcaa caatgacaag
     2041 actagggatg aaatttaaat taataggaac agtaagactc ttagcataat tttcagcact
     2101 tgaatacctt gaataaaaat tttcataatc actttgagta aaagtgtaag tacccttagc
     2161 ccatctttta cgttggctta actgattatt taaattagta atctgatttt gaagttgttc
     2221 agcagtagca tctgaactat caaaacctgt ttttattttc tctgataatt caactaatgt
     2281 attatttaaa tttgcttcta tattttttaa tgctaaagtg tttataatac ttgtttttcc
     2341 agttttaaat cctgcattaa cttcaattag tttagaagat atgtcttgca gattcacatc
     2401 ttcgggcaat ggcattatat tcttacttat acttaacact ttttctgctg tagcattatt
     2461 actgtctgta acaactatct taagtgtgtg tagtgcatta tcttctagtg tatagttaat
     2521 tgttttctct gttgttaaat ctgttgttat agtttctttt aacacatcat ctataaaata
     2581 ttctattttt gtaagcaatg tagggtctgt gtggtcagct ttaaatgtag ctgtaatgga
     2641 attataagaa gatactgtta aaaatggtaa tgcttgtagt aatgttattt t
//
LOCUS       DAMJYC010000213         2685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJYC010000213 DAMJYC010000000
VERSION     DAMJYC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.222"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            378..2189
                     /locus_tag="PC472_003978"
     CDS             378..2189
                     /locus_tag="PC472_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2729243.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2277..>2685)
                     /locus_tag="PC472_003979"
     CDS             complement(2277..>2685)
                     /locus_tag="PC472_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729244.1"
                     /translation="EVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1011 a          270 c          398 g         1006 t
ORIGIN      
        1 gagttaaatt gtaggtaaat agtattttaa ttatttatta aaatatagat tatgtatatg
       61 ccaaatttaa aaagagacat aaagacatta tacaaatata ttattggggt gatattttat
      121 aagaaaagaa gtgatgcagt aaattaattg agcaatttaa aaatgattta atgatagtgt
      181 tgatgaatgt ttaaaaagaa attttagaaa aacgtatatt gctgtttgtg ttggaggtga
      241 tgaatttgta gaatttatag ggtataaatg attaaactat ttaagataaa tacatatcac
      301 tgtaaaatat tgataatcat tttttataag agattgatta tttgcaaatt atatatatta
      361 caaatgaggt aacaataatg aagaaaaaat atgatttttt aatttattct gctatttggc
      421 tagttattat tattcttttt atattttctc tatatcacag tatagagcat attaaaatta
      481 taagtcacac tggtactatt cgcagtgaaa ctcaaaaggt tgttaagcaa gaattgaata
      541 atgaaagaaa tgatgacttg ataaaacgac tggataatat tctgataaag ctaagaacag
      601 gtaatggaga aaatggattt caaagatgtg ataataaaga atttcaacaa aaactaaatc
      661 aaatggattc tatgtgggaa tctatgaaga aagaaattat aaaagttaga aatggtgctt
      721 caggtgataa attatataaa ttgagtgaag agtattctgt gttgtctaat caaattgttt
      781 ttatatctga aaaacactct aatgcaaaat tgtattcttt tgctactgca ctatttattt
      841 atcttatatt ctcaacaata agtcttttaa tttgggaata ttataataaa aaaaggttta
      901 aaagaatatt ttatactgat aatttaacaa aaataaaaaa tcaggttgca tttgaaaaca
      961 gagcgattga aattttgcac aatgcatcta acaaagagta tgtactgcta aatattgata
     1021 ttgacaattt taaatatatt aatgatacac atggatatga atatggagat aaagttctaa
     1081 taatagttgc aactgttctt tctaaaactt ttaatataaa agaaacttgt gcaagaattt
     1141 gttcagataa ttttgtaata cttgcaaaat atagagattc tcttttagag gatattcggg
     1201 aaatgctaac taatgctata atctctgaat tagatatgaa tgtaactcaa actatatctt
     1261 attgtatagg agcttactta gttgaaattg ataatttagg ttataaatct atcaattcta
     1321 taatggataa ggcaaatatt gcacacaagg taagtaaaac aagaggaatt tcttctacag
     1381 tatggtataa tgaaaatctg ttaaaacagt tacaaatgga aaatagtatt tataattata
     1441 tgtataaagc gatagaaaat gaagaatttc atatgtattt gcaacctaaa tttcaaatat
     1501 cttctttaaa tgttgtgagc gcagaagctc ttgtaagatg gttttctcca gaacttggtt
     1561 ttttatcacc agatgaattt attccattgt ttgaaaaaag tggatttatt attgaattgg
     1621 acttttatat gttaaaaaaa gcgtgttcat ttgtaaaaaa aacgtttatg aaaaaaaatc
     1681 aatatacata tccgattgca gttaattttt caagggttac tatttaccaa aatagttttt
     1741 atcaaaggtt tttagatact gttaaagaat atgaaattcc ttttaagtat atagagatag
     1801 aggtaactga aagtgctttt aatgaaatat ctcaaccagt tatttcgatt ttagaggagc
     1861 ttaaaaagtt aggattttta atttcaatgg atgattttgg ttcaggatac tcttcattaa
     1921 gtcttttatg ttctctatct attaatggat tgaagttaga taaaagctta cttaaagaaa
     1981 cctttaaccg ggagaaagta tatagtatta ttcaatgtat tattgaaatg tcacatcgta
     2041 taggcatgtc tgttgtatgt gaaggtattg aaactaaaaa agacttggaa tttttaaata
     2101 cagttaaatg tgatgtagga caagggtttt atttttctaa accgatagaa gaaaaagaat
     2161 tttttaacaa atatgtaact aaaaaataat ataattattt ttattagtct atacattgag
     2221 tcattttata tttcgttaaa tgactcaatg tattgttatg tattttaaaa tataaattat
     2281 ttaaaacgtt ttttgtactt atatagaaat tcttctatat tttttgattg ttctcttatt
     2341 tcaactgctt ctatatctag taaataaagt atagaatcat ttgtaagttc aacatattca
     2401 ccatcttctg aaccgagtgc atgtctctca atttttaatc ctttttcttc ataatatttt
     2461 tttgtttcta tatatagtga ttctcgcttt tgtatgtagt ctttcacttc attatttgga
     2521 aagttatatg aatccactaa tgtttcgcca tttataaaag ttttctcggt agcaactttt
     2581 tcatcccaac aatggatttc ttcccaagta aaaaatatct cagttccatc tggaatgaat
     2641 gaaaacctgg taataatata taaataatta ttgaaattta cttca
//
LOCUS       DAMJYC010000214         2656 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJYC010000214 DAMJYC010000000
VERSION     DAMJYC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.223"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(926..1963)
                     /locus_tag="PC472_003980"
     CDS             complement(926..1963)
                     /locus_tag="PC472_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2729245.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2060..>2656)
                     /locus_tag="PC472_003981"
     CDS             complement(2060..>2656)
                     /locus_tag="PC472_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2729246.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
BASE COUNT          883 a          404 c          340 g         1029 t
ORIGIN      
        1 tagctaccat cagcagacat atctggaaaa tctaagattt tgtaagttat ataaaccccc
       61 agtgccataa tcgataaaat taaactttgc gtcattacag atataatacc tgacataaat
      121 aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt gtgtataaaa
      181 aaaccacata gtgtacaaaa ctatgtggta aaaacacgaa taagccacat agtttaatct
      241 atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac atagatacaa
      301 tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag gtacaaaccc
      361 taaaaatagt ggctataata ataataatat tttctattgt ttaataaagt attcataatt
      421 atctctccct tttaatattt taattagtca gaaaattata tgtattctcg acttgttata
      481 aattataaat tatacaataa taaaagtcaa ctgctaaatt taattaaaat actaaataat
      541 gtatactcag aatactttta aattaatgta ttgtttgcaa aaaacgttat cctaagttat
      601 aagtgaatgt ggaaatttta tcaaaatatt tactagatat ggaataacta gacatgtata
      661 tcaatcgaat atcataaaaa aaaagctgtc tggagaatat taaaattctt ttggacagct
      721 caagtagtga ttagaaagtt atataacttt cataatcagt cgtcaatata gacgactttt
      781 atggaaaatg aataacttta tatgcaatat tcaaattgct acaataagca caattttcaa
      841 agggttaggg tggataagag tacttattat ataaagtttt catattacct acttcatatt
      901 acctattttt tgaaattaat aagttttatt ttgcagattt tgcattttca tctgacttta
      961 tatactttat attatctgct gataactttt gcatgtcaag tgcttttagt gtatcttcgt
     1021 taattattat ttcagtttca tctaatgtag taactggtat atcgccaaca gattttcctt
     1081 ctaatacttc aatagcaagt tctccagcct tataacctag tttttcataa tctattccac
     1141 aacaagctaa agcaccagaa gacacagaac cttcttcaga ggctattata ggaatcttgt
     1201 tttcattagc aactttagaa actattggca tagaagaaac tattaaattg tcagtaggga
     1261 catataaaac atctattttg ccaactaaac tagaaatagc ttggttaact tcacttgaag
     1321 agctgattcc tttttcaact acttcataat tatttttctt agcataatca tgtagagaat
     1381 caacttggat ttttgaatta acttcactag tattgtatat aacccctatt ttctttgctt
     1441 ttggagtcaa tgttttaacc aattctaatg ttttatcaat tgaaagataa tcagatgtac
     1501 cagaaacatt tccacctggt ttttcaagag atttaacaag tccagcttct acagggtctg
     1561 taacagcagt cattattatc ggaatatctt tagtagcatt ataagctgct tgtgcagatg
     1621 gagtagatat agcatatatc aaatcttttt tgtcggatac aaacttacta gcaatacttt
     1681 gtgtagtagg catatcattt tgtgcatttt ggaaatctat ttttatatta tctccatctt
     1741 tatagccttt atcttcaagt gctttgatga atcctttctt tgctttatcc agagatggat
     1801 gttccactaa ttgagtaata cctatatttt tttgtttttt gctatctgtt tctttatttt
     1861 catttgaagc attggagcca tcattttgag aacatccagt aagcattgat acaccaagta
     1921 ctcctgctat aattaaactt aatatttttt tactttttat cattttattt tcctccctta
     1981 aaatttaata attatatttg ttcatatatt catatgatag attataaata tttctgatta
     2041 aaactaagat tttaatttct tatactgcat attcaaattt ttctaagatt tcttcaactg
     2101 ttaaatcttt tttttcttta tcttttacat ctaatacaac ttcaccctta tgaagcatta
     2161 tcaacctatc tccatattga agagcgtgtt tcaagttgtg agtaaccata agtgtagtta
     2221 tatttttctc tcttacgatt ttatctgtta actctattac ttcatttgat gtttttgggt
     2281 caagtgctgc tgtatgttcg tctagtaata agaccttagg acttgctaag ctagacataa
     2341 ttagtgatag tgactgtctt tgacctcctg aaagatattg tactttaata tctaaatatt
     2401 tttttaaatc caaagaaatt ccttcaagta agtgttccaa gtcattcgtt ttatgacgaa
     2461 gacatttttt taagttcaat aatttccctt tattaagtgc tagagataaa ttttctctaa
     2521 ccgtcataga aggacaagtt ccaaggcttg ggtcttgaaa aactctactt actatttgag
     2581 ttcttttata ctcagctaaa tttgttatag tagtactatc taaagtaatt tgaccagaag
     2641 attcttttat aagccc
//
LOCUS       DAMJYC010000215         2596 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJYC010000215 DAMJYC010000000
VERSION     DAMJYC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.224"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(211..1461)
                     /locus_tag="PC472_003982"
     CDS             complement(211..1461)
                     /locus_tag="PC472_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2729247.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1493..2434)
                     /locus_tag="PC472_003983"
     CDS             complement(1493..2434)
                     /locus_tag="PC472_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2729248.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          779 a          495 c          243 g         1079 t
ORIGIN      
        1 ccttataaat atacaaatac aattatatga attttcatcc tcaattatag catagaatat
       61 gactatctat atcctaaaat tatccatttc taaaaaaaag gtaactatta ttaagaatat
      121 caacttatag aatttatatt ccacatacga ttttcaatca aaaaaacaag ggctaaaaag
      181 tgaattgtgc actttttagc cctttattaa ttattcattt atttcatgta agaatagacc
      241 acatcttact ttaggttcaa accatgttga cttagctggc attatcttat ttgcatctgc
      301 tactcttatt aaatcttcaa tactagtagg atacattgca aatgctactt tcatatcttc
      361 attaactctt ttttctaatt cttcaagacc tctaattcca ccgataaaat caattctttt
      421 atctactcta ggattatcaa ttcctaaaat agcatcaata acatagtctt gtaatataga
      481 aatgtctaat gagtcaacag ggtcttctat ttctaataaa tttttatttg ctttcatttt
      541 ataccactta tcacctaggt acattccgaa agttcctttt ttgtcaggtt tatatttttc
      601 ttttccttct atttcttcta gttcaaaatt ttcttttatt ttatttataa attcttcctc
      661 gcttaaacca tttaagtctt tgataaccct attataatcc aaaaccatta aattctcatc
      721 tggtgcaatc attgctatga agtaattaaa ttcttctttt ccagtataat ttggattttt
      781 agctctcatt tctttggcta cattttctgc tgaagctgtc ctatgatgac catctgcaat
      841 gtatagataa tctaattttt caaatttatc tactaggtta tctattatat catcattatc
      901 aatcttccat actgtatgct ctatcttgtc atctgttatg aaatcatata aaggttcatt
      961 attatccata aaatcattga ttatatcatc tatcaccctt tgatgtttgt aagttacaag
     1021 tatagttccc gtattagcat cacagtattt tatgtgttta gttctatcaa tttccttttc
     1081 tggtcgagtg tactcatgtt tttttattgt tccattcaag ctttcatcaa ctgatataca
     1141 tgctactata ccaacttgag ctctaccatc catgatttgt ttatatatat atatacactc
     1201 tttgtcatct tttactagaa tttcttgttt tcgaaactca tctaaatttt ctcgcgcttt
     1261 taaataaact ttttcatcat ataaatctat attttcatct aaatcaattt ctgatttatc
     1321 tatatgcaaa aatgaatatg gattcccttc tgcaattctt ctagcttctt tactattcat
     1381 aacatcgtaa ggaagtgctg ctactttatc tactatatat ttatttggtc taatcgcctt
     1441 aaatggttta atcgttgcca ttactaattt cctcctaatt atacaaaata ttttataatg
     1501 ctacccctat tagattgttg aagtcaagaa agaaatcttg aatcacattt actatttcgt
     1561 ctcctattct ttcttgagct tctactgtag atgccccaat atgaggtgta ggagaaactc
     1621 ttggatgatt tactaagtct aaatttaatg taggttcttg ttcataaaca tctatagcag
     1681 ctgctgataa tttaccttca tctaatgctt ttagtaaatc tttttcatca actaaaccgc
     1741 ctcttgcaca gtttattaaa taagcccctt tcttcatctt atttatttct ttttcagtta
     1801 ttactgcccc cttattctta tcaaatggta tgtgtaaaga taagaaatct gcattttcta
     1861 aaacttcttc catatcacag aatttataat tgttaaagcc ttgcgcctca cccattatgt
     1921 cagtatatat aacattcata cctaataatt ctgctctctt tgcaacttct tttgcaattc
     1981 ttccaaaacc tataagtcca agtgtctttc cattaatttc tgtaccttta tactttttct
     2041 tctcccattt tccatctctc atagttacat ttgcagtatt tatgaatctt gcaagaacaa
     2101 ataattgacc tattgtaagc tctgcaacag atatactgct tgcatttgga gtattcataa
     2161 cttttatgcc attagcttga gcatatttaa catctatatt atccagacct actccacctc
     2221 ttactatcaa cttcaacttt ttaccctcta atgctttatc tattacatct ttagtaactt
     2281 tagtagctga acgtactaca agtacatcaa catctttaag tttatctcct aaaacatctt
     2341 tttcataaaa ctgctctatc acattaaaat ctaactctct caattttctc gctgcttctt
     2401 tctctattcc atcagtaact aatatattat acataacaaa ctcctccaat ttttattcac
     2461 ttaatttttc caatttttat tttttaaagt attttcatca ataaaaaact ttacttaatt
     2521 taactttctg tttatataat tttcaaaata tttttttatt aaagtcatat caaatttttc
     2581 tattgtttgt taaaaa
//
LOCUS       DAMJYC010000216         2549 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJYC010000216 DAMJYC010000000
VERSION     DAMJYC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.225"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            85..369
                     /locus_tag="PC472_003984"
     CDS             85..369
                     /locus_tag="PC472_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729249.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(526..699)
                     /locus_tag="PC472_003985"
     CDS             complement(526..699)
                     /locus_tag="PC472_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2729250.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1140..1355)
                     /locus_tag="PC472_003986"
     CDS             complement(1140..1355)
                     /locus_tag="PC472_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729251.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1366..1833)
                     /locus_tag="PC472_003987"
     CDS             complement(1366..1833)
                     /locus_tag="PC472_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2729252.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1034 a          251 c          310 g          954 t
ORIGIN      
        1 tttgaagaat atggaaaaac ttgattagta cattattaat tagggtataa aatggtgtaa
       61 atagaatgat aaggggtgga tgaaatgctt gtatataata aaagttttta tcctaatgac
      121 atatttccaa gattagattt ttcaaaaata aaaaaacagt taaaattgat agataatgac
      181 ctgtcagatt ttggaagcat atgtataata gaaaaagaac attatacaat aagtgtaaac
      241 agtataggtg aaataaatgt gtattatgat ttagagtacg aaaataaggt gtatagaata
      301 gtttatgaga ttgaaaagtt atttaaatct caagttggaa ggtttagcat atctacatac
      361 agaaattgat aattaaaaaa gtagaaatta aaaaacttaa tactaaagat atagataaat
      421 atatgaggta acagctagag agagttgtta ccctttttaa atactaagta tatattattc
      481 ttattaattt tttgtaaatt cactaaaaga cctttttata gaatattatt ttcttttttt
      541 attaaatagg taaatcagta aaaatgcaat tccagttcca attattattc caatgatatt
      601 ttctataatt aattttttta atataaaagt aaataataaa aatgctatac ataaacctaa
      661 aatatatcct ttaaaatagg ttgaaagttt tcttttcata taatcaccaa cttgtatcag
      721 atttagttta cttaattaga gtataacagt gttgtaactt caaagagtat ctaaaagatt
      781 acaaatgtta gataaaatca tcaataatgt aatttataac acgtagctat aaactatgca
      841 ataatataaa tggaggtata agtaaatgaa aaagttccat gttttgctaa cagtttaaat
      901 aaatattagg tagaaaagaa tgtatatagg aatctgtggt aaaattatca atttaaaagt
      961 attatttagt gtgatacagt tcttataggt aatggataac aaatagataa aggaacagtt
     1021 tctttcttag aacatatttt gttatgactt taaacaaagt taatattaat gtcaataata
     1081 tagtaaaaga ataaaaatca tatagtataa taaaaaagag tgtttctaaa cactcttttt
     1141 taatcatttt catcttctac atattcaagt atatcacctg gctgacagtc taaagctttg
     1201 caaatttcgt ttagagtggt aaatctaata gcttttgctt ttccagtttt taaaattgat
     1261 aaatttgcat ttgtaatatc tattttttct gccaaatctt taagtgatat tttccttttt
     1321 gccatcatta catctaggtt aactactatt gccataacat cacctctata tcgttaaatc
     1381 attatcttct tttattttta tagctttatc aaatgtttct gcaattacaa agcacattag
     1441 tgcaaatata aaagagataa tcataagtgg tgagataccg ccactatcag ttccaaaaat
     1501 tcttatatta ttagtattta aattataacc attgatacat tcaattatag tgtttatcaa
     1561 taaacaaaat cccattacct taaacctttt tacattttca agtacgaatg gggaatactt
     1621 agtactatta attataaata tcaagaataa gatgactata aagtagtcag aaaaatatag
     1681 tattcttgca attaaagcga ttactttgtc agaactaaaa tcatataaaa acataggtat
     1741 catacataag aatattatac cagcaactcc taaaagtgat aatagaaaat atagaaatac
     1801 tgataatata ttactaaaaa attttgtaat cataaaatat catctccttt atataaagta
     1861 tattattgat tataatatac aatttattga atttcaataa taaattattg atataatata
     1921 taaaaccatt gtaatataca ctaaaaataa gaatgtataa ggtggtaaat ttatataaaa
     1981 aagaatatga gttgtaaaat attaaataat attgtactaa ttggaataat tttaacttta
     2041 ttatagatta ttttaatacc attagtactg atggtgtatt ttaaaattgg tataagcatt
     2101 acaaatacga atatgccaac tgtaatatgt aatataagta gctatataca tatgtgctgt
     2161 cccatatatt gtatcattgt ttatattaaa aagattatgt aaattggtat caatcaataa
     2221 atcattttca gaagaaatac ctaaaaactt aaaaaatacc tatatgtgta tttagttaga
     2281 tttgagattt ttacatttaa ttttatagtg acaatattat attattttta tgatgtctat
     2341 ttatatgcaa ttactataat atcagaactt atagtatcat ttgtttcatt atcaataagg
     2401 ttttttactt tagttttgtc aattttatta aaaaaagtta ttgatataaa agataagaag
     2461 gataaaacta tatgacggat ttcaaacata agagtggata tttatataat agatatttta
     2521 tagttaattt tattagattt tatttaata
//
LOCUS       DAMJYC010000217         2514 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJYC010000217 DAMJYC010000000
VERSION     DAMJYC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.226"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            404..2299
                     /locus_tag="PC472_003988"
     CDS             404..2299
                     /locus_tag="PC472_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2729253.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1097 a          245 c          452 g          720 t
ORIGIN      
        1 gtattagaat aatttttaat tttaaactct aatttatgat gatttttata atatgttata
       61 tattttaata tatggagata gatattaatt tagtgatata tatttaagta ataagagtaa
      121 agcaaaaatt agtgcatagt agtatataaa aatggagttt gaatactttt gttagtatat
      181 gattgtttag tagaaaatca ctataaaata ggatttctat ctttgagcat aattatagag
      241 agctaagtag ggggaagggt ttcaagaaga tattattagt gtttgtgtta gtaaattatg
      301 taaacacata aaatatattg aaaaataata ttatatataa tattatttat gtgtgggaaa
      361 tacgaatata aaaaataaaa aataatattc ggaggacata aatatgttaa gcaacaaaaa
      421 gagaagtatg gcaatagtaa tggcaggtgc tacagttatg agtgcagcag caccaatatt
      481 cgcagacaat actgtgacag aaaatgttga taaaaattac acagtaagtg cgaaagattc
      541 tgctaaacta atcgaagagg ttagaaaagc attagaagtt aagtttgaag atacaaaagc
      601 aggagcaaat gtaaatgata gagtttacga tataaaagta gacaatgtaa acttaactaa
      661 tgcaactcaa ttacaaaata aaataaactc tttaacagaa gggcaaagtt taaaagttac
      721 tattcaagat aaagggcatc aagtgttagg tggaaaagta gttgactata aaattgaaaa
      781 ctacaaaact gctcaagaaa tagtagatgc agttaatgct tacaatgcaa ctttagcaga
      841 agattctgat aacaagttaa cagcaactat aaaatctaca aatacagttg aagtaaaaag
      901 agcaaaagat tcagcaaatg ttattacatt aaatgtagga gaccaacatt tagatttctc
      961 aaaagtgata acatcagaag aaggtacttt tgaaggttat gaaaagagtt atagtgacat
     1021 agattcaaaa gaattacata cgataacagt taaaaacgca gatttacaag acatatcagc
     1081 agaagaatta tttgatggaa ttagattaac tacattagga agagaaatag ttaataaagt
     1141 taaaaatgga tatgctttaa catttgaaaa tgaagcaatc cttactcaag aacaagaaga
     1201 tagtgatgat aaagataaac cagaaaaatc aagctttgat atagttttaa gcaaggcaaa
     1261 tgaaaagcca gaaacaataa gtgtttcaag taaaaaccat aagttagtta gagacttaca
     1321 caaagtatta actgatgtaa aagatggaaa agaattaaaa gtagaagttt tatctggtga
     1381 ttcaagattt acaacagcag tagaagttag taaagagaga ttcaaagatg gagaagcaga
     1441 agctataatt ttagttggag aagatgctat agttgatgga ttagcatcag caccacttgc
     1501 ctctcaaaaa aatgcaccaa tattattatc taaaaaagat tcactaccat cagaaataga
     1561 agctgaaata ttaagagtac ttggaagtaa tctatcttct aagaaaatat atatagtagg
     1621 tggagaatct aaagtatcaa aagaaactga agaaaaactt tctaaattag gtgtaagtaa
     1681 agttgagaga gtttctggag aagatagatt tgaaacttct ttagaaatag caaaacaatt
     1741 aaaagataca tttaagactg cttttgtagt aggtggaaat ggagaagctg atgctatgag
     1801 tatatcagct agagctgctc aatttggtgc tccaataata gttacaggta acgaattaga
     1861 tgcaaatgct gaaaaattat taaaaggaaa agaattagaa atagtgggtg gagaaaattc
     1921 tgtatcaaaa gaagttgaag acaaattagt tgatatagat ttaaataata aagttgaaag
     1981 attagctgga gaaaatagaa aagatactaa tgctaaagta atcaataaat actatgcagg
     2041 tgcaactaaa gcatatgtag caaaagatgg ttatgtaggt ggaaatggac aattagttga
     2101 tgcacttaca gcagcaccac ttgcagctag ttcaaaagct ccaatagtat taactacaga
     2161 agaactttct aaatcacaag aagaagtagt tgagttaaga cttaagaatg ctactaagtt
     2221 agtacaaata ggtgaaggaa tagctaaaaa cgctattgaa aaaatagcag aaaaaataaa
     2281 cttatttact aagaactaat aagatatagg aattaatatt aaaagtttat ttagataata
     2341 tatgattata caatgcgtta atatgtaaga catagtgtag atattataaa atatacaaag
     2401 aacaagataa aataataaaa ataaggtgtc tcatttagaa ttagattgag ataccttatt
     2461 ttattttttg attataaatg ttgagaaatt catattgcaa aaattattat aaaa
//
LOCUS       DAMJYC010000218         2347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJYC010000218 DAMJYC010000000
VERSION     DAMJYC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.227"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            37..1857
                     /locus_tag="PC472_003989"
     CDS             37..1857
                     /locus_tag="PC472_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2729254.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRYETSLLIAK
                     EIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDELEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVYKIGGGLTS
                     KVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIKEKDYTLNT
                     SLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVE
                     VFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPKKDYLYVLRNNDMP
                     AVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT          977 a          268 c          391 g          711 t
ORIGIN      
        1 agtgttaagt ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gatatgccat tggatacaac tctaaattat aatcaatcaa ctgagtcata tgaaggaact
      121 atcaattata ataaagaccc agaatattta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac aaacaagaac tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac gcaggaatgt ataattgaag atataacttc tagaaaagat
      301 gtaaataaat atttaagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tacgaaacag cagttaaaat aagtaaagaa ggctggaaaa atggttcaga taaggtagtt
      421 ataataaatg gggatgtaag tatagatggc attatatcaa ctccactagc aactacatat
      481 aatgcgccaa tacttttggt tgaaaaaaac aatgtaccta atagtgtaaa atcagaatta
      541 aagcgtttaa atcctaggga tgtaattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtctaaa gggctctaat
      661 agatatgaga catctttatt gatagcaaag gaaatagata aaaatcatga tgtggaaaaa
      721 gtatacataa caaatgctaa tggcggagaa gtggatgcac ttactatagc agcaaaagca
      781 ggtcaagaca agcaaccaat tatattaact gataaaaata gtattacaga caatacatat
      841 aaatggttaa agagtgagga tttacaaaat gcttatttta taggtggccc tcaaatgata
      901 tcaacaaatg ttataaataa ggtaaatgat ataactaaag ataatgttac taataataga
      961 gtatatggag cagatagaca cgaaacaaat gcaaacgtaa taaaaaaatt ctatacagat
     1021 gatgagttag aggctgtttt agtagctaaa tcagatgtac ttgttgatgc tttagcagca
     1081 ggtccattgg ctgccaactt aaaatctcca atacttataa caccaaagac gtatgtatct
     1141 gcataccata aagataattt agaagctaaa tcagctaata aagtatataa aataggagga
     1201 ggattgactt ctaaggtaat gaactctata gcatcatcat tatctaaaca caatacaact
     1261 ccaacagaac caggaaatag tgggggtaag acagttatga ttgacccagg acatggtggt
     1321 tcggacactg gaacaacagg taaaccatta gggggtatta aagaaaaaga ctatacatta
     1381 aatacttcac ttgcgacaac tgaatattta cgttcaaagg gattcaatgt aataatgaca
     1441 agagatacag ataagacttt atctcttgga aatagaactg ctttatctaa ctctttgaga
     1501 ccagatttat ttacgagtat acactataat gcatctgata caactgggaa tggtgtagaa
     1561 gtattttata agcttaaaga taaagatgga ggaactacta agactgtagc tacaaatata
     1621 ttaaatagaa ttttagagaa atttaatcta aaaaatagag gtgcaaaaac aagaacacta
     1681 tcaactgacc ctaaaaaaga ttatttatat gttttaagaa ataatgatat gccagctgta
     1741 cttgtagaat gtgcattttt ggataatgag aaagatatga gtttacttaa tacatcaaat
     1801 aaagtaaaag aaatgggtac acaaataggt aaaggcatag aagattcatt aaaatagtct
     1861 acaatgagag catagaaaaa tttaaaataa attaatctgc aattttaaat ttaaactaaa
     1921 gctataagat aagaatgtaa ttataatagt aataaagact gtatatacat tgtgcatata
     1981 agtgcgaaat gaggtatata cagtcttttt atgattaatt gtaaaaggat ttatattata
     2041 tgaagaattt acatatctta aggctacagt taattgttaa gagttggagt attcatttgt
     2101 aatacacaat ataagcttaa ttactatatt ttattgtgtg tttgtgtgtt ttttgtggat
     2161 ttttatgtca ataaatgata tagtatagat aattaaatgt attaaatata tttaattttt
     2221 ttattttttg agttttataa gtttattttt atatattact tatatattat ttatattttg
     2281 ggttagatac actttgacat attttcaaaa gttaaaagat taaaaataaa gtagaaattt
     2341 atgttaa
//
LOCUS       DAMJYC010000219         2335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJYC010000219 DAMJYC010000000
VERSION     DAMJYC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.228"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            41..958
                     /locus_tag="PC472_003990"
     CDS             41..958
                     /locus_tag="PC472_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729255.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1100..2281)
                     /locus_tag="PC472_003991"
     CDS             complement(1100..2281)
                     /locus_tag="PC472_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2729256.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          752 a          396 c          305 g          882 t
ORIGIN      
        1 atctatacat ttaaattaaa atataaaagg agtgtattta gtggaaaata ataaatgtag
       61 agaggacttt agatttacac aagaatatga ggaagattat ccaaatacaa atgaaagata
      121 ctatgaaaat tatcaagtag ctgatagata ctataattat ccaaataaat ataaagaacc
      181 taaaataaaa caatgttgtt gtaaaaaaag tatgagagaa gcattagaac ttctaagata
      241 tgatgctcta agaccttttg taaactttaa tcaatttgct tttatctcag atttctttat
      301 agtaggtgct aatttggtag gtatagatct ttcagctcct ccaaaagata atttatctgg
      361 acttgatggt acttttgaaa gattttctgc ttgtaactgt gatttaatag atatagctgg
      421 tagagtatct tatcctattc cagtcccttt aactcttgag ggattaatta atactatagg
      481 aactatacca ggagtagctg aattaattgc acttattgat gcagttattc ctcctacgat
      541 agaccttggg gctatattag atgcaatact tgctgctata attgatttta tacttgctgc
      601 atctactcca ttagcaaacg tagatttagc atcattgtgt aatcttaaag ctgttgcatt
      661 tgatattaca cctgcagatt atgaagattt catagcatct ttaggttact atcttgataa
      721 aaaacattac aaagaatgta attgcaactg cgattgtgat gattgctgtt gtaataaagg
      781 tatcctagat aatctttata tgtcaaatat aaataatcaa gttactgtag tagctggtag
      841 tttggttcta actggtgttg aagttctagg taagaaaaat gatgttatag tacttggaaa
      901 ttctaatgat tcaagaatat actttgtatg tgtagattct atagattata ttgcataaga
      961 atgattttaa agagttatat ctaaatataa aatagaatct attttctaaa gtataaaaga
     1021 tttaattatt actaataaaa aagagtgtct ataattattt aaaaatttct ttagacactc
     1081 ttttttattt ttatactatt tatctaactt ttttaccaag tttaaatact gctaacacat
     1141 ctgcaagtac gtctatattc tctactggat ttccatttac aactataaca tctgctttct
     1201 ttcctttttc tagagttcca taatttttat caattcttaa aagttcagat gctgtttttg
     1261 tagcagcaac tattatatcc atattatcca taccataatc tgacattagt ttcatttcat
     1321 aatatgtttt tccatgttca ttataagaag ttcctgcatc agaaccaact gcaattttaa
     1381 ttcctttttt atatgctttt aggaatgact ctttgtgtgg aatggcaaca atctcacact
     1441 ttctaagagt agcttcagtt attccacttt cttttcctgc atttagaata aaatatggtg
     1501 cagccacagt tggaactaag taagttcctc tttcaagcat caaatcaatt atttcatcat
     1561 ctaaataaac accatgttct atagaagtaa ttcctgcaag cactgcattt tttataccag
     1621 ttcttccttg tgcatgtgtg gcagttattt tacctgcttt tatagcttca tctacagcag
     1681 ccttcatttc ctccaaagtt aattgtggtg accctggctc aactccatca gttgttacac
     1741 ctccagttgc cattatcttt acacaatcta ctccattttt taaaactgtt ctggctgatt
     1801 ttctacattc atctactcca tcactttcaa taccaaaata atatccatgc cctcctgtca
     1861 tacatataac tggacctgaa gtaactatac caggaccatc tatagaccct ttgtctataa
     1921 gatttttaag ttgtacatcc acatatttgg gacaacctaa gcttctaata tatgttactc
     1981 ctgaatccaa ataagcatca agttgtttta tcgtatttac tacaagctct acatctgata
     2041 cattatcata atatatcttt ggatttccaa ctggctccat tgtcaagtga acatgacaat
     2101 tcataatgcc tggcataaca gttttatcta agtaatcgta cacatcatat ccatttaaat
     2161 catctcttat atcttcaatt tcaactatag tatctccatc aataattata cccttatttt
     2221 gcataatact atttccggtt ccatcaatta aattccctgc tttaatatat tttttgttca
     2281 tcttaaattt ctcccccttt taagtacaat gtattattta tttattattt ctaat
//
LOCUS       DAMJYC010000220         2304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJYC010000220 DAMJYC010000000
VERSION     DAMJYC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.229"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            160..300
                     /locus_tag="PC472_003992"
     CDS             160..300
                     /locus_tag="PC472_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729257.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(969..1289)
                     /gene="rhaM"
                     /locus_tag="PC472_003993"
                     /pseudo
     CDS             complement(969..1289)
                     /gene="rhaM"
                     /locus_tag="PC472_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
     gene            1475..1756
                     /locus_tag="PC472_003994"
     CDS             1475..1756
                     /locus_tag="PC472_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729258.1"
                     /translation="MKDETLEDILKQKTQAERYILEYIVTKNVKKLDNKKIVSILHTI
                     LCWEEINGTIVLTDKNILSLDEDIMISKHRRFIDIPKHKHNFIEFHLMS"
     gene            1880..>2304
                     /locus_tag="PC472_003995"
     CDS             1880..>2304
                     /locus_tag="PC472_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDF2729259.1"
                     /translation="MRQYIHDKLREITEEEKNILEGNYIIDKSIYTDNSQFIIDSNKL
                     LNIDELIHIRKHTRFAQFPKHKHNYIEFNYVYRGKLVQTIDEYKINLKQGELIFLNQH
                     VIHEIEASDEEDIIINFIIKPEFFDYIISLLENDNKIMN"
BASE COUNT          966 a          249 c          286 g          803 t
ORIGIN      
        1 ttaattataa aaaataataa taaagacgat aagtattaaa attttatgtt tccaatattt
       61 atgatataat taatatggca aggaataaat atttgaaaaa gctatgagtg gtgtttccat
      121 ggaagtttct tcactttctt atgagaggag gtgaaaacta tggaaaatct aatgatgagt
      181 attataactg gtgttatagc cagttacatt tacgataaat taaagagcca ctctgagcga
      241 ccaactaaga gtggctggga acttaatata aagttccaga aaagtaaatc ttcaaaataa
      301 tttgttgaag gaaacatcac tcaaagttaa gtaaaatatg tttccttgct tttattatat
      361 cacaaactaa aaatattaac acaaaatttt gtgaatatat acttattatt gttctaaaaa
      421 aatatgtaaa taattgattt gatttcatag aattattgtt gtaatgttta gttaaatata
      481 cacaatctca attaaagaag ataagtacta taaattcata tcaaaaaaat acataaactt
      541 ttaagttata attttataaa aagtctttta gtatcattat ttcaagagga attatcatat
      601 gaaaaaataa cttcattaga aaagtggata aaaaactggt aagaagatga ctaaagatta
      661 catatactat tacttaaatt aaaaatttaa tataaacaac acaagatata tcaattattc
      721 taaagttaag ttaagtagtc ttactgaatc atctttggga ataattgttt caatgtctaa
      781 tagaaatacc acttgtttat aacatcttta gctactataa tcgaaatatg tttttagttt
      841 ttcacagaac aattatcaca tagactagag gtttttagga atcacttatc ttttttatac
      901 aaaaaggaga taattcaaat ttttattttg atatatcccc attttgtgat ttataataat
      961 ttttgtcctt attttaaata aaacatagat ttaagttcta tagatacagg gctattatcc
     1021 tcattagttt ccattatatc agacatataa ttccaccatt ttttgcagat tgctgtctct
     1081 gaacttttat cccatagttt ggcatcttta agttctatat atccatataa ataattagtt
     1141 tcttcatcta gaaaaataga atagttacta ccaccatatt catgaatcat atctatcatc
     1201 tcagatcata attcatcatg acgtttcttg tattcatttt caaatccttt aaaaagcttc
     1261 atcttgaaac atttgcgtac tgtattcata aaaaaatacc tcctaaattt taagtattta
     1321 cctataaatg tattttaaaa caaaaaaagg tatttttaaa tgtcataatt taacattatt
     1381 actgtcaaaa actaatatga tacaattaga taaagaatat ttgtaatata atttaaaact
     1441 gtaataaagt atagctaaat ctggggggat ataaatgaaa gatgaaacgc tagaggatat
     1501 acttaaacaa aaaacacaag cagaaaggta catcttagaa tacatagtta ctaagaatgt
     1561 aaaaaaacta gataataaaa aaatagtgtc tatattgcat actattttat gttgggaaga
     1621 aataaatgga actatagtac taacagataa aaatatactt tctttagatg aagacattat
     1681 gatatctaaa catcgtagat ttatagatat acccaaacat aaacataact ttatagagtt
     1741 tcatctaatg agttaatata taatttttta tcaggaacta tacacaaagg aaaaagatgt
     1801 gataattatt atatatttaa tgctaaaaat aattttaaat tgaaacaact agtagttgaa
     1861 ctaactaagg aggtagctca tgagacaata tattcatgat aagttgaggg aaataacaga
     1921 agaagaaaaa aatatattag aaggaaatta tataatagat aaaagtattt atactgataa
     1981 ttctcaattt ataatagata gcaacaaatt attgaatata gatgagttga tacatataag
     2041 aaaacataca agatttgcac aatttccaaa acataagcat aattatatag aatttaatta
     2101 tgtttataga ggaaagttag tacaaactat agatgaatat aaaattaatt taaaacaagg
     2161 agaattaata tttttaaatc agcatgttat tcatgaaata gaagctagtg acgaagaaga
     2221 tataataatt aattttataa taaaaccaga gttttttgat tatattatat ctttgttgga
     2281 aaatgataat aaaataatga actt
//
LOCUS       DAMJYC010000221         2253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJYC010000221 DAMJYC010000000
VERSION     DAMJYC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.230"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(137..1704)
                     /locus_tag="PC472_003996"
                     /pseudo
     CDS             complement(137..1704)
                     /locus_tag="PC472_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1679..1822)
                     /locus_tag="PC472_003997"
     CDS             complement(1679..1822)
                     /locus_tag="PC472_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729260.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2040..2222)
                     /locus_tag="PC472_003998"
     CDS             complement(2040..2222)
                     /locus_tag="PC472_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729261.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          705 a          310 c          254 g          984 t
ORIGIN      
        1 tacaatatag ttctaaaaaa gaatatatta gaagaagata atatttaaat aaaaaatacc
       61 ttataaagtt aattttttaa tactttataa ggtaaataca tattgtattg tggggataaa
      121 tttaaatcat ttgatactaa tcaacactaa gaaccaataa ctctctatca tcttgagtaa
      181 ttgtatatat tcttaatttc ttgtatgtca taatattttc tccacctgta aaataactcc
      241 taagattaac acaatcagtt ttaggttgat ttgtatctcc taaataatta cgcaattctt
      301 tatctttagt ttcaatatct ttaaatccaa aatattgtaa agtagcttca actctatatt
      361 tttggacata atcgagagct tctttattag tttatgttgg tgtaaaatac ataccattta
      421 tataagcctt tattgtactt ggattaaatt taaaatctgc agaatatatt ttatgtgtaa
      481 ctattatttc ttgaacagtt ggaattttaa cttctggttc atcaagtatt tcaggagtac
      541 tatttagctc tgtaatagat aagttatcta agtatattgt atcactatca attaatgcta
      601 tatttatatt agaagaatct ttttcagtag tttcaaattc ataactaaat tttgtatatc
      661 cttgttctag taccaaataa tccgtttttt cttcttttac ttttatattt acaattattg
      721 aattatatgt taaaggattt tttgaatttt actaaaaaca taacgtttat aaggttttag
      781 ttcagacata ggtattttaa tcttcgtctc accatttaat ctatttgctg agccttttaa
      841 accatcttga tttgtagtat taacattact ccatatacta gggtaattat tgtaatcatc
      901 aaaattagta aagtaagttg gtgtttttat aagtatattc attcctcttt caagtttaac
      961 attacatatt tgtttatcag acaaagtttt taaactgtct ttaatcttat tgcctatatt
     1021 atcatcaaat atgagttcaa cacaactttc atctattggt atattattaa aatataacaa
     1081 accatctttt ttagtagtgc caaaagtttt ttcaattgct tctccaattg taagttcagg
     1141 tgttttatct tctgggacct gtaaactttt agcagtaatt cttcttttgt aagattcatt
     1201 ttctgtatct aatataatag atacagaaat actgtcaatt tgacctatat cgtcttacca
     1261 actatttcat tctgttacta tttgtccaga accatttttt gtaccaaaat ttccacttac
     1321 ttgtgttgtt tctaatttaa tttgctttcc atcatctaat ttttttaatt aattataatt
     1381 tataggaatc agtctagaac taaattgata cattgtgtta agagccatag gtgaaaatcc
     1441 ttttttgaga taagtatttc caggagatag attattgcca atttgatttt cttgtgcttt
     1501 gatagttgat aatgcatctc catctaacac taaatttgtt gttggtgtca ctttgtacat
     1561 aggtgcagta cctgtgttgt agtatctaat atttgcattt atatatgcag attctccttt
     1621 gtttatactt aatccagtat ttcatgatta tccattgcta ttttgaacag cagttgaatt
     1681 atctgttgta tggtaataat ttgtcttgta tagtttcttt aaactcatta aatttttcta
     1741 atgaaatttc gttttgattt tttgttctta ttactttatt aaatgcaaat ttttctggag
     1801 attcaaaata atatacatac ataggtttat ctattttatt ttttgatata gcatttcgaa
     1861 gttctctata ttctttttct caagaatttg cttctatttg ataatcataa aaataatttc
     1921 ttgtctgaga atatttgctt atctctatag aatctttttt atatgatcct aatacttctt
     1981 tttcagattt ttcaagtttt tgatcttttc tttctcattc tttagccttt tctttatctt
     2041 taaaaaaatc cttctgatct ttctatagga gccttgtaag tagtattaca aacttttcca
     2101 ctatagcttt gtaaatcttg tgcataaatg ttattaggta acaaactacc taaagttaga
     2161 tataatatca acaatataga tatacaatta ctaatacttt tatgtttcct aaattttttc
     2221 atttattctc cctacaaata ttagtttagt taa
//
LOCUS       DAMJYC010000222         2218 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJYC010000222 DAMJYC010000000
VERSION     DAMJYC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.231"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(64..978)
                     /locus_tag="PC472_003999"
     CDS             complement(64..978)
                     /locus_tag="PC472_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2729262.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1810..1992)
                     /locus_tag="PC472_004000"
     CDS             complement(1810..1992)
                     /locus_tag="PC472_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729263.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          806 a          279 c          278 g          855 t
ORIGIN      
        1 tacagttaac tgtaccttta ttttatctaa atatgcaaag attattttaa aagtaatttt
       61 taattacatg tttttataac tctccaatat tctaatataa tgatttgcct ctcttaaaac
      121 atggtctgct aaaagtggta atattataga ttttatctta cactgttcta ttcctgatgc
      181 tcctgcttct ttaaaatctt taaactcaac agtttcattt agagtttctt ctgtaatgtt
      241 cttgatatta gaatcagtca tttcgtttgt tttttcaatt aattcattaa attttatagc
      301 aaaatcattt gaagtattta ttagttcacc ttctgagggg tctagtaatc ctctcataaa
      361 cagagcatgt tccatcataa tatggtccca gaataattct atttctttag cgttttttga
      421 ctcaatatct attttatttt caaggtcaac tacataagaa cgatataaat ttgcttcatg
      481 tattatatgt tcaagaagta gagggtagtt tgatgtaaat atagtacatg atagtacacc
      541 atctaagact ctttctttaa aattaataag cccatcaagt aatcttattg catcagagtt
      601 aagttgagct acatagttca ctaagtcttg accaacttgt gggtctacac cactttgtag
      661 atttaattct cttgtagtga tgtttttgtt tatttctatc cctgtaaact cttctgtttt
      721 ttgttctgca actgatgtga gagtagttac aagttcttct gaatataata tatcaggtct
      781 aattatacca ttactagcac taacagtgta tgataataaa tcttcaaatt gctttttata
      841 gtggtcagct tccatagcaa gattataatt tttatttgtg aatcctgctt ctaaaaaaag
      901 agcatgctct ttcataattc ttgaaaaaaa taaatgtaat tctagtgata aaataacata
      961 tttttgatta tctatcataa tttcctccct ttagaaagta tcctatttat aatactttat
     1021 tcgtaatatt taaatttatg tatattataa ataaattaac taaattcaat tgctactaat
     1081 atcaacagtt tttattaagt tatgggcgaa aaaattatct tactgtgttt cttattagat
     1141 atagatataa tgatttaagg tacttgttgg ctattattta ttaagagtac aagtaatact
     1201 agaaattttt atgagaatta tgtaatttat aagtattatt ttgatacaaa acaaatgttt
     1261 tttgtaaaac aagaactttt atagcaaaaa atgaataaat atgaagcaag cataatttta
     1321 taccacaaca attattaatt ctaatagctg cacttaatgt tttaggtatg ggatttaaaa
     1381 agtataaaaa attaagtgat aaatatattc cattatattg cttgtgcttg gagtagtatt
     1441 ttctgtatag atgttaggat ttaatccaac ttctatttta caggggattc tatgctggag
     1501 tgtttcaatt ggattaaatt aagtatgcaa acaatttcaa gacaatgata aataattgga
     1561 tgtgtgatag gaatatcaac aaaaaacatt attaaaaaga attattttat ttataaaata
     1621 aaacaagtaa taagaataaa taaggagcaa ctaaaatgat ttaaatatac tagaattaaa
     1681 aagagaatgt aaagttgtct agtaatggat gtaaaatatt ttaaacgata agctatgaaa
     1741 ttgatatatt aaaatgggta agaagaatat tatataggat tcttcttacc cattttaata
     1801 aatttaattt tatgccaaac cacattcgca tagtagaatc atcactctat ggagacataa
     1861 ctctacaaca tcttctccat attttttaat aaaatcacca atttgagttt ctatgaatga
     1921 atagtattct tttaaactag caatatatgc agtcattata tgaacacatt catcaatctt
     1981 atctttaaac acaactaatc cccctaaaaa acgaaaaata aaaatattca gtaaatagca
     2041 gatattattt cactcataaa tctatatagt atgatatatt gcttagatat attttacaag
     2101 tggatatgta aaactggtta ttataatcgc aaaactggat aaactttcat tcttaaaaga
     2161 aaatttatcc agtttaaagt ttaattatga atatttgagt tagtagatat ttaatata
//
LOCUS       DAMJYC010000223         2203 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJYC010000223 DAMJYC010000000
VERSION     DAMJYC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.232"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(124..1416)
                     /gene="brnQ"
                     /locus_tag="PC472_004001"
     CDS             complement(124..1416)
                     /gene="brnQ"
                     /locus_tag="PC472_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2729264.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          735 a          371 c          245 g          852 t
ORIGIN      
        1 atttatcaaa taggattaat aatgttcttt tatctacaat aatttaataa ttcttttaaa
       61 tataaaaagg aattgcctat ataatttaaa ggcaattcca taattatcaa ttagttttta
      121 ttattatgca gttttactgc ttttagatgg aataaagttt cctattattc ctgctattac
      181 aactggaact atccagttaa atcctaatga agcaaatggt aagctattaa ctagaggaat
      241 ttttacacca aaacttgttg caactgttaa tacacttact aataatgcca tgtatgcagc
      301 acatttgaac acgttgtcat ttttaatttt attactaaat aatgctagta taactagagt
      361 tatagttgct ggatatacca tacttagtat aggagaagag aattgtatta ttgtacttac
      421 tccaaagttt gatatgattg cactaaatac acaaactact ataactatat gctcatactt
      481 taattttcca tctgtaagtg ttgagaaata ttgaccagct gctgaagaaa gacctatagc
      541 tgttgttaaa catgctaatg caactataat agccaataaa attttacctg tagttccaag
      601 taaagctgct gttattgcaa ctattaaagc tgtttgttga acatcttgtc catattgttg
      661 agacacagtt gctccaagat aacataatcc accataaact aatgccattc ctattactgc
      721 aattacacct gcacttattg tggctttcat cttttctttc tcacctgtat aacctttagc
      781 tattactgac atgattataa ttgatgcaaa tacactagct gccaatgcat ccattgtttg
      841 ataaccttga gttatacctt ctgcaaatac attttgtatt aaagaactgt ctcttatttc
      901 tcctaatgga gatacgatac ctttaactat taatactgcc aaagctataa gtaaagctgg
      961 agttaatatt gacccaacta tatctacaac ttttgatggt cttattgtta aaaccaatac
     1021 tagagcaaag aatactatag agaatattac tgggcttatt ccagaaccta ttgtaggtaa
     1081 tatacccatt tcataagttg ttgctgctgt tctaggtatt gctaaaagtg gccctataca
     1141 gattaccatt gcacatccta atgcaatact taatcctttg cctgctcttg agaacatctt
     1201 cgatgtgtct ccattgaact tagttgctgc tataactgct aataatgcca aaccaccatc
     1261 tgcaaaaagg aatcctatga aagctactag ccaactactt ccagataaaa ctcctagata
     1321 tggagggaag attaagttcc ctgcaccaaa aaacattgca aatagtgcaa atccaattac
     1381 aattacatct ttaaaattga tttgtttctc acccatttaa agcaccccca tttgaatttt
     1441 actacctttt actaaaaaat aagtccaatc tcttaattaa atgaaaacct tttgacgaga
     1501 ttttatttaa ttatacattt ttgacttaaa tttgtcaatt atgtttcata atttataaaa
     1561 tcttcacaat atttaataca actttcttaa ttaaacaaaa tcaacctgta tactttaaac
     1621 atagttggtt ttttgttttt tctctaattt ttcttgataa aattaactat tccgaataat
     1681 taaaatatta agacttttta gattattcga tttattatta agttgtattg ctttatatac
     1741 tgtatatatc actctccttt tttacattca tataatcttt ttaattcaat ttaaatttta
     1801 aatattttgt gtttattata taatctgaaa aaaaagcagt caatagcatt tttaattttt
     1861 ttaattttca aaattatgat tcttaaaaaa tcgttttcct tttatcataa cacctattta
     1921 cgttaattct tgtagtattt tgtatttatt ttgctaaatt gacagttaac tttattcaat
     1981 ttttattcta cttacgtaga tttgcctctt gttttttact cttaaaacca ttttttctga
     2041 aaattcctta ttatgattta tcaacttatt tttcttcata acaattactc tctaacttcc
     2101 aacttactta aactttgcag ttgctagtat tcacgtttac atacttatat gtcataatta
     2161 actaaaaatt cttaatatat ctctcaatga aaacaattta ctc
//
LOCUS       DAMJYC010000224         2083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJYC010000224 DAMJYC010000000
VERSION     DAMJYC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.234"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(92..631)
                     /gene="scpB"
                     /locus_tag="PC472_004002"
     CDS             complement(92..631)
                     /gene="scpB"
                     /locus_tag="PC472_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2729265.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTNE"
     gene            complement(633..1352)
                     /locus_tag="PC472_004003"
     CDS             complement(633..1352)
                     /locus_tag="PC472_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2729266.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1382..1978)
                     /locus_tag="PC472_004004"
     CDS             complement(1382..1978)
                     /locus_tag="PC472_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2729267.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
BASE COUNT          667 a          338 c          187 g          891 t
ORIGIN      
        1 ttatatttat acaaaaaaac acaacaactt agagcatttt aaatctttta aaatttaaaa
       61 ttacatacta aattattgtg attttatatt tttattcatt tgttaccact tcttgataat
      121 tttctatagg tggcaattct tcaagacttt ctatattcaa caactttaaa aattcatctg
      181 ttgttttata aattattggt ttccctatct tattcaatct accagcttct cttataagtt
      241 cattttcaaa caaagtttgt aataccttat cacattttac tcctcttata tcctctattt
      301 ctacctttgt tattggttgt ttgtaggcaa ttatagtaag agtttctaat gtagcttggc
      361 tcaaactttt ctttttcttt ggttcaataa tcttctttat atattctgca tagtctttat
      421 ttgtacacat ttggtactta ttctctaact tgattatttg aatacctcta ttttgttctc
      481 tgtactcctc aataagtaaa tttaacataa tctctatttc ttttgatgaa agttctttat
      541 ttattatata gtttaattct tttatactta ttggttcccc atatgcaaac ataacagatt
      601 ctataatata ctttatatct tctcttttca ttttaacact ccgaactttt tcttattaga
      661 atatcatcaa aaaatatatc ttgtactacg actatttctt tttcttttat aagctctaat
      721 atagataaaa aagtagctat tatttcatca ttttcatatg attttataat atttgtaaaa
      781 gtaacttcaa tcttctcttt tataatatct cttatatata atattttttc ttctacagat
      841 attattttct tccttactat tttatctagt ttctcatcat tagcattttc tatctgtgat
      901 gttttaactt taaatatgta tggaagtata ttttttattg cttcaattga tatatcttct
      961 aaatccacat tgtcatcaat aattatctct tctttatttc tataaaatct ctcattaaca
     1021 taagttatat tttcttttat atcttgtgat gctaccttaa actttctata ttcttctaac
     1081 ttttccataa gttctattct tgggtcttct tcttcatctt tttgcttata aagcagatat
     1141 ttagacttta tctctaaaag ttttgatgcc atagttataa attcactagt tatctctaaa
     1201 tccattttat ctagcatttt caaataattt agatactgct tagttatatc tattatagat
     1261 atatccttta tatctatttt atgctttgtt attaaatcat aaagtaaatc taatggtccc
     1321 tcatagactt gtaattgtat attatacttc atatcaatcc ctctttaatc tgtttattat
     1381 attaaacaaa taaatatact atttttagca acactgtata tattggtgat agtattattt
     1441 gatatatacc agtaaataaa gcaattaata aaattatata actcatactt tcaaatttat
     1501 acaaatattc attccattta tatggtacaa atgtagatat aactccccat ccatcaagtg
     1561 gaggtatcgg aagtaagtta aaagctgcaa atcccatact atataaaagc acatatccag
     1621 ctattgtatt tatagcgtac atatgcacat acttattgat aattacacac agtatagcag
     1681 tcaaaacatt acaaaaaacg cctgcaagtg aaactatcag gtttcctatc ttatagtttt
     1741 taaagttatt tggatttact ggtactggct ttgcccaccc tatcttaagg ataaacatag
     1801 ccaaaagacc tattgggtca acatgtctta caggatttaa tgtcattcta ccataagctt
     1861 tagctgtatc atctcccaat aagtgagctg aataagcatg gccaaactca tgtattgata
     1921 ttgccatagc tataccaact aaacttgcta ctatttctcc tatattcata cttaacatta
     1981 atgtacatct cctaaattaa atttttaata ttattataac aattaaatag cttataatca
     2041 aaattatttt acattataat aattttaact tctaggcata aaa
//
LOCUS       DAMJYC010000225         2045 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJYC010000225 DAMJYC010000000
VERSION     DAMJYC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.235"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            201..1340
                     /locus_tag="PC472_004005"
     CDS             201..1340
                     /locus_tag="PC472_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2729268.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1366..1554
                     /locus_tag="PC472_004006"
     CDS             1366..1554
                     /locus_tag="PC472_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729269.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          869 a          160 c          291 g          725 t
ORIGIN      
        1 ataactaatg tattataaat aatagttgta aacttcctgt gacatatatt ttaaatgctt
       61 attcttaaat ttttgcatta tttattaaaa tttattatgc tttaacgtta aattgagttt
      121 gataattata aaacaaataa tcaaaaatag tgtaaaaatt aatacttttt gttaatatta
      181 atagataaag ataatctaag gtggtgatta aactgacaat agaagaatac aaaaacaaat
      241 actttactgg aaataaaatc gtactttcta taaatagttt tgataataaa gaactatact
      301 atcatgaaac tgaaaatata ttaaatttaa taaatgaaat agataaatta acatcaagta
      361 agatatttaa aataatagaa gataaaaatg atttggcact agagtcttat tattctagtg
      421 taatagaagg tgctttttca aatagaaaga ttgcaaaatc tataattaga ggaaagatga
      481 aaccaagtaa taaaagtgaa tatatgattt acaataatca tagagcctta gaatatggat
      541 tagacaactt agataaatta tatagtcaca aatttatata tgatttgcat catatattgg
      601 gcgaaaactg cttggattca gaagaatatg agtatagaac tgaaaaagta tatgtatgtg
      661 attcaaaagg tgagattata catatagggc ttgaaccttt gaagatatat gactttatga
      721 gtaaattaat agattttatg gaaaatagca aagtaagtaa tttaattaaa agtgctataa
      781 ttcattttta ctttgtatat gttcatcctt ttagtgatgg taatggaaga acatcaagag
      841 ccttatcata tctatatttg attgataaag gatatgatac tttcaaggag ttttcaatat
      901 catatatgat atctaaaaac agaactaagt attataaggc tatacttgat gtagaaaaca
      961 aaggaaataa tttaactgta tttatagagt ttatgctcaa gtctataata caatctatta
     1021 atgagatgcg taatatgcat gatagaaaat ctttagaaag tatattaaaa gaggaattgt
     1081 ttgaaaatga cataactcta tcagcaacag aagaacatat attaaaatat atctgtagta
     1141 aagataacta ttctatgaca ttagaaaatt atataaaaaa gaataaaagt agatacttaa
     1201 aggctggaat aaaggaaatt gaattagttg accagttgat ggaagtgttt aataatcttg
     1261 aagagataga gattttgtct aaagaaaaaa atatatataa agttaatgat aagtacttaa
     1321 aaatgcttga tatagactag tttagataat ctggagaggg aatatatgaa taataagaaa
     1381 aaggcatttt tgatttctgg ggctataaac gtaatttcat taattattgt tttttcattg
     1441 tttcattata atattataga aaatagtaca caaaatatga taataactgt aatcttaatt
     1501 tttatttttg aaatgataaa aataaaaatc atagagaaat actataaact ataaacaaga
     1561 aatatctttt ctaagagcat attgtacaat atgctcttaa tttagttata tagatttaat
     1621 ataaataatg tattttttgt agatgattta tgtaagattt atgaccaatt tgtgtatgat
     1681 ttgtgttctt taatcatgac aatattatat tgtaaaaaat aaatttaata aaaaatgact
     1741 tggaatttat attctaggtc ttttttatat aaaaatttaa taagtttcaa atgttggtaa
     1801 ctttgacagt agaaaacgtc tattgagata acgaggattc taatttaggg ggagatatat
     1861 tagcacttaa agtagtatat gcaacagata taatctttac aaaagtaatt tgaattaaaa
     1921 tgtaaatata aagaataatt aaaaaaatat cgtaattatt tgtttttcat atggataaaa
     1981 ttggttattt aatatataat tacaagtata aataataaaa tttatttgta aaaagagtag
     2041 cactt
//
LOCUS       DAMJYC010000226         1964 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJYC010000226 DAMJYC010000000
VERSION     DAMJYC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.236"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            65..463
                     /locus_tag="PC472_004007"
     CDS             65..463
                     /locus_tag="PC472_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729270.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    899..1162
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1313..1864
                     /locus_tag="PC472_004008"
     CDS             1313..1864
                     /locus_tag="PC472_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2729271.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          825 a          186 c          295 g          658 t
ORIGIN      
        1 ttgtaatttt tagttatata tatattataa tgaactagtt aaaaattttt atgaggggga
       61 aaatatgaat aagaagaaaa aattgattgg gatagctata tcttttctac ttataatcag
      121 cattataaat ccattacaag tagaagcaga agctattatt gagtctagtg tatacatttc
      181 ttcaataaaa gagaattata catatgataa tactaaattt aaaactatta aatctaaaag
      241 tagacataaa agtaggaaat ataataaaag taaagtaaag ttgaaaaaac gaagtataag
      301 aactaaaaag tatggatatc aaactataaa taaatataaa agacaaaacg caagaagaca
      361 taatatccta acaaaattaa tattgtccat aatagtcata tttttggtta tagttttagt
      421 agtgctaata tttattaaaa gaaagaagaa aaaattttac taaaaataat taaaagaata
      481 tcattttttt aatcattggt atctatttaa taatatgatg taattaaaat ggagtaatat
      541 tctgtttcaa aggaggcata taaatacagg aatgagtatt aaatgatggt taattaagta
      601 aatttattga taaatgtact taattaactt tgaagaattt aattcctcat gaatcaaaat
      661 ataaaattac atcaacagtt attcacaaaa ttaatataag aagaattaga gccaaagagc
      721 agactattag accattaaag taattattca tagaattttg tatatgaaaa gaaatgtata
      781 gtataattta ctttttttat aaaaaataga ttacaaaata tattttttat aaaaaggtat
      841 tgacattttt tagaaaaaag aatatcatta ttttaacaac aaaattcaat acaataaatt
      901 aaatcctatg acaaggaaga gtagttaaat ttaggttcag tatagagagc tgaggatggt
      961 ggaatcttag caagaaaaaa tttgatgaat ggcccttaga ggaataaatt gaaatcatag
     1021 ttttgagagt agactttatc gtcagtcgca cgttatagcg aataaggtat gtaagtacct
     1081 gataatgaga tgtatgaaaa ttgaatttta tattaatttt tatataattt aggtggcaac
     1141 gcggataatc tccgtcctat tagtttagga cggagttttt tattttactg aaaatttaaa
     1201 agatagaagg tggtgagaaa gatgttatga atttcaaaag ctaaagatga tgatatagta
     1261 ggttggcaaa aaaataaatt gaaaattaaa aaaataggag gaagtacaaa aaatgaaaac
     1321 aaatacaaaa aaattaactt taaatgcaat acttttagcg atgggattat taatacatca
     1381 aatcacacca gctataggat taccaatgaa gccagatgta ccactagcaa tgttatttgt
     1441 aatattggtt ttaaatagag atgattataa aacttgtttg atagcgggta tagtaacagg
     1501 aatatttaca gcacttacat caagttttcc gggaggtcaa attcctaatg taatagataa
     1561 aactcttaca gcaaatatag tatttttatt aatgaatatt agttataata tgccttttat
     1621 aaaaaaatta gctaagaaaa cacaagatac tgtagtagtt gcaataataa tgccaatagg
     1681 aactcttgta agtggaacta tctttttatt agcagctcaa gctatagttg gtcttccagg
     1741 ggcttcattt acagctttat tcttagcagt agtattacca gctgttttaa taaatctagt
     1801 tgcaggaata tttttattca agattgttag tctatcaata agaagagtta gttatcaagc
     1861 ttaaacacga aataatattt tactttataa agcattataa taactaaata acataagaat
     1921 aaagtagatt ataactattt tattcttatg ttgtttaaag attt
//
LOCUS       DAMJYC010000227         1891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJYC010000227 DAMJYC010000000
VERSION     DAMJYC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.237"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            85..201
                     /gene="rrf"
                     /locus_tag="PC472_004009"
     rRNA            85..201
                     /gene="rrf"
                     /locus_tag="PC472_004009"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            208..282
                     /locus_tag="PC472_004010"
     tRNA            208..282
                     /locus_tag="PC472_004010"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asn,seq:gtt)
     gene            287..361
                     /locus_tag="PC472_004011"
     tRNA            287..361
                     /locus_tag="PC472_004011"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Glu,seq:ttc)
     gene            367..442
                     /locus_tag="PC472_004012"
     tRNA            367..442
                     /locus_tag="PC472_004012"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Val,seq:tac)
     gene            448..524
                     /locus_tag="PC472_004013"
     tRNA            448..524
                     /locus_tag="PC472_004013"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asp,seq:gtc)
     gene            535..609
                     /locus_tag="PC472_004014"
     tRNA            535..609
                     /locus_tag="PC472_004014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Thr,seq:tgt)
     gene            624..708
                     /locus_tag="PC472_004015"
     tRNA            624..708
                     /locus_tag="PC472_004015"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:658..660,aa:Tyr,seq:gta)
     gene            718..791
                     /locus_tag="PC472_004016"
     tRNA            718..791
                     /locus_tag="PC472_004016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Gly,seq:tcc)
     gene            802..878
                     /locus_tag="PC472_004017"
     tRNA            802..878
                     /locus_tag="PC472_004017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:836..838,aa:Arg,seq:tct)
     gene            888..963
                     /locus_tag="PC472_004018"
     tRNA            888..963
                     /locus_tag="PC472_004018"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Gln,seq:ttg)
     gene            975..1050
                     /locus_tag="PC472_004019"
     tRNA            975..1050
                     /locus_tag="PC472_004019"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Lys,seq:ttt)
     gene            1053..1141
                     /locus_tag="PC472_004020"
     tRNA            1053..1141
                     /locus_tag="PC472_004020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1087..1089,aa:Ser,seq:tga)
     gene            1159..1249
                     /locus_tag="PC472_004021"
     tRNA            1159..1249
                     /locus_tag="PC472_004021"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ser,seq:gct)
     gene            1258..1334
                     /locus_tag="PC472_004022"
     tRNA            1258..1334
                     /locus_tag="PC472_004022"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1292..1294,aa:Pro,seq:tgg)
     gene            1420..1495
                     /locus_tag="PC472_004023"
     tRNA            1420..1495
                     /locus_tag="PC472_004023"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Trp,seq:cca)
     gene            1556..1632
                     /locus_tag="PC472_004024"
     tRNA            1556..1632
                     /locus_tag="PC472_004024"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Pro,seq:tgg)
     gene            1639..1715
                     /locus_tag="PC472_004025"
     tRNA            1639..1715
                     /locus_tag="PC472_004025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1673..1675,aa:Ile,seq:gat)
     gene            1719..1794
                     /locus_tag="PC472_004026"
     tRNA            1719..1794
                     /locus_tag="PC472_004026"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Phe,seq:gaa)
     gene            1802..1878
                     /locus_tag="PC472_004027"
     tRNA            1802..1878
                     /locus_tag="PC472_004027"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1836..1838,aa:Met,seq:cat)
BASE COUNT          475 a          440 c          516 g          460 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaaatgtggt tataatagcg gagaagatac acctgtttcc
      121 attccgaaca cagaagttaa gttctctagc gctgatggta cttggtggga aactacctgg
      181 gagagtagga catagccacg tgaatgttcc aaggtagctc aacggtggag caaccggctg
      241 ttaaccggta ggctgtgggt tcgagcccca cccttggagc cattatggct cgttggtcaa
      301 gaggttaaga caccgccctt tcacggcggt aacaggggtt caattcccct acgagtcacc
      361 atttgtggga tcatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt
      421 tcgagccctg ttggtcccac catttgcggc ctggtagttc agttggttag aatgccagcc
      481 tgtcacgctg gaggtcgagg gttcgagccc cttccaggtc gccaaaataa aaatgctggt
      541 gtggctcaac ggtagagcag ctgacttgta atcagcaggt tgtaggttcg attcctatca
      601 ccagctccat gtaaaactaa tacggaggat ttcccgagcg gccaaagggg gcagactgta
      661 aatctgttgt catcgacttc ggtggttcga atccaccatc ctccaccaat taaatatgcg
      721 ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt cgatcccctt
      781 cacccgctcc aaagaaagcg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      841 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatatattgg ggattagcca
      901 agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct gcatccccag
      961 ccaaataaaa atatggtcta ttagctcagt cggtagagca cctgactttt aatcagggtg
     1021 tcccgcgttc gagtcgcgga tagatcacca atggagaggt gtccgagtgg tttaaggagc
     1081 tggtcttgaa aaccagtgac ttcgaaaggg gccgtgggtt cgaatcccac cctctccgcc
     1141 aaaaaaatga ctgaatttgg agaagtactc aagtggctca agaggatccc ctgctaaggg
     1201 attaggctgg gtaaccggtg cgagggttcg aatccctcct tctccgccat tcaaaaacga
     1261 ggtgtagcgc agtttggtag cgcacatggt ttgggaccat ggggccgggg gttcgagtcc
     1321 cttcacctcg accaacttaa gcttttagtt cagttggtta gagtgcccaa ctcatggcta
     1381 gtaattctag ggtttgagtc catgaaggtt attcttcata ggggtgtagc tcagttggta
     1441 gagcgtcggt ctccaaaacc gtgcgccggg ggttcgagtc cctccacccc tgccaaataa
     1501 aaaggacttg aaaaaagttg gattttagag tagtataaga tagtaaataa gatttcgagg
     1561 tgtagcgcag tttggtagcg cacatggttt gggaccatgg ggccgggggt tcgagtccct
     1621 tcacctcgac caagaaaagg gcctatagct caggtggtta gagcgcacgc ctgataagcg
     1681 tgaggtcgct ggttcgagtc cagctaggcc caccatttgc ccagatagct cagtcggtag
     1741 agcaggggac tgaaaatccc cgtgtcggtg gttcgattcc gcctctgggc accaggaata
     1801 cggcggtata gcttagttgg ctagagcgtt cggttcatac ccgaaaggtc ataggttcga
     1861 ctcctattac cgctaccatc taactatatc a
//
LOCUS       DAMJYC010000228         1882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJYC010000228 DAMJYC010000000
VERSION     DAMJYC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.238"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     repeat_region   39..530
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=171..199
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            complement(574..777)
                     /locus_tag="PC472_004028"
     CDS             complement(574..777)
                     /locus_tag="PC472_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729272.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            1021..1365
                     /locus_tag="PC472_004029"
                     /pseudo
     CDS             1021..1365
                     /locus_tag="PC472_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            1447..1800
                     /locus_tag="PC472_004030"
     CDS             1447..1800
                     /locus_tag="PC472_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729273.1"
                     /translation="MQFLVAKTFFKKAGVDIPLEIEEKEHFYDTKQIARKLGLYTMSN
                     KPAFQAVKQIIRKLDIKEDEMKTVLETNGSWTGTVTKYSNKVTEKVKEWLKENNNPIS
                     VKGDKKNYYILYKNN"
BASE COUNT          756 a          209 c          278 g          639 t
ORIGIN      
        1 gtatactcaa taaaaaattt atcaccatac tgttacacgt tttatattaa ctatgtggta
       61 tgtaaatgaa gcattattta ttttttcttc tattatatct atttgtttta tattaactat
      121 gtggtatgta aattagggaa ttaggaaatg gctattctcc ccctatatcc gttttagatt
      181 aactatatgg aatgtaaata tttgaacatt tgttacagca ttagagatat taattgcgtt
      241 ttagattaac tatatggaat gtaaatccaa ctgaataaac aataatttta tcaatttctt
      301 tagttttaga ttaactatat ggaatgtaaa tcatcatagt cacgtatatt ttctttaaaa
      361 gtcaaagtgt tttagattaa ctatatggaa tgtaaatttc gcaacttatg atggtgaaat
      421 gattacatta acaggtttta tattaactat gtggtatgta aataaagcaa aagataggaa
      481 tgataatttt aaaactccag acttttatat taacaatgtg gagaaaaact aaatagaaaa
      541 aagaagcact tacttaaata gtaggtgctt ttgttattct ttgctagata aagtaacaag
      601 ttcattaggt gtacattcaa gaacaagaca aagcttttcg aataaatcta attttataga
      661 acttgtttca ttgttagcta atttcatcat gttaggataa gctataccta cttctttagc
      721 taaccaatat ctagttcgac ctttggattc aagtaattta tcaatgttaa aatacatttt
      781 ttcacctctt aagtatatta tacaacaatt atataatgtt tgcaatatat aatatgcaaa
      841 caatataatt atataatgtt gacattatat aatgtgtacg atataatata cttaaggaat
      901 aaaaaataag agccactcgc ccctaccaag tttgagtaac tcttattgac atatactaca
      961 tatgcattaa ttatagtata tgtcattcct taaaaaaaat caattaagga gtgtgttttt
     1021 atgaacaatt tagtactaat taacaatcaa gaagtgcaag ttaaggaatt taataatcaa
     1081 agagtagtta catttaaaga aattgaccaa gtacatgaaa gaacagaagg tacagcaaag
     1141 agaaatttct cagagaatag aaaacatttt atagagaacg aagatttctt ttttgtaaaa
     1201 gcaagtgatt ttgaaaagta cggaaatcgt actttagaaa ttcctaatcg tggattaact
     1261 ttaataactg aaacaggcta tttgatgtta gtaaaatctt ttacagatga cttagcatgg
     1321 aaagtacaaa gacaattagt aaatagttat tttagagtta aagaagaaat aaaaacatta
     1381 gaagcagtaa atgagtcaat taaattaata actccaattt ttgaagattt aaaaattgat
     1441 aaaagtatgc aatttcttgt tgctaaaaca tttttcaaaa aagcaggagt tgatattcca
     1501 cttgaaattg aagaaaagga acatttttat gatacaaaac aaattgcaag aaagttagga
     1561 ctttacacaa tgtctaataa accagcattt caagcagtta aacaaattat aagaaagctt
     1621 gatattaaag aagatgaaat gaaaacagta ctagaaacca acggttcttg gactggtact
     1681 gtaaccaaat attctaataa ggttacagaa aaagtaaaag agtggttgaa agaaaataat
     1741 aatcccattt ctgttaaagg agacaagaaa aactattata tattgtataa aaataattag
     1801 attttagttt tgagtttgag ggggattaat aaaatgcgtg aaagtttact taatgaatat
     1861 aatttaaaaa ctgatgaaga tg
//
LOCUS       DAMJYC010000229         1842 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJYC010000229 DAMJYC010000000
VERSION     DAMJYC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.239"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            127..1176
                     /locus_tag="PC472_004031"
     CDS             127..1176
                     /locus_tag="PC472_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2729274.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1326..1673)
                     /locus_tag="PC472_004032"
     CDS             complement(1326..1673)
                     /locus_tag="PC472_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2729275.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          687 a          246 c          284 g          625 t
ORIGIN      
        1 acataaaact gaaaattatt attaagtttt tgtagaaata ttataagtat agatacattt
       61 ttgtaacaac tacaataaat atttaatact atatactatt cgtatgtctt atgggaggta
      121 tttattatgt ttaaaaaaat agtcgcaata gcaagctcta tcattgtaag tacaatatgt
      181 ctaactggat gtgattttaa ctctaatgac aaaaaagatg aatcttcttc gtctaaaaat
      241 ctggataaag taactatagc agaagttact cactctgttt tttatgctcc acagtatgct
      301 gctattacga aaggattctt tgaagatgaa ggtataaaga ttgaccttat aaatacccaa
      361 ggtgcagata aaactatggc agctttgatt tcaggtgaag caaacgtagg tttaatgggt
      421 cctgaagcat ctatatatgt atataatcaa aacaaaaacg attatgcagt gaactttgct
      481 caattgacaa aaagagatgg tagttttcta gttgctagga aaaaaatgcc taatttctct
      541 tacaatgatt taaaaggaac cgaaatatta ggtggtagaa aaggtggagt tccactaatg
      601 acttttgaat atgttctaaa agaaaaaggt cttactattg gagaaaatct aaaagcaggc
      661 aatgttaatg taagaactga tgtacaattt ggtgtaatgg ctggagcttt tgcaggtgga
      721 gagggtgact ttacaacagc atttgaacct acagcaacag caatggaaaa agaaggtagt
      781 ggatatatag ttacatctat aggaaaagat tctggagaaa taccttttac agcatactct
      841 actacgagat catacatgaa aaacaataaa gatttaattc aaagatttac aaatgctgtt
      901 tataaaggtc aattatggat tcaacaagca tctagcaaag aagtagctaa ggctatgcaa
      961 cctttctttg aagatatatc tttagaagat ttaacaacag ttgtagatag atataaatca
     1021 atagatgcat ggtctcaaaa tcctgtatta aaagaggaag gtttgaagaa attgatgact
     1081 gttatgaaag aagctaaaga actagataag gaagcacctt ataaagatat cgtaaatact
     1141 gagtttgcaa acaatgctat aaaaaatatt aaataaaata tattagtttt agctagatat
     1201 tttcttgttt ttataatgaa aattaacaaa aatataaaaa gagcctttta aaagactctt
     1261 tttatatttg agattaaagt ttatataata aaatagatat taaagtacta actgtactta
     1321 tatatctatc ctctgtattc tgctccagca ttaatttttg taatttgaac ggctaccatt
     1381 tcttcattgc ttccattagt aatttcatgc cactctcctg ttggtacaaa tacagtatcg
     1441 ccgtttttta catcacattc atgctcacca agtttcattt taccttctcc ttttaggaac
     1501 acaaatattt gttcaccatt agggtgtcta tgagcattaa gaacttgatt aggtttaaaa
     1561 taataaactt ctgaagttat atcgttacca gcaaatactg gtatttttgg aactgctgta
     1621 tcattatatt gagctaagct tccgtctaat agattctttt tattttcatg catactagaa
     1681 atacctcttt tctatattat agatttacta ttattatacc aatataagaa aatagtattc
     1741 tcactttaca caataggaat aaaattcttt tatttttaaa tataagtata atactatttt
     1801 tatagttaga aaaatacaat ccttataaga tataaaaaat ta
//
LOCUS       DAMJYC010000230         1836 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJYC010000230 DAMJYC010000000
VERSION     DAMJYC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.240"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            74..1786
                     /gene="ptsP"
                     /locus_tag="PC472_004033"
     CDS             74..1786
                     /gene="ptsP"
                     /locus_tag="PC472_004033"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2729276.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT          709 a          197 c          367 g          563 t
ORIGIN      
        1 tataaaaatg taaattattt atataaattt ataaagttta tgtgctagat gatatttttt
       61 aggaggtagg gttatggctt acaaaggaat aggtgcttct cctggtgttg ctttagggaa
      121 agcgttagta gttgaacata gtgaattagt aattgaaaaa aaatctatag ataatgttga
      181 agctgaaatt gctaaattag aagatgcagt tgcagtttct aaagaagaat tagtaaaagt
      241 taaggaaaag gcttctgaag aattaggagc agaggaagca gaaatatttg aagcacattt
      301 attagtttta gaagacccag agttaattgg ttctgcaata gataaaatta aaactgaaag
      361 tgtaaatgca gagtatgctt taaatgaaat aaaagagatg tttgttagca tgtttgagtc
      421 tatggacaat gagtacatga aagaaagagc ggcagatata aaagatgtta caaatagaat
      481 acttagacat atattaggaa ttaaagttgt tgatttatct gctttaagcg aagaagttgt
      541 attaatagca catgatttaa ctccatcaga cacagcaact atgaataaaa aaatggtttt
      601 aggattttta actgatatag gtggaagaac ttctcatact gctatcatgt ctagaacttt
      661 agaaatagct gcaatcgttg gattaaatga tataacttca aaagttaagg atggagattt
      721 tgtggttttc aatggagaca caggtgaagt aatcgtgaat cctgatgaag agactataaa
      781 taaatatact gaattaaaag ctaagtatga agatgagaga aaagcattac agctattaaa
      841 aggaaaacca tctgtaactt tagacggaaa acatgtggaa ttagctggca acataggaac
      901 acctaacgat atagaaggtc taataaagaa tgatgctgaa ggtgttggtc tttatagaac
      961 agaattttta tacatggata gagattcttt cccaacagaa gaaatacaat atgaagcata
     1021 caaagctgta ttagaaggaa tggatggaaa gcctatagtt ataagaacat tggatatagg
     1081 tggagataaa gaattaagtt atttaagtat ggaacctgaa atgaatccat tcttaggtta
     1141 cagagcaata agactttgcc tagatagaaa agatatattt aagactcaat taagagcttt
     1201 atatagagct agtgttcatg gaagattaag aatcatgttc ccaatgattt catctttaga
     1261 agagttgtta caagctaaag aagttgtaaa agaagtttta gcagaacttg attctgaagg
     1321 tgtggcttat gctaaagatg tagaaattgg tatgatgata gaagttccat ctgctgctgt
     1381 aatatctgat gtattggcta aacacgtaga tttcttctct ataggaacta atgatttaat
     1441 tcaatacact tgtgcagttg atagaatgaa tcaaaagata agttatcttt acaatcaatt
     1501 taatccagct gttttaagac ttataaagac tgttatagac aatgcacata aagaaggaaa
     1561 atgggcaggt atgtgtggag aatctgctgg agaccaaaaa atgataccaa tacttttagg
     1621 tatgggatta gacgagttct ctatgtctcc aatatcaata cttcctgcta gaaagttaat
     1681 aacttcagtt aaagagtcag atatgaaaaa attagctgat gatgtactta atatgggtac
     1741 tgctgaagaa ataaaaagct atatagaaaa gacttttaat atataattaa tgttatatag
     1801 ttactaaaat tcatttttct aaataacaaa attata
//
LOCUS       DAMJYC010000231         1813 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJYC010000231 DAMJYC010000000
VERSION     DAMJYC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.241"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            84..653
                     /gene="mocA"
                     /locus_tag="PC472_004034"
     CDS             84..653
                     /gene="mocA"
                     /locus_tag="PC472_004034"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2729277.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            650..1669
                     /locus_tag="PC472_004035"
     CDS             650..1669
                     /locus_tag="PC472_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2729278.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          728 a          161 c          347 g          577 t
ORIGIN      
        1 attatatata tagataatat caatagagat attataaaaa tcatagaact atcaatatat
       61 tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca ggctttgcca
      121 gaagaatggg agcaaataaa cttttattaa attataaagg ctgttctata atagaacatg
      181 tatttagaga agttagcaag attgattttc atcaagttgt agtagtaagt caatatgaag
      241 aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa aatgcaaata
      301 taggtcaaag tgaatctata aaattaggag ttttaaatag ctcaaaatgt gatggttata
      361 tgttctttgt aggagaccag ccatttattg ataacttata tatagataaa attataaata
      421 cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg tgtggaaatc
      481 cagtcatatt cccatatagt aagaaagaag aattgttggc acttaaagaa gatgaaaaag
      541 gtaaaaccat tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct aaagaaatgt
      601 tatttgatat cgatacaaaa atggattatg agagaattgg gggtaaatta tgaagctaat
      661 aaaaactata gatgctgttg gacaagtttt atgtcacgat ataactcaaa taatacctgg
      721 agaatttaaa ggaagaaaat ttaaaaaggg ccacatagta aaagaagaag atattccagt
      781 gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag gaatggttca
      841 tgaaaatgaa ggagcattgt ttttaaaaga actaactgct ggagaaaatt tagattttag
      901 tgaaataaaa gaagggaaaa tagattttat agcagcatgt gatggtctat taaagataga
      961 tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta ctcttcataa
     1021 taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac ctttagttat
     1081 agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa tagtaaatgt
     1141 agtacctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag tgttttatag
     1201 cagaattgtt gataaatttg gaccagtaat agaagaaaaa gtaaaaggct ttggttgtga
     1261 agttatagga cagactatat gcccagatga taaagagatt ataaagagtg caataaaaga
     1321 atttataagt caaggagcag aacttatctg ttgtactggt gggatgagtg tagacccaga
     1381 tgatgttacg ccaacagcta taaaagaaac tggagctgat ttagtgacat atggttcacc
     1441 aatattacca ggagctatgt ttttacttgc ttattatgga gaagttccaa ttatgggtat
     1501 accaggatgc gcaatgtatc acaaaactac agtatttgat atagttttat ctagggtttt
     1561 aattgatgaa aagcttgata agtatgatat agctagatat ggtcatggtg gcctttgtat
     1621 gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa gttattaaaa
     1681 aagagtattt acaaattaag tactcttttt aaatatgaaa caaaacagct agaatgatgt
     1741 tttaaaaaaa gcatatttat ttagaaatta taataactta ttttcaatgt gtaaaagaat
     1801 gaataaagtt gca
//
LOCUS       DAMJYC010000232         1784 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJYC010000232 DAMJYC010000000
VERSION     DAMJYC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.242"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..470)
                     /locus_tag="PC472_004036"
     CDS             complement(<1..470)
                     /locus_tag="PC472_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729279.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENEL"
     gene            complement(564..1289)
                     /locus_tag="PC472_004037"
     CDS             complement(564..1289)
                     /locus_tag="PC472_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDF2729280.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
     gene            complement(1337..1735)
                     /locus_tag="PC472_004038"
     CDS             complement(1337..1735)
                     /locus_tag="PC472_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729281.1"
                     /translation="MQKDVWLYSWDDECFGSDEYESKEEAIQGAKEELRRFGEVSRLV
                     YVGQKEEVNIPNLNVEDALEHVQDRIDNEFMGYGEDWFEKIRVEDILILENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
BASE COUNT          508 a          359 c          146 g          771 t
ORIGIN      
        1 agttcatttt ccttaattct tggataaagc atatccataa ttttatacac taatctttcc
       61 ctcacacatc tacatgtttt tctatctgag ttcatctcta aggatatata aaccatacta
      121 tttttcattc tgctattata aaacagttta aaaaaatgtt cttctcttat atctaagcat
      181 gtaagtgcat tttctatttt cttcttttca atttccttat cttttttcag ttttttcaat
      241 ctagtaatat ctctttcttt ttttataatc tcattctcca cacttgaatt aaaagcatat
      301 gttggactta ctttttcatc atatccaaca gccttacacc caaatatctc attttctcta
      361 ctttctatat ctaattcaag atttttaatt tctgcactta aaaatttata atgatgtagt
      421 ctaccttcta cttttttaaa tagttctttt ttattgatat tattatccat acttccacac
      481 tcctgtttat gttataataa tcttggataa aagttttata tttttgacga ctggagtgtg
      541 aaagcactcc ttttttcttt ttattaacag aaattatctt tttcaaagaa actaatctga
      601 tttgtctttc tttcagattt tataattcta attgattcat ctattaagtt taatgaatta
      661 agtaatacat ctttcgggat atcctcccat ttgtcagcac ctaataccaa tagagttctt
      721 ttcttaacta attcaaattc ttcattaact tttgatatac ctagtctttc ttttatataa
      781 gaagatatat catatttagt tttagaggtt ggtctataat attctgaaca ctctttttta
      841 agttgctcta tttggatatt atgtttaact tccatcttaa gtaatgattc attcacaata
      901 gtattaattt gactaagctg tgaatttgat aaggttctat tgagcaactt ttctaatctt
      961 ataaaatatc ttcttatttc tctcccttta ttattgtttt gtaccatagc aagttctttt
     1021 gccacatcaa gcttcaatac atattctttt gaaggtctcc caccagttga gtttttcata
     1081 tttttgtgaa aaactgaata atcctcattt tccttaaatc catattgttt aattctatct
     1141 tcaatccagt ctataaactg tctcttaact tctaagttat tatgtagttc tcttgcaaaa
     1201 actattttct ctcctgtatc agtttcataa actgtaacta aatcatctgc tacaactctt
     1261 aaattttcat ttgtcataat ctcattcata tttatagtcc tccttaaaac caactggctc
     1321 tccatctatt acaaaattaa ctttcatttg ctacctcatt tagttctatt tcttccgtat
     1381 ctctaacaac aaaccaatat ggtttatatc caaattcatc tatccatttt ttaaatactt
     1441 tgcttattct attctctagt attaatatat cttcaacacg tattttttca aaccaatctt
     1501 ctccataccc cataaattca ttgtcaattc tatcttgaac atgttctaaa gcatcctcta
     1561 catttagatt aggtatatta acttcttctt tttgcccaac gtaaaccaat cgactgacct
     1621 ccccaaacct tctaagttct tccttagctc cttgaatagc ttcctcttta ctttcgtatt
     1681 catcacttcc aaagcactca tcatcccagc tatataacca aacatctttt tgcatattaa
     1741 tacctccact atttattttt cttattagcc ttcttcctac attc
//
LOCUS       DAMJYC010000233         1745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJYC010000233 DAMJYC010000000
VERSION     DAMJYC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.243"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..154)
                     /locus_tag="PC472_004039"
     CDS             complement(<1..154)
                     /locus_tag="PC472_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2729282.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSILDVAVLIGFNS
                     QNYYSII"
     gene            459..650
                     /locus_tag="PC472_004040"
     CDS             459..650
                     /locus_tag="PC472_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729283.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(739..1551)
                     /locus_tag="PC472_004041"
     CDS             complement(739..1551)
                     /locus_tag="PC472_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2729284.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
BASE COUNT          653 a          243 c          198 g          651 t
ORIGIN      
        1 aaattatact atagtaattt tgactattga atccaattag tacagcaacg tctaaaatag
       61 acaagtcttt ttttaatata tactttttgc ttttttcaac tctaacttta tttaaaaaat
      121 tagtaaaagt atatccactt tttttcttaa acaaagaaaa aaacaacttt tattaagtta
      181 taattcatta catatattac ttactgttat atcttctgag taatttttgt gaatataatc
      241 tatagttttc tttaagtttt ctttacataa aactaagagt tggtctatgt ttcattttat
      301 ccaggcatat ttattaaaaa atcaatctct atttgttaaa ataatttatg aatgatgtta
      361 gaaactgtaa tatattggat ataatataat aaacagataa actctgtatt aagaggatat
      421 atagtttatc ttaattatag gggaagtgag gatatattat ggaaagatta atggaactca
      481 tagaaagaaa atttttaaag gatgtagaac ttttagaatt agaagacata gaaaatgtaa
      541 tagaatgcaa aatgacacag ccatataatg ctcattataa tgaatactta gtaaaagtaa
      601 aaaatggaga tgaagtagaa gaatattttg tgtatttaaa acatagataa acataaaaga
      661 ggtttatatt tatttgtatg attataaaat aaatacaagc ctcttttttt gataaatata
      721 aaattataaa atttttattt atttaatttt ttcaacaaca ttagtcaata ttgaatctaa
      781 atcatcacta atttctgtaa ctccattatg agtaatatct ataatttctt tagtcatagg
      841 aagttctcct aaaagttcta agtctaaatc atctaaaaac ttttcagtag attctccttc
      901 aaataacttg atttttttag aacaatcagg acattggata taactcatat tttcaataac
      961 tccaaggaca tttatgttca tttttttagc catattaaca gcttttgata caatcataga
     1021 taccaaatct tgaggtactg aaaccataac tattccactt ataggaatag attgcataac
     1081 tgttaaggca acatctccag taccaggagg catatctatt aataaatagt ctaattcttc
     1141 ccaaagtaca tcagtataaa attgctttac aacccctcca agtaaaggtc ctctccaaac
     1201 aacaggttca ttttcatcat ctatcataag gtttatagac attactttta tgttatttga
     1261 gttttctaca gggtaaatat aagagccatc agaataagct tttacatttt ttacacccat
     1321 aagtctagga atacttggtc cagttatatc tgagtctaat ataccaacct tatatcccat
     1381 tttgtttagc ttattagcaa gtaaagcagt tacagttgat tttccaactc cacccttccc
     1441 actcataaca ccaattattt ttccaaattt attgttaggg ttattttcaa tagaacaatt
     1501 tgattgacta ttacaatttc cctttgatgg acatgaatta caatctgcca tatctattac
     1561 ctccaaactt taatatttat tagattattt gtaacctaca tatactattt tagtacaatt
     1621 gaaccataaa gtcattattt aaaagcaact tagaagattt tctattgatt tattcataga
     1681 ctatagcaaa aagaatacaa taaaaaaatt ataacattct tgaaaaagtg attatttcaa
     1741 tttag
//
LOCUS       DAMJYC010000234         1710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJYC010000234 DAMJYC010000000
VERSION     DAMJYC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.244"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            89..1417
                     /locus_tag="PC472_004042"
     CDS             89..1417
                     /locus_tag="PC472_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2729285.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          577 a          202 c          319 g          612 t
ORIGIN      
        1 aataaagcta tttttctata tattggcata atatatgctt atttaactat gaaattaaaa
       61 ttaaatagga ttaatgagga ggaaaaaaat gggaagtaat gaactaaaaa agactcttgg
      121 cgtatcagca gcattatcaa ctgttgttgg ttccgttata ggagcgggcg tattttttaa
      181 accacaagca gtctatacat taactggagg agctccaggt cttggtatat tggcatggtt
      241 aatagctgga atcataacta taacagctgg tcttacagct gcagaggtat cagttgcgat
      301 accaaaaact ggtggaatga tggtatatat aaaagaaatt tatggagaaa aattaggttt
      361 tttaactgga tggatgcaaa ttgtattatt ttatccaggg atgatggcag cattaggtgt
      421 tatatttgga gaacaagcat cagcactaat tggtagtcca tcacttttac ttccaatagc
      481 aattggaatt atagtaatag ttgcagggct taatatgtta ggttcaaaaa ctggaggagt
      541 catacaaact gtttctacaa tttgtaagtt aataccactt attttaataa tggttgttgg
      601 atttattaaa ggtggtggaa ataatccaat acttacacct atggttggtg aaggattaag
      661 cctgggtagt gtactaggtc aagttttaat tgctatatta tttgcttttg atggatggat
      721 gaatgttggt actttagctg gagaaatgaa gaatcctgga aaagaccttc caaaagctat
      781 aataggtgga ttgtcagttg ttatggctgt ttattttata ataaatttag cgtatctttg
      841 ggtattacca gctaatgaat tagcaaatta tgcttctcct gcatcagcag ttgctgaagt
      901 tatatttggt tcaatgggtg gaaaaataat atctgtaggt atattaattt cagtgtttgg
      961 agcattaaat ggatttttac taactggttc aagggtggca tatactttag ctacagataa
     1021 gactttacca aagtatggta tattttctaa attaaattct gctcaagttc cagcaaatgc
     1081 aatagcatta gtatctgtta ttgcatctat atatgctttg tcaggtcaat ttaatttatt
     1141 aactgactta gctgtatttg ctacttggat attctatgta ttaactttta taggggttat
     1201 gaaacttaga aagactcatc caaatatacc aagagagtat aaagttccat tatatcctat
     1261 tgttcctata atagctatag caagtggtat atttgttgta gttaatcaat tgtgttttgc
     1321 aggtatgaaa actactatga tatctatagg aggtcttgta ataacagcta taggcttacc
     1381 agtgtatgct tatatgacta gaggagtaaa aagataaacc aagtagttta caaatgcttc
     1441 ataattaaaa taaaagagac tgtctcaaat agagataagt ctctttttta ttgttaaaat
     1501 ggtaaagata tgtttgattt tacaaaaaaa gagcgttcca tgaactaaaa agtttatttt
     1561 aaggtattca ttttattttt agttttttga ttataaaatt aaacatctaa aaaattattt
     1621 attttaaaat actttatttt aaagtgtttt ttatcaaact aagtggtatc tgtacaacaa
     1681 atattataca tagcttttat gaaaattaaa
//
LOCUS       DAMJYC010000235         1650 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJYC010000235 DAMJYC010000000
VERSION     DAMJYC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.245"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            295..1566
                     /gene="serS"
                     /locus_tag="PC472_004043"
     CDS             295..1566
                     /gene="serS"
                     /locus_tag="PC472_004043"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2729286.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          696 a          170 c          299 g          485 t
ORIGIN      
        1 aaaagttaat aactatacaa gattgataaa gaaaaagtca aaatagataa ttgattagca
       61 aaatagatat aattataatg aagttaaatc tagcaatatt ctcctaaaat taaatttcat
      121 ttatttaatt aaatataatt tgacaatggg caaaaaagtg agtaaaatat aaataaaata
      181 aaaataacag gttcggataa agaaaaaggt ttcttaacct caagcataaa gtatattatc
      241 taagttattt gagattaaga gataaatcca atcttaggta ctgaggagga aaaaatgctt
      301 gatataaaaa gaataagaga aaatttagat tgtataaaaa aagcaatgga aagaaggggc
      361 gaaaaagact ttaaccttga tgaagtagtt aagttagatg atgaaagaag aaagattctt
      421 caagaagttg aagttatgaa aaatgaatta aatacagcat ctaaaaatat accaaatctt
      481 ataaaagaag gtaaagatgt agaaaatgaa aagattaaac taaaagagtt atctgataaa
      541 attaaggtaa tagaccaaaa tcttaaagaa gtagaagata agatggaata cctattaatg
      601 agaataccaa atgtaccaca tccagaagta ccacaaggtg aaactgatga agataatgtt
      661 gaagtaagaa cttggggaaa gactactacc tttgattttg aatctaaggc tcattgggaa
      721 attggaacag aattaggtat acttgatttt gaaacagcta gtaaaataac tggttcaaga
      781 tttactcttt acaaaggatt aggtgctaga ttagagagag cattattaaa tttttactta
      841 gacacgaata caaaagtaaa tggatataca gaagttattc caccatttat ggcaaataga
      901 aatagttttt taggtacagg acagttacct aagtttgaag aagatatgtt taaaatagaa
      961 ggtctggact attttatgat tccaacatca gaagttcctt taactaatat acatgctaat
     1021 gaaatactta aatttgaaca attaccgata aattatacag cttatactcc atgttttagg
     1081 tcagaagctg gttctgctgg aagagataca agaggtttag taagacagca tcagtttaac
     1141 aaagtagaaa tggttaaaat agttgcacca gaagaatctt acaatgagtt agaaaagctt
     1201 actaacaatg cggaaactat gctacaatta ttgaatctgc cttatagggt agtaaagata
     1261 tgtacaggtg atttaggatt tacggcttct tttaaatatg atgtagaagt ttggatgcca
     1321 agttacaata ggtatgttga gatttcatct tgttcaaact gcgaagattt ccaagctaga
     1381 agagcaggaa taagatttaa aagagataaa gattctaaag ctgaatatgt acacacatta
     1441 aatggctctg ggttagctat tggaagaagt gttgcagcta tattagaaaa ttatcaacaa
     1501 gaagatggtt ctgtggttgt tcctgaagtt ttaagaccat atatgggagt ttctgttatt
     1561 aaataagagt taaattatgt atttttaagc aatacaataa tatagaggta ataaaaaata
     1621 attaatataa aagtgataca ataatataga
//
LOCUS       DAMJYC010000236         1517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJYC010000236 DAMJYC010000000
VERSION     DAMJYC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.246"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            42..1478
                     /locus_tag="PC472_004044"
     CDS             42..1478
                     /locus_tag="PC472_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2729287.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          620 a          160 c          262 g          475 t
ORIGIN      
        1 tttatatatt aaaaatttat tatgaggggg acaattttat tatgaaattc tataaaagaa
       61 tattaacatt gactttaagt atttcttttg tatttgcaaa tattcaatta gtagatgcaa
      121 tatcatctgt agaaaaaata caaggaaaaa ataagtatga aatcgcaggt aaaatagcag
      181 ataaaaatgc atataaaact gctatattaa ttaatactag taactctatt gcagatggat
      241 taagtgcaag tggactagca ggagctttaa atgcccctat actattaact gaaaaaaata
      301 ctataccaac tgagacatct gcaagattaa aaaatgtatc aaaagtttat ataattggag
      361 gaacatattc tattagtaca tctgtcgaaa attcattaaa atctaagaaa atgaaagttg
      421 taagaattaa gggaaatgac agaataaaaa caagttataa tgttgcaaaa gaaataaatt
      481 caataaaaaa agtcaatact gttatgctaa ccaatgcata taaaggagaa gcagatgcta
      541 taagtattgc atctgtagca gcaagagata aagcaccaat aattcttact aatggacaaa
      601 gtataccttt ttcaactagt ggtttaaagt cttatgtaat aggaggaaca gcttcaatga
      661 gtaccacttt agtaaacaat actaaatcta ctagactagg ggggagtact cgttttgaaa
      721 ctaataaagc aataataaat aagttttata aggatgctag agagttttat atagctggag
      781 cttatgaatt aacaaatgca cttgtaggat cttctttatc aaaacatggt ccaatggttt
      841 tagttaatga tggtagcaat aaaagcattt taaaaaatgc taagaagata acttctatag
      901 gatatataga ttcaaatata gtacaacagt gtttaaatat aacaaatgga attggagata
      961 ttaatactgg ggttgtaaaa aatgttaaac ctactacaaa gactataaaa gatggaatgt
     1021 ataaggttgg aaaagatatc tcagcaggtg aatacttaat aacttcaaac tcaggaagtt
     1081 atgcttctta ttatgaagtt acaagtgata gtacaggtaa tgctgatagt atactttcaa
     1141 atgatatttt tagtgggact agatatatca ccttaaagaa tggtcaatat attaaaattg
     1201 aggattcaac aatgacatta gcaaagtatg ctaaagccca gaaggccaag aatggtaagt
     1261 ttggaaatgg tatgtataaa attggattag aaattcctgc tggagaatat attataatgt
     1321 caaattcttc tgatgcatat tatgaagtta gaaatgatag cttaggtaat gcagagggaa
     1381 tagttactaa tgatactttt tcaggaagaa gatatataac ggttgaggaa ggtcaatatt
     1441 taatattaaa tgattgttat ttaattgaaa atgaatagat gtaattgtat ttaatagtaa
     1501 atcatatatt ttattta
//
LOCUS       DAMJYC010000237         1501 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJYC010000237 DAMJYC010000000
VERSION     DAMJYC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.247"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            13..1293
                     /locus_tag="PC472_004045"
     CDS             13..1293
                     /locus_tag="PC472_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2729288.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          632 a          194 c          172 g          503 t
ORIGIN      
        1 aaggagtatt ttatgaacat acttggatta atagttgaat ataacccatt tcataatgga
       61 cacatatatc atttatctaa atctatagaa aaaacaaatg caacacatac tgtagcaata
      121 atgagtggaa attttctaca aagaggcgaa ccagctttat ttgataaata cactagagca
      181 aaaattgctg tcgaaaatgg tgtagactta gttatagaat taccaacttt atttgcttgt
      241 caaagtgctg aaattttttc tcatggtgct ataacaattt taaattcact aaactgcata
      301 aattctatat gttttggtag tgaagaagga aatacagaaa ttctttataa tatagctgaa
      361 attttagcaa atgaaccaaa tgaatttaag ttacttttaa aaaaatattt agatgatggt
      421 ctgttatttc caacagctag agctaatgca ctttttgatt atatacatca agacaaaaaa
      481 aataatttta tacaaaatta ttcaaaggaa caattatcaa aaacattaaa ttcatcaaac
      541 aacatactgg gtatagaata cataaaaaac ctcatccatt taaacagtag tataaaacca
      601 tttactatct ccagggtcca atcagaatat aattccgaag aaattgagag tgatatttgt
      661 tctgctactg ctataagaac ttcacttaaa gaaaatacag acctttttca tttaaaaaat
      721 gttattccag aaaaaactta taacatccta aatgaaaaaa tagataatgg ttttttacct
      781 atgtttgatg acttcttctt tgatatttta aaaacaatta tacttagaga tatggacact
      841 ttaaaagagt attttgaggt aaatgaaggc atagaaaaca gaatctataa agatatcttt
      901 acatctgaat ttctacatga actacaatta gcaattaaat ccaagcgata cactttaacc
      961 aaaatcaaac gaactctaaa caatatattg ctaggtatta aaaaaagcga catgcaactt
     1021 ataaaaaaca tagatactat accttatata agaatacttg cttttaatga taaaggtaga
     1081 gaaattctaa aagagataaa aaataaatct gaaataaaaa taataaacaa gttttcaaat
     1141 atatcatttt cattagatga tacgattttt aaaacactca taagttatga tataaaagct
     1201 acaaatatat ataattcaat atattataaa aacaataaag atttagtaaa aggccctatg
     1261 gatttttata ctcatccaat atatgtaaag tagtttttta aaataactat atttataaaa
     1321 atcattttat taactcttta ttatataaga tacaatagtc ttactttatt tatgtacaca
     1381 tgcttttagg atgacaacat caacttaaat attatgttac tttctaaatt taagtaaata
     1441 ttgccatcct aaaaatttta caatttagct tcagatttaa aagtaaagtt taaaatatta
     1501 t
//
LOCUS       DAMJYC010000238         1486 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJYC010000238 DAMJYC010000000
VERSION     DAMJYC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.248"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            165..410
                     /locus_tag="PC472_004046"
     CDS             165..410
                     /locus_tag="PC472_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2729289.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(628..897)
                     /locus_tag="PC472_004047"
     CDS             complement(628..897)
                     /locus_tag="PC472_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2729290.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          598 a          216 c          169 g          503 t
ORIGIN      
        1 gtaattaagt gactcagtga tttagaggtg gtaaaatata tatatatgaa agagcataaa
       61 taaattgata taataaaaaa ttaaatatta aatattaact ataataaaaa aatatctggt
      121 aataggtata atataaaaag aatataccag aaagaaggtg aagtatggga gaactacaaa
      181 aaattccagg ggtagggaaa gcaacagaaa aatctttaat catgttagga tatacaacta
      241 ttaaatcact taaagatgca aacccagcac aaatgtatga aaaggaatgt ttaatgcgtg
      301 gacagcacat agacagatgt cagttatatg tatacagatg tgcagtatat tttgcatcaa
      361 ctgaaaatcc agaaccagaa aaattaaagt ggtggtattg gaaagattaa aaaatcaaaa
      421 aagttagaca taaaaaagag ttgaatatcc gttttaaata ataaaaacta atactcaact
      481 cttttttagt cgtaaatttt atgtaaatct atttgtatgg ctaaatatac ccatcaataa
      541 atgtataaat atactaatca acaaatgact aaacatacta cctaacaaag gattaaataa
      601 attccttaac aaagcattct aaaacaatta attagaaact gtttcttcaa gccattttct
      661 acattgctca atatttacca tagggtcaaa accttggtct tttcccttat ttaaaagcat
      721 atcacaaggg aaagtatcac attcaccaca atgattaagt tccttacttt cacagcaagt
      781 tttaacctta cattctccac cccaaaatgg tttttccata gttgggcaac cagtgcaatt
      841 tacttgttct ttttttgtac aactgtcaca acagacacca catcttgatt caaacatatt
      901 caaacatcct ttccattatt taaaaatatt ataaaatgat tttagatgaa tacaaaagtg
      961 caaatagttg attttaggtc atctaaatgt taaacataaa attattagta ctggtaaatt
     1021 tataaaaact attccaaaaa tctttttgaa tatttcaaaa cacaataaat tacttcaaat
     1081 ccccttgaat atattttaca gcattataga ttgctttaaa tctctttgaa tattctacaa
     1141 gactatagat tagtttaaat ccctttgaat atattttaca acattataga ttgttttaaa
     1201 tctctttgaa tattctacaa gactatagat tagtttaaat ccctttgaat atttcaaaac
     1261 accatagatt actttaaatc cctttgaatg ttctataaca ctataaatta cttttaactt
     1321 ttatataacc ataaagtttt tatctggcac tttaaatgga gttttatatc aaatttgcct
     1381 tccatcgaat aaccaaactt aatttaatta cacattccaa gattcaaata ttcattcaat
     1441 atatttgata attctatgtt atagctataa ttaaatatag attatt
//
LOCUS       DAMJYC010000239         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJYC010000239 DAMJYC010000000
VERSION     DAMJYC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.249"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            27..101
                     /locus_tag="PC472_004048"
     tRNA            27..101
                     /locus_tag="PC472_004048"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC472_004049"
     tRNA            116..200
                     /locus_tag="PC472_004049"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC472_004050"
     tRNA            210..293
                     /locus_tag="PC472_004050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC472_004051"
     tRNA            323..399
                     /locus_tag="PC472_004051"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC472_004052"
     tRNA            407..482
                     /locus_tag="PC472_004052"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC472_004053"
     tRNA            571..659
                     /locus_tag="PC472_004053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC472_004054"
     tRNA            663..738
                     /locus_tag="PC472_004054"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC472_004055"
     tRNA            745..818
                     /locus_tag="PC472_004055"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC472_004056"
     tRNA            833..909
                     /locus_tag="PC472_004056"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC472_004057"
     tRNA            933..1009
                     /locus_tag="PC472_004057"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC472_004058"
     tRNA            1017..1093
                     /locus_tag="PC472_004058"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC472_004059"
     tRNA            1102..1177
                     /locus_tag="PC472_004059"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC472_004060"
     tRNA            1185..1258
                     /locus_tag="PC472_004060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC472_004061"
     tRNA            1265..1336
                     /locus_tag="PC472_004061"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJYC010000240         1275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJYC010000240 DAMJYC010000000
VERSION     DAMJYC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.250"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            44..307
                     /locus_tag="PC472_004062"
     CDS             44..307
                     /locus_tag="PC472_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729291.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(744..1121)
                     /locus_tag="PC472_004063"
     CDS             complement(744..1121)
                     /locus_tag="PC472_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2729292.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          522 a          169 c          109 g          475 t
ORIGIN      
        1 ttattttttt gagatgatat aattttctta agaggtggtt gatatgataa aaaatacaaa
       61 acaaattaca gtagaagtta atgaaatttt attacaagaa atgtctaaga taactaatga
      121 cgaaaataat attttgacag atttgataaa tgaatcaatt gtaaattata ttcttgaaaa
      181 caaaaaacat aatattgaaa ataaagattt agaagaagtg attaaatttt acaaaaaaga
      241 aaatactgaa aaacctaaaa gtgcagcaag agctatagct gaagcaaaaa ctcatactct
      301 gtattaatca aataaaaaag ttcctaaaaa ctaggaactt ttttatatta ataattatta
      361 atatatctca atatttatct caaatctatc aatataagca tgtttaatac aatctattgt
      421 atacttatct taccctagaa ataatcaaaa caaaaataaa attatgccct ttatatctaa
      481 aacaattaat ttacatgatt aatatacaaa aattctgact tttaaattcg tatacaatat
      541 caaactaaaa taatattaat gacaaaccca atataaatta aattcaaaca ggtttgattt
      601 tacatcaaac ttattgtatt aatattaaat aacaagacaa aaatatttat aaaaatcaca
      661 aattttacat aaaactttat atttgattca tttaactaat tattgtaaaa actatttatt
      721 ttatgataat taactacacc aaattattca tcttttttaa tatctttaac ccatttttca
      781 acagagttca attcttcttc atttaccccc tttttgaaca aggatttcat aaaactctca
      841 atagaacttc catgcatatt atctaaaaaa gtttttgttt caaaatgtaa atactcttct
      901 tctccaataa gaactgtata atgtgtatgt ctatcaattt ttcttgcact taagaattgc
      961 ttatttacta atcttgataa aagtgttagt gttgtagttt gcttccaatt atatagtttt
     1021 tccatagctt caatgacatc ttttgatgct actgtaaaat ctgaactcca aataaatttc
     1081 atgactttta attctgctgg tggtatctta gtaatagtca tatgatttta cactcctctc
     1141 aaacttctac ataattaaat actatattaa tagacttttc gagtcaaagg ttttatttaa
     1201 aatatctcat ccaaatttat taatacaaaa aatttatatt tttattcaaa attaaacaca
     1261 caatctatta atata
//
LOCUS       DAMJYC010000241         1274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJYC010000241 DAMJYC010000000
VERSION     DAMJYC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.251"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..309
                     /locus_tag="PC472_004064"
     CDS             <1..309
                     /locus_tag="PC472_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2729293.1"
                     /translation="TIGFEVLKNYSSAFVSAPDWVSAKGMRILGNPLRGDEKVISGES
                     GAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKYRDIVWNGECQSK
                     "
     gene            659..>1274
                     /locus_tag="PC472_004065"
     CDS             659..>1274
                     /locus_tag="PC472_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2729294.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMA"
BASE COUNT          575 a          127 c          240 g          332 t
ORIGIN      
        1 actataggtt ttgaagtgtt aaaaaattat tcatcagcat tcgtttcagc accagactgg
       61 gtatctgcta aaggtatgag aatattagga aaccctctaa gaggagatga gaaagtaatc
      121 tctggagaat ctggagcagt tacaactgga gcattagttt caatccttga aagtgaagat
      181 ttaaaagact taagagaagc tttgaaacta gatgagaact ctaaagtatt attaataagt
      241 acagaaggag atactgaccc tgataaatat agagatatag tttggaatgg tgaatgccaa
      301 agtaaataaa agacttaagg aaaagaaaat cttaacaaat aaatgaaaaa tttaaggaaa
      361 ataaaaactt aaataagtga aaactttaaa taaaataaaa actaaaacaa atgaaaaatc
      421 taaataaatg aaaaagtaaa gcaaataaaa aagtaaagca aatgaaaaat tcaaaataaa
      481 aactaaataa aattaaataa aaccaaataa aattaaaata aagtctaaat aaaaatttaa
      541 taaataaaat ataaagaaat aaaaaatcaa tagattaagt gaaatgctaa taaattaaaa
      601 aagtgaaata tcaaaataaa acgtcaacaa attaaaaatt atatggggga atatcaaaat
      661 gttaaatgaa acaagaaaaa aacaattaac tgaagtatgc caagacttaa taagaaatgc
      721 tagttattca ggtcaagaag aaaacgtagt aaaagctata gaagaaaatt ttgctttact
      781 aggttttgat agttggtcaa gagatagata tggaaatata gtaggatgta taaaaggtaa
      841 caagccaggt aagaaaatat tatttgacgg gcatatagat acagttccag ttccagatgc
      901 aagtaaatgg agtgttcctc cttttgaagc gagaatagta gatgggaaaa tatatggtag
      961 aggaacttct gatatgaagg ggcaagtaag tgctatgatg gctgctgttt catactttgc
     1021 agaagatact aacaaagact ttgaaggtga attgtatgta gcaggtgttg ttcatgaaga
     1081 aatatttgaa ggtgtttcag ctagagagat aagtaaggct gttcaacctg attatgttgt
     1141 aataggtgaa tcttcagaat taaacctaaa gataggtcaa agaggtagag gagaaatagt
     1201 agtagaaact tttggtaaac cagctcactc tgctaatcca gaaaaaggta taaatgcagt
     1261 ttataaaatg gcta
//
LOCUS       DAMJYC010000242         1265 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJYC010000242 DAMJYC010000000
VERSION     DAMJYC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.252"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            171..1247
                     /locus_tag="PC472_004066"
     CDS             171..1247
                     /locus_tag="PC472_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2729295.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          499 a          175 c          222 g          369 t
ORIGIN      
        1 gacttatgaa aaggtagaaa atgaatgttt tcataaaata tataaaataa attatatata
       61 aaaaataaat tttatgttga catagttaat tcagcttgat attatagtta ttaaaaataa
      121 ggacagtaaa aacgctatcc atattacaaa ctaaaaaggg gggaattata atgagtaaga
      181 aattatttat tcctggtcct attgatgtta aagaagaagt cttgcaaaag atggctacac
      241 caatgatagg acatagagga aaagatgcat ctatgctcca aaagagtata agtgaaaaga
      301 tgcaaaaact attctataca aacaacacga tacttttatc cacgtcatcc ggaactggtt
      361 taatggaagg ctccattcgt tcctgtacat ccaaaaaggc agcagtattt tcctgtggat
      421 cctttggtga tagatggtac aaaatggcag tggctaataa tgtcccagcg gatatattta
      481 aagttgaatt aggagaggca acaacgccag aaatggttga taaggtgctt tctaccggag
      541 aatacgattt aataactgta actcacaatg aaacttcaac tggaataaga aatccaatag
      601 aagaaattgg agaagttgtt aaaaagtatg aggatgtaat ctactgtgta gatactgtaa
      661 gttcagccgg tggaataaaa gtcgaggttg ataagattgg aatagatatt tgtataacat
      721 cagtacaaaa agcactggga ttaccaccag gaatgtctat ttgcacattt tctcaaaaag
      781 caatcgatag agctaagcaa gtacctttta gaggagtata tcttgatttg ttagcaatgt
      841 atgaatactt aatcaagaaa aactaccaat acccttcaac accatctctt tctcacatgt
      901 tcgctttaga tttccaatta gataatatat tagatgaagg cctagataac agattcaaca
      961 gacatgaaga catggcaaac ttagttagaa actgggctaa aaaacacttc caaattttta
     1021 ccaatgaaaa tcacttatcc aatacactta ctgttataga aaatactcaa ggtattagtg
     1081 tatcaaattt aaattcaaaa ttacaagaac gaggattcca aatagcaaat ggctatggag
     1141 atttaaaaga aaaaactttt agaatttcac atatgggtga ctatactgtc gaagatgtac
     1201 aagaattatt agataatatt gatgatatat taggttttaa taaataatat tgtaaaaata
     1261 tgtat
//
LOCUS       DAMJYC010000243         1173 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJYC010000243 DAMJYC010000000
VERSION     DAMJYC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.253"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            61..1101
                     /locus_tag="PC472_004067"
     CDS             61..1101
                     /locus_tag="PC472_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2729296.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          461 a          140 c          192 g          380 t
ORIGIN      
        1 tcagaacatt tgtatttatg ttataatttg tataaatata tttatttagg aggaaacaat
       61 atgaatttaa atttaactaa tactataaat ttaatgaaaa aatatttgga cattccaagc
      121 ccagctggat atactgaaaa tgcagtttta gaaatcaaaa aagattttga aaatttaggt
      181 cttgacacta ttcttacaaa aaaaggtgca cttatagcta ctcttcctgg tgaagatgat
      241 tcaaatcaaa taacaatatc tgcacatatg gatacacttg gagctatagt aaaagaaata
      301 tcatctgatg gtactcttaa ataccataaa gttggtggtg gatgttgggc agctatagag
      361 ggagaaaact gtactgttat aactagaaaa ggtaaaaaaa tcagaggaag tgttgtattt
      421 aagtatgctt ctactcatat atatggtcaa acaaaagctg ccactgaaag aaatgaagaa
      481 acaatgatta ttagacttga tgaaaaagta tttacaaagc aagatgtatt agatttagga
      541 ataagtgttg gggattttgt atgcctagac catagatttg aggctacaga atctggtttt
      601 ataaaatcta gatatataga taataaatca gctgtagcaa tggttcttga aatatgtaga
      661 tattttaaag aaaacaattt aacacctaag catactacta acttttatat aagtaattat
      721 gaagaaattg gacatggtgt ttctaaatgt ttacctgaaa aaacaaaaga atttgtagca
      781 atagatatag cacctactgg accaagtcaa acatctagtg aacatggtgt aactatagtt
      841 gcaaaagata atttatcatt atatgatttt aatttaagaa acaagcttgt cgatgttgca
      901 gaagacaata atttagacta cgctgtcgat gtatttactt tctatggttc agatgcttca
      961 gaagctattc gttggggaga agatgttcaa atagcatgtg ttggtcctgg aacaaacaat
     1021 agtcaccatt atgaaagaac acatatagaa gctattgaaa atacactaaa attacttata
     1081 aattatatgc ttaaagaata attaattata tattttacca aagtcaattc ttaacaaata
     1141 attaacttta atttttattt ttatacaaaa att
//
LOCUS       DAMJYC010000244         1149 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJYC010000244 DAMJYC010000000
VERSION     DAMJYC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.254"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(301..1047)
                     /gene="cdtR"
                     /locus_tag="PC472_004068"
     CDS             complement(301..1047)
                     /gene="cdtR"
                     /locus_tag="PC472_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2729297.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          430 a          139 c          119 g          461 t
ORIGIN      
        1 tttcataaaa tatgtaggta tatatcataa aaatatgtat ttatatgata tgtcagtaca
       61 aaataacaac tgaatatttt aaagagacta tttcgtactt ttatgattta atgagctatc
      121 ttatcgaatc ataatctagg aacaattgtt ataatatcta atagtagtta tatatgatat
      181 agtcagtatg tgttttttca caatatgatt attatataga ggattcttag ggattactat
      241 ttttgtataa aaaagagatg gttcaaattt ttatcttgag acatctcctt tttctattta
      301 ttatgtttta ataatattct ttaaaatatt tctatatgta ttagatatac gacatatatg
      361 gccattactc attttaatat tatttttctt taggtcgaga gaaacaatat gatatttatt
      421 aactacaaaa gatttatgta cacggataaa gcatgcatct aaatctggta aagactttaa
      481 acttttatag aattcaaata ttctagaagt agtcactaat tttattttat gtggaatagt
      541 gcttgtttca aaatatataa cttcatcaaa tgaaattttc catagtgaga aatctgaata
      601 gatagtaaaa aaattagagc gataattttt ttctttaatg tatcttttgt gagcaatttt
      661 aatacaagct tttattttac tttcagcaat ttccatatca tgttttagta taaagtccat
      721 aacttctaac ctataatcga agatccctaa aatcatttct atatgatttg taagaaatat
      781 gattggacta atataatctg attctctaat tttttgtgct atccataatc catcacataa
      841 ttcattactt atcttatatt ttgcgtctac aactataaaa tatattctta ctttattttt
      901 ttcagtaagc tcttttaata acaaatttgc attatgatat aatcttacaa catcattatc
      961 aaaaccttct tttattaaaa tattatttat tttttctttt agtttcattc caaaacaaac
     1021 atcattatca aagattaata tatccactaa ataccctcct ataaaaaatt caaaagtaaa
     1081 aaaacaaaag tattttatag aaaatacata aaataacact aggagataaa taagattaag
     1141 gatagttag
//
LOCUS       DAMJYC010000245         1037 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJYC010000245 DAMJYC010000000
VERSION     DAMJYC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.255"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            50..910
                     /locus_tag="PC472_004069"
     CDS             50..910
                     /locus_tag="PC472_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2729298.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          380 a          110 c          154 g          393 t
ORIGIN      
        1 aaattggaaa aactatataa tatatattat aatctatata taaggagtga tgttattgaa
       61 aaaagataga ataacttttt cagaaatttt aatattaatt gtaggctgta tattaatggc
      121 catttctctt aacttatttt ttaatccaca tgcaatcgct gctggtggta taacaggact
      181 tggagttgtt ttaaattcat tattcggtgt agaactttgg attgtcaatt tacttttaaa
      241 tgttccatta ttcatatttg cttacaaaat actttcaaaa aaagattgtt ttaaaacggt
      301 tttaggaata atttttctta ccatagcact taagcttact gcaaatatgg ctacattaga
      361 tataacaaat gatatgtatc ttgcaattat atctggttct attttaatgg gagttggtca
      421 aggacttatt tttagaataa atggctctac aggtggaact gatttaatgg cactcctact
      481 aaataaatat tttcctacat ttagtatacc tgttttaatg ggtattgtag actgcgttgt
      541 agttgtttta tcaggtatag taaatagaca ggttgaaatt gcactttatt caactgtggc
      601 tttatacatt ttagtaaaag ttagtgactt gcttattgaa ggatttaatt attccaaatc
      661 attcactatt atatctgacc tttcaaaaga tataagtaaa aaaataatgg aagatttaga
      721 tagaggtgca actattctaa aaggagaagg tgcatatact ggagaaaata aaaatgtact
      781 tttagtagta gttgaaaaga aagaagttgt cgaattaaaa aaattagtaa aaaatgtcga
      841 ccctaatgct tttattataa ttacagatat acatgaagct ttaggtaatg gatttaaaaa
      901 aatagaataa ctaacaaata aatagcaagt ataattaaca tatacaaatt caacttttta
      961 tatgttatat tatacttgct atttatatat tatactttct atttatatat tataattgtt
     1021 gtttttaaga taaaaat
//
LOCUS       DAMJYC010000246         1005 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJYC010000246 DAMJYC010000000
VERSION     DAMJYC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.256"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            41..997
                     /locus_tag="PC472_004070"
     CDS             41..997
                     /locus_tag="PC472_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2729299.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          411 a           85 c          153 g          356 t
ORIGIN      
        1 aaaattttta gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa
       61 tacaagagag actattgaag attttagaga taaattttat gtagcagaaa attcttatct
      121 tatattaagt gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat
      181 aacttttaat gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta
      241 cgatttttta agcctaaata catttgaact aagagatgga gaagctgtta tagaagaggt
      301 aaatatgtat ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt
      361 tgaatttgta aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa
      421 tctatttaag ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt
      481 ggaaaaagtt gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa
      541 gaatcatgta tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac
      601 tagacctctt ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata
      661 ttcaaatgta aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa
      721 attatacaat ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc
      781 ttccagagta ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat
      841 atcagcacca cttacaatta taactggtat atatggaatg aactttagat atatgccaga
      901 attaaactgg atttatggat atccagcaac tttatttttt atgctatgta taatttttgt
      961 tggaattata atatttaaaa taaaaaaatt attgtaaatt acaga
//
LOCUS       DAMJYC010000247         1000 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJYC010000247 DAMJYC010000000
VERSION     DAMJYC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.257"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            101..664
                     /locus_tag="PC472_004071"
     CDS             101..664
                     /locus_tag="PC472_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729300.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
BASE COUNT          411 a           94 c          143 g          352 t
ORIGIN      
        1 aaatcaaaat ttgtaaaacc acatttatta tgttagaata tcttatgata ttaaattgta
       61 agtgtataat attatttagt tactagattg gggggaaatg atgaataaag tctatattgt
      121 aacaacatat acaggaacta tattatctta cttaataagg aatataagta aaaagctgta
      181 tacacatgtg tcaatatcat taaatgaaaa tctaaaacca atgtattctt ttggaaggct
      241 aaatccaagg aatccattta ttggaggatt tgttgaagaa aatataaatc aaggattata
      301 tgctataaga aaaaacactg tgtgtagggt atattcgtta gaagtagata atttacaata
      361 tgaaaatcta tataaaaata taaaattaat tagtgattat agagaagatt actattacga
      421 tacaatggca ttaatatatt tactaatgaa tattcagaga caatctgaat ataaatatgt
      481 atgttcaaat tttgttgctg acatgttaga aaaaagtgag atagacattt taaataaaca
      541 agcatttgaa gtaacgccaa atgattttta taatttatct ggcttaacct tagagtatga
      601 aggtttatta tcagaatata attctagaca acattcttat gtatcaaaaa tcgcaaacat
      661 ttaaaaactt gatttagagt ttttcatata aaaaaatatt gctagataat taggctgaag
      721 atagtagttt aacttcgata ggattaagaa tttgtaaatc ttagttagaa tactttcttc
      781 agtttttaaa tttctgctaa tatttgtatt tttataatat ttttggatat atataataca
      841 gaatagtgca aaaatcccac tcctttaggt gcgggatgga tagcacaaac agtgtataaa
      901 aagtatttat acatacacgt ataatgttaa atatagttga ataagcagaa ctataaatat
      961 ataaagatat cttaatatat aataaatatg ataaatattt
//
LOCUS       DAMJYC010000248          967 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJYC010000248 DAMJYC010000000
VERSION     DAMJYC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.258"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(798..>967)
                     /locus_tag="PC472_004072"
     CDS             complement(798..>967)
                     /locus_tag="PC472_004072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729301.1"
                     /translation="PPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTTVSSLLG
                     RIAVNIFNFNK"
BASE COUNT          380 a          112 c          101 g          374 t
ORIGIN      
        1 caaaaatttt taattagact tctctcttat tcaagatttt tctatataaa atctaagaat
       61 aaatagaaaa ctaacatcta accttttatt ttttattttc gatctattta cttgaatttt
      121 aacataaatc tatcttttag tatatattaa tcatgctgaa tttgtgatga catgtataca
      181 aaatatcgtt tagtattttc taataaaaaa tattactgaa attaaaaatc tacaatagca
      241 tttaacaagt aaaaacatgt ttacacaaat atataattta aggtctttaa aatagagtat
      301 gtattattct aaaaattagt tatagttatc acataaaaat agaaaagaat tttcaaaaga
      361 tttaaagaac atatatactt caattgcaga agatgtaatg ttaagtgaac tagcattctt
      421 cgaggaaaaa tagactcaaa aaattagaaa atagaatcag gttttagatg atttaagtat
      481 ttattttcaa aaaagaaatt tgattctaag ttaataaaga aatatttaaa aaaatcaaaa
      541 aattttctta aataagtaat aggttttgta aaaatggaaa aatatcaaaa taagactatg
      601 atttttaaaa ctatagcctt atcaataaat ttttatttaa agaacacaca taaatatttt
      661 taaacctaat ttatgtgaag ttttatgtat tttttatata agtagtattc taatcttact
      721 catctaatat accaaaaaga ctgtaagtaa gttcactttt atgcaaactt acccacaatc
      781 tcttttacac attaattcta tttattaaaa ttaaaaatat ttactgctat tctacccaat
      841 aaagatgata ctgttgttgt tctggctggt actgttactt ctatttctcc tgtatctgct
      901 gttttaccac ttgatgtgtg tgtcactgta aatactaatg ttacttttgt ttctgtatct
      961 ggtggca
//
LOCUS       DAMJYC010000249          837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJYC010000249 DAMJYC010000000
VERSION     DAMJYC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.259"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     regulatory      <1..64
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            147..632
                     /locus_tag="PC472_004073"
     CDS             147..632
                     /locus_tag="PC472_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2729302.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          370 a           72 c          128 g          267 t
ORIGIN      
        1 atgacgcaaa actataggga ctaaaatctt tatgtaataa aggtcatgtc agccagttgc
       61 caaaaagata ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt
      121 aatatttggc aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttcgca
      181 aatctagcat caattttaag actaaaatta aattgaattt aagagaaaaa ccagacataa
      241 atagtttgaa attaaaaagt ataccagaag gtaaaattgt taagttaaag tgtgtagatg
      301 gaatatgggc agaagttgag agtaattatg acaaaggttg gttactttat aaatatttag
      361 aaagattaag taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt
      421 acataggaaa tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag
      481 aatctaaagt tataactaca atccctgatg gttttaaagt tgaagtatgt tatagcgttg
      541 gaaaatgggc aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata
      601 atcaatacat agaagagcat ttaagtttat aatacgatac tagcaagctt taaatatatt
      661 aataaatcgc aaaaaagaat cccattaatg ggattctttt ttgtagaaac attataacag
      721 aactaaagag aataaattaa ctaatttatt tttattataa agtaattata aatggtaaat
      781 ttaattgaaa gtagatttaa cttatacttt atacaccaag tatcataaat taatact
//
LOCUS       DAMJYC010000250          837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJYC010000250 DAMJYC010000000
VERSION     DAMJYC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.260"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            184..690
                     /locus_tag="PC472_004074"
     CDS             184..690
                     /locus_tag="PC472_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2729303.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHDNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          341 a           80 c          137 g          279 t
ORIGIN      
        1 attttatcta tattttacac ttataaaata tgaaaactac ttattgcgaa agtaattgat
       61 ttgttgtatt ataatttaca tgaattatgt aggacaatca aaagtcagta ttataagagt
      121 acatatattc aaatttttaa caattaattt atataattaa aaaatgaagg gtaggatgaa
      181 gtcatgggtt ttagagaagt aaaaattgag gaattacagt ttaatccatt tactaagatt
      241 ggcaaagaat ggttacttat aacagctggt gattcagaaa agtttaacac aatgactgct
      301 agctggggtg gtgttggagt atattggggt aaaaatgttg ttacaactta cataagacca
      361 cagagatata caaaagaatt tgtagatagt aatgatacat ttacagttgc cttctttgat
      421 gaaacgtata gagaggcttt aaatatttgt ggtactattt ctggtagaga tataaataaa
      481 atagaaaaag caggacttac accatatttt gtggatgata cggtagcttt tgaagaagct
      541 aatatgatta tagtatgtaa aaagctatat catgataata tgccaccaga aaactttgat
      601 gctaaagaaa atgataaaaa atggtatcca gagaaagact accatactat gtacatttca
      661 gaaattataa aagttcttgt aaaagaataa ttataataaa aaatatatga aaagaactta
      721 ttataagtaa atgactgtgc tttcttatct gatatctaag tagatgctag taactaaata
      781 aatattaatt ttaaataaat actattataa aaaagatgaa ctatatttac tatagtt
//
LOCUS       DAMJYC010000251          828 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJYC010000251 DAMJYC010000000
VERSION     DAMJYC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.261"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            51..518
                     /locus_tag="PC472_004075"
     CDS             51..518
                     /locus_tag="PC472_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2729304.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSMDEVAKRMDVTVEYIESVLE"
BASE COUNT          313 a           44 c          149 g          322 t
ORIGIN      
        1 aattctaatt atatattata aataaagtat gtgtatatta aggaggtaat atgaagtttt
       61 taaatgtttt aatagtagtt gaagatattg agaaatcaaa aaagttttat tatgatgttt
      121 tggggttaaa ggtaatctgt gattttggag aaaatgttgt attagaaggt aatatatcat
      181 tacaagaaaa aaagctttgg ttagaattta ttaataagag tgacagtgaa gttaagttca
      241 atggaaatga tgctgagtta tattttgagg aagataattt tgatactttt gttgaaagat
      301 tgagtacaat gaaagatata gattatgttc atctagctat agagcatagg tggggtcaaa
      361 gagcaattag attttatgat ttagatgggc atattattga ggttggagaa actatgtcat
      421 ctgtgtgtag acgtttttta gatagtggtc tttcaatgga tgaagtagct aaaagaatgg
      481 atgtaactgt cgaatatatt gaatcagttt tagaatagat ggattatatt aaagtaaaat
      541 tgaggtgaag gctgtaagta aacttgtgtg aaaacaaatt tgcttacagt ttttattttt
      601 ctaaaataaa gaatataata cttgtttagc tttatttgag tttttaaaaa tattatgata
      661 tttcttatat atttatacga aagtttagat ttagaaaaaa tatataaaag atagttatat
      721 ttactattat cttgtataat tatatatact attttatata actatgctaa aatttaattg
      781 aataaattaa aaatataatg aaagtttagt tttaattcat ttatttaa
//
LOCUS       DAMJYC010000252          825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJYC010000252 DAMJYC010000000
VERSION     DAMJYC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.262"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            94..636
                     /locus_tag="PC472_004076"
     CDS             94..636
                     /locus_tag="PC472_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2729305.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          345 a           67 c          141 g          272 t
ORIGIN      
        1 accttcttca attaatatat tattattagt aataacttta aaattatatt actattatat
       61 aaaaattggc ttaaaagaag gtgaaaaaag taaatgaaaa tatgcataac agtaggtcat
      121 agtatactaa aaggtggtaa aagtacaggt gtaaatggta tagttgatga atacaattat
      181 aataaaaaat tagctcctat gttagctgag atgcttataa gtcaaggtaa tacagtagat
      241 gtaataattt gtccagaaag gcactttatg tcagaaagag aagaattttt ctatagagtt
      301 ccaaaagtaa acagtggtaa atacgattta ttagtagagc tacatttaaa taaagctgat
      361 ggaacacaat gtggtacaga agtgctttac tatggcactg aaggattaga atatgctaaa
      421 agagtatcta atagacttgg agaattattt gaaaatagag gagctaaaaa aagagaaaat
      481 ttatatatat tgagaaatac aaatccagtt gcaatacaga ttgaaagttt tttctgtgat
      541 aatgtgaatg attgtaacaa agcaaatgaa tcaggttatg attatatagc acgattgatt
      601 acagaaggta tattgaataa agatataaat atgtgagatt atggtgattt aggtattgat
      661 taataccttt taatattaga tataaataaa aatacaatgt attagtataa tttaaacttt
      721 caaaagatag attagtttga ttattgacaa gagatacctc cttgaaaata ttgatttatc
      781 agtatattta aggaggtatt taaatataaa aatttaatta atttg
//
LOCUS       DAMJYC010000253          803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJYC010000253 DAMJYC010000000
VERSION     DAMJYC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.263"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..613
                     /locus_tag="PC472_004077"
     CDS             <1..613
                     /locus_tag="PC472_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729306.1"
                     /translation="QQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIE
                     DNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVY
                     DINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGI
                     VDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          339 a           61 c          123 g          280 t
ORIGIN      
        1 acaacaaaat atcaatgaaa ttaaattttc tattggaagt aaattatact tagaaatagt
       61 aaataataaa agtaaaaatg gatatattga aggacatgat agttactcta actttattat
      121 atatgatata gaagataaca cttttaatat aagtaataaa tttatggata atctaaacaa
      181 agaaaagaat gcaaatccaa aggagattgg cttattaagt aaaactaagc tagattatat
      241 ggtaaaaaat aataaattga atcttatagt tacggatatg agtaattcca taactaatct
      301 tgtttatgat attaattcta atgagattaa attagaagat tatgagatac ttgatttaaa
      361 agtagattat aatgaagcat catctggagt taggaaagtt aagctactgg atgataagat
      421 atattctatt tcaggtatta agaacagtat atcaggagaa ttaaaaggat ttaaagggat
      481 tgttgatgta gtcggaacta agccagtaaa atttcaagta tttgatatga aacttaaaaa
      541 gaatatttat gaagctgagc taataaatgg agatataagt ttacaagaaa agttattttt
      601 tgttgcaaat taagtgttag aattaatttt agaactttaa acttttatat gtatcacaga
      661 taagtataag taagggagtg tctcaaaata aactttttag ctcatgagac acttttttta
      721 tatgaaatca aatatatttt cattgtttag atttattttg ataatgaaaa aaagattatc
      781 tttattttaa gaaagacatt tat
//
LOCUS       DAMJYC010000254          801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJYC010000254 DAMJYC010000000
VERSION     DAMJYC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.264"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            58..>801
                     /locus_tag="PC472_004078"
     CDS             58..>801
                     /locus_tag="PC472_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2729307.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSE"
BASE COUNT          332 a           85 c          129 g          255 t
ORIGIN      
        1 attgtatttt atcttataat atttactata atactatatt gcgaaaagag gtagaaaatg
       61 tttgcagaag aaagaatcca agctatatta aatatcctaa aaaaagaagg tagagttact
      121 gtcaaagagc tcagttctaa atttaatgtt acagaagatt gtataagaaa agatttaaaa
      181 aatatagaaa agatttcttc aataagaaga atttatggag gagctgtact agctagagaa
      241 acactagaga atcaggatac aaaagacaga aaagaaatta atattccaac aaagaaaata
      301 atagcagaaa aagcatttaa tttaattgaa gatagagaaa ctatattttt agatatctct
      361 acaattaaca tcttgcttgc gaagttgtta gctgaaagta ataaaaaagt aacacttgtt
      421 acaaatatgc tggatatact aaatgtagtt acttcaaaac caaataattt aaatataatt
      481 tccactggag gtttattaaa tctcagttta gacggttttg tcggtactcc aactatagag
      541 tttatatcga aatataagtt tgataagact tttatgggaa gttgtggagt agatgtattc
      601 aactcttcac ttacaacttt tgaaattgaa gatggtctta cgaagaaggc tataataaat
      661 tcaagtaaaa aagtatttat agttatggaa gataaaaagt ttaaatttga tggaaatttc
      721 aagtttgctc atttagaaga tattagttct ataattacag aaaaaacgcc tactcctgat
      781 atagtagata ttttatcaga a
//
LOCUS       DAMJYC010000255          763 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJYC010000255 DAMJYC010000000
VERSION     DAMJYC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.265"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            73..>763
                     /locus_tag="PC472_004079"
     CDS             73..>763
                     /locus_tag="PC472_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2729308.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTL"
BASE COUNT          283 a          106 c          127 g          247 t
ORIGIN      
        1 tatattacaa cattatttta tagtttgatt aatttaacaa tcactaatat atcaaactga
       61 aaggagattt ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg
      121 ggaaatcctt caagtaaatt atatatttac atacatggac aatgtggcaa taaagaggag
      181 gctgaagctt ttgctaatat tgcagttcca catgagtgtc aagttttaag tgttgatttg
      241 cctgaacatg gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca
      301 gaattagtat ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca
      361 aatagcattg gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt
      421 atttttgtat ctccaattct agatatgcaa cagctcattt caaatatgat gctttgggca
      481 aatgtatcag aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact
      541 ctttcatggg aatatctaca atatgtaaaa caacatccta tcactcaatg gaatgtacca
      601 acaaaaatat tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt
      661 gtagaaaagt ttaattgcaa ccttactatt atagaaaatg gcaaacattg gtttaacaca
      721 ccacaagaat taaattcatt atatgaatgg ttaaatactt tag
//
LOCUS       DAMJYC010000256          748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJYC010000256 DAMJYC010000000
VERSION     DAMJYC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.266"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            108..578
                     /locus_tag="PC472_004080"
     CDS             108..578
                     /locus_tag="PC472_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2729309.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTVIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          345 a           55 c          130 g          218 t
ORIGIN      
        1 taaaaaagta aaaataaagg attaataaaa ggtactgaag agatagagaa aaaaatgtta
       61 atagaagata ctgaaaaaat agaaataaaa aattaggagg aaaatttatg aaatatcagg
      121 tttcattatt agcagtgaag gatattgaag tatcaaaaaa attttacaaa gaattatttg
      181 agcaagaaat tgaattggac ttaggtaaaa atgtaacatt taaaggagga tttgctatac
      241 aagaagactt cgcatggctt acagggatta atccagatac agttatacaa aaatcaaata
      301 atatggaact ttattttgag gttgatgatt ttgacgaatt tattgaaaaa ctcaattatt
      361 ataaagatgt agagtttgtc cataagccac taaaacatga atggcaacaa agagtagtgc
      421 gtatttatga cccagataag catattattg agataggaga gtctatggaa gttatagcta
      481 gaagatattt atctgaggga tattctgtag aagaaacttc taaaataatt caacatccaa
      541 ttgaatttgt aaagcagtgt gaaaatagta aggaatgatt gtttaaatta taattattta
      601 gtagaaatta attgtggtag aactaattca agataatata taggactagt atttgacaaa
      661 tgtcataaat taaattaaaa taaagaaaat attaataaag aaaaaataaa taaaaataag
      721 gggaatatta ataaagaaaa aataaata
//
LOCUS       DAMJYC010000257          729 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJYC010000257 DAMJYC010000000
VERSION     DAMJYC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.267"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(11..679)
                     /locus_tag="PC472_004081"
     CDS             complement(11..679)
                     /locus_tag="PC472_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729310.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          237 a          121 c           38 g          333 t
ORIGIN      
        1 attagaataa ttaaactttt aatttattgt tatttaatct attttgataa acattattta
       61 ttttattatt atttaaatct ttaaaatcta aacttttaca ttgttcatca tcttcccata
      121 ttcctgcttg tttatttcca tcatttttat ataatacacc cttaccattt ttcttattat
      181 ctaaaaaata tcccttataa atgtcattat tgggatatgt taattttccc aaaccatttt
      241 ttaatccgta tttaaattca cctgtatata ttgaaccatc tctataagta tattttccta
      301 ttccatgcat cttgtcatct ttccataatc cttcatactt ttccttactt gaaaaaatat
      361 atattccaaa tccatttttc tttccatcaa caaactcacc tttatataaa tcaccattat
      421 cgtatttaaa tactttttct tcctttaatt tcttattgtt actcttattt ttattattat
      481 tgttatcctt tatatgtatt tcttctcttc tggcttcaat aaaatctcta tcatacctaa
      541 atccgtttct tgatttaatt ttttgattaa tcttcttctt attaataaat actctagcta
      601 gttgagtaaa tataaataca gatgataagc atataaatac acatagtatt gttgtaaaat
      661 aactagttat attttccata aaaaatcacc ctttaaaaaa tttatttaaa atacctaata
      721 taatatata
//
LOCUS       DAMJYC010000258          679 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJYC010000258 DAMJYC010000000
VERSION     DAMJYC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.268"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..140)
                     /locus_tag="PC472_004082"
     CDS             complement(<1..140)
                     /locus_tag="PC472_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729311.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIV"
     gene            complement(414..530)
                     /gene="rrf"
                     /locus_tag="PC472_004083"
     rRNA            complement(414..530)
                     /gene="rrf"
                     /locus_tag="PC472_004083"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(539..612)
                     /locus_tag="PC472_004084"
     tRNA            complement(539..612)
                     /locus_tag="PC472_004084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Gly,seq:tcc)
BASE COUNT          247 a          113 c           85 g          234 t
ORIGIN      
        1 acgatacttt ttttctctaa gtttatattt aaatcagcgt tattttccat agcattagat
       61 tcagtagcaa atgccattgt aggtgcgacg attgccattg aaagaatccc tgttgttaat
      121 aattttgtat ttcttctcat attaatctcc ttatttaaca taaatttcta ctttgttttt
      181 taacctttta atttttgaaa ttatatcaaa gtgtttttac ccaaaattat aaataagtac
      241 ataaaaaata aacttctaaa aaaattataa aaacaaaaaa taaaaaaatt aaatatattg
      301 aatatattta attatcttca ctatatcatt tattgccaaa aaaatctata aaagacaaat
      361 aaaaacatat aataattgta ggtttattaa atttaataca acaaaaaaag attacgtggc
      421 tacgtcctac tctcccaggc agtttcccac caagtaccat cagcgctaga gagcttaact
      481 tctgtgttcg gaatgggaac aggtgtatcc tctctgctat tgtaaccaca taatcttttg
      541 gagcgggtga aggggatcga accctcacag ctggcttgga aggccagaac tctaccattg
      601 agctacaccc gcattaaatt tttataaatt agtaaaacta attttttaga gttagcactc
      661 taaaaactac atatatatc
//
LOCUS       DAMJYC010000259          664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJYC010000259 DAMJYC010000000
VERSION     DAMJYC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.269"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..>664)
                     /locus_tag="PC472_004085"
     rRNA            complement(<1..>664)
                     /locus_tag="PC472_004085"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAMJYC010000260          659 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJYC010000260 DAMJYC010000000
VERSION     DAMJYC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.270"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>659
                     /locus_tag="PC472_004086"
     rRNA            <1..>659
                     /locus_tag="PC472_004086"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          187 a          136 c          195 g          141 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaagg
//
LOCUS       DAMJYC010000261          634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJYC010000261 DAMJYC010000000
VERSION     DAMJYC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.271"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>634
                     /locus_tag="PC472_004087"
     rRNA            <1..>634
                     /locus_tag="PC472_004087"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          178 a          138 c          183 g          135 t
ORIGIN      
        1 tgatggggag cgaaatttag tagcgaagta gctgatttca cactgtcaag aaaagtctct
       61 atcgaggtta aaggtacccg taccgcaaac cgacacaggt gggtgaggag agtatcctaa
      121 ggccagcgag agaactgttg ttaaggaact cggcaaaatg accccgtaac ttagggataa
      181 ggggtgccac catcaggtgg ccgcagagaa taggcccaag cgactgttta ccaaaaacat
      241 aggtttctgc taagtcgcaa gacgatgtat aggagctgac gcctgcccgg tgctggaagg
      301 ttaaggggat ctgttagagc aatcgaagca gtgaacttaa gccccagtaa acggcggccg
      361 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
      421 aggcgtaacg atttgggcac tgtctcaaca acagactcgg tgaaattgta attccggtga
      481 agatgccgga tacctgcgac aggacggaaa gaccccatgg agctttactg tagcttgaca
      541 ttgggtcttg gtactacatg tacaggatag gtgggaggct ttgaaaccag gacgccagtt
      601 ttggcggagc catccttggg ataccaccct tgta
//
LOCUS       DAMJYC010000262          587 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJYC010000262 DAMJYC010000000
VERSION     DAMJYC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.272"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
BASE COUNT          237 a           83 c           63 g          204 t
ORIGIN      
        1 tagctaccat cagcagacat atctggaaaa tctaagattt tgtaagttat ataaaccccc
       61 agtgccataa tcgataaaat taaactttgc gtcattacag atataatacc tgacataaat
      121 aaaacacccc tttgaaaata ttttaaatgg ttttttaaat ttggttttaa aaaatgattt
      181 tgatataaaa aaaccacaca gtatatataa ctgtatggca aaaattcgaa taagccacac
      241 agtataatcc atgtggctct atattatgat gataaaataa aaactttaac aacaattact
      301 atagccaaca tagatacaat ccttaaaaat cataatccta aggggcttgt atctatgtta
      361 tgacaatttg tcttaactta ggtacaaacc ctaactaaat tggctactaa aaaatcgtac
      421 ttgtttattt aataaagtct tcatatttac ctctcctttt aatttaataa ttttgaaaat
      481 tttgttttct gttttgtata gtaattagtt taatacagta aaaaataaaa gtcaactata
      541 aattttgaaa aattatcaaa ttatcagaaa tttgagttta aataaaa
//
LOCUS       DAMJYC010000263          578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJYC010000263 DAMJYC010000000
VERSION     DAMJYC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.273"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>578
                     /locus_tag="PC472_004088"
     CDS             <1..>578
                     /locus_tag="PC472_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2729312.1"
                     /translation="AFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLII
                     SSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVA
                     TVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIA
                     NLLSFFVLYDYIINLVKISPERRTAFLVIVLL"
BASE COUNT          200 a           52 c           79 g          247 t
ORIGIN      
        1 gcatttataa caattgtttc ttctattata gaaatatggt catgtaaaaa agtttttgat
       61 tatactagtc agtcaaagac cgacttaaaa aaactaaata tgtattttct cttttctgtt
      121 tttctcatta tttcttcttt atatataaat ataggagcta atagtagaat cttgatatgt
      181 atatttgtaa tgttcgtttt ctataagtct aactataagg ttaatatata taaatgtctg
      241 gtaatctcat ttttttattg gttaatatta ataggtataa atggattaag tatgttatta
      301 gtagcaacag taagcaattt ggataatatg aatgttttac ttagtggcag tttatataga
      361 tatgaagcaa tattcttggg aaagataatg ttaggtatat tactttatgt ttataaggct
      421 tcttttaaaa attttaaaat aagtaaaaaa gagatataca tatgtattcc tatgatagca
      481 aatttattgt cattttttgt actttatgat tatattatta atcttgtaaa aatctctcct
      541 gaaagaagaa ctgctttttt agtaatagtt ttactatt
//
LOCUS       DAMJYC010000264          571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJYC010000264 DAMJYC010000000
VERSION     DAMJYC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.274"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            259..>571
                     /locus_tag="PC472_004089"
     CDS             259..>571
                     /locus_tag="PC472_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2729313.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PL"
BASE COUNT          243 a           38 c           87 g          203 t
ORIGIN      
        1 ttatctttat acagtaaata aaaaatcaaa tgaaatacca aataaagtgt atatcttaaa
       61 ataacttact agagatacaa ggatgctatg tgaattttga agtgtgcagt ttagataata
      121 aatatatata aataataaag aaaatggatt tatagtattt aaaatttctt ataagggtaa
      181 aaaatgctat tagaaaatgt actaaaaata tttttccaat tctttataga aaaataatga
      241 tatttaggag agattgtaat gcaaattgat gtattgataa ttgatggaga taagaatagt
      301 tctcaagagc tgaaaaattt tttagaagga aaggggataa atgtctattt agcatataat
      361 tgtaaagatg ctattataaa aatatttagc aagaaatttg acttaatatt tttagagata
      421 attttagttg atggtgatgg ttgggatttt tgtaaaaaaa ttagaaatat aactacttgc
      481 ccaattattc atataactta tataaatgaa aatcaaagta ttttaaatgc tttaaattct
      541 ggaggagatg attatttaat aaagccttta a
//
LOCUS       DAMJYC010000265          555 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJYC010000265 DAMJYC010000000
VERSION     DAMJYC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.275"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..309
                     /locus_tag="PC472_004090"
     CDS             <1..309
                     /locus_tag="PC472_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2729314.1"
                     /translation="TIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNPLNGDEKVISGES
                     GAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKYRSIVWDGEYPSI
                     "
     gene            436..>555
                     /locus_tag="PC472_004091"
     CDS             436..>555
                     /locus_tag="PC472_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF2729315.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEK"
BASE COUNT          236 a           55 c          105 g          159 t
ORIGIN      
        1 actataggat ttgaagtatt aaaaaatcat gcagcagcat ttgtttcagc accagactgg
       61 gtttctgcta aaggtatgag aacattaggt aatcctttaa atggtgatga aaaggtaatt
      121 tctggagaat ctggagcagt tacaactggt cttttagtag cagctatgga aagagaagat
      181 ttagcagatt taagaaaaga tttaaaatta gatgaaaatt caagaatact tttaataagc
      241 acagaaggag atactgaccc tgacaaatat agaagtatag tttgggatgg agaatatcca
      301 agtatataag gaattactaa aatgcaatta ttagatattg gtttacatat ttagaagata
      361 tataagttct aaaatttaga aaatatataa gtatataaaa tacatacatt ataaaaaatc
      421 tagggggata atgaaatgtt aaacgaacaa agaaaacaag aagtaactga aatatgccaa
      481 aaattaattc aaaatcctag ttcttctgga aatgaagaag gtgttgtaaa agcaatagaa
      541 gagtcttttg aaaaa
//
LOCUS       DAMJYC010000266          531 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJYC010000266 DAMJYC010000000
VERSION     DAMJYC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.276"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>531
                     /locus_tag="PC472_004092"
     rRNA            <1..>531
                     /locus_tag="PC472_004092"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          144 a          112 c          175 g          100 t
ORIGIN      
        1 accaaggcga cgatcagtag ccgacctgag agggtgatcg gccacattgg aactgagaca
       61 cggtccaaac tcctacggga ggcagcagtg gggaatattg cacaatgggc gaaagcctga
      121 tgcagcaacg ccgcgtgagt gatgaaggcc ttcgggtcgt aaaactctgt cctcaaggaa
      181 gataatgacg gtacttgagg aggaagcccc ggctaactac gtgccagcag ccgcggtaat
      241 acgtaggggg ctagcgttat ccggatttac tgggcgtaaa gggtgcgtag gcggtctttc
      301 aagtcaggag tgaaaggcta cggctcaacc gtagtaagct cttgaaactg ggagacttga
      361 gtgcaggaga ggagagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga
      421 acaccagttg cgaaggcggc tctctggact gtaactgacg ctgaggcacg aaagcgtggg
      481 gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgagta c
//
LOCUS       DAMJYC010000267          481 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJYC010000267 DAMJYC010000000
VERSION     DAMJYC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.277"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>481
                     /locus_tag="PC472_004093"
     rRNA            <1..>481
                     /locus_tag="PC472_004093"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          115 a          111 c          151 g          104 t
ORIGIN      
        1 actgtcaggt gggcagtttg actggggcgg tcgcctccca aaaggtaacg gaggcgctca
       61 aaggttctct cagtacggtc ggaaatcgta cgtagagtgt aaaggcaaaa gagagcttga
      121 ttgcaagaca tacaggtcga gcaaggatga aaatcggact tagtgatccg gtggttctgc
      181 gtggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      241 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcatca catcctgggg
      301 ctgtagtagg tcccaagggt tgggctgttc gcccattaaa gtggtacgcg agctgggttc
      361 agaacgtcgt gagacagttc ggtccctatc cgtcgcaggc gtaggaaatt tgagaagacc
      421 tgtccttagt acgagaggac cgggatggac gtacctctgg tgtaccagtt gtcctgccaa
      481 g
//
LOCUS       DAMJYC010000268          468 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJYC010000268 DAMJYC010000000
VERSION     DAMJYC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.278"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..394)
                     /locus_tag="PC472_004094"
     CDS             complement(<1..394)
                     /locus_tag="PC472_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2729316.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNT"
BASE COUNT          164 a           63 c           55 g          186 t
ORIGIN      
        1 ttgtattaaa gtatacagtt ccattacttg aattattttg gttagctaat gctaataatt
       61 cagaattaga ataataatct ttatgctctt ttggttctaa aaacccataa actctatttc
      121 cactagaatc taaaatttgc aaccacaatt gattttctcg aagtttttct aatccagact
      181 gttttacttg tggcttatta tctataaata cgatttgttc attaaagttt tctgtaaatt
      241 ttttcggcca gtcactacga ataaattccc catttggttt ctgaatagta ataagtgata
      301 agaaaatacc tattactgtc aggattaagc aaataaaaat catgaaaaaa ataatgtatg
      361 aatgaaatac tgttttataa ctaggtttat gcatttaatc aactcttttc ttaaactttt
      421 attttgatta ttgcttatat aaaatagaca ataattattg atatgtaa
//
LOCUS       DAMJYC010000269          444 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJYC010000269 DAMJYC010000000
VERSION     DAMJYC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.279"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            28..>444
                     /locus_tag="PC472_004095"
     CDS             28..>444
                     /locus_tag="PC472_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729317.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLCNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQD"
BASE COUNT          183 a           44 c           61 g          156 t
ORIGIN      
        1 attaaatatt tatgaggaga ataaattatg aaaatacatt caatacaaaa tttaacacta
       61 gatacgcgta accttataaa aaaatattct cttactttaa atgaggattt aatttgggag
      121 tttaaacatg gtaaatacca cacgataaaa tactttacac ataagtttgc agttaaggag
      181 tctgttcttg cacttttgtg caacatacac aaattatgtt atgctaaaat aaaatatttt
      241 gagtcgagtt tttcaaaata cgaaccttat aaatatagtt atcaaaatga atttgttaaa
      301 tgtgaaatgt atgatatgaa ttttatgttt cataaatata gtaatataat gatagatttg
      361 agaaatttac aagaaattaa aaccatagaa gaatttagat tattttgtga ttacctagaa
      421 agctttgaag ataatttaca ggat
//
LOCUS       DAMJYC010000270          429 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJYC010000270 DAMJYC010000000
VERSION     DAMJYC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.280"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..306
                     /locus_tag="PC472_004096"
     CDS             <1..306
                     /locus_tag="PC472_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729318.1"
                     /translation="NMGIGTLVLGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTI
                     YFAMSLGMLSTDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            293..>429
                     /locus_tag="PC472_004097"
     CDS             293..>429
                     /locus_tag="PC472_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729319.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          163 a           59 c           62 g          145 t
ORIGIN      
        1 aatatgggta taggaacttt agttcttgga attgcctcta tcataattgg tattacacta
       61 tttaagaaat ttacttttat caaagatact actgcaataa ttgttggctc ttttatatac
      121 caattcacta tatactttgc aatgagttta ggaatgttat caactgactt aaaactaata
      181 actgccatag ttataatagc tttcctagct actggtaact taaatatttc actaaaaaaa
      241 aacaaatgca aagctagtac caaaaataaa tcagaaaaaa gaggtgttgt agatgttaca
      301 aattaaaaat ctttcaaaga gtttccctaa tccatatggt gaacctaata ctatctttga
      361 aaatttatca atcgatatag aagatggtga atttgtaagt atcataggta gtaatggaac
      421 tggtaaatc
//
LOCUS       DAMJYC010000271          417 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJYC010000271 DAMJYC010000000
VERSION     DAMJYC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.281"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            5..346
                     /locus_tag="PC472_004098"
     CDS             5..346
                     /locus_tag="PC472_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HDF2729320.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
BASE COUNT          175 a           41 c           74 g          127 t
ORIGIN      
        1 ttaaatggct aatatatatt gtgaaaatta taattgtaaa aactactttg aagatatgtg
       61 tatgcttgaa agaattgaaa ttaataactt caaagaatgc gaaagctatc ttgaaggtaa
      121 aaatgagcta tatgaattag aaaacggata tactatacat cctaaagatt tgaaaatggt
      181 gaaaagtaaa gattattctg ttgaagttac tcatattcca actggtatta cagtaaaatg
      241 ccgttctaca aatagtattt taaaaaataa aaataagtgt ttggaagttc tagaagaaga
      301 actaacaaaa ataaactctc acttagagct agaagattta cgctaaatag gaagtgagct
      361 tatgaaacga agaagatgca gttggtgtgg taagttattt tatcttgaag aaaaatc
//
LOCUS       DAMJYC010000272          391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJYC010000272 DAMJYC010000000
VERSION     DAMJYC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.282"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="PC472_004099"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="PC472_004099"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="PC472_004100"
     tRNA            276..364
                     /locus_tag="PC472_004100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
BASE COUNT          131 a           69 c           83 g          108 t
ORIGIN      
        1 taactctata tttttaaact tgtcaaaact tatggatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacacaata agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 actgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag t
//
LOCUS       DAMJYC010000273          386 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJYC010000273 DAMJYC010000000
VERSION     DAMJYC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.283"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..255
                     /locus_tag="PC472_004101"
     CDS             <1..255
                     /locus_tag="PC472_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDF2729321.1"
                     /translation="IKLENKMIKLTPTENRLLNYFIENAEKTLTIKEIYEHVWMNEYL
                     DDNYSVVVAVSGLRKKIEKDYKNPQKIITIREVGYYFNKI"
BASE COUNT          173 a           24 c           65 g          124 t
ORIGIN      
        1 ataaagttag aaaataaaat gataaagtta actccaacag aaaatagatt attaaattac
       61 tttatagaga atgctgaaaa gactctaact attaaggaga tatatgaaca tgtatggatg
      121 aatgagtatt tagatgataa ttatagtgta gtggttgctg tgagtggatt aagaaagaaa
      181 atagagaaag attataaaaa tcctcaaaaa attataacga taagagaagt agggtattac
      241 tttaacaaga tataaagctt tataaacaga atgaatgata aggcatagat aatatgttag
      301 tatattataa gagtttttgt tctaacgata tatttgtaaa attagattta atttacttaa
      361 ttaaattata gcagtagaat aaatat
//
LOCUS       DAMJYC010000274          378 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJYC010000274 DAMJYC010000000
VERSION     DAMJYC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.284"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            182..298
                     /gene="rrf"
                     /locus_tag="PC472_004102"
     rRNA            182..298
                     /gene="rrf"
                     /locus_tag="PC472_004102"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            305..376
                     /locus_tag="PC472_004103"
     tRNA            305..376
                     /locus_tag="PC472_004103"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:337..339,aa:Asn,seq:gtt)
BASE COUNT          120 a           60 c           80 g          118 t
ORIGIN      
        1 atttatttga tgttattcat taagatatat gtgtagtttt tagagtgtta actctatgta
       61 aagtactaac ttcaattgat gaagtatata tcgtaaaaac aattactatt tagtacttca
      121 aaactattga catttaaaaa taaagatgtt aatataatac ttgtcttata aataggcaat
      181 atgtggttat aatagcaaag aggatacacc tgttcccatt ccgaacacag aagttaagct
      241 ctttagcgct gatggtactt ggtgggaaac tgcctgggag agtaggacgt agccacgtga
      301 atattccaag gtagctcaat ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
      361 agccccaccc ttggagcc
//
LOCUS       DAMJYC010000275          344 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJYC010000275 DAMJYC010000000
VERSION     DAMJYC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.285"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>344
                     /locus_tag="PC472_004104"
     CDS             <1..>344
                     /locus_tag="PC472_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729322.1"
                     /translation="AFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGI
                     LVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLG
                     YTLKGVGDNSQM"
BASE COUNT           97 a           53 c           55 g          139 t
ORIGIN      
        1 gcattttctc ttactaatgg tatttctcct gtagtagcaa ctttaatggc tatcttatgt
       61 ggttgtactg ctggtcttgt tacagggatt cttcacatta agtttaagat atctaactta
      121 ctttctggta ttttagttat gggaatgcta tactctatca atttaagaat aatgggaaaa
      181 tcaaatattc ctctatttag ctttaaacac ttattcaatg gggaaatatc tcctatagtt
      241 ttagccttag cttttgtatt catctgtaaa gtccttttag atttattcct aaaaacaggt
      301 cttggataca ctttaaaagg tgttggtgat aactctcaaa tgat
//
LOCUS       DAMJYC010000276          326 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJYC010000276 DAMJYC010000000
VERSION     DAMJYC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.286"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>326
                     /locus_tag="PC472_004105"
     CDS             <1..>326
                     /locus_tag="PC472_004105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729323.1"
                     /translation="NPDTGKTQAKPEEKKPEAESKDIAEDTLNMFKEIKQIYHIYKFK
                     ADFNKDIIKKFKDPVEYIYVYENEMKKLKYEPEKLKEYCKTNPKINELREIHKILKEE
                     RKKYDE"
BASE COUNT          162 a           31 c           58 g           75 t
ORIGIN      
        1 caatccggat acaggaaaaa cgcaagctaa acctgaagag aaaaagccag aagcagaaag
       61 taaagatata gcagaagata cattaaatat gtttaaggaa attaagcaga tttatcatat
      121 atataagttc aaagcagact ttaataagga tataataaag aaatttaaag atcctgtaga
      181 gtacatatat gtatacgaaa atgaaatgaa aaagcttaag tatgagcctg agaagttaaa
      241 agagtattgt aaaacaaatc ctaaaattaa tgagttaagg gaaatacata agatattaaa
      301 agaagaacgt aaaaaatatg atgaaa
//
LOCUS       DAMJYC010000277          315 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJYC010000277 DAMJYC010000000
VERSION     DAMJYC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.288"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..268)
                     /locus_tag="PC472_004106"
     rRNA            complement(<1..268)
                     /locus_tag="PC472_004106"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           82 c           53 g          103 t
ORIGIN      
        1 ggctaatccg cgttcgctcg ccgctactta cggaatcgaa tttctttctc ttccttcagg
       61 tacttagatg tttcagttcc ctgagttccc ctcattaagc tatgtattca cttaatgata
      121 cttagacatt actctaagtg agtttcctca ttcggaaatc ttcggatcaa agtttacgtg
      181 caactccccg aagcttatcg cagcttatca cgtccttcat cggctcctag tgccaaggca
      241 tccgccctgc acccttaata acttgaccag ttaaaaaggt ttgatagatt agaagctatc
      301 aacttcgata atttt
//
LOCUS       DAMJYC010000278          311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMJYC010000278 DAMJYC010000000
VERSION     DAMJYC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.289"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
BASE COUNT          135 a           22 c           59 g           95 t
ORIGIN      
        1 tacattatta cataaattaa aacaggacaa tgaagaaaaa tttcaagagt atgcagagat
       61 attgagaggt attcttagag aacaagaaga gagaaaaaat aagtaaataa atagataaag
      121 cacttggata ttatgatgtt tcaggtgctt tgtttgtaaa aaaatggtat aatggaaata
      181 gagaattata ttaacaatgt ggtatgtaaa taatgaattt tgagaatcta cataggattg
      241 aactactaat tttatattaa ctatgtggta tgtaaatact aaattagagg tcgagaaggc
      301 tattgaatca a
//
LOCUS       DAMJYC010000279          276 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMJYC010000279 DAMJYC010000000
VERSION     DAMJYC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.291"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..>276)
                     /locus_tag="PC472_004107"
     CDS             complement(<1..>276)
                     /locus_tag="PC472_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729324.1"
                     /translation="NDRHRPGGTQYQGGIGRVSTENGSFGKGCSAKDSTGEGGGGGWF
                     GGAGMNGAGAGGGGSGYVLTKDSYKPTGYTPTSEYYFDNVVMESGGNT"
BASE COUNT           72 a           85 c           40 g           79 t
ORIGIN      
        1 gtatttccac cagattccat aacaacatta tcaaaataat attcagatgt tggtgtgtag
       61 ccagtaggct tataactatc tttagttaat acgtagccac ttccacctcc acctgctccc
      121 gcaccattca ttcctgcacc accaaaccag ccacctccac cgccttcgcc agttgaatct
      181 ttagcagaac acccttttcc aaaacttccg ttttctgtgc ttacacgacc aataccacct
      241 tgatattgag taccgccggg acgatgtctg tcgtta
//
LOCUS       DAMJYC010000280          257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMJYC010000280 DAMJYC010000000
VERSION     DAMJYC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.292"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..>257
                     /gene="tuf"
                     /locus_tag="PC472_004108"
     CDS             <1..>257
                     /gene="tuf"
                     /locus_tag="PC472_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2729325.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEV"
BASE COUNT          117 a           31 c           68 g           41 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagt
//
LOCUS       DAMJYC010000281          251 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMJYC010000281 DAMJYC010000000
VERSION     DAMJYC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.293"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
BASE COUNT          101 a           26 c           33 g           91 t
ORIGIN      
        1 tatattatct ttttaatatt gaacattata aatttactat ttataattta aaattataag
       61 gaggatatta ttatgaacag aaataatttt tcacatgaaa taattgaacc tattgataat
      121 ttagatgtta atttcaaact atttgattaa aaaatagcta cgcacactcg tgactttagt
      181 catgagttag tagttaaaat agtcagcata tagggaaacc tatatgtaga gataggatag
      241 aacctatcca a
//
LOCUS       DAMJYC010000282          219 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMJYC010000282 DAMJYC010000000
VERSION     DAMJYC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.denovo.294"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..>219)
                     /locus_tag="PC472_004109"
     rRNA            complement(<1..>219)
                     /locus_tag="PC472_004109"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           42 a           64 c           47 g           66 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtccc
//
LOCUS       DAMJYC010000283        57294 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.014, whole genome shotgun sequence.
ACCESSION   DAMJYC010000283 DAMJYC010000000
VERSION     DAMJYC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.014"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            78..2015
                     /gene="htpG"
                     /locus_tag="PC472_004110"
     CDS             78..2015
                     /gene="htpG"
                     /locus_tag="PC472_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2729326.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKHYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLFIEL"
     gene            2403..3533
                     /locus_tag="PC472_004111"
     CDS             2403..3533
                     /locus_tag="PC472_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2729327.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNSGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            3760..4782
                     /gene="splB"
                     /locus_tag="PC472_004112"
     CDS             3760..4782
                     /gene="splB"
                     /locus_tag="PC472_004112"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2729328.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            4964..5875
                     /locus_tag="PC472_004113"
     CDS             4964..5875
                     /locus_tag="PC472_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2729329.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            5890..6306
                     /locus_tag="PC472_004114"
     CDS             5890..6306
                     /locus_tag="PC472_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2729330.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(6406..6798)
                     /locus_tag="PC472_004115"
     CDS             complement(6406..6798)
                     /locus_tag="PC472_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729331.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            6939..7334
                     /locus_tag="PC472_004116"
     CDS             6939..7334
                     /locus_tag="PC472_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2729332.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            7737..7943
                     /locus_tag="PC472_004117"
     CDS             7737..7943
                     /locus_tag="PC472_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729333.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(8100..8831)
                     /locus_tag="PC472_004118"
     CDS             complement(8100..8831)
                     /locus_tag="PC472_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729334.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            9343..9657
                     /locus_tag="PC472_004119"
     CDS             9343..9657
                     /locus_tag="PC472_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2729335.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            9673..10059
                     /locus_tag="PC472_004120"
     CDS             9673..10059
                     /locus_tag="PC472_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2729336.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            10088..11473
                     /locus_tag="PC472_004121"
     CDS             10088..11473
                     /locus_tag="PC472_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2729337.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            11476..11628
                     /locus_tag="PC472_004122"
     CDS             11476..11628
                     /locus_tag="PC472_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2729338.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            11651..12856
                     /locus_tag="PC472_004123"
     CDS             11651..12856
                     /locus_tag="PC472_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2729339.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            12899..13120
                     /locus_tag="PC472_004124"
     CDS             12899..13120
                     /locus_tag="PC472_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729340.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            13237..13734
                     /locus_tag="PC472_004125"
     CDS             13237..13734
                     /locus_tag="PC472_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2729341.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            13709..14215
                     /locus_tag="PC472_004126"
     CDS             13709..14215
                     /locus_tag="PC472_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2729342.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            14199..16646
                     /locus_tag="PC472_004127"
     CDS             14199..16646
                     /locus_tag="PC472_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2729343.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            16649..18826
                     /locus_tag="PC472_004128"
     CDS             16649..18826
                     /locus_tag="PC472_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2729344.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            18823..19824
                     /locus_tag="PC472_004129"
     CDS             18823..19824
                     /locus_tag="PC472_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2729345.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            19821..20753
                     /locus_tag="PC472_004130"
     CDS             19821..20753
                     /locus_tag="PC472_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2729346.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            20998..21114
                     /locus_tag="PC472_004131"
     CDS             20998..21114
                     /locus_tag="PC472_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDF2729347.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            21130..23049
                     /gene="tet(M)"
                     /locus_tag="PC472_004132"
     CDS             21130..23049
                     /gene="tet(M)"
                     /locus_tag="PC472_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDF2729348.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            23168..23335
                     /locus_tag="PC472_004133"
     CDS             23168..23335
                     /locus_tag="PC472_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2729349.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(23395..23748)
                     /locus_tag="PC472_004134"
     CDS             complement(23395..23748)
                     /locus_tag="PC472_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2729350.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            24253..24675
                     /locus_tag="PC472_004135"
     CDS             24253..24675
                     /locus_tag="PC472_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2729351.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            24672..24902
                     /locus_tag="PC472_004136"
     CDS             24672..24902
                     /locus_tag="PC472_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2729352.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            25363..25566
                     /locus_tag="PC472_004137"
     CDS             25363..25566
                     /locus_tag="PC472_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2729353.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            25648..26865
                     /locus_tag="PC472_004138"
     CDS             25648..26865
                     /locus_tag="PC472_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2729354.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(27708..28451)
                     /locus_tag="PC472_004139"
     CDS             complement(27708..28451)
                     /locus_tag="PC472_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2729355.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            28617..31184
                     /locus_tag="PC472_004140"
     CDS             28617..31184
                     /locus_tag="PC472_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2729356.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            31414..31818
                     /locus_tag="PC472_004141"
     CDS             31414..31818
                     /locus_tag="PC472_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2729357.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            31842..32339
                     /locus_tag="PC472_004142"
     CDS             31842..32339
                     /locus_tag="PC472_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2729358.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            32342..32743
                     /locus_tag="PC472_004143"
     CDS             32342..32743
                     /locus_tag="PC472_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2729359.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            32761..33249
                     /locus_tag="PC472_004144"
     CDS             32761..33249
                     /locus_tag="PC472_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2729360.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            33276..34031
                     /locus_tag="PC472_004145"
     CDS             33276..34031
                     /locus_tag="PC472_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2729361.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            34031..34894
                     /locus_tag="PC472_004146"
     CDS             34031..34894
                     /locus_tag="PC472_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2729362.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            34941..35420
                     /locus_tag="PC472_004147"
     CDS             34941..35420
                     /locus_tag="PC472_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2729363.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            35485..36792
                     /locus_tag="PC472_004148"
     CDS             35485..36792
                     /locus_tag="PC472_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2729364.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            37102..37242
                     /locus_tag="PC472_004149"
     CDS             37102..37242
                     /locus_tag="PC472_004149"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2729365.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            37752..38846
                     /locus_tag="PC472_004150"
     CDS             37752..38846
                     /locus_tag="PC472_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729366.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            38851..39051
                     /locus_tag="PC472_004151"
     CDS             38851..39051
                     /locus_tag="PC472_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2729367.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            39051..39968
                     /locus_tag="PC472_004152"
     CDS             39051..39968
                     /locus_tag="PC472_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729368.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            39965..40723
                     /locus_tag="PC472_004153"
     CDS             39965..40723
                     /locus_tag="PC472_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729369.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            40971..42044
                     /locus_tag="PC472_004154"
     CDS             40971..42044
                     /locus_tag="PC472_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2729370.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            42114..42377
                     /locus_tag="PC472_004155"
     CDS             42114..42377
                     /locus_tag="PC472_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729371.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            42583..43560
                     /gene="bioB"
                     /locus_tag="PC472_004156"
     CDS             42583..43560
                     /gene="bioB"
                     /locus_tag="PC472_004156"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2729372.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            43789..44802
                     /locus_tag="PC472_004157"
     CDS             43789..44802
                     /locus_tag="PC472_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2729373.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            44844..45734
                     /gene="rbsK"
                     /locus_tag="PC472_004158"
     CDS             44844..45734
                     /gene="rbsK"
                     /locus_tag="PC472_004158"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2729374.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            45782..46744
                     /locus_tag="PC472_004159"
     CDS             45782..46744
                     /locus_tag="PC472_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2729375.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            46798..48309
                     /locus_tag="PC472_004160"
     CDS             46798..48309
                     /locus_tag="PC472_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2729376.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            48293..49291
                     /locus_tag="PC472_004161"
     CDS             48293..49291
                     /locus_tag="PC472_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2729377.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            49475..50737
                     /locus_tag="PC472_004162"
     CDS             49475..50737
                     /locus_tag="PC472_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2729378.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            50757..51500
                     /locus_tag="PC472_004163"
     CDS             50757..51500
                     /locus_tag="PC472_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2729379.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            51673..53058
                     /locus_tag="PC472_004164"
     CDS             51673..53058
                     /locus_tag="PC472_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2729380.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            53277..54116
                     /locus_tag="PC472_004165"
     CDS             53277..54116
                     /locus_tag="PC472_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729381.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            54106..54549
                     /locus_tag="PC472_004166"
     CDS             54106..54549
                     /locus_tag="PC472_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729382.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            54560..55051
                     /locus_tag="PC472_004167"
     CDS             54560..55051
                     /locus_tag="PC472_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729383.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(55253..55981)
                     /locus_tag="PC472_004168"
     CDS             complement(55253..55981)
                     /locus_tag="PC472_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2729384.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            56106..57194
                     /locus_tag="PC472_004169"
     CDS             56106..57194
                     /locus_tag="PC472_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729385.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
BASE COUNT        21317 a         7252 c        10764 g        17961 t
ORIGIN      
        1 ataatatgat tataatatat tgacatacca ttttaaaaaa tttaaaatat taattataaa
       61 atgaggaggg ataaaaaatg gaatttgaaa aagggagtat ttcaattcat acagaaaata
      121 tttttcctat aattaaaaaa tggttatact ctgataaaga tatatttata agagagctta
      181 taagtaatgg atgtgatgca gtaagtaaac ataaaagact tgtatcacta ggagaaatat
      241 cagaaaataa gtcatcagat tataaaatta cagtatctgt aaataaagga gaaggtacac
      301 ttaaatttat tgacaatgga attggtatga ctgaagaaga gataaaaaaa tacataaatc
      361 aagttgcttt ttcaggtgct gaagatttct ttaataaata taaggataag atggaagagt
      421 caaatgacat aataggtcat tttggattag gattttattc tgcatttatg gtgtctaaaa
      481 aggtacaaat agataccctt tcatatacag aaggtgcaac tcctgtaaga tggatatctg
      541 aaggtggtac agaatacgaa atatctgaat cagatactag aaatgataga ggaactacta
      601 taacactatt tattgatgat gatagtaaag aatttttaga tgaatttact gtaagaggaa
      661 taataaataa atactgttct ttcttaccag tagaaatata tttagaagat gttgaaagat
      721 tagaaagaga agctaaagaa gcagaagaaa aagctaaaaa acaaaaggaa gatggaaaag
      781 aagagattgt agatgcaaaa gtaattgagc ctttaaacga cactaatcct ctatggttaa
      841 aatctccaaa agactgtact gatgaggagt ataaagagtt ttatagaaag gtattcaatg
      901 tatttgatga acctttattt tggatacatt taaatgtaga ttatcctttc aatttaaagg
      961 gtatacttta cttccctaaa ttaaaaaatg agtttgaact gactgaagga aaagtaaaat
     1021 tatacaacaa tcaagtgttt gtagctgaca atataaaaga agttatacca gaatttttac
     1081 ttttattaaa aggtgttata gactgcccag acttaccatt aaatgtatca agaagtttct
     1141 tacaaaatga cagagacgta agcaaaattt ctaagcatat aattaaaaaa gtagctgata
     1201 agttaaaatc tctttgtaaa aatgaaagag aagaatacaa taagttttgg gatgatatac
     1261 aaatatttat taaatatggt tgcttaaaag atgaaagttt ctatgagaag gtaaaagaat
     1321 gtatattatt caaaactatt gatgatgagt atataacatt acaagattat ttagaaaaat
     1381 gtaaggacaa acatgaaaat aaggtatttt atgtaagtga taaggaacag caatctcaat
     1441 acattaaact atttaaagaa tacgacttaa gtgctgttgt tttaaactca tcaatagata
     1501 ctcattttat ttcatttatg gaatataaag agaatggagt taagtttaat agaatagatg
     1561 cagacctttc tgatgtatta aaagataaaa atgaaaataa agatagtgaa gaaaacaaag
     1621 aagagatagc taagatagaa ggtctgttta aagaagctgt tggagaaaga gtgaagcatt
     1681 attctgtaga aggattgaaa aatgaagaca ctccagctat ggtgttagta tcagaacaat
     1741 ctattagaat ggcagaaatg cagtcaagat ttgctggaat ggatttagga atgaattttg
     1801 aggaagaaaa aactcttgta ataaatgaga acagtccaat aatcaaaaaa ttggtttctc
     1861 ttaaagatga tgaggaaaag aaagataaga ttacgcttat ttgtaaccag atagcagatt
     1921 tagctttact ttctaataaa gaattaaagc cagatgagtt agattcattt gtacaaagaa
     1981 gtaataagct tatgagtctt ttcatagaat tataagattg aatttgaata cattgaagca
     2041 aatttataaa aagtattcaa tatcagtgat gataaagaag tgtctcaaaa tgaacttttc
     2101 agttcatggg gcgctttttt tatgtgaaat taaatttatt ttaatcattt caacaatgaa
     2161 aaatatattt atctctattt tgagacagac cattctttat ttttttatct ttggttaatt
     2221 tttaaatatt taagtaggtt ctaataaaaa actctattta gttaaatata gttaacttga
     2281 tatataataa gataatacat ataattaagt aaaagattgt tttttagttt atgtttaaat
     2341 acataattac actttgttaa aaacataaaa ttattactaa agtagaaata ggaggtttta
     2401 gcatgaattt taattataat ttgccagtaa atatcttatt tgggagaggc agaattaatg
     2461 atgtgggaaa agaagtatct aaatatggca aaaaagtatt gatagtaaca ggtaaaaata
     2521 gtacaaagaa aactggatta cttgataaaa caatagattt actaaaagac tctaaaatag
     2581 aatatgaagt ttttgataga gtagaacaaa atcctcttat aactactata tattcaggag
     2641 tagaaattat aaaatcaact ggatgtgatt gtgttttagg gcttggtggt ggaagtatta
     2701 tggatgctgc caagagtata gctttttctt ataaaaattc aggagattta aatgattata
     2761 tatttggaat tagacaaggt gaagaagctt tacctataat cttagttcca acaacatcag
     2821 gtacaggtag tgaaggaaat tgttttgcag tacttacaaa ttcagaaact aaagataaaa
     2881 aatctataaa gaagaatagt atgtatgcaa aagcatcaat aattgaccca gaacttatgg
     2941 ttacaatgcc aaaacatata attgcatcag ttggatttga tgcacttgcc cataatatgg
     3001 aagcatatct ttctaatgga agaaaccctc ttgcagatgt acaagctatt tatgggattg
     3061 aacttatatc agaaaaccta ataaaggtat ataatgatgt taatgattta gaagcatggg
     3121 aaaagataac acttgcaagt actattggag gaatgacgat tggtacagcg ggtacagcct
     3181 tgccacatgg aatggagcat cctgtaagtg gtcttagaga tgtaatgcat ggaaaaggac
     3241 ttgcagcttt gactcctact attatagaaa gttcatggga tagtgatatt gaaagatttg
     3301 gtaatatttc aaaggtatta ggaggaactt gtgcagaaga ttgttcagat gcaattagaa
     3361 atttccttaa aaaaatagat ttagatataa agctaagtga tttaggtatt gaaaaatcag
     3421 atatagattg gttaactgat aattgtatga aaatatcagt tgcaaatatt aagagacatc
     3481 caaagtattt taataaggaa caaataaagg aaatgtacca taagtcttta taatttgact
     3541 ttacatattt agttttatat aaaaattata aattaataat aaaaaattat aagtaattaa
     3601 tagtaaaaaa attgtgtata gaagttatat atataataaa tttgtcttaa gcttttattt
     3661 tactttctaa taaataaagg agtttaaaaa agggaagatt aattaagtaa tattttataa
     3721 aattcttcga tttaaaaact ttaagtagag gtgaaataaa tgtttaatcc caaaagaata
     3781 atattcgaaa aaggaacttt agacactgat gtgggtaaaa aaatatacaa taaatttaga
     3841 gataataaag atgtagagat aattaatata tcatcaaata ggattaaaga acatattcca
     3901 ggtgaaaatc tatatgaaca atatagaagt gggaagcaga ctatggtagt tgggattaga
     3961 aaaagtctga agtttcaaac atgtaaacct tctgctaatt atcaactacc acttgtcagt
     4021 ggatgtatgg gaagatgtga gtattgttat ttgaacacac aattaggtga taaaccattt
     4081 ataagggtat ttgtaaatgt agatgaaata cttgaacaga caaaaaaata tattgaagat
     4141 agaaagccac aggtaaccat atttgaaggg gcagctacat cagacccagt tccagtagag
     4201 ccatatacta attctttgaa gaaaacaata gagttttttg gaagagagga aaaaggaaga
     4261 tttagatttg ttactaaata cagtgatgtg gatagcttat tggatgctga acataataat
     4321 catacagaaa tacgatttag tatcaatact cctagaatta taaatgaaca tgagcattat
     4381 acagcctctg caaataaaag aatagagtca gctgtaaaac ttgctagggc tggttatcag
     4441 attggtttca taatagcacc tgtgttttta tatgaaggtt ggaaagaaga gtataaaaat
     4501 ctgattataa gtataaaaga agctttacca aaggattttg ataaaaaaat tatatttgaa
     4561 gttatatctc atagatatac aactaaagca aaaaatagaa tactagagat ttatccagat
     4621 acactattgc caatggaaaa tgaagataga aagtttaaat acggtcaatt tggatatggt
     4681 aagtacgtgt atgataaaga acaattagat gatatgaaag agttctttct taaggaaata
     4741 aatggagtat ttaaaaatag tttaataaaa tatataatat agcaaatctt acctaaagat
     4801 ttgctatatt ttatttttat atattttatg tttataataa agcaatgaga tttagcacta
     4861 tcatataaga aaattaaaaa gggcactatc tatcaagaac attttttcta gttttgatac
     4921 tataaaggat aagagtaaca aaaatatcag gaggagttat agtatgcatg catgggagtc
     4981 tattcaaatt acattggatt taatagaagc aaatttatca gaagaaatat caattaatga
     5041 attagcaaat aaagcaaatt tatcaccatt ttactatcaa agactattta aaagattggt
     5101 aaataagact gtaatggagt atataaagct tcgtcgtctt gcaagagctt ctgaatactt
     5161 agcagaacat aagaatcgta tacttgatgt agctttaaat tttggatttg ctagtcatga
     5221 gacatttaca cgttccttta aaaaagcata tggattaact ccagaaaaat atagagcaaa
     5281 tccagtaaaa ctcaatcact ttattaagcc agaattaatt ttaaattatg ctatggttga
     5341 tgaaggtgtt ccactgattg cagatgatat agtgattgaa gtcaatcgta agacattgag
     5401 taatccaagg acatttgttg gaatagaaat agaagtacct atttgccagt tggtgggcgg
     5461 tgaaacaact ggaattgcaa tagtagagga attatggatg aaacttggaa gtcaaagaca
     5521 taatatacca aatcaaattc caggtggaaa tgaatttgct gctctttata tgggaaatgc
     5581 aaaagaagga aattgtatgt atatggcagg tgtagaggta gagtcaggta catcagtaga
     5641 aggatatagt acttttgagt tgccagctaa agaatattta gtatgtggat ttgaggcaga
     5701 aaactttaat gagttagtta attcagctgt ttttaaagca gataaattta tggaaagatg
     5761 gatgaagaaa cataatttaa caactactga ttttgcaatt gagatgtatt accccactac
     5821 acctgaagca gcatatctgg aacattggat tgttccggtt cctattgaac aatagaaagg
     5881 atgatgaata tgaattggca agatgcgttt tttcaagata ctaaacctac atttgagcaa
     5941 attaatgaat ttattaataa tccattatgg gacaagttaa ataaaacttt ggtttctact
     6001 tataatgtag aacctaagct tgaatacagt aaatgttcaa tgcaaagagg ttggaatgta
     6061 aaatataaaa aaagaggtaa atcactatgt actttatatc cacaagaagg ttattttaaa
     6121 gctttggtta ttgtaagtga aagcaataga gtagaggttg atttatttat taatacttgt
     6181 tgtgattata tcaaaaaaat atataatgag gtaaactttt ttaatggaag taaatggcta
     6241 atgattcaag tagatagttt attggtatta aacgatatgc ttgaactaat taaatttaga
     6301 gcttaacata tagaacagga tgaatatata agtcatcctg tttttatatt caatcagata
     6361 aattaatata tagtctataa tatgaataat ttagtaaatt taaatttact ttatctttct
     6421 acattctttg caactaagtc cacatgtgtt tttttcttgc atattagtag caccccaatt
     6481 agcaaattgt tgtaaaatag gcacaatact tttaccttta cttgttaatg tatattcaac
     6541 ttttggagga atttcattat attgtttacg atgaacaata ccagtagact ctaaatcttt
     6601 taatgattga gctagcatag tatttgtaat gccatccaat tttcttttta tgctactata
     6661 tctaattgga ttttcaactg ctaacataca tataataggt aatttccact taccaccgac
     6721 aatgtctaat gcataacgga ttggacaggt taattcttca acgtttttag ttatattttt
     6781 ttcagtattt atactcattt taattactcc ttagttatag tagtatcttt tttatacttt
     6841 atcagaaaaa actgcatact tgttttttct gtattttata ttaaaattat atcaaaggat
     6901 aatccttaat acaagtattt aaaaggggtg taagaattat gaaagcatta gaatttttga
     6961 acaaggcacg agtttattat ttagcaacag ttgatgagaa caatcaagca catgtgcgtc
     7021 ctttaggatt tgttatggaa tacaatggta aacttacatt ttgcacatct aatcaaaagg
     7081 atatgtttaa gcaacttatt tttaatccaa aggttgaact ttgttgtata gaccaaaacc
     7141 ttaatactct acgtatttta ggtgaagcag tatttgttac atcaaaagaa acacaagcta
     7201 aggcacttga aataatgcca agtttaggtc agatgtactc tgtaggagat ggtaagtttg
     7261 aaatattctc tattgataag gcaaaagtta gttgttgttc aatgagtggt aaaaagattg
     7321 atgtagagct ttaattataa ttgaatcgac atccatagtg aaaattaaga aaacgttatt
     7381 actgaaatgt tgatagattt aatttaaatg caataaaaag atttttaaat aatgaatatg
     7441 taaaaatttt tagttattag atgtgtattt caataatgat atactatttt gaataattac
     7501 atactagaaa taagataatt aaaatttact ttataatggg atagatttct tttaaaaaat
     7561 attttaacta taattgattt ttatttaatc tgaaaaaaat attatgacta taaaaaattg
     7621 ctacttatta tttggatatt tttatatata aataataagt agcatgactt tatacattaa
     7681 taatgataga atataaatta atagataaga tatgatttta aaaggagtga tataagatga
     7741 gtaaatatag ttctttatgg gaatatgtta agaaaaacaa tagtcaatcg tttaaattaa
     7801 cttttgaaga aataaaagat attgctggga tagagataga ccattcgttt ttgaaatata
     7861 aaaaggaatt aaatgaatat ggatatcaag taggaaaaat ctctttaaaa gagaagactg
     7921 ttattttcga taaaattggt taagacataa ataattacat ataataaaat aatttgtatt
     7981 gatgattatt acaaataaaa aagggcgtag cactattttt tagtgtcaca gccccttttt
     8041 tgtttataat tatgtacatt tcacattatc acgatataag catttttctt atgagaaaat
     8101 taattagaat tatttttatt catatttaaa ttatttaaat tgttttccca gttttttatc
     8161 tcctttttaa cttcttcact taaagtatca cttgaagata ttttctttaa tttatcattc
     8221 aaagatttct tttgttcctc tgaaaggtct gtttttattg taggatcaat aatatctatt
     8281 acatctttta catcaaaatc aacaaatttg cacagttctt ctagatgatc aattagaaaa
     8341 tctagacctt catatgacca ttctttataa tcttttccgt tttttaatag aaactcattc
     8401 acttggtaat tctgatttgc taattcaaag aatgaacctg aaggatccat tatatcattt
     8461 atagagtttt catagttaac aatattaatt tgtcttatta atttctcttt ttctgttaag
     8521 ttgctataat tcttgttaac aaaagctact aatccaaatt caaagtttaa aattgaatct
     8581 ttacgttttt ttactgaatc ttctccaaat tccctaagac cttctaaatt tttatcaatg
     8641 gctttatctc taatctctat taattgttct atacttaaac cttctaagtt tttaggtact
     8701 atttcttcat tattcttagt aacttctttc aatttattgt tagtattttt attattgaaa
     8761 ataaatatag cagttaataa acttaaaatt attactaaaa taattaaact tacatttttt
     8821 ttacttaaca tattaaacct ccttttttaa ataagtattg tacttgtgaa aaataaattt
     8881 gtttatgaag ataacattta ctactgtttg ttctaaagat tataatagaa tatttttttg
     8941 atataatcta tatttttctt attaaatttt cttattagat atttaatatc aatatttttt
     9001 agacataaaa atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt
     9061 atcaaggtca aaaccactca atttactact aatttactac ttatgaatga gctttgatac
     9121 gacgatttat ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta
     9181 ataggtagac acttcaaaaa atgaggtgtc tattttttta cccgattttg aaaggaagtg
     9241 aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc cccactcttt aagaccatca
     9301 aacattcatt cagccaataa aaaagaaagg ataggtaaaa atatggaact taaatttgtg
     9361 attcccaaca tggaaaaaac attcggcaat ttagaatttg ctggcgagga taaagtcgtt
     9421 cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa gctataatct ctattctgat
     9481 gttcaaagag cagatgatat tgtggtggtg cttcctgctg aagctggcga aaaacatttc
     9541 ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg ctacaaaatc
     9601 ggcactcgtg gttttacaaa ttacctttta catgctgacg acatgataaa agaataaaga
     9661 aagagaggaa aaatgatgag attagcaaat ggcattgtat tagataaaga cacgactttt
     9721 ggagaattga aattctctgc tctacgtcgt gaagtgagaa tccaaaatga agacgggtcg
     9781 gtttcagatg aaatcaagga acgtacctat gacttaaaat ccaaaggaca aggacgcatg
     9841 attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt ttgattataa cgcacgggtg
     9901 gaacttatca atcccattgc ggacaccgtt gctactgcca cctatcaagg agcagatgtt
     9961 gactggtata tcaaggcaga cgatattgtg ctgacaaagg attctagttc attcaaagct
    10021 caaccacaag caaagaaaga accgacacaa gacaaatagt cgctaggtag aaaggagact
    10081 ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt tagtctttca
    10141 ttttacgata gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt ttcatgtgaa
    10201 gacaatccag cagatcaact ggcaggattt taacctatca caagcagata agattgacat
    10261 tccctattta attatcagtt tcagtgtcgc aattcttatc tgcttgctgg tagcgtttgt
    10321 attcaaacgg gttcgctatg atacggttaa acaactttac caccgtcaaa aactggcaaa
    10381 gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg gtttctttaa
    10441 agatagtgct ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt attatcgact
    10501 taaaaatggc ttgatacaga tacgggtgga aatcacgctg ggaaaatatc aagaccaact
    10561 cttacacttg gaaaagaaat tagagagtgg cttgtactgt gagctgacgg ataaagagtt
    10621 aaaggattcc tatgtggaat atactttgct ctatgacacc atagccagtc gtatttctat
    10681 tgatgaagta gaagctaaag atggtaaact tcgcttaatg aaaaacgtat ggtgggaata
    10741 tgataagctc cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa cttactttat
    10801 actgacactg attgaagcct tgcttcatac agattcaaaa ctgtatattc ttgacccgaa
    10861 aaatgctgac cttgcggact taggttctgt gatggcaaat gtctactata gaaaagaaga
    10921 cttgctttct tgcattgaaa cattctatga agaaatgatg aaacgtagtg aggaaatgaa
    10981 gcagatgaag aactataaga ctggcaaaaa ttatgcttac ttaggtctcc cggcacactt
    11041 cttaatcttt gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag aaaacaccgc
    11101 agttatgaat aagctgaaac agattgtcat gttaggtcgt caagctggct tctttctaat
    11161 actggcttgt caacgtccag acgcaaaata tttaggcgac ggaatccgtg atcagtttaa
    11221 tttcagagtg gctttaggtc gtatgtctga aatgggctat ggcatgatgt ttggcagtga
    11281 cgtacaaaag gatttcttct taaagcgaat caaaggtcgt ggctatgttg atgtaggaac
    11341 aagtgtcata tcagagtttt atactcccct tgtaccaaaa ggatatgatt tcttggagga
    11401 aattaaaaag ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag cggaagtcgc
    11461 aggtgtggac tgatcttgct ggctggtgtg gcaatagcca cgccagcact taaccccccg
    11521 tatctaacag gggggtacaa atcgacagga aacagtcaaa aaaacattag aaaatccttt
    11581 ggttacaagg gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa gttgacatac
    11641 gcctttttga ttggagggat ttttactgaa tgaacaaact tggttacagc atttaaaaga
    11701 aaaacgcttg gcttatggac tatctcaaaa ccgtttagct gttgcgactg gtattacaag
    11761 gcagtatcta agcgatattg aaacaggaaa agtcaagcca tcagaggatt tacagcagtc
    11821 cctttgggaa gctctggaac gcttcaatcc cgacgctccc cttgaaatgc tgtttgatta
    11881 tgtaaggatt cgctttccga caacagacgt acagcaggtg gtcgaaaaca tcttacaact
    11941 gaaactgtcc tattttcttc atgaggacta tggtttctat tcttattcag agcattatgc
    12001 tttaggcgac atattcgtcc tttgctccca tgaactggac aaaggagttc tggtggaatt
    12061 gaaaggtcgt gggtgcagac aatttgaaag ctatcttctg gcacaacaaa gaagctggta
    12121 tgagttcttt atggacgttt tggtggctgg cggtgtgatg aaacgccttg accttgccat
    12181 taacgataag acagggattt tgaatatccc tgtactcact gaaaagtgcc aacaggaaga
    12241 atgtatctcc gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg tacgcaaaga
    12301 ggaaaaggaa tgtatgggaa acaccctcta tatcggttca ttacaaagtg aagtttattt
    12361 ctgtatctat gaaaaggact acgagcagta caagaaaaat gatattccca ttgaagacgc
    12421 agaagtaaaa aaccgttttg agattcgatt gaaaaatgag cgtgcctatt atgcagtccg
    12481 tgatttactc gtctatgaca atccagagca taccgccttt aaaattatca atcggtatat
    12541 ccgttttgta gataaagacg attccaaacc tcgttctgat tggaaactga atgaagaatg
    12601 ggcttggttt attgggaaca atcgtgaacg attaaaacta accacaaaac cagagcctta
    12661 ctccttccaa aggacgctga actggctatc tcatcaagtt gccccgacct taaaggttgc
    12721 gattaaactt gatgaaatca accagacgca ggttgtaaaa gacattctcg accatgcgaa
    12781 actgacagac cgacacaagc agattttgaa gcaacagtca gtaaaagaac aggacgtgat
    12841 aacaacaaaa aaataactca aatacaaatt cattgaatat agagaggaga acatttttat
    12901 gaattttgga caaaaccttt ataactggtt tctatcaaac gctcaatcac tggtgctttt
    12961 agcaatcgtt gtgattggct tgtatcttgg cttcaagcgt gagtttagca aactgattgg
    13021 ctttttaatt attgcgatta ttgcggttgg cttagtcttc aacgctgctg gagtaaaaga
    13081 cattttacta gagctattca atcgcattat tggtgcttaa ataaaaccgt tcttttgtgg
    13141 aatataagtg gttttcttat gttccgcaaa ggaatggtac accaaacgaa gtgcggtagg
    13201 gatttttgaa tctctacaaa gaaaggacgt gaatatatgg acgatatgca agtctatatt
    13261 gcgaatttag gcaaatacaa tgagggcgaa ttggtcggtg cgtggtttac ctttcccatt
    13321 gactttgagg aagtcaaaga gaaaatcggc ttgaatgatg aatatgagga atacgccatt
    13381 catgactacg agttaccctt tacggttgac gaatacactt ccattggcga actcaatcga
    13441 ctatgggaaa tggtatcgga attacccgaa gaattacaat cggagctatc tgctctgctc
    13501 actcattttt caagcattga agaactaagc gaacatcaag aggatattat cattcattcc
    13561 gattgtgatg atatgtatga cgtggcacgc tactacattg aagaaacggg tgctttaggc
    13621 gaagtaccag ctagtcttca aaactatatt gattatcaag cctatggtcg ggatttagac
    13681 ctttcaggaa cgtttatctc aaccaatcat gggatttttg aaatcgtcta ttaaatctgt
    13741 cggtacatta ctactggcag attttctatt ttacggggtg gctcaatcag ctacccctat
    13801 tttttatgaa aggattgatt acatgaagaa aatacgaagc tataccagta tctggtctgt
    13861 ggaaaaggta ctgtattcta tcaatgattt tagacttccg tttcccataa cctttacgca
    13921 aatgacatgg tttgtcgtgt cactctttgc agtgatgata cttggcaact tgccccctct
    13981 ttccatgata gagggagcat ttctcaaata ctttgggatt cctgtggctt tcacatggtt
    14041 tatgtctaca aaaacttttg atggtaaaaa gccttatgga tttttgaagt ctgtcattgc
    14101 ttatgcactg cgaccaaagc tgacctatgc aggaaaaaaa gtaacgcttg gcagaaacca
    14161 gccacaagaa gccattacag cagttaggag tgaattttat ggcatatcca attaaataca
    14221 ttgaaaacaa tctcgtctgg aataaagacg gggaatgtta tgcttactat gagcttgttc
    14281 cttacaatta ctcatttcta agtccagaac agaaaataca agtgcatgat tctttcagac
    14341 agcttatcgc acaaaatcgt gatggcaaaa ttcatgcttt acaaatcagt acagaatcca
    14401 gcatacgttc tgcacaagag cgttccaaaa atgaagtcac tggcaagctc aaagcggttg
    14461 cctatgacaa aatcgaccaa cagacagacg ctttaatatc catgattggc gaaaatcaag
    14521 tgaactaccg tttctttatc ggctttaagt tgcttctcaa cgatcaggag ttttctatga
    14581 aaagtcttac cgttgaagca aaaaatgctt tgtctgattt tgtctatgat gtgaaccata
    14641 agctgatggg cgattttgtt agtatgagta atgatgaaat cctgcgtttt cagaagatgg
    14701 aaaagctctt agaaaataaa atctctcgtc gtttcaaaat ccgcaggtta gataaggacg
    14761 acttcggcta tctgattgaa cacctttacg gacagacagg cactgcctat gaagagtatg
    14821 agtaccatct atcaaagaaa aagctggata atgaaacgct gattaaatac tatgacttga
    14881 ttaagcctac tcgctgtttg gtggaagaaa aacagcgata tttgaaaatc cagcaggaag
    14941 atgaaaccgt ctatgtagct tactttacca ttaacagcat tgtcggagaa ctggacttcc
    15001 cgtcctctga aatcttctac taccagcaac agcaatttac attcccgatt gatacgtcaa
    15061 tgaatgtgga aattgtagcg aatcgtaaag ccctatctac tgtccgcaat aaaaagaaag
    15121 aactgaaaga cttggataac cacgcttggc aaagtgataa tgaaaccagc tccaatgtgg
    15181 cggaagctct ggaaagtgtg aatgagctgg aaaccaattt agaccaaagc aaggaatcta
    15241 tgtacaagct gtcttatgtg gtaagggtat cagcaaatga tcttgacgaa ctcaaacgtc
    15301 gttgtaatga agtgaaagat ttttatgacg atttaagcgt aaaactggta cgaccatttg
    15361 gggatatgct cggcttacat gaagaatttt tacctgccag caagcgttat atgaatgatt
    15421 atattcaata cgtgacctct gatttcctcg ctggtttagg ttttggtgct actcaaatgc
    15481 tgggggaaaa tgaggggatt tatgttggct acagcttaga tactggacgc aatgtctatc
    15541 tgaaacctgc tcttgccagt caaggggtta agggttcagt aaccaatgcg ttagcgtcgg
    15601 cttttgttgg ttcgctgggt ggtggtaaat cctttgcgaa taaccttatc gtctattatg
    15661 cggtgcttta tggggcacaa gcagtgattg tagacccaaa agcagaacgt ggcagatgga
    15721 aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact tctgatgaga
    15781 aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat tctgaatcac
    15841 tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg gaacgcttcc
    15901 caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg ttgatgaaag
    15961 tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc gaccatatcg
    16021 aaagttttac agactatgac tttgcacatt tattattcag taatggttat gtggagcagt
    16081 ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta cttcccgaca
    16141 aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct atgctgattg
    16201 tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc aagattgtag
    16261 atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta tctatgaagc
    16321 tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa aatacagacg
    16381 acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt cgttccactg
    16441 accttaacga gattaaaaag accttagcct tttttggtgt agacccagag gacgaaaaca
    16501 atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat ttatatggtc
    16561 gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc tttgatacca
    16621 gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca gaataaaatc
    16681 aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca cacttgtgat
    16741 tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct tggtagatga
    16801 tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc aactggattt
    16861 ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga ttggaaaaca
    16921 ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt tgtatgtttc
    16981 caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt ccgctacagc
    17041 agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg gattttcaac
    17101 agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg tttatgttgc
    17161 ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta tgaattttgt
    17221 gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca ttaaaaaaat
    17281 caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga agattgtcat
    17341 gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata gcctgttttc
    17401 catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca ttgaaagtat
    17461 cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca atggcgaaga
    17521 cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc taaccattac
    17581 aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata ttgggatttc
    17641 catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt ttatcatcta
    17701 tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg atggtatgtc
    17761 aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa tcacattgat
    17821 tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg gttatccgtt
    17881 ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca agctgggcga
    17941 tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa gtcgtgtgat
    18001 gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga aacttggacg
    18061 ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag gacagtcggg
    18121 ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa aggaaaaatc
    18181 aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca aagacagaat
    18241 ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca atgcaagata
    18301 tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa ccagtagtat
    18361 ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac aaaggcgaaa
    18421 aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac agcctgcttc
    18481 ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac agacctcaaa
    18541 acagtctaat attcagactt catataagga atctcaacaa gccaaacaag agcgtccagc
    18601 agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg ttcaagaaaa
    18661 aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac aacgtccaat
    18721 cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa gaagtagacc
    18781 accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt gaaaacttta
    18841 gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt tgttgccatt
    18901 ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg tgtgaatgtt
    18961 tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga atatggcgtt
    19021 gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg tactgcggaa
    19081 gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag tacagaagaa
    19141 tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga aaggctcagt
    19201 gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt agggtatgtg
    19261 gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa agagtattca
    19321 ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg gggctggcga
    19381 tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt cacaacagag
    19441 tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga gggctggcga
    19501 tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact gacacaatgg
    19561 acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata tgatgtgacc
    19621 cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca ttctacctat
    19681 aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg tatgtttcat
    19741 gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca gcatttagtg
    19801 ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt agaaaaaatc
    19861 agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag gtcaatcctc
    19921 ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc tttgcgatat
    19981 tcaagcactt tacagctata gatactcata ctattcacga aacaactatc atagaaaagg
    20041 aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa gtctactatt
    20101 catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa ggctatctga
    20161 cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct gtatcgtctt
    20221 ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag tttaatgtaa
    20281 cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc cactctgctt
    20341 atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag aatccgacca
    20401 ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt gaggggacgg
    20461 ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc aagctctatc
    20521 ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta aaaccaatcg
    20581 gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag gataatcaag
    20641 tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg caggtatctc
    20701 aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat
    20761 tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt
    20821 cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa
    20881 atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca aataattgga
    20941 tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg
    21001 attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat
    21061 cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt ttgaatggag
    21121 gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact
    21181 accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac
    21241 aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag
    21301 acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga
    21361 catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta
    21421 ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg
    21481 aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta
    21541 tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag
    21601 gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca atgggatacg
    21661 gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca
    21721 ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat
    21781 catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa
    21841 ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa
    21901 tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta
    21961 cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca
    22021 ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa
    22081 aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa
    22141 aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag
    22201 agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat
    22261 tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
    22321 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
    22381 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
    22441 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
    22501 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt
    22561 atggaaggga tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt gacggactgt
    22621 aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg
    22681 atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag
    22741 ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat
    22801 gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt
    22861 agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca
    22921 aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct
    22981 gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa
    23041 ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat
    23101 attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta taaactacaa
    23161 gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg aagatactga
    23221 attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa
    23281 gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc gataaaatga
    23341 gattaataca atctcatttt atcggctctt tccgttatgt atggattctt ttaattagtc
    23401 ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg atagttcttt
    23461 gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt
    23521 attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa ggtcataaag
    23581 aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc gtgggtcaat
    23641 ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag cttctttaat
    23701 ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag tagaccacct
    23761 ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt gttatatggt
    23821 ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg
    23881 gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg tttgttggag
    23941 gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg gttcttgtca
    24001 aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt ataaatcata
    24061 tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg cttttttggc
    24121 tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc cctttcacat
    24181 tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct agacagaaag
    24241 ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca gtttgactat
    24301 atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct ttcaaggatt
    24361 gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca gtttgcgaca
    24421 acagataact attcaactga ctttcagatt tttacactca atgggttatc agtaggcgtt
    24481 gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg tgaaattcta
    24541 ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt gaaattgaac
    24601 cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa gtttatggag
    24661 gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt gtaaaggcaa
    24721 cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg tacataacga
    24781 agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc gttgatgaag
    24841 tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt gaaattaagt
    24901 aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc gaacagggag
    24961 gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc taacgagcca
    25021 ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa agagccaacg
    25081 gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta gtgatccgag
    25141 aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg atactcccat
    25201 acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc tacatgagaa
    25261 ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct ggtaaaacaa
    25321 cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca gactgacatt
    25381 cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt tcgtattggc
    25441 gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct gattatgaat
    25501 ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac attggacgca
    25561 atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt atcaaggctc
    25621 tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa taaaggtcga
    25681 atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa atatatagat
    25741 tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga ccgagtacca
    25801 gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca gaaagacatt
    25861 catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta cgcaaaacag
    25921 aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata tcttatggat
    25981 attttgaaga aagacaagtt aggtgtaaga agtattgaca gtattaagcc atcagacgct
    26041 aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat caataactac
    26101 aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt tcggaagaat
    26161 ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa gaccgtacta
    26221 acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac ctacagcaaa
    26281 aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga gtttggtggt
    26341 ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca tcagctattg
    26401 agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg cgaacgtcaa
    26461 gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa tcgaaagaat
    26521 gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag aaagaactat
    26581 ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa atacaataag
    26641 tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac attctgtacc
    26701 aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg acatgctaat
    26761 atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat ggcagaaatg
    26821 aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta caaatgaatt
    26881 tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa gaaacgtgaa
    26941 gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac gagcatttaa
    27001 gaaaatataa aaagataatt agaaatttat actttgttta gatagaaaag attttaataa
    27061 ctccaaatat ttaataagta aaataaaatt attgctttaa aatatatttt tatatgtttt
    27121 aagttttata ataagtaata ttgtatgata aattaaattt aaatatttag agataagcta
    27181 ttatagaatt tgcttagttt aagttaaatt ttaaaatatt caaaaagggg ctagagtctg
    27241 gaaattttta tttaaagaat acacataaaa atttctagac tctcatcatt taaaagaagt
    27301 attctaaatt tattatttat aattaaatat tatctgaaaa aatggtattt ttataccttt
    27361 gtttgttatg cataatttta cttagaacat aagctttata taaaataatt aaaaatatac
    27421 ctaattaaaa taagtaaaaa aagagctatc atagaacaaa tttaatttgc tcatgtgaca
    27481 gctccttttt agatgatgct tttttagatt tatactataa ggtatataaa actaagttta
    27541 ttaaaaatgt tataatttct attgctatca tagaccatat caatccatca agttcataaa
    27601 agtaattaag ctttattaaa tagcttcttt attataattt ctttatttca tataatataa
    27661 atagtattaa aatttttatg ggagttaaag taagcatgta ttattactta gatatttgta
    27721 taatcaacat ctagtaattt acctttttgg tgaaaatgat taataagttt tttaggataa
    27781 ttcattatta attcatctgg aaaattaatt tcctttaaga tattttcaga atactcatag
    27841 ttacctatgt caaaacaaat gtgagcatct gaagtcagta tgattttagc accatatttt
    27901 ttgcaaagtg tagcaatata cttacagtta tccgagctac catttcttga cacgccttta
    27961 atagaacaat tatttatttc tattaagata ttcttttcta tagctaattt aatgattaat
    28021 tcatagtcga ttgggtagtt tgggtttcca aggtgtccta atatttcaat attatcatgc
    28081 ttatttattg catttattaa agctttagta ttattctcta atgtatttgg agaaaacaca
    28141 gcttcgtgaa atgacaagat aagataatct aaccgaggaa ttacaaaagg ctctaggtct
    28201 atgtttcctt taatatctag tatatttgct tcacagcctc ttaaaattat aatgttattt
    28261 atgattcttg ggatattttg aatgttgtga aagtaccatt tgtggggaga accaggcatc
    28321 ttaggaccat gttcagatgt acctagtatc ttaattccat tttctttaca ataattaata
    28381 ttttctagta aagtgctata gccatgacca ctgactatag agtgtgtgtg taaatctgat
    28441 aaaaaattca tataaattcc tcctctataa aagatgaggg taacgtaatt tatattataa
    28501 aacgaatagg aaagaaaatc aatagttaca taattagaca aaaatattaa aatattgtag
    28561 aattaagctt aataataaat gtttaacagt attactgctt ataggggagg ttaactatga
    28621 cacaaaagaa gctatcaagg aaagaaaaag ttttaatttg cttgaaaagg ttaacagaag
    28681 aacacttgaa ctttaataat ggcattagat ttggatttga tgcagaatat atagctactg
    28741 aagtaggtat aaatagaagt aatgctagta aggaattaaa tcaattaata aaagaaagta
    28801 ttattataaa gataaagggt aagcctgttc agtacttata taaagaaagt attgagagta
    28861 ttttaggaaa gaaactaaat aaagggatat ttgataatat taactctata aacgaatata
    28921 gtgaaaatga ttctatcaaa gatgagtata ttttagagag tataaaaaaa gagaacaata
    28981 tttttaattt agtaggatgt gatggaagtt taaggtccca aatagaacag gctaaagcag
    29041 cagtaatata tccacctaag gggttgagta cattaataat aggacctaca ggagttggta
    29101 aatctatatt tgcagaatat atgtataagt atagtttaac tataaaagaa gatagtgata
    29161 atgcaccatt tatcatattt aactgtgctg attattctga taatcagcaa ttattattag
    29221 cgcagctttt tggatatgta aaaggagcat ttactggagc tgataaagat aagtatggta
    29281 ttgtacatga agctaataat ggagtattgt ttttagatga agttcataga ttatctgcag
    29341 agggacaaga aatgctattt ctgcttatgg ataaagggat atatagaagg ttaggagagg
    29401 ctaataaggt acacgagtgt agagttatga taattgcagc cactacagaa gacccagaaa
    29461 cagcaatgtt ggaaacgttt ttaagaagaa tacctgtaac tataaagatt ccgtctttag
    29521 aggaaagagg ctttcaagag agaatgaagc ttatatgtta tttttttaaa gaagagagtg
    29581 ttaggattgg tgttaagtta aaggtttcaa aagaagttat taaggcattt attttatata
    29641 agtgtaaggg aaatataggc caattaaaaa gcgatataca attaatttgt gcaagagctt
    29701 ttttagatta tatgacctat aaaagggaat gtgtatatgt aaggctctct cttctaccaa
    29761 ataatattag agaatcatta tatggaaata atagaaaaga ggagtttgta caaaatttca
    29821 gttttattgg aaaagatgat attatttttt taccagaaga aaaggatttt gaaagtgaaa
    29881 atttattaat agaaaataaa aaatatgatt tagattttta tggaatgata aaagaaactt
    29941 ggaataaact tcaacaagaa ggtattaaag agtcacaaat tagaagtgtt atagatggag
    30001 acatacagaa gtattcctat aatttaatga atacttttat atataatagt agtaatcaaa
    30061 cagcttataa caacattgtt aatgattcaa ttgcaactac tgtaaaatat attttacaaa
    30121 agcatgataa atggagcaaa agagaaggat tagataaatt aataaaagct atagctttac
    30181 atattcagaa tttaattgaa agaataaaga ttggaaatat agtaaagcat cctaataaag
    30241 atgaaattat gaaggaaaga gcatatgaat ataacatagc aaaagaaata ttatctaaca
    30301 tgtcattaat gtatggagta gagtttccag aagatgaagc aattttttta gcaacatttc
    30361 tttatttatc gtatgtggga ttaaatgagg agagtatagc aatattagtc ataatgcatg
    30421 gtgaatctac agcatctagc atggtgaatg tagccaatac tttactggat tgtaaccatg
    30481 cagtggcaat taatatggga ttagaagata gagttcagga tattttagta caagctgtag
    30541 aaatagccaa taaaattgat aaaggaaaag gaattttaat tttaactgat atggggtcta
    30601 ttttaacatt tgctaaagtt attcgtgatg ctacaggaaa agaagtaaaa gcaatagata
    30661 tggtaagtac acctattgta atagaagcaa ctagaaaggc tctaactcca gaaatgacat
    30721 tagagaaatt atattttaat attatagaat ctataaagaa acattatgga gatatggaag
    30781 ttacatatgc tgaaaaagat aatggtggta gatattttga tagattacta attgataata
    30841 ttagtaaaac tttgactttt ttaaatgggg aaaaggctta ttttatatta aaagaagtaa
    30901 ttaatagaat tagtgaacac tattctttag taattcaaga tgaactttta gtaaagttta
    30961 tatttcattg ttcatgtatg attgaaagag taataataaa agagtcttta gtttataaat
    31021 catatgaaga aagaattagt agaaataaag aattatattt agttataaaa gaaagtttta
    31081 agctagtaga agagacattc gatataatta tttcagatat ggaatatgca aatatattag
    31141 atatatttga atcacaatat gatacagata aattcaaaag ttgatacaaa atttaaaaat
    31201 agccaaaagg ctatcttttt tttacttttt ttgatacaga tttgatacag gttgaaaaaa
    31261 tcttttgata catatcgaaa taataagttt ttaaaaaaaa ttgagtaaaa agttttgatt
    31321 aaatcctgta aaatagccat ttgttattga tacacataag ttggcacggc aattgcttat
    31381 ataaaaagtg acaagaaaag tgaggtgaaa ttggtgatag gaataatagt tatgactcat
    31441 gggagtttta gtgaagaaat tataaaaagc tgtgaactta ttgcaggacc agcagaaaga
    31501 acagcagcaa ttaaactaaa taggaatgat aatattgaag acttaaataa aaattttgta
    31561 gaaaagttaa atgaattaga tgaaggtgac ggagttttag tattagctga cctattagga
    31621 ggttcgccaa gtaatgttgc aagtttaaac cttaaaaaag gtggcaagtt tcatgcactt
    31681 actggagtaa atcttccaat gctacttgaa gctttaatta atagagaggg aaaaagttta
    31741 gaagagttag cagaagcatg tattgaagca ggacaaactg gtataaacaa tattaataaa
    31801 gttcttagct caatgtaata gaaaaaggag tttgattaaa aatgtcttta gtaaaatatt
    31861 gcagatgtga tgatagatta atacatggtc aagttatata taaatgggtt aagcatctag
    31921 gtgtaaaaaa gatagtagta gtagatgatg aaacaaccaa tgatgttatt gctaaaggtt
    31981 tgataaaaat ggcagcacca aagaatattg atttatcaat attaactgta agtgaatcaa
    32041 ggagatattt ttataataat caagctgatg ataacgtttt tgtattaatt aaaaatttag
    32101 atactgcaaa tagaatgata gaagaaggga ttaatatcaa aaaacttatt gttggaagaa
    32161 tacctacagg aataggaaaa aagaaaatat ctcaaaatgt atacattaat aaaaaagagt
    32221 ttcttttaat agatgaattt attaaaaaga atattaatgt atcaattcaa atggtacctg
    32281 atgaagaaga agtagaatta aaccaaaata ttaatcgcta taaaggagag tttatttagc
    32341 aatgaataaa tataaggtaa tcatactgtg ccatggaact tggggaaaag tcttagtaga
    32401 agaaacaaat aaaaactttg gattgtcaaa tcaatatgaa gttttatcac taagttctga
    32461 aaaagatgtt tctgtattta tgaaagaggt tgaagaagta gtagataaag aagatgtgaa
    32521 aattataata tcagatttat atggaggatc tacatcaagt gttgcaatag ctgttgctat
    32581 aagaagagga atagatgcaa taaatggact tagcttacag acaattttaa tcgtagatga
    32641 agaattaagc aaagaagata aattacatac attaccagaa agaatcgtca aaaaaaataa
    32701 taacctttgt gtggatttaa ttaaagaatt taaaaaaata taaattatga ggtgaatata
    32761 atggcaaaaa taacattagt aagagtagac cacagattaa ttcatgggca agttataaca
    32821 aaatgggtaa aaatcgctca agctcaaaaa ataataatag ttgatgactt cttaggtcaa
    32881 gatgaattta tggctgacat atataaaatg gcagcaccat caggagtaga agtagttatt
    32941 ttaacagcag aagatgcggg acaagcattc caaaacaaca ctttaggaga taaaaatata
    33001 tttattctat ttaaaaatgt tgatatggca aataaggctt ataaagctgg acttaaatat
    33061 gaaaagattc aattaggagg tattccaaat gaagcaggaa agaaaatggt gtttacagct
    33121 gtttcattag gaaatgagga tgtagaacaa cttaatgaac ttaatgaaaa tggggttgaa
    33181 atagtcctac aagttatacc agaggaatca tctatgacat atgaaaatgc gttaaagaaa
    33241 tttaaataaa aaatataaat taggaggaat tataaatgga atctgttttg atgttggcaa
    33301 ttgtaacagg attatggtat tggtttgcag caggtcttgc agggtatacc cttttctcaa
    33361 cactaaagtc tcctttattt ataggtttta gccttggatt actttggggt gatgtaacaa
    33421 ctggtatgat tgttggtgca agtatagaaa tggtttatct aggaatggtc gcagcagggg
    33481 gaaatattcc atcagataaa tgtctagctg ctcttattgc aataccagta gcacttcaaa
    33541 ctggtgttaa tgcagaagtt gcagtatcta ttgcggtacc tttaggtgtt ataggagtat
    33601 tagttaataa cttaagaaga actggtaatg ctgtattggt tcataaggca gataagtatg
    33661 ctgaagaagg taatacaaaa ggcatctgga gatgtgcaac actttattct ttaatatttg
    33721 ggtttgtttt aaggtttcca atagtttttg tatgtaactt ttttggagca gatttagttc
    33781 aatcattatt agatgttata ccacaatggt taatgaatgg attaacagta atgggcggta
    33841 tattacctgc attaggtttt gcaacaacaa tatttactat aggtaaaaat aaatttttac
    33901 caatgtttat tataggattt tttatggttc aatattttga aatatcaatt actgcagctg
    33961 caatatttgg tgtttgtata gctcttttaa ttacctttat gaaagaagac aaaagggtag
    34021 gtgaagtata atgcaagaaa taaaaaataa agaagttaaa aatggacaag gtccaactga
    34081 agttaataag acaagagtac ttacaaagaa agatgtaaca aaaacatatc taagatggtg
    34141 gtggacagca gaattatcca actcttttga aagaatgcaa gcattagcag tttgtgcaag
    34201 tttcactccg gctttagaaa agttatataa gagaaaagaa gatttagtag atgcgttaaa
    34261 gagacactta caatttttta atactcaagc tatatgggga ggattaattc acggtacagt
    34321 tttagctatg gaagaagaga aagctactga aggaaaaatc ccaggagaag ttatttcagg
    34381 tgttaagaat ggtcttatgg gacctttggc tggaattgga gatactttag attttggtac
    34441 atttcaaaca atattcttag ctttaggtgc ttcatttgga gcagaaggaa gtgttatagg
    34501 tgcatttttc ccaataatgt ttagtatatt actattttgc gaaggttatt acttatttca
    34561 tttaggatat tctttaggta gagattcaat taagaaaatt ctatcaggtg gtattgtcaa
    34621 taagataatt gatggtgctt caattctagg aatgtttatg atgggagcat tatctgcaac
    34681 tacagttaag ctttcaactc cgctatcatt tgatattggt ggtaaagcaa ttgttgttca
    34741 agatacatta aatatgattg ctccaggttt attaccactt ggtgttgtat tcttcgtata
    34801 ttggggaatg aaatacaaga agtggacaat aactaagtta ttagtaattt tagttgtatt
    34861 agcattggta gggtcattca ttggaatatt ctaattcaca gtgattaaaa tatagaattt
    34921 aattttagga ggagaaagaa atgtcagaaa aaatgtatac agaaaaggaa tatttagaag
    34981 gaattagagg agcaacagga gaaagagcag tatggttcta tcttcttatg aaagaagctg
    35041 aaaaattagg agctaatcca gatgatatat gtaaagaagc aatatatgga tttggtaaaa
    35101 tgagaggtca aaaatataac gtagcagata ctccaggaaa aatggcagaa atgttatata
    35161 acagtaaagg acaaaaagtg tttgaaatgg aattagtaga aaatacagat gagaatggcg
    35221 ttttaaaatt ccatcactgt ccattagatg ctgcttggaa agaatatgga ttaactaaag
    35281 aagaaagaaa agaaatttgt agattagctt gttatgggga ttatggaaga gttgactgcg
    35341 cacaaggtgt taaattagat tttgctcaaa aatgtgctca tgatgatgaa gtttgtgaat
    35401 tagtatttac aaaaaaataa tttttaatgc taaaagcctg ttctttaagg acaggcttta
    35461 aaaagataga aagtaggttg atttatggat ataagcagaa gtgtagataa tagtttaaat
    35521 agaaccatag aatttctaaa agaattgata aaaattgatt cacaacaagg tgaacctatt
    35581 tcacaatgtc cttttggtat aggacctaaa aaatctttag ataaaacttt agattactgt
    35641 gctagtttag gttttagtgt taaaaatatt gataattata ttggatatgc agaaattgga
    35701 gagggagagg aattaatagg gattccaatg catttagata tagttccacc aggagagggg
    35761 tggagtgtag acccattttc gggagcagta attgacaata ttatttatgg aagaggtgtc
    35821 atagataata agggtgctgt atctatgctt atacatgtat tgaaaaacat agaggatatg
    35881 tatcctacaa ttaataaaag aataagatta atttttggta caaatgagga gacaggtatg
    35941 aaatgtatca aatattactt agataaaggt gaagaaattc caagtatggg atttactcca
    36001 gatgctatgt atccagtggt taatggagaa aaaggaaggg ttcatataag gatagaaaaa
    36061 gaaattaaaa ttgacaaaag taaaccttac ataatagtaa ggggaggaac taaagaaaat
    36121 gttgttccat ctcattgtac tgcaaaaatt attaatggaa ttatttcaga gttaactact
    36181 aaaggagtag ctgtacatgc aagtaatcct gaaaagggag aaaatgctat atctaaaatg
    36241 gtaataaaga tagttgagga taatatggat tttcagcata gagaagatat agaattagtt
    36301 tcaaaatacc tttgtagtga ttattatgga gatgcattgg ggattaatca atatgatgag
    36361 gtattcaaaa acactacctt aaatttagga atattaaaag taaatgagga aaaaatcgta
    36421 tgtgagttag atataaggta tggtaaaaat atagttttaa ataacataat agatagattc
    36481 aaaaaggttt tttgtaatgg ttggaaaata gaggttattg ctcataaaga cttacattat
    36541 gtggatgaga gtaatttagt acttaaaaaa ttattagaag cctatgaaga agtaacagat
    36601 gaaaatggat atacaattgc tatgggtggt ggcacatatg caagttggtt taaagatatg
    36661 gtggcgtttg gacctaagtt cttagcgtat aaaactggtg gtcatggagt agatgaaaga
    36721 gtaccaataa atcatatacg aaagaatatg gagatatata ctttggcttt aataaaacta
    36781 ttagaattgt aaaagaagaa gagactttat gtgcaaattt acagatatct taaaaagaga
    36841 gataaaacga gttcaacctt atttaaatgt tcaacaccat caaatggggc tgtctcaaaa
    36901 tagagataag cctcttactt attgttgaaa tgatgaaaat atattttact tcatacaaaa
    36961 aagagtgcct tatgaagtaa aaagttaatt ttgagacacc ctcatttgat gatgttgaag
    37021 agaaaataag tatgaaatta ttaaaaaaga tgattttaaa aaagttaagg ttgaaaagaa
    37081 aagttaatta aatggaatta gataaatgga gaaggatgta tactatctaa taaaataaca
    37141 aaagagtgtt ataaagttgg ttatatgatt agagaaaagt ctattgatac ttatccagat
    37201 agtggatggg gatttatgac ttgggatgaa aatggagaat aataaaaatt atagtttata
    37261 cagtttaata agatatatgt acaagcagtt aatctaaatg attgactgct tttcttttat
    37321 agatttttat ataatagaca aataccaata catgatagtt gcctgttata attagaggaa
    37381 taaatacgaa taataacttt ataaaagaat taaatggaaa atcgaagagt tatattcagt
    37441 attttaaaaa gaatatgaaa tgagaaatga aaataaggtg tttatattaa aagaggtttt
    37501 acacaaaata tttaaaaacg tgataaataa aaactgtatt tagatagatt aactattagt
    37561 tttatataat atggatttta tttacaaagt ttacatattt tacaaaagag attaaagata
    37621 aatttaaata tatattttat atacttgttt atttgtttct ctgtggttga ttttttcgtc
    37681 aaaagtcaac ttcaaatcgc ttgttagctt tacatgttta aggatataat tatacataag
    37741 gagggattga catgaatgaa ttaaacatcg caaaaacgct gattttaaag cgtaaagaaa
    37801 aaggaattac acaagatgaa cttgcaaatt atataggcgt ttctaaagct tctgtatcta
    37861 aatgggaaac tggacaaagc tatcctgaca taaccttttt accgcaactc gcgacatatt
    37921 tcaacattac cgttgatgag ttaatatgtt atgaacctca aatgatgaag gaagatatta
    37981 ataaactgta taataaactg tgcaaagact ttacggttaa accatttgat gaagtcatgg
    38041 atgaaataag agaaatcata aaaaaatatt attcatgttt tcctttgatt tttcggatgg
    38101 gtcttttaat agttaatcac tatgatattg tagatgaaaa aaagcgtgaa ctattaatta
    38161 gtgaagcatt agaaatattt attcgtatac aggaaacttg taatgacata gatatttgcc
    38221 gtcaagcaaa aagtatggag gcaacttgtt atatattgtt aaatcaaccc atacaagtga
    38281 ttgatttatt gcaaaacagc aattttccca tgataaatga atctatactt cttgcacagg
    38341 ggcaaatgat gaatggccaa atggatgaag caagagaaac atttcaactt ggtgcatatc
    38401 agaaccttat atcattagtc caaaacctag tgggtatttt gcaaaatgca gataaattac
    38461 aaatgaagga aatagaaaga cgtattctag caatttcaga tatatttgag cttgatacat
    38521 tatctcctgc tattatgctt tcttcatatt taacacaggc acaaattaat ttaatacatg
    38581 gtgataatga aggagctatt aaatcattac gaaaatatgt agatttagca acacgtgata
    38641 tatacccaat tataatacat ggggatgact tttttaataa acttgacaga tggatttctg
    38701 aaattggaac aggtattaca cgtgatgata caatcgtaaa agcaggtatt gttgctgcta
    38761 ttaaaaataa tccaatgttt tctgtattaa gtgaaaacaa agaatacaaa tttcttatag
    38821 aaaaattatc tttattggag gaataagatt atggaaaatt tgatggaata cttaccactt
    38881 ttggttcctg ttattatttt agatttaata cttataatta ctgcacttgt tcatgtgttg
    38941 agacatccaa attataaaat tggaaataag gcaatatgga ttattgtagt actgtttatt
    39001 agtttaatag gaccaatttt gtattttaca ataggaagag gtgaagaatg atgaatattg
    39061 tctcaataaa tggattatca aaaggttttg gtaatcgaaa gataatagat aatttaaatt
    39121 ttactgtacc agaagggtca gtctttgggt ttgtaggtaa gaatggtgca ggtaagacta
    39181 caacaatgaa aatggtactt gggttactta aaccagatag tggaactata gatgtttgtg
    39241 gtgagaaagt gacctatggg aaaacaagtt ctaatcgtca tgtaggttat ctgcctgatg
    39301 ttccagagtt ttacaattat atgagaccac ttgaatatct ttctctttgt ggtgaaatta
    39361 cgggactttc aaaaaaagaa atacagataa ggagtgaaga gttgttatca cttgtgggtc
    39421 ttagaaatga aaagagacga ataggtgggt tttctcgtgg aatgaagcaa cgacttggta
    39481 tagctcaagc tttgctttca cgccccaaat tacttatttg tgatgaacca acaagtgcac
    39541 ttgaccctgt tggtcgaaaa gaaatattag atattatgct taaaatcaaa gactcaacaa
    39601 ctgtaatatt ttcaacacat atactatcag atgttgaaag aatctgtgac catgttgcta
    39661 tattaaacaa aggaagcatt gcccttagtg gtacactttc agaaataaaa agtatgcacg
    39721 gaaaggatag acttttactg gagtttgcaa gtaatgatga aattcaaaag tttaagtcta
    39781 gtgatggtat taaatcctta ctaaaagatt cagaagaaac taatatggaa attgttttgc
    39841 atggtaaaga tataaaagca atacaaaaaa cagttatatc tacactcgca gaaatgaatc
    39901 tttgtccagt aaagatggaa ctaatagaat tatcacttga aaatttattt ttggaggtag
    39961 taaagtgaga gaatacatag catttacaaa aaaagaattt aaagagaatt taagaaatta
    40021 taaattattt agtttaatta ttttatttct aatatttggt ataagtagtc ctatttctgc
    40081 aaaatttatg ccagacttga ttgctcattt tgcaccgact ttaaaagtta ctgctgcacc
    40141 aactgcttta gattcttgga cacaattttt tagcaatatc tctggtcttg gtatgagttt
    40201 aactctaatt attttttgta atattttatc aaatgaatat tctaaaggga cacttgttat
    40261 tatgctgact aaaggtttat cacgttcatc tatagtattg tctaaattct ctgttacggt
    40321 gattattatg acaattggtt tttggttgag ttttttattt gcttatggat atacaatgta
    40381 tttttggcca actgcaaatc ttaatcatat tatattttca gcatttaatt tatggcttat
    40441 aggaattatg tatattagtg tattaattct tgggtgtgta ctatttagac cagcatttgc
    40501 cagtgtttta cttgtattgg tggcaactct tttattgagt ttaatttcta ttcctaaaca
    40561 gattgcacct tatactccta atttcttgat gtcaaaaaat atagacttga tttcagggaa
    40621 agtggcaatg ccagaattta ttattccaat tattataaca gttcttattt ctgtagtatg
    40681 tttactagca gctgttattt tatttaataa aaaaccagtt taataatacc tttatcacaa
    40741 ggtaaagtaa tcttatgttg ttaaaaacta cacctaaaaa tcaaaaagac ttggacttaa
    40801 gtttcaagtc tttttgattt ttaatatata gaattaagtg tagaaaataa tacaaataat
    40861 attatttgta tcaaataaat ataagaatat ttaattatgt taaaatatac taaaaattac
    40921 tttaaaagtg ataaaattag tataagtaaa tattttaaag gggtgtagat atggaaaagt
    40981 caaaagtata tttttgtgat ttatactcaa attcgcaaaa taaaaacgta ccaaacaatg
    41041 taagaagatt atttgatgaa gcaggattca aagatttaat agaaaaaaat gaccaagtcg
    41101 caattaaact tcactttgga gaaaagggaa atactacata tatgagtcct gtggcagtta
    41161 gacaagtggt tgataaggtt aaagactgcg aaggaaagcc atttttaaca gatacaaata
    41221 cactgtatac agggagtaga acaaattctg tagaccatct tacaactgct atagaaaatg
    41281 gatttgctta tgcagttgta aatgcaccag taataattgc agatggatta tatagtagaa
    41341 attatgagaa tgtaaaaata gacaaaaaac attttgaaag tgtaaaaata ggtggagaaa
    41401 tttataattc ttctgcaatg atagtaatga gtcattttaa aggtcatgaa gcagcaggtt
    41461 ttggaggagc attaaaaaac ttagctatgg gatgtgcaag tgcagcagga aaacaaatgc
    41521 aacattctga tgttactcca gtagtaaagg aaaagaagtg tgtaggttgt ggaaagtgtg
    41581 ttaattcttg tcctacaaaa gctataagta tagtggataa aaaagcagtt atagattctg
    41641 atgtatgtta tggatgtgga gaatgtccaa ctgtctgtcc aactagagca gtaactatac
    41701 aatgggaaag tgactcagat gtgtttgtcg aaaagatggc agaatacgct tatggagcag
    41761 tatcaaataa aaaagacaag gtagggtata taacttttgt tatgaatgta actccacttt
    41821 gtgattgtgt accttggtca ggtagaccaa ttgcccatga tataggaata ttagcatcaa
    41881 cagaccctgt agctattgaa caagcatgct atgatttgat ttgcaaggaa atgggtcatg
    41941 atgttttcaa acatgaacat cctcatgtaa atggaactag aataatagac tatgcatgtg
    42001 aaatgggtat gggaagtaaa gaatatgaat taataaaatt ataagaatag tagcacttaa
    42061 atgcatataa ttattgtaag agaaatctaa aagtatcaaa ggagtatgca tttatgaaaa
    42121 tttactatga gaaggaaaac agcaagaagg ctaatgtaaa caatattcca agtgtatttg
    42181 cgtttaattt tctaagaaac tttttgacta gcaaagagta tgaggaaact agaaaagaat
    42241 attttataaa taatctaaca aaaagtcagg ttaatcaagc catgaaggat ttaaaatggc
    42301 tatttagaga atatgaaggc ttagaagtta ttacaatgga aagcttggat ggagttaaga
    42361 caaggatagt tttataaacc agtaagaatt atattctaat aaaaaatact gctttaataa
    42421 taatatgtat aatctatata ctaatattgt tttgaagcag tatttttttg tgtatagtaa
    42481 acttgtgtct aaaataaagt tgaccaataa atgttatata tgcaatacta tatataataa
    42541 gtaaactaca taaaaatatt aagttgacaa ataggaggat atatggaaaa atacataata
    42601 aaattaaaaa ataaagtatt gagagaaaaa gaaataagtt atgaagaagc tcttaattta
    42661 ataagccttg atacaaataa taaaaacgat tttgatattc tattaaaatc tgcaaatgaa
    42721 ataagagaat attttatggg aagaaaagca gatttatgta cgataatgaa tgctaaatct
    42781 ggcaaatgta gtgaagactg taaattttgt gctcagtctt cttattataa aactggtgta
    42841 gaagaatatt cattattaga ttacaatgaa atcttaaata gagctaaaga gatggagagt
    42901 aaaggagtgc atagattctc attggttaca agtggtaaag gtatgtcagg aaaggagttt
    42961 aataatattt taaatatcta tgaaggattg agggaaaata ctaatttaaa attatgtgca
    43021 tctttaggta ttatagatta tgagcaagct aaaatgttga agtcagcagg tgtgactaca
    43081 tatcaccaca atgttgagac gtgtagagat aattttcata atatatgtac aacacacaca
    43141 tataaagata ggataaaaac tataaaagat gctaaaaaag ctgggctaga tgtatgtgta
    43201 ggcggtataa ttggaatgaa tgagagtgaa gaacagagac taaaaatggc ttttgaaata
    43261 cgagaattaa atgtaaaatc atttccaata aacattttaa atcctattaa gaatacacca
    43321 atggaaaatt atgatgtatt agaaccaatg gaaatactaa aaacaacagc agtatttagg
    43381 tttataattc ctaatgtgta cataaggtat gctggtggaa ggctatcatt aaagggatat
    43441 gacaaagttg gatttaatgg gggagtaaat tctgcaattg ttggtgatta tttgacaact
    43501 gttggaagtg ggattgaaaa tgataaaaaa atgattatag agcaaggatt tgaattttaa
    43561 taaatactgc ttaaattaat taatatactt ttagataatt ataaaagtat attaattttt
    43621 ttttgcagat taatttaaat attaaaattg aattataagt tgcaatattt tgaaaaaagg
    43681 gaaaaatatc aaaatttata taaaatggtt gcaaatgaaa aaaagatgtg atagtatcta
    43741 ggtaaccggt taagtaaaac ggtttcctaa attttggggg tgaacttctt gagtataact
    43801 ataaaagaaa ttggcgaatt ggctggtgtt tctaaaacaa ctgtgtcaaa agtcattaat
    43861 aataaagatg aaaatataag tcaggcaaca agagaaaaaa ttttgaaaat aatgaaggaa
    43921 aaaaattatg taccaaataa attagcacaa agtttagtaa ctaaaaaaac taatactata
    43981 gggcttctta taccagatat acgaaatcca ttctttacag atgtatcaag aggtgtagaa
    44041 gataaagcta atgaagaagg ctacaatata attttatgta atacagatga agatgctaaa
    44101 aaagaatatg aaggaataag aacactatca gagaggatga tagatggaat tatatttgca
    44161 gcatcttcaa atacaaactg gaaagaagca aattataaag atataaaaat accaactgtc
    44221 ttgattgata agaagataag tatgaacaaa gaaaccttaa aaggaattgt taaaataaat
    44281 aactttgaag gggcctatat agctacaaaa catttattag atattggaaa taagaaaata
    44341 atatacctaa gtggtccact tcaaaatgaa atagcagtag atagattaga aggatataaa
    44401 aaggctctaa tcgagtgcaa tttaagctac aatcctgctt atgtatttga aggaaaatat
    44461 aagattgaat ggggacaaga atttataaaa aatttagaaa agatagattt tgatgcaata
    44521 ttttgtgcaa atgatttgat tgcaataggt gtcataagag gtcttaaaga aagaggatta
    44581 agtataccaa atgatataag tgtcgtggga tttgatgata ttcaaacatc aagtctaata
    44641 agcccttctc ttaccacagt aaaacagccg tcttatgata taggatataa agccagtgaa
    44701 atattgataa attgtttaag aggagataaa aaagaatcgt ttgatgaatt gattttcaaa
    44761 ccagaattgg tcataagaga ttcaacaaaa gaaagtgaat aacagcacga gagacataaa
    44821 tagatatgta aaggagtaat gtaatgggaa acatagttgt cataggaagt gtaaacatgg
    44881 acatggtatg ttctgtagat aaaagaccag aaaaaggaga aacagtatta ggtaatagtt
    44941 tttttacatc acctggagga aaaggggcta atcaagctat ctcagcttct aaactaggag
    45001 caaatgtaaa aatgatatca tgcatagggg aagatggctt aggagaggag ttaataagaa
    45061 attttagaac gaataaagtt gattatagct tagtatccag aaataatcac aaaagttctg
    45121 gtgttgctgt tataacatta tgtgaaaatg ataatagtat tgttgttgta ccagggacta
    45181 atgagctagt agatatagaa ttaattaaaa agaatgaaga agagataaag aatgcagata
    45241 tagtattgct acaattagag attccattaa aaacaataaa ttatgtagtg aatttctgtt
    45301 ttgaaaatag gattagggtt ttattaaatc cagcaccagc agtaaaacta aatgaagata
    45361 taatagaaaa agtaacttac ttaacaccaa atgaacatga atataagata gtttttgaca
    45421 caaatgaagg gatagaagaa gtattaaaaa aatatccaaa taaacttgta ataacagaag
    45481 gaaaaaatgg agctagattt tatgatggtg aggaaatcaa gcatgtatct tgtataagtg
    45541 ttgatgttca agataccaca ggagcaggag atacatttaa tggagcattg gcagtggcta
    45601 taacagaagg gaaaaattta tatacagcag tagaatatgc agtagtagta tcaggtcttt
    45661 ctgtaactaa actaggtgca caatctggta tgccctataa agaagatgtt gaaaagtatt
    45721 taaataataa ataaaaatct aatttgttca aatttaaaac tattaaaaat ggaggttaat
    45781 tatgagagtg agaaaaattt tatctttagg aattgctgga atactagcaa taggaatgtt
    45841 aacagggtgt tcaatggaag gaccaagtaa atcagataat aaaggtggtt cagacaagaa
    45901 agatttaact attggagtat ctacaataac tcttcaacat caatttttta tagatattga
    45961 tgagggaata aaagaaaaag ctaaagaatt aggtgtgaaa gttatagtaa atgacccaga
    46021 ccaagatgta gctaagcaaa catcagcaat agaagatttt atacaacaaa atgtagatgg
    46081 aatgatagta cttggaactg ataattcggc tatagtacca gcagtagaag gagcatttga
    46141 aaaaatgcca gtagttacag tagatgctgt attaaatact gaaaatatta caagttatgt
    46201 aggaacagtt agttatgatg ctggtaaaaa attaggggaa tacactaaaa aatacataga
    46261 tgaaaagtta ggtggaaagt cagaaatagc aatagtgaca gatttaaaat ctcaaataca
    46321 aatgcagaga atagatggat ttaaagatgc attaaaaggt tcagcaaatg ttaagatatt
    46381 aaattcacaa ccaggatatg acagagaaga atctcttaat acagtagaaa acttaattca
    46441 atctaatcca gatgtagata taatatatgc aacagctgaa aatagtgtac taggagctaa
    46501 agctgcactt gaatctgcaa aaaataagga tgttaagatt gtaggatttg acttaactga
    46561 agaagcatca agtggaataa cagatggaac tattcttgct atgattcaac aacaacctaa
    46621 agagatggga cgtttagctg tagaagcagt agtaaaagct ataaaagggg aaaaggtaga
    46681 aaagaatata ccagtaccag ctttacttta tgataaggaa aatataaaag actttaagaa
    46741 ttagtaaaat aaaatgagat tatttgaaag aaatcctatt taaaaaggag tgtcattatg
    46801 agtaatataa ttctgaagtt aagtaatatt gctaaagaat ttcctggtgt cagagcatta
    46861 gataatgtta attttgagtt atttcatggc gaagttcatg cactactggg tgaaaatggt
    46921 gctggtaaat caacaatgat aaagatttta actggtgcac attcgaaaac ttctggaaaa
    46981 tttatttttg aaggcaaaga aatagagaat atatccccag atatctctaa aaagataggt
    47041 ataaatgcca tatatcaaga actaacagtt tttgacgaat taacagtagc acaaaatatt
    47101 tttatgggaa aagaaataaa tggcaaagtc ttaaccaatg acaaaaaaat gaatgaagag
    47161 gctaaaaaga ttttcgataa tatgggaata gatataaatc caaatagttt agtcaaagag
    47221 cttagtatag ctcaaaaaca aatggtagaa atagcaagag ttttatcatc agaaactaaa
    47281 gtattaataa tggatgaacc tacttcttca ataagtaaaa aagagactga aatacttttt
    47341 agacttataa atgatttaaa agagagtgga gttagtataa tatatatatc ccataggatg
    47401 gaagaactct ttgagatttg tgacagaata actataatgc gagatgggaa aacgatttcg
    47461 actttaaata ccaaagatgt atcgtctgag gaagaattag ttaatttaat gatagataga
    47521 aagctagacc aatttttccc aaaaagaaaa gtagaaataa aagaagaaat tatgcgagtg
    47581 gaaaatctaa ctaagaacaa tgtttttaat gatattagct ttaatataag aaaaggtgaa
    47641 atattgggaa taggtggact agttggaagt aagagaagtg aaatagtaga agctatattt
    47701 ggacttagaa catatgattc tggaaaaata tatttaaaca atgaagaagt aaagtttaaa
    47761 actccatcag atgcaataga aaatggacta ggattaataa cagaagatag aaaaggtact
    47821 ggattgtttt tacaaatgag tgtcaaagaa aatacaacta tggctgggtt aaaaaagata
    47881 tctaagttta aatcaattat agatagaaaa aaggaaaaag agattttaga aaaatacata
    47941 gaagctttaa agataaaaac accacatatg aatcaagtta tacaaagttt aagtggtgga
    48001 aaccaacaaa aagctataat agcaagatgg cttttattac aaccagatat attgattatg
    48061 gatgaaccaa ccagaggtat agatgtaaat gctaaggcag agatatacaa tctaatggga
    48121 gatttagtgg agagtggtgt aagtataata atgatatctt ctgaaatacc agaattaata
    48181 tcaatgagtg acagaataat ggttatgaga gaaggtcata taagtggatt tttagaaggt
    48241 gaagaaatgg ttgaaaataa tgtattaaaa ttggctttcg gaggtaaaat taatgagttc
    48301 aataactaag cataaagaag aaaaatcttc tccattattg acaaaattaa caagtgtatt
    48361 tagagaccaa gggagtgcag ctgtaggact tgtaataatt tttataataa tgagtgtagc
    48421 ttcatctaac tttttaactt tagataattt aattaatgtt ggaagacaaa tatcaataaa
    48481 tgcaatactt gcagtaggta tgacatttgt aataattaca ggaggaatag atttaagtgt
    48541 aggagcagta attgctttag taggtacatt ttgggctaca acagtagtga attataatgc
    48601 acctatatgg gtaggtatga tacttgcatt agcaataggg acaatattag gagtcataaa
    48661 aggtgcaatt atttcaacac aaaaactacc tccattcatt gtaactttag ccatgttaac
    48721 gataataagt ggggcaagct ttgtatttac aggaggaaga cctatatcag taaatacaga
    48781 tgcatttaag atgctcggta gaggatatat tggaccaata ccaataccag ttataattat
    48841 gattatagtt gtaatagcag gacattttct attaaaaaga actaattttg gacgtcatgt
    48901 acatgcagta ggtggaaatg aagaggccgc tagattatgt ggagtaaaag taaataaagt
    48961 tatagtaaaa gtatacgcac tagctggact actaacagca ttagctggga taatactatc
    49021 atcacgtttg gcatcaggtt caccaactgt tggagatgga gcagaattgg atgctatagc
    49081 agccgttgtg cttggtggta ctaatatgat gggtggttct ggttctatag taggtacatg
    49141 tataggtgtc ggaataatag gaatattagg aaatggatta aacttattaa gtgtatcttc
    49201 atataatcaa atgattataa aaggtttggt aatgttattt gctatatgga taaataatat
    49261 aaaattaaaa aaatcagcta aatctaaata gtaattaaat tttagcttat aaactataaa
    49321 aattattaag tttttgtata tatttttata ctttaatttg catacatctt tatacattga
    49381 atgagaatat gctaagtgaa aaagtttact atggcttaaa tagatgagat tagacttgaa
    49441 attttaaatg taaaaataaa tcttaggagg aaatatgaaa aaaattctct tagatacatt
    49501 aaatagtaaa aaacaagaat acatagatta tttaaaagaa cttgtaagta taaagactga
    49561 agatgttggt catggaatac taggtggatt tgaaaaagaa ggacaggaat atattgaaaa
    49621 acttgcaaat tacataggtt ttagtgtgga tagacaagaa atgagcgaag aattaattaa
    49681 aaaagcaaaa aatatatata aagaaggaaa tctaggtcac aattatcaag atagatataa
    49741 tttaatttgt aagtatagtg atgatttacc tggtaagaca attgtattta atggtcatgt
    49801 agacactatg ccaccgggtg atataagtaa gtggaaatat aatccatatc gtgctacaga
    49861 agataatgga aaactttatg gtcttggaac tgctgatatg aaatctggac ttatagcatc
    49921 aatattagct gtaaagctta taaaagatag tggtctaaat gtacctggta atgtaaaaat
    49981 aatgtcagta gtagatgaag aaggtggcgg aaatggtacc ataaatgcag ttatgaatgg
    50041 tattgatgga gattgttgca taatttgtga accatcagaa caaaacttaa tagttgcaca
    50101 tatgggattt gtattctttg aagttgaggt taaaggtgtg tctcttcact gtggtagtaa
    50161 gtgggagggt gtaaatgcaa ttgagaaggc tatgttgttg cttcaagata taaaagagtt
    50221 ggaacataac tggcttatga tatataaaca tccactatta ccttcaccta caataaattt
    50281 aggtgtaata aatggtggaa cagcaggttc tactgtgcca gataagtgtg tatttaattt
    50341 atgtgtacac ttcttgccaa atataatgag ttatgaacaa gttgtaaatg atgtaacaaa
    50401 tgtcataatg acaagggcta atggagattt atggcttaaa gataataaac caaatataaa
    50461 catatatcaa tcagggttag gttttgagat ggacaaggat tctgattttg tagtgaatgc
    50521 acataaaata ctagaagaaa ctctaggaaa aaaattagag ataaaaggtt caacagctgg
    50581 caatgatgcg agagtaatga aaaatttagc tgaaatacca acacttatat tagggccagg
    50641 ttcaatagaa caatgtcatt caattgatga atatgttgaa attaaggagt atttagatag
    50701 tatacttatg tatgcaagtc taatattaaa cttataaaag taggaggaat atataaatga
    50761 aaaaagtatt aatagtaggg gaatcttggg taaaaaatat aactcatata aaagggtttg
    50821 atacgtttgt tacaactcat tatgaagaag ctgtaaagtg gttgaaagaa gcaatagaat
    50881 ctggaggata tgagacagtt catatgcctg cacatgtagc agcagatagt tttccgtata
    50941 aattagaaga gttaaatgag tatgactgta taatattatc tgatataggt tctaatacgt
    51001 tcttattatc taatagtact tttatagatt gcaatagtaa tccagataga cttgaactaa
    51061 tcaaagagta tgtaaataat ggtggggcgt taatcatggt aggtggatat atgtcattta
    51121 caggaataga tgcaaaagct agatttggag aaactgctat caaagatgta ttaccaataa
    51181 caatgataga taaagatgat agagttgaaa aaccagcagg aataattcca gaagttattg
    51241 actctgaaca tccagtatta aaaggtatac caacagaatg gccaaagttt ttaggatata
    51301 acaaaacagt agctagagat aattgtcctg ttttagcaac tattggagga gacccatttg
    51361 tagctgttgg cgagtttgga aaaggaaaat ctgctatctt tagttctgac tgtgcacctc
    51421 actggggtcc aaaagaattt actgattgga aatactataa taaattatgg gttaatatgc
    51481 ttgattggtt aacatgctaa aaattttata aaacttgaaa acctcttact tgataaaatg
    51541 aaacttacaa gtcaagaggt tttattgtat aaaatggttg tatggtagaa aatattttta
    51601 aatcagttaa aattagtcat tttggctgat aatcgataag ttattagaaa cagggaggtt
    51661 tatataatta atatggataa tttaaataaa aagtttttga tggaatatat agatgagata
    51721 cagggagaat taagagatat atcagataag atatggaaaa atccagaact tcagtataaa
    51781 gaatactatg cttcaaattt acaaaaagaa tgtttaaaaa aacatggttt tagtatagag
    51841 ggattagaag gtatggatac agcatttgtg gctagttatg gagaaggtag accagttata
    51901 gcagttttgg gagaatatga tgctttagat ggtttgtctc aaaaagtatc cactactaaa
    51961 gaacctattg tagatggtgg tgcaggtcat ggatgtggac ataacttact tggtacaggt
    52021 tcaataggag ctgtaattgc agtaaaggaa ttaatcaaac taaaaaagct gaaagggact
    52081 ataaaatact atggatgtcc agcagaagaa gatttatctg gaaaagtatt gatgataaaa
    52141 aaaggttttt ttgatggaat tgattgtgct tttagttggc atccatttga tataaatact
    52201 ccaataagaa taccaacact tgctaattac tctgtgaaat ttagatataa tgggattagt
    52261 gcccatgcag cacaagctcc ttataacgga agaagtgcac ttgatgcagt agaacttatg
    52321 aatataggtt gtaattattt gagagaacat atatttgata attgtagagt acactatgta
    52381 actactaatg gtggaaaaat gccaaatata gttcctgatt ttgcagaagt gtggtattac
    52441 ataagaggag ttaaaatgga gcatgtaaga gatgtatttg gtaggattgt tgatatagca
    52501 aaaggagcag cattaatgac tggtactatt atggaatatg acattataag tggagtatat
    52561 gactatattc caaatacaat attaacagat attctatcac aaaatatgaa attagtaggt
    52621 gtgcaaaact ataatgaaga tgactactat tttgcagata aattagctga gacagtgtct
    52681 atagataaaa gaagtagtgt ttctagtgta ttaagtggaa ataaggatat tactaagatg
    52741 aatctgcatg atgaagttac ggatgataca tttactcata ataactgtat atcattatca
    52801 ttagatattg gagatgttag ctacataatt cctacagcac agtgttcttg ttcagtttgg
    52861 cctattggta tttctgccca tacatggcaa tcttgtgcca gtgcaggttc tgatatggga
    52921 tttaaggcaa tgttattggc atctaaatct atatcatgtt caataaatga tgtaatgctt
    52981 gatgaattgg ttataaacaa agcaaaaaaa gaattgaagg atactgtagg tagttttaaa
    53041 tatataccaa ttatttaaaa cttaaattga tataagcata actaacttgt atgagttaat
    53101 ataagtatat aatttaatat tatttttaac ccaaccttct aattatttta aatttaagat
    53161 acaatgatta gaaggaattt tttaagtttc ttagaatact gtaaattata taatcttgat
    53221 aaaaagtgtt atactatata taatctaaat aaaaaatagt acaaaaagga gtatttatgt
    53281 ttggatatgt taaaataaac aaaatggatt taacatttag agaatatgat tattacaaag
    53341 cttactattg tggactatgt aaatacttaa aaagaaatca tggagaaatc tctaggtttt
    53401 ctttgaacta tgatataaca tttttaattg ttttattaac agcagtatat aacccagagt
    53461 ctataagtac agaggaagtg tgtatagtaa atccatttaa aaagaaaaag gtaattacaa
    53521 atgatattac agaatatgca gcaagtatga atattctttt aacgtattat aaattggaag
    53581 ataatttaat ggatgataaa cgcataaaag ataaattggc atattatata tataaaaaca
    53641 aattaaaatt agcatatgaa aagtatcctg aaaaagcaga gtatataaaa caacaactaa
    53701 atgaattaaa taagttagaa aaagataaaa atataaatat agatgaagta tctagtatat
    53761 ttggaaatat aatgggtgaa gtatttgtct ataaaaaaga tgaaaatgag agaaacttaa
    53821 gaatgatagg ttttaatata ggtaagtata tatatctact agatgcctat gaagatttag
    53881 atgaagattt taagaaagga agatataatc catttataga gtatattgat aaaaatgatg
    53941 agttaaaaga aaaagtaaaa aaaataatag taacatcatt aggattttta gctaggggaa
    54001 ttgataatct aaatctaaat aaaaatgttg gaataataga aaatataatt tattctggtg
    54061 tttatttaag atatattaat atattagaaa gtagaggtgg aaagaatgta cagtaatact
    54121 tataataatg aaaattttat gagagcaaga tcattcatag aaaatagtga atttaaaaaa
    54181 gcatatgatt ttttaaaaac tttgactgac aaatgtgcag agtggtatta tttaactggt
    54241 ttttctgcaa tgaacatagg atattatgaa gaaggtgaag acttcttaaa aagagcaaaa
    54301 tttatggagc ctgaaaatag tgaatatagt gatgcactta gaagttatac acaatataga
    54361 aatgattata gcaatagggc tgacaattat aatagaagaa gacgtaatga tttagatgga
    54421 tgttgttgct gttgctgtga tgattgctgt tgttgtctag gtgacgattg ttgtgaaaat
    54481 tgtgctaaac tttggtgttt agattcatgt tgtgaatgtt ttggtggaga tttaataact
    54541 tgttgttagg atgataaata tgagtataaa agttgcttat ggaggagttt tattagcatt
    54601 aaatgtgata ttgttgactc ttacaaacat tattcctgta aatacattgt ttattatggg
    54661 gttagcatca cttttagtat caattgtaat aatggaatgg ggctttaaga gtggaattgc
    54721 tttttatata ggttctatag ttttaggatt tatagtgatg gcaagtaagt ctcaatggat
    54781 tgtatattca cttacctttg gaatatatgg tattgtaaaa tatcttatag aaaaagatag
    54841 gtctatatat attgagtatt ttatgaaatt agtatttgca aatataatga tacttatatt
    54901 gtatttttta ttaagaacaa tagtttatat tccaataaac atatttatta taggttcatt
    54961 tgagatagcc tttatagtat atgactatgt ttacagttct tttataggtt attacaataa
    55021 tagattaaga aagatgctat ttaaaaaata atagttgatt tttatattta attttaatga
    55081 aattgtagtt tagataagtg taaatgatat atatgaatat acattaaagc ataaagctaa
    55141 atttaatata ttcttagtgc aacatataaa agctttgatt tttagtcaaa gcttttatat
    55201 gttagatata aatattagat ataatataag tttttaaaat agatagtata aattaaatat
    55261 ccactatatt tacatctgta gatagagaat aaataatatc ttttctttct actattatct
    55321 caacattttt agaaatttcc tcatcaagtg atatttgaga taaaagttcc tttgttggat
    55381 ttattgctat tggatttcca actcttttta gcatatttat atcaccatta gtatctccat
    55441 aggcatacga gtttgataaa tctaaatcat actttacaac aaaatcgttt atagcattat
    55501 tcttacttat agaatcccac attggaataa ctgtgcctgt aaatatattg ttttcaaaga
    55561 cataatcact tcctagaaaa tcggttacat tatatttttt tgccattttt tcaacaagaa
    55621 atccagggct accagatata aatataacta tgtgtccttg atttagatgc cactcaatcc
    55681 ttgaacgtgt gtatttataa actctatctg cttttaattt aattacttgg tcacttgtaa
    55741 agttgataca ggttttatct aatcctttta gtgaatcaac ataaaggtca catatttcta
    55801 atagataatc atcataatta ccttgtcttt tatcccaatc cataaaagtg tctctagcat
    55861 gtttatacca atttttttgg tcaattatat cgtatttaat taatttttta aaatgttcaa
    55921 ccataagaga gtctctataa agagtaccat ctatatcaaa aaaagctgct atatttttca
    55981 taagaccacc tcaattaatc atttccttat attatattat aaattgtatc taagttgtta
    56041 aaatatatgt ataataattt atatattaaa aaacaagtat tttagagttt aaaggatgtg
    56101 actatatgaa ttttaatttt gcaaaggcta caaattctag acttatgggc agtttaggtc
    56161 ttataataaa ttggatagat gatgaaaaca atcacttttg tcaatatttt ttattagatg
    56221 cagagggatt aggattagcg gattatgtga gtttaaataa cccaacacaa gaagaagctt
    56281 atatggaaga agaaagattg atgggaggat ttggttcaga tagagttgaa ttaacgaagg
    56341 atgaatcttt atttttagta tcttattttg gtaataagaa tctttattat gataagctat
    56401 tgccaggaga taaatgtgag tatattgata ttataaaaaa ctataaaaca gatttaacta
    56461 tagaaaaatt atacaataaa atatgtaaaa gagttgacga ggaagttgaa tttataaact
    56521 atatgaccat gagatttata gcatgggata gagaaagttt aaagtacttt tctggaagtg
    56581 acgagattgc aaatatgcac attactaata taaatggaac attacttaaa aacgtggtaa
    56641 gtgataaagg tcaaggcaga tatataagtg aagctttata tgaagataat gatggatatt
    56701 atatatgtaa aattgctttt tgcataagta agtgttatga aactggtttt agaattaatt
    56761 ctctattagt gacagataaa gaacctatgt atgattttga ggtatttgat gagatttcta
    56821 aaagtgaatt tgtaagtgtt tacagtgtaa attcacctga agaatttgca ggagtatttt
    56881 atagagaaaa tccatttctg ttaaaaagta atatgaatga aggtatattc tttactagat
    56941 ttaattttaa taatgaccat gtaaaagaaa atgtatatat aataaataat gatatgaaag
    57001 ctatatatta cttaatggga aataagtttt ttattggtac ttataacgaa aatgattgca
    57061 attatatcaa tgaaataagt caatctaatt atagcgacta tattaaattt gaagagaaat
    57121 ttttctttga gcaaaatgca ttatatgatt ttgcagaatc aggttcatta gattttgatg
    57181 attttttgga ataataaatt tagacattat ttaagcgaga ttaactgaat ttaatctcgc
    57241 ttaaatttat ttctataata ttgggggaat attatgagga atacatttat tatt
//
LOCUS       DAMJYC010000284        18345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.083, whole genome shotgun sequence.
ACCESSION   DAMJYC010000284 DAMJYC010000000
VERSION     DAMJYC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.083"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            complement(<1..723)
                     /gene="bclA1"
                     /locus_tag="PC472_004170"
     CDS             complement(<1..723)
                     /gene="bclA1"
                     /locus_tag="PC472_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2729386.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECPPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGEIGPTGVTGPTGNTGATGSI"
     gene            complement(1401..1925)
                     /locus_tag="PC472_004171"
     CDS             complement(1401..1925)
                     /locus_tag="PC472_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2729387.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(1990..2742)
                     /locus_tag="PC472_004172"
     CDS             complement(1990..2742)
                     /locus_tag="PC472_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2729388.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(2773..4071)
                     /locus_tag="PC472_004173"
     CDS             complement(2773..4071)
                     /locus_tag="PC472_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2729389.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYITKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(4088..6352)
                     /locus_tag="PC472_004174"
     CDS             complement(4088..6352)
                     /locus_tag="PC472_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2729390.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(6607..7425)
                     /locus_tag="PC472_004175"
     CDS             complement(6607..7425)
                     /locus_tag="PC472_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2729391.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(7418..8116)
                     /gene="cbiQ"
                     /locus_tag="PC472_004176"
     CDS             complement(7418..8116)
                     /gene="cbiQ"
                     /locus_tag="PC472_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2729392.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(8119..8397)
                     /locus_tag="PC472_004177"
     CDS             complement(8119..8397)
                     /locus_tag="PC472_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2729393.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(8387..9139)
                     /locus_tag="PC472_004178"
     CDS             complement(8387..9139)
                     /locus_tag="PC472_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2729394.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(9321..9513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9645..11417)
                     /locus_tag="PC472_004179"
     CDS             complement(9645..11417)
                     /locus_tag="PC472_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2729395.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(11431..12360)
                     /locus_tag="PC472_004180"
     CDS             complement(11431..12360)
                     /locus_tag="PC472_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2729396.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            12589..12777
                     /locus_tag="PC472_004181"
     CDS             12589..12777
                     /locus_tag="PC472_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2729397.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            12770..13471
                     /locus_tag="PC472_004182"
     CDS             12770..13471
                     /locus_tag="PC472_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2729398.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            13473..14396
                     /locus_tag="PC472_004183"
     CDS             13473..14396
                     /locus_tag="PC472_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2729399.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            14553..15476
                     /locus_tag="PC472_004184"
     CDS             14553..15476
                     /locus_tag="PC472_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729400.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            15469..16206
                     /locus_tag="PC472_004185"
     CDS             15469..16206
                     /locus_tag="PC472_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729401.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            16217..16990
                     /locus_tag="PC472_004186"
     CDS             16217..16990
                     /locus_tag="PC472_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729402.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(17531..18139)
                     /locus_tag="PC472_004187"
     CDS             complement(17531..18139)
                     /locus_tag="PC472_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2729403.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5933 a         3151 c         2278 g         6983 t
ORIGIN      
        1 tatacttcct gtcgctcctg tatttcctgt tggtcctgtt actcctgttg gccctatttc
       61 tcctgtcgct cctgtacttc cggttgggcc tgttactccc gttggtcctg tacttcctgt
      121 cgctcctctt ggtcccattg gacctgtaat tctaattgga ggacactcac atttaggata
      181 acaattatct ataattctat cttcaaatgt tacaagtata tacggtttgt tacaacctct
      241 actagatgta aattcaagaa tctgataagg gtaatttaaa gataaagcta acccataatt
      301 aggatattta ttcattgccc atgcttttat agttcccgta atatttaaac aaatatagtt
      361 ggatgtatca cttatcccaa ttggtaaata tcccctaata ttttccatag acactctatc
      421 attccatgtt atagttcgta aattataata ttcacttatt cttttaattt caatatctac
      481 tttttcttta aaaatatgat tcttatttga atcaatatga ataaataact ctgcttttaa
      541 tatacttttg tcttttaata tatctggtat tctcacttta aaaaaagtaa tcaacttttc
      601 ttttacaaaa ctatttgaac atttacttcc tattaagcaa taatctaaat tactaaagtt
      661 tttatctgga tattttttag atataaaagt atcatcattt aaataaagta taatatttct
      721 catttataat ccctccattt tgggtctata tcatactatg tttaaaatgc atgcttagtt
      781 acaatacaat taaaatttat agtattaaat ttttatataa taatgctgat ttctacaaaa
      841 aaagaacatg tcattagaaa tttctaatag catgttcttt ttcattatta tatattacac
      901 tttaaattat tccttgactg gcttagctgg tgtaaactaa accagtcttt ctaaaatatt
      961 aagcactttt ttcttattat gttttttagg atttattgtc tatattattg taaaccaaag
     1021 ttataaaatt gtaaatagaa tatattacca taacaatatt tttttataag taaaaattat
     1081 aaaaaacttt atttttgtta aatagaataa ataaaataag tttaaaaaat actgaaataa
     1141 gtgttttcca catattttat atatttattt aatacattat tatttaaaaa taaatatata
     1201 aacaatcata ataaaatttt tttaataaaa ctttattttt tagttaaaat catacaatat
     1261 atagatttcc acaatacaaa taaattaaaa agtgaaatgt atttgtctaa aaacataata
     1321 acatttcact ttatcctcat ttttaagtaa tatatatttt taatttttat ttataactaa
     1381 atctagtttt tatataaatt ttagtttttt tctacatctg agtaatctat tccaaaagtt
     1441 tctacagtaa cagatttcat aatttgtgga gtttgtggct tgtcagctga atctgttcta
     1501 actgatgcta tttcatcaac tgtatccata ccttctatca ctctaccaaa acctgcatac
     1561 tgaccatcta agtgtggtga gtttttatgc attatgaaaa attgagaacc agctgaatta
     1621 ggtgccatag ttctagccat tgatatgact cctctttcat gttttaaatt gttagtaaat
     1681 ccattaccac taaattcacc ttttattgag tgacctggac cacccattcc tgtaccattt
     1741 gggcatcctc cttgtatcat aaatcctggt attactctat gaaatattat tccattgtaa
     1801 tatccttcat ttactaatga tacaaagttt ttaactgtat ttggagctat atttggatat
     1861 aactcaattt tgatttcttt tccattttcc atttctatag ttactatagg atttttattt
     1921 tccataattt attaccttcc ttttcattag ttaaattaca taattacatt tattatatta
     1981 tactctaaat taaagtctat caagtatatt ttttcctatt tcatatcctt cttgataaat
     2041 agttttacaa aactcatcat caaagttaag cgtgtcttta ggagcaaatt gagtattagg
     2101 tctaactact attattttat cttcatcata tactttttta atatcttctg gcaactcata
     2161 attatatttt gtggatatta aaataatttt atccacatta tccccaatta atggttcaat
     2221 taatgcactt ctaattaaac ctccatctaa tttaaaacca tcataaattc cttcttgcac
     2281 atctttcaca aattgttctt taaatccaag agttgcgttt ggatttgatg gtaacaaccc
     2341 actgtatgaa atgctgataa tggcttcatt acgaggcatt tttgatacgt caacaatctt
     2401 ttctgatact actttattat caacttcaac ataattaaca taaaagttca tttcttctat
     2461 attactatca tctagttccc ttaggttgtc tatagcagga gaattatcca cagtattatt
     2521 tactattttt acatttccat tctggatatc ttttatattt gtccacattt tctccaagtt
     2581 atcaacattc cctgtgtaca aataatatcc atttattgct cctattgatg tccctgaaat
     2641 cgaataaaat ttatttattc ctccatcata caaggcttta actactcctg cttgataagc
     2701 cccttttgct cctccacctt ctaaacataa tcctaatttc atagtctcat ctccaaattg
     2761 tttatcttat atttatattt caaaaaactt actaaatgtt ttatatatac tatagtggtc
     2821 ctcaacattt cctaattctc ttatagaatg cattgctaaa attggacttc ccacatccac
     2881 agatggtata tctatatgag tagaagatat tggacctata gtacttcctc ctctttcatc
     2941 tgacctatta acaaattctt gatactcaac attagcttct ctacatatat ttttataaat
     3001 agctatagaa tatccatcac tagtatatgc ctgactagca tttatcttta taaccggacc
     3061 ttttcccatt attggtttat tagttgggtc atgcttttct ggaatgtttg gatgaatagc
     3121 atgagctaaa tctgatgata tcataaaaga tgaataaata gactcaaaga atccctctct
     3181 atccttaccc attgatatgc aaattctctc caatatattt aacaacatgt ttgaatctgc
     3241 accttgttta gtagagctac caacttcttc attgtcaaat acagccatta catttacacc
     3301 atgttgacct ttagtattaa caagagcatt caaactagca tgtaccattg caagattatc
     3361 ttgtctccca atagatataa attcatcatt agcccctatt aaacttcctt tttcaaattc
     3421 atataaaaat aaatcaaaat ctataatatc ttccatattt acatctaagt tagatgctat
     3481 ttgctttata agaaaattat ctttttctaa tgtatcattt aacatcccaa ctaaaggtaa
     3541 catatctttt tgcttattat atgagtgacc atcatttata cttctattta aatgtattgc
     3601 tagatttggt attatacaaa ttggcttttt aaagtctata agcctttcat ttggttttaa
     3661 tatactatca ccttttaaaa ctactctacc agctatccca agaggtctat ctaaccatgt
     3721 acttaatatt gctcctccat agcattctgt atttaacttc aaataagatt tttcagcttc
     3781 tatttctgca ttcggtttaa tactaaatgt aggagaatct gaatgagagc ctattattct
     3841 aaatccatct ctttctatac cttctgaatt tactacaaaa gctactatag ctgataaatt
     3901 cttagtaata taatactttc caccaactct tagattccat ctttgattca aaactaattc
     3961 ttcaaatcca tttttatcta agatttcctt agtactctcg actgcatgaa aagacgacgg
     4021 actatcatat atgaagtcta ttaagttttt agcaaagttt atactatcca ttcctcattt
     4081 acctccatta taaaatattt agtgttgtat attctttatt tattttttta attccctgtt
     4141 ggtcaaaagc tatagtaaca tctgtaccaa ttatagaaac cactgtccca accccaaatt
     4201 taggatgatg taccttagaa ccaagtttta tatcatctgg attagtttct ttattagaat
     4261 cttttattgt agcattaact ttatctgcaa ctttagattt gcttatagta ctcatatact
     4321 ttttagtata tttatctaac atattataac ttgctttaga atagcttagt tcttttttta
     4381 ctttgtacaa tctttctata cattcttctg gcagttcttc cataaatctt gaagctattg
     4441 caacatttgt tttaccatat aatgttctct tctctgtcaa tgacatatat agctcttctt
     4501 tagctctagt tataccaaca taacaaagtc ttctctcttc ttctatttgt gaatcactca
     4561 tagattttat agctcttgat ataggaaata acccttcttc catacctgtt aaaaacacaa
     4621 cagggaactc taaaccttta gatgtatgaa ttgtcataag cgaaacccta tcattgtctt
     4681 cttcatcatt tgattcagat gtaagtgcta tacttgttag gaatgtctct aagtctttat
     4741 tttcataagt atcatcatta ctactttcaa actccaaagc tatagaaata aattctctca
     4801 aattatcaat tctgtcttga gcttcttcac ctttgcctgt taaatctttt tcatttttat
     4861 ttcttatctc tacaagctcg tccatatatc ctgtagtatc caagactttt tctataagtt
     4921 tgctaacagg ataaacttct tttatagttc taagagtccc tataatatca acaaatccac
     4981 ttatacttgc ccttgcctta gtagatatat ctgaatttgt ttctatatca attaatactg
     5041 agtatatact ttcctgtttt aagttagctc tatcttcaat tttttctata gttctaagcc
     5101 ctatacctct tctaggaaca tttataatcc tttttataga tatatcatct tgtggatttt
     5161 gaataactct taaatacgcc aacaaatcct ttatttcttt tctctcatag aattttgtac
     5221 caccatatat attgtatgga atttgacttc tatttaaagc gtcctctaca ggtcttgcct
     5281 gtgcatttgc tctataaagt actgcaaaat ccttataagg tctattttct tttctagcta
     5341 tttgtgcaat tgaatcagca atgaaatcag cctcttctat ttcgctacca gttaattgta
     5401 tctttataag ttctccttct tttttttcac tccaaagctt ttttcttttt ctttctatat
     5461 tattagatat gactttatta gctgcatcta atatcacttg tgtagaccta taattttgtt
     5521 ctaactttac aacatgtaca ttgtcataat ctttctcaaa ttcaagtatg tttcttatat
     5581 ctgcacctct ccatccataa atactttggt catcatctcc aacaacacaa atattttgat
     5641 gttctcttgc taataatttt atcaattcat attgagcttt acttgtatct tggtactcat
     5701 ctaccattat gtatctaaat ctacttctgt aatatgctaa cacttcatca ttagccttga
     5761 aaagttctac agttttaagt attaaatcat caaagtcaag tgcactattt ctttttaaac
     5821 ggtcttgata tagcgcataa atatctgcaa tcttaaccat tctattatct gccatattga
     5881 tatctttaaa ctgctttgga gtataaagtt tgtcttttgc tcctgaaatt gcacttataa
     5941 ctgcttttgg ttcaaacact ttatcactta aatttaattc ttttagacag tctttaacca
     6001 atgtaacttg gtctgctgaa tcataaatta caaaacttct attgtaacca atcttattta
     6061 tatcttttct tagtatacga acacagcaag aatggaatgt acttatccac atatccttag
     6121 tttcaggacc tatattttgt tcaactctct ctctcatttc atttgcagct ttgtttgtaa
     6181 atgttatagc taatatattt ggtgcttgta cacctttatc ttctataaga tatgctattc
     6241 ttgttgttaa taccttagtt ttacctgaac cagcaccagc cagtataagt actggacctt
     6301 cagtcttttc tactgcttct ctttgagcag gatttaatgt atctaaattc atatttctcc
     6361 tcctgttaac agccataaat atattttaat ctaaaatatg aattacttca aattataatt
     6421 atgtattata ttaatagatt atttccaata tattacaatt aaataacatt tttccatata
     6481 atttatttca ttgtttaata aaaaaaataa ccttatgatt tggggaatca taaggcacct
     6541 ttaaggggtc taggttaagg gttattatat ataaatctat ttttttaaaa taggtttagg
     6601 gcttaattat ttatttgtat taagttctct ttccttccaa tacttataca aatcttcttc
     6661 tttcttaaat aagggtaaat tcatatttct atgtactttt actaaccatg gggattctag
     6721 gccagcttgt ataatattat tttcatttat aaacacattt tctgcttttc cttcatcaat
     6781 caaaatcccc ttatccaata catatatgta atcacatatt tcatacatta aattcatatc
     6841 atgactggat attactattt ttatattatt tttatttaat ccctttatta tatctacaat
     6901 agacctagta cttactgggt ctaaaccagc tgtaggttca tcaagtaata caatttcatt
     6961 ttccattgct ataactgatg ctattgccac tctttttttc tgtccatagc taagaaaatg
     7021 tactggcctg tctataaaat ctattccatt aacagctact agagctttgt ttattctctc
     7081 tttaattgtt ttttcatcca taccaatgtt tctcattgca aaggctatat catcatatac
     7141 ttttgaataa aaaatttgtt tttctgggtc ttggaagaca atacctacat tttttctaag
     7201 gttgtataaa cttctcttat catattttaa cttttcttct ttaaaaagaa tttctccaga
     7261 tgtaggcttt aatataccca ttaaattcat aaataatgtt gattttcctg aaccatttga
     7321 acctattata cctataacat ttccctttga aaaatccata ttaatgttta atagtgcatt
     7381 ggtatttttt tcatattgat acgttaaatt atttatttta aacatcatta tcccctacta
     7441 tgtgaaattt accatcatac aattttatgt ctaatgatat acacatatca tcataccttg
     7501 tcataagtct tttgtacagc atacttccaa gaatacctaa ggatttatat gaagttttta
     7561 aatttatata tccaaatctt agctcttgtg acttgtatat ttctgaaaat tcttctaaaa
     7621 atatgaatat aaacctatat attaacattg ataactcgac aaaagtgtca ggtaaatgaa
     7681 gttttttaaa aaagaatatt aattgattaa aaggtgttgt aagtatacaa aaatacacac
     7741 aagtcaaaca agacatcgct ctaaataaca catgaataga catgtcaata gacgcctttg
     7801 atattcctac attaaaacta aaaaattcaa aactatataa taaatctgct ttattaaaag
     7861 atatatttac aagagttatt cctatactaa gaaataaaaa gtacattggt attctaagta
     7921 attttaaata attttttaaa tctattccag ccacacaaac aaccaaaata ctcattaaaa
     7981 tcataattcc acttaaaata aatatatttt gtattaccat agaagcaatc agaaaaatta
     8041 ctcctatggc tacttttttg tttggattta ctttactcaa tctatttgta tatgcatatt
     8101 tatcaattat aagcatgatt aacttctctt tcctttttga agacctaata tgtatcctat
     8161 tacaccagcc cctaaagctg cttgtgcaga gaataataaa ctttctattt ctccacttgg
     8221 tggctcatat ggactgctaa accatggttt gtaatttgga tttatcttag taatttcact
     8281 ttcagcttga ccatctgctc ctccatattc agcacctgag ttgacaagta atgggaatat
     8341 aataagagct actactaata aaagtaataa tatgttagtt tttgttttag cactcattag
     8401 ctttaactcc tttatcatta ttttctagta tgcttttttc tccgttttct gtaattaaat
     8461 tatatacaat tacagtaagt ataccttctg ctattgctat tggtatttga gtcataaaga
     8521 aaattcctaa aaatttaatt gctgaagcca ttaccccacc tgatgggtct gggaaagcca
     8581 gtgctaattg tattgaagtt attgtatatg tggctaagtc tcctattgct gctgcaagaa
     8641 atactggaat agcattattt ccatcttttt tctttaaagc cttaaatatt aggaatgata
     8701 cgattggccc tattatcgcc attgaaaaag cattagctcc taaagtagtt attcctccat
     8761 gtgctaataa caatgcttgg aatattaaaa ctatagttcc aagaacaaac attacgcttg
     8821 gtccaaacat tattgctcca agacctacac cagtaggatg tgaacaactt cctgttactg
     8881 aaggtatttt tagtgctgat aatacaaaca caaatgctcc acataaagct aataatactt
     8941 tctttttagg gtcttgcttt actattttac ctattgattt taatccgaat aagaaaaatg
     9001 gtataaatat aactccccat gctatagacc attttactgg taagtaacct tccattatat
     9061 gcattgcatt tgatgtaact ggagttaaaa tgattaacat aagtgcagct ataaccccta
     9121 atttaatatt ctgtttcatc ttagtatccc tctctccctt tctaataatt aaaacataat
     9181 aatacatgtc tgatttattt aataaaataa aaaagcctaa ctcgatggtt aggcttctaa
     9241 ttttccacaa tctaaataca aacacataaa acaaacatat ctgtataatt attataagaa
     9301 actttctcca tccccgaaga aattacttta atattttagg caggtctcct gacttagctt
     9361 catcctactc ctcttccttc ccatgagata taaaaaatat catcatagtg gctaacaaaa
     9421 gtttcgtctg catcacagta gcgggggctg taaaggaatt taaccttttt ccctattaat
     9481 cttgcttaaa gaacctataa tattatatat ttaattttta aattcttctt aaattatttt
     9541 ctatataaat cattgtatac cttttctgtg aaaatttcta cattttttat aaattttatt
     9601 catttttata tttttatgta tataaattct tttttgtaag tttattaatt tctaattatc
     9661 ttctttaaat acattgaaac tacatcagaa aatctagtta tatctcttga atatataaaa
     9721 tcatttccat atattaatct ttcagcttca agttctgatt cttttcctgt aaatactcct
     9781 aatactaata tattttgctt tctagctttt ctcacttcat tggctgtatc tctcaaacca
     9841 actatacccc tataagcctt ttctcctctt aatgttctct cactactttt tcctatcttt
     9901 acatcatttg gtcttccatc gctgagaact atcaggattt tattttcttc atctctattt
     9961 attagaccac tacatataga ttttattgcc aaaccatctc tattattacc agcacaaaaa
    10021 tattcaaata tattttcact attttttata ctatcatcat aatctttaaa tctttttaat
    10081 attgtatagt ctaaaaaact agaaaatccc atcactctat ttggtatgcc aacttctgtt
    10141 agagcatttg caattatgta agcttgaatt gccacatttc cctgatttct actttgagag
    10201 ccacttgcat ccaataatat atcaataacg tatttccctt tttcattttc tatatcttta
    10261 taaaatattt tattattatt acttctacca actctccaag ctctatttgc taatatagta
    10321 ccacaatcag aatatactct acttttagca ttttcttcta ttaaaattct tgatagactt
    10381 tcttttaatc tctttatttg ctgcctatac attctagcat gttctctaaa ttttcctaaa
    10441 ttattttctt tttgtcttgt tacatatttt agttgaaata cattattaca ctcacttcta
    10501 agaactccat ctgtaaagtg tattctacaa ccttcatgaa catttctaca attttgtgtc
    10561 tcaatttttt taatttcacc tttactcaaa taagtttttc cataataatg ttctattttt
    10621 gaatatatct tatttgcagt gtcttcatca acatatataa ctcggtctgc tgaattgcta
    10681 catttagaat catcaaaatc ccctacacct tctacaagca tggatgaaga tattgaatct
    10741 atatcacttt caataatatt ttcttcttca tcactataaa gttcttcata catgaaatca
    10801 gcaaatgtat caaaatttac ttctacactt tctacattat tttcatcaat aagactattt
    10861 tcttctccat ttaatatgta ttcaaaataa gttaaatata taacatctaa tgcttttaaa
    10921 atctccataa tatcttgatt ggtatctata tttcttatat cattaactat tcttctagtc
    10981 atactatcta atgctgggtg cttgtccata ctataaagta caaatgtgta tttaatttta
    11041 tccataatat tatcattgtg tattttggta aaacgtgaaa caatatcttt ataagcattt
    11101 cttcttatgt cttctatccc tggcctatct ttaatgacct tttcttcaac tattgtattt
    11161 aatataactt gaattagacc cagtataata tctttgttgt atccttttct tattctattt
    11221 gtaagatatt ttttaactat atcccaatct acatactttc ttctagcacc tgcaattatt
    11281 gcaaaatata gacctatttc ttttgaagag taatcttttt ctgatatatc tatactttca
    11341 ttgtaatcac cagctattgt ccatgataag ttattagctc tattttcaaa ttcataatca
    11401 tcatatatcc atttcataat ctcacctata ttaaatatga cttattggaa acacatctat
    11461 actctcccat ttattaggta ttctagtttt aattacatct ccaaccattt ctttctcata
    11521 tacatcaaaa gtctttcctg taagtcccat attaattgca agagttggtt ttaatcctct
    11581 tcttatagtc ttaagagatg ccataagacc acgtaaatca atagctttac tagatatctc
    11641 tccattttga gacttcaatt gtaaatctaa aaatattcct gcaaagtgta ttagtttttc
    11701 ttcatcagca tctgaaaatt cattttttaa aatcatcatt aacttatctt cttctatagg
    11761 aggaatatct attaccataa atctagatac cagtgcttca ttaagttctt ttgttcctgc
    11821 atattcataa ttcatagtcc caataaatct tgttgctgga tgtagattga ctctctcata
    11881 cccaggtaca tcaattattc ttctatagtc taaagctgaa tgtaccacaa ctatagcatc
    11941 attttttgcc atattaattt catcaaacac cccaaaacct ccattaatag cacactcata
    12001 aactgagcct cttctaagtt taacttcatt gtctataaat gtatctgtac ctataagtgt
    12061 tgaactgtct gtatttatat gaaatgatgt attccactga ggtctattaa atatttcccc
    12121 tagattatca gcaagtaagt tttttcccgt tgcctttggt cccgataata atatattttc
    12181 ttcttctaaa atagctgcta tacacataga caagatatct ttaccataaa aataagtttt
    12241 agactttgtt actctgtatt ctaaatcttt ttctaatcca taataatttc taaaatacag
    12301 tacatcttca ataagttttt catctacccc ttgcaccctt agattatcta ttatatccat
    12361 atttttaccc cttttatata aatagtattt gtttcatttc ttatcaccat tttagcataa
    12421 aaggatataa gctaaaattg attttttaat ctcccccatt ttaagtaaaa ccatgcacca
    12481 ttttatcact acaaaattaa gagtactact tactgatatt tcttgatttt attcgtctat
    12541 acgtatataa tatatactga taaattatga ggaaaggatg attgaaatat ggaatatatg
    12601 tcttgcccag aagtagcaaa aaaatggggt atttctgaaa gaagagtaca aaaactatgt
    12661 gaagaaagtc gcataccagg agtttcaaaa ctcgggtata tgtggctgat accaaagaac
    12721 gcagaaaagc ctgttgatgg tagaacaaaa agaagaaagg aagtacaata tgaatagcgt
    12781 tttgattata gatgatgaca aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga
    12841 aaatttatct gctattactg cacatggtgg tgcagaaggt ctacatttag tcgataaaaa
    12901 tagacacaca ctctctcttg taattttgga cataatgatg ccagatatag acggttttca
    12961 agttctgaaa aaaattagag aaacaagtaa tattccagta attatgctta ctgctaaaaa
    13021 tgacgaggat gataaagttt ccggactacg tttaggtgct gacgattacc taacaaaacc
    13081 gttcagcata aacgaactta tggctcgtgt caattctctt attcgtcggt atactatgct
    13141 aaaccctgct tttataacag acacagattt cataataata aatggcatga ttattgacaa
    13201 gacaaaccga attgtttctg tcaataatat tcctgttcaa cttacaagca aagaatttga
    13261 tttacttgct tttctggctt ctaataaggg acgggtgttt accaaaaaac agatttatac
    13321 acaagtatgg gaagaagaat atgcttttga cgatagtaac atcatgtctt tcatcagtaa
    13381 attgagaaaa aagattgaac cagaccccaa ccatccattt tatattttga cagttcgtgg
    13441 tgtcggttat cgttttaata aggaggctta acatgaatac aagtgtctat ctgcttttga
    13501 tattggctct tagcctgatt attatagcgt accttgtttc tgtcctgcta cgtgtccgaa
    13561 tacaacttac acttataaaa gatgctttag aggatataaa aaatggaaac cttaatcgac
    13621 gtattttaac agatgaaaac gacattacaa aacaaatttg ctatgatatt aacgaaatag
    13681 caatcaatag ccaatttcaa cttattcagc aaaaacaatc agaacaagcc tacaaacagc
    13741 taatgacaag tctctcccat gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg
    13801 aagcagtaga aagtaaaatt gttgtaggag aggaaaaaga cgagtatatc catgttgcgt
    13861 ctaataaggc tcattactta aagaattttg tagaaaatct atttgaatgg gtaaaattgg
    13921 attctaagga acaagttttt cattttgata tttttgactt gaatgaatta tcccgtaata
    13981 ttatatctga ttggatacct gttttagaaa gcagtcattt tgaatatgaa tttgatatac
    14041 cagaaataga gtacttctta cgtatagacg caaatgcata tacccgtatc attaataacc
    14101 tattacaaaa tattattacc catagtagtg gcaacaaaat gacattgaga atttttgaaa
    14161 acaaagagca agctcaaatt ataataacag ataatgggaa aggtatatct tcagacaatc
    14221 tcccacatat ttttgaaagg ttgtaccaat gcgaccattc acgagcttca aagggaaatg
    14281 gtttaggctt ggcgattgca aaggaactta ttaacgctca taagggaaat attaccgcca
    14341 acagcactcc cggtatggga acagaattta caatattgct tcctaaagcc ctgtaaaagt
    14401 acgaggcttt cttactctaa aacaagataa aagcaagatt atggcaaggt tttggcaagg
    14461 ttcatgtttt ataattctaa ttgtagttga ttttgacgtt gtatcaacta tcttttgtag
    14521 aaacaaattt attaaaagtg aggaaattat atatgaatga ttttgtgatt gaaacaaagc
    14581 aacttacaaa aatttatggg aatcaaacag tagttgacaa agtcaatctc catgtaaaaa
    14641 aaggccgaat atatggtctg ttaggacgca acggagctgg gaaaaccaca attatgaaaa
    14701 tgattttagg attgacttca atcacgtatg gcgaggtaga tgtatttgga gagaatatta
    14761 agggacgtga aaaacaagta tatccccgta tcggtgcaat tatcgaaacg ccaggatttt
    14821 atcctaatct gacaggaacg gagaacttgg agatttttgc aaaactgcgt ggtacagccg
    14881 cccctaattc tgtaaaaaga gcattggaaa ttgtaggctt accttataaa aataaaaagc
    14941 tgtttagtaa atattcactt ggcatgaaac agcgacttgg tattgccaac gctatcctgc
    15001 atgacccaga actgttagtt ttagatgagc caaccaatgg tcttgaccct attggtattg
    15061 cggagatgag aaagtttatt agggacttga gcgttgagca tggaaagaca attcttattt
    15121 ccagtcatat tctttcagaa atctcattgt tggctgatga tattggaatt atcgaccacg
    15181 gtgttctttt ggaagaaagt agtatgagtg aactagaaaa gaaaaacagt aaatatatcc
    15241 tgctacaagt ttcagatatt ccgaagacat cacttatttt agagcgtcaa tttaacacga
    15301 aggattattc cgttcaagat gactacacgt tacaccttta taacactaat ctggatatgg
    15361 gtgaaatcaa taaagcactt gtaatgcaga atgtaactgt tattagttct caactttgta
    15421 atgatacttt agaggactac tttaaaaaaa tcacaggagg agagggcatt gcttaaactt
    15481 attcaaacag aatttcttaa actgcgtcgt aggaaactta tttggctgat gttgttgtct
    15541 gctctcatta tgccgttttt cgcactacta tacttcaatt attttggtaa aacaggtgtt
    15601 gagccaataa agttttataa atggtctgca tttggttata cactttggat tattttgcct
    15661 gttgttttgg gtatattttg caccatgctt atacacgaag aaaaccagta tgatatgctc
    15721 aaacagcttt ggattgtacc tattagtaaa atgggatact tttttagtaa atttttcgtt
    15781 gttttgattt actctatttg ttttatgctg attacagccg ttgcttctgt gttatttagt
    15841 gtactttctg gatatgtcgt atttagctgg gcaagcattt tatatttgtt gaaaaaatgt
    15901 ttagaaattg gtgtaatcac tgcttttgta atgttgccga ttttggcaat cgctacatct
    15961 caaaaaggat acattctccc aatttgtatc acattggtat atgccttttc cggttttgtt
    16021 ataatgacaa taaatatgta tttacaccca ttatccagta tgggagttat tatcatgaga
    16081 aataaagaca ttccaggtct tatattttca caggaaatta atgtcccatt agcatttctt
    16141 tgtatttgta tttggagtat tgtatcagta ttgtttgcaa atattgcact aggaagaaga
    16201 aagtgaggtg tagataatgt ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc
    16261 aaaaattgtt tggattacag tttttgcatc cattatgata gcagtcatag tttttgcagg
    16321 ggggcaggat gtatataatg acccagattt acattatggc ttaaagagcg tttatgacgg
    16381 ttcacggtat atagacaatg caggttggta tatggacgca gtacaacctt ggtcaacttt
    16441 ttttgttctt cctgctgtca ttgccctatt aggaagctat attatttgtc gtgaagaaga
    16501 ggaggacact attaaatccc tgcgtcttat tcccataaat gaaacaaatt taaccatagc
    16561 aaaaatgatt attactttta tattcagtat acttctttat ttgttactct ttactataac
    16621 atttttgact gaatccgttt tacatttttc cgatttatcg acaaaattgg ttttaagttg
    16681 catgaaagag tattttcttg atggcatagg tgtattcttt gcaatttctc ccattattgc
    16741 cttggtatcg cgcatgaaaa aaggatattg gcttgcatta gtattcacag aagtttattc
    16801 tattgctgga ctatttgcgg gtatgtcaaa tgtcttacaa accttttttc ctattgcggc
    16861 aatatttaat atttctggat actatataac tactggagaa aaaattatgg ggagtatcat
    16921 aagtttatta ctttgtgcct gcctttctgc atttatactt aaagggctaa aacacaatga
    16981 aaaaaattga aataggtata tcaaaatgaa aaagataaaa aaactgatta ttctaattgt
    17041 attcttgttt ctttttggca tgttacactg ttatactgaa tggaaattca acaataaccc
    17101 acctaaaggg gtaatagaaa gaataaatga gtggactgat aagattggac tggattagta
    17161 acttatgtta attatagtga atgtaatatt agcaagttat tgtttcgtat tttaatacta
    17221 taactttaaa tatttggttt taatcttcca aacgaatata aaaaaactgt cacagaggca
    17281 acatattttt gaatatttat aaagtggtag ttttgatttt caaagctacc gccttttctt
    17341 ctatataagc aaacttatcc tcaacaaagt gtaaaacatg gtacatttta agaaaaaagt
    17401 accacagaca caagcaataa aagaaacgcc gagaacctac aaaaacgtag tgcttatgca
    17461 gatttacgac gccttctatt ttctcaaccg tcttaatgac cttgtacaaa agaaattttt
    17521 ctaatatttt ttataatctt atcttgtgat gttttttatg tataaatgac ttaataacaa
    17581 atccaataaa aattatggat attggtataa ttagaacgag gaatttataa tcattataat
    17641 gtgacattat tcttacccaa ctctcagaaa aaatatatcc tatactgatt aatgaagtat
    17701 tccatatggt tattcccacg atagatgata ttacaaaagt tataatattt tgccctgcta
    17761 tacctgctat aaaagatatg tatgttctac ataaagggat taatctacat acacaaacag
    17821 aaatagaaga gtatttatta aaataatctt gagatgcaaa tattcctttc tttgcctttg
    17881 gacatatctc tattatttta tttatgatag atttacctcc ccaatttccc actgtataac
    17941 aaaatatcga accaagtata cctgcaatta tacttaaaat taaaattgtc agaaatccaa
    18001 aatgatttct tgcagcaatt gctccacata aaggtaaaac aacttcgcta ggtaatggaa
    18061 aacaagcata ctcaatcata attaatacaa atacagagat taatccatat tgtaaaataa
    18121 aactattgat tacagacact tacttatcta tctcctttct tatttaaagg ttttaagggt
    18181 acatgttcta aggattctca ccctatattt aaagaatcaa tctctttaaa ctattatatg
    18241 tatttacaca aagcatttat aacatacttt aaaaatagta ccttaaaata agaaacttaa
    18301 taaataaaaa aagattacgt ggctacgtcc tactctccca ggcag
//
LOCUS       DAMJYC010000285        15458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.095, whole genome shotgun sequence.
ACCESSION   DAMJYC010000285 DAMJYC010000000
VERSION     DAMJYC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.095"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            432..1838
                     /locus_tag="PC472_004188"
     CDS             432..1838
                     /locus_tag="PC472_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2729404.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1866..2714
                     /locus_tag="PC472_004189"
     CDS             1866..2714
                     /locus_tag="PC472_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2729405.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLSEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3375..4067
                     /locus_tag="PC472_004190"
     CDS             3375..4067
                     /locus_tag="PC472_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2729406.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4064..4999
                     /locus_tag="PC472_004191"
     CDS             4064..4999
                     /locus_tag="PC472_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2729407.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYMDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            5147..6070
                     /locus_tag="PC472_004192"
     CDS             5147..6070
                     /locus_tag="PC472_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729408.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6054..6824
                     /locus_tag="PC472_004193"
     CDS             6054..6824
                     /locus_tag="PC472_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729409.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6834..7616
                     /locus_tag="PC472_004194"
     CDS             6834..7616
                     /locus_tag="PC472_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729410.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7993..8529
                     /locus_tag="PC472_004195"
     CDS             7993..8529
                     /locus_tag="PC472_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2729411.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8561..8950
                     /locus_tag="PC472_004196"
     CDS             8561..8950
                     /locus_tag="PC472_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2729412.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8987..9373
                     /locus_tag="PC472_004197"
     CDS             8987..9373
                     /locus_tag="PC472_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2729413.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9500..10942
                     /locus_tag="PC472_004198"
     CDS             9500..10942
                     /locus_tag="PC472_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2729414.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            11239..12054
                     /locus_tag="PC472_004199"
     CDS             11239..12054
                     /locus_tag="PC472_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2729415.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            12656..13840
                     /locus_tag="PC472_004200"
     CDS             12656..13840
                     /locus_tag="PC472_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729416.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(14217..15326)
                     /locus_tag="PC472_004201"
     CDS             complement(14217..15326)
                     /locus_tag="PC472_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2729417.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         5520 a         1933 c         2783 g         5222 t
ORIGIN      
        1 gaagcaggaa ccctgtgact ttagtcatgg gaggttcagt ttgggcaagt agtttcttgt
       61 aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc tggtaattaa
      121 gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat caacaattaa
      181 atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta atatggagta
      241 aagctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt attgtaatga
      301 attgttaaag aaaataatta atatagggta aatctatctc aatgaaataa caaagaaaag
      361 agttaatata gagtgaactt attgcaatga atcaacaaag aaaataatca atataaaaag
      421 gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca tcatcttctg
      481 catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta gccgactata
      541 attcttttaa aaagtctcat ttacaggcag atactaaagt cgcatcagac ttttaccata
      601 attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat cgtttttcaa
      661 tttcatgggc aagaataata ccagatggtg aaggagagat aaaccagaaa ggtttagaat
      721 tttataataa gataatagac aaactgatag agtgtgatat tgagccattc gtaactttat
      781 accattttga ccttccattt aaattagtag agaaatataa tggttgggaa agtagagaaa
      841 ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga gatagggtaa
      901 agtattggca acctcataat gaacagaatt taattgttag agtggaggag agaattaata
      961 tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg gattataatc
     1021 tttgtttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa
     1081 ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca gaagatgtat
     1141 atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg catcattatg
     1201 gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatatagtt cctcacatgg
     1261 aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct gtaaattatt
     1321 ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt ggattaagag
     1381 aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat cctaacttag
     1441 aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc ttaaatgaat
     1501 attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga gctaaagata
     1561 tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa
     1621 atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat
     1681 ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa
     1741 atagaactga ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt ttttactggt
     1801 ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt
     1861 ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg
     1921 atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt
     1981 taaataaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagta
     2041 ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg
     2101 catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg
     2161 accacattgc ctttgcagtg agacgtttta aagaaggtat aatgattgaa aatcactatt
     2221 tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg
     2281 tttttaagga ggttcttggt ttagacctat cagaagaaga gattggtaat atagcaggcc
     2341 attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa
     2401 ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt
     2461 cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta
     2521 atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg
     2581 aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat
     2641 taccaagaca agaggagtta tatttgatga ttcatattca taagatttta ggagaattat
     2701 gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact
     2761 acactttaaa tatgaataat tgcattactt aagggggttg tcttaaaatt gattttttta
     2821 attaaatttg aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta
     2881 tacgcctgta ctttagatgt atatctactt tttacatttc tcaacatgca aacccaccat
     2941 tttttgcagt atgttaattg aaataaacca ttgagttaac cctaaattta ttctggactt
     3001 ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt atttcacata
     3061 tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag taaatgtatt
     3121 atcatgtttg ttttattcaa gcacacttat aatatgcttg attttttcac aatgatgaaa
     3181 tatactaatg ggtgttatag gtataagaat agcattacac ttgaagaagt aagtgataaa
     3241 tgagatataa atcctcatat gataaactac tatttcattg aggagcatat tctaagtgat
     3301 gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga atgagtgtag
     3361 aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt
     3421 tgttaaaaaa gagtatctca attgaacaga ttgaagcaaa ctattgccat tctgggagag
     3481 atggactggt tgaactttca aaacaagatt atcagttagt agttctagac gtgatgatgc
     3541 caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct
     3601 taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga gctggtgcag
     3661 atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta ctgtctttaa
     3721 tccgacgtta cacccgtttt aatccaaaaa atgaggagcc acagcaactt tctttcaaag
     3781 gtttgttgat tgaccttgac cagcatacag ttactaccaa tggcgaacct atgttgcttc
     3841 cacccaagga atttggtgta cttctttact gtgcaaggca tcagggtaag attttgacaa
     3901 aacagcagat ttatgaagcc gtgtggggtg agccatatgt atatgatgac agcaatatca
     3961 tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt
     4021 tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa tggacgaaca
     4081 aacgataatt cttttcttac tcttgctgtc gattggtcta ctttttcttt ccatatttgc
     4141 tttttccaag cttgggcata tttacaagcg cctaaaagat atagaggaga tactggctga
     4201 tgtacaaatt ggagacgaaa atcgtaaaat actaattaag ccatgtgatg ttatggctcc
     4261 attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa
     4321 aaagtcggat aaagctagca aacaacttat gacaagctta tctcatgatg ttcgtacacc
     4381 attaacaact ctgattggtt atatggatgc tgtacacagt ggtatagtta tcggacaaga
     4441 gcgggaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg attacattga
     4501 tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa tagaatctgt
     4561 ggaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat tccatgaaaa
     4621 gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaacc tcgacccaga
     4681 tggatattcc cgtgtggtca ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg
     4741 gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg ttgaggatga
     4801 tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt ataagtgtga
     4861 taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc aaatggtgga
     4921 gcgaatgggt ggtcaggttt ccgtagaaag tgagattgga aagtataccg tatttacagt
     4981 gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca cagggggatt
     5041 ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta atgtaagatt
     5101 gaggtggtat gataacttta ataaacaaag gaggctttta ttatttatga ataatttaat
     5161 tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa
     5221 tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg cgggtaagac
     5281 gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa ttaaaatatt
     5341 tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat gtttgattga
     5401 atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct ttgcaaggct
     5461 tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg gtttgccaaa
     5521 caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt tggcgattgc
     5581 tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca atggacttga
     5641 tcctattggt attgcagaag taagaacgtt tattcgtgat ctctgcacag aacgaggtaa
     5701 aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag atgacattgg
     5761 cattattgac catggtattt tgttggagga agaaagtctt gcccaattag aagaaaaaaa
     5821 cagtaaatac atacattttg ctgtatctga tgcagcacag gcagcaaggc ttctggaaac
     5881 ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc tatatgatac
     5941 agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg aggtatcaga
     6001 atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg
     6061 aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga
     6121 tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggaca
     6181 acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac
     6241 caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat aatgatgtat
     6301 taaaaaatct tgcaacagtg ccagtgcaaa aaggaaaact ggctctggca aaattggctg
     6361 tgttgctgtt tatatcattg ttctactctg ttgcagggtt aggagctact atcattggag
     6421 gtttcattgt tggaatagta gagggggttg ctgtaaaatt aggaatgagc attgcttttg
     6481 gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca
     6541 gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt agtatatcct
     6601 taaatattat caattttgaa ccaaataacc caatattaag tgtattacct gctcctgtta
     6661 taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac
     6721 agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag
     6781 cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg
     6841 atataattac tacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg
     6901 ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg
     6961 accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt
     7021 ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa
     7081 ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg ggaaaattaa
     7141 ttgcggttgg aggaattact gtattgttaa gtcttttcag cttcttatgc acatttattt
     7201 tggggttgtt gttttgcaat atcaatatat ctgttacact cgttgtaaaa tccttagttc
     7261 agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt gcttattttg
     7321 gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac ggtttttgtg
     7381 gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc
     7441 tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt gctatgactt
     7501 tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag
     7561 ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata
     7621 aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg
     7681 tctatatgct catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt
     7741 ttgtatttat taaatcacga ctatcataat acttgcaata ggtatagcga caagagagag
     7801 tactgtcata tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata
     7861 atatatttga aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa
     7921 attggcaaat gagatactat ataaatatag aaatagtaat cattactatt attaaaaagg
     7981 gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatttaat ggcaggattt
     8041 gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc aagaaaagaa
     8101 ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa ggaacatgct
     8161 aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa tttaaaggat
     8221 gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc taaagaagct
     8281 agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa aatagagaaa
     8341 gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa aatatttaaa
     8401 agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt aggtactgaa
     8461 gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt atttcaatat aaaagctgaa
     8521 aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt ctaaacaacg
     8581 agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg attacctata
     8641 tgaaaactta aaaaaagata atccaaattt aagcttagga actgtgtata gaaatttagc
     8701 tcagctaacg gaacatggct ttataagaaa ggttagtatt ccagggtatc cagatagatt
     8761 tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag aggtatacga
     8821 tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga cagacataaa
     8881 aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa ggtgtagcca
     8941 agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt gtagtgaaca
     9001 aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa gtggtggtgt
     9061 acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa cagatgcagc
     9121 agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg ttaaagtaag
     9181 tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc ttatgactga
     9241 acaaggtggt caacgcaaat gtctagcagt agatggagaa cctgtagtta aatttgcttt
     9301 gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg gattatggaa
     9361 ggctgaactg taatatttaa ataaattgag tcaaaaagga ggctataaat gtattttcat
     9421 acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta atgtcaatat
     9481 aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat atatttatga
     9541 tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat gccctgtatg
     9601 tacaaaaggt cgtagttatt ttggaaagat aagtactgtt tactatgaag aagatgaaaa
     9661 gatagcagaa gacatagttg aagatgaatc taaattaaat actgaaaaag agggagattt
     9721 aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata tggatataat
     9781 tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacta aactaccagt
     9841 aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac ctttaaatga
     9901 gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac ctatgattat
     9961 agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag aactaaaaat
    10021 agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg aaggaggtat
    10081 attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac caaataaata
    10141 tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa ttggtcaagg
    10201 aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag aaatagcaaa
    10261 agtaagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg aggaaataca
    10321 atccaaagaa gatttaaaga aacttgtaga tgaacttaga gaagtttcag aagggcgtcc
    10381 aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta tagcttatgc
    10441 taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa gtccaaagtt
    10501 gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta gaaaatacat
    10561 agatacacat ggattagata tagatttggt tataacaggt gggcttcgta tatctactga
    10621 ttttgctaag gcaattgcta tgggtgctga tgcagttgct attgcaagtt ctgctcttat
    10681 ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg taggagtagc
    10741 tactcaggat gaggaattga gaaaaagatt acatatagaa aacagtgcta atagagtggc
    10801 aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt ctggtcataa
    10861 agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa tatcaaatta
    10921 tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag atagctataa
    10981 aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt tttagttcat
    11041 aagtcactct tttttttatg aagccaaata tatttatcat tttagcaatg aaaaagaatc
    11101 ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat attaaattgt
    11161 aaaatattct gtgatgtcgt actattaaac taaagattct gtactttaaa gtaaataaaa
    11221 atttaaggag tgattattat ggaaagattg aagatacaag ttttagttga caataatact
    11281 tatattgata gatactttgt tggagaacca gcagtaagct attatattga gattgatgga
    11341 aataggattt tgtttgactc agggtattca gatgtattca tatcaaacgc tgaaaaatta
    11401 aatatagatt taggaaattt aactcatgtt gttttttctc atgggcataa tgaccataca
    11461 agaggtatcc agtttctaga aaataggtat gatttatcta cagttgaact tatttcacat
    11521 cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc attttctgct
    11581 gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa tctaagtaag
    11641 aattgtgtgt ttttaggtga aataccttct ataaatgatt ttgaaaaaag agctaaaatt
    11701 ggtaaatgta aaataggaga tttgtgggaa gatgattatg ttttagatga ttctgctata
    11761 gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag tggaatttgt
    11821 aatatagtag agtatgctaa aaaggtatgt ggagatgata gagttattgg tatactaggt
    11881 ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata tttggataaa
    11941 gaagaaatga gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa agcgaagatg
    12001 aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat atagtatttg
    12061 aaagtatata agatttaagt tgaaggttaa aaagatattt tctatagaaa atatcttttt
    12121 tgttacgttt attttaagag aaatcaatat acttttatat gaaatattgt aaaaaaattt
    12181 atttttaatg agaatatatt ttcattaata aacaatttta ttatgtatta ataatgttag
    12241 aataaatata aataaatcaa agttacttac attacagtat attttttcac aaaatatatt
    12301 gatttattct tttaaagttt gataattcca atgaatgtaa taaaagaagg aattttatac
    12361 tatacgatta ttattatata ataataaaga aaaaattata taataataaa gaaaaaatgt
    12421 ataatattta acattaaaaa agatatttca taaaattaaa aaacaaagtg aatttgaata
    12481 ctatttaaaa aataaaataa atatataaaa aatatttaat ataaaggtgg aaataagttg
    12541 aaaatgtaaa gtcattggaa aaatatagtt gaatttaatg agcaaaaatg ttattgtttt
    12601 atcaataaag tgttaaaata aaaactttca atatcatttt agggaggatg atttcatgca
    12661 aaaaagtgta aagaaagcaa aagtaacggg ctctatgtta gctatttttg gtgttgcaag
    12721 cgttcttttt agttcacatg caggaggagg atttgctaca gggaatcaag aaactcagta
    12781 ctatgtgcag tatggatgga cagcaccact tatggcaatt ttagcaatga ttatacttac
    12841 tgctaccatg agagaagtta tcataatgta taataataac aattgtagga attataagga
    12901 tttattttgt gagctttgga gaccatatcc aaaacttgaa ataatatggg agatatatta
    12961 ctatctaatg gttttaatag ctgtaagtgc tgttatagca ggtgcagcag cagtatttca
    13021 aagtatagga gtaaattatt ttgtagctgt atttattatt ggagttgtac ttcttgtctt
    13081 tactatattt ggtgctatgc ttgtatctaa agcagctaca gcaatgacaa tagctattct
    13141 agtatgtact cttactattt ttattgttgg tataaaagct aaagtaccag aaataactga
    13201 aatattatca aatagaacga gtttcacacc tggctatatc aagccaatac ttaatacttt
    13261 tatttatgca ggttttcaat cggttgttat acctacactt gcaggatgtt caagaccact
    13321 tcttaagaac tcaaaagaag ctactaaggc aatgataatt tcatttgtta tgaatgcaat
    13381 tgcattagga cttgctgtaa ctatgctaat gggatggtat agagaaatta ttgctgctgg
    13441 gcaaacaaca ctccctactc tttatgttgc aggtcaatct ggaaatcata ctatatacat
    13501 aatttataat gttgcattat tcctttgttt aatgtctaca ggagtaacta ctatatttgg
    13561 tcttgtaaat agatttgaag accataaagc actttctttc ctttcttcaa gaatgaaaag
    13621 aagggtattt acagcttgtg caataatggt agtgtcaatg cttatatcac ttacaggatt
    13681 aagtaatatt gttaaatatg gatatggata ttgtggttat cttggtctat tcactatagt
    13741 aattccattc cttacactag gacattataa gaataagaag tttgcaaagg aaaatccaga
    13801 agctaaatgg ccagcggaat taaatgaaaa tgtaaattag atgtagtagc aaaaaatatt
    13861 gctaaagaga atactataga taaggatact atagataata tatctgcatg tttattaaga
    13921 ttatttgcaa aatctaatat taaacaacta taaacaagca atataaaact aagaataaaa
    13981 gataaaaaga gggtgtctca aaatggactt ttagtttata aggcactctt ttttatatga
    14041 agtcaaatat attttcatta ttttgacaat aaaagagact aatcctactt tggatagcct
    14101 cttttttctt taatataaaa tagtataaca gcaaagtata gattgataca tgttttctgt
    14161 agctttctat atattacagt gtttgtgtgg cattggtatt ttaagcaatc tataaattat
    14221 aattctataa ttcttctact aattttagat tctatagccg tctctatttc acttaacatt
    14281 cgttcatttt tagggtcaat aaaagcacat gtatatcctt cttcaccagc tctaccagtt
    14341 ctaccaatac ggtggatgta gtcttctggt gtttctggta aatcgtaatt atatatatga
    14401 cttactccag atatatcaag cccacgagaa gctacatctg tagctattaa gtattgtata
    14461 tctaaatttc taaatgattt cattattctt tctcgttttg attgagcaat atcactatga
    14521 agtttttgac aattatatcc tcttatagca agagcttctt caagattgtc aacccttctc
    14581 ttagtcctac aaaatattat tgccataaaa ggattatctt catctagtac cttacataag
    14641 gcatctaatt tttttctatc tgtagtttct actacttctt gttttatatt attaagagtg
    14701 atttcttctt tttgaataga aactacaaca ggattttttg tgtatctata agctaatttt
    14761 tttacttgag aatttatagt tgcagaaaag catagtgttt gctttttgtt tgaagtctct
    14821 tttaaaatag cttccacttc atttttaaat cccataagca acatttggtc tgcttcatct
    14881 aaaacaaaag tcttcaactt actaaggtct attgtttttc tatttagatg gtctaaaagc
    14941 ctacctggtg tagctataat catatgaata tttcctttta gtttctttat ttgagaacca
    15001 atatcttttc ctccatatgc tgcaagaata cttacatctt tagattcttt aagtttcata
    15061 gcttcttctg ttatttgaat agctagttct cttgtaggag aaagtataag tacttgtata
    15121 tcatttatat ctaatgatat attttcaaat atcggaagta aaaaagctag tgtttttcct
    15181 gttccagttt gagcttctgc tattacatct tttccttctt ttattagttt aatactttct
    15241 ttttgtatag gtgtaggtgt tgttattcct gattttttta gaatatttat tgtattttca
    15301 ctaatcccta agcttttgaa atccattgtt atcttacctt tcatgaaaaa ttttcttttt
    15361 tattaaccaa ttaataatat catatttgta agtattttta gtaaattttt taattattat
    15421 ttatatagct taaaaatata ttaagatact ttaaaatg
//
LOCUS       DAMJYC010000286        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.098, whole genome shotgun sequence.
ACCESSION   DAMJYC010000286 DAMJYC010000000
VERSION     DAMJYC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.098"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC472_004202"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC472_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2729418.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC472_004203"
     CDS             1575..2258
                     /locus_tag="PC472_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2729419.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC472_004204"
     CDS             2255..4915
                     /locus_tag="PC472_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2729420.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQVSVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC472_004205"
     CDS             complement(5096..6277)
                     /locus_tag="PC472_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2729421.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC472_004206"
     CDS             complement(6277..6939)
                     /locus_tag="PC472_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2729422.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7071..7709)
                     /locus_tag="PC472_004207"
     CDS             complement(7071..7709)
                     /locus_tag="PC472_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2729423.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC472_004208"
     CDS             7888..8493
                     /locus_tag="PC472_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2729424.1"
                     /translation="MKIKENVLMLDSTCGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC472_004209"
     CDS             8997..9746
                     /locus_tag="PC472_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2729425.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC472_004210"
     CDS             9743..10507
                     /locus_tag="PC472_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2729426.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC472_004211"
     CDS             10606..11427
                     /locus_tag="PC472_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2729427.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC472_004212"
     CDS             11441..13105
                     /locus_tag="PC472_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2729428.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC472_004213"
     CDS             13110..14984
                     /locus_tag="PC472_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2729429.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVFPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1747 c         2524 g         5219 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 agtttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtacttgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagctgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatggtgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctatatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 atgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttaaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa agatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aatgacattg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaagatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggacgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaga
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt taccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggtaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca tttatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccaactag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aagttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcggttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagt atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtttccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tattttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tgttgctgtt aaacaaggag
    14821 atatggttaa acagggagat gttcttgtta atgttgattt aaagagaata cacgaattag
    14881 gaaagtctac tttgacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatcaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJYC010000287        11141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.122, whole genome shotgun sequence.
ACCESSION   DAMJYC010000287 DAMJYC010000000
VERSION     DAMJYC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.122"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            280..618
                     /locus_tag="PC472_004214"
     CDS             280..618
                     /locus_tag="PC472_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729430.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            640..957
                     /locus_tag="PC472_004215"
     CDS             640..957
                     /locus_tag="PC472_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2729431.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1107..1784
                     /locus_tag="PC472_004216"
     CDS             1107..1784
                     /locus_tag="PC472_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729432.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2112..2879)
                     /locus_tag="PC472_004217"
     CDS             complement(2112..2879)
                     /locus_tag="PC472_004217"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2729433.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3551..4366)
                     /locus_tag="PC472_004218"
                     /pseudo
     CDS             complement(3551..4366)
                     /locus_tag="PC472_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4467..5165
                     /locus_tag="PC472_004219"
     CDS             4467..5165
                     /locus_tag="PC472_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2729434.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            5253..5873
                     /locus_tag="PC472_004220"
     CDS             5253..5873
                     /locus_tag="PC472_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2729435.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            6029..6811
                     /locus_tag="PC472_004221"
     CDS             6029..6811
                     /locus_tag="PC472_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2729436.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6936..7862
                     /locus_tag="PC472_004222"
     CDS             6936..7862
                     /locus_tag="PC472_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729437.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            7876..8325
                     /locus_tag="PC472_004223"
                     /pseudo
     CDS             7876..8325
                     /locus_tag="PC472_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8685..9092
                     /locus_tag="PC472_004224"
     CDS             8685..9092
                     /locus_tag="PC472_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2729438.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            9094..9324
                     /locus_tag="PC472_004225"
     CDS             9094..9324
                     /locus_tag="PC472_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2729439.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(9376..9585)
                     /locus_tag="PC472_004226"
     CDS             complement(9376..9585)
                     /locus_tag="PC472_004226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2729440.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            9714..9917
                     /locus_tag="PC472_004227"
     CDS             9714..9917
                     /locus_tag="PC472_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2729441.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9995..10348
                     /locus_tag="PC472_004228"
     CDS             9995..10348
                     /locus_tag="PC472_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2729442.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10420..11052
                     /locus_tag="PC472_004229"
     CDS             10420..11052
                     /locus_tag="PC472_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2729443.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4106 a         1576 c         1747 g         3712 t
ORIGIN      
        1 ctacatatct aatatgtaat tacaataaaa aatgctataa ttaacatatt attaattcgt
       61 tttattattg tagtatggct atgaaattat gaaaatattt ctctaattaa aaatatgatt
      121 tatccatatt tgtttattgt agatacagac tatacaaaaa taaatagcac ttatttcagt
      181 ataaaaacat atatagtcta tatttagact aaaaaagtat aaatagtcta tataaaacaa
      241 cttaaactat gaatataatc taaagtggag ggataataat tggcaaatat aggaaaaata
      301 ataggagata gaataaaagt ctatagaaca aaattgggat atagtcaaga gtttctagca
      361 gaaaaggcag gtttacatcc aacatatatt ggtcagattg aaagagctga gacaaataca
      421 tctatcaata ttataatgaa aattgctact gctttagaca taccacttga attgttattt
      481 gcaaatatta ttacaacaga aggcgttgat aactctgtac ctttagaatg ttatgaaatg
      541 attaataaat taactgaaaa agaacaaatt gcaatgctag agttaataaa gtgcatcatt
      601 aaatacaaag aaatataagt gagggaaata gtaacaataa tgaattatat aggtaaaaga
      661 cttaaagaag aacggaaaaa agcaaattta acttcaaaag aatttgcaaa tatggttgga
      721 gtatcgcctt ggtatataac tcaaatcgaa agtggtaaaa agaatccaag tcttaaaaca
      781 ttcataaaat ttgtaaatat attaaatatt tctgcagatg ttcttataaa agatattact
      841 tccactggaa aaacatattt agaaaatgat ataaatgaag agttaaaaga cttaaattca
      901 agagaactaa atctgatatc acagattata gacatcataa aaaagaataa aatataattc
      961 tttatatttt atataaaaaa aaaggttatt tgaaataaat aacctttttt tgtctttttg
     1021 tgttaacatt gtcttatagt gctaatgagt actattagac catgttctat ggccttattt
     1081 taatataaaa cgattggaga taggctatga ttgtgattga aggtagcgat aaatttaaga
     1141 ttgcaaaaga atatattgat gtagaatata ctctttttag caaagtaact tttaggtatg
     1201 aaaagttgaa atttaaagat aatgctgaat tggaaaaaat taagatgttt aaatataaaa
     1261 atggctacat ccctaataaa ttaaaccttt cttttggata tggattctct tcttataaaa
     1321 agcaaataat tagagaaact gtagatactt taagattgac agaaattttt tcaagcgaga
     1381 acatagaaga tattaaattt ataaaagatg gtacaaaaaa attagaaatt agcatagaga
     1441 aagttgtgaa atttaaacgt cgaaaaaaaa gaaattatgt ttgttgctat tgccctgata
     1501 tgtatagaga cataaaactc gacaaagaat ctatcaataa gatatacaac agaaaaataa
     1561 aaatagaaag agaagttaat atttttgaag atgaggatgt tataataaac aagaaagtat
     1621 tgaagtttcc aaagtcttgg acaaagaata tgcaaaaata ttggttaagt gaaaataagt
     1681 atcccataca ttctactgta attgatgatg atagatataa atgttgtaat gtacaatata
     1741 caaaaaatag agtgataata ttatattaca tatataacca ttaatataca taattacaaa
     1801 aaaataatga caaatataca taatacaaat atatcttcta ctttagataa aacagttatt
     1861 atgaaaactt actatctata aataatagaa atgctagtat accatctata atctaatgtc
     1921 tagtaggttt taatggcata ttttaaatat gccatagttg atttataaga ttttctatat
     1981 cactttcaaa atacacacta aatagttgct tttcagatat aattgcccaa tttaaatttt
     2041 actatctgaa aagtttttta tatttaattt atatcttcaa aaataccgtc taatatttct
     2101 atataattaa tttaattaat actcttatgt aatagattaa gtactgaatt taactcttta
     2161 aatgatattt gccctggtgc tgaaacactt tttgcagctc caaaagtcaa tgcagaacca
     2221 aatatttcac cacacaatct acttataaca cccattcctg acattgacat tgttattatt
     2281 ggtctatctg cataaatttt aaacatttca tttgtagctt caagtagcac taacacatct
     2341 ttttcatttt gcggcataac agctattttg ggtaaatctg cgcccaattc ctgcattcta
     2401 cataaacgag atacaatctc ttcttttttt ggagttttat taaaatcatg attagatata
     2461 attactttca cttctttttt atgagcaaaa ttaactactt catctatgac ctcatctccc
     2521 ataaataact ctacatctat taaatctact aaacctgtat tagaaatttc tttattcaaa
     2581 gtagtataat aatctcttga tattaatttt tctccccctt ctactacact tctaaatgtg
     2641 aataataaag ggatatcatg tatataactt cttaattcat agagaacttc cttaacttct
     2701 ttaatatttt ctacattctc aaaaaaatca actcgccact ctataatatc caaacatgca
     2761 tcttttaact ctttagcctc ttttataata tcttttttat ttttcccaat aattggtaca
     2821 caaattttag gtctaccttc tccaatagta atatttttta cttgaacttt tcttttcata
     2881 ttctcctcca taatcagaaa ttctatagga ctttaaattc tattttctgc acaatttaat
     2941 tatacattaa aaatactata cattataaaa atactaattt aaaaataatt tttattctaa
     3001 ttcttaaaac aatcccacat attttagaaa aggtactcta taatatacaa acacttcttt
     3061 tttacctaaa tttattttaa catcctataa ttttcacaat attctttaaa ttttactcat
     3121 ataaacaata ttattctgga tgctaataga attaatctat aaatcatagt ataattatgc
     3181 agaaataact cgaatttcac tacttatgaa gagattgaat attaatgaat ctaaatcatt
     3241 tgcattactt aagagttttg actaaagttg aacaccatac aaaagtagct actcaattgt
     3301 ttatacccca accaagctta agtaatgtta tatagattct cgaaaaagaa tttggtatct
     3361 ttttatttga aaaacttata ttaaatttga ttatcttttc ataagttggt aaatgtcgat
     3421 atatcctgta tctacaagga gtatcggcat tttctttttg gaagatactt tgcataagtt
     3481 ggcatttaac cgtataagaa tgccatcaaa agtagtaaat aagtagtaaa ctctagtcat
     3541 actactagga ttataaccgt tccatttcag ctctcgaaga actaaatgaa ccatgtgcat
     3601 aatagccaag cgtcatagta atgtttttat gccccataat atattgcaat atattaggac
     3661 tcattccttt gttagccata tttgtacaga gtgtatgcct aaaggtatgt ggcgtaatct
     3721 ttggtagctt gtcctcatgg gtcttattat acttcttgac taacgctctt aacatgcttt
     3781 gataatgagt tgcttgtttt ggtaatccat cttgtttaag aaataggaag ttctggtaac
     3841 catcaacaat gattggttct gctttagcgt tcttacgccg ttccacgata cgttcaaatg
     3901 attgataagc tctttcagtc attggaattt ctcgcttacc ctgtgtggtc tttggaactt
     3961 ctacataata gccaatctta gtatcactca acaattgatg gtctaccata atggctttat
     4021 ttttcatatc caattctaaa gtcaaaccgc ataattcaga aatgcgtagt cctgtttcta
     4081 agaggataac aacatcatca taatatttac aataagtctt atcgctcccc ataaaggata
     4141 ataagcccat ttcctgttct ggtgttagag cgatttttgg ttctgttgta tcttccaaaa
     4201 catcacttaa ctaaaaatta aatggattct ttctaataca gtcgtcttga attgccatga
     4261 aaaacgacgc tttcagtgag cgcttataat tgtcaatgga tttataagca cacccatctt
     4321 cactcattcg gatagcccag cttttagcgt ctgataattt aaccatatca atgctttttg
     4381 cacctagtgg gtcattgata gtatctatat tttgatttat aatttataca tttttttaaa
     4441 aacagacttt taactgtaaa gaatctatgt ataaacttag ctatgttgtc cgtgtatcta
     4501 caaagaatct tgacgagcta aaaaggcgtt gtgatgaagt caaagacttt tacgacgact
     4561 tatcaatcaa gctcgtgcgt ccctttgggg atatgttggg attgcatggc gaatttattc
     4621 ctgtcagcaa acgttacatc aatgattata ttcaatatgt cacttcggat tttttggctg
     4681 gacttggttt tggagcgaca caaacccttg gcgaacatga ggatatctat cttggctaca
     4741 atctggacac tggaaagaat gtctatctaa aacctgccct tgctagtcaa ggggtaaagg
     4801 gttctattac caatgcgtta gcgtctgcgt ttatcggttc attgggtggt ggtaaattat
     4861 tcagtaacaa tatgcttgtt tactatgcgg tactattcgg cggacaggcg gtcatagttg
     4921 accctaaagc agaacgcggc aaatggaaag aaacgctacc agaaatagcc catgagataa
     4981 acattgtcaa tttaaccagt aatgatgaaa acaaaggatt gcttgaccct tacgtcattt
     5041 tgagccgtaa aaaggattct gaaagtttag cgattgatat cctaacattc cttactggta
     5101 tctccagtcg tgacagtgat aagtttttgc agaaaaatga caactggaaa ataataatgg
     5161 aatagtttct atgtaaatat atttttacta tcttacctaa ctaattgaaa gatgatataa
     5221 tttaatcaaa gatttggtaa ggagggatga atatgcattt aattgatttt atgaatgaag
     5281 atattaaaaa gaagcttgtt aaaaaaacgt ataaaaagaa agagactatt ctttttgctg
     5341 agcaagaaaa tgaatatgta attcttatga ttgacggtat tgcagaagca ttcatcttaa
     5401 atgtgaaagg aaacatctca acgattcatc tttatagaag tggtagtttt tttggagaaa
     5461 tggaacaatt taatgatggg aaaaaacccg ttgaaattgt cgctttcaca gattgtaatg
     5521 tatataaact tcatagagat gattttttaa actggttaag gggagatttt gaagctacaa
     5581 aatttctgat tcgagaaata tctagtaaat tagttgtaaa tgctgaatta attgaagaat
     5641 tatcatcttt aagtgttaaa gaacgacttc tgaggtgtat atccttacac tattatagaa
     5701 gtgaattaaa tctgcttacc aaaaaacagc ttgctagcga agtaaacgct ccaattagaa
     5761 gcattaatcg tgctataaat gaatgtgtac aagaacatct aattgcttat gaaaacaaaa
     5821 gatttagtgt aattaatcag cgagaggtat taaagaatct tcctacaaca taagtataat
     5881 ttcaaaaaag tattgaataa taattattca atgctttttt atttcgggtc aagtgaccta
     5941 ttttttttta tttcttcatt atataatact cctatgcata attatgttgg attgatagct
     6001 aataaaagac agtgagaggt gttatattat gactaaaaca caacagaaaa tgatacaaaa
     6061 cgtattagaa ttttcaaaaa gagatagtag gataatagga cttgcaatta gcagttctta
     6121 tatcacaaaa tcgttagatg aatattctga tttggatttt ctgattgtag cagatgataa
     6181 atcctatgaa gaagttatcg aagagagatt acttattgtg gaacagtttg gaacattagt
     6241 ttctgctttt actggcgaac acgttggaga acctcgattg ataatttccc tctatgattc
     6301 ccctgttctt catgttgatt ttaagtttac aaccttagat ggtttactag atagggttga
     6361 agattcagct attctctatg aagaaaacaa ctgcataaca gaaatttatt ctaaaaaaga
     6421 agcatacttc ccaactccag acttacagtg gattgaagat agattttgga tttgggttca
     6481 ttacgcttgc actaagattg gacgtagaga actatttgaa acaattgatt ttttatcttt
     6541 tttaagacaa tcagtacttg ccccattaat acagcttctc ttaggtaaat tacctcgtgg
     6601 agttagaaaa attgaattgg acgcacccca atatattgaa gcattagaaa agacggttgc
     6661 taaccatgaa tataaaagct gtgttaatgc attgcaggct actatcaacc tgtatcttga
     6721 gttaagagag gaattatcac aaagtcctat tgtccgaaag gttgaagctg aaagggttgc
     6781 attacagtat tttcaagatt taatcaaata aacccaaagt aaaaaaatat tgaatagtaa
     6841 atattcagtg ttttttcatt tttggtcaaa tgacctagat tagttgttgt aacaattcta
     6901 taattatttt gaaagtaaca gtatggagga taactatgag tgatatttta agtagaatta
     6961 caaataaaat tggggataag aatatagttg ataagttatc agctctatca aaatcagatt
     7021 tgaactcact tctattagaa gtgtttgata gacaagcaaa tactttaact gcaactgata
     7081 ttctaaagtc ataccaacta aatagattta ctatccctag cggtatagat ccagaagaaa
     7141 ttcatgcttt ggaatcaaaa ttattgagaa aagcattcaa tatggatatc aagacgatta
     7201 tgctttctcc ctctgcccca ttaggtagct gtagcgtttt tggctccgta gatcaatata
     7261 acgtagtaag tgctctacga gggacagaaa cacttgcaga cccctcaaat atgttggcaa
     7321 taattattgc tgataaatta aaaagaaaag aagaaaataa taccatacct atccacatgg
     7381 ctactactgc tagggtaata agagcacaaa attttgtagg taaaggttta tattcacatt
     7441 ttggcttata ttgtatggtt tcatctggta tagatacagg gtcttacact tgtgaaaaaa
     7501 tgttgttaga aaagcactta aactattata aagatatatt aagtgaaata gaaaatgttc
     7561 gtttctctat tattctacgc aaaagaaacg gatacaaaga taatgacgga tttttcgaca
     7621 gaatggttga aactatcaaa cttatttttc ctaatataga gttatcaatt cataatgatg
     7681 atgttgataa taattattat aaaggtatca atttcaaatt atcggtcaaa caaggaaatg
     7741 aaaccgttga aatagttgac ggtggatttg ttgactggac ttatcaaatg cttggaaata
     7801 aaagagaacg ctgtttgata agtggtattg gcttagaaag atttcttgta gctgtttcat
     7861 aaaaggagaa gaaaaatgga acttatagag tggaattatg cttatattac cgatttagtt
     7921 gagtttgcga atgataaaga aattgcaaag aatcttcgtg acaacttccc ccacccttat
     7981 agggagcaaa acgctagaga ttttgttagt ttttgtttgg caacttcaga tgttaaacaa
     8041 cttagctatg ctatttttta tcaagaaagg gctattggaa gtatcagcct aacatttgaa
     8101 gaagatacct ctattaataa aaatgctgaa ttaggttact ggcttgctag gaactattga
     8161 ggcaaaggga tagcaacaaa tgcagtaaaa gaaatatgca aaattgcctt tgaaaaatac
     8221 aaccttaaca aaattcatgc taatgtatat tcctacaata gagagtcaag taaagtattg
     8281 gaaaaatgtg gctttaagat tgaagaatac tgtcacaatt cataataaag aatagaaaca
     8341 tgattgattg ccatgtatat agtttaattc gaccgcaata gcatttatta gaattaacct
     8401 actttttaat aacagaataa gtttttcccc tctattaatt tcttatgcat ctttgtattt
     8461 ttatgaaaag ctagccaatg catatttcca tacatcaata tggtggtggc ttacaaccaa
     8521 tataaattaa atacgtttta aagaatacac atttttgccc tataggcaga gtgtgtattt
     8581 ttttctcttt tttttgaaat ttatcgaaga tttaacgccc tcccaccgtg ataggagtaa
     8641 gcaaggttat ttaactcctt gcgatggaaa gagggtgaaa tcttatggtt gatgaaaaga
     8701 caattgaaca tcaattcgac agtttctgta aaacagtttt gcgtaactat gctagagata
     8761 tttatgacga aaataaacgc agaaatgatt atttggtatc tctcgaatct ttgtcacttg
     8821 cagaattaag taaattgagt attttagatg attatgattc taactacatc tgtatggttt
     8881 cttacgacta caacatacga attgaagatg tattaatggc gcaagcaatc ggaaaactta
     8941 caaaaagaaa acaagatata attctacttt cattcttttt caacatgaca aatgctgaca
     9001 tagcaacatt gatggattta gcagaaaata ccatacatta tcacaaaaca aatgcactaa
     9061 aagagttgaa agaactcatg caggaacatt gaaatgaaac ctactaaaga agtattacct
     9121 tttacaatta ttttacttgc tgtacatggc gatgtaaatg caataaacgt aatacttaaa
     9181 cattttgaac actatatcat tcgtctttca cagaaaactt tatttgatga atacggaaat
     9241 ccctatattc atgtagaaga agaaacaaag cgaatgctag gaacaaaact aatcactgct
     9301 attttgagat ttaatttgtc ttaatctcgc cctctctcca tgtatcaagc tatttggtac
     9361 atggagaatc actaattatt tgtctataaa agcccacaag ctattgtgat tttttatagg
     9421 caaatcaatg ccgactgttc tttgacaact gaataaagta gatagatacg tttgtgatac
     9481 agatagccaa caaatactat acgccatgac cttaatcaag agagctacta tatagtgatt
     9541 tgtgaataac ctttggaacg gttgttttgc cgtaacctgt accacagtat aatgatacac
     9601 ctgtatgaca cggttctgtc cataagaatg ggattggttg aaataccaat ggagcttatc
     9661 aaagccatct atctacttct gaactacgaa catagaaagg agtgatacat tatatgaaca
     9721 atactgatgt acctatttgg gaaaaatata ccttgacgat tgaagaagcg tcaaaatact
     9781 ttcgtattgg agagaaaaaa ttacgtaaac gtgctgatga aaaccttgat tctggctggg
     9841 tcattatgaa tagcaatcgc attcaaataa agagaaaaca atttgagaaa atcgttgata
     9901 cacttgacgt tatctaatga aattgagcct tatatgttat aatattcata tgacgtatca
     9961 agctcaattc ggttattgag aggagcttct tatcatgtca actgaaaaaa gacgagataa
    10021 taaagataga attttaagaa ctggagagag ccagagaaaa gatggaagat acgcatataa
    10081 gtatgtatat tcatttggta aaccacaatt tgtttactct tggaaacttg tacctactga
    10141 taaaccccca aagggaaaac gtgatgatat atcattaagg gataaggaaa aagcaataca
    10201 aaatgacctt gatgatagca ttgacactat tggtaagaaa atgacagttt gtcagttata
    10261 cgctaaaaag aatggactaa gaaagaatat ccgacttaac actaaaaaag gacgccatta
    10321 tcttatgaag attcttaaca aggagtgatt ctgtgaagag atgtattgta gtagtggaat
    10381 agtcaaaaac ataatatata ttaaaggaga gatttaaata tgactattcc aagttcaaat
    10441 aaggtatatc caagaagtaa tgattatcaa actgtatatc ttaaaaatgt aataacaagg
    10501 gaaaatatag aagttggaga ttacacaata tataatgact tttataatga cccaagagat
    10561 tttgagaaaa acaatgtatt gtatcattac cctataaaca atgataaatt aataattggt
    10621 aagttttgct caatagcatg taatgcaaaa tttctaatga ctagtggaaa tcactcaatg
    10681 aaatcaaaat ccacatatac ttttcccatc ttttatgaag aatggaaact cgatatcaac
    10741 catataacgg acgcttggga caataagggt aacattataa ttggaaatga tgtgtggata
    10801 ggttatgatt ctataattat gtcaggtgta aatattggtg atggtgcaat tattggaaca
    10861 agagcagtag ttactaatga cgttccaccg tattctattg ttggaggagt acctgcaaaa
    10921 attataaaaa agagatttaa taatgataca atttcaaaat tattagaaat taaatggtgg
    10981 gattggccat ataaaaaaat tcaatcaaac attaagcata ttcaatcagg aaatattgat
    11041 aaattagttt aatattttat aatttattta tattaaactt tactttaata atcattttat
    11101 aagagctttt tcaaaatttg tatattattt taatatatat a
//
LOCUS       DAMJYC010000288        10551 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.128, whole genome shotgun sequence.
ACCESSION   DAMJYC010000288 DAMJYC010000000
VERSION     DAMJYC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.128"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            83..298
                     /locus_tag="PC472_004230"
     CDS             83..298
                     /locus_tag="PC472_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDF2729444.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            315..587
                     /locus_tag="PC472_004231"
     CDS             315..587
                     /locus_tag="PC472_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDF2729445.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            589..738
                     /locus_tag="PC472_004232"
                     /pseudo
     CDS             589..738
                     /locus_tag="PC472_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            799..1140
                     /locus_tag="PC472_004233"
                     /pseudo
     CDS             799..1140
                     /locus_tag="PC472_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1265..2002
                     /gene="erm(B)"
                     /locus_tag="PC472_004234"
     CDS             1265..2002
                     /gene="erm(B)"
                     /locus_tag="PC472_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDF2729446.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2148..2288
                     /locus_tag="PC472_004235"
                     /pseudo
     CDS             2148..2288
                     /locus_tag="PC472_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2307..3215
                     /locus_tag="PC472_004236"
     CDS             2307..3215
                     /locus_tag="PC472_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDF2729447.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3212..3754
                     /gene="sat4"
                     /locus_tag="PC472_004237"
     CDS             3212..3754
                     /gene="sat4"
                     /locus_tag="PC472_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDF2729448.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3859..4191
                     /locus_tag="PC472_004238"
     CDS             3859..4191
                     /locus_tag="PC472_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2729449.1"
                     /translation="MARKYDHEYKVQAVKLAKEIGGAKAAKELGIPEGTIHTWLKAVR
                     AGTLDIGDGAHTPESAMSLAEELAMLRKRVKDQDKEIRRLKEENEFLEEASAFFAASR
                     RKSARTKE"
     gene            4188..5096
                     /locus_tag="PC472_004239"
     CDS             4188..5096
                     /locus_tag="PC472_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2729450.1"
                     /translation="MKFIAIKTEDGVIKGKLSFYCRMLGVSRQGFYKYLAIKDRPWKY
                     QDLADAMRAINSEDECNDTYGRIRMYQALLLKNLEGLKIPSERTVYRVMDEIGLSHRP
                     KRKPNGITRADREARKSDDLLKRDFKSNKPLEKCVTDITEIKAKDGKLYVSAIFDCFD
                     SSVLGLAMETNMKATLCEHTLDNAYLAYPNLRGAILHSDRGTQYTSETYRKALAKYGI
                     IQSMNSAGGRCHDNARCESMWARMKSELLYDRYNTETMTTEELKVLIWRYFISYWNNR
                     RICSANGGLPPIIKRQRYYQSLDLAA"
     gene            5214..6008
                     /locus_tag="PC472_004240"
                     /note="aph(3')-IIIa"
     CDS             5214..6008
                     /locus_tag="PC472_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDF2729451.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6284..6856
                     /locus_tag="PC472_004241"
     CDS             6284..6856
                     /locus_tag="PC472_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2729452.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            6829..7236
                     /locus_tag="PC472_004242"
     CDS             6829..7236
                     /locus_tag="PC472_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2729453.1"
                     /translation="MVLNRFCRLRNEYRNFRVDRIKSICIEEELCQSHDGSLEQILKQ
                     MLSYKKLYNVILRAEKGETYNSIKNRYSLGFLEETDLGSKMEIEFQTDSFEILSKQLI
                     EYGSGIEIVQPDELKCITRKHLAQITNHCLNLI"
     gene            7347..8006
                     /locus_tag="PC472_004243"
     CDS             7347..8006
                     /locus_tag="PC472_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HDF2729454.1"
                     /translation="MKPKIIMHTQISLDGRIKGFDNPEVYYQVAGGIHSDAVLFGSNT
                     VFTAFEKYPAETEADFEKIITSPEDPRPIGVIPDSRGILRSLHCLRNLGYLKEIVILV
                     STATPKEYLNYLEERHYPYIVSGNDHVDYEKAFQILHEQYGCKYMRTDSGGGLTNKLL
                     ENGLIDEISLVISPCFVGNKEKQLFDNLLLSEKVNLELQGTENAEHGCLSLRYAVKNK
                     D"
     gene            complement(8727..10331)
                     /locus_tag="PC472_004244"
     CDS             complement(8727..10331)
                     /locus_tag="PC472_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
                     /protein_id="HDF2729455.1"
                     /translation="MYQNYTINQLCLPIDLEIKLEENDFAHAIVQFVDSIPDEVFLPY
                     YQSMGRPQYHPRMLLSIILCAYIQGVYSGRQIQNMLIDSIRMRYLSQEQFPNFRTINR
                     FRVHPIMNDILDHSFVQFRELLVQSGLISGSALYIDGTKIEADANKYSFVWKKSILKY
                     KDSLDQKALENYQQMVETKILPELIDDLKDELSIEEIERIRQSLEEKENQLIEAIDQT
                     ETVEERKLLRKEKSEVHKQKKRFDDFAQRKMSYEEQLVIMGVRNSFSKTDHDATFMRM
                     KEDHMLNGQLKPGYNIQLATENQFALAYDCYPNPTDTRTFIPFLEKIESKIGLPENIV
                     ADAGYASEENYCYVLDETESTPIIPHQGYLKEKKRAHKQNQFHRDNWTYNELDDYYIC
                     PNQKRVVFSHYSQRKDRNGFIRNFKIYECEDCTGCPLREKCTKAKEGKNRRLNINPTW
                     EYIKIKANEKIESKETASYYAQRKIDVETVFGNIKQNMKFKRFHVRGAEKIFKEMGLV
                     FLAHNFRKLVTRVRKYEGKTIIQNQI"
BASE COUNT         3487 a         1762 c         2234 g         3068 t
ORIGIN      
        1 tcacaagtga ttaatcacaa atcacttgtg atttgtgatt gttgatgata aaataagagt
       61 aagaagaaat agaaagaagt gagtggttgt gggaaattta ggcgcacaaa aagcaaaacg
      121 aaatgataca ccaatcagtg caaaaaaaga tattatgggg gataagacgg ttcgtgttcg
      181 tgctgacttg caccatatta taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
      241 agttatggat aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa
      301 ggagtgaaaa tgaaatggca gttacttatg aaaaaacatt tgaaatagag atcattaacg
      361 aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaatcat gaattaaata
      421 aaaatgactc tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg
      481 taaatctttt tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa
      541 tgaatcgcta ttcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata
      601 gtcaatttta ctgacaaaca atttgaaaat cgcttaaatg ataatttaga agaattggtt
      661 caaggaaaaa aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca
      721 gggaaaacga gtttgcgaag aagatgattg agcaggcagg aacaaagtga taaattcaaa
      781 ttgtgaggga aaatatagat gaatcaagga agaattattg tgattacagg ctcaccgggg
      841 acaggaaaaa caacaactgc gtcaattgtc gcaaaggaat cagatatgga taaatcagtg
      901 catatgcaca ccgatgactt ttttcattac ttaagcaaag gcgcaatacc accacatttg
      961 ccagaatcca acaagcaaaa tctggttgtc attgaagcct ttttagaggc tgcaaagcga
     1021 tatgctcgtg gcggatatga tgtaattgta gatgggattg tcgggccatg gtttttggag
     1081 ccatggaaag ctcttgccca agaagattac gaggtacact atattgtatt aagagcgagt
     1141 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
     1201 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga
     1261 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     1321 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     1381 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     1441 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     1501 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     1561 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
     1621 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     1681 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     1741 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     1801 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     1861 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1921 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1981 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
     2041 ttactaaagg gaatggagat aaattattag gtatactact gacagcttcc aaggagctaa
     2101 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     2161 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     2221 cgttttatgg ttgttcaaat tatcctgaat gtaagtttac tttagctgaa cattttagaa
     2281 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
     2341 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
     2401 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
     2461 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
     2521 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
     2581 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
     2641 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
     2701 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
     2761 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
     2821 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
     2881 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
     2941 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
     3001 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
     3061 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
     3121 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
     3181 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
     3241 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     3301 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     3361 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     3421 tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg
     3481 aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg
     3541 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     3601 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     3661 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     3721 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtc
     3781 tggtgtggta taaaaaaagt tgacatctta ttctcaagga tttcatgata gaatcaagag
     3841 aataaggagg tactcaaaat ggcacgtaaa tatgaccatg aatacaaagt acaggcagtt
     3901 aagttagcaa aagaaatagg tggtgctaaa gctgctaaag aattaggtat cccggaggga
     3961 actatccata catggctgaa agcagttaga gcaggcacat tggatattgg cgatggtgca
     4021 catactccag aaagtgcgat gagtcttgct gaggagcttg cgatgctccg caaacgcgtt
     4081 aaggatcaag ataaagaaat ccggcgtctg aaagaggaaa atgaatttct cgaggaagca
     4141 agtgcttttt tcgcagccag ccgtcggaag tcagcaagaa ccaaagaatg aagttcattg
     4201 ccataaaaac ggaagacggc gtgattaagg ggaaactctc attctactgc cggatgctcg
     4261 gtgtcagccg ccagggtttt tacaaatatc ttgctattaa agatcgtccc tggaaatatc
     4321 aggatctcgc tgatgctatg agagcgatta attctgagga tgaatgcaat gatacctatg
     4381 ggcgtattcg catgtatcag gcacttctcc ttaagaatct ggaaggactc aaaatcccca
     4441 gtgagcgaac tgtctacagg gttatggatg aaataggcct tagccatcgg ccaaagcgta
     4501 agccaaatgg tattaccaga gctgatcggg aagcgcgtaa atcggatgat cttctgaagc
     4561 gagatttcaa atccaataag ccacttgaaa aatgtgtaac tgacatcaca gagatcaagg
     4621 ctaaagatgg aaaactgtat gtttcagcta tctttgactg ctttgattcc agtgtccttg
     4681 gtctggcgat ggaaaccaac atgaaagcaa ctttgtgtga gcataccctg gataatgcct
     4741 atcttgcata tcctaatctg cgaggcgcca ttttacactc tgacagagga acacaatata
     4801 ccagtgaaac ctatcgtaag gctctggcaa aatacggtat tattcaaagc atgaacagtg
     4861 ctggtggcag gtgccatgat aatgcccgat gtgaaagcat gtgggccaga atgaaaagcg
     4921 agcttctcta tgaccgctac aatacggaaa ctatgaccac agaggaactg aaggttctca
     4981 tttggagata tttcatcagt tactggaata acaggaggat ctgctctgct aatggtgggc
     5041 ttcctccgat catcaagcgc cagagatact atcaatcttt ggacctggct gcatagtcag
     5101 tgatatcttt gagataaatg tgtcaaccaa tattgacaat atcagttcgt cttgttataa
     5161 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     5221 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     5281 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5341 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     5401 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     5461 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     5521 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     5581 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     5641 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     5701 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     5761 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     5821 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     5881 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     5941 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     6001 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     6061 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     6121 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     6181 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     6241 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     6301 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     6361 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     6421 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     6481 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     6541 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     6601 atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt
     6661 gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac
     6721 tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc
     6781 ggaaagcaga atgatagagc ctatatcact ggggttttat gaacagaact ggtacttaat
     6841 cggttttgca ggctaagaaa tgaatatagg aattttcgtg tggatcggat taaaagcatt
     6901 tgcatagaag aagagctctg tcaaagccat gatggctctt tggaacaaat attaaaacaa
     6961 atgctttcat ataaaaaact atataatgta attcttagag ctgaaaaagg ggagacatac
     7021 aattctatca agaacaggta ttctctcggc ttccttgaag aaaccgattt gggaagtaaa
     7081 atggaaatcg aattccagac agattccttt gagatattga gcaagcagct gattgaatat
     7141 ggatcaggca ttgaaatagt ccaacctgat gaattaaaat gcataacccg taaacacctg
     7201 gcacaaatta cgaatcactg cctaaacttg atttagagaa agcgattggt ctgttatgta
     7261 ttttttgtaa ggtttcatat cccctgctga catacagctg tcagtgtatt atgatattct
     7321 actatcaaaa aggaggattg aaaaatatga aaccaaagat aattatgcac acacaaatta
     7381 gccttgatgg ccgcattaaa ggctttgata atccggaagt ttactaccag gttgccgggg
     7441 gaattcattc ggatgcggtg ctgtttgggt ccaatactgt ttttactgca tttgaaaaat
     7501 atcctgctga aacagaggct gattttgaga aaatcataac aagcccggag gaccccagac
     7561 caataggagt catacctgac agcagaggta tcttgagaag tcttcattgc ttaagaaatc
     7621 ttggatatct gaaggaaatt gtcattctgg tatcaactgc cactccgaag gaatatttga
     7681 attatttgga ggaaaggcat tacccatata ttgtgtcagg aaatgatcat gtggactatg
     7741 aaaaagcctt tcagatcctg catgagcaat atgggtgtaa atacatgcgg acagacagtg
     7801 gcggtggatt aaccaacaaa ttattagaaa acggacttat tgatgaaatc agtcttgtga
     7861 tatcaccatg ttttgtaggt aataaagaaa aacaactatt tgataacctt ctgttatctg
     7921 aaaaagttaa tttggaattg cagggaacag agaatgcaga acatggctgt ctgtcattgc
     7981 gctatgctgt gaaaaataag gattagtgca gcatacttta aaaagtgtct gttgggacag
     8041 tgtcctaaag gaagcataag ctcatggcta gcggcggtgt taaagtaacg gcactcggcc
     8101 ccaatatata gttgatatgc ccctaagcct ttgagggcaa ggcggtcaac cgcgcctttt
     8161 ccggtccgca tacagtcgtt caatgagggt atcaagctcg gcaatgcggc ggtgcttctc
     8221 ggtggttcgt ttcttcgccg ccgttttcgg ctaattctaa ttgatccgaa ctgtaaggat
     8281 aagcaattat ccggggctag ttaagtatct tagagaggaa gataaagata agtaaaccaa
     8341 ttttaaagtt gtaccgaata aagccttcaa gaggacaaca tgcttttgaa ggctttattg
     8401 cttacaatta cttggattgt aacgcaaaag caggcaccaa atcaatgaaa aatagcctat
     8461 ccaggcgact gtttcagaaa tatgggcttc cgccaagggg ataccagtgg ggtgcgacat
     8521 cttttttgcc cactcaaggc atgtggagtg tgtggcgttg ttgcgtagaa agtaaggctg
     8581 gagtccttat atctggctga gcttaatctc tggtaatttt gagggggctg acccattagt
     8641 cccttttgaa ccaaaaaatc gaaggcaccc gtgaaagatg tcttcgattt ttgttttttt
     8701 tggatgaaat atagaggatt ttccccctat atttggtttt gtatgattgt ttttccctca
     8761 tatttcctta ctctagttac taatttccta aaattgtgcg caagaaatac aagtcccatt
     8821 tccttaaaaa ttttttcagc tcctcggaca tggaatcttt taaatttcat attttgttta
     8881 atgtttccaa aaactgtttc gacatctatc tttcgctgcg catagtatga ggctgtttct
     8941 ttgctttcta tcttttcatt cgcttttatt tttatatatt cccaggttgg attaatattc
     9001 aaacgtcgat tttttccttc ttttgctttg gtacatttct ctctcaatgg acatcctgta
     9061 caatcttcac attcatagat tttgaagttt cgaataaatc catttctatc tttacgttgt
     9121 gaataatgtg agaacactac tcttttctga ttgggacaaa tatagtaatc atccaactcg
     9181 ttgtaagtcc aattatctcg atgaaactga ttttgtttat gggcgcgttt cttctctttt
     9241 aagtaacctt gatgcggaat gatgggggtg gattctgttt cgtctaacac ataacaataa
     9301 ttttcttcac ttgcataacc ggcatctgcg acaatatttt ctggtaaacc tatttttgat
     9361 tcaatctttt caagaaatgg gataaatgtt cgtgtatctg ttggatttgg ataacaatca
     9421 taagcaagag caaattgatt ttctgtcgca agttgaatat tataacctgg ttttagttgg
     9481 ccattaagca tgtgatcttc tttcattcgc ataaaagtag cgtcatgatc cgtctttgag
     9541 aaactattgc gtacacccat aatcactaat tgttcttcgt agctcatttt tctttgggca
     9601 aaatcatcga atcgcttctt ctgcttatga acttcagatt tttctttacg aagtaatttt
     9661 cgttcttcta ctgtttcggt ttggtcaatc gcttcaatta attgattttc cttttcttcc
     9721 aaagattgac gaatcctttc aatctcttct atagaaagtt catcctttaa atcatctatt
     9781 aactcaggta agatttttgt ttccaccatt tgttggtagt tttcaagggc tttttgatca
     9841 aggctatcct tatactttaa aatacttttt ttccaaacga aagagtattt attcgcatcc
     9901 gcttcaattt ttgtaccatc aatgtataga gcactgccac tgattaagcc actttgaacc
     9961 agtagctcac gaaattgaac aaatgaatga tctagaatat cgttcataat cggatgaacg
    10021 cgaaaacgat taatagtacg aaagttaggg aattgctctt gagataagta gcgcatccga
    10081 atactatcta tcagcatatt ttgtatttgg cggcccgaat aaaccccttg aatataggca
    10141 caaagaataa tggaaagtag catacgagga tgatattggg gtctacccat agattgataa
    10201 taaggaagaa agacttcatc gggaatagag tcaacaaact gcacaatggc atgagcaaaa
    10261 tcattttctt ctaatttaat ttcaagatct ataggcaaac aaagttggtt tatggtataa
    10321 ttttgataca tgataaagca cctctctcaa atggattaat cacctatatt atagcagagt
    10381 gtgctttatt tttgtattca ctttcaaaag ataaatagag gtctttccac tactatctaa
    10441 atggaaagac ctctattttt catataggag ctactttttg gtcagcccta tatattaatc
    10501 acaaatcact tatcacaaat cacaagtgat taatcacaaa tcacttgtga t
//
LOCUS       DAMJYC010000289         7180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.161, whole genome shotgun sequence.
ACCESSION   DAMJYC010000289 DAMJYC010000000
VERSION     DAMJYC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.161"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..194
                     /locus_tag="PC472_004245"
     CDS             <1..194
                     /locus_tag="PC472_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HDF2729456.1"
                     /translation="EKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTITA
                     HSENNIIIFKVIIPIVGNL"
     gene            455..1555
                     /gene="vanG"
                     /locus_tag="PC472_004246"
     CDS             455..1555
                     /gene="vanG"
                     /locus_tag="PC472_004246"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2729457.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1552..2358
                     /locus_tag="PC472_004247"
     CDS             1552..2358
                     /locus_tag="PC472_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2729458.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            2376..4514
                     /gene="vanT"
                     /locus_tag="PC472_004248"
     CDS             2376..4514
                     /gene="vanT"
                     /locus_tag="PC472_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2729459.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            4828..5448
                     /locus_tag="PC472_004249"
     CDS             4828..5448
                     /locus_tag="PC472_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2729460.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(5614..6150)
                     /locus_tag="PC472_004250"
     CDS             complement(5614..6150)
                     /locus_tag="PC472_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2729461.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(6263..6937)
                     /locus_tag="PC472_004251"
     CDS             complement(6263..6937)
                     /locus_tag="PC472_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2729462.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
BASE COUNT         2650 a          822 c         1170 g         2538 t
ORIGIN      
        1 aggaaaaact tgagcgtatt tttgaacaat tttacaggtt ggatacatct cgtaattcaa
       61 acagtggtag agctgggttg ggacttgcta tagctaaaga aatagtggag cttcataacg
      121 ggacaataac agctcatagc gagaacaata taattatctt taaggttatt attccgattg
      181 taggaaattt gtaagaattt aagtataaaa aaataggaaa ttctttagga taaataaata
      241 aagagcactc ttgttattga tataattact gtatcaaaac aagagtgctt ttattttgat
      301 acagtaatta tatcaataga aaaatgtgaa aatatattat gtggaacaga tgcaattgta
      361 agtgaaggtt gattggattt gaaatagaaa aagtatttta acatgtttct gtagattgta
      421 taaaaataaa taaatcaaca aaggagaatc aaagatgaat aataaaaaaa ttgcagttat
      481 atttggtgga aactcaacag aatatgatgt atcattacaa tcagcttttg cagtacttga
      541 gaatttgaat acggaaaaat atgatattat accaattgga attacaaggg agggtaattg
      601 gtatcattat attggagatt ataataatat ccagaataac acttggtttg aaagggatac
      661 tgatataatt ccagttattg tttcgcagaa ccgtgtcaaa aatggtatta ttgaattttt
      721 aattcctgat gctgtttctc agaatcatat tccaaatagt gctattgaat catgtgaaaa
      781 tgatattaaa tacactaaac ttgattttgt gtttcctgta ctgcatggaa aaaatggaga
      841 agatggaaca gtgcaggggt tatttgaatt ggcaggtata caggttatag gatgtggtac
      901 tctttcttca gcattatgta tggacaaaga caaatcacat aaacttgttc attcagaagg
      961 tattaaagta ccaaaatcaa tattactaaa atactcagat aaatttaatg tggctgaact
     1021 gtcaaaagac ttaacttatc cattatttat aaaacctgtg cgtgcaggtt catcatttgg
     1081 tattagtaaa atttataaaa aagaagagtt acaagtagca gtagacttgg cttttgaaca
     1141 tgataatgaa gtaataattg aggaaaatat tgatggtttt gaggtagggt gtgcagtttt
     1201 aggaaacgat gacttacttg ttggaagagt agatgaaata gaattgtcaa aaggattttt
     1261 tgattataca gaaaaatata cattgaaaag ttcaaaaatt catatgccag ctagaattga
     1321 tttggattgt gagaaaagaa tacaagagac agcaaaaatt atttatagaa ctcttggatg
     1381 ttctggattt gctagagtag atatgttttt aactccatct ggtgaaatta tttttaatga
     1441 agtcaataca attcctggct ttacttctca tagtcgttat ccaaatatga tgaaaggtat
     1501 tggattgtct tttgaagaaa tattggacaa gataattgag gtgtacataa aatgatgaat
     1561 aatattttat tagataaaaa taaagaagat aaaatgacaa atactatctt attgactaaa
     1621 gataaagttt atgatgggaa tctaattcta gtaaatgctt ttttaccagt taaaacatct
     1681 gaagatatag atttaattcc tgtagataca aggtttccaa gtattcttat gaaacgtgaa
     1741 gctacaaaca ttttacaaaa tattttaaaa agtatatgtg gaataaatga aatagtacct
     1801 gtaagtggat atcgtactgc agaggaacaa caagatatat attcaagttc tctgcgtgac
     1861 aatggaaaag actttactaa aaagtttgta gcacttccaa atcacagtga acatcagaca
     1921 gggcttgcta ttgatttagg gttaaagaaa gaagttatag attttatctg ccctgaattt
     1981 ccttataatg gtatttgtaa tgagtttcgt aaggcagcac tacattatgg ttttatagag
     2041 cgttatcagt gtaaaaaaga agaaataaca ggaatatctc aagaaccatg gcattttcgt
     2101 tatgtgggat atccgcattc tgagattatc aataaaatga atctcgctct tgaagaatat
     2161 atagaatata ttaagagatt ttcgtataca aattgtttaa aaatagaaga taaagaaaaa
     2221 attatagaaa tcttttatgt gcctattcat actaaagaag aagtgagaat ctctatacct
     2281 gaacaatgtg tatatcaaat atcgggtaac aataaggatg gttttattgt gacattttgg
     2341 agaaatagaa aggactaaga aatggaggaa caagaatgat taggaataaa tcttatgcag
     2401 ggcttgatta ttttaggatt atagctgcat tacttataat tgcaattcat acatctccat
     2461 tattatcata tagtaaaatg gctgactttg tgttaactag tataattgct cgtattgctg
     2521 tgccattctt ttttatgcta tcaggctttt ttctaatttc aacatataat tataattgta
     2581 ataaactgaa atcttttcta aaaaatgtat tatttattta cgggatagct attgtaattt
     2641 atattccctt aaatatttac actggatatt tttccatgaa taatttagtg cccaatatta
     2701 ttaaggacat tgtatttgat gggactttat atcatttatg gtatctacct gcatcaatta
     2761 ttggtgcaag tattgcatac tttcttgtaa aaagactagg tttaaagtac tcactagtaa
     2821 tcactattgt tatttatgta attggtatgt ttggtgatag ttattatggt tttttagaaa
     2881 aaataccact attaaagaat ttatatgggt atatttttga agtatcagat tatacacgaa
     2941 atggtatatt ttttgcacct gtatttttca tacttggtgg aataatatct aataaatcta
     3001 attatatgtc aactaaaaat agcttgattg gatttcttat atcattctta cttatgttat
     3061 gtgagggaat gtttttacat aaactagggg tacagcgtca tgatagcatg tatattatgt
     3121 tattgccatg tatgtatttc ttatttacag ccttaacatt tttgaaaggt tctcgagcta
     3181 ggttattgcg aacatcatca ttaattattt atattataca tcctatgatg attgttgtta
     3241 ttcgtatgtt ttctaaaata ttcggaatac aaactgtctt ggttgacaat agccttattc
     3301 attatatagt ggtaagtata gcatctttta tagcttctat aattattata ttgattctta
     3361 aacctctaaa aaaagacttg aaaggagaac ataaggtaaa taccaataga gcttggattg
     3421 aaattgattt aaataatttg gaacataatg taaagatgat acaaaaagct atgccagaag
     3481 attgtgaatt gatggcagtt gtaaaagcta atgcctatgg tcatagtgct tttgaagtag
     3541 cgacttgtgc aaaccaaata ggtgtaaaag catttgcagt agcaacaata gatgagggga
     3601 ttgatttacg tcgatatgga atactaggag aaatattgat tcttggatat actaacccaa
     3661 taagggcaaa ggaacttcat aaatacaatc ttacacagag tgttattgac cttaatcatg
     3721 ctatctcttt aaacaaacag aaatacaaca taaaatcaca cattaaaatt gatacaggta
     3781 tgcacagact gggttttgat gttaatgatg tgaggtcaat tttaaaggca tttaaattaa
     3841 aatacttaga tatatgtggc atatttacac acttgtgtgt ttcagatagt ctgaaagatg
     3901 aagatgtaga ctttacaaat aaacaaattg aatgttttta taagttaatt gattttctgt
     3961 taaaaaaagg tataaaaatt cctaaagtac atattcaaag tagttatggt ttttttaatt
     4021 atcccaatct taagtgtaat tatgtaagaa ttggaattgc attatacggg atattaagtt
     4081 ctccaaatga tacgactaaa ttgcaacttg atttacgacc tgttttatcc ttaaaatcac
     4141 aagtaattct tattagaaaa atacaaaaag atgatagttt tggatatgga agagtcttta
     4201 ttgcaagcag agatagcgta attgctatat tatctattgg ctatgccgat ggtataccta
     4261 gaaatttatc ctgtggaaaa agttacgtaa taataaatgg ttgtagagca gcaatagtgg
     4321 gtcgaatttg catggaccaa cttgctgttg atattacaga tatacctaat gtagaagttg
     4381 gtaatacggc aataataata ggaagagata atttatctga attatctgct tctgaagttg
     4441 caaataactc atgtagtata tcgaatgaat tattgagcag agtaggaaga aggttaaatg
     4501 ttataaagaa gtaactcaat taaatagtaa tatttgttgt actgtgtaat ttattttact
     4561 ctgactagac atttttagta atattatgtc taatatatat tttttataac ttacatgaca
     4621 atttaagtta taaaaaggtg atgtttcaga tttttatttt gaaacatcac cttttttgtt
     4681 ataatatata ataaaataat tataccttaa ctgtaaactt atatagatta tttacttttt
     4741 aatattagat tcattttttg agattggtat ataataaaca tatagagtat ataataatag
     4801 ttaaagttaa aaaaggggaa aagcaatatg cagtatatac aagaactact tgatttgact
     4861 caaaattatt gggtgttagc cacaataatc ggcttattaa gtgcatttat agaaagcttt
     4921 ataccagcct taccacttgt tgctatagtt acagcaaatg ctgcaataca aggattattt
     4981 attggatgtt tgttatcatg gattggctca ggacttggga caacttcatt attcttatta
     5041 ataagcagat ttaatgatag taagcttttt aataagttga gaaatagtaa aactgaaaaa
     5101 gcaataagtt ggatggataa acagggattt aagttattat ttatagcgta tgcctgtcca
     5161 tttatgcccg gttgtttagt tactatcgca tcagcttttg gtaaaaaaga tttaaaagat
     5221 tttgtgccag caatgttggc aggaaaattt gtaatgttta tagttataag ttacgtggca
     5281 agtgatatag aaggttttat aacaagtcct ttaaagatag catcatttat attattggtg
     5341 tttttgtcat ggaagattgg aaatagagta aataaaaatc ttgaaaatca taattatgat
     5401 ttccatcata aaaaacatca caatgataaa gatgataaaa tgatttagct tgtacgaaat
     5461 aaaacgtatc ttaaaatgat ttagtactaa attattttaa gatacgtttt attttttgta
     5521 ttaaggtaca aaatttttag ttcttttgta aactaaaata tataaaactt atttatactt
     5581 agttcaaaat attgttattt attttttcta caactataat aaaatataaa attgatatcc
     5641 aatcaaaaaa ataactatga aatttaatag cttagatatg aatttttctg tttttgagtt
     5701 agtttttata ggtagtaaag aaatgtttat ggtaatggga taaaacaatt caataccttc
     5761 tttagtaata aggtcaagac acaagtgaga tagatatcct attataaacc cactggacaa
     5821 acatgagaaa actatattat tgttagtgaa gattgtcaga gatttaaaaa aactataaat
     5881 aagtaataag gcaattagac tgtgagtaaa ccctctatgt ctaaacctac ttccaaataa
     5941 tttgtatatt aacataaatt ttttacttat gtaagaacct ctcatatcta tatctggaaa
     6001 taaagaacct aaatatgcaa aataaatata aattataatt aatactattt tatatgcaat
     6061 atcgtattta actaggtata gattgtttat aaaggggagt atcaaaagtg cgaatatata
     6121 acctcccctt tcatgtgttt cttttatcat ataaaaatcc tcctgaatcg tcactataaa
     6181 cattatattg gtaaatttag actatctttt attatattat atagcaaaca taagtttttt
     6241 tcaaaaggat tttaatattt aattaatctt gagattttaa atttttgtaa ttttctaaag
     6301 caccattcca atttactaca ttaaaccagt tgtcaatgta ttcatttctt ctattttgat
     6361 attttaaata gtaagcatgc tcccaaacat caagtcctat tataggagtt aggtttttac
     6421 ttacagggct atcctgattt ggagtagtca taatagataa tttgccatct ttagtagcta
     6481 caagccaagc ccaaccagaa ccaaagacat ctaaagcaga tttttggaac tcttgcttaa
     6541 atttttcaaa agaaccaaag tctctatcaa tagcttcttt taaagattca gaaggtatgg
     6601 ttttttctgg cgtcattata tcaaaaaaga atttatgatt ataagctcca cctgcattat
     6661 ttcttacagt tgtagcaata tctttaggta aagaatctaa attttgcaat aattcacata
     6721 aagaataatt ataaagctca ggatattttt caagagcagc atttaattta tcaacataag
     6781 cttgataatg cttatcatga tgcagtttca ttgtttcttt atctatataa ggttcaagtg
     6841 catcatatgc ataaggtaat ggtttaacct taaatttgtt attttcaggt gtaaaagcaa
     6901 atgaagttat gcactgtgag attataaata aagacataat aactggtatt aatatttttt
     6961 tcttcataaa aatcacctcc taatatagta tttctagaaa taaaaaaaat atgaaatatt
     7021 aacagataat ttataagaat ttaatagcaa tttcatttta aattgaataa gttttaaagc
     7081 caaagacaaa actatgaaat atagaataag actaaaggac agaaaggaat tttgattata
     7141 tggacggcaa tatttcaatt tattataaag gtgatatatc
//
LOCUS       DAMJYC010000290         3327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.209, whole genome shotgun sequence.
ACCESSION   DAMJYC010000290 DAMJYC010000000
VERSION     DAMJYC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.209"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..1060
                     /locus_tag="PC472_004252"
     CDS             <1..1060
                     /locus_tag="PC472_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2729463.1"
                     /translation="IAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKAALNLYQQI
                     FRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEVALSPELLA
                     IEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTLLRDESQIS
                     EELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQLTYEFKP
                     TLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQEDDYI
                     KLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTITAH
                     SENNIIIFKVIIPIVGNL"
     gene            1321..2421
                     /gene="vanG"
                     /locus_tag="PC472_004253"
     CDS             1321..2421
                     /gene="vanG"
                     /locus_tag="PC472_004253"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2729464.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            2418..3224
                     /locus_tag="PC472_004254"
     CDS             2418..3224
                     /locus_tag="PC472_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2729465.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
BASE COUNT         1246 a          377 c          599 g         1105 t
ORIGIN      
        1 tatagccatt attttcatat ggattttata ttcttttata ttgtatggta agttttctat
       61 ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg cattgaattt
      121 atatcagcag attttccgta atagcatgga gctcttcatg atactagcta tggtgattgt
      181 gttttttata atattccggg tttatttaaa ttggtttaca aaatatttta gtgaaataaa
      241 tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat ctccagagtt
      301 attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac gtaaatttga
      361 gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac atgatttaaa
      421 aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa gtcagatttc
      481 tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc gtcttgaaga
      541 tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat cacttgaata
      601 cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta aacctacttt
      661 gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga taaggtgtga
      721 tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt attatagttt
      781 tgaagatact actattgaaa taacagctgt acaggaggat gattatataa agcttaaatt
      841 tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg aacaatttta
      901 caggttggat acatctcgta attcaaacag tggtagagct gggttgggac ttgctatagc
      961 taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga acaatataat
     1021 tatctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt ataaaaaaat
     1081 aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata attactgtat
     1141 caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa tgtgaaaata
     1201 tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa tagaaaaagt
     1261 attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg agaatcaaag
     1321 atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata tgatgtatca
     1381 ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga tattatacca
     1441 attggaatta caagggaggg taattggtat cattatattg gagattataa taatatccag
     1501 aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc gcagaaccgt
     1561 gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa tcatattcca
     1621 aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga ttttgtgttt
     1681 cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt tgaattggca
     1741 ggtatacagg ttataggatg tggtactctt tcttcagcat tatgtatgga caaagacaaa
     1801 tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt actaaaatac
     1861 tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt atttataaaa
     1921 cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga agagttacaa
     1981 gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga aaatattgat
     2041 ggttttgagg tagggtgtgc agttttagga aacgatgact tacttgttgg aagagtagat
     2101 gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt gaaaagttca
     2161 aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca agagacagca
     2221 aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat gtttttaact
     2281 ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac ttctcatagt
     2341 cgttatccaa atatgatgaa aggtattgga ttgtcttttg aagaaatatt ggacaagata
     2401 attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa gaagataaaa
     2461 tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta attctagtaa
     2521 atgctttttt accagttaaa acatctgaag atatagattt aattcctgta gatacaaggt
     2581 ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt ttaaaaagta
     2641 tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag gaacaacaag
     2701 atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag tttgtagcac
     2761 ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta aagaaagaag
     2821 ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag tttcgtaagg
     2881 cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa ataacaggaa
     2941 tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag attatcaata
     3001 aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg tatacaaatt
     3061 gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct attcatacta
     3121 aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg ggtaacaata
     3181 aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg gaggaacaag
     3241 aatgattagg aataaatctt atgcagggct tgattatttt aggattatag ctgcattact
     3301 tataattgca attcatacat ctccatt
//
LOCUS       DAMJYC010000291         3327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.210, whole genome shotgun sequence.
ACCESSION   DAMJYC010000291 DAMJYC010000000
VERSION     DAMJYC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.210"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            1..1060
                     /locus_tag="PC472_004255"
                     /pseudo
     CDS             1..1060
                     /locus_tag="PC472_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            1321..2421
                     /gene="vanG"
                     /locus_tag="PC472_004256"
     CDS             1321..2421
                     /gene="vanG"
                     /locus_tag="PC472_004256"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2729466.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            2418..3224
                     /locus_tag="PC472_004257"
     CDS             2418..3224
                     /locus_tag="PC472_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2729467.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
BASE COUNT         1246 a          377 c          598 g         1106 t
ORIGIN      
        1 tatagccatt attttcatat ggattttata ttcttttata ttgtatggta agttttctat
       61 ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg cattgaattt
      121 atatcagcag attttccgta atagcatgga gctcttcatg atactagcta tggtgattgt
      181 gttttttata atattccggg tttatttaaa ttggtttaca aaatatttta gtgaaataaa
      241 tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat ctccagagtt
      301 attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac gtaaatttga
      361 gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac atgatttaaa
      421 aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa gtcagatttc
      481 tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc gtcttgaaga
      541 tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat cacttgaata
      601 cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta aacctacttt
      661 gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga taaggtgtga
      721 tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt attatagttt
      781 tgaagatact actattgaaa taacagctgt acaggaggat gattatataa agcttaaatt
      841 tataaatcat agcaatacga ttccatagga aaaacttgag cgtatttttg aacaatttta
      901 caggttggat acatctcgta attcaaacag tggtagagct gggttgggac ttgctatagc
      961 taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga acaatataat
     1021 tatctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt ataaaaaaat
     1081 aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata attactgtat
     1141 caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa tgtgaaaata
     1201 tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa tagaaaaagt
     1261 attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg agaatcaaag
     1321 atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata tgatgtatca
     1381 ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga tattatacca
     1441 attggaatta caagggaggg taattggtat cattatattg gagattataa taatatccag
     1501 aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc gcagaaccgt
     1561 gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa tcatattcca
     1621 aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga ttttgtgttt
     1681 cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt tgaattggca
     1741 ggtatacagg ttataggatg tggtactctt tcttcagcat tatgtatgga caaagacaaa
     1801 tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt actaaaatac
     1861 tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt atttataaaa
     1921 cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga agagttacaa
     1981 gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga aaatattgat
     2041 ggttttgagg tagggtgtgc agttttagga aacgatgact tacttgttgg aagagtagat
     2101 gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt gaaaagttca
     2161 aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca agagacagca
     2221 aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat gtttttaact
     2281 ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac ttctcatagt
     2341 cgttatccaa atatgatgaa aggtattgga ttgtcttttg aagaaatatt ggacaagata
     2401 attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa gaagataaaa
     2461 tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta attctagtaa
     2521 atgctttttt accagttaaa acatctgaag atatagattt aattcctgta gatacaaggt
     2581 ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt ttaaaaagta
     2641 tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag gaacaacaag
     2701 atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag tttgtagcac
     2761 ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta aagaaagaag
     2821 ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag tttcgtaagg
     2881 cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa ataacaggaa
     2941 tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag attatcaata
     3001 aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg tatacaaatt
     3061 gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct attcatacta
     3121 aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg ggtaacaata
     3181 aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg gaggaacaag
     3241 aatgattagg aataaatctt atgcagggct tgattatttt aggattatag ctgcattact
     3301 tataattgca attcatacat ctccatt
//
LOCUS       DAMJYC010000292         2188 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,16.7537597 isolate JH469
            SAMN32366281-rid16592153.guided.233, whole genome shotgun sequence.
ACCESSION   DAMJYC010000292 DAMJYC010000000
VERSION     DAMJYC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366281
            Sequence Read Archive: SRR22862960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560816.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560816.1
            Genome Coverage       :: 221x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:34:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,259
            CDSs (total)                      :: 4,169
            Genes (coding)                    :: 4,090
            CDSs (with protein)               :: 4,090
            Genes (RNA)                       :: 90
            rRNAs                             :: 8, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 32 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 22 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366281-rid16592153.guided.233"
                     /strain="L,16.7537597"
                     /isolate="JH469"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Spain"
                     /collection_date="2011"
     gene            <1..982
                     /locus_tag="PC472_004258"
     CDS             <1..982
                     /locus_tag="PC472_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2729468.1"
                     /translation="DWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGS
                     RNFIPPIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGL
                     EVVVVEAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDG
                     DSVDCDMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNIS
                     LAIWPSSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKD
                     EGQRIYKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            1416..2117
                     /locus_tag="PC472_004259"
     CDS             1416..2117
                     /locus_tag="PC472_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2729469.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
BASE COUNT          846 a          196 c          421 g          725 t
ORIGIN      
        1 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
       61 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
      121 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
      181 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
      241 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
      301 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
      361 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
      421 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
      481 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
      541 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
      601 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
      661 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
      721 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
      781 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
      841 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
      901 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
      961 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     1021 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     1081 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     1141 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     1201 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     1261 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     1321 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     1381 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     1441 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     1501 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     1561 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     1621 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     1681 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     1741 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     1801 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     1861 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     1921 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     1981 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     2041 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     2101 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     2161 atttttatga gaacagcttt aatggcat
//
